microRNA-155 silencing inhibits proliferation and migration and induces             apoptosis by upregulating BACH1 in renal cancer cells.
The purpose of this study was to explore the functional role and mechanism             of miR-155 in the development of renal cell carcinoma (RCC). miR-155 expression             was quantified in renal cancers, matched adjacent non‑tumor tissues and renal             cell lines using quantitative real-time PCR (RT-PCR). Cell proliferation, apoptosis             and migratory activity were measured following suppression of miR-155 expression             by antisense oligonucleotides. miR-155 targets were scanned using target prediction             programs. Following the inhibition of miR-155, target gene expression was detected             by western blotting. The expression of miR-155 was upregulated in clear cell RCC             (ccRCC) tissue and renal cancer cell lines. The suppression of miR-155 inhibited             cell proliferation and migratory activity and induced apoptosis in renal cancer             cells. The suppressor gene suppressor of cytokine signaling (SOCS-1) and BACH1             were predicted as potential target genes by bioinformatics analysis. The suppression             of miR-155 inhibited BACH1 protein expression. miR-155 may function as an oncogene             by targeting BACH1. Thus, the inhibition of miR-155 may be an effective way to             treat RCC.
Department of Urology, Second Xiangya Hospital, Central South University, Changsha, PR China.
Songchao
S
Li
Tieding
T
Chen
Zhaohui
Z
Zhong
Yi
Y
Wang
Yangle
Y
Li
Xiaokun
X
Zhao
D000368:Aged; D017209:Apoptosis / Q000187:drug effects*; D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics / Q000378:metabolism*; D002292:Carcinoma, Renal Cell / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D051856:Fanconi Anemia Complementation Group Proteins / Q000235:genetics / Q000378:metabolism*; D020868:Gene Silencing; D006801:Humans; D007680:Kidney Neoplasms / Q000378:metabolism* / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D000071222:Suppressor of Cytokine Signaling 1 Protein; D050826:Suppressor of Cytokine Signaling Proteins / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects*
C102108:BACH1 protein, human; D050976:Basic-Leucine Zipper Transcription Factors; D051856:Fanconi Anemia Complementation Group Proteins; C524942:MIRN155 microRNA, human; D035683:MicroRNAs; D016376:Oligonucleotides, Antisense; C108944:SOCS1 protein, human; D000071222:Suppressor of Cytokine Signaling 1 Protein; D050826:Suppressor of Cytokine Signaling Proteins