title: Sensitive sandwich ELISA based on a gold nanoparticle layer for cancer detection.
abstract: The availability of techniques for the sensitive detection of early stage cancer is crucial for patient survival. Our previous research (Langmuir, 2011, 27, 2155-2158) showed that gold nanoparticle layers (GNPL) used in indirect format ELISA amplified the signal, and gave a lower limit of detection (LOD) compared with commercial ELISA plates. However, due to its intrinsic limitations, indirect ELISA is not suitable for samples of complex composition, such as serum, plasma, etc., thus limiting the clinical performance of this kind of ELISA. In the work reported here, a GNPL-based sandwich format ELISA was developed, which showed superiority in terms of detection limit and sensitivity in the determination of rabbit IgG in buffer. More importantly, experiments using plasma spiked with carcinoembryonic antigen (CEA) as a representative biomarker showed that our GNPL-based ELISA assay amplified the signal and lowered the LOD compared to other assays, including commercialized CEA ELISA kits. This simple and cost-effective GNPL-based sandwich ELISA holds promise in clinical applications.
authors.affiliation: Jiangsu Key Laboratory of Advanced Functional Polymer Design and Application, Department of Polymer Science and Engineering, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou, 215123, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhou
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Wang
authors.forename: Lin
authors.initials: L
authors.lastname: Yuan
authors.forename: Zhenping
authors.initials: Z
authors.lastname: Cheng
authors.forename: Zhaoqiang
authors.initials: Z
authors.lastname: Wu
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
mesh_terms: D002272:Carcinoembryonic Antigen / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D053768:Metal Nanoparticles; D009369:Neoplasms / Q000175:diagnosis*
chemical_list: D002272:Carcinoembryonic Antigen; D006046:Gold