title: Comparison of the methods for seeding human bone marrow mesenchymal stem cells to macroporous alginate cryogel carriers.
abstract: We performed a comparative study of the localization, distribution, metabolic activity, and surface properties of human bone marrow mesenchymal stromal cells after static and perfusion seeding to macroporous alginate cryogels. A simple perfusion system for mesenchymal stromal cell seeding to macroporous alginate cryogel sponges proposed in this study resulted in rapid and uniform distribution of cells within the whole volume of the scaffold preserving functional and morphological properties of the cells.
authors.affiliation: Institute of Cryobiology and Cryomedicine Problems, National Academy of Sciences of Ukraine, Khar'kov. yu.a.petrenko@gmail.com
authors.forename: Yu A
authors.initials: YA
authors.lastname: Petrenko
authors.forename: R V
authors.initials: RV
authors.lastname: Ivanov
authors.forename: V I
authors.initials: VI
authors.lastname: Lozinsky
authors.forename: A Yu
authors.initials: AY
authors.lastname: Petrenko
mesh_terms: D000464:Alginates; D001854:Bone Marrow Cells / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D002478:Cells, Cultured; D059985:Cryogels; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D000464:Alginates; D059985:Cryogels; D006603:Hexuronic Acids; D020723:Glucuronic Acid