title: The influence of presaccharification, fermentation temperature and yeast strain on ethanol production from sugarcane bagasse.
abstract: Ethanol can be produced from cellulosic biomass in a process known as simultaneous saccharification and fermentation (SSF). The presence of yeast together with the cellulolytic enzyme complex reduces the accumulation of sugars within the reactor, increasing the ethanol yield and saccharification rate. This paper reports the isolation of Saccharomyces cerevisiae LBM-1, a strain capable of growth at 42 °C. In addition, S. cerevisiae LBM-1 and Kluyveromyces marxianus UFV-3 were able to ferment sugar cane bagasse in SSF processes at 37 and 42 °C. Higher ethanol yields were observed when fermentation was initiated after presaccharification at 50°C than at 37 or 42° C. Furthermore, the volumetric productivity of fermentation increased with presaccharification time, from 0.43 g/L/h at 0 h to 1.79 g/L/h after 72 h of presaccharification. The results suggest that the use of thermotolerant yeasts and a presaccharification stage are key to increasing yields in this process.
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Av. PH Rolfs s/n, Campus Universitário, Viçosa 36571-000, MG, Brazil.
authors.forename: Carlos J A
authors.initials: CJ
authors.lastname: de Souza
authors.forename: Daniela A
authors.initials: DA
authors.lastname: Costa
authors.forename: Marina Q R B
authors.initials: MQ
authors.lastname: Rodrigues
authors.forename: Ancély F
authors.initials: AF
authors.lastname: dos Santos
authors.forename: Mariana R
authors.initials: MR
authors.lastname: Lopes
authors.forename: Aline B P
authors.initials: AB
authors.lastname: Abrantes
authors.forename: Patrícia
authors.initials: P
authors.lastname: dos Santos Costa
authors.forename: Wendel Batista
authors.initials: WB
authors.lastname: Silveira
authors.forename: Flávia M L
authors.initials: FM
authors.lastname: Passos
authors.forename: Luciano G
authors.initials: LG
authors.lastname: Fietto
mesh_terms: D001709:Biotechnology / Q000379:methods*; D050260:Carbohydrate Metabolism; D002482:Cellulose / Q000737:chemistry; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation / Q000502:physiology*; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis; D007716:Kluyveromyces / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D031786:Saccharum / Q000737:chemistry*; D013696:Temperature; D013997:Time Factors; D015003:Yeasts / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*
chemical_list: D000431:Ethanol; D002482:Cellulose; C027433:bagasse; D005947:Glucose