title: Genomic study of Argentinean Equid herpesvirus 1 strains.
abstract: Equid herpesvirus 1 (EHV-1) infection has a significant economic impact on equine production, causing abortion, respiratory disease, neonatal death and neurological disorders. The identification of specific EHV-1 genes related to virulence and pathogenicity has been the aim of several research groups. The purpose of the present study was to analyze different genomic regions of Argentinean EHV-1 strains and to determine their possible relationship with virulence or clinical signs. Twenty-five EHV-1 Argentinean isolates recovered from different clinical cases between 1979 and 2007 and two reference strains were amplified and sequenced. The sequence alignments were carried out using Clustal X version 1.92 and the putative amino acid sequences were deduced using Bio-Edit version 7.05. Minor changes were observed. No changes that could be involved in the different virulence in the mouse model of three EHV-1 Argentinean strains were found. No genetic variants were observed. The genomic regions analyzed are unsuitable for differentiation between abortigenic strains and those isolated from neonatal deaths.
authors.affiliation: Cátedra de Virología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata.
authors.forename: Nadia A
authors.initials: NA
authors.lastname: Fuentealba
authors.forename: Guillermo H
authors.initials: GH
authors.lastname: Sguazza
authors.forename: Matías L
authors.initials: ML
authors.lastname: Eöry
authors.forename: Alejandro R
authors.initials: AR
authors.lastname: Valera
authors.forename: Marcelo R
authors.initials: MR
authors.lastname: Pecoraro
authors.forename: Cecilia M
authors.initials: CM
authors.lastname: Galosi
mesh_terms: D000034:Abortion, Veterinary / Q000453:epidemiology / Q000821:virology; D000595:Amino Acid Sequence; D000818:Animals; D001118:Argentina / Q000453:epidemiology; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D016679:Genome, Viral; D006566:Herpesviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D004861:Herpesvirus 1, Equid / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D006734:Horse Diseases / Q000453:epidemiology / Q000821:virology*; D006736:Horses; D051379:Mice; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014774:Virulence / Q000235:genetics
chemical_list: D004279:DNA, Viral