title: DNMT3B (C46359T) polymorphisms and immunoexpression of DNMT3b and DNMT1 proteins in oral lichen planus.
abstract: OBJECTIVE
  To investigate the DNMT3B (C46359T) polymorphism and immunoexpression of DNMT3b and DNMT1 in oral lichen planus (OLP) compared to a control group.
  
  
  METHODS
  We aimed to investigate the DNMT3B (C46359T) polymorphism and immunoexpression of DNMT3b and DNMT1 in OLP (n = 32), comparing it with oral mucosa (control; n = 24). The DNMT3B (C46359T) polymorphism was analyzed using the RFLP-PCR and DNMT1, and DNMT3a proteins were identified using immunohistochemistry. We also compared the DNMT3B expression in OLP and oral inflammatory fibrous hyperplasia (OIFH), another oral inflammatory disease. Differences between the groups were determined by specific statistical analyses.
  
  
  RESULTS
  The CT genotype of DNMT3B was associated with OLP development (p = 0.012). Increased expression of DNMT3B and DNMT1 was observed in OLP compared to the control group (p = 0.014 and p = 0.001, respectively). A significant increase in DNMT3B protein levels was observed in the genotype CT in DNMT3B (C46359T) polymorphisms (p = 0.045). No DNMT3B expression differences between OLP and OIFH were observed.
  
  
  CONCLUSIONS
  Our data show that the DNMT3B (C46359T) polymorphism is associated with OLP development. Furthermore, increased expression of the enzyme DNMT3B, an epigenetic-associated protein, is present in OLP.
authors.affiliation: Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Brazil.
authors.forename: Thiago
authors.initials: T
authors.lastname: Fonseca-Silva
authors.forename: Marcos Vinícius Macedo de
authors.initials: MV
authors.lastname: Oliveira
authors.forename: Carlos Alberto de Carvalho
authors.initials: CA
authors.lastname: Fraga
authors.forename: Lucyana Conceição
authors.initials: LC
authors.lastname: Farias
authors.forename: Erika Patrícia Pereira
authors.initials: EP
authors.lastname: Gomes
authors.forename: Lucas Oliveira
authors.initials: LO
authors.lastname: Barros
authors.forename: Ashbeel
authors.initials: A
authors.lastname: Roy
authors.forename: Ricardo
authors.initials: R
authors.lastname: Santiago Gomez
authors.forename: Alfredo Maurício Batista De
authors.initials: AM
authors.lastname: Paula
authors.forename: André Luiz Sena
authors.initials: AL
authors.lastname: Guimarães
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D000090205:DNA Methyltransferase 3A; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D017676:Lichen Planus, Oral / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D012097:Repressor Proteins / Q000276:immunology / Q000378:metabolism*; D055815:Young Adult; D000097584:DNA Methyltransferase 3B
chemical_list: D004248:DNA (Cytosine-5-)-Methyltransferases; D000090205:DNA Methyltransferase 3A; D012097:Repressor Proteins; D000097584:DNA Methyltransferase 3B; C411744:DMAP1 protein, human; C000717967:DNMT3A protein, human