title: Depolymerization of insulin amyloid fibrils by albumin-modified magnetic fluid.
abstract: Pathogenesis of amyloid-related diseases is associated with the presence of protein amyloid deposits. Insulin amyloids have been reported in a patient with diabetes undergoing treatment by injection of insulin and causes problems in the production and storage of this drug and in pplication of insulin pumps. We have studied the interference of insulin amyloid fibrils with a series of 18 albumin magnetic fluids (MFBSAs) consisting of magnetite nanoparticles modified by different amounts of bovine serum albumin (w/w BSA/Fe₃O₄ from 0.005 up to 15). We have found that MFBSAs are able to destroy amyloid fibrils in vitro. The extent of fibril depolymerization was affected by nanoparticle physical-chemical properties (hydrodynamic diameter, zeta potential and isoelectric point) determined by the BSA amount present in MFBSAs. The most effective were MFBSAs with lower BSA/Fe₃O₄ ratios (from 0.005 to 0.1) characteristic of about 90% depolymerizing activity. For the most active magnetic fluids (ratios 0.01 and 0.02) the DC50 values were determined in the range of low concentrations, indicating their ability to interfere with insulin fibrils at stoichiometric concentrations. We assume that the present findings represent a starting point for the application of the active MFBSAs as therapeutic agents targeting insulin amyloidosis.
authors.affiliation: Department of Biophysics, Department of Magnetism, Institute of Experimental Physics, Slovak Academy of Sciences, Watsonova 47, 04001 Kosice, Slovakia.
authors.forename: Katarina
authors.initials: K
authors.lastname: Siposova
authors.forename: Martina
authors.initials: M
authors.lastname: Kubovcikova
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Bednarikova
authors.forename: Martina
authors.initials: M
authors.lastname: Koneracka
authors.forename: Vlasta
authors.initials: V
authors.lastname: Zavisova
authors.forename: Andrea
authors.initials: A
authors.lastname: Antosova
authors.forename: Peter
authors.initials: P
authors.lastname: Kopcansky
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Daxnerova
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Gazova
mesh_terms: D000682:Amyloid / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D002417:Cattle; D007328:Insulin / Q000737:chemistry* / Q000378:metabolism; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D000682:Amyloid; D007328:Insulin; D058185:Magnetite Nanoparticles; D012710:Serum Albumin, Bovine