Hypotensive effect of Oxacom® containing a dinitrosyl iron complex with glutathione: animal studies and clinical trials on healthy volunteers.
A comparative study of hypotensive effects of binuclear forms of dinitrosyl iron complexes (DNICs) with glutathione, S-nitrosoglutathione (GS-NO) and sodium nitrite (NaNO(2)) on rats has been carried out. The latter appeared to be the least efficient, viz., mean arterial pressure (MAP) decreased by 10 and 30 mmHg at 25 and 100 μmoles/kg of NaNO(2). In contrast, DNIC and GS-NO produced an appreciable hypotensive effect when used at much lower concentrations. GS-NO reduced MAP to the same extent, viz., to 90 mmHg, on a hundredfold dose scale (from 0.4 up to 50 μmoles/kg) with subsequent restoration of MAP within the next 6-15 min. A similar effect was observed for DNIC except that the amplitude of the MAP drop was lower and the duration of hypotension was essentially greater. DNIC with glutathione were selected as a basic material for pilot-scale production of a hypotensive drug (commercial name Oxacom®). Preliminary pharmacological testing of Oxacom did not establish any adverse or deleterious side effects. Clinical trials of Oxacom® were performed on 14 healthy male volunteers in whom single intravenous infusion of the drug (5mg/kg or 0.2 μmoles/kg of DNIC, respectively) evoked a characteristic response manifested as a 3-4 min drop by 24-27 mmHg of both diastolic and systolic AP with its subsequent slow restoration within the next 8-10h. The heart rate was quickly normalized after an initial increase. Cardiac output was unchanged despite reduced cardiac filling. A comprehensive analysis of clinical and biochemical data failed to establish any significant pathological changes in these parameters. The data obtained suggest that Oxacom® can be recommended for the second phase of clinical trials.
Russian Cardiological Research and Productive Complex, Russian Ministry of Health, Moscow, Russia.
Evgeny I
EI
Chazov
Oleg V
OV
Rodnenkov
Andrey V
AV
Zorin
Vladimir L
VL
Lakomkin
Vladimir V
VV
Gramovich
Oleg N
ON
Vyborov
Anatoly G
AG
Dragnev
Capital A Cyrilliclexander А
CA
Timoshin
Lyudmila I
LI
Buryachkovskaya
Alexander A
AA
Abramov
Valery P
VP
Massenko
Evgeny V
EV
Arzamastsev
Valery I
VI
Kapelko
Anatoly F
AF
Vanin
D000328:Adult; D000818:Animals; D000959:Antihypertensive Agents / Q000009:adverse effects / Q000097:blood / Q000494:pharmacology* / Q000633:toxicity*; D001794:Blood Pressure / Q000187:drug effects*; D002302:Cardiac Output / Q000187:drug effects; D004622:Embryo, Mammalian / Q000187:drug effects; D005260:Female; D005296:Ferrous Compounds / Q000009:adverse effects / Q000097:blood / Q000494:pharmacology* / Q000633:toxicity; D005978:Glutathione / Q000009:adverse effects / Q000031:analogs & derivatives* / Q000097:blood / Q000494:pharmacology / Q000633:toxicity; D006487:Hemostasis / Q000187:drug effects; D006728:Hormones / Q000097:blood; D006801:Humans; D008297:Male; D010973:Platelet Adhesiveness / Q000187:drug effects; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D018675:Toxicity Tests
D000959:Antihypertensive Agents; D005296:Ferrous Compounds; D006728:Hormones; D005978:Glutathione