title: The targeted delivery of anticancer drugs to brain glioma by PEGylated oxidized multi-walled carbon nanotubes modified with angiopep-2.
abstract: In this study, a dual-targeting drug delivery system based on PEGylated oxidized multi-walled carbon nanotubes (O-MWNTs) modified with angiopep-2 (O-MWNTs-PEG-ANG) was successfully developed for treatment of brain glioma. O-MWNTs can not only distribute in brains but also accumulate in tumors, and have ultrahigh surface area with remarkably high loading anticancer drug of doxorubicin (DOX), which was selected as drug carrier. Angiopep-2 can specifically combine to the low-density lipoprotein receptor-related protein (LRP) receptor overexpressed on the blood-brain barrier (BBB) and glioma cells, which was selected as targeting ligand. The cooperative dual-targeting to brain glioma by O-MWNTs-PEG-ANG was evaluated by intracellular tracking in vitro and fluorescence imaging in vivo, which demonstrated that the combination of O-MWNTs-PEG and angiopep-2 constituted an ideal dual-targeting drug delivery system. The anti-glioma effect of DOX-loaded O-MWNTs-PEG-ANG (DOX-O-MWNTs-PEG-ANG) was assessed by C6 cytotoxicity and median survival time of glioma bearing mice, which showed a better anti-glioma effect than DOX. The biological safety of O-MWNTs-PEG-ANG was evaluated by BCEC and C6 cytotoxicity, hematology analysis and CD68 immunohistochemical analysis, which proved O-MWNTs-PEG-ANG was good biocompatibility and low toxicity. The biological safety of DOX-O-MWNTs-PEG-ANG was evaluated by histopathological analysis, which suggested a lower cardiac toxicity than DOX. In conclusion, O-MWNTs-PEG-ANG is a promising dual-targeting carrier to deliver DOX for the treatment of brain tumor.
authors.affiliation: School of Pharmacy & Key Laboratory of Smart Drug Delivery, Ministry of Education & PLA, Fudan University, Shanghai 200032, China.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Ren
authors.forename: Shun
authors.initials: S
authors.lastname: Shen
authors.forename: Dangge
authors.initials: D
authors.lastname: Wang
authors.forename: Zhangjie
authors.initials: Z
authors.lastname: Xi
authors.forename: Liangran
authors.initials: L
authors.lastname: Guo
authors.forename: Zhiqing
authors.initials: Z
authors.lastname: Pang
authors.forename: Yong
authors.initials: Y
authors.lastname: Qian
authors.forename: Xiyang
authors.initials: X
authors.lastname: Sun
authors.forename: Xinguo
authors.initials: X
authors.lastname: Jiang
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage / Q000737:chemistry; D001932:Brain Neoplasms / Q000188:drug therapy* / Q000473:pathology; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000008:administration & dosage* / Q000737:chemistry; D005910:Glioma / Q000188:drug therapy* / Q000473:pathology; D006801:Humans; D053769:Nanocapsules / Q000008:administration & dosage / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010084:Oxidation-Reduction; D010455:Peptides / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D016896:Treatment Outcome
chemical_list: C531860:Angiopep-2; D000903:Antibiotics, Antineoplastic; D053769:Nanocapsules; D037742:Nanotubes, Carbon; D010455:Peptides; D011092:Polyethylene Glycols; D004317:Doxorubicin