title: Differential response of Drosophila cell lines to extracellular adenosine.
abstract: Adenosine (Ado) is a crucial metabolite that affects a wide range of physiological processes. Key proteins regulating Ado signaling, transport and metabolism are conserved among vertebrates and invertebrates. It is well known that Ado influences proliferation of several vertebrate and invertebrate cells. Here we show that Ado negatively influences viability, changes morphology and mitochondrial polarity of the Drosophila imaginal disc cell line (Cl.8+) via a mechanism exclusively dependent on cellular Ado uptake. High transport of Ado is followed by phosphorylation and ATP production as a part of Ado salvation, which at higher concentrations may interfere with cellular homeostasis. In contrast, hematopoietic cell line Mbn2, which grows well in high Ado concentration, preferentially uses adenosine deaminase as a part of the purine catabolic pathway. Our results show that different types of Drosophila cell lines use different pathways for Ado conversion and suggest that such differences may be an important part of complex mechanisms maintaining energy homeostasis in the body.
authors.affiliation: Univ. of South Bohemia, Ceske Budejovice, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Fleischmannova
authors.forename: Lucie
authors.initials: L
authors.lastname: Kucerova
authors.forename: Katerina
authors.initials: K
authors.lastname: Sandova
authors.forename: Veronika
authors.initials: V
authors.lastname: Steinbauerova
authors.forename: Vaclav
authors.initials: V
authors.lastname: Broz
authors.forename: Petr
authors.initials: P
authors.lastname: Simek
authors.forename: Michal
authors.initials: M
authors.lastname: Zurovec
mesh_terms: D000241:Adenosine / Q000378:metabolism* / Q000633:toxicity; D000248:Adenosine Kinase / Q000037:antagonists & inhibitors / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D004330:Drosophila / Q000166:cytology / Q000378:metabolism*; D004734:Energy Metabolism; D008297:Male; D014529:Uridine / Q000378:metabolism
chemical_list: D000255:Adenosine Triphosphate; D000248:Adenosine Kinase; D000241:Adenosine; D014529:Uridine