title: Role of estrogen receptor alpha on vaginal epithelialization of patients with Mayer-Rokitansky-Kuster-Hauser syndrome submitted to neovaginoplasty using oxidized regenerated cellulose.
abstract: OBJECTIVE
  The purpose of this study was to evaluate the expression of estrogen receptor alpha (ERα) on the neovaginal tissue of patients with Mayer-Rokitansky-Kuster-Hauser (MRKH) syndrome submitted to modified Abbé-McIndoe neovaginoplasty using oxidized regenerated cellulose.
  
  
  METHODS
  The current study involved eight subjects with MRKH syndrome. Serial samples of the neovagina were obtained after the surgery and immunohistochemical detection of ERα was performed using antibody NCL-L-ER-6F11.
  
  
  RESULTS
  The operation was performed successfully in all subjects without complications. A total of 22 samples of neovaginal tissue were analyzed. The expression of ERα was detected only 6 months after the surgery, when the neovagina acquired characteristics of normal vagina.
  
  
  CONCLUSIONS
  In our study, the expression of ERα occurred when complete epithelialization of vaginal tissue was observed. Other mechanisms may be involved in the formation of vaginal epithelium in patients with MRKH syndrome.
authors.affiliation: marciomk@hotmail.com
authors.forename: Marcio Masashi
authors.initials: MM
authors.lastname: Kajikawa
authors.forename: Zsuzsanna Ilona Katalin
authors.initials: ZI
authors.lastname: Jármy-Di Bella
authors.forename: Gustavo Rubino de Azevedo
authors.initials: GR
authors.lastname: Focchi
authors.forename: Juliane
authors.initials: J
authors.lastname: Dornelas
authors.forename: Manoel João Batista Castello
authors.initials: MJ
authors.lastname: Girão
authors.forename: Marair Gracio Ferreira
authors.initials: MG
authors.lastname: Sartori
mesh_terms: D058489:46, XX Disorders of Sex Development / Q000601:surgery*; D000015:Abnormalities, Multiple / Q000601:surgery*; D000293:Adolescent; D000328:Adult; D001706:Biopsy; D002483:Cellulose, Oxidized; D000013:Congenital Abnormalities; D004848:Epithelium / Q000473:pathology / Q000502:physiology; D047628:Estrogen Receptor alpha / Q000502:physiology*; D004967:Estrogens; D005260:Female; D013509:Gynecologic Surgical Procedures / Q000379:methods*; D006801:Humans; D007668:Kidney / Q000002:abnormalities; D009095:Mullerian Ducts / Q000002:abnormalities; D019651:Plastic Surgery Procedures / Q000379:methods*; D012189:Retrospective Studies; D019170:Somites / Q000002:abnormalities; D013131:Spine / Q000002:abnormalities; D016896:Treatment Outcome; D014599:Uterus / Q000002:abnormalities / Q000601:surgery; D014621:Vagina / Q000002:abnormalities / Q000473:pathology / Q000502:physiology* / Q000601:surgery*; D055815:Young Adult
chemical_list: D002483:Cellulose, Oxidized; D047628:Estrogen Receptor alpha; D004967:Estrogens