title: Models of anxiety: stress-induced hyperthermia (SIH) in singly housed mice.
abstract: Described in this unit is the stress-induced hyperthermia (SIH) test in mice in a single-housed format. This protocol has proven reliable in detecting the anxiolytic properties of test compounds. In this test, SIH is quantified in singly housed mice using a rectal temperature measurement as the stressor. Rectal temperature is measured twice at a 10-min interval. Due to the stress experienced during the first temperature measurement, the temperature of the second measurement (T(2)) is ∼0.8° to 1.5°C higher than that of the first (T(1)). This difference in temperature (ΔT = T(2) - T(1)) is defined as the SIH response. The SIH response is reduced by different classes of anxiolytics. The SIH test is simple and robust, it does not require training of animals, and test compound effects on motor behavior, feeding, and nociception do not affect test outcome. Furthermore, it is one of few anxiety tests that focuses on the physiological component of anxiety.
authors.affiliation: Utrecht Institute for Pharmaceutical Sciences and Rudolf Magnus Institute of Neuroscience, Utrecht, The Netherlands.
authors.forename: Lucianne
authors.initials: L
authors.lastname: Groenink
authors.forename: Christiaan
authors.initials: C
authors.lastname: Vinkers
authors.forename: Ruud
authors.initials: R
authors.lastname: van Oorschot
authors.forename: Berend
authors.initials: B
authors.lastname: Olivier
mesh_terms: D000822:Animal Husbandry; D000818:Animals; D014151:Anti-Anxiety Agents / Q000494:pharmacology*; D001007:Anxiety / Q000517:prevention & control* / Q000523:psychology; D003625:Data Collection / Q000379:methods; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005334:Fever / Q000523:psychology*; D006799:Housing, Animal; D008297:Male; D051379:Mice; D012934:Social Isolation / Q000523:psychology*; D013315:Stress, Psychological / Q000523:psychology*
chemical_list: D014151:Anti-Anxiety Agents