title: Anti-miR-155 oligonucleotide enhances chemosensitivity of U251 cell to taxol by inducing apoptosis.
abstract: The oncogene, microRNA-155, is significantly elevated in GBM (glioblastoma multiforme), regulating multiple genes associated with cancer cell proliferation, apoptosis and invasiveness. Thus, miR-155 can theoretically become a target for enhancement of the chemotherapy in cancer. Down-regulating miR-155 to enhance the effect of taxol has not been studied in human GBM. Human GBM U251 cells were treated with taxol and the miR-155 inhibitor alone or in combination. IC50 values were dramatically decreased in cells treated with miR-155 inhibitor combined with taxol, to a greater extent than those treated with taxol alone. Furthermore, the miR-155 inhibitor significantly enhanced apoptosis in U251 cells. The data suggest that miR-155 blockage increased the chemosensitivity to taxol in GBM cells, making combined treatment an effective therapeutic strategy for controlling the growth by inhibiting EAG1 expression.
authors.affiliation: Institute of Genetic Engineering, Southern Medical University, Guangzhou, Peoples Republic of China. Mengwei_126@126.com
authors.forename: Wei
authors.initials: W
authors.lastname: Meng
authors.forename: Ling
authors.initials: L
authors.lastname: Jiang
authors.forename: Lin
authors.initials: L
authors.lastname: Lu
authors.forename: Haiyan
authors.initials: H
authors.lastname: Hu
authors.forename: Hailang
authors.initials: H
authors.lastname: Yu
authors.forename: Dapeng
authors.initials: D
authors.lastname: Ding
authors.forename: Kun
authors.initials: K
authors.lastname: Xiao
authors.forename: Wenling
authors.initials: W
authors.lastname: Zheng
authors.forename: Hongbo
authors.initials: H
authors.lastname: Guo
authors.forename: Wenli
authors.initials: W
authors.lastname: Ma
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D015536:Down-Regulation / Q000187:drug effects; D051638:Ether-A-Go-Go Potassium Channels / Q000235:genetics / Q000378:metabolism; D005909:Glioblastoma / Q000378:metabolism / Q000473:pathology; D006801:Humans; D035683:MicroRNAs / Q000037:antagonists & inhibitors* / Q000378:metabolism; D016376:Oligonucleotides, Antisense / Q000378:metabolism*; D017239:Paclitaxel / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D051638:Ether-A-Go-Go Potassium Channels; C496884:KCNH1 protein, human; C524942:MIRN155 microRNA, human; D035683:MicroRNAs; D016376:Oligonucleotides, Antisense; D017239:Paclitaxel