title: Fluorescent coumarin thiols measure biological redox couples.
abstract: In this report we present a new chemical probe, 3-HTC, that can reversibly and ratiometrically measure the thiol-disulfide equilibrium of biological systems. 3-HTC is composed of a coumarin that has a thiolate directly conjugated to its extended aromatic π system while formation of a disulfide attenuates this conjugation. The fluorescence and absorption properties of 3-HTC are therefore very sensitive to the redox state of its thiol. 3-HTC reacts reversibly with thiols and disulfides enabling its use to measure dynamic GSH/GSSH ratios in vitro as well as to monitor the reversible redox status of whole cell lysates.
authors.affiliation: Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia 30032, USA.
authors.forename: Khalilah G
authors.initials: KG
authors.lastname: Reddie
authors.forename: William H
authors.initials: WH
authors.lastname: Humphries
authors.forename: Charlo P
authors.initials: CP
authors.lastname: Bain
authors.forename: Christine K
authors.initials: CK
authors.lastname: Payne
authors.forename: Melissa L
authors.initials: ML
authors.lastname: Kemp
authors.forename: Niren
authors.initials: N
authors.lastname: Murthy
mesh_terms: D003374:Coumarins / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry*; D006801:Humans; D019169:Jurkat Cells; D015394:Molecular Structure; D010084:Oxidation-Reduction; D013438:Sulfhydryl Compounds / Q000032:analysis / Q000737:chemistry*
chemical_list: D003374:Coumarins; D005456:Fluorescent Dyes; D013438:Sulfhydryl Compounds; C030123:coumarin