Structural insights into methyltransferase KsgA function in 30S ribosomal subunit biogenesis.
The assembly of the ribosomal subunits is facilitated by ribosome biogenesis factors. The universally conserved methyltransferase KsgA modifies two adjacent adenosine residues in the 3'-terminal helix 45 of the 16 S ribosomal RNA (rRNA). KsgA recognizes its substrate adenosine residues only in the context of a near mature 30S subunit and is required for the efficient processing of the rRNA termini during ribosome biogenesis. Here, we present the cryo-EM structure of KsgA bound to a nonmethylated 30S ribosomal subunit. The structure reveals that KsgA binds to the 30S platform with the catalytic N-terminal domain interacting with substrate adenosine residues in helix 45 and the C-terminal domain making extensive contacts to helix 27 and helix 24. KsgA excludes the penultimate rRNA helix 44 from adopting its position in the mature 30S subunit, blocking the formation of the decoding site and subunit joining. We suggest that the activation of methyltransferase activity and subsequent dissociation of KsgA control conformational changes in helix 44 required for final rRNA processing and translation initiation.
Institute of Molecular Biology and Biophysics, ETH Zurich, 8093 Zurich, Switzerland, and.
Daniel
D
Boehringer
Department of Physiology and Biophysics, Virginia Commonwealth University, Richmond, Virginia 23298.
Heather C
HC
O'Farrell
Department of Physiology and Biophysics, Virginia Commonwealth University, Richmond, Virginia 23298. Electronic address: jason.rife@vcu.edu.
Jason P
JP
Rife
Institute of Molecular Biology and Biophysics, ETH Zurich, 8093 Zurich, Switzerland, and. Electronic address: ban@mol.biol.ethz.ch.
Nenad
N
Ban
D020285:Cryoelectron Microscopy; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008780:Methyltransferases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D010442:Peptide Chain Initiation, Translational / Q000502:physiology; D017434:Protein Structure, Tertiary; D012323:RNA Processing, Post-Transcriptional / Q000502:physiology; D012329:RNA, Bacterial / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012343:RNA, Transfer; D054680:Ribosome Subunits, Small, Bacterial / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
D029968:Escherichia coli Proteins; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012343:RNA, Transfer; D008780:Methyltransferases; C047346:16S rRNA (adenine(1518)-N(6)-adenine(1519)-N(6))-dimethyltransferase