title: Lactic acid bacteria from raw milk as potentially beneficial strains to prevent bovine mastitis.
abstract: Bovine mastitis produces a wide variety of problems in the dairy farm. The treatment of this disease is based on the use of antibiotics which are not always effective. These drugs are also responsible for the presence of residues in the milk and the increase of antibiotic-resistant strains. Probiotic products were proposed as a valid alternative to antibiotic therapies and are also useful for the prevention of infectious syndromes. With the aim of designing a probiotic product to prevent bovine mastitis, lactic acid bacteria (LAB) were isolated from foremilk samples from different dairy farms in Córdoba-Argentina. One hundred and seventeen LAB were isolated and their beneficial characteristics such as the production of inhibitory substances, surface properties and production of exopolysaccharides (EPS) were assessed. Most of them displayed low degree of hydrophobicity, autoaggregation, EPS negative phenotype and were identified as Enterococcus hirae and Pediococcus pentosaceus. Nine LAB strains inhibited three indicator bacteria. Some isolates were pre-selected and genetically identified according to the results obtained. Antibiotic resistance and virulence factors were studied for the assessment of the safety of the strains. The results obtained were compared to those reported previously from samples obtained in the North-western area of the country and some differences were found.
authors.affiliation: Departamento de Microbiología Preventiva, CERELA-CONICET (Centro de Referencia para Lactobacilos-Consejo Nacional de Investigaciones Científicas y Técnicas), Chacabuco 145, 4000 San Miguel de Tucumán, Argentina.
authors.forename: M Carolina
authors.initials: MC
authors.lastname: Espeche
authors.forename: Matías
authors.initials: M
authors.lastname: Pellegrino
authors.forename: Ignacio
authors.initials: I
authors.lastname: Frola
authors.forename: Alejandro
authors.initials: A
authors.lastname: Larriestra
authors.forename: Cristina
authors.initials: C
authors.lastname: Bogni
authors.forename: M E Fátima
authors.initials: ME
authors.lastname: Nader-Macías
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000898:Antibiosis; D002417:Cattle; D024881:Drug Resistance, Bacterial; D005260:Female; D007777:Lactobacillaceae / Q000187:drug effects / Q000302:isolation & purification* / Q000502:physiology*; D008414:Mastitis, Bovine / Q000517:prevention & control*; D055442:Metabolome; D054892:Metagenome; D008826:Microbial Sensitivity Tests; D008892:Milk / Q000382:microbiology*; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis; D037521:Virulence Factors
chemical_list: D000900:Anti-Bacterial Agents; D011135:Polysaccharides, Bacterial; D037521:Virulence Factors