title: A new stable and reliable method for labeling nucleic acids of fully replicative viruses.
abstract: Efficiently labeling nucleic acids of fully replicative viruses is a challenge. In this work, a 'molecular light switch' complex [Ru(phen)(2)(dppz)](2+), where phen = 1,10-phenanthroline and dppz = dipyrido[3,2-a:2',3'-c]phenazine, has been exploringly used to label vaccinia virus nucleic acid. The labeled virions exhibited strong and stable fluorescence and could be imaged at the single-virion level. Moreover, they were fully infectious and can be used to study the behaviors of invasion into their host cells. The method is general and suitable for labeling various DNA viruses.
authors.affiliation: School of Life Science, Beijing Institute of Technology, Beijing 100081, China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Huang
authors.forename: Peng
authors.initials: P
authors.lastname: Zhou
authors.forename: Han-Zhong
authors.initials: HZ
authors.lastname: Wang
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.forename: Jian
authors.initials: J
authors.lastname: Hao
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Xie
authors.forename: Zhi-Ke
authors.initials: ZK
authors.lastname: He
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D004279:DNA, Viral / Q000378:metabolism*; D009942:Organometallic Compounds / Q000737:chemistry / Q000378:metabolism; D010618:Phenanthrolines / Q000737:chemistry; D015203:Reproducibility of Results; D012428:Ruthenium / Q000737:chemistry; D013194:Staining and Labeling / Q000379:methods*; D014616:Vaccinia virus / Q000502:physiology*; D014709:Vero Cells; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D009942:Organometallic Compounds; D010618:Phenanthrolines; D012428:Ruthenium