title: Application of subproteomics in the characterization of Gram-positive bacteria.
abstract: Gram-positive bacteria cause a series of diseases in human, animals and plants. There has been increasing interest in efforts to investigate pathogenesis of bacteria using multiple "omic" strategies including proteomics. Proteins in different cell fractions of bacteria may play different vital roles in various physiological processes, such as adhesion, invasion, internalization, sensing, respiration, oxidative stress protection and pathogenicity. Subproteomics specifically focuses on the pre-fractionated cellular proteins and thus may be able to characterize more low-abundance molecules that are usually overlooked by the traditional whole-cell proteomics, providing comprehensive information for further investigations. This review intends to outline the current progress, challenges and future development of subproteomics in the characterization of Gram-positive bacteria. This article is part of a Special Issue entitled: Proteomics: The clinical link.
authors.affiliation: Institute of Life and Health Engineering/National Engineering & Research Center of Genetic Medicine, College of Life Sciences and Technology, Jinan University, Guangzhou 510632, China.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Yang
authors.forename: Jie
authors.initials: J
authors.lastname: Lu
authors.forename: Xuesong
authors.initials: X
authors.lastname: Sun
authors.forename: Qing-Yu
authors.initials: QY
authors.lastname: He
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods*; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D002473:Cell Wall / Q000737:chemistry / Q000378:metabolism; D006094:Gram-Positive Bacteria / Q000737:chemistry / Q000145:classification* / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D008954:Models, Biological; D040901:Proteomics / Q000379:methods*