title: Automatic flow-batch system for the sample treatment and determination of hydroxyproline in sausages.
abstract: In this study an automatic method for sample treatment and spectrophotometric determination of hydroxyproline in commercial sausages was developed. A flow-batch system that includes the steps of sample hydrolysis and determination of the analyte was designed. The method presents a linear range between 0.60 and 3.60 μg mL(-1) of hydroxyproline. A relative standard deviation of 1.68% was obtained from hydroxyproline standard solution (n=6, 1.20 μg mL(-1)) and the detection limit was 0.12 μg mL(-1). The sample throughput was 1 sample h(-1) while the reference method (AOAC) was carried out in about 17 h. This method employs 16 h of hydrolysis while in the proposed method the hydrolysis time was 15 min. For this purpose a pressure hydrolysis chamber with a low cost halogen lamp was used. The flow-batch system is simple and allows the use of both chambers (hydrolysis and detection) simultaneously. The obtained results with the flow-batch method are in good agreement with that obtained with the reference method. Moreover, it is good alternative to the quality control of meat products.
authors.affiliation: Department of Chemistry, Universidad Nacional del Sur, INQUISUR (UNS-CONICET), Av. Alem 1253 (B8000CPB), Bahía Blanca, Buenos Aires, Argentina.
authors.forename: J A
authors.initials: JA
authors.lastname: Belloni
authors.forename: M S
authors.initials: MS
authors.lastname: Di Nezio
authors.forename: M F
authors.initials: MF
authors.lastname: Pistonesi
authors.forename: M E
authors.initials: ME
authors.lastname: Centurión
mesh_terms: D000818:Animals; D057205:Automation, Laboratory / Q000295:instrumentation / Q000379:methods*; D002138:Calibration; D002417:Cattle; D006868:Hydrolysis; D006909:Hydroxyproline / Q000032:analysis*; D057230:Limit of Detection; D008461:Meat Products / Q000032:analysis*; D011312:Pressure; D011786:Quality Control; D012015:Reference Standards; D012996:Solutions; D013053:Spectrophotometry; D013552:Swine; D013997:Time Factors
chemical_list: D012996:Solutions; D006909:Hydroxyproline