title: Conjugation of cell-penetrating peptides leads to identification of anti-HIV peptides from matrix proteins.
abstract: Compounds which inhibit the HIV-1 replication cycle have been found amongst fragment peptides derived from an HIV-1 matrix (MA) protein. Overlapping peptide libraries covering the whole sequence of MA were designed and constructed with the addition of an octa-arginyl group to increase their cell membrane permeability. Imaging experiments with fluorescent-labeled peptides demonstrated these peptides with an octa-arginyl group can penetrate cell membranes. The fusion of an octa-arginyl group was proven to be an efficient way to find active peptides in cells such as HIV-inhibitory peptides.
authors.affiliation: Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo 101-0062, Japan.
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Narumi
authors.forename: Mao
authors.initials: M
authors.lastname: Komoriya
authors.forename: Chie
authors.initials: C
authors.lastname: Hashimoto
authors.forename: Honggui
authors.initials: H
authors.lastname: Wu
authors.forename: Wataru
authors.initials: W
authors.lastname: Nomura
authors.forename: Shintaro
authors.initials: S
authors.lastname: Suzuki
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Tanaka
authors.forename: Joe
authors.initials: J
authors.lastname: Chiba
authors.forename: Naoki
authors.initials: N
authors.lastname: Yamamoto
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Murakami
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Tamamura
mesh_terms: D000595:Amino Acid Sequence; D019380:Anti-HIV Agents / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology*; D002463:Cell Membrane Permeability; D057846:Cell-Penetrating Peptides / Q000737:chemistry* / Q000235:genetics; D002942:Circular Dichroism; D006678:HIV / Q000187:drug effects*; D006367:HeLa Cells; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019151:Peptide Library
chemical_list: D019380:Anti-HIV Agents; D057846:Cell-Penetrating Peptides; D019151:Peptide Library