title: Improving signal-to-noise ratio of structured light microscopy based on photon reassignment.
abstract: In this paper, we report a method for 3D visualization of a biological specimen utilizing a structured light wide-field microscopic imaging system. This method improves on existing structured light imaging modalities by reassigning fluorescence photons generated from off-focal plane excitation, improving in-focus signal strength. Utilizing a maximum likelihood approach, we identify the most likely fluorophore distribution in 3D that will produce the observed image stacks under structured and uniform illumination using an iterative maximization algorithm. Our results show the optical sectioning capability of tissue specimens while mostly preserving image stack photon count, which is usually not achievable with other existing structured light imaging methods.
authors.affiliation: BioSystems and Micromechanics IRG, Singapore-MIT Alliance for Research and Technology (SMART) centre, 3 Science Drive 2, 117543, Singapore.
authors.forename: Vijay Raj
authors.initials: VR
authors.lastname: Singh
authors.forename: Heejin
authors.initials: H
authors.lastname: Choi
authors.forename: Elijah Y S
authors.initials: EY
authors.lastname: Yew
authors.forename: Dipanjan
authors.initials: D
authors.lastname: Bhattacharya
authors.forename: Luo
authors.initials: L
authors.lastname: Yuan
authors.forename: Colin J R
authors.initials: CJ
authors.lastname: Sheppard
authors.forename: Jagath C
authors.initials: JC
authors.lastname: Rajapakse
authors.forename: George
authors.initials: G
authors.lastname: Barbastathis
authors.forename: Peter T C
authors.initials: PT
authors.lastname: So