On-line sample cleanup and enrichment chromatographic technique for the determination of ambroxol in human serum.
A sensitive and efficient on-line clean up and pre-concentration method has been developed using column-switching technique and protein-coated µ-Bondapak CN silica pre-column for quantification of ambroxol (AM) in human serum. The method is performed by direct injection of serum sample onto a protein-coated µ-Bondapak CN silica pre-column, where AM is pre-concentrated and retained, while proteins and very polar constituents are washed to waste using a phosphate buffer saline (pH 7.4). The retained analyte on the pre-column is directed onto a C(18) analytical column for separation, with a mobile phase consisting of a mixture of methanol and distilled deionized water (containing 1% triethylamine adjusted to pH 3.5 with ortho-phosphoric acid) in the ratio of 50:50 (v/v). Detection is performed at 254 nm. The calibration curve is linear over the concentration range of 12-120 ng/mL (r(2) = 0.9995). The recovery, selectivity, linearity, precision, and accuracy of the method are convenient for pharmacokinetic studies or routine assays.
Faculty of Pharmacy, Misr International University, Km 28 Ismailia Road, Cairo, Egypt. Emara_miu@yahoo.com
Samy
S
Emara
Maha
M
Kamal
Mohamed
M
Abdel Kawi
D000551:Ambroxol / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005100:Expectorants / Q000032:analysis*; D006801:Humans; D057230:Limit of Detection; D012015:Reference Standards
D005100:Expectorants; D000551:Ambroxol