title: Determination of inflammatory and prominent proteomic changes in plasma and adipose tissue after high-intensity intermittent training in overweight and obese males.
abstract: This study aimed to determine whether 2 wk of high-intensity intermittent training (HIIT) altered inflammatory status in plasma and adipose tissue in overweight and obese males. Twelve participants [mean (SD): age 23.7 (5.2) yr, body mass 91.0 (8.0) kg, body mass index 29.1 (3.1) kg/m(2)] undertook six HIIT sessions over 2 wk. Resting blood and subcutaneous abdominal adipose tissue samples were collected and insulin sensitivity determined, pre- and posttraining. Inflammatory proteins were quantified in plasma and adipose tissue. There was a significant decrease in soluble interleukin-6 receptor (sIL-6R; P = 0.050), monocyte chemotactic protein-1 (MCP-1, P = 0.047), and adiponectin (P = 0.041) in plasma posttraining. Plasma IL-6, intercellular adhesion molecule-1 (ICAM-1), tumor necrosis factor-α (TNF-α), IL-10, and insulin sensitivity did not change. In adipose tissue, IL-6 significantly decreased (P = 0.036) and IL-6R increased (P = 0.037), while adiponectin tended to decrease (P = 0.056), with no change in ICAM-1 posttraining. TNF-α, MCP-1, and IL-10 were not detectable in adipose tissue. Adipose tissue homogenates were then resolved using one-dimensional gel electrophoresis, and major changes in the adipose tissue proteome, as a consequence of HIIT, were evaluated. This proteomic approach identified significant reductions in annexin A2 (P = 0.046) and fatty acid synthase (P = 0.016) as a response to HIIT. The present investigation suggests 2 wk of HIIT is sufficient to induce beneficial alterations in the resting inflammatory profile and adipose tissue proteome of an overweight and obese male cohort.
authors.affiliation: School of Sport, Exercise and Health Sciences, Loughborough University, Loughborough, Leicestershire, United Kingdom.
authors.forename: Melanie
authors.initials: M
authors.lastname: Leggate
authors.forename: Wayne G
authors.initials: WG
authors.lastname: Carter
authors.forename: Matthew J C
authors.initials: MJ
authors.lastname: Evans
authors.forename: Rebecca A
authors.initials: RA
authors.lastname: Vennard
authors.forename: Sarah
authors.initials: S
authors.lastname: Sribala-Sundaram
authors.forename: Myra A
authors.initials: MA
authors.lastname: Nimmo
mesh_terms: D052242:Adiponectin / Q000097:blood; D000273:Adipose Tissue / Q000378:metabolism*; D000293:Adolescent; D000328:Adult; D017306:Annexin A2 / Q000378:metabolism; D001642:Bicycling / Q000502:physiology*; D018932:Chemokine CCL2 / Q000097:blood; D015331:Cohort Studies; D015444:Exercise / Q000502:physiology*; D064429:Fatty Acid Synthases / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D009765:Obesity / Q000378:metabolism / Q000503:physiopathology*; D050177:Overweight / Q000378:metabolism / Q000503:physiopathology*; D040901:Proteomics; D019947:Receptors, Interleukin-6 / Q000097:blood; D055815:Young Adult
chemical_list: D052242:Adiponectin; D017306:Annexin A2; C111835:CCL2 protein, human; D018932:Chemokine CCL2; D015850:Interleukin-6; D019947:Receptors, Interleukin-6; D064429:Fatty Acid Synthases