title: Thermolabile liposomes: a controlled release delivery tool in diagnosis/therapy in experimental pulmonary ɶdema.
abstract: Liposomes, usually assembled from organic/synthetic lipidic compounds, are biocompatible, biodegradable, non-toxic, and do not induce immune response. Due to their structural versatility in terms of size, composition, surface charge, bilayer fluidity and ability to encapsulate drugs regardless of their solubility, liposomes enable the production of a vast number and type of formulations with potential clinical use. They can be administered through several routes of administration (e.g. i.v., i.m., oral, nasal, etc.). The use of liposomes enables the variation and control retention of drugs in biologic fluids, enhancing blood circulation and specific compartments residence. They can be tailored to target specific tissues and cells. They can play a very important role for imaging diagnosis and/or therapy. After an extensive literature review of the subject, we selected a particular area of potential clinical application: pulmonary ɶdema. This clinical entity has a variety of possible etiologies, conducing to two main types of edema: cardiogenic and non-cardiogenic. At the moment a dedicated technique for the early diagnosis/therapy of this pathology is lacking. We propose a new methodology using a specially designed GUV formulation, encapsulating chosen radiotracers labeled with 99mTc. The aim of the work has been successfully achieved in an experimental animal model of cardiogenic pulmonary oedema. Experiments using an animal model of non-cardiogenic pulmonary oedema are in course (simultaneous study with two different drugs), using the same GUV methodology. Preliminary results are very promising.
authors.affiliation: Biophysics/Biomathematics Institute, Medical School, Coimbra University, Azinhaga de Sta Comba, Celas, 3000-548 Coimbra, Portugal. cristina@ibili.uc.pt
authors.forename: A C
authors.initials: AC
authors.lastname: Santos
authors.forename: C M
authors.initials: CM
authors.lastname: Matos
authors.forename: B
authors.initials: B
authors.lastname: Oliveiros
authors.forename: T
authors.initials: T
authors.lastname: Almeida
authors.forename: L
authors.initials: L
authors.lastname: Gano
authors.forename: M
authors.initials: M
authors.lastname: Neves
authors.forename: N
authors.initials: N
authors.lastname: Ferreira
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage*; D003692:Delayed-Action Preparations; D004337:Drug Carriers / Q000008:administration & dosage*; D016503:Drug Delivery Systems; D006801:Humans; D007553:Isotope Labeling / Q000379:methods; D008081:Liposomes / Q000008:administration & dosage* / Q000145:classification; D011654:Pulmonary Edema / Q000188:drug therapy*; D051381:Rats
chemical_list: D000970:Antineoplastic Agents; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D008081:Liposomes