title: Effects of indoleamine 2,3-dioxygenases in carbon tetrachloride-induced hepatitis model of rats.
abstract: Indoleamine 2,3-dioxygenase (IDO) converts tryptophan to l-kynurenine, and it is noted as a relevant molecule in promoting tolerance and suppressing adaptive immunity. In this study, to investigate the effects of IDO in carbon tetrachloride (CCl(4) )-induced hepatitis model, the levels of IDO enzymic activities in the mock group, the control group and the 1-methyl-D-tryptophan (1-MT)-treated group were confirmed by determination of l-kynurenine concentrations. Serum alanine aminotransferase levels in 1-MT-treated rats after CCl(4) injection significantly increased compared with those in mock and control groups. In CCl(4)-induced hepatitis models, tumour necrosis factor-α (TNF-α) is critical in the development of liver injury. The mRNA expression and secretion levels of TNF-α in the liver from 1-MT-treated rats were more enhanced compared with those in the mock and the control groups. Moreover, the levels of cytokine and chemokine from mock, control group and 1-MT-treated rats after treated with CCl(4) were analyzed by ELISA, and the level of interleukin-6 was found to increase in 1-MT-treated rats. It was concluded that the deficiency of IDO exacerbated liver injury in CCl(4)-induced hepatitis and its effect may be connected with TNF-α and interleukin-6.
authors.affiliation: Department of Nuclear Medicine, Shanghai 10th People's Hospital, Tongji University School of Medicine, Shanghai 200072, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.forename: Haidong
authors.initials: H
authors.lastname: Cai
authors.forename: Min
authors.initials: M
authors.lastname: Hou
authors.forename: Da
authors.initials: D
authors.lastname: Fu
authors.forename: Yushui
authors.initials: Y
authors.lastname: Ma
authors.forename: Qiong
authors.initials: Q
authors.lastname: Luo
authors.forename: Xueyu
authors.initials: X
authors.lastname: Yuan
authors.forename: Mingli
authors.initials: M
authors.lastname: Lv
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Zhang
authors.forename: Xianling
authors.initials: X
authors.lastname: Cong
authors.forename: Zhongwei
authors.initials: Z
authors.lastname: Lv
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D002251:Carbon Tetrachloride / Q000633:toxicity; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism*; D018925:Chemokines / Q000235:genetics / Q000378:metabolism; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase / Q000378:metabolism*; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D007737:Kynurenine / Q000097:blood; D008099:Liver / Q000187:drug effects; D008297:Male; D051381:Rats; D014364:Tryptophan / Q000031:analogs & derivatives / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
chemical_list: D018925:Chemokines; D016207:Cytokines; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D007737:Kynurenine; D014364:Tryptophan; D002251:Carbon Tetrachloride; D000410:Alanine Transaminase; C525396:1-methyltryptophan