title: Multiplexed, rapid point of care platform to quantify allergen-specific IgE.
abstract: Variation of probe immobilization on microarrays hinders the ability to make high quality, assertive and statistically relevant conclusions needed in the healthcare setting. To address this problem, we have developed a calibrated, compact, inexpensive, multiplexed, dual modality point-of-care detection platform that calibrates and correlates surface probe density measured label-free to captured labeled secondary antibody, is independent of chip-to-chip variability, and improves upon existing diagnostic technology. We have identified four major technological advantages of our proposed platform: the capability to perform single spot analysis based on the fluorophore used for detection, a 10-fold gain in fluorescence signal due to optimized substrate, a calibrated, quantitative method that uses the combined fluorescent and label-free modalities to accurately measure the density of probe and bound target for a variety of systems, and a compact measurement platform offering reliable and rapid results at the doctor's office. Already, we have formulated over a 90% linear correlation between the amount of probe bound to surface and the resulting fluorescence of captured target for IgG, β-lactoglobulin, Ara h 1 peanut allergen, and Phl 5a Timothy grass allergen.
authors.affiliation: Biomedical Engineering Department BostonUniversity, Boston, Ma 02446, USA.
authors.forename: M R
authors.initials: MR
authors.lastname: Monroe
authors.forename: A P
authors.initials: AP
authors.lastname: Reddington
authors.forename: M
authors.initials: M
authors.lastname: Cretich
authors.forename: M
authors.initials: M
authors.lastname: Chiari
authors.forename: F
authors.initials: F
authors.lastname: Little
authors.forename: M S
authors.initials: MS
authors.lastname: Ünlü
mesh_terms: D000485:Allergens / Q000032:analysis* / Q000276:immunology*; D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D007073:Immunoglobulin E / Q000032:analysis* / Q000276:immunology*; D008904:Miniaturization; D019095:Point-of-Care Systems; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation
chemical_list: D000485:Allergens; D007073:Immunoglobulin E