Characterization of hydrogen sulfide and its synthases, cystathionine β-synthase and cystathionine γ-lyase, in human prostatic tissue and cells.
OBJECTIVE
To investigate hydrogen sulfide and its synthases, cystathionine β-synthase (CBS) and cystathionine γ-lyase (CSE), in human prostatic tissue and cells.


METHODS
CBS and CSE in human prostatic tissue and cells were located using immunostaining. Western blot and a sulfur-sensitive electrode were used to evaluate the expression levels and catalytic activity of CBS and CSE. We analyzed the association between dihydrotestosterone-added or hormone-reduced medium-induced CBS/CSE protein levels with androgen receptor levels in prostate cancer lines. All experiments were repeated ≥3 times.


RESULTS
Endogenous hydrogen sulfide and its synthases existed in various areas of human prostatic tissue and cells. Cell activity and CBS/CSE protein levels were greatest in the androgen-dependent prostate cancer cell LNCaP among all cells and downregulated by dihydrotestosterone.


CONCLUSIONS
Hydrogen sulfide and its synthases in human prostatic tissue and cells were modulated by dihydrotestosterone, which could suggest a potential therapy for prostatic disease.
Department of Urology, Peking University First Hospital, and Institute of Urology, Peking University, Beijing, China.
Hui
H
Guo
Jun-Wei
JW
Gai
Ying
Y
Wang
Hong-Fang
HF
Jin
Jun-Bao
JB
Du
Jie
J
Jin
D000230:Adenocarcinoma / Q000737:chemistry / Q000201:enzymology / Q000473:pathology; D000728:Androgens; D002460:Cell Line / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology; D045744:Cell Line, Tumor / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology; D003470:Culture Media / Q000494:pharmacology; D003541:Cystathionine beta-Synthase / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D003542:Cystathionine gamma-Lyase / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D013196:Dihydrotestosterone / Q000494:pharmacology; D015536:Down-Regulation / Q000187:drug effects; D004790:Enzyme Induction / Q000187:drug effects; D004847:Epithelial Cells / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D006862:Hydrogen Sulfide / Q000032:analysis*; D008297:Male; D009363:Neoplasm Proteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D009376:Neoplasms, Hormone-Dependent / Q000737:chemistry / Q000201:enzymology / Q000473:pathology; D011467:Prostate / Q000737:chemistry* / Q000166:cytology / Q000201:enzymology; D011471:Prostatic Neoplasms / Q000737:chemistry / Q000201:enzymology / Q000473:pathology; D011944:Receptors, Androgen / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D017154:Stromal Cells / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology
D000728:Androgens; D003470:Culture Media; D009363:Neoplasm Proteins; D011944:Receptors, Androgen; D013196:Dihydrotestosterone; D003541:Cystathionine beta-Synthase; D003542:Cystathionine gamma-Lyase; D006862:Hydrogen Sulfide