title: The isolation of a new S-methyl benzothioate compound from a marine-derived Streptomyces sp.
abstract: The application of an HPLC bioactivity profiling/microtiter plate technique in conjunction with microprobe NMR instrumentation and access to the AntiMarin database has led to the isolation of a new 1. In this example, 1 was isolated from a cytotoxic fraction of an extract obtained from marine-derived Streptomyces sp. cultured on Starch Casein Agar (SCA) medium. The 1D and 2D (1)H NMR and ESIMS data obtained from 20 μg of compound 1 fully defined the structure. The known 2 was also isolated and readily dereplicated using this approach.
authors.affiliation: Faculty of Food Science and Technology, Universiti Putra Malaysia, Selangor, Serdang, Malaysia. norainy@food.upm.edu.my
authors.forename: Nor Ainy
authors.initials: NA
authors.lastname: Mahyudin
authors.forename: John W
authors.initials: JW
authors.lastname: Blunt
authors.forename: Anthony L J
authors.initials: AL
authors.lastname: Cole
authors.forename: Murray H G
authors.initials: MH
authors.lastname: Munro
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008386:Marine Biology; D051379:Mice; D015394:Molecular Structure; D011763:Pyrrolizidine Alkaloids / Q000737:chemistry / Q000302:isolation & purification*; D013302:Streptomyces / Q000737:chemistry*; D013457:Sulfur Compounds / Q000138:chemical synthesis / Q000302:isolation & purification; D014871:Water Microbiology
chemical_list: D011763:Pyrrolizidine Alkaloids; C572885:S-methyl 2,4-dihydroxy-6-isopropyl-3,5-dimethylbenzothioate; D013457:Sulfur Compounds; C025548:bohemamine