title: Influence of incubation conditions on hydrolysis efficiency and iodine enrichment in baker's yeast.
abstract: The influence of incubation conditions, enzyme type, hydrolysis time, and potassium iodide concentration on hydrolysis and iodine enrichment were studied in supernatant and pellets of Saccharomyces cervisiae hydrolysates. The type of enzyme used and incubation time significantly influence hydrolysis efficiency and protein concentration in supernatant and pellet. The highest protein hydrolysis efficiency was obtained by 24-h incubation with papain. Significantly lower values were observed for pepsin and autolysis. The potassium iodide concentration influences the iodine content of supernatant and pellet, but not hydrolysis. Iodide enrichment of supernatant and pellet depends on the concentration of iodide using during incubation. High concentration of iodide and long incubation times were the conditions for optimal iodide enrichment and high-protein hydrolysates. The optimal hydrolysis efficiency and iodine enrichment were obtained during 24-h incubation with papain in a 4.5-mM potassium iodide medium. The efficiency reached 98.22% with iodine concentrations of 2,664.91 and 9,200.67 μg/g iodine in pellet and supernatant, respectively.
authors.affiliation: Department of Applied Pharmacy and Drug Technology, Medical University of Silesia, Sosnowiec, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Dolińska
authors.forename: Michał
authors.initials: M
authors.lastname: Zieliński
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Dobrzański
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Chojnacka
authors.forename: Sebastian
authors.initials: S
authors.lastname: Opaliński
authors.forename: Florian
authors.initials: F
authors.lastname: Ryszka
mesh_terms: D003470:Culture Media / Q000494:pharmacology; D019649:Food Industry / Q000379:methods; D006868:Hydrolysis; D007218:Industrial Microbiology / Q000379:methods; D007455:Iodine / Q000378:metabolism*; D007700:Kinetics; D010206:Papain / Q000378:metabolism; D010434:Pepsin A / Q000378:metabolism; D011193:Potassium Iodide / Q000494:pharmacology; D059748:Proteolysis; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D013997:Time Factors
chemical_list: D003470:Culture Media; D029701:Saccharomyces cerevisiae Proteins; D011193:Potassium Iodide; D007455:Iodine; D010206:Papain; D010434:Pepsin A