title: Determination of hyperoside and isoquercitrin in rat plasma by membrane-protected micro-solid-phase extraction with high-performance liquid chromatography.
abstract: A novel method, micro-solid-phase extraction based on membrane-protected molecularly imprinted polymer, was developed to extract hyperoside and isoquercitrin in rat plasma. Synthesized hyperoside MIPs were packed in a porous polyether sulfone membrane envelope to perform extraction. The parameters sorbent materials, membrane types, extraction time and desorption conditions were optimized for micro-solid-phase extraction. Under the optimal conditions, correlation coefficients, 0.998 and 0.999, were obtained for hyperoside and isoquercitrin, respectively, with the linear range between 1 and 120 μg/mL. The absolute extraction recoveries from 84.5 to 89.3% were found. The method detection limits of hyperoside and isoquercitrin were 0.24 and 0.22 μg/mL, respectively. Compared with traditional methods, solid-phase extraction, liquid-liquid extraction and protein precipitation, the developed method was simple, highly efficient for extraction, environmentally friendly, and particularly suitable for complex biological samples.
authors.affiliation: College of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang, PR China.
authors.forename: Xiao-Ying
authors.initials: XY
authors.lastname: Yin
authors.forename: Yong-Ming
authors.initials: YM
authors.lastname: Luo
authors.forename: Jian-Jiang
authors.initials: JJ
authors.lastname: Fu
authors.forename: You-Quan
authors.initials: YQ
authors.lastname: Zhong
authors.forename: Qing-Shan
authors.initials: QS
authors.lastname: Liu
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D011794:Quercetin / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry; D051381:Rats; D052616:Solid Phase Extraction
chemical_list: C016527:isoquercitrin; C021304:hyperoside; D011794:Quercetin