title: Mouse ES cells maintained in different pluripotency-promoting conditions differ in their neural differentiation propensity.
abstract: Prior to differentiation, embryonic stem (ES) cells in culture are maintained in a so-called "undifferentiated" state, allowing derivation of multiple downstream cell lineages when induced in a directed manner, which in turn grants these cells their "pluripotent" state. The current work is based on a simple observation that the initial culture condition for maintaining mouse ES cells in an "undifferentiated" state does impact on the differentiation propensity of these cells, in this case to a neuronal fate. We point out the importance in judging the "pluripotency" of a given stem cell culture, as this clearly demonstrated that the "undifferentiated" state of these cells is not necessarily a "pluripotent" state, even for a widely used mouse ES cell line. We partly attribute this difference in the initial value of ES cells to the naïve-to-primed status of pluripotency, which in turn may affect early events of the differentiation in vitro.
authors.affiliation: Department of Regenerative Medicine, National Cerebral and Cardiovascular Center, 5-7-1 Fujishiro-dai, Suita, Osaka, Japan, 565-8565.
authors.forename: Haruka
authors.initials: H
authors.lastname: Hirose
authors.forename: Hidemasa
authors.initials: H
authors.lastname: Kato
authors.forename: Akie
authors.initials: A
authors.lastname: Kikuchi-Taura
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Soma
authors.forename: Akihiko
authors.initials: A
authors.lastname: Taguchi
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D002460:Cell Line / Q000166:cytology*; D019070:Cell Lineage; D016895:Culture Media, Serum-Free; D053595:Embryonic Stem Cells / Q000166:cytology*; D051379:Mice; D009474:Neurons / Q000166:cytology*; D039904:Pluripotent Stem Cells / Q000166:cytology*
chemical_list: D016895:Culture Media, Serum-Free