title: Detection and quantification of monomers in unstimulated whole saliva after treatment with resin-based composite fillings in vivo.
abstract: Resin-based dental restorative materials contain allergenic methacrylate monomers, which may be released into saliva after restorative treatment. Monomers from resin-based composite materials have been demonstrated in saliva in vitro; however, studies analyzing saliva after restorative therapy are scarce. The aim of this study was to quantify methacrylate monomers in saliva after treatment with a resin-based composite filling material. Saliva was collected from 10 patients at four start points--before treatment, and 10 min, 24 h, and 7 d after treatment--and analysed by combined chromatography/mass spectrometry. The monomers bisphenol-A diglycidyl methacrylate (Bis-GMA), 2-hydroxyethyl methacrylate (HEMA), and urethane dimethacrylate (UDMA) were detected and quantified in the samples collected shortly (10 min) after treatment. The amounts detected ranged from 0.028 to 9.65 μg ml(-1) for Bis-GMA, from 0.015 to 0.19 μg ml(-1) for HEMA, and from 0.004 to 1.2 μg ml(-1) for UDMA. Triethyleneglycol dimethacrylate (TEGDMA) was detected in four of the samples. Ethoxylated bisphenol-A dimethacrylate (Bis-EMA) was not detected. Monomers were not detected in saliva samples collected before treatment, or 24 h or 7 d after treatment, with the exception of one sample, 24 h after treatment, in which HEMA was detected. In conclusion, monomers from the investigated resin-based composite and adhesive system were present in saliva shortly after treatment. One week after treatment, no monomers could be detected in patients' saliva samples.
authors.affiliation: Department of Clinical Dentistry, University of Tromsø, Tromsø, Norway. vibeke.michelsen@odont.uib.no
authors.forename: Vibeke B
authors.initials: VB
authors.lastname: Michelsen
authors.forename: Hilde B M
authors.initials: HB
authors.lastname: Kopperud
authors.forename: Gunvor B
authors.initials: GB
authors.lastname: Lygre
authors.forename: Lars
authors.initials: L
authors.lastname: Björkman
authors.forename: Einar
authors.initials: E
authors.lastname: Jensen
authors.forename: Inger S
authors.initials: IS
authors.lastname: Kleven
authors.forename: Johanna
authors.initials: J
authors.lastname: Svahn
authors.forename: Henning
authors.initials: H
authors.lastname: Lygre
mesh_terms: D017438:Bisphenol A-Glycidyl Methacrylate / Q000032:analysis / Q000737:chemistry; D003188:Composite Resins / Q000032:analysis / Q000737:chemistry*; D003737:Dental Cavity Preparation / Q000145:classification; D003764:Dental Materials / Q000032:analysis / Q000737:chemistry*; D003793:Dental Restoration, Permanent / Q000145:classification; D017220:Dentin-Bonding Agents / Q000032:analysis / Q000737:chemistry; D005260:Female; D005500:Follow-Up Studies; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008297:Male; D008689:Methacrylates / Q000032:analysis* / Q000737:chemistry; D008875:Middle Aged; D011092:Polyethylene Glycols / Q000032:analysis / Q000737:chemistry; D011109:Polymethacrylic Acids / Q000032:analysis / Q000737:chemistry; D011140:Polyurethanes / Q000032:analysis / Q000737:chemistry; D019279:Resin Cements / Q000032:analysis / Q000737:chemistry; D012463:Saliva / Q000737:chemistry*
chemical_list: D003188:Composite Resins; D003764:Dental Materials; D017220:Dentin-Bonding Agents; C444701:Filtek Z250; D008689:Methacrylates; D011109:Polymethacrylic Acids; D011140:Polyurethanes; D019279:Resin Cements; C029824:urethane dimethacrylate luting resin; C020946:triethylene glycol dimethacrylate; C041979:2,2-bis-(4-(2-methacryloxyethoxy)phenyl)propane; D011092:Polyethylene Glycols; D017438:Bisphenol A-Glycidyl Methacrylate; C005044:hydroxyethyl methacrylate; C041330:Scotchbond