title: Construction and characterization of a gradually inducible expression vector for Halobacterium salinarum, based on the kdp promoter.
abstract: Gradually inducible expression vectors which are governed by variations of growth conditions are powerful tools for gene expression of conditionally lethal mutants. Furthermore, controlled expression allows monitoring of overproduction of proteins at various stages in their expressing hosts. For Halobacterium salinarum, which is often used as a paradigm for halophilic archaea, such an inducible expression system is not available to date. Here we show that the kdp promoter (Pkdp), which facilitates gene expression upon K(+) limitation, can be used to establish such a system for molecular applications. Pkdp features a rather high expression rate, with an approximately 50-fold increase that can be easily varied by K(+) concentrations in the growth medium. Besides the construction of an expression vector, our work describes the characterization of expression patterns and, thus, offers a gradually inducible expression system to the scientific community.
authors.affiliation: Universität Osnabrück, Fachbereich Biologie/Chemie, Osnabrück, Germany.
authors.forename: Dorthe
authors.initials: D
authors.lastname: Kixmüller
authors.forename: Jörg-Christian
authors.initials: JC
authors.lastname: Greie
mesh_terms: D019843:Archaeal Proteins / Q000235:genetics / Q000378:metabolism*; D003470:Culture Media; D019641:DNA, Archaeal / Q000235:genetics; D019848:Gene Expression Regulation, Archaeal; D019766:Genes, Archaeal; D005822:Genetic Vectors; D019617:Halobacterium salinarum / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D009876:Operon; D011188:Potassium / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D019843:Archaeal Proteins; D003470:Culture Media; D019641:DNA, Archaeal; D001616:beta-Galactosidase; D011188:Potassium