title: Detection of gastric cancer-associated microRNAs on microRNA microarray comparing pre- and post-operative plasma.
abstract: BACKGROUND
  Recently, it was reported that plasma microRNAs (miRNAs) are low-invasive useful biomarkers for cancer. We attempted to isolate gastric cancer (GC)-associated miRNAs comparing pre- and post-operative paired plasma, thereby excluding the possible effects of individual variability.
  
  
  METHODS
  This study was divided into four steps: (1) microarray analysis comparing pre- and post-operative plasma; (2) validation of candidate miRNAs by quantitative RT-PCR; (3) validation study of selected miRNAs using paired plasma; and (4) comparison of the levels of selected miRNAs in plasma between healthy controls and patients.
  
  
  RESULTS
  From the results of microarray analysis, nine candidate miRNAs the levels of which were markedly decreased in post-operative plasma were selected for further studies. After confirmation of their post-operative marked reduction, two candidate miRNAs, miR-451 and miR-486, were selected as plasma biomarkers, considering the abundance in plasma, and marked decrease in post-operative samples. In validation, the two miRNAs were found to decrease in post-operative plasma in 90 and 93% of patients (both P<0.01). In comparison with healthy controls, the levels of both miRNAs were found to be significantly higher in patients, and the area under the curve values were high at 0.96 and 0.92.
  
  
  CONCLUSIONS
  Plasma miR-451 and miR-486 could be useful blood-based biomarkers for screening GC.
authors.affiliation: Division of Digestive Surgery, Department of Surgery, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Kawaramachihirokoji, Kamigyo-ku, Kyoto 602-8566, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Konishi
authors.forename: D
authors.initials: D
authors.lastname: Ichikawa
authors.forename: S
authors.initials: S
authors.lastname: Komatsu
authors.forename: A
authors.initials: A
authors.lastname: Shiozaki
authors.forename: M
authors.initials: M
authors.lastname: Tsujiura
authors.forename: H
authors.initials: H
authors.lastname: Takeshita
authors.forename: R
authors.initials: R
authors.lastname: Morimura
authors.forename: H
authors.initials: H
authors.lastname: Nagata
authors.forename: T
authors.initials: T
authors.lastname: Arita
authors.forename: T
authors.initials: T
authors.lastname: Kawaguchi
authors.forename: S
authors.initials: S
authors.lastname: Hirashima
authors.forename: H
authors.initials: H
authors.lastname: Fujiwara
authors.forename: K
authors.initials: K
authors.lastname: Okamoto
authors.forename: E
authors.initials: E
authors.lastname: Otsuji
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood; D055088:Early Detection of Cancer; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D046228:Microarray Analysis; D011184:Postoperative Period; D057234:Preoperative Period; D013274:Stomach Neoplasms / Q000097:blood / Q000235:genetics* / Q000601:surgery; D054928:Validation Studies as Topic
chemical_list: D014408:Biomarkers, Tumor; C531201:MIRN451 microRNA, human; C560945:MIRN486 microRNA, human; D035683:MicroRNAs