title: Site-specific recombination strategies for engineering actinomycete genomes.
abstract: The feasibility of using technologies based on site-specific recombination in actinomycetes was shown several years ago. Despite their huge potential, these technologies mostly have been used for simple marker removal from a chromosome. In this paper, we present different site-specific recombination strategies for genome engineering in several actinomycetes belonging to the genera Streptomyces, Micromonospora, and Saccharothrix. Two different systems based on Cre/loxP and Dre/rox have been utilized for numerous applications. The activity of the Cre recombinase on the heterospecific loxLE and loxRE sites was similar to its activity on wild-type loxP sites. Moreover, an apramycin resistance marker flanked by the loxLERE sites was eliminated from the Streptomyces coelicolor M145 genome at a surprisingly high frequency (80%) compared to other bacteria. A synthetic gene encoding the Dre recombinase was constructed and successfully expressed in actinomycetes. We developed a marker-free expression method based on the combination of phage integration systems and site-specific recombinases. The Cre recombinase has been used in the deletion of huge genomic regions, including the phenalinolactone, monensin, and lipomycin biosynthetic gene clusters from Streptomyces sp. strain Tü6071, Streptomyces cinnamonensis A519, and Streptomyces aureofaciens Tü117, respectively. Finally, we also demonstrated the site-specific integration of plasmid and cosmid DNA into the chromosome of actinomycetes catalyzed by the Cre recombinase. We anticipate that the strategies presented here will be used extensively to study the genetics of actinomycetes.
authors.affiliation: Albert Ludwigs University of Freiburg, Department of Pharmaceutical Biology and Biotechnology, Freiburg, Germany.
authors.forename: Simone
authors.initials: S
authors.lastname: Herrmann
authors.forename: Theresa
authors.initials: T
authors.lastname: Siegl
authors.forename: Marta
authors.initials: M
authors.lastname: Luzhetska
authors.forename: Lutz
authors.initials: L
authors.lastname: Petzke
authors.forename: Caroline
authors.initials: C
authors.lastname: Jilg
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Welle
authors.forename: Annette
authors.initials: A
authors.lastname: Erb
authors.forename: Peter F
authors.initials: PF
authors.lastname: Leadlay
authors.forename: Andreas
authors.initials: A
authors.lastname: Bechthold
authors.forename: Andriy
authors.initials: A
authors.lastname: Luzhetskyy
mesh_terms: D000192:Actinomycetales / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods*; D005827:Genetics, Microbial / Q000379:methods*; D016680:Genome, Bacterial; D008967:Molecular Biology / Q000379:methods*; D016254:Mutagenesis, Insertional; D010957:Plasmids; D045522:Recombinases / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic; D017384:Sequence Deletion
chemical_list: D045522:Recombinases