title: Overexpression of four and a half LIM domains protein 2 promotes epithelial-mesenchymal transition-like phenotype in fish pre-osteoblasts.
abstract: FHL2 is a multifunctional protein involved in gene transcription regulation and cytoarchitecture modulation that has been recently associated with epithelial-mesenchymal transition (EMT) in colon cancer. Overexpression of FHL2 in a fish pre-osteoblastic cell line promoted cell dedifferentiation and impaired its extracellular matrix mineralization capacity. Cell cultures also acquired a novel three-dimensional structure organization, their proliferation rate was enhanced and gene expression profile was altered in agreement with an EMT-like phenotype upon overexpression of FHL2. Altogether, our results provide additional support to the relevance of FHL2 for cell differentiation and its association with hallmarks of cancer phenotype.
authors.affiliation: Centre of Marine Sciences (CCMAR/CIMAR-LA), University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal.
authors.forename: Marta S
authors.initials: MS
authors.lastname: Rafael
authors.forename: Vincent
authors.initials: V
authors.lastname: Laizé
authors.forename: Cláudia
authors.initials: C
authors.lastname: Florindo
authors.forename: Serena
authors.initials: S
authors.lastname: Ferraresso
authors.forename: Luca
authors.initials: L
authors.lastname: Bargelloni
authors.forename: M Leonor
authors.initials: ML
authors.lastname: Cancela
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D049109:Cell Proliferation; D003094:Collagen / Q000378:metabolism; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics / Q000502:physiology*; D006025:Glycosaminoglycans / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D021541:Sea Bream / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D006025:Glycosaminoglycans; D014157:Transcription Factors; D003094:Collagen; D000469:Alkaline Phosphatase