title: Comparative platelet proteome analysis reveals an increase of monoamine oxidase-B protein expression in Alzheimer's disease but not in non-demented Parkinson's disease patients.
abstract: Monoamine oxidase-B (Mao-B) catalysing the breakdown of the neurotransmitter dopamine, is known to be involved in the pathophysiology of Parkinson's (PD) and Alzheimer's disease (AD). Increased brain Mao-B activity is associated with AD. This alteration can also be seen in platelets, albeit the cause has hitherto remained elusive. To gain a deeper understanding of the etiology of AD, the platelet proteome was characterised, (2D DIGE pH6-9, including Mao-B) from 150 individuals: 34 AD, 13 vascular dementia, 15 non-demented PD patients, 49 matched controls, 18 oldest old and 21 young individuals. One significant change was noted after applying false discovery rate with the upregulation of the Mao-B expression (30% adjusted P value<0.001; effect size 1.31) in AD compared to age- and sex-matched controls. In contrast, Mao-B levels were unchanged in PD to matched controls. Western blot and mRNA analyses verified these findings. Moreover, Mao-B concentration correlated with age in the cognitive healthy individuals (r=0.53; P<0.001) and PD patients but not in those suffering from AD (r=-0.03; P=0.874). Mao-B activity correlated with the increased Mao-B protein expression in AD (r=0.81; P=0.016). We suggest that Mao-B platelet protein level may serve as a biomarker for age-related dementia, especially AD.
authors.affiliation: Surgical Research Laboratories, Medical University of Vienna, Austria. maria.zellner@meduniwien.ac.at
authors.forename: Maria
authors.initials: M
authors.lastname: Zellner
authors.forename: Michael
authors.initials: M
authors.lastname: Baureder
authors.forename: Eduard
authors.initials: E
authors.lastname: Rappold
authors.forename: Peter
authors.initials: P
authors.lastname: Bugert
authors.forename: Nicole
authors.initials: N
authors.lastname: Kotzailias
authors.forename: Rita
authors.initials: R
authors.lastname: Babeluk
authors.forename: Roland
authors.initials: R
authors.lastname: Baumgartner
authors.forename: Johannes
authors.initials: J
authors.lastname: Attems
authors.forename: Christopher
authors.initials: C
authors.lastname: Gerner
authors.forename: Kurt
authors.initials: K
authors.lastname: Jellinger
authors.forename: Erich
authors.initials: E
authors.lastname: Roth
authors.forename: Rudolf
authors.initials: R
authors.lastname: Oehler
authors.forename: Ellen
authors.initials: E
authors.lastname: Umlauf
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D001792:Blood Platelets / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D008297:Male; D008875:Middle Aged; D008995:Monoamine Oxidase / Q000096:biosynthesis*; D010300:Parkinson Disease / Q000378:metabolism*; D020543:Proteome / Q000096:biosynthesis*; D015854:Up-Regulation
chemical_list: D015415:Biomarkers; D020543:Proteome; D008995:Monoamine Oxidase; D004298:Dopamine