title: Accumulation of endogenous LITAF in aggresomes.
abstract: LITAF is a 161 amino acid cellular protein which includes a proline rich N-terminus and a conserved C-terminal domain known as the simple-like domain. Mutations in LITAF have been identified in Charcot-Marie tooth disease, a disease characterized by protein aggregates. Cells transfected with cellular LITAF reveal that LITAF is localized to late endosomes/lysosomes. Here we investigated the intracellular localization of endogenous LITAF. We demonstrated that endogenous LITAF accumulates at a discrete cytoplasmic site in BGMK cells that we identify as the aggresome. To determine the domain within LITAF that is responsible for the localization of LITAF to aggresomes, we created a construct that contained the C-terminal simple-like domain of LITAF and found that this construct also localizes to aggresomes. These data suggest the simple-like domain is responsible for targeting endogenous LITAF to the aggresome.
authors.affiliation: Department of Biology, Trent University, Peterborough, Ontario, Canada.
authors.forename: Heather E
authors.initials: HE
authors.lastname: Eaton
authors.forename: Julie
authors.initials: J
authors.lastname: Metcalf
authors.forename: Andressa Ferreira
authors.initials: AF
authors.lastname: Lacerda
authors.forename: Craig R
authors.initials: CR
authors.lastname: Brunetti
mesh_terms: D000818:Animals; D002460:Cell Line; D002607:Charcot-Marie-Tooth Disease / Q000378:metabolism; D002522:Chlorocebus aethiops; D004268:DNA-Binding Proteins; D056827:Endosomal Sorting Complexes Required for Transport / Q000235:genetics / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006801:Humans; D051379:Mice; D046912:Multiprotein Complexes / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D000075702:Nedd4 Ubiquitin Protein Ligases; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D056827:Endosomal Sorting Complexes Required for Transport; C492727:Litaf protein, mouse; D046912:Multiprotein Complexes; D009687:Nuclear Proteins; D014157:Transcription Factors; D000075702:Nedd4 Ubiquitin Protein Ligases; D044767:Ubiquitin-Protein Ligases