title: miR-200b is involved in intestinal fibrosis of Crohn's disease.
abstract: Intestinal fibrosis is one of the major serious complications of Crohn's disease (CD). However, there are no effective antifibrotic drugs to treat intestinal fibrosis in CD. Therefore, it is important to understand the pathogenesis of fibrosis in CD. It has been reported that members of the miR-200 family are essential in the regulation of renal fibrogenesis. In this study, we analyzed the function of miR-200a and miR-200b in intestinal fibrosis, which was induced by transforming growth factor β1 (TGF-β1) in vitro. Furthermore, we detected the expression of miR-200a and miR-200b in CD specimens, which were divided into groups of fibrosis and no-fibrosis. The results of this study showed that administration of miR-200b could partially protect intestinal epithelial cells from fibrogenesis in vitro. Furthermore, we found that miR-200b was overexpressed in the serum of the fibrosis group. The results suggest that miR-200b has potential value for diagnostic and therapeutic applications for CD patients with fibrosis complications.
authors.affiliation: Department of Gastroenterology, Shanghai Jiao Tong University, School of Medicine, Xin Hua Hospital, Shanghai, PR China.
authors.forename: Yingwei
authors.initials: Y
authors.lastname: Chen
authors.forename: Wensong
authors.initials: W
authors.lastname: Ge
authors.forename: Leiming
authors.initials: L
authors.lastname: Xu
authors.forename: Chunying
authors.initials: C
authors.lastname: Qu
authors.forename: Mingjie
authors.initials: M
authors.lastname: Zhu
authors.forename: Wenzhu
authors.initials: W
authors.lastname: Zhang
authors.forename: Yongtao
authors.initials: Y
authors.lastname: Xiao
mesh_terms: D002478:Cells, Cultured; D003424:Crohn Disease / Q000235:genetics* / Q000473:pathology*; D015536:Down-Regulation; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D005355:Fibrosis; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000473:pathology*; D035683:MicroRNAs / Q000008:administration & dosage / Q000235:genetics* / Q000378:metabolism*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
chemical_list: C533896:MIRN200 microRNA, human; D035683:MicroRNAs; D053773:Transforming Growth Factor beta1