Gastric cytoprotection and honey intake in albino rats.
Beneficial effect of honey has been widely reported particularly on wound healings, gastrointestinal disorders and as antibacterial agent. However, there is paucity of report on its cytoprotective effect on the gastric mucosa despite its common usage worldwide including Nigeria. This study was therefore carried out to evaluate the effect of this widely consumed substance on gastric mucosa using animal model and also to explore possible mechanism of its action on the gastric mucosa .Twenty male adult albino rats of Wistar strain, weighing between 210-220g were used in the experiment. They were randomly assigned into two groups, the control group and the honey-fed (test) group, each containing ten rats. The Control group was fed on normal rat feed and water while the test group was fed on normal rat feed with honey added to its drinking water (1ml of honey for every initial 10ml of water for each rat daily) for twenty two weeks. After twenty two weeks the rats were weighed after being starved overnight. They were anaesthetized with urethane (0.6ml/100g body weight). Gastric ulceration was induced using 1.5ml acid-alcohol prepared from equivolume of 0.1NHCl and 70% methanol introduced into the stomach via a portex cannula tied and left in place following an incision made on the antral-pyloric junction of the stomach. The acid-alcohol was allowed to stay for 1hr. After 1hr, laparatomy was performed and the stomach isolated, cut open along the greater curvature, rinsed with normal saline and fastened in place with pins on a dissecting board for ulcer examination and scores. The result obtained showed mean ulcer scores of 14.5+/-0.70 for the control group and 1.6+/-0.11 for the test group. The result showed that honey significantly reduced ulcer scores as well as caused scanty haemorrhage in the test group compared with increased ulcer scores and multiple haemorrhage in the control group. It is therefore concluded that honey intake offered cytoprotection on the gastric mucosa of albino rats.
College of Medicine and Health Sciences, Imo State University, Owerri. chinayo58@yahoo.ca
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D000417:Albinism / Q000235:genetics; D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology*; D019610:Cytoprotection; D004195:Disease Models, Animal; D005753:Gastric Mucosa / Q000187:drug effects* / Q000473:pathology; D006722:Honey; D006851:Hydrochloric Acid; D008297:Male; D000432:Methanol; D010438:Peptic Ulcer Hemorrhage / Q000517:prevention & control; D051381:Rats; D017208:Rats, Wistar; D012720:Severity of Illness Index; D013276:Stomach Ulcer / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D013997:Time Factors
D000897:Anti-Ulcer Agents; D006851:Hydrochloric Acid; D000432:Methanol