title: Mitochondrial-nuclear DNA interactions contribute to the regulation of nuclear transcript levels as part of the inter-organelle communication system.
abstract: Nuclear and mitochondrial organelles must maintain a communication system. Loci on the mitochondrial genome were recently reported to interact with nuclear loci. To determine whether this is part of a DNA based communication system we used genome conformation capture to map the global network of DNA-DNA interactions between the mitochondrial and nuclear genomes (Mito-nDNA) in Saccharomyces cerevisiae cells grown under three different metabolic conditions. The interactions that form between mitochondrial and nuclear loci are dependent on the metabolic state of the yeast. Moreover, the frequency of specific mitochondrial-nuclear interactions (i.e. COX1-MSY1 and Q0182-RSM7) showed significant reductions in the absence of mitochondrial encoded reverse transcriptase machinery. Furthermore, these reductions correlated with increases in the transcript levels of the nuclear loci (MSY1 and RSM7). We propose that these interactions represent an inter-organelle DNA mediated communication system and that reverse transcription of mitochondrial RNA plays a role in this process.
authors.affiliation: Institute of Natural Sciences, Massey University, Albany, New Zealand.
authors.forename: Chris D M
authors.initials: CD
authors.lastname: Rodley
authors.forename: Ralph S
authors.initials: RS
authors.lastname: Grand
authors.forename: Lutz R
authors.initials: LR
authors.lastname: Gehlen
authors.forename: Gary
authors.initials: G
authors.lastname: Greyling
authors.forename: M Beatrix
authors.initials: MB
authors.lastname: Jones
authors.forename: Justin M
authors.initials: JM
authors.lastname: O'Sullivan
mesh_terms: D001692:Biological Transport / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D002467:Cell Nucleus / Q000187:drug effects / Q000235:genetics*; D015825:Chromosomes, Fungal / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D051545:Cyclooxygenase 1 / Q000235:genetics / Q000378:metabolism; D004272:DNA, Mitochondrial / Q000187:drug effects / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004843:Epistasis, Genetic / Q000187:drug effects / Q000502:physiology; D005690:Galactose / Q000494:pharmacology; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D056426:Genetic Loci / Q000502:physiology; D005947:Glucose / Q000494:pharmacology; D015388:Organelles / Q000187:drug effects / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D012331:RNA, Fungal / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D004272:DNA, Mitochondrial; D004268:DNA-Binding Proteins; D012331:RNA, Fungal; D012333:RNA, Messenger; D051545:Cyclooxygenase 1; D005947:Glucose; D005690:Galactose