title: Development of the human abducens nucleus: a morphometric study.
abstract: BACKGROUND
  The abducens nucleus directly innervates the lateral rectus muscle and plays a role in controlling conjugate horizontal eye movements. Although the neuronal cytoarchitecture of the abducens nucleus has been extensively investigated in various species of vertebrates, few studies have been undertaken in humans, especially in fetuses or neonates.
  
  
  METHODS
  We examined 12 human brains from preterm infants aged 20-43 postmenstrual weeks to document the histology and morphometry of the abducens nucleus. The brain was processed into celloidin-embedded serial sections stained with the Klüver-Barrera and other conventional methods.
  
  
  RESULTS
  The nucleus was identified as a mass of cells as early as 20 weeks. Its neurons were clearly distinguished from glial cells due to droplet-like, clear nuclei containing prominent nucleoli and surrounded by a basophilic perikaryon. Neurons of various sizes and shapes were intermingled within the nucleus, although larger neurons were located towards the center of the nucleus. Immature granular or reticular Nissl bodies were seen at 20-21 weeks. Tigroid, coarse Nissl bodies appeared around 28-29 weeks in larger neurons, although in smaller neurons Nissl bodies were dispersed or concentrated peripherally. Morphometric results were: (1) the nuclear volume exponentially increased with age between 20 and 43 weeks; (2) the histograms of neuronal profile areas showed a non-normal distribution trailing toward the right and widening with age; (3) the geometric average of neuronal profile areas increased linearly with age.
  
  
  CONCLUSIONS
  Our study suggests that the human abducens nucleus enlarges more quickly toward the end of gestation, and comprises heterogeneous groups of neurons.
authors.affiliation: Department of Pathology, Dokkyo University School of Medicine, Tochigi, Japan. katsuyukiy@cc9.ne.jp
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Yamaguchi
authors.forename: Koichi
authors.initials: K
authors.lastname: Honma
mesh_terms: D000010:Abducens Nerve / Q000254:growth & development*; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007234:Infant, Premature; D008297:Male; D009474:Neurons / Q000166:cytology*; D011149:Pons / Q000166:cytology* / Q000254:growth & development*