title: Molecularly imprinted polymer as sorbent in micro-solid phase extraction of ochratoxin A in coffee, grape juice and urine.
abstract: A simple, environmental friendly and selective sample preparation technique employing porous membrane protected micro-solid phase extraction (μ-SPE) loaded with molecularly imprinted polymer (MIP) for the determination of ochratoxin A (OTA) is described. After the extraction, the analyte was desorbed using ultrasonication and was analyzed using high performance liquid chromatography. Under the optimized conditions, the detection limits of OTA for coffee, grape juice and urine were 0.06 ng g(-1), 0.02 and 0.02 ng mL(-1), respectively while the quantification limits were 0.19 ng g(-1), 0.06 and 0.08 ng mL(-1), respectively. The recoveries of OTA from coffee spiked at 1, 25 and 50 ng g(-1), grape juice and urine samples at 1, 25 and 50 ng mL(-1) ranged from 90.6 to 101.5%. The proposed method was applied to thirty-eight samples of coffee, grape juice and urine and the presence of OTA was found in eighteen samples. The levels found, however, were all below the legal limits.
authors.affiliation: School of Chemical Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia.
authors.forename: Tien Ping
authors.initials: TP
authors.lastname: Lee
authors.forename: Bahruddin
authors.initials: B
authors.lastname: Saad
authors.forename: Wejdan Shakir
authors.initials: WS
authors.lastname: Khayoon
authors.forename: Baharuddin
authors.initials: B
authors.lastname: Salleh
mesh_terms: D000327:Adsorption; D000328:Adult; D001628:Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D003069:Coffee / Q000737:chemistry; D005506:Food Contamination; D006801:Humans; D008875:Middle Aged; D054802:Molecular Imprinting; D009183:Mycotoxins / Q000652:urine*; D009793:Ochratoxins / Q000652:urine*; D016062:Porosity; D012680:Sensitivity and Specificity; D052617:Solid Phase Microextraction / Q000379:methods*; D013010:Sonication; D027843:Vitis / Q000737:chemistry*
chemical_list: D003069:Coffee; D009183:Mycotoxins; D009793:Ochratoxins; C025589:ochratoxin A