title: Experimental characterization of intrapulse tissue conductivity changes for electroporation.
abstract: Cells exposed to short electric pulses experience a change in their transmembrane potential, which can lead to increased membrane permeability of the cell. When the energy of the pulses surpasses a threshold, the cell dies in a non-thermal manner known as irreversible electroporation (IRE). IRE has shown promise in the focal ablation of pathologic tissues. Its non-thermal mechanism spares sensitive structures and facilitates rapid lesion resolution. IRE effects depend on the electric field distribution, which can be predicted with numerical modeling. When the cells become permeabilized, the bulk tissue properties change, affecting this distribution. For IRE to become a reliable and successful treatment of diseased tissues, robust predictive treatment planning methods must be developed. It is vital to understand the changes in tissue properties undergoing the electric pulses to improve numerical models and predict treatment volumes. We report on the experimental characterization of these changes for kidney tissue. Tissue samples were pulsed between plate electrodes while intrapulse voltage and current data were measured to determine the conductivity of the tissue during the pulse. Conductivity was then established as a function of the electric field to which the tissue is exposed. This conductivity curve was used in a numerical model to demonstrate the impact of accounting for these changes when modeling electric field distributions to develop treatment plans.
authors.affiliation: Bioelectromechanical Systems Laboratory, Virginia Tech-Wake Forest School of Biomedical Engineering and Sciences, Virginia Tech, Blacksburg, VA 24060, USA. nealre@vt.edu
authors.forename: Robert E
authors.initials: RE
authors.lastname: Neal
authors.forename: Paulo A
authors.initials: PA
authors.lastname: Garcia
authors.forename: John L
authors.initials: JL
authors.lastname: Robertson
authors.forename: Rafael V
authors.initials: RV
authors.lastname: Davalos
mesh_terms: D000818:Animals; D003198:Computer Simulation; D004307:Dose-Response Relationship, Radiation; D004553:Electric Conductivity; D004574:Electromagnetic Fields; D018274:Electroporation / Q000379:methods*; D066298:In Vitro Techniques; D007679:Kidney Medulla / Q000502:physiology* / Q000528:radiation effects*; D008954:Models, Biological; D011829:Radiation Dosage; D013552:Swine