title: Transcriptomic analysis of Chinese bayberry (Myrica rubra) fruit development and ripening using RNA-Seq.
abstract: BACKGROUND
  Chinese bayberry (Myrica rubra Sieb. and Zucc.) is an important subtropical fruit crop and an ideal species for fruit quality research due to the rapid and substantial changes that occur during development and ripening, including changes in fruit color and taste. However, research at the molecular level is limited by a lack of sequence data. The present study was designed to obtain transcript sequence data and examine gene expression in bayberry developing fruit based on RNA-Seq and bioinformatic analysis, to provide a foundation for understanding the molecular mechanisms controlling fruit quality changes during ripening.
  
  
  RESULTS
  RNA-Seq generated 1.92 G raw data, which was then de novo assembled into 41,239 UniGenes with a mean length of 531 bp. Approximately 80% of the UniGenes (32,805) were annotated against public protein databases, and coding sequences (CDS) of 31,665 UniGenes were determined. Over 3,600 UniGenes were differentially expressed during fruit ripening, with 826 up-regulated and 1,407 down-regulated. GO comparisons between the UniGenes of these two types and interactive pathways (Ipath) analysis found that energy-related metabolism was enhanced, and catalytic activity was increased. All genes involved in anthocyanin biosynthesis were up-regulated during the fruit ripening processes, concurrent with color change. Important changes in carbohydrate and acid metabolism in the ripening fruit are likely associated with expression of sucrose phosphate synthase (SPS) and glutamate decarboxylase (GAD).
  
  
  CONCLUSIONS
  Mass sequence data of Chinese bayberry was obtained and the expression profiles were examined during fruit ripening. The UniGenes were annotated, providing a platform for functional genomic research with this species. Using pathway mapping and expression profiles, the molecular mechanisms for changes in fruit color and taste during ripening were examined. This provides a reference for the study of complicated metabolism in non-model perennial species.
authors.affiliation: Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Feng
authors.forename: Ming
authors.initials: M
authors.lastname: Chen
authors.forename: Chang-jie
authors.initials: CJ
authors.lastname: Xu
authors.forename: Lin
authors.initials: L
authors.lastname: Bai
authors.forename: Xue-ren
authors.initials: XR
authors.lastname: Yin
authors.forename: Xian
authors.initials: X
authors.lastname: Li
authors.forename: Andrew C
authors.initials: AC
authors.lastname: Allan
authors.forename: Ian B
authors.initials: IB
authors.lastname: Ferguson
authors.forename: Kun-song
authors.initials: KS
authors.lastname: Chen
mesh_terms: D000872:Anthocyanins / Q000096:biosynthesis / Q000235:genetics; D002681:China; D005638:Fruit / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism; D005968:Glutamate Decarboxylase / Q000235:genetics / Q000378:metabolism; D031641:Myrica / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development*; D017423:Sequence Analysis, RNA; D059467:Transcriptome
chemical_list: D000872:Anthocyanins; D005964:Glucosyltransferases; C022698:sucrose-phosphate synthase; D005968:Glutamate Decarboxylase