title: PEGylated click polypeptides synthesized by copper-free microwave-assisted thermal click polymerization for selective endotoxin removal from protein solutions.
abstract: PEGylated click polypeptides (PEG-CPs) containing α-amino side groups as well as PEG segments are designed for selective endotoxin removal from protein solutions. The PEG-CPs are synthesized via copper-free thermal click copolymerization from aspartic (or glutamic) acid-based dialkyne and diazide monomers (containing free amino side groups) and alkyne-terminated mPEGs or dialkyne-terminated PEGs. Microwave-assisting technology is introduced into thermal click chemistry to improve the reaction efficiency. The monomers and polymers are fully characterized using NMR, XPS, and MALDI-TOF MS. After immobilizing the PEGylated click polypeptides onto polystyrene microspheres, the adsorbents exhibit good endotoxin removal selectivity from BSA solutions.
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, No. 5625 Renmin Str., Changchun 130022, China.
authors.forename: Jinshan
authors.initials: J
authors.lastname: Guo
authors.forename: Fanbo
authors.initials: F
authors.lastname: Meng
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Li
authors.forename: Mingzhe
authors.initials: M
authors.lastname: Wang
authors.forename: Yanjuan
authors.initials: Y
authors.lastname: Wu
authors.forename: Xiabin
authors.initials: X
authors.lastname: Jing
authors.forename: Yubin
authors.initials: Y
authors.lastname: Huang
mesh_terms: D000480:Alkynes / Q000737:chemistry; D000818:Animals; D001386:Azides / Q000737:chemistry; D002417:Cattle; D057930:Click Chemistry; D004731:Endotoxins / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008863:Microspheres; D008872:Microwaves; D010455:Peptides / Q000138:chemical synthesis*; D056951:Photoelectron Spectroscopy; D011092:Polyethylene Glycols / Q000737:chemistry*; D058105:Polymerization; D011137:Polystyrenes; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000302:isolation & purification; D012996:Solutions; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000480:Alkynes; D001386:Azides; D004731:Endotoxins; D010455:Peptides; D011137:Polystyrenes; D012996:Solutions; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols