title: In vivo antioxidative property, antimicrobial and wound healing activity of flower extracts of Pyrostegia venusta (Ker Gawl) Miers.
abstract: BACKGROUND
  Pyrostegia venusta (Ker Gawl) Miers. (Bignoniaceae), has been traditionally used as a remedy for treating white patches and infections on the skin (leukoderma, vitiligo).
  
  
  OBJECTIVE
  To investigate wound healing and antimicrobial activity of flower extract of Pyrostegia venusta, including in vivo antioxidant activity.
  
  
  METHODS
  Methanolic extracts of Pyrostegia venusta flowers were studied for wound healing efficiency along with its effect on pro-inflammatory and anti-inflammatory cytokines was assessed using excision and incision model of wound repair in Wistar rats. Healing was assessed by the rate of wound contraction, tensile strength, breaking strength, hydroxyproline and hexosamine content. Antimicrobial activity of the flower extract against twelve microorganisms was also assessed. In vivo antioxidant activity was performed to understand the mechanism of wound healing potency.
  
  
  RESULTS
  The results indicated that Pyrostegia venusta extract has potent wound healing capacity as evident from the wound contraction and increased tensile strength. Hydroxyproline and hexosamine expression were also correlative with the healing pattern observed. Pyrostegia venusta extract exhibited moderate antimicrobial activity against the organisms: Bacillus subtilis, Staphylococcus epidermidis, Staphylococcus pyogenes, Staphylococcus aureus, Escherichia coli, Micrococcus luteus, Enterobacter aerogenes, Salmonella typhi, Pseudomonas aeruginosa, Candida albicans, Aspergillus niger and Candida tropicana. During early wound healing phase TNF-α and IL-6 level were found to be up regulated by Pyrostegia venusta treatment.
  
  
  CONCLUSIONS
  Increased wound contraction and tensile strength, augmented hydroxyproline and hexosamine content along with antioxidative activity and moderate antimicrobial activity support the early wound healing exhibited by Pyrostegia venusta flower extract. Induction in cytokine production may be one of the mechanisms involved in accelerating the wound healing by Pyrostegia venusta extract. Results suggest that Pyrostegia venusta may be useful in the tropical management of wound healing.
authors.affiliation: Department of Microbiology, Barkatullah University, Hoshangabad Road, Habibganj, Bhopal 462024, Madhya Pradesh, India. purviroy@gmail.com
authors.forename: Purabi
authors.initials: P
authors.lastname: Roy
authors.forename: Sarika
authors.initials: S
authors.lastname: Amdekar
authors.forename: Avnish
authors.initials: A
authors.lastname: Kumar
authors.forename: Rambir
authors.initials: R
authors.lastname: Singh
authors.forename: Poonam
authors.initials: P
authors.lastname: Sharma
authors.forename: Vinod
authors.initials: V
authors.lastname: Singh
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D001419:Bacteria / Q000187:drug effects; D029402:Bignoniaceae; D035264:Flowers; D005658:Fungi / Q000187:drug effects; D006595:Hexosamines / Q000378:metabolism; D006909:Hydroxyproline / Q000378:metabolism; D015848:Interleukin-5 / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000187:drug effects / Q000293:injuries / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects*; D014947:Wounds and Injuries / Q000188:drug therapy* / Q000378:metabolism
chemical_list: D000890:Anti-Infective Agents; D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D006595:Hexosamines; D015848:Interleukin-5; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D006909:Hydroxyproline