title: HPLC-PDA method for quinovic acid glycosides assay in Cat's claw (Uncaria tomentosa) associated with UPLC/Q-TOF-MS analysis.
abstract: Uncaria tomentosa (Willd.) is a medicinal plant largely used in folk medicine due to its wide range of biological activities, many of which are usually ascribed to the two main classes of secondary metabolites, namely, alkaloids and quinovic acid glycosides. In this work, a reversed phase HPLC-PDA method was developed and validated for the assay of quinovic acid glycosides in crude and dried extracts of Uncaria tomentosa (Cat's claw) bark. The validation comprised tests of specificity, accuracy, linearity, intermediate precision, repeatability and limits of detection and of quantification. Alpha-hederin was used as the external standard. High coefficients of determination with lower R.S.D. were achieved for both external standard and crude extract. The structural characterization of the main quinovic acid glycosides presented in the crude extract was carried out through UPLC/Q-TOF-MS. The identities of the compounds were obtained through the comparison of their fragmentation patterns with those reported in the literature. The analytical method was successfully applied for quantifying quinovic acid glycosides in two different dried extracts from U. tomentosa and in one quinovic acid glycosides purified fraction.
authors.affiliation: Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Cabral
authors.initials: C
authors.lastname: Pavei
authors.forename: Samuel
authors.initials: S
authors.lastname: Kaiser
authors.forename: Simone Gasparin
authors.initials: SG
authors.lastname: Verza
authors.forename: Gustavo Luis
authors.initials: GL
authors.lastname: Borre
authors.forename: George Gonzalez
authors.initials: GG
authors.lastname: Ortega
mesh_terms: D020948:Cat's Claw / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006027:Glycosides / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014315:Triterpenes / Q000032:analysis*
chemical_list: D006027:Glycosides; D014315:Triterpenes; C472605:quinovic acid