title: [Preparation conditions optimization of Epimedium polysaccharide liposome].
abstract: OBJECTIVE
  To optimize the preparation conditions of Epimedium polysaccharide liposome (EPSL).
  
  
  METHODS
  EPSL was prepared by membrane distribution-supersound method. In preparation of EPSL test, an orthogonal L9 (3(4)) test was used to optimize the preparation conditions, with four factors of ratio of drug to lipid, ratio of lecithin to cholesterol, ultrasonic time and water bath temperature and two indexes of encapsulation efficiency and drug-loading rate. The encapsulation efficiency of EPSL was determined by protamine method.
  
  
  RESULTS
  Optimal preparation condition of EPSL was that ratio of drug to lipid, ratio of lecithin to cholesterol, ultrasonic time and water bath temperature were 1: 30, 4: 1,10 min and 40 degrees C respectively.
  
  
  CONCLUSIONS
  EPSL prepared under Optimal conditions has the property of high encapsulation efficiency and drug-loading rate.
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China. 2009107131@njau.edu.cn
authors.forename: Huan
authors.initials: H
authors.lastname: Gao
authors.forename: Qi
authors.initials: Q
authors.lastname: Gao
authors.forename: De-Yun
authors.initials: DY
authors.lastname: Wang
authors.forename: Yun-Peng
authors.initials: YP
authors.lastname: Fan
authors.forename: Li-Wei
authors.initials: LW
authors.lastname: Guo
authors.forename: Xiao-Na
authors.initials: XN
authors.lastname: Zhao
mesh_terms: D002626:Chemistry, Pharmaceutical; D002784:Cholesterol / Q000008:administration & dosage / Q000737:chemistry; D004337:Drug Carriers; D004339:Drug Compounding / Q000379:methods; D004355:Drug Stability; D034181:Epimedium / Q000737:chemistry*; D054709:Lecithins / Q000008:administration & dosage / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry*; D035261:Plant Components, Aerial / Q000737:chemistry; D011134:Polysaccharides / Q000008:administration & dosage / Q000737:chemistry*; D012995:Solubility; D012997:Solvents / Q000737:chemistry; D013696:Temperature
chemical_list: D004337:Drug Carriers; D054709:Lecithins; D008081:Liposomes; D011134:Polysaccharides; D012997:Solvents; D002784:Cholesterol