Link between inflammation and aquaporin-5 distribution in submandibular gland in Sjögren's syndrome?
OBJECTIVE
To determine whether a link exists between inflammation and aquaporin-5 distribution in submandibular glands from three animal models for Sjögren's syndrome: IQI/JIC, r1ΔT/r2n and non-obese diabetic mice.


METHODS
Mice of different ages were used. Inflammatory infiltrates were quantified using the focus score. Acinar aquaporin-5 subcellular distribution was determined by immunohistochemistry and quantified using labelling indices.


RESULTS
Minor inflammatory infiltrates were present in r1f/r2n mice. Massive inflammatory infiltrates and acinar destruction were observed in 24-week-old non-obese diabetic mice, 10-and 13-month-old IQI/JIC mice and some r1ΔT/r2n mice. Aquaporin-5 immunoreactivity was primarily apical in submandibular glands from 8- and 24-week-old Balb/C mice, 8-week-old non-obese diabetic mice, 2-, 4- and 7-month-old IQI/JIC mice and r1f/r2n mice. In contrast, decreased apical aquaporin-5 labelling index with concomitant increased apical-basolateral, apical-cytoplasmic and/or apical-basolateral-cytoplasmic aquaporin-5 labelling indices was observed in 24-week-old non-obese diabetic, 10- and 13-month-old IQI/JIC and r1ΔT/r2n mice with a focus score≥1.


CONCLUSIONS
Altered aquaporin-5 distribution in submandibular acinar cells from IQI/JIC, non-obese diabetic and r1ΔT/r2n mice with a focus score≥1 appears to be concomitant to the presence of inflammatory infiltrates and acinar destruction.
Laboratory of Biological Chemistry and Nutrition, Université Libre de Bruxelles, Brussels, Belgium.
M S
MS
Soyfoo
A
A
Konno
N
N
Bolaky
J S
JS
Oak
D
D
Fruman
C
C
Nicaise
M
M
Takiguchi
C
C
Delporte
D061354:Acinar Cells / Q000473:pathology; D000367:Age Factors; D000818:Animals; D051402:Aquaporin 5 / Q000032:analysis*; D002462:Cell Membrane / Q000473:pathology; D003593:Cytoplasm / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016688:Mice, Inbred NOD; D008815:Mice, Inbred Strains; D008822:Mice, Transgenic; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics; D012793:Sialadenitis / Q000473:pathology*; D012859:Sjogren's Syndrome / Q000473:pathology*; D013347:Subcellular Fractions / Q000473:pathology; D013364:Submandibular Gland Diseases / Q000473:pathology*
C496038:Aqp5 protein, mouse; D051402:Aquaporin 5; D019869:Phosphatidylinositol 3-Kinases