Flow-through pretreatment with strongly acidic electrolyzed water for hemicellulose removal and enzymatic hydrolysis of corn stover.
The application of strongly acidic electrolyzed water (SAEW) in a flow-through system for the treatment of recalcitrant corn stover resulted in enhanced hemicellulose degradation at 160°C and 180°C compared to hydrochloric acid treatment under the same pH. Pretreatment conditions were optimized by varying the four main factors: pH, flow rate, temperature, and reaction time. About 96% of the hemicellulose was removed when corn stover was treated at 180°C for 20min, with SAEW at pH 2.0 and a flow rate of 10mL/min. The recovery of total monomeric and oligomeric xylose in the liquid fraction reached 93%. Using 30 filter paper units of cellulase per gram of cellulose, a digestibility of 100% was achieved under these conditions. Therefore, flow-through SAEW pretreatment is an efficient way to remove hemicellulose and improve enzymatic digestibility of corn stover.
College of Food Science and Nutritional Engineering, China Agricultural University, P.O. Box 398, 17 Qinghua Donglu, Haidian District, Beijing 100083, China.
Haisheng
H
Pei
Li
L
Liu
Xiuqing
X
Zhang
Junshe
J
Sun
D000143:Acids / Q000737:chemistry*; D002482:Cellulose / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D011134:Polysaccharides / Q000302:isolation & purification*; D013696:Temperature; D014867:Water / Q000737:chemistry*; D003313:Zea mays / Q000737:chemistry*
D000143:Acids; D011134:Polysaccharides; D014867:Water; C007916:hemicellulose; D002482:Cellulose