title: Yeast ribosomal protein S3 possesses a β-lyase activity on damaged DNA.
abstract: Yeast ribosomal protein S3 has multifunctional activities that are involved in both protein translation and DNA repair. Here, we report that yeast Rps3p cleaves variously damaged DNA that contains not only AP sites and pyrimidine dimers but also 7,8-hydro-8-oxoguanine. This study also revealed that Rps3p has a β-lyase activity with a broad range of substrate specificity which cleaves phosphodiester bonds of UV or oxidatively damaged DNA substrates. Mutation analysis of the yeast Rps3 protein including introduction of domain deletions and residue replacements identified the residues Asp154 and Lys200 are important for the catalytic activity. In addition, the repair enzyme activity of yeast Rps3p was confirmed by complementation in xth, nfo Escherichia coli cells in which the DNA repair process is defective.
authors.affiliation: Radiation Health Research Institute, Korea Hydro & Nuclear Power Co., Ltd., Seoul, South Korea.
authors.forename: Ki Moon
authors.initials: KM
authors.lastname: Seong
authors.forename: Sang-Oun
authors.initials: SO
authors.lastname: Jung
authors.forename: Hag Dong
authors.initials: HD
authors.lastname: Kim
authors.forename: Hee Ju
authors.initials: HJ
authors.lastname: Kim
authors.forename: You-Jin
authors.initials: YJ
authors.lastname: Jung
authors.forename: Soo-Young
authors.initials: SY
authors.lastname: Choi
authors.forename: Joon
authors.initials: J
authors.lastname: Kim
mesh_terms: D004249:DNA Damage; D004260:DNA Repair / Q000235:genetics / Q000502:physiology*; D004271:DNA, Fungal / Q000378:metabolism; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005816:Genetic Complementation Test; D007700:Kinetics; D016297:Mutagenesis, Site-Directed; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D017384:Sequence Deletion; D013379:Substrate Specificity
chemical_list: D004271:DNA, Fungal; C501334:RPS3 protein, S cerevisiae; D011994:Recombinant Proteins; D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase