title: [Rapid identification for Paenibacillus mucilaginosus by PCR].
abstract: OBJECTIVE
  Paenibacillus mucilaginosus is widely used as microbial fertilizers, therefore, rapid identification of this species by Polymerase Chain Reaction (PCR) is necessary for detection and ecological evaluation of microbial fertilizer.
  
  
  METHODS
  A species-specific primer pair of P. mucilaginosus, orf06701-F(5'-ATGGAGGAAACATGGGGTGA-3')/orf06701-R (5'-TCAGGAATGAAGGCCCCCTT-3') was designed. Optimization of PCR, specificity and sensitivity determination were followed and the rapid identification was established.
  
  
  RESULTS
  A single band with 333 bp was consistently amplified from all the strains of P. mucilaginosus tested and negative results were obtained from all the reference strains, such as other strains of Paenibacillus and Bacillus. The PCR detection limitation was 400 - 1000 cells per assay, indicating the sensitivity of the rapid culture-PCR method. To verify the rapid identification, soil was sampled and cultured. Five of 11 soil isolates were rapidly identified as P. mucilaginosus by the method, and the similarity of 333bp sequences were 100%.
  
  
  CONCLUSIONS
  The methods can be used in the rapid identification of P. mucilaginosus and the results will provide technical supports for the detection and ecological evaluation of microbial fertilizer.
authors.affiliation: College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Wang
authors.forename: Mingchao
authors.initials: M
authors.lastname: Ma
authors.forename: Dawei
authors.initials: D
authors.lastname: Guan
authors.forename: Xin
authors.initials: X
authors.lastname: Jiang
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Yanqin
authors.initials: Y
authors.lastname: Ding
authors.forename: Jun
authors.initials: J
authors.lastname: Li
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D005308:Fertilizers; D056507:Paenibacillus / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D005308:Fertilizers