title: Molecular and functional interactions of cat APOBEC3 and feline foamy and immunodeficiency virus proteins: different ways to counteract host-encoded restriction.
abstract: Defined host-encoded feline APOBEC3 (feA3) cytidine deaminases efficiently restrict the replication and spread of exogenous retroviruses like Feline Immunodeficiency Virus (FIV) and Feline Foamy Virus (FFV) which developed different feA3 counter-acting strategies. Here we characterize the molecular interaction of FFV proteins with the diverse feA3 proteins. The FFV accessory protein Bet is the virus-encoded defense factor which is shown here to bind all feA3 proteins independent of whether they restrict FFV, a feature shared with FIV Vif that induces degradation of all feA3s including those that do not inactivate FIV. In contrast, only some feA3 proteins bind to FFV Gag, a pattern that in part reflects the restriction pattern detected. Additionally, one-domain feA3 proteins can homo- and hetero-dimerize in vitro, but a trans-dominant phenotype of any of the low-activity feA3 forms on FFV restriction by one of the highly-active feA3Z2 proteins was not detectable.
authors.affiliation: German Cancer Research Center (DKFZ), Research Program Infection and Cancer, Heidelberg, Germany.
authors.forename: Sarah
authors.initials: S
authors.lastname: Chareza
authors.forename: Dragana
authors.initials: D
authors.lastname: Slavkovic Lukic
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Ann-Mareen
authors.initials: AM
authors.lastname: Räthe
authors.forename: Carsten
authors.initials: C
authors.lastname: Münk
authors.forename: Elisa
authors.initials: E
authors.lastname: Zabogli
authors.forename: Mauro
authors.initials: M
authors.lastname: Pistello
authors.forename: Martin
authors.initials: M
authors.lastname: Löchelt
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000201:enzymology* / Q000235:genetics / Q000821:virology; D002415:Cats; D002460:Cell Line; D003564:Cytidine Deaminase / Q000235:genetics / Q000378:metabolism*; D016181:Feline Acquired Immunodeficiency Syndrome / Q000201:enzymology* / Q000235:genetics / Q000821:virology; D054884:Host-Pathogen Interactions; D016089:Immunodeficiency Virus, Feline / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D012192:Retroviridae Infections / Q000201:enzymology / Q000662:veterinary* / Q000821:virology; D016092:Spumavirus / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D014764:Viral Proteins; D003564:Cytidine Deaminase