title: Induction of apoptosis in human breast adenocarcinoma cells MCF-7 by monapurpyridine A, a new azaphilone derivative from Monascus purpureus NTU 568.
abstract: A new azaphilonidal derivative, monapurpyridine A (MPA), has recently been isolated from the fermented products of Monascus purpureus NTU 568. The structure of MPA was elucidated by nuclear magnetic resonance (1H-NMR, 13C-NMR, COSY, HMQC, and HMBC) and other spectroscopic analyses. Biological evaluation revealed that MPA could induce cell death in human breast adenocarcinoma cells MCF-7, and it has no significant toxicity to normal mammary epithelial cells M10. The MTT assay and flow cytometric analysis were employed to investigate cell viability and cell cycle influenced by MPA. Moreover, we used Western blot and caspase activity assay to demonstrate the activation of caspase-3, -8 and -9 resulted from MPA. All evidence supported that MPA was suitable for developing into a chemotherapeutic or chemopreventive agent against breast cancer.
authors.affiliation: Department of Biochemical Science and Technology, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Li-Chuan
authors.initials: LC
authors.lastname: Hsu
authors.forename: Ya-Wen
authors.initials: YW
authors.lastname: Hsu
authors.forename: Yu-Han
authors.initials: YH
authors.lastname: Liang
authors.forename: Chia-Ching
authors.initials: CC
authors.lastname: Liaw
authors.forename: Yao-Haur
authors.initials: YH
authors.lastname: Kuo
authors.forename: Tzu-Ming
authors.initials: TM
authors.lastname: Pan
mesh_terms: D000230:Adenocarcinoma; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001943:Breast Neoplasms; D020169:Caspases / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003470:Culture Media / Q000737:chemistry; D004789:Enzyme Activation; D005260:Female; D005285:Fermentation; D006801:Humans; D015394:Molecular Structure; D032741:Monascus / Q000378:metabolism*; D012275:Oryza / Q000737:chemistry; D011725:Pyridines / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D003470:Culture Media; D011725:Pyridines; C571654:monapurpyridine A; D020169:Caspases