title: Oxidative stress promotes hypertension and albuminuria during the autoimmune disease systemic lupus erythematosus.
abstract: Several lines of evidence suggest that essential hypertension originates from an autoimmune-mediated mechanism. One consequence of chronic immune activation is the generation of oxygen-derived free radicals, resulting in oxidative stress. Renal oxidative stress has direct prohypertensive actions on renal microvascular and tubular function. Whether oxidative stress contributes to the prevalent hypertension associated with autoimmune disease is not clear. We showed previously that female NZBWF1 mice, an established model of the autoimmune disease systemic lupus erythematosus (SLE), develop hypertension associated with renal oxidative stress. In the present study we tested the hypothesis that oxidative stress contributes to autoimmune-mediated hypertension by treating SLE and control (NZW/LacJ) mice with tempol (2.0 mmol/L) and apocynin (1.5 mmol/L) in the drinking water for 4 weeks. Although the treatment did not alter SLE disease activity (assessed by plasma double-stranded DNA autoantibodies), blood pressure and renal injury (urinary albumin) were reduced in the treated SLE mice. Tempol plus apocynin-treated SLE mice had reduced expression of nitrosylated proteins in the renal cortex, as well as reduced urinary and renal cortical hydrogen peroxide, suggesting that treatment reduced renal markers of oxidative stress. These data suggest that renal oxidative stress plays an important mechanistic role in the development of autoimmune-mediated hypertension.
authors.affiliation: Department of Physiology and Biophysics, University of Mississippi Medical Center, Jackson, MS 39216-4505, USA.
authors.forename: Keisa W
authors.initials: KW
authors.lastname: Mathis
authors.forename: Marcia
authors.initials: M
authors.lastname: Venegas-Pont
authors.forename: C Warren
authors.initials: CW
authors.lastname: Masterson
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Stewart
authors.forename: Katie L
authors.initials: KL
authors.lastname: Wasson
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Ryan
mesh_terms: D000098:Acetophenones / Q000627:therapeutic use; D000419:Albuminuria / Q000188:drug therapy / Q000209:etiology* / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use; D015551:Autoimmunity; D001794:Blood Pressure; D003497:Cyclic N-Oxides / Q000627:therapeutic use; D004195:Disease Models, Animal; D018450:Disease Progression; D004357:Drug Synergism; D005260:Female; D005500:Follow-Up Studies; D006973:Hypertension / Q000188:drug therapy / Q000209:etiology* / Q000378:metabolism; D015151:Immunoblotting; D008180:Lupus Erythematosus, Systemic / Q000150:complications* / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008814:Mice, Inbred NZB; D018384:Oxidative Stress; D013113:Spin Labels
chemical_list: D000098:Acetophenones; D000975:Antioxidants; D003497:Cyclic N-Oxides; D013113:Spin Labels; C056165:acetovanillone; C001803:tempol