Thermovum composti gen. nov., sp. nov., an alphaproteobacterium from compost.
A Gram-stain-positive thermophilic bacterium, designated strain Nis3(T), was isolated from compost. The strain grew at 23-57 °C (optimum, 50 °C); no growth was observed below 15 or above 60 °C. The pH range for growth was 5.9-8.8 (optimum, 7.0); no growth was observed below pH 5.4 or above pH 9.3. The DNA G+C content of strain Nis3(T) was 63.4 mol%. The dominant quinone type was ubiquinone Q-10. The major fatty acids were C(18:1)ω7c, C(19:0)ω8c cyclo and C(18:0). The polar lipids comprised phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylinositol, phosphatidylmonomethylethanolamine, an unknown glycolipid and a ninhydrin-positive phospholipid. 16S rRNA gene sequence analysis assigned this bacterium to the family Phyllobacteriaceae in the Alphaproteobacteria but it shared less than 95.2% sequence similarity with other members of the family. The chemotaxonomic and phenotypic characteristics of strain Nis3(T) differed in some respects from those of members of the family Phyllobacteriaceae. Therefore, strain Nis3(T) is considered to represent a novel species of a new genus in the family Phyllobacteriaceae, for which the name Thermovum composti gen. nov., sp. nov. is proposed. The type strain is Nis3(T) ( = JCM 17863(T) = KCTC 23707(T)).
Hazaka Plant Research Center, Kennan Eisei Kogyo Co. Ltd, 44 Aza-Inariyama, Oaza-Ashitate, Murata-cho, Shibata-gun, Miyagi 989-1311, Japan.
Shuhei
S
Yabe
Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi Bunkyo-ku, Tokyo 113-0032, Japan.
Yoshifumi
Y
Aiba
Hazaka Plant Research Center, Kennan Eisei Kogyo Co. Ltd, 44 Aza-Inariyama, Oaza-Ashitate, Murata-cho, Shibata-gun, Miyagi 989-1311, Japan.
Yasuteru
Y
Sakai
Hazaka Plant Research Center, Kennan Eisei Kogyo Co. Ltd, 44 Aza-Inariyama, Oaza-Ashitate, Murata-cho, Shibata-gun, Miyagi 989-1311, Japan.
Masaru
M
Hazaka
Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi Bunkyo-ku, Tokyo 113-0032, Japan.
Akira
A
Yokota
D015373:Bacterial Typing Techniques; D001482:Base Composition; D005227:Fatty Acids / Q000032:analysis; D007564:Japan; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D059127:Phyllobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012987:Soil; D012988:Soil Microbiology
D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D012987:Soil