title: Case study: recovery of ancient nuclear DNA from toe pads of the extinct passenger pigeon.
abstract: A variety of DNA extraction methods have been employed successfully to extract DNA from museum specimens. Toe pads are a common source of ancient DNA in birds, as they are generally not an informative character and can be removed without significant destruction of precious specimens. However, the DNA in these tissues is often highly degraded, both by natural postmortem decay and due to treatment by preservatives. In this case study chapter, we describe the use of both a commercial DNA extraction method and a silica-based method to extract ancient DNA from desiccated toe pads from the extinct passenger pigeon, Ectopistes migratorius. Successful amplification of nuclear DNA was achieved from both methods, representing the first nuclear DNA sequence recovered from this extinct species. We describe simple modifications to both protocols that we employed during the DNA extraction process.
authors.affiliation: Department of Biology, The Pennsylvania State University, 320 Mueller Laboratory, University Park, PA 16802, USA. tlf19@psu.edu
authors.forename: Tara L
authors.initials: TL
authors.lastname: Fulton
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Wagner
authors.forename: Beth
authors.initials: B
authors.lastname: Shapiro
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000235:genetics; D010856:Columbidae / Q000235:genetics*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D004229:Dithiothreitol / Q000737:chemistry; D053476:Extinction, Biological; D010802:Phylogeny; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D012822:Silicon Dioxide; D004247:DNA; D004229:Dithiothreitol