Effect of a high surface-to-volume ratio on fluorescence-based assays.
In the work discussed in this paper, the effect of a high surface-to-volume ratio of a microfluidic detection cell on fluorescence quenching was studied. It was found that modification of the geometry of a microchannel can provide a wider linear range. This is a phenomenon which should be taken into consideration when microfluidic systems with fluorescence detection are developed. The dependence of the linear range for fluorescein on the surface-to-volume ratio was determined. Both fluorescence inner-filter effects and concentration self-quenching were taken into consideration. It was found that inner-filter effects have little effect on the extent of the linear range on the microscale.
Department of Microbioanalytics, Faculty of Chemistry, Warsaw University of Technology, Warsaw, Poland. r.kwapiszewski@gmail.com
Radoslaw
R
Kwapiszewski
Karina
K
Ziolkowska
Kamil
K
Zukowski
Michal
M
Chudy
Artur
A
Dybko
Zbigniew
Z
Brzozka
D005453:Fluorescence; D005456:Fluorescent Dyes; D044085:Microfluidics
D005456:Fluorescent Dyes