An improved single-step lysis protocol to measure luciferase bioluminescence in Plasmodium falciparum.
This report describes the optimization and evaluation of a simple single-step lysis protocol to measure luciferase bioluminescence from genetically modified Plasmodium falciparum. This protocol utilizes a modified commercial buffer to improve speed of assay and consistency in the bioluminescence signal measured by reducing the manipulation steps required to release the cytoplasmic fraction. The utility of this improved assay protocol is demonstrated in typical assays that explore absolute and temporal gene expression activity.
Institute for Science and Technology in Medicine, Keele University, Staffordshire ST5 5BG, UK.
Sandra
S
Hasenkamp
Eleanor H
EH
Wong
Paul
P
Horrocks
D000818:Animals; D003593:Cytoplasm / Q000201:enzymology; D006801:Humans; D008156:Luciferases / Q000032:analysis*; D049449:Luminescence; D030781:Organisms, Genetically Modified; D010274:Parasitology / Q000379:methods*; D010963:Plasmodium falciparum / Q000201:enzymology*
D008156:Luciferases