Facile construction of functional biosurface via SI-ATRP and "click glycosylation".
Construction of high density glycosylated surfaces is important in the investigation of interactions between pathogens and surface carbohydrates. In this work, we provided a flexible method for glycosyl surface fabrication by combination of surface-initiated atom transfer radical polymerization (SI-ATRP) and copper-catalyzed azide-alkyne 1,3-dipolar cycloaddition (CuAAC) reaction. Through this strategy, we got a very high surface glycosyl density of about 4 nmol/cm(2) with the surface "click" efficiency of nearly 50%. Then the carbohydrate decorated surfaces were used to mimic cell surfaces and specific recognition of mannose with Escherichia coli was observed. We believe the methodology provided here can be used as a facile way for construction of a wide range of functional biosurfaces.
Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, PR China.
Wantong
W
Song
Chunsheng
C
Xiao
Liguo
L
Cui
Zhaohui
Z
Tang
Xiuli
X
Zhuang
Xuesi
X
Chen
D000480:Alkynes / Q000737:chemistry; D001386:Azides / Q000737:chemistry; D001422:Bacterial Adhesion / Q000502:physiology*; D057930:Click Chemistry; D003300:Copper / Q000737:chemistry; D004926:Escherichia coli / Q000502:physiology; D005690:Galactose / Q000737:chemistry*; D006031:Glycosylation; D054884:Host-Pathogen Interactions; D008358:Mannose / Q000737:chemistry*; D008567:Membranes, Artificial; D018625:Microscopy, Atomic Force; D008872:Microwaves; D058105:Polymerization; D011108:Polymers / Q000737:chemistry; D012825:Silicon / Q000737:chemistry; D013499:Surface Properties
D000480:Alkynes; D001386:Azides; D008567:Membranes, Artificial; D011108:Polymers; D003300:Copper; D008358:Mannose; D005690:Galactose; D012825:Silicon