title: Binding of PF2 lectin from Olneya tesota to gut proteins of Zabrotes subfasciatus larvae associated with the insecticidal mechanism.
abstract: Zabrotes subfasciatus  (Boheman) is the main pest of common beans ( Phaselous vulgaris ). Wild legume seeds from  Olneya tesota  contain a lectin, PF2, that shows insecticidal activities against this insect. The binding of PF2 to midgut glycoproteins of 20-day-old larvae was evaluated using PF2 affinity chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the proteins retained on the gel revealed several putative glycoproteins, ranging in mass from 17 to 97 kDa. Subsequent protein digestion and analysis by liquid chromatography-tandem mass spectrometry (LC-MS/MS) provided amino acid fragments that identified an α-tubulin, cytochrome c oxidase subunit I, an odorant receptor, and a lysozyme from available insect sequence databases. The potential of these proteins to serve as part of the mechanisms involved in the insecticidal activity of PF2 to  Z. subfasciatus  is discussed.
authors.affiliation: Centro de Investigación en Alimentación y Desarrollo, Hermosillo, Sonora, Mexico.
authors.forename: Irlanda
authors.initials: I
authors.lastname: Lagarda-Diaz
authors.forename: M Refugio
authors.initials: MR
authors.lastname: Robles-Burgeño
authors.forename: Ana M
authors.initials: AM
authors.lastname: Guzman-Partida
authors.forename: Dawn
authors.initials: D
authors.lastname: Geiser
authors.forename: Joy
authors.initials: J
authors.lastname: Winzerling
authors.forename: Luz
authors.initials: L
authors.lastname: Vazquez-Moreno
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001517:Coleoptera / Q000378:metabolism*; D007887:Fabaceae / Q000737:chemistry*; D041981:Gastrointestinal Tract / Q000737:chemistry / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism; D019476:Insect Proteins / Q000737:chemistry / Q000378:metabolism*; D007306:Insecticides / Q000378:metabolism*; D007814:Larva / Q000378:metabolism; D037102:Lectins / Q000378:metabolism*; D008969:Molecular Sequence Data; D012639:Seeds / Q000737:chemistry*
chemical_list: D006023:Glycoproteins; D019476:Insect Proteins; D007306:Insecticides; D037102:Lectins