title: Genotyping for cytokine polymorphisms in a Northern Ivory Coast population reveals a high frequency of the heterozygote genotypes for the TNF-α-308G/A SNP.
abstract: Cytokine polymorphisms influence the outcomes of parasitic diseases and vary among populations because of their different evolutionary histories and selective pressures imposed by host-pathogen interactions. In this frame, we investigated the frequencies of TNF-α (-308G/A), TGF-β(1) (codon 10C/T, codon 25C/G) and IL-10 (-1082A/G) SNPs in 133 individuals from Ouangolodougou, a rural village in Northern Ivory Coast, where malaria and other parasitic diseases are endemic. The SNPs alleles were determined by ARMS-PCR methodology. Allele frequencies of the SNPs investigated were as follows: IL 10 -1082G = 0.741 and -1082A = 0.259; TGF-β(1) Codon 10 C = 0.835 and T = 0.165; TGF-β(1) Codon 25 G = 0.782 and C = 0.218. For the TNF-α gene, we found high frequencies of the -308A allele (0.305) and heterozygote genotypes (0.594), with a consequent deviation from the Hardy-Weinberg equilibrium. The high heterozygosity at the TNF-α locus suggests a possible selective advantage of the heterozygote genomes, associated with intermediate levels of TNF-α expression, against the infectious agents endemic in Western Africa.
authors.affiliation: Dipartimento di Biologia Animale e dell'Uomo, Università di Torino, Torino, Italy. alfredo.santovito@unito.it
authors.forename: A
authors.initials: A
authors.lastname: Santovito
authors.forename: P
authors.initials: P
authors.lastname: Cervella
authors.forename: D
authors.initials: D
authors.lastname: Schleicherova
authors.forename: M
authors.initials: M
authors.lastname: Delpero
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D002648:Child; D002675:Child, Preschool; D003062:Codon / Q000235:genetics; D007560:Cote d'Ivoire; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005828:Genetics, Population / Q000379:methods; D015894:Genome, Human; D060005:Genotyping Techniques; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012641:Selection, Genetic; D053773:Transforming Growth Factor beta1 / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*; D055815:Young Adult
chemical_list: D003062:Codon; C508609:IL10 protein, human; D053773:Transforming Growth Factor beta1; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10