title: Comparasion of five gene loci (rnpB, 16S rRNA, 16S-23S rRNA, sodA and dnaJ) to aid the molecular identification of viridans-group streptococci and pneumococci.
abstract: Viridans-group streptococci (VGS) consist of several taxa which historically have been highly diverse. However, at times it may become necessary to have a reliable scheme for the identification of these organisms to the species level. The aim of this study is to compare the ability of five gene loci, namely rnpB, 16S rRNA, 16S-23S rRNA, sodA and dnaJ, to speciate such organisms through a sequence typing-based approach. Reference organisms consisting of six VGS species were compared based on sequence typing, followed by comparison of 31 wild-type respiratory isolates, and showed that employment of sequence typing using the rnpB gene locus was the most specific and reliable. Therefore, the use of rnpB sequencing for the identification of VGS to species level is a reliable and feasible option, based on a single gene target.
authors.affiliation: Northern Ireland Public Health Laboratory, Department of Bacteriology, Belfast City Hospital, Belfast, Northern Ireland, UK.
authors.forename: Y
authors.initials: Y
authors.lastname: Maeda
authors.forename: C E
authors.initials: CE
authors.lastname: Goldsmith
authors.forename: W A
authors.initials: WA
authors.lastname: Coulter
authors.forename: C
authors.initials: C
authors.lastname: Mason
authors.forename: J S G
authors.initials: JS
authors.lastname: Dooley
authors.forename: C J
authors.initials: CJ
authors.lastname: Lowery
authors.forename: B C
authors.initials: BC
authors.lastname: Millar
authors.forename: J E
authors.initials: JE
authors.lastname: Moore
mesh_terms: D005798:Genes, Bacterial / Q000235:genetics*; D006801:Humans; D010802:Phylogeny; D011008:Pneumococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D013280:Stomatitis / Q000175:diagnosis* / Q000382:microbiology; D013290:Streptococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D013296:Streptococcus pneumoniae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D034363:Viridans Streptococci / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S