title: Identification of immunogenic proteins of Flavobacterium columnare by two-dimensional electrophoresis immunoblotting with antibacterial sera from grass carp, Ctenopharyngodon idella (Valenciennes).
abstract: Flavobacterium columnare is a Gram-negative bacterium causing columnaris disease of freshwater fish worldwide, and development of efficacious vaccines has been a continuous challenge in aquaculture. In this study, 14 proteins were identified from cellular components of F. columnare using an immunoblotting approach in two-dimensional electrophoresis map gels with antibacterial sera from grass carp, Ctenopharyngodon idella (Valenciennes), and then anti-grass carp-recombinant Ig (rIg) polyclonal antibodies. These proteins were characterized conclusively by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF/TOF MS). The 14 proteins are immunogenic molecules of F. columnare, including chaperonins DnaK, GroEL and trigger factor, and translation elongation factor G, translation elongation factor Tu, 30S ribosomal subunit protein S1, dihydrolipoamide succinyltransferase, succinyl-CoA synthetase, SpoOJ regulator protein, alcohol dehydrogenase, fructose-bisphosphate aldolase, 3-hydroxybutyryl-CoA dehydrogenase and two conserved hypothetical proteins. These identified immunogenic proteins may provide candidate molecules for the development of vaccines against columnaris disease.
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, China College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei Province, China.
authors.forename: Z X
authors.initials: ZX
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, China College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei Province, China.
authors.forename: G Y
authors.initials: GY
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, China College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei Province, China.
authors.forename: N
authors.initials: N
authors.lastname: Li
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, China College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei Province, China.
authors.forename: F S
authors.initials: FS
authors.lastname: Xiao
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, China College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei Province, China.
authors.forename: H X
authors.initials: HX
authors.lastname: Xie
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, China College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei Province, China.
authors.forename: P
authors.initials: P
authors.lastname: Nie
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017756:Aquaculture; D001426:Bacterial Proteins / Q000276:immunology* / Q000302:isolation & purification; D002347:Carps / Q000276:immunology*; D015180:Electrophoresis, Gel, Two-Dimensional; D005393:Fish Diseases / Q000276:immunology / Q000382:microbiology*; D045826:Flavobacteriaceae Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D005417:Flavobacterium / Q000276:immunology*; D007106:Immune Sera; D015151:Immunoblotting; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001426:Bacterial Proteins; D007106:Immune Sera