title: A rationally designed pyrrolysyl-tRNA synthetase mutant with a broad substrate spectrum.
abstract: Together with tRNA(CUA)(Pyl), a rationally designed pyrrolysyl-tRNA synthetase mutant N346A/C348A has been successfully used for the genetic incorporation of a variety of phenylalanine derivatives with large para substituents into superfolder green fluorescent protein at an amber mutation site in Escherichia coli. This discovery greatly expands the genetically encoded noncanonical amino acid inventory and opens the gate for the genetic incorporation of other phenylalanine derivatives using engineered pyrrolysyl-tRNA synthetase-tRNA(CUA)(Pyl) pairs.
authors.affiliation: Department of Chemistry, Texas A&M University, College Station, Texas 77843, USA.
authors.forename: Yane-Shih
authors.initials: YS
authors.lastname: Wang
authors.forename: Xinqiang
authors.initials: X
authors.lastname: Fang
authors.forename: Ashley L
authors.initials: AL
authors.lastname: Wallace
authors.forename: Bo
authors.initials: B
authors.lastname: Wu
authors.forename: Wenshe R
authors.initials: WR
authors.lastname: Liu
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics*; D001665:Binding Sites; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism; D005818:Genetic Engineering / Q000379:methods; D049452:Green Fluorescent Proteins / Q000737:chemistry; D008956:Models, Chemical; D009154:Mutation; D009690:Nucleic Acid Conformation; D010455:Peptides / Q000737:chemistry; D010649:Phenylalanine / Q000737:chemistry; D015202:Protein Engineering / Q000379:methods*; D011758:Pyrroles / Q000737:chemistry*; D013379:Substrate Specificity
chemical_list: D010455:Peptides; D011758:Pyrroles; D049452:Green Fluorescent Proteins; D010649:Phenylalanine; D000604:Amino Acyl-tRNA Synthetases