title: Direct effects of conjugated linoleic acid isomers on P815 mast cells in vitro.
abstract: Conjugated linoleic acid (CLA) is a dietary fatty acid which causes extensive remodeling and mast cell recruitment in the mouse mammary gland. Two CLA isomers, 9,11- and 10,12-CLA, have differing effects in vivo, with only 10,12-CLA increasing mast cell number. The purpose of this project is to test the hypothesis that CLA acts directly on the mast cell. The P815 mastocytoma cell line was assayed for the effects of CLA on mast cell number, proliferation, apoptosis, and differentiation. Both CLA isomers decreased viable mast cell number, with no effect on membrane integrity, or cell cycle distribution. 10,12-CLA induced an increase in apoptosis, assessed by Annexin-FITC binding. Both isomers increased mast cell granularity, and secretion of MMP-9. The complex effects of CLA isomers on mast cells in the mammary gland are distinct from direct effects on mast cells in vitro, and may require interactions between multiple cell types present in vivo.
authors.affiliation: Department of Biotechnical and Clinical Laboratory Sciences, School of Medicine and Biomedical Sciences, State University of New York at Buffalo, Buffalo, NY 14214, USA. pmwelch@buffalo.edu
authors.forename: Siddharth
authors.initials: S
authors.lastname: Krishnan
authors.forename: Joshua
authors.initials: J
authors.lastname: Russell
authors.forename: MaryLou
authors.initials: M
authors.lastname: Bodziak
authors.forename: Stephen
authors.initials: S
authors.lastname: Koury
authors.forename: Patricia
authors.initials: P
authors.lastname: Masso-Welch
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003594:Cytoplasmic Granules / Q000187:drug effects; D044243:Linoleic Acids, Conjugated / Q000494:pharmacology*; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism*; D051379:Mice
chemical_list: D044243:Linoleic Acids, Conjugated