title: Characterization of skeletal alterations in a model of prematurely aging mice.
abstract: An age-related bone loss occurs, apparently associated with the concomitant increase in an oxidative stress situation. However, the underlying mechanisms of age-related osteopenia are ill defined since these studies are time consuming and require the use of many animals (mainly rodents). Here, we aimed to characterize for the first time the bone status of prematurely aging mice (PAM), which exhibit an increased oxidative stress. Tibiae from adult (6 months) PAM show an increase in bone mineral density (BMD) and bone mineral content (assessed by bone densitometry) versus those in their normal counterparts (non-prematurely aging mice, NPAM) and similarly decreased in both kinds of mouse with age. However, at this bone site, trabecular BMD (determined by μ-computerized tomography) was similar in both adult PAM and old (18 months) NPAM. Femurs from these groups of mice present an increase in oxidative stress, inflammation, osteoclastogenic, and adipogenic markers, but a decrease in the gene expression of osteoblastic differentiation markers and of the Wnt/β-catenin pathway. Our findings show that adult PAM recapitulate various age-related bone features, and thus are a suitable model for premature bone senescence studies.
authors.affiliation: Bone and Mineral Metabolism Laboratory, Instituto de Investigación Sanitaria (IIS)-Fundación Jiménez Díaz, Avda. Reyes Católicos, 2, 28040, Madrid, Spain. sportal@fjd.es
authors.forename: Sergio
authors.initials: S
authors.lastname: Portal-Núñez
authors.forename: Rashed
authors.initials: R
authors.lastname: Manassra
authors.forename: Daniel
authors.initials: D
authors.lastname: Lozano
authors.forename: Alicia
authors.initials: A
authors.lastname: Acitores
authors.forename: Francisca
authors.initials: F
authors.lastname: Mulero
authors.forename: María L
authors.initials: ML
authors.lastname: Villanueva-Peñacarrillo
authors.forename: Mónica
authors.initials: M
authors.lastname: De la Fuente
authors.forename: Pedro
authors.initials: P
authors.lastname: Esbrit
mesh_terms: D015502:Absorptiometry, Photon; D019588:Aging, Premature / Q000378:metabolism*; D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western; D015519:Bone Density; D005260:Female; D005269:Femur / Q000000981:diagnostic imaging / Q000378:metabolism; D051379:Mice; D010024:Osteoporosis / Q000000981:diagnostic imaging / Q000378:metabolism*; D018384:Oxidative Stress; D060888:Real-Time Polymerase Chain Reaction; D018709:Statistics, Nonparametric; D013977:Tibia / Q000000981:diagnostic imaging / Q000378:metabolism; D055114:X-Ray Microtomography