The three dimensionality of cell membranes: lamellar to cubic membrane transition as investigated by electron microscopy.
Biological membranes are generally perceived as phospholipid bilayer structures that delineate in a lamellar form the cell surface and intracellular organelles. However, much more complex and highly convoluted membrane organizations are ubiquitously present in many cell types under certain types of stress, states of disease, or in the course of viral infections. Their occurrence under pathological conditions make such three-dimensionally (3D) folded and highly ordered membranes attractive biomarkers. They have also stimulated great biomedical interest in understanding the molecular basis of their formation. Currently, the analysis of such membrane arrangements, which include tubulo-reticular structures (TRS) or cubic membranes of various subtypes, is restricted to electron microscopic methods, including tomography. Preservation of membrane structures during sample preparation is the key to understand their true 3D nature. This chapter discusses methods for appropriate sample preparations to successfully examine and analyze well-preserved highly ordered membranes by electron microscopy. Processing methods and analysis conditions for green algae (Zygnema sp.) and amoeba (Chaos carolinense), mammalian cells in culture and primary tissue cells are described. We also discuss methods to identify cubic membranes by transmission electron microscopy (TEM) with the aid of a direct template matching method and by computer simulation. A 3D analysis of cubic cell membrane topology by electron tomography is described as well as scanning electron microscopy (SEM) to investigate surface contours of isolated mitochondria with cubic membrane arrangement.
Cubic Membrane Laboratory, Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
Ketpin
K
Chong
Yuru
Y
Deng
D000656:Amoeba / Q000648:ultrastructure; D000818:Animals; D001402:B-Lymphocytes / Q000648:ultrastructure; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D021961:Cell Membrane Structures / Q000378:metabolism / Q000648:ultrastructure*; D002478:Cells, Cultured; D000460:Chlorophyta / Q000648:ultrastructure; D003198:Computer Simulation; D055032:Electron Microscope Tomography; D016591:Histocytological Preparation Techniques; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008854:Microscopy, Electron / Q000379:methods; D008928:Mitochondria / Q000648:ultrastructure; D008954:Models, Biological