title: Genetic  diversity of Metrodorea nigra (Rutaceae) from a small forest remnant in  Brazil assessed with microsatellite markers.
abstract: Metrodorea nigra (Rutaceae) is an endemic Brazilian tree of  great ecological importance, frequently found in the submontane  regions of ombrophilous dense and semideciduous  forests. This tree is useful for reforesting degraded areas and the wood can be  employed in construction. We developed 12 microsatellite markers from a genomic  library enriched for GA/CA repeats, for this species. Polymorphisms were  assessed in 40 trees of a highly fragmented population found in Cravinhos,  State of São Paulo, in southeastern Brazil. Among the 12 loci, 8 were  polymorphic and only one had fixed alleles in this population. The number of  alleles per locus and expected heterozygosity ranged from 2 to 11 and from  0.190 to 0.889, respectively. These results revealed moderate levels of  genetic variation in M. nigra population when compared to other tropical  species. Additionally, transferability of the 12 primers was tested in seven  other Brazilian Rutaceae tree species (endemics: M. stipularis, Galipea  jasminiflora, Esenbeckia leiocarpa and non-endemics: E. febrifuga, E. grandiflora, Balfourodendron riedelianum, Zanthoxylum  riedelianum). Transferability ranged among species, but at least 8 loci  (~67%) amplified in M. stipularis, demonstrating a high potential for  transferring microsatellite markers between species of the same genus in the  Rutaceae family.
authors.affiliation: Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil.
authors.forename: M C
authors.initials: MC
authors.lastname: Guidugli
authors.forename: R
authors.initials: R
authors.lastname: Ferreira-Ramos
authors.forename: A C B
authors.initials: AC
authors.lastname: de Sousa
authors.forename: F W
authors.initials: FW
authors.lastname: Cidade
authors.forename: T G
authors.initials: TG
authors.lastname: Marconi
authors.forename: M A
authors.initials: MA
authors.lastname: Mestriner
authors.forename: M
authors.initials: M
authors.lastname: Groppo
authors.forename: A L
authors.initials: AL
authors.lastname: Alzate-Marin
mesh_terms: D000483:Alleles; D001938:Brazil; D014644:Genetic Variation; D005828:Genetics, Population; D005838:Genotype; D018895:Microsatellite Repeats / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D027821:Rutaceae / Q000235:genetics*