title: Isolation of cyanophycin from tobacco and potato plants with constitutive plastidic cphATe gene expression.
abstract: A chimeric cyanophycin synthetase gene composed of the cphATe coding region from the cyanobacterium Thermosynechococcus elongatus BP-1, the constitutive 35S promoter and the plastid targeting sequence of the integral photosystem II protein PsbY was transferred to the tobacco variety Petit Havanna SRI and the commercial potato starch production variety Albatros. The resulting constitutive expression of cyanophycin synthetase leads to polymer contents in potato leaf chloroplasts of up to 35 mg/g dry weight and in tuber amyloplasts of up to 9 mg/g dry weight. Both transgenic tobacco and potato were used for the development of isolation methods applicable for large-scale extraction of the polymer. Two different procedures were developed which yielded polymer samples of 80 and 90% purity, respectively.
authors.affiliation: Department of Chemistry, University of Rostock, Albert-Einstein-Str. 3A, 18059 Rostock, Germany. katija.neubauer@catalysis.de
authors.forename: Katja
authors.initials: K
authors.lastname: Neubauer
authors.forename: Maja
authors.initials: M
authors.lastname: Hühns
authors.forename: Tina
authors.initials: T
authors.lastname: Hausmann
authors.forename: Friederike
authors.initials: F
authors.lastname: Klemke
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Lockau
authors.forename: Uwe
authors.initials: U
authors.lastname: Kahmann
authors.forename: Elfriede K
authors.initials: EK
authors.lastname: Pistorius
authors.forename: Udo
authors.initials: U
authors.lastname: Kragl
authors.forename: Inge
authors.initials: I
authors.lastname: Broer
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification*; D002736:Chloroplasts / Q000737:chemistry / Q000235:genetics; D000458:Cyanobacteria / Q000235:genetics; D010453:Peptide Synthases / Q000235:genetics*; D018515:Plant Leaves / Q000737:chemistry / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics; D011108:Polymers / Q000737:chemistry; D011198:Solanum tuberosum / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D011108:Polymers; C024250:cyanophycin; D010453:Peptide Synthases; C113257:cyanophycin synthase, bacteria