title: Identification and expression analysis of IGFBP-1 gene from Japanese flounder (Paralichthys olivaceus).
abstract: Insulin-like growth factor binding protein-1 (IGFBP-1) plays an important role in IGF regulating vertebrate growth and development. In this study, we cloned IGFBP-1 cDNA from Japanese flounder (Paralichthys olivaceus) liver. The full-length cDNA is 1070 bp, including a 5'-untranslated region (UTR) of 69 bp, a 3'-UTR of 272 bp, and an open reading frame (ORF) of 729 bp encoding a polypeptide of 242 amino acids. Real-time quantitative PCR revealed that IGFBP-1 mRNA is mainly expressed in the liver, and a small amount of mRNAs was also found in other adult tissues. There are maternal transcripts of IGFBP-1 gene, and relatively low mRNA levels were observed in different embryonic stages. A higher level of IGFBP-1 mRNA was detected at 3 days post hatching (dph), and it got to the highest level at 29 dph (metamorphic climax), and finally brought back to a lower level at the end of metamorphosis. The expression of IGFBP-1 mRNA was greatly up-regulated in thyroid hormone (TH)-treated larvae, and declined after thiourea (TU) treatment. These results provide basic information for further studies on the role of IGF system in the P. olivaceus development and metamorphosis.
authors.affiliation: Key laboratory of Freshwater Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Agriculture, Shanghai 201306, PR China.
authors.forename: Wanying
authors.initials: W
authors.lastname: Zhai
authors.forename: Junling
authors.initials: J
authors.lastname: Zhang
authors.forename: Zhiyi
authors.initials: Z
authors.lastname: Shi
authors.forename: Yuanshuai
authors.initials: Y
authors.lastname: Fu
mesh_terms: D000595:Amino Acid Sequence; D000704:Analysis of Variance; D000818:Animals; D001483:Base Sequence; D002681:China; D003001:Cloning, Molecular; D016000:Cluster Analysis; D019295:Computational Biology; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D005432:Flounder; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000502:physiology*; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000235:genetics* / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013890:Thiourea / Q000494:pharmacology; D013963:Thyroid Hormones / Q000494:pharmacology
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D018970:Insulin-Like Growth Factor Binding Protein 1; D013963:Thyroid Hormones; D013890:Thiourea