title: Evaluation of a novel commercial rapid test for dengue diagnosis based on specific IgA detection.
abstract: The performance of the novel commercial test ASSURE® Dengue IgA Rapid test (MP Diagnostics) was evaluated using a panel of 172 sera collected from dengue patients and 47 sera from healthy blood donors. The overall specificity and sensitivity were 61.0% and 85.1%, respectively. However, the positivity rate for IgA went from 33.3% for sera collected the same day of fever onset to 81.2% for sera collected 5 days after fever onset. Infections with serotype 2 viruses were detected more efficiently than those with serotype 1 viruses, and no sera from infections with serotypes 3 and 4 were available. In addition, the kit was twice more efficient at detecting secondary infections than at detecting primary infections. Finally, the ASSURE® test showed good repeatability and reproducibility. The results of this study suggest that the ASSURE® Dengue IgA Rapid test may become a useful and easy-to-use test for early dengue diagnosis.
authors.affiliation: Departamento de Virología, Centro de Investigación y Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Mexico D.F., Mexico.
authors.forename: Sergio Isaac
authors.initials: SI
authors.lastname: de la Cruz Hernández
authors.forename: Silvia
authors.initials: S
authors.lastname: González Mateos
authors.forename: Hilario
authors.initials: H
authors.lastname: Flores Aguilar
authors.forename: Irma
authors.initials: I
authors.lastname: López Martinez
authors.forename: Celia
authors.initials: C
authors.lastname: Alpuche Aranda
authors.forename: Juan E
authors.initials: JE
authors.lastname: Ludert
authors.forename: Rosa María
authors.initials: RM
authors.lastname: Del Angel
mesh_terms: D000914:Antibodies, Viral / Q000097:blood*; D003715:Dengue / Q000097:blood* / Q000175:diagnosis*; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood*; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D000914:Antibodies, Viral; D007070:Immunoglobulin A; D011933:Reagent Kits, Diagnostic