title: Verbascoside, isoverbascoside, and their derivatives recovered from olive mill wastewater as possible food antioxidants.
abstract: Olive oil processing industries generate substantial quantities of phenolic-rich byproducts, which could be valuable natural sources of antioxidants. This work is focused on the recovery and structural characterization of antioxidant compounds from olive mill wastewater (OMWW), a polluting byproduct of the olive oil production process. Phenolics were extracted from the waste material using a membrane technology coupled to low-pressure gel filtration chromatography on a Sephadex LH-20. The LH-20 fraction was, in turn, characterized for its phenolic composition by HPLC-DAD-MS/MS analyses. Verbascoside, isoverbascoside, β-hydroxyverbascoside, β-hydroxyisoverbascoside, and various oxidized phenolics were identified. Uptake of verbascoside, purified from the LH-20 fraction, by HT-29 cells, an established model system for studying drug transport properties, was also assayed. Finally, the antioxidant activities of the LH-20 fraction and verbascoside were characterized by two different techniques. Individual verbascoside was more active as a scavenger of reactive oxygen species and as a chemopreventive agent protecting low-density lipoproteins from oxidative damage than the LH-20 fraction.
authors.affiliation: Institute of Sciences of Food Production, CNR, Bari, Italy.
authors.forename: Angela
authors.initials: A
authors.lastname: Cardinali
authors.forename: Sandra
authors.initials: S
authors.lastname: Pati
authors.forename: Fiorenza
authors.initials: F
authors.lastname: Minervini
authors.forename: Isabella
authors.initials: I
authors.lastname: D'Antuono
authors.forename: Vito
authors.initials: V
authors.lastname: Linsalata
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Lattanzio
mesh_terms: D000975:Antioxidants / Q000302:isolation & purification*; D005503:Food Additives; D005511:Food Handling / Q000379:methods; D005960:Glucosides / Q000302:isolation & purification* / Q000378:metabolism; D019073:HT29 Cells; D006801:Humans; D007220:Industrial Waste / Q000032:analysis*; D031658:Olea / Q000737:chemistry*; D000069463:Olive Oil; D010636:Phenols / Q000302:isolation & purification* / Q000378:metabolism; D010938:Plant Oils; D059027:Recycling; D059808:Polyphenols
chemical_list: D000975:Antioxidants; D005503:Food Additives; D005960:Glucosides; D007220:Industrial Waste; D000069463:Olive Oil; D010636:Phenols; D010938:Plant Oils; C058956:acteoside; D059808:Polyphenols; C064683:isoacteoside