Padlock probes and rolling circle amplification for detection of repeats and single-copy genes in the single-cell comet assay.
Padlock probes and rolling circle amplification are techniques which can be used for detection of DNA sequences in situ with high specificity and high signal to noise. The single-cell gel electrophoresis assay is used to measure DNA damage and repair in cells. Here, we describe how padlock probes and rolling circle amplification can be used to detect DNA sequences within comet preparations.
Department of Genetics and Pathology, Uppsala University, Uppsala, Sweden.
Sara
S
Henriksson
Mats
M
Nilsson
D000818:Animals; D001483:Base Sequence; D020552:Comet Assay / Q000379:methods*; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D004249:DNA Damage; D018628:Gene Dosage; D006801:Humans; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes / Q000737:chemistry / Q000235:genetics*; D059010:Single-Cell Analysis / Q000379:methods*
D015345:Oligonucleotide Probes; D004247:DNA