title: Chmp1A acts as a tumor suppressor gene that inhibits proliferation of renal cell carcinoma.
abstract: Renal cell carcinoma (RCC) is a highly malignant and often fatal disease of the kidney. Chmp1A is a member of the Endosomal Sorting Complex Required for Transport (ESCRT-III) family, and plays a role in the cytoplasm in sorting proteins to the multivesicular body (MVB). Chmp1A functions as a tumor suppressor gene and has been reported in pancreatic tumor cells. Here, we examined the expression level of Chmp1A in human RCC tissues and renal tumor cells by real-time quantitative RT-PCR and western blot. We found that the expression level of Chmp1A is significantly lower in RCC tissues and renal tumor cells compared with adjacent non-tumorous tissues and normal renal cells. Additionally, inhibition of Chmp1A expression by shRNA induced tumor formation in normal renal cells. However, inhibition of Chmp1A did not significantly affect tumor cell proliferation in vitro and tumor progression in vivo. Interestingly, overexpression of Chmp1A using a eukaryotic plasmid inhibited the proliferation of renal tumor cells in vitro and the growth of renal tumor in vivo. Thus, our results demonstrate that Chmp1A functions as a tumor suppressor gene in renal cells and may be a useful target for treatment of RCC.
authors.affiliation: State Key Laboratory of Safety Evaluation for New Drugs, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang, China.
authors.forename: Zhenqiang
authors.initials: Z
authors.lastname: You
authors.affiliation: State Key Laboratory of Safety Evaluation for New Drugs, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang, China.
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Xin
authors.affiliation: Sericulture Research Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute of Veterinary Medicine, Guangdong Academy of Agricultural Sciences, Guangzhou, Guangdong, China.
authors.forename: Junying
authors.initials: J
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Safety Evaluation for New Drugs, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang, China.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Safety Evaluation for New Drugs, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Safety Evaluation for New Drugs, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang, China.
authors.forename: Pansheng
authors.initials: P
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Safety Evaluation for New Drugs, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang, China.
authors.forename: Yunxiang
authors.initials: Y
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Safety Evaluation for New Drugs, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang, China.
authors.forename: Guochan
authors.initials: G
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Safety Evaluation for New Drugs, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang, China.
authors.forename: Lijiang
authors.initials: L
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Safety Evaluation for New Drugs, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang, China.
authors.forename: Liqiang
authors.initials: L
authors.lastname: Gu
authors.affiliation: State Key Laboratory of Safety Evaluation for New Drugs, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang, China.
authors.forename: Zhiqin
authors.initials: Z
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Safety Evaluation for New Drugs, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Han
authors.affiliation: State Key Laboratory of Safety Evaluation for New Drugs, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang, China. Electronic address: xuanyaoxian1954@163.com.
authors.forename: Yaoxian
authors.initials: Y
authors.lastname: Xuan
mesh_terms: D002292:Carcinoma, Renal Cell / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D056827:Endosomal Sorting Complexes Required for Transport / Q000235:genetics* / Q000378:metabolism; D055785:Gene Knockdown Techniques; D016153:Genes, Suppressor; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics*; D034741:RNA, Small Interfering / Q000494:pharmacology; D014162:Transfection; D033921:Vesicular Transport Proteins
chemical_list: C443709:CHMP1A protein, human; D056827:Endosomal Sorting Complexes Required for Transport; D034741:RNA, Small Interfering; D033921:Vesicular Transport Proteins