title: A Seoul-Fluor-based bioprobe for lipid droplets and its application in image-based high throughput screening.
abstract: We developed a novel fluorescent bioprobe (SF44) that can specifically visualize the cellular lipid droplets in in vitro and in vivo systems and illustrated the mechanistic rationale of its fluorogenic property. Its application to image-based high throughput screening led us to the identification of a new small-molecule modulator of lipid droplet formation.
authors.affiliation: Department of Chemistry/Bio-MAX Institute, Seoul National University, Seoul 151-747, Korea.
authors.forename: Eunha
authors.initials: E
authors.lastname: Kim
authors.forename: Sanghee
authors.initials: S
authors.lastname: Lee
authors.forename: Seung Bum
authors.initials: SB
authors.lastname: Park
mesh_terms: D000818:Animals; D001896:Boron Compounds / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D006367:HeLa Cells; D006576:Heterocyclic Compounds, 4 or More Rings / Q000737:chemistry* / Q000494:pharmacology; D057166:High-Throughput Screening Assays; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008055:Lipids / Q000737:chemistry*; D051379:Mice; D015335:Molecular Probes / Q000737:chemistry* / Q000494:pharmacology; D010078:Oxazines / Q000737:chemistry
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D005227:Fatty Acids; D006576:Heterocyclic Compounds, 4 or More Rings; D008055:Lipids; D015335:Molecular Probes; D010078:Oxazines; C572124:SF44 compound; C044808:nile red