title: Isolation and characterization of novel microsatellite markers for Avena sativa (Poaceae) (oat).
abstract: OBJECTIVE
  A new set of microsatellite primers was developed for Avena sativa and characterized to assess the level of genetic diversity among cultivars and wild genotypes.
  
  
  RESULTS
  Using an enrichment genomic library, 14 simple sequence repeat markers were identified. The loci of these markers were characterized and found to be polymorphic in size among 48 genotypes of oat from diverse geographical locations. The number of alleles per locus ranged from two to eight, while the observed heterozygosity ranged from 0.031 to 0.75.
  
  
  CONCLUSIONS
  These newly identified microsatellite markers will facilitate genetic diversity studies, fingerprinting, and genetic mapping of oat. Moreover, these new primers for A. sativa will aid future studies of polyploidy and hybridization in other species in this genus.
authors.affiliation: Institute of Crop Science, Chinese Academy of Agricultural Sciences, No.12 Zhongguancun South Street, Beijing 100081, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wu
authors.forename: Zongwen
authors.initials: Z
authors.lastname: Zhang
authors.forename: Lingyun
authors.initials: L
authors.lastname: Chen
authors.forename: Minggao
authors.initials: M
authors.lastname: He
mesh_terms: D018554:Avena / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D030541:Databases, Genetic; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005787:Gene Frequency; D056426:Genetic Loci; D014644:Genetic Variation; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D015698:Genomic Library; D006579:Heterozygote; D018895:Microsatellite Repeats
chemical_list: D017931:DNA Primers; D018744:DNA, Plant