title: Characterization of D-lactate dehydrogenase from Pediococcus acidilactici that converts phenylpyruvic acid into phenyllactic acid.
abstract: The gene coding for D-lactate dehydrogenase (D-LDH) from Pediococcus acidilactici DSM 20284 was cloned and expressed in E. coli. The recombinant enzyme was purified by nickel-affinity chromatography. It converted phenylpyruvic acid (PPA) to 3-phenyllactic acid maximally at 30°C and pH 5.5 with a specific activity of 140 and 422 U/mg for PPA and pyruvate, respectively. The K(m), turnover number (k(cat)), and catalytic efficiency (k(cat)/K(m)) for PPA were 2.9 mM, 305 s(-1), and 105 mM(-1) s(-1), respectively.
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, Jiangsu, China. wmmu@jiangnan.edu.cn
authors.forename: Wanmeng
authors.initials: W
authors.lastname: Mu
authors.forename: Shuhuai
authors.initials: S
authors.lastname: Yu
authors.forename: Bo
authors.initials: B
authors.lastname: Jiang
authors.forename: Xingfeng
authors.initials: X
authors.lastname: Li
mesh_terms: D000818:Animals; D001711:Biotransformation; D002415:Cats; D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D004795:Enzyme Stability; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D015870:Gene Expression; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D045022:Lactate Dehydrogenases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D007773:Lactates / Q000378:metabolism*; D010376:Pediococcus / Q000201:enzymology* / Q000235:genetics*; D010667:Phenylpyruvic Acids / Q000378:metabolism*; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D013696:Temperature
chemical_list: D007773:Lactates; D010667:Phenylpyruvic Acids; D011994:Recombinant Proteins; C017648:3-phenyllactic acid; D045022:Lactate Dehydrogenases; C071195:D-lactate dehydrogenase; C031606:phenylpyruvic acid