title: HIV-1 neutralization coverage is improved by combining monoclonal antibodies that target independent epitopes.
abstract: HIV-1 neutralizing monoclonal antibodies (MAbs) define key targets for vaccine development and are being considered for passive prevention of infection. We analyzed the interaction of MAbs to two independent epitopes on the viral envelope glycoprotein. Potently neutralizing MAbs to the CD4 binding site and V1V2 region displayed no in vitro cross-competition and displayed additive, though not synergistic, neutralization activity. Predicted neutralization coverage of a combination of two MAbs reached 97% on a 208-isolate panel.
authors.affiliation: Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: Nicole A
authors.initials: NA
authors.lastname: Doria-Rose
authors.forename: Mark K
authors.initials: MK
authors.lastname: Louder
authors.forename: Zhongjia
authors.initials: Z
authors.lastname: Yang
authors.forename: Sijy
authors.initials: S
authors.lastname: O'Dell
authors.forename: Martha
authors.initials: M
authors.lastname: Nason
authors.forename: Stephen D
authors.initials: SD
authors.lastname: Schmidt
authors.forename: Krisha
authors.initials: K
authors.lastname: McKee
authors.forename: Michael S
authors.initials: MS
authors.lastname: Seaman
authors.forename: Robert T
authors.initials: RT
authors.lastname: Bailer
authors.forename: John R
authors.initials: JR
authors.lastname: Mascola
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D057134:Antibodies, Neutralizing / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D015483:HIV Antibodies / Q000276:immunology*; D015699:HIV Envelope Protein gp120 / Q000276:immunology; D015658:HIV Infections / Q000276:immunology / Q000821:virology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans
chemical_list: D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing; D000939:Epitopes; D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120