title: Changes in T lymphocyte subsets and intracellular cytokines after transfer             of chemically extracted acellular nerve allografts.
abstract: The aim of the present study was to observe the immune mechanism underlying             the rejection of chemically extracted acellular nerve allografts for use in clinical             applications. A total of 128 BALB/c mice were randomly divided into a negative             contrast group (NC, 32 mice), a fresh autograft group (AG, 32 mice), a fresh allogeneic             nerve group (FN, 32 mice) and a chemically extracted acellular allogeneic nerve             group (CEN, 32 mice). Various types of nerve grafts were implanted into the thigh             muscle of BALB/C mice in the corresponding groups. At 3, 7, 14 and 28 days post-operation,             the mice (8 cases from each group) were sacrificed and their spleens were extracted.             The spleens were ground into paste. The erythrocytes and other cells were lysed             using distilled water and the T lymphocytes were collected. Monoclonal antibodies             (CD3, CD4, CD8, CD25, IL-2, IFN-γ and TNF-α) were then added to the solution.             The Facial Action Coding System was used to determine the positive rates of the             cells combined with the monoclonal antibodies above. No significant statistical             differences were observed between the CEN, NC and AG groups. However, some data             of the FN group were significantly higher than those of the other groups at the             corresponding time. No obvious immune rejections were observed among the chemically             extracted acellular nerve allografts compared with fresh nerve autograft.
authors.affiliation: Department of Orthopaedics, Navy General Hospital, Beijing 100037, PR China. weilichn@126.com
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Wen-Wen
authors.initials: WW
authors.lastname: Wu
authors.forename: Xing-Shi
authors.initials: XS
authors.lastname: Lin
authors.forename: Shu-Xun
authors.initials: SX
authors.lastname: Hou
authors.forename: Hong-Bin
authors.initials: HB
authors.lastname: Zhong
authors.forename: Di-Ke
authors.initials: DK
authors.lastname: Ruan
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002469:Cell Separation; D016207:Cytokines / Q000378:metabolism*; D007371:Interferon-gamma / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D012584:Sciatic Nerve / Q000166:cytology / Q000276:immunology / Q000637:transplantation*; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism*; D014182:Transplantation, Autologous; D014184:Transplantation, Homologous; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D016207:Cytokines; D007376:Interleukin-2; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma