title: Site-directed mutagenesis in bacteriorhodopsin mutants and their characterization for bioelectrical and biotechnological equipment.
abstract: Bacteriorhodopsin (BR) mutagenesis plays an important role in the development of BR-based materials and tools with enhanced optical and electrical properties. Previously reported protocols for generating BR mutations are inefficient for the preparation and purification of mutant proteins. Therefore, a series of BR mutations were generated by using improved methods, which are described in further detail. The functional activity of the recombinant proteins was confirmed by spectroscopic and electrochemical assays. Modified proteins with different wavelengths and activities form a foundation for color-sensitive sensors and can be utilized to produce unique bioelectrical and biotechnological tools and materials. The proton-pumping activity of the generated mutant D85E was normal, indicating that the mutant could be used in light batteries. However, mutants D85Q and D85N were almost inactive; and D85N had a prolonged M state, suggesting that it could be utilized in light memories.
authors.affiliation: Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran. saeedi.p@gmail.com
authors.forename: Pardis
authors.initials: P
authors.lastname: Saeedi
authors.forename: Jafar Mohammadian
authors.initials: JM
authors.lastname: Moosaabadi
authors.forename: Seyed Sina
authors.initials: SS
authors.lastname: Sebtahmadi
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Behmanesh
authors.forename: Jalil Fallah
authors.initials: JF
authors.lastname: Mehrabadi
mesh_terms: D019943:Amino Acid Substitution; D001436:Bacteriorhodopsins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D001674:Bioelectric Energy Sources; D001709:Biotechnology / Q000379:methods*; D004252:DNA Mutational Analysis; D055664:Electrochemical Techniques; D016297:Mutagenesis, Site-Directed; D013057:Spectrum Analysis
chemical_list: D001436:Bacteriorhodopsins