title: Histone H1 expression in human prostate cancer tissues and cell lines.
abstract: Histone H1, one of the histone superfamilies, is known to determine chromatin structure and alter gene expression. It also contributes to regulation of cell proliferation in breast cancer. We hypothesized a similar association in prostate cancer, and therefore examined relationships between histone H1 expression and Gleason pattern, Ki-67 and androgen receptor levels in a series of prostate cancer tissues and cell lines. Histone H1 positive cancer cells increased with the Gleason pattern. Gleason pattern 3 tumors were divided into two groups, one with high histone H1 positivity (H1-high cases, 60-100% positivity) and the other with low histone H1 positivity (H1-low cases, 0-20% positivity). Ki-67 or androgen receptor positivity in H1-high cases was significantly higher than in H1-low cases. PC3 cells demonstrated more frequent histone H1 and Ki-67 positivity as compared to LNCaP cells. Silencing of histone H1 by siRNA transfection significantly reduced cell proliferation in LNCaP and PC3. These findings suggest that histone H1 expression is associated with the Gleason pattern, cell proliferation and androgen receptor expression in prostate cancers.
authors.affiliation: Department of Experimental Pathology and Tumor Biology, Graduate School of Medical Sciences, Nagoya City University, Japan. satopin@med.nagoya-cu.ac.jp
authors.forename: Shinya
authors.initials: S
authors.lastname: Sato
authors.forename: Satoru
authors.initials: S
authors.lastname: Takahashi
authors.forename: Makoto
authors.initials: M
authors.lastname: Asamoto
authors.forename: Makoto
authors.initials: M
authors.lastname: Nakanishi
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Wakita
authors.forename: Yuji
authors.initials: Y
authors.lastname: Ogura
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Yatabe
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Shirai
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008297:Male; D060787:Neoplasm Grading; D011467:Prostate / Q000378:metabolism* / Q000473:pathology; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D011944:Receptors, Androgen / Q000235:genetics / Q000378:metabolism
chemical_list: D006657:Histones; D011944:Receptors, Androgen