title: Effects of prostaglandin E1 on the preparation of platelet concentrates in dogs.
abstract: BACKGROUND
  Platelet concentrates (PC) are prepared by centrifugation of platelet-rich plasma (PRP) that is prepared by centrifugation of whole blood. The resuspension of the platelet pellet during PC preparation from dogs is difficult because of platelet activation induced by centrifugation.
  
  
  OBJECTIVE
  To investigate the efficacy of adding prostaglandin E(1) (PGE(1) ) to prevent platelet activation during PC preparation from dogs.
  
  
  METHODS
  Fifteen healthy Beagle dogs.
  
  
  METHODS
  Prospective, experimental trial: PGE(1) was added to PRP before the high-speed centrifugation during PC preparation. To estimate the effect of this addition, we assessed the platelet aggregability before transfusion, the survival of the platelets after transfusion, and the platelet reactivity after transfusion, which is estimated by the P-selectin expression of the platelets when stimulated by thrombin.
  
  
  RESULTS
  The difficulty associated with platelet resuspension was resolved by PGE(1.) PGE(1) strongly inhibited platelet aggregation induced by collagen and ADP; however, it recovered after the platelets were resuspended in plasma without PGE(1) (mean aggregation ratio; collagen: 10.00-80.80%, ADP: 8.20-53.60%). Survival of the platelets after transfusion was not affected by PGE(1) (mean 8.04 and 7.56 days, without and with PGE(1) ), and thrombin-induced P-selectin expression after transfusion in PGE(1) -treated PC was also well maintained (mean positive ratio 53.7 and 47.9%, before and 24 hours after transfusion).
  
  
  CONCLUSIONS
  The addition of PGE(1) in PRP before the centrifugation of PRP can improve the preparation efficiency of PC from dogs, while maintaining the therapeutic efficacy of the platelets.
authors.affiliation: The Laboratory of Internal Medicine II, School of Veterinary Medicine, Azabu University, Kanagawa, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Segawa
authors.forename: T
authors.initials: T
authors.lastname: Kondo
authors.forename: S
authors.initials: S
authors.lastname: Kimura
authors.forename: A
authors.initials: A
authors.lastname: Fujimoto
authors.forename: T
authors.initials: T
authors.lastname: Kato
authors.forename: T
authors.initials: T
authors.lastname: Ishikawa
authors.forename: S
authors.initials: S
authors.lastname: Neo
authors.forename: M
authors.initials: M
authors.lastname: Hisasue
authors.forename: T
authors.initials: T
authors.lastname: Yamada
authors.forename: R
authors.initials: R
authors.lastname: Tsuchiya
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002498:Centrifugation / Q000662:veterinary; D015232:Dinoprostone / Q000494:pharmacology*; D004285:Dogs; D005434:Flow Cytometry / Q000662:veterinary; D015539:Platelet Activation / Q000187:drug effects*; D010974:Platelet Aggregation / Q000187:drug effects; D017713:Platelet Transfusion / Q000379:methods* / Q000662:veterinary*; D053657:Platelet-Rich Plasma / Q000166:cytology / Q000187:drug effects*; D011446:Prospective Studies
chemical_list: D015232:Dinoprostone