title: Aquaporin-4 deficiency attenuates acute lesions but aggravates delayed lesions and microgliosis after cryoinjury to mouse brain.
abstract: OBJECTIVE
  To determine whether aquaporin-4 (AQP4) regulates acute lesions, delayed lesions, and the associated microglial activation after cryoinjury to the brain.
  
  
  METHODS
  Brain cryoinjury was applied to AQP4 knockout (KO) and wild-type mice. At 24 h and on days 7 and 14 after cryoinjury, lesion volume, neuronal loss, and densities of microglia and astrocytes were determined, and their changes were compared between AQP4 KO and wild-type mice.
  
  
  RESULTS
  Lesion volume and neuronal loss in AQP4 KO mice were milder at 24 h following cryoinjury, but worsened on days 7 and 14, compared to those in wild-type mice. Besides, microglial density increased more, and astrocyte proliferation and glial scar formation were attenuated on days 7 and 14 in AQP4 KO mice.
  
  
  CONCLUSIONS
  AQP4 deficiency ameliorates acute lesions, but worsens delayed lesions, perhaps due to the microgliosis in the late phase.
authors.affiliation: Department of Pharmacology and Institute of Neurobiology, School of Medicine, Zhejiang University, Hangzhou, China.
authors.forename: Wen-Zhen
authors.initials: WZ
authors.lastname: Shi
authors.forename: Chun-Zhen
authors.initials: CZ
authors.lastname: Zhao
authors.forename: Bing
authors.initials: B
authors.lastname: Zhao
authors.forename: Xiao-Liang
authors.initials: XL
authors.lastname: Zheng
authors.forename: San-Hua
authors.initials: SH
authors.lastname: Fang
authors.forename: Yun-Bi
authors.initials: YB
authors.lastname: Lu
authors.forename: Wei-Ping
authors.initials: WP
authors.lastname: Zhang
authors.forename: Zhong
authors.initials: Z
authors.lastname: Chen
authors.forename: Er-Qing
authors.initials: EQ
authors.lastname: Wei
mesh_terms: D000818:Animals; D051401:Aquaporin 4 / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D001253:Astrocytes / Q000378:metabolism; D001921:Brain / Q000378:metabolism; D001930:Brain Injuries / Q000473:pathology*; D004195:Disease Models, Animal; D005615:Freezing; D005911:Gliosis / Q000473:pathology*; D051379:Mice; D018345:Mice, Knockout; D017628:Microglia / Q000378:metabolism / Q000473:pathology*
chemical_list: D051401:Aquaporin 4