title: Effect of different fertilizers on nitrogen isotope composition and nitrate content of Brassica campestris.
abstract: The effect of different fertilizers on the δ(15)N value, nitrate concentration, and nitrate reductase activity of Brassica campestris and the δ(15)N value of soil has been investigated through a pot experiment. The δ(15)N mean value of B. campestris at the seedling stage observed in the composted chicken treatment (+8.65‰) was higher than that of chemical fertilizer treatment (+5.73‰), compost-chemical fertilizer (+7.53‰), and control check treatment (+7.86‰). There were significantly different δ(15)N values (p < 0.05) between B. campestris cultivated with composted chicken manure treatment and with chemical fertilizer treatment. The similar results were also found at the middle stage and the terminal stage. The variation of δ(15)N value in soil for different treatments was smaller than that of B. campestris, which was +6.71-+8.12‰, +6.83-+8.24‰, and +6.85-8.4‰, respectively, at seedling stage, middle stage, and terminal stage. With the growth of B. campestris, the nitrate content decreased in all treatments, and the nitrate reductase activity in B. campestris increased except for the CK. Results suggested that the δ(15)N values of B. campestris and soil were more effected by the fertilizer than by the dose level, and the δ(15)N value analysis could be used as a tool to discriminate the B. campestris cultivated with composted manure or chemical fertilizer.
authors.affiliation: Institute of Quality and Standards for Agricultural Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China. ywytea@163.com
authors.forename: Yuwei
authors.initials: Y
authors.lastname: Yuan
authors.forename: Ming
authors.initials: M
authors.lastname: Zhao
authors.forename: Zhiheng
authors.initials: Z
authors.lastname: Zhang
authors.forename: Tianjin
authors.initials: T
authors.lastname: Chen
authors.forename: Guiling
authors.initials: G
authors.lastname: Yang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
mesh_terms: D000818:Animals; D001937:Brassica / Q000737:chemistry* / Q000254:growth & development; D002645:Chickens; D005308:Fertilizers; D008372:Manure; D050901:Nitrate Reductase / Q000378:metabolism; D009566:Nitrates / Q000032:analysis*; D009587:Nitrogen Isotopes / Q000032:analysis*; D036226:Seedlings / Q000737:chemistry; D012987:Soil / Q000032:analysis
chemical_list: D005308:Fertilizers; D008372:Manure; D009566:Nitrates; D009587:Nitrogen Isotopes; D012987:Soil; D050901:Nitrate Reductase