title: Performance assessment of a novel two-step multiple displacement amplification-PCR assay for detection of Mycobacterium tuberculosis complex in sputum specimens.
abstract: A novel two-step multiple displacement amplification-PCR (MDA-PCR) assay for tuberculosis detection in 200 sputum specimens was evaluated. The MDA-PCR assay indicated a significant increase in sensitivity and specificity compared with those of standard PCR alone.
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Na
authors.initials: N
authors.lastname: Wu
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Fu
authors.forename: Ruifen
authors.initials: R
authors.lastname: Zhang
authors.forename: Lan
authors.initials: L
authors.lastname: Feng
authors.forename: Yongfei
authors.initials: Y
authors.lastname: Hu
authors.forename: Xiaoliang
authors.initials: X
authors.lastname: Li
authors.forename: Na
authors.initials: N
authors.lastname: Lu
authors.forename: Xiuqin
authors.initials: X
authors.lastname: Zhao
authors.forename: Yuanlong
authors.initials: Y
authors.lastname: Pan
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Baoli
authors.initials: B
authors.lastname: Zhu
authors.forename: Kanglin
authors.initials: K
authors.lastname: Wan
mesh_terms: D016022:Case-Control Studies; D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics*; D006801:Humans; D025202:Molecular Diagnostic Techniques; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013183:Sputum / Q000382:microbiology; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis* / Q000382:microbiology
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial