title: Whey protein ingestion activates mTOR-dependent signalling after resistance exercise in young men: a double-blinded randomized controlled trial.
abstract: The effect of resistance exercise with the ingestion of supplementary protein on the activation of the mTOR cascade, in human skeletal muscle has not been fully elucidated. In this study, the impact of a single bout of resistance exercise, immediately followed by a single dose of whey protein isolate (WPI) or placebo supplement, on the activation of mTOR signalling was analyzed. Young untrained men completed a maximal single-legged knee extension exercise bout and were randomized to ingest either WPI supplement (n = 7) or the placebo (n = 7). Muscle biopsies were taken from the vastus lateralis before, and 2, 4 and 24 h post-exercise. WPI or placebo ingestion consumed immediately post-exercise had no impact on the phosphorylation of Akt (Ser473). However, WPI significantly enhanced phosphorylation of mTOR (Ser2448), 4E-BP1 (Thr(37/46)) and p70(S6K) (Thr389) at 2 h post-exercise. This study demonstrates that a single dose of WPI, when consumed in modest quantities, taken immediately after resistance exercise elicits an acute and transient activation of translation initiation within the exercised skeletal muscle.
authors.affiliation: Molecular Nutrition Unit, Deakin University, Burwood, Victoria, 3125, Australia. Michelle.Farnfield@y7mail.com
authors.forename: Michelle M
authors.initials: MM
authors.lastname: Farnfield
authors.forename: Kate A
authors.initials: KA
authors.lastname: Carey
authors.forename: Petra
authors.initials: P
authors.lastname: Gran
authors.forename: Marissa K
authors.initials: MK
authors.lastname: Trenerry
authors.forename: David
authors.initials: D
authors.lastname: Cameron-Smith
mesh_terms: D000293:Adolescent; D000328:Adult; D019587:Dietary Supplements; D004311:Double-Blind Method; D015444:Exercise / Q000502:physiology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D008297:Male; D008894:Milk Proteins / Q000008:administration & dosage / Q000494:pharmacology*; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D058570:TOR Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors; D000067816:Whey Proteins; D055815:Young Adult
chemical_list: D008894:Milk Proteins; D000067816:Whey Proteins; C546842:MTOR protein, human; D058570:TOR Serine-Threonine Kinases