title: Measurement of VLA-4/CS-1 and VLA-4/VCAM adhesion inhibition.
abstract: Cell adhesion, a critical early step in the inflammatory process, has increasingly become the target of drug discovery efforts. Described in this unit are techniques for measuring inhibitors of VLA-4-mediated adhesion to either VCAM or the connecting segment (CS-1) of fibronectin.
authors.affiliation: University of Washington, Seattle, Washington, USA.
authors.forename: Christopher
authors.initials: C
authors.lastname: Mehlin
mesh_terms: D002448:Cell Adhesion / Q000502:physiology*; D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D002712:Chlorides / Q000494:pharmacology; D003625:Data Collection; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D039041:Integrin alpha4beta1 / Q000037:antagonists & inhibitors*; D017895:Manganese Compounds / Q000494:pharmacology; D016029:Receptors, Very Late Antigen / Q000502:physiology*; D013048:Specimen Handling / Q000379:methods; D019010:Vascular Cell Adhesion Molecule-1 / Q000502:physiology*
chemical_list: D002712:Chlorides; D005353:Fibronectins; D039041:Integrin alpha4beta1; D017895:Manganese Compounds; D016029:Receptors, Very Late Antigen; D019010:Vascular Cell Adhesion Molecule-1; C025340:manganese chloride