title: [Anti-HIV activity and mechanism of Cynanchum otophyllum glucan sulfate in vitro].
abstract: OBJECTIVE
  To study anti-HIV activity and mechanism of Cynanchum otophyllum glucan sulfate in vitro.
  
  
  METHODS
  Anti-HIV-1 activity was detected with syncytial formation assay and quantitative P24 enzyme-linked immunosorbent assay (ELISA); cytotoxicity was tested with MTT colorimetric assay. Antiviral mechanism was investigated by fusion inhibition, time of addition and pre-treatment experiments.
  
  
  RESULTS
  The 50% inhibition concentrations (IC50) of PS20 for HIV-1(IIIB), HIV-1(Ada-M), and HIV-1(Bal), were 0.26, 0.46, 0.90 micromol x L(-1), respectively. Studies on antiviral mechanism of PS20 showed that target molecule may be viral envelope protein.
  
  
  CONCLUSIONS
  The results suggested that PS20 had high anti-HIV activity and was worth to be studied further.
authors.affiliation: Kunming University, Kunming 650214, China. kmtaojian@sina.com
authors.forename: Jian
authors.initials: J
authors.lastname: Tao
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Chaoyin
authors.initials: C
authors.lastname: Chen
authors.forename: Shenglan
authors.initials: S
authors.lastname: Zhao
authors.forename: Kunlong
authors.initials: K
authors.lastname: Ben
mesh_terms: D019380:Anti-HIV Agents / Q000494:pharmacology*; D002459:Cell Fusion; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D031105:Cynanchum / Q000737:chemistry*; D005936:Glucans / Q000302:isolation & purification* / Q000494:pharmacology*; D015497:HIV-1 / Q000187:drug effects*; D006801:Humans; D020128:Inhibitory Concentration 50; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry / Q000378:metabolism; D014759:Viral Envelope Proteins / Q000187:drug effects*
chemical_list: D019380:Anti-HIV Agents; D005936:Glucans; D010936:Plant Extracts; D014759:Viral Envelope Proteins; C078962:glucan sulfate