title: Brain chondroitin/dermatan sulfate, from cerebral tissue to fine structure: extraction, preparation, and fully automated chip-electrospray mass spectrometric analysis.
abstract: Chondroitin sulfate (CS) and dermatan sulfate (DS) glycosaminoglycans (GAGs) are covalently linked to proteins, building up a wide range of proteoglycans, with a prevalent expression in the extracellular matrix (ECM). In mammalian tissues, these GAG species are often found as hybrid CS/DS chains. Their structural diversity during chain elongation is produced by variability of sulfation in the repeating disaccharide units. In central nervous system, a large proportion of the ECM is composed of proteoglycans; therefore, CS/DS play a significant role in the functional diversity of neurons, brain development, and some brain diseases. A requirement for collecting consistent data on brain proteoglycan glycosylation is the development of adequate protocols for CS/DS extraction and detailed compositional and structure analysis. This chapter will present a strategy, which combines biochemical tools for brain CS/DS extraction, purification, and fractionation, with a modern analytical platform based on chip-nanoelectrospray multistage mass spectrometry (MS) able to provide information on the essential structural elements such as epimerization, chain length, sulfate content, and sulfation sites.
authors.affiliation: Department of Chemical and Biological Sciences, Aurel Vlaicu University of Arad, Arad, Romania.
authors.forename: Alina D
authors.initials: AD
authors.lastname: Zamfir
authors.forename: Corina
authors.initials: C
authors.lastname: Flangea
authors.forename: Alina
authors.initials: A
authors.lastname: Serb
authors.forename: Eugen
authors.initials: E
authors.lastname: Sisu
authors.forename: Leon
authors.initials: L
authors.lastname: Zagrean
authors.forename: Andreas
authors.initials: A
authors.lastname: Rizzi
authors.forename: Daniela G
authors.initials: DG
authors.lastname: Seidler
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D001923:Brain Chemistry; D002807:Chondroitin / Q000737:chemistry* / Q000302:isolation & purification*; D003871:Dermatan Sulfate / Q000737:chemistry* / Q000302:isolation & purification*; D006801:Humans; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D003871:Dermatan Sulfate; D002807:Chondroitin