title: Preservation of mucus in histological sections, immunostaining of mucins in fixed tissue, and localization of bacteria with FISH.
abstract: As mucus is highly hydrated, special care has to be taken to preserve this in histological preparations during immunostaining. Here, we describe how to fix tissues in such a way that the mucus is preserved in paraffin-embedded tissue. We also describe how the major macromolecular components in the mucus, the mucins, are immunostained and how bacteria can be localized in preparations with preserved mucus by fluorescent in situ hybridization.
authors.affiliation: Department of Medical Biochemistry, Mucin Biology Group, University of Gothenburg, Gothenburg, Sweden.
authors.forename: Malin E V
authors.initials: ME
authors.lastname: Johansson
authors.forename: Gunnar C
authors.initials: GC
authors.lastname: Hansson
mesh_terms: D000818:Animals; D001419:Bacteria / Q000737:chemistry / Q000302:isolation & purification*; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D009077:Mucins / Q000032:analysis* / Q000737:chemistry*; D009093:Mucus / Q000737:chemistry*; D016707:Tissue Fixation
chemical_list: D009077:Mucins