title: Transcriptional code and disease map for adult retinal cell types.
abstract: Brain circuits are assembled from a large variety of morphologically and functionally diverse cell types. It is not known how the intermingled cell types of an individual adult brain region differ in their expressed genomes. Here we describe an atlas of cell type transcriptomes in one brain region, the mouse retina. We found that each adult cell type expressed a specific set of genes, including a unique set of transcription factors, forming a 'barcode' for cell identity. Cell type transcriptomes carried enough information to categorize cells into morphological classes and types. Several genes that were specifically expressed in particular retinal circuit elements, such as inhibitory neuron types, are associated with eye diseases. The resource described here allows gene expression to be compared across adult retinal cell types, experimenting with specific transcription factors to differentiate stem or somatic cells to retinal cell types, and predicting cellular targets of newly discovered disease-associated genes.
authors.affiliation: Neural Circuit Laboratories, Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.
authors.forename: Sandra
authors.initials: S
authors.lastname: Siegert
authors.forename: Erik
authors.initials: E
authors.lastname: Cabuy
authors.forename: Brigitte Gross
authors.initials: BG
authors.lastname: Scherf
authors.forename: Hubertus
authors.initials: H
authors.lastname: Kohler
authors.forename: Satchidananda
authors.initials: S
authors.lastname: Panda
authors.forename: Yun-Zheng
authors.initials: YZ
authors.lastname: Le
authors.forename: Hans Jörg
authors.initials: HJ
authors.lastname: Fehling
authors.forename: Dimos
authors.initials: D
authors.lastname: Gaidatzis
authors.forename: Michael B
authors.initials: MB
authors.lastname: Stadler
authors.forename: Botond
authors.initials: B
authors.lastname: Roska
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D017630:Connexins / Q000235:genetics; D005136:Eye Proteins / Q000235:genetics / Q000378:metabolism; D005434:Flow Cytometry; D015870:Gene Expression / Q000235:genetics; D015723:Gene Library; D008564:Membrane Potentials / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D046228:Microarray Analysis / Q000379:methods; D009154:Mutation / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000145:classification* / Q000502:physiology*; D018408:Patch-Clamp Techniques; D024642:Potassium Channels, Voltage-Gated / Q000235:genetics; D018092:Kainic Acid Receptors / Q000235:genetics; D012160:Retina / Q000166:cytology*; D012164:Retinal Diseases / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014795:Visual Pathways / Q000378:metabolism; D000097806:GluK2 Kainate Receptor
chemical_list: D017630:Connexins; D005136:Eye Proteins; D009419:Nerve Tissue Proteins; D024642:Potassium Channels, Voltage-Gated; D018092:Kainic Acid Receptors; D014157:Transcription Factors; D000097806:GluK2 Kainate Receptor; C119811:Gja10 protein, mouse