title: Anti-TIF1γ antibodies (anti-p155) in adult patients with dermatomyositis: comparison of different diagnostic assays.
abstract: BACKGROUND
  A new myositis-specific autoantibody (anti-p155) directed against transcriptional intermediary factor 1 γ (TIF1γ) has been described as a good marker of cancer-associated myositis (CAM).
  
  
  OBJECTIVE
  To analyse the feasibility of detecting this autoantibody in patient serum samples using new assays with commercially available recombinant TIF1γ.
  
  
  METHODS
  The study included 90 Spanish patients with dermatomyositis (DM), classified as clinically amyopathic DM, CAM, or DM without cancer. Anti-TIF1γ antibodies were detected by ELISA and immunoblot techniques and compared with anti-p155 antibody detection by protein immunoprecipitation assays with radiolabelled HeLa cells. The κ coefficient was used to compare the agreement between the different tests.
  
  
  RESULTS
  Serum samples from 23 (25.6%) and 20 (22.2%) patients with DM recognised TIF1γ by ELISA and immunoblot, respectively. ELISA (κ=0.91) and immunoblot (κ=0.88) showed excellent agreement with immunoprecipitation analysis (anti-p155). Good concordance (κ=0.91) was also seen between ELISA and immunoblot.
  
  
  CONCLUSIONS
  Excellent agreement was found between anti-p155 detected by immunoprecipitation and anti-TIF1γ detected by ELISA or immunoblot. These data indicate that identification of this autoantibody can be reliably performed in a standard laboratory setting, with potential application in clinical practice for cancer screening in adult patients with DM.
authors.affiliation: Department of Internal Medicine, Vall d’Hebron General Hospital. Universitat Autonoma de Barcelona, Barcelona, Spain. mlabrador@vhebron.net
authors.forename: Moises
authors.initials: M
authors.lastname: Labrador-Horrillo
authors.forename: Maria Angeles
authors.initials: MA
authors.lastname: Martínez
authors.forename: Albert
authors.initials: A
authors.lastname: Selva-O'Callaghan
authors.forename: Ernesto
authors.initials: E
authors.lastname: Trallero-Araguás
authors.forename: Eva
authors.initials: E
authors.lastname: Balada
authors.forename: Miquel
authors.initials: M
authors.lastname: Vilardell-Tarrés
authors.forename: Candido
authors.initials: C
authors.lastname: Juarez
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001323:Autoantibodies / Q000097:blood* / Q000276:immunology; D015415:Biomarkers / Q000097:blood; D003882:Dermatomyositis / Q000175:diagnosis* / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000175:diagnosis / Q000276:immunology; D014157:Transcription Factors / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D015415:Biomarkers; C511956:TRIM33 protein, human; D014157:Transcription Factors