Phagocytosis and intracellular killing of Candida albicans by murine polymorphonuclear neutrophils.
Polymorphonuclear neutrophils (PMNs) are important phagocytes in the control of Candida infections. The phagocytic contribution of PMNs to host defence can by assessed by various methods, such as microbiological assays. However, assessment and definition of intracellular killing capacity can be a source of considerable confusion. A comparison of the growth of Candida in the presence of PMN with the growth of Candida in phagocyte-free suspensions may lead to an overestimation of killing capacity because PMNs can use both intracellular and extracellular killing mechanisms. Here, we describe the use of an adherent monolayer of exudate peritoneal PMNs that is used to differentiate between the process of phagocytosis and intracellular killing.
Department of Medical Microbiology and Infectious Diseases, University Medical Center Rotterdam, Rotterdam, The Netherlands. a.vonk@erasmusmc.nl
Alieke G
AG
Vonk
Mihai G
MG
Netea
Bart Jan
BJ
Kullberg
D000818:Animals; D002176:Candida albicans / Q000166:cytology* / Q000276:immunology*; D051379:Mice; D050296:Microbial Viability; D009504:Neutrophils / Q000166:cytology / Q000276:immunology*; D010587:Phagocytosis