title: Single collateral reconstructions reveal distinct phases of corticospinal remodeling after spinal cord injury.
abstract: BACKGROUND
  Injuries to the spinal cord often result in severe functional deficits that, in case of incomplete injuries, can be partially compensated by axonal remodeling. The corticospinal tract (CST), for example, responds to a thoracic transection with the formation of an intraspinal detour circuit. The key step for the formation of the detour circuit is the sprouting of new CST collaterals in the cervical spinal cord that contact local interneurons. How individual collaterals are formed and refined over time is incompletely understood.
  
  
  RESULTS
  We traced the hindlimb corticospinal tract at different timepoints after lesion to show that cervical collateral formation is initiated in the first 10 days. These collaterals can then persist for at least 24 weeks. Interestingly, both major and minor CST components contribute to the formation of persistent CST collaterals. We then developed an approach to label single CST collaterals based on viral gene transfer of the Cre recombinase to a small number of cortical projection neurons in Thy1-STP-YFP or Thy1-Brainbow mice. Reconstruction and analysis of single collaterals for up to 12 weeks after lesion revealed that CST remodeling evolves in 3 phases. Collateral growth is initiated in the first 10 days after lesion. Between 10 days and 3-4 weeks after lesion elongated and highly branched collaterals form in the gray matter, the complexity of which depends on the CST component they originate from. Finally, between 3-4 weeks and 12 weeks after lesion the size of CST collaterals remains largely unchanged, while the pattern of their contacts onto interneurons matures.
  
  
  CONCLUSIONS
  This study provides a comprehensive anatomical analysis of CST reorganization after injury and reveals that CST remodeling occurs in distinct phases. Our results and techniques should facilitate future efforts to unravel the mechanisms that govern CST remodeling and to promote functional recovery after spinal cord injury.
authors.affiliation: Research Unit Therapy Development, Institute of Clinical Neuroimmunology, Ludwig-Maximilians-Universität München, Munich, Germany.
authors.forename: Claudia
authors.initials: C
authors.lastname: Lang
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Guo
authors.forename: Martin
authors.initials: M
authors.lastname: Kerschensteiner
authors.forename: Florence M
authors.initials: FM
authors.lastname: Bareyre
mesh_terms: D000818:Animals; D001369:Axons / Q000378:metabolism / Q000473:pathology / Q000502:physiology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D058948:Cell Tracking / Q000379:methods; D005260:Female; D007091:Image Processing, Computer-Assisted / Q000379:methods; D007395:Interneurons / Q000378:metabolism / Q000473:pathology / Q000502:physiology; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008954:Models, Biological; D009416:Nerve Regeneration / Q000502:physiology*; D011712:Pyramidal Tracts / Q000473:pathology / Q000503:physiopathology; D020127:Recovery of Function / Q000502:physiology; D013119:Spinal Cord Injuries / Q000175:diagnosis / Q000503:physiopathology* / Q000534:rehabilitation*; D013997:Time Factors
chemical_list: D001426:Bacterial Proteins; D008164:Luminescent Proteins; C054562:yellow fluorescent protein, Bacteria