title: Identification of continuous epitopes of HuD antibodies related to paraneoplastic diseases/small cell lung cancer.
abstract: HuD antibodies are associated with small cell lung cancer. To identify relevant epitopes of HuD antibodies, patient sera and a monoclonal antibody were analyzed for their reactivity to linear 20mer peptides spanning the human HuD protein. The HuD monoclonal antibody recognized a single fragment located in the first RNA recognition motif. Thorough analysis identified VRDKITQGSL as the actual epitope. Screening of anti-HuD positive patients and healthy controls identified eight peptides as potential subdominant epitopes. The majority of these peptides were located in the N-terminal end as well as in the linker region between the second and third RNA recognition motifs.
authors.affiliation: Department of Clinical Biochemistry and Immunology, Statens Serum Institut, Ørestads Boulevard 5, 2300 Copenhagen S, Denmark.
authors.forename: Nicole Hartwig
authors.initials: NH
authors.lastname: Trier
authors.forename: Paul Robert
authors.initials: PR
authors.lastname: Hansen
authors.forename: Christian A
authors.initials: CA
authors.lastname: Vedeler
authors.forename: Finn Engeborg
authors.initials: FE
authors.lastname: Somnier
authors.forename: Gunnar
authors.initials: G
authors.lastname: Houen
mesh_terms: D000911:Antibodies, Monoclonal / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D051959:ELAV Proteins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D018604:Epitope Mapping; D000939:Epitopes / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood* / Q000276:immunology; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010257:Paraneoplastic Syndromes / Q000097:blood* / Q000276:immunology; D055752:Small Cell Lung Carcinoma / Q000097:blood* / Q000276:immunology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000911:Antibodies, Monoclonal; D051959:ELAV Proteins; D000939:Epitopes