title: [Establishment of a multiplex PCR detection method for four foodborne pathogens].
abstract: OBJECTIVE
  To establish a multiplex PCR method for simultaneous detection of Salmonella, Shigella, Staphylococcus aureus and Listeria monocytogenes in food.
  
  
  METHODS
  The primers were designed according to the sequences of invA gene of Salmonella, ipaH gene of Shigella, nuc gene of Staphylococcus aureus and hly gene of Listeria monocytogenes, and the reaction system was optimized.
  
  
  RESULTS
  No false positive and false negative results occurred during the test of 15 target strains and 17 non-target strains of bacteria, and the molecular weight of PCR products were consistent with expected. The detection limits for each pathogen were at least 1 pg/microl. The sequence analysis of PCR products showed the same sequences with target genes. The test result of 319 samples showed that 4 raw milk samples were contaminated by staphylococcus aureus, and 1 raw pork sample by Salmonella.
  
  
  CONCLUSIONS
  The established method showed good specificity, and provided an important method for the rapid detection of foodborne pathogens.
authors.affiliation: Zhejiang Test Academy of Quality and Technical Supervision, Hangzhou 310013, China.
authors.forename: Kan
authors.initials: K
authors.lastname: Jiang
authors.forename: Donglei
authors.initials: D
authors.lastname: Zhang
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Jin
authors.forename: Xiaozhen
authors.initials: X
authors.lastname: Chen
mesh_terms: D005506:Food Contamination / Q000032:analysis; D005516:Food Microbiology; D008089:Listeria monocytogenes / Q000235:genetics / Q000302:isolation & purification; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D012475:Salmonella / Q000235:genetics / Q000302:isolation & purification*; D012760:Shigella / Q000235:genetics / Q000302:isolation & purification*; D013211:Staphylococcus aureus / Q000235:genetics / Q000302:isolation & purification*