title: Electrochemical detection of hepatitis C viral NS3-4A protease.
abstract: Here we lay the ground work for the detection of hepatitis C viral NS3-4A protease exploiting peptide-protein interaction. The NS3-4A protease is inhibited by N-terminal cleavage products. Our approach is based on the formation of a self-assembled monolayer (SAM) of a ferrocene amino acid derivative on an electrode surface. A short NS3-4A specific inhibitory peptide (Asp-Glu-Ile-Val-Pro-Nva) was then covalently attached to the electrode surface. The interaction of the peptide, through the C-terminal, with the protein was quantified using electrochemical techniques. The systems exhibit a linear relationship between the measured signal and NS3-4A concentration in the range of 10-100 pM with a detection limit of 5 pM.
authors.affiliation: Department of Chemistry, The University of Western Ontario, 1151, Richmond Street, London, Ontario, Canada N6A 5B7.
authors.forename: Modupeola A
authors.initials: MA
authors.lastname: Sowole
authors.forename: Heinz-Bernhard
authors.initials: HB
authors.lastname: Kraatz
mesh_terms: D000595:Amino Acid Sequence; D004563:Electrochemistry / Q000379:methods*; D016174:Hepacivirus / Q000201:enzymology*; D008958:Models, Molecular; D009842:Oligopeptides / Q000378:metabolism / Q000494:pharmacology; D011487:Protein Conformation; D020349:Surface Plasmon Resonance / Q000379:methods*; D017361:Viral Nonstructural Proteins / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000378:metabolism; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
chemical_list: D009842:Oligopeptides; D017361:Viral Nonstructural Proteins; C084422:NS3 protein, hepatitis C virus; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases