title: Candida borneonana sp. nov., a methanol-assimilating anamorphic yeast isolated from decaying fruit.
abstract: Five strains of a previously uncharacterized anamorphic, methanol-assimilating yeast species are described here, for which the name Candida borneonana is proposed. The strains were isolated from fruit waste collected in markets in Brunei, Borneo. The sequences of the D1/D2 domains of the large subunit rRNA genes, the internal transcribed spacer (ITS) regions and the 18S rRNA genes were identical between the isolates and differed from the corresponding sequences of all previously described yeast species. Phylogenetic analysis of these sequences showed that the new species formed a cluster with species of the genus Kuraishia, the closest related species being K. capsulata (6 % nucleotide substitutions in the D1/D2 domain). The type strain, 11-487(T), has been deposited in the Centralbureau voor Schimmelcultures (Utrecht, The Netherlands) as CBS 12507(T), the Culture Collection of Yeasts (Bratislava, Slovakia) as CCY 29-182-1(T) and the National Collection of Agricultural and Industrial Microorganisms (Budapest, Hungary) as NCAIM Y.02008. Mycobank no. MB563710.
authors.affiliation: Department of Genetics and Applied Microbiology, University of Debrecen, Egyetem tér 1, 4032 Debrecen, Hungary.
authors.forename: Matthias
authors.initials: M
authors.lastname: Sipiczki
mesh_terms: D001893:Borneo; D002010:Brunei; D002175:Candida / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005638:Fruit / Q000382:microbiology*; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S / Q000235:genetics
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D012337:RNA, Ribosomal, 18S