title: The role of deimination in ATP5b mRNA transport in a transgenic mouse model of multiple sclerosis.
abstract: Deimination refers to conversion of protein-bound arginine into citrulline. An mRNA carrier, RNA binding export factor (REF), present on mitochondria undergoes loss of deimination with impaired ATP5b mRNA transport in ND4 mice (model of multiple sclerosis) compared with the controls. We present evidence of (1) reduced ATP5b mRNA binding strength of non-deiminated REF compared with deiminated REF, (2) impaired ATP5b mRNA transport in ND4 mice and (3) reduced mitochondrial ATP synthase activity on inhibition of deimination in PC12 cells. Impaired deimination of REF and defect in mitochondrial mRNA transport are critical factors in mitochondrial dysfunction in ND4 mice.
authors.affiliation: Bascom Palmer Eye Institute, University of Miami, Miami, Florida 33136, USA.
authors.forename: Di
authors.initials: D
authors.lastname: Ding
authors.forename: Mabel
authors.initials: M
authors.lastname: Enriquez-Algeciras
authors.forename: Kunjan R
authors.initials: KR
authors.lastname: Dave
authors.forename: Miguel
authors.initials: M
authors.lastname: Perez-Pinzon
authors.forename: Sanjoy K
authors.initials: SK
authors.lastname: Bhattacharya
mesh_terms: D025181:ATP Synthetase Complexes / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D002460:Cell Line; D002956:Citrulline / Q000032:analysis; D004195:Disease Models, Animal; D004789:Enzyme Activation; D015870:Gene Expression; D051379:Mice; D008822:Mice, Transgenic; D008928:Mitochondria / Q000378:metabolism; D025261:Mitochondrial Proton-Translocating ATPases / Q000235:genetics* / Q000378:metabolism*; D009103:Multiple Sclerosis / Q000235:genetics* / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism; D011485:Protein Binding; D021381:Protein Transport; D034443:RNA Transport; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism; D051381:Rats; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D009687:Nuclear Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C486334:Refbp1 protein, mouse; D014157:Transcription Factors; D002956:Citrulline; D000255:Adenosine Triphosphate; D025181:ATP Synthetase Complexes; C530489:ATP5b protein, mouse; D025261:Mitochondrial Proton-Translocating ATPases