title: Genome sequence of Wickerhamomyces anomalus DSM 6766 reveals genetic basis of biotechnologically important antimicrobial activities.
abstract: The ascomycetous yeast Wickerhamomyces anomalus (formerly Pichia anomala and Hansenula anomala) exhibits antimicrobial activities and flavoring features that are responsible for its frequent association with food, beverage and feed products. However, limited information on the genetic background of this yeast and its multiple capabilities are currently available. Here, we present the draft genome sequence of the neotype strain W. anomalus DSM 6766. On the basis of pyrosequencing, a de novo assembly of this strain resulted in a draft genome sequence with a total size of 25.47 Mbp. An automatic annotation using RAPYD generated 11 512 protein-coding sequences. This annotation provided the basis to analyse metabolic capabilities, phylogenetic relationships, as well as biotechnologically important features and yielded novel candidate genes of W. anomalus DSM 6766 coding for proteins participating in antimicrobial activities.
authors.affiliation: Center for Biotechnology, Bielefeld University, Bielefeld, Germany.
authors.forename: Jessica
authors.initials: J
authors.lastname: Schneider
authors.forename: Oliver
authors.initials: O
authors.lastname: Rupp
authors.forename: Eva
authors.initials: E
authors.lastname: Trost
authors.forename: Sebastian
authors.initials: S
authors.lastname: Jaenicke
authors.forename: Volkmar
authors.initials: V
authors.lastname: Passoth
authors.forename: Alexander
authors.initials: A
authors.lastname: Goesmann
authors.forename: Andreas
authors.initials: A
authors.lastname: Tauch
authors.forename: Karina
authors.initials: K
authors.lastname: Brinkrolf
mesh_terms: D000890:Anti-Infective Agents / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods*; D002874:Chromosome Mapping; D005516:Food Microbiology; D005656:Fungal Proteins / Q000378:metabolism; D016681:Genome, Fungal / Q000235:genetics*; D055646:Killer Factors, Yeast / Q000378:metabolism*; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D010802:Phylogeny; D010843:Pichia / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA / Q000379:methods*; D055549:Volatile Organic Compounds / Q000378:metabolism*
chemical_list: D000890:Anti-Infective Agents; D005656:Fungal Proteins; D055646:Killer Factors, Yeast; D055549:Volatile Organic Compounds; C087906:killer toxin, Pichia