title: Towards a cellular multi-parameter analysis platform: fluorescence in situ hybridization (FISH) on microhole-array chips.
abstract: Highly-sensitive analysis systems based on cellular multi-parameter are needed in the diagnostics. Therefore we improved our previously developed chip platform for another additional analysis method, the fluorescence in situ hybridization. Fluorescence in situ hybridization (FISH) is a technique used in the diagnostics to determine the localization and the presence or absence of specific DNA sequence. To improve this labor- and cost-intensive method, we reduced the assay consumption by a factor of 5 compared to the standard protocol. Microhole chips were used for making the cells well addressable. The chips were fabricated by semiconductor technology on the basis of a Silicon wafer with a thin deposited silicon nitride layer (Si(3)N(4)). Human retina pigment epithelia (ARPE-19) cells were arrayed on 5-μm holes of a 35 × 35 microhole-array by a gently negative differential pressure of around 5 mbar. After 3 hours of incubation the cells were attached to the chip and the FISH protocol was applied to the positioned cells. A LabView software was developed to simplify the analysis. The software automatically counts the number of dots (positive labeled chromosome regions) as well as the distance between adjacent dots. Our developed platform reduces the assay consumption and the labor time. Furthermore, during the 3 hours of incubation non-invasive or minimal-invasive methods like Raman- and impedance-spectroscopy can be applied.
authors.affiliation: Department Biomedical Microsystems, Fraunhofer Insitute for Biomedical Engineering.
authors.forename: Christian M
authors.initials: CM
authors.lastname: Kurz
authors.forename: Stefan V D
authors.initials: SV
authors.lastname: Moosdijk
authors.forename: Hagen
authors.initials: H
authors.lastname: Thielecke
authors.forename: Thomas
authors.initials: T
authors.lastname: Velten
mesh_terms: D002460:Cell Line; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D006801:Humans; D007091:Image Processing, Computer-Assisted; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008027:Light; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D008856:Microscopy, Fluorescence; D012984:Software; D014966:Xanthenes / Q000737:chemistry
chemical_list: D014966:Xanthenes; C034657:Texas red; D016650:Fluorescein-5-isothiocyanate