Comparative 2D DIGE analysis of the depleted serum proteome for biomarker discovery.
Serum is unarguably the most used diagnostic fluid. As it circulates throughout the body, leakage peptides/proteins from damaged and dying cells, host-response proteins including inflammatory mediators, and aberrant secretions from tumors and diseased tissues are released into serum, potentially providing a rich source of disease biomarkers. Here, a method for extending access to the serum proteome by removing highly abundant proteins prior to comparative two-dimensional difference gel electrophoresis (2D DIGE) and subsequent protein digestion for identification by mass spectrometry is described.
Adelaide Proteomics Centre, University of Adelaide, Adelaide, Australia.
Megan
M
Penno
Matthias
M
Ernst
Peter
P
Hoffmann
D053000:Analytic Sample Preparation Methods; D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000737:chemistry / Q000378:metabolism; D001798:Blood Proteins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D002232:Carbocyanines / Q000737:chemistry; D004396:Coloring Agents / Q000737:chemistry; D007091:Image Processing, Computer-Assisted; D051379:Mice; D059748:Proteolysis; D020543:Proteome / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D013194:Staining and Labeling; D057825:Two-Dimensional Difference Gel Electrophoresis / Q000379:methods*
D015415:Biomarkers; D001798:Blood Proteins; D002232:Carbocyanines; D004396:Coloring Agents; D020543:Proteome