In-vitro cytotoxic activity of β-Sitosterol triacontenate isolated from Capparis decidua (Forsk.) Edgew.
OBJECTIVE
To study the isolation and characterization of the constituent responsible for the cytotoxic activity of the ethanolic extract of stem of Capparis decidua (C. decidua).


METHODS
The preliminary cytotoxic effect of isolated compound (β-Sitosterol triacontenate) was investigated by MTT assay on A549 solid tumor cells.


RESULTS
IC(50) value of the β-Sitosterol triacontenate was found to be 1 μM. The cytotoxic activity increased in a dose dependent manner in case of β-Sitosterol triacontenate.


CONCLUSIONS
The data therefore provide direct evidence for the role of β-Sitosterol triacontenate as a potent antimetastatic agent, which can markedly inhibit the metastatic and invasive capacity of malignant cells.
Department of Quality Assurance & Natural Products, PDM College of Pharmacy, Bahadurgarh, India.
Permender
P
Rathee
Dharmender
D
Rathee
Deepti
D
Rathee
Sushila
S
Rathee
D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D031484:Capparis / Q000737:chemistry*; D045744:Cell Line, Tumor; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy*; D009682:Magnetic Resonance Spectroscopy; D009361:Neoplasm Invasiveness / Q000517:prevention & control*; D009362:Neoplasm Metastasis / Q000517:prevention & control*; D010936:Plant Extracts / Q000494:pharmacology*; D018547:Plant Stems / Q000737:chemistry; D012855:Sitosterols / Q000737:chemistry / Q000494:pharmacology*
D000970:Antineoplastic Agents; D010936:Plant Extracts; D012855:Sitosterols; C025473:gamma-sitosterol