title: Reparation of the myocardium after transplantation of mononuclear bone marrow cells.
abstract: Safety and efficiency of intracoronary transventricular transplantation of autologous mononuclear bone marrow cells in rats with postinfarction cardiosclerosis were studied. The cells migrated to the damaged area and were detected only in the cicatricial tissue; they have fibroblast-like phenotype and some of them were stained with Fapα (marker of reactive fibroblasts). More active proliferation of non-muscular cells and formation and maturation of collagen fibers in the cicatricial tissue were observed after transplantation of mononuclear cells. This led to thickening of the cicatricial wall, but the size of the scar and index of dilatation of the left ventricle remained unchanged. The number and volume density of newly formed blood vessels in the damaged area increased after transplantation, but no labeled cells were seen in the vascular walls. It can be hypothesized that stimulation of neoangiogenesis is mediated by paracrine mechanisms, which also explains improvement of global contractility of the left ventricle (increased contractility index in functional tests). Thus, transplantation of mononuclear bone marrow cells leads to thickening and strengthening of the cicatricial wall, stimulates angiogenesis, and improves global myocardial contractility. However, no morphological signs of reverse remodeling of the left-ventricular myocardium were revealed.
authors.affiliation: Institute of Human Morphology, Russian Academy of Medical Sciences, Moscow, Russia.
authors.forename: Yu P
authors.initials: YP
authors.lastname: Baikova
authors.forename: T Kh
authors.initials: TKh
authors.lastname: Fatkhudinov
authors.forename: G B
authors.initials: GB
authors.lastname: Bol'shakova
authors.forename: T B
authors.initials: TB
authors.lastname: Bukharova
authors.forename: G A
authors.initials: GA
authors.lastname: Slashcheva
authors.forename: O V
authors.initials: OV
authors.lastname: Khokhlova
authors.forename: A N
authors.initials: AN
authors.lastname: Murashev
authors.forename: D V
authors.initials: DV
authors.lastname: Gol'dshtein
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology; D016026:Bone Marrow Transplantation / Q000379:methods*; D006348:Cardiac Surgical Procedures / Q000379:methods*; D002454:Cell Differentiation; D002465:Cell Movement; D049109:Cell Proliferation; D064987:Cell- and Tissue-Based Therapy / Q000379:methods; D009200:Myocardial Contraction / Q000502:physiology; D009203:Myocardial Infarction / Q000473:pathology / Q000503:physiopathology / Q000601:surgery; D009206:Myocardium / Q000473:pathology; D018919:Neovascularization, Physiologic; D051381:Rats; D033581:Stem Cell Transplantation / Q000379:methods*; D014182:Transplantation, Autologous; D016277:Ventricular Function, Left