title: Evaluation of novel urinary renal biomarkers with a cisplatin model of kidney injury: effects of collection period.
abstract: A number of novel urinary biomarkers have been identified and partially qualified for use as markers for renal injury in rats. To date, all evaluation studies have been made using 18 to 24 hour collection periods. However, shorter, more welfare friendly, urine collection periods are also used in industry. In this article, we quantify urinary biomarker concentration in serial paired sequential short and long urine collections from male rats administered varying concentrations of cisplatin. We calculate the rate of biomarker excretion in normal animals for both collection periods and the bias and correlation in urinary biomarker concentration between collection periods in dosed and control animals, and we estimate the level of agreement in biomarker concentration between both collection periods. We conclude that although there are minor differences in the concentration of some urinary biomarkers that are dependent upon the time and duration of collection, shorter collection protocols do not influence subsequent interpretation of normalized urinary biomarker data for most biomarkers.
authors.affiliation: Safety Assessment, AstraZeneca, Alderley Park, Macclesfield, Cheshire, UK. mark.pinches@astrazeneca.com
authors.forename: Mark D
authors.initials: MD
authors.lastname: Pinches
authors.forename: Catherine J
authors.initials: CJ
authors.lastname: Betts
authors.forename: Susan J
authors.initials: SJ
authors.lastname: Bickerton
authors.forename: Laura
authors.initials: L
authors.lastname: Beattie
authors.forename: Lisa D
authors.initials: LD
authors.lastname: Burdett
authors.forename: Helen T
authors.initials: HT
authors.lastname: Thomas
authors.forename: Nicola A
authors.initials: NA
authors.lastname: Derbyshire
authors.forename: Michele
authors.initials: M
authors.lastname: Moores
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism / Q000652:urine; D002945:Cisplatin / Q000633:toxicity*; D004195:Disease Models, Animal; D005260:Female; D006651:Histocytochemistry; D007674:Kidney Diseases / Q000139:chemically induced* / Q000378:metabolism / Q000652:urine*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D012107:Research Design
chemical_list: D015415:Biomarkers; D002945:Cisplatin