title: High expression of Mfn1 promotes early development of bovine SCNT embryos: improvement of mitochondrial membrane potential and oxidative metabolism.
abstract: Mitofusin 1 (Mfn1) is the main mediator of mitochondrial fusion and homeostasis. To determine whether increased Mfn1 expression level could promote the fusion of heteroplasmic mitochondria and development of somatic cell nuclear transfer (SCNT) embryos. Embryos were constructed using bovine oocytes as recipient cytoplasm, and Holstein cow fetal fibroblasts with different expression levels of Mfn1 gene as donor nuclei. Mitochondrial membrane potential, ATP and H(2)O(2) generation, as well as the expression level of Mfn1 were detected in different development stages. The results showed that high level of Mfn1 expression significantly improved the embryo development rates by increasing ATP level and Δψm, while reducing H(2)O(2) generation. This study suggests that overexpression of Mfn1 could promote the early development of bovine SCNT embryos via improving oxidative phosphorylation.
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi Province, People's Republic of China.
authors.forename: Song
authors.initials: S
authors.lastname: Hua
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Yakun
authors.initials: Y
authors.lastname: Song
authors.forename: Ruizhe
authors.initials: R
authors.lastname: Li
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Wang
authors.forename: Fusheng
authors.initials: F
authors.lastname: Quan
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002417:Cattle / Q000254:growth & development*; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000502:physiology; D020558:GTP Phosphohydrolases / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D006861:Hydrogen Peroxide / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial; D008928:Mitochondria / Q000502:physiology*; D033681:Mitochondrial Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000502:physiology
chemical_list: D033681:Mitochondrial Membrane Transport Proteins; D000255:Adenosine Triphosphate; D006861:Hydrogen Peroxide; D020558:GTP Phosphohydrolases