title: Purification of norovirus-like particles (VLPs) by ion exchange chromatography.
abstract: Recombinant expression of the norovirus capsid protein VP1 leads to self-assembly of non-infectious virus-like particles (VLPs), which are recognized as promising vaccine candidates against norovirus infections. To overcome the scalability issues connected to the ultracentrifugation-based purification strategies used in previous studies, an anion exchange-based purification method for norovirus VLPs was developed in this study. The method consists of precipitation by polyethylene glycol (PEG) and a single anion exchange chromatography step for purifying baculovirus-expressed GII.4 norovirus VLPs, which can be performed within one day. High product purity was obtained using chromatography. The purified material also contained fully assembled monodispersed VLPs, which were recognized by human sera containing polyclonal antibodies against norovirus GII.4.
authors.affiliation: Institute of Biomedical Technology, Biokatu 6, FI-33014 University of Tampere and Tampere University Hospital, Finland.
authors.forename: Tiia
authors.initials: T
authors.lastname: Koho
authors.forename: Tuomas
authors.initials: T
authors.lastname: Mäntylä
authors.forename: Pasi
authors.initials: P
authors.lastname: Laurinmäki
authors.forename: Leena
authors.initials: L
authors.lastname: Huhti
authors.forename: Sarah J
authors.initials: SJ
authors.lastname: Butcher
authors.forename: Timo
authors.initials: T
authors.lastname: Vesikari
authors.forename: Markku S
authors.initials: MS
authors.lastname: Kulomaa
authors.forename: Vesa P
authors.initials: VP
authors.lastname: Hytönen
mesh_terms: D016367:Baculoviridae; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism; D002852:Chromatography, Ion Exchange / Q000379:methods*; D005590:Fractional Precipitation; D005822:Genetic Vectors; D029322:Norovirus / Q000235:genetics*; D011092:Polyethylene Glycols / Q000737:chemistry; D014773:Virology / Q000379:methods*; D022701:Virosomes / Q000235:genetics* / Q000302:isolation & purification*
chemical_list: D036022:Capsid Proteins; D022701:Virosomes; D011092:Polyethylene Glycols