title: Evaluating descriptors for the lateral translocation of membrane proteins.
abstract: Microscopic images of tissue sections are used for diagnosis and monitoring of therapy, by analysis of protein patterns correlating to disease states. Spatial protein distribution is influenced by protein translocation between different membrane compartments and quantified by comparison of microscopic images of biological samples. Cholestatic liver diseases are characterized by translocation of transport proteins, and quantification of their dislocation offers new diagnostic options. However, reliable and unbiased tools are lacking. The nowadays used manual method is slow, subjective and error-prone. We have developed a new workflow based on automated image analysis and improved it by the introduction of scale-free descriptors for the translocation quantification. This fast and unbiased method can substitute the manual analysis, and the suggested descriptors perform better than the earlier used statistical variance.
authors.affiliation: Fraunhofer Institute for Applied Information Technology FIT, Schloss Birlinghoven, 53754 Sankt, Augustin, Germany.
authors.forename: Olga
authors.initials: O
authors.lastname: Domanova
authors.forename: Stefan
authors.initials: S
authors.lastname: Borbe
authors.forename: Stefanie
authors.initials: S
authors.lastname: Mühlfeld
authors.forename: Martin
authors.initials: M
authors.lastname: Becker
authors.forename: Ralf
authors.initials: R
authors.lastname: Kubitz
authors.forename: Dieter
authors.initials: D
authors.lastname: Häussinger
authors.forename: Thomas
authors.initials: T
authors.lastname: Berlage
mesh_terms: D000818:Animals; D001331:Automation; D001647:Bile Acids and Salts / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism*; D002779:Cholestasis / Q000473:pathology*; D019295:Computational Biology / Q000379:methods; D015195:Drug Design; D001330:Electronic Data Processing; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008099:Liver / Q000378:metabolism / Q000473:pathology*; D008565:Membrane Proteins / Q000378:metabolism*; D008871:Microvilli / Q000378:metabolism; D008954:Models, Biological; D008962:Models, Theoretical; D021381:Protein Transport; D051381:Rats; D012815:Signal Processing, Computer-Assisted
chemical_list: D001647:Bile Acids and Salts; D008565:Membrane Proteins