title: Polar electrophoresis: shape of two-dimensional maps is as important as size.
abstract: The performance of two-dimensional electrophoresis in conventional gels in Cartesian coordinates (2-DE) vs. polar coordinates (2-PE) is here evaluated. Although 2-DE is performed in much longer Immobiline gels in the first dimension (17 cm) vs. barely 7-cm in 2-PE, an equivalent resolving power is found. Moreover, due to the possibility of running up to seven Immobiline strips in the radial gel format, the reproducibility of spot position is seen to be higher, this resulting in a 20% higher matching efficiency. As an extra bonus, strings of "isobaric" spots (i.e. polypeptides of identical mass with different pI values) are more resolved in the radial gel format, especially in the 10 to 30 kDa region, where the gel area fans out leaving extra space for spot resolution. In conclusion, this novel gel format in the second dimension of 2D gels is seen as an important improvement of this technique, still one of the most popular in proteome analysis.
authors.affiliation: Department of Medicine, University of Padova, Padova, Italy.
authors.forename: Renato
authors.initials: R
authors.lastname: Millioni
authors.forename: Rita
authors.initials: R
authors.lastname: Polati
authors.forename: Michele
authors.initials: M
authors.lastname: Menini
authors.forename: Lucia
authors.initials: L
authors.lastname: Puricelli
authors.forename: Manuela
authors.initials: M
authors.lastname: Miuzzo
authors.forename: Paolo
authors.initials: P
authors.lastname: Tessari
authors.forename: Enrico
authors.initials: E
authors.lastname: Novelli
authors.forename: Pier Giorgio
authors.initials: PG
authors.lastname: Righetti
authors.forename: Daniela
authors.initials: D
authors.lastname: Cecconi
mesh_terms: D000818:Animals; D002417:Cattle; D015180:Electrophoresis, Gel, Two-Dimensional / Q000295:instrumentation / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D007089:Image Enhancement / Q000295:instrumentation / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000295:instrumentation / Q000379:methods*; D008962:Models, Theoretical; D009124:Muscle Proteins / Q000032:analysis / Q000378:metabolism; D010449:Peptide Mapping / Q000295:instrumentation / Q000379:methods*; D020543:Proteome / Q000032:analysis / Q000378:metabolism; D040901:Proteomics / Q000295:instrumentation* / Q000379:methods*
chemical_list: D009124:Muscle Proteins; D020543:Proteome