title: Determination of the absolute configuration of peptide natural products by using stable isotope labeling and mass spectrometry.
abstract: Structure elucidation of natural products including the absolute configuration is a complex task that involves different analytical methods like mass spectrometry, NMR spectroscopy, and chemical derivation, which are usually performed after the isolation of the compound of interest. Here, a combination of stable isotope labeling of Photorhabdus and Xenorhabdus strains and their transaminase mutants followed by detailed MS analysis enabled the structure elucidation of novel cyclopeptides named GameXPeptides including their absolute configuration in crude extracts without their actual isolation.
authors.affiliation: Institut für Molekulare Biowissenschaften, Molekulare Biotechnologie, Goethe Universität Frankfurt, Max-von-Laue-Strasse 9, 60436 Frankfurt am Main, Germany. h.bode@bio.uni-frankfurt.de
authors.forename: Helge B
authors.initials: HB
authors.lastname: Bode
authors.forename: Daniela
authors.initials: D
authors.lastname: Reimer
authors.forename: Sebastian W
authors.initials: SW
authors.lastname: Fuchs
authors.forename: Ferdinand
authors.initials: F
authors.lastname: Kirchner
authors.forename: Christina
authors.initials: C
authors.lastname: Dauth
authors.forename: Carsten
authors.initials: C
authors.lastname: Kegler
authors.forename: Wolfram
authors.initials: W
authors.lastname: Lorenzen
authors.forename: Alexander O
authors.initials: AO
authors.lastname: Brachmann
authors.forename: Peter
authors.initials: P
authors.lastname: Grün
mesh_terms: D001688:Biological Products / Q000737:chemistry*; D007553:Isotope Labeling / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry / Q000379:methods*; D010455:Peptides / Q000737:chemistry*; D010456:Peptides, Cyclic / Q000737:chemistry*; D013237:Stereoisomerism
chemical_list: D001688:Biological Products; D010455:Peptides; D010456:Peptides, Cyclic