title: Anthocyanin composition of wild Colombian fruits and antioxidant capacity measurement by electron paramagnetic resonance spectroscopy.
abstract: The qualitative and quantitative anthocyanin composition of four wild tropical fruits from Colombia was studied. Compounds of "mora pequeña" ( Rubus megalococcus  Focke.), "uva de árbol" ( Myrciaria aff. cauliflora  O. Berg), coral, and motilón ( Hyeronima macrocarpa  Mull. Arg.) fruits were separately extracted with methanol-acetic acid (95:5, v/v). The anthocyanin-rich extracts (AREs) were obtained by selective adsorption on Amberlite XAD-7. Each extract was analyzed by HPLC-PDA and HPLC-HRESI-MS(n) with LCMS-IT-TOF equipment in order to characterize the anthocyanin pigments and the coinjection in HPLC using standards allowed identifying the major constituents in each extract. The antioxidant activity was measured by electron paramagnetic resonance (EPR) and UV-vis spectroscopy, using ABTS and DPPH free radicals. The ARE of motilón ( H. macrocarpa  Müll. Arg) exhibited the highest radical scavenging activity in comparison to the other extracts. A second-order kinetic model was followed in all of the cases. These results suggested that the studied fruits are promising not only as source of natural pigments but also as antioxidant materials for food industry.
authors.affiliation: Departamento de Química, Universidad Nacional de Colombia, AA 14490, Bogotá, Colombia.
authors.forename: Liliana
authors.initials: L
authors.lastname: Santacruz
authors.forename: José G
authors.initials: JG
authors.lastname: Carriazo
authors.forename: Ovidio
authors.initials: O
authors.lastname: Almanza
authors.forename: Coralia
authors.initials: C
authors.lastname: Osorio
mesh_terms: D000872:Anthocyanins / Q000032:analysis*; D000975:Antioxidants / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D003105:Colombia; D004578:Electron Spin Resonance Spectroscopy; D019665:Euphorbiaceae / Q000737:chemistry; D005638:Fruit / Q000737:chemistry*; D027822:Myrtaceae / Q000737:chemistry; D027824:Rosaceae / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000872:Anthocyanins; D000975:Antioxidants