title: An impedimetric vascular endothelial growth factor biosensor-based PAMAM/cysteamine-modified gold electrode for monitoring of tumor growth.
abstract: In the present work, we have developed a new biosensor based on fourth-generation (G4) PAMAM dendrimers for the analysis of vascular endothelial growth factor (VEGF). First, the PAMAM dendrimers were covalently attached to a cysteamine-modified Au electrode by glutaraldehyde. With the help of the amino groups located on its surface, vascular endothelial growth factor receptor-1 (VEGF-R1) was immobilized via glutaraldehyde cross-linking. VEGF-R1 loading was investigated to identify the optimal VEGF-R1 immobilization conditions for the best sensitivity of the new biosensor. In addition, Kramers-Kronig transforms were also analyzed for immobilization and measurement processes. The biosensor had a linear range of 5 to 125 pg/mL VEGF. The fabricated biosensor had good repeatability and reproducibility. Finally, the results for artificial serum samples measured by the present biosensor showed a good recovery for VEGF detection.
authors.affiliation: Namık Kemal University, Faculty of Science, Chemistry Department, Biochemistry Division, Tekirdağ, Turkey.
authors.forename: Mustafa Kemal
authors.initials: MK
authors.lastname: Sezgintürk
authors.forename: Zihni Onur
authors.initials: ZO
authors.lastname: Uygun
mesh_terms: D015374:Biosensing Techniques; D003543:Cysteamine / Q000737:chemistry*; D050091:Dendrimers / Q000737:chemistry*; D058266:Dielectric Spectroscopy; D004566:Electrodes; D005976:Glutaral / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism; D009369:Neoplasms / Q000473:pathology*; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis*; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000737:chemistry / Q000378:metabolism
chemical_list: D050091:Dendrimers; D055767:Immobilized Proteins; C104700:PAMAM Starburst; D042461:Vascular Endothelial Growth Factor A; D003543:Cysteamine; D006046:Gold; D040281:Vascular Endothelial Growth Factor Receptor-1; D005976:Glutaral