title: Maintenance of rat hepatocytes under inflammation by coculture with human orbital fat-derived stem cells.
abstract: Preservation of hepatocyte functions in vitro will undoubtedly help the management of acute liver failure. The coculture system may be able to prevent functional decline of hepatocytes. It has already been shown that hepatocytes, when cocultured with bone marrow mesenchymal stem cells, could undergo long-term culture in vitro without loss of functions. In this study, human orbital fat-derived stem cells were isolated and cocultured with rat hepatocytes. When treated with serum from an acute liver failure patient, rat hepatocyte monoculture showed reduction of cell viability and loss of liver-specific functions. However, rat hepatocytes in the coculture system were still able to secret albumin and synthesize urea. IL-6 was significantly elevated in the coculture of rat hepatocyte with orbital fat-derived stem cells, and it might be the key immunoregulator which protects rat hepatocytes against inflammation. Our data confirmed that orbital fat-derived stem cells, or other adipose tissue-derived stem cells, are an ideal candidate to support rat hepatocyte functions in vitro.
authors.affiliation: Department of Infectious Disease, Southwest Hospital Affiliated to Third Military Medical University, Chongqing 400038, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Chen
authors.forename: Shichang
authors.initials: S
authors.lastname: Zhang
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000418:Albumins / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation; D002470:Cell Survival; D018920:Coculture Techniques; D051544:Cytochrome P-450 CYP3A / Q000378:metabolism; D022781:Hepatocytes / Q000166:cytology*; D006801:Humans; D007249:Inflammation / Q000378:metabolism / Q000473:pathology; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D017114:Liver Failure, Acute / Q000097:blood; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D014508:Urea / Q000378:metabolism
chemical_list: D000418:Albumins; D015850:Interleukin-6; D014508:Urea; D051544:Cytochrome P-450 CYP3A