Intra-age, intercenter, and intercycle differences in chromosome abnormalities in oocytes.
OBJECTIVE
To determine the extent of intra-age and intercycle variations in the frequency of first polar body aneuploidy in two consecutive cycles of oocyte retrieval undertaken by the same patient within 1 year.


METHODS
Retrospective study.


METHODS
Fertility centers.


METHODS
Infertile couples undergoing IVF.


METHODS
Patients underwent two consecutive cycles of preimplantation genetic screening through first polar body biopsy within 1 year.


METHODS
Meiosis I aneuploidy.


RESULTS
A total of 226 patients underwent 452 cycles of preimplantation genetic screening. Differences within age groups were wide, with 0-100% of oocytes being chromosomally normal in all age groups. Euploidy rates between centers were significantly different (48% vs. 25%). Intercycle differences for the same patient were also wide (0-100%), but with 68.5% of patients having less than ±2 euploid eggs of difference between cycles.


CONCLUSIONS
Although euploidy rate decreased on average with advancing maternal age, the high intra-age and intercenter variation in oocyte chromosome abnormalities emphasize the difficulty in estimating how many euploid oocytes a specific woman will have. This may have repercussions for fertility preservation where a defined number of eggs are currently frozen just based on maternal age.
Reprogenetics, Livingston, New Jersey, USA. munne@reprogenetics.com
Santiago
S
Munné
Karsten R
KR
Held
Cristina M
CM
Magli
Baris
B
Ata
Dagan
D
Wells
Elpida
E
Fragouli
Vera
V
Baukloh
Robert
R
Fischer
Luca
L
Gianaroli
D000328:Adult; D000367:Age Factors; D000782:Aneuploidy; D001706:Biopsy; D002869:Chromosome Aberrations; D002877:Chromosomes, Human; D015925:Cryopreservation; D005260:Female; D005300:Fertility Agents, Female / Q000009:adverse effects*; D005820:Genetic Testing; D005858:Germany; D006268:Health Facilities; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007246:Infertility / Q000503:physiopathology / Q000628:therapy*; D007558:Italy; D016014:Linear Models; D008540:Meiosis; D008875:Middle Aged; D009865:Oocytes / Q000187:drug effects* / Q000473:pathology; D010060:Ovulation / Q000187:drug effects*; D010062:Ovulation Induction / Q000009:adverse effects*; D059705:Polar Bodies / Q000187:drug effects / Q000473:pathology; D019836:Preimplantation Diagnosis / Q000379:methods; D012189:Retrospective Studies; D018570:Risk Assessment; D012307:Risk Factors; D013997:Time Factors
D005300:Fertility Agents, Female