title: [Effects of lipopolysaccharides from various Porphyromonas on the expression of CD14 and TLRs in mouse osteoblast].
abstract: OBJECTIVE
  To observe the effect of lipopolysaccharides(LPS) extracted from Porphyromonas endodontalis(P.e) and Porphyromonas gingivals(P.g) on the expression of CD14 and TLRs in osteoblast.
  
  
  METHODS
  MC3T3-E1 cells were stimulated with 10μg/mL P.e-LPS and P.g-LPS. The change of CD14,TLR2 and TLR4 mRNA was observed at different time point (0,1,3,6,12,24h) using RT-PCR,and the expression of CD14,TLR2 and TLR4 protein was measured by flow cytometry at 24-hour. Statistical analysis was performed using one-way ANOVA and Dunnett t test with SPSS11.0 software package.
  
  
  RESULTS
  MC3T3-E1 cells were stimulated with 10μg/mL P.e-LPS for 1h,the expression of CD14 and TLR4 mRNA increased significantly. There was no increase of TLR2 mRNA with stimulation of P.e-LPS. The CD14,TLR2 and TLR4 mRNA expression increased significantly after stimulation with 10μg/mL P.g-LPS. Flow cytometry showed that CD14 and TLR4 protein increased significantly after stimulation with 10μg/mL P.e-LPS. CD14,TLR2 and TLR4 protein increased significantly after treatment with 10μg/mL P.g-LPS.
  
  
  CONCLUSIONS
  CD14,TLR4 receptors are involved in P.e-LPS effect and CD14,TLR2 and TLR4 receptors are involved in P.g-LPS effect in mouse osteoblast.
authors.affiliation: Department of Endodontics, School of Stomatology, China Medical University, Stomatological Research Institute of Liaoning Province, Shenyang 110002, Liaoning Province, China. 1985jiage@163.com
authors.forename: Ge
authors.initials: G
authors.lastname: Jia
authors.forename: Ming
authors.initials: M
authors.lastname: Xue
authors.forename: Ren
authors.initials: R
authors.lastname: Li
authors.forename: You
authors.initials: Y
authors.lastname: Lv
authors.forename: Li-hong
authors.initials: LH
authors.lastname: Qiu
mesh_terms: D000818:Animals; D008070:Lipopolysaccharides; D051379:Mice; D010006:Osteoblasts; D017861:Porphyromonas; D012333:RNA, Messenger; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4
chemical_list: D008070:Lipopolysaccharides; D012333:RNA, Messenger; C495345:TLR4 protein, human; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4