title: Antioxidant activity of the methanolic extract of the newly generated vegetable, baemuchae (xBrassicoraphanus).
abstract: We investigated the radical scavenging activity and protective effects of the methanol extract (ME) and ferulic acid (FA) from baemuchae (xBrassicoraphanus) against oxidative stress in vitro and on a cellular system using LLC-PK1 renal epithelial cells. In vitro, ME and FA showed dose-dependent radical scavenging activity against 1,1-diphenyl-2-picrylhydrazyl and hydroxyl radicals. LLC-PK1 cells displayed decreased viability in response to nitric oxide-induced oxidative stress. However, ME and FA significantly increased LLC-PK1 cell viability in a dose-dependent manner. Based on HPLC/UV analysis, the FA content was higher in the shoot of baemuchae than in the root. The results of the present study suggest that baemuchae may be a promising source of antioxidants against oxidative stress induced by free radicals.
authors.affiliation: Department of Integrative Plant Science, Chung-Ang University, Anseong 456-756, Republic of Korea.
authors.forename: So-Youn
authors.initials: SY
authors.lastname: Mok
authors.forename: Mi Jin
authors.initials: MJ
authors.lastname: Choi
authors.forename: Jongkee
authors.initials: J
authors.lastname: Kim
authors.forename: Eun Ju
authors.initials: EJ
authors.lastname: Cho
authors.forename: Sanghyun
authors.initials: S
authors.lastname: Lee
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001937:Brassica / Q000737:chemistry*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D018374:LLC-PK1 Cells; D009682:Magnetic Resonance Spectroscopy; D000432:Methanol / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D013056:Spectrophotometry, Ultraviolet; D013552:Swine
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D000432:Methanol