title: Disruption of ptsG gene and manXYZ operon of ethanol-producing Escherichia coli KO11: Effects on glucose and xylose utilization and ethanol production.
abstract: Ethanol-producing Escherichia coli strain KO11 consumed 99% of the glucose and only 13% of the xylose in a mixture of glucose (60g/L) and xylose (40g/L) during the 72-h fermentation at 30°C. The deletion mutants ΔptsG, ΔmanXYZ, and ΔptsG/manXYZ utilized 42%, 78%, and 35% of the glucose and 50%, 32%, and 32% of the xylose, respectively.
authors.affiliation: Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuen Kibanadai Nishi, Miyazaki 889-2192, Japan. k.ohta@cc.miyazaki-u.ac.jp
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Ohta
authors.forename: Hironori
authors.initials: H
authors.lastname: Hamasuna
authors.forename: Junji
authors.initials: J
authors.lastname: Tsukamoto
authors.forename: Motoki
authors.initials: M
authors.lastname: Wakiyama
authors.forename: Yoshiya
authors.initials: Y
authors.lastname: Izumi
authors.forename: Keiko
authors.initials: K
authors.lastname: Harada
mesh_terms: D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005798:Genes, Bacterial / Q000235:genetics*; D005947:Glucose / Q000378:metabolism*; D009876:Operon / Q000235:genetics*; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000235:genetics; D017384:Sequence Deletion; D013997:Time Factors; D014994:Xylose / Q000378:metabolism*
chemical_list: D000431:Ethanol; D014994:Xylose; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C031629:phosphoenolpyruvate-glucose phosphotransferase; D005947:Glucose