title: Development of a miniature analytical system in a lab-on-valve for determination of trace copper by bead injection spectroscopy.
abstract: A miniature analytical system based on a lab-on-valve platform is developed for trace metal analysis by bead injection spectroscopy. A multipurpose flow cell integrated into a lab-on-valve is furnished with two pieces of fiber optics to communicate with light source and charge coupled device (CCD) spectrometer, respectively, in order to monitor real-time absorbance of the samples. Micro-beads loaded with chromogenic reagent are packed into the multipurpose flow cell to form a renewable microcolumn for solid phase extraction by bead injection. When the sample solution flows through the microcolumn, the target analyte will be captured on the surface of beads and detected directly by the CCD spectrometer without elution. The beads are automatically discarded from the multipurpose flow cell after each analytical cycle. This analytical system was employed to determine trace copper by loading of a chromogenic reagent 2-carboxy-2'-hydroxy-5'-sulfoformazylbenzene (zincon) on the beads of an anion exchanger (Sephadex QAE A-25). With a sample volume of 2.5mL, a detection limit of 3μgL(-1) and a linear range of 10-100μgL(-1) were obtained for copper, along with a RSD value of 2.5% (at the 50μgL(-1) level). The accuracy and practical applicability of the proposed system were validated by analysing certified reference materials, i.e., GBW10010, GBW09101, GBW08608, and further demonstrated by spiking recovery of copper in a water sample.
authors.affiliation: Department of Chemistry, Northeastern University, Box 332, Shenyang 110819, China.
authors.forename: Yong-Liang
authors.initials: YL
authors.lastname: Yu
authors.forename: Ying
authors.initials: Y
authors.lastname: Jiang
authors.forename: Rong-Huan
authors.initials: RH
authors.lastname: He
mesh_terms: D003300:Copper / Q000032:analysis*; D003911:Dextrans; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods; D005562:Formazans / Q000737:chemistry; D005618:Fresh Water / Q000737:chemistry; D006197:Hair / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D008863:Microspheres; D055100:Optical Fibers; D012275:Oryza / Q000737:chemistry; D012015:Reference Standards; D052616:Solid Phase Extraction / Q000379:methods; D013057:Spectrum Analysis / Q000295:instrumentation / Q000379:methods; D055672:Static Electricity; D014131:Trace Elements / Q000032:analysis*
chemical_list: D003911:Dextrans; D005562:Formazans; D014131:Trace Elements; C009809:2-carboxy-2'-hydroxy-5'-sulfoformazylbenzene; D003300:Copper; C025614:sephadex