title: Promoting self-assembly of collagen-related peptides into various higher-order structures by metal-histidine coordination.
abstract: Collagen is an important and widely used biomaterial and therapeutic. The construction of large-scale collagen structures via the self-assembly of small collagen-related peptides has been extensively studied in the past decade. Here, we report a highly effective and simple means to assemble small synthetic collagen-related peptides into various higher-order structures by utilizing metal-histidine coordination. In this work, two short collagen-related peptides in which histidine residues were incorporated as metal binding sites were designed and chemically synthesized: HG(PPG)(9)GH (X9) and HG(PPG)(4)(PHG)(PPG)(4)GH (PHG). Circular dichroism measurements indicated that these two peptides form only marginally stable collagen triple helices but that their stability can be increased upon the addition of metal ions. Dynamic light scattering analyses, turbidity measurements, TEM, and SEM results demonstrated the metal ion-dependent self-assembly of X9 and PHG into supramolecular structures ranging from various nanofibrils to microscale spherical, laminated, and granulated assemblies. The topology and size of these higher-order structures depends both on the metal ion identity and the location of the binding sites. Most intriguingly, the assembled fibrils show similar D-periodicity to that of natural collagen. Our results demonstrate that metal-histidine coordination can serve as an effective force to induce the self-assembly of unstable collagen-related peptides into higher-order structures.
authors.affiliation: Department of Chemistry, National Tsing Hua University, Hsinchu, Taiwan 30013, ROC.
authors.forename: Wei
authors.initials: W
authors.lastname: Hsu
authors.forename: Yi-Lun
authors.initials: YL
authors.lastname: Chen
authors.forename: Jia-Cherng
authors.initials: JC
authors.lastname: Horng
mesh_terms: D003094:Collagen / Q000737:chemistry*; D006639:Histidine / Q000737:chemistry*; D008670:Metals / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D057139:Nanofibers / Q000737:chemistry / Q000648:ultrastructure; D010455:Peptides / Q000737:chemistry*
chemical_list: D008670:Metals; D010455:Peptides; D006639:Histidine; D003094:Collagen