title: Cyclic interconversion of methionine containing peptide between oxidized and reduced phases monitored by reversed-phase HPLC and ESI-MS/MS.
abstract: The cyclic oxidation and reduction of methionine (Met) containing peptides and proteins play important roles in biological system. This work was contributed to analysis the cyclic oxidation and reduction processes of a methionine containing peptide which is very likely to relate in the cell signal transduction pathways. To mimic the biological oxidation condition, hydrogen peroxide was used as the reactive oxygen species to oxidize the peptide. Reversed-phase high-performance liquid chromatography and mass spectrometry were employed to monitor the reactions and characterize the structural changes of the products. A rapid reduction procedure was developed by simply using KI as the reductant, which is green and highly efficient. By investigation of the cyclic oxidation and reduction process, our work provides a new perspective to study the function and mechanism of Met containing peptides and proteins during cell signaling processes as well as diseases.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, CAS Key Lab of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Jin
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Huang
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Xie
authors.forename: Wenbing
authors.initials: W
authors.lastname: Hu
authors.forename: Fuyi
authors.initials: F
authors.lastname: Wang
authors.forename: Guoquan
authors.initials: G
authors.lastname: Liu
authors.forename: Rui
authors.initials: R
authors.lastname: Zhao
mesh_terms: D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry*; D008715:Methionine / Q000737:chemistry*; D008969:Molecular Sequence Data; D015513:Oncogene Proteins / Q000737:chemistry*; D010084:Oxidation-Reduction; D010455:Peptides / Q000737:chemistry*; D011193:Potassium Iodide / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000737:chemistry; D060327:Solid-Phase Synthesis Techniques; D012996:Solutions; D021241:Spectrometry, Mass, Electrospray Ionization; D013237:Stereoisomerism; D053719:Tandem Mass Spectrometry
chemical_list: C477083:LAPTM4B protein, human; D008565:Membrane Proteins; D015513:Oncogene Proteins; D010455:Peptides; D017382:Reactive Oxygen Species; D012996:Solutions; D011193:Potassium Iodide; D008715:Methionine; D006861:Hydrogen Peroxide