title: [Protective effects of Leonurus japonicas on myocardial remodeling induced by isoproterenol in rats].
abstract: OBJECTIVE
  To investigate the effects of Leonurus japonicas on myocardial remodeling induced by isoproterenol (ISO) in rats.
  
  
  METHODS
  The model of rat myocardial remodeling was established by subcutaneous injection of ISO (20,10 and 5 mg/kg for 3 days, subsequently 3 mg/kg for 7 days). Rats were randomly divided into five groups: Control, ISO, ISO + enalapril, ISO + Leonurus 8 g/ kg and ISO + Leonurus 16 g/kg. Recorded the values of LVSP, LVEDP, +/- dp/dt (max), cardiac output ( CO). Weighed the body weight (BW), heart weight (HW) and left ventricular weight (LVW) of rats to calculate the values of HW/BW and LVW/BW. The hydroxyproline contents were investigated by spectrophotometric measurement. The contents of type I collagen and type III collagen and ratio of I/III collagen were assessed by immunohistochemical stain.
  
  
  RESULTS
  Leonurus (16 g/kg x day(-1)) could increased the value of LVSP, + dp/dt(max), CO (P < 0.05). Leonurus (8 g/kg) could increased the value of -dp/dt(max), decreased the ratios of HW/BW and LVW/BW (P < 0.05), the contents of hydroxyproline, type I collagen, type III collagen and the ratios of I/III collagen (P < 0.05).
  
  
  CONCLUSIONS
  Leonurus at a dosage of 16 g/kg may improve the systolic function; Leonurus at a dosage of 8 g/kg may improve the diastolic function, down-regulate the expression of collagen and normalize the ratio of I/III collagen.
authors.affiliation: Department of Pathology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. pathofshutcm@yahoo.com.cn
authors.forename: Chen
authors.initials: C
authors.lastname: Zhang
authors.forename: Yan-Pin
authors.initials: YP
authors.lastname: Gu
authors.forename: Bao-Ping
authors.initials: BP
authors.lastname: Yuan
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Guo
authors.forename: Hong-Chang
authors.initials: HC
authors.lastname: Wei
authors.forename: Rong
authors.initials: R
authors.lastname: Lv
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology; D003094:Collagen / Q000378:metabolism; D004195:Disease Models, Animal; D006439:Hemodynamics / Q000187:drug effects; D006909:Hydroxyproline / Q000378:metabolism; D007150:Immunohistochemistry; D007545:Isoproterenol / Q000008:administration & dosage; D031332:Leonurus / Q000737:chemistry*; D008297:Male; D009206:Myocardium / Q000378:metabolism / Q000473:pathology*; D009929:Organ Size / Q000187:drug effects; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D016277:Ventricular Function, Left / Q000187:drug effects*; D017725:Ventricular Pressure / Q000187:drug effects; D020257:Ventricular Remodeling / Q000187:drug effects*
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D003094:Collagen; D007545:Isoproterenol; D006909:Hydroxyproline