title: Bioconversion of ethyl (R)-4-cyano-3-hydroxybutyate into (R)-ethyl-3-hydroxyglutarate via an indirect pathway by Rhodococcus boritolerans.
abstract: (R)-Ethyl-3-hydroxyglutarate, (R)-3, is an intermediate in the synthesis of the statin side chain. Here, a new two-step, indirect biotransformation pathway involving the formation of ethyl (R)-4-carbamoyl-3-hydroxybutanoate, (R)-2, as an intermediate for (R)-3 production was developed using Rhodococcus boritolerans with ethyl (R)-4-cyano-3-hydroxybutyate, (R)-1, as substrate. Maximum conversion was with 10 g (R)-1/l, 7 g cells/l (dry wt), pH 7.5 and 25°C. A yield of 98 ± 0.5% (w/w) was attained within 8 h.
authors.affiliation: Department of Life Science, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
authors.forename: Ming-Jia
authors.initials: MJ
authors.lastname: Yang
authors.forename: Xiang-Jing
authors.initials: XJ
authors.lastname: Wang
authors.forename: Zhong-Yi
authors.initials: ZY
authors.lastname: Yang
authors.forename: Jing
authors.initials: J
authors.lastname: An
authors.forename: Wen-Sheng
authors.initials: WS
authors.lastname: Xiang
authors.forename: Ji
authors.initials: J
authors.lastname: Zhang
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000378:metabolism*; D001711:Biotransformation; D003470:Culture Media / Q000737:chemistry; D005977:Glutarates / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D012240:Rhodococcus / Q000235:genetics* / Q000378:metabolism*; D013696:Temperature
chemical_list: C108959:3-hydroxyglutaric acid; D003470:Culture Media; D005977:Glutarates; D020155:3-Hydroxybutyric Acid