title: Identification and genomovar assignation of clinical strains of Pseudomonas stutzeri.
abstract: The identification of Pseudomonas stutzeri clinical isolates through conventional phenotypic methods was compared with identification through partial rpoD gene sequencing. We observed that commercial phenotypic systems easily confuse P. stutzeri with other Pseudomonas species. We also demonstrated that most of the clinical strains of P. stutzeri herein studied (79%) belonged to genomovar 1 of the species. We propose the use of partial rpoD gene sequence analysis as a complementary molecular tool for the precise routine identification and genomovar assignation of P. stutzeri clinical isolates, as well as for typing and epidemiological studies.
authors.affiliation: Microbiología, Departamento de Biología, Universidad de las Islas Baleares, 07122, Palma de Mallorca, Islas Baleares, España. clau.scotta@gmail.com
authors.forename: C
authors.initials: C
authors.lastname: Scotta
authors.forename: M
authors.initials: M
authors.lastname: Mulet
authors.forename: D
authors.initials: D
authors.lastname: Sánchez
authors.forename: M
authors.initials: M
authors.lastname: Gomila
authors.forename: A
authors.initials: A
authors.lastname: Ramírez
authors.forename: A
authors.initials: A
authors.lastname: Bennasar
authors.forename: E
authors.initials: E
authors.lastname: García-Valdés
authors.forename: B
authors.initials: B
authors.lastname: Holmes
authors.forename: J
authors.initials: J
authors.lastname: Lalucat
mesh_terms: D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D017720:Molecular Epidemiology / Q000379:methods; D008969:Molecular Sequence Data; D058889:Molecular Typing; D010802:Phylogeny; D011552:Pseudomonas Infections / Q000382:microbiology*; D044223:Pseudomonas stutzeri / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D012321:DNA-Directed RNA Polymerases