title: Endocytosis by Numb breaks Notch symmetry at cytokinesis.
abstract: Cell-fate diversity can be generated by the unequal segregation of the Notch regulator Numb at mitosis in both vertebrates and invertebrates. Whereas the mechanisms underlying unequal inheritance of Numb are understood, how Numb antagonizes Notch has remained unsolved. Live imaging of Notch in sensory organ precursor cells revealed that nuclear Notch is detected at cytokinesis in the daughter cell that does not inherit Numb. Numb and Sanpodo act together to regulate Notch trafficking and establish directional Notch signalling at cytokinesis. We propose that unequal segregation of Numb results in increased endocytosis in one daughter cell, hence asymmetry of Notch at the cytokinetic furrow, directional signalling and binary fate choice.
authors.affiliation: Institut Pasteur, Developmental Biology Department, F-75015 Paris, France.
authors.forename: Lydie
authors.initials: L
authors.lastname: Couturier
authors.forename: Nicolas
authors.initials: N
authors.lastname: Vodovar
authors.forename: François
authors.initials: F
authors.lastname: Schweisguth
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002467:Cell Nucleus / Q000378:metabolism; D048749:Cytokinesis; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D034281:Dynamins / Q000235:genetics / Q000378:metabolism; D004705:Endocytosis; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007605:Juvenile Hormones / Q000235:genetics / Q000378:metabolism*; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence; D009154:Mutation; D011485:Protein Binding; D034622:RNA Interference; D051880:Receptors, Notch / Q000235:genetics / Q000378:metabolism*; D059008:Time-Lapse Imaging
chemical_list: D029721:Drosophila Proteins; D007605:Juvenile Hormones; D008164:Luminescent Proteins; D008840:Microfilament Proteins; C497759:N protein, Drosophila; D051880:Receptors, Notch; C060606:numb protein, Drosophila; C112833:spdo protein, Drosophila; D049452:Green Fluorescent Proteins; D034281:Dynamins; C439628:shi protein, Drosophila