title: Synthesis and characterization of a potential prebiotic trisaccharide from cheese whey permeate and sucrose by Leuconostoc mesenteroides dextransucrase.
abstract: The production of new bioactive oligosaccharides is currently garnering much attention for their potential use as functional ingredients. This work addresses the enzymatic synthesis and NMR structural characterization of 2-α-D-glucopyranosyl-lactose derived from sucrose:lactose and sucrose:cheese whey permeate mixtures by using a Leuconostoc mesenteroides B-512F dextransucrase. The effect of synthesis conditions, including concentration of substrates, molar ratio of donor/acceptor, enzyme concentration, reaction time, and temperature, on the formation of transfer products is evaluated. Results indicated that cheese whey permeate is a suitable material for the synthesis of 2-α-D-glucopyranosyl-lactose, giving rise to yields around 50% (in weight respect to the initial amount of lactose) under the optimum reaction conditions. According to its structure, this trisaccharide is an excellent candidate for a new prebiotic ingredient, due to the reported high resistance of α-(1→2) linkages to the digestive enzymes in humans and animals, as well as to its potential selective stimulation of beneficial bacteria in the large intestine mainly attributed to the two linked glucose units located at the reducing end that reflects the disaccharide kojibiose (2-α-D-glucopyranosyl-D-glucose). These findings could contribute to broadening the use of important agricultural raw materials, such as sucrose or cheese whey permeates, as renewable substrates for enzymatic synthesis of oligosaccharides of nutritional interest.
authors.affiliation: Dpto. Bioactividad y Análisis de Alimentos, Instituto de Investigación en Ciencias de la Alimentación, CIAL (CSIC-UAM), CEI (UAM+CSIC), c/Nicolás Cabrera 9, 28049 Madrid, Spain.
authors.forename: Marina
authors.initials: M
authors.lastname: Díez-Municio
authors.forename: Antonia
authors.initials: A
authors.lastname: Montilla
authors.forename: M Luisa
authors.initials: ML
authors.lastname: Jimeno
authors.forename: Nieves
authors.initials: N
authors.lastname: Corzo
authors.forename: Agustín
authors.initials: A
authors.lastname: Olano
authors.forename: F Javier
authors.initials: FJ
authors.lastname: Moreno
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D002611:Cheese / Q000032:analysis* / Q000382:microbiology*; D005525:Food-Processing Industry / Q000191:economics; D005964:Glucosyltransferases / Q000378:metabolism*; D007220:Industrial Waste / Q000032:analysis / Q000191:economics; D007934:Leuconostoc / Q000201:enzymology*; D056692:Prebiotics / Q000032:analysis* / Q000191:economics; D013030:Spain; D013395:Sucrose / Q000378:metabolism*; D014312:Trisaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: C572879:2-alpha-D-glucopyranosyl-lactose; D001426:Bacterial Proteins; D007220:Industrial Waste; D056692:Prebiotics; D014312:Trisaccharides; D013395:Sucrose; D005964:Glucosyltransferases; C020025:dextransucrase