title: Applications and perspectives of multi-parameter flow cytometry to microbial biofuels production processes.
abstract: Conventional microbiology methods used to monitor microbial biofuels production are based on off-line analyses. The analyses are, unfortunately, insufficient for bioprocess optimization. Real time process control strategies, such as flow cytometry (FC), can be used to monitor bioprocess development (at-line) by providing single cell information that improves process model formulation and validation. This paper reviews the current uses and potential applications of FC in biodiesel, bioethanol, biomethane, biohydrogen and fuel cell processes. By highlighting the inherent accuracy and robustness of the technique for a range of biofuel processing parameters, more robust monitoring and control may be implemented to enhance process efficiency.
authors.affiliation: Laboratório Nacional de Energia e Geologia, I.P., Unidade de Bioenergia, Estrada do Paço do Lumiar 22, 1649-038 Lisboa, Portugal. teresa.lopesilva@lneg.pt
authors.forename: Teresa Lopes
authors.initials: TL
authors.lastname: da Silva
authors.forename: José Carlos
authors.initials: JC
authors.lastname: Roseiro
authors.forename: Alberto
authors.initials: A
authors.lastname: Reis
mesh_terms: D056804:Biofuels; D005434:Flow Cytometry / Q000379:methods*; D007218:Industrial Microbiology / Q000379:methods*
chemical_list: D056804:Biofuels