title: Origin of Dryopteris shibipedis (Dryopteridaceae), a fern species extinct in the wild.
abstract: Dryopteris shibipedis was once treated as an extinct species in the Red List (2007) by the Japanese Ministry of the Environment, but 'rediscovered' in the Tsukuba Botanical Garden. To clarify its origin, using 'overlooked' cultivated stocks we analyzed nuclear PgiC intron sequences. As the PgiC genotype of D. shibipedis can be explained by a combination of alleles of D. kinkiensis and those of D. pacifica, the hypothesis of hybrid origin of the species is supported.
authors.affiliation: Department of Botany, National Museum of Nature and Science, 4-1-1 Amakubo, Tsukuba 305-0005, Japan. ebihara@kahaku.go.jp
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ebihara
authors.forename: Sadamu
authors.initials: S
authors.lastname: Matsumoto
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kato
mesh_terms: D002467:Cell Nucleus / Q000235:genetics*; D018744:DNA, Plant; D032492:Dryopteris / Q000145:classification* / Q000235:genetics*; D019143:Evolution, Molecular; D053476:Extinction, Biological; D014644:Genetic Variation; D005843:Geography; D006824:Hybridization, Genetic; D007438:Introns / Q000235:genetics*; D007564:Japan; D017422:Sequence Analysis, DNA
chemical_list: D018744:DNA, Plant