title: Correlation of PDCD5 and apoptosis in hair cells and spiral ganglion neurons of different age of C57BL/6J mice.
abstract: This study examined the expression pattern of programmed cell death 5 (PDCD5) in cochlear hair cells and spiral ganglion neurons (SGNs) and its association with age-related hearing loss in mice. Sixty C57BL/6J (C57) mice at different ages were divided into four groups (3, 6, 9 or 12 months). PDCD5 expression was detected by using immunohistochemistry, real-time PCR and Western blot. Morphological change of the cochleae was also evaluated by using immunoassay. The results showed that the expression of PDCD5 had a gradual increase with ageing in both protein and RNA levels in C57 mice, as well as gradually increased apoptosis of cochlear hair cells and SGNs. In addition, we also found that caspase-3 activity was enhanced and its expression was enhanced with ageing. It is implied that overexpression of PDCD5 causes the increase in caspase-3 activity and the subsequent increase of apoptosis in cochlear hair cells and SGNs, and thereby plays a role in the pathogenesis of presbycusis. Thus, PDCD5 may be a new target site for the treatment and prevention of age-related hearing loss.
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Huazhong University of Science and Technology, Wuhan, 430032, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Huazhong University of Science and Technology, Wuhan, 430032, China. qi7chu@163.com.
authors.forename: Hanqi
authors.initials: H
authors.lastname: Chu
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Huazhong University of Science and Technology, Wuhan, 430032, China.
authors.forename: Liangqiang
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Pediatric Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430032, China.
authors.forename: Heyun
authors.initials: H
authors.lastname: Gao
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Huazhong University of Science and Technology, Wuhan, 430032, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Xiong
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Huazhong University of Science and Technology, Wuhan, 430032, China.
authors.forename: Qingguo
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Huazhong University of Science and Technology, Wuhan, 430032, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Huazhong University of Science and Technology, Wuhan, 430032, China.
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Huang
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Huazhong University of Science and Technology, Wuhan, 430032, China.
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Cui
mesh_terms: D000375:Aging / Q000473:pathology / Q000502:physiology*; D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism*; D002478:Cells, Cultured; D005260:Female; D006198:Hair Cells, Auditory / Q000166:cytology / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009363:Neoplasm Proteins / Q000378:metabolism*; D013136:Spiral Ganglion / Q000166:cytology / Q000502:physiology*; D013223:Statistics as Topic
chemical_list: D051017:Apoptosis Regulatory Proteins; D009363:Neoplasm Proteins; C486488:Pdcd5 protein, mouse