title: Assessment of bactericidal effects of quaternary ammonium-based antibacterial monomers in combination with colloidal platinum nanoparticles.
abstract: Pretreatment of dentin using colloidal platinum nanoparticles (CPtN) can enhance the bond strength of dentin adhesives. However, the combination of CPtN, which is negatively charged, with cationic monomer-containing adhesive may reduce the antibacterial activity of the original material. Thus, the purpose of this study was to assess the effect of CPtN on the bactericidal activity of two cationic antibacterial monomers, 12-methacryloyloxydodecylpyridinium bromide (MDPB) and methacryloxylethyl cetyl dimethyl ammonium chloride (DMAE-CB). The rapid killing effects of the two monomers against planktonic or attached Streptococcus mutans in the presence or absence of CPtN were examined by viable cell counts. The measurement of minimum inhibitory and bactericidal concentrations demonstrated that CPtN up to 2.5 mM has no antibacterial activity. In the absence of CPtN, rapid killing of both planktonic and attached Streptococcus mutans were achieved by the two cationic monomers. Combination with 0.1 mM CPtN did not reduce the bactericidal effects of the two monomers, indicating that CPtN may be used as a pretreatment with antibacterial adhesives.
authors.affiliation: Department of Prosthodontics, School of Stomatology, Fourth Military Medical University, Xi’an, 710032, PR China.
authors.forename: Sai
authors.initials: S
authors.lastname: Ma
authors.forename: Naomi
authors.initials: N
authors.lastname: Izutani
authors.forename: Satoshi
authors.initials: S
authors.lastname: Imazato
authors.forename: Ji-Hua
authors.initials: JH
authors.lastname: Chen
authors.forename: Wakako
authors.initials: W
authors.lastname: Kiba
authors.forename: Ranna
authors.initials: R
authors.lastname: Yoshikawa
authors.forename: Kahoru
authors.initials: K
authors.lastname: Takeda
authors.forename: Haruaki
authors.initials: H
authors.lastname: Kitagawa
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Ebisu
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D058491:Bacterial Load / Q000187:drug effects; D018441:Biofilms / Q000187:drug effects; D001840:Dental Bonding / Q000379:methods; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D004347:Drug Interactions; D006801:Humans; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D018613:Microscopy, Confocal; D053758:Nanoparticles / Q000737:chemistry*; D010984:Platinum / Q000737:chemistry*; D011726:Pyridinium Compounds / Q000737:chemistry / Q000494:pharmacology; D000644:Quaternary Ammonium Compounds / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D013295:Streptococcus mutans / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D017220:Dentin-Bonding Agents; D008689:Methacrylates; D011726:Pyridinium Compounds; D000644:Quaternary Ammonium Compounds; C540902:methacryloxylethyl cetyl dimethyl ammonium; C089392:methacryloyloxydodecylpyridinium bromide; D010984:Platinum