title: Association of CTLA-4 gene polymorphisms with sporadic breast cancer risk and clinical features in Han women of northeast China.
abstract: Cytotoxic T lymphocyte antigen-4 (CTLA-4) is an inhibitory molecule that plays a pivotal role in downregulating T-cell mediated immune responses. To determine the role of CTLA-4 in tumor immunity, and to validate previous results as well, we investigated four tag single nucleotide polymorphisms (SNPs) of CTLA-4 in a relatively large Chinese Han cohort from northeastern China. Genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 581 patients and 566 age-matched controls. Our data indicated that compared with the common genotype and allele of each SNP, the -1722 CC genotype and C allele showed an increased risk of breast cancer (P = 0.030, odds ratio (OR) = 1.457, 95% confidence internal (CI) 1.036-2.051; P = 0.024, OR = 1.214, 95% CI 1.026-1.436, respectively). The -1661 GG genotype and G allele were also associated with an increased risk of breast cancer (P = 0.018, OR = 1.396, 95% CI 1.058-1.843; P = 0.013, OR = 1.353, 95% CI 1.066-1.717, respectively). In the haplotype analysis, the CAAA haplotype showed a higher frequency in cases (P = 0.004), and this association remained significant after correcting the P value for multiple testing. Associations were shown between the SNPs of CTLA-4 and lymph node metastasis, estrogen receptor (ER), progesterone receptor (PR) and P53 statuses. These results indicate that some SNPs in the CTLA-4 gene may affect the risk of breast cancer and show that some SNPs are associated with breast cancer characteristics in Han women in northeastern China.
authors.affiliation: Department of Surgery, The Third Affiliated Hospital of Harbin Medical University, Harbin 150081, China. lidalin1975@163.com
authors.forename: Dalin
authors.initials: D
authors.lastname: Li
authors.forename: Qiujin
authors.initials: Q
authors.lastname: Zhang
authors.forename: Fengyan
authors.initials: F
authors.lastname: Xu
authors.forename: Zhenkun
authors.initials: Z
authors.lastname: Fu
authors.forename: Weiguang
authors.initials: W
authors.lastname: Yuan
authors.forename: Dianjun
authors.initials: D
authors.lastname: Li
authors.forename: Da
authors.initials: D
authors.lastname: Pang
mesh_terms: D000328:Adult; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D060908:CTLA-4 Antigen / Q000235:genetics*; D002681:China; D005260:Female; D056726:Genetic Association Studies; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D008198:Lymph Nodes / Q000473:pathology; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism; D011960:Receptors, Estrogen / Q000378:metabolism; D012306:Risk; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D060908:CTLA-4 Antigen; D018719:Erb-b2 Receptor Tyrosine Kinases; D011960:Receptors, Estrogen; D016159:Tumor Suppressor Protein p53; C556706:CTLA4 protein, human; C495901:TP53 protein, human