title: The contribution of side chains to antitumor activity of a polysaccharide from Codonopsis pilosula.
abstract: In order to assess the potential contribution of the side chains of a polysaccharide (CPPW1) from the roots of Codonopsis pilosula to the biological activities, we chose CPPW1 and its backbone (CPPW1B) as the research targets to compare them with their antitumor and immunomodulatory activity. The results demonstrated CPPW1 could significantly inhibit the tumor growth of H22-bearing mice and simulate lymphocyte proliferation. Meanwhile the phagocytic capability of macrophages and NO production were also enhanced. However, CPPW1B could only inhibit the tumor weight and did not work to immune system at all. Additionally, both CPPW1 and CPPW1B had no toxicity in vivo. Taken together with all data, we found the sugar side chains attached to the backbone of CPPW1 played a pivotal role in fighting against tumor and activating immune system.
authors.affiliation: School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China.
authors.forename: Chong
authors.initials: C
authors.lastname: Xu
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Guangxin
authors.initials: G
authors.lastname: Yuan
authors.forename: Ming
authors.initials: M
authors.lastname: Guan
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D029745:Codonopsis / Q000737:chemistry*; D005260:Female; D007155:Immunologic Factors / Q000737:chemistry* / Q000494:pharmacology*; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis; D010587:Phagocytosis / Q000187:drug effects; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology; D013329:Structure-Activity Relationship
chemical_list: D000970:Antineoplastic Agents; D007155:Immunologic Factors; D011134:Polysaccharides; D009569:Nitric Oxide