Genome-wide analysis of the ATP-binding cassette (ABC) transporter gene family in the silkworm, Bombyx mori.
The ATP-binding cassette (ABC) superfamily is a larger protein family with diverse physiological functions in all kingdoms of life. We identified 53 ABC transporters in the silkworm genome, and classified them into eight subfamilies (A-H). Comparative genome analysis revealed that the silkworm has an expanded ABCC subfamily with more members than Drosophila melanogaster, Caenorhabditis                         elegans, or Homo                         sapiens. Phylogenetic analysis showed that the ABCE and ABCF genes were highly conserved in the silkworm, indicating possible involvement in fundamental biological processes. Five multidrug resistance-related genes in the ABCB subfamily and two multidrug resistance-associated-related genes in the ABCC subfamily indicated involvement in biochemical defense. Genetic variation analysis revealed four ABC genes that might be evolving under positive selection. Moreover, the silkworm ABCC4 gene might be important for silkworm domestication. Microarray analysis showed that the silkworm ABC genes had distinct expression patterns in different tissues on day 3 of the fifth instar. These results might provide new insights for further functional studies on the ABC genes in the silkworm genome.
The Institute of Agricultural and Life Science, Chongqing University, Chongqing 400044, China.
Xiaodong
X
Xie
Tingcai
T
Cheng
Genhong
G
Wang
Jun
J
Duan
Weihuan
W
Niu
Qingyou
Q
Xia
D018528:ATP-Binding Cassette Transporters / Q000096:biosynthesis / Q000145:classification / Q000235:genetics*; D000818:Animals; D012831:Bombyx / Q000235:genetics*; D018432:Drug Resistance, Multiple / Q000235:genetics; D020869:Gene Expression Profiling; D014644:Genetic Variation; D049750:Genome, Insect; D005810:Multigene Family; D010802:Phylogeny; D017433:Protein Structure, Secondary
D018528:ATP-Binding Cassette Transporters