title: Differential DNA methylation status between human preadipocytes and mature adipocytes.
abstract: Obesity is a multifactorial disease resulting from interactions between susceptibility genes, psychosocial, and environmental factors. However, it is becoming evident that interindividual differences in obesity susceptibility depend also on epigenetic factors, although the mechanisms have not been fully elucidated. We have undertaken a genome-wide analysis of DNA methylation of human preadipocytes and mature adipocytes to examine the differences in methylation between them. We found hypomethylation occurring in 2,701 genes and hypermethylation in 1,070 genes after differentiation. Meanwhile, Gene Ontology analysis and Ingenuity Pathway Analysis showed many significant gene functions and pathways with altered methylation status after adipocyte differentiation. In addition, Signal-Net analysis showed that tumor necrosis factor-α, mitogen-activated protein kinase, and interleukin-8 were important to the formation of this network. Our results suggest that DNA methylation mechanisms may be involved in regulating the differentiation process of human preadipocytes.
authors.affiliation: Department of Pediatrics, Nanjing Maternal and Child Health Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Jin-Gai
authors.initials: JG
authors.lastname: Zhu
authors.forename: Li
authors.initials: L
authors.lastname: Xia
authors.forename: Chen-Bo
authors.initials: CB
authors.lastname: Ji
authors.forename: Chun-Mei
authors.initials: CM
authors.lastname: Zhang
authors.forename: Guan-Zhong
authors.initials: GZ
authors.lastname: Zhu
authors.forename: Chun-Mei
authors.initials: CM
authors.lastname: Shi
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.forename: Da-Ni
authors.initials: DN
authors.lastname: Qin
authors.forename: Xi-Rong
authors.initials: XR
authors.lastname: Guo
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D002454:Cell Differentiation; D019175:DNA Methylation; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism; D009765:Obesity / Q000378:metabolism / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
chemical_list: D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha; D020928:Mitogen-Activated Protein Kinases