title: Coordinate-bond-dependent solid-phase organic synthesis of biotinylated desferrioxamine B: a new route for metal-specific probes.
abstract: Desferrioxamine B (DFOB) was biotinylated at the pendant amine using solid-phase organic synthesis (SPOS) on a matrix used conventionally for metal affinity chromatography. The strength of the DFOB-matrix coordinate bonds was functionally equivalent to a covalent bond which underpinned the veracity of the SPOS format. After washing excess reagents, biotin-DFOB was eluted from the matrix with water at pH 6.
authors.affiliation: School of Medical Sciences (Pharmacology) and Bosch Institute, University of Sydney, New South Wales 2006, Australia.
authors.forename: Tulip
authors.initials: T
authors.lastname: Lifa
authors.forename: Najwa
authors.initials: N
authors.lastname: Ejje
authors.forename: Rachel
authors.initials: R
authors.lastname: Codd
mesh_terms: D001710:Biotin / Q000737:chemistry; D019921:Biotinylation; D002846:Chromatography, Affinity; D003676:Deferoxamine / Q000138:chemical synthesis / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008670:Metals / Q000737:chemistry*; D060327:Solid-Phase Synthesis Techniques; D014867:Water / Q000737:chemistry
chemical_list: D008670:Metals; D014867:Water; D001710:Biotin; D003676:Deferoxamine