title: The newly isolated endophytic fungus Paraconiothyrium sp. LK1 produces ascotoxin.
abstract: We have isolated five endophytic fungi from the roots of Capsicum annuum, Cucumis sativus and Glycine max. The culture filtrates (CF) of these endophytes were screened on dwarf mutant rice (Waito-C) and normal rice (Dongjin-byeo). Endophyte CAC-1A significantly inhibited the growth of Waito-C and Dongjin-byeo. Endophyte CAC-1A was identified as Paraconiothyrium sp. by sequencing the ITS rDNA region and phylogenetic analysis. The ethyl acetate fraction of Paraconiothyrium sp. suppressed the germination of Lactuca sativa and Echinochloa crus-galli seeds. The ethyl acetate fraction of the endophyte was subjected to bioassay-guided isolation and we obtained the phytotoxic compound ascotoxin (1) which was characterized through NMR and GC/MS techniques. Ascotoxin revealed 100% inhibitory effects on seed germination of Echinochloa crus-galli. Compound (1) was isolated for the first time from Paraconiothyrium sp.
authors.affiliation: School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University, Daegu 702-701, Korea.
authors.forename: Abdul Latif
authors.initials: AL
authors.lastname: Khan
authors.forename: Muhammad
authors.initials: M
authors.lastname: Hamayun
authors.forename: Javid
authors.initials: J
authors.lastname: Hussain
authors.forename: Sang-Mo
authors.initials: SM
authors.lastname: Kang
authors.forename: In-Jung
authors.initials: IJ
authors.lastname: Lee
mesh_terms: D020126:Brefeldin A / Q000378:metabolism* / Q000494:pharmacology; D031747:Echinochloa / Q000187:drug effects / Q000502:physiology; D060026:Endophytes / Q000302:isolation & purification* / Q000378:metabolism; D018525:Germination / Q000187:drug effects; D006540:Herbicides / Q000378:metabolism* / Q000494:pharmacology; D018545:Lactuca / Q000187:drug effects / Q000502:physiology; D012275:Oryza / Q000187:drug effects / Q000502:physiology; D010802:Phylogeny; D010936:Plant Extracts / Q000378:metabolism* / Q000494:pharmacology; D018517:Plant Roots / Q000382:microbiology; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D004718:Saccharomycetales / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D017422:Sequence Analysis, DNA
chemical_list: D006540:Herbicides; D010936:Plant Extracts; D012337:RNA, Ribosomal, 18S; D020126:Brefeldin A