title: Characterization of a new ScbR-like γ-butyrolactone binding regulator (SlbR) in Streptomyces coelicolor.
abstract: γ-Butyrolactones in Streptomyces are well recognized as bacterial hormones, and they affect secondary metabolism of Streptomyces. γ-Butyrolactone receptors are considered important regulatory proteins, and various γ-butyrolactone synthases and receptors have been reported in Streptomyces. Here, we characterized a new regulator, SCO0608, that interacted with SCB1 (γ-butyrolactone of Streptomyces coelicolor) and bound to the scbR/A and adpA promoters. The SCO0608 protein sequences are not similar to those of any known γ-butyrolactone binding proteins in Streptomyces such as ScbR from S. coelicolor or ArpA from Streptomyces griseus. Interestingly, SCO0608 functions as a repressor of antibiotic biosynthesis and spore formation in R5 complex media. We showed the existence of another type of γ-butyrolactone receptor in Streptomyces, and this SCO0608 was named ScbR-like γ-butyrolactone binding regulator (SlbR) in S. coelicolor.
authors.affiliation: Department of Microbial Engineering, College of Engineering, Konkuk University, Seoul, South Korea.
authors.forename: Yung-Hun
authors.initials: YH
authors.lastname: Yang
authors.forename: Eunjung
authors.initials: E
authors.lastname: Song
authors.forename: Ji-Nu
authors.initials: JN
authors.lastname: Kim
authors.forename: Bo-Rahm
authors.initials: BR
authors.lastname: Lee
authors.forename: Eun-Jung
authors.initials: EJ
authors.lastname: Kim
authors.forename: Sung-Hee
authors.initials: SH
authors.lastname: Park
authors.forename: Woo-Seong
authors.initials: WS
authors.lastname: Kim
authors.forename: Hyung-Yeon
authors.initials: HY
authors.lastname: Park
authors.forename: Jong-Min
authors.initials: JM
authors.lastname: Jeon
authors.forename: Thangamani
authors.initials: T
authors.lastname: Rajesh
authors.forename: Yun-Gon
authors.initials: YG
authors.lastname: Kim
authors.forename: Byung-Gee
authors.initials: BG
authors.lastname: Kim
mesh_terms: D015107:4-Butyrolactone / Q000378:metabolism; D004269:DNA, Bacterial / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D025941:Protein Interaction Mapping; D011963:Receptors, GABA-A / Q000235:genetics* / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D048372:Streptomyces coelicolor / Q000235:genetics* / Q000378:metabolism*; D013305:Streptomyces griseus / Q000235:genetics
chemical_list: C037481:4-butyrolactone receptor; D004269:DNA, Bacterial; D011963:Receptors, GABA-A; D012097:Repressor Proteins; D015107:4-Butyrolactone