title: Gold nanoparticles as a label-free probe for the detection of amyloidogenic protein.
abstract: Because amyloidogenic proteins, such as prion protein, β-amyloid peptide and α-synuclein, are associated with a variety of diseases, methods for their detection are important. Recombinant prion protein (rPrP) can selectively induce aggregation of dihydrolipoic acid capped gold nanoparticles (DHLA-AuNPs), which reduces the absorbance of the DHLA-AuNPs and changes their color from red to blue. These changes were used for label-free qualitative and quantitative detection of amyloidogenic protein. The addition of NaCl improved the detection sensitivity considerably, and the detection limit was as low as 33 pmol/L.
authors.affiliation: College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
authors.forename: Hai-Jie
authors.initials: HJ
authors.lastname: Zhang
authors.forename: Hu-Zhi
authors.initials: HZ
authors.lastname: Zheng
authors.forename: Yi-Juan
authors.initials: YJ
authors.lastname: Long
authors.forename: Geng-Fu
authors.initials: GF
authors.lastname: Xiao
authors.forename: Ling-Yan
authors.initials: LY
authors.lastname: Zhang
authors.forename: Qin-Long
authors.initials: QL
authors.lastname: Wang
authors.forename: Mei
authors.initials: M
authors.lastname: Gao
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Bai
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D002417:Cattle; D003116:Color; D004926:Escherichia coli / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D011328:Prions / Q000097:blood*; D012965:Sodium Chloride / Q000737:chemistry; D013053:Spectrophotometry; D008063:Thioctic Acid / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: D011328:Prions; D012965:Sodium Chloride; D008063:Thioctic Acid; D006046:Gold; C015144:dihydrolipoic acid