title: Quantity and proliferation rate of mesenchymal stem cells in human cord blood during gestation.
abstract: UCB (umbilical cord blood) as a resource of MSCs (mesenchymal stem cells) is widely accepted, but the quantity and characteristics of UCB-MSCs from different gestational ages have not been well studied. We have quantified the number of MSCs in UCB at different gestational ages using a multi-colour flowcytometer and compared the cell proliferation rates of these UCB-MSCs. Defining MSCs as CD44+/CD105+/CD34-/CD45 population, their numbers declined in the UCB at the gestational age. Proliferation rates were significantly higher in UCB before term than at full term. Non-full term UCB samples may be a better source of MSCs.
authors.affiliation: Key Laboratory of Cell Biology of China Ministry of Public Health, Department of Stem Cells and Regenerative Medicine, China Medical University, Shen Yang, Peoples Republic of China.
authors.forename: Lan-Lan
authors.initials: LL
authors.lastname: Ma
authors.forename: Fan-Biao
authors.initials: FB
authors.lastname: Meng
authors.forename: Ping
authors.initials: P
authors.lastname: Shi
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.forename: Xi-Ning
authors.initials: XN
authors.lastname: Pang
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000032:analysis; D002452:Cell Count; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D002478:Cells, Cultured; D002585:Cesarean Section; D005260:Female; D005312:Fetal Blood / Q000166:cytology* / Q000502:physiology; D005333:Fetus; D005434:Flow Cytometry; D005865:Gestational Age; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D011247:Pregnancy / Q000097:blood*
chemical_list: D015703:Antigens, CD