title: Adjuvanticity of compound polysaccharides on chickens against Newcastle disease and avian influenza vaccine.
abstract: This study was conducted to evaluate the effects of compound polysaccharides (cPS) on the immune responses via chicken models. First, in screening experiment, a comprehensive analysis for immunomodulatory activity of four cPSs, including Astragalus polysaccharides (APS), Epimedium polysaccharides (EPS), sulfated APS (sAPS) and sulfated EPS (sEPS), was performed in vitro and in vivo. APS-sEPS was picked out having the best effect on lymphocyte proliferation and raising the antibody titers. Therefore, the adjuvanticities of APS-sEPS on Newcastle disease (ND) and avian influenza (AI) vaccine were further validated. Chickens were administrated with ND or AI vaccines containing APS-sEPS of 150, 100 and 50 mg/kg, respectively, taking oil adjuvant vaccine as control. It was observed ND or AI antibody titers and lymphocyte proliferation were enhanced at 100 mg/kg of APS-sEPS. In conclusion, appropriate dose of APS-sEPS may be a safe and efficacious immune stimulator candidate suitable for vaccines.
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Liwei
authors.initials: L
authors.lastname: Guo
authors.forename: Deyun
authors.initials: D
authors.lastname: Wang
authors.forename: Yuanliang
authors.initials: Y
authors.lastname: Hu
authors.forename: Xiaona
authors.initials: X
authors.lastname: Zhao
authors.forename: Yuanlei
authors.initials: Y
authors.lastname: Wang
authors.forename: Shujuan
authors.initials: S
authors.lastname: Yang
authors.forename: Junmin
authors.initials: J
authors.lastname: Wang
authors.forename: Yunpeng
authors.initials: Y
authors.lastname: Fan
authors.forename: Guocai
authors.initials: G
authors.lastname: Han
authors.forename: Huan
authors.initials: H
authors.lastname: Gao
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D000906:Antibodies / Q000276:immunology; D049109:Cell Proliferation; D002645:Chickens; D055808:Drug Discovery; D007111:Immunity, Cellular / Q000276:immunology; D056724:Immunity, Humoral / Q000276:immunology; D007252:Influenza Vaccines / Q000276:immunology*; D005585:Influenza in Birds / Q000276:immunology*; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology; D009521:Newcastle Disease / Q000276:immunology*; D011134:Polysaccharides / Q000737:chemistry* / Q000276:immunology*; D012449:Safety
chemical_list: D000276:Adjuvants, Immunologic; D000906:Antibodies; D007252:Influenza Vaccines; D011134:Polysaccharides