title: A nested sequence-specific primer-polymerase chain reaction for the detection of HLA-B*15:02.
abstract: In this study, we reported a new technique in detecting HLA-B*15:02 by using a nested sequence-specific primer-polymerase chain reaction (SSP-PCR) that can be used on genomic DNA and whole blood for carbamazepine hypersensitivity prediction. We tested a total of 200 blind samples with known human leukocyte antigen (HLA)-B allelic types (44 positive for HLA-B*15:02 and 156 negative for HLA-B*15:02) with this new nested SSP-PCR technique and compared its efficacy to that of commercial sequence-specific oligonucleotide probe-polymerase chain reaction (SSOP-PCR). Using starting materials from DNA and whole blood, we were able to detect HLA-B*15:02 in 44 of our samples correctly. The test is very sensitive and is highly reproducible.
authors.affiliation: Medical Microbiology, Interdisciplinary Program, Graduate School, Chulalongkorn University, Bangkok, Thailand.
authors.forename: S
authors.initials: S
authors.lastname: Virakul
authors.forename: P
authors.initials: P
authors.lastname: Kupatawintu
authors.forename: J
authors.initials: J
authors.lastname: Nakkuntod
authors.forename: O
authors.initials: O
authors.lastname: Kangwanshiratada
authors.forename: T
authors.initials: T
authors.lastname: Vilaivan
authors.forename: N
authors.initials: N
authors.lastname: Hirankarn
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D015235:HLA-B Antigens / Q000737:chemistry / Q000235:genetics; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D015235:HLA-B Antigens