title: Livin regulates prostate cancer cell invasion by impacting the NF-κB signaling pathway and the expression of FN and CXCR4.
abstract: Prostate cancer (PCa) has the second highest mortality rate of all tumor-related diseases for males in Western countries, and the incidence of PCa in China is increasing. Previous studies have proven that inhibitor of apoptosis proteins (IAPs) can regulate tumor cell invasion and metastasis. Livin is the most recently identified IAP. Our previous study showed that Livin might play an important role in the initiation of human PCa and that Livin-α might promote cell proliferation by regulating the G1-S cell cycle transition. However, whether Livin, as an IAP, can regulate the invasive ability of PCa cells remains unknown. In this study, we found that the expression of Livin was higher in metastatic PCa tissues than in nonmetastatic tissues and that the expression of Livin was downregulated/upregulated by small interfering RNA/vector, which could inhibit/promote PC-3/LNCaP cell invasion. This action was related to the impact of Livin on nuclear factor-κB (NF-κB) and its downstream signaling pathway, including FN and CXCR4. Together, our findings suggested that Livin might regulate tumor cell invasion in PCa directly, and that Livin might be an ideal candidate for preventing tumor cell invasion.
authors.affiliation: Department of Urology, First Affiliated Hospital of Dalian Medical University, Liaoning, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.forename: Deyong
authors.initials: D
authors.lastname: Yang
authors.forename: Shujing
authors.initials: S
authors.lastname: Wang
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Che
authors.forename: Jianbo
authors.initials: J
authors.lastname: Wang
authors.forename: Xiancheng
authors.initials: X
authors.lastname: Li
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Zhang
authors.forename: Xiaochi
authors.initials: X
authors.lastname: Chen
authors.forename: Xishuang
authors.initials: X
authors.lastname: Song
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D017209:Apoptosis; D015153:Blotting, Western; D002448:Cell Adhesion; D002453:Cell Cycle; D002465:Cell Movement; D049109:Cell Proliferation; D005353:Fibronectins / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D051034:Inhibitor of Apoptosis Proteins / Q000378:metabolism*; D008297:Male; D016328:NF-kappa B / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology*; D019718:Receptors, CXCR4 / Q000378:metabolism*; D015398:Signal Transduction; D014407:Tumor Cells, Cultured
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C419866:BIRC7 protein, human; D005353:Fibronectins; D051034:Inhibitor of Apoptosis Proteins; D016328:NF-kappa B; D009363:Neoplasm Proteins; D019718:Receptors, CXCR4