title: Reversible immortalization of Nestin-positive precursor cells from pancreas and differentiation into insulin-secreting cells.
abstract: Pancreatic stem cells or progenitor cells posses the ability of directed differentiation into pancreatic β cells. However, these cells usually have limited proliferative capacity and finite lifespan in vitro. In the present study, Nestin-positive progenitor cells (NPPCs) from mouse pancreas that expressed the pancreatic stem cells or progenitor cell marker Nestin were isolated to obtain a sufficient number of differentiated pancreatic β cells. Tet-on system for SV40 large T-antigen expression in NPPCs was used to achieve reversible immortalization. The reversible immortal Nestin-positive progenitor cells (RINPPCs) can undergo at least 80 population doublings without senescence in vitro while maintaining their biological and genetic characteristics. RINPPCs can be efficiently induced to differentiate into insulin-producing cells that contain a combination of glucagon-like peptide-1 (GLP-1) and sodium butyrate. The results of the present study can be used to explore transplantation therapy of type I diabetes mellitus.
authors.affiliation: The Clinical Medical Research Center, The Second Clinical Medical College (Shenzhen People's Hospital), Jinan University, 518020 Shenzhen, PR China.
authors.forename: Pei
authors.initials: P
authors.lastname: Wei
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Qi
authors.forename: Han-Xin
authors.initials: HX
authors.lastname: Zhou
authors.forename: Chun-Yan
authors.initials: CY
authors.lastname: Deng
authors.forename: Fu-Rong
authors.initials: FR
authors.lastname: Li
mesh_terms: D053687:Adult Stem Cells / Q000737:chemistry / Q000166:cytology* / Q000187:drug effects; D000818:Animals; D002087:Butyrates / Q000494:pharmacology; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D002469:Cell Separation; D002478:Cells, Cultured; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D052216:Glucagon-Like Peptide 1 / Q000494:pharmacology; D050417:Insulin-Secreting Cells / Q000166:cytology*; D007381:Intermediate Filament Proteins / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D009419:Nerve Tissue Proteins / Q000032:analysis; D064231:Nestin
chemical_list: D002087:Butyrates; D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; D064231:Nestin; D052216:Glucagon-Like Peptide 1