title: Down-regulation of Pm4CL1 gene expression in E. coli model by antisense mRNA.
abstract: The coumarate:coenzyme A ligase 1 gene (4CL1) was isolated from secondary developing xylem tissues of Pinus massoniana Lamb (Pm4CL1). The Pm4CL1 gene was inserted into a pQE30 expression vector in both sense and antisense orientations to form a pQE30-4CL1-anti4CL1. The expression level of the Pm4CL1 gene in Escherichia coli cells was inhibited by the antisense mRNA of the Pm4CL1 gene. The results of reverse transcriptase polymerase chain reaction and sodium dodecyl sulfate polyacrylamide gel electrophoresis revealed that the average mRNA and protein expression levels of the Pm4CL1 gene in E. coli cells were decreased by 43 and 46%, respectively. These results are an important basis for further investigation.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China. hvhuanbiotech@gmail.com
authors.forename: Ha Van
authors.initials: HV
authors.lastname: Huan
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.forename: Siliang
authors.initials: S
authors.lastname: Zhang
mesh_terms: D003066:Coenzyme A Ligases / Q000235:genetics*; D015536:Down-Regulation / Q000235:genetics*; D004926:Escherichia coli / Q000166:cytology / Q000235:genetics*; D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D008031:Lignin / Q000096:biosynthesis; D028223:Pinus / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D016372:RNA, Antisense / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D016372:RNA, Antisense; D012333:RNA, Messenger; D008031:Lignin; D003066:Coenzyme A Ligases