title: Lateral diffusion in equimolar mixtures of natural sphingomyelins with dioleoylphosphatidylcholine.
abstract: Cellular membranes of mammals are composed of a complex assembly of diverse phospholipids. Sphingomyelin (SM) and phosphatidylcholine (PC) are important lipids of eukaryotic cellular membranes and neuronal tissues, and presumably participate in the formation of membrane domains, known as "rafts," through intermolecular interaction and lateral microphase decomposition. In these two-dimensional membrane systems, lateral diffusion of lipids is an essential dynamic factor, which might even be indicative of lipid phase separation process. Here, we used pulsed field gradient nuclear magnetic resonance to study lateral diffusion of lipid components in macroscopically oriented bilayers composed of equimolar mixtures of natural SMs of egg yolk, bovine brain, bovine milk and dipalmitoylphosphatidylcholine (DPPC) with dioleoylphosphatidylcholine (DOPC). In addition, differential scanning calorimetry was used as a complementary technique to characterize the phase state of the lipid bilayers. In fully liquid bilayers, the lateral diffusion coefficients in both DOPC/DPPC and DOPC/SM systems exhibit mean values of the pure bilayers. For DOPC/SM bilayer system, this behavior can be explained by a model where most SM molecules form short-lived lateral domains with preferential SM-SM interactions occurring within them. However, for bilayers in the presence of their low-temperature gel phase, lateral diffusion becomes complicated and cannot simply be understood solely by a simple change in the liquid phase decomposition.
authors.affiliation: Kazan (Volga Region) Federal University, 420008 Kazan, Russian Federation. andrey.filippov@ksu.ru
authors.forename: Andrey
authors.initials: A
authors.lastname: Filippov
authors.forename: Bulat
authors.initials: B
authors.lastname: Munavirov
authors.forename: Gerhard
authors.initials: G
authors.lastname: Gröbner
authors.forename: Maya
authors.initials: M
authors.lastname: Rudakova
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry*; D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002645:Chickens; D004058:Diffusion; D004530:Egg Yolk / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008560:Membrane Fluidity; D021962:Membrane Microdomains; D008892:Milk / Q000737:chemistry; D008968:Molecular Conformation; D015394:Molecular Structure; D044367:Phase Transition; D013109:Sphingomyelins / Q000737:chemistry*
chemical_list: D008051:Lipid Bilayers; D013109:Sphingomyelins; D015060:1,2-Dipalmitoylphosphatidylcholine