The TPX2 gene is a promising diagnostic and therapeutic target for cervical             cancer.
The target protein for Xklp2 (TPX2), a microtubule-associated protein, can             be used to evaluate more precisely the proliferative behavior of tumor cells.             The abnormal expression of TPX2 in various types of malignant tumors has been             reported, but less is known for cervical cancer. We studied the relationship between             TPX2 expression and the biological behavior of cervical cancer. Immunohistochemistry             and RT-PCR were used to detect the expression of TPX2 in cervical cancer tissues.             The inhibitory effect of TPX2-siRNA on the growth of HeLa human cervical carcinoma             cells was studied in vitro. TPX2 expression was found to be significantly higher             in cervical carcinoma compared to normal cervical tissues and CIN. The expression             of TPX2 in cervical cancer was correlated with histological grading, FIGO staging             and lymph node metastasis. TPX2 RNAi in HeLa cervical cancer cells caused S-phase             cell cycle arrest, induced apoptosis and inhibited cell proliferation and invasion.             In conclusion, TPX2 shows potential to be used as a new marker for cervical cancer             diagnosis and therapy.
Department of Obstetrics and Gynecology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, PR China.
Haiping
H
Chang
Jingzhi
J
Wang
Yuan
Y
Tian
Jie
J
Xu
Xiaojuan
X
Gou
Jianxin
J
Cheng
D000328:Adult; D000368:Aged; D017209:Apoptosis / Q000235:genetics; D002453:Cell Cycle / Q000235:genetics; D018797:Cell Cycle Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation / Q000235:genetics; D005260:Female; D020868:Gene Silencing; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism*; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D009687:Nuclear Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology
D018797:Cell Cycle Proteins; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; D034741:RNA, Small Interfering; C122167:TPX2 protein, human