title: ENU-based phenotype-driven screening.
abstract: Deciphering the contribution of individual genes and in turn pathways to cellular processes can be complicated and is often based on prior knowledge or assumptions of gene function. Phenotype-driven mutagenesis screens based around n-ethyl-n-nitrosurea (ENU) have been successful in a wide range of physiological systems in identifying novel genes that contribute to a given phenotype. Here, we describe methodologies we have employed in analysing cellular phenotypes in pipelines of mutagenised mice. Examples of primary screens to identify outliers, and secondary screens to provide a more detailed characterisation are outlined.
authors.affiliation: Mammalian Genetics Unit, Medical Research Council Harwell, Oxfordshire, UK.
authors.forename: Vera M
authors.initials: VM
authors.lastname: Ripoll
authors.forename: Philip L
authors.initials: PL
authors.lastname: Kong
authors.forename: Paul K
authors.initials: PK
authors.lastname: Potter
mesh_terms: D000818:Animals; D001772:Blood Cell Count / Q000295:instrumentation / Q000379:methods; D001854:Bone Marrow Cells / Q000378:metabolism; D001947:Breeding; D016207:Cytokines / Q000032:analysis / Q000378:metabolism; D005038:Ethylnitrosourea / Q000008:administration & dosage / Q000633:toxicity*; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D007962:Leukocytes / Q000187:drug effects* / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D016296:Mutagenesis; D010641:Phenotype
chemical_list: D016207:Cytokines; D005038:Ethylnitrosourea