title: Preventive effect of caffeine and curcumin on hepato-carcinogenesis             in diethylnitrosamine-induced rats.
abstract: Chemopreventive effects of caffeine and curcumin were evaluated in the diethylnitrosamine (DEN)-induced hepatocarcinogenic rat model. Animals injected with DEN for 10 weeks (G2-10w) and 14 weeks (G2-14w) were hepato-carcinogenic rats. Animals injected with DEN and treated with curcumin and caffeine for 10 weeks (G3-10w, G4-10w) and 14 weeks (G3-14w, G4-14w) were compared with those in G2. Macroscopic and microscopic features suggested that treatment with caffeine, but not curcumin, for 10 and 14 weeks was effective in inhibiting DEN-induced hepatocarcinogenesis. Immunohistochemical and western blot analysis with proliferating cell nuclear antigen and glutathione S-transferase-P antibodies also showed that expression levels of these hepato-carcinogenic markers were more efficiently reduced by treatment with caffeine than curcumin. Our data demonstrate that caffeine could be a more potent compound than curcumin for prevention of hepatocarcinogenesis in DEN-induced rats.
authors.affiliation: Second Department of Internal Medicine, Tottori University School                     of Medicine, Tottori 683-8504, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Fujise
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Okano
authors.forename: Takakazu
authors.initials: T
authors.lastname: Nagahara
authors.forename: Ryo
authors.initials: R
authors.lastname: Abe
authors.forename: Ryu
authors.initials: R
authors.lastname: Imamoto
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Murawaki
mesh_terms: D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D002110:Caffeine / Q000494:pharmacology / Q000627:therapeutic use*; D006528:Carcinoma, Hepatocellular / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D003474:Curcumin / Q000494:pharmacology / Q000627:therapeutic use*; D004052:Diethylnitrosamine; D051549:Glutathione S-Transferase pi / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008113:Liver Neoplasms / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D009929:Organ Size / Q000187:drug effects; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D000637:Transaminases / Q000097:blood
chemical_list: D016588:Anticarcinogenic Agents; D018809:Proliferating Cell Nuclear Antigen; D002110:Caffeine; D004052:Diethylnitrosamine; D051549:Glutathione S-Transferase pi; C496558:Gstp1 protein, rat; D000637:Transaminases; D003474:Curcumin