title: MicroRNA-195-5p suppresses glucose uptake and proliferation of human bladder cancer T24 cells by regulating GLUT3 expression.
abstract: It has been reported that expression of glucose transporter member 3 (GLUT3) is up-regulated in bladder cancers. However, the regulating mechanism remains unknown. Here, we assessed whether microRNAs (miRNAs) regulate GLUT3 expression in bladder cancers. In our study, miR-195-5p was identified to directly targeted GLUT3 3'-untranslated region (UTR) in bladder cancer T24 cells. Small interfering RNA (siRNA)- and miR-195-5p-mediated GLUT3 knockdown experiments revealed that miR-195-5p decreased T24 cells glucose uptake, inhibited cell growth and promoted cell apoptosis through suppression of GLUT3 expression. Therefore, miR-195-5p is a novel and also the first identified miRNA that targets GLUT3, and the aberrant decreased expression of miR-195-5p and consequent GLUT3 up-regulation may contribute to bladder carcinogenesis.
authors.affiliation: Department of Urology, the Affiliated Shengjing Hospital, China Medical University, Shenyang, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Fei
authors.forename: Manlong
authors.initials: M
authors.lastname: Qi
authors.forename: Bin
authors.initials: B
authors.lastname: Wu
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Song
authors.forename: Yueping
authors.initials: Y
authors.lastname: Wang
authors.forename: Tingting
authors.initials: T
authors.lastname: Li
mesh_terms: D020413:3' Untranslated Regions; D017209:Apoptosis / Q000235:genetics; D001483:Base Sequence; D001693:Biological Transport, Active; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D005947:Glucose / Q000378:metabolism*; D051274:Glucose Transporter Type 3 / Q000235:genetics*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology
chemical_list: D020413:3' Untranslated Regions; D051274:Glucose Transporter Type 3; C552722:MIRN195 microRNA, human; D035683:MicroRNAs; D034741:RNA, Small Interfering; C495687:SLC2A3 protein, human; D005947:Glucose