title: Preparation of novel solid lipid microparticles loaded with gentamicin and its evaluation in vitro and in vivo.
abstract: OBJECTIVE
  To formulate and evaluate solid-reversed-micellar-solution (SRMS)-based solid lipid microparticles (SLMs) for intramuscular administration of gentamicin.
  
  
  METHODS
  SRMS formulated with Phospholipon® 90G and Softisan® 154 were used to prepare gentamicin-loaded SLMs. Characterizations based on size and morphology, stability and encapsulation efficiency (EE%) were carried out on the SLMs. In vitro release of gentamicin from the SLMs was performed in phosphate buffer while in vivo release studies were conducted in rats.
  
  
  RESULTS
  Maximum EE% of 90.0, 91.6 and 83.0% were obtained for SLMs formed with SRMS 1:1, 1:2 and 2:1, respectively. Stable, spherical and smooth SLMs of size range 9.80 ± 1.46 µm to 33.30 ± 6.42 µm were produced. The release of gentamicin in phosphate buffer varied widely with the lipid contents. Moreover, significant (p < 0.05) amount of gentamicin was released in vivo from the SLMs.
  
  
  CONCLUSIONS
  SRMS-based SLMs would likely offer a reliable means of delivering gentamicin intramuscularly.
authors.affiliation: School of Pharmacy, University of Nigeria, Nsukka 410001, Nigeria.
authors.forename: Emmanuel Chukwuebuka
authors.initials: EC
authors.lastname: Umeyor
authors.forename: Franklin Chimaobi
authors.initials: FC
authors.lastname: Kenechukwu
authors.forename: John Dike
authors.initials: JD
authors.lastname: Ogbonna
authors.forename: Salome Amarachi
authors.initials: SA
authors.lastname: Chime
authors.forename: Anthony
authors.initials: A
authors.lastname: Attama
mesh_terms: D000818:Animals; D002021:Buffers; D002152:Calorimetry, Differential Scanning / Q000379:methods; D002626:Chemistry, Pharmaceutical / Q000379:methods; D004337:Drug Carriers; D004339:Drug Compounding; D016503:Drug Delivery Systems; D005839:Gentamicins / Q000008:administration & dosage* / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007273:Injections, Intramuscular / Q000379:methods; D007700:Kinetics; D008055:Lipids / Q000737:chemistry*; D008823:Micelles; D008863:Microspheres; D010316:Particle Size; D010710:Phosphates / Q000737:chemistry; D051381:Rats; D013696:Temperature
chemical_list: D002021:Buffers; D004337:Drug Carriers; D005839:Gentamicins; D008055:Lipids; D008823:Micelles; D010710:Phosphates