title: Electrochemical impedimetric biosensor based on a nanostructured polycarbonate substrate.
abstract: This study integrates the techniques of nanoelectroforming, hot-embossing, and electrochemical deposition to develop a disposable, low-cost, and high sensitivity nanostructure biosensor. A modified anodic aluminum oxide barrier-layer surface was used as the template for thin nickel film deposition. After etching the anodic aluminum oxide template off, a three-dimensional mold of the concave nanostructure array was created. The fabricated three-dimensional nickel mold was further used for replica molding of a nanostructure polycarbonate substrate by hot-embossing. A thin gold film was then sputtered onto the polycarbonate substrate to form the electrode, followed by deposition of an orderly and uniform gold nanoparticle layer on the three-dimensional gold electrode using electrochemical deposition. Finally, silver nanoparticles were deposited on the uniformly deposited gold nanoparticles to enhance the conductivity of the sensor. Electrochemical impedance spectroscopy analysis was then used to detect the concentration of the target element. The sensitivity of the proposed scheme on the detection of the dust mite antigen, Der p2, reached 0.1 pg/mL.
authors.affiliation: Department of Mechanical Engineering, National Chung-Hsing University, Taichung, Taiwan.
authors.forename: Yu-Shan
authors.initials: YS
authors.lastname: Chen
authors.forename: Chia-Che
authors.initials: CC
authors.lastname: Wu
authors.forename: Jaw-Ji
authors.initials: JJ
authors.lastname: Tsai
authors.forename: Gou-Jen
authors.initials: GJ
authors.lastname: Wang
mesh_terms: D000537:Aluminum Oxide / Q000737:chemistry; D039741:Antigens, Dermatophagoides / Q000032:analysis; D060829:Arthropod Proteins / Q000032:analysis; D015374:Biosensing Techniques / Q000295:instrumentation*; D058266:Dielectric Spectroscopy / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D006046:Gold / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure*; D036103:Nanotechnology; D011076:Polycarboxylate Cement / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012834:Silver / Q000737:chemistry
chemical_list: D039741:Antigens, Dermatophagoides; D060829:Arthropod Proteins; C451163:Dermatophagoides pteronyssinus antigen p 2; D011076:Polycarboxylate Cement; C005506:polycarbonate; D012834:Silver; D006046:Gold; D000537:Aluminum Oxide