title: Particle size measurement of lipoprotein fractions using diffusion-ordered NMR spectroscopy.
abstract: The sizes of certain types of lipoprotein particles have been associated with an increased risk of cardiovascular disease. However, there is currently no gold standard technique for the determination of this parameter. Here, we propose an analytical procedure to measure lipoprotein particles sizes using diffusion-ordered nuclear magnetic resonance spectroscopy (DOSY). The method was tested on six lipoprotein fractions, VLDL, IDL, LDL1, LDL2, HDL2, and HDL3, which were obtained by sequential ultracentrifugation from four patients. We performed a pulsed-field gradient experiment on each fraction to obtain a mean diffusion coefficient, and then determined the apparent hydrodynamic radius using the Stokes–Einstein equation. To validate the hydrodynamic radii obtained, the particle size distribution of these lipoprotein fractions was also measured using transmission electron microscopy (TEM). The standard errors of duplicate measurements of diffusion coefficient ranged from 0.5% to 1.3%, confirming the repeatability of the technique. The coefficient of determination between the hydrodynamic radii and the TEM-derived mean particle size was r2 = 0.96, and the agreement between the two techniques was 85%. Thus, DOSY experiments have proved to be accurate and reliable for estimating lipoprotein particle sizes.
authors.affiliation: Department of Electronic Engineering, Universitat Rovira i Virgili, Tarragona, Spain. roger.mallol@urv.cat
authors.forename: Roger
authors.initials: R
authors.lastname: Mallol
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Rodríguez
authors.forename: Mercedes
authors.initials: M
authors.lastname: Heras
authors.forename: Maria
authors.initials: M
authors.lastname: Vinaixa
authors.forename: Núria
authors.initials: N
authors.lastname: Plana
authors.forename: Lluís
authors.initials: L
authors.lastname: Masana
authors.forename: Gareth A
authors.initials: GA
authors.lastname: Morris
authors.forename: Xavier
authors.initials: X
authors.lastname: Correig
mesh_terms: D006801:Humans; D008074:Lipoproteins / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D046529:Microscopy, Electron, Transmission; D010316:Particle Size; D014461:Ultracentrifugation
chemical_list: D008074:Lipoproteins