title: [Effect of recombinant trichosanthin on proliferation of human cevical cancer Caski cells].
abstract: OBJECTIVE
  To observe the effects of high expression of recombinant trichosanthin (rTCS) on the cell proliferation and cell cycle of human cervical cancer Caski cells.
  
  
  METHODS
  Eukaryotic expression plasmid pcDNA3.1(-)/6His-TCS was constracted and stably transfected into Caski cells. RT-PCR,Western-blot were used to select the clones with rTCS high-expressing. Using pcDNA3.1(-)-transfected cells as the control, MTT assay and flowcytometry were used to elucidate the effects of rTCS high expression on cell growth and cycle regulation in Caski cells.
  
  
  RESULTS
  The Caski cells with stable high expression of rTCS was successfully established, which could inhibit the cell growth (P<0.01) and arrest Caski cells in G1 and G2 phases (P<0.05) obviously.
  
  
  CONCLUSIONS
  High expression of rTCS can inhibit the growth of Caski cervical cancer cells, which might provide a new pathway for the therapy of cervical cancer.
authors.affiliation: Second Clinical College of China Three Gorges University, Yichang 443002, China.
authors.forename: Pingping
authors.initials: P
authors.lastname: Peng
authors.forename: Liming
authors.initials: L
authors.lastname: Huang
authors.forename: Yanlin
authors.initials: Y
authors.lastname: Wang
authors.forename: Chengcheng
authors.initials: C
authors.lastname: You
authors.forename: Weihong
authors.initials: W
authors.lastname: Cao
authors.forename: Huamei
authors.initials: H
authors.lastname: Song
authors.forename: Hanxing
authors.initials: H
authors.lastname: Tan
authors.forename: Yanzhen
authors.initials: Y
authors.lastname: Wu
mesh_terms: D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D006801:Humans; D011994:Recombinant Proteins / Q000494:pharmacology; D014162:Transfection / Q000379:methods; D015978:Trichosanthin / Q000494:pharmacology*; D002583:Uterine Cervical Neoplasms / Q000473:pathology*
chemical_list: D011994:Recombinant Proteins; D015978:Trichosanthin