title: Phenotypic and functional maturation of murine dendritic cells (DCs) induced by purified Glycyrrhizin (GL).
abstract: The aim of this study is to investigate phenotypic and functional modulation of murine dendritic cells (DCs) with use of purified Glycyrrhizin (GL). These impacts of GL on DCs both from bone marrow derived DCs and established DC cell 2.4 were assessed with conventional scanning electron microscopy (SEM), flow cytometry (FCM), transmission electron microscopy (TEM), cytochemistry assay, FITC-dextran, bio-assay and enzyme linked immunosorbent assay (ELISA). We found that the purified GL induced phenotypic maturation as evidenced by increased expression of CD86, CD40, CD80, CD83 and major histocompatibility complex II (MHC II). The functional tests showed the activity of acidic phosphatase (ACP) inside the DCs2.4 cells were down- regulated after treatment with GL (which occurs when phagocytosis of DCs2.4 cells were decreased). Finally, we proved that GL increased the production of IL-12, IL-10 and decreased the production of tumor necrosis factor alpha (TNF-α). These data indicated that GL could promote maturation of DCs and this adjuvant-like activity may have potential therapeutic value. It is therefore concluded that GL could exert positive modulation on murine DCs.
authors.affiliation: Department of Immunology, School of Basic Medical Science, China Medical University, No.92, North Second Road, Heping District, Shenyang 110001, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Hua
authors.forename: Zaifu
authors.initials: Z
authors.lastname: Liang
authors.forename: Weiwei
authors.initials: W
authors.lastname: Li
authors.forename: Yiming
authors.initials: Y
authors.lastname: Meng
authors.forename: Xuan
authors.initials: X
authors.lastname: Li
authors.forename: Zhenjie
authors.initials: Z
authors.lastname: Zhang
authors.forename: Changlong
authors.initials: C
authors.lastname: Lu
authors.forename: Jingjuan
authors.initials: J
authors.lastname: Meng
authors.forename: Fengping
authors.initials: F
authors.lastname: Shan
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D022162:Cytoplasmic Vesicles / Q000187:drug effects / Q000648:ultrastructure; D003713:Dendritic Cells / Q000187:drug effects* / Q000648:ultrastructure; D004789:Enzyme Activation / Q000502:physiology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D019695:Glycyrrhizic Acid / Q000494:pharmacology*; D016753:Interleukin-10 / Q000096:biosynthesis; D018664:Interleukin-12 / Q000096:biosynthesis; D051379:Mice; D008810:Mice, Inbred C57BL; D008855:Microscopy, Electron, Scanning; D010587:Phagocytosis / Q000187:drug effects; D010641:Phenotype; D010873:Pinocytosis / Q000187:drug effects; D018124:Receptors, Tumor Necrosis Factor / Q000096:biosynthesis / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D018124:Receptors, Tumor Necrosis Factor; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D018664:Interleukin-12; D019695:Glycyrrhizic Acid; D000135:Acid Phosphatase