title: Trypanosoma cruzi trypomastigotes induce cytoskeleton modifications during HeLa cell invasion.
abstract: It has been recently shown that Trypanosoma cruzi trypomastigotes subvert a constitutive membrane repair mechanism to invade HeLa cells. Using a membrane extraction protocol and high-resolution microscopy, the HeLa cytoskeleton and T. cruzi parasites were imaged during the invasion process after 15 min and 45 min. Parasites were initially found under cells and were later observed in the cytoplasm. At later stages, parasite-driven protrusions with parallel filaments were observed, with trypomastigotes at their tips. We conclude that T. cruzi trypomastigotes induce deformations of the cortical actin cytoskeleton shortly after invasion, leading to the formation of pseudopod-like structures.
authors.affiliation: Department of Cell Biology and Genetics, University of Maryland, College Park, MD, USA.
authors.forename: Maria Cecília
authors.initials: MC
authors.lastname: Fernandes
authors.forename: Leonardo Rodrigues de
authors.initials: LR
authors.lastname: Andrade
authors.forename: Norma Windsor
authors.initials: NW
authors.lastname: Andrews
authors.forename: Renato Arruda
authors.initials: RA
authors.lastname: Mortara
mesh_terms: D002462:Cell Membrane / Q000469:parasitology* / Q000648:ultrastructure; D003599:Cytoskeleton / Q000469:parasitology* / Q000648:ultrastructure; D006367:HeLa Cells / Q000469:parasitology / Q000648:ultrastructure; D006801:Humans; D013997:Time Factors; D014349:Trypanosoma cruzi / Q000502:physiology*