title: Effects of Anemarrhena asphodeloides on IgE-mediated passive cutaneous anaphylaxis, compound 48/80-induced systemic anaphylaxis and mast cell activation.
abstract: BACKGROUND
  We investigated the effect of Anemarrhena asphodeloides Bunge (Liliaceae) water extract (AAWE) on mast cell-mediated anaphylactic reactions. Mast cell-mediated anaphylactic reaction is involved in many allergic diseases, including asthma and allergic rhinitis. In Korea, where it has been used as a traditional medicine, AAWE is known to have antioxidant and anticancer activity. However, its specific effect on mast cell-mediated anaphylactic reactions is still unknown.
  
  
  METHODS
  We examined whether or not AAWE could inhibit IgE-mediated passive cutaneous anaphylaxis (PCA), compound 48/80-induced systemic anaphylaxis, and mast cell activation.
  
  
  RESULTS
  Oral administration of AAWE inhibited compound 48/80-induced systemic anaphylaxis in mice. AAWE also inhibited the local allergic reaction, PCA, activated by anti-dinitrophenyl IgE antibody in rats. AAWE reduced compound 48/80-induced degranulation of rat peritoneal mast cells (RPMCs). Moreover, AAWE inhibited histamine release and calcium uptake of RPMCs induced by compound 48/80 in a dose-dependent manner. AAWE also significantly inhibited secretion of tumor necrosis factor-α, interleukin (IL)-1β, and IL-6 in phorbol 12-myristate 13-acetate plus calcium ionophores A23187-stimulated RPMCs.
  
  
  CONCLUSIONS
  These results suggest that AAWE suppresses compound 48/80-induced mast cell activation by inhibition of cellular mechanisms in signaling pathways, and would be beneficial for treatment of mast cell-mediated anaphylactic response.
authors.affiliation: Department of Anatomy, Chonbuk National University Medical School and Institute for Medical Sciences, Chonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si, Jeollabuk-do 561-756, Republic of Korea.
authors.forename: Ok Hee
authors.initials: OH
authors.lastname: Chai
authors.forename: Dong-Hwa
authors.initials: DH
authors.lastname: Shon
authors.forename: Eui-Hyeog
authors.initials: EH
authors.lastname: Han
authors.forename: Hyoung Tae
authors.initials: HT
authors.lastname: Kim
authors.forename: Chang Ho
authors.initials: CH
authors.lastname: Song
mesh_terms: D000707:Anaphylaxis / Q000276:immunology*; D031403:Anemarrhena / Q000737:chemistry; D000818:Animals; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006636:Histamine Release / Q000187:drug effects; D007073:Immunoglobulin E; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects*; D008517:Phytotherapy / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects; D003189:p-Methoxy-N-methylphenethylamine / Q000276:immunology / Q000494:pharmacology
chemical_list: D004365:Drugs, Chinese Herbal; D007073:Immunoglobulin E; D003189:p-Methoxy-N-methylphenethylamine