title: Clearance of amyloid-β protein deposits in transgenic mice following focal cerebral ischemia.
abstract: OBJECTIVE
  There is increased amyloid-β protein precursor (AβPP) expression and amyloid-β protein (Aβ) production in the brain shortly following cerebral ischemic stroke in rodent models. It has been postulated that this may seed amyloid deposition in the brain. On the other hand, it remains unclear how cerebral ischemia affects preexisting Aβ deposits in the brain. Here we determine the consequences of focal ischemic stroke on existing Aβ pathology in Tg-SwDI transgenic mice.
  
  
  METHODS
  At 12 months of age, Tg-SwDI mice were subjected to photo-induced focal cerebral ischemia in one hemisphere. One, 7, or 21 days after lesioning, the amount of deposited Aβ in the ischemic and control hemispheres was measured using ELISA. Image analysis was used to visualize deposited Aβ and the presence of microglia/macrophages.
  
  
  RESULTS
  After 7 days, and further after 21 days, there was a dramatic reduction in the amount of deposited Aβ and increased presence of microglia/macrophages in the ischemic hemisphere of the mice.
  
  
  CONCLUSIONS
  Focal cerebral ischemia leads to clearance of deposited Aβ in Tg-SwDI mice starting at 7 days with almost complete removal in the ischemic area by 21 days. The delayed clearance of Aβ following focal cerebral ischemia may involve the infiltration of activated neuroinflammatory cells.
authors.affiliation: Department of Neurosurgery and Medicine, Stony Brook University, Stony Brook, NY 11794-8122, USA. William.VanNostrand@sbumed.org
authors.forename: W E
authors.initials: WE
authors.lastname: Van Nostrand
authors.forename: J
authors.initials: J
authors.lastname: Davis
authors.forename: M L
authors.initials: ML
authors.lastname: Previti
authors.forename: F
authors.initials: F
authors.lastname: Xu
mesh_terms: D016229:Amyloid beta-Peptides / Q000378:metabolism*; D016564:Amyloid beta-Protein Precursor / Q000235:genetics; D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D007839:Functional Laterality; D006801:Humans; D008264:Macrophages / Q000473:pathology; D051379:Mice; D008822:Mice, Transgenic; D017628:Microglia / Q000473:pathology; D009154:Mutation / Q000235:genetics; D013997:Time Factors
chemical_list: D016229:Amyloid beta-Peptides; D016564:Amyloid beta-Protein Precursor