title: Allergen arrays for antibody screening and immune cell activation profiling generated by parallel lipid dip-pen nanolithography.
abstract: Multiple-allergen testing for high throughput and high sensitivity requires the development of miniaturized immunoassays that allow for a large test area and require only a small volume of the test analyte, which is often available only in limited amounts. Developing such miniaturized biochips containing arrays of test allergens needs application of a technique able to deposit molecules at high resolution and speed while preserving its functionality. Lipid dip-pen nanolithography (L-DPN) is an ideal technique to create such biologically active surfaces, and it has already been successfully applied for the direct, nanoscale deposition of functional proteins, as well as for the fabrication of biochemical templates for selective adsorption. The work presented here shows the application of L-DPN for the generation of arrays of the ligand 2,4-dinitrophenyl[1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-[6-[(2,4-dinitrophenyl)amino]hexanoyl] (DNP)] onto glass surfaces as a model system for detection of allergen-specific Immunoglobin E (IgE) antibodies and for mast cell activation profiling.
authors.affiliation: Karlsruher Institut für Technologie (KIT), Institut für Nanotechnologie (INT), Karlsruhe Nano Micro Facility (KNMF), 76021 Karlsruhe Germany. Sylwia.Sekula-Neuner@kit.edu
authors.forename: Sylwia
authors.initials: S
authors.lastname: Sekula-Neuner
authors.forename: Jana
authors.initials: J
authors.lastname: Maier
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Oppong
authors.forename: Andrew C B
authors.initials: AC
authors.lastname: Cato
authors.forename: Michael
authors.initials: M
authors.lastname: Hirtz
authors.forename: Harald
authors.initials: H
authors.lastname: Fuchs
mesh_terms: D000485:Allergens / Q000276:immunology*; D000818:Animals; D000906:Antibodies / Q000032:analysis* / Q000276:immunology*; D007073:Immunoglobulin E / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*; D008407:Mast Cells / Q000276:immunology; D036103:Nanotechnology / Q000379:methods*; D010713:Phosphatidylcholines / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry*; D051381:Rats; D017452:Receptors, IgG / Q000276:immunology; D013696:Temperature
chemical_list: D000485:Allergens; D000906:Antibodies; D010713:Phosphatidylcholines; D010743:Phospholipids; D017452:Receptors, IgG; D007073:Immunoglobulin E; C017251:1,2-oleoylphosphatidylcholine