title: Quantitative analysis of the lipidomes of the influenza virus envelope and MDCK cell apical membrane.
abstract: The influenza virus (IFV) acquires its envelope by budding from host cell plasma membranes. Using quantitative shotgun mass spectrometry, we determined the lipidomes of the host Madin-Darby canine kidney cell, its apical membrane, and the IFV budding from it. We found the apical membrane to be enriched in sphingolipids (SPs) and cholesterol, whereas glycerophospholipids were reduced, and storage lipids were depleted compared with the whole-cell membranes. The virus membrane exhibited a further enrichment of SPs and cholesterol compared with the donor membrane at the expense of phosphatidylcholines. Our data are consistent with and extend existing models of membrane raft-based biogenesis of the apical membrane and IFV envelope.
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany.
authors.forename: Mathias J
authors.initials: MJ
authors.lastname: Gerl
authors.forename: Julio L
authors.initials: JL
authors.lastname: Sampaio
authors.forename: Severino
authors.initials: S
authors.lastname: Urban
authors.forename: Lucie
authors.initials: L
authors.lastname: Kalvodova
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Verbavatz
authors.forename: Beth
authors.initials: B
authors.lastname: Binnington
authors.forename: Dirk
authors.initials: D
authors.lastname: Lindemann
authors.forename: Clifford A
authors.initials: CA
authors.lastname: Lingwood
authors.forename: Andrej
authors.initials: A
authors.lastname: Shevchenko
authors.forename: Cornelia
authors.initials: C
authors.lastname: Schroeder
authors.forename: Kai
authors.initials: K
authors.lastname: Simons
mesh_terms: D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry*; D002784:Cholesterol / Q000032:analysis; D004285:Dogs; D013058:Mass Spectrometry; D008563:Membrane Lipids / Q000032:analysis*; D009975:Orthomyxoviridae / Q000737:chemistry*; D013107:Sphingolipids / Q000032:analysis
chemical_list: D008563:Membrane Lipids; D013107:Sphingolipids; C050629:viral envelope lipids; D002784:Cholesterol