title: Novel cyanine dyes with vinylsulfone group for labeling biomolecules.
abstract: Novel vinylsulfone cyanine dyes (em. 550-850 nm) were designed and synthesized for fluorescent labeling of biomolecules via 1,2-addition reaction in aqueous conditions. Due to the virtue of chemical structures of both fluorophore and reactive group, these dyes could be significantly stable and reactive in various aqueous/organic conditions. A wide variety of pH, temperature, buffer concentration, and protein were tested for the optimal labeling condition.
authors.affiliation: Center for Neuro-Medicine, Brain Science Institute, Korea Institute of Science and Technology, L2317, 39-1 Hawolgok-dong, Sungbuk-ku, Seoul, Korea 136-791.
authors.forename: Jin Woo
authors.initials: JW
authors.lastname: Park
authors.forename: YoungSoo
authors.initials: Y
authors.lastname: Kim
authors.forename: Kee-Jung
authors.initials: KJ
authors.lastname: Lee
authors.forename: Dong Jin
authors.initials: DJ
authors.lastname: Kim
mesh_terms: D002853:Chromatography, Liquid; D004396:Coloring Agents / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D011506:Proteins / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013450:Sulfones / Q000737:chemistry*; D013696:Temperature
chemical_list: D004396:Coloring Agents; D011506:Proteins; D013450:Sulfones; C009873:divinyl sulfone