title: Effect of PDGF-BB combined with EDTA gel on adhesion and proliferation to the root surface.
abstract: Periodontal regeneration using EDTA or PDGF showed promising results, but the effect of combined application was still unclear. This study aimed to verify the effect of EDTA and/or PDGF application on root adhesion and proliferation of PDL fibroblast cells. Eighty specimens were prepared from forty periodontitis teeth and made five groups: (1) diseased (untreated), (2) SRP (scaling root planing), (3) EDTA (24%), (4) PDGF (25 ng/ml) and (5) Combined application of EDTA and PDGF. Periodontal ligament cells were cultured on the above conditioned dentin plate, and SEM examination was preformed and cells were counted within a representative standard area for both cell morphology and density. All groups including untreated showed significantly increase of adhered cells from baseline to 7 days. Among them, rate of increase was much higher in EDTA, PDGF, and combined groups. ANOVA test indicated that the number of cells in PDGF and combined groups was significantly higher than diseased group at 1 day. On day 7, PDGF and combined groups showed significantly higher number of adhesion cells than that found in the diseased, SRP and EDTA groups. Thus, root conditioning with EDTA enhanced cell adhesion more than SRP alone. There was no significant difference of cell number between PDGF and combined group. Combined application of EDTA and PDGF increased significantly PDL cell adhesion than EDTA alone. PDGF alone, however, also showed comparable effect to combined application at all periods. Thus, synergistic effect between PDGF and EDTA was not observed.
authors.affiliation: Department of Oral Medicine, Periodontology & Oral Diagnosis, Faculty of Dentistry, El-Minia University, El-Minia, Egypt. mahmoud_672000@yahoo.com
authors.forename: Mahmoud Helmy
authors.initials: MH
authors.lastname: Belal
authors.forename: Hisashi
authors.initials: H
authors.lastname: Watanabe
authors.forename: Shizuko
authors.initials: S
authors.lastname: Ichinose
authors.forename: Isao
authors.initials: I
authors.lastname: Ishikawa
mesh_terms: D000328:Adult; D000368:Aged; D000077214:Becaplermin; D002448:Cell Adhesion / Q000187:drug effects; D002452:Cell Count; D018929:Cell Culture Techniques; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D012534:Dental Scaling / Q000379:methods; D003804:Dentin / Q000187:drug effects / Q000648:ultrastructure; D004338:Drug Combinations; D004492:Edetic Acid / Q000494:pharmacology*; D005260:Female; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D008297:Male; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged; D010513:Periodontal Ligament / Q000187:drug effects* / Q000473:pathology; D010518:Periodontitis / Q000473:pathology; D020574:Proto-Oncogene Proteins c-sis / Q000494:pharmacology*; D012038:Regeneration / Q000187:drug effects; D016745:Root Planing / Q000379:methods; D014092:Tooth Root / Q000187:drug effects* / Q000648:ultrastructure; D055815:Young Adult
chemical_list: D004338:Drug Combinations; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin; D004492:Edetic Acid