title: Monomeric α-synuclein binds Congo Red micelles in a disordered manner.
abstract: The histological dye Congo Red (CR) previously has been shown to inhibit α-synuclein (aS) fibrillation, but the mode of this inhibition remained unclear. Because of favorable exchange kinetics, interaction between CR and aS lends itself to a detailed nuclear magnetic resonance study, and relaxation dispersion measurements yield the bound fraction and time scales for the interaction of aS with CR. We find that at pH 6, CR exists as a micelle, and at a CR:aS molar ratio of ~1, only a small fraction of aS (~2%) is bound to these micelles. Rapid exchange (k(ex) ~ 3000 s(-1)) between the free and CR-bound states broadens and strongly attenuates resonances of aS by two processes: a magnetic field-dependent contribution, caused by the chemical shift difference between the two states, and a nearly field-independent contribution caused by slower tumbling of aS bound to the CR micelle. The salt dependence of the interaction suggests a predominantly electrostatic mechanism for the 60 N-terminal residues, while the weaker interaction between residues 61-100 and CR is mostly hydrophobic. Chemical shift and transferred NOE data indicate that aS becomes slightly more helical but remains largely disordered when bound to CR. Results indicate that inhibition of fibril formation does not result from binding of CR to free aS and, therefore, must result from interaction of aS fibrils or protofibrils with CR micelles.
authors.affiliation: Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892-0520, United States.
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Maltsev
authors.forename: Alexander
authors.initials: A
authors.lastname: Grishaev
authors.forename: Ad
authors.initials: A
authors.lastname: Bax
mesh_terms: D004396:Coloring Agents / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D003224:Congo Red / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008027:Light; D008823:Micelles; D019906:Nuclear Magnetic Resonance, Biomolecular; D009994:Osmolar Concentration; D011485:Protein Binding; D017433:Protein Structure, Secondary / Q000187:drug effects; D053838:Scattering, Small Angle; D014867:Water / Q000737:chemistry; D014961:X-Ray Diffraction; D051844:alpha-Synuclein / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D004396:Coloring Agents; D008823:Micelles; D051844:alpha-Synuclein; D014867:Water; D003224:Congo Red