title: Enamel alterations in serotonin 2B receptor knockout mice.
abstract: The role of the serotonin 2B receptor (5-HT(2B) R) in enamel formation and mineralization was explored in adult 5HT(2B) R knockout (KO) mice compared with wild-type (WT) mice. In the molar, quantitative data obtained by micro-computed tomography imaging showed that the overall volume of the enamel layer was firmly reduced in KO mice. Defective mineralization was ascertained by energy-dispersive X-ray microanalysis. We also observed, using scanning electron microscopy, that parazones in the KO mice included two or three helicoidally twisted rods within Hunter-Schreger bands, instead of a single rod, as found in the WT mice. Minor disturbances were also detected in the incisors of KO mice. Structural modifications, thinner enamel crystallites, and porosities observed in KO mice indicate that the 5-HT(2B) R-mediated signaling pathways as part of the enamel formation process. These data provide a basis for evaluating the role of 5-HT(2B) R in ameloblast functions. Defects observed in the mineralization and structure of enamel in KO mice highlight that the 5-HT(2B) R interferes with the mechanisms directing amelogenesis.
authors.affiliation: INSERM, UMR-S 747, F-75006, Paris, France.
authors.forename: Yassine
authors.initials: Y
authors.lastname: Harichane
authors.forename: Sasha
authors.initials: S
authors.lastname: Dimitrova-Nakov
authors.forename: Arnaud
authors.initials: A
authors.lastname: Marchadier
authors.forename: Corinne
authors.initials: C
authors.lastname: Collet
authors.forename: Anne
authors.initials: A
authors.lastname: Baudry
authors.forename: Catherine
authors.initials: C
authors.lastname: Vidal
authors.forename: Agnès
authors.initials: A
authors.lastname: Kamoun-Goldrat
authors.forename: Odile
authors.initials: O
authors.lastname: Kellermann
authors.forename: Michel
authors.initials: M
authors.lastname: Goldberg
mesh_terms: D000566:Amelogenesis / Q000235:genetics*; D000818:Animals; D003743:Dental Enamel / Q000002:abnormalities* / Q000000981:diagnostic imaging / Q000648:ultrastructure; D005260:Female; D007180:Incisor / Q000473:pathology; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D008963:Molar / Q000473:pathology; D044403:Receptor, Serotonin, 5-HT2B / Q000235:genetics* / Q000502:physiology*; D014074:Tooth Calcification / Q000235:genetics; D055114:X-Ray Microtomography
chemical_list: D044403:Receptor, Serotonin, 5-HT2B