title: Alteration of sensory-evoked metabolic and oscillatory activities in the olfactory bulb of GLAST-deficient mice.
abstract: Astrocytes are key cellular elements in both the tripartite synapse and the neurovascular unit. To fulfill this dual role in synaptic activity and metabolism, they express a panel of receptors and transporters that sense glutamate. Among them, the GLT-1 and GLAST transporters are known to regulate extracellular glutamate concentrations at excitatory synapses and consequently modulate glutamate receptor signaling. These major uptake systems are also involved in energy supply to neurons. However, the functional role of GLAST in concurrent regulation of metabolic and neuronal activity is currently unknown. We took advantage of the attractive structural and functional features of the main olfactory bulb to explore the impact of GLAST on sensory information processing while probing both glutamate uptake and neuronal activity in glomeruli and deeper cellular layers, respectively. Using odor-evoked 2-deoxyglucose imaging and local field potential recordings in GLAST knockout mice, we show in vivo that deletion of GLAST alters both glucose uptake and neuronal oscillations in olfactory bulb networks.
authors.affiliation: Laboratoire Imagerie et Modélisation en Neurobiologie et Cancérologie UMR 8165, Université Paris-Sud, CNRS, Orsay, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Martin
authors.forename: Diane
authors.initials: D
authors.lastname: Houitte
authors.forename: Martine
authors.initials: M
authors.lastname: Guillermier
authors.forename: Fanny
authors.initials: F
authors.lastname: Petit
authors.forename: Gilles
authors.initials: G
authors.lastname: Bonvento
authors.forename: Hirac
authors.initials: H
authors.lastname: Gurden