title: Analysis of cell dispersion and migration by video-microscopy.
abstract: In different physiopathological situations such as embryogenesis, wound repair and tumor invasion, isolated cells, or cell populations exhibit changes to their normal behavior and may acquire different migratory phenotypes. Live-cell imaging associated with the use of appropriate in vitro models in culture has become a powerful analytical tool for studying factors involved in cell migration and in cell-to-cell interactions. The scope of this chapter is to give an overview of in vitro models of cell migration and the technical advances permitting multiparameter quantification. The chapter hereby provides a detailed description of two-dimensional and three-dimensional approaches of cell dispersion and migration and finally gives a brief insight on computational quantification of the cell behavior.
authors.affiliation: INSERM, UMRS 903, Reims, France.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Bonnomet
authors.forename: Christine
authors.initials: C
authors.lastname: Terryn
authors.forename: Jérôme
authors.initials: J
authors.lastname: Cutrona
authors.forename: Antoine
authors.initials: A
authors.lastname: Jonquet
authors.forename: Philippe
authors.initials: P
authors.lastname: Birembaut
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Zahm
mesh_terms: D002450:Cell Communication; D045744:Cell Line, Tumor; D002465:Cell Movement; D058948:Cell Tracking / Q000379:methods*; D004847:Epithelial Cells / Q000166:cytology*; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D018715:Microscopy, Video / Q000295:instrumentation / Q000379:methods*; D009361:Neoplasm Invasiveness / Q000473:pathology; D046509:Tissue Culture Techniques / Q000379:methods*; D014945:Wound Healing