title: Methanoculleus spp. as a biomarker of methanogenic activity in swine manure storage tanks.
abstract: Greenhouse gas emissions represent a major problem associated with manure management in the livestock industry. A prerequisite to mitigate methane emissions occurring during manure storage is a clearer understanding of how the microbial consortia involved in methanogenesis function. Here, we have examined manure stored in outdoor tanks from two different farms, at different locations and depths. Physico-chemical and microbiological characterization of these samples indicated differences between each tank, as well as differences within each tank dependent on the depth of sampling. The dynamics of both the bacterial and archaeal communities within these samples were monitored over a 150-day period of anaerobic incubation to identify and track emerging microorganisms, which may be temporally important in the methanogenesis process. Analyses based on DNA fingerprinting of microbial communities identified trends common among all samples as well as trends specific to certain samples. All archaeal communities became enriched with Methanoculleus spp. over time, indicating that the hydrogenotrophic pathway of methanogenesis predominated. Although the emerging species differed in samples obtained from shallow depths compared to deep samples, the temporal enrichment of Methanoculleus suggests that this genus may represent a relevant indicator of methanogenic activity in swine manure storage tanks.
authors.affiliation: Dairy and Swine Research and Development Centre, Agriculture and Agri-Food Canada, Sherbrooke, QC, Canada.
authors.forename: Maialen
authors.initials: M
authors.lastname: Barret
authors.forename: Nathalie
authors.initials: N
authors.lastname: Gagnon
authors.forename: Bruno
authors.initials: B
authors.lastname: Morissette
authors.forename: Edward
authors.initials: E
authors.lastname: Topp
authors.forename: Martin
authors.initials: M
authors.lastname: Kalmokoff
authors.forename: Stephen P J
authors.initials: SP
authors.lastname: Brooks
authors.forename: Fernando
authors.initials: F
authors.lastname: Matias
authors.forename: Daniel I
authors.initials: DI
authors.lastname: Massé
authors.forename: Lucie
authors.initials: L
authors.lastname: Masse
authors.forename: Guylaine
authors.initials: G
authors.lastname: Talbot
mesh_terms: D000693:Anaerobiosis; D000818:Animals; D001483:Base Sequence; D001673:Biodegradation, Environmental; D016172:DNA Fingerprinting; D008372:Manure / Q000382:microbiology*; D008697:Methane / Q000378:metabolism*; D019638:Methanomicrobiaceae / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D008969:Molecular Sequence Data; D013552:Swine
chemical_list: D008372:Manure; D008697:Methane