title: Cytotoxicity evaluation of Curcuma zedoaria (Christm.) Roscoe fluid extract used in oral hygiene products.
abstract: OBJECTIVE
  This in vitro study evaluated the cytotoxic effects of the Curcuma zedoaria (Christm.) Roscoe (popular name: zedoary) fluid extract, as used in preparations for oral hygiene, mostly for anti-septic purposes.
  
  
  METHODS
  The cell viability and cell growth were assessed by Trypan blue dye exclusion assay using the LMF cell line derived from oral mucosa. Cell viability (short-term assay) was measured 0, 6, 12 and 24 h after contact with the fluid extract. Cell growth (long-term assay) was analyzed in 1, 3, 5 and 7 days. The experimental groups were those testing the fluid extract obtained from the zedoary rhizome and the extractor liquid (ethanol 70° GL) in the concentrations of 0.01-0.0001% v/v. Fresh DMEM were used in the control cultures.
  
  
  RESULTS
  Short-term assay-all studied cultures maintained stable cell viability; Long-term assay-there was progressive cell growth in all studied cultures.
  
  
  CONCLUSIONS
  According to the results, the zedoary fluid extract presents low cytotoxicity and probably can be used in the oral hygiene products.
authors.affiliation: Centro de Ciências Biológicas e da Saúde, Universidade Presbiteriana Mackenzie, SP, Brazil. joao.fernandes@mackenzie.br
authors.forename: Joao Paulo Dos Santos
authors.initials: JP
authors.lastname: Fernandes
authors.forename: Anna Carolina Volpi
authors.initials: AC
authors.lastname: Mello-Moura
authors.forename: Marcia Martins
authors.initials: MM
authors.lastname: Marques
authors.forename: Maria Aparecida
authors.initials: MA
authors.lastname: Nicoletti
mesh_terms: D002470:Cell Survival / Q000187:drug effects*; D030024:Curcuma / Q000737:chemistry*; D006801:Humans; D009910:Oral Hygiene; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D010936:Plant Extracts