Mammalian-specific OR37 receptors are differentially activated by distinct odorous fatty aldehydes.
The capacity of the mammalian olfactory system to detect an enormous collection of different chemical compounds is based on a large repertoire of odorant receptors (ORs). A small group of these ORs, the OR37 family, is unique due to a variety of special features. Members of this subfamily are exclusively found in mammals, they share a high degree of sequence homology and are highly conserved during evolution. It is still elusive which odorants may activate these atypical receptors. We have reasoned that compounds from skin, hairs, or skin glands might be potential candidates. We have exposed mice to such compounds and monitored activation of glomeruli through the expression of the activity marker c-fos in juxtaglomerular cells surrounding ventrally positioned glomeruli in the olfactory bulb (OB). Employing this methodology it was found that stimulation with long-chain alkanes elicits activation in the ventral part of the OB, however, none of the OR37 glomeruli. Analyses of long-chain hydrocarbon compounds with different functional groups revealed that long-chain aliphatic aldehydes elicited an activation of defined OR37 glomeruli, each of them responding preferentially to an aldehyde with different chain lengths. These results indicate that OR37 receptors may be tuned to distinct fatty aldehydes with a significant degree of ligand specificity.
Institute of Physiology, University of Hohenheim, Garbenstrasse 30, 70593 Stuttgart, Germany.
Verena
V
Bautze
Raphaela
R
Bär
Benjamin
B
Fissler
Michaela
M
Trapp
Dietmar
D
Schmidt
Uwe
U
Beifuss
Bernd
B
Bufe
Frank
F
Zufall
Heinz
H
Breer
Jörg
J
Strotmann
D000447:Aldehydes / Q000494:pharmacology*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D005227:Fatty Acids / Q000494:pharmacology*; D005786:Gene Expression Regulation; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D009812:Odorants; D009830:Olfactory Bulb / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D020033:Protein Isoforms / Q000819:agonists / Q000235:genetics / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism; D018035:Receptors, Odorant / Q000819:agonists* / Q000235:genetics / Q000378:metabolism; D012903:Smell / Q000187:drug effects* / Q000502:physiology
D000447:Aldehydes; D015415:Biomarkers; D005227:Fatty Acids; C452230:Olfr37 protein, mouse; D020033:Protein Isoforms; D016760:Proto-Oncogene Proteins c-fos; D018035:Receptors, Odorant; C001634:fatty aldehyde