title: Identification of a novel HIV type 1 circulating recombinant form (CRF52_01B) in Southeast Asia.
abstract: Thirty HIV-1 URF_01AE/ B' complete or nearly full-length genome sequences sampled within Southeast Asia were obtained from the Los Alamos HIV Sequence Database. Phylogenetic and recombinant analyses revealed that three sequences indeed displayed the identical recombinant structure. Of note, the three subjects, harboring novel CRF01_AE/B recombinants, did not have apparent epidemiological linkage. They fulfilled the criteria for the designation of a new circulating recombinant form (CRF) and constituted the 52nd CRF identified in the worldwide HIV-1 pandemic. In this chimera, two short subtype B segments were inserted into a backbone of CRF_01AE. The breakpoints corresponded to HXB2 nucleotide positions 2930, 3251, 8521, and 9004 approximately. This CRF is the first one identified by neatening and analyzing the sequences already presented in the Los Alamos HIV Sequence Database. This indicates that we should pay attention not only to explicit subtype sequences but also to those classified as a unique recombinant form (URF) so far.
authors.affiliation: Department of AIDS Research, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, China.
authors.forename: Yongjian
authors.initials: Y
authors.lastname: Liu
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.forename: Zuoyi
authors.initials: Z
authors.lastname: Bao
authors.forename: Hanping
authors.initials: H
authors.lastname: Li
authors.forename: Daomin
authors.initials: D
authors.lastname: Zhuang
authors.forename: Siyang
authors.initials: S
authors.lastname: Liu
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Wang
authors.forename: Tianyi
authors.initials: T
authors.lastname: Li
authors.forename: Lei
authors.initials: L
authors.lastname: Jia
authors.forename: Shaomin
authors.initials: S
authors.lastname: Yang
authors.forename: Jingyun
authors.initials: J
authors.lastname: Li
mesh_terms: D000328:Adult; D001210:Asia, Southeastern / Q000453:epidemiology; D001483:Base Sequence; D005260:Female; D005838:Genotype; D006679:HIV Seropositivity / Q000453:epidemiology / Q000635:transmission / Q000821:virology*; D015497:HIV-1 / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D011995:Recombination, Genetic / Q000235:genetics*