title: Multiplexed microneedle-based biosensor array for characterization of metabolic acidosis.
abstract: The development of a microneedle-based biosensor array for multiplexed in situ detection of exercise-induced metabolic acidosis, tumor microenvironment, and other variations in tissue chemistry is described. Simultaneous and selective amperometric detection of pH, glucose, and lactate over a range of physiologically relevant concentrations in complex media is demonstrated. Furthermore, materials modified with a cell-resistant (Lipidure(®)) coating were shown to inhibit macrophage adhesion; no signs of coating delamination were noted over a 48-h period.
authors.affiliation: Joint Department of Biomedical Engineering, University of North Carolina and North Carolina State University, Raleigh, NC 27695-7115, USA.
authors.forename: Philip R
authors.initials: PR
authors.lastname: Miller
authors.forename: Shelby A
authors.initials: SA
authors.lastname: Skoog
authors.forename: Thayne L
authors.initials: TL
authors.lastname: Edwards
authors.forename: Deanna M
authors.initials: DM
authors.lastname: Lopez
authors.forename: David R
authors.initials: DR
authors.lastname: Wheeler
authors.forename: Dulce C
authors.initials: DC
authors.lastname: Arango
authors.forename: Xiaoyin
authors.initials: X
authors.lastname: Xiao
authors.forename: Susan M
authors.initials: SM
authors.lastname: Brozik
authors.forename: Joseph
authors.initials: J
authors.lastname: Wang
authors.forename: Ronen
authors.initials: R
authors.lastname: Polsky
authors.forename: Roger J
authors.initials: RJ
authors.lastname: Narayan
mesh_terms: D000138:Acidosis / Q000175:diagnosis / Q000378:metabolism*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002244:Carbon; D002448:Cell Adhesion; D020099:Coated Materials, Biocompatible; D055664:Electrochemical Techniques; D015444:Exercise; D005947:Glucose / Q000378:metabolism*; D005949:Glucose Oxidase / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000032:analysis* / Q000096:biosynthesis; D008264:Macrophages / Q000502:physiology; D008839:Microelectrodes; D009339:Needles
chemical_list: D020099:Coated Materials, Biocompatible; D019344:Lactic Acid; D002244:Carbon; D005949:Glucose Oxidase; D005947:Glucose