title: Microsatellites in immune-relevant regions and their associations with Maedi-Visna and ovine pulmonary adenocarcinoma viral diseases.
abstract: Maedi-Visna (MV) and ovine pulmonary adenocarcinoma (OPA) are two retroviral diseases occurring worldwide that affect adult sheep. Differences in incidence, which may be related to sheep-rearing and housing choices, as well as to genetics, and disease progression have been reported for both diseases. In this work four microsatellites located in immune-relevant regions, the major histocompatibility complex (MHC) region, interferon-γ and interleukin-12p35, were genotyped to determine their association with disease progression. The analysed sample included Latxa sheep with and without OPA and MV-characteristic lesions in their lungs. The microsatellites in the MHC were the most diverse, while the ones located in the cytokines were the less polymorphic. In the case of IFN-γ the results suggested the presence of null alleles. Significant results were detected for several microsatellite alleles in the association analysis carried out by logistic regression. All statistical analyses included a flock effect adjustment to avoid false positives due to genetic structuration. MHC Class I microsatellite alleles OMHC1*205 and OMHC1*193 were associated with disease progression for Maedi and OPA, respectively. Moreover, MHC Class II microsatellite allele DRB2*275 was associated with presence of lesions in Maedi. Furthermore, the MHC microsatellites were combined for a bioinformatic haplotype inference with the PHASE software. In total, 73 haplotypes were detected, 18 of them in more than 6 animals. After standard and weighted logistic regression analysis, two of them were significantly associated with susceptibility: OMHC1*205-DRB2*271 for Maedi and OMHC1*193-DRB2*271 for OPA, both with the Class I microsatellite alleles associated in the marker by marker study. Although more extensive analyses are needed to disentangle the relationship between host genetics and disease, as far as we know this is the first study demonstrating a significant association between sheep MHC Class I microsatellite alleles and susceptibility to Maedi-Visna and OPA viral diseases.
authors.affiliation: Genetics, Physical Anthropology and Animal Physiology Dpt., Faculty of Science and Technology, University of the Basque Country UPV/EHU, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Larruskain
authors.forename: E
authors.initials: E
authors.lastname: Minguijón
authors.forename: I
authors.initials: I
authors.lastname: Arostegui
authors.forename: B
authors.initials: B
authors.lastname: Moreno
authors.forename: R A
authors.initials: RA
authors.lastname: Juste
authors.forename: B M
authors.initials: BM
authors.lastname: Jugo
mesh_terms: D000483:Alleles; D000818:Animals; D005787:Gene Frequency / Q000235:genetics; D005805:Genes, MHC Class I / Q000235:genetics / Q000276:immunology; D005802:Genes, MHC Class II / Q000235:genetics / Q000276:immunology; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006239:Haplotypes / Q000235:genetics; D007371:Interferon-gamma / Q000235:genetics / Q000276:immunology; D018664:Interleukin-12 / Q000235:genetics / Q000276:immunology; D008285:Major Histocompatibility Complex / Q000235:genetics* / Q000276:immunology; D018895:Microsatellite Repeats / Q000235:genetics* / Q000276:immunology; D011021:Pneumonia, Progressive Interstitial, of Sheep / Q000235:genetics* / Q000276:immunology; D011648:Pulmonary Adenomatosis, Ovine / Q000235:genetics* / Q000276:immunology; D012756:Sheep / Q000235:genetics / Q000276:immunology; D014790:Visna-maedi virus
chemical_list: D018664:Interleukin-12; D007371:Interferon-gamma