title: Emergence of OXA-48-type carbapenemase-producing Enterobacteriaceae in German hospitals.
abstract: Nine carbapenem-resistant Enterobacteriaceae isolates collected from eight patients in five German hospitals were investigated. Six isolates produced the OXA-48 carbapenemase, and three isolates produced OXA-162, which is a point mutant form of OXA-48. Both carbapenemase genes were located on IncL/M-type conjugative plasmids. Insertion sequence IS1999 (truncated or not by IS1R) was located upstream of the bla(OXA-48) and bla(OXA-162) genes in all of the isolates. Pulsed-field gel electrophoresis typing indicated the clonal transmission of an OXA-48-producing Klebsiella pneumoniae strain in two hospitals.
authors.affiliation: Robert Koch Institute, Nosocomial Infections, Wernigerode, Germany. pfeifery@rki.de
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Pfeifer
authors.forename: Kathrin
authors.initials: K
authors.lastname: Schlatterer
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Engelmann
authors.forename: Reinhold A
authors.initials: RA
authors.lastname: Schiller
authors.forename: Hans Reiner
authors.initials: HR
authors.lastname: Frangenberg
authors.forename: Doris
authors.initials: D
authors.lastname: Stiewe
authors.forename: Martin
authors.initials: M
authors.lastname: Holfelder
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Witte
authors.forename: Patrice
authors.initials: P
authors.lastname: Nordmann
authors.forename: Laurent
authors.initials: L
authors.lastname: Poirel
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D003227:Conjugation, Genetic; D003428:Cross Infection / Q000382:microbiology / Q000635:transmission; D004251:DNA Transposable Elements; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D004755:Enterobacteriaceae / Q000187:drug effects* / Q000201:enzymology*; D004756:Enterobacteriaceae Infections / Q000382:microbiology* / Q000635:transmission; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D005858:Germany; D006761:Hospitals; D006801:Humans; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D001618:beta-Lactamases; C063912:carbapenemase