title: Proteomics approaches in the quest of kidney disease biomarkers.
abstract: Proteomics refers to a group of analytical techniques for high throughput protein analysis, providing evidence for protein expression levels, subcellular localization, post-translational modifications and molecular interactions. As such, proteomics has contributed largely to our knowledge regarding molecular mechanisms underlying health and disease and pinpointed potential disease biomarkers. The scope of this review is to briefly introduce the principles of major proteomics techniques employed in biological research, including novel quantitative and molecular imaging mass spectrometry-based platforms. A few examples from the application of these techniques in biomarker discovery for kidney diseases are also provided.
authors.affiliation: Biotechnology Division, Biomedical Research Foundation,  Academy of Athens, Greece.
authors.forename: M
authors.initials: M
authors.lastname: Frantzi
authors.forename: V
authors.initials: V
authors.lastname: Bitsika
authors.forename: A
authors.initials: A
authors.lastname: Charonis
authors.forename: A
authors.initials: A
authors.lastname: Vlahou
mesh_terms: D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D035843:Biomedical Research / Q000639:trends; D006801:Humans; D007674:Kidney Diseases / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods; D011499:Protein Processing, Post-Translational; D020543:Proteome / Q000032:analysis / Q000378:metabolism; D040901:Proteomics / Q000379:methods / Q000639:trends
chemical_list: D015415:Biomarkers; D020543:Proteome