title: Time-course evaluation of oxidative stress-related biomarkers after renal transplantation.
abstract: Patients with chronic renal disease have a high prevalence of oxidative stress (OS), which is associated with the cardiovascular complications occurring in this population. The restoration of kidney function after kidney transplantation (KT) can lead to reduction in the metabolic abnormalities and elimination of the OS. Time-dependent changes in OS-related markers and specific kidney function and metabolic parameters were evaluated in patients (N = 39; 23 males; 16 females; mean age = 57 ± 10 years) before (day 0) and after KT (day 1, 7, 30, 90, and 180) to monitor the graft. In particular, total antioxidant capacity (TAC), levels of advanced oxidation protein products (AOPP), lipid peroxidation as thiobarbituric acid-reactive substances (TBARS) and reduced glutathione (GSH); activities of glutathione peroxidase, catalase, and superoxide dismutase; and kidney function markers were measured. AOPP, TAC, and TBARS were significantly decreased, whereas GSH was significantly increased after KT. Antioxidant enzyme activities were not significantly changed during the monitored period after KT. Apropos specific kidney function markers and glomerular filtration significantly increased and creatinine level significantly decreased after transplantation. Changes in high-density lipoprotein cholesterol were also found. Our results show that successful KT results in normalization of the antioxidant status and lipid metabolism that is connected with both improved renal function and reduced cardiovascular complications.
authors.affiliation: Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacký University, Olomouc, Czech Republic.
authors.forename: Jitka
authors.initials: J
authors.lastname: Vostálová
authors.forename: Adéla
authors.initials: A
authors.lastname: Galandáková
authors.forename: Alena Rajnochová
authors.initials: AR
authors.lastname: Svobodová
authors.forename: Eva
authors.initials: E
authors.lastname: Orolinová
authors.forename: Markéta
authors.initials: M
authors.lastname: Kajabová
authors.forename: Petr
authors.initials: P
authors.lastname: Schneiderka
authors.forename: Jana
authors.initials: J
authors.lastname: Zapletalová
authors.forename: Pavel
authors.initials: P
authors.lastname: Strebl
authors.forename: Josef
authors.initials: J
authors.lastname: Zadražil
mesh_terms: D015415:Biomarkers / Q000097:blood*; D005260:Female; D005500:Follow-Up Studies; D005978:Glutathione / Q000097:blood*; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood / Q000601:surgery*; D007677:Kidney Function Tests; D016030:Kidney Transplantation / Q000502:physiology*; D015227:Lipid Peroxidation; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D011379:Prognosis; D011446:Prospective Studies; D013482:Superoxide Dismutase / Q000097:blood*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism*; D013997:Time Factors
chemical_list: D015415:Biomarkers; D017392:Thiobarbituric Acid Reactive Substances; D013482:Superoxide Dismutase; D005978:Glutathione