title: Mechanical properties of tyramine substituted-hyaluronan enriched fascia extracellular matrix.
abstract: Naturally occurring biomaterial scaffolds derived from extracellular matrix (ECM) have been the topic of recent investigation in the context of rotator cuff tendon repair. We previously reported a method to treat fascia ECM with high molecular weight tyramine substituted-hyaluronan (TS-HA) for use as a tendon augmentation scaffold. The presence of crosslinked TS-HA in fascia was associated with an increased macrophage and giant cell response compared to water-treated controls after implantation in a rat abdominal wall model. The objective of this study was to determine the extent to which TS-HA treatment was associated with mechanical property changes of fascia after implantation in the rat model. Fascia samples in all groups demonstrated time-dependent decreases in mechanical properties. TS-HA-treated fascia with crosslinking exhibited a lower toe modulus, a trend toward lower toe stiffness, and a higher transition strain than water-treated controls not only after implantation, but also at time zero. TS-HA treatment, with or without crosslinking, had no significant effect on time-zero or post-implantation load relaxation ratio, load relaxation rate, linear-region stiffness, or linear-region modulus. Our findings demonstrated that the particular TS-HA treatment employed in this study decreased the low-load elastic mechanical properties of fascia ECM, in keeping with the heightened macrophage and giant cell host response seen previously. This work provides a starting point and guidance for investigating alternative HA treatment strategies.
authors.affiliation: Department of Biomedical Engineering, Lerner Research Institute and Orthopedic Research Center, Cleveland Clinic, Cleveland, Ohio, USA.
authors.forename: Likang
authors.initials: L
authors.lastname: Chin
authors.forename: Anthony
authors.initials: A
authors.lastname: Calabro
authors.forename: Esteban
authors.initials: E
authors.lastname: Walker
authors.forename: Kathleen A
authors.initials: KA
authors.lastname: Derwin
mesh_terms: D034861:Abdominal Wall / Q000473:pathology / Q000601:surgery; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D005109:Extracellular Matrix / Q000737:chemistry*; D005205:Fascia / Q000033:anatomy & histology / Q000737:chemistry*; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D008297:Male; D008422:Materials Testing; D051381:Rats; D011917:Rats, Inbred Lew; D013314:Stress, Mechanical; D054457:Tissue Scaffolds / Q000737:chemistry; D014439:Tyramine / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D006820:Hyaluronic Acid; D014439:Tyramine