title: Cloning and sequence analysis of complete gene encoding an alkaline lipase from Penicillium cyclopium.
abstract: The complete gene (PG37 lipI) encoding an alkaline lipase (PG37 LipI) was cloned from the genomic DNA of Penicillium cyclopium PG37. The cloned PG37 lipI is 2020 bp in length, consisting of 632 bp of the 5' flanking promoter region and 1388 bp of the downstream fragment that contains 6 exons and 5 short introns. The promoter region harbors putative TATA box, CAAT box and several transcription factor binding sites. The open reading frame (ORF) encodes a PG37 LipI of 285 amino acid residues, which was predicted to contain a 20-aa signal peptide, a 7-aa propeptide and a 258-aa mature peptide with a conserved motif Gly-X-Ser-X-Gly. However, PG37 LipI shows only 32%, 30%, 28% and 26% identity with lipases of Aspergillus parasiticus, Penicillium camembertii, Thermomyces lanuginosus and Rhizomucor miehei, respectively. It was predicted that the main secondary structures of PG37 LipI are alpha-helix and random coil. Three amino acid residues, Ser132-Asp188-His241, compose the enzymatic active center in the tertiary structure.
authors.affiliation: School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China.
authors.forename: H M
authors.initials: HM
authors.lastname: Zhang
authors.forename: M C
authors.initials: MC
authors.lastname: Wu
authors.forename: J
authors.initials: J
authors.lastname: Guo
authors.forename: J F
authors.initials: JF
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D020134:Catalytic Domain; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004926:Escherichia coli; D005091:Exons; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007218:Industrial Microbiology / Q000379:methods*; D007438:Introns; D008049:Lipase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010407:Penicillium / Q000201:enzymology* / Q000235:genetics; D011401:Promoter Regions, Genetic; D021382:Protein Sorting Signals; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D005656:Fungal Proteins; D021382:Protein Sorting Signals; D011994:Recombinant Proteins; D008049:Lipase