The effects of model polysiloxane and fouling-release coatings on embryonic development of a sea urchin (Arbacia punctulata) and a fish (Oryzias latipes).
In recent decades attention has focused on the development of non-toxic fouling-release coatings based on silicone polymers as an alternative to toxic antifouling coatings. As fouling-release coatings gain market share, they will contribute to environmental contamination by silicones. We report effects of eight model polysiloxane and three commercial foul-release coatings on embryonic development of sea urchins and fish, Japanese medaka. We used model coatings because they have known composition and commercially available components and molecules leaching from these coatings have been partially characterized. The commercial fouling-release coatings are purported to be non-toxic and components are proprietary. Our goal was to expose embryos of well studied model animals to the coatings to determine if the complex mixtures leaching from the coatings impact development. Urchins were chosen because development is rapid and embryos can enter the non-slip layer over surfaces. Medaka was chosen because the female deposits the sticky eggs onto the anal fin and then scrapes them off onto surfaces. Embryos were confined in water over coatings in 24 well plates. Fresh model coatings had no effect on urchin development while commercial fouling-release coatings inhibited development. Fish embryos had delayed hatching, increased mortality of hatchlings and dramatically decreased ability of hatchlings to inflate the swim bladder and reduced hatching success on all coatings. After one-month immersion of coatings in running seawater to simulate initial application in the marine environment, sea urchin embryos died when placed over model silicones. Effects of the commercial coatings were reduced but included retarded development. Effects on fish embryos over leached coating were reduced compared to those of fresh coating and included decreased hatching success, decreased hatchling survival and inability to inflate the swim bladder for commercial coatings. These findings suggest, similar to medical conclusions, compounds leaching from silicone coatings can impact development and the topic deserves study.
Duke University Marine Laboratory, Nicholas School of the Environment, Duke University, Beaufort, NC, United States.
Danqing
D
Feng
Daniel
D
Rittschof
Beatriz
B
Orihuela
Kevin Wing Hin
KW
Kwok
Shane
S
Stafslien
Bret
B
Chisholm
D000818:Animals; D047308:Arbacia / Q000196:embryology; D004202:Disinfectants / Q000633:toxicity; D004625:Embryo, Nonmammalian / Q000187:drug effects*; D047108:Embryonic Development / Q000187:drug effects; D009990:Oryzias / Q000196:embryology; D012623:Seawater / Q000737:chemistry; D012828:Silicones / Q000633:toxicity*; D012833:Siloxanes / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D004202:Disinfectants; D012828:Silicones; D012833:Siloxanes; D014874:Water Pollutants, Chemical