title: Response of monocyte-derived dendritic cells to rapidly solidified nickel-titanium ribbons with shape memory properties.
abstract: Ni-Ti Shape Memory Alloys (SMAs) have attracted considerable attention as biomaterials for medical devices. However, the biocompatibility of Ni-Ti SMAs is often unsatisfactory due to their poor surface structure. Here we prepared Rapidly Solidified (RS) Ni-Ti SMA ribbons by melt-spinning and their surface was characterised by Auger-electron spectroscopy, X-ray photoelectron spectrometry and scanning electron microscopy. The biocompatibility of the produced ribbons and their immunomodulatory properties were studied on human monocyte-derived dendritic cells (MoDCs). We showed that melt-spinning of Ni-Ti SMAs can form a thin homogenous oxide layer, which improves their corrosion resistance and subsequent toxicity to MoDCs. Ni-Ti RS ribbons stimulated the maturation of MoDCs, as detected by changes in the cells' morphology and increased expression of HLA-DR, CD86, CD40 and CD83 molecules. However, Ni-Ti RS ribbons enhanced the tolerogenic properties of immature MoDCs, which produced higher levels of IL-10 and IL-27, driving the differentiation of IL-10- and TGF-β-producing CD4+T cells. On the other hand, in the presence of lipopolysaccharide, an important pro-inflammatory biomolecule, Ni-Ti RS ribbons enhanced the allostimulatory and Th1 polarising capacity of MoDCs, whereas the production of Th2 and Th17 cytokines was down-regulated. In conclusion, Ni-Ti RS ribbons possess substantial immunomodulatory properties on MoDCs. These findings might be clinically relevant, because implanted Ni-Ti SMA devices can induce both desired and adverse effects on the immune system, depending on the microenvironmental stimuli.
authors.affiliation: Military Medical Academy, Crnotravska 17, 11000 Belgrade, Serbia. fakultet.vma@mod.gov.rs
authors.forename: S
authors.initials: S
authors.lastname: Tomić
authors.forename: R
authors.initials: R
authors.lastname: Rudolf
authors.forename: M
authors.initials: M
authors.lastname: Brunčko
authors.forename: I
authors.initials: I
authors.lastname: Anžel
authors.forename: V
authors.initials: V
authors.lastname: Savić
authors.forename: M
authors.initials: M
authors.lastname: Colić
mesh_terms: D015703:Antigens, CD / Q000187:drug effects / Q000378:metabolism*; D001672:Biocompatible Materials / Q000494:pharmacology*; D002470:Cell Survival; D002478:Cells, Cultured; D016207:Cytokines / Q000187:drug effects / Q000378:metabolism*; D003713:Dendritic Cells / Q000166:cytology* / Q000187:drug effects; D006684:HLA-DR Antigens / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D056747:Immunomodulation; D009000:Monocytes / Q000166:cytology* / Q000187:drug effects; D009532:Nickel / Q000494:pharmacology*; D010641:Phenotype; D013499:Surface Properties; D014025:Titanium / Q000494:pharmacology*
chemical_list: D015703:Antigens, CD; D001672:Biocompatible Materials; D016207:Cytokines; D006684:HLA-DR Antigens; C040654:titanium nickelide; D009532:Nickel; D014025:Titanium