title: [Spermicidal effect of alcohol extracts from different ratios of Sophora flavescens Ait/Chinese Bulbul in vitro].
abstract: OBJECTIVE
  To observe the spermicidal effect of alcohol extracts from different ratios of Sophora flavescens Ait/Chinese Bulbul in vitro.
  
  
  METHODS
  Semen samples aseptically obtained by masturbation and prepared by density gradient centrifugation from 15 healthy men were incubated in the alcohol extracts from 9 different ratios of Sophora flavescens Ait/Chinese Bulbul for 20 seconds, 2 minutes and 4 minutes. Then the motility and movement parameters of the sperm were detected by computer-assisted semen analysis, and the minimal effective concentrations of the instant spermicidal effect of the extracts were determined.
  
  
  RESULTS
  At the ratio of 3:1, the extract at 0.5 mg/ml significantly inhibited the sperm motility and other sperm movement parameters VCL, VSL, VAP, ALH, WOB and MAD, as compared with the control group. The minimal effective concentration of the instant spermicidal effect of the extracts was 3.5 mg/ml at 3:1.
  
  
  CONCLUSIONS
  The alcohol extracts from Sophora flavescens Ait and Chinese Bulbul at the ratio of 3:1 have the best spermicidal effect in vitro.
authors.affiliation: Department of Urology, Tongji Hospital, Tongji Medical College, Hubei 430030, China.
authors.forename: Xiang-hu
authors.initials: XH
authors.lastname: Meng
authors.forename: Can-feng
authors.initials: CF
authors.lastname: Lu
authors.forename: Guang-hui
authors.initials: GH
authors.lastname: Zang
authors.forename: Long-chang
authors.initials: LC
authors.lastname: Fan
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Ding
authors.forename: Qian
authors.initials: Q
authors.lastname: Su
authors.forename: Chun-ping
authors.initials: CP
authors.lastname: Yin
authors.forename: Ji-hong
authors.initials: JH
authors.lastname: Liu
mesh_terms: D000328:Adult; D006801:Humans; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D031946:Pulsatilla; D055101:Semen Analysis; D029909:Sophora; D013081:Sperm Motility / Q000187:drug effects; D013089:Spermatocidal Agents / Q000494:pharmacology*; D013094:Spermatozoa / Q000187:drug effects*; D055815:Young Adult
chemical_list: D010936:Plant Extracts; D013089:Spermatocidal Agents