title: Optimal scaffold design and effective progenitor cell identification for the regeneration of vascularized bone.
abstract: Bone tissue engineering offers perhaps the most attractive treatment option for bone repair/regeneration as it eliminates complications of other bone grafting options (i.e., availability and immunogenicity issues of autografts and allografts, respectively). However, scaffold-based bone tissue engineering is largely limited by inadequate vascaularization, and as a result, bone formation is often restricted to the construct's periphery. In this study, we offer a two-pronged approach to overcome periphery-limited bone and vascular formation. We have developed optimally designed, mechanically strong, biodegradable scaffolds with increased porosity and interconnectivity. We have also identified and isolated superior, clinically-relevant cell populations (peripheral blood-derived endothelial progenitor cells (EPCs), and bone marrow-derived mesenchymal stem cells (MSCs)). In combination, we have developed a synthetic graft system suitable for the regeneration of vascularized bone.
authors.affiliation: Orthopaedic Surgery, University of Connecticut Health Center, Farmington, CT 06030, USA. syam@uchc.edu
authors.forename: Syam P
authors.initials: SP
authors.lastname: Nukavarapu
authors.forename: Ami R
authors.initials: AR
authors.lastname: Amini
mesh_terms: D001861:Bone Regeneration; D001842:Bone and Bones / Q000098:blood supply* / Q000166:cytology; D006801:Humans; D013234:Stem Cells / Q000166:cytology