title: Radioligand binding assays for the glycine site on N-methyl-D-aspartate receptors.
abstract: This unit describes a competitive binding assay for the glycine binding site on the NMDA subtype of glutamate receptors in rat brain homogenates. Agonists of the NMDA receptor associated glycine binding site have been proposed as potential therapeutics in cognitive disorders. Conversely, antagonists may be useful in a variety of disorders associated with excessive activation of EAA receptors, including Parkinson, Huntington and Alzheimer Diseases, and neuropathic pain, among others. This unit describes a competitive binding assay for the glycine binding site on the NMDA subtype of glutamate receptors in rat.
authors.affiliation: NovaScreen Bioscience Corporation, Hanover, Maryland, USA.
authors.forename: Scott
authors.initials: S
authors.lastname: Perschke
authors.forename: John
authors.initials: J
authors.lastname: Ferkany
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D005998:Glycine / Q000378:metabolism*; D007211:Indoles / Q000494:pharmacology; D008297:Male; D011869:Radioligand Assay / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D007211:Indoles; C101788:MDL 105519; D016194:Receptors, N-Methyl-D-Aspartate; D005998:Glycine