title: Simultaneous determination of zolazepam and tiletamine in dog plasma by liquid chromatography coupled to a tandem mass spectrometry.
abstract: A mixture of tiletamine, a dissociative anesthetic, and zolazepam, a minor tranquilizer, has been widely used as an anesthetic or an immobilizing agent in a variety of animal species. However, interestingly, their pharmacokinetic behaviors have been published only in polar bears and pigs. In this study, we introduce a sensitive liquid chromatography/tandem mass spectrometry (LC/MS/MS) method for determining the two drugs in dog plasma. After simple protein precipitation with acetonitrile including midazolam (internal standard), the analytes were chromatographed on a reversed-phase column with a mobile phase of 10 m m ammonium acetate aqueous solution and acetonitrile (1:4, v/v). The accuracy and precision of the assay were in accordance with FDA regulations for the validation of bioanalytical methods. This method was used to measure the concentrations of zolazepam and tiletamine in plasma after a single intramuscular 10 mg dose of each in beagle dogs.
authors.affiliation: College of Pharmacy, Yeungnam University, Kyoungbuk, South Korea.
authors.forename: Kyeumhan
authors.initials: K
authors.lastname: Noh
authors.forename: Kil-Soo
authors.initials: KS
authors.lastname: Kim
authors.forename: Byoungki
authors.initials: B
authors.lastname: Ahn
authors.forename: Pillippe
authors.initials: P
authors.lastname: Archimbault
authors.forename: Tae-Ho
authors.initials: TH
authors.lastname: Oh
authors.forename: Wonku
authors.initials: W
authors.lastname: Kang
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D004285:Dogs; D004355:Drug Stability; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013992:Tiletamine / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D015041:Zolazepam / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics
chemical_list: D013992:Tiletamine; D015041:Zolazepam