title: Effect of hydroalcoholic extract from Copaifera langsdorffii leaves on urolithiasis induced in rats.
abstract: Copaifera langsdorffii Desf. commonly known as "copaíba", produce a commercially valuable oil-resin that is extensively used in folk medicine for anti-inflammatory, antimicrobial and antiseptic purposes. We have found the hydroalcoholic extract of this plant leaf has the potential to treat urolithiasis, a problem affecting ~7% of the population. To isolate the functional compounds C. langsdorffii leaves were dried, ground, and macerated in a hydroalcoholic solution 7:3 to produce a 16.8% crude extract after solvent elimination. Urolithiasis was induced by introduction of a calcium oxalate pellet (CaOx) into the bladders of adult male Wistar rats. The treated groups received the crude extract by oral gavage at 20 mg/kg body weight daily for 18 days. Extract treatment started 30 days after CaOx seed implantation. To monitor renal function sodium, potassium and creatinine concentrations were analyzed in urine and plasma, and were found to be in the normal range. Analyses of pH, magnesium, phosphate, calcium, uric acid, oxalate and citrate levels were evaluated to determine whether the C. langsdorffii extract may function as a stone formation prevention agent. The HPLC analysis of the extract identified flavonoids quercitrin and afzelin as the major components. Animals treated with C. langsdorffii have increased levels of magnesium and decreased levels of uric acid in urinary excretions. Treated animals have a significant decrease in the mean number of calculi and a reduction in calculi mass. Calculi taken from extract treated animals were more brittle and fragile than calculi from untreated animals. Moreover, breaking calculi from untreated animals required twice the amount of pressure as calculi from treated animals (6.90 ± 3.45 vs. 3.00 ± 1.51). The extract is rich in flavonoid heterosides and other phenolic compounds. Therefore, we hypothesize this class of compounds might contribute significantly to the observed activity.
authors.affiliation: Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Ana Paula S
authors.initials: AP
authors.lastname: Brancalion
authors.forename: Rejane B
authors.initials: RB
authors.lastname: Oliveira
authors.forename: João Paulo B
authors.initials: JP
authors.lastname: Sousa
authors.forename: Milton
authors.initials: M
authors.lastname: Groppo
authors.forename: Andresa A
authors.initials: AA
authors.lastname: Berretta
authors.forename: Marcio E
authors.initials: ME
authors.lastname: Barros
authors.forename: Mirian A
authors.initials: MA
authors.lastname: Boim
authors.forename: Jairo K
authors.initials: JK
authors.lastname: Bastos
mesh_terms: D000818:Animals; D002129:Calcium Oxalate; D007887:Fabaceae; D008297:Male; D010936:Plant Extracts / Q000627:therapeutic use*; D018515:Plant Leaves; D051381:Rats; D017208:Rats, Wistar; D052878:Urolithiasis / Q000188:drug therapy*
chemical_list: D010936:Plant Extracts; D002129:Calcium Oxalate