title: Effect of humoral factors from hPDLSCs on the biologic activity of hABCs.
abstract: OBJECTIVE
  The human periodontal ligament stem cells (hPDLSCs) and human alveolar bone-derived stromal cells (hABCs) seem to be closely involved in the maintenance of alveolar bone in an anatomically indirect manner; however, there is little study on this matter. Therefore, the effect of hPDLSCs on the osteoclastogenic, osteogenic, and adipogenic differentiation of hABCs was evaluated, focusing on the humoral factors released by hPDLSCs.
  
  
  METHODS
  Human periodontal ligament stem cells and hABCs were isolated and characterized. hPDLSCs were indirectly cocultured to observe the in vitro effect of humoral factors released from hPDLSCs on the osteoclastogenic, osteogenic, and adipogenic differentiation of hABCs. Human gingival fibroblasts (hGFs) were utilized as positive control.
  
  
  RESULTS
  Isolated cells demonstrated the presence of stem cells within. Indirect coculture of hPDLSCs greatly inhibited osteoclastogenesis by hABCs. Osteogenesis/adipogenesis of hABCs was also inhibited by indirect coculture with hPDLSC. The magnitude of regulatory effect from hPDLSCs was significantly greater than that of hGFs.
  
  
  CONCLUSIONS
  Humoral factors released from hPDLSCs seemed to modulate the differentiation of hABCs, and the osteoclastogenic, osteogenic, and adipogenic differentiation of hABCs was all inhibited, suggesting the potential role of hPDLSCs in the maintenance of the alveolar bone.
authors.affiliation: Department of Periodontology, Research Institute for Periodontal Regeneration, College of Dentistry, Yonsei University, Seoul, Korea.
authors.forename: J-C
authors.initials: JC
authors.lastname: Park
authors.forename: S-M
authors.initials: SM
authors.lastname: Lee
authors.forename: J C
authors.initials: JC
authors.lastname: Kim
authors.forename: J-H
authors.initials: JH
authors.lastname: Yun
authors.forename: K-S
authors.initials: KS
authors.lastname: Cho
authors.forename: G-I
authors.initials: GI
authors.lastname: Im
authors.forename: B-S
authors.initials: BS
authors.lastname: Kim
authors.forename: C-S
authors.initials: CS
authors.lastname: Kim
mesh_terms: D017667:Adipocytes / Q000502:physiology; D050156:Adipogenesis / Q000502:physiology; D000293:Adolescent; D000328:Adult; D000539:Alveolar Process / Q000166:cytology*; D000818:Animals; D002452:Cell Count; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D002469:Cell Separation; D018920:Coculture Techniques; D003470:Culture Media; D005347:Fibroblasts / Q000502:physiology; D005881:Gingiva / Q000166:cytology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000502:physiology; D051379:Mice; D016513:Mice, SCID; D010006:Osteoblasts / Q000502:physiology; D010010:Osteoclasts / Q000502:physiology; D010012:Osteogenesis / Q000502:physiology; D019899:Paracrine Communication / Q000502:physiology*; D010513:Periodontal Ligament / Q000166:cytology*; D013234:Stem Cells / Q000502:physiology*; D017154:Stromal Cells / Q000502:physiology*; D055815:Young Adult
chemical_list: D003470:Culture Media