title: Donor preparation using pneumatic dissection in endothelial keratoplasty: DMEK or DSEK?
abstract: OBJECTIVE
  To determine if residual stroma remains on the Descemet membrane when pneumatic dissection is used to prepare donor tissue for endothelial keratoplasty.
  
  
  METHODS
  Pneumatic dissection using a posterior peripheral needle insertion was carried out on 5 eye bank corneas. Samples were then sent for histological analysis.
  
  
  RESULTS
  A thin layer of stroma remained on the Descemet membrane in all the samples. The average central stromal thickness was 12.4 μm (range 6.5-20.0 μm).
  
  
  CONCLUSIONS
  Endothelial keratoplasty using pneumatic dissection to prepare donor tissue should be considered a form of Descemet stripping endothelial keratoplasty and not Descemet membrane endothelial keratoplasty. If future studies show excellent visual results using pneumatic dissection for endothelial keratoplasty, then removal of all donor stroma may be unnecessary.
authors.affiliation: Department of Ophthalmology, Manchester Royal Eye Hospital, Manchester, United Kingdom. hamishmckee@gmail.com
authors.forename: Hamish D
authors.initials: HD
authors.lastname: McKee
authors.forename: Luciane C D
authors.initials: LC
authors.lastname: Irion
authors.forename: Fiona M
authors.initials: FM
authors.lastname: Carley
authors.forename: Vishal
authors.initials: V
authors.lastname: Jhanji
authors.forename: Arun K
authors.initials: AK
authors.lastname: Brahma
mesh_terms: D003319:Corneal Stroma / Q000601:surgery*; D057111:Descemet Stripping Endothelial Keratoplasty / Q000379:methods*; D004210:Dissection / Q000379:methods*; D004728:Endothelium, Corneal / Q000601:surgery* / Q000637:transplantation; D005125:Eye Banks; D006801:Humans; D013508:Ophthalmologic Surgical Procedures; D014019:Tissue Donors