Application of click chemistry to the production of DNA microarrays.
The copper-catalyzed alkyne-azide cycloaddition (CuAAC) reaction was applied as the novel method of DNA immobilization on a modified solid support. The CuAAC click reaction enables the covalent binding of DNA modified with pentynyl groups at its 5'-end to azide-loaded slides. Click microarrays were produced using this approach and successfully employed in biological/model experiments.
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań, Poland.
Barbara
B
Uszczyńska
Tomasz
T
Ratajczak
Emilia
E
Frydrych
Hieronim
H
Maciejewski
Marek
M
Figlerowicz
Wojciech T
WT
Markiewicz
Marcin K
MK
Chmielewski
D000480:Alkynes / Q000737:chemistry*; D001386:Azides / Q000737:chemistry*; D001483:Base Sequence; D002384:Catalysis; D057930:Click Chemistry / Q000379:methods*; D003300:Copper / Q000737:chemistry; D003500:Cyclization; D004247:DNA / Q000138:chemical synthesis / Q000737:chemistry*; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation*
D000480:Alkynes; D001386:Azides; D003300:Copper; D004247:DNA