title: [Research for thermal stability of fructose, glucose, 5-hydroxymethyl-2-furfural during the process of refining honey of honeyed pill].
abstract: OBJECTIVE
  To research the thermal stability of Fructose, Glucose, 5-Hydroxymethyl-2-furfural (5-HMF) during the process of refining honey.
  
  
  METHODS
  The refined honey was placed under different temperature and time. High Performance of Liquid Chromatography (HPLC) coupled with Evaporative Light Scattering Detection (ELSD) were used for the determination of fructose and glucose content and HPLC-UV detector was used to detect the content of 5-HMF in refined honey.
  
  
  RESULTS
  The contents of fructose and glucose decreased meanwhile the amount of 5-HMF conversion increased with the temperature increasing and the time extension.
  
  
  CONCLUSIONS
  Temperature is the main factor and the content of fructose, glucose and 5-HMF has no significant change below 8 degrees C. Therefore, low-temperature method is recommended for the process of increasing the viscosity of honey.
authors.affiliation: Shanghai University of Traditional Chinese Medicine Engineering Research Center of Modern Preparation Technology of TCM, Ministry of Education, Shanghai 201203, China. xjc_1983@126.com
authors.forename: Jie-Chen
authors.initials: JC
authors.lastname: Xian
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
authors.forename: Yi
authors.initials: Y
authors.lastname: Feng
authors.forename: Yan-Long
authors.initials: YL
authors.lastname: Hong
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004355:Drug Stability; D005632:Fructose / Q000032:analysis* / Q000737:chemistry; D005662:Furaldehyde / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D005947:Glucose / Q000032:analysis* / Q000737:chemistry; D006722:Honey / Q000032:analysis; D006358:Hot Temperature; D010084:Oxidation-Reduction; D011786:Quality Control; D015203:Reproducibility of Results; D013678:Technology, Pharmaceutical / Q000379:methods; D013997:Time Factors; D014783:Viscosity
chemical_list: D005632:Fructose; C008046:5-hydroxymethylfurfural; D005662:Furaldehyde; D005947:Glucose