title: Association of pandemic Vibrio parahaemolyticus O3:K6 present in the coastal environment of Northwest Mexico with cases of recurrent diarrhea between 2004 and 2010.
abstract: In 2004, more than 1,230 cases of gastroenteritis due to pandemic O3:K6 strains of Vibrio parahaemolyticus were reported in southern Sinaloa, a state in Northwestern Mexico. Recurrent sporadic cases arose from 2004 to 2010, spreading from the south to the north. In the present study, Vibrio parahaemolyticus was detected in both environmental samples and clinical cases along the Pacific coast of Sinaloa during 2004 to 2010. An evaluation was made of the serotypes, distribution of virulence genes, and presence of pandemic O3:K6 strains. A total of 144 strains were isolated from environmental samples (from sediment, seawater, and shrimp), and 154 clinical strains were isolated. A total of 10 O serogroups and 30 serovars were identified in the strains. Environmental strains (n = 144) belonged to 10 O serogroups and 28 serovars, while clinical strains (n = 154) belonged to 8 O serogroups and 14 serovars. Ten serovars were shared by both environmental and clinical strains. Among 144 environmental isolates, 4.1% (6/144) belonged to the pandemic clone, with 83.3% containing the orf8 gene and with O3:K6 accounting for 67%. On the other hand, pathogenic strains (tdh and/or trh) accounted for 52% (75/144) of the environmental isolates. Interestingly, among 154 clinical isolates, 80.5% (124/154) were pandemic strains, with O3:K6 (tdh, toxRS(new), and orf8) representing the predominant serovar (99.2%, 123/124). Overall, our results indicate that in spite of a high serodiversity and prevalence of pathogenic Vibrio parahaemolyticus in the environment, the pandemic strain O3:K6 caused >79% of reported cases between 2004 and 2010 in Sinaloa, Mexico.
authors.affiliation: Sección de posgrado, Escuela Superior de Medicina IPN, Mexico City, Mexico.
authors.forename: Jorge
authors.initials: J
authors.lastname: Velazquez-Roman
authors.forename: Nidia
authors.initials: N
authors.lastname: León-Sicairos
authors.forename: Héctor
authors.initials: H
authors.lastname: Flores-Villaseñor
authors.forename: Santiago
authors.initials: S
authors.lastname: Villafaña-Rauda
authors.forename: Adrian
authors.initials: A
authors.lastname: Canizalez-Roman
mesh_terms: D016000:Cluster Analysis; D003967:Diarrhea / Q000453:epidemiology / Q000382:microbiology; D004783:Environmental Microbiology; D005759:Gastroenteritis / Q000453:epidemiology* / Q000382:microbiology*; D008800:Mexico / Q000453:epidemiology; D012008:Recurrence; D012703:Serotyping; D014735:Vibrio Infections / Q000453:epidemiology* / Q000382:microbiology*; D014736:Vibrio parahaemolyticus / Q000145:classification / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D037521:Virulence Factors / Q000235:genetics
chemical_list: D037521:Virulence Factors