title: Comparative characteristic of the inflammatory diterpenes in the roots of Euphorbia fischeriana with different preparation method using HPLC-ELSD.
abstract: A rapid and simple method was established for the simultaneous determination of ten diterpenes by reversed phase HPLC coupled with evaporative light scattering detection. Chromatographic separation was carried out in gradient mode by using a WondaSil™ C(18) column (250mm×4.6mm, 5μm) with mobile phases of methanol and water at 1mL/min. The drift tube temperature of evaporative light scattering detector was set to 65°C and nitrogen flow-rate was 2.7L/min. The method validated was shown to be specific, precise, accurate and linear. Moreover, it was applied to investigate four samples of E. fischeriana with different extracting methods. Contrast to the dried roots, the fresh roots had much higher content of prostratin which represented much higher inflammatory effects than other diterpenes. The results demonstrated that the dried roots were suitable for the ordinary therapy to avoid intense stimulatory, while the fresh roots could be used in the anticancer treatment. All of the results suggested that comparative analysis of chemical components as biomarker and connecting toxic effects of an herb was helpful for revealing the mechanism of its toxicity, and for guiding safer and better application of the herb in TCM clinic.
authors.affiliation: Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing 210046, China. yupingtang@njutcm.edu.cn
authors.forename: Yuping
authors.initials: Y
authors.lastname: Tang
authors.forename: Wei
authors.initials: W
authors.lastname: Jiang
authors.forename: Qicheng
authors.initials: Q
authors.lastname: Wu
authors.forename: Li
authors.initials: L
authors.lastname: Yu
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Weiwei
authors.initials: W
authors.lastname: Tao
authors.forename: Anwei
authors.initials: A
authors.lastname: Ding
authors.forename: Fenqiang
authors.initials: F
authors.lastname: You
authors.forename: Jin-Ao
authors.initials: JA
authors.lastname: Duan
mesh_terms: D000818:Animals; D002273:Carcinogens / Q000032:analysis / Q000633:toxicity; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004224:Diterpenes / Q000032:analysis / Q000627:therapeutic use / Q000633:toxicity*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000627:therapeutic use / Q000633:toxicity*; D028481:Euphorbia / Q000737:chemistry / Q000633:toxicity*; D007249:Inflammation / Q000139:chemically induced*; D008027:Light; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010703:Phorbol Esters / Q000032:analysis / Q000633:toxicity*; D018517:Plant Roots / Q000737:chemistry / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012542:Scattering, Radiation
chemical_list: D002273:Carcinogens; D004224:Diterpenes; D004365:Drugs, Chinese Herbal; D010703:Phorbol Esters; C070999:prostratin