title: Antiproliferative effect of alpinetin in BxPC-3 pancreatic cancer cells.
abstract: Alpinetin is a novel plant flavonoid derived from Alpinia katsumadai Hayata,             found to possess strong anticancer effects. However, the antitumor effect of alpinetin             on pancreatic cancer cells and the detailed mechanism remain unclear. The aim             of this study was to investigate alpinetin's beneficial effect on pancreatic cancer             and the possible molecular mechanism involved. Pancreatic cancer cell lines were             treated with alpinetin at various doses and for different times, and the effect             of alpinetin on cell growth inhibition, apoptosis and the cell cycle was determined.             The expression of Bcl-2, Bcl-xL, XIAP and Bax, the activity of caspases and the             levels of cytochrome c released were measured. The results showed that alpinetin             inhibited the viability of three pancreatic cancer cell lines and induced apoptosis             of BxPC-3 cells in a dose- and time-dependent manner. This was accompanied by             regulation of the expression of Bcl-2, Bcl-xL, Bax and XIAP. Furthermore, alpinetin             treatment led to the release of cytochrome c and activation of caspases-3, -8             and -9 proteins. Taken together, our studies indicate that alpinetin inhibited             the proliferation of pancreatic cancer cells possibly through the regulation of             the Bcl-2 family and XIAP expression, release of cytochrome c and the activation             of caspases. Alpinetin may serve as a potential agent for the development of pancreatic             cancer cell therapies.
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Dalian Medical University, Dalian 116011, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Du
authors.forename: Bo
authors.initials: B
authors.lastname: Tang
authors.forename: Jingwen
authors.initials: J
authors.lastname: Wang
authors.forename: Hongtao
authors.initials: H
authors.lastname: Sui
authors.forename: Xueli
authors.initials: X
authors.lastname: Jin
authors.forename: Liming
authors.initials: L
authors.lastname: Wang
authors.forename: Zhongyu
authors.initials: Z
authors.lastname: Wang
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D053181:Caspase 8 / Q000235:genetics / Q000378:metabolism; D053453:Caspase 9 / Q000235:genetics / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D045304:Cytochromes c / Q000235:genetics / Q000378:metabolism; D044950:Flavanones / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D010190:Pancreatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D051636:X-Linked Inhibitor of Apoptosis Protein / Q000235:genetics / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism; D051020:bcl-X Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D044950:Flavanones; D051636:X-Linked Inhibitor of Apoptosis Protein; C496866:XIAP protein, human; C436748:alpinetin; D051028:bcl-2-Associated X Protein; D051020:bcl-X Protein; D045304:Cytochromes c; C505407:CASP3 protein, human; C505530:CASP8 protein, human; C506433:CASP9 protein, human; D053148:Caspase 3; D053181:Caspase 8; D053453:Caspase 9