title: Connections between sphingosine kinase and phospholipase D in the abscisic acid signaling pathway in Arabidopsis.
abstract: Phosphatidic acid (PA) and phytosphingosine 1-phosphate (phyto-S1P) both are lipid messengers involved in plant response to abscisic acid (ABA). Our previous data indicate that PA binds to sphingosine kinase (SPHK) and increases its phyto-S1P-producing activity. To understand the cellular and physiological functions of the PA-SPHK interaction, we isolated Arabidopsis thaliana SPHK mutants sphk1-1 and sphk2-1 and characterized them, together with phospholipase Dα1 knock-out, pldα1, in plant response to ABA. Compared with wild-type (WT) plants, the SPHK mutants and pldα1 all displayed decreased sensitivity to ABA-promoted stomatal closure. Phyto-S1P promoted stomatal closure in sphk1-1 and sphk2-1, but not in pldα1, whereas PA promoted stomatal closure in sphk1-1, sphk2-1, and pldα1. The ABA activation of PLDα1 in leaves and protoplasts was attenuated in the SPHK mutants, and the ABA activation of SPHK was reduced in pldα1. In response to ABA, the accumulation of long-chain base phosphates was decreased in pldα1, whereas PA production was decreased in SPHK mutants, compared with WT. Collectively, these results indicate that SPHK and PLDα1 act together in ABA response and that SPHK and phyto-S1P act upstream of PLDα1 and PA in mediating the ABA response. PA is involved in the activation of SPHK, and activation of PLDα1 requires SPHK activity. The data suggest that SPHK/phyto-S1P and PLDα1A are co-dependent in amplification of response to ABA, mediating stomatal closure in Arabidopsis.
authors.affiliation: Department of Biology, University of Missouri, St. Louis, Missouri 63121, USA.
authors.forename: Liang
authors.initials: L
authors.lastname: Guo
authors.forename: Girish
authors.initials: G
authors.lastname: Mishra
authors.forename: Jennifer E
authors.initials: JE
authors.lastname: Markham
authors.forename: Maoyin
authors.initials: M
authors.lastname: Li
authors.forename: Amanda
authors.initials: A
authors.lastname: Tawfall
authors.forename: Ruth
authors.initials: R
authors.lastname: Welti
authors.forename: Xuemin
authors.initials: X
authors.lastname: Wang
mesh_terms: D000040:Abscisic Acid / Q000235:genetics / Q000378:metabolism*; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D004789:Enzyme Activation / Q000502:physiology; D055785:Gene Knockdown Techniques; D010712:Phosphatidic Acids / Q000235:genetics / Q000378:metabolism; D010739:Phospholipase D / Q000235:genetics / Q000378:metabolism*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000378:metabolism*; D054046:Plant Stomata / Q000201:enzymology* / Q000235:genetics; D015398:Signal Transduction / Q000502:physiology*; D013110:Sphingosine / Q000031:analogs & derivatives / Q000235:genetics / Q000378:metabolism; D000099157:Sphingosine Kinase
chemical_list: D000040:Abscisic Acid; D029681:Arabidopsis Proteins; D010712:Phosphatidic Acids; D010739:Phospholipase D; D017853:Phosphotransferases (Alcohol Group Acceptor); D013110:Sphingosine; D000099157:Sphingosine Kinase; C117422:phytosphingosine-1-phosphate; C106801:phospholipase D1