title: Odorant receptor modulation: ternary paradigm for mode of action of insect repellents.
abstract: The modulation of insect behavior for the purpose of controlling the spread of infectious diseases has been the task of a few insect repellents for which the mechanistic modes of action on odorant receptors (ORs) are unclear. Here, we study the effects of the repellents DEET and IR3535, and a novel OR co-receptor (Orco) agonist on odorant-evoked currents in Xenopus oocytes expressing two subtypes of Aedes aegypti ORs (AaORs). We show that DEET and IR3535 behave as insurmountable antagonists of ORs, and that modulation of OR activity is not restricted to antagonism and agonism, but also includes synergism. This knowledge of the molecular mechanisms underlying OR blockade, activation and hyperactivation will be fundamental to the development of novel strategies for the control of mosquito behavior.
authors.affiliation: United States Department of Agriculture, Agricultural Research Service, Henry A. Wallace Beltsville Agricultural Research Center, Plant Sciences Institute, Invasive Insect Biocontrol and Behavior Laboratory, Beltsville, MD 20705, USA.
authors.forename: Jonathan D
authors.initials: JD
authors.lastname: Bohbot
authors.forename: Joseph C
authors.initials: JC
authors.lastname: Dickens
mesh_terms: D000330:Aedes / Q000187:drug effects*; D000818:Animals; D003671:DEET / Q000494:pharmacology*; D007302:Insect Repellents / Q000494:pharmacology*; D011422:Propionates / Q000494:pharmacology*; D018035:Receptors, Odorant / Q000378:metabolism*
chemical_list: D007302:Insect Repellents; D011422:Propionates; D018035:Receptors, Odorant; D003671:DEET; C462400:ethyl-3-(N-n-butyl-N-acetyl)aminopropionate