title: Endothelial cell adhesion and proliferation to PEGylated polymers with covalently linked RGD peptides.
abstract: A nonfouling peptide grafted polymer was synthesized that can promote endothelial cell (EC) binding. The polymer was composed of hexyl methacrylate, methyl methacrylate, poly(ethylene glycol) methacrylate, and CGRGDS peptide. The peptide was incorporated into the polymer system either by a chain transfer reaction or by coupling to an acrylate-PEG-N-hydroxysuccinimide (NHS) comonomer. The introduction of PEG chains minimizes protein adsorption. Human umbilical vein ECs and endothelial colony forming cells were cultured on these surfaces in short term and long-term studies. A difference in number and morphology of ECs was observed depending on the method of peptide incorporation. Both cell types adhered better to polymer films containing NHS coupled RGD peptide after 2 h even in the presence of albumin but significant cell detachment occurred after 4 days. Polymer solutions were electrospun into fibrous scaffolds. Both nonfouling and peptide binding characteristics were retained after processing.
authors.affiliation: Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, Ohio 43210, USA.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Daniel E
authors.initials: DE
authors.lastname: Heath
authors.forename: Stuart L
authors.initials: SL
authors.lastname: Cooper
mesh_terms: D002448:Cell Adhesion / Q000502:physiology*; D049109:Cell Proliferation; D020099:Coated Materials, Biocompatible; D042783:Endothelial Cells / Q000166:cytology / Q000502:physiology*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008422:Materials Testing; D015394:Molecular Structure; D009842:Oligopeptides / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D020099:Coated Materials, Biocompatible; D009842:Oligopeptides; D011108:Polymers; D011092:Polyethylene Glycols; C047981:arginyl-glycyl-aspartic acid