title: The susceptibility of erythrocytes to oxidation during storage of blood: effects of melatonin and propofol.
abstract: OBJECTIVE
  We investigated the effects of melatonin and propofol in lipid peroxidation and antioxidant capacity of erythrocytes in stored bloods.
  
  
  METHODS
  Donated blood was taken into three citrate-phosphate-dextrose containing blood bags. One bag was used as control, the others were added either melatonin or propofol. Erythrocyte lipid peroxidation and antioxidant capacity and their sensitivity to in vitro oxidation were measured on days 0, 7, 14, 21 and 28.
  
  
  RESULTS
  In control group, erythrocyte malondialdehyde levels and sensitivity to in vitro oxidation were increased whereas glutathione, glutathione peroxidase and superoxide dismutase levels were decreased. Melatonin prevented malondialdehyde accumulation and preserved glutathione, glutathione peroxidase and superoxide dismutase levels. Propofol preserved glutathione and glutathione peroxidase levels but did not affect catalase and superoxide dismutase activities.
  
  
  CONCLUSIONS
  We showed that melatonin in stored blood could prevent lipid peroxidation and increase the resistance of erythrocytes to in vitro oxidation while propofol did not show such effects.
authors.affiliation: Department of Biochemistry, Yüzüncü Yıl University School of Medicine, Van, Turkey. mrseker@hotmail.com
authors.forename: Mehmet Ramazan
authors.initials: MR
authors.lastname: Şekeroğlu
authors.forename: Zübeyr
authors.initials: Z
authors.lastname: Huyut
authors.forename: Aydın
authors.initials: A
authors.lastname: Him
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D001793:Blood Preservation / Q000379:methods*; D002374:Catalase / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects*; D008315:Malondialdehyde / Q000378:metabolism; D008550:Melatonin / Q000494:pharmacology*; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism*; D015742:Propofol / Q000493:pharmacokinetics*; D013482:Superoxide Dismutase / Q000378:metabolism; D013997:Time Factors
chemical_list: D000975:Antioxidants; D008315:Malondialdehyde; D010088:Oxidoreductases; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione; D008550:Melatonin; D015742:Propofol