title: Ontology-based malaria parasite stage and species identification from peripheral blood smear images.
abstract: The diagnosis and treatment of malaria infection requires detecting the presence of the malaria parasite in the patient as well as identification of the parasite species. We present an image processing-based approach to detect parasites in microscope images of a blood smear and an ontology-based classification of the stage of the parasite for identifying the species of infection. This approach is patterned after the diagnosis approach adopted by a pathologist for visual examination, and hence, is expected to deliver similar results. We formulate several rules based on the morphology of the basic components of a parasite, namely, chromatin dot(s) and cytoplasm, to identify the parasite stage and species. Numerical results are presented for data taken from various patients. A sensitivity of 88% and a specificity of 95% is reported by evaluation of the scheme on 55 images.
authors.affiliation: Philips Research Asia - Bangalore, Philips Innovation Campus, Philips Electronics India Limited, Manyata Tech Park, Nagavara, Bangalore 560 045, Karnataka, India. vishnu.makkapati@philips.com
authors.forename: Vishnu V
authors.initials: VV
authors.lastname: Makkapati
authors.forename: Raghuveer M
authors.initials: RM
authors.lastname: Rao
mesh_terms: D000818:Animals; D048430:Cell Shape; D002843:Chromatin / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004912:Erythrocytes / Q000469:parasitology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007091:Image Processing, Computer-Assisted; D008288:Malaria / Q000097:blood / Q000175:diagnosis / Q000469:parasitology*; D008853:Microscopy / Q000379:methods*; D015233:Models, Statistical; D010963:Plasmodium falciparum / Q000378:metabolism; D010965:Plasmodium malariae / Q000378:metabolism; D041122:Plasmodium ovale / Q000378:metabolism; D010966:Plasmodium vivax / Q000378:metabolism; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D002843:Chromatin