title: Expanding and engineering the genetic code in a single expression experiment.
abstract: Expanding and engineering the code simultaneously: This concept was experimentally realized in a single in vivo expression experiment whereby residue-specific, sense codon reassignments Met→Nle/Pro→(4S-F)Pro (code engineering) were combined with position-specific STOP→Bpa read-through by an amber suppressor tRNA (code expansion).
authors.affiliation: Max Planck Institute of Biochemistry, Molecular Biotechnology, Am Klopferspitz 18, 82152 Martinsried, Germany.
authors.forename: Michael G
authors.initials: MG
authors.lastname: Hoesl
authors.forename: Nediljko
authors.initials: N
authors.lastname: Budisa
mesh_terms: D000596:Amino Acids; D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics; D020869:Gene Expression Profiling; D005815:Genetic Code; D015394:Molecular Structure; D015202:Protein Engineering; D011994:Recombinant Proteins / Q000235:genetics*; D013489:Suppression, Genetic
chemical_list: D000596:Amino Acids; D011994:Recombinant Proteins; D000604:Amino Acyl-tRNA Synthetases