title: Effect of angiotensin II on inflammation pathways in human primary bone cell cultures in otosclerosis.
abstract: BACKGROUND
  The aim of this study was to assess the expression and production of inflammation mediators in basal condition and after angiotensin II (AngII) in otosclerosis.
  
  
  METHODS
  Human stapedial cell cultures (6 otosclerosis and 6 controls) were incubated with AngII (10(-7)M, 24 h) or vehicle. Cytokines and their mRNA expression were assessed by antibody and cDNA arrays.
  
  
  RESULTS
  In basal conditions, otosclerotic cultures produced higher amounts of interleukin (IL)-1β and interferon-inducible protein 10, and smaller amounts of tissue inhibitor of metalloproteinase 2. AngII promoted inflammation by increasing interferon γ and IL-10, and by decreasing macrophage inflammatory protein 1α and soluble tumor necrosis factor receptor II.
  
  
  CONCLUSIONS
  Otosclerotic cultures produced higher proinflammatory cytokines in basal condition. AngII appeared to promote inflammation via these mediators in otosclerosis.
authors.affiliation: Inserm, UMRS, Paris-Diderot University, France.
authors.forename: Milan
authors.initials: M
authors.lastname: Rudić
authors.forename: Christine
authors.initials: C
authors.lastname: Nguyen
authors.forename: Yann
authors.initials: Y
authors.lastname: Nguyen
authors.forename: Lidija
authors.initials: L
authors.lastname: Milković
authors.forename: Neven
authors.initials: N
authors.lastname: Zarković
authors.forename: Olivier
authors.initials: O
authors.lastname: Sterkers
authors.forename: Evelyne
authors.initials: E
authors.lastname: Ferrary
authors.forename: Alexis
authors.initials: A
authors.lastname: Bozorg Grayeli
mesh_terms: D000328:Adult; D000804:Angiotensin II / Q000494:pharmacology*; D002478:Cells, Cultured; D054405:Chemokine CCL3 / Q000378:metabolism; D054357:Chemokine CXCL10 / Q000096:biosynthesis; D005260:Female; D006801:Humans; D007249:Inflammation / Q000378:metabolism* / Q000473:pathology; D007375:Interleukin-1 / Q000096:biosynthesis; D008297:Male; D008875:Middle Aged; D010011:Osteocytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D010040:Otosclerosis / Q000378:metabolism* / Q000473:pathology; D018124:Receptors, Tumor Necrosis Factor / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D013199:Stapes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000096:biosynthesis
chemical_list: D054405:Chemokine CCL3; D054357:Chemokine CXCL10; D007375:Interleukin-1; D018124:Receptors, Tumor Necrosis Factor; D000804:Angiotensin II; D019716:Tissue Inhibitor of Metalloproteinase-2