title: Augmenter of liver regeneration may be a candidate for prognosis of HBV related acute-on-chronic liver failure as a regenerative marker.
abstract: OBJECTIVE
  To search for a new regenerative marker to estimate the prognosis of acute-on-chronic liver failure (ACLF).
  
  
  METHODS
  The CCl4 induced liver regeneration models were prepared and observed the change of ALR, hepatocyte growth factor (HGF), proliferation cell nuclear antigen (PCNA) and pathology. Meanwhile the sera of patients with HBV related liver disease were collected to examine the changes of ALR level and the prognosis of patients with ACLF was followed up.
  
  
  RESULTS
  After CCl4 injection, serum ALR level rose firstly and then declined in the ensuing 12 hours to near-basal level (F=30.495, p<0.01). ALR level in the liver tissue showed an inverse pattern. The changes of PCNA, HGF and pathology showed a consistent trend with serum ALR level. Serum ALR level was higher in ACLF (n=20) and hepatocellular carcinoma (n=20) than in normal control (n=10) (2.68±1.95 vs. 0.74±0.31, p<0.01; 1.77±1.32 vs. 0.74±0.31, p=0.035). Serum ALR level of patients with ACLF was more significant in survival group (n=10) than in dead group (n=10) in early stage of disease (7.83±1.77 vs. 2.14±1.58, t=7.576, p<0.01).
  
  
  CONCLUSIONS
  ALR level in serum may indicate hepatocyte proliferation or liver regeneration. High ALR level in serum in early stage of ACLF may mean a good prognosis.
authors.affiliation: Beijing Artificial Liver Treatment and Training Centre, Beijing Youan Hospital, Affiliated Hospital of Capital Medical University, Beijing, China.
authors.forename: Shi
authors.initials: S
authors.lastname: Hongbo
authors.forename: Chen
authors.initials: C
authors.lastname: Yu
authors.forename: Kong
authors.initials: K
authors.lastname: Ming
authors.forename: Shi
authors.initials: S
authors.lastname: Honglin
authors.forename: Han Yuan
authors.initials: HY
authors.lastname: Ping
authors.forename: Duan Zhong
authors.initials: DZ
authors.lastname: Ping
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D015415:Biomarkers / Q000097:blood; D002251:Carbon Tetrachloride; D016022:Case-Control Studies; D056486:Chemical and Drug Induced Liver Injury / Q000097:blood* / Q000209:etiology / Q000473:pathology; D002681:China; D004195:Disease Models, Animal; D058625:End Stage Liver Disease / Q000097:blood* / Q000473:pathology / Q000821:virology; D006509:Hepatitis B / Q000150:complications*; D017228:Hepatocyte Growth Factor / Q000097:blood; D006801:Humans; D008099:Liver / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D017114:Liver Failure, Acute / Q000097:blood* / Q000473:pathology / Q000821:virology; D008115:Liver Regeneration; D008297:Male; D011379:Prognosis; D018809:Proliferating Cell Nuclear Antigen / Q000097:blood; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D015854:Up-Regulation; D050862:Oxidoreductases Acting on Sulfur Group Donors
chemical_list: D015415:Biomarkers; D002251:Carbon Tetrachloride; D017228:Hepatocyte Growth Factor; D018809:Proliferating Cell Nuclear Antigen; D011506:Proteins; C066855:HGF protein, human; C088825:GFER protein, rat; D050862:Oxidoreductases Acting on Sulfur Group Donors