title: Effect of protein on the detection of fluoroquinolone residues in fish meat.
abstract: Using fish serum albumin (FSA) as the model protein, molecular fluorescence spectrometry and high-performance liquid chromatography (HPLC) were applied to study the effect of protein on the extraction of fluoroquinolone (FQ) residues in fish meat. There was a strong interaction between FQs and protein through hydrogen bonds, which could be broken as protein degenerated with 60-100% (v/v) acetonitrile acid solution, and FQs bound with protein were released in various degrees. On the basis of the results, a novel sample preparation procedure loosely based on QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) methodology was developed for the determination of FQ residues in fish muscle samples, using 90% (v/v) acetonitrile acid solution as the extractant, combined with a dispersive solid-phase extraction (DSPE) cleanup step. Mean recoveries of four FQs from spiked samples at a concentration range of 50-200 ng g(-1) were 73.3-95.9% with relative standard deviations (RSD) lower than 10.7%.
authors.affiliation: College of Chemistry and Chemical Engineering, Yantai University, Yantai, People's Republic of China.
authors.forename: Huifang
authors.initials: H
authors.lastname: Li
authors.forename: Jungang
authors.initials: J
authors.lastname: Yin
authors.forename: Yongming
authors.initials: Y
authors.lastname: Liu
authors.forename: Jian
authors.initials: J
authors.lastname: Shang
mesh_terms: D000097:Acetonitriles; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002347:Carps; D002851:Chromatography, High Pressure Liquid; D029941:Fish Proteins / Q000378:metabolism*; D024841:Fluoroquinolones / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D006860:Hydrogen Bonding; D008460:Meat / Q000032:analysis*; D009132:Muscles / Q000737:chemistry; D015203:Reproducibility of Results; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
chemical_list: D000097:Acetonitriles; D000900:Anti-Bacterial Agents; D029941:Fish Proteins; D024841:Fluoroquinolones; D012709:Serum Albumin; C032159:acetonitrile