title: Helicobacter pylori serologic status has no influence on the association between fucosyltransferase 2 polymorphism (FUT2 461 G->A) and vitamin B-12 in Europe and West Africa.
abstract: BACKGROUND
  Genomewide association studies have shown a relation between plasma vitamin B-12 concentration and the 461G→A polymorphism of fucosyltransferase 2 (FUT2), a gene associated with susceptibility to Helicobacter pylori infection.
  
  
  OBJECTIVE
  We evaluated in 2 populations the association of FUT2 461 G→A polymorphism with vitamin B-12 and related metabolic markers and investigated whether the influence of FUT2 on H. pylori serology is part of the mechanisms that underlie these associations.
  
  
  METHODS
  The study included 1282 ambulatory subjects from Europe and West Africa. Blood concentrations of vitamin B-12, folate, homocysteine, and methylmalonic acid were measured. Genotyping was performed by real-time polymerase chain reaction. H. pylori serology testing was performed by using ELISA.
  
  
  RESULTS
  In univariate analysis, FUT2 461 A/A genotype was associated with higher plasma vitamin B-12 concentration in the total population (P = 0.0007) as well as in Europe (P = 0.0009) and in West Africa (P = 0.0015). Positivity for H. pylori serology was higher in West Africa (P < 0.0001) and was not associated with low plasma vitamin B-12. The prevalence of H. pylori-positive patients did not differ among FUT2 461 G→A genotypes (P = 0.2068). In multivariate analysis, FUT2 461 G→A genotype (P = 0.0008), but not positive H. pylori serology, was an independent predictor of plasma vitamin B-12 concentration.
  
  
  CONCLUSIONS
  This study confirms the influence of FUT2 461 G→A polymorphism on plasma vitamin B-12 concentration and showed no influence of H. pylori serologic status on this association in ambulatory subjects from Europe and West Africa.
authors.affiliation: INSERM U, Cellular and Molecular Pathology in Nutrition, Henri Poincaré University Nancy, Vandoeuvre-lès-Nancy, France.
authors.forename: Abderrahim
authors.initials: A
authors.lastname: Oussalah
authors.forename: Cyril
authors.initials: C
authors.lastname: Besseau
authors.forename: Céline
authors.initials: C
authors.lastname: Chery
authors.forename: Elise
authors.initials: E
authors.lastname: Jeannesson
authors.forename: Rosa-Maria
authors.initials: RM
authors.lastname: Guéant-Rodriguez
authors.forename: Guido
authors.initials: G
authors.lastname: Anello
authors.forename: Paolo
authors.initials: P
authors.lastname: Bosco
authors.forename: Maurizzio
authors.initials: M
authors.lastname: Elia
authors.forename: Antonino
authors.initials: A
authors.lastname: Romano
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Bronowicki
authors.forename: Philippe
authors.initials: P
authors.lastname: Gerard
authors.forename: Justine
authors.initials: J
authors.lastname: Paoli
authors.forename: Patrice Hodonou
authors.initials: PH
authors.lastname: Avogbe
authors.forename: Nicodème
authors.initials: N
authors.lastname: Chabi
authors.forename: Ambaliou
authors.initials: A
authors.lastname: Sanni
authors.forename: Emile
authors.initials: E
authors.lastname: Amouzou
authors.forename: Laurent
authors.initials: L
authors.lastname: Peyrin-Biroulet
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Guéant
mesh_terms: D000349:Africa; D000704:Analysis of Variance; D004797:Enzyme-Linked Immunosorbent Assay; D005060:Europe; D005260:Female; D005647:Fucosyltransferases / Q000235:genetics*; D005838:Genotype; D016481:Helicobacter Infections / Q000097:blood / Q000235:genetics*; D016480:Helicobacter pylori; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D015995:Prevalence; D014805:Vitamin B 12 / Q000097:blood*; D000097631:Galactoside 2-alpha-L-fucosyltransferase
chemical_list: D005647:Fucosyltransferases; D014805:Vitamin B 12; D000097631:Galactoside 2-alpha-L-fucosyltransferase