title: Protein-RNA interactions: new genomic technologies and perspectives.
abstract: RNA-binding proteins are key players in the regulation of gene expression. In this Progress article, we discuss state-of-the-art technologies that can be used to study individual RNA-binding proteins or large complexes such as the ribosome. We also describe how these approaches can be used to study interactions with different types of RNAs, including nascent transcripts, mRNAs, microRNAs and ribosomal RNAs, in order to investigate transcription, RNA processing and translation. Finally, we highlight current challenges in data analysis and the future steps that are needed to obtain a quantitative and high-resolution picture of protein-RNA interactions on a genome-wide scale.
authors.affiliation: Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge                 CB2 0QH, UK.
authors.forename: Julian
authors.initials: J
authors.lastname: König
authors.forename: Kathi
authors.initials: K
authors.lastname: Zarnack
authors.forename: Nicholas M
authors.initials: NM
authors.lastname: Luscombe
authors.forename: Jernej
authors.initials: J
authors.lastname: Ule
mesh_terms: D000818:Animals; D019295:Computational Biology; D023281:Genomics / Q000379:methods*; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D012313:RNA / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000378:metabolism
chemical_list: D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; D012313:RNA