title: An egg-enriched diet attenuates plasma lipids and mediates cholesterol metabolism of high-cholesterol fed rats.
abstract: We investigated the influence of an egg-enriched diet on plasma, hepatic and fecal lipid levels and on gene expression levels of transporters, receptors and enzymes involved in cholesterol metabolism. Sprague-Dawley rats fed an egg-enriched diet had lower plasma triglycerides, total cholesterol, low density lipoprotein (LDL)-cholesterol, hepatic triglyceride, and cholesterol concentrations, and greater plasma high-density lipoprotein cholesterol concentration, fecal neutral sterol and bile acid concentrations than those fed a plain cholesterol diet. Chicken egg yolk had no effect on sterol 12α-hydroxylase and sterol 27α-hydroxylase; but upregulated mRNA levels of hepatic LDL-receptor, cholesterol 7α-hydroxylase (CYP7A1) and lecithin cholesterol acyltransferase, and downregulated hepatic hydroxymethylglutaryl-(HMG)-CoA reductase and acyl-CoA:cholesterol acyltransferase (ACAT) after 90 days. Modification of the lipoprotein profile by an egg-enriched diet was mediated by reducing de novo cholesterol synthesis and enhancing the excretion of fecal cholesterol, via upregulation of CYP7A1 and the LDL receptor, and downregulation of HMG-CoA reductase and ACAT.
authors.affiliation: National R&D Center for Egg Processing, College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, People's Republic of China.
authors.forename: Fang
authors.initials: F
authors.lastname: Yang
authors.forename: Meihu
authors.initials: M
authors.lastname: Ma
authors.forename: Jia
authors.initials: J
authors.lastname: Xu
authors.forename: Xiufang
authors.initials: X
authors.lastname: Yu
authors.forename: Ning
authors.initials: N
authors.lastname: Qiu
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000378:metabolism*; D002790:Cholesterol 7-alpha-Hydroxylase / Q000235:genetics / Q000378:metabolism; D004032:Diet; D004531:Eggs; D008055:Lipids / Q000097:blood; D008099:Liver / Q000201:enzymology / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D011973:Receptors, LDL / Q000235:genetics / Q000378:metabolism; D002785:Sterol O-Acyltransferase / Q000235:genetics / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism
chemical_list: D008055:Lipids; D011973:Receptors, LDL; D014280:Triglycerides; D002784:Cholesterol; C532838:CYP7A1 protein, rat; D002790:Cholesterol 7-alpha-Hydroxylase; D002785:Sterol O-Acyltransferase