title: Genetic analysis of hepatitis A virus strains that induced epidemics in Korea during 2007-2009.
abstract: Hepatitis A virus is one of the most prominent causes of fecally transmitted acute hepatitis worldwide. In order to characterize the viral agents causing an outbreak in Korea (comprising North and South Korea) from June 2007 to May 2009, we collected specimens and performed genotyping of the VP1/P2A and VP3/VP1 regions of hepatitis A virus. We then used a multiple-alignment algorithm to compare the nucleotide sequences of the 2 regions with those of reference strains. Hepatitis A virus antibodies were detected in 64 patients from 5 reported outbreaks (North Korea, June 2007 [n = 11]; Jeonnam, April 2008 [n = 15]; Daegu, May 2008 [n = 13]; Seoul, May 2009 [n = 22]; and Incheon, May 2009 [n = 3]). We found 100% homology between strains isolated from the Kaesong Industrial Region and Jeonnam. While those strains were classified as genotype IA strains, strains from Seoul and Incheon were identified as genotype IIIA strains and showed 98.9 to 100% homology. Genotype IIIA was also dominant in Daegu, where strains were 95.7 to 100% homologous. All hepatitis A virus strains isolated from the Kaesong Industrial Region, Jeonnam, Seoul, and Incheon belonged to a single cluster. However, strains from Daegu could be classified into 2 clusters, suggesting that the outbreak had multiple sources. This study indicates that hepatitis A virus strains of 2 different genotypes are currently cocirculating in Korea. Moreover, it documents an increasing prevalence of genotype IIIA strains in the country.
authors.affiliation: Division of Enteric and Hepatitis Viruses, Center for Infectious Diseases, National Institute of Health, Korea Centers for Disease Control and Prevention, Chungbuk, Republic of Korea.
authors.forename: Hyeokjin
authors.initials: H
authors.lastname: Lee
authors.forename: Hyesook
authors.initials: H
authors.lastname: Jeong
authors.forename: Heasun
authors.initials: H
authors.lastname: Yun
authors.forename: Kisang
authors.initials: K
authors.lastname: Kim
authors.forename: Jong-Hyun
authors.initials: JH
authors.lastname: Kim
authors.forename: Jai Myung
authors.initials: JM
authors.lastname: Yang
authors.forename: Doo-Sung
authors.initials: DS
authors.lastname: Cheon
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D058872:Epidemics; D005260:Female; D005838:Genotype; D006506:Hepatitis A / Q000453:epidemiology / Q000821:virology*; D017957:Hepatitis A Virus, Human / Q000235:genetics*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D058889:Molecular Typing; D010802:Phylogeny; D056910:Republic of Korea / Q000453:epidemiology; D017423:Sequence Analysis, RNA; D015678:Viral Structural Proteins / Q000235:genetics*
chemical_list: D015678:Viral Structural Proteins