title: High yield bio-butanol production by solvent-producing bacterial microflora.
abstract: Highly efficient butanol-producing bacterial microflora were isolated from hydrogen-producing sludge of a sewage treatment plant. Based on denaturing gradient gel electrophoresis (DGGE) analysis and 16s rDNA comparison, four strains from the butanol-producing microflora were identified as Clostridium saccharoperbutylacetonicum, Clostridium butylicum, Clostridium beijernckii, and Clostridium acetobutylicum. The effects of glucose, FeSO(4) · 7H(2)O and yeast extract concentrations on the butanol production by the mixture culture were investigated on batch mode. The medium composition for bio-butanol production was optimized using the Box-Behnken design and response surface methodology (RSM). The maximum butanol production rate (0.25 ± 0.02 g/L-h) and concentration (12.4 g/L) were obtained under the condition of glucose concentration, 60 g/L; FeSO(4) · 7H(2)O, 0.516 g/L; yeast extract concentration, 5.13 g/L. Addition of 6.0 g/L butyric acid significantly increased the butanol titer to 17.51 ± 0.49 g/L. Pressurized fermentation strategy (employed with a 5L fermentor) further enhanced the butanol concentration to 21.35 g/L, along with a maximum butanol rate of 1.25 g/L-h.
authors.affiliation: Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Chieh-Lun
authors.initials: CL
authors.lastname: Cheng
authors.forename: Pei-Yi
authors.initials: PY
authors.lastname: Che
authors.forename: Bor-Yann
authors.initials: BY
authors.lastname: Chen
authors.forename: Wen-Jhy
authors.initials: WJ
authors.lastname: Lee
authors.forename: Liang-Jung
authors.initials: LJ
authors.lastname: Chien
authors.forename: Jo-Shu
authors.initials: JS
authors.lastname: Chang
mesh_terms: D000440:Butanols / Q000378:metabolism*; D003013:Clostridium / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D012997:Solvents / Q000378:metabolism*
chemical_list: D000440:Butanols; D012997:Solvents; D005947:Glucose