title: Cotrimoxazole enhances the in vitro susceptibility of Coccidioides posadasii to antifungals.
abstract: The aim of the present study was to evaluate the effect of cotrimoxazole on the in vitro susceptibility of Coccidioides posadasii strains to antifungals. A total of 18 strains of C. posadasii isolated in Brazil were evaluated in this study. The assays were performed in accordance with the Clinical and Laboratory Standards Institute guidelines and the combinations were tested using the checkerboard method. The minimum inhibitory concentrations were reduced by 11, 2.4, 4.3 and 3.5 times for amphotericin B, itraconazole, fluconazole and voriconazole, respectively. Moreover, it was seen that cotrimoxazole itself inhibited C. posadasii strains in vitro. The impairment of folic acid synthesis may be a potential antifungal target for C. posadasii.
authors.affiliation: Centro Especializado em Micologia Médica, Universidade Federal do Ceará, Fortaleza, CE, Brasil. rossanacordeiro@ufc.br
authors.forename: Rossana de Aguiar
authors.initials: Rde A
authors.lastname: Cordeiro
authors.forename: Delia Jessica
authors.initials: DJ
authors.lastname: Astete-Medrano
authors.forename: Francisca Jakelyne de Farias
authors.initials: FJ
authors.lastname: Marques
authors.forename: Heuziwanne Tavares Leite
authors.initials: HT
authors.lastname: Andrade
authors.forename: Lauro Vieira
authors.initials: LV
authors.lastname: Perdigão Neto
authors.forename: Juliane Lira
authors.initials: JL
authors.lastname: Tavares
authors.forename: Rita Amanda Chaves
authors.initials: RA
authors.lastname: de Lima
authors.forename: Kharla Kharolyni Nobre Rabelo
authors.initials: KK
authors.lastname: Patoilo
authors.forename: Andre Jalles
authors.initials: AJ
authors.lastname: Monteiro
authors.forename: Raimunda Sâmia Nogueira
authors.initials: RS
authors.lastname: Brilhante
authors.forename: Marcos Fábio Gadelha
authors.initials: MF
authors.lastname: Rocha
authors.forename: Zoilo Pires
authors.initials: ZP
authors.lastname: de Camargo
authors.forename: José Júlio Costa
authors.initials: JJ
authors.lastname: Sidrim
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D003045:Coccidioides / Q000145:classification / Q000187:drug effects*; D004357:Drug Synergism; D006801:Humans; D021261:Parasitic Sensitivity Tests / Q000379:methods; D013997:Time Factors; D014230:Triazoles / Q000494:pharmacology*; D015662:Trimethoprim, Sulfamethoxazole Drug Combination / Q000494:pharmacology*
chemical_list: D000935:Antifungal Agents; D014230:Triazoles; D015662:Trimethoprim, Sulfamethoxazole Drug Combination