title: Rhesus rotavirus trafficking during entry into MA104 cells is restricted to the early endosome compartment.
abstract: Endocytosis has recently been implicated in rotavirus (RV) entry. We examined the role of Rabs, which regulate endosomal trafficking, during RV entry. Several structural proteins of neuraminidase-sensitive and -insensitive RVs colocalized with Rab5, an early endosome marker, but not Rab7, a late endosome marker. Dominant-negative and constitutively active mutants demonstrated that Rab5 but not Rab4 or Rab7 affects rhesus RV (RRV) infectivity. These data suggest that early RRV trafficking is confined to the early endosome compartment and requires Rab5.
authors.affiliation: Departments of Medicine and Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Marie
authors.initials: M
authors.lastname: Wolf
authors.forename: Emily M
authors.initials: EM
authors.lastname: Deal
authors.forename: Harry B
authors.initials: HB
authors.lastname: Greenberg
mesh_terms: D000818:Animals; D002460:Cell Line; D004705:Endocytosis; D011992:Endosomes / Q000821:virology*; D008253:Macaca mulatta; D018419:Primate Diseases / Q000201:enzymology / Q000503:physiopathology / Q000821:virology*; D012401:Rotavirus / Q000235:genetics / Q000502:physiology*; D012400:Rotavirus Infections / Q000201:enzymology / Q000503:physiopathology / Q000662:veterinary* / Q000821:virology; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D053586:Virus Internalization; D020696:rab5 GTP-Binding Proteins / Q000378:metabolism
chemical_list: D014764:Viral Proteins; D020696:rab5 GTP-Binding Proteins