title: Obtention of plant peroxidase and its potential for the decolorization of the reactive dye Remazol Turquoise G 133%.
abstract: Peroxidases can be used in the decolorization process. There is a growing interest for new sources of this enzyme and for obtaining economically viable processes. In this work, a low-cost vegetable peroxidase extraction process is proposed; the resulting enzyme is characterized to determine its optimum pH, temperature, and stability conditions, and it is then applied in the decolorization of reactive dye Remazol Turquoise G 133%. The turnip peroxidase (TP) was utilized as an enzymatic source. This enzyme exhibited maximum activity at pH 7.0, and it was active in the temperature range of 30 to 50 °C, which favors its use in industrial processes. Acetone was the most efficient solvent to induce precipitation. The removal of Remazol Turquoise G 133% was 56.0% complete after 50 min, while 41.0% of the same dye was removed with the commercial horseradish peroxidase enzyme in 50 min. TP presents potential as a viable alternative in the decolorization of textile wastewaters.
authors.affiliation: Department of Chemistry, Federal University of Lavras, 37200-000, Lavras, MG, Brazil. julianaarriel@hotmail.com
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Silva
authors.forename: Juliana Arriel
authors.initials: JA
authors.lastname: Torres
authors.forename: Angelita Duarte
authors.initials: AD
authors.lastname: Corrêa
authors.forename: Allana Maria Bernardes
authors.initials: AM
authors.lastname: Junqueira
authors.forename: Maria Teresa Pessoa
authors.initials: MT
authors.lastname: Amorim
authors.forename: Custódio Donizete
authors.initials: CD
authors.lastname: dos Santos
mesh_terms: D004396:Coloring Agents / Q000737:chemistry / Q000378:metabolism*; D056831:Coordination Complexes / Q000737:chemistry / Q000378:metabolism*; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D007211:Indoles / Q000737:chemistry / Q000378:metabolism*; D007220:Industrial Waste; D010544:Peroxidases / Q000737:chemistry / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D013783:Textile Industry; D014865:Waste Disposal, Fluid / Q000379:methods; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000378:metabolism*
chemical_list: D004396:Coloring Agents; D056831:Coordination Complexes; D007211:Indoles; D007220:Industrial Waste; D010940:Plant Proteins; D014874:Water Pollutants, Chemical; C558160:remazol turquoise blue G 133; D010544:Peroxidases