title: Molecularly imprinted cryogel for L-glutamic acid separation.
abstract: A molecular recognition based L-glutamic acid (L-GLU) imprinted cryogel was prepared for L-GLU separation via chromatographic applications. The novel functional monomer N-methacryloyl-(L)-glutamic acid-Fe(3+) (MAGA-Fe(3+) ) was synthesized to be complex with L-GLU. The L-GLU imprinted cryogel was prepared by free radical polymerization under semifrozen conditions in the presence of a monomer-template complex MAGA-Fe(3+) -L-GLU. The binding mechanism of MAGA-Fe(3+) and L-GLU was characterized by Fourier transform infrared (FTIR) spectroscopy in detail. FTIR analyses on the synthesized MAGA-Fe(3+) -GLU complex reveals bridging bidentate and monodentate binding modes of Fe(3+) in complex with the carboxylate groups of the glutamate residues. The template L-GLU could be reversibly detached from the cryogel to form the template cavities using a 100 mM solution of HNO(3) . The amount of adsorbed L-GLU was detected using the phenyl isothiocyanate method. The L-GLU adsorption capacity of the cryogel decreased drastically from 11.3 to 6.4 μmol g(-1) as the flow rate increased from 0.5 to 4.0 mL min(-1) . The adsorption onto the L-GLU imprinted cryogel was highly pH dependent due to electrostatic interaction between the L-GLU and MAGA-Fe(3+) . The PHEMAGA-Fe(3+) -GLU cryogel exhibited high selectivity to the corresponding guest amino acids (i.e., D-GLU, L-ASN, L-GLN, L-, and D-ASP). Finally, the L-GLU imprinted cryogel was recovered and reused many times, with no significant decrease in their adsorption capacities.
authors.affiliation: Dept. of Chemistry, Biochemistry Div., Hacettepe University, 06532 Beytepe, Ankara, Turkey.
authors.forename: Cemil
authors.initials: C
authors.lastname: Aydoğan
authors.forename: Muge
authors.initials: M
authors.lastname: Andaç
authors.forename: Engin
authors.initials: E
authors.lastname: Bayram
authors.forename: Rıdvan
authors.initials: R
authors.lastname: Say
authors.forename: Adil
authors.initials: A
authors.lastname: Denizli
mesh_terms: D000327:Adsorption; D002845:Chromatography / Q000295:instrumentation*; D059985:Cryogels / Q000138:chemical synthesis / Q000737:chemistry*; D018698:Glutamic Acid / Q000737:chemistry / Q000302:isolation & purification*; D007700:Kinetics; D054802:Molecular Imprinting
chemical_list: D059985:Cryogels; D018698:Glutamic Acid