title: Molecular characterization of signalling pathways in cancer stem cells.
abstract: To avoid artefacts introduced by culturing cells for extended periods of time, it is crucial to use low-passage patient-derived tumour cells. The ability to enrich, isolate and assay sub-populations of cells that behave as cancer stem cells (CSCs) from these primary cell lines is essential before performing characterizations such as gene-expression profiling. We have isolated cells from glioblastomas which show characteristics of CSCs. Although glioblastomas contain only a relatively small amount of putative CSCs, these cells express many genes which seem to be worthy targets for future therapies.
authors.affiliation: MPI for Molecular Genetics, Berlin, Germany. mail@christian-regenbrecht.de
authors.forename: C
authors.initials: C
authors.lastname: Regenbrecht
authors.forename: Y
authors.initials: Y
authors.lastname: Welte
authors.forename: A
authors.initials: A
authors.lastname: Jodicke
authors.forename: R
authors.initials: R
authors.lastname: Hugel
authors.forename: P
authors.initials: P
authors.lastname: Walden
authors.forename: M
authors.initials: M
authors.lastname: Jung
authors.forename: H
authors.initials: H
authors.lastname: Lehrach
authors.forename: J
authors.initials: J
authors.lastname: Adjaye