title: Antidiabetic and cytotoxicity screening of five medicinal plants used by traditional African health practitioners in the Nelson Mandela Metropole, South Africa.
abstract: Diabetes mellitus is a growing problem in South Africa and of concern to traditional African health practitioners in the Nelson Mandela Metropole, because they experience a high incidence of diabetic cases in their practices. A collaborative research project with these practitioners focused on the screening of Bulbine frutescens, Ornithogalum longibracteatum, Ruta graveolens, Tarchonanthus camphoratus and Tulbaghia violacea for antidiabetic and cytotoxic potential. In vitro glucose utilisation assays with Chang liver cells and C2C12 muscle cells, and growth inhibition assays with Chang liver cells were conducted. The aqueous extracts of Bulbine frutescens (143.5%), Ornithogalum longibracteatum (131.9%) and Tarchonanthus camphoratus (131.5%) showed significant increased glucose utilisation activity in Chang liver cells. The ethanol extracts of Ruta graveolens (136.9%) and Tulbaghia violacea (140.5%) produced the highest increase in glucose utilisation in C2C12 muscle cells. The ethanol extract of Bulbine frutescens produced the most pronounced growth inhibition (33.3%) on Chang liver cells. These findings highlight the potential for the use of traditional remedies in the future for the management of diabetes and it is recommended that combinations of these plants be tested in future.
authors.affiliation: Department of Pharmacy, Nelson Mandela Metropolitan University, Port Elizabeth, South Africa.
authors.forename: Mea
authors.initials: M
authors.lastname: van Huyssteen
authors.forename: Pieter J
authors.initials: PJ
authors.lastname: Milne
authors.forename: Eileen E
authors.initials: EE
authors.lastname: Campbell
authors.forename: Maryna
authors.initials: M
authors.lastname: van de Venter
mesh_terms: D000490:Allium; D019659:Asteraceae; D002478:Cells, Cultured; D003603:Cytotoxins / Q000494:pharmacology* / Q000627:therapeutic use; D003920:Diabetes Mellitus / Q000188:drug therapy*; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007004:Hypoglycemic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D019668:Liliaceae; D008099:Liver / Q000166:cytology; D016488:Medicine, African Traditional; D031442:Ornithogalum; D008517:Phytotherapy; D010936:Plant Extracts; D010946:Plants, Medicinal; D032105:Ruta; D013019:South Africa
chemical_list: D003603:Cytotoxins; D007004:Hypoglycemic Agents; D010936:Plant Extracts; D005947:Glucose