title: Pre-power-stroke cross-bridges contribute to force transients during imposed shortening in isolated muscle fibers.
abstract: When skeletal muscles are activated and mechanically shortened, the force that is produced by the muscle fibers decreases in two phases, marked by two changes in slope (P₁ and P₂) that happen at specific lengths (L₁ and L₂). We tested the hypothesis that these force transients are determined by the amount of myosin cross-bridges attached to actin and by changes in cross-bridge strain due to a changing fraction of cross-bridges in the pre-power-stroke state. Three separate experiments were performed, using skinned muscle fibers that were isolated and subsequently (i) activated at different Ca²⁺ concentrations (pCa²⁺ 4.5, 5.0, 5.5, 6.0) (n = 13), (ii) activated in the presence of blebbistatin (n = 16), and (iii) activated in the presence of blebbistatin at varying velocities (n = 5). In all experiments, a ramp shortening was imposed (amplitude 10%L₀, velocity 1 L₀•sarcomere length (SL)•s⁻¹), from an initial SL of 2.5 µm (except by the third group, in which velocities ranged from 0.125 to 2.0 L₀•s⁻¹). The values of P₁, P₂, L₁, and L₂ did not change with Ca²⁺ concentrations. Blebbistatin decreased P₁, and it did not alter P₂, L₁, and L₂. We developed a mathematical cross-bridge model comprising a load-dependent power-stroke transition and a pre-power-stroke cross-bridge state. The P₁ and P₂ critical points as well as the critical lengths L₁ and L₂ were explained qualitatively by the model, and the effects of blebbistatin inhibition on P₁ were also predicted. Furthermore, the results of the model suggest that the mechanism by which blebbistatin inhibits force is by interfering with the closing of the myosin upper binding cleft, biasing cross-bridges into a pre-power-stroke state.
authors.affiliation: Department of Kinesiology and Physical Education, McGill University, Montreal, Quebec, Canada.
authors.forename: Fabio C
authors.initials: FC
authors.lastname: Minozzo
authors.forename: Lennart
authors.initials: L
authors.lastname: Hilbert
authors.forename: Dilson E
authors.initials: DE
authors.lastname: Rassier
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D003198:Computer Simulation; D006576:Heterocyclic Compounds, 4 or More Rings / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D066298:In Vitro Techniques; D008954:Models, Biological; D009119:Muscle Contraction / Q000187:drug effects*; D018485:Muscle Fibers, Skeletal / Q000187:drug effects* / Q000502:physiology*; D011817:Rabbits; D012518:Sarcomeres / Q000502:physiology*
chemical_list: D006576:Heterocyclic Compounds, 4 or More Rings; C472645:blebbistatin; D002118:Calcium