title: The N-terminus of FILIA forms an atypical KH domain with a unique extension involved in interaction with RNA.
abstract: FILIA is a member of the recently identified oocyte/embryo expressed gene family in eutherian mammals, which is characterized by containing an N-terminal atypical KH domain. Here we report the structure of the N-terminal fragment of FILIA (FILIA-N), which represents the first reported three-dimensional structure of a KH domain in the oocyte/embryo expressed gene family of proteins. The structure of FILIA-N revealed a unique N-terminal extension beyond the canonical KH region, which plays important roles in interaction with RNA. By co-incubation with the lysates of mice ovaries, FILIA and FILIA-N could sequester specific RNA components, supporting the critical roles of FILIA in regulation of RNA transcripts during mouse oogenesis and early embryogenesis.
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, Tianjin, China.
authors.forename: Juke
authors.initials: J
authors.lastname: Wang
authors.forename: Mengyuan
authors.initials: M
authors.lastname: Xu
authors.forename: Kai
authors.initials: K
authors.lastname: Zhu
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.forename: Xinqi
authors.initials: X
authors.lastname: Liu
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000378:metabolism; D002942:Circular Dichroism; D018360:Crystallography, X-Ray; D005260:Female; D051379:Mice; D000090682:Neuro-Oncological Ventral Antigen; D010053:Ovary / Q000378:metabolism; D011066:Poly C / Q000737:chemistry*; D011072:Poly U / Q000737:chemistry*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*; D012313:RNA / Q000737:chemistry* / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism; D014461:Ultracentrifugation
chemical_list: D000951:Antigens, Neoplasm; D000090682:Neuro-Oncological Ventral Antigen; C515624:Nova2 protein, mouse; D011506:Proteins; D016601:RNA-Binding Proteins; C532243:filia protein, mouse; D011072:Poly U; D011066:Poly C; D012313:RNA