title: The accessibility in the external part of the TM5 of the glutamate transporter EAAT1 is conformationally sensitive during the transport cycle.
abstract: BACKGROUND
  Excitatory amino acid transporter 1 (EAAT1) is a glutamate transporter which is a key element in the termination of the synaptic actions of glutamate. It serves to keep the extracellular glutamate concentration below neurotoxic level. However the functional significance and the change of accessibility of residues in transmembrane domain (TM) 5 of the EAAT1 are not clear yet.
  
  
  RESULTS
  We used cysteine mutagenesis with treatments with membrane-impermeable sulfhydryl reagent MTSET [(2-trimethylammonium) methanethiosulfonate] to investigate the change of accessibility of TM5. Cysteine mutants were introduced from position 291 to 300 of the cysteine-less version of EAAT1. We checked the activity and kinetic parameters of the mutants before and after treatments with MTSET, furthermore we analyzed the effect of the substrate and blocker on the inhibition of the cysteine mutants by MTSET. Inhibition of transport by MTSET was observed in the mutants L296C, I297C and G299C, while the activity of K300C got higher after exposure to MTSET. V(max) of L296C and G299C got lower while that of K300C got higher after treated by MTSET. The L296C, G299C, K300C single cysteine mutants showed a conformationally sensitive reactivity pattern. The sensitivity of L296C to MTSET was potentiated by glutamate and TBOA,but the sensitivity of G299C to MTSET was potentiated only by TBOA.
  
  
  CONCLUSIONS
  All these facts suggest that the accessibility of some positions of the external part of the TM5 is conformationally sensitive during the transport cycle. Our results indicate that some residues of TM5 take part in the transport pathway during the transport cycle.
authors.affiliation: China-America Cancer Research Institute, Guangdong Medical College, Dongguan, Guangdong, China.
authors.forename: Xiuping
authors.initials: X
authors.lastname: Zhang
authors.forename: Shaogang
authors.initials: S
authors.lastname: Qu
mesh_terms: D001224:Aspartic Acid / Q000378:metabolism / Q000493:pharmacokinetics; D001665:Binding Sites / Q000235:genetics; D001692:Biological Transport / Q000235:genetics / Q000502:physiology; D027341:Excitatory Amino Acid Transporter 1 / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D018698:Glutamic Acid / Q000378:metabolism* / Q000493:pharmacokinetics; D006367:HeLa Cells; D006801:Humans; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D011485:Protein Binding / Q000235:genetics; D017510:Protein Folding; D054730:Protein Interaction Domains and Motifs / Q000235:genetics / Q000502:physiology*; D017433:Protein Structure, Secondary; D021381:Protein Transport / Q000235:genetics / Q000502:physiology; D014316:Tritium / Q000493:pharmacokinetics
chemical_list: D027341:Excitatory Amino Acid Transporter 1; D014316:Tritium; D001224:Aspartic Acid; D018698:Glutamic Acid