title: Promoting nerve cell functions on hydrogels grafted with poly(L-lysine).
abstract: We present a novel photopolymerizable poly(L-lysine) (PLL) and use it to modify polyethylene glycol diacrylate (PEGDA) hydrogels for creating a better, permissive nerve cell niche. Compared with their neutral counterparts, these PLL-grafted hydrogels greatly enhance pheochromocytoma (PC12) cell survival in encapsulation, proliferation, and neurite growth and also promote neural progenitor cell proliferation and differentiation capacity, represented by percentages of both differentiated neurons and astrocytes. The role of efficiently controlled substrate stiffness in regulating nerve cell behavior is also investigated and a polymerizable cationic small molecule, [2-(methacryloyloxy)ethyl]-trimethylammonium chloride (MTAC), is used to compare with this newly developed PLL. The results indicate that these PLL-grafted hydrogels are promising biomaterials for nerve repair and regeneration.
authors.affiliation: Department of Materials Science and Engineering, The University of Tennessee, Knoxville, Tennessee 37996, United States.
authors.forename: Lei
authors.initials: L
authors.lastname: Cai
authors.forename: Jie
authors.initials: J
authors.lastname: Lu
authors.forename: Volney
authors.initials: V
authors.lastname: Sheen
authors.forename: Shanfeng
authors.initials: S
authors.lastname: Wang
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D020100:Hydrogels; D008027:Light; D009682:Magnetic Resonance Spectroscopy; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D016716:PC12 Cells; D055668:Photochemical Processes; D011092:Polyethylene Glycols / Q000737:chemistry*; D011107:Polylysine / Q000138:chemical synthesis* / Q000494:pharmacology; D058105:Polymerization; D051381:Rats; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D020100:Hydrogels; C437167:poly(ethylene glycol)diacrylate; D011107:Polylysine; D011092:Polyethylene Glycols