title: Early detection of insulin fibrillation: a fluorescence lifetime assay to probe the pre-fibrillar regime.
abstract: Using human insulin, we report for the first time, the evaluation of fluorescence lifetime of an extrinsically added fluorogenic dye, Thioflavin T (ThT), as a more sensitive and convenient method for the assessment of fibrillation in the pre-fibrillar regime.
authors.affiliation: Radiation & Photochemistry Division, Bhabha Atomic Research Centre, Mumbai 400 085, India. jyotim@barc.gov.in
authors.forename: Jyotirmayee
authors.initials: J
authors.lastname: Mohanty
authors.forename: Sharmistha Dutta
authors.initials: SD
authors.lastname: Choudhury
authors.forename: Haridas
authors.initials: H
authors.lastname: Pal
authors.forename: Achikanath C
authors.initials: AC
authors.lastname: Bhasikuttan
mesh_terms: D052160:Benzothiazoles; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D007328:Insulin / Q000737:chemistry*; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D012996:Solutions; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013844:Thiazoles / Q000737:chemistry; D013997:Time Factors
chemical_list: D052160:Benzothiazoles; D005456:Fluorescent Dyes; D007328:Insulin; D012996:Solutions; D013844:Thiazoles; C009462:thioflavin T