Kordiimonas aestuarii sp. nov., a marine bacterium isolated from a tidal flat.
A Gram-staining negative, strictly aerobic bacterium, designated 101-1(T), was isolated from a sea tidal flat, Taean, Korea. The strain formed small light-yellow, smooth, and circular colonies on marine agar. Cells were weakly halophilic, motile rods showing catalase- and oxidase-positive reactions. Growth of strain 101-1(T) was observed at 15-40 °C (optimum, 30 °C), pH 5.0-8.0 (optimum, pH 6.5-7.0) and 1.0-9.0% (w/v) NaCl (optimum, 2.0-3.5%). The G+C content of the genomic DNA was 53.3 mol%. Strain 101-1(T) contained ubiquinone-10 (Q-10) as the respiratory quinone and iso-C(17:1)ω9c, iso-C(15:0) and iso-C(17:0) as major fatty acids. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain 101-1(T) formed a tight phylogenetic lineage with members of the genus Kordiimonas and was most closely related to Kordiimonas gwangyangensis GW14-5(T) and Kordiimonas lacus S3-22(T) with 97.3% and 96.3% 16S rRNA gene sequence similarities, respectively. The DNA-DNA relatedness values between strain 101-1(T) and K. gwangyangensis GW14-5(T) and K. lacus S3-22(T) were 24.8 ± 4.4% and 32.2 ± 3.6%, respectively. Based on the data from the phenotypic and genotypic studies, strain 101-1(T) represents a novel species of the genus Kordiimonas, for which the name Kordiimonas aestuarii sp. nov. is proposed. The type strain is 101-1(T) ( = KACC 16184(T) = JCM 17742(T)).
School of Biological Sciences & Research Center for Biomolecules and Biosystems, Chung-Ang University, Seoul 156-756, Republic of Korea.
Renukaradhya K
RK
Math
School of Biological Sciences & Research Center for Biomolecules and Biosystems, Chung-Ang University, Seoul 156-756, Republic of Korea.
Sang Hyeon
SH
Jeong
School of Biological Sciences & Research Center for Biomolecules and Biosystems, Chung-Ang University, Seoul 156-756, Republic of Korea.
Hyun Mi
HM
Jin
School of Biological Sciences & Research Center for Biomolecules and Biosystems, Chung-Ang University, Seoul 156-756, Republic of Korea.
Moon Su
MS
Park
School of Biological Sciences & Research Center for Biomolecules and Biosystems, Chung-Ang University, Seoul 156-756, Republic of Korea.
Jeong Myeong
JM
Kim
School of Biological Sciences & Research Center for Biomolecules and Biosystems, Chung-Ang University, Seoul 156-756, Republic of Korea.
Che Ok
CO
Jeon
D020561:Alphaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000032:analysis
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2