Identification of a pheA gene associated with Streptococcus mitis by using suppression subtractive hybridization.
We performed suppression subtractive hybridization to identify genomic differences between Streptococcus mitis and Streptococcus pneumoniae. Based on the pheA gene, a primer set specific to S. mitis detection was found in 18 out of 103 S. mitis-specific clones. Our findings would be useful for discrimination of S. mitis from other closely related cocci in the oral environment.
Department of Microbiology, Chung-Ang University College of Medicine, Seoul, Republic of Korea.
Hee Kuk
HK
Park
Hien Thanh
HT
Dang
Soon Chul
SC
Myung
Wonyong
W
Kim
D017931:DNA Primers / Q000235:genetics; D005827:Genetics, Microbial / Q000379:methods; D009693:Nucleic Acid Hybridization / Q000379:methods; D011302:Prephenate Dehydratase / Q000235:genetics*; D034361:Streptococcus mitis / Q000145:classification / Q000201:enzymology* / Q000235:genetics*; D013296:Streptococcus pneumoniae / Q000145:classification / Q000201:enzymology / Q000235:genetics
D017931:DNA Primers; D011302:Prephenate Dehydratase