title: A general RNA motif for cellular transfection.
abstract: We have developed a selection scheme to generate nucleic acid sequences that recognize and directly internalize into mammalian cells without the aid of conventional delivery methods. To demonstrate the generality of the technology, two independent selections with different starting pools were performed against distinct target cells. Each selection yielded a single highly functional sequence, both of which folded into a common core structure. This internalization signal can be adapted for use as a general purpose reagent for transfection into a wide variety of cell types including primary cells.
authors.affiliation: Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
authors.forename: Maria L B
authors.initials: ML
authors.lastname: Magalhães
authors.forename: Michelle
authors.initials: M
authors.lastname: Byrom
authors.forename: Amy
authors.initials: A
authors.lastname: Yan
authors.forename: Linsley
authors.initials: L
authors.lastname: Kelly
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.forename: Raquel
authors.initials: R
authors.lastname: Furtado
authors.forename: Deborah
authors.initials: D
authors.lastname: Palliser
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Ellington
authors.forename: Matthew
authors.initials: M
authors.lastname: Levy
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004705:Endocytosis; D005260:Female; D005434:Flow Cytometry; D015723:Gene Library; D018014:Gene Transfer Techniques; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009092:Mucous Membrane / Q000378:metabolism; D009690:Nucleic Acid Conformation; D059372:Nucleotide Motifs; D012313:RNA / Q000737:chemistry*; D014162:Transfection; D014621:Vagina / Q000378:metabolism
chemical_list: D012313:RNA