title: Genotyping of Human parvovirus B19 among Brazilian patients with hemoglobinopathies.
abstract: Human parvovirus B19 (B19V) infection can be a life-threatening condition among patients with hereditary (chronic) hemolytic anemias. Our objective was to characterize the infection molecularly among patients with sickle cell disease and thalassemia. Forty-seven patients (37 with sickle cell disease, and 10 with β-thalassemia major) as well as 47 healthy blood donors were examined for B19V infection by anti-B19V IgG enzyme immunoassay, quantitative PCR, which detects all B19V genotypes, and DNA sequencing. B19V viremia was documented in nine patients (19.1%) as two displayed acute infection and the rest had a low titre viremia (mean 3.4 × 10(4) copies/mL). All donors were negative for B19V DNA. Anti-B19V IgG was detected in 55.3% of the patients and 57.4% among the donors. Based on partial NS1 fragments, all patient isolates were classified as genotype 1 and subgenotype 1A. The evolutionary events of the examined partial NS1 gene sequence were associated with a lack of positive selection. The quantification of all B19V genotypes by a single hydrolytic probe is a technically useful method, but it is difficult to establish relationships between B19V sequence characteristics and infection outcome.
authors.affiliation: Regional Blood Center of Ribeirão Preto-FUNDHERP, Faculty of Medicine in Ribeirão Preto-FMRP, University of São Paulo-USP, 2051 Tenente Catão Roxo Str., Ribeirão Preto, São Paulo, Brazil. svetlyosf@yahoo.com
authors.forename: Svetoslav Nanev
authors.initials: SN
authors.lastname: Slavov
authors.forename: Simone
authors.initials: S
authors.lastname: Kashima
authors.forename: Ana Cristina
authors.initials: AC
authors.lastname: Silva-Pinto
authors.forename: Dimas Tadeu
authors.initials: DT
authors.lastname: Covas
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000755:Anemia, Sickle Cell / Q000453:epidemiology / Q000235:genetics / Q000821:virology*; D001782:Blood Donors; D001938:Brazil; D002648:Child; D002675:Child, Preschool; D004247:DNA / Q000235:genetics; D004279:DNA, Viral / Q000097:blood / Q000235:genetics; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D010322:Parvoviridae Infections / Q000175:diagnosis / Q000453:epidemiology / Q000235:genetics / Q000821:virology; D016732:Parvovirus B19, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013789:Thalassemia / Q000235:genetics; D014766:Viremia / Q000175:diagnosis / Q000235:genetics; D055815:Young Adult; D017086:beta-Thalassemia / Q000453:epidemiology / Q000235:genetics / Q000821:virology*
chemical_list: D004279:DNA, Viral; D004247:DNA