title: [Establishment of molecular markers linked to sex in Schisandra sphenanthera].
abstract: OBJECTIVE
  To establish the gender-related RAPD markers in Schisandra sphenanthera.
  
  
  METHODS
  The genomic DNA was extracted from the young leaves of male and female Schisandra sphenanthera by modified CTAB method. The gender differences in the genome were studied by RAPD which was optimized by the single factor and orthogonal experiments.
  
  
  RESULTS
  25 microL total volume included Mg2+ of 2.5 mmol/L, dNTPs of 0.08 mmol/L, primer of 0.6 micromol/L, Taq enzyme 1.5 U, DNA template 60 ng, annealing temperature 41.3 degrees C, 35 cycles. In 400 random primers, only a male specific band 541 bp was generated by S353.
  
  
  CONCLUSIONS
  The marker can be used as the basis of gender identification.
authors.affiliation: Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, Xi'an 710062, China. bear880829@163.com
authors.forename: Yu-Ting
authors.initials: YT
authors.lastname: Xiong
authors.forename: Xin-Sheng
authors.initials: XS
authors.lastname: Wang
authors.forename: Ya-Xiang
authors.initials: YX
authors.lastname: Chen
authors.forename: Wen-Hui
authors.initials: WH
authors.lastname: Zong
authors.forename: Xiao
authors.initials: X
authors.lastname: Yang
authors.forename: Wei
authors.initials: W
authors.lastname: Gu
mesh_terms: D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics*; D008040:Genetic Linkage; D005819:Genetic Markers / Q000235:genetics*; D018745:Genome, Plant; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D019105:Random Amplified Polymorphic DNA Technique; D015203:Reproducibility of Results; D032141:Schisandra / Q000235:genetics*; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers