title: Insulin-like growth factor 1 can promote the osteogenic differentiation and osteogenesis of stem cells from apical papilla.
abstract: Insulin-like growth factor 1 (IGF-1) plays an important role in the regulation of tooth root development, and stem cells from apical papilla (SCAPs) are responsible for the formation of root pulp and dentin. To date, it remains unclear whether IGF-1 can regulate the function of SCAPs. In this study, SCAPs were isolated and purified from human immature root apex, and stimulated by 100 ng/mL exogenous IGF-1. The effects of IGF-1 on the proliferation and differentiation of SCAPs were subsequently investigated. IGF-1 treated SCAPs presented the morphological and ultrastructural changes. Cell proliferation, alkaline phosphatase (ALP) activity and mineralization capacity of SCAPs were increased by IGF-1. Western blot and quantitative RT-PCR analyses further demonstrated that the expression of osteogenic-related proteins and genes (e.g., alkaline phosphatase, runt-related transcription factor 2, osterix, and osteocalcin) was significantly up-regulated in IGF-1 treated SCAPs, whereas the expression of odontoblast-specific markers (e.g., dentin sialoprotein and dentin sialophosphoprotein) was down-regulated by IGF-1. In vivo results revealed that IGF-1 treated SCAPs mostly gave birth to bone-like tissues while untreated SCAPs mainly generated dentin-pulp complex-like structures after transplantation. The present study revealed that IGF-1 can promote the osteogenic differentiation and osteogenesis capacity of SCAPs, but weaken their odontogenic differentiation and dentinogenesis capability, indicating that IGF-1 treated SCAPs can be used as a potential candidate for bone tissue engineering.
authors.affiliation: Institute of Stomatology, Nanjing Medical University, Nanjing, Jiangsu 210029, China.
authors.forename: Sainan
authors.initials: S
authors.lastname: Wang
authors.forename: Jinquan
authors.initials: J
authors.lastname: Mu
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Fan
authors.forename: Yan
authors.initials: Y
authors.lastname: Yu
authors.forename: Ming
authors.initials: M
authors.lastname: Yan
authors.forename: Gang
authors.initials: G
authors.lastname: Lei
authors.forename: Chunbo
authors.initials: C
authors.lastname: Tang
authors.forename: Zilu
authors.initials: Z
authors.lastname: Wang
authors.forename: Yangyu
authors.initials: Y
authors.lastname: Zheng
authors.forename: Jinhua
authors.initials: J
authors.lastname: Yu
authors.forename: Guangdong
authors.initials: G
authors.lastname: Zhang
mesh_terms: D000293:Adolescent; D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism; D003771:Dental Papilla / Q000166:cytology* / Q000473:pathology; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects*; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D012795:Sialoglycoproteins / Q000235:genetics / Q000378:metabolism; D000076182:Sp7 Transcription Factor; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D055815:Young Adult; D000099311:Dentin Sialophosphoprotein
chemical_list: D000469:Alkaline Phosphatase; D050659:Core Binding Factor Alpha 1 Subunit; D016326:Extracellular Matrix Proteins; D007334:Insulin-Like Growth Factor I; D015675:Osteocalcin; D010750:Phosphoproteins; D012795:Sialoglycoproteins; D000076182:Sp7 Transcription Factor; D014157:Transcription Factors; D000099311:Dentin Sialophosphoprotein; C493709:RUNX2 protein, human; C478163:SP7 protein, human