title: Probing the interaction of anti-cancer agent dihydromyricetin with human serum albumin: a typical method study.
abstract: The interaction between dihydromyricetin (DMY) with human serum albumin (HSA) under the physiological conditions was investigated by fluorescence spectroscopy, circular dichroism (CD) spectra and UV-visible absorption spectroscopy. In the mechanism discussion it was proved that the fluorescence quenching of HSA by DMY is a result of the formation of DMY-HSA complex. Binding parameters calculated showed that DMY bind to HSA with the binding affinities of the order 105∼106 L�mol-1. The enthalpy change (ΔH) and entropy change (ΔS) were calculated to be -28.76 kJ�mol-1 and 18.21 J�mol-1�K-1, respectively, which implied that the hydrophobic and hydrogen bonds interactions play predominant roles in the binding process. The binding site of DMY on HSA may be located in hydrophobic cavity of subdomain IIA by the analysis data of fluorescence and synchronous fluorescence spectra. The specific binding distance r (3.37 nm) between donor (Trp-214) and acceptor (DMY) was obtained according to Forster non-radiative resonance energy transfer theory. CD spectral result demonstrates that DMY does not affect the secondary structure of HSA and can maintain protein stabilization. In addition, the effect of some common metal ions (e.g. Zn2+, Cu2+, Co2+, Ni2+, Fe3+) on the binding constant between DMY and HSA was examined.
authors.affiliation: Nantong University, Nantong, 226019, PR China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Chen
authors.forename: Shajun
authors.initials: S
authors.lastname: Zhu
authors.forename: Yapeng
authors.initials: Y
authors.lastname: Lu
authors.forename: Hui
authors.initials: H
authors.lastname: Cao
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhao
authors.forename: Guoqing
authors.initials: G
authors.lastname: Jiang
authors.forename: Li
authors.initials: L
authors.lastname: Zhu
authors.forename: Tianhong
authors.initials: T
authors.lastname: Lu
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry*; D001665:Binding Sites; D001688:Biological Products / Q000737:chemistry*; D044948:Flavonols / Q000737:chemistry*; D006801:Humans; D015394:Molecular Structure; D012709:Serum Albumin / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D001688:Biological Products; D044948:Flavonols; D012709:Serum Albumin; C472036:dihydromyricetin