title: LogSpin: a simple, economical and fast method for RNA isolation from infected or healthy plants and other eukaryotic tissues.
abstract: BACKGROUND
  Rapid RNA extraction is commonly performed with commercial kits, which are very expensive and can involve toxic reagents. Most of these kits can be used with healthy plant tissues, but do not produce consistently high-quality RNA from necrotic fungus-infected tissues or fungal mycelium.
  
  
  RESULTS
  We report on the development of a rapid and relatively inexpensive method for total RNA extraction from plants and fungus-infected tissues, as well as from insects and fungi, based on guanidine hydrochloride buffer and common DNA extraction columns originally used for the extraction and purification of plasmids and cosmids.
  
  
  CONCLUSIONS
  The proposed method can be used reproducibly for RNA isolation from a variety of plant species. It can also be used with infected plant tissue and fungal mycelia, which are typically recalcitrant to standard nucleic acid extraction procedures.
authors.affiliation: Department of Plant Pathology and Microbiology, The Robert H, Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, P,O, Box 12, Rehovot 76100, Israel. levym@agri.huji.ac.il.
authors.forename: Hila
authors.initials: H
authors.lastname: Yaffe
authors.forename: Kobi
authors.initials: K
authors.lastname: Buxdorf
authors.forename: Illil
authors.initials: I
authors.lastname: Shapira
authors.forename: Shachaf
authors.initials: S
authors.lastname: Ein-Gedi
authors.forename: Michal
authors.initials: M
authors.lastname: Moyal-Ben Zvi
authors.forename: Eyal
authors.initials: E
authors.lastname: Fridman
authors.forename: Menachem
authors.initials: M
authors.lastname: Moshelion
authors.forename: Maggie
authors.initials: M
authors.lastname: Levy