title: Impact of RGD nanopatterns grafted onto titanium on osteoblastic cell adhesion.
abstract: This work reports on the synthesis of titanium bone implants functionalized with nanoparticles (NPs) containing Arg-Gly-Asp-Cys peptide (RGDC) and shows the adhesion behavior of cells seeded on these materials. RGDC peptides were first conjugated to a norbornenyl-poly(ethylene oxide) macromonomer (Nb-PEO). Then, functional NPs with a size of ∼300 nm and constituted of polynorbornene core surrounded by poly(ethylene oxide) shell were prepared by ring-opening metathesis polymerization in dispersed medium. The grafting density of these NPs on the titanium surface is up to 2 NPs·μm(-2) (80 pmol of RGDC per cm(-2) of NP surface). Cell adhesion was evaluated using preosteoblast cells (MC3T3-E1). Results of cells cultured for 24 h showed that materials grafted with NPs functionalized with RGDC peptides enhance specific cell adhesion and can create filopodia-like structures among NP sites by stressing the cells.
authors.affiliation: CNRS UMR5629, Laboratoire de Chimie des Polymères Organiques, IPB-ENSCBP, Université de Bordeaux 1 , 16 avenue Pey Berland F-33607 Pessac, France.
authors.forename: Minh Ngoc
authors.initials: MN
authors.lastname: Nguyen
authors.forename: Thomas
authors.initials: T
authors.lastname: Lebarbe
authors.forename: Omar F
authors.initials: OF
authors.lastname: Zouani
authors.forename: Loïc
authors.initials: L
authors.lastname: Pichavant
authors.forename: Marie-Christine
authors.initials: MC
authors.lastname: Durrieu
authors.forename: Valérie
authors.initials: V
authors.lastname: Héroguez
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000378:metabolism; D008422:Materials Testing; D051379:Mice; D053758:Nanoparticles; D009842:Oligopeptides / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000737:chemistry; D019736:Prostheses and Implants; D012886:Skull / Q000166:cytology / Q000378:metabolism; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D009842:Oligopeptides; D011092:Polyethylene Glycols; C047981:arginyl-glycyl-aspartic acid; D014025:Titanium