title: Control of active B and L cathepsins in tissues of colorectal cancer using cystatins isolated from chicken egg proteins: in vitro studies.
abstract: The activity of cysteine peptidases (cathepsins B and L) was estimated in homogenates of tissues sampled during surgery from 60 patients operated due to colorectal tumors. The results were compared to those obtained using tissues in which histopathology disclosed no tumorous cells, obtained from 20 patients of the same group, treated as a control. Activity of the enzymes was inhibited using cysteine peptidase inhibitors isolated from chicken egg proteins. Application of the inhibitors was found to inhibit activity of the enzymes which play a key role in tumor development. It is suggested that in future the inhibitors may provide a component of new generation drugs in the so-called inhibitor therapy.
authors.affiliation: Department of Surgery, Regional Hospital, Legnica, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Hap
authors.forename: Wojciech
authors.initials: W
authors.lastname: Kielan
authors.forename: Zygmunt
authors.initials: Z
authors.lastname: Grzebieniak
authors.forename: Maciej
authors.initials: M
authors.lastname: Siewinski
authors.forename: Jerzy
authors.initials: J
authors.lastname: Rudnicki
authors.forename: Robert
authors.initials: R
authors.lastname: Tarnawa
authors.forename: Julia
authors.initials: J
authors.lastname: Rudno-Rudzinska
authors.forename: Anil Kumar
authors.initials: AK
authors.lastname: Agrawal
mesh_terms: D000818:Animals; D002401:Cathepsin B / Q000037:antagonists & inhibitors* / Q000378:metabolism; D056668:Cathepsin L / Q000037:antagonists & inhibitors* / Q000378:metabolism; D002645:Chickens; D015179:Colorectal Neoplasms / Q000378:metabolism*; D015891:Cystatins / Q000494:pharmacology*; D015853:Cysteine Proteinase Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D004527:Egg Proteins / Q000737:chemistry; D006801:Humans
chemical_list: D015891:Cystatins; D015853:Cysteine Proteinase Inhibitors; D004527:Egg Proteins; D002401:Cathepsin B; D056668:Cathepsin L