title: Characterization of functional, safety, and probiotic properties of Enterococcus faecalis UGRA10, a new AS-48-producer strain.
abstract: Enterococcus faecalis UGRA10, a new AS-48-producer strain, has been isolated from a Spanish sheep's cheese. The inhibitory substance produced by E. faecalis UGRA10 was purified and characterized using matrix-assisted laser desorption ionization-time of flight mass spectrometry, confirming its identity with AS-48 enterocin (7.150 Da). Subsequent genetic analysis showed the existence of the as-48 gene cluster on a plasmid of approximately 70-kb. The UGRA10 strain was examined for safety properties such as enterococci virulence genes, biogenic amine production, and antibiotic resistance. As for most E. faecalis strains, PCR amplification revealed the existence of gene encoding for GelE, Asa1, Esp, EfaA, and Ace antigens and for tyrosine decarboxylase. This strain was sensitive to most of the antibiotics tested, being resistant only to aminoglycosides, lincosamide, and pristinamicins. In addition, UGRA10 developed an ability to form biofilms and to adhere to Caco 2 and HeLa 229 cells. More interestingly, this strain shows a high ability to interfere with the adhesion of Listeria monocytogenes to Caco 2 cells. Altogether, the results suggest that this broad-spectrum bacteriocin-producing strain has biotechnological potential to be developed as a protective agent in food preservation and as a probiotic.
authors.affiliation: Departamento de Microbiología, Facultad de Ciencias, Universidad de Granada Fuente Nueva s/n, 19071-Granada, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Cebrián
authors.forename: A
authors.initials: A
authors.lastname: Baños
authors.forename: E
authors.initials: E
authors.lastname: Valdivia
authors.forename: R
authors.initials: R
authors.lastname: Pérez-Pulido
authors.forename: M
authors.initials: M
authors.lastname: Martínez-Bueno
authors.forename: M
authors.initials: M
authors.lastname: Maqueda
mesh_terms: D000617:Aminoglycosides / Q000378:metabolism; D000818:Animals; D001422:Bacterial Adhesion; D001430:Bacteriocins / Q000302:isolation & purification* / Q000494:pharmacology; D018938:Caco-2 Cells; D002611:Cheese / Q000382:microbiology* / Q000592:standards; D003257:Consumer Product Safety; D004269:DNA, Bacterial / Q000302:isolation & purification*; D024881:Drug Resistance, Bacterial / Q000187:drug effects; D013293:Enterococcus faecalis / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D005506:Food Contamination / Q000032:analysis; D005516:Food Microbiology / Q000379:methods; D006367:HeLa Cells; D006801:Humans; D055231:Lincosamides / Q000378:metabolism; D008089:Listeria monocytogenes / Q000472:pathogenicity; D008826:Microbial Sensitivity Tests; D005810:Multigene Family; D019936:Probiotics / Q000378:metabolism*; D012756:Sheep; D014774:Virulence
chemical_list: D000617:Aminoglycosides; D001430:Bacteriocins; D004269:DNA, Bacterial; D055231:Lincosamides