title: pH-programmable DNA logic arrays powered by modular DNAzyme libraries.
abstract: Nature performs complex information processing circuits, such the programmed transformations of versatile stem cells into targeted functional cells. Man-made molecular circuits are, however, unable to mimic such sophisticated biomachineries. To reach these goals, it is essential to construct programmable modular components that can be triggered by environmental stimuli to perform different logic circuits. We report on the unprecedented design of artificial pH-programmable DNA logic arrays, constructed by modular libraries of Mg(2+)- and UO(2)(2+)-dependent DNAzyme subunits and their substrates. By the appropriate modular design of the DNA computation units, pH-programmable logic arrays of various complexities are realized, and the arrays can be erased, reused, and/or reprogrammed. Such systems may be implemented in the near future for nanomedical applications by pH-controlled regulation of cellular functions or may be used to control biotransformations stimulated by bacteria.
authors.affiliation: The Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
authors.forename: Johann
authors.initials: J
authors.lastname: Elbaz
authors.forename: Fuan
authors.initials: F
authors.lastname: Wang
authors.forename: Francoise
authors.initials: F
authors.lastname: Remacle
authors.forename: Itamar
authors.initials: I
authors.lastname: Willner
mesh_terms: D039301:Computers, Molecular; D021881:DNA, Catalytic / Q000737:chemistry / Q000378:metabolism*; D030561:Databases, Nucleic Acid; D004867:Equipment Design; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation*; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism; D017974:Uranium Compounds / Q000378:metabolism
chemical_list: D021881:DNA, Catalytic; D021122:Protein Subunits; D017974:Uranium Compounds; D008274:Magnesium; C012597:uranium dioxide