title: HBXIP upregulates CD46, CD55 and CD59 through ERK1/2/NF-κB signaling to protect breast cancer cells from complement attack.
abstract: Hepatitis B X-interacting protein (HBXIP) is able to enhance migration of breast cancer cells. However, the role of HBXIP in regulation of complement-dependent cytotoxicity (CDC) in breast cancer is not understood. Here, we report that HBXIP contributes to protecting breast cancer cells from CDC by upregulating membrane-bound complement regulatory protein (mCRPs), including CD46, CD55 and CD59. We found that HBXIP upregulated mCRPs through activating p-ERK1/2/NF-κB. Interestingly, the knockdown of CD59 was able to block the HBXIP-enhanced breast tumor growth in animal. Thus, we conclude that HBXIP upregulates CD46, CD55 and CD59 through p-ERK1/2/NF-κB signaling to protect breast cancer from CDC.
authors.affiliation: Department of Biochemistry, College of Life Sciences, State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300071, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Cui
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhao
authors.forename: Changliang
authors.initials: C
authors.lastname: Shan
authors.forename: Guangyao
authors.initials: G
authors.lastname: Kong
authors.forename: Nan
authors.initials: N
authors.lastname: Hu
authors.forename: Yiwen
authors.initials: Y
authors.lastname: Zhang
authors.forename: Shuai
authors.initials: S
authors.lastname: Zhang
authors.forename: Weiying
authors.initials: W
authors.lastname: Zhang
authors.forename: Yingyi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zhang
authors.forename: Lihong
authors.initials: L
authors.lastname: Ye
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000276:immunology*; D000818:Animals; D001943:Breast Neoplasms / Q000276:immunology*; D018958:CD55 Antigens / Q000276:immunology*; D018957:CD59 Antigens / Q000276:immunology*; D003167:Complement Activation / Q000276:immunology; D003165:Complement System Proteins / Q000276:immunology*; D005260:Female; D006801:Humans; D020935:MAP Kinase Signaling System / Q000276:immunology*; D051916:Membrane Cofactor Protein / Q000276:immunology*; D051379:Mice; D008819:Mice, Nude; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D016328:NF-kappa B / Q000276:immunology*; D015534:Trans-Activators / Q000276:immunology; D014407:Tumor Cells, Cultured; D054334:Viral Regulatory and Accessory Proteins
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D018958:CD55 Antigens; D018957:CD59 Antigens; C110719:LAMTOR5 protein, human; D051916:Membrane Cofactor Protein; D016328:NF-kappa B; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein; D003165:Complement System Proteins; C535150:MAPK1 protein, human; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3