title: Modulation of central corneal thickness by various riboflavin eyedrop compositions in porcine corneas.
abstract: OBJECTIVE
  To evaluate the modulatory effect of various riboflavin 0.1% and 0.2% compositions on the central corneal thickness (CCT) in fresh porcine corneas.
  
  
  METHODS
  Department of Ophthalmology, Johannes Gutenberg University of Mainz, Mainz, Germany.
  
  
  METHODS
  Experimental study.
  
  
  METHODS
  The CCT in freshly enucleated porcine globes was measured by ultrasound pachymetry before and after (if applicable) deepithelialization and every 10 minutes thereafter during 120 minutes of eyedrop application. In Groups 1 and 2 (controls), no eyedrops were applied. In Groups 3 and 4, isotonic riboflavin eyedrops were used. In Groups 5 to 9, hypotonic riboflavin eyedrops were given. In Groups 10 and 11, preparations for transepithelial crosslinking were applied. In Groups 2 to 9, deepithelialization was performed. The final CCT in the groups was compared by analysis of variance.
  
  
  RESULTS
  One hundred ten freshly enucleated porcine globes were used. The mean final CCT compared with preoperative values was 97% ± 4% (SD) in Group 1, 91% ± 4% in Group 2, 66% ± 5% in Group 3, 151% ± 13% in Group 4, 65% ± 2% in Group 5, 105% ± 3% in Group 6, 120% ± 4% in Group 7, 130% ± 4% in Group 8, 132% ± 4% in Group 9, 114% ± 2% in Group 10, and 114% ± 4% in Group 11. The differences between Group 1 and each of Groups 3, 4, 5, 7, 8, and 9 were statistically significant (P<.05).
  
  
  CONCLUSIONS
  There was considerable variation in the final CCT as a result of varying riboflavin eyedrop compositions.
  
  
  BACKGROUND
  No author has a financial or proprietary interest in any material or method mentioned.
authors.affiliation: Department of Ophthalmology, University Medical Center, Johannes Gutenberg University Mainz, Mainz, Germany. jan.vetter@unimedizin-mainz.de
authors.forename: Jan M
authors.initials: JM
authors.lastname: Vetter
authors.forename: Stephan
authors.initials: S
authors.lastname: Brueckner
authors.forename: Marija
authors.initials: M
authors.lastname: Tubic-Grozdanis
authors.forename: Urs
authors.initials: U
authors.lastname: Vossmerbäumer
authors.forename: Norbert
authors.initials: N
authors.lastname: Pfeiffer
authors.forename: Sabine
authors.initials: S
authors.lastname: Kurz
mesh_terms: D000818:Animals; D001837:Body Weights and Measures; D003315:Cornea / Q000000981:diagnostic imaging* / Q000187:drug effects* / Q000473:pathology; D015715:Corneal Edema / Q000000981:diagnostic imaging / Q000517:prevention & control*; D003432:Cross-Linking Reagents; D006863:Hydrogen-Ion Concentration; D009883:Ophthalmic Solutions; D009994:Osmolar Concentration; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000008:administration & dosage*; D012256:Riboflavin / Q000008:administration & dosage*; D013552:Swine; D014463:Ultrasonography
chemical_list: D003432:Cross-Linking Reagents; D009883:Ophthalmic Solutions; D017319:Photosensitizing Agents; D012256:Riboflavin