title: Irradiated stem cells and ageing of the haematopoietic system.
abstract: In the work presented here, changes in haematopoiesis of mice (B6129SF2/J) were studied 1 year after their whole-body exposure to a dose of 7 Gy (72% of mice survived). The irradiated mice were compared with non-irradiated younger (4 months of age) and older (16 months of age) mice. There was a significant increase in the relative abundance of primitive stem cells with long-term capability of the haematopoiesis recovery lin(-)/Sca-1(+)/CD117(+)/CD34(-) in the bone marrow of mice aged 16 months (irradiated and non-irradiated) compared with those aged 4 months. In terms of the ability to respond to further whole-body irradiation at a dose of 1 Gy, the presence of γH2A.X foci was studied in lin(-) bone marrow cells. There was a considerable number of persisting foci in lin(-) stem cells isolated from the bone marrow of the older irradiated mice. In the blood count from the peripheral blood of the older mice (both non-irradiated and irradiated at 7 Gy), there was a significant increase in granulocytes. In the group exposed to 7 Gy, the numbers of thrombocytes significantly increased, and on the contrary, the numbers of erythrocytes, the amount of haemoglobin, and haematocrit significantly decreased.
authors.affiliation: Department of Radiobiology, Faculty of Health Sciences, University of Defence in Brno, Hradec Králové, Czech Republic. vavrova@pmfhk.cz
authors.forename: Jiřina
authors.initials: J
authors.lastname: Vávrová
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Sinkorová
authors.forename: Martina
authors.initials: M
authors.lastname: Rezáčová
authors.forename: Aleš
authors.initials: A
authors.lastname: Tichý
authors.forename: Stanislav
authors.initials: S
authors.lastname: Filip
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Mokrý
authors.forename: Emilie
authors.initials: E
authors.lastname: Lukášová
mesh_terms: D000367:Age Factors; D000818:Animals; D001792:Blood Platelets / Q000378:metabolism / Q000528:radiation effects; D001853:Bone Marrow / Q000528:radiation effects; D002460:Cell Line; D004912:Erythrocytes / Q000378:metabolism / Q000528:radiation effects; D005260:Female; D006098:Granulocytes / Q000378:metabolism / Q000528:radiation effects; D006410:Hematopoiesis / Q000528:radiation effects*; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*; D006454:Hemoglobins / Q000378:metabolism / Q000528:radiation effects; D006657:Histones / Q000378:metabolism; D008297:Male; D051379:Mice; D014916:Whole-Body Irradiation / Q000379:methods
chemical_list: D006454:Hemoglobins; D006657:Histones; C475961:gamma-H2AX protein, mouse