title: ONZIN deficiency attenuates contact hypersensitivity responses in mice.
abstract: ONZIN is abundantly expressed in immune cells of both the myeloid and lymphoid lineage. Expression by lymphoid cells has been reported to further increase after cutaneous exposure of mice to antigens and haptens capable of inducing contact hypersensitivity (CHS), suggesting that ONZIN has a critical role in this response. Here, we report that indeed ONZIN-deficient mice develop attenuated CHS to a number of different haptens. Dampened CHS responses correlated with a significant reduction in pro-inflammatory IL-6 at the challenge site in ONZIN-deficient animals, compared with wild-type controls. Together the study of these animals indicates that loss of ONZIN impacts the effector phase of the CHS response through the regulation of pro-inflammatory factors.
authors.affiliation: Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
authors.forename: Julie G
authors.initials: JG
authors.lastname: Ledford
authors.forename: Martina
authors.initials: M
authors.lastname: Kovarova
authors.forename: Leigh A
authors.initials: LA
authors.lastname: Jania
authors.forename: Mytrang
authors.initials: M
authors.lastname: Nguyen
authors.forename: Beverly H
authors.initials: BH
authors.lastname: Koller
mesh_terms: D019264:Adoptive Transfer / Q000379:methods; D000818:Animals; D017951:Antigen Presentation / Q000276:immunology; D015153:Blotting, Western; D016026:Bone Marrow Transplantation / Q000379:methods; D054360:Chemokine CXCL1 / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D003877:Dermatitis, Contact / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D004431:Ear, External / Q000276:immunology / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015870:Gene Expression / Q000276:immunology; D006241:Haptens / Q000276:immunology*; D018836:Inflammation Mediators / Q000276:immunology / Q000378:metabolism; D015850:Interleukin-6 / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D008198:Lymph Nodes / Q000276:immunology / Q000378:metabolism / Q000637:transplantation; D008297:Male; D051379:Mice; D057507:Mice, 129 Strain; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D015513:Oncogene Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D054360:Chemokine CXCL1; D006241:Haptens; D018836:Inflammation Mediators; D015850:Interleukin-6; D015513:Oncogene Proteins; C506003:onzin protein, mouse