title: Neurogenic induction of human mesenchymal stem cells in fibrin 3D matrix.
abstract: We studied the potential of neurogenic induction of human bone marrow mesenchymal stem cells in fibrin-based 3D matrix. The best results were obtained after incubation of mesenchymal stem cells in fibrin 3D matrix in the presence of neurogenic medium containing EGF and bFGF growth factors. Under these conditions, most cells formed numerous branching processes and expressed neural cell marker βIII-tubulin. Optimal combination of 3D matrix and neurogenic factors of the medium can provide new insight into neurogenic potential of mesenchymal stem cells, which can be used for the therapy of neural traumas and neurodegenerative diseases.
authors.affiliation: Institute of Genetics and Cytology, National Academy of Sciences of Belarus, Minsk, Belarus. shakhbazau@gmail.com
authors.forename: A V
authors.initials: AV
authors.lastname: Shakhbazau
authors.forename: N V
authors.initials: NV
authors.lastname: Petyovka
authors.forename: S M
authors.initials: SM
authors.lastname: Kosmacheva
authors.forename: M P
authors.initials: MP
authors.lastname: Potapnev
mesh_terms: D001854:Bone Marrow Cells / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D003470:Culture Media; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005337:Fibrin; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D009474:Neurons / Q000166:cytology*; D054457:Tissue Scaffolds; D014404:Tubulin / Q000378:metabolism
chemical_list: D003470:Culture Media; D014404:Tubulin; D005346:Fibroblast Growth Factors; D004815:Epidermal Growth Factor; D005337:Fibrin