title: Comparative study using phenotypic, genotypic, and proteomics methods for identification of coagulase-negative staphylococci.
abstract: Five methods were compared to determine the most accurate method for identification of coagulase-negative staphylococci (CoNS) (n = 142 strains). Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) showed the best results for rapid and accurate CoNS differentiation (99.3% of strains correctly identified). An alternative to this approach could be Vitek2 combined with partial tuf gene sequencing (100% of strains correctly identified when both methods are performed simultaneously).
authors.affiliation: PAMM Laboratories, Department of Medical Microbiology, Eindhoven-Veldhoven, The Netherlands.
authors.forename: Anne J M
authors.initials: AJ
authors.lastname: Loonen
authors.forename: Arjan R
authors.initials: AR
authors.lastname: Jansz
authors.forename: Jeandery N B
authors.initials: JN
authors.lastname: Bergland
authors.forename: Marion
authors.initials: M
authors.lastname: Valkenburg
authors.forename: Petra F G
authors.initials: PF
authors.lastname: Wolffs
authors.forename: Adriaan J C
authors.initials: AJ
authors.lastname: van den Brule
mesh_terms: D003030:Coagulase / Q000235:genetics*; D005838:Genotype; D058889:Molecular Typing; D010641:Phenotype; D040901:Proteomics; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012015:Reference Standards; D017422:Sequence Analysis, DNA / Q000592:standards; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000592:standards; D013210:Staphylococcus / Q000145:classification* / Q000201:enzymology / Q000235:genetics
chemical_list: D003030:Coagulase; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S