title: Development of a hybrid dextrin hydrogel encapsulating dextrin nanogel as protein delivery system.
abstract: Dextrin, a glucose polymer with low molecular weight, was used to develop a fully resorbable hydrogel, without using chemical initiators. Dextrin was first oxidized (oDex) with sodium periodate and then cross-linked with adipic acid dihidrazide, a nontoxic cross-linking molecule. Furthermore, a new bidimensional composite hydrogel, made of oxidized dextrin incorporating dextrin nanogels (oDex-nanogel), was also developed. The oDex hydrogels showed good mechanical properties and biocompatibility, allowing the proliferation of mouse embryo fibroblasts 3T3 cultured on top of the gel. The gelation time may be controlled selecting the concentrations of the polymer and reticulating agent. Both the oDex and oDex-nanogel hydrogels are biodegradable and present a 3-D network with a continuous porous structure. The obtained hybrid hydrogel enables the release of the dextrin nanogel over an extended period of time, paralleling the mass loss curve due to the degradation of the material. The dextrin nanogel allowed the efficient incorporation of interleukin-10 and insulin in the oDex hydrogel, providing a sophisticated system of controlled release. The new hydrogels present promising properties as an injectable carrier of bioactive molecules. Both proteins and poorly water-soluble low-molecular-weight drugs are efficiently encapsulated in the nanogel, which performs as a controlled release system entrapped in the hydrogel matrix.
authors.affiliation: Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
authors.forename: Maria
authors.initials: M
authors.lastname: Molinos
authors.forename: Vera
authors.initials: V
authors.lastname: Carvalho
authors.forename: Dina M
authors.initials: DM
authors.lastname: Silva
authors.forename: Francisco M
authors.initials: FM
authors.lastname: Gama
mesh_terms: D016475:3T3 Cells; D000272:Adipates / Q000737:chemistry; D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D001711:Biotransformation; D002470:Cell Survival; D003432:Cross-Linking Reagents / Q000737:chemistry; D003692:Delayed-Action Preparations / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D003912:Dextrins / Q000737:chemistry*; D016650:Fluorescein-5-isothiocyanate; D020100:Hydrogels / Q000737:chemistry*; D007328:Insulin / Q000008:administration & dosage / Q000737:chemistry; D016753:Interleukin-10 / Q000008:administration & dosage / Q000737:chemistry; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008855:Microscopy, Electron, Scanning; D000080385:Nanogels; D010504:Periodic Acid / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry / Q000378:metabolism; D016062:Porosity; D011506:Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D000272:Adipates; D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D003692:Delayed-Action Preparations; D003912:Dextrins; D020100:Hydrogels; D007328:Insulin; D000080385:Nanogels; D011108:Polymers; D011506:Proteins; C413692:polyethylene glycol polyethyleneimine nanogel; D010504:Periodic Acid; D016753:Interleukin-10; D011092:Polyethylene Glycols; D011094:Polyethyleneimine; C009288:metaperiodate; D016650:Fluorescein-5-isothiocyanate; C010011:adipic dihydrazide