title: A novel murine model for the in vivo study of transdermal drug penetration.
abstract: Enhancement of the transdermal penetration of different active agents is an important research goal. Our aim was to establish a novel in vivo experimental model which provides a possibility for exact measurement of the quantity of penetrated drug. The experiments were performed on SKH-1 hairless mice. A skin fold in the dorsal region was fixed with two fenestrated titanium plates. A circular wound was made on one side of the skin fold. A metal cylinder with phosphate buffer was fixed into the window of the titanium plate. The concentration of penetrated drug was measured in the buffer. The skin fold was morphologically intact and had a healthy microcirculation. The drug appeared in the acceptor buffer after 30 min, and its concentration exhibited a continuous increase. The presence of ibuprofen was also detected in the plasma. In conclusion, this model allows an exact in vivo study of drug penetration and absorption.
authors.affiliation: Department of Dermatology and Allergology, University of Szeged, 6720 Szeged, Hungary. erosg@expsur.szote.u-szeged.hu
authors.forename: Gábor
authors.initials: G
authors.lastname: Eros
authors.forename: Petra
authors.initials: P
authors.lastname: Hartmann
authors.forename: Szilvia
authors.initials: S
authors.lastname: Berkó
authors.forename: Eszter
authors.initials: E
authors.lastname: Csizmazia
authors.forename: Erzsébet
authors.initials: E
authors.lastname: Csányi
authors.forename: Anita
authors.initials: A
authors.lastname: Sztojkov-Ivanov
authors.forename: István
authors.initials: I
authors.lastname: Németh
authors.forename: Piroska
authors.initials: P
authors.lastname: Szabó-Révész
authors.forename: István
authors.initials: I
authors.lastname: Zupkó
authors.forename: Lajos
authors.initials: L
authors.lastname: Kemény
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D007052:Ibuprofen / Q000008:administration & dosage / Q000493:pharmacokinetics; D008297:Male; D051379:Mice; D008812:Mice, Hairless / Q000378:metabolism*; D008833:Microcirculation / Q000187:drug effects; D008856:Microscopy, Fluorescence; D012867:Skin / Q000033:anatomy & histology / Q000098:blood supply / Q000187:drug effects / Q000378:metabolism; D012869:Skin Absorption / Q000187:drug effects
chemical_list: D007052:Ibuprofen