title: Analgesic, anti-inflammatory, and CNS depressant activities of new constituents of Nepeta clarkei.
abstract: Two new pentacyclic triterpenes named kirmanoic acid (1) and kurramanoic acid (2) have been isolated from the chloroform-soluble portion of the whole plant of Nepeta clarkei Hook. The structures of the two new compounds were assigned on the basis of their ¹H and ¹³C NMR spectra including two-dimensional NMR techniques such as COSY, HMQC, and HMBC experiments. Kirmanoic acid (1) was investigated for analgesic, anti-inflammatory, and CNS depressant activities. Interestingly kirmanoic acid (1) showed strong analgesic activity than standard drug in acetic induced writhing and formalin tests. Similarly kirmanoic acid (1) also showed strong anti-inflammatory activity than its standard drug. The gross behavioral study of kirmanoic acid (1) revealed that it exhibited mild CNS stimulant and muscle relaxant in the mice. Compound 1 showed a slight increase in Locomotor activity and possesses the antidepressant effect.
authors.affiliation: Department of Chemistry, Kohat University of Science and Technology, Kohat, Khyber Pakhtunkhwa, Pakistan. javidhej@yahoo.com
authors.forename: Javid
authors.initials: J
authors.lastname: Hussain
authors.forename: Najeeb
authors.initials: N
authors.lastname: Ur Rehman
authors.forename: Hidayat
authors.initials: H
authors.lastname: Hussain
authors.forename: Ahmed
authors.initials: A
authors.lastname: Al-Harrasi
authors.forename: Liaqat
authors.initials: L
authors.lastname: Ali
authors.forename: Tania Shamim
authors.initials: TS
authors.lastname: Rizvi
authors.forename: Mansoor
authors.initials: M
authors.lastname: Ahmad
authors.lastname: Mehjabeen
mesh_terms: D019342:Acetic Acid; D000700:Analgesics / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D000928:Antidepressive Agents / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D001522:Behavior, Animal / Q000187:drug effects*; D002492:Central Nervous System Depressants / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D000697:Central Nervous System Stimulants / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D005557:Formaldehyde; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D015394:Molecular Structure; D027521:Nepeta / Q000737:chemistry*; D009465:Neuromuscular Agents / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D010146:Pain / Q000139:chemically induced / Q000188:drug therapy; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D000700:Analgesics; D000893:Anti-Inflammatory Agents; D000928:Antidepressive Agents; D002492:Central Nervous System Depressants; D000697:Central Nervous System Stimulants; D009465:Neuromuscular Agents; D010936:Plant Extracts; D014315:Triterpenes; C574599:kirmanoic acid; D005557:Formaldehyde; D019342:Acetic Acid