title: Mouse splenocyte blast transformation in the presence of plasma γ-globulin fraction proteins and their complexes with copper and zinc.
abstract: Plasma γ-globulin fraction proteins, copper and zinc cations, and metal complexes formed by these cations and human serum γ-globulin induce blast transformation of splenocytes from BALB/c mice at a level comparable to that induced by concanavalin A. Zinc bound to γ-globulin reduces by 25% and copper in complex with this protein stimulates by 1.6 times its capacity to induce blast transformation. Combinations with concanavalin A reproduce the effects of γ-globulin-metal complex under conditions of mitogen induction. Incorporation of(3)H-thymidine in splenocytes incubated with combinations of γ-globulin-copper metalcomplex, copper cations, and control protein with concanavalin A was by 1.4, 1.3 (p<0.1), and 1.25 times higher (p<0.05), respectively, than after incubation with concanavalin A alone.
authors.affiliation: Laboratory of Cell-to-Cell Interactions, N. F. Gamaleya Institute of Epidemiology and Microbiology, Ministry of Health and Social Development of Russian Federation, Moscow, Russia. cheknev@gamaleya.org
authors.forename: S B
authors.initials: SB
authors.lastname: Cheknev
authors.forename: E A
authors.initials: EA
authors.lastname: Grigorjeva
authors.forename: T N
authors.initials: TN
authors.lastname: Nikolaeva
authors.forename: A V
authors.initials: AV
authors.lastname: Pronin
mesh_terms: D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology; D056831:Coordination Complexes / Q000097:blood; D003300:Copper / Q000097:blood*; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D008934:Mitogens; D013154:Spleen / Q000166:cytology*; D013936:Thymidine / Q000378:metabolism; D015032:Zinc / Q000097:blood*; D005719:gamma-Globulins / Q000378:metabolism*
chemical_list: D056831:Coordination Complexes; D008934:Mitogens; D005719:gamma-Globulins; D003208:Concanavalin A; D003300:Copper; D015032:Zinc; D013936:Thymidine