title: In vitro and in vivo evaluation of ribavirin and pleconaril antiviral activity against enterovirus 71 infection.
abstract: Enterovirus 71(EV71) causes recurring outbreaks of hand, foot and mouth disease and encephalitis leading to complications or death in young children. More effective antiviral drugs are needed to prevent or reduce EV71-related disease and complications. However, there are no standard models currently in use to evaluate activity against EV71 infection both in vitro and in vivo. In this study, the activity of ribavirin and pleconaril against EV71 infection was evaluated in two models. An in vitro EV71 infection model was developed in RD cells, and an in vivo EV71 infection model was applied. Ribavirin and pleconaril effectively increased the viability of infected cells. Pleconaril reduced the morbidity and mortality of one-day-old infected mice, but ribavirin did not protect the infected mice. In all, the results demonstrated that infected cells and infected mice can be used to evaluate antiviral activity of ribavirin and pleconaril against EV71 infection in vitro and in vivo.
authors.affiliation: Department of Microbiology and Immunity, Nanjing Medical University, 140# Hanzhong Road, Nanjing, 210029, Jiangsu, China.
authors.forename: Guofeng
authors.initials: G
authors.lastname: Zhang
authors.forename: Feng
authors.initials: F
authors.lastname: Zhou
authors.forename: Bin
authors.initials: B
authors.lastname: Gu
authors.forename: Chuanling
authors.initials: C
authors.lastname: Ding
authors.forename: Dongju
authors.initials: D
authors.lastname: Feng
authors.forename: Fangyi
authors.initials: F
authors.lastname: Xie
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Wang
authors.forename: Chun
authors.initials: C
authors.lastname: Zhang
authors.forename: Qingxian
authors.initials: Q
authors.lastname: Cao
authors.forename: Yinlai
authors.initials: Y
authors.lastname: Deng
authors.forename: Weixing
authors.initials: W
authors.lastname: Hu
authors.forename: Kun
authors.initials: K
authors.lastname: Yao
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000998:Antiviral Agents / Q000494:pharmacology / Q000627:therapeutic use*; D045744:Cell Line, Tumor; D002470:Cell Survival; D002675:Child, Preschool; D004195:Disease Models, Animal; D029821:Enterovirus A, Human / Q000187:drug effects*; D004769:Enterovirus Infections / Q000188:drug therapy*; D006801:Humans; D051379:Mice; D008813:Mice, Inbred ICR; D008826:Microbial Sensitivity Tests; D010069:Oxadiazoles / Q000494:pharmacology / Q000627:therapeutic use*; D010080:Oxazoles; D012254:Ribavirin / Q000494:pharmacology / Q000627:therapeutic use*; D016019:Survival Analysis
chemical_list: D000998:Antiviral Agents; D010069:Oxadiazoles; D010080:Oxazoles; D012254:Ribavirin; C115201:pleconaril