title: HIV-1 DNA is detected in bone marrow populations containing CD4+ T cells but is not found in purified CD34+ hematopoietic progenitor cells in most patients on antiretroviral therapy.
abstract: Identifying cellular reservoirs of human immunodeficiency virus type 1 (HIV-1) in patients on antiretroviral therapy (ART) is critical to finding a cure for HIV-1. In addition to resting CD4(+) T cells, CD34(+) hematopoietic progenitor cells have been proposed as another reservoir. We obtained bone marrow aspirates from 11 patients on ART who had undetectable plasma HIV-1 RNA. HIV-1 DNA was detected in CD4(+) T cells from peripheral blood in all patients and from bone marrow cellular fractions containing T cells in most patients. We did not find HIV-1 DNA in highly purified CD34(+) populations using either a sensitive real-time polymerase chain reaction assay or a coculture assay for replication-competent HIV-1.
authors.affiliation: Department of Medicine, The Sidney Kimmel CancerCenter, USA. cdurand2@jhmi.edu
authors.forename: Christine M
authors.initials: CM
authors.lastname: Durand
authors.forename: Gabriel
authors.initials: G
authors.lastname: Ghiaur
authors.forename: Janet D
authors.initials: JD
authors.lastname: Siliciano
authors.forename: S Alireza
authors.initials: SA
authors.lastname: Rabi
authors.forename: Evelyn E
authors.initials: EE
authors.lastname: Eisele
authors.forename: Maria
authors.initials: M
authors.lastname: Salgado
authors.forename: Liang
authors.initials: L
authors.lastname: Shan
authors.forename: Jun F
authors.initials: JF
authors.lastname: Lai
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.forename: Joseph
authors.initials: J
authors.lastname: Margolick
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Jones
authors.forename: Joel E
authors.initials: JE
authors.lastname: Gallant
authors.forename: Richard F
authors.initials: RF
authors.lastname: Ambinder
authors.forename: Robert F
authors.initials: RF
authors.lastname: Siliciano
mesh_terms: D000328:Adult; D000368:Aged; D044966:Anti-Retroviral Agents / Q000627:therapeutic use; D018952:Antigens, CD34 / Q000032:analysis; D001853:Bone Marrow / Q000737:chemistry / Q000821:virology; D001854:Bone Marrow Cells / Q000737:chemistry / Q000821:virology*; D015496:CD4-Positive T-Lymphocytes / Q000737:chemistry / Q000821:virology*; D004279:DNA, Viral / Q000302:isolation & purification*; D015658:HIV Infections / Q000276:immunology; D015497:HIV-1 / Q000302:isolation & purification / Q000472:pathogenicity / Q000502:physiology; D006412:Hematopoietic Stem Cells / Q000737:chemistry / Q000821:virology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction; D014779:Virus Replication
chemical_list: D044966:Anti-Retroviral Agents; D018952:Antigens, CD34; D004279:DNA, Viral