title: Cultivar-specific kinetics of gene induction during downy mildew early infection in grapevine.
abstract: The oomycete pathogen Plasmopara viticola (Berk. et Curt.) Berl. et de Toni is the causing agent of the destructive downy mildew disease in grapevine. Despite the advances towards elucidation of grapevine resistance mechanisms to downy mildew, increased knowledge of the biological and genetic components of the pathosystem is important to design suitable breeding strategies. Previously, a cDNA microarray approach was used to compare two Vitis vinifera genotypes Regent and Trincadeira (resistant and susceptible to downy mildew, respectively) in field conditions. The same cDNA microarray chip was used to confirm field-based results and to compare both genotypes under greenhouse conditions at 0, 6, and 12 h post-inoculation with P. viticola. Results show that when comparing both cultivars after pathogen inoculation, there is a preferential modulation of several defense, signaling, and metabolism associated transcripts in Regent. Early transcriptional changes are discussed in terms of genetic background and resistance mechanism. This study is the first to directly compare resistant and susceptible cultivars responses as early as 6 hpi with P. viticola, providing several candidate genes potentially related to the expression of resistance traits.
authors.affiliation: Plant Systems Biology Lab, Center of Biodiversity, Functional & Integrative Genomics, Science Faculty of Lisbon University, 1749-016 Lisbon, Portugal. aafigueiredo@fc.ul.pt
authors.forename: Andreia
authors.initials: A
authors.lastname: Figueiredo
authors.forename: Filipa
authors.initials: F
authors.lastname: Monteiro
authors.forename: Ana Margarida
authors.initials: AM
authors.lastname: Fortes
authors.forename: Martina
authors.initials: M
authors.lastname: Bonow-Rex
authors.forename: Eva
authors.initials: E
authors.lastname: Zyprian
authors.forename: Lisete
authors.initials: L
authors.lastname: Sousa
authors.forename: Maria Salomé
authors.initials: MS
authors.lastname: Pais
mesh_terms: D060467:Disease Resistance / Q000235:genetics; D015870:Gene Expression; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D054884:Host-Pathogen Interactions; D007700:Kinetics; D020411:Oligonucleotide Array Sequence Analysis; D009868:Oomycetes / Q000502:physiology*; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D017422:Sequence Analysis, DNA; D015533:Transcriptional Activation; D027843:Vitis / Q000235:genetics* / Q000276:immunology / Q000382:microbiology