title: Response of adipose tissue to early infection with Trypanosoma cruzi (Brazil strain).
abstract: Brown adipose tissue (BAT) and white adipose tissue (WAT) and adipocytes are targets of Trypanosoma cruzi infection. Adipose tissue obtained from CD-1 mice 15 days after infection, an early stage of infection revealed a high parasite load. There was a significant increase in macrophages in infected adipose tissue and a reduction in lipid accumulation, adipocyte size, and fat mass and increased expression of lipolytic enzymes. Infection increased levels of Toll-like receptor (TLR) 4 and TLR9 and in the expression of components of the mitogen-activated protein kinase pathway. Protein and messenger RNA (mRNA) levels of peroxisome proliferator-activated receptor γ were increased in WAT, whereas protein and mRNA levels of adiponectin were significantly reduced in BAT and WAT. The mRNA levels of cytokines, chemokines, and their receptors were increased. Nuclear Factor Kappa B levels were increased in BAT, whereas Iκκ-γ levels increased in WAT. Adipose tissue is an early target of T. cruzi infection.
authors.affiliation: Department of Pathology, Diabetes Research and Training Center, Albert Einstein College of Medicine, Bronx, NY, USA. fnu.nagajyothi@einstein.yu.edu
authors.forename: Fnu
authors.initials: F
authors.lastname: Nagajyothi
authors.forename: Mahalia S
authors.initials: MS
authors.lastname: Desruisseaux
authors.forename: Fabiana S
authors.initials: FS
authors.lastname: Machado
authors.forename: Rajendra
authors.initials: R
authors.lastname: Upadhya
authors.forename: Dazhi
authors.initials: D
authors.lastname: Zhao
authors.forename: Gary J
authors.initials: GJ
authors.lastname: Schwartz
authors.forename: Mauro M
authors.initials: MM
authors.lastname: Teixeira
authors.forename: Chris
authors.initials: C
authors.lastname: Albanese
authors.forename: Michael P
authors.initials: MP
authors.lastname: Lisanti
authors.forename: Streamson C
authors.initials: SC
authors.lastname: Chua
authors.forename: Louis M
authors.initials: LM
authors.lastname: Weiss
authors.forename: Philipp E
authors.initials: PE
authors.lastname: Scherer
authors.forename: Herbert B
authors.initials: HB
authors.lastname: Tanowitz
mesh_terms: D017667:Adipocytes / Q000378:metabolism* / Q000469:parasitology / Q000473:pathology; D052242:Adiponectin / Q000378:metabolism; D002001:Adipose Tissue, Brown / Q000378:metabolism* / Q000469:parasitology / Q000473:pathology; D052436:Adipose Tissue, White / Q000378:metabolism* / Q000469:parasitology / Q000473:pathology; D000818:Animals; D014355:Chagas Disease / Q000469:parasitology* / Q000473:pathology; D018925:Chemokines / Q000378:metabolism; D016207:Cytokines; D051550:I-kappa B Kinase / Q000378:metabolism; D008297:Male; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D047495:PPAR gamma / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D019707:Receptors, Chemokine / Q000378:metabolism; D015398:Signal Transduction; D051197:Toll-Like Receptor 4 / Q000378:metabolism; D051217:Toll-Like Receptor 9 / Q000378:metabolism; D014349:Trypanosoma cruzi
chemical_list: D052242:Adiponectin; D018925:Chemokines; D016207:Cytokines; D016328:NF-kappa B; D047495:PPAR gamma; D012333:RNA, Messenger; D019707:Receptors, Chemokine; C493487:Tlr4 protein, mouse; C495370:Tlr9 protein, mouse; D051197:Toll-Like Receptor 4; D051217:Toll-Like Receptor 9; D051550:I-kappa B Kinase; D020928:Mitogen-Activated Protein Kinases