title: High-resolution biosensor based on localized surface plasmons.
abstract: We report on a new biosensor with localized surface plasmons (LSP) based on an array of gold nanorods and the total internal reflection imaging in polarization contrast. The sensitivity of the new biosensor is characterized and a model detection of DNA hybridization is carried out. The results are compared with a reference experiment using a conventional high-resolution surface plasmon resonance (SPR) biosensor. We show that the LSP-based biosensor delivers the same performance as the SPR system while involving significantly lower surface densities of interacting molecules. We demonstrate a limit of detection of 100 pM and a surface density resolution of only 35 fg×mm-2 that corresponds to less than one DNA molecule per nanoparticle on average.
authors.affiliation: Institute of Photonics and Electronics, Academy of Sciences of the Czech Republic, Chaberská 57, Prague, Czech Republic.
authors.forename: Marek
authors.initials: M
authors.lastname: Piliarik
authors.forename: Hana
authors.initials: H
authors.lastname: Sípová
authors.forename: Pavel
authors.initials: P
authors.lastname: Kvasnička
authors.forename: Nicolle
authors.initials: N
authors.lastname: Galler
authors.forename: Joachim R
authors.initials: JR
authors.lastname: Krenn
authors.forename: Jiří
authors.initials: J
authors.lastname: Homola
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D004247:DNA / Q000032:analysis* / Q000235:genetics*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D017403:In Situ Hybridization / Q000295:instrumentation*; D020349:Surface Plasmon Resonance / Q000295:instrumentation*
chemical_list: D004247:DNA