title: Glucocorticoid treatment independently affects expansion and transdifferentiation of porcine neonatal pancreas cell clusters.
abstract: The purpose of this study was to determine the effects of duration and timing of glucocorticoid treatment on the expansion and differentiation of porcine neonatal pancreas cell clusters (NPCCs) into β-cells. After transplantation of NPCCs, the ductal cyst area and β-cell mass in the grafts both showed positive and negative correlations with duration of dexamethasone (Dx) treatment. Pdx-1 and HNF-3β gene expression was significantly downregulated following Dx treatment, whereas PGC-1α expression increased. Pancreatic duct cell apoptosis significantly increased following Dx treatment, whereas proliferation did not change. Altogether, transdifferentiation of porcine NPCCs into β-cells was influenced by the duration of Dx treatment, which might have been due to the suppression of key pancreatic transcription factors. PGC-1α plays an important role in the expansion and transdifferentiation of porcine NPCCs, and the initial 2 weeks following transplantation of porcine NPCCs is a critical period in determining the final β-cell mass in grafts.
authors.affiliation: Department of Endocrinology and Metabolism, The Catholic University of Korea, Seoul, Korea.
authors.forename: Ji-Won
authors.initials: JW
authors.lastname: Kim
authors.forename: Chenglin
authors.initials: C
authors.lastname: Sun
authors.forename: Sung-Yoon
authors.initials: SY
authors.lastname: Jeon
authors.forename: Young-Hye
authors.initials: YH
authors.lastname: You
authors.forename: Ju-Young
authors.initials: JY
authors.lastname: Shin
authors.forename: Seung-Hwan
authors.initials: SH
authors.lastname: Lee
authors.forename: Jae-Hyoung
authors.initials: JH
authors.lastname: Cho
authors.forename: Chung-Gyu
authors.initials: CG
authors.lastname: Park
authors.forename: Kun-Ho
authors.initials: KH
authors.lastname: Yoon
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D054338:Cell Transdifferentiation / Q000187:drug effects*; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology*; D007328:Insulin / Q000032:analysis; D010179:Pancreas / Q000166:cytology* / Q000187:drug effects*; D013329:Structure-Activity Relationship; D013552:Swine
chemical_list: D007328:Insulin; D003907:Dexamethasone