title: Evaluation of four antiseptics using a novel murine norovirus.
abstract: We isolated a novel murine norovirus (MNV), MT30-2 strain, from feces of conventional mice in Japan to evaluate the virucidal activity of four antiseptics. The MNV MT30-2 strain was inactivated by as little as 0.2% (w/v) povidone-iodine (PVP-I) and 0.1% (w/v) sodium hypochlorite (NaOCl) treatment as determined by a novel plaque assay. Importantly, PVP-I reduced the MNV titer by 4 log(10) within 15 s of exposure. The other two antiseptics, benzethonium chloride (BEC) and chlorhexidine gluconate (CHG), did not reduce the MNV titer even when treatment lasted for 60 s. When the virus titer was reduced by PVP-I or NaOCl treatment, the amount of MNV RNA was not reduced, indicating that the presence of viral RNA was not related to the virucidal activity of the antiseptics. PVP-I and NaOCl will be useful in controlling the spread of MNV, which is a common problem in mice colonies. In this study, we isolated a novel MNV and newly revealed that two antiseptics (PVP-I and NaOCl) were able to inactivate MNV at low concentrations and in a short contact time.
authors.affiliation: Pharmaceutical Research Center, Meiji Seika Pharma Co., Ltd.
authors.forename: Takashi
authors.initials: T
authors.lastname: Matsuhira
authors.forename: Chizuko
authors.initials: C
authors.lastname: Kaji
authors.forename: Shoichi
authors.initials: S
authors.lastname: Murakami
authors.forename: Kazunori
authors.initials: K
authors.lastname: Maebashi
authors.forename: Tomoichiro
authors.initials: T
authors.lastname: Oka
authors.forename: Naokazu
authors.initials: N
authors.lastname: Takeda
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Katayama
mesh_terms: D000818:Animals; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D017250:Caliciviridae Infections / Q000517:prevention & control / Q000662:veterinary*; D002460:Cell Line; D005243:Feces / Q000821:virology; D007564:Japan; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D029322:Norovirus / Q000187:drug effects* / Q000302:isolation & purification*; D010802:Phylogeny; D011206:Povidone-Iodine / Q000494:pharmacology*; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017423:Sequence Analysis, RNA; D012973:Sodium Hypochlorite / Q000494:pharmacology*
chemical_list: D000891:Anti-Infective Agents, Local; D012367:RNA, Viral; D011206:Povidone-Iodine; D012973:Sodium Hypochlorite