title: Hog Charm II tetracycline test screening results compared with a liquid chromatography tandem mass spectrometry 10-μg/kg method.
abstract: Pork tissue samples that tested positive and negative by the Charm II tetracycline test screening method in the slaughter plant laboratory were tested with the modified AOAC International liquid chromatography tandem mass spectrometry (LC-MS-MS) method 995.09 to determine the predictive  value of the screening method at detecting total tetracyclines at 10 μg/kg of tissue, in compliance with Russian import regulations. There were 218 presumptive-positive tetracycline samples of 4,195 randomly tested hogs. Of these screening test positive samples, 83% (182) were positive,  >10 μg/kg by LC-MS-MS; 12.8% (28) were false violative, greater than limit of detection (LOD) but <10 μg/kg; and 4.2% (8) were not detected at the LC-MS-MS LOD. The 36 false-violative and not-detected samples represent 1% of the total samples screened. Twenty-seven of 30 randomly  selected tetracycline screening negative samples tested below the LC-MS-MS LOD, and 3 samples tested <3 μg/kg chlortetracycline. Results indicate that the Charm II tetracycline test is effective at predicting hogs containing >10 μg/kg total tetracyclines in compliance with Russian  import regulations.
authors.affiliation: Charm Sciences, Inc., Lawrence, MA 01843, USA. bobs@charm.com
authors.forename: Robert
authors.initials: R
authors.lastname: Salter
authors.forename: Steven
authors.initials: S
authors.lastname: Holmes
authors.forename: David
authors.initials: D
authors.lastname: Legg
authors.forename: Joel
authors.initials: J
authors.lastname: Coble
authors.forename: Bruce
authors.initials: B
authors.lastname: George
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification; D002853:Chromatography, Liquid / Q000379:methods; D004350:Drug Residues / Q000032:analysis*; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000379:methods; D013752:Tetracycline / Q000302:isolation & purification*
chemical_list: D000900:Anti-Bacterial Agents; D013752:Tetracycline