title: Novel strategy for selection of monoclonal antibodies against highly conserved antigens: phage library panning against ephrin-B2 displayed on yeast.
abstract: Ephrin-B2 is predominately expressed in endothelium of arterial origin, involved in developmental angiogenesis and neovasculature formation through its interaction with EphB4. Despite its importance in physiology and pathological conditions, it has been challenging to produce monoclonal antibodies against ephrin-B2 due to its high conservation in sequence throughout human and rodents. Using a novel approach for antibody selection by panning a phage library of human antibody against antigens displayed in yeast, we have isolated high affinity antibodies against ephrin-B2. The function of one high affinity binder (named as 'EC8') was manifested in its ability to inhibit ephrin-B2 interaction with EphB4, to cross-react with murine ephrin-B2, and to induce internalization into ephrin-B2 expressing cells. EC8 was also compatible with immunoprecipitation and detection of ephrin-B2 expression in the tissue after standard chemical fixation procedure. Consistent with previous reports on ephrin-B2 induction in some epithelial tumors and tumor-associated vasculatures, EC8 specifically detected ephrin-B2 in tumors as well as the vasculature within and outside of the tumors. We envision that monoclonal antibody developed in this study may be used as a reagent to probe ephrin-B2 distribution in normal as well as in pathological conditions and to antagonize ephrin-B2 interaction with EphB4 for basic science and therapeutic applications.
authors.affiliation: Department of Biomedical Engineering, Cornell University, Ithaca, New York, United States of America.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Gu
authors.forename: Yogindra
authors.initials: Y
authors.lastname: Vedvyas
authors.forename: Xiaoyue
authors.initials: X
authors.lastname: Chen
authors.forename: Tanwi
authors.initials: T
authors.lastname: Kaushik
authors.forename: Chang-Il
authors.initials: CI
authors.lastname: Hwang
authors.forename: Xuebo
authors.initials: X
authors.lastname: Hu
authors.forename: Alexander Y
authors.initials: AY
authors.lastname: Nikitin
authors.forename: Moonsoo M
authors.initials: MM
authors.lastname: Jin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification* / Q000378:metabolism / Q000627:therapeutic use; D000941:Antigens / Q000737:chemistry / Q000276:immunology*; D016466:CHO Cells; D002478:Cells, Cultured; D017124:Conserved Sequence / Q000276:immunology*; D006224:Cricetinae; D003412:Cricetulus; D036388:Ephrin-B2 / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D045325:HCT116 Cells; D057809:HEK293 Cells; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D008954:Models, Biological; D008969:Molecular Sequence Data; D019151:Peptide Library; D011485:Protein Binding; D036224:Receptor, EphB4 / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D023041:Xenograft Model Antitumor Assays; D015003:Yeasts / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens; D036388:Ephrin-B2; D019151:Peptide Library; D036224:Receptor, EphB4