title: [Time effect of sinusoidal electromagnetic field on enhancing the maturation and mineralization of osteoblasts in vitro].
abstract: The present research was to investigate the time effect of sinusoidal electromagnetic fields (SEMFs) at different exposure time on the proliferation and differentiation of osteoblasts (OB) in vitro. The newborn rat calvarial OB were isolated by enzyme digestion and divided randomly into 7 groups after one passage. The exposure times of the SEMFs were 0.5 h, 1.0 h, 1.5 h, 2.0 h, 2.5 h and 3.0 h, respectively, and the frequency was 50 Hz. The cells were exposed in the SEMFs of 1.8 mT. Those without SEMFs exposure were used as the control group. They were observed under the contrast phase microscope each day. After 48 h, cell proliferation was assayed by MTT method. The alkaline phosphatase (Alkaline Phosphatase, ALP) activities were measured after the exposure of SEMFs for 3 d, 6 d, 9 d and 12 d, respectively. The calcified nodules were stained by Alizarin Bordeaux after 10 d. The cells exposed in the SEMFs were arranged in Spiral appearance after 8 d. The SEMFs exposure time at 2.0 h, 2.5 h and 3.0 h significantly inhibited cell proliferation (P < 0.01) and 0.5 h, 1.0 h, 1.5 h groups more significantly than control groups (P < 0.05). When the 3 d, 6 d and 12 d the ALP activities of the 0.5 h, 1.0 h, 1.5 h and 2.0 h, times group were significantly higher than those in the control group (P < 0.05), and after 9 d the 1.0 h, 1.5 h and 2.0 h activity of ALP higher significantly than control and other groups (P < 0.01). Other groups had no effect on the ALP activity. Alizarin Bordeaux staining result showed the amounts of calcified nodules 1.0 h, 1.5 h and 2.0 h higher than control groups. The SEMFs at 50 Hz, 1.8 mT different time exposure groups inhibits the proliferation of OB, but they enhances the maturation and mineralization of the OB and SEMFs at 1.8 mT of the 1.5 h has the strongest activity.
authors.affiliation: Institute of Orthopaedics, Lanzhou General Hospital, Lanzhou 730050, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhou
authors.forename: Baofeng
authors.initials: B
authors.lastname: Ge
authors.forename: Keming
authors.initials: K
authors.lastname: Chen
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Li
authors.forename: Guozheng
authors.initials: G
authors.lastname: Cheng
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Wang
authors.forename: Leiguo
authors.initials: L
authors.lastname: Ming
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wei
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002113:Calcification, Physiologic / Q000528:radiation effects*; D002454:Cell Differentiation / Q000528:radiation effects*; D049109:Cell Proliferation / Q000528:radiation effects; D002478:Cells, Cultured; D004574:Electromagnetic Fields; D010006:Osteoblasts / Q000166:cytology*; D010012:Osteogenesis; D051381:Rats; D017207:Rats, Sprague-Dawley