title: The influence of fatty acids on theophylline binding to human serum albumin. Comparative fluorescence study.
abstract: Theophylline, popular diuretic, is used to treat asthma and bronchospasm. In blood it forms complexes with albumin, which is also the main transporter of fatty acids. The aim of the present study was to describe the influence of fatty acids (FA) on binding of theophylline (Th) to human serum albumin (HSA) in the high affinity binding sites. Binding parameters have been obtained on the basis of the fluorescence analysis. The data obtained for the complex of Th and natural human serum albumin (nHSA) obtained from blood of obese patients qualified for surgical removal of stomach was compared with our previous studies on the influence of FA on the complex of Th and commercially available defatted human serum albumin (dHSA).
authors.affiliation: Department of Physical Pharmacy, Medical University of Silesia, Jagiellońska 4, 41-200 Sosnowiec, Poland. mmaciazek@sum.edu.pl
authors.forename: M
authors.initials: M
authors.lastname: Maciążek-Jurczyk
authors.forename: A
authors.initials: A
authors.lastname: Sułkowska
authors.forename: B
authors.initials: B
authors.lastname: Bojko
authors.forename: J
authors.initials: J
authors.lastname: Równicka-Zubik
authors.forename: A
authors.initials: A
authors.lastname: Szkudlarek-Haśnik
authors.forename: I
authors.initials: I
authors.lastname: Zubik-Skupień
authors.forename: A
authors.initials: A
authors.lastname: Góra
authors.forename: M
authors.initials: M
authors.lastname: Dubas
authors.forename: I
authors.initials: I
authors.lastname: Korzonek-Szlacheta
authors.forename: T
authors.initials: T
authors.lastname: Wielkoszyński
authors.forename: W
authors.initials: W
authors.lastname: Zurawiński
authors.forename: K
authors.initials: K
authors.lastname: Sosada
mesh_terms: D001665:Binding Sites; D001993:Bronchodilator Agents / Q000378:metabolism*; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008958:Models, Molecular; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013806:Theophylline / Q000378:metabolism*
chemical_list: D001993:Bronchodilator Agents; D005227:Fatty Acids; D012709:Serum Albumin; D013806:Theophylline