title: Apelin supports primary rat retinal Müller cells under chemical hypoxia and glucose deprivation.
abstract: Müller cells support the integrity of the blood-retinal barrier, whereas their dysfunction under pathological conditions may contribute to retinal edema formation. The apelin peptide, as the endogenous ligand of G protein-coupled receptor APJ, participates in numbers of physiological and pathological processes. Recent studies highlight its emerging role against ischemic injury. Our study aimed to investigate the potential neuroprotection of apelin for primary rat retinal Müller cells under hypoxia or glucose-deprivation (GD) by cell viability, migration and apoptosis, as well as apelin/APJ immunofluorescence labeling and mRNA expression. The results showed that exogenous apelin significantly stimulated Müller cells viability and migration under normal, hypoxic and glucose-free condition, also prevented apoptosis. Apelin immunoreactivities represented weak and diffuse staining in the cytoplasm, along with restricted nuclear APJ expression. They both appeared stronger immunoreactivities after 12h hypoxia. Under hypoxic stress, apelin mRNA expression began to increase at 6h (9.97 folds, p<0.01), and APJ mRNA also up-regulated (2h 6.50 folds, p<0.05; 4h 2.25 folds, p<0.05; 6h 14 folds, p<0.01), whereas they both down-regulated during 4-12h GD. Our results suggested that apelin induced the tolerance of Müller cells to hypoxia and GD. Its administration might be a promising protection for blood-retinal barrier to ischemia.
authors.affiliation: Department of Ophthalmology, People's Hospital, Peking University, & Key Laboratory of Vision Loss and Restoration, Ministry of Education, Beijing 100044, China.
authors.forename: Xin-Lei
authors.initials: XL
authors.lastname: Wang
authors.forename: Yong
authors.initials: Y
authors.lastname: Tao
authors.forename: Qiang
authors.initials: Q
authors.lastname: Lu
authors.forename: Yan-Rong
authors.initials: YR
authors.lastname: Jiang
mesh_terms: D000818:Animals; D000075244:Apelin Receptors; D017209:Apoptosis; D015687:Cell Hypoxia; D002465:Cell Movement; D002470:Cell Survival; D003035:Cobalt; D019610:Cytoprotection; D005786:Gene Expression Regulation; D005947:Glucose / Q000172:deficiency; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D009457:Neuroglia / Q000502:physiology*; D061251:Primary Cell Culture; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism; D012160:Retina / Q000166:cytology*; D014158:Transcription, Genetic
chemical_list: D000075244:Apelin Receptors; C491284:Aplnr protein, rat; D036341:Intercellular Signaling Peptides and Proteins; D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled; C495199:apelin-13 peptide; D003035:Cobalt; C018021:cobaltous chloride; D005947:Glucose