title: Screening serum biomarker of knee osteoarthritis using a phage display technique.
abstract: OBJECTIVE
  To screen serum biomarker of knee osteoarthritis (OA) using a phage random peptide library.
  
  
  METHODS
  A phage random peptide library of random peptide 12-mers was screened with purified immunoglobulin G (IgG) from sera of knee OA patients. Patients with knee rheumatoid arthritis (RA), hip OA, non-erosive hand OA or erosive hand OA, and healthy volunteers were used as controls.
  
  
  RESULTS
  A phage clone with inserted peptide TGLESGHGPGDS (named KOA1) showed 90% positive reaction rate with the knee OA patients, significantly higher than that with the knee RA patients (27.8%), the non-erosive hand OA patients (34.3%), the erosive hand OA patients (31.3%) and the healthy controls (12.0%), but not the hip OA patients (82.5%).
  
  
  CONCLUSIONS
  The novel knee OA mimic peptide KOA1 identified with a random phage display peptide library and sera from knee OA patients could be a potential serum biomarker for knee OA.
authors.affiliation: Department of Orthopaedics, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, Hunan, PR China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Weng
authors.forename: Qiande
authors.initials: Q
authors.lastname: Liao
authors.forename: Kanghua
authors.initials: K
authors.lastname: Li
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.forename: Meng
authors.initials: M
authors.lastname: Mi
authors.forename: Da
authors.initials: D
authors.lastname: Zhong
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001172:Arthritis, Rheumatoid / Q000097:blood; D001323:Autoantibodies / Q000097:blood* / Q000378:metabolism; D015415:Biomarkers / Q000097:blood; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis / Q000302:isolation & purification; D008297:Male; D008875:Middle Aged; D009842:Oligopeptides / Q000235:genetics / Q000378:metabolism*; D010003:Osteoarthritis / Q000097:blood; D020370:Osteoarthritis, Knee / Q000097:blood* / Q000175:diagnosis* / Q000276:immunology; D019151:Peptide Library; D010455:Peptides / Q000235:genetics / Q000378:metabolism*; D012680:Sensitivity and Specificity
chemical_list: D001323:Autoantibodies; D015415:Biomarkers; D007074:Immunoglobulin G; D009842:Oligopeptides; D019151:Peptide Library; D010455:Peptides