title: Visualizing biochemical activities in living cells through chemistry.
abstract: The development of molecular probes to visualize cellular processes is an important challenge in chemical biology. One possibility to create such cellular indicators is based on the selective labeling of proteins with synthetic probes in living cells. Over the last years, our laboratory  has developed different labeling approaches for monitoring protein activity and for localizing synthetic probes inside living cells. In this article, we review two of these labeling approaches, the SNAP-tag and CLIP-tag technologies, and their use for studying cellular processes.
authors.affiliation: Institute of Chemical Sciences and Engineering, NCCR Chemical Biology, École Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland.
authors.forename: Luc
authors.initials: L
authors.lastname: Reymond
authors.forename: Gražvydas
authors.initials: G
authors.lastname: Lukinavičius
authors.forename: Keitaro
authors.initials: K
authors.lastname: Umezawa
authors.forename: Damien
authors.initials: D
authors.lastname: Maurel
authors.forename: Matthias A
authors.initials: MA
authors.lastname: Brun
authors.forename: Anastasiya
authors.initials: A
authors.lastname: Masharina
authors.forename: Karolina
authors.initials: K
authors.lastname: Bojkowska
authors.forename: Birgit
authors.initials: B
authors.lastname: Mollwitz
authors.forename: Alberto
authors.initials: A
authors.lastname: Schena
authors.forename: Rudolf
authors.initials: R
authors.lastname: Griss
authors.forename: Kai
authors.initials: K
authors.lastname: Johnsson
mesh_terms: D002118:Calcium / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; D011506:Proteins; D002118:Calcium