title: Prematurity in mice leads to reduction in nephron number, hypertension, and proteinuria.
abstract: The nephron number at birth is a quantitative trait that correlates inversely with the risk of hypertension and chronic kidney disease later in life. During kidney development, the nephron number is controlled by multiple factors including genetic, epigenetic, and environmental modifiers. Premature birth, which represents more than 12% of annual live births in the United States, has been linked to low nephron number and the development of hypertension later in life. In this report, we describe the development of a mouse model of prematurity-induced reduction of nephron number. Premature mice, delivered 1 and 2 days early, have 17.4 ± 2.3% (n = 6) and 23.6 ± 2% (n = 10) fewer nephrons, respectively, when compared with full-term animals (12,252 ± 571 nephrons/kidney, n = 10). After 5 weeks of age, the mice delivered 2 days premature show lower real-time glomerular filtration rate (GFR, 283 ± 13 vs 389 ± 26 μL/min). The premature mice also develop hypertension (mean arterial pressure [MAP], 134 ± 18 vs 120 ± 14 mm Hg) and albuminuria (286 ± 83 vs 176 ± 59 μg albumin/mg creatinine). This mouse model provides a proof of concept that prematurity leads to reduced nephron number and hypertension, and this model will be useful in studying the pathophysiology of prematurity-induced nephron number reductions and hypertension.
authors.affiliation: Department of Medicine, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA.
authors.forename: Cary
authors.initials: C
authors.lastname: Stelloh
authors.forename: Kenneth P
authors.initials: KP
authors.lastname: Allen
authors.forename: David L
authors.initials: DL
authors.lastname: Mattson
authors.forename: Alexandra
authors.initials: A
authors.lastname: Lerch-Gaggl
authors.forename: Sreenivas
authors.initials: S
authors.lastname: Reddy
authors.forename: Asraf
authors.initials: A
authors.lastname: El-Meanawy
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002585:Cesarean Section; D004195:Disease Models, Animal; D005260:Female; D005865:Gestational Age; D005921:Glomerulonephritis / Q000209:etiology; D006801:Humans; D006977:Hypertension, Renal / Q000209:etiology*; D007231:Infant, Newborn; D007234:Infant, Premature; D007676:Kidney Failure, Chronic / Q000209:etiology*; D051379:Mice; D009399:Nephrons / Q000196:embryology / Q000473:pathology; D011247:Pregnancy; D011507:Proteinuria / Q000209:etiology*