[The content of nitrite and N-nitroso compounds of plasma as a diagnostic test of nonspecific inflammation].
The authors' enzymatic sensor was applied to identify the content of nitrite and nitroso compounds of blood plasma in normal condition and under various inflammatory diseases. It is established that in normal conditions blood plasma contains nitrite, N-nitroso compounds (RNNO) and S-nitroso compounds (RSNO) in concentrations less than 100 nm. The plasma pool of nitroso compounds includes basically thiolferous nitrosate ferrum complex in concentration 3-20 microm. The concentration of nitrite in plasma is from 10 to 150 microm. The concentration (nitrite+RNNO) increases dramatically under inflammatory diseases. This indicator was 0.3-1.0 microm in examined patients with acute and chronic pancreatitis, cholecystitis, ENT diseases of inflammatory character and ARD. In the case of acute appendicitis the indicator reached 10 micro. In case of successful treatment the content (nitrite+RNNO) decreased to the concentration lower than 100 nm. The content of other nitrite and N-nitroso compounds had no reliable variations. Because of this, largely implemented evaluation of intensity of nitric oxide production by the aggregate indicator of nitrites content (NO(x)) in blood seems ambiguous. The reason is that in normal conditions nitrite is presented in trace amount and considerable quantity of nitrate can enter the organism in an exogenous way. Besides that the content of nitrite can depend on kidneys filterability. At the same time, based on the obtained data, the content (nitrite+RNNO) in plasma in concentrations higher than 150 nm are definitely to be considered as pathology.
V Iu
VIu
Titov
A V
AV
Ivanova
M A
MA
Agapov
V A
VA
Petrov
D015374:Biosensing Techniques; D002374:Catalase / Q000037:antagonists & inhibitors / Q000737:chemistry; D002764:Cholecystitis / Q000097:blood / Q000175:diagnosis; D006801:Humans; D007249:Inflammation / Q000097:blood / Q000175:diagnosis*; D009573:Nitrites / Q000097:blood*; D009603:Nitroso Compounds / Q000097:blood*; D010038:Otorhinolaryngologic Diseases / Q000097:blood / Q000175:diagnosis; D010195:Pancreatitis / Q000097:blood / Q000175:diagnosis; D012128:Respiratory Distress Syndrome / Q000097:blood / Q000175:diagnosis
D009573:Nitrites; D009603:Nitroso Compounds; D002374:Catalase