[Genetic diversity of Staphylococcus aureus strains that colonize nose mucous membrane of children living in a large industrial city].
OBJECTIVE
Study of genetic diversity of Staphylococcus aureus strains that colonize nose mucous membrane of children living in an ecologically unfavorable district of Krasnoyarsk.


METHODS
20 S.aureus strains isolated from mucous membrane of anterior part of nose of children were genotyped by RAPD-PCR (random ampliphied polymorphic DNA) with an oligonucleotide primer (10 Sh1 nucleotide).


RESULTS
Presence of 6 genotypes, with 3 of those including 3 and more strains, was detected among the studied strains. The extent of genetic similarity between genotype 1 and 2 was established at 85%.


CONCLUSIONS
20 of 63 (31.7%) of examined students were resident carriers of S. aureus. 3 main genotypes of S. aureus that circulate in students were detected by RAPD-PCR. This confirms the necessity to use RAPD-PCR method for timely evaluation of epidemic situation in organized groups of children.
S S
SS
Baksheeva
L P
LP
Avetisian
M Iu
MIu
Chernukha
I A
IA
Shaginian
D015373:Bacterial Typing Techniques; D002648:Child; D017931:DNA Primers / Q000737:chemistry* / Q000235:genetics; D004269:DNA, Bacterial / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D004787:Environmental Pollution; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008297:Male; D009297:Nasal Mucosa / Q000382:microbiology*; D009666:Nose / Q000382:microbiology*; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D012800:Siberia; D013203:Staphylococcal Infections / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
D017931:DNA Primers; D004269:DNA, Bacterial