title: Integrating sphere setup for the traceable measurement of absolute photoluminescence quantum yields in the near infrared.
abstract: There is an increasing interest in chromophores absorbing and emitting in the near-infrared (NIR) spectral region, e.g., for applications as fluorescent reporters for optical imaging techniques and hence, in reliable methods for the characterization of their signal-relevant properties like the fluorescence quantum yield (Φ(f)) and brightness. The lack of well established Φ(f) standards for the NIR region in conjunction with the need for accurate Φ(f) measurements in transparent and scattering media encouraged us to built up an integrating sphere setup for spectrally resolved measurements of absolute fluorescence traceable to radiometric scales. Here, we present the design of this setup and its characterization and validation including an uncertainty budget for the determination of absolute Φ(f) in the visible and NIR. To provide the basis for better measurements of Φ(f) in the spectral window from ca. 600 to 1000 nm used, e.g., for optical imaging, the absolute Φ(f) of a set of NIR chromophores covering this spectral region are measured and compared to relative values obtained using rhodamine 101 as Φ(f) standard. Additionally, the absolute Φ(f) values of some red dyes that are among the most commonly used labels in the life sciences are presented as well as the absolute quantum yield of an optical probe for tumor imaging.
authors.affiliation: BAM Federal Institute for Materials Research and Testing, Richard-Willstaetter-Strasse 11, 12489 Berlin, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Würth
authors.forename: Jutta
authors.initials: J
authors.lastname: Pauli
authors.forename: Cornelia
authors.initials: C
authors.lastname: Lochmann
authors.forename: Monika
authors.initials: M
authors.lastname: Spieles
authors.forename: Ute
authors.initials: U
authors.lastname: Resch-Genger
mesh_terms: D004396:Coloring Agents / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D009369:Neoplasms / Q000473:pathology; D011789:Quantum Theory; D012235:Rhodamines / Q000737:chemistry; D019265:Spectroscopy, Near-Infrared
chemical_list: D004396:Coloring Agents; D005456:Fluorescent Dyes; D012235:Rhodamines; C049025:rhodamine 110