title: Inhibition of infectious bursal disease virus infection by artificial microRNAs targeting chicken heat-shock protein 90.
abstract: Infectious bursal disease virus (IBDV) causes an important disease in young chickens. Chicken heat-shock protein 90 (cHsp90) has been shown to be a functional component of the cellular receptor complex for IBDV infection. This study demonstrates the inhibitory effect of vector-expressed anti-cHsp90α microRNA (miRNA) on IBDV infection. The reporter vectors pcHsp90α-EGFP and pcHsp90β-EGFP were constructed to facilitate effective miRNA selection. Two anti-cHsp90α and one anti-cHsp90β miRNA-expression vectors were constructed for a stable transfection study. Poly(A)-tailed RT-PCR detected sequence-specific miRNA transcription in transfected cells. Semiquantitative RT-PCR showed inhibition of cHsp90 transcription in transfected cells. A virus-titration assay showed that the anti-cHsp90α miRNA, but not the anti-cHsp90β miRNA, had inhibitory effects on IBDV infection. These results suggest that cHsp90α is a functional component of the cellular receptor complex for IBDV infection, and that anti-cHsp90α miRNA could be used as an anti-IBDV reagent.
authors.affiliation: College of Veterinary Medicine, Yangzhou University, 48 Wenhui Road, Yangzhou 225009, PR China.
authors.forename: Weifeng
authors.initials: W
authors.lastname: Yuan
authors.affiliation: College of Veterinary Medicine, Yangzhou University, 48 Wenhui Road, Yangzhou 225009, PR China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Yangzhou University, 48 Wenhui Road, Yangzhou 225009, PR China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Xia
authors.affiliation: College of Veterinary Medicine, Yangzhou University, 48 Wenhui Road, Yangzhou 225009, PR China.
authors.forename: Huaichang
authors.initials: H
authors.lastname: Sun
mesh_terms: D000818:Animals; D018175:Birnaviridae Infections / Q000235:genetics / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D002460:Cell Line; D002645:Chickens / Q000235:genetics / Q000821:virology; D018841:HSP90 Heat-Shock Proteins / Q000037:antagonists & inhibitors / Q000235:genetics*; D007243:Infectious bursal disease virus / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics / Q000627:therapeutic use*; D016133:Polymerase Chain Reaction; D011201:Poultry Diseases / Q000235:genetics / Q000517:prevention & control* / Q000821:virology; D011991:Receptors, Virus / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects / Q000235:genetics; D014162:Transfection
chemical_list: D018841:HSP90 Heat-Shock Proteins; D035683:MicroRNAs; D011991:Receptors, Virus