title: Microfluidics-mediated isothermal detection of enzyme activity at the single molecule level.
abstract: Conventional analysis of enzymatic activity, often carried out on pools of cells, is blind to heterogeneity in the population. Here, we combine microfluidics with a previously developed isothermal rolling circle amplification-based assay to investigate multiple enzymatic activities in down to single cells. This microfluidics-meditated assay performs at very high sensitivity in picoliter incubators with small quantities of biological materials. Furthermore, we demonstrate the assay's capability of multiplexed detection of at least three enzyme activities at the single molecule level.
authors.affiliation: Department of Molecular Biology and Interdisciplinary Nanoscience Center, iNANO, Aarhus University, Denmark.
authors.forename: Sissel
authors.initials: S
authors.lastname: Juul
authors.forename: Yi-Ping
authors.initials: YP
authors.lastname: Ho
authors.forename: Magnus
authors.initials: M
authors.lastname: Stougaard
authors.forename: Jørn
authors.initials: J
authors.lastname: Koch
authors.forename: Felicie F
authors.initials: FF
authors.lastname: Andersen
authors.forename: Kam W
authors.initials: KW
authors.lastname: Leong
authors.forename: Birgitta R
authors.initials: BR
authors.lastname: Knudsen
mesh_terms: D002460:Cell Line; D004798:Enzymes / Q000378:metabolism*; D006801:Humans; D044085:Microfluidics
chemical_list: D004798:Enzymes