title: Oxidative damage to biological macromolecules in human bone marrow mesenchymal stromal cells labeled with various types of iron oxide nanoparticles.
abstract: The biological effects of several superparamagnetic iron oxide nanoparticles (SPIONs) varying in their surface coating were tested using human bone marrow mesenchymal stromal cells from two donors - hBMSCs-1 and hBMSCs-2. The measurements were performed at two intervals - after 72 h exposure to the nanoparticles and after an additional 72 h cell growth without nanoparticles. The dose of SPIONs used (15.4 μg Fe/ml) was selected as being sufficient for in vivo cell tracking using magnetic resonance imaging (MRI). Concerning cell viability and cell death, only the hBMSCs-2 seemed to be sensitive to the action of SPIONs. However, an increase of oxidative injury to lipids, proteins and DNA as a consequence of exposure to SPIONs was detected in cells from both donors. Particularly the levels of lipid peroxidation were high and increased further with time, regardless of the type of nanoparticle. Lowering intracellular label concentrations and authenticating oxidative stress levels using in vivo experiments are required to ensure the safety of SPIONs for biomedical applications.
authors.affiliation: Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic. novotna@biomed.cas.cz
authors.forename: Bozena
authors.initials: B
authors.lastname: Novotna
authors.forename: Pavla
authors.initials: P
authors.lastname: Jendelova
authors.forename: Miroslava
authors.initials: M
authors.lastname: Kapcalova
authors.forename: Pavel
authors.initials: P
authors.lastname: Rossner
authors.forename: Karolina
authors.initials: K
authors.lastname: Turnovcova
authors.forename: Yana
authors.initials: Y
authors.lastname: Bagryantseva
authors.forename: Michal
authors.initials: M
authors.lastname: Babic
authors.forename: Daniel
authors.initials: D
authors.lastname: Horak
authors.forename: Eva
authors.initials: E
authors.lastname: Sykova
mesh_terms: D016923:Cell Death / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002648:Child; D004249:DNA Damage / Q000187:drug effects; D005290:Ferric Compounds / Q000009:adverse effects*; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles / Q000009:adverse effects*; D059630:Mesenchymal Stem Cells / Q000187:drug effects*; D008875:Middle Aged; D018384:Oxidative Stress / Q000187:drug effects*; D011506:Proteins / Q000187:drug effects
chemical_list: D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; D011506:Proteins; C000499:ferric oxide