title: The protective effect of 17β-estradiol against hydrogen peroxide-induced apoptosis on mesenchymal stem cell.
abstract: This study was designed to investigate the function of 17β-estradiol (17β-E2) against oxidative stress on the cell death of mice bone marrow mesenchymal stem cells (BMSCs) induced by hydrogen peroxide (H₂O₂). BMSCs were treated with 17β-E2 for 24h and then treated with 100μM H₂O₂ for 1h. Cell viability, apoptosis, caspase-9 mRNA, JNKs (Jun N-terminal kinases) and c-Jun protein expression in BMSCs were evaluated. Cell apoptosis of BMSCs were increased in a dose-dependent manner after treated with H₂O₂ compared to control group. But pretreatment with 17β-E2 can inhibit apoptosis of BMSCs, preserve the mitochondrial transmembrane potential, decrease caspase-9 mRNA, JNK1/2 and c-Jun protein expression. In conclusion, 17β-E₂ exerts antiapoptotic effects in BMSCs which related to the mitochondria death pathway and JNKs pathway. The study revealed that 17β-E₂ can reduce the donor stem cells damage.
authors.affiliation: Central Laboratory for Experimental Medicine, Liaocheng People's Hospital and Liaocheng Clinical School of Taishan Medical University, Shandong Province, Liaocheng 252000, China.
authors.forename: Hai-Ying
authors.initials: HY
authors.lastname: Chen
authors.forename: Xian
authors.initials: X
authors.lastname: Zhang
authors.forename: Shuang-Feng
authors.initials: SF
authors.lastname: Chen
authors.forename: Ying-Xin
authors.initials: YX
authors.lastname: Zhang
authors.forename: Yi-Hua
authors.initials: YH
authors.lastname: Liu
authors.forename: Long-Le
authors.initials: LL
authors.lastname: Ma
authors.forename: Le-Xin
authors.initials: LX
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001854:Bone Marrow Cells / Q000187:drug effects / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000008:administration & dosage / Q000494:pharmacology*; D004967:Estrogens / Q000008:administration & dosage / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000633:toxicity; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects*
chemical_list: D004967:Estrogens; D004958:Estradiol; D006861:Hydrogen Peroxide; D048031:JNK Mitogen-Activated Protein Kinases; D053453:Caspase 9