The use of small molecule probes to study spatially separated stimulus-induced signaling pathways.
Simultaneous activation of signaling pathways requires dynamic assembly of higher-order protein complexes at the cytoplasmic domains of membrane-associated receptors in a stimulus-specific manner. Here, using the paradigm of cellular activation through cytokine and innate immune receptors, we demonstrate the proof-of-principle application of small molecule probes for the dissection of receptor-proximal signaling processes, such as activation of the transcription factor NF-κB and the protein kinase p38.
Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, 92037 CA, USA.
Vladimir V
VV
Kravchenko
Christian
C
Gloeckner
G Neil
GN
Stowe
Young J
YJ
Kang
Peter S
PS
Tobias
John C
JC
Mathison
Richard J
RJ
Ulevitch
Gunnar F
GF
Kaufmann
Kim D
KD
Janda
D000818:Animals; D002478:Cells, Cultured; D007113:Immunity, Innate / Q000187:drug effects; D008070:Lipopolysaccharides / Q000276:immunology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000276:immunology*; D015398:Signal Transduction / Q000187:drug effects*; D054852:Small Molecule Libraries / Q000737:chemistry / Q000494:pharmacology*; D048051:p38 Mitogen-Activated Protein Kinases / Q000276:immunology*
D008070:Lipopolysaccharides; D016328:NF-kappa B; D054852:Small Molecule Libraries; D048051:p38 Mitogen-Activated Protein Kinases