Immunolocalization of β- and δ-giardin within the ventral disk in trophozoites of Giardia duodenalis using multiplex laser scanning confocal microscopy.
Immunolocalization of β- and δ-giardin in Giardia duodenalis trophozoites revealed that both giardins are strictly associated with the ventral disk (VD). Optical sectioning of the immunolabeled VD, together with quantitative colocalization of δ- and β-giardin immunoreactivity, demonstrated that δ-giardin is primarily localized to the ventral side, and β-giardin is localized to the dorsal side of the VD.
United States Department of Agriculture, Agricultural Research Service (USDA-ARS), Beltsville Agricultural Research Center (BARC), Environmental Microbial & Food Safety Laboratory, 10300 Baltimore Avenue, Bldg 173, BARC-East, Beltsville, MD 20705, USA. dumitru.macarisin@ars.usda.gov
Dumitru
D
Macarisin
Celia
C
O'Brien
Ronald
R
Fayer
Gary
G
Bauchan
Mark
M
Jenkins
D016829:Giardia lamblia / Q000737:chemistry*; D018613:Microscopy, Confocal / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D015800:Protozoan Proteins / Q000032:analysis*; D053058:Trophozoites / Q000737:chemistry
D015800:Protozoan Proteins