title: Stevioside ameliorates high-fat diet-induced insulin resistance and adipose tissue inflammation by downregulating the NF-κB pathway.
abstract: Accumulating evidence suggests that adipose tissue is the main source of pro-inflammatory molecules that predispose individuals to insulin resistance. Stevioside (SVS) is a widely used sweetener with multiple beneficial effects for diabetic patients. In this study, we investigated the effect of SVS on insulin resistance and the pro-inflammatory state of adipose tissue in mice fed with a high-fat diet (HFD). Oral administration of SVS for 1month had no effect on body weight, but it significantly improved fasting glucose, basal insulin levels, glucose tolerance and whole body insulin sensitivity. Interestingly, these changes were accompanied with decreased expression levels of several inflammatory cytokines in adipose tissue, including TNF-α, IL6, IL10, IL1β, KC, MIP-1α, CD11b and CD14. Moreover, macrophage infiltration in adipose tissue was remarkably reduced by SVS. Finally, SVS significantly suppressed the nuclear factor-kappa b (NF-κB) signaling pathway in adipose tissue. Collectively, these results suggested that SVS may ameliorate insulin resistance in HFD-fed mice by attenuating adipose tissue inflammation and inhibiting the NF-κB pathway.
authors.affiliation: State Key Laboratory of Medical Genomics, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
authors.forename: Zhiquan
authors.initials: Z
authors.lastname: Wang
authors.forename: Liqiong
authors.initials: L
authors.lastname: Xue
authors.forename: Cuicui
authors.initials: C
authors.lastname: Guo
authors.forename: Bing
authors.initials: B
authors.lastname: Han
authors.forename: Chunming
authors.initials: C
authors.lastname: Pan
authors.forename: Shuangxia
authors.initials: S
authors.lastname: Zhao
authors.forename: Huaidong
authors.initials: H
authors.lastname: Song
authors.forename: Qinyun
authors.initials: Q
authors.lastname: Ma
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects* / Q000473:pathology; D000818:Animals; D016207:Cytokines / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D059305:Diet, High-Fat / Q000009:adverse effects*; D045786:Diterpenes, Kaurane / Q000008:administration & dosage*; D015536:Down-Regulation; D005951:Glucose Tolerance Test; D005960:Glucosides / Q000008:administration & dosage*; D007249:Inflammation / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D007328:Insulin / Q000494:pharmacology; D007333:Insulin Resistance; D008264:Macrophages / Q000187:drug effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000378:metabolism; D012333:RNA, Messenger / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D013549:Sweetening Agents / Q000008:administration & dosage*
chemical_list: D016207:Cytokines; D045786:Diterpenes, Kaurane; D005960:Glucosides; D007328:Insulin; D016328:NF-kappa B; D012333:RNA, Messenger; D013549:Sweetening Agents; C012043:stevioside