title: cDNA-AFLP analysis of salt-inducible genes expression in Chrysanthemum lavandulifolium under salt treatment.
abstract: Chrysanthemum lavandulifolium (Fisch. ex Trautv.) Makino is a halophyte species that belongs to the Asteraceae family, and the genus Chrysanthemum. It is one of the ancestors of C.×morifolium Ramatella. Understanding the tolerance mechanism associated with salt stress in C. lavandulifolium could provide important information for explaining the salt tolerance of higher plants and could also help enhancing breeding programs of cultivated Chrysanthemum. In this study, cDNA amplified fragment length polymorphism (cDNA-AFLP) was used to detect differential gene expression in leaves of C. lavandulifolium in response to NaCl treatment. The determination of membrane permeablility, peroxidase activity (POD), malon-dialdehyde (MDA), as well as proline and leaf chlorophyll contents under different NaCl concentrations showed that a 200 mM NaCl treatment was an optimal condition for the cDNA-AFLP experiment. Using this concentration during different times (0, 3 h, 12 h, 24 h and 48 h), we obtained 1930 cDNA fragments using 64 primers. After sequencing 234 randomly chosen cDNA clones and BLASTx analyzing, we got 129 expressed sequence tags (ESTs) which had no significant homology with other sequences, 85 ESTs were homologous to genes with known functions, whereas the rest of ESTs showed homology to unclassified or putative proteins. 25 ESTs that were similar to known functional genes involved in several abiotic and biotic stresses were confirmed by semi-quantitative RT-PCR and qRT-PCR. The expression patterns of these salt-responsive genes not only responded to salt stress but also to plant hormones, such as abscisic acid (ABA), and to other abiotic stresses such as drought and cold. These results indicate an extensive cross-talk among several stresses. Our results provide interesting information for further understanding the molecular mechanisms of salt tolerance in C. lavandulifolium.
authors.affiliation: College of Landscape Architecture, Beijing Forestry University, Beijing 100038, China.
authors.forename: Huang
authors.initials: H
authors.lastname: He
authors.forename: Niu
authors.initials: N
authors.lastname: Yajing
authors.forename: Cao
authors.initials: C
authors.lastname: Huawen
authors.forename: Tang
authors.initials: T
authors.lastname: Xingjiao
authors.forename: Xia
authors.initials: X
authors.lastname: Xinli
authors.forename: Yin
authors.initials: Y
authors.lastname: Weilun
authors.forename: Dai
authors.initials: D
authors.lastname: Silan
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D054458:Amplified Fragment Length Polymorphism Analysis / Q000379:methods*; D002463:Cell Membrane Permeability / Q000187:drug effects; D002734:Chlorophyll / Q000378:metabolism; D031202:Chrysanthemum / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000502:physiology; D003080:Cold Temperature; D018076:DNA, Complementary / Q000235:genetics; D055864:Droughts; D020224:Expressed Sequence Tags; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D017343:Genes, Plant / Q000235:genetics; D008315:Malondialdehyde / Q000378:metabolism; D009195:Peroxidase / Q000187:drug effects / Q000378:metabolism; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism / Q000502:physiology; D055049:Salt Tolerance / Q000235:genetics*; D055051:Salt-Tolerant Plants; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000494:pharmacology*; D013315:Stress, Psychological / Q000235:genetics*; D013997:Time Factors
chemical_list: D018076:DNA, Complementary; D010937:Plant Growth Regulators; D002734:Chlorophyll; D012965:Sodium Chloride; D008315:Malondialdehyde; D000040:Abscisic Acid; D009195:Peroxidase