title: [Simultaneous determination of three sesquiterpene lactones in Inula hupehensis by RP-HPLC].
abstract: OBJECTIVE
  A RP-HPLC method was developed for simultaneous determination of bigelovin, ergolide and tomentosin in Inula hupehensis.
  
  
  METHODS
  An Agilent C18 column (4.6 mm x 250 mm, 5 microm) was used for separation at 40 degrees C. The mobile phase was acetonitrile-water, and the flow rate was 1.2 mL x min(-1). The detection wavelength was set at 210 nm.
  
  
  RESULTS
  The method has good linearity in the ranges of 0.01792-0.1792 g x L(-1) (r =0.9999) for bigelovin, 0.0424-0.4240 g x L(-1) (r =0.9996) for ergolide, and 0.044 8-0.4480 g x L(-1) (r = 0.9996) for tomentosin. The average recoveries of bigelovin, ergolide, and tomentosin were 98.5%, 98.2%, 98.4%, with the RSD of 1. 3%, 1.3%, 1.7%, respectively. The results demonstrated that there was a significant difference in the contents of three sequterpene lactones among the tested Inulae Flos.
  
  
  CONCLUSIONS
  The results indicated that the present RP-HPLC method is simple, quick and accurate, and can be used for the quality control of I. hupehensis, especially for the authentication of Inulae Flos.
authors.affiliation: Department of Drug Discovery, School of Pharmaceutical Science, Hebei Medical University, Shijiazhuang 050017, China. tianshanyoulan@163.com
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Wang
authors.forename: Xianghe
authors.initials: X
authors.lastname: Li
authors.forename: Yi
authors.initials: Y
authors.lastname: Guo
authors.forename: Jinlong
authors.initials: J
authors.lastname: Qi
authors.forename: Linfei
authors.initials: L
authors.lastname: Su
authors.forename: Yan
authors.initials: Y
authors.lastname: Fu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D038143:Inula / Q000737:chemistry* / Q000378:metabolism; D007783:Lactones / Q000032:analysis*; D012717:Sesquiterpenes / Q000032:analysis*
chemical_list: D007783:Lactones; D012717:Sesquiterpenes; C099797:ergolide; C454646:tomentosin; C099796:bigelovin