title: Expression of PDGFR-β and Kit in canine anal sac apocrine gland adenocarcinoma using tissue immunohistochemistry.
abstract: Canine anal sac apocrine gland adenocarcinoma (ASAGAC) is an uncommon but highly invasive and metastatic malignancy. Toceranib phosphate (Palladia) is a receptor tyrosine kinase (RTK) inhibitor that targets several members of the split kinase RTK family. These membrane receptors are important for cell cycling, apoptosis and angiogenesis, all of which can contribute to carcinogenesis. The objective of this study was to evaluate archived, paraffin-embedded canine ASAGAC and normal canine anal sacs for immunohistochemical detection of Kit and platelet-derived growth factor receptor beta (PDGFR-β). Two of 77 neoplasms (2.6%) expressed Kit. Fifteen of the neoplasms (19.5%) were positive for PDGFR-β expression. None of the normal canine anal sac epithelium expressed Kit or PDGFR-β. Because of these results, further investigation should be considered to determine the role of RTKs in the clinical course and treatment of canine ASAGAC.
authors.affiliation: Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Tennessee, Knoxville, TN 37996-4544, USA. rward17@utk.edu
authors.forename: R J
authors.initials: RJ
authors.lastname: Brown
authors.forename: S J
authors.initials: SJ
authors.lastname: Newman
authors.forename: D C
authors.initials: DC
authors.lastname: Durtschi
authors.forename: A K
authors.initials: AK
authors.lastname: Leblanc
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry / Q000662:veterinary*; D000818:Animals; D001005:Anus Neoplasms / Q000737:chemistry / Q000662:veterinary*; D001050:Apocrine Glands / Q000737:chemistry* / Q000473:pathology; D004283:Dog Diseases / Q000201:enzymology*; D004285:Dogs; D007150:Immunohistochemistry / Q000662:veterinary; D016612:Paraffin Embedding / Q000662:veterinary; D019009:Proto-Oncogene Proteins c-kit / Q000032:analysis*; D020797:Receptor, Platelet-Derived Growth Factor beta / Q000032:analysis*; D012582:Schools, Veterinary; D013714:Tennessee
chemical_list: D019009:Proto-Oncogene Proteins c-kit; D020797:Receptor, Platelet-Derived Growth Factor beta