title: Flagellin from recombinant attenuated Salmonella enterica serovar Typhimurium reveals a fundamental role in chicken innate immunity.
abstract: Recombinant attenuated Salmonella vaccines have been extensively studied, with a focus on eliciting specific immune responses against foreign antigens. However, very little is known about the innate immune responses, particularly the role of flagellin, in the induction of innate immunity triggered by recombinant attenuated Salmonella in chickens. In the present report, we describe two Salmonella enterica serovar Typhimurium vaccine strains, wild-type (WT) or flagellin-deficient (flhD) Salmonella, both expressing the fusion protein (F) gene of Newcastle disease virus. We examined the bacterial load and spatiotemporal kinetics of expression of inflammatory cytokine, chemokine, and Toll-like receptor 5 (TLR5) genes in the cecum, spleen, liver, and heterophils following oral immunization of chickens with the two Salmonella strains. The flhD mutant exhibited an enhanced ability to establish systemic infection compared to the WT. In contrast, the WT strain induced higher levels of interleukin-1β (IL-1β), CXCLi2, and TLR5 mRNAs in cecum, the spleen, and the heterophils than the flhD mutant at different times postinfection. Collectively, the present data reveal a fundamental role of flagellin in the innate immune responses induced by recombinant attenuated Salmonella vaccines in chickens that should be considered for the rational design of novel vaccines for poultry.
authors.affiliation: Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, People's Republic of China.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Pan
authors.forename: Qiuxia
authors.initials: Q
authors.lastname: Cong
authors.forename: Shizhong
authors.initials: S
authors.lastname: Geng
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fang
authors.forename: Xilong
authors.initials: X
authors.lastname: Kang
authors.forename: Meng
authors.initials: M
authors.lastname: You
authors.forename: Xinan
authors.initials: X
authors.lastname: Jiao
mesh_terms: D000818:Animals; D002432:Cecum / Q000276:immunology; D002645:Chickens; D005408:Flagellin / Q000235:genetics / Q000276:immunology*; D017353:Gene Deletion; D020869:Gene Expression Profiling; D007113:Immunity, Innate; D007378:Interleukins / Q000096:biosynthesis; D008099:Liver / Q000276:immunology; D009522:Newcastle disease virus / Q000235:genetics / Q000276:immunology; D011201:Poultry Diseases / Q000276:immunology* / Q000517:prevention & control; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D012481:Salmonella Infections, Animal / Q000276:immunology* / Q000517:prevention & control; D022562:Salmonella Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D012486:Salmonella typhimurium / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D013154:Spleen / Q000276:immunology; D051198:Toll-Like Receptor 5 / Q000096:biosynthesis; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014760:Viral Fusion Proteins / Q000235:genetics / Q000276:immunology; D037521:Virulence Factors / Q000172:deficiency / Q000276:immunology
chemical_list: D007378:Interleukins; D011993:Recombinant Fusion Proteins; D022562:Salmonella Vaccines; D051198:Toll-Like Receptor 5; D014613:Vaccines, Attenuated; D014760:Viral Fusion Proteins; D037521:Virulence Factors; D005408:Flagellin