title: Protective effects of β-glucan against oxidative injury induced by 2.45-GHz electromagnetic radiation in the skin tissue of rats.
abstract: In recent times, there is widespread use of 2.45-GHz irradiation-emitting devices in industrial, medical, military and domestic application. The aim of the present study was to investigate the effect of 2.45-GHz electromagnetic radiation (EMR) on the oxidant and antioxidant status of skin and to examine the possible protective effects of β-glucans against the oxidative injury. Thirty-two male Wistar albino rats were randomly divided into four equal groups: control; sham exposed; EMR; and EMR + β-glucan. A 2.45-GHz EMR emitted device from the experimental exposure was applied to the EMR group and EMR + β-glucan group for 60 min daily, respectively, for 4 weeks. β-glucan was administered via gavage at a dose of 50 mg/kg/day before each exposure to radiation in the treatment group. The activities of antioxidant enzymes, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT), as well as the concentration of malondialdehyde (MDA) were measured in tissue homogenates of the skin. Exposure to 2.45-GHz EMR caused a significant increase in MDA levels and CAT activity, while the activities of SOD and GSH-Px decreased in skin tissues. Systemic β-glucan significantly reversed the elevation of MDA levels and the reduction of SOD activities. β-glucan treatment also slightly enhanced the activity of CAT and prevented the depletion of GSH-Px activity caused by EMR, but not statistically significantly. The present study demonstrated the role of oxidative mechanisms in EMR-induced skin tissue damages and that β-glucan could ameliorate oxidative skin injury via its antioxidant properties.
authors.affiliation: Department of Dermatology, Medical Faculty, Suleyman Demirel University, 32200, Cunur, Isparta, Turkey. amuratceyhan@yahoo.com
authors.forename: Ali Murat
authors.initials: AM
authors.lastname: Ceyhan
authors.forename: Vahide Baysal
authors.initials: VB
authors.lastname: Akkaya
authors.forename: Şeyma Celik
authors.initials: ŞC
authors.lastname: Güleçol
authors.forename: Betül Mermi
authors.initials: BM
authors.lastname: Ceyhan
authors.forename: Fehmi
authors.initials: F
authors.lastname: Özgüner
authors.forename: WenChieh
authors.initials: W
authors.lastname: Chen
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D002374:Catalase / Q000378:metabolism; D060733:Electromagnetic Radiation; D004789:Enzyme Activation / Q000187:drug effects; D005979:Glutathione Peroxidase / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D011855:Radiodermatitis / Q000378:metabolism / Q000517:prevention & control*; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000187:drug effects* / Q000293:injuries / Q000378:metabolism / Q000528:radiation effects; D013482:Superoxide Dismutase / Q000378:metabolism; D047071:beta-Glucans / Q000008:administration & dosage*
chemical_list: D000975:Antioxidants; D047071:beta-Glucans; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase