title: Analysis of neofructooligosaccharides production mediated by the extracellular β-fructofuranosidase from Xanthophyllomyces dendrorhous.
abstract: The extracellular β-fructofuranosidase Xd-INV from the yeast Xanthophyllomyces dendrorhous mainly synthesizes the neo-fructooligosaccharides (neo-FOS) neokestose and neonystose. This enzyme is a glycoprotein with a content of 59-67% N-linked carbohydrates and an estimated molecular mass of 160-200 kDa. The extent level of glycosylation affects the thermal behaviour of the enzyme but not its hydrolase and transferase activities, which are optimal at 60-70 °C. The neo-FOS yield of this enzyme was increased from 40 to 168 g/L when the sucrose concentration increased from 420 to 600 g/L and when the reaction was carried out at 60 °C. The neo-FOS levels obtained (168 g/L) in this work are the largest reported for any microbial β-fructofuranosidase.
authors.affiliation: Centro de Biología Molecular Severo Ochoa, Departamento de Biología Molecular (CSIC-UAM), Universidad Autónoma Madrid, 28049 Madrid, Spain.
authors.forename: Dolores
authors.initials: D
authors.lastname: Linde
authors.forename: Barbara
authors.initials: B
authors.lastname: Rodríguez-Colinas
authors.forename: Marta
authors.initials: M
authors.lastname: Estévez
authors.forename: Ana
authors.initials: A
authors.lastname: Poveda
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Plou
authors.forename: María
authors.initials: M
authors.lastname: Fernández Lobato
mesh_terms: D001487:Basidiomycota / Q000201:enzymology*; D055162:Biocatalysis; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005110:Extracellular Space / Q000201:enzymology*; D006031:Glycosylation; D006867:Hydrolases / Q000378:metabolism; D007700:Kinetics; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000737:chemistry; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013395:Sucrose / Q000032:analysis / Q000737:chemistry; D013696:Temperature; D013997:Time Factors; D014166:Transferases / Q000378:metabolism; D014312:Trisaccharides / Q000096:biosynthesis / Q000737:chemistry; D043324:beta-Fructofuranosidase / Q000378:metabolism*
chemical_list: D009844:Oligosaccharides; D014312:Trisaccharides; C116580:fructooligosaccharide; C072434:neokestose; D013395:Sucrose; D014166:Transferases; D006867:Hydrolases; D043324:beta-Fructofuranosidase; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase