title: Induction of mutation to ouabain resistance and of malignant transformation by chemicals in cloned mouse M2 fibroblasts.
abstract: Malignant transformation and mutation to ouabain-resistance is induced in cloned M2 mouse fibroblasts in vitro by treatment with N-methyl-N'-nitro-N-nitrosoguanidine and two derivatives of polycyclic aromatic hydrocarbons, the 3,4-diol derived from 7-methylbenz [a]anthracene and the 7,8-diol-9,10-oxide derived from benzo[a]pyrene. Transformation is an event more frequent than mutation, the ratio of transformation to mutation frequencies varied between 24 and 1118. Another hydrocarbon derivative, the 9,10-diol-7,8-oxide derived from benzo[a]pyrene, did induce ouabain-resistance without causing transformation. Induced ouabain-resistant clones did not behave like transformants, and transformants did not exhibit ouabain resistance. The significance of these findings is discussed.
authors.affiliation: Department of Toxicology, University of Hamburg Medical School, Martinistrasse 52, 2000 Hamburg 20, GFR.
authors.forename: H
authors.initials: H
authors.lastname: Marquardt
mesh_terms: D000818:Animals; D001551:Benz(a)Anthracenes / Q000633:toxicity; D001580:Benzopyrenes / Q000633:toxicity; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced*; D002478:Cells, Cultured; D004351:Drug Resistance / Q000187:drug effects* / Q000235:genetics*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D008297:Male; D008769:Methylnitronitrosoguanidine / Q000633:toxicity; D051379:Mice; D008809:Mice, Inbred C3H; D009153:Mutagens / Q000633:toxicity*; D009154:Mutation / Q000187:drug effects*; D059645:Mutation Rate; D010042:Ouabain / Q000494:pharmacology*
chemical_list: D001551:Benz(a)Anthracenes; D001580:Benzopyrenes; D009153:Mutagens; D008769:Methylnitronitrosoguanidine; D010042:Ouabain; C021219:7-methylbenzanthracene