title: Comparison of polysaccharides from two species of Ganoderma.
abstract: Ganoderma lucidum and Ganoderma sinense, known as Lingzhi in Chinese, are commonly used Chinese medicines with excellent beneficial health effects. Triterpenes and polysaccharides are usually considered as their main active components. However, the content of triterpenes differs significantly between the two species of Ganoderma. To date, a careful comparison of polysaccharides from the two species of Ganoderma has not been performed. In this study, polysaccharides from fruiting bodies of two species of Lingzhi collected from different regions of China were analyzed and compared based on HPSEC-ELSD and HPSEC-MALLS-RI analyses, as well as enzymatic digestion and HPTLC of acid hydrolysates. The results indicated that both the HPSEC-ELSD profiles and the molecular weights of the polysaccharides were similar. Enzymatic digestion showed that polysaccharides from all samples of Lingzhi could be hydrolyzed by pectinase and dextranase. HPTLC profiles of their TFA hydrolysates colored with different reagents and their monosaccharides composition were also similar.
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, University of Macau, Macao SAR 999078, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Xie
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.forename: De-Jun
authors.initials: DJ
authors.lastname: Hu
authors.forename: Jin-Ao
authors.initials: JA
authors.lastname: Duan
authors.forename: Yu-Ping
authors.initials: YP
authors.lastname: Tang
authors.forename: Shao-Ping
authors.initials: SP
authors.lastname: Li
mesh_terms: D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification*; D048690:Fruiting Bodies, Fungal / Q000737:chemistry*; D006026:Glycoside Hydrolases / Q000737:chemistry; D006868:Hydrolysis; D008027:Light; D008970:Molecular Weight; D009005:Monosaccharides / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification*; D020940:Reishi / Q000737:chemistry*; D012542:Scattering, Radiation; D014269:Trifluoroacetic Acid / Q000737:chemistry
chemical_list: D004365:Drugs, Chinese Herbal; D009005:Monosaccharides; D011134:Polysaccharides; D014269:Trifluoroacetic Acid; D006026:Glycoside Hydrolases