2D DIGE analysis of protein extracts from muscle tissue.
2D DIGE, two-dimensional difference gel electrophoresis, is a technology used to study the protein expression on two-dimensional gels. Protein samples are labeled with different color fluorescent dyes designed not to affect the relative migration of proteins during electrophoresis. Here, we describe the practical procedures necessary to perform a 2D DIGE experiment for a muscle tissue protein extract followed by CyDye DIGE fluors minimal labeling and the analysis of 2D DIGE gels for the assessment of quantitative differences.
Dipartimento di Scienze e Tecnologie Biomediche, Università degli Studi di Milano, Segrate, Italy. cecilia.gelfi@unimi.it
Cecilia
C
Gelfi
Sara
S
De Palma
D006801:Humans; D007091:Image Processing, Computer-Assisted; D007525:Isoelectric Focusing; D009124:Muscle Proteins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*; D018482:Muscle, Skeletal / Q000166:cytology*; D013194:Staining and Labeling; D057825:Two-Dimensional Difference Gel Electrophoresis / Q000379:methods*
D009124:Muscle Proteins