title: A thermally baffled device for highly stabilized convective PCR.
abstract: Rayleigh-Bénard convective PCR is a simple and effective design for amplification of DNA. Convective PCR is, however, extremely sensitive to environmental temperature fluctuations, especially when using small- diameter test tubes. Therefore, this method is inherently unstable with limited applications. Here, we present a convective PCR device that has been modified by adding thermal baffles. With this thermally baffled device the influence from fluctuations in environmental temperature were significantly reduced, even in a wind tunnel (1 m/s). The thermally baffled PCR instrument described here has the potential to be used as a low-cost, point-of-care device for PCR-based molecular diagnostics in the field.
authors.affiliation: Department of Research and Development, GeneReach Biotechnology Corporation, Taichung, Taiwan.
authors.forename: Hsiao-Fen Grace
authors.initials: HF
authors.lastname: Chang
authors.forename: Yun-Long
authors.initials: YL
authors.lastname: Tsai
authors.forename: Chuan-Fu
authors.initials: CF
authors.lastname: Tsai
authors.forename: Ching-Ko
authors.initials: CK
authors.lastname: Lin
authors.forename: Pei-Yu
authors.initials: PY
authors.lastname: Lee
authors.forename: Ping-Hua
authors.initials: PH
authors.lastname: Teng
authors.forename: Chen
authors.initials: C
authors.lastname: Su
authors.forename: Chien-Chung
authors.initials: CC
authors.lastname: Jeng
mesh_terms: D018481:Convection; D018076:DNA, Complementary / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D004867:Equipment Design; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000295:instrumentation* / Q000379:methods*; D046828:Roniviridae / Q000235:genetics; D013696:Temperature; D013997:Time Factors
chemical_list: D018076:DNA, Complementary