title: [Apolipoprotein(a) isoforms immunoblotting detection: comparative study of two methods].
abstract: This study reports the comparison between two methods (chemiluminescence and enzymatic colorimetry) for revelation of apolipoprotein(a) [apo(a)] isoforms by immunoblotting in 102 Ivorian healthy subjects. Apo(a) isoform sizes were determined by sodium dodecyl sulfate-agarose-polyacrylamide gel electrophoresis (SDS-PAGE) followed by immunoblotting using enzymatic colorimetry or chemiluminescence. Within-run precision was comprised between 4.9% and 9.2% for colorimetry and between 2.9% and 4.6% for chemiluminescence. Both methods have detected apo(a) isoforms in all patients, even when lipoprotein(a) concentrations were under detection limit (0.02 g/L). The two methods were significantly correlated (r = 0.96 to 0.98, p<0.0001). Even though the chemiluminescence method exhibited better performances than the colorimetric method, both techniques could be used indifferently.
authors.affiliation: Laboratoire de biochimie, UFR sciences pharmaceutiques et biologiques, Université de Cocody, Abidjan, Côte d'Ivoire. angelake@aviso.ci
authors.forename: Angèle
authors.initials: A
authors.lastname: Edjème-Aké
authors.forename: Roselyne
authors.initials: R
authors.lastname: Garnotel
authors.forename: Sandrine
authors.initials: S
authors.lastname: Vallé-Polneau
authors.forename: Hugues
authors.initials: H
authors.lastname: Ahiboh
authors.forename: Marie Laure
authors.initials: ML
authors.lastname: Hauhouot-Attoungbré
authors.forename: Dagui
authors.initials: D
authors.lastname: Monnet
authors.forename: Philippe
authors.initials: P
authors.lastname: Gillery
mesh_terms: D000293:Adolescent; D000328:Adult; D053241:Apoprotein(a) / Q000032:analysis* / Q000097:blood / Q000378:metabolism*; D001782:Blood Donors; D003124:Colorimetry / Q000379:methods; D007560:Cote d'Ivoire; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D008163:Luminescent Measurements / Q000379:methods; D008875:Middle Aged; D008970:Molecular Weight; D020033:Protein Isoforms / Q000032:analysis / Q000378:metabolism; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: D020033:Protein Isoforms; D053241:Apoprotein(a)