title: Haemolytic activity and immunological adjuvant effect of a new steroidal saponin from Allium ampeloprasum var. porrum.
abstract: A new steroidal saponin was isolated from the bulbs of Allium ampeloprasum L. var. porrum. On the basis of chemical evidence, comprehensive spectroscopic analyses, and comparison with known compounds, its structure was established as (3β,5α,6β,25R)-3-{(O-β-D-glucopyranosyl-(1→3)-β-D-glucopyranosyl-(1→2)-O-[O-β-D-glucopyranosyl-(1→3)]-O-β-D-glucopyranosyl-(1→4)-β-D-galactopyranosyl)oxy}-6-hydroxyspirostan-2-one (1). Results of the present study indicated that 1 exhibited haemolytic activity in the in vitro assays, and immunological adjuvant activity on the cellular immune response against ovalbumin antigen.
authors.affiliation: Laboratório de Química de Plantas Medicinais, Núcleo de Pesquisas de Produtos Naturais, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Caixa Postal 68045, CEP 21941-971 Rio de Janeiro, Brazil.
authors.forename: Camila Rodrigues
authors.initials: CR
authors.lastname: Adão
authors.forename: Bernadete
authors.initials: B
authors.lastname: Pereira da Silva
authors.forename: Luzineide Wanderley
authors.initials: LW
authors.lastname: Tinoco
authors.forename: José Paz
authors.initials: JP
authors.lastname: Parente
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D004912:Erythrocytes / Q000187:drug effects*; D006461:Hemolysis; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051379:Mice; D008968:Molecular Conformation; D019697:Onions / Q000737:chemistry*; D010047:Ovalbumin / Q000276:immunology; D012503:Saponins / Q000737:chemistry* / Q000276:immunology / Q000494:pharmacology*; D013150:Spirostans / Q000737:chemistry* / Q000276:immunology / Q000494:pharmacology*; D013256:Steroids / Q000737:chemistry
chemical_list: D000276:Adjuvants, Immunologic; D012503:Saponins; D013150:Spirostans; D013256:Steroids; D010047:Ovalbumin