title: Serotonin-mediated modulation of Na+/K+ pump current in rat hippocampal CA1 pyramidal neurons.
abstract: BACKGROUND
  The aim of this study was to investigate whether serotonin (5-hydroxytryptamine, 5-HT) can modulate Na+/K+ pump in rat hippocampal CA1 pyramidal neurons.
  
  
  RESULTS
  5-HT (0.1, 1 mM) showed Na+/K+ pump current (Ip) densities of 0.40 ± 0.04, 0.34 ± 0.03 pA/pF contrast to 0.63 ± 0.04 pA/pF of the control of 0.5 mM strophanthidin (Str), demonstrating 5-HT-induced inhibition of Ip in a dose-dependent manner in hippocampal CA1 pyramidal neurons. The effect was partly attenuated by ondasetron, a 5-HT3 receptor (5-HT3R) antagonist, not by WAY100635, a 5-HT1AR antagonist, while 1-(3-Chlorophenyl) biguanide hydrochloride (m-CPBG), a 5-HT3R specific agonist, mimicked the effect of 5-HT on Ip.
  
  
  CONCLUSIONS
  5-HT inhibits neuronal Na+/K+ pump activity via 5-HT3R in rat hippocampal CA1 pyramidal neurons. This discloses novel mechanisms for the function of 5-HT in learning and memory, which may be a useful target to benefit these patients with cognitive disorder.
authors.affiliation: Department of Pharmacology, Hebei Medical University, Shijiazhuang, China.
authors.forename: Li Nan
authors.initials: LN
authors.lastname: Zhang
authors.forename: Su Wen
authors.initials: SW
authors.lastname: Su
authors.forename: Fang
authors.initials: F
authors.lastname: Guo
authors.forename: Hui Cai
authors.initials: HC
authors.lastname: Guo
authors.forename: Xiao Lu
authors.initials: XL
authors.lastname: Shi
authors.forename: Wen Ya
authors.initials: WY
authors.lastname: Li
authors.forename: Xu
authors.initials: X
authors.lastname: Liu
authors.forename: Yong Li
authors.initials: YL
authors.lastname: Wang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001645:Biguanides / Q000494:pharmacology; D001703:Biophysics; D056547:CA1 Region, Hippocampal / Q000166:cytology*; D004305:Dose-Response Relationship, Drug; D004558:Electric Stimulation; D066298:In Vitro Techniques; D009433:Neural Inhibition / Q000187:drug effects; D018408:Patch-Clamp Techniques; D010879:Piperazines / Q000494:pharmacology; D017966:Pyramidal Cells / Q000187:drug effects* / Q000502:physiology; D011725:Pyridines / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012701:Serotonin / Q000494:pharmacology*; D012702:Serotonin Antagonists / Q000494:pharmacology; D017366:Serotonin Receptor Agonists / Q000494:pharmacology; D026941:Sodium Channel Blockers / Q000494:pharmacology; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013327:Strophanthidin / Q000494:pharmacology; D013779:Tetrodotoxin / Q000494:pharmacology
chemical_list: D001645:Biguanides; D010879:Piperazines; D011725:Pyridines; D012702:Serotonin Antagonists; D017366:Serotonin Receptor Agonists; D026941:Sodium Channel Blockers; D012701:Serotonin; D013779:Tetrodotoxin; D013327:Strophanthidin; C090413:N-(2-(4-(2-methoxyphenyl)-1-piperazinyl)ethyl)-N-(2-pyridinyl)cyclohexanecarboxamide; C065734:1-(3-chlorophenyl)biguanide; D000254:Sodium-Potassium-Exchanging ATPase