title: Evaluation of a low cost cryopreservation system on the biology of human amniotic fluid-derived mesenchymal stromal cells.
abstract: BACKGROUND
  Human amniotic-derived mesenchymal stromal cells (hAMSC) are a novel population of multipotent stem cells that have been shown to have great potential for use in regenerative medicine. However, procedures to store and preserve hAMSC for future clinical applications have not been explored extensively.
  
  
  METHODS
  In this study, we analyzed the influence of cryopreservation, using a protocol based on freezing rate of 1 °C/min, 10% dimethyl sulfoxide as cryoprotectant and a thawing rate >100 °C/min, on hAMSC morphology, proliferation rates, viability, cell cycle, karyotype, immune phenotype and multilineage differentiation potential.
  
  
  RESULTS
  This study found that this cryopreservation protocol does not affect the biological properties of hAMSC.
  
  
  CONCLUSIONS
  This shows that this protocol is a viable system for banking hAMSC, with the associated advantages that has a low cost in terms of expense, time and personnel involved and is easy to implement.
authors.affiliation: Department of Immunology, Carlos Haya Regional University Hospital, Malaga 29010, Spain. josem.miranda.exts@juntadeandalucia.es
authors.forename: Jose Maria
authors.initials: JM
authors.lastname: Miranda-Sayago
authors.forename: Nieves
authors.initials: N
authors.lastname: Fernandez-Arcas
authors.forename: Carmen
authors.initials: C
authors.lastname: Benito
authors.forename: Armando
authors.initials: A
authors.lastname: Reyes-Engel
authors.forename: Jose Ramon
authors.initials: JR
authors.lastname: Herrero
authors.forename: Antonio
authors.initials: A
authors.lastname: Alonso
mesh_terms: D000653:Amniotic Fluid / Q000166:cytology* / Q000187:drug effects; D018070:Biological Specimen Banks; D002452:Cell Count; D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D015925:Cryopreservation / Q000191:economics / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D006801:Humans; D016130:Immunophenotyping; D007621:Karyotyping; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects
chemical_list: D003451:Cryoprotective Agents; D004121:Dimethyl Sulfoxide