Single-cell culture in microwells.
In order to better understand cellular processes and behavior, a controlled way of studying high numbers of single cells and their clone formation is greatly needed. This chapter describes a microwell plate with 672 wells in a standard array/slide format, applied for single-cell culture and analysis. Single cells can be seeded into each well of the plate (1) manually or (2) automatically using a sorting flow cytometer, followed by week-long culture and detection of cell growth, protein expression, etc. The glass/silicon plate is compatible with most standard instrumentation to facilitate easy handling and enable use of the plate for single-cell analysis in most laboratory settings.
Department of Cell and Molecular Biology, Science For Life Laboratory, Karolinska Institute, Stockholm, Sweden. sara.lindstrom@ki.se
Sara
S
Lindström
Helene
H
Andersson-Svahn
D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002460:Cell Line; D004867:Equipment Design; D005434:Flow Cytometry / Q000379:methods; D005456:Fluorescent Dyes / Q000032:analysis; D006801:Humans; D059010:Single-Cell Analysis / Q000295:instrumentation* / Q000379:methods
D005456:Fluorescent Dyes