title: Revealing stable processing products from ribosome-associated small RNAs by deep-sequencing data analysis.
abstract: The exploration of the non-protein-coding RNA (ncRNA) transcriptome is currently focused on profiling of microRNA expression and detection of novel ncRNA transcription units. However, recent studies suggest that RNA processing can be a multi-layer process leading to the generation of ncRNAs of diverse functions from a single primary transcript. Up to date no methodology has been presented to distinguish stable functional RNA species from rapidly degraded side products of nucleases. Thus the correct assessment of widespread RNA processing events is one of the major obstacles in transcriptome research. Here, we present a novel automated computational pipeline, named APART, providing a complete workflow for the reliable detection of RNA processing products from next-generation-sequencing data. The major features include efficient handling of non-unique reads, detection of novel stable ncRNA transcripts and processing products and annotation of known transcripts based on multiple sources of information. To disclose the potential of APART, we have analyzed a cDNA library derived from small ribosome-associated RNAs in Saccharomyces cerevisiae. By employing the APART pipeline, we were able to detect and confirm by independent experimental methods multiple novel stable RNA molecules differentially processed from well known ncRNAs, like rRNAs, tRNAs or snoRNAs, in a stress-dependent manner.
authors.affiliation: Innsbruck Biocenter, Medical University Innsbruck, Division of Genomics and RNomics, Fritz-Pregl-Strasse 3, 6020 Innsbruck, Austria. marek.zywicki@i-med.ac.at
authors.forename: Marek
authors.initials: M
authors.lastname: Zywicki
authors.forename: Kamilla
authors.initials: K
authors.lastname: Bakowska-Zywicka
authors.forename: Norbert
authors.initials: N
authors.lastname: Polacek
mesh_terms: D020869:Gene Expression Profiling; D015723:Gene Library; D059014:High-Throughput Nucleotide Sequencing; D012323:RNA Processing, Post-Transcriptional; D020871:RNA Stability; D058727:RNA, Small Untranslated / Q000737:chemistry / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D017423:Sequence Analysis, RNA; D012984:Software
chemical_list: D058727:RNA, Small Untranslated