title: Hormonal activities of new brominated flame retardants.
abstract: After the phase-out of two commercial mixtures of brominated flame retardants, an increasing number of alternative flame retardants have been introduced in commercial applications. None of them, however, has been thoroughly tested for its hormonal activity. We used two yeast reporter-gene assays to determine the potential of eleven compounds to interfere with estrogenic and androgenic pathways. Our data demonstrate the ability of 2,4,6-tribromophenol to lower the transcriptional activity of human estrogen and androgen receptors. A nominal IC(50) value of 14.1 μM for anti-estrogenic and 3.9 μM for anti-androgenic activity was obtained using the luciferase reporter. An IC(50) value of 9.2 μM was calculated for the anti-estrogenic activity measured by the β-galactosidase assay. Of the tested chemicals, this study highlights the endocrine disrupting effects of 2,4,6-tribromophenol whose occurrence in the environment should be monitored.
authors.affiliation: Laboratory of Environmental Biotechnology, Institute of Microbiology ASCR, v.v.i., Vídeňská 1083, 142 20 Prague, Czech Republic.
authors.forename: M
authors.initials: M
authors.lastname: Ezechiáš
authors.forename: K
authors.initials: K
authors.lastname: Svobodová
authors.forename: T
authors.initials: T
authors.lastname: Cajthaml
mesh_terms: D000726:Androgen Antagonists / Q000633:toxicity; D000728:Androgens / Q000378:metabolism*; D052244:Endocrine Disruptors / Q000633:toxicity*; D004965:Estrogen Antagonists / Q000633:toxicity; D004967:Estrogens / Q000378:metabolism*; D005411:Flame Retardants / Q000633:toxicity*; D006801:Humans; D006842:Hydrocarbons, Brominated / Q000633:toxicity*; D011944:Receptors, Androgen / Q000378:metabolism
chemical_list: D000726:Androgen Antagonists; D000728:Androgens; D052244:Endocrine Disruptors; D004965:Estrogen Antagonists; D004967:Estrogens; D005411:Flame Retardants; D006842:Hydrocarbons, Brominated; D011944:Receptors, Androgen