title: [Study on effect of fushenkeli on expression of TAK1 in human renal tubular epithelial cells and its possible mechanism].
abstract: OBJECTIVE
  To investigate the effects of fushenkeli on the expression of TAK1 in the proliferation of the renal tubular epithelial cells induced by TGF-beta1 and its possible mechanism.
  
  
  METHODS
  Human renal tubular epithelial (HK-2) cells were divided into five groups:blank control group, TGF-beta1 group (5 microg x L(-1)), intervention group 1 (5 microg x L(-1) of TGF-beta1 + 100 mg x L(-1) of fushenkeli), intervention group 2 (5 microg x L(-1) of TGF-beta1 + 500 mg x L(-1) of fushenkeli) and intervention group 3 (5 microg x L(-1) of TGF-beta1 + 1 g x L(-1) of fushenkeli). HK-2 proliferation was detected by methyl thiazolyl tetrazolium (MTT) assay. Type IV collagen in the supernatants of the cultured HK-2 was detected by ELISA at 12, 24, 48 hours respectively. The protein and mRNA expressions of TAK1 was measured by Western blot and real-time quantitative PCR.
  
  
  RESULTS
  1) The cell proliferation and the expression of type IV collagen were increased compared with the control group (P<0.05, P<0.01), but they were decreased in intervention group. 2) The expressions of protein and mRNA of TAK1 in TGF-beta1 group were upregulating significantly compared with control group (P<0.01), but they were downregulating in intervention group, especially in intervention group 3.
  
  
  CONCLUSIONS
  Fushenkeli could inhibits TAK1 expression induced by TGF-beta1 in the proliferation of HK-2 cell.
authors.affiliation: Deparment of Nephrology, First Hospital, Lanzhou University, Lanzhou 730000, China. landaliuminglong@163.com
authors.forename: Minglong
authors.initials: M
authors.lastname: Liu
authors.forename: Yongxiang
authors.initials: Y
authors.lastname: Zeng
authors.forename: Shouyan
authors.initials: S
authors.lastname: Lu
authors.forename: Jianhua
authors.initials: J
authors.lastname: Li
authors.forename: Tianxi
authors.initials: T
authors.lastname: Liu
mesh_terms: D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D024141:Collagen Type IV / Q000187:drug effects / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007684:Kidney Tubules / Q000166:cytology*; D020930:MAP Kinase Kinase Kinases / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism*; D053773:Transforming Growth Factor beta1 / Q000008:administration & dosage; D000099151:MAP Kinase Kinase Kinase 7
chemical_list: D024141:Collagen Type IV; D004365:Drugs, Chinese Herbal; D020930:MAP Kinase Kinase Kinases; D012333:RNA, Messenger; D053773:Transforming Growth Factor beta1; D000099151:MAP Kinase Kinase Kinase 7