title: SSR-based genetic maps of Miscanthus sinensis and M. sacchariflorus, and their comparison to sorghum.
abstract: We present SSR-based genetic maps from a cross between Miscanthus sacchariflorus Robustus and M. sinensis, the progenitors of the promising cellulosic biofuel feedstock Miscanthus × giganteus. cDNA-derived SSR markers were mapped by the two-way pseudo-testcross model due to the high heterozygosity of each parental species. A total of 261 loci were mapped in M. sacchariflorus, spanning 40 linkage groups and 1,998.8 cM, covering an estimated 72.7% of the genome. For M. sinensis, a total of 303 loci were mapped, forming 23 linkage groups and 2,238.3 cM, covering 84.9% of the genome. The use of cDNA-derived SSR loci permitted alignment of the Miscanthus linkage groups to the sorghum chromosomes, revealing a whole genome duplication affecting the Miscanthus lineage after the divergence of subtribes Sorghinae and Saccharinae, as well as traces of the pan-cereal whole genome duplication. While the present maps provide for many early research needs in this emerging crop, additional markers are also needed to improve map density and to further characterize the structural changes of the Miscanthus genome since its divergence from sorghum and Saccharum.
authors.affiliation: Plant Genome Mapping Laboratory, University of Georgia, 111 Riverbend Road, Rm 228, Athens, GA 30602, USA.
authors.forename: Changsoo
authors.initials: C
authors.lastname: Kim
authors.forename: Dong
authors.initials: D
authors.lastname: Zhang
authors.forename: Susan A
authors.initials: SA
authors.lastname: Auckland
authors.forename: Lisa K
authors.initials: LK
authors.lastname: Rainville
authors.forename: Katrin
authors.initials: K
authors.lastname: Jakob
authors.forename: Brent
authors.initials: B
authors.lastname: Kronmiller
authors.forename: Erik J
authors.initials: EJ
authors.lastname: Sacks
authors.forename: Martin
authors.initials: M
authors.lastname: Deuter
authors.forename: Andrew H
authors.initials: AH
authors.lastname: Paterson
mesh_terms: D002874:Chromosome Mapping / Q000379:methods*; D018744:DNA, Plant / Q000235:genetics; D008040:Genetic Linkage; D005819:Genetic Markers; D018895:Microsatellite Repeats; D031786:Saccharum / Q000235:genetics*; D016415:Sequence Alignment; D045868:Sorghum / Q000235:genetics*
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers