title: Panowamycins A and B, new antitrypanosomal isochromans produced by Streptomyces sp. K07-0010.
abstract: Two new isochromans, panowamycins A and B, were purified by solvent extraction, silica gel and octadecylsilyl silica gel (ODS) column chromatography followed by preparative HPLC, from a culture broth of Streptomyces sp. K07-0010, together with the known compounds NFAT-133, conglobatin, piericidin C series and dinactin. Structures of panowamycins were elucidated as new analogs of NFAT-133 by spectroscopic studies including various NMR experiments. Panowamycins A and B showed moderate antitrypanosomal activity, with IC(50) values of 0.40 and 3.30 μg ml(-1), respectively.
authors.affiliation: Research Center for Tropical Diseases, Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan.
authors.forename: Junko
authors.initials: J
authors.lastname: Hashida
authors.forename: Megumi
authors.initials: M
authors.lastname: Niitsuma
authors.forename: Masato
authors.initials: M
authors.lastname: Iwatsuki
authors.forename: Mihoko
authors.initials: M
authors.lastname: Mori
authors.forename: Aki
authors.initials: A
authors.lastname: Ishiyama
authors.forename: Miyuki
authors.initials: M
authors.lastname: Namatame
authors.forename: Aki
authors.initials: A
authors.lastname: Nishihara-Tsukashima
authors.forename: Atsuko
authors.initials: A
authors.lastname: Matsumoto
authors.forename: Ismet
authors.initials: I
authors.lastname: Ara
authors.forename: Yoko
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Haruki
authors.initials: H
authors.lastname: Yamada
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Otoguro
authors.forename: Kazuro
authors.initials: K
authors.lastname: Shiomi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Omura
mesh_terms: D000981:Antiprotozoal Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001459:Bangladesh; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002839:Chromans / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006801:Humans; D020128:Inhibitory Concentration 50; D008855:Microscopy, Electron, Scanning; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013055:Spectrophotometry, Infrared; D013302:Streptomyces / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D014346:Trypanosoma brucei brucei / Q000187:drug effects
chemical_list: D000981:Antiprotozoal Agents; D002839:Chromans