title: Pyrosequencing-based barcodes for a dye-free multiplex bioassay.
abstract: A novel dye-free labeling method for a multiplex bioassay was proposed by using short sequence-based barcodes consisting of a reporter base and repeats of two stuffer bases; then, the barcodes were quantitatively decoded by a single pyrosequencing assay without any pre-separation.
authors.affiliation: Department of Pharmacology, Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu 210002, PR China.
authors.forename: Zhiyao
authors.initials: Z
authors.lastname: Chen
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Fu
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Zhang
authors.forename: Xiqun
authors.initials: X
authors.lastname: Liu
authors.forename: Bingjie
authors.initials: B
authors.lastname: Zou
authors.forename: Haiping
authors.initials: H
authors.lastname: Wu
authors.forename: Qinxin
authors.initials: Q
authors.lastname: Song
authors.forename: Jinheng
authors.initials: J
authors.lastname: Li
authors.forename: Tomoharu
authors.initials: T
authors.lastname: Kajiyama
authors.forename: Hideki
authors.initials: H
authors.lastname: Kambara
authors.forename: Guohua
authors.initials: G
authors.lastname: Zhou
mesh_terms: D000199:Actins / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D001681:Biological Assay / Q000379:methods*; D051358:Cyclin-Dependent Kinase 4 / Q000235:genetics; D051379:Mice; D017421:Sequence Analysis / Q000379:methods*; D013194:Staining and Labeling
chemical_list: D000199:Actins; D051358:Cyclin-Dependent Kinase 4