title: Tudor-SN and ADAR1 are components of cytoplasmic stress granules.
abstract: Hyperediting by adenosine deaminases that acts on RNA (ADARs) may result in numerous Adenosine-to-Inosine (A-to-I) substitutions within long dsRNA. However, while countless RNAs may undergo hyperediting, the role for inosine-containing hyperedited dsRNA (IU-dsRNA) in cells is poorly understood. We have previously shown that IU-dsRNA binds specifically to various components of cytoplasmic stress granules, as well as to other proteins such as Tudor Staphylococcal Nuclease (Tudor-SN). Tudor-SN has been implicated in diverse roles in mammalian cells, including transcription, splicing, RNAi, and degradation. Moreover, we have shown that Tudor-SN interacts directly with stress granule proteins. Here we show that Tudor-SN localizes to cytoplasmic stress granules in HeLa cells undergoing arsenite-induced oxidative stress, or following transfection with long dsRNA (poly[IC]), which initiates an interferon cascade. We additionally demonstrate a novel interaction between Tudor-SN and ADAR1. Finally, we show that ADAR1 is also localized to stress granules in HeLa cells following various stresses.
authors.affiliation: Department of Biochemistry, University of Cambridge, Cambridge CB2 1QW, United Kingdom.
authors.forename: Rebekka
authors.initials: R
authors.lastname: Weissbach
authors.forename: A D J
authors.initials: AD
authors.lastname: Scadden
mesh_terms: D000243:Adenosine Deaminase / Q000235:genetics / Q000378:metabolism*; D018053:Arsenites / Q000494:pharmacology; D045744:Cell Line, Tumor; D003594:Cytoplasmic Granules / Q000737:chemistry* / Q000378:metabolism*; D004720:Endonucleases; D006367:HeLa Cells; D006801:Humans; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D011070:Poly I-C / Q000494:pharmacology; D011485:Protein Binding / Q000502:physiology; D017434:Protein Structure, Tertiary; D021381:Protein Transport / Q000187:drug effects; D016601:RNA-Binding Proteins; D013312:Stress, Physiological; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors
chemical_list: D018053:Arsenites; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins; D014969:Xanthine Oxidase; D004720:Endonucleases; C095221:SND1 protein, human; C567955:ADARB1 protein, human; D000243:Adenosine Deaminase; C015001:arsenite; D011070:Poly I-C