title: CD40 triggering induces strong cytotoxic T lymphocyte responses to heat-killed Staphylococcus aureus immunization in mice: a new vaccine strategy for staphylococcal mastitis.
abstract: Staphylococcus (S.) aureus is a major pathogen involved in chronic bovine mastitis. Staphylococcal mastitis is difficult to control due to the ability of S. aureus to invade and survive within host cells. We therefore postulated that induction of CD8(+) cytotoxic T lymphocyte (CTL) responses leading to destruction of infected cells could help in the control of S. aureus mastitis. We demonstrate that immunization of mice with heat-killed S. aureus together with agonistic anti-CD40 monoclonal antibodies elicits strong CTL responses capable of reducing the severity of subsequent staphylococcal mastitis. Our study shows promise for CTL-dependent vaccination against S. aureus mastitis.
authors.affiliation: Laboratory of Cellular and Molecular Physiology, University of Liège, 1 Avenue de l'Hôpital (B34), Sart Tilman, Liège, B-4000, Belgium.
authors.forename: Hugues
authors.initials: H
authors.lastname: Wallemacq
authors.forename: Denis
authors.initials: D
authors.lastname: Bedoret
authors.forename: Julien
authors.initials: J
authors.lastname: Pujol
authors.forename: Christophe
authors.initials: C
authors.lastname: Desmet
authors.forename: Pierre-Vincent
authors.initials: PV
authors.lastname: Drion
authors.forename: Frédéric
authors.initials: F
authors.lastname: Farnir
authors.forename: Jacques
authors.initials: J
authors.lastname: Mainil
authors.forename: Pierre
authors.initials: P
authors.lastname: Lekeux
authors.forename: Fabrice
authors.initials: F
authors.lastname: Bureau
authors.forename: Laurence
authors.initials: L
authors.lastname: Fiévez
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000276:immunology; D019013:CD40 Antigens / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D004195:Disease Models, Animal; D008413:Mastitis / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013203:Staphylococcal Infections / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D013209:Staphylococcal Vaccines / Q000008:administration & dosage / Q000276:immunology*; D013211:Staphylococcus aureus / Q000276:immunology* / Q000472:pathogenicity; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D019013:CD40 Antigens; D013209:Staphylococcal Vaccines; D015164:Vaccines, Inactivated