title: Development of an immunoassay for terbutryn: study of the influence of the immunization protocol.
abstract: Heterologous immunization has proven to be useful to enhance the selectivity and specificity of catalytic antibodies. However, in the field of immunoassays, few studies have been done to establish how the immunization protocol influences the antibody characteristics. In the present study, we have developed an enzyme-linked immunosorbent assay (ELISA) for the detection of the pesticide terbutryn following a homologous and a heterologous immunization strategy. No significant differences have been observed between the immunization procedures regarding immunoassay sensitivity and selectivity. Thus, immunoassays with a limit of detection below the 25 ng/l established by current European regulations have been obtained with both immunization protocols. Initial studies have been performed to assess the applicability of these ELISAs to the analysis of real water matrixes.
authors.affiliation: Applied Molecular Receptors Group (AMRg), Institute for Advanced Chemistry of Catalonia (IQAC) of Spanish Council for Scientific Research (CSIC), Jordi Girona 18-26, 08034 Barcelona, Spain.
authors.forename: Nuria
authors.initials: N
authors.lastname: Sanvicens
authors.forename: Begoña
authors.initials: B
authors.lastname: Varela
authors.forename: Berta
authors.initials: B
authors.lastname: Ballesteros
authors.forename: M-Pilar
authors.initials: MP
authors.lastname: Marco
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology; D000917:Antibody Formation; D001667:Binding, Competitive; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005620:Freund's Adjuvant / Q000276:immunology; D006241:Haptens / Q000737:chemistry* / Q000276:immunology; D006433:Hemocyanins / Q000737:chemistry / Q000276:immunology; D007114:Immunization / Q000379:methods*; D007700:Kinetics; D010573:Pesticide Residues / Q000097:blood* / Q000276:immunology; D011485:Protein Binding; D011817:Rabbits; D012680:Sensitivity and Specificity; D014227:Triazines / Q000097:blood* / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D006241:Haptens; D010573:Pesticide Residues; D014227:Triazines; D005620:Freund's Adjuvant; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin; C010347:terbutryne