title: Silk protein fibroin from Antheraea mylitta for cardiac tissue engineering.
abstract: The human heart cannot regenerate after an injury. Lost cardiomyocytes are replaced by scar tissue resulting in reduced cardiac function causing high morbidity and mortality. One possible solution to this problem is cardiac tissue engineering. Here, we have investigated the suitability of non-mulberry silk protein fibroin from Indian tropical tasar Antheraea mylitta as a scaffold for engineering a cardiac patch in vitro. We have tested cell adhesion, cellular metabolic activity, response to extracellular stimuli, cell-to-cell communication and contractility of 3-days postnatal rat cardiomyocytes on silk fibroin. Our data demonstrate that A. mylitta silk fibroin exhibits similar properties as fibronectin, a component of the natural matrix for cardiomyocytes. Comparison to mulberry Bombyx mori silk protein fibroin shows that A. mylitta silk fibroin is superior probably due to its RGD domains. 3D scaffolds can efficiently be loaded with cardiomyocytes resulting in contractile patches. In conclusion, our findings demonstrate that A. mylitta silk fibroin 3D scaffolds are suitable for the engineering of cardiac patches.
authors.affiliation: Department of Cardiac Development and Remodelling, Max-Planck-Institute for Heart and Lung Research, Parkstrasse 1, 61231 Bad Nauheim, Germany.
authors.forename: Chinmoy
authors.initials: C
authors.lastname: Patra
authors.forename: Sarmistha
authors.initials: S
authors.lastname: Talukdar
authors.forename: Tatyana
authors.initials: T
authors.lastname: Novoyatleva
authors.forename: Siva R
authors.initials: SR
authors.lastname: Velagala
authors.forename: Christian
authors.initials: C
authors.lastname: Mühlfeld
authors.forename: Banani
authors.initials: B
authors.lastname: Kundu
authors.forename: Subhas C
authors.initials: SC
authors.lastname: Kundu
authors.forename: Felix B
authors.initials: FB
authors.lastname: Engel
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002450:Cell Communication / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D005349:Fibroins / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects* / Q000502:physiology*; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D009036:Moths / Q000737:chemistry*; D032383:Myocytes, Cardiac / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009842:Oligopeptides / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid; D005349:Fibroins