title: A novel anti-MUC1 antibody against the MUC1 cytoplasmic tail domain: use in sensitive identification of poorly differentiated cells in adenocarcinoma of the stomach.
abstract: BACKGROUND
  Isolated cancer cells of non-solid type poorly differentiated adenocarcinoma (por2) or signet-ring cell carcinoma (sig) are frequently seen in scirrhous gastric cancers with a very poor prognosis. These cells are often scattered in granulation tissue or desmoplastic fibrotic tissue and tend to be overlooked in routine pathological examination. We aimed to raise a novel antibody that can identify the isolated cancer cells easily.
  
  
  METHODS
  Because the MUC1 cytoplasmic tail domain (CTD) has many biological roles including tumor progression and cell adhesion disturbance and is expected to be expressed in isolated cancer cells, we raised a novel monoclonal antibody (MAb) MUC1-014E against an intracellular nonrepeating 19-amino-acid sequence (RYVPPSSTDRSPYEKVSAG: N-1217-1235-C) of the MUC1 CTD, using a synthetic peptide including the 7-amino-acid epitope (STDRSPY: N-1223-1229-C).
  
  
  RESULTS
  In the immunohistochemical staining of 107 gastrectomy specimens including 48 por2 and 31 sig lesions, the MAb MUC1-014E showed high rates of positive staining (≥5% of carcinoma cells stained) for por2 (100%) and sig (97%), and of the highest intensity staining (4+, ≥75% of carcinoma cells stained) for por2 (100%) and sig (90%). In the 89 biopsy specimens including 82 por2 and 38 sig lesions, the MAb MUC1-014E showed high rates of positive staining for por2 (100%) and sig (100%) and of 4+ staining for por2 (87%) and sig (84%). All the rates were significantly higher than those with cytokeratins (AE1/AE3 or CAM5.2).
  
  
  CONCLUSIONS
  The MAb MUC1-014E is very useful for accurate detection of isolated cancer cells in scirrhous gastric cancers.
authors.affiliation: Department of Human Pathology, Field of Oncology, Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1 Sakuragaoka, Kagoshima 890-8544, Japan. syoneza@m2.kufm.kagoshima-u.ac.jp
authors.forename: Suguru
authors.initials: S
authors.lastname: Yonezawa
authors.forename: Shinichi
authors.initials: S
authors.lastname: Kitajima
authors.forename: Michiyo
authors.initials: M
authors.lastname: Higashi
authors.forename: Masahiko
authors.initials: M
authors.lastname: Osako
authors.forename: Michiko
authors.initials: M
authors.lastname: Horinouchi
authors.forename: Seiya
authors.initials: S
authors.lastname: Yokoyama
authors.forename: Sho
authors.initials: S
authors.lastname: Kitamoto
authors.forename: Norishige
authors.initials: N
authors.lastname: Yamada
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Tamura
authors.forename: Takeshi
authors.initials: T
authors.lastname: Shimizu
authors.forename: Mineo
authors.initials: M
authors.lastname: Tabata
authors.forename: Masamichi
authors.initials: M
authors.lastname: Goto
mesh_terms: D000230:Adenocarcinoma / Q000276:immunology / Q000473:pathology* / Q000601:surgery; D002293:Adenocarcinoma, Scirrhous / Q000276:immunology / Q000473:pathology / Q000601:surgery; D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology; D018279:Carcinoma, Signet Ring Cell / Q000276:immunology / Q000473:pathology / Q000601:surgery; D002454:Cell Differentiation; D003593:Cytoplasm / Q000276:immunology / Q000473:pathology; D005743:Gastrectomy; D005753:Gastric Mucosa / Q000166:cytology / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D008969:Molecular Sequence Data; D018396:Mucin-1 / Q000276:immunology*; D017434:Protein Structure, Tertiary; D013274:Stomach Neoplasms / Q000276:immunology / Q000473:pathology* / Q000601:surgery
chemical_list: D000911:Antibodies, Monoclonal; C060192:MUC1 protein, human; D018396:Mucin-1