title: Key role of CXCL13/CXCR5 axis for cerebrospinal fluid B cell recruitment in pediatric OMS.
abstract: To study aberrant B cell trafficking into the CSF in opsoclonus-myoclonus syndrome (OMS), chemoattractants CXCL13 and CXCL12, and B cell frequency and CXCR5 expression, were evaluated. CSF CXCL13 concentration and the CSF/serum ratio were higher in untreated OMS than controls, related directly to OMS severity and inversely to OMS duration, and correlated with CSF B cell frequency and oligoclonal bands. CXCL12 showed the opposite pattern. Selective accumulation of CXCR5+ memory B cells in CSF was found. In ACTH-treated OMS, CXCL13, but not CXCL12, was lower. These data implicate the chemokine/chemoreceptor pair CXCL13/CXR5 in B cell recruitment to the CNS in OMS. CXCL13 and CXCL12 may serve as reciprocal biomarkers of disease activity, but CXCL13 also had utility as a treatment biomarker.
authors.affiliation: National Pediatric Myoclonus Center and Neuroimmunology Laboratory, Southern Illinois University School of Medicine, PO Box 19643, Springfield, IL 62794-9643, USA. mpranzatelli@siumed.edu
authors.forename: Michael R
authors.initials: MR
authors.lastname: Pranzatelli
authors.forename: Elizabeth D
authors.initials: ED
authors.lastname: Tate
authors.forename: Nathan R
authors.initials: NR
authors.lastname: McGee
authors.forename: Anna L
authors.initials: AL
authors.lastname: Travelstead
authors.forename: Richard M
authors.initials: RM
authors.lastname: Ransohoff
authors.forename: Jayne M
authors.initials: JM
authors.lastname: Ness
authors.forename: Jerry A
authors.initials: JA
authors.lastname: Colliver
mesh_terms: D000293:Adolescent; D000305:Adrenal Cortex Hormones / Q000627:therapeutic use; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology / Q000627:therapeutic use; D000704:Analysis of Variance; D015703:Antigens, CD / Q000378:metabolism; D001402:B-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D016022:Case-Control Studies; D002465:Cell Movement / Q000187:drug effects; D054377:Chemokine CXCL12 / Q000134:cerebrospinal fluid / Q000378:metabolism; D054382:Chemokine CXCL13 / Q000134:cerebrospinal fluid / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D003430:Cross-Sectional Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016756:Immunoglobulins, Intravenous / Q000627:therapeutic use; D007223:Infant; D008137:Longitudinal Studies; D008297:Male; D053578:Opsoclonus-Myoclonus Syndrome / Q000134:cerebrospinal fluid* / Q000188:drug therapy; D054380:Receptors, CXCR5 / Q000378:metabolism*
chemical_list: D000305:Adrenal Cortex Hormones; D015703:Antigens, CD; C515255:CXCL12 protein, human; C110663:CXCL13 protein, human; C095085:CXCR5 protein, human; D054377:Chemokine CXCL12; D054382:Chemokine CXCL13; D016756:Immunoglobulins, Intravenous; D054380:Receptors, CXCR5; D000324:Adrenocorticotropic Hormone