title: Reduced fluoresceinamine as a fluorescent sensor for nitric oxide.
abstract: A new fluorescent sensor for nitric oxide (NO) is presented that is based on its reaction with a non fluorescent substance, reduced fluoresceinamine, producing the highly fluorescent fluoresceinamine. Using a portable homemade stabilized light source consisting of 450 nm LED and fiber optics to guide the light, the sensor responds linearly within seconds in the NO concentration range between about 10-750 μM with a limit of detection (LOD) of about 1 μM. The system generated precise intensity readings, with a relative standard deviation of less than 1%. The suitability of the sensor was assessed by monitoring the NO generated by either the nitrous acid decomposition reaction or from a NO-releasing compound. Using relatively high incubation times, the sensor also responds quantitatively to hydrogen peroxide and potassium superoxide, however, using transient signal measurements results in no interfering species.
authors.affiliation: REQUIMTE, Instituto Superior de Engenharia do Porto, R. António Bernardino Almeida 431, 4200-072 Porto, Portugal. ajd@isep.ipp.pt
authors.forename: Abel J
authors.initials: AJ
authors.lastname: Duarte
authors.forename: Joaquim C G Esteves
authors.initials: JC
authors.lastname: da Silva
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D005336:Fiber Optic Technology / Q000295:instrumentation; D005452:Fluoresceins / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D006834:Hydrazines / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry; D057230:Limit of Detection; D016014:Linear Models; D009569:Nitric Oxide / Q000032:analysis*; D015203:Reproducibility of Results; D013481:Superoxides / Q000737:chemistry
chemical_list: D005452:Fluoresceins; D005456:Fluorescent Dyes; D006834:Hydrazines; D013481:Superoxides; C039316:potassium superoxide; D009569:Nitric Oxide; C084012:1,1-diethyl-2-hydroxy-2-nitrosohydrazine; D006861:Hydrogen Peroxide