title: Formulation and in vitro characterization of PEGylated chitosan and polyethylene imine polymers with thrombospondin-I gene bearing pDNA.
abstract: An ideal gene carrier is required both in safety and efficiency for transfection. We examined the use of water soluble chitosan and polyethyleneimine as a carrier for anti-angiogenic protein, TSP-1 coded, in gene delivery. The aim of this study was to synthesize and characterize polyethylene glycol conjugated cationic polymers to increase anti-angiogenic gene transfection and reduce possible cytotoxicity. Gel electrophoresis study showed strong DNA binding ability of modified cationic polymers. Also structural properties of pegylated polymers were confirmed by (1)H-NMR. We investigated in vitro properties of PEG conjugated and coated particles which were observed between 145 and 250 nm with the positive zeta potential value. In addition, the chitosan-based DNA complexes did not induce remarkable cytotoxicity against MCF-7 cells. Due to low cytotoxicity, we observed high transfection efficiency at chitosan-based formulations compared with PEI ones. Although transfection studies carried on in vitro conditions, we measured slight increases at transfection with PEGylation. PEG-conjugated chitosan formulations can be a promising candidate due to its efficiency in condensing and transfection of pDNA, its low cytotoxicty and comparatively high encapsulation degree.
authors.affiliation: Department of Pharmaceutical Technology, Faculty of Pharmacy, Ankara University, Ankara, Turkey.
authors.forename: Ongun Mehmet
authors.initials: OM
authors.lastname: Saka
authors.forename: Asuman
authors.initials: A
authors.lastname: Bozkir
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D002412:Cations; D045744:Cell Line, Tumor; D048271:Chitosan / Q000737:chemistry*; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007097:Imines / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry; D010316:Particle Size; D010957:Plasmids / Q000378:metabolism; D020959:Polyethylene / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D019700:Thrombospondin 1 / Q000235:genetics*; D014162:Transfection
chemical_list: D001672:Biocompatible Materials; D002412:Cations; D007097:Imines; D011108:Polymers; D019700:Thrombospondin 1; D011092:Polyethylene Glycols; D020959:Polyethylene; D004247:DNA; D048271:Chitosan