title: A fast, sensitive, and high throughput method for the determination of cefuroxime lysine in dog plasma by UPLC-MS/MS.
abstract: In order to investigate the preclinical pharmacokinetics of cefuroxime lysine, a fast, sensitive and high throughput UPLC-ESI-MS/MS method has been developed and validated for the quantitative determination of cefuroxime in dog plasma. Cefuroxime and IS phenacetin were extracted from plasma samples by PPT or LLE procedure, and then separated on an ACQUITY UPLC™ BEH C(18) column with an isocratic elution of acetonitrile-0.1% formic acid in 10mM ammonium acetate (40:60, v/v). MRM using the fragmentation transitions of m/z 442 → 364 and 180 → 110 in positive ESI mode was performed to quantify cefuroxime and IS, respectively. The calibration curves were linear over the concentration range of 2-400 μg/ml for PPT and 0.01-5 μg/ml for LLE. The LLOQ was 0.01 μg/ml. The intra- and inter-day precisions in all samples were no more than 8.1%, while the accuracy was within ± 6.2% of nominal values. The method was successfully applied to the evaluation of pharmacokinetic parameters of cefuroxime lysine in beagle dogs.
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Longshan
authors.initials: L
authors.lastname: Zhao
authors.forename: Yunli
authors.initials: Y
authors.lastname: Zhao
authors.forename: Qing
authors.initials: Q
authors.lastname: Li
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Chen
authors.forename: Feng
authors.initials: F
authors.lastname: Xiao
authors.forename: Bosai
authors.initials: B
authors.lastname: He
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.forename: Kaishun
authors.initials: K
authors.lastname: Bi
mesh_terms: D000085:Acetates / Q000737:chemistry; D000097:Acetonitriles / Q000737:chemistry; D000818:Animals; D000900:Anti-Bacterial Agents / Q000097:blood* / Q000493:pharmacokinetics; D002138:Calibration; D002444:Cefuroxime / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D004355:Drug Stability; D005260:Female; D005561:Formates; D057166:High-Throughput Screening Assays; D059625:Liquid-Liquid Extraction; D008297:Male; D012015:Reference Standards; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry
chemical_list: D000085:Acetates; D000097:Acetonitriles; D000900:Anti-Bacterial Agents; D005561:Formates; C030544:formic acid; C027301:cefuroxime lysine; D002444:Cefuroxime; C018824:ammonium acetate; C032159:acetonitrile