title: Detection of tip-enhanced fluorescence from loop-mediated isothermal amplification of hepatitis B virus by two-photon microscopy.
abstract: Tip-enhanced fluorescence of localized DNA replication by loop-mediated isothermal amplification (LAMP) is a potential way to observe real-time biological reaction confined in nanometer scale. We successfully coated Bst polymerase on the apex (~100 nm) of an atomic force microscope (AFM) tip and performed localized LAMP reaction of hepatitis B virus (HBV). By using this tip-based reaction, the replicated HBV DNA can be directly imaged to be 400~500 nm spots by using two-photon excitation fluorescence microscopy.
authors.affiliation: Institute of Biomedical Electronics and Bioinformatics, National Taiwan University, Taiwan.
authors.forename: Shih-Chung
authors.initials: SC
authors.lastname: Wei
authors.forename: Tsung-Liang
authors.initials: TL
authors.lastname: Chuang
authors.forename: Hui-Hsin
authors.initials: HH
authors.lastname: Lu
authors.forename: Chia-Chen
authors.initials: CC
authors.lastname: Chang
authors.forename: Da-Shin
authors.initials: DS
authors.lastname: Wang
authors.forename: Kung-Bin
authors.initials: KB
authors.lastname: Sung
authors.forename: Chii-Wann
authors.initials: CW
authors.lastname: Lin
mesh_terms: D015342:DNA Probes / Q000235:genetics / Q000648:ultrastructure; D004279:DNA, Viral / Q000235:genetics* / Q000648:ultrastructure; D006515:Hepatitis B virus / Q000235:genetics* / Q000648:ultrastructure; D007089:Image Enhancement / Q000379:methods*; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*; D015336:Molecular Probe Techniques; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*
chemical_list: D015342:DNA Probes; D004279:DNA, Viral