title: Soluble Toll-like receptor 1 family members in the amniotic fluid of women with preterm prelabor rupture of the membranes.
abstract: OBJECTIVE
  To determine soluble Toll-like receptor (sTLR) 1, sTLR2 and sTLR6 concentrations in amniotic fluid (AF) of women with preterm prelabor rupture of membranes (PPROM) and if there is an association with microbial invasion of the amniotic cavity and histological chorioamnionitis (HCA).
  
  
  METHODS
  Cross-sectional study was performed. Forty-two women with singleton PPROM pregnancies at a gestational age between 24 + 0 and 36 + 6 weeks were included in the study (twenty-two women with presence of both microbial invasion of the amniotic cavity and HCA, and 20 women without microbial invasion of the amniotic cavity and HCA). Amniocenteses were performed, and the concentrations of sTLRs were determined by sandwich enzyme-linked immunosorbent assays.
  
  
  RESULTS
  Women with microbial invasions of the amniotic cavity and HCA (n = 22) had significantly higher median sTLR1, sTLR2 and sTLR6 levels than those without (n = 20). (20.4 ng/mL vs. 0.44 ng/mL; p < 0.0001, 577.6 ng/mL vs. 60.7 ng/mL; p < 0.0001 and 0.44 ng/mL vs. 0.26 ng/mL; p = 0.02, respectively).
  
  
  CONCLUSIONS
  Women with microbial invasion of the amniotic cavity and HCA had higher AF sTLR1, 2 and 6 levels.
authors.affiliation: Department of Obstetrics and Gynecology, Charles University in Prague, Faculty of Medicine Hradec Kralove, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic. kacermar@fnhk.cz
authors.forename: Marian
authors.initials: M
authors.lastname: Kacerovsky
authors.forename: Ctirad
authors.initials: C
authors.lastname: Andrys
authors.forename: Marcela
authors.initials: M
authors.lastname: Drahosova
authors.forename: Ivana
authors.initials: I
authors.lastname: Musilova
authors.forename: Helena
authors.initials: H
authors.lastname: Hornychova
authors.forename: Daniel
authors.initials: D
authors.lastname: Lesko
authors.forename: Jindrich
authors.initials: J
authors.lastname: Tosner
authors.forename: Bo
authors.initials: B
authors.lastname: Jacobsson
mesh_terms: D000328:Adult; D000653:Amniotic Fluid / Q000737:chemistry* / Q000378:metabolism / Q000382:microbiology; D002821:Chorioamnionitis / Q000175:diagnosis / Q000382:microbiology / Q000473:pathology; D003430:Cross-Sectional Studies; D005260:Female; D005322:Premature Rupture of Fetal Membranes / Q000175:diagnosis* / Q000453:epidemiology / Q000378:metabolism; D006801:Humans; D007231:Infant, Newborn; D007235:Infant, Premature, Diseases / Q000175:diagnosis / Q000453:epidemiology; D009017:Morbidity; D005810:Multigene Family; D007752:Obstetric Labor, Premature / Q000175:diagnosis / Q000453:epidemiology / Q000378:metabolism; D011237:Predictive Value of Tests; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods; D011379:Prognosis; D012995:Solubility; D051194:Toll-Like Receptor 1 / Q000032:analysis* / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D055815:Young Adult
chemical_list: D051194:Toll-Like Receptor 1