title: Progesterone receptor is involved in 2,3,7,8-tetrachlorodibenzo-p-dioxin-stimulated breast cancer cells proliferation.
abstract: Sex hormones are pivotal for both normal and neoplastic development of breast tissues. TCDD, with sex hormonal activity, executes multiple biological activities primarily through AhR. However, the detailed mechanisms how TCDD affects human breast cell are mostly unexplored. We analyzed the biological effects and underlying mechanisms of TCDD on MCF-7 cells. PR, other than AhR, was involved in TCDD-stimulated MCF-7 cell proliferation. TCDD inactivated Akt-FoxO3a pathway, increased cdc25C/cdc2 activity, decreased P21/P27 activity, and enriched G2/M phase, in a PR- and AhR-dependent manner. In conclusion, our study demonstrated for the first time that PR was involved in TCDD-stimulated breast cell proliferation.
authors.affiliation: Department of Biological Science and Technology, I-Shou University, Kaohsiung 824, Taiwan, ROC; Department of Medical Research, E-DA Hospital, I-Shou University, Kaohsiung 824, Taiwan, ROC.
authors.forename: Yun-Ju
authors.initials: YJ
authors.lastname: Chen
authors.affiliation: Department of General Surgery, E-DA Hospital, Kaohsiung 824, Taiwan, ROC.
authors.forename: Chao-Ming
authors.initials: CM
authors.lastname: Hung
authors.affiliation: Department of Obstetrics and Gynecology, E-DA Hospital, Kaohsiung 824, Taiwan, ROC.
authors.forename: Nari
authors.initials: N
authors.lastname: Kay
authors.affiliation: Department of Obstetrics and Gynecology, E-DA Hospital, Kaohsiung 824, Taiwan, ROC.
authors.forename: Chin-Chen
authors.initials: CC
authors.lastname: Chen
authors.affiliation: Department of Medical Research, E-DA Hospital, I-Shou University, Kaohsiung 824, Taiwan, ROC.
authors.forename: Ying-Hsien
authors.initials: YH
authors.lastname: Kao
authors.affiliation: Department of Biological Science and Technology, I-Shou University, Kaohsiung 824, Taiwan, ROC; Department of Obstetrics and Gynecology, E-DA Hospital, Kaohsiung 824, Taiwan, ROC; Department of Medical Research, E-DA Hospital, I-Shou University, Kaohsiung 824, Taiwan, ROC. Electronic address: yuanssf@ms33.hinet.net.
authors.forename: Shyng-Shiou
authors.initials: SS
authors.lastname: Yuan
mesh_terms: D001943:Breast Neoplasms / Q000139:chemically induced / Q000235:genetics* / Q000378:metabolism; D002273:Carcinogens / Q000633:toxicity*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D006801:Humans; D000072317:Polychlorinated Dibenzodioxins / Q000633:toxicity*; D011980:Receptors, Progesterone / Q000235:genetics*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D002273:Carcinogens; D000072317:Polychlorinated Dibenzodioxins; D011980:Receptors, Progesterone