title: Observation of T-2 toxin and HT-2 toxin glucosides from Fusarium sporotrichioides by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS).
abstract: The trichothecenes produced by solid and liquid cultures of Fusarium sporotrichioides were evaluated with high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS). Along with the expected T-2 toxin HT-2 toxin and neosolaniol, two additional compounds were detected, which had ions 162 m/z higher than those in the mass spectra of T-2 toxin or HT-2 toxin. Fragmentation behavior of these two compounds was similar to that of T-2 toxin and HT-2 toxin. Based on LC-MS/MS behavior, it is proposed that the two compounds are T-2 toxin 3-O-glucoside and HT-2 toxin 3-O-glucoside. Production of the two glucosides was measured in kernels from wheat and oat inoculated with F. sporotrichiodes, as well as in cultures grown in liquid media and on cracked corn or rice. Production of glucosides in wheat and oats suggest that they may also be present in naturally contaminated cereals.
authors.affiliation: U.S. Agricultural Research Service, National Center for Agricultural Utilization Department of Agriculture Research, Peoria, IL 61604, USA. Mark.Busman@ars.usda.gov
authors.forename: Mark
authors.initials: M
authors.lastname: Busman
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Poling
authors.forename: Chris M
authors.initials: CM
authors.lastname: Maragos
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002523:Edible Grain / Q000382:microbiology; D005670:Fusarium / Q000378:metabolism*; D005960:Glucosides / Q000032:analysis* / Q000096:biosynthesis; D013605:T-2 Toxin / Q000031:analogs & derivatives* / Q000032:analysis* / Q000096:biosynthesis; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D005960:Glucosides; D013605:T-2 Toxin; C012351:HT-2 toxin