Multifluorescence 2D gel imaging and image analysis.
Although image acquisition and analysis are crucial steps within the multifluorescence two-dimensional gel electrophoresis workflow, some basics are frequently not carried out with the necessary diligence. This chapter should help to prevent easily avoidable failures during imaging and image preparation for comparative protein analysis.
Institute of Clinical Biochemistry and Pathobiochemistry, German Diabetes Center, Düsseldorf, Germany.
Ingo
I
Vormbrock
Sonja
S
Hartwig
Stefan
S
Lehr
D007091:Image Processing, Computer-Assisted / Q000379:methods*; D012984:Software; D013050:Spectrometry, Fluorescence / Q000379:methods*; D057825:Two-Dimensional Difference Gel Electrophoresis / Q000379:methods*