title: HtrA2/Omi is involved in 6-OHDA-induced endoplasmic reticulum stress in SH-SY5Y cells.
abstract: Endoplasmic reticulum (ER) stress has been shown to be associated with the pathogenesis of neurodegenerative disorders including Parkinson's disease (PD). HtrA2/Omi, from its participation in protein quality control, is involved in ER stress. However, little is known about the relationship between HtrA2/Omi and ER stress in PD. Here, we explored the association of HtrA2/Omi and ER stress in a cell model of PD and found that the expression level of HtrA2/Omi decreased with ER stress induction in 6-OHDA-treated SH-SY5Y cells. Furthermore, silencing endogenous expression of HtrA2/Omi with siRNA resulted in aggregated ER stress and cell death. Taken together, our results show that HtrA2/Omi may exert a protective function in 6-OHDA-induced cell death by regulating ER stress-related proteins. This research offers some clues as why mutations in HtrA2/Omi lead to higher susceptibility in some PD patients.
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, People's Republic of China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Luo
authors.forename: Lei
authors.initials: L
authors.lastname: Wei
authors.forename: Congcong
authors.initials: C
authors.lastname: Sun
authors.forename: Xiaowu
authors.initials: X
authors.lastname: Chen
authors.forename: Tan
authors.initials: T
authors.lastname: Wang
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.forename: Zhuolin
authors.initials: Z
authors.lastname: Liu
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Chen
authors.forename: Pingyi
authors.initials: P
authors.lastname: Xu
mesh_terms: D045744:Cell Line, Tumor; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects* / Q000235:genetics*; D020022:Genetic Predisposition to Disease / Q000235:genetics; D000074923:High-Temperature Requirement A Serine Peptidase 2; D006801:Humans; D024101:Mitochondrial Proteins / Q000235:genetics / Q000502:physiology*; D009447:Neuroblastoma; D009474:Neurons / Q000473:pathology / Q000502:physiology; D016627:Oxidopamine / Q000633:toxicity*; D010300:Parkinson Disease / Q000235:genetics* / Q000473:pathology*; D012697:Serine Endopeptidases / Q000235:genetics / Q000502:physiology*; D013565:Sympatholytics / Q000633:toxicity
chemical_list: D024101:Mitochondrial Proteins; D013565:Sympatholytics; D016627:Oxidopamine; D012697:Serine Endopeptidases; C000615914:HTRA2 protein, human; D000074923:High-Temperature Requirement A Serine Peptidase 2