title: Preparation, characterization and anticoagulant activity in vitro of heparin-like 6-carboxylchitin derivative.
abstract: A series of heparin-like 6-carboxylchitin derivatives with different N-acetyl group and sulfate group contents were prepared. Their structures were characterized by element analysis, FT-IR, (13)C NMR, and gel permeation chromatography. Their anticoagulant activity in vitro was investigated for human plasma with respect to activated partial thromboplastin time (APTT). The results showed all 6-carboxylchitin derivatives prolonged APTT within the scope of studied degree of sulfation (0.28-1.03) and Mws (4.3-13.7 kDa). Their anticoagulant activity strongly depended on their structures. 3,6-O-sulfated group promoted the anticoagulant activity. Only incorporation of N-sulfated group into deacetylated 6-carboxylchitin could not improve the anticoagulant activity. But, N-sulfated group and O-sulfated group had the synergistic action, and N-sulfated group could promote the anticoagulant activity for the N,O-sulfated chitin derivatives. In addition, acetyl group took a role in the anticoagulant activity, too.
authors.affiliation: Department of Environmental Engineering, School of Environmental and Safety Engineering, Changzhou University, Changzhou, China. yangshenyang3@163.com
authors.forename: Jianhong
authors.initials: J
authors.lastname: Yang
authors.forename: Jun
authors.initials: J
authors.lastname: Cai
authors.forename: Kang
authors.initials: K
authors.lastname: Wu
authors.forename: Dinglong
authors.initials: D
authors.lastname: Li
authors.forename: Ying
authors.initials: Y
authors.lastname: Hu
authors.forename: Guoxiang
authors.initials: G
authors.lastname: Li
authors.forename: Yumin
authors.initials: Y
authors.lastname: Du
mesh_terms: D000107:Acetylation; D000925:Anticoagulants / Q000737:chemistry* / Q000494:pharmacology*; D040761:Biomimetic Materials / Q000737:chemistry* / Q000494:pharmacology*; D002686:Chitin / Q000737:chemistry* / Q000494:pharmacology*; D006493:Heparin / Q000737:chemistry*; D006801:Humans; D010314:Partial Thromboplastin Time; D013431:Sulfates / Q000737:chemistry
chemical_list: D000925:Anticoagulants; D013431:Sulfates; D002686:Chitin; D006493:Heparin