title: Identification and characterization of microsatellite loci in the rush Juncus effusus (Juncaceae).
abstract: OBJECTIVE
  Microsatellite markers were developed for the common wetland plant Juncus effusus to assess levels of within- and among-population genetic diversity.
  
  
  RESULTS
  Using a next-generation sequencing approach we identified new microsatellite loci, from which 23 were further characterized in a large population of J. effusus. Expected heterozygosity ranged from 0 to 0.64 with a mean of 0.407. Inbreeding coefficients (mean = 0.984) were very high, confirming earlier suggestions of an inbred mating system. Cross-amplification was tested in J. conglomeratus, J. inflexus, J. drummondii, and J. filiformis, with best results in the former.
  
  
  CONCLUSIONS
  The described microsatellite markers will be helpful in assisting studies on, e.g., reproduction, taxonomy, and phylogeography in J. effusus and related species.
authors.affiliation: Department of Community Ecology (BZF), Helmholtz Centre for Environmental Research UFZ, Theodor-Lieser-Strasse 4, D-06120 Halle, Germany. stefan.michalski@ufz.de
authors.forename: Stefan G
authors.initials: SG
authors.lastname: Michalski
authors.forename: Walter
authors.initials: W
authors.lastname: Durka
mesh_terms: D002467:Cell Nucleus / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D056426:Genetic Loci; D014644:Genetic Variation; D006579:Heterozygote; D007178:Inbreeding; D019684:Magnoliopsida / Q000235:genetics*; D018895:Microsatellite Repeats; D013045:Species Specificity; D053833:Wetlands
chemical_list: D017931:DNA Primers; D018744:DNA, Plant