title: Characterization of cannabinoid receptors.
abstract: This unit describes the use of cannabinoid radioligands in competitive binding assays for determining affinity parameters (IC(50), K(i)) of unlabeled compounds at cloned CB1 and CB2 receptors expressed in cells.
authors.affiliation: GlaxoSmithKline, Research Triangle Park, North Carolina, USA.
authors.forename: Richard F
authors.initials: RF
authors.lastname: Cox
authors.forename: Vincent
authors.initials: V
authors.lastname: Rash
mesh_terms: D001667:Binding, Competitive; D002186:Cannabinoids / Q000819:agonists* / Q000037:antagonists & inhibitors*; D006801:Humans; D020128:Inhibitory Concentration 50; D011869:Radioligand Assay / Q000379:methods*; D043884:Receptor, Cannabinoid, CB1 / Q000819:agonists / Q000032:analysis* / Q000037:antagonists & inhibitors; D043885:Receptor, Cannabinoid, CB2 / Q000819:agonists / Q000032:analysis* / Q000037:antagonists & inhibitors; D013048:Specimen Handling / Q000379:methods; D013997:Time Factors
chemical_list: D002186:Cannabinoids; D043884:Receptor, Cannabinoid, CB1; D043885:Receptor, Cannabinoid, CB2