title: [GLI-1 is involved in EGF-regulated enhancement of the invasiveness of prostate cancer ARCaP(E) cells in vitro].
abstract: OBJECTIVE
  To investigate the role of the hedgehog (HH) signaling pathway transcription factor glioma-associated oncogene hoinolog 1 (GLI-1) in EGF-regulated enhancement of the invasiveness of the prostate cancer ARCaP(E) cell line in vitro.
  
  
  METHODS
  The expressions of EGFR and GLI-1 in prostate cancer ARCaP(E) cells were analyzed by immunofluorescence staining. ARCaP(E) cells were treated with EGF at 100 ng/ml, followed by detection of the changes in cell morphology and invasiveness, as well as in the expressions of p-ERK, ERK and GLI-1. Migration transwell assay was used to determine the effects of 100 ng/ml EGF and GLI-1 antagonist GANT61 on the invasiveness of the ARCaP(E) cells.
  
  
  RESULTS
  Both EGFR and GLI-1 were expressed in the ARCaP(E) cells. EGF induced morphological transition of epithelial-like ARCaP(E) cells to mesenchymal-like cells, increased their in vitro invasiveness, and significantly upregulated the expressions of p-ERK and GLI-1 in the ARCaP(E) cells (P<0.05). GANT61 significantly inhibited the in vitro invasiveness of the ARCaP(E) cells and reduced the enhancing effect of EGF on their invasiveness (P<0.05).
  
  
  CONCLUSIONS
  The results from ARCaP(E) cells shed light on the cross-talk of the HH pathway with the EGF/ERK signaling pathway. GLI-1 might be responsible for EGF-regulated enhancement of the invasiveness of ARCaP(E) cells in vitro.
authors.affiliation: Department of Urology, Ministry of Education, The First Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China. zhugdxjtu@yahoo.com.cn
authors.forename: Guo-dong
authors.initials: GD
authors.lastname: Zhu
authors.forename: Jian-cheng
authors.initials: JC
authors.lastname: Zhou
authors.forename: Jin
authors.initials: J
authors.lastname: Zeng
authors.forename: Zhen-kun
authors.initials: ZK
authors.lastname: Ma
authors.forename: Bo-xing
authors.initials: BX
authors.lastname: Su
authors.forename: Xin-yang
authors.initials: XY
authors.lastname: Wang
authors.forename: Da-lin
authors.initials: DL
authors.lastname: He
mesh_terms: D045744:Cell Line, Tumor; D004815:Epidermal Growth Factor / Q000378:metabolism*; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology*; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D000071676:Zinc Finger Protein GLI1
chemical_list: C400551:GLI1 protein, human; D014157:Transcription Factors; D000071676:Zinc Finger Protein GLI1; D004815:Epidermal Growth Factor