title: Detection of Wolbachia in the tick Ixodes ricinus is due to the presence of the hymenoptera endoparasitoid Ixodiphagus hookeri.
abstract: The identification of micro-organisms carried by ticks is an important issue for human and animal health. In addition to their role as pathogen vectors, ticks are also the hosts for symbiotic bacteria whose impact on tick biology is poorly known. Among these, the bacterium Wolbachia pipientis has already been reported associated with Ixodes ricinus and other tick species. However, the origins of Wolbachia in ticks and their consequences on tick biology (known to be very diverse in invertebrates, ranging from nutritional symbionts in nematodes to reproductive manipulators in insects) are unknown. Here we report that the endoparasitoid wasp Ixodiphagus hookeri (Hymenoptera, Chalcidoidea, Encyrtidae)--strictly associated with ticks for their development--infested at almost 100% prevalence by a W. pipientis strain belonging to a Wolbachia supergroup that has already been reported as associated with other hymenopteran parasitoids. In a natural population of I. ricinus that suffers high parasitism rates due to I. hookeri, we used specific PCR primers for both hymenopteran and W. pipientis gene fragments to show that all unfed tick nymphs parasitized by I. hookeri also harbored Wolbachia, while unparasitized ticks were Wolbachia-free. We demonstrated experimentally that unfed nymphs obtained from larvae exposed to I. hookeri while gorging on their vertebrate host also harbor Wolbachia. We hypothesize that previous studies that have reported W. pipientis in ticks are due to the cryptic presence of the endoparasitoid wasp I. hookeri. This association has remained hidden until now because parasitoids within ticks cannot be detected until engorgement of the nymphs brings the wasp eggs out of diapause. Finally, we discuss the consequences of this finding for our understanding of the tick microbiome, and their possible role in horizontal gene transfer among pathogenic and symbiotic bacteria.
authors.affiliation: Biology, Epidemiology and Risk Analysis in Animal Health, (BioEpAR), INRA, UMR 1300, Nantes, France. olivier.plantard@oniris-nantes.fr
authors.forename: Olivier
authors.initials: O
authors.lastname: Plantard
authors.forename: Agnès
authors.initials: A
authors.lastname: Bouju-Albert
authors.forename: Marie-Astrid
authors.initials: MA
authors.lastname: Malard
authors.forename: Axelle
authors.initials: A
authors.lastname: Hermouet
authors.forename: Gilles
authors.initials: G
authors.lastname: Capron
authors.forename: Hélène
authors.initials: H
authors.lastname: Verheyden
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis / Q000235:genetics; D005260:Female; D020387:Food Chain; D006790:Host-Parasite Interactions / Q000235:genetics / Q000502:physiology*; D054884:Host-Pathogen Interactions / Q000235:genetics / Q000502:physiology; D006927:Hymenoptera / Q000382:microbiology* / Q000502:physiology; D018884:Ixodes / Q000382:microbiology* / Q000469:parasitology* / Q000502:physiology; D010802:Phylogeny; D013559:Symbiosis / Q000502:physiology; D013984:Tick Infestations / Q000382:microbiology; D020577:Wolbachia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology
chemical_list: D001425:Bacterial Outer Membrane Proteins; C112171:Wsp protein, Wolbachia