title: Nonmuscle myosin IIA facilitates vesicle trafficking for MG53-mediated cell membrane repair.
abstract: Repair of injury to the plasma membrane is an essential mechanism for maintenance of cellular homeostasis and integrity that involves coordinated movement of intracellular vesicles to membrane injury sites to facilitate patch formation. We have previously identified MG53 as an essential component of the cell membrane repair machinery. In order for MG53 and intracellular vesicles to translocate to membrane injury sites, motor proteins must be involved. Here, we show that nonmuscle myosin type IIA (NM-IIA) interacts with MG53 to regulate vesicle trafficking during cell membrane repair. In cells that are deficient for NM-IIA expression, MG53 cannot translocate to acute injury sites, whereas rescue of NM-IIA expression in these cells can restore MG53-mediated membrane repair. Compromised cell membrane repair is observed in cells with RNAi-mediated knockdown of NM-IIA expression, or following pharmacological alteration of NM-IIA motor function. Together, our data reveal NM-IIA as a key cytoskeleton motor protein that facilitates vesicle trafficking during MG53-mediated cell membrane repair.
authors.affiliation: Department of Physiology and Biophysics, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA.
authors.forename: Peihui
authors.initials: P
authors.lastname: Lin
authors.forename: Hua
authors.initials: H
authors.lastname: Zhu
authors.forename: Chuanxi
authors.initials: C
authors.lastname: Cai
authors.forename: Xianhua
authors.initials: X
authors.lastname: Wang
authors.forename: Chunmei
authors.initials: C
authors.lastname: Cao
authors.forename: Ruiping
authors.initials: R
authors.lastname: Xiao
authors.forename: Zui
authors.initials: Z
authors.lastname: Pan
authors.forename: Noah
authors.initials: N
authors.lastname: Weisleder
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takeshima
authors.forename: Jianjie
authors.initials: J
authors.lastname: Ma
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D002352:Carrier Proteins / Q000502:physiology*; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology; D002522:Chlorocebus aethiops; D017931:DNA Primers; D006801:Humans; D008969:Molecular Sequence Data; D024507:Nonmuscle Myosin Type IIA / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D021381:Protein Transport; D034622:RNA Interference; D000070584:Tripartite Motif Proteins
chemical_list: D002352:Carrier Proteins; D017931:DNA Primers; C558399:TRIM72 protein, human; D000070584:Tripartite Motif Proteins; D024507:Nonmuscle Myosin Type IIA