title: Validation of a gas chromatography-ion trap-tandem mass spectrometry assay for the simultaneous quantification of cocaine, benzoylecgonine, cocaethylene, morphine, codeine, and 6-acetylmorphine in aqueous solution, blood, and skeletal muscle tissue.
abstract: A gas chromatography-ion trap-tandem mass spectrometry method was developed and validated for the simultaneous solid-phase extraction and quantification of cocaine, benzoylecgonine, cocaethylene, morphine, codeine, and 6-monoacteylmorphine in blood, muscle tissue, and water. An assay for total morphine in blood and muscle was also validated. The limit of quantification was ≤ 0.01 mg/kg in muscle and ≤ 0.005 mg/L in blood and water. Good linearity was observed over the concentration ranges studied (r ≥ 0.99). The repeatability (% RSD) at the three concentration levels was typically ≤ 15% and never exceeded 17%. Intermediate precision of ≤ 16% was obtained for all matrices. Deviation < 20% of the nominal concentration was obtained in all repeatability and intermediate precision experiments. Extraction recoveries for cocaine and metabolites ranged from 91% to 110% in water, 81% to 110% in blood, and 61% to 75% in muscle. Recoveries for opiates ranged from 59% to 104% in water, 50% to 95% in blood, and 41% to 79% in muscle. The hydrolysis efficiency for the total morphine assay in blood and muscle ranged from 91% to 99% with within-day and intermediate precisions of ≤ 14% and ≤ 12%, respectively.
authors.affiliation: Department of Forensic and Biological Sciences, Bournemouth University, Talbot Campus, Fern Barrow, Poole, Dorset, United Kingdom.
authors.forename: Kelly A
authors.initials: KA
authors.lastname: Rees
authors.forename: Poppy A
authors.initials: PA
authors.lastname: McLaughlin
authors.forename: M David
authors.initials: MD
authors.lastname: Osselton
mesh_terms: D000818:Animals; D003042:Cocaine / Q000031:analogs & derivatives* / Q000032:analysis*; D003061:Codeine / Q000032:analysis*; D003670:Deer; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006736:Horses; D009020:Morphine / Q000032:analysis*; D009022:Morphine Derivatives / Q000032:analysis*; D018482:Muscle, Skeletal / Q000737:chemistry*; D012996:Solutions; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D009022:Morphine Derivatives; D012996:Solutions; C005618:benzoylecgonine; D009020:Morphine; C066444:cocaethylene; D003042:Cocaine; C026979:6-O-monoacetylmorphine; D003061:Codeine