title: Transcriptional repression by a bZIP protein regulates Dictyostelium prespore differentiation.
abstract: In response to the signaling polyketide DIF-1 DimB directly activates transcription of the ecmB gene in pstB cells; a subset of the prestalk cells that are the precursors of the basal disc. We show that the promoter of pspA, a prespore-specific gene, also contains a DimB binding site. Mutation of this site causes ectopic expression in the prestalk region and ChIP analysis shows that DIF-1 induces binding of DimB to the pspA promoter. DIF-1 represses pspA gene expression in a suspension cell assay but this repression is abrogated in a dimB null strain. These results suggest a coupled control mechanism, whereby the same DIF-DimB signaling pathway that directly activates ecmB gene expression directly represses pspA gene expression.
authors.affiliation: College of Life Sciences, University of Dundee, Dundee, United Kingdom.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Nuñez-Corcuera
authors.forename: Joanna L
authors.initials: JL
authors.lastname: Birch
authors.forename: Yoko
authors.initials: Y
authors.lastname: Yamada
authors.forename: Jeffrey G
authors.initials: JG
authors.lastname: Williams
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D050976:Basic-Leucine Zipper Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001665:Binding Sites; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D002846:Chromatography, Affinity; D004023:Dictyostelium / Q000166:cytology* / Q000187:drug effects / Q000235:genetics*; D024202:Electrophoretic Mobility Shift Assay; D005786:Gene Expression Regulation / Q000187:drug effects; D017930:Genes, Reporter / Q000235:genetics; D006588:Hexanones / Q000494:pharmacology; D006843:Hydrocarbons, Chlorinated / Q000494:pharmacology; D013058:Mass Spectrometry; D008954:Models, Biological; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding / Q000187:drug effects; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D033761:Spores, Protozoan / Q000166:cytology / Q000187:drug effects / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D050976:Basic-Leucine Zipper Transcription Factors; C490570:Differentiation-inducing factor-1, Dictyostelium; D006588:Hexanones; D006843:Hydrocarbons, Chlorinated; D015800:Protozoan Proteins; D012097:Repressor Proteins