title: Anti-inflammation effects of hydrogen saline in LPS activated macrophages and carrageenan induced paw oedema.
abstract: BACKGROUND
  Oxidative stress is thought to play an important role in the pathogenesis of inflammation. Recent studies have found that hydrogen gas has the effect of eliminating free radicals. Whether hydrogen saline (more convenient to be used than hydrogen gas) has the anti-inflammation effect or not is still unknown.
  
  
  METHODS
  Carrageenan-induced paw oedema and LPS-activated macrophages are studied in this article. Injection of carrageenan into the foot of a mouse elicited an acute inflammatory response characterized by increase of foot volume and infiltration of neutrophils. While tumor necrosis factorα(TNF-α) secreted by activated macrophages was determined by ELISA and real-time PCR.
  
  
  RESULTS
  All parameters of inflammation (foot volume, infiltration of neutrophils, amount of TNF-α and the level of TNF-α's mRNA) were attenuated by the hydrogen saline treatment.
  
  
  CONCLUSIONS
  As a more convenient way than inhaling H2, hydrogen saline exhibits a protective effect against inflammation and it might provide a novel therapeutic approach for inflammatory diseases.
authors.affiliation: Department of Nautical Medicine, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, P,R, of China. cljiang@vip.163.com.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Xu
authors.forename: Jiangrui
authors.initials: J
authors.lastname: Zhou
authors.forename: Jianmei
authors.initials: J
authors.lastname: Cai
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhu
authors.forename: Xuejun
authors.initials: X
authors.lastname: Sun
authors.forename: Chunlei
authors.initials: C
authors.lastname: Jiang