title: Updates on the genetic variations of norovirus in sporadic gastroenteritis in Chungnam Korea, 2009-2010.
abstract: Previously, we explored the epidemic pattern and molecular characterization of noroviruses (NoVs) isolated in Chungnam, Korea in 2008, and the present study extended these observations to 2009 and 2010. In Korea, NoVs showed the seasonal prevalence from late fall to spring, and widely detected in preschool children and peoples over 60 years of age. Epidemiological pattern of NoV was similar in 2008 and in 2010, but pattern in 2009 was affected by pandemic influenza A/H1N1 2009 virus. NoV-positive samples were subjected to sequence determination of the capsid gene region, which resolved the isolated NoVs into five GI (2, 6, 7, 9 and 10) and eleven GII genotypes (1, 2, 3, 4, 6, 7, 8, 12, 13, 16 and 17). The most prevalent genotype was GII.4 and occupied 130 out of 211 NoV isolates (61.6%). Comparison of NoV GII.4 of prevalent genotype in these periods with reference strains of the same genotype was conducted to genetic analysis by a phylogenetic tree. The NoV GII.4 strains were segregated into seven distinct genetic groups, which are supported by high bootstrap values and previously reported clusters. All Korean NoV GII.4 strains belonged to either VI cluster or VII cluster. The divergence of nucleotide sequences within VI and VII intra-clusters was > 3.9% and > 3.5%, respectively. The "Chungnam(06-117)/2010" strain which was isolated in June 2010 was a variant that did not belong to cluster VI or VII and showed 5.8-8.2%, 6.2-8.1% nucleotide divergence with cluster VI and VII, respectively.
authors.affiliation: Chungcheongnam-Do Institute of Health and Environmental Research, Daejeon, Korea.
authors.forename: KwiSung
authors.initials: K
authors.lastname: Park
authors.forename: SangGu
authors.initials: S
authors.lastname: Yeo
authors.forename: HyeSook
authors.initials: H
authors.lastname: Jeong
authors.forename: KyoungAh
authors.initials: K
authors.lastname: Baek
authors.forename: DongUk
authors.initials: D
authors.lastname: Kim
authors.forename: MyoungHee
authors.initials: M
authors.lastname: Shin
authors.forename: JaeHyoung
authors.initials: J
authors.lastname: Song
authors.forename: SooJin
authors.initials: S
authors.lastname: Lee
authors.forename: YoungJin
authors.initials: Y
authors.lastname: Choi
authors.forename: JoonSoo
authors.initials: J
authors.lastname: Park
authors.forename: SungChan
authors.initials: S
authors.lastname: Cho
authors.forename: DooSung
authors.initials: D
authors.lastname: Cheon
mesh_terms: D000368:Aged; D017250:Caliciviridae Infections / Q000453:epidemiology* / Q000821:virology*; D036022:Capsid Proteins / Q000235:genetics; D002675:Child, Preschool; D005260:Female; D005759:Gastroenteritis / Q000453:epidemiology* / Q000821:virology*; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D029322:Norovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D056910:Republic of Korea / Q000453:epidemiology
chemical_list: D036022:Capsid Proteins