title: Antioxidant effect of Stryphnodendron rotundifolium Martius extracts from Cariri-Ceará State (Brazil): potential involvement in its therapeutic use.
abstract: Stryphnodendron rotundifolium is a phytotherapic used in the northeast of Brazil for the treatment of inflammatory processes which normally are associated with oxidative stress. Consequently, we have tested the antioxidant properties of hydroalcoholic (HAB) and aqueous extracts (AB) from the bark and aqueous extract (AL) from the leaves of Stryphnodendron rotundifolium to determine a possible association between antioxidant activity and the popular use of this plant. Free radical scavenger properties were assessed by the quenching of 1',1'-diphenil-2-picrylhydrazyl (DPPH) and the calculated IC(50) were: HAB = 5.4 ± 0.7, AB = 12.0 ± 2.6, and AL = 46.3 ± 12.3 µg/mL. Total phenolic contents were: HAB = 102.7 ± 2.8, AB = 114.4 ± 14.6, and AL = 93.8 ± 9.1 µg/mg plant). HPLC/DAD analyses indicated that gallic acid, catechin, rutin and caffeic acid were the major components of the crude extracts of S. rotundifolium. Plant extracts inhibited Fe(II)-induced lipid peroxidation in brain homogenates. Iron chelation was also investigated and only HBA exhibited a weak activity. Taken together, the results suggest that S. rotundifolium could be considered an effective agent in the prevention of diseases associated with oxidative stress.
authors.affiliation: Department of Biological Chemistry, Laboratory of Natural Products, Program of Post-Graduation in Molecular Bioprospection, Regional University of Cariri, Street Cel. Antônio Luiz 1161, Pimenta, 63105-000 Crato-CE, Brazil.
authors.forename: José Galberto Martins
authors.initials: JG
authors.lastname: da Costa
authors.forename: Gerlânia de Oliveira
authors.initials: Gde O
authors.lastname: Leite
authors.forename: Albys Ferrer
authors.initials: AF
authors.lastname: Dubois
authors.forename: Rodrigo Lopes
authors.initials: RL
authors.lastname: Seeger
authors.forename: Aline Augusti
authors.initials: AA
authors.lastname: Boligon
authors.forename: Margareth Linde
authors.initials: ML
authors.lastname: Athayde
authors.forename: Adriana Rolim
authors.initials: AR
authors.lastname: Campos
authors.forename: João Batista Teixeira
authors.initials: JB
authors.lastname: da Rocha
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000737:chemistry; D001921:Brain / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D001938:Brazil; D002851:Chromatography, High Pressure Liquid; D003855:Deoxyribose / Q000737:chemistry; D007887:Fabaceae / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification; D007502:Iron Chelating Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008519:Medicine, Traditional; D018384:Oxidative Stress; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification; D010851:Picrates / Q000737:chemistry; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D005419:Flavonoids; D007502:Iron Chelating Agents; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D017392:Thiobarbituric Acid Reactive Substances; D008315:Malondialdehyde; D003855:Deoxyribose; C004931:1,1-diphenyl-2-picrylhydrazyl