title: Elucidation mechanism of different biological responses to multi-walled carbon nanotubes using four cell lines.
abstract: We examined differences in cellular responses to multi-walled carbon nanotubes (MWCNTs) using malignant pleural mesothelioma cells (MESO-1), bronchial epithelial cells (BEAS-2B), neuroblastoma cells (IMR-32), and monoblastic cells (THP-1), before and after differentiation. MESO-1, BEAS-2B and differentiated THP-1 cells actively endocytosed MWCNTs, resulting in cytotoxicity with lysosomal injury. However, cytotoxicity did not occur in IMR-32 or undifferentiated THP-1 cells. Both differentiated and undifferentiated THP-1 cells exhibited an inflammatory response. Carbon blacks were endocytosed by the same cell types without lysosomal damage and caused cytokine secretion, but they did not cause cytotoxicity. These results indicate that the cytotoxicity of MWCNTs requires not only cellular uptake but also lysosomal injury. Furthermore, it seems that membrane permeability or cytokine secretion without cytotoxicity results from several active mechanisms. Clarification of the cellular recognition mechanism for MWCNTs is important for developing safer MWCNTs.
authors.affiliation: Institute of Carbon Science and Technology, Shinshu University, Matsumoto, Nagano, Japan. hhaniu@shinshu-u.ac.jp
authors.forename: Hisao
authors.initials: H
authors.lastname: Haniu
authors.forename: Naoto
authors.initials: N
authors.lastname: Saito
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Matsuda
authors.forename: Yoong-Ahm
authors.initials: YA
authors.lastname: Kim
authors.forename: Ki Chul
authors.initials: KC
authors.lastname: Park
authors.forename: Tamotsu
authors.initials: T
authors.lastname: Tsukahara
authors.forename: Yuki
authors.initials: Y
authors.lastname: Usui
authors.forename: Kaoru
authors.initials: K
authors.lastname: Aoki
authors.forename: Masayuki
authors.initials: M
authors.lastname: Shimizu
authors.forename: Nobuhide
authors.initials: N
authors.lastname: Ogihara
authors.forename: Kazuo
authors.initials: K
authors.lastname: Hara
authors.forename: Seiji
authors.initials: S
authors.lastname: Takanashi
authors.forename: Masanori
authors.initials: M
authors.lastname: Okamoto
authors.forename: Norio
authors.initials: N
authors.lastname: Ishigaki
authors.forename: Koichi
authors.initials: K
authors.lastname: Nakamura
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kato
mesh_terms: D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D004705:Endocytosis; D006801:Humans; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000633:toxicity; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D016207:Cytokines; D037742:Nanotubes, Carbon; D017382:Reactive Oxygen Species