title: From climate change to molecular response: redox proteomics of ozone-induced responses in soybean.
abstract: • Ozone (O₃) causes significant agricultural losses, with soybean (Glycine max) being highly sensitive to this oxidant. Here we assess the effect of elevated seasonal O₃ exposure on the total and redox proteomes of soybean. • To understand the molecular responses to O₃ exposure, soybean grown at the Soybean Free Air Concentration Enrichment facility under ambient (37 ppb), moderate (58 ppb), and high (116 ppb) O₃ concentrations was examined by redox-sensitive thiol labeling, mass spectrometry, and targeted enzyme assays. • Proteomic analysis of soybean leaf tissue exposed to high O₃ concentrations reveals widespread changes. In the high-O₃ treatment leaf, 35 proteins increased up to fivefold in abundance, 22 proteins showed up to fivefold higher oxidation, and 22 proteins increased in both abundance and oxidation. These changes occurred in carbon metabolism, photosynthesis, amino acid synthesis, flavonoid and isoprenoid biosynthesis, signaling and homeostasis, and antioxidant pathways. • This study shows that seasonal O₃ exposure in soybean alters the abundance and oxidation state of redox-sensitive multiple proteins and that these changes reflect a combination of damage effects and adaptive responses that influence a wide range of metabolic processes, which in some cases may help mitigate oxidative stress.
authors.affiliation: Department of Biology, Washington University, One Brookings Drive, Campus Box 1137, St. Louis, MO 63130, USA.
authors.forename: Ashley
authors.initials: A
authors.lastname: Galant
authors.affiliation: Department of Plant Biology, 1201 West Gregory Drive, MC-051, University of Illinois, Urbana-Champaign, Urbana, IL 61801, USA.
authors.forename: Robert P
authors.initials: RP
authors.lastname: Koester
authors.affiliation: Department of Plant Biology, 1201 West Gregory Drive, MC-051, University of Illinois, Urbana-Champaign, Urbana, IL 61801, USA.|USDA-ARS Global Change and Photosynthesis Research Unit, 1201 West Gregory Drive, MC-051, Urbana, IL 61801, USA.
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Ainsworth
authors.affiliation: Donald Danforth Plant Science Center, 975 North Warson Road, St. Louis, MO 63132, USA.
authors.forename: Leslie M
authors.initials: LM
authors.lastname: Hicks
authors.affiliation: Department of Biology, Washington University, One Brookings Drive, Campus Box 1137, St. Louis, MO 63130, USA.
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Jez
mesh_terms: D057231:Climate Change; D015180:Electrophoresis, Gel, Two-Dimensional; D015151:Immunoblotting; D010084:Oxidation-Reduction / Q000187:drug effects; D010126:Ozone / Q000494:pharmacology*; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism; D020543:Proteome / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D012273:Ribulose-Bisphosphate Carboxylase / Q000378:metabolism; D013025:Glycine max / Q000187:drug effects* / Q000378:metabolism*; D013194:Staining and Labeling
chemical_list: D010940:Plant Proteins; D020543:Proteome; D010126:Ozone; D012273:Ribulose-Bisphosphate Carboxylase