title: Effect of lanthanum on rooting of in vitro regenerated shoots of Saussurea involucrata Kar. et Kir.
abstract: In present study, the effect of lanthanum (La) on the rooting of regenerated shoots of Saussurea involucrata Kar. et Kir was analyzed. Rooting occurred from regenerated shoots inoculated on a medium supplemented with La, the plant rooting hormone indole-3-acetic acid (IAA), or both La and IAA together. The highest rooting efficiency (96%), root number/shoot (8.5), and root length (63 mm) were recorded in shoots cultured on medium containing 2.5 μM IAA combined with 100 μM La(3+). In order to elucidate the mechanism of rooting enhancement by La, we examined dynamic changes in antioxidant enzyme activities in plant tissue over time in culture. We found that the activities of peroxidase (POX) and superoxide dismutase (SOD) were significantly higher in plant tissue cultured in IAA plus La than in La or IAA alone. At the same time, the highest H(2)O(2) content was detected in plant tissue in the presence of 2.5 μM IAA plus 100 μM La(3+). In light of these data and previous results, we speculate that La enhanced IAA-induced rooting by acting as a mild abiotic stress to stimulate POX and SOD activities in plant cells. Then, IAA reacted with oxygen and POX to form the ternary complex enzyme-IAA-O(2) that dissociated into IAA radicals and O(2)(-). Subsequently, IAA-induced O(2)(-) readily converted to hydroxyl radical (HO·) via SOD-catalyzed dismutation. Finally, cell wall loosening and cell elongation occurred as a consequence of HO-dependent scission of wall components, leading to root growth. The treatment of IAA combined with La resulted in the highest plantlet survival (80%) compared to single treatments with IAA or La alone. These data suggest that rare earth elements enhance root morphogenesis and the growth of S. involucrata.
authors.affiliation: Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, School of Life Science, Northwest University, Xi'an, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Guo
authors.forename: Ling-Ling
authors.initials: LL
authors.lastname: Xu
authors.forename: Zhen-Jun
authors.initials: ZJ
authors.lastname: Guan
authors.forename: Ya-Hui
authors.initials: YH
authors.lastname: Wei
mesh_terms: D060387:Ascorbate Peroxidases / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D006861:Hydrogen Peroxide / Q000378:metabolism; D007210:Indoleacetic Acids / Q000494:pharmacology; D007811:Lanthanum / Q000494:pharmacology*; D010937:Plant Growth Regulators / Q000494:pharmacology; D018517:Plant Roots / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D018520:Plant Shoots / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D012038:Regeneration / Q000187:drug effects* / Q000502:physiology; D030441:Saussurea / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D013482:Superoxide Dismutase / Q000378:metabolism; D046509:Tissue Culture Techniques
chemical_list: D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D007811:Lanthanum; C030737:indoleacetic acid; D006861:Hydrogen Peroxide; D060387:Ascorbate Peroxidases; D002374:Catalase; D013482:Superoxide Dismutase