title: Cell interaction study of amyloid by using luminescent conjugated polythiophene: implication that amyloid cytotoxicity is correlated with prolonged cellular binding.
abstract: Needles and noodles: Studying amyloid toxicity is important for understanding protein misfolding diseases. Using a luminescent conjugated polythiophene, we found that cell binding of nontoxic filamentous amyloids of insulin and β2-microglobulin was less efficient than that of toxic fibrillar amyloids; this suggests a correlation between amyloid toxicity and cell binding.
authors.affiliation: Bioengineering Laboratory, RIKEN Institute, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan. zako@riken.jp
authors.forename: Tamotsu
authors.initials: T
authors.lastname: Zako
authors.forename: Masafumi
authors.initials: M
authors.lastname: Sakono
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Karin
authors.initials: K
authors.lastname: Sörgjerd
authors.forename: K Peter R
authors.initials: KP
authors.lastname: Nilsson
authors.forename: Per
authors.initials: P
authors.lastname: Hammarström
authors.forename: Mikael
authors.initials: M
authors.lastname: Lindgren
authors.forename: Mizuo
authors.initials: M
authors.lastname: Maeda
mesh_terms: D000682:Amyloid / Q000737:chemistry* / Q000494:pharmacology*; D000818:Animals; D001665:Binding Sites / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D057809:HEK293 Cells; D006801:Humans; D049449:Luminescence; D016716:PC12 Cells; D011108:Polymers / Q000737:chemistry*; D051381:Rats; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship; D013876:Thiophenes / Q000737:chemistry*
chemical_list: D000682:Amyloid; D011108:Polymers; D013876:Thiophenes; C066730:polythiophene