title: Genomic characterization of a cell-culture-adapted Korean human G9P[8] rotavirus, CAU05-202.
abstract: The human rotavirus G9 strain is the fifth most common rotavirus worldwide. A human rotavirus G9P[8] strain CAU05-202 was isolated from a young child with diarrhea using a cell culture system, and its major gene sequences were determined. Phylogenetic analysis of the VP7 gene revealed that CAU05-202 clustered into genetic lineage III-d and was most closely related to G9 rotaviruses from Turkey (strain GUH13) and Sri Lanka (strain 05SLC056 and 05SLC057). VP4 and NSP4 gene analysis showed that CAU05-202 belongs to the P[8]-3 lineage and genotype B, respectively. In addition, CAU05-202 has a long RNA electropherotype, supported by VP6 gene analysis, which is clearly associated with subgroup II specificity. Analysis of the G9 rotavirus strain CAU05-202 provides information concerning the genetic relationships among global rotavirus G9 strains, suggesting that closely related G9 strains are persistent and widespread in Asian countries.
authors.affiliation: Department of Internal Medicine, College of Medicine, Chung-Ang University, Seoul, South Korea.
authors.forename: Jong Wook
authors.initials: JW
authors.lastname: Shin
authors.forename: Van Phan
authors.initials: VP
authors.lastname: Le
authors.forename: Van Thai
authors.initials: VT
authors.lastname: Than
authors.forename: Inseok
authors.initials: I
authors.lastname: Lim
authors.forename: Yoosik
authors.initials: Y
authors.lastname: Yoon
authors.forename: Kijeong
authors.initials: K
authors.lastname: Kim
authors.forename: Sang-In
authors.initials: SI
authors.lastname: Chung
authors.forename: Soon Chul
authors.initials: SC
authors.lastname: Myung
authors.forename: Wonyong
authors.initials: W
authors.lastname: Kim
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000235:genetics; D036022:Capsid Proteins / Q000235:genetics; D018929:Cell Culture Techniques; D016000:Cluster Analysis; D003967:Diarrhea / Q000821:virology; D016679:Genome, Viral; D005838:Genotype; D006023:Glycoproteins / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D056910:Republic of Korea; D012401:Rotavirus / Q000235:genetics* / Q000254:growth & development* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000821:virology; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012692:Serial Passage; D014118:Toxins, Biological / Q000235:genetics; D017361:Viral Nonstructural Proteins / Q000235:genetics
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; D006023:Glycoproteins; C081369:NS28 protein, rotavirus; D012367:RNA, Viral; D014118:Toxins, Biological; C060339:VP4 protein, Rotavirus; C061332:VP6 protein, Rotavirus; C050779:VP7 protein, Rotavirus; D017361:Viral Nonstructural Proteins