title: Inhibition of NF- κB activity by minor polar components of extra-virgin olive oil at gastric level.
abstract: The present work evaluates the effect of olive oil phenols on NF-κB activity in human gastric adenocarcinoma cells. The total phenol content was measured by the Folin Ciocalteu method, whereas the composition was assessed by LC-MS/MS analysis. Secoiridoids represented 71% and 83% of the Italian and Spanish extracts, respectively, phenol alcohols were in the range 9-13%. Ligustroside aglycone was the most abundant (37% and 46%, respectively, in the Italian and Spanish sample), and the concentration of flavonoids AP and LU was below 1%. Phenol extracts were assayed at 0.25-7.5 µg/mL, whereas single compounds were at 0.5-25 µM. Both the extracts inhibited the NF-κB driven transcription in a concentration-dependent manner: IC(50) for the Italian and the Spanish extract were 0.86 and 1.28 µg/mL, respectively. The IC(50) for individual compounds ranged from 4.5 to 13 µM. All the compounds under study inhibited nuclear translocation as well. The data suggest that consumption of extra-virgin olive oil may be beneficial for preventing the onset of gastric inflammation leading to more serious diseases.
authors.affiliation: Department of Pharmacological Sciences, Università degli Studi di Milano, Milano, Italy.
authors.forename: Enrico
authors.initials: E
authors.lastname: Sangiovanni
authors.forename: Elisa
authors.initials: E
authors.lastname: Colombo
authors.forename: Marco
authors.initials: M
authors.lastname: Fumagalli
authors.forename: Federico
authors.initials: F
authors.lastname: Abbiati
authors.forename: Donatella
authors.initials: D
authors.lastname: Caruso
authors.forename: Mario
authors.initials: M
authors.lastname: Dell'Agli
mesh_terms: D045744:Cell Line, Tumor; D002853:Chromatography, Liquid; D005419:Flavonoids / Q000032:analysis; D006801:Humans; D020128:Inhibitory Concentration 50; D039823:Iridoids / Q000032:analysis; D017705:Lignans / Q000032:analysis; D016328:NF-kappa B / Q000037:antagonists & inhibitors*; D000069463:Olive Oil; D010636:Phenols / Q000494:pharmacology*; D010938:Plant Oils / Q000737:chemistry* / Q000494:pharmacology*; D053719:Tandem Mass Spectrometry; D014158:Transcription, Genetic
chemical_list: D005419:Flavonoids; D039823:Iridoids; D017705:Lignans; D016328:NF-kappa B; D000069463:Olive Oil; D010636:Phenols; D010938:Plant Oils