title: [Screen for natural benzylisoquinoline alkaloids against tumor].
abstract: OBJECTIVE
  To search for natural benzylisoquinoline alkaloids against tumor.
  
  
  METHODS
  In this study, taken DNA as target, a method was developed for screening of natural benzylisoquinoline alkaloids against tumor from traditional Chinese medicine by the use of centrifugal ultrafiltration combined with HPLC technology. The anti-tumor activity for the compounds screened was evaluated in vitro.
  
  
  RESULTS
  Nine compounds interacted with DNA were discovered and identified from Macleaya cordata, Chelidonium majus, Coptis chinensis, and the proliferation of four types of human solid cancer cell lines was markedly inhibited by these compounds.
  
  
  CONCLUSIONS
  The developed method was considered to be suitable for screening of natural benzylisoquinoline alkaloids against tumor from traditional Chinese medicine.
authors.affiliation: Department of Applied Chemistry, College of Chemistry & Molecular Engineering, Peking University, Beijing 100871, China. zzfbj@sina.com
authors.forename: Zhengfu
authors.initials: Z
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Guo
authors.forename: Xionghui
authors.initials: X
authors.lastname: Wei
mesh_terms: D000470:Alkaloids / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D044182:Benzylisoquinolines / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D028281:Chelidonium / Q000737:chemistry*; D039323:Coptis / Q000737:chemistry*; D004353:Drug Evaluation, Preclinical; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D009369:Neoplasms / Q000188:drug therapy / Q000503:physiopathology; D028101:Papaveraceae / Q000737:chemistry*
chemical_list: D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D044182:Benzylisoquinolines; D004365:Drugs, Chinese Herbal