title: Microencapsulated Saccharomyces cerevisiae column bioreactor for potential use in renal failure uremia.
abstract: A novel bioreactor containing viable APA microencapsulated yeast cells was designed. Rat plasma was used for perfusion. Yeast cell loading and perfusion flow rate were studied to maximize urea removal. An increase in column loading from 25% to 100%, increased urea removal from 5.67 ± 1.34% to 30.45 ± 0.48%. An increase in flow rate from low to high, increased urea removal from 30.46% to 40.4%. At 100% column loading and high flow rate, the creatinine and phosphate concentrations decreased by 22% and 10%, respectively, while ammonia concentrations increased by 58.9% (p < 0.05). Our in-vitro perfusion study demonstrates that microencapsulated yeast cells can remove urea efficiently.
authors.affiliation: Department of Biomedical Engineering, Faculty of Medicine, McGill University, Montreal, Quebec, Canada.
authors.forename: Razek Georges
authors.initials: RG
authors.lastname: Coussa
authors.forename: Sapna
authors.initials: S
authors.lastname: Shah
authors.forename: Poonam
authors.initials: P
authors.lastname: Jain
authors.forename: Christopher
authors.initials: C
authors.lastname: Martoni
authors.forename: Jasmine
authors.initials: J
authors.lastname: Bhathena
authors.forename: Meenakshi
authors.initials: M
authors.lastname: Malhotra
authors.forename: Satya
authors.initials: S
authors.lastname: Prakash
mesh_terms: D000641:Ammonia / Q000097:blood; D000818:Animals; D019149:Bioreactors / Q000382:microbiology*; D002214:Capsules; D002470:Cell Survival; D003404:Creatinine / Q000097:blood; D010477:Perfusion; D010710:Phosphates / Q000097:blood; D010949:Plasma; D051381:Rats; D051437:Renal Insufficiency / Q000150:complications*; D017582:Renal Replacement Therapy / Q000295:instrumentation*; D012441:Saccharomyces cerevisiae / Q000166:cytology*; D014508:Urea / Q000097:blood; D014511:Uremia / Q000150:complications* / Q000628:therapy*
chemical_list: D002214:Capsules; D010710:Phosphates; D000641:Ammonia; D014508:Urea; D003404:Creatinine