title: Comparative analysis of expression of human telomerase catalytic subunit at the transcription level in cell cultures of different origin.
abstract: The expression of human telomerase catalytic subunit in HL-60 and HT-1080 malignant transformed cells and telomerized fibroblasts was studied by quantitative PCR. It was found that the number of transcripts of human telomerase catalytic subunit per cell in telomerized fibroblasts could be hundreds of times higher than in HL-60 and HT-1080 cells. Telomerized fibroblast cultures are suggested as experimental systems for selection of basal compounds for creation of anticancer drug prototypes, the molecular target of which is human telomerase catalytic subunit. The effects of human telomerase catalytic subunit expression on the fibroblast proteome are analyzed.
authors.affiliation: V. N. Orekhovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow, Russia. cheglakovib@yahoo.ca
authors.forename: I B
authors.initials: IB
authors.lastname: Cheglakov
authors.forename: S P
authors.initials: SP
authors.lastname: Radko
authors.forename: K N
authors.initials: KN
authors.lastname: Yarygin
authors.forename: Kh S
authors.initials: KhS
authors.lastname: Vishniakova
authors.forename: E E
authors.initials: EE
authors.lastname: Egorov
mesh_terms: D045744:Cell Line, Tumor; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D006801:Humans; D019098:Telomerase / Q000235:genetics* / Q000378:metabolism*; D016615:Telomere / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: C509186:TERT protein, human; D019098:Telomerase