title: A coordination polymer nanobelt (CPNB)-based aptasensor for sulfadimethoxine.
abstract: A polymer-based aptasensor, which consisted of fluorescein amidite (FAM)-modified aptamers and coordination polymer nanobelts (CPNBs), was developed utilizing the fluorescence quenching effect to detect sulfadimethoxine residue in food products. A single-stranded DNA (ssDNA) aptamer, which was a specific bio-probe for sulfadimethoxine (Su13; 5'-GAGGGCAACGAGTGTTTATAGA-3'), was discovered by a magnetic bead-based systematic evolution of ligands by exponential enrichment (SELEX) technique, and the fluorescent quenchers CPNBs were produced by mixing AgNO(3) and 4,4'-bipyridine. This aptasensor easily and sensitively detected sulfadimethoxine in solution with a limit of detection (LOD) of 10ng/mL. Furthermore, the antibiotic dissolved in milk was also effectively detected with the same LOD value. In addition, this aptamer probe offered high specificity for sulfadimethoxine compared to other antibiotics. These valuable results provide ample evidence that the CPNB-based aptasensor can be used to quantify sulfadimethoxine residue in food products.
authors.affiliation: Department of Chemistry, Pohang University of Science and Technology, San 31, Hyoja-dong, Pohang, Gyungbuk 790-784, South Korea.
authors.forename: Kyung-Mi
authors.initials: KM
authors.lastname: Song
authors.forename: Euiyoung
authors.initials: E
authors.lastname: Jeong
authors.forename: Weejeong
authors.initials: W
authors.lastname: Jeon
authors.forename: Hunho
authors.initials: H
authors.lastname: Jo
authors.forename: Changill
authors.initials: C
authors.lastname: Ban
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis*; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D005453:Fluorescence; D057230:Limit of Detection; D008892:Milk / Q000737:chemistry; D008969:Molecular Sequence Data; D052156:SELEX Aptamer Technique; D013412:Sulfadimethoxine / Q000032:analysis*
chemical_list: D000890:Anti-Infective Agents; D013412:Sulfadimethoxine