title: Neuroanatomical distribution of disease-associated prion protein in experimental bovine spongiform encephalopathy in cattle after intracerebral inoculation.
abstract: The pathologic disease-associated prion protein (PrP(Sc)) has been shown to be expressed in  the central nervous system of Holstein cattle inoculated intracerebrally with 3 sources of classical  bovine spongiform encephalopathy (BSE) isolates. Several regions of the brain and spinal cord were  analyzed for PrP(Sc) expression by immunohistochemical and Western blotting analyses. Animals  euthanized at 10 months post-inoculation (mpi) showed PrP(Sc) deposits in the brainstem and thalamus,  but no vacuolation; this suggested that the BSE agent might exhibit area-dependent tropism in the  brain. At 16 and 18 mpi, a small amount of vacuolation was detected in the brainstem and thalamus,  but not in the cerebral cortices. At 20 to 24 mpi, when clinical symptoms were apparent, heavy PrP(Sc)  deposits were evident throughout the brain and spinal cord. The mean time to the appearance of  clinical symptoms was 19.7 mpi, and the mean survival time was 22.7 mpi. These findings show that  PrP(Sc) accumulation was detected approximately 10 months before the clinical symptoms of BSE became  apparent. In addition, the 3 sources of BSE prion induced no detectable differences in the clinical  signs, incubation periods, neuroanatomical location of vacuoles, or distribution and pattern of  PrP(Sc) depositions in the brain.
authors.affiliation: Animal Research Center, Hokkaido Research Organization, Hokkaido, Japan.
authors.forename: Shigeo
authors.initials: S
authors.lastname: Fukuda
authors.forename: Sadao
authors.initials: S
authors.lastname: Onoe
authors.forename: Satoshi
authors.initials: S
authors.lastname: Nikaido
authors.forename: Kei
authors.initials: K
authors.lastname: Fujii
authors.forename: Soichi
authors.initials: S
authors.lastname: Kageyama
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Iwamaru
authors.forename: Morikazu
authors.initials: M
authors.lastname: Imamura
authors.forename: Kentaro
authors.initials: K
authors.lastname: Masujin
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Matsuura
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Shimizu
authors.forename: Kazuo
authors.initials: K
authors.lastname: Kasai
authors.forename: Miyako
authors.initials: M
authors.lastname: Yoshioka
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Murayama
authors.forename: Shirou
authors.initials: S
authors.lastname: Mohri
authors.forename: Takashi
authors.initials: T
authors.lastname: Yokoyama
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Okada
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001933:Brain Stem / Q000378:metabolism / Q000473:pathology*; D002417:Cattle; D016643:Encephalopathy, Bovine Spongiform / Q000378:metabolism / Q000473:pathology*; D005260:Female; D007150:Immunohistochemistry; D018620:PrPSc Proteins / Q000008:administration & dosage / Q000032:analysis / Q000378:metabolism*; D013116:Spinal Cord / Q000378:metabolism / Q000473:pathology*; D013788:Thalamus / Q000378:metabolism / Q000473:pathology; D013997:Time Factors; D014617:Vacuoles / Q000378:metabolism / Q000473:pathology
chemical_list: D018620:PrPSc Proteins