title: Tualang honey protects keratinocytes from ultraviolet radiation-induced inflammation and DNA damage.
abstract: Malaysian tualang honey possesses strong antioxidant and anti-inflammatory properties. Here, we evaluated the effect of tualang honey on early biomarkers of photocarcinogenesis employing PAM212 mouse keratinocyte cell line. Keratinocytes were treated with tualang honey (1.0%, v/v) before a single UVB (150 mJ cm(-2) ) irradiation. We found that the treatment of tualang honey inhibited UVB-induced DNA damage, and enhanced repair of UVB-mediated formation of cyclobutane pyrimidine dimers and 8-oxo-7,8-dihydro-2'-deoxyguanosine. Treatment of tualang honey inhibited UVB-induced nuclear translocation of NF-κB and degradation of IκBα in murine keratinocyte cell line. The treatment of tualang honey also inhibited UVB-induced inflammatory cytokines and inducible nitric oxide synthase protein expression. Furthermore, the treatment of tualang honey inhibited UVB-induced COX-2 expression and PGE2 production. Taken together, we provide evidence that the treatment of tualang honey to keratinocytes affords substantial protection from the adverse effects of UVB radiation via modulation in early biomarkers of photocarcinogenesis and provide suggestion for its photochemopreventive potential.
authors.affiliation: Department of Dermatology and Skin Diseases Research Center, Veterans Affairs Medical Center, Birmingham, AL, USA.
authors.forename: Israr
authors.initials: I
authors.lastname: Ahmad
authors.forename: Hugo
authors.initials: H
authors.lastname: Jimenez
authors.forename: Nik Soriani
authors.initials: NS
authors.lastname: Yaacob
authors.forename: Nabiha
authors.initials: N
authors.lastname: Yusuf
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D015415:Biomarkers / Q000378:metabolism; D002460:Cell Line; D004249:DNA Damage; D004260:DNA Repair / Q000187:drug effects* / Q000528:radiation effects; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects / Q000528:radiation effects; D006722:Honey; D020672:I-kappa B Proteins / Q000235:genetics / Q000378:metabolism; D007249:Inflammation / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects* / Q000528:radiation effects; D051379:Mice; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D011740:Pyrimidine Dimers / Q000737:chemistry / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects / Q000528:radiation effects; D012878:Skin Neoplasms / Q000517:prevention & control; D014466:Ultraviolet Rays
chemical_list: D000893:Anti-Inflammatory Agents; D015415:Biomarkers; D020672:I-kappa B Proteins; D016328:NF-kappa B; C000606205:Nfkbia protein, mouse; D011740:Pyrimidine Dimers; D000072000:NF-KappaB Inhibitor alpha; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine