title: Comprehensive two-dimensional gas chromatography in metabolomics.
abstract: One of the major objectives in metabolomics is the identification of subtle changes in metabolite profiles as affected by genetic or environmental factors. Comprehensive two-dimensional gas chromatography (GC × GC) hyphenated to a fast-acquisition mass spectrometer is a well-established analytical technique to study the composition of complex samples due to its enhanced separation capacity, sensitivity, peak resolution, and reproducibility. This review reports applications of GC × GC to metabolomics studies of sample of different types (biofluid, cells, tissue, bacteria, yeast, plants), and discusses its advantages and limitations.
authors.affiliation: Institute of Functional Genomics, University of Regensburg, Regensburg, Germany.
authors.forename: Martin F
authors.initials: MF
authors.lastname: Almstetter
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Oefner
authors.forename: Katja
authors.initials: K
authors.lastname: Dettmer
mesh_terms: D000818:Animals; D001419:Bacteria / Q000378:metabolism; D002849:Chromatography, Gas / Q000379:methods*; D006801:Humans; D055442:Metabolome; D055432:Metabolomics / Q000379:methods*; D010944:Plants / Q000378:metabolism; D015003:Yeasts / Q000378:metabolism