title: [Approaches towards directed DNA integration by the use of retroviral integrases and transposases].
abstract: The ability of retroviruses and transposases to insert own genome into a host-cell allow us to consider them as a preferable object for constructing gene therapy vectors. However, enzymes that perform the insertion of the genetic material do not display a selectivity towards target nucleotide sequences that results in an almost random DNA introduction into the recipient cell genome. Random insertion leads to mutations which might cause a number of undesirable consequences including neoplastic transformation in the cell. Thereby, in order to achieve a successful functioning of retroviral and trasposonal genetic therapy systems, it is essential to modify them in such a way that directed integration of the vector in a target sequence in the human genome could be achieved. In the review approaches that have been developed for a high specific modification of genome, including the construction of hybrid proteins on the basis of retroviral integrases, transposases, as well as cellular factors interacting with these enzymes, are presented.
authors.forename: E S
authors.initials: ES
authors.lastname: Kniazhanskaia
authors.forename: O V
authors.initials: OV
authors.lastname: Kondrashina
authors.forename: M B
authors.initials: MB
authors.lastname: Gottikh
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods*; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000235:genetics*; D015894:Genome, Human; D015497:HIV-1 / Q000235:genetics; D006801:Humans; D019426:Integrases / Q000235:genetics / Q000378:metabolism*; D016254:Mutagenesis, Insertional; D012190:Retroviridae / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics; D019895:Transposases / Q000235:genetics / Q000378:metabolism*; D016662:Virus Integration / Q000235:genetics*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C504649:PSIP1 protein, human; D014157:Transcription Factors; D019426:Integrases; D019895:Transposases