title: Analysis of assembly of secreted mucins.
abstract: Studies of assembly and secretion of gel-forming mucins are complex. The pulse-chase methods for mucins described here include metabolic radiolabeling and labeling in animals with azido-GalNAc. The labeled mucins are analyzed by composite agarose-polyacrylamide gel electrophoresis and autoradiography or by mucus-preserving tissue fixation and Click-iT(®) chemistry.
authors.affiliation: Department of Medical Biochemistry, Mucin Biology Group, University of Gothenburg, Gothenburg, Sweden.
authors.forename: Malin E V
authors.initials: ME
authors.lastname: Johansson
authors.forename: Gunnar C
authors.initials: GC
authors.lastname: Hansson
mesh_terms: D000818:Animals; D001345:Autoradiography; D001386:Azides / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D006031:Glycosylation; D051379:Mice; D009077:Mucins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D017350:N-Acetylgalactosaminyltransferases / Q000737:chemistry / Q000378:metabolism; D016707:Tissue Fixation
chemical_list: D001386:Azides; D009077:Mucins; D017350:N-Acetylgalactosaminyltransferases