title: Effect of sweet wormwood Artemisia annua crude leaf extracts on some biological and physiological characteristics of the lesser mulberry pyralid, Glyphodes pyloalis.
abstract: The lesser mulberry pyralid, Glyphodes pyloalis Walker (Lepidoptera: Pyralidae) is a monophagous and dangerous pest of mulberry that has been recently observed in Guilan province, northern Iran. In this study, the crude methanol extract of sweet wormwood Artemisia annua L. (Asterales: Asteracaea) was investigated on toxicity, biological and physiological characteristics of this pest under controlled conditions (24 ± 1 °C, 75 ± 5% RH, and 16:8 L:D photoperiod). The effect of acute toxicity and sublethal doses on physiological characteristics was performed by topical application. The LC₅₀ and LC₂₀ values on fourth instar larvae were calculated as 0.33 and 0.22 gram leaf equivalent/ mL, respectively. The larval duration of fifth instar larvae in LC₅₀ treatment was prolonged (5.8 ± 0.52 days) compared with the control group (4.26 ± 0.29 days). However larval duration was reduced in the LC₂₀ treatment. The female adult longevity in the LC₅₀ dose was the least (4.53 ± 0.3 days), while longevity among controls was the highest (9.2 ± 0.29 days). The mean fecundity of adults after larval treatment with LC₅₀ was recorded as 105.6 ± 16.84 eggs/female, while the control was 392.74 ± 22.52 eggs/female. The percent hatchability was reduced in all treatments compared with the control. The effect of extract in 0.107, 0.053, 0.026 and 0.013 gle/mL on biochemical characteristics of this pest was also studied. The activity of α-amylase and protease 48 hours post-treatment was significantly reduced compared with the control. Similarly lipase, esterase, and glutathione S-transferase activity were significantly affected by A. annua extract.
authors.affiliation: Department of Plant Protection, College of Agriculture, University of Guilan, Rasht, Iran.
authors.forename: Roya
authors.initials: R
authors.lastname: Khosravi
authors.forename: Jalal Jalali
authors.initials: JJ
authors.lastname: Sendi
authors.forename: Mohammad
authors.initials: M
authors.lastname: Ghadamyari
authors.forename: Elham
authors.initials: E
authors.lastname: Yezdani
mesh_terms: D000818:Animals; D031172:Artemisia annua; D005260:Female; D007814:Larva / Q000187:drug effects; D007928:Lethal Dose 50; D008136:Longevity / Q000187:drug effects; D008297:Male; D031625:Morus / Q000469:parasitology; D009036:Moths / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development; D052287:Oviparity / Q000187:drug effects; D010936:Plant Extracts / Q000633:toxicity*; D011679:Pupa / Q000187:drug effects
chemical_list: D010936:Plant Extracts