title: In vitro antimycobacterial activity and HPLC-DAD screening of phenolics from Ficus benjamina L. and Ficus luschnathiana (Miq.) Miq. leaves.
abstract: The total phenolic content (Folin-Ciocalteu) of the leaves of Ficus benjamina and Ficus luschnathiana was evaluated and screened by HPLC-DAD. Ficus luschnathiana crude extract (CE) presented phenolic content higher than that of F. benjamina (149.92 ± 3.65 versus 122.63 ± 2.79 mg of GAE). Kaempferol (1.63 ± 0.16 mg g(-1) dry weight of CE) and chlorogenic acid (17.77 ± 0.57 mg g(-1) of butanolic fraction) were identified and quantified in F. benjamina, whereas rutin (1.39 ± 0.20 mg g(-1)), caffeic (1.14 ± 0.13 mg g(-1)) and chlorogenic (3.73 ± 0.29 mg g(-1)) acids were quantified in the CE of F. luschnathiana. Additionaly, rutin (15.55 ± 1.92 mg g(-1)) and quercetin (3.53 ± 0.12 mg g(-1)) were quantified in ethyl acetate and butanolic fractions, respectively. Antimycobacterial activity of CEs and fractions was evaluated against Mycobacterium smegmatis by broth microdilution method. Ethyl acetate fraction from F. benjamina and n-butanol fraction from F. luschnathiana displayed the highest inhibitory activity (MIC = 312.50 µg mL(-1) and 156.25 µg mL(-1), respectively). Further studies are required to identify the compounds directly related to antimycobacterial activity.
authors.affiliation: Department of Industrial Pharmacy, Federal University of Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Ritiel Corrêa
authors.initials: RC
authors.lastname: da Cruz
authors.forename: Vanessa
authors.initials: V
authors.lastname: Agertt
authors.forename: Aline Augusti
authors.initials: AA
authors.lastname: Boligon
authors.forename: Vanessa
authors.initials: V
authors.lastname: Janovik
authors.forename: Marli Matiko
authors.initials: MM
authors.lastname: Anraku de Campos
authors.forename: Dominique
authors.initials: D
authors.lastname: Guillaume
authors.forename: Margareth Linde
authors.initials: ML
authors.lastname: Athayde
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D002726:Chlorogenic Acid / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D030681:Ficus / Q000737:chemistry*; D044949:Kaempferols / Q000032:analysis; D008826:Microbial Sensitivity Tests; D020102:Mycobacterium smegmatis / Q000187:drug effects*; D010636:Phenols / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D011794:Quercetin / Q000032:analysis
chemical_list: D000900:Anti-Bacterial Agents; D044949:Kaempferols; D010636:Phenols; D010936:Plant Extracts; D002726:Chlorogenic Acid; D011794:Quercetin