title: Chromosomal aberrations in cultured human lymphocytes treated with the fungicide, Thiram.
abstract: In vitro effects of different concentrations of Thiram were tested on human lymphocytes to determine, by means of the chromosome aberrations (CAs) assay, whether this fungicide could induce clastogenic damage. Evidences of the effect of Thiram on human lymphocytes were limited to sister chromatid exchange, micronuclei formation, and comet assays. We evaluated 0.01, 0.1, 1.2, and 12.0 μg/mL of Thiram, where 0.01 μg/mL represent the acceptable daily intake dose set by the World Health Organization and the Food and Agriculture Organization for fruit and vegetables, whereas 0.1, 1.2, and 12.0 μg/mL are its multiple values. Results indicated that human lymphocytes treated in vitro with Thiram at concentrations of 1.20 and 12.0 μg/mL significantly increased CAs frequency, compared with the negative control, whereas at lower concentrations (0.01 and 0.1 μg/mL), this effect was not observed. However, Thiram showed a clastogenic effect also at the concentration value of 1.2 μg/mL that represents a lower value with respect to the residue limits found in Italy for grapes, strawberries, potatoes, tobacco, and other fruits and vegetables. Finally, according to some evidence obtained from the study of other fungicides, Thiram produced a significant reduction in the mitotic index with increasing concentration.
authors.affiliation: Dipartimento di Biologia Animale e dell'Uomo, University of Turin, Torino, Italy. alfredo.santovito@unito.it
authors.forename: Alfredo
authors.initials: A
authors.lastname: Santovito
authors.forename: Piero
authors.initials: P
authors.lastname: Cervella
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Delpero
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005659:Fungicides, Industrial / Q000633:toxicity*; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D008940:Mitotic Index; D015394:Molecular Structure; D009152:Mutagenicity Tests; D018709:Statistics, Nonparametric; D013893:Thiram / Q000737:chemistry / Q000633:toxicity*
chemical_list: D005659:Fungicides, Industrial; D013893:Thiram