title: Single-prolonged stress induce changes of CaM/CaMKIIα in the rats of dorsal raphe nucleus.
abstract: Ca2+/calmodulin-dependent protein kinase IIα (CaMKIIα) is identified as a Ca2+-dependent kinase in brain involved in the activation of Tryptophan hydroxylase (TPH) acting through direct phosphorylation of TPH, and playing key roles in the signaling pathways initiated by various G protein-coupled 5-HT receptors. The goal of this study is to detect whether there are changes of CaM and CaMKIIα in dorsal raphe nucleus in the rats exposed to single-prolonged stress (SPS), which is a model employed in post-traumatic stress disorder (PTSD) study extensively. A total of 90 male Wistar rats were randomly divided into a normal control group and SPS groups of 7d, 14d. The changes of CaM/CaMKIIα were detected by immunohistochemistry, reverse transcription-polymerase chain reaction and western blot. Our results demonstrate that both expressions of CaM and CaMKIIα significantly increase (P < 0.001) in the SPS 7d group than that in the control group, and then decreased dramatically (P < 0.001) 14 days after SPS. Our results confirm that SPS induce changes of CaM/CaMKIIα in the dorsal raphe nucleus. Changes of CaM/CaMKIIα may be associated with the activation of 5-HT1A receptor, and may contribute to the progress of molecular mechanism of PTSD.
authors.affiliation: Department of Histology and Embryology, Institute of Pathology and Pathophysiology, Basic Medical Sciences College, China Medical University, 92 North 2nd Road, Shenyang 110001, Liaoning Province, People’s Republic of China.
authors.forename: Huaju
authors.initials: H
authors.lastname: Xie
authors.forename: Fang
authors.initials: F
authors.lastname: Han
authors.forename: Xiuyu
authors.initials: X
authors.lastname: Shi
mesh_terms: D000818:Animals; D015153:Blotting, Western; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000235:genetics / Q000378:metabolism*; D002147:Calmodulin / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D011903:Raphe Nuclei / Q000201:enzymology*; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013312:Stress, Physiological
chemical_list: D002147:Calmodulin; D012333:RNA, Messenger; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2