title: PET imaging with [18F]AV-45 in an APP/PS1-21 murine model of amyloid plaque deposition.
abstract: Alzheimer's disease (AD), the most common age-related neurodegenerative disorder, is characterized by the accumulation of β-amyloid peptide. In man, [18F]AV-45 with positron emission tomography (PET) is currently studied and used to track in vivo amyloid accumulation. Here, [18F]-AV45-PET was used to visualize amyloid deposition in a transgenic murine model of amyloidosis (APP/PS1-21). Studies were performed ex vivo by autoradiography and in vivo by microPET. Autoradiograms of the brain sections highlighted an increased uptake of [18F]AV-45 in APP/PS1-21 mice compared with age-matched control mice. From 8 months, an intense labeling was observed in cortex, hippocampus, and striatum. The marked accumulation of radiotracer was found in close association with thioflavin S-positive amyloid plaques. The longitudinal microPET assessment, performed from 3 to 12 months of age, demonstrated an increased [18F]AV-45 uptake in APP/PS1-21 compared with control mice. The elevated tracer uptake was increased in association with age. This study opens the possibility of [18F]AV-45, coupled with microPET, to visualize and quantitatively measure amyloid deposits in the brains of living APP/PS1 mice.
authors.affiliation: CEA/DSV/I2BM UMR 6301 ISTCT LDM-TEP, Université de Caen Basse-Normandie, Centre Cyceron, Caen, France.
authors.forename: Géraldine
authors.initials: G
authors.lastname: Poisnel
authors.forename: Martine
authors.initials: M
authors.lastname: Dhilly
authors.forename: Olivier
authors.initials: O
authors.lastname: Moustié
authors.forename: Jérôme
authors.initials: J
authors.lastname: Delamare
authors.forename: Ahmed
authors.initials: A
authors.lastname: Abbas
authors.forename: Denis
authors.initials: D
authors.lastname: Guilloteau
authors.forename: Louisa
authors.initials: L
authors.lastname: Barré
mesh_terms: D000544:Alzheimer Disease / Q000000981:diagnostic imaging / Q000378:metabolism*; D016229:Amyloid beta-Peptides / Q000378:metabolism; D016564:Amyloid beta-Protein Precursor / Q000378:metabolism; D000818:Animals; D001345:Autoradiography / Q000379:methods; D004195:Disease Models, Animal; D005260:Female; D019788:Fluorodeoxyglucose F18; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D058225:Plaque, Amyloid / Q000000981:diagnostic imaging / Q000378:metabolism* / Q000473:pathology; D049268:Positron-Emission Tomography / Q000379:methods*
chemical_list: D016229:Amyloid beta-Peptides; D016564:Amyloid beta-Protein Precursor; D019788:Fluorodeoxyglucose F18