title: Anti-cellular and immunomodulatory potential of aqueous extract of Rhodiola imbricata rhizome.
abstract: In the present study, we have evaluated the anti-cellular and immunomodulatory potential of aqueous extract of Rhodiola imbricata rhizome (RAE). Rhodiola extract inhibited the proliferation of human T cell lymphoma cell line EL-4 and erythroleukemic cell line HL-60. Furthermore, treatment of human peripheral blood mononuclear cells (hPBMCs) with lipopolysaccharide (LPS) and RAE suppressed regulated upon activation, normal T cell expressed and secreted (RANTES) production. However, number of TNF-α spots was increased in RAE treated hPBMCs. The reverse transcriptase polymerase chain reaction (RT-PCR) analysis of RAE treated rat splenocytes confirmed the up regulation of TLR-4 mRNA expression. Therefore, the present study concludes that RAE has potent immune boosting activity which might be useful in immunocompromised individuals.
authors.affiliation: Immunomodulation laboratory, Defence Institute of Physiology and Allied Sciences, Timarpur, Delhi, India. kpmpgi@rediffmail.com
authors.forename: K P
authors.initials: KP
authors.lastname: Mishra
authors.forename: Lilly
authors.initials: L
authors.lastname: Ganju
authors.forename: S B
authors.initials: SB
authors.lastname: Singh
mesh_terms: D000818:Animals; D018946:Chemokine CCL5 / Q000096:biosynthesis / Q000276:immunology; D018922:HL-60 Cells; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000276:immunology; D051381:Rats; D017207:Rats, Sprague-Dawley; D027343:Rhizome / Q000737:chemistry*; D029771:Rhodiola / Q000737:chemistry*; D051197:Toll-Like Receptor 4 / Q000096:biosynthesis / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000276:immunology
chemical_list: C483024:CCL5 protein, human; D018946:Chemokine CCL5; D008070:Lipopolysaccharides; D010936:Plant Extracts; D012333:RNA, Messenger; C495345:TLR4 protein, human; C495347:Tlr4 protein, rat; D051197:Toll-Like Receptor 4; D014409:Tumor Necrosis Factor-alpha