title: Morphological transformation induced by multiwall carbon nanotubes on Balb/3T3 cell model as an in vitro end point of carcinogenic potential.
abstract: In this work we investigated the toxicological effects of nude and chemically functionalised (-NH(2), -OH and -COOH groups) multiwall carbon nanotubes (mwCNTs) using immortalised mouse fibroblasts cell line (Balb/3T3) as in vitro model, alternative to the use of animals, to assess basal cytotoxicity, carcinogenic potential, genotoxicity and cell interaction of nanomaterials (NM). Combining in vitro tests such as cell transformation assay and micronucleus with physicochemical and topological analysis, we obtained results showing no cytotoxicity and genotoxicity. Carcinogenic potential and mwCNTs interaction with cells were instead evident. We stressed the importance that different toxicological end points have to be considered when studying NM, therefore, assays able to detect long-term effects, such as carcinogenicity, must be taken into account together with a panel of tests able to detect more immediate effects like basal cytotoxicity or genotoxicity.
authors.affiliation: European Commission, Joint Research Centre, Institute for Health and Consumer Protection, Nanobiosciences Unit, Ispra, (VA), Italy. jessica.ponti@jrc.ec.europa.eu
authors.forename: Jessica
authors.initials: J
authors.lastname: Ponti
authors.forename: Francesca
authors.initials: F
authors.lastname: Broggi
authors.forename: Valentina
authors.initials: V
authors.lastname: Mariani
authors.forename: Laura
authors.initials: L
authors.lastname: De Marzi
authors.forename: Renato
authors.initials: R
authors.lastname: Colognato
authors.forename: Patrick
authors.initials: P
authors.lastname: Marmorato
authors.forename: Sabrina
authors.initials: S
authors.lastname: Gioria
authors.forename: Douglas
authors.initials: D
authors.lastname: Gilliland
authors.forename: César
authors.initials: C
authors.lastname: Pascual Garcìa
authors.forename: Stefania
authors.initials: S
authors.lastname: Meschini
authors.forename: Annarita
authors.initials: A
authors.lastname: Stringaro
authors.forename: Agnese
authors.initials: A
authors.lastname: Molinari
authors.forename: Hubert
authors.initials: H
authors.lastname: Rauscher
authors.forename: François
authors.initials: F
authors.lastname: Rossi
mesh_terms: D000826:Animal Testing Alternatives; D000818:Animals; D041702:BALB 3T3 Cells; D015197:Carcinogenicity Tests; D002273:Carcinogens / Q000633:toxicity*; D048430:Cell Shape / Q000187:drug effects*; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced* / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D051379:Mice; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced; D015162:Micronucleus Tests; D037742:Nanotubes, Carbon / Q000633:toxicity*; D018570:Risk Assessment; D013997:Time Factors
chemical_list: D002273:Carcinogens; D037742:Nanotubes, Carbon