SapB and the rodlins are required for development of Streptomyces coelicolor in high osmolarity media.
Streptomyces coelicolor produces spore-forming aerial hyphae after a period of vegetative growth. These aerial structures are decorated with a hydrophobic coating of rodlets consisting of chaplins and rodlins. Here, we show that rodlins and the surface-active peptide SapB are essential for development during growth in a medium with high osmolarity. To this end, both vegetative and aerial hyphae secrete SapB, whereas rodlins are only secreted by the spore-forming aerial hyphae.
Department of Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands.
Wouter
W
de Jong
Erik
E
Vijgenboom
Lubbert
L
Dijkhuizen
Han A B
HA
Wösten
Dennis
D
Claessen
D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D003470:Culture Media; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D057927:Hydrophobic and Hydrophilic Interactions; D025301:Hyphae / Q000737:chemistry / Q000378:metabolism; D009994:Osmolar Concentration; D009997:Osmotic Pressure; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013172:Spores, Fungal / Q000737:chemistry / Q000378:metabolism; D048372:Streptomyces coelicolor / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
D001426:Bacterial Proteins; D003470:Culture Media; D005656:Fungal Proteins; C055447:Streptomyces spore-associated proteins