title: Distribution and chemical coding pattern of the cocaine- and amphetamine-regulated transcript (CART) immunoreactivity in the preoptic area of the pig.
abstract: This study provides a detailed description of cocaine-and amphetamine-regulated transcript (CART) distribution and the co-localization pattern of CART and gonadotropin releasing hormone (GnRH), somatostatin (SOM), neuropeptide Y (NPY), cholecystokinin (CCK), and substance P (SP) in the preoptic area (POA) of the domestic pig. The POA displays a low density of immunoreactive cells and rich immunoreactivity for CART in fibers. CART-immunoreactive (CART-IR) cell bodies were single and faintly stained, and located in the medial preoptic area (MPA) and the periventricular region of the POA. A high density of immunoreactive fibers was observed in the periventricular preoptic nucleus (PPN); a high to moderate density of fibers was observed in the MPA; but in the dorso-medial region of the MPA the highest density of fibers in the whole POA was observed. The lateral preoptic area (LPA) exhibited a less dense concentration of CART-immunoreactive fibers than the MPA. The median preoptic nucleus (MPN) showed moderate to low expression of staining fibers. In the present study, dual-labeling immunohistochemistry was used to show that CART-IR cell bodies do not contain any GnRH and SP. CART-positive fibers were identified in close apposition with GnRH neurons. This suggests that CART may influence GnRH secretion. Double staining revealed that CART-IR structures do not co-express any of the substances we studied, but a very small population of CART-IR fibers also contain SOM, CCK or SP.
authors.affiliation: Department of Comparative Anatomy, Faculty of Biology, University of Warmia and Mazury in Olsztyn, Poland. boguska@uwm.edu.pl
authors.forename: Krystyna
authors.initials: K
authors.lastname: Bogus-Nowakowska
authors.forename: Anna
authors.initials: A
authors.lastname: Robak
authors.forename: Maciej
authors.initials: M
authors.lastname: Równiak
authors.forename: Barbara
authors.initials: B
authors.lastname: Wasilewska
authors.forename: Janusz
authors.initials: J
authors.lastname: Najdzion
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Kolenkiewicz
authors.forename: Witold
authors.initials: W
authors.lastname: Zakowski
authors.forename: Mariusz
authors.initials: M
authors.lastname: Majewski
mesh_terms: D000661:Amphetamine / Q000494:pharmacology*; D000818:Animals; D002766:Cholecystokinin / Q000378:metabolism; D003042:Cocaine / Q000378:metabolism / Q000494:pharmacology*; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism; D007150:Immunohistochemistry; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D009478:Neuropeptide Y / Q000378:metabolism; D011301:Preoptic Area / Q000166:cytology / Q000378:metabolism*; D013004:Somatostatin / Q000378:metabolism; D013373:Substance P / Q000378:metabolism; D034421:Sus scrofa; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein
chemical_list: D009419:Nerve Tissue Proteins; D009478:Neuropeptide Y; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein; D013373:Substance P; D007987:Gonadotropin-Releasing Hormone; D013004:Somatostatin; D002766:Cholecystokinin; D000661:Amphetamine; D003042:Cocaine