Reproductive and neurobehavioral effects of clothianidin administered to mice in the diet.
Clothianidin was given in the diet to provide levels of 0% (control), 0.003%, 0.006%, and 0.012% from 5 weeks of age of the F(0)  generation to 11 weeks of age of the F(1)  generation in mice. Selected reproductive and neurobehavioral parameters were measured. In exploratory behavior in the F(0)  generation, average time of movement, number of rearing, and rearing time of adult males increased significantly in a dose-related manner. There was no adverse effect of clothianidin on litter size, litter weight, or sex ratio at birth. The average body weight of male and female offspring was increased significantly in a dose-related manner during the early lactation period. With respect to behavioral developmental parameters, swimming head angle at postnatal day (PND) 7 of male offspring was accelerated significantly in a dose-related manner. Negative geotaxis at PND 7 of female offspring was accelerated significantly in a dose-related manner. For movement activity of exploratory behavior in the F(1)  generation, number of rearing of female offspring increased significantly in a dose-related manner. Movement time of adult males increased significantly in a dose-related manner. The dose levels of clothianidin in the present study produced several adverse effects in neurobehavioral parameters in mice. Nevertheless, it would appear that the levels of the actual dietary intake of clothianidin are unlikely to produce adverse effects in humans.
Department of Environmental Health and Toxicology, Tokyo Metropolitan Institute of Public Health, Tokyo, Japan. t-tanaka@poohlover.net
Toyohito
T
Tanaka
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D001835:Body Weight; D004032:Diet; D004305:Dose-Response Relationship, Drug; D005106:Exploratory Behavior / Q000187:drug effects; D005260:Female; D006146:Guanidines / Q000633:toxicity*; D007774:Lactation; D008098:Litter Size; D008297:Male; D051379:Mice; D009043:Motor Activity / Q000187:drug effects*; D000073943:Neonicotinoids; D012098:Reproduction / Q000187:drug effects*; D012744:Sex Ratio; D013844:Thiazoles / Q000633:toxicity*; D018675:Toxicity Tests / Q000379:methods
D006146:Guanidines; D000073943:Neonicotinoids; D013844:Thiazoles; C480342:clothianidin