title: STIM1 is required for attenuation of PMCA-mediated Ca2+ clearance during T-cell activation.
abstract: T-cell activation involves a complex signalling cascade uniquely dependent on elevated cytosolic Ca(2+) levels. Further, the spatiotemporal characteristics of this Ca(2+) signal play a critical role in this process via selective activation of transcription factors. In T cells, store-operated Ca(2+) entry (SOCe) is the primary Ca(2+) influx pathway; however, cytosolic Ca(2+) concentration depends upon the balance between Ca(2+) influx and extrusion. The plasma membrane Ca(2+) ATPase (PMCA) has previously been identified as a critical player in Ca(2+) clearance in T cells. Here, we provide data revealing both functional and physical links between the activation of stromal interacting molecule 1 (STIM1) and PMCA-mediated Ca(2+) clearance. Due to the ubiquitous expression of both STIM1 and PMCA, these findings have wide-ranging implications for Ca(2+) signalling in multiple cell types.
authors.affiliation: Department of Biochemistry, Temple University School of Medicine, Philadelphia, PA 19140, USA.
authors.forename: Michael F
authors.initials: MF
authors.lastname: Ritchie
authors.forename: Elsie
authors.initials: E
authors.lastname: Samakai
authors.forename: Jonathan
authors.initials: J
authors.lastname: Soboloff
mesh_terms: D002118:Calcium / Q000378:metabolism*; D002413:Cations, Divalent / Q000378:metabolism; D002460:Cell Line; D003600:Cytosol / Q000737:chemistry; D006801:Humans; D008213:Lymphocyte Activation; D008565:Membrane Proteins / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000378:metabolism*; D053499:Plasma Membrane Calcium-Transporting ATPases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D015398:Signal Transduction; D000071737:Stromal Interaction Molecule 1; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
chemical_list: D002413:Cations, Divalent; D008565:Membrane Proteins; D009363:Neoplasm Proteins; C103530:STIM1 protein, human; D000071737:Stromal Interaction Molecule 1; D053499:Plasma Membrane Calcium-Transporting ATPases; D002118:Calcium