title: Escherichia coli of human and avian origin: detection of clonal groups associated with fluoroquinolone and multidrug resistance in Italy.
abstract: OBJECTIVE
  Poultry have been suggested as a reservoir for fluoroquinolone-resistant extraintestinal pathogenic Escherichia coli (ExPEC). Our aim was to investigate whether genotypes associated with ciprofloxacin and multidrug resistance were shared among human and avian E. coli.
  
  
  METHODS
  We compared 277 human ExPEC isolates from urinary tract infection (UTI) and sepsis (142 susceptible and 135 ciprofloxacin resistant) and 101 avian isolates (68 susceptible and 33 ciprofloxacin resistant) by antimicrobial resistance phenotype, phylogenetic group and multilocus sequence type (ST).
  
  
  RESULTS
  Most ciprofloxacin-resistant isolates from both human and avian sources were multidrug resistant. Human and avian isolates strongly differed in phylogenetic group assignment (B2 and A predominated among human and avian isolates, respectively), but a shift towards group A associated with ciprofloxacin resistance was observed among human isolates (8/100, 8.0% versus 17/87, 19.5%, P =0.021 for UTI and 5/42, 11.9% versus 15/48, 31.3%, P = 0.028 for sepsis). Heterogeneity of ST clones was observed, with ST131 strongly predominant in human ciprofloxacin-resistant strains (58/135, 43.0%), but not in avian strains. However, two major ST clonal complexes (CCs; CC10 and CC23, both belonging to group A) associated with ciprofloxacin resistance and multiresistance were shared by human and avian isolates.
  
  
  CONCLUSIONS
  The major human and avian E. coli ST clones associated with multidrug resistance were identified. A subset of ST clones belonging to CC10 and CC23 poses a potential zoonotic risk.
authors.affiliation: Department of Infectious, Parasitic and Immune-mediated Diseases,  Istituto Superiore di Sanità, Rome,  Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Giufrè
authors.forename: Caterina
authors.initials: C
authors.lastname: Graziani
authors.forename: Marisa
authors.initials: M
authors.lastname: Accogli
authors.forename: Ida
authors.initials: I
authors.lastname: Luzzi
authors.forename: Luca
authors.initials: L
authors.lastname: Busani
authors.forename: Marina
authors.initials: M
authors.lastname: Cerquetti
mesh_terms: D000293:Adolescent; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002645:Chickens; D002648:Child; D002675:Child, Preschool; D016000:Cluster Analysis; D018432:Drug Resistance, Multiple; D024901:Drug Resistance, Multiple, Bacterial; D004926:Escherichia coli / Q000187:drug effects* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology* / Q000662:veterinary*; D005260:Female; D024841:Fluoroquinolones / Q000494:pharmacology*; D005838:Genotype; D006801:Humans; D007223:Infant; D007558:Italy; D008297:Male; D058885:Multilocus Sequence Typing; D011201:Poultry Diseases / Q000382:microbiology*; D018805:Sepsis / Q000382:microbiology; D014422:Turkeys; D014552:Urinary Tract Infections / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones