title: Coinfection of Leishmania chagasi with Toxoplasma gondii, Feline Immunodeficiency Virus (FIV) and Feline Leukemia Virus (FeLV) in cats from an endemic area of zoonotic visceral leishmaniasis.
abstract: The aim of the present study was to determine the coinfection of Leishmania sp. with Toxoplasma gondii, Feline Immunodeficiency Virus (FIV) and Feline Leukemia Virus (FeLV) in a population of cats from an endemic area for zoonotic visceral leishmaniasis. An overall 66/302 (21.85%) cats were found positive for Leishmania sp., with infection determined by direct parasitological examination in 30/302 (9.93%), by serology in 46/302 (15.23%) and by both in 10/302 (3.31%) cats. Real time PCR followed by amplicon sequencing successfully confirmed Leishmania infantum (syn Leishmania chagasi) infection. Out of the Leishmania infected cats, coinfection with FIV was observed in 12/66 (18.18%), with T. gondii in 17/66 (25.75%) and with both agents in 5/66 (7.58%) cats. FeLV was found only in a single adult cat with no Leishmania infection. A positive association was observed in coinfection of Leishmania and FIV (p<0.0001), but not with T. gondii (p>0.05). In conclusion, cats living in endemic areas of visceral leishmaniasis are significantly more likely to be coinfected with FIV, which may present confounding clinical signs and therefore cats in such areas should be always carefully screened for coinfections.
authors.affiliation: Department of Clinics, Surgery and Animal Reproduction, College of Veterinary Medicine, São Paulo State University, Araçatuba, São Paulo 16050-680, Brazil.
authors.forename: Ludmila Silva Vicente
authors.initials: LS
authors.lastname: Sobrinho
authors.forename: Cláudio Nazaretian
authors.initials: CN
authors.lastname: Rossi
authors.forename: Juliana Peloi
authors.initials: JP
authors.lastname: Vides
authors.forename: Eveline Tozzi
authors.initials: ET
authors.lastname: Braga
authors.forename: Ana Amélia Domingues
authors.initials: AA
authors.lastname: Gomes
authors.forename: Valéria Marçal Félix
authors.initials: VM
authors.lastname: de Lima
authors.forename: Sílvia Helena Venturoli
authors.initials: SH
authors.lastname: Perri
authors.forename: Diego
authors.initials: D
authors.lastname: Generoso
authors.forename: Hélio
authors.initials: H
authors.lastname: Langoni
authors.forename: Christian
authors.initials: C
authors.lastname: Leutenegger
authors.forename: Alexander Welker
authors.initials: AW
authors.lastname: Biondo
authors.forename: Márcia Dalastra
authors.initials: MD
authors.lastname: Laurenti
authors.forename: Mary
authors.initials: M
authors.lastname: Marcondes
mesh_terms: D000818:Animals; D001938:Brazil / Q000453:epidemiology; D002415:Cats; D060085:Coinfection / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary / Q000821:virology; D019353:Endemic Diseases / Q000662:veterinary; D005260:Female; D006801:Humans; D016089:Immunodeficiency Virus, Feline; D007898:Leishmaniasis, Visceral / Q000150:complications / Q000453:epidemiology / Q000662:veterinary*; D016180:Lentivirus Infections / Q000150:complications / Q000453:epidemiology / Q000662:veterinary*; D005256:Leukemia Virus, Feline; D016582:Leukemia, Feline / Q000150:complications* / Q000453:epidemiology; D008297:Male; D014124:Toxoplasmosis, Animal / Q000150:complications* / Q000453:epidemiology; D015047:Zoonoses