title: [Analysis of the changes of amide A in rabbit heart after death by FTIR spectroscopy].
abstract: OBJECTIVE
  To explore the relationship of changes of amide A in rabbit heart and the postmortem interval (PMI) by FTIR spectroscopy technique.
  
  
  METHODS
  Thirty-two rabbits were sacrificed and the hearts were sampled at 20 degrees C within 48 h postmortem points. All samples were sliced and tested by FTIR spectroscopy technique. The images of amide A were created by FTIR spectroscopic imaging. The positive and negative area ratios of amide A were analyzed using imaging analysis system.
  
  
  RESULTS
  The positive and negative area ratios declined regularly with the prolongation of death time in 48 h. There was a significant quadric relationship between the area ratios (y) of amide A (positive and negative area) and PMI(x). The regression equation was y = 0.001x2-0.038x + 0.747(R2 = 0.940).
  
  
  CONCLUSIONS
  The ratios of positive and negative area of amide A showed a strong correlation with PMI and could be used to estimate PMI.
authors.affiliation: Key Laboratory of Evidence Science, China University of Political Science and Law, Ministry of Education, Beijing 100040, China. zhd_zzw@126.com
authors.forename: Hai-Dong
authors.initials: HD
authors.lastname: Zhang
authors.forename: Tian-Tong
authors.initials: TT
authors.lastname: Yang
authors.forename: Na
authors.initials: N
authors.lastname: Zheng
authors.forename: Ting-Yi
authors.initials: TY
authors.lastname: Sun
authors.forename: Liang
authors.initials: L
authors.lastname: Liu
mesh_terms: D000577:Amides / Q000378:metabolism*; D000818:Animals; D005260:Female; D049429:Forensic Pathology / Q000379:methods; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D011180:Postmortem Changes; D011817:Rabbits; D012044:Regression Analysis; D017550:Spectroscopy, Fourier Transform Infrared; D013997:Time Factors
chemical_list: D000577:Amides