title: ZmHO-1, a maize haem oxygenase-1 gene, plays a role in determining lateral root development.
abstract: Previous results revealed that haem oxygenase-1 (HO-1)/carbon monoxide (CO) system is involved in auxin-induced adventitious root formation. In this report, a cDNA for the gene ZmHO-1, encoding an HO-1 protein, was cloned from Zea mays seedlings. ZmHO-1 has a conserved HO signature sequence and shares highest homology with rice SE5 (OsHO-1) protein. We further discovered that N-1-naphthylacetic acid (NAA), haemin, and CO aqueous solution, led to the induction of ZmHO-1 expression as well as the thereafter promotion of lateral root development. These effects were specific for ZmHO-1 since the potent HO-1 inhibitor zinc protoporphyrin IX (ZnPPIX) differentially blocked the above actions. The addition of haemin and CO were able to reverse the auxin depletion-triggered inhibition of lateral root formation as well as the decreased ZmHO-1 transcripts. Molecular evidence showed that the haemin- or CO-mediated the modulation of target genes responsible for lateral root formation, including ZmCDK and ZmCKI2, could be blocked by ZnPPIX. Overexpression of ZmHO-1 in transgenic Arabidopsis plants resulted in promotion of lateral root development as well as the modulation of cell cycle regulatory gene expressions. Overall, our results suggested that a maize HO-1 gene is required for the lateral root formation.
authors.affiliation: Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Hainan 571737, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Han
authors.forename: Sheng
authors.initials: S
authors.lastname: Xu
authors.forename: Yan-Jie
authors.initials: YJ
authors.lastname: Xie
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Huang
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Wang
authors.forename: Zheng
authors.initials: Z
authors.lastname: Yang
authors.forename: Chang-He
authors.initials: CH
authors.lastname: Zhang
authors.forename: Ya
authors.initials: Y
authors.lastname: Sun
authors.forename: Wen-Biao
authors.initials: WB
authors.lastname: Shen
authors.forename: Gui-Shui
authors.initials: GS
authors.lastname: Xie
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics; D002248:Carbon Monoxide / Q000494:pharmacology; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D015536:Down-Regulation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics*; D051547:Heme Oxygenase-1 / Q000235:genetics* / Q000378:metabolism; D006427:Hemin / Q000494:pharmacology; D007210:Indoleacetic Acids / Q000494:pharmacology; D008969:Molecular Sequence Data; D009280:Naphthaleneacetic Acids / Q000494:pharmacology; D010641:Phenotype; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*; D030821:Plants, Genetically Modified; D011524:Protoporphyrins / Q000494:pharmacology; D036226:Seedlings / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D017422:Sequence Analysis, DNA; D003313:Zea mays / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development
chemical_list: D018797:Cell Cycle Proteins; D004791:Enzyme Inhibitors; D007210:Indoleacetic Acids; D009280:Naphthaleneacetic Acids; D010940:Plant Proteins; D011524:Protoporphyrins; C017803:zinc protoporphyrin; C034182:1-naphthaleneacetic acid; D006427:Hemin; D002248:Carbon Monoxide; D051547:Heme Oxygenase-1