Immunomodulation of bone marrow macrophages by GLIS, a proteoglycan fraction from Lingzhi or Reishi medicinal mushroom Ganoderma lucidium (W.Curt.:Fr.) P. Karst.
The immunomodulatory effect of GLIS (Lingzhi or Reishi medicinal mushroom Ganoderma lucidum immunomodulating substance) on macrophages has been investigated as part of ongoing research into the anticancer properties of this mushroom. Proliferation of bone marrow macrophages (BMMs) was enhanced by GLIS in a dose-dependent manner. Microscopic examination revealed that numerous GLIS-treated BMMs were enlarged and formed pseudopodia. Exposure of BMMs to GLIS resulted in significant increases in NO production, induction of cellular respiratory burst activity, and increased levels of IL-1β, IL-6, IL-12p35, IL-12p40, IL-18, and TNF-α gene expression and levels of TNF-α, IL-1β, and IL-12 secretion. Our data indicate that GLIS activates the immune system by modulating cytokine production.
National Engineering Research Center of Edible Fungi, China.
Zhe
Z
Ji
Qingjiu
Q
Tang
Jingsong
J
Zhang
Yan
Y
Yang
Yanfang
Y
Liu
Ying-Jie
YJ
Pan
D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects*; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology; D056747:Immunomodulation; D051379:Mice; D008807:Mice, Inbred BALB C; D011509:Proteoglycans / Q000737:chemistry* / Q000494:pharmacology*; D020940:Reishi / Q000737:chemistry*; D016897:Respiratory Burst / Q000187:drug effects; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013047:Specific Pathogen-Free Organisms
D016207:Cytokines; D011509:Proteoglycans