title: Convergent evolution led ribosome inactivating proteins to interact with ribosomal stalk.
abstract: Ribosome-inactivating proteins (RIPs) inhibit protein synthesis by depurinating an adenine on the sarcin-ricin loop (SRL) of the large subunit ribosomal RNA. Several RIPs interact with the C-terminal end of ribosomal stalk P proteins, and this interaction is required for their full activity. In contrast, the activity of Pokeweed Antiviral Protein is not affected by blocking this stalk component. Here, we provide evidence from phylogenetic analyses and sequence alignments suggesting that the interaction with the C-terminal end of P proteins evolved independently in different RIPs by convergent evolution.
authors.affiliation: Área de Biología Molecular, Departamento de Bioquímica y Ciencias Biológicas, UNSL and Instituto Multidisciplinario de Investigaciones Biológicas de San Luis (IMIBIO-SL-CONICET), San Luis, Argentina.
authors.forename: Walter J
authors.initials: WJ
authors.lastname: Lapadula
authors.forename: M Virginia
authors.initials: MV
authors.lastname: Sanchez-Puerta
authors.forename: Maximiliano Juri
authors.initials: MJ
authors.lastname: Ayub
mesh_terms: D000595:Amino Acid Sequence; D001499:Bayes Theorem; D004268:DNA-Binding Proteins / Q000378:metabolism; D019143:Evolution, Molecular; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D014176:Protein Biosynthesis; D012335:RNA, Ribosomal / Q000235:genetics / Q000378:metabolism; D054788:Ribosome Inactivating Proteins / Q000378:metabolism*; D012270:Ribosomes / Q000235:genetics* / Q000378:metabolism*; D012276:Ricin / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D004268:DNA-Binding Proteins; D012335:RNA, Ribosomal; D012276:Ricin; D054788:Ribosome Inactivating Proteins