title: Differential fucosyltransferase IV expression in squamous carcinoma cells is regulated by promoter methylation.
abstract: Enhanced fucosyltransferase IV (FUT4) expression correlates with increased tumor malignancy in many carcinomas. However, little is known about the regulation of FUT4 expression, and whether FUT4 expression is influenced by the methylation status of the FUT4 promoter is unclear. In this study, we demonstrated that FUT4 expression is negatively correlated with the methylation degree of a CpG island in the FUT4 promoter, suggesting that the methylation status of FUT4 promoter regulates the expression of FUT4. The results indicate that manipulating the methylation status of the FUT4 promoter to regulate FUT4 expression may be a novel approach in the treatment of malignant tumors.
authors.affiliation: Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian, Liaoning, People's Republic of China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Li
authors.forename: Shaoming
authors.initials: S
authors.lastname: Tong
authors.forename: Jiwei
authors.initials: J
authors.lastname: Liu
authors.forename: Li
authors.initials: L
authors.lastname: Han
authors.forename: Xuesong
authors.initials: X
authors.lastname: Yang
authors.forename: Hesheng
authors.initials: H
authors.lastname: Hou
authors.forename: Qiu
authors.initials: Q
authors.lastname: Yan
authors.forename: Xiao-Qi
authors.initials: XQ
authors.lastname: Wang
mesh_terms: D001374:Azacitidine / Q000031:analogs & derivatives / Q000494:pharmacology; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D018899:CpG Islands / Q000235:genetics; D003570:Cytidine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D019175:DNA Methylation / Q000187:drug effects; D005647:Fucosyltransferases / Q000235:genetics / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D016256:Lewis X Antigen / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic
chemical_list: D016256:Lewis X Antigen; D003570:Cytidine Triphosphate; C038673:5-aza-2'-deoxycytidine-5'-triphosphate; C066693:FUT4 protein, human; D005647:Fucosyltransferases; D001374:Azacitidine