title: Proteome analysis of cerebrospinal fluid in healthy beagles and canine encephalitis.
abstract: We performed proteomics analysis of the cerebrospinal fluid (CSF) of healthy dogs and dogs with meningoencephalitis of unknown etiology (MUE). By comparing two-dimensional electrophoreses (2DE), an upregulated spot was found in MUE dogs. This protein was identified as a neuron-specific enolase (NSE) by analysis with MALDI-TOF mass spectrometry. In comparing dot blots using an antibody against NSE, the NSE levels in the CSF of MUE dogs was significantly higher than that of the controls. NSE is a diagnostic marker of neuroendocrine tumors, brain injury and spinal cord trauma in humans. It seems that the NSE concentration in the CSF is increased by cellular destruction in canine encephalitis. Though elevation of NSE may not be specific in canine encephalitis because the NSE level was increased in other CNS diseases, further study including measurement with serum is necessary.
authors.affiliation: Department of Small Animal Clinical Sciences, School of Veterinary Medicine, Rakuno Gakuen University, 582 Bunkyodai-Midorimachi, Ebetsu, Hokkaido 069-8501, Japan.
authors.forename: Kozo
authors.initials: K
authors.lastname: Nakamura
authors.forename: Taku
authors.initials: T
authors.lastname: Miyasho
authors.forename: Sachiko
authors.initials: S
authors.lastname: Nomura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yokota
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Nakade
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000134:cerebrospinal fluid; D004283:Dog Diseases / Q000134:cerebrospinal fluid*; D004285:Dogs; D015180:Electrophoresis, Gel, Two-Dimensional / Q000662:veterinary; D015151:Immunoblotting / Q000662:veterinary; D008590:Meningoencephalitis / Q000134:cerebrospinal fluid / Q000662:veterinary*; D010751:Phosphopyruvate Hydratase / Q000235:genetics / Q000302:isolation & purification*; D020543:Proteome / Q000235:genetics*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000662:veterinary
chemical_list: D015415:Biomarkers; D020543:Proteome; D010751:Phosphopyruvate Hydratase