title: High frequency of Neuropeptide Y Leu7Pro polymorphism in an Iranian population and its association with coronary artery disease.
abstract: OBJECTIVE
  Neuropeptide Y (NPY), a 36-amino acid peptide, is widely expressed in the central and peripheral nervous systems as well as in the heart. A relationship has been reported between NPY gene variants and coronary artery disease (CAD) in some populations. However, there are few data on the NPY gene polymorphism and CAD in the Persian population. In the current study we have investigated the relationship between the NPY Leu7Pro polymorphism and CAD within a population from Iran.
  
  
  METHODS
  A total of 1061 subjects were recruited; 609 patients and 452 healthy subjects. Four hundred and twenty eight of the patients had >50% stenosis; with the remaining 181 patients having <50% stenosis based on angiography. Angiography positive patients were divided into three groups: those with single (n=115), double (n=140), and triple vessel (n=173) disease. DNA analysis for the Leu7Pro polymorphism was performed using a PCR-RFLP technique.
  
  
  RESULTS
  A significantly higher frequency of the Leu7Pro genotype was observed in CAD patients compared with the control group (P<0.05). Patients with the Pro7 had significantly higher values for weight (P=0.027), BMI (P=0.001), hip circumference (P=0.003) and prevalence of diabetes mellitus (P=0.018) but reduced prevalence of a history of myocardial infarction (P=0.017).
  
  
  CONCLUSIONS
  The frequency of Leu7Pro polymorphism of NPY was 5.9% in our Iranian population; higher than reported for other Asian populations. The Leu7Pro polymorphism was associated with CAD in an Iranian population.
authors.affiliation: Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran. Alimk31@yahoo.com
authors.forename: Ali
authors.initials: A
authors.lastname: Masoudi-Kazemabad
authors.forename: Khadijeh
authors.initials: K
authors.lastname: Jamialahmadi
authors.forename: Mohsen
authors.initials: M
authors.lastname: Moohebati
authors.forename: Majid
authors.initials: M
authors.lastname: Mojarrad
authors.forename: Raheleh
authors.initials: R
authors.lastname: Dehghan-Manshadi
authors.forename: Mohammad Mahdi
authors.initials: MM
authors.lastname: Forghanifard
authors.forename: Saeed
authors.initials: S
authors.lastname: Akhlaghi
authors.forename: Gordon A
authors.initials: GA
authors.lastname: Ferns
authors.forename: Majid
authors.initials: M
authors.lastname: Ghayour-Mobarhan
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019943:Amino Acid Substitution / Q000235:genetics / Q000502:physiology; D016022:Case-Control Studies; D003324:Coronary Artery Disease / Q000150:complications / Q000453:epidemiology / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005828:Genetics, Population; D006801:Humans; D007492:Iran / Q000453:epidemiology; D007930:Leucine / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009478:Neuropeptide Y / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000502:physiology; D015995:Prevalence; D011392:Proline / Q000235:genetics; D055815:Young Adult
chemical_list: D009478:Neuropeptide Y; D011392:Proline; D007930:Leucine