title: The onset of left ventricular diastolic dysfunction in SHR rats is not related to hypertrophy or hypertension.
abstract: Left ventricular (LV) diastolic dysfunction, particularly relaxation abnormalities, are known to be associated with the development of LV hypertrophy (LVH). Preliminary human and animal studies suggested that early LV diastolic dysfunction may be revealed independently of LVH. However, whether LV diastolic dysfunction is compromised before the onset of hypertension and LVH remains unknown. We therefore evaluated LV diastolic function in spontaneously hypertensive rats (SHR) at different ages and tested whether LV diastolic dysfunction is associated with abnormal intracellular calcium homeostasis. LV systolic and diastolic functions were evaluated by invasive and echocardiographic methods in 3-week-old (without hypertension) and 5-week-old (with hypertension) SHR and Wistar-Kyoto control rats. Basal intracytoplasmic calcium and sarcoplasmic reticulum (SR) Ca(2+) contents were measured in cardiomyocytes using fura-2 AM. Sarco(endo)plasmic Ca(2+)-ATPase isoform 2a (SERCA 2a) and phospholamban (PLB) expressions were quantified by Western blot and quantitative RT-PCR techniques. LV relaxation dysfunction was observed in 3-week-old SHR rats before onset of hypertension and LVH. An increase in basal intracytoplasmic Ca(2+) and a decrease in SR Ca(2+) release were demonstrated in SHR. Decreased expression of SERCA 2a and Ser16 PLB (p16-PLB) protein levels was also observed in SHR rats, whereas mRNA expression was not decreased. For the first time, we have shown that LV myocardial dysfunction precedes hypertension in 3-week-old SHR rats. This LV myocardial dysfunction was associated with high diastolic [Ca(2+)](i) possibly due to decreased SERCA 2a and p16-PLB protein levels. Diastolic dysfunction may be a potential predictive marker of arterial hypertension in genetic hypertension syndromes.
authors.affiliation: INSERM U, Amiens University Medical Center, France.
authors.forename: S
authors.initials: S
authors.lastname: Dupont
authors.forename: J
authors.initials: J
authors.lastname: Maizel
authors.forename: R
authors.initials: R
authors.lastname: Mentaverri
authors.forename: J-M
authors.initials: JM
authors.lastname: Chillon
authors.forename: I
authors.initials: I
authors.lastname: Six
authors.forename: P
authors.initials: P
authors.lastname: Giummelly
authors.forename: M
authors.initials: M
authors.lastname: Brazier
authors.forename: G
authors.initials: G
authors.lastname: Choukroun
authors.forename: C
authors.initials: C
authors.lastname: Tribouilloy
authors.forename: Z A
authors.initials: ZA
authors.lastname: Massy
authors.forename: M
authors.initials: M
authors.lastname: Slama
mesh_terms: D000758:Anesthesia; D000818:Animals; D001794:Blood Pressure / Q000502:physiology; D015153:Blotting, Western; D015220:Calcium Channels / Q000235:genetics / Q000502:physiology; D006332:Cardiomegaly / Q000150:complications / Q000503:physiopathology*; D003094:Collagen / Q000378:metabolism; D003326:Coronary Circulation / Q000502:physiology; D004452:Echocardiography; D015150:Echocardiography, Doppler; D005456:Fluorescent Dyes; D016257:Fura-2; D006973:Hypertension / Q000150:complications / Q000235:genetics / Q000503:physiopathology*; D066298:In Vitro Techniques; D008861:Microsomes / Q000187:drug effects; D032383:Myocytes, Cardiac / Q000187:drug effects; D012313:RNA / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D060888:Real-Time Polymerase Chain Reaction; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000096:biosynthesis / Q000235:genetics; D018487:Ventricular Dysfunction, Left / Q000209:etiology / Q000503:physiopathology*; D016277:Ventricular Function, Left / Q000502:physiology
chemical_list: D015220:Calcium Channels; D005456:Fluorescent Dyes; D012313:RNA; D003094:Collagen; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; D016257:Fura-2