title: Effective RNA interference in cultured silkworm cells mediated by overexpression of Caenorhabditis elegans SID-1.
abstract: RNA interference (RNAi) is a conserved mechanism that catalyzes sequence-specific gene silencing and has been used for loss-of-function genetic screens in many organisms. Here, we demonstrated that the expression of Caenorhabditis elegans SID-1 (CeSID-1) could trigger effective gene silencing in the cultured silkworm cell line, BmN4 (BmN4-SID1). Soaking the BmN4-SID1 in dsRNA corresponding to endogenous target genes induced a significant decrease of the amount of mRNA or protein. A small amount of dsRNA was enough to silence the target gene in a few days. Overexpression of CeSID-1 did not affect the cell viability. Our results suggest that BmN4-SID1 can be used in many applications in silkworm cells and will become a valuable resource for gene analysis.
authors.affiliation: Laboratory of Silkworm Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences, Hakozaki, Fukuoka, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Mon
authors.forename: Isao
authors.initials: I
authors.lastname: Kobayashi
authors.forename: Shinji
authors.initials: S
authors.lastname: Ohkubo
authors.forename: Shuichiro
authors.initials: S
authors.lastname: Tomita
authors.forename: Jaeman
authors.initials: J
authors.lastname: Lee
authors.forename: Hideki
authors.initials: H
authors.lastname: Sezutsu
authors.forename: Toshiki
authors.initials: T
authors.lastname: Tamura
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kusakabe
mesh_terms: D000818:Animals; D012831:Bombyx / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D017173:Caenorhabditis elegans / Q000235:genetics; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D002470:Cell Survival; D003001:Cloning, Molecular; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014162:Transfection
chemical_list: D029742:Caenorhabditis elegans Proteins; D008565:Membrane Proteins; D012330:RNA, Double-Stranded; D012333:RNA, Messenger; C452203:SID-1 protein, C elegans