title: Effect of physicochemical characteristics of cellulosic substrates on enzymatic hydrolysis by means of a multi-stage process for cellobiose production.
abstract: The effect of two types of cellulose, microcrystalline cellulose and paper pulp, on enzymatic hydrolysis for cellobiose production was investigated. The particle size, the relative crystallinity index and the water retention value were determined for both celluloses. A previously studied multistage hydrolysis process that proved to enhance the cellobiose production was studied with both types of celluloses. The cellobiose yield exhibited a significant improvement (120% for the microcrystalline cellulose and 75% for the paper pulp) with the multistage hydrolysis process compared to continuous hydrolysis. The conversion of cellulose to cellobiose was greater for the microcrystalline cellulose than for the paper pulp. Even with high crystallinity, microcrystalline cellulose achieved the highest cellobiose yield probably due to its highest specific surface area accessible to enzymes and quantity of adsorbed protein.
authors.affiliation: Department of Industrial Biological Chemistry, Gembloux Agro-Bio Tech, University of Liège, Passage des Déportés 2, B-5030, Gembloux, Belgium. cvanderghem@ulg.ac.be
authors.forename: Caroline
authors.initials: C
authors.lastname: Vanderghem
authors.forename: Nicolas
authors.initials: N
authors.lastname: Jacquet
authors.forename: Sabine
authors.initials: S
authors.lastname: Danthine
authors.forename: Christophe
authors.initials: C
authors.lastname: Blecker
authors.forename: Michel
authors.initials: M
authors.lastname: Paquot
mesh_terms: D002475:Cellobiose / Q000737:chemistry*; D002480:Cellulase / Q000737:chemistry; D002482:Cellulose / Q000737:chemistry*; D055598:Chemical Phenomena; D004789:Enzyme Activation; D005947:Glucose / Q000737:chemistry; D006868:Hydrolysis; D010209:Paper; D010316:Particle Size; D011485:Protein Binding; D013379:Substrate Specificity; D013499:Surface Properties
chemical_list: D002475:Cellobiose; D002482:Cellulose; D002480:Cellulase; D005947:Glucose; C109691:microcrystalline cellulose