title: Case study: targeted high-throughput sequencing of mitochondrial genomes from extinct cave bears via direct multiplex PCR sequencing (DMPS).
abstract: Here I describe the use of a recently developed technique for targeted high-throughput sequencing of highly degraded DNA by direct multiplex PCR sequencing (DMPS) that was used to amplify 31 near-complete mitochondrial genomes of the extinct cave bear (Ursus spelaeus). DMPS couples multiplex PCR with the generation of barcoded sequencing libraries to be sequenced in parallel on a high-throughput sequencing platform. DMPS makes it possible to generate large amounts of targeted DNA sequence data simultaneously from multiple degraded samples such as fossil remains. In this chapter, I describe an experiment that uses DMPS with different primer sets and on both modern and ancient DNA templates.
authors.affiliation: Department of Biology, The Pennsylvania State University, 320 Mueller Laboratory, University Park, PA 16802, USA. mus52@psu.edu
authors.forename: Mathias
authors.initials: M
authors.lastname: Stiller
mesh_terms: D000818:Animals; D004272:DNA, Mitochondrial / Q000235:genetics*; D053476:Extinction, Biological; D005580:Fossils; D054629:Genome, Mitochondrial; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D001503:Ursidae / Q000235:genetics*
chemical_list: D004272:DNA, Mitochondrial