title: Use of amplified-fragment length polymorphism to study the ecology of Campylobacter jejuni in environmental water and to predict multilocus sequence typing clonal complexes.
abstract: We determined the genetic variability among water isolates of Campylobacter jejuni by using amplified-fragment length polymorphism (AFLP) and multilocus sequence typing (MLST). Across a highly diverse collection of isolates, AFLP clusters did not correlate with MLST clonal complexes, suggesting that AFLP is not reliable for deciphering population genetic relationships and may be problematic for larger epidemiologic analyses.
authors.affiliation: Department of Microbiology and Infectious Diseases, Faculté de Médecine de l’Université de Sherbrooke, Québec, Canada.
authors.forename: Simon
authors.initials: S
authors.lastname: Lévesque
authors.forename: Karen
authors.initials: K
authors.lastname: St-Pierre
authors.forename: Eric
authors.initials: E
authors.lastname: Frost
authors.forename: Robert D
authors.initials: RD
authors.lastname: Arbeit
authors.forename: Sophie
authors.initials: S
authors.lastname: Michaud
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis / Q000379:methods*; D015373:Bacterial Typing Techniques; D016123:Campylobacter jejuni / Q000145:classification* / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification; D017753:Ecosystem; D004784:Environmental Monitoring / Q000379:methods; D014644:Genetic Variation; D005828:Genetics, Population; D058885:Multilocus Sequence Typing / Q000379:methods*; D011237:Predictive Value of Tests; D011792:Quebec; D012621:Seasons; D014871:Water Microbiology