title: Comparison of Chloroflexus aurantiacus strain J-10-fl proteomes of cells grown chemoheterotrophically and photoheterotrophically.
abstract: Chloroflexus aurantiacus J-10-fl is a thermophilic green bacterium, a filamentous anoxygenic phototroph, and the model organism of the phylum Chloroflexi. We applied high-throughput, liquid chromatography-mass spectrometry in a global quantitative proteomics investigation of C. aurantiacus cells grown under oxic (chemoorganoheterotrophically) and anoxic (photoorganoheterotrophically) redox states. Our global analysis identified 13,524 high-confidence peptides that matched to 1,286 annotated proteins, 242 of which were either uniquely identified or significantly increased in abundance under photoheterotrophic culture condition. Fifty-four of the 242 proteins are previously characterized photosynthesis-related proteins, including chlorosome proteins, proteins involved in the bacteriochlorophyll biosynthesis, 3-hydroxypropionate (3-OHP) CO(2) fixation pathway, and components of electron transport chains. The remaining 188 proteins have not previously been reported. Of these, five proteins were found to be encoded by genes from a novel operon and observed only in photoheterotrophically grown cells. These proteins candidates may prove useful in further deciphering the phototrophic physiology of C. aurantiacus and other filamentous anoxygenic phototrophs.
authors.affiliation: Biological Separations and Mass Spectrometry, Pacific Northwest National Laboratory, Richland, WA 99352, USA.
authors.forename: Li
authors.initials: L
authors.lastname: Cao
authors.forename: Donald A
authors.initials: DA
authors.lastname: Bryant
authors.forename: Athena A
authors.initials: AA
authors.lastname: Schepmoes
authors.forename: Kajetan
authors.initials: K
authors.lastname: Vogl
authors.forename: Richard D
authors.initials: RD
authors.lastname: Smith
authors.forename: Mary S
authors.initials: MS
authors.lastname: Lipton
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Callister
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D053898:Biosynthetic Pathways / Q000528:radiation effects; D002245:Carbon Dioxide / Q000378:metabolism; D041861:Chloroflexus / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism* / Q000528:radiation effects; D052836:Heterotrophic Processes / Q000528:radiation effects*; D008027:Light; D010455:Peptides / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D015203:Reproducibility of Results
chemical_list: D001426:Bacterial Proteins; D010455:Peptides; D020543:Proteome; D002245:Carbon Dioxide