title: RIC-7 promotes neuropeptide secretion.
abstract: Secretion of neurotransmitters and neuropeptides is mediated by exocytosis of distinct secretory organelles, synaptic vesicles (SVs) and dense core vesicles (DCVs) respectively. Relatively little is known about factors that differentially regulate SV and DCV secretion. Here we identify a novel protein RIC-7 that is required for neuropeptide secretion in Caenorhabditis elegans. The RIC-7 protein is expressed in all neurons and is localized to presynaptic terminals. Imaging, electrophysiology, and behavioral analysis of ric-7 mutants indicates that acetylcholine release occurs normally, while neuropeptide release is significantly decreased. These results suggest that RIC-7 promotes DCV-mediated secretion.
authors.affiliation: Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts, United States of America.
authors.forename: Yingsong
authors.initials: Y
authors.lastname: Hao
authors.forename: Zhitao
authors.initials: Z
authors.lastname: Hu
authors.forename: Derek
authors.initials: D
authors.lastname: Sieburth
authors.forename: Joshua M
authors.initials: JM
authors.lastname: Kaplan
mesh_terms: D000109:Acetylcholine / Q000378:metabolism; D000448:Aldicarb; D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism; D019852:Diacylglycerol Kinase / Q000235:genetics / Q000378:metabolism; D004342:Drug Hypersensitivity / Q000235:genetics; D005089:Exocytosis; D018482:Muscle, Skeletal / Q000187:drug effects; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D009479:Neuropeptides / Q000235:genetics* / Q000378:metabolism*; D017729:Presynaptic Terminals / Q000378:metabolism*; D022142:Secretory Vesicles / Q000235:genetics / Q000378:metabolism; D013572:Synaptic Vesicles / Q000235:genetics / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
chemical_list: D029742:Caenorhabditis elegans Proteins; D009419:Nerve Tissue Proteins; D009479:Neuropeptides; C572928:Ric-7 protein, C elegans; D005680:gamma-Aminobutyric Acid; D000448:Aldicarb; D019852:Diacylglycerol Kinase; D000109:Acetylcholine