title: Calpastatin upregulation in Mycoplasma hyorhinis-infected cells is promoted by the mycoplasma lipoproteins via the NF-κB pathway.
abstract: Mycoplasma hyorhinis frequently contaminates cultured cells, with effects on synthetic and metabolic pathways. We demonstrated for the first time that contamination of cells by a strain of M. hyorhinis (NDMh) results in increased levels of calpastatin (the endogenous inhibitor of the ubiquitous Ca(2+) -dependent protease calpain). We now show that the calpastatin upregulation by NDMh in neuroblastoma SH-SY5Y cells resides in the NDMh lipoprotein fraction (LPP), via the NF-κB transcription pathway. NF-κB activation requires dissociation of the cytoplasmic NF-κB/IκB complex followed by NF-κB translocation to the nucleus. NDMh-LPP induced translocation of the NF-κB RelA subunit to the nucleus and upregulated calpastatin. RelA translocation and calpastatin elevation were prevented when dissociation of the NF-κB/IκB complex was inhibited either by transfection with the non-phosphorylatable IκB mutant ΔNIκBα, or by using PS1145, an inhibitor of the IκB kinase (IKK complex). Increased calpastatin levels attenuate calpain-related amyloid-β-peptide and Ca(2+) -toxicity (these are central to the pathogenesis of Alzheimer's Disease). LPP-induced elevation of calpastatin provides an example of effects on non-inflammatory intracellular proteins, the outcome being significant alterations in host cell functions. Since calpastatin level is important in the control of calpain activity, mycoplasmal LPP may be of interest in treating some pathological processes involving excessive calpain activation.
authors.affiliation: Department of Human Molecular Genetics and Biochemistry, Sackler School of Medicine, Tel-Aviv University, Ramat-Aviv, Tel-Aviv 69978, Israel.
authors.forename: Esther
authors.initials: E
authors.lastname: Elkind
authors.forename: Tali
authors.initials: T
authors.lastname: Vaisid
authors.forename: Jonathan D
authors.initials: JD
authors.lastname: Kornspan
authors.forename: Sivia
authors.initials: S
authors.lastname: Barnoy
authors.forename: Shlomo
authors.initials: S
authors.lastname: Rottem
authors.forename: Nechama S
authors.initials: NS
authors.lastname: Kosower
mesh_terms: D016229:Amyloid beta-Peptides / Q000494:pharmacology / Q000502:physiology; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism / Q000502:physiology; D061207:Calcium Ionophores / Q000494:pharmacology; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D002154:Calpain / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism; D045744:Cell Line, Tumor; D054884:Host-Pathogen Interactions; D006801:Humans; D015759:Ionomycin / Q000494:pharmacology; D008074:Lipoproteins / Q000378:metabolism / Q000502:physiology; D008840:Microfilament Proteins / Q000378:metabolism; D009175:Mycoplasma Infections / Q000378:metabolism* / Q000382:microbiology; D045790:Mycoplasma hyorhinis / Q000378:metabolism / Q000502:physiology*; D016328:NF-kappa B / Q000378:metabolism*; D015398:Signal Transduction; D015854:Up-Regulation; D013049:Spectrin
chemical_list: D016229:Amyloid beta-Peptides; D001425:Bacterial Outer Membrane Proteins; D061207:Calcium Ionophores; D002135:Calcium-Binding Proteins; D002154:Calpain; D002352:Carrier Proteins; D015759:Ionomycin; D008074:Lipoproteins; D008840:Microfilament Proteins; D016328:NF-kappa B; D013049:Spectrin; C033668:calpastatin