title: Ultraviolet-visible light (UV-Vis)-reversible but fluorescence-irreversible chemosensor for copper in water and its application in living cells.
abstract: An ultraviolet-visible light (UV-Vis)-reversible but fluorescence-irreversible chemosensor was developed for the detection of copper. Coordination between the probe, 2-pyridylaldehyde fluorescein hydrazone (FHP), and Cu(2+) gave a reversible UV-Vis response, Storage of the probe-Cu complex resulted in hydrolytic cleavage of the N═C bond, which released the fluorophore (ring-opened fluorescein hydrazine) and gave irreversible fluorescence. Thus, FHP becomes a multifunctional chemosensor, and its reversibility can be controlled by the reaction time. Cu(2+) in living cells could be detected using FHP and general fluorescence methods.
authors.affiliation: Research Institute of Applied Cheemistry, Shanxi University, Taiyuan 030006, People's Republic of China.
authors.forename: Fang-Jun
authors.initials: FJ
authors.lastname: Huo
authors.forename: Cai-Xia
authors.initials: CX
authors.lastname: Yin
authors.forename: Yu-Tao
authors.initials: YT
authors.lastname: Yang
authors.forename: Jing
authors.initials: J
authors.lastname: Su
authors.forename: Jian-Bin
authors.initials: JB
authors.lastname: Chao
authors.forename: Dian-Sheng
authors.initials: DS
authors.lastname: Liu
mesh_terms: D056831:Coordination Complexes / Q000737:chemistry; D003300:Copper / Q000032:analysis*; D005456:Fluorescent Dyes / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D006834:Hydrazines / Q000737:chemistry; D008027:Light; D018613:Microscopy, Confocal; D013056:Spectrophotometry, Ultraviolet; D014466:Ultraviolet Rays; D014867:Water / Q000737:chemistry*
chemical_list: D056831:Coordination Complexes; D005456:Fluorescent Dyes; D006834:Hydrazines; D014867:Water; C029424:hydrazine; D003300:Copper