title: Central adaptation to odorants depends on PI3K levels in local interneurons of the antennal lobe.
abstract: We have previously shown that driving PI3K levels up or down leads to increases or reductions in the number of synapses, respectively. Using these tools to assay their behavioral effects in Drosophila melanogaster, we showed that a loss of synapses in two sets of local interneurons, GH298 and krasavietz, leads to olfaction changes toward attraction or repulsion, while the simultaneous manipulation of both sets of neurons restored normal olfactory indexes. We show here that olfactory central adaptation also requires the equilibrated changes in both sets of local interneurons. The same genetic manipulations directed to projection (GH146) or mushroom body (201Y, MB247) neurons did not affect adaptation. Also, we show that the equilibrium is a requirement for the glomerulus-specific size changes which are a morphological signature of central adaptation. Since the two sets of local neurons are mostly, although not exclusively, inhibitory (GH298) and excitatory (krasavietz), we interpret that the normal phenomena of sensory perception, measured as an olfactory index, and central adaptation rely on an inhibition/excitation ratio.
authors.affiliation: Cajal Institute, Consejo Superior de Investigaciones Científicas, Madrid 28002, Spain. a.acebes@cajal.csic.es
authors.forename: Angel
authors.initials: A
authors.lastname: Acebes
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Devaud
authors.forename: Mercedes
authors.initials: M
authors.lastname: Arnés
authors.forename: Alberto
authors.initials: A
authors.lastname: Ferrús
mesh_terms: D000222:Adaptation, Physiological / Q000502:physiology*; D000818:Animals; D057506:Arthropod Antennae / Q000201:enzymology / Q000294:innervation; D001921:Brain / Q000166:cytology / Q000196:embryology / Q000201:enzymology*; D004331:Drosophila melanogaster / Q000502:physiology*; D019706:Excitatory Postsynaptic Potentials / Q000502:physiology; D053444:Inhibitory Postsynaptic Potentials / Q000502:physiology; D007395:Interneurons / Q000166:cytology / Q000201:enzymology*; D008297:Male; D009434:Neural Pathways / Q000166:cytology / Q000196:embryology / Q000201:enzymology; D009812:Odorants; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D012903:Smell / Q000502:physiology*; D013569:Synapses / Q000201:enzymology; D009435:Synaptic Transmission / Q000502:physiology; D014855:Wallerian Degeneration / Q000201:enzymology / Q000235:genetics
chemical_list: D019869:Phosphatidylinositol 3-Kinases