title: Isolation of mesotrione-degrading bacteria from aquatic environments in Brazil.
abstract: Mesotrione is a benzoylcyclohexane-1,3-dione herbicide that inhibits 4-hydroxyphenyl pyruvate dioxygenase in target plants. Although it has been used since 2000, only a limited number of degrading microorganisms have been reported. Mesotrione-degrading bacteria were selected among strains isolated from Brazilian aquatic environments, located near corn fields treated with this herbicide. Pantoea ananatis was found to rapidly and completely degrade mesotrione. Mesotrione did not serve as a sole C, N, or S source for growth of P. ananatis, and mesotrione catabolism required glucose supplementation to minimal media. LC-MS/MS analyses indicated that mesotrione degradation produced intermediates other than 2-amino-4-methylsulfonyl benzoic acid or 4-methylsulfonyl-2-nitrobenzoic acid, two metabolites previously identified in a mesotrione-degrading Bacillus strain. Since P. ananatis rapidly degraded mesotrione, this strain might be useful for bioremediation purposes.
authors.affiliation: Department of Structural Biology, Molecular Biology and Genetics, Microbiology Laboratory, Ponta Grossa State University, 84030-900 Ponta Grossa, PR, Brazil. marcos.pileggi@pq.cnpq.br
authors.forename: Marcos
authors.initials: M
authors.lastname: Pileggi
authors.forename: Sônia Alvim Veiga
authors.initials: SA
authors.lastname: Pileggi
authors.forename: Luiz Ricardo
authors.initials: LR
authors.lastname: Olchanheski
authors.forename: Paulo Augusto Garbugio
authors.initials: PA
authors.lastname: da Silva
authors.forename: Ana M
authors.initials: AM
authors.lastname: Munoz Gonzalez
authors.forename: Willian C
authors.initials: WC
authors.lastname: Koskinen
authors.forename: Brian
authors.initials: B
authors.lastname: Barber
authors.forename: Michael Jay
authors.initials: MJ
authors.lastname: Sadowsky
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D001938:Brazil; D003512:Cyclohexanones / Q000032:analysis / Q000378:metabolism*; D005618:Fresh Water / Q000737:chemistry / Q000382:microbiology*; D006540:Herbicides / Q000032:analysis / Q000378:metabolism*; D020636:Pantoea / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism*
chemical_list: D003512:Cyclohexanones; D006540:Herbicides; D014874:Water Pollutants, Chemical; C432907:mesotrione