title: Glutathione peroxidase activity in seminal plasma and its relationship to classical sperm parameters and in vitro fertilization-intracytoplasmic sperm injection outcome.
abstract: OBJECTIVE
  To relate the glutathione peroxidase (GPX) activity level in human seminal plasma with standard semen parameters and spermatozoa fertilization potential in terms of fertilization and pregnancy rates in an IVF program.
  
  
  METHODS
  Prospective study.
  
  
  METHODS
  Human Reproduction Unit at Cruces Hospital (Vizcaya, Spain).
  
  
  METHODS
  Three hundred consecutive males from infertile couples participating in the IVF program.
  
  
  METHODS
  None.
  
  
  METHODS
  Analysis of GPX activity in seminal plasma by spectrophotometry.
  
  
  RESULTS
  GPX activity in seminal plasma was significantly lower in patients with abnormal sperm as assessed by 1999 and 2010 World Health Organization (WHO) criteria, compared with normozoospermic individuals. There was a more significant decrease in those samples with severe sperm pathologies. GPX values were significantly lower in samples with severe asthenozoospermia, oligozoospermia, and teratozoospermia compared with normal samples. However, there was no correlation between GPX activity in seminal plasma in IVF patients and fertilization rates or pregnancy outcome.
  
  
  CONCLUSIONS
  Although seminal plasma GPX activity was related to semen quality according to WHO parameters, such an association was not found with IVF-intracytoplasmic sperm injection (ICSI) outcome, presumably because of the well-known ability of IVF-ICSI procedures to overcome sperm deficiencies in the fertilization process.
authors.affiliation: Human Reproduction Unit, Department of Obstetrics and Gynecology, Hospital de Cruces, University of Basque Country, Vizcaya, Spain. lorena.crisolortiz@osakidetza.net
authors.forename: Lorena
authors.initials: L
authors.lastname: Crisol
authors.forename: Roberto
authors.initials: R
authors.lastname: Matorras
authors.forename: Fermín
authors.initials: F
authors.lastname: Aspichueta
authors.forename: Antonia
authors.initials: A
authors.lastname: Expósito
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Hernández
authors.forename: Maria Begoña
authors.initials: MB
authors.lastname: Ruiz-Larrea
authors.forename: Rosario
authors.initials: R
authors.lastname: Mendoza
authors.forename: José Ignacio
authors.initials: JI
authors.lastname: Ruiz-Sanz
mesh_terms: D000328:Adult; D053627:Asthenozoospermia / Q000201:enzymology / Q000473:pathology / Q000628:therapy*; D015415:Biomarkers / Q000032:analysis; D048430:Cell Shape; D005260:Female; D005307:Fertilization in Vitro; D005979:Glutathione Peroxidase / Q000032:analysis*; D006801:Humans; D008297:Male; D009845:Oligospermia / Q000201:enzymology / Q000473:pathology / Q000628:therapy*; D011237:Predictive Value of Tests; D011247:Pregnancy; D018873:Pregnancy Rate; D012661:Semen / Q000201:enzymology*; D012720:Severity of Illness Index; D013056:Spectrophotometry, Ultraviolet; D013076:Sperm Count; D020554:Sperm Injections, Intracytoplasmic; D013081:Sperm Motility; D013094:Spermatozoa / Q000473:pathology*; D016896:Treatment Outcome
chemical_list: D015415:Biomarkers; D005979:Glutathione Peroxidase