title: MiR-200a is involved in rat epididymal development by targeting β-catenin mRNA.
abstract: The expression of 350 microRNAs (miRNAs) in epididymis of rat from postnatal development to adult (from postnatal days 7-70) was profiled with home-made miRNA microarray. Among them, 48 miRNAs changed significantly, in which the expression of miR-200a increased obviously with time, in a good agreement with that obtained from northern blot analysis. The real-time quantitative-polymerase chain reaction result indicated that temporal expression of rat β-catenin was exactly inversed to that of miR-200a during rat epididymal development, implying that miR-200a might also target β-catenin mRNA in rat epididymis as reported by Saydam et al. in humans. The bioinformatic analysis indicated that 3' untranslated region of rat β-catenin mRNA did contain a putative binding site for miR-200a. Meanwhile, it was found that the sequence of this binding site was different from that of human β-catenin mRNA with a deletion of two adjacent nucleotides (U and C). But the results of luciferase targeting assay in HEK 293T cells and the overexpression of miR-200a in rat NRK cells demonstrated that miR-200a did target rat β-catenin mRNA and cause the suppression of its expression. All these results show that miR-200a should be involved in rat epididymal development by targeting β-catenin mRNA of rat and suppressing its expression.
authors.affiliation: State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, China.
authors.forename: Xiaojiang
authors.initials: X
authors.lastname: Wu
authors.forename: Botao
authors.initials: B
authors.lastname: Zhao
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Yue
authors.initials: Y
authors.lastname: Chen
authors.forename: Ruqiang
authors.initials: R
authors.lastname: Liang
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.forename: Youxin
authors.initials: Y
authors.lastname: Jin
authors.forename: Kangcheng
authors.initials: K
authors.lastname: Ruan
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D016000:Cluster Analysis; D019295:Computational Biology / Q000379:methods; D004822:Epididymis / Q000378:metabolism*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D057809:HEK293 Cells; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis*; D008957:Models, Genetic; D008969:Molecular Sequence Data; D051381:Rats; D051176:beta Catenin / Q000378:metabolism*
chemical_list: C555600:MIRN200 microRNA, rat; D035683:MicroRNAs; D051176:beta Catenin