title: RACK1 downregulation suppresses migration and proliferation of neuroblastoma             cell lines.
abstract: Neuroblastoma is the most common extracranial solid tumor in childhood,             whose molecular mechanism on clinically heterogeneous behavior is still unclear.             Receptor for activated C-kinase 1 (RACK1) has been shown to be involved in the             regulation of growth and migration in many types of cells. Two types of human             neuroblastoma cell lines, SK-N-SH and SK-N-BE (2), were used to explore the role             of RACK1 in neuroblastoma cell migration and proliferation. Cell migration and             proliferation were detected by a transwell system and colorimetric bromodeoxyuridine             (BrdU) ELISA, respectively. The expressions of RACK1, N-Myc, phospho-Src(Tyr416),             phospho-Src(Tyr527), phospho-Akt, phospho-ERK1/2 and phospho-p38 were analyzed             by Western blotting. The expression of RACK1 was then repressed by target RNA             interference (RNAi) in both types of neuroblastoma cells, and the expression of             signaling molecules, migration and proliferation of cells was analyzed. Compared             with SK-N-SH, the migration and proliferation of SK-N-BE(2) cells was remarkably             higher which was accompanied by higher expression of RACK1 and phospho-Src(Tyr416).             RACK1 RNAi repressed cell migration and proliferation, and decreased the expression             of phospho-Src(Tyr416) in both cell lines. In summary, RACK1 was expressed in             the neuroblastoma cells and positively regulated cell migration and proliferation             probably via modulating the activation of Src on Tyr416 residue.
authors.affiliation: Department of Pediatric Surgery, Shanghai Xinhua Hospital, Affiliated with Shanghai Jiao Tong University, Shanghai 200092, PR China.
authors.forename: Fan
authors.initials: F
authors.lastname: Lu
authors.forename: Chi
authors.initials: C
authors.lastname: Zhang
authors.forename: Wen-Jie
authors.initials: WJ
authors.lastname: Wu
authors.forename: Ye-Ming
authors.initials: YM
authors.lastname: Wu
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D048049:Extracellular Signal-Regulated MAP Kinases / Q000235:genetics; D019204:GTP-Binding Proteins / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D016259:Genes, myc; D016391:Genes, src; D006801:Humans; D009363:Neoplasm Proteins / Q000235:genetics*; D009447:Neuroblastoma / Q000235:genetics*; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics; D000075370:Receptors for Activated C Kinase; D011956:Receptors, Cell Surface / Q000235:genetics*; D048051:p38 Mitogen-Activated Protein Kinases / Q000235:genetics
chemical_list: D009363:Neoplasm Proteins; C419368:RACK1 protein, human; D000075370:Receptors for Activated C Kinase; D011956:Receptors, Cell Surface; D051057:Proto-Oncogene Proteins c-akt; D048049:Extracellular Signal-Regulated MAP Kinases; D048051:p38 Mitogen-Activated Protein Kinases; D019204:GTP-Binding Proteins