title: A technique for the intra-gastric administration of live larvae of Anisakis simplex in mice.
abstract: To understand the mechanisms of infection and to attempt to simulate human infection by the Anisakidae family, many in vivo experimental approaches have been developed. The aim was to develop and present a technique for the induction of an oral infection through the use of an intra-gastric gavage of live Anisakis simplex in mice. A commercial pediatric gastric tube (No. 4) was cut longitudinally to produce a 3-cm slit at the distal end where the larva was placed to then be administered to the stomach of the mouse. There were no abnormal clinical complications before, during or after the procedure. In conclusion oral infection through the direct delivery of larvae in the stomach is simple and effective.
authors.affiliation: Departamento Materno-Infantil, Faculdade de Medicina, Hospital Universitário Antonio Pedro, Universidade Federal Fluminense, Av. Marques do Paraná, 303, CEP: 24030-210 Centro, Niterói, Rio de Janeiro, Brazil. ifigueiredo@id.uff.br
authors.forename: Israel
authors.initials: I
authors.lastname: Figueiredo
authors.forename: Luciana
authors.initials: L
authors.lastname: Cardoso
authors.forename: Gerlinde
authors.initials: G
authors.lastname: Teixeira
authors.forename: Leila
authors.initials: L
authors.lastname: Lopes
authors.forename: Sergio Carmona
authors.initials: SC
authors.lastname: São Clemente
authors.forename: Mauricio Afonso
authors.initials: MA
authors.lastname: Vericimo
mesh_terms: D000768:Anesthesia, General / Q000379:methods; D000818:Animals; D017129:Anisakiasis / Q000635:transmission*; D017163:Anisakis / Q000502:physiology*; D004195:Disease Models, Animal; D007279:Injections, Subcutaneous; D007441:Intubation, Gastrointestinal; D007814:Larva; D051379:Mice; D008807:Mice, Inbred BALB C; D010274:Parasitology / Q000379:methods*; D010865:Pilot Projects; D011446:Prospective Studies; D013270:Stomach / Q000469:parasitology*