title: The rice pentatricopeptide repeat protein RF5 restores fertility in Hong-Lian cytoplasmic male-sterile lines via a complex with the glycine-rich protein GRP162.
abstract: The cytoplasmic male sterility (CMS) phenotype in plants can be reversed by the action of nuclear-encoded fertility restorer (Rf) genes. The molecular mechanism involved in Rf gene-mediated processing of CMS-associated transcripts is unclear, as are the identities of other proteins that may be involved in the CMS-Rf interaction. In this study, we cloned the restorer gene Rf5 for Hong-Lian CMS in rice and studied its fertility restoration mechanism with respect to the processing of the CMS-associated transcript atp6-orfH79. RF5, a pentatricopeptide repeat (PPR) protein, was unable to bind to this CMS-associated transcript; however, a partner protein of RF5 (GRP162, a Gly-rich protein encoding 162 amino acids) was identified to bind to atp6-orfH79. GRP162 was found to physically interact with RF5 and to bind to atp6-orfH79 via an RNA recognition motif. Furthermore, we found that RF5 and GRP162 are both components of a restoration of fertility complex (RFC) that is 400 to 500 kD in size and can cleave CMS-associated transcripts in vitro. Evidence that a PPR protein interacts directly with a Gly-rich protein to form a subunit of the RFC provides a new perspective on the molecular mechanisms underlying fertility restoration.
authors.affiliation: Key Laboratory of the Ministry of Education for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Hu
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
authors.forename: Wenchao
authors.initials: W
authors.lastname: Huang
authors.forename: Gai
authors.initials: G
authors.lastname: Liu
authors.forename: Ya
authors.initials: Y
authors.lastname: Gao
authors.forename: Jianming
authors.initials: J
authors.lastname: Wang
authors.forename: Qi
authors.initials: Q
authors.lastname: Huang
authors.forename: Yanxiao
authors.initials: Y
authors.lastname: Ji
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Qin
authors.forename: Lei
authors.initials: L
authors.lastname: Wan
authors.forename: Renshan
authors.initials: R
authors.lastname: Zhu
authors.forename: Shaoqing
authors.initials: S
authors.lastname: Li
authors.forename: Daichang
authors.initials: D
authors.lastname: Yang
authors.forename: Yingguo
authors.initials: Y
authors.lastname: Zhu
mesh_terms: D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D051479:Plant Infertility / Q000235:genetics / Q000502:physiology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D010940:Plant Proteins