title: Differences between sympatric populations of Eotetranychus carpini collected from Vitis vinifera and Carpinus betulus: insights from host-switch experiments and molecular data.
abstract: Eotetranychus carpini (Oudemans) is an important pest of grapevine (Vitis vinifera L.) in southern Europe. This mite is also found on a number of different plants, including Carpinus betulus L., which commonly occurs in stands and hedgerows bordering vineyards, where it may serve as a potential mite reservoir. The economic importance of this pest has motivated a number of studies aimed at investigating whether the mites found on V. vinifera and C. betulus are conspecific. The results obtained to date have been inconclusive. In this study, we used biological and molecular approaches to investigate this issue. First, we conducted host-switch experiments to test the ability of E. carpini to develop on an alternative host plant, using mite populations originally collected on either C. betulus or V. vinifera plants from the same area. Second, we investigated DNA-based differentiation using nucleotide sequences of the ITS1-5.8S-ITS2 region of the ribosomal DNA of individual E. carpini from the populations examined in our host-plant experiments. We also analyzed sequences of individuals collected in other regions (Italy and Slovenia) to estimate species variation. The results from our host-switch experiments suggest the differentiation of mites collected on the two hosts. Mites collected from C. betulus did not survive and reproduce on V. vinifera and vice versa. Our molecular work revealed significant genetic differentiation between the mites collected from the two hosts, but no evidence of genetic variation among specimens collected from the same host species. Our results indicate the existence of host races of E. carpini.
authors.affiliation: Unità di Protezione delle Piante e Biodiversità Agroforestale, Fondazione Edmund Mach, Technology Transfer Centre, Via E. Mach, 1, 38010 San Michele all'Adige, Trento, Italy. valeria.malagnini@iasma.it
authors.forename: Valeria
authors.initials: V
authors.lastname: Malagnini
authors.forename: Maria
authors.initials: M
authors.lastname: Navajas
authors.forename: Alain
authors.initials: A
authors.lastname: Migeon
authors.forename: Carlo
authors.initials: C
authors.lastname: Duso
mesh_terms: D000818:Animals; D001483:Base Sequence; D029401:Betulaceae; D005247:Feeding Behavior; D005260:Female; D007558:Italy; D008018:Life Cycle Stages; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D040102:Tetranychidae / Q000235:genetics / Q000502:physiology*; D027843:Vitis