title: Development of EST-SSR markers for Elaeocarpus photiniifolia (Elaeocarpaceae), an endemic taxon of the Bonin Islands.
abstract: OBJECTIVE
  Expressed sequence tag (EST)-derived microsatellite markers were developed for Elaeocarpus photiniifolia, an endemic taxon of the Bonin Islands.
  
  
  RESULTS
  Initially, a complementary DNA (cDNA) library was constructed by de novo pyrosequencing of total RNA extracted from a seedling. A total of 267 primer pairs were designed from the library. Of the 48 tested loci, 25 loci were polymorphic among 41 individuals representing the entire geographical range of the species, with the number of alleles per locus and expected heterozygosity ranging from two to 14 and 0.09 to 0.86, respectively. Most loci were transferable to a related species, E. sylvestris.
  
  
  CONCLUSIONS
  The developed markers will be useful for evaluating the genetic structure of E. photiniifolia.
authors.affiliation: Makino Herbarium, Graduate School of Science and Engineering, Tokyo Metropolitan University, 1-1 Minami-Ohsawa, Hachioji, Tokyo, 192-0397, Japan. sugai-kyoko@ed.tmu.ac.jp
authors.forename: Kyoko
authors.initials: K
authors.lastname: Sugai
authors.forename: Suzuki
authors.initials: S
authors.lastname: Setsuko
authors.forename: Kentaro
authors.initials: K
authors.lastname: Uchiyama
authors.forename: Noriaki
authors.initials: N
authors.lastname: Murakami
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Kato
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yoshimaru
mesh_terms: D000483:Alleles; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D045467:Elaeocarpaceae / Q000235:genetics; D020224:Expressed Sequence Tags; D005787:Gene Frequency; D015723:Gene Library; D056426:Genetic Loci; D006579:Heterozygote; D062312:Islands; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D014170:Transformation, Genetic
chemical_list: D017931:DNA Primers; D018744:DNA, Plant