title: Silibinin regulates gene expression, production and secretion of mucin from cultured airway epithelial cells.
abstract: We investigated whether silibinin significantly affects gene expression, production and secretion of mucin from cultured airway epithelial cells. Confluent NCI-H292 cells were pretreated with silibinin for 30 min and then stimulated with epidermal growth factor (EGF), phorbol 12-myristate 13-acetate (PMA) or TNF-α for 24 h. The MUC5AC mucin gene expression and mucin protein production were measured by reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). The effect of silibinin on TNF-α-induced activation of NF-κB p65 was also examined. Confluent primary rat tracheal surface epithelial (RTSE) cells were pretreated with adenosine triphosphate (ATP) for 5 min and then treated for 30 min in the presence of silibinin to assess the effect on mucin secretion using ELISA. The results were as follows: (i) silibinin inhibited the expression of the MUC5AC mucin gene induced by EGF, PMA or TNF-α from NCI-H292 cells; (ii) silibinin also inhibited the production of MUC5AC mucin protein induced by the same inducers from NCI-H292 cells; (iii) silibinin inhibited the activation of NF-κB p65 by TNF-α in NCI-H292 cells; (iv) silibinin significantly decreased ATP-induced mucin secretion from cultured RTSE cells. This result suggests that silibinin can regulate gene expression, production and secretion of mucin by directly acting on airway epithelial cells.
authors.affiliation: Department of Thoracic Surgery, College of Medicine, Eulji University, Daejeon, Korea.
authors.forename: Kil-Dong
authors.initials: KD
authors.lastname: Kim
authors.forename: Hyun Jae
authors.initials: HJ
authors.lastname: Lee
authors.forename: Seung Pyong
authors.initials: SP
authors.lastname: Lim
authors.forename: Asaduzzaman
authors.initials: A
authors.lastname: Sikder
authors.forename: Su Yel
authors.initials: SY
authors.lastname: Lee
authors.forename: Choong Jae
authors.initials: CJ
authors.lastname: Lee
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004797:Enzyme-Linked Immunosorbent Assay; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D055271:Mucin 5AC / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000077385:Silybin; D012838:Silymarin / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D051996:Transcription Factor RelA / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D055271:Mucin 5AC; C498143:RELA protein, human; D012838:Silymarin; D051996:Transcription Factor RelA; D014409:Tumor Necrosis Factor-alpha; D000077385:Silybin; D004815:Epidermal Growth Factor; D013755:Tetradecanoylphorbol Acetate