title: IspG-catalyzed positional isotopic exchange in methylerythritol cyclodiphosphate of the deoxyxylulose phosphate pathway: mechanistic implications.
abstract: H(2)(18)O under the bridge: Recently, the deoxyxylulose phosphate (DXP) pathway was discovered to be a second pathway supplying isoprenoid biosynthetic precursors. One of steps is an IspG-catalyzed reductive deoxygenation of methylerythritol cyclodiphosphate (MEcPP) to 4-hydroxyl-3-methyl-2-(E)-1-diphosphate (HMBPP). Using [2-(13) C,(18) O]-MEcPP, we detected the positional isotopic exchange for the bridging oxygen in MEcPP.
authors.affiliation: Department of Chemistry, Boston University, Boston, MA 02215, USA.
authors.forename: Youli
authors.initials: Y
authors.lastname: Xiao
authors.forename: Debra
authors.initials: D
authors.lastname: Rooker
authors.forename: Quincy
authors.initials: Q
authors.lastname: You
authors.forename: Caren L Freel
authors.initials: CL
authors.lastname: Meyers
authors.forename: Pinghua
authors.initials: P
authors.lastname: Liu
mesh_terms: D019747:Aldose-Ketose Isomerases / Q000737:chemistry* / Q000378:metabolism; D019883:Alkyl and Aryl Transferases / Q000737:chemistry* / Q000378:metabolism; D002384:Catalysis; D003903:Deuterium; D004896:Erythritol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D008958:Models, Molecular; D015394:Molecular Structure; D009097:Multienzyme Complexes / Q000737:chemistry* / Q000378:metabolism; D010088:Oxidoreductases / Q000737:chemistry* / Q000378:metabolism; D010103:Oxygen Isotopes; D014994:Xylose / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism
chemical_list: D009097:Multienzyme Complexes; D010103:Oxygen Isotopes; C492551:deoxyxylulose phosphate; C443879:methyl-D-erythritol 2,4-cyclodiphosphate; D014994:Xylose; D003903:Deuterium; D010088:Oxidoreductases; C114231:1-deoxy-D-xylulose 5-phosphate reductoisomerase; D019883:Alkyl and Aryl Transferases; C488494:(E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase, Nicotiana benthamiana; D019747:Aldose-Ketose Isomerases; D004896:Erythritol