title: Prognostic implications of microRNA-100 and its functional roles in             human epithelial ovarian cancer.
abstract: Dysregulation of microRNAs (miRNAs) has been found to be associated with             a variety of diseases, including epithelial ovarian cancer (EOC). Recently, miR-100             was reported to be downregulated in human ovarian carcinoma, however, the clinical             significance and functional roles of miR-100 expression in human EOC are unclear.             TaqMan real-time quantitative RT-PCR assay was performed to detect the expression             of miR-100 in 98 EOC tissues and 15 adjacent normal epithelial tissues. The relationship             between miR-100 expression and clinicopathological factors in 98 EOC patients             was statistically analyzed. The effect of miR-100 expression on patient survival             was determined. Finally, the role of miR-100 in EOC cell growth and its possible             mechanisms were analyzed with miR-100 precursor or inhibitor-transfected cells.             We showed that the level of miR-100 was significantly lower in EOC tissues compared             to adjacent normal tissues. Low miR-100 expression was found to be closely correlated             with advanced FIGO stage, higher serum CA125 expression level and lymph node involvement.             Also, low miR-100 expression was correlated with shorter overall survival of EOC             patients, and multivariate analysis showed that the status of miR-100 expression             was an independent predictor of overall survival in EOC. Additionally, miR-100             could affect the growth of EOC cells by post-transcriptionally regulating polo-like             kinase 1 (PLK1) expression. Together, these results suggest that low miR-100 expression             may be an independent poor prognostic factor and miR-100 can function as a tumor             suppressor by targeting PLK1 in human EOCs.
authors.affiliation: Department of Obstetrics and Gynecology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, PR China. pdx_gz@163.com
authors.forename: Dong-Xian
authors.initials: DX
authors.lastname: Peng
authors.forename: Min
authors.initials: M
authors.lastname: Luo
authors.forename: Li-Wen
authors.initials: LW
authors.lastname: Qiu
authors.forename: Yuan-Li
authors.initials: YL
authors.lastname: He
authors.forename: Xue-Feng
authors.initials: XF
authors.lastname: Wang
mesh_terms: D018394:CA-125 Antigen / Q000097:blood; D000077216:Carcinoma, Ovarian Epithelial; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002681:China; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008207:Lymphatic Metastasis; D008565:Membrane Proteins / Q000097:blood; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D015999:Multivariate Analysis; D009367:Neoplasm Staging; D009375:Neoplasms, Glandular and Epithelial / Q000235:genetics* / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D011379:Prognosis; D016016:Proportional Hazards Models; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D060888:Real-Time Polymerase Chain Reaction; D018570:Risk Assessment; D012307:Risk Factors; D013997:Time Factors; D014162:Transfection; D000097869:Polo-Like Kinase 1
chemical_list: D018394:CA-125 Antigen; D018797:Cell Cycle Proteins; D008565:Membrane Proteins; D035683:MicroRNAs; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D000097869:Polo-Like Kinase 1; C544266:MIRN100 microRNA, human; C451759:MUC16 protein, human