title: Latent Kaposi's sarcoma-associated herpesvirus infection of monocytes downregulates expression of adaptive immune response costimulatory receptors and proinflammatory cytokines.
abstract: Kaposi's sarcoma-associated herpesvirus (KSHV) infection is associated with the development of Kaposi's sarcoma, primary effusion lymphoma, and multicentric Castleman's disease. We report the establishment of a monocytic cell line latently infected with KSHV (KSHV-THP-1). We profiled viral and cytokine gene expression in the KSHV-THP-1 cells compared to that in uninfected THP-1 cells and found that several genes involved in the host immune response were downregulated during latent infection, including genes for CD80, CD86, and the cytokines tumor necrosis factor alpha (TNF-α) and interleukin-1β (IL-1β). Thus, KSHV minimizes its immunological signature by suppressing key immune response factors, enabling persistent infection and evasion from host detection.
authors.affiliation: Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
authors.forename: Sean M
authors.initials: SM
authors.lastname: Gregory
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.forename: John A
authors.initials: JA
authors.lastname: West
authors.forename: Dirk P
authors.initials: DP
authors.lastname: Dittmer
authors.forename: Blossom
authors.initials: B
authors.lastname: Damania
mesh_terms: D000818:Animals; D002460:Cell Line; D016207:Cytokines / Q000235:genetics* / Q000276:immunology; D015536:Down-Regulation; D015967:Gene Expression Regulation, Viral; D019288:Herpesvirus 8, Human / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D006801:Humans; D009000:Monocytes / Q000276:immunology* / Q000821:virology; D011971:Receptors, Immunologic / Q000235:genetics* / Q000276:immunology; D012514:Sarcoma, Kaposi / Q000235:genetics* / Q000276:immunology / Q000821:virology; D017735:Virus Latency
chemical_list: D016207:Cytokines; D011971:Receptors, Immunologic