title: Axonal transcription factors signal retrogradely in lesioned peripheral nerve.
abstract: Retrograde axonal injury signalling stimulates cell body responses in lesioned peripheral neurons. The involvement of importins in retrograde transport suggests that transcription factors (TFs) might be directly involved in axonal injury signalling. Here, we show that multiple TFs are found in axons and associate with dynein in axoplasm from injured nerve. Biochemical and functional validation for one TF family establishes that axonal STAT3 is locally translated and activated upon injury, and is transported retrogradely with dynein and importin α5 to modulate survival of peripheral sensory neurons after injury. Hence, retrograde transport of TFs from axonal lesion sites provides a direct link between axon and nucleus.
authors.affiliation: Department of Biological Chemistry, Weizmann Institute of Science, Rehovot, Israel.
authors.forename: Keren
authors.initials: K
authors.lastname: Ben-Yaakov
authors.forename: Shachar Y
authors.initials: SY
authors.lastname: Dagan
authors.forename: Yael
authors.initials: Y
authors.lastname: Segal-Ruder
authors.forename: Ophir
authors.initials: O
authors.lastname: Shalem
authors.forename: Deepika
authors.initials: D
authors.lastname: Vuppalanchi
authors.forename: Dianna E
authors.initials: DE
authors.lastname: Willis
authors.forename: Dmitry
authors.initials: D
authors.lastname: Yudin
authors.forename: Ida
authors.initials: I
authors.lastname: Rishal
authors.forename: Franziska
authors.initials: F
authors.lastname: Rother
authors.forename: Michael
authors.initials: M
authors.lastname: Bader
authors.forename: Armin
authors.initials: A
authors.lastname: Blesch
authors.forename: Yitzhak
authors.initials: Y
authors.lastname: Pilpel
authors.forename: Jeffery L
authors.initials: JL
authors.lastname: Twiss
authors.forename: Mike
authors.initials: M
authors.lastname: Fainzilber
mesh_terms: D000818:Animals; D001370:Axonal Transport / Q000502:physiology; D001369:Axons / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D004398:Dyneins / Q000378:metabolism; D005727:Ganglia, Spinal / Q000378:metabolism*; D028884:Karyopherins / Q000378:metabolism; D008297:Male; D051379:Mice; D059348:Peripheral Nerve Injuries / Q000378:metabolism*; D021381:Protein Transport / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D050796:STAT3 Transcription Factor / Q000378:metabolism; D011984:Sensory Receptor Cells / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D028884:Karyopherins; D050796:STAT3 Transcription Factor; C494088:Stat3 protein, rat; D014157:Transcription Factors; D004398:Dyneins