title: [The prediction of immunogenicity of therapeutic proteins].
abstract: Immunogenicity of therapeutic proteins is a nightmare for industrials because induced antibodies can neutralize the therapeutic activity and provoke autoimmune symptoms. It was believed that sequence humanization would be sufficient to tackle these problems but multiple clinical examples now demonstrate that humanization does not suffice to abrogate immune responses. In order to predict immunogenicity of therapeutic proteins, different approaches have been developed, among which the most relevant ones are based on the evaluation of the response of naïve CD4 T lymphocytes specific for therapeutic proteins. Other approaches also exist or are in development. This review is the state of art in the different technologies that are proposed to predict immunogenicity of therapeutic proteins.
authors.affiliation: CEA, Institut de Biologie et de Technologies de Saclay, SIMOPRO, 91191 Gif sur Yvette, France. bernard.maillere@cea.fr
authors.forename: Bernard
authors.initials: B
authors.lastname: Maillère
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Delluc
authors.forename: Gilles
authors.initials: G
authors.lastname: Ravot
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000627:therapeutic use; D061067:Antibodies, Monoclonal, Humanized / Q000276:immunology / Q000627:therapeutic use; D001327:Autoimmune Diseases / Q000209:etiology / Q000517:prevention & control; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002678:Chimera; D004351:Drug Resistance / Q000276:immunology; D004921:Erythropoietin / Q000276:immunology / Q000627:therapeutic use; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007114:Immunization; D007159:Immunologic Tests; D007518:Isoantibodies / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D051379:Mice; D008822:Mice, Transgenic; D018448:Models, Immunological; D011506:Proteins / Q000276:immunology* / Q000627:therapeutic use*; D011994:Recombinant Proteins / Q000276:immunology / Q000627:therapeutic use
chemical_list: D000911:Antibodies, Monoclonal; D061067:Antibodies, Monoclonal, Humanized; D007518:Isoantibodies; D011506:Proteins; D011994:Recombinant Proteins; D004921:Erythropoietin