title: Effect of antioxidants resveratrol and quercetin on in vitro evaluation of frozen ram sperm.
abstract: The objective was to assess the effects of the antioxidants resveratrol and quercetin on frozen-thawed ram sperm. Semen samples (which exceeded minimum standards) from four mature crossbreed Santa Inês rams were pooled and aliquots of each pool were diluted in Tris-egg yolk-glycerol, with the addition of 0, 5, 10, 15, and 20 μg/mL of resveratrol and quercetin in Experiment 1 and Experiment 2, respectively. In Experiment 1, the proportion of sperm with a high mitochondrial membrane potential was greater (P < 0.02) in the control group than in resveratrol 20 μg/mL group. In Experiment 2, the proportion of sperm with high mitochondrial membrane potential was greater in the control group (P < 0.0001) than in the other experimental groups, and greater in the quercetin 5 μg/mL group (P < 0.05) than in the other quercetin-treated groups. Thus, addition of 5 to 20 μg/mL of either resveratrol or quercetin to the Tris-egg yolk-glycerol extender reduced sperm mitochondrial membrane potential.
authors.affiliation: Veterinary Medicine Department, Federal Rural University of Pernambuco, 52.171-900, Recife, Pernambuco, Brazil.
authors.forename: E C B
authors.initials: EC
authors.lastname: Silva
authors.forename: J F P
authors.initials: JF
authors.lastname: Cajueiro
authors.forename: S V
authors.initials: SV
authors.lastname: Silva
authors.forename: P C
authors.initials: PC
authors.lastname: Soares
authors.forename: M M P
authors.initials: MM
authors.lastname: Guerra
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D020011:Protective Agents / Q000494:pharmacology*; D011794:Quercetin / Q000494:pharmacology*; D000077185:Resveratrol; D055101:Semen Analysis / Q000662:veterinary; D012756:Sheep / Q000502:physiology*; D013094:Spermatozoa / Q000187:drug effects*; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D020011:Protective Agents; D013267:Stilbenes; D011794:Quercetin; D000077185:Resveratrol