title: Cyclin-dependent kinase 4 is a novel target in micoRNA-195-mediated cell cycle arrest in bladder cancer cells.
abstract: miRNAs are a class of small-noncoding RNAs capable of negatively regulating gene expression. Here, we found that miR-195 is down-regulated in human bladder cancer tissue versus normal adjacent tissue. To better characterize the role of miR-195 in bladder cancer, we conducted gain of function analysis by transfecting bladder cancer cell line T24 with chemically synthesized miR-195 mimic. We identified CDK4, an early G1 cell cycle regulator, as a novel target of miR-195. Selective over-expression of miR-195 could induce G1-phase arrest in T24 cells, and subsequently inhibit T24 cell growth. These findings indicate that miR-195 could be a potential tumor suppressor in bladder cancer.
authors.affiliation: Department of Urology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China.
authors.forename: Yiwei
authors.initials: Y
authors.lastname: Lin
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.forename: Yeqing
authors.initials: Y
authors.lastname: Mao
authors.forename: Yunfu
authors.initials: Y
authors.lastname: Liu
authors.forename: Qiqi
authors.initials: Q
authors.lastname: Mao
authors.forename: Kai
authors.initials: K
authors.lastname: Yang
authors.forename: Xiangyi
authors.initials: X
authors.lastname: Zheng
authors.forename: Liping
authors.initials: L
authors.lastname: Xie
mesh_terms: D001483:Base Sequence; D059447:Cell Cycle Checkpoints / Q000235:genetics* / Q000502:physiology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D051358:Cyclin-Dependent Kinase 4 / Q000235:genetics* / Q000378:metabolism*; D015536:Down-Regulation; D016193:G1 Phase; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D012334:RNA, Neoplasm / Q000235:genetics; D014410:Tumor Stem Cell Assay; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology
chemical_list: C552722:MIRN195 microRNA, human; D035683:MicroRNAs; D012334:RNA, Neoplasm; C495900:CDK4 protein, human; D051358:Cyclin-Dependent Kinase 4