title: Pap1p-dependent upregulation of thioredoxin 3 and thioredoxin reductase genes from the fission yeast under nitrosative stress.
abstract: The thioredoxin system, consisting of thioredoxin, thioredoxin reductase, and NADPH, is involved in the response against a variety of stresses. The TRX3(+) and TrxR(+) genes encode thioredoxin 3 and thioredoxin reductase, respectively, in the fission yeast  Schizosaccharomyces pombe . Their transcriptional regulations were studied using the lacZ fusion genes. Synthesis of β-galactosidase from the TRX3(+)-lacZ fusion gene was markedly enhanced by nitric-oxide-generating sodium nitroprusside in the Pap1p-positive cells but not in the Pap1p-negative cells. Similarly, synthesis of β-galactosidase from the TrxR(+)-lacZ fusion gene was upregulated by sodium nitroprusside in a Pap1p-dependent manner. Synthesis of β-galactosidase from the TRX3(+)-lacZ and TrxR(+)-lacZ fusion genes was also enhanced by S-nitrosoglutathione in the Pap1p-positive cells but not in the Pap1p-negative cells. In brief, the S. pombe genes encoding thioredoxin 3 and thioredoxin reductase are upregulated under nitrosative stress in a Pap1p-dependent manner.
authors.affiliation: Department of Biochemistry, College of Natural Sciences, Kangwon National University, Chuncheon, Korea.
authors.forename: Min-Sik
authors.initials: MS
authors.lastname: Park
authors.forename: Hyeon-Jung
authors.initials: HJ
authors.lastname: Kim
authors.forename: A Rum
authors.initials: AR
authors.lastname: Park
authors.forename: Kisup
authors.initials: K
authors.lastname: Ahn
authors.forename: Hye-Won
authors.initials: HW
authors.lastname: Lim
authors.forename: Chang-Jin
authors.initials: CJ
authors.lastname: Lim
mesh_terms: D005786:Gene Expression Regulation / Q000187:drug effects; D015966:Gene Expression Regulation, Fungal; D009599:Nitroprusside / Q000378:metabolism; D026422:S-Nitrosoglutathione; D012568:Schizosaccharomyces / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological; D013880:Thioredoxin-Disulfide Reductase / Q000235:genetics* / Q000378:metabolism; D013879:Thioredoxins / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D029702:Schizosaccharomyces pombe Proteins; D009599:Nitroprusside; D013879:Thioredoxins; D026422:S-Nitrosoglutathione; D013880:Thioredoxin-Disulfide Reductase; D001616:beta-Galactosidase