title: Surface immobilization of MEPE peptide onto HA/β-TCP ceramic particles enhances bone regeneration and remodeling.
abstract: Calcium phosphate ceramics have been widely used as scaffolds for bone regeneration. Here, to improve the osteogenic potential of hydroxyapatite/β-tricalcium phosphate (HA/β-TCP) and to apply the bioactive peptide in situ, matrix extracellular phosphoglycoprotein (MEPE) peptide, which has been shown to stimulate osteoblast differentiation, was covalently and directionally immobilized on HA/β-TCP particles. The free-hydroxyl groups on the surface of the HA/β-TCP particles were sequentially conjugated with APTES, PEG-(SS)(2), and the synthetic MEPE peptide. Using FTIR and XPS, immobilization of the MEPE peptide on the HA/β-TCP was confirmed. Implantation of the MEPE peptide-immobilized HA/β-TCP into calvarial defect and subsequent analyses using a micro CT and histology showed significant bone regeneration and increased bone area (9.89-fold) as compared to that of unmodified HA/β-TCP. Moreover, tartrate-resistant acid phosphatase-positive osteoclasts were observed in regenerated bone by the MEPE peptide-immobilized HA/β-TCP, indicating that the bones newly formed by the MEPE peptide-immobilized HA/β-TCP are actively remodeled by osteoclasts. Therefore, our data demonstrate that MEPE peptide immobilization onto the HA/β-TCP surface stimulates bone regeneration associated with physiological bone remodeling.
authors.affiliation: Department of Pathology and Regenerative Medicine, School of Dentistry, Institute for Hard Tissue and Bio-Tooth Regeneration, Kyungpook National University, Daegu, Korea.
authors.forename: Bodhraj
authors.initials: B
authors.lastname: Acharya
authors.forename: So-Young
authors.initials: SY
authors.lastname: Chun
authors.forename: Shin-Yoon
authors.initials: SY
authors.lastname: Kim
authors.forename: Cheil
authors.initials: C
authors.lastname: Moon
authors.forename: Hong-In
authors.initials: HI
authors.lastname: Shin
authors.forename: Eui Kyun
authors.initials: EK
authors.lastname: Park
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D002130:Calcium Phosphates / Q000737:chemistry / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D004195:Disease Models, Animal; D017886:Durapatite / Q000737:chemistry / Q000494:pharmacology; D016326:Extracellular Matrix Proteins / Q000737:chemistry / Q000494:pharmacology; D006023:Glycoproteins / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry / Q000494:pharmacology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010006:Osteoblasts / Q000378:metabolism / Q000473:pathology; D010010:Osteoclasts / Q000378:metabolism / Q000473:pathology; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology; D010750:Phosphoproteins / Q000737:chemistry / Q000494:pharmacology; D012887:Skull Fractures / Q000378:metabolism / Q000473:pathology / Q000628:therapy*
chemical_list: D002130:Calcium Phosphates; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D055767:Immobilized Proteins; C486579:MEPE protein, human; D010455:Peptides; D010750:Phosphoproteins; C485817:beta-tricalcium phosphate; D017886:Durapatite