title: The rolB gene suppresses reactive oxygen species in transformed plant cells through the sustained activation of antioxidant defense.
abstract: The rolB (for rooting locus of Agrobacterium rhizogenes) oncogene has previously been identified as a key player in the formation of hairy roots during the plant-A. rhizogenes interaction. In this study, using single-cell assays based on confocal microscopy, we demonstrated reduced levels of reactive oxygen species (ROS) in rolB-expressing Rubia cordifolia, Panax ginseng, and Arabidopsis (Arabidopsis thaliana) cells. The expression of rolB was sufficient to inhibit excessive elevations of ROS induced by paraquat, menadione, and light stress and prevent cell death induced by chronic oxidative stress. In rolB-expressing cells, we detected the enhanced expression of antioxidant genes encoding cytosolic ascorbate peroxidase, catalase, and superoxide dismutase. We conclude that, similar to pathogenic determinants in other pathogenic bacteria, rolB suppresses ROS and plays a role not only in cell differentiation but also in ROS metabolism.
authors.affiliation: Institute of Biology and Soil Science, Far East Branch of Russian Academy of Sciences, Vladivostok 690022, Russia. bulgakov@ibss.dvo.ru
authors.forename: Victor P
authors.initials: VP
authors.lastname: Bulgakov
authors.forename: Tatiana Y
authors.initials: TY
authors.lastname: Gorpenchenko
authors.forename: Galina N
authors.initials: GN
authors.lastname: Veremeichik
authors.forename: Yuri N
authors.initials: YN
authors.lastname: Shkryl
authors.forename: Galina K
authors.initials: GK
authors.lastname: Tchernoded
authors.forename: Dmitry V
authors.initials: DV
authors.lastname: Bulgakov
authors.forename: Dmitry L
authors.initials: DL
authors.lastname: Aminin
authors.forename: Yuri N
authors.initials: YN
authors.lastname: Zhuravlev
mesh_terms: D060054:Agrobacterium / Q000235:genetics*; D000975:Antioxidants / Q000378:metabolism*; D017360:Arabidopsis / Q000166:cytology / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D060387:Ascorbate Peroxidases / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D016923:Cell Death; D002470:Cell Survival; D003470:Culture Media / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D008027:Light; D018384:Oxidative Stress; D005894:Panax / Q000166:cytology / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D010269:Paraquat / Q000494:pharmacology; D059828:Plant Cells / Q000187:drug effects / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D045470:Rubia / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D055051:Salt-Tolerant Plants / Q000166:cytology / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D024483:Vitamin K 3 / Q000494:pharmacology; D001617:beta-Glucosidase / Q000235:genetics / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D001426:Bacterial Proteins; D003470:Culture Media; D017382:Reactive Oxygen Species; D012965:Sodium Chloride; D024483:Vitamin K 3; D060387:Ascorbate Peroxidases; D013482:Superoxide Dismutase; C092241:RolB protein, Agrobacterium rhizogenes; D001617:beta-Glucosidase; D005978:Glutathione; D010269:Paraquat