title: Regular and moderate exercise before experimental sepsis reduces the risk of lung and distal organ injury.
abstract: Physical activity modulates inflammation and immune response in both normal and pathologic conditions. We investigated whether regular and moderate exercise before the induction of experimental sepsis reduces the risk of lung and distal organ injury and survival. One hundred twenty-four BALB/c mice were randomly assigned to two groups: sedentary (S) and trained (T). Animals in T group ran on a motorized treadmill, at moderate intensity, 5% grade, 30 min/day, 3 times a week for 8 wk. Cardiac adaptation to exercise was evaluated using echocardiography. Systolic volume and left ventricular mass were increased in T compared with S group. Both T and S groups were further randomized either to sepsis induced by cecal ligation and puncture surgery (CLP) or sham operation (control). After 24 h, lung mechanics and histology, the degree of cell apoptosis in lung, heart, kidney, liver, and small intestine villi, and interleukin (IL)-6, KC (IL-8 murine functional homolog), IL-1β, IL-10, and number of cells in bronchoalveolar lavage (BALF) and peritoneal lavage (PLF) fluids as well as plasma were measured. In CLP, T compared with S groups showed: 1) improvement in survival; 2) reduced lung static elastance, alveolar collapse, collagen and elastic fiber content, number of neutrophils in BALF, PLF, and plasma, as well as lung and distal organ cell apoptosis; and 3) increased IL-10 in BALF and plasma, with reduced IL-6, KC, and IL-1β in PLF. In conclusion, regular and moderate exercise before the induction of sepsis reduced the risk of lung and distal organ damage, thus increasing survival.
authors.affiliation: Laboratory of Pulmonary Investigation, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Brazil.
authors.forename: Carla C
authors.initials: CC
authors.lastname: de Araújo
authors.forename: Johnatas D
authors.initials: JD
authors.lastname: Silva
authors.forename: Cynthia S
authors.initials: CS
authors.lastname: Samary
authors.forename: Isabela H
authors.initials: IH
authors.lastname: Guimarães
authors.forename: Patrícia S
authors.initials: PS
authors.lastname: Marques
authors.forename: Gisele P
authors.initials: GP
authors.lastname: Oliveira
authors.forename: Luana G R R
authors.initials: LG
authors.lastname: do Carmo
authors.forename: Regina C
authors.initials: RC
authors.lastname: Goldenberg
authors.forename: Ilka
authors.initials: I
authors.lastname: Bakker-Abreu
authors.forename: Bruno L
authors.initials: BL
authors.lastname: Diaz
authors.forename: Nazareth N
authors.initials: NN
authors.lastname: Rocha
authors.forename: Vera L
authors.initials: VL
authors.lastname: Capelozzi
authors.forename: Paolo
authors.initials: P
authors.lastname: Pelosi
authors.forename: Patricia R M
authors.initials: PR
authors.lastname: Rocco
mesh_terms: D055371:Acute Lung Injury / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D000332:Aerobiosis; D000818:Animals; D017209:Apoptosis / Q000502:physiology; D001202:Ascitic Fluid / Q000502:physiology; D001992:Bronchoalveolar Lavage Fluid; D002432:Cecum / Q000502:physiology; D004452:Echocardiography; D016753:Interleukin-10 / Q000097:blood; D053208:Kaplan-Meier Estimate; D008026:Ligation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D046529:Microscopy, Electron, Transmission; D010805:Physical Conditioning, Animal / Q000502:physiology*; D015656:Respiratory Mechanics / Q000502:physiology; D018805:Sepsis / Q000150:complications* / Q000473:pathology; D013534:Survival
chemical_list: D016753:Interleukin-10