title: Determination of ganciclovir in human plasma by ultra performance liquid chromatography-UV detection.
abstract: OBJECTIVE
  Implement a sensitive UHPLC method for the assay of ganciclovir in human plasma.
  
  
  METHODS
  We developed and validated a chromatographic method coupled to ultraviolet detection for quantification of ganciclovir, with a short run time using a small volume of human plasma. Comparison of system performance was made with respect to analysis time, efficiency and sensitivity.
  
  
  RESULTS
  Correlation coefficients (r) of the calibration curves ranged from 0.999744 to 0.999784. Within-day and between-day imprecision and inaccuracy, specificity and recovery were also evaluated for validation. The method was precise and accurate and the retention time was 0.7 min. The calibration curves were linear between 0.5 and 30 μg/mL. There was a good correlation between HPLC and UHPLC techniques.
  
  
  CONCLUSIONS
  We developed a method that is currently applied in a clinical study assessing GCV plasma concentration variability after ganciclovir and valganciclovir administration.
authors.affiliation: Pharmacy Department, IDIBELL, Hospital Universitari de Bellvitge, L'hospitalet de Llobregat, Barcelona, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Padullés
authors.forename: H
authors.initials: H
authors.lastname: Colom
authors.forename: Y
authors.initials: Y
authors.lastname: Armendariz
authors.forename: G
authors.initials: G
authors.lastname: Cerezo
authors.forename: A
authors.initials: A
authors.lastname: Caldes
authors.forename: L
authors.initials: L
authors.lastname: Pou
authors.forename: J
authors.initials: J
authors.lastname: Torras
authors.forename: J M
authors.initials: JM
authors.lastname: Grinyó
authors.forename: N
authors.initials: N
authors.lastname: Lloberas
mesh_terms: D000998:Antiviral Agents / Q000097:blood*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D015774:Ganciclovir / Q000097:blood*; D006801:Humans; D057230:Limit of Detection; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000998:Antiviral Agents; D015774:Ganciclovir