title: [Significance of heparanase, bFGF and VEGF in angiogenesis for nasopharyngeal angiofibroma outspreading].
abstract: OBJECTIVE
  To observe the expression of HPA, bFGF and VEGF in nasopharyngeal angiofibroma, and then explore its significance of inducing angiogenesis in the tumor's expansibility growth.
  
  
  METHODS
  The expression of heparanase, bFGF, VEGF and CD105 were examined in 30 (I - II period 9 cases, III - IV period 21 cases) samples from nasopharyngeal angiofibroma and 20 inferior turbinate tissues by immunohistochemical staining technique. The microvascular density (MVD) were measured by the immunohistostaining of CD105. The MVD was analyzed with the clinical stage.
  
  
  RESULTS
  The positive rates of the HPA, bFGF and VEGF expression in JNA tissues were significantly higher than that in inferior turbinate group (P < 0.05). The positive rates of HPA, bFGF and VEGF expression in III - IV period were obviously higher than that in I - II period (P < 0.05). The expression of bFGF and VEGF in JNA tissues was respectively positive correlated with the HPA (r = by 0.499, 0.582, P < 0.05); In JNA tissues, the mean MVD in both HPA and bFGF positive group was higher than each one single positive group or both negative express group (P < 0.05). And the mean MVD in both HPA and VEGF positive group was higher than each one single positive group or both negative express group (P < 0.05).
  
  
  CONCLUSIONS
  HPA can induce angiogenesis to promote tumor growth by releasing bFGF and VEGF. Targeting the HPA can be a new direction in JNA adjuvant treatment.
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, First Affiliated Hospital of Fujian Medical University, Fuzhou 350005, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Zixiang
authors.initials: Z
authors.lastname: Yi
authors.forename: Dean
authors.initials: D
authors.lastname: Zhao
authors.forename: Miaoan
authors.initials: M
authors.lastname: Chen
authors.forename: Zhichun
authors.initials: Z
authors.lastname: Li
mesh_terms: D000293:Adolescent; D000328:Adult; D018322:Angiofibroma / Q000098:blood supply* / Q000473:pathology; D043925:Angiogenesis Inducing Agents; D002648:Child; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D005966:Glucuronidase / Q000378:metabolism*; D006801:Humans; D008297:Male; D009303:Nasopharyngeal Neoplasms / Q000098:blood supply* / Q000473:pathology; D009389:Neovascularization, Pathologic; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*; D055815:Young Adult; D000099175:Heparanase
chemical_list: D043925:Angiogenesis Inducing Agents; D016222:Fibroblast Growth Factor 2; D005966:Glucuronidase; D042461:Vascular Endothelial Growth Factor A; D000099175:Heparanase; C467484:VEGFA protein, human