title: DNA extraction from keratin and chitin.
abstract: DNA extracted from keratinous and chitinous materials can be a useful source of genetic information. To effectively liberate the DNA from these materials, buffers containing relatively high levels of DTT, proteinase K, and detergent are recommended, followed by purification using either silica-column or organic methods.
authors.affiliation: Natural History Museum of Denmark, University of Copenhagen, Østervoldgade 5-7, 1350 Copenhagen, Denmark.
authors.forename: Paula F
authors.initials: PF
authors.lastname: Campos
authors.forename: Thomas M P
authors.initials: TM
authors.lastname: Gilbert
mesh_terms: D000818:Animals; D002686:Chitin / Q000737:chemistry*; D004247:DNA / Q000302:isolation & purification* / Q000378:metabolism; D004226:Dithioerythritol / Q000737:chemistry; D019286:Endopeptidase K / Q000378:metabolism; D005241:Feathers / Q000737:chemistry; D005580:Fossils; D006197:Hair / Q000737:chemistry; D007633:Keratins / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D002686:Chitin; D007633:Keratins; D004226:Dithioerythritol; D012822:Silicon Dioxide; D004247:DNA; D019286:Endopeptidase K