title: Lactoferrin protects against lipopolysaccharide-induced acute lung injury in mice.
abstract: Lactoferrin (LF) plays various anti-inflammatory roles in inflammation experimentally induced by lipopolysaccharides (LPS). But the protective effects of LF on LPS-induced acute lung injury (ALI) have not been elucidated. In this study, we aimed to study the effects of LF on ALI caused by LPS in mice. At 1h before or after LPS injection, an intraperitoneal injection of LF (5mg/body) was administered. Lung specimens and the bronchoalveolar lavage fluid (BALF) were isolated for histopathological examinations and biochemical analyses 12h after LPS exposure. We found that both prophylactic and therapeutic administration of LF significantly decreased the W/D ratio of the lung and protein concentration in the BALF. LF significantly reduced the pulmonary myeloperoxidase activity and the number of total cells in the BALF 12h after LPS challenge. LF treatment markedly attenuated lung edema, alveolar hemorrhage and inflammatory cells infiltration. Moreover, LF also decreased the production of TNF-α and increased interleukin-10 in the BALF. These results firstly indicate that LF may protect against LPS-induced ALI in mice.
authors.affiliation: Department of Pathophysiology, Xuzhou Medical College, Xuzhou, Jiangsu 221002, China.
authors.forename: Xi-jing
authors.initials: XJ
authors.lastname: Li
authors.forename: Da-peng
authors.initials: DP
authors.lastname: Liu
authors.forename: Hua-lin
authors.initials: HL
authors.lastname: Chen
authors.forename: Xiu-hua
authors.initials: XH
authors.lastname: Pan
authors.forename: Qing-yan
authors.initials: QY
authors.lastname: Kong
authors.forename: Qing-feng
authors.initials: QF
authors.lastname: Pang
mesh_terms: D055371:Acute Lung Injury / Q000188:drug therapy / Q000276:immunology / Q000378:metabolism / Q000517:prevention & control*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D006470:Hemorrhage / Q000188:drug therapy / Q000378:metabolism; D016753:Interleukin-10 / Q000276:immunology / Q000378:metabolism; D007781:Lactoferrin / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000276:immunology; D008168:Lung / Q000187:drug effects / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D011654:Pulmonary Edema / Q000188:drug therapy / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D009195:Peroxidase; D007781:Lactoferrin