title: Complete sequence of a viral nervous necrosis virus (NNV) isolated from red-spotted grouper (Epinephelus akaara) in China.
abstract: A nodavirus isolated from red-spotted grouper (Epinephelus akaara) larvae in China has been subjected to genome analysis. The full-length genome sequences of RNA1 and RNA2 were determined, and the 5'-non-coding region (NCR) and 3'NCR sequences were determined by 5' rapid amplification of cDNA ends (RACE) and 3'RACE. RNA1 is 3,103 nt in length and contains a 982-amino-acid open reading frame (ORF) encoding protein A with a calculated molecular mass of 110.74 kDa. RNA2 is 1,433 nt long and contains a 338-amino-acid major ORF encoding coat protein with a calculated molecular mass of 37.059 kDa. Multiple alignment and phylogenetic analysis clearly supported including this virus in the species Redspotted grouper nervous necrosis virus, genus Betanodavirus, family Nodaviridae.
authors.affiliation: Shenzhen Exit & Entry Inspection and Quarantine Bureau, 1011 Fuqiang Road, Futian, Shenzhen, 518045, People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Yong
authors.initials: Y
authors.lastname: Teng
authors.forename: Xiaocong
authors.initials: X
authors.lastname: Zheng
authors.forename: Yurong
authors.initials: Y
authors.lastname: Wu
authors.forename: Xiayang
authors.initials: X
authors.lastname: Xie
authors.forename: Junqiang
authors.initials: J
authors.lastname: He
authors.forename: Yiyou
authors.initials: Y
authors.lastname: Ye
authors.forename: Zhixin
authors.initials: Z
authors.lastname: Wu
mesh_terms: D000818:Animals; D001492:Bass; D002681:China; D016000:Cluster Analysis; D005393:Fish Diseases / Q000821:virology*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D008970:Molecular Weight; D030381:Nodaviridae / Q000235:genetics* / Q000302:isolation & purification; D016366:Open Reading Frames; D010802:Phylogeny; D012327:RNA Virus Infections / Q000662:veterinary* / Q000821:virology; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins