title: Identification and characterization of an LCAT-like Arabidopsis thaliana gene encoding a novel phospholipase A.
abstract: A previously uncharacterized Arabidopsis lecithin:cholesterol acyltransferase (LCAT) family gene (At4g19860) was functionally expressed in yeast, where it was demonstrated to encode a novel cytosolic and calcium-independent phospholipase A with preferences for the sn-2 position. This enzyme shows optimal activity at pH 5.0, exhibits a headgroup specificity for phosphatidylcholine>phosphatidic acid>phosphatidylethanolamine>phosphatidylglycerol>phosphatidylserine and has an acyl chain specificity for oleoyl>linoleoyl>ricinoleoyl. The expression of AtLCAT-PLA inhibited yeast cell growth and fatty acid accumulation. AtLCAT-PLA transcript in Arabidopsis was detected at high levels in roots and siliques.
authors.affiliation: Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, Canada.
authors.forename: Guanqun
authors.initials: G
authors.lastname: Chen
authors.forename: Michael S
authors.initials: MS
authors.lastname: Greer
authors.forename: Ida
authors.initials: I
authors.lastname: Lager
authors.forename: Jenny Lindberg
authors.initials: JL
authors.lastname: Yilmaz
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Mietkiewska
authors.forename: Anders S
authors.initials: AS
authors.lastname: Carlsson
authors.forename: Sten
authors.initials: S
authors.lastname: Stymne
authors.forename: Randall J
authors.initials: RJ
authors.lastname: Weselake
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D018744:DNA, Plant / Q000235:genetics; D017343:Genes, Plant; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000235:genetics* / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism; D010741:Phospholipases A / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D013379:Substrate Specificity
chemical_list: D029681:Arabidopsis Proteins; D018744:DNA, Plant; D010713:Phosphatidylcholines; D011994:Recombinant Proteins; D007862:Phosphatidylcholine-Sterol O-Acyltransferase; D010741:Phospholipases A