Semi-continuous production of 2-keto-gluconic acid by Pseudomonas fluorescens AR4 from rice starch hydrolysate.
2-Keto-gluconic acid (2KGA) was produced in a semi-continuous process using Pseudomonas fluorescens AR4 and rice starch hydrolysate (RSH). The bacterium was cultured in medium with an initial glucose concentration of 170g/L supplied as RSH. Once the glucose level had dropped to 20g/L, 60% of the culture volume was replaced with fresh medium containing 190g/L of glucose in the form of RSH. After an additional two cycles of growth and media replacement, a total of 476.88g/L of glucose was consumed and 444.96g/L of 2KGA was produced. A total productivity of 6.74g/L and a yield of 0.93g/g were obtained. These findings suggest that P. fluorescens AR4 is suitable for the production of commercially acceptable levels of 2KGA in semi-continuous culture.
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
Wen-Jing
WJ
Sun
Yan-Zheng
YZ
Zhou
Qiang
Q
Zhou
Feng-Jie
FJ
Cui
Shi-Lian
SL
Yu
Lei
L
Sun
D003470:Culture Media; D005285:Fermentation; D005942:Gluconates / Q000378:metabolism*; D006868:Hydrolysis; D012275:Oryza / Q000378:metabolism*; D011551:Pseudomonas fluorescens / Q000378:metabolism*
D003470:Culture Media; D005942:Gluconates; C030691:gluconic acid