Following enzyme activity with infrared spectroscopy.
Fourier transform infrared (FTIR) spectroscopy provides a direct, "on-line" monitor of enzymatic reactions. Measurement of enzymatic activity is based on the fact that the infrared spectra of reactants and products of an enzymatic reaction are usually different. Several examples are given using the enzymes pyruvate kinase, fumarase and alcohol dehydrogenase. The main advantage of the infrared method is that it observes the reaction of interest directly, i.e., no activity assay is required to convert the progress of the reaction into an observable quantity.
Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden. saroj@dbb.su.se
Saroj
S
Kumar
Andreas
A
Barth
D000426:Alcohol Dehydrogenase / Q000032:analysis / Q000378:metabolism*; D001681:Biological Assay / Q000379:methods*; D004789:Enzyme Activation; D005649:Fumarate Hydratase / Q000032:analysis / Q000378:metabolism*; D011770:Pyruvate Kinase / Q000032:analysis / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
D000426:Alcohol Dehydrogenase; D011770:Pyruvate Kinase; D005649:Fumarate Hydratase