title: Magnetic pH-responsive nanogels as multifunctional delivery tools for small interfering RNA (siRNA) molecules and iron oxide nanoparticles (IONPs).
abstract: We here exploit pH-responsive nanogels as carriers to deliver functional anti-GFP siRNA and superparamagnetic IONPs to HeLa-GFP cells. The siRNA release via pH-mediated endosomal escape is shown. The IONPs act first as magnetofection agents to boost cellular uptake and then as probes to track the release mechanism by electron microscopy.
authors.affiliation: Istituto Italiano di Tecnologia, via Morego 30, 161613, Genoa, Italy.
authors.forename: Alberto
authors.initials: A
authors.lastname: Curcio
authors.forename: Roberto
authors.initials: R
authors.lastname: Marotta
authors.forename: Andreas
authors.initials: A
authors.lastname: Riedinger
authors.forename: Domenico
authors.initials: D
authors.lastname: Palumberi
authors.forename: Andrea
authors.initials: A
authors.lastname: Falqui
authors.forename: Teresa
authors.initials: T
authors.lastname: Pellegrino
mesh_terms: D001692:Biological Transport; D004337:Drug Carriers / Q000737:chemistry*; D005290:Ferric Compounds / Q000737:chemistry* / Q000378:metabolism*; D005782:Gels; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D042541:Intracellular Space / Q000378:metabolism; D060328:Magnetic Phenomena; D053758:Nanoparticles / Q000737:chemistry*; D034741:RNA, Small Interfering / Q000378:metabolism*
chemical_list: D004337:Drug Carriers; D005290:Ferric Compounds; D005782:Gels; D034741:RNA, Small Interfering; C000499:ferric oxide