title: Chemometrics optimization of six antihistamines separations by capillary electrophoresis with electrochemiluminescence detection.
abstract: This work expanded the knowledge of the use of chemometric experimental design in optimizing of six antihistamines separations by capillary electrophoresis with electrochemiluminescence detection. Specially, central composite design was employed for optimizing the three critical electrophoretic variables (Tris-H(3)PO(4) buffer concentration, buffer pH value and separation voltage) using the chromatography resolution statistic function (CRS function) as the response variable. The optimum conditions were established from empirical model: 24.2mM Tris-H(3)PO(4) buffer (pH 2.7) with separation voltage of 15.9 kV. Applying theses conditions, the six antihistamines (carbinoxamine, chlorpheniramine, cyproheptadine, doxylamine, diphenhydramine and ephedrine) could be simultaneous separated in less than 22 min. Our results indicate that the chemometrics optimization method can greatly simplify the optimization procedure for multi-component analysis. The proposed method was also validated for linearity, repeatability and sensitivity, and was successfully applied to determine these antihistamine drugs in urine.
authors.affiliation: Department of Medicinal Chemistry and Pharmaceutical Analysis, Guangdong Medical College, Dongwan, Guangdong, China.
authors.forename: Derong
authors.initials: D
authors.lastname: Zhu
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.forename: Jinying
authors.initials: J
authors.lastname: Sun
authors.forename: Tianyan
authors.initials: T
authors.lastname: You
mesh_terms: D002021:Buffers; D002744:Chlorpheniramine / Q000652:urine; D003533:Cyproheptadine / Q000652:urine; D004155:Diphenhydramine / Q000652:urine; D004319:Doxylamine / Q000652:urine; D004563:Electrochemistry; D019075:Electrophoresis, Capillary; D004809:Ephedrine / Q000652:urine; D006633:Histamine Antagonists / Q000652:urine*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008163:Luminescent Measurements; D011725:Pyridines / Q000652:urine; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D002021:Buffers; D006633:Histamine Antagonists; D011725:Pyridines; D003533:Cyproheptadine; D002744:Chlorpheniramine; D004155:Diphenhydramine; D004319:Doxylamine; C004649:carbinoxamine; D004809:Ephedrine