title: Aronia melanocarpa extract suppresses the biotoxicity of homocysteine and its metabolite on the hemostatic activity of fibrinogen and plasma.
abstract: OBJECTIVE
  Aronia melanocarpa fruits (Rosaceae) are one of the richest plant sources of phenolic substances, and it has been shown to have various biological activities. Berries of A. melanocarpa (chokeberry) have been supposed to be beneficial for the prevention of cardiovascular events. In this study the influence of aronia extract on the clot formation (using human plasma and purified fibrinogen) and the fibrin lysis during the model of hyperhomocysteinemia was investigated.
  
  
  METHODS
  Hyperhomocysteinemia was induced using a reduced form of Hcys (at final dose of 0.1mM) and the most reactive form of Hcys - its cyclic thioester, homocysteine thiolactone (HTL, 1 μM). The aim of our study in vitro was also to investigate the modifications of human plasma total proteins and the oxidative stress (by measuring the total antioxidant level - TAS) in plasma after incubation with Hcys, HTL and/or aronia extract. The biological properties of aronia extract were compared with the action of a well characterized antioxidative commercial polyphenol - resveratrol (3,4',5- trihydroxystilbene).
  
  
  RESULTS
  The HTL, like its precursor, Hcys stimulated polymerization of fibrinogen. The results also demonstrated that Hcys (0.1mM) and HLT at lower doses than Hcys (1 μM) reduced the fibrin lysis in human plasma. Moreover, Hcys and HTL change the level of thiol and amino groups in plasma total proteins and induce the oxidative stress in plasma. Our results indicate that aronia extract reduced the biotoxicity action of Hcys and HTL on hemostatic properties of fibrinogen or plasma, suggesting its possible protective properties in hyperhomocysteinemia - induced cardiovascular diseases. Moreover, our results showed that the extract from berries of A. melanocarpa due to antioxidant action, significantly reduced the oxidative stress (measured by TAS) in plasma during the model of hyperhomocysteinemia.
  
  
  CONCLUSIONS
  In the comparative studies, the extract from berries of A. melanocarpa and reseveratrol had similar protective properties. It gives hopes for development of diet supplements, which may be preventing thrombosis in pathological states where plasma procoagulant activity and oxidative stress are observed e.g. in hyperhomocysteinemia.
authors.affiliation: Department of General Biochemistry, University of Łódź, Łódź, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Malinowska
authors.forename: Karolina
authors.initials: K
authors.lastname: Babicz
authors.forename: Beata
authors.initials: B
authors.lastname: Olas
authors.forename: Anna
authors.initials: A
authors.lastname: Stochmal
authors.forename: Wieslaw
authors.initials: W
authors.lastname: Oleszek
mesh_terms: D000328:Adult; D000925:Anticoagulants / Q000494:pharmacology* / Q000627:therapeutic use; D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use; D001777:Blood Coagulation / Q000187:drug effects*; D002318:Cardiovascular Diseases / Q000209:etiology / Q000517:prevention & control; D003029:Coagulants / Q000037:antagonists & inhibitors / Q000494:pharmacology; D019587:Dietary Supplements; D005340:Fibrinogen / Q000737:chemistry / Q000378:metabolism*; D005638:Fruit / Q000737:chemistry / Q000254:growth & development; D006710:Homocysteine / Q000031:analogs & derivatives / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D006801:Humans; D020138:Hyperhomocysteinemia / Q000097:blood / Q000178:diet therapy / Q000503:physiopathology; D008954:Models, Biological; D018384:Oxidative Stress / Q000187:drug effects; D046936:Photinia / Q000737:chemistry* / Q000254:growth & development; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D011044:Poland; D058105:Polymerization / Q000187:drug effects; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology
chemical_list: D000925:Anticoagulants; D000975:Antioxidants; D003029:Coagulants; D010936:Plant Extracts; D013267:Stilbenes; D006710:Homocysteine; D005340:Fibrinogen; C007957:homocysteine thiolactone; D000077185:Resveratrol