title: Highly efficient quenching of electrochemiluminescence from CdS nanocrystal film based on biocatalytic deposition.
abstract: Aiming to find an alternative electrochemiluminescence (ECL) quenching route of high efficiency, biocatalytic precipitation (BCP) was firstly coupled with ECL for investigating its insulating effect on ECL. Experimental data revealed that an insulating layer could be formed via BCP onto the electrode surface, inhibiting the reaction between the coreactant and luminophore and thereby impairing the ECL emission dramatically. Since the extent of insulating effect associated strongly with analyte concentration, a new ECL biosensor was successfully realized. In a model horseradish peroxidase (HRP)-based system, the fabricated biosensor possessed high sensitivity and wide linear range from 1.0 × 10(-10)M to 1.0 × 10(-6)M for H(2)O(2) determination with a detection limit of 4 × 10(-11)M (S/N=3). This method has great potential in extending the application of ECL biosensor for various bioanalytes.
authors.affiliation: State Key Lab of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Zhao
authors.forename: Chun-Yuan
authors.initials: CY
authors.lastname: Tian
authors.forename: Jing-Juan
authors.initials: JJ
authors.lastname: Xu
authors.forename: Hong-Yuan
authors.initials: HY
authors.lastname: Chen
mesh_terms: D015097:3-Mercaptopropionic Acid / Q000737:chemistry; D055162:Biocatalysis; D015374:Biosensing Techniques; D019187:Cadmium Compounds / Q000737:chemistry*; D004563:Electrochemistry; D004566:Electrodes; D006735:Horseradish Peroxidase / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000032:analysis*; D057230:Limit of Detection; D008163:Luminescent Measurements; D018625:Microscopy, Atomic Force; D053758:Nanoparticles; D045663:Quantum Dots; D012996:Solutions; D013440:Sulfides / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
chemical_list: D019187:Cadmium Compounds; D012996:Solutions; D013440:Sulfides; C034939:cadmium sulfide; D014867:Water; D015097:3-Mercaptopropionic Acid; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase