title: Population-based study of DNA image cytometry as screening method for esophageal cancer.
abstract: OBJECTIVE
  To explore the DNA image cytometry (DNA-ICM) technique as a primary screening method for esophageal squamous precancerous lesions.
  
  
  METHODS
  This study was designed as a population-based screening study. A total of 582 local residents aged 40 years-69 years were recruited from Linzhou in Henan and Feicheng in Shandong. However, only 452 subjects had results of liquid-based cytology, DNA-ICM and pathology. The sensitivity and specificity of DNA-ICM were calculated and compared with liquid-based cytology in moderate dysplasia or worse.
  
  
  RESULTS
  Sensitivities of DNA-ICM ranging from at least 1 to 4 aneuploid cells were 90.91%, 86.36%, 79.55% and 77.27%, respectively, which were better than that of liquid-based cytology (75%). Specificities of DNA-ICM were 70.83%, 84.07%, 92.65% and 96.81%, but the specificity of liquid-based cytology was 91.91%. The sensitivity and specificity of a combination of liquid-based cytology and DNA-ICM were 84.09% and 85.78%, respectively.
  
  
  CONCLUSIONS
  It is possible to use DNA-ICM technique as a primary screening method for esophageal squamous precancerous lesions.
authors.affiliation: Department of Cancer Epidemiology, Cancer Institute/Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhao
authors.forename: Wen-Qiang
authors.initials: WQ
authors.lastname: Wei
authors.forename: De-Li
authors.initials: DL
authors.lastname: Zhao
authors.forename: Chang-Qing
authors.initials: CQ
authors.lastname: Hao
authors.forename: Dong-Mei
authors.initials: DM
authors.lastname: Lin
authors.forename: Qin-Jing
authors.initials: QJ
authors.lastname: Pan
authors.forename: Xin-Qing
authors.initials: XQ
authors.lastname: Li
authors.forename: Fu-Hua
authors.initials: FH
authors.lastname: Lei
authors.forename: Jin-Wu
authors.initials: JW
authors.lastname: Wang
authors.forename: Guo-Qing
authors.initials: GQ
authors.lastname: Wang
authors.forename: Qi
authors.initials: Q
authors.lastname: Shang
authors.forename: You-Lin
authors.initials: YL
authors.lastname: Qiao
mesh_terms: D000328:Adult; D000368:Aged; D002681:China; D003581:Cytodiagnosis / Q000379:methods; D004247:DNA; D004938:Esophageal Neoplasms / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology; D005260:Female; D006801:Humans; D019044:Image Cytometry / Q000379:methods*; D008297:Male; D008403:Mass Screening / Q000379:methods*; D008875:Middle Aged; D011230:Precancerous Conditions / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology; D012372:ROC Curve; D012680:Sensitivity and Specificity
chemical_list: D004247:DNA