title: Identification of promoter region methylation patterns of MGMT, CDKN2A, GSTP1, and THBS1 genes in intracranial meningioma patients.
abstract: BACKGROUND
  Cytogenetic, molecular and epigenetic changes are all known to take place in the pathogenesis of meningiomas. In this study, we aimed at investing methylation of MGMT (DNA repair), CDKN2A (cell cycle control), GSTP1 (detoxification), and THBS1 (angiogenesis inhibitor) genes, which are known to be unmethylated in normal tissue, in meningioma samples.
  
  
  METHODS
  Methylation specific polymerase chain reaction was used to study promoter regions methylation of genes in 36 patient samples.
  
  
  RESULTS
  Methylation in promoter regions of MGMT, CDKN2A, GSTP1, and THBS1 genes were found in 11.1%, 8.3%, 2.8%, and 0% of the cases, respectively. About 19.4% of cases revealed promoter methylation of at least a single gene, whereas only 2.8% of cases revealed methylation of more than one gene. Based on their World Health Organization 2007 grade; 6.3% of grade I cases, 35.3% of grade II cases, and 33.3% of grade III cases showed hypermethylation in the promoter regions of the genes studied. No statistically significant relation was found between promoter zone methylation and factors such as age, sex, histopathology, grade, or recurrence.
  
  
  CONCLUSIONS
  Further research on promoter zone methylation will help expose the methylation profile and pathogenesis of meningiomas, which will consequently guide to a deeper understanding of the pathogenesis of the disease, thus ensuring a better understanding of the prognosis and considering novel treatment options.
authors.affiliation: Department of Neurosurgery, Baskent University, Ankara, Turkey.
authors.forename: Fatih
authors.initials: F
authors.lastname: Aydemir
authors.forename: Erkan
authors.initials: E
authors.lastname: Yurtcu
authors.forename: Tugce Bulakbasi
authors.initials: TB
authors.lastname: Balci
authors.forename: Feride Iffet
authors.initials: FI
authors.lastname: Sahin
authors.forename: Salih
authors.initials: S
authors.lastname: Gulsen
authors.forename: Nur
authors.initials: N
authors.lastname: Altinors
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019175:DNA Methylation; D015254:DNA Modification Methylases / Q000235:genetics*; D045643:DNA Repair Enzymes / Q000235:genetics*; D044127:Epigenesis, Genetic; D005260:Female; D019942:Genes, p16; D051549:Glutathione S-Transferase pi / Q000235:genetics*; D006801:Humans; D008297:Male; D008579:Meningioma / Q000235:genetics* / Q000473:pathology; D008875:Middle Aged; D011401:Promoter Regions, Genetic / Q000235:genetics*; D019699:Thrombospondins / Q000235:genetics; D025521:Tumor Suppressor Proteins / Q000235:genetics*; D055815:Young Adult; D000099262:Thrombospondin 2
chemical_list: D015254:DNA Modification Methylases; D045643:DNA Repair Enzymes; D051549:Glutathione S-Transferase pi; D019699:Thrombospondins; D025521:Tumor Suppressor Proteins; D000099262:Thrombospondin 2; C515491:MGMT protein, human; C496556:GSTP1 protein, human