title: Single-molecule atomic force microscopy on live cells compares aptamer and antibody rupture forces.
abstract: Some researchers have questioned whether synthetic aptamers bind as robustly as natural antibodies. To address this issue, we used single-molecule atomic force microscopy to measure the rupture force between a protein and both its aptamer and its antibody. The rupture force on live cell membranes between the aptamer and protein was 46 ± 26 pN; the force with the antibody was 68 ± 33 pN, we conclude that the binding forces are about equal.
authors.affiliation: Department of Chemistry, Center for Research at the Bio/Nano Interface, UF Genetics Institute, University of Florida, Gainesville, FL 32611-7200, USA.
authors.forename: Meghan B
authors.initials: MB
authors.lastname: O'Donoghue
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Shi
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Fang
authors.forename: Weihong
authors.initials: W
authors.lastname: Tan
mesh_terms: D000906:Antibodies / Q000737:chemistry* / Q000378:metabolism; D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000378:metabolism; D002477:Cells / Q000737:chemistry* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D018625:Microscopy, Atomic Force; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D000906:Antibodies; D052157:Aptamers, Nucleotide; D011506:Proteins