title: Analysis of cholesterol oxidation products by Fast gas chromatography/mass spectrometry.
abstract: The aim of the present study was to set-up a Fast gas chromatography/mass spectrometry method for the analysis of cholesterol oxidation products (COPs). A silylated mixture of seven oxysterol standards was injected into a Fast GC/MS system. A capillary GC column (10 m×0.1 mm internal diameter×0.1 μm film thickness) coated with 95% dimethyl- and 5% diphenyl-polysiloxane, was used. The method gave a fast (total analysis time=3.5 min) and satisfactory resolution (R>1.2) of the COPs standards, with a good repeatability and sensitivity, similar to those of conventional GC/MS; recoveries were tested on mice liver. Fast GC/MS method suitability for COPs analysis in food was also tested on an oxidized sardine fillet, which had been previously saponified and purified by NH(2)  solid-phase extraction (SPE); a good repeatability and sensitivity was also obtained. The analytical performance of the Fast GC/MS method for the determination of COPs, together with the consequent significant reduction of the analysis time and consumables, demonstrates that Fast GC/MS represents a valid alternative to conventional GC/MS and evinces the great potential of such an analytical technique, which could be applied for both food and biological samples.
authors.affiliation: Department of Food Science, Alma Mater Studiorum-University of Bologna, Bologna, Italy.
authors.forename: Vladimiro
authors.initials: V
authors.lastname: Cardenia
authors.forename: Maria T
authors.initials: MT
authors.lastname: Rodriguez-Estrada
authors.forename: Elena
authors.initials: E
authors.lastname: Baldacci
authors.forename: Stefano
authors.initials: S
authors.lastname: Savioli
authors.forename: Giovanni
authors.initials: G
authors.lastname: Lercker
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000031:analogs & derivatives / Q000032:analysis* / Q000737:chemistry*; D005399:Fishes; D008401:Gas Chromatography-Mass Spectrometry; D008099:Liver / Q000737:chemistry; D008461:Meat Products / Q000032:analysis; D051379:Mice; D010084:Oxidation-Reduction
chemical_list: D002784:Cholesterol