title: [Research on the extracorporeal cytocompatibility of a composite of HA, carbon fiber and polyetheretherket-one].
abstract: The present research was to study the biocompatibility of a composite of hydroxyapatite (HA), carbon fiber (CF) and polyetheretherket-one (PEEK) by co-culturing with the osteoblasts in vitro. Cell relative growth (RGR) was used as a quantitative assessment for cytotoxicity of the biomaterials by CCK-8. The proliferation index of the co-cultured cells and ALP activity was measured to study the effect of PEEK-HA-CF composites. Morphological properties of the osteoblast cells in vitro were observed by scanning electro-microscopy (SEM). The PEEK-HA-CF materials have no cytotoxicity to osteoblasts. The proliferation index of PEEK-HA-CF was higher than that of Ti alloy group, but these was no significant difference compared to that of control group. The ALP activity was the highest on PEEK-HA-CF composites surface after 7 days. The osteoblast cells co-cultured with the PEEK-HA-CF composite were adhered well to the biomaterial as observed under the SEM. The results suggested that the PEEK-HA-CF composites had good biocompatibility in vitro and might be a novel orthopedic implanted material.
authors.affiliation: Department of Orthopedic Surgery, Shengjing Hospital Affiliated to China Medical University, Shenyang 110004, China. liuxy@sj-hospital.org
authors.forename: Xueyong
authors.initials: X
authors.lastname: Liu
authors.forename: Chunbo
authors.initials: C
authors.lastname: Deng
authors.forename: Jiquan
authors.initials: J
authors.lastname: Liu
authors.forename: Jianjun
authors.initials: J
authors.lastname: Li
authors.forename: Guoxin
authors.initials: G
authors.lastname: Sui
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001577:Benzophenones; D001672:Biocompatible Materials; D002244:Carbon / Q000737:chemistry*; D000077482:Carbon Fiber; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D017886:Durapatite / Q000737:chemistry*; D007659:Ketones / Q000737:chemistry*; D009985:Orthopedics; D010006:Osteoblasts / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers; D019736:Prostheses and Implants; D051381:Rats
chemical_list: D001577:Benzophenones; D001672:Biocompatible Materials; D000077482:Carbon Fiber; D007659:Ketones; D011108:Polymers; C063834:polyetheretherketone; D011092:Polyethylene Glycols; D002244:Carbon; D017886:Durapatite; D000469:Alkaline Phosphatase