title: H2B ubiquitylation controls the formation of export-competent mRNP.
abstract: Histone H2B ubiquitylation is a transcription-dependent modification that not only regulates nucleosome dynamics but also controls the trimethylation of histone H3 on lysine 4 by promoting ubiquitylation of Swd2, a component of both the histone methyltransferase COMPASS complex and the cleavage and polyadenylation factor(CPF). We show that preventing either H2B ubiquitylation or H2B-dependent modification of Swd2 results in nuclear accumulation of poly(A) RNA due to a defect in the integrity and stability of APT, a subcomplex of the CPF. Ubiquitin-regulated APT complex dynamics is required for the correct recruitment of the mRNA export receptor Mex67 to nuclear mRNPs. While H2B ubiquitylation controls the recruitment of the different Mex67 adaptors to mRNPs, the effect of Swd2 ubiquitylation is restricted to Yra1 and Nab2, which, in turn, controls poly(A) tail length. Modification of H2B thus participates in the crosstalk between cotranscriptional events and assembly of mRNPs linking nuclear processing and mRNA export.
authors.affiliation: Institut Jacques Monod, Université Paris Diderot, CNRS, Bâtiment Buffon, 15 rue Hélène Brion, 75205 Paris Cedex 13, France.
authors.forename: Adeline
authors.initials: A
authors.lastname: Vitaliano-Prunier
authors.forename: Anna
authors.initials: A
authors.lastname: Babour
authors.forename: Lucas
authors.initials: L
authors.lastname: Hérissant
authors.forename: Luciano
authors.initials: L
authors.lastname: Apponi
authors.forename: Thanasis
authors.initials: T
authors.lastname: Margaritis
authors.forename: Frank C P
authors.initials: FC
authors.lastname: Holstege
authors.forename: Anita H
authors.initials: AH
authors.lastname: Corbett
authors.forename: Carole
authors.initials: C
authors.lastname: Gwizdek
authors.forename: Catherine
authors.initials: C
authors.lastname: Dargemont
mesh_terms: D021581:Active Transport, Cell Nucleus; D002467:Cell Nucleus / Q000378:metabolism; D011495:Histone-Lysine N-Methyltransferase / Q000378:metabolism; D006657:Histones / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism; D029543:Nucleocytoplasmic Transport Proteins / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000378:metabolism; D012261:Ribonucleoproteins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D054875:Ubiquitination
chemical_list: D006657:Histones; C080773:NAB2 protein, S cerevisiae; D009687:Nuclear Proteins; D029543:Nucleocytoplasmic Transport Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; D029701:Saccharomyces cerevisiae Proteins; C105887:YRA1 protein, S cerevisiae; C026452:messenger ribonucleoprotein; D011495:Histone-Lysine N-Methyltransferase; C484873:SWD2 protein, S cerevisiae