title: Comparison of midgut bacterial diversity in tropical caterpillars (Lepidoptera: Saturniidae) fed on different diets.
abstract: As primary consumers of foliage, caterpillars play essential roles in shaping the trophic structure of tropical forests. The caterpillar midgut is specialized in plant tissue processing; its pH is exceptionally alkaline and contains high concentrations of toxic compounds derived from  the ingested plant material (secondary compounds or allelochemicals) and from the insect itself. The midgut, therefore, represents an extreme environment for microbial life. Isolates from different bacterial taxa have been recovered from caterpillar midguts, but little is known about the impact  of these microorganisms on caterpillar biology. Our long-term goals are to identify midgut symbionts and to investigate their functions. As a first step, different diet formulations were evaluated for rearing two species of tropical saturniid caterpillars. Using the polymerase chain reaction  (PCR) with primers hybridizing broadly to sequences from the bacterial domain, 16S rRNA gene libraries were constructed with midgut DNA extracted from caterpillars reared on different diets. Amplified rDNA restriction analysis indicated that bacterial sequences recovered from the midguts of  caterpillars fed on foliage were more diverse than those from caterpillars fed on artificial diet. Sequences related to Methylobacterium sp., Bradyrhizobium sp., and Propionibacterium sp. were detected in all caterpillar libraries regardless of diet, but were not detected in a library constructed  from the diet itself. Furthermore, libraries constructed with DNA recovered from surface-sterilized eggs indicated potential for vertical transmission of midgut symbionts. Taken together, these results suggest that microorganisms associated with the tropical caterpillar midgut may engage in  symbiotic interactions with these ecologically important insects.
authors.affiliation: Department of Plant Pathology, University of Wisconsin, Madison, WI 53706, USA.
authors.forename: Adrián A
authors.initials: AA
authors.lastname: Pinto-Tomás
authors.forename: Ana
authors.initials: A
authors.lastname: Sittenfeld
authors.forename: Lorena
authors.initials: L
authors.lastname: Uribe-Lorío
authors.forename: Felipe
authors.initials: F
authors.lastname: Chavarría
authors.forename: Marielos
authors.initials: M
authors.lastname: Mora
authors.forename: Daniel H
authors.initials: DH
authors.lastname: Janzen
authors.forename: Robert M
authors.initials: RM
authors.lastname: Goodman
authors.forename: Holly M
authors.initials: HM
authors.lastname: Simon
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification / Q000235:genetics*; D004032:Diet; D005260:Female; D041981:Gastrointestinal Tract / Q000382:microbiology; D007814:Larva / Q000254:growth & development / Q000382:microbiology; D008297:Male; D009036:Moths / Q000254:growth & development / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012727:Sex Characteristics; D013559:Symbiosis
chemical_list: D012336:RNA, Ribosomal, 16S