title: [Protective function of melatonin to acute lung injury and its mechanisms in rats caused by oleic acid].
abstract: OBJECTIVE
  To observe the expression of P-selectin (Ps), intercellular adhesion molecule-1 (ICAM-1) and nuclear factor-kappa B (NF-kappaB) in lung tissues of acute lung injury (ALI) rat model induced by oleic acid (OA) and to explore the protective effects of melatonin (MT) in lung tissues in rats.
  
  
  METHODS
  All rats were randomly divided into four groups: control group, OA group, MT + OA group and SB203580 + OA group. Rat model of ALI was established by intravenous injection of oleic acid (OA). Lung coefficient was measured, lung tissues were imbedded by paraffin to observe morphological changes and the expression of Ps, ICAM-1 and NF-kappaB in lung tissues by means of immunohistochemistry staining.
  
  
  RESULTS
  Compared with control group, the lung coefficient increased significantly in OA group (P < 0.05). Alveolar septum thickened significantly in OA group, there had many infiltrated inflammatory cells and collapsed alveoli of lung; positive expression of Ps, ICAM-1 and NF-kappaB were very obvious (P < 0.05); the administration of MT and SB203580 mitigated above changes significantly (P < 0.05).
  
  
  CONCLUSIONS
  MT possesses obviously protective effect on lung tissues during ALI, its protective mechanism might be related to the inhibition of the expression of Ps, ICAM-1 and NF-kappaB.
authors.affiliation: Department of Pathophysiology, Hebei Medical University, Shijiazhuang 050017, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhang
authors.forename: Lei
authors.initials: L
authors.lastname: Gao
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Ding
authors.forename: Wen-Zhe
authors.initials: WZ
authors.lastname: Ma
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Gu
authors.forename: Yun-Lei
authors.initials: YL
authors.lastname: Ma
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000503:physiopathology* / Q000517:prevention & control*; D000818:Animals; D015536:Down-Regulation / Q000187:drug effects; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D008297:Male; D008550:Melatonin / Q000494:pharmacology* / Q000627:therapeutic use; D016328:NF-kappa B / Q000378:metabolism; D019301:Oleic Acid / Q000009:adverse effects; D019007:P-Selectin / Q000378:metabolism; D020011:Protective Agents / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D016328:NF-kappa B; D019007:P-Selectin; D020011:Protective Agents; D018799:Intercellular Adhesion Molecule-1; D019301:Oleic Acid; D008550:Melatonin