title: Identification of genes preferentially expressed in goat hair follicle anagen-catagen transition using suppression subtractive hybridization.
abstract: Suppressive subtraction hybridization (SSH) was used to identify differentially expressed genes in goat (Capra hircus) hair follicle anagen-catagen transition. The cDNA fragments, derived from SSH positive subtractive library (tester: anagen-catagen transition, driver: later anagen), were cloned into pEGM-T vector. Two hundred cDNA fragments screened from this library were subjected to identify forty-five unregulated isolates. Sequence analysis revealed that these fragments represented twenty-three genes. Blasting analysis with database in GenBank showed that twenty genes were previously clearly annotated, two were homologous to un-annotated expressed sequence tag (ESTs), and one might be novel. To identify characters of gene expression, seven genes in later anagen and anagen-catagen transition skin tissues were chosen for quantitative real-time PCR. Results indicated that expression of these seven genes varied much, reaching threefold among them, furthering indicating that expression of those genes was up-regulation in the anagen-catagen transition. We characterized expression levels of this potential novel gene and the goat ectodysplasin A during differential stages of hair cycle. These profiles suggested that these two genes might play a role in the goat secondary hair follicle cycle.
authors.affiliation: College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi, China.
authors.forename: W
authors.initials: W
authors.lastname: Jiang
authors.forename: Y X
authors.initials: YX
authors.lastname: Yang
authors.forename: P
authors.initials: P
authors.lastname: Xue
authors.forename: Y J
authors.initials: YJ
authors.lastname: Huang
authors.forename: Y L
authors.initials: YL
authors.lastname: Chen
mesh_terms: D000822:Animal Husbandry; D000818:Animals; D019295:Computational Biology; D018076:DNA, Complementary / Q000032:analysis* / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods; D006041:Goats / Q000235:genetics* / Q000378:metabolism; D018859:Hair Follicle / Q000378:metabolism / Q000502:physiology*; D007381:Intermediate Filament Proteins / Q000235:genetics / Q000378:metabolism; D009693:Nucleic Acid Hybridization / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction; D016415:Sequence Alignment
chemical_list: D018076:DNA, Complementary; D007381:Intermediate Filament Proteins