title: [Expression of structural and non-structural proteins of severe fever with thrombocytopenia syndrome bunyavirus].
abstract: Severe fever with thrombocytopenia syndrome bunyavirus (SFTSV) is a novel phlebovirus, causing a life-threatening illness associated with the symptoms of severe fever and thrombocytopenia syndrome. The sequence and structure of the genome have already been illustrated in previous study. However, the characteristics and function of the structure and non-structure proteins is still unclear. In this study, we identified the density of the purified SFTSV virions as 1.135 g/mL in sucrose solution. Using RT-PCR method, we amplified the full coding sequence of RNA dependent RNA polymerase(RdRp), glycoprotein precursor (M), glycoprotein n (Gn), glycoprotein c (Gc), nuclear protein (NP) and non structural protein (NSs) of SFTSV (strain HB29). Respectively inserted the target genes into eukaryotic expression vector pcDNA5/FRT or VR1012, the target protein in 293T cell were successfully expressed. By analyzing the SFTSV virions in SDS-PAGE and using recombinant viral proteins with SFTS patients sera in Western blotting and Immunofluorescent assay, the molecule weight of structure and non-structure proteins of SFTSV were defined. The study provides the first step to understand the molecular characteristics of SFTSV.
authors.affiliation: National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Lu
authors.forename: Chuan
authors.initials: C
authors.lastname: Li
authors.forename: Fu-Shun
authors.initials: FS
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.forename: Quan-Fu
authors.initials: QF
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Qin
authors.initials: Q
authors.lastname: Wang
authors.forename: Pei-Hong
authors.initials: PH
authors.lastname: Qiu
authors.forename: Mi-Fang
authors.initials: MF
authors.lastname: Liang
authors.forename: De-Xin
authors.initials: DX
authors.lastname: Li
mesh_terms: D002044:Bunyaviridae Infections / Q000821:virology*; D002461:Cell Line, Transformed; D005334:Fever / Q000821:virology*; D057809:HEK293 Cells; D006801:Humans; D016851:Orthobunyavirus / Q000235:genetics* / Q000378:metabolism; D013921:Thrombocytopenia / Q000821:virology*; D017361:Viral Nonstructural Proteins / Q000096:biosynthesis* / Q000235:genetics; D015678:Viral Structural Proteins / Q000096:biosynthesis* / Q000235:genetics; D014771:Virion / Q000235:genetics* / Q000378:metabolism
chemical_list: D017361:Viral Nonstructural Proteins; D015678:Viral Structural Proteins