A chromenoquinoline-based fluorescent off-on thiol probe for bioimaging.
A new chromenoquinoline-based fluorescent off-on thiol probe 2 is reported. In aqueous buffer solutions at physiological pH, the probe exhibited 223-fold enhancement in fluorescence intensity by a Michael addition of cysteine to the maleimide appended to a chromenoquinoline. Cell permeability and live cell imaging of thiols are also demonstrated.
Department of Chemistry, Dmitri Mendeleev Block, Indian Institute of Science Education and Research Pune, India.
Dnyaneshwar
D
Kand
Arunasree Marasanapalli
AM
Kalle
Sreejith Jayasree
SJ
Varma
Pinaki
P
Talukdar
D001578:Benzopyrans / Q000737:chemistry*; D045744:Cell Line, Tumor; D002463:Cell Membrane Permeability; D003545:Cysteine / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D008301:Maleimides / Q000737:chemistry; D008856:Microscopy, Fluorescence; D011789:Quantum Theory; D011804:Quinolines / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry*
D001578:Benzopyrans; D005456:Fluorescent Dyes; D008301:Maleimides; D011804:Quinolines; D013438:Sulfhydryl Compounds; C043592:maleimide; C037219:quinoline; D003545:Cysteine