title: Immunodetection of granzyme B tissue distribution and cellular localisation.
abstract: Investigation of Granzyme B (GrB) function and pathophysiology in both human settings and rodent models increasingly involve the use of indirect immunofluorescence imaging and fluorescence-activated cell sorting, which requires reliable GrB antibodies that do not recognise other closely related granzymes. Here, we describe the validation (using a set of recombinant granzymes, and GrB-deficient cells) and application of widely available monoclonal antibodies to specifically monitor GrB in human or mouse cells.
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, Clayton, VIC, Australia.
authors.forename: Catherina H
authors.initials: CH
authors.lastname: Bird
authors.forename: Corrine
authors.initials: C
authors.lastname: Hitchen
authors.forename: Mark
authors.initials: M
authors.lastname: Prescott
authors.forename: Ian
authors.initials: I
authors.lastname: Harper
authors.forename: Phillip I
authors.initials: PI
authors.lastname: Bird
mesh_terms: D000818:Animals; D015153:Blotting, Western; D018929:Cell Culture Techniques; D005434:Flow Cytometry / Q000379:methods; D053804:Granzymes / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods*; D021381:Protein Transport; D014018:Tissue Distribution
chemical_list: D053804:Granzymes