title: Development of RNA aptamer and its ligand binding assay on microchip electrophoresis.
abstract: Microchip electrophoresis (ME) coupled with fluorescence detection was used to estimate the binding activity of aptamer in each systematic evolution of ligands by exponential enrichment (SELEX) round for a target molecule. This approach is a non-radioisotopic, rapid and simple platform, and electrophoretic separation appears to be an effective technique for aptamers of oligonucleotide molecules. We tried to obtain gonadotropin-specific RNA aptamer by the above approach. As a result, the peaks of aptamers based on the conformational differences between them were separated and detected on the electropherograms. Moreover, the intensity of peak of unbound aptamer was decreased with progression through the SELEX rounds, suggesting that RNA aptamer with high affinity was obtained by the proposed method.
authors.affiliation: School of Pharmacy Showa University, 1-5-8 Hatanodai, Shinagawa, Tokyo 142-8555, Japan.
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Ohno
authors.forename: Chikara
authors.initials: C
authors.lastname: Nakata
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Sano
authors.forename: Fumiko
authors.initials: F
authors.lastname: Nishikawa
authors.forename: Satoshi
authors.initials: S
authors.lastname: Nishikawa
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Arakawa