title: Analysis of integrated optofluidic lab-on-a-chip fluorescence biosensor based on transmittance of light through a fluidic gap.
abstract: Sensitivity analysis is an important aspect to be looked into while designing lab-on-a-chip systems. In this paper we will be showing with appropriate design that the best sensitivity of the fluorescence biosensor is achieved for an optimal width of fluidic gap, corresponding to a particular mode spot size. We will be also showing that the sensitivity of the biosensor is affected by efficiency of light coupling, which is influenced by changes in the width of fluidic gap, refractive index of the fluid and higher order modes.
authors.affiliation: Department of Instrumentation and Applied Physics, Institute of Science, Bangalore 560012, India. narayank@airtelmail.in
authors.forename: Narayan
authors.initials: N
authors.lastname: Krishnaswamy
authors.forename: T
authors.initials: T
authors.lastname: Srinivas
authors.forename: G Mohan
authors.initials: GM
authors.lastname: Rao
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008027:Light; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D055096:Optical Devices; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*; D018511:Systems Integration