title: [Study on differentiation of rat adipose tissue-derived stromal cells into Schwann-like cells].
abstract: OBJECTIVE
  To investigate the phenotypic, molecular and biological characteristics of adipose tissue-derived stromal cells (ADSCs) differentiated alonely a Schwann cells (SCs) lineage and to provide a new cells' seed source for nerve tissue engineering or cell therapy.
  
  
  METHODS
  Cultured ADSCs were isolated from SD rats and the undifferentiated ADSCs were confirmed by detection of MSC-specific cell-surface markers. The ADSCs were differentiated along a glial cell lineage using an established cocktail of growth factors. Following differention, we used immunofluorescene staining and RT-PCR to evaluate the characteristics of differentiated WJMSCs.
  
  
  RESULTS
  ADSCs were successfully isolated from the rats' fat tissue. The isolated ADSCs expressed CD29, CD90 but not CD34, CD44 nor CD45. Osteogenic differentiation was detected by Alizarin red staining and adipogenic differentiation was comfirmed by Oil-red O staining. ADSCs treated with a mixture of glial growth factors adopted a spindle-like morphology similar to Schwann cells. Immunocytochemical staining and RT-PCR analysis revealed that the treated cells expressed the glial markers S100, P75 and glial fibrillary acidic protein indicative of differentiation.
  
  
  CONCLUSIONS
  ADSCs can be differentiated into cells that are Schwann-like in terms of morphologic features and phenotype and could be suitable Schwann-cell substitutes for nerve repair in clinical applications.
authors.affiliation: Orthopaedic Research Institute of PLA General Hospital, Beijing 100853, China.
authors.forename: Zhi-Wu
authors.initials: ZW
authors.lastname: Ren
authors.forename: Zhe
authors.initials: Z
authors.lastname: Zhao
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Ji-Feng
authors.initials: JF
authors.lastname: Chen
authors.forename: Sheng-Feng
authors.initials: SF
authors.lastname: Zhan
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Wen-Jing
authors.initials: WJ
authors.lastname: Xu
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Jiang
authors.initials: J
authors.lastname: Peng
authors.forename: Shi-Bi
authors.initials: SB
authors.lastname: Lu
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000166:cytology*