title: Successful vitrification of mouse ovaries using less-concentrated cryoprotectants with Supercool X-1000 supplementation.
abstract: The purpose of our study was to investigate the feasibility of using less-concentrated cryoprotectants supplemented with ice blocker Supercool X-1000 to vitrify ovarian tissues. Mouse ovaries were cryopreserved in different concentrations of vitrification solution alone or with Supercool X-1000, and fresh non-frozen ovaries were used as control. The proportions of morphological normality of follicles, normal GCs in follicular fluids and developing to blastocysts were higher in 12.5% ethylene glycol (EG) + 12.5% dimethylsulfoxide (DMSO) with Supercool X-1000 than those of treated in 10% EG + 10% DMSO or 15% EG + 15% DMSO alone or with Supercool X-1000. In conclusion, the inclusion of Supercool X-1000 in less-concentrated vitrification solution was effective to improve the efficiency and efficacy of cryopreservation of ovarian tissues.
authors.affiliation: Shandong Key Lab of Animal Disease Control and Breeding, Institute of Animal Husbandry and Veterinary Medicine, Shandong Academy of Agriculture Sciences, Jinan, Shandong, China.
authors.forename: Xiuwen
authors.initials: X
authors.lastname: Tan
authors.forename: Enliang
authors.initials: E
authors.lastname: Song
authors.forename: Xiaomu
authors.initials: X
authors.lastname: Liu
authors.forename: Guifen
authors.initials: G
authors.lastname: Liu
authors.forename: Haijian
authors.initials: H
authors.lastname: Cheng
authors.forename: Fachun
authors.initials: F
authors.lastname: Wan
mesh_terms: D000818:Animals; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D019855:Ethylene Glycol / Q000494:pharmacology; D005260:Female; D051379:Mice; D006080:Ovarian Follicle / Q000033:anatomy & histology; D010053:Ovary / Q000033:anatomy & histology; D058989:Vitrification
chemical_list: D003451:Cryoprotective Agents; D019855:Ethylene Glycol; D004121:Dimethyl Sulfoxide