title: Cuticular and internal n-alkane composition of Lucilia sericata larvae, pupae, male and female imagines: application of HPLC-LLSD and GC/MS-SIM.
abstract: The composition of cuticular and internal n-alkanes in Lucilia sericata larvae, pupae, and male and female imagines were studied. The cuticular and internal lipid extracts were separated by HPLC-LLSD, after which the hydrocarbon fraction was identified by GC/MS in selected ion monitoring (SIM) and total ion current (TIC) modes. The cuticular lipids of the larvae contained seven n-alkanes from C23 to C31. The major n-alkane in L. sericata larvae was C29 (42.1%). The total cuticular n-alkane content in the cuticular lipids was 31.46 μg g-1 of the insect body. The internal lipids of L. sericata larvae contained five n-alkanes ranged from C25 to C31. The most abundant compound was C27 (61.71 μg g-1 of the insect body). Eighteen n-alkanes from C14 to C31 were identified in the cuticular lipids of the pupae. The most abundant n-alkanes ranged from C25 to C31; those with odd-numbered carbon chains were particularly abundant, the major one being C29:0 (59.5%). Traces of eight cuticular n-alkanes were present. The internal lipids of L. sericata pupae contained five n-alkanes, ranging from C25 to C31. The cuticular lipids of female imagines contained 17 n-alkanes from C12 to C30. Among the cuticular n-alkanes of females, C27 (47.5%) was the most abundant compound. Four n-alkanes, with only odd-numbered carbon chains, were identified in the internal lipids of females. The lipids from both sexes of L. sericata had similar n-alkane profiles. The cuticular lipids of adult males contained 16 n-alkanes ranging from C13 to C31. C27 (47.9%) was the most abundant cuticular n-alkanes in males. The same n-alkanes only with odd-numbered carbon chains and in smaller quantities of C27 (0.1%) were also identified in the internal lipids of males. The highest amounts of total cuticular n-alkanes were detected in males and females of L. sericata (330.4 and 158.93 μg g-1 of the insect body, respectively). The quantities of total cuticular alcohols in larvae and pupae were smaller (31.46 μg g-1 and 42.08 μg g-1, respectively). The internal n-alkane contents of larvae, pupae, and male and female imagines were significantly higher than the cuticular n-alkane contents (153.53, 99.60, 360.06 and 838.76 μg g-1 of the insect body, respectively).
authors.affiliation: Institute for Environmental and Human Health Protection, Faculty of Chemistry, University of Gdańsk, ul. Sobieskiego 18/19, 80-952 Gdańsk, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Gołębiowski
authors.affiliation: Institute for Environmental and Human Health Protection, Faculty of Chemistry, University of Gdańsk, ul. Sobieskiego 18/19, 80-952 Gdańsk, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Paszkiewicz
authors.affiliation: Institute for Environmental and Human Health Protection, Faculty of Chemistry, University of Gdańsk, ul. Sobieskiego 18/19, 80-952 Gdańsk, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Grubba
authors.affiliation: Institute for Environmental and Human Health Protection, Faculty of Chemistry, University of Gdańsk, ul. Sobieskiego 18/19, 80-952 Gdańsk, Poland.
authors.forename: D
authors.initials: D
authors.lastname: Gąsiewska
authors.affiliation: Institute of Parasitology, Polish Academy of Sciences, Twarda 51/55, 00-818 Warsaw, Poland.
authors.forename: M I
authors.initials: MI
authors.lastname: Boguś
authors.affiliation: Institute of Parasitology, Polish Academy of Sciences, Twarda 51/55, 00-818 Warsaw, Poland.
authors.forename: E
authors.initials: E
authors.lastname: Włóka
authors.affiliation: Institute of Parasitology, Polish Academy of Sciences, Twarda 51/55, 00-818 Warsaw, Poland.
authors.forename: W
authors.initials: W
authors.lastname: Wieloch
authors.affiliation: Institute for Environmental and Human Health Protection, Faculty of Chemistry, University of Gdańsk, ul. Sobieskiego 18/19, 80-952 Gdańsk, Poland.
authors.forename: P
authors.initials: P
authors.lastname: Stepnowski
mesh_terms: D000473:Alkanes / Q000032:analysis*; D000818:Animals; D001823:Body Composition; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004175:Diptera / Q000737:chemistry*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D007814:Larva / Q000737:chemistry*; D008055:Lipids / Q000032:analysis; D008297:Male; D011679:Pupa / Q000737:chemistry*
chemical_list: D000473:Alkanes; D008055:Lipids