title: Luria-delbruck estimation of turnip mosaic virus mutation rate in vivo.
abstract: A potential drawback of recent antiviral therapies based on the transgenic expression of artificial microRNAs is the ease with which viruses may generate escape mutations. Using a variation of the classic Luria-Delbrück fluctuation assay, we estimated that the spontaneous mutation rate in the artificial microRNA (amiR) target of a plant virus was ca. 6 × 10(-5) per replication event.
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas-UPV, València, Spain.
authors.forename: Francisca
authors.initials: F
authors.lastname: de la Iglesia
authors.forename: Fernando
authors.initials: F
authors.lastname: Martínez
authors.forename: Julia
authors.initials: J
authors.lastname: Hillung
authors.forename: José M
authors.initials: JM
authors.lastname: Cuevas
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Gerrish
authors.forename: José-Antonio
authors.initials: JA
authors.lastname: Daròs
authors.forename: Santiago F
authors.initials: SF
authors.lastname: Elena
mesh_terms: D008957:Models, Genetic; D009029:Mosaic Viruses / Q000235:genetics*; D009154:Mutation; D059645:Mutation Rate; D010935:Plant Diseases / Q000821:virology