title: Blending Baeyer-Villiger monooxygenases: using a robust BVMO as a scaffold for creating chimeric enzymes with novel catalytic properties.
abstract: The thermostable Baeyer-Villiger monooxygenase (BVMO) phenylacetone monooxygenase (PAMO) is used as a scaffold to introduce novel selectivities from other BVMOs or the metagenome by structure-inspired subdomain exchanges. This yields biocatalysts with new preferences in the oxidation of sulfides and the Baeyer-Villiger oxidation of ketones, all while maintaining most of the original thermostability.
authors.affiliation: Laboratory of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands.
authors.forename: Hugo L
authors.initials: HL
authors.lastname: van Beek
authors.forename: Gonzalo
authors.initials: G
authors.lastname: de Gonzalo
authors.forename: Marco W
authors.initials: MW
authors.lastname: Fraaije
mesh_terms: D000096:Acetone / Q000031:analogs & derivatives / Q000378:metabolism; D055162:Biocatalysis; D007700:Kinetics; D054892:Metagenome; D006899:Mixed Function Oxygenases / Q000737:chemistry* / Q000235:genetics; D008958:Models, Molecular; D009247:NADH, NADPH Oxidoreductases / Q000737:chemistry / Q000235:genetics; D010084:Oxidation-Reduction; D010105:Oxygenases / Q000737:chemistry / Q000235:genetics; D015202:Protein Engineering; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000235:genetics; D013237:Stereoisomerism; D013379:Substrate Specificity
chemical_list: D011993:Recombinant Fusion Proteins; D000096:Acetone; D006899:Mixed Function Oxygenases; D010105:Oxygenases; C019961:cyclohexanone oxygenase; D009247:NADH, NADPH Oxidoreductases; C429539:NAD phosphite oxidoreductase; C008863:1-phenyl-2-propanone