title: Identification and validation of sex-linked SCAR markers in dioecious Hippophae rhamnoides L. (Elaeagnaceae).
abstract: The actinorhizal plant seabuckthorn (Hippophae rhamnoides L., Elaeagnaceae) is a wind pollinated dioecious crop. To distinguish male genotypes from female genotypes early in the vegetative growth phase, we have developed robust PCR-based marker(s). DNA bulk samples from 20 male and 20 female plants each were screened with 60 RAPD primers. Two primers, OPA-04 and OPT-06 consistently amplified female-specific (FS) polymorphic fragments of 1,164 and 868 bp, respectively, that were absent in the male samples. DNA sequence of the two markers did not exhibit significant similarity to previously characterized sequences. A sequence-characterized amplified region marker HrX1 (JQ284019) and HrX2 (JQ284020) designed for the two fragments, continued to amplify the FS allele in 120 female plants but not in 100 male plants tested in the current study. Thus, HrX1 and HrX2 are FS markers that can determine the sex of seabuckthorn plants in an early stage and expedite cultivations for industrial applications.
authors.affiliation: Defence Institute of High Altitude Research, Defence R & D Organisation, Leh-Ladakh, 194101, India.
authors.forename: Girish
authors.initials: G
authors.lastname: Korekar
authors.forename: Ram Kumar
authors.initials: RK
authors.lastname: Sharma
authors.forename: Rahul
authors.initials: R
authors.lastname: Kumar
authors.lastname: Meenu
authors.forename: Naveen C
authors.initials: NC
authors.lastname: Bisht
authors.forename: Ravi B
authors.initials: RB
authors.lastname: Srivastava
authors.forename: Paramvir Singh
authors.initials: PS
authors.lastname: Ahuja
authors.forename: Tsering
authors.initials: T
authors.lastname: Stobdan
mesh_terms: D017931:DNA Primers / Q000235:genetics; D005838:Genotype; D031281:Hippophae / Q000235:genetics*; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D017422:Sequence Analysis, DNA; D012723:Sex
chemical_list: D017931:DNA Primers