title: Radioligand binding characterization of neuronal nicotinic acetylcholine receptors.
abstract: Described in this unit are radioligand binding protocols for three neuronal nicotinic acetylcholine receptor (nAChR) subtypes. Detailed descriptions of binding protocols are presented for the two predominant CNS subtypes of nAChRs, α4β2 and α7, as well as the ganglionic α3β4 nAChR. [(3)H]Cytisine is utilized for α4β2 nAChRs, while [(3)H]methyllycaconitine is utilized for α7 nAChRs, both in rat brain. α3β4 nAChRs in IMR-32 cells are labeled with [(3)H]epibatidine. Reference data are presented, as well as commentary on the current state of neuronal nicotinic receptor research.
authors.affiliation: Abbott Laboratories, Abbott Park, Illinois, USA.
authors.forename: David J
authors.initials: DJ
authors.lastname: Anderson
mesh_terms: D000818:Animals; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D002498:Centrifugation / Q000379:methods; D008024:Ligands; D008297:Male; D009474:Neurons / Q000378:metabolism*; D018722:Nicotinic Agonists / Q000494:pharmacology; D011725:Pyridines / Q000494:pharmacology; D011869:Radioligand Assay / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011978:Receptors, Nicotinic / Q000378:metabolism*; D013048:Specimen Handling / Q000379:methods; D064569:alpha7 Nicotinic Acetylcholine Receptor
chemical_list: D019086:Bridged Bicyclo Compounds, Heterocyclic; D008024:Ligands; D018722:Nicotinic Agonists; D011725:Pyridines; D011978:Receptors, Nicotinic; D064569:alpha7 Nicotinic Acetylcholine Receptor; C579165:Chrna7 protein, rat; C082748:epibatidine