title: Synthesis and interaction with midkine of biotinylated chondroitin sulfate tetrasaccharides.
abstract: Regiospecifically sulfated chondroitin sulfate repeating tetrasaccharides, CS-OO, GlcAβ-GalNAcβ-GlcAβ-GalNAcβ;CS-EE, GlcAβ-GalNAc(4S6S)β-GlcAβ-GalNAc(4S6S)β; and CS-AA, GlcAβ-GalNAc(4S)β-GlcAβ-GalNAc(4S)β, having biotin linked with a hydrophilic linker at the reducing terminal were synthesized effectively by a coupling of the corresponding disaccharide units and regioselective sulfation. CS-EE showed greater affinity for midkine than CS-AA and CS-OO.
authors.affiliation: Department of Regional Environment, Tottori University, Tottori 680-8551, Japan. jtamura@rstu.jp
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Tamura
authors.forename: Nao
authors.initials: N
authors.lastname: Tsutsumishita-Nakai
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Nakao
authors.forename: Manami
authors.initials: M
authors.lastname: Kawano
authors.forename: Saki
authors.initials: S
authors.lastname: Kato
authors.forename: Naoko
authors.initials: N
authors.lastname: Takeda
authors.forename: Satomi
authors.initials: S
authors.lastname: Nadanaka
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kitagawa
mesh_terms: D019921:Biotinylation; D002240:Carbohydrate Sequence; D002809:Chondroitin Sulfates / Q000138:chemical synthesis* / Q000737:chemistry*; D016207:Cytokines / Q000737:chemistry*; D000078792:Midkine; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000138:chemical synthesis* / Q000737:chemistry*
chemical_list: D016207:Cytokines; D009844:Oligosaccharides; D000078792:Midkine; D002809:Chondroitin Sulfates