title: Interdigitated electrodes based on impedance biosensor for sensing peptide LL-37.
abstract: An antimicrobial peptide, LL-37, is found in an innate defense system of humans. Patients who suffer urinary tract infection (UTI) will generate LL-37 and which is released into urine. LL-37 can be used as an indicator for the diagnosis of UTI. We have designed a biosensor with an interdigitated electrode on a printed-circuit board (PCB). The surface of the electrode was modified with 3-mercaptopropionic acid and immobilized with anti-LL37 antibody to improve the specificity of the biosensor. By de-embedding jig impedance, the impedance associated with the change of LL-37 concentration was calculated. The sensitivity of this biosensor for LL-37 in a urine sample can reach 50 μg/mL.
authors.affiliation: Institute of Electrical Engineering, Tatung University, 40 Chung-Shan N Rd, Sec 3, Taipei 104, Taiwan. smalldemon2001@hotmail.com
authors.forename: Jia-Yi
authors.initials: JY
authors.lastname: Lee
authors.forename: Chung-Yih
authors.initials: CY
authors.lastname: Wang
authors.forename: Chi-Fang
authors.initials: CF
authors.lastname: Huang
authors.forename: An-Ting
authors.initials: AT
authors.lastname: Cheng
mesh_terms: D023181:Antimicrobial Cationic Peptides / Q000652:urine*; D015374:Biosensing Techniques / Q000295:instrumentation*; D003217:Conductometry / Q000295:instrumentation*; D017097:Electric Impedance; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D054804:Cathelicidins
chemical_list: D023181:Antimicrobial Cationic Peptides; D054804:Cathelicidins