Purification, cloning, and immunological characterization of arginine kinase, a novel allergen of Octopus fangsiao.
Arginine kinase (AK) is an important enzyme participating in energy metabolism in invertebrates, but, to date, there have been no reports that AK from octopus is an allergen. In this study, octopus AK was purified, and its molecular biological, immunological, and physicochemical characterizations were analyzed. The results showed that octopus AK was purified and confirmed by mass spectrometry for the first time, and its molecular mass was 38 kDa. The full-length gene sequence of octopus AK encompassed 1209 bp and was predicted to encode a protein with 348 amino acid residues. The homology of octopus AK and crustacean AK was about 54%, but the similarity between their three-dimensional structures was high. Octopus AK could react with mouse anti-shrimp AK and rabbit anti-crab AK polyclonal antibody singly. Octopus AK could also react with specific IgE of the sera from octopus-allergic patients effectively, whereas crab AK could inhibit the reaction between them. Finally, the IgE-binding activity of octopus AK could be reduced in the processes of thermal or acid-alkali treatment. In summary, AK was identified as a novel allergen in octopus, which had a sensitizing ability similar to that of crustacean AK. This is significant in allergy diagnosis and the treatment of octopus-allergic disorders.
College of Biological Engineering, The Key Laboratory of Science and Technology for Aquaculture and Food Safety, Jimei University, Xiamen, Fujian, China.
Hai-Wang
HW
Shen
Min-Jie
MJ
Cao
Qiu-Feng
QF
Cai
Mi-Mi
MM
Ruan
Hai-Yan
HY
Mao
Wen-Jin
WJ
Su
Guang-Ming
GM
Liu
D000485:Allergens; D000595:Amino Acid Sequence; D000818:Animals; D001122:Arginine Kinase / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D001483:Base Sequence; D055598:Chemical Phenomena; D003001:Cloning, Molecular; D005512:Food Hypersensitivity / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D008969:Molecular Sequence Data; D049831:Octopodiformes / Q000201:enzymology* / Q000276:immunology; D017747:Seafood / Q000032:analysis; D016415:Sequence Alignment
D000485:Allergens; D007073:Immunoglobulin E; D001122:Arginine Kinase