title: INO1 induction requires chromatin remodelers Ino80p and Snf2p but not the histone acetylases.
abstract: Transcriptional co-activators contribute to gene expression through different mechanisms. We used various biochemical tools available for Saccharomyces cerevisiae to examine the mechanism of INO1 expression. INO1 encodes inositol-3-phosphate synthase, which catalyzes the rate-limiting step in the synthesis of inositol, a key player in phospholipid biosynthesis. Herein, we had demonstrated that the recruitment of histone acetylases Gcn5p and Esa1p mainly relied on the presence of transcriptional activator Ino2p during INO1 activation. However, the presence of the chromatin remodelers, Ino80p and Snf2p, may contribute to the additive effect of Gcn5p recruitment. We also showed that the recruitment of chromatin remodelers, Ino80p and Snf2p, is independent of the presence of histone acetylases. Furthermore, INO1 expression can be activated exclusively by the activator and chromatin remodelers, suggesting a dispensable role of histone acetylases in INO1 induction. Therefore, our data provide a mechanism for cross talk within transcriptional co-activators during INO1 activation.
authors.affiliation: Department of Biology, College of Staten Island, City University of New York, 2800 Victory Blvd., Staten Island, NY 10314, United States.
authors.forename: Paulina
authors.initials: P
authors.lastname: Konarzewska
authors.forename: Michelle
authors.initials: M
authors.lastname: Esposito
authors.forename: Chang-Hui
authors.initials: CH
authors.lastname: Shen
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism*; D042002:Chromatin Assembly and Disassembly; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D051548:Histone Acetyltransferases / Q000235:genetics / Q000378:metabolism; D047468:Immunoprecipitation; D007296:Myo-Inositol-1-Phosphate Synthase / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation; D000099224:p300-CBP-Associated Factor
chemical_list: D000251:Adenosine Triphosphatases; D051548:Histone Acetyltransferases; D007296:Myo-Inositol-1-Phosphate Synthase; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C483404:INO80 complex, S cerevisiae; C496551:Esa1 protein, S cerevisiae; C501392:GCN5 protein, S cerevisiae; D000099224:p300-CBP-Associated Factor; C499333:SNF2 protein, S cerevisiae; C519641:INO1 protein, S cerevisiae