title: Development and characterization of novel cell lines from Etroplus suratensis and their applications in virology, toxicology and gene expression.
abstract: Four novel cell lines from tissues of eye, gill, kidney and brain of Etroplus suratensis were developed and characterized. The cell lines of eye, gill, kidney and brain were sub-cultured for 245, 185, 170 and 90 passages, respectively, since 2008. These cell lines showed predominantly epithelial-like cells. Effects of temperature and foetal bovine serum concentration on the growth of these cell lines were examined and optimum growth was found at the temperature of 28° C with 20% foetal bovine serum. All the four cell lines were successfully cryopreserved and revived at different passage levels. Cell-cycle analysis of these cell lines was carried out by fluorescence-activated cell sorting. Polymerase chain reaction (PCR) products obtained from the cells and tissues of E. suratensis with primers specific to the conserved region of 16S ribosomal RNA and cytochrome oxidase I genes of E. suratensis revealed the origin of cell lines from E. suratensis. Antibodies raised against the tissues and cells of eye, kidney and gill were highly cross reacted to their specific tissue and cells of E. suratensis. Chromosomal analysis revealed that E. suratensis cells have a normal diploid karyotype with 2n = 48. The cells of these cell lines were successfully transfected with pEGFP vector DNA. The eye (IEE), gill (IEG) and kidney (IEK) cell lines were found to be susceptible to nodavirus but resistant to infectious pancreatic necrosis virus (IPNV). The cells of gill, kidney and eye were applied to test the cytotoxicity of tannery effluents.
authors.affiliation: Aquaculture Biotechnology Division, C. Abdul Hakeem College, Melvisharam 632 509, Tamilnadu, India.
authors.forename: V
authors.initials: V
authors.lastname: Sarath Babu
authors.forename: V
authors.initials: V
authors.lastname: Chandra
authors.forename: K S N
authors.initials: KS
authors.lastname: Nambi
authors.forename: S A
authors.initials: SA
authors.lastname: Majeed
authors.forename: G
authors.initials: G
authors.lastname: Taju
authors.forename: M S
authors.initials: MS
authors.lastname: Patole
authors.forename: A S S
authors.initials: AS
authors.lastname: Hameed
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology; D002453:Cell Cycle; D002460:Cell Line / Q000166:cytology / Q000187:drug effects / Q000821:virology; D023681:Cichlids; D015925:Cryopreservation; D003470:Culture Media / Q000737:chemistry; D005123:Eye / Q000166:cytology; D005434:Flow Cytometry; D005880:Gills / Q000166:cytology; D018061:Infectious pancreatic necrosis virus; D059785:Karyotype; D007668:Kidney / Q000166:cytology; D016133:Polymerase Chain Reaction; D061251:Primary Cell Culture; D013696:Temperature; D018675:Toxicity Tests; D014162:Transfection
chemical_list: D003470:Culture Media