title: [Correlation between TCID50 and p27 antigen of avian leukosis virus subgroup J].
abstract: To study the correlation between 50% tissue-culture infective dose (TCID50) value and p27 antigen S/P value of Avian leukosis virus subgroup J and discuss their significance, chicken embryo fibroblast (CEF) cells were inoculated with Avian leukosis virus subgroup J strain NX0101 and samples were tested continuously for ten days after changing maintenance media. The correlation between TCID50 and p27 antigen S/P value of ten days were then analysized. Simultaneously, DF-1 cells were inoculated with NX0101 and passaged to 20 generations. Samples taken from 1st generation, 5th generation, 10th generation, 15th generation and 20th generation were tested for the TCID50 titer and the p27 antigen S/P value separately. A significant Pearson correlation was found between them in CEF cells (r = 0.85277; P < 0.0001) and in DF-1 cells (r = 0.93000; P = 0.0220). This study provided an important parameter for predicting TCID50 by detecting the p27 antigen S/P value.
authors.affiliation: College of Veterianry Medicine, Shandong Agricultural University, Taian 271018, China. dongxuan311@163.com
authors.forename: Xuan
authors.initials: X
authors.lastname: Dong
authors.forename: Juan
authors.initials: J
authors.lastname: Liu
authors.forename: Peng
authors.initials: P
authors.lastname: Zhao
authors.forename: Shuai
authors.initials: S
authors.lastname: Su
authors.forename: Yan
authors.initials: Y
authors.lastname: Du
authors.forename: Xue
authors.initials: X
authors.lastname: Li
authors.forename: Zhi-Zhong
authors.initials: ZZ
authors.lastname: Cui
mesh_terms: D000818:Animals; D001353:Avian Leukosis / Q000821:virology; D001354:Avian Leukosis Virus / Q000276:immunology* / Q000472:pathogenicity*; D002642:Chick Embryo; D005347:Fibroblasts / Q000821:virology; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis / Q000276:immunology*; D019562:Viral Load / Q000276:immunology
chemical_list: D018809:Proliferating Cell Nuclear Antigen; C413374:p27 antigen