title: Multilocus genotyping of Giardia duodenalis in lambs from Spain reveals a high heterogeneity.
abstract: Fecal specimens from 120 lambs in Valencia (Spain) were analyzed for Giardia duodenalis by IFA and nested-PCR using the beta giardin (bg), glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi) and small subunit ribosomal RNA (ssurRNA) genes. The highest prevalence was obtained using the ssurRNA gene (89.2%), whereas values from other techniques ranged from 64.1% to 69.2%. Sequences of the ssurRNA showed a high proportion of assemblage A or mixed assemblage A/E samples (55.1% and 25.2%, respectively). When the other 3 loci were analyzed, between 6.5% and 15.4% were found to be assemblage A or A/E, respectively. Nested PCR for the tpi gene was the most variable of the targets employed. Twelve new sequences of gdh and tpi for G. duodenalis from sheep were found. Multilocus genotyping resulted in 63 patterns from the 71 samples sequenced at the four loci. This high variability among isolates possibly reflects the high frequency of mixed infections.
authors.affiliation: Departamento de Producción Animal, Sanidad Animal y Ciencia y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad CEU Cardenal-Herrera, Avenida Seminario S/N, 46113 Moncada, Valencia, Spain. mariateresa.gomez.munoz@pdi.ucm.es
authors.forename: María Teresa
authors.initials: MT
authors.lastname: Gómez-Muñoz
authors.forename: Carmen
authors.initials: C
authors.lastname: Cámara-Badenes
authors.forename: María del Carmen
authors.initials: Mdel C
authors.lastname: Martínez-Herrero
authors.forename: María Auxiliadora
authors.initials: MA
authors.lastname: Dea-Ayuela
authors.forename: María Teresa
authors.initials: MT
authors.lastname: Pérez-Gracia
authors.forename: Salceda
authors.initials: S
authors.lastname: Fernández-Barredo
authors.forename: Mónica
authors.initials: M
authors.lastname: Santín
authors.forename: Ronald
authors.initials: R
authors.lastname: Fayer
mesh_terms: D000818:Animals; D005243:Feces / Q000469:parasitology; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation / Q000502:physiology; D014644:Genetic Variation; D005838:Genotype; D016829:Giardia lamblia / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism; D012756:Sheep; D012757:Sheep Diseases / Q000453:epidemiology / Q000469:parasitology*; D013030:Spain / Q000453:epidemiology
chemical_list: D015800:Protozoan Proteins