title: Inflammatory changes in adipose tissue enhance expression of GPR84, a medium-chain fatty acid receptor: TNFα enhances GPR84 expression in adipocytes.
abstract: In this study we aimed to identify the physiological roles of G protein-coupled receptor 84 (GPR84) in adipose tissue, together with medium-chain fatty acids (MCFAs), the specific ligands for GPR84. In mice, high-fat diet up-regulated GPR84 expression in fat pads. In 3T3-L1 adipocytes, co-culture with a macrophage cell line, RAW264, or TNFα remarkably enhanced GPR84 expression. In the presence of TNFα, MCFAs down-regulated adiponectin mRNA expression in 3T3-L1 adipocytes. Taken together, our results suggest that GPR84 emerges in adipocytes in response to TNFα from infiltrating macrophages and exacerbates the vicious cycle between adiposity and diabesity.
authors.affiliation: Department of Metabolic Medicine, Nagoya University Graduate School of Medicine, Nagoya, Japan. hnagasak@fujita-hu.ac.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nagasaki
authors.forename: Takaaki
authors.initials: T
authors.lastname: Kondo
authors.forename: Masahiro
authors.initials: M
authors.lastname: Fuchigami
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Hashimoto
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Sugimura
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Ozaki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Arima
authors.forename: Akira
authors.initials: A
authors.lastname: Ota
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Oiso
authors.forename: Yoji
authors.initials: Y
authors.lastname: Hamada
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism; D052242:Adiponectin / Q000235:genetics; D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D018920:Coculture Techniques; D003652:Decanoic Acids / Q000494:pharmacology; D005227:Fatty Acids / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D007249:Inflammation / Q000235:genetics* / Q000378:metabolism; D008024:Ligands; D008264:Macrophages / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000235:genetics* / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D052242:Adiponectin; C499919:Adipoq protein, mouse; D003652:Decanoic Acids; D005227:Fatty Acids; C507114:Gpr84 protein, mouse; D008024:Ligands; D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled; D014409:Tumor Necrosis Factor-alpha; C031071:decanoic acid