title: Drug testing in cellular chemotaxis assays.
abstract: Described in this unit are methods for measuring the cell migration process. While cell adhesion protocols allow study of migrating cell interactions with the endothelial matrix, cellular migration assays permit analysis of directed cell movement towards a chemotactic gradient, both in vivo and in vitro. An in vitro cell invasion protocol is provided for analysis of the sum of the cell adhesion, migration, and invasion activities involved in tumor cell motility.
authors.affiliation: Department of Immunology and Oncology, Centro Nacional de Biotecnología/CSIC, Madrid, Spain.
authors.forename: Mario
authors.initials: M
authors.lastname: Mellado
authors.forename: Carlos
authors.initials: C
authors.lastname: Martínez-A
authors.forename: José Miguel
authors.initials: JM
authors.lastname: Rodríguez-Frade
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D002448:Cell Adhesion / Q000502:physiology*; D015815:Cell Adhesion Molecules / Q000494:pharmacology; D054443:Cell Migration Assays / Q000379:methods*; D002630:Chemotactic Factors / Q000494:pharmacology; D002633:Chemotaxis / Q000502:physiology*; D003094:Collagen / Q000494:pharmacology; D004338:Drug Combinations; D006801:Humans; D007797:Laminin / Q000494:pharmacology; D051379:Mice; D009361:Neoplasm Invasiveness / Q000473:pathology; D011509:Proteoglycans / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D001672:Biocompatible Materials; D015815:Cell Adhesion Molecules; D002630:Chemotactic Factors; D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C013065:cell aggregation factors; C059485:matrigel; D003094:Collagen