title: Studies on serum 8-hydroxy guanosine (8-OHdG) as reliable biomarker for psoriasis.
abstract: BACKGROUND
  Oxidative stress was implicated in the psoriasis disease development and may damage DNA leading to keratinocytes cell death. No serum biomarker was available for the oxidative DNA damage.
  
  
  OBJECTIVE
  To evaluate the 8-OHdG (8-Hydroxy guanosine) as reliable biomarker for the oxidative stress in psoriatic patients with severity.
  
  
  METHODS
  A total of 30 patients were considered for the study and graded according to the Psoriasis Area Severity Index (PASI) and 10 healthy controls. Blood was collected under aseptic condition, and serum was separated. Serum 8-OHdG and total antioxidant capacity was measured by competitive enzyme linked immunosorbent assay using `8-OHdG Check' and PAO kit (JaICA, Fukuroi City, Japan).
  
  
  RESULTS
  The average serum 8-OHdG level in the control, mild, moderate and severe groups were 1.18 ± 0.93 ng/mL, 3.46 ± 0.82 ng/mL, 3.68 ± 0.67 ng/mL and 4.86 ± 1.7 ng/mL respectively. There was no significant difference in the average level of total antioxidant capacity of control, mild, moderate and severe groups, and the values presented were 295.88 ± 206 μmol/L, 1392.20 ± 225 μmol/L, 1199.57 ± 257 μmol/L and 1184.24 ± 207 μmol/L respectively.
  
  
  CONCLUSIONS
  Serum 8-OHdG levels could be used as good biomarker for the early diagnosis of psoriasis and its management.
authors.affiliation: Department of Dermatology, JSS Medical College, JSS University, Mysore, India. basavarajkh@yahoo.co.in
authors.forename: K H
authors.initials: KH
authors.lastname: Basavaraj
authors.forename: P
authors.initials: P
authors.lastname: Vasu Devaraju
authors.forename: K S
authors.initials: KS
authors.lastname: Rao
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood*; D006151:Guanosine / Q000031:analogs & derivatives* / Q000097:blood; D006801:Humans; D008875:Middle Aged; D011565:Psoriasis / Q000097:blood / Q000175:diagnosis*
chemical_list: D015415:Biomarkers; D006151:Guanosine; C046215:8-hydroxyguanosine