title: Selective discrimination of small hydrophobic biomolecules based on ion-current rectification in conically shaped nanochannel.
abstract: In this study, based on ion-current rectification in the conically shaped nanochannel embedded in polyethylene terephthalate (PET) membrane, we have selectively discriminated three small biomolecules. Because three positive biomolecules (Hoechst 33342, Propidium and Bupivacaine) have different hydrophobicities, their interactions with inside wall of the conical nanochannel are different and their binding affinities can be derived from Langmuir absorption model. Therefore, we can successfully discriminate these small biomolecules. The highest binding constant was obtained for the small molecule with highest hydrophobicity. Another interesting result is that the detection limit for the small molecule with the highest binding constant shifts to submicromole.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Guo
authors.forename: Jiahai
authors.initials: J
authors.lastname: Wang
authors.forename: Erkang
authors.initials: E
authors.lastname: Wang
mesh_terms: D001562:Benzimidazoles / Q000032:analysis; D002045:Bupivacaine / Q000032:analysis; D005591:Chemical Fractionation / Q000295:instrumentation / Q000379:methods*; D004553:Electric Conductivity; D004563:Electrochemistry; D057927:Hydrophobic and Hydrophilic Interactions; D017136:Ion Transport; D057230:Limit of Detection; D008567:Membranes, Artificial; D049329:Nanostructures; D011093:Polyethylene Terephthalates / Q000737:chemistry; D016062:Porosity; D011419:Propidium / Q000032:analysis
chemical_list: D001562:Benzimidazoles; D008567:Membranes, Artificial; D011093:Polyethylene Terephthalates; D011419:Propidium; C017807:bisbenzimide ethoxide trihydrochloride; D002045:Bupivacaine