title: A dynamic study on reversal of multidrug resistance by ginsenoside Rh₂ in adriamycin-resistant human breast cancer MCF-7 cells.
abstract: The quartz crystal microbalance (QCM) dynamic measurements indicate that ginsenoside Rh(2) (G-Rh(2)) could inhibit the proliferation of adriamycin-resistant human breast cancer MCF-7 cells (MCF-7/ADR) in a concentration-dependent way. The combined treatment of G-Rh(2) with adriamycin (ADR) at non-effect dosage resulted in the higher inhibition efficiencies and the increased cell-death velocity, suggesting excellent ability of G-Rh(2) for reversal of multidrug resistance in MCF-7/ADR cells. The cytotoxic effect of the ADR-G-Rh(2) combination was evaluated with the modified Bürgi formula (Jin equation) based on the QCM responses. It presented apparent synergism, indicating the potential ability of G-Rh(2) in tumor therapy. Fluorescent microscopic inspection and methyl thiazolyl tetrazolium (MTT) assay were also carried out and exhibited the comparable results to QCM analysis. The present work may lay an experimental foundation for the application of ginsenosides in cancer therapy, especial in multidrug resistance research.
authors.affiliation: Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education of China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
authors.forename: Bei
authors.initials: B
authors.lastname: Zhou
authors.forename: Xiuli
authors.initials: X
authors.lastname: Xiao
authors.forename: Lili
authors.initials: L
authors.lastname: Xu
authors.forename: Lian
authors.initials: L
authors.lastname: Zhu
authors.forename: Liang
authors.initials: L
authors.lastname: Tan
authors.forename: Hao
authors.initials: H
authors.lastname: Tang
authors.forename: Youyu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Qingji
authors.initials: Q
authors.lastname: Xie
authors.forename: Shouzhuo
authors.initials: S
authors.lastname: Yao
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D001943:Breast Neoplasms; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004317:Doxorubicin / Q000494:pharmacology*; D019008:Drug Resistance, Neoplasm / Q000187:drug effects*; D004357:Drug Synergism; D005260:Female; D036145:Ginsenosides / Q000494:pharmacology*; D006801:Humans; D008856:Microscopy, Fluorescence; D058525:Quartz Crystal Microbalance Techniques
chemical_list: D000970:Antineoplastic Agents; D036145:Ginsenosides; C055305:ginsenoside Rh2; D004317:Doxorubicin