title: Genetic variation of IL-12B (+1188 region) is associated with its decreased circulating levels and susceptibility to Type 2 diabetes.
abstract: BACKGROUND
  Type 2 diabetes mellitus is one of the most common types of endocrine disease and the immune system plays a predominant role in its pathogenesis.
  
  
  OBJECTIVE
  The present study aimed to examine known gene polymorphisms within IL-12B (+1188) region and its circulating serum levels in Type 2 diabetic patients from the southeastern region of Iran and compare them with unrelated controls.
  
  
  METHODS
  In this clinical study, peripheral blood was collected from 114 Type 2 diabetic patients and 100 healthy controls. Serum levels of IL-12B were measured by ELISA. Genomic DNA was extracted from peripheral blood samples and polymorphisms at the +1188 position of the IL-12B gene were assessed using PCR restriction fragment-length polymorphism.
  
  
  RESULTS
  Our findings demonstrated that the AA genotype and the A allele of IL-12B were increased significantly in Type 2 diabetic patients when compared with controls. Our results also showed that the circulating levels of IL-12B were significantly decreased in Type 2 diabetic patients when compared with controls.
  
  
  CONCLUSIONS
  According to the findings of the current study, we concluded that IL-12B and its +1188 polymorphism may play a prominent role in the pathogenesis of Type 2 diabetes. Further replicative investigations using a larger sample size are essential to identify additional IL-12B genetic variants associated with a risk of Type 2 diabetes.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Rafsanjan University of Medical Sciences, Iran.
authors.forename: Narges
authors.initials: N
authors.lastname: Yaghini
authors.forename: Mehdi
authors.initials: M
authors.lastname: Mahmoodi
authors.forename: Gholamhossein
authors.initials: G
authors.lastname: Hassanshahi
authors.forename: Gholamreza
authors.initials: G
authors.lastname: Asadikaram
authors.forename: Mohammad Kazemi
authors.initials: MK
authors.lastname: Arababadi
authors.forename: Mohsen
authors.initials: M
authors.lastname: Rezaeian
authors.forename: Seyed Mohammad Ali
authors.initials: SM
authors.lastname: Sajjadi
authors.forename: Derek
authors.initials: D
authors.lastname: Kennedy
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D006801:Humans; D053762:Interleukin-12 Subunit p40 / Q000097:blood* / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic
chemical_list: D053762:Interleukin-12 Subunit p40