Identification and characterization of lipopolysaccharide binding protein (LBP) as an estrogen receptor α specific serum biomarker.
Estrogen Receptor α (ERα) and Estrogen Receptor β (ERβ) are steroid nuclear receptors that transduce estrogen signaling to control diverse physiological processes linked to reproduction, bone remodeling, behavior, immune response and endocrine-related diseases. In order to differentiate between ERα and ERβ mediated effects in vivo, ER subtype selective biomarkers are essential. We utilized ERα knockout (AERKO) and ERβ knockout (BERKO) mouse liver RNA and genome wide profiling to identify novel ERα selective serum biomarker candidates. Results from the gene array experiments were validated using real-time RT-PCR and subsequent ELISA's to demonstrate changes in serum proteins. Here we present data that Lipopolysacharide Binding Protein (LBP) is a novel liver-derived ERα selective biomarker that can be measured in serum.
Department of Molecular Endocrinology, NJ, USA. michael_chisamore@merck.com
Michael J
MJ
Chisamore
Kwok-Lam Karen
KL
Hong
Chun
C
Cheng
Stephen E
SE
Alves
Susan P
SP
Rohrer
Hilary A
HA
Wilkinson
D000209:Acute-Phase Proteins; D000818:Animals; D015415:Biomarkers / Q000097:blood*; D001798:Blood Proteins / Q000032:analysis*; D002352:Carrier Proteins / Q000097:blood*; D004958:Estradiol / Q000008:administration & dosage; D047628:Estrogen Receptor alpha / Q000172:deficiency / Q000235:genetics*; D047629:Estrogen Receptor beta / Q000172:deficiency / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000097:blood*; D051379:Mice; D018345:Mice, Knockout; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis*; D051381:Rats; D014599:Uterus / Q000187:drug effects / Q000378:metabolism; D000099231:Lipopolysaccharide-Binding Protein
D000209:Acute-Phase Proteins; D015415:Biomarkers; D001798:Blood Proteins; D002352:Carrier Proteins; D004958:Estradiol; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D000099231:Lipopolysaccharide-Binding Protein