title: β-Amyloid peptide is internalized into chick retinal neurons and alters the distribution of myosin Vb.
abstract: The most common neurodegenerative disorder afflicting the aging human population is Alzheimer's disease (AD). A major hallmark of AD is dementia from a loss of neuronal function, attributed to the presence and accumulation of β-amyloid (Aβ) peptide into senile plaques. Preceding senile plaque formation, abnormalities in axons can be observed as changes in morphologies and intracellular trafficking. Recently, it has been recognized that Aβ also accumulates within neurons and this intraneuronal Aβ accumulation has been reported to be critical in the disruption of synapses and cognitive function. Here, we report on the internalization of a fluorescently labeled Aβ peptide into cultured chick retinal neurons. The pattern of Aβ distribution during the time course of incubation is reminiscent of the endocytic pathway. Furthermore, the distribution of the internalized Aβ peptide converges with that of myosin Vb and both relocalize from the axon to cell body. These observations are consistent with the hypothesis that AD proceeds as a result of an imbalance between Aβ production and Aβ clearance, suggesting a role for myosin Vb in this process.
authors.affiliation: Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Leandro T
authors.initials: LT
authors.lastname: Oliveira
authors.forename: Priscila A
authors.initials: PA
authors.lastname: Matos
authors.forename: David William
authors.initials: DW
authors.lastname: Provance
authors.forename: Fernando G
authors.initials: FG
authors.lastname: de Mello
authors.forename: Leonardo R
authors.initials: LR
authors.lastname: Andrade
authors.forename: Martha M
authors.initials: MM
authors.lastname: Sorenson
authors.forename: Verônica P
authors.initials: VP
authors.lastname: Salerno
mesh_terms: D016229:Amyloid beta-Peptides / Q000378:metabolism*; D000818:Animals; D001369:Axons / Q000378:metabolism*; D002470:Cell Survival; D002478:Cells, Cultured; D002642:Chick Embryo; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007124:Immunoenzyme Techniques; D018995:Myosin Heavy Chains / Q000378:metabolism*; D024701:Myosin Type V / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D012160:Retina / Q000166:cytology / Q000378:metabolism*; D013569:Synapses / Q000378:metabolism*
chemical_list: D016229:Amyloid beta-Peptides; C505832:MYO5B protein, human; D024701:Myosin Type V; D018995:Myosin Heavy Chains