title: ESCRT-III polymers in membrane neck constriction.
abstract: The endosomal sorting complex required for transport (ESCRT)-III machinery contributes to membrane deformation and scission in cytokinesis, intraluminal vesicle formation, autophagy and virus budding. Recombinant ESCRT-III subunits polymerize in vitro into filaments, tubes, sheets or rings, and ESCRT-III-dependent filaments have been observed in cells at virus bud necks and at the cytokinetic abscission site. These observations have inspired speculation about how ESCRT-III could mediate constriction and fission of membrane necks. Based on the polymer structures observed in vitro and in vivo, we discuss models for ESCRT-III function and outline how emerging technologies could be used to test these models.
authors.affiliation: Institute of Biochemistry, Swiss Federal Institute of Technology Zurich (ETHZ), Schafmattstr. 18, CH-8093 Zurich, Switzerland.
authors.forename: Julien
authors.initials: J
authors.lastname: Guizetti
authors.forename: Daniel W
authors.initials: DW
authors.lastname: Gerlich
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D003593:Cytoplasm / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D056827:Endosomal Sorting Complexes Required for Transport / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D055503:Protein Multimerization; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism
chemical_list: D056827:Endosomal Sorting Complexes Required for Transport; D021122:Protein Subunits