title: Molecular analysis of imipenem-resistant Acinetobacter baumannii isolated from US service members wounded in Iraq, 2003-2008.
abstract: Global dissemination of imipenem-resistant (IR) clones of Acinetobacter baumannii - A. calcoaceticus complex (ABC) have been frequently reported but the molecular epidemiological features of IR-ABC in military treatment facilities (MTFs) have not been described. We characterized 46 IR-ABC strains from a dataset of 298 ABC isolates collected from US service members hospitalized in different US MTFs domestically and overseas during 2003-2008. All IR strains carried the bla(OXA-51) gene and 40 also carried bla(OXA-23) on plasmids and/or chromosome; one carried bla(OXA-58) and four contained ISAbal located upstream of bla(OXA-51). Strains tended to cluster by pulsed-field gel electrophoresis profiles in time and location. Strains from two major clusters were identified as international clone I by multilocus sequence typing.
authors.affiliation: Division of Bacterial and Rickettsial Diseases, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA.
authors.forename: X-Z
authors.initials: XZ
authors.lastname: Huang
authors.forename: M A
authors.initials: MA
authors.lastname: Chahine
authors.forename: J G
authors.initials: JG
authors.lastname: Frye
authors.forename: D M
authors.initials: DM
authors.lastname: Cash
authors.forename: E P
authors.initials: EP
authors.lastname: Lesho
authors.forename: D W
authors.initials: DW
authors.lastname: Craft
authors.forename: L E
authors.initials: LE
authors.lastname: Lindler
authors.forename: M P
authors.initials: MP
authors.lastname: Nikolich
mesh_terms: D000151:Acinetobacter Infections / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology*; D040981:Acinetobacter baumannii / Q000145:classification / Q000235:genetics*; D016954:Acinetobacter calcoaceticus / Q000145:classification / Q000235:genetics; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D016521:Electrophoresis, Gel, Pulsed-Field; D005858:Germany / Q000453:epidemiology; D006801:Humans; D015378:Imipenem / Q000627:therapeutic use*; D054542:Iraq War, 2003-2011; D008826:Microbial Sensitivity Tests; D008889:Military Personnel; D017720:Molecular Epidemiology; D058885:Multilocus Sequence Typing; D058974:Phylogeography; D014481:United States / Q000453:epidemiology; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D015378:Imipenem; C466679:beta-lactamase OXA-23; D001618:beta-Lactamases; C577374:beta-lactamase OXA-143, Acinetobacter baumannii; C410345:beta-lactamase OXA-24; C488541:beta-lactamase OXA-40, Acinetobacter baumannii; C577373:beta-lactamase OXA-51, Acinetobacter baumannii; C534889:beta-lactamase OXA-58, Acinetobacter baumannii