title: The impact of Porphyromonas gingivalis lipids on apoptosis of primary human chondrocytes.
abstract: The role of oral bacterial infections including periodontal disease in the pathogenesis of rheumatoid arthritis (RA) has gained increasing interest. Among the major periodontal pathogens, Porphyromonas gingivalis has been mostly associated with RA pathogenesis. The aim of this study was to analyze the effect of P. gingivalis total lipid (TL) fraction and dihydroceramides, as potent virulence factors, on human primary chondrocytes. Primary chondrocyte cultures were incubated with P. gingivalis phosphoglycerol dihydroceramide (PG DHC) lipids, the TL fraction or phosphoethanolamine dihydroceramide. Cell morphology changes were determined by phase contrast light microscopy. Early and late apoptosis cell analysis was performed by Annexin-V, active caspases, and 7-Aminoactinomycin D staining, and examined by flow cytometry, and cell necrosis was evaluated by lactate dehydrogenase release. Procaspase-3 activation was determined by Western blot analysis. Microscopic analysis showed altered cell morphology and cell shrinkage following incubation with P. gingivalis TLs and PG DHC lipids. Flow cytometry demonstrated an increase of Annexin-V positive and active caspases positive chondrocytes after incubation with TL and PG DHC fractions but not after phosphoethanolamine dihydroceramide (control lipid) treatment or in untreated control cells. Furthermore, Western blot analysis showed an early cleavage of procaspase-3 after 1 hr. Significant lactate dehydrogenase release following incubation with P. gingivalis lipids was demonstrated. The present data demonstrate that P. gingivalis lipids promote apoptosis in primary human chondrocytes, and thereby may contribute to the joint damage seen in the pathogenesis of RA.
authors.affiliation: Department of Traumatology and Orthopaedics, Charité-Universitätsmedizin, Berlin, Germany. eric.roehner@charite.de
authors.forename: Eric
authors.initials: E
authors.lastname: Röhner
authors.forename: Paula
authors.initials: P
authors.lastname: Hoff
authors.forename: Georg
authors.initials: G
authors.lastname: Matziolis
authors.forename: Carsten
authors.initials: C
authors.lastname: Perka
authors.forename: Birgit
authors.initials: B
authors.lastname: Riep
authors.forename: Frank C
authors.initials: FC
authors.lastname: Nichols
authors.forename: Andrej M
authors.initials: AM
authors.lastname: Kielbassa
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Detert
authors.forename: Gerd R
authors.initials: GR
authors.lastname: Burmester
authors.forename: Frank
authors.initials: F
authors.lastname: Buttgereit
authors.forename: Janine
authors.initials: J
authors.lastname: Zahlten
authors.forename: Nicole
authors.initials: N
authors.lastname: Pischon
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology*; D005260:Female; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008055:Lipids / Q000494:pharmacology*; D008297:Male; D008875:Middle Aged; D016966:Porphyromonas gingivalis / Q000737:chemistry*
chemical_list: D008055:Lipids; D007770:L-Lactate Dehydrogenase; D053148:Caspase 3