title: A C-di-GMP-proflavine-hemin supramolecular complex has peroxidase activity--implication for a simple colorimetric detection.
abstract: Herein, we demonstrate that the bacterial signaling molecule, c-di-GMP, can enhance the peroxidation of hemin when proflavine is present. The c-di-GMP-proflavine-hemin nucleotidezyme can oxidize the colorless compound 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), ABTS, to the colored radical cation ABTS˙(+) and hence provides simple colorimetric detection of c-di-GMP at low micromolar concentrations.
authors.affiliation: Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA.
authors.forename: Shizuka
authors.initials: S
authors.lastname: Nakayama
authors.forename: Kevin
authors.initials: K
authors.lastname: Roelofs
authors.forename: Vincent T
authors.initials: VT
authors.lastname: Lee
authors.forename: Herman O
authors.initials: HO
authors.lastname: Sintim
mesh_terms: D052160:Benzothiazoles / Q000737:chemistry / Q000378:metabolism; D003124:Colorimetry; D006152:Cyclic GMP / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D006427:Hemin; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000737:chemistry* / Q000378:metabolism; D011370:Proflavine / Q000737:chemistry* / Q000378:metabolism; D013451:Sulfonic Acids / Q000737:chemistry / Q000378:metabolism
chemical_list: D052160:Benzothiazoles; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C062025:bis(3',5')-cyclic diguanylic acid; D006427:Hemin; D011370:Proflavine; D009195:Peroxidase; D006152:Cyclic GMP