title: Study of genetic stability of human bone marrow multipotent mesenchymal stromal cells.
abstract: Immunophenotype, proliferation rate, and genetic stability parameters of bone marrow multipotent mesenchymal stromal cells were studied. Despite the reduction of proliferative activity by passages 11-12, the cells retained the characteristic immunophenotype. The incidence of spontaneous aneuploidy for autosomes 6, 8, 11 and sex chromosomes was evaluated. Two cultures of mesenchymal stromal cells carrying aneuploid cell clones were detected: with chromosome 8 trisomy and X chromosome monosomy. The results indicate the possibility of genetic transformation and selection of mesenchymal stromal cells with abnormal karyotype during in vitro culturing.
authors.affiliation: Medical Genetic Research Center,Russian Academy of Medical Sciences, Moscow, Russia. vanikitina@mail.ru
authors.forename: V A
authors.initials: VA
authors.lastname: Nikitina
authors.forename: E Yu
authors.initials: EY
authors.lastname: Osipova
authors.forename: L D
authors.initials: LD
authors.lastname: Katosova
authors.forename: S A
authors.initials: SA
authors.lastname: Rumyantsev
authors.forename: E V
authors.initials: EV
authors.lastname: Skorobogatova
authors.forename: T V
authors.initials: TV
authors.lastname: Shamanskaya
authors.forename: N P
authors.initials: NP
authors.lastname: Bochkov
mesh_terms: D000782:Aneuploidy; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D005260:Female; D014644:Genetic Variation; D006801:Humans; D016130:Immunophenotyping; D007621:Karyotyping; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D039902:Multipotent Stem Cells / Q000166:cytology*; D010641:Phenotype