title: Phytochemical study and anti-inflammatory, antidiabetic and free radical scavenger evaluations of Krameria pauciflora methanol extract.
abstract: The plant Krameria pauciflora MOC et. Sessé ex DC. is used as an anti-inflammatory and antidiabetic in traditional medicine. The aim of this study was to evaluate the in vivo anti-inflammatory and antidiabetic effects of a methanol extract from the roots of K. pauciflora. Dichloromethane and ethyl acetate extracts obtained by partitioning the methanol extract were also evaluated. Complete methanol and dichloromethane extracts showed anti-inflammatory effects at 3 mg/kg. An anti-inflammatory effect similar to indomethacin (10 mg/kg) was observed when the methanol and dichloromethane extracts, which contain a cycloartane-type triterpene and an sterol, were administered orally at several doses (3, 10, 30 and 100 mg/kg), whereas no anti-inflammatory effect was observed at any dose for the ethyl acetate extract, which contains catechin-type flavonoids. The antidiabetic effect of each extract was also determined. An antihyperglycaemic effect was observed in diabetic rats, but no effect in normoglycaemic animals was observed when the methanol extract was administrated at 30 mg/kg. All of the extracts exhibited radical scavenger activity. Additionally, constituents from all of the extracts were identified by NMR. This article supports the use of K. pauciflora as an anti-inflammatory because it exhibits a similar effect to indomethacin. However, its antidiabetic effect is not completely clear, although it could be useful for preventing diabetic complications.
authors.affiliation: Centro de Investigaciones Químicas, Universidad Autónoma del Estado de Morelos, Avenida Universidad No. 1001 Col. Chamilpa, 62209 Cuernavaca, Morelos, Mexico. angelesrc@uaem.mx
authors.forename: M Ángeles
authors.initials: MÁ
authors.lastname: Ramírez-Cisneros
authors.forename: María Yolanda
authors.initials: MY
authors.lastname: Rios
authors.forename: Myrna
authors.initials: M
authors.lastname: Déciga-Campos
authors.forename: A Berenice
authors.initials: AB
authors.lastname: Aguilar-Guadarrama
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D019540:Area Under Curve; D052160:Benzothiazoles / Q000737:chemistry; D001713:Biphenyl Compounds / Q000737:chemistry; D001786:Blood Glucose; D002351:Carrageenan; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D005528:Foot / Q000473:pathology; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D005609:Free Radicals / Q000737:chemistry; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D031326:Krameriaceae / Q000737:chemistry*; D008297:Male; D000432:Methanol / Q000737:chemistry*; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D013451:Sulfonic Acids / Q000737:chemistry
chemical_list: D000893:Anti-Inflammatory Agents; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D001786:Blood Glucose; D016166:Free Radical Scavengers; D005609:Free Radicals; D007004:Hypoglycemic Agents; D010851:Picrates; D010936:Plant Extracts; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D002351:Carrageenan; C004931:1,1-diphenyl-2-picrylhydrazyl; D000432:Methanol