title: Newcastle disease virus LaSota strain kills human pancreatic cancer cells in vitro with high selectivity.
abstract: BACKGROUND
  Pancreatic cancer is highly resistant to treatment. Previously, we showed that Newcastle disease virus (NDV) strain 73-T was highly cytotoxic to a range of tumor types in vitro and in vivo but the effects of NDV on pancreatic tumors are unknown. We determined the cytotoxicity of the lentogenic LaSota strain of NDV (NDV-LS) toward 7 different human pancreatic tumor cell lines and 4 normal human cell lines (keratinocytes, fibroblasts, pancreatic ductal cells, and vascular endothelial cells).
  
  
  METHODS
  Cytotoxicity assays used serially diluted NDV incubated for 96 hours post-infection. Cells were fixed, stained, and minimum cytotoxic plaque forming unit (PFU) doses were determined (n = 10-24/cell line).
  
  
  RESULTS
  Normal cells were killed only by high doses of NDV-LS. The cytotoxic doses for pancreatic ductal cells, fibroblasts, and vascular endothelial cells were 729, 626, and 1,217 plaque forming units, respectively. In contrast, most pancreatic cancer cells were killed by much lower doses. The doses for PL45, Panc 10.05, PANC-1, BxPC3, SU.86.86, Capan-1 and CFPAC-1 were 0.15, 0.41, 0.43, 0.55, 1.30, 17.1 and 153 plaque forming units, respectively.
  
  
  CONCLUSIONS
  Most pancreatic tumor cells were more than 700 times more sensitive to NDV-LS killing than normal cells. Such avirulent, lentogenic NDV strains may have therapeutic potential in the treatment of pancreatic cancers.
authors.affiliation: Division of Gastroenterology and Hepatology, Cook County-John H Stroger Hospital, Chicago, IL 60612, USA. rwalter@rush.edu
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Walter
authors.forename: Bashar M
authors.initials: BM
authors.lastname: Attar
authors.forename: Asad
authors.initials: A
authors.lastname: Rafiq
authors.forename: Sooraj
authors.initials: S
authors.lastname: Tejaswi
authors.forename: Megan
authors.initials: M
authors.lastname: Delimata
mesh_terms: D002460:Cell Line; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology / Q000821:virology*; D054884:Host-Pathogen Interactions; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000821:virology*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000821:virology*; D009522:Newcastle disease virus / Q000145:classification / Q000502:physiology*; D050504:Oncolytic Viruses / Q000502:physiology; D010183:Pancreatic Ducts / Q000166:cytology / Q000821:virology*; D010190:Pancreatic Neoplasms / Q000473:pathology / Q000821:virology