title: Efficient double-strand scission of plasmid DNA by quaternized-chitosan zinc complex.
abstract: N-[(2-Hydroxy-3-trimethylammonium) propyl] chitosan chloride (HTACC) was prepared to construct a chitosan-based zinc complex (HTACC-Zn(II)) as a catalyst with good water solubility for rapid DNA cleavage. Results indicated that the observed rate constant (k(obs)) of plasmid DNA cleaved by HTACC-Zn(II) could be enhanced by 10(7)-fold compared with that of uncatalyzed DNA cleavage. The kinetic behavior of HTACC-Zn(II) for DNA cleavage is well fitted by Michaelis-Menten model. The results of gel electrophoresis suggested that HTACC-Zn(II) preferentially perform double-strand break of plasmid DNA.
authors.affiliation: School of Materials Science and Engineering, Beihang University, Haidian District, Beijing, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Xiang
authors.forename: Dawei
authors.initials: D
authors.lastname: Liang
authors.forename: Yinggui
authors.initials: Y
authors.lastname: Peng
authors.forename: Hong
authors.initials: H
authors.lastname: Guo
mesh_terms: D002384:Catalysis; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D053837:DNA Cleavage; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D008958:Models, Molecular; D009942:Organometallic Compounds / Q000737:chemistry*; D010957:Plasmids / Q000737:chemistry*; D012995:Solubility; D014867:Water / Q000737:chemistry*; D015032:Zinc / Q000737:chemistry*
chemical_list: C572320:N-((2-hydroxy-3-trimethylammonium)propyl)chitosan chloride; D009942:Organometallic Compounds; D014867:Water; D004247:DNA; D048271:Chitosan; D015032:Zinc