title: Effect of antisense oligodeoxynucleotide targeted against NF-κB/P65 on cell proliferation and tumorigenesis of gastric cancer.
abstract: To study the inhibitory effect of nuclear transcription factor-kappa B(NF-κB) antisense oligodeoxynucleotide(ASODN) on the growth and tumorgenesis of human gastric cancer. We synthesized and transfected the ASODN of NF-κB/P65 to gastric cancer cell line. The effect of ASODN of NF-κB/P65 on the proliferation of gastric cancer cells was measured by MTT method. The subcutaneous xenograft model of human gastric cancer was established in nude mice, and the tumor growth curve was observed. The cell proliferation was significantly inhibited in P65 ASODN-transfected group in vitro (P<0.05). In vivo, tumor formation test showed that the tumor volume in nude mice in ASODN group was obviously smaller than in other groups (P<0.05); the apoptosis index (AI) was significantly higher (P<0.001). Simultaneously, MVD in ASODN group was markedly lower than in other groups (P<0.01). NF-κB could be used as a new biological therapeutic target of gastric cancer.
authors.affiliation: Department of Oncology, Shanghai East Hospital, Tongji University, Shanghai, 200120, China. Leeqi2001@hotmail.com
authors.forename: Qi
authors.initials: Q
authors.lastname: Li
authors.forename: Yong
authors.initials: Y
authors.lastname: Gao
authors.forename: Zeng-guang
authors.initials: ZG
authors.lastname: Xu
authors.forename: Hong
authors.initials: H
authors.lastname: Jiang
authors.forename: Ying-Yan
authors.initials: YY
authors.lastname: Yu
authors.forename: Zheng-Gang
authors.initials: ZG
authors.lastname: Zhu
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D004195:Disease Models, Animal; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D020319:Oligodeoxyribonucleotides, Antisense / Q000235:genetics / Q000378:metabolism*; D013194:Staining and Labeling / Q000379:methods; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000473:pathology / Q000517:prevention & control*; D013778:Tetrazolium Salts / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism; D051996:Transcription Factor RelA / Q000037:antagonists & inhibitors* / Q000235:genetics
chemical_list: D000970:Antineoplastic Agents; D020319:Oligodeoxyribonucleotides, Antisense; D013778:Tetrazolium Salts; D013844:Thiazoles; D051996:Transcription Factor RelA; C022616:thiazolyl blue