title: Urotensin II and urocortin trigger the expression of myostatin, a negative regulator of cardiac growth, in cardiomyocytes.
abstract: Urotensin II (UII) and urocortin (UCN) are potent contributors to the physiopathology of heart failure. Our study investigated the effects of UII and UCN on the expression of myostatin (Mstn) in primary culture of adult cardiomyocytes. Adult rat cardiomyocytes were stimulated for 48 h with UII and UCN. Cell size and protein content were determined. Mstn gene expression was determined by real time quantitative polymerase chain reaction. Treatment with UII and UCN stimulates hypertrophy of adult cardiomyocytes. This effect was associated with a twofold increase of Mstn gene expression. We have established for the first time that the two hypertrophic peptides UII and UCN stimulate the expression of Mstn.
authors.affiliation: Pôle de recherche en Endocrinologie, Diabète et Nutrition, Institut de Recherche Expérimentale et Clinique, Cliniques Universitaires St-Luc and Université Catholique de Louvain, Brussels, Belgium. damien.gruson@uclouvain.be
authors.forename: Damien
authors.initials: D
authors.lastname: Gruson
authors.forename: Audrey
authors.initials: A
authors.lastname: Ginion
authors.forename: Pascale
authors.initials: P
authors.lastname: Lause
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Ketelslegers
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Thissen
authors.forename: Luc
authors.initials: L
authors.lastname: Bertrand
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D015870:Gene Expression; D005786:Gene Expression Regulation; D008297:Male; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000378:metabolism*; D055435:Myostatin / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D054832:Urocortins / Q000502:physiology*; D014579:Urotensins / Q000502:physiology*
chemical_list: C525913:Mstn protein, rat; D055435:Myostatin; D054832:Urocortins; D014579:Urotensins; C029677:urotensin II