title: Production and radioprotective effects of pyrroloquinoline quinone.
abstract: Pyrroloquinoline quinone (PQQ) was produced by fermentation of the Methylovorus sp. MP688 strain and purified by ion-exchange chromatography, crystallization and recrystallization. The yield of PQQ reached approximately 125 mg/L and highly pure PQQ was obtained. To determine the optimum dose of PQQ for radioprotection, three doses (2 mg/kg, 4 mg/kg, 8 mg/kg) of PQQ were orally administrated to the experimental animals subjected to a lethal dose of 8.0 Gy in survival test. Survival of mice in the irradiation + PQQ (4 mg/kg) group was found to be significantly higher in comparison with the irradiation and irradiation + nilestriol (10 mg/kg) groups. The numbers of hematocytes and bone marrow cells were measured for 21 days after sublethal 4 Gy gamma-ray irradiation with per os of 4 mg/kg of PQQ. The recovery of white blood cells, reticulocytes and bone marrow cells in the irradiation + PQQ group was faster than that in the irradiation group. Furthermore, the recovery of bone marrow cell in the irradiation + PQQ group was superior to that in irradiation + nilestriol group. Our results clearly indicate favourable effects on survival under higher lethal radiation doses and the ability of pyrroloquinoline quinine to enhance haemopoietic recovery after sublethal radiation exposure.
authors.affiliation: Laboratory of Microorganism Engineering, Beijing Institute of Biotechnology, Beijing 100071, China. xiongxianghua@hotmail.com
authors.forename: Xiang-Hua
authors.initials: XH
authors.lastname: Xiong
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Xin
authors.initials: X
authors.lastname: Ge
authors.forename: Shou-Jun
authors.initials: SJ
authors.lastname: Yuan
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Wang
authors.forename: Jing-Juan
authors.initials: JJ
authors.lastname: Zhi
authors.forename: Yan-Xin
authors.initials: YX
authors.lastname: Yang
authors.forename: Bao-Hua
authors.initials: BH
authors.lastname: Du
authors.forename: Wan-Jun
authors.initials: WJ
authors.lastname: Guo
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Wang
authors.forename: De-Xuan
authors.initials: DX
authors.lastname: Yang
authors.forename: Wei-Cai
authors.initials: WC
authors.lastname: Zhang
mesh_terms: D054508:Acute Radiation Syndrome / Q000188:drug therapy; D000284:Administration, Oral; D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000528:radiation effects; D004359:Drug Therapy, Combination; D004964:Estriol / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000494:pharmacology / Q000627:therapeutic use; D005285:Fermentation; D005720:Gamma Rays; D007962:Leukocytes / Q000187:drug effects* / Q000528:radiation effects; D042762:Methylophilaceae / Q000737:chemistry / Q000378:metabolism; D051379:Mice; D045542:PQQ Cofactor / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D011800:Quinestrol / Q000031:analogs & derivatives; D011837:Radiation-Protective Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D011837:Radiation-Protective Agents; D045542:PQQ Cofactor; C010722:nylestriol; D004964:Estriol; D011800:Quinestrol