title: Stylissamide X, a new proline-rich cyclic octapeptide as an inhibitor of cell migration, from an Indonesian marine sponge of Stylissa sp.
abstract: A new proline-rich cyclic octapeptide named stylissamide X (1) was isolated from an Indonesian marine sponge of Stylissa sp. as an inhibitor of cell migration from the guidance of wound-healing assay. The chemical structure of stylissamide X (1) was determined on the basis of spectroscopic analysis, and stereostructure of the amino acids were deduced by Marfey's method. Compound 1 showed inhibitory activity against migration of HeLa cells in the ranges of 0.1-10 μM concentration through both wound-healing assay and chemotaxicell chamber assay, while the cell viability was maintained more than 75% up to 10 μM concentration of 1.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, Japan. araim@phs.osaka-u.ac.jp
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Arai
authors.forename: Yoshi
authors.initials: Y
authors.lastname: Yamano
authors.forename: Mayumi
authors.initials: M
authors.lastname: Fujita
authors.forename: Andi
authors.initials: A
authors.lastname: Setiawan
authors.forename: Motomasa
authors.initials: M
authors.lastname: Kobayashi
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010456:Peptides, Cyclic / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D011161:Porifera / Q000737:chemistry*; D011392:Proline / Q000737:chemistry*
chemical_list: D010456:Peptides, Cyclic; C572321:stylissamide X; D011392:Proline