title: [A preliminary study on the regulation mechanism of p38MAPK on MUC5AC in allergic rhinitis].
abstract: OBJECTIVE
  To detect the effect of p38 mitogen activated protein kinase (p38MAPK) and cyclooxygenase-2 (COX-2) on the expression of mucin5AC (MUC5AC) in human nasal mucosa induced by histamine in vitro, and to investigate the pathogenesis of mucus hypersecretion in allergic rhinitis (AR).
  
  
  METHODS
  Western blot was performed to detect the protein expressions of p38MAPK, COX-2 and MUC5AC in nasal mucosa induced by histamine or blocked by selective inhibitors of p38MAPK and COX-2 of different concentration gradient.
  
  
  RESULTS
  Weak expressions of p38MAPK. COX-2 and MUC5AC were detected in normal nasal mucosa in vitro. The protein expressions of p38MAPK. COX-2 and MUC5AC increased in nasal mucosa induced by histamine in a dose-dependent manner. The histamine induced protein expressions of COX-2 and MUC5AC were dose-dependently attenuated by selective inhibitor of COX-2, namely NS-398. No apparent influence of NS-398 on the expression of p38MAPK was observed. The histamine induced protein expressions of p38MAPK, C()X-2 and MUCbAC dose-dependently decreased after nasal mucosa was treated by selective inhibitor of p38MAPK, namely SB203580. And no significant change of MUC5AC protein expression induced by NS-398 or SB203580 was observed.
  
  
  CONCLUSIONS
  Our findings indicated that the histamine-induced increased expression of MUC5AC by activated p38MAPK/COX-2 may be a possible pathogenesis of mucus hypersecretion in AR.
authors.affiliation: Department of Otolaryngology--Head and Neck Surgery, Xuanwu Hospital, Capital Medical University, Beijing, 100053, China.
authors.forename: Zhenlin
authors.initials: Z
authors.lastname: Wang
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.forename: Yuan
authors.initials: Y
authors.lastname: Li
authors.forename: Qiuhang
authors.initials: Q
authors.lastname: Zhang
authors.forename: Qiuyi
authors.initials: Q
authors.lastname: Qu
authors.forename: Yan
authors.initials: Y
authors.lastname: Qi
mesh_terms: D000328:Adult; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D055271:Mucin 5AC / Q000378:metabolism*; D009297:Nasal Mucosa / Q000378:metabolism*; D012221:Rhinitis, Allergic, Perennial / Q000378:metabolism*; D015398:Signal Transduction; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
chemical_list: C524678:MUC5AC protein, human; D055271:Mucin 5AC; D048051:p38 Mitogen-Activated Protein Kinases