title: Inhibitory effects of terpenoids from the fermented broth of the ascomycete Stilbohypoxylon elaeicola YMJ173 on nitric oxide production in RAW264.7 macrophages.
abstract: A series of six isopimarane-type diterpene glycosides, along with an eremophilane-type sesquiterpene, i.e., elaeicolasides A-C (1-3, resp.), 16-(α-D-mannopyranosyloxy)isopimar-7-en-19-oic acid (4), hymatoxin K (5), hymatoxin L (6), and elaeicolalactone (7), were isolated from the AcOEt extract of the fermented broth of Stilbohypoxylon elaeicola YMJ173. Among these, 1-3 and 7 are new compounds based on their spectroscopic data and sugar composition analysis. The effects of 1-7 on the inhibition of nitric oxide (NO) production in lipopolysaccharide (LPS)-activated murine macrophage RAW264.7 cells were evaluated. All these compounds inhibited NO production, detected as nitrite in the culture medium, in activated macrophages without any cytotoxicity at a concentration of 100 μM. Among these compounds, 2 showed a significant activity with the average maximum inhibition (E(max)) and median inhibitory concentration (IC(50)) values of 93.3±0.5% and 79.3±0.4 μM, respectively.
authors.affiliation: L5 Research Center, China Medical University Hospital, Taichung 404, Taiwan.
authors.forename: Guei-Jane
authors.initials: GJ
authors.lastname: Wang
authors.forename: Wen-Li
authors.initials: WL
authors.lastname: Liang
authors.forename: Yu-Ming
authors.initials: YM
authors.lastname: Ju
authors.forename: Wen-Bin
authors.initials: WB
authors.lastname: Yang
authors.forename: Ya-Wen
authors.initials: YW
authors.lastname: Chang
authors.forename: Tzong-Huei
authors.initials: TH
authors.lastname: Lee
mesh_terms: D000818:Animals; D002460:Cell Line; D005285:Fermentation; D008070:Lipopolysaccharides / Q000633:toxicity; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008968:Molecular Conformation; D009569:Nitric Oxide / Q000378:metabolism*; D013729:Terpenes / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D013098:Xylariales / Q000254:growth & development / Q000378:metabolism*
chemical_list: D008070:Lipopolysaccharides; D013729:Terpenes; D009569:Nitric Oxide