title: Pro-apoptotic effects of Amblyomin-X in murine renal cell carcinoma "in vitro".
abstract: Renal cell carcinoma (RCC) is one of the most lethal urologic cancers and is highly resistant to both radiotherapy and chemotherapy. The recombinant protein Amblyomin-X, characterized as a Kunitz-type protease inhibitor, was obtained from a cDNA library from the salivary glands of the Amblyomma cajennense tick. This paper reports the biological effect of Amblyomin-X on inducing cell death by apoptotic process in vitro. For this purpose, the changes in morphological aspects of cells, the phosphatidylserine exposition and DNA degradation were evaluated after treatment with Amblyomin-X. We found that Amblyomin-X was able to induce apoptosis in Renca cells in a dose-dependent manner. So, the results presented here open perspectives for new researches and developing for Amblyomin-X in the treatment of RCC.
authors.affiliation: Laboratory of Biochemistry and Biophysics, Butantan Institute, Avenue Vital Brazil, 1500, CEP 05503-900, São Paulo, SP, Brazil.
authors.forename: Erica Mie
authors.initials: EM
authors.lastname: Akagi
authors.forename: Paulo Luiz de Sá
authors.initials: PL
authors.lastname: Júnior
authors.forename: Simone Michaela
authors.initials: SM
authors.lastname: Simons
authors.forename: Maria Helena
authors.initials: MH
authors.lastname: Bellini
authors.forename: Sandra Alves
authors.initials: SA
authors.lastname: Barreto
authors.forename: Ana Marisa
authors.initials: AM
authors.lastname: Chudzinski-Tavassi
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy / Q000473:pathology; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D060829:Arthropod Proteins; D002292:Carcinoma, Renal Cell / Q000188:drug therapy* / Q000473:pathology; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D007680:Kidney Neoplasms / Q000188:drug therapy* / Q000473:pathology; D051379:Mice; D012471:Salivary Proteins and Peptides / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: C559369:Amblyomin-X protein, Amblyomma cajennense; D000970:Antineoplastic Agents; D060829:Arthropod Proteins; D012471:Salivary Proteins and Peptides