title: Sphingomyelin/phosphatidylcholine/cholesterol monolayers--analysis of the interactions in model membranes and Brewster Angle Microscopy experiments.
abstract: In this work the properties of two ternary sphingomyelin/phosphatidylcholine/cholesterol monolayers imitating erythrocyte membrane were studied at various content of sterol. Phosphatidylcholines chosen for experiments differ in the length of sn-1 saturated chain in the molecule (1-stearoyl-2-oleoyl-sn-glycero-3-phosphocholine-SOPC vs. 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine - POPC). Langmuir monolayer experiments combined with Brewster Angle Microscopy prove that for both investigated systems the most favorable effect of cholesterol appears at 30% of sterol in the film. However, the interactions between molecules at 50% of sterol are only slightly weaker as compared to those for 1:1:1 films. It was also found that only at higher sterol concentration appear differences in the ordering effect of cholesterol on the systems containing PC molecules of various length of sn-1 saturated chain. Although the differences in the properties of POPC versus SOPC-containing monolayers were found, similarities in the morphology of the respective systems and stoichiometry of thermodynamically the most favorable mixture allow one to conclude that both SM/POPC/Chol as well as SM/SOPC/Chol monolayer can be used to mimic raft systems.
authors.affiliation: Jagiellonian University, Faculty of Chemistry, Kraków, Poland. wydro@chemia.uj.edu.pl
authors.forename: Paweł
authors.initials: P
authors.lastname: Wydro
mesh_terms: D002784:Cholesterol / Q000737:chemistry*; D004910:Erythrocyte Membrane / Q000737:chemistry / Q000648:ultrastructure; D021962:Membrane Microdomains / Q000737:chemistry / Q000648:ultrastructure; D008567:Membranes, Artificial; D008853:Microscopy; D010713:Phosphatidylcholines / Q000737:chemistry*; D013109:Sphingomyelins / Q000737:chemistry*; D013499:Surface Properties; D013816:Thermodynamics
chemical_list: D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D013109:Sphingomyelins; C027891:1-stearoyl-2-oleoyl-sn-glycero-3-phosphocholine; D002784:Cholesterol; C028694:1-palmitoyl-2-oleoylphosphatidylcholine