title: [Determination of virginiamycin M1, in feeds by ultra high performance liquid chromatography-tandem mass spectrometry].
abstract: A comprehensive analytical method based on ultra high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) has been developed for the determination of virginiamycin M1 in feeds. The sample was extracted twice by ultrasonic extraction with ace-tonitrile-0.2% (v/v) formic acid (8:2, v/v). The chromatographic separation was achieved with a BEH C18 column and acetonitrile-0. 3% (v/v) formic acid (35: 65, v/v) as the mobile phase. The identification and quantification of the analyte were carried out on electrospray ionization MS/MS in a multiple reaction monitoring (MRM) mode. The correlation coefficient (r) of virginiamycin M1 was 0. 999 5 in the linear range of 0. 3 -226. 6 microg/L. The detection limit (S/ N = 3) and quantification limit (S/N = 10) of virginiamycin M1 were 2 microg/kg and 7 microg/kg, respectively. The average spiked recoveries were in the range of 82. 6% to 102. 7% with the relative standard deviations (RSDs) of 0.9% - 10.5%. The results demonstrate that the proposed method is simple, sensitive, repeatable and suitable for the testing of virginiamycin M, in feeds.
authors.affiliation: National Quality Supervision and Testing Center for Processed Food, Fujian Inspection and Research Institute for Product Quality, Fuzhou 350002, China. huangyonghui1982@163.com
authors.forename: Yonghui
authors.initials: Y
authors.lastname: Huang
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005506:Food Contamination / Q000517:prevention & control*; D025364:Streptogramin A / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents; D025364:Streptogramin A