title: Effect of oocyte quality on blastocyst development after in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) in a sheep model.
abstract: OBJECTIVE
  To compare blastocyst production, after IVF and ICSI, from sheep oocytes of various quality. Sham-injected oocytes and parthenogenetic activated oocyte groups were considered as control.
  
  
  METHODS
  Prospective experimental study.
  
  
  METHODS
  University.
  
  
  METHODS
  Three- to 6-month-old sheep.
  
  
  METHODS
  Oocyte quality was assessed with the use of brilliant cresyl blue (BCB) stain. Adenosine triphosphate content was measured. Intracytoplasmic sperm injection and IVF were performed and blastocyst development and cell numbers were analyzed.
  
  
  METHODS
  Adenosine triphosphate content, embryo development and blastomere numbers.
  
  
  RESULTS
  After IVF, BCB-stained (BCB+) oocytes developed up to the blastocyst stage at higher percentages and with more cells per embryo (24.1% vs 4.0% and 69.7 vs 43.9, respectively) than unstained (BCB-) oocytes. Using intracytoplasmic sperm injection, no differences were found in blastocyst production (14.3% vs 11.8%) and number of cells per embryo (71.1 vs 54.3) between BCB+ and BCB- oocytes. Adenosine triphosphate content was higher before in vitro maturation than after in both types of oocytes. Brilliant cresyl blue-stained oocytes had more adenosine triphosphate content than BCB- oocytes.
  
  
  CONCLUSIONS
  Brilliant cresyl blue-stained oocytes show more adenosine triphosphate content than BCB- oocytes. Results from IVF were affected by the oocyte quality while ICSI did not produce differences in embryo development or blastomere numbers.
authors.affiliation: Department of Animal and Food Science, Universitat Autònoma de Barcelona, Barcelona, Spain.
authors.forename: Maria G
authors.initials: MG
authors.lastname: Catalá
authors.forename: Dolors
authors.initials: D
authors.lastname: Izquierdo
authors.forename: Maria
authors.initials: M
authors.lastname: Rodríguez-Prado
authors.forename: Sondes
authors.initials: S
authors.lastname: Hammami
authors.forename: Maria-Teresa
authors.initials: MT
authors.lastname: Paramio
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001755:Blastocyst; D005260:Female; D005307:Fertilization in Vitro; D008297:Male; D023421:Models, Animal; D009865:Oocytes / Q000378:metabolism / Q000473:pathology*; D010312:Parthenogenesis; D012756:Sheep; D020554:Sperm Injections, Intracytoplasmic
chemical_list: D000255:Adenosine Triphosphate