title: An effective method of RNA isolation from Fallopia multiflora tuberous roots.
abstract: To isolate high-quality total RNA from Fallopia multiflora tuberous roots is difficult because of the presence of high levels of carbohydrates, phenolics, and other secondary metabolites. Since several procedures specialized for RNA isolation from polysaccharides and phenols rich tissues have resulted in poor yields, in this study, we developed a modified protocol that was derived from the traditional CTAB method. The protocol was able to produce high-quality and intact RNA from the tuberous roots of F. multiflora. The yield of total RNA was more than 0.15 mg/g fresh weight, with an A260/A280 ratio of 1.9-2.0. The obtained RNA was of sufficient quality and suitable for downstream application such as reverse-transcription polymerase chain reaction (RT-PCR), Northern hybridization, and cDNA library construction. The protocol may also have wider applicability for total RNA isolation from other plant species with tuberous roots.
authors.affiliation: Guangdong Provincial Key Lab of Biotechnology for Plant Development, College of Life Sciences, South China Normal University, Guangzhou, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.forename: Shu-Jing
authors.initials: SJ
authors.lastname: Sheng
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Tan
authors.forename: Yan-Jing
authors.initials: YJ
authors.lastname: Shen
authors.forename: Hong-Qing
authors.initials: HQ
authors.lastname: Li
authors.forename: Shu-Jin
authors.initials: SJ
authors.lastname: Zhao
mesh_terms: D015152:Blotting, Northern; D004587:Electrophoresis, Agar Gel; D015723:Gene Library; D010936:Plant Extracts / Q000302:isolation & purification / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry*; D019664:Polygonaceae / Q000737:chemistry* / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D010936:Plant Extracts; D018749:RNA, Plant