title: Endothelial cell migration and morphogenesis on silk fibroin scaffolds containing hydroxyapatite electret.
abstract: The purpose of this study was to evaluate the effects of composite wound dressing films made of silk fibroin (SF) containing hydroxyapatite (HA) or polarized HA (pHA) powders on endothelial cell (EC) behaviors that have important roles in the wound-healing process. XRD revealed the SF films to be semicrystalline, with a broad peak centered at about 20.7° which is characteristic of β-sheets embedded within an amorphous matrix. The SF composite films with 0.6 (w/v)% in concentration of HA powder (HA/SF) or pHA powder (pHA/SF) contained HA crystals of amorphous and silk II crystalline structures. SEM observation showed that there were differences in SF morphology between HA/SF and pHA/SF. The pHA/SF exhibited a furry texture around the pHA crystals, most likely due to the stored charged and zeta potentials. The HA/SF and pHA/SF films enhanced EC migration compared with that on the SF film. The number of migrated cells on the HA/SF and pHA/SF was ~1.5 times larger than that on the SF. The quantitative analysis of the endothelial morphogenesis indicated that the pHA/SF film enhanced the formation of capillary-like structures compared with SF and HA/SF. Thus, pHA/SF may potentially stimulate and contribute to the enhancement of angiogenesis in the wound-healing process.
authors.affiliation: Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo, Japan. miho.bcr@tmd.ac.jp
authors.forename: Miho
authors.initials: M
authors.lastname: Nakamura
authors.forename: Tomoko
authors.initials: T
authors.lastname: Soya
authors.forename: Rumi
authors.initials: R
authors.lastname: Hiratai
authors.forename: Akiko
authors.initials: A
authors.lastname: Nagai
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Hashimoto
authors.forename: Ikuo
authors.initials: I
authors.lastname: Morita
authors.forename: Kimihiro
authors.initials: K
authors.lastname: Yamashita
mesh_terms: D000818:Animals; D002417:Cattle; D002465:Cell Movement; D002478:Cells, Cultured; D017886:Durapatite / Q000737:chemistry*; D004727:Endothelium / Q000166:cytology*; D005349:Fibroins / Q000737:chemistry*; D009024:Morphogenesis; D047011:Silk / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D047011:Silk; D005349:Fibroins; D017886:Durapatite