title: Translational control of TWIST1 expression in MCF-10A cell lines recapitulating breast cancer progression.
abstract: TWIST1 is a highly conserved basic helix-loop-helix transcription factor that promotes epithelial-mesenchymal transition (EMT). Its misregulation has been observed in various types of tumors. Using the MCF-10A-series of cell lines that recapitulate the early stages of breast cancer formation and EMT, we found TWIST1 to be upregulated during EMT and downregulated early in carcinogenesis. The TWIST1 3'UTR contains putative regulatory elements, including miRNA target sites and two cytoplasmic polyadenylation elements (CPE). We found that miR-580, CPEB1, and CPEB2 act as negative regulators of TWIST1 expression in a sequence-specific and additive/cooperative manner.
authors.affiliation: Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark.
authors.forename: M-L
authors.initials: ML
authors.lastname: Nairismägi
authors.forename: A
authors.initials: A
authors.lastname: Vislovukh
authors.forename: Q
authors.initials: Q
authors.lastname: Meng
authors.forename: G
authors.initials: G
authors.lastname: Kratassiouk
authors.forename: C
authors.initials: C
authors.lastname: Beldiman
authors.forename: M
authors.initials: M
authors.lastname: Petretich
authors.forename: R
authors.initials: R
authors.lastname: Groisman
authors.forename: E-M
authors.initials: EM
authors.lastname: Füchtbauer
authors.forename: A
authors.initials: A
authors.lastname: Harel-Bellan
authors.forename: I
authors.initials: I
authors.lastname: Groisman
mesh_terms: D020413:3' Untranslated Regions; D001665:Binding Sites; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D002460:Cell Line; D002465:Cell Movement; D018450:Disease Progression; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D006801:Humans; D049410:Luciferases, Renilla / Q000096:biosynthesis / Q000235:genetics; D035683:MicroRNAs / Q000502:physiology*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D014176:Protein Biosynthesis; D034622:RNA Interference; D016601:RNA-Binding Proteins / Q000378:metabolism / Q000502:physiology; D014157:Transcription Factors / Q000378:metabolism / Q000502:physiology; D051879:Twist-Related Protein 1 / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation; D039221:mRNA Cleavage and Polyadenylation Factors / Q000378:metabolism / Q000502:physiology
chemical_list: D020413:3' Untranslated Regions; C490386:CPEB1 protein, human; C554938:CPEB2 protein, human; C577667:MIRN580 microRNA, human; D035683:MicroRNAs; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins; C103173:TWIST1 protein, human; D014157:Transcription Factors; D051879:Twist-Related Protein 1; D039221:mRNA Cleavage and Polyadenylation Factors; D049410:Luciferases, Renilla