title: [Study on HPLC chromatographic fingerprint of anti-tumor active site SSCE of Caulis spatholobi].
abstract: OBJECTIVE
  To establish the chromatographic fingerprints for the anti-tumor flavonoids of Caulis spatholobi (SSCE). It could used to reflect the chemical information in this part comprehensively, and identify the chemical consitituents preliminarily.
  
  
  METHODS
  The HPLC-DAD analysis method was performed on the column Kromasil 100-5PHENYL (4.6 mm x 250 mm, 5 microm). The mobile phase was water (0.5% acetic acid)- methanol in gradient elution and the detection wavelength was 254 nm.
  
  
  RESULTS
  The chromatographic fingerprint of SSCE was established, which showed 16 characteristic peaks from 10 batches of medicinal materials. Among them, the peaks 1, 3, 4, 5, 8, 9, 10, 12, 13, and 16 were identified 3,4-dihodroxybenzoic acid, 4-Hydroxybenzoic Acid, epicatechin, puerarin, daidzein, liquiritigenin, calycosin, genistein, formononetin, and prunetin, respectively.
  
  
  CONCLUSIONS
  The method is convenient, reproducibility and stability. It can used for quality control of the anti-tumor flavonoids of C. spatholobi (SSCE).
authors.affiliation: Beijing Institute of Traditional Chinese Medicine, Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University, Beijing 100010, China. whpgsl030701@sina.com
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Yina
authors.initials: Y
authors.lastname: Liu
authors.forename: Zuping
authors.initials: Z
authors.lastname: Zeng
authors.forename: Wei
authors.initials: W
authors.lastname: He
mesh_terms: D000970:Antineoplastic Agents / Q000032:analysis* / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry*; D007887:Fabaceae / Q000737:chemistry* / Q000378:metabolism; D005419:Flavonoids / Q000032:analysis / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry; D011786:Quality Control; D015203:Reproducibility of Results
chemical_list: D000970:Antineoplastic Agents; D004365:Drugs, Chinese Herbal; D005419:Flavonoids