title: Magnetic field-based delivery of human CD133⁺ cells promotes functional recovery after rat spinal cord injury.
abstract: METHODS
  Experimental animal study of spinal cord injury (SCI), using a cell delivery system.
  
  
  OBJECTIVE
  To investigate the therapeutic effects of transplantation of peripheral blood-derived CD133 cells, with a magnetic delivery system in a rat SCI model.
  
  
  BACKGROUND
  There are no reports on intrathecal transplantation of peripheral blood-derived CD133 cells, with a magnetic cell delivery system to treat SCI.
  
  
  METHODS
  Magnetically isolated peripheral blood-derived CD133 cells were used as the cell source. Contusion SCI was induced by an Infinite Horizon impactor in athymic nude rats. CD133 cells or phosphate-buffered saline was administered via a lumbar puncture immediately after SCI, and a magnetic field was applied to rats for 30 minutes. Animals were analyzed at specific times after transplantation by several methods to examine cell tracking, functional recovery, and histological angiogenesis and neurogenesis.
  
  
  RESULTS
  A combination of cell transplantation and application of a magnetic field at the site of injury caused significant functional recovery. Transplantation of the cells alone in the absence of the magnetic field showed no effect beyond that observed in control rats.
  
  
  CONCLUSIONS
  The combination of intrathecal transplantation of CD133 cells and application of a magnetic field at the site of injury is a possible therapeutic strategy to treat rat SCI and may therefore find application in clinical settings.
authors.affiliation: Department of Orthopaedic Surgery, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan. yuhkif@hotmail.com
authors.forename: Yuki
authors.initials: Y
authors.lastname: Fujioka
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Tanaka
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Nakanishi
authors.forename: Naosuke
authors.initials: N
authors.lastname: Kamei
authors.forename: Toshio
authors.initials: T
authors.lastname: Nakamae
authors.forename: Bunichiro
authors.initials: B
authors.lastname: Izumi
authors.forename: Ryo
authors.initials: R
authors.lastname: Ohta
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Ochi
mesh_terms: D000071916:AC133 Antigen; D042683:Angiopoietin-1 / Q000235:genetics / Q000378:metabolism; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D058948:Cell Tracking / Q000379:methods; D002478:Cells, Cultured; D004195:Disease Models, Animal; D019054:Evoked Potentials, Motor; D005260:Female; D005786:Gene Expression Regulation; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D055909:Magnetic Field Therapy; D008856:Microscopy, Fluorescence; D009043:Motor Activity; D018919:Neovascularization, Physiologic; D055495:Neurogenesis; D010455:Peptides / Q000378:metabolism*; D036102:Peripheral Blood Stem Cell Transplantation / Q000379:methods*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011923:Rats, Nude; D020127:Recovery of Function; D013119:Spinal Cord Injuries / Q000235:genetics / Q000503:physiopathology / Q000628:therapy*; D013129:Spinal Puncture; D013234:Stem Cells / Q000276:immunology* / Q000378:metabolism; D013997:Time Factors; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
chemical_list: D000071916:AC133 Antigen; D042683:Angiopoietin-1; D015703:Antigens, CD; D015415:Biomarkers; D006023:Glycoproteins; D015850:Interleukin-6; C000606023:PROM1 protein, human; D010455:Peptides; C000606025:Prom1 protein, rat; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D042461:Vascular Endothelial Growth Factor A; C467498:vascular endothelial growth factor A, rat; D008156:Luciferases