title: Isolation and characterization of a murine P388 leukemia line resistant to thiarabine.
abstract: A murine P388 leukemia line fully resistant to thiarabine was obtained after five courses of intraperitoneal treatment (daily for nine consecutive days). The subline was sensitive as was the parental P388/0 line to 5-fluorouracil, gemcitabine, cyclophosphamide, cisplatin, melphalan, BCNU, mitomycin C, doxorubicin, mitoxantrone, etoposide, irinotecan, vincristine, and paclitaxel, but was cross resistant (at least marginally) to three antimetabolites: palmO-ara-C, fludarabine phosphate, and methotrexate. The deoxycytidine kinase activity in the subline was comparable to that for P388/0, whereas the dCMP deaminase activity was 43% of that for P388/0. No deoxycytidine deaminase activity was detected in either of the leukemias. There appeared to be little, if any, difference in the metabolism of deoxycytidine, cytidine, or thiarabine in the two leukemias.
authors.affiliation: Cancer Therapeutics and Immunology Department, Southern Research Institute, Birmingham, Alabama 35255-5305, USA. waud@southernresearch.org
authors.forename: William R
authors.initials: WR
authors.lastname: Waud
authors.forename: William B
authors.initials: WB
authors.lastname: Parker
authors.forename: Karen S
authors.initials: KS
authors.lastname: Gilbert
authors.forename: John A
authors.initials: JA
authors.lastname: Secrist
mesh_terms: D000818:Animals; D000963:Antimetabolites / Q000008:administration & dosage* / Q000138:chemical synthesis; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000737:chemistry; D001088:Arabinonucleotides / Q000008:administration & dosage* / Q000138:chemical synthesis; D045744:Cell Line, Tumor / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D003844:DCMP Deaminase / Q000378:metabolism; D003842:Deoxycytidine Kinase / Q000378:metabolism; D019008:Drug Resistance, Neoplasm / Q000187:drug effects*; D005260:Female; D007941:Leukemia P388; D051379:Mice; D009368:Neoplasm Transplantation; D014183:Transplantation, Heterologous
chemical_list: D000963:Antimetabolites; D000970:Antineoplastic Agents; D001088:Arabinonucleotides; D003842:Deoxycytidine Kinase; D003844:DCMP Deaminase