title: TGFβ-inducible early gene-1 (TIEG1) mutations in hypertrophic cardiomyopathy.
abstract: Hypertrophic cardiomyopathy (HCM) is the most common heritable cardiovascular disease. A recent study showed that male KLF10-encoded TGFβ Inducible Early Gene-1 knock-out mice (TIEG-/-) develop HCM with 13-fold up-regulation of PTTG1-encoded pituitary tumor-transforming gene 1. We hypothesized TIEG1 could be a novel candidate gene in the pathogenesis of genotype negative HCM in humans, possibly through a loss of its repression on PTTG1 expression. A cohort of 923 unrelated patients from two independent HCM centers was analyzed for mutations in TIEG's four translated exons using DHPLC and direct DNA-sequencing. Site directed mutagenesis was performed to clone novel variants. The effect of TIEG1 mutations on SMAD7 and PTTG1 promoters was studied using transient transfection and luciferase-assays. Altered expression of PTTG1 in cardiac tissue was studied by immunohistochemistry (IHC) to determine levels of PTTG1 protein in hypertrophic diseases. Six novel TIEG1 missense mutations were discovered in six patients (two males/four females, mean age at diagnosis 56.2±23 years, MLVWT 20.8±4 mm). Compared to WT TIEG1, five TIEG1 mutants significantly increased PTTG1 promoter function similar to TIEG1-/--mice. By IHC, PTTG1-protein expression was significantly increased in multiple models of hypertrophic cardiac disease, including TIEG1-mutation positive HCM compared to normal hearts. This is the first article to associate mutations in TIEG1 to human disease with the discovery of six novel, HCM-associated variants. Functional assays suggest a role for PTTG1 in the pathogenesis of TIEG1-mediated HCM. Up-regulation of PTTG1 seems to be a common pathway in hypertrophic heart disease, including TIEG1-mediated HCM.
authors.affiliation: Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Rochester, Minnesota 55905, USA.
authors.forename: J Martijn
authors.initials: JM
authors.lastname: Bos
authors.forename: Malayannan
authors.initials: M
authors.lastname: Subramaniam
authors.forename: John R
authors.initials: JR
authors.lastname: Hawse
authors.forename: Imke
authors.initials: I
authors.lastname: Christiaans
authors.forename: Nalini M
authors.initials: NM
authors.lastname: Rajamannan
authors.forename: Joseph J
authors.initials: JJ
authors.lastname: Maleszewski
authors.forename: William D
authors.initials: WD
authors.lastname: Edwards
authors.forename: Arthur A M
authors.initials: AA
authors.lastname: Wilde
authors.forename: Thomas C
authors.initials: TC
authors.lastname: Spelsberg
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Ackerman
mesh_terms: D001483:Base Sequence; D002312:Cardiomyopathy, Hypertrophic / Q000235:genetics* / Q000473:pathology; D051765:Early Growth Response Transcription Factors / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D051741:Kruppel-Like Transcription Factors / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D020125:Mutation, Missense; D009363:Neoplasm Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D064249:Securin; D017422:Sequence Analysis, DNA; D051906:Smad7 Protein / Q000235:genetics*; D016212:Transforming Growth Factor beta / Q000235:genetics
chemical_list: D051765:Early Growth Response Transcription Factors; C098111:KLF10 protein, human; D051741:Kruppel-Like Transcription Factors; D009363:Neoplasm Proteins; C497806:SMAD7 protein, human; D064249:Securin; D051906:Smad7 Protein; D016212:Transforming Growth Factor beta; C578002:pituitary tumor-transforming protein 1, human