title: Phenolic composition and antioxidant effect of aqueous extract of Arisaema cum Bile, the Oriental Herb Medicine, in human fibroblast cells.
abstract: Phenolic composition and antioxidant activities of the aqueous extract of Arisaema cum Bile, which is widely used as a folk medicine in Korea, were determined. Phenolic composition profile revealed that the aqueous extract is rich in sinapic acid (13.14 mg/100 g extract), catechin (9.88 mg/100 g extract), neohesperidin (7.38 mg/100 g extract), and chlorogenic acid (3.64 mg/100 g extract). The aqueous extract effectively scavenged toward 2,2-diphenyl-1-picrylhydrazyl (90.63%), hydrogen peroxide (98.13%), and hydroxyl radical (59.62%) at 2.0 mg/mL, and also showed high reducing power. In cytotoxic evaluation, the aqueous extract exhibited no significant cytotoxicity in human fibroblast, and it also exhibited appreciable suppression of intracellular reactive oxygen species and inhibition of lipid peroxidation. In addition, the aqueous extract upregulated the level of glutathione in a dose-dependent manner. Taken together, the aqueous extract of Arisaema cum Bile could be considered as a potential natural source that may be useful for curing diseases arising from oxidative deterioration.
authors.affiliation: School of Food Technology and Nutrition, Chonnam National University, Yeosu, Republic of Korea.
authors.forename: Chang-Bum
authors.initials: CB
authors.lastname: Ahn
authors.forename: Tai-Sun
authors.initials: TS
authors.lastname: Shin
authors.forename: Hye Kyoung
authors.initials: HK
authors.lastname: Seo
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Je
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D031057:Arisaema / Q000737:chemistry*; D001713:Biphenyl Compounds / Q000494:pharmacology; D002460:Cell Line; D045424:Complex Mixtures / Q000737:chemistry / Q000494:pharmacology; D003373:Coumaric Acids / Q000737:chemistry / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D017665:Hydroxyl Radical / Q000494:pharmacology; D010084:Oxidation-Reduction / Q000187:drug effects; D010851:Picrates / Q000494:pharmacology
chemical_list: D000890:Anti-Infective Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D045424:Complex Mixtures; D003373:Coumaric Acids; D010851:Picrates; D017665:Hydroxyl Radical; C073734:sinapinic acid; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl