TRESK gene recombinant adenovirus vector inhibits capsaicin-mediated             substance P release from cultured rat dorsal root ganglion neurons.
The present study was conducted to determine whether the activation of TRESK             in the dorsal root ganglion (DRG) by the TRESK gene recombinant adenovirus vector             inhibits the capsaicin-evoked substance P (SP) release using a radioimmunoassay.             TRESK is an outwardly rectifying K+ current channel that contributes to the resting             potential and is the most important background potassium channel in DRG. Previous             studies have shown that neuropathic pain (NP) is closely related to the regulation             of certain potassium channels in DRG neurons, while DRG-released SP is important             in the peripheral mechanism of NP. In the present study, the TRESK gene adenovirus             vector significantly enhanced the TRESK mRNA and protein of the cultured rat DRG             neurons. Radioimmunoassay analysis revealed that the capsaicin‑mediated SP release             was significantly inhibited by the TRESK gene recombinant adenovirus vector in             rat DRG neurons. These findings suggest that TRESK plays a role in adjusting the             release of SP in DRG, which is related to NP.
Department of Anesthesiology, First People's Hospital of Foshan, Foshan, Guangdong, PR China. zhoujun7843@126.com
Jun
J
Zhou
Shang-Long
SL
Yao
Cheng-Xiang
CX
Yang
Ji-Ying
JY
Zhong
Han-Bing
HB
Wang
Yan
Y
Zhang
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D000982:Antipruritics / Q000494:pharmacology*; D002211:Capsaicin / Q000494:pharmacology*; D002478:Cells, Cultured; D005727:Ganglia, Spinal / Q000166:cytology / Q000187:drug effects*; D005822:Genetic Vectors; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D015221:Potassium Channels / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013373:Substance P / Q000378:metabolism*
D000982:Antipruritics; C546314:Kcnk18 protein, rat; D015221:Potassium Channels; D013373:Substance P; D002211:Capsaicin