Biochemical evaluation of a synthesized isoflavone-selenium complex by molecular spectra.
A coordination compound of 5, 7-dihydrox-4'-methoxyisoflavone and selenium was synthesized and its structure was identified by IR, LC-MS and (1)H-NMR. Its biochemical effects were investigated using bovine serum albumin (BSA) as a target protein molecule, in which process three-dimensional (3D) fluorescence spectra, ultraviolet spectra, circular dichroism (CD) spectra and fluorescence probe techniques were employed. The interaction of SEIF and BSA was discussed by fluorescence quenching method and Förster non-radiation energy transfer theory. The thermodynamic parameters ΔH (θ), ΔG (θ), ΔS (θ) at different temperatures were calculated according to Van't Hoff isobaric equation and the results indicated the interaction was an exothermic as well as a spontaneous process. The binding site was explored by fluorescence probe method using warfarin and ibuprofen as markers. Intramolecular forces which are responsible for maintaining the binding were mainly hydrogen bond and van der Waals power. The average distance from the tryptophan residue in domain II of BSA (donor) to SEIF (acceptor) is 3.57 nm at body temperature. The conformation changes of BSA were investigated by 3D fluorescence and CD spectra.
College of Chemical and Pharmaceutical Engineering, Jingchu University of Technology, Jingmen, 448000 Hubei, People's Republic of China. h.x.zhang@yeah.net
Hua-xin
HX
Zhang
Zhen-xia
ZX
Huang
D000418:Albumins / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D007052:Ibuprofen / Q000378:metabolism; D007529:Isoflavones / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D016566:Organoselenium Compounds / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D018036:Selenium Compounds / Q000737:chemistry / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism*; D013816:Thermodynamics; D014859:Warfarin / Q000378:metabolism
D000418:Albumins; D007529:Isoflavones; D016566:Organoselenium Compounds; D018036:Selenium Compounds; D012710:Serum Albumin, Bovine; D014859:Warfarin; D007052:Ibuprofen