title: The 40-80 nt region in the 5'-NCR of genome is a critical target for inactivating poliovirus by chlorine dioxide.
abstract: Chemical disinfection is the most common method used to inactivate viruses from drinking water throughout the world. In this study, cell culture, ELISA, RT-PCR, and spot hybridization were employed to investigate the mechanism underlying chlorine dioxide (ClO(2) )-induced inactivation of Poliovirus type 1 (PV1), which was also confirmed by recombinant viral genome RNA infection models. The results suggested that ClO(2) inactivated PV1 primarily by disrupting the 5'-non-coding region (5'-NCR) of the PV1 genome. Further study revealed that ClO(2) degraded specifically the 40-80 nucleotides (nt) region in the 5'-NCR. Recombinant viral genome RNA infection models confirmed that PV1 RNA lacking this 40-80 nt region was not infectious. This study not only elucidated the mechanism of PV1 inactivation by ClO(2), but also defined the critical genetic target for the disinfectant to inactivate Poliovirus. This study also provides a strategy by which rapid, accurate, and molecular methods based on sensitive genetic targets may be established for evaluating the effects of disinfectants on viruses.
authors.affiliation: Department of Environment and Health, Institute of Health and Environmental Medicine, Key Laboratory of Risk Assessment and Control for Environment and Food Safety, Tianjin, China.
authors.forename: Min
authors.initials: M
authors.lastname: Jin
authors.forename: Zu-Guo
authors.initials: ZG
authors.lastname: Zhao
authors.forename: Xin-Wei
authors.initials: XW
authors.lastname: Wang
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Shen
authors.forename: Lu
authors.initials: L
authors.lastname: Xu
authors.forename: Yun-Mei
authors.initials: YM
authors.lastname: Yu
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Qiu
authors.forename: Zhao-Li
authors.initials: ZL
authors.lastname: Chen
authors.forename: Jing-Feng
authors.initials: JF
authors.lastname: Wang
authors.forename: Ai-Hua
authors.initials: AH
authors.lastname: Huang
authors.forename: Jun-Wen
authors.initials: JW
authors.lastname: Li
mesh_terms: D020121:5' Untranslated Regions; D017606:Chlorine Compounds / Q000494:pharmacology*; D004202:Disinfectants / Q000494:pharmacology*; D004203:Disinfection; D016679:Genome, Viral; D006367:HeLa Cells; D006801:Humans; D010087:Oxides / Q000494:pharmacology*; D017955:Poliovirus / Q000187:drug effects* / Q000235:genetics* / Q000276:immunology; D038301:Virus Inactivation / Q000187:drug effects*
chemical_list: D020121:5' Untranslated Regions; D017606:Chlorine Compounds; D004202:Disinfectants; D010087:Oxides; C025109:chlorine dioxide