title: Statistical optimization of microalgae Pavlova lutheri cultivation conditions and its fermentation conditions by yeast, Candida rugopelliculosa.
abstract: In this study, sequential strategy based design was applied to optimize the microalgae, Pavlova lutheri mass culture conditions and fermentation conditions of the cultured algae by proteolytic yeast Candidia rugopelliculosa to obtain small peptide chains. This optimization of culture and fermentation conditions by response surface methodology (RSM) finally leads to effective purification of a bioactive peptide MPGPLSPL (793.01 Da) with hydroxyl radical scavenging activity. Collectively, these results indicated that microalgae P. lutheri can enhance the hydroxyl radical inhibiting effect through protein hydrolysis process under RSM optimal condition.
authors.affiliation: Marine Bioprocess Research Center, Pukyong National University, Busan 608-737, Republic of Korea.
authors.forename: BoMi
authors.initials: B
authors.lastname: Ryu
authors.forename: Kyong-Hwa
authors.initials: KH
authors.lastname: Kang
authors.forename: Dai-Hung
authors.initials: DH
authors.lastname: Ngo
authors.forename: Zhong-Ji
authors.initials: ZJ
authors.lastname: Qian
authors.forename: Se-Kwon
authors.initials: SK
authors.lastname: Kim
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D002175:Candida / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004578:Electron Spin Resonance Spectroscopy; D005285:Fermentation; D058086:Microalgae / Q000254:growth & development / Q000378:metabolism*; D059748:Proteolysis; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species