title: Study of antiproliferative effects of synthetic substances against lens epithelial cell line (SRA 01/04).
abstract: A cataract is a clouded area of the eye, which impairs vision. Cataracts can be caused by a natural hardening of the lens in the elderly, or may be the result of eye injury. However there is a treatment by extracapsular surgery, almost 50% of operations are followed by another posterior capsule opacification. This secondary cataract is due to abnormal cellular proliferation. Pharmacologic inhibition of this cellular proliferation would be a very promising treatment. The objective of our study is to test some antiproliferative drugs, less toxic than those currently used such as 5-FU or mytomycin C. We have investigated the in vitro effects of several molecules (V0 and its derivatives) on a proliferative human lens epithelial cell line (SRA 01/04). During a first step, we have measured the IC50 of each molecule. After this first screening, we have studied the kinetic of the cell growth with or without the molecules at different concentration. Then, flow cytometry was used to determine the phase of the cell cycle at which the proliferation stopped. This study has shown that 3 molecules V19, V1, and A190 have an interesting profile in vitro and were selected to analyze their mechanism of action.
authors.affiliation: Université de Nantes, Nantes Atlantique Université, IICIMED/ERT-A0902, Cancer du Poumon et Cibles Moléculaires (CPCM), UFR des Sciences Pharmaceutiques, Nantes, France.
authors.forename: Marine
authors.initials: M
authors.lastname: Malleter
authors.forename: Catherine
authors.initials: C
authors.lastname: Jacquot
authors.forename: Benedicte
authors.initials: B
authors.lastname: Rousseau
authors.forename: Christophe
authors.initials: C
authors.lastname: Tomasoni
authors.forename: Didier
authors.initials: D
authors.lastname: Ducourneau
authors.forename: Philippe
authors.initials: P
authors.lastname: Tourette
authors.forename: Alain
authors.initials: A
authors.lastname: Pineau
authors.forename: Christos
authors.initials: C
authors.lastname: Roussakis
mesh_terms: D002386:Cataract / Q000188:drug therapy*; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D004847:Epithelial Cells / Q000187:drug effects; D006801:Humans; D007908:Lens, Crystalline / Q000166:cytology / Q000187:drug effects*; D004364:Pharmaceutical Preparations / Q000008:administration & dosage*
chemical_list: D004364:Pharmaceutical Preparations