title: Biological, biochemical and molecular features of Trypanosoma cruzi strains isolated from patients infected through oral transmission during a 2005 outbreak in the state of Santa Catarina, Brazil: its correspondence with the new T. cruzi Taxonomy Consensus (2009).
abstract: We examined strains of Trypanosoma cruzi isolated from patients with acute Chagas disease that had been acquired by oral transmission in the state of Santa Catarina, Brazil (2005) and two isolates that had been obtained from a marsupial (Didelphis aurita) and a vector (Triatoma tibiamaculata). These strains were characterised through their biological behaviour and isoenzymic profiles and genotyped according to the new Taxonomy Consensus (2009) based on the discrete typing unities, that is, T. cruzi genotypes I-VI. All strains exhibited the biological behaviour of biodeme type II. In six isolates, late peaks of parasitaemia, beyond the 20th day, suggested a double infection with biodemes II + III. Isoenzymes revealed Z2 or mixed Z1 and Z2 profiles. Genotyping was performed using three polymorphic genes (cytochrome oxidase II, spliced leader intergenic region and 24Sα rRNA) and the restriction fragment length polymorphism of the kDNA minicircles. Based on these markers, all but four isolates were characterised as T. cruzi II genotypes. Four mixed populations were identified: SC90, SC93 and SC97 (T. cruzi I + T. cruzi II) and SC95 (T. cruzi I + T. cruzi VI). Comparison of the results obtained by different methods was essential for the correct identification of the mixed populations and major lineages involved indicating that characterisation by different methods can provide new insights into the relationship between phenotypic and genotypic aspects of parasite behaviour.
authors.affiliation: Laboratório de Chagas Experimental, Autoimunidade e Imunologia Celular, Centro de Pesquisas Gonçalo Moniz, Fiocruz, Salvador, BA, Brasil. sgandrade@bahia.fiocruz.br
authors.forename: Sonia Gumes
authors.initials: SG
authors.lastname: Andrade
authors.forename: Rozália Figueira
authors.initials: RF
authors.lastname: Campos
authors.forename: Mario
authors.initials: M
authors.lastname: Steindel
authors.forename: Marcos Lázaro
authors.initials: ML
authors.lastname: Guerreiro
authors.forename: Juracy Barbosa
authors.initials: JB
authors.lastname: Magalhães
authors.forename: Marcio Cerqueira de
authors.initials: MC
authors.lastname: Almeida
authors.forename: Joice Neves
authors.initials: JN
authors.lastname: Reis
authors.forename: Viviane Corrêa
authors.initials: VC
authors.lastname: Santos
authors.forename: Helder Magno Silva
authors.initials: HM
authors.lastname: Valadares
authors.forename: Mitermayer Galvão dos
authors.initials: MG
authors.lastname: Reis
authors.forename: Andréa Mara
authors.initials: AM
authors.lastname: Macedo
mesh_terms: D000818:Animals; D001938:Brazil / Q000453:epidemiology; D014355:Chagas Disease / Q000453:epidemiology / Q000469:parasitology* / Q000635:transmission; D032921:Consensus; D016054:DNA, Protozoan / Q000235:genetics; D048293:Didelphis / Q000469:parasitology; D004196:Disease Outbreaks; D004197:Disease Reservoirs / Q000469:parasitology; D005838:Genotype; D006801:Humans; D007303:Insect Vectors / Q000469:parasitology; D012335:RNA, Ribosomal / Q000235:genetics; D014224:Triatoma / Q000469:parasitology; D014349:Trypanosoma cruzi / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity
chemical_list: D016054:DNA, Protozoan; D012335:RNA, Ribosomal