Inactivation of recombinant bacteriophage lambda by use of chemical agents and UV radiation.
Several approaches for the inactivation of bacteriophage lambda, including UV germicidal irradiation (UVGI) and the chemical agents Virkon-S, Chloros, Decon-90, and sodium hydroxide (NaOH), were compared. Virkon, NaOH, and UVGI caused a ≥7-log(10) reduction in phage titers. This study successfully describes several methods with potential for bacteriophage inactivation in industrial settings.
BigDNA Limited, Roslin, Midlothian, Scotland, United Kingdom.
Ewan M
EM
Clark
Harry
H
Wright
Kelly-Anne
KA
Lennon
Vicki A
VA
Craik
Jason R
JR
Clark
John B
JB
March
D000998:Antiviral Agents / Q000378:metabolism*; D010582:Bacteriophage lambda / Q000187:drug effects / Q000235:genetics / Q000502:physiology* / Q000528:radiation effects; D050296:Microbial Viability / Q000187:drug effects* / Q000528:radiation effects*; D010545:Peroxides; D017670:Sodium Compounds; D013464:Sulfuric Acids; D014466:Ultraviolet Rays; D019562:Viral Load; D038301:Virus Inactivation
D000998:Antiviral Agents; D010545:Peroxides; D017670:Sodium Compounds; D013464:Sulfuric Acids; C524957:sodium hydride; C071517:monoperoxysulfate