A dynamic perspective of RNAi library development.
Shortly after the dissertation of the mechanism of RNA interference (RNAi), various RNAi libraries for invertebrates, plants or mammals that enable loss-of-function genetic screens on a genome-wide scale have been developed. Joint academic and industrial effort has led to the commercial launch of many of these libraries and this field is expected to continuously evolve at incredible speed. This article comparatively reviews the principles and applications of different RNAi libraries: from earlier synthetic to recent lentiviral RNAi libraries. The unique properties and limitations of each library will be important references for instigators to choose a particular library for their specific application.
Department of Gastroenterology and Hepatology, Erasmus MC-University Medical Center, Rotterdam, The Netherlands.
Qiuwei
Q
Pan
Luc J W
LJ
van der Laan
Harry L A
HL
Janssen
Maikel P
MP
Peppelenbosch
D000818:Animals; D035843:Biomedical Research; D030561:Databases, Nucleic Acid; D006801:Humans; D051379:Mice; D034622:RNA Interference; D034741:RNA, Small Interfering; D054852:Small Molecule Libraries
D034741:RNA, Small Interfering; D054852:Small Molecule Libraries