title: Neuroprotective effect of phlorotannin isolated from Ishige okamurae against H₂O₂ -induced oxidative stress in murine hippocampal neuronal cells, HT22.
abstract: The present study is designed to investigate the neuroprotective effect of a kind of phlorotannins, diphlorethohydroxycarmalol (DPHC) isolated from Ishige okamurae against hydrogen peroxide (H(2)O(2))-induced oxidative stress in murine hippocampal neuronal cells, HT22. H(2)O(2) treatment induced neurotoxicity, whereas DPHC prevented cells from H(2)O(2)-induced damage then restoring cell viability was significantly increased. DPHC slightly reduced the expression of Bax induced by H(2)O(2) but recovered the expression of Bcl-xL as well as caspase-9 and -3 mediated PARP cleavage by H(2)O(2). Intracellular reactive oxygen species (ROS) and lipid peroxidation was overproduced as the result of the addition of H(2)O(2); however, these ROS generations and lipid peroxidation were effectively inhibited by addition of DPHC in a dose-dependent manner. Moreover, DPHC suppressed the elevation of H(2)O(2)-induced Ca(2+) release. These findings indicate that DPHC has neuroprotective effects against H(2)O(2)-induced damage in neuronal cells, and that an inhibitory effect on ROS production may contribute to the underlying mechanisms.
authors.affiliation: Marine Biology and Living Resources Research Department, Korea Ocean Research and Development Institute, Ansan, 426-744, Republic of Korea.
authors.forename: Soo-Jin
authors.initials: SJ
authors.lastname: Heo
authors.forename: Seon-Heui
authors.initials: SH
authors.lastname: Cha
authors.forename: Kil-Nam
authors.initials: KN
authors.lastname: Kim
authors.forename: Seung-Hong
authors.initials: SH
authors.lastname: Lee
authors.forename: Ginnae
authors.initials: G
authors.lastname: Ahn
authors.forename: Do-Hyung
authors.initials: DH
authors.lastname: Kang
authors.forename: Chulhong
authors.initials: C
authors.lastname: Oh
authors.forename: Young-Ung
authors.initials: YU
authors.lastname: Choi
authors.forename: Abu
authors.initials: A
authors.lastname: Affan
authors.forename: Daekyung
authors.initials: D
authors.lastname: Kim
authors.forename: You-Jin
authors.initials: YJ
authors.lastname: Jeon
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival; D004305:Dose-Response Relationship, Drug; D006575:Heterocyclic Compounds, 3-Ring / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006624:Hippocampus / Q000166:cytology; D006861:Hydrogen Peroxide / Q000009:adverse effects* / Q000378:metabolism; D015227:Lipid Peroxidation; D051379:Mice; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D018696:Neuroprotective Agents / Q000008:administration & dosage / Q000494:pharmacology*; D018384:Oxidative Stress; D000459:Phaeophyceae / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology
chemical_list: D006575:Heterocyclic Compounds, 3-Ring; D018696:Neuroprotective Agents; D010936:Plant Extracts; C513811:diphlorethohydroxycarmalol; D006861:Hydrogen Peroxide; D002118:Calcium