title: No proinflammatory signature in CD34+ hematopoietic progenitor cells in multiple sclerosis patients.
abstract: Autologous hematopoietic stem cell transplantation (aHSCT) has been used as a therapeutic approach in multiple sclerosis (MS). However, it is still unclear if the immune system that emerges from autologous CD34+ hematopoietic progenitor cells (HPC) of MS patients is pre-conditioned to re-develop the proinflammatory phenotype. The objective of this article is to compare the whole genome gene and microRNA expression signature in CD34+ HPC of MS patients and healthy donors (HD). CD34+ HPC were isolated from peripheral blood of eight MS patients and five HD and analyzed by whole genome gene expression and microRNA expression microarray. Among the differentially expressed genes (DEGs) only TNNT1 reached statistical significance (logFC=3.1, p<0.01). The microRNA expression was not significantly different between MS patients and HD. We did not find significant alterations of gene expression or microRNA profiles in CD34+ HPCs of MS patients. Our results support the use of aHSCT for treatment of MS.
authors.affiliation: Institute of Neuroimmunology and Clinical MS Research, Center for Molecular Neurobiology Hamburg, Germany. andreas.lutterotti@i-med.ac.at
authors.forename: A
authors.initials: A
authors.lastname: Lutterotti
authors.forename: I
authors.initials: I
authors.lastname: Jelcić
authors.forename: C
authors.initials: C
authors.lastname: Schulze
authors.forename: S
authors.initials: S
authors.lastname: Schippling
authors.forename: P
authors.initials: P
authors.lastname: Breiden
authors.forename: B
authors.initials: B
authors.lastname: Mazzanti
authors.forename: S
authors.initials: S
authors.lastname: Reinhardt
authors.forename: M
authors.initials: M
authors.lastname: DiGioia
authors.forename: A
authors.initials: A
authors.lastname: Repice
authors.forename: L
authors.initials: L
authors.lastname: Massacesi
authors.forename: A
authors.initials: A
authors.lastname: Sputtek
authors.forename: G
authors.initials: G
authors.lastname: Salinas-Riester
authors.forename: N
authors.initials: N
authors.lastname: Kroeger
authors.forename: M
authors.initials: M
authors.lastname: Sospedra
authors.forename: R
authors.initials: R
authors.lastname: Saccardi
authors.forename: A
authors.initials: A
authors.lastname: Zander
authors.forename: R
authors.initials: R
authors.lastname: Martin
mesh_terms: D018952:Antigens, CD34 / Q000032:analysis*; D016022:Case-Control Studies; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D005838:Genotype; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D006801:Humans; D007249:Inflammation / Q000235:genetics / Q000276:immunology*; D008297:Male; D035683:MicroRNAs / Q000032:analysis*; D020528:Multiple Sclerosis, Chronic Progressive / Q000235:genetics / Q000276:immunology*; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000235:genetics / Q000276:immunology*; D010641:Phenotype; D025341:Principal Component Analysis
chemical_list: D018952:Antigens, CD34; D035683:MicroRNAs