title: Optimization of Lipase Production from Aspergillus terreus by Response Surface Methodology and Its Potential for Synthesis of Partial Glycerides Under Solvent Free Conditions.
abstract: Aspergillus terreus produces lipase 7.01 IU/ml in 96 h after optimization by one variable at a time method. Using the significant factors i.e. corn oil (A), sodium nitrate (B), casein (C), agitation rate (D) and incubation period (E) RSM was carried out resulting in 19.65 IU/ml from the combination +1(A), -1(B), -1(C), +1(D) and 0(E). The interactions between sodium nitrate, casein and agitation with corn oil were most significant. Scale up of production from 250 ml shake flask to 30 l bioreactor resulted in increased productivity of 0.52 IU/ml/h as against 0.2 IU/ml/h obtained in shake flasks. This lipase could carryout solvent free synthesis of partial glycerides of oleic acid with 96% efficiency in 12 h.
authors.forename: Rekha
authors.initials: R
authors.lastname: Kaushik
authors.forename: Ruchi Gulati
authors.initials: RG
authors.lastname: Marwah
authors.forename: Pritesh
authors.initials: P
authors.lastname: Gupta
authors.forename: Saurabh
authors.initials: S
authors.lastname: Saran
authors.forename: Luciano
authors.initials: L
authors.lastname: Saso
authors.forename: V S
authors.initials: VS
authors.lastname: Parmar
authors.forename: R K
authors.initials: RK
authors.lastname: Saxena