title: Different expression of CD146 in human normal and osteosarcoma cell lines.
abstract: The CD146 cell membrane adhesion molecule is highly expressed on the cell surface of several tumours. The level of its expression has been found to correlate directly with tumour progression and metastatic potential, thus establishing CD146 as an important candidate of tumour growth and metastasis. In order to characterize its expression in human osteosarcoma (OS) cell lines, we have examined the CD146 expression at protein and RNA levels in both normal and tumour osteoblast-like cell lines by several methods. Our results indicate that CD146 protein is expressed at low levels in normal osteoblast cells whereas it is highly expressed in all OS cell lines analysed, (SaOS, MG-63, U-2OS). Moreover, CD146 overexpression was partially reduced in shYY1 cells, where the Yin Yang 1 transcription factor, also found over-expressed in human OS cells, has been silenced.
authors.affiliation: Department of General Pathology, Excellence Research Centre on Cardiovascular Disease, U.O.C. Immunohematology, Transfusion Medicine and Transplant Immunology [SIMT], Regional Reference Laboratory of Transplant Immunology [LIT], Azienda Universitaria Policlinico (AOU), 1st School of Medicine, Second University of Naples, Piazza Miraglia 2, 80138, Naples, Italy.
authors.forename: Concetta
authors.initials: C
authors.lastname: Schiano
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Grimaldi
authors.forename: Amelia
authors.initials: A
authors.lastname: Casamassimi
authors.forename: Teresa
authors.initials: T
authors.lastname: Infante
authors.forename: Alessandra
authors.initials: A
authors.lastname: Esposito
authors.forename: Alfonso
authors.initials: A
authors.lastname: Giovane
authors.forename: Claudio
authors.initials: C
authors.lastname: Napoli
mesh_terms: D001859:Bone Neoplasms / Q000737:chemistry*; D051929:CD146 Antigen / Q000032:analysis / Q000235:genetics; D045744:Cell Line, Tumor; D006801:Humans; D010006:Osteoblasts / Q000737:chemistry*; D012516:Osteosarcoma / Q000737:chemistry*
chemical_list: D051929:CD146 Antigen; C497902:MCAM protein, human