title: The rhizospheres of traditional medicinal plants in Panxi, China, host a diverse selection of actinobacteria with antimicrobial properties.
abstract: Actinobacteria are a prolific source of antibiotics. Since the rate of discovery of novel antibiotics is decreasing, actinobacteria from unique environments need to be explored. In particular, actinobacterial biocontrol strains from medicinal plants need to be studied as they can be a source of potent antibiotics. We combined culture-dependent and culture-independent methods in analyzing the actinobacterial diversity in the rhizosphere of seven traditional medicinal plant species from Panxi, China, and assessed the antimicrobial activity of the isolates. Each of the plant species hosted a unique set of actinobacterial strains. Out of the 64 morphologically distinct isolates, half were Streptomyces sp., eight were Micromonospora sp., and the rest were members of 18 actinobacterial genera. In particular, Ainsliaea henryi Diels. hosted a diverse selection of actinobacteria, although the 16S ribosomal RNA (rRNA) sequence identity ranges of the isolates and of the 16S rRNA gene clone library were not congruent. In the clone library, 40% of the sequences were related to uncultured actinobacteria, emphasizing the need to develop isolation methods to assess the full potential of the actinobacteria. All Streptomyces isolates showed antimicrobial activity. While the antimicrobial activities of the rare actinobacteria were limited, the growth of Escherichia coli, Verticillium dahliae, and Fusarium oxysporum were inhibited only by rare actinobacteria, and strains related to Saccharopolyspora shandongensis and Streptosporangium roseum showed broad antimicrobial activity.
authors.affiliation: Department of Microbiology, College of Resource and Environmental Sciences, Sichuan Agricultural University, Yaan 625000, People’s Republic of China.
authors.forename: Ke
authors.initials: K
authors.lastname: Zhao
authors.forename: Petri
authors.initials: P
authors.lastname: Penttinen
authors.forename: Qiang
authors.initials: Q
authors.lastname: Chen
authors.forename: Tongwei
authors.initials: T
authors.lastname: Guan
authors.forename: Kristina
authors.initials: K
authors.lastname: Lindström
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Ao
authors.forename: Lili
authors.initials: L
authors.lastname: Zhang
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Zhang
mesh_terms: D039903:Actinobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D000890:Anti-Infective Agents / Q000378:metabolism*; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010802:Phylogeny; D010946:Plants, Medicinal / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D000890:Anti-Infective Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S