title: Concerted effort of centrosomal and Golgi-derived microtubules is required for proper Golgi complex assembly but not for maintenance.
abstract: Assembly of an integral Golgi complex is driven by microtubule (MT)-dependent transport. Conversely, the Golgi itself functions as an unconventional MT-organizing center (MTOC). This raises the question of whether Golgi assembly requires centrosomal MTs or can be self-organized, relying on its own MTOC activity. The computational model presented here predicts that each MT population is capable of gathering Golgi stacks but not of establishing Golgi complex integrity or polarity. In contrast, the concerted effort of two MT populations would assemble an integral, polarized Golgi complex. Indeed, while laser ablation of the centrosome did not alter already-formed Golgi complexes, acentrosomal cells fail to reassemble an integral complex upon nocodazole washout. Moreover, polarity of post-Golgi trafficking was compromised under these conditions, leading to strong deficiency in polarized cell migration. Our data indicate that centrosomal MTs complement Golgi self-organization for proper Golgi assembly and motile-cell polarization.
authors.affiliation: Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Vinogradova
authors.forename: Raja
authors.initials: R
authors.lastname: Paul
authors.forename: Ashley D
authors.initials: AD
authors.lastname: Grimaldi
authors.forename: Jadranka
authors.initials: J
authors.lastname: Loncarek
authors.forename: Paul M
authors.initials: PM
authors.lastname: Miller
authors.forename: Dmitry
authors.initials: D
authors.lastname: Yampolsky
authors.forename: Valentin
authors.initials: V
authors.lastname: Magidson
authors.forename: Alexey
authors.initials: A
authors.lastname: Khodjakov
authors.forename: Alex
authors.initials: A
authors.lastname: Mogilner
authors.forename: Irina
authors.initials: I
authors.lastname: Kaverina
mesh_terms: D002460:Cell Line; D002465:Cell Movement; D016764:Cell Polarity; D018385:Centrosome / Q000378:metabolism*; D003198:Computer Simulation; D006056:Golgi Apparatus / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D008870:Microtubules / Q000378:metabolism*; D015739:Nocodazole / Q000494:pharmacology
chemical_list: D015739:Nocodazole