title: Identification of goat mammary stem/progenitor cells.
abstract: Goat mammary gland epithelial cells have been used to establish primary and permanent cell lines, but to date, no data have been available regarding mammary stem cells (MaSCs) in this species. The detection and characterization of goat MaSCs is an important task for a better understanding of the cyclic character of mammary gland development, which will also offer the potential for manipulation of lactation yield and persistency. The objective of the present study was to demonstrate that a subpopulation of goat MaSCs resides in the goat mammary gland. Mammary tissue from lactating Saanen goats (Capra hircus) was dissociated and processed to a single-cell suspension. Using an in vitro colony-forming assay, we demonstrated that distinct colony types, which expressed specific lineage markers, arose from unipotent progenitors. Using two different growth media, we showed that the frequencies of caprine clonogenic progenitors differed according to growth conditions. Goat epithelial cells were transplanted under the kidney capsule of nonobese diabetic/severe combined immunodeficient (NOD/SCID) mice, where they formed organized, bilayered structures. Our results indicate the presence of goat MaSCs in the caprine mammary gland. To our knowledge, these data represent the first description of the tissue hierarchy of the goat mammary gland and demonstrate the regenerative potential of adult goat MaSCs.
authors.affiliation: Department of Animal Science, University of Ljubljana, Domzale, Slovenia.
authors.forename: Sonja
authors.initials: S
authors.lastname: Prpar
authors.forename: Eugenio
authors.initials: E
authors.lastname: Martignani
authors.forename: Peter
authors.initials: P
authors.lastname: Dovc
authors.forename: Mario
authors.initials: M
authors.lastname: Baratta
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002469:Cell Separation; D003114:Colony-Forming Units Assay; D004847:Epithelial Cells / Q000166:cytology*; D005260:Female; D006041:Goats; D007774:Lactation; D008321:Mammary Glands, Animal / Q000166:cytology*; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*