Determination of trace vitamin C by ion-pair HPLC with UV detection in calcium gluconate and vitamin C compound oral solution.
A sensitive and specific reversed-phase high-performance liquid chromatographic method with ultraviolet detection was developed for the determination of vitamin C, using tetrabutylammonium hydroxide as an ion-pair reagent in a compound oral solution containing 100 mg/mL calcium gluconate and 1.25 mg/mL vitamin C. The aqueous phase contained 0.005 mol/L tetrabutylammonium hydroxide and the mobile phase consisted of a mixture of the aqueous phase-methanol (80:20, v/v, pH 6.0 adjusted by phosphoric acid). The linearity, sensitivity and specificity, accuracy, and stability of the procedure were evaluated. The calibration curves for vitamin C were linear in the range of 10.0-100.0 µg/mL. The percentage coefficient of variation of the quantitative analysis of the vitamin C in the products analysis was within 5%. The method was successfully applied to determine the stability of vitamin C in the compound oral solution. It was found that the vitamin C peak was symmetrical and the column efficiency was high. The method is simple and suitable for stability testing of a low concentration of vitamin C preparation.
College of Pharmaceutical Sciences, Hebei University, No.180, WuSi Road, Baoding, 071002, PR China. hbupharm@126.com
Liandong
L
Hu
Li
L
Li
Zhaoliang
Z
Luo
Jianxue
J
Yang
Wei
W
Liu
D000284:Administration, Oral; D001205:Ascorbic Acid / Q000032:analysis*; D002125:Calcium Gluconate / Q000032:analysis*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D057230:Limit of Detection; D015203:Reproducibility of Results; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
D012996:Solutions; D001205:Ascorbic Acid; D002125:Calcium Gluconate