title: Antimicrobial activity of Ulopterol isolated from Toddalia asiatica (L.) Lam.: a traditional medicinal plant.
abstract: BACKGROUND
  The leaves of Toddalia asiatica (L.) Lam. (Rutaceae) are widely used in folk medicine in India to treat various ailments like cough, malaria, indigestion, influenza lung diseases and rheumatism, fever, stomach ailments, cholera and diarrhea. In our earlier communication we have reported the antimicrobial study on the various extracts of the leaves and the isolation and identification of Flindersine, a quinolone alkaloid as the major active principle. In the present study, we report the antibacterial and antifungal activities of Ulopterol, a coumarin isolated as another major active antimicrobial principle.
  
  
  METHODS
  The leaves were successively extracted with hexane, chloroform, ethyl acetate, methanol and water. The extracts were studied for their antimicrobial activity against selected bacteria and fungi by using disc-diffusion method. The ethyl acetate extract which was found to possess highest antimicrobial activity was subjected to activity guided fractionation by column chromatography over silica gel. This resulted in the isolation of the coumarin, Ulopetrol, an active principle besides Flindersine which was reported by us earlier. The structure of the compound was elucidated using physical and spectroscopic data. Flindersine and Ulopterol were quantified by HPLC.
  
  
  RESULTS
  Ulopterol showed activity against the bacteria viz. Staphylococcus epidermidis, Enterobacter aerogenes, Shigella flexneri, Klebsiella pneumoniae (ESBL-3967), Escherichia coli (ESBL-3984) and fungi viz. Aspergillus flavus, Candida krusei and Botrytis cinerea. Quantification by HPLC showed the content of Flindersine and Ulopterol to be 0.361% and 0.266% respectively on dry weight basis of the leaves.
  
  
  CONCLUSIONS
  Ethyl acetate extract (successive extraction) contained Ulopterol, a coumarin, besides Flindersine, a quinolone alkaloid, as a major active principle in the antimicrobial studies. This is the first report of the antimicrobial activity of Ulopterol and also its first report from the plant.
authors.affiliation: Division of Ethnopharmacology, Entomology Research Institute, Loyola College, Chennai 600 034, India.
authors.forename: M
authors.initials: M
authors.lastname: Karunai Raj
authors.forename: C
authors.initials: C
authors.lastname: Balachandran
authors.forename: V
authors.initials: V
authors.lastname: Duraipandiyan
authors.forename: P
authors.initials: P
authors.lastname: Agastian
authors.forename: S
authors.initials: S
authors.lastname: Ignacimuthu
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D001231:Aspergillus flavus / Q000187:drug effects; D020171:Botrytis / Q000187:drug effects; D002175:Candida / Q000187:drug effects; D003374:Coumarins / Q000302:isolation & purification / Q000494:pharmacology*; D021902:Enterobacter aerogenes / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D006575:Heterocyclic Compounds, 3-Ring / Q000032:analysis; D007194:India; D007711:Klebsiella pneumoniae / Q000187:drug effects; D008519:Medicine, Traditional; D018515:Plant Leaves; D010946:Plants, Medicinal; D027821:Rutaceae / Q000737:chemistry*; D012763:Shigella flexneri / Q000187:drug effects; D013210:Staphylococcus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D003374:Coumarins; D006575:Heterocyclic Compounds, 3-Ring; C573819:ulopterol; C539535:flindersine