title: Recognition of different DNA sequences by a DNA-binding protein alters protein dynamics differentially.
abstract: λ-Repressor-operator sites interaction, particularly O(R)1 and O(R)2, is a key component of the λ-genetic switch. FRET from the dansyl bound to the C-terminal domain of the protein, to the intercalated EtBr in the operator DNA indicates that the structure of the protein is more compact in the O(R)2 complex than in the O(R)1 complex. Fluorescence anisotropy reveals enhanced flexibility of the C-terminal domain of the repressor at fast timescales after complex formation with O(R)1. In contrast, O(R)2 bound repressor shows no significant enhancement of protein dynamics at these timescales. These differences are shown to be important for correct protein-protein interactions. Altered protein dynamics upon specific DNA sequence recognition may play important roles in assembly of regulatory proteins at the correct positions.
authors.affiliation: Department of Chemical, Biological & Macromolecular Sciences, S.N. Bose National Centre for Basic Sciences, Block JD, Sector III, Salt Lake, Kolkata 700 098, India.
authors.forename: Tanumoy
authors.initials: T
authors.lastname: Mondol
authors.forename: Subrata
authors.initials: S
authors.lastname: Batabyal
authors.forename: Abhishek
authors.initials: A
authors.lastname: Mazumder
authors.forename: Siddhartha
authors.initials: S
authors.lastname: Roy
authors.forename: Samir Kumar
authors.initials: SK
authors.lastname: Pal
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000235:genetics* / Q000378:metabolism*; D005454:Fluorescence Polarization; D031541:Fluorescence Resonance Energy Transfer; D008958:Models, Molecular; D011485:Protein Binding; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary; D012097:Repressor Proteins / Q000737:chemistry / Q000378:metabolism*; D054334:Viral Regulatory and Accessory Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D012097:Repressor Proteins; D054334:Viral Regulatory and Accessory Proteins; C016649:phage repressor proteins; D004247:DNA