Comparative high-throughput RNAi screening methodologies in C. elegans and mammalian cells.
The discovery of RNAi in Caenorhabditis elegans has generated a paradigm shift in how research is performed. Targeted gene knockdown using high throughput screening approaches is becoming a routine feature of the scientific landscape, and researchers can now evaluate the function of each gene in the genome in a relatively short period of time. This review compares and contrasts high throughput screening methodologies in C. elegans and mammalian cells and highlights the breadth of applications of this technology.
Victorian Centre for Functional Genomics, Peter MacCallum Cancer Centre, Australia. kaylene.simpson@petermac.org
Kaylene J
KJ
Simpson
Gregory M
GM
Davis
Peter R
PR
Boag
D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics; D019295:Computational Biology; D017930:Genes, Reporter; D023281:Genomics; D057166:High-Throughput Screening Assays; D006801:Humans; D023421:Models, Animal; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism
D034741:RNA, Small Interfering