title: [Establishment of primary Her-2 over-  expression human breast cancer cell  model].
abstract: OBJECTIVE
  Through isolation and purification pri-mary HER2 overexpression human breast cancer cells from malignant pleural effusion and identification the HER2 expression level of the cells to establish the primary HER2 overexpression human breast cancer cell model. METH-ODS: Malignant pleural effusion of HER2 overexpression breast cancer patient was collected. The primary cells were extracted from malignant pleural effusion by Lymphocyte separation medium and the method of density gradient centrifugation. When the primary cells were cultured and spreaded to the 5th generation, the HER2 expression level of the primary cells were detected by the methods of Q-PCR,Western blot and flow cytometry (FCM). Ability of tumor-bearing was detected by tumor-bearing nude mice assay.
  
  
  RESULTS
  The primary HER2 overexpression human breast cancer cells were extracted and identified by the methods of Q-PCR, Western blot and tumor-bearing nude mice assay,even though the FCM showed Negative results.
  
  
  CONCLUSIONS
  The primary HER2 overexpression human breast cancer cell model was established; Identification of primary cells need to be confirmed by different methods.
authors.affiliation: Department of Oncology, First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Li-juan
authors.initials: LJ
authors.lastname: Wang
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhao
authors.forename: Hua-wei
authors.initials: HW
authors.lastname: Xiao
authors.forename: E
authors.initials: E
authors.lastname: Bai
authors.forename: Chao
authors.initials: C
authors.lastname: Dong
authors.forename: Tao
authors.initials: T
authors.lastname: Yang
authors.forename: An-gang
authors.initials: AG
authors.lastname: Yang
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhu
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D016066:Pleural Effusion, Malignant / Q000235:genetics / Q000473:pathology; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D018719:Erb-b2 Receptor Tyrosine Kinases