title: Severity of Escherichia coli bacteraemia is independent of the intrinsic virulence of the strains assessed in a mouse model.
abstract: Extraintestinal pathogenic Escherichia coli (ExPEC) strains, a major cause of bacteraemia, typically belong to phylogenetic group B2 and express diverse accessory traits that contribute to virulence in mouse infection models. However, their high genomic diversity obscures the relationship between virulence factors and severity of infection in patients. In this study, we analysed concomitantly the strain's expression of virulence in a mouse model, genomic determinants and the clinical severity of infection in 60 bacteraemic patients. We show that bacterial virulence based on an animal model study and virulence factor determination is not correlated with pejorative outcome of E. coli human blood infections.
authors.affiliation: INSERM, U895, Centre Méditerranéen de Médecine Moléculaire, C3M, Microbial Toxins and Host Interactions; University Nice-Sophia-Antipolis, IFR50, UFR Médecine; Laboratoire de Bactériologie, Centre Hospitalier Universitaire de Nice, Hôpital Archet II, CHU Nice. Electronic address: xavier.nassif@inserm.fr.
authors.forename: L
authors.initials: L
authors.lastname: Landraud
authors.affiliation: INSERM, UMR-S 722; University Paris Nord, Sorbonne Paris Cité, UFR Santé, Médecine, Biologie Humaine; Laboratoire de Bactériologie, Assistance Publique Hôpitaux de Paris, AP-HP, Hôpital Avicenne, Bobigny.
authors.forename: F
authors.initials: F
authors.lastname: Jauréguy
authors.affiliation: INSERM, U1002.
authors.forename: E
authors.initials: E
authors.lastname: Frapy
authors.affiliation: Institut Pasteur, Genotyping of Pathogens and Public Health, Paris.
authors.forename: G
authors.initials: G
authors.lastname: Guigon
authors.affiliation: Laboratoire de Bacteriologie, CHU de Brest, University Bretagne Occidentale, Brest.
authors.forename: S
authors.initials: S
authors.lastname: Gouriou
authors.affiliation: Faculté de Médecine, Hôpital Européen Georges Pompidou, AP-HP, and Paris Descartes.
authors.forename: E
authors.initials: E
authors.lastname: Carbonnelle
authors.affiliation: INSERM, UMR-S 722; Sorbonne Paris Cité, Faculté de Médecine, University Paris Diderot, Site Xavier Bichat.
authors.forename: O
authors.initials: O
authors.lastname: Clermont
authors.affiliation: INSERM, UMR-S 722; Sorbonne Paris Cité, Faculté de Médecine, University Paris Diderot, Site Xavier Bichat.
authors.forename: E
authors.initials: E
authors.lastname: Denamur
authors.affiliation: INSERM, UMR-S 722; University Paris Nord, Sorbonne Paris Cité, UFR Santé, Médecine, Biologie Humaine; Laboratoire de Bactériologie, Assistance Publique Hôpitaux de Paris, AP-HP, Hôpital Avicenne, Bobigny.
authors.forename: B
authors.initials: B
authors.lastname: Picard
authors.affiliation: INSERM, U895, Centre Méditerranéen de Médecine Moléculaire, C3M, Microbial Toxins and Host Interactions; University Nice-Sophia-Antipolis, IFR50, UFR Médecine.
authors.forename: E
authors.initials: E
authors.lastname: Lemichez
authors.affiliation: Institut Pasteur, Genotyping of Pathogens and Public Health, Paris.
authors.forename: S
authors.initials: S
authors.lastname: Brisse
authors.affiliation: INSERM, U1002; Faculté de Médecine, University Paris Descartes; Laboratoire de Bactériologie, AP-HP, Hôpital Necker-Enfants Malades, Paris, France.
authors.forename: X
authors.initials: X
authors.lastname: Nassif
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D016470:Bacteremia / Q000382:microbiology*; D016009:Chi-Square Distribution; D004195:Disease Models, Animal; D004926:Escherichia coli / Q000235:genetics / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000382:microbiology*; D005260:Female; D020869:Gene Expression Profiling; D005798:Genes, Bacterial; D006801:Humans; D051379:Mice; D015233:Models, Statistical; D010802:Phylogeny; D014774:Virulence