title: Overexpression of Arabidopsis dehydration- responsive element-binding protein 2C confers tolerance to oxidative stress.
abstract: Dehydration-responsive element-binding proteins (DREBs)regulate plant responses to environmental stresses. In the current study, transcription of DREB2C, a class 2 Arabidopsis DREB, was induced by a superoxide anion propagator, methyl viologen (MV). The oxidative stress tolerance of DREB2C-overexpressing transgenic plants was significantly greater than that of wild-type plants, as measured by ion leakage and chlorophyll fluorescence under light conditions. The transcriptional activity of several ascorbate peroxidase (APX) genes as well as APX protein activity was induced in DREB2C overexpressors. Additionally, the level of H2O2 in the overexpressors was lower than in wt plants under similar oxidative stress conditions. An electrophoretic mobility shift assay and transient activator reporter assay showed that APX2 expression was regulated by heat shock factor A3 (HsfA3) and that HsfA3 is regulated at the transcriptional level by DREB2C. These results suggest that DREB2C plays an important role in promoting oxidative stress tolerance in Arabidopsis.
authors.affiliation: Systems and Synthetic Agrobiotech Center and PMBBRC, Gyeongsang National University, Jinju 660-701, Korea.
authors.forename: Jung Eun
authors.initials: JE
authors.lastname: Hwang
authors.forename: Chan Ju
authors.initials: CJ
authors.lastname: Lim
authors.forename: Huan
authors.initials: H
authors.lastname: Chen
authors.forename: Jihyun
authors.initials: J
authors.lastname: Je
authors.forename: Chieun
authors.initials: C
authors.lastname: Song
authors.forename: Chae Oh
authors.initials: CO
authors.lastname: Lim
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D060387:Ascorbate Peroxidases / Q000235:genetics / Q000378:metabolism; D002470:Cell Survival / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D000076249:Heat Shock Transcription Factors; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006540:Herbicides / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000378:metabolism; D018384:Oxidative Stress; D010269:Paraquat / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D011485:Protein Binding; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D015533:Transcriptional Activation / Q000187:drug effects / Q000235:genetics; D019076:Transgenes / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; C573537:DREB2C protein, Arabidopsis; C000620136:HSFA3 protein, Arabidopsis; D000076249:Heat Shock Transcription Factors; D006360:Heat-Shock Proteins; D006540:Herbicides; D010940:Plant Proteins; D014157:Transcription Factors; D006861:Hydrogen Peroxide; D060387:Ascorbate Peroxidases; D010269:Paraquat