title: Developmental changes in the localization of protein kinase CK2 in non-diapause and diapause eggs of the silkworm, Bombyx mori.
abstract: To analyze the role of protein kinase CK2 (CK2) during early embryogenesis in non-diapause and diapause of the silkworm, the distribution and localization of Bombyx mori CK2 (BmCK2) were investigated by an immunohistochemical technique using antibodies against the α- and β-subunits of BmCK2. Both were localized in blastoderm cells of non-diapause and diapause eggs until 24 h after oviposition. More than 24 h after oviposition, however, the distribution of BmCK2 was different in non-diapause and diapause eggs. In non-diapause eggs, BmCK2 was mainly localized in yolk cells. In contrast, in diapause eggs, the localization was mainly observed in germ-band cells. Furthermore, we confirmed that the RNA helicase-like protein that was localized together with BmCK2 in non-diapause eggs was phosphorylated by BmCK2 in vitro. These data suggest that the role of BmCK2 is different in non-diapause and diapause eggs.
authors.affiliation: Laboratory of Biology, Department of General Studies, College of Humanities and Sciences, Nihon University, Sakurajosui 3-25-40, Setagaya-ku, Tokyo 156-8550, Japan. sawada@chs.nihon-u.ac.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sawada
authors.forename: Yumi
authors.initials: Y
authors.lastname: Yamahama
authors.forename: Takayuki
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Toru
authors.initials: T
authors.lastname: Togawa
authors.forename: Keisuke
authors.initials: K
authors.lastname: Mase
mesh_terms: D000818:Animals; D012831:Bombyx / Q000201:enzymology*; D047390:Casein Kinase II / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D010063:Ovum / Q000201:enzymology*; D021381:Protein Transport / Q000502:physiology*
chemical_list: D047390:Casein Kinase II