title: Antimicrobial susceptibility and molecular characteristics of 857 methicillin-resistant Staphylococcus aureus isolates from 16 medical centers in Japan (2008-2009): nationwide survey of community-acquired and nosocomial MRSA.
abstract: This study is a nationwide survey of all clinical methicillin-resistant Staphylococcus aureus (MRSA) isolates, including community-acquired MRSA (CA-MRSA), in Japan. A total of 857 MRSA clinical isolates were collected from the 16 institutions throughout Japan that participated in the survey (2008-2009). The drug susceptibility and staphylococcal cassette chromosome mec (SCCmec) typing and the presence of specific pathogenic genes were evaluated. The isolates comprised SCCmec type II (73.6%), type IV (20%), and type I (6%). The percentage of SCCmec type IV isolates was significantly higher in outpatients than in inpatients. Most of the isolated strains were sensitive to vancomycin (VCM, MIC ≤2 μg/mL), linezolid (MIC ≤4 μg/mL), and teicoplanin (MIC ≤8 μg/mL). Although most strains were sensitive to VCM, the MIC value of VCM for SCCmec type II strains was higher than that for SCCmec type IV strains. Only 4 (2.3%) of 171 SCCmec type IV strains were Panton-Valentine leukocidin (lukS/F-PV)-positive. Thus, this result indicates a unique feature of SCCmec type IV strains in Japan. The information in this study not only is important in terms of local public health but will also contribute to an understanding of epidemic clones of CA-MRSA.
authors.affiliation: Nagasaki University Hospital, 852-8501, Japan. k-yanagi@nagasaki-u.ac.jp
authors.forename: Katsunori
authors.initials: K
authors.lastname: Yanagihara
authors.forename: Nobuko
authors.initials: N
authors.lastname: Araki
authors.forename: Shinichi
authors.initials: S
authors.lastname: Watanabe
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kinebuchi
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Kaku
authors.forename: Shigefumi
authors.initials: S
authors.lastname: Maesaki
authors.forename: Keizo
authors.initials: K
authors.lastname: Yamaguchi
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Matsumoto
authors.forename: Hiroshige
authors.initials: H
authors.lastname: Mikamo
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Takesue
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Kadota
authors.forename: Jiro
authors.initials: J
authors.lastname: Fujita
authors.forename: Keiji
authors.initials: K
authors.lastname: Iwatsuki
authors.forename: Haruko
authors.initials: H
authors.lastname: Hino
authors.forename: Takehiko
authors.initials: T
authors.lastname: Kaneko
authors.forename: Kenji
authors.initials: K
authors.lastname: Asagoe
authors.forename: Masami
authors.initials: M
authors.lastname: Ikeda
authors.forename: Akira
authors.initials: A
authors.lastname: Yasuoka
authors.forename: Shigeru
authors.initials: S
authors.lastname: Kohno
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000890:Anti-Infective Agents / Q000494:pharmacology*; D002648:Child; D002675:Child, Preschool; D017714:Community-Acquired Infections / Q000453:epidemiology / Q000382:microbiology*; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology*; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007564:Japan / Q000453:epidemiology; D008297:Male; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D058889:Molecular Typing; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology*; D037521:Virulence Factors / Q000235:genetics; D055815:Young Adult
chemical_list: D000890:Anti-Infective Agents; D037521:Virulence Factors