title: In vivo protein crystallization opens new routes in structural biology.
abstract: Protein crystallization in cells has been observed several times in nature. However, owing to their small size these crystals have not yet been used for X-ray crystallographic analysis. We prepared nano-sized in vivo-grown crystals of Trypanosoma brucei enzymes and applied the emerging method of free-electron laser-based serial femtosecond crystallography to record interpretable diffraction data. This combined approach will open new opportunities in structural systems biology.
authors.affiliation: Interfaculty Institute of Biochemistry, University of Tübingen, Tübingen, Germany.
authors.forename: Rudolf
authors.initials: R
authors.lastname: Koopmann
authors.forename: Karolina
authors.initials: K
authors.lastname: Cupelli
authors.forename: Lars
authors.initials: L
authors.lastname: Redecke
authors.forename: Karol
authors.initials: K
authors.lastname: Nass
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Deponte
authors.forename: Thomas A
authors.initials: TA
authors.lastname: White
authors.forename: Francesco
authors.initials: F
authors.lastname: Stellato
authors.forename: Dirk
authors.initials: D
authors.lastname: Rehders
authors.forename: Mengning
authors.initials: M
authors.lastname: Liang
authors.forename: Jakob
authors.initials: J
authors.lastname: Andreasson
authors.forename: Andrew
authors.initials: A
authors.lastname: Aquila
authors.forename: Sasa
authors.initials: S
authors.lastname: Bajt
authors.forename: Miriam
authors.initials: M
authors.lastname: Barthelmess
authors.forename: Anton
authors.initials: A
authors.lastname: Barty
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Bogan
authors.forename: Christoph
authors.initials: C
authors.lastname: Bostedt
authors.forename: Sébastien
authors.initials: S
authors.lastname: Boutet
authors.forename: John D
authors.initials: JD
authors.lastname: Bozek
authors.forename: Carl
authors.initials: C
authors.lastname: Caleman
authors.forename: Nicola
authors.initials: N
authors.lastname: Coppola
authors.forename: Jan
authors.initials: J
authors.lastname: Davidsson
authors.forename: R Bruce
authors.initials: RB
authors.lastname: Doak
authors.forename: Tomas
authors.initials: T
authors.lastname: Ekeberg
authors.forename: Sascha W
authors.initials: SW
authors.lastname: Epp
authors.forename: Benjamin
authors.initials: B
authors.lastname: Erk
authors.forename: Holger
authors.initials: H
authors.lastname: Fleckenstein
authors.forename: Lutz
authors.initials: L
authors.lastname: Foucar
authors.forename: Heinz
authors.initials: H
authors.lastname: Graafsma
authors.forename: Lars
authors.initials: L
authors.lastname: Gumprecht
authors.forename: Janos
authors.initials: J
authors.lastname: Hajdu
authors.forename: Christina Y
authors.initials: CY
authors.lastname: Hampton
authors.forename: Andreas
authors.initials: A
authors.lastname: Hartmann
authors.forename: Robert
authors.initials: R
authors.lastname: Hartmann
authors.forename: Günter
authors.initials: G
authors.lastname: Hauser
authors.forename: Helmut
authors.initials: H
authors.lastname: Hirsemann
authors.forename: Peter
authors.initials: P
authors.lastname: Holl
authors.forename: Mark S
authors.initials: MS
authors.lastname: Hunter
authors.forename: Stephan
authors.initials: S
authors.lastname: Kassemeyer
authors.forename: Richard A
authors.initials: RA
authors.lastname: Kirian
authors.forename: Lukas
authors.initials: L
authors.lastname: Lomb
authors.forename: Filipe R N C
authors.initials: FR
authors.lastname: Maia
authors.forename: Nils
authors.initials: N
authors.lastname: Kimmel
authors.forename: Andrew V
authors.initials: AV
authors.lastname: Martin
authors.forename: Marc
authors.initials: M
authors.lastname: Messerschmidt
authors.forename: Christian
authors.initials: C
authors.lastname: Reich
authors.forename: Daniel
authors.initials: D
authors.lastname: Rolles
authors.forename: Benedikt
authors.initials: B
authors.lastname: Rudek
authors.forename: Artem
authors.initials: A
authors.lastname: Rudenko
authors.forename: Ilme
authors.initials: I
authors.lastname: Schlichting
authors.forename: Joachim
authors.initials: J
authors.lastname: Schulz
authors.forename: M Marvin
authors.initials: MM
authors.lastname: Seibert
authors.forename: Robert L
authors.initials: RL
authors.lastname: Shoeman
authors.forename: Raymond G
authors.initials: RG
authors.lastname: Sierra
authors.forename: Heike
authors.initials: H
authors.lastname: Soltau
authors.forename: Stephan
authors.initials: S
authors.lastname: Stern
authors.forename: Lothar
authors.initials: L
authors.lastname: Strüder
authors.forename: Nicusor
authors.initials: N
authors.lastname: Timneanu
authors.forename: Joachim
authors.initials: J
authors.lastname: Ullrich
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Wang
authors.forename: Georg
authors.initials: G
authors.lastname: Weidenspointner
authors.forename: Uwe
authors.initials: U
authors.lastname: Weierstall
authors.forename: Garth J
authors.initials: GJ
authors.lastname: Williams
authors.forename: Cornelia B
authors.initials: CB
authors.lastname: Wunderer
authors.forename: Petra
authors.initials: P
authors.lastname: Fromme
authors.forename: John C H
authors.initials: JC
authors.lastname: Spence
authors.forename: Thilo
authors.initials: T
authors.lastname: Stehle
authors.forename: Henry N
authors.initials: HN
authors.lastname: Chapman
authors.forename: Christian
authors.initials: C
authors.lastname: Betzel
authors.forename: Michael
authors.initials: M
authors.lastname: Duszenko
mesh_terms: D003461:Crystallography / Q000379:methods*; D018360:Crystallography, X-Ray / Q000379:methods*; D011485:Protein Binding / Q000528:radiation effects; D011487:Protein Conformation / Q000528:radiation effects; D011506:Proteins / Q000737:chemistry* / Q000528:radiation effects / Q000648:ultrastructure*; D012995:Solubility / Q000528:radiation effects; D014965:X-Rays
chemical_list: D011506:Proteins