title: Preparation and characterization of chitosan-carbon nanotube scaffolds for bone tissue engineering.
abstract: In recent years, significant development has been given to chitosan for orthopedic application. In this study, we have prepared scaffolds with the use of low and high molecular weight chitosan with 0.0025%, 0.005% and 0.01% weight of f-multiwalled carbon nanotube (f-MWCNT) by freezing and lyophilization method and physiochemically characterized as bone graft substitutes. Fourier Transform Infrared Spectroscopy, X-Ray Diffraction Analysis, Thermal Gravimetric Analysis, Scanning Electron Microscopy and Optical Microscopy results indicated that the f-MWCNT was uniformly dispersed in chitosan matrix and there was a chemical interaction between chitosan and f-MWCNT. The water uptake ability and porosity of scaffolds increased with an increase the amount of f-MWCNT. The cell proliferation, protein content, alkaline phosphatase and mineralization of the composite scaffolds were higher than chitosan scaffold due to the addition of f-MWCNT. Herewith, we are suggesting that chitosan/f-MWCNT scaffolds are promising biomaterials for bone tissue engineering.
authors.affiliation: Department of Chemistry, Pukyong National University, Busan, Republic of Korea.
authors.forename: Jayachandran
authors.initials: J
authors.lastname: Venkatesan
authors.forename: Bomi
authors.initials: B
authors.lastname: Ryu
authors.forename: P N
authors.initials: PN
authors.lastname: Sudha
authors.forename: Se-Kwon
authors.initials: SK
authors.lastname: Kim
mesh_terms: D018786:Bone Substitutes / Q000737:chemistry*; D002460:Cell Line; D048271:Chitosan / Q000737:chemistry*; D004055:Differential Thermal Analysis; D006801:Humans; D008422:Materials Testing; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D016062:Porosity; D013818:Thermogravimetry; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D018786:Bone Substitutes; D037742:Nanotubes, Carbon; D014867:Water; D048271:Chitosan