title: Exfoliative cytology and cytocentrifuge preparation of oral premalignant and malignant lesions.
abstract: OBJECTIVE
  Oral exfoliative cytology is a sensitive and reliable diagnostic tool for the early diagnosis of oral cancer. Traditional exfoliative cytology, though popular, has limitations which could be overcome using cytocentrifuge preparations. The purpose of this study was to compare the efficacy of cytocentrifuged specimens with that of conventionally prepared smears in the diagnosis of oral premalignancy and malignancy.
  
  
  METHODS
  Exfoliative cytology was performed on 30 patients with oral premalignant and malignant lesions. The yield was smeared immediately onto a slide for conventional exfoliative cytology (group A). The lesion was then scraped again and the yield was suspended in a bottle containing a fixative solution for cytocentrifuge apparatus-assisted smearing (group B). Both smears were stained with rapid Papanicolaou stain and assessed for cellularity, cellular and nuclear morphology, evaluation of mitosis and background.
  
  
  RESULTS
  All the criteria assessed were statistically significant (p value of <0.001) in group B except for mitosis, which was significant in group A.
  
  
  CONCLUSIONS
  Cytocentrifuge preparations can preserve the cellular details and reduce the overlapping of cells, enabling precise interpretation and thus aiding in the diagnosis of premalignant and malignant lesions of the oral cavity.
authors.affiliation: Department of Oral Pathology and Microbiology, KLE VK Institute of Dental Sciences, Belgaum, Karnataka, India. drpushpakanu@yahoo.co.in
authors.forename: Pushpak
authors.initials: P
authors.lastname: Shah
authors.forename: Revati
authors.initials: R
authors.lastname: Deshmukh
mesh_terms: D000328:Adult; D000368:Aged; D002278:Carcinoma in Situ / Q000175:diagnosis*; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis*; D002452:Cell Count; D002467:Cell Nucleus / Q000473:pathology; D002498:Centrifugation; D003581:Cytodiagnosis / Q000379:methods; D042241:Early Diagnosis; D005260:Female; D006801:Humans; D007971:Leukoplakia / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D008938:Mitosis; D009062:Mouth Neoplasms / Q000175:diagnosis*; D013194:Staining and Labeling