title: Microbial transformation of deoxyandrographolide and their inhibitory activity on LPS-induced NO production in RAW 264.7 macrophages.
abstract: A series of analogues of deoxyandrographolide (1) transformed by Cunninghamella blakesleana AS 3.2004 were isolated and identified by spectral methods including 2D NMR. Among them, 3-oxo-17,19-dihydroxy-7,13-ent-labdadien-15,16-olide (9), 3-oxo-19-hydroxy-1,13-ent-labdadien-15,16-olide (16), 3-oxo-1β-hydroxy-14-deoxy-andrographolide (17) and 3-oxo-2β-hydroxy-14-deoxyandrographolide (18) are new compounds. And their structure-activity relationships (SAR) of inhibitory activity on LPS-induced NO production in RAW 264.7 macrophage cells were also discussed.
authors.affiliation: School of Pharmaceutical Sciences, Dalian Medical University, Dalian 116044, PR China.
authors.forename: Sa
authors.initials: S
authors.lastname: Deng
authors.forename: Bao Jing
authors.initials: BJ
authors.lastname: Zhang
authors.forename: Chang Yuan
authors.initials: CY
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Tian
authors.forename: Ji Hong
authors.initials: JH
authors.lastname: Yao
authors.forename: Lei
authors.initials: L
authors.lastname: An
authors.forename: Shan Shan
authors.initials: SS
authors.lastname: Huang
authors.forename: Jin Yong
authors.initials: JY
authors.lastname: Peng
authors.forename: Ke Xin
authors.initials: KX
authors.lastname: Liu
authors.forename: Xiao Chi
authors.initials: XC
authors.lastname: Ma
mesh_terms: D020093:Cunninghamella / Q000201:enzymology / Q000378:metabolism; D004224:Diterpenes / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D008070:Lipopolysaccharides; D008264:Macrophages / Q000187:drug effects*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009569:Nitric Oxide / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D013329:Structure-Activity Relationship
chemical_list: C114791:14-deoxyandrographolide; D004224:Diterpenes; D008070:Lipopolysaccharides; D009569:Nitric Oxide