title: Induction of specific Th1 responses and suppression of IgE antibody formation by vaccination with plasmid DNA encoding Cyn d 1.
abstract: BACKGROUND
  DNA vaccines encoding allergens have been developed to prevent or to treat specific IgE responses.
  
  
  OBJECTIVE
  To evaluate the potential preventive and therapeutic effect of DNA vaccines encoding Cyn d 1 alone or combined with different adjuvants on specific allergies.
  
  
  METHODS
  Recombinant plasmid Cyn d 1 (pCyn d 1) was constructed by insertion of Cyn d 1 cDNA into the vector pcDNA3. BALB/c mice were injected with pCyn d 1 alone or plus adjuvants such as bupivacaine, bestatin, liposome, or CpG. Control mice were treated with pcDNA3 or PBS. They were boosted 3 weeks later and then sensitized twice with recombinant Cyn d 1 and alum. Their serum antibody responses and cytokine profiles of spleen cells were studied. Adoptive transfer of spleen cells of pCyn d 1-vaccinated mice was also performed.
  
  
  RESULTS
  Vaccination of mice with pCyn d 1 induced Th1 responses characterized by IgG2a responses and spleen cell secretion of interferon-γ. Vaccination with pCyn d 1 not only prevented the induction of specific IgE responses but also suppressed ongoing IgE responses. The mice receiving untreated, CD4+- or CD8+-depleted spleen cells from pCyn d 1-vaccinated mice all had suppression of IgE responses.
  
  
  CONCLUSIONS
  This study confirms the prophylactic and therapeutic effects of DNA vaccines encoding Bermuda grass pollen allergen Cyn d 1 on specific IgE responses. Both CD4+ and CD8+ T cells are crucial for the immunomodulatory effect of pCyn d 1 on specific IgE responses.
authors.affiliation: Department of Pediatrics, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC.
authors.forename: Ching-Feng
authors.initials: CF
authors.lastname: Huang
authors.forename: Chia-Hsiang
authors.initials: CH
authors.lastname: Chu
authors.forename: Chia-Chao
authors.initials: CC
authors.lastname: Wu
authors.forename: Zo-Nan
authors.initials: ZN
authors.lastname: Chang
authors.forename: Fung-Lin
authors.initials: FL
authors.lastname: Chue
authors.forename: Ho-Jen
authors.initials: HJ
authors.lastname: Peng
mesh_terms: D000276:Adjuvants, Immunologic; D019264:Adoptive Transfer / Q000379:methods; D000818:Animals; D052179:Antigens, Plant / Q000235:genetics* / Q000276:immunology*; D002045:Bupivacaine / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D018899:CpG Islands; D016207:Cytokines / Q000096:biosynthesis / Q000276:immunology; D005260:Female; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000276:immunology*; D007930:Leucine / Q000031:analogs & derivatives / Q000276:immunology; D008081:Liposomes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D014611:Vaccination; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D052179:Antigens, Plant; C550205:Cyn d 1 allergen, Bermuda grass; D016207:Cytokines; D007074:Immunoglobulin G; D008081:Liposomes; D019444:Vaccines, DNA; D007073:Immunoglobulin E; D007930:Leucine; C012211:ubenimex; D002045:Bupivacaine