title: Naked PCV-2 cloned genomic DNA is infectious by mucosal (intratracheal or oro-nasal) inoculation.
abstract: Porcine circovirus type 2 (PCV-2) is involved in several diseases named porcine circovirus-associated diseases and is transmitted by oro-faecal route. In this study we inoculated porcine-circovirus free piglets by mucosal routes (intratracheal or oro-nasal routes) with a plasmid carrying two copies of PCV-2 genomic DNA and compared the results to the intramuscular route. We observed that this PCV-2 naked DNA serves as template for viral replication and infectious PCV-2 particles are detected in the whole body after parenteral (intramuscular) or mucosal (intratracheal or oro-nasal) delivery. These results suggest that PCV-2 genome could play a role in in vivo transmission.
authors.affiliation: Anses, Laboratory of Ploufragan-Plouzané, BP 53, 22440 Ploufragan, France. beatrice.grasland@anses.fr
authors.forename: Béatrice
authors.initials: B
authors.lastname: Grasland
authors.forename: Lionel
authors.initials: L
authors.lastname: Bigault
authors.forename: Maëna
authors.initials: M
authors.lastname: Le Corvec
authors.forename: Anne-Cécile
authors.initials: AC
authors.lastname: Hernandez-Nignol
authors.forename: Aurélie
authors.initials: A
authors.lastname: Oger
authors.forename: Daniel
authors.initials: D
authors.lastname: Dory
authors.forename: Nicolas
authors.initials: N
authors.lastname: Rose
authors.forename: Roland
authors.initials: R
authors.lastname: Cariolet
authors.forename: André
authors.initials: A
authors.lastname: Jestin
mesh_terms: D000818:Animals; D018173:Circoviridae Infections / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism*; D009297:Nasal Mucosa / Q000821:virology; D020545:Respiratory Mucosa / Q000821:virology*; D013552:Swine; D013553:Swine Diseases / Q000821:virology*; D014132:Trachea / Q000821:virology*; D014774:Virulence
chemical_list: D004279:DNA, Viral