title: Validation of a microscale extraction and high-throughput UHPLC-QTOF-MS analysis method for huperzine A in Huperzia.
abstract: Herein we report on an improved method for the microscale extraction of huperzine A (HupA), an acetylcholinesterase-inhibiting alkaloid, from as little as 3 mg of tissue homogenate from the clubmoss Huperzia squarrosa (G. Forst.) Trevis with 99.95% recovery. We also validated a novel UHPLC-QTOF-MS method for the high-throughput analysis of H. squarrosa extracts in only 6 min, which, in combination with the very low limit of detection (20 pg on column) and the wide linear range for quantification (20-10,000 pg on column), allow for a highly efficient screening of extracts containing varying amounts of HupA. Utilization of this methodology has the potential to conserve valuable plant resources.
authors.affiliation: Institute of Biological Chemistry and M.J. Murdock Metabolomics Laboratory, Washington State University, Pullman, WA 99164-6340, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Cuthbertson
authors.forename: Jasenka
authors.initials: J
authors.lastname: Piljac-Žegarac
authors.forename: Bernd Markus
authors.initials: BM
authors.lastname: Lange
mesh_terms: D000470:Alkaloids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D057166:High-Throughput Screening Assays; D032501:Huperzia / Q000737:chemistry*; D057230:Limit of Detection; D016014:Linear Models; D010936:Plant Extracts / Q000737:chemistry; D015203:Reproducibility of Results; D012717:Sesquiterpenes / Q000032:analysis*
chemical_list: D000470:Alkaloids; D010936:Plant Extracts; D012717:Sesquiterpenes; C050426:huperzine A