title: Cloning and sequencing of inulinase and β-fructofuranosidase genes of a deep-sea Microbulbifer species and properties of recombinant enzymes.
abstract: An inulinase-producing Microbulbifer sp. strain, JAM-3301, was isolated from a deep-sea sediment. An inulin operon that contained three open reading frames was cloned and sequenced. Two of the three genes were expressed. One product was an endo-inulinase, and the other was a β-fructofuranosidase. Both enzymes worked together to effectively degrade inulin.
authors.affiliation: Institute of Biogeosciences, Japan Agency for Marine-Earth Science and Technology, Yokosuka, Kanagawa, Japan. kobayashit@jamstec.go.jp
authors.forename: Tohru
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Kohsuke
authors.initials: K
authors.lastname: Uchimura
authors.forename: Shigeru
authors.initials: S
authors.lastname: Deguchi
authors.forename: Koki
authors.initials: K
authors.lastname: Horikoshi
mesh_terms: D034241:Alteromonadaceae / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D003001:Cloning, Molecular; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D007444:Inulin / Q000378:metabolism; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D009876:Operon; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013379:Substrate Specificity; D043324:beta-Fructofuranosidase / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D012336:RNA, Ribosomal, 16S; D011994:Recombinant Proteins; D007444:Inulin; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase; C023086:inulinase