title: Immobilization of enzyme on long period grating fibers for sensitive glucose detection.
abstract: Glucose oxidase (GOD) immobilized long period grating (LPG) fibers have been proposed for the specific and sensitive detection of glucose. The treatment of LPG fibers with aminopropyl triethoxysilane has induced biding sites for the subsequent GOD immobilization. Field emission scanning electron microscopy, confocal laser scanning microscopy, infrared spectroscopy and Raman spectroscopy have provided detailed evidences about the effectiveness of the adopted biofunctionalization methodology. The enzyme activity is conserved during the immobilization step. Fabricated LPG sensor was tested on different glucose solutions to record the transmission spectra on an optical spectrum analyzer. The wavelength shifts in the transmission spectra are linearly correlated with the glucose concentration in the range of 10-300 mg dL(-1). The fabricated sensor gives fast response and is demonstrated to be of practical utility by determining glucose contents in blood samples. Proposed technique can further be extended to develop LPG fiber based novel, sensitive and label free nanosensors for disease diagnosis and clinical analysis.
authors.affiliation: Central Scientific Instruments Organisation, Sector (CSIR-CSIO) 30 C, Chandigarh, India.
authors.forename: Akash
authors.initials: A
authors.lastname: Deep
authors.forename: Umesh
authors.initials: U
authors.lastname: Tiwari
authors.forename: Parveen
authors.initials: P
authors.lastname: Kumar
authors.forename: Vandana
authors.initials: V
authors.lastname: Mishra
authors.forename: Subhash C
authors.initials: SC
authors.lastname: Jain
authors.forename: Nahar
authors.initials: N
authors.lastname: Singh
authors.forename: Pawan
authors.initials: P
authors.lastname: Kapur
authors.forename: Lalit M
authors.initials: LM
authors.lastname: Bharadwaj
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry*; D006801:Humans; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman
chemical_list: D004800:Enzymes, Immobilized; D005949:Glucose Oxidase; D005947:Glucose