title: Comparative analysis of transcriptomic and hormonal responses to compatible and incompatible plant-virus interactions that lead to cell death.
abstract: Hypersensitive response-related programmed cell death (PCD) has been extensively analyzed in various plant-virus interactions. However, little is known about the changes in gene expression and phytohormone levels associated with cell death caused by compatible viruses. The synergistic interaction of Potato virus X (PVX) with a number of Potyvirus spp. results in increased symptoms that lead to systemic necrosis (SN) in Nicotiana benthamiana. Here, we show that SN induced by a PVX recombinant virus expressing a potyviral helper component-proteinase (HC-Pro) gene is associated with PCD. We have also compared transcriptomic and hormonal changes that occur in response to a compatible synergistic virus interaction that leads to SN, a systemic incompatible interaction conferred by the Tobacco mosaic virus-resistance gene N, and a PCD response conditioned by depletion of proteasome function. Our analysis indicates that the SN response clusters with the incompatible response by the similarity of their overall gene expression profiles. However, the expression profiles of both defense-related genes and hormone-responsive genes, and also the relative accumulation of several hormones in response to SN, relate more closely to the response to depletion of proteasome function than to that elicited by the incompatible interaction. This suggests a potential contribution of proteasome dysfunction to the increased pathogenicity observed in PVX-Potyvirus mixed infections. Furthermore, silencing of coronatine insensitive 1, a gene involved in jasmonate perception, in N. benthamiana accelerated cell death induced by PVX expressing HC-Pro.
authors.affiliation: Departamento de Biología Medioambiental, Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040, Madrid, Spain.
authors.forename: Remedios
authors.initials: R
authors.lastname: Pacheco
authors.forename: Alberto
authors.initials: A
authors.lastname: García-Marcos
authors.forename: Aranzazu
authors.initials: A
authors.lastname: Manzano
authors.forename: Mario García
authors.initials: MG
authors.lastname: de Lacoba
authors.forename: Gemma
authors.initials: G
authors.lastname: Camañes
authors.forename: Pilar
authors.initials: P
authors.lastname: García-Agustín
authors.forename: José Ramón
authors.initials: JR
authors.lastname: Díaz-Ruíz
authors.forename: Francisco
authors.initials: F
authors.lastname: Tenllado
mesh_terms: D016923:Cell Death; D016000:Cluster Analysis; D003546:Cysteine Endopeptidases / Q000235:genetics*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D054884:Host-Pathogen Interactions; D020411:Oligonucleotide Array Sequence Analysis; D010935:Plant Diseases / Q000821:virology*; D010937:Plant Growth Regulators / Q000378:metabolism / Q000494:pharmacology; D018515:Plant Leaves / Q000821:virology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D017863:Potexvirus / Q000235:genetics / Q000472:pathogenicity*; D017800:Potyvirus / Q000235:genetics / Q000472:pathogenicity*; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics* / Q000821:virology; D014027:Tobacco Mosaic Virus / Q000235:genetics*; D059467:Transcriptome; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D010937:Plant Growth Regulators; D010940:Plant Proteins; D014764:Viral Proteins; D003546:Cysteine Endopeptidases; C072775:HC-Pro protein, potyvirus; D046988:Proteasome Endopeptidase Complex