title: Penaeus monodon tropomyosin induces CD4 T-cell proliferation in shrimp-allergic patients.
abstract: Shellfish allergy affects approximately 2% of the population and can cause immediate hypersensitivity reactions such as urticaria, swelling, difficulty breathing, and, in some cases, anaphylaxis. Tropomyosin is the major shrimp allergen and binds IgE in two-thirds of patients. A total of 38 shrimp-allergic patients and 20 negative control subjects were recruited and evaluated on the basis of history, skin prick testing, specific immunoglobulin E (IgE) levels, and peripheral blood mononuclear cell proliferation in response to shrimp tropomyosin or shrimp tropomyosin-derived peptides. Of the classically allergic patients by history, 59% tested positive for serum shrimp IgE antibodies. Of patients with shrimp-specific IgE in sera, 70% also had significant IgE levels specific for shrimp tropomyosin. Peripheral blood mononuclear cells from classically shrimp-allergic patients proliferated in a dose-dependent manner in response to to tropomyosin. In addition, a T-cell line derived from a shrimp-allergic patient proliferated specifically in response to tropomyosin-derived peptides. These studies suggest a strategy for immunotherapy using a tropomyosin-derived T-cell epitope vaccination.
authors.affiliation: Department of Dermatology, University of Utah, Salt Lake City, Utah, USA.
authors.forename: Shuping
authors.initials: S
authors.lastname: Wang
authors.forename: Julio C
authors.initials: JC
authors.lastname: Delgado
authors.forename: Eugene
authors.initials: E
authors.lastname: Ravkov
authors.forename: David D
authors.initials: DD
authors.lastname: Eckels
authors.forename: Ann
authors.initials: A
authors.lastname: Georgelas
authors.forename: Igor Y
authors.initials: IY
authors.lastname: Pavlov
authors.forename: Matthew
authors.initials: M
authors.lastname: Cusick
authors.forename: Kate
authors.initials: K
authors.lastname: Sebastian
authors.forename: Gerald J
authors.initials: GJ
authors.lastname: Gleich
authors.forename: Lori A
authors.initials: LA
authors.lastname: Wagner
mesh_terms: D000485:Allergens / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D005512:Food Hypersensitivity / Q000175:diagnosis / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology*; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000276:immunology*; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D012882:Skin Tests; D014335:Tropomyosin / Q000737:chemistry / Q000276:immunology*
chemical_list: D000485:Allergens; D010455:Peptides; D014335:Tropomyosin; D007073:Immunoglobulin E