title: Simultaneous determination of stilbenes, phenolic acids, flavonoids and anthraquinones in Radix polygoni multiflori by LC-MS/MS.
abstract: A simple and sensitive method was developed for the simultaneous determination of stilbenes, phenolic acid, flavonoids and anthraquinones in Radix polygoni multiflori by liquid chromatography tandem mass spectrometry (LC-MS/MS). The separation was completed on an Eclipse Plus C(18) (50 mm × 3.0 mm, 1.8 μm) column using 0.05% (v/v) formic acid and acetonitrile as mobile phases. The correlation coefficients of all the calibration curves were higher than 0.9990. The recoveries ranged from 95.9% to 106%. Relative standard deviations of intra and inter-day precisions were lower than 6.51%. The validated method was successfully applied to quantify stilbenes, phenolic acid, flavonoids and anthraquinones, which provided a new basis for overall assessment on quality of Radix polygoni multiflori.
authors.affiliation: Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors.forename: Zi-wei
authors.initials: ZW
authors.lastname: Zhu
authors.forename: Jin
authors.initials: J
authors.lastname: Li
authors.forename: Xiu-mei
authors.initials: XM
authors.lastname: Gao
authors.forename: Ernestina
authors.initials: E
authors.lastname: Amponsem
authors.forename: Li-yuan
authors.initials: LY
authors.lastname: Kang
authors.forename: Li-min
authors.initials: LM
authors.lastname: Hu
authors.forename: Bo-li
authors.initials: BL
authors.lastname: Zhang
authors.forename: Yan-xu
authors.initials: YX
authors.lastname: Chang
mesh_terms: D000880:Anthraquinones / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D005419:Flavonoids / Q000032:analysis*; D062385:Hydroxybenzoates / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry*; D015203:Reproducibility of Results; D013267:Stilbenes / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000880:Anthraquinones; D005419:Flavonoids; D062385:Hydroxybenzoates; D013267:Stilbenes; C017616:phenolic acid