title: Genetic and biochemical characterization of a novel metallo-β-lactamase, TMB-1, from an Achromobacter xylosoxidans strain isolated in Tripoli, Libya.
abstract: An Achromobacter xylosoxidans strain from the Tripoli central hospital produced a unique metallo-β-lactamase, designated TMB-1, which is related to DIM-1 (62%) and GIM-1 (51%). bla(TMB-1) was embedded in a class 1 integron and located on the chromosome. The TMB-1 β-lactamase has lower k(cat) values than both DIM-1 and GIM-1 with cephalosporins and carbapenems. The K(m) values were more similar to those of GIM-1 than those of DIM-1, with the overall k(cat)/K(m) values being lower than those for GIM-1 and DIM-1.
authors.affiliation: Cardiff University, Cardiff, United Kingdom.
authors.forename: Allaaeddin
authors.initials: A
authors.lastname: El Salabi
authors.forename: Pardha Saradhi
authors.initials: PS
authors.lastname: Borra
authors.forename: Mark A
authors.initials: MA
authors.lastname: Toleman
authors.forename: Ørjan
authors.initials: Ø
authors.lastname: Samuelsen
authors.forename: Timothy R
authors.initials: TR
authors.lastname: Walsh
mesh_terms: D042441:Achromobacter denitrificans / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000627:therapeutic use; D015780:Carbapenems / Q000008:administration & dosage / Q000627:therapeutic use; D002511:Cephalosporins / Q000008:administration & dosage / Q000627:therapeutic use; D002876:Chromosomes, Bacterial / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics*; D016905:Gram-Negative Bacterial Infections / Q000188:drug therapy / Q000382:microbiology*; D006801:Humans; D032023:Integrons / Q000235:genetics; D007700:Kinetics; D008002:Libya; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D011550:Pseudomonas aeruginosa / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D001618:beta-Lactamases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D015780:Carbapenems; D002511:Cephalosporins; D004269:DNA, Bacterial; D001618:beta-Lactamases; C495217:beta-lactamase GIM-1, Pseudomonas aeruginosa