title: Association of medication with the human plasma N-glycome.
abstract: Glycosylation is highly variable depending on many environmental factors. Using our fully quantitative high-throughput normal phase hydrophilic interaction liquid chromatography platform we have identified glycosylation changes associated with medication in the plasma N-glycome from three different population cohorts: ORCADES from the Orkney Islands in Scotland and CROATIA-Vis and CROATIA-Korcula from the Croatian islands of Vis and Korcula. Associations between glycosylation and the use of hormones (oral contraceptives, hormone replacement therapy), nonsteroidal anti-inflammatory drugs (aspirin and other NSAIDs), oral steroids (prednisolone) and steroid inhalers (beclomethasone) were investigated. Significant differences associated with usage of oral contraceptives were found with increased core-fucosylated biantennary glycans. Decreases in core-fucosylated biantennary glycans, core-fucosylated triantennary glycans with outer-arm fucose, and high mannosylated glycans were associated with the use of anti-inflammatory drugs. All of the changes in glycosylation were independent of blood group status. In conclusion, hormones and anti-inflammatory medication were associated with changes in glycosylation, possibly as a result of the modulatory effect of these drugs on the inflammatory response. In general, cancer is associated with inflammation, and many glycoproteins in the plasma are acute phase related to the host response. These preliminary data indicate the importance of correcting the levels of glycans used as biomarkers for the effects of medication.
authors.affiliation: NIBRT Dublin-Oxford Glycobiology Laboratory, National Institute for Bioprocessing Research and Training , Fosters Avenue, Mount Merrion, Blackrock, Dublin 4, Ireland.
authors.forename: Radka
authors.initials: R
authors.lastname: Saldova
authors.forename: Jennifer E
authors.initials: JE
authors.lastname: Huffman
authors.forename: Barbara
authors.initials: B
authors.lastname: Adamczyk
authors.forename: Ana
authors.initials: A
authors.lastname: Mužinić
authors.forename: Jayesh J
authors.initials: JJ
authors.lastname: Kattla
authors.forename: Maja
authors.initials: M
authors.lastname: Pučić
authors.forename: Mislav
authors.initials: M
authors.lastname: Novokmet
authors.forename: Jodie L
authors.initials: JL
authors.lastname: Abrahams
authors.forename: Caroline
authors.initials: C
authors.lastname: Hayward
authors.forename: Igor
authors.initials: I
authors.lastname: Rudan
authors.forename: Sarah H
authors.initials: SH
authors.lastname: Wild
authors.forename: Alan F
authors.initials: AF
authors.lastname: Wright
authors.forename: Ozren
authors.initials: O
authors.lastname: Polašek
authors.forename: Gordan
authors.initials: G
authors.lastname: Lauc
authors.forename: Harry
authors.initials: H
authors.lastname: Campbell
authors.forename: James F
authors.initials: JF
authors.lastname: Wilson
authors.forename: Pauline M
authors.initials: PM
authors.lastname: Rudd
mesh_terms: D000017:ABO Blood-Group System / Q000097:blood* / Q000737:chemistry; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D005260:Female; D054794:Glycomics; D006031:Glycosylation / Q000187:drug effects; D006728:Hormones / Q000494:pharmacology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011134:Polysaccharides / Q000097:blood* / Q000737:chemistry; D020543:Proteome / Q000378:metabolism*; D013256:Steroids / Q000494:pharmacology*; D055815:Young Adult
chemical_list: D000017:ABO Blood-Group System; D000894:Anti-Inflammatory Agents, Non-Steroidal; D015415:Biomarkers; D006728:Hormones; D011134:Polysaccharides; D020543:Proteome; D013256:Steroids