title: Mutation of a positively charged cytoplasmic motif within CD1d results in multiple defects in antigen presentation to NKT cells.
abstract: CD1d is an MHC class I-like molecule that presents glycolipid Ags to types I and II NKT cells. The YxxI motif in the cytoplasmic tail of CD1d contributes to its intracellular localization to the endolysosomal compartment and is important for Ag presentation to type I NKT cells. In this study, we identified the (327-329)RRR motif in CD1d and showed that it is critical for the control of CD1d intracellular trafficking and Ag presentation. The replacement of the arginines in this motif with alanines resulted in the extensive accumulation of CD1d in lysosomes but did not affect the cell surface expression. The defect in its cellular localization was accompanied by defects in Ag presentation to both type I and type II NKT cells. These results demonstrated that the (327-329)RRR motif of CD1d is required for proper cellular distribution of CD1d and optimal Ag presentation to both type I and type II NKT cells.
authors.affiliation: School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea.
authors.forename: Jung Hoon
authors.initials: JH
authors.lastname: Shin
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Park
authors.forename: Young Hyun
authors.initials: YH
authors.lastname: Shin
authors.forename: Hyunji
authors.initials: H
authors.lastname: Lee
authors.forename: Yoon-Kyung
authors.initials: YK
authors.lastname: Park
authors.forename: Sundo
authors.initials: S
authors.lastname: Jung
authors.forename: Se-Ho
authors.initials: SH
authors.lastname: Park
mesh_terms: D020816:Amino Acid Motifs / Q000235:genetics; D000818:Animals; D017951:Antigen Presentation / Q000235:genetics* / Q000276:immunology*; D055610:Antigens, CD1d / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D001120:Arginine / Q000235:genetics; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000201:enzymology / Q000235:genetics / Q000276:immunology; D003593:Cytoplasm / Q000201:enzymology / Q000235:genetics* / Q000276:immunology*; D007424:Intracellular Fluid / Q000201:enzymology / Q000276:immunology; D008247:Lysosomes / Q000201:enzymology / Q000235:genetics / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D016297:Mutagenesis, Site-Directed; D055611:Natural Killer T-Cells / Q000145:classification / Q000276:immunology* / Q000473:pathology; D021381:Protein Transport / Q000235:genetics / Q000276:immunology; D017384:Sequence Deletion / Q000235:genetics / Q000276:immunology; D055672:Static Electricity
chemical_list: D055610:Antigens, CD1d; C526879:Cd1d1 protein, mouse; D001120:Arginine