Capture of cell-derived microvesicles (exosomes and apoptotic bodies) by human plasmacytoid dendritic cells.
cDCs and pDCs differ in multiple aspects. Among those, antigen capture is a recognized feature of cDCs, whereas pDCs display poor capacity to capture cell-derived antigens. However, animal models of organ transplantation suggested a role for pDCs in tolerance induction via phagocytosis of donor antigens. In a transplantation setting, microvesicles, such as apoptotic bodies and exosomes secreted by the graft, may be potential sources of alloantigen. Here, we tested the capacity of human pDCs to capture exosomes and apoptotic bodies from Jurkat T cells. Exosomes and apoptotic bodies were indeed captured by pDCs, although required longer times of incubation when compared with the highly endocytic cDCs. In cDCs and pDCs, exosome capture was more efficient than apoptotic bodies. Endocytosis inhibitors clearly impaired exosome capture by cDCs, although this could not be verified in pDCs as a result of cellular toxicity. Functionally, capture of Jurkat-derived exosomes did not induce nor prevent pDC maturation, and exosome-loaded pDCs induced T cell proliferation, suggesting a link between capture and presentation. Thus, exosomes and apoptotic bodies may be sources of antigen for human pDCs.
Institut d'Investigació Germans Trias i Pujol, Ctra. de Can Ruti, Camí de les Escoles s/n, 08916 Badalona (Barcelona), Spain.
Patricia
P
Bastos-Amador
Begoña
B
Pérez-Cabezas
Nuria
N
Izquierdo-Useros
Maria C
MC
Puertas
Javier
J
Martinez-Picado
Ricardo
R
Pujol-Borrell
Mar
M
Naranjo-Gómez
Francesc E
FE
Borràs
D017951:Antigen Presentation; D017209:Apoptosis / Q000502:physiology*; D055252:Cell-Derived Microparticles / Q000502:physiology*; D003713:Dendritic Cells / Q000502:physiology*; D004705:Endocytosis; D055354:Exosomes / Q000502:physiology*; D006801:Humans; D019169:Jurkat Cells; D008213:Lymphocyte Activation; D010587:Phagocytosis; D013601:T-Lymphocytes / Q000166:cytology*