title: Quantitative assessment of the embryotoxic potential of NSO-heterocyclic compounds using zebrafish (Danio rerio).
abstract: Heterocyclic aromatic compounds (NSO-HET) have frequently been detected in the environment. Several studies have concluded that NSO-HET pose a threat to organisms in waters, sediments and soils. However, few publications are available assessing the ecotoxicology of NSO-HET. The present study aims to assess the embryo toxicity of heterocycles using Danio rerio. A combination of the Fish Embryo Toxicity Test and analytical quantification should aid to determine the hazard potential. Changes of the total concentrations due to sorption or volatility were quantified by GC/MS. Loss of compounds during the test was observed primarily for volatile or hydrophobic NSO-HET. The LC50 calculated with nominal concentrations underestimates the toxicity by a factor up to 16 (2 h), demonstrating that a chemical analysis for comparing nominal and measured concentrations is essential for such investigations.
authors.affiliation: Department of Ecosystem Analysis, Institute for Environmental Research, RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Peddinghaus
authors.forename: Markus
authors.initials: M
authors.lastname: Brinkmann
authors.forename: Kerstin
authors.initials: K
authors.lastname: Bluhm
authors.forename: Anne
authors.initials: A
authors.lastname: Sagner
authors.forename: Gunnar
authors.initials: G
authors.lastname: Hinger
authors.forename: Thomas
authors.initials: T
authors.lastname: Braunbeck
authors.forename: Adolf
authors.initials: A
authors.lastname: Eisenträger
authors.forename: Andreas
authors.initials: A
authors.lastname: Tiehm
authors.forename: Henner
authors.initials: H
authors.lastname: Hollert
authors.forename: Steffen H
authors.initials: SH
authors.lastname: Keiter
mesh_terms: D000818:Animals; D004625:Embryo, Nonmammalian / Q000187:drug effects; D004785:Environmental Pollutants / Q000633:toxicity*; D006571:Heterocyclic Compounds / Q000633:toxicity*; D007928:Lethal Dose 50; D008954:Models, Biological; D021281:Quantitative Structure-Activity Relationship; D013723:Teratogens / Q000633:toxicity*; D015027:Zebrafish
chemical_list: D004785:Environmental Pollutants; D006571:Heterocyclic Compounds; D013723:Teratogens