title: TRAPPC9 mediates the interaction between p150 and COPII vesicles at the target membrane.
abstract: BACKGROUND
  The transport of endoplasmic reticulum (ER)-derived COPII vesicles toward the ER-Golgi intermediate compartment (ERGIC) requires cytoplasmic dynein and is dependent on microtubules. p150(Glued), a subunit of dynactin, has been implicated in the transport of COPII vesicles via its interaction with COPII coat components Sec23 and Sec24. However, whether and how COPII vesicle tether, TRAPP (Transport protein particle), plays a role in the interaction between COPII vesicles and microtubules is currently unknown.
  
  
  RESULTS
  We address the functional relationship between COPII tether TRAPP and dynactin. Overexpressed TRAPP subunits interfered with microtubule architecture by competing p150(Glued) away from the MTOC. TRAPP subunit TRAPPC9 bound directly to p150(Glued) via the same carboxyl terminal domain of p150(Glued) that binds Sec23 and Sec24. TRAPPC9 also inhibited the interaction between p150(Glued) and Sec23/Sec24 both in vitro and in vivo, suggesting that TRAPPC9 serves to uncouple p150(Glued) from the COPII coat, and to relay the vesicle-dynactin interaction at the target membrane.
  
  
  CONCLUSIONS
  These findings provide a new perspective on the function of TRAPP as an adaptor between the ERGIC membrane and dynactin. By preserving the connection between dynactin and the tethered and/or fused vesicles, TRAPP allows nascent ERGIC to continue the movement along the microtubules as they mature into the cis-Golgi.
authors.affiliation: School of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong SAR, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Zong
authors.forename: Ayano
authors.initials: A
authors.lastname: Satoh
authors.forename: Mei Kuen
authors.initials: MK
authors.lastname: Yu
authors.forename: Ka Yu
authors.initials: KY
authors.lastname: Siu
authors.forename: Wing Yan
authors.initials: WY
authors.lastname: Ng
authors.forename: Hsiao Chang
authors.initials: HC
authors.lastname: Chan
authors.forename: Julian A
authors.initials: JA
authors.lastname: Tanner
authors.forename: Sidney
authors.initials: S
authors.lastname: Yu
mesh_terms: D000818:Animals; D022181:COP-Coated Vesicles / Q000378:metabolism*; D019556:COS Cells; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002522:Chlorocebus aethiops; D000072159:Dynactin Complex; D006056:Golgi Apparatus / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D015151:Immunoblotting; D047468:Immunoprecipitation; D036341:Intercellular Signaling Peptides and Proteins; D007425:Intracellular Membranes / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D022101:Microtubule-Organizing Center / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism; D011485:Protein Binding; D034622:RNA Interference; D014162:Transfection; D033921:Vesicular Transport Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; C000606360:DCTN1 protein, human; D000072159:Dynactin Complex; D036341:Intercellular Signaling Peptides and Proteins; D008869:Microtubule-Associated Proteins; C530640:SEC24C protein, human; C503887:TRAPPC9 protein, human; D033921:Vesicular Transport Proteins; D049452:Green Fluorescent Proteins