title: Programmable self-assembly of antibody-oligonucleotide conjugates as small molecule and protein carriers.
abstract: Dihydrofolate reductase single-chain variable fragment (scFv) fusion proteins can be used for the targeted cellular delivery of oligonucleotides, conjugated small molecules, and proteins via labeling of oligonucleotides by bis-methotrexate.
authors.affiliation: Department of Medicinal Chemistry, University of Minnesota, Minneapolis, Minnesota 55455, USA.
authors.forename: Amit
authors.initials: A
authors.lastname: Gangar
authors.forename: Adrian
authors.initials: A
authors.lastname: Fegan
authors.forename: Sidath C
authors.initials: SC
authors.lastname: Kumarapperuma
authors.forename: Carston R
authors.initials: CR
authors.lastname: Wagner
mesh_terms: D000906:Antibodies / Q000737:chemistry*; D001896:Boron Compounds / Q000737:chemistry; D017252:CD3 Complex / Q000096:biosynthesis; D002352:Carrier Proteins / Q000737:chemistry*; D045744:Cell Line, Tumor; D002845:Chromatography / Q000379:methods; D019281:Dimerization; D004164:Diphosphonates / Q000737:chemistry; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D008024:Ligands; D008727:Methotrexate / Q000031:analogs & derivatives / Q000737:chemistry; D008956:Models, Chemical; D009841:Oligonucleotides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry; D057127:Single-Chain Antibodies / Q000737:chemistry; D013762:Tetrahydrofolate Dehydrogenase / Q000737:chemistry
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D000906:Antibodies; D001896:Boron Compounds; D017252:CD3 Complex; D002352:Carrier Proteins; D004164:Diphosphonates; D008024:Ligands; D009841:Oligonucleotides; D011506:Proteins; D057127:Single-Chain Antibodies; C097566:methotrexate bisphosphonate; D013762:Tetrahydrofolate Dehydrogenase; D008727:Methotrexate