title: Green-to-red photoconvertible mEosFP-aided live imaging in plants.
abstract: Numerous subcellular-targeted probes have been created using a monomeric green-to-red photoconvertible Eos fluorescent protein for understanding the growth and development of plants. These probes can be used to create color-based differentiation between similar cells, differentially label organelle subpopulations, and track subcellular structures and their interactions. Both green and red fluorescent forms of mEosFP are stable and compatible with single colored FPs. Differential highlighting using mEosFP probes greatly increases spatiotemporal precision during live imaging.
authors.affiliation: Department of Molecular and Cellular Biology, Laboratory of Plant Development and Interactions, University of Guelph, Guelph, Canada.
authors.forename: Jaideep
authors.initials: J
authors.lastname: Mathur
authors.forename: Sarah
authors.initials: S
authors.lastname: Griffiths
authors.forename: Kiah
authors.initials: K
authors.lastname: Barton
authors.forename: Martin H
authors.initials: MH
authors.lastname: Schattat
mesh_terms: D017360:Arabidopsis / Q000166:cytology; D058948:Cell Tracking / Q000295:instrumentation / Q000379:methods*; D036681:Fluorescence Recovery After Photobleaching / Q000379:methods; D049452:Green Fluorescent Proteins / Q000032:analysis* / Q000737:chemistry; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008164:Luminescent Proteins / Q000032:analysis* / Q000737:chemistry; D059828:Plant Cells; D030821:Plants, Genetically Modified / Q000166:cytology; D000097573:Red Fluorescent Protein
chemical_list: D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein