title: Labdane-type diterpenoids from the rhizomes of Hedychium coronarium inhibit lipopolysaccharide-stimulated production of pro-inflammatory cytokines in bone marrow-derived dendritic cells.
abstract: The rhizomes of Hedychium coronarium have been used for the treatment of inflammation, skin diseases, headache, and sharp pain due to rheumatism in traditional medicine. From this plant, two new labdanes, 15-methoxylabda-8(17),11E,13-trien-16,15-olide (1) and 16-methoxylabda-8(17),11E,13-trien-15,16-olide (3), named hedycoronens A and B, as well as four known, labda-8(17),11,13-trien-16,15-olide (2), 16-hydroxylabda-8(17),11,13-trien-15,16-olide (4), coronarin A (5), and corronarin E (6) were isolated. Their chemical structures were elucidated by mass, 1D- and 2D-nuclear magnetic resonance (NMR) spectroscopy. They were evaluated for inhibitory effects on the lipopolysaccharide (LPS)-stimulated production of pro-inflammatory cytokines in bone marrow-derived dendritic cells. Among of them, compounds 1-3 were potent inhibitors of LPS-stimulated interleukin-6 (IL-6) and IL-12 p40, with IC(50) ranging from 4.1±0.2 to 9.1±0.3 μM. Compounds 1 and 3 showed moderate inhibitory activity on the tumor necrosis factor-α (TNF-α) production with IC(50) values of 46.0±1.3 and 12.7±0.3 μM. The remains of compounds showed inactivity. These results warrant further studies concerning the potential anti-inflammatory benefits of labdane-diterpenes from H. coronarium.
authors.affiliation: Institute of Marine Biochemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Caugiay, Hanoi, Vietnam.
authors.forename: Phan Van
authors.initials: PV
authors.lastname: Kiem
authors.forename: Hoang Le Tuan
authors.initials: Hle T
authors.lastname: Anh
authors.forename: Nguyen Xuan
authors.initials: NX
authors.lastname: Nhiem
authors.forename: Chau Van
authors.initials: CV
authors.lastname: Minh
authors.forename: Nguyen Thi Kim
authors.initials: NT
authors.lastname: Thuy
authors.forename: Pham Hai
authors.initials: PH
authors.lastname: Yen
authors.forename: Dan Thuy
authors.initials: DT
authors.lastname: Hang
authors.forename: Bui Huu
authors.initials: BH
authors.lastname: Tai
authors.forename: Vivek Bhakta
authors.initials: VB
authors.lastname: Mathema
authors.forename: Young-Sang
authors.initials: YS
authors.lastname: Koh
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Kim
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism*; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects*; D004224:Diterpenes / Q000737:chemistry / Q000494:pharmacology*; D015536:Down-Regulation / Q000187:drug effects*; D007249:Inflammation; D020128:Inhibitory Concentration 50; D008070:Lipopolysaccharides; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008810:Mice, Inbred C57BL; D015394:Molecular Structure; D027343:Rhizome / Q000737:chemistry*; D030521:Zingiberaceae / Q000737:chemistry*
chemical_list: D016207:Cytokines; D004224:Diterpenes; D008070:Lipopolysaccharides; C433017:labdane