title: Influence of an autologous serum-supplemented medium on the proliferation and differentiation into neurons of canine bone marrow stromal cells.
abstract: We investigated the influence of autologous serum (AS)-supplemented medium on the proliferation and differentiation into neurons of canine bone marrow stromal cells (BMSCs). Canine BMSCs were cultured using α-MEM only, α-MEM with 10% fetal bovine serum (FBS), and 5, 10 and 20% AS-supplemented α-MEM. Growth of canine BMSCs was observed in all AS groups. The proliferation capacity of canine BMSCs in the AS groups was similar to that in the FBS group. No significant differences between the FBS and AS groups were observed in the percentage of the cells that changed to the neuron-like morphology and neuron-specific enolase-positive ratio after neuronal differentiation. Canine BMSCs cultured using AS-supplemented medium were able to proliferate and showed neuronal differentiation potency.
authors.affiliation: Laboratory of Veterinary Surgery, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan. edamura@brs.nihon-u.ac.jp
authors.forename: Kazuya
authors.initials: K
authors.lastname: Edamura
authors.forename: Takashi
authors.initials: T
authors.lastname: Kurosawa
authors.forename: Rei
authors.initials: R
authors.lastname: Nakano
authors.forename: Kenji
authors.initials: K
authors.lastname: Teshima
authors.forename: Kazushi
authors.initials: K
authors.lastname: Asano
authors.forename: Shigeo
authors.initials: S
authors.lastname: Tanaka
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology*; D004285:Dogs / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry / Q000662:veterinary; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009474:Neurons / Q000166:cytology*
chemical_list: D003470:Culture Media