title: CREPT accelerates tumorigenesis by regulating the transcription of cell-cycle-related genes.
abstract: Tumorigenesis is caused by an uncontrolled cell cycle and the altered expression of many genes. Here, we report a gene CREPT that is preferentially expressed in diverse human tumors. Overexpression of CREPT accelerates tumor growth, whereas depletion of CREPT demonstrates a reversed effect. CREPT regulates cyclin D1 expression by binding to its promoter, enhancing its transcription both in vivo and in vitro, and interacting with RNA polymerase II (RNAPII). Interestingly, CREPT promotes the formation of a chromatin loop and prevents RNAPII from reading through the 3' end termination site of the gene. Our findings reveal a mechanism where CREPT increases cyclin D1 transcription during tumorigenesis, through enhancing the recruitment of RNAPII to the promoter region, possibly, as well as chromatin looping.
authors.affiliation: State Key Laboratory of Biomembrane and Membrane Biotechnology, School of Medicine, Tsinghua University, Beijing, China.
authors.forename: Dongdong
authors.initials: D
authors.lastname: Lu
authors.forename: Yinyuan
authors.initials: Y
authors.lastname: Wu
authors.forename: Yinyin
authors.initials: Y
authors.lastname: Wang
authors.forename: Fangli
authors.initials: F
authors.lastname: Ren
authors.forename: Dianjun
authors.initials: D
authors.lastname: Wang
authors.forename: Fuqin
authors.initials: F
authors.lastname: Su
authors.forename: Yanquan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xi
authors.initials: X
authors.lastname: Yang
authors.forename: Guihua
authors.initials: G
authors.lastname: Jin
authors.forename: Xinbao
authors.initials: X
authors.lastname: Hao
authors.forename: Dacheng
authors.initials: D
authors.lastname: He
authors.forename: Yonggong
authors.initials: Y
authors.lastname: Zhai
authors.forename: David M
authors.initials: DM
authors.lastname: Irwin
authors.forename: Jim
authors.initials: J
authors.lastname: Hu
authors.forename: Joseph J Y
authors.initials: JJ
authors.lastname: Sung
authors.forename: Jun
authors.initials: J
authors.lastname: Yu
authors.forename: Baoqing
authors.initials: B
authors.lastname: Jia
authors.forename: Zhijie
authors.initials: Z
authors.lastname: Chang
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000235:genetics; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000235:genetics* / Q000378:metabolism; D047108:Embryonic Development / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D019940:Genes, bcl-1 / Q000235:genetics; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism; D025941:Protein Interaction Mapping; D012319:RNA Polymerase II / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: C571224:CREPT protein, mouse; D018797:Cell Cycle Proteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger; C571225:RPRD1B protein, human; D012319:RNA Polymerase II