title: A method for regenerating gold surface for prolonged reuse of gold-coated surface plasmon resonance chip.
abstract: We developed a method to completely regenerate the gold (Au) surface of 3-aminopropyltriethoxysilane (APTES)-functionalized Au-coated surface plasmon resonance (SPR) chip that had been used for human fetuin A (HFA) immunoassay. It involved treatment of the used SPR chip with freshly prepared piranha solution (concentrated H(2)SO(4)/30% H(2)O(2)=3:1, v/v) for 15 min followed by extensive rinsing with ethanol and deionized water. The developed method enabled prolonged reuse of the regenerated SPR chip that increased its cost-effectiveness without affecting the reproducibility of HFA immunoassays.
authors.affiliation: Centre for Bioanalytical Sciences, National Centre for Sensor Research, Dublin City University, Dublin 9, Ireland. sandeep.vashist@nus.edu.sg
authors.forename: Sandeep Kumar
authors.initials: SK
authors.lastname: Vashist
mesh_terms: D006046:Gold / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay; D011437:Propylamines; D040081:Protein Array Analysis; D012821:Silanes / Q000737:chemistry; D020349:Surface Plasmon Resonance; D013499:Surface Properties; D060749:alpha-2-HS-Glycoprotein / Q000032:analysis / Q000276:immunology
chemical_list: D011437:Propylamines; D012821:Silanes; D060749:alpha-2-HS-Glycoprotein; D006046:Gold; C477625:amino-propyl-triethoxysilane