title: Human and bovine pulp-derived cell reactions  to dental resin cements.
abstract: OBJECTIVE
  The objective of this study was to evaluate the cytotoxic reaction of transfected human pulp derived cells (tHPDC) and transfected bovine pulp derived cells (tBPDC) after exposure to resin cements [RelyX UnicemClicker (RX), MaxCem (MC), Panavia F 2.0 (PF), BisCem (BC), and Bistite II DC (BII)] and to compare it to the generation of reactive oxygen species (ROS).
  
  
  METHODS
  Set materials were extracted in culture medium, cell survival as a measure of cytotoxicity was determined photometrically using crystal violet after cells were exposed to the extracts for 24 h. The generation of ROS was detected by flow cytometry after cells were exposed to extract dilutions for 1 h.
  
  
  RESULTS
  The ranking of the least to the most cytotoxic material was: RX < BII < PF < BC < MC for both cell lines, but for tHPDC, only MC and PF eluates were different from untreated controls. Generally, tBPDC were more susceptible to materials than tHPDC, but only for RX and BC was this difference statistically significant. All undiluted extracts increased ROS production in both cell lines but to a higher amount in tHPDC than in tBPDC.
  
  
  CONCLUSIONS
  tHPDC reacted less sensitive than tBPDC in the cytotoxicity test but with the same rank order of materials. In contrast, the cellular oxidative stress reaction was more pronounced in tHPDC than in tBPDC.
  
  
  CONCLUSIONS
  Depending on the residual dentine layer in deep cavities, biologically active resin monomers or additives released from resin cements may influence the dentine–pulp complex, for instance, its regenerative and reparative capacities.
authors.affiliation: Konya Oral Health Hospital, Konya Agiz Dis Sagligi Merkezi, 42070 Selcuklu, Konya, Turkey. botsalie@hotmail.com
authors.forename: Hayriye Esra
authors.initials: HE
authors.lastname: Ülker
authors.forename: Karl Anton
authors.initials: KA
authors.lastname: Hiller
authors.forename: Helmut
authors.initials: H
authors.lastname: Schweikl
authors.forename: Claudia
authors.initials: C
authors.lastname: Seidenader
authors.forename: Abdulkadir
authors.initials: A
authors.lastname: Sengun
authors.forename: Gottfried
authors.initials: G
authors.lastname: Schmalz
mesh_terms: D000818:Animals; D002417:Cattle; D018929:Cell Culture Techniques; D002461:Cell Line, Transformed; D002470:Cell Survival / Q000187:drug effects; D004396:Coloring Agents; D003188:Composite Resins / Q000633:toxicity; D017077:Culture Media, Conditioned; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects*; D005434:Flow Cytometry; D005452:Fluoresceins; D005456:Fluorescent Dyes; D005840:Gentian Violet; D006801:Humans; D008422:Materials Testing; D018384:Oxidative Stress / Q000187:drug effects; D010783:Photometry; D017382:Reactive Oxygen Species / Q000032:analysis; D019279:Resin Cements / Q000633:toxicity*; D013997:Time Factors; D014162:Transfection
chemical_list: C110400:2',7'-dichlorodihydrofluorescein diacetate; C542562:BisCem cement; C096475:Bistite Resin Cement; D004396:Coloring Agents; D003188:Composite Resins; D017077:Culture Media, Conditioned; D005452:Fluoresceins; D005456:Fluorescent Dyes; C515955:Maxcem; C438843:Panavia-Fluoro; D017382:Reactive Oxygen Species; C483701:Rely X Unicem; D019279:Resin Cements; D005840:Gentian Violet