Anti-adipogenic activity of Cordyceps militaris in 3T3-L1 cells.
Inhibition of adipocytes differentiation is suggested to be an important strategy for prevention and/or treatment of obesity. In our present study, Cordyceps militaris showed significant inhibitory activity on adipocyte differentiation in 3T3-L1 preadipocytes as assessed by measuring fat accumulation using Oil Red O staining. Activity-guided fractionation led to the isolation of cordycepin (1), guanosine (2) and tryptophan (3) as active compounds. All the three compounds were more effective in the prevention of early stage of adipogenesis than in lipolysis. In addition, combinational treatment of three compounds significantly increased anti-adipogenic activity.
College of Pharmacy, Chungbuk National University, Cheongju 361-763, Korea.
Qing
Q
Liu
In Pyo
IP
Hong
Mi-Jeong
MJ
Ahn
Hwan-Soo
HS
Yoo
Sang-Bae
SB
Han
Bang Yeon
BY
Hwang
Mi Kyeong
MK
Lee
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D032661:Cordyceps; D003839:Deoxyadenosines / Q000494:pharmacology; D006151:Guanosine / Q000494:pharmacology; D008066:Lipolysis / Q000187:drug effects; D051379:Mice; D013329:Structure-Activity Relationship; D014364:Tryptophan / Q000494:pharmacology
D003839:Deoxyadenosines; D006151:Guanosine; D014364:Tryptophan; C058120:cordycepin