title: Inhibition of androgen-responsive LNCaP prostate cancer cell tumor xenograft             growth by dietary phenethyl isothiocyanate correlates with decreased angiogenesis             and inhibition of cell attachment.
abstract: Phenethyl isothiocyanate (PEITC) is a candidate anticancer compound found             in certain cruciferous vegetables. In our tumor cell xenograft model, dietary             administration of PEITC (100-150 mg/kg body weight/d) inhibited androgen-responsive             LNCaP human prostate cancer cell tumor growth. We found that dietary treatment             with PEITC significantly inhibited tumor platelet/endothelial cell adhesion molecule             (PECAM-1/CD31) expression, a marker of angiogenesis. By contrast, we did not find             the inhibitory effects of PEITC on tumor growth to be associated with alteration             of specific markers for apoptosis, cell proliferation or androgen receptor-mediated             pathways. Consistent with in vivo results, PEITC exerted little effects on cell             proliferation, cell cycle and androgen-dependent pathways. Interestingly, PEITC             significantly attenuated LNCaP cell plating efficiency that correlated with inhibition             of integrin family proteins integrin β1, α2 and α6 mRNA expression. Thus, PEITC             may be a dietary factor that inhibits androgen-responsive prostate tumor growth             indirectly by selectively targeting factors involved in the tumor microenvironment.
authors.affiliation: Laboratory of Cellular Regulation and Carcinogenesis, National                     Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: Tamaro S
authors.initials: TS
authors.lastname: Hudson
authors.forename: Susan N
authors.initials: SN
authors.lastname: Perkins
authors.forename: Stephen D
authors.initials: SD
authors.lastname: Hursting
authors.forename: Heather A
authors.initials: HA
authors.lastname: Young
authors.forename: Young S
authors.initials: YS
authors.lastname: Kim
authors.forename: Tien-Chung
authors.initials: TC
authors.lastname: Wang
authors.forename: Thomas T Y
authors.initials: TT
authors.lastname: Wang
mesh_terms: D020533:Angiogenesis Inhibitors / Q000008:administration & dosage; D000818:Animals; D016588:Anticarcinogenic Agents / Q000008:administration & dosage; D048708:Cell Growth Processes / Q000187:drug effects; D021982:Cell-Matrix Junctions / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D017879:Isothiocyanates / Q000008:administration & dosage*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D011471:Prostatic Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D023041:Xenograft Model Antitumor Assays
chemical_list: D020533:Angiogenesis Inhibitors; D016588:Anticarcinogenic Agents; D017879:Isothiocyanates; C058305:phenethyl isothiocyanate