title: Novel Pyridinium compound from marine actinomycete, Amycolatopsis alba var. nov. DVR D4 showing antimicrobial and cytotoxic activities in vitro.
abstract: Marine sediment samples from Visakhapatnam coast of Bay of Bengal, India, were investigated as a source of actinomycetes to screen for the production of antibiotics and cytotoxic compounds. Actinomycete strain DVR D4 with interesting bioactivity profile was isolated during our systematic study of marine actinomycetes. Based on biochemical properties and 16S rDNA analysis the isolate DVR D4 was identified as a strain of Amycolatopsis alba. A solvent extraction followed by a chromatographic purification helped to isolate a cytotoxic compound, which was identified as 1(10-aminodecyl) Pyridinium salt antibiotic, on the basis of spectral data. The compound showed potent cytotoxic activity against cancer cell lines of cervix (HeLa), breast (MCF-7) and brain (U87MG) in vitro and also exhibited antibacterial activities against Gram-positive and Gram-negative bacteria.
authors.affiliation: Center for Biotechnology, College of Engineering, Andhra University, Visakhapatnam 530 003, Andhra Pradesh, India. ravidvr@gmail.com
authors.forename: Venkata Ratna Ravi Kumar
authors.initials: VR
authors.lastname: Dasari
authors.forename: Murali Krishna Kumar
authors.initials: MK
authors.lastname: Muthyala
authors.forename: Murali Yugandhar
authors.initials: MY
authors.lastname: Nikku
authors.forename: Sri Rami Reddy
authors.initials: SR
authors.lastname: Donthireddy
mesh_terms: D000192:Actinomycetales / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000378:metabolism*; D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000378:metabolism*; D001419:Bacteria / Q000187:drug effects; D045744:Cell Line, Tumor; D002845:Chromatography; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D019015:Geologic Sediments / Q000382:microbiology; D006801:Humans; D007194:India; D009052:Leukemia Virus, Murine; D011726:Pyridinium Compounds / Q000302:isolation & purification / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D011726:Pyridinium Compounds; D012336:RNA, Ribosomal, 16S