title: Anti-inflammatory effect of Spiranthera odoratissima A. St.-Hil. leaves involves reduction of TNF-α.
abstract: Spiranthera odoratissima A. St.-Hil., 'manacá', is a medicinal species used in Brazil, especially in central region, for the treatment of several diseases such as pain and inflammation. In this study, the methanol/aqueous phase of the ethanol extract of the leaves of 'manacá' (MAP), at the doses of 50, 150 and 500 mg/kg was used to evaluate the anti-inflammatory and/or antinociceptive effects and the possible anti-inflammatory mechanism. The antinociceptive and anti-inflammatory activities of MAP were assessed using formalin test, carrageenan-induced paw oedema. The myeloperoxidase activity, capillary permeability, leukocyte migration and tumour necrosis factor alpha (TNF-α) levels were evaluated in pleural exudate. The MAP reduced the licking time only in the later phase of formalin test, and showed anti-inflammatory activity by reducing the paw oedema, migration cell, myeloperoxidase activity, capillary permeability and TNF-α levels. In conclusion, we confirmed the inflammatory activity of MAP and affirm that this effect involves the reduction of TNF-α level.
authors.affiliation: Laboratory of Pharmacology of Natural Products, Institute of Biological Sciences, Federal University of Goias, Goiânia, Brazil. xico@icb.ufg.br
authors.forename: Marcus Vinícius M
authors.initials: MV
authors.lastname: Nascimento
authors.forename: Pablinny Moreira
authors.initials: PM
authors.lastname: Galdino
authors.forename: Iziara Ferreira
authors.initials: IF
authors.lastname: Florentino
authors.forename: Adriane Ferreira
authors.initials: AF
authors.lastname: de Brito
authors.forename: Frederico Argollo
authors.initials: FA
authors.lastname: Vanderlinde
authors.forename: José Realino
authors.initials: JR
authors.lastname: de Paula
authors.forename: Roberto do Nascimento
authors.initials: Rdo N
authors.lastname: Silva
authors.forename: Elson Alves
authors.initials: EA
authors.lastname: Costa
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000494:pharmacology*; D001938:Brazil; D002351:Carrageenan / Q000009:adverse effects; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D007962:Leukocytes / Q000187:drug effects; D051379:Mice; D009195:Peroxidase / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D027821:Rutaceae / Q000737:chemistry*; D023382:Toxicity Tests, Acute; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D002351:Carrageenan; D009195:Peroxidase