title: Phenolic composition and antioxidant properties of Polish blue-berried honeysuckle genotypes by HPLC-DAD-MS, HPLC postcolumn derivatization with ABTS or FC, and TLC with DPPH visualization.
abstract: In this study, different Polish cultivars of blue-berried honeysuckles (Lonicera caerulea L.), wild and bog bilberry, were analyzed for bioactive compounds. The chemical properties verified included composition of anthocyanins and other polyphenols, antioxidant activity, and profiles of antioxidants by HPLC postcolumn derivatization or TLC. The antioxidant activities of different blue-berried honeysuckle cultivars were similar to that of wild-growing bilberries (ranging from 170 to 417 μmol TE/g dm in ABTS and from 93 to 166 μmol TE/g dm in DPPH and Folin-Ciocalteu tests). The major anthocyanin in the blue-berried honeysuckle was cyanidin-3-glucoside, which constituted 84-92% of the total anthocyanins. The TLC and HPLC postcolumn antioxidant profiles indicated that anthocyanins are the major antioxidants in all berries studied. Wild berries and the cultivars of the blue-berried honeysuckles are also a similar source of such minerals as K, Mg, and Ca.
authors.affiliation: Department of Food Chemistry, Technology and Biotechnology, Chemical Faculty, Gdansk University of Technology, Gdansk, Poland. barbara.kusznierewicz@pg.gda.pl
authors.forename: Barbara
authors.initials: B
authors.lastname: Kusznierewicz
authors.forename: Anna
authors.initials: A
authors.lastname: Piekarska
authors.forename: Barbara
authors.initials: B
authors.lastname: Mrugalska
authors.forename: Piotr
authors.initials: P
authors.lastname: Konieczka
authors.forename: Jacek
authors.initials: J
authors.lastname: Namieśnik
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Bartoszek
mesh_terms: D000872:Anthocyanins / Q000032:analysis; D000975:Antioxidants / Q000032:analysis* / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer / Q000379:methods*; D005638:Fruit / Q000737:chemistry*; D005838:Genotype; D031239:Lonicera / Q000737:chemistry* / Q000235:genetics; D010636:Phenols / Q000032:analysis*; D011044:Poland; D059808:Polyphenols / Q000032:analysis; D029800:Vaccinium myrtillus / Q000737:chemistry
chemical_list: D000872:Anthocyanins; D000975:Antioxidants; D010636:Phenols; D059808:Polyphenols