title: Amelioration of alcohol-induced hepatotoxicity by the administration of ethanolic extract of Sida cordifolia Linn.
abstract: Sida cordifolia Linn. (Malvaceae) is a plant used in folk medicine for the treatment of the inflammation of oral mucosa, asthmatic bronchitis, nasal congestion and rheumatism. We studied the hepatoprotective activity of 50 % ethanolic extract of S. cordifolia Linn. against alcohol intoxication. The duration of the experiment was 90 d. The substantially elevated levels of toxicity markers such as alanine aminotransferase, aspartate aminotransferase and γ-glutamyl transferase due to the alcohol treatment were significantly lowered in the extract-treated groups. The activity of antioxidant enzymes and glutathione content, which was lowered due to alcohol toxicity, was increased to a near-normal level in the co-administered group. Lipid peroxidation products, protein carbonyls, total collagen and hydroxyproline, which were increased in the alcohol-treated group, were reduced in the co-administered group. The mRNA levels of cytochrome P450 2E1, NF-κB, TNF-α and transforming growth factor-β1 were found to be increased in the alcohol-treated rats, and their expressions were found to be decreased in the co-administered group. These observations were reinforced by histopathological analysis. Thus, the present study clearly indicates that 50 % ethanolic extract of the roots of S. cordifolia Linn. has a potent hepatoprotective action against alcohol-induced toxicity, which was mediated by lowering oxidative stress and by down-regulating the transcription factors.
authors.affiliation: Department of Biochemistry, University of Kerala, Kariavattom, Thiruvananthapuram, Kerala, India.
authors.forename: S
authors.initials: S
authors.lastname: Rejitha
authors.forename: P
authors.initials: P
authors.lastname: Prathibha
authors.forename: M
authors.initials: M
authors.lastname: Indira
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000627:therapeutic use*; D015415:Biomarkers / Q000378:metabolism; D019392:Cytochrome P-450 CYP2E1 / Q000235:genetics / Q000378:metabolism; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D019587:Dietary Supplements; D015536:Down-Regulation; D000431:Ethanol / Q000737:chemistry; D005235:Fatty Liver, Alcoholic / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology / Q000517:prevention & control*; D048550:Hepatic Insufficiency / Q000209:etiology / Q000517:prevention & control*; D015227:Lipid Peroxidation; D008099:Liver / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D008297:Male; D019660:Malvaceae / Q000737:chemistry*; D008515:Medicine, Ayurvedic; D018384:Oxidative Stress; D010936:Plant Extracts / Q000302:isolation & purification / Q000627:therapeutic use*; D018517:Plant Roots / Q000737:chemistry*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012997:Solvents / Q000737:chemistry
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D016207:Cytokines; D010936:Plant Extracts; D012333:RNA, Messenger; D012997:Solvents; D000431:Ethanol; D019392:Cytochrome P-450 CYP2E1