title: [Pollen germination in vitro and pollen tube growth of Scutellaria baicalensis].
abstract: OBJECTIVE
  To study the pollen germination in vitro and pollen tube growth of Scutellaria baicalensis.
  
  
  METHODS
  Two years-old S. baicalensis that in the experiment field of Shangluo university were used as the materials. The effects of culture medium, sucrose, PEG, pH value, culture time and temperature on the pollen germination and tube growth of S. baicalensis were investigated using the method of liquid culture.
  
  
  CONCLUSIONS
  The results showed that 100 g x L(-1) sucrose was helpful for the germination and growth of pollens. However, higher concentration of sucrose would inhibit strongly pollen germination and the growth of pollen tubes. PEG could accelerate pollen germination and tube growth, and the optimal concentration was 150 g x L(-1). The optimum culture medium was BK and BK + 10% sucrose + 150 g x L(-1) PEG (pH 5.8) , and the latter was more benefit for pollen germination and tube growth. The pollen of germination was better at 25-35 degrees C, and it was the best at 30 degrees C for 2-3 hours, while pollen tube growth was slower at 25 degrees C.
authors.affiliation: Biological Medicine Engineering Department, Shangluo University, Shangluo 726000, China. lzgworking@163.com
authors.forename: Zigang
authors.initials: Z
authors.lastname: Liu
authors.forename: Tianming
authors.initials: T
authors.lastname: Hu
authors.forename: Yali
authors.initials: Y
authors.lastname: Yang
mesh_terms: D018929:Cell Culture Techniques; D002478:Cells, Cultured; D003470:Culture Media / Q000378:metabolism; D018525:Germination; D006863:Hydrogen-Ion Concentration; D053205:Pollen Tube / Q000254:growth & development* / Q000378:metabolism; D031364:Scutellaria baicalensis / Q000254:growth & development* / Q000378:metabolism; D013696:Temperature
chemical_list: D003470:Culture Media