title: Synthesis, molecular docking, and biofilm formation inhibitory activity of 5-substituted 3,4-dihalo-5h-furan-2-one derivatives on Pseudomonas aeruginosa.
abstract: Pseudomonas aeruginosa (P. aeruginosa) colonize on most wounds and live as biofilm, which causes antibiotic resistance and wounds unhealed. To investigate the effects of 5-substituted 3,4-dihalo-5H-furan-2-one compounds on biofilm formation of P. aeruginosa, a set of 5-(aryl-1'-hydroxy-methyl)- or 5-(aryl-2-methylene)-3,4-dihalo-5H-furan-2-one compounds were designed and synthesized. Their inhibitory activities on biofilm formation of P. aeruginosa were studied by MIC assay, quantitative analysis of biofilm inhibition, and observation of biofilm formation with SEM. It was found that compounds 2i, 3f, 3i showed remarkable effects of biofilm formation inhibition on P. aeruginosa. Furthermore, molecular docking was performed to identify the key structural features of these compounds with the binding site of LasR receptor.
authors.affiliation: Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, College of Pharmacy, Jinan University, Guangzhou, China.
authors.forename: Guo-Yong
authors.initials: GY
authors.lastname: Liu
authors.forename: Bao-Qin
authors.initials: BQ
authors.lastname: Guo
authors.forename: Wan-Na
authors.initials: WN
authors.lastname: Chen
authors.forename: Chao
authors.initials: C
authors.lastname: Cheng
authors.forename: Qian-Lan
authors.initials: QL
authors.lastname: Zhang
authors.forename: Mi-Bei
authors.initials: MB
authors.lastname: Dai
authors.forename: Jun-Rong
authors.initials: JR
authors.lastname: Sun
authors.forename: Ping-Hua
authors.initials: PH
authors.lastname: Sun
authors.forename: Wei-Min
authors.initials: WM
authors.lastname: Chen
mesh_terms: D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D001665:Binding Sites; D018441:Biofilms / Q000187:drug effects*; D005663:Furans / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D006801:Humans; D008958:Models, Molecular; D011552:Pseudomonas Infections / Q000188:drug therapy / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D015534:Trans-Activators / Q000737:chemistry / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D005663:Furans; C080365:LasR protein, Pseudomonas aeruginosa; D015534:Trans-Activators; C039281:furan