title: Bioactivity of freeze-dried platelet-rich plasma in an adsorbed form on a biodegradable polymer material.
abstract: Owing to the necessity for the immediate preparation from patients' blood, autologous platelet-rich plasma (PRP) limits its clinical applicability. To address this concern and respond to emergency care and other unpredictable uses, we have developed a freeze-dried PRP in an adsorbed form on a biodegradable polymer material (Polyglactin 910). On the polymer filaments of PRP mesh, which was prepared by coating the polymer mesh with human fresh PRP and subsequent freeze-drying, platelets were incorporated, and related growth factors were preserved at high levels. This new PRP mesh preparation significantly and reproducibly stimulated the proliferation of human periodontal ligament cells in vitro and neovascularization in a chorioallantoic membrane assay. A full-thickness skin defect model in a diabetic mouse demonstrated the PRP mesh, although prepared from human blood, substantially facilitated angiogenesis, granulation tissue formation, and re-epithelialization without inducing severe inflammation in vivo. These data demonstrate that our new PRP mesh preparation functions as a bioactive material to facilitate tissue repair/regeneration. Therefore, we suggest that this bioactive material, composed of allogeneic PRP, could be clinically used as a promising alternative in emergency care or at times when autologous PRP is not prepared immediately before application.
authors.affiliation: Division of Oral Bioengineering, Department of Tissue Regeneration and Reconstitution, Institute of Medicine and Dentistry, Niigata University, Niigata, Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Nakajima
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Kawase
authors.forename: Mito
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Okuda
authors.forename: Larry F
authors.initials: LF
authors.lastname: Wolff
authors.forename: Hiromasa
authors.initials: H
authors.lastname: Yoshie
mesh_terms: D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D001792:Blood Platelets / Q000502:physiology*; D049109:Cell Proliferation / Q000187:drug effects; D002642:Chick Embryo; D049033:Chorioallantoic Membrane / Q000098:blood supply* / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism / Q000473:pathology; D005612:Freeze Drying; D006097:Granulation Tissue / Q000187:drug effects; D006801:Humans; D051379:Mice; D018919:Neovascularization, Physiologic; D010513:Periodontal Ligament / Q000187:drug effects / Q000473:pathology; D010976:Platelet Count; D053657:Platelet-Rich Plasma / Q000737:chemistry*; D011098:Polyglactin 910 / Q000737:chemistry / Q000494:pharmacology; D012867:Skin / Q000098:blood supply* / Q000293:injuries; D013526:Surgical Mesh; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D001672:Biocompatible Materials; D011098:Polyglactin 910