title: Progastrin-releasing peptide is a candidate marker for quality control in clinical sample processing and storage.
abstract: For clinical epidemiologic and proteomic studies, the control of preanalytic variation, including sample processing and storage, is important. We evaluated the stability of progastrin-releasing peptide (ProGRP) as a marker for the quality control of stored serum and plasma samples. The ProGRP from 23 healthy volunteers was measured serially for 8 hours at room temperature, and the results were validated with clinical samples from the biobank. A significant difference in ΔProGRP was also noted between good-quality (time delay <4 hours before storage) and poor-quality (time delay ≥4 hours before storage) specimens (mean ± SD, 0.17 ± 0.08 vs 0.36 ± 0.14; P < .001; Wilcoxon signed-rank test). With a ΔProGRP cutoff of 0.22, the sensitivity and specificity of detection of the poor-quality samples were 85.7% and 75.0%, respectively, in clinical validations. We demonstrated that ΔProGRP could be used as a marker for quality control in sample processing and storage in biobanks.
authors.affiliation: Dept. of Laboratory Medicine, Center for Diagnostic Oncology, National Cancer Center, 323 Ilsan-ro, Ilsandong-gu, Goyang-si, Gyeonggi-do, Korea.
authors.forename: Shine-Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Ji-Su
authors.initials: JS
authors.lastname: Kim
authors.forename: Sang-Hyun
authors.initials: SH
authors.lastname: Hwang
authors.forename: Hye-Kyung
authors.initials: HK
authors.lastname: Park
authors.forename: Jin-Hyeon
authors.initials: JH
authors.lastname: Lee
authors.forename: Do-Hoon
authors.initials: DH
authors.lastname: Lee
authors.forename: Sang-Soo
authors.initials: SS
authors.lastname: Hah
authors.forename: Do Youn
authors.initials: DY
authors.lastname: Park
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010446:Peptide Fragments / Q000378:metabolism*; D011785:Quality Assurance, Health Care / Q000379:methods*; D012372:ROC Curve; D011994:Recombinant Proteins / Q000378:metabolism; D015203:Reproducibility of Results; D013048:Specimen Handling / Q000379:methods* / Q000592:standards; D013997:Time Factors
chemical_list: D015415:Biomarkers; D010446:Peptide Fragments; D011994:Recombinant Proteins; C087079:pro-gastrin-releasing peptide (31-98)