title: Synthesis and secretion of renin in mice with induced genetic mutations.
abstract: The juxtaglomerular (JG) cell product renin is rate limiting in the generation of the bioactive octapeptide angiotensin II. Rates of synthesis and secretion of the aspartyl protease renin by JG cells are controlled by multiple afferent and efferent pathways originating in the CNS, cardiovascular system, and kidneys, and making critical contributions to the maintenance of extracellular fluid volume and arterial blood pressure. Since both excesses and deficits of angiotensin II have deleterious effects, it is not surprising that control of renin is secured by a complex system of feedforward and feedback relationships. Mice with genetic alterations have contributed to a better understanding of the networks controlling renin synthesis and secretion. Essential input for the setting of basal renin generation rates is provided by β-adrenergic receptors acting through cyclic adenosine monophosphate, the primary intracellular activation mechanism for renin mRNA generation. Other major control mechanisms include COX-2 and nNOS affecting renin through PGE2, PGI2, and nitric oxide. Angiotensin II provides strong negative feedback inhibition of renin synthesis, largely an indirect effect mediated by baroreceptor and macula densa inputs. Adenosine appears to be a dominant factor in the inhibitory arms of the baroreceptor and macula densa mechanisms. Targeted gene mutations have also shed light on a number of novel aspects related to renin processing and the regulation of renin synthesis and secretion.
authors.affiliation: National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA. jurgens@intra.niddk.nih.gov
authors.forename: Jurgen
authors.initials: J
authors.lastname: Schnermann
authors.forename: Josephine P
authors.initials: JP
authors.lastname: Briggs
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D017630:Connexins / Q000235:genetics / Q000378:metabolism; D025461:Feedback, Physiological; D005786:Gene Expression Regulation; D005838:Genotype; D007606:Juxtaglomerular Apparatus / Q000201:enzymology* / Q000378:metabolism*; D051379:Mice; D009154:Mutation; D019001:Nitric Oxide Synthase / Q000235:genetics / Q000378:metabolism; D010641:Phenotype; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics / Q000378:metabolism; D011941:Receptors, Adrenergic / Q000235:genetics / Q000378:metabolism; D011982:Receptors, Prostaglandin / Q000235:genetics / Q000378:metabolism; D018047:Receptors, Purinergic P1 / Q000235:genetics / Q000378:metabolism; D012083:Renin / Q000096:biosynthesis* / Q000378:metabolism*; D012084:Renin-Angiotensin System / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics
chemical_list: D017630:Connexins; D011941:Receptors, Adrenergic; D011982:Receptors, Prostaglandin; D018047:Receptors, Purinergic P1; D019001:Nitric Oxide Synthase; D011451:Prostaglandin-Endoperoxide Synthases; D012083:Renin