title: [Effect of beryllium on chemical elements of cell membrane and H2O2 production of Streptococcus oralis].
abstract: OBJECTIVE
  To evaluate the effect of beryllium (Be(2+)) on chemical elements of cell membrane and H2O2 production of Streptococcus oralis (S.oralis), thus to explore the microbiologic mechanisms of periodontal diseases which may occur after prosthodontic treatment.
  
  
  METHODS
  S.oralis was put into artificial saliva with different Be(2+) concentration (5mg/L, 10mg/L, 20mg/L and 40mg/L) and anaerobic cultured for 24 hours. The amount of chemical elements in the membrane of S.oralis were tested with X-ray energy dispersive spectroscopy, and the H(2)O(2) produced by S.oralis was detected with ABTS-HRP. The data were analyzed with one-way ANOVA using SPSS 11.0 software package.
  
  
  RESULTS
  The amount of calcium in the cell membrane of beryllium treated S. oralis decreased, while the amount of phosphorus increased. Natrium increased with the increase of Be(2+) in the culture, but changed contrarily when S. oralis was treated with 20mg/L Be(2+). H(2)O(2) produced by S. oralis reduced when the concentration of Be(2+) was at 40mg/L(P<0.05).
  
  
  CONCLUSIONS
  Beryllium affects the amount of chemical elements in cell membrane and H(2)O(2) production of S. oralis, which may result in a disturbance in the microecologic balance of subgingival microbes after proshodontic treatments and eventually contribute to periodontal diseases.
authors.affiliation: Department of Prosthodontics, School & Hospital of Stomatology, Whenzhou Medical College, Wenzhou 325027, Zhejiang Province, China. caraln@yahoo.cn
authors.forename: Jin-song
authors.initials: JS
authors.lastname: Liu
authors.forename: Zhen
authors.initials: Z
authors.lastname: Fan
authors.forename: Jian-feng
authors.initials: JF
authors.lastname: Ma
authors.forename: Ning
authors.initials: N
authors.lastname: Gao
mesh_terms: D001608:Beryllium; D002462:Cell Membrane; D006861:Hydrogen Peroxide; D010510:Periodontal Diseases; D019533:Streptococcus oralis
chemical_list: D006861:Hydrogen Peroxide; D001608:Beryllium