title: Physiological and proteomic responses of cotton (Gossypium herbaceum L.) to drought stress.
abstract: Cotton genotype RAHS 187 was analyzed for changes in physiology, biochemistry and proteome due to drought stress. The deleterious effect of drought in cotton plants was mainly targeted towards photosynthesis. The gas-exchange parameters of net photosynthesis (A), stomatal conductance (g(s)) and transpiration (E) showed a decreasing trend as the drought intensity increased. The fluorescence parameters of, effective quantum yield of PSII (Φ(PSII)), and electron transport rates (ETR), also showed a declining trend. As the intensity of drought increased, both H(2)O(2) and MDA levels increased indicating oxidative stress. Anthocyanin levels were increased by more than four folds in the droughted plants. Two-dimensional gel electrophoresis detected more than 550 protein spots. Significantly expressed proteins were analyzed by peptide mass ﬁngerprinting (PMF) using MALDI-TOF-TOF. The number of up-regulated spots was found to be 16 while 6 spots were down-regulated. The reasonable implications in drought response of the identified proteins vis-à-vis physiological changes are discussed. Results provide some additional information that can lead to a better understanding of the molecular basis of drought-sensitivity in cotton plants.
authors.affiliation: Plant Physiology Lab, Council of Scientific and Industrial Research - National Botanical Research Institute, Rana Pratap Marg, Lucknow 226 001, U.P., India.
authors.forename: Farah
authors.initials: F
authors.lastname: Deeba
authors.forename: Ashutosh K
authors.initials: AK
authors.lastname: Pandey
authors.forename: Sanjay
authors.initials: S
authors.lastname: Ranjan
authors.forename: Ashwarya
authors.initials: A
authors.lastname: Mishra
authors.forename: Ruchi
authors.initials: R
authors.lastname: Singh
authors.forename: Y K
authors.initials: YK
authors.lastname: Sharma
authors.forename: Pramod A
authors.initials: PA
authors.lastname: Shirke
authors.forename: Vivek
authors.initials: V
authors.lastname: Pandey
mesh_terms: D000222:Adaptation, Physiological / Q000502:physiology*; D000872:Anthocyanins / Q000378:metabolism; D055864:Droughts; D004579:Electron Transport / Q000502:physiology; D005453:Fluorescence; D005838:Genotype; D003368:Gossypium / Q000235:genetics / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology*; D010788:Photosynthesis / Q000502:physiology*; D045332:Photosystem II Protein Complex / Q000502:physiology; D010940:Plant Proteins / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods
chemical_list: D000872:Anthocyanins; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D020543:Proteome; D008315:Malondialdehyde; D006861:Hydrogen Peroxide