title: Stimulation of angiogenesis in rat ischemic limb by intramuscular implantation of mononuclear fraction cells from autologous bone marrow.
abstract: We studied angiogenic effects of two variants of indirect revascularization (transplantation of mononuclear fraction cells from autologous bone marrow alone or in combination with laser tunneling) after modeled ischemia of the lower limbs in rats. Doppler sonography and immunofluorescence studies showed that intramuscular implantation of isolated fraction of autologous bone marrow mononuclear cells in combination with laser tunneling of the muscles is most effective and can be recommended as a method of angiogenesis stimulation in non-reconstructable distal vascular pathology of the extremities.
authors.affiliation: E. N. Meshalkin Research Institute of Circulatory Pathology, Federal Agency for High-Technological Medical Care, Novosibirsk, Russia. pathol@soramn.ru
authors.forename: P M
authors.initials: PM
authors.lastname: Larionov
authors.forename: A M
authors.initials: AM
authors.lastname: Chernyavskii
authors.forename: R B
authors.initials: RB
authors.lastname: Novruzov
authors.forename: E L
authors.initials: EL
authors.lastname: Lushnikova
authors.forename: D V
authors.initials: DV
authors.lastname: Subbotin
authors.forename: D S
authors.initials: DS
authors.lastname: Sergeevichev
authors.forename: I V
authors.initials: IV
authors.lastname: Kuznetsova
authors.forename: A M
authors.initials: AM
authors.lastname: Karas'kov
authors.forename: L M
authors.initials: LM
authors.lastname: Nepomnyashchikh
mesh_terms: D043925:Angiogenesis Inducing Agents; D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology; D016026:Bone Marrow Transplantation / Q000379:methods*; D005263:Femoral Artery / Q000473:pathology / Q000601:surgery; D007511:Ischemia / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D053685:Laser Therapy / Q000379:methods; D007963:Leukocytes, Mononuclear / Q000637:transplantation; D035002:Lower Extremity / Q000098:blood supply* / Q000473:pathology / Q000503:physiopathology; D018919:Neovascularization, Physiologic; D058729:Peripheral Arterial Disease / Q000628:therapy*; D051381:Rats; D017208:Rats, Wistar; D014182:Transplantation, Autologous
chemical_list: D043925:Angiogenesis Inducing Agents