title: Acetone-butanol fermentation of marine macroalgae.
abstract: The objective of this study was to subject mannitol, either as a sole carbon source or in combination with glucose, and aqueous extracts of the kelp Saccharina spp., containing mannitol and laminarin, to acetone-butanol fermentation by Clostridium acetobutylicum (ATCC 824). Both mannitol and glucose were readily fermented. Mixed substrate fermentations with glucose and mannitol resulted in diauxic growth of C. acetobutylicum with glucose depletion preceding mannitol utilization. Fermentation of kelp extract exhibited triauxic growth, with an order of utilization of free glucose, mannitol, and bound glucose, presumably laminarin. The lag in laminarin utilization reflected the need for enzymatic hydrolysis of this polysaccharide into fermentable sugars. The butanol and total solvent yields were 0.12 g/g and 0.16 g/g, respectively, indicating that significant improvements are still needed to make industrial-scale acetone-butanol fermentations of seaweed economically feasible.
authors.affiliation: Pacific Northwest National Laboratory, Marine Sciences Laboratory, 1529 West Sequim Bay Road, Sequim, WA 98382, USA. michael.huesemann@pnl.gov
authors.forename: Michael H
authors.initials: MH
authors.lastname: Huesemann
authors.forename: Li-Jung
authors.initials: LJ
authors.lastname: Kuo
authors.forename: Lindsay
authors.initials: L
authors.lastname: Urquhart
authors.forename: Gary A
authors.initials: GA
authors.lastname: Gill
authors.forename: Guri
authors.initials: G
authors.lastname: Roesijadi
mesh_terms: D000096:Acetone / Q000378:metabolism; D000440:Butanols / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D046969:Clostridium acetobutylicum / Q000254:growth & development / Q000378:metabolism*; D045424:Complex Mixtures / Q000737:chemistry*; D005285:Fermentation; D005936:Glucans; D005947:Glucose / Q000032:analysis / Q000378:metabolism*; D007218:Industrial Microbiology / Q000191:economics / Q000379:methods*; D008353:Mannitol / Q000032:analysis / Q000378:metabolism*; D000459:Phaeophyceae / Q000737:chemistry*; D011134:Polysaccharides / Q000032:analysis / Q000378:metabolism*
chemical_list: D000440:Butanols; D045424:Complex Mixtures; D005936:Glucans; D011134:Polysaccharides; D000096:Acetone; D008353:Mannitol; C008247:laminaran; D005947:Glucose