title: [Viability of autologous fat grafts harvested with the Coleman technique and the tissu trans system (shippert method): a comparative study].
abstract: Various methods for harvesting and refining autologous fat grafts have been described. One of the standard procedures, the Coleman technique, is based on manual aspiration to reduce the negative presssure and the centrifugation of the grafts. The Shippert technique uses automatic liposuction with reduced negative pressure and abstains from centifugation in order not to reduce viability of the graft by exposing it to centrifugal forces. This study intends to compare the viability of fat grafts processed with the above-mentioned methods.Fat grafts were obtained in 9 patients by using both the Tissu Trans system (Shippert technique) and the Coleman technique. To evaluate the impact of centrifugation forces, the grafts harvested with the Coleman technique were treated with standard adjustment of the centrifuge and also with doubled g-force. Viability of fat grafts was analysed with the WST-8 test and with annexin V/PI assay FACS analysis.The viability of fat grafts processed by the Coleman technique was significantly higher compared to the Shippert technique on applying the WST-8 test. Applying the annexin V/PI analysis, the viability of fat grafts was almost equal with both techniques. Whereas the fat grafts processed with the Tissu Trans system are injected without condensation, the grafts refined with the Coleman technique were concentrated 3 times by centrifugation compared to the primary liposuctioned graft volumes.The Coleman technique allows the preparation of a fat graft containing more viable cells than the Shippert technique. This is in part due to the condensation of the graft by centrifugation using the Coleman technique. The factor of condensation of the grafts harvested and refined with the Coleman technique exceeds the factor of increased fat graft viability in comparison to the Shippert technique. The Tissu Trans system is more than twice as fast and easier to use with a preferential use for large volume grafts like in breast augmentation, whereas the Coleman technique produces a more condensed graft, favouring it for fat grafting to the face where less volume is needed.
authors.affiliation: Medizinische Hochschule Hannover, Klinik für Plastische, Hand- und Wiederherstellungschirurgie, Zentrum für Schwerbrandverletzte, Hannover. christianherold@gmx.de
authors.forename: C
authors.initials: C
authors.lastname: Herold
authors.forename: M
authors.initials: M
authors.lastname: Pflaum
authors.forename: P
authors.initials: P
authors.lastname: Utz
authors.forename: M
authors.initials: M
authors.lastname: Wilhelmi
authors.forename: H-O
authors.initials: HO
authors.lastname: Rennekampff
authors.forename: P M
authors.initials: PM
authors.lastname: Vogt
mesh_terms: D000273:Adipose Tissue / Q000637:transplantation*; D000293:Adolescent; D000328:Adult; D000368:Aged; D017304:Annexin A5 / Q000032:analysis; D002470:Cell Survival / Q000502:physiology; D005260:Female; D005434:Flow Cytometry; D006085:Graft Survival / Q000502:physiology*; D006801:Humans; D015187:Lipectomy / Q000295:instrumentation / Q000379:methods; D008297:Male; D008875:Middle Aged; D019651:Plastic Surgery Procedures / Q000379:methods; D020858:Tissue and Organ Harvesting / Q000295:instrumentation* / Q000379:methods*; D055815:Young Adult
chemical_list: D017304:Annexin A5