title: Pilot-scale production and viability analysis of freeze-dried probiotic bacteria using different protective agents.
abstract: The functional food industry requires an improvement of probiotic strain stability during storage, especially when they are stored at room temperature. In this study, the viability of freeze-dried Lactobacillus rhamnosus IMC 501(®) and Lactobacillus paracasei IMC 502(®) using different protective agents (i.e., glycerine, mannitol, sorbitol, inulin, dextrin, Crystalean(®)) was determined and compared with semi skimmed milk (SSM) control. No significant differences were observed between the tested protectants and the control (SSM) during storage at refrigerated conditions. During storage at room temperature, only glycerine was found to stabilize viability better than other tested substances.
authors.affiliation: Synbiotec Srl, Camerino, Italy. michele.savini@studenti.unicam.it
authors.forename: Michele
authors.initials: M
authors.lastname: Savini
authors.forename: Cinzia
authors.initials: C
authors.lastname: Cecchini
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Verdenelli
authors.forename: Stefania
authors.initials: S
authors.lastname: Silvi
authors.forename: Carla
authors.initials: C
authors.lastname: Orpianesi
authors.forename: Alberto
authors.initials: A
authors.lastname: Cresci
mesh_terms: D015169:Colony Count, Microbial; D003451:Cryoprotective Agents / Q000494:pharmacology*; D061353:Food Storage; D005612:Freeze Drying; D006801:Humans; D007778:Lactobacillus / Q000254:growth & development*; D050296:Microbial Viability; D010865:Pilot Projects; D019936:Probiotics; D013696:Temperature
chemical_list: D003451:Cryoprotective Agents