Analytical performance of commercially-available assays for feline insulin-like growth factor 1 (IGF-1), adiponectin and ghrelin measurements.
The objective of this study was to evaluate three commercially-available human assays for the determination of adiponectin, ghrelin and insulin-like growth factor 1 (IGF-1) concentrations in feline serum samples. Intra- and interassay coefficients of variation were lower than 20%, 15% and 6% for adiponectin, ghrelin and IGF-1 assays, respectively. Dilutions of feline serum pools resulted in linear regression equations in all kits. Mean recovery of adiponectin, ghrelin and IGF-1 assays were 107%, 102% and 105%, respectively. Significant differences were detected in adiponectin and ghrelin concentrations between lean and obese cats (P <0.05 in both cases), but there was no difference in IGF-1 concentrations (P = 0.12).
Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine University of Murcia, Spain.
Asta
A
Tvarijonaviciute
Alexander J
AJ
German
Silvia
S
Martínez-Subiela
Fernando
F
Tecles
Jose J
JJ
Ceron
D052242:Adiponectin / Q000097:blood*; D000818:Animals; D002371:Cat Diseases / Q000097:blood; D002415:Cats / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards / Q000662:veterinary*; D054439:Ghrelin / Q000097:blood*; D006461:Hemolysis / Q000502:physiology; D006932:Hyperbilirubinemia / Q000097:blood / Q000662:veterinary; D006949:Hyperlipidemias / Q000097:blood / Q000662:veterinary; D007334:Insulin-Like Growth Factor I / Q000032:analysis*; D009765:Obesity / Q000097:blood / Q000662:veterinary; D011237:Predictive Value of Tests; D012044:Regression Analysis; D014280:Triglycerides / Q000097:blood
D052242:Adiponectin; D054439:Ghrelin; D014280:Triglycerides; D007334:Insulin-Like Growth Factor I