title: Binding of a truncated form of lecithin:retinol acyltransferase and its N- and C-terminal peptides to lipid monolayers.
abstract: Lecithin:retinol acyltransferase (LRAT) is a 230 amino acid membrane-associated protein which catalyzes the esterification of all-trans-retinol into all-trans-retinyl ester. A truncated form of LRAT (tLRAT), which contains the residues required for catalysis but which is lacking the N- and C-terminal hydrophobic segments, was produced to study its membrane binding properties. Measurements of the maximum insertion pressure of tLRAT, which is higher than the estimated lateral pressure of membranes, and the positive synergy factor a argue in favor of a strong binding of tLRAT to phospholipid monolayers. Moreover, the binding, secondary structure and orientation of the peptides corresponding to its N- and C-terminal hydrophobic segments of LRAT have been studied by circular dichroism and polarization-modulation infrared reflection absorption spectroscopy in monolayers. The results show that these peptides spontaneously bind to lipid monolayers and adopt an α-helical secondary structure. On the basis of these data, a new membrane topology model of LRAT is proposed where its N- and C-terminal segments allow to anchor this protein to the lipid bilayer.
authors.affiliation: LOEX/CUO-recherche, Centre hospitalier affilié universitaire de Québec, Hôpital du Saint-Sacrement, 1050 Chemin Ste-Foy, Québec (Québec), Canada.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Bussières
authors.forename: Line
authors.initials: L
authors.lastname: Cantin
authors.forename: Bernard
authors.initials: B
authors.lastname: Desbat
authors.forename: Christian
authors.initials: C
authors.lastname: Salesse
mesh_terms: D000217:Acyltransferases / Q000737:chemistry / Q000378:metabolism*; D054709:Lecithins; D008055:Lipids / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry*; D008954:Models, Biological; D010455:Peptides; D011485:Protein Binding; D017433:Protein Structure, Secondary; D053835:Unilamellar Liposomes / Q000378:metabolism
chemical_list: D054709:Lecithins; D008055:Lipids; D008565:Membrane Proteins; D010455:Peptides; D053835:Unilamellar Liposomes; D000217:Acyltransferases; C056942:lecithin-retinol acyltransferase