title: The identification of Pi50(t), a new member of the rice blast resistance Pi2/Pi9 multigene family.
abstract: The deployment of broad-spectrum resistance genes is the most effective and economic means of controlling blast in rice. The cultivar Er-Ba-Zhan (EBZ) is a widely used donor of blast resistance in South China, with many cultivars derived from it displaying broad-spectrum resistance against blast. Mapping in a set of recombinant inbred lines bred from the cross between EBZ and the highly blast-susceptible cultivar Liangjiangxintuanheigu (LTH) identified in EBZ a blast resistance gene on each of chromosomes 1 (Pish), 6 (Pi2/Pi9) and 12 (Pita/Pita-2). The resistance spectrum and race specificity of the allele at Pi2/Pi9 were both different from those present in other known Pi2/Pi9 carriers. Fine-scale mapping based on a large number of susceptible EBZ × LTH F(2) and EBZ × LTH BC(1)F(2) segregants placed the gene within a 53-kb segment, which includes Pi2/Pi9. Sequence comparisons of the LRR motifs of the four functional NBS-LRR genes within Pi2/Pi9 revealed that the EBZ allele is distinct from other known Pi2/Pi9 alleles. As a result, the gene has been given the designation Pi50(t).
authors.affiliation: Laboratory of Plant Resistance and Genetics, College of Resources and Environmental Sciences, South China Agricultural University, Guangzhou 510642, China. zhuxy@gdppri.com
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Zhu
authors.forename: Shen
authors.initials: S
authors.lastname: Chen
authors.forename: Jianyuan
authors.initials: J
authors.lastname: Yang
authors.forename: Shaochuan
authors.initials: S
authors.lastname: Zhou
authors.forename: Liexian
authors.initials: L
authors.lastname: Zeng
authors.forename: Jingluan
authors.initials: J
authors.lastname: Han
authors.forename: Jing
authors.initials: J
authors.lastname: Su
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Pan
mesh_terms: D001947:Breeding; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics; D003433:Crosses, Genetic; D060467:Disease Resistance / Q000235:genetics*; D017343:Genes, Plant; D005838:Genotype; D020082:Magnaporthe / Q000472:pathogenicity*; D005810:Multigene Family; D012275:Oryza / Q000235:genetics* / Q000382:microbiology; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics
chemical_list: D010940:Plant Proteins