title: Comparative proteomic profiling of mammalian cell lysates using phosphopeptide microarrays.
abstract: A library of 176 human phosphotyrosine-containing peptides was used to establish cell lysate binding profiles in a two colour microarray format. The resulting hits led to the pull-down and identification of biomarkers associated with cancer states.
authors.affiliation: Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543.
authors.forename: Liqian
authors.initials: L
authors.lastname: Gao
authors.forename: Mahesh
authors.initials: M
authors.lastname: Uttamchandani
authors.forename: Shao Q
authors.initials: SQ
authors.lastname: Yao
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis; D002460:Cell Line; D045744:Cell Line, Tumor; D006801:Humans; D019151:Peptide Library; D010748:Phosphopeptides / Q000737:chemistry*; D019000:Phosphotyrosine / Q000032:analysis*; D040081:Protein Array Analysis / Q000379:methods*; D040901:Proteomics / Q000379:methods*
chemical_list: D014408:Biomarkers, Tumor; D019151:Peptide Library; D010748:Phosphopeptides; D019000:Phosphotyrosine