title: Inhibitory effects of Ginsenoside Rb1 on apoptosis caused by HSV-1 in human glioma cells.
abstract: To investigate the inhibitory effects of Ginsenoside Rb1 (GRb1) on apoptosis caused by Herpes Simplex Virus-1(HSV-1) in Human Glioma Cells (U251), U251 cells were infected by HSV-1 at a multiplicity of infection of 5 and GRb1, GRb1+HSV-1, HSV-1 and control groups. MTT and cell apoptosis assays were used to detect the inhibitory effects of GRb1 on the apoptosis of U251 cells that caused by HSV-1 infection for various concentrations of drug and virus treatments by MTT assay. We found that in the 400 μg/mL GRb1 and 400 μg/mL GRb1+HSV-1 groups, MTT values were higher than control group at all times (P<0. 05). Moreover, the apoptosis rate in the 400 μg/mL GRb1+HSV-1 group was lower than the HSV-1 group (P<0. 05). These results confirmed that, at appropriate concentrations, GRb1 could inhibit nerve cell apoptosis in HSV-1 infections.
authors.affiliation: Department of Microbiology, Key Laboratory of Medicine and Biotechnology of Qingdao, Qingdao University, Qingdao 266071, Shandong, China.
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Liang
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Dong-Meng
authors.initials: DM
authors.lastname: Qian
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.forename: Zhi-Hao
authors.initials: ZH
authors.lastname: Wang
authors.forename: Ming
authors.initials: M
authors.lastname: Hu
authors.forename: Xu-Xia
authors.initials: XX
authors.lastname: Song
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D036145:Ginsenosides / Q000378:metabolism*; D018259:Herpesvirus 1, Human / Q000472:pathogenicity*; D006801:Humans; D009457:Neuroglia / Q000187:drug effects* / Q000502:physiology*; D013194:Staining and Labeling / Q000379:methods; D013778:Tetrazolium Salts / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism
chemical_list: D036145:Ginsenosides; D013778:Tetrazolium Salts; D013844:Thiazoles; C442759:ginsenoside Rb1; C022616:thiazolyl blue