title: Coordinated ciliary beating requires Odf2-mediated polarization of basal bodies via basal feet.
abstract: Coordinated beating of cilia in the trachea generates a directional flow of mucus required to clear the airways. Each cilium originates from a barrel-shaped basal body, from the side of which protrudes a structure known as the basal foot. We generated mice in which exons 6 and 7 of Odf2, encoding a basal body and centrosome-associated protein Odf2/cenexin, are disrupted. Although Odf2(ΔEx6,7/ΔEx6,7) mice form cilia, ciliary beating is uncoordinated, and the mice display a coughing/sneezing phenotype. Whereas residual expression of the C-terminal region of Odf2 in these mice is sufficient for ciliogenesis, the resulting basal bodies lack basal feet. Loss of basal feet in ciliated epithelia disrupted the polarized organization of apical microtubule lattice without affecting planar cell polarity. The requirement for Odf2 in basal foot formation, therefore, reveals a crucial role of this structure in the polarized alignment of basal bodies and coordinated ciliary beating.
authors.affiliation: Laboratory of Biological Science, Graduate School of Frontier Biosciences and Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan.
authors.forename: Koshi
authors.initials: K
authors.lastname: Kunimoto
authors.forename: Yuji
authors.initials: Y
authors.lastname: Yamazaki
authors.forename: Tomoki
authors.initials: T
authors.lastname: Nishida
authors.forename: Kyosuke
authors.initials: K
authors.lastname: Shinohara
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Ishikawa
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Hasegawa
authors.forename: Takeshi
authors.initials: T
authors.lastname: Okanoue
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hamada
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Noda
authors.forename: Atsushi
authors.initials: A
authors.lastname: Tamura
authors.forename: Shoichiro
authors.initials: S
authors.lastname: Tsukita
authors.forename: Sachiko
authors.initials: S
authors.lastname: Tsukita
mesh_terms: D000818:Animals; D002923:Cilia / Q000378:metabolism* / Q000502:physiology; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D007619:Kartagener Syndrome / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D008870:Microtubules / Q000378:metabolism; D012135:Respiratory Sounds / Q000502:physiology; D014132:Trachea / Q000502:physiology* / Q000648:ultrastructure*
chemical_list: D006360:Heat-Shock Proteins; C490106:Odf2 protein, mouse