title: The DYW-class PPR protein MEF7 is required for RNA editing at four sites in mitochondria of Arabidopsis thaliana.
abstract: In plant mitochondria and plastids, RNA editing alters about 400 and about 35 C nucleotides into Us, respectively. Four of these RNA editing events in plant mitochondria specifically require the PPR protein MEF7, characterized by E and DYW extension domains. The gene for MEF7 was identified by genomic mapping of the locus mutated in plants from EMS treated seeds. The SNaPshot screen of the mutant plant population identified two independent EMS mutants with the same editing defects as a corresponding T-DNA insertion line of the MEF7 gene. Although the amino acid codons introduced by the editing events are conserved throughout flowering plants, even the combined failure of four editing events does not impair the growth efficiency of the mutant plants. Five nucleotides are conserved between the four affected editing sites, but are not sufficient for specific recognition by MEF7 since they are also present at three other sites which are unaffected in the mutants.
authors.affiliation: Molekulare Botanik, Universität Ulm, Ulm, Germany.
authors.forename: Anja
authors.initials: A
authors.lastname: Zehrmann
authors.forename: Johannes
authors.initials: J
authors.lastname: van der Merwe
authors.forename: Daniil
authors.initials: D
authors.lastname: Verbitskiy
authors.forename: Barbara
authors.initials: B
authors.lastname: Härtel
authors.forename: Axel
authors.initials: A
authors.lastname: Brennicke
authors.forename: Mizuki
authors.initials: M
authors.lastname: Takenaka
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D017124:Conserved Sequence; D018506:Gene Expression Regulation, Plant; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D017393:RNA Editing; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment
chemical_list: D029681:Arabidopsis Proteins; C575296:MEF7 protein, Arabidopsis; D016601:RNA-Binding Proteins