title: Tannase production by Penicillium purpurogenum PAF6 in solid state fermentation of tannin-rich plant residues following OVAT and RSM.
abstract: Tannase production by newly isolated Penicillium purpurogenum PAF6 was investigated by 'one variable at a time' (OVAT) approach followed by response surface methodology (RSM). Tannin-rich plant residues were used as supporting solid substrate and sole carbon source and, among them, tamarind seed was found to be the most favorable substrate than haritaki, pomegranate, tea leaf waste and arjun fruit. Physicochemical parameters were initially optimized using OVAT methodology and some important factors like incubation time, incubation temperature, substrate:moisture ratio as well as carbon, nitrogen and phosphate concentrations were verified with Box-Behken design of response surface methodology. Phosphate source, nitrogen source and temperature were found as the most favorable variables in the maximization of production. Tannase production was enhanced from 1.536 U/g to 5.784 U/g using tamarind seed OVAT optimization and further enhancement up to 6.15 U/g following RSM. An overall 3.76- and 4.0-fold increases in tannase production were achieved in OVAT and RSM, respectively.
authors.affiliation: Department of Microbiology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Arijit
authors.initials: A
authors.lastname: Jana
authors.forename: Chiranjit
authors.initials: C
authors.lastname: Maity
authors.forename: Suman Kumar
authors.initials: SK
authors.lastname: Halder
authors.forename: Keshab Chandra
authors.initials: KC
authors.lastname: Mondal
authors.forename: Bikash Ranjan
authors.initials: BR
authors.lastname: Pati
authors.forename: Pradeep Kumar Das
authors.initials: PK
authors.lastname: Mohapatra
mesh_terms: D002265:Carboxylic Ester Hydrolases / Q000096:biosynthesis*; D005110:Extracellular Space / Q000378:metabolism; D005285:Fermentation; D015233:Models, Statistical; D010407:Penicillium / Q000166:cytology / Q000302:isolation & purification / Q000378:metabolism*; D010936:Plant Extracts / Q000378:metabolism*; D012984:Software; D013634:Tannins / Q000378:metabolism*
chemical_list: D010936:Plant Extracts; D013634:Tannins; D002265:Carboxylic Ester Hydrolases; C022711:tannase