title: High-sodium intake aggravates myocardial injuries induced by aldosterone via oxidative stress in Sprague-Dawley rats.
abstract: OBJECTIVE
  To evaluate the effects of aldosterone with or without high sodium intake on blood pressure, myocardial structure and left ventricular function in rats, and to investigate the mechanisms underlying the effects.
  
  
  METHODS
  Eight-week-old male Sprague-Dawley rats were randomly divided into 3 groups: (1) control (CON) group fed a normal sodium diet, (2) aldosterone (ALD) group receiving aldosterone infusion and a normal sodium diet, and (3) high sodium plus aldosterone (HS-ALD) group receiving 1% NaCl diet in conjunction with aldosterone infusion. Aldosterone was administered through continuously subcutaneous infusion with osmotic minipump at the rate of 0.75 μg/h for 8 weeks. The myocardium structure was observed using transthoracic echocardiography and transmission electron microscopy. The collagen deposition in left ventricle was evaluated with Masson's trichrome staining. The expression of IL-18, p22phox, and p47phox proteins was examined using Western blot analysis.
  
  
  RESULTS
  The systolic blood pressure in the ALD and HS-ALD groups was significantly higher than that in the CON group after 2-week treatment. But the blood pressure showed no significant difference between the HS-ALD and ALD groups. The left ventricular hypertrophy, myocardial collagen deposition and oxidative stress were predominantly found in the HS-ALD and ALD group. Furthermore, the breakdown of myocardial structure and oxidative stress were more apparent in the HS-ALD group as compared with those in the ALD group.
  
  
  CONCLUSIONS
  Long-term infusion of aldosterone results in hypertension and profibrotic cardiovascular responses in rats fed a normal sodium diet, which were mediated by oxidative stress. High-sodium intake could aggravate myocardial injuries induced by aldosterone.
authors.affiliation: Department of Endocrinology, The Second Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
authors.forename: Jing-yi
authors.initials: JY
authors.lastname: Li
authors.forename: Shao-ling
authors.initials: SL
authors.lastname: Zhang
authors.forename: Meng
authors.initials: M
authors.lastname: Ren
authors.forename: Yan-ling
authors.initials: YL
authors.lastname: Wen
authors.forename: Li
authors.initials: L
authors.lastname: Yan
authors.forename: Hua
authors.initials: H
authors.lastname: Cheng
mesh_terms: D000450:Aldosterone / Q000633:toxicity*; D000818:Animals; D004357:Drug Synergism; D006321:Heart / Q000187:drug effects*; D008297:Male; D009206:Myocardium / Q000378:metabolism / Q000473:pathology*; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012982:Sodium, Dietary / Q000008:administration & dosage / Q000633:toxicity*
chemical_list: D012982:Sodium, Dietary; D000450:Aldosterone