title: Revision of the O-polysaccharide structure of Yersinia pseudotuberculosis O:1a; confirmation of the function of WbyM as paratosyltransferase.
abstract: An O-polysaccharide was isolated by mild acid degradation at pH 4.5 of the long-chain lipopolysaccharide of Yersinia pseudotuberculosis PB1 (serotype O:1a) and studied using 2D NMR spectroscopy. It was found to contain two uncommon monosaccharides: paratose (3,6-dideoxy-d-ribo-hexose, Par) in the furanose form and 6-deoxy-d-manno-heptose (d-6dmanHep). The following structure of a branched tetrasaccharide repeat (O-unit) with a disaccharide side chain was established: This structure is at variance with the O-polysaccharide structure of Y. pseudotuberculosis O:1a reported earlier (Komandrova, N. A.; Gorshkova, R. P.; Isakov, V. V.; Ovodov, Y. S. Bioorg. Khim.1984, 10, 232-237). A comparative study by high-resolution ESI MS of the short-chain lipopolysaccharides from strain PB1 and a wbyM mutant thereof confirmed the function of wbyM as the paratosyltransferase gene.
authors.affiliation: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia. annakond@gmail.com
authors.forename: Anna N
authors.initials: AN
authors.lastname: Kondakova
authors.forename: Alejandro M
authors.initials: AM
authors.lastname: Sevillano
authors.forename: Rima Z
authors.initials: RZ
authors.lastname: Shaikhutdinova
authors.forename: Buko
authors.initials: B
authors.lastname: Lindner
authors.forename: Nadezhda A
authors.initials: NA
authors.lastname: Komandrova
authors.forename: Svetlana V
authors.initials: SV
authors.lastname: Dentovskaya
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Shashkov
authors.forename: Andrey P
authors.initials: AP
authors.lastname: Anisimov
authors.forename: Mikael
authors.initials: M
authors.lastname: Skurnik
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Knirel
mesh_terms: D002240:Carbohydrate Sequence; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry* / Q000378:metabolism*; D014166:Transferases / Q000235:genetics / Q000378:metabolism*; D015011:Yersinia pseudotuberculosis / Q000201:enzymology* / Q000235:genetics
chemical_list: D019081:O Antigens; D014166:Transferases