title: Differentiation of PC12 cells in three-dimensional collagen sponges with micropatterned nerve growth factor.
abstract: Micropatterning of biological cues is important for the guided formation of neuronal outgrowth and neuronal differentiation. Nerve growth factor (NGF) was micropatterned in a three-dimensional collagen sponges by using micropatterned ice lines that were composed of collagen and NGF. The micropatterned ice lines were prepared by a dispersing machine. PC12 cells were cultured in the NGF-micropatterned collagen sponges and showed micropatterned neurite outgrowth. The neurite outgrowth followed the micropattern of NGF with more neurite outgrowth in the collagen/NGF lines than in the regions between the collagen/NGF lines. The micropattern of the NGF and the neurite network of the PC12 cells can be manipulated by controlling the micropattern of the NGF. The three-dimensional porous scaffolds prepared by this method will have a potential application for the regeneration and repair of the nervous system.
authors.affiliation: Tissue Regeneration Materials Unit, International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science, Tsukuba, Ibaraki 305-0044, Japan
authors.forename: Hwan Hee
authors.initials: HH
authors.lastname: Oh
authors.forename: Hongxu
authors.initials: H
authors.lastname: Lu
authors.forename: Naoki
authors.initials: N
authors.lastname: Kawazoe
authors.forename: Guoping
authors.initials: G
authors.lastname: Chen
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D005456:Fluorescent Dyes; D008855:Microscopy, Electron, Scanning; D020932:Nerve Growth Factor / Q000494:pharmacology*; D016716:PC12 Cells; D051381:Rats
chemical_list: D005456:Fluorescent Dyes; D020932:Nerve Growth Factor