title: Fin reduction is a novel and unexpected teratogenic effect of amikacin-treated zebrafish embryos.
abstract: We used zebrafish as a model to assess amikacin-induced embryotoxicity. We exposed zebrafish embryos to amikacin, using different amikacin doses (0-10 ppm), durations (12-48 h), and onsets (0, 24, 48 hpf). Amikacin-induced embryonic toxicity and reduced survival rate were found dependent on the exposure dose, duration and onset. Based on immunostaining with neuron-specific antibodies, amikacin reduced the number and size of zebrafish neuromasts. In addition, Amikacin caused pelvic, dorsal and anal fin defects in dose-dependent and duration-dependent manners. Proliferating cell nuclear antigen immunostaining revealed that amikacin-induced fin defects were not due to reduction of proliferating mesenchymal cells. TUNEL assay demonstrated that amikacin-induced fin defects might not associate with apoptosis. Therefore, further investigations are required to elucidate if other cell death pathways are involved in amikacin-induced fin defects.
authors.affiliation: Institute of Medical Sciences, Buddhist Tzu Chi University, Hualien, Taiwan.
authors.forename: Ying-Hsin
authors.initials: YH
authors.lastname: Chen
authors.forename: I-Ting
authors.initials: IT
authors.lastname: Tsai
authors.forename: Chi-Chung
authors.initials: CC
authors.lastname: Wen
authors.forename: Yun-Hsin
authors.initials: YH
authors.lastname: Wang
authors.forename: Chien-Chung
authors.initials: CC
authors.lastname: Cheng
authors.forename: Sheng-Chuan
authors.initials: SC
authors.lastname: Hu
authors.forename: Yau-Hung
authors.initials: YH
authors.lastname: Chen
mesh_terms: D000014:Abnormalities, Drug-Induced / Q000209:etiology*; D000583:Amikacin / Q000633:toxicity*; D058500:Animal Fins / Q000002:abnormalities*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004625:Embryo, Nonmammalian / Q000187:drug effects; D015027:Zebrafish
chemical_list: D000900:Anti-Bacterial Agents; D000583:Amikacin