title: Protective effects of green and white tea against benzo(a)pyrene induced oxidative stress and DNA damage in murine model.
abstract: In the current investigation, the ameliorative effect of green tea (GT) and white tea (WT) against benzo(a)pyrene (BaP) induced oxidative stress and DNA damage has been studied in the livers and lungs of Balb/c mice. A single dose of BaP (125 mg/kg, b.w. orally) increased the levels of lipid peroxidation (LPO) and decreased endogenous antioxidants such as superoxide dismutase (SOD), glutahione reductase (GR), catalase (CAT), and glutathione (GSH) significantly. Pretreatment with GT and WT for 35 days before a single dose of BaP elevated the decreased activity of GR, SOD, and CAT in liver tissue and also tended to normalize the levels of GSH and LPO in both hepatic and pulmonary tissues. The percentage of DNA in comet tail and 8-hydroxy-2'-deoxyguanosine levels reflected the decreasing pattern of DNA damage from the BaP-treated group to the groups that received pretreatment with GT and WT. Our study concludes that both GT and WT are effective in combating BaP induced oxidative insult and DNA damage. However, WT was found to be more protective than GT with respect to CAT (only in the liver), percentage of DNA in comet tail (only in the lungs), GST activity, and GSH content in both the tissues.
authors.affiliation: Department of Zoology, Panjab University, Chandigarh, India.
authors.forename: Manoj
authors.initials: M
authors.lastname: Kumar
authors.forename: V L
authors.initials: VL
authors.lastname: Sharma
authors.forename: Amit
authors.initials: A
authors.lastname: Sehgal
authors.forename: Mridula
authors.initials: M
authors.lastname: Jain
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D000975:Antioxidants / Q000032:analysis; D001564:Benzo(a)pyrene / Q000494:pharmacology*; D002374:Catalase / Q000032:analysis; D004247:DNA / Q000032:analysis; D004249:DNA Damage / Q000187:drug effects*; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000032:analysis; D005978:Glutathione / Q000032:analysis; D005980:Glutathione Reductase / Q000032:analysis; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000737:chemistry / Q000378:metabolism; D008168:Lung / Q000737:chemistry / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018384:Oxidative Stress / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000032:analysis; D013662:Tea
chemical_list: D000975:Antioxidants; D013662:Tea; D001564:Benzo(a)pyrene; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D002374:Catalase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D003849:Deoxyguanosine; D005978:Glutathione