Embryonic stem cell strategies to explore neural crest development in human embryos.
Controling embryonic stem cell fate in vitro has been a major challenge in the past decade. Several protocols have been developed to obtain neural crest derivatives in culture, using more or less defined conditions. Here, we present various strategies used to date to obtain neural crest specification and the markers that can be used to identify human neural crest cells.
Institut Curie, INSERM U1021, CNRS, UMR 3347, F-91405 Orsay, France.
Cécile
C
Milet
Anne H
AH
Monsoro-Burq
D000818:Animals; D015415:Biomarkers; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D019070:Cell Lineage; D002465:Cell Movement; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology*; D061252:Feeder Cells; D006801:Humans; D009432:Neural Crest / Q000166:cytology* / Q000196:embryology
D015415:Biomarkers