title: Biocatalytic potential of an alkalophilic and thermophilic dextranase as a remedial measure for dextran removal during sugar manufacture.
abstract: The present study is focused on dextranase from Streptomyces sp. NK458 with potential to remove dextran formed during sugar manufacture. The dextranase had molecular weight of 130 kDa and hydrolyzed 15-25 and 410 kDa dextran. Dextranase production was optimized using statistical designs and the enzyme was purified 1.8-fold with 55.5% recovery. It displayed maximum activity at pH 9.0 and 60°C and was stable over a wide range of pH from 5.0 to 10.0. The k(m) and V(max) values were 3.05 mM and 17.97 mmol/ml/h, respectively. Ten units of dextranase could reduce dextran content by 67% in 24h and 56% in 72 h from sugarcane juice of cane variety CoS 86032. The enzyme was stable up to 3 days at 30°C beyond which its activity decreased and dextran removal could be retained by supplementation of 5 U of dextranase. These properties make it a promising biocatalyst for sugar industry.
authors.affiliation: Department of Microbiology, University of Pune, Pune 411007, India.
authors.forename: Shweta
authors.initials: S
authors.lastname: Purushe
authors.forename: Divya
authors.initials: D
authors.lastname: Prakash
authors.forename: Neelu N
authors.initials: NN
authors.lastname: Nawani
authors.forename: Prashant
authors.initials: P
authors.lastname: Dhakephalkar
authors.forename: Balasaheb
authors.initials: B
authors.lastname: Kapadnis
mesh_terms: D000468:Alkalies / Q000494:pharmacology*; D055162:Biocatalysis / Q000187:drug effects; D001673:Biodegradation, Environmental / Q000187:drug effects; D002241:Carbohydrates / Q000096:biosynthesis*; D003910:Dextranase / Q000302:isolation & purification / Q000378:metabolism*; D003911:Dextrans / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability / Q000187:drug effects; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D008954:Models, Biological; D013302:Streptomyces / Q000187:drug effects / Q000201:enzymology; D013696:Temperature
chemical_list: D000468:Alkalies; D002241:Carbohydrates; D003911:Dextrans; D003910:Dextranase