title: The transcriptome of the adenovirus infected cell.
abstract: Alternations of cellular gene expression following an adenovirus type 2 infection of human primary cells were studied by using superior sensitive cDNA sequencing. In total, 3791 cellular genes were identified as differentially expressed more than 2-fold. Genes involved in DNA replication, RNA transcription and cell cycle regulation were very abundant among the up-regulated genes. On the other hand, genes involved in various signaling pathways including TGF-β, Rho, G-protein, Map kinase, STAT and NF-κB stood out among the down-regulated genes. Binding sites for E2F, ATF/CREB and AP2 were prevalent in the up-regulated genes, whereas binding sites for SRF and NF-κB were dominant among the down-regulated genes. It is evident that the adenovirus has gained a control of the host cell cycle, growth, immune response and apoptosis at 24 h after infection. However, efforts from host cell to block the cell cycle progression and activate an antiviral response were also observed.
authors.affiliation: Department of Immunology, Genetics and Pathology, Rudbeck Laboratory, Uppsala University, 751 85 Uppsala, Sweden. hongxing.zhao@igp.uu.se
authors.forename: Hongxing
authors.initials: H
authors.lastname: Zhao
authors.forename: Martin
authors.initials: M
authors.lastname: Dahlö
authors.forename: Anders
authors.initials: A
authors.lastname: Isaksson
authors.forename: Ann-Christine
authors.initials: AC
authors.lastname: Syvänen
authors.forename: Ulf
authors.initials: U
authors.lastname: Pettersson
mesh_terms: D000258:Adenovirus Infections, Human / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D000260:Adenoviruses, Human / Q000502:physiology*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism / Q000821:virology; D020869:Gene Expression Profiling; D006801:Humans; D008168:Lung / Q000166:cytology / Q000378:metabolism / Q000821:virology; D015398:Signal Transduction; D059467:Transcriptome