title: [Expression of RUNX3 in cervical carcinoma  and its clinical significance].
abstract: OBJECTIVE To explore the role of runt-related transcription factor 3(RUNX3) in the tumorgenesis and progression of cervical carcinoma. METHODS The immunohistochemical staining technique was used to detect the expression of RUNX3 protein in 25 cases of normal cervix, 34 intraepithelia neoplasia (CIN), and 48 cervical carcinomas. SYBR Green I chimeric fluorescence Real-time PCR was applied to detect the expression of RUNX3 mRNA in 10 cases of normal cervix, 24 CIN, and 30 cervical carcinomas. RESULTS The expressions of RUNX3 protein and mRNA in normal cervix, CINI,CINII-III, and cervical carcinoma tissues tended to be down-regulated. There was significant difference among these groups (P<0.05). The expressions of RUNX3 protein and mRNA in the cervical carcinoma tissues were correlated with the histological differentiation, clinical stage, and lymphatic metastasis (P<0.05), but had no relationship with the age, high-risk human papillomavirus infection, and histological classification (P> 0.05). CONCLUSION RUNX3 may function as a tumor suppressor gene in the occurrence and progression of cervical carcinoma.
authors.affiliation: Department of Obstetrics and Gynecology, Second Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Yuping
authors.initials: Y
authors.lastname: Deng
authors.forename: Meifang
authors.initials: M
authors.lastname: Nie
authors.forename: Fengying
authors.initials: F
authors.lastname: Liu
authors.forename: Shan
authors.initials: S
authors.lastname: Jiang
authors.forename: Yizhi
authors.initials: Y
authors.lastname: Liu
authors.forename: Dengqing
authors.initials: D
authors.lastname: Li
authors.forename: Qucheng
authors.initials: Q
authors.lastname: Meng
authors.forename: Jin
authors.initials: J
authors.lastname: Li
authors.forename: Minzhu
authors.initials: M
authors.lastname: Huang
authors.forename: Mingming
authors.initials: M
authors.lastname: Wang
mesh_terms: D000328:Adult; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism*; D050677:Core Binding Factor Alpha 3 Subunit / Q000235:genetics / Q000378:metabolism*; D018450:Disease Progression; D005260:Female; D006801:Humans; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000378:metabolism*; D002578:Uterine Cervical Dysplasia / Q000235:genetics / Q000378:metabolism
chemical_list: D050677:Core Binding Factor Alpha 3 Subunit; D012333:RNA, Messenger; C493729:Runx3 protein, human