The effect of temozolomide/poly(lactide-co-glycolide) (PLGA)/nano-hydroxyapatite microspheres on glioma U87 cells behavior.
In this study, we investigated the effects of temozolomide (TMZ)/Poly (lactide-co-glycolide)(PLGA)/nano-hydroxyapatite microspheres on the behavior of U87 glioma cells. The microspheres were fabricated by the "Solid/Water/Oil" method, and they were characterized by using X-Ray diffraction, scanning electron microscopy and differential scanning calorimetry. The proliferation, apoptosis and invasion of glioma cells were evaluated by MTT, flow cytometry assay and Transwell assay. The presence of the key invasive gene, α(V)β3 integrin, was detected by the RT-PCR and Western blot method. It was found that the temozolomide/PLGA/nano-hydroxyapatite microspheres have a significantly diminished initial burst of drug release, compared to the TMZ laden PLGA microspheres. Our results suggest they can significantly inhibit the proliferation and invasion of glioma cells, and induce their apoptosis. Additionally, α(V)β3 integrin was also reduced by the microspheres. These data suggest that by inhibiting the biological behavior of glioma cells in vitro, the newly designed temozolomide/PLGA/nano-hydroxyapatite microspheres, as controlled drug release carriers, have promising potential in treating glioma.
Departments of Neurosurgery, The First Affiliated Hospital of China Medical University, 155 Nanjingbei Street, Heping District, Shenyang 110001, China.
Dongyong
D
Zhang
School of Materials and Metallurgy, Northeastern University, No.11, Lane 3, WenHua Road, HePing District, Shenyang 110819, China.
Ang
A
Tian
School of Materials and Metallurgy, Northeastern University, No.11, Lane 3, WenHua Road, HePing District, Shenyang 110819, China.
Xiangxin
X
Xue
School of Materials and Metallurgy, Northeastern University, No.11, Lane 3, WenHua Road, HePing District, Shenyang 110819, China.
Mei
M
Wang
Departments of Neurosurgery, The First Affiliated Hospital of China Medical University, 155 Nanjingbei Street, Heping District, Shenyang 110001, China.
Bo
B
Qiu
Departments of Neurosurgery, The First Affiliated Hospital of China Medical University, 155 Nanjingbei Street, Heping District, Shenyang 110001, China.
Anhua
A
Wu
D018906:Antineoplastic Agents, Alkylating / Q000737:chemistry* / Q000633:toxicity; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D003606:Dacarbazine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000633:toxicity; D004337:Drug Carriers / Q000737:chemistry; D017886:Durapatite / Q000737:chemistry*; D005910:Glioma / Q000378:metabolism / Q000473:pathology; D006801:Humans; D039302:Integrin alphaVbeta3 / Q000378:metabolism; D019344:Lactic Acid / Q000737:chemistry*; D008863:Microspheres; D049329:Nanostructures / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D000077204:Temozolomide
D018906:Antineoplastic Agents, Alkylating; D004337:Drug Carriers; D039302:Integrin alphaVbeta3; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D003606:Dacarbazine; D017886:Durapatite; D000077204:Temozolomide