title: The effects of different endocrine disruptors defining compound-specific alterations of gene expression profiles in the developing testis.
abstract: Environmental contaminants considered endocrine disruptors have been shown to affect testis development and function but the mechanisms of action are not clear. We now have analyzed the effects on the transcriptome in testes of mice exposed to mono-(2-ethylhexyl)-phthalate (9.2; 46.3 or 92.7 mg/kg/d), zearalenone (1.3; 3.9 or 6.6 mg/kg/d), lindane (16.6; 32.2 or 64.4 mg/kg/d), bisphenol-A (0.16; 16 or 64 mg/kg/d) or 17β-estradiol (0.006; 0.012 or 0.048 mg/kg/d). The compounds were orally administered in the drinking water during distinct developmental periods: (A) mothers were exposed only during the two weeks before mating; (B) the exposure was continued during pregnancy until birth or (C) exposure was continued for a further four weeks after birth. Testes were studied at four weeks of age. Mono-(2-ethylhexyl)-phthalate and zearalenone, both produced specific alterations of gene signatures. Interestingly, this was irrespective of the concentration of the toxicant or the developmental period during which exposure occurred.
authors.affiliation: Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Ramiro de Maeztu 9, 28040 Madrid, Spain.
authors.forename: Pedro P
authors.initials: PP
authors.lastname: López-Casas
authors.forename: Sefika C
authors.initials: SC
authors.lastname: Mizrak
authors.forename: Luis A
authors.initials: LA
authors.lastname: López-Fernández
authors.forename: María
authors.initials: M
authors.lastname: Paz
authors.forename: Dirk G
authors.initials: DG
authors.lastname: de Rooij
authors.forename: Jesús
authors.initials: J
authors.lastname: del Mazo
mesh_terms: D000284:Administration, Oral; D000367:Age Factors; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D016000:Cluster Analysis; D052244:Endocrine Disruptors / Q000008:administration & dosage / Q000633:toxicity*; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D053263:Gene Regulatory Networks / Q000187:drug effects; D008297:Male; D018811:Maternal Exposure; D008540:Meiosis / Q000187:drug effects; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D009929:Organ Size / Q000187:drug effects; D011247:Pregnancy; D013087:Spermatids / Q000187:drug effects / Q000473:pathology; D013737:Testis / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism / Q000473:pathology; D018675:Toxicity Tests
chemical_list: D052244:Endocrine Disruptors