title: Comparison of milk fat globule membrane (MFGM) proteins of Chianina and Holstein cattle breed milk samples through proteomics methods.
abstract: Identification of proteins involved in milk production is important to understand the biology of lactation. Many studies have advanced the understanding of mammary function and milk secretion, but the critical molecular mechanisms implicated in milk fat secretion is still incomplete. Milk fat globules are secreted from the apical surface of the mammary cells, surrounded by a thin membrane bilayer, the milk fat globule membrane (MFGM), formed by proteins which have been suggested to be cholesterolemia-lowering factors, inhibitors of cancer cell growth, vitamin binders, bactericidal, suppressors of multiple sclerosis. Using a proteomic approach, we compared MFGM from milk samples of individuals belonging to two different cattle breeds, Chianina and Holstein, representative of selection for milk and meat traits, respectively. We were able to isolate some of the major MFGM proteins in the examined samples and to identify differences between the protein fractions of the two breeds. We detected differences in the amount of proteins linked to mammary gland development and lipid droplets formation, as well as host defence mechanisms. We have shown that proteomics is a suitable, unbiased method for the study of milk fractions proteins and a powerful tool in nutritional genomics.
authors.affiliation: Dipartimento Produzioni Animali, Università degli Studi della Tuscia, Viterbo, 01100, Italy. lmurgiano@gmail.com
authors.forename: Leonardo
authors.initials: L
authors.lastname: Murgiano
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Timperio
authors.forename: Lello
authors.initials: L
authors.lastname: Zolla
authors.forename: Silvia
authors.initials: S
authors.lastname: Bongiorni
authors.forename: Alessio
authors.initials: A
authors.lastname: Valentini
authors.forename: Lorraine
authors.initials: L
authors.lastname: Pariset
mesh_terms: D000818:Animals; D002417:Cattle / Q000235:genetics* / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology; D006017:Glycolipids / Q000737:chemistry* / Q000378:metabolism; D006023:Glycoproteins / Q000737:chemistry* / Q000378:metabolism; D007774:Lactation / Q000502:physiology; D066292:Lipid Droplets; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics
chemical_list: D006017:Glycolipids; D006023:Glycoproteins; D008565:Membrane Proteins; C110367:milk fat globule