title: β-conglycinin combined with fenofibrate or rosuvastatin have exerted distinct hypocholesterolemic effects in rats.
abstract: BACKGROUND
  There is increasing interest in non-pharmacological control of cholesterol and triglyceride levels in the plasma and diet-drug association represent an important area of studies. The objective of this study was to observe the hypocholesterolemic effect of soybean β-conglycinin (7S protein) alone and combined with fenofibrate and rosuvastatin, two hypolipidemic drugs.
  
  
  METHODS
  The protein and drugs were administered orally once a day to rats and the effects were evaluated after 28 days. Wistar rats were divided into six groups (n = 9): hypercholesterolemic diet (HC), HC+7S protein (300 mg.kg-1 day-1) (HC-7S), HC+fenofibrate (30 mg.kg-1 day-1)(HC-FF), HC+rosuvastatin (10 mg.kg-1 day-1)(HC-RO), HC+7S+fenofibrate (HC-7S-FF) and HC+7S+rosuvastatin (HC-7S-RO).
  
  
  RESULTS
  Animals in HC-7S, HC-FF and HC-RO exhibited reductions of 22.9, 35.8 and 18.8% in total plasma cholesterol, respectively. In HC-7S-FF, animals did not show significant alteration of the level in HC+FF while the group HC-7S-RO showed a negative effect in comparison with groups taking only protein (HC-7S) or drug (HC-RO). The administration of the protein, fenofibrate and rosuvastatin alone caused increases in the plasma HDL-C of the animals, while the protein-drug combinations led to an increase compared to HC-FF and HC-RO. The plasma concentration of triacylgycerides was significantly reduced in the groups without association, while HC-7S-FF showed no alteration and HC-7S-RO a little reduction.
  
  
  CONCLUSIONS
  The results of our study indicate that conglycinin has effects comparable to fenofibrate and rosuvastatin on the control of plasma cholesterol, HDL-C and triacylglycerides, when given to hypercholesterolemic rats, and suggests that the association of this protein with rosuvastatin alters the action of drug in the homeostasis of cholesterol.
authors.affiliation: Department of Food and Nutrition, School of Pharmaceutical Sciences, São Paulo State University -UNESP, Araraquara, SP, Brazil.
authors.forename: Ederlan S
authors.initials: ES
authors.lastname: Ferreira
authors.forename: Maraiza A
authors.initials: MA
authors.lastname: Silva
authors.forename: Aureluce
authors.initials: A
authors.lastname: Demonte
authors.forename: Valdir A
authors.initials: VA
authors.lastname: Neves
mesh_terms: D000818:Animals; D000924:Anticholesteremic Agents / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D052179:Antigens, Plant / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D002784:Cholesterol / Q000097:blood / Q000378:metabolism; D059305:Diet, High-Fat / Q000009:adverse effects; D004044:Dietary Proteins / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D004338:Drug Combinations; D004357:Drug Synergism; D011345:Fenofibrate / Q000494:pharmacology* / Q000627:therapeutic use; D005464:Fluorobenzenes / Q000494:pharmacology* / Q000627:therapeutic use; D005916:Globulins / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D006321:Heart / Q000187:drug effects; D006937:Hypercholesterolemia / Q000097:blood / Q000188:drug therapy / Q000209:etiology; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D009206:Myocardium / Q000473:pathology; D009929:Organ Size / Q000187:drug effects; D011743:Pyrimidines / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D000068718:Rosuvastatin Calcium; D055314:Seed Storage Proteins / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D030262:Soybean Proteins / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D013025:Glycine max; D013449:Sulfonamides / Q000494:pharmacology* / Q000627:therapeutic use; D014280:Triglycerides / Q000097:blood / Q000378:metabolism
chemical_list: D000924:Anticholesteremic Agents; D052179:Antigens, Plant; D004044:Dietary Proteins; D004338:Drug Combinations; D005464:Fluorobenzenes; D005916:Globulins; D011743:Pyrimidines; D055314:Seed Storage Proteins; D030262:Soybean Proteins; D013449:Sulfonamides; D014280:Triglycerides; C013894:beta-conglycinin protein, Glycine max; D000068718:Rosuvastatin Calcium; D002784:Cholesterol; D011345:Fenofibrate