title: A novel sensitive DNA-biosensor for detection of a carcinogen, Sudan II, using electrochemically treated pencil graphite electrode by voltammetric methods.
abstract: A simple and inexpensive methodology was used to develop a novel electrochemical sensor for the determination of Sudan II. The interaction of Sudan II with salmon sperm ds-DNA on the surface of salmon sperm ds-DNA-modified pencil graphite electrode (PGE) and in solution phase was studied, using differential pulse voltammetry. The difference between adenine and guanine signals of the ds-DNA after and before interaction with Sudan II was directly proportional to Sudan II concentration, which used for quantitative inspections. Using PGE, a linear calibration curve (R(2)=0.9958) was observed with 0.5-6.0 μg mL(-1) Sudan II. Furthermore, the LOD of 0.4 μg mL(-1) and linear range between 0.5 and 4.0 μg mL(-1) were achieved in solution phase. In the second part, Sudan II was determined on a pretreated pencil graphite electrode by means of adsorptive stripping differential pulse voltammetry. The peak current was linearly dependent on Sudan II concentration over the range of 0.0015-0.30 μg mL(-1), with a detection limit of 0.00007 μg mL(-1) Sudan II. Both ds-DNA-modified PGE and PPGE were applied to analyze Sudan II in real samples.
authors.affiliation: Department of Chemistry, Isfahan University of Technology, Isfahan 84156-83111, Iran. Ensafi@cc.iut.ac.ir
authors.forename: Ali A
authors.initials: AA
authors.lastname: Ensafi
authors.forename: Behzad
authors.initials: B
authors.lastname: Rezaei
authors.forename: Maryam
authors.initials: M
authors.lastname: Amini
authors.forename: Esmaeil
authors.initials: E
authors.lastname: Heydari-Bafrooei
mesh_terms: D000225:Adenine / Q000737:chemistry; D000327:Adsorption; D000818:Animals; D001391:Azo Compounds / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods*; D002138:Calibration; D002273:Carcinogens / Q000032:analysis*; D004247:DNA / Q000737:chemistry; D004563:Electrochemistry; D004566:Electrodes; D005506:Food Contamination / Q000032:analysis; D006108:Graphite; D006147:Guanine / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D008297:Male; D010084:Oxidation-Reduction; D012474:Salmon; D012996:Solutions; D013094:Spermatozoa / Q000737:chemistry
chemical_list: D001391:Azo Compounds; D002273:Carcinogens; D012996:Solutions; D006147:Guanine; D006108:Graphite; C033003:sudan red; D004247:DNA; D000225:Adenine