title: High-throughput method for the analysis of venlafaxine in pharmaceutical formulations and biological fluids, using a tris(2,2'-bipyridyl) ruthenium(II)-peroxydisulphate chemiluminescence system in a two-chip device.
abstract: A simple, rapid and sensitive chemiluminescent (CL) method for the assay of venlafaxine (VEN) in pharmaceutical formulations and serum samples by a two-chip device is proposed. The method is based on the reaction of this drug with a tris(2,2'-bipyridyl) ruthenium(II)-peroxydisulphate CL system. The optimum chemical conditions for CL emission were investigated. The calibration graph was linear for the concentration range 0.02-8.0 µg/mL. The detection and quantification limits were found to be 0.006 and 0.018 µg/mL, respectively, while the relative standard deviation (RSD) was <2.0%. The present CL procedure was applied to the determination of VEN in pharmaceutical formulations and serum samples; the recovery levels were in the range 96.5-101.2%. The results suggest that the method is unaffected by the presence of common formulation excipients found in these samples.
authors.affiliation: Department of Chemistry, College of Science, Sultan Qaboos University, Al-Khod, Oman. haiderl@squ.edu.om
authors.forename: Haider A J
authors.initials: HA
authors.lastname: Al Lawati
authors.forename: Gouri B
authors.initials: GB
authors.lastname: Varma
authors.forename: Fakhr Eldin O
authors.initials: FE
authors.lastname: Suliman
mesh_terms: D002626:Chemistry, Pharmaceutical; D003511:Cyclohexanols / Q000032:analysis* / Q000097:blood; D057166:High-Throughput Screening Assays / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D056656:Lab-On-A-Chip Devices; D049408:Luminescent Agents / Q000737:chemistry*; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D046208:Microchip Analytical Procedures / Q000379:methods; D004364:Pharmaceutical Preparations / Q000032:analysis*; D012428:Ruthenium / Q000737:chemistry; D000069470:Venlafaxine Hydrochloride
chemical_list: D003511:Cyclohexanols; D049408:Luminescent Agents; D004364:Pharmaceutical Preparations; D000069470:Venlafaxine Hydrochloride; D012428:Ruthenium