title: Study on the chaperone properties of conserved GTPases.
abstract: As a large family of hydrolases, GTPases are widespread in cells and play the very important biological function of hydrolyzing GTP into GDP and inorganic phosphate through binding with it. GTPases are involved in cell cycle regulation, protein synthesis, and protein transportation. Chaperones can facilitate the folding or refolding of nascent peptides and denatured proteins to their native states. However, chaperones do not occur in the native structures in which they can perform their normal biological functions. In the current study, the chaperone activity of the conserved GTPases of Escherichia coli is tested by the chemical denaturation and chaperone-assisted renaturation of citrate synthase and α-glucosidase. The effects of ribosomes and nucleotides on the chaperone activity are also examined. Our data indicate that these conserved GTPases have chaperone properties, and may be ancestral protein folding factors that have appeared before dedicated chaperones.
authors.affiliation: RNA Laboratory, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Wang
authors.forename: Jiaying
authors.initials: J
authors.lastname: Xue
authors.forename: Zhe
authors.initials: Z
authors.lastname: Sun
authors.forename: Yan
authors.initials: Y
authors.lastname: Qin
authors.forename: Weimin
authors.initials: W
authors.lastname: Gong
mesh_terms: D002950:Citrate (si)-Synthase / Q000737:chemistry; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004926:Escherichia coli / Q000166:cytology / Q000201:enzymology*; D020558:GTP Phosphohydrolases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D006153:Guanosine Diphosphate / Q000494:pharmacology; D006160:Guanosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D018832:Molecular Chaperones / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D011489:Protein Denaturation / Q000187:drug effects; D020673:Protein Renaturation / Q000187:drug effects; D012270:Ribosomes / Q000378:metabolism; D000520:alpha-Glucosidases / Q000737:chemistry
chemical_list: C503923:GDPNP compound; D018832:Molecular Chaperones; D006153:Guanosine Diphosphate; D006160:Guanosine Triphosphate; D002950:Citrate (si)-Synthase; D000520:alpha-Glucosidases; D020558:GTP Phosphohydrolases