title: [Cloning and distribution pattern of HMGR gene conserved fragment in Alisma orientale].
abstract: OBJECTIVE
  To clone and study the distribution pattern of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) gene conserved fragment in Alisma orientale.
  
  
  METHODS
  The HMGR conserved fragment in A. orientale was amplified by RT-PCR strategy with the total RNA of young leaves as the template. HMGR mRNA expression in different organs was detected by real-time quantitative PCR (QRT-PCR).
  
  
  RESULTS
  The conserved fragment was 458 bp (accession NO. HQ913638). NCBI Blast sequence analysis showed the resulting protein had high homology to HMGR with 86.8% similarity to Artemisia annua, 88.2% to Bupleurum chinense, 88.2% to Eucommia ulmoides, and 85.5% to Salvia miltiorrhiza. The result of QRT-PCR showed that the HMGR gene was expressed in different organs (i. e. leaves, petioles, tubers, and roots) which was higher in leaves relative to other tissues.
  
  
  CONCLUSIONS
  The HMGR gene conserved fragment from A. orientale was cloned and its distribution pattern was detected for the first time. This work provides a foundation for exploring the synthetic pathway and bioengineering of Alisma terpenes.
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, China. guwei9926@126.com
authors.forename: Wei
authors.initials: W
authors.lastname: Gu
authors.forename: Bei-Li
authors.initials: BL
authors.lastname: Xi
authors.forename: Qi-Nan
authors.initials: QN
authors.lastname: Wu
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Chao
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.forename: Xiu-Yuan
authors.initials: XY
authors.lastname: Shen
mesh_terms: D034902:Alisma / Q000201:enzymology* / Q000235:genetics*; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D019295:Computational Biology; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics*; D006903:Hydroxymethylglutaryl CoA Reductases / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics; D018517:Plant Roots / Q000201:enzymology / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014315:Triterpenes / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D014315:Triterpenes; D006903:Hydroxymethylglutaryl CoA Reductases