title: Determination of chronic inflammatory states in cancer patients using assay of reactive oxygen species production by neutrophils.
abstract: BACKGROUND
  Although the systemic inflammatory condition can be confirmed in cancer patients, the pathophysiological importance of reactive oxygen species (ROS) produced by neutrophils has not yet been defined.
  
  
  METHODS
  Twenty-one patients with inoperable, chemoresistant and radioresistant cancer were enrolled in this study. At least 4 weeks prior to sampling, the patients were free from antitumor treatments. Control samples were also obtained from a healthy donor (39-year-old male). Peripheral blood samples were set 150 μl each on the 2 ml tube with 50 μl Mebiol Gel, and the production of ROS from neutrophils was detected by luminol-dependent chemiluminescence (LmCL) in a kinetic mode at 30-minute intervals for 2.5 hours with a luminometer at 37°C.
  
  
  RESULTS
  Each point, peak value and sum of values of LmCL in the patient group was statistically higher than those in the healthy donor. There were no differences in LmCL according to performance status (PS), type of cancer, age, or gender in cancer patients.
  
  
  CONCLUSIONS
  Our findings suggested that ROS produced by neutrophils universally reflects the systemic inflammatory condition in cancer patients.
authors.affiliation: Cooperative Major in Advanced Health Science, Graduate School of Collaborative Education Curriculum, Tokyo University of Agriculture and Technology, Waseda University, Tokyo, [corrected] Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ohno
authors.forename: Ryuji
authors.initials: R
authors.lastname: Okuyama
authors.forename: Atsushi
authors.initials: A
authors.lastname: Aruga
authors.forename: Masakazu
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Shigeki
authors.initials: S
authors.lastname: Miura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yoshioka
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Mori
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Suzuki
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D007249:Inflammation / Q000097:blood* / Q000473:pathology; D008163:Luminescent Measurements / Q000379:methods; D008165:Luminol; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000097:blood* / Q000473:pathology; D009504:Neutrophils / Q000378:metabolism* / Q000473:pathology; D009195:Peroxidase / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000097:blood*
chemical_list: D017382:Reactive Oxygen Species; D008165:Luminol; D009195:Peroxidase