title: Nuclear microsatellite loci for Arabidopsis halleri (Brassicaceae), a model species to study plant adaptation to heavy metals.
abstract: OBJECTIVE
  Arabidopsis halleri is a model species to study the adaptation of plants to soils contaminated by zinc, cadmium, and lead. To provide a neutral genetic background with which adaptive genetic markers could be compared, we developed highly polymorphic neutral microsatellite markers.
  
  
  RESULTS
  Using a microsatellite-enriched library method, we identified 120 microsatellite loci for quantitative trait locus (QTL) mapping analysis, of which eight primer pairs were developed in a single multiplex for population genetic studies. Analyses were performed on 508 individuals from 26 populations. All loci were polymorphic with six to 23 alleles per locus. Genetic diversity varied between 0.56 and 0.76.
  
  
  CONCLUSIONS
  Our results demonstrated the value of these eight microsatellite markers to investigate neutral population genetic structure in A. halleri. To increase the resolution of population genetic analyses, we suggest adding them to the 11 markers previously developed independently.
authors.affiliation: Laboratoire de Génétique et Evolution des Populations Végétales, FRE CNRS 3268, Université des Sciences et Technologies de Lille, Lille1, F-59655 Villeneuve d'Ascq Cedex, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Godé
authors.forename: Isabelle
authors.initials: I
authors.lastname: Decombeix
authors.forename: Alicja
authors.initials: A
authors.lastname: Kostecka
authors.forename: Pawel
authors.initials: P
authors.lastname: Wasowicz
authors.forename: Maxime
authors.initials: M
authors.lastname: Pauwels
authors.forename: Adeline
authors.initials: A
authors.lastname: Courseaux
authors.forename: Pierre
authors.initials: P
authors.lastname: Saumitou-Laprade
mesh_terms: D000220:Adaptation, Biological; D000483:Alleles; D017360:Arabidopsis / Q000737:chemistry / Q000235:genetics*; D002104:Cadmium / Q000737:chemistry; D002467:Cell Nucleus / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D014644:Genetic Variation; D006579:Heterozygote; D007854:Lead / Q000737:chemistry; D018895:Microsatellite Repeats; D040641:Quantitative Trait Loci; D012989:Soil Pollutants / Q000737:chemistry; D013045:Species Specificity; D015032:Zinc / Q000737:chemistry
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D012989:Soil Pollutants; D002104:Cadmium; D007854:Lead; D015032:Zinc