title: A 1μm diameter tip fiber-based surface plasmon resonance system for single unit optical neural recording.
abstract: A gold-deposited optical fiber sensor system based on surface plasmon resonance (SPR) was developed for optical measurement of neuronal activity. To enhance the sensitivity of localized SPR and to make a precise and safe contact with the cellular membrane, we designed a tapered optical probe of 1 μm diameter at the tip of the fiber. By wet etching and gold evaporating processes, pencil-shaped optical probes were successfully fabricated. The SPR system with the sharp optical probe was integrated with a conventional patch clamping system to realize a simultaneous optical and electrical recording on a single neuron. Although the shape of optical signal is not clear due to tiny change of intrinsic optical properties on the neuron, optical and electrical signals were simultaneously changed by capsaicin stimulation. Furthermore, our designed fiber probe can be applicable to localized optical stimulation as well as in vivo optical neuroprosthetic devices.
authors.affiliation: School of Electrical Engineering and Computer Science, Seoul National University, Seoul, Republic of Korea. hwmoon88@gmail.com
authors.forename: Hyowon
authors.initials: H
authors.lastname: Moon
authors.forename: Shin Ae
authors.initials: SA
authors.lastname: Kim
authors.forename: Sang Beom
authors.initials: SB
authors.lastname: Jun
authors.forename: Jesun
authors.initials: J
authors.lastname: Lee
authors.forename: Uhtaek
authors.initials: U
authors.lastname: Oh
authors.forename: Sung June
authors.initials: SJ
authors.lastname: Kim
mesh_terms: D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005336:Fiber Optic Technology / Q000295:instrumentation*; D051379:Mice; D008904:Miniaturization; D009474:Neurons / Q000502:physiology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000295:instrumentation*; D056969:Voltage-Sensitive Dye Imaging / Q000295:instrumentation*