title: Gastroprotective potential of frutalin, a D-galactose binding lectin, against ethanol-induced gastric lesions.
abstract: The present study was designed to verify whether frutalin (FTL) affords gastroprotection against the ethanol-induced gastric damage and to examine the underlying mechanism(s). Gastric damage was induced by intragastric administration of 0.2 ml of ethanol (96%). Mice in groups were pretreated with FTL (0.25, 0.5 and 1 mg/kg; i.p.), cimetidine (100 mg/kg; p.o.), or vehicle (0.9% of NaCl, 10 mL/kg; p.o.), 30 min before ethanol administration. They were sacrificed 30 min later, the stomachs excised, and the mucosal lesion area (mm²) measured by planimetry. Gastroprotection was assessed in relation to inhibition of gastric lesion area. To study the gastroprotective mechanism(s), its relations to capsaicin-sensitive fibers, endogenous prostaglandins, nitric oxide, sulphydryls, ATP-sensitive potassium channels, adrenoceptors, opioid receptors and calcium channels were analyzed. Treatments effects on ethanol-associated oxidative stress markers GSH and MDA were measured in gastric tissue. FTL afforded a dose-unrelated gastroprotection against the ethanol damage. However, it failed to prevent the ethanol-induced changes in the levels of GSH and MDA. It was observed that the gastroprotection by FTL was greatly reduced in animals pretreated with capsazepine, indomethacin, L-NAME or glibenclamide. Considering the results, it is suggested that the FTL could probably be a good therapeutic agent for the development of new medicine for the treatment of gastric ulcer.
authors.affiliation: Curso de Doutorado em Biotecnologia, Universidade de Fortaleza, RENORBIO, 60811-905 Fortaleza, CE, Brasil.
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: de Vasconcellos Abdon
authors.forename: Greicy
authors.initials: G
authors.lastname: Coelho de Souza
authors.forename: Lílian
authors.initials: L
authors.lastname: Noronha Coelho de Souza
authors.forename: Renata
authors.initials: R
authors.lastname: Prado Vasconcelos
authors.forename: Carolina
authors.initials: C
authors.lastname: Araújo Castro
authors.forename: Marjorie
authors.initials: M
authors.lastname: Moreira Guedes
authors.forename: Roberto César
authors.initials: RC
authors.lastname: Pereira Lima
authors.forename: Renato
authors.initials: R
authors.lastname: de Azevedo Moreira
authors.forename: Ana Cristina
authors.initials: AC
authors.lastname: de Oliveira Monteiro-Moreira
authors.forename: Adriana
authors.initials: A
authors.lastname: Rolim Campos
mesh_terms: D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology / Q000627:therapeutic use*; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use*; D015415:Biomarkers / Q000378:metabolism; D002211:Capsaicin / Q000031:analogs & derivatives / Q000494:pharmacology; D002927:Cimetidine / Q000627:therapeutic use; D000431:Ethanol; D005690:Galactose / Q000378:metabolism; D037161:Galectins / Q000494:pharmacology / Q000627:therapeutic use*; D005753:Gastric Mucosa / Q000187:drug effects* / Q000473:pathology; D005978:Glutathione / Q000378:metabolism; D005905:Glyburide / Q000494:pharmacology; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism
chemical_list: D000897:Anti-Ulcer Agents; D000975:Antioxidants; D015415:Biomarkers; D037161:Galectins; D010936:Plant Extracts; C570811:frutalin; D000431:Ethanol; D008315:Malondialdehyde; D002927:Cimetidine; D005978:Glutathione; C071423:capsazepine; D002211:Capsaicin; D005905:Glyburide; D019331:NG-Nitroarginine Methyl Ester; D005690:Galactose; D007213:Indomethacin