title: [Identification of the metabolites of Sinisan extract in rat plasma, urine, feces and bile after intragastric administration].
abstract: Sinisan is a widely used traditional Chinese medicine (TCM) in treating various diseases; however, the in vivo metabolic profile of its multiple components remains unknown. In this paper, ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) was applied to identify the metabolites of Sinisan extract in rat plasma, urine, feces and bile after intragastric administration. Using MS(E) and mass defect filter techniques, 41 metabolites of 10 parent compounds (naringin, naringenin, hesperidin, neohesperidin, liquiritin, liquiritigenin, glycyrrhizic acid, glycyrrhetinic acid, saikosaponin a and saikosaponin d) were detected and tentatively identified. It was shown by our results that these compounds was metabolized to the forms of hydroxylation, glucuronidation, sulfation, glucuronidation with sulfation and glucuronidation with hydroxylation in vivo.
authors.affiliation: Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing 210046, China.
authors.forename: Lin-ling
authors.initials: LL
authors.lastname: Lu
authors.forename: Yan
authors.initials: Y
authors.lastname: Shu
authors.forename: Da-wei
authors.initials: DW
authors.lastname: Qian
authors.forename: Shu-lan
authors.initials: SL
authors.lastname: Su
authors.forename: Jin-ao
authors.initials: JA
authors.lastname: Duan
authors.forename: Ye-fei
authors.initials: YF
authors.lastname: Qian
authors.forename: Cai-fu
authors.initials: CF
authors.lastname: Xue
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000378:metabolism* / Q000493:pharmacokinetics*; D044950:Flavanones / Q000032:analysis / Q000378:metabolism / Q000493:pharmacokinetics; D005960:Glucosides / Q000032:analysis / Q000378:metabolism / Q000493:pharmacokinetics; D019695:Glycyrrhizic Acid / Q000032:analysis / Q000378:metabolism / Q000493:pharmacokinetics; D006569:Hesperidin / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism / Q000493:pharmacokinetics; D006900:Hydroxylation; D008297:Male; D010946:Plants, Medicinal / Q000737:chemistry*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D004365:Drugs, Chinese Herbal; D044950:Flavanones; D005960:Glucosides; D019695:Glycyrrhizic Acid; D006569:Hesperidin; C005273:naringenin; C005274:naringin; C546526:neohesperidin; C512196:liquiritin; C083152:liquiritigenin