title: Food assimilated by two  sympatric populations of the brown planthopper Nilaparvata lugens (Delphacidae)  feeding on different host plants contaminates insect DNA detected by RAPD-PCR  analysis.
abstract: Contamination  of insect DNA for RAPD-PCR analysis can be a problem because many primers are  non-specific and DNA from parasites or gut contents may be simultaneously  extracted along with that of the insect. We measured the quantity of food  ingested and assimilated by two sympatric populations of brown planthopper  (BPH), Nilaparvata lugens, one from rice and the other from Leersia  hexandra (Poaceae), a wetland forage grass, and we also investigated  whether host plant DNA contaminates that of herbivore insects in extractions of  whole insects. Ingestion and assimilation of food were reduced significantly  when individuals derived from one host plant were caged on the other species.  The bands, OPA3 (1.25), OPD3 (1.10), OPD3 (0.80), OPD3 (0.60), pUC/M13F (0.35),  pUC/M13F (0.20), BOXAIR (0.50), peh#3 (0.50), and peh#3 (0.17) were found in  both rice-infesting populations of brown planthopper and its host plant (rice).  Similarly, the bands, OPA4 (1.00), OPB10 (0.70), OPD3 (0.90), OPD3 (0.80), OPD3  (0.60), pUC/ M13F (0.35), pUC/M13F (0.20), and BOXAIR (0.50) were found in both Leersia-infesting populations of brown planthopper and the host plant.  So, it is clear that the DNA bands amplified in the host plants were also found  in the extracts from the insects feeding on them.
authors.affiliation: Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, Selangor, Malaysia. alatif1965@yahoo.com
authors.forename: M A
authors.initials: MA
authors.lastname: Latif
authors.forename: M Y
authors.initials: MY
authors.lastname: Omar
authors.forename: S G
authors.initials: SG
authors.lastname: Tan
authors.forename: S S
authors.initials: SS
authors.lastname: Siraj
authors.forename: M E
authors.initials: ME
authors.lastname: Ali
authors.forename: M Y
authors.initials: MY
authors.lastname: Rafii
mesh_terms: D000818:Animals; D059018:DNA Contamination; D018744:DNA, Plant / Q000235:genetics*; D004435:Eating; D006430:Hemiptera / Q000235:genetics*; D060434:Herbivory; D010944:Plants / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods
chemical_list: D018744:DNA, Plant