title: A high density SNP genotyping approach within the 19q13 chromosome region identifies an association of a CNOT3 polymorphism with ankylosing spondylitis.
abstract: OBJECTIVE
  To identify genomic variants in the 19q13 chromosome region associated with ankylosing spondylitis (AS) in human leucocyte antigen (HLA)-B27-positive populations.
  
  
  METHODS
  High-throughput genotyping of 1536 haplotype-tag single nucleotide polymorphisms (SNPs) was performed in 249 patients with AS and 302 healthy controls. Some of the identified associations were validated by genotyping four SNPs in two additional cohorts consisting of 412 cases/301 controls and 144 cases/203 controls. All individuals selected (both cases and controls) were HLA-B27-positive.
  
  
  RESULTS
  Two markers in two different genes (CNOT3 and LAIR2) showed significant association (p<10(-3)) with AS. In addition, sliding windows analysis showed association of groups of adjacent SNPs in regions located around CNOT3 (Chr19: 59347459-59356564, p=2.43 × 10(-4) to 6.54 × 10(-4)). The associations were validated by genotyping four SNPs from regions located near LAIR2 and CNOT3 genes (rs1055234, rs8111398, rs2287828 and rs4591276) in two additional cohorts. The CNOT3 polymorphism (rs1055234) remained associated with AS (combined p=9.73 × 10(-6)). One SNP, located downstream of KIR3DL1, was detected which, tested in combination with HLA-Bw4I80, was associated with AS.
  
  
  CONCLUSIONS
  A novel significant association was detected between SNP rs1055234 and AS susceptibility.
authors.affiliation: Department of Immunology, Hospital Universitario Central de Asturias, Oviedo, Spain.
authors.forename: Roberto
authors.initials: R
authors.lastname: Díaz-Peña
authors.forename: Ana M
authors.initials: AM
authors.lastname: Aransay
authors.forename: Beatriz
authors.initials: B
authors.lastname: Suárez-Álvarez
authors.forename: Jacome
authors.initials: J
authors.lastname: Bruges-Armas
authors.forename: Naiara
authors.initials: N
authors.lastname: Rodríguez-Ezpeleta
authors.forename: María
authors.initials: M
authors.lastname: Regueiro
authors.forename: Fernando M
authors.initials: FM
authors.lastname: Pimentel-Santos
authors.forename: Juan
authors.initials: J
authors.lastname: Mulero
authors.forename: Alejandra
authors.initials: A
authors.lastname: Sánchez
authors.forename: Eduardo
authors.initials: E
authors.lastname: Collantes
authors.forename: Rubén
authors.initials: R
authors.lastname: Queiro
authors.forename: Javier
authors.initials: J
authors.lastname: Ballina
authors.forename: Helena
authors.initials: H
authors.lastname: Alves
authors.forename: Carlos
authors.initials: C
authors.lastname: López-Larrea
mesh_terms: D002888:Chromosomes, Human, Pair 19; D015331:Cohort Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D015796:HLA-B27 Antigen / Q000032:analysis / Q000235:genetics; D057166:High-Throughput Screening Assays; D006801:Humans; D020641:Polymorphism, Single Nucleotide; D011971:Receptors, Immunologic; D013167:Spondylitis, Ankylosing / Q000175:diagnosis / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
chemical_list: C466622:CNOT3 protein, human; D015796:HLA-B27 Antigen; C431503:LAIR-2 receptor; D011971:Receptors, Immunologic; D014157:Transcription Factors