title: Protective effects of a Rhodiola crenulata extract and salidroside on hippocampal neurogenesis against streptozotocin-induced neural injury in the rat.
abstract: Previously we have demonstrated that a Rhodiola crenulata extract (RCE), containing a potent antioxidant salidroside, promotes neurogenesis in the hippocampus of depressive rats. The current study was designed to further investigate the protective effect of the RCE on neurogenesis in a rat model of Alzheimer's disease (AD) induced by an intracerebroventricular injection of streptozotocin (STZ), and to determine whether this neuroprotective effect is induced by the antioxidative activity of salidroside. Our results showed that pretreatment with the RCE significantly improved the impaired neurogenesis and simultaneously reduced the oxidative stress in the hippocampus of AD rats. In vitro studies revealed that (1) exposure of neural stem cells (NSCs) from the hippocampus to STZ strikingly increased intracellular reactive oxygen species (ROS) levels, induced cell death and perturbed cell proliferation and differentiation, (2) hydrogen peroxide induced similar cellular activities as STZ, (3) pre-incubation of STZ-treated NSCs with catalase, an antioxidant, suppressed all these cellular activities induced by STZ, and (4) likewise, pre-incubation of STZ-treated NSCs with salidroside, also an antioxidant, suppressed all these activities as catalase: reduction of ROS levels and NSC death with simultaneous increases in proliferation and differentiation. Our findings indicated that the RCE improved the impaired hippocampal neurogenesis in the rat model of AD through protecting NSCs by its main ingredient salidroside which scavenged intracellular ROS.
authors.affiliation: Division of Neuroscience, Department of Histology and Embryology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China.
authors.forename: Ze-qiang
authors.initials: ZQ
authors.lastname: Qu
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Yuan-shan
authors.initials: YS
authors.lastname: Zeng
authors.forename: Yu-kun
authors.initials: YK
authors.lastname: Lin
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Zhi-qiang
authors.initials: ZQ
authors.lastname: Zhong
authors.forename: Wood Yee
authors.initials: WY
authors.lastname: Chan
mesh_terms: D000818:Animals; D002374:Catalase / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D016166:Free Radical Scavengers / Q000494:pharmacology; D005960:Glucosides / Q000494:pharmacology*; D006624:Hippocampus / Q000187:drug effects* / Q000293:injuries* / Q000378:metabolism / Q000473:pathology; D007267:Injections; D008297:Male; D058953:Neural Stem Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D055495:Neurogenesis / Q000187:drug effects*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D018696:Neuroprotective Agents / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects; D010636:Phenols / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D029771:Rhodiola / Q000737:chemistry*; D013311:Streptozocin
chemical_list: D016166:Free Radical Scavengers; D005960:Glucosides; D018696:Neuroprotective Agents; D010636:Phenols; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D013311:Streptozocin; D002374:Catalase; C009172:rhodioloside