title: Mucin depleted foci, colonic preneoplastic lesions lacking Muc2, show up-regulation of Tlr2 but not bacterial infiltration.
abstract: Mucin depleted foci (MDF) are precancerous lesions of the colon in carcinogen-treated rodents and humans at high risk. Since MDF show signs of inflammation we hypothesized that the defective mucous production would expose them to the risk of being penetrated by intestinal bacteria, which can be sensed by Toll-like receptors (Tlrs) and activate inflammatory pathways. To verify this hypothesis we tested the expression of 84 genes coding for Tlrs and associated pathways using RT-qPCR in MDF (n = 7) from 1,2-dimethylhydrazine (DMH)-treated rats. Among the 84 tested genes, 26 were differentially expressed in MDF with 5 genes significantly up-regulated and 21 down-regulated when compared to the normal mucosa. Tlr2, as well as other downstream genes (Map4k4, Hspd1, Irak1, Ube2n), was significantly up-regulated. Among the genes regulating the NFkB pathway, only Map4k4 was significantly up-regulated, while 19 genes were not varied and 6 were down-regulated. Tlr2 protein was weakly expressed both in normal mucosa and MDF. To determine whether inflammation observed in MDF could be caused by bacteria contacting or infiltrating crypts, we performed fluorescence in situ hybridization (FISH) experiments with a rRNA universal bacterial probe. None of the 21 MDF tested, showed bacteria inside the crypts, while among the colonic tumors (n = 15), only one had very few bacteria on the surface and on the surrounding normal mucosa. In conclusion, the up-regulation of Tlr2 in MDF, suggests a link between this receptor and carcinogenesis, possibly related to a defective barrier function of these lesions. The data of FISH experiments do not support the hypothesis that inflammation in MDF and tumors is stimulated by bacterial infiltration.
authors.affiliation: Department of Pharmacology, University of Florence, Florence, Italy.
authors.forename: Angelo Pietro
authors.initials: AP
authors.lastname: Femia
authors.forename: Alexander
authors.initials: A
authors.lastname: Swidsinski
authors.forename: Piero
authors.initials: P
authors.lastname: Dolara
authors.forename: Maddalena
authors.initials: M
authors.lastname: Salvadori
authors.forename: Amedeo
authors.initials: A
authors.lastname: Amedei
authors.forename: Giovanna
authors.initials: G
authors.lastname: Caderni
mesh_terms: D000818:Animals; D001419:Bacteria / Q000378:metabolism*; D003106:Colon / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D003110:Colonic Neoplasms / Q000235:genetics* / Q000382:microbiology*; D015536:Down-Regulation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D055262:Mucin-2 / Q000172:deficiency* / Q000378:metabolism; D011230:Precancerous Conditions / Q000382:microbiology / Q000473:pathology*; D051381:Rats; D011916:Rats, Inbred F344; D015398:Signal Transduction / Q000235:genetics; D051195:Toll-Like Receptor 2 / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation / Q000235:genetics*
chemical_list: D055262:Mucin-2; C495343:Tlr2 protein, rat; D051195:Toll-Like Receptor 2