title: NCE102 homologue in Aspergillus fumigatus is required for normal sporulation, not hyphal growth or pathogenesis.
abstract: In Saccharomyces cerevisiae, Nce102 encodes a 173 amino acid transmembrane protein, which acts as a key player in eisosome assembly and plasma membrane organization. Here, we describe the characterization of Nce102 homologue in the human pathogen, Aspergillus fumigatus. Our results demonstrated that AfuNce102 is continuously expressed during fungal growth. In addition, microscopic examination of an AfuNce102-GFP-expressing transformant confirmed the localization of the fusion protein to the endoplasmic reticulum with higher density fluorescence at the tip of the mycelium. During conidiogenesis, the protein was localized to the conidiophores and the conidia. Abnormal conidiation of AfuNce102 deletion mutant suggests a potential role for AfuNce102 in sporulation process.
authors.affiliation: Fungal Biotechnology Group, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran. khalajs@pasteur.ac.ir
authors.forename: Vahid
authors.initials: V
authors.lastname: Khalaj
authors.forename: Mohammad
authors.initials: M
authors.lastname: Azizi
authors.forename: Somayeh
authors.initials: S
authors.lastname: Enayati
authors.forename: Dorsa
authors.initials: D
authors.lastname: Khorasanizadeh
authors.forename: Esmat Mirabzadeh
authors.initials: EM
authors.lastname: Ardakani
mesh_terms: D000818:Animals; D001228:Aspergillosis / Q000382:microbiology / Q000401:mortality; D001232:Aspergillus fumigatus / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D004721:Endoplasmic Reticulum / Q000737:chemistry / Q000378:metabolism; D005260:Female; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D005816:Genetic Complementation Test; D049452:Green Fluorescent Proteins; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008827:Microbiological Phenomena; D009154:Mutation; D025282:Mycelium / Q000737:chemistry / Q000378:metabolism; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013172:Spores, Fungal / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D016019:Survival Analysis
chemical_list: D005656:Fungal Proteins; D008565:Membrane Proteins; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D049452:Green Fluorescent Proteins