title: Correlating matrix metalloproteinase-9 sinus secretion levels with tissue biopsy levels.
abstract: BACKGROUND
  Collecting mucosal biopsies is invasive and creates additional inflammation, hampering a better understanding of nasal and sinus disease evolution and response to treatment. We examine whether sinus secretion collection can replace tissue biopsy for protein determination.
  
  
  METHODS
  Prior to surgical intervention for chronic rhinosinusitis (CRS), a piece of gelatin foam was used to collect secretions from the ethmoid mucosa. A tissue biopsy was then taken from the same location. Matrix metalloproteinase-9 (MMP-9) protein levels were measured in each sample.
  
  
  RESULTS
  MMP-9 protein levels in secretions and tissues were significantly correlated (p = 0.0033, r = 0.52, by Pearson correlation).
  
  
  CONCLUSIONS
  Secretion collection can replace tissue biopsy for MMP-9 determinations, reducing morbidity. Furthermore, secretion collection allows sequential sampling from the same location.
authors.affiliation: Department of Surgery, Division of Otolaryngology-Head and Neck Surgery, University of Utah, Salt Lake City, UT 84132, USA. matthew.wilson@hsc.utah.edu
authors.forename: Matt A
authors.initials: MA
authors.lastname: Wilson
authors.forename: Richard R
authors.initials: RR
authors.lastname: Orlandi
authors.forename: Matthew A
authors.initials: MA
authors.lastname: Firpo
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001706:Biopsy; D003430:Cross-Sectional Studies; D004797:Enzyme-Linked Immunosorbent Assay; D015521:Ethmoid Sinusitis / Q000201:enzymology* / Q000473:pathology; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D008875:Middle Aged; D009297:Nasal Mucosa / Q000201:enzymology / Q000473:pathology*; D012130:Respiratory Hypersensitivity / Q000201:enzymology / Q000473:pathology; D012221:Rhinitis, Allergic, Perennial / Q000201:enzymology* / Q000473:pathology; D055815:Young Adult
chemical_list: D020780:Matrix Metalloproteinase 9