title: Association of Thr420Lys polymorphism in DBP gene with fat-soluble vitamins and low radial bone mineral density in postmenopausal Thai women.
abstract: OBJECTIVE
  To investigate the genetic markers for osteoporosis bone mineral density by the genotyping of rs7041, rs4588 and rs1352845 in the DBP gene with either bone mineral density or serum 25-hydroxycholecalciferol, retinol and α-tocopherol, among 365 postmenopausal Thai women.
  
  
  METHODS
  The DBP genotypes were analyzed by a PCR restriction fragment-length polymorphism method. Serum 25-hydroxycholecalciferol was assessed using a commercial chemiluminescent immunoassay. Serum retinol and α-tocopherol were measured by reverse-phase high-performance liquid chromatography.
  
  
  RESULTS
  After adjustment for age >50 years, elder Thai subjects with low BMI (≤25 kg/m(2)) and carrying the rs4588 CC genotype had a higher risk of radial bone mineral density osteoporosis (odds ratio: 6.29; p = 0.048). The rs1352845 genotype also had a statistical association with total hip bone mineral density; however, it disappeared after adjustment for age and BMI. No association was found in fat-soluble vitamins with bone mineral density.
  
  
  CONCLUSIONS
  DBP genotypes may influence the osteoporosis bone mineral density in postmenopausal Thai women.
authors.affiliation: Department of Tropical Nutrition & Food Science, Faculty of Tropical Medicine, Mahidol University, Ratchathewi, Bangkok, Thailand.
authors.forename: Chaowanee
authors.initials: C
authors.lastname: Chupeerach
authors.forename: Anchalee
authors.initials: A
authors.lastname: Tungtrongchitr
authors.forename: Benjaluck
authors.initials: B
authors.lastname: Phonrat
authors.forename: Florian J
authors.initials: FJ
authors.lastname: Schweigert
authors.forename: Rungsunn
authors.initials: R
authors.lastname: Tungtrongchitr
authors.forename: Sangchai
authors.initials: S
authors.lastname: Preutthipan
mesh_terms: D000368:Aged; D015519:Bone Density; D005223:Fats / Q000737:chemistry*; D005260:Female; D005819:Genetic Markers / Q000235:genetics; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D010024:Osteoporosis / Q000097:blood / Q000235:genetics / Q000503:physiopathology; D020641:Polymorphism, Single Nucleotide; D017698:Postmenopause / Q000097:blood / Q000235:genetics* / Q000502:physiology; D012995:Solubility; D013785:Thailand; D014809:Vitamin D-Binding Protein / Q000235:genetics*; D014815:Vitamins / Q000097:blood* / Q000737:chemistry*
chemical_list: D005223:Fats; D005819:Genetic Markers; D014809:Vitamin D-Binding Protein; D014815:Vitamins