title: Chimeric classical swine fever (CSF)-Japanese encephalitis (JE) viral replicon as a non-transmissible vaccine candidate against CSF and JE infections.
abstract: A trans-complemented chimeric CSF-JE virus replicon was constructed using an infectious cDNA clone of the CSF virus (CSFV) Alfort/187 strain. The CSFV E2 gene was deleted, and a fragment containing the region encoding a truncated envelope protein (tE, amino acid 292-402, domain III) of JE virus (JEV) was inserted into the resultant plasmid, pA187delE2, to generate the recombinant cDNA clone pA187delE2/JEV-tE. Porcine kidney 15 (PK15) cells that constitutively express the CSFV E2p7 proteins were then transfected with in vitro-transcribed RNA from pA187delE2/JEV-tE. As a result, the chimeric CSF-JE virus replicon particle (VRP), rv187delE2/JEV-tE, was rescued. In a mouse model, immunization with the chimeric CSF-JE VRP induced strong production of JEV-specific antibody and conferred protection against a lethal JEV challenge. Pigs immunized with CSF-JE VRP displayed strong anti-CSFV and anti-JEV antibody responses and protection against CSFV and JEV challenge infections. Our evidence suggests that E2-complemented CSF-JE VRP not only has potential as a live-attenuated non-transmissible vaccine candidate against CSF and JE but also serves as a potential DIVA (Differentiating Infected from Vaccinated Animals) vaccine for CSF in pigs. Together, our data suggest that the non-transmissible chimeric VRP expressing foreign antigenic proteins may represent a promising strategy for bivalent DIVA vaccine design.
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Yang
authors.forename: Rui
authors.initials: R
authors.lastname: Wu
authors.forename: Robert W
authors.initials: RW
authors.lastname: Li
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.forename: Zhongliang
authors.initials: Z
authors.lastname: Xiong
authors.forename: Haizhong
authors.initials: H
authors.lastname: Zhao
authors.forename: Deyin
authors.initials: D
authors.lastname: Guo
authors.forename: Zishu
authors.initials: Z
authors.lastname: Pan
mesh_terms: D000818:Animals; D002460:Cell Line; D002678:Chimera / Q000235:genetics / Q000276:immunology* / Q000502:physiology; D006691:Classical Swine Fever / Q000276:immunology / Q000517:prevention & control / Q000821:virology*; D006692:Classical Swine Fever Virus / Q000235:genetics / Q000276:immunology* / Q000502:physiology; D004664:Encephalitis Virus, Japanese / Q000235:genetics / Q000276:immunology* / Q000502:physiology; D004672:Encephalitis, Japanese / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D012093:Replicon; D013552:Swine; D014613:Vaccines, Attenuated / Q000235:genetics / Q000276:immunology; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology; D014779:Virus Replication
chemical_list: D014613:Vaccines, Attenuated; D014764:Viral Proteins; D014765:Viral Vaccines