title: A label-free thrombin binding aptamer as a probe for highly sensitive and selective detection of lead(II) ions by a resonance Rayleigh scattering method.
abstract: The binding of lead(II) ions with unusually high affinity to a thrombin binding aptamer resulted in an enhancement of resonance Rayleigh scattering (RRS). A simple, sensitive, and selective assay for the direct determination of trace amounts of Pb(2+) on the basis of RRS has been proposed.
authors.affiliation: Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, P. R. China.
authors.forename: Xiao Bei
authors.initials: XB
authors.lastname: Fu
authors.forename: Fei
authors.initials: F
authors.lastname: Qu
authors.forename: Nian Bing
authors.initials: NB
authors.lastname: Li
authors.forename: Hong Qun
authors.initials: HQ
authors.lastname: Luo
mesh_terms: D052157:Aptamers, Nucleotide / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D004785:Environmental Pollutants / Q000032:analysis; D007854:Lead / Q000032:analysis*; D008027:Light; D015345:Oligonucleotide Probes / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D012542:Scattering, Radiation; D013917:Thrombin / Q000378:metabolism*; D014867:Water / Q000737:chemistry
chemical_list: D052157:Aptamers, Nucleotide; D004785:Environmental Pollutants; D015345:Oligonucleotide Probes; D014867:Water; D007854:Lead; D013917:Thrombin