title: The patatin-containing phospholipase A pPLAIIα modulates oxylipin formation and water loss in Arabidopsis thaliana.
abstract: The patatin-related phospholipase A (pPLA) hydrolyzes membrane glycerolipids to produce monoacyl compounds and free fatty acids. Phospholipids are cleaved by pPLAIIα at the sn-1 and sn-2 positions, and galactolipids, including those containing oxophytodienoic acids, can also serve as substrates. Ablation of pPLAIIα decreased lysophosphatidylcholine and lysophosphatidylethanolamine levels, but increased free linolenic acid. pPLAIIα-deficient plants displayed a higher level of jasmonic acid and methyl jasmonate, as well as the oxylipin-biosynthetic intermediates 13-hydroperoxylinolenic acid and 12-oxophytodienoic acid than wild-type (WT) plants. The expression of genes involved in oxylipin production was also higher in the pPLAIIα-deficient mutant than in WT plants. The mutant plants lost water more quickly than WT plants. The stomata of WT and mutant plants responded similarly to abscisic acid. In response to desiccation, the mutant and WT leaves produced abscisic acid at the same rate, but, after 4 h of desiccation, the jasmonic acid level was much higher in mutant than WT leaves. These results indicate that pPLAIIα negatively regulates oxylipin production and suggest a role in the removal of oxidatively modified fatty acids from membranes.
authors.affiliation: Department of Biology, University of Missouri, St Louis, MO 63121, USA.
authors.forename: Wen-Yu
authors.initials: WY
authors.lastname: Yang
authors.forename: Yong
authors.initials: Y
authors.lastname: Zheng
authors.forename: Sung Chul
authors.initials: SC
authors.lastname: Bahn
authors.forename: Xiang-Qing
authors.initials: XQ
authors.lastname: Pan
authors.forename: Mao-Yin
authors.initials: MY
authors.lastname: Li
authors.forename: Hieu Sy
authors.initials: HS
authors.lastname: Vu
authors.forename: Mary R
authors.initials: MR
authors.lastname: Roth
authors.forename: Brad
authors.initials: B
authors.lastname: Scheu
authors.forename: Ruth
authors.initials: R
authors.lastname: Welti
authors.forename: Yue-Yun
authors.initials: YY
authors.lastname: Hong
authors.forename: Xue-Min
authors.initials: XM
authors.lastname: Wang
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D017360:Arabidopsis / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D003517:Cyclopentanes / Q000378:metabolism; D055864:Droughts; D038983:Galactolipids / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D055786:Gene Knockout Techniques; D006867:Hydrolases; D006868:Hydrolysis / Q000187:drug effects; D008246:Lysophospholipids / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D054883:Oxylipins / Q000378:metabolism*; D010641:Phenotype; D010741:Phospholipases A / Q000235:genetics / Q000378:metabolism*; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D054046:Plant Stomata / Q000187:drug effects / Q000502:physiology; D013379:Substrate Specificity / Q000187:drug effects; D015854:Up-Regulation / Q000187:drug effects; D014867:Water / Q000378:metabolism*; D000217:Acyltransferases; D010740:Phospholipases
chemical_list: D000040:Abscisic Acid; D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D038983:Galactolipids; D006867:Hydrolases; D008246:Lysophospholipids; D054883:Oxylipins; D010741:Phospholipases A; D014867:Water; C011006:jasmonic acid; C507690:At2g26560 protein, Arabidopsis; D000217:Acyltransferases; D010740:Phospholipases