title: The MCP-1 (CCL2) -2518 GG genotype is associated with protection against pulmonary tuberculosis in Moroccan patients.
abstract: BACKGROUND
  Both monocyte chemoattractant protein-1 (MCP-1), also designated officially as chemokine (C-C motif) ligand 2 (CCL2), and interleukin-12 p40 (IL-12 p40) molecules, encoded by polymorphic genes, are central components of the immune response to infection by Mycobacterium tuberculosis (Mtb). Their genetic diversity has previously been associated with the outcome of tuberculosis (TB) infection. We investigated whether the MCP-1 -2518 A/G and the IL-12B (p40) +1188 A/C polymorphisms influence susceptibility to or resistance against pulmonary tuberculosis (PTB) in a Moroccan population group.
  
  
  METHODS
  Genomic DNA from 337 patients along with 204 healthy controls were genotyped for the above-mentioned genetic variations using polymerase chain reaction-based restriction fragment length polymorphism assay.
  
  
  RESULTS
  We found a higher prevalence of homozygous MCP-1 -2518 G allele in healthy individuals than in patients (pc = 0.04; odds ratio  = 0.35; 95% confidence interval  = 0.13 - 0.86), suggesting a potential protective effect, whereas analysis of IL-12B +1188 variation failed to reveal any such association.
  
  
  CONCLUSIONS
  Our results are in agreement with recent findings in Ghanaian patients, complying with the known genetic admixture of the Moroccan population.
authors.affiliation: Immunology and Virology Department, National Institute of Hygiene, Agdal, Rabat, Morocco. arjinaima@yahoo.fr
authors.forename: Naima
authors.initials: N
authors.lastname: Arji
authors.forename: Marc
authors.initials: M
authors.lastname: Busson
authors.forename: Ghali
authors.initials: G
authors.lastname: Iraqi
authors.forename: Jamal Eddine
authors.initials: JE
authors.lastname: Bourkadi
authors.forename: Abdelaziz
authors.initials: A
authors.lastname: Benjouad
authors.forename: Wahid
authors.initials: W
authors.lastname: Boukouaci
authors.forename: Ouafae
authors.initials: O
authors.lastname: Lahlou
authors.forename: Jouda
authors.initials: J
authors.lastname: Ben Amor
authors.forename: Rajagopal
authors.initials: R
authors.lastname: Krishnamoorthy
authors.forename: Dominique
authors.initials: D
authors.lastname: Charron
authors.forename: Rajae
authors.initials: R
authors.lastname: El Aouad
authors.forename: Ryad
authors.initials: R
authors.lastname: Tamouza
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D044383:Black People / Q000235:genetics*; D016022:Case-Control Studies; D018932:Chemokine CCL2 / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D053762:Interleukin-12 Subunit p40 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009018:Morocco; D009169:Mycobacterium tuberculosis / Q000472:pathogenicity; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D014397:Tuberculosis, Pulmonary / Q000453:epidemiology / Q000208:ethnology / Q000235:genetics* / Q000382:microbiology; D055815:Young Adult
chemical_list: C111835:CCL2 protein, human; D018932:Chemokine CCL2; C500879:IL12B protein, human; D053762:Interleukin-12 Subunit p40