title: The ICAM-1 (469 T/C) gene polymorphism but not (241 G/A) is associated with Behcet`s disease in the Lebanese population.
abstract: OBJECTIVE
  To investigate the association of the 2 intracellular adhesion molecules-1 (ICAM-1) gene polymorphisms [thymidine/cytidine (T/C) 469 and guanosine/adenosine (G/A) 241] in Behcet`s disease in Lebanon.
  
  
  METHODS
  We initiated the study in July 2003, and carried out the work in the research laboratory of Beirut Arab University, Beirut, Lebanon. We extracted the DNA by glass fiber matrix mini column. We amplified the ICAM gene by polymerase chain reaction (PCR) and tested the PCR products for the presence of the polymorphisms using a restriction enzyme specific for each polymorphism. We analyzed the results by agarose electrophoresis.
  
  
  RESULTS
  We demonstrated the association of only one single nucleotide polymorphism (SNP) (K469) with Behcet`s disease, while we could not detect the other SNP (G241A) in either controls or patients in the Lebanese population.
  
  
  CONCLUSIONS
  The ICAM-1 gene polymorphism 469 T/C, but not 241 G/A, may encode risk for Behcet`s disease in the Lebanese population.
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, Beirut Arab University, PO Box 115020, Beirut, Lebanon.
authors.forename: Hania N
authors.initials: HN
authors.lastname: Chmaisse
authors.forename: Hana A
authors.initials: HA
authors.lastname: Fakhoury
authors.forename: Nuha N
authors.initials: NN
authors.lastname: Salti
authors.forename: Rajaa F
authors.initials: RF
authors.lastname: Makki