title: [Effect of overexpression of nicotinic acid phosphoribosyl transferase on succinic acid production in Escherichia coli NZN111].
abstract: Escherichia coli strain NZN111 is a promising candidate for the fermentative production of succinate. However, because lactate dehydrogenase and pyruvate formate lyase were inactivated in NZN111, this strain had an unbalanced NADH/NAD+ ratio and could not use glucose under anaerobic conditions. In this study, a recombinant strain E. coli NZN111/pTrc99a-pncB was constructed to overexpress the nicotinic acid phosphoribosyl transferase gene (pncB). Under anaerobic conditions with the addition of 0.5 mmol/L nicotinic acid and 0.3 mmol/L isopropyl beta-D-thiogalactopyranoside (IPTG), the specific nicotinic acid phosphoribosyl transferase (NAPRTase, EC 2.4.2.11) activity in the recombinant strain was 11-fold higher than that in E. coli NZN111, the concentration of NAD(H) was increased by 3.85-fold, especially the concentration of NAD+ was increased by 5.17-fold and NADH/NAD+ was decreased from 0.640 to 0.125. The recombinant strain regained the capability of growth and glucose utilization under anaerobic conditions.
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China.
authors.forename: Rongming
authors.initials: R
authors.lastname: Liu
authors.forename: Jiangfeng
authors.initials: J
authors.lastname: Ma
authors.forename: Liya
authors.initials: L
authors.lastname: Liang
authors.forename: Bing
authors.initials: B
authors.lastname: Xu
authors.forename: Guangming
authors.initials: G
authors.lastname: Wang
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Min
authors.initials: M
authors.lastname: Jiang
mesh_terms: D000123:Acetyltransferases / Q000235:genetics / Q000378:metabolism; D000693:Anaerobiosis; D004926:Escherichia coli / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D005285:Fermentation; D024861:Genetic Enhancement / Q000379:methods*; D005947:Glucose / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D054409:Nicotinamide Phosphoribosyltransferase / Q000096:biosynthesis* / Q000235:genetics; D019802:Succinic Acid / Q000378:metabolism*
chemical_list: D009243:NAD; D019802:Succinic Acid; D007770:L-Lactate Dehydrogenase; D000123:Acetyltransferases; C022560:formate C-acetyltransferase; D054409:Nicotinamide Phosphoribosyltransferase; D005947:Glucose