title: The influence of fluoride on the expression of inhibitors of Wnt/β-catenin signaling pathway in rat skin fibroblast Cells.
abstract: The effective therapy of fluoride-induced bone diseases requires an understanding of the mechanism of the disorders. Changes in the inhibitors of the Wnt/β-catenin pathway, Dickkopf-1 (Dkk-1) and Sclerostin (SOST),were studied in supernatants harvested from rat skin fibroblasts cultured with varied doses of fluoride. The contents of SOST and Dkk-1 in fibroblast supernatants were assessed at four exposure time-points and investigated by using the method of ELISA. Compared to the relevant controls(0 mg F(−)/L), a significant decrease of the concentrations of SOST and Dkk-1 was observed as the fluoride concentration increased. Compared to the relevant time controls (24 h), a significant decrease of the concentrations of SOST and Dkk-1 was observed with the extension of time. Our results suggest that the Wnt/β-catenin pathway inhibitors Dkk-1 and SOST play an important role in skeletal fluorosis. They can be used as important indications for diagnosing bone metabolism changes caused by fluoride exposure and therapeutic targets in diseases resulting from fluoride exposure.
authors.affiliation: Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Hubei Wuhan 430030, People’s Republic of China.
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Liu
authors.forename: Chang-Cheng
authors.initials: CC
authors.lastname: Li
authors.forename: Ke-Jian
authors.initials: KJ
authors.lastname: Liu
authors.forename: Cai-Yan
authors.initials: CY
authors.lastname: Cui
authors.forename: Yu-Zeng
authors.initials: YZ
authors.lastname: Zhang
authors.forename: Yun
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000378:metabolism; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D005459:Fluorides / Q000494:pharmacology*; D005819:Genetic Markers; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D051381:Rats; D012867:Skin / Q000166:cytology / Q000378:metabolism*; D060449:Wnt Signaling Pathway / Q000187:drug effects*
chemical_list: D019485:Bone Morphogenetic Proteins; C531384:Dkk1 protein, rat; D005819:Genetic Markers; D036341:Intercellular Signaling Peptides and Proteins; C486031:Sost protein, rat; D005459:Fluorides