title: Abnormal flow adhesion of sickle red blood cells to human placental trophoblast extracellular matrix.
abstract: Pregnancy in sickle cell disease (SCD) has been associated with increased complications such as vaso-occlusive crises, severe anemia and foetal loss. It has been proposed that the sickling of red blood cells (RBCs) inside the placenta circulation could participate to these complications. The present study investigated the adhesion of sickle RBCs on human trophoblast-derived cell and its extracellular matrix. Results demonstrated 1) similar adhesion of sickle RBCs and healthy RBCs to trophoblast but 2) a greater adhesion of sickle RBCs to the extracellular matrix of trophoblasts as compared with healthy RBCs. This greater adhesion could partly involve the Lu/BCAM glycoproteins and could participate to the complications reported in SCD pregnant women.
authors.affiliation: Inserm 665, Université des Antilles et de la Guyane, Pointe-à-Pitre, Guadeloupe.
authors.forename: Yann
authors.initials: Y
authors.lastname: Lamarre
authors.forename: Stéphane
authors.initials: S
authors.lastname: Petres
authors.forename: Marie-Dominique
authors.initials: MD
authors.lastname: Hardy-Dessources
authors.forename: Stéphane
authors.initials: S
authors.lastname: Sinnapah
authors.forename: Marc
authors.initials: M
authors.lastname: Romana
authors.forename: Sandrine
authors.initials: S
authors.lastname: Laurance
authors.forename: Nathalie
authors.initials: N
authors.lastname: Lemonne
authors.forename: Jürg
authors.initials: J
authors.lastname: Gysin
authors.forename: Philippe
authors.initials: P
authors.lastname: Connes
mesh_terms: D000328:Adult; D000755:Anemia, Sickle Cell / Q000150:complications*; D002448:Cell Adhesion; D002460:Cell Line; D004907:Erythrocyte Deformability; D004912:Erythrocytes / Q000473:pathology*; D005109:Extracellular Matrix / Q000378:metabolism; D005260:Female; D006801:Humans; D010920:Placenta / Q000098:blood supply*; D011247:Pregnancy; D011250:Pregnancy Complications, Hematologic / Q000097:blood* / Q000378:metabolism / Q000473:pathology; D014327:Trophoblasts / Q000166:cytology*