title: Pseudomonas aeruginosa strain WI-1 from Mandovi estuary possesses metallothionein to alleviate lead toxicity and promotes plant growth.
abstract: A bacterial isolate from Mandovi estuary Goa, India, which can resist 0.6mM lead nitrate in Tris-buffered minimal medium was identified as Pseudomonas aeruginosa and designated as strain WI-1. PCR amplification clearly revealed presence of bmtA gene encoding bacterial metallothionein responsible for metal sequestration and AAS analysis proved intracellular bioaccumulation of 26.5mg lead/gram dry weight of cells. SDS-PAGE analysis confirmed lead induced bacterial metallothionein with molecular weight 11 kDa, which corresponds to the predicted bmtA gene. Significant growth inhibition of phytopathogenic fungi Fusarium oxysporum NCIM 1008 by siderophore-rich culture supernatant was also observed. Pot experiment with Pisum sativum L inoculated with this strain revealed higher seed germination percentage and significant growth promotion than uninoculated seeds in a soil amended with 7.704 g/kg lead, which indicates amelioration of lead toxicity. This lead resistant strain showed cross tolerance to cadmium, mercury and Tributyltin chloride (TBTC) along with resistance to multiple antibiotics.
authors.affiliation: Laboratory of Bacterial Genetics and Environmental Biotechnology, Department of Microbiology, Goa University, Taleigao Plateau, Goa 403 206, India.
authors.forename: Milind Mohan
authors.initials: MM
authors.lastname: Naik
authors.affiliation: Laboratory of Bacterial Genetics and Environmental Biotechnology, Department of Microbiology, Goa University, Taleigao Plateau, Goa 403 206, India.
authors.forename: Anju
authors.initials: A
authors.lastname: Pandey
authors.affiliation: Laboratory of Bacterial Genetics and Environmental Biotechnology, Department of Microbiology, Goa University, Taleigao Plateau, Goa 403 206, India. Electronic address: Santoshdubey.gu@gmail.com.
authors.forename: Santosh Kumar
authors.initials: SK
authors.lastname: Dubey
mesh_terms: D000898:Antibiosis; D001673:Biodegradation, Environmental; D004591:Electrophoresis, Polyacrylamide Gel; D005670:Fusarium / Q000502:physiology; D007194:India; D007854:Lead / Q000378:metabolism* / Q000633:toxicity; D008668:Metallothionein / Q000378:metabolism*; D063245:Plant Development; D010944:Plants / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification / Q000502:physiology*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
chemical_list: D012989:Soil Pollutants; D007854:Lead; D008668:Metallothionein