title: Static biofilm cultures of Gram-positive pathogens grown in a microtiter format used for anti-biofilm drug discovery.
abstract: An in vitro assay is presented for culturing staphylococcal biofilms and biofilms of nonmotile Gram-positive bacteria under static conditions in microtiter assay plates, and for the quantification of biofilm growth, using a simple staining procedure that measures amounts of bacterial cells and extracellular matrix. This basic assay can be adapted readily to study several aspects of biofilm formation, for high-throughput screening to identify small molecule inhibitors of biofilm formation or biofilm-defective mutants, and for quantifying the anti-biofilm activity of biofilm inhibitors.
authors.affiliation: Microbiotix, Anti-Infectives R&D, Worcester, Massachusetts, USA.
authors.forename: Steven M
authors.initials: SM
authors.lastname: Kwasny
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Opperman
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000502:physiology; D001431:Bacteriological Techniques / Q000379:methods*; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development*; D001681:Biological Assay / Q000379:methods*; D003470:Culture Media; D055808:Drug Discovery / Q000379:methods*; D006094:Gram-Positive Bacteria / Q000254:growth & development*; D013048:Specimen Handling / Q000379:methods; D013210:Staphylococcus / Q000254:growth & development
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media