title: Controlled-release and preserved bioactivity of proteins from (self-assembled) core-shell double-walled microspheres.
abstract: In order to address preserved protein bioactivities and protein sustained-release problems, a method for preparing double-walled microspheres with a core (protein-loaded nanoparticles with a polymer-suspended granule system-formed core) and a second shell (a polymer-formed shell) for controlled drug release and preserved protein bioactivities has been developed using (solid-in-oil phase-in-hydrophilic oil-in-water (S/O/O(h)/W)) phases. The method, based on our previous microsphere preparation method (solid-in-oil phase-in-hydrophilic oil-in-water (S/O/O(h)/W), employs different concentric poly(D,L-lactide-co-glycolide), poly(D,L-lactide), and protein-loaded nanoparticles to produce a suspended liquid which then self-assembles to form shell-core microspheres in the hydrophilic oil phase, which are then solidified in the water phase. Variations in the preparation parameters allowed complete encapsulation by the shell phase, including the efficient formation of a poly(D,L-lactide) shell encapsulating a protein-loaded nanoparticle-based poly(D,L-lactide-co-glycolide) core. This method produces core-shell double-walled microspheres that show controlled protein release and preserved protein bioactivities for 60 days. Based upon these results, we concluded that the core-shell double-walled microspheres might be applied for tissue engineering and therapy for chronic diseases, etc.
authors.affiliation: Department of Neurology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai. yuanweien@126.com
authors.forename: Weien
authors.initials: W
authors.lastname: Yuan
authors.forename: Zhenguo
authors.initials: Z
authors.lastname: Liu
mesh_terms: D003692:Delayed-Action Preparations; D003911:Dextrans; D004337:Drug Carriers; D019344:Lactic Acid; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D009211:Myoglobin / Q000008:administration & dosage / Q000032:analysis / Q000737:chemistry; D053758:Nanoparticles / Q000032:analysis* / Q000648:ultrastructure; D010316:Particle Size; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011506:Proteins / Q000008:administration & dosage* / Q000032:analysis / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000008:administration & dosage / Q000032:analysis / Q000737:chemistry; D013499:Surface Properties; D013501:Surface-Active Agents / Q000737:chemistry / Q000494:pharmacology; D014783:Viscosity; D001616:beta-Galactosidase / Q000008:administration & dosage / Q000032:analysis / Q000737:chemistry
chemical_list: D003692:Delayed-Action Preparations; D003911:Dextrans; D004337:Drug Carriers; D009211:Myoglobin; D011506:Proteins; D013501:Surface-Active Agents; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D012710:Serum Albumin, Bovine; D019344:Lactic Acid; D001616:beta-Galactosidase