title: Immune modulation by Lacto-N-fucopentaose III in experimental autoimmune encephalomyelitis.
abstract: Parasitic infections frequently lead to immune deviation or suppression. However, the application of specific parasitic molecules in regulating autoimmune responses remains to be explored. Here we report on the immune modulatory function of Lacto-N-fucopentaose III (LNFPIII), a schistosome glycan, in an animal model for multiple sclerosis. We found that LNFPIII treatment significantly reduced the severity of experimental autoimmune encephalomyelitis (EAE) and CNS inflammation, and skewed peripheral immune response to a Th2 dominant profile. Inflammatory monocytes (IMCs) purified from LNFPIII-treated mice had increased expression of nitric oxide synthase 2, and mediated T cell suppression. LNFPIII treatment also significantly increased mRNA expression of arginase-1, aldehyde dehydrogenase 1 subfamily A2, indoleamine 2,3-dioxygenase and heme oxygenase 1 in splenic IMCs. Furthermore, LNFPIII treatment significantly reduced trafficking of dendritic cells across brain endothelium in vitro. In summary, our study demonstrates that LNFPIII glycan treatment suppresses EAE by modulating both innate and T cell immune response.
authors.affiliation: Center for Neurologic Diseases, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Bing
authors.initials: B
authors.lastname: Zhu
authors.forename: Subbulaxmi
authors.initials: S
authors.lastname: Trikudanathan
authors.forename: Alla L
authors.initials: AL
authors.lastname: Zozulya
authors.forename: Carolina
authors.initials: C
authors.lastname: Sandoval-Garcia
authors.forename: Jennifer K
authors.initials: JK
authors.lastname: Kennedy
authors.forename: Olga
authors.initials: O
authors.lastname: Atochina
authors.forename: Thomas
authors.initials: T
authors.lastname: Norberg
authors.forename: Bastien
authors.initials: B
authors.lastname: Castagner
authors.forename: Peter
authors.initials: P
authors.lastname: Seeberger
authors.forename: Zsuzsa
authors.initials: Z
authors.lastname: Fabry
authors.forename: Donald
authors.initials: D
authors.lastname: Harn
authors.forename: Samia J
authors.initials: SJ
authors.lastname: Khoury
authors.forename: Indira
authors.initials: I
authors.lastname: Guleria
mesh_terms: D000606:Amino Sugars / Q000627:therapeutic use*; D000818:Animals; D002465:Cell Movement; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000188:drug therapy* / Q000276:immunology* / Q000473:pathology; D005260:Female; D007113:Immunity, Innate; D051379:Mice; D008810:Mice, Inbred C57BL; D009569:Nitric Oxide / Q000096:biosynthesis / Q000276:immunology; D010641:Phenotype; D011134:Polysaccharides / Q000627:therapeutic use*; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D000606:Amino Sugars; D011134:Polysaccharides; C434666:lacto-N-fucopentaose III; D009569:Nitric Oxide