title: Hyperoxidized peroxiredoxins in peripheral blood mononuclear cells of asthma patients is associated with asthma severity.
abstract: OBJECTIVE
  Oxidative stress is involved in the pathogenesis of asthma, and peroxiredoxins (PRDX) may be critical in controlling intracellular oxidative stress. The aim of this study was to evaluate expressions of PRDX and their hyperoxidized forms in asthmatic individuals.
  
  
  METHODS
  The levels of expression of PRDX1, PRDX2, PRDX3, and PRDX6 and their hyperoxidized forms (PRDX-SO(3)) were measured in PBMCs from asthma patients and control subjects. In addition, cells from these subjects were treated with hydrogen peroxide (H(2)O(2)) and their intracellular concentrations of reactive oxygen species (ROS) were measured.
  
  
  RESULTS
  The ratios of hyperoxidized to total PRDX (PRDX-SO(3/)PRDX) in PBMCs were significantly higher in asthma patients than in normal subjects and were correlated with disease severity, with the highest ratio seen in patients with severe asthma. Furthermore, H(2)O(2) treatment of PBMCs, particularly lymphocytes, increased intracellular ROS concentrations with greater and more persistent increases observed in cells from asthmatic than from control subjects.
  
  
  CONCLUSIONS
  Hyperoxidation of PRDX may serve as a biomarker of asthma severity and may predict enhanced susceptibility to oxidative stress load in PBMCs of asthmatics.
authors.affiliation: Department of Allergy and Clinical Immunology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea.
authors.forename: Hyouk-Soo
authors.initials: HS
authors.lastname: Kwon
authors.forename: Yun-Jeong
authors.initials: YJ
authors.lastname: Bae
authors.forename: Keun-Ai
authors.initials: KA
authors.lastname: Moon
authors.forename: Yoon Su
authors.initials: YS
authors.lastname: Lee
authors.forename: Taehoon
authors.initials: T
authors.lastname: Lee
authors.forename: Ki-Young
authors.initials: KY
authors.lastname: Lee
authors.forename: Tae-Bum
authors.initials: TB
authors.lastname: Kim
authors.forename: Chan-Sun
authors.initials: CS
authors.lastname: Park
authors.forename: Hee-Bom
authors.initials: HB
authors.lastname: Moon
authors.forename: You Sook
authors.initials: YS
authors.lastname: Cho
mesh_terms: D000328:Adult; D001249:Asthma / Q000097:blood* / Q000503:physiopathology*; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D016877:Oxidants / Q000378:metabolism; D018384:Oxidative Stress; D054464:Peroxiredoxins / Q000097:blood* / Q000737:chemistry; D020033:Protein Isoforms / Q000097:blood* / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D016877:Oxidants; D020033:Protein Isoforms; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D054464:Peroxiredoxins