title: Protein misfolding, mitochondrial dysfunction and muscle loss are not directly dependent on soluble and aggregation state of mSOD1 protein in skeletal muscle of ALS.
abstract: Mutant superoxide dismutase 1 (mSOD1) is often found as aggregates at the outer-membrane of mitochondria in motor neurons of various mouse models and familial amyotrophic lateral sclerosis (f-ALS) patients. It has been postulated that disruption of mitochondrial function by physical association of misfolded mSOD1 aggregates may actually be the trigger for initiation of degeneration of motor neurons in ALS. However, it was not clear if the same mechanism is involved in muscle degeneration and mitochondrial dysfunction in skeletal muscles of ALS. Recent study from our laboratory show that two skeletal muscle proteins, namely creatine kinase (CK) and glyceraldehydes-3-phosphate dehydrogenase (GAPDH) undergo major conformational and functional changes in the f-ALS mouse model of ALS (G93A). In this paper, we report two intriguing observations which are as follows:(i) G93A protein does not form aggregates in skeletal muscle at any stages of disease process probably due to high chymotrypsin-like activity of proteasome and thus G93A protein aggregates have no direct effects on progressive loss of muscle mass and global changes in protein conformation in ALS, and (ii) the soluble G93A protein does not have direct effects on mitochondrial dysfunction as determined by quantifying the release of reactive oxygen species (ROS) in skeletal muscle mitochondria; instead, the proteins affected by G93A possibly affect mitochondrial ROS release. These data strongly suggest for the first time that unlike in motor neurons, the soluble and aggregation states of the G93A protein do not have direct effects on protein misfolding and mitochondrial dysfunction in skeletal muscle during ALS.
authors.affiliation: Barshop Institute for Longevity and Aging Studies, University of Texas Health Science Center at San Antonio, TX 78229-3900, USA.
authors.forename: Rochelle
authors.initials: R
authors.lastname: Wei
authors.forename: Arunabh
authors.initials: A
authors.lastname: Bhattacharya
authors.forename: Naveen
authors.initials: N
authors.lastname: Chintalaramulu
authors.forename: Amanda L
authors.initials: AL
authors.lastname: Jernigan
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Liu
authors.forename: Holly
authors.initials: H
authors.lastname: Van Remmen
authors.forename: Asish R
authors.initials: AR
authors.lastname: Chaudhuri
mesh_terms: D000690:Amyotrophic Lateral Sclerosis / Q000201:enzymology*; D000818:Animals; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D028361:Mitochondrial Diseases / Q000201:enzymology*; D018482:Muscle, Skeletal / Q000201:enzymology*; D017510:Protein Folding; D012995:Solubility; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*
chemical_list: C109415:SOD1 G93A protein; D013482:Superoxide Dismutase