title: Affinity-based proteomic profiling: problems and achievements.
abstract: Affinity chromatography becomes a more and more popular method used in proteomic studies for separation of various groups of proteins (subproteomes). The review highlights the role of affinity chromatography fractionation for proteomic profiling of the most of intensively studied groups of proteins including cyclic nucleotide-binding proteins, protein kinases (kinomes), phosphoproteins, glycoproteins, ubiquitinated proteins. Special attention is paid to the use of affinity chromatography for the characterization of small-molecule protein targets. The latter is especially important for the elucidation of direct protein targets of potential drug substances for evaluation of their possible side-effects or additional pharmacological application.
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russian Federation. professor57@yandex.ru.
authors.forename: Alexei
authors.initials: A
authors.lastname: Medvedev
authors.forename: Arthur
authors.initials: A
authors.lastname: Kopylov
authors.forename: Olga
authors.initials: O
authors.lastname: Buneeva
authors.forename: Victor
authors.initials: V
authors.lastname: Zgoda
authors.forename: Alexander
authors.initials: A
authors.lastname: Archakov
mesh_terms: D002458:Cell Fractionation; D005591:Chemical Fractionation; D002846:Chromatography, Affinity / Q000379:methods*; D020869:Gene Expression Profiling; D054730:Protein Interaction Domains and Motifs; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D040901:Proteomics / Q000379:methods*
chemical_list: D011506:Proteins