title: Development of a modified gentamicin resistance cassette for genetic manipulation of the oral spirochete Treponema denticola.
abstract: Herein, we report that a modified gentamicin cassette and a PCR-based method can be used for targeted mutagenesis of the oral spirochete Treponema denticola. This approach minimizes polar effects and spontaneous antibiotic resistance. Therefore, it can serve as a reliable tool for genetic manipulation of T. denticola.
authors.affiliation: Department of Oral Biology, State University of New York at Buffalo, Buffalo, New York, USA.
authors.forename: Jiang
authors.initials: J
authors.lastname: Bian
authors.forename: J Christopher
authors.initials: JC
authors.lastname: Fenno
authors.forename: Chunhao
authors.initials: C
authors.lastname: Li
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D005798:Genes, Bacterial; D005827:Genetics, Microbial / Q000379:methods*; D005839:Gentamicins / Q000494:pharmacology*; D016254:Mutagenesis, Insertional / Q000379:methods*; D012641:Selection, Genetic; D048373:Treponema denticola / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D005839:Gentamicins