Temperature-dependent self-splicing group I introns in the flagellin genes of the thermophilic Bacillus species.
A previous report described the presence of a self-splicing group I intron in a flagellin gene from a thermophilic Bacillus species. Here, we present evidence that the splicing reaction of the flagellin introns is dependent on temperature. Furthermore, a complementation analysis using a Bacillus subtilis flagellin-deficient mutant indicated that the intron-containing flagellin gene significantly restored the motility of the mutant at higher temperatures.
Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University, Tokyo, Japan.
Jumpei
J
Hayakawa
Morio
M
Ishizuka
D001407:Bacillus / Q000235:genetics*; D005408:Flagellin / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics; D005816:Genetic Complementation Test; D007438:Introns / Q000235:genetics*; D012326:RNA Splicing; D013696:Temperature
D005408:Flagellin