title: An energy-saving glutathione production method from low-temperature cooked rice using amylase-expressing Saccharomyces cerevisiae.
abstract: Glutathione is a valuable tripeptide that is widely used in the pharmaceutical, food, and cosmetic industries. Glutathione is industrially produced by fermentation using Saccharomyces cerevisiae. Before the glutathione fermentation process with S. cerevisiae, a glucose extraction process from starchy materials is required. This glucose extraction is usually carried out by converting starchy materials to starch using high-temperature cooking and subsequent hydrolysis by amylases to convert starch to glucose. In this study, to develop an energy-saving glutathione production process by reducing energy consumption during the cooking step, we efficiently produced glutathione from low-temperature cooked rice using amylase-expressing S. cerevisiae. The combination of the amylase-expressing yeast with low-temperature cooking is potentially applicable to a variety of energy-saving bio-production methods of chemicals from starchy bio-resources.
authors.affiliation: Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Japan.
authors.forename: Kiyotaka Y
authors.initials: KY
authors.lastname: Hara
authors.forename: Songhee
authors.initials: S
authors.lastname: Kim
authors.forename: Kentaro
authors.initials: K
authors.lastname: Kiriyama
authors.forename: Hideyo
authors.initials: H
authors.lastname: Yoshida
authors.forename: Shogo
authors.initials: S
authors.lastname: Arai
authors.forename: Jun
authors.initials: J
authors.lastname: Ishii
authors.forename: Chiaki
authors.initials: C
authors.lastname: Ogino
authors.forename: Hideki
authors.initials: H
authors.lastname: Fukuda
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kondo
mesh_terms: D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D003296:Cooking; D005087:Glucan 1,4-alpha-Glucosidase / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism*; D012275:Oryza / Q000737:chemistry / Q000378:metabolism*; D010316:Particle Size; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D013213:Starch / Q000378:metabolism; D013696:Temperature
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D013213:Starch; D005087:Glucan 1,4-alpha-Glucosidase; D005978:Glutathione; D005947:Glucose