title: Bovine serum albumin significantly improves the DPPH free radical scavenging potential of dietary polyphenols and gallic acids.
abstract: Polyphenol-protein interaction (PPI) is reversible in that polyphenol-protein complex can be dissociated and release the free polyphenols. The aim of this study is to evaluate the contribution of polyphenol-protein interaction on improving the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging capacity of polyphenols. The DPPH radical scavenging potential of polyphenols was determined from 1 to 7 days under aerobic condition. The DPPH radical scavenging capacity of polyphenols depended on the structures of dietary polyphenols and gallic acids. The DPPH radical scavenging percentages of Group H polyphenols were weakened when kept in room temperature from 1 to 7 days. BSA rapid weakened the DPPH radical scavenging activity of polyphenols on the first day. However, the DPPH scavenging capacities of polyphenols in the presence of BSA overwhelmingly improved with increasing time. These results illustrated that BSA not only prolongs the effective time, but also improved the DPPH radical scavenging potential of polyphenols. The increasing DPPH scavenging percentages of polyphenols slightly decreased with increased affinities of BSA-polyphenol complexes.
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Cao
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Chen
authors.forename: Koichiro
authors.initials: K
authors.lastname: Yamamoto
mesh_terms: D000818:Animals; D001713:Biphenyl Compounds / Q000737:chemistry*; D002417:Cattle; D016166:Free Radical Scavengers / Q000737:chemistry*; D005707:Gallic Acid / Q000737:chemistry*; D015394:Molecular Structure; D010851:Picrates / Q000737:chemistry*; D059808:Polyphenols / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013997:Time Factors
chemical_list: D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010851:Picrates; D059808:Polyphenols; D012710:Serum Albumin, Bovine; D005707:Gallic Acid; C004931:1,1-diphenyl-2-picrylhydrazyl