title: Mouse models of human bladder cancer as a tool for drug discovery.
abstract: Muscle-invasive bladder cancer is a deadly condition in dire need of effective new treatments. This unit contains a description of mouse models suitable for the evaluation of potential new therapies. Included is a genetically engineered mouse model of bladder cancer generated by the delivery of an adenovirus expressing Cre recombinase into the bladder lumen. Also described is an orthotopic mouse model created by the instillation of human bladder tumor cells into the bladder lumen of immune deficient mice. Protocols are also provided on the use of these models for the preclinical evaluation of new chemical entities, with mTOR inhibitors shown as an example.
authors.affiliation: Department of Urology, Columbia University Medical Center, Herbert Irving Comprehensive Cancer Center, New York, New York, USA.
authors.forename: Catherine
authors.initials: C
authors.lastname: Seager
authors.forename: Anna M
authors.initials: AM
authors.lastname: Puzio-Kuter
authors.forename: Carlos
authors.initials: C
authors.lastname: Cordon-Cardo
authors.forename: James
authors.initials: J
authors.lastname: McKiernan
authors.forename: Cory
authors.initials: C
authors.lastname: Abate-Shen
mesh_terms: D000256:Adenoviridae; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D055808:Drug Discovery / Q000379:methods*; D005260:Female; D005818:Genetic Engineering / Q000379:methods; D005822:Genetic Vectors; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D013048:Specimen Handling / Q000379:methods; D001749:Urinary Bladder Neoplasms / Q000188:drug therapy*
chemical_list: D000970:Antineoplastic Agents