title: Antibacterial activity of Thymus maroccanus and Thymus broussonetii essential oils against nosocomial infection - bacteria and their synergistic potential with antibiotics.
abstract: The aim of this study was to evaluate the antibacterial effect of the association between conventional antibiotics and essential oils (EOs) of endemic Moroccan thyme species, Thymus maroccanus and T. broussonetii, on antibiotic-resistant bacteria involved in nosocomial infections. Synergistic interactions between antibiotics (ciprofloxacin, gentamicin, pristinamycin, and cefixime) and EOs, and between T. maroccanus and T. Broussonetii EOs were determined by the checkerboard test. Serial dilutions of two antimicrobial agents were mixed together so that each row (and column) contained a fixed amount of the first agent and increasing amounts of the second one. The results indicate that the oils had a high inhibitory activity against tested bacteria, except for Pseudomonas aeruginosa. In parallel with the increase of cellular killing, the release of 260nm-absorbing materials from bacterial cells, treated with EOs, increased in response to oil concentration. Out of 80 combinations tested between EOs and antibiotics, 71% showed total synergism, 20% had partial synergistic interaction and 9% showed no effect. Combination with carvacrol, the major constituent of T. maroccanus and T. broussonetii, showed also an interesting synergistic effect in combination with ciprofloxacin. The effect on Gram-positive bacteria was more important than on Gram-negative bacteria. These findings are very promising since the use of these combinations for nosocomial infections treatment is likely to reduce the minimum effective dose of the antibiotics, thus minimizing their possible toxic side effects and treatment cost. However, further investigations are needed to assess the potential for therapeutic application.
authors.affiliation: Laboratoire de Biologie et Biotechnologie des Microorganismes, Pôle d'Excellence Régionale AUF, Faculté des Sciences Semlalia, Université Cadi Ayyad, B.P. 2390, 40000 Marrakech, Morocco.
authors.forename: Mariam
authors.initials: M
authors.lastname: Fadli
authors.forename: Asmaa
authors.initials: A
authors.lastname: Saad
authors.forename: Sami
authors.initials: S
authors.lastname: Sayadi
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Chevalier
authors.forename: Nour-Eddine
authors.initials: NE
authors.lastname: Mezrioui
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Pagès
authors.forename: Lahcen
authors.initials: L
authors.lastname: Hassani
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001424:Bacterial Infections / Q000188:drug therapy / Q000382:microbiology; D002939:Ciprofloxacin / Q000494:pharmacology; D003428:Cross Infection / Q000188:drug therapy / Q000382:microbiology; D000081007:Cymenes; D004357:Drug Synergism; D006090:Gram-Negative Bacteria / Q000187:drug effects* / Q000254:growth & development; D006094:Gram-Positive Bacteria / Q000187:drug effects* / Q000254:growth & development; D006801:Humans; D008826:Microbial Sensitivity Tests; D039821:Monoterpenes / Q000494:pharmacology; D009822:Oils, Volatile / Q000737:chemistry / Q000494:pharmacology*; D035261:Plant Components, Aerial / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000254:growth & development; D046930:Thymus Plant / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D000081007:Cymenes; D039821:Monoterpenes; D009822:Oils, Volatile; D010938:Plant Oils; D002939:Ciprofloxacin; C073316:carvacrol