title: Sol-gel derived nanoporous compositions for entrapping small molecules and their outlook toward aptamer screening.
abstract: This paper reports for the first time the application of sol-gel microarrays for immobilizing nonsoluble small chemicals (Bisphenol-A; BPA). Also, known problems of sol-gel adhesion to conventional microtiter well plate substrates are circumvented by anchoring the sol-gel microspots to a porous silion surface so-called, PS-SG chips. We confirmed low molecular weight chemical immobilization inside a sol-gel network using fluorescein. BPA and the BPA specific aptamer were utilized as a model pair to verify the affinity specific interaction in the PS-SG selection system. The aptamer interacted specifically with BPA in the sol-gel spots, as shown in microarrays forming the letters "L", "U", "N", and "D". Moreover, the bound aptamer was released by heat, recovered, and verified by gel electrophoresis. The developed PS-SG chip platform will be used for screening aptamers against numerous small molecules such as toxins, metabolites, or pesticide residues.
authors.affiliation: Department of Biomedical Engineering, Dongguk University, Seoul, Korea.
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Ahn
authors.forename: SangWook
authors.initials: S
authors.lastname: Lee
authors.forename: Minjoung
authors.initials: M
authors.lastname: Jo
authors.forename: Jeehye
authors.initials: J
authors.lastname: Kang
authors.forename: Eunkyung
authors.initials: E
authors.lastname: Kim
authors.forename: Ok Chan
authors.initials: OC
authors.lastname: Jeong
authors.forename: Thomas
authors.initials: T
authors.lastname: Laurell
authors.forename: Soyoun
authors.initials: S
authors.lastname: Kim
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001559:Benzhydryl Compounds; D004867:Equipment Design; D005782:Gels / Q000737:chemistry*; D046228:Microarray Analysis / Q000295:instrumentation*; D044367:Phase Transition; D010636:Phenols / Q000737:chemistry; D016062:Porosity; D000098826:Bisphenol A Compounds
chemical_list: D052157:Aptamers, Nucleotide; D001559:Benzhydryl Compounds; D005782:Gels; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds