title: Thermosensitive eyedrops containing platelet lysate for the treatment of corneal ulcers.
abstract: Corneal lesions cause significant pain and visual impairment and, in many cases, are unresponsive to conventional treatments. Platelet lysate (PL) is an haemoderivative rich in growth factors (GFs) that are released by platelets after freeze-thawing destruction of platelet rich plasma (PRP). The aim of the present work was to develop thermosensitive and mucoadhesive eyedrops to maintain and prolong the contact of platelet lysate (PL) with cornea ulcers. A sterile vehicle based on chondroitin sulphate sodium (CS) and hydroxypropylmethyl cellulose (HPMC) was developed. An extemporaneous loading of the vehicle with PL was performed and the obtained formulation was able to quickly thermogelify at about 32 °C and was characterized by good mucoadhesive properties. ELISA evidenced that the growth factor PDGF AB was compatible with the vehicle and stable in the formulation up to 15 days of storage at 2-8 °C. In vitro wound healing and proliferation test (performed using rabbit corneal epithelial cells (RCE)) showed that the formulation enhanced cell growth and put in evidence a synergistic effect of CS and PL in stimulating cell proliferation. The overall results indicate that PL loaded in thermosensitive and mucoadhesive eyedrops can be profitably employed to treat corneal lesions.
authors.affiliation: Department of Drug Sciences, University of Pavia, Pavia 27100, Italy.
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Sandri
authors.affiliation: Department of Drug Sciences, University of Pavia, Pavia 27100, Italy.
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Bonferoni
authors.affiliation: Department of Drug Sciences, University of Pavia, Pavia 27100, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Rossi
authors.affiliation: Department of Drug Sciences, University of Pavia, Pavia 27100, Italy.
authors.forename: Franca
authors.initials: F
authors.lastname: Ferrari
authors.affiliation: Department of Drug Sciences, University of Pavia, Pavia 27100, Italy.
authors.forename: Michela
authors.initials: M
authors.lastname: Mori
authors.affiliation: Immunohaematology and Transfusion Service, Apheresis and Cell Therapy Unit, IRRCS Policlinico San Matteo, Pavia 27100, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Del Fante
authors.affiliation: Immunohaematology and Transfusion Service, Apheresis and Cell Therapy Unit, IRRCS Policlinico San Matteo, Pavia 27100, Italy.
authors.forename: Cesare
authors.initials: C
authors.lastname: Perotti
authors.affiliation: Department of Drug Sciences, University of Pavia, Pavia 27100, Italy. Electronic address: carla.caramella@unipv.it.
authors.forename: Carla
authors.initials: C
authors.lastname: Caramella
mesh_terms: D000268:Adhesiveness; D000818:Animals; D001688:Biological Products / Q000737:chemistry / Q000494:pharmacology*; D001792:Blood Platelets / Q000737:chemistry; D002457:Cell Extracts; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002626:Chemistry, Pharmaceutical; D002809:Chondroitin Sulfates / Q000737:chemistry; D003320:Corneal Ulcer / Q000188:drug therapy* / Q000473:pathology; D004337:Drug Carriers; D004797:Enzyme-Linked Immunosorbent Assay; D019573:Epithelium, Corneal / Q000187:drug effects* / Q000473:pathology; D005782:Gels; D006801:Humans; D065347:Hypromellose Derivatives; D008747:Methylcellulose / Q000031:analogs & derivatives / Q000737:chemistry; D009883:Ophthalmic Solutions; D010982:Platelet-Derived Growth Factor / Q000032:analysis; D011817:Rabbits; D012212:Rheology; D013696:Temperature; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D001688:Biological Products; D002457:Cell Extracts; D004337:Drug Carriers; D005782:Gels; D009883:Ophthalmic Solutions; D010982:Platelet-Derived Growth Factor; C095477:platelet-derived growth factor AB; D065347:Hypromellose Derivatives; D008747:Methylcellulose; D002809:Chondroitin Sulfates