title: A sensitive chromatographic strip test for the rapid detection of enrofloxacin in chicken muscle.
abstract: A sensitive colloidal gold immunochromatography assay using a specific monoclonal antibody was developed for the rapid detection of enrofloxacin (ENR) residues in chicken muscles. Anti-ENR antibodies with high sensitivity and specificity are generated by immunising BALB/c mice with well-characterised ENR-bovine serum albumin conjugate. An orthogonal L₉(3)³ test was designed, and various parameters that influenced the assay performance were investigated and optimised. Under the optimised conditions, the cut-off limits of semi-quantitative test strips for ENR were found to be 3 ng/mL in phosphate-buffered saline and 8 µg/kg in chicken muscle. The ENR test strips showed a 6% cross-reactivity with ciprofloxacin, 3% with norfloxacin, less than 1% with ofloxacin and sarafloxacin and 0.1% with the other eight fluoroquinolones including enoxacin, difloxacin, danofloxacin, pefloxacin, lomefloxacin, sparfloxacin, oxolinic acid and flumequine. Consistent results are produced from the parallel analysis of ENR-contaminated chicken muscle extracts using test strips and ELISA.
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, P.R. China. yhxiongchen@163.com
authors.forename: Xuelan
authors.initials: X
authors.lastname: Chen
authors.forename: Hengyi
authors.initials: H
authors.lastname: Xu
authors.forename: Weihua
authors.initials: W
authors.lastname: Lai
authors.forename: Yuan
authors.initials: Y
authors.lastname: Chen
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Yang
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Xiong
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis; D000911:Antibodies, Monoclonal / Q000378:metabolism; D000915:Antibody Affinity; D002645:Chickens; D002846:Chromatography, Affinity; D003429:Cross Reactions; D004350:Drug Residues / Q000032:analysis*; D000077422:Enrofloxacin; D005260:Female; D024841:Fluoroquinolones / Q000032:analysis*; D005506:Food Contamination; D005513:Food Inspection / Q000379:methods; D017979:Gold Colloid / Q000737:chemistry; D008460:Meat / Q000032:analysis*; D051379:Mice; D008807:Mice, Inbred BALB C; D018482:Muscle, Skeletal / Q000737:chemistry; D011934:Reagent Strips; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D000911:Antibodies, Monoclonal; D024841:Fluoroquinolones; D017979:Gold Colloid; D011934:Reagent Strips; D000077422:Enrofloxacin