title: Cytotoxicity, oxidative stress and expression of adhesion molecules in human umbilical vein endothelial cells exposed to dust from paints with or without nanoparticles.
abstract: Nanoparticles in primary form and nanoproducts might elicit different toxicological responses. We compared paint-related nanoparticles with respect to effects on endothelial oxidative stress, cytotoxicity and cell adhesion molecule expression. Primary human umbilical vein endothelial cells were exposed to primary nanoparticles (fine, photocatalytic or nanosized TiO(2), aluminium silicate, carbon black, nano-silicasol or axilate) and dust from sanding reference- or nanoparticle-containing paints. Most of the samples increased cell surface expressions of vascular cell adhesion molecule-1 (VCAM-1) and intracellular adhesion molecule-1 (ICAM-1), but paint sanding dust samples generally generated less response than primary particles of TiO(2) and carbon black. We found no relationship between the expression of adhesion molecules, cytotoxicity and production of reactive oxygen species. In conclusion, sanding dust from nanoparticle-containing paint did not generate more oxidative stress or expression of cell adhesion molecules than sanding dust from paint without nanoparticles, whereas the primary particles had the largest effect on mass basis.
authors.affiliation: Department of Public Health, University of Copenhagen , Øster Farimagsgade 5, Copenhagen K , Denmark.
authors.forename: Lone
authors.initials: L
authors.lastname: Mikkelsen
authors.forename: Keld A
authors.initials: KA
authors.lastname: Jensen
authors.forename: Ismo K
authors.initials: IK
authors.lastname: Koponen
authors.forename: Anne T
authors.initials: AT
authors.lastname: Saber
authors.forename: Håkan
authors.initials: H
authors.lastname: Wallin
authors.forename: Steffen
authors.initials: S
authors.lastname: Loft
authors.forename: Ulla
authors.initials: U
authors.lastname: Vogel
authors.forename: Peter
authors.initials: P
authors.lastname: Møller
mesh_terms: D000538:Aluminum Silicates / Q000633:toxicity; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004391:Dust; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D053768:Metal Nanoparticles / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D010150:Paint / Q000633:toxicity*; D010316:Particle Size; D017382:Reactive Oxygen Species / Q000378:metabolism; D053260:Soot / Q000633:toxicity; D014025:Titanium / Q000633:toxicity; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism
chemical_list: D000538:Aluminum Silicates; D015815:Cell Adhesion Molecules; D004391:Dust; C573420:ICAM1 protein, human; D017382:Reactive Oxygen Species; D053260:Soot; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; C009495:titanium dioxide; D014025:Titanium