title: Human polyomaviruses JC and BK in the urine of Brazilian children and adolescents vertically infected by HIV.
abstract: The aim of this study was to characterize the urinary excretion of the BK (BKV) and JC (JCV) human polyomaviruses in a cohort of human immunodeficiency virus (HIV)-infected children and adolescents. One hundred and fifty-six patients were enrolled: Group I included 116 HIV-infected children and adolescents [median age = 11.4 years (y); range 1-22 y]; Group II included 40 non-HIV-infected healthy controls (median age = 11.37 y; range 7-16 y). Single urine samples from both groups were screened for the presence of JCV and BKV DNA by polymerase chain reaction at enrolment. The overall rate of JCV and BKV urinary excretion was found to be 24.4% and 40.4%, respectively (n = 156). Group I had urinary excretion of JCV and BKV in 27.6% and 54.3% of subjects, respectively. In contrast, Group II showed positive results for JCV in 17.5% of subjects and for BKV in 12.5% of subjects (p Pearson JCV = 0.20; p Pearson BKV < 0.0001). In Group I, there was no association between JCV/BKV shedding and age, gender or CD4 values. Patients with an HIV viral load < 50 copies/mL had a lower excretion of BKV (p < 0.001) and a trend of lower JCV excretion (p = 0.07). One patient in Group I (1/116, 0.9%) showed clinical and radiological features consistent with progressive multifocal leukoencephalopathy, suggesting that children with HIV/polyomavirus coinfection should be kept under surveillance.
authors.affiliation: Laboratório de Virologia, Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, SP, Brasil, 05403-000. dm.machado@uol.com.br
authors.forename: Daisy Maria
authors.initials: DM
authors.lastname: Machado
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Fink
authors.forename: Cláudio Sérgio
authors.initials: CS
authors.lastname: Pannuti
authors.forename: Regina Célia de Menezes
authors.initials: RC
authors.lastname: Succi
authors.forename: Alessandra Aparecida
authors.initials: AA
authors.lastname: Machado
authors.forename: Fabiana Bononi do
authors.initials: FB
authors.lastname: Carmo
authors.forename: Aída de Fátima Barbosa
authors.initials: Ade F
authors.lastname: Gouvêa
authors.forename: Paulo Roberto
authors.initials: PR
authors.lastname: Urbano
authors.forename: Suenia Vasconcelos
authors.initials: SV
authors.lastname: Beltrão
authors.forename: Isabel Cristina Lopes dos
authors.initials: IC
authors.lastname: Santos
authors.forename: Clarisse Martins
authors.initials: CM
authors.lastname: Machado
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000652:urine / Q000821:virology*; D000293:Adolescent; D001739:BK Virus / Q000235:genetics / Q000302:isolation & purification*; D018791:CD4 Lymphocyte Count; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D015331:Cohort Studies; D004279:DNA, Viral / Q000652:urine; D005260:Female; D006801:Humans; D007223:Infant; D007577:JC Virus / Q000235:genetics / Q000302:isolation & purification*; D008297:Male; D016133:Polymerase Chain Reaction; D027601:Polyomavirus Infections / Q000652:urine*; D014412:Tumor Virus Infections / Q000652:urine*; D019562:Viral Load; D055815:Young Adult
chemical_list: D004279:DNA, Viral