title: Downregulation of ferroportin 1 expression in hFOB1.19 osteoblasts by hepcidin.
abstract: The present study was designed to address the relationship between iron homeostasis and bone metabolism. Cultured hFOB 1.19 osteoblasts were incubated with selected concentrations of hepcidin (50, 100, and 200 nmol/L) for 20 h, harvested for extraction of total RNA and proteins, and the expression of ferroportin 1 was analyzed by RT-PCR and western blotting. The results showed the presence of ferroportin 1 expression in cultured hFOB 1.19 cells. Furthermore, the ferroportin 1 had a similar expression pattern in hFOB cells as in hepatocytes and enterocytes and was downregulated by hepcidin. Our data indicate that osteoblasts are target cells for hepcidin, suggest that hepcidin may have many more targets than previously recognized, and support the role of hepcidin in the development of osteoporosis.
authors.affiliation: Department of Orthopaedics, The Second Affiliated Hospital of SooChow University, Suzhou 215004, China. xuyoujia@medmail.com.cn
authors.forename: Youjia
authors.initials: Y
authors.lastname: Xu
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Xiao
authors.forename: Aidong
authors.initials: A
authors.lastname: Wang
authors.forename: Pierre
authors.initials: P
authors.lastname: Sirois
authors.forename: Kai
authors.initials: K
authors.lastname: Li
mesh_terms: D023181:Antimicrobial Cationic Peptides / Q000378:metabolism* / Q000494:pharmacology*; D027682:Cation Transport Proteins / Q000096:biosynthesis*; D002460:Cell Line; D015536:Down-Regulation; D020895:Enterocytes / Q000378:metabolism; D022781:Hepatocytes / Q000378:metabolism; D064451:Hepcidins; D006801:Humans; D010006:Osteoblasts / Q000378:metabolism*; D010024:Osteoporosis / Q000473:pathology; D000098742:Ferroportin
chemical_list: D023181:Antimicrobial Cationic Peptides; D027682:Cation Transport Proteins; C578702:HAMP protein, human; D064451:Hepcidins; D000098742:Ferroportin