title: Deletion of the Hoc and Soc capsid proteins affects the surface and cellular uptake properties of bacteriophage T4 derived nanoparticles.
abstract: Recently the use of engineered viral scaffolds in biotechnology and medical applications has been increasing dramatically. T4 phage capsid derived nanoparticles (NPs) have potential advantages as sensors and in biotechnology. These applications require that the physical properties and cellular uptake of these NPs be understood. In this study we used a T4 deletion mutant to investigate the effects of removing both the Hoc and Soc proteins from the capsid surface on T4 tailless NPs. The surface charge, zeta potential, size, and cellular uptake efficiencies for both the T4 NP and T4ΔHocΔSoc NP mutant were measured and compared using dynamic light scattering and flow cytometry and significant differences were detected.
authors.affiliation: Center of for Bio/Molecular Science and Engineering, Naval Research Laboratory, 4555 Overlook Ave. SW, Washington, DC 20375, United States.
authors.forename: Kelly
authors.initials: K
authors.lastname: Robertson
authors.forename: Yoko
authors.initials: Y
authors.lastname: Furukawa
authors.forename: Alison
authors.initials: A
authors.lastname: Underwood
authors.forename: Lindsay
authors.initials: L
authors.lastname: Black
authors.forename: Jinny L
authors.initials: JL
authors.lastname: Liu
mesh_terms: D017122:Bacteriophage T4 / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001709:Biotechnology; D002213:Capsid / Q000737:chemistry*; D036022:Capsid Proteins / Q000235:genetics*; D005434:Flow Cytometry; D017353:Gene Deletion; D008027:Light; D053758:Nanoparticles / Q000737:chemistry*; D012542:Scattering, Radiation; D013499:Surface Properties
chemical_list: D036022:Capsid Proteins; C108525:HOC protein, T4 phage; C103392:small outer capsid protein, bacteriophage T4