title: Proteomic analysis of Trypanosoma cruzi epimastigotes subjected to heat shock.
abstract: Trypanosoma cruzi is exposed to sudden temperature changes during its life cycle. Adaptation to these variations is crucial for parasite survival, reproduction, and transmission. Some of these conditions may change the pattern of genetic expression of proteins involved in homeostasis in the course of stress treatment. In the present study, the proteome of T. cruzi epimastigotes subjected to heat shock and epimastigotes grow normally was compared by two-dimensional gel electrophoresis followed by mass spectrometry for protein identification. Twenty-four spots differing in abundance were identified. Of the twenty-four changed spots, nineteen showed a greater intensity and five a lower intensity relative to the control. Several functional categories of the identified proteins were determined: metabolism, cell defense, hypothetical proteins, protein fate, protein synthesis, cellular transport, and cell cycle. Proteins involved in the interaction with the cellular environment were also identified, and the implications of these changes are discussed.
authors.affiliation: Laboratorio de Estudios Sobre Tripanosomiasis, Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, A.P. 70228, 04510 México, DF, Mexico.
authors.forename: Deyanira
authors.initials: D
authors.lastname: Pérez-Morales
authors.forename: Humberto
authors.initials: H
authors.lastname: Lanz-Mendoza
authors.forename: Gerardo
authors.initials: G
authors.lastname: Hurtado
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Martínez-Espinosa
authors.forename: Bertha
authors.initials: B
authors.lastname: Espinoza
mesh_terms: D000222:Adaptation, Physiological; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D018869:Heat-Shock Response / Q000502:physiology; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D011506:Proteins / Q000032:analysis / Q000145:classification / Q000378:metabolism*; D020543:Proteome / Q000032:analysis / Q000378:metabolism*; D014349:Trypanosoma cruzi / Q000378:metabolism*
chemical_list: D011506:Proteins; D020543:Proteome