title: Role of thioproline on seed germination: interaction ROS-ABA and effects on antioxidative metabolism.
abstract: In this work we investigate the effect of the imbibition of pea seeds with different thioproline (TP) concentrations on the germination percentage and the early growth of the seedlings. The interaction between TP and hydrogen peroxide (H₂O₂) treatments is also analysed in order to test if any synergy in germination and growth occurs. Although the imbibition of pea seeds in the presence of TP did not significantly improve the germination percentage, TP and/or H₂O₂ pre-treatments increased seedlings growth. This increase in seedling growth was reduced by abscisic acid (ABA) addition. Imbibition of pea seeds in the presence of ABA also reduced the endogenous H₂O₂ contents of pea seedlings in control and TP-treated seeds. The incubation of pea seeds with TP and/or H₂O₂ in presence or absence of ABA decreased the activity of H₂O₂-scavenging enzymes. The increase of the endogenous H₂O₂ contents observed in TP and/or H₂O₂ treatments in absence of ABA could be correlated with the decrease in these activities. Finally, the hormone profile of pea seedlings was investigated. The results show that the increase in seedling growth is correlated with a decrease in ABA in samples pre-treated with H₂O₂ and TP + H₂O₂. Nevertheless, no significant differences in endogenous ABA concentration were observed with the TP pre-treatment. This paper suggests a relationship between endogenous H₂O₂ contents and plant growth, so reinforcing the intricate crosstalk between reactive oxygen species (ROS) and plant hormones in seed germination signalling and early seedling development.
authors.affiliation: Grupo de Biotecnología de Frutales-Dept. Mejora Vegetal, CEBAS-CSIC, Campus Universitario Espinardo, Apartado de Correos P.O. Box 164, E-30100 Murcia, Spain.
authors.forename: Gregorio
authors.initials: G
authors.lastname: Barba-Espin
authors.forename: Eduardo
authors.initials: E
authors.lastname: Nicolas
authors.forename: Maria Soledad
authors.initials: MS
authors.lastname: Almansa
authors.forename: Elena
authors.initials: E
authors.lastname: Cantero-Navarro
authors.forename: Alfonso
authors.initials: A
authors.lastname: Albacete
authors.forename: José Antonio
authors.initials: JA
authors.lastname: Hernández
authors.forename: Pedro
authors.initials: P
authors.lastname: Díaz-Vivancos
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D004357:Drug Synergism; D018525:Germination / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D018532:Pisum sativum / Q000187:drug effects* / Q000254:growth & development; D010937:Plant Growth Regulators / Q000494:pharmacology*; D036226:Seedlings / Q000187:drug effects / Q000254:growth & development; D012639:Seeds / Q000187:drug effects / Q000254:growth & development; D053778:Thiazolidines / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D010937:Plant Growth Regulators; D053778:Thiazolidines; D000040:Abscisic Acid; D006861:Hydrogen Peroxide; C003438:thiazolidine-4-carboxylic acid