title: Predominance of large VLDL particles in metabolic syndrome, detected by size exclusion liquid chromatography.
abstract: OBJECTIVE
  To study size heterogeneity of triglyceride rich lipoproteins (TRL) in metabolic syndrome (MS).
  
  
  METHODS
  Thirty MS patients and 14 healthy subjects were included. In fasting serum we measured: lipid profile, free fatty acids (FFA) and adiponectin; TRL were isolated (d<1.006 g/mL) and analysis by size exclusion HPLC followed by UV detection was performed; each subfraction was expressed as percentage of total TRL.
  
  
  RESULTS
  MS patients, even those with normal triglycerides, presented higher proportion of very large VLDL (90 nm diameter) and large VLDL (60 nm) and slightly lower of typical VLDL (37 nm) (p<0.04); increased FFA (p=0.04) and lower adiponectin (p=0.001). FFA correlated with large VLDL% (r=0.58; p=0.003), independently of insulin-resistance and waist. Furthermore, the lower the adiponectin, the greater the predominance of large VLDL (r=-0.40; p=0.04).
  
  
  CONCLUSIONS
  MS was associated with large VLDL, described as more atherogenic beyond triglyceride levels. Size exclusion HPLC would represent a useful tool for assessing subfractions' lipoprotein profile.
authors.affiliation: Laboratory of Lipids and Lipoproteins, Department of Clinical Biochemistry, Faculty of Pharmacy and Biochemistry, INFIBIOC, University of Buenos Aires, Argentina.
authors.forename: Diego
authors.initials: D
authors.lastname: Lucero
authors.forename: Valeria
authors.initials: V
authors.lastname: Zago
authors.forename: Gustavo H
authors.initials: GH
authors.lastname: López
authors.forename: Leonardo
authors.initials: L
authors.lastname: Cacciagiú
authors.forename: Graciela I
authors.initials: GI
authors.lastname: López
authors.forename: Regina
authors.initials: R
authors.lastname: Wikinski
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Nakajima
authors.forename: Laura
authors.initials: L
authors.lastname: Schreier
mesh_terms: D052242:Adiponectin / Q000097:blood; D000328:Adult; D001118:Argentina / Q000453:epidemiology; D050197:Atherosclerosis / Q000453:epidemiology; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D006801:Humans; D008079:Lipoproteins, VLDL / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification; D008297:Male; D024821:Metabolic Syndrome / Q000097:blood*; D008875:Middle Aged; D010316:Particle Size; D012306:Risk; D014280:Triglycerides / Q000097:blood; D014461:Ultracentrifugation
chemical_list: C499918:ADIPOQ protein, human; D052242:Adiponectin; D005230:Fatty Acids, Nonesterified; D008079:Lipoproteins, VLDL; D014280:Triglycerides; C030106:very low density lipoprotein triglyceride