title: Role of the purinergic system in patients with cervical intraepithelial neoplasia and uterine cancer.
abstract: BACKGROUND
  Cervical cancer remains the second leading cause of death among women. Intraepithelial neoplasias and uterine invasive cancer are frequently associated with disturbances in coagulation and changes in the concentrations of adenine nucleotides. This work intended to analyze changes in extracellular adenine nucleotide hydrolysis and blood platelet aggregation in patients diagnosed for cervical intraepithelial neoplasia in different stages as well as uterine invasive cancer.
  
  
  METHODS
  NTPDase, E-NPP, 5'-nucleotidase, total ADA and its isoforms (ADA1 and ADA2), as well as the platelet aggregation from patients with different stages of cervical intraepithelial neoplasia (NICs I, NIC II, NIC III) and uterine invasive cancer were verified.
  
  
  RESULTS
  Neither ATP hydrolysis nor E-NPP activity was changed by the neoplasia stage. On the other hand, ADP and AMP hydrolysis as well as ADA activity were enhanced in NIC I group. AMP hydrolysis was also increased in the cancer group. ADA 1 was the ADA isoform found in platelets from both control and patient groups.
  
  
  CONCLUSIONS
  Our results showed for the first time that NTPDase, 5'-nucleotidase, E-NPP and ADA are not sensible regarding the grade of neoplasia development, since no significant difference was found between the groups studied. Only ADP hydrolysis and ADA activity showed a significant enhancement in NIC I group related to the other stages possibly as a result of the beginning of the neoplasic transformation. This increase could be reflecting a body's reaction against the probable high adenosine levels. We propose for the first time that the ADA isoform present in platelets is ADA 1.
authors.affiliation: Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Avenida Roraima, 97105-900 Santa Maria, RS, Brazil. paulam2208@yahoo.com.br
authors.forename: Paula Acosta
authors.initials: PA
authors.lastname: Maldonado
authors.forename: Victor Camera
authors.initials: VC
authors.lastname: Pimentel
authors.forename: Luiz Augusto
authors.initials: LA
authors.lastname: Negrini
authors.forename: Vera Maria
authors.initials: VM
authors.lastname: Morsch
authors.forename: Maria Rosa Chitolina
authors.initials: MR
authors.lastname: Schetinger
mesh_terms: D000243:Adenosine Deaminase / Q000378:metabolism*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D006868:Hydrolysis; D007527:Isoenzymes; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D010974:Platelet Aggregation; D002583:Uterine Cervical Neoplasms / Q000201:enzymology / Q000473:pathology*; D002578:Uterine Cervical Dysplasia / Q000201:enzymology / Q000473:pathology*
chemical_list: D007527:Isoenzymes; D000243:Adenosine Deaminase