Hydrodynamic shearing of DNA in a polymeric microfluidic device.
With the advent of next-generation sequencing (NGS) systems and the associated high throughput they afford, the input to these machines requires manageable lengths of fragments (~1000 bp) produced from chromosomal DNAs. Therefore, it is critical to develop devices that can shear DNA in a controlled fashion. We report a polymer-based microfluidic device that establishes an efficient and inexpensive platform with performance comparable to a commercially available bench-top system.
Department of Chemistry, Louisiana State University, Baton Rouge, LA 70820, USA.
Irina V
IV
Nesterova
Mateusz L
ML
Hupert
Malgorzata A
MA
Witek
Steven A
SA
Soper
D010582:Bacteriophage lambda / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D004279:DNA, Viral / Q000737:chemistry; D004867:Equipment Design; D006801:Humans; D057446:Hydrodynamics; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D013314:Stress, Mechanical
D004279:DNA, Viral; D004247:DNA