title: Delivery of MicroRNA-10b with Polylysine Nanoparticles for Inhibition of Breast Cancer Cell Wound Healing.
abstract: Recent studies revealed that micro RNA-10b (mir-10b) is highly expressed in metastatic breast cancer cells and positively regulates breast cancer cell migration and invasion through inhibition of HOXD10 target synthesis. In this study we designed anti-mir-10b molecules and combined them with poly L-lysine (PLL) to test the delivery effectiveness. An RNA molecule sequence exactly matching the mature mir-10b minor antisense showed strong inhibition when mixed with PLL in a wound-healing assay with human breast cell line MDA-MB-231. The resulting PLL-RNA nanoparticles delivered the anti-microRNA molecules into cytoplasm of breast cancer cells in a concentration-dependent manner that displayed sustainable effectiveness.
authors.affiliation: Postdoctoral Research Associate, Cell Biology and Biochemistry Group, Fundamental and Computational Sciences Directorate, Pacific Northwest National Laboratory, Richland WA, USA.
authors.forename: Hongjun
authors.initials: H
authors.lastname: Jin
authors.forename: Yuehua
authors.initials: Y
authors.lastname: Yu
authors.forename: William B
authors.initials: WB
authors.lastname: Chrisler
authors.forename: Yijia
authors.initials: Y
authors.lastname: Xiong
authors.forename: Dehong
authors.initials: D
authors.lastname: Hu
authors.forename: Chenghong
authors.initials: C
authors.lastname: Lei