title: The Ussing chamber and measurement of drug actions on mucosal ion transport.
abstract: Ussing chamber technology can be used to investigate the pharmacodynamic and pharmacokinetic properties of drugs and natural substances on polarized epithelia. With this approach, epithelial sheets or confluent epithelial cell monolayers are mounted between two half-chambers, permitting compound delivery to either the apical or basolateral cell surface. Described in this unit are protocols for determining the effects of test compounds on transepithelial ion transport processes.
authors.affiliation: University of Minnesota, Department of Veterinary & Biomedical Sciences, St. Paul, Minnesota, USA.
authors.forename: David R
authors.initials: DR
authors.lastname: Brown
authors.forename: Scott M
authors.initials: SM
authors.lastname: O'Grady
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D015194:Diffusion Chambers, Culture; D004594:Electrophysiology / Q000295:instrumentation*; D004867:Equipment Design; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000378:metabolism; D016623:Ion Pumps / Q000502:physiology; D017136:Ion Transport / Q000187:drug effects*; D008564:Membrane Potentials / Q000502:physiology; D010599:Pharmacokinetics; D011868:Radioisotopes; D051381:Rats; D013048:Specimen Handling / Q000379:methods; D014022:Tissue Survival
chemical_list: D016623:Ion Pumps; D011868:Radioisotopes