title: [Effects of adipose-derived stem cells-hyaluronic acid composite on healing of wound combined with radiation injury].
abstract: OBJECTIVE
  To observe and explore the effects of adipose-derived stem cells (ADSCs)-hyaluronic acid (HA) composite on healing of wound combined with radiation injury.
  
  
  METHODS
  The ADSCs were harvested from the fat tissue in groin of 10 inbred Sprague Dawley (SD) rats and were isolated and cultured by enzyme digestion. The ADSCs-HA composite was prepared with ADSCs (5 x 10(6) cells/mL) at passage 6 and HA (10 mg/mL). Thirty inbred SD rats, 15 males and 15 females, were randomly divided into groups A (n = 10), B (n = 10), and C (n = 10). A 2 cm x 2 cm full-thickness skin defect was made on the rat back before 20 Gy 60Co radiation exposure. One week after debridement, wounds were treated by petrolatum gauze in group A as the control group, by HA (0.4 mL) and petrolatum gauze in group B, and by ADSCs-HA composite (0.4 mL) and petrolatum gauze in group C. The microvessel density (MVD) and the distribution of CD90 positive cells were observed at 1st, 2nd, 3rd, and 4th weeks.
  
  
  RESULTS
  The wound healing was slower, and wound did not heal at 4th week and still filled with granulation tissue in group A; the wound healing of group B was faster than that of group A, and the wound did not heal completely with depression in the center at 4th week; the wound healed completely with epidermilizated surface and no obvious depression at 4th week in group C. The histological observation showed that MVD was significantly higher in group C than in groups A and B at the 1st, 2nd, and 3rd weeks (P < 0.05), and in group B than in group A at the 3rd week (P < 0.05); MVD was significantly higher in groups B and C than in group A (P < 0.05), but no significant difference was found between groups B and C (P > 0.05) at 4th week. No CD90 positive cell was found in groups A and B; CD 90 positive cells were observed in group C and gradually decreased with time.
  
  
  CONCLUSIONS
  ADSCs-HA composite can accelerate healing of wound combined with radiation injury by promoting and controlling wound angiogenesis.
authors.affiliation: Department of Plastic Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Yang
authors.forename: Xin
authors.initials: X
authors.lastname: Xing
authors.forename: Dayuan
authors.initials: D
authors.lastname: Xu
authors.forename: Jianguo
authors.initials: J
authors.lastname: Xu
authors.forename: Junhui
authors.initials: J
authors.lastname: Li
mesh_terms: D017667:Adipocytes / Q000166:cytology; D043925:Angiogenesis Inducing Agents; D000818:Animals; D005260:Female; D006820:Hyaluronic Acid / Q000627:therapeutic use*; D008297:Male; D011832:Radiation Injuries / Q000628:therapy*; D051381:Rats; D017207:Rats, Sprague-Dawley; D033581:Stem Cell Transplantation; D014945:Wound Healing
chemical_list: D043925:Angiogenesis Inducing Agents; D006820:Hyaluronic Acid