Lactobacillus pasteurii sp. nov. and Lactobacillus hominis sp. nov.
Strains 1517(T) and 61D(T) were characterized by phenotypic and molecular taxonomic methods. These Gram-positive lactic acid bacteria were homo-fermentative, facultatively anaerobic short rods. They were phylogenetically related to the genus Lactobacillus according to 16S rRNA gene sequence analysis, with 99 % similarity between strain 1517(T) and the type strain of Lactobacillus gigeriorum, and 98.6, 98.5 and 98.4 % between strain 61D(T) and Lactobacillus gasseri, Lactobacillus taiwanensis and Lactobacillus johnsonii, respectively. Multilocus sequence analysis and metabolic analysis of both strains showed variation between the two strains and their close relatives, with variation in the position of the pheS and rpoA genes. The DNA-DNA relatedness of 43.5 % between strain 1517(T) and L. gigeriorum, and 38.6, 29.9 and 39.7 % between strain 61D(T) and L. johnsonii, L. taiwanensis and L. gasseri, respectively, confirmed their status as novel species. Based on phenotypic and genotypic characteristics, two novel species of Lactobacillus are proposed: Lactobacillus pasteurii sp. nov., with 1517(T) ( = CRBIP 24.76(T) = DSM 23907(T)) as the type strain, and Lactobacillus hominis sp. nov., with 61D(T) (=CRBIP 24.179(T) = DSM 23910(T)) as the type strain.
Institut Pasteur, Plate-forme Génomique, Dept Génomes et Génétique, F-75015 Paris, France.
Sylvie
S
Cousin
Institut Pasteur, CRBIP (Centre de Ressources Biologiques de l'Institut Pasteur), Dept Microbiologie, F-75015 Paris, France.
Laurence
L
Motreff
Institut Pasteur, CRBIP (Centre de Ressources Biologiques de l'Institut Pasteur), Dept Microbiologie, F-75015 Paris, France.
Marie-Laure
ML
Gulat-Okalla
Institut Pasteur, Plate-forme Génomique, Dept Génomes et Génétique, F-75015 Paris, France.
Catherine
C
Gouyette
DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (German Collection of Microorganisms and Cell Cultures), D-38124 Braunschweig, Germany.
Cathrin
C
Spröer
DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (German Collection of Microorganisms and Cell Cultures), D-38124 Braunschweig, Germany.
Peter
P
Schumann
Institut Pasteur, CRBIP (Centre de Ressources Biologiques de l'Institut Pasteur), Dept Microbiologie, F-75015 Paris, France.
Evelyne
E
Begaud
Institut Pasteur, Plate-forme Génomique, Dept Génomes et Génétique, F-75015 Paris, France.
Christiane
C
Bouchier
Institut Pasteur, CRBIP (Centre de Ressources Biologiques de l'Institut Pasteur), Dept Microbiologie, F-75015 Paris, France.
Dominique
D
Clermont
Institut Pasteur, CRBIP (Centre de Ressources Biologiques de l'Institut Pasteur), Dept Microbiologie, F-75015 Paris, France.
Chantal
C
Bizet
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D007778:Lactobacillus / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S