title: Recombinant dengue type 2 viruses with altered e protein domain III epitopes are efficiently neutralized by human immune sera.
abstract: Humans develop polyclonal, serotype-specific neutralizing antibody responses after dengue virus (DENV) infection. Many mouse antibodies that neutralize DENV bind to the lateral ridge or A strand epitopes on domain III of the viral envelope (EDIII) protein. It has been assumed that these epitopes are also the main target of human neutralizing antibodies. Using recombinant dengue serotype 2 viruses with altered EDIII epitopes, we demonstrate that EDIII epitopes are not the main target of human neutralizing antibody.
authors.affiliation: Address correspondence to Aravinda M. de Silva, desilva@med.unc.edu.
authors.forename: Wahala M P B
authors.initials: WM
authors.lastname: Wahala
authors.forename: Claire
authors.initials: C
authors.lastname: Huang
authors.forename: Siritorn
authors.initials: S
authors.lastname: Butrapet
authors.forename: Laura J
authors.initials: LJ
authors.lastname: White
authors.forename: Aravinda M
authors.initials: AM
authors.lastname: de Silva
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology; D003715:Dengue / Q000276:immunology* / Q000821:virology; D003716:Dengue Virus / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D000939:Epitopes / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007106:Immune Sera / Q000276:immunology*; D051379:Mice; D017434:Protein Structure, Tertiary; D014759:Viral Envelope Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D000939:Epitopes; D007106:Immune Sera; D014759:Viral Envelope Proteins