title: Use of intercellular washing fluid to investigate the secreted proteome of the rice-Magnaporthe interaction.
abstract: Early interactions between invading penetration hyphae of the pathogenic fungus Magnaporthe oryzae and rice cells occur at the apoplast, the free diffusional space outside the plasma membrane of leaves. After initial colonization, intercellular hyphae are again in intimate contact with the rice apoplast. While several studies have looked at proteomics in rice-Magnaporthe interactions, none have focused on apoplast localized proteins. We adjusted a protocol for intercellular washing fluids (IWF) to rice leaves infected with Magnaporthe oryzae for proteomic analysis. In our IWF extract, we identified several proteins associated with compatible or incompatible pathogen interactions. Three DUF26 domain proteins were identified as changing in abundance 12 h after inoculation, confirming DUF26 domain-containing proteins are among early, pathogen stress-responsive proteins induced by infection with Magnaporthe oryzae. A Magnaporthe cyclophilin, previously identified as a virulence factor was also identified in the intercellular washing fluid.
authors.affiliation: Iwate Biotechnology Research Center, 22-174-4 Narita, Kitakami, Iwate 024-0003, Japan.
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Shenton
authors.forename: Thomas
authors.initials: T
authors.lastname: Berberich
authors.forename: Masaharu
authors.initials: M
authors.lastname: Kamo
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Yamashita
authors.forename: Hideharu
authors.initials: H
authors.lastname: Taira
authors.forename: Ryohei
authors.initials: R
authors.lastname: Terauchi
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional; D045604:Extracellular Fluid / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism*; D054884:Host-Pathogen Interactions; D020082:Magnaporthe / Q000502:physiology*; D013058:Mass Spectrometry; D012275:Oryza / Q000378:metabolism* / Q000382:microbiology / Q000502:physiology; D010935:Plant Diseases / Q000382:microbiology*; D018515:Plant Leaves / Q000378:metabolism / Q000382:microbiology; D010940:Plant Proteins / Q000378:metabolism*; D020543:Proteome; D040901:Proteomics
chemical_list: D005656:Fungal Proteins; D010940:Plant Proteins; D020543:Proteome