title: Highly selective cysteine detection and bioimaging in zebrafish through emission color change of water-soluble conjugated polymer-based assay complex.
abstract: A new concept for rapid, label-free cysteine sensing method is proposed via possible naked eye-detection of red-to-blue emission color change. Intermolecular exciton migration in conjugated polyelectrolyte-based assay complex is adopted to enhance selectivity and sensitivity for cysteine sensing by formation and dissociation of polymer-Hg(2+)-thymine assay complex in the absence and presence of cysteine, respectively. The assay complex shows red emission due to cooperative aggregation of conjugated polyelectrolyte, thymine, and Hg(2+). Upon exposure to cysteine, the assay complex dissociates into individual molecules showing transparent, blue-emitting solution, because cysteine extracts Hg(2+) from the assay complex via more favorable binding between cysteine and Hg(2+).
authors.affiliation: Organic and Optoelectronic Materials Laboratory, Department of Advanced Organic Materials and Textile System Engineering, Chungnam National University, Daejeon 305-764, Korea.
authors.forename: Na Young
authors.initials: NY
authors.lastname: Kwon
authors.forename: Daigeun
authors.initials: D
authors.lastname: Kim
authors.forename: Geunseok
authors.initials: G
authors.lastname: Jang
authors.forename: Jung Hyo
authors.initials: JH
authors.lastname: Lee
authors.forename: Ju-Hoon
authors.initials: JH
authors.lastname: So
authors.forename: Cheol-Hee
authors.initials: CH
authors.lastname: Kim
authors.forename: Tae Hyeon
authors.initials: TH
authors.lastname: Kim
authors.forename: Taek Seung
authors.initials: TS
authors.lastname: Lee
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000379:methods*; D003116:Color; D003545:Cysteine / Q000378:metabolism*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008858:Microscopy, Phase-Contrast; D058105:Polymerization; D011108:Polymers / Q000737:chemistry*; D012995:Solubility; D013050:Spectrometry, Fluorescence; D014867:Water / Q000737:chemistry*; D015027:Zebrafish / Q000378:metabolism*
chemical_list: D011108:Polymers; D014867:Water; D003545:Cysteine