title: Live-attenuated measles virus vaccine confers cell contact loss and apoptosis of ovarian cancer cells via ROS-induced silencing of E-cadherin by methylation.
abstract: Herein we present a novel molecular mechanism of the antitumor effects of live-attenuated measles virus (MV) vaccine in ovarian cancer. Using a 2-DE/MS-based comparative proteomics strategy, we identified 17 proteins differentially expressed in live-attenuated MV vaccine-treated SKOV-3 ovarian cancer cells, including oxidative stress-associated enzymes and cell contact-related proteins, which indicated that live-attenuated MV vaccine could induce aberrant ROS activation. It further mediated epigenetic silencing of E-cadherin via upregulating DNMT3a that conferred both cell-cell and cell-matrix contact loss and apoptosis of ovarian cancer cells. This process could be reversed through ROS inhibition. Our study lays the theoretical foundation for the clinical application of live-attenuated MV vaccine as a potential oncotherapeutic agent for ovarian cancer treatment.
authors.affiliation: Department of Gynecology and Obstetrics, Key Laboratory of Obstetrics & Gynecologic and Pediatric Diseases and Birth Defects, Ministry of Education, West China Second Hospital, Sichuan University, Chengdu, PR China.
authors.forename: Shengtao
authors.initials: S
authors.lastname: Zhou
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Li
authors.forename: Fuqiang
authors.initials: F
authors.lastname: Huang
authors.forename: Boya
authors.initials: B
authors.lastname: Zhang
authors.forename: Tao
authors.initials: T
authors.lastname: Yi
authors.forename: Zhengyu
authors.initials: Z
authors.lastname: Li
authors.forename: Hong
authors.initials: H
authors.lastname: Luo
authors.forename: Xiang
authors.initials: X
authors.lastname: He
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhong
authors.forename: Ce
authors.initials: C
authors.lastname: Bian
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Lin
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Qi
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.forename: Canhua
authors.initials: C
authors.lastname: Huang
authors.forename: Xia
authors.initials: X
authors.lastname: Zhao
authors.forename: Yuquan
authors.initials: Y
authors.lastname: Wei
mesh_terms: D017209:Apoptosis; D015153:Blotting, Western; D015820:Cadherins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D002448:Cell Adhesion; D002465:Cell Movement; D049109:Cell Proliferation; D019175:DNA Methylation; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005455:Fluorescent Antibody Technique; D020868:Gene Silencing; D006801:Humans; D008458:Measles Vaccine / Q000627:therapeutic use*; D010051:Ovarian Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000628:therapy*; D012333:RNA, Messenger / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D021241:Spectrometry, Mass, Electrospray Ionization; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays
chemical_list: D015820:Cadherins; D008458:Measles Vaccine; D012333:RNA, Messenger; D017382:Reactive Oxygen Species