title: Glycoproteomic characterization of recombinant mouse α-dystroglycan.
abstract: α-Dystroglycan (DG) is a key component of the dystrophin-glycoprotein complex. Aberrant glycosylation of the protein has been linked to various forms of congenital muscular dystrophy. Unusually α-DG has previously been demonstrated to be modified with both O-N-acetylgalactosamine and O-mannose initiated glycans. In the present study, Fc-tagged recombinant mouse α-DG was expressed and purified from human embryonic kidney 293T cells. α-DG glycopeptides were characterized by glycoproteomic strategies using both nano-liquid chromatography matrix-assisted laser desorption ionization and electrospray tandem mass spectrometry. A total of 14 different peptide sequences and 38 glycopeptides were identified which displayed heterogeneous O-glycosylation. These data provide new insights into the complex domain-specific O-glycosylation of α-DG.
authors.affiliation: Division of Molecular Biosciences, Faculty of Natural Sciences,  Imperial College London, London SW7 2AZ,  UK.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Harrison
authors.forename: Paul G
authors.initials: PG
authors.lastname: Hitchen
authors.forename: Maria
authors.initials: M
authors.lastname: Panico
authors.forename: Howard R
authors.initials: HR
authors.lastname: Morris
authors.forename: David
authors.initials: D
authors.lastname: Mekhaiel
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Pleass
authors.forename: Anne
authors.initials: A
authors.lastname: Dell
authors.forename: Jane E
authors.initials: JE
authors.lastname: Hewitt
authors.forename: Stuart M
authors.initials: SM
authors.lastname: Haslam
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002853:Chromatography, Liquid; D049030:Dystroglycans / Q000737:chemistry*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D040901:Proteomics; D011994:Recombinant Proteins / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D011994:Recombinant Proteins; D049030:Dystroglycans