title: Molecular cloning of a novel PPEF-1 gene variant from a T-cell lymphoblastic lymphoma cell line.
abstract: To determine if there is a gene variant of protein serine-threonine phosphatase with EF hand (PPEF-1) in T-cell lymphoblastic lymphoma SUP-T1 cell line, both in silico and in vitro approaches were conducted. In silico, a cDNA clone showing similar sequence to PPEF-1 was isolated from the SUP-T1 cDNA library and named PPEF-1V. The full-length of the PPEF-1V cDNA clone is a 2135bp containing a 1503bp open reading frame extending from 188bp to 1690bp, which corresponds to an encoded protein of 501 amino acid residues with a predicted molecular mass of 57.8 kDa. Alignment on both PPEF-1V and PPEF-1 sequences showed that PPEF-1V is a 350bp deletion in the nucleotide sequence of PPEF-1 from 128-477bp and a 152-amino-acid N-terminal deletion in the amino acid sequence of PPEF-1. In vitro, PPEF-1V transcript fragment was only highly expressed in T-cell lymphoblastic lymphoma cell line. In conclusion, the present patent showed that PPEF-1V could be a potential target for diagnosis or treatment of T-cell lymphoblastic lymphoma.
authors.affiliation: Division of General Surgery, Buddhist Tzu Chi General Hospital, Taipei Branch, Taiwan. president@visgeneer.com.tw
authors.forename: Ping
authors.initials: P
authors.lastname: Ho
authors.forename: Ken-Shwo
authors.initials: KS
authors.lastname: Dai
authors.forename: Hui-Ling
authors.initials: HL
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D016208:Databases, Factual; D017353:Gene Deletion; D006801:Humans; D015458:Leukemia, T-Cell / Q000175:diagnosis / Q000188:drug therapy / Q000473:pathology; D008969:Molecular Sequence Data; D009154:Mutation; D010330:Patents as Topic; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: C107424:PPEF1 protein, human; D010749:Phosphoprotein Phosphatases