title: IGF-1 deficiency in combination with a low basic hBD-2 and hBD-3 gene expression might counteract malignant transformation in pleomorphic adenomas in vitro.
abstract: This study investigated the IGF-1-influence on oncological relevant genes in pleomorphic adenomas. Therefore A64-tumor cells were stimulated by recombinant IGF-1. After RNA-extraction, transcript levels of hBD-1, hBD-2, hBD-3, DEFA1/3, DEFA4, S100A4, Psoriasin, DOC-1, EGF, EGFR, and IGFR were analyzed by qRT-PCR at t = 0, 4, 8, 24, 48, and 72 hr. The gene-products were visualized by immunostaining. A64-tumor-cells were deficient for hBD-1 and IGF-1. IGF-1 downregulates hBD-2 and hBD-3 without influencing hBD-1-expression. IGF-1 only slightly affects DEFA1/3-, DEFA4-, S100A4-, Psoriasin-, DOC-1-, EGF-, EGFR-, and IGFR-gene-expression. IGF-1-deficiency combined with low basic hBD-2-gene-expression and hBD-3-gene-expression might counteract, whereas hBD-1-deficiency promotes malignant transformation in pleomorphic adenomas.
authors.affiliation: Department of Periodontology, Operative and Preventive Dentistry, University of Bonn, Welschnonnenstr, Bonn, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Winter
authors.forename: S
authors.initials: S
authors.lastname: Mohr
authors.forename: A
authors.initials: A
authors.lastname: Pantelis
authors.forename: D
authors.initials: D
authors.lastname: Kraus
authors.forename: J P
authors.initials: JP
authors.lastname: Allam
authors.forename: N
authors.initials: N
authors.lastname: Novak
authors.forename: R
authors.initials: R
authors.lastname: Reich
authors.forename: M
authors.initials: M
authors.lastname: Martini
authors.forename: S
authors.initials: S
authors.lastname: Jepsen
authors.forename: W
authors.initials: W
authors.lastname: Götz
authors.forename: M
authors.initials: M
authors.lastname: Wenghoefer
mesh_terms: D008949:Adenoma, Pleomorphic / Q000235:genetics* / Q000378:metabolism; D048708:Cell Growth Processes / Q000502:physiology; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D007334:Insulin-Like Growth Factor I / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D011994:Recombinant Proteins / Q000494:pharmacology; D012468:Salivary Gland Neoplasms / Q000235:genetics* / Q000378:metabolism; D023083:beta-Defensins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D023083:beta-Defensins; D007334:Insulin-Like Growth Factor I