title: Simultaneous laser-induced fluorescence, coaxial thermal lens spectroscopy and retro-reflected beam interference detection for capillary electrophoresis.
abstract: A simultaneous laser-induced fluorescence (LIF), coaxial thermal lens spectroscopy (TLS) and retro-reflected beam interference (RBI) detection for capillary electrophoresis (CE), has been described. In its optical scheme, a diode-pump solid-state (DPSS) laser was employed as the pump laser in both LIF detection and coaxial TLS detection, and a He-Ne laser was utilized as the probe laser in coaxial TLS detection and RBI detection. In addition, RBI signals with and without thermal lens had been theoretically compared to ensure the reliability of the RBI signal. Moreover, the focus length of the key lens has been optimized to improve the performance of the proposed detection. The last but not least, several determinations were taken to evaluate its limit of detection, linear range as well as relative standard deviation, all of which indicate no worse results compared with former reports. LIF and coaxial TLS detection owned high sensitivity, and RBI detection indicated versatile property, based on which the reported detection achieved a sensitive and universal detection for CE.
authors.affiliation: College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Xiong
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Xueyan
authors.initials: X
authors.lastname: Miao
authors.forename: Lijia
authors.initials: L
authors.lastname: Liu
authors.forename: Liping
authors.initials: L
authors.lastname: Wang
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zhou
authors.forename: Jiming
authors.initials: J
authors.lastname: Hu