title: Nonstructural protein NS1 immunodominant epitope detected specifically in dengue virus infected material by a SELDI-TOF/MS based assay.
abstract: Dengue virus (DV) infection is the most common mosquito-born viral disease of public health significance. Though most patients only suffer from flu-like symptoms, a small group of patients experiences more severe forms of the disease. The viral nonstructural protein 1 (NS1), a secreted protein correlating with viremia, is a key element used for dengue diagnosis with potential implications in severe dengue prognosis. Capture-ELISAs for the early detection of the NS1 protein in the sera during the acute febrile stage are commonly used in routine by diagnostic laboratories. In this study, the detection of NS1 protein in DV-infected material was assessed by an alternative method combining a single NS1-directed monoclonal antibody and the SELDI-TOF/MS technology. According to the epitope mapping, the antibodies used are mainly directed against an immuno-dominant peptide located on the C-terminal part of the protein. The NS1 SELDI-TOF assay is specific, has a sensitivity level close to capture-ELISAs and is potentially useful for a coupled serotyping/detection assay or for the detection of subtle post-translational modifications on the protein.
authors.affiliation: Biomarker Department, BioMerieux SA, Chemin de l'Orme, Marcy l'Etoile, France.
authors.forename: Marine
authors.initials: M
authors.lastname: Steidel
authors.forename: Romain
authors.initials: R
authors.lastname: Fragnoud
authors.forename: Michelle
authors.initials: M
authors.lastname: Guillotte
authors.forename: Céline
authors.initials: C
authors.lastname: Roesch
authors.forename: Sandrine
authors.initials: S
authors.lastname: Michel
authors.forename: Thomas
authors.initials: T
authors.lastname: Meunier
authors.forename: Glàucia
authors.initials: G
authors.lastname: Paranhos-Baccalà
authors.forename: Gaspard
authors.initials: G
authors.lastname: Gervasi
authors.forename: Frederic
authors.initials: F
authors.lastname: Bedin
mesh_terms: D000293:Adolescent; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D002522:Chlorocebus aethiops; D003715:Dengue / Q000175:diagnosis* / Q000276:immunology; D003716:Dengue Virus / Q000276:immunology*; D018604:Epitope Mapping; D005260:Female; D057809:HEK293 Cells; D006801:Humans; D016056:Immunodominant Epitopes / Q000737:chemistry / Q000276:immunology*; D007223:Infant; D008297:Male; D008969:Molecular Sequence Data; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014709:Vero Cells; D017361:Viral Nonstructural Proteins / Q000737:chemistry / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D016056:Immunodominant Epitopes; D017361:Viral Nonstructural Proteins