title: A luminescence study of the interaction of sulfobutylether-β-cyclodextrin with rutin.
abstract: The luminol/sulfobutylether-β-cyclodextrin (SBE(7M)-β-CD) chemiluminescence (CL) system and the interaction of SBE(7M)-β-CD/rutin were first described by flow injection (FI) CL method. It was found that SBE(7M)-β-CD with luminol could form 1:1 complex online, which could accelerate the electrons transferring rate of excited 3-aminophthalate, giving the enhanced CL intensity of luminol. The enhancement of CL intensity was proportional to the concentrations of SBE(7M)- β-CD with a linear range from 25 to 1750 μmol L⁻¹. It was also found that rutin could inhibit the CL intensity from luminol/SBE(7M)-β-CD system, and the decrement of CL intensity was logarithm over the concentrations of rutin ranging from 0.1 to 100.0 nmol L⁻¹, giving the regression equation ΔI = 32.90lgC(rutin) + 16.26 (R² = 0.9952) with a detection limit of 0.03 nmol L⁻¹ (3σ). According to the proposed CL model, the binding constant (K(CD-R)) and the stoichiometric ratio of SBE(7M)-β-CD/rutin complex were obtained as 1.6 × 10⁶ L² mol⁻² and 2:1. The possible mechanism of luminol/SBE(7M)-β- CD/rutin interaction was also discussed. The method was successfully applied to monitor rutin in human urine samples after ingesting SBE(7M)-β-CD/rutin complex, with a total excretion of 68.8% within 8.0 h.
authors.affiliation: Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry & Material Science, Northwest University, Xi'an, 710069, China.
authors.forename: Min
authors.initials: M
authors.lastname: Wu
authors.forename: Zhenghua
authors.initials: Z
authors.lastname: Song
authors.forename: Jingjing
authors.initials: J
authors.lastname: Zhang
mesh_terms: D002138:Calibration; D002626:Chemistry, Pharmaceutical; D004339:Drug Compounding; D005079:Excipients / Q000737:chemistry*; D017022:Flow Injection Analysis / Q000592:standards; D006801:Humans; D057230:Limit of Detection; D049408:Luminescent Agents / Q000737:chemistry*; D008165:Luminol / Q000737:chemistry*; D012015:Reference Standards; D012044:Regression Analysis; D015203:Reproducibility of Results; D012431:Rutin / Q000032:analysis / Q000097:blood / Q000737:chemistry* / Q000493:pharmacokinetics / Q000652:urine; D013050:Spectrometry, Fluorescence / Q000592:standards; D013607:Tablets; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D005079:Excipients; D049408:Luminescent Agents; D013607:Tablets; D047392:beta-Cyclodextrins; C093196:SBE4-beta-cyclodextrin; D008165:Luminol; D012431:Rutin