title: Re-electrospraying splash-landed proteins and nanoparticles.
abstract: FITC-albumin, Lsr-F, or fluorescent polystyrene latex particles were electrosprayed from aqueous buffer and subjected to dispersion by differential electrical mobility at atmospheric pressure. A resulting narrow size cut of singly charged molecular ions or particles was passed through a condensation growth tube collector to create a flow stream of small water droplets, each carrying a single ion or particle. The droplets were splash landed (impacted) onto a solid or liquid temperature controlled surface. Small pools of droplets containing size-selected particles, FITC-albumin, or Lsr-F were recovered, re-electrosprayed, and, when analyzed a second time by differential electrical mobility, showed increased homogeneity. Transmission electron microscopy (TEM) analysis of the size-selected Lsr-F sample corroborated the mobility observation.
authors.affiliation: Physics and Life Sciences, Biology and Biotechnology Division, Lawrence Livermore National Laboratory, P.O. Box 808, Livermore, California 94551, USA. benner2@llnl.gov
authors.forename: W Henry
authors.initials: WH
authors.lastname: Benner
authors.forename: Gregory S
authors.initials: GS
authors.lastname: Lewis
authors.forename: Susanne V
authors.initials: SV
authors.lastname: Hering
authors.forename: Brent
authors.initials: B
authors.lastname: Selgelke
authors.forename: Michelle
authors.initials: M
authors.lastname: Corzett
authors.forename: James E
authors.initials: JE
authors.lastname: Evans
authors.forename: Felice C
authors.initials: FC
authors.lastname: Lightstone
mesh_terms: D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives* / Q000737:chemistry; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011137:Polystyrenes / Q000737:chemistry; D012709:Serum Albumin / Q000737:chemistry*; D013499:Surface Properties; D013696:Temperature
chemical_list: C518288:FITC-albumin; D011137:Polystyrenes; D012709:Serum Albumin; D016650:Fluorescein-5-isothiocyanate