title: Mn-doped ZnSe d-dots-based α-methylacyl-CoA racemase probe for human prostate cancer cell imaging.
abstract: In this paper, we report the successful use of non-cadmium-based Mn-doped ZnSe d-dots (Mn/ZnSe) as highly efficient and nontoxic optical probes for human prostate cancer cells imaging. Mn/ZnSe d-dots are directly prepared in aqueous solution. The α-methylacyl-CoA racemase (AMACR) is overexpressed in prostate cancers; the presence of antibodies specific for AMACR is more sensitive and specific than serum prostate specific antigen levels in distinguishing patients with prostate cancers. Mn/ZnSe d-dots were linked to anti-AMACR to form Mn/ZnSe d-dots-anti-AMACR bioconjugates for the direct prostate cancer cell imaging. 3-(4,5-Dimethylthiazol-2-yl)-2 and 5-diphenyl tetrazolium bromide assay demonstrated that Mn/ZnSe d-dots exhibited favorable cytocompatibility to LNCaP cells with high concentration (1 mM) and long-time incubation (24 h). Furthermore, cellular imaging results demonstrated that Mn/ZnSe d-dots were remarkably efficacious for high-specificity cell imaging. The antibody-mediated delivery of the bioconjugates was further confirmed by the observation of no fluorescence signals in vitro targeting in nonprostate-cancer-based cell lines which are negative for AMACR. Mn/ZnSe d-dots as non-cadmium-based safe and efficient optical imaging nanoprobes could therefore be used for targeting imaging and treatment of cancers in the early stage.
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University, Qianwei Road 10, Changchun 130012, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Gao
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Xingguang
authors.initials: X
authors.lastname: Su
mesh_terms: D000728:Androgens / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000032:analysis*; D002104:Cadmium / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival; D006801:Humans; D008297:Male; D008345:Manganese; D011471:Prostatic Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D045663:Quantum Dots; D019998:Racemases and Epimerases / Q000032:analysis*; D018036:Selenium Compounds / Q000737:chemistry; D012680:Sensitivity and Specificity; D013778:Tetrazolium Salts / Q000737:chemistry; D013844:Thiazoles / Q000737:chemistry; D017967:Zinc Compounds / Q000737:chemistry
chemical_list: D000728:Androgens; D014408:Biomarkers, Tumor; D018036:Selenium Compounds; D013778:Tetrazolium Salts; D013844:Thiazoles; D017967:Zinc Compounds; D002104:Cadmium; D008345:Manganese; D019998:Racemases and Epimerases; C108352:alpha-methylacyl-CoA racemase; C022616:thiazolyl blue; C044696:zinc selenide