title: Single-layer CVD-grown graphene decorated with metal nanoparticles as a promising biosensing platform.
abstract: A new approach to the development of a single-layer graphene sensor decorated with metal nanoparticles is presented. Chemical vapor deposition is used to grow single layer graphene on copper. Decoration of the single-layer graphene is achieved by electroless deposition of Au nanoparticles using the copper substrate as a source of electrons. Transfer of the decorated single-layer graphene on glassy carbon electrodes offers a sensitive platform for biosensor development. As a proof of concept, 10 units of glucose oxidase were deposited on the surface in a Nafion matrix to stabilize the enzyme as well as to prevent interference from ascorbic acid and uric acid. Amperometric linear response calibration in the μmoll(-1) is obtained. The presented methodology enables highly sensitive platforms for biosensor development, providing a scalable roll-to-roll production with a much more reproducible scheme when compared to the graphene biosensors reported previously based on drop-cast of multi-layer graphene suspensions.
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of California, Berkeley, CA 94720, United States. agutes@gmail.com
authors.forename: Albert
authors.initials: A
authors.lastname: Gutés
authors.forename: Carlo
authors.initials: C
authors.lastname: Carraro
authors.forename: Roya
authors.initials: R
authors.lastname: Maboudian
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*
chemical_list: D006108:Graphite; D005949:Glucose Oxidase; D005947:Glucose