title: Distribution of somatostatin receptor 5 in mouse and bullfrog retinas.
abstract: Somatostatin (SRIF), as a neuroactive peptide in the CNS, may act as a neuromodulator through activation of five specific receptor subtypes (sst(1)-sst(5)). In this work we conducted a comparative study of the expression of sst(5) in mouse and bullfrog retinas by immunofluorescence double labeling. Basically, the expression profiles of sst(5) in the retinas of the two species were similar. That is, in the inner retina sst(5) was localized to dopaminergic and cholinergic amacrine cells, stained by tyrosine hydroxylase (TH) and choline acetyltransferase (ChAT) respectively, and cells in the ganglion cell layer, whereas in the outer retina immunostaining for sst(5) was observed in horizontal cells. However, a more widespread, abundant distribution of labeling for sst(5), as compared to mouse retina, was seen in bullfrog retina: strong labeling for sst(5) was diffusely distributed in both outer and inner plexiform layers (OPL and IPL) in the bullfrog retina, but the labeling was only observed in the IPL of the mouse retina. In addition, bullfrog photoreceptors, both rods and cones, but not mouse ones, were labeled by sst(5). In combination with the experiments showing that SRIF-immunoreactivity was mainly found in the inner retina, our results suggest that SRIF, released from SRIF-containing cells in the inner retina, may play a neuromodulatory role in both outer and inner retina mediated by volume transmission via sst(5) in bullfrog retina, while the SRIF action may be largely restricted to the mouse inner retina.
authors.affiliation: Institute of Neurobiology, Institutes of Brain Science and State Key Laboratory of Medical Neurobiology, Fudan University, 138 Yixueyuan Road, Shanghai 200032, China.
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Wu
authors.forename: Qin-Qin
authors.initials: QQ
authors.lastname: Deng
authors.forename: Shi-Xiang
authors.initials: SX
authors.lastname: Jiang
authors.forename: Xiong-Li
authors.initials: XL
authors.lastname: Yang
authors.forename: Yong-Mei
authors.initials: YM
authors.lastname: Zhong
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D015870:Gene Expression; D020869:Gene Expression Profiling; D008297:Male; D051379:Mice / Q000033:anatomy & histology*; D008810:Mice, Inbred C57BL; D009928:Organ Specificity; D020419:Photoreceptor Cells, Vertebrate / Q000378:metabolism; D011892:Rana catesbeiana / Q000033:anatomy & histology*; D017481:Receptors, Somatostatin / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D012160:Retina / Q000166:cytology / Q000378:metabolism*; D012165:Retinal Ganglion Cells / Q000378:metabolism
chemical_list: D017481:Receptors, Somatostatin; C094573:somatostatin receptor 5