title: [Failed fertilization after ICSI: causes and countermeasures].
abstract: As a new technique for IVF, ICSI has a high rate of monospermy but cannot prevent all fertilization failures, of which the possible causes are poor oocytes or sperm, abnormal oocyte activation, and ICSI technical problems. Controlled ovarian hyperstimulation during IVF could increase the apoptosis of oocytes and subsequently induce fertilization failure. There are several methods for artificial activation in case of oocyte activation failure. Sperm-induced fertilization failure is mostly due to minute malformation of sperm, for which morphologically selective ICSI is generally employed. However, totally failed fertilization after ICSI remains a real challenge.
authors.affiliation: Family Planning Research Institute, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China. chengdanny@126.com
authors.forename: Dan
authors.initials: D
authors.lastname: Cheng
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Cui-Cui
authors.initials: CC
authors.lastname: Guo
authors.forename: Cheng-Liang
authors.initials: CL
authors.lastname: Xiong
mesh_terms: D005260:Female; D006801:Humans; D008297:Male; D009865:Oocytes; D020554:Sperm Injections, Intracytoplasmic; D013094:Spermatozoa; D017211:Treatment Failure