title: Identification of the putative specific pathogenic genes of Porphyromonas gingivalis with type II fimbriae.
abstract: Porphyromonas gingivalis, the key etiologic agent of periodontitis, can be classified into six types (I to V and Ib) based on the fimA genes that encode FimA (a subunit of fimbriae). Accumulated evidence indicates that P. gingivalis expressing Type II fimbriae (Pg-II) is the most frequent isolate from severe periodontitis cases and is more virulent than other types of P. gingivalis. However, during the Pg-II infection process, which specific virulence factors play the key role is still unclear. In this study, we examined the capabilities of three Pg-II strains to invade and modulate the inflammatory cytokine expression of human gingival epithelial cells (GECs) compared to two Pg-I strains. P. gingivalis oligo microarrays were used to compare gene expression profiles of Pg-II strains that invade GECs with Pg-I strains. The differential gene expression of Pg-II was confirmed by quantitative reverse transcription-polymerase chain reaction. Our results showed that all of the Pg-II strains could induce interleukin (IL)-1β and IL-6 secretion significantly when compared to Pg-I strains. Thirty-seven genes that were specifically expressed during the pathogenic process of Pg-II were identified by a microarray assay. These findings provide a new insight at the molecular level to explain the specific pathogenic mechanism of Pg-II strains.
authors.affiliation: State Key Laboratory of Oral Diseases, West China College of Stomatology, Chengdu, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Gao
authors.forename: Yi
authors.initials: Y
authors.lastname: Xu
authors.forename: Shu
authors.initials: S
authors.lastname: Meng
authors.forename: Yafei
authors.initials: Y
authors.lastname: Wu
authors.forename: Haiyun
authors.initials: H
authors.lastname: Huang
authors.forename: Ruiying
authors.initials: R
authors.lastname: Su
authors.forename: Lei
authors.initials: L
authors.lastname: Zhao
mesh_terms: D000328:Adult; D000942:Antigens, Bacterial / Q000235:genetics*; D001422:Bacterial Adhesion / Q000235:genetics; D004847:Epithelial Cells / Q000378:metabolism / Q000382:microbiology; D020869:Gene Expression Profiling; D060005:Genotyping Techniques; D005881:Gingiva / Q000166:cytology / Q000473:pathology; D006801:Humans; D053583:Interleukin-1beta / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D008565:Membrane Proteins / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D010510:Periodontal Diseases / Q000382:microbiology; D016966:Porphyromonas gingivalis / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D013045:Species Specificity
chemical_list: D000942:Antigens, Bacterial; D053583:Interleukin-1beta; D015850:Interleukin-6; D008565:Membrane Proteins; C118696:fibA protein, bacteria