title: Fulminant cryptosporidiosis after near-drowning: a human Cryptosporidium parvum strain implicated in invasive gastrointestinal adenocarcinoma and cholangiocarcinoma in an experimental model.
abstract: In the present work, we report the characterization of a Cryptosporidium parvum strain isolated from a patient who nearly drowned in the Deule River (Lille, France) after being discharged from the hospital where he had undergone allogeneic stem cell transplantation. After being rescued and readmitted to the hospital, he developed fulminant cryptosporidiosis. The strain isolated from the patient's stools was identified as C. parvum II2A15G2R1 (subtype linked to zoonotic exposure) and inoculated into SCID mice. In this host, this virulent C. parvum isolate induced not only severe infection but also invasive gastrointestinal and biliary adenocarcinoma. The observation of adenocarcinomas that progressed through all layers of the digestive tract to the subserosa and spread via blood vessels confirmed the invasive nature of the neoplastic process. These results indicate for the first time that a human-derived C. parvum isolate is able to induce digestive cancer. This study is of special interest considering the exposure of a large number of humans and animals to this waterborne protozoan, which is highly tumorigenic when inoculated in a rodent model.
authors.affiliation: Centre d’Infection et d’Immunité de Lille (CIIL), Institut Pasteur de Lille, INSERM U1019, CNRS UMR 8402, Université Lille Nord de France, Lille, France. gabriela.certad@pasteur-lille.fr
authors.forename: Gabriela
authors.initials: G
authors.lastname: Certad
authors.forename: Sadia
authors.initials: S
authors.lastname: Benamrouz
authors.forename: Karine
authors.initials: K
authors.lastname: Guyot
authors.forename: Anthony
authors.initials: A
authors.lastname: Mouray
authors.forename: Thierry
authors.initials: T
authors.lastname: Chassat
authors.forename: Nicolas
authors.initials: N
authors.lastname: Flament
authors.forename: Laurence
authors.initials: L
authors.lastname: Delhaes
authors.forename: Valerie
authors.initials: V
authors.lastname: Coiteux
authors.forename: Baptiste
authors.initials: B
authors.lastname: Delaire
authors.forename: Marleen
authors.initials: M
authors.lastname: Praet
authors.forename: Claude
authors.initials: C
authors.lastname: Cuvelier
authors.forename: Pierre
authors.initials: P
authors.lastname: Gosset
authors.forename: Eduardo
authors.initials: E
authors.lastname: Dei-Cas
authors.forename: Colette
authors.initials: C
authors.lastname: Creusy
mesh_terms: D000230:Adenocarcinoma / Q000469:parasitology*; D000818:Animals; D018281:Cholangiocarcinoma / Q000469:parasitology*; D003457:Cryptosporidiosis / Q000175:diagnosis* / Q000473:pathology; D016785:Cryptosporidium parvum / Q000302:isolation & purification* / Q000472:pathogenicity*; D004195:Disease Models, Animal; D005243:Feces / Q000469:parasitology; D005602:France; D006801:Humans; D007414:Intestinal Neoplasms / Q000469:parasitology*; D051379:Mice; D016513:Mice, SCID; D015701:Near Drowning / Q000150:complications*