title: Serial two-photon tomography for automated ex vivo mouse brain imaging.
abstract: Here we describe an automated method, named serial two-photon (STP) tomography, that achieves high-throughput fluorescence imaging of mouse brains by integrating two-photon microscopy and tissue sectioning. STP tomography generates high-resolution datasets that are free of distortions and can be readily warped in three dimensions, for example, for comparing multiple anatomical tracings. This method opens the door to routine systematic studies of neuroanatomy in mouse models of human brain disorders.
authors.affiliation: TissueVision, Inc., Cambridge, Massachusetts, USA.
authors.forename: Timothy
authors.initials: T
authors.lastname: Ragan
authors.forename: Lolahon R
authors.initials: LR
authors.lastname: Kadiri
authors.forename: Kannan Umadevi
authors.initials: KU
authors.lastname: Venkataraju
authors.forename: Karsten
authors.initials: K
authors.lastname: Bahlmann
authors.forename: Jason
authors.initials: J
authors.lastname: Sutin
authors.forename: Julian
authors.initials: J
authors.lastname: Taranda
authors.forename: Ignacio
authors.initials: I
authors.lastname: Arganda-Carreras
authors.forename: Yongsoo
authors.initials: Y
authors.lastname: Kim
authors.forename: H Sebastian
authors.initials: HS
authors.lastname: Seung
authors.forename: Pavel
authors.initials: P
authors.lastname: Osten
mesh_terms: D019412:Anatomy, Cross-Sectional / Q000379:methods*; D000818:Animals; D001921:Brain / Q000166:cytology*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D051379:Mice; D008822:Mice, Transgenic; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D010363:Pattern Recognition, Automated / Q000379:methods*; D014054:Tomography / Q000379:methods*