title: Fast and simultaneous determination of urinary 8-hydroxy-2'-deoxyguanosine and ten monohydroxylated polycyclic aromatic hydrocarbons by liquid chromatography/tandem mass spectrometry.
abstract: 8-Hydroxy-2'-deoxyguanosine (8-OHdG), a biomarker of oxidative DNA damage, has been extensively studied to assess human exposure to carcinogenic compounds. Previous studies have associated levels of human urinary hydroxylated polycyclic aromatic hydrocarbons (OH-PAHs) with those of 8-OHdG. However, measurements of OH-PAHs and 8-OHdG in urine are often conducted with two different analytical methods, which is both costly and time-consuming. In this study, a novel method is described to quickly and simultaneously quantify ten urinary OH-PAHs and 8-OHdG through high pressure liquid chromatography/tandem mass spectrometry (HPLC/MS/MS). Urine samples undergo solid phase extraction and concentration and then are analyzed by an optimized HPLC/MS/MS method operated in the negative electrospray ionization (ESI) and multiple reaction monitoring (MRM) mode. Deuterated, (15)N and (13)C- labeled analogues are used as internal standards. Simultaneous analysis of urinary 8-OHdG and OH-PAHs are completed within 16 min. Calibration curves of all target analytes show favorable linearity within the concentration range of 0.3-10.0 μg/L for 8-OHdG and 0.05-15 μg/L for different OH-PAHs. The method detection limits (MDLs) in pooled urine range from 0.023 μg/L to 0.625 μg/L. The method shows satisfactory accuracy and precision when we analyzed varied levels spiked in pooled urine. Recoveries for 8 of the 10 OH-PAHs were in the range of 100 ± 15% with a variation coefficient of less than 20%. Thirty-four real urine samples were analyzed for all target analytes. Except 3-OHF, most compounds could be quantified.
authors.affiliation: Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, College of Life Science, South China Normal University , Guangzhou, China 510631.
authors.forename: Ruifang
authors.initials: R
authors.lastname: Fan
authors.forename: Dongli
authors.initials: D
authors.lastname: Wang
authors.forename: Robert
authors.initials: R
authors.lastname: Ramage
authors.forename: Jianwen
authors.initials: J
authors.lastname: She
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D015415:Biomarkers / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D004785:Environmental Pollutants / Q000737:chemistry / Q000652:urine*; D006801:Humans; D006900:Hydroxylation; D011084:Polycyclic Aromatic Hydrocarbons / Q000737:chemistry / Q000652:urine*; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D015415:Biomarkers; D004785:Environmental Pollutants; D011084:Polycyclic Aromatic Hydrocarbons; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine