title: Proteomic analysis of differentially accumulated proteins during ripening and in response to 1-MCP in papaya fruit.
abstract: Papaya (Carica papaya L.) is a climacteric fruit susceptible to postharvest losses due to the ethylene-induced ripening. The inhibitor of ethylene action, 1-methylcyclopropene (1-MCP), has been used worldwide as a safe postharvest non-toxic agent, but the physiological and biochemical modifications induced by 1-MCP are not well understood. Using the 2-DE analysis, we report the changes in the protein profiles after 6 and 18 days of postharvest and the effect of the effect of 1-MCP treatment on fruits. Twenty seven protein spots showing differences in abundance during ripening were successfully identified by nano-LC-ESI/MS/MS. Some spots corresponded to the cell wall degrading enzymes related to fruit ripening; others were involved in oxidative damage protection, protein folding, and cell growth and survival that were induced by 1-MCP. This is the first proteomic report analyzing the effect of 1-MCP in papaya ripening. The present data will help to shed light on papaya fruit ripening process.
authors.affiliation: IPICyT, Instituto Potosino de Investigación Científica y Tecnológica, Camino a la Presa San José No. 2055, Lomas 4ª sección, 78216 San Luis Potosí, SLP, Mexico.
authors.forename: José Ángel
authors.initials: JÁ
authors.lastname: Huerta-Ocampo
authors.forename: Juan Alberto
authors.initials: JA
authors.lastname: Osuna-Castro
authors.forename: Gisela Jareth
authors.initials: GJ
authors.lastname: Lino-López
authors.forename: Alberto
authors.initials: A
authors.lastname: Barrera-Pacheco
authors.forename: Guillermo
authors.initials: G
authors.lastname: Mendoza-Hernández
authors.forename: Antonio
authors.initials: A
authors.lastname: De León-Rodríguez
authors.forename: Ana Paulina
authors.initials: AP
authors.lastname: Barba de la Rosa
mesh_terms: D029441:Carica / Q000378:metabolism*; D003521:Cyclopropanes / Q000494:pharmacology*; D005638:Fruit / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D013997:Time Factors
chemical_list: D003521:Cyclopropanes; D010940:Plant Proteins; D020543:Proteome; C412563:1-methylcyclopropene