title: [Combination of bla(VIM-1) and qnrS2 on the same plasmid in Klebsiella oxytoca and Klebsiella pneumoniae isolates in Seville].
abstract: BACKGROUND
  Combined resistance to quinolones and β-lactams is common in Enterobacteriaceae. The appearance in enterobacteria coding for metallo-β-lactamases and determinants of plasmid-mediated quinolone resistance are an emerging problem in our country.
  
  
  METHODS
  The susceptibility was determined by E-test. The resistance genes were detected by PCR and the corresponding plasmids were characterised.
  
  
  RESULTS
  This study describes 2 strains (1 Klebsiella oxytoca, 1 Klebsiella pneumoniae) carrying the genes qnrS2 and blaVIM-1 in a transferable plasmid of 70-Kb isolated in surveillance cultures at the University Hospital Virgen Macarena in Seville.
  
  
  CONCLUSIONS
  This is the first combination of qnrS2 and bla(VIM-1) on the same non-typeable plasmid isolated in our centre.
authors.affiliation: Departamento de Microbiología, Facultad de Medicina, Universidad de Sevilla, Sevilla, España. jmrodriguez@us.es
authors.forename: José M
authors.initials: JM
authors.lastname: Rodríguez-Martínez
authors.forename: M Carmen
authors.initials: MC
authors.lastname: Conejo
authors.forename: Paula
authors.initials: P
authors.lastname: Díaz de Alba
authors.forename: Lorena
authors.initials: L
authors.lastname: López-Cerero
authors.forename: Pedro
authors.initials: P
authors.lastname: Fernandez-Echauri
authors.forename: Alvaro
authors.initials: A
authors.lastname: Pascual
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D006801:Humans; D041121:Klebsiella oxytoca / Q000187:drug effects* / Q000235:genetics*; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000235:genetics*; D010957:Plasmids / Q000235:genetics*; D013030:Spain; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; C485385:Qnr protein, Klebsiella pneumoniae; C417264:VIM-1 metallo-beta-lactamase; D001618:beta-Lactamases