title: Co-solvent mediated thermal stabilization of chondroitinase ABC I form Proteus vulgaris.
abstract: Chondroitinase ABC I (cABC I) from Proteus vulgaris cleaves glycosaminoglycan chains which are responsible for most of the inhibition of axon regrowth in spinal cord injury. The clinical utilization of this enzyme is mainly limited by its thermal instability. This study has been undertaken to determine the effects of glycerol, sorbitol and trehalose on cABC I activity and thermal stability. The results indicated that the enzyme catalytic activity and intrinsic fluorescence intensity increased in the presence of these cosolvents whereas no considerable conformational changes observed in far-UV CD spectra. Thermal CD experiment revealed an increase in T(m) of cABC I in the presence of cosolvents which was significant for trehalose. Our results support the idea that cABC I has stabilized in the presence of glycerol, sorbitol and trehalose. Therefore, the use of these cosolvents seems to be promising for improvement in shelf-life and clinical applications of this drug enzyme.
authors.affiliation: Department of Clinical Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mahdieh
authors.initials: M
authors.lastname: Nazari-Robati
authors.forename: Khosro
authors.initials: K
authors.lastname: Khajeh
authors.forename: Mahdi
authors.initials: M
authors.lastname: Aminian
authors.forename: Mehrnoosh
authors.initials: M
authors.lastname: Fathi-Roudsari
authors.forename: Abolfazl
authors.initials: A
authors.lastname: Golestani
mesh_terms: D019765:Chondroitin ABC Lyase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D003001:Cloning, Molecular; D004795:Enzyme Stability / Q000187:drug effects; D005990:Glycerol / Q000494:pharmacology; D011489:Protein Denaturation / Q000187:drug effects; D011514:Proteus vulgaris / Q000201:enzymology*; D012997:Solvents / Q000494:pharmacology*; D013012:Sorbitol / Q000494:pharmacology; D013696:Temperature; D013997:Time Factors; D014199:Trehalose / Q000494:pharmacology
chemical_list: D012997:Solvents; D013012:Sorbitol; D014199:Trehalose; D019765:Chondroitin ABC Lyase; D005990:Glycerol