title: Heme oxygenase is involved in nitric oxide- and auxin-induced lateral root formation in rice.
abstract: Lateral root (LR) development performs the essential tasks of providing water, nutrients, and physical support to plants. Therefore, understanding the regulation of LR development is of agronomic importance. In this study, we examined the effect of nitric oxide (NO), auxin, and hemin (Hm) on LR formation in rice. Treatment with Hm [a highly effective heme oxygenase (HO) inducer], sodium nitroprusside (SNP, an NO donor), or indole-3-butyric acid (IBA, a naturally occurring auxin) induced LR formation and HO activity. LR formation and HO activity induced by SNP and IBA but not Hm was reduced by the specific NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide. As well, Hm, SNP, and IBA could induce OsHO1 mRNA expression. Zn protoporphyrin IX (the specific inhibitor of HO) and hemoglobin (the carbon monoxide/NO scavenger) reduced LR number and HO activity induced by Hm, SNP, and IBA. Our data suggest that HO is required for Hm-, auxin-, and NO-induced LR formation in rice.
authors.affiliation: Department of Agronomy, National Taiwan University, Taipei, Taiwan, ROC.
authors.forename: Yi-Hsuan
authors.initials: YH
authors.lastname: Chen
authors.forename: Yun-Yang
authors.initials: YY
authors.lastname: Chao
authors.forename: Yun Yen
authors.initials: YY
authors.lastname: Hsu
authors.forename: Chwan-Yang
authors.initials: CY
authors.lastname: Hong
authors.forename: Ching Huei
authors.initials: CH
authors.lastname: Kao
mesh_terms: D001565:Benzoates / Q000494:pharmacology; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D006419:Heme Oxygenase (Decyclizing) / Q000235:genetics / Q000378:metabolism*; D006427:Hemin / Q000494:pharmacology; D006454:Hemoglobins / Q000494:pharmacology; D007093:Imidazoles / Q000494:pharmacology; D007210:Indoleacetic Acids / Q000494:pharmacology*; D007211:Indoles / Q000494:pharmacology; D009569:Nitric Oxide / Q000494:pharmacology*; D009599:Nitroprusside / Q000494:pharmacology; D012275:Oryza / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D011524:Protoporphyrins / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D036226:Seedlings / Q000187:drug effects / Q000201:enzymology
chemical_list: D001565:Benzoates; D006454:Hemoglobins; D007093:Imidazoles; D007210:Indoleacetic Acids; D007211:Indoles; D010940:Plant Proteins; D011524:Protoporphyrins; D012333:RNA, Messenger; C014612:indolebutyric acid; C079393:1,3-dihydroxy-4,4,5,5-tetramethyl-2-(4-carboxyphenyl)tetrahydroimidazole; C017803:zinc protoporphyrin; D009599:Nitroprusside; D009569:Nitric Oxide; D006427:Hemin; D006419:Heme Oxygenase (Decyclizing)