title: Beet necrotic yellow vein virus subgenomic RNA3 is a cleavage product leading to stable non-coding RNA required for long-distance movement.
abstract: Beet necrotic yellow vein virus (BNYVV) is a multipartite RNA virus. BNYVV RNA3 does not accumulate in non-host transgenic Arabidopsis thaliana plants when expressed using a 35S promoter. However, a 3'-derivative species has been detected in transgenic plants and in transient expression assays conducted in Nicotiana benthamiana and Beta macrocarpa. The 3'-derivative species is similar to the previously reported subgenomic RNA3 produced during virus infection. 5' RACE revealed that the truncated forms had identical 5' ends. The 5' termini carried the coremin motif also present on BNYVV RNA5, beet soil-borne mosaic virus RNA3 and 4, and cucumber mosaic virus group 2 RNAs. This RNA3 species lacks a m(7)Gppp at the 5' end of the cleavage products, whether expressed transiently or virally. Mutagenesis revealed the importance of the coremin sequence for both long-distance movement and stabilization of the cleavage product in vivo and in vitro. The isolation of various RNA3 5'-end products suggests the existence of a cleavage between nt 212 and 1234 and subsequent exonucleolytic degradation, leading to the accumulation of a non-coding RNA. When RNA3 was incubated in wheatgerm extracts, truncated forms appeared rapidly and their appearance was protein- and divalent ion-dependent.
authors.affiliation: Institut de Biologie Moléculaire des Plantes, Laboratoire Propre du CNRS (UPR 2357) conventionné avec l'Université de Strasbourg, 12 Rue du Général Zimmer, 67084 Strasbourg, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Peltier
authors.affiliation: Institut de Biologie Moléculaire des Plantes, Laboratoire Propre du CNRS (UPR 2357) conventionné avec l'Université de Strasbourg, 12 Rue du Général Zimmer, 67084 Strasbourg, France.
authors.forename: Elodie
authors.initials: E
authors.lastname: Klein
authors.affiliation: Institut de Biologie Moléculaire des Plantes, Laboratoire Propre du CNRS (UPR 2357) conventionné avec l'Université de Strasbourg, 12 Rue du Général Zimmer, 67084 Strasbourg, France.
authors.forename: Kamal
authors.initials: K
authors.lastname: Hleibieh
authors.affiliation: Università di Bologna, Dipartimento di Scienze e Tecnologie Agroambientali, Area Patologia Vegetale, Viale G. Fanin 40, II piano, 40127 Bologna, Italy.
authors.forename: Massimiliano
authors.initials: M
authors.lastname: D'Alonzo
authors.affiliation: Institut de Biologie Moléculaire et Cellulaire, Laboratoire Propre du CNRS conventionné avec l'Université de Strasbourg, 15 Rue René Descartes, 67084 Strasbourg, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Hammann
authors.affiliation: Institut de Biologie Moléculaire des Plantes, Laboratoire Propre du CNRS (UPR 2357) conventionné avec l'Université de Strasbourg, 12 Rue du Général Zimmer, 67084 Strasbourg, France.
authors.forename: Salah
authors.initials: S
authors.lastname: Bouzoubaa
authors.affiliation: Università di Bologna, Dipartimento di Scienze e Tecnologie Agroambientali, Area Patologia Vegetale, Viale G. Fanin 40, II piano, 40127 Bologna, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Ratti
authors.affiliation: Institut de Biologie Moléculaire des Plantes, Laboratoire Propre du CNRS (UPR 2357) conventionné avec l'Université de Strasbourg, 12 Rue du Général Zimmer, 67084 Strasbourg, France.
authors.forename: David
authors.initials: D
authors.lastname: Gilmer
mesh_terms: D017360:Arabidopsis / Q000821:virology; D027461:Beta vulgaris / Q000821:virology; D016679:Genome, Viral; D010942:Plant Viruses / Q000235:genetics / Q000472:pathogenicity*; D020871:RNA Stability; D022661:RNA, Untranslated / Q000378:metabolism*; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism*; D014026:Nicotiana / Q000821:virology
chemical_list: D022661:RNA, Untranslated; D012367:RNA, Viral