title: Volume-stable adipose tissue formation by implantation of human adipose-derived stromal cells using solid free-form fabrication-based polymer scaffolds.
abstract: Regeneration of volume-stable adipose tissue is required for treatment of soft-tissue loss due to cancer, trauma, burns and for correctional cosmetic surgery. In this study, we hypothesized that transplantation of human adipose-derived stromal cells (hADSCs) using polycaprolactone (PCL) scaffolds fabricated with a solid free-form fabrication method would better maintain the volume of regenerated adipose tissues, as compared with the use of fibrin gel. Six weeks after implantation into the dorsal subcutaneous pockets of athymic mice, the volumes and adipose tissue areas of hADSC-PCL scaffold implants were significantly larger than those of hADSC-fibrin implants. In addition, the mRNA expression of adipogenic genes was more extensive in the hADSC-PCL scaffold implants.
authors.affiliation: Department of Bioengineering, Hanyang University, Seongdong-gu, Seoul, Republic of Korea.
authors.forename: Tae-Jin
authors.initials: TJ
authors.lastname: Lee
authors.forename: Suk Ho
authors.initials: SH
authors.lastname: Bhang
authors.forename: Wan-Guen
authors.initials: WG
authors.lastname: La
authors.forename: Sun-Hyun
authors.initials: SH
authors.lastname: Kwon
authors.forename: Jung-Youn
authors.initials: JY
authors.lastname: Shin
authors.forename: Hee Hun
authors.initials: HH
authors.lastname: Yoon
authors.forename: Heungsoo
authors.initials: H
authors.lastname: Shin
authors.forename: Dong-Woo
authors.initials: DW
authors.lastname: Cho
authors.forename: Byung-Soo
authors.initials: BS
authors.lastname: Kim
mesh_terms: D050156:Adipogenesis / Q000235:genetics; D000818:Animals; D002478:Cells, Cultured; D005260:Female; D005337:Fibrin; D005819:Genetic Markers; D048091:Guided Tissue Regeneration / Q000379:methods*; D006801:Humans; D050155:Lipogenesis / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008855:Microscopy, Electron, Scanning; D011091:Polyesters; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017154:Stromal Cells / Q000637:transplantation*; D050151:Subcutaneous Fat / Q000166:cytology / Q000502:physiology*; D054457:Tissue Scaffolds
chemical_list: D005819:Genetic Markers; D011091:Polyesters; C016240:polycaprolactone; D005337:Fibrin