title: In vitro targeting of avidin-expressing glioma cells with biotinylated persistent luminescence nanoparticles.
abstract: Far red emitting persistent luminescence nanoparticles (PLNP) were synthesized and functionalized with biotin to study their targeting ability toward biotin-binding proteins. First, the interaction of biotin-decorated PLNP with streptavidin, immobilized on a plate, was shown to be highly dependent on the presence of a PEG spacer between the surface of the nanoparticles and the biotin ligand. Second, interaction between biotin-PEG-PLNP and free neutravidin in solution was confirmed by fluorescence microscopy. Finally, in vitro binding study on BT4C cells expressing lodavin fusion protein, bearing the extracellular avidin moiety, showed that such biotin-covered PLNP could successfully be targeted to malignant glioma cells through a specific biotin-avidin interaction. The influence of nanoparticle core diameter, incubation time, and PLNP concentration on the efficiency of targeting is discussed.
authors.affiliation: Unité de Pharmacologie Chimique et Génétique et d'Imagerie, CNRS, UMR 8151, Inserm, U 1022, Université Paris Descartes, Paris, F-75270 Cedex, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Maldiney
authors.forename: Minna U
authors.initials: MU
authors.lastname: Kaikkonen
authors.forename: Johanne
authors.initials: J
authors.lastname: Seguin
authors.forename: Quentin
authors.initials: Q
authors.lastname: le Masne de Chermont
authors.forename: Michel
authors.initials: M
authors.lastname: Bessodes
authors.forename: Kari J
authors.initials: KJ
authors.lastname: Airenne
authors.forename: Seppo
authors.initials: S
authors.lastname: Ylä-Herttuala
authors.forename: Daniel
authors.initials: D
authors.lastname: Scherman
authors.forename: Cyrille
authors.initials: C
authors.lastname: Richard
mesh_terms: D000818:Animals; D001360:Avidin / Q000378:metabolism*; D001710:Biotin / Q000378:metabolism*; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D005455:Fluorescent Antibody Technique; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D066298:In Vitro Techniques; D049449:Luminescence; D008856:Microscopy, Fluorescence; D053758:Nanoparticles; D051381:Rats
chemical_list: D001360:Avidin; D001710:Biotin