title: Expression of fascin protein and mRNA in the KB carcinoma cell line following treatment with doxorubicin.
abstract: BACKGROUND
  Fascin is an actin-bundling protein that has been shown to be overexpressed in various carcinomas, but the effect of doxorubicin on its protein and mRNA has not been investigated.
  
  
  OBJECTIVE
  The aim of the present study is to evaluate any changes that may be induced by doxorubicin on the fascin molecule in a cancer cell line.
  
  
  METHODS
  Experimental study.
  
  
  METHODS
  The expression of fascin protein and mRNA in the human KB carcinoma cell line was evaluated by qRT-PCR, and immunocytochemistry following treatment with doxorubicin. Apoptosis was also analyzed using flow cytometry.
  
  
  METHODS
  Group comparisons were performed using one-way analysis of variance (ANOVA) followed by Tukey's post hoc test to determine differences between groups (P < 0.05).
  
  
  RESULTS
  Treatment with doxorubicin promoted a significant increase of fascin mRNA and decrease of fascin protein (P <0.05). Doxorubicin also induced apoptosis in the KB cell line.
  
  
  CONCLUSIONS
  Regarding the changes in the actin-bundling protein, fascin, which was found following treatment with doxorubicin, it can be hypothesized that this drug may possess antimigratory characteristics by influencing fascin. Further studies are suggested to clarify this theory.
authors.affiliation: Dental Research Center, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mojgan
authors.initials: M
authors.lastname: Alaeddini
authors.forename: Shamileh
authors.initials: S
authors.lastname: Fouladdel
authors.forename: Shahroo
authors.initials: S
authors.lastname: Etemad-Moghadam
authors.forename: Shahroo
authors.initials: S
authors.lastname: Etemad-Moghaddam
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Azizi
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002352:Carrier Proteins / Q000032:analysis* / Q000235:genetics; D004317:Doxorubicin / Q000494:pharmacology*; D006801:Humans; D007624:KB Cells; D019394:Ki-67 Antigen / Q000235:genetics; D008840:Microfilament Proteins / Q000032:analysis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D000903:Antibiotics, Antineoplastic; D002352:Carrier Proteins; D019394:Ki-67 Antigen; D008840:Microfilament Proteins; D012333:RNA, Messenger; C020324:fascin; D004317:Doxorubicin