title: Secretory leukocyte protease inhibitor (SLPI) might contaminate murine monoclonal antibodies after purification on protein G.
abstract: The large scale production of a monoclonal anti-progesterone antibody in serum free medium followed by affinity chromatography on protein G lead to a contamination of the antibody sample with a protein of about 14 kDa. This protein was identified by mass spectrometry as secretory leukocyte protease inhibitor (SLPI). This SLPI contamination lead to a failure of the fiber-optic based competitive fluorescence assay to detect progesterone in milk. Purification of the monoclonal antibody using protein A columns circumvented this problem.
authors.affiliation: UP Transfer GmbH, Am Neuen Palais 10, 14469 Potsdam, Germany. joerg.schenk@up-transfer.de
authors.forename: Jörg A
authors.initials: JA
authors.lastname: Schenk
authors.forename: Joerg
authors.initials: J
authors.lastname: Fettke
authors.forename: Christine
authors.initials: C
authors.lastname: Lenz
authors.forename: Katharina
authors.initials: K
authors.lastname: Albers
authors.forename: Frank
authors.initials: F
authors.lastname: Mallwitz
authors.forename: Nenad
authors.initials: N
authors.lastname: Gajovic-Eichelmann
authors.forename: Eva
authors.initials: E
authors.lastname: Ehrentreich-Förster
authors.forename: Emely
authors.initials: E
authors.lastname: Kusch
authors.forename: Frank
authors.initials: F
authors.lastname: Sellrie
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology / Q000302:isolation & purification*; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000737:chemistry; D002846:Chromatography, Affinity / Q000379:methods; D051379:Mice; D008892:Milk / Q000737:chemistry; D011374:Progesterone / Q000276:immunology*; D053481:Secretory Leukocyte Peptidase Inhibitor / Q000737:chemistry / Q000302:isolation & purification*; D013205:Staphylococcal Protein A / Q000737:chemistry
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; C034284:IgG Fc-binding protein, Streptococcus; D053481:Secretory Leukocyte Peptidase Inhibitor; D013205:Staphylococcal Protein A; D011374:Progesterone