title: Selective Expansion of CD34+ Cells from Mouse Bone Marrow Cultured on LH/P MP-Coated Plates with Adequate Cytokines.
abstract: Low-molecular-weight heparin/protamine microparticles (LH/P MPs) serve as carriers for controlled release of heparin-binding cytokines. LH/P MPs were stably coated onto plastic surfaces by drying. The purpose of this study is to evaluate a culture method for selective expansion of CD34+ cells using LH/P MPs as cytokine-binding matrix. Ficoll-purified mouse bone marrow cells (mouse FP-BMCs) containing CD34+ cells were cultured on LH/P MP-coated plates in the presence of stem cell factor (SCF), thrombopoietin (Tpo), and Flt-3 ligand (Flt-3) in hematopoietic progenitor growth medium (HPGM) supplemented with 4% heat-inactivated fetal bovine serum (FBS). After 8 days of culture, the total cell count increased 4.6-fold, and flow cytometry analyses revealed that 23.8% of the initial cells and 57.4% of the expanded cells were CD34 positive. Therefore, CD34+ cells were estimated to have increased 11.0-fold. In contrast, cultured CD34+ cells on uncoated tissue culture plates increased 5.8-fold in an identical medium.
authors.affiliation: Research Institute, National Defense Medical College, Tokorozawa, Saitama 359-8513, Japan.
authors.forename: Satoko
authors.initials: S
authors.lastname: Kishimoto
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ishihara
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kanatani
authors.forename: Masaki
authors.initials: M
authors.lastname: Nambu
authors.forename: Megumi
authors.initials: M
authors.lastname: Takikawa
authors.forename: Yuki
authors.initials: Y
authors.lastname: Sumi
authors.forename: Shingo
authors.initials: S
authors.lastname: Nakamura
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Mori
authors.forename: Hidemi
authors.initials: H
authors.lastname: Hattori
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Toshinori
authors.initials: T
authors.lastname: Sato