title: Radioligand binding analysis as a tool for quality control of GPCR production for structural characterization: adenosine A(2a)R as a template for study.
abstract: Functional characterization of G protein-coupled receptors is essential to ascertain the suitability of a protein target for downstream studies and to help develop optimal expression and isolation procedures. Radioligand binding analysis is a well-established technique, which allows direct measurement of the amount of functional receptor in a sample. It can be readily applied to both membrane-bound and soluble receptor samples and is an ideal method for monitoring the amount of functional protein at each stage in the expression and isolation process. This unit presents protocols for the radioligand binding analysis of the human adenosine A(2a) receptor and provides examples of how these assays can be used at several stages to help optimize expression, solubilization, and isolation procedures.
authors.affiliation: Membrane Protein Crystallography Group, Division of Molecular Biosciences, Imperial College London, London, United Kingdom.
authors.forename: Shweta
authors.initials: S
authors.lastname: Singh
authors.affiliation: Membrane Protein Crystallography Group, Division of Molecular Biosciences, Imperial College London, London, United Kingdom.
authors.forename: Minghao
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Membrane Protein Crystallography Group, Division of Molecular Biosciences, Imperial College London, London, United Kingdom.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Bertheleme
authors.affiliation: School of Pharmacy, University of Reading, Whiteknights, Reading, United Kingdom.
authors.forename: Elodie
authors.initials: E
authors.lastname: Kara
authors.affiliation: School of Pharmacy, University of Reading, Whiteknights, Reading, United Kingdom.
authors.forename: Philip G
authors.initials: PG
authors.lastname: Strange
authors.affiliation: Membrane Protein Crystallography Group, Division of Molecular Biosciences, Imperial College London, London, United Kingdom.
authors.forename: Bernadette
authors.initials: B
authors.lastname: Byrne
mesh_terms: D002462:Cell Membrane / Q000737:chemistry; D003902:Detergents / Q000737:chemistry; D006801:Humans; D010843:Pichia / Q000737:chemistry; D055550:Protein Stability; D011786:Quality Control; D011869:Radioligand Assay / Q000379:methods* / Q000592:standards; D043705:Receptor, Adenosine A2A / Q000032:analysis / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D015203:Reproducibility of Results; D012995:Solubility; D013329:Structure-Activity Relationship
chemical_list: D003902:Detergents; D043705:Receptor, Adenosine A2A; D011994:Recombinant Proteins