Overexpression of OsHsp17.0 and OsHsp23.7 enhances drought and salt tolerance in rice.
Heat shock proteins (Hsps) play an important role in plant stress tolerance. We previously reported that expression of OsHsp17.0 and OsHsp23.7 could be enhanced by heat shock treatment and/or other abiotic stresses. In this paper, stress tolerance assays of transgenic rice plants overexpressing OsHsp17.0 and OsHsp23.7 have been carried out. Both OsHsp17.0-OE and OsHsp23.7-OE transgenic lines demonstrated higher germination ability compared to wild-type (WT) plants when subjected to mannitol and NaCl. Phenotypic analysis showed that transgenic rice lines displayed a higher tolerance to drought and salt stress compared to WT plants. In addition, transgenic rice lines showed significantly lower REC, lower MDA content and higher free proline content than WT under drought and salt stresses. These results suggest that OsHsp17.0 and OsHsp23.7 play an important role in rice acclimation to salt and drought stresses and are useful for engineering drought and salt tolerance rice.
Hunan Provincial Key Laboratory for Germplasm Innovation and Utilization of Crop, Hunan Agricultural University, Changsha 410128, China.
Jie
J
Zou
Cuifang
C
Liu
Ailing
A
Liu
Dian
D
Zou
Xinbo
X
Chen
D000222:Adaptation, Physiological / Q000187:drug effects / Q000235:genetics*; D055864:Droughts; D004553:Electric Conductivity; D004573:Electrolytes / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D018525:Germination / Q000187:drug effects; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D008353:Mannitol / Q000494:pharmacology; D012275:Oryza / Q000187:drug effects / Q000235:genetics* / Q000502:physiology*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D011392:Proline / Q000378:metabolism; D055049:Salt Tolerance / Q000187:drug effects / Q000235:genetics*; D012639:Seeds / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics; D013696:Temperature
D004573:Electrolytes; D006360:Heat-Shock Proteins; D010940:Plant Proteins; D008353:Mannitol; D012965:Sodium Chloride; D008315:Malondialdehyde; D011392:Proline