title: Measurement of cell death in mammalian cells.
abstract: This unit presents methods used to assess cell death in mammalian cells. The unit is divided into five sections: (1) a brief overview of cytotoxicity and pathways of cell death, (2) an improved method to measure cell death using lactate dehydrogenase (LDH) release as a marker of membrane integrity, (3) a flow cytometry method that simultaneously measures two types of cell death, oncosis and apoptosis, (4) use of nuclear morphology to assess apoptosis and oncosis, and (5) a brief discussion of the use of cytotoxicity assays to determine the mechanisms of cell death.
authors.affiliation: Medical University of South Carolina, Charleston, South Carolina, USA.
authors.forename: Brian S
authors.initials: BS
authors.lastname: Cummings
authors.forename: Rick G
authors.initials: RG
authors.lastname: Schnellmann
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001681:Biological Assay / Q000379:methods*; D015415:Biomarkers; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D064420:Drug-Related Side Effects and Adverse Reactions / Q000378:metabolism / Q000473:pathology*; D005434:Flow Cytometry / Q000379:methods; D007770:L-Lactate Dehydrogenase; D008322:Mammals; D009336:Necrosis / Q000139:chemically induced*; D013048:Specimen Handling
chemical_list: D015415:Biomarkers; D007770:L-Lactate Dehydrogenase