title: Evaluation of the anti-inflammatory effect of milk fermented by a strain of IL-10-producing Lactococcus lactis using a murine model of Crohn's disease.
abstract: Interleukin-10 (IL-10) is the most important anti-inflammatory cytokine at intestinal level, and its absence is involved in inflammatory bowel diseases. However, oral treatment with IL-10 is difficult because of its low survival in the gastrointestinal tract and systemic treatments lead to undesirable side effects. The aim of this paper was to evaluate the anti-inflammatory effect of the administration of milks fermented by Lactococcus lactis strains that produce IL-10 under the control of the xylose-inducible expression system using a trinitrobenzenesulfonic acid-induced colitis murine model. Mice that received milks fermented by L. lactis strains producing IL-10 in the cytoplasm (Cyt strain) or secreted to the product (Sec strain) showed lower damage scores in their large intestines, decreased IFN-γ levels in their intestinal fluids and lower microbial translocation to liver, compared to mice receiving milk fermented by the wild-type strain or those not receiving any treatment. The results obtained in this study show that the employment of fermented milks as a new form of administration of IL-10-producing L. lactisis effective in the prevention of inflammatory bowel disease in a murine model.
authors.affiliation: Centro de Referencia para Lactobacilos, San Miguel de Tucumán, Argentina.
authors.forename: Silvina
authors.initials: S
authors.lastname: del Carmen
authors.forename: Alejandra
authors.initials: A
authors.lastname: de Moreno de LeBlanc
authors.forename: Gabriela
authors.initials: G
authors.lastname: Perdigon
authors.forename: Vanessa
authors.initials: V
authors.lastname: Bastos Pereira
authors.forename: Anderson
authors.initials: A
authors.lastname: Miyoshi
authors.forename: Vasco
authors.initials: V
authors.lastname: Azevedo
authors.forename: Jean Guy
authors.initials: JG
authors.lastname: LeBlanc
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000032:analysis*; D018988:Bacterial Translocation; D003092:Colitis / Q000139:chemically induced / Q000473:pathology / Q000628:therapy; D003424:Crohn Disease / Q000473:pathology / Q000628:therapy*; D004032:Diet / Q000379:methods*; D004195:Disease Models, Animal; D005243:Feces / Q000737:chemistry; D005285:Fermentation; D007371:Interferon-gamma / Q000032:analysis; D016753:Interleukin-10 / Q000032:analysis*; D013294:Lactococcus lactis / Q000235:genetics / Q000378:metabolism*; D008099:Liver / Q000382:microbiology; D051379:Mice; D008892:Milk / Q000737:chemistry* / Q000382:microbiology; D030781:Organisms, Genetically Modified / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000032:analysis; D012720:Severity of Illness Index
chemical_list: D000893:Anti-Inflammatory Agents; D011994:Recombinant Proteins; D016753:Interleukin-10; D007371:Interferon-gamma