title: Raman based hepatocellular carcinoma biomarker detection.
abstract: Highly sensitive and selective biomarker detection is required for early detection of hepatocellular carcinoma (HCC). Disease progression has been shown to correlate with specific fucosylation of a validated HCC serum glycoprotein biomarker, alpha-fetoprotein (AFP) Carbohydrate binding proteins, such as lectins, can be used as diagnostic indicators for monitoring glycosylation changes during disease progression in hepatitis B virus (HBV) or hepatitis C virus (HCV) infected patients. We prepared surface-enhanced Raman spectroscopy (SERS) substrates, which provide controllable, well-organized nanoparticles on the surface, for the analysis of a fucose binding lectin AAL. The SERS based assay provides fast (<10 s), and reproducible (<5% variation) detection.
authors.affiliation: Chemistry Department, Pennsylvania State University, University Park, PA 16802, USA.
authors.forename: M A
authors.initials: MA
authors.lastname: Santiago-Cordoba
authors.forename: P R
authors.initials: PR
authors.lastname: Romano
authors.forename: A
authors.initials: A
authors.lastname: MacKay
authors.forename: M C
authors.initials: MC
authors.lastname: Demirel
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis*; D006031:Glycosylation; D006801:Humans; D008113:Liver Neoplasms / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D013059:Spectrum Analysis, Raman
chemical_list: D014408:Biomarkers, Tumor