title: Rapid accumulation of polymorphonuclear neutrophils in the Corpus luteum during prostaglandin F(2α)-induced luteolysis in the cow.
abstract: Prostaglandin F(2α) (PGF(2α)) induces luteolysis within a few days in cows, and immune cells increase in number in the regressing corpus luteum (CL), implying that luteolysis is an inflammatory-like immune response. We investigated the rapid change in polymorphonuclear neutrophil (PMN) numbers in response to PGF(2α) administration as the first cells recruited to inflammatory sites, together with mRNA of interleukin-8 (IL-8: neutrophil chemoattractant) and P-selectin (leukocyte adhesion molecule) in the bovine CL. CLs were collected by ovariectomy at various times after PGF(2α) injection. The number of PMNs was increased at 5 min after PGF(2α) administration, whereas IL-8 and P-selectin mRNA increased at 30 min and 2 h, respectively. PGF(2α) directly stimulated P-selectin protein expression at 5-30 min in luteal endothelial cells (LECs). Moreover, PGF(2α) enhanced PMN adhesion to LECs, and this enhancement by PGF(2α) was inhibited by anti-P-selectin antibody, suggesting that P-selectin expression by PGF(2α) is crucial in PMN migration. In conclusion, PGF(2α) rapidly induces the accumulation of PMNs into the bovine CL at 5 min and enhances PMN adhesion via P-selectin expression in LECs. It is suggested that luteolytic cascade by PGF(2α) may involve an acute inflammatory-like response due to rapidly infiltrated PMNs.
authors.affiliation: Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan.
authors.forename: Koumei
authors.initials: K
authors.lastname: Shirasuna
authors.forename: Sineenard
authors.initials: S
authors.lastname: Jiemtaweeboon
authors.forename: Sybille
authors.initials: S
authors.lastname: Raddatz
authors.forename: Akane
authors.initials: A
authors.lastname: Nitta
authors.forename: Hans-Joachim
authors.initials: HJ
authors.lastname: Schuberth
authors.forename: Heinrich
authors.initials: H
authors.lastname: Bollwein
authors.forename: Takashi
authors.initials: T
authors.lastname: Shimizu
authors.forename: Akio
authors.initials: A
authors.lastname: Miyamoto
mesh_terms: D000818:Animals; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D002452:Cell Count; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D003338:Corpus Luteum / Q000276:immunology / Q000502:physiology*; D015237:Dinoprost / Q000494:pharmacology*; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D016209:Interleukin-8 / Q000235:genetics; D003341:Luteolysis / Q000187:drug effects* / Q000276:immunology; D009504:Neutrophils / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D019007:P-Selectin / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013997:Time Factors
chemical_list: D016209:Interleukin-8; D019007:P-Selectin; D012333:RNA, Messenger; D015237:Dinoprost