title: Simplifying native chemical ligation with an N-acylsulfonamide linker.
abstract: We report a simplified procedure for the chemical ligation of peptides by using the sulfamylbutyryl linker as a mildly activating group capable of participating in ligation. When the peptidyl N-methylsulfonamide is directly added with excess thiols to ligation reactions, the speed of reaction is comparable to native chemical ligation.
authors.affiliation: UPMC Univ Paris 06, CNRS, ENS, UMR 7203 Laboratoire des Biomolécules, 4 place Jussieu, 75005 Paris, France.
authors.forename: Fabienne
authors.initials: F
authors.lastname: Burlina
authors.forename: Caroline
authors.initials: C
authors.lastname: Morris
authors.forename: Raymond
authors.initials: R
authors.lastname: Behrendt
authors.forename: Peter
authors.initials: P
authors.lastname: White
authors.forename: John
authors.initials: J
authors.lastname: Offer
mesh_terms: D002623:Chemistry Techniques, Analytical; D002851:Chromatography, High Pressure Liquid; D015394:Molecular Structure; D010455:Peptides / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013449:Sulfonamides / Q000737:chemistry*
chemical_list: D010455:Peptides; D013438:Sulfhydryl Compounds; D013449:Sulfonamides