title: Superoxide dismutase activity of Helicobacter pylori per se from 158 clinical isolates and the characteristics.
abstract: We investigated the correlation between the SOD activity of Helicobacter pylori (H. pylori) and gastroduodenal diseases and the characteristics of strains exposed to oxidative stress. Two sequenced strains, 26695 and J99, and clinical isolates from 156 Japanese patients with gastroduodenal diseases such as gastric cancer (n= 59) and non-cancer (n= 97) were used. SOD activities of all 158 isolates were measured and were divided into three groups: high-SOD activity (>0.22, n= 2), moderate-SOD activity (0.15≦≦0.22, n= 16) and low-SOD activity (<0.15, n= 140). Expressions of H. pylori Fe-SOD were examined by western blotting with anti-H. pylori Fe-SOD antibody prepared inhouse, and the profiles of Fe-SOD activity were investigated by zymogram with activity staining in native-PAGE. The characteristics of strains from high-SOD and low-SOD groups were examined under oxidative stress by paraquat. The average of H. pylori SOD activity was significantly higher in the cancer group than in the non-cancer group (P < 0.05). However, irrespective of SOD activity level, the amount of Fe-SOD expressed was variable among individual strains. Zymogram revealed a single band in moderate-SOD and low-SOD strains, but multiple bands in high-SOD strains were observed. These bands were confirmed as H. pylori Fe-SOD. Under oxidative stress with paraquat, low-SOD strains were drastically eliminated without inducible SOD activity; however, high-SOD strains were still viable with increased SOD activity. This study is the first to exhibit the characteristics of high-SOD activity strains representing multiple bands in zymograms and the correlation between H. pylori SOD activity and gastric cancer.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Kochi Medical School, Kohasu, Oko-cho, Nankoku, Kochi 783-8505, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Morishita
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Takeuchi
authors.forename: Norihito
authors.initials: N
authors.lastname: Morimoto
authors.forename: Tomoko
authors.initials: T
authors.lastname: Shimamura
authors.forename: Yoshu
authors.initials: Y
authors.lastname: Kadota
authors.forename: Masayuki
authors.initials: M
authors.lastname: Tsuda
authors.forename: Taketoshi
authors.initials: T
authors.lastname: Taniguchi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ukeda
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Sugiura
mesh_terms: D000368:Aged; D044466:Asian People; D015153:Blotting, Western; D004381:Duodenal Ulcer / Q000382:microbiology / Q000473:pathology; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D020869:Gene Expression Profiling; D016481:Helicobacter Infections / Q000382:microbiology* / Q000473:pathology*; D016480:Helicobacter pylori / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D010269:Paraquat / Q000633:toxicity; D013274:Stomach Neoplasms / Q000382:microbiology / Q000473:pathology; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D013482:Superoxide Dismutase; D010269:Paraquat