title: [Effect of zibuganshenfang on the expression and activity of tyrosinase on B16 cells].
abstract: OBJECTIVE
  ZiBuGanShenFang (ZBGSF) is a traditional herbal formula, which has showed an outstanding therapeutic effect on vitiligo clinically. Our aim is to investigate the influence of ZBGSF drug serum on the expression and activity of Tyrosinase in B16 cells, explore the mechanism of ZBGSF on Vitiligo treatment further.
  
  
  METHODS
  tyrosinase activity was measured by zymological methods, western blotting and RT-PCR were used to measure the protein content and mRNA level of tyrosinase and related proteins, respectively.
  
  
  RESULTS
  ZBGSF drug serum had no effect on the proliferation of B16 cells. But it could promote Tyrosinase activity significantly and increase protein content and mRNA level of tyrosinase and related proteins in B16 cells.
  
  
  CONCLUSIONS
  Promoting the expression of tyrosinase protein and mRNA may be the elementary basis of ZBGSF on Vitiligo treatment.
authors.affiliation: Beijing Hospital of Traditional Chinese Medicine, Capital Medical University Beijing Institute of Traditional Chinese Medicine, Beijing 100010, China.
authors.forename: Jingxia
authors.initials: J
authors.lastname: Zhao
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D008516:Medicine, Chinese Traditional / Q000379:methods; D008546:Melanoma, Experimental / Q000201:enzymology; D051379:Mice; D014442:Monophenol Monooxygenase / Q000187:drug effects*; D014407:Tumor Cells, Cultured; D014820:Vitiligo / Q000188:drug therapy* / Q000201:enzymology*
chemical_list: D004365:Drugs, Chinese Herbal; C569873:zibuganshenfang; D014442:Monophenol Monooxygenase