title: Biological activities of ACL-I and physicochemical properties of ACL-II, lectins isolated from the marine sponge Axinella corrugata.
abstract: Lectin II from the marine sponge Axinella corrugata (ACL-II) was purified by affinity chromatography on rabbit erythrocytic stroma incorporated into a polyacrylamide gel, followed by gel filtration on Ultrogel AcA 44 column. Purified ACL-II is a lectin with an Mr of 80 kDa and 78 kDa, estimated by SDS-PAGE and by FPLC on Superose 12 HR column, respectively. ACL-II mainly agglutinates native rabbit erythrocytes and this hemagglutinating activity is independent of Ca(2+), Mg(2+) and Mn(2+), but is inhibited by d-galactose, chitin and N-acetyl derivatives, with the exception of GalNAc. ACL-II is stable for up to 65 °C for 30 min, with a better stability at a pH range of 2 to 6. In contrast, ACL-I displays a strong mitogenic and cytotoxic effect.
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, 90610-000, Porto Alegre, RS, Brazil. rrdresch@gmail.com
authors.forename: Roger R
authors.initials: RR
authors.lastname: Dresch
authors.forename: Cléa B
authors.initials: CB
authors.lastname: Lerner
authors.forename: Beatriz
authors.initials: B
authors.lastname: Mothes
authors.forename: Vera M T
authors.initials: VM
authors.lastname: Trindade
authors.forename: Amélia T
authors.initials: AT
authors.lastname: Henriques
authors.forename: Magdolna M
authors.initials: MM
authors.lastname: Vozári-Hampe
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D047089:Axinella / Q000737:chemistry*; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000187:drug effects; D006386:Hemagglutination Tests; D006801:Humans; D006863:Hydrogen-Ion Concentration; D037102:Lectins / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D011817:Rabbits; D012394:Rosaniline Dyes; D013997:Time Factors
chemical_list: D037102:Lectins; D012394:Rosaniline Dyes; C004692:coomassie Brilliant Blue