title: Metabolic oligosaccharide engineering of Plasmodium falciparum intraerythrocytic stages allows direct glycolipid analysis by mass spectrometry.
abstract: A recent addition to the arsenal of tools for glycome analysis is the use of metabolic labels that allow covalent tagging of glycans with imaging probes. In this work we show that N-azidoglucosamine was successfully incorporated into glycolipidic structures of Plasmodium falciparum intraerythrocytic stages. The ability to tag glycoconjugates selectively with a fluorescent reporter group permits TLC detection of the glycolipids providing a new method to quantify dynamic changes in the glycosylation pattern and facilitating direct mass spectrometry analyses. Presence of glycosylphosphatidylinositol and glycosphingolipid structures was determined in the different extracts. Furthermore, the fluorescent tag was used as internal matrix for the MALDI experiment making even easier the analysis.
authors.affiliation: CIHIDECAR, Departamento de Química Orgánica, Pabellón II, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
authors.forename: Tamara
authors.initials: T
authors.lastname: Piñero
authors.forename: Valnice J
authors.initials: VJ
authors.lastname: Peres
authors.forename: Alejandro
authors.initials: A
authors.lastname: Katzin
authors.forename: Alicia S
authors.initials: AS
authors.lastname: Couto
mesh_terms: D002855:Chromatography, Thin Layer; D004912:Erythrocytes / Q000737:chemistry / Q000469:parasitology*; D005456:Fluorescent Dyes / Q000737:chemistry; D006017:Glycolipids / Q000032:analysis* / Q000737:chemistry; D006028:Glycosphingolipids / Q000737:chemistry; D006031:Glycosylation; D017261:Glycosylphosphatidylinositols / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D060847:Metabolic Engineering / Q000379:methods*; D009844:Oligosaccharides / Q000737:chemistry*; D010963:Plasmodium falciparum / Q000737:chemistry* / Q000472:pathogenicity; D013194:Staining and Labeling
chemical_list: D005456:Fluorescent Dyes; D006017:Glycolipids; D006028:Glycosphingolipids; D017261:Glycosylphosphatidylinositols; D009844:Oligosaccharides