title: Semiautomated device for batch extraction of metabolites from tissue samples.
abstract: Metabolomics has become a mainstream analytical strategy for investigating metabolism. The quality of data derived from these studies is proportional to the consistency of the sample preparation. Although considerable research has been devoted to finding optimal extraction protocols, most of the established methods require extensive sample handling. Manual sample preparation can be highly effective in the hands of skilled technicians, but an automated tool for purifying metabolites from complex biological tissues would be of obvious utility to the field. Here, we introduce the semiautomated metabolite batch extraction device (SAMBED), a new tool designed to simplify metabolomics sample preparation. We discuss SAMBED's design and show that SAMBED-based extractions are of comparable quality to extracts produced through traditional methods (13% mean coefficient of variation from SAMBED versus 16% from manual extractions). Moreover, we show that aqueous SAMBED-based methods can be completed in less than a quarter of the time required for manual extractions.
authors.affiliation: National Magnetic Resonance Facility at Madison, Department of Biochemistry, University of Wisconsin-Madison, 433 Babcock Drive, Madison, Wisconsin 53706-1544, USA.
authors.forename: James J
authors.initials: JJ
authors.lastname: Ellinger
authors.forename: Dan C
authors.initials: DC
authors.lastname: Miller
authors.forename: Ian A
authors.initials: IA
authors.lastname: Lewis
authors.forename: John L
authors.initials: JL
authors.lastname: Markley
mesh_terms: D000818:Animals; D001331:Automation; D002417:Cattle; D008099:Liver / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D055432:Metabolomics / Q000295:instrumentation* / Q000379:methods*; D013048:Specimen Handling / Q000295:instrumentation*