title: Adhesion and differentiation of adipose-derived stem cells on a substrate with immobilized fibroblast growth factor.
abstract: Control of cell-matrix interactions plays a role in the regulation of stem cell function. In this study basic fibroblast growth factor (bFGF) linked to maltose-binding protein (MBP) was designed as a matrix for cell adhesion. MBP-FGF was immobilized on polystyrene (PS) surfaces by spontaneous adsorption. The amount of MBP-bFGF immobilized on the PS surface increased with increasing protein concentration, being 158 ng cm(-2) at 10 μg ml(-1) protein. Human adipose-derived stem cell (hASC) adhesion to MBP-bFGF immobilized on a PS surface (PS-MBP-bFGF) was inhibited by heparin. Integrin signaling and cell spreading of hASC on PS-MBP-bFGF were down-regulated compared with those on fibronectin-coated surfaces or tissue culture polystyrene (TCP). hASC differentiated into adipocytes, which stained positive for lipid vacuoles with Oil Red, more readily on PS-MBP-bFGF than on TCP. In contrast, hASC hardly differentiated into osteoblast on PS-MBP-bFGF or on TCP. These results suggest that the mechanism of hASC adhesion to MBP-bFGF immobilized on a PS substrate is mediated by a specific interaction between bFGF and heparin, and that the adhesion mechanism might provide an insight into the design of biomaterials to control the fate of stem cells.
authors.affiliation: Center for Biomaterials, Korea Institute of Science and Technology, Seoul, Republic of Korea.
authors.forename: Jung Mi
authors.initials: JM
authors.lastname: Kang
authors.forename: Min
authors.initials: M
authors.lastname: Han
authors.forename: In Su
authors.initials: IS
authors.lastname: Park
authors.forename: Youngmee
authors.initials: Y
authors.lastname: Jung
authors.forename: Soo Hyun
authors.initials: SH
authors.lastname: Kim
authors.forename: Sang-Heon
authors.initials: SH
authors.lastname: Kim
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry* / Q000494:pharmacology*; D006801:Humans; D008422:Materials Testing; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D020099:Coated Materials, Biocompatible; D016222:Fibroblast Growth Factor 2