title: [Activation of the phosphatidylinositol-3'-kinase pathway with lectin-induced signal through sialo-containing glycoproteins of leukocyte membranes under type 1 diabetes mellitus].
abstract: The influence of wortmannin and sialospecific lectins on the translocation of p85alpha regulatory subunit of phosphatidylinositol-3'-kinase (PI-3'-kinase) between membrane and cytosolic fractions of the mononuclear and polymorphonuclear leukocytes in healthy donors and patients with type 1 diabetes mellitus (DM) was investigated. It was found out that under type 1 DM PI-3'-kinase takes active part in the transduction of lectin-induced signal through membrane glycoprotein receptors that contain terminal sialic acids linked to subterminal carbohydrate residues with (alpha2-->6) glycosidic bond.
authors.forename: N O
authors.initials: NO
authors.lastname: Sybirna
authors.forename: M I
authors.initials: MI
authors.lastname: Zdioruk
authors.forename: I V
authors.initials: IV
authors.lastname: Brodiak
authors.forename: M L
authors.initials: ML
authors.lastname: Bars'ka
authors.forename: O I
authors.initials: OI
authors.lastname: Vovk
mesh_terms: D000328:Adult; D000730:Androstadienes / Q000494:pharmacology; D015153:Blotting, Western; D002458:Cell Fractionation; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D003922:Diabetes Mellitus, Type 1 / Q000201:enzymology* / Q000473:pathology; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation / Q000187:drug effects; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000378:metabolism; D037102:Lectins / Q000494:pharmacology; D007962:Leukocytes / Q000187:drug effects / Q000201:enzymology* / Q000473:pathology; D058539:Phosphatidylinositol 3-Kinase / Q000378:metabolism*; D000081082:Phosphoinositide-3 Kinase Inhibitors; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D021122:Protein Subunits / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D012794:Sialic Acids / Q000737:chemistry / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D000077191:Wortmannin
chemical_list: D000730:Androstadienes; D006023:Glycoproteins; D007328:Insulin; D037102:Lectins; D000081082:Phosphoinositide-3 Kinase Inhibitors; D047428:Protein Kinase Inhibitors; D021122:Protein Subunits; D011956:Receptors, Cell Surface; D012794:Sialic Acids; D058539:Phosphatidylinositol 3-Kinase; D000077191:Wortmannin