title: Study on the interaction between cyanobacteria FBP/SBPase and metal ions.
abstract: Fructose-1,6-/sedoheptulose-1,7-bisphosphatase (FBP/SBPase) is a potential important target enzyme for finding inhibitors to solve harmful algal bloom. In this paper, the interactions between FBP/SBPase and metal ions were studied by enzyme activity analysis, fluorescence and molecular modeling method. The enzyme activity analysis showed that FBP/SBPase can be activated by Mg2+ or Mn2+ but cannot be activated by Ca2+ or Zn2+. Spectroscopic analysis of emission quenching showed that quenching mechanism of FBP/SBPase with Mg2+ or Mn2+ was static quenching mechanism while that of Ca2+ or Zn2+ was dynamic quenching process. Hydrogen bonds and van der Waals interaction might be the predominant intermolecular forces in stabilizing FBP/SBPase-Mg2+ while hydrophobic forces were the predominant intermolecular forces in stabilizing FBP/SBPase-Mn2+. Microenvironment and conformation of FBP/SBPase were changed in binding reaction. The effect of metal ions and important amino acid residues on FBP/SBPase-metal ion complex was also discussed by molecular modeling study.
authors.affiliation: Key Laboratory of Pesticide & Chemical Biology (CCNU), Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, PR China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Sun
authors.forename: Xun
authors.initials: X
authors.lastname: Liao
authors.forename: Ding
authors.initials: D
authors.lastname: Li
authors.forename: Lingling
authors.initials: L
authors.lastname: Feng
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Jin
authors.forename: Fan
authors.initials: F
authors.lastname: Yi
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
authors.forename: Jian
authors.initials: J
authors.lastname: Wan
mesh_terms: D004789:Enzyme Activation; D006597:Fructose-Bisphosphatase / Q000737:chemistry / Q000378:metabolism*; D006860:Hydrogen Bonding; D007477:Ions; D008670:Metals / Q000378:metabolism*; D008958:Models, Molecular; D017433:Protein Structure, Secondary; D013050:Spectrometry, Fluorescence; D046939:Synechocystis / Q000737:chemistry / Q000201:enzymology* / Q000378:metabolism
chemical_list: D007477:Ions; D008670:Metals; D006597:Fructose-Bisphosphatase