title: 5-Hydroxytryptamine receptor assays.
abstract: 5-Hydroxytryptamine (5-HT) receptors, by virtue of their broad expression pattern in peripheral and central tissues, regulate diverse physiological and behavioral responses through the activation of fourteen molecularly distinct receptor subtypes. The tissue-specific distribution of these receptors confers specificity for the actions of serotonin and highlights the therapeutic potential of serotonin receptor modulators. To better assess this therapeutic potential, it is useful to characterize serotonergic agonists and antagonists in physiologically relevant organ systems. Provided in this unit are twelve tissue bath assays using vascular and smooth muscle tissues isolated from guinea-pig, rat, and rabbit. These tests make possible the analyses of compounds at nine serotonin receptor subtypes.
authors.affiliation: Johnson & Johnson PRD, La Jolla, California, USA.
authors.forename: Katherine W
authors.initials: KW
authors.lastname: Figueroa
authors.forename: Graeme R
authors.initials: GR
authors.lastname: Martin
authors.forename: M Teresa
authors.initials: MT
authors.lastname: Pulido-Rios
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic; D001681:Biological Assay / Q000379:methods*; D002536:Cerebral Arteries; D004305:Dose-Response Relationship, Drug; D004558:Electric Stimulation / Q000379:methods; D005748:Gastric Fundus; D006168:Guinea Pigs; D007082:Ileum; D007601:Jugular Veins; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D011985:Receptors, Serotonin / Q000145:classification / Q000187:drug effects / Q000378:metabolism*; D017366:Serotonin Receptor Agonists / Q000494:pharmacology*; D013048:Specimen Handling
chemical_list: D011985:Receptors, Serotonin; D017366:Serotonin Receptor Agonists