title: A novel method for detecting antigen-specific human regulatory T cells.
abstract: Antigenic epitopes recognized by FoxP3(+) regulatory T cells (Treg) are poorly defined, largely due to a lack of assays for determining Treg specificity. We have developed a novel approach for detecting human Treg specific to peptide antigen, utilizing down-regulation of surface CD3 as a read-out of antigen recognition. Culture conditions and re-stimulation time have been optimized, allowing the detection of even very rare Treg, such as those specific to tumor antigens.
authors.affiliation: Ludwig Institute for Cancer Research (Melbourne-Austin Branch), Melbourne, Australia.
authors.forename: Lisa M
authors.initials: LM
authors.lastname: Ebert
authors.forename: Sarah E
authors.initials: SE
authors.lastname: MacRaild
authors.forename: Ian D
authors.initials: ID
authors.lastname: Davis
authors.forename: Jonathan
authors.initials: J
authors.lastname: Cebon
authors.forename: Weisan
authors.initials: W
authors.lastname: Chen
mesh_terms: D017951:Antigen Presentation / Q000276:immunology; D017252:CD3 Complex / Q000235:genetics / Q000276:immunology; D015536:Down-Regulation / Q000276:immunology; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D005434:Flow Cytometry / Q000379:methods*; D051858:Forkhead Transcription Factors / Q000276:immunology; D006801:Humans; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
chemical_list: D017252:CD3 Complex; D018984:Epitopes, T-Lymphocyte; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors