Evaluating the efficacy of subcellular fractionation of blast cells using live cell labeling and 2D DIGE.
Labeling of exposed cell surface proteins of live cells using CyDye DIGE fluor minimal dyes is an efficient strategy for cell surface proteome profiling and quantifying differentially expressed proteins in diseases. Here we describe a strategy to evaluate a two-step detergent-based protein fractionation method using live cell labeling followed by visualization of the fluorescently labeled cell surface proteins and fractionated proteins within a single 2D gel.
Adelaide Proteomics Centre, University of Adelaide, Adelaide, Australia.
Yin Ying
YY
Ho
Megan
M
Penno
Michelle
M
Perugini
Ian
I
Lewis
Peter
P
Hoffmann
D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D002232:Carbocyanines / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D002470:Cell Survival; D004396:Coloring Agents / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D007091:Image Processing, Computer-Assisted; D042541:Intracellular Space / Q000378:metabolism*; D040901:Proteomics; D013194:Staining and Labeling / Q000379:methods*; D057825:Two-Dimensional Difference Gel Electrophoresis / Q000379:methods*
D002232:Carbocyanines; D004396:Coloring Agents