title: Evaluation of orexin A activity on LH and FSH release from primary culture pituitary cells in immature and mature female rats.
abstract: OBJECTIVE
  Orexin A (OxA) is a regulatory neuropeptide which is involved in the control of various autonomic and neuroendocrine functions. It regulates sleep-wake cycle, food intake and modulates the hypothalamic and pituitary hormones secretion. Orexin A acts through two types of receptors, which proved to exist in the pituitary. This may indicate the possibility of direct action of OxA on the adenohypophysis level. The aim of this study was to evaluate the direct effect of orexin A on gonadotropin (LH and FSH) release from cultured pituitary cells of immature female rats as well as mature female rats (ovariectomized and ovariectomized and estradiol treated rats).
  
  
  METHODS
  The effect of 0.1 nM and 100 nM orexin A on LH and FSH release from anterior pituitary cells after 1 h of incubation was examined in immature female rats (IM) as well as mature female (ovariectomized - M/OVX; and ovariectomized and estradiol treated - M/OVX+E2) rats. The concentration of LH and FSH in medium was determined by RIA method.
  
  
  RESULTS
  Orexin A at a dose of 0.1 nM and 100 nM significantly stimulated LH secretion in IM group. In M/OVX group release of LH was inhibited by OxA only in higher dose (100 nM). No effect of orexin A on FSH secretion was found.
  
  
  CONCLUSIONS
  OxA may directly modulate LH secretion from cultured pituitary cells and it has the contradictory effect on LH release in immature and ovariectomized mature female rats.
authors.affiliation: Department of Clinical Neuroendocrinology, Medical Centre of Postgraduate Education, Warsaw, Poland. zne@cmkp.edu.pl
authors.forename: Lidia
authors.initials: L
authors.lastname: Martyńska
authors.forename: Ewa
authors.initials: E
authors.lastname: Wolińska-Witort
authors.forename: Magdalena
authors.initials: M
authors.lastname: Chmielowska
authors.forename: Wojciech
authors.initials: W
authors.lastname: Bik
authors.forename: Bogusława
authors.initials: B
authors.lastname: Baranowska
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D005640:Follicle Stimulating Hormone / Q000378:metabolism*; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000378:metabolism*; D009479:Neuropeptides / Q000378:metabolism*; D000068797:Orexins; D010052:Ovariectomy; D010902:Pituitary Gland / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D051381:Rats
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D009479:Neuropeptides; D000068797:Orexins; D004958:Estradiol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone