title: Association between DNA strand breakage and oxidative, inflammatory and endothelial biomarkers in type 2 diabetes.
abstract: Evidence has been presented recently that type 2 diabetes patients have an increased level of DNA damage. This DNA damage could be associated with oxidative, inflammatory, and endothelial biomarkers and could represent a possible indication of injury in the endothelium and induction of inflammation in type 2 diabetes. To confirm this possible association, DNA strand breakage was evaluated by use of the comet assay and its association with oxidative, inflammatory, and endothelial biomarkers in type 2 diabetes patients. A case-control study (30 healthy controls and 32 subjects with type 2 diabetes) was performed to evaluate the association between DNA damage and NOx (nitrate/nitrite), interleukin-6 (IL-6), urinary albumin, fasting glucose, and glycated hemoglobin (HbA(1c)) levels. Type 2 diabetes patients presented higher DNA damage than control subjects, higher levels of IL-6 and urinary albumin, and lower NOx. Significant correlations between DNA damage and NOx (r=-0.303, p=0.016), IL-6 (r=0.845, p<0.001), urinary albumin (r=0.496, p<0.001), fasting glucose (r=0.449, p<0.001), and HbA(1c) (r=0.575, p<0.001) were reported. Our findings showed an increase of DNA damage in type 2 diabetes especially in those patients with poor glycemic control and associations among NOx, IL-6 and urinary albumin levels with DNA damage.
authors.affiliation: Laboratório de Bioquímica Clínica, Departamento de Análises Clínicas e Toxicológicas, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil; Programa de Pós-Graduação em Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Etiane
authors.initials: E
authors.lastname: Tatsch
authors.forename: Guilherme V
authors.initials: GV
authors.lastname: Bochi
authors.forename: Sílvia J
authors.initials: SJ
authors.lastname: Piva
authors.forename: José A M
authors.initials: JA
authors.lastname: De Carvalho
authors.forename: Helena
authors.initials: H
authors.lastname: Kober
authors.forename: Vanessa D
authors.initials: VD
authors.lastname: Torbitz
authors.forename: Thiago
authors.initials: T
authors.lastname: Duarte
authors.forename: Cristiane
authors.initials: C
authors.lastname: Signor
authors.forename: Adriane C
authors.initials: AC
authors.lastname: Coelho
authors.forename: Marta M M F
authors.initials: MM
authors.lastname: Duarte
authors.forename: Greice F F S
authors.initials: GF
authors.lastname: Montagner
authors.forename: Ivana B M
authors.initials: IB
authors.lastname: Da Cruz
authors.forename: Rafael N
authors.initials: RN
authors.lastname: Moresco
mesh_terms: D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D053903:DNA Breaks, Double-Stranded; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000235:genetics / Q000473:pathology; D042783:Endothelial Cells / Q000378:metabolism; D004730:Endothelium, Vascular / Q000473:pathology; D005260:Female; D006801:Humans; D018836:Inflammation Mediators / Q000097:blood*; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D018384:Oxidative Stress
chemical_list: D015415:Biomarkers; D018836:Inflammation Mediators