title: Role of stromal and Thy 1,2+ cells in the mechanisms of action of immobilized granulocyte colony-stimulating factor during cytostatic-induced myelosuppression.
abstract: The effects of immobilized granulocyte colony-stimulating factor (mediated by cells of the hemopoiesis-inducing microenvironment) on hemopoietic precursors of various classes were studied on the model of cytostatic-induced myelosuppression (administration of cyclophosphamide). The action of this preparation was compared with that of the standard preparation of granulocyte colony-stimulating factor. Thy 1,2(+)cells potentiated the effects of immobilized and standard granulocyte colony-stimulating factors on granulocyte-erythroid-macrophage-megakaryocyte precursors. Stromal cells were shown to potentiate the influence of these agents on granulocyte precursors. Induction of proliferation of precursor cells by the immobilized factor mediated by cells of the hemopoiesis-inducing microenvironment persisted for a longer period compared to that induced by the standard product.
authors.affiliation: Institute of Pharmacology, Siberian Division of Russian Academy of Medical Sciences, Tomsk, Russia.
authors.forename: T V
authors.initials: TV
authors.lastname: Andreeva
authors.forename: A V
authors.initials: AV
authors.lastname: Artamonov
authors.forename: A A
authors.initials: AA
authors.lastname: Bekarev
authors.forename: E I
authors.initials: EI
authors.lastname: Vereschagin
authors.forename: P G
authors.initials: PG
authors.lastname: Madonov
authors.forename: O V
authors.initials: OV
authors.lastname: Pershina
authors.forename: E G
authors.initials: EG
authors.lastname: Skurikhin
authors.forename: E S
authors.initials: ES
authors.lastname: Khmelevskaya
authors.forename: A M
authors.initials: AM
authors.lastname: Dygai
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation; D003520:Cyclophosphamide / Q000494:pharmacology; D041905:Erythroid Cells / Q000276:immunology; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D006098:Granulocytes / Q000276:immunology; D006410:Hematopoiesis / Q000187:drug effects; D006413:Hematopoietic System / Q000187:drug effects; D008264:Macrophages / Q000276:immunology; D008533:Megakaryocytes / Q000276:immunology; D051379:Mice; D008808:Mice, Inbred CBA; D017154:Stromal Cells / Q000276:immunology / Q000378:metabolism*; D018417:Th1 Cells / Q000276:immunology / Q000378:metabolism*; D018418:Th2 Cells / Q000276:immunology / Q000378:metabolism*
chemical_list: D016179:Granulocyte Colony-Stimulating Factor; D003520:Cyclophosphamide