title: Detection and quantification of cytokines and other biomarkers.
abstract: Accurate measurement of cytokine concentrations is a powerful and essential approach to the study of inflammation. The enzyme-linked immunosorbent assay (ELISA) is a simple, low-cost analytical tool that provides both the specificity and sensitivity required for the study of cytokines in vitro or in vivo. This communication describes a systematic approach to develop an indirect sandwich ELISA to detect and quantify cytokines, or other biomarkers, with accuracy and precision. Also detailed is the use of sequential ELISA assays to analyze multiple cytokines from samples with limited volumes. Finally, the concept of a multiplex ELISA is discussed with considerations given to cost and additional time required for development.
authors.affiliation: Department of Pathology and Laboratory Medicine, Boston University School of Medicine and Boston Medical Center, Boston, MA, USA.
authors.forename: Evan L
authors.initials: EL
authors.lastname: Chiswick
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Duffy
authors.forename: Brian
authors.initials: B
authors.lastname: Japp
authors.forename: Daniel
authors.initials: D
authors.lastname: Remick
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D015415:Biomarkers / Q000032:analysis*; D002021:Buffers; D016207:Cytokines / Q000032:analysis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000191:economics / Q000379:methods*; D006801:Humans; D012680:Sensitivity and Specificity; D012996:Solutions
chemical_list: D000906:Antibodies; D015415:Biomarkers; D002021:Buffers; D016207:Cytokines; D012996:Solutions