title: [Expression, purification and interaction of human leukocyte antigen F and cluster of differentiation 8alpha homodimers].
abstract: To obtain large quantity of human leukocyte antigen F (HLA-F) and cluster of differentiation 8alpha homodimers (CD8alphaalpha) proteins and to study their relationship, HLA-F and CD8alpha genes with rare codon in Escherichia coli were cloned using an N-terminal synonymous mutation method. High-efficiency expression protein inclusion bodies were acquired. The proteins were refolded using the dilution method and purified with gel-filtration and anion exchange chromatography. The results of gel-filtration and native-PAGE indicate that HLA-F interacts with CD8alphaalpha. This interaction may affect the binding between CD8alphaalpha and other MHC molecules to regulate immune responses. These results provide a basis for further research of HLA-F.
authors.affiliation: Institute of Microbiology, Chinese Academic of Sciences, Beijing 100101, China.
authors.forename: Kaihua
authors.initials: K
authors.lastname: Luo
authors.forename: Zheng
authors.initials: Z
authors.lastname: Fan
authors.forename: Hongbin
authors.initials: H
authors.lastname: Li
authors.forename: Yiwei
authors.initials: Y
authors.lastname: Liu
mesh_terms: D016827:CD8 Antigens / Q000096:biosynthesis / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D009154:Mutation; D054730:Protein Interaction Domains and Motifs; D055503:Protein Multimerization; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification
chemical_list: D016827:CD8 Antigens; C431698:CD8alpha antigen; C062341:HLA-F antigens; D015395:Histocompatibility Antigens Class I; D011994:Recombinant Proteins