title: Role of spinal cord in regulating mouse kidney: a virally mediated trans-synaptic tracing study.
abstract: OBJECTIVE
  To determine the spinal innervation and neuronal connections is important for studying renal metabolic responses.
  
  
  METHODS
  In this study, the spinal cords of 10 adult male C57BL/6J strain mice were mapped retrograde using injections of pseudorabies virus (PRV)-614. The virus, injected into the kidney, was specifically transported to the spinal cord.
  
  
  RESULTS
  At 5 days after injection of the PRV-614, PRV-614-positive cells were found in the intermediolateral cell column, the intercalates nucleus, or the central autonomic nucleus of spinal cord segments T4-L1, and most PRV-614-labeled cells were found in the T9 segment.
  
  
  CONCLUSIONS
  Our results revealed neuroanatomical circuits between kidney and the spinal intermediolateral cell column neurons.
authors.affiliation: Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
authors.forename: Da-Wei
authors.initials: DW
authors.lastname: Ye
authors.forename: Rong-Chun
authors.initials: RC
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.forename: Cheng
authors.initials: C
authors.lastname: Liu
authors.forename: Dong
authors.initials: D
authors.lastname: Ni
authors.forename: Qing-Bo
authors.initials: QB
authors.lastname: Huang
authors.forename: Xin
authors.initials: X
authors.lastname: Ma
authors.forename: Hong-Zhao
authors.initials: HZ
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Yang
authors.forename: Hong-Bing
authors.initials: HB
authors.lastname: Xiang
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001370:Axonal Transport / Q000502:physiology; D005456:Fluorescent Dyes / Q000378:metabolism; D011558:Herpesvirus 1, Suid; D007150:Immunohistochemistry; D007395:Interneurons / Q000502:physiology; D007668:Kidney / Q000294:innervation / Q000502:physiology*; D008164:Luminescent Proteins / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D056347:Neuroanatomical Tract-Tracing Techniques / Q000379:methods*; D013116:Spinal Cord / Q000502:physiology*; D013131:Spine / Q000294:innervation*; D000097573:Red Fluorescent Protein
chemical_list: D005456:Fluorescent Dyes; D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein