title: Use of negative dielectrophoresis for selective elution of protein-bound particles.
abstract: In this paper with the aid of negative dielectrophoresis force in conjunction with shear force and at an optimal sodium hydroxide concentration we demonstrated a switchlike functionality to elute specifically bound beads from the surface. At an optimal flow rate and sodium hydroxide concentration, negative dielectrophoresis turned on results in bead detachment, whereas when negative dielectrophoresis is off, the beads remain attached. This platform offers the potential for performing a bead-based multiplexed assay where in a single channel various regions are immobilized with a different antibody, each targeting a different antigen. To develop the proof of concept and to demonstrate the switchlike functionality in eluting specifically bound beads from the surface we looked at two different protein interactions. We chose interactions that were in the same order of magnitude in strength as typical antibody-antigen interactions. The first was protein G-IgG interaction, and the second was the interaction between anti-IgG and IgG.
authors.affiliation: Stanford Genome Technology Center, Stanford, California, USA. mehdij@stanford.edu
authors.forename: Mehdi
authors.initials: M
authors.lastname: Javanmard
authors.forename: Sam
authors.initials: S
authors.lastname: Emaminejad
authors.forename: Robert W
authors.initials: RW
authors.lastname: Dutton
authors.forename: Ronald W
authors.initials: RW
authors.lastname: Davis
mesh_terms: D000906:Antibodies / Q000276:immunology; D000936:Antigen-Antibody Complex / Q000737:chemistry; D001426:Bacterial Proteins / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D004586:Electrophoresis; D007074:Immunoglobulin G / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D011485:Protein Binding; D012972:Sodium Hydroxide / Q000737:chemistry
chemical_list: D000906:Antibodies; D000936:Antigen-Antibody Complex; D001426:Bacterial Proteins; C034284:IgG Fc-binding protein, Streptococcus; D007074:Immunoglobulin G; D012972:Sodium Hydroxide