title: Extract of Lillium candidum L. can modulate the genotoxicity of the antibiotic zeocin.
abstract: Lilium candidum L. extract (LE) is well known in folk medicine for the treatment of burns, ulcers, inflammations and for healing wounds. This work aims to clarify whether the genotoxic potential of the radiomimetic antibiotic zeocin (Zeo) could be modulated by LE. Our results indicate that LE exerts no cytotoxic, DNA-damaging and clastogenic activity in in Chlamydomonas reinhardtii, Pisum sativum L. and Hordeum vulgare L. test systems over a broad concentration range. Weak but statistically significant clastogenic effects due to the induction of micronuclei and chromosome aberrations have been observed in H. vulgare L. after treatment with 200 and 300 μg/mL LE. To discriminate protective from adverse action of LE different experimental designs have been used. Our results demonstrate that the treatment with mixtures of LE and Zeo causes an increase in the level of DNA damage, micronuclei and "metaphases with chromatid aberrations" (MwA). Clear evidence has been also obtained indicating that pretreatment with LE given 4 h before the treatment with Zeo accelerates the rejoining kinetics of Zeo-induced DNA damage in P. sativum L. and C. reinhardtii, and can decrease clastogenic effect of Zeo measured as frequencies of micronuclei and MwA in H. vulgare L. Here, we show for the first time that LE can modulate the genotoxic effects of zeocin. The molecular mode of action strongly depends on the experimental design and varies from synergistic to protective effect (adaptive response-AR). Our results also revealed that LE-induced AR to zeocin involves up-regulation of DSB rejoining in C. reinhardtii and P. sativum L. cells.
authors.affiliation: Department of Genetics, Faculty of Natural Sciences, Comenius University, Mlynska dolina B-1, Bratislava 842 15, Slovakia.
authors.forename: Marcela
authors.initials: M
authors.lastname: Kopaskova
authors.forename: Lina
authors.initials: L
authors.lastname: Hadjo
authors.forename: Bisera
authors.initials: B
authors.lastname: Yankulova
authors.forename: Gabriele
authors.initials: G
authors.lastname: Jovtchev
authors.forename: Eliska
authors.initials: E
authors.lastname: Galova
authors.forename: Andrea
authors.initials: A
authors.lastname: Sevcovicova
authors.forename: Pavel
authors.initials: P
authors.lastname: Mucaji
authors.forename: Eva
authors.initials: E
authors.lastname: Miadokova
authors.forename: Peter
authors.initials: P
authors.lastname: Bryant
authors.forename: Stephka
authors.initials: S
authors.lastname: Chankova
mesh_terms: D000900:Anti-Bacterial Agents / Q000633:toxicity*; D001761:Bleomycin / Q000633:toxicity*; D016825:Chlamydomonas reinhardtii / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects; D001467:Hordeum / Q000187:drug effects; D027762:Lilium / Q000737:chemistry*; D018519:Meristem / Q000187:drug effects; D008940:Mitotic Index; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D018532:Pisum sativum / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D009153:Mutagens; D010936:Plant Extracts; D001761:Bleomycin; C105427:Zeocin