title: Label-free and substrate-free potentiometric aptasensing using polycation-sensitive membrane electrodes.
abstract: A potentiometric label-free and substrate-free (LFSF) aptasensing strategy which eliminates the labeling, separation, and immobilization steps is described in this paper. An aptamer binds specifically to a target molecule via reaction incubation, which could induce a change in the aptamer conformation from a random coil-like configuration to a rigid folded structure. Such a target binding-induced aptamer conformational change effectively prevents the aptamer from electrostatically interacting with the protamine binding domain. This could either shift the response curve for the potentiometric titration of the aptamer with protamine as monitored by a conventional polycation-sensitive membrane electrode or change the current-dependent potential detected by a protamine-conditioned polycation-sensitive electrode with the pulsed current-driven ion fluxes of protamine across the polymeric membrane. Using adenosine triphosphate (ATP) as a model analyte, the proposed concept offers potentiometric detection of ATP down to the submicromolar concentration range and has been applied to the determination of ATP in HeLa cells. In contrast to the current LFSF aptasensors based on optical detection, the proposed strategy allows the LFSF biosensing of aptamer/target binding events in a homogeneous solution via electrochemical transduction. It is anticipated that the proposed strategy will lay a foundation for development of potentiometric sensors for LFSF aptasensing of a variety of analytes where target binding-induced conformational changes such as the formation of folded structures and the opening of DNA hairpin loops are involved.
authors.affiliation: Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, Shandong 264003, P. R. China.
authors.forename: Jiawang
authors.initials: J
authors.lastname: Ding
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Xuewei
authors.initials: X
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Qin
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques; D004563:Electrochemistry; D004566:Electrodes; D006367:HeLa Cells; D006494:Heparin Antagonists / Q000378:metabolism; D006801:Humans; D008567:Membranes, Artificial; D011073:Polyamines / Q000737:chemistry*; D000071228:Polyelectrolytes; D011199:Potentiometry; D011479:Protamines / Q000378:metabolism
chemical_list: D000255:Adenosine Triphosphate; D052157:Aptamers, Nucleotide; D006494:Heparin Antagonists; D008567:Membranes, Artificial; D011073:Polyamines; D000071228:Polyelectrolytes; D011479:Protamines