Highly efficient synthesis of sucrose monolaurate by alkaline protease Protex 6L.
An alkaline protease from Bacillus licheniformis, Protex 6L, was used for synthesis of sucrose monolaurate from sucrose and vinyl laurate in a tert-amyl alcohol/DMSO/water solvent mixture. Introducing sucrose as powder after mixing vinyl laurate with solvent mixture resulted in a higher reaction rate than when sucrose was added as a solution in DMSO. Response surface methodology (RSM) was applied to optimize the major reaction variables, water content, temperature and pH of the lyophilized enzyme. The optimal conditions derived from RSM (3.4% water content, 43 °C and pH of 10.1) provided a high initial reaction rate of 8.66 ± 0.3 mg/ml/h which agreed with the predicted value of 8.70 mg/ml/h. With 1.5 mg-enzyme/ml, 98.0% of the added sucrose was region-selectively converted to 1'-O-lauroylsucrose after 9h. Under the optimized conditions, Protex 6L exhibited a higher productivity for sucrose ester synthesis than Novozym 435 and three other commonly used enzymes.
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
Xinran
X
Wang
Shida
S
Miao
Ping
P
Wang
Songping
S
Zhang
D000704:Analysis of Variance; D001407:Bacillus / Q000201:enzymology*; D001426:Bacterial Proteins / Q000378:metabolism*; D055162:Biocatalysis; D001709:Biotechnology / Q000379:methods*; D010450:Endopeptidases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008954:Models, Biological; D012995:Solubility; D012997:Solvents; D013379:Substrate Specificity; D013395:Sucrose / Q000031:analogs & derivatives* / Q000378:metabolism; D013997:Time Factors
D001426:Bacterial Proteins; D012997:Solvents; C018152:sucrose monolaurate; D013395:Sucrose; D010450:Endopeptidases; C087822:alkaline protease