title: An efficient protocol for the solid-phase synthesis of glycopeptides under microwave irradiation.
abstract: A standardized and smooth protocol for solid-phase glycopeptides synthesis under microwave irradiation was developed. Double activation system was proved to allow for highly efficient coupling of Tn-Ser/Thr and bulky core 2-Ser/Thr derivatives. Versatility and robustness of the present strategy was demonstrated by constructing a Mucine-1 (MUC1) fragment and glycosylated fragments of tau protein. The success of this approach relies on the combination of microwave energy, a resin consisting totally of polyethylene glycol, a low excess of sugar amino acid and the "double activation" method.
authors.affiliation: Graduate School of Life Science, Hokkaido University, N22, W11 Kita-ku, Sapporo, Japan.
authors.forename: Fayna
authors.initials: F
authors.lastname: Garcia-Martin
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hinou
authors.forename: Takahiko
authors.initials: T
authors.lastname: Matsushita
authors.forename: Shun
authors.initials: S
authors.lastname: Hayakawa
authors.forename: Shin-Ichiro
authors.initials: S
authors.lastname: Nishimura
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D006020:Glycopeptides / Q000138:chemical synthesis*; D006031:Glycosylation; D008872:Microwaves; D008969:Molecular Sequence Data; D015394:Molecular Structure; D060327:Solid-Phase Synthesis Techniques
chemical_list: D000596:Amino Acids; D006020:Glycopeptides