title: Downregulation of Id1 by small interfering RNA in gastric cancer inhibits             cell growth via the Akt pathway.
abstract: Inhibitor of differentiation or DNA binding (Id1) is a member of the helix-loop-helix             transcription factor family that is overexpressed in various types of cancer,             including gastric carcinoma. Previous studies showed that Id1 is a prognostic             marker in patients with gastric cancer. However, the role of Id1 in the proliferation             of human gastric cancer cells has yet to be clarified. In the present study, we             downregulated the Id1 gene in SGC-7901 gastric cancer cells by RNA interference,             and we also constructed a recombinant plasmid-expressing Id1 to investigate its             effects on the proliferation of SGC-7901 cells. Results showed that the downregulation             of Id1 inhibited proliferation of SGC-7901 cells, while the upregulation of Id1             had no effect on SGC-7901 cell proliferation. The potential mechanism was also             investigated. The changes of certain proteins associated with cell proliferation,             apoptosis and the cell cycle were detected by western blotting. Furthermore, we             demonstrated a positive correlation between Id1 and phospho-Akt expression in             SGC-7901 cells.
authors.affiliation: Department of Biochemistry and Molecular Biology, Zhongshan Medical College, Sun Yat-Sen University, Guangzhou, Guangdong 510080, PR China.
authors.forename: Guang
authors.initials: G
authors.lastname: Yang
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jianjun
authors.initials: J
authors.lastname: Xiong
authors.forename: Jing
authors.initials: J
authors.lastname: Wu
authors.forename: Changfu
authors.initials: C
authors.lastname: Yang
authors.forename: Hongbing
authors.initials: H
authors.lastname: Huang
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Zhu
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis; D002455:Cell Division; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D015536:Down-Regulation; D016195:G2 Phase; D006801:Humans; D051798:Inhibitor of Differentiation Protein 1 / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000494:pharmacology*; D015398:Signal Transduction; D013274:Stomach Neoplasms / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; C497453:ID1 protein, human; D051798:Inhibitor of Differentiation Protein 1; D034741:RNA, Small Interfering; D051057:Proto-Oncogene Proteins c-akt