title: Ultra-high resolution in MAS solid-state NMR of perdeuterated proteins: implications for structure and dynamics.
abstract: High resolution proton spectra are obtained in MAS solid-state NMR in case samples are prepared using perdeuterated protein and D(2)O in the recrystallization buffer. Deuteration reduces drastically (1)H, (1)H dipolar interactions and allows to obtain amide proton line widths on the order of 20 Hz. Similarly, high-resolution proton spectra of aliphatic groups can be obtained if specifically labeled precursors for biosynthesis of methyl containing side chains are used, or if limited amounts of H(2)O in the bacterial growth medium is employed. This review summarizes recent spectroscopic developments to access structure and dynamics of biomacromolecules in the solid-state, and shows a number of applications to amyloid fibrils and membrane proteins.
authors.affiliation: Munich Center for Integrated Protein Science (CIPSM), Department Chemie, Technische Universität München, Lichtenbergstr. 4, 85747 Garching, Germany. reif@tum.de
authors.forename: Bernd
authors.initials: B
authors.lastname: Reif
mesh_terms: D000465:Algorithms; D000544:Alzheimer Disease / Q000378:metabolism; D000577:Amides / Q000737:chemistry; D000595:Amino Acid Sequence; D000682:Amyloid / Q000737:chemistry; D016229:Amyloid beta-Peptides / Q000737:chemistry; D016880:Anisotropy; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry; D001436:Bacteriorhodopsins / Q000737:chemistry; D003903:Deuterium; D029968:Escherichia coli Proteins / Q000737:chemistry; D017665:Hydroxyl Radical; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008565:Membrane Proteins / Q000737:chemistry; D008958:Models, Molecular; D018272:Porins / Q000737:chemistry; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry*; D011522:Protons
chemical_list: D000577:Amides; D000682:Amyloid; D016229:Amyloid beta-Peptides; D001425:Bacterial Outer Membrane Proteins; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; C114347:OmpG protein, E coli; D018272:Porins; D011506:Proteins; D011522:Protons; D017665:Hydroxyl Radical; D001436:Bacteriorhodopsins; D003903:Deuterium