title: Novel application of Ag nanoclusters in fluorescent imaging of human serum proteins after native polyacrylamide gel electrophoresis (PAGE).
abstract: We have developed a novel application for DNA oligonucleotide-stabilized Ag nanoclusters in fluorescent imaging of human serum proteins after native polyacrylamide gel electrophoresis (PAGE). Oligonucleotide-stabilized Ag nanoclusters were used as fluorescent probes for direct detection of proteins after native PAGE. Some relatively low-abundance proteins, such as α-1-antichymotrypsin (ACT) and α-2-glycoprotein 1, zinc (ZAG) were easily detected by oligonucleotide-stabilized Ag nanocluster-based fluorescent imaging and identified by MS and MS/MS techniques, without the need of expensive antibodies or tedious immunoassay procedures. The pH condition for the oligonucleotide-stabilized Ag nanocluster solution was optimized and the possible mechanism of interaction between proteins and DNA oligonucleotide-stabilized Ag nanoclusters was analyzed. As a novel fluorescent detection method it is simple, fast, nontoxic and sensitive, and it shows great analytical potential in proteome research and in biochemistry.
authors.affiliation: College of Chemistry, Beijing Normal University, Beijing 100875, P.R. China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Lingyun
authors.initials: L
authors.lastname: Huang
authors.forename: Dacheng
authors.initials: D
authors.lastname: He
authors.forename: Lin
authors.initials: L
authors.lastname: Ma
authors.forename: Jin
authors.initials: J
authors.lastname: Ouyang
authors.forename: Fubin
authors.initials: F
authors.lastname: Jiang
mesh_terms: D001798:Blood Proteins / Q000032:analysis*; D004247:DNA / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005456:Fluorescent Dyes; D006801:Humans; D049329:Nanostructures / Q000737:chemistry*; D012834:Silver
chemical_list: D001798:Blood Proteins; D005456:Fluorescent Dyes; D012834:Silver; D004247:DNA