title: Development of a bead-based multiplex immunoassay for simultaneous quantitative detection of IgG serum antibodies against measles, mumps, rubella, and varicella-zoster virus.
abstract: Enzyme-linked immunosorbent assay (ELISA) is normally used to quantify the amount of serum IgG antibodies against measles, mumps, rubella, and varicella-zoster virus (MMRV). However, this method is time- and material-consuming. Therefore, a multiplex immunoassay for the simultaneous quantitative detection of antibodies against MMRV was developed. In-house as well as commercially available antigens can be used, making the assay available for all laboratories. The multiplex assay is much more sensitive than the separate ELISAs and has a high specificity, and only 5 μl of serum is needed. Heterologous inhibition did not exceed 11.5%, while homologous inhibition varied between 91.3 and 97.9%. Good correlations with the in-house ELISAs for measles (R(2) = 0.98), mumps (R(2) = 0.97), and rubella (R(2) = 0.97) virus as well as with the ELISA kit for varicella-zoster virus (R(2) = 0.95) were obtained. In conclusion, the MMRV multiplex assay is a good alternative to the conventional ELISAs and suitable for use in serosurveillance and vaccine studies.
authors.affiliation: Laboratory for Infectious Diseases and Screening, National Institute of Public Health and the Environment, Bilthoven, The Netherlands. Gaby.Smits@rivm.nl
authors.forename: Gaby P
authors.initials: GP
authors.lastname: Smits
authors.forename: Pieter G
authors.initials: PG
authors.lastname: van Gageldonk
authors.forename: Leo M
authors.initials: LM
authors.lastname: Schouls
authors.forename: Fiona R M
authors.initials: FR
authors.lastname: van der Klis
authors.forename: Guy A M
authors.initials: GA
authors.lastname: Berbers
mesh_terms: D000328:Adult; D000914:Antibodies, Viral / Q000097:blood*; D019411:Clinical Laboratory Techniques / Q000379:methods*; D014645:Herpesvirus 3, Human / Q000276:immunology*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007074:Immunoglobulin G / Q000097:blood*; D008459:Measles virus / Q000276:immunology*; D008863:Microspheres; D009109:Mumps virus / Q000276:immunology*; D012412:Rubella virus / Q000276:immunology*; D012680:Sensitivity and Specificity; D044967:Serum / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G