title: Increased furan tolerance in Escherichia coli due to a cryptic ucpA gene.
abstract: Expression arrays were used to identify 4 putative oxidoreductases that were upregulated (>3-fold) by furfural (15 mM, 15 min). Plasmid expression of one (ucpA) increased furan tolerance in ethanologenic strain LY180 and wild-type strain W. Deleting ucpA decreased furfural tolerance. Although the mechanism remains unknown, the cryptic ucpA gene is now associated with a phenotype: furan resistance.
authors.affiliation: Department of Microbiology and Cell Science, University of Florida, Gainesville, Florida, USA.
authors.forename: Xuan
authors.initials: X
authors.lastname: Wang
authors.forename: Elliot N
authors.initials: EN
authors.lastname: Miller
authors.forename: Lorraine P
authors.initials: LP
authors.lastname: Yomano
authors.forename: K T
authors.initials: KT
authors.lastname: Shanmugam
authors.forename: Lonnie O
authors.initials: LO
authors.lastname: Ingram
mesh_terms: D000429:Alcohol Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D003470:Culture Media; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D005662:Furaldehyde / Q000378:metabolism / Q000494:pharmacology*; D005663:Furans / Q000378:metabolism / Q000494:pharmacology*; D015964:Gene Expression Regulation, Bacterial; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009243:NAD / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D015854:Up-Regulation; D014994:Xylose / Q000378:metabolism
chemical_list: D003470:Culture Media; D029968:Escherichia coli Proteins; D005663:Furans; D009243:NAD; D000431:Ethanol; D014994:Xylose; D005662:Furaldehyde; D000429:Alcohol Oxidoreductases; C039281:furan