Inhibitory effects of actinidiamide from Actinidia polygama on allergy and inflammation.
Actinidia polygama Max. was subjected to supercritical fluid extraction (SFE), and the resulting ethanol extract of marc (SFEM) was subjected to sequential fractionation with various solvents. Each extract and fraction was assayed for anti-inflammatory effect. The ethyl acetate fraction (EtOAc) contained the highest level (70.8% inhibition) of anti-inflammatory activity. In order to identify the active constituents, the EtOAc fraction was further fractionated by silica gel and ODS column chromatography. By activity-guided fractionation, an active ceramide was identified as the anti-inflammatory component, and its structure was determined by NMR and MS analysis. The novel ceramide was named actinidiamide, and was found significantly to inhibit nitric oxide (NO) production (30.6% inhibition at 1 µg/mL) in lipopolysaccaride (LPS)-stimulated RAW 264.7 cells and β-hexosaminidase release (91.8% inhibition at 1 µg/mL) in IgE-sensitized RBL-2H3 cells. Thus the presence of actinidiamide conveys allergy and inflammation treatment ability to A. polygama.
Department of Oriental Medicine and Processing, College of Life Sciences, Kyung Hee University, Gyeonggi, Korea.
Myun-Ho
MH
Bang
In-Gyeong
IG
Chae
Eun-Ju
EJ
Lee
Nam-In
NI
Baek
Yoon-Su
YS
Baek
Dae-Young
DY
Lee
In-Seon
IS
Lee
Sam-Pin
SP
Lee
Seun-Ah
SA
Yang
D029042:Actinidia / Q000737:chemistry*; D000818:Animals; D018926:Anti-Allergic Agents / Q000302:isolation & purification*; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification*; D002518:Ceramides / Q000302:isolation & purification* / Q000494:pharmacology; D005591:Chemical Fractionation; D006967:Hypersensitivity / Q000188:drug therapy; D007249:Inflammation / Q000188:drug therapy; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D009569:Nitric Oxide / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000737:chemistry; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
D018926:Anti-Allergic Agents; D000893:Anti-Inflammatory Agents; D002518:Ceramides; D008070:Lipopolysaccharides; D010936:Plant Extracts; D009569:Nitric Oxide; D001619:beta-N-Acetylhexosaminidases