title: Coomassie blue is sufficient for specific protein detection of aptamer-conjugated chips.
abstract: An aptamer-based biochip for protein detection and quantitation which combines the recent biochip technology and the conventional staining methods, is described. Using a model system comprising His-tagged proteins as the analyte and single-stranded RNA aptamers specific for His-tagged proteins as immobilized ligands on chips, we could demonstrate that aptamers were equivalent or superior to antibodies in terms of specificity and sensitivity, respectively. The sensor has the characteristics of good stability, reproducibility and reusability, with detection limit as low as 85 ng/mL His-tagged protein. It has been demonstrated that the sensor can be stored for at least 4 weeks and reused with reasonable reduction rate of staining intensity. In conclusion, we could show the suitability of nucleic acid aptamers as low molecular weight receptors on biochips for sensitive and specific protein detection and quantitation.
authors.affiliation: Department of Chemistry, Research Institute for Basic Sciences, Kyung Hee University, Seoul, Republic of Korea.
authors.forename: Gwan-Ho
authors.initials: GH
authors.lastname: Lee
authors.forename: Sang Soo
authors.initials: SS
authors.lastname: Hah
mesh_terms: D052157:Aptamers, Nucleotide / Q000032:analysis* / Q000737:chemistry; D040081:Protein Array Analysis / Q000379:methods*; D015203:Reproducibility of Results; D012394:Rosaniline Dyes / Q000737:chemistry*
chemical_list: D052157:Aptamers, Nucleotide; D012394:Rosaniline Dyes; C048139:Coomassie blue