title: Enhanced osteoblast responses to poly(methyl methacrylate)/hydroxyapatite electrospun nanocomposites for bone tissue engineering.
abstract: Hydroxyapatite (HA)-containing polymers have been proposed for improving the biological properties of bone cements. Poly(methyl methacrylate) (PMMA) has long been used to secure orthopedic implants to skeletal bones. The aim of this study was to determine whether the incorporation of HA nanoparticles into the PMMA nanofibrous scaffolds enhances the biological functions of osteoblasts. The number of osteoblasts adhered and proliferated on the PMMA/HA nanofibrous scaffolds was significantly larger than that on the PMMA alone. The cytoskeletal organization and alkaline phosphatase (ALP) activity of the osteoblasts on the PMMA/HA nanofibrous scaffolds were clearly higher than that on the PMMA control. The amount of calcium ions released from 20 wt% HA-containing PMMA nanofibrous scaffolds (PMMA/HA20) was much higher than that released from 10 wt% HA-containing PMMA nanofibrous scaffolds (PMMA/HA10) (HA, 10 wt%). These findings suggested that osteoblast differentiation was accelerated by the incorporation of HA into the PMMA nanofibrous scaffolds. Therefore, the incorporation of HA into the PMMA nanofibrous scaffolds could be a useful method. This can be used for providing PMMA scaffolds with enhanced osteogenic properties.
authors.affiliation: Department of Polymer Science and Engineering , Kyungpook National University, Daegu, South Korea.
authors.forename: Zhi-Cai
authors.initials: ZC
authors.lastname: Xing
authors.forename: Seung-Jin
authors.initials: SJ
authors.lastname: Han
authors.forename: Yong-Suk
authors.initials: YS
authors.lastname: Shin
authors.forename: Tae-Hyung
authors.initials: TH
authors.lastname: Koo
authors.forename: Sungmo
authors.initials: S
authors.lastname: Moon
authors.forename: Yongsoo
authors.initials: Y
authors.lastname: Jeong
authors.forename: Inn-Kyu
authors.initials: IK
authors.lastname: Kang
mesh_terms: D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D001842:Bone and Bones / Q000378:metabolism; D002448:Cell Adhesion; D002454:Cell Differentiation; D017886:Durapatite / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D057139:Nanofibers / Q000737:chemistry / Q000648:ultrastructure; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D019904:Polymethyl Methacrylate / Q000737:chemistry / Q000378:metabolism*; D013718:Tensile Strength; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D019904:Polymethyl Methacrylate; D017886:Durapatite; D000469:Alkaline Phosphatase