Helicobacter pylori in Thai patients with cholangiocarcinoma and its association with biliary inflammation and proliferation.
OBJECTIVE
To investigate whether Helicobacter spp. infection and the cagA of H. pylori are associated with hepatobiliary pathology, specifically biliary inflammation, cell proliferation and cholangiocarcinoma (CCA).


METHODS
Helicobacter species including H. pylori, H. bilis and H. hepaticus were detected in the specimens using the polymerase chain reaction (PCR). Biliary inflammation of the liver and gallbladders was semi-quantitatively graded on hematoxylin and eosin (H&E)-stained slides. Biliary proliferation was evaluated by immunohistochemistry using the Ki-67-labelling index.


RESULTS
Helicobacter pylori was found in 66.7%, 41.5% and 25.0% of the patients in the CCA, cholelithiasis and control groups (P < 0.05), respectively. By comparison, H. bilis was found in 14.9% and 9.4% of the patients with CCA and cholelithiasis, respectively (P > 0.05), and was absent in the control group. The cagA gene of H. pylori was detected in 36.2% and 9.1% of the patients with CCA and cholelithiasis, respectively (P < 0.05). Among patients with CCA, cell inflammation and proliferation in the liver and gallbladder were significantly higher among those DNA H. pylori positive than negative.


CONCLUSIONS
The present findings suggest that H. pylori, especially the cagA-positive strains, may be involved in the pathogenesis of hepatobiliary diseases, especially CCA through enhanced biliary cell inflammation and proliferation.
Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Wongwarut
W
Boonyanugomol
Chariya
C
Chomvarin
Banchob
B
Sripa
Vajarabhongsa
V
Bhudhisawasdi
Narong
N
Khuntikeo
Chariya
C
Hahnvajanawong
Amporn
A
Chamsuwan
D000942:Antigens, Bacterial / Q000235:genetics; D044466:Asian People; D001426:Bacterial Proteins / Q000235:genetics; D001650:Bile Duct Neoplasms / Q000737:chemistry / Q000208:ethnology / Q000382:microbiology* / Q000473:pathology; D001653:Bile Ducts, Intrahepatic / Q000737:chemistry / Q000382:microbiology* / Q000473:pathology; D016022:Case-Control Studies; D049109:Cell Proliferation; D016009:Chi-Square Distribution; D018281:Cholangiocarcinoma / Q000737:chemistry / Q000208:ethnology / Q000382:microbiology* / Q000473:pathology; D002761:Cholangitis / Q000208:ethnology / Q000382:microbiology* / Q000473:pathology; D016481:Helicobacter Infections / Q000208:ethnology / Q000382:microbiology*; D044923:Helicobacter hepaticus / Q000302:isolation & purification; D016480:Helicobacter pylori / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000032:analysis; D016133:Polymerase Chain Reaction; D013785:Thailand / Q000453:epidemiology
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D019394:Ki-67 Antigen; C080866:cagA protein, Helicobacter pylori