title: Established preblastocyst- and blastocyst-derived ES cell lines have highly similar gene expression profiles, despite their differing requirements for derivation culture conditions.
abstract: The efficiency of embryonic stem (ES) cell derivation relies on an optimized culture medium and techniques for treating preimplantation stage embryos. Recently, ES cell derivation from the preblastocyst developmental stage was reported by removing the zona pellucida from embryos of the most efficient strain for ES cell derivation (129Sv) during early preimplantation. Here, we showed that ES cells can be efficiently derived and maintained in a modified medium (MEMα), from preblastocysts of a low-efficiency mouse strain (a hybrid consisting of 50% B6, 25% CBA, and 25% DBA). Preblastocyst-derived ES cell lines were normal in terms of pluripotency-related protein expression, and chromosome number. Also, preblastocyst-derived ES cell lines from various culture conditions showed pluripotency in vivo through teratoma analysis. Interestingly, ES cell lines produced from preblastocysts and blastocysts, regardless of the derivation culture conditions, are nearly indistinguishable by their global gene expression profiles.
authors.affiliation: Center for Regenerative Biology, University of Connecticut, Storrs, Connecticut 06269-4243, USA. chulkim@uchicago.edu
authors.forename: Chul
authors.initials: C
authors.lastname: Kim
authors.forename: Joonghoon
authors.initials: J
authors.lastname: Park
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Amano
authors.forename: Ren-He
authors.initials: RH
authors.lastname: Xu
authors.forename: Ge
authors.initials: G
authors.lastname: Lin
authors.forename: Mark G
authors.initials: MG
authors.lastname: Carter
authors.forename: Xiuchun Cindy
authors.initials: XC
authors.lastname: Tian
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D003470:Culture Media / Q000494:pharmacology; D047108:Embryonic Development / Q000187:drug effects; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D049452:Green Fluorescent Proteins / Q000378:metabolism; D059785:Karyotype; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D009028:Morula / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D050814:Octamer Transcription Factor-3 / Q000378:metabolism; D013724:Teratoma
chemical_list: D003470:Culture Media; D050814:Octamer Transcription Factor-3; C494174:Pou5f1 protein, mouse; D049452:Green Fluorescent Proteins