title: Association between the perioperative antioxidative ability of platelets and early post-transplant function of kidney allografts: a pilot study.
abstract: BACKGROUND
  Recent studies have demonstrated that the actions of platelets may unfavorably influence post-transplant function of organ allografts. In this study, the association between post-transplant graft function and the perioperative activity of platelet antioxidants was examined among kidney recipients divided into early (EGF), slow (SGF), and delayed graft function (DGF) groups.
  
  
  RESULTS
  Activities of superoxide dismutase, catalase, glutathione transferase (GST), glutathione peroxidase, and glucose-6-phosphate dehydrogenase (G6P) were determined and levels of glutathione, oxidized glutathione, and isoprostane were measured in blood samples collected immediately before and during the first and fifth minutes of renal allograft reperfusion. Our results demonstrated a significant increase in isoprostane levels in all groups. Interestingly, in DGF patients, significantly lower levels of perioperative activity of catalase (p<0.02) and GST (p<0.02) were observed. Moreover, in our study, the activity of platelet antioxidants was associated with intensity of perioperative oxidative stress. For discriminating SGF/DGF from EGF, sensitivity, specificity, and positive and negative predictive values of platelet antioxidants were 81-91%, 50-58%, 32-37%, and 90-90.5%, respectively.
  
  
  CONCLUSIONS
  During renal transplantation, significant changes occur in the activity of platelet antioxidants. These changes seem to be associated with post-transplant graft function and can be potentially used to differentiate between EGF and SGF/DGF. To the best of our knowledge, this is the first study to reveal the potential protective role of platelets in the human transplantation setting.
authors.affiliation: Department of Laboratory Diagnostics and Molecular Medicine, Pomeranian Medical University, Szczecin, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Dołęgowska
authors.forename: Wojciech
authors.initials: W
authors.lastname: Błogowski
authors.forename: Leszek
authors.initials: L
authors.lastname: Domański
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D001792:Blood Platelets / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D051799:Delayed Graft Function; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006085:Graft Survival; D006801:Humans; D016030:Kidney Transplantation / Q000379:methods*; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D018384:Oxidative Stress; D059035:Perioperative Period; D010865:Pilot Projects; D013482:Superoxide Dismutase / Q000378:metabolism; D013997:Time Factors; D014184:Transplantation, Homologous
chemical_list: D000975:Antioxidants; D005954:Glucosephosphate Dehydrogenase; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase