title: Safety, immunogenicity, and efficacy of an alphavirus replicon-based swine influenza virus hemagglutinin vaccine.
abstract: A single-cycle, propagation-defective replicon particle (RP) vaccine expressing a swine influenza virus hemagglutinin (HA) gene was constructed and evaluated in several different animal studies. Studies done in both the intended host (pigs) and non-host (mice) species demonstrated that the RP vaccine is not shed or spread by vaccinated animals to comingled cohorts, nor does it revert to virulence following vaccination. In addition, vaccinated pigs develop both specific humoral and IFN-γ immune responses, and young pigs are protected against homologous influenza virus challenge.
authors.affiliation: Harrisvaccines, Inc., Ames, IA 50010, USA. rvanderveen@harrisvaccines.com
authors.forename: Ryan L
authors.initials: RL
authors.lastname: Vander Veen
authors.forename: Alan T
authors.initials: AT
authors.lastname: Loynachan
authors.forename: Mark A
authors.initials: MA
authors.lastname: Mogler
authors.forename: Brandon J
authors.initials: BJ
authors.lastname: Russell
authors.forename: D L Hank
authors.initials: DL
authors.lastname: Harris
authors.forename: Kurt I
authors.initials: KI
authors.lastname: Kamrud
mesh_terms: D000524:Alphavirus / Q000235:genetics / Q000276:immunology*; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000917:Antibody Formation; D003588:Cytopathogenic Effect, Viral; D005260:Female; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000276:immunology*; D007252:Influenza Vaccines / Q000235:genetics / Q000276:immunology*; D007371:Interferon-gamma / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009975:Orthomyxoviridae / Q000276:immunology / Q000472:pathogenicity; D009976:Orthomyxoviridae Infections / Q000276:immunology / Q000517:prevention & control*; D012093:Replicon; D013552:Swine / Q000276:immunology; D014774:Virulence; D017201:Virus Shedding
chemical_list: D000914:Antibodies, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines; D007371:Interferon-gamma