title: Expression of WNT-5a and ROR2 correlates with disease severity in osteosarcoma.
abstract: Osteosarcoma, a common malignancy primarily affecting children, generally has a poor prognosis. Novel diagnostic, prognostic and therapeutic markers are required to ameliorate the negative outcomes of this disease. We investigated two potential markers, WNT-5a and ROR2, which are hypothesized to dysregulate WNT signaling pathways to promote tumorigenesis in other types of cancer. We investigated WNT-5a and ROR2 expression using immunohistochemistry in 42 osteosarcoma and 12 osteochondroma specimens, and compared the expression of these proteins with one another as well as with clinicopathological parameters. WNT-5a was detected in 34/42 (81.0%) cases and ROR2 was detected in 31/42 (73.8%) cases, significantly higher than in osteochondroma (16.7 and 25.0%, respectively; both P<0.05). Expression of these proteins was positively correlated (r=0.552, P<0.05). Furthermore, expression of WNT-5a and ROR2 was both correlated with Enneking surgical stage and tumor metastasis (P<0.05), but not with patient gender, age or pathological type. Thus, WNT-5a and ROR2 were more highly expressed in more severe disease states, and therefore may play a coordinated role in the occurrence and progression of osteosarcoma.
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital, Soochow University, Suzhou, Jiangsu 215006, PR China.
authors.forename: Bei-Ji
authors.initials: BJ
authors.lastname: Lu
authors.forename: Yun-Qing
authors.initials: YQ
authors.lastname: Wang
authors.forename: Xue-Jie
authors.initials: XJ
authors.lastname: Wei
authors.forename: Liang-Qun
authors.initials: LQ
authors.lastname: Rong
authors.forename: Dong
authors.initials: D
authors.lastname: Wei
authors.forename: Chang-Ming
authors.initials: CM
authors.lastname: Yan
authors.forename: Deng-Jie
authors.initials: DJ
authors.lastname: Wang
authors.forename: Jun-Ying
authors.initials: JY
authors.lastname: Sun
mesh_terms: D000293:Adolescent; D000328:Adult; D014408:Biomarkers, Tumor / Q000378:metabolism; D001859:Bone Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D009362:Neoplasm Metastasis; D012516:Osteosarcoma / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D057050:Receptor Tyrosine Kinase-like Orphan Receptors / Q000378:metabolism*; D012720:Severity of Illness Index; D051153:Wnt Proteins / Q000378:metabolism*; D000071818:Wnt-5a Protein; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D011518:Proto-Oncogene Proteins; C085389:WNT5A protein, human; D051153:Wnt Proteins; D000071818:Wnt-5a Protein; C078717:ROR2 protein, human; D057050:Receptor Tyrosine Kinase-like Orphan Receptors