title: [Molecular hyperspectral imaging (MHSI) system and application in biochemical medicine].
abstract: A novel molecular hyperspectral imaging (MHSI) system based on AOTF (acousto-optic tunable filters) was presented. The system consists of microscope, AOTF-based spectrometer, matrix CCD, image collection card and computer. The spectral range of the MHSI is from 550 to 1 000 nm. The spectral resolution is less than 2 nm, and the spatial resolution is about 0.3 microm. This paper has also presented that spectral curves extracted from the corrected hyperspectral data of the sample, which have been preprocessed by the gray correction coefficient, can more truly represent biochemical characteristic of the sample. The system can supply not only single band images in the visible range, but also spectrum curve of random pixel of sample image. This system can be widely used in various fields of biomedicine, clinical medicine, material science and microelectronics.
authors.affiliation: Key Laboratory of Polar Materials and Devices, East China Normal University, Shanghai 200241, China. hyliu@ee.ecnu.edu.cn
authors.forename: Hong-Ying
authors.initials: HY
authors.lastname: Liu
authors.forename: Qing-Li
authors.initials: QL
authors.lastname: Li
authors.forename: Yi-Ting
authors.initials: YT
authors.lastname: Wang
authors.forename: Jin-Gao
authors.initials: JG
authors.lastname: Liu
authors.forename: Yong-Qi
authors.initials: YQ
authors.lastname: Xue
mesh_terms: D008853:Microscopy; D057054:Molecular Imaging / Q000295:instrumentation*; D055095:Optics and Photonics