title: Protective effects of bone marrow mononuclear cell therapy on lung and heart in an elastase-induced emphysema model.
abstract: We hypothesized that bone marrow-derived mononuclear cell (BMDMC) therapy protects the lung and consequently the heart in experimental elastase-induced emphysema. Twenty-four female C57BL/6 mice were intratracheally instilled with saline (C group) or porcine pancreatic elastase (E group) once a week during 4 weeks. C and E groups were randomized into subgroups receiving saline (SAL) or male BMDMCs (2 × 10(6), CELL) intravenously 3h after the first saline or elastase instillation. Compared to E-SAL group, E-CELL mice showed, at 5 weeks: lower mean linear intercept, neutrophil infiltration, elastolysis, collagen fiber deposition in alveolar septa and pulmonary vessel wall, lung cell apoptosis, right ventricle wall thickness and area, higher endothelial growth factor and insulin-like growth factor mRNA expressions in lung tissue, and reduced platelet-derived growth factor, transforming growth factor-β, and caspase-3 expressions. In conclusion, BMDMC therapy was effective at modulating the inflammatory and remodeling processes in the present model of elastase-induced emphysema.
authors.affiliation: Laboratory of Pulmonary Investigation, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: Fernanda F
authors.initials: FF
authors.lastname: Cruz
authors.forename: Mariana A
authors.initials: MA
authors.lastname: Antunes
authors.forename: Soraia C
authors.initials: SC
authors.lastname: Abreu
authors.forename: Lívia C
authors.initials: LC
authors.lastname: Fujisaki
authors.forename: Johnatas D
authors.initials: JD
authors.lastname: Silva
authors.forename: Débora G
authors.initials: DG
authors.lastname: Xisto
authors.forename: Tatiana
authors.initials: T
authors.lastname: Maron-Gutierrez
authors.forename: Debora S
authors.initials: DS
authors.lastname: Ornellas
authors.forename: Vanessa K
authors.initials: VK
authors.lastname: Sá
authors.forename: Nazareth N
authors.initials: NN
authors.lastname: Rocha
authors.forename: Vera L
authors.initials: VL
authors.lastname: Capelozzi
authors.forename: Marcelo M
authors.initials: MM
authors.lastname: Morales
authors.forename: Patricia R M
authors.initials: PR
authors.lastname: Rocco
mesh_terms: D056151:Airway Remodeling; D000704:Analysis of Variance; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D053148:Caspase 3 / Q000378:metabolism; D004452:Echocardiography; D004646:Emphysema / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D005260:Female; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000637:transplantation*; D008168:Lung / Q000378:metabolism / Q000473:pathology*; D016131:Lymphocyte Subsets / Q000166:cytology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D010196:Pancreatic Elastase; D011660:Pulmonary Heart Disease / Q000517:prevention & control*; D011897:Random Allocation
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D010196:Pancreatic Elastase; D053148:Caspase 3