Comparative transcriptomics and proteomics analysis of citrus fruit, to improve understanding of the effect of low temperature on maintaining fruit quality during lengthy post-harvest storage.
Fruit quality is a very complex trait that is affected by both genetic and non-genetic factors. Generally, low temperature (LT) is used to delay fruit senescence and maintain fruit quality during post-harvest storage but the molecular mechanisms involved are poorly understood. Hirado Buntan Pummelo (HBP; Citrus grandis × C. paradis) fruit were chosen to explore the mechanisms that maintain citrus fruit quality during lengthy LT storage using transcriptome and proteome studies based on digital gene expression (DGE) profiling and two-dimensional gel electrophoresis (2-DE), respectively. Results showed that LT up-regulated stress-responsive genes, arrested signal transduction, and inhibited primary metabolism, secondary metabolism and the transportation of metabolites. Calcineurin B-like protein (CBL)-CBL-interacting protein kinase complexes might be involved in the signal transduction of LT stress, and fruit quality is likely to be regulated by sugar-mediated auxin and abscisic acid (ABA) signalling. Furthermore, ABA was specific to the regulation of citrus fruit senescence and was not involved in the LT stress response. In addition, the accumulation of limonin, nomilin, methanol, and aldehyde, together with the up-regulated heat shock proteins, COR15, and cold response-related genes, provided a comprehensive proteomics and transcriptomics view on the coordination of fruit LT stress responses.
Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, Hubei, P.R. China.
Ze
Z
Yun
Shuai
S
Jin
Yuduan
Y
Ding
Zhuang
Z
Wang
Huijun
H
Gao
Zhiyong
Z
Pan
Juan
J
Xu
Yunjiang
Y
Cheng
Xiuxin
X
Deng
D000040:Abscisic Acid / Q000378:metabolism / Q000494:pharmacology; D000383:Agriculture; D000693:Anaerobiosis / Q000187:drug effects / Q000235:genetics; D002241:Carbohydrates / Q000032:analysis / Q000494:pharmacology; D002264:Carboxylic Acids / Q000378:metabolism; D002957:Citrus / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism* / Q000502:physiology; D003080:Cold Temperature; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling / Q000379:methods*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics; D007210:Indoleacetic Acids / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011309:Preservation, Biological; D040901:Proteomics / Q000379:methods*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D012995:Solubility / Q000187:drug effects; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics; D013997:Time Factors; D059467:Transcriptome / Q000187:drug effects / Q000235:genetics*
D002241:Carbohydrates; D002264:Carboxylic Acids; D007210:Indoleacetic Acids; D010940:Plant Proteins; D012333:RNA, Messenger; D000040:Abscisic Acid