Ribozyme-mediated trans insertion-splicing into target RNAs.
The trans insertion-splicing (TIS) reaction is a technique that can be used to site-specifically insert an RNA donor substrate into a separate RNA acceptor substrate. The TIS reaction, which is catalyzed by a group I intron-derived ribozyme from Pneumocystis carinii, is described with regards to system design, ribozyme preparation, and the overall protocol for conducting the TIS reaction.
Department of Physiology, University of Kentucky, Lexington, KY, USA.
P Patrick
PP
Dotson
Jonathan
J
Hart
Christopher
C
Noe
Stephen M
SM
Testa
D001665:Binding Sites; D005818:Genetic Engineering / Q000379:methods*; D009843:Oligoribonucleotides / Q000235:genetics / Q000378:metabolism; D045363:Pneumocystis carinii / Q000201:enzymology; D012326:RNA Splicing; D016337:RNA, Catalytic / Q000235:genetics* / Q000378:metabolism*; D013379:Substrate Specificity
D009843:Oligoribonucleotides; D016337:RNA, Catalytic