title: The impact of glutathione transferase kappa deficiency on adiponectin multimerisation in vivo.
abstract: Glutathione transferase Kappa (GSTK1-1) also termed disulfide bond-forming oxidoreductase A-like protein (DsbA-L) has been implicated in the post-translational multimerization of adiponectin and has been negatively correlated with obesity in mice and humans. We investigated adiponectin in Gstk1(-/-) mice and surprisingly found no difference in the levels of total serum adiponectin or the level of high molecular weight (HMW) multimers when compared with normal controls. Non-reducing SDS-polyacrylamide gel electrophoresis and western blotting also showed a similar distribution of low, middle and HMW multimers in normal and Gstk1(-/-) mice. Variation in adiponectin has been correlated with glucose tolerance and with the levels of phosphorylated AMP-kinase but we found similar glucose tolerance and similar levels of phospho 5-AMP-activated protein kinase in normal and Gstk1(-/-) mice. Consequently, our findings suggest that GSTK1-1 is not absolutely required for adiponectin multimerization in vivo and alternate pathways may be activated in GSTK1-1 deficiency.
authors.affiliation: The John Curtin School of Medical Research, Australian National University, Canberra, ACT, Australia.
authors.forename: A
authors.initials: A
authors.lastname: Theodoratos
authors.forename: A C
authors.initials: AC
authors.lastname: Blackburn
authors.forename: M
authors.initials: M
authors.lastname: Coggan
authors.forename: J
authors.initials: J
authors.lastname: Cappello
authors.forename: C Z
authors.initials: CZ
authors.lastname: Larter
authors.forename: K I
authors.initials: KI
authors.lastname: Matthaei
authors.forename: P G
authors.initials: PG
authors.lastname: Board
mesh_terms: D041721:3T3-L1 Cells / Q000378:metabolism; D052242:Adiponectin / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D059305:Diet, High-Fat; D005982:Glutathione Transferase / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D008970:Molecular Weight; D009765:Obesity / Q000235:genetics / Q000378:metabolism*
chemical_list: D052242:Adiponectin; D005982:Glutathione Transferase; C568627:glutathione transferase kappa, mouse