title: Angiogenic microRNA-210 is present in cells surrounding osteonecrosis.
abstract: A role of microRNAs (miRNAs), which are ≈ 22-nucleotide non-coding RNAs, has recently been recognized in human diseases. The objective of this study was to identify the expression pattern of miRNA (miR)-210, known to be associated with angiogenesis, in bone from patients with osteonecrosis (ON) of the femoral head. The expression of miR-210 in bone from 10 patients with osteoarthritis (OA) of the hip and ten with ON was analyzed by quantitative reverse transcription-polymerase chain reaction (RT-PCR) and by in situ hybridization. In addition, immunohistochemical staining for von Willebrand factor (vWF) and vascular endothelial growth factor (VEGF) was performed to identify the miR-210 expressing cells. We found that in ON samples, the expression of mature, primary miR-210, VEGF, matrix metalloproteinase (MMP)-2, and MMP-7 was significantly higher than that of OA samples. Section in situ hybridization of mature miR-210 revealed that mature miR-210 is expressed around the necrotic area. vWF and VEGF were also strongly expressed in the miR-210 expressing cells. This study shows that miR-210 is intensely expressed in ON, and might play a role in ON pathogenesis. The present study provides a solid basis for further functional analyses of miRNAs in ON.
authors.affiliation: Department of Orthopaedic Surgery, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan. signori0726@go8.enjoy.ne.jp
authors.forename: Keiichiro
authors.initials: K
authors.lastname: Yamasaki
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Nakasa
authors.forename: Shigeru
authors.initials: S
authors.lastname: Miyaki
authors.forename: Takuma
authors.initials: T
authors.lastname: Yamasaki
authors.forename: Yuji
authors.initials: Y
authors.lastname: Yasunaga
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Ochi
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000096:biosynthesis; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis; D020783:Matrix Metalloproteinase 7 / Q000096:biosynthesis; D035683:MicroRNAs / Q000096:biosynthesis*; D008875:Middle Aged; D015207:Osteoarthritis, Hip / Q000235:genetics* / Q000378:metabolism; D010020:Osteonecrosis / Q000235:genetics* / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis; D014841:von Willebrand Factor / Q000096:biosynthesis
chemical_list: C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; C543800:MIRN210 microRNA, human; D035683:MicroRNAs; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D014841:von Willebrand Factor; C060932:MMP7 protein, human; D020783:Matrix Metalloproteinase 7; D020778:Matrix Metalloproteinase 2