title: Effect of inhibition of the adrenomedullin gene on the growth and chemosensitivity             of ovarian cancer cells.
abstract: Adrenomedullin (AM), a potent vasodilator peptide, is present in various             types of tumors. Here, we constructed short hairpin RNA (shRNA) in order to target             the AM gene in vitro using RNA interference (RNAi) technology. HO8910 ovarian             cancer cells were transfected, and the effects of AM on proliferation and chemosensitivity             of the cells were examined. RT-PCR, real-time PCR and western blot analysis were             performed to detect the AM gene and protein expression. The MTT assay was used             to observe the effect of AM on proliferation and chemosensitivity of the cells.             Also, the protein levels of bcl-2 and the extracellular regulated protein kinase             (ERK) were evaluated by western blot analysis. We found that silencing of the             AM gene inhibited the proliferation and increased the chemosensitivity of HO8910             cells, reduced the expression of AM mRNA and protein as well as downregulated             bcl-2 and p-ERK expression. We, therefore, conclude that silencing of the AM gene             in HO8910 ovarian cancer cells inhibited the proliferation and increased the chemosensitivity             of the cells through downregulation of ERK and bcl-2 expression. Thus, anti-AM             treatment together with suppression of ERK and bcl-2 expression provides a novel             research approach for ovarian cancer.
authors.affiliation: Department of Gynecology, the First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning, PR China.
authors.forename: Pan
authors.initials: P
authors.lastname: Chen
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Pang
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yunqin
authors.initials: Y
authors.lastname: He
mesh_terms: D053607:Adrenomedullin / Q000235:genetics*; D000970:Antineoplastic Agents / Q000494:pharmacology; D001483:Base Sequence; D016190:Carboplatin / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D023061:Gene Order; D020868:Gene Silencing; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008969:Molecular Sequence Data; D010051:Ovarian Neoplasms / Q000188:drug therapy / Q000235:genetics*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D034741:RNA, Small Interfering / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D019253:Proto-Oncogene Proteins c-bcl-2; D034741:RNA, Small Interfering; D053607:Adrenomedullin; D016190:Carboplatin