title: Molecular characterisation of methicillin-sensitive Staphylococcus aureus from deep surgical site infections in orthopaedic patients.
abstract: Staphylococcus aureus is a leading cause of surgical site infections (SSIs). The association between S. aureus genotypes and the severity of illness is, however, incompletely understood. The aim of the study was to genotype S. aureus isolates from deep SSI in orthopaedic patients to identify molecular markers associated with invasive S. aureus infections. DNA microarray analysis was performed on S. aureus isolates collected from 60 patients with deep SSI following major orthopaedic surgery, while 57 isolates from nasal carriers served as controls. Genes associated with antibiotic resistance, adhesion, immune evasion, tissue invasion and toxin production were detected. The bone sialoprotein-binding protein gene (bbp) was more frequent in isolates from SSI patients compared to nasal carriers (95.0% vs. 82.5%), suggesting a role in invasive disease. No major differences in other molecular virulence markers could be distinguished among isolates from the two clinical groups, suggesting that any S. aureus strain may cause invasive infection. Our study reveals important genotypic information on isolates obtained from deep SSI following orthopaedic procedures.
authors.affiliation: Department of Clinical Molecular Biology and Laboratory Sciences (EpiGen), Akershus University Hospital, 1478, Lørenskog, Norway. hege.vangstein.aamot@ahus.no
authors.forename: H V
authors.initials: HV
authors.lastname: Aamot
authors.forename: A
authors.initials: A
authors.lastname: Blomfeldt
authors.forename: I
authors.initials: I
authors.lastname: Skråmm
authors.forename: F
authors.initials: F
authors.lastname: Müller
authors.forename: S
authors.initials: S
authors.lastname: Monecke
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001426:Bacterial Proteins / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics; D002648:Child; D005260:Female; D006801:Humans; D008297:Male; D046228:Microarray Analysis; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D019637:Orthopedic Procedures / Q000009:adverse effects; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D013530:Surgical Wound Infection / Q000382:microbiology*; D037521:Virulence Factors / Q000235:genetics; D055815:Young Adult
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D037521:Virulence Factors; C405490:bone sialoprotein-binding protein, Staphylococcus aureus