title: Insights into hemoglobin assembly through in vivo mutagenesis of α-hemoglobin stabilizing protein.
abstract: α-Hemoglobin stabilizing protein (AHSP) is believed to facilitate adult Hemoglobin A assembly and protect against toxic free α-globin subunits. Recombinant AHSP binds multiple forms of free α-globin to stabilize their structures and inhibit precipitation. However, AHSP also stimulates autooxidation of αO(2) subunit and its rapid conversion to a partially unfolded bishistidyl hemichrome structure. To investigate these biochemical properties, we altered the evolutionarily conserved AHSP proline 30 in recombinantly expressed proteins and introduced identical mutations into the endogenous murine Ahsp gene. In vitro, the P30W AHSP variant bound oxygenated α chains with 30-fold increased affinity. Both P30W and P30A mutant proteins also caused decreased rates of αO(2) autooxidation as compared with wild-type AHSP. Despite these abnormalities, mice harboring P30A or P30W Ahsp mutations exhibited no detectable defects in erythropoiesis at steady state or during induced stresses. Further biochemical studies revealed that the AHSP P30A and P30W substitutions had minimal effects on AHSP interactions with ferric α subunits. Together, our findings indicate that the ability of AHSP to stabilize nascent α chain folding intermediates prior to hemin reduction and incorporation into adult Hemoglobin A is physiologically more important than AHSP interactions with ferrous αO(2) subunits.
authors.affiliation: Cell and Molecular Biology Graduate Group, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
authors.forename: Eugene
authors.initials: E
authors.lastname: Khandros
authors.forename: Todd L
authors.initials: TL
authors.lastname: Mollan
authors.forename: Xiang
authors.initials: X
authors.lastname: Yu
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Wang
authors.forename: Yu
authors.initials: Y
authors.lastname: Yao
authors.forename: Janine
authors.initials: J
authors.lastname: D'Souza
authors.forename: David A
authors.initials: DA
authors.lastname: Gell
authors.forename: John S
authors.initials: JS
authors.lastname: Olson
authors.forename: Mitchell J
authors.initials: MJ
authors.lastname: Weiss
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D004920:Erythropoiesis; D055879:Gene Knock-In Techniques; D006427:Hemin / Q000378:metabolism; D006441:Hemoglobin A / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008706:Methemoglobin / Q000737:chemistry / Q000378:metabolism; D051379:Mice; D008958:Models, Molecular; D018832:Molecular Chaperones / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D016296:Mutagenesis; D050505:Mutant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D010084:Oxidation-Reduction; D011487:Protein Conformation; D055503:Protein Multimerization / Q000235:genetics; D017086:beta-Thalassemia / Q000235:genetics
chemical_list: C511177:Ahsp protein, mouse; D018832:Molecular Chaperones; D050505:Mutant Proteins; D006427:Hemin; D008706:Methemoglobin; D006441:Hemoglobin A