title: Enhancement of crystallization with nucleotide ligands identified by dye-ligand affinity chromatography.
abstract: Ligands interacting with Mycobacterium tuberculosis recombinant proteins were identified through use of the ability of Cibacron Blue F3GA dye to interact with nucleoside/nucleotide binding proteins, and the effects of these ligands on crystallization were examined. Co-crystallization with ligands enhanced crystallization and enabled X-ray diffraction data to be collected to a resolution of atleast 2.7 Å for 5 of 10 proteins tested. Additionally, clues about individual proteins’ functions were obtained from their interactions with each of a panel of ligands.
authors.affiliation: Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA.
authors.forename: Heungbok
authors.initials: H
authors.lastname: Kim
authors.forename: Cecelia
authors.initials: C
authors.lastname: Webster
authors.forename: Justin K M
authors.initials: JK
authors.lastname: Roberts
authors.forename: Juthamas
authors.initials: J
authors.lastname: Kositsawat
authors.forename: Li-Wei
authors.initials: LW
authors.lastname: Hung
authors.forename: Thomas C
authors.initials: TC
authors.lastname: Terwilliger
authors.forename: Chang-Yub
authors.initials: CY
authors.lastname: Kim
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D002846:Chromatography, Affinity / Q000379:methods*; D018360:Crystallography, X-Ray / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics; D008024:Ligands; D009169:Mycobacterium tuberculosis / Q000737:chemistry / Q000235:genetics; D009250:NADP Transhydrogenases / Q000737:chemistry / Q000235:genetics; D009711:Nucleotides / Q000737:chemistry* / Q000235:genetics; D025941:Protein Interaction Mapping / Q000379:methods; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D014227:Triazines / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D008024:Ligands; D009711:Nucleotides; D011994:Recombinant Proteins; D014227:Triazines; C012842:Cibacron Blue F 3GA; D009250:NADP Transhydrogenases