title: Capillary electrophoresis with Orbitrap-Velos mass spectrometry detection.
abstract: Capillary electrophoresis can provide fast and efficient separations of peptides. However, the high speed separation and limited loading capacity of capillary electrophoresis requires the use of a fast and sensitive detector. While laser-induced fluorescence provides exquisite sensitivity and millisecond response time, it inherently generates a low information content signal. In contrast, mass spectrometry provides an information rich signal that is attractive for peptide analysis. The recently introduced Velos-Orbitrap mass spectrometer is capable of fast and sensitive tandem MS acquisition and simultaneous high accuracy MS acquisition, which is well suited for coupling with fast and efficient separation methods for peptide analysis. We evaluated this instrument as a detector for peptide separation by capillary electrophoresis. In MS mode, we observed low attomole detection limits for a number of peptides in a tryptic digest of standard proteins with high mass resolution (30,000 at m/z 400). The response time of the Orbitrap at this resolution was ∼0.70s, which was adequate to reconstruct the peak shape and area of our electrophoretic peaks. The linear ion-trap successfully recorded tandem MS spectra of tryptic peptides at 20 nM concentration.
authors.affiliation: Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556, United States.
authors.forename: Roza
authors.initials: R
authors.lastname: Wojcik
authors.forename: Yihan
authors.initials: Y
authors.lastname: Li
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Maccoss
authors.forename: Norman J
authors.initials: NJ
authors.lastname: Dovichi
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis*; D012441:Saccharomyces cerevisiae / Q000737:chemistry; D029701:Saccharomyces cerevisiae Proteins / Q000032:analysis*; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013997:Time Factors
chemical_list: D010455:Peptides; D029701:Saccharomyces cerevisiae Proteins