Three new cycloartenol glycosides from the roots of Cimicifuga simplex.
Three new cycloartenol triterpene saponins, 3 β,16 α-dihydroxy-12-acetoxy-16,22-cyclo-23-ketone-24 R,25-epoxy-cycloartane-7-ene 3- O- β-D-galactopyranoside ( 1), 24- O-hydroxy-7,8-didehydrohydroshengmanol 3- O- β-D-galactopyranoside ( 2), and 24-epi-24- O-hydroxy-7,8-didehydrohydroshengmanol 3- O- β-D-galactopyranoside ( 3), were isolated from the ethyl acetate soluble fraction of Cimicifuga simplex. Their structures were established by detailed spectroscopic analysis, including extensive 2D-NMR data. This is the first time that a 16,22-cyclo type glycosidesaponin from aCimicifuga species was reported. The immunosuppressive activities of the new compounds were evaluated by a ConA-stimulated T splenocyte proliferation assay in vitro.
Key Laboratory of the Ministry of Education, Department of Pharmacognosy, Heilongjiang University of Chinese Medicine, Harbin, China.
HaiXue
H
Kuang
Yang
Y
Su
QiuHong
Q
Wang
Lun
L
Wu
BingYou
B
Yang
ZhiBin
Z
Wang
YongGang
Y
Xia
D045744:Cell Line, Tumor; D049109:Cell Proliferation; D031885:Cimicifuga / Q000737:chemistry*; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D007166:Immunosuppressive Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010840:Phytosterols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification; D018517:Plant Roots / Q000737:chemistry; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects*; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
C576428:24-O-hydroxy-7,8-didehydrohydroshengmanol-3-O-beta-D-galactopyranoside; C576429:24-epi-24-O-hydroxy-7,8-didehydrohydroshengmanol-3-D-beta-D-galactopyranoside; C576427:3beta,16alpha-dihydroxy-12-acetoxy-16,22-cyclo-23-ketone-24 R,25-epoxy-cycloartane-7-ene 3-O- beta-D-galactopyranoside; D006027:Glycosides; D007166:Immunosuppressive Agents; D010840:Phytosterols; D010936:Plant Extracts; D014315:Triterpenes; C100089:cycloartenol