title: In situ monitoring of the DNA hybridization by attenuated total reflection surface-enhanced infrared absorption spectroscopy.
abstract: In situ monitoring of DNA hybridization kinetics is achieved via an attenuated total reflection surface-enhanced infrared absorption spectroscopy (ATR-SEIRAS) technique using a sandwich assay structure. The synergistic enhancement effect gives this ATR-SEIRAS-based detection strategy promise to be a convenient and unique platform for bioanalysis.
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.
authors.forename: Jian-Yun
authors.initials: JY
authors.lastname: Xu
authors.forename: Bo
authors.initials: B
authors.lastname: Jin
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhao
authors.forename: Kang
authors.initials: K
authors.lastname: Wang
authors.forename: Xing-Hua
authors.initials: XH
authors.lastname: Xia
mesh_terms: D004247:DNA / Q000378:metabolism*; D017403:In Situ Hybridization / Q000295:instrumentation / Q000379:methods*; D007700:Kinetics; D008722:Methods; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D013499:Surface Properties
chemical_list: D004247:DNA