title: Differential effects of intravenous anesthetics on hepatosplanchnic microcirculation in rats: sidestream dark-field imaging study.
abstract: This study aimed to investigate the effects of intravenous anesthetics on hepatosplanchnic microcirculation in laparotomized mechanically ventilated rats using Sidestream Dark-field (SDF) imaging. Thirty male Wistar rats were divided into 5 groups (n = 6 each). All rats were initially anesthetized with 60 mg/kg pentobarbital (i.p.) for instrumentation. This was followed by either ketamine, propofol, thiopental, midazolam or saline+fentanyl (iv bolus over 5 min and then maintenance over 90 min). SDF imaging of the liver and distal ileum microcirculation was performed at the baseline and at t = 5, 35, 65 and 95 min. In propofol group there was increase of functional sinusoidal density (FSD) following induction (+25%, P < 0.05) and maintenance at t = 95 min (+10.3%, P < 0.05), in ketamine and midazolam group decrease of FSD was observed after induction (-20.4%, P < 0.05; -10.1%, P < 0.05) and during maintenance at t = 65 min (-11.6%, P < 0.05; -11.4%, P < 0.05) when compared to baseline. Following induction with propofol functional capillary density (FCD) of ileal longitudinal muscle layer increased (+10.6%, P < 0.05) and returned to baseline values during maintenance. Ketamine and midazolam decreased FCD of longitudinal layer after induction (-24.6%, P < 0.05; -21.1%, P < 0.05) and remained decreased during maintenance at t = 95 min (-10.8%, P < 0.05; -15.5%, P < 0.05). In thiopental and control group, changes in microcirculatory parameters were not significant throughout the study. In conclusion, intravenous anesthetics affect the hepatosplanchnic microcirculation differentially, propofol has shown protective effect on the liver and intestinal microcirculation.
authors.affiliation: Department of Anesthesiology and Intensive Care Medicine, University Hospital Hradec Kralove, Charles University in Prague, Faculty of Medicine in Hradec Kralove, Hradec Kralove, Czech Republic. turek@fnhk.cz
authors.forename: Zdenek
authors.initials: Z
authors.lastname: Turek
authors.forename: Christian
authors.initials: C
authors.lastname: Lehmann
authors.forename: Renata
authors.initials: R
authors.lastname: Parizkova
authors.forename: Jindrich
authors.initials: J
authors.lastname: Samek
authors.forename: Milan
authors.initials: M
authors.lastname: Kaska
authors.forename: Vladimir
authors.initials: V
authors.lastname: Cerny
mesh_terms: D018686:Anesthetics, Intravenous / Q000494:pharmacology*; D000818:Animals; D001784:Blood Gas Analysis; D005283:Fentanyl / Q000494:pharmacology; D007082:Ileum / Q000098:blood supply* / Q000187:drug effects; D007649:Ketamine / Q000494:pharmacology; D008099:Liver / Q000098:blood supply* / Q000187:drug effects; D008297:Male; D008833:Microcirculation / Q000187:drug effects*; D008874:Midazolam / Q000494:pharmacology; D015742:Propofol / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013874:Thiopental / Q000494:pharmacology
chemical_list: D018686:Anesthetics, Intravenous; D007649:Ketamine; D013874:Thiopental; D008874:Midazolam; D005283:Fentanyl; D015742:Propofol