title: Transcription factor RORα is critical for nuocyte development.
abstract: Nuocytes are essential in innate type 2 immunity and contribute to the exacerbation of asthma responses. Here we found that nuocytes arose in the bone marrow and differentiated from common lymphoid progenitors, which indicates they are distinct, previously unknown members of the lymphoid lineage. Nuocytes required interleukin 7 (IL-7), IL-33 and Notch signaling for development in vitro. Pro-T cell progenitors at double-negative stage 1 (DN1) and DN2 maintained nuocyte potential in vitro, although the thymus was not essential for nuocyte development. Notably, the transcription factor RORα was critical for the development of nuocytes and their role in the expulsion of parasitic worms.
authors.affiliation: Medical Research Council Laboratory of Molecular Biology, Cambridge, UK.
authors.forename: See Heng
authors.initials: SH
authors.lastname: Wong
authors.forename: Jennifer A
authors.initials: JA
authors.lastname: Walker
authors.forename: Helen E
authors.initials: HE
authors.lastname: Jolin
authors.forename: Lesley F
authors.initials: LF
authors.lastname: Drynan
authors.forename: Emily
authors.initials: E
authors.lastname: Hams
authors.forename: Ana
authors.initials: A
authors.lastname: Camelo
authors.forename: Jillian L
authors.initials: JL
authors.lastname: Barlow
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Neill
authors.forename: Veera
authors.initials: V
authors.lastname: Panova
authors.forename: Ute
authors.initials: U
authors.lastname: Koch
authors.forename: Freddy
authors.initials: F
authors.lastname: Radtke
authors.forename: Clare S
authors.initials: CS
authors.lastname: Hardman
authors.forename: You Yi
authors.initials: YY
authors.lastname: Hwang
authors.forename: Padraic G
authors.initials: PG
authors.lastname: Fallon
authors.forename: Andrew N J
authors.initials: AN
authors.lastname: McKenzie
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D015851:Interleukin-7 / Q000276:immunology / Q000378:metabolism; D007962:Leukocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D009559:Nippostrongylus / Q000276:immunology; D057094:Nuclear Receptor Subfamily 1, Group F, Member 1 / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D015398:Signal Transduction; D017206:Strongylida Infections / Q000276:immunology; D060168:Thymocytes / Q000276:immunology
chemical_list: D015851:Interleukin-7; D057094:Nuclear Receptor Subfamily 1, Group F, Member 1; C539739:Rora protein, mouse