title: [Viral etiology of 1165 hospitalized children with acute lower respiratory tract infection].
abstract: OBJECTIVE
  To explore the viral etiology of acute low respiratory tract infection (ALRTI) among hospitalized children in Changsha of Hunan Province of China.
  
  
  METHODS
  Nasopharyngeal aspirates were collected from 1165 hospitalized children with ALRTI in Changsha from September 2007 to August 2008. Respiratory syncytin virus (RSV), human rhinovirus (HRV), influenza virus A (IFVA), influenza virus B (IFVB), parainfluenza 1-3 (PIV 1-3), human metapneumovirus (hMPV), human coronaviruses NL63 (HCoV-NL63), and human coronaviruses HKU1 (HCoV-HKU1) were detected by reverse transcription polymerase chain reaction (RT-PCR). Adenovirus (ADV) and human bocavirus (HBoV) were detected by standard polymerase chain reaction (PCR). WU polyomaviruses (WUPyV) and KI polyomaviruses(KIPyV) were detected by nested PCR. The positive samples further underwent genetic sequencing.
  
  
  RESULTS
  Among the 1165 nasopharyngeal aspirates, viruses were detected in 871 samples (74.76%), among which RSV (27.03%) was the most common virus, followed by HRV (17.33%), PIV3 (13.73%), HBoV (8.67%) and hMPV (6.52%). The overall positive rate of viral detection showed no significant differences between males and females (X2=2.241, P=0.134), whereas the positive rates of PIV3, hMPV, and HBoV in males were higher than in females. The positive rate of viral detection showed significant differences among different age groups (X2=10.934, P=0.027), and the highest positive rate was noted in the age group of 6 months to 1 year. Furthermore, the overall positive rate of viral detection showed a significant difference in term of seasonal distribution, with a peak prevalence in winter.
  
  
  CONCLUSIONS
  Virues predominate in the etiology of pediatric ALRTI in Changsha, and RSV, HRV and PIV3 are the main viruses for ALRTI. HBoV and hMPV have become increasingly important. Viral infection-associated ALRTI shows a prevail in the age group of 6 months to 1 year as well as in winter.
authors.affiliation: Department of Pediatrics, People's Hospital of Hunan Province, Changsha 410005, China.
authors.forename: Ni-Guang
authors.initials: NG
authors.lastname: Xiao
authors.forename: Bing
authors.initials: B
authors.lastname: Zhang
authors.forename: Zhao-Jun
authors.initials: ZJ
authors.lastname: Duan
authors.forename: Zhi-Ping
authors.initials: ZP
authors.lastname: Xie
authors.forename: Qiong-Hua
authors.initials: QH
authors.lastname: Zhou
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Zhong
authors.forename: Han-Chun
authors.initials: HC
authors.lastname: Gao
authors.forename: Xiao-Fang
authors.initials: XF
authors.lastname: Ding
authors.forename: Sai-Zhen
authors.initials: SZ
authors.lastname: Zeng
authors.forename: Han
authors.initials: H
authors.lastname: Huang
authors.forename: Yun-De
authors.initials: YD
authors.lastname: Hou
mesh_terms: D000293:Adolescent; D017677:Age Distribution; D002648:Child; D002673:Child, Hospitalized; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D009305:Nasopharynx / Q000821:virology; D012141:Respiratory Tract Infections / Q000209:etiology / Q000821:virology*; D012621:Seasons; D017678:Sex Distribution; D014780:Viruses / Q000302:isolation & purification*