title: [Dynamic changes of 5-HT, DA and their metabolin in rat striatum during exhaustive exercise and recovery].
abstract: OBJECTIVE
  To study the dynamic characteristics of serotonin (5-HT), dopamine (DA) and their metabolin changes in brain during the development of exercise-induced central fatigue.
  
  
  METHODS
  Coupling of microdialysis and capillary electrophoresis-laser induced fluorescence detection method were used to continuously monitored the changes of DA, tryptophan (Trp), tyrosine (Tyr), 5-HT and 5-hydroxyindoleacetic acid (5-HIAA) in striatum extracellular fluid during the exhaustive exercise and recovery time.
  
  
  RESULTS
  The concentrations of Trp, 5-HT, 5-HIAA in striatum extracellular fluid had no remarkable changes in the early time of exercise (P < 0.05), while they significantly increased during the later time of exercise and whole recovery time (P < 0.05, P < 0.01). The concentrations of DA and Tyr significantly increased over basal level in the later exercise time, exhaust and recovery time (P < 0.05, P < 0.01). DA/5-HT significantly increased in the initial time of exercise (P < 0.05, P < 0.01), while decreased during the later exercise time, the nadir occurred at 15 minutes before rats exhausted. DA/5-HT slightly recovered back to basal level during the recovery time, and there was no significant difference during later exercise, exhausted and recovery time compared with basal level (P < 0.05).
  
  
  CONCLUSIONS
  The changes of DA and 5-HT in striatum have phase characteristics. Both of them significantly increase during the development of exercise-induced fatigue. However, the 5-HT plays the dominant role in the dynamic changes of them.
authors.affiliation: College of Physical Education and Sports, Beijing Normal University, Beijing 100875, China.
authors.forename: Dong-Sheng
authors.initials: DS
authors.lastname: Yang
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Liu
authors.forename: De-Cai
authors.initials: DC
authors.lastname: Qiao
mesh_terms: D000818:Animals; D003342:Corpus Striatum / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism*; D005221:Fatigue / Q000378:metabolism / Q000503:physiopathology*; D008297:Male; D010805:Physical Conditioning, Animal / Q000502:physiology; D005082:Physical Exertion / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000378:metabolism*
chemical_list: D012701:Serotonin; D004298:Dopamine