Assessment of the genotoxicity of heavy metals in Phaseolus vulgaris L. as a model plant system by Random Amplified Polymorphic DNA (RAPD) analysis.
Impact assessments of environmental pollutants are important in eco-genotoxicology. A random amplified polymorphic DNA (RAPD) technique was used to detect genotoxicity-induced DNA damage in Phaseolus vulgaris L. from heavy metals at two different concentrations. The results from six 10-base pair (bp) random RAPD primers with 60-70% GC content used, showed a total of 295 RAPD fragments of 700-4000 bp in molecular size in the seedlings of untreated and treated samples, of which only 163 fragments were polymorphic. Polymorphisms became evident as the disappearance and/or appearance of DNA fragments in treated samples compared to the control. A dendrogram constructed using the Numerical Taxonomy and Multivariate Analysis System (NTSYSps), showed that the control group merged with groups treated with CuSO(4)·5H(2)O (150 mg L(-1)) and MnSO(4)·H(2)O (150 mg L(-1)) in a separate cluster. These groups were linked with all of the other samples treated with metals at concentrations of 150 mg L(-1) and CuSO(4)·5H(2)O and Cd(NO(3))(2) at concentrations of 350 mg L(-1). Finally, the samples treated with metals at concentrations of 350 mg L(-1) together with NiSO(4) at the concentration of 150 mg L(-1), clustered separately. The DNA polymorphism detected by RAPD analysis offered a useful biomarker assay for the detection of toxic chemicals genotoxicity in plant model systems.
Faculty of Medical Sciences, Goce Delčev University, Štip, Macedonia. darinka.gorgieva@ugd.edu.mk
Darinka
D
Gjorgieva
Tatjana
T
Kadifkova-Panovska
Saša
S
Mitrev
Biljana
B
Kovacevik
Emilija
E
Kostadinovska
Katerina
K
Bačeva
Trajče
T
Stafilov
D004249:DNA Damage; D018744:DNA, Plant / Q000187:drug effects; D019216:Metals, Heavy / Q000633:toxicity*; D009153:Mutagens / Q000633:toxicity*; D027805:Phaseolus / Q000187:drug effects* / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D036226:Seedlings / Q000187:drug effects / Q000235:genetics; D012989:Soil Pollutants / Q000633:toxicity*
D018744:DNA, Plant; D019216:Metals, Heavy; D009153:Mutagens; D012989:Soil Pollutants