title: Effect of growth promotants on the occurrence of endogenous and synthetic steroid hormones on feedlot soils and in runoff from beef cattle feeding operations.
abstract: Supplements and growth promotants containing steroid hormones are routinely administered to beef cattle to improve feeding efficiency, reduce behavioral problems, and enhance production. As a result, beef cattle manure will contain both synthetic steroids as well as a range of endogenous steroids including androgens, estrogens, and progestogens. A two-year controlled study was conducted in which beef cattle were administered steroid hormones via subcutaneous implants and feed additives and the occurrence of 16 endogenous and synthetic steroid hormones and metabolites was evaluated in runoff from beef cattle feedlots and in manure and soil collected from feedlot surfaces. Samples were extracted and analyzed using liquid chromatography tandem mass spectrometryfor metabolites of the synthetic androgen trenbolone acetate, 17α-trenbolone, 17β-trenbolone, for the nonsteroidal semisynthetic estrogen agonist, α-zearalanol, and the synthetic progesterone melengesterol acetate, as well as a wide range of endogeneous estrogens, androgens, and fusarium metabolites. Synthetic steroids including trenbolone metabolites and melengestrol acetate were detected in fresh manure and in feedlot surface soils from cattle administered synthetic steroids at concentrations up to 55 ± 22 ng/g dry weight (dw) (17α-trenbolone) and 6.5 ± 0.4 ng/g dw (melengesterol acetate). Melengesterol acetate was detected in 6% of runoff samples from feedlots holding cattle administered synthetic steroids at concentrations ranging up to 115 ng/L. The presence of melengesterol acetate in runoff from beef cattle feeding operations has not been previously reported. Synthetic steroids were not detected in manure or runoff from control cattle. A wide range of endogenous hormones were detected in runoff and feedlot surface soils and manure from cattle given synthetic steroids and from control cattle, with no statistically significant differences in concentration. These results indicate that runoff from confined animal production facilities is of environmental and public health concern regardless of the use of growth promotants.
authors.affiliation: Department of Civil Engineering, University of Nebraska-Lincoln, Peter Kiewit Institute, Omaha, Nebraska 68182-0178, United States. sbartelt2@unl.edu
authors.forename: Shannon L
authors.initials: SL
authors.lastname: Bartelt-Hunt
authors.forename: Daniel D
authors.initials: DD
authors.lastname: Snow
authors.forename: William L
authors.initials: WL
authors.lastname: Kranz
authors.forename: Terry L
authors.initials: TL
authors.lastname: Mader
authors.forename: Charles A
authors.initials: CA
authors.lastname: Shapiro
authors.forename: Simon J van
authors.initials: SJ
authors.lastname: Donk
authors.forename: David P
authors.initials: DP
authors.lastname: Shelton
authors.forename: David D
authors.initials: DD
authors.lastname: Tarkalson
authors.forename: Tian C
authors.initials: TC
authors.lastname: Zhang
mesh_terms: D000383:Agriculture / Q000379:methods*; D000818:Animals; D002417:Cattle; D002853:Chromatography, Liquid; D019587:Dietary Supplements / Q000032:analysis*; D004784:Environmental Monitoring / Q000379:methods / Q000706:statistics & numerical data*; D012739:Gonadal Steroid Hormones / Q000819:agonists / Q000032:analysis*; D006133:Growth Substances / Q000032:analysis; D008372:Manure / Q000032:analysis*; D008552:Melengestrol Acetate / Q000032:analysis; D012989:Soil Pollutants / Q000032:analysis*; D053719:Tandem Mass Spectrometry; D014204:Trenbolone Acetate / Q000032:analysis; D014874:Water Pollutants, Chemical / Q000032:analysis*; D015029:Zeranol / Q000032:analysis
chemical_list: D012739:Gonadal Steroid Hormones; D006133:Growth Substances; D008372:Manure; D012989:Soil Pollutants; D014874:Water Pollutants, Chemical; D008552:Melengestrol Acetate; D015029:Zeranol; D014204:Trenbolone Acetate