title: Natural vanadium-containing Jeju groundwater inhibits immunoglobulin E-mediated anaphylactic reaction and suppresses eicosanoid generation and degranulation in bone marrow derived-mast cells.
abstract: The high-affinity receptor for immunoglobulin E (IgE) (FcεRI)-mediated activation of mast cells plays an important role in various allergic diseases. To assess the anti-allergic activity of natural vanadium-containing Jeju groundwater (JW), an in vivo passive cutaneous anaphylaxis (PCA) animal model and in vitro mouse bone marrow-derived mast cells (BMMCs) was used. JW inhibited cyclooxygenase-2 (COX-2)-dependent prostaglandin D(2) (PGD(2)) generation in a dose-dependent manner, with a concomitant reduction of COX-2 protein expression in IgE-induced BMMCs. In addition, JW inhibited 5-lipoxygenase (5-LOX)-dependent generation of leukotriene C(4) (LTC(4)) as well as degranulation in a dose-dependent manner. These results demonstrate that JW has dual COX-2/5-LOX inhibitory activity. In addition, vanadium pentoxide (V(2)O(5)), which is the major vanadium component of JW, also inhibited PGD(2) and LTC(4) generation as well as degranulation in IgE-induced BMMCs. Furthermore, oral administration of JW dose-dependently inhibited mast cell-dependent passive anaphylactic reaction in IgE-sensitized mice. Taken together, these results suggest that JW may be useful in regulating mast cell-mediated allergic response through the suppression of eicosanoid generation and degranulation in mast cells.
authors.affiliation: College of Pharmacy, Yeungnam University, Gyeongsan, Republic of Korea.
authors.forename: Xian
authors.initials: X
authors.lastname: Li
authors.forename: Yue
authors.initials: Y
authors.lastname: Lu
authors.forename: Ju Hye
authors.initials: JH
authors.lastname: Yang
authors.forename: Ye
authors.initials: Y
authors.lastname: Jin
authors.forename: Seung-Lark
authors.initials: SL
authors.lastname: Hwang
authors.forename: Hyeun Wook
authors.initials: HW
authors.lastname: Chang
mesh_terms: D000707:Anaphylaxis / Q000188:drug therapy / Q000276:immunology; D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000166:cytology; D002118:Calcium / Q000276:immunology; D015550:Cell Degranulation / Q000187:drug effects; D060587:Groundwater; D007073:Immunoglobulin E / Q000276:immunology; D017997:Leukotriene C4 / Q000276:immunology; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D015230:Prostaglandin D2 / Q000276:immunology; D014639:Vanadium / Q000494:pharmacology*; D014874:Water Pollutants, Chemical / Q000494:pharmacology*
chemical_list: D018926:Anti-Allergic Agents; D014874:Water Pollutants, Chemical; D014639:Vanadium; D017997:Leukotriene C4; D007073:Immunoglobulin E; D015230:Prostaglandin D2; D002118:Calcium