title: [The standard operating procedure of the standardization breeding for Poecilobdella manillensis].
abstract: OBJECTIVE
  To prepare Standard Operating Procedure (SOP) for the artificial breeding technology of Poecilobdella manillensi.
  
  
  METHODS
  The environment of producing area and breeding technology, the control of diseases, reserving seeds for breeding, harvesting and processing,the standard of the product quality and supervision for the standardization breeding of Poecilobdella Manillensis was studied.
  
  
  RESULTS
  The average yield of vital specimen of Poecilobdella Manillensis was 420.88 kg/mu, the natural hirudin in 1kg of the living specimen of Poecilobdella Manillensis contained 430,000 AT-U, and the quality index and hygienic standard of all products complied with the regulations and standards of the State.
  
  
  CONCLUSIONS
  SOP is applicable for the breeding of Poecilobdella manillensi for Guangxi district.
authors.affiliation: Institute of Biology, Guangxi Academy of Sciences, Nanning 530007, China. zhouweiguan@126.com
authors.forename: Wei-Guan
authors.initials: WG
authors.lastname: Zhou
authors.forename: Wei-Hai
authors.initials: WH
authors.lastname: Zhou
authors.forename: Yi
authors.initials: Y
authors.lastname: Qiu
authors.forename: Jian-Cong
authors.initials: JC
authors.lastname: Guan
mesh_terms: D000383:Agriculture / Q000379:methods / Q000592:standards; D000818:Animals; D001947:Breeding / Q000379:methods / Q000592:standards; D017753:Ecosystem; D004777:Environment; D007865:Leeches / Q000254:growth & development*; D008419:Materia Medica / Q000592:standards*; D010598:Pharmacognosy / Q000592:standards*; D011786:Quality Control; D012987:Soil; D014867:Water
chemical_list: D008419:Materia Medica; D012987:Soil; D014867:Water