title: Detection of nitros(yl)ated metabolites of nitric oxide (NO) in vivo by gas-phase chemiluminescence assay.
abstract: There are several methods utilized for detection of nitric oxide (NO) and its various metabolites in biological systems. These include spectrophotometric analysis, high performance liquid chromatography, mass spectrophotometry, electro-paramagnetic resonance (EPR), and gas-phase chemiluminscence. Of these, the gas-phase chemiluminescence technique is perhaps the most sensitive, with a limit of detection in the femtomole range. Furthermore, as there are a number of commercially available NO analyzers, it is possible to directly compare results from different laboratories using this technique. Described in this unit is a group-specific derivatization approach with gas-phase chemiluminscence detection to profile NO metabolites in vivo.
authors.affiliation: NitroMed, Inc., Lexington, Massachusetts, USA.
authors.forename: Vijay
authors.initials: V
authors.lastname: Dhawan
authors.forename: David
authors.initials: D
authors.lastname: Janero
authors.forename: James
authors.initials: J
authors.lastname: Ellis
mesh_terms: D000818:Animals; D003625:Data Collection; D005740:Gases / Q000032:analysis*; D049449:Luminescence; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D009569:Nitric Oxide / Q000378:metabolism*; D051381:Rats; D013048:Specimen Handling / Q000379:methods
chemical_list: D005740:Gases; D009569:Nitric Oxide