Glucans of Pleurotus florida blue variant: isolation, purification, characterization and immunological studies.
Two different glucans (PS-I and PS-II) were isolated from the alkaline extract of the fruiting bodies of an edible mushroom, Pleurotus florida blue variant and the PS-I showed macrophage, splenocyte and thymocyte activations. On the basis of sugar analysis, methylation analysis, periodate oxidation, and NMR studies ((1)H, (13)C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HMQC and HMBC), the structure of the repeating unit of these polysaccharides were established.
Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
Biswajit
B
Dey
Sanjoy K
SK
Bhunia
Kankan K
KK
Maity
Sukesh
S
Patra
Soumitra
S
Mandal
Swatilekha
S
Maiti
Tapas K
TK
Maiti
Samir R
SR
Sikdar
Syed S
SS
Islam
D000276:Adjuvants, Immunologic / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D002240:Carbohydrate Sequence; D005936:Glucans / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D008969:Molecular Sequence Data; D010858:Pigmentation; D020076:Pleurotus / Q000737:chemistry*; D012995:Solubility; D013154:Spleen / Q000187:drug effects / Q000276:immunology; D060168:Thymocytes / Q000187:drug effects / Q000276:immunology; D014867:Water / Q000737:chemistry
D000276:Adjuvants, Immunologic; D005936:Glucans; D014867:Water