title: Circular permutation of the starch-binding domain: inversion of ligand selectivity with increased affinity.
abstract: Proteins containing starch-binding domains (SBDs) are used in a variety of scientific and technological applications. A circularly permutated SBD (CP90) with improved affinity and selectivity toward longer-chain carbohydrates was synthesized, suggesting that a new starch-binding protein may be developed for specific scientific and industrial applications.
authors.affiliation: Institute of Bioinformatics and Structural Biology, National Tsing Hua University, No. 101, Sec. 2, Kuang Fu Rd, Hsinchu, 30013, Taiwan ROC.
authors.forename: Preyesh
authors.initials: P
authors.lastname: Stephen
authors.forename: Kai-Li
authors.initials: KL
authors.lastname: Tseng
authors.forename: Yu-Nan
authors.initials: YN
authors.lastname: Liu
authors.forename: Ping-Chiang
authors.initials: PC
authors.lastname: Lyu
mesh_terms: D000688:Amylose / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D049452:Green Fluorescent Proteins / Q000737:chemistry* / Q000378:metabolism; D008024:Ligands; D008958:Models, Molecular; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D013213:Starch / Q000737:chemistry* / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: C485182:Cyan Fluorescent Protein; D008024:Ligands; D049452:Green Fluorescent Proteins; D013213:Starch; D000688:Amylose