title: Screening of different metal oxide nanoparticles reveals selective toxicity and inflammatory potential of silica nanoparticles in lung epithelial cells and macrophages.
abstract: In cell culture studies, foetal calf serum (FCS) comprising numerous different proteins is added, which might coat the surface of engineered nanomaterials (ENMs) and thus could profoundly alter their biological activities. In this study, a panel of industrially most relevant metal oxide nanoparticles (NPs) was screened for toxic effects in A549 lung epithelial cells and RAW264.7 macrophages in the presence and absence of FCS. In medium without FCS amorphous SiO2-NPs were the most cytotoxic NPs and induced a significant pro-inflammatory response in both cell types. An increased anti-oxidative response after exposure to SiO2-NPs was, however, only observed in RAW264.7 macrophages. Furthermore, pre-coating of SiO2-NPs with FCS proteins or simply bovine serum albumin abrogated responses in A549 lung epithelial cells. Thus, the protein corona bound to the surface of SiO2-NPs suppresses their biological effects, an issue which needs to be more carefully considered for in vitro-in vivo extrapolations.
authors.affiliation: Karlsruhe Institute of Technology, Campus North, Institute of Toxicology and Genetics, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Panas
authors.forename: C
authors.initials: C
authors.lastname: Marquardt
authors.forename: O
authors.initials: O
authors.lastname: Nalcaci
authors.forename: H
authors.initials: H
authors.lastname: Bockhorn
authors.forename: W
authors.initials: W
authors.lastname: Baumann
authors.forename: H-R
authors.initials: HR
authors.lastname: Paur
authors.forename: S
authors.initials: S
authors.lastname: Mülhopt
authors.forename: S
authors.initials: S
authors.lastname: Diabaté
authors.forename: C
authors.initials: C
authors.lastname: Weiss
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001798:Blood Proteins / Q000378:metabolism; D002417:Cattle; D002460:Cell Line; D002470:Cell Survival; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000139:chemically induced; D007378:Interleukins / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D053768:Metal Nanoparticles / Q000633:toxicity*; D051379:Mice; D010087:Oxides / Q000493:pharmacokinetics / Q000633:toxicity*; D010316:Particle Size; D017382:Reactive Oxygen Species / Q000378:metabolism; D020545:Respiratory Mucosa / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D012822:Silicon Dioxide / Q000493:pharmacokinetics / Q000633:toxicity*
chemical_list: D000975:Antioxidants; D001798:Blood Proteins; D007378:Interleukins; D010087:Oxides; D017382:Reactive Oxygen Species; D012822:Silicon Dioxide