title: Evaluation of a new immunochromatographic assay kit for the rapid detection of norovirus in fecal specimens.
abstract: Rapid and accurate detection of norovirus is essential for the prevention and control of norovirus outbreaks. This study compared the effectiveness of a new immunochromatographic assay kit (SD BIOLINE Norovirus; Standard Diagnostics, Korea) and real-time reverse transcription-PCR (RT-PCR) for detecting norovirus in fecal specimens. Compared with real-time RT-PCR, the new assay had sensitivity, specificity, positive predictive value, and negative predictive value of 76.5% (52/68), 99.7% (342/343), 98.1% (52/53), and 95.5% (342/358), respectively. The sensitivity of the assay was 81.8% (18/22) for GII.3 and 75.7% (28/37) for GII.4. None of the 38 enteric virus-positive specimens (3 for astrovirus, 5 for enteric adenovirus, and 30 for rotavirus) tested positive in the cross-reactivity test performed by using this assay. The new immunochromatographic assay may be a useful screening tool for the rapid detection of norovirus in sporadic and outbreak cases; however, negative results may require confirmatory assays of greater sensitivity.
authors.affiliation: Chungcheongnam-Do Health and Environment Research Institute, Daejeon, Korea.
authors.forename: Kwi Sung
authors.initials: KS
authors.lastname: Park
authors.forename: Kyoung Ah
authors.initials: KA
authors.lastname: Baek
authors.forename: Dong Uk
authors.initials: DU
authors.lastname: Kim
authors.forename: Kyung Sook
authors.initials: KS
authors.lastname: Kwon
authors.forename: Sun Hye
authors.initials: SH
authors.lastname: Bing
authors.forename: Joon Soo
authors.initials: JS
authors.lastname: Park
authors.forename: Hae Seon
authors.initials: HS
authors.lastname: Nam
authors.forename: Sang Han
authors.initials: SH
authors.lastname: Lee
authors.forename: Young Jin
authors.initials: YJ
authors.lastname: Choi
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017250:Caliciviridae Infections / Q000175:diagnosis*; D002648:Child; D002675:Child, Preschool; D005243:Feces / Q000821:virology*; D005759:Gastroenteritis / Q000175:diagnosis* / Q000821:virology; D006801:Humans; D007118:Immunoassay; D007223:Infant; D008875:Middle Aged; D029322:Norovirus / Q000235:genetics* / Q000302:isolation & purification; D012367:RNA, Viral / Q000032:analysis; D011933:Reagent Kits, Diagnostic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic