title: ¹H NMR-based serum metabolic profiling in compensated and decompensated cirrhosis.
abstract: OBJECTIVE
  To study the metabolic profiling of serum samples from compensated and decompensated cirrhosis patients.
  
  
  METHODS
  A pilot metabolic profiling study was conducted using three groups: compensated cirrhosis patients (n = 30), decompensated cirrhosis patients (n = 30) and healthy controls (n = 30). A ¹H nuclear magnetic resonance (NMR)-based metabonomics approach was used to obtain the serum metabolic profiles of the samples. The acquired data were processed by multivariate principal component analysis and orthogonal partial least-squares discriminant analysis (OPLS-DA).
  
  
  RESULTS
  The OPLS-DA model was capable of distinguishing between decompensated and compensated cirrhosis patients, with an R²Y of 0.784 and a Q²Y of 0.598. Twelve metabolites, such as pyruvate, phenylalanine and succinate, were identified as the most influential factors for the difference between the two groups. The validation of the diagnosis prediction showed that the accuracy of the OPLS-DA model was 85% (17/20).
  
  
  CONCLUSIONS
  ¹H NMR spectra combined with pattern recognition analysis techniques offer a new way to diagnose compensated and decompensated cirrhosis in the future.
authors.affiliation: Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Chongqing Medical University, Chongqing 410006, China.
authors.forename: Su-Wen
authors.initials: SW
authors.lastname: Qi
authors.forename: Zhi-Guang
authors.initials: ZG
authors.lastname: Tu
authors.forename: Wu-Jian
authors.initials: WJ
authors.lastname: Peng
authors.forename: Lin-Xian
authors.initials: LX
authors.lastname: Wang
authors.forename: Xin
authors.initials: X
authors.lastname: Ou-Yang
authors.forename: An-Ji
authors.initials: AJ
authors.lastname: Cai
authors.forename: Yong
authors.initials: Y
authors.lastname: Dai
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D008103:Liver Cirrhosis / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology*; D008297:Male; D055442:Metabolome; D008875:Middle Aged; D015233:Models, Statistical; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D010865:Pilot Projects; D025341:Principal Component Analysis; D015203:Reproducibility of Results