title: Studies on the effect of an Enterococcus faecium probiotic on T cell populations in peripheral blood and intestinal epithelium and on the susceptibility to Salmonella during a challenge infection with Salmonella Typhimurium in piglets.
abstract: Although Enterococcus faecium is used as a probiotic feed supplement in animal production, feeding of the bacterium to piglets resulted in a more severe infection with Salmonella Typhimurium DT104 during a challenge experiment. To enlighten the mode of action by which E. faecium affected the piglets' health, we investigated the influence of the probiotic bacterium on the development of intestinal and circulating immune cells during a challenge experiment with S. Typhimurium DT104. To minimise varying impacts of the maternal immunity on the course of infection, only piglets were implemented that descended from Salmonella-free sows. In addition, the potency of purified blood and intraepithelial immune cells to control the growth of Salmonella was tested in vitro. In animals treated with E. faecium, a reduction of intraepithelial CD8alphabeta T cells, reduced circulating CD8alphabeta T cells and a less efficient control of intracellular Salmonella growth, mediated by peripheral blood mononuclear cells, were observed.
authors.affiliation: Institute of Immunology and Molecular Biology, Department of Veterinary Medicine, Freie Universität Berlin, Berlin, Germany.
authors.forename: Hassan
authors.initials: H
authors.lastname: Mafamane
authors.forename: István
authors.initials: I
authors.lastname: Szabó
authors.forename: Michael F G
authors.initials: MF
authors.lastname: Schmidt
authors.forename: Matthias
authors.initials: M
authors.lastname: Filter
authors.forename: Nicole
authors.initials: N
authors.lastname: Walk
authors.forename: Karsten
authors.initials: K
authors.lastname: Tedin
authors.forename: Lydia
authors.initials: L
authors.lastname: Scharek-Tedin
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D002460:Cell Line; D004032:Diet / Q000662:veterinary; D016984:Enterococcus faecium / Q000502:physiology*; D004847:Epithelial Cells / Q000276:immunology / Q000382:microbiology; D005260:Female; D007413:Intestinal Mucosa / Q000166:cytology*; D017493:Leukocyte Common Antigens / Q000378:metabolism; D011247:Pregnancy; D019936:Probiotics; D012481:Salmonella Infections, Animal / Q000382:microbiology / Q000517:prevention & control*; D012486:Salmonella typhimurium / Q000502:physiology*; D013154:Spleen / Q000166:cytology; D013552:Swine; D013553:Swine Diseases / Q000097:blood / Q000382:microbiology / Q000517:prevention & control*; D013601:T-Lymphocytes / Q000502:physiology*
chemical_list: D017493:Leukocyte Common Antigens