title: Molecular characterization, phylogenetic analysis and expression patterns of five protein arginine methyltransferase genes of channel catfish, Ictalurus punctatus (Rafinesque).
abstract: Protein arginine methylation, catalyzed by protein arginine methyltransferases (PRMT), has recently emerged as an important modification in the regulation of gene expression. In this communication, we identified and characterized the channel catfish orthologs to human PRMT 1, 3, 4 and 5, and PRMT4 like. Each PRMT nucleic acid sequence has an open reading frame (ORF) and 3'-untranslated regions. Each ORF appears to encode 361, 587 and 458 amino acid residues for PRMT1, PRMT4 and variant, respectively. The partial ORF of PRMT3 and PRMT5 encode 292 and 563 amino acids, respectively. By comparison with the human counterparts, each channel catfish PRMT also has conserved domains. For expression profile, the channel catfish PRMT1 transcript was detected by RT-PCR in spleens, anterior kidneys, livers, intestines, skin and gills of fish examined. Except in liver, the PRMT3 transcript was detected in all catfish tissues examined. However, the PRMT4 cDNA was detected in livers from all three catfish and gills from two fish, but not other tissues. This information will enable us to further elucidate PRMT functions in channel catfish.
authors.affiliation: Poultry Microbiological Safety Research Unit, United States Department of Agriculture, Richard B. Russell Agricultural Research Center, Agricultural Research Service, 950 College Station Road, Athens, GA 30605-2720, USA. hungyueh.yeh@ars.usda.gov
authors.forename: Hung-Yueh
authors.initials: HY
authors.lastname: Yeh
authors.forename: Phillip H
authors.initials: PH
authors.lastname: Klesius
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017756:Aquaculture; D002397:Catfishes / Q000235:genetics / Q000378:metabolism*; D020224:Expressed Sequence Tags; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D008969:Molecular Sequence Data; D010802:Phylogeny; D011484:Protein-Arginine N-Methyltransferases / Q000235:genetics / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D029941:Fish Proteins; D011484:Protein-Arginine N-Methyltransferases