title: [Inhibitory effect of silencing hTERT gene on growth of human squamous cell carcinoma xenograft in nude mice].
abstract: OBJECTIVE
  This study is to explore the inhibitory effect of silencing hTERT gene by short-hairpin RNA on growth of nasopharyngeal carcinoma xenograft in nude mice with RNAi technique.
  
  
  METHODS
  Construction and expression of hTERT cDNA sequence according to the specific hTERT mRNA, including fluorescein eukaryotic expression vector, packaged into a lentivirus. qPCR and Western blot analyzed hTER.T mRNA and protein levels in transfected cells. Proliferation rate of transfected cells was determined by MTT assay in vitro. Cell growth cycle was detected by flow cytometry. The invasiveness of each group was compared using in vitro cell invasion assay.
  
  
  RESULTS
  RT-PCR and Western blot analysis showed that, hTERT siRNA significantly reduced hTERT mRNA and protein levels, especially hTERT siRNA1. siRNA treatment inhibited tumor cell proliferation, and cell migration and invasiveness were significantly lower. Tumor cell growth rate was significantly different between control group and siRNA group (P < 0.01) while tumor cell growth rate in empty virus group(NC group) and control group was not significantly different (P > 0.05).
  
  
  CONCLUSIONS
  Lentivirus containing specific sequences of hTERT gene could significantly inhibit the growth of nasopharyngeal carcinoma cells line. hTERT siRNA expression vector can effectively inhibit NPC cell proliferation, migration and invasion, which may provide a novel molecular targets for gene therapy of nasopharyngeal carcinoma.
authors.affiliation: Department of Otolaryngology, the First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an, 710061, China. yaoxiaobao@sohu.com
authors.forename: Xiaobao
authors.initials: X
authors.lastname: Yao
authors.forename: Xiaoria
authors.initials: X
authors.lastname: Wang
authors.forename: Shaoqiang
authors.initials: S
authors.lastname: Zhang
authors.forename: Liying
authors.initials: L
authors.lastname: Yan
authors.forename: Hongliang
authors.initials: H
authors.lastname: Zhu
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D020868:Gene Silencing; D005822:Genetic Vectors; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D051379:Mice; D008819:Mice, Nude; D009303:Nasopharyngeal Neoplasms / Q000473:pathology*; D009368:Neoplasm Transplantation; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D019098:Telomerase / Q000235:genetics*; D014162:Transfection
chemical_list: D034741:RNA, Small Interfering; D019098:Telomerase