Identification of the fur-binding site in regulatory region of the vulnibactin-receptor gene in Vibrio vulnificus.
The Vibrio vulnificus vuuA gene, of which expression is repressed by a complex of iron and ferric uptake regulator (Fur), was characterized to localize the Fur-binding site in its upstream regulatory region. In silico analysis suggested the presence of two possible Fur-binding sites; one is a classical Fur-box and the other is a previously reported distinct Fur-binding site. Site-directed mutagenesis and DNase I protection assays revealed the binding site for the iron-Fur complex, which includes an extended inverted repeat containing a homologous sequence to the classical Fur-box.
Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
Hyun-Jung
HJ
Lee
Kyu-Ho
KH
Lee
D000577:Amides / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites; D018983:DNA Footprinting; D004252:DNA Mutational Analysis; D004269:DNA, Bacterial / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010080:Oxazoles / Q000378:metabolism; D011485:Protein Binding; D012045:Regulatory Sequences, Nucleic Acid; D012097:Repressor Proteins / Q000378:metabolism*; D041261:Vibrio vulnificus / Q000235:genetics*
D000577:Amides; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D008565:Membrane Proteins; D010080:Oxazoles; D012097:Repressor Proteins; C068477:ferric uptake regulating proteins, bacterial; C086667:vulnibactin