title: Identification of a novel nanoRNase in Bartonella.
abstract: In Escherichia coli, only one essential oligoribonuclease (Orn) can degrade oligoribonucleotides of five residues and shorter in length (nanoRNA). In Bacillus subtilis, NrnA and NrnB, which do not show any sequence similarity to Orn, have been identified as functional analogues of Orn. Sequence comparisons did not identify orn, nrnA or nrnB homologues in the genomes of the Chlamydia/Cyanobacteria and Alphaproteobacteria family members. Screening a genomic library from Bartonella birtlesii, a member of the Alphaproteobacteria, for genes that can complement a conditional orn mutant in E. coli, we identified BA0969 (NrnC) as a functional analogue of Orn. NrnC is highly conserved (more than 80 % identity) in the Bartonella genomes sequenced to date. Biochemical characterization showed that this protein exhibits oligo RNA degradation activity (nanoRNase activity). Like Orn from E. coli, NrnC is inhibited by micromolar amounts of 3'-phosphoadenosine 5'-phosphate in vitro. NrnC homologues are widely present in genomes of Alphaproteobacteria. Knock down of nrnC decreases the growth ability of Bartonella henselae, demonstrating the importance of nanoRNase activity in this bacterium.
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, Jilin University, 5333 Xi an Road, Changchun 130062, PR China.|UMR BIPAR INRA-AFSSA-ENVA, 23 Avenue du Général de Gaulle, 94700 Maisons-Alfort, France.
authors.forename: Ma Feng
authors.initials: MF
authors.lastname: Liu
authors.affiliation: Pasteur Institute, 25-28 Rue du Dr Roux, 75015 Paris, France.
authors.forename: Sandra
authors.initials: S
authors.lastname: Cescau
authors.affiliation: Pasteur Institute, 25-28 Rue du Dr Roux, 75015 Paris, France.
authors.forename: Undine
authors.initials: U
authors.lastname: Mechold
authors.affiliation: Key Lab of Mental Health, Institute of Psychology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel.
authors.forename: Dorit
authors.initials: D
authors.lastname: Cohen
authors.affiliation: AMAbiotics SAS, Bldg G1, 2 rue Gaston Crémieux, 91000 Evry, France.
authors.forename: Antoine
authors.initials: A
authors.lastname: Danchin
authors.affiliation: UMR BIPAR INRA-AFSSA-ENVA, 23 Avenue du Général de Gaulle, 94700 Maisons-Alfort, France.
authors.forename: Henri-Jean
authors.initials: HJ
authors.lastname: Boulouis
authors.affiliation: Pasteur Institute, 25-28 Rue du Dr Roux, 75015 Paris, France.|UMR BIPAR INRA-AFSSA-ENVA, 23 Avenue du Général de Gaulle, 94700 Maisons-Alfort, France.
authors.forename: Francis
authors.initials: F
authors.lastname: Biville
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001473:Bartonella / Q000201:enzymology / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005095:Exoribonucleases / Q000235:genetics / Q000378:metabolism*; D055785:Gene Knockdown Techniques; D005816:Genetic Complementation Test; D015698:Genomic Library; D008969:Molecular Sequence Data; D020871:RNA Stability; D012329:RNA, Bacterial / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D012329:RNA, Bacterial; D005095:Exoribonucleases; C094587:oligoribonuclease