title: Inhibiting effect of Dorstenia asaroides extracts on cariogenic properties of Streptococcus mutans.
abstract: The aim of this study was to examine the effects of Dorstenia asaroides extracts on cariogenic properties of the most cariogenic bacteria, Streptococcus mutans. Hexane (HFr), ethyl-acetate (EFr) and chloroform (CFr) extracts obtained from D. asaroides rhizomes were submitted to chemical analyses, Minimal Inhibitory Concentrations (MIC), glycolysis assay and S. mutans 12-h-old initial biofilms. Chemical characterization showed that all the extracts present furanocoumarins. The MIC values were 80 (HFr and CFr) and 50 μg/mL (EFr). Acid production by S. mutans cells was significantly disrupted by HFr (12.5 mg/mL), EFr (at 2.5; 6.25 and 12.5 mg/mL) and CFr (at 2.5, 6.25 and 12.5 mg/mL) (p < 0.01). Topical applications of HFr, EFr and CFr significantly reduced the colony forming units of S. mutans biofilms compared with those treated with control group in order to 20, 30 and 25% respectively (p < 0.01). The results of the present study suggest that rhizomes of D. asaroides had inhibitory effects on cariogenic properties of S. mutans.
authors.affiliation: Department of Physics and Chemistry, Faculty of Pharmaceutical Sciences of Ribeirao Preto, University of Sao Paulo-USP, Av. do Café s/n, Monte Alegre, 14040-903 Ribeirão Preto, São Paulo, Brazil.
authors.forename: C E M
authors.initials: CE
authors.lastname: D'Angelis
authors.forename: M F
authors.initials: MF
authors.lastname: Leite
authors.forename: J P B
authors.initials: JP
authors.lastname: Sousa
authors.forename: L
authors.initials: L
authors.lastname: Alonso
authors.forename: A C M
authors.initials: AC
authors.lastname: Polizello
authors.forename: M
authors.initials: M
authors.lastname: Groppo
authors.forename: C P
authors.initials: CP
authors.lastname: Aires
authors.forename: J K
authors.initials: JK
authors.lastname: Bastos
authors.forename: A C C
authors.initials: AC
authors.lastname: Spadaro
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects; D006019:Glycolysis / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D029586:Moraceae / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D013295:Streptococcus mutans / Q000187:drug effects* / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts