title: The cough receptor TRPV1 agonists 15(S)-HETE and LTB4 in the cough response to hypertonicity.
abstract: Asthmatic patients are hypersensitive to the cough-provoking effect of hypertonic aerosols. 15- hydroxyeicosatetraenoic acid (15(S)-HETE) and leukotriene (LT) B4 are asthma-related mediators which can be released upon hypertonic stimuli, and both are potent agonists of the transient receptor potential vanilloid subfamily member 1 (TRPV1), a major cough receptor. Therefore, they are potential mediators for hypertonicity-provoked cough. Twenty-six asthmatic and ten healthy subjects underwent a hypertonic saline cough provocation test. Exhaled breath condensate was collected before and after the test, and the concentrations of 15(S)-HETE and LTB4 were analysed. Neither the baseline concentrations of these mediators nor the saline test-induced changes in them were associated with cough responsiveness to hypertonicity. High baseline 15(S)-HETE was associated with aspirin hypersensitivity and high LTB4 with male sex and large variability in ambulatory peak flow measurements. The TRPV1 agonists 15(S)-HETE and LTB4 seem not to be involved in the cough response to hypertonicity in asthmatic patients.
authors.affiliation: Unit of Medicine and Clinical Research, Pulmonary Division, Kuopio University Hospital, Kuopio, Finland. heikki.koskela@kuh.fi
authors.forename: Heikki
authors.initials: H
authors.lastname: Koskela
authors.forename: Minna
authors.initials: M
authors.lastname: Purokivi
authors.forename: Riina
authors.initials: R
authors.lastname: Nieminen
authors.forename: Eeva
authors.initials: E
authors.lastname: Moilanen
mesh_terms: D000328:Adult; D001249:Asthma / Q000503:physiopathology*; D001985:Bronchial Provocation Tests; D003371:Cough / Q000139:chemically induced / Q000378:metabolism*; D005260:Female; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000032:analysis / Q000378:metabolism*; D007975:Leukotriene B4 / Q000032:analysis / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D012462:Saline Solution, Hypertonic; D050916:TRPV Cation Channels / Q000819:agonists*
chemical_list: D006893:Hydroxyeicosatetraenoic Acids; D012462:Saline Solution, Hypertonic; D050916:TRPV Cation Channels; C494482:TRPV1 protein, human; D007975:Leukotriene B4