title: Autolytic degradation of skipjack tuna during heating as affected by initial quality and processing conditions.
abstract: Several factors were studied as affecting protein degradation and texture of skipjack tuna muscle following ambient pressure thermal processing (precooking). These included degree of mushy tuna syndrome (MTS) evidenced in the raw meat, raw meat pH, abusive thawing/holding, and precooking temperature/time. Slurries and intact pieces from frozen skipjack tuna, either tempered for 2 h or thawed and held at 25 °C for 22 h (abusive treatment) were heated at temperatures ranging from 40 to 80 °C for up to 2 h, and also at 90 °C for 1 h, with or without prior adjustment of pH to 5 or 7 to favor cathepsin or calpain activity, respectively. Proteolysis of precooked samples was monitored by Lowry assay and SDS-PAGE; cooked texture of intact meat was measured using a Kramer shear press and by sensory profile analysis. Proteolysis maximally occurred in slurries of skipjack tuna muscle that had been abusively stored (22 h at 25 °C) and adjusted to pH 5 prior to heating at 55 °C. Intact pieces of tuna abusively thawed/held for 22 h with subsequent heating at 55 °C also evidenced the most proteolysis and were the least firm in texture. Raw fish that evidenced higher severity of MTS when raw displayed higher levels of proteolysis prior to cooking, which were further increased after cooking at 55 °C.
  
  
  CONCLUSIONS
  The kinetic data presented here can be used to optimize processing conditions for skipjack tuna canning to minimize textural degradation and optimize quality.
authors.affiliation: Dow AgroSciences LLC, 9330 Zionsville Rd, Indianapolis, IN 46268, USA.
authors.forename: Nicola J
authors.initials: NJ
authors.lastname: Stagg
authors.forename: Penny M
authors.initials: PM
authors.lastname: Amato
authors.forename: Francis
authors.initials: F
authors.lastname: Giesbrecht
authors.forename: Tyre C
authors.initials: TC
authors.lastname: Lanier
mesh_terms: D000818:Animals; D002154:Calpain / Q000378:metabolism; D002403:Cathepsins / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005511:Food Handling / Q000379:methods*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D018482:Muscle, Skeletal / Q000378:metabolism; D059748:Proteolysis; D017747:Seafood; D014413:Tuna
chemical_list: D002403:Cathepsins; D002154:Calpain