title: Chronic rhinosinusitis with nasal polyps: a proteomic analysis.
abstract: OBJECTIVE
  Chronic rhinosinusitis with nasal polyps (CRSwNP) is a severe subtype of chronic rhinosinusitis that can affect patients despite medical and surgical interventions. The purpose of this study was to utilize the techniques of proteomics to investigate differences in protein abundance within the sinonasal mucosa of patients with CRSwNP compared to healthy controls.
  
  
  METHODS
  In a case-control study at a tertiary-care academic medical center, sinonasal mucosa was harvested from 3 patients with CRSwNP and 3 control patients undergoing transsphenoidal excision of pituitary tumors. Two-dimensional gel electrophoresis was used to identify proteins with elevated or reduced abundance in CRSwNP patients compared to controls. The proteins showing the greatest abundance differences were characterized by mass spectrometry.
  
  
  RESULTS
  More than 300 differentially abundant proteins (p < or = 0.05) were identified. Many of these protein species were involved in the host inflammatory response. Proteins up-regulated in CRSwNP patients included eosinophil lysophospholipase by a ratio (R) of 18.13, RHO-GDP dissociation inhibitor 2 (R = 2.80), and apolipoprotein A-1 (R = 1.73). Down-regulated proteins in CRSwNP patients included catalase (R = -5.87), annexin A1 (R = -6.27), and keratin II-8 (R = -6.73). A detailed analysis of additional protein species is outlined.
  
  
  CONCLUSIONS
  The proteomic approach allows detection of significant differences in protein abundance in CRSwNP and provides unique insight into the pathophysiology of this common disease.
authors.affiliation: Department of Surgery, Division of Otolaryngology University of Wisconsin Hospital and Clinics, Madison, Wisconsin 53792, USA.
authors.forename: David C
authors.initials: DC
authors.lastname: Upton
authors.forename: Nathan V
authors.initials: NV
authors.lastname: Welham
authors.forename: John S
authors.initials: JS
authors.lastname: Kuo
authors.forename: Jeffery W
authors.initials: JW
authors.lastname: Walker
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Pasic
mesh_terms: D000328:Adult; D017305:Annexin A1; D016632:Apolipoprotein A-I; D016022:Case-Control Studies; D002908:Chronic Disease; D003598:Cytoskeletal Proteins / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D009298:Nasal Polyps / Q000150:complications*; D040901:Proteomics; D012220:Rhinitis / Q000150:complications* / Q000503:physiopathology*; D012852:Sinusitis / Q000150:complications* / Q000503:physiopathology*; D014057:Tomography, X-Ray Computed
chemical_list: D017305:Annexin A1; D016632:Apolipoprotein A-I; D003598:Cytoskeletal Proteins