title: The effect of rosmarinic acid on 1,2-dimethylhydrazine induced colon carcinogenesis.
abstract: This study was carried out to investigate the chemopreventive potential of rosmarinic acid (RA) against 1,2-dimethylhydrazine (DMH) induced rat colon carcinogenesis by evaluating the effect of RA on tumour formation, antioxidant enzymes, cytochrome P450 content, p-nitrophenol hydroxylase and GST activities. Rats were divided into six groups and fed modified pellet diet for the entire experimental period. Group 1 served as control, group 2 received RA (10 mg/kgb.w.). Groups 3-6 were induced colon cancer by injecting DMH (20 mg/kgb.w.) subcutaneously once a week for the first four weeks (groups 3-6). In addition, RA was administered at the doses of 2.5, 5 and 10 mg/kgb.w. to groups 4-6 respectively. DMH treated rats showed large number of colonic tumours; decreased lipid peroxidation; decreased antioxidant status; elevated CYP450 content and PNPH activities; and decreased GST activity in the liver and colon. Supplementation with RA (5 mgkg/b.w.) to DMH treated rats significantly decreased the number of polyps (50%); reversed the markers of oxidative stress (21.0%); antioxidant status (38.55%); CYP450 content (29.41%); and PNPH activities (21.9%). RA at the dose of 5 mg/kgb.w. showed a most pronounced effect and could be used as a possible chemopreventive agent against colon cancer.
authors.affiliation: Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar 608002, Tamilnadu, India.
authors.forename: Karthikkumar
authors.initials: K
authors.lastname: Venkatachalam
authors.forename: Sivagami
authors.initials: S
authors.lastname: Gunasekaran
authors.forename: Victor Antony Santiago
authors.initials: VA
authors.lastname: Jesudoss
authors.forename: Nalini
authors.initials: N
authors.lastname: Namasivayam
mesh_terms: D019813:1,2-Dimethylhydrazine / Q000633:toxicity; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology; D002273:Carcinogens / Q000633:toxicity; D002934:Cinnamates / Q000494:pharmacology*; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000517:prevention & control*; D053630:Depsides / Q000494:pharmacology*; D019587:Dietary Supplements; D004195:Disease Models, Animal; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D000097123:Rosmarinic Acid
chemical_list: D019813:1,2-Dimethylhydrazine; D000970:Antineoplastic Agents; D000975:Antioxidants; D002273:Carcinogens; D002934:Cinnamates; D053630:Depsides; D000097123:Rosmarinic Acid