title: Electrodeposition of pronectin for titanium to augment gingival epithelium adhesion.
abstract: This paper is one trial of surface modification of titanium with pronectin F+ (PN) of an artificial protein to enhance gingival adhesion. Titanium plates were electrodeposited in the PN solution to prepare PN-electrodeposited titanium plates. When PN detachment from the PN-electrodeposited titanium plates was investigated, no detachment was observed, in contrast to the case of titanium plates simply coated with PN. A cell culture experiment demonstrated that electrodeposited PN had an inherent ability to enhance the initial attachment of gingival epithelial cells. The PN-electrodeposited titanium plates were implanted between the gingival epithelium and the underlying bone tissue of rabbits to evaluate epithelial growth on the plates and their gingival adhesion. Non-treated and PN-coated titanium plates were used as controls. PN electrodeposition enhanced epithelial growth and adhesion of titanium plates to a significantly great extent compared with PN-coated plates. These findings demonstrate that PN electrodeposition is a promising method to enhance epithelium adhesion onto a titanium surface.
authors.affiliation: Katsura Research Laboratory Bio and Medical Products Research Department, Sanyo Chemical Industries Ltd, Kyoto, Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Kawabata
authors.forename: Kazunari
authors.initials: K
authors.lastname: Asano
authors.forename: Atsuko
authors.initials: A
authors.lastname: Miyazawa
authors.forename: Tazuko
authors.initials: T
authors.lastname: Satoh
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Tabata
mesh_terms: D000327:Adsorption; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D004595:Electroplating / Q000379:methods*; D004847:Epithelial Cells / Q000187:drug effects / Q000473:pathology; D004848:Epithelium / Q000187:drug effects / Q000473:pathology*; D005353:Fibronectins / Q000494:pharmacology*; D005881:Gingiva / Q000473:pathology*; D011817:Rabbits; D011994:Recombinant Proteins / Q000494:pharmacology; D013997:Time Factors; D014025:Titanium / Q000494:pharmacology*
chemical_list: D005353:Fibronectins; D011994:Recombinant Proteins; C070367:ProNectin F; D014025:Titanium