title: Poly(galactaramidoamine) is an efficient cationic polymeric non-viral vector with low cytotoxicity for transfecting human embryonic kidney (HEK293) and murine macrophage (RAW264.7) cells.
abstract: Poly(galactaramidoamine) (PGAA) is a cationic co-polymer of dimethyl-meso-galactarate and pentaethylenehexamine. PGAA electrostatically complexes with plasmid DNA (pDNA) to form nano-sized particles. In this study, we show that PGAA-pDNA polyplexes generate high transfection efficiencies in human embryonic kidney (HEK293) and murine macrophage-like (RAW264.7) cell lines. PGAA-pDNA mediated transfection is a function of the amine:phosphate (N/P) ratio at which the polyplexes are prepared. The maximum expression of luciferase was obtained using polyplexes prepared at an N/P ratio of 40. Polyplexes prepared at increasing N/P ratios did not significantly increase in size but did result in decreasing luciferase expression. Cellular toxicity increased as the N/P ratios at which the polyplexes were prepared increased.
authors.affiliation: Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, University of Iowa , Iowa City, Iowa 5242, USA.
authors.forename: Amaraporn
authors.initials: A
authors.lastname: Wongrakpanich
authors.forename: Vijaya B
authors.initials: VB
authors.lastname: Joshi
authors.forename: Aliasger K
authors.initials: AK
authors.lastname: Salem
mesh_terms: D000818:Animals; D002412:Cations / Q000737:chemistry; D002460:Cell Line; D004247:DNA / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D010957:Plasmids / Q000737:chemistry; D011073:Polyamines / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D055672:Static Electricity; D013400:Sugar Acids / Q000737:chemistry*; D014162:Transfection / Q000379:methods
chemical_list: D002412:Cations; D004337:Drug Carriers; D011073:Polyamines; D011108:Polymers; D013400:Sugar Acids; D004247:DNA; C031122:pentaethylenehexamine; C000090:galactaric acid