title: Rolling circle amplification-templated DNA nanotubes show increased stability and cell penetration ability.
abstract: DNA nanotubes hold promise as scaffolds for protein organization, as templates of nanowires and photonic systems, and as drug delivery vehicles. We present a new DNA-economic strategy for the construction of DNA nanotubes with a backbone produced by rolling circle amplification (RCA), which results in increased stability and templated length. These nanotubes are more resistant to nuclease degradation, capable of entering human cervical cancer (HeLa) cells with significantly increased uptake over double-stranded DNA, and are amenable to encapsulation and release behavior. As such, they represent a potentially unique platform for the development of cell probes, drug delivery, and imaging tools.
authors.affiliation: Department of Chemistry, McGill University, 801 Sherbrooke Street West, Montreal, QC H3A 2K6 Canada.
authors.forename: Graham D
authors.initials: GD
authors.lastname: Hamblin
authors.forename: Karina M M
authors.initials: KM
authors.lastname: Carneiro
authors.forename: Johans F
authors.initials: JF
authors.lastname: Fakhoury
authors.forename: Katherine E
authors.initials: KE
authors.lastname: Bujold
authors.forename: Hanadi F
authors.initials: HF
authors.lastname: Sleiman
mesh_terms: D004247:DNA / Q000737:chemistry*; D017931:DNA Primers; D004259:DNA-Directed DNA Polymerase / Q000235:genetics; D003851:Deoxyribonucleases / Q000378:metabolism; D016503:Drug Delivery Systems; D005784:Gene Amplification; D006367:HeLa Cells; D006801:Humans; D009154:Mutation; D053761:Nanocomposites / Q000737:chemistry; D043942:Nanotubes / Q000737:chemistry*; D021141:Nucleic Acid Amplification Techniques; D015347:RNA Probes; D013698:Templates, Genetic
chemical_list: D017931:DNA Primers; D015347:RNA Probes; D004247:DNA; D004259:DNA-Directed DNA Polymerase; D003851:Deoxyribonucleases