title: "Point" centromeres of Saccharomyces harbor single centromere-specific nucleosomes.
abstract: The "point" centromere of budding yeast is genetically defined by an ≈ 125-bp sequence. Recent fluorescence measurements of kinetochore clusters have suggested that this sequence specifies multiple centromere histone 3 (CenH3) nucleosomes. However, high-resolution mapping demonstrates that there is only one CenH3 nucleosome per centromere, providing biochemical confirmation of the point centromere model.
authors.affiliation: Basic Sciences Division, Howard Hughes Medical Institute, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109-1024, USA. steveh@fhcrc.org
authors.forename: Steven
authors.initials: S
authors.lastname: Henikoff
authors.forename: Jorja G
authors.initials: JG
authors.lastname: Henikoff
mesh_terms: D001483:Base Sequence; D002503:Centromere / Q000235:genetics*; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D005453:Fluorescence; D006657:Histones / Q000235:genetics; D008957:Models, Genetic; D009707:Nucleosomes / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics
chemical_list: C092746:CSE4 protein, S cerevisiae; D002868:Chromosomal Proteins, Non-Histone; D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D006657:Histones; D009707:Nucleosomes; D029701:Saccharomyces cerevisiae Proteins; C521684:Scm3 protein, S cerevisiae