title: Prevalence of 16S rRNA methylase genes in Enterobacteriaceae isolates from a Greek university hospital.
abstract: 16S ribosomal RNA methylase-mediated high-level resistance to 4-,6-aminoglycosides has been reported in clinical isolates of gram-negative bacilli from several countries. Three of 1534 (0.2%) isolates of Klebsiella pneumoniae and three of 734 (0.4%) Proteus mirabilis isolates from a university hospital in Athens, Greece, were positive for rmtB and highly resistant to all aminoglycosides tested (MICs ≥256 mg/L). Two of the K. pneumoniae rmtB-bearing isolates, were KPC-2 and OXA-10 producers and the third was a DHA-1 producer. One of the P. mirabilis isolates was a VIM-1 and OXA-10 producer and one was an OXA-10 producer. All rmtB-harbouring isolates were clonally unrelated. None of the E. coli (n = 1398) and Enterobacter spp. (n = 414) isolates were positive for armA, rmtA, rmtB, rmtC or rmtD.
authors.affiliation: 4th Department of Internal Medicine, Infectious Disease Laboratory, Molecular Biology Section, Athens University School of Medicine, Chaidari, Greece. egalani@med.uoa.gr
authors.forename: I
authors.initials: I
authors.lastname: Galani
authors.forename: M
authors.initials: M
authors.lastname: Souli
authors.forename: T
authors.initials: T
authors.lastname: Panagea
authors.forename: G
authors.initials: G
authors.lastname: Poulakou
authors.forename: K
authors.initials: K
authors.lastname: Kanellakopoulou
authors.forename: H
authors.initials: H
authors.lastname: Giamarellou
mesh_terms: D000617:Aminoglycosides / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D004755:Enterobacteriaceae / Q000201:enzymology* / Q000302:isolation & purification*; D004756:Enterobacteriaceae Infections / Q000453:epidemiology* / Q000382:microbiology*; D005798:Genes, Bacterial; D006115:Greece / Q000453:epidemiology; D006785:Hospitals, University; D006801:Humans; D008780:Methyltransferases / Q000235:genetics* / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D015995:Prevalence; D012336:RNA, Ribosomal, 16S / Q000378:metabolism
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D012336:RNA, Ribosomal, 16S; D008780:Methyltransferases; C035181:rRNA (adenosine-O-2'-)methyltransferase