title: Selective uncoupling of individual mitochondria within a cell using a mitochondria-targeted photoactivated protonophore.
abstract: Depolarization of an individual mitochondrion or small clusters of mitochondria within cells has been achieved using a photoactivatable probe. The probe is targeted to the matrix of the mitochondrion by an alkyltriphenylphosphonium lipophilic cation and releases the protonophore 2,4-dinitrophenol locally in predetermined regions in response to directed irradiation with UV light via a local photolysis system. This also provides a proof of principle for the general temporally and spatially controlled release of bioactive molecules, pharmacophores, or toxins to mitochondria with tissue, cell, or mitochondrion specificity.
authors.affiliation: Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, 161 Cathedral Street, Glasgow G4 0RE, UK.
authors.forename: Susan
authors.initials: S
authors.lastname: Chalmers
authors.forename: Stuart T
authors.initials: ST
authors.lastname: Caldwell
authors.forename: Caroline
authors.initials: C
authors.lastname: Quin
authors.forename: Tracy A
authors.initials: TA
authors.lastname: Prime
authors.forename: Andrew M
authors.initials: AM
authors.lastname: James
authors.forename: Andrew G
authors.initials: AG
authors.lastname: Cairns
authors.forename: Michael P
authors.initials: MP
authors.lastname: Murphy
authors.forename: John G
authors.initials: JG
authors.lastname: McCarron
authors.forename: Richard C
authors.initials: RC
authors.lastname: Hartley
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D016503:Drug Delivery Systems; D008928:Mitochondria / Q000502:physiology*; D015394:Molecular Structure; D032389:Myocytes, Smooth Muscle / Q000187:drug effects / Q000502:physiology; D009942:Organometallic Compounds / Q000494:pharmacology; D009943:Organophosphorus Compounds / Q000737:chemistry*; D014466:Ultraviolet Rays
chemical_list: D009942:Organometallic Compounds; D009943:Organophosphorus Compounds; C110932:tetramethyl rhodamine ethyl ester