title: Detection of minor subpopulations of colorectal adenocarcinoma cells expressing cancer stem cell markers.
abstract: The expression of puitative surface molecular markers of cancer stem cells on human colorectal adenocarcinoma cells was analyzed by flow cytofluorometry. Cell subpopulations expressing markers of epithelial and malignant cells and stem cell markers were identified. Four minor subpopulations with CD24(+)/CD133(+), CD44(+)/CD133(+), CD90(+)/CD71(+), or CD90(+)/CD24(+) phenotypes meeting this requirement were detected; presumably, those were cancer stem cell subpopulations. These results extend our knowledge on heterogeneity of human colorectal adenocarcinoma cell population and outline new trends of research of cancer stem cell phenotype in these tumors.
authors.affiliation: V. N. Orekhovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow, Russia. wonderland85@mail.ru
authors.forename: A M
authors.initials: AM
authors.lastname: Gisina
authors.forename: A Yu
authors.initials: AY
authors.lastname: Lupatov
authors.forename: P A
authors.initials: PA
authors.lastname: Karalkin
authors.forename: O A
authors.initials: OA
authors.lastname: Mainovskaya
authors.forename: L O
authors.initials: LO
authors.lastname: Petrov
authors.forename: D V
authors.initials: DV
authors.lastname: Sidorov
authors.forename: V N
authors.initials: VN
authors.lastname: Yarygin
authors.forename: K N
authors.initials: KN
authors.lastname: Yarygin
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology*; D015703:Antigens, CD / Q000378:metabolism*; D000951:Antigens, Neoplasm / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000378:metabolism / Q000473:pathology*; D000071858:Epithelial Cell Adhesion Molecule; D005434:Flow Cytometry; D006801:Humans; D014411:Neoplastic Stem Cells / Q000378:metabolism*
chemical_list: D015703:Antigens, CD; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D015815:Cell Adhesion Molecules; C499379:EPCAM protein, human; D000071858:Epithelial Cell Adhesion Molecule