title: Nanostructured, highly aligned poly(hydroxy butyrate) electrospun fibers for differentiation of skeletal and cardiac muscle cells.
abstract: The influence of novel nanostructured anisotropically electrospun poly(hydroxy butyrate) matrices on skeletal and cardiac muscle-like cell proliferation and differentiation was investigated, in comparison with isotropic and no-topographically cues-provided substrates. After the matrix characterization, in terms of surface SEM imaging and mechanical properties, cell differentiation on the different substrates was evaluated. Myogenin and F-actin staining at several differentiation time-points suggested that aligned nanofibers promote differentiation of both cell types. Moreover, quantitative parameters for each cell line are provided to clarify which aspects of the differentiation process are influenced by the different matrix topographies.
authors.affiliation: BioRobotics Institute, Scuola Superiore Sant’Anna, and with the Center for Micro-Bio-Robotics @ SSSA, Istituto Italiano di Tecnologia, 56127 Pontedera, PI, Italy. l.ricotti@sssup.it
authors.forename: Leonardo
authors.initials: L
authors.lastname: Ricotti
authors.forename: Alessandro
authors.initials: A
authors.lastname: Polini
authors.forename: Giada G
authors.initials: GG
authors.lastname: Genchi
authors.forename: Gianni
authors.initials: G
authors.lastname: Ciofani
authors.forename: Donata
authors.initials: D
authors.lastname: Iandolo
authors.forename: Virgilio
authors.initials: V
authors.lastname: Mattoli
authors.forename: Arianna
authors.initials: A
authors.lastname: Menciassi
authors.forename: Paolo
authors.initials: P
authors.lastname: Dario
authors.forename: Dario
authors.initials: D
authors.lastname: Pisignano
mesh_terms: D001842:Bone and Bones / Q000166:cytology / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects*; D006321:Heart / Q000187:drug effects*; D006801:Humans; D006885:Hydroxybutyrates / Q000737:chemistry / Q000494:pharmacology*; D049329:Nanostructures; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D006885:Hydroxybutyrates; D011108:Polymers