title: Evaluation of plasmatic MMP-8, MMP-9, TIMP-1 and MPO levels in obese and lean women.
abstract: OBJECTIVE
  To compare the plasma concentrations of matrix metalloproteinase (MMP)-9, tissue inhibitor of MMP (TIMP)-1, MMP-8, and myeloperoxidase (MPO) for obese and lean women.
  
  
  METHODS
  We recruited 30 lean and 36 obese women without comorbidities. The MMP-9, TIMP-1, and MMP-8 levels were measured using enzyme-linked immunosorbent assay (ELISA). MPO activity was assessed by a colorimetric assay.
  
  
  RESULTS
  Obese women had higher MMP-9 levels and MMP-9:TIMP-1 ratios than lean women. Conversely, the MMP-8 levels and MMP-8:TIMP-1 ratios in the obese women were significantly lower than those in the lean women despite neutrophil activation, which was assessed by MPO activity.
  
  
  CONCLUSIONS
  We observed that MMP-9 and MMP-8 had distinct profiles, which suggested that these 2 enzymes play different roles in obesity.
authors.affiliation: Núcleo de Pós-Graduação e Pesquisa, Santa Casa de Belo Horizonte, Rua Domingos Vieira 590, Belo Horizonte, MG, Brazil.
authors.forename: Vanessa L
authors.initials: VL
authors.lastname: Andrade
authors.forename: Eveline
authors.initials: E
authors.lastname: Petruceli
authors.forename: Vanessa A
authors.initials: VA
authors.lastname: Belo
authors.forename: Claudia M
authors.initials: CM
authors.lastname: Andrade-Fernandes
authors.forename: Cristiane V
authors.initials: CV
authors.lastname: Caetano Russi
authors.forename: Adriana Ap
authors.initials: AA
authors.lastname: Bosco
authors.forename: José Eduardo
authors.initials: JE
authors.lastname: Tanus-Santos
authors.forename: Valeria Cristina
authors.initials: VC
authors.lastname: Sandrim
mesh_terms: D000328:Adult; D005260:Female; D006801:Humans; D020784:Matrix Metalloproteinase 8 / Q000097:blood*; D020780:Matrix Metalloproteinase 9 / Q000097:blood*; D008875:Middle Aged; D009765:Obesity / Q000097:blood*; D009195:Peroxidase / Q000097:blood*; D013851:Thinness / Q000097:blood*; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000097:blood*
chemical_list: C560822:TIMP1 protein, human; D019715:Tissue Inhibitor of Metalloproteinase-1; D009195:Peroxidase; C488762:MMP8 protein, human; D020784:Matrix Metalloproteinase 8; D020780:Matrix Metalloproteinase 9