title: MicroRNA-125b induces cancer cell apoptosis through suppression of Bcl-2 expression.
abstract: MicroRNAs (miRNAs) are small, noncoding RNAs which can often act as an oncogene or a tumor suppressor. Several miRNAs are associated with the development of hepatocellular carcinoma (HCC). We demonstrated that miR-125b significantly suppresses HCC cell proliferation and promotes apoptosis by inhibiting the gene expression of the anti-apoptotic protein, Bcl-2. Bioinformatic analysis indicated that the 3'UTR of Bcl-2 has binding sites for miR-125b. Luciferase reporter assay confirmed the ability of miR-125b to dramatically suppress Bcl-2 transcription, suggesting that Bcl-2 is a target gene for miR-125b. We concluded that miR-125b acts as a tumor suppressor in hepatic tumor development by targeting Bcl-2 and inducing cancer cell apoptosis.
authors.affiliation: Department of Obstetrics and Gynecology, General Hospital of PLA, Beijing, China.
authors.forename: Aihua
authors.initials: A
authors.lastname: Zhao
authors.forename: Quan
authors.initials: Q
authors.lastname: Zeng
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Xie
authors.forename: Junnian
authors.initials: J
authors.lastname: Zhou
authors.forename: Wen
authors.initials: W
authors.lastname: Yue
authors.forename: Yali
authors.initials: Y
authors.lastname: Li
authors.forename: Xuetao
authors.initials: X
authors.lastname: Pei
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D017209:Apoptosis / Q000235:genetics*; D001483:Base Sequence; D045744:Cell Line, Tumor; D018450:Disease Progression; D015536:Down-Regulation / Q000235:genetics*; D057809:HEK293 Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; C531888:MIRN125 microRNA, human; D035683:MicroRNAs; D019253:Proto-Oncogene Proteins c-bcl-2