title: Transcriptome analysis of silver carp (Hypophthalmichthys molitrix) by paired-end RNA sequencing.
abstract: The silver carp (Hypophthalmichthys molitrix) is among the most intensively pond-cultured fish species and is used in the wild to counteract water bloom in China. However, little genomic information is available for this species, especially regarding its ability to grow rapidly in water, even water contaminated with high concentrations of poisonous microcystin. In this study, we performed de novo transcriptome assembly and analysis of the 17.10 million short-read sequences produced by the Illumina paired-end sequencing technology. Using an improved multiple k-mer contig assembly method coupled with further scaffolding, 85,759 sequences were obtained. There were 23,044 sequences annotated with 3423 gene ontology terms for 104 196 term occurrences and the three corresponding organizing principles. A total of 38,200 assembled sequences were involved in 218 predicted Kyoto Encyclopedia of Genes and Genomes metabolic pathways. We also recovered 41 of 44 genes involved in the biosynthesis of glutathione. Of these, five genes were identified as experienced positive selection between silver carp and zebrafish, as determined by the likelihood ratio test. This report is the first annotated review of the silver carp transcriptome. These data will be of interest to researchers investigating the evolution and biological processes of the silver carp. This work also provides an archive for future studies of recent speciation and evolution of Cyprinidae fishes and can be used in comparative studies of other fishes.
authors.affiliation: The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, PR China.
authors.forename: Beide
authors.initials: B
authors.lastname: Fu
authors.forename: Shunping
authors.initials: S
authors.lastname: He
mesh_terms: D000818:Animals; D002347:Carps / Q000235:genetics*; D002681:China; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D030541:Databases, Genetic; D020869:Gene Expression Profiling / Q000379:methods*; D005819:Genetic Markers; D018895:Microsatellite Repeats; D015203:Reproducibility of Results; D017423:Sequence Analysis, RNA / Q000379:methods*
chemical_list: D018076:DNA, Complementary; D005819:Genetic Markers