title: Comparison of interferon-γ-, interleukin (IL)-17- and IL-22-expressing CD4 T cells, IL-22-expressing granulocytes and proinflammatory cytokines during latent and active tuberculosis infection.
abstract: In this study, we investigated the role and expression of T helper type 17 (Th17) cells and Th17 cytokines in human tuberculosis. We show that the basal proportion of interferon (IFN)-γ-, interleukin (IL)-17- and IL-22-expressing CD4(+)  T cells and IL-22-expressing granulocytes in peripheral blood were significantly lower in latently infected healthy individuals and active tuberculosis patients compared to healthy controls. In contrast, CD4(+)  T cells expressing IL-17, IL-22 and IFN-γ were increased significantly following mycobacterial antigens stimulation in both latent and actively infected patients. Interestingly, proinflammatory IFN-γ and tumour necrosis factor (TNF)-α were increased following antigen stimulation in latent infection. Similarly, IL-1β, IL-4, IL-8, IL-22 and TNF-α were increased in the serum of latently infected individuals, whereas IL-6 and TNF-α were increased significantly in actively infected patients. Overall, we observed differential induction of IL-17-, IL-22- and IFN-γ-expressing CD4(+)  T cells, IL-22-expressing granulocytes and proinflammatory cytokines in circulation and following antigenic stimulation in latent and active tuberculosis.
authors.affiliation: Ottawa Hospital Research Institute, Ottawa, ON, Canada.
authors.forename: J
authors.initials: J
authors.lastname: Cowan
authors.forename: S
authors.initials: S
authors.lastname: Pandey
authors.forename: L G
authors.initials: LG
authors.lastname: Filion
authors.forename: J B
authors.initials: JB
authors.lastname: Angel
authors.forename: A
authors.initials: A
authors.lastname: Kumar
authors.forename: D W
authors.initials: DW
authors.lastname: Cameron
mesh_terms: D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism*; D016207:Cytokines / Q000097:blood*; D005006:Ethnicity; D005260:Female; D006098:Granulocytes / Q000378:metabolism*; D006801:Humans; D007249:Inflammation / Q000097:blood; D007371:Interferon-gamma / Q000097:blood*; D007378:Interleukins / Q000097:blood*; D055985:Latent Tuberculosis / Q000097:blood / Q000208:ethnology / Q000276:immunology; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000097:blood; D020133:Reverse Transcriptase Polymerase Chain Reaction; D058504:Th17 Cells / Q000378:metabolism; D014376:Tuberculosis / Q000097:blood* / Q000208:ethnology / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D055815:Young Adult; D000097043:Interleukin-22
chemical_list: D016207:Cytokines; D007371:Interferon-gamma; D007378:Interleukins; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D000097043:Interleukin-22