title: Antibodies against the tom40 subunit of the translocase of the outer mitochondrial membrane complex and cognitive impairment in Alzheimer's disease.
abstract: Recent studies suggest that microvascular abnormalities are involved in pathology and progression of Alzheimer's disease. The purpose of this study was to examine the presence of antibodies against cerebral microvascular endothelial cells specific for Alzheimer's disease, and to evaluate the association of these antibodies with cognitive impairment. The study included patients with Alzheimer's disease (age ≥60 years; 24 patients), control subjects without neurological diseases (age ≥60 years; 19 subjects), patients with multiple sclerosis (all ages; 17 patients), and healthy control subjects (age <40 years; 18 subjects). Serum was analyzed with 2-dimensional electrophoresis and Western blot, with cultured human brain microvascular endothelial cells as the antigen source. The anti-Tom40 antibody was identified significantly more frequently in patients with Alzheimer's disease than control subjects or patients with multiple sclerosis. In patients with Alzheimer's disease, the mean scores for the Mini-Mental State Examination were significantly lower for patients who were positive for anti-Tom40 antibody than those who were negative for anti-Tom40 antibody. In summary, the anti-Tom40 antibody is significantly associated with cognitive impairment in patients with Alzheimer's disease.
authors.affiliation: Departments of Neurology and Geriatrics, Gifu University Graduate School of Medicine, Gifu City, Japan. kimura1@gifu-u.ac.jp
authors.forename: Akio
authors.initials: A
authors.lastname: Kimura
authors.forename: Takeo
authors.initials: T
authors.lastname: Sakurai
authors.forename: Megumi
authors.initials: M
authors.lastname: Yamada
authors.forename: Akihiro
authors.initials: A
authors.lastname: Koumura
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Hayashi
authors.forename: Yuji
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Isao
authors.initials: I
authors.lastname: Hozumi
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Ohtaki
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Chousa
authors.forename: Masao
authors.initials: M
authors.lastname: Takemura
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Seishima
authors.forename: Takashi
authors.initials: T
authors.lastname: Inuzuka
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000150:complications; D000906:Antibodies / Q000378:metabolism*; D001921:Brain / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure*; D003072:Cognition Disorders / Q000209:etiology / Q000473:pathology*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D051336:Mitochondrial Membranes / Q000378:metabolism* / Q000473:pathology; D024101:Mitochondrial Proteins / Q000276:immunology* / Q000378:metabolism; D009483:Neuropsychological Tests; D021381:Protein Transport; D011569:Psychiatric Status Rating Scales; D055815:Young Adult
chemical_list: D000906:Antibodies; D024101:Mitochondrial Proteins