title: Validation of a mass spectrometry method to quantify oak ellagitannins in wine samples.
abstract: Detection and individual quantification of oak wood ellagitannins in oak barrel aged red wine samples are difficult mainly due to their low levels and the similarity between their structures. In this work, a quantification method using mass spectrometry has been developed and validated to quantify wine ellagitannins after sample fractionation with a previously reported method. The use of an internal standard is a requirement to correct mass signal variability. (-)-Gallocatechin, among the different tested compounds, was the only one that proved to be a suitable internal standard making possible the accurate and individual quantification of the main oak wood ellagitannins. The developed methodology has been used to detect and quantify these ellagitannins in different Spanish commercial wines, proving its usefulness.
authors.affiliation: Grupo de Investigación en Polifenoles (GIP), Unidad de Nutrición y Bromatología, Facultad de Farmacia, Universidad de Salamanca, Campus Miguel de Unamuno, 37007 Salamanca, Spain.
authors.forename: Ignacio
authors.initials: I
authors.lastname: García-Estévez
authors.forename: M Teresa
authors.initials: MT
authors.lastname: Escribano-Bailón
authors.forename: Julián C
authors.initials: JC
authors.lastname: Rivas-Gonzalo
authors.forename: Cristina
authors.initials: C
authors.lastname: Alcalde-Eon
mesh_terms: D002138:Calibration; D047348:Hydrolyzable Tannins / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D029963:Quercus / Q000737:chemistry*; D013030:Spain; D014920:Wine / Q000032:analysis*; D014934:Wood / Q000737:chemistry
chemical_list: D047348:Hydrolyzable Tannins