title: Microarray analysis and phenotypic response of  Pseudomonas aeruginosa  PAO1 under hyperbaric oxyhelium conditions.
abstract: Pseudomonas aeruginosa  is an important opportunistic pathogen associated with multiple diseases including cystic fibrosis and nosocomial infections. Pseudomonas aeruginosa is also the microbe most often isolated from ear and skin infections in divers. Saturation divers often suffer from various skin and mucous disorders, of which P. aeruginosa infections are the most serious and frequent. Previous studies mainly focused on adaptive and regulatory mechanisms of P. aeruginosa virulence in inducing clinical acute and chronic infections under different environmental conditions. However, there are few studies describing the physiological adaptive and regulatory mechanisms of P. aeruginosa in inducing high infectivity in healthy divers under hyperbaric oxyhelium conditions and even fewer studies describing the overall influence of the hyperbaric oxyhelium environment on regulating mRNA and protein expression levels of P. aeruginosa. The present study used transcriptomic and virulence phenotype analysis to identify factors that allow P. aeruginosa to become established in a hyperbaric oxyhelium environment to facilitate infections in divers. Transcriptional profiling of P. aeruginosa grown under steady-state hyperbaric oxyhelium stress conditions showed an upregulation of genes associated with stress-sense/response, protein folding, transcriptional regulation, pili and flagellum metabolism, virulence adaptation, and membrane protein metabolism. Some of these genes (including several two-component systems not previously known to be influenced by hyperbaric oxyhelium) were differentially expressed by P. aeruginosa in response to 72 h of exposure to hyperbaric oxyhelium stress. Detection of the virulence phenotype confirmed the results of cDNA microarrays. Based on these results, we conclude that hyperbaric oxyhelium conditions affect PAO1 gene expression and upregulate the expression of most virulence genes. The data obtained in our study may provide new insight into the molecular mechanism of hyperbaric oxyhelium exposure against P. aeruginosa virulence adaptation.
authors.affiliation: Department of Ship Sanitation, Army Key Laboratory of Hyperbaric and Diving Physiology, The Institution of Naval Medicine, 880 Xiangyin Road, Shanghai, People's Republic of China. chen127shh@sina.com
authors.forename: Shuang-Hong
authors.initials: SH
authors.lastname: Chen
authors.forename: Rui-Yong
authors.initials: RY
authors.lastname: Chen
authors.forename: Xiong-Li
authors.initials: XL
authors.lastname: Xu
authors.forename: Wei-Bin
authors.initials: WB
authors.lastname: Xiao
mesh_terms: D000222:Adaptation, Physiological; D001274:Atmospheric Pressure; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D005407:Flagella / Q000378:metabolism; D046228:Microarray Analysis; D020411:Oligonucleotide Array Sequence Analysis; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D014774:Virulence / Q000235:genetics; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D037521:Virulence Factors