title: Anti-inflammatory effects of β-sitosterol-β-D-glucoside from Trachelospermum jasminoides (Apocynaceae) in lipopolysaccharide-stimulated RAW 264.7 murine macrophages.
abstract: Trachelospermum jasminoides (Apocynaceae) has pharmacological effects that include anti-inflammatory, anti-bacterial and anti-viral activities, which have been observed from various studies. Of these pharmacological effects, the anti-inflammatory capacity of compounds from T. jasminoides is not yet known exactly. In this study, we investigated the compound that can be used for the suppression of lipopolysacchaide (LPS) stimulated inflammatory responses in macrophages among the five isolated compounds. β-sitosterol-β-D-glucoside (1) was found to reduce nitric oxide (NO) production from LPS-induced RAW 264.7 cells the most. In addition, compound 1 strongly inhibited the interleukin 6 (IL-6) activities of stimulated macrophages. Treatment of RAW 264.7 cells with compound 1 reduced secretion of inflammatory elements including tumour necrosis factor - alpha (TNF-α) and interleukin 1 beta (IL-1β). Thus, compound 1 may be a useful candidate for the development of new drugs to treat endotoxemia and inflammation accompanied by the overproduction of NO.
authors.affiliation: Life Science RD Center, Sinil Pharmaceutical Co., Ltd., Chungju, Korea.
authors.forename: Ji Na
authors.initials: JN
authors.lastname: Choi
authors.forename: Yun-Hyeok
authors.initials: YH
authors.lastname: Choi
authors.forename: Jeong-Min
authors.initials: JM
authors.lastname: Lee
authors.forename: Ill Chan
authors.initials: IC
authors.lastname: Noh
authors.forename: Jae Won
authors.initials: JW
authors.lastname: Park
authors.forename: Wahn Soo
authors.initials: WS
authors.lastname: Choi
authors.forename: Jung Ho
authors.initials: JH
authors.lastname: Choi
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D029062:Apocynaceae / Q000737:chemistry*; D002460:Cell Line; D053583:Interleukin-1beta / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008070:Lipopolysaccharides; D008264:Macrophages / Q000187:drug effects*; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D012855:Sitosterols / Q000302:isolation & purification / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D053583:Interleukin-1beta; D015850:Interleukin-6; D008070:Lipopolysaccharides; D012855:Sitosterols; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; C011015:lyoniside