title: Neural activity and neurotransmission regulate the maturation of the innervation field of cortical GABAergic interneurons in an age-dependent manner.
abstract: Neural activity guides the patterning of neuron synaptic territory in the developing nervous system. Evidence supporting this hypothesis comes from numerous studies on projection neurons in neuromuscular and visual systems. It is unknown whether the innervation field of GABAergic interneurons, which forms local dense innervations, follows similar rules. Cortical basket cells innervate hundreds of pyramidal cell somata and proximal dendrites. Thanks to this connectivity pattern, they can tightly control neural excitability and synchronization. Here we show that reducing excitation, and thus neurotransmitter release, in mouse cortical single basket cells in slice cultures decreases the number of innervated cells without changing the pattern of perisomatic innervation, both at the peak and after the proliferation phase of perisomatic synapse formation. Conversely, suppressing neurotransmitter release in single basket cells can have completely opposite effects depending on the developmental stage. Our results reveal a remarkably specific and age-dependent role of neural activity and neurotransmission levels in the establishment of the synaptic territory of cortical GABAergic cells.
authors.affiliation: Centre Hospitalier Universitaire Sainte-Justine and Université de Montréal, Montréal, Quebec H3T 1C5, Canada.
authors.forename: Elie
authors.initials: E
authors.lastname: Baho
authors.forename: Graziella
authors.initials: G
authors.lastname: Di Cristo
mesh_terms: D000375:Aging / Q000235:genetics / Q000502:physiology*; D000704:Analysis of Variance; D000818:Animals; D001369:Axons / Q000187:drug effects / Q000378:metabolism; D050882:CREB-Binding Protein / Q000378:metabolism; D002540:Cerebral Cortex / Q000166:cytology* / Q000187:drug effects; D005260:Female; D059330:GABAergic Neurons / Q000187:drug effects / Q000502:physiology*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000235:genetics; D005968:Glutamate Decarboxylase / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics; D007395:Interneurons / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008666:Metalloendopeptidases / Q000494:pharmacology; D051379:Mice; D008822:Mice, Transgenic; D009433:Neural Inhibition / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D016501:Neurites / Q000187:drug effects / Q000378:metabolism; D009479:Neuropeptides / Q000737:chemistry / Q000378:metabolism; D009924:Organ Culture Techniques; D010455:Peptides / Q000494:pharmacology; D018013:Receptors, Neuropeptide / Q000235:genetics; D013569:Synapses / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D013744:Tetanus Toxin / Q000494:pharmacology; D014162:Transfection
chemical_list: D009479:Neuropeptides; D010455:Peptides; D018013:Receptors, Neuropeptide; D013744:Tetanus Toxin; C509142:allatostatin receptor, mouse; C076523:allatostatin; D049452:Green Fluorescent Proteins; D050882:CREB-Binding Protein; D008666:Metalloendopeptidases; C093556:zinc-endopeptidase, tetanus neurotoxin; D005968:Glutamate Decarboxylase; C401141:glutamate decarboxylase 2