title: Effects of a calcium phosphate cement on mineralized nodule formation compared with endodontic cements.
abstract: The aim of this study was to investigate mineralizing ability of a premixed calcium phosphate cement (premixed-CPC) compared to mineral trioxide aggregate (MTA) and zinc oxide eugenol cement (SuperEBA) in ROS17/2.8 cells. The measurements of cell proliferation, alkaline phosphatase (ALPase) activity and mineralized nodule formation in the presence or absence (control) of the test materials were performed using a cell culture insert method with the test materials placed on a porous membrane of culture plate insert. Mineralized nodules were detected by staining with alizarin red, and the calcium content of the mineralized nodules was determined quantitatively using a calcium assay kit. Premixed-CPC and MTA indicated significantly higher cell proliferation, ALPase activity, mineralized nodule formation, and calcium content in nodules than those of SuperEBA (p<0.05). The present results suggest that premixed-CPC has the same mineralizing ability as MTA.
authors.affiliation: Department of Endodontics, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-8310, Japan.
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Ogata
authors.forename: Makoto
authors.initials: M
authors.lastname: Hayashi
authors.forename: Hiromasa
authors.initials: H
authors.lastname: Tsuda
authors.forename: Naoto
authors.initials: N
authors.lastname: Suzuki
authors.forename: Masao
authors.initials: M
authors.lastname: Maeno
authors.forename: Akiyoshi
authors.initials: A
authors.lastname: Sugawara
authors.forename: Bunnai
authors.initials: B
authors.lastname: Ogiso
mesh_terms: D000469:Alkaline Phosphatase / Q000187:drug effects; D017607:Aluminum Compounds / Q000494:pharmacology*; D000818:Animals; D000880:Anthraquinones; D002113:Calcification, Physiologic / Q000187:drug effects*; D002118:Calcium / Q000032:analysis; D017610:Calcium Compounds / Q000494:pharmacology*; D002130:Calcium Phosphates / Q000494:pharmacology*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004396:Coloring Agents; D003738:Dental Cements / Q000494:pharmacology*; D017220:Dentin-Bonding Agents / Q000494:pharmacology; D004338:Drug Combinations; D008422:Materials Testing; D010006:Osteoblasts / Q000187:drug effects*; D010012:Osteogenesis / Q000187:drug effects; D010087:Oxides / Q000494:pharmacology*; D011506:Proteins / Q000032:analysis; D051381:Rats; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D017640:Silicates / Q000494:pharmacology*; D015035:Zinc Oxide-Eugenol Cement / Q000494:pharmacology*
chemical_list: D017607:Aluminum Compounds; D000880:Anthraquinones; D017610:Calcium Compounds; D002130:Calcium Phosphates; D004396:Coloring Agents; D003738:Dental Cements; D017220:Dentin-Bonding Agents; D004338:Drug Combinations; D010087:Oxides; D011506:Proteins; D012387:Root Canal Filling Materials; D017640:Silicates; D015035:Zinc Oxide-Eugenol Cement; C086631:mineral trioxide aggregate; C004468:Alizarin Red S; C084074:Super EBA; C020243:calcium phosphate; D000469:Alkaline Phosphatase; D002118:Calcium