Associations of allergic sensitization and clinical phenotypes with innate immune response genes polymorphisms are modified by house dust mite allergen exposure.
BACKGROUND
Polymorphisms within innate immunity genes are associated with allergic phenotypes but results are variable. These associations were not analyzed with respect to allergen exposure. We investigated associations of TLR and CD14 polymorphisms with allergy phenotypes in the context of house dust mite (HDM) exposure.


METHODS
Children, aged 12-16 years (n=326), were recruited from downtown and rural locations and assessed by allergist. Skin prick tests, total and HDM-specific sIgE measurements were done. HDM allergen concentrations in dust were measured. Genetic polymorphisms were identified using restriction fragment length polymorphism (RFLP).


RESULTS
Allergic rhinitis, asthma and atopy were more prevalent in urban area. Although HDM allergen concentrations were higher in rural households, sIgE were present more frequently in urban children. In the whole population no association was found between HDM exposure and sensitization. In children with CD14/-159CC, CD14/-159TT and TLR9/2848GA genotypes increased exposure to HDM was associated with reduced incidence of allergic rhinitis. Significant associations of increased HDM exposure with reduced incidence of atopy were found for the whole population and subjects with CD14/-159CC, CD14/-1359GT, TLR4/896AA and TLR9/2848GA genotypes. Among children with CD14/-159CC and CD14/-1359GG significant positive correlation between HDM allergen concentrations in household and sensitization to HDM was observed. In contrast, protective effect of high HDM allergen exposure against specific sensitization was seen in subjects with TLR4/896 AG.


CONCLUSIONS
Development of specific sensitization and allergy may be associated with innate immune response genes polymorphisms and is modified by allergen exposure.
Department of Immunology, Rheumatology and Allergy, Medical University of Lodz, Poland.
Marcin
M
Kurowski
Barbara
B
Majkowska-Wojciechowska
Aleksandra
A
Wardzyńska
Marek L
ML
Kowalski