title: Down-regulation of fecal miR-143 and miR-145 as potential markers for colorectal cancer.
abstract: OBJECTIVE
  To detect 4 MicroRNA (miRNA) in the stool samples of colorectal cancer (CRC) patients to determine whether these miRNAs could be biomarkers in CRC screening or treatment.
  
  
  METHODS
  A retrospective comparison study was carried out in the Department of Colorectal Surgery,    Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China from September 2009 to March 2011. We detected 4 miRNAs (miR-143, miR-145, miR-21, and miR-106a) in the stool samples of 38 CRC patients and 13 healthy individuals. Total RNA from the stool samples was extracted using the EZNA TM stool RNA kit R6828-01. The miRNA quantification was carried out using TaqMan miRNA assays and the TaqMan Gene Expression Master Mix.
  
  
  RESULTS
  The expression levels of miR-143 and miR-145 in the stool of the CRC patients were lower than in those of the healthy persons (p<0.005, median of 2-∆∆ct). No statistically significant difference was found in the expression levels of both miR-21 and miR-106a between the stool of CRC patients and those of the healthy persons (p>0.05).
  
  
  CONCLUSIONS
  The detection of fecal miRNAs is a potential method for CRC diagnosis or screening. Particularly, the down-regulation of fecal miR-143 and miR-145 could be a potential marker for CRC.
authors.affiliation: Department of Colorectal Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
authors.forename: Jin-Ming
authors.initials: JM
authors.lastname: Li
authors.forename: Rong-Hua
authors.initials: RH
authors.lastname: Zhao
authors.forename: Shao-Tang
authors.initials: ST
authors.lastname: Li
authors.forename: Cai-Xia
authors.initials: CX
authors.lastname: Xie
authors.forename: Hong-Hua
authors.initials: HH
authors.lastname: Jiang
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Ding
authors.forename: Peng
authors.initials: P
authors.lastname: Du
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Ming
authors.initials: M
authors.lastname: Yang
authors.forename: Long
authors.initials: L
authors.lastname: Cui
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000378:metabolism*; D015536:Down-Regulation; D005243:Feces; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D012189:Retrospective Studies
chemical_list: D014408:Biomarkers, Tumor; C542359:MIRN143 microRNA, human; C541010:MIRN145 microRNA, human; D035683:MicroRNAs