title: Bone induction through controlled release of novel BMP-2-related peptide from PTMC₁₁-F127-PTMC₁₁ hydrogels.
abstract: Bone morphogenetic protein 2 (BMP-2) is the most powerful osteogenic factor; its effectiveness in enhancing osteoblastic activation has been confirmed both in vitro and in vivo. We developed a novel peptide (designated P24) derived from the 'knuckle' epitope of BMP-2 and found it also had osteogenic bioactivity to some extent. The main objective of this study was to develop a controlled release system based on poly(trimethylene carbonate)-F127-poly(trimethylene carbonate) (PTMC₁₁-F127-PTMC₁₁) hydrogels for the P24 peptide, to promote bone formation. By varying the copolymer concentrations, we demonstrated that P24/PTMC₁₁-F127-PTMC₁₁ hydrogels were an efficient system for the sustained release of P24 over 21-35 days. The P24-loaded hydrogels elevated alkaline phosphatase activity and promoted the expression of osteocalcin mRNA in bone marrow stromal cells (BMSCs) in vitro. Radiographic and histological examination showed that P24-loaded hydrogels could induce more effective ectopic bone formation in vivo than P24-free hydrogels. These results indicate that the PTMC₁₁-F127-PTMC₁₁ hydrogel is a suitable carrier for the controlled release of P24, and is a promising injectable biomaterial for the induction of bone regeneration.
authors.affiliation: Department of Orthopaedics, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, People's Republic of China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Tang
authors.forename: Jingjing
authors.initials: J
authors.lastname: Zhao
authors.forename: Shuyun
authors.initials: S
authors.lastname: Xu
authors.forename: Jingfeng
authors.initials: J
authors.lastname: Li
authors.forename: Yu
authors.initials: Y
authors.lastname: Teng
authors.forename: Daping
authors.initials: D
authors.lastname: Quan
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Guo
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001846:Bone Development / Q000187:drug effects; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism*; D001861:Bone Regeneration / Q000187:drug effects; D001842:Bone and Bones; D002454:Cell Differentiation; D004146:Dioxanes / Q000737:chemistry; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D008422:Materials Testing; D015675:Osteocalcin / Q000378:metabolism; D010012:Osteogenesis; D010455:Peptides / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D051381:Rats; D013696:Temperature; D014057:Tomography, X-Ray Computed
chemical_list: C525718:BMP2 protein, human; D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D004146:Dioxanes; D020100:Hydrogels; D010455:Peptides; D011108:Polymers; D015675:Osteocalcin; C059299:polytrimethylene carbonate; D000469:Alkaline Phosphatase