title: Model system for cell adhesion mediated by weak carbohydrate-carbohydrate interactions.
abstract: The multivalent carbohydrate-carbohydrate interaction between membrane-anchored epitopes derived from the marine sponge Microciona prolifera has been explored by colloidal probe microscopy. An in situ coupling of sulfated and non-sulfated disaccharides to membrane-coated surfaces was employed to mimic native cell-cell contacts.The dynamic strength of the homomeric self-association was measured as a function of calcium ions and loading rate. A deterministic model was used to estimate the basic energy landscape and number of participating bonds in the contact zone.
authors.affiliation: Institute of Physical Chemistry, University of Göttingen, Tammannstrasse 6, 37077 Göttingen, Germany.
authors.forename: Bärbel
authors.initials: B
authors.lastname: Lorenz
authors.forename: Luis
authors.initials: L
authors.lastname: Álvarez de Cienfuegos
authors.forename: Marieelen
authors.initials: M
authors.lastname: Oelkers
authors.forename: Ella
authors.initials: E
authors.lastname: Kriemen
authors.forename: Christian
authors.initials: C
authors.lastname: Brand
authors.forename: Milena
authors.initials: M
authors.lastname: Stephan
authors.forename: Eva
authors.initials: E
authors.lastname: Sunnick
authors.forename: Deniz
authors.initials: D
authors.lastname: Yüksel
authors.forename: Venkateshwarlu
authors.initials: V
authors.lastname: Kalsani
authors.forename: Krishna
authors.initials: K
authors.lastname: Kumar
authors.forename: Daniel B
authors.initials: DB
authors.lastname: Werz
authors.forename: Andreas
authors.initials: A
authors.lastname: Janshoff
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D004187:Disaccharides / Q000737:chemistry* / Q000302:isolation & purification; D008051:Lipid Bilayers / Q000737:chemistry*; D020527:Microscopy, Scanning Probe; D011161:Porifera / Q000737:chemistry*
chemical_list: D004187:Disaccharides; D008051:Lipid Bilayers