Stereospecific formation of a ternary complex of (S)-α,β-fluoromethylene-dATP with DNA pol β.
The influence of water: crystallization of (R/S)-α,β-CHF-dATP with the preorganized pol β-DNA complex shows that (S)-α,β-CHF-dATP is preferentially bound to the active site with the C=F fluorine proximal to a structural water bound to Asp276.
Departments of Chemistry and Biology, University of Southern California, Los Angeles, CA 90089, USA.
Brian T
BT
Chamberlain
Vinod K
VK
Batra
William A
WA
Beard
Anastasia P
AP
Kadina
David D
DD
Shock
Boris A
BA
Kashemirov
Charles E
CE
McKenna
Myron F
MF
Goodman
Samuel H
SH
Wilson
D018360:Crystallography, X-Ray; D019951:DNA Polymerase beta / Q000737:chemistry* / Q000378:metabolism; D003838:Deoxyadenine Nucleotides / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D015394:Molecular Structure; D013237:Stereoisomerism
D003838:Deoxyadenine Nucleotides; D019951:DNA Polymerase beta