title: [In vitro experimental study of rat cardiomyocyte injury with targeting of perfluorocarbon lipid particles].
abstract: The present study was to investigate in vitro the rat cardiomyocyte injury with targeting of home-made perfluorocarbon lipid particles with avidin-biotin interaction. Neonatal rat cardiomyocytes were cultured in vitro and divided into two groups: TNF-alpha activated group and non-activated group. Those in the TNF-alpha activated group were exposed to 200 ng/ml TNF-alpha solution for 6 hours and then cardiomyocytes in both groups were pretargeted with biotinylated ICAM-1 monoclonal antibodies, and were exposed to streptavidin, and then to homemade green fluorescently-labeled biotinylated perfluorocarbon lipid particles. Cardiomyocytes nucleus stained with Hoechst. The results were detected with fluorescence microscope. As a result, in TNF-alpha activated group, around blue fluorescent cardiomyocytes nucleus, a great amount of green fluorescent particles were found, while there were few green fluorescent particles in non-TNF activated group. It has been shown that ICAM-1 is expressed in the surface of cardiomyocytes when they are stimulated by TNF-alpha. Perfluorocarbon lipid particles associated with ICAM-1 monoclonal antibodies can be targeted to injured cardiomyocytes by avidin-biotin interaction.
authors.affiliation: Department of Cardiology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Baiyong
authors.initials: B
authors.lastname: He
authors.forename: Zhaohuan
authors.initials: Z
authors.lastname: Li
authors.forename: Hong
authors.initials: H
authors.lastname: Tang
authors.forename: Guohua
authors.initials: G
authors.lastname: Li
authors.forename: Song
authors.initials: S
authors.lastname: Chen
authors.forename: Lian
authors.initials: L
authors.lastname: Wang
authors.forename: Haibo
authors.initials: H
authors.lastname: Song
authors.forename: Hua
authors.initials: H
authors.lastname: Fang
authors.forename: Jun
authors.initials: J
authors.lastname: Zeng
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000911:Antibodies, Monoclonal / Q000378:metabolism; D002478:Cells, Cultured; D003287:Contrast Media; D005260:Female; D005466:Fluorocarbons / Q000276:immunology / Q000378:metabolism*; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D008055:Lipids / Q000737:chemistry; D008297:Male; D008863:Microspheres; D032383:Myocytes, Cardiac / Q000378:metabolism / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D014463:Ultrasonography
chemical_list: D000911:Antibodies, Monoclonal; D003287:Contrast Media; D005466:Fluorocarbons; D008055:Lipids; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1