title: Musa paradisica RCI complements AtRCI and confers Na+ tolerance and K+ sensitivity in Arabidopsis.
abstract: The mechanisms involved in Na⁺/K⁺ uptake and extrusion are important in plant salt tolerance. In this study, we investigated the physiological role of a plasma membrane (PM)-localized protein, MpRCI, from plantain in transgenic Arabidopsis under NaCl and KCl stress and determined its effect on PM fluidity and H⁺-ATPase activity. The MpRCI gene exhibited high homology to the AtRCI2 gene family in Arabidopsis and was therefore able to complement for loss of the yeast AtRCI2-related PMP3 gene. Results of phenotypic espial and atomic emission spectrophotometer (AES) assays indicated that MpRCI overexpression in the AtRCI2A knockout mutant with reduced shoot Na⁺ and increased K⁺ exhibited increased Na⁺-tolerance and K⁺-sensitivity under NaCl or KCl treatments, respectively. Furthermore, comparisons of PM fluidity and H⁺-ATPase activity in shoots, with expression or absence of MpRCI/AtRCI2A expression under NaCl or KCl stress, showed MpRCI maintained PM fluidity and H⁺-ATPase activity under stress conditions. Results suggest that MpRCI plays an essential role in Na⁺/K⁺ flux in plant cells.
authors.affiliation: Guangdong Key Laboratory of Plant Resources, State Key Laboratory for Biocontrol and Key Laboratory of Gene Engineering of Ministry of Education, School of Life Sciences, Sun Yat-sen University, 510275, Guangzhou, PR China.
authors.forename: Bing
authors.initials: B
authors.lastname: Liu
authors.forename: Dongru
authors.initials: D
authors.lastname: Feng
authors.forename: Bipei
authors.initials: B
authors.lastname: Zhang
authors.forename: Peiqiang
authors.initials: P
authors.lastname: Mu
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yanming
authors.initials: Y
authors.lastname: He
authors.forename: Kangbiao
authors.initials: K
authors.lastname: Qi
authors.forename: Jinfa
authors.initials: J
authors.lastname: Wang
authors.forename: Hongbin
authors.initials: H
authors.lastname: Wang
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics*; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D017353:Gene Deletion; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics; D005816:Genetic Complementation Test; D006720:Homozygote; D008560:Membrane Fluidity / Q000187:drug effects; D005810:Multigene Family; D028521:Musa / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D010940:Plant Proteins / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011188:Potassium / Q000378:metabolism*; D011189:Potassium Chloride / Q000494:pharmacology; D011510:Proteolipids / Q000235:genetics; D006180:Proton-Translocating ATPases / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D055051:Salt-Tolerant Plants / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D012964:Sodium / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology
chemical_list: C410735:PMP3 protein, S cerevisiae; D010940:Plant Proteins; D011510:Proteolipids; D029701:Saccharomyces cerevisiae Proteins; D012965:Sodium Chloride; D011189:Potassium Chloride; D012964:Sodium; D006180:Proton-Translocating ATPases; D011188:Potassium