title: Evaluation of minichromosome maintenance protein 7 as a prognostic marker in canine cutaneous mast cell tumours.
abstract: Minichromosome maintenance proteins (MCMs) are sensitive markers of cellular proliferation and have been shown to be significant predictors of survival in several human malignancies. MCM7 was evaluated as a prognostic marker in canine cutaneous mast cell tumours (MCTs). MCM7 immunohistochemistry was performed and an index of MCM7-positive cells calculated in dogs with known outcome. The Receiver Operating Characteristics method was used to individuate the best cut-off value of MCM7 score as predictor of survival. Survival analysis and prognostic variables were analysed with statistical methods. Ninety-five dogs were included with 31 dying of MCTs. A value of 0.18 was used as cut-off value of MCM7 score as a binary variable. The median survival time for MCM7 score ≤0.18 was not reached at 3668 days, whereas for MCM7 score >0.18 was 187 days (log-rank test; P < 0.0001). In the multivariable analysis, MCM7 was significantly associated with survival after controlling for age, surgical margins and histological grade (hazard ratio 9.2; P = 0.001).
authors.affiliation: Oncology Unit, Animal Health Trust, Newmarket, UK. davide.berlato@aht.org.uk
authors.forename: D
authors.initials: D
authors.lastname: Berlato
authors.forename: J
authors.initials: J
authors.lastname: Stewart
authors.forename: R
authors.initials: R
authors.lastname: Newton
authors.forename: G A
authors.initials: GA
authors.lastname: Maglennon
authors.forename: P
authors.initials: P
authors.lastname: Monti
authors.forename: A
authors.initials: A
authors.lastname: Flindall
authors.forename: S
authors.initials: S
authors.lastname: Murphy
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004283:Dog Diseases / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D004285:Dogs; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D008297:Male; D054705:Mastocytoma, Skin / Q000378:metabolism / Q000401:mortality / Q000662:veterinary*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D011379:Prognosis; D012189:Retrospective Studies
chemical_list: D014408:Biomarkers, Tumor; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins