title: Expression of GSK-3β in renal allograft tissue and its significance in pathogenesis of chronic allograft dysfunction.
abstract: OBJECTIVE
  To explore the expression of Glycogen synthase kinase 3 beta (GSK-3β) in renal allograft tissue and its significance in the pathogenesis of chronic allograft dysfunction.
  
  
  METHODS
  Renal allograft biopsy was performed in all of the renal allograft recipients with proteinuria or increased serum creatinine level who came into our hospital from January 2007 to December 2009. Among them 28 cases was diagnosed as chronic allograft dysfunction based on pahtological observation, including 21 males with a mean age of 45 ± 10 years old and 7 females with a mean age of 42 ± 9 years old. The time from kidney transplantation to biopsy were 1-9 (3.5) years. Their serum creatinine level were 206 ± 122 umol/L. Immunohistochemical assay and computer-assisted genuine color image analysis system (imagepro-plus 6.0) were used to detect the expression of GSK-3β in the renal allografts of 28 cases of recipients with chronic allograft dysfunction. Mean area and mean integrated optical density of GSK-3β expression were calculated. The relationship between expression level of GSK-3β and either the grade of inflammatory cell infiltration or interstitial fibrosis/tubular atrophy in renal allograft was analyzed. Five specimens of healthy renal tissue were used as controls.
  
  
  RESULTS
  The expression level of the GSK-3β was significantly increased in the renal allograft tissue of recipients with chronic allograft dysfunction, compared to normal renal tissues, and GSK-3β expression became stronger along with the increasing of the grade of either inflammatory cell infiltration or interstitial fibrosis/tubular atrophy in renal allograft tissue.
  
  
  CONCLUSIONS
  There might be a positive correlation between either inflammatory cell infiltration or interstitial fibrosis/tubular atrophy and high GSK-3β expression in renal allograft tissue.
  The virtual slide(s) for this article can be found here:http://www.diagnosticpathology.diagnomx.eu/vs/9924478946162998.
authors.affiliation: Kidney Tranplantation and Dialysis Center of PLA NO.181 Hospital, Clinical College of Southern Medical University, Guilin 524001, Guangxi, PR China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Yan
authors.forename: Baoyao
authors.initials: B
authors.lastname: Wang
authors.forename: Weiguo
authors.initials: W
authors.lastname: Sui
authors.forename: Guimian
authors.initials: G
authors.lastname: Zou
authors.forename: Huaizhou
authors.initials: H
authors.lastname: Chen
authors.forename: Shenping
authors.initials: S
authors.lastname: Xie
authors.forename: Hequn
authors.initials: H
authors.lastname: Zou
mesh_terms: D000328:Adult; D001284:Atrophy / Q000473:pathology; D051799:Delayed Graft Function / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005355:Fibrosis / Q000473:pathology; D038362:Glycogen Synthase Kinase 3 / Q000096:biosynthesis*; D000071679:Glycogen Synthase Kinase 3 beta; D006801:Humans; D007150:Immunohistochemistry; D007249:Inflammation / Q000473:pathology; D016030:Kidney Transplantation / Q000009:adverse effects*; D008297:Male; D008875:Middle Aged; D014184:Transplantation, Homologous
chemical_list: C000605506:GSK3B protein, human; D000071679:Glycogen Synthase Kinase 3 beta; D038362:Glycogen Synthase Kinase 3