Study of nano-fiber cellulose production by Glucanacetobacter xylinum ATCC 10245.
Bacterial Celluloses (BC) are gaining importance in research and commerce due to numerous factors affecting the bacterial cellulose characteristics and application in different industries. The aim of the present study was to produce bacterial cellulose in different media using different cultivation vessels. Bacterial cellulose was produced by static cultivation of Glucanacetobacter xylinum ATCC 10245 in different culture media such as Brain Heart Agar, Luria Bertani Agar /Broth, Brain Heart Infusion, Hestrin-Schramm and medium no. 125. Cultivation of bacterium was conducted in various culture vessels with different surface area. The cellulose membrane was treated and purified with a 0.1 M NaOH solution at 90 degreesC for 30 min and dried by a freeze- drier at -40 degreesC to obtain BC. The prepared bacterial cellulose was characterized by scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) spectroscopy and X-ray diffraction (XRD). The amount of produced BC was related directly to the surface area of culture vessels.
Department of Pilot Biotechnology, Pasteur Institute of Iran, Iran.
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D
Norouzian
A
A
Farhangi
S
S
Tolooei
Z
Z
Saffari
M R
MR
Mehrabi
M
M
Chiani
S
S
Ghassemi
M
M
Farahnak
A
A
Akbarzadeh
D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000737:chemistry*; D002482:Cellulose / Q000096:biosynthesis* / Q000737:chemistry*; D041822:Gluconacetobacter xylinus / Q000737:chemistry / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
D001426:Bacterial Proteins; D002482:Cellulose