title: Evaluation of the single yeast cell's adhesion to ITO substrates with various surface energies via ESEM nanorobotic manipulation system.
abstract: Cell-surface adhesion force is important for cell activities and the development of bio materials. In this paper, a method for in situ single cell   (W303) adhesion force measurement was proposed based on nanorobotic manipulation system inside an environment scanning electron microscope (ESEM). An end effector was fabricated from a commercial atomic force microscope (AFM) cantilever by focused ion beam (FIB) etching. The spring constant of it was calibrated by nanomanipulation approach. Three kinds of hydrophilic and hydrophobic ITO plates were prepared by using VUV-irradiation and OTS coating techniques. The shear adhesion strength of the single yeast cell to each substrate was measured based on the deflection of the end effector. The results demonstrated that the cell adhesion force was larger under the wet condition in the ESEM environment than in the aqueous condition. It also showed that the cell adhesion force to hydrophilic surface was larger than that to the hydrophobic surface. Studies of single cell's adhesion on various plate surfaces and environments could give new insights into the tissue engineering and biological field.
authors.affiliation: Department of Micro-Nano Systems Engineering, Nagoya University, Nagoya, Japan. shen@robo.mein.nagoya-u.ac.jp
authors.forename: Yajing
authors.initials: Y
authors.lastname: Shen
authors.forename: Mohd Ridzuan
authors.initials: MR
authors.lastname: Ahmad
authors.forename: Masahiro
authors.initials: M
authors.lastname: Nakajima
authors.forename: Seiji
authors.initials: S
authors.lastname: Kojima
authors.forename: Michio
authors.initials: M
authors.lastname: Homma
authors.forename: Toshio
authors.initials: T
authors.lastname: Fukuda
mesh_terms: D001672:Biocompatible Materials; D002138:Calibration; D002448:Cell Adhesion; D017276:Friction; D005898:Glass; D057927:Hydrophobic and Hydrophilic Interactions; D008855:Microscopy, Electron, Scanning / Q000379:methods; D008954:Models, Biological; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D012371:Robotics / Q000295:instrumentation / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure*; D059010:Single-Cell Analysis; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials