title: SDS can be utilized as an amyloid inducer: a case study on diverse proteins.
abstract: Sodium dodecyl sulphate (SDS), an anionic surfactant that mimics some characteristics of biological membrane has also been found to induce aggregation in proteins. The present study was carried out on 25 diverse proteins using circular dichroism, fluorescence spectroscopy, dye binding assay and electron microscopy. It was found that an appropriate molar ratio of protein to SDS readily induced amyloid formation in all proteins at a pH below two units of their respective isoelectric points (pI) while no aggregation was observed at a pH above two units of pI. We also observed that electrostatic interactions play a leading role in the induction of amyloid. This study can be used to design or hypothesize a molecule or drug, which may counter act the factor responsible for amyloid formation.
authors.affiliation: Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, India.
authors.forename: Javed Masood
authors.initials: JM
authors.lastname: Khan
authors.forename: Atiyatul
authors.initials: A
authors.lastname: Qadeer
authors.forename: Sumit Kumar
authors.initials: SK
authors.lastname: Chaturvedi
authors.forename: Ejaz
authors.initials: E
authors.lastname: Ahmad
authors.forename: Syed Arif Abdul
authors.initials: SA
authors.lastname: Rehman
authors.forename: Samudrala
authors.initials: S
authors.lastname: Gourinath
authors.forename: Rizwan Hasan
authors.initials: RH
authors.lastname: Khan
mesh_terms: D000682:Amyloid / Q000378:metabolism*; D000818:Animals; D052160:Benzothiazoles; D002942:Circular Dichroism; D005453:Fluorescence; D006801:Humans; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D007526:Isoelectric Point; D008027:Light; D008954:Models, Biological; D009391:Nephelometry and Turbidimetry; D011485:Protein Binding / Q000187:drug effects; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D012542:Scattering, Radiation; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology*; D013057:Spectrum Analysis; D013844:Thiazoles / Q000378:metabolism
chemical_list: D000682:Amyloid; D052160:Benzothiazoles; D011506:Proteins; D013844:Thiazoles; C009462:thioflavin T; D012967:Sodium Dodecyl Sulfate