title: Study of degradation behavior of baicalin during simulative blood sample processing with solid phase extraction technology and identification of the degradation product.
abstract: In the present study, an LC study of degradation behavior of baicalin under various buffer solutions (pH 5.0, 7.0, 9.0) was carried out during simulative blood sample processing with solid phase extraction technology. The results show that during simulative blood sample processing with solid phase extraction technology, baicalin was apt to undergo degradation in buffer solutions (pH 7.0, 9.0) but was stable in buffer solutions (pH 5.0). Primarily, maximum degradation product was achieved by subjecting baicalin to drug-free blood and buffer solution (pH 7.0), standing for 30 min, then rinsing with buffer solution (pH 7.0) followed by elution with methanol from Solid Phase Extraction cartridge, and keeping the methanol eluate at 50 °C for more than 11 hs. The product was characterized as negletein through LC-MS fragmentation and ¹H-NMR, ¹³C-NMR spectra studies.
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, Xixia District, Nanjing, China.
authors.forename: Lihong
authors.initials: L
authors.lastname: Chen
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.forename: Yuping
authors.initials: Y
authors.lastname: Tang
mesh_terms: D000818:Animals; D002021:Buffers; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D047309:Flavones / Q000097:blood* / Q000737:chemistry; D005419:Flavonoids / Q000097:blood* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D013058:Mass Spectrometry; D000432:Methanol / Q000737:chemistry; D015394:Molecular Structure; D051381:Rats; D017208:Rats, Wistar; D052616:Solid Phase Extraction; D012997:Solvents / Q000737:chemistry; D013696:Temperature; D013997:Time Factors
chemical_list: D002021:Buffers; D047309:Flavones; D005419:Flavonoids; D012997:Solvents; C507557:negletein; C038044:baicalin; D000432:Methanol