title: Novel micro-extraction by packed sorbent procedure for the liquid chromatographic analysis of antiepileptic drugs in human plasma and urine.
abstract: A method for the simultaneous determination of the antiepileptic drugs, phenobarbital (PHB), phenytoin (PTN), carbamazepine (CBZ), primidone (PRM) and oxcarbazepine (OXC) in human plasma and urine samples by using micro-extraction in a packed syringe as the sample preparation method connected with LC/UV (MEPS/LC/UV) is described. Micro-extraction in a packed syringe (MEPS) is a new miniaturized, solid-phase extraction technique that can be connected online to gas or liquid chromatography without any modifications. In MEPS approximately 1 mg of the solid packing material is inserted into a syringe (100-250 μL) as a plug. Sample preparation takes place on the packed bed. The bed can be coated to provide selective and suitable sampling conditions. The new method is very promising, easy to use, fully automated, inexpensive and quick. The standard curves were obtained within the concentration range 1-500 ng/mL in both plasma and urine samples. The results showed high correlation coefficients (R(2) >0.988) for all of the analytes within the calibration range. The extraction recovery was found to be between 88.56 and 99.38%. The limit of quantification was found to be between 0.132 and 1.956 ng/mL. The precision (RSD) values of quality control samples (QC) had a maximum deviation of 4.9%. A comparison of the detection limits with similar methods indicates high sensitivity of the present method. The method is applied for the analysis of these drugs in real urine and plasma samples of epileptic patients.
authors.affiliation: Punjabi University, Department of Chemistry, Patiala, India.
authors.forename: Susheela
authors.initials: S
authors.lastname: Rani
authors.forename: Ashok K
authors.initials: AK
authors.lastname: Malik
authors.forename: Baldev
authors.initials: B
authors.lastname: Singh
mesh_terms: D000927:Anticonvulsants / Q000097:blood* / Q000737:chemistry / Q000652:urine*; D002220:Carbamazepine / Q000031:analogs & derivatives / Q000097:blood / Q000737:chemistry / Q000652:urine; D002853:Chromatography, Liquid; D006801:Humans; D000078330:Oxcarbazepine; D010634:Phenobarbital / Q000097:blood / Q000737:chemistry / Q000652:urine; D010672:Phenytoin / Q000097:blood / Q000737:chemistry / Q000652:urine; D011324:Primidone / Q000097:blood / Q000737:chemistry / Q000652:urine; D000091402:Prohibitins; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000927:Anticonvulsants; C000717328:PHB protein, human; D000091402:Prohibitins; D011324:Primidone; D002220:Carbamazepine; D010672:Phenytoin; D000078330:Oxcarbazepine; D010634:Phenobarbital