title: Applicability of accelerated solvent extraction for synthetic colorants analysis in meat products with ultrahigh performance liquid chromatography-photodiode array detection.
abstract: Accelerated solvent extraction (ASE) coupled with ultrahigh performance liquid chromatography (UHPLC) with photodiode array detection (PDA) has been used for the quantitative determination of synthetic colorants in meat products. Samples were extracted with ethanol-water-ammonia with a ratio of 75:24:1 (v/v/v) using ASE instrument at 85°C. As a result, all the colorants in meat products were separated using an optimized gradient elution within 3.5 min. Detection and quantification limits of synthetic colorants were in the ranges of 0.01-0.02 mg kg(-1) and 0.05 mg kg(-1), respectively. The intra-day and inter-day precision of the synthetic colorants were ranged between 1.7% (E123) to 5.2% (E124) and 3.2% (E124) to 6.0% (E129), respectively. The intra-day and inter-day recoveries of the synthetic colorants were ranged between 76.9% (E124) to 84.9% (E102) and 76.3% (E124) to 84.3% (E127), respectively. The method has been applied for the determination of seven synthetic colorants in meat products.
authors.affiliation: Jiangxi Academy of Agricultural Sciences Key Laboratory of Quality and Safety of Agricultural Products, Agricultural Product Quality Safety and Standards Institute, Jiangxi Academy of Agricultural Sciences, Nanchang, PR China.
authors.forename: Qie Gen
authors.initials: QG
authors.lastname: Liao
authors.forename: Wei Hong
authors.initials: WH
authors.lastname: Li
authors.forename: Lin Guang
authors.initials: LG
authors.lastname: Luo
mesh_terms: D000641:Ammonia / Q000737:chemistry; D005591:Chemical Fractionation; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol / Q000737:chemistry; D005505:Food Coloring Agents / Q000032:analysis* / Q000302:isolation & purification; D008461:Meat Products / Q000032:analysis*; D012997:Solvents / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D005505:Food Coloring Agents; D012997:Solvents; D014867:Water; D000431:Ethanol; D000641:Ammonia