title: Proteomic study of the mucin granulae in an intestinal goblet cell model.
abstract: Goblet cells specialize in producing and secreting mucus with its main component, mucins. An inducible goblet-like cell line was used for the purification of the mucus vesicles stored in these cells by density gradient ultracentrifugation, and their proteome was analyzed by nanoLC-MS and MS/MS. Although the density of these vesicles coincides with others, it was possible to reveal a number of proteins that after immunolocalization on colon tissue and functional analyses were likely to be linked to the MUC2 vesicles. Most of the proteins were associated with the vesicle membrane or their outer surface. The ATP6AP2, previously suggested to be associated with vesicular proton pumps, was colocalized with MUC2 without other V-ATPase proteins and, thus, probably has roles in mucin vesicle function yet to be discovered. FAM62B, known to be a calcium-sensitive protein involved in vesicle fusion, also colocalized with the MUC2 vesicles and is probably involved in unknown ways in the later events of the MUC2 vesicles and their secretion.
authors.affiliation: Department of Medical Biochemistry and Cell Biology, University of Gothenburg , Box 440, 40530 Gothenburg, Sweden.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Rodríguez-Piñeiro
authors.forename: Sjoerd
authors.initials: S
authors.lastname: van der Post
authors.forename: Malin E V
authors.initials: ME
authors.lastname: Johansson
authors.forename: Kristina A
authors.initials: KA
authors.lastname: Thomsson
authors.forename: Alexey I
authors.initials: AI
authors.lastname: Nesvizhskii
authors.forename: Gunnar C
authors.initials: GC
authors.lastname: Hansson
mesh_terms: D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D012809:Colon, Sigmoid / Q000166:cytology / Q000378:metabolism*; D020397:Goblet Cells / Q000378:metabolism*; D006801:Humans; D055262:Mucin-2 / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D010446:Peptide Fragments / Q000737:chemistry; D025341:Principal Component Analysis; D011485:Protein Binding; D025941:Protein Interaction Mapping; D021381:Protein Transport; D040901:Proteomics; D050683:R-SNARE Proteins / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000378:metabolism; D022142:Secretory Vesicles / Q000378:metabolism*; D050857:Synaptotagmins / Q000737:chemistry / Q000378:metabolism; D025262:Vacuolar Proton-Translocating ATPases / Q000737:chemistry / Q000378:metabolism; D020826:rab3A GTP-Binding Protein / Q000378:metabolism; D000093342:Prorenin Receptor
chemical_list: D055262:Mucin-2; D010446:Peptide Fragments; D050683:R-SNARE Proteins; D011956:Receptors, Cell Surface; D050857:Synaptotagmins; D025262:Vacuolar Proton-Translocating ATPases; D020826:rab3A GTP-Binding Protein; C458319:ATP6AP2 protein, human; D000093342:Prorenin Receptor; C573315:ESYT2 protein, human; C524625:MUC2 protein, human; C121241:VAMP7 protein, human; C112938:VAMP8 protein, human