title: Human blood identification using the genome profiling method.
abstract: In criminal investigations, usually it is necessary to identify whether blood spots found at crime scenes are from humans or not. Nowadays, immunohistochemical methods and DNA analysis are usually used for this purpose. However, such methods and DNA analysis are labor intensive and expensive, and require highly trained skilled technicians. Recently, the genome profiling method (GP method) was developed. However, its use as a human DNA analysis method has not been reported. In this report, an attempt was made to differentiate human blood samples from animal blood samples using the GP method for forensic purposes. DNA extracted from a rat, squirrel, cat, dog, cow, and antelope along with human blood samples were analyzed. Following cluster analysis the human samples clustered into a single group separate from the animal samples. Therefore, although the number of samples was small the results suggest that the GP method might enable us to differentiate human samples from various animal samples. It may become a powerful tool in the field of forensic science.
authors.affiliation: Department of Forensic Medicine, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan.
authors.forename: Nagisa
authors.initials: N
authors.lastname: Suwa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ikegaya
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Takasaka
authors.forename: Koichi
authors.initials: K
authors.lastname: Nishigaki
authors.forename: Koichi
authors.initials: K
authors.lastname: Sakurada
mesh_terms: D000818:Animals; D001769:Blood; D016172:DNA Fingerprinting / Q000379:methods*; D005554:Forensic Medicine; D016678:Genome / Q000235:genetics*; D006801:Humans