title: Anticancer efficacy of perillyl alcohol-bearing PLGA microparticles.
abstract: In the present study, a novel poly-lactic glycolic acid (PLGA)-based microparticle formulation of perillyl alcohol (POH) was prepared and characterized. Further, its efficacy was evaluated against di-methyl benzo anthracene-induced skin papilloma in Swiss albino mice. The characterization studies showed that POH-bearing PLGA microparticles were of the size 768 ± 215 nm with a ζ-potential value of -7.56 ± 0.88 mV. The entrapment efficiency of the active drug in particles was 42.4% ± 3.5%. POH-bearing PLGA microparticles were stable and released entrapped drug gradually over an extended time period. The in vitro efficacy of POH-bearing PLGA microparticles was evaluated by examining their differential cytotoxicity and assessing their ability to inhibit epidermoid carcinoma cell line (A253). The POH-based microparticles when administered to tumor-bearing animals caused greater tumor regression and increased survival rate (∼80%) as compared with the group receiving free form of POH (survival rate 40%). The superiority of POH-PLGA microparticles over free form of POH was further evident from their ability to modulate apoptosis-regulating factors.
authors.affiliation: Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, Uttar Pradesh, India.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Farazuddin
authors.forename: Bhawna
authors.initials: B
authors.lastname: Sharma
authors.forename: Aijaz Ahmed
authors.initials: AA
authors.lastname: Khan
authors.forename: Beenu
authors.initials: B
authors.lastname: Joshi
authors.forename: Mohammad
authors.initials: M
authors.lastname: Owais
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D051017:Apoptosis Regulatory Proteins; D045744:Cell Line, Tumor; D005260:Female; D053208:Kaplan-Meier Estimate; D019344:Lactic Acid / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D008863:Microspheres; D039821:Monoterpenes / Q000737:chemistry / Q000494:pharmacology*; D010212:Papilloma / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D011100:Polyglycolic Acid / Q000737:chemistry / Q000494:pharmacology*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D012878:Skin Neoplasms / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D023041:Xenograft Model Antitumor Assays
chemical_list: D000970:Antineoplastic Agents; D051017:Apoptosis Regulatory Proteins; D039821:Monoterpenes; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; C032208:perillyl alcohol; D019344:Lactic Acid