title: Osteogenic effect of low intensity pulsed ultrasound on rat adipose-derived stem cells in vitro.
abstract: The osteogenic in vitro effect of low intensity pulsed ultrasound (LIPUS) on SD rat adipose-derived stem cells (ADSCs) was investigated. Rat ADSCs underwent LIPUS (intensity=100 mW/cm(2)) or sham exposure for 8 min per treatment once everyday in vitro, and then the alkaline phosphatase (ALP) activity and mineralized nodule formation were assessed to evaluate the osteogenic effect of LIPUS on ADSCs. To further explore the underlying mechanism, the osteogenic-related gene mRNA expression was determined by using reverse transcriptase-polymerase chain reaction (RT-PCR) at 1st, 3rd, 5th, 7th day after exposure repectively. Westen blot was used to evaluate the protein expression levels of two osteogenic differentiation associated genes at 7th and 14th day repectively. It was found that ALP activity was increased after LIPUS exposure and LIPUS resulted in mineralized nodule formation of ADSCs in vitro. LIPUS-treated ADSCs displayed higher mRNA expression levels of runt-related transcription factor 2 (Runx2), osteocalcin (OCN), ALP and bone sialoprotein (BSP) genes than controls, and the protein levels of Runx2 and BSP were also increased. The results suggested that LIPUS may induce the osteogenic differentiation of ADSCs in vitro.
authors.affiliation: Department of Orthopedics Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.|Department of Orthopedics, the Third Affiliated Hospital, Anhui Medical Univeristy, Hefei, 230031, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Jiang
authors.affiliation: Department of Rehabilitation Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. yyxutao@263.net.
authors.forename: Tao
authors.initials: T
authors.lastname: Xu
authors.affiliation: Department of Orthopedics Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors.forename: Fengjing
authors.initials: F
authors.lastname: Gu
authors.affiliation: Department of Orthopedics Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors.forename: Anmin
authors.initials: A
authors.lastname: Chen
authors.affiliation: Department of Orthopedics Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors.forename: Zhengzheng
authors.initials: Z
authors.lastname: Xiao
authors.affiliation: Department of Orthopedics Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors.forename: Di
authors.initials: D
authors.lastname: Zhang
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000502:physiology / Q000528:radiation effects; D000818:Animals; D002478:Cells, Cultured; D019220:High-Energy Shock Waves; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology / Q000528:radiation effects; D010012:Osteogenesis / Q000502:physiology* / Q000528:radiation effects; D011829:Radiation Dosage; D051381:Rats; D017207:Rats, Sprague-Dawley; D013010:Sonication / Q000379:methods*; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology / Q000528:radiation effects