title: Administration of LPS-stimulated autologous macrophages induces α-synuclein aggregation in dopaminergic neurons of rat brain.
abstract: Administration of autologous macrophages isolated from the abdominal cavity and stimulated in vitro with bacterial LPS to rats increased blood level of antibodies against α-synuclein. Antibody titer reached its maximum during week 5 of the experiment and exceeded the levels of anti-α-synuclein antibodies appeared in response to transplantation of non-stimulated macrophages. Brain immunohistochemistry showed that additional administration of LPS (250 μg/kg) to animals during week 4 after injection of LPS-stimulated macrophages led to α-synuclein accumulation in 9.4±3.2% dopaminergic neurons of the substantia nigra. These findings attest to induction of humoral immune response to α-synuclein in rats after administration of autologous LPS-stimulated macrophages, which can affect α-synuclein metabolism in dopaminergic neurons of the brain.
authors.affiliation: Udmurt State University, Izhevsk, Russia.
authors.forename: T N
authors.initials: TN
authors.lastname: Sergeyeva
authors.forename: V G
authors.initials: VG
authors.lastname: Sergeyev
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood*; D001921:Brain / Q000378:metabolism; D059290:Dopaminergic Neurons / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000378:metabolism* / Q000494:pharmacology; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013378:Substantia Nigra / Q000378:metabolism; D051844:alpha-Synuclein / Q000276:immunology* / Q000378:metabolism
chemical_list: D000906:Antibodies; D008070:Lipopolysaccharides; D051844:alpha-Synuclein