title: Prediction of dynamic metabolic behavior of Pediococcus pentosaceus producing lactic acid from lignocellulosic sugars.
abstract: A dynamic metabolic model is presented for Pediococcus pentosaceus producing lactic acid from lignocellulose-derived mixed sugars including glucose, mannose, galactose, arabinose, and xylose. Depending on the pairs of mixed sugars, P. pentosaceus exhibits diverse (i.e., sequential, simultaneous or mixed) consumption patterns. This regulatory behavior of P. pentosaceus is portrayed using the hybrid cybernetic model (HCM) framework which views elementary modes of the network as metabolic options dynamically modulated. Comprehensive data are collected for model identification and validation through fermentation experiments involving single substrates and various combinations of mixed sugars. Most sugars are metabolized rather sequentially while co-consumption of galactose and arabinose is observed. It is demonstrated that the developed HCM successfully predicts mixed sugar data based on the parameters identified mostly from single substrate data only. Further, we discuss the potential of HCMs as a tool for predicting intracellular flux distribution with comparison with flux balance analysis.
authors.affiliation: Institute of Nutrition and Food Sciences, Division of Food Technology and Biotechnology, University of Bonn, D-53117 Bonn, Germany. philipp.adler@uni-bonn.de
authors.forename: Philipp
authors.initials: P
authors.lastname: Adler
authors.forename: Hyun-Seob
authors.initials: HS
authors.lastname: Song
authors.forename: Katharina
authors.initials: K
authors.lastname: Kästner
authors.forename: Doraiswami
authors.initials: D
authors.lastname: Ramkrishna
authors.forename: Benno
authors.initials: B
authors.lastname: Kunz
mesh_terms: D050260:Carbohydrate Metabolism; D019344:Lactic Acid / Q000096:biosynthesis*; D008031:Lignin / Q000378:metabolism*; D008954:Models, Biological; D010376:Pediococcus / Q000378:metabolism*
chemical_list: C036909:lignocellulose; D019344:Lactic Acid; D008031:Lignin