title: [Two optimized transformation protocols for a cellulose-utilizing fungus Chaetomium globosum NK-102].
abstract: OBJECTIVE
  We developed the transformation methods of the strain Chaetomium globosum NK-102.
  
  
  METHODS
  We constructed plasmid pUCATPH-Pgap and compared the transformation efficiency with pUCATPH and pCM768. We established the PEG mediated protoplast transformation and Agrobacterium tumefaciens EHA105 mediated transformation methods.
  
  
  RESULTS
  In protoplast approach, approximately 3 -5 transformants/microg DNA could be obtained. The highest efficiency of transformation was obtained by employing pUCATPH-Pgap. A. tumefaciens EHA105 successfully mediated T-DNA insertion into the genome of C. globosum NK-102 and the transformation rate was 3.2 x 10(2) transformants/10(7) spores. The transformants retained stable after generations. Southern blot analyses confirmed that the DNA had integrated into the chromosomal DNA of C. globosum NK-102.
  
  
  CONCLUSIONS
  The transformation systems were good basis for selection of C. globosum mutant strains that effectively utilizing cellulose.
authors.affiliation: National Key Program of Microbiology, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin 300071, China. hxrr_563@163.com
authors.forename: Xiaoran
authors.initials: X
authors.lastname: Hao
authors.forename: Yuan
authors.initials: Y
authors.lastname: Ji
authors.forename: Huang
authors.initials: H
authors.lastname: Chen
authors.forename: Jiannan
authors.initials: J
authors.lastname: Bi
authors.forename: Jiao
authors.initials: J
authors.lastname: Pan
authors.forename: Xudong
authors.initials: X
authors.lastname: Zhu
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism*; D002597:Chaetomium / Q000235:genetics* / Q000378:metabolism*; D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial / Q000235:genetics; D016680:Genome, Bacterial; D010957:Plasmids / Q000235:genetics; D014170:Transformation, Genetic
chemical_list: D004269:DNA, Bacterial; D002482:Cellulose