title: Detection of 1311 polymorphism in the glucose-6-phosphate dehydrogenase gene by microarray technique.
abstract: BACKGROUND
  Nucleotide 1311 polymorphism at exon 11 of the glucose-6-phosphate dehydrogenase (G6PD) gene is fairly common in various populations worldwide, especially among Mediterranean populations. In this study, 1311 polymorphism in G6PD-deficient cases was identified by microarray technique.
  
  
  METHODS
  Four hundred and fifty clinically healthy subjects were screened and 32 cases were found to have G6PD deficiency (7.11%). Our analysis of genomic DNA samples from 32 G6PD-deficient individuals revealed that the number and percentage of subjects who had a C-to-T alteration at nucleotide 1311 were 21 and 4.7% respectively. Given that the frequency of 1311 polymorphism has been reported in previous studies to be fairly high among G6PD-deficient people with the Mediterranean mutation, our data seem to be inconsistent with what we would expect for this particular region.
  
  
  RESULTS
  The highly diverse ethnic background of the Adana population which probably results from the high level of immigration into this part of Turkey may be one of the most sensible explanations for this unexpected finding. Nevertheless, it seems that our results need to be confirmed in larger studies.
  
  
  CONCLUSIONS
  The polymorphism studies in the G6PD gene may help us to illuminate the genetic basis of the G6PD deficiency in different regions and in various ethnic groups, and also to discover the influence of a specific polymorphism on the clinical course of the deficiency.
authors.affiliation: Cukurova University, Vocational School of Health Services, Adana, Turkey.
authors.forename: Sule
authors.initials: S
authors.lastname: Menziletoglu Yildiz
authors.forename: Sedefgul
authors.initials: S
authors.lastname: Yuzbasioglu Ariyurek
authors.forename: Murat
authors.initials: M
authors.lastname: Tahiroglu
authors.forename: Kiymet
authors.initials: K
authors.lastname: Aksoy