title: [Effects of nicotine on bone marrow stromal cells proliferation and differentiation of chondrocyte in vitro].
abstract: OBJECTIVE
  To examine the effects of various concentration of nicotine on bone marrow stromal cells (BMSCs) proliferation and differentiation of cartilaginous in vitro.
  
  
  METHODS
  BMSCs was obtained from femoral bone and tibia of New-Zealand albino rabbit. The cells of the 3rd generation were used in study. Different concentration of nicotine (0, 1 x 10(-7), 1 x 10(-6), 1 x 10(-5) M) were added into BMSCs. BMSCs proliferation was analyzed by MTT assay at the 1, 4, 7, 14 days. The expression of collagen type II and aggrecan as the marker genes of cartilaginous differentiation from BMSCs were detected by reverse transcriptase-polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  Microscope showed that BMSCs transformed from round to fusiform shape. The concentration of nicotine in 1 x 10(-7), 1 x 10(-6) M had a significant positive effect on cell proliferation and the expression of type II collagen in a time-dependent manner when supplemented in commonly used induction media (P<0.05). Concentrations of nicotine in 1 x 10(-7) can promote the expression of aggrecan at the 7th day after induction,and in 1 x 10(-5) M may inhibit the expression of type II collagen and aggrecan.
  
  
  CONCLUSIONS
  It was implied that local application of nicotine at an appropriate concentration may be a promising approach for enhancing cartilaginous differentiation capacity of BMSCs in cartilage tissue engineering.
authors.affiliation: Department of Orthopaedics Surgery, the Second Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, Zhejiang, China.
authors.forename: Xiao-zhou
authors.initials: XZ
authors.lastname: Ying
authors.forename: Lei
authors.initials: L
authors.lastname: Peng
authors.forename: Shao-wen
authors.initials: SW
authors.lastname: Cheng
authors.forename: Qing-yu
authors.initials: QY
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Dong-quan
authors.initials: DQ
authors.lastname: Kou
authors.forename: Yue
authors.initials: Y
authors.lastname: Shen
mesh_terms: D053674:Aggrecans / Q000235:genetics; D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects*; D024043:Collagen Type II / Q000235:genetics; D008297:Male; D009538:Nicotine / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis; D011817:Rabbits; D017154:Stromal Cells / Q000187:drug effects / Q000502:physiology
chemical_list: D053674:Aggrecans; D024043:Collagen Type II; D012333:RNA, Messenger; D009538:Nicotine