title: [Study on preservation of Fritillaria anhuiensis by vitrification in vitro].
abstract: OBJECTIVE
  To establish cryopreservation system of shoot-tips from Fritillaria anhuiensis.
  
  
  METHODS
  Taking vitrification as system of cryopreservation, the shoot tips with length 2-3 mm were precultured in MS medium enriched with 0.4 mol x L(-1) sucrose for 3 d. They were treated for 20 min with 60% PVS2 at 25 degrees C, and then subjected to ice-cooled vitrification solution for 60 min and transferred to 2 mL cryotubes with fresh vitrification solution (PVS2) and plunged into liquid nitrogen. After rapid thawing in 40 degrees C water bath for 1 min, shoot-tips were expelled into MS medium containing 1.2 mol x L(-1) sucrose for 20 min. Further recovery and growth took place on regeneration medium in the dark at 25 degrees C for 2 weeks, and then with light/dark cycle of 12/12 h. The genetic integrity of cryopreserved shoot-tips was identified through products of PCR with arbitrary primers.
  
  
  CONCLUSIONS
  The highest survival rates of shoot-tips reached 79.9% by vitrification, and the regeneration rates were 52.3%. No changes were found between treated materials and untreated materials in genomic DNA.
authors.affiliation: School of Life Science, Anhui Key Laboratory of Plant Resources and Biology, Huaibei Normal University, Huaibei 235000, China.
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Zhu
authors.forename: Fei
authors.initials: F
authors.lastname: Chen
authors.forename: Jianping
authors.initials: J
authors.lastname: Xue
authors.forename: Aimin
authors.initials: A
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Sheng
authors.forename: Yunxian
authors.initials: Y
authors.lastname: Song
mesh_terms: D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000737:chemistry; D031410:Fritillaria / Q000235:genetics / Q000378:metabolism*; D042822:Genomic Instability / Q000235:genetics; D018520:Plant Shoots / Q000235:genetics / Q000378:metabolism*; D010946:Plants, Medicinal / Q000235:genetics / Q000378:metabolism*; D011309:Preservation, Biological; D016019:Survival Analysis; D058989:Vitrification
chemical_list: D003451:Cryoprotective Agents