title: Improved clonality detection in Hodgkin lymphoma using the BIOMED-2-based heavy and kappa chain assay: a paraffin-embedded tissue study.
abstract: OBJECTIVE
  Although BIOMED-2 polymerase chain reaction (PCR) standardization protocols allow clonality detection in nearly 100% of non-Hodgkin B cell lymphomas, they have not been widely validated for Hodgkin lymphoma (HL). Our aim was to assess BIOMED-2 protocol sensitivity when using non-microdissected, formalin-fixed, paraffin-embedded (FFPE) tissue from HL cases.
  
  
  RESULTS
  We studied 69 consecutive HL cases, of which 61 corresponded to classic HL (cHL) and eight to nodular lymphocyte-predominant HL (NLPHL). CD30-positive cell numbers (<10, 10-25 or >25 per ×200 field), background CD20-positive cell density (low or high) and tumour cell immunophenotype were evaluated. IGH and IGK clonality was assessed on FFPE tissue following BIOMED-2 protocols. Of the 58 assessable cHL cases, 15 (25.9%) exhibited IGH and/or IGK clonality; IGH clonality was shown by nine (15.5%) and IGK clonality by 12 (20.7%). Clonality detection rates in cHL improved as CD30-positive Reed-Sternberg (RS) cell density increased and CD20-positive B cell density decreased, although these correlations did not reach statistical significance. Of the eight NLPHL cases studied, none showed clonal rearrangement.
  
  
  CONCLUSIONS
  Combined study of IGH and IGK rearrangement according to BIOMED-2 protocols improves clonality detection rate (up to 25% of cases) in HL, even when working on non-microdissected FFPE tissue.
authors.affiliation: Department of Pathology, Hospital Germans Trias i Pujol, Badalona, Catalonia, Spain. gustavotapiam@hotmail.com
authors.forename: Gustavo
authors.initials: G
authors.lastname: Tapia
authors.forename: Carolina
authors.initials: C
authors.lastname: Sanz
authors.forename: José L
authors.initials: JL
authors.lastname: Mate
authors.forename: Ana M
authors.initials: AM
authors.lastname: Muñoz-Mármol
authors.forename: Aurelio
authors.initials: A
authors.lastname: Ariza
mesh_terms: D002999:Clone Cells / Q000276:immunology / Q000473:pathology; D015322:Gene Rearrangement, B-Lymphocyte / Q000235:genetics*; D006689:Hodgkin Disease / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D016612:Paraffin Embedding / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards
chemical_list: D007143:Immunoglobulin Heavy Chains; D007145:Immunoglobulin kappa-Chains