title: Detection of adventitious viruses from biologicals using a broad-spectrum microbial detection array.
abstract: CONFERENCE PROCEEDING Proceedings of the PDA/FDA Adventitious Viruses in Biologics: Detection and Mitigation Strategies Workshop in Bethesda, MD, USA; December 1-3, 2010 Guest Editors: Arifa Khan (Bethesda, MD), Patricia Hughes (Bethesda, MD) and Michael Wiebe (San Francisco, CA) We designed the Lawrence Livermore Microbial Detection Array (LLMDA), which contains 388,000 DNA probes. This array can detect any sequenced viruses or bacteria within 24 h. In addition, the oligonucleotide probes were selected to enable detection of novel, divergent species with homology to sequenced organisms. We recently used this array to identify an adventitious virus from a vaccine product. We have also used this array to detect viral and bacterial infections from various human clinical samples. Broad-spectrum microbial detection microarrays are efficient and cost-effective tools to rapidly screen cell bank samples, raw materials, vaccine samples, and clinical samples to ensure drug, food, and health safety in the United States and worldwide.
authors.affiliation: Physical & Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA.
authors.forename: Crystal
authors.initials: C
authors.lastname: Jaing
authors.forename: Shea
authors.initials: S
authors.lastname: Gardner
authors.forename: Kevin
authors.initials: K
authors.lastname: McLoughlin
authors.forename: James B
authors.initials: JB
authors.lastname: Thissen
authors.forename: Tom
authors.initials: T
authors.lastname: Slezak
mesh_terms: D001419:Bacteria / Q000235:genetics; D001424:Bacterial Infections / Q000382:microbiology; D015342:DNA Probes; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D015345:Oligonucleotide Probes / Q000235:genetics; D014481:United States; D014486:United States Food and Drug Administration; D014780:Viruses / Q000235:genetics
chemical_list: D015342:DNA Probes; D015345:Oligonucleotide Probes