title: [A GeXP based multiplex RT-PCR assay for simultaneous detection of twelve human respiratory viruses].
abstract: A GeXP based multiplex RT-PCR assay was developed to simultaneously detect twelve different respiratory viruses types/subtypes including influenza A virus, influenza B virus, influenza A virus sH1N1, parainfluenza virus type 1, parainfluenza virus type 2, parainfluenza virus type 3, human rhinovirus, human metapneumovirus, adenovirus, respiratory syncytial virus A, respiratory syncytial virus B and human bocavirus. Twelve sets of specific primers were designed based on the conserved sequences of available respiratory-virus sequence database. The specificity of the multiplex system was examined by positive specimens confirmed previously. The sensitivity to detect twelve respiratory viruses simultaneously was 10(3) copies/microL. Twenty four clinical specimens were further detected by this novel assay and the results were compared with that of the real-time RT-PCR. These results showed that this novel assay based on GeXP is a fast, sensitive, and high throughput test for the detection of respiratory virus infections.
authors.affiliation: State Key Laboratory for Genetic Engineering and Molecular Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Li
authors.forename: Nai-Ying
authors.initials: NY
authors.lastname: Mao
authors.forename: Meng
authors.initials: M
authors.lastname: Qin
authors.forename: Xiu-Mei
authors.initials: XM
authors.lastname: Hu
authors.forename: Meng-Jie
authors.initials: MJ
authors.lastname: Yang
authors.forename: Miao
authors.initials: M
authors.lastname: Wang
authors.forename: Chen
authors.initials: C
authors.lastname: Zhang
authors.forename: Wen-Bo
authors.initials: WB
authors.lastname: Xu
authors.forename: Xue-Jun
authors.initials: XJ
authors.lastname: Ma
mesh_terms: D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000302:isolation & purification; D009975:Orthomyxoviridae / Q000235:genetics / Q000302:isolation & purification; D009976:Orthomyxoviridae Infections / Q000821:virology; D012328:RNA Viruses / Q000235:genetics / Q000302:isolation & purification*; D060888:Real-Time Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*; D012136:Respiratory Syncytial Viruses / Q000235:genetics / Q000302:isolation & purification; D012141:Respiratory Tract Infections / Q000821:virology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*; D012229:Rhinovirus / Q000235:genetics / Q000302:isolation & purification; D012680:Sensitivity and Specificity