Palynological origin, phenolic content, and antioxidant properties of honeybee-collected pollen from Bahia, Brazil.
The aim of this study was to determine the palynological origin, phenolic and flavonoid content, and antioxidant properties of twenty-five samples of bee pollen harvested during a nine-month period (February-November) from the Canavieiras municipality (northeastern Brazil). Of the 25 samples analyzed, only two (February 01 and 02) were heterofloral. The predominant pollens in the samples analyzed during that month were: Cecropia, Eucalyptus, Elaeis, Mimosa pudica, Eupatorium, and Scoparia. Ethyl acetate fractions were analyzed by HPLC-DAD. The flavonoids isoquercetin, myricetin, tricetin, quercetin, luteolin, selagin, kaempferol, and isorhamnetin were detected. The flavonoid present in all 22 samples was isolated and identified as isorhamnetin 3-O-b-neohesperidoside. The total phenolic contents determined using the Folin-Ciocalteu reagent ranged from 41.5 to 213.2 mg GAE/g. Antioxidant activities based on the 1,1-diphenyl-2-picryl hydrazyl (DPPH), 2,2-azinobis 3-ethylbenzothiozoline-6-sulfonic acid (ABTS), and Fe2+ ion chelating activity assays were observed for all extracts, and correlated with the total phenolic content.
Laboratório de Tecnologia Farmaceutica, Universidade Federal da Paraiba, Paraiba, Brazil.
Kristerson R L
KR
Freire
Antonio C S
AC
Lins
Marcos C
MC
Dórea
Francisco A R
FA
Santos
Celso A
CA
Camara
Tania M S
TM
Silva
D000818:Animals; D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology*; D001516:Bees; D001938:Brazil; D002851:Chromatography, High Pressure Liquid; D010636:Phenols / Q000032:analysis*; D011058:Pollen / Q000737:chemistry*
D000975:Antioxidants; D010636:Phenols