title: Efficient growth suppression and apoptosis in human laryngeal carcinoma cell line HEP-2 induced by an adeno-associated virus expressing human FAS ligand.
abstract: BACKGROUND
  Apoptosis induced by Fas/FasL system has been proposed as a gene therapy methold for various cancers.
  
  
  METHODS
  We used adeno-associated virus-expressing enhanced green fluorescent protein (EGFP)-human FasL (AAV-EGFP-hFasL) to deliver FasL into Hep-2 cells, cytotoxicity was detected by MTS assay , apoptosis was confirmed by flow cytometry. We also treated the xenograft of Hep-2 tumor in nude mice with intratumoral injection of AAV-EGFP-hFasL. The size of the xenograft, the apoptosis in the xenograft, and the survival rate of the inoculated mice were then evaluated.
  
  
  RESULTS
  Hep-2 cells infected with AAV-EGFP-hFasL showed increased apoptosis rate and killing effect compared with AAV-EGFP-infected cells. In addition intratumoral injections of AAV-EGFP-hFasL into Hep-2 xenografts induced significant growth suppression of tumors.
  
  
  CONCLUSIONS
  Our findings suggest that the introduction of FasL into head and neck squamous cell carcinoma may induce significant apoptosis, and adeno-associated virus may be a useful vehicle for gene therapy.
authors.affiliation: Department of Otolaryngology-Head & Neck Surgery, Peking University First Hospital, Beijing, China.
authors.forename: Haili
authors.initials: H
authors.lastname: Sun
authors.forename: Yuhe
authors.initials: Y
authors.lastname: Liu
authors.forename: Dingfang
authors.initials: D
authors.lastname: Bu
authors.forename: Xiang
authors.initials: X
authors.lastname: Liu
authors.forename: James S
authors.initials: JS
authors.lastname: Norris
authors.forename: Shuifang
authors.initials: S
authors.lastname: Xiao
mesh_terms: D000818:Animals; D017209:Apoptosis; D045744:Cell Line, Tumor; D000229:Dependovirus; D053222:Fas Ligand Protein / Q000235:genetics*; D005434:Flow Cytometry; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000628:therapy*; D051379:Mice; D008819:Mice, Nude; D061848:Optical Imaging; D023041:Xenograft Model Antitumor Assays
chemical_list: D053222:Fas Ligand Protein