title: [Determination of linuron and its metabolite 3,4-dichloroaniline residues in meat and meat products using liquid chromatography-tandem mass spectrometry].
abstract: A method of liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed for the determination of linuron and its metabolite 3, 4-dichloroaniline residues in pork, liver, kidney, casings, canned steam pork and sausage. The sample was extracted with a mixture of acetone-acetonitrile (5: 95, v/v). Most of the lipids in the extract were eliminated by freezing-lipid filtration. After Florisil solid phase extraction cleanup, the linuron and its metabolite residues were determined by LC-MS/MS, and quantified by internal standard meth-od. In the range of 1 - 500 microg/L, both linuron and 3,4-dichloroaniline showed good linearity with the correlation coefficients (r) more than 0. 998. The limit of quantification (S/N > 10) was 10 microg/kg, and the limit of detection (S/N > 3) was 5 microg/kg for each analyte. The recoveries of linuron and 3,4-dichloroaniline in meat and meat products at three spiked levels were in the ranges of 88.3% - 101.2% and 91.6% - 101. 6%, and the relative standard deviations were in the ranges of 4. 8% - 13. 7% and 4. 7% - 11. 8%, respectively. The results demonstrated that the proposed method can meet the requirements for the determination of linuron and 3,4-dichloroaniline in meat and meat products.
authors.affiliation: Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai 200135, China.
authors.forename: Dehua
authors.initials: D
authors.lastname: Guo
authors.forename: Xionghai
authors.initials: X
authors.lastname: Yi
authors.forename: Li
authors.initials: L
authors.lastname: Qu
mesh_terms: D000814:Aniline Compounds / Q000032:analysis*; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D006540:Herbicides / Q000032:analysis; D008044:Linuron / Q000032:analysis* / Q000378:metabolism; D008460:Meat / Q000032:analysis; D008461:Meat Products / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000814:Aniline Compounds; D006540:Herbicides; D010573:Pesticide Residues; D008044:Linuron; C014464:3,4-dichloroaniline