title: Ratiometric detection of Cr3+ and Hg2+ by a naphthalimide-rhodamine based fluorescent probe.
abstract: Newly synthesized rhodamine derivatives, L(1) and L(2), are found to bind specifically to Hg(2+) or Cr(3+) in presence of large excess of other competing ions with associated changes in their optical and fluorescence spectral behavior. These spectral changes are significant enough in the visible region of the spectrum and thus, allow the visual detection. For L(1), the detection limit is even lower than the permissible [Cr(3+)] or [Hg(2+)] in drinking water as per standard U.S. EPA norms; while the receptor, L2 could be used as a ratiometric sensor for detection of Cr(3+) and Hg(2+) based on the resonance energy transfer (RET) process involving the donor naphthalimide and the acceptor Cr(3+)/Hg(2+)-bound xanthene fragment. Studies reveal that these two reagents could be used for recognition and sensing of Hg(2+)/Cr(3+). Further, confocal laser microscopic studies confirmed that the reagent L(2) could also be used as an imaging probe for detection of uptake of these ions in A431 cells.
authors.affiliation: Central Salt and Marine Chemicals Research Institute (CSIR), Bhavnagar-364002, Gujarat, India.
authors.forename: Prasenjit
authors.initials: P
authors.lastname: Mahato
authors.forename: Sukdeb
authors.initials: S
authors.lastname: Saha
authors.forename: E
authors.initials: E
authors.lastname: Suresh
authors.forename: Rosa
authors.initials: R
authors.lastname: Di Liddo
authors.forename: Pier Paolo
authors.initials: PP
authors.lastname: Parnigotto
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Conconi
authors.forename: Manoj K
authors.initials: MK
authors.lastname: Kesharwani
authors.forename: Bishwajit
authors.initials: B
authors.lastname: Ganguly
authors.forename: Amitava
authors.initials: A
authors.lastname: Das
mesh_terms: D002460:Cell Line; D002857:Chromium / Q000032:analysis*; D005456:Fluorescent Dyes; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008628:Mercury / Q000032:analysis*; D018613:Microscopy, Confocal; D053644:Naphthalimides / Q000737:chemistry*; D011874:Radiometry; D012235:Rhodamines / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D005456:Fluorescent Dyes; D053644:Naphthalimides; D012235:Rhodamines; D002857:Chromium; D008628:Mercury