title: The carboxyl-terminal region of Crtac1B/LOTUS acts as a functional domain in endogenous antagonism to Nogo receptor-1.
abstract: Myelin-derived axon growth inhibitors, such as Nogo, bind to Nogo receptor-1 (NgR1) and thereby limit the action of axonal regeneration after injury in the adult central nervous system. Recently, we have found that cartilage acidic protein-1B (Crtac1B)/lateral olfactory tract usher substance (LOTUS) binds to NgR1 and functions as an endogenous NgR1 antagonist. To examine the functional domain of LOTUS in the antagonism to NgR1, analysis using the deletion mutants of LOTUS was performed and revealed that the carboxyl-terminal region (UA/EC domain) of LOTUS bound to NgR1. The UA/EC fragment of LOTUS overexpressed together with NgR1 in COS7 cells abolished the binding of Nogo66 to NgR1. Overexpression of the UA/EC fragment in cultured chick dorsal root ganglion neurons suppressed Nogo66-induced growth cone collapse. These findings suggest that the UA/EC region is a functional domain of LOTUS serving for an antagonistic action to NgR1.
authors.affiliation: Department of Molecular Pharmacology and Neurobiology, Yokohama City University Graduate School of Medicine, Fuku-ura 3-9, Kanazawa Ward, Yokohama 236-0004, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Kurihara
authors.forename: Yuko
authors.initials: Y
authors.lastname: Arie
authors.forename: Masumi
authors.initials: M
authors.lastname: Iketani
authors.forename: Hiromu
authors.initials: H
authors.lastname: Ito
authors.forename: Kuniyuki
authors.initials: K
authors.lastname: Nishiyama
authors.forename: Yasufumi
authors.initials: Y
authors.lastname: Sato
authors.forename: Fumio
authors.initials: F
authors.lastname: Nakamura
authors.forename: Nobuhisa
authors.initials: N
authors.lastname: Mizuki
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Goshima
authors.forename: Kohtaro
authors.initials: K
authors.lastname: Takei
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D002642:Chick Embryo; D058851:GPI-Linked Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D020439:Growth Cones / Q000378:metabolism; D051379:Mice; D009185:Myelin Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000070816:Nogo Receptor 1; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011956:Receptors, Cell Surface / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D000199:Actins; D002135:Calcium-Binding Proteins; C560339:Crtac1 protein, mouse; D058851:GPI-Linked Proteins; D009185:Myelin Proteins; D000070816:Nogo Receptor 1; D011956:Receptors, Cell Surface; C419790:Rtn4r protein, mouse