title: Identification of miR414 and expression analysis of conserved miRNAs from Stevia rebaudiana.
abstract: MicroRNAs (miRNAs) usually contain 19-24 nucleotides and have been identified as important eukaryotic gene regulators. Applications of various computational approaches have simplified the task by predicting miRNAs from available sequence data sources. In this study, we identified a conserved miR414 from a computational analysis of EST sequence data available from Stevia rebaudiana. In addition, we also identified six conserved miRNAs namely miR169, miR319, miR414, miR164, miR167 and miR398 using stem-loop RT-PCR analysis. Hence, miR414 was commonly identified using both methods. The expression analysis of these miRNAs documented their roles in growth and development of Stevia. Furthermore, the detected miRNAs were found to target genes involved in plant growth, development, metabolism and signal transduction. This is the first study reporting these conserved miRNAs and their expression in Stevia.
authors.affiliation: Plant Metabolic Engineering Laboratory, Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Council of Scientific and Industrial Research, Palampur17606, India.
authors.forename: Praveen
authors.initials: P
authors.lastname: Guleria
authors.forename: Sudesh Kumar
authors.initials: SK
authors.lastname: Yadav
mesh_terms: D017124:Conserved Sequence / Q000235:genetics; D020224:Expressed Sequence Tags; D018506:Gene Expression Regulation, Plant; D035683:MicroRNAs / Q000737:chemistry / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D036625:Stevia / Q000235:genetics* / Q000378:metabolism
chemical_list: D035683:MicroRNAs