title: Chemometric modeling and two-dimensional fluorescence analysis of bioprocess with a new strain of Klebsiella pneumoniae to convert residual glycerol into 1,3-propanediol.
abstract: The goal of this study was to show that the metabolism of Klebsiella pneumoniae under different aeration strategies could be monitored and predicted by the application of chemometric models and fluorescence spectroscopy. Multi-wavelength fluorescence was applied to the on-line monitoring of process parameters for K.                         pneumoniae cultivations. Differences observed in spectra collected under aerobiosis and anaerobiosis can be explained by the different metabolic states of the cells. To predict process variables such as biomass, glycerol, and 1,3-propanediol (1,3-PD), chemometric models were developed on the basis of the acquired fluorescence spectra, which were measured continuously. Although glycerol and 1,3-PD are not fluorescent compounds, the results showed that this technique could be successfully applied to the on-line monitoring of variables in order to understand the process and thus improve 1,3-PD production. The root mean square errors of predictions were 0.78 units, 10 g/L, and 2.6 g/L for optical density, glycerol, and 1,3-PD, respectively.
authors.affiliation: Biotechnology & Biochemical Engineering Laboratory-BiotecLab, Federal University of Rio Grande do Sul, Av. Bento Gonçalves, 9500, P.O. Box 15090, Porto Alegre, RS 91501-970, Brazil. dmisturini@gmail.com
authors.forename: Daniele
authors.initials: D
authors.lastname: Misturini Rossi
authors.forename: Dörte
authors.initials: D
authors.lastname: Solle
authors.forename: Bernd
authors.initials: B
authors.lastname: Hitzmann
authors.forename: Marco Antônio Záchia
authors.initials: MA
authors.lastname: Ayub
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D018533:Biomass; D005453:Fluorescence; D005990:Glycerol / Q000378:metabolism*; D007711:Klebsiella pneumoniae / Q000378:metabolism*; D016018:Least-Squares Analysis; D015233:Models, Statistical; D011409:Propylene Glycols / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D011409:Propylene Glycols; C041787:1,3-propanediol; D005990:Glycerol