title: PEG/RGD-modified magnetic polymeric liposomes for controlled drug release and tumor cell targeting.
abstract: Polymeric liposomes (PEG/RGD-MPLs), composed of amphiphilic polymer octadecyl-quaternized modified poly (γ-glutamic acid) (OQPGA), PEGylated OQPGA, RGD peptide grafted OQPGA and magnetic nanoparticles, was prepared successfully. These PEG/RGD-MPLs could be used as a multifunctional platform for targeted drug delivery. The results showed that PEG/RGD-MPLs were multilamellar spheres with nano-size (50-70 nm) and positive surface charge (28-42 mV). Compared with magnetic conventional liposomes (MCLs), PEG/RGD-MPLs exhibited sufficient size and zeta potential stability, low initial burst release and less magnetic nanoparticles leakage. The cell uptake results suggested that the PEG/RGD-MPLs (with RGD and magnetic particles) exhibited more drug cellular uptake than non RGD and non magnetism carriers in MCF-7 cells. MTT assay revealed that PEG/RGD-MPLs showed lower in vitro cytotoxicity to GES-1cells at ≤ 100 μg/mL. These data indicated that the multifunctional PEG/RGD-MPLs may be an alternative formulation for drug delivery system.
authors.affiliation: Institute of Nanobiotechnology, School of Materials Science and Engineering, Tianjin University, Tianjin 300072, PR China; Tianjin Key Laboratory of Composites and Functional Materials, Tianjin 300072, PR China.
authors.forename: Wenya
authors.initials: W
authors.lastname: Su
authors.affiliation: Institute of Nanobiotechnology, School of Materials Science and Engineering, Tianjin University, Tianjin 300072, PR China; Tianjin Key Laboratory of Composites and Functional Materials, Tianjin 300072, PR China.
authors.forename: Hanjie
authors.initials: H
authors.lastname: Wang
authors.affiliation: Institute of Nanobiotechnology, School of Materials Science and Engineering, Tianjin University, Tianjin 300072, PR China; Tianjin Key Laboratory of Composites and Functional Materials, Tianjin 300072, PR China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Wang
authors.affiliation: Institute of Nanobiotechnology, School of Materials Science and Engineering, Tianjin University, Tianjin 300072, PR China; Tianjin Key Laboratory of Composites and Functional Materials, Tianjin 300072, PR China.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Liao
authors.affiliation: Institute of Nanobiotechnology, School of Materials Science and Engineering, Tianjin University, Tianjin 300072, PR China; Tianjin Key Laboratory of Composites and Functional Materials, Tianjin 300072, PR China.
authors.forename: Shiyin
authors.initials: S
authors.lastname: Kang
authors.affiliation: Institute of Nanobiotechnology, School of Materials Science and Engineering, Tianjin University, Tianjin 300072, PR China; Tianjin Key Laboratory of Composites and Functional Materials, Tianjin 300072, PR China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Department of Neurosurgery, Laboratory of Neuro-Oncology, Tianjin Medical University General Hospital, Tianjin 300052, PR China. Electronic address: superhanlei@hotmail.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Han
authors.affiliation: Institute of Nanobiotechnology, School of Materials Science and Engineering, Tianjin University, Tianjin 300072, PR China; Tianjin Key Laboratory of Composites and Functional Materials, Tianjin 300072, PR China. Electronic address: jinchang@tju.edu.cn.
authors.forename: Jin
authors.initials: J
authors.lastname: Chang
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity; D001943:Breast Neoplasms / Q000378:metabolism*; D045744:Cell Line, Tumor; D002626:Chemistry, Pharmaceutical; D003692:Delayed-Action Preparations; D004339:Drug Compounding; D004355:Drug Stability; D015251:Epirubicin / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity; D005260:Female; D005753:Gastric Mucosa / Q000187:drug effects; D006801:Humans; D007700:Kinetics; D008055:Lipids / Q000737:chemistry* / Q000633:toxicity; D008081:Liposomes; D008280:Magnetics; D008823:Micelles; D015394:Molecular Structure; D053758:Nanoparticles; D036103:Nanotechnology; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry* / Q000633:toxicity; D011099:Polyglutamic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000633:toxicity; D000644:Quaternary Ammonium Compounds / Q000737:chemistry* / Q000633:toxicity; D012995:Solubility; D013499:Surface Properties; D013678:Technology, Pharmaceutical / Q000379:methods*
chemical_list: D000903:Antibiotics, Antineoplastic; D003692:Delayed-Action Preparations; D008055:Lipids; D008081:Liposomes; D008823:Micelles; D009842:Oligopeptides; D000644:Quaternary Ammonium Compounds; D011099:Polyglutamic Acid; D011092:Polyethylene Glycols; D015251:Epirubicin; C047981:arginyl-glycyl-aspartic acid