title: The relationship between follicle development and progesterone receptor membrane component-1 expression in women undergoing in vitro fertilization.
abstract: OBJECTIVE
  To determine the relationship between progesterone receptor membrane component-1 (PGRMC1) expression and the outcome of IVF treatment.
  
  
  METHODS
  A prospective study in which PGRMC1 messenger RNA (mRNA) levels, methylation status of the Pgrmc1 promoter, and the presence of point mutations within Pgrmc1 were obtained from granulosa (GC)/luteal cells of women undergoing controlled ovarian hyperstimulation (COH).
  
  
  METHODS
  Fertility center/basic science laboratory.
  
  
  METHODS
  Eighty-five patients undergoing IVF treatment and 10 women who were undergoing COH for the purpose of oocyte donation were included in this study.
  
  
  METHODS
  None.
  
  
  METHODS
  The PGRMC1 measurements were correlated with clinical outcomes, such as number of follicles, number of retrieved oocytes, and ongoing pregnancy rates (PR).
  
  
  RESULTS
  The PGRMC1 mRNA levels within GC/luteal cells of 18% of IVF patients were >2.25-fold higher than those of oocyte donors. Individuals with elevated PGRMC1 mRNA levels had 30% fewer large follicles and fewer oocytes retrieved. The elevated PGRMC1 mRNA levels were associated with an increase in the methylation of Pgrmc1 promoter.
  
  
  CONCLUSIONS
  In patients with elevated PGRMC1 mRNA levels, gonadotropin-induced follicle development is attenuated, although sufficient numbers of follicles develop to allow for ET and subsequent pregnancy.
authors.affiliation: Department of Obstetrics and Gynecology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA.
authors.forename: Alyaa
authors.initials: A
authors.lastname: Elassar
authors.forename: Xiufang
authors.initials: X
authors.lastname: Liu
authors.forename: Victoria
authors.initials: V
authors.lastname: Scranton
authors.forename: Carol A
authors.initials: CA
authors.lastname: Wu
authors.forename: John J
authors.initials: JJ
authors.lastname: Peluso
mesh_terms: D000328:Adult; D003237:Connecticut; D019175:DNA Methylation; D004624:Embryo Transfer; D005091:Exons; D005260:Female; D005300:Fertility Agents, Female / Q000008:administration & dosage; D005307:Fertilization in Vitro; D006801:Humans; D007246:Infertility / Q000235:genetics / Q000378:metabolism / Q000628:therapy*; D008184:Luteal Cells / Q000187:drug effects / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D054315:Oocyte Retrieval; D010062:Ovulation Induction; D017354:Point Mutation; D011247:Pregnancy; D018873:Pregnancy Rate; D011401:Promoter Regions, Genetic; D011446:Prospective Studies; D012333:RNA, Messenger / Q000378:metabolism; D011980:Receptors, Progesterone / Q000235:genetics* / Q000378:metabolism; D016896:Treatment Outcome; D015854:Up-Regulation
chemical_list: D005300:Fertility Agents, Female; D008565:Membrane Proteins; C115296:PGRMC1 protein, human; D012333:RNA, Messenger; D011980:Receptors, Progesterone