title: Microsatellite marker development for the threatened orchid Masdevallia solomonii (Orchidaceae).
abstract: OBJECTIVE
  Microsatellite markers for Masdevallia solomonii were developed to serve as a tool in future population genetic studies of this threatened species from the Bolivian Yungas.
  
  
  RESULTS
  Thirteen microsatellite primers were characterized by cloning an intersimple sequence repeat (ISSR) library. From these, 10 loci presented considerable variation in allele number (3-10), expected heterozygosity (0.537-0.865), and polymorphic information content per locus (0.500-0.848).
  
  
  CONCLUSIONS
  The markers obtained for M. solomonii are the first in the genus and subtribe. The observed polymorphism will make it possible to assess genetic diversity and structure of this species and will serve to propose effective conservation actions.
authors.affiliation: Departamento de Ciências Biológicas, Universidade Estadual de Feira de Santana, Av. Transnordestina s.n., 44036-900, Feira de Santana, Bahia, Brazil. macrissroberts@yahoo.es
authors.forename: M Cristina
authors.initials: MC
authors.lastname: López-Roberts
authors.forename: Paulo R M
authors.initials: PR
authors.lastname: Almeida
authors.forename: Eddy J F
authors.initials: EJ
authors.lastname: Oliveira
authors.forename: Cássio
authors.initials: C
authors.lastname: van den Berg
mesh_terms: D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D056727:Endangered Species; D005787:Gene Frequency; D015723:Gene Library; D056426:Genetic Loci; D060005:Genotyping Techniques; D006579:Heterozygote; D018895:Microsatellite Repeats; D029595:Orchidaceae / Q000235:genetics*; D011110:Polymorphism, Genetic
chemical_list: D017931:DNA Primers; D018744:DNA, Plant