title: Roles of the coding and noncoding regions of rift valley Fever virus RNA genome segments in viral RNA packaging.
abstract: We characterized the RNA elements involved in the packaging of Rift Valley fever virus RNA genome segments, L, M, and S. The 5'-terminal 25 nucleotides of each RNA segment were equally competent for RNA packaging and carried an RNA packaging signal, which overlapped with the RNA replication signal. Only the deletion mutants of L RNA, but not full-length L RNA, were efficiently packaged, implying the possible requirement of RNA compaction for L RNA packaging.
authors.affiliation: Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, Texas, USA.
authors.forename: Shin
authors.initials: S
authors.lastname: Murakami
authors.forename: Kaori
authors.initials: K
authors.lastname: Terasaki
authors.forename: Krishna
authors.initials: K
authors.lastname: Narayanan
authors.forename: Shinji
authors.initials: S
authors.lastname: Makino
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D016679:Genome, Viral; D006801:Humans; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012367:RNA, Viral / Q000235:genetics*; D012295:Rift Valley Fever / Q000821:virology*; D012296:Rift Valley fever virus / Q000235:genetics* / Q000502:physiology; D019065:Virus Assembly; D014779:Virus Replication
chemical_list: D012367:RNA, Viral