title: [Co-metabolism of dual culture in the degradation of phenolic compounds].
abstract: The biodegradation of phenol, m-cresol and 4-chlorophenol by the pure and mixed culture of Pseudomonas putida and Candida tropicalis was investigated. The results showed that,with the initial concentrations of 100, 50 and 60 mg x L(-1) for phenol, m-cresol and 4-chlorophenol, respectively,and an increase in the absolute value of the kinetic constant k of 19.0%, 2.6% and 46.4% for the degradation of phenol, m-cresol and 4-chlorophenol, respectively, was reached by dual culture compared with pure culture. The activity assays of phenol hydroxylase and catechol 1, 2-dioxygenase demonstrated phenol was degraded via the ortho-cleavage pathway in both of the two species. Further experiments via high performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry techniques (LC-MS) suggested the degradation of three phenolic compounds could be accelerated due to co-metabolism by Pseudomonas putida of cis, cis-muconic acid, and 4-carboxymethylenebut-2-en-4-olide accumulated in phenol and 4-chlorophenol degradation, respectively, 3, 4-dihydroxybenzoic acid, 2-maleylacetate, and 3-oxoadipate in the degradation of m-cresol by Candida tropicalis in dual culture.
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China. sophia_heart@sina.com
authors.forename: Jiao
authors.initials: J
authors.lastname: Liu
authors.forename: Xiao-Qiang
authors.initials: XQ
authors.lastname: Jia
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Wen
mesh_terms: D001673:Biodegradation, Environmental; D041022:Candida tropicalis / Q000254:growth & development / Q000378:metabolism*; D002733:Chlorophenols / Q000302:isolation & purification / Q000378:metabolism*; D003408:Cresols / Q000302:isolation & purification / Q000378:metabolism*; D046508:Culture Techniques / Q000379:methods; D019800:Phenol / Q000302:isolation & purification / Q000378:metabolism*; D016958:Pseudomonas putida / Q000254:growth & development / Q000378:metabolism*; D014865:Waste Disposal, Fluid / Q000379:methods; D062065:Wastewater / Q000737:chemistry
chemical_list: D002733:Chlorophenols; D003408:Cresols; D019800:Phenol; D062065:Wastewater; C032538:4-cresol; C029107:4-chlorophenol