Bioconversion of proposed precursors into theobroxide and related compounds.
We have previously reported a tetraketide origin for theobroxide and its related compound. In the present study, bioconversion of natural and deuterium-labeled precursors of this proposed biosynthetic pathway by Lasiodipoldia theobromae was investigated. Theobroxide was quantified after bioconversion from each proposed precursor. The transformation of the isotopically labeled precursor to products was tracked by 2H NMR measurement.
Laboratory of Bioorganic Chemistry, Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Kita 9, Nishi 9, Kita ku, Sapporo 060-8589, Japan. yolipeng@criepi.denken.or.jp
Peng
P
Li
Kosaku
K
Takahashi
Ahmed
A
Elkhateeb
Hideyuki
H
Matsuura
Teruhiko
T
Yoshihara
Kensuke
K
Nabeta
D001203:Ascomycota / Q000378:metabolism*; D001711:Biotransformation; D003510:Cyclohexanes / Q000378:metabolism*; D003903:Deuterium; D004852:Epoxy Compounds / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy
D003510:Cyclohexanes; D004852:Epoxy Compounds; C496818:theobroxide; D003903:Deuterium