title: Osteoblast-like cell response to calcium phosphate coating chemistry and morphology on etched silicon surfaces.
abstract: Being able to control the behaviour of osteoblast-like cells on a surface may provide a genuine insight into the material surface characteristics and help in creating a successful coating/cell interface. The possibility of creating a micro-environment that can induce proliferation, differentiation and mineralisation of bone cells in vitro, by successfully combining both chemistry and topography of a micro-fabricated substrate is an area that requires a multi-disciplinary approach. Utilising sputter deposition, a process that lends itself to high processability, in conjunction with photolithography allowing for the creation of highly repeatable etched surfaces, we aim to provide a successful combination of chemistry and topography. Correlating the substrate conditions with resultant osteoblast biological function and activity can ultimately be used with a view to modulating the behavior of osteoblast-like cells in vitro.
authors.affiliation: Northern Ireland Bioengineering Centre, University of Ulster, Newtownabbey, Northern Ireland, UK. g.burke@ulster.ac.uk
authors.forename: George A
authors.initials: GA
authors.lastname: Burke
authors.forename: Chris J
authors.initials: CJ
authors.lastname: Rea
authors.forename: Fergal G
authors.initials: FG
authors.lastname: Horgan
authors.forename: Marie
authors.initials: M
authors.lastname: Turkington
authors.forename: Adrian R
authors.initials: AR
authors.lastname: Boyd
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Meenan
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D002130:Calcium Phosphates / Q000737:chemistry*; D002460:Cell Line; D006801:Humans; D010006:Osteoblasts / Q000166:cytology*; D012825:Silicon / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D002130:Calcium Phosphates; C020243:calcium phosphate; D000469:Alkaline Phosphatase; D012825:Silicon