title: Neuregulin1 administration increases axonal elongation in dissociated primary sensory neuron cultures.
abstract: Neuregulin1 is a family of growth and differentiation factors involved in various functions of both peripheral and central nervous system including the regenerative processes that underlie regeneration of damaged peripheral nerves. In the present study we tested in vitro the effect of Neuregulin1 administration on dissociated rat dorsal root ganglion (DRG). Activity of neuregulin1 was compared to the activity of nerve growth factor in the same in vitro experimental model. Results showed that neurite outgrowth is enhanced by the addition of both neuregulin1 and nerve growth factor to the culture medium. While neuregulin1 was responsible for the growth of longer neurites, DRG neurons incubated with nerve growth factor showed shorter and more branched axons. Using enzyme-linked immunosorbent assay we also showed that the release of nerve growth factor, but not of brain derived neurotrophic factor is improved in DRG neuron treated with neuregulin1. On the other hand, the assay with growth factor blocking antibody, showed that effects exerted by neuregulin1 on neurite outgrowth is only partially due to the release of nerve growth factor. Taken together the results of this study provide a better understanding on the role of neuregulin1 in sensory neurons.
authors.affiliation: Department of Animal and Human Biology, University of Turin, via Accademia Albertina 13, 10123 Torino, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Audisio
authors.forename: Cristina
authors.initials: C
authors.lastname: Mantovani
authors.forename: Stefania
authors.initials: S
authors.lastname: Raimondo
authors.forename: Stefano
authors.initials: S
authors.lastname: Geuna
authors.forename: Isabelle
authors.initials: I
authors.lastname: Perroteau
authors.forename: Giorgio
authors.initials: G
authors.lastname: Terenghi
mesh_terms: D000818:Animals; D001369:Axons; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism; D002478:Cells, Cultured; D005727:Ganglia, Spinal / Q000166:cytology*; D008297:Male; D020932:Nerve Growth Factor / Q000378:metabolism / Q000494:pharmacology / Q000502:physiology; D020890:Neuregulin-1 / Q000494:pharmacology* / Q000502:physiology; D016501:Neurites / Q000378:metabolism; D061251:Primary Cell Culture; D051381:Rats; D017207:Rats, Sprague-Dawley; D011984:Sensory Receptor Cells / Q000166:cytology* / Q000187:drug effects; D014404:Tubulin / Q000378:metabolism
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D020890:Neuregulin-1; C490580:Nrg1 protein, rat; D014404:Tubulin; C117467:neuregulin beta; D020932:Nerve Growth Factor