title: miR-10b promotes cell invasion through RhoC-AKT signaling pathway by             targeting HOXD10 in gastric cancer.
abstract: MicroRNAs play critical roles in tumorigenesis as either oncogenes or tumor             suppressors. As a microRNA induced by Twist, miR-10b function as a metastasis             driver in different types of cancer, in which the downstream target gene HOXD10             is the main mediator. In gastric tumor species, miR-10b levels were dramatically             elevated in lymphoma node metastasis-positive tumor tissues compared with lymphoma             node metastasis-free tumor tissues, and were correlated to dowregulation of HOXD10             expression. In gastric cell lines with distinct degrees of differentiation, miR-10b             was highly expressed in the cell line with strong metastatic ability. In MNK45             cells, inhibition of miR-10b led to abrogation of cell invasion. While in GES-1             cells, miR-10 overexpression resulted in enhancement of invasiveness through translational             inhibition of HOXD10, and constitutive expression of HOXD10 reversed the effects             of miR-10b on cell invasion. Furthermore, either knockdown of RhoC or inhibition             of AKT activation interfered miR-10-induced invasiveness in GES-1 cells. In summary,             these observations suggest that miR-10b can stimulate the upregulation of RhoC             and AKT phosphorylation through targeting HOXD10, thus promoting cell invasion             in gastric tumors.
authors.affiliation: Department of General Surgery, The Second Hospital of Jilin University, Changchun 130041, PR China.
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Liu
authors.forename: Jiaming
authors.initials: J
authors.lastname: Zhu
authors.forename: Hong
authors.initials: H
authors.lastname: Cao
authors.forename: Hui
authors.initials: H
authors.lastname: Ren
authors.forename: Xuedong
authors.initials: X
authors.lastname: Fang
mesh_terms: D002454:Cell Differentiation; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D004789:Enzyme Activation; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D057809:HEK293 Cells; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008207:Lymphatic Metastasis; D008562:Membrane Glycoproteins / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D010766:Phosphorylation; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D051057:Proto-Oncogene Proteins c-akt / Q000037:antagonists & inhibitors / Q000378:metabolism*; D034622:RNA Interference; D011971:Receptors, Immunologic / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D013274:Stomach Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D015854:Up-Regulation; D020741:rho GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D000075024:rhoC GTP-Binding Protein
chemical_list: D018398:Homeodomain Proteins; C491341:LILRB2 protein, human; D008562:Membrane Glycoproteins; D047428:Protein Kinase Inhibitors; D011971:Receptors, Immunologic; D014157:Transcription Factors; C072064:HOXD10 protein, human; D051057:Proto-Oncogene Proteins c-akt; C066690:RHOC protein, human; D020741:rho GTP-Binding Proteins; D000075024:rhoC GTP-Binding Protein