title: Using photonic crystal enhanced fluorescence on quartz substrates to improve the sensitivity of DNA microarrays.
abstract: Gene expression analysis of low abundance genes remains difficult when DNA microarrays are performed on standard glass substrates. However, we have shown that by using photonic crystals (PC) made on quartz substrates, the fluorescence intensity of Cyanine-5 (Cy5) labeled microarray spots is greatly enhanced. In a 1-color microarray experiment studying gene expression of soybean cotyledon tissue, an average signal enhancement factor of 17.8× was observed on the PC. Furthermore, twice as many genes were detectable on these PCs as compared to glass. By improving the sensitivity of this fluorescent assay, low expression genes that were undetectable on glass were quantified on the PC.
authors.affiliation: Department of Bioengineering, University of Illinois Urbana Champaign, IL 61801, USA.
authors.forename: Sherine
authors.initials: S
authors.lastname: George
authors.forename: Anusha
authors.initials: A
authors.lastname: Pokhriyal
authors.forename: Sarah I
authors.initials: SI
authors.lastname: Jones
authors.forename: Meng
authors.initials: M
authors.lastname: Lu
authors.forename: Lila O
authors.initials: LO
authors.lastname: Vodkin
authors.forename: Brian T
authors.initials: BT
authors.lastname: Cunningham
mesh_terms: D003460:Crystallization; D004867:Equipment Design; D019544:Equipment Failure Analysis; D017404:In Situ Hybridization, Fluorescence / Q000295:instrumentation*; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation*; D017785:Photons; D011791:Quartz / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D011791:Quartz