title: Identification of the herboxidiene biosynthetic gene cluster in Streptomyces chromofuscus ATCC 49982.
abstract: The 53-kb biosynthetic gene cluster for the novel anticholesterol natural product herboxidiene was identified in Streptomyces chromofuscus ATCC 49982 by genome sequencing and gene inactivation. In addition to herboxidiene, a biosynthetic intermediate, 18-deoxy-herboxidiene, was also isolated from the fermentation broth of S. chromofuscus ATCC 49982 as a minor metabolite.
authors.affiliation: Department of Biological Engineering, Utah State University, Logan, Utah, USA.
authors.forename: Lei
authors.initials: L
authors.lastname: Shao
authors.forename: Jiachen
authors.initials: J
authors.lastname: Zi
authors.forename: Jia
authors.initials: J
authors.lastname: Zeng
authors.forename: Jixun
authors.initials: J
authors.lastname: Zhan
mesh_terms: D053898:Biosynthetic Pathways / Q000235:genetics*; D003470:Culture Media / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005233:Fatty Alcohols / Q000378:metabolism*; D055786:Gene Knockout Techniques; D008969:Molecular Sequence Data; D005810:Multigene Family; D011714:Pyrans / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism*
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial; D005233:Fatty Alcohols; D011714:Pyrans; C076087:herboxidiene