Effect of matrigel on the osteogenic potential of canine adipose tissue-derived mesenchymal stem cells.
Adipose tissue-derived mesenchymal stem cells (Ad-MSCs) are a promising source of cells for bone tissue engineering. Matrigel is a basement membrane extract containing multiple extracellular components. This mixture may promote the osteogenic differentiation of MSCs and provide a more appropriate microenvironment for transplanted cells. Here, we investigated the effect of Matrigel on the osteogenic potential of Ad-MSCs. Canine Ad-MSCs were cultured in 2D and 3D matrices and implanted into subcutaneous pouches of dogs either with or without Matrigel. Culture mineralization, cell adhesion efficiency, cell proliferation, osteoid matrix production and alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase activities were quantified and compared. Ad-MSCs grown in 2D cultures with Matrigel showed higher levels of calcium deposition and ALP activity than those grown in the absence of Matrigel under osteogenic conditions. In 3D cultures, the cells cultivated with Matrigel showed greater attachment, proliferation and osteogenic differentiation than those grown without Matrigel. In vivo, Ad-MSCs implanted with Matrigel showed higher osteogenic potential than those without Matrigel. In conclusion, these data suggest that the use of Matrigel can increase the osteogenic potential of canine Ad-MSCs.
Department of Veterinary Surgery, College of Veterinary Medicine, Seoul National University, Daehak-dong, Gwanak-gu, Seoul 151-742, Korea.
Byung-Jae
BJ
Kang
Hak-Hyun
HH
Ryu
Sung-Su
SS
Park
Yongsun
Y
Kim
Heung-Myong
HM
Woo
Wan Hee
WH
Kim
Oh-Kyeong
OK
Kweon
D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002130:Calcium Phosphates; D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques / Q000662:veterinary; D002454:Cell Differentiation / Q000187:drug effects* / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects; D003094:Collagen / Q000008:administration & dosage / Q000494:pharmacology*; D004285:Dogs; D004338:Drug Combinations; D007797:Laminin / Q000008:administration & dosage / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000502:physiology; D010012:Osteogenesis / Q000187:drug effects* / Q000502:physiology; D011509:Proteoglycans / Q000008:administration & dosage / Q000494:pharmacology*; D018709:Statistics, Nonparametric; D013778:Tetrazolium Salts; D013844:Thiazoles; D023822:Tissue Engineering / Q000379:methods*
D002130:Calcium Phosphates; D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; D013778:Tetrazolium Salts; D013844:Thiazoles; C485817:beta-tricalcium phosphate; C059485:matrigel; D003094:Collagen; C022616:thiazolyl blue