title: Combination of biological pretreatment with liquid hot water pretreatment to enhance enzymatic hydrolysis of Populus tomentosa.
abstract: A novel stepwise pretreatment of combination of fungal treatment with liquid hot water (LHW) treatment was conducted to enhance the enzymatic hydrolysis of Populus tomentosa. The results showed that lignin and cellulose increased with the elevating temperature, while significant amount of hemicellulose was degraded during the LHW pretreatment. A highest hemicellulose removal of 92.33% was observed by combination of Lenzites betulina C5617 with LHW treatment at 200°C, which was almost 2 times higher than that of sole LHW treatment at the same level. Saccharification of poplar co-treated with L. betulina C5617 and LHW at 200°C resulted in a 2.66-fold increase of glucose yield than that of sole LHW treatment, and an increase (2.25-fold) of glucose yield was obtained by the combination of Trametes ochracea C6888 with LHW. The combination pretreatment performed well at accelerating the enzymatic hydrolysis of poplar wood.
authors.affiliation: Institute of Microbiology, Beijing Forestry University, Beijing 100083, China. wish101@yahoo.cn
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Tongqi
authors.initials: T
authors.lastname: Yuan
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
authors.forename: Baokai
authors.initials: B
authors.lastname: Cui
authors.forename: Yucheng
authors.initials: Y
authors.lastname: Dai
mesh_terms: D004798:Enzymes / Q000737:chemistry*; D006358:Hot Temperature; D006868:Hydrolysis; D032107:Populus / Q000737:chemistry*; D014867:Water
chemical_list: D004798:Enzymes; D014867:Water