Determination of the strength of adhesion between lipid vesicles.
A commonly used method to determine the strength of adhesion between adhering lipid vesicles is measuring their effective contact angle from experimental images. The aim of this paper is to estimate the interobserver variations in vesicles effective contact angle measurements and to propose a new method for estimating the strength of membrane vesicle adhesion. Theoretical model shows for the old and for the new measure a monotonic dependence on the strength of adhesion. Results obtained by both measuring techniques show statistically significant correlation and high interobserver reliability for both methods. Therefore the conventional method of measuring the effective contact angle gives qualitatively relevant results as the measure of the lipid vesicle adhesion. However, the new measuring technique provides a lower variation of the measured values than the conventional measures using the effective contact angle. Moreover, obtaining the adhesion angle can be automatized more easily than obtaining the effective contact angle.
Laboratory of Biomechanics, Faculty of Mechanical Engineering, CTU in Prague, Technická 4, 166 07 Prague 6, Czech Republic.
Tomáš
T
Mareš
Matej
M
Daniel
Aleš
A
Iglič
Veronika
V
Kralj-Iglič
Miha
M
Fošnarič
D000268:Adhesiveness; D001696:Biomechanical Phenomena; D022162:Cytoplasmic Vesicles / Q000737:chemistry* / Q000502:physiology; D008055:Lipids / Q000737:chemistry / Q000502:physiology; D008566:Membranes / Q000737:chemistry* / Q000502:physiology; D008858:Microscopy, Phase-Contrast; D015233:Models, Statistical; D015588:Observer Variation; D033081:Shear Strength / Q000502:physiology*; D013314:Stress, Mechanical
D008055:Lipids