Proanthocyanidin diversity in the EU 'HealthyHay' sainfoin (Onobrychis viciifolia) germplasm collection.
This study investigated 37 diverse sainfoin (Onobrychis viciifolia Scop.) accessions from the EU 'HealthyHay' germplasm collection for proanthocyanidin (PA) content and composition. Accessions displayed a wide range of differences: PA contents varied from 0.57 to 2.80 g/100 g sainfoin; the mean degree of polymerisation from 12 to 84; the proportion of prodelphinidin tannins from 53% to 95%, and the proportion of trans-flavanol units from 12% to 34%. A positive correlation was found between PA contents (thiolytic versus acid-butanol degradation; P<0.001; R(2)=0.49). A negative correlation existed between PA content (thiolysis) and mDP (P<0.05; R(2)=-0.30), which suggested that accessions with high PA contents had smaller PA polymers. Cluster analysis revealed that European accessions clustered into two main groups: Western Europe and Eastern Europe/Asia. In addition, accessions from USA, Canada and Armenia tended to cluster together. Overall, there was broad agreement between tannin clusters and clusters that were based on morphological and agronomic characteristics.
Chemistry and Biochemistry Laboratory, Food Production and Quality Division, School of Agriculture, Policy and Development, University of Reading, 1 Earley Gate, Reading RG6 6AT, UK. e.stringano@gmail.com
Elisabetta
E
Stringano
Christine
C
Hayot Carbonero
Lydia M J
LM
Smith
Ronald H
RH
Brown
Irene
I
Mueller-Harvey
D016000:Cluster Analysis; D005062:European Union; D007887:Fabaceae / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D044945:Proanthocyanidins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D044945:Proanthocyanidins; C013221:proanthocyanidin