title: Application of RNA-Seq transcriptome analysis: CD151 is an Invasion/Migration target in all stages of epithelial ovarian cancer.
abstract: BACKGROUND
  RNA-Seq allows a theoretically unbiased analysis of both genome-wide transcription levels and mutation status of a tumor. Using this technique we sought to identify novel candidate therapeutic targets expressed in epithelial ovarian cancer (EOC).
  
  
  METHODS
  Specifically, we sought candidate invasion/migration targets based on expression levels across all tumors, novelty of expression in EOC, and known function. RNA-Seq analysis revealed the high expression of CD151, a transmembrane protein, across all stages of EOC. Expression was confirmed at both the mRNA and protein levels using RT-PCR and immunohistochemical staining, respectively.
  
  
  RESULTS
  In both EOC tumors and normal ovarian surface epithelial cells we demonstrated CD151 to be localized to the membrane and cell-cell junctions in patient-derived and established EOC cell lines. We next evaluated its role in EOC dissemination using two ovarian cancer-derived cell lines with differential levels of CD151 expression. Targeted antibody-mediated and siRNA inhibition or loss of CD151 in SKOV3 and OVCAR5 cell lines effectively inhibited their migration and invasion.
  
  
  CONCLUSIONS
  Taken together, these findings provide the first proof-of-principle demonstration for a next generation sequencing approach to identifying candidate therapeutic targets and reveal CD151 to play a role in EOC dissemination.
authors.affiliation: Department of Genetics and Genomic Sciences, Mount Sinai School of Medicine, New York, NY, USA. John.Martignetti@mssm.edu.
authors.forename: Rebecca A
authors.initials: RA
authors.lastname: Mosig
authors.forename: Li
authors.initials: L
authors.lastname: Lin
authors.forename: Emir
authors.initials: E
authors.lastname: Senturk
authors.forename: Hardik
authors.initials: H
authors.lastname: Shah
authors.forename: Fei
authors.initials: F
authors.lastname: Huang
authors.forename: Peter
authors.initials: P
authors.lastname: Schlosshauer
authors.forename: Samantha
authors.initials: S
authors.lastname: Cohen
authors.forename: Robert
authors.initials: R
authors.lastname: Fruscio
authors.forename: Sergio
authors.initials: S
authors.lastname: Marchini
authors.forename: Maurizio
authors.initials: M
authors.lastname: D'Incalci
authors.forename: Ravi
authors.initials: R
authors.lastname: Sachidanandam
authors.forename: Peter
authors.initials: P
authors.lastname: Dottino
authors.forename: John A
authors.initials: JA
authors.lastname: Martignetti