title: The effect of raw soybean on oxidative status of digestive organs in mice.
abstract: The present study was undertaken to specify the effect of raw soybean on oxidative status of digestive organs in mice. For this purpose, thirty male (C57BL/6J) mice were randomly divided into three groups and fed on different diets as follows: Group 1 was fed on control diet, Group 2 was fed on raw soybean diet and Group 3 was fed on raw soybean diet supplemented with 30 mg/kg cysteamine. After two weeks of feeding, duodenum, liver and pancreas samples were collected to measure oxidative and antioxidative parameters. The results show that ingestion of raw soybean markedly increased contents of superoxide anion and malondialdehyde (MDA) and activity of inducible nitric oxide synthase (iNOS), decreased activity of superoxide dismutase (SOD), T-AOC and content of reduced glutathione (GSH) in digestive organs of mice (P < 0.05). In the group fed with raw soybean diet supplemented with cysteamine, oxidative stress was mitigated. However, oxidative parameter levels were still higher than those of control diet-fed group. The present study indicates that ingestion of raw soybean could result in an imbalance between oxidant and antioxidant, and thus induce oxidative stress in digestive organs of mice.
authors.affiliation: Institute of Food Science and Engineering, Jilin Agricultural University, Changchun, Jilin 130118, China. jjnong2008@126.com
authors.forename: Chunmei
authors.initials: C
authors.lastname: Gu
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Qu
authors.forename: Lingling
authors.initials: L
authors.lastname: Han
authors.forename: Xinxiu
authors.initials: X
authors.lastname: Song
authors.forename: Linlin
authors.initials: L
authors.lastname: Zhao
authors.forename: Wenfa
authors.initials: W
authors.lastname: Lu
mesh_terms: D000818:Animals; D004386:Duodenum / Q000187:drug effects / Q000378:metabolism*; D004435:Eating; D005978:Glutathione / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D018384:Oxidative Stress; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism*; D030262:Soybean Proteins / Q000494:pharmacology*; D013025:Glycine max / Q000737:chemistry; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism
chemical_list: D030262:Soybean Proteins; D013481:Superoxides; D008315:Malondialdehyde; D052247:Nitric Oxide Synthase Type II; D013482:Superoxide Dismutase; D005978:Glutathione