title: Activity, expression and genetic variation of canine β-defensin 103: a multifunctional antimicrobial peptide in the skin of domestic dogs.
abstract: The skin functions as more than a physical barrier to infection. Epithelial cells of the skin can synthesize antimicrobial peptides, including defensins, which exhibit direct antimicrobial activity. Here we characterize the expression pattern, genetic variation and activity of the major β-defensin expressed in canine skin, canine β-defensin 103 (CBD103). The gene encoding CBD103 exhibits two forms of polymorphism: a common 3-basepair deletion allele and a gene copy-number variation. Golden retrievers and Labrador retrievers were the only breeds that encoded the variant allele of CBD103, termed CBD103ΔG23. Both these breeds also exhibited a CBD103 gene copy-number polymorphism that ranged from 2 to 4 gene-copies per diploid genome. Recombinant CBD103 and CBD103ΔG23, as well as the human ortholog human β-defensin 3 (hBD3) and hBD3ΔG23, showed potent and comparable antimicrobial killing against both methicillin-susceptible and methicillin-resistant Staphylococcus pseudintermedius. Skin biopsy specimens from dogs with atopic dermatitis revealed CBD103 expression levels similar to those in healthy controls and comparable at lesional and nonlesional sites. This expression pattern in dogs differs from the previously reported reduced expression of the human ortholog in atopic dermatitis. Overall, the similarities of CBD103 and its human ortholog reported here support the notion that the domestic dog may serve as a valuable model for studying β-defensin biology in the skin.
authors.affiliation: Department of Microbiology and Immunology, UC Davis School of Medicine, Davis, CA 95616-8645, USA.
authors.forename: Brian C
authors.initials: BC
authors.lastname: Leonard
authors.forename: Stanley L
authors.initials: SL
authors.lastname: Marks
authors.forename: Catherine A
authors.initials: CA
authors.lastname: Outerbridge
authors.forename: Verena K
authors.initials: VK
authors.lastname: Affolter
authors.forename: Anchasa
authors.initials: A
authors.lastname: Kananurak
authors.forename: Amy
authors.initials: A
authors.lastname: Young
authors.forename: Peter F
authors.initials: PF
authors.lastname: Moore
authors.forename: Danika L
authors.initials: DL
authors.lastname: Bannasch
authors.forename: Charles L
authors.initials: CL
authors.lastname: Bevins
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000276:immunology / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D001947:Breeding; D004252:DNA Mutational Analysis; D003876:Dermatitis, Atopic / Q000235:genetics / Q000276:immunology*; D004285:Dogs / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D018628:Gene Dosage; D020869:Gene Expression Profiling; D006801:Humans; D023421:Models, Animal; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D016415:Sequence Alignment; D017384:Sequence Deletion / Q000235:genetics; D012867:Skin / Q000276:immunology*; D013045:Species Specificity; D013207:Staphylococcal Skin Infections / Q000235:genetics / Q000276:immunology; D023083:beta-Defensins / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D023083:beta-Defensins