title: Transcription-independent role of Bach1 in mitosis through a nuclear exporter Crm1-dependent mechanism.
abstract: The transcriptional repressor Bach1 mediates various stress responses. Despite its role in transcription, Bach1 is predominantly exported to the cytoplasm in a Crm1-dependent manner, but the functional role of its cytoplasmic retention is still unclear. We found that Bach1 was also excluded from mitotic chromatin by a C-terminal cytoplasmic localization sequence dependent and leptomycin B sensitive process. Bach1 depletion resulted in disordered mitotic chromosome alignment, which was rescued by Bach1 mutants lacking the BTB or DNA binding domains, suggesting its transcription-independent mechanism. We thus revealed a novel role of Bach1 in the regulation of mitotic chromosome dynamics.
authors.affiliation: Department of Biochemistry, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Takuma
authors.initials: T
authors.lastname: Shiraki
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Igarashi
mesh_terms: D021581:Active Transport, Cell Nucleus; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D050976:Basic-Leucine Zipper Transcription Factors / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D023902:Chromosome Pairing / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D017931:DNA Primers / Q000235:genetics; D051856:Fanconi Anemia Complementation Group Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology; D055785:Gene Knockdown Techniques; D057809:HEK293 Cells; D006801:Humans; D028884:Karyopherins / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D008938:Mitosis / Q000235:genetics* / Q000502:physiology*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D011743:Pyrimidines / Q000494:pharmacology; D034741:RNA, Small Interfering / Q000235:genetics; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics* / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D013871:Thiones / Q000494:pharmacology; D014158:Transcription, Genetic; D000097606:Exportin 1 Protein
chemical_list: D050976:Basic-Leucine Zipper Transcription Factors; D017931:DNA Primers; D051856:Fanconi Anemia Complementation Group Proteins; D005231:Fatty Acids, Unsaturated; D028884:Karyopherins; D011743:Pyrimidines; D034741:RNA, Small Interfering; D018160:Receptors, Cytoplasmic and Nuclear; D013871:Thiones; D000097606:Exportin 1 Protein; C102108:BACH1 protein, human; C486316:Bach1 protein, mouse; C400223:monastrol; C038753:leptomycin B