title: Fast, selective, and sensitive analysis of low-abundance peptides in human plasma by electromembrane extraction.
abstract: A totally new concept based on electrokinetic migration was evaluated for the extraction of three biologically active peptides from human plasma. Angiotensin 2, leu-enkephalin, and endomorphin 1 migrated from a diluted human plasma sample (2 mL, positive electrode), through a supported liquid membrane (SLM) of 1-octanol, di-isobutylketon, and di-(2-ethylhexyl) phosphate (DEHP) (55:35:10, w/w/w), and into an acidified acceptor solution (25 μL 50 mM HCl, negative electrode) by the application of an electrical potential (20 V) across the SLM. After only five min of extraction, the acceptor solution was injected and analyzed directly by liquid chromatography. The three peptides were quantified by tandem mass spectrometry, with acceptable linearity ranging from 100.0 to 1000.0 pg mL(-1) (r(2) in the range 0.9736-0.9988), and repeatability (RSD) ranging between 15% and 24% (n=5), using plasma spiked with the three peptides in 100 pg mL(-1) concentration. The estimated detection limits (S/N ratio of 3:1) for angiotensin 2, leu-enkephalin, and endomorphin 1, were 60, 24, and 24 pg mL(-1), respectively. With this novel approach based on electromembrane extraction (EME) coupled to LC-MS/MS, endogenous concentrations of the peptides were detected in non-spiked human plasma samples, with a total analysis time less than 50 min. These experimental findings were highly interesting, and showed the opportunities for EME with regard to future peptide extractions.
authors.affiliation: Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, Oslo, Norway. marte.balchen@farmasi.uio.no
authors.forename: Marte
authors.initials: M
authors.lastname: Balchen
authors.forename: Hanne
authors.initials: H
authors.lastname: Lund
authors.forename: Léon
authors.initials: L
authors.lastname: Reubsaet
authors.forename: Stig
authors.initials: S
authors.lastname: Pedersen-Bjergaard
mesh_terms: D020003:1-Octanol / Q000737:chemistry; D000804:Angiotensin II / Q000097:blood / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D004051:Diethylhexyl Phthalate / Q000737:chemistry; D004566:Electrodes; D004743:Enkephalin, Leucine / Q000097:blood / Q000302:isolation & purification; D006801:Humans; D007659:Ketones / Q000737:chemistry; D008567:Membranes, Artificial; D009842:Oligopeptides / Q000097:blood / Q000302:isolation & purification; D010455:Peptides / Q000097:blood* / Q000302:isolation & purification; D053719:Tandem Mass Spectrometry
chemical_list: D007659:Ketones; D008567:Membranes, Artificial; D009842:Oligopeptides; D010455:Peptides; C105293:endomorphin 1; D000804:Angiotensin II; D004743:Enkephalin, Leucine; D004051:Diethylhexyl Phthalate; D020003:1-Octanol; C054815:diisobutyl ketone