title: Enhanced co-production of S-adenosylmethionine and glutathione by an ATP-oriented amino acid addition strategy.
abstract: Effects of amino acids on co-production of S-adenosylmethionine (SAM) and glutathione (GSH) with Candida utilis CCTCC M 209298 were investigated. Both methionine and cysteine showed positive effects on improving intracellular SAM and GSH content simultaneously. Batch fermentative co-production of SAM and GSH was conducted in a 5L stirred fermentor, where the co-production of SAM and GSH was enhanced by 71.3% and 71.5% with 6g/L methionine and 6 mmol/L cysteine, respectively. Based on these results together with intracellular ATP levels during batch cultivation of C. utilis CCTCC M 209298, a two-stage amino acid addition strategy was developed by adding 6g/L methionine at 0 h and 6 mmol/L cysteine at 21 h. Under this ATP-oriented strategy, SAM and GSH biosynthesis was further promoted, and the co-production of SAM and GSH reached 669.3mg/L, which was increased by 124.6% compared to that without amino acid addition.
authors.affiliation: School of Biology and Basic Medical Sciences, Soochow University, Suzhou 215123, China.
authors.forename: Yulei
authors.initials: Y
authors.lastname: Wang
authors.forename: Dahui
authors.initials: D
authors.lastname: Wang
authors.forename: Gongyuan
authors.initials: G
authors.lastname: Wei
authors.forename: Na
authors.initials: N
authors.lastname: Shao
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000596:Amino Acids / Q000378:metabolism*; D002175:Candida / Q000378:metabolism*; D005978:Glutathione / Q000096:biosynthesis*; D007700:Kinetics; D012436:S-Adenosylmethionine / Q000096:biosynthesis*
chemical_list: D000596:Amino Acids; D012436:S-Adenosylmethionine; D000255:Adenosine Triphosphate; D005978:Glutathione