title: Development of eight mitochondrial markers for Funariaceae (Musci) and their amplification success in other mosses.
abstract: OBJECTIVE
  In comparison to the wide use of chloroplast markers, few mitochondrial markers are available for phylogenetic studies in bryophytes. We investigated the phylogenetic suitability of several mtDNA markers within the Funariaceae and across mosses.
  
  
  RESULTS
  By comparing mitochondrial genomes of two mosses, eight regions with higher substitution rates were identified and sequenced for three species in the Funariaceae and one outgroup taxon. Variations in the substitution rate of these new loci were compared to previously used markers. Thirty-four samples representing all major moss lineages were targeted to assess the universality of the newly designed primers.
  
  
  CONCLUSIONS
  The new markers provided similar or more sequence variations in Funariaceae compared to previously developed mtDNA markers. Five out of eight loci were amplified in 70% of other taxa, indicating that these markers may be suitable for phylogenetic studies in other moss lineages.
authors.affiliation: Department of Ecology and Evolutionary Biology, 75 North Eagleville Road, University of Connecticut, Storrs, Connecticut 06269-3043, USA. yang.liu@uconn.edu
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Nicholas L
authors.initials: NL
authors.lastname: Moskwa
authors.forename: Bernard
authors.initials: B
authors.lastname: Goffinet
mesh_terms: D001483:Base Sequence; D044002:Bryophyta / Q000145:classification / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D030541:Databases, Genetic; D056426:Genetic Loci; D005819:Genetic Markers; D054643:INDEL Mutation; D008928:Mitochondria / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D004272:DNA, Mitochondrial; D018744:DNA, Plant; D005819:Genetic Markers