title: Negative effects of ADAMTS-7 and ADAMTS-12 on endplate cartilage differentiation.
abstract: The roles of ADAMTS-7 and ADAMTS-12 in disc degeneration have not been previously examined. The purpose of this study was to examine the expression of ADAMTS-7 and ADAMTS-12 in the endplate cells isolated from patients with degenerative disc disease and to see whether they are associated with the pathological change of endplate. Sixty-four degenerated lumbar endplate specimens were obtained from the patients with degenerative disc disease categorized as type Modic I or II in magnetic resonance imaging (MRI) and 12 nondegenerative specimens as control (vertebra burst fracture patients without degenerative change in MRI) during surgical procedures. The expression of ADAMTS-7 and ADAMTS-12 was examined by real-time PCR and Western blotting. A statistically significant increase in mRNA expression of ADAMTS-7 and ADAMTS-12 was observed in the endplate cells in degenerative discs compared with nondegenerative discs. The corresponding protein levels of ADAMTS-7 and ADAMTS-12 had the same expression patterns. Moreover, ADAMTS-7 and ADAMTS-12 down-regulated the expression of Col II, Sox9, and Col X the marker genes for chondrogenesis. Our results indicate that ADAMTS-7 and ADAMTS-12 appear to be potent negative regulators of endplate cartilage development.
authors.affiliation: Department of Orthopaedics, General Hospital of Chinese People's Liberation Army, Beijing 100853, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhang
authors.forename: Ming
authors.initials: M
authors.lastname: Huang
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Wang
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Xu
authors.forename: Ming
authors.initials: M
authors.lastname: Ni
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
mesh_terms: D051722:ADAM Proteins / Q000096:biosynthesis*; D000071096:ADAMTS Proteins; D000071118:ADAMTS7 Protein; D000328:Adult; D002356:Cartilage / Q000378:metabolism*; D064236:Cartilage Oligomeric Matrix Protein; D002454:Cell Differentiation; D002460:Cell Line; D019902:Chondrocytes / Q000166:cytology; D024043:Collagen Type II / Q000096:biosynthesis; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis; D006023:Glycoproteins / Q000096:biosynthesis; D006801:Humans; D055959:Intervertebral Disc Degeneration / Q000378:metabolism*; D008159:Lumbar Vertebrae / Q000378:metabolism; D064235:Matrilin Proteins; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D064236:Cartilage Oligomeric Matrix Protein; D024043:Collagen Type II; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D064235:Matrilin Proteins; D012333:RNA, Messenger; C577553:TSP5 protein, human; D051722:ADAM Proteins; D000071096:ADAMTS Proteins; C496252:ADAMTS12 protein, human; D000071118:ADAMTS7 Protein; C121534:ADAMTS7 protein, human