title: Genome-wide identification and characterization of a panel of house-keeping genes in Schistosoma japonicum.
abstract: Quantitative real-time polymerase chain reaction (qPCR), as one of the most sensitive and precise gene expression analysis methods, is frequently used to validate data obtained in high-through-put assays. qPCR requires reference genes with stable transcription for accurate normalization. However, no systematic studies on such genes have been performed in the genus Schistosoma japonicum. In this study, eight novel candidate genes selected from a microarray analysis and four commonly used reference genes were systematically validated in a series of qPCR experiments. Based on the results of geNorm, Normfinder, BestKeeper, and the comparative delta-cycle threshold (ΔCT) integrated analysis, the genes PSMD4, NDUFV2, and TPC2L were found to be most stably expressed in all S. japonicum developmental stages; meanwhile, ACTB and TUBA were found as the least stably expressed genes. This study provided, at the first time, data for genes that can be explored as reliable references in transcriptomic analysis of S. japonicum.
authors.affiliation: Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Liu
authors.forename: Pengfei
authors.initials: P
authors.lastname: Cai
authors.forename: Nan
authors.initials: N
authors.lastname: Hou
authors.forename: Xianyu
authors.initials: X
authors.lastname: Piao
authors.forename: Heng
authors.initials: H
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Hung
authors.forename: Qijun
authors.initials: Q
authors.lastname: Chen
mesh_terms: D000465:Algorithms; D000818:Animals; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D017238:Genes, Helminth; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D018510:RNA, Helminth / Q000235:genetics*; D011817:Rabbits; D012015:Reference Standards; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods / Q000592:standards; D012549:Schistosoma japonicum / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D018510:RNA, Helminth