title: Lymphoid progenitors in normal mouse lymph nodes develop into NK cells and T cells in vitro and in vivo.
abstract: We have identified a population of normal mouse LN cells, termed LN lymphoid progenitor (LNLP), resembling common lymphoid progenitor (CLP) in the bone marrow. LNLPs lack lineage markers and express CD127, low levels of CD117 (c-Kit), and Sca-1, but lack fms-related tyrosine kinase 3. They efficiently differentiate in vitro into natural killer (NK) cells and T cells, but not mature B cells. LNLPs injected into nonirradiated lymphopenic mice that have no LN develop into mostly splenic T cells with low numbers of NK cells and B cells. When injected into irradiated mice, they generate NK cells and T cells, but not B cells, in the LN. By contrast, bone marrow CLPs develop into mostly B cells with very small numbers of T and NK cells in recipients' spleen and LN. LNLPs have NK and T-cell potentials, but little B-cell potential, and they can develop into NK cells within the LN of normal mice, but their contribution to the T-cell lineage is unknown.
authors.affiliation: Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, British Columbia, Canada.
authors.forename: Kathrin
authors.initials: K
authors.lastname: Warner
authors.forename: Claudia
authors.initials: C
authors.lastname: Luther
authors.forename: Fumio
authors.initials: F
authors.lastname: Takei
mesh_terms: D000818:Animals; D000950:Antigens, Ly / Q000032:analysis; D001402:B-Lymphocytes / Q000166:cytology; D001853:Bone Marrow; D019070:Cell Lineage; D002478:Cells, Cultured / Q000166:cytology; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D053699:Interleukin-7 Receptor alpha Subunit / Q000032:analysis; D007694:Killer Cells, Natural / Q000166:cytology*; D008198:Lymph Nodes / Q000166:cytology*; D038041:Lymphopoiesis / Q000502:physiology*; D008565:Membrane Proteins / Q000032:analysis; D051379:Mice; D008810:Mice, Inbred C57BL; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D019009:Proto-Oncogene Proteins c-kit / Q000032:analysis; D011828:Radiation Chimera; D013047:Specific Pathogen-Free Organisms; D013601:T-Lymphocytes / Q000166:cytology*
chemical_list: D000950:Antigens, Ly; D053699:Interleukin-7 Receptor alpha Subunit; C074650:Ly6a protein, mouse; D008565:Membrane Proteins; D019009:Proto-Oncogene Proteins c-kit