title: Nucleotide sequence and chromosomal localization of the gene for pierisin-1, a DNA ADP-ribosylating protein, in the cabbage butterfly Pieris rapae.
abstract: Cabbage butterfly, Pieris rapae, contains a unique DNA ADP-ribosylating protein, pierisin-1, which transfers ADP-ribose moiety of NAD to guanine bases of DNA. Pierisin-like proteins are only distributed in subtribes Pierina, Aporiina and Appiadina of the family Pieridae. In this study, we obtained genomic clones carrying the pierisin-1 gene from adult samples of P. rapae by plaque hybridization. The pierisin-1 gene was found to consist of two exons, 0.1-kb exon 1 and 3.9-kb exon 2, and a 2.3-kb intron. In addition, we could demonstrate that the putative promoter in the about 3-kb upstream region from the transcription start site of the gene include a transcriptional activating motif involved in immune pathways and hormonal regulation. We also examined chromosomal localization of the pierisin-1 gene. Fluorescence in situ hybridization (FISH) analysis using Cy3-labeled pierisin-1 genomic clone demonstrated the localization of the gene near the kinetochore in chromosome 9. Thus, we confirmed that the pierisin-1 gene is located in the genome of P. rapae.
authors.affiliation: Division of Cancer Development System, Carcinogenesis Research Group, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan. mayamamo@ncc.go.jp
authors.forename: Masafumi
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Azusa
authors.initials: A
authors.lastname: Takahashi-Nakaguchi
authors.forename: Yuko
authors.initials: Y
authors.lastname: Matsushima-Hibiya
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Nakano
authors.forename: Yukari
authors.initials: Y
authors.lastname: Totsuka
authors.forename: Shigeo
authors.initials: S
authors.lastname: Imanishi
authors.forename: Jun
authors.initials: J
authors.lastname: Mitsuhashi
authors.forename: Masahiko
authors.initials: M
authors.lastname: Watanabe
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Nakagama
authors.forename: Takashi
authors.initials: T
authors.lastname: Sugimura
authors.forename: Keiji
authors.initials: K
authors.lastname: Wakabayashi
mesh_terms: D036002:ADP Ribose Transferases / Q000235:genetics* / Q000378:metabolism*; D000244:Adenosine Diphosphate / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D059006:Chromosomes, Insect / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism*; D005091:Exons / Q000235:genetics; D049750:Genome, Insect / Q000235:genetics; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism*; D007438:Introns / Q000235:genetics; D007915:Lepidoptera / Q000166:cytology / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data
chemical_list: D019476:Insect Proteins; C113557:pierisin protein, insect; D000244:Adenosine Diphosphate; D004247:DNA; D036002:ADP Ribose Transferases