title: Lung cancer alters the hydrolysis of nucleotides and nucleosides in platelets.
abstract: BACKGROUND
  The nucleotides and nucleosides of adenine are signaling molecules related to thromboregulation and modulation of immune responses in patients with malignancies. Thus, this study aims to determine NTPDase, 5'-nucleotidase, ectonucleotide pyrophosphatase/phosphodiesterase (E-NPP) and adenosine deaminase (ADA) activities in the platelets of patients with lung cancer.
  
  
  METHODS
  We collected blood samples from patients (n=33) previously treated for lung cancer with chemotherapy. Patients were classified as stage IIIb and IV according to the Union for International Cancer Control (UICC).
  
  
  RESULTS
  Patients showed a significant decrease in the hydrolysis of adenosine diphosphate (ADP) and adenosine, whereas the adenosine monophosphate (AMP) hydrolysis and platelet aggregation were significantly increased in this group. Adenosine triphosphate (ATP) hydrolysis did not show significant results between the group of patients and the control group.
  
  
  CONCLUSIONS
  We may suggest that ectonucleotidases as well as ADA are enzymes involved in thromboembolic events but especially here we may see that they are also directly involved in the generation of adenosine formation in the cancer patient circulation.
authors.affiliation: Centro de Ciências Naturais e Exatas, Departamento de Química, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900 Santa Maria, RS, Brazil.
authors.forename: Daniela
authors.initials: D
authors.lastname: Zanini
authors.forename: Roberta
authors.initials: R
authors.lastname: Schmatz
authors.forename: Victor Camera
authors.initials: VC
authors.lastname: Pimentel
authors.forename: Jessié Martins
authors.initials: JM
authors.lastname: Gutierres
authors.forename: Paula Acosta
authors.initials: PA
authors.lastname: Maldonado
authors.forename: Gustavo Roberto
authors.initials: GR
authors.lastname: Thomé
authors.forename: Andréia Machado
authors.initials: AM
authors.lastname: Cardoso
authors.forename: Naiara
authors.initials: N
authors.lastname: Stefanello
authors.forename: Liliane
authors.initials: L
authors.lastname: Oliveira
authors.forename: Juarez
authors.initials: J
authors.lastname: Chiesa
authors.forename: Daniela Bitencourt Rosa
authors.initials: DB
authors.lastname: Leal
authors.forename: Vera Maria
authors.initials: VM
authors.lastname: Morsch
authors.forename: Maria Rosa Chitolina
authors.initials: MR
authors.lastname: Schetinger
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000970:Antineoplastic Agents / Q000627:therapeutic use; D001792:Blood Platelets / Q000378:metabolism; D002289:Carcinoma, Non-Small-Cell Lung / Q000188:drug therapy / Q000473:pathology*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D006868:Hydrolysis; D008175:Lung Neoplasms / Q000188:drug therapy / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D009705:Nucleosides / Q000378:metabolism*; D009711:Nucleotides / Q000378:metabolism*; D010974:Platelet Aggregation
chemical_list: D000970:Antineoplastic Agents; D009705:Nucleosides; D009711:Nucleotides