title: Supercapacitors based on c-type cytochromes using conductive nanostructured networks of living bacteria.
abstract: Supercapacitors have attracted interest in energy storage because they have the potential to complement or replace batteries. Here, we report that c-type cytochromes, naturally immersed in a living, electrically conductive microbial biofilm, greatly enhance the device capacitance by over two orders of magnitude. We employ genetic engineering, protein unfolding and Nernstian modeling for in vivo demonstration of charge storage capacity of c-type cytochromes and perform electrochemical impedance spectroscopy, cyclic voltammetry and charge-discharge cycling to confirm the pseudocapacitive, redox nature of biofilm capacitance. The biofilms also show low self-discharge and good charge/discharge reversibility. The superior electrochemical performance of the biofilm is related to its high abundance of cytochromes, providing large electron storage capacity, its nanostructured network with metallic-like conductivity, and its porous architecture with hydrous nature, offering prospects for future low cost and environmentally sustainable energy storage devices.
authors.affiliation: Department of Physics, University of Massachusetts, Amherst, Amherst, MA 01003, USA. nikhil@physics.umass.edu
authors.forename: Nikhil S
authors.initials: NS
authors.lastname: Malvankar
authors.forename: Tünde
authors.initials: T
authors.lastname: Mester
authors.forename: Mark T
authors.initials: MT
authors.lastname: Tuominen
authors.forename: Derek R
authors.initials: DR
authors.lastname: Lovley
mesh_terms: D001419:Bacteria / Q000737:chemistry* / Q000378:metabolism; D018441:Biofilms; D003574:Cytochrome c Group / Q000737:chemistry* / Q000378:metabolism; D058266:Dielectric Spectroscopy; D025802:Electric Capacitance; D004566:Electrodes; D045302:Geobacter / Q000502:physiology; D049329:Nanostructures / Q000737:chemistry*; D010084:Oxidation-Reduction
chemical_list: D003574:Cytochrome c Group