title: Secreted clusterin in colon tumor cell models and its potential as diagnostic marker for colorectal cancer.
abstract: We studied the specific changes of the secreted protein clusterin and its cytoplasmic precursor regarding colorectal tumorigenesis, using in vitro differentiation of Caco-2 cells. In tumor-like stage, we observed an overexpression of both precursor and secreted clusterin, corroborated in the cell line SW-480. Noticeably, SW-620 cells (from a tumoral node, thus with metastatic capacity) did not show overexpression of either precursor or secreted clusterin, suggesting a downregulation related to local metastasis. We further investigated clusterin in serum, finding a significant increase in colorectal cancer patients, with 81% sensitivity, 79% specificity, and an area under the ROC curve of 0.85.
authors.affiliation: Departamento de Bioquímica, Genética e Inmunología, Universidad de Vigo, Vigo, Spain. anamrp@uvigo.es
authors.forename: A M
authors.initials: AM
authors.lastname: Rodríguez-Piñeiro
authors.forename: A
authors.initials: A
authors.lastname: García-Lorenzo
authors.forename: S
authors.initials: S
authors.lastname: Blanco-Prieto
authors.forename: P
authors.initials: P
authors.lastname: Alvarez-Chaver
authors.forename: F J
authors.initials: FJ
authors.lastname: Rodríguez-Berrocal
authors.forename: M Páez de la
authors.initials: MP
authors.lastname: Cadena
authors.forename: V S
authors.initials: VS
authors.lastname: Martínez-Zorzano
mesh_terms: D000230:Adenocarcinoma / Q000097:blood / Q000175:diagnosis / Q000473:pathology; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood / Q000378:metabolism*; D018938:Caco-2 Cells; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D051152:Clusterin / Q000097:blood* / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010641:Phenotype
chemical_list: D014408:Biomarkers, Tumor; D051152:Clusterin