title: Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for species identification of bacteria of genera Arcanobacterium and Trueperella.
abstract: In the present study matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was evaluated for species identification of 98 bacteria previously classified phenotypically and genotypically to genera Arcanobacterium and Trueperella. Species identification was carried out by comparing the main spectra of each strain with the main spectra of reference strains of both genera and 3740 database entries included in the MALDI Biotyper 2.0 software package (Bruker Daltonik GmbH, Bremen, Germany). MALDI-TOF MS correctly identified (log (score) values ≥ 2.0) all investigated strains of the species A. (T.) bialowiezense (n=3), A. (T.) bonasi (n=7), A. haemolyticum (n=10), A. pluranimalium (n=1) and A. (T.) pyogenes (n=77). According to the present results MALDI-TOF MS had a comparable discriminating power than previously conducted tests on DNA level. Further studies with strains isolated from human infections would show the robustness of MALDI-TOF MS for identification of bacteria of these genera.
authors.affiliation: Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Giessen, Frankfurter Str. 107, 35392 Giessen, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Hijazin
authors.forename: A A
authors.initials: AA
authors.lastname: Hassan
authors.forename: J
authors.initials: J
authors.lastname: Alber
authors.forename: C
authors.initials: C
authors.lastname: Lämmler
authors.forename: M
authors.initials: M
authors.lastname: Timke
authors.forename: M
authors.initials: M
authors.lastname: Kostrzewa
authors.forename: E
authors.initials: E
authors.lastname: Prenger-Berninghoff
authors.forename: M
authors.initials: M
authors.lastname: Zschöck
mesh_terms: D000191:Actinomycetaceae / Q000145:classification* / Q000235:genetics; D000818:Animals; D055667:Arcanobacterium / Q000145:classification* / Q000235:genetics; D012680:Sensitivity and Specificity; D012984:Software; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*