title: Mutation of a raft-targeting signal in the transmembrane region retards transport of influenza virus hemagglutinin through the Golgi.
abstract: Inclusion of proteins into membrane-rafts favours interactions required for virus assembly but has also been proposed to facilitate vesicular transport of proteins. The hemagglutinin (HA) of influenza virus contains a raft-targeting sequence in the outer leaflet of its transmembrane region. We report that its mutation enhances co-localization of HA with a cis-Golgi marker and retards Golgi-localized processing, such as acquisition of Endo-H resistant carbohydrates and proteolytic cleavage. In contrast, trimerization of the molecule in the ER and transport to the apical membrane were not affected. The second signal for raft-targeting, S-acylation at cytoplasmic cysteines, did not retard HA transport.
authors.affiliation: Immunology and Molecular Biology, Veterinary Medicine Faculty, Free University, Berlin, Germany.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Engel
authors.forename: Maren
authors.initials: M
authors.lastname: de Vries
authors.forename: Andreas
authors.initials: A
authors.lastname: Herrmann
authors.forename: Michael
authors.initials: M
authors.lastname: Veit
mesh_terms: D000818:Animals; D002460:Cell Line; D004285:Dogs; D005089:Exocytosis; D006056:Golgi Apparatus / Q000378:metabolism*; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D021962:Membrane Microdomains / Q000378:metabolism*; D009154:Mutation; D021382:Protein Sorting Signals / Q000235:genetics*; D021381:Protein Transport
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; D021382:Protein Sorting Signals