title: Non-association of Crohn's disease with NOD2 gene variants in Moroccan patients.
abstract: Crohn's disease is a chronic inflammatory bowel disease, with multifactorial traits, that can involve any part of the gastrointestinal tract. In recent years, a dozen genome-wide association scan and meta-analysis were published bringing the number of susceptibility alleles to more than 30 variations. However, the major susceptibility gene for Crohn's disease is NOD2, located on proximal 16q, which is involved in the innate immune response. Three main variants of this gene: two single nucleotide polymorphisms p.Arg702Trp and p.Gly908Arg substitutions and frameshift polymorphism p.Leu1007fsinsC are involved in susceptibility to Crohn's disease. There is no data about the frequency of these allelic variants in Moroccan patients with Crohn's disease. The aim of our study is to genotype the NOD2 gene to assess the involvement of these three variants in susceptibility to Crohn's disease for Moroccans.
  
  
  METHODS
  We carried out genotyping for the three variants p.Arg702Trp, p.Gly908Arg and p.Leu1007fsinsC of NOD2 gene using PCR-sequencing among 101 Moroccan patients with Crohn's disease and 107 healthy controls.
  
  
  RESULTS
  The three main variants of NOD2 gene were present in Moroccan patients with no significant difference compared to controls.
  
  
  CONCLUSIONS
  This preliminary study shows no evidence association of NOD2 gene with Crohn's disease in the Moroccan population.
authors.affiliation: Centre de Génomique Humaine, Faculté de Médecine et de Pharmacie, Université Mohamed V Souissi, Rabat, Morocco. iman.hama@yahoo.fr
authors.forename: I
authors.initials: I
authors.lastname: Hama
authors.forename: I
authors.initials: I
authors.lastname: Ratbi
authors.forename: S
authors.initials: S
authors.lastname: Reggoug
authors.forename: F
authors.initials: F
authors.lastname: Elkerch
authors.forename: G
authors.initials: G
authors.lastname: Kharrasse
authors.forename: I
authors.initials: I
authors.lastname: Errabih
authors.forename: H
authors.initials: H
authors.lastname: Ouazzani
authors.forename: A
authors.initials: A
authors.lastname: Sefiani
mesh_terms: D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D003424:Crohn Disease / Q000453:epidemiology / Q000235:genetics*; D005260:Female; D056726:Genetic Association Studies; D005828:Genetics, Population; D006801:Humans; D008297:Male; D008875:Middle Aged; D009018:Morocco / Q000453:epidemiology; D053473:Nod2 Signaling Adaptor Protein / Q000235:genetics*; D010865:Pilot Projects; D011110:Polymorphism, Genetic / Q000502:physiology; D055815:Young Adult
chemical_list: C429018:NOD2 protein, human; D053473:Nod2 Signaling Adaptor Protein