title: Restriction modification (RM) tests associated to additional molecular markers for screening prevalent MRSA clones in Brazil.
abstract: In this study, we associated the restriction modification (RM) tests to the polymerase chain reaction (PCR) detection of molecular markers (SCCmec III, seh, agr II-SCCmec IV, and lukSF) for revealing the main methicillin-resistant Staphylococcus aureus (MRSA) clones circulating in Brazil. This simple and rapid approach allowed a precise classification of the MRSA analyzed when compared with pulsed-field gel electrophoresis (PFGE) data.
authors.affiliation: Instituto de Microbiologia Paulo de Góes, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Av. Carlos Chagas Filho 373, Cidade Universitária, Rio de Janeiro, RJ, 21941-902, Brazil.
authors.forename: C O
authors.initials: CO
authors.lastname: Beltrame
authors.forename: A M N
authors.initials: AM
authors.lastname: Botelho
authors.forename: M C
authors.initials: MC
authors.lastname: Silva-Carvalho
authors.forename: R R
authors.initials: RR
authors.lastname: Souza
authors.forename: R R
authors.initials: RR
authors.lastname: Bonelli
authors.forename: M S
authors.initials: MS
authors.lastname: Ramundo
authors.forename: M A
authors.initials: MA
authors.lastname: Guimarães
authors.forename: L R
authors.initials: LR
authors.lastname: Coelho
authors.forename: A M S
authors.initials: AM
authors.lastname: Figueiredo
mesh_terms: D001938:Brazil; D016000:Cluster Analysis; D015280:DNA Restriction-Modification Enzymes; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D058889:Molecular Typing / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013203:Staphylococcal Infections / Q000382:microbiology*
chemical_list: D015280:DNA Restriction-Modification Enzymes