title: Development and optimization of a fluorescent differential display PCR system for analyzing the stress response in Lactobacillus sakei strains.
abstract: Lactobacillus sakei is widely used as starter in the production process of Italian fermented sausages and its growth and survival are affected by various factors. We studied the differential expression of genome in response to different stresses by the fluorescent differential display (FDD) technique. This study resulted in the development and optimization of an innovative technique, with a high level of reproducibility and quality, which allows the identification of gene expression changes associated with different microbial behaviours under different growth conditions.
authors.affiliation: Dipartimento di Biologia, Difesa e Biotecnologie Agro-Forestali, Università degli Studi della Basilicata, Viale dell'Ateneo Lucano, 10, 85100 Potenza, Italy. mariaanna.sico@unibas.it
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Bonomo
authors.forename: Maria Anna
authors.initials: MA
authors.lastname: Sico
authors.forename: Simona
authors.initials: S
authors.lastname: Grieco
authors.forename: Giovanni
authors.initials: G
authors.lastname: Salzano
mesh_terms: D005453:Fluorescence; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D007778:Lactobacillus / Q000502:physiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013312:Stress, Physiological