title: S-adenosylmethionine does not reduce ischaemia-reperfusion injury to a marginal liver graft in an in vivo experiment.
abstract: OBJECTIVE
  There are a limited number of appropriate cadaver liver donors. One possible solution is the use of marginal liver grafts from cadaver donors for liver transplantation.
  
  
  METHODS
  Rats with liver steatosis were divided into four containing seven animals each: I-a: steatotic liver grafts; +S-adenosylmethionine; I-b: steatotic liver grafts were transplanted; no S-adenosylmethionine; II-a: normal liver grafts, +S-adenosylmethionine; II-b: normal liver grafts. Blood samples were taken at days -1, 3 and 14.
  
  
  RESULTS
  ALT values at day 14: 1.75 ± 1.10µkat/L (in group I-a), 1.91 ± 1.41µkat/L (in group I-b), 2.13±1.85µkat/L (in group II-a) and 2.08 ± 1.35µkat/L (in group II-b). There were no significant differences between these values. GSH values at day 14 post-transplantation were: 44.90 ± 8.61µM/mg (in group I-a), 43.82±8.58µM/mg (in group I-b), 41.65 ± 4.87µM/mg (in group II-a) and 42.71 ± 4.17µM/mg (in control group II-b).
  
  
  CONCLUSIONS
  Our study did not demonstrate the positive effect of S-adenosylmethionine on ischaemia-reperfusion injury during liver transplantation in rats.
authors.affiliation: Department of Surgery, Central Military Hospital, Prague, Czech Republic. tomas@pantoflicek.cz
authors.forename: Tomas
authors.initials: T
authors.lastname: Pantoflicek
authors.forename: Eva
authors.initials: E
authors.lastname: Koblihova
authors.forename: Miroslav
authors.initials: M
authors.lastname: Ryska
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D015415:Biomarkers / Q000097:blood; D002796:Choline Deficiency / Q000150:complications; D004195:Disease Models, Animal; D005234:Fatty Liver / Q000150:complications* / Q000209:etiology / Q000473:pathology; D005978:Glutathione / Q000097:blood; D016031:Liver Transplantation / Q000009:adverse effects*; D008715:Methionine / Q000172:deficiency; D051381:Rats; D011917:Rats, Inbred Lew; D015427:Reperfusion Injury / Q000097:blood / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D012436:S-Adenosylmethionine / Q000494:pharmacology*; D012720:Severity of Illness Index; D013997:Time Factors
chemical_list: D015415:Biomarkers; D012436:S-Adenosylmethionine; D008715:Methionine; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D005978:Glutathione