title: Chondrogenesis in scaffolds with surface modification of elastin and poly-L-lysine.
abstract: A tissue engineering cartilage is of great importance in the current diarthrodial surgery. This study presents the formation of neocartilage by cultivating chondrocytes in elastin- and poly-L-lysine-modified scaffolds. The hybrid bulk biomaterials used contained polyethylene oxide, chitin, and chitosan and were fabricated by crosslinking, pre-freezing, and lyophilization. Bovine knee chondrocytes were seeded in the scaffolds and cultured in a spinner-flask bioreactor over 4 weeks. Surface elastin showed a better efficiency in the adhesion and proliferation of bovine knee chondrocytes in the scaffolds than surface poly-L-lysine. In addition, elastin-modified constructs yielded higher quantities of secreted glycosaminoglycans and produced collagen than poly-L-lysine-modified constructs. The surface morphology demonstrated a thriving chondrogenesis in the two kinds of constructs. The staining images revealed that elastin induced larger amounts of regenerated bovine knee chondrocytes, glycosaminoglycans, and type II collagen in the constructs than poly-L-lysine. Elastin- and poly-L-lysine-grafted polyethylene oxide/chitin/chitosan scaffolds are effective in producing cartilaginous components.
authors.affiliation: Department of Chemical Engineering, National Chung Cheng University, Chia-Yi, Taiwan, ROC. chmyck@ccu.edu.tw
authors.forename: Yung-Chih
authors.initials: YC
authors.lastname: Kuo
authors.forename: Chiu-Yen
authors.initials: CY
authors.lastname: Chung
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D002356:Cartilage / Q000166:cytology / Q000187:drug effects; D002417:Cattle; D018929:Cell Culture Techniques; D002686:Chitin / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry; D019902:Chondrocytes / Q000187:drug effects; D020219:Chondrogenesis / Q000187:drug effects; D024043:Collagen Type II / Q000096:biosynthesis; D003432:Cross-Linking Reagents; D004549:Elastin / Q000737:chemistry* / Q000494:pharmacology; D005612:Freeze Drying; D006025:Glycosaminoglycans / Q000096:biosynthesis; D006801:Humans; D011092:Polyethylene Glycols / Q000737:chemistry; D011107:Polylysine / Q000737:chemistry* / Q000494:pharmacology; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D024043:Collagen Type II; D003432:Cross-Linking Reagents; D006025:Glycosaminoglycans; D002686:Chitin; D011107:Polylysine; D011092:Polyethylene Glycols; D004549:Elastin; D048271:Chitosan