title: Identification of genomic aberrations by array comparative genomic hybridization in patients with aortic dissections.
abstract: BACKGROUND
  The aim of the present study was to identify chromosomal loci that contribute to the pathogenesis of aortic dissection (AD) in a Korean population using array comparative genomic hybridization (CGH) and to confirm the results using real-time polymerase chain reaction (PCR).
  
  
  METHODS
  Eighteen patients with ADs were enrolled in this study. Genomic DNA was extracted from individual blood samples, and array CGH analyses were performed. Four corresponding genes with obvious genomic changes were analyzed using real-time PCR in order to assess the level of genomic imbalance identified by array CGH.
  
  
  RESULTS
  Genomic gains were most frequently detected at 8q24.3 (56%), followed by regions 7q35, 11q12.2, and 15q25.2 (50%). Genomic losses were most frequently observed at 4q35.2 (56%). Real-time PCR confirmed the results of the array CGH studies of the COL6A2, DGCR14, PCSK6, and SDHA genes.
  
  
  CONCLUSIONS
  This is the first study to identify candidate regions by array CGH in patients with ADs. The identification of genes that may predispose an individual to AD may lead to a better understanding of the mechanism of AD formation. Further multicenter studies comparing cohorts of patients of different ethnicities are warranted.
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, Incheon St. Mary's Hospital, The Catholic University of Korea, College of Medicine, Korea.
authors.forename: Jong Hui
authors.initials: JH
authors.lastname: Suh
authors.forename: Jeong-Seob
authors.initials: JS
authors.lastname: Yoon
authors.forename: Jong-Bum
authors.initials: JB
authors.lastname: Kwon
authors.forename: Hwan Wook
authors.initials: HW
authors.lastname: Kim
authors.forename: Young-Pil
authors.initials: YP
authors.lastname: Wang