title: Breath analysis during one-lung ventilation in cancer patients.
abstract: Noninvasive breath analysis may provide valuable information for cancer recognition if disease-specific volatile biomarkers could be identified. In order to compare nondiseased and diseased tissue in vivo, this study took advantage of the special circumstances of one-lung ventilation (OLV) during lung-surgery. 15 cancer patients undergoing lung resection with OLV were enrolled. From each patient, alveolar breath samples were taken separately from healthy and diseased lungs before and after tumour resection. Volatile substances were pre-concentrated by means of solid-phase microextraction, and were separated, identified and quantified by means of gas chromatography-mass spectrometry. Different classes of volatile substances could be identified according to their concentration profiles. Due to prolonged fasting and activation of lipolysis, concentrations of endogenous acetone significantly increased during surgery. Exogenous substances, such as benzene or cyclohexanone, showed typical washout exhalation kinetics. Exhaled concentrations of potentially tumour associated substances, such as butane or pentane, were different for nondiseased and diseased lungs and decreased significantly after surgery. Separate analysis of volatile substances exhaled from healthy and diseased lungs in the same patient, together with thorough consideration of substance origins and exhalation kinetics offers unique opportunities of biomarker recognition and evaluation.
authors.affiliation: Department of Anaesthesiology and Intensive Care Medicine, University Rostock, Schillingallee 35, 18057 Rostock, Germany. sabine.kischkel@uni-rostock.de
authors.forename: Sabine
authors.initials: S
authors.lastname: Kischkel
authors.forename: Wolfram
authors.initials: W
authors.lastname: Miekisch
authors.forename: Patricia
authors.initials: P
authors.lastname: Fuchs
authors.forename: Jochen K
authors.initials: JK
authors.lastname: Schubert
mesh_terms: D000096:Acetone / Q000032:analysis; D000368:Aged; D001554:Benzene / Q000032:analysis; D014408:Biomarkers, Tumor / Q000032:analysis; D001944:Breath Tests; D002073:Butanes / Q000032:analysis; D003512:Cyclohexanones / Q000032:analysis; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D061810:One-Lung Ventilation; D010420:Pentanes / Q000032:analysis; D052617:Solid Phase Microextraction; D055549:Volatile Organic Compounds / Q000032:analysis
chemical_list: D014408:Biomarkers, Tumor; D002073:Butanes; D003512:Cyclohexanones; D010420:Pentanes; D055549:Volatile Organic Compounds; D000096:Acetone; C033353:pentane; C036468:cyclohexanone; C046888:butane; D001554:Benzene