title: Hypersensitivity testing for Aspergillus fumigatus IgE is significantly more sensitive than testing for Aspergillus niger IgE.
abstract: We sought to determine if sufficient redundancy exists between specific IgE testing for Aspergillus fumigatus and Aspergillus niger to eliminate one of the assays in determining Aspergillus hypersensitivity. We reviewed regional laboratory results comparing A fumigatus-specific IgE with A niger-specific IgE using the Pharmacia UniCAP system (Pharmacia, Kalamazoo, MI). By using the Fisher exact test as an index of concordance among paired results, we showed a significant difference between 109 paired samples for the presence of specific IgE to A fumigatus and A niger (P < .0001). Of these specimens, 94 were negative for IgE to both species, 10 were positive for A fumigatus and negative for A niger; no specimen was positive for A niger and negative for A fumigatus. We conclude that A fumigatus-specific IgE is sufficient to detect Aspergillus hypersensitivity. The assay for A niger-specific IgE is redundant, less sensitive, and unnecessary if the assay for specific IgE for A fumigatus is performed.
authors.affiliation: Special Diagnostic Immunology Laboratory, Dept. of Medicine, Hackensack University Medical Center, 30 Prospect Avenue, Hackensack, NJ 07601, USA.
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Selvaggi
authors.forename: Jeremy P
authors.initials: JP
authors.lastname: Walco
authors.forename: Sujal
authors.initials: S
authors.lastname: Parikh
authors.forename: Gary A
authors.initials: GA
authors.lastname: Walco
mesh_terms: D000485:Allergens / Q000276:immunology; D000946:Antigens, Fungal / Q000276:immunology; D001228:Aspergillosis / Q000175:diagnosis / Q000276:immunology / Q000382:microbiology; D001232:Aspergillus fumigatus / Q000276:immunology* / Q000302:isolation & purification; D001234:Aspergillus niger / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis / Q000276:immunology*; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D012130:Respiratory Hypersensitivity / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology
chemical_list: D000485:Allergens; D000946:Antigens, Fungal; D007073:Immunoglobulin E