title: [Expression of macrophage inflammatory protein-1alpha in EPS and its significance].
abstract: OBJECTIVE
  To detect the mRNA and protein expressions of MIP-1alpha in EPS and determine their significance in the sub-typing of chronic prostatitis.
  
  
  METHODS
  We collected samples of expressed prostatic secretion (EPS) from 50 cases of chronic prostatitis, including 16 cases of chronic bacterial prostatitis (CBP), 23 cases of chronic nonbacterial prostatitis/chronic pelvic pain syndrome (CPPS) (11 CPPS IIIA, 12 CPPS IIIB), and 11 cases of type-IV asymptomatic inflammatory prostatitis (AIP). Another 15 healthy volunteers were included as normal controls. The mRNA and protein levels of MIP-1alpha in EPS were determined by RT-PCR and ELISA, respectively, followed by statistical analysis with SPSS 15.0.
  
  
  RESULTS
  The mRNA expression of MIP-1alpha was markedly higher in the CPPS IIIA and CPPS IIIB groups than in the others (P<0.05). The protein level of MIP-1alpha was (1174.3 +/- 89.2) pg/ml in CPPS IIIA and (842.3 +/- 76.2) pg/ml in CPPS IIIB, significantly higher than (198.0 +/- 37.8) pg/ml in the control, (347.0 +/- 61.6) pg/ml in CBP and (292.0 +/- 56.4) pg/ml in type-IV AIP (P<0.05).
  
  
  CONCLUSIONS
  Determination of mRNA and protein levels of MIP-1alpha in EPS may help the sub-typing and diagnosis of chronic prostatitis.
authors.affiliation: Department of Surgery, The Hospital of Chongqing University of Post and Telecommunication, Chongqing 400065, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zou
authors.forename: Wu-jiang
authors.initials: WJ
authors.lastname: Liu
mesh_terms: D000328:Adult; D000368:Aged; D001826:Body Fluids / Q000378:metabolism*; D016022:Case-Control Studies; D054405:Chemokine CCL3 / Q000378:metabolism*; D002908:Chronic Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D011467:Prostate / Q000378:metabolism; D011472:Prostatitis / Q000378:metabolism*; D055815:Young Adult
chemical_list: C515429:CCL3 protein, human; D054405:Chemokine CCL3