title: Active surveillance of methicillin-resistant Staphylococcus aureus with the BD GeneOhm MRSA™ assay in a respiratory ward in Nagasaki, Japan.
abstract: The utility of active surveillance cultures (ASCs) in respiratory wards, that do not  have an associated intensive care unit (ICU), and the usefulness of the BD GeneOhm MRSA™ system  for rapid detection of methicillin-resistant Staphylococcus aureus (MRSA) have not been previously  evaluated in Japan. ASCs using conventional culture methods and the BD GeneOhm MRSA™ assay were  conducted in adult inpatients between May 11, 2009 and November 10, 2009 in a respiratory ward,  without an associated ICU, in Nagasaki University Hospital. The infection and colonization rates  of MRSA acquired in this respiratory ward were both investigated. A total of 159 patients were  investigated. Of these, 12 (7.5%) were found positive for MRSA by the BD GeneOhm MRSA™ assay and  9 (5.7%) were found positive by a conventional culture test upon admission. All cases were  MRSA-colonized cases and cross-transmission was not found to occur during hospitalization. The BD  GeneOhm MRSA™ assay had a sensitivity of 100% and a specificity of 98%. ASCs in our respiratory  ward revealed that MRSA was brought in from other sites in some cases, and that current infection  control measures in Nagasaki University Hospital are effective. The BD GeneOhm MRSA™ assay was  proven to be a useful and rapid detection tool for MRSA.
authors.affiliation: Department of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Izumikawa
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Katsunori
authors.initials: K
authors.lastname: Yanagihara
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Kiya
authors.forename: Junichi
authors.initials: J
authors.lastname: Matsuda
authors.forename: Yoshitomo
authors.initials: Y
authors.lastname: Morinaga
authors.forename: Shintaro
authors.initials: S
authors.lastname: Kurihara
authors.forename: Shigeki
authors.initials: S
authors.lastname: Nakamura
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Imamura
authors.forename: Taiga
authors.initials: T
authors.lastname: Miyazaki
authors.forename: Tomoya
authors.initials: T
authors.lastname: Nishino
authors.forename: Misuzu
authors.initials: M
authors.lastname: Tsukamoto
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kakeya
authors.forename: Akira
authors.initials: A
authors.lastname: Yasuoka
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Tashiro
authors.forename: Shimeru
authors.initials: S
authors.lastname: Kamihira
authors.forename: Shigeru
authors.initials: S
authors.lastname: Kohno
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001431:Bacteriological Techniques; D005260:Female; D006761:Hospitals; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008297:Male; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000382:microbiology
chemical_list: D011933:Reagent Kits, Diagnostic