title: Probing inhibitory effects of nanocrystalline cellulose: inhibition versus surface charge.
abstract: NCC derived from different biomass sources was probed for its plausible cytotoxicity by electric cell-substrate impedance sensing (ECIS). Two different cell lines, Spodoptera frugiperda Sf9 insect cells and Chinese hamster lung fibroblast V79, were exposed to NCC and their spreading and viability were monitored and quantified by ECIS. Based on the 50%-inhibition concentration (ECIS(50)), none of the NCC produced was judged to have any significant cytotoxicity on these two cell lines. However, NCC derived from flax exhibited the most pronounced inhibition on Sf9 compared to hemp and cellulose powder. NCCs from flax and hemp pre-treated with pectate lyase were also less inhibitory than NCCs prepared from untreated flax and hemp. Results also suggested a correlation between the inhibitory effect and the carboxylic acid contents on the NCC.
authors.affiliation: Biotechnology Research Institute, National Research Council Canada, 6100 Royalmount Avenue, Montreal, Quebec H4P 2R2, Canada.
authors.forename: Keith B
authors.initials: KB
authors.lastname: Male
authors.forename: Alfred C W
authors.initials: AC
authors.lastname: Leung
authors.forename: Johnny
authors.initials: J
authors.lastname: Montes
authors.forename: Amine
authors.initials: A
authors.lastname: Kamen
authors.forename: John H T
authors.initials: JH
authors.lastname: Luong
mesh_terms: D000818:Animals; D002460:Cell Line; D002482:Cellulose / Q000737:chemistry*; D006224:Cricetinae; D003412:Cricetulus; D004566:Electrodes; D005347:Fibroblasts / Q000187:drug effects; D053758:Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D018411:Spodoptera / Q000166:cytology / Q000187:drug effects
chemical_list: D002482:Cellulose