title: Glucose/O2 biofuel cell based on enzymes, redox mediators, and multiple-walled carbon nanotubes deposited by AC-electrophoresis then stabilized by electropolymerized polypyrrole.
abstract: In this study, we developed an automated strategy to manufacture an enzyme BFC powered by glucose/O(2). The bioanode consists of GOx enzyme and PQQ redox mediator adsorbed over night on MWCNTs then deposited by means of AC-electrophoresis at 30 Hz and 160 V(p-p) and, finally stabilized by electropolymerized polypyrrole. The biocathode is constructed from LAc enzyme and ABTS redox mediator adsorbed over night on MWCNTs, then electrophoretically deposited under AC-electric field at 30 Hz and 160 V(p-p) and, finally stabilized by electrodeposited polypyrrole. The BFC was studied under air in phosphate buffer solution pH 7.4 containing 10 mM glucose and in human serum with 5 mM glucose addition at the physiological temperature of 37°C. Under these conditions, the maximum power density reaches 1.1 µW · mm(-2) at a cell voltage of 0.167 V in buffer solution and 0.69 µW · mm(-2) at cell voltage of 0.151 V in human serum. Such automated BFCs have a great potential to be optimized, miniaturized to micro and nanoscale devices suitable for in vivo studies.
authors.affiliation: Department of Metallurgy and Materials Engineering (MTM), K.U Leuven, Kasteelpark Arenberg 44, B-3001, Heverlee, Belgium. m78ammam@yahoo.fr
authors.forename: Malika
authors.initials: M
authors.lastname: Ammam
authors.forename: Jan
authors.initials: J
authors.lastname: Fransaer
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology*; D001674:Bioelectric Energy Sources; D004566:Electrodes; D004586:Electrophoresis; D005947:Glucose / Q000378:metabolism*; D005949:Glucose Oxidase / Q000378:metabolism*; D042845:Laccase / Q000378:metabolism*; D037742:Nanotubes, Carbon / Q000737:chemistry; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*; D058105:Polymerization; D011108:Polymers / Q000737:chemistry; D011758:Pyrroles / Q000737:chemistry; D055454:Trametes / Q000201:enzymology*
chemical_list: D037742:Nanotubes, Carbon; D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole; D005949:Glucose Oxidase; D042845:Laccase; D005947:Glucose; D010100:Oxygen