title: A fraction of neurofibromin interacts with PML bodies in the nucleus of the CCF astrocytoma cell line.
abstract: Neurofibromatosis type 1 is a common genetic disease that causes nervous system tumors, and cognitive deficits. It is due to mutations within the NF1 gene, which encodes the Nf1 protein. Nf1 has been shown to be involved in the regulation of Ras, cAMP and actin cytoskeleton dynamics. In this study, using immunofluorescence experiments, we have shown a partial nuclear localization of Nf1 in the astrocytoma cell line: CCF and we have demonstrated that Nf1 partially colocalizes with PML (promyelocytic leukemia) nuclear bodies. A direct interaction between Nf1 and the multiprotein complex has further been demonstrated using "in situ" proximity ligation assay (PLA).
authors.affiliation: Centre de Biophysique Moléculaire, Centre National de la Recherche Scientifique (CNRS), UPR 4301, Université d'Orléans et INSERM, rue Charles Sadron, 45071 Orléans Cedex 2, France.
authors.forename: Fabienne
authors.initials: F
authors.lastname: Godin
authors.forename: Sandrine
authors.initials: S
authors.lastname: Villette
authors.forename: Béatrice
authors.initials: B
authors.lastname: Vallée
authors.forename: Michel
authors.initials: M
authors.lastname: Doudeau
authors.forename: Séverine
authors.initials: S
authors.lastname: Morisset-Lopez
authors.forename: Maryvonne
authors.initials: M
authors.lastname: Ardourel
authors.forename: Tobias
authors.initials: T
authors.lastname: Hevor
authors.forename: Chantal
authors.initials: C
authors.lastname: Pichon
authors.forename: Hélène
authors.initials: H
authors.lastname: Bénédetti
mesh_terms: D001253:Astrocytes / Q000201:enzymology*; D001254:Astrocytoma / Q000201:enzymology; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000201:enzymology*; D006801:Humans; D046912:Multiprotein Complexes / Q000378:metabolism; D025542:Neurofibromin 1 / Q000378:metabolism*
chemical_list: D046912:Multiprotein Complexes; D025542:Neurofibromin 1