title: Anti-angiogenic activity of Morinda citrifolia extracts and its chemical constituents.
abstract: Morinda citrifolia L. has been used for the treatment of a wide variety of diseases, including cancer. This study was undertaken to evaluate the anti-angiogenic effect of M. citrifolia fruits and leaves. Anti-angiogenic activity was evaluated in vivo using the chick chorioallantoic membrane assay. Bioactivity-guided fractionation and isolation were performed to identify the active constituent, and high-performance liquid chromatography analysis was then used to quantify the amount of this active constituent in the active extracts and fraction. The methanol extracts of fruits and leaves of M. citrifolia and the subsequent chloroform fraction of the fruit methanolic extract were found to have potential anti-angiogenic activity and were more potent compared to suramin. Scopoletin was identified as one of the chemical constituents that may be partly responsible for the anti-angiogenic activity of M. citrifolia fruits. The present findings further support the use of M. citrifolia in cancer or other pathological conditions related to angiogenesis.
authors.affiliation: School of Pharmaceutical Sciences, Universiti Sains Malaysia, Penang, Malaysia. khengbeh@yahoo.com
authors.forename: Hooi-Kheng
authors.initials: HK
authors.lastname: Beh
authors.forename: Lay-Jing
authors.initials: LJ
authors.lastname: Seow
authors.forename: Mohd Zaini
authors.initials: MZ
authors.lastname: Asmawi
authors.forename: Amin Malik Shah
authors.initials: AM
authors.lastname: Abdul Majid
authors.forename: Vikneswaran
authors.initials: V
authors.lastname: Murugaiyah
authors.forename: Norhayati
authors.initials: N
authors.lastname: Ismail
authors.forename: Zhari
authors.initials: Z
authors.lastname: Ismail
mesh_terms: D020533:Angiogenesis Inhibitors / Q000737:chemistry* / Q000494:pharmacology*; D000818:Animals; D049033:Chorioallantoic Membrane / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D005638:Fruit / Q000737:chemistry; D032066:Morinda / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D020533:Angiogenesis Inhibitors; D010936:Plant Extracts