title: Isovolaemic hemodilution with gelatin and hydroxyethylstarch 130/0.42: effects on hemostasis in piglets.
abstract: OBJECTIVE
  Artificial colloids, frequently used to prevent hemorrhagic shock in children, impair blood coagulation. To determine the impact of acute isovolaemic hemodilution with artificial colloids on clot formation, we conducted an experimental study in a pediatric animal model.
  
  
  METHODS
  Fifteen piglets underwent hemorrhage by withdrawing 40 ml·kg(-1) of blood volume in steps of 10 ml·kg(-1) each within 1 hour. After each withdrawal, the blood loss was randomly compensated by administering 4% gelatin (GEL) or hydroxylethyl starch 130/0.42 (HES) in a ratio of 1 : 1, or isotonic crystalloid solution (ICS) in a ratio of 1 : 4 for isovolaemic hemodilution. Quality of clot formation and platelet function was measured using Thrombelastometry (ROTEM(®)) and Multiple electrode impedance aggregometry (Multiplate(®)) after 10, 20, and 40 ml·kg(-1) blood replacement.
  
  
  RESULTS
  Moderate hemodilution (10-20 ml·kg(-1) blood replacement) caused no significant differences among groups (e.g. INTEM(®)-MCF after 20 ml·kg(-1) blood replacement (ICS vs GEL vs HES, P > 0.05). Profound hemodilution with 40 ml·kg(-1) blood replacement showed a significant difference between ICS and both colloids (P < 0.05), but no significant differences between GEL and HES.
  
  
  CONCLUSIONS
  Impairment of clot formation by moderate isovolaemic hemodilution did not significantly differ between ICS, GEL, and HES. Profound hemodilution of more than 50% of the estimated blood volume with GEL and HES caused significant impairment of clot formation in comparison to ICS and has to be considered when using high amounts of these synthetic colloids.
authors.affiliation: Clinic of Anaesthesiology, Hannover Medical School, Hannover, Germany. witt.lars-henrik@mh-hannover.de
authors.forename: Lars
authors.initials: L
authors.lastname: Witt
authors.forename: Wilhelm Alexander
authors.initials: WA
authors.lastname: Osthaus
authors.forename: Wiebke
authors.initials: W
authors.lastname: Jahn
authors.forename: Niels
authors.initials: N
authors.lastname: Rahe-Meyer
authors.forename: Alexander
authors.initials: A
authors.lastname: Hanke
authors.forename: Florian
authors.initials: F
authors.lastname: Schmidt
authors.forename: Martin
authors.initials: M
authors.lastname: Boehne
authors.forename: Robert
authors.initials: R
authors.lastname: Sümpelmann
mesh_terms: D000136:Acid-Base Equilibrium; D000465:Algorithms; D000818:Animals; D001777:Blood Coagulation; D001786:Blood Glucose / Q000378:metabolism; D001802:Blood Substitutes; D001810:Blood Volume / Q000502:physiology; D001835:Body Weight / Q000502:physiology; D002302:Cardiac Output; D017097:Electric Impedance; D005340:Fibrinogen / Q000032:analysis; D005780:Gelatin; D006400:Hematocrit; D006438:Hemodilution / Q000379:methods*; D006439:Hemodynamics / Q000187:drug effects; D006454:Hemoglobins / Q000378:metabolism; D006470:Hemorrhage / Q000628:therapy; D006487:Hemostasis / Q000187:drug effects*; D006895:Hydroxyethyl Starch Derivatives; D010974:Platelet Aggregation; D013552:Swine; D013916:Thrombelastography; D014914:Whole Blood Coagulation Time
chemical_list: D001786:Blood Glucose; D001802:Blood Substitutes; D006454:Hemoglobins; D006895:Hydroxyethyl Starch Derivatives; D005780:Gelatin; D005340:Fibrinogen