title: Identification of hydrophobic tags for the degradation of stabilized proteins.
abstract: New HyTs are a knockout: we previously reported that labeling HaloTag proteins with low molecular weight hydrophobic tags (HyTs) leads to targeted degradation of HaloTag fusion proteins. In this report, we employed a chemical approach to extend this hydrophobic tagging methodology to highly stabilized proteins by synthesizing and evaluating a library of HyTs, which led to the identification of HyT36.
authors.affiliation: Department of Molecular Cellular and Developmental Biology, Yale University, 219 Prospect Street, New Haven, CT 06511, USA.
authors.forename: Hyun Seop
authors.initials: HS
authors.lastname: Tae
authors.forename: Thomas B
authors.initials: TB
authors.lastname: Sundberg
authors.forename: Taavi K
authors.initials: TK
authors.lastname: Neklesa
authors.forename: Devin J
authors.initials: DJ
authors.lastname: Noblin
authors.forename: Jeffrey L
authors.initials: JL
authors.lastname: Gustafson
authors.forename: Anke G
authors.initials: AG
authors.lastname: Roth
authors.forename: Kanak
authors.initials: K
authors.lastname: Raina
authors.forename: Craig M
authors.initials: CM
authors.lastname: Crews
mesh_terms: D057809:HEK293 Cells; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D015394:Molecular Structure; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D011993:Recombinant Fusion Proteins