title: Exercise as a model to study redox homeostasis in blood: the effect of protocol and sampling point.
abstract: Twenty males ran either on a level treadmill (nonmuscle-damaging condition) or on a downhill treadmill (muscle-damaging condition). Blood and urine samples were collected before and after exercise (immediately after, 1h, 4h, 24h, 48h, and 96h). The following assays were performed: F(2)-isoprostanes in urine, protein carbonyls in plasma, glutathione, superoxide dismutase, glutathione peroxidase, and catalase in erythrocytes. The main finding was that monophasic redox responses were detected after nonmuscle-damaging exercise compared to the biphasic responses detected after muscle-damaging exercise. Based on these findings, muscle-damaging exercise may be a more appropriate experimental model to induce physiological oxidative stress.
authors.affiliation: Exercise Physiology and Biochemistry Laboratory, Department of Physical Education and Sport Sciences at Serres, Aristotle University of Thessaloniki, Greece. nikolaidis@auth.gr
authors.forename: Michalis G
authors.initials: MG
authors.lastname: Nikolaidis
authors.forename: Antonios
authors.initials: A
authors.lastname: Kyparos
authors.forename: Konstantina
authors.initials: K
authors.lastname: Dipla
authors.forename: Andreas
authors.initials: A
authors.lastname: Zafeiridis
authors.forename: Michalis
authors.initials: M
authors.lastname: Sambanis
authors.forename: Gerasimos V
authors.initials: GV
authors.lastname: Grivas
authors.forename: Vassilis
authors.initials: V
authors.lastname: Paschalis
authors.forename: Anastasios A
authors.initials: AA
authors.lastname: Theodorou
authors.forename: Stavros
authors.initials: S
authors.lastname: Papadopoulos
authors.forename: Chrysoula
authors.initials: C
authors.lastname: Spanou
authors.forename: Ioannis S
authors.initials: IS
authors.lastname: Vrabas
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood / Q000652:urine; D002374:Catalase / Q000097:blood; D004912:Erythrocytes / Q000737:chemistry; D015444:Exercise; D005080:Exercise Test; D028441:F2-Isoprostanes / Q000652:urine; D005978:Glutathione / Q000097:blood; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D008297:Male; D008954:Models, Biological; D018482:Muscle, Skeletal / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology*; D050050:Protein Carbonylation; D012420:Running; D013482:Superoxide Dismutase / Q000097:blood; D013997:Time Factors
chemical_list: D015415:Biomarkers; D028441:F2-Isoprostanes; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione