title: [Experimental study on the functional regulation of naringin in human periodontal ligament cells].
abstract: OBJECTIVE
  To detect the effect of naringin on human periodontal ligament cells' (hPDLCs) proliferation,bone formation and OPG mRNA expression.
  
  
  METHODS
  hPDLCs were primarily cultured and identified in vitro through enzyme digestion combined tissue culture method. With 3-(4, 5-Dimethylthiazol-2- yl)-2, 5-diphenyltetrazolium bromide (MTT) method, enzyme linked immunosorbent assay and RT-PCR, the hPDLCs' proliferation, alkaline phosphatase (ALP) activity, expression of collagen protein-I and OPG mRNA were observed after treatment with different concentrations (100,10,1.0,0.1,0.01mg/L) of naringin at different times. SPSS16.0 software package was used for statistical analysis.
  
  
  RESULTS
  Primary cultured hPDLCs had good shape; Significant promotion of proliferation,ALP activity and collagen protein-I expression of hPDLCs with naringin was found at the dose of 1.0 mg/L; and 1.0mg/L naringin regulated the expression of OPG mRNA in time-dependent manner.
  
  
  CONCLUSIONS
  Naringin might significantly promote the proliferation of hPDLCs and conversion into the osteoblast.
authors.affiliation: Research Center of Stomatology, College of Stomatology, Xi'an Jiaotong University, Xi'an 710004, Shanxi Province, China. drliang@mail.xjtu.edu.cn
authors.forename: Ang
authors.initials: A
authors.lastname: Li
authors.forename: Jun-jie
authors.initials: JJ
authors.lastname: Zhao
authors.forename: Jin
authors.initials: J
authors.lastname: Liu
authors.forename: Jian-feng
authors.initials: JF
authors.lastname: Shi
authors.forename: Guo-zhou
authors.initials: GZ
authors.lastname: Rao
authors.forename: Hong
authors.initials: H
authors.lastname: Wei
authors.forename: Jian-zhong
authors.initials: JZ
authors.lastname: Gou
mesh_terms: D049109:Cell Proliferation; D002478:Cells, Cultured; D044950:Flavanones; D006801:Humans; D010006:Osteoblasts; D010513:Periodontal Ligament
chemical_list: D044950:Flavanones; C005274:naringin