title: Dead-end hollow-fiber ultrafiltration for concentration and enumeration of Escherichia coli and broad-host-range plasmid DNA from wastewater.
abstract: Broad-host-range plasmids can facilitate dissemination of antibiotic resistance determinants among diverse bacterial populations. We evaluated hollow-fiber ultrafiltration for increases in detection efficiency of broad-host-range plasmids and Escherichia coli DNA in wastewater. Ultrafiltration followed by PCR showed limited increases in DNA detection and quantification in effluent compared with membrane filtration alone.
authors.affiliation: Department of Crop, Soil, and Environmental Sciences, 115 Plant Science Building, University of Arkansas, Fayetteville, Arkansas 72701, USA.
authors.forename: Kyle L
authors.initials: KL
authors.lastname: Asfahl
authors.forename: Mary C
authors.initials: MC
authors.lastname: Savin
mesh_terms: D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification*; D004926:Escherichia coli / Q000235:genetics*; D010957:Plasmids / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014462:Ultrafiltration / Q000379:methods*
chemical_list: D004269:DNA, Bacterial