title: Chiral separation of vinpocetine using cyclodextrin-modified micellar electrokinetic chromatography.
abstract: A cyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC) technique has been developed for enantioseparation of vinpocetine using an inexpensive 2-hydroxypropyl-β-CD (HP-β-CD) as the chiral selector (CS). The best chiral separation was achieved using 40 mM HP-β-CD as the CS in 50 mM phosphate buffer (pH 7.0) consisting of 40 mM sodium dodecyl sulfate (SDS) at a separation temperature and separation voltage of 25°C and 25 kV, respectively. To the author's best knowledge, this is the first CD-MEKC study able to successfully separate the four stereoisomer of vinpocetine in separation time of 9.5 min and resolution of 1.04-3.87.
authors.affiliation: Separation Science and Technology Group, Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, Johor, Malaysia. wanaini@kimia.fs.utm.my
authors.forename: Wan Aini
authors.initials: WA
authors.lastname: Wan Ibrahim
authors.forename: Siti Munirah
authors.initials: SM
authors.lastname: Abd Wahib
authors.forename: Dadan
authors.initials: D
authors.lastname: Hermawan
authors.forename: Mohd Marsin
authors.initials: MM
authors.lastname: Sanagi
authors.forename: Hassan Y
authors.initials: HY
authors.lastname: Aboul-Enein
mesh_terms: D002021:Buffers; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D003505:Cyclodextrins / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007267:Injections; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013237:Stereoisomerism; D013997:Time Factors; D014748:Vinca Alkaloids / Q000737:chemistry* / Q000302:isolation & purification*
chemical_list: D002021:Buffers; D003505:Cyclodextrins; D014748:Vinca Alkaloids; D012967:Sodium Dodecyl Sulfate; C013983:vinpocetine