title: Input-dependent induction of oligonucleotide structural motifs for performing molecular logic.
abstract: The K(+)-H(+)-triggered structural conversion of multiple nucleic acid helices involving duplexes, triplexes, G-quadruplexes, and i-motifs is studied by gel electrophoresis, circular dichroism, and thermal denaturation. We employ the structural interconversions for perfoming molecular logic operations, as verified by fluorimetry and colorimetry. Short G-rich and C-rich cDNA and RNA single strands are hybridized to produce four A-form and B-form duplexes. Addition of K(+) triggers the unwinding of the duplexes by inducing the folding of G-rich strands into DNA- or RNA G-quadruplex mono- and multimers, respectively. We found a decrease in pH to have different consequences on the resulting structural output, depending on whether the C-rich strand is DNA or RNA: while the protonated C-rich DNA strand folds into at least two isomers of a stable i-motif structure, the protonated C-rich RNA strand binds a DNA/RNA hybrid duplex to form a Y·RY parallel triplex. When using K(+) and H(+) as external stimuli, or inputs, and the induced G-quadruplexes as reporters, these structural interconversions of nucleic acid helices can be employed for performing logic-gate operations. The signaling mode for detecting these conversions relies on complex formation between DNA or RNA G-quadruplexes (G4) and the cofactor hemin. The G4/hemin complexes catalyze the H(2)O(2)-mediated oxidation of peroxidase substrates, resulting in a fluorescence or color change. Depending on the nature of the respective peroxidase substrate, distinct output signals can be generated, allowing one to operate multiple logic gates such as NOR, INH, or AND.
authors.affiliation: Life and Medical Science Institute, Program Unit Chemical Biology and Medicinal Chemistry, University of Bonn, Gerhard-Domagk-Str. 1, 53121 Bonn, Germany.
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.forename: Damian
authors.initials: D
authors.lastname: Ackermann
authors.forename: Anna M
authors.initials: AM
authors.lastname: Hall
authors.forename: Michael
authors.initials: M
authors.lastname: Famulok
mesh_terms: D001483:Base Sequence; D002942:Circular Dichroism; D039301:Computers, Molecular; D004247:DNA / Q000737:chemistry*; D054856:G-Quadruplexes; D006859:Hydrogen / Q000737:chemistry; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009841:Oligonucleotides / Q000737:chemistry*; D011188:Potassium / Q000737:chemistry; D012313:RNA / Q000737:chemistry*
chemical_list: D009841:Oligonucleotides; D012313:RNA; D006859:Hydrogen; D004247:DNA; D011188:Potassium