title: In vitro platelet quality in storage containers used for pediatric transfusions.
abstract: BACKGROUND
  The in vitro quality of small-volume platelet (PLT) aliquots for pediatric transfusions was assessed to determine the best practice approach.
  
  
  METHODS
  Small volumes (50 mL) of single apheresis PLT components (APCs), collected on either CaridianBCT Trima or Haemonetics MCS+ instruments, were aliquoted on Days 2, 3, 4, and 5 postcollection into Fenwal PL1240 or 4R2014 bags or 60-mL polypropylene syringes. Samples were tested for in vitro quality at their recommended expiry times (4 hr for 4R2014 bags and syringes or Day 5 for PL1240 bags). Assays included pH, CD62P expression, and metabolic measures.
  
  
  RESULTS
  CD62P expression increased throughout storage in all containers. Among the small-volume containers, pH, pCO(2) , lactate, and bicarbonate varied considerably. Regardless of the day of aliquoting, pCO(2) was significantly higher and pO(2) was significantly lower in gas-impermeable syringes than other containers. No bacterial growth was detected in any sample.
  
  
  CONCLUSIONS
  The quality of APCs aliquoted into small-volume containers meets regulatory requirements and is generally equivalent to that of full-volume APCs at expiry.
authors.affiliation: Canadian Blood Services Research and Development, Edmonton, Alberta, Canada.
authors.forename: Sandra
authors.initials: S
authors.lastname: Weiss
authors.forename: Kenneth
authors.initials: K
authors.lastname: Scammell
authors.forename: Elena
authors.initials: E
authors.lastname: Levin
authors.forename: Brankica
authors.initials: B
authors.lastname: Culibrk
authors.forename: Sima
authors.initials: S
authors.lastname: Zolfaghari
authors.forename: Maria I C
authors.initials: MI
authors.lastname: Gyöngyössy-Issa
authors.forename: Jason P
authors.initials: JP
authors.lastname: Acker
mesh_terms: D016824:Antigens, Human Platelet / Q000378:metabolism; D001639:Bicarbonates / Q000378:metabolism; D001771:Blood Banks / Q000592:standards*; D001792:Blood Platelets / Q000166:cytology* / Q000378:metabolism*; D001793:Blood Preservation / Q000295:instrumentation / Q000379:methods / Q000592:standards*; D002245:Carbon Dioxide / Q000378:metabolism; D002648:Child; D005434:Flow Cytometry; D005947:Glucose / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D019344:Lactic Acid / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D019007:P-Selectin / Q000378:metabolism; D010976:Platelet Count; D017713:Platelet Transfusion / Q000379:methods / Q000592:standards*; D017410:Practice Guidelines as Topic; D000094902:Blood Banking / Q000379:methods
chemical_list: D016824:Antigens, Human Platelet; D001639:Bicarbonates; D019007:P-Selectin; D002245:Carbon Dioxide; D019344:Lactic Acid; D005947:Glucose; D010100:Oxygen