title: Actinidin, a protease from kiwifruit, induces changes in morphology and adhesion of T84 intestinal epithelial cells.
abstract: Actinidin belongs to the papain-like family of cysteine proteases and is a major kiwifruit allergen. In this study, the effect of actinidin on cellular morphology and adhesion of T84 intestinal cells was investigated. Both rounding and detachment of T84 cells were observed upon actinidin treatment. The morphological changes and cell desquamation was protease-dependent, as well as time- and concentration-dependent. Changes of intercellular adhesion and adhesion of epithelial cells to collagen upon actinidin treatment could be responsible for the cell rounding and give rise to discontinuous breaches in the epithelial monolayer observed in this study. Actinidin's action on cell morphology, adhesion and monolayer integrity were not due to compromised viability of T84 epithelial cells, as confirmed by MTT assay and flow cytometric analysis of the cell cycle. Damage to the epithelial monolayer of the intestine induced by actinidin should be further evaluated as an important factor in the development of kiwifruit allergy and other intestinal disorders.
authors.affiliation: Department of Experimental Oncology, Institute for Oncology and Radiology of Serbia, Pasterova 14, 11000 Belgrade, Serbia.
authors.forename: Milena
authors.initials: M
authors.lastname: Čavić
authors.forename: Milica
authors.initials: M
authors.lastname: Grozdanović
authors.forename: Aleksandar
authors.initials: A
authors.lastname: Bajić
authors.forename: Tatjana
authors.initials: T
authors.lastname: Srdić-Rajić
authors.forename: Pavle R
authors.initials: PR
authors.lastname: Anđjus
authors.forename: Marija
authors.initials: M
authors.lastname: Gavrović-Jankulović
mesh_terms: D029042:Actinidia / Q000201:enzymology*; D002448:Cell Adhesion / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D003546:Cysteine Endopeptidases / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D007413:Intestinal Mucosa / Q000187:drug effects*
chemical_list: D003546:Cysteine Endopeptidases; C018745:actinidain