title: Gene expression profile differences in embryos derived from prepubertal and adult Japanese Black cattle during in vitro development.
abstract: The present study was carried out to compare the gene expression profiles of in vitro-generated embryos derived from adult and prepubertal Japanese Black cattle oocytes using GeneChip Bovine Genome Array (containing 24072 probe sets representing over 23000 transcripts). Microarray experiments were performed on populations of 8- to 16-cell stage embryos and blastocysts derived from adult (24-35 months old) versus prepubertal (9-10 months old) Japanese Black cattle oocytes matured and fertilised in vitro. In total, 591 (2.4%) and 490 (2.0%) genes were differentially expressed in prepubertal and adult bovine in 8- to 16-cell and blastocyst stage embryos, respectively. Out of these, 218 and 248 genes were upregulated, while 373 and 242 were downregulated in prepubertal and adult 8- to 16-cell and blastocysts stage embryos, respectively. Gene ontology classification regarding biological process, molecular functions and cellular component revealed diversity in transcript abundances between prepubertal and adult groups in both the distinct developmental stages. Quantitative reverse transcription-PCR validated the expression differences of some selected transcripts as identified by microarray analysis. To our knowledge, this is the first report indicating the significant number of genes differentially expression (>2-fold, P<0.01) in preimplantition embryos between adult and prepubertal Japanese Black cattle during in vitro development.
authors.affiliation: Laboratory of Animal Reproduction, United Graduate School of Agricultural Science, Kagoshima University, Korimoto 1-21-24, Kagoshima 890-0065, Japan.
authors.lastname: Dorji
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Ohkubo
authors.forename: Kazuchika
authors.initials: K
authors.lastname: Miyoshi
authors.forename: Mitsutoshi
authors.initials: M
authors.lastname: Yoshida
mesh_terms: D000367:Age Factors; D000818:Animals; D002417:Cattle / Q000196:embryology* / Q000235:genetics*; D002478:Cells, Cultured; D046149:Embryo Culture Techniques; D004622:Embryo, Mammalian / Q000378:metabolism*; D047108:Embryonic Development / Q000235:genetics* / Q000502:physiology; D005260:Female; D005307:Fertilization in Vitro; D020869:Gene Expression Profiling; D046228:Microarray Analysis; D011247:Pregnancy; D012741:Sexual Maturation / Q000235:genetics / Q000502:physiology*; D013045:Species Specificity