Expression turnover profiling to monitor the antifungal activities of amphotericin B, voriconazole, and micafungin against Aspergillus fumigatus.
Eight highly expressed candidate genes were selected for mRNA profiling to monitor the transcriptome kinetics of Aspergillus fumigatus strains exposed to antifungal drugs as potential biomarkers of live cells to assess treatment efficacy. Mycelia were treated with fungicidal drugs amphotericin B and voriconazole, as well as the fungistatic drug micafungin. Transcription was monitored at 0, 4, 8, and 24 h posttreatment. The expression turnover profile provides a possible tool to assess antifungal therapy effects.
Public Health Research Institute Center, UMDNJ-New Jersey Medical School, Newark, New Jersey, USA..
Yanan
Y
Zhao
Padmaja
P
Paderu
Steven
S
Park
Aleksandra
A
Dukhan
Meredith
M
Senter
David S
DS
Perlin
D000666:Amphotericin B / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D001232:Aspergillus fumigatus / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D054714:Echinocandins / Q000494:pharmacology*; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D006801:Humans; D055666:Lipopeptides / Q000494:pharmacology*; D000077551:Micafungin; D011743:Pyrimidines / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis; D059467:Transcriptome / Q000187:drug effects; D016896:Treatment Outcome; D014230:Triazoles / Q000494:pharmacology*; D065819:Voriconazole
D000935:Antifungal Agents; D054714:Echinocandins; D005656:Fungal Proteins; D055666:Lipopeptides; D011743:Pyrimidines; D012333:RNA, Messenger; D014230:Triazoles; D000666:Amphotericin B; D065819:Voriconazole; D000077551:Micafungin