title: MicroRNAs control neurobehavioral development and function in zebrafish.
abstract: microRNAs (miRNAs) have emerged as regulators of a broad spectrum of neurodevelopmental processes, including brain morphogenesis, neuronal differentiation, and survival. While the role of miRNAs in establishing and maintaining the developing nervous system is widely appreciated, the developmental neurobehavioral role of miRNAs has yet to be defined. Here we show that transient disruption of brain morphogenesis by ethanol exposure results in behavioral hyperactivity in larval zebrafish challenged with changes in lighting conditions. Aberrations in swimming activity persist in juveniles that were developmentally exposed to ethanol. During early neurogenesis, multiple gene expression profiling studies revealed widespread changes in mRNA and miRNA abundance in ethanol-exposed embryos. Consistent with a role for miRNAs in neurobehavioral development, target prediction analyses identified multiple miRNAs misexpressed in the ethanol-exposed cohorts that were also predicted to target inversely expressed transcripts known to influence brain morphogenesis. In vivo knockdown of miR-9/9* or miR-153c persistently phenocopied the effect of ethanol on larval and juvenile swimming behavior. Structural analyses performed on adults showed that repression of miR-153c during development impacts craniofacial skeletal development. Together, these data support an integral role for miRNAs in the establishment of vertebrate neurobehavioral and skeletal systems.
authors.affiliation: Department of Environmental and Molecular Toxicology, Environmental Health Sciences Center, Oregon State University, 28645 East HWY 34, Corvallis, OR 97333, USA.
authors.forename: Tamara L
authors.initials: TL
authors.lastname: Tal
authors.forename: Jill A
authors.initials: JA
authors.lastname: Franzosa
authors.forename: Susan C
authors.initials: SC
authors.lastname: Tilton
authors.forename: Kenneth A
authors.initials: KA
authors.lastname: Philbrick
authors.forename: Urszula T
authors.initials: UT
authors.lastname: Iwaniec
authors.forename: Russell T
authors.initials: RT
authors.lastname: Turner
authors.forename: Katrina M
authors.initials: KM
authors.lastname: Waters
authors.forename: Robert L
authors.initials: RL
authors.lastname: Tanguay
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects / Q000502:physiology*; D001921:Brain / Q000187:drug effects / Q000196:embryology* / Q000254:growth & development*; D004625:Embryo, Nonmammalian / Q000033:anatomy & histology / Q000187:drug effects / Q000502:physiology; D000431:Ethanol / Q000494:pharmacology; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D055785:Gene Knockdown Techniques; D006801:Humans; D007814:Larva / Q000033:anatomy & histology / Q000502:physiology; D008027:Light; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D038081:Organogenesis / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D015027:Zebrafish / Q000033:anatomy & histology / Q000235:genetics / Q000502:physiology*
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger; D000431:Ethanol