title: An experimental approach towards the development of an in vitro cortical-thalamic co-culture model.
abstract: In this paper, we propose an experimental approach to develop an in vitro dissociated cortical-thalamic co-culture model using a dual compartment neurofluidic device. The device has two compartments separated by 10 μm wide and 3 μm high microchannels. The microchannels provide a physical isolation of neurons allowing only neurites to grow between the compartments. Long-term viable co-culture was maintained in the compartmented device, neurite growth through the microchannels was verified using immunofluorescence staining, and electrophysiological recordings from the co-culture system was investigated. Preliminary analysis of spontaneous activities from the co-culture shows a distinctively different firing pattern associated with cultures of individual cell types and further analysis is proposed for a deeper understanding of the dynamics involved in the network connectivity in such a co-culture system.
authors.affiliation: Department of Minimally Invasive Healthcare, Philips Research Laboratories Eindhoven, High TechCampus 34.2(023), 5656 AE Eindhoven, The Netherlands. thiru.kanagasabapathi@Philips.com
authors.forename: Thirukumaran T
authors.initials: TT
authors.lastname: Kanagasabapathi
authors.forename: Paolo
authors.initials: P
authors.lastname: Massobrio
authors.forename: Mariateresa
authors.initials: M
authors.lastname: Tedesco
authors.forename: Sergio
authors.initials: S
authors.lastname: Martinoia
authors.forename: Wytse J
authors.initials: WJ
authors.lastname: Wadman
authors.forename: Michel M J
authors.initials: MM
authors.lastname: Decré
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology* / Q000502:physiology*; D018920:Coculture Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D009434:Neural Pathways / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D013788:Thalamus / Q000166:cytology* / Q000502:physiology*