title: Inactivation of natural microorganisms in litchi juice by high-pressure carbon dioxide combined with mild heat and nisin.
abstract: The individual and combined effects of high-pressure carbon dioxide (HPCD), mild heat (MH) and nisin (200 ppm) on the inactivation of natural microorganisms, including aerobic bacteria (AB), yeasts and molds (Y&M), in litchi juice were evaluated. The samples were treated at a pressure of 10 MPa and temperatures of 32, 42 or 52 °C for 5, 10, 15, 20, 25 or 30 min. Temperature played a prominent role in the inactivation of both AB and Y&M when combined with HPCD, particularly for AB at 52 °C and Y&M at temperatures ≥42 °C. Nisin increased the susceptibility of AB to the combined treatment of HPCD and MH (HPCD + MH). A reduction of 4.19 log cycles was achieved by HPCD + MH at 52 °C for 15 min, and complete inactivation of AB was obtained by combination of HPCD, MH and nisin (HPCD + MH + nisin). No significant effect of nisin was found on the inactivation of Y&M.
authors.affiliation: College of Food Science and Nutritional Engineering, China Agricultural University, National Engineering Research Center for Fruit and Vegetable Processing, Key Lab of Fruit and Vegetable Processing, Ministry of Agriculture, Beijing 100083, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Liang
authors.initials: L
authors.lastname: Zhao
authors.forename: Jihong
authors.initials: J
authors.lastname: Wu
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Liao
mesh_terms: D001420:Bacteria, Aerobic / Q000187:drug effects / Q000254:growth & development; D001628:Beverages / Q000382:microbiology*; D002245:Carbon Dioxide / Q000378:metabolism*; D015169:Colony Count, Microbial; D005511:Food Handling / Q000379:methods; D005516:Food Microbiology / Q000379:methods; D005519:Food Preservation / Q000379:methods*; D005658:Fungi / Q000187:drug effects / Q000254:growth & development; D006358:Hot Temperature; D032125:Litchi / Q000382:microbiology*; D009561:Nisin / Q000494:pharmacology*; D011312:Pressure; D015003:Yeasts / Q000187:drug effects / Q000254:growth & development
chemical_list: D009561:Nisin; D002245:Carbon Dioxide