title: Histamine via the histamine H₂-receptor reduces α-CD3-induced interferon-γ synthesis in murine CD4+ T cells in an indirect manner.
abstract: Histamine is involved in the execution of an immune reaction. Receptors for histamine, of which four different subtypes are known so far, are found on dendritic cells and on T cells. Via these receptors, histamine either indirectly or directly affects the activation of T cells. Data in the literature regarding the involved receptor subtypes and the mode of action of histamine on T cells are somewhat contradictory and depend on the type of cells analyzed, polarized T cells, or freshly prepared T cells within the context of the whole splenocyte population. Therefore, we analyzed the effect of histamine on murine T cells within splenocytes in a detailed manner. We stimulated freshly prepared splenocytes in the presence or absence of histamine with α-CD3 in vitro and analyzed the induced cytokine production. We show that histamine reduced the α-CD3-induced interferon-γ (IFN-γ) production of CD4⁺ cells via the histamine H₂-receptor. Moreover, the effect of histamine on the α-CD3-induced IFN-γ production could be transferred within conditioned splenocyte supernatants induced by histamine (in the absence of α-CD3). Thus, the histamine effect is mediated by a soluble factor, which, however, is neither of the classical anti-inflammatory mediators, interleukin-10, or transforming growth factor-β.
authors.affiliation: Institute of Pharmacology, Hannover Medical School, Hannover, Germany.
authors.forename: Marcus
authors.initials: M
authors.lastname: Vauth
authors.forename: Desirée
authors.initials: D
authors.lastname: Möhner
authors.forename: Silke
authors.initials: S
authors.lastname: Beermann
authors.forename: Roland
authors.initials: R
authors.lastname: Seifert
authors.forename: Detlef
authors.initials: D
authors.lastname: Neumann
mesh_terms: D000818:Animals; D017252:CD3 Complex / Q000378:metabolism*; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism*; D017077:Culture Media, Conditioned / Q000494:pharmacology; D005260:Female; D006632:Histamine / Q000494:pharmacology*; D007371:Interferon-gamma / Q000096:biosynthesis*; D016753:Interleukin-10 / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D011970:Receptors, Histamine H2 / Q000378:metabolism*; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000378:metabolism
chemical_list: D017252:CD3 Complex; D017077:Culture Media, Conditioned; D011970:Receptors, Histamine H2; D016212:Transforming Growth Factor beta; D016753:Interleukin-10; D006632:Histamine; D007371:Interferon-gamma