title: [Preparation and identification of monoclonal antibody against alpha-momorcharins].
abstract: Three BALB/c mice were immunized four times with alpha-momorcharins (alpha-MMC). Using polyethylene glycol (PEG) method, the immunized splenocytes were fused with SP2/0 cells. One strain of hybridoma cells was obtained which secrete antibodies against alpha-MMC. To get ascites, the hybridoma cells were injected into the abdominal cavity of mice. The antibodies were purified from ascites. Indirect enzyme linked immunosorbent assay (ELISA) and Western blot assay were applied to determine the specifity of the monoclonal antibody (McAb). The results showed that the McAb was specific to alpha-MMC without detectable cross-activity with MAP30. The McAb provided detecting method for further research of the structure and function of alpha-MMC.
authors.affiliation: Institute for Nanobiomedical Technology and Membrane Biology, Sichuan University/College of Life Science, Sichuan University, Chengdu 610064, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Meng
authors.forename: Xingchuan
authors.initials: X
authors.lastname: Yao
authors.forename: Shurui
authors.initials: S
authors.lastname: Wang
authors.forename: Min
authors.initials: M
authors.lastname: Lin
authors.forename: Hongning
authors.initials: H
authors.lastname: Wang
authors.forename: Yanfa
authors.initials: Y
authors.lastname: Meng
authors.forename: Xiaojan
authors.initials: X
authors.lastname: Zhao
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006825:Hybridomas / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D054788:Ribosome Inactivating Proteins / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; C039582:MMC protein, Momordica charantia; D054788:Ribosome Inactivating Proteins