title: Effects of ethanol on CYP2E1 levels and related oxidative stress using a standard balanced diet.
abstract: Expression of cytochrome P4502E1 (CYP2E1) is very much influenced by nutritional factors, especially carbohydrate consumption, and various results concerning the expression of CYP2E1 were obtained with a low-carbohydrate diet. This study describes the effects of ethanol treatment on CYP2E1 levels and its relationship with oxidative stress using a balanced standard diet to avoid low or high carbohydrate consumption. Rats were fed for 1, 2, 3, or 4 weeks a commercial diet plus an ethanol-sucrose solution. The results have shown that ethanol administration was associated with CYP2E1 induction and stabilization without related oxidative stress. Our findings suggest that experimental models with a low-carbohydrate/high-fat diet produce some undesirable CYP2E1 changes that are not present when a balanced standard diet is given.
authors.affiliation: Universidade Federal de São Paulo, Diadema, São Paulo, Brasil. lazzalis@uol.com.br
authors.forename: Ligia A
authors.initials: LA
authors.lastname: Azzalis
authors.forename: Fernando L A
authors.initials: FL
authors.lastname: Fonseca
authors.forename: Karin A
authors.initials: KA
authors.lastname: Simon
authors.forename: Fernanda
authors.initials: F
authors.lastname: Schindler
authors.forename: Leandro
authors.initials: L
authors.lastname: Giavarotti
authors.forename: Hugo P
authors.initials: HP
authors.lastname: Monteiro
authors.forename: Luis A
authors.initials: LA
authors.lastname: Videla
authors.forename: Virgínia B C
authors.initials: VB
authors.lastname: Junqueira
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000704:Analysis of Variance; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D019392:Cytochrome P-450 CYP2E1 / Q000378:metabolism*; D004032:Diet; D004790:Enzyme Induction / Q000187:drug effects*; D000431:Ethanol / Q000008:administration & dosage / Q000633:toxicity*; D005978:Glutathione / Q000378:metabolism; D008099:Liver / Q000187:drug effects*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D018709:Statistics, Nonparametric
chemical_list: D017382:Reactive Oxygen Species; D000431:Ethanol; D019392:Cytochrome P-450 CYP2E1; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D005978:Glutathione