title: Autosomal short tandem repeat analysis of ancient DNA by coupled use of mini- and conventional STR kits.
abstract: Multiplex autosomal short tandem repeat (STR) genotyping enables researchers to obtain genetic information from ancient human samples. In this study, we tested newly developed AmpFℓSTR(®) MiniFiler™ kit for autosomal STR analysis of ancient DNA (aDNA), using human femurs (n = 8) collected from medieval Korean tombs. After extracting aDNA from the bones, autosomal STR analyses were repeated for each sample using the AmpFℓSTR(®) MiniFiler™ and Identifiler™ kits. Whereas only 21.87% of larger-sized loci profiles could be obtained with the Identifiler™ kit, 75% of the same loci profiles were determined by MiniFiler™ kit analysis. This very successful amplification of large-sized STR markers from highly degraded aDNA suggests that the MiniFiler™ kit could be a useful complement to conventional STR kit analysis of ancient samples.
authors.affiliation: Institute of Forensic Medicine, Seoul National University College of Medicine, 28, Yongon-Dong, Chongno-Gu, Seoul 110-799, South Korea.
authors.forename: Chang Seok
authors.initials: CS
authors.lastname: Oh
authors.forename: Sang Jun
authors.initials: SJ
authors.lastname: Lee
authors.forename: Jun Bum
authors.initials: JB
authors.lastname: Park
authors.forename: Soong Deok
authors.initials: SD
authors.lastname: Lee
authors.forename: Seung Bum
authors.initials: SB
authors.lastname: Seo
authors.forename: Hye Yeon
authors.initials: HY
authors.lastname: Kim
authors.forename: Jaehyup
authors.initials: J
authors.lastname: Kim
authors.forename: Yi-Suk
authors.initials: YS
authors.lastname: Kim
authors.forename: Dong Hoon
authors.initials: DH
authors.lastname: Shin
mesh_terms: D004247:DNA / Q000302:isolation & purification; D053836:DNA Degradation, Necrotic; D016172:DNA Fingerprinting / Q000295:instrumentation* / Q000379:methods; D005269:Femur / Q000737:chemistry; D060005:Genotyping Techniques / Q000295:instrumentation / Q000379:methods; D049669:History, 16th Century; D049671:History, 18th Century; D006801:Humans; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction
chemical_list: D004247:DNA