title: [In vitro antifungal susceptibility, in vivo antifungal activity and security from a natural product obtained from sunrise oil (AMO3) against dermatophytes].
abstract: This work studied safety and antifungal activity of ozonized sunflower oil (AMO3) against dermatophytes. AMO3 was prepared through a new original process that modifies the oil before ozonation by alcoholic catalytic esterification. Susceptibility was studied in 41 dermatophytes by agar diffusion and broth microdilution tests. The experimental model to assess the topical safety of the oil included 60 CF1 mice divided in three groups that were treated with vaseline (control), 1% AMO3 and 50% AMO3 (overdose), respectively. Then, experimental dermatophytosis was induced in CF1 mice. Seventy-five individuals were selected and divided in 5 groups that were treated once a day with placebo, cream with 1%, 2% and 3% AMO3 plus an untreated control group. This new natural product showed antifungal activity against all strains studied. The MIC ranged between was 0,125 and 1%, while minimum fungicidal concentration (MFC) was 2%. The application of vaseline and AMO3 1% and 50% did not produce clinical or histopathological lesions. The mice with dermatophytosis that were treated with 1%, 2% and 3% AMO3 showed 100% clinical cure and 94% average mycological cure, exceeding placebo and control groups (p < 0,05). This product exhibits high antifungal activity and could be a safe alternative for ringworm topical treatment.
authors.affiliation: Facultad de Medicina, Universidad Mayor Santiago, Chile.
authors.forename: Pamela
authors.initials: P
authors.lastname: Thomson M
authors.forename: Sonia
authors.initials: S
authors.lastname: Anticevic C
authors.forename: Héctor
authors.initials: H
authors.lastname: Rodríguez B
authors.forename: Víctor
authors.initials: V
authors.lastname: Silva V
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology* / Q000627:therapeutic use; D003883:Arthrodermataceae / Q000187:drug effects*; D051379:Mice; D008826:Microbial Sensitivity Tests / Q000379:methods; D010938:Plant Oils / Q000494:pharmacology* / Q000627:therapeutic use; D000074242:Sunflower Oil; D014005:Tinea / Q000188:drug therapy* / Q000382:microbiology / Q000473:pathology
chemical_list: D000935:Antifungal Agents; D010938:Plant Oils; D000074242:Sunflower Oil