title: [Mechanisms of interactions between glycine- and GABA-mediated responses in spinal cord neurons].
abstract: The interactions of GABA- and glycine-mediated responses have been analyzed, the whole cell patch-clamp method being used. The response induced by co-application of glycine and GABA was a lesser one than the sum of responses induced by applying two transmitters separately. The molecular mechanisms underlying this effect have been determined. Due to applications of high concentrations of neurotransmitters it was revealed that GABA could activate glycine receptors in frog spinal motoneurons with relatively high efficiency (EC50 = 1.2 mM). The sequential application of neurotransmitters showed that even a single application of glycine could significantly boost the "run-down" of the GABA-mediated current, suggesting that there was a strong phosphorylation-dependent mechanism of GABAa-receptors inhibition. These mechanisms are likely to take place in frog spinal motoneurons when GABA and glycine are co-released from the same synaptic terminal.
authors.forename: D B
authors.initials: DB
authors.lastname: Amakhin
authors.forename: N P
authors.initials: NP
authors.lastname: Veselkin
mesh_terms: D000818:Animals; D004594:Electrophysiology; D005998:Glycine / Q000494:pharmacology*; D008564:Membrane Potentials / Q000187:drug effects; D009046:Motor Neurons / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D018408:Patch-Clamp Techniques; D010766:Phosphorylation; D011896:Rana temporaria / Q000378:metabolism / Q000502:physiology*; D011963:Receptors, GABA-A / Q000187:drug effects / Q000378:metabolism*; D018009:Receptors, Glycine / Q000037:antagonists & inhibitors / Q000187:drug effects / Q000378:metabolism*; D013116:Spinal Cord / Q000187:drug effects / Q000502:physiology*; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology*
chemical_list: D011963:Receptors, GABA-A; D018009:Receptors, Glycine; D005680:gamma-Aminobutyric Acid; D005998:Glycine