title: Quantum dot fluorescence characterizes the nanoscale organization of T cell receptors for antigen.
abstract: Changes in the clustering of surface receptors modulate cell responses to ligands. Hence, global measures of receptor clustering can be useful for characterizing cell states. Using T cell receptor for antigen as an example, we show that k-space image correlation spectroscopy of quantum dots blinking detects T cell receptor clusters on a scale of tens of nanometers and reports changes in clustering after T cell activation. Our results offer a general approach to the global analysis of lateral organization and receptor clustering in single cells, and can thus be applied when the cell type of interest is rare.
authors.affiliation: Biology Department, The Johns Hopkins University, Baltimore, Maryland, USA.
authors.forename: Sarah
authors.initials: S
authors.lastname: Boyle
authors.forename: David L
authors.initials: DL
authors.lastname: Kolin
authors.forename: Joan Glick
authors.initials: JG
authors.lastname: Bieler
authors.forename: Jonathan P
authors.initials: JP
authors.lastname: Schneck
authors.forename: Paul W
authors.initials: PW
authors.lastname: Wiseman
authors.forename: Michael
authors.initials: M
authors.lastname: Edidin
mesh_terms: D000818:Animals; D005453:Fluorescence; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008822:Mice, Transgenic; D053758:Nanoparticles / Q000737:chemistry*; D045663:Quantum Dots; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D013997:Time Factors
chemical_list: D011948:Receptors, Antigen, T-Cell