title: [The expression of myosin heavy chain isoforms of human normal laryngeal muscles and the difference between the adductor and abductor].
abstract: OBJECTIVE
  To investigate the expression of myosin heavy chain (MHC) in normal laryngeal muscle and the difference between the adductor and abductor.
  
  
  METHODS
  Seven patients with total laryngectomy were enrolled in this study. The adductor muscles were acquired from the lateral cricoarytenoid (LCA) muscle and the abductor muscles were acquired from the posterior cricoarytenoid (PCA) muscle. The expression of myosin heavy chain were detected with fluorescent quantitative reverse transcription polymerase chain reaction (RT-PCR) and immunofluorescence staining respectively.
  
  
  RESULTS
  (1) MHC-II b was expressed in laryngeal muscles at mRNA levels, and not expressed at the protein level; (2) At both mRNA level and protein level, the expression of MHC-I was higher in the PCA muscles than in the LCA muscles while MHC-II level was higher in the LCA muscles than in the PCA muscles.
  
  
  CONCLUSIONS
  (1) MHC-II b protein was not expressed in human laryngeal muscles; (2) Phenotypic differences were significant in laryngeal adductor and abductor muscles based on their different functions. PCA contained larger percentage of MHC-I fibers, while LCA contained more MHC-II fibers.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Changhai Hospital, the First Affiliated Hospital of Second Military Medical University, Shanghai, 200433, China.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Qiu
authors.forename: Hongliang
authors.initials: H
authors.lastname: Zheng
authors.forename: Shicai
authors.initials: S
authors.lastname: Chen
authors.forename: Donghui
authors.initials: D
authors.lastname: Chen
authors.forename: Jianqiu
authors.initials: J
authors.lastname: Chen
authors.forename: Siwen
authors.initials: S
authors.lastname: Xia
mesh_terms: D000368:Aged; D005260:Female; D006801:Humans; D007821:Laryngeal Muscles / Q000737:chemistry* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D018995:Myosin Heavy Chains / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D020033:Protein Isoforms
chemical_list: D020033:Protein Isoforms; D018995:Myosin Heavy Chains