title: Upregulated expression of ADAM12 is associated with progression of oral             squamous cell carcinoma.
abstract: ADAMs are a disintegrin and metalloproteinase family of membrane-associated             metalloproteinases characterized by their multidomain structure, and have been             reported to be associated with various malignant tumors. The aim of this study             was to identify crucial members of the ADAM family in oral squamous cell carcinoma             (OSCC), and to reveal their biological function and clinical significance. To             clarify whether ADAM family genes are involved in OSCC, changes in the expression             profile were investigated by real-time quantitative reverse transcriptase-polymerase             chain reaction (qRT-PCR) analysis and immunohistochemical analysis. Functional             analysis was performed by comparing cellular proliferation of siADAM-transfected             cell lines and parental cell lines. Real-time qRT-PCR analysis identified significantly             upregulated expression of ADAM12 in OSCC-derived cell lines. This was validated             in OSCC samples using real-time qRT-PCR and immuno-histochemical staining. ADAM12             expression was correlated with TNM classification; significantly greater expression             of ADAM12 was observed in tumors with higher T classification and more advanced             stages. Moreover, siADAM12-transfected cells showed both a suppressed proliferation             rate and increased transforming growth factor (TGF)-β3 expression. Our data indicate             that ADAM12 is overexpressed in OSCC and might accelerate cellular proliferation.             Its function may be associated with TGF-β signaling. This study suggests that             controlling the expression or activity of ADAM12 could be a useful strategy in             the development of an effective cure for OSCC.
authors.affiliation: School of Medicine, Chiba University, Chiba 260-8670, Japan.
authors.forename: Erika
authors.initials: E
authors.lastname: Uehara
authors.forename: Masashi
authors.initials: M
authors.lastname: Shiiba
authors.forename: Keiji
authors.initials: K
authors.lastname: Shinozuka
authors.forename: Kengo
authors.initials: K
authors.lastname: Saito
authors.forename: Yukinao
authors.initials: Y
authors.lastname: Kouzu
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Koike
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kasamatsu
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Sakamoto
authors.forename: Katsunori
authors.initials: K
authors.lastname: Ogawara
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Uzawa
authors.forename: Hideki
authors.initials: H
authors.lastname: Tanzawa
mesh_terms: D051722:ADAM Proteins / Q000235:genetics / Q000378:metabolism*; D000072199:ADAM12 Protein; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D016009:Chi-Square Distribution; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D009367:Neoplasm Staging; D011379:Prognosis; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014162:Transfection; D053782:Transforming Growth Factor beta3 / Q000378:metabolism; D047368:Tumor Burden; D015854:Up-Regulation
chemical_list: D008565:Membrane Proteins; D012333:RNA, Messenger; C509044:TGFB3 protein, human; D053782:Transforming Growth Factor beta3; D051722:ADAM Proteins; D000072199:ADAM12 Protein; C000606480:ADAM12 protein, human