title: Novel plasmid and its variant harboring both a bla(NDM-1) gene and type IV secretion system in clinical isolates of Acinetobacter lwoffii.
abstract: The spread of the bla(NDM-1) gene is gaining worldwide attentions. This gene is usually carried by large plasmids and has been discovered in diverse bacteria since it was originally found in Klebsiella pneumoniae. Here we report the complete sequences of a bla(NDM-1)-bearing plasmid, pNDM-BJ01, and its variant, pNDM-BJ02, isolated from clinical Acinetobacter lwoffii strains. The plasmid pNDM-BJ01 is 47.3 kb in size and cannot be classified into any known plasmid incompatibility group, thus representing a novel plasmid with an unknown maintenance mechanism. This plasmid contains both a bla(NDM-1) gene and a type IV secretion system (T4SS) gene cluster. The T4SS is assigned to the P-type T4SS group, which usually encode a short, rigid pilus, and the bla(NDM-1) gene is located within a composite transposon flanked by two insertion elements of ISAba125. Plasmid pNDM-BJ02 is nearly identical to pNDM-BJ01 except that one copy of the ISAba125 element is missing, and it is therefore regarded as a variant of pNDM-BJ01. Sequence alignment indicated that this bla(NDM-1)-containing composite transposon, which can also be captured by other mobile elements, was probably a product of multiple recombination events and can move as a whole by transposition.
authors.affiliation: Department of Laboratory Medicine, The General Hospital of Chinese People’s Armed Police Forces, Beijing, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Hu
authors.forename: Yongfei
authors.initials: Y
authors.lastname: Hu
authors.forename: Yuanlong
authors.initials: Y
authors.lastname: Pan
authors.forename: Hui
authors.initials: H
authors.lastname: Liang
authors.forename: Haiyan
authors.initials: H
authors.lastname: Wang
authors.forename: Xiumei
authors.initials: X
authors.lastname: Wang
authors.forename: Qinfang
authors.initials: Q
authors.lastname: Hao
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Yang
authors.forename: Xi
authors.initials: X
authors.lastname: Yang
authors.forename: Xue
authors.initials: X
authors.lastname: Xiao
authors.forename: Chunguang
authors.initials: C
authors.lastname: Luan
authors.forename: Yi
authors.initials: Y
authors.lastname: Yang
authors.forename: Yujun
authors.initials: Y
authors.lastname: Cui
authors.forename: Ruifu
authors.initials: R
authors.lastname: Yang
authors.forename: George F
authors.initials: GF
authors.lastname: Gao
authors.forename: Yajun
authors.initials: Y
authors.lastname: Song
authors.forename: Baoli
authors.initials: B
authors.lastname: Zhu
mesh_terms: D000150:Acinetobacter / Q000235:genetics*; D000151:Acinetobacter Infections / Q000382:microbiology*; D000328:Adult; D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D019295:Computational Biology; D003227:Conjugation, Genetic; D004251:DNA Transposable Elements / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D005260:Female; D016680:Genome, Bacterial; D006801:Humans; D008875:Middle Aged; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction; D014552:Urinary Tract Infections / Q000382:microbiology; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D001618:beta-Lactamases; C552864:beta-lactamase NDM-1