title: Development of a hepatitis delta virus antibody assay for study of the prevalence of HDV among individuals infected with hepatitis B virus in China.
abstract: Co-infection with hepatitis delta virus (HDV) and hepatitis B virus (HBV) has been shown to be associated with a more severe form of acute and chronic hepatitis. Cloning and expression of recombinant HDV antigen (rHDAg) in Escherichiacoli are described. Using purified rHDAg, a cost-effective indirect anti-HDV enzyme-linked immunosorbent assay (ELISA) kit was developed. Direct comparison of 15 known HDV-positive sera and 15 HDV-negative sera showed concordance agreement between the new assay kit and the Abbott Murex Anti-Delta (total) kit. In addition, 1,486 hepatitis B surface antigen (HBsAg) positive blood samples collected from various areas of China were tested using this indirect anti-HDV ELISA. It was found that 1.2% (95% CI: 0.7-1.9%) of the samples were anti-HDAg positive. It is suggested that the prevalence of HDV and HBV co-infection in China is relatively low.
authors.affiliation: State Key Laboratory for Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping District, Beijing, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Shen
authors.forename: Ying
authors.initials: Y
authors.lastname: Gu
authors.forename: Le
authors.initials: L
authors.lastname: Sun
authors.forename: Yingchao
authors.initials: Y
authors.lastname: Yang
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Yimin
authors.initials: Y
authors.lastname: Li
authors.forename: Shengli
authors.initials: S
authors.lastname: Bi
mesh_terms: D002681:China / Q000453:epidemiology; D060085:Coinfection; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006508:Hepatitis Antibodies / Q000276:immunology*; D006509:Hepatitis B / Q000150:complications* / Q000821:virology; D006515:Hepatitis B virus / Q000276:immunology; D003699:Hepatitis D / Q000150:complications / Q000453:epidemiology*; D003698:Hepatitis Delta Virus / Q000235:genetics / Q000276:immunology*; D035923:Hepatitis delta Antigens / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D015995:Prevalence
chemical_list: D006508:Hepatitis Antibodies; D035923:Hepatitis delta Antigens