title: Proteopolymersomes: in vitro production of a membrane protein in polymersome membranes.
abstract: Polymersomes are stable self-assembled architectures which mimic cell membranes. For characterization, membrane proteins can be incorporated into such bio-mimetic membranes by reconstitution methods, leading to so-called proteopolymersomes. In this work, we demonstrate the direct incorporation of a membrane protein into polymersome membranes by a cell-free expression system. Firstly, we demonstrate pore formation in the preformed polymersome membrane using α-hemolysin. Secondly, we use claudin-2, a protein involved in cell-cell interactions, to demonstrate the in vitro expression of a membrane protein into these polymersomes. Surface plasmon resonance (Biacore) binding studies with the claudin-2 proteopolymersomes and claudin-2 specific antibodies are performed to show the presence of the in vitro expressed protein in polymersome membranes.
authors.affiliation: Institute of Materials Research and Engineering, A∗STAR, Singapore, Singapore.
authors.forename: Madhavan
authors.initials: M
authors.lastname: Nallani
authors.forename: Mirjam
authors.initials: M
authors.lastname: Andreasson-Ochsner
authors.forename: Cherng-Wen Darren
authors.initials: CW
authors.lastname: Tan
authors.forename: Eva-Kathrin
authors.initials: EK
authors.lastname: Sinner
authors.forename: Yudi
authors.initials: Y
authors.lastname: Wisantoso
authors.forename: Susana
authors.initials: S
authors.lastname: Geifman-Shochat
authors.forename: Walter
authors.initials: W
authors.lastname: Hunziker
mesh_terms: D057167:Claudins / Q000378:metabolism*; D005452:Fluoresceins / Q000378:metabolism; D005453:Fluorescence; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D011108:Polymers / Q000378:metabolism*; D020349:Surface Plasmon Resonance
chemical_list: C491875:CLDN2 protein, human; D057167:Claudins; D005452:Fluoresceins; D008565:Membrane Proteins; D008567:Membranes, Artificial; D011108:Polymers; C007740:fluorexon