title: Profilin as an aeroallergen by means of conjunctival allergen challenge with purified date palm profilin.
abstract: BACKGROUND
  The role of profilin as a food allergen is well established, but little research has been done about its ability to elicit respiratory disease. Profilin is considered more of a confounding allergen on skin testing with whole pollen extracts than other airborne allergens. Our aim was to find out whether or not profilin can cause symptoms in sensitized individuals, which would be compatible with its role as an airborne allergen.
  
  
  METHODS
  We performed conjunctival allergen challenges with date palm profilin in a series of consecutive pollen-allergic patients with rhino-conjunctivitis, divided in two groups: profilin sensitized (n = 17) and not sensitized (n = 14), who served as controls. We investigated the possible association between profilin sensitization and profilin allergy in these groups of patients.
  
  
  RESULTS
  None of the patients from the not profilin-sensitized group had a positive result in conjunctival allergen challenges. In contrast, 65% of profilin sensitized patients had a positive conjunctival allergen challenge and were considered allergic to profilin. We found a significant statistical association between being profilin allergic and being profilin sensitized (χ(2) = 10.39, p < 0.005).
  
  
  CONCLUSIONS
  Profilin seems to work as an aeroallergen in a significant proportion of profilin-sensitized patients. This might explain the uselessness of conjunctival challenges with whole pollen extracts to disclose genuine sensitization. In the future, the possibility of quantifying this allergen in pollen immunotherapy vaccines should be considered.
authors.affiliation: Allergy Department, Xeral-Calde Hospital, Lugo, Spain. ramon.nuñez.orjales@sergas.es
authors.forename: R
authors.initials: R
authors.lastname: Núñez
authors.forename: F
authors.initials: F
authors.lastname: Carballada
authors.forename: M
authors.initials: M
authors.lastname: Lombardero
authors.forename: L
authors.initials: L
authors.lastname: Jimeno
authors.forename: M
authors.initials: M
authors.lastname: Boquete
mesh_terms: D000293:Adolescent; D000328:Adult; D000485:Allergens; D028023:Arecaceae / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D003228:Conjunctiva / Q000276:immunology*; D005260:Female; D005512:Food Hypersensitivity / Q000276:immunology; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D051304:Profilins / Q000276:immunology*; D012882:Skin Tests; D055815:Young Adult
chemical_list: D000485:Allergens; D051304:Profilins