title: Cleavage of pyrene-stabilized RNA bulge loops by trans-(±)-cyclohexane-1,2-diamine.
abstract: Chemical agents that cleave HIV genome can be potentially used for anti-HIV therapy. In this report, the cleavage of the upper stem-loop region of HIV-1 TAR RNA was studied in a variety of buffers containing organic catalysts. trans-(±)-Cyclohexane-1,2-diamine was found to cleave the RNA with the highest activity (31%, 37°C, 18 h). Cleavage of the RNA in trans-(±)-cyclohexane-1,2-diamine buffer was also studied when the RNA was hybridized with complementary DNAs. A pyrene-modified C3 spacer was incorporated to the DNA strand to facilitate the formation of a RNA bulge loop in the RNA/DNA duplex. In contrast, unmodified DNAs cannot efficiently generate RNA bulge loops, regardless of the DNA sequences. The results showed that the pyrene-stablized RNA bulge loops were efficiently and site-specifically cleaved by trans-(±)-cyclohexane-1,2-diamine.
authors.affiliation: Department of Chemistry and Environmental Science, New Jersey Institute of Technology, 323 Martin L, King Blvd, Newark, NJ, 07102, USA. haidong.huang@njit.edu.
authors.forename: Sejal
authors.initials: S
authors.lastname: Patel
authors.forename: Jagruti
authors.initials: J
authors.lastname: Rana
authors.forename: Jyoti
authors.initials: J
authors.lastname: Roy
authors.forename: Haidong
authors.initials: H
authors.lastname: Huang