title: VEGF-C antisense oligoxydeonucleotide suppression of invasive ability of the A-549 lung carcinoma cell line.
abstract: OBJECTIVE
  Vascular endothelial growth factor C (VEGF-C), a member of the VEGF family, has been reported to promote angiogenesis and tumor cell growth. In this study, we analyzed inhibitory action of a VEGF-C antisense oligoxydeonucleotide (ASODN) on a lung carcinoma cell line A-549 and its invasive ability in vitro.
  
  
  METHODS
  Liposomes, liposome-mediated sense oligoxydeonucleotide (SODN) and ASODN were transfected into A-549 cells, with a blank control group. The expression of VEGF-C was examined by western blotting and invasive ability of cells was detected at four levels.
  
  
  RESULTS
  Lower expression of VEGF-C and lower invasive ability to recombinate basal membranes were apparent in the ASOND group (P<0.01). However, there were no significant differences among the other three groups (P>0.05). In the invasion assay, the number of transmembrane cells was significantly reduced in the ASODN group after 48 hours (58.6±9.2 P<0.01), but there was no variation among control, LIP and SOND groups (132.5±15.6, 129.7±16.1, 118.2±12.5, P>0.05).
  
  
  CONCLUSIONS
  VEGF-C ASODN can downregulate the expression of VEGF-C in cell lines and can obviously inhibit invasive ability in vitro.
authors.affiliation: Thoracic Department, Second Hospital of Shandong University, Jinan, China. pechuliang@126.com
authors.forename: Chuanliang
authors.initials: C
authors.lastname: Peng
authors.forename: Qifeng
authors.initials: Q
authors.lastname: Sun
authors.forename: Yunzhi
authors.initials: Y
authors.lastname: Fang
authors.forename: Bo
authors.initials: B
authors.lastname: Cong
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Zhao
mesh_terms: D002277:Carcinoma / Q000235:genetics* / Q000473:pathology / Q000628:therapy*; D045744:Cell Line, Tumor; D015536:Down-Regulation; D015316:Genetic Therapy; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology / Q000628:therapy*; D009361:Neoplasm Invasiveness; D016376:Oligonucleotides, Antisense / Q000008:administration & dosage* / Q000235:genetics*; D014162:Transfection / Q000379:methods; D042582:Vascular Endothelial Growth Factor C / Q000235:genetics*
chemical_list: D016376:Oligonucleotides, Antisense; D042582:Vascular Endothelial Growth Factor C