Molecular cloning and expression analysis of IFN-β promoter stimulator 1 in Tibetan pigs.
IFN-β promoter stimulator 1 (IPS-1) is an important adaptor protein linking RIG-I/MDA5 to the downstream signaling molecules and plays the pivotal role in type I interferons induction. In this study, we cloned and characterized Tibetan porcine IPS-1, investigated the tissue distribution, compared different messenger RNA expression for IPS-1 between Tibetan and Crossbred (Duroc × Yorkshire × Landrace) pigs (DLY). The Tibetan porcine IPS-1 gene was first cloned from spleen. The entire open reading frame (ORF) of the IPS-1 is 1,575 bp and encodes for 524 amino acid residues, has 1 putative transmembrane domains, with a higher degree of sequence similarity with common pig (99.37%) and cattle (81.23%) than with human (70.20%) or mouse (63.44%). Real-time quantitative PCR analysis indicated that Tibetan porcine IPS-1 mRNA was most abundant in the liver and kidney. The expression of IPS-1 of Tibetan pigs in most tissues was higher than DLY pigs.
Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an, Sichuan, People's Republic of China.
Ye
Y
Zhao
Bing
B
Yu
XiangBing
X
Mao
Guoquan
G
Han
Qian
Q
Mao
Zhiqing
Z
Huang
Daiwen
D
Chen
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D003001:Cloning, Molecular; D005260:Female; D007421:Intestine, Small / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D009928:Organ Specificity; D017422:Sequence Analysis, DNA; D034421:Sus scrofa / Q000235:genetics*; D014158:Transcription, Genetic
D048868:Adaptor Proteins, Signal Transducing