title: Mast cells mediate Pseudomonas aeruginosa lipopolysaccharide-induced lung inflammation in rat.
abstract: Activated mast cells have been demonstrated to play a pivotal role in Pseudomonas aeruginosa lung infections. However, there is no report about the involvement of mast cells in P. aeruginosa lipopolysaccharide (LPS)-induced lung inflammation. This study aimed at evaluating the role of mast cells in P. aeruginosa LPS-induced lung inflammation in rats. Mast cells stabilization was carried out by intraperitoneal injections of cromolyn. Lung inflammation was induced by the intratracheal instillation of P. aeruginosa LPS (5 μg/kg bw) and inflammatory status was evaluated 4 h post-LPS instillation. We found that activated mast cells could constitute a pivotal source of several inflammatory cytokines, including TNF-α, IL-1β, and IL-6. These cells might regulate polymorphonuclear neutrophil (PMN) recruitment and be implicated in the alteration of alveolar-capillary permeability via the release of TNF-α and IL-1β. We also detected that activated mast cells could be involved in the alteration of the expression of two epithelial tight junction proteins (claudin-1 and occludin) during the acute phase of inflammation. Our results suggest that activated mast cells might play a critical role in P. aeruginosa LPS-induced lung inflammation. Therefore, mast cell stabilization may be a potential novel approach for the prevention and treatment of P. aeruginosa-induced lung infections.
authors.affiliation: Peritox Laboratory, EA4285-UMI 01, Faculty of Medicine, Picardy Jules Verne University, 3 rue des Louvels, 80036, Amiens, France.
authors.forename: B V
authors.initials: BV
authors.lastname: Lê
authors.forename: H
authors.initials: H
authors.lastname: Khorsi-Cauet
authors.forename: V
authors.initials: V
authors.lastname: Bach
authors.forename: J
authors.initials: J
authors.lastname: Gay-Quéheillard
mesh_terms: D000210:Acute-Phase Reaction / Q000276:immunology / Q000473:pathology; D000818:Animals; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D006801:Humans; D008070:Lipopolysaccharides / Q000276:immunology* / Q000633:toxicity*; D008297:Male; D008407:Mast Cells / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology; D011014:Pneumonia / Q000276:immunology* / Q000473:pathology; D011550:Pseudomonas aeruginosa / Q000276:immunology / Q000472:pathogenicity*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D016207:Cytokines; D008070:Lipopolysaccharides