title: Oocyte mitochondrial bioenergy potential and oxidative stress: within-/between-subject, in vivo versus in vitro maturation, and age-related variations in a sheep model.
abstract: OBJECTIVE
  To analyze within-/between-subject, in vivo versus in vitro maturation (IVM), and age-related variations of mitochondrial (mt) bioenergy potential and oxidative status of metaphase II (MII) oocytes recovered from hormonally stimulated sheep.
  
  
  METHODS
  Prospective study.
  
  
  METHODS
  Academic basic research laboratory.
  
  
  METHODS
  Ten adult ewes.
  
  
  METHODS
  Estrus synchronization, controlled ovarian hyperstimulation (COH), ovariohysterectomy; follicular and oviductal oocyte retrieval; IVM of follicular oocytes.
  
  
  METHODS
  Mean ± SD, within-subject (CV(w)) and between-subject (CV(b)) variation coefficients of mt activity, intracellular reactive oxygen species (ROS) levels, and mt/ROS colocalization in sheep oocytes from young and aged donors and matured in vivo (in vivo MIIs) or in vitro (IVM MIIs).
  
  
  RESULTS
  Within- and between-subject, in vivo versus IVM, and age-related variations of mt activity were observed in MII oocytes from hormonally stimulated donor sheep. ROS levels increased significantly in oocytes from aged donors. Mt-ROS colocalization was consistently higher in in vivo MIIs compared with IVM MIIs. Oviductal energy/antioxidant ability is influenced by COH.
  
  
  CONCLUSIONS
  Oocyte energy/oxidative status is affected by within-/between-subject, in vivo versus IVM, and age-related variations. Mt/ROS colocalization is a reliable marker of in vivo MII oocytes.
authors.affiliation: Department of Animal Production, Aldo Moro University of Bari, Bari, Italy. nicmartino@libero.it
authors.forename: Nicola Antonio
authors.initials: NA
authors.lastname: Martino
authors.forename: Giovanni Michele
authors.initials: GM
authors.lastname: Lacalandra
authors.forename: Manuel
authors.initials: M
authors.lastname: Filioli Uranio
authors.forename: Barbara
authors.initials: B
authors.lastname: Ambruosi
authors.forename: Michele
authors.initials: M
authors.lastname: Caira
authors.forename: Fabio
authors.initials: F
authors.lastname: Silvestre
authors.forename: Flavia
authors.initials: F
authors.lastname: Pizzi
authors.forename: Salvatore
authors.initials: S
authors.lastname: Desantis
authors.forename: Gianluca
authors.initials: G
authors.lastname: Accogli
authors.forename: Maria Elena
authors.initials: ME
authors.lastname: Dell'Aquila
mesh_terms: D000367:Age Factors; D000375:Aging / Q000378:metabolism*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002478:Cells, Cultured; D004734:Energy Metabolism / Q000187:drug effects; D004973:Estrus Synchronization; D005260:Female; D005300:Fertility Agents, Female / Q000494:pharmacology; D059471:In Vitro Oocyte Maturation Techniques; D008677:Metaphase; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D054315:Oocyte Retrieval; D009865:Oocytes / Q000187:drug effects / Q000378:metabolism*; D010062:Ovulation Induction; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D012756:Sheep
chemical_list: D015415:Biomarkers; D005300:Fertility Agents, Female; D017382:Reactive Oxygen Species