title: Dechlorination of p,p'-DDTs coupled with sulfate reduction by novel sulfate-reducing bacterium Clostridium sp. BXM.
abstract: A novel non-dsrAB (without dissimilatory sulfite reductase genes) sulfate-reducing bacterium (SRB) Clostridium sp. BXM was isolated from a paddy soil. Incubation experiments were then performed to investigate the formation of reduced sulfur compounds (RSC) by Clostridium sp. BXM, and RSC-induced dechlorination of p,p'-DDT in culture medium and soil solution. The RSCs produced were 5.8 mM and 4.5 mM in 28 mM sulfate amended medium and soil solution respectively after 28-day cultivation. The p,p'-DDT dechlorination ratios were 74% and 45.8% for 5.8mM and 4.5 mM RSCs respectively at 6h. The metabolites of p,p'-DDT found in the two reaction systems were identified as p,p'-DDD and p,p'-DDE. The dechlorination pathways of p,p'-DDT to p,p'-DDD and p,p'-DDE were proposed, based on mass balance and dechlorination time-courses. The results indicated that RSC-induced natural dechlorination may play an important role in the fate of organochlorines.
authors.affiliation: College of Resources and Environment, Graduate University of Chinese Academy of Sciences, Beijing, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Bao
authors.forename: Zheng-Yi
authors.initials: ZY
authors.lastname: Hu
authors.forename: Xin-Jun
authors.initials: XJ
authors.lastname: Wang
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.forename: Yu-Xin
authors.initials: YX
authors.lastname: Ba
authors.forename: Jing
authors.initials: J
authors.lastname: Hua
authors.forename: Chun-You
authors.initials: CY
authors.lastname: Zhu
authors.forename: Min
authors.initials: M
authors.lastname: Zhong
authors.forename: Chun-Yan
authors.initials: CY
authors.lastname: Wu
mesh_terms: D001673:Biodegradation, Environmental; D003013:Clostridium / Q000302:isolation & purification / Q000378:metabolism*; D003634:DDT / Q000737:chemistry / Q000378:metabolism*; D054879:Halogenation; D006843:Hydrocarbons, Chlorinated / Q000737:chemistry / Q000378:metabolism; D015394:Molecular Structure; D010084:Oxidation-Reduction; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000737:chemistry / Q000378:metabolism*; D013431:Sulfates / Q000378:metabolism*
chemical_list: D006843:Hydrocarbons, Chlorinated; D012989:Soil Pollutants; D013431:Sulfates; D003634:DDT