title: Schnurri regulates hemocyte function to promote tissue recovery after DNA damage.
abstract: Tissue recovery after injury requires coordinated regulation of cell repair and apoptosis, removal of dead cells and regeneration. A critical step in this process is the recruitment of blood cells that mediate local inflammatory and immune responses, promoting tissue recovery. Here we identify a new role for the transcriptional regulator Schnurri (Shn) in the recovery of UV-damaged Drosophila retina. Using an experimental paradigm that allows precise quantification of tissue recovery after a defined dose of UV, we find that Shn activity in the retina is required to limit tissue damage. This function of Shn relies on its transcriptional induction of the PDGF-related growth factor Pvf1, which signals to tissue-associated hemocytes. We show that the Pvf1 receptor PVR acts in hemocytes to induce a macrophage-like morphology and that this is required to limit tissue loss after irradiation. Our results identify a new Shn-regulated paracrine signaling interaction between damaged retinal cells and hemocytes that ensures recovery and homeostasis of the challenged tissue.
authors.affiliation: Department of Biomedical Genetics, University of Rochester Medical Center, Box 633, 601 Elmwood Avenue, Rochester, NY 14642, USA.
authors.forename: Ellen Miriam
authors.initials: EM
authors.lastname: Kelsey
authors.forename: Xi
authors.initials: X
authors.lastname: Luo
authors.forename: Katja
authors.initials: K
authors.lastname: Brückner
authors.forename: Heinrich
authors.initials: H
authors.lastname: Jasper
mesh_terms: D000818:Animals; D004249:DNA Damage / Q000502:physiology* / Q000528:radiation effects; D004268:DNA-Binding Proteins / Q000502:physiology*; D029721:Drosophila Proteins / Q000502:physiology*; D004331:Drosophila melanogaster / Q000502:physiology* / Q000528:radiation effects; D005260:Female; D006434:Hemocytes / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects; D008297:Male; D020127:Recovery of Function / Q000502:physiology / Q000528:radiation effects; D012160:Retina / Q000293:injuries / Q000502:physiology* / Q000528:radiation effects; D014157:Transcription Factors / Q000502:physiology*; D014466:Ultraviolet Rays / Q000009:adverse effects
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D014157:Transcription Factors; C094066:shn protein, Drosophila