Recombination adenovirus-mediated human lactoferrin cDNA inhibits the growth of human MCF-7 breast cancer cells.
OBJECTIVE
Human lactoferrin, an 80 kDa iron-binding glycoprotein, has antitumour effects. We have explored the potential therapeutic role of re-expressing human lactoferrin gene product in human breast cancer.


METHODS
A recombinant adenovirus expressing the human lactoferrin cDNA (ad-hLTF) was constructed and used to infect breast cancer cells.


RESULTS
Seventy-two hours after infection, ad-hLTF had considerable cytotoxicity on MCF-7 cells. A time-course study showed that ad-hLTF infection of MCF-7 cells at 100 plaque-forming units per cell increased the number of cells in G(0) /G(1) phase and appeared markedly at Sub-G(1) apoptotic peak. The presence of apoptotic cells was confirmed using Annexin V-fluoresecein isothiocyanate apoptosis detection by flow cytometry. Ad-hLTF also resulted in a decrease of Bcl-2 protein and an increase in Bax protein.


CONCLUSIONS
Ad-hLTF plays an important role in the induction of cell cycle arrest and apoptosis in MCF-7 cells. The results demonstrated that ad-hLTF could have potential benefits in the treatment of breast cancer.
Department of Biological Engineering, College of Environment and Chemical Engineering, Yanshan University, Qinhuangdao, Japan.
Jianjie
J
Wang
Qingwang
Q
Li
Yetao
Y
Ou
Kun
K
Li
Zengsheng
Z
Han
Peijun
P
Wang
Shaobo
S
Zhou
D000256:Adenoviridae / Q000235:genetics*; D017209:Apoptosis / Q000187:drug effects*; D051017:Apoptosis Regulatory Proteins / Q000235:genetics; D000072224:Bcl-2-Like Protein 11; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000473:pathology; D053148:Caspase 3 / Q000235:genetics; D002453:Cell Cycle / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology; D015316:Genetic Therapy; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D007781:Lactoferrin / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics; D016133:Polymerase Chain Reaction; D011518:Proto-Oncogene Proteins / Q000235:genetics; D014407:Tumor Cells, Cultured; D051028:bcl-2-Associated X Protein / Q000235:genetics
D051017:Apoptosis Regulatory Proteins; C000606775:BCL2L11 protein, human; D000072224:Bcl-2-Like Protein 11; D018076:DNA, Complementary; D008565:Membrane Proteins; D011518:Proto-Oncogene Proteins; D051028:bcl-2-Associated X Protein; D049452:Green Fluorescent Proteins; D007781:Lactoferrin; D053148:Caspase 3