title: MicroRNA-449a acts as a tumor suppressor in human bladder cancer through the regulation of pocket proteins.
abstract: Frequent downregulation of microRNA-449a (miR-449a) was detected in 14 human bladder cancer tissues. The restoration of miR-449a inhibited cell growth and induced G1-phase arrest in T24 and 5637 human bladder cancer cells. CDK6 and CDC25a were downregulated after miR-449a treatment, resulting in the functional accumulation of the pocket proteins Rb and p130. The growth of T24 tumor xenografts was suppressed by exogenous miR-449a, and the nuclear proliferation antigen Ki-67 was downregulated in miR-449a-treated tumors. These results suggest a tumor-suppressive role for miR-449a in human bladder cancer.
authors.affiliation: Department of Urology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.forename: Yi-wei
authors.initials: YW
authors.lastname: Lin
authors.forename: Ye-qing
authors.initials: YQ
authors.lastname: Mao
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
authors.forename: Yun-fu
authors.initials: YF
authors.lastname: Liu
authors.forename: Xiang-yi
authors.initials: XY
authors.lastname: Zheng
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Xie
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D001483:Base Sequence; D048708:Cell Growth Processes / Q000235:genetics; D045744:Cell Line, Tumor; D050719:Crk-Associated Substrate Protein / Q000235:genetics* / Q000378:metabolism; D051361:Cyclin-Dependent Kinase 6 / Q000037:antagonists & inhibitors; D015536:Down-Regulation; D005260:Female; D059585:G1 Phase Cell Cycle Checkpoints / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000008:administration & dosage / Q000096:biosynthesis / Q000235:genetics*; D008875:Middle Aged; D024101:Mitochondrial Proteins; D008969:Molecular Sequence Data; D010766:Phosphorylation; D016160:Retinoblastoma Protein / Q000235:genetics* / Q000378:metabolism; D014183:Transplantation, Heterologous; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D020687:cdc25 Phosphatases / Q000037:antagonists & inhibitors
chemical_list: C493841:BCAR1 protein, human; D050719:Crk-Associated Substrate Protein; C544530:MIRN449 microRNA, human; D035683:MicroRNAs; D024101:Mitochondrial Proteins; D016160:Retinoblastoma Protein; C558496:SPATA18 protein, human; C495911:CDK6 protein, human; D051361:Cyclin-Dependent Kinase 6; C517004:CDC25A protein, human; D020687:cdc25 Phosphatases