title: Identification of genes potentially involved in bone metastasis by genome-wide             gene expression profile analysis of non-small cell lung cancer in mice.
abstract: Lung cancer is commonly associated with multi-organ metastasis, and the             bone is a frequent metastatic site for lung cancer. However, the molecular mechanism             of organ-specific metastasis remains poorly understood. To elucidate this issue,             we analyzed in this study genome-wide gene expression profiles of 15 metastatic             lesions from three organs (bone, lung and liver) in a mouse model with multi-organ             metastasis properties of human non-small cell lung cancer cells (ACC-LC319/bone2),             using a combination of laser-microbeam microdissection and DNA microarrays. We             identified 299 genes that could potentially be involved in the organ-selective             nature of lung cancer metastasis. Among them, 77 were bone-specifically expressed             elements, including genes involved in cell adhesion, cytoskeleton/cell motility,             extracellular matrix remodeling and cell-cell signaling as well as genes already             known to be involved in the bone metastasis of breast cancers. Quantitative RT-PCR             confirmed the specific upregulation of eight genes in bone metastasis tumors,             suggesting that these genes may be involved in bone metastasis. Our findings should             be helpful for a better understanding of the molecular aspects of the metastatic             process in different organs, and could lead to molecular target-based anticancer             drugs and prevention of metastasis, especially bone metastasis.
authors.affiliation: Division of Genome Medicine, Institute for Genome Research, The                     University of Tokushima, Tokushima 770-8503, Japan.
authors.forename: Le Tan
authors.initials: le T
authors.lastname: Dat
authors.forename: Taisuke
authors.initials: T
authors.lastname: Matsuo
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Yoshimaru
authors.forename: Soji
authors.initials: S
authors.lastname: Kakiuchi
authors.forename: Hisatsugu
authors.initials: H
authors.lastname: Goto
authors.forename: Masaki
authors.initials: M
authors.lastname: Hanibuchi
authors.forename: Takuya
authors.initials: T
authors.lastname: Kuramoto
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Nishioka
authors.forename: Saburo
authors.initials: S
authors.lastname: Sone
authors.forename: Toyomasa
authors.initials: T
authors.lastname: Katagiri
mesh_terms: D000818:Animals; D001859:Bone Neoplasms / Q000235:genetics* / Q000556:secondary*; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000556:secondary*; D045744:Cell Line, Tumor; D016000:Cluster Analysis; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic; D020022:Genetic Predisposition to Disease; D055106:Genome-Wide Association Study; D006801:Humans; D061346:Laser Capture Microdissection; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology*; D008297:Male; D051379:Mice; D016513:Mice, SCID; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction