title: [Effect of cellulose-decomposing strain on microbial community of cow manure compost].
abstract: Taking the cow dung and straw as composting raw materials, effect of cellulose-decomposing strain on microbial community of cow manure compost was investigated with the traditional culture method and PCR-DGGE technique. The results showed that the microbiological inocula showed a more rapid rate of temperature elevation at the start of composting and prolonged the time of high-temperature process and increased the number of microbial. The DGGE map of cellulose-decomposing strain compost was different from natural compost, the succession of microbial community in cellulose-decomposing strain was faster than natural compost. Sequence comparison revealed that the Pseudomonas sp. of bacterial appeared at the initial stage and Acinetobacter sp., Flavobacteria were existed at the high-temperature process in natural compost; while Arthrobacter sp. was appeared at the high-temperature process in cellulose-decomposing strain compost. Bacillus sp. was dominant species at middle and later stage in natural compost and cellulose-decomposing strain compost. Eimeriidae of fungal appeared in compost materials, Aspergillus and thermophilic fungi were dominant species at the high-temperature process in natural compost and cellulose-decomposing strain compost. Ascomycota appeared at middle and later stage in natural compost; while Basidiomycetes in cellulose-decomposing strain compost. Aspergillus was found throughout the process. This result suggested that the microbiological inocula were able to facilitate the bacterial microbial diversity of the compost; reduced the fungal microbial diversity of the compost. The aims of this study were to provide a scientific basis to the diversity of microbial community by monitoring the dynamics of microbial community in cellulose-decomposing strain compost and represent an important step towards the understanding of microbiological inocula and its function in the degradation process of compost.
authors.affiliation: College of Resources and Environmental Sciences, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.forename: Wan
authors.initials: W
authors.lastname: Li
authors.forename: Xiu-Hong
authors.initials: XH
authors.lastname: Xu
authors.forename: Hong-Tao
authors.initials: HT
authors.lastname: Li
mesh_terms: D000383:Agriculture / Q000379:methods*; D000818:Animals; D001173:Arthrobacter / Q000254:growth & development / Q000378:metabolism; D001407:Bacillus / Q000254:growth & development / Q000378:metabolism; D001419:Bacteria / Q000145:classification* / Q000254:growth & development / Q000378:metabolism; D002417:Cattle; D002482:Cellulose / Q000378:metabolism*; D015169:Colony Count, Microbial; D005658:Fungi / Q000254:growth & development / Q000378:metabolism; D008372:Manure; D012275:Oryza / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry*
chemical_list: D008372:Manure; D002482:Cellulose