title: Biocontrol of Salmonella Typhimurium in RTE foods with the virulent bacteriophage FO1-E2.
abstract: Foodborne Salmonella infections are a major public health concern worldwide. Bacteriophages offer highly specific and effective biocontrol of such pathogens. We evaluated the broad host range, virulent phage FO1-E2 for reduction of Salmonella Typhimurium in different RTE foods. Samples were spiked with 1×10³ Salmonella cells and treated with 3×10⁸ pfu/g phage, and incubated for 6 days at 8 °C or 15 °C. At 8 °C, no viable cells remained following FO1-E2 application, corresponding to a more than 3 log₁₀ unit reduction. At 15 °C, application of phage lowered S. Typhimurium counts by 5 log units on turkey deli meat and in chocolate milk, and by 3 logs on hot dogs and in seafood. In egg yolk, an effect was observed only after 2 days, but not after 6 days. Phage particles retained their infectivity, although they were readily immobilized by the food matrix, resulting in loss of their ability to diffuse and infect target cells. At the end of the incubation period, phage-resistant Salmonella strains appeared which, however, were not able to compensate for the initial killing effect. Altogether, our data show that virulent phages such as FO1-E2 offer an effective biocontrol measure for Salmonella in foods.
authors.affiliation: Institute of Food, Nutrition, and Health, ETH Zurich, 8092 Zurich, Switzerland.
authors.forename: Susanne
authors.initials: S
authors.lastname: Guenther
authors.forename: Oliver
authors.initials: O
authors.lastname: Herzig
authors.forename: Lars
authors.initials: L
authors.lastname: Fieseler
authors.forename: Jochen
authors.initials: J
authors.lastname: Klumpp
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Loessner
mesh_terms: D000818:Animals; D004203:Disinfection / Q000379:methods; D004285:Dogs; D005506:Food Contamination / Q000517:prevention & control*; D005516:Food Microbiology; D008460:Meat; D011634:Public Health; D012475:Salmonella; D012478:Salmonella Food Poisoning / Q000517:prevention & control; D012483:Salmonella Phages / Q000472:pathogenicity*; D012486:Salmonella typhimurium; D014774:Virulence