title: Ethanol Does Not Promote MeIQx-initiated Rat Colon Carcinogenesis Based on Evidence from Analysis of a Colon Cancer Surrogate Marker.
abstract: Epidemiological studies suggest that alcohol consumption increases the risk of developing colorectal cancer. However, the data are confounded by numerous cosegregating variables. To cast further light on the relationships between alcohol intake and colon cancer development, 21-day-old male F344/DuCrj rats were fed 200 ppm 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx) in their diet for 8 weeks and doses of 0, 0.1, 0.3, 1, 3, 10 and 20% of ethanol in their drinking water ad libitum for 16 weeks thereafter. The rats were sacrificed after 24 weeks of experiment, and aberrant crypt foci (ACF), surrogate lesions for colon cancer, were examined under a light microscope at low magnification. Ethanol was found not to affect the ACF formation at any dose compared with the initiated-controls. Furthermore, ethanol did not alter colon epithelial cell proliferation. These data, obtained by analysis of a colon cancer surrogate marker lesion, indicate that ethanol lacks promotion activity for MeIQx-initiated rat colon carcinogenesis.
authors.forename: Masahiko
authors.initials: M
authors.lastname: Kushida
authors.forename: Hideki
authors.initials: H
authors.lastname: Wanibuchi
authors.forename: Min
authors.initials: M
authors.lastname: Wei
authors.forename: Anna
authors.initials: A
authors.lastname: Kakehashi
authors.forename: Keisuke
authors.initials: K
authors.lastname: Ozaki
authors.forename: Tokuo
authors.initials: T
authors.lastname: Sukata
authors.forename: Kaori
authors.initials: K
authors.lastname: Miyata
authors.forename: Keiko
authors.initials: K
authors.lastname: Ogata
authors.forename: Satoshi
authors.initials: S
authors.lastname: Uwagawa
authors.forename: Shoji
authors.initials: S
authors.lastname: Fukushima