title: SNPs of hemocyanin C-terminal fragment in shrimp Litopenaeus vannamei.
abstract: In this study, we identified a variable region in the C-terminus of hemocyanin from the shrimp Litopenaeus vannamei (2288-2503bp, HcSC) by sequence alignments. A total of 13 SNPs were identified by PCR-SSCP and HcSC clone sequencing. The SSCP patterns of HcSC could be modulated in Vibro parahaemolyticus-treated shrimps. A novel SSCP band with four SNP sites was identified in V. parahaemolyticus-resistant shrimps. More importantly, three of these four SNPs introduced variations in amino acid sequence and possibly secondary structure of the HcSC polypeptide and resulted in a higher agglutinative activity against seven pathogenic bacteria. These results suggest that the C-terminus of shrimp L. vannamei hemocyanin possesses SNPs, which may be related to shrimp resistance to different pathogens.
authors.affiliation: Department of Biology and Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou, People's Republic of China.
authors.forename: Xianliang
authors.initials: X
authors.lastname: Zhao
authors.forename: Lingling
authors.initials: L
authors.lastname: Guo
authors.forename: Yueling
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yao
authors.initials: Y
authors.lastname: Liu
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Zhang
authors.forename: Jingsheng
authors.initials: J
authors.lastname: Lun
authors.forename: Jiehui
authors.initials: J
authors.lastname: Chen
authors.forename: Yuanyou
authors.initials: Y
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D006433:Hemocyanins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000737:chemistry / Q000235:genetics* / Q000382:microbiology; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D018807:Polymorphism, Single-Stranded Conformational; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013312:Stress, Physiological; D014735:Vibrio Infections / Q000235:genetics / Q000662:veterinary; D014736:Vibrio parahaemolyticus / Q000472:pathogenicity
chemical_list: D017931:DNA Primers; D010446:Peptide Fragments; D006433:Hemocyanins