The expression of HSPs, anti-oxidants, and cytokines in plasma and bronchoalveolar lavage fluid of patients with acute respiratory distress syndrome.
OBJECTIVE
We studied several acute inflammatory materials (AIM) such as various inflammatory cytokines, oxidative stress, and heat shock proteins in ARDS patients by simultaneously measuring from bronchoalveolar lavage fluid (BALF) and plasma.


METHODS
AIM were measured by using plasma and BALF sampling obtained from ARDS group (n=12) and non-ARDS group (n=12).


RESULTS
In the BALF, only HSP 47 was significantly increased in ARDS group than non-ARDS group. In plasma, GRP 94, HSP 90, HSP 60, HSP 47, GPx-3, and IL-8 were increased significantly in ARDS group. In short, most of the AIM in BALF or plasma were not significantly different in ARDS group as compared with non-ARDS group. Ninety-day mortality was significantly related to HSP90, HSP 60 and GPx-3 in plasma but not in BALF.


CONCLUSIONS
Alteration of AIM levels in both BALF or plasma of ARDS group was not remarkable compared with the non-ARDS group. Our result suggests the need to reconsider ARDS pathophysiology and therapeutic strategy.
Division of Respiratory and Critical Care Medicine, Department of Internal Medicine, Korea University College of Medicine, Republic of Korea. pulsy@korea.ac.kr
Eun Joo
EJ
Lee
Ji-Youn
JY
Lim
Sang Yeub
SY
Lee
Seung Hyeon
SH
Lee
Kwang Ho
KH
In
Se Hwa
SH
Yoo
Donggeun
D
Sul
Sanghoon
S
Park
D000328:Adult; D000368:Aged; D000975:Antioxidants / Q000378:metabolism*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D016022:Case-Control Studies; D016207:Cytokines / Q000097:blood* / Q000378:metabolism; D005260:Female; D006360:Heat-Shock Proteins / Q000097:blood* / Q000378:metabolism; D006801:Humans; D018836:Inflammation Mediators / Q000097:blood / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D012128:Respiratory Distress Syndrome / Q000378:metabolism* / Q000401:mortality / Q000473:pathology
D000975:Antioxidants; D016207:Cytokines; D006360:Heat-Shock Proteins; D018836:Inflammation Mediators