Loss of heterozygosity at 6p21 and HLA class I expression in esophageal squamous cell carcinomas in China.
OBJECTIVE
The loss or downregulation of human leukocyte antigen ( HLA-I) has been proposed to contribute to immune evasion by cancer cells. Since the human leukocyte antigen (HLA-I) complex is located at 6p21.3, loss of heterozygosity of this region may alter HLA class I tumor phenotypes. The aim of this study was to analysis loss of heterozygosity (LOH) of chromosome 6p in ESCC samples and correlate this with HLA class I expression.


METHODS
A total of 87 formalin-fixed, paraffin-embeded and frozen-fresh of ECSS lesions were collected. HLA-Iand antigen-processing machinery component expression was investigated by immunohistochemistry with anti-HLA class I monoclonal antibody and a panel of 49 ESCCs with downregulated HLA class I expression were selected for LOH studies using 3 microsatellite markers located at 6p21.3 (D6S105,D6S265,D6S273).


RESULTS
HLA-Iantigen,TAP1 and LMP were lost or down-regulated in 57.5, 29.8 and 47.0% of the ESCC lesions, respectively. In 23/49(46.9%) of the ESCCs, allelic loss for at least one locus at 6p21.3 was found.


CONCLUSIONS
Our data show that downregulation of HLA class I expression is correlated with loss of heterozygosity regions at 6p21.3 in ESCC.
Thoracic Surgery, Second Hospital of Shandong University, Jinan, China.
Xiaogang
X
Zhao
Qifeng
Q
Sun
Hui
H
Tian
Bo
B
Cong
Xingtao
X
Jiang
Chuanliang
C
Peng
D000911:Antibodies, Monoclonal; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D002681:China; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics*; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D005805:Genes, MHC Class I / Q000235:genetics; D015395:Histocompatibility Antigens Class I / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged; D010641:Phenotype; D019139:Tumor Escape / Q000235:genetics
D000911:Antibodies, Monoclonal; D015395:Histocompatibility Antigens Class I