title: Increased levels of deleted in malignant brain tumours 1 (DMBT1) in active bacteria-related appendicitis.
abstract: OBJECTIVE
  Deleted in malignant brain tumours 1 (DMBT1; gp340) is a secreted glycoprotein which is found in the surface lining epithelia of human small and large intestine. DMBT1 is suggested to play a role in enterocyte differentiation and surface protection from intestinal bacteria. The aim of this study was to elucidate DMBT1 expression in bacteria-related active intestinal inflammation such as appendicitis.
  
  
  RESULTS
  mRNA and protein levels of DMBT1 were analysed in surgical resections of 50 appendices (active inflammation: n = 25). In non-actively inflamed appendices, inter-individual differences in basal DMBT1 levels of enterocytes and some non-epithelial cells were found. In active appendicitis, enterocytic DMBT1 mRNA expression was increased approximately fivefold, which was paralleled by a corresponding increase of cytoplasmic and secreted DMBT1 protein levels. Increased DMBT1 expression was predominant in enterocytes adjacent to erosive lesions or ulcers.
  
  
  CONCLUSIONS
  Our data demonstrate that bacteria-related active inflammation results in a sharp increase of DMBT1 levels in enterocytes. These findings substantiate the view that DMBT1 is of functional relevance for host defence and modulation of the course of intestinal bacteria-related inflammatory responses.
authors.affiliation: Department of Pediatrics Institute of Pathology, RWTH Aachen University, Aachen, Germany.
authors.forename: Elke
authors.initials: E
authors.lastname: Kaemmerer
authors.forename: Ursula
authors.initials: U
authors.lastname: Schneider
authors.forename: Christina
authors.initials: C
authors.lastname: Klaus
authors.forename: Patrick
authors.initials: P
authors.lastname: Plum
authors.forename: Andrea
authors.initials: A
authors.lastname: Reinartz
authors.forename: Maximilian
authors.initials: M
authors.lastname: Adolf
authors.forename: Marcus
authors.initials: M
authors.lastname: Renner
authors.forename: Tim G A M
authors.initials: TG
authors.lastname: Wolfs
authors.forename: Boris W
authors.initials: BW
authors.lastname: Kramer
authors.forename: Norbert
authors.initials: N
authors.lastname: Wagner
authors.forename: Jan
authors.initials: J
authors.lastname: Mollenhauer
authors.forename: Nikolaus
authors.initials: N
authors.lastname: Gassler
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001064:Appendicitis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D001065:Appendix / Q000378:metabolism* / Q000473:pathology; D002135:Calcium-Binding Proteins; D002648:Child; D004268:DNA-Binding Proteins; D020895:Enterocytes / Q000378:metabolism* / Q000473:pathology; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D025521:Tumor Suppressor Proteins
chemical_list: D002135:Calcium-Binding Proteins; C108174:DMBT1 protein, human; D004268:DNA-Binding Proteins; D011956:Receptors, Cell Surface; D025521:Tumor Suppressor Proteins