title: Integrins establish dendrite-substrate relationships that promote dendritic self-avoidance and patterning in drosophila sensory neurons.
abstract: Dendrites achieve characteristic spacing patterns during development to ensure appropriate coverage of territories. Mechanisms of dendrite positioning via repulsive dendrite-dendrite interactions are beginning to be elucidated, but the control, and importance, of dendrite positioning relative to their substrate is poorly understood. We found that dendritic branches of Drosophila dendritic arborization sensory neurons can be positioned either at the basal surface of epidermal cells, or enclosed within epidermal invaginations. We show that integrins control dendrite positioning on or within the epidermis in a cell autonomous manner by promoting dendritic retention on the basal surface. Loss of integrin function in neurons resulted in excessive self-crossing and dendrite maintenance defects, the former indicating a role for substrate interactions in self-avoidance. In contrast to a contact-mediated mechanism, we find that integrins prevent crossings that are noncontacting between dendrites in different three-dimensional positions, revealing a requirement for combined dendrite-dendrite and dendrite-substrate interactions in self-avoidance.
authors.affiliation: Department of Physiology and Cellular Biophysics, Columbia University Medical Center, 630 W. 168th St. P&S 12-403, New York, NY 10032, USA.
authors.forename: Michelle E
authors.initials: ME
authors.lastname: Kim
authors.forename: Brikha R
authors.initials: BR
authors.lastname: Shrestha
authors.forename: Richard
authors.initials: R
authors.lastname: Blazeski
authors.forename: Carol A
authors.initials: CA
authors.lastname: Mason
authors.forename: Wesley B
authors.initials: WB
authors.lastname: Grueber
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D019521:Body Patterning / Q000235:genetics / Q000502:physiology*; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism; D003712:Dendrites / Q000502:physiology* / Q000648:ultrastructure; D004330:Drosophila; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D000078404:Epidermal Cells; D004817:Epidermis / Q000502:physiology / Q000648:ultrastructure; D005109:Extracellular Matrix / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics; D006735:Horseradish Peroxidase / Q000378:metabolism; D016023:Integrins / Q000235:genetics / Q000378:metabolism*; D007814:Larva; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D008954:Models, Biological; D009024:Morphogenesis; D012679:Sense Organs / Q000166:cytology; D011984:Sensory Receptor Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: D015815:Cell Adhesion Molecules; D029721:Drosophila Proteins; D016023:Integrins; D049452:Green Fluorescent Proteins; D006735:Horseradish Peroxidase