title: Peptide-mediated nanoengineering of inorganic particle surfaces: a general route toward surface functionalization via peptide adhesion domains.
abstract: The peptide-mediated functionalization of inorganic particle surfaces is demonstrated on gadolinium oxide (GdO) particles, revealing specific means to functionalize nano- or microparticles. Phage display screening is exploited to select 12mer peptides, which exhibit sequence-specific adhesion onto surfaces of GdO particles. These peptide adhesion domains are exploited to effectively decorate GdO particles with fluorescently labeled poly(ethylene oxide) (PEO), proving to result in a stable surface modification as shown by significant reduction of protein adsorption by 80%, compared to nonfunctionalized particles. Peptide adhesion and stability of the noncovalent coating are investigated by adsorption/elution experiments and Langmuir isotherms. Fluorescence microscopy, contact angle, and energy dispersive X-ray (EDX) measurements confirmed the sequence specificity of the interactions by comparing adhesion sequences with scrambled peptide sequences. Noncovalent, but specific modification of inorganic particle surfaces represents a generic strategy to modulate functionality and function of nano- or microparticle surfaces.
authors.affiliation: Humboldt-Universität zu Berlin, Department of Chemistry, Laboratory for Organic Synthesis of Functional Systems, Brook-Taylor-Strasse 2, D-12489 Berlin, Germany.
authors.forename: Thorsten
authors.initials: T
authors.lastname: Schwemmer
authors.forename: Jens
authors.initials: J
authors.lastname: Baumgartner
authors.forename: Damien
authors.initials: D
authors.lastname: Faivre
authors.forename: Hans G
authors.initials: HG
authors.lastname: Börner
mesh_terms: D005682:Gadolinium / Q000737:chemistry*; D008958:Models, Molecular; D036103:Nanotechnology; D010316:Particle Size; D010455:Peptides / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D013499:Surface Properties
chemical_list: D010455:Peptides; D011092:Polyethylene Glycols; C030581:gadolinium oxide; D005682:Gadolinium