title: Comparison of CD34, CD31, and factor VIII-related antigen immunohistochemical expression in feline vascular neoplasms and CD34 expression in feline nonvascular neoplasms.
abstract: The diagnosis of vascular neoplasms is often facilitated by the use of immunohistochemical markers such as factor VIII-related antigen, CD31, and CD34. However, the relative sensitivity and specificity of these markers have not been compared in cat vascular neoplasms. In this study, these 3 immunohistochemical markers were evaluated in 61 endothelial neoplasms (50 hemangiosarcomas and 11 hemangiomas) in 59 cats. All neoplasms were labeled by all 3 markers. CD34 had the highest average immunolabeling intensity in neoplastic endothelial cells. CD31 had the lowest average background labeling, followed by CD34 and factor VIII-related antigen, respectively. CD34 expression was also examined in 130 nonvascular neoplasms of cats; 14 of 62 epithelial neoplasms, 39 of 43 mesenchymal neoplasms, 8 of 23 leukocytic neoplasms, and 2 of 2 melanomas were positive. Given the broad expression of CD34 in mesenchymal neoplasms, this marker has limited diagnostic relevance for vascular neoplasms of cats.
authors.affiliation: Animal Disease Diagnostic Laboratory and Department of Comparative Pathobiology, Purdue University, 406 South University, West Lafayette, IN 47907, USA.
authors.forename: R N
authors.initials: RN
authors.lastname: Jennings
authors.forename: M A
authors.initials: MA
authors.lastname: Miller
authors.forename: J A
authors.initials: JA
authors.lastname: Ramos-Vara
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002371:Cat Diseases / Q000378:metabolism*; D002415:Cats; D005169:Factor VIII / Q000378:metabolism; D006391:Hemangioma / Q000378:metabolism / Q000662:veterinary*; D006394:Hemangiosarcoma / Q000378:metabolism / Q000662:veterinary*; D007150:Immunohistochemistry / Q000662:veterinary; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D012680:Sensitivity and Specificity; D019043:Vascular Neoplasms / Q000378:metabolism / Q000662:veterinary*
chemical_list: D018952:Antigens, CD34; D014408:Biomarkers, Tumor; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D005169:Factor VIII