title: Protective effect of dextrans on glucose oxidase denaturation and inactivation.
abstract: In the present study, the stabilizing effect of dextrans as additives on the denaturation and inactivation of glucose oxidase (GOD) was investigated. Three different molecular weighted dextrans (M(w) 17.5, 75, 188 kD) were used with different concentrations. Dramatically increased enzyme activities were measured after one hour of incubation of enzyme with additives between 25-40°C in water bath. Highest activity value was measured with 75 kDa molecular weighted dextran (in concentration 30% w/v) at pH 5. Dextran as an additive supplied a long shelf-life to the enzyme at 4°C. In the presence of the 75 kDa dextran, the enzyme was more stable and its activity was increased 2.7-fold at 30°C. In addition, dextran protected GOD against inactivation by a n-heptane/aqueous buffer-stirred system.
authors.affiliation: Yildiz Technical University, Faculty of Arts and Sciences, Department of Chemistry, Istanbul, Turkey. maltikatoglu@yahoo.com
authors.forename: Melda
authors.initials: M
authors.lastname: Altikatoglu
authors.forename: Yeliz
authors.initials: Y
authors.lastname: Basaran-Elalmis
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology; D002021:Buffers; D003911:Dextrans / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D005949:Glucose Oxidase / Q000737:chemistry* / Q000378:metabolism*; D008970:Molecular Weight; D010952:Plasma Substitutes / Q000737:chemistry / Q000494:pharmacology; D011489:Protein Denaturation / Q000187:drug effects*; D013696:Temperature; D013997:Time Factors
chemical_list: D002021:Buffers; D003911:Dextrans; D010952:Plasma Substitutes; D005949:Glucose Oxidase