title: [Identification of marsdeniae tenacissimae caulis and its adulterants by RAPD].
abstract: OBJECTIVE
  To identify Marsdeniae Tenacissimae Caulis and its adulterants by RAPD (random amplified polymorphic DNA) and analyze the gene homology of Marsdeniae Tenacissimae Caulis from various habitats.
  
  
  METHODS
  General DNA was isolated from Marsdeniae tenacissimae Caulis which were from seven various habitats and its six adulterants by CTAB, the twenty RAPD was used to identify them.
  
  
  RESULTS
  Random primer 285 (GGG AAC CCT T) could amplify the gene of Marsdeniae tenacissimae Caulis from various habitats stablely, Marsdeniae tenacissimae Caulis and its adulterants could be identified by primer E01 (CCC AAG GTC C) effectively.
  
  
  CONCLUSIONS
  The method of RAPD can be used to identify Marsdeniae Tenacissimae Caulis and its adulterants, the gene of Marsdeniae tenacissimae Caulis from various habitats have homology.
authors.affiliation: Liaoning Universitiy of Chinese Traditional Medicine, Dalian 116600, China. syyycs@163.com
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Zhi-Dong
authors.initials: ZD
authors.lastname: Pei
authors.forename: Chong
authors.initials: C
authors.lastname: Ni
authors.forename: Ting-Guo
authors.initials: TG
authors.lastname: Kang
mesh_terms: D029062:Apocynaceae / Q000145:classification / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics*; D004340:Drug Contamination; D017343:Genes, Plant; D005819:Genetic Markers; D010802:Phylogeny; D018547:Plant Stems / Q000235:genetics; D010946:Plants, Medicinal / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D011786:Quality Control; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers