title: [Histomorphometric analysis of articular cartilage and subchondral bone from primary osteoarthritic knees].
abstract: OBJECTIVE
  To investigate the pathologic characteristics of the articular cartilage and subchondral bone from osteoarthritic knees, and to compare the structural parameters of articular cartilage and subchondral bone between the medial and lateral tibial plateau, so as to determine the role of calcified zone and subchondral bone in the pathogenesis of osteoarthritis (OA).
  
  
  METHODS
  The tibial plateaus were taken from 30 patients undergoing total knee arthroplasty between October 2009 and May 2011. The subjects included 11 males and 19 females with an average age of 65.1 years (range, 55-78 years). The mean disease duration was 16.6 years (range, 10-25 years); the mean varus angle of the diseased knee was 9.3 degrees (range, 1-23 degrees). After gross observation, the cartilage-bone samples were taken out from the most weight-bearing regions in the internal areas of the medial and lateral plateaus. The decalcified paraffin-embedded sections were prepared and stained with HE and Safranin O/fast green for cartilage assessment (Mankin score), staging, and bone histomorphometry; the pathologic features of the cartilage and subchondral bone were also observed. The thickness of total articular cartilage (TAC), articular calcified cartilage (ACC), subchondral bone plate (SCP), and the trabecular bone volume (BV/TV) were measured by Image Pro Plus 6.0 imaging system, then the ratio of ACC/TAC was calculated.
  
  
  RESULTS
  Macroscopic results showed that articular cartilage degeneration was more severe in the medial plateau than in the lateral plateau; Mankin score of the medial plateau (12.4 +/- 1.1) was significantly higher than that of the lateral plateau (8.3 +/- 1.6) (t = 12.173, P = 0.000). In the 60 samples, 14 samples were at stage I, characterised by fissures within the superficial zone, duplicated tidemark, and thickened subchondral bone; 19 samples were at stage II, characterised by fissures extending into the deep zone, multiple subchondral bone resorption pits, and obviously thickened subchondral bone; and 27 samples were at stage III, characterised by full-thickness cartilage defects, endochondral ossification, and eburnated subchondral bone. The bone histomorphometric study showed that TAC thickness of the medial plateau was significantly lower than that of the lateral plateau (P < 0.05); the ratios of ACC/TAC, BV/TV, and SCP thickness of the medial plateau were significantly higher than those of the lateral plateau (P < 0.05). However, there was no significant difference in the ACC thickness between the medial and lateral plateaus (P > 0.05).
  
  
  CONCLUSIONS
  The calcified zone and subchondral bone may play an important role in the initiation and progression of OA.
authors.affiliation: Center for Joint Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Song
authors.forename: Liu
authors.initials: L
authors.lastname: Yang
authors.forename: Fuyou
authors.initials: F
authors.lastname: Wang
mesh_terms: D000368:Aged; D002356:Cartilage / Q000473:pathology; D002358:Cartilage, Articular / Q000473:pathology*; D005260:Female; D006801:Humans; D007719:Knee Joint / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D020370:Osteoarthritis, Knee / Q000473:pathology*