title: [Evaluation of four methods for detecting methicillin-resistant Staphylococcus aureus isolates from clinical specimens at a regional hospital in Mexico].
abstract: OBJECTIVE
  To estimate the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in clinical isolates and to compare different methods for detection of MRSA in a lab with limited available personnel and resources.
  
  
  METHODS
  140 Staphylococcus aureus strains isolated from patients in several departments were assayed for β-lactamase production, MIC-Vitek 2 oxacillin, ChromID MRSA, disk diffusion in agar for cefoxitin 30 μg and PBP2a detection. The results of conventional tests were compared with the "gold standard" PCR test for mecA gene. Cohen´s kappa index was also calculated in order to evaluate the intra assay agreement between the used methods.
  
  
  RESULTS
  The found prevalence was 90.7%. Sensitivity and specificity were: disk diffusion for cefoxitin 97 and 92% respectively, MIC Vitek 2-XL 97 and 69%, ChromoID MRSA 97 and 85%, and PBP2a detection 98 and 100%.
  
  
  CONCLUSIONS
  All methods are very good for detecting MRSA, choosing a method to use will depend on each laboratory infrastructure.
authors.affiliation: Laboratorio de Microbiología, Hospital General Regional No. 1, Instituto Mexicano del Seguro Social, México, DF, México. microbiol.inmuno@gmail.com
authors.forename: Gabriel
authors.initials: G
authors.lastname: Acosta-Pérez
authors.forename: Gabriela
authors.initials: G
authors.lastname: Rodríguez-Ábrego
authors.forename: Ernesto
authors.initials: E
authors.lastname: Longoria-Revilla
authors.forename: María Eugenia
authors.initials: ME
authors.lastname: Castro-Mussot
mesh_terms: D006761:Hospitals; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects / Q000302:isolation & purification*; D008800:Mexico; D008826:Microbial Sensitivity Tests