title: CKC: isolation of nucleic acids from a diversity of plants using CTAB and silica columns.
abstract: To assay for viruses in plant samples, we required a method for nucleic acid isolation that is rapid, simple, and applicable to the widest possible variety of plants. A protocol for isolation of total nucleic acid (TNA) was developed by combining common CTAB methods with silica spin columns. We report data on TNA purity and RNA quality from over 30 plant genera representing 25 families. Measurements showed that RNA is of high quality, and one-step RT-PCR was successfully performed on all samples. The protocol can be completed in less than 2 h.
authors.affiliation: USDA-ARS, United States National Arboretum, Floral and Nursery Plants Research Unit, B-010A, Beltsville, MD 20705, USA. david.henderson@ars.usda.gov
authors.forename: David C
authors.initials: DC
authors.lastname: Henderson
authors.forename: John
authors.initials: J
authors.lastname: Hammond
mesh_terms: D001709:Biotechnology / Q000295:instrumentation / Q000379:methods*; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry*; D009696:Nucleic Acids / Q000737:chemistry / Q000302:isolation & purification*; D010944:Plants / Q000737:chemistry* / Q000235:genetics; D019347:Potassium Acetate / Q000737:chemistry; D018749:RNA, Plant / Q000737:chemistry / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D002593:Cetrimonium Compounds; D009696:Nucleic Acids; D018749:RNA, Plant; D012822:Silicon Dioxide; D019347:Potassium Acetate; D000077286:Cetrimonium