title: Flow cytometry of fluorescent proteins.
abstract: Fluorescent proteins are now a critical tool in all areas of biomedical research. In this article, we review the techniques required to use fluorescent proteins for flow cytometry, concentrating specifically on the excitation and emission requirements for each protein, and the specific equipment required for optimal use.
authors.affiliation: Experimental Transplantation and Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. telfordw@mail.nih.gov
authors.forename: William G
authors.initials: WG
authors.lastname: Telford
authors.forename: Teresa
authors.initials: T
authors.lastname: Hawley
authors.forename: Fedor
authors.initials: F
authors.lastname: Subach
authors.forename: Vladislav
authors.initials: V
authors.lastname: Verkhusha
authors.forename: Robert G
authors.initials: RG
authors.lastname: Hawley
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D002478:Cells, Cultured; D004926:Escherichia coli / Q000235:genetics; D005434:Flow Cytometry / Q000379:methods*; D005456:Fluorescent Dyes; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000737:chemistry*; D006801:Humans; D008164:Luminescent Proteins / Q000737:chemistry*; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D000097573:Red Fluorescent Protein
chemical_list: D001426:Bacterial Proteins; D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D000097573:Red Fluorescent Protein; C413663:fluorescent protein 583; C054562:yellow fluorescent protein, Bacteria