title: Functional cloning and characterization of antibiotic resistance genes from the chicken gut microbiome.
abstract: Culture-independent sampling in conjunction with a functional cloning approach identified diverse antibiotic resistance genes for different classes of antibiotics in gut microbiomes from both conventionally raised and free-range chickens. Many of the genes are phylogenetically distant from known resistance genes. Two unique genes that conferred ampicillin and spectinomycin resistance were also functional in Campylobacter, a distant relative of the Escherichia coli host used to generate the genomic libraries.
authors.affiliation: Department of Animal Science, The University of Tennessee, Knoxville, Tennessee, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Lin
mesh_terms: D000818:Animals; D001419:Bacteria / Q000187:drug effects* / Q000235:genetics*; D002645:Chickens; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D024881:Drug Resistance, Bacterial; D041981:Gastrointestinal Tract / Q000382:microbiology*; D005798:Genes, Bacterial; D014644:Genetic Variation; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial