title: The preoperative activity of Th1 and Th17 cytokine axes in prediction of sepsis after radical cystectomy.
abstract: The aim of the study was to correlate the preoperative activity of Th1 and Th17 cytokine axes with the development of sepsis after radical cystectomy. The study involved twenty patients with the infiltrative transitional cell carcinoma of the urinary bladder without previous radiotherapy/chemotherapy, who underwent open radical cystectomy with urinary diversion. Preoperative plasma concentrations of Th1 cytokines interleukin 12 (IL-12) and interferon gamma (IFN-γ), and Th17 cytokines IL-23 and IL-17, were measured using ELISA. Preoperative expression of mRNA for IL-12p35, IFN-γ, IL-23p19 and IL-17 was quantified by real-time RT-PCR using mRNA extracted from peripheral blood mononuclear cells. Eight patients developed postoperative sepsis, diagnosed within two weeks post-operation as systemic inflammatory response syndrome in the presence of local or systemic infection. The preoperative basal plasma concentrations of Th1 and Th17 cytokines were slightly above the detection limits, with a tendency toward lower concentrations in patients who developed sepsis, but the difference was not significant (p>0.05). The preoperative expression of mRNA encoding IL-12p35 and IL-17 was significantly lower in patients who developed sepsis (p=0.003 and p=0.028, respectively). The similar trend was observed for IL-23p19 and IFN-γ, but the differences did not reach the statistical significance (p=0.051 and p=0.172, respectively). These data suggest that determination of preoperative Th1 and Th17 cytokine mRNA levels might be useful in predicting sepsis development after radical cystectomy.
authors.affiliation: Institute of Urology and Nephrology, School of Medicine, University of Belgrade, Belgrade, Serbia.
authors.forename: Cane
authors.initials: C
authors.lastname: Tulic
authors.forename: Miodrag
authors.initials: M
authors.lastname: Lazic
authors.forename: Emina
authors.initials: E
authors.lastname: Savic
authors.forename: Dusan
authors.initials: D
authors.lastname: Popadic
authors.forename: Jelena
authors.initials: J
authors.lastname: Djukic
authors.forename: Dusan
authors.initials: D
authors.lastname: Spasic
authors.forename: Milos
authors.initials: M
authors.lastname: Markovic
authors.forename: Zorica
authors.initials: Z
authors.lastname: Ramic
authors.forename: Marija
authors.initials: M
authors.lastname: Mostarica-Stojkovic
authors.forename: Vladimir
authors.initials: V
authors.lastname: Trajkovic
mesh_terms: D000368:Aged; D002295:Carcinoma, Transitional Cell / Q000601:surgery; D015653:Cystectomy / Q000009:adverse effects*; D016207:Cytokines / Q000097:blood*; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000097:blood; D018664:Interleukin-12 / Q000097:blood; D020381:Interleukin-17 / Q000097:blood; D053759:Interleukin-23 / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000166:cytology; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000096:biosynthesis; D018805:Sepsis / Q000209:etiology*; D013530:Surgical Wound Infection; D018417:Th1 Cells / Q000276:immunology* / Q000378:metabolism; D058504:Th17 Cells / Q000276:immunology* / Q000378:metabolism; D001743:Urinary Bladder / Q000473:pathology; D001749:Urinary Bladder Neoplasms / Q000601:surgery*
chemical_list: D016207:Cytokines; D020381:Interleukin-17; D053759:Interleukin-23; D012333:RNA, Messenger; D018664:Interleukin-12; D007371:Interferon-gamma