title: Conformational properties of two exopolysaccharides produced by Inquilinus limosus, a cystic fibrosis lung pathogen.
abstract: Inquilinus limosus is a multi-resistant bacterium found in the respiratory tract of patients with cystic fibrosis. This bacterium produces two unique fully pyruvylated exopolysaccharides in similar quantities: an α-(1→2)-linked mannan and a β-(1→3)-linked glucan. We employed molecular modelling methods to probe the characteristic conformations and dynamics of these polysaccharides, with corroboration from potentiometric titrations and circular dichroism experiments. Our calculations reveal different structural motifs for the mannan and glucan polysaccharides: the glucan forms primarily right-handed helices with a wide range of extensions, while the mannan forms only left-handed helices. This finding is supported by our circular dichroism experiments. Our calculations also show that the (1→3)-β-d-Glcp linkage is more dynamically flexible than the (1→2)-α-d-Manp: the glucan characteristically forms a range of wide helices with large central cavities. In contrast, the mannan forms rigid regular 'bottlebrush' helices with a minimal central cavity. The widely different character of these two polymers suggests a possible differentiation of biological roles.
authors.affiliation: Department of Computer Science, University of Cape Town, Rondebosch, South Africa. mkuttel@cs.uct.ac.za
authors.forename: Michelle
authors.initials: M
authors.lastname: Kuttel
authors.forename: Neil
authors.initials: N
authors.lastname: Ravenscroft
authors.forename: Michela
authors.initials: M
authors.lastname: Foschiatti
authors.forename: Paola
authors.initials: P
authors.lastname: Cescutti
authors.forename: Roberto
authors.initials: R
authors.lastname: Rizzo
mesh_terms: D002236:Carbohydrate Conformation; D055598:Chemical Phenomena; D003550:Cystic Fibrosis / Q000382:microbiology*; D005936:Glucans / Q000737:chemistry; D008168:Lung / Q000382:microbiology*; D008351:Mannans / Q000737:chemistry; D056004:Molecular Dynamics Simulation; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis* / Q000737:chemistry*; D012244:Rhodospirillaceae / Q000737:chemistry* / Q000378:metabolism* / Q000472:pathogenicity
chemical_list: D005936:Glucans; D008351:Mannans; D011135:Polysaccharides, Bacterial