title: A simple, semiselective medium for anaerobic isolation of anginosus group streptococci from patients with chronic lung disease.
abstract: The anaerobic isolation of anginosus group streptococci (AGS) from respiratory specimens containing diverse microbiota using a semiselective blood agar medium incorporating nalidixic acid and sulfamethazine (NAS) is described. AGS were detected in 60% of tested sputa from patients with cystic fibrosis, chronic obstructive pulmonary disease, and bronchiectasis. This demonstrates NAS to be a diagnostic tool for detecting AGS within the complex microbial communities associated with chronic lung disorders.
authors.affiliation: Centre for Immunology and Infectious Disease, Blizard Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, UK.
authors.forename: Richard D
authors.initials: RD
authors.lastname: Waite
authors.forename: David W
authors.initials: DW
authors.lastname: Wareham
authors.forename: Samuel
authors.initials: S
authors.lastname: Gardiner
authors.forename: Robert A
authors.initials: RA
authors.lastname: Whiley
mesh_terms: D002908:Chronic Disease; D003470:Culture Media; D046508:Culture Techniques; D006801:Humans; D008171:Lung Diseases / Q000150:complications*; D009268:Nalidixic Acid / Q000737:chemistry; D015995:Prevalence; D012141:Respiratory Tract Infections / Q000453:epidemiology / Q000382:microbiology*; D013183:Sputum / Q000382:microbiology; D013290:Streptococcal Infections / Q000453:epidemiology / Q000382:microbiology*; D034366:Streptococcus anginosus / Q000254:growth & development*; D013418:Sulfamethazine / Q000737:chemistry
chemical_list: D003470:Culture Media; D009268:Nalidixic Acid; D013418:Sulfamethazine