title: Reduced levels of class 1 reversibly glycosylated polypeptide increase intercellular transport via plasmodesmata.
abstract: Maize and Arabidopsis thaliana class 1 reversibly glycosylated polypeptides (C1RGPs) are plasmodesmata-associated proteins. Previously, over-expression of Arabidopsis C1RGP AtRGP2 in Nicotiana tabacum was shown to reduce intercellular transport of photoassimilate, resulting in stunted, chlorotic plants, and inhibition of local cell-to-cell spread of tobacco mosaic virus (TMV). Here, we used virus induced gene silencing to examine the effects of reduced levels of C1RGPs in Nicotiana benthamiana. Silenced plants show wild-type growth and development. Intercellular transport in silenced plants was probed using fluorescently labeled TMV and its movement protein, P30. P30 shows increased cell-to-cell movement and TMV exhibited accelerated systemic spread compared to control plants. These results support the hypothesis that C1RGPs act to regulate intercellular transport via plasmodesmata.
authors.affiliation: Department of Plant and Microbial Biology, Koshland Hall, University of California, Berkeley, CA, USA.
authors.forename: Tessa M
authors.initials: TM
authors.lastname: Burch-Smith
authors.forename: Ya
authors.initials: Y
authors.lastname: Cui
authors.forename: Patricia C
authors.initials: PC
authors.lastname: Zambryski
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D020868:Gene Silencing; D006031:Glycosylation; D031425:Plasmodesmata / Q000378:metabolism*; D021381:Protein Transport; D014026:Nicotiana / Q000378:metabolism; D014027:Tobacco Mosaic Virus / Q000502:physiology
chemical_list: D029681:Arabidopsis Proteins