title: DNA extraction of ancient animal hard tissue samples via adsorption to silica particles.
abstract: A large number of subfossil and more recent skeletal remains, many of which are stored in museums and private collections, are potentially accessible for DNA sequence analysis. In order to extract the small amount of DNA preserved in these specimens, an efficient DNA release and purification method is required. In this chapter, I describe an efficient and straightforward purification and concentration method that uses DNA adsorption to a solid surface of silica particles. Comparative analysis of extraction methods has shown that this method works reliably for ancient as well as younger, museum-preserved specimens.
authors.affiliation: Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA. nrohland@genetics.med.harvard.edu
authors.forename: Nadin
authors.initials: N
authors.lastname: Rohland
mesh_terms: D000327:Adsorption; D000818:Animals; D001842:Bone and Bones / Q000737:chemistry; D004247:DNA / Q000302:isolation & purification*; D009144:Museums; D012822:Silicon Dioxide / Q000737:chemistry*; D014070:Tooth / Q000737:chemistry
chemical_list: D012822:Silicon Dioxide; D004247:DNA