Parasite-specific aptamers as biosynthetic reagents and potential pharmaceuticals.
Aptamers are short, synthetic nucleic acid molecules. They are generated by a Darwinian-type in vitro evolution method known as 'systematic evolution of ligands by exponential enrichment' (SELEX). SELEX represents an experimental platform to identify rare ligands with predetermined functionality from combinatorial nucleic acid libraries. Since its discovery about 20 years ago the method has been instrumental in identifying a large number of aptamers that recognize targets of very different chemistry and molecular complexity. Although aptamers have been converted into sophisticated biomolecular tools for a diverse set of technologies, only a limited number of aptamers have been selected as binding reagents for parasites or parasite-derived molecules. Here the published examples of aptamers that target Leishmania-, Trypanosoma- and Plasmodia-specific molecules are reviewed.
Genetics, Darmstadt University of Technology, Schnittspahnstrasse 10, 64287 Darmstadt, Germany. goringer@hrzpub.tu-darmstadt.de
H Ulrich
HU
Göringer
D000818:Animals; D000978:Antiparkinson Agents / Q000494:pharmacology*; D052157:Aptamers, Nucleotide / Q000378:metabolism*; D019020:Directed Molecular Evolution / Q000379:methods; D055808:Drug Discovery; D010271:Parasites / Q000378:metabolism*
D000978:Antiparkinson Agents; D052157:Aptamers, Nucleotide