title: [Modulation of activated oncogene c-kit expression with RNA-interference].
abstract: Hyperexpression of oncogene c-kit is found in 80% patients with acute myeloid leukemia (AML). The transgenic model cell line expressing the oncogene c-kit was obtained by transduction with recombinant retrovirus. We have designed small interfering RNAs (siRNA) efficiently suppressing the expression of activated oncogene c-kit. Further small hairpin RNAs (shRNA) targeting c-kit mRNA were designed and expressed in lentiviral vectors. We report a stable reduction in c-kit expression following the introduction of shRNAs into model cells as well as Kasumi-1 cells from the patient with AML.
authors.forename: P V
authors.initials: PV
authors.lastname: Spirin
authors.forename: N A
authors.initials: NA
authors.lastname: Nikitenko
authors.forename: T D
authors.initials: TD
authors.lastname: Lebedev
authors.forename: P M
authors.initials: PM
authors.lastname: Rubtsov
authors.forename: C
authors.initials: C
authors.lastname: Stocking
authors.forename: V S
authors.initials: VS
authors.lastname: Prasolov
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D015973:Gene Expression Regulation, Leukemic; D020868:Gene Silencing; D005822:Genetic Vectors; D057809:HEK293 Cells; D006801:Humans; D016086:Lentivirus; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D051379:Mice; D019009:Proto-Oncogene Proteins c-kit / Q000037:antagonists & inhibitors* / Q000235:genetics; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics*; D015533:Transcriptional Activation
chemical_list: D012333:RNA, Messenger; D034741:RNA, Small Interfering; D019009:Proto-Oncogene Proteins c-kit