title: Plasmid-mediate transfer of FLO1 into industrial Saccharomyces cerevisiae PE-2 strain creates a strain useful for repeat-batch fermentations involving flocculation-sedimentation.
abstract: The flocculation gene FLO1 was transferred into the robust industrial strain Saccharomyces cerevisiae PE-2 by the lithium acetate method. The recombinant strain showed a fermentation performance similar to that of the parental strain. In 10 repeat-batch cultivations in VHG medium with 345 g glucose/L and cell recycling by flocculation-sedimentation, an average final ethanol concentration of 142 g/L and an ethanol productivity of 2.86 g/L/h were achieved. Due to the flocculent nature of the recombinant strain it is possible to reduce the ethanol production cost because of lower centrifugation and distillation costs.
authors.affiliation: IBB - Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal. dgg.ebiol@gmail.com
authors.forename: Daniel G
authors.initials: DG
authors.lastname: Gomes
authors.forename: Pedro M R
authors.initials: PM
authors.lastname: Guimarães
authors.forename: Francisco B
authors.initials: FB
authors.lastname: Pereira
authors.forename: José A
authors.initials: JA
authors.lastname: Teixeira
authors.forename: Lucília
authors.initials: L
authors.lastname: Domingues
mesh_terms: D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005427:Flocculation; D018014:Gene Transfer Techniques; D007218:Industrial Microbiology / Q000379:methods*; D037241:Mannose-Binding Lectins / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D013045:Species Specificity; D014170:Transformation, Genetic / Q000235:genetics*
chemical_list: C087578:FLO1 protein, S cerevisiae; D037241:Mannose-Binding Lectins; D029701:Saccharomyces cerevisiae Proteins; D000431:Ethanol