title: Effect of enterocin AS-48 in combination with biocides on planktonic and sessile Listeria monocytogenes.
abstract: Enterocin AS-48 was tested on a cocktail of Listeria monocytogenes strains in planktonic and sessile states, singly or in combination with biocides benzalkonium chloride, cetrimide, hexadecylpyridinium chloride, didecyldimethylammonium bromide, triclosan, poly-(hexamethylen guanidinium) hydrochloride, chlorhexidine, hexachlorophene, and the commercial sanitizers P3 oxonia and P3 topax 66. Combinations of sub-inhibitory bacteriocin concentrations and biocide concentrations 4 to 10-fold lower than their minimum inhibitory concentrations (MIC) completely inhibited growth of the planktonic listeriae. Inactivation of Listeria in biofilms formed on polystyrene microtiter plates required concentrations of enterocin AS-48 greater than 50 μg/ml, and biocide concentrations ten to 100-fold higher. In combination with enterocin AS-48 (25 or 50 μg/ml), microbial inactivation increased remarkably for all biocides except P3 oxonia and P3 topax 66 solutions. Polystyrene microtiter plates conditioned with enterocin solutions (0.5-25 μg/ml) decreased the adherence and biofilm formation of the L. monocytogenes cell cocktail, avoiding biofilm formation for at least 24 h at a bacteriocin concentration of 25 μg/ml.
authors.affiliation: Área de Microbiología, Departamento de Ciencias de la Salud, Facultad de Ciencias Experimentales, Edif. B3, Universidad de Jaén, Campus Las Lagunillas s/n. 23071-Jaén, Spain.
authors.forename: Natacha Caballero
authors.initials: NC
authors.lastname: Gómez
authors.forename: Hikmate
authors.initials: H
authors.lastname: Abriouel
authors.forename: M A José
authors.initials: MA
authors.lastname: Grande
authors.forename: Rubén Pérez
authors.initials: RP
authors.lastname: Pulido
authors.forename: Antonio
authors.initials: A
authors.lastname: Gálvez
mesh_terms: D001430:Bacteriocins / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development; D015169:Colony Count, Microbial; D004202:Disinfectants / Q000494:pharmacology*; D004338:Drug Combinations; D004357:Drug Synergism; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000254:growth & development / Q000472:pathogenicity*; D008826:Microbial Sensitivity Tests
chemical_list: D001430:Bacteriocins; D004202:Disinfectants; D004338:Drug Combinations