title: Backbone and side-chain 1H, 15N and 13C assignment of apo- and imipenem-acylated L,D-transpeptidase from Bacillus subtilis.
abstract: The D,D-transpeptidase activity of Penicillin Binding Proteins (PBPs) is essential to maintain cell wall integrity. PBPs catalyze the final step of the peptidoglycan synthesis by forming 4 → 3 cross-links between two peptide stems. Recently, a novel β-lactam resistance mechanism involving L,D-transpeptidases has been identified in Enterococcus faecium and Mycobacterium tuberculosis. In this resistance pathway, the classical 4 → 3 cross-links are replaced by 3 → 3 cross-links, whose formation are catalyzed by the L,D-transpeptidases. To date, only one class of the entire β-lactam family, the carbapenems, is able to inhibit the L,D-transpeptidase activity. Nevertheless, the specificity of this inactivation is still not understood. Hence, the study of this new transpeptidase family is of considerable interest in order to understand the mechanism of the L,D-transpeptidases inhibition by carbapenems. In this context, we present herein the backbone and side-chain (1)H, (15)N and (13)C NMR assignment of the L,D-transpeptidase from Bacillus subtilis (Ldt(Bs)) in the apo and in the acylated form with a carbapenem, the imipenem.
authors.affiliation: CEA, Institut de Biologie Structurale Jean-Pierre Ebel, 41, rue Jules Horowitz, 38027 Grenoble, France.
authors.forename: L
authors.initials: L
authors.lastname: Lecoq
authors.forename: C
authors.initials: C
authors.lastname: Bougault
authors.forename: T
authors.initials: T
authors.lastname: Kern
authors.forename: J-E
authors.initials: JE
authors.lastname: Hugonnet
authors.forename: C
authors.initials: C
authors.lastname: Veckerlé
authors.forename: O
authors.initials: O
authors.lastname: Pessey
authors.forename: M
authors.initials: M
authors.lastname: Arthur
authors.forename: J-P
authors.initials: JP
authors.lastname: Simorre
mesh_terms: D000215:Acylation; D000595:Amino Acid Sequence; D001059:Apoproteins / Q000737:chemistry*; D001412:Bacillus subtilis / Q000201:enzymology*; D002247:Carbon Isotopes; D015378:Imipenem / Q000378:metabolism*; D008969:Molecular Sequence Data; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular; D010458:Peptidyl Transferases / Q000737:chemistry*; D017433:Protein Structure, Secondary; D011522:Protons
chemical_list: D001059:Apoproteins; D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D011522:Protons; D015378:Imipenem; D010458:Peptidyl Transferases