title: Heterologous production of polyketides by modular type I polyketide synthases in Escherichia coli.
abstract: Heterologous production of polyketide compounds, an important class of natural products with complex chemical structures, was first demonstrated with Streptomyces parvulus in 1984. Although Streptomyces strains are good first options for heterologous polyketide biosynthesis, their slow growth kinetics prompt other hosts to also be considered. Escherichia coli provides key elements of an ideal host in terms of the growth rate, culture conditions, and available recombinant DNA tools. Here we review the current status and potential for metabolic engineering of polyketides in E. coli.
authors.affiliation: QB3 Institute, University of California, Berkeley, CA 94270, USA.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Yuzawa
authors.forename: Woncheol
authors.initials: W
authors.lastname: Kim
authors.forename: Leonard
authors.initials: L
authors.lastname: Katz
authors.forename: Jay D
authors.initials: JD
authors.lastname: Keasling
mesh_terms: D001688:Biological Products / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D048630:Polyketide Synthases / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism*; D061065:Polyketides / Q000378:metabolism*; D015202:Protein Engineering; D058615:Synthetic Biology / Q000379:methods
chemical_list: D001688:Biological Products; D061065:Polyketides; D048630:Polyketide Synthases