title: A Case of Spontaneous Malignant Hibernoma in a Crl:CD(SD)IGS Rat.
abstract: A firm, tan, well-circumscribed mass that measured 25 × 30 × 35 mm was observed in the thoracic cavity of a 53-week-old male Crl:CD(SD) IGS rat. Histologically, the mass was encapsulated by fibrous tissue and contained fibrovascular septae. Tumor cells were compactly arranged, and most were oval to polygonal in shape with multivacuolated cytoplasm and a centrally located nucleus. In some parts of the tumor, marked cellular atypia and frequent mitoses were evident. Vacuoles in cytoplasm were positive for oil red O. The tumor cells were characterized ultrastructurally by abundant, round to oval mitochondria with transverse closely-packed cristae. Tumor cells were immunohistochemically positive for uncoupling protein 1 (UCP-1). Several thrombi and hemorrhagic or necrotic foci were also observed within the tumor mass. Vascular invasion of the tumor capsule was observed; however, invasion of surrounding tissues or metastases were not observed. Based on the pathology findings, this case was diagnosed as a malignant hibernoma.
authors.affiliation: Drug Safety Research Laboratories, Astellas Pharma Inc., 2-1-6 Kashima, Yodogawa-ku, Osaka 532-8514, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Anagawa
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Okazaki
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Murakami
authors.forename: Kenjiro
authors.initials: K
authors.lastname: Tsubota
authors.forename: Mihoko
authors.initials: M
authors.lastname: Ono
authors.forename: Masahiro
authors.initials: M
authors.lastname: Matsumoto
authors.forename: Shunji
authors.initials: S
authors.lastname: Nakatsuji
authors.forename: Yuji
authors.initials: Y
authors.lastname: Oishi