title: Development of synchrotron radiation x-ray intravital microscopy for in vivo imaging of rat heart vascular function.
abstract: This study elucidates the vascular internal diameter response of coronary arterial circulation in closed-chest rats to evaluate endothelium-dependent and endothelium-independent vasodilatory ability and to investigate disease mechanisms. For this study, we developed an X-ray intravital microscopy system using a microangiography technique and a synchrotron radiation source at SPring-8. An X-ray direct-conversion type detector with 7-μm spatial resolution was used for real-time imaging. Microangiographic images were stored in a digital frame memory system at a maximum rate of 30 frame/s with a 1024 × 1024-pixel, 10-bit format. In imaging experiments, the small coronary arteries were visualized after iodine contrast agent injection into the coronary artery.
authors.affiliation: Japan Synchrotron Radiation Research Institute, Sayo-gun, Hyogo 679-5198, Japan. umetani@spring8.or.jp
authors.forename: Keiji
authors.initials: K
authors.lastname: Umetani
authors.forename: James T
authors.initials: JT
authors.lastname: Pearson
authors.forename: Daryl O
authors.initials: DO
authors.lastname: Schwenke
authors.forename: Mikiyasu
authors.initials: M
authors.lastname: Shirai
mesh_terms: D000818:Animals; D017023:Coronary Angiography; D003331:Coronary Vessels / Q000502:physiology*; D006321:Heart / Q000502:physiology*; D006334:Heart Function Tests / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008833:Microcirculation; D008853:Microscopy / Q000379:methods*; D051381:Rats; D017356:Synchrotrons; D014965:X-Rays