title: Determination of five nitroimidazole residues in artificial porcine muscle tissue samples by capillary electrophoresis.
abstract: A capillary electrophoresis (CE) method with ultraviolet detection has been developed for simultaneous detection and quantification of five nitroimidazoles including benzoylmetronidazole, dimetridazole, metronidazole, ronidazole, and secnidazole in porcine muscles. Nitroimidazoles in samples were extracted by ethyl acetate with subsequent clean-up by a strong cation exchange solid phase extraction column. The clean extracts were subjected to CE separation with optimal experimental conditions: pH 3.0 running buffer containing 25mM sodium phosphate and 0.10mM tetrabutylammonium bromide, 5s hydrodynamic injection at 0.5psi and 28kV separation voltage. The nitroimidazoles could be monitored and detected at 320nm within 18min. The limits of detection were below 1.0μg/kg and limits of quantification were lower than 3.2μg/kg for all nitroimidazoles in the muscle samples. The recoveries and relative standard deviations were 85.4-96.0, 83.5-92.5, 1.3-3.9, and 1.1-4.2%, respectively for the intra-day and inter-day analyses. The proposed CE method has been successfully applied to determine nitroimidazoles in artificial porcine muscle samples with good accuracy and recovery, demonstrating that it has potential for detection and quantification of multi-nitroimidazole residue in real muscle samples.
authors.affiliation: Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, Nanchong 637000, PR China.
authors.forename: Yingyun
authors.initials: Y
authors.lastname: Lin
authors.forename: Yan
authors.initials: Y
authors.lastname: Su
authors.forename: Xiulin
authors.initials: X
authors.lastname: Liao
authors.forename: Na
authors.initials: N
authors.lastname: Yang
authors.forename: Xiupei
authors.initials: X
authors.lastname: Yang
authors.forename: Martin M F
authors.initials: MM
authors.lastname: Choi
mesh_terms: D000085:Acetates / Q000737:chemistry; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004350:Drug Residues / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry; D006863:Hydrogen-Ion Concentration; D007118:Immunoassay; D057230:Limit of Detection; D009132:Muscles / Q000737:chemistry*; D009593:Nitroimidazoles / Q000032:analysis*; D052616:Solid Phase Extraction; D013552:Swine
chemical_list: D000085:Acetates; D009593:Nitroimidazoles; C007650:ethyl acetate