title: Rapid impedance measurement of tethered bilayer lipid membrane biosensors.
abstract: Tethered bilayer lipid membranes (tBLM) offer a promising means to immobilize membrane proteins for sensor applications and study biological phenomena including membrane-nanoparticle interactions. tBLM biointerfaces are typically characterized using electrochemical impedance spectroscopy (EIS) in the 1 mHz to 1 Hz range due to interface parasitics. To enable rapid characterization of biointerfaces for high throughput applications, this paper introduces a method for high resolution EIS characterization of tBLMs at higher frequencies. The tBLM equivalent electrical model is analyzed, and the benefit of extracting the real portion of interface admittance is described. Mathematical analysis shows that the maximum frequency for measuring membrane resistance is a function of membrane characteristics and that small area membranes could enable measurement well into the kHz range, permitting observation of millisecond membrane protein activity in biosensor arrays.
authors.affiliation: Electrical and Computer Engineering, Michigan State Univ., East Lansing, MI, USA.
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Mu
authors.forename: Daniel
authors.initials: D
authors.lastname: Rairigh
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Liu
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Mason
mesh_terms: D015374:Biosensing Techniques; D058266:Dielectric Spectroscopy; D008051:Lipid Bilayers
chemical_list: D008051:Lipid Bilayers