title: Effect of genetic variants related to lipid metabolism as risk factors for cholelithiasis after bariatric surgery in Brazilian population.
abstract: BACKGROUND
  The manifestation of cholelithiasis after bariatric surgery may depend on genetic factors related to lipid metabolism, including apolipoprotein E (APOE) and cholesteryl ester transfer protein (CETP) gene polymorphisms.
  
  
  METHODS
  We investigated the association between APOE HhaI and CETP TaqIB polymorphisms [PCR-RFLP] and occurrence of cholelithiasis over up to 8 months of follow-up after gastroplasty to Roux-en-Y gastric bypass in 220 patients distributed in Group 1 (G1) 114 with cholelithiasis postoperatively and Group 2 (G2) 106 without cholelithiasis, including biochemical and anthropometric profiles analyses.
  
  
  RESULTS
  In our series, the allelic and genotypic distributions of CETP TaqIB and APOE HhaI polymorphisms were similar in both groups (P > 0.05). The subgroup analysis evidenced that 54% of the patients from G1, APOE*4 allele carriers compared with APOE*3/3 carriers, presented altered low-density lipoprotein cholesterol (LDL cholesterol) serum levels (P = 0.022) before bariatric surgery. The B1 allele for CETP was associated to more quickly elevation of HDL cholesterol levels just in individuals without cholelitiasis (P < 0.0001). The multivariate logistic regression analysis demonstrates correlation between APOE*4 allele, higher total cholesterol (TC) serum levels and prediposition to cholelitiasis in preoperative period. However, the presence of postoperative cholelithiasis was not associated with altered lipid profile.
  
  
  CONCLUSIONS
  The CETP TaqIB and APOE HhaI polymorphisms do not seem to have association with gallstones in the late postoperative bariatric surgery, considering that these genetic variants do not differ subgroups of patients who are eligible to routine prophylactic cholecystectomy, at least in Brazilian population.
authors.affiliation: Department of Molecular Biology, Center for Biochemistry and Molecular Biology Research, São José do Rio Preto Medical School, 15090000, Av. Brigadeiro Faria Lima 5416, Vila São Pedro, São José do Rio Preto, São Paulo, Brazil. marcelapinhel@yahoo.com.br
authors.forename: Sidney
authors.initials: S
authors.lastname: Pinheiro-Júnior
authors.forename: Marcela A S
authors.initials: MA
authors.lastname: Pinhel
authors.forename: Marcelo A
authors.initials: MA
authors.lastname: Nakazone
authors.forename: Anielli
authors.initials: A
authors.lastname: Pinheiro
authors.forename: Gisele F S
authors.initials: GF
authors.lastname: Amorim
authors.forename: Greiciane M S
authors.initials: GM
authors.lastname: Florim
authors.forename: Camila M
authors.initials: CM
authors.lastname: Mazeti
authors.forename: Michele L
authors.initials: ML
authors.lastname: Gregório
authors.forename: Marina G
authors.initials: MG
authors.lastname: Moschetta
authors.forename: Gilberto B
authors.initials: GB
authors.lastname: Brito
authors.forename: Sérgio L A
authors.initials: SL
authors.lastname: Brienze
authors.forename: Carla B
authors.initials: CB
authors.lastname: Nonino
authors.forename: Antonio C
authors.initials: AC
authors.lastname: Brandão
authors.forename: Dorotéia R S
authors.initials: DR
authors.lastname: Souza
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001057:Apolipoproteins E / Q000235:genetics* / Q000378:metabolism; D015992:Body Mass Index; D001938:Brazil / Q000453:epidemiology; D016022:Case-Control Studies; D002769:Cholelithiasis / Q000453:epidemiology / Q000235:genetics* / Q000378:metabolism; D053480:Cholesterol Ester Transfer Proteins / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005500:Follow-Up Studies; D015390:Gastric Bypass; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D009767:Obesity, Morbid / Q000453:epidemiology / Q000235:genetics* / Q000378:metabolism / Q000601:surgery*; D012150:Polymorphism, Restriction Fragment Length; D012307:Risk Factors; D055815:Young Adult
chemical_list: D001057:Apolipoproteins E; D053480:Cholesterol Ester Transfer Proteins