title: Evaporative light scattering detection based HPLC method for the determination of polysorbate 80 in therapeutic protein formulations.
abstract: An evaporative light scattering detection (ELSD) based high-performance liquid chromatography (HPLC) method is developed for the determination of polysorbate 80 (tween 80) in therapeutic protein formulations. The method is simple and overcomes the difficulties associated with specificity and sensitivity. The method is suitable for the quantitation of polysorbate 80 in the usual formulation range (0.01-0.1%) as well as in trace amounts ≥13 µg/mL. The analysis is based on the removal of protein first by solid-phase extraction using Oasis HLB cartridges followed by HPLC analysis using Inertsil ODS-3 C 18 column (4.6×150 mm, 5 µm) using reversed-phase conditions. The detector response changes exponentially with an increase in polysorbate concentration. A very good linear fit of log ELSD response against log polysorbate 80 concentration is observed. The specificity, sensitivity, precision, and accuracy of the method are suitable for the quantitation of polysorbate 80 in protein formulations.
authors.affiliation: Analytical Development & Testing, Bristol-Myers Squibb Company, 6000 Thompson Road, Syracuse, NY 13057, USA.
authors.forename: Vikram S
authors.initials: VS
authors.lastname: Nayak
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Tan
authors.forename: Peter M
authors.initials: PM
authors.lastname: Ihnat
authors.forename: Reb J
authors.initials: RJ
authors.lastname: Russell
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Grace
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase; D004044:Dietary Proteins / Q000032:analysis*; D005526:Food, Formulated / Q000032:analysis*; D008027:Light; D016014:Linear Models; D011136:Polysorbates / Q000032:analysis*; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity
chemical_list: D004044:Dietary Proteins; D011136:Polysorbates