title: Metabolomics as a powerful tool for molecular quality assessment of the fish Sparus aurata.
abstract: The molecular profiles of perchloric acid solutions extracted from the flesh of Sparus aurata fish specimens, produced according to different aquaculture systems, have been investigated. The (1)H-NMR spectra of aqueous extracts are indicative of differences in the metabolite content of fish reared under different conditions that are already distinguishable at their capture, and substantially maintain the same differences in their molecular profiles after sixteen days of storage under ice. The fish metabolic profiles are studied by top-down chemometric analysis. The results of this exploratory investigation show that the fish metabolome accurately reflects the rearing conditions. The level of many metabolites co-vary with the rearing conditions and a few metabolites are quantified including glycogen (stress indicator), histidine, alanine and glycine which all display significant changes dependent on the aquaculture system and on the storage times.
authors.affiliation: Department of Food Science, University of Bologna, Piazza Goidanich 60, 47023 Cesena, Italy. gianfranco.picone2@unibo.it
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Picone
authors.forename: Søren Balling
authors.initials: SB
authors.lastname: Engelsen
authors.forename: Francesco
authors.initials: F
authors.lastname: Savorani
authors.forename: Silvia
authors.initials: S
authors.lastname: Testi
authors.forename: Anna
authors.initials: A
authors.lastname: Badiani
authors.forename: Francesco
authors.initials: F
authors.lastname: Capozzi
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D017756:Aquaculture / Q000379:methods*; D061353:Food Storage / Q000379:methods*; D005615:Freezing; D006003:Glycogen / Q000378:metabolism; D007053:Ice; D009682:Magnetic Resonance Spectroscopy; D055432:Metabolomics / Q000379:methods*; D018482:Muscle, Skeletal / Q000378:metabolism*; D021541:Sea Bream / Q000378:metabolism*; D017747:Seafood / Q000032:analysis*
chemical_list: D000596:Amino Acids; D007053:Ice; D006003:Glycogen