title: Isolation and differentiation of monocytes-macrophages from human blood.
abstract: The prevalence of fungal infections remains high, and it is associated with significant mortality and morbidity. Macrophages are heterogeneous population of effectors enriched in regions of Candida colonization. These cells sense Candida, and are critical in the resolution of these infections. Here, we describe how macrophages are generated in the presence of colony-stimulating factor-1 (CSF-1); an important cytokine required for the survival, proliferation and ex-vivo differentiation of monocytes to macrophages.
authors.affiliation: Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia, QLD, Australia.
authors.forename: Dipti
authors.initials: D
authors.lastname: Vijayan
mesh_terms: D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient / Q000379:methods; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000378:metabolism; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D008264:Macrophages / Q000166:cytology*; D009000:Monocytes / Q000166:cytology* / Q000378:metabolism
chemical_list: D018950:Lipopolysaccharide Receptors