title: Mitochondrial ATP-sensitive potassium channel activity and hypoxic preconditioning are independent of an inwardly rectifying potassium channel subunit in Caenorhabditis elegans.
abstract: Hypoxic preconditioning (HP) is an evolutionarily-conserved mechanism that protects an organism against stress. The mitochondrial ATP-sensitive K(+) channel (mK(ATP)) plays an essential role in the protective signaling, but remains molecularly undefined. Several lines of evidence suggest that mK(ATP) may arise from an inward rectifying K(+) channel (Kir). The genetic model organism Caenorhabditis elegans exhibits HP and displays mK(ATP) activity. Here, we investigate the tissue expression profile of the three C. elegans Kir genes and demonstrate that mutant strains where the irk genes have been deleted either individually or in combination can be protected by HP and exhibit robust mK(ATP) channel activity in purified mitochondria. These data suggest that the mK(ATP) in C. elegans does not arise from a Kir derived channel.
authors.affiliation: Department of Medicine, University of Rochester Medical Center, Rochester, NY 14642, USA.
authors.forename: Andrew P
authors.initials: AP
authors.lastname: Wojtovich
authors.forename: Peter
authors.initials: P
authors.lastname: DiStefano
authors.forename: Teresa
authors.initials: T
authors.lastname: Sherman
authors.forename: Paul S
authors.initials: PS
authors.lastname: Brookes
authors.forename: Keith
authors.initials: K
authors.lastname: Nehrke
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D017173:Caenorhabditis elegans / Q000235:genetics / Q000378:metabolism*; D017238:Genes, Helminth; D049452:Green Fluorescent Proteins / Q000235:genetics; D000860:Hypoxia / Q000378:metabolism; D019194:Ischemic Preconditioning; D054086:KATP Channels / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism*; D024661:Potassium Channels, Inwardly Rectifying / Q000378:metabolism; D013312:Stress, Physiological
chemical_list: D054086:KATP Channels; D024661:Potassium Channels, Inwardly Rectifying; D049452:Green Fluorescent Proteins