title: The histone phosphatase inhibitory property of plant nucleosome assembly protein-related proteins (NRPs).
abstract: SET/I(2)(PP2A), a member of the family of nucleosome assembly proteins (NAPs), has been previously described as a multifunctional protein inhibiting protein phosphatase 2A (PP2A)-mediated histone H3((pSer10)) dephosphorylation during the heat shock response in animal cells. In the present work we demonstrate that its plant orthologs, designated as NAP-related proteins (NRPs), have a similar in vitro biochemical activity and interact with PP2A and histone H3((pSer10))in vivo. Although heat shock gene promoters were found to be associated with histone H3((pSer10))-marked chromatin following a high temperature treatment, heat shock gene expression was not affected in NRP-deficient mutant Arabidopsis thaliana (L.) plantlets. These observations indicate that NRPs are potential regulators of histone dephosphorylation in plants, but they are dispensable for gene expression reorganization in response to heat shock.
authors.affiliation: Institute of Plant Biology, Biological Research Center, Hungarian Academy of Sciences, Temesvári krt. 62, H-6726 Szeged, Hungary.
authors.forename: Judit
authors.initials: J
authors.lastname: Bíró
authors.forename: Ilona
authors.initials: I
authors.lastname: Farkas
authors.forename: Mónika
authors.initials: M
authors.lastname: Domoki
authors.forename: Krisztina
authors.initials: K
authors.lastname: Otvös
authors.forename: Sándor
authors.initials: S
authors.lastname: Bottka
authors.forename: Viktor
authors.initials: V
authors.lastname: Dombrádi
authors.forename: Attila
authors.initials: A
authors.lastname: Fehér
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D042002:Chromatin Assembly and Disassembly; D004791:Enzyme Inhibitors; D015870:Gene Expression; D006360:Heat-Shock Proteins / Q000235:genetics*; D006657:Histones / Q000235:genetics / Q000378:metabolism; D006358:Hot Temperature; D000455:Medicago sativa / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D009707:Nucleosomes / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D054648:Protein Phosphatase 2 / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D016415:Sequence Alignment
chemical_list: D000906:Antibodies; D004791:Enzyme Inhibitors; D006360:Heat-Shock Proteins; D006657:Histones; D009707:Nucleosomes; D010940:Plant Proteins; D054648:Protein Phosphatase 2