title: Comparison of four current 25-hydroxyvitamin D assays.
abstract: OBJECTIVE
  The performance of recently developed vitamin D total assays (ADVIA Centaur and Elecsys) was compared to that of liquid chromatography-tandem mass spectrometry (LC-MS/MS) and LIASON 25-OH Vitamin D total assays.
  
  
  METHODS
  A total of 157 clinical samples and standard reference material (SRM) 972 were analyzed.
  
  
  RESULTS
  The correlations of LC-MS/MS with the three immunoassays were acceptable. However, compared to LC-MS/MS, LIAISON and ADVIA Centaur showed negative bias, and Elecsys showed positive bias. There was a lack of agreement among the four methods with only LC-MS/MS results close to the certified values of SRM 972. The prevalence of vitamin D insufficiency (<50 nmol/L) was higher with ADVIA Centaur (51.6%) and LIAISON (52.2%) and lower with Elecsys (37.6%), compared with that of LC-MS/MS (44.6%).
  
  
  CONCLUSIONS
  The new, automated total vitamin D assays show acceptable correlation with LC-MS/MS, and could be used in routine laboratories. However, standardization of vitamin D assays and consideration of assay-specific decision limits should be addressed.
authors.affiliation: Department of Laboratory Medicine, Konkuk University School of Medicine, 4–12 Hwayang-dong, Gwangjin-gu, Seoul, South Korea.
authors.forename: Hee-Won
authors.initials: HW
authors.lastname: Moon
authors.forename: Jin-Hee
authors.initials: JH
authors.lastname: Cho
authors.forename: Mina
authors.initials: M
authors.lastname: Hur
authors.forename: Junghan
authors.initials: J
authors.lastname: Song
authors.forename: Gwi Young
authors.initials: GY
authors.lastname: Oh
authors.forename: Chul Min
authors.initials: CM
authors.lastname: Park
authors.forename: Yeo-Min
authors.initials: YM
authors.lastname: Yun
authors.forename: Jin Q
authors.initials: JQ
authors.lastname: Kim
mesh_terms: D015652:25-Hydroxyvitamin D 2 / Q000097:blood*; D057205:Automation, Laboratory; D002112:Calcifediol / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D003429:Cross Reactions; D006801:Humans; D007118:Immunoassay; D008163:Luminescent Measurements; D015995:Prevalence; D015203:Reproducibility of Results; D056910:Republic of Korea / Q000453:epidemiology; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D014808:Vitamin D Deficiency / Q000097:blood* / Q000175:diagnosis* / Q000453:epidemiology
chemical_list: D015652:25-Hydroxyvitamin D 2; D002112:Calcifediol