title: Gut region-specific diabetic damage to the capillary endothelium adjacent to the myenteric plexus.
abstract: OBJECTIVE
  Damage in the capillaries supplying the MP has been proposed as a critical factor in the development of diabetic enteric neuropathy. We therefore investigated connections between STZ-induced diabetes and the BM morphology, the size of caveolar compartments, the width of TJs, the transport of albumin, and the quantitative features of Cav-1 and eNOS expression in these microvessels.
  
  
  METHODS
  Gut segments from diabetic rats were compared with those from insulin-treated diabetics and those from controls. The effects of diabetes on the BM, the caveolar compartments, and the TJs were evaluated morphometrically. The quantitative features of the albumin transport were investigated by postembedding immunohistochemistry. The diabetes-related changes in Cav-1 and eNOS expression were assessed by postembedding immunohistochemistry and molecular method.
  
  
  RESULTS
  Thickening of the BM, enlargement of the caveolar compartments, opening of the junctions, enhanced transport of albumin, and overexpression of Cav-1 and eNOS were documented in diabetic animals. Insulin replacement in certain gut segments prevented the development of these alterations.
  
  
  CONCLUSIONS
  These data provide morphological, functional, and molecular evidence that the endothelial cells in capillaries adjacent to the MP is a target of diabetic damage in a regional manner.
authors.affiliation: Department of Physiology, Anatomy and Neuroscience, University of Szeged, Szeged, Hungary.
authors.forename: Nikolett
authors.initials: N
authors.lastname: Bódi
authors.forename: Petra
authors.initials: P
authors.lastname: Talapka
authors.forename: Marietta Zita
authors.initials: MZ
authors.lastname: Poles
authors.forename: Edit
authors.initials: E
authors.lastname: Hermesz
authors.forename: Zsanett
authors.initials: Z
authors.lastname: Jancsó
authors.forename: Zója
authors.initials: Z
authors.lastname: Katarova
authors.forename: Ferenc
authors.initials: F
authors.lastname: Izbéki
authors.forename: Tibor
authors.initials: T
authors.lastname: Wittmann
authors.forename: Éva
authors.initials: É
authors.lastname: Fekete
authors.forename: Mária
authors.initials: M
authors.lastname: Bagyánszki
mesh_terms: D000818:Animals; D051242:Caveolin 1 / Q000096:biosynthesis*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism* / Q000473:pathology; D004730:Endothelium, Vascular / Q000293:injuries / Q000378:metabolism* / Q000473:pathology; D005786:Gene Expression Regulation; D008297:Male; D009197:Myenteric Plexus / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology; D052250:Nitric Oxide Synthase Type III / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar
chemical_list: C495537:Cav1 protein, rat; D051242:Caveolin 1; D052250:Nitric Oxide Synthase Type III; C496316:Nos3 protein, rat