title: [A method of acutely isolating rat dorsal root ganglion neurons for patch-clamp study of single-channel].
abstract: OBJECTIVE
  To establish a method of acutely isolating dorsal root ganglion (DRG) neurons for patch clamp study of single-channel.
  
  
  METHODS
  DRG neurons of rats were acutely isolated by enzymatic digestion and mechanical blowing.
  
  
  RESULTS
  The acutely isolated DRG cells were easy to form the higher sealing resistance (> 5G Omega), which lowered noise level, so that pA-level single channel currents could be recorded.
  
  
  CONCLUSIONS
  The acutely isolated DRG neurons in this study are an ideal for patch-clamp study of single-channel.
authors.affiliation: Jiangsu Provincial Key Laboratory of Anesthesiology, Xuzhou Medical College, Xuzhou 221002, China.
authors.forename: Cheng-Biao
authors.initials: CB
authors.lastname: Zhang
authors.forename: Xiao-Lu
authors.initials: XL
authors.lastname: Yu
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Pan
authors.forename: Cui-Jie
authors.initials: CJ
authors.lastname: Shao
authors.forename: Jun-Li
authors.initials: JL
authors.lastname: Cao
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000379:methods*; D005260:Female; D005727:Ganglia, Spinal / Q000166:cytology*; D015640:Ion Channel Gating / Q000502:physiology*; D007473:Ion Channels; D008297:Male; D009474:Neurons / Q000166:cytology* / Q000502:physiology; D018408:Patch-Clamp Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D007473:Ion Channels