Biodistribution and stability of CdSe core quantum dots in mouse digestive tract following per os administration: advantages of double polymer/silica coated nanocrystals.
CdSe-core, ZnS-capped semiconductor quantum dots (QDs) are of great potential for biomedical applications. However, applications in the gastrointestinal tract for in vivo imaging and therapeutic purposes are hampered by their sensitivity to acidic environments and potential toxicity. Here we report the use of coatings with a combination of polythiol ligands and silica shell (QDs PolyT-APS) to stabilize QDs fluorescence under acidic conditions. We demonstrated the stability of water-soluble QDs PolyT-APS both in vitro, in strong acidic solutions, and in vivo. The biodistribution, stability and photoluminescence properties of QDs in the gastrointestinal tract of mice after per os administration were assessed. We demonstrated that QDs coated with current traditional materials - mercapto compounds (QDs MPA) and pendant thiol group (QDs PolyT) - are not capable of protecting QDs from chemically induced degradation and surface modification. Polythiol ligands and silica shell quantum dots (QDs PolyT-APS) are suitable for biological and biomedical applications in the gastrointestinal tract.
Laboratory of Physical Biochemistry, AN Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow 119071, Russian Federation.
Y F
YF
Loginova
S V
SV
Dezhurov
V V
VV
Zherdeva
N I
NI
Kazachkina
M S
MS
Wakstein
A P
AP
Savitsky
D000284:Administration, Oral; D000818:Animals; D019187:Cadmium Compounds / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D020099:Coated Materials, Biocompatible / Q000493:pharmacokinetics*; D005260:Female; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000493:pharmacokinetics*; D041981:Gastrointestinal Tract / Q000378:metabolism*; D008024:Ligands; D008099:Liver / Q000378:metabolism*; D051379:Mice; D008819:Mice, Nude; D010179:Pancreas / Q000378:metabolism*; D045663:Quantum Dots; D018036:Selenium Compounds / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D012822:Silicon Dioxide / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry; D014018:Tissue Distribution
D019187:Cadmium Compounds; D020099:Coated Materials, Biocompatible; D005456:Fluorescent Dyes; D008024:Ligands; D018036:Selenium Compounds; D013438:Sulfhydryl Compounds; D012822:Silicon Dioxide; C058667:cadmium selenide