title: Effects of dietary glutamine supplementation on the body composition and protein status of early-weaned mice inoculated with Mycobacterium bovis Bacillus Calmette-Guerin.
abstract: Glutamine, one of the most abundant amino acids found in maternal milk, favors protein anabolism. Early-weaned babies are deprived of this source of glutamine, in a period during which endogenous biosynthesis may be insufficient for tissue needs in states of metabolic stress, mainly during infections. The objective of this study was to verify the effects of dietary glutamine supplementation on the body composition and visceral protein status of early-weaned mice inoculated with Mycobacterium bovis Bacillus Calmette-Guérin (BCG). Mice were weaned early on their 14th day of life and seperated into two groups, one of which was fed a glutamine-free diet (n = 16) and the other a glutamine-supplemented diet (40 g/kg diet) (n = 16). At 21 days of age, some mice were intraperitoneally injected with BCG. Euthanasia was performed at the 28th day of age. BCG inoculation significantly reduced body weight (P < 0.001), lean mass (P = 0.002), water (P = 0.006), protein (P = 0.007) and lipid content (P = 0.001) in the carcass. Dietary glutamine supplementation resulted in a significant increase in serum IGF-1 (P = 0.019) and albumin (P = 0.025) concentration, muscle protein concentration (P = 0.035) and lipid content (P = 0.002) in the carcass. In conclusion, dietary glutamine supplementation had a positive influence on visceral protein status but did not affect body composition in early-weaned mice inoculated with BCG.
authors.affiliation: Department of Nutrition, School of Public Health, University of Sao Paulo, Brazil. mcarolborges@usp.br
authors.forename: Marcelo Macedo
authors.initials: MM
authors.lastname: Rogero
authors.forename: Maria Carolina
authors.initials: MC
authors.lastname: Borges
authors.forename: Inar Alves
authors.initials: IA
authors.lastname: de Castro
authors.forename: Ivanir S O
authors.initials: IS
authors.lastname: Pires
authors.forename: Primavera
authors.initials: P
authors.lastname: Borelli
authors.forename: Julio
authors.initials: J
authors.lastname: Tirapegui
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D000831:Animals, Newborn; D001500:BCG Vaccine / Q000008:administration & dosage; D001823:Body Composition / Q000187:drug effects*; D019587:Dietary Supplements; D005973:Glutamine / Q000494:pharmacology*; D008297:Male; D051379:Mice; D011506:Proteins / Q000187:drug effects*; D014886:Weaning
chemical_list: D000276:Adjuvants, Immunologic; D001500:BCG Vaccine; D011506:Proteins; D005973:Glutamine