title: Hyperthermia worsens ischaemic brain injury through destruction of microvessels in an embolic model in rats.
abstract: OBJECTIVE
  Basal lamina is a major part of the microvascular wall and plays a critical role in the integrity of microvasculature. The aim of this study is to determine whether hyperthermia worsens the destruction of microvascular integrity in the ischaemic injured brain.
  
  
  METHODS
  Focal cerebral ischaemia was induced by embolising a pre-formed clot into the middle cerebral artery (MCA). Rats received either normothermic or hyperthermic treatment. Neurological score and infarct size were evaluated at 24 h after the MCA occlusion. Microvascular collagen type IV and laminin were measured with fluorescence microscopy. The activities of matrix metalloproteinases (MMP-2 and MMP-9) and plasminogen activators (tPA and uPA) were determined by zymography.
  
  
  RESULTS
  Treatment with hyperthermia significantly increased infarct volume (p<0.01), cortex swelling (p<0.01), striatum swelling (p<0.05) and neurologic score (p<0.01) at 24 h after the MCA occlusion. Compared to the normothermic groups, hyperthermia significantly worsened the losses of microvascular basal lamina structure proteins, collagen type IV and laminin, at 6 h (p<0.001) and 24 h (p<0.01) after MCA occlusion. Hyperthermia increased the MMP-9 activity at 6 and 24 h after MCA occlusion compared with normothermia (p<0.05), whereas increased the MMP-2 activity at 6 h only (p<0.05). Hyperthermia also elevated uPA activity significantly at 6 and 24 h after MCA occlusion compared to normothermia (p<0.05).
  
  
  CONCLUSIONS
  These results demonstrate that hyperthermia exacerbates the destruction of microvascular integrity possibly by increasing the activities of MMP-2, MMP-9 and uPA in the ischaemic cerebral tissues.
authors.affiliation: Department of Neurology, the First People's Hospital of Yunnan Province, Kunming, PR China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Meng
authors.forename: Chunyan
authors.initials: C
authors.lastname: He
authors.forename: Ashfaq
authors.initials: A
authors.lastname: Shuaib
authors.forename: Chen Xu
authors.initials: CX
authors.lastname: Wang
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000098:blood supply* / Q000473:pathology; D001522:Behavior, Animal; D020520:Brain Infarction / Q000473:pathology* / Q000503:physiopathology; D002545:Brain Ischemia / Q000473:pathology* / Q000503:physiopathology; D002540:Cerebral Cortex / Q000098:blood supply / Q000473:pathology; D024141:Collagen Type IV / Q000378:metabolism; D003342:Corpus Striatum / Q000098:blood supply / Q000473:pathology; D004195:Disease Models, Animal; D006979:Hyperthermia, Induced / Q000009:adverse effects*; D007797:Laminin / Q000378:metabolism; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D055806:Microvessels / Q000473:pathology*; D010960:Plasminogen Activators / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D024141:Collagen Type IV; D007797:Laminin; D010960:Plasminogen Activators; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9