title: [Experimental study on the induced differentiation of human amnion mesenchymal cells into osteoblasts].
abstract: OBJECTIVE
  To investigate the feasibility of inducing differentiation of the human amniotic mesenchymal cells (hAMCs) into osteoblasts in vitro, so as to provide the seed cells for bone tissue engineering.
  
  
  METHODS
  The hAMCs were isolated from abandoned human amnion and cultured in osteogenic media to induce the osteogenic differentiation in vitro. After hAMCs were induced by osteogenic media for 15 days, morphological observation, immunocytochemistry and western blot were used to study the cellular morphology and expression of alkaline phosphatase (ALP), type I collagen, osteopontin and osteocalcin.
  
  
  RESULTS
  The primary cultured hAMCs had long spindle shape or irregular shape, which were distributed evenly. The cells were usually suheultured in 5 or 7 days. After subculture, the cells became larger. After cultured by osteogenic media for 15 days, the hAMCs were detected to express ALP, osteocalcin and osteopontin, and secrete type I collagen.
  
  
  CONCLUSIONS
  The hAMCs are isolated, cultured and amplified easily in vitro. The induced differentiated cells by osteogenic media have typical osteoblast morphological and functional characteristics, which can be used as seed cells for bone tissue engineering.
authors.affiliation: Institute of Clinical Medical Science, China-Japan Friendship Hospital, Beijng 100029, China 100029, China. shujun2000cn@yahoo.com.cn
authors.forename: Jun
authors.initials: J
authors.lastname: Shu
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Guo
authors.forename: Ke-Hua
authors.initials: KH
authors.lastname: Zhang
authors.forename: Zhe
authors.initials: Z
authors.lastname: Cai
authors.forename: Li-Ming
authors.initials: LM
authors.lastname: Cheng
authors.forename: Rong-Qi
authors.initials: RQ
authors.lastname: Li
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.forename: Lin
authors.initials: L
authors.lastname: Pan
authors.forename: Hua
authors.initials: H
authors.lastname: Ou
authors.forename: Xiao-Jie
authors.initials: XJ
authors.lastname: Huang
authors.forename: Xiang-Li
authors.initials: XL
authors.lastname: Zhang
authors.forename: Yang
authors.initials: Y
authors.lastname: Xu
authors.forename: Yan
authors.initials: Y
authors.lastname: Gao
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Cui
authors.forename: Hong-Xia
authors.initials: HX
authors.lastname: Zhang
authors.forename: Jia-Xin
authors.initials: JX
authors.lastname: Liu
mesh_terms: D000650:Amnion / Q000166:cytology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010006:Osteoblasts / Q000166:cytology*; D010012:Osteogenesis; D023822:Tissue Engineering / Q000379:methods