title: Burkholderia and Cupriavidus spp. are the preferred symbionts of Mimosa spp. in southern China.
abstract: Rhizobia were isolated from invasive Mimosa spp. (M. diplotricha and M. pudica) in Dehong district of the province of Yunnan in subtropical southern China. Almost all of the 98 isolates were β-rhizobia in the genera Burkholderia and Cupriavidus. These strains were analysed for their distribution characteristics together with strains from a previous study from Sishuangbanna. The proportion of nodules containing each β-rhizobial genus varied between Mimosa species, with Cupriavidus being predominant in M. diplotricha nodules (63.3% compared to 36.7% occupation with Burkholderia), but with M. pudica showing a slight preference for Burkholderia over Cupriavidus, with them occupying 56.5% and 43.5% of nodules, respectively. The symbiosis-essential genes nodA and nifH were present in all the Burkholderia and Cupriavidus strains tested, and their phylogenies indicated that these Mimosa symbionts share symbiotic genes with native South American rhizobia. The evolutionary discrepancies among 16S rRNA genes, nodA and nifH of Mimosa spp. symbionts, suggests that the nod and nif genes of β-rhizobia evolved independently.
authors.affiliation: Key Laboratory of Microbial Diversity Research and Application of Hebei Province, College of Life Sciences, Hebei University, Baoding, China. liuxiaoyunly@126.com
authors.forename: XiaoYun
authors.initials: X
authors.lastname: Liu
authors.forename: Shuang
authors.initials: S
authors.lastname: Wei
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Euan K
authors.initials: EK
authors.lastname: James
authors.forename: XiaoYe
authors.initials: X
authors.lastname: Guo
authors.forename: Catherine
authors.initials: C
authors.lastname: Zagar
authors.forename: Liu Gui
authors.initials: LG
authors.lastname: Xia
authors.forename: Xin
authors.initials: X
authors.lastname: Dong
authors.forename: Yi Peng
authors.initials: YP
authors.lastname: Wang
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D019117:Burkholderia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D002681:China; D049921:Cupriavidus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D014644:Genetic Variation; D058865:Introduced Species; D031298:Mimosa / Q000382:microbiology* / Q000502:physiology; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics; D010802:Phylogeny; D012988:Soil Microbiology; D013559:Symbiosis / Q000502:physiology; D013624:Taiwan
chemical_list: D001426:Bacterial Proteins; D010088:Oxidoreductases; C029130:nitrogenase reductase