title: Cell surface biotinylation by azaelectrocyclization: easy-handling and versatile approach for living cell labeling.
abstract: Versatile method for living cell labeling has been established. Cell surfaces are initially biotinylated by azaelectrocyclization, and then treated with the fluorescence-labeled avidin or the anti-biotin antibody.
authors.affiliation: Department of Chemistry, Graduate School of Science, Osaka University, 1-1 Machikaneyama-cho, Toyonaka-shi, Osaka 560-0043, Japan.
authors.forename: Katsunori
authors.initials: K
authors.lastname: Tanaka
authors.forename: Satomi
authors.initials: S
authors.lastname: Yokoi
authors.forename: Koji
authors.initials: K
authors.lastname: Morimoto
authors.forename: Takayuki
authors.initials: T
authors.lastname: Iwata
authors.forename: Yuka
authors.initials: Y
authors.lastname: Nakamoto
authors.forename: Kaori
authors.initials: K
authors.lastname: Nakayama
authors.forename: Koichi
authors.initials: K
authors.lastname: Koyama
authors.forename: Takeshi
authors.initials: T
authors.lastname: Fujiwara
authors.forename: Koichi
authors.initials: K
authors.lastname: Fukase
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D001360:Avidin / Q000737:chemistry*; D001710:Biotin / Q000737:chemistry* / Q000276:immunology; D019921:Biotinylation; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry* / Q000648:ultrastructure; D002470:Cell Survival; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D018613:Microscopy, Confocal / Q000379:methods; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D000906:Antibodies; D005456:Fluorescent Dyes; D001360:Avidin; D001710:Biotin