Dysgonomonas oryzarvi sp. nov., isolated from a microbial fuel cell.
A Gram-stain-negative, non-motile and coccoid- to short-rod-shaped bacterium, designated strain Dy73(T), was isolated from a microbial fuel cell that had been inoculated with rice paddy field soil and fed starch, peptone and fish extract as fuels. On the basis of 16S rRNA gene sequence phylogeny, strain Dy73(T) was affiliated with the genus Dysgonomonas in the phylum Bacteroidetes, and most closely related to Dysgonomonas mossii CCUG 43457(T) with a 16S rRNA gene sequence similarity value of 99.7 %. However, the DNA-DNA relatedness value between strain Dy73(T) and Dysgonomonas mossii CCUG 43457(T) was 34.8%. In addition, strain Dy73(T) was found to be different from other recognized species of the genus Dysgonomonas in taxonomically important traits, including habitat, DNA G+C content, bile resistance and fatty-acid composition. Based on these characteristics, strain Dy73(T) represents a novel species of the genus Dysgonomonas for which the name Dysgonomonas oryzarvi sp. nov. is proposed. The type strain is Dy73(T) ( = JCM 16859(T) = KCTC 5936(T)).
Institute for Materials Research, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai, Miyagi 980-8577, Japan.|Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
Yumiko
Y
Kodama
Department of Biomolecular Engineering, Tohoku University, 6-6-11 Aramaki Aza Aoba, Aoba-ku, Sendai, Miyagi 980-8579, Japan.
Takefumi
T
Shimoyama
School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Horinouchi, Hachioji, Tokyo 192-0392, Japan.|Hashimoto Light Energy Conversion Project, Exploratory Research for Advanced Technology, Japan Science and Technology Agency (JST), 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.|Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
Kazuya
K
Watanabe
D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D001674:Bioelectric Energy Sources / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S