Isolation of functional dendritic cells from murine kidneys for immunological characterization.
OBJECTIVE
The kidney is a complex organ, requiring the contributions of multiple cell types to perform its various functions. Within this system the dendritic cell has been demonstrated to play a key role in maintaining the immunological balance of the kidney. In this methods paper we aim to identify the best method for isolating murine renal dendritic cells.


METHODS
The efficiency of isolating dendritic cells from enzymatically digested renal parenchyma by density centrifugation, MACS and FACS was compared.


RESULTS
Density centrifugation enriched dendritic cells by only approximately two fold. However, MACS and FACS resulted in a much higher purity (80% versus 95% respectively).


CONCLUSIONS
Although FACS gave the highest purity, MACS is the optimal method for isolating dendritic cells given cost and time factors. Isolation of a homogeneous population of renal dendritic cells will enable the molecular and functional dissection of these cells in both homeostasis and disease models.
University Hospital Bonn, Institute for Experimental Immunology, Bonn, Germany.
Simon Alexander
SA
Teteris
Katharina
K
Hochheiser
Christian
C
Kurts
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002452:Cell Count; D002469:Cell Separation / Q000379:methods; D002499:Centrifugation, Density Gradient; D003713:Dendritic Cells / Q000276:immunology*; D005434:Flow Cytometry; D018189:Immunomagnetic Separation; D007668:Kidney / Q000166:cytology / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL
D015415:Biomarkers