title: The use of a 'bleach-etch-seal' deproteinization technique on MIH affected enamel.
abstract: OBJECTIVE
  To ascertain whether deproteinization pretreatment of molar-incisor hypomineralization (MIH) enamel affects resin sealant infiltration.
  
  
  METHODS
  Thirty one extracted MIH teeth were divided into three sections and randomly allocated into the Control (etch and FS), Treatment 1 (5% NaOCl, etched and fissure sealed), and Treatment 2 (5% NaOCl and fissure sealed with no etch) groups. Two hundred seventy nine sealant tag/enamel grade observations were recorded by scanning electron microscopy.
  
  
  RESULTS
  Control and Treatment 1 were similar in their outcomes, and Treatment 2 was markedly different. There was no statistical evidence to suggest that there was any difference between Treatment 1 and the Control Treatment (95% CI, 0.52, 1.51; P = 0.6). There was a marked difference between Treatment 2 and the Control Treatment (95% CI, 0.07, 0.25; P < 0.001). All treatments also demonstrated a high-predicted probability of obtaining 'poor' sealant tags (Control = 47%, Treatment 1 = 49%, and Treatment 2 = 40%).
  
  
  CONCLUSIONS
  The findings suggest that there was no significant difference in the tag quality between the conventional technique (Control) and the 'bleach-etch-seal' technique (Treatment 1). There was no benefit in pre-treating with NaOCl alone (without etch) before sealing. This research also showed that there was a high-predicted probability of obtaining 'poor' sealant tags in MIH enamel, regardless of which of the three treatments was used.
authors.affiliation: Department of Child Dental Health, University of Bristol, Bristol Dental Hospital, Bristol, UK. gandhishan@hotmail.com
authors.forename: Shan
authors.initials: S
authors.lastname: Gandhi
authors.forename: Peter
authors.initials: P
authors.lastname: Crawford
authors.forename: Peter
authors.initials: P
authors.lastname: Shellis
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods*; D002648:Child; D003188:Composite Resins / Q000737:chemistry / Q000627:therapeutic use; D001840:Dental Bonding / Q000379:methods; D003743:Dental Enamel / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D003744:Dental Enamel Hypoplasia / Q000473:pathology / Q000628:therapy*; D003745:Dental Enamel Permeability / Q000187:drug effects*; D003763:Dental Leakage; D004359:Drug Therapy, Combination; D006801:Humans; D010895:Pit and Fissure Sealants / Q000737:chemistry / Q000627:therapeutic use*; D016037:Single-Blind Method; D012973:Sodium Hypochlorite / Q000008:administration & dosage; D013499:Surface Properties; D058205:Tooth Bleaching Agents / Q000008:administration & dosage*
chemical_list: D003188:Composite Resins; D010895:Pit and Fissure Sealants; D058205:Tooth Bleaching Agents; D012973:Sodium Hypochlorite