title: Altered miRNA expression patterns in Tff2 knock-out mice correlate with             cellular pathways of neoplastic development and caloric metabolism.
abstract: The trefoil peptide family, consisting in mammals of three members namely TFF1, 2 and 3, plays a cytoprotective role in epithelial cells of various tissues, mainly in the digestive tract. Tff1, Tff2 or Tff3 knock-out mouse models developed various kinds of gastrointestinal impairment. microRNAs are known to be novel gene regulators. We aimed to investigate the physiological role of such miRNAs in Tff2 knock-out mice. Whole miRNome profiling and in silico analysis were performed for Tff2-KO and WT mice. Our latest data explored the role of miRNAs in the regulatory cascades and molecular processes of Tff2-/- mice. As much as 6% of the Tff2-KO mice miRNome was significantly dys-regulated. Further in silico analysis suggests that the respective dys-regulated part of the miRNome is involved in human pathological processes, including pancreatic, colorectal and basal cell cancer. Additionally, the dys-regulated miRNome targets pathways involved in carbohydrate metabolism and adipocytokine signaling. The latter links deficient caloric maintenance in Tff2 and previous observation in Tff3-KO mice with miRNAs. In summary, our proof-of-concept study indicates that miRNAs may play an important role in the regulatory processes of the trefoil peptide family, especially in the regulation of cancer-related cascades.
authors.affiliation: Division of Molecular Genetics, Institute of Human Genetics, University of Tübingen, Wilhelmstr. 27, D-72074 Tübingen, Germany. aftabalishah1@gmail.com
authors.forename: Aftab Ali
authors.initials: AA
authors.lastname: Shah
authors.forename: Petra
authors.initials: P
authors.lastname: Leidinger
authors.forename: Andreas
authors.initials: A
authors.lastname: Keller
authors.forename: Anke
authors.initials: A
authors.lastname: Wendschlag
authors.forename: Eckart
authors.initials: E
authors.lastname: Meese
authors.forename: Nikolaus
authors.initials: N
authors.lastname: Blin
mesh_terms: D054392:Adipokines / Q000235:genetics / Q000378:metabolism; D000818:Animals; D050260:Carbohydrate Metabolism / Q000235:genetics; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D005809:Genes, Regulator; D051379:Mice; D018345:Mice, Knockout; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009077:Mucins / Q000235:genetics / Q000378:metabolism*; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism*; D010455:Peptides / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D000071164:Trefoil Factor-2
chemical_list: D054392:Adipokines; D035683:MicroRNAs; D009077:Mucins; D009124:Muscle Proteins; D010455:Peptides; C000604007:TFF2 protein, human; C481546:TFF2 protein, mouse; D000071164:Trefoil Factor-2