title: Safety evaluation of an Ayurvedic medicine, Arogyavardhini vati on brain, liver and kidney in rats.
abstract: BACKGROUND
  Arogyavardhini vati, an Ayurvedic polyherbal formulation has been used for liver and skin disorders in the Ayurvedic system of medicine. However, toxicity due to the presence of heavy metals in this traditional medicine is a matter of concern.
  
  
  OBJECTIVE
  To evaluate the safety of Arogyavardhini vati on brain, liver and kidney in rats.
  
  
  METHODS
  Arogyavardhini vati at doses of 50, 250 and 500mg/kg (1, 5 and 10 times of human equivalent dose respectively), mercury chloride (1mg/kg) and normal saline were administered orally to male Wistar rats for 28 days. Behavioral parameters were assessed on day 1, 7th, 14th and 28th using Morris water maze, passive avoidance, elevated plus maze and rota rod. Biochemical parameters (acetyl-cholinesterase activity, malondialdehyde, reduced glutathione), histopathology and mercury level in brain, liver, kidney were assessed at the end of the experiment.
  
  
  RESULTS
  There was no significant change in behavioral parameters, acetyl-cholinesterase activity, liver function (ALT, AST, ALP and bilirubin) and kidney (serum urea and creatinine) function tests at all doses of Arogyavardhini vati (50, 250 and 500mg/kg) as compared to normal control. However, significant change was observed in mercury chloride treated group. Mercury chloride treated group as well as Arogyavardhini vati treated groups (50, 250 and 500mg/kg) showed increased levels of mercury in brain, liver and kidney as compared to normal control. Histopathological results showed significant cytoarchitectural changes in brain, liver and kidney architecture in mercury chloride treated group. Whereas, normal cytoarchitecture was observed at all doses of Arogyavardhini vati.
  
  
  CONCLUSIONS
  The finding of the present study suggests that Arogyavardhini vati in the doses equivalent up to 10 times of the human dose administered to rats for 28 days does not have appreciable toxicological effects on brain, liver and kidney.
authors.affiliation: Heavy Metal Toxicology Laboratory, Department of Pharmacology, All India Institute of Medical Sciences, New Delhi 110029, India.
authors.forename: Gajendra
authors.initials: G
authors.lastname: Kumar
authors.forename: Amita
authors.initials: A
authors.lastname: Srivastava
authors.forename: S K
authors.initials: SK
authors.lastname: Sharma
authors.forename: Y K
authors.initials: YK
authors.lastname: Gupta
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D001362:Avoidance Learning; D001522:Behavior, Animal / Q000187:drug effects; D001663:Bilirubin / Q000097:blood; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D003404:Creatinine / Q000097:blood; D004338:Drug Combinations; D006801:Humans; D007668:Kidney / Q000187:drug effects*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D018782:Maze Learning; D008515:Medicine, Ayurvedic; D008628:Mercury / Q000378:metabolism* / Q000494:pharmacology; D008670:Metals / Q000633:toxicity*; D008903:Minerals / Q000633:toxicity*; D010936:Plant Extracts / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D012965:Sodium Chloride / Q000494:pharmacology; D000637:Transaminases / Q000097:blood; D014508:Urea / Q000097:blood
chemical_list: D004338:Drug Combinations; D008670:Metals; D008903:Minerals; D010936:Plant Extracts; D012965:Sodium Chloride; C020973:arogya-wardhani; D014508:Urea; D003404:Creatinine; D000637:Transaminases; D000110:Acetylcholinesterase; D008628:Mercury; D001663:Bilirubin