title: The serum metabolite response to diet intervention with probiotic acidified milk in irritable bowel syndrome patients is indistinguishable from that of non-probiotic acidified milk by 1H NMR-based metabonomic analysis.
abstract: The effects of a probiotic acidified milk product on the blood serum metabolite profile of patients suffering from Irritable Bowel Syndrome (IBS) compared to a non-probiotic acidified milk product was investigated using (1)H NMR metabonomics. For eight weeks, IBS patients consumed 0.4 L per day of a probiotic fermented milk product or non-probiotic acidified milk. Both diets resulted in elevated levels of blood serum L-lactate and 3-hydroxybutyrate. Our results showed identical effects of acidified milk consumption independent of probiotic addition. A similar result was previously obtained in a questionnaire-based evaluation of symptom relief. A specific probiotic effect is thus absent both in the patient subjective symptom evaluations and at the blood serum metabolite level. However, there was no correspondence between symptom relief and metabolite response on the patient level.
authors.affiliation: Department of Food Science, Faculty of Agricultural Sciences, Aarhus University, Tjele, Denmark. simonmm.pedersen@gmail.com
authors.forename: Simon M M
authors.initials: SM
authors.lastname: Pedersen
authors.forename: Niels Chr
authors.initials: NC
authors.lastname: Nielsen
authors.forename: Henrik J
authors.initials: HJ
authors.lastname: Andersen
authors.forename: Johan
authors.initials: J
authors.lastname: Olsson
authors.forename: Magnus
authors.initials: M
authors.lastname: Simrén
authors.forename: Lena
authors.initials: L
authors.lastname: Ohman
authors.forename: Ulla
authors.initials: U
authors.lastname: Svensson
authors.forename: Anders
authors.initials: A
authors.lastname: Malmendal
authors.forename: Hanne C
authors.initials: HC
authors.lastname: Bertram
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000097:blood*; D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D015415:Biomarkers / Q000097:blood; D005260:Female; D006801:Humans; D043183:Irritable Bowel Syndrome / Q000097:blood / Q000178:diet therapy*; D019344:Lactic Acid / Q000097:blood*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D008875:Middle Aged; D008892:Milk / Q000737:chemistry*; D019936:Probiotics / Q000008:administration & dosage*; D055815:Young Adult
chemical_list: D015415:Biomarkers; D019344:Lactic Acid; D020155:3-Hydroxybutyric Acid