title: The importance of being supercoiled: how DNA mechanics regulate dynamic processes.
abstract: Through dynamic changes in structure resulting from DNA-protein interactions and constraints given by the structural features of the double helix, chromatin accommodates and regulates different DNA-dependent processes. All DNA transactions (such as transcription, DNA replication and chromosomal segregation) are necessarily linked to strong changes in the topological state of the double helix known as torsional stress or supercoiling. As virtually all DNA transactions are in turn affected by the torsional state of DNA, these changes have the potential to serve as regulatory signals detected by protein partners. This two-way relationship indicates that DNA dynamics may contribute to the regulation of many events occurring during cell life. In this review we will focus on the role of DNA supercoiling in the cellular processes, with particular emphasis on transcription. Besides giving an overview on the multiplicity of factors involved in the generation and dissipation of DNA torsional stress, we will discuss recent studies which give new insight into the way cells use DNA dynamics to perform functions otherwise not achievable. This article is part of a Special Issue entitled: Chromatin in time and space.
authors.affiliation: Laboratory of Pathology, National Cancer Institute, Bethesda, MD 20892-1500, USA.
authors.forename: Laura
authors.initials: L
authors.lastname: Baranello
authors.forename: David
authors.initials: D
authors.lastname: Levens
authors.forename: Ashutosh
authors.initials: A
authors.lastname: Gupta
authors.forename: Fedor
authors.initials: F
authors.lastname: Kouzine
mesh_terms: D000818:Animals; D002843:Chromatin / Q000235:genetics / Q000378:metabolism; D042002:Chromatin Assembly and Disassembly; D004278:DNA, Superhelical / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000378:metabolism / Q000502:physiology; D005786:Gene Expression Regulation; D006801:Humans; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic
chemical_list: D002843:Chromatin; D004278:DNA, Superhelical; D004268:DNA-Binding Proteins