title: MicroRNA-34a affects the occurrence of laryngeal squamous cell carcinoma by targeting the antiapoptotic gene survivin.
abstract: Survivin has been shown to be an ideal target for cancer gene therapy because of its strong antiapoptotic effect. MicroRNA-34a (miR-34a) can function as a tumor suppressor in some cancers through negative regulation of gene expression. However, the relationship between miR-34a and survivin in larynx squamous cell carcinoma (LSCC) has not been explored. The abundance of survivin mRNA and miR-34a in LSCC tissues were measured using quantitative real-time polymerase chain reaction. Their expression levels were analyzed and correlated with tumor differentiation, lymphatic metastasis, clinical stages, and survival rates. MiR-34a mimic was transfected using liposomes to increase its level in LSCC cancer cell line, Hep-2. The effects of miR-34a on survivin protein expression were tested using western blot analysis. Cell cycle analyses were performed using flow cytometry. The results showed that transfection of miR-34a mimic significantly suppressed cell proliferation with decreased survivin protein expression, but did not affect mRNA expression level. The results from LSCC tissue samples showed that miR-34a was downregulated, while survivin expression was upregulated. The miR-34a levels were negatively correlated with histologic differentiation and were positively correlated with survival rate. MiR-34a significantly suppressed cell proliferation by arresting cells at G0/G1 phase in Hep-2 cells. These results indicated that miR-34a may affect the occurrence of LSCC by targeting survivin.
authors.affiliation: Department of Otorhinolaryngology (Head and Neck Surgery), Lihuili Hospital of Ningbo University, Ningbo University School of Medicine, Ningbo, 315040, China. szs7216@sina.com
authors.forename: Zhisen
authors.initials: Z
authors.lastname: Shen
authors.forename: Guowen
authors.initials: G
authors.lastname: Zhan
authors.forename: Dong
authors.initials: D
authors.lastname: Ye
authors.forename: Yuan
authors.initials: Y
authors.lastname: Ren
authors.forename: Lixin
authors.initials: L
authors.lastname: Cheng
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Wu
authors.forename: Junming
authors.initials: J
authors.lastname: Guo
mesh_terms: D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D002453:Cell Cycle; D045744:Cell Line, Tumor; D018572:Disease-Free Survival; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics*; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D020935:MAP Kinase Signaling System; D035683:MicroRNAs / Q000502:physiology*; D008875:Middle Aged; D000077022:Survivin
chemical_list: C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; C522425:MIRN34 microRNA, human; D035683:MicroRNAs; D000077022:Survivin