title: Mutational biosynthesis of ansamitocin antibiotics: a diversity-oriented approach to exploit biosynthetic flexibility.
abstract: New ansamitocin derivatives were prepared by feeding aminobenzoic acid derivatives to cultures of Actinosynnema pretiosum HGF073, a mutant strain blocked in the biosynthesis of the required 3-amino-5-hydroxybenzoic acid (AHBA) starter unit. Use of several aminobenzoic acids as precursors led to a spectrum of products, reflecting the sequence of post-PKS tailoring steps involved in the generation of ansamitocins and adding novel aspects to the published suggestion model of post-PKS tailoring logic and flexibility. The studies provide insights into the substrate flexibility of the enzymes required for ansamitocin biosynthesis in A. pretiosum, whereas preliminary biological testing of the derivatives isolated and fully characterized by NMR spectroscopy allowed structure-activity relationship assignments to be made for a variety of intermediates occurring during the post-PKS tailoring sequence in ansamitocin biosynthesis.
authors.affiliation: Zentrum für Biomolekulare Wirkstoffe (BMWZ), Leibniz Universität, Hannover Schneiderberg 1B, 30167 Hannover, Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Knobloch
authors.forename: Kirsten
authors.initials: K
authors.lastname: Harmrolfs
authors.forename: Florian
authors.initials: F
authors.lastname: Taft
authors.forename: Binia
authors.initials: B
authors.lastname: Thomaszewski
authors.forename: Florenz
authors.initials: F
authors.lastname: Sasse
authors.forename: Andreas
authors.initials: A
authors.lastname: Kirschning
mesh_terms: D000192:Actinomycetales / Q000201:enzymology / Q000235:genetics; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry; D008453:Maytansine / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000737:chemistry; D015394:Molecular Structure; D016296:Mutagenesis; D013329:Structure-Activity Relationship
chemical_list: D000900:Anti-Bacterial Agents; D008453:Maytansine; C015682:ansamitocins