title: Characterization of channel-forming peptide nanostructures.
abstract: We have prepared fluorescent analogs of known ion-channel-forming synthetic peptide nanostructures. These analogs were designed as probes to gain insight about the mechanism by which self-assembling amphiphilic peptides interact with lipid membranes. Conformational studies demonstrated that the labeled analogs retain their propensity to adopt a strong helical conformation in 2,2,2-trifluoroethanol and lipid bilayers. Attenuated total reflectance results indicated that the fluorescent peptide nanostructures are under an incorporation equilibrium between two forms, adsorbed at the surface or incorporated within the bilayer, similar to their unlabeled counterparts. However, when using a HeLa mimicking membrane, the proportion of peptide nanostructures in the transmembrane orientation decreases significantly. Finally, we were able to show by confocal microscopy studies that fluorescent analogs internalized into HeLa cells and localized into both the membranes of inner organelles and the cell membrane.
authors.affiliation: PROTEO, Quebec Research Network on Protein Structure, Function and Engineering, Université Laval, Quebec City, Canada.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Arseneault
authors.forename: Michel
authors.initials: M
authors.lastname: Dumont
authors.forename: François
authors.initials: F
authors.lastname: Otis
authors.forename: Normand
authors.initials: N
authors.lastname: Voyer
mesh_terms: D002462:Cell Membrane / Q000378:metabolism; D002463:Cell Membrane Permeability; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D007473:Ion Channels / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism; D049329:Nanostructures / Q000032:analysis / Q000737:chemistry*; D010455:Peptides / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D017433:Protein Structure, Secondary; D014270:Trifluoroethanol / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D007473:Ion Channels; D008051:Lipid Bilayers; D010455:Peptides; D014270:Trifluoroethanol