title: Analysis of CD83 antigen expression in human breast fibroadenoma and adjacent tissue.
abstract: OBJECTIVE
  Dendritic cell maturation is considered essential for starting an immune response. The CD83 antigen is an important marker of dendritic cell maturation. The objectives here were to analyze CD83 antigen expression in human breast fibroadenoma and breast tissue adjacent to the lesion and to identify clinical factors that might influence this expression.
  
  
  METHODS
  This was a retrospective study at a public university hospital, in which 29 histopathological samples of breast fibroadenoma and adjacent breast tissue, from 28 women of reproductive age, were analyzed.
  
  
  METHODS
  The immunohistochemistry method was used to analyze the cell expression of the antigen. The antigen expression in the cells was evaluated by means of random manual counting using an optical microscope.
  
  
  RESULTS
  Positive expression of the CD83 antigen in the epithelial cells of the fibroadenoma (365.52; standard deviation ± 133.13) in relation to the adjacent breast tissue cells (189.59; standard deviation ± 140.75) was statistically larger (P < 0.001). Several clinical features were analyzed, but only parity was shown to influence CD83 antigen expression in the adjacent breast tissue, such that positive expression was more evident in nulliparous women (P = 0.042).
  
  
  CONCLUSIONS
  The expression of the CD83 antigen in the fibroadenoma was positive and greater than in the adjacent breast tissue. Positive expression of the antigen in the adjacent breast tissue was influenced by parity, and was significantly more evident in nulliparous women.
authors.affiliation: Department of Gynecology, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brazil. marcusnborges@yahoo.com.br
authors.forename: Marcus Nascimento
authors.initials: MN
authors.lastname: Borges
authors.forename: Gil
authors.initials: G
authors.lastname: Facina
authors.forename: Ismael Dale Cotrin Guerreiro
authors.initials: ID
authors.lastname: Silva
authors.forename: Angela Flávia Logullo
authors.initials: AF
authors.lastname: Waitzberg
authors.forename: Afonso Celso Pinto
authors.initials: AC
authors.lastname: Nazario
mesh_terms: D000293:Adolescent; D000328:Adult; D015703:Antigens, CD / Q000032:analysis*; D000951:Antigens, Neoplasm / Q000032:analysis*; D001940:Breast / Q000276:immunology* / Q000473:pathology; D001943:Breast Neoplasms / Q000276:immunology* / Q000473:pathology; D005260:Female; D018226:Fibroadenoma / Q000276:immunology* / Q000473:pathology; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis*; D008562:Membrane Glycoproteins / Q000032:analysis*; D008875:Middle Aged; D012189:Retrospective Studies; D055815:Young Adult; D000097802:CD83 Antigen
chemical_list: D015703:Antigens, CD; D000951:Antigens, Neoplasm; D007136:Immunoglobulins; D008562:Membrane Glycoproteins; D000097802:CD83 Antigen