title: [Determination of serum vitamin B1 and B2 with HPLC].
abstract: OBJECTIVE
  To develop a HPLC method for the determination of serum vitamin B1 (VB1) and vitamin B2 (VB2) simultaneously.
  
  
  METHODS
  Serum samples were pretreated at ambient temperature on a C18 reversed phase chromatographic column, methanol plus phosphate buffer was used for mobile phase, vitamin B1 and B2 contents of serum samples eluted from a Sep-park C18 column were detected with a photodiode array detector. The influences of retention time, the volume and the proportion of eluent on the recovery of samples were investigated.
  
  
  RESULTS
  Recoveries were ranged from 82.67 to 96.89%. The coefficient of variation was < 10%. The linearity and correlation coefficient was 0.9985 and 0.9995, respectively. The contents of serum VB1 and VB2 in obese people were lower than those in healthy controls.
  
  
  CONCLUSIONS
  A HPLC method of determining serum VB1 and VB2 was established. Serum VB1 and VB2 level in obese people was low.
authors.affiliation: School of Public Health, Harbin Medical University, Harbin 150081, China. yanziliu2100@163.com
authors.forename: Liyan
authors.initials: L
authors.lastname: Liu
authors.forename: Changhao
authors.initials: C
authors.lastname: Sun
authors.forename: Cheng
authors.initials: C
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D009765:Obesity / Q000097:blood; D012256:Riboflavin / Q000097:blood*; D013831:Thiamine / Q000097:blood*
chemical_list: D012256:Riboflavin; D013831:Thiamine