title: Determination of SCFAs in water using GC-FID. Selection of the separation system.
abstract: In the present study, an analytical procedure was developed for the determination of short-chain fatty acids (SCFAs) in landfill leachate and municipal wastewater employing injection of aqueous samples to gas chromatograph with flame ionization detector (GC-FID). Chromatographic conditions such as a separation system, injection volume, oven temperature program were investigated and selected. With two columns, one with a polar (polyethylene glycol) and one with a non-polar (dimethylpolisiloxane) stationary phase, good separation of SCFAs, containing from 2 to 8 carbon atoms, was achieved. The sample volume was 2 μL and the temperature program 80°C (30 s) then 7 °C min(-1) to 220 °C (2 min). LOQs values were below 0.25 mg L(-1). The concentrations of the acids in the landfill leachate studied ranged from 0.45±0,059 (average ± extended uncertainty) mg L(-1) for pentanoic acid to 15.2±0.73 mg L(-1) for ethanoic acid. Concentrations of SCFAs in the municipal wastewater were lower than LOQs.
authors.affiliation: Department of Analytical Chemistry, Chemical Faculty, Gdańsk University of Technology (GUT), Gdańsk, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Banel
authors.forename: Anna
authors.initials: A
authors.lastname: Jakimska
authors.forename: Marta
authors.initials: M
authors.lastname: Wasielewska
authors.forename: Lidia
authors.initials: L
authors.lastname: Wolska
authors.forename: Bogdan
authors.initials: B
authors.lastname: Zygmunt
mesh_terms: D004129:Dimethylpolysiloxanes / Q000737:chemistry; D005232:Fatty Acids, Volatile / Q000032:analysis* / Q000302:isolation & purification; D005410:Flame Ionization; D011092:Polyethylene Glycols / Q000737:chemistry; D013696:Temperature; D014865:Waste Disposal, Fluid; D014867:Water / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000737:chemistry
chemical_list: D004129:Dimethylpolysiloxanes; D005232:Fatty Acids, Volatile; D014874:Water Pollutants, Chemical; D014867:Water; D011092:Polyethylene Glycols