title: Genome-wide high-resolution aCGH analysis of gestational choriocarcinomas.
abstract: Eleven samples of DNA from choriocarcinomas were studied by high resolution CGH-array 244 K. They were studied after histopathological confirmation of the diagnosis, of the androgenic etiology and after a microsatellite marker analysis confirming the absence of contamination of tumor DNA from maternal DNA. Three cell lines, BeWo, JAR, JEG were also studied by this high resolution pangenomic technique. According to aCGH analysis, the de novo choriocarcinomas exhibited simple chromosomal rearrangements or normal profiles. The cell lines showed various and complex chromosomal aberrations. 23 Minimal Critical Regions were defined that allowed us to list the genes that were potentially implicated. Among them, unusually high numbers of microRNA clusters and imprinted genes were observed.
authors.affiliation: INSERM U985, Institut Gustave Roussy, Villejuif, France.
authors.forename: Henriette
authors.initials: H
authors.lastname: Poaty
authors.forename: Philippe
authors.initials: P
authors.lastname: Coullin
authors.forename: Jean Félix
authors.initials: JF
authors.lastname: Peko
authors.forename: Philippe
authors.initials: P
authors.lastname: Dessen
authors.forename: Ange Lucien
authors.initials: AL
authors.lastname: Diatta
authors.forename: Alexander
authors.initials: A
authors.lastname: Valent
authors.forename: Eric
authors.initials: E
authors.lastname: Leguern
authors.forename: Sophie
authors.initials: S
authors.lastname: Prévot
authors.forename: Charles
authors.initials: C
authors.lastname: Gombé-Mbalawa
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Candelier
authors.forename: Jean-Yves
authors.initials: JY
authors.lastname: Picard
authors.forename: Alain
authors.initials: A
authors.lastname: Bernheim
mesh_terms: D045744:Cell Line, Tumor; D002822:Choriocarcinoma / Q000235:genetics* / Q000473:pathology; D055028:Comparative Genomic Hybridization / Q000379:methods*; D005260:Female; D015894:Genome, Human / Q000235:genetics*; D005838:Genotype; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D018895:Microsatellite Repeats / Q000235:genetics; D011247:Pregnancy; D011248:Pregnancy Complications / Q000235:genetics* / Q000473:pathology; D017422:Sequence Analysis, DNA; D014594:Uterine Neoplasms / Q000235:genetics* / Q000473:pathology