BRCA1: a new candidate gene for bovine mastitis and its association analysis between single nucleotide polymorphisms and milk somatic cell score.
Bovine mastitis is a very complex and common disease of dairy cattle and a major source of economic losses to the dairy industry worldwide. In this study, the bovine breast cancer 1, early onset gene (BRCA1) was taken as a candidate gene for mastitis resistance. The main object of this study was to investigate whether the BRCA1 gene was associated with mastitis in cattle. Through DNA sequencing, Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) and Created Restriction Site PCR (CRS-PCR) methods, three SNPs (G22231T, T25025A, and C28300A) were detected and twenty-four combinations of these SNPs were observed. The single SNP and their genetic effects on somatic cell score (SCS) were evaluated and a significant association with SCS was found in C28300A. The mean of genotype EE was significantly lower than those of genotypes EF and FF. The results of combined genotypes analysis of three SNPs showed that BBDDFF genotype with the highest SCS were easily for the mastitis susceptibility, whereas AACCEE genotype with the lowest SCS were favorable for the mastitis resistance. The information provided in the present study will be very useful for improving mastitis resistance in dairy cattle by marker-assisted selection.
Center for Computational Biology and Bioinformatics, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, People's Republic of China.
Zhengrong
Z
Yuan
Guiyan
G
Chu
Yang
Y
Dan
Jiao
J
Li
Lupei
L
Zhang
Xue
X
Gao
Huijiang
H
Gao
Junya
J
Li
Shangzhong
S
Xu
Zhihua
Z
Liu
D000818:Animals; D002417:Cattle; D002452:Cell Count; D060467:Disease Resistance / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D019398:Genes, BRCA1; D056726:Genetic Association Studies; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D005838:Genotype; D008414:Mastitis, Bovine / Q000235:genetics*; D008892:Milk / Q000166:cytology*; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA
D005819:Genetic Markers