title: Enhanced alveolar bone loss in a model of non-invasive periodontitis in rice rats.
abstract: OBJECTIVE
  The rice rat (Oryzomys palustris) develops periodontitis-like lesions when fed a diet rich in sucrose and casein (H-SC). We aimed to establish whether this model can accurately mimic the development of human periodontitis.
  
  
  METHODS
  For this purpose, 28-day-old rice rats (15/group) were assigned to standard (STD) or H-SC diets and sacrificed after 6, 12, and 18 weeks. Jaws were processed for morphometric, histometric, histologic, histomorphometric, and micro-CT analyses.
  
  
  RESULTS
  We found a progressive increase in horizontal alveolar bone loss (ABL) with age in maxillae of rats fed the STD diet as determined by morphometry. The H-SC diet exacerbated horizontal ABL at the palatal surface at 12 and 18 weeks. Furthermore, increased vertical ABL was detected in mandibles and maxillae of rats fed the H-SC diet for 12 and/or 18 weeks by histometry and micro-CT. Remarkably, the H-SC diet significantly increased bone remodeling at the interproximal alveolar bone of mandibles from rats fed for 6 weeks, but not in those fed for longer periods.
  
  
  CONCLUSIONS
  These findings indicate that the H-SC diet induced a transient increase in alveolar bone remodeling, which is followed by ABL characteristic of moderate periodontitis.
authors.affiliation: Department of Physiological Sciences, University of Florida, Gainesville, FL 32610, USA. aguirrej@ufl.edu
authors.forename: J I
authors.initials: JI
authors.lastname: Aguirre
authors.forename: M P
authors.initials: MP
authors.lastname: Akhter
authors.forename: D B
authors.initials: DB
authors.lastname: Kimmel
authors.forename: J
authors.initials: J
authors.lastname: Pingel
authors.forename: X
authors.initials: X
authors.lastname: Xia
authors.forename: A
authors.initials: A
authors.lastname: Williams
authors.forename: M
authors.initials: M
authors.lastname: Jorgensen
authors.forename: K
authors.initials: K
authors.lastname: Edmonds
authors.forename: J Y
authors.initials: JY
authors.lastname: Lee
authors.forename: M K
authors.initials: MK
authors.lastname: Reinhard
authors.forename: A H
authors.initials: AH
authors.lastname: Battles
authors.forename: L
authors.initials: L
authors.lastname: Kesavalu
authors.forename: T J
authors.initials: TJ
authors.lastname: Wronski
mesh_terms: D016301:Alveolar Bone Loss / Q000209:etiology*; D000539:Alveolar Process / Q000000981:diagnostic imaging / Q000473:pathology; D000821:Animal Feed / Q000009:adverse effects; D000818:Animals; D002364:Caseins / Q000009:adverse effects; D019422:Dietary Sucrose / Q000009:adverse effects; D004195:Disease Models, Animal; D005260:Female; D008297:Male; D010518:Periodontitis / Q000209:etiology*; D016205:Sigmodontinae; D055114:X-Ray Microtomography
chemical_list: D002364:Caseins; D019422:Dietary Sucrose