title: Detection and characterisation of two novel vitiviruses infecting Actinidia.
abstract: Two co-infecting novel vitiviruses from Actinidia chinensis were identified from mechanically inoculated Nicotiana occidentalis. Both virus genomes were sequenced and share 64% nucleotide identity. Their overall structure is typical of vitiviruses, with five open reading frames (ORFs) and a polyadenylated 3' end. Open reading frame 4 (ORF4) encodes the coat protein, the most conserved gene of the vitiviruses, in which they share 75% amino acid identity, 61-68% with grapevine virus B, 55-59% with grapevine virus A, and 37-42% with grapevine virus E. Based on the molecular criteria for species demarcation in the family Betaflexiviridae, these are two novel viruses, tentatively named Actinidia virus A and Actinidia virus B.
authors.affiliation: The New Zealand Institute for Plant and Food Research Ltd., Auckland, New Zealand. arnaud.blouin@plantandfood.co.nz
authors.forename: Arnaud G
authors.initials: AG
authors.lastname: Blouin
authors.forename: Ramesh R
authors.initials: RR
authors.lastname: Chavan
authors.forename: Michael N
authors.initials: MN
authors.lastname: Pearson
authors.forename: Robin M
authors.initials: RM
authors.lastname: MacDiarmid
authors.forename: Daniel
authors.initials: D
authors.lastname: Cohen
mesh_terms: D029042:Actinidia / Q000821:virology*; D060085:Coinfection; D052677:Flexiviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D023061:Gene Order; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010935:Plant Diseases / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014026:Nicotiana / Q000821:virology
chemical_list: D012367:RNA, Viral