title: Inhibition of macrophage oxidative stress prevents the reduction of ABCA-1 transporter induced by advanced glycated albumin.
abstract: We investigated the role of aminoguanidine and benfotiamine on the inhibition of reactive oxygen species (ROS) generation in macrophages induced by advanced glycated albumin (AGE-albumin) and its relationship with cell cholesterol homeostasis, emphasizing the expression of the ATP binding cassette transporter A-1 (ABCA-1). AGE-albumin was made by incubating fatty acid-free albumin with 10 mM glycolaldehyde. ROS production and ABCA-1 protein level were determined by flow cytometry in J774 macrophages treated along time with control (C) or AGE-albumin alone or in the presence of aminoguanidine or benfotiamine. Mitochondrial function was evaluated by oxygraphy. Compared to C-albumin, AGE-albumin increased ROS production in macrophages, which was ascribed to the activities of NADPH oxidase and of the mitochondrial system. Mitochondrial respiratory chain activity was reduced in cells incubated with AGE-albumin. ROS generation along time was associated with the reduction in macrophage ABCA-1 protein level. Aminoguanidine prevented ROS elevation and restored the ABCA-1 content in macrophages; on the other hand, benfotiamine that promoted a lesser reduction in ROS generation was not able to restore ABCA-1 levels. Inhibition of oxidative stress induced by AGE-albumin prevents disturbances in reverse cholesterol transport by curbing the reduction of ABCA-1 elicited by advanced glycation in macrophages and therefore may contribute to the prevention of atherosclerosis in diabetes mellitus.
authors.affiliation: Lipids Laboratory-LIM 10, Faculty of Medical Sciences, University of São Paulo, Av. Dr Arnaldo 455-Room 3305, São Paulo 01246-000, Brazil.
authors.forename: Raphael
authors.initials: R
authors.lastname: de Souza Pinto
authors.forename: Gabriela
authors.initials: G
authors.lastname: Castilho
authors.forename: Bruno Alves
authors.initials: BA
authors.lastname: Paim
authors.forename: Adriana
authors.initials: A
authors.lastname: Machado-Lima
authors.forename: Natalia M
authors.initials: NM
authors.lastname: Inada
authors.forename: Edna Regina
authors.initials: ER
authors.lastname: Nakandakare
authors.forename: Aníbal Eugênio
authors.initials: AE
authors.lastname: Vercesi
authors.forename: Marisa
authors.initials: M
authors.lastname: Passarelli
mesh_terms: D064286:ATP Binding Cassette Transporter 1; D018528:ATP-Binding Cassette Transporters / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002478:Cells, Cultured; D017127:Glycation End Products, Advanced / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D006146:Guanidines / Q000494:pharmacology; D006801:Humans; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012709:Serum Albumin / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D000075462:Serum Albumin, Human; D013831:Thiamine / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: D064286:ATP Binding Cassette Transporter 1; D018528:ATP-Binding Cassette Transporters; D000975:Antioxidants; D017127:Glycation End Products, Advanced; D006146:Guanidines; D017382:Reactive Oxygen Species; D012709:Serum Albumin; C109748:advanced glycation end products-human serum albumin; C004479:pimagedine; D013831:Thiamine; C013835:benphothiamine; D000075462:Serum Albumin, Human