title: Detection of non-amplified Mycobacterium tuberculosis genomic DNA using piezoelectric DNA-based biosensors.
abstract: Piezoelectric DNA-based biosensor technology was developed as a new method for detection of M. tuberculosis. This method consists of immobilizing a thiol-modified oligonucleotide probe on the gold electrode surface of a quartz crystal, using a self-assembled monolayer method. The advantage of this study is that a non-amplified genomic bacterial DNA target was used. Instead, the genomic DNA was digested by restriction enzyme to obtain DNA fragments containing the target sequence. The fabricated biosensor was evaluated through an examination of 200 samples. No cross hybridization were observed against M. avium complex and other microorganisms. This target DNA preparation, without PCR amplification, will reduce time, costs, and the tedious step of amplification.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University, Sukhumvit 23, Bangkok 10110, Thailand. tkaewphinit@yahoo.com
authors.forename: Thongchai
authors.initials: T
authors.lastname: Kaewphinit
authors.forename: Somchai
authors.initials: S
authors.lastname: Santiwatanakul
authors.forename: Chamras
authors.initials: C
authors.lastname: Promptmas
authors.forename: Kosum
authors.initials: K
authors.lastname: Chansiri
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D055664:Electrochemical Techniques / Q000379:methods; D004566:Electrodes; D006046:Gold; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000302:isolation & purification*; D015345:Oligonucleotide Probes / Q000737:chemistry / Q000378:metabolism; D013438:Sulfhydryl Compounds
chemical_list: D004269:DNA, Bacterial; D015345:Oligonucleotide Probes; D013438:Sulfhydryl Compounds; D006046:Gold