Discovery of potential diagnostic and vaccine antigens in herpes simplex virus 1 and 2 by proteome-wide antibody profiling.
Routine serodiagnosis of herpes simplex virus (HSV) infections is currently performed using recombinant glycoprotein G (gG) antigens from herpes simplex virus 1 (HSV-1) and HSV-2. This is a single-antigen test and has only one diagnostic application. Relatively little is known about HSV antigenicity at the proteome-wide level, and the full potential of mining the antibody repertoire to identify antigens with other useful diagnostic properties and candidate vaccine antigens is yet to be realized. To this end we produced HSV-1 and -2 proteome microarrays in Escherichia coli and probed them against a panel of sera from patients serotyped using commercial gG-1 and gG-2 (gGs for HSV-1 and -2, respectively) enzyme-linked immunosorbent assays. We identified many reactive antigens in both HSV-1 and -2, some of which were type specific (i.e., recognized by HSV-1- or HSV-2-positive donors only) and others of which were nonspecific or cross-reactive (i.e., recognized by both HSV-1- and HSV-2-positive donors). Both membrane and nonmembrane virion proteins were antigenic, although type-specific antigens were enriched for membrane proteins, despite being expressed in E. coli.
Division of Infectious Diseases, School of Medicine, University of California, Irvine, Irvine, California, USA.
Mina
M
Kalantari-Dehaghi
Sookhee
S
Chun
Aziz Alami
AA
Chentoufi
Jozelyn
J
Pablo
Li
L
Liang
Gargi
G
Dasgupta
Douglas M
DM
Molina
Algis
A
Jasinskas
Rie
R
Nakajima-Sasaki
Jiin
J
Felgner
Gary
G
Hermanson
Lbachir
L
BenMohamed
Philip L
PL
Felgner
D Huw
DH
Davies
D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D016000:Cluster Analysis; D004797:Enzyme-Linked Immunosorbent Assay; D006561:Herpes Simplex / Q000175:diagnosis / Q000453:epidemiology / Q000276:immunology; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000276:immunology*; D018258:Herpesvirus 2, Human / Q000235:genetics / Q000276:immunology*; D022283:Herpesvirus Vaccines / Q000276:immunology; D006801:Humans; D040081:Protein Array Analysis / Q000379:methods; D020543:Proteome / Q000235:genetics / Q000276:immunology*; D012372:ROC Curve; D011994:Recombinant Proteins / Q000276:immunology; D016036:Seroepidemiologic Studies
D000914:Antibodies, Viral; D000956:Antigens, Viral; D022283:Herpesvirus Vaccines; D020543:Proteome; D011994:Recombinant Proteins