title: Secreted 70kDa lumican stimulates growth and inhibits invasion of human pancreatic cancer.
abstract: Lumican expression in the stromal tissues of pancreatic ductal adenocarcinoma (PDAC) correlates with tumor invasion, and tends to correlate with poor prognosis. We used gene transfection techniques to examine the biological roles of lumican secreted from PDAC cells. Lumican-transfected PANC-1 cells secreted a 70-kDa lumican protein and had an active ERK pathway. Transfection stimulated PANC-1 cell growth, increased cell adhesion to laminin, inhibited cell invasion, and decreased active matrix metalloproteinase-9. Down-regulation of lumican using siRNA resulted in opposite cell behavior. Thus, the 70-kDa lumican secreted by PDAC cells plays important roles in cell growth and invasion.
authors.affiliation: Departments of Pathology and Integrative Oncological Pathology, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8602, Japan.
authors.forename: Tetsushi
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Yoko
authors.initials: Y
authors.lastname: Matsuda
authors.forename: Kiyoko
authors.initials: K
authors.lastname: Kawahara
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Ishiwata
authors.forename: Zenya
authors.initials: Z
authors.lastname: Naito
mesh_terms: D021441:Carcinoma, Pancreatic Ductal / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D048708:Cell Growth Processes / Q000502:physiology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D011508:Chondroitin Sulfate Proteoglycans / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D006031:Glycosylation; D006801:Humans; D039422:Integrin alpha3 / Q000096:biosynthesis; D007632:Keratan Sulfate / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D000071234:Lumican; D020935:MAP Kinase Signaling System; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D009361:Neoplasm Invasiveness; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D034741:RNA, Small Interfering / Q000008:administration & dosage / Q000235:genetics; D014162:Transfection
chemical_list: D011508:Chondroitin Sulfate Proteoglycans; D039422:Integrin alpha3; C000604306:LUM protein, human; D000071234:Lumican; D034741:RNA, Small Interfering; D007632:Keratan Sulfate; D051057:Proto-Oncogene Proteins c-akt; D048049:Extracellular Signal-Regulated MAP Kinases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9