title: Lack of association of conjunctival MALT lymphoma with Chlamydiae or Helicobacter pylori in a cohort of Chinese patients.
abstract: BACKGROUND
  This study was conducted to detect microbial pathogens in conjunctival mucosa-associated lymphoid tissue (MALT) lymphoma specimens in an attempt to determine possible associations between conjunctival MALT lymphoma and microbial infections.
  
  
  METHODS
  Using PCR technique, freshly obtained tumor specimens from 16 cases of conjunctival MALT lymphoma, as confirmed by postoperative pathology, were analyzed for DNA of Chlamydia psittaci (C. psittaci), Chlamydia trachomatis (C. trachomatis), Chlamydia pneumoniae (C. pneumoniae) and Helicobacter pylori (H. pylori). Synthetic C. psittaci, C. trachomatis, C. pneumoniae and H. pylori DNA were used as positive control, and blank plasmid DNA as negative control.
  
  
  RESULTS
  Electrophoresis showed that no bands corresponding to the positive control were observed in the specimens, indicating that no DNA of the 4 microorganisms was detected in the specimens of the 16 cases of conjunctival MALT lymphoma.
  
  
  CONCLUSIONS
  The PCR technique was able to detect the positive control quickly and accurately, but the results of PCR in analyzing the 16 specimens were negative, indicating that there is no association between conjunctival MALT lymphoma and the 4 microorganisms in Chinese patients.
authors.affiliation: Department of Ophthalmology, Shanghai Changzheng Hospital, 2nd Military Medical University, Shanghai, China.
authors.forename: Ji-Ping
authors.initials: JP
authors.lastname: Cai
authors.forename: Jin-Wei
authors.initials: JW
authors.lastname: Cheng
authors.forename: Xiao-Ye
authors.initials: XY
authors.lastname: Ma
authors.forename: Yu-Zhen
authors.initials: YZ
authors.lastname: Li
authors.forename: You
authors.initials: Y
authors.lastname: Li
authors.forename: Xiao
authors.initials: X
authors.lastname: Huang
authors.forename: Rui-Li
authors.initials: RL
authors.lastname: Wei
mesh_terms: D001483:Base Sequence; D002681:China; D002689:Chlamydia / Q000235:genetics / Q000302:isolation & purification*; D015331:Cohort Studies; D003230:Conjunctival Neoplasms / Q000382:microbiology*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis; D016480:Helicobacter pylori / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D018442:Lymphoma, B-Cell, Marginal Zone / Q000382:microbiology*; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial