title: Effect of autologous and allogenic platelet-rich plasma on human gingival fibroblast function.
abstract: OBJECTIVE
  Platelet-rich plasma (PRP) has been proposed as a method of delivering growth factors to enhance regeneration. The aim of this study was to investigate the use of autogenous and allogenic PRP and platelet-poor plasma (PPP) on migration and proliferation of human gingival fibroblasts in vitro.
  
  
  METHODS
  Various concentrations of PRP, as well as PPP, were prepared from autologous and allogenic sources and applied to primary gingival fibroblasts. Migration was determined by assessing the fibroblast response to a concentration gradient. (3)H-thymidine incorporation and crystal violet colorimetric assays were utilized to assess DNA synthesis and proliferation.
  
  
  RESULTS
  Platelet-rich plasma provides a significant migratory stimulus to gingival fibroblasts. Furthermore, the various concentrations of PRP (50%, 20% and 10%) do not promote DNA synthesis in the short term (24 h), but over the longer term (5 days) they stimulate an increase in cell proliferation. Compared with PPP, PRP was superior in terms of encouraging migration, but was inferior in terms of promoting DNA synthesis and cell proliferation. No difference was noted between the autologous and allogenic PRP preparations on cell function.
  
  
  CONCLUSIONS
  Both PPP and PRP promote gingival fibroblast migration and proliferation in vitro, without differences between preparations obtained from autologous and allogenic sources.
authors.affiliation: School of Dentistry, University of Queensland, Brisbane, QLD, Australia.
authors.forename: F
authors.initials: F
authors.lastname: Creeper
authors.forename: S
authors.initials: S
authors.lastname: Ivanovski
mesh_terms: D000704:Analysis of Variance; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology; D005881:Gingiva / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D006801:Humans; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology* / Q000502:physiology; D053657:Platelet-Rich Plasma / Q000502:physiology*; D018709:Statistics, Nonparametric
chemical_list: D010982:Platelet-Derived Growth Factor