title: Microbial community characterization of an UASB treating increased organic loading rates of vitamin C biosynthesis wastewater.
abstract: The microbial community of a mesophilic lab-scale upflow anaerobic sludge blanket (UASB) reactor treating vitamin C biosynthesis wastewater at gradually elevated organic loading rates (OLRs) was characterized using 16S rDNA-based polymerase chain reaction-DGGE (denatured gradient gel electrophoresis) analysis. The DGGE fingerprints suggested that the elevated OLRs did not cause any significant changes in the microbial community. The predominant bacterial bands were affiliated with the Firmicutes (Clostridiales, four bands), Proteobacteria (Deltaproteobacteria, six bands), Bacteroidetes, and Synergistetes, respectively. All the archaeal bands were very similar to already known methanogenic species: Methanobacterium formicicum (two bands), Methanomethylovorans hollandica (one band) and Methanosaeta concilli (two bands), which belonged to the divisions Methanobacteria and Methanomicrobia, respectively.
authors.affiliation: Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, China. shirongjiu@iae.ac.cn
authors.forename: Rongjiu
authors.initials: R
authors.lastname: Shi
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Weichao
authors.initials: W
authors.lastname: Yang
authors.forename: Hui
authors.initials: H
authors.lastname: Xu
mesh_terms: D000693:Anaerobiosis; D001105:Archaea / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D001205:Ascorbic Acid; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors / Q000382:microbiology*; D058645:Denaturing Gradient Gel Electrophoresis; D005232:Fatty Acids, Volatile / Q000378:metabolism; D007220:Industrial Waste / Q000032:analysis; D008697:Methane / Q000378:metabolism; D009584:Nitrogen / Q000032:analysis; D010710:Phosphates / Q000032:analysis; D016133:Polymerase Chain Reaction; D019642:RNA, Archaeal / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000382:microbiology; D013431:Sulfates / Q000032:analysis; D014865:Waste Disposal, Fluid; D014873:Water Pollutants / Q000032:analysis
chemical_list: D005232:Fatty Acids, Volatile; D007220:Industrial Waste; D010710:Phosphates; D019642:RNA, Archaeal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D013431:Sulfates; D014873:Water Pollutants; D009584:Nitrogen; D008697:Methane; D001205:Ascorbic Acid