title: Analysis of hepatitis C virus intrahost diversity across the coding region by ultradeep pyrosequencing.
abstract: Hepatitis C virus (HCV) is the leading cause of liver disease worldwide. In this study, we analyzed four treatment-naïve patients infected with subtype 1a and performed Roche/454 pyrosequencing across the coding region. We report the presence of low-level drug resistance mutations that would most likely have been missed using conventional sequencing methods. The approach described here is broadly applicable to studies of viral diversity and could help to improve the efficacy of direct-acting antiviral agents (DAA) in the treatment of HCV-infected patients.
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, Wisconsin, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Lauck
authors.forename: Mónica V
authors.initials: MV
authors.lastname: Alvarado-Mora
authors.forename: Ericka A
authors.initials: EA
authors.lastname: Becker
authors.forename: Dipankar
authors.initials: D
authors.lastname: Bhattacharya
authors.forename: Rob
authors.initials: R
authors.lastname: Striker
authors.forename: Austin L
authors.initials: AL
authors.lastname: Hughes
authors.forename: Flair J
authors.initials: FJ
authors.lastname: Carrilho
authors.forename: David H
authors.initials: DH
authors.lastname: O'Connor
authors.forename: João R Rebello
authors.initials: JR
authors.lastname: Pinho
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology; D024882:Drug Resistance, Viral; D016174:Hepacivirus / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D006526:Hepatitis C / Q000821:virology*; D058507:Host Specificity; D006801:Humans; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D000998:Antiviral Agents