title: [Obtaining of fluorescent-labeled nodule bacteria strains of wild legumes for their detection in vive and in vitro].
abstract: A series of expression vectors containing genes of fluorescent proteins TurboGFP and TurboRFP under the phage T5 constitutive promoter regulation, intended for lifetime marking of nodule bacteria is created: a series of vectors based on a broad-host-range replicon BBRI, for marking strains with an expression of reporter gene from a transformed plasmid and a series of vectors based on a plasmid pRL765gfp for marking strains by introduction genes of fluorescent proteins in a bacterial chromosome. It was shown that transformation is the most preferable method of constructions transfer in nodule bacteria cells, as in the presence of mob locus in the vectors necessary for conjugation, exists the possibility of occasional plasmid mobilization and its transition from marked strain cells in other soil bacteria. With application of the created vector constructions we obtained fluorescent tagged strains of Rhizobium sp., Mesorhizobium sp., Ensifer (Sinorhizobium) sp., Bradyrhizobium sp., Phyllobacterium sp., Agrobacterium sp. Also their suitability for experiments in vivo and in vitro is shown.
authors.forename: A Kh
authors.initials: AKh
authors.lastname: Baĭmiev
authors.forename: R S
authors.initials: RS
authors.lastname: Iamidanov
authors.forename: R T
authors.initials: RT
authors.lastname: Matniiazov
authors.forename: D K
authors.initials: DK
authors.lastname: Blagova
authors.forename: A Kh
authors.initials: AKh
authors.lastname: Baĭmiev
authors.forename: A V
authors.initials: AV
authors.lastname: Chemeris
mesh_terms: D060054:Agrobacterium / Q000235:genetics / Q000302:isolation & purification; D020369:Bradyrhizobium / Q000235:genetics / Q000302:isolation & purification; D002876:Chromosomes, Bacterial; D007887:Fabaceae / Q000145:classification / Q000382:microbiology*; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D008164:Luminescent Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D059128:Mesorhizobium / Q000235:genetics / Q000302:isolation & purification; D059127:Phyllobacteriaceae / Q000235:genetics / Q000302:isolation & purification; D012231:Rhizobium / Q000235:genetics / Q000302:isolation & purification*; D053204:Root Nodules, Plant / Q000382:microbiology*; D020579:Sinorhizobium / Q000235:genetics / Q000302:isolation & purification; D000097573:Red Fluorescent Protein
chemical_list: D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein