title: SMED-TLX-1 (NR2E1) is critical for tissue and body plan maintenance in Schmidtea mediterranea in fasting/feeding cycles.
abstract: Nuclear receptors (NRs), or nuclear hormone             receptors (NHRs), are transcription factors             that regulate development and metabolism of most if             not all animal species. Their regulatory networks include             conserved mechanisms that are shared in-between             species as well as mechanisms that are restricted             to certain phyla or even species. In search for             conserved members of the NHR family in Schmidtea             mediterranea, we identified a molecular signature of             a class of NRs, NR2E1, in the S. mediterranea genome             and cloned its complete cDNA coding sequence. The             derived amino acid sequence shows a high degree of             conservation of both DNA-binding domain and ligand-             binding domain and a remarkably high homology             to vertebrate NR2E1 and C. elegans NHR-67.             Quantitative PCR detected approximately ten-fold             higher expression of Smed-tlx-1 in the proximal part             of the head compared to the tail region. The expression             of Smed-tlx-1 is higher during fed state than             during fasting. Smed-tlx-1 down-regulation by RNA interference affects the ability of the animals to maintain             body plan and induces defects of brain, eyes and             body shape during fasting and re-growing cycles.             These results suggest that SMED-TLX-1 is critical             for tissue and body plan maintenance in planaria.
authors.affiliation: Laboratory of Model Systems, Institute of Inherited Metabolic Disorders, First Faculty of Medicine and General University Hospital, and Department of Normal, Pathological and Clinical Physiology, Charles University in Prague, Prague, Czech Republic.
authors.forename: O
authors.initials: O
authors.lastname: Raška
authors.forename: V
authors.initials: V
authors.lastname: Kostrouchová
authors.forename: F
authors.initials: F
authors.lastname: Behenský
authors.forename: P
authors.initials: P
authors.lastname: Yilma
authors.forename: V
authors.initials: V
authors.lastname: Saudek
authors.forename: Z
authors.initials: Z
authors.lastname: Kostrouch
authors.forename: M
authors.initials: M
authors.lastname: Kostrouchová
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019521:Body Patterning / Q000235:genetics; D003001:Cloning, Molecular; D005215:Fasting / Q000502:physiology*; D005247:Feeding Behavior / Q000502:physiology*; D005786:Gene Expression Regulation; D015801:Helminth Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D010802:Phylogeny; D034622:RNA Interference; D018160:Receptors, Cytoplasmic and Nuclear / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D014419:Turbellaria / Q000196:embryology* / Q000235:genetics / Q000502:physiology*
chemical_list: D015801:Helminth Proteins; D018160:Receptors, Cytoplasmic and Nuclear