title: Removal of chlorophenolic derivatives by soil isolated ascomycete of Paraconiothyrium variabile and studying the role of its extracellular laccase.
abstract: The ability of Paraconiothyrium variabile, a laccase producing ascomycete recently isolated from soil, was studied to eliminate chlorophenol derivatives in submerged culture medium. Among the tested compounds, ρ-chlorophenol (ρ-CP) and pentachlorophenol (PCP) were found to have minimum and maximum toxic effects, respectively, on the growth of the microorganism and at the same time high and low bioelimination percentages. The fungal strain was able to remove 86% of ρ-CP (with initial concentration of 40 mg l(-1)) and 56% of 2,4-dichlorophenol (2,4-DCP; with same concentration as ρ-CP) after 9 days of incubation while no elimination was observed in the presence of 2,4,6-trichlorophenol (2,4,6-TCP) and PCP. Monitoring of laccase production level in the fermentation broth together with pollutant removal confirmed the key role of this copper-containing oxidase in chlorophenol derivatives elimination. The type of laccase inducer (guaiacol) and its final concentration (250 μM) and also initial pH of the fermentation broth (pH=5.5) in the elimination of ρ-CP increased the final removal yield from 86% to 94.3%.
authors.affiliation: Department of Pharmaceutical Biotechnology, Faculty of Pharmacy and Biotechnology Research Center, Tehran University of Medical Sciences, P.O. Box 14155-6451, Tehran 14174, Iran.
authors.forename: Hamid
authors.initials: H
authors.lastname: Forootanfar
authors.forename: Mohammad Mehdi
authors.initials: MM
authors.lastname: Movahednia
authors.forename: Soheila
authors.initials: S
authors.lastname: Yaghmaei
authors.forename: Minoosadat
authors.initials: M
authors.lastname: Tabatabaei-Sameni
authors.forename: Hossein
authors.initials: H
authors.lastname: Rastegar
authors.forename: Armin
authors.initials: A
authors.lastname: Sadighi
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Faramarzi
mesh_terms: D001203:Ascomycota / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002733:Chlorophenols / Q000302:isolation & purification* / Q000378:metabolism; D003470:Culture Media; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D042845:Laccase / Q000378:metabolism*; D012988:Soil Microbiology
chemical_list: D002733:Chlorophenols; D003470:Culture Media; D042845:Laccase