title: Post-natal cardiomyocytes can generate iPS cells with an enhanced capacity toward cardiomyogenic re-differentation.
abstract: Adult mammalian cells can be reprogrammed to a pluripotent state by forcing the expression of a few embryonic transcription factors. The resulting induced pluripotent stem (iPS) cells can differentiate into cells of all three germ layers. It is well known that post-natal cardiomyocytes (CMs) lack the capacity to proliferate. Here, we report that neonatal CMs can be reprogrammed to generate iPS cells that express embryonic-specific markers and feature gene-expression profiles similar to those of mouse embryonic stem (mES) cell and cardiac fibroblast (CF)-derived iPS cell populations. CM-derived iPS cells are able to generate chimeric mice and, moreover, re-differentiate toward CMs more efficiently then either CF-derived iPS cells or mES cells. The increased differentiation capacity is possibly related to CM-derived iPS cells retaining an epigenetic memory of the phenotype of their founder cell. CM-derived iPS cells may thus lead to new information on differentiation processes underlying cardiac differentiation and proliferation.
authors.affiliation: Cell Biology and Neurobiology Institute, National Research Council of Italy, Roma, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Rizzi
authors.forename: E
authors.initials: E
authors.lastname: Di Pasquale
authors.forename: P
authors.initials: P
authors.lastname: Portararo
authors.forename: R
authors.initials: R
authors.lastname: Papait
authors.forename: P
authors.initials: P
authors.lastname: Cattaneo
authors.forename: M V G
authors.initials: MV
authors.lastname: Latronico
authors.forename: C
authors.initials: C
authors.lastname: Altomare
authors.forename: L
authors.initials: L
authors.lastname: Sala
authors.forename: A
authors.initials: A
authors.lastname: Zaza
authors.forename: E
authors.initials: E
authors.lastname: Hirsch
authors.forename: L
authors.initials: L
authors.lastname: Naldini
authors.forename: G
authors.initials: G
authors.lastname: Condorelli
authors.forename: C
authors.initials: C
authors.lastname: Bearzi
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D065150:Cellular Reprogramming; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D005786:Gene Expression Regulation; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D007621:Karyotyping; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors / Q000378:metabolism; D051379:Mice; D032383:Myocytes, Cardiac / Q000166:cytology*; D050814:Octamer Transcription Factor-3 / Q000378:metabolism; D055748:SOXB1 Transcription Factors / Q000378:metabolism
chemical_list: D055396:Bone Morphogenetic Protein 2; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors; D050814:Octamer Transcription Factor-3; D055748:SOXB1 Transcription Factors; D002118:Calcium