title: Adsorption and disruption of lipid bilayers by nanoscale protein aggregates.
abstract: Nanoparticles taken into biological systems can have biological impacts through their interactions with cell membranes, accompanied by protein adsorption onto the nanoparticle surfaces, forming a so-called protein corona. Our current research aims to demonstrate that nanoscale protein aggregates behave like such nanoparticles with regard to the interaction with lipid membranes. In this study, the adsorption and disruption of the lipid membranes by protein aggregates were investigated using amyloid fibrils and nanoscale thermal aggregates of lysozyme. Both types of protein aggregates had disruptive effects on the negatively charged liposomes, similar to polycationic nanoparticles. Interestingly, adsorption of liposomes on the amyloid fibrils preceding disruption occurred even if the net charge of the liposome was zero, suggesting the importance of hydrophobic interactions in addition to electrostatic interactions. The results of the present study provide new insights into the biological impacts of nanoparticles in vivo.
authors.affiliation: Nanosystem Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305-8562, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Hirano
authors.forename: Hiroki
authors.initials: H
authors.lastname: Yoshikawa
authors.forename: Shuhei
authors.initials: S
authors.lastname: Matsushita
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Yamada
authors.forename: Kentaro
authors.initials: K
authors.lastname: Shiraki
mesh_terms: D000327:Adsorption; D000682:Amyloid / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology; D011506:Proteins / Q000737:chemistry*
chemical_list: D000682:Amyloid; D008051:Lipid Bilayers; D008081:Liposomes; D011506:Proteins