title: Twin-beams digital holography for 3D tracking and quantitative phase-contrast microscopy in microfluidics.
abstract: We report on a compact twin-beam interferometer that can be adopted as a flexible diagnostic tool in microfluidic platforms with twofold functionality. The novel configuration allows 3D tracking of micro-particles and, at same time, can simultaneously furnish Quantitative Phase-contrast maps of tracked micro-objects by interference microscopy, without changing the configuration. Experimental demonstration is given on for in vitro cells in a microfluidic environment.
authors.affiliation: CNR - Istituto Nazionale di Ottica, via Campi Flegrei 34, Pozzuoli, Italy.
authors.forename: Pasquale
authors.initials: P
authors.lastname: Memmolo
authors.forename: Andrea
authors.initials: A
authors.lastname: Finizio
authors.forename: Melania
authors.initials: M
authors.lastname: Paturzo
authors.forename: Lisa
authors.initials: L
authors.lastname: Miccio
authors.forename: Pietro
authors.initials: P
authors.lastname: Ferraro
mesh_terms: D004867:Equipment Design; D019544:Equipment Failure Analysis; D006696:Holography / Q000295:instrumentation*; D007089:Image Enhancement / Q000295:instrumentation*; D007834:Lasers; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D008858:Microscopy, Phase-Contrast / Q000295:instrumentation*; D012815:Signal Processing, Computer-Assisted / Q000295:instrumentation*