title: DNA-damage effect of polycyclic aromatic hydrocarbons from urban area, evaluated in lung fibroblast cultures.
abstract: This study was designed to biomonitor the effect of PAH extracts from urban areas on the DNA of lung cell cultures. The analyses of the polycyclic aromatic hydrocarbons (PAHs) were performed in atmospheric PM(2.5) and PM(10) collected at three sampling sites with heavy traffic located in the Metropolitan Area of Porto Alegre (MAPA) (Brazil). The concentrations of 16 major PAHs were determined according to EPA. Comet assay on V79 hamster lung cells was chosen for genotoxicity evaluation. Temperature, humidity, and wind speed were recorded. With regard to the damage index, higher levels were reported in the extract of particulate matter samples from the MAPA during the summer. High molecular weight compounds showed correlation with DNA damage frequency and their respective carcinogenicity.
authors.affiliation: Fundação Estadual de Proteção Ambiental Henrique Luis Roessler, FEPAM, Porto Alegre, RS, Brazil. gerpro.pesquisa@fepam.rs.gov.br
authors.forename: Elba Calesso
authors.initials: EC
authors.lastname: Teixeira
authors.forename: Daniel
authors.initials: D
authors.lastname: Pra
authors.forename: Daniele
authors.initials: D
authors.lastname: Idalgo
authors.forename: João Antonio Pêgas
authors.initials: JA
authors.lastname: Henriques
authors.forename: Flavio
authors.initials: F
authors.lastname: Wiegand
mesh_terms: D000393:Air Pollutants / Q000032:analysis / Q000633:toxicity*; D000818:Animals; D001938:Brazil; D006224:Cricetinae; D004249:DNA Damage / Q000187:drug effects*; D004784:Environmental Monitoring / Q000379:methods*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D008168:Lung / Q000166:cytology / Q000187:drug effects*; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000633:toxicity*; D012621:Seasons; D014504:Urban Health
chemical_list: D000393:Air Pollutants; D011084:Polycyclic Aromatic Hydrocarbons