title: In vitro and in vivo study of cucurbitacins-type triterpene glucoside from Citrullus colocynthis growing in Saudi Arabia against hepatocellular carcinoma.
abstract: Chromatographic investigation of fruits obtained from Citrullus colocynthis, growing in Saudi Arabia, led to isolation of two compounds; Cucurbitacin E glucoside (Cu E, 1), and Cucurbitacin I glucoside (Cu I, 2). The chemical structures of 1 and 2, were elucidated by spectroscopic analyses include; 1D ((1)H and (13)C) and 2D (COSY, HMQC and HMBC) NMR and ESI-MS spectroscopy. The in vitro cytotoxic activity against hepatoma cell line (HepG2) and mice-bearing tumor of Ehrlich's ascites carcinoma (EAC) of the compounds were estimated. Both compounds had potent inhibitory activity on HepG2 with IC(50) 3.5 and 2.8 nmol/mL, respectively. In addition to these activities, the in vivo study employing EAC, showed the capability of both compounds to prolong the survival time, life span and normalize the biochemical parameters of the infected mice with EAC.
authors.affiliation: Department of Chemistry, Faculty of Science, King Abdulaziz University, PO Box 80203, Jeddah 21589, Saudi Arabia.
authors.forename: Seif-Eldin N
authors.initials: SE
authors.lastname: Ayyad
authors.forename: Ahmed
authors.initials: A
authors.lastname: Abdel-Lateff
authors.forename: Walied M
authors.initials: WM
authors.lastname: Alarif
authors.forename: Francesca R
authors.initials: FR
authors.lastname: Patacchioli
authors.forename: Farid A
authors.initials: FA
authors.lastname: Badria
authors.forename: Saleh T
authors.initials: ST
authors.lastname: Ezmirly
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D002286:Carcinoma, Ehrlich Tumor / Q000188:drug therapy* / Q000473:pathology; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D049109:Cell Proliferation / Q000187:drug effects; D002855:Chromatography, Thin Layer; D029773:Citrullus / Q000737:chemistry; D054728:Cucurbitacins / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005638:Fruit; D056945:Hep G2 Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D008113:Liver Neoplasms / Q000473:pathology*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051379:Mice; D015394:Molecular Structure; D010946:Plants, Medicinal; D012529:Saudi Arabia; D021241:Spectrometry, Mass, Electrospray Ionization; D013997:Time Factors; D014315:Triterpenes / Q000494:pharmacology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D014315:Triterpenes; D054728:Cucurbitacins; C038106:cucurbitacin I; C102326:cucurbitacin E