title: Traceability of plant diet contents in raw cow milk samples.
abstract: The use of molecular marker in the dairy sector is gaining large acceptance as a reliable diagnostic approach for food authenticity and traceability. Using a PCR approach, the rbcL marker, a chloroplast-based gene, was selected to amplify plant DNA fragments in raw cow milk samples collected from stock farms or bought on the Italian market. rbcL-specific DNA fragments could be found in total milk, as well as in the skimmed and the cream fractions. When the PCR amplified fragments were sent to sequence, the nucleotide composition of the chromatogram reflected the multiple contents of the polyphytic diet.
authors.affiliation: Institute of Agricultural Biology and Biotechnology, Italian National Research Council, Milan 20133, Italy. ponzoni@ibba.cnr.it
authors.forename: Elena
authors.initials: E
authors.lastname: Ponzoni
authors.forename: Francesco
authors.initials: F
authors.lastname: Mastromauro
authors.forename: Silvia
authors.initials: S
authors.lastname: Gianì
authors.forename: Diego
authors.initials: D
authors.lastname: Breviario
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D015415:Biomarkers; D002417:Cattle; D018744:DNA, Plant / Q000032:analysis* / Q000235:genetics; D004032:Diet / Q000662:veterinary*; D005260:Female; D008892:Milk / Q000737:chemistry*; D010944:Plants / Q000235:genetics*
chemical_list: D015415:Biomarkers; D018744:DNA, Plant