title: Estimating intestinal mucosal permeation of compounds using Caco-2 cell monolayers.
abstract: Step-by-step protocols are provided in this unit for the measurement of apparent permeability coefficients of compounds using Caco-2 cell monolayers as an in vitro model of the intestinal mucosa. Procedures for culturing the cells and transmonolayer transport studies are also included. Critical issues for successfully estimating intestinal mucosal permeation of drugs are discussed. Step-by-step protocols are provided in this unit for the measurement of apparent permeability coefficients of compounds using.
authors.affiliation: University of Kansas, Lawrence, Kansas, USA.
authors.forename: J
authors.initials: J
authors.lastname: Gao
authors.forename: E D
authors.initials: ED
authors.lastname: Hugger
authors.forename: M S
authors.initials: MS
authors.lastname: Beck-Westermeyer
authors.forename: R T
authors.initials: RT
authors.lastname: Borchardt
mesh_terms: D001692:Biological Transport / Q000502:physiology; D018938:Caco-2 Cells; D002250:Carbon Radioisotopes; D002336:Carnosine / Q000493:pharmacokinetics*; D006801:Humans; D007408:Intestinal Absorption / Q000502:physiology*; D007413:Intestinal Mucosa / Q000378:metabolism*; D008353:Mannitol / Q000493:pharmacokinetics; D010539:Permeability
chemical_list: D002250:Carbon Radioisotopes; D008353:Mannitol; D002336:Carnosine