title: Immunohistochemical characterization of nonhuman primate ovarian sex cord-stromal tumors.
abstract: This study evaluates the immunoreactivity of 12 sex cord-stromal tumors of nonhuman primates (11 granulosa cell tumors and 1 luteoma). The markers selected are used in the characterization of gonadal tumors in dogs and other species, including cytokeratins AE1/AE3, GATA-4, inhibin-α, neuron-specific enolase, protein gene product 9.5, and vimentin. A normal nonhuman primate ovary was used as a control and to optimize immunolabeling. Staining was graded as follows: 0 (nonstaining), 1+ (< 10% positive cells), 2+ (10%-50% positive cells), and 3+ (> 50% positive cells). Calretinin, GATA-4, neuron-specific enolase, and vimentin were the most consistently expressed markers (12 of 12). Cytokeratins AE1/AE3 were also consistently expressed (11 of 12). Inhibin-α and protein gene product 9.5 were expressed in 8 and 10 sex cord-stromal tumors, respectively. Results indicate that immunoreactivity of nonhuman primate sex cord-stromal tumors is similar to that observed in other species and that calretinin, GATA-4, and neuron-specific enolase are the most consistently expressed markers in nonhuman primate sex cord-stromal tumors.
authors.affiliation: Department of Comparative Pathobiology, Purdue University, 406 South University, West Lafayette, IN 47907, USA.
authors.forename: A
authors.initials: A
authors.lastname: Durkes
authors.forename: M
authors.initials: M
authors.lastname: Garner
authors.forename: C
authors.initials: C
authors.lastname: Juan-Sallés
authors.forename: J
authors.initials: J
authors.lastname: Ramos-Vara
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis / Q000378:metabolism*; D064032:Calbindin 2 / Q000032:analysis / Q000378:metabolism; D004285:Dogs; D005260:Female; D006106:Granulosa Cell Tumor / Q000378:metabolism / Q000473:pathology / Q000662:veterinary; D006801:Humans; D007150:Immunohistochemistry / Q000662:veterinary; D018311:Luteoma / Q000378:metabolism / Q000473:pathology / Q000662:veterinary; D010051:Ovarian Neoplasms / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D010751:Phosphopyruvate Hydratase / Q000032:analysis / Q000378:metabolism; D018419:Primate Diseases / Q000378:metabolism / Q000473:pathology*; D011323:Primates; D018312:Sex Cord-Gonadal Stromal Tumors / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*
chemical_list: D014408:Biomarkers, Tumor; D064032:Calbindin 2; D010751:Phosphopyruvate Hydratase