title: Evaluation of a high-throughput diagnostic system for detection of HIV-1 in dried blood spot samples from infants in Mozambique.
abstract: We performed a comparative analysis between Roche Amplicor HIV-1 DNA test and CAPTAQ assay for the detection of HIV in 830 dried blood spot (DBS) pediatric samples collected in Mozambique. Our results demonstrated no statistical difference between these assays. The CAPTAQ assay approached nearly 100% repeatability/accuracy. The increased throughput of testing with minimal operator interference in performing the CAPTAQ assay clearly demonstrated that this method is an improvement over the Roche Amplicor HIV-1 DNA test, version 1.5.
authors.affiliation: Instituto Nacional de Saúde, Maputo, Mozambique.
authors.forename: Ilesh V
authors.initials: IV
authors.lastname: Jani
authors.forename: Jennifer
authors.initials: J
authors.lastname: Sabatier
authors.forename: Adolfo
authors.initials: A
authors.lastname: Vubil
authors.forename: Shambavi
authors.initials: S
authors.lastname: Subbarao
authors.forename: Dulce
authors.initials: D
authors.lastname: Bila
authors.forename: Amina
authors.initials: A
authors.lastname: de Sousa
authors.forename: Nédio
authors.initials: N
authors.lastname: Mabunda
authors.forename: Albert
authors.initials: A
authors.lastname: Garcia
authors.forename: Beth
authors.initials: B
authors.lastname: Skaggs
authors.forename: Dennis
authors.initials: D
authors.lastname: Ellenberger
authors.forename: Artur
authors.initials: A
authors.lastname: Ramos
mesh_terms: D059788:Dried Blood Spot Testing / Q000379:methods*; D015658:HIV Infections / Q000097:blood / Q000175:diagnosis*; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D007223:Infant; D025202:Molecular Diagnostic Techniques; D009073:Mozambique; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity