Human fetal liver stromal cells expressing erythropoietin promote hematopoietic development from human embryonic stem cells.
Blood cells transfusion and hematopoietic stem cells (HSCs) transplantation are important methods for cell therapy. They are widely used in the treatment of incurable hematological disorder, infectious diseases, genetic diseases, and immunologic deficiency. However, their availability is limited by quantity, capacity of proliferation and the risk of blood transfusion complications. Recently, human embryonic stem cells (hESCs) have been shown to be an alternative resource for the generation of hematopoietic cells. In the current study, we describe a novel method for the efficient production of hematopoietic cells from hESCs. The stable human fetal liver stromal cell lines (hFLSCs) expressing erythropoietin (EPO) were established using the lentiviral system. We observed that the supernatant from the EPO transfected hFLSCs could induce the hESCs differentiation into hematopoietic cells, especially erythroid cells. They not only expressed fetal and embryonic globins but also expressed the adult-globin chain on further maturation. In addition, these hESCs-derived erythroid cells possess oxygen-transporting capacity, which indicated hESCs could generate terminally mature progenies. This should be useful for ultimately developing an animal-free culture system to generate large numbers of erythroid cells from hESCs and provide an experimental model to study early human erythropoiesis.
Stem Cell and Regenerative Medicine Lab, Beijing Institute of Transfusion Medicine, Beijing, People's Republic of China.
Chao
C
Yang
Lei
L
Ji
Wen
W
Yue
Shuang-Shuang
SS
Shi
Ruo-Yong
RY
Wang
Yan-Hua
YH
Li
Xiao-Yan
XY
Xie
Jia-Fei
JF
Xi
Li-Juan
LJ
He
Xue
X
Nan
Xue-Tao
XT
Pei
D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002460:Cell Line; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000502:physiology; D041905:Erythroid Cells / Q000166:cytology / Q000378:metabolism; D004921:Erythropoietin / Q000235:genetics / Q000378:metabolism*; D060747:Fetal Globulins / Q000378:metabolism; D005333:Fetus / Q000166:cytology*; D006410:Hematopoiesis / Q000502:physiology*; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D008099:Liver / Q000166:cytology* / Q000196:embryology / Q000378:metabolism; D017154:Stromal Cells / Q000166:cytology* / Q000378:metabolism*; D014162:Transfection
D017077:Culture Media, Conditioned; D060747:Fetal Globulins; D004921:Erythropoietin