title: [Preliminary study on detection of Echinococcus granulosus DNA by loop-mediated isothermal amplification].
abstract: OBJECTIVE
  To evaluate the sensitivity of loop-mediated isothermal amplification (LAMP) on the detection of Echinococcus granulosus.
  
  
  METHODS
  The DNAs were extracted from Echinococcus granulosus eggs and adults. According to Echinococcus mitochondrial 12S rRNA sequences and the mechanism of LAMP, 4 Echinococcus specific primers were designed and used for LAMP assay, and Bubble taenia and the blank were used as the negative control for evaluation of the specificity. The LAMP products were stained by SYBR Green I and analyzed by electrophoresis, and 1000, 100, 10, 1 eggs of Echinococcus granulosus per 200 microl were amplified by LAMP for evaluating the sensitivity.
  
  
  RESULTS
  The LAMP products of Echinococcus granulosus adult DNA became turbid and green after staining while the products of control DNA kept clarify and brown after staining. Electrophoresis analysis showed that the LAMP products of Echinococcus granulosus eggs presented characteristic ladders, but the products of control did not. The detection limit of LAMP assay was 1 egg of Echinococcus granulosus per reaction.
  
  
  CONCLUSIONS
  LAMP assay is a simple, sensitive and specific method for detection of hydatid disease pathogens and could be used for the disease surveillance.
authors.affiliation: Jiangsu Institute of Parasitic Diseases, Wuxi 214064, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Xiang-Zhen
authors.forename: Jin
authors.initials: J
authors.lastname: Xiao-Lin
authors.forename: Li
authors.initials: L
authors.lastname: Jian
authors.forename: Jiang
authors.initials: J
authors.lastname: Wen-Cai
authors.forename: Jiang
authors.initials: J
authors.lastname: Gang
mesh_terms: D000818:Animals; D018509:DNA, Helminth / Q000032:analysis*; D048209:Echinococcus granulosus / Q000235:genetics*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D018509:DNA, Helminth