title: Motor neuron morphology estimation for its classification in the Drosophila brain.
abstract: Type-specific dendritic arborization patterns dictate synaptic connectivity and are fundamental determinants of neuronal function. We exploit the morphological stereotypy and relative simplicity of the Drosophila nervous system to model the diverse dendritic morphologies of individual motor neurons (MNs) to understand underlying principles of synaptic connectivity in a motor circuit. The genetic tractability of Drosophila allows us to label single MNs with green fluorescent protein (GFP) and serially reconstruct identifiable MNs in 3D with confocal microscopy. Our computational approach aims at the robust segmentation of the MN volumes and the simultaneous partitioning into their compartments, namely the soma, axon and dendrites. We use the idea of co-segmentation, where every image along the z-axis (depth) is clustered using information from 'neighboring' depths. As appearance we use a 3D extension of Haar features and for the shape we define an implicit representation of the segmentation domain.
authors.affiliation: Department of Computer and Information Science, Indiana University-Purdue University Indianapolis, USA. gavriil@cs.iupui.edu
authors.forename: Gavriil
authors.initials: G
authors.lastname: Tsechpenakis
authors.forename: Ruwan Egoda
authors.initials: RE
authors.lastname: Gamage
authors.forename: Michael D
authors.initials: MD
authors.lastname: Kim
authors.forename: Akira
authors.initials: A
authors.lastname: Chiba
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001921:Brain / Q000166:cytology*; D048430:Cell Shape; D016000:Cluster Analysis; D004331:Drosophila melanogaster / Q000166:cytology* / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000378:metabolism; D021621:Imaging, Three-Dimensional; D007814:Larva / Q000166:cytology / Q000378:metabolism; D008954:Models, Biological; D009046:Motor Neurons / Q000145:classification* / Q000166:cytology* / Q000378:metabolism; D012996:Solutions
chemical_list: D015415:Biomarkers; D012996:Solutions; D049452:Green Fluorescent Proteins