title: Detection of aneuploidy by array comparative genomic hybridization using cell lines to mimic a mosaic trophectoderm biopsy.
abstract: OBJECTIVE
  To examine the effect of mosaicism in the array comparative genomic hybridization result during preimplantation genetic screening after blastocyst biopsy.
  
  
  METHODS
  Experimental study.
  
  
  METHODS
  University laboratory. MATERIAL(S): Epithelial cell lines.
  
  
  METHODS
  Mixing of euploid and aneuploid cells to create mosaic trophectoderm and blastocyst models.
  
  
  METHODS
  The level of aneuploidy in samples with different ratios of aneuploid cells was measured after array comparative genomic hybridization.
  
  
  RESULTS
  A shift from normality was present when the level of aneuploid cells in the sample was >25%. Aneuploidy could be confidently called when the level of aneuploid cells was >50%.
  
  
  CONCLUSIONS
  This study determined that aneuploidy in mosaic samples can be detected by array comparative genomic hybridization and that the result may also indicate the proportion of the aneuploid cells present in the sample.
authors.affiliation: UCL Centre for PGD, Institute for Women's Health, University College London, London, United Kingdom. t.mamas@ucl.ac.uk
authors.forename: Thalia
authors.initials: T
authors.lastname: Mamas
authors.forename: Anthony
authors.initials: A
authors.lastname: Gordon
authors.forename: Anthony
authors.initials: A
authors.lastname: Brown
authors.forename: Joyce
authors.initials: J
authors.lastname: Harper
authors.forename: Sioban
authors.initials: S
authors.lastname: Sengupta
mesh_terms: D000782:Aneuploidy; D001706:Biopsy; D002460:Cell Line; D002877:Chromosomes, Human; D055028:Comparative Genomic Hybridization; D004475:Ectoderm / Q000473:pathology*; D005260:Female; D005820:Genetic Testing; D006801:Humans; D009030:Mosaicism; D011237:Predictive Value of Tests; D019836:Preimplantation Diagnosis / Q000379:methods*; D014327:Trophoblasts / Q000473:pathology*