Carbon source pulsed feeding to attain high yield and high productivity in poly(3-hydroxybutyrate) (PHB) production from soybean oil using Cupriavidus necator.
Poly(3-hydroxybutyrate) (PHB) biosynthesis from soybean oil by Cupriavidus necator was studied using a bench scale bioreactor. The highest cell concentration (83 g l(-1)) was achieved using soybean oil at 40 g l(-1) and a pulse of the same concentration. The PHB content was 81% (w/w), PHB productivity was 2.5 g l(-1) h(-1), and the calculated Y(p/s) value was 0.85 g g(-1). Growth limitation and the onset of PHB biosynthesis took place due to exhaustion of P, and probably also Cu, Ca, and Fe.
Laboratório Nacional de Ciência e Tecnologia do Bioetanol, Centro Nacional de Pesquisa em Energia e Materiais, Campinas, SP, Brazil. jpradella@bioetanol.org.br
José Geraldo da Cruz
JG
Pradella
Jaciane Lutz
JL
Ienczak
Cecília Romero
CR
Delgado
Marilda Keico
MK
Taciro
D019149:Bioreactors; D002244:Carbon / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D020118:Cupriavidus necator / Q000254:growth & development / Q000378:metabolism*; D006885:Hydroxybutyrates / Q000378:metabolism*; D008670:Metals / Q000378:metabolism; D011091:Polyesters / Q000378:metabolism*; D013024:Soybean Oil / Q000378:metabolism*; D000094122:Polyhydroxybutyrates
D002244:Carbon; D003470:Culture Media; D006885:Hydroxybutyrates; D008670:Metals; D011091:Polyesters; D013024:Soybean Oil; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates