title: Identification of divergently selected regions between Japanese Black and Holstein cattle using bovine 50k SNP array.
abstract: Differences between average allelic frequencies of genes that relate to traits suggest that it would be evidence of artificial selections. Sliding window approach is a useful method to identify genomic regions that have been differently selected between two breeds. The objective of this study was to identify the divergently selected regions between Japanese Black (JB) and Japanese Holstein (JH) cattle based on genotypic information obtained through a high-density single nucleotide polymorphism (SNP) panel. After genotyping of 54 001 SNP markers on 100 animals (50 JB and 50 JH), 40 635 SNPs were suitable for the analysis. For each of these SNPs, the absolute difference between allelic frequencies of JB and JH was calculated. In the current study, 10 consecutive SNPs were defined as components of a window. For each window, the average difference in allelic frequency was calculated. This was termed sliding window average difference (SWAD). Among 40 055 windows, we focused on 39 windows with the largest SWAD. This was equivalent to 0.1% of all windows and the SWAD was more than 0.435. Some of these windows overlapped and were distributed in 11 regions. These regions were in good agreement with reported quantitative trait locus, therefore would be selection signatures and good candidates that harbor the causative mutations.
authors.affiliation: Laboratory of Animal Breeding and Genetics, Graduate School of Agricultural Science, Kobe University, Nada, Kobe             Food Resources Education & Research Center, Kobe University, Kasai, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Hosokawa
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ishii
authors.forename: Keita
authors.initials: K
authors.lastname: Yamaji
authors.forename: Shinji
authors.initials: S
authors.lastname: Sasazaki
authors.forename: Kenji
authors.initials: K
authors.lastname: Oyama
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Mannen
mesh_terms: D000818:Animals; D001947:Breeding; D002417:Cattle / Q000235:genetics*; D005787:Gene Frequency; D016678:Genome / Q000235:genetics; D005838:Genotype; D060005:Genotyping Techniques / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci / Q000235:genetics; D012641:Selection, Genetic