title: A robust microfluidic in vitro cell perifusion system.
abstract: We present here a robust microfluidic cell perifusion device for in vitro primary tissue cell secretion studies. This system increases the sample concentration to perifusion volume ratio by an order of magnitude compared with standard multi-well plate static incubation assays. Further, this device achieves physiologically relevant flow rates, pressures, and temperature. It has been manufactured with typical machining facilities, principally drilling and milling. No specialist clean room equipment is required to replicate it. We show its capability here with hormone perifusion experiments on primary pancreatic tissue from mice. This device can increase cell secretion concentrations by up to a factor of 20, allowing for the first time the direct measurement of islet glucagon using mass spectrometry.
authors.affiliation: The Centre for Biologically-Inspired Technology, Bessemer Building, Level 4, Imperial College London, South Kensington Campus, London SW7 2AZ. christina.morris04@imperial.ac.uk
authors.forename: Christina
authors.initials: C
authors.lastname: Morris
authors.forename: Dylan J
authors.initials: DJ
authors.lastname: Banks
authors.forename: Lukasz
authors.initials: L
authors.lastname: Gaweda
authors.forename: Steve
authors.initials: S
authors.lastname: Scott
authors.forename: Xi Xi
authors.initials: XX
authors.lastname: Zhu
authors.forename: Maria
authors.initials: M
authors.lastname: Panico
authors.forename: Pantelis
authors.initials: P
authors.lastname: Georgiou
authors.forename: Christofer
authors.initials: C
authors.lastname: Toumazou
mesh_terms: D000818:Animals; D001681:Biological Assay; D005260:Female; D005934:Glucagon / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D044085:Microfluidics / Q000379:methods*; D010477:Perfusion / Q000379:methods*
chemical_list: D005934:Glucagon; D005947:Glucose