title: MiR-421 is a functional marker of circulating tumor cells in gastric cancer patients.
abstract: The detection of circulating tumor cells (CTCs) has recently received great attention. To evaluate if miR-421 could be used as a specific marker for CTCs, the level of miR-421 in mononuclear cells (MNCs) from peripheral blood were determined by reverse transcription-polymerase chain reaction. Transfection of miR-421 inhibitor significantly suppressed tumor growth in vivo. The level of miR-421 in MNCs from gastric cancer was significantly higher than in those from healthy controls. The area under the receiver operating characteristic curve was 0.773 ± 0.0736. In conclusion, miR-421 may be used as a biomarker for monitoring CTCs in patients with gastric cancer.
authors.affiliation: Institute of Biochemistry and Molecular Biology, Ningbo University School of Medicine, Ningbo, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhou
authors.forename: Bingxiu
authors.initials: B
authors.lastname: Xiao
authors.forename: Fei
authors.initials: F
authors.lastname: Zhou
authors.forename: Hongxia
authors.initials: H
authors.lastname: Deng
authors.forename: Xinjun
authors.initials: X
authors.lastname: Zhang
authors.forename: Yanru
authors.initials: Y
authors.lastname: Lou
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Gong
authors.forename: Caigan
authors.initials: C
authors.lastname: Du
authors.forename: Junming
authors.initials: J
authors.lastname: Guo
mesh_terms: D000368:Aged; D000818:Animals; D014408:Biomarkers, Tumor / Q000097:blood; D002277:Carcinoma / Q000097:blood / Q000175:diagnosis* / Q000473:pathology; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D002681:China; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000097:blood; D008875:Middle Aged; D009360:Neoplastic Cells, Circulating / Q000378:metabolism* / Q000473:pathology; D012334:RNA, Neoplasm / Q000037:antagonists & inhibitors / Q000235:genetics; D012372:ROC Curve; D012265:Ribonucleotides / Q000008:administration & dosage / Q000627:therapeutic use; D013274:Stomach Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000473:pathology; D023041:Xenograft Model Antitumor Assays
chemical_list: D014408:Biomarkers, Tumor; C547665:MIRN421 microRNA, human; D035683:MicroRNAs; D012334:RNA, Neoplasm; D012265:Ribonucleotides