title: Mediatophore regulates acetylcholine release from T cells.
abstract: Immunological stimulation of T cells by phytohemagglutinin (PHA) enhances the synthesis and release of acetylcholine (ACh), suggesting a role for the lymphocytic cholinergic system in the regulation of immune function. In the present study, we used two human leukemic T cell lines as models to investigate whether mediatophore, a homooligomer of a 16-kDa subunit homologous to the proteolipid subunit c of vacuolar H(+)-ATPase (V-ATPase), is involved in mediating ACh release from T cells. Immunohistochemical analysis revealed the presence of mediatophore in the cytoplasm and on the plasma membrane of both T cell lines. Mediatophore gene expression was up-regulated by immunological T cell activation by PHA. Transfection of anti-mediatophore small interference RNA down-regulated mediatophore gene expression and significantly reduced ACh release. These results suggest that T cells express mediatophore, which then plays a key role in mediating ACh release, and that mediatophore expression is regulated by immunological stimulation.
authors.affiliation: Department of Pharmacology, Doshisha Women's College of Liberal Arts, 97-1 Minamihokodate, Kodo, Kyotanabe, Kyoto 610-0395, Japan. tfujii@dwc.doshisha.ac.jp
authors.forename: Takeshi
authors.initials: T
authors.lastname: Fujii
authors.forename: Yuki
authors.initials: Y
authors.lastname: Takada-Takatori
authors.forename: Kazuhide
authors.initials: K
authors.lastname: Horiguchi
authors.forename: Koichiro
authors.initials: K
authors.lastname: Kawashima
mesh_terms: D000109:Acetylcholine / Q000378:metabolism*; D002460:Cell Line; D005786:Gene Expression Regulation; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects; D008934:Mitogens / Q000494:pharmacology; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010835:Phytohemagglutinins / Q000494:pharmacology; D034741:RNA, Small Interfering / Q000494:pharmacology; D013601:T-Lymphocytes / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D008934:Mitogens; D009419:Nerve Tissue Proteins; D010835:Phytohemagglutinins; D034741:RNA, Small Interfering; C050299:mediatophore; D000109:Acetylcholine