title: Dynamic imaging of homo-FRET in live cells by fluorescence anisotropy microscopy.
abstract: Multiple lipid and protein components of the plasma membrane of a living cell are organized, both compositionally and functionally, at different spatial and temporal scales. For instance, Rab protein domains in membranes the clathrin-coated pit, or the immunological synapse are exquisite examples of functional compartmentalization in cell membranes. These assemblies consist in part of nanoscale complexes of lipids and proteins and are necessary to facilitate some specific sorting and signaling functions. It is evident that cellular functions require a regulated spatiotemporal organization of components at the nanoscale, often comprising of countable number of molecular species. Here, we describe multiple homo-FRET-based imaging methods that provide information about nanoscale interactions between fluorescently tagged molecules in live cells, at optically resolved spatial resolution.
authors.affiliation: National Centre for Biological Science, Bangalore, India.
authors.forename: Subhasri
authors.initials: S
authors.lastname: Ghosh
authors.forename: Suvrajit
authors.initials: S
authors.lastname: Saha
authors.forename: Debanjan
authors.initials: D
authors.lastname: Goswami
authors.forename: Sameera
authors.initials: S
authors.lastname: Bilgrami
authors.forename: Satyajit
authors.initials: S
authors.lastname: Mayor
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D058948:Cell Tracking / Q000379:methods*; D004330:Drosophila / Q000166:cytology; D005454:Fluorescence Polarization / Q000295:instrumentation / Q000379:methods; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D049452:Green Fluorescent Proteins; D007091:Image Processing, Computer-Assisted; D050356:Lipid Metabolism; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*
chemical_list: D049452:Green Fluorescent Proteins