title: FOXP3(+)  regulatory and TIA-1(+)  cytotoxic T lymphocytes in HIV-associated Hodgkin lymphoma.
abstract: Human immunodeficiency virus (HIV) infects CD4(+)  lymphocytes, leading to a development of malignant lymphomas, such as HIV-associated Hodgkin Lymphoma (HIV-HL). This study aimed to assess the differences in cellular composition of the inflammatory reactive background of HIV-HLs. We examined infiltrating T lymphocytes, specifically regulatory T cells, cytotoxic cells, Epstein-Barr virus (EBV) related antigens and HIV-receptor CCR5. In all HIV-HL cases, Hodgkin and Reed-Sternberg (HRS) cells showed EBER1 expression, LMP-1 staining positivity and EBNA-2 staining negativity, except for one case which showed LMP-1 staining negativity. Our histological findings indicate the percentage of CD8(+) , TIA-1(+)  lymphocytes was significantly higher in HIV-HL than in non-HIV-HL cases (P < 0.05). On the other hand, the percentage of CD4(+) , FOXP3(+)  lymphocytes was significantly lower in HIV-HL than in non-HIV-HL cases (P < 0.05) but present. The percentage of CCR5(+)  lymphocytes was significantly lower in HIV-HL than in non-HIV-HL cases (P < 0.05). Usually, CD4(+)  and CCR5(+)  lymphocytes are reported to be rarely detected in HIV-associated non-Hodgkin lymphomas, but the presence of CD4(+)  and/or FOXP3(+)  lymphocytes may be implicated in the pathogenesis of HL. In addition, although additional CD8(+)  lymphocytes are probably not EBV-LMP specific cytotoxic T-cells, these lymphocytes may also well be involved in the pathogenesis of HIV-HL.
authors.affiliation: Department of Pathology, School of Medicine, Kurume University, Japan. jkiyasu@med.kyushu-u.ac.jp
authors.forename: Junichi
authors.initials: J
authors.lastname: Kiyasu
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Aoki
authors.forename: Paula Yurie
authors.initials: PY
authors.lastname: Tanaka
authors.forename: Luis Fernando
authors.initials: LF
authors.lastname: Pracchia
authors.forename: Edenilson Eduardo
authors.initials: EE
authors.lastname: Calore
authors.forename: Nilda Maria
authors.initials: NM
authors.lastname: Perez
authors.forename: Yoshizo
authors.initials: Y
authors.lastname: Kimura
authors.forename: Daisuke
authors.initials: D
authors.lastname: Niino
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Sugita
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Takayanagi
authors.forename: Yasunobu
authors.initials: Y
authors.lastname: Abe
authors.forename: Masao
authors.initials: M
authors.lastname: Matsuoka
authors.forename: Koichi
authors.initials: K
authors.lastname: Ohshima
mesh_terms: D000328:Adult; D051858:Forkhead Transcription Factors / Q000378:metabolism*; D006678:HIV / Q000276:immunology*; D015658:HIV Infections / Q000150:complications / Q000276:immunology* / Q000378:metabolism; D006689:Hodgkin Disease / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D006801:Humans; D008297:Male; D039102:Poly(A)-Binding Proteins / Q000378:metabolism*; D016539:Reed-Sternberg Cells / Q000276:immunology / Q000378:metabolism; D000074019:T-Cell Intracellular Antigen-1; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology / Q000378:metabolism*
chemical_list: C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D039102:Poly(A)-Binding Proteins; D000074019:T-Cell Intracellular Antigen-1; C071558:TIA1 protein, human