title: Effect of different iron loads on serum and tissue biochemical parameters and liver hepcidin mRNA abundance of neonatal piglets.
abstract: Iron (Fe) is an essential and important trace element for animals. In order to study its metabolism and relationship with hepcidin, piglet models of Fe-deficiency and Fe-overload were established by intramuscular injection with different doses of Fe-dextran (150 mg Fe/ml) within 1 week of age. Twelve piglets were divided into three groups of four animals: deficiency, regular and overload group, receiving 0 ml, 1 ml and 6 ml Fe-dextran, respectively. The piglets were euthanised at the age of 7 days for analysis. The results showed that the Fe-concentrations in liver, spleen and serum of piglets in the overload group were higher than in the regular and deficiency groups (p < 0.05). In the overload group, several serum biochemical parameters, e.g. globulin, total bilirubin, total cholesterol, high density lipoprotein (HDL), malondialdehyde, glutathione peroxidase (GPx), peroxidase and xanthine oxidase were higher, while alkaline phosphatase (AKP) and triglycerides were lower, compared with the regular group (p < 0.05). The serum concentrations of AKP, total bilirubin and peroxidase in the deficiency group were lower, while HDL and GPx were higher, compared with the regular group (p < 0.05). Hepcidin mRNA abundance was 131 times lower in the liver of piglets with Fe-deficiency, and 7 times higher in the overloaded group than that in the regular group (p < 0.05). In conclusion, Fe-overload and deficiency would influence Fe-metabolism, serum biochemical indexes, oxidation state and hepcidin mRNA abundance in piglet liver.
authors.affiliation: Department of Animal Science, College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, PR China.
authors.forename: Mengyun
authors.initials: M
authors.lastname: Lia
authors.forename: Qingqiang
authors.initials: Q
authors.lastname: Yin
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Dang
authors.forename: Juan
authors.initials: J
authors.lastname: Chang
authors.forename: Ruiyu
authors.initials: R
authors.lastname: Zuo
authors.forename: Qiuhong
authors.initials: Q
authors.lastname: Zheng
mesh_terms: D000821:Animal Feed; D000818:Animals; D000831:Animals, Newborn; D023181:Antimicrobial Cationic Peptides / Q000235:genetics / Q000378:metabolism*; D000975:Antioxidants / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects*; D064451:Hepcidins; D007501:Iron / Q000097:blood / Q000378:metabolism; D007505:Iron-Dextran Complex / Q000008:administration & dosage / Q000009:adverse effects*; D008099:Liver / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D013154:Spleen / Q000737:chemistry / Q000378:metabolism; D013552:Swine / Q000378:metabolism*
chemical_list: D023181:Antimicrobial Cationic Peptides; D000975:Antioxidants; D064451:Hepcidins; D012333:RNA, Messenger; D007505:Iron-Dextran Complex; D007501:Iron