Simple analysis of naphthalene, fluorene, and anthracene in whole blood by gas chromatography/mass spectrometry after headspace-solid phase microextraction.
A simple and sensitive method for the simultaneous analysis of naphthalene, fluorene, and anthracene in whole blood was developed using headspace-solid-phase microextraction (SPME) and GC/MS. A 0.5 g whole blood sample, 5 microL naphthalene, fluorene, and anthracene (50 microg/mL) as spiked standards, and 0.5 mL sodium hydroxide were placed into a 12 mL vial and sealed rapidly. The vial was immediately heated to 70 degrees C in an aluminium block heater, the needle of the SPME device was inserted through the septum of the vial, and the extraction fiber was exposed to the headspace for 15 min. Afterwards, the compounds extracted by the fiber were desorbed simultaneously by exposing the fiber in the gas chromatograph injection port. No interferences were found, and the time for analysis was about 30 min for one sample. This method was applied to a suicide case in which the victim ingested naphthalene, fluorene, and anthracene.
Pamukkale University, Faculty of Arts and Sciences, Department of Biochemistry, 20017 Denizli, Turkey. hardagakdogan@gmail.com
Hatice Ardag
HA
Akdoğan
D000873:Anthracenes / Q000032:analysis*; D005449:Fluorenes / Q000097:blood*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D057230:Limit of Detection; D009281:Naphthalenes / Q000097:blood*; D015203:Reproducibility of Results; D052617:Solid Phase Microextraction / Q000379:methods*; D013405:Suicide
D000873:Anthracenes; D005449:Fluorenes; D009281:Naphthalenes; C031721:naphthalene; C041509:fluorene; C034020:anthracene