title: Acceleration of astrocytic differentiation in neural stem cells surviving X-irradiation.
abstract: Neural stem cells (NSCs) are highly susceptible to DNA double-strand breaks; however, little is known about the effects of radiation in cells surviving radiation. Although the nestin-positive NSCs predominantly became glial fibrillary acidic protein (GFAP)-positive in differentiation-permissive medium, little or no cells were GFAP positive in proliferation-permissive medium. We found that more than half of the cells surviving X-rays became GFAP positive in proliferation-permissive medium. Moreover, localized irradiation stimulated differentiation of cells outside the irradiated area. These results indicate for the first time that ionizing radiation is able to stimulate astrocyte-specific differentiation of surviving NSCs, whose process is mediated both by the direct activation of nuclear factor-κB and by the indirect bystander effect induced by X-irradiation.
authors.affiliation: Department of Radiation Medical Sciences, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
authors.forename: Ayumi
authors.initials: A
authors.lastname: Ozeki
authors.forename: Keiji
authors.initials: K
authors.lastname: Suzuki
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Suzuki
authors.forename: Hiroki
authors.initials: H
authors.lastname: Ozawa
authors.forename: Shunichi
authors.initials: S
authors.lastname: Yamashita
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology* / Q000528:radiation effects*; D002454:Cell Differentiation / Q000528:radiation effects*; D049109:Cell Proliferation / Q000528:radiation effects; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007381:Intermediate Filament Proteins / Q000378:metabolism; D016328:NF-kappa B / Q000528:radiation effects; D009419:Nerve Tissue Proteins / Q000378:metabolism; D064231:Nestin; D058953:Neural Stem Cells / Q000528:radiation effects*; D051381:Rats; D014965:X-Rays / Q000009:adverse effects*
chemical_list: D005904:Glial Fibrillary Acidic Protein; D007381:Intermediate Filament Proteins; D016328:NF-kappa B; D009419:Nerve Tissue Proteins; C577486:Nes protein, rat; D064231:Nestin