Immunohistochemical markers for tumor associated macrophages and survival in advanced classical Hodgkin's lymphoma.
A subset of patients with advanced classical Hodgkin's lymphoma is refractory to standard therapies. Therefore, it is relevant to identify new biologically-based prognostic markers. Recently, tumor associated macrophages have been proposed as a factor that predicts survival, although contradictory results have also been reported. Here we analyzed four macrophage markers (CD68, CD163, LYZ, and STAT1) using immunohistochemistry and automated quantification, in two independent series of advanced classical Hodgkin's lymphoma (n=266 and 103 patients, respectively). Our results did not confirm that specific macrophage immunohistochemical markers could be used as surrogates for gene expression profiling studies. Survival analyses did not show correlation between CD163, LYZ or STAT1 and either failure-free or disease-specific survival. There was an association between CD68 and disease-specific survival, but it was not consistent in both series. In conclusion, individual tumor associated macrophage markers cannot be used to predict outcome before technical standardization and prospective validation in independent series of patients with comparable stages and treatments.
Department of Pathology, M.D. Anderson Cancer Center, Houston, TX, USA.
Beatriz
B
Sánchez-Espiridión
Ana M
AM
Martin-Moreno
Carlos
C
Montalbán
L Jeffrey
LJ
Medeiros
Francisco
F
Vega
Anas
A
Younes
Miguel A
MA
Piris
Juan F
JF
Garcia
D000328:Adult; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000235:genetics / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D006689:Hodgkin Disease / Q000175:diagnosis* / Q000401:mortality / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008264:Macrophages / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009113:Muramidase / Q000235:genetics / Q000378:metabolism; D011237:Predictive Value of Tests; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D050794:STAT1 Transcription Factor / Q000235:genetics / Q000378:metabolism; D012720:Severity of Illness Index; D016019:Survival Analysis; D000098902:CD163 Antigen; D000097382:CD68 Molecule
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D014408:Biomarkers, Tumor; D009113:Muramidase; D011956:Receptors, Cell Surface; D050794:STAT1 Transcription Factor; D000098902:CD163 Antigen; C067980:CD68 antigen, human; D000097382:CD68 Molecule; C494079:STAT1 protein, human