title: Comparative proteomic analysis of salt response proteins in seedling roots of two wheat varieties.
abstract: A comparative proteomic analysis was made of salt response in seedling roots of wheat cultivars Jing-411 (salt tolerant) and Chinese Spring (salt sensitive) subjected to a range of salt stress concentrations (0.5%, 1.5% and 2.5%) for 2 days. One hundred and ninety eight differentially expressed protein spots (DEPs) were located with at least two-fold differences in abundance on 2-DE maps, of which 144 were identified by MALDI-TOF-TOF MS. These proteins were involved primarily in carbon metabolism (31.9%), detoxification and defense (12.5%), chaperones (5.6%) and signal transduction (4.9%). Comparative analysis showed that 41 DEPs were salt responsive with significant expression changes in both varieties under salt stress, and 99 (52 in Jing-411 and 47 in Chinese Spring) were variety specific. Only 15 and 9 DEPs in Jing-411 and Chinese Spring, respectively, were up-regulated in abundance under all three salt concentrations. All dynamics of the DEPs were analyzed across all treatments. Some salt responsive DEPs, such as guanine nucleotide-binding protein subunit beta-like protein, RuBisCO large subunit-binding protein subunit alpha and pathogenesis related protein 10, were up-regulated significantly in Jing-411 under all salt concentrations, whereas they were down-regulated in salinity-stressed Chinese Spring.
authors.affiliation: Key Laboratory of Genetics and Biotechnology, College of Life Sciences, Capital Normal University, 100048 Beijing, China.
authors.forename: Guangfang
authors.initials: G
authors.lastname: Guo
authors.forename: Pei
authors.initials: P
authors.lastname: Ge
authors.forename: Chaoying
authors.initials: C
authors.lastname: Ma
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Li
authors.forename: Dongwen
authors.initials: D
authors.lastname: Lv
authors.forename: Shunli
authors.initials: S
authors.lastname: Wang
authors.forename: Wujun
authors.initials: W
authors.lastname: Ma
authors.forename: Yueming
authors.initials: Y
authors.lastname: Yan
mesh_terms: D018506:Gene Expression Regulation, Plant; D010940:Plant Proteins / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects* / Q000378:metabolism; D040901:Proteomics; D054712:Salinity; D036226:Seedlings / Q000187:drug effects / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology*; D014908:Triticum / Q000187:drug effects* / Q000235:genetics
chemical_list: D010940:Plant Proteins; D012965:Sodium Chloride