title: Rapid detection of neoplastic cells in serous cavity effusions in children with flow cytometry immunophenotyping.
abstract: The aim of the study was to evaluate the role of flow cytometric immunophenotyping (FCI) as a rapid diagnostic tool for pediatric malignancy in serous cavity effusions (SCEs). FCI results for 103 SCEs in a pediatric population were compared with retrospective clinical outcomes (RCOs). Among 41 patients assessed as having malignancies by RCO, 36 patients were diagnosed with lymphoma (n =25), acute myeloid leukemia (n =2), neuroblastoma (n =8) and retinoblastoma (n =1) by FCI, respectively. The sensitivity and specificity of FCI for detecting neoplastic cells were 87.8% and 98.4%, respectively. The concordance of FCI data with final diagnoses of the patients was 94.2%. FCI data for lymphoma was concordant with the final diagnosis in 89.3% of cases. When Hodgkin lymphoma was excluded, the overall correlation increased to 96.1%. FCI is a useful tool for rapid and reliable diagnosis of pediatric non-Hodgkin lymphoma in SCE samples and also to suggest the presence of non-lymphoid malignancies, especially neuroblastoma.
authors.affiliation: Division of Hematology-Oncology, Zhejiang Key Laboratory for Neonatal Diseases, the Children's Hospital of Zhejiang University School of Medicine, Hangzhou, China.
authors.forename: Hongqiang
authors.initials: H
authors.lastname: Shen
authors.forename: Yongmin
authors.initials: Y
authors.lastname: Tang
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Xu
authors.forename: Lie
authors.initials: L
authors.lastname: Wang
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Wang
authors.forename: Weiqun
authors.initials: W
authors.lastname: Xu
authors.forename: Hua
authors.initials: H
authors.lastname: Song
authors.forename: Zhengyan
authors.initials: Z
authors.lastname: Zhao
authors.forename: Jianli
authors.initials: J
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D006689:Hodgkin Disease / Q000276:immunology; D006801:Humans; D016130:Immunophenotyping / Q000379:methods*; D007223:Infant; D015470:Leukemia, Myeloid, Acute / Q000276:immunology; D008228:Lymphoma, Non-Hodgkin / Q000276:immunology*; D008297:Male; D009369:Neoplasms / Q000473:pathology*; D009447:Neuroblastoma / Q000276:immunology; D010641:Phenotype; D015203:Reproducibility of Results; D012175:Retinoblastoma / Q000276:immunology; D012189:Retrospective Studies; D016896:Treatment Outcome