title: Identification of endometriosis-related genes by representational difference analysis of cDNA.
abstract: BACKGROUND
  Accumulated evidence reveals that abnormally expressed genes in eutopic endometrium of endometriosis play a critical role in the pathogenesis of endometriosis.
  
  
  OBJECTIVE
  Identification of endometriosis-related genes for further revealing the pathogenesis of endometriosis and offering the basis for developing the molecular-targeted diagnosis and therapy of endometriosis.
  
  
  METHODS
  Forty women with endometriosis and forty control women without endometriosis during their secretory phase were selected for this study. cDNA representational difference analysis (cDNA-RDA) was performed to screen the up-regulated genes in eutopic endometrium samples of endometriosis (n = 10) compared with the controls (n = 10). To validate the results, MAT2A, the most abundantly expressed gene, was selected to detect mRNA and protein levels between eutopic endometrium of endometriosis (n = 40) and controls (n = 40) using immunohistochemistry, real-time fluorescent quantitative PCR and Western blotting.
  
  
  RESULTS
  Ten up-regulated genes were identified in eutopic endometria of endometriosis compared with controls. Among these genes, COX-2, BRAF, NRAS and CFL1 have already been reported to be associated with the endometriosis in previous studies. MAT2A, SEPT9, ATAD3A and CADM2 have been reported to be involved in other diseases but not in endometriosis. NAA15 and CCDC21 have not reported in any diseases. Further study showed that MAT2A protein was localised in both endometrial glandular and stromal cells. Compared with controls, the mRNA and protein levels of MAT2A were significantly higher in eutopic endometrium of endometriosis (P < 0.05).
  
  
  CONCLUSIONS
  cDNA-RDA can be used to effectively identify the endometriosis-related genes, which can provide novel experimental data and further understand the pathogenesis of endometriosis.
authors.affiliation: Department of Obstetrics & Gynecology, Shengjing Hospital Affiliated to China Medical University, Shenyang, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Chen
authors.forename: ChiYuan
authors.initials: C
authors.lastname: Zhang
authors.forename: YingHan
authors.initials: Y
authors.lastname: Chen
authors.forename: Jie
authors.initials: J
authors.lastname: Lou
authors.forename: DanBo
authors.initials: D
authors.lastname: Wang
mesh_terms: D000074183:ATPases Associated with Diverse Cellular Activities; D000123:Acetyltransferases / Q000096:biosynthesis / Q000235:genetics; D000251:Adenosine Triphosphatases / Q000096:biosynthesis / Q000235:genetics; D000328:Adult; D015815:Cell Adhesion Molecules / Q000096:biosynthesis / Q000235:genetics; D051338:Cofilin 1 / Q000096:biosynthesis / Q000235:genetics; D051546:Cyclooxygenase 2 / Q000096:biosynthesis / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics* / Q000378:metabolism; D004715:Endometriosis / Q000235:genetics* / Q000378:metabolism; D004717:Endometrium / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics; D008716:Methionine Adenosyltransferase / Q000096:biosynthesis / Q000235:genetics; D008875:Middle Aged; D024101:Mitochondrial Proteins / Q000096:biosynthesis / Q000235:genetics; D063209:N-Terminal Acetyltransferase A; D063213:N-Terminal Acetyltransferase E; D048493:Proto-Oncogene Proteins B-raf / Q000096:biosynthesis / Q000235:genetics; D058112:Septins / Q000096:biosynthesis / Q000235:genetics; D017154:Stromal Cells / Q000378:metabolism; D015854:Up-Regulation
chemical_list: C549686:ATAD3A protein, human; C556123:CADM2 protein, human; C495810:CFL1 protein, human; D015815:Cell Adhesion Molecules; D051338:Cofilin 1; D018076:DNA, Complementary; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; C463022:NAA15 protein, human; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D000123:Acetyltransferases; D063209:N-Terminal Acetyltransferase A; D063213:N-Terminal Acetyltransferase E; C527161:MAT2A protein, human; D008716:Methionine Adenosyltransferase; C482119:BRAF protein, human; D048493:Proto-Oncogene Proteins B-raf; D000251:Adenosine Triphosphatases; C474315:SEPTIN9 protein, human; D058112:Septins; D000074183:ATPases Associated with Diverse Cellular Activities