title: Streptococcus suis II immunoassay based on thorny gold nanoparticles and surface enhanced Raman scattering.
abstract: An immunoassay based on surface enhanced Raman scattering (SERS) spectroscopy was developed to detect muramidase released protein (MRP) antibody against Streptococcus suis II (SS2) utilizing thorny gold nanoparticles (tAuNPs) as SERS substrates. Initially, tAuNPs with multi-branches were prepared by the seed-mediated growth method in the absence of templates and surfactants, facilitating p-mercaptobenzoic acid (pMBA) conjugation covalently onto the tAuNPs through S-Au bonds. The obtained immuno-SERS tag affording strong Raman signals made it possible to establish an application of indirect detection of the MRP antibody against SS2 with a sandwich assay at a highly sensitive level. The Raman intensity at 1588 cm(-1) was proportional to the logarithm of the concentration of MRP antibody in the range of 10 pg mL(-1) to 0.1 μg mL(-1). The detection sensitivity was significantly improved to 0.1 pg mL(-1) by using the immuno-SERS tags. Furthermore, the proposed SERS approach was applied to detect MRP antibody in pig serum samples, and the results agreed well with those of ELISA, indicating great potential for clinical application in diagnostic immunoassays.
authors.affiliation: College of Science, State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Chen
authors.forename: Heyou
authors.initials: H
authors.lastname: Han
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Luo
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology; D006046:Gold / Q000737:chemistry*; D007118:Immunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000032:analysis / Q000276:immunology; D053768:Metal Nanoparticles / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D017011:Streptococcus suis; D013499:Surface Properties; D013552:Swine
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D007074:Immunoglobulin G; C421489:MRP protein, Streptococcus suis; D006046:Gold