title: In vitro enzymatic assays for Ser/Thr-selective protein kinases.
abstract: Medium and high throughput methods for measuring the activities of Ser/Thr-selective protein kinases are described. These methods utilize radiochemical detection, fluorescence polarization, and ultraviolet spectroscopy to monitor transfer of the gamma-phosphoryl group of ATP to protein or peptide substrates. These assays have utility in characterizing protein kinase inhibitors and in mechanistic studies.
authors.affiliation: GlaxoSmithKline, Research Triangle Park, North Carolina, USA.
authors.forename: H Neal
authors.initials: HN
authors.lastname: Bramson
mesh_terms: D001331:Automation; D001360:Avidin / Q000494:pharmacology; D001667:Binding, Competitive; D020536:Enzyme Activators / Q000494:pharmacology; D057075:Enzyme Assays / Q000379:methods*; D005454:Fluorescence Polarization / Q000379:methods; D006813:Humidity; D010766:Phosphorylation; D010770:Phosphotransferases / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000737:chemistry; D011505:Protein-Tyrosine Kinases / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000737:chemistry; D012588:Scintillation Counting / Q000379:methods; D013050:Spectrometry, Fluorescence / Q000379:methods; D013696:Temperature
chemical_list: D020536:Enzyme Activators; C093483:neutravidin; D001360:Avidin; D010770:Phosphotransferases; D011505:Protein-Tyrosine Kinases; D017346:Protein Serine-Threonine Kinases