title: Use of focussed beam reflectance measurement (FBRM) for monitoring changes in biomass concentration.
abstract: The potential of focussed beam reflectance measurement (FBRM) as a tool to monitor changes in biomass concentration was investigated in a number of biological systems. The measurement technique was applied to two morphologically dissimilar plant cell suspension cultures, Morinda citrifolia and Centaurea calcitrapa, to a filamentous bacteria, Streptomyces natalensis, to high density cultures of Escherichia coli and to a murine Sp2/0 hybridoma suspension cell line, 3-2.19. In all cases, the biomass concentration proved to be correlated with total FBRM counts. The nature of the correlation varied between systems and was influenced by the concentration, nature, size and morphology of the particle under investigation.
authors.affiliation: UCD School of Chemical and Bioprocess Engineering, UCD, Belfield, Dublin 4, Ireland.
authors.forename: Jessica
authors.initials: J
authors.lastname: Whelan
authors.forename: Eilis
authors.initials: E
authors.lastname: Murphy
authors.forename: Alan
authors.initials: A
authors.lastname: Pearson
authors.forename: Paul
authors.initials: P
authors.lastname: Jeffers
authors.forename: Patricia
authors.initials: P
authors.lastname: Kieran
authors.forename: Susan
authors.initials: S
authors.lastname: McDonnell
authors.forename: Sara
authors.initials: S
authors.lastname: Raposo
authors.forename: Ma Emília
authors.initials: ME
authors.lastname: Lima-Costa
authors.forename: Brian
authors.initials: B
authors.lastname: Glennon
mesh_terms: D000818:Animals; D018533:Biomass; D019149:Bioreactors; D031179:Centaurea / Q000166:cytology; D004926:Escherichia coli / Q000166:cytology / Q000254:growth & development; D006825:Hybridomas / Q000166:cytology; D051379:Mice; D032066:Morinda / Q000166:cytology; D059828:Plant Cells; D013302:Streptomyces / Q000166:cytology / Q000254:growth & development