title: Molecular identification of Mycobacterium bovis and the importance of zoonotic tuberculosis in Mexican patients.
abstract: METHODS
  The epidemiology of zoonotic tuberculosis (ZTB) in humans in Mexico is poorly known.
  
  
  OBJECTIVE
  To identify isolates of Mycobacterium bovis in humans and cattle by polymerase chain reaction (PCR), and establish the clinical and epidemiological importance of ZTB in humans.
  
  
  METHODS
  From 1995 to 2009, 124 isolates from patients with TB and 60 isolates from cattle were analysed. PCR identification was performed using the oxy R gene, and the clinical and epidemiological aspects of ZTB in humans were investigated.
  
  
  RESULTS
  PCR identified 93 M. bovis isolates: 35 (28%) from the 124 human isolates and 58 (97%) from the 60 cattle isolates. The sensitivity and specificity of the method were 100%. ZTB in the 35 patients presented as extra-pulmonary TB (EPTB) in 74%: 51% were children, 69% had malnutrition, 51% had consumed unpasteurised milk and 6% had contact with animals; 11% were relapses and 31% died.
  
  
  CONCLUSIONS
  PCR using the oxy R gene is highly sensitive, specific and rapid for the identification of M. bois. ZTB is a serious public health problem, and presented as EPTB in children with malnutrition and those who had consumed unpasteurised milk. ZTB provokes relapses and a high mortality rate.
authors.affiliation: Laboratorio de Microbiología y Parasitología, Investigación en Microbiología Médica, Departamento de Microbiología y Patología, Centro Universitario de Ciencias de la Salud, Universidad  de Guadalajara, Guadalajara, Jalisco, México.
authors.forename: L
authors.initials: L
authors.lastname: Portillo-Gómez
authors.forename: E G
authors.initials: EG
authors.lastname: Sosa-Iglesias
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D002417:Cattle; D016009:Chi-Square Distribution; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D044342:Malnutrition / Q000401:mortality; D008800:Mexico / Q000453:epidemiology; D008875:Middle Aged; D008892:Milk / Q000382:microbiology; D017720:Molecular Epidemiology; D009163:Mycobacterium bovis / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D014380:Tuberculosis, Bovine / Q000175:diagnosis / Q000382:microbiology* / Q000401:mortality / Q000635:transmission; D015047:Zoonoses / Q000382:microbiology* / Q000635:transmission
chemical_list: D001426:Bacterial Proteins