title: Signal enhancement of a micro-arrayed polydiacetylene (PDA) biosensor using gold nanoparticles.
abstract: Polydiacetylene (PDA) liposomes possess unique properties that allow liposomes to change color and emit fluorescence in response to stimuli such as temperature, antibody-antigen interaction, pH, mechanical stress, and organic solvent. They have been studied extensively as signal transducers in biosensor applications. Here, we describe an antibody-based biosensor using PDA liposomes for detection of human immunoglobulin E (hIgE). Target hIgE chemically bound to hIgE monoclonal antibodies immobilized on PDA liposomes and the fluorescent signals were slightly increased depending on the target protein concentration. As the primary response, the hIgE could be detected to below 10 ng mL(-1). However, fluorescent signals were dramatically increased depending on the target protein concentration when gold nanoparticle-conjugated polyclonal antibody probes were added on the PDA liposomes after the primary immune reaction. A PDA liposome biosensor could detect the hIgE as low as 0.1 ng mL(-1) and the sensitivity was increased up to one hundred times higher than the primary response. As a result, we confirmed that gold nanoparticle-conjugated polyclonal antibody probes efficiently enhanced the fluorescent signal of the PDA liposome biosensor chip. This strategy can be useful to detect proteins of ultra-low concentration.
authors.affiliation: School of Chemical Engineering, Sungkyunkwan University, Suwon, 440-746, Korea.
authors.forename: Sang Ho
authors.initials: SH
authors.lastname: Won
authors.forename: Sang Jun
authors.initials: SJ
authors.lastname: Sim
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis; D008081:Liposomes / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D046228:Microarray Analysis / Q000379:methods*; D010316:Particle Size; D000078789:Polyacetylene Polymer; D011108:Polymers / Q000737:chemistry*; D053279:Polyynes / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D008081:Liposomes; D000078789:Polyacetylene Polymer; D011108:Polymers; D053279:Polyynes; C082361:polydiacetylene; D007073:Immunoglobulin E; D006046:Gold