title: Population dynamics and ORF3 gene evolution of porcine circovirus type 2 circulating in Korea.
abstract: This study was conducted to investigate the status and population dynamics of porcine circovirus type 2(PCV2) in Korea and to assess the molecular evolutionary pattern of the two biologically important, overlapping open reading frames, the ORF1 and ORF3 genes. A wide range of PCV2 genomic sequences (entire genome, ORF1, ORF2 and ORF3) collected between 2001 and 2010 were analyzed using the Bayesian Markov chain Monte Carlo and maximum-likelihood approaches. These techniques identified the PCV2d genotype and the 2Ek cluster of PCV2a in Korea for the first time. Second, the genotypic shift of PCV2b dominating over PCV2a likely occurred between 2002 and 2004 due to a population expansion of PCV2b. In the context of positive Darwinian selection, the results uncovered independent evolutionary patterns in the ORF3 gene compared to the overlapping ORF1 gene and new sites in the viral ORFs/proteins that might relate to differences in the biological properties of the PCV2 groups.
authors.affiliation: Department of Veterinary Medicine Virology Laboratory, College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, Seoul 151-742, Korea.
authors.forename: Van Giap
authors.initials: VG
authors.lastname: Nguyen
authors.forename: Hye Kwon
authors.initials: HK
authors.lastname: Kim
authors.forename: Hyoung Joon
authors.initials: HJ
authors.lastname: Moon
authors.forename: Seong Jun
authors.initials: SJ
authors.lastname: Park
authors.forename: Hyun Ok
authors.initials: HO
authors.lastname: Keum
authors.forename: Semi
authors.initials: S
authors.lastname: Rho
authors.forename: Jae Yeon
authors.initials: JY
authors.lastname: Han
authors.forename: Bong Kyun
authors.initials: BK
authors.lastname: Park
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018173:Circoviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D019143:Evolution, Molecular; D005260:Female; D008297:Male; D016366:Open Reading Frames; D010802:Phylogeny; D056910:Republic of Korea / Q000453:epidemiology; D016415:Sequence Alignment; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D014764:Viral Proteins