title: Location and Density of Immune Cells in Precursor Lesions and Cervical Cancer.
abstract: Only a small proportion of women infected with Human Papillomavirus (HPV) develop cervical cancer. Host immune response seems to play a role eliminating the viral infection and preventing progression to cancer. Characterization of tumor infiltrating lymphocytes (TILs) in cervical pre-neoplastic lesions and cervical cancer may be helpful to understand the mechanisms that mediate this protection. The aim of this study was to determine if there are differences in the localization and density (cells/mm(2)) of CD8+ T-cells, CD4+ T-cells and Tregs (CD25 + Foxp3+) in cervical pre-neoplastic lesions and cervical cancer. Immunohistochemical analysis of sections of 96 (26 CIN1, 21 CIN2, 25 CIN3, and 24 SCC) samples revealed that regardless of CIN grades, CD8+ T-cells are more abundant than CD4+, CD25+ and Foxp3+ cells in both the stroma and epithelium. There was a higher density of CD8+ cells in the stroma of cervical cancer compared to CIN3 (OR = 4.20, 95% CI 1.2-15), CIN2 (OR = 7.86, 95% CI 1.7-36.4) and CIN1 (OR = 4.25, 95% CI 1.1-17). Studies evaluating whether these cells are recruited before or after cancer progression will be helpful to understand the role of these cells in the natural history of HPV-induced lesions.
authors.affiliation: Grupo Infección y Cáncer, Facultad de Medicina, Universidad de Antioquia, Cra 51D No. 62-29 Lab 283, Medellin, Colombia.|Escuela de Microbiología, Universidad de Antioquia, Medellin, Colombia.
authors.forename: Astrid M
authors.initials: AM
authors.lastname: Bedoya
authors.affiliation: Laboratorio de Patología, Fundación Valle del Lili, Cali, Colombia.
authors.forename: Roberto
authors.initials: R
authors.lastname: Jaramillo
authors.affiliation: Grupo Infección y Cáncer, Facultad de Medicina, Universidad de Antioquia, Cra 51D No. 62-29 Lab 283, Medellin, Colombia.|Escuela de Microbiología, Universidad de Antioquia, Medellin, Colombia.
authors.forename: Armando
authors.initials: A
authors.lastname: Baena
authors.affiliation: Departamento de Patología, Facultad de Medicina, Universidad de Antioquia and Hospital Universitario San Vicente de Paúl, Medellin, Colombia.
authors.forename: Jorge
authors.initials: J
authors.lastname: Castaño
authors.affiliation: Departamento de Patología, Facultad de Medicina, Universidad de Antioquia and Hospital Universitario San Vicente de Paúl, Medellin, Colombia.
authors.forename: Natalia
authors.initials: N
authors.lastname: Olaya
authors.affiliation: Department of Microbiology, Stanley S. Scott Cancer Center, Louisiana State University Health Sciences Center, New Orleans, LA, USA.
authors.forename: Arnold H
authors.initials: AH
authors.lastname: Zea
authors.affiliation: Prevention and Implementation Group, International Agency for Research on Cancer, Lyon, France.
authors.forename: Rolando
authors.initials: R
authors.lastname: Herrero
authors.affiliation: Grupo Infección y Cáncer, Facultad de Medicina, Universidad de Antioquia, Cra 51D No. 62-29 Lab 283, Medellin, Colombia. sanchezg@medicina.udea.edu.co.
authors.forename: Gloria I
authors.initials: GI
authors.lastname: Sanchez