title: Inference of gene regulation via miRNAs during ES cell differentiation using MiRaGE method.
abstract: MicroRNA (miRNA) is a critical regulator of cell growth, differentiation, and development. To identify important miRNAs in a biological process, many bioinformatical tools have been developed. We have developed MiRaGE (MiRNA Ranking by Gene Expression) method to infer the regulation of gene expression by miRNAs from changes of gene expression profiles. The method does not require precedent array normalization. We applied the method to elucidate possibly important miRNAs during embryonic stem (ES) cell differentiation to neuronal cells and we infer that certain miRNAs, including miR-200 family, miR-429, miR-302 family, and miR-17-92 cluster members may be important to the maintenance of undifferentiated status in ES cells.
authors.affiliation: Department of Physics, Faculty of Science and Engineering, Chuo University, 1-13-27 Kasuga, Bunkyo-ku, Tokyo 112-8551, Japan. masa.yoshizawa44@gmail.com
authors.forename: Masato
authors.initials: M
authors.lastname: Yoshizawa
authors.forename: Y-H
authors.initials: YH
authors.lastname: Taguchi
authors.forename: Jun
authors.initials: J
authors.lastname: Yasuda
mesh_terms: D000465:Algorithms; D002454:Cell Differentiation; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008957:Models, Genetic; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism
chemical_list: D035683:MicroRNAs