title: Non-thermal dielectric barrier discharge plasma induces angiogenesis through reactive oxygen species.
abstract: Vascularization plays a key role in processes such as wound healing and tissue engineering. Non-thermal plasma, which primarily produces reactive oxygen species (ROS), recently emerged as an efficient tool in medical applications. Liquids and endothelial cells were treated with a non-thermal dielectric barrier discharge plasma. Plasma treatment of phosphate buffered saline (PBS) and serum-free medium increased ROS concentration in a dose-dependent manner, with a higher concentration in serum-free medium. ROS concentration in cells peaked 1 hour after treatment. 4.2 J/cm(2) increased cell proliferation, 2D and 3D migration, as well as tube formation. A fibroblast growth factor-2 (FGF-2) neutralizing antibody and ROS scavengers for hydrogen peroxide and hydroxyl radicals abrogated these angiogenic effects. Non-thermal plasma may be a potential tool for applying ROS in precise doses to enhance vascularization.
authors.affiliation: School of Biomedical Engineering, Science, & Health Systems, Drexel University, Philadelphia, PA 19104, USA.
authors.forename: Krishna P
authors.initials: KP
authors.lastname: Arjunan
authors.forename: Alisa Morss
authors.initials: AM
authors.lastname: Clyne
mesh_terms: D000818:Animals; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D018919:Neovascularization, Physiologic; D058626:Plasma Gases; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013552:Swine
chemical_list: D016895:Culture Media, Serum-Free; D058626:Plasma Gases; D017382:Reactive Oxygen Species