title: Genetic diversity of Chikungunya virus, India 2006-2010: evolutionary dynamics and serotype analyses.
abstract: The genetic diversity of Chikungunya virus (CHIKV) causing recurring outbreaks in India since 2006 was studied. The 2006 epidemic was caused by a virus strain of the East, Central and South African (ECSA) genotype with 226A in the E1 glycoprotein. The variant strain with E1-A226V mutation caused outbreaks since 2007 in the state of Kerala where Aedes albopictus is the abundant mosquito vector. Molecular epidemiology data since 2007 is scarce from other regions of the country. RT-PCR, sequencing and phylogenetic analyses of CHIKV isolates from the 2009 to 2010 epidemics in the States of Tamil Nadu and Andhra Pradesh placed them in a separate clade within the ECSA lineage. The isolates of the study had 226A in the E1 glycoprotein. The isolates had a novel E1-K211E mutation that was under significant positive selection. E1-211E is highly conserved in the Asian genotype of the virus circulated by Aedes aegypti. Unique mutations in E2 glycoprotein were identified. The two sub-lineages of ECSA genotype circulating in India parallel the abundance of Ae. albopictus and Ae. aegypti. Novel mutations in the envelope glycoproteins suggest adaptive evolution of the virus to local vector abundance. Cross neutralization of the virus isolates from recurring Indian epidemics indicated that no distinct serotypes had evolved. The study has provided insights into the origin, distribution and evolutionary adaptation of the virus to local vector abundance in the region that has reportedly, the highest incidence of CHIKV infection in the world.
authors.affiliation: Bharat Biotech International Ltd, Hyderabad, India. sumathy@bharatbiotech.com
authors.forename: K
authors.initials: K
authors.lastname: Sumathy
authors.forename: Krishna M
authors.initials: KM
authors.lastname: Ella
mesh_terms: D018354:Alphavirus Infections / Q000453:epidemiology* / Q000821:virology; D002646:Chikungunya virus / Q000145:classification* / Q000235:genetics*; D004196:Disease Outbreaks; D019143:Evolution, Molecular; D014644:Genetic Variation; D007194:India / Q000453:epidemiology; D009154:Mutation; D010802:Phylogeny; D012641:Selection, Genetic; D012703:Serotyping / Q000379:methods; D014759:Viral Envelope Proteins / Q000235:genetics
chemical_list: D014759:Viral Envelope Proteins