title: Flavobacterium rakeshii sp. nov., isolated from marine sediment, and emended description of Flavobacterium beibuense Fu et al. 2011.
abstract: A Gram-negative, non-motile bacterial strain that formed straight rods and straw yellow colonies, designated FCS-5(T), was isolated from a marine sediment from the Arabian Sea. The isolate exhibited most of the phenotypic properties expected for a member of the genus Flavobacterium. The major fatty acids were iso-C(15:0), iso-C(17:0) 3-OH, C(17:1)ω9c and summed feature 3 (comprising iso-C(15:0) 2-OH and/or C(16:1)ω7c). The only isoprenoid quinone was MK-6. The only polyamine was homospermidine and the major polar lipid was phosphatidylethanolamine. The G+C content of the genomic DNA was 32.4 mol%. According to 16S rRNA gene sequence analysis, strain FCS-5(T) belonged to the genus Flavobacterium and exhibited 99.3% 16S rRNA gene sequence similarity with Flavobacterium beibuense F44-8(T) and 90.9-94.6% sequence similarity with other members of the genus Flavobacterium. The results of physiological and biochemical tests allowed the discrimination of the isolate from its phylogenetic relatives. Strain FCS-5(T) is a representative of a novel species of the genus Flavobacterium, for which the name Flavobacterium rakeshii sp. nov. is proposed. The type strain is FCS-5(T) ( = MTCC 10967(T) = JCM 17928(T)). An emended description of F. beibuense is also proposed.
authors.affiliation: Microbial Type Culture Collection & Gene Bank (MTCC), Institute of Microbial Technology (IMTECH), Sector 39-A, Chandigarh, 160 036, India.
authors.forename: Ishwinder
authors.initials: I
authors.lastname: Kaur
authors.affiliation: Microbial Type Culture Collection & Gene Bank (MTCC), Institute of Microbial Technology (IMTECH), Sector 39-A, Chandigarh, 160 036, India.
authors.forename: Chandandeep
authors.initials: C
authors.lastname: Kaur
authors.affiliation: Microbial Type Culture Collection & Gene Bank (MTCC), Institute of Microbial Technology (IMTECH), Sector 39-A, Chandigarh, 160 036, India.
authors.forename: Fazlurrahman
authors.initials: F
authors.lastname: Khan
authors.affiliation: Microbial Type Culture Collection & Gene Bank (MTCC), Institute of Microbial Technology (IMTECH), Sector 39-A, Chandigarh, 160 036, India.
authors.forename: Shanmugam
authors.initials: S
authors.lastname: Mayilraj
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005417:Flavobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D019015:Geologic Sediments / Q000382:microbiology*; D007194:India; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010714:Phosphatidylethanolamines / Q000032:analysis; D010802:Phylogeny; D011073:Polyamines / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D011073:Polyamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C042455:menaquinone 6