title: Identification of an E-box motif responsible for the expression of jasmonic acid-induced chitinase gene OsChia4a in rice.
abstract: The plant hormone jasmonic acid (JA) is known to be involved in multiple defence responses against pathogens, which include the production of pathogenesis-related (PR) proteins. In order to investigate the induction mechanism of the rice defence responses by JA, we performed transcriptome analyses and focused on a chitinase gene, OsChia4a, which was identified to be one of the highest JA-inductive genes. The recombinant protein of His-tagged OsChia4a exhibited an inhibitory effect against the spore germination and hyphal growth of Magnaporthe oryzae. The promoter analysis of OsChia4a revealed that the region from -515 bp to -265 bp upstream of the ATG translation initiation site was required for the responsiveness to JA. A subsequent mutation analysis indicated that an E-box (CANNTG) in this region act as a JA-responsive cis element. These results imply that a basic helix-loop-helix transcription factor is likely to be involved in the regulation of the OsChia4a expression in a JA-dependent manner.
authors.affiliation: Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Miyamoto
authors.forename: Takafumi
authors.initials: T
authors.lastname: Shimizu
authors.forename: Fengqiu
authors.initials: F
authors.lastname: Lin
authors.forename: Frank
authors.initials: F
authors.lastname: Sainsbury
authors.forename: Eva
authors.initials: E
authors.lastname: Thuenemann
authors.forename: George
authors.initials: G
authors.lastname: Lomonossoff
authors.forename: Hideaki
authors.initials: H
authors.lastname: Nojiri
authors.forename: Hisakazu
authors.initials: H
authors.lastname: Yamane
authors.forename: Kazunori
authors.initials: K
authors.lastname: Okada
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology; D020029:Base Pairing / Q000235:genetics; D001483:Base Sequence; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism; D002688:Chitinases / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology; D003517:Cyclopentanes / Q000494:pharmacology*; D024721:E-Box Elements / Q000235:genetics*; D004790:Enzyme Induction / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D017343:Genes, Plant / Q000235:genetics*; D020082:Magnaporthe / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D012275:Oryza / Q000166:cytology / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D054883:Oxylipins / Q000494:pharmacology*; D011401:Promoter Regions, Genetic / Q000235:genetics; D017384:Sequence Deletion
chemical_list: D000935:Antifungal Agents; D051792:Basic Helix-Loop-Helix Proteins; D002688:Chitinases; D003517:Cyclopentanes; D054883:Oxylipins; C011006:jasmonic acid