title: [Relationship between pulmonary surfactant-associated protein B polymorphisms and the susceptibility to neonatal respiratory distress syndrome].
abstract: OBJECTIVE
  To study the relationship between pulmonary surfactant-associated protein B (SP-B) gene polymorphisms and their susceptibility to neonatal respiratory distress syndrome (RDS).
  
  
  METHODS
  Eighty-eight preterm infants with RDS (RDS group) and 103 infants without RDS (control group) were enrolled. The genomic DNA was isolated using DNA kits. Polymerase chain reaction with restriction fragment length polymorphism technique was used to detect the genotype and allele frequency of the SP-B -18A/C and SP-B 1580C/T single nucleotide polymorphisms. The association between the polymorphisms and RDS was analyzed.
  
  
  RESULTS
  SP-B -18A/C and SP-B 1580C/T were found to be polymorphic in both RDS and control groups. The frequencies of CC genotype (X2=12.26, P<0.01) and C allele (X2=11.97, P<0.01) of SP-B 1580C/T were significantly higher in the RDS group than in the control group. The C allele significantly increased the risk of RDS (OR=2.26, 95%CI: 1.42-3.60). The frequencies of genotype and allele of SP-B -18A/C showed no significant difference between the two groups.
  
  
  CONCLUSIONS
  SP-B 1580C/T polymorphism contributes to the etiology of RDS and may serve as the susceptibility gene for RDS. The C allele increases the risk of RDS. SP-B -18A/C shows no association with the etiology of RDS.
authors.affiliation: Department of Neonatology, Hainan Provincial People's Hospital, Haikou 570311, China.
authors.forename: Wei-Cheng
authors.initials: WC
authors.lastname: Lu
authors.forename: Wei
authors.initials: W
authors.lastname: Xiang
authors.forename: Ming
authors.initials: M
authors.lastname: Wu
authors.forename: Xu
authors.initials: X
authors.lastname: Zheng
authors.forename: Jing
authors.initials: J
authors.lastname: Lin
authors.forename: Xing-Yue
authors.initials: XY
authors.lastname: Chen
authors.forename: Hai-Bo
authors.initials: HB
authors.lastname: Wei
authors.forename: Duan
authors.initials: D
authors.lastname: Zhan
authors.forename: Chun-Lei
authors.initials: CL
authors.lastname: Li
mesh_terms: D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007231:Infant, Newborn; D020641:Polymorphism, Single Nucleotide; D037701:Pulmonary Surfactant-Associated Protein B / Q000235:genetics*; D012127:Respiratory Distress Syndrome, Newborn / Q000209:etiology / Q000235:genetics*
chemical_list: D037701:Pulmonary Surfactant-Associated Protein B