title: [Determination of longistylin A and longistylin C in Cajanus cajan].
abstract: OBJECTIVE
  To establish quality control criteria for medicinal herb Cajanus cajan based on the determination of longistylin A and longistylin C, two bioactive and specific stilbenes of the plant.
  
  
  METHODS
  Longistylin A and longistylin C were obtained from the leaves of C. cajan by silica gel column chromatography and identified as marker compounds of this plant by spectroscopic analysis. A RP-HPLC method was established to determine the two compounds.
  
  
  RESULTS
  Longistylin A and longistylin C were well separated on a Thermo BDS Hypersil C18 column (4.6 mm x 250 mm, 5 microm) with a mobile phase methanol-water (8:2), and showed good linearity in the range of 0.00288 - 0.0576 microg and 0.0112 - 0.224 microg, respectively. The average recoveries were 98.9% and 97.2% with RSD of 2.4% and 2.2% for these two compounds, respectively.
  
  
  CONCLUSIONS
  The established analysis method is simple and accurate, whicn can be used for quality control of C. cajan.
authors.affiliation: Tropical Medicine Institute, Guangzhou University of Chinese Medicine, Guangzhou 510405, China. dwwang66@163.com
authors.forename: Dawu
authors.initials: D
authors.lastname: Wang
authors.forename: Minxun
authors.initials: M
authors.lastname: Xiao
authors.forename: Yirong
authors.initials: Y
authors.lastname: Li
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Shen
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Lu
authors.forename: Kanglun
authors.initials: K
authors.lastname: Liu
authors.forename: Zhenghong
authors.initials: Z
authors.lastname: Li
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Hu
mesh_terms: D036561:Cajanus / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004054:Diethylstilbestrol / Q000032:analysis* / Q000302:isolation & purification; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry
chemical_list: D004365:Drugs, Chinese Herbal; D004054:Diethylstilbestrol