title: Global gene expression profiles in developing soybean seeds.
abstract: The gene expression profiles in soybean (Glycine max L.) seeds at 4 stages of development, namely, pod, 2-mm bean, 5-mm bean, and full-size bean, were examined by DNA microarray analysis. The total genes of each sample were classified into 4 clusters based on stage of development. Gene expression was strictly controlled by seed size, which coincides with the development stage. First, stage specific gene expression was examined. Many transcription factors were expressed in pod, 2-mm bean and 5-mm bean. In contrast, storage proteins were mainly expressed in full-size bean. Next, we extracted the genes that are differentially expressed genes (DEGs) that were extracted using the Rank products method of the Bioconductor software package. These DEGs were sorted into 8 groups using the hclust function according to gene expression patterns. Three of the groups across which the expression levels progressively increased included 100 genes, while 3 groups across which the levels decreased contained 47 genes. Storage proteins, seed-maturation proteins, some protease inhibitors, and the allergen Gly m Bd 28K were classified into the former groups. Lipoxygenase (LOX) family members were present in both the groups, indicating the multi-functionality with different expression patterns.
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. asakura@mail.ecc.u-tokyo.ac.jp
authors.forename: Tomiko
authors.initials: T
authors.lastname: Asakura
authors.forename: Tomoko
authors.initials: T
authors.lastname: Tamura
authors.forename: Kaede
authors.initials: K
authors.lastname: Terauchi
authors.forename: Tomoyo
authors.initials: T
authors.lastname: Narikawa
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Yagasaki
authors.forename: Yoshiro
authors.initials: Y
authors.lastname: Ishimaru
authors.forename: Keiko
authors.initials: K
authors.lastname: Abe
mesh_terms: D016000:Cluster Analysis; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D010940:Plant Proteins / Q000235:genetics*; D018749:RNA, Plant / Q000235:genetics; D012639:Seeds / Q000235:genetics / Q000254:growth & development*; D013025:Glycine max / Q000235:genetics* / Q000254:growth & development; D059467:Transcriptome
chemical_list: D010940:Plant Proteins; D018749:RNA, Plant