title: Eight RGS and RGS-like proteins orchestrate growth, differentiation, and pathogenicity of Magnaporthe oryzae.
abstract: A previous study identified MoRgs1 as an RGS protein that negative regulates G-protein signaling to control developmental processes such as conidiation and appressorium formation in Magnaporthe oryzae. Here, we characterized additional seven RGS and RGS-like proteins (MoRgs2 through MoRgs8). We found that MoRgs1 and MoRgs4 positively regulate surface hydrophobicity, conidiation, and mating. Indifference to MoRgs1, MoRgs4 has a role in regulating laccase and peroxidase activities. MoRgs1, MoRgs2, MoRgs3, MoRgs4, MoRgs6, and MoRgs7 are important for germ tube growth and appressorium formation. Interestingly, MoRgs7 and MoRgs8 exhibit a unique domain structure in which the RGS domain is linked to a seven-transmembrane motif, a hallmark of G-protein coupled receptors (GPCRs). We have also shown that MoRgs1 regulates mating through negative regulation of Gα MoMagB and is involved in the maintenance of cell wall integrity. While all proteins appear to be involved in the control of intracellular cAMP levels, only MoRgs1, MoRgs3, MoRgs4, and MoRgs7 are required for full virulence. Taking together, in addition to MoRgs1 functions as a prominent RGS protein in M. oryzae, MoRgs4 and other RGS and RGS-like proteins are also involved in a complex process governing asexual/sexual development, appressorium formation, and pathogenicity.
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, and Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Tang
authors.forename: Kaiyue
authors.initials: K
authors.lastname: Liu
authors.forename: Qian
authors.initials: Q
authors.lastname: Huang
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Xia
authors.initials: X
authors.lastname: Yan
authors.forename: Yue
authors.initials: Y
authors.lastname: Chen
authors.forename: Jiansheng
authors.initials: J
authors.lastname: Wang
authors.forename: Zhongqiang
authors.initials: Z
authors.lastname: Qi
authors.forename: Zhengyi
authors.initials: Z
authors.lastname: Wang
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Zheng
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Zhengguang
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D002473:Cell Wall / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D020082:Magnaporthe / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity*; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology*; D020710:RGS Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D020710:RGS Proteins