title: Antibodies to a novel leptospiral protein, LruC, in the eye fluids and sera of horses with Leptospira-associated uveitis.
abstract: Screening of an expression library of Leptospira interrogans with eye fluids from uveitic horses resulted in identification of a novel protein, LruC. LruC is located in the inner leaflet of the leptospiral outer membrane, and an lruC gene was detected in all tested pathogenic L. interrogans strains. LruC-specific antibody levels were significantly higher in eye fluids and sera of uveitic horses than healthy horses. These findings suggest that LruC may play a role in equine leptospiral uveitis.
authors.affiliation: M. H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, Kentucky, USA. ashutosh.verma@uky.edu
authors.forename: Ashutosh
authors.initials: A
authors.lastname: Verma
authors.forename: James
authors.initials: J
authors.lastname: Matsunaga
authors.forename: Sergey
authors.initials: S
authors.lastname: Artiushin
authors.forename: Marija
authors.initials: M
authors.lastname: Pinne
authors.forename: Dirk J
authors.initials: DJ
authors.lastname: Houwers
authors.forename: David A
authors.initials: DA
authors.lastname: Haake
authors.forename: Brian
authors.initials: B
authors.lastname: Stevenson
authors.forename: John F
authors.initials: JF
authors.lastname: Timoney
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis* / Q000097:blood; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology; D001826:Body Fluids / Q000276:immunology*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005123:Eye / Q000276:immunology*; D006734:Horse Diseases / Q000276:immunology* / Q000473:pathology; D006736:Horses; D007919:Leptospira / Q000276:immunology* / Q000472:pathogenicity; D007922:Leptospirosis / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D014605:Uveitis / Q000276:immunology / Q000473:pathology / Q000662:veterinary*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial