title: Alleviation of aluminum toxicity by hydrogen sulfide is related to elevated ATPase, and suppressed aluminum uptake and oxidative stress in barley.
abstract: Greenhouse hydroponic experiments were performed to evaluate potential role of H(2)S on Al toxicity in barley seedlings. Seedlings pretreated with 200 μM NaHS as a donor of H(2)S for 24h and subsequently exposed to 100 μM AlCl(3) for 24h had significantly longer roots than those without NaHS. The promoted root elongation was correlated with a substantial decrease in Al-induced overproduction of lipid peroxidation, electrolyte leakage and Al accumulation in roots, and a marked increase in Al-induced depress activities of Na(+)K(+)-ATPase and H(+)-ATPase. The alleviating role of H(2)S on Al-induced toxicity was also found in a time- and dose-dependent experiment. Addition of 200 and 400 μM NaHS to 100 μM AlCl(3) effectively alleviated Al-toxicity, markedly diminished Al-induced MDA accumulation, and increased chlorophyll content, net photosynthetic rate (Pn) and maximal photochemical efficiency (Fv/Fm) compared with Al alone. Exogenous H(2)S significantly elevated depressed CAT activities, and further improved root POD activity. Moreover, NaHS decreased Al accumulation, but elevated concentrations of S, P, Ca, Mg and Fe in plants. These data suggest that H(2)S-induced alleviation in Al toxicity is attributed to reduced Al uptake and MDA accumulation, improved uptake of P, Ca, Mg and Fe, and elevated ATPase and photosynthetic performance.
authors.affiliation: Department of Agronomy, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Dawood
authors.forename: Fangbin
authors.initials: F
authors.lastname: Cao
authors.forename: Muhammad Muzammil
authors.initials: MM
authors.lastname: Jahangir
authors.forename: Guoping
authors.initials: G
authors.lastname: Zhang
authors.forename: Feibo
authors.initials: F
authors.lastname: Wu
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000535:Aluminum / Q000493:pharmacokinetics / Q000633:toxicity*; D018533:Biomass; D002734:Chlorophyll / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D001467:Hordeum / Q000201:enzymology / Q000378:metabolism* / Q000502:physiology; D006862:Hydrogen Sulfide / Q000494:pharmacology*; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress; D010788:Photosynthesis
chemical_list: D002734:Chlorophyll; D008315:Malondialdehyde; D000535:Aluminum; D000251:Adenosine Triphosphatases; D006862:Hydrogen Sulfide