title: On-chip incubation system for long-term microfluidic cell culture.
abstract: We demonstrate the use of a microfluidic cell culture chip with Braille pin-driven pumping, capable of on-chip CO2 incubation that does not require an external chamber or gas supply. The proposed chip consists of a poly(dimethylsiloxane)(PDMS)-made microfluidic chip, flip-mounted on a glass slide, that contains a nested pair of cell culture media reservoirs and water-jacket, insulated by a permeable PDMS wall. By using 0.8 M sodium bicarbonate with 65 mM sodium carbonate as the water-jacket and placing on a 37 °C surface, the chip maintained osmolality shift and the pCO2 in the media reservoir stabilized within < 3 mmol/kg and 5.0% ± 0.2% over at least 24 hours. The incubation capabilities were demonstrated through microfluidic culture of CV-1 epithelial cells under an inverted microscope for at least 12 days.
authors.affiliation: School of Science and Engineering, Tokyo Denki University, Saitama 350-0394, Japan. a-takano3562@frontier.dendai.ac.jp
authors.forename: Atsushi
authors.initials: A
authors.lastname: Takano
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Ogawa
authors.forename: Masato
authors.initials: M
authors.lastname: Tanaka
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Futai
mesh_terms: D000818:Animals; D002245:Carbon Dioxide / Q000737:chemistry; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D009994:Osmolar Concentration; D010313:Partial Pressure; D012996:Solutions; D013997:Time Factors; D059008:Time-Lapse Imaging
chemical_list: D012996:Solutions; D002245:Carbon Dioxide