title: Detection and molecular characterization of porcine circovirus type 2 (PCV2) from piglets with exudative epidermitis in Uruguay.
abstract: Porcine circovirus type 2 (PCV2) is an economically important emerging pathogen associated with distinct syndromes and diseases in swine, collectively known as porcine circovirus associated diseases (PCVAD). The main purpose of this study was to investigate the presence of PCV2 in piglets affected with exudative epidermitis (EE) in Uruguay. In addition we aimed to analyze the phylogenetic relationships of the isolated strains. In June 2011 an outbreak of EE detected in a small herd was reported. Piglets presented skin lesions compatible with EE and symptoms associated with postweaning multisystemic wasting syndrome (PMWS) were also observed. Sera from affected and healthy animals were tested for the presence of viral DNA. Exclusively, diseased piglets were infected with PCV2. Phylogenetic analysis showed that PCV2 isolates belonged to PCV2b genotype. We report the detection and molecular characterization of PCV2 strains for the first time in Uruguay.
authors.affiliation: Sección Virología, Facultad de Ciencias, Universidad de la República, Iguá 4225, Montevideo 11400, Uruguay.
authors.forename: Natalia
authors.initials: N
authors.lastname: Ramos
authors.forename: Santiago
authors.initials: S
authors.lastname: Mirazo
authors.forename: Gustavo
authors.initials: G
authors.lastname: Castro
authors.forename: Juan
authors.initials: J
authors.lastname: Arbiza
mesh_terms: D000818:Animals; D018173:Circoviridae Infections / Q000453:epidemiology / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004196:Disease Outbreaks / Q000662:veterinary; D004818:Epidermitis, Exudative, of Swine / Q000453:epidemiology / Q000821:virology*; D010802:Phylogeny; D013552:Swine; D014583:Uruguay / Q000453:epidemiology