title: Wide-range protein photo-crosslinking achieved by a genetically encoded N(ε)-(benzyloxycarbonyl)lysine derivative with a diazirinyl moiety.
abstract: A derivative of N(ε)-benzyloxycarbonyl-L-lysine with a photo-reactive diazirinyl group, N(ε)-[((4-(3-(trifluoromethyl)-3H-diazirin-3-yl)benzyl)oxy)carbonyl]-L-lysine, was site-specifically incorporated into target proteins in mammalian cells. The incorporated photo-crosslinker is able to react not only with residues as distant as about 15 Å but also with those in closer proximity, thus enabling "wide-range" photo-crosslinking of proteins.
authors.affiliation: RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi, Yokohama 230-0045, Japan.
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Yanagisawa
authors.forename: Nobumasa
authors.initials: N
authors.lastname: Hino
authors.forename: Fumie
authors.initials: F
authors.lastname: Iraha
authors.forename: Takahito
authors.initials: T
authors.lastname: Mukai
authors.forename: Kensaku
authors.initials: K
authors.lastname: Sakamoto
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Yokoyama
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001389:Azirines / Q000737:chemistry*; D001577:Benzophenones / Q000737:chemistry; D016466:CHO Cells; D006224:Cricetinae; D003432:Cross-Linking Reagents; D057809:HEK293 Cells; D006801:Humans; D008239:Lysine / Q000031:analogs & derivatives* / Q000737:chemistry; D010649:Phenylalanine / Q000031:analogs & derivatives* / Q000737:chemistry; D011487:Protein Conformation
chemical_list: C488060:4-benzoylphenylalanine; D048868:Adaptor Proteins, Signal Transducing; D001389:Azirines; D001577:Benzophenones; D003432:Cross-Linking Reagents; C573114:N(epsilon)-(benzyloxycarbonyl)lysine; D010649:Phenylalanine; C044966:3-(4-(3-(trifluoromethyl)-3H-diazirin-3-yl)phenyl)alanine; D008239:Lysine