title: A non-allergenic Ole e 1-like protein from birch pollen as a tool to design hypoallergenic vaccine candidates.
abstract: Recombinant DNA technology offers several approaches to convert allergens into hypoallergenic derivatives that can represent the basis of novel, safer and more effective forms of allergy vaccines. In this context, we used a new strategy for the design of a hypoallergenic derivative of Ole e 1, the main allergen of olive pollen. By screening a cDNA library from birch pollen, the clone BB18, encoding the birch counterpart of Ole e 1, was identified. In this study, BB18 has been produce in Pichia pastoris as a recombinant protein and immunologically characterized. The well-established non-allergenic properties of BB18 were used to generate a genetic variant of Ole e 1, named OB(55-58), by site-direct mutagenesis of four residues (E(55)V(56)G(57)Y(58)) in an IgE/IgG epitope of Ole e 1 by the corresponding ones in BB18 (SDSE). OB(55-58) was expressed in P. pastoris, purified to homogeneity and analyzed for IgE-reactivity by means of ELISA using sera from olive pollen allergic patients and rat basophil activation assay. T cell reactivity was assayed in a mouse model of Ole e 1 sensitization. The mutant OB(55-58) exhibited an impaired IgE reactivity, but not affected T cell reactivity, compared to wild type rOle e 1. This study emphasizes the usefulness of BB18 as a tool for epitope mapping and for engineering hypoallergenic derivatives of Ole e 1 as vaccine candidates for allergy prevention and treatment.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de Química, Universidad Complutense, 28040 Madrid, Spain.
authors.forename: Eva G
authors.initials: EG
authors.lastname: Marazuela
authors.forename: Roswitha
authors.initials: R
authors.lastname: Hajek
authors.forename: Mayte
authors.initials: M
authors.lastname: Villalba
authors.forename: Domingo
authors.initials: D
authors.lastname: Barber
authors.forename: Heimo
authors.initials: H
authors.lastname: Breiteneder
authors.forename: Rosalía
authors.initials: R
authors.lastname: Rodríguez
authors.forename: Eva
authors.initials: E
authors.lastname: Batanero
mesh_terms: D000485:Allergens / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D052179:Antigens, Plant; D029662:Betula / Q000235:genetics / Q000378:metabolism; D015153:Blotting, Western; D003429:Cross Reactions / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D005260:Female; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology* / Q000517:prevention & control; D007073:Immunoglobulin E / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D031658:Olea / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D011058:Pollen / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D051381:Rats; D017386:Sequence Homology, Amino Acid; D014612:Vaccines / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000485:Allergens; D000911:Antibodies, Monoclonal; D052179:Antigens, Plant; D000939:Epitopes; D007074:Immunoglobulin G; C081946:Ole e I protein, Olea europaea; D010940:Plant Proteins; D014612:Vaccines; D007073:Immunoglobulin E