title: An improved method for isolation of RNA from bone.
abstract: BACKGROUND
  Bone physiology is increasingly appreciated as an important contributor to metabolic disorders such as type 2 diabetes. However, progress in understanding the role of bone in determining metabolic health is hampered by the well-described difficulty of obtaining high quality RNA from bone for gene expression analysis using the currently available approaches.
  
  
  RESULTS
  We developed a simple approach to isolate bone RNA that combines pulverizing the bone and the phenol-guanidinium based RNA extraction in a single step while maintaining near-freezing temperatures. This single step method increases the yield of high quality RNA by eight-fold, with RNA integrity numbers ranging from 6.7 to 9.2.
  
  
  CONCLUSIONS
  Our streamlined approach substantially increases the yield of high-quality RNA from bone tissue while facilitating safe and efficient processing of multiple samples using readily available platforms. The RNA obtained from this method is suitable for use in gene expression analysis in real-time quantitative PCR, microarray, and next generation sequencing applications.
authors.affiliation: Ubiquitin Biology Laboratory, Pennington Biomedical Research Center, 6400 Perkins Road, Baton Rouge, LA 70808, USA.
authors.forename: Lauren E
authors.initials: LE
authors.lastname: Carter
authors.forename: Gail
authors.initials: G
authors.lastname: Kilroy
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Gimble
authors.forename: Z Elizabeth
authors.initials: ZE
authors.lastname: Floyd
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000737:chemistry* / Q000378:metabolism*; D003080:Cold Temperature; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics; D019791:Guanidine / Q000737:chemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010636:Phenols / Q000737:chemistry; D012313:RNA / Q000302:isolation & purification*
chemical_list: D010636:Phenols; D012313:RNA; D019791:Guanidine