title: Quantitative trait locus mapping of yield-related components and oligogenic control of the cap color of the button mushroom, Agaricus bisporus.
abstract: As in other crops, yield is an important trait to be selected for in edible mushrooms, but its inheritance is poorly understood. Therefore, we have investigated the complex genetic architecture of yield-related traits in Agaricus bisporus through the mapping of quantitative trait loci (QTL), using second-generation hybrid progeny derived from a cross between a wild strain and a commercial cultivar. Yield, average weight per mushroom, number of fruiting bodies per m(2), earliness, and cap color were evaluated in two independent experiments. A total of 23 QTL were detected for 7 yield-related traits. These QTL together explained between 21% (two-flushes yield) and 59% (earliness) of the phenotypic variation. Fifteen QTL (65%) were consistent between the two experiments. Four regions underlying significant QTL controlling yield, average weight, and number were detected on linkage groups II, III, IV, and X, suggesting a pleiotropic effect or tight linkage. Up to six QTL were identified for earliness. The PPC1 locus, together with two additional genomic regions, explained up to 90% of the phenotypic variation of the cap color. Alleles from the wild parent showed beneficial effects for some yield traits, suggesting that the wild germ plasm is a valuable source of variation for several agronomic traits. Our results constitute a key step toward marker-assisted selection and provide a solid foundation to go further into the biological mechanisms controlling productive traits in the button mushroom.
authors.affiliation: INRA, Mycologie et Sécurité des Aliments, Villenave d’Ornon, France. mfoulong@bordeaux.inra.fr
authors.forename: Marie
authors.initials: M
authors.lastname: Foulongne-Oriol
authors.forename: Anne
authors.initials: A
authors.lastname: Rodier
authors.forename: Thierry
authors.initials: T
authors.lastname: Rousseau
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Savoie
mesh_terms: D000364:Agaricus / Q000235:genetics* / Q000502:physiology; D000383:Agriculture / Q000379:methods; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D003433:Crosses, Genetic; D005800:Genes, Fungal; D008040:Genetic Linkage; D010641:Phenotype; D010858:Pigmentation / Q000235:genetics*; D040641:Quantitative Trait Loci