title: ANCUT2, an extracellular cutinase from Aspergillus nidulans induced by olive oil.
abstract: Cutinases are versatile carboxylic ester hydrolases with great potential in many biocatalytic processes, including biodiesel production. Genome sequence analysis of the model organism Aspergillus nidulans reveals four genes encoding putative cutinases. In this work, we purified and identified for the first time a cutinase (ANCUT2) produced by A. nidulans. ANCUT2 is a 29-kDa protein which consists of 255 amino acid residues. Comparison of the amino acid sequence of ANCUT2 with other microbial cutinase sequences revealed a high degree of homology with other fungal cutinases as well as new features, which include a serine-rich region and conserved cysteines. Cutinase production with different lipidic and carbon sources was also explored. Enzyme activity was induced by olive oil and some triacylglycerides and fatty acids, whereas it was repressed by glucose (1%) and other sugars. In some conditions, a 22-kDa post-translational processing product was also detected. The cutinase nature of the enzyme was confirmed after degradation of apple cutin.
authors.affiliation: Department of Food Science and Biotechnology, Universidad Nacional Autónoma de México (UNAM), Ciudad Universitaria, Mexico City, Mexico.
authors.forename: Denise
authors.initials: D
authors.lastname: Castro-Ochoa
authors.forename: Carolina
authors.initials: C
authors.lastname: Peña-Montes
authors.forename: Augusto
authors.initials: A
authors.lastname: González-Canto
authors.forename: Ana
authors.initials: A
authors.lastname: Alva-Gasca
authors.forename: Ricardo
authors.initials: R
authors.lastname: Esquivel-Bautista
authors.forename: Arturo
authors.initials: A
authors.lastname: Navarro-Ocaña
authors.forename: Amelia
authors.initials: A
authors.lastname: Farrés
mesh_terms: D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000166:cytology* / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D002244:Carbon / Q000494:pharmacology; D002265:Carboxylic Ester Hydrolases / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D003470:Culture Media; D019143:Evolution, Molecular; D005110:Extracellular Space / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D005227:Fatty Acids / Q000494:pharmacology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009584:Nitrogen / Q000494:pharmacology; D000069463:Olive Oil; D010802:Phylogeny; D010938:Plant Oils / Q000494:pharmacology*; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D014280:Triglycerides / Q000494:pharmacology
chemical_list: D003470:Culture Media; D005227:Fatty Acids; D000069463:Olive Oil; D010938:Plant Oils; D014280:Triglycerides; D002244:Carbon; D002265:Carboxylic Ester Hydrolases; C019952:cutinase; D009584:Nitrogen