title: Isolation of siRNA target by biotinylated siRNA reveals that human CCDC12 promotes early erythroid differentiation.
abstract: Erythroid differentiation is a tightly regulated multi-step process that has not been fully elucidated. We previously reported that a siRNA screened from random siRNA library, siRNA clone-67, induced erythroid differentiation in human erythroleukemia K-562cell line. Here we identified that human CCDC12 (coiled-coil domain containing 12) is a target of siRNA clone-67, by target capture with biotinylated siRNA. Over-expression of CCDC12 in K-562cell up-regulated the expression of CD235, ε-globin and γ-globin, accelerated cell growth, and slightly down-regulated the expression of GATA-2. Knockdown of CCDC12 slowed down the cell growth. These data indicate that CCDC12 is a new participant that promotes early erythroid differentiation.
authors.affiliation: Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (CAMS), School of Basic Medicine, Peking Union Medical College (PUMC), National Laboratory of Medical Molecular Biology, Beijing, China.
authors.forename: Cuiqing
authors.initials: C
authors.lastname: Fan
authors.forename: Lin
authors.initials: L
authors.lastname: Dong
authors.forename: Ning
authors.initials: N
authors.lastname: Zhu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Xiong
authors.forename: Jiewen
authors.initials: J
authors.lastname: Zhang
authors.forename: Le
authors.initials: L
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Shen
authors.forename: Xiaoyin
authors.initials: X
authors.lastname: Zhang
authors.forename: Meihong
authors.initials: M
authors.lastname: Chen
mesh_terms: D019921:Biotinylation; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics*; D049109:Cell Proliferation; D041905:Erythroid Cells / Q000187:drug effects / Q000378:metabolism; D004920:Erythropoiesis / Q000187:drug effects / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000235:genetics; D055785:Gene Knockdown Techniques / Q000379:methods; D050437:Genes, Developmental / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D006801:Humans; D020014:K562 Cells; D011506:Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism / Q000502:physiology; D034741:RNA, Small Interfering / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D013997:Time Factors; D014162:Transfection
chemical_list: C572768:CCDC12 protein, human; D011506:Proteins; D034741:RNA, Small Interfering