title: Purification and characterization of a novel antimicrobial peptide from Brevibacillus laterosporus strain A60.
abstract: A novel antimicrobial peptide, with molecular mass of 1602.0469Da, produced by Brevibacillus laterosporus strain A60 was isolated and purified from the soil of mango plants. The purification procedure consisted of ammonium sulfate precipitation, cation exchange chromatography on an HiTrap SP HP column, thin layer chromatography and High Performance Liquid Chromatography (HPLC) on C18 reversed-phase column. After the four isolation procedures, one peptide with antimicrobial activity was obtained and named BL-A60. The determination of the complete amino acid sequences of this peptide showed that it contains eleven amino acid residues, L-Y-K-L-V-K-V-V-L-N-M, and a choline connected to the N-terminal and a tenuazonic acid modified of the C-terminal. This peptide shows relatively low identification to other antimicrobial peptides from bacteria. Purified BL-A60 showed high pH and thermal stability and a strong inhibition of different stages of the life cycle of Phytophthora capsici, including mycelial growth, sporangia formation and cystospore germination, with EC(50) values of 7.89, 0.60 and 21.96 μg ml(-1), respectively.
authors.affiliation: Key Laboratory of Integrated Pest Management in Crops, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No 12 Zhongguancun South Street, Beijing 100081, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.forename: Lihua
authors.initials: L
authors.lastname: Guo
authors.forename: Hongmei
authors.initials: H
authors.lastname: Zeng
authors.forename: Xiufen
authors.initials: X
authors.lastname: Yang
authors.forename: Jingjing
authors.initials: J
authors.lastname: Yuan
authors.forename: Huaixing
authors.initials: H
authors.lastname: Shi
authors.forename: Yehui
authors.initials: Y
authors.lastname: Xiong
authors.forename: Mingjia
authors.initials: M
authors.lastname: Chen
authors.forename: Lei
authors.initials: L
authors.lastname: Han
authors.forename: Dewen
authors.initials: D
authors.lastname: Qiu
mesh_terms: D000595:Amino Acid Sequence; D000890:Anti-Infective Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D058675:Brevibacillus / Q000737:chemistry / Q000302:isolation & purification*; D002794:Choline / Q000737:chemistry; D002852:Chromatography, Ion Exchange; D004355:Drug Stability; D005658:Fungi / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D008970:Molecular Weight; D025282:Mycelium / Q000187:drug effects; D010838:Phytophthora / Q000187:drug effects; D010935:Plant Diseases / Q000382:microbiology; D020539:Sequence Analysis, Protein; D013172:Spores, Fungal / Q000187:drug effects; D013720:Tenuazonic Acid / Q000737:chemistry
chemical_list: D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D013720:Tenuazonic Acid; D002794:Choline