title: Immunohistochemical evidence of cone-based ultraviolet vision in divergent bat species and implications for its evolution.
abstract: We characterized Fos-like expression patterns in the primary visual cortex (V1) by binocular flicking stimulation with UV light to investigate cone-based UV vision in four bat species representing four lineages: Hipposideros armiger and Scotophilus kuhlii, insectivores using constant frequency (CF) or frequency modulation (FM) echolocation, respectively, and Rousettus leschenaultii and Cynopterus sphinx, cave-roosting and tree-roosting fruit bats, respectively. The optic centre processing the visual image, V1, appears more distinctly immunostaining in S. kuhlii and C. sphinx after 1h of UV light stimuli while in H. armiger and R. leschenaultii, staining was no more distinct than in corresponding controls. Our immunohistochemical evidence supports differences in the distribution of cone-based UV vision in the order Chiroptera and supports our earlier postulate that due to possible sensory tradeoffs and roosting ecology, defects in the short wavelength opsin genes have resulted in loss of UV vision in CF but not in FM bats. In addition, fruit bats roosting in caves have lost UV vision but not those roosting in trees. Our results thus confirm that bats are a further mammalian taxon that has retained cone-based UV sensitivity in some species.
authors.affiliation: Institute of Molecular Ecology and Evolution, Institutes for Advanced Interdisciplinary Research, East China Normal University, Zhangshan Road, Shanghai, 200062, PR China.
authors.forename: Fujun
authors.initials: F
authors.lastname: Xuan
authors.forename: Kailiang
authors.initials: K
authors.lastname: Hu
authors.forename: Tengteng
authors.initials: T
authors.lastname: Zhu
authors.forename: Paul
authors.initials: P
authors.lastname: Racey
authors.forename: Xuzhong
authors.initials: X
authors.lastname: Wang
authors.forename: Shuyi
authors.initials: S
authors.lastname: Zhang
authors.forename: Yi
authors.initials: Y
authors.lastname: Sun
mesh_terms: D000220:Adaptation, Biological / Q000502:physiology*; D000818:Animals; D005075:Biological Evolution; D002681:China; D002685:Chiroptera / Q000502:physiology*; D007150:Immunohistochemistry; D017949:Retinal Cone Photoreceptor Cells / Q000502:physiology*; D013045:Species Specificity; D014466:Ultraviolet Rays; D014785:Vision, Ocular / Q000502:physiology*; D014793:Visual Cortex / Q000502:physiology*