title: Coronin-1a inhibits autophagosome formation around Mycobacterium tuberculosis-containing phagosomes and assists mycobacterial survival in macrophages.
abstract: Mycobacterium tuberculosis is an intracellular bacterium that can survive within macrophages. Such survival is potentially associated with Coronin-1a (Coro1a). We investigated the mechanism by which Coro1a promotes the survival of M. tuberculosis in macrophages and found that autophagy was involved in the inhibition of mycobacterial survival in Coro1a knock-down (KD) macrophages. Fluorescence microscopy and immunoblot analyses revealed that LC3, a representative autophagic protein, was recruited to M. tuberculosis-containing phagosomes in Coro1a KD macrophages. Thin-section electron microscopy demonstrated that bacilli were surrounded by the multiple membrane structures in Coro1a KD macrophages. The proportion of LC3-positive mycobacterial phagosomes colocalized with p62/SQSTM1, ubiquitin or LAMP1 increased in Coro1a KD macrophages during infection. These results demonstrate the formation of autophagosomes around M. tuberculosis in Coro1a KD macrophages. Phosphorylation of p38 mitogen-activated protein kinase (MAPK) was induced in response to M. tuberculosis infection in Coro1a KD macrophages, suggesting that Coro1a blocks the activation of the p38 MAPK pathway involved in autophagosome formation. LC3 recruitment to M. tuberculosis-containing phagosomes was also observed in Coro1a KD alveolar or bone marrow-derived macrophages. These results suggest that Coro1a inhibits autophagosome formation in alveolar macrophages, thereby facilitating M. tuberculosis survival within the lung.
authors.affiliation: Department of Infectious Diseases, Hamamatsu University School of Medicine, 1-20-1 Handa-yama, Higashi-ku, Hamamatsu 431-3192, Japan. s-seto@hama-med.ac.jp
authors.forename: Shintaro
authors.initials: S
authors.lastname: Seto
authors.forename: Kunio
authors.initials: K
authors.lastname: Tsujimura
authors.forename: Yukio
authors.initials: Y
authors.lastname: Koide
mesh_terms: D000818:Animals; D002460:Cell Line; D055785:Gene Knockdown Techniques; D054884:Host-Pathogen Interactions; D015151:Immunoblotting; D008264:Macrophages / Q000382:microbiology* / Q000648:ultrastructure; D051379:Mice; D050296:Microbial Viability; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism*; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D009169:Mycobacterium tuberculosis / Q000472:pathogenicity* / Q000648:ultrastructure; D010588:Phagosomes / Q000382:microbiology* / Q000648:ultrastructure
chemical_list: D008840:Microfilament Proteins; C072061:coronin proteins