title: [Evaluation of biological effects of cobalt-nanocomposites with the use of biochemical markers of bivalve mollusk Anodonta cygnea].
abstract: Intensive implementation of nanomaterials requires development of novel methods for evaluation of their potential ecotoxicity. The aim of our study was to identify specific characteristics of the effect of cobalt-nanocomposite (Co-NC) on the molecular stress-responsive system in the digestive gland of bivalve mollusk Anodonta cygnea. Nanocomposite was synthesized by mixing alcohol solution of copolymer N-vinylpirrolidone, 5-(tret-butylperoxy)-5-methyl-1-hexene-3-yne and dimethylaminoethylmetacrylate and cobalt (II) chloride. After 14 days of the mollusk exposure in the presence of Co-NC, CoCl, or corresponding polymer substance it was shown that the Co-NC, in contrast to other agents, does not cause an oxidative stress due to the superoxide dismutase activity, metallotioneins (MTs) level, glutathione redox index and oxyradical production. Multivariate analysis confirmed specific features of the Co-NC's effect related to an enhanced expression of MTs, while CoCl2 activated lactate dehydrogenate and oxyradical production, and polymer substance enhanced glutathione transferase activity.
authors.forename: G I
authors.initials: GI
authors.lastname: Fal'fushins'ka
authors.forename: L L
authors.initials: LL
authors.lastname: Gnatyshina
authors.forename: O B
authors.initials: OB
authors.lastname: Stoliar
authors.forename: N Ie
authors.initials: NIe
authors.lastname: Mitina
authors.forename: O S
authors.initials: OS
authors.lastname: Zaichenko
authors.forename: Ie Z
authors.initials: IeZ
authors.lastname: Filiak
authors.forename: R S
authors.initials: RS
authors.lastname: Stoĭka
mesh_terms: D000818:Animals; D049877:Anodonta / Q000187:drug effects* / Q000502:physiology; D015415:Biomarkers / Q000378:metabolism*; D003035:Cobalt / Q000737:chemistry / Q000633:toxicity*; D004064:Digestive System / Q000187:drug effects* / Q000378:metabolism; D005021:Ethylamines / Q000737:chemistry; D005609:Free Radicals / Q000378:metabolism; D005978:Glutathione / Q000032:analysis; D005982:Glutathione Transferase / Q000032:analysis; D008668:Metallothionein / Q000032:analysis; D008689:Methacrylates / Q000737:chemistry; D015999:Multivariate Analysis; D053761:Nanocomposites / Q000737:chemistry / Q000633:toxicity*; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D011760:Pyrrolidinones / Q000737:chemistry; D013482:Superoxide Dismutase / Q000032:analysis; D014876:Water Pollution
chemical_list: D015415:Biomarkers; D005021:Ethylamines; D005609:Free Radicals; D008689:Methacrylates; D011760:Pyrrolidinones; C514459:aminoethyl methacrylate; D003035:Cobalt; C042670:N-vinyl-2-pyrrolidinone; D008668:Metallothionein; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D005978:Glutathione