title: Measurement of cholesterol and triglycerides from a dried blood spot in an Indian Council of Medical Research-World Health Organization multicentric survey on risk factors for noncommunicable diseases in India.
abstract: BACKGROUND
  Dried blood may be a convenient method of sample collection in epidemiological studies; however, the method needs evaluation in a field settings. In the present study, feasibility of using dried blood for measurement of cholesterol and triglycerides was evaluated in multicenter surveillance study for noncommunicable disease (NCD).
  
  
  METHODS
  Samples were collected in a cross-sectional study for NCD risk factor surveillance conducted in six centers in India. For every tenth subject recruited, a blood sample was also collected on filter paper. These 10% serum samples and dried blood spots were analyzed for cholesterol and triglycerides.
  
  
  RESULTS
  The mean coefficient of variation (CV) for cholesterol was less than 10% between dried blood and serum in five of the six participating centers. Only one center showed a high CV of 14%. Similarly, the mean bias was less than 10% in five centers. The intraclass correlation between cholesterol values in dried blood and serum were greater than 0.638 in all centers, which suggests a good homogeneity of results. The mean CV for triglycerides ranged from 0.36% to 17.97%. The intraclass correlation between triglyceride values in dried blood and serum ranged from 0.756 to 0.880 in the six centers.
  
  
  CONCLUSIONS
  In conclusion, dried blood would be a good method for collection of blood for measurement of cholesterol and triglycerides for population health surveys. However, the benefits of blood spot analysis should be weighed against potential sources of errors attributable to sampling and other factors, such as temperature and humidity, in a country like India.
authors.affiliation: Department of Cardiac Biochemistry, All India Institute of Medical Sciences, New Delhi-29, India. lakshmy_ram@yahoo.com
authors.forename: Ramakrishnan
authors.initials: R
authors.lastname: Lakshmy
authors.forename: Prashant
authors.initials: P
authors.lastname: Mathur
authors.forename: Ruby
authors.initials: R
authors.lastname: Gupta
authors.forename: Bela
authors.initials: B
authors.lastname: Shah
authors.forename: Krishnan
authors.initials: K
authors.lastname: Anand
authors.forename: Viswanathan
authors.initials: V
authors.lastname: Mohan
authors.forename: Nimesh G
authors.initials: NG
authors.lastname: Desai
authors.forename: Jagdish
authors.initials: J
authors.lastname: Mahanta
authors.forename: Prashant Prabhakar
authors.initials: PP
authors.lastname: Joshi
authors.forename: Kavumpurathu Raman
authors.initials: KR
authors.lastname: Thankappan
mesh_terms: D000293:Adolescent; D000328:Adult; D002784:Cholesterol / Q000097:blood*; D003324:Coronary Artery Disease / Q000097:blood / Q000453:epidemiology; D003430:Cross-Sectional Studies; D059788:Dried Blood Spot Testing / Q000592:standards*; D005240:Feasibility Studies; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012307:Risk Factors; D014280:Triglycerides / Q000097:blood*; D014944:World Health Organization; D055815:Young Adult
chemical_list: D014280:Triglycerides; D002784:Cholesterol