title: Effect of ouabain on myocardial ultrastructure and cytoskeleton during the development of ventricular hypertrophy.
abstract: The aim of this work is to study  cytoskeletal impairment during the development of ouabain-induced ventricular hypertrophy. Male Sprague-Dawley rats were treated with either ouabain or saline. Systolic blood pressure (SBP) was recorded weekly. At the end of the 3rd and 6th week, the rats were killed and cardiac mass index were measured. Hematoxylin-eosin and Sirius red staining were carried out and cardiac ultrastructure were studied using transmission electron microscopy. The mRNA level of Profilin-1, Desmin, PCNA, TGF-β(1) and ET-1 in the left ventricle were measured using real-time quantitative PCR while their protein levels were examined by Western blot or immunohistochemistry. After 3 weeks, there was no significant difference in the mean SBP, cardiac mass index, mRNA and protein expression of PCNA, TGF-β(1) and ET-1 between the two groups. However, ouabain-treated rats showed disorganized cardiac cytoskeleton with abnormal expression of Profilin-1 and Desmin. After 6 weeks, the cardiac mass index remained the same in the two groups while PCNA, TGF-β(1), and ET-1 have been upregulated in ouabain-treated rats. The cardiac cytoskeletal impairment was more severe in ouabain-treated rats with further changes of Profilin-1 and Desmin. Cytoskeletal abnormality is an ultra-early change during ouabain-induced ventricular hypertrophy, before the release of hypertrophic factors. Therapy for prevention of ouabain-induced hypertrophy should start at the early stage by preventing the cytoskeleton from disorganization.
authors.affiliation: Department of Geriatric Cardiology, Qilu Hospital of Shandong University, 107 Wenhua Xi Road, Jinan, People's Republic of China.
authors.forename: Shao-hua
authors.initials: SH
authors.lastname: Zhao
authors.forename: Hai-qing
authors.initials: HQ
authors.lastname: Gao
authors.forename: Xiang
authors.initials: X
authors.lastname: Ji
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiang-ju
authors.initials: XJ
authors.lastname: Liu
authors.forename: Bei-an
authors.initials: BA
authors.lastname: You
authors.forename: Xiao-pei
authors.initials: XP
authors.lastname: Cui
authors.forename: Jie
authors.initials: J
authors.lastname: Qiu
mesh_terms: D000818:Animals; D001794:Blood Pressure; D003599:Cytoskeleton / Q000187:drug effects* / Q000648:ultrastructure; D003893:Desmin / Q000096:biosynthesis / Q000235:genetics; D004195:Disease Models, Animal; D018450:Disease Progression; D005786:Gene Expression Regulation / Q000187:drug effects; D017379:Hypertrophy, Left Ventricular / Q000139:chemically induced / Q000473:pathology* / Q000503:physiopathology; D008297:Male; D046529:Microscopy, Electron, Transmission; D009206:Myocardium / Q000378:metabolism / Q000648:ultrastructure*; D010042:Ouabain / Q000633:toxicity*; D051304:Profilins / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D003893:Desmin; D051304:Profilins; D012333:RNA, Messenger; D010042:Ouabain