title: Femtosecond laser nanostructuring for femtosensitive DNA detection.
abstract: In this paper, a new concept to achieve improved probe-target recognition has been devised by introducing a novel class of DNA-functionalized three-dimensional (3D), stand-free, and nanostructured electrodes. The gold nanofibrous electrodes were created using MHZ ultrafast laser material processing in air at ambient conditions. The developed nanofibrous DNA biosensor was characterized by cyclic voltammetry with the use of ferrocyanide as an electrochemical redox indicator. Currently, electrochemical signal enhancement which is of great significance for improving the sensitivity in DNA detection remains a great challenge. Through, enhanced surface area-to-volume ratio and more efficient arrangement of probe molecules on nanofibrous electrodes, our newly developed electrode system overcomes some of the sensitivity challenges of the existing systems. This nanofiber-based system realizes femtomolar (fM) sensitivity toward complementary target DNA, and demonstrates a very wide dynamic range (from 1 fM to 1 nM).
authors.affiliation: Department of Mechanical Engineering, Ryerson University, 350 Victoria Street, Toronto, Ontario, Canada M3N 2H8.
authors.forename: M
authors.initials: M
authors.lastname: Alubaidy
authors.forename: L
authors.initials: L
authors.lastname: Soleymani
authors.forename: K
authors.initials: K
authors.lastname: Venkatakrishnan
authors.forename: B
authors.initials: B
authors.lastname: Tan
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000032:analysis*; D055664:Electrochemical Techniques; D004566:Electrodes; D007834:Lasers; D049329:Nanostructures; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D004247:DNA