title: To study various concentrations of magnesium and aluminium on amylin hormone conformation.
abstract: Type 2 diabetes mellitus can be defined as a conformational disease since a beta cell producing protein called amylin undergoes a change in the tertiary structure followed by self-aggregation and deposition. Amylin deposition causes destruction of pancreatic beta-cells. The aim of this study was to investigate whether different concentrations of magnesium and aluminium alter amylin conformation under near-physiological circumstances. Conformational variations were monitored by fluorescent method before and after incubation by shaker incubator in 37 degrees C by LS55 spectrofluorometer instrument. This in vitro study showed that magnesium had contradictory effects on amylin folding and these effects were magnesium concentration dependent. Magnesium with concentration of 1 to 1.5 mM had inhibitory effect but in 2.5 to 3.5 microM promoted amylin misfolding significantly (p < 0.05). The obtained data also demonstrated that aluminium with concentrations of 5, 10 and 20 microM had stimulatory effects on formation of beta-amyloid sheet significantly (p < 0.05). It may be concluded that islet amyloid misfolding and cytotoxicity to beta-cells might be magnesium dose dependent in diabetic patients.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Islamic Republic of Iran.
authors.forename: S M
authors.initials: SM
authors.lastname: Mirhashemi
authors.forename: M H
authors.initials: MH
authors.lastname: Aarabi
mesh_terms: D000535:Aluminum / Q000737:chemistry*; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D006801:Humans; D058228:Islet Amyloid Polypeptide / Q000737:chemistry*; D008274:Magnesium / Q000737:chemistry*; D011487:Protein Conformation; D017510:Protein Folding
chemical_list: D058228:Islet Amyloid Polypeptide; D000535:Aluminum; D008274:Magnesium