title: Flavonoid and cardenolide glycosides and a pentacyclic triterpene from the leaves of Nerium oleander and evaluation of cytotoxicity.
abstract: A pentacyclic triterpene, oleanderocioic acid, two flavonoidal glycosides, quercetin-5-O-[α-L-rhamnopyranosyl-(1→6)]-β-D-glucopyranoside and kaempferol-5-O-[α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranoside, and a cardenolide, oleandigoside, together with 11 known compounds, were isolated from the leaves of Nerium oleander. Their structures were elucidated on the basis of spectroscopic analysis. The growth inhibitory and cytotoxic activities of eight compounds were evaluated against the MCF-7 human breast cancer cell line using a sulforhodamine B assay. Three compounds, oleandrin, odoroside A and B were further assayed using a panel of 57 human cancer cell lines.
authors.affiliation: HEJ Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan. binahej@yahoo.com
authors.forename: Bina Shaheen
authors.initials: BS
authors.lastname: Siddiqui
authors.forename: Nasima
authors.initials: N
authors.lastname: Khatoon
authors.forename: Sabira
authors.initials: S
authors.lastname: Begum
authors.forename: Ahsana Dar
authors.initials: AD
authors.lastname: Farooq
authors.forename: Kehkashan
authors.initials: K
authors.lastname: Qamar
authors.forename: Huma Aslam
authors.initials: HA
authors.lastname: Bhatti
authors.forename: Syed Kashif
authors.initials: SK
authors.lastname: Ali
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D002298:Cardenolides / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005419:Flavonoids / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D006027:Glycosides / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D006801:Humans; D061986:MCF-7 Cells; D030007:Nerium / Q000737:chemistry* / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular; D053978:Pentacyclic Triterpenes / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D002298:Cardenolides; D005419:Flavonoids; D006027:Glycosides; D053978:Pentacyclic Triterpenes; D010936:Plant Extracts