title: Influence of cations on growth of thermophilic Geobacillus spp. and Anoxybacillus flavithermus in planktonic culture.
abstract: Free ions of Na(+), K(+), Ca(2+), and Mg(2+) influenced the optical density of planktonic cultures of thermophilic bacilli. Anoxybacillus flavithermus E16 and Geobacillus sp. strain F75 (milk powder manufacturing plant isolates) and A. flavithermus DSM 2641 and G. thermoleovorans DSM 5366 were studied. Ca(2+) and Mg(2+) were associated with increases in optical density more so than Na(+) and K(+). Overall, it appeared that Ca(2+) and/or Mg(2+) was required for the production of protein in thermophilic bacilli, as shown by results obtained with A. flavithermus E16, which was selected for further study.
authors.affiliation: Institute of Food, Nutrition and Human Health, Massey University, Palmerston North, New Zealand. ben.somerton@fonterra.com
authors.forename: Ben
authors.initials: B
authors.lastname: Somerton
authors.forename: Jon
authors.initials: J
authors.lastname: Palmer
authors.forename: John
authors.initials: J
authors.lastname: Brooks
authors.forename: Edward
authors.initials: E
authors.lastname: Smolinski
authors.forename: Denise
authors.initials: D
authors.lastname: Lindsay
authors.forename: Steve
authors.initials: S
authors.lastname: Flint
mesh_terms: D000818:Animals; D058677:Anoxybacillus / Q000187:drug effects / Q000254:growth & development*; D001426:Bacterial Proteins / Q000378:metabolism; D002118:Calcium / Q000494:pharmacology; D002412:Cations / Q000494:pharmacology*; D003470:Culture Media / Q000737:chemistry; D019649:Food Industry; D056506:Geobacillus / Q000187:drug effects / Q000254:growth & development*; D006358:Hot Temperature; D008274:Magnesium / Q000494:pharmacology; D008892:Milk / Q000382:microbiology; D010933:Plankton / Q000187:drug effects / Q000254:growth & development*
chemical_list: D001426:Bacterial Proteins; D002412:Cations; D003470:Culture Media; D008274:Magnesium; D002118:Calcium