Genetic polymorphism in Taenia solium metacestodes from different Brazilian geographic areas.
The aim of the present study is to investigate genetic polymorphisms in Taenia solium metacestodes from different Brazilian geographical areas and to relate them to antibody recognition in serum samples of neurocysticercosis (NC) patients. Metacestodes were obtained from the Distrito Federal (DF), Bahia, Minas Gerais (MG) and São Paulo (SP) regions of Brazil. Samples of human sera from 49 individuals with NC, 68 individuals with other helminthiasis and 40 healthy volunteers were analysed (157 individuals in total). Antigens were prepared and used in enzyme-linked immunosorbent assay and western blotting assays to detect specific immunoglobulin G antibodies. Genetic distances between metacestode populations were analysed using random amplified polymorphic DNA (RAPD) analysis. Our results show that there was a higher frequency of reactivity in the DF region in the sera from NC patients (p < 0.05), while discrimination between active and inactive NC was seen only in extracts from the MG and SP regions (p < 0.05). Using RAPD, the sample from the DF region presented a greater increase compared to the other regions. A relationship between genetic polymorphisms among T. solium metacestodes from different areas in Brazil and the differences in antibody detection in patients with NC were established.
Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, MG, Brasil.
Ivanildes Solange da Costa
IS
Barcelos
Maria Aparecida
MA
Souza
Janethe Deolinda de Oliveira
JD
Pena
Gleyce Alves
GA
Machado
Lísia Gomes Martins de
LG
Moura
Julia Maria
JM
Costa-Cruz
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000276:immunology*; D015153:Blotting, Western; D001938:Brazil; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D018509:DNA, Helminth / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005843:Geography; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique; D041201:Taenia solium / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D055815:Young Adult
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D018509:DNA, Helminth; D007074:Immunoglobulin G