Rapid and simple cryopreservation of anaerobic ammonium-oxidizing bacteria.
A quick and simple protocol for long-term cryopreservation of anaerobic ammonium-oxidizing bacteria (anammox bacteria) was developed. After 29 weeks of preservation at -80°C, activity recovery for all tested cultures under at least one of the applied sets of preservation conditions was observed. Growth recovery was also demonstrated for a single-cell culture of "Candidatus Kuenenia stuttgartiensis."
Ghent University, Department of Biochemistry and Microbiology, Laboratory of Microbiology, Ghent, Belgium. Kim.Heylen@ugent.be
Kim
K
Heylen
Katharina
K
Ettwig
Ziye
Z
Hu
Mike
M
Jetten
Boran
B
Kartal
D000693:Anaerobiosis; D001419:Bacteria / Q000254:growth & development* / Q000378:metabolism; D001431:Bacteriological Techniques / Q000379:methods*; D015925:Cryopreservation / Q000379:methods*; D050296:Microbial Viability; D010084:Oxidation-Reduction; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D013997:Time Factors
D000644:Quaternary Ammonium Compounds