title: The iron-regulated surface proteins IsdA, IsdB, and IsdH are not required for heme iron utilization in Staphylococcus aureus.
abstract: The iron-regulated surface determinant proteins (Isd) of Staphylococcus aureus are expressed during iron limitation and have been proposed to be involved in the scavenging of iron from heme. In this study, the genes encoding the surface proteins IsdA, IsdB, and IsdH were inactivated in order to determine their combined role. The triple mutant was found to have no defect in growth under any conditions of iron limitation tested. Also using a mouse septic arthritis model of S. aureus systemic disease, no significant difference in bacterial load was observed for the triple mutant, compared with its otherwise isogenic parent.
authors.affiliation: Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, UK.
authors.forename: Alexander F
authors.initials: AF
authors.lastname: Hurd
authors.forename: Jorge
authors.initials: J
authors.lastname: Garcia-Lara
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Rauter
authors.forename: Michaël
authors.initials: M
authors.lastname: Cartron
authors.forename: Ramlan
authors.initials: R
authors.lastname: Mohamed
authors.forename: Simon J
authors.initials: SJ
authors.lastname: Foster
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000378:metabolism*; D001170:Arthritis, Infectious / Q000382:microbiology / Q000473:pathology; D058491:Bacterial Load; D027682:Cation Transport Proteins / Q000235:genetics / Q000378:metabolism*; D004195:Disease Models, Animal; D015964:Gene Expression Regulation, Bacterial; D055786:Gene Knockout Techniques; D006418:Heme / Q000378:metabolism*; D007501:Iron / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D013203:Staphylococcal Infections / Q000382:microbiology / Q000473:pathology; D013211:Staphylococcus aureus / Q000254:growth & development / Q000378:metabolism*
chemical_list: D000942:Antigens, Bacterial; D027682:Cation Transport Proteins; C512791:IsdA protein, Staphylococcus aureus; C510010:IsdB protein, Staphylococcus aureus; C512792:IsdH protein, Staphylococcus aureus; D008565:Membrane Proteins; D011956:Receptors, Cell Surface; D006418:Heme; D007501:Iron