title: Silk fibroin/poly(vinyl alcohol) photocrosslinked hydrogels for delivery of macromolecular drugs.
abstract: Hydrogels are three-dimensional polymer networks widely used in biomedical applications as drug delivery and tissue engineered scaffolds to effectively repair or replace damaged tissue. In this paper we demonstrate a newly synthesized cytocompatible and drug releasing photo-crosslinked hydrogel based on poly(vinyl alcohol) methacrylate and silk fibroin which possesses tailorable structural and biological properties. The initial silk fibroin content was 0%, 10%, 20%, 30%, 40% and 50% with respect to the weight of poly(vinyl alcohol) methacrylate. The prepared hydrogels were characterized with respect to morphology, crystallinity, stability, swelling, mass loss and cytotoxicity. FITC-dextrans of different molecular weights were chosen as model drugs molecules for release studies from the hydrogels. The hydrogels containing different silk fibroin percentages showed differences in pore size and distribution. X-ray diffraction analysis revealed that amorphous silk fibroin in poly(vinyl alcohol) methacrylate is crystallized to β-sheet secondary structure upon gelation. The sol fraction increased with increasing fibroin concentration in the co-polymer gel (from 18% to 45%), although the hydrogel extracts were non-cytotoxic. Similarly, the addition of silk fibroin increased water uptake by the gels (from 7% to 21%). FITC-dextran release from the hydrogels was dependent on the silk fibroin content and the molecular weight of encapsulated molecules. The study outlines a newer type of photo-crosslinked interpenetrating polymer network hydrogel that possess immense potential in drug delivery applications.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology, Kharagpur 721302, India.
authors.forename: Joydip
authors.initials: J
authors.lastname: Kundu
authors.forename: Laura A
authors.initials: LA
authors.lastname: Poole-Warren
authors.forename: Penny
authors.initials: P
authors.lastname: Martens
authors.forename: Subhas C
authors.initials: SC
authors.lastname: Kundu
mesh_terms: D003432:Cross-Linking Reagents / Q000737:chemistry / Q000528:radiation effects; D003692:Delayed-Action Preparations / Q000737:chemistry* / Q000528:radiation effects; D004058:Diffusion; D005349:Fibroins / Q000737:chemistry* / Q000528:radiation effects; D020100:Hydrogels / Q000737:chemistry* / Q000528:radiation effects; D008027:Light; D046911:Macromolecular Substances / Q000008:administration & dosage / Q000737:chemistry* / Q000528:radiation effects; D008422:Materials Testing; D010777:Photochemistry / Q000379:methods; D011142:Polyvinyl Alcohol / Q000737:chemistry* / Q000528:radiation effects
chemical_list: D003432:Cross-Linking Reagents; D003692:Delayed-Action Preparations; D020100:Hydrogels; D046911:Macromolecular Substances; D011142:Polyvinyl Alcohol; D005349:Fibroins