title: Evaluation of adding a second marker to overcome Staphylococcus aureus spa typing homoplasies.
abstract: The utility of sequencing a second highly variable locus in addition to the spa gene (e.g., double-locus sequence typing [DLST]) was investigated to overcome limitations of a Staphylococcus aureus single-locus typing method. Although adding a second locus seemed to increase discriminatory power, it was not sufficient to definitively infer evolutionary relationships within a single multilocus sequence type (ST-5).
authors.affiliation: Hospital Preventive Medicine Service, Centre Hospitalier Universitaire Vaudois and University of Lausanne, Lausanne, Switzerland. Patrick.Basset@chuv.ch
authors.forename: P
authors.initials: P
authors.lastname: Basset
authors.forename: U
authors.initials: U
authors.lastname: Nübel
authors.forename: W
authors.initials: W
authors.lastname: Witte
authors.forename: D S
authors.initials: DS
authors.lastname: Blanc
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics; D000942:Antigens, Bacterial / Q000235:genetics; D001499:Bayes Theorem; D019143:Evolution, Molecular; D005819:Genetic Markers; D006239:Haplotypes; D008390:Markov Chains; D008957:Models, Genetic; D009010:Monte Carlo Method; D058885:Multilocus Sequence Typing; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide; D013211:Staphylococcus aureus / Q000145:classification / Q000235:genetics*
chemical_list: D018829:Adhesins, Bacterial; D000942:Antigens, Bacterial; D005819:Genetic Markers; C434058:clfB protein, Staphylococcus aureus; C119204:streptococcal protective antigen