Utility of a set of conserved mitochondrial cytochrome oxidase subunit I gene primers for Mansonia annulata identification.
DNA-based identification system using the mitochondrial cytochrome oxidase subunit I (COI) gene has enabled validation of many species in certain taxonomic groups. These primer combinations were able to work universally across Insecta. Here, a set of three primer pairs were successful in amplifying COI of Mansonia annulata mosquito, a potential vector of Brugia malayi. By merging all three amplicons the whole COI was obtained. Primer pair TY-J-1460/C1N2087 amplified 5' region of COI, LepF1/LepR1 the central and C1J2090/TL2N3014 the 3' region, generating COI amplicons of 650, 700 and 950 base pairs, respectively. When Ma. annulata sequences were compared with those from online sources, they formed a cluster group that is clearly distinct from other allied species.
Department of Medical Entomology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Chalalai
C
Rueanghiran
Chamnarn
C
Apiwathnasorn
Pradit
P
Sangthong
Yudthana
Y
Samung
Jiraporn
J
Ruangsittichai
D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence; D009033:Culicidae / Q000235:genetics*; D017931:DNA Primers; D004272:DNA, Mitochondrial; D003576:Electron Transport Complex IV / Q000235:genetics*; D050259:Genes, Mitochondrial / Q000235:genetics; D019476:Insect Proteins / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction
D017931:DNA Primers; D004272:DNA, Mitochondrial; D019476:Insect Proteins; D003576:Electron Transport Complex IV