title: Arrhenius plot for a reaction catalyzed by a single molecule of β-galactosidase.
abstract: The activity of a single enzyme molecule of Escherichia coli β-galactosidase was measured using a capillary electrophoresis continuous flow assay. As the enzyme molecule traversed the capillary the incubation temperature was increased from 27 to 37 °C, providing a continuous record of the change in rate with temperature. This data was used to develop a single enzyme molecule Arrhenius plot, from which the activation energy of the reaction was determined to be 31 kJ mol(-1).
authors.affiliation: Chemistry Department, University of Winnipeg, 515 Portage Avenue, Winnipeg, Manitoba, Canada R3B 2E9. d.craig@uwinnipeg.ca
authors.forename: Douglas B
authors.initials: DB
authors.lastname: Craig
authors.forename: Linden N
authors.initials: LN
authors.lastname: Chase
mesh_terms: D002384:Catalysis; D019075:Electrophoresis, Capillary; D004926:Escherichia coli / Q000201:enzymology*; D006358:Hot Temperature; D007700:Kinetics; D013816:Thermodynamics; D001616:beta-Galactosidase / Q000378:metabolism*
chemical_list: D001616:beta-Galactosidase