title: Rapid typing of extended-spectrum β-lactamase- and carbapenemase-producing Escherichia coli and Klebsiella pneumoniae isolates by use of SpectraCell RA.
abstract: Enterobacteriaceae are important pathogens of both nosocomial and community-acquired infections. In particular, strains with broad-spectrum beta-lactamases increasingly cause problems in health care settings. Rapid and reliable typing systems are key tools to identify transmission, so that targeted infection control measures can be taken. In this study, we evaluated the performance of Raman spectroscopic analysis (RA) for the typing of multiresistant Escherichia coli and Klebsiella pneumoniae isolates using the SpectraCell RA bacterial strain analyzer (River Diagnostics). Analysis of 96 unrelated isolates revealed that RA generated highly reproducible spectra and exhibited a discriminatory power that is comparable to pulsed-field gel electrophoresis. Furthermore, adequate results were obtained for three collections of clinical isolates. RA was able to discriminate outbreak-related isolates from isolates that were not involved in an outbreak or transmission. Furthermore, it was found that the RA approach recognized clones, irrespective of the extended-spectrum β-lactamase type. It can be concluded that RA is a suitable typing technique for E. coli and K. pneumoniae isolates. Combining high reproducibility, speed, and ease-of-use, this technique may play an important role in monitoring the epidemiology of these important nosocomial species.
authors.affiliation: Department of Medical Microbiology and Infectious Diseases, Erasmus MC, University Medical Center, Rotterdam, Netherlands. h.willemse@erasmusmc.nl
authors.forename: Diana
authors.initials: D
authors.lastname: Willemse-Erix
authors.forename: Tom
authors.initials: T
authors.lastname: Bakker-Schut
authors.forename: Femke
authors.initials: F
authors.lastname: Slagboom-Bax
authors.forename: Jan-willem
authors.initials: JW
authors.lastname: Jachtenberg
authors.forename: Nicole
authors.initials: N
authors.lastname: Lemmens-den Toom
authors.forename: Costas C
authors.initials: CC
authors.lastname: Papagiannitsis
authors.forename: Kuntaman
authors.initials: K
authors.lastname: Kuntaman
authors.forename: Gerwin
authors.initials: G
authors.lastname: Puppels
authors.forename: Alex
authors.initials: A
authors.lastname: van Belkum
authors.forename: Juliëtte A
authors.initials: JA
authors.lastname: Severin
authors.forename: Wil
authors.initials: W
authors.lastname: Goessens
authors.forename: Kees
authors.initials: K
authors.lastname: Maquelin
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D003428:Cross Infection; D024901:Drug Resistance, Multiple, Bacterial; D004926:Escherichia coli / Q000201:enzymology* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology; D006115:Greece / Q000453:epidemiology; D006801:Humans; D007710:Klebsiella Infections / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000201:enzymology* / Q000302:isolation & purification; D058889:Molecular Typing / Q000379:methods*; D009426:Netherlands / Q000453:epidemiology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013059:Spectrum Analysis, Raman; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases; C063912:carbapenemase