Transcript profiling using ESTs from Paracoccidioides brasiliensis in models of infection.
Transcript profiling is an invaluable strategy to study differential gene expression. Here we describe a detailed protocol for applying a subtractive hybridization technique, representational difference analysis (RDA), as a molecular strategy for the identification of differentially expressed genes in studies of host-fungus interaction. Bioinformatics tools that can be used in the analysis of expressed sequence tags (ESTs) are also detailed.
Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, ICBII, Universidade Federal de Goiás, Campus II, Goiás, Brazil.
Alexandre Melo
AM
Bailão
Maristela
M
Pereira
Silvia Maria
SM
Salem-Izacc
Clayton Luiz
CL
Borges
Célia Maria de Almeida
CM
Soares
D056809:Alveolar Epithelial Cells / Q000166:cytology / Q000276:immunology / Q000382:microbiology; D000818:Animals; D019295:Computational Biology; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D054884:Host-Pathogen Interactions; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010228:Paracoccidioides / Q000235:genetics* / Q000276:immunology / Q000502:physiology; D010229:Paracoccidioidomycosis / Q000276:immunology / Q000382:microbiology