title: Simultaneous determination of chloramphenicol and florfenicol in liquid milk, milk powder and bovine muscle by LC-MS/MS.
abstract: A validated method based on European and Brazilian legislation is reported. It is applicable to the simultaneous determination of chloramphenicol (CAP) and florfenicol (FF) by LC-MS/MS in liquid milk, milk powder and bovine muscle. The chromatographic analysis is completed in 6 min and the extraction procedure is very simple, involving only one step liquid-extraction with ethyl acetate. Where it proved necessary to include clean-up, an efficient and rapid step using C18-dispersive solid was added. Initially, a complete validation was performed with liquid milk matrix; later the scope was extended to the other matrices through extending the inter-day precision (within laboratory reproducibility) RSD values. An internal standard (d(5)-CAP) was employed for quantitative purposes. The method was shown to have good accuracy and precision for determining CAP residues at the level of 0.3-0.6 g kg(-1) and FF residues at the level of 5-15 µg kg(-1).
authors.affiliation: Laboratory of Residues and Contaminants-LANAGRO-GO, Ministry of Agriculture, Livestock and Food Supply, Goiânia, Goiás, Brazil CEP 74674-025.
authors.forename: D R
authors.initials: DR
authors.lastname: Rezende
authors.forename: N Fleury
authors.initials: NF
authors.lastname: Filho
authors.forename: G L
authors.initials: GL
authors.lastname: Rocha
mesh_terms: D000818:Animals; D001938:Brazil; D002417:Cattle; D002701:Chloramphenicol / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis; D005506:Food Contamination / Q000032:analysis* / Q000706:statistics & numerical data; D057230:Limit of Detection; D008460:Meat / Q000032:analysis; D008892:Milk / Q000737:chemistry; D009132:Muscles / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013839:Thiamphenicol / Q000031:analogs & derivatives* / Q000032:analysis; D019155:Veterinary Drugs / Q000032:analysis
chemical_list: D019155:Veterinary Drugs; D002701:Chloramphenicol; C035534:florfenicol; D013839:Thiamphenicol