title: Modification of intracellular level of free radicals and apoptosis in cultured human endotheliocytes and carcinoma cells.
abstract: The intracellular levels of superoxide O(2)(-)and nitric oxide NO were directly measured under intravital conditions in cultured human endotheliocytes ECV 304 and carcinoma cells HeLa G-63. Comparative analysis of changes in the intracellular levels of superoxide and NO induced by ascorbic acid revealed a negative correlation between NO and O(2)(-)levels, whose strength depended on concentration of the acid. In pharmacological concentrations, ascorbic acid induced apoptotic death of carcinoma cells, but did not trigger apoptosis of endotheliocytes. The study demonstrated a possible way of cytotoxic action of ascorbic acid in pharmacological concentrations.
authors.affiliation: B. P. Konstantinov Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Russia. giliano@omrb.pnpi.spb.ru
authors.forename: N Ya
authors.initials: NY
authors.lastname: Giliano
authors.forename: L V
authors.initials: LV
authors.lastname: Konevega
authors.forename: L A
authors.initials: LA
authors.lastname: Noskin
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D001205:Ascorbic Acid / Q000378:metabolism* / Q000494:pharmacology; D045744:Cell Line, Tumor; D042783:Endothelial Cells / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D009569:Nitric Oxide / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D013481:Superoxides; D009569:Nitric Oxide; D001205:Ascorbic Acid