title: Vibrio alfacsensis sp. nov., isolated from marine organisms.
abstract: Five strains (CAIM 1831(T), CAIM 1832, CAIM 1833, CAIM 1834 and CAIM 1836) were isolated from cultured sole (Solea senegalensis) in two regions of Spain, two strains (CAIM 404 and CAIM 1294) from wild-caught spotted rose snapper (Lutjanus guttatus) in Mexico, and one strain (CAIM 1835) from corals in Brazil. The 16S rRNA gene sequences of the novel isolates showed similarity to Vibrio ponticus (98.2-98.3%, GenBank accession no. AJ630103) and to a lesser degree to Vibrio furnissii (97.2-97.3%, X76336) and to Vibrio fluvialis (96.9-97.1%, X74703). Multilocus sequence analysis clustered these strains closely together and clearly separated them from phylogenetically related species of the genus Vibrio. Genomic fingerprinting by rep-PCR clustered the novel strains according to their geographical origin. Phenotypic analyses showed a large variation among the new strains, but many tests enabled them to be differentiated from other species of the genus Vibrio. The mean ΔT(m) values between the strains analysed here and closely related type strains were above 6.79 °C. The values between the novel isolates were below 2.35 °C, well outside the limit suggested for the delineation of a bacterial species. The phenotypic and genotypic data presented here clearly place these new strains as a coherent group within the genus Vibrio, for which we propose the name Vibrio alfacsensis sp. nov. with CAIM 1831(T) ( = DSM 24595(T) = S277(T)) as the type strain.
authors.affiliation: CIAD, A.C. Mazatlán Unit for Aquaculture and Environmental Management, AP. 711 Mazatlán, CP 82000 Sinaloa, México.
authors.forename: Bruno
authors.initials: B
authors.lastname: Gomez-Gil
authors.affiliation: Institut de Reçerca i Tecnologia Agroalimentaries, Centre d'Aquicultura, Sant Carles de la Rapita, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Roque
authors.affiliation: Universidade Federal de Rio de Janeiro, Ilh Cidade Universitária, Rio de Janeiro 21941-970, Brazil.
authors.forename: Luciane
authors.initials: L
authors.lastname: Chimetto
authors.affiliation: Universidade Federal de Rio de Janeiro, Ilh Cidade Universitária, Rio de Janeiro 21941-970, Brazil.
authors.forename: Ana Paula B
authors.initials: APB
authors.lastname: Moreira
authors.affiliation: DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstr. 7B, 38124 Braunschweig, Germany.
authors.forename: Elke
authors.initials: E
authors.lastname: Lang
authors.affiliation: Universidade Federal de Rio de Janeiro, Ilh Cidade Universitária, Rio de Janeiro 21941-970, Brazil.
authors.forename: Fabiano L
authors.initials: FL
authors.lastname: Thompson
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000382:microbiology; D015373:Bacterial Typing Techniques; D001938:Brazil; D004269:DNA, Bacterial / Q000235:genetics; D005412:Flatfishes / Q000382:microbiology; D008800:Mexico; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D010473:Perciformes / Q000382:microbiology; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013030:Spain; D014733:Vibrio / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S