title: Method validation and determinations of levofloxacin, metronidazole and sulfamethoxazole in an aqueous pharmaceutical, urine and blood plasma samples using quantitative nuclear magnetic resonance spectrometry.
abstract: Selective, rapid and accurate quantitative proton nuclear magnetic resonance (qHNMR) method for the determination of levofloxacin, metronidazole benzoate and sulfamethoxazole in aqueous solutions was developed and validated. The method was successfully applied to the determinations of the drugs and their admixtures in pharmaceutical, urine and plasma samples. Maleic acid and sodium malate were used as internal standards. Effect of temperature on spectral measurements was evaluated. Linear dynamic ranges of 0.50-68.00, 0.13-11.30 and 0.24-21.00 mg per 0.60 mL solution were obtained for levofloxacin, metronidazole benzoate and sulfamethoxazole, respectively. Average recovery % in the range of 96.00-104.20 ± (0.17-2.91) was obtained for drugs in pure, pharmaceutical, plasma and urine samples. Inter and intra-day analyses gave average recoveries % in the ranges 96.10-98.40 ± (1.68-2.81) and 96.00-104.20 ± (0.17-2.91), respectively. Instrumental detection limits ≤0.03 mg per 0.6 mL were obtained for the three drugs. Developed method has demonstrated high performance characteristics for analyzing investigated drugs and their admixtures. Student t-test at 95% confidence level revealed insignificant bias between the real and measured contents of investigated drugs in pure, pharmaceutical, urine and plasma samples and its admixtures. Application of the statistical F-test revealed insignificant differences in precisions between the developed method and arbitrary selected reference methods.
authors.affiliation: Department of Chemistry, Faculty of Science, United Arab Emirates University, Al-Ain, P.O. Box 17551, United Arab Emirates. asalem@uaeu.ac.ae
authors.forename: Alaa A
authors.initials: AA
authors.lastname: Salem
authors.forename: Hussein A
authors.initials: HA
authors.lastname: Mossa
mesh_terms: D006801:Humans; D064704:Levofloxacin; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008298:Maleates / Q000032:analysis; D008795:Metronidazole / Q000097:blood / Q000652:urine; D015242:Ofloxacin / Q000097:blood / Q000652:urine; D012680:Sensitivity and Specificity; D013420:Sulfamethoxazole / Q000097:blood / Q000652:urine; D013696:Temperature
chemical_list: D008298:Maleates; D008795:Metronidazole; D064704:Levofloxacin; C030272:maleic acid; D015242:Ofloxacin; D013420:Sulfamethoxazole