title: Selection of distinct strain phenotypes in mice infected by ovine natural scrapie isolates similar to CH1641 experimental scrapie.
abstract: A few cases of transmissible spongiform encephalopathies in sheep have been described in France in which the protease-resistant prion protein (PrP(res)) exhibited some features in Western blot of experimental bovine spongiform encephalopathy in sheep. Their molecular characteristics were indistinguishable from those produced in the CH1641 experimental scrapie isolate. Four of these CH1641-like isolates were inoculated intracerebrally into wild-type C57Bl/6 mice. In striking contrast to previous results in ovine transgenic mice, CH1641 transmission in wild-type mice was efficient. Several components of the strain signature, that is, PrP(res) profile, brain distribution, and morphology of the deposits of the disease-associated prion protein, had some similarities with "classical" scrapie and clearly differed from both bovine spongiform encephalopathy in sheep and CH1641 transmission in ovine transgenic mice. These results on CH1641-like isolates in wild-type mice may be consistent with the presence in these isolates of mixed conformers with different abilities to propagate and mediate specific disease phenotypes in different species.
authors.affiliation: Neurodegenerative Diseases Unit, ANSES-Lyon, Lyon, France.
authors.forename: Johann
authors.initials: J
authors.lastname: Vulin
authors.forename: Katy E
authors.initials: KE
authors.lastname: Beck
authors.forename: Anna
authors.initials: A
authors.lastname: Bencsik
authors.forename: Latefa
authors.initials: L
authors.lastname: Lakhdar
authors.forename: John
authors.initials: J
authors.lastname: Spiropoulos
authors.forename: Thierry
authors.initials: T
authors.lastname: Baron
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D023421:Models, Animal; D010641:Phenotype; D018620:PrPSc Proteins / Q000235:genetics* / Q000378:metabolism*; D012608:Scrapie / Q000235:genetics* / Q000473:pathology; D012756:Sheep / Q000235:genetics; D013154:Spleen / Q000378:metabolism / Q000473:pathology
chemical_list: D018620:PrPSc Proteins