title: Antagonistic activity of Lactobacillus acidophilus ATCC 4356 on the growth and adhesion/invasion characteristics of human Campylobacter jejuni.
abstract: The aim of this research was to determine the potential probiotic activity of Lactobacillus                         acidophilus ATCC 4356 against several human Campylobacter jejuni isolates. The ability to inhibit the pathogen's growth was evaluated by co-culture experiments as well as by antimicrobial assays with cell-free culture supernatant (CFCS), while interference with adhesion/invasion to intestinal Caco-2 cells was studied by exclusion, competition, and displacement tests. In the co-culture experiments L. acidophilus ATCC 4356 strain reduced the growth of C. jejuni with variable percentages of inhibition related to the contact time. The CFCS showed inhibitory activity against C. jejuni strains, stability to low pH, and thermal treatment and sensitivity to proteinase K and trypsin. L. acidophilus ATCC 4356 was able to reduce the adhesion and invasion to Caco-2 cells by most of the human C. jejuni strains. Displacement and exclusion mechanisms seem to be the preferred modalities, which caused a significant reduction of adhesion/invasion of pathogens to intestinal cells. The observed inhibitory properties of L. acidophilus ATCC 4356 on growth ability and on cells adhesion/invasion of C. jejuni may offer potential use of this strain for the management of Campylobacter infections.
authors.affiliation: Department of Biomolecular Science, Division of Toxicology, Hygienic and Environmental Science, University of Urbino Carlo Bo, via S. Chiara 27, Urbino, Italy.
authors.forename: Raffaella
authors.initials: R
authors.lastname: Campana
authors.forename: Sara
authors.initials: S
authors.lastname: Federici
authors.forename: Eleonora
authors.initials: E
authors.lastname: Ciandrini
authors.forename: Wally
authors.initials: W
authors.lastname: Baffone
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology; D000898:Antibiosis; D001422:Bacterial Adhesion; D018938:Caco-2 Cells; D016123:Campylobacter jejuni / Q000254:growth & development / Q000502:physiology*; D003470:Culture Media / Q000737:chemistry; D019286:Endopeptidase K / Q000378:metabolism; D004847:Epithelial Cells / Q000382:microbiology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007779:Lactobacillus acidophilus / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D050296:Microbial Viability / Q000187:drug effects / Q000528:radiation effects; D013696:Temperature; D014357:Trypsin / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D014357:Trypsin; D019286:Endopeptidase K