A FRET-based probe with a chemically deactivatable quencher.
A new concept of a chemically deactivatable quencher is proposed for a FRET-based probe that turns-on its fluorescence by either an enzymatic cleavage or a chemical reagent (sodium dithionite). This concept allowed us to quantify the caspase-3 cleavage activity in solution and to reveal unreacted probes in cell experiments.
Laboratory of Functional Chemo-Systems UMR 7199, 74 Route du Rhin, 67401, Illkirch, France.
Geoffray
G
Leriche
Ghyslain
G
Budin
Zeinab
Z
Darwich
Denis
D
Weltin
Yves
Y
Mély
Andrey S
AS
Klymchenko
Alain
A
Wagner
D053148:Caspase 3 / Q000737:chemistry / Q000378:metabolism; D004227:Dithionite / Q000737:chemistry; D005453:Fluorescence; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D015394:Molecular Structure; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism
D005456:Fluorescent Dyes; D011994:Recombinant Proteins; D004227:Dithionite; D053148:Caspase 3