title: Direct imaging of DNA motif sequences with encoded nanoparticles.
abstract: We present a method for encoded tagging and imaging of short nucleic acid motif chains (oligomotifs) using selective hybridization of heterogeneous Au nanoparticles. The resulting encoded nanoparticle string is thus representative of the underlying motif sequence. Since the nanoparticles are much more massive than the motifs; the motif chain order can be directly observed using scanning electron microscopy. Using this technique we demonstrate direct sequencing of oligomotifs in single DNA molecules consisting of four 100-bp motif chains tagged with four different types of nanoparticles. The method outlined is a precursor for a high density direct sequencing technology.
authors.affiliation: Department of Electrical and Computer Engineering, University of Utah, UT, USA. rennyedwin@gmail.com
authors.forename: Renny E
authors.initials: RE
authors.lastname: Fernandez
authors.forename: Carlos H
authors.initials: CH
authors.lastname: Mastrangelo
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D017931:DNA Primers; D053758:Nanoparticles; D009693:Nucleic Acid Hybridization
chemical_list: D017931:DNA Primers; D004247:DNA