title: Effects of vinblastine on microvascular anastomosis healing in diabetic rats.
abstract: OBJECTIVE
  Diabetes mellitus and chemotherapy use are well-known factors that can affect success after microvascular repair. Although many studies have investigated each factor individually, no study exists on their combined effect. The aim of our study was to investigate the effects of preoperative vinblastine, a vinca alkaloid, on the healing of microvascular anastomosis in diabetic and non-diabetic rats.
  
  
  METHODS
  In this study, 144 Sprague Dawley rats were assigned randomly and equally into experimental or control groups. The experimental group consisted of 72 rats which were made diabetic by alloxan injections. Diabetes was confirmed by glycosuria (>2%) and elevated plasma glucose (>300 mg/100 ml) after three days of alloxan injections. The experimental group then received a single dose of intraperitoneal vinblastine. After applying the medication, the two main groups were divided into three subgroups. For each group, we performed end-to-end femoral artery anastomosis on Day 7, 14, and 21, respectively. We further divided each subgroup equally into three smaller groups to test patency and took biopsies at Day 7, 14 and 21, respectively. Histopathologic evaluation was carried out.
  
  
  RESULTS
  The comparison of patency tests and pathologic examination indicated that there was no statistically significant difference between the two groups.
  
  
  CONCLUSIONS
  The negative effects of diabetes and the administration of vinblastine did not significantly influence anastomosis healing.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Şişli Etfal Training and Research Hospital, İstanbul, Turkey. semrakarsidag@yahoo.com
authors.forename: Semra
authors.initials: S
authors.lastname: Karşidağ
authors.forename: Soner
authors.initials: S
authors.lastname: Tatlidede
authors.forename: Uğur
authors.initials: U
authors.lastname: Tosun
authors.forename: Kemalettin
authors.initials: K
authors.lastname: Yildiz
authors.forename: Fevziye
authors.initials: F
authors.lastname: Kabukçuoğlu
authors.forename: Arzu
authors.initials: A
authors.lastname: Akçal
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D001786:Blood Glucose / Q000032:analysis; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000503:physiopathology*; D005263:Femoral Artery / Q000601:surgery*; D008297:Male; D008866:Microsurgery; D051381:Rats; D017207:Rats, Sprague-Dawley; D014654:Vascular Patency; D014747:Vinblastine / Q000494:pharmacology; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D001786:Blood Glucose; D014747:Vinblastine