title: Characterization and immunomodulatory function comparison of various bursal-derived peptides isolated from the humoral central immune organ.
abstract: The bursa of Fabricius (BF) is the acknowledged central immune organ, which is important to the B cell differentiation and antibody production. However, due to difficult purification, the immunomodulatory peptides from BF were little reported. In this study, the extract samples of BF were taken to a chromatographic analysis by RP-HPLC. Five novel low molecular weight peptides were isolated from BF, with amino acid sequences of YEYAY, RMYEE, GPPAT, AGCCNG, and RRL, and named as Bursal pentapeptide (BPP)-III, -IV, -V, and Bursal hexapeptide (BHP), and Bursal tripeptide (BTP), respectively. BSP-I, BSP-II, BPP-I and BPP-II are recently reported to be the bursal-derived bioactive peptides. In this paper, we analyzed the chemical formula and characteristics of these nine bursal-derived peptides. The immunization comparative experiment verified the different immunomodulatory activity of these nine bursal peptides on antibody and cytokine productions. Furthermore, the results showed that at reachable concentrations, BPP-II and BPP-I induced antibody productions, lymphocyte viabilities and cytokine responses in different dose-dependent manner in the immunized mice model, respectively. These results provided important orientations for the comprehensively understanding and study of the humoral central immune system of human, and provided a novel insight on the treatment of serious disease and immune improvement of human.
authors.affiliation: Division of Key Lab of Animal Disease Diagnosis and Immunology of China's Department of Agriculture, Nanjing Agriculture University, Nanjing 210095, China.
authors.forename: Xiu-Li
authors.initials: XL
authors.lastname: Feng
authors.forename: Qing-Tao
authors.initials: QT
authors.lastname: Liu
authors.forename: Rui-Bing
authors.initials: RB
authors.lastname: Cao
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.forename: Yuan-Peng
authors.initials: YP
authors.lastname: Zhang
authors.forename: Ke
authors.initials: K
authors.lastname: Liu
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Liu
authors.forename: Jian-Chao
authors.initials: JC
authors.lastname: Wei
authors.forename: Xin-Feng
authors.initials: XF
authors.lastname: Li
authors.forename: Pu-Yan
authors.initials: PY
authors.lastname: Chen
mesh_terms: D000276:Adjuvants, Immunologic / Q000302:isolation & purification / Q000494:pharmacology*; D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation; D000956:Antigens, Viral / Q000276:immunology; D030161:Avian Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D002060:Bursa of Fabricius / Q000737:chemistry*; D002645:Chickens; D005260:Female; D006801:Humans; D056724:Immunity, Humoral / Q000187:drug effects; D009980:Influenza A virus / Q000276:immunology; D007371:Interferon-gamma / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D051379:Mice; D008970:Molecular Weight; D009842:Oligopeptides / Q000302:isolation & purification / Q000494:pharmacology*; D014611:Vaccination
chemical_list: D000276:Adjuvants, Immunologic; D000956:Antigens, Viral; D030161:Avian Proteins; D009842:Oligopeptides; D015847:Interleukin-4; D007371:Interferon-gamma