title: Sterols from a soft coral, Dendronephthya gigantea as farnesoid X-activated receptor antagonists.
abstract: Three new steroids 3-oxocholest-1,22-dien-12β-ol (1), 3-oxocholest-1,4-dien-20β-ol (2), 3-oxocholest-1,4-dien-12β-ol (3), and three known steroids (20S)-20-Hydroxyergosta-1,4,24(28)-trien-3-one (4) [7a], 5α,8α-Epidioxycholesta-6,22-dien-3β-ol (5) [10] and 5-cholestene-3β,12β-diol (6) [11] were isolated from a soft coral Dendronephthya gigantea. Their chemical structures and relative stereochemistry were elucidated by the analysis of HRMS and 2-D NMR spectroscopic data. The steroids 1 and 2 showed notable inhibitory activity against farnesoid X-activated receptor (FXR) with IC(50)'s 14 and 15μM.
authors.affiliation: Center for Marine Natural Products and Drug Discovery, School of Earth and Environmental Sciences, Seoul National University, NS-80, Seoul 151-747, Republic of Korea.
authors.forename: Kyoungjin
authors.initials: K
authors.lastname: Shin
authors.forename: Jungwook
authors.initials: J
authors.lastname: Chin
authors.forename: Dongyup
authors.initials: D
authors.lastname: Hahn
authors.forename: Jaehwan
authors.initials: J
authors.lastname: Lee
authors.forename: Hoosang
authors.initials: H
authors.lastname: Hwang
authors.forename: Dong Hwan
authors.initials: DH
authors.lastname: Won
authors.forename: Jungyeob
authors.initials: J
authors.lastname: Ham
authors.forename: Hyukjae
authors.initials: H
authors.lastname: Choi
authors.forename: Eunsoo
authors.initials: E
authors.lastname: Kang
authors.forename: Hiyoung
authors.initials: H
authors.lastname: Kim
authors.forename: Moon Kyeong
authors.initials: MK
authors.lastname: Ju
authors.forename: Sang-Jip
authors.initials: SJ
authors.lastname: Nam
authors.forename: Heonjoong
authors.initials: H
authors.lastname: Kang
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000737:chemistry*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002784:Cholesterol / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D020128:Inhibitory Concentration 50; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D018160:Receptors, Cytoplasmic and Nuclear / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D013261:Sterols / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D014162:Transfection; D000099150:Receptor, Farnesoid X-Activated
chemical_list: D002784:Cholesterol; D018160:Receptors, Cytoplasmic and Nuclear; D013261:Sterols; D000099150:Receptor, Farnesoid X-Activated; C574199:3-oxocholest-1,22-dien-12beta-ol