title: Electroporation-mediated genetic vaccination for antigen mapping: application to Plasmodium falciparum VAR2CSA protein.
abstract: Genetic vaccination, consisting in delivering a genetically engineered plasmid DNA by a non-viral vector or technique into a tissue, is currently of great interest. New delivery technique including DNA transfer by electroporation recently greatly improved the potency of this concept. Because it avoids the step of producing a recombinant protein, it is particularly of use in studying the immunogenic properties of large proteins. Here we describe the use of electroporation mediated DNA immunization to identify important protective epitopes from the large VAR2CSA protein from Plasmodium falciparum implicated in the pathology of placental malaria. Immunizing mice and rabbit with DNA plasmids encoding different fragments of VAR2CSA leads to high titer antisera. Moreover an N-terminal region of the protein was found to induce protective functional antibodies.
authors.affiliation: Université Paris Descartes, Sorbonne Paris Cité, Paris, France.
authors.forename: Bita
authors.initials: B
authors.lastname: Bordbar
authors.forename: Sédami
authors.initials: S
authors.lastname: Gnidehou
authors.forename: Nicaise Tuikue
authors.initials: NT
authors.lastname: Ndam
authors.forename: Justin
authors.initials: J
authors.lastname: Doritchamou
authors.forename: Azizath
authors.initials: A
authors.lastname: Moussiliou
authors.forename: Mickael
authors.initials: M
authors.lastname: Quiviger
authors.forename: Philippe
authors.initials: P
authors.lastname: Deloron
authors.forename: Daniel
authors.initials: D
authors.lastname: Scherman
authors.forename: Pascal
authors.initials: P
authors.lastname: Bigey
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood / Q000235:genetics / Q000276:immunology*; D000953:Antigens, Protozoan / Q000235:genetics / Q000276:immunology*; D018274:Electroporation; D005260:Female; D018014:Gene Transfer Techniques; D007114:Immunization; D017780:Malaria Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D016778:Malaria, Falciparum / Q000150:complications / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control*; D051379:Mice; D010920:Placenta / Q000187:drug effects / Q000276:immunology / Q000469:parasitology; D010957:Plasmids / Q000235:genetics / Q000276:immunology; D010963:Plasmodium falciparum / Q000187:drug effects / Q000276:immunology*; D011247:Pregnancy; D015597:Pregnancy Complications, Parasitic / Q000276:immunology / Q000517:prevention & control*; D011817:Rabbits; D019444:Vaccines, DNA
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D017780:Malaria Vaccines; C512601:VAR2CSA protein, Plasmodium falciparum; D019444:Vaccines, DNA