title: Plasmid DNA introduced into cultured cells with diagnostic ultrasound.
abstract: The usefulness of diagnostic ultrasound in gene transfer was investigated.             The hepatocellular carcinoma cell lines PRL/PRF/5 and Hep3B, and the pancreatic             carcinoma Panc-1 cells were transfected with lipofectin or irradiated with a linear             probe with a frequency of 8 MHz at a mechanical index of 0.4 through the bottom             of the plates for 5 min using diagnostic ultrasound (US) with pEGFP-N1 [green             fluorescent protein (GFP) expression plasmid], and observed under fluorescence             microscopy 48 h later. The cell lines were transfected or irradiated with US with             pGL3 control (luciferase reporter plasmid), and a luciferase assay (48 h later)             or an MTS assay (72 h later) were performed. Although signals of GFP were observed             in cells with US, the transfection efficiencies of US were lower than those of             transfection. Luciferase activities of cells with US were higher than those of             non-irradiated or transfected cells, but lower than those of transfection. Cell             viability with US did not change.
authors.affiliation: Department of Gastroenterology, National Hospital Organization Shimoshizu Hospital, 934-5 Shikawatashi, Yotsukaido City, Chiba 284-0003, Japan. nihminor-cib@umin.ac.jp
authors.forename: Minoru
authors.initials: M
authors.lastname: Tomizawa
authors.forename: Fuminobu
authors.initials: F
authors.lastname: Shinozaki
authors.forename: Takao
authors.initials: T
authors.lastname: Sugiyama
authors.forename: Shigenori
authors.initials: S
authors.lastname: Yamamoto
authors.forename: Makoto
authors.initials: M
authors.lastname: Sueishi
authors.forename: Takanobu
authors.initials: T
authors.lastname: Yoshida
mesh_terms: D045744:Cell Line, Tumor; D018014:Gene Transfer Techniques / Q000295:instrumentation; D006801:Humans; D010957:Plasmids / Q000528:radiation effects*; D013016:Sound; D014162:Transfection / Q000295:instrumentation / Q000379:methods*