title: Effects of graded levels of dietary methionine hydroxy analogue on immune response and antioxidant status of immune organs in juvenile Jian carp (Cyprinus carpio var. Jian).
abstract: Immune response and antioxidant status of immune organs in juvenile Jian carp (Cyprinus carpio var. Jian) fed graded levels of methionine hydroxy analogue (MHA) (0, 5.1, 7.6, 10.2, 12.7, 15.3 g kg(-1) diet) for 60 days were investigated. Results indicated that head kidney index, spleen index, red and white blood cell counts significantly increased with increasing MHA levels up to a point (P < 0.05), whereupon decreased (P < 0.05). Glutathione reductase activity in head kidney and spleen, anti-hydroxy radical and glutathione-S-transferase activities in spleen, catalase activity and GSH content in head kidney significantly increased by MHA supplement, while malondialdehyde content, anti-superoxide anion, superoxide dismutase, glutathione peroxidase activities in head kidney and spleen, protein carbonyl content and catalase activity in spleen, anti-hydroxy radical activity in head kidney significantly decreased by MHA supplement. However, protein carbonyl content and glutathione-S-transferase activity in head kidney, GSH content in spleen remained unaffected. After 60-day feeding trial, a challenge study was conducted by injection of Aeromonas hydrophila for 17 days. Results showed that survival rate, leukocytes phagocytic activity, lysozyme activity, acid phosphatase activity, total iron-binding capacity, haemagglutination titre, complement 3, 4 and immunoglobulin M contents significantly increased by optimal dietary MHA supplement (P < 0.05). These data suggested that MHA affected antioxidant status of immune organs and promoted immune response in juvenile Jian carp.
authors.affiliation: Animal Nutrition Institute, Sichuan Agricultural University, Ya'an, China.
authors.forename: Sheng-Yao
authors.initials: SY
authors.lastname: Kuang
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Xiao
authors.forename: Lin
authors.initials: L
authors.lastname: Feng
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Jiang
authors.forename: Wei-Dan
authors.initials: WD
authors.lastname: Jiang
authors.forename: Kai
authors.initials: K
authors.lastname: Hu
authors.forename: Shu-Hong
authors.initials: SH
authors.lastname: Li
authors.forename: Ling
authors.initials: L
authors.lastname: Tang
authors.forename: Xiao-Qiu
authors.initials: XQ
authors.lastname: Zhou
mesh_terms: D016980:Aeromonas hydrophila / Q000276:immunology; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002347:Carps / Q000276:immunology* / Q000378:metabolism*; D019587:Dietary Supplements; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D005393:Fish Diseases / Q000276:immunology; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000662:veterinary; D060226:Head Kidney / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D007962:Leukocytes / Q000187:drug effects / Q000378:metabolism; D008715:Methionine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000378:metabolism; D010084:Oxidation-Reduction; D011897:Random Allocation; D013154:Spleen / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism
chemical_list: D000975:Antioxidants; C008391:alpha-hydroxy-gamma-methylmercaptobutyric acid; D008715:Methionine