title: IMP-29, a novel IMP-type metallo-β-lactamase in Pseudomonas aeruginosa.
abstract: Analysis of two clonally related multiresistant Pseudomonas aeruginosa isolates led to the identification of a novel IMP-type metallo-β-lactamase. IMP-29 was significantly different from the other IMP variants (the closest variant being IMP-5 with 93% amino acid identity). The bla(IMP-29) gene cassette was carried by a class 1 integron in strain 10.298, while in strain 10.266 it was located in a rearranged DNA region on a 30-kb conjugative plasmid. Biochemical analysis confirmed that IMP-29 efficiently hydrolyzed carbapenems.
authors.affiliation: Centre National de la Résistance aux Antibiotiques, Laboratoire associé Pseudomonas aeruginosa, Hôpital Jean Minjoz, Besançon, France.
authors.forename: Katy
authors.initials: K
authors.lastname: Jeannot
authors.forename: Laurent
authors.initials: L
authors.lastname: Poirel
authors.forename: Marjorie
authors.initials: M
authors.lastname: Robert-Nicoud
authors.forename: Pascal
authors.initials: P
authors.lastname: Cholley
authors.forename: Patrice
authors.initials: P
authors.lastname: Nordmann
authors.forename: Patrick
authors.initials: P
authors.lastname: Plésiat
mesh_terms: D000368:Aged; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D015780:Carbapenems / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D017809:Fatal Outcome; D006801:Humans; D006868:Hydrolysis; D032023:Integrons / Q000235:genetics; D008297:Male; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D011552:Pseudomonas Infections / Q000188:drug therapy / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000235:genetics*; D012772:Shock, Septic / Q000188:drug therapy / Q000209:etiology; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D015780:Carbapenems; D004269:DNA, Bacterial; C573059:beta lactamase IMP-29, Pseudomonas aeruginosa; D001618:beta-Lactamases