title: Canadian macromolecular crystallography facility: a suite of fully automated beamlines.
abstract: The Canadian light source is a 2.9 GeV national synchrotron radiation facility located on the University of Saskatchewan campus in Saskatoon. The small-gap in-vacuum undulator illuminated beamline, 08ID-1, together with the bending magnet beamline, 08B1-1, constitute the Canadian Macromolecular Crystallography Facility (CMCF). The CMCF provides service to more than 50 Principal Investigators in Canada and the United States. Up to 25% of the beam time is devoted to commercial users and the general user program is guaranteed up to 55% of the useful beam time through a peer-review process. CMCF staff provides "Mail-In" crystallography service to users with the highest scored proposals. Both beamlines are equipped with very robust end-stations including on-axis visualization systems, Rayonix 300 CCD series detectors and Stanford-type robotic sample auto-mounters. MxDC, an in-house developed beamline control system, is integrated with a data processing module, AutoProcess, allowing full automation of data collection and data processing with minimal human intervention. Sample management and remote monitoring of experiments is enabled through interaction with a Laboratory Information Management System developed at the facility.
authors.affiliation: Candian Light Source Inc, 101 Perimeter Road, Saskatoon, SK, S7N 0X4, Canada. pawel.grochulski@lightsource.ca
authors.forename: Pawel
authors.initials: P
authors.lastname: Grochulski
authors.forename: Michel
authors.initials: M
authors.lastname: Fodje
authors.forename: Shaunivan
authors.initials: S
authors.lastname: Labiuk
authors.forename: James
authors.initials: J
authors.lastname: Gorin
authors.forename: Kathryn
authors.initials: K
authors.lastname: Janzen
authors.forename: Russ
authors.initials: R
authors.lastname: Berg
mesh_terms: D002170:Canada; D003461:Crystallography / Q000295:instrumentation* / Q000379:methods; D001330:Electronic Data Processing; D004926:Escherichia coli / Q000737:chemistry; D046911:Macromolecular Substances / Q000032:analysis* / Q000737:chemistry; D059346:Magnets; D018832:Molecular Chaperones / Q000737:chemistry / Q000302:isolation & purification; D010456:Peptides, Cyclic / Q000737:chemistry; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000737:chemistry; D017510:Protein Folding; D012371:Robotics / Q000295:instrumentation / Q000379:methods*; D012984:Software; D017356:Synchrotrons / Q000295:instrumentation
chemical_list: D046911:Macromolecular Substances; D018832:Molecular Chaperones; D010456:Peptides, Cyclic; D017853:Phosphotransferases (Alcohol Group Acceptor); C023132:glycerone kinase