title: Development of a homogeneous immunoassay based on the AlphaLISA method for the detection of chloramphenicol in milk, honey and eggs.
abstract: BACKGROUND
  A homogenous light-induced chemiluminescence immunoassay was developed using AlphaLISA technology for the detection of chloramphenicol (CAP). This technology is based on two different kinds of bead, namely light-sensitive donor beads and beads containing chemiluminescers, also called acceptor beads. A competitive CAP AlphaLISA method was established using artificial antigen-coated acceptor beads, polyclonal antibodies, biotinylated goat anti-rabbit IgG and streptavidin-coated donor beads.
  
  
  RESULTS
  The sensitivity of detection was 0.0086 ng mL⁻¹ and the working range was from 0.0096 to 25 ng mL⁻¹. The intra- and inter-assay coefficients of variation were both below 10%. The average recovery rates at spiked levels of 0.05-10 ng mL⁻¹ were 103.2, 108.4 and 91.6% for milk, honey and eggs respectively. The data obtained from the samples showed good correlation with ELISA results.
  
  
  CONCLUSIONS
  The CAP AlphaLISA method is highly sensitive, specific and rapid and is suitable for screening large quantities of samples.
authors.affiliation: Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi, Jiangsu 214063, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Biao
authors.initials: B
authors.lastname: Huang
authors.forename: Jue
authors.initials: J
authors.lastname: Zhang
authors.forename: Ke
authors.initials: K
authors.lastname: Wang
authors.forename: Jian
authors.initials: J
authors.lastname: Jin
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis; D000941:Antigens / Q000032:analysis; D002701:Chloramphenicol / Q000032:analysis* / Q000276:immunology; D004531:Eggs / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005506:Food Contamination / Q000032:analysis*; D006041:Goats; D006722:Honey / Q000032:analysis*; D007074:Immunoglobulin G / Q000032:analysis; D007158:Immunologic Techniques / Q000379:methods*; D007202:Indicators and Reagents / Q000032:analysis; D049449:Luminescence; D008892:Milk / Q000737:chemistry*; D011817:Rabbits; D019809:Streptavidin / Q000032:analysis
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D002701:Chloramphenicol; D019809:Streptavidin