title: Suppression of nitric oxide production on LPS/IFN-γ-stimulated RAW264.7 macrophages by a novel catechin, pilosanol N, from Agrimonia pilosa Ledeb.
abstract: A novel catechin, pilosanol N (1), was isolated from Agrimonia pilosa Ledeb and its structure was determined by (1)H, (13)C NMR and HRESI-MS analyses. Compound 1 inhibited nitric oxide (NO) production in LPS/IFN-γ -induced RAW264.7 macrophages, and then the iNOS gene expression and its protein production with LPS/IFN-γ treatment cells were also suppressed in the presence of 1. In addition, compound 1 scavenged NO or nitrogen radicals generated from NOR3 (4-ethyl-2-hydroxyamino-5-nitro-3-hexenamide) as an NO donor. These results indicated that pilosanol N can decrease the level of NO through a mechanism that involved both a decrease in the NO production and NO scavenging.
authors.affiliation: Department of Bioresource Technology, Okinawa National College of Technology, Nago City, Okinawa, Japan. taira@okinawa-ct.ac.jp
authors.forename: Junsei
authors.initials: J
authors.lastname: Taira
authors.forename: Wakana
authors.initials: W
authors.lastname: Ohmine
authors.forename: Takayuki
authors.initials: T
authors.lastname: Ogi
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Nanbu
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Ueda
mesh_terms: D031981:Agrimonia / Q000737:chemistry*; D002392:Catechin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D007371:Interferon-gamma / Q000737:chemistry / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000737:chemistry / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000096:biosynthesis*
chemical_list: D008070:Lipopolysaccharides; C572309:pilosanol N; D009569:Nitric Oxide; D007371:Interferon-gamma; D002392:Catechin