title: Time course of prion seeding activity in cerebrospinal fluid of scrapie-infected hamsters after intratongue and intracerebral inoculations.
abstract: To assess prospects for early diagnosis of prion disease based on prion seeding activity in cerebrospinal fluid (CSF), we measured the activity over time in scrapie-infected hamsters by real-time quaking-induced conversion (RT-QuIC). After intracerebral inoculation, activity appeared in CSF within 1 day and plateaued weeks before the onset of clinical signs. However, after intratongue inoculation, activity first appeared in CSF with the onset of clinical signs, well after higher-level accumulation of seeding activity in brain.
authors.affiliation: Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA.
authors.forename: Christina D
authors.initials: CD
authors.lastname: Orrù
authors.forename: Andrew G
authors.initials: AG
authors.lastname: Hughson
authors.forename: Brent
authors.initials: B
authors.lastname: Race
authors.forename: Gregory J
authors.initials: GJ
authors.lastname: Raymond
authors.forename: Byron
authors.initials: B
authors.lastname: Caughey
mesh_terms: D000682:Amyloid / Q000134:cerebrospinal fluid* / Q000737:chemistry; D000818:Animals; D001921:Brain / Q000473:pathology; D006224:Cricetinae; D007700:Kinetics; D057230:Limit of Detection; D011328:Prions / Q000134:cerebrospinal fluid* / Q000737:chemistry; D012608:Scrapie / Q000134:cerebrospinal fluid* / Q000175:diagnosis; D014059:Tongue / Q000473:pathology
chemical_list: D000682:Amyloid; D011328:Prions