title: Fabrication and application of amperometric glucose biosensor based on a novel PtPd bimetallic nanoparticle decorated multi-walled carbon nanotube catalyst.
abstract: A sensitive, selective and stable amperometric glucose biosensor employing novel PtPd bimetallic nanoparticles decorated on multi-walled carbon nanotubes (PtPd-MWCNTs) was investigated. PtPd-MWCNTs were prepared by a modified Watanabe method, and characterized by XRD and TEM. The biosensor was constructed by immobilizing the PtPd-MWCNTs catalysts in a Nafion film on a glassy carbon electrode. An inner Naﬁon film coating was used to eliminate common interferents such as uric acid, ascorbic acid and fructose. Finally, a highly porous surface with an orderly three-dimensional network enzyme layer (CS-GA-GOx) was fabricated by electrodeposition. The resulting biosensor exhibited a good response to glucose with a wide linear range (0.062-14.07 mM) and a low detection limit 0.031 mM. The biosensor also showed a short response time (within 5 s), and a high sensitivity (112 μA mM(-1)cm(-2)). The Michaelis-Menten constant (K(m)) was determined as 3.3 mM. In addition, the biosensor exhibited high reproducibility, good storage stability and satisfactory anti-interference ability. The applicability of the biosensor to actual serum sample analysis was also evaluated.
authors.affiliation: Nanoelectrochemistry Laboratory, Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 106, Taiwan, ROC.
authors.forename: Kuan-Jung
authors.initials: KJ
authors.lastname: Chen
authors.forename: Chia-Feng
authors.initials: CF
authors.lastname: Lee
authors.forename: John
authors.initials: J
authors.lastname: Rick
authors.forename: Shih-Han
authors.initials: SH
authors.lastname: Wang
authors.forename: Chung-Chiun
authors.initials: CC
authors.lastname: Liu
authors.forename: Bing-Joe
authors.initials: BJ
authors.lastname: Hwang
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D001786:Blood Glucose / Q000032:analysis*; D002384:Catalysis; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010165:Palladium / Q000737:chemistry; D010984:Platinum / Q000737:chemistry; D015203:Reproducibility of Results
chemical_list: D001786:Blood Glucose; D037742:Nanotubes, Carbon; D010984:Platinum; D010165:Palladium