title: [Effect of Pulsatilla Decoction on the expression of proinflammatory cytokines in inflammatory bowel disease].
abstract: OBJECTIVE
  To investigate the molecular mechanisms underlying in the treatment of inflammatory bowel disease by Pulsatilla Decoction.
  
  
  METHODS
  Forty Wistar male rats were randomly divided into 5 groups( n = 8)control group, model group, model + positive control group (mesalazine), Pulsatilla Decoction treatment group, in addition, the Pulsatilla Decoction treatment group was divided into middle and high dose group. Intragastric administration was used in the positive control group and Pulsatilla Decoction treatment group. The expression of interleukin-1beta (IL-1beta), interleukin-6(IL-6) and tumor necrosis factor-alpha (TNF-alpha) were detected by real time PCR after extraction of RNA from colons.
  
  
  RESULTS
  Compared with the model group, positive medicine and Pulsatilla Decoction group, especially high-dose group, could effectively inhibit the expression of IL-1beta, IL-6 and TNF-alpha.
  
  
  CONCLUSIONS
  Pulsatilla Decoction could exert its effect in the treatment of inflammatory bowel disease by inhibiting the expression of proinflammatory cytokines.
authors.affiliation: Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Yu
authors.forename: Hong-Ju
authors.initials: HJ
authors.lastname: Liu
authors.forename: Huan-Huan
authors.initials: HH
authors.lastname: Dun
authors.forename: Qian
authors.initials: Q
authors.lastname: Dong
authors.forename: Chao
authors.initials: C
authors.lastname: Liang
mesh_terms: D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D015212:Inflammatory Bowel Diseases / Q000188:drug therapy* / Q000378:metabolism; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism*; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008517:Phytotherapy; D031946:Pulsatilla / Q000737:chemistry*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*
chemical_list: D004365:Drugs, Chinese Herbal; D053583:Interleukin-1beta; D015850:Interleukin-6; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha