Development of an accelerated solvent extraction, ultrasonic derivatisation LC-MS/MS method for the determination of the marker residues of nitrofurans in freshwater fish.
A rapid method using accelerated solvent extraction (ASE) and ultrasound enhanced derivatisation has been developed for the quantitative determination of metabolites of nitrofurans, namely 3-amino-2-oxalidinone (AOZ), 5-morpholinomethyl-3-amino-2-oxalidinone (AMOZ), 1-amino-hydantoin (AHD) and semicarbazide (SEM), in muscle and skin of carp and finless eel. The target analytes were extracted using ASE, ultrasonic derivatisation for 1 h and then purified by solid phase extraction. Averaged decision limits (CCα) and detection capability (CCβ) of the method were in the range of 0.07-0.13 and 0.31-0.49 µg kg⁻¹ in carp and finless eel, respectively. The accuracy in terms of recovery was in the range 77.2-97.4%. The simplified and traditional methods were compared with incurred residue samples. The simplified method reduced the derivatisation time and has been applied to the determination of nitrofurans residues in fish.
National Reference Laboratory of Veterinary Drug Residues-HZAU/MOA-Key Laboratory of Food Safety Evaluation, Huazhong Agricultural University, Wuhan 430070, China.
Yanfei
Y
Tao
Dongmei
D
Chen
Huimin
H
Wei
Pan
P
Yuanhu
Zhenli
Z
Liu
Lingli
L
Huang
Yulian
Y
Wang
Shuyu
S
Xie
Zonghui
Z
Yuan
D053000:Analytic Sample Preparation Methods; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D017756:Aquaculture; D002347:Carps / Q000254:growth & development; D002681:China; D002851:Chromatography, High Pressure Liquid; D004350:Drug Residues / Q000032:analysis*; D004524:Eels / Q000254:growth & development; D005396:Fish Products / Q000032:analysis*; D005506:Food Contamination; D005618:Fresh Water; D057230:Limit of Detection; D018482:Muscle, Skeletal / Q000737:chemistry; D009581:Nitrofurans / Q000032:analysis*; D012867:Skin / Q000737:chemistry; D052616:Solid Phase Extraction; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D014465:Ultrasonics / Q000379:methods
D000900:Anti-Bacterial Agents; D009581:Nitrofurans