title: Evaluation of Coccidioides antigen detection in dogs with coccidioidomycosis.
abstract: Antigen detection has been reported to be a promising method for rapid diagnosis of coccidioidomycosis in humans. Coccidioides antigen detection has not been previously reported in dogs with coccidioidomycosis and was evaluated in 60 cases diagnosed based on detection of anti-Coccidioides antibodies at titers of 1:16 or more in serum. Controls included dogs with presumed histoplasmosis or blastomycosis, other fungal infections, or nonfungal diseases and healthy dogs. Urine and serum specimens were tested using an enzyme immunoassay for Coccidioides galactomannan antigen. Antibody testing was performed at commercial veterinary reference laboratories. Antigen was detected in urine or serum of 12 of 60 (20.0%), urine only in 2 of 57 (3.5%), and serum only in 11 of 58 (19.0%) dogs with coccidioidomycosis. Antigen was detected in the urine of 3 of 43 (7.0%) and serum of 1 of 37 (2.7%) dogs with histoplasmosis or blastomycosis but not in 13 dogs with other fungal infections (serum, 9; urine, 13), 41 dogs with nonfungal diseases (urine, 41; serum, 18), or healthy dogs (serum, 21; urine, 21). Detection of antigen was an insensitive method for diagnosis of coccidioidomycosis in dogs in which the diagnosis was based primarily upon detection of antibodies at titers of 1:16 or higher, and the highest sensitivity was in serum.
authors.affiliation: MiraVista Diagnostics, Indianapolis, Indiana, USA. ekirsch@miravistalabs.com
authors.forename: Emily J
authors.initials: EJ
authors.lastname: Kirsch
authors.forename: Russell T
authors.initials: RT
authors.lastname: Greene
authors.forename: Annalisa
authors.initials: A
authors.lastname: Prahl
authors.forename: Stanley I
authors.initials: SI
authors.lastname: Rubin
authors.forename: Jane E
authors.initials: JE
authors.lastname: Sykes
authors.forename: Michelle M
authors.initials: MM
authors.lastname: Durkin
authors.forename: Lawrence J
authors.initials: LJ
authors.lastname: Wheat
mesh_terms: D000818:Animals; D000908:Antibodies, Fungal / Q000097:blood; D000946:Antigens, Fungal / Q000032:analysis*; D003045:Coccidioides / Q000302:isolation & purification*; D003047:Coccidioidomycosis / Q000175:diagnosis / Q000662:veterinary*; D004283:Dog Diseases / Q000175:diagnosis* / Q000382:microbiology; D004285:Dogs; D005690:Galactose / Q000031:analogs & derivatives; D007124:Immunoenzyme Techniques / Q000379:methods; D008351:Mannans / Q000032:analysis*; D012680:Sensitivity and Specificity; D044967:Serum / Q000382:microbiology; D014556:Urine / Q000382:microbiology
chemical_list: D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D008351:Mannans; C012990:galactomannan; D005690:Galactose