Biological glucose metabolism regulated peptide self-assembly as a simple visual biosensor for glucose detection.
A glucose oxidase (GOx)-mediated glucose metabolism was in vitro mimicked and employed to regulate the self-assembly of peptide-based building blocks. In this new stimuli-responsive self-assembly system, two peptide-based building blocks, respectively, having aspartic acid (gelator 1) and lysine (gelator 2) residues were designed and prepared. When adding glucose and GOx to the aqueous solution of gelator 1 or the self-assembled fibrillar hydrogel of gelator 2 to construct glucose metabolism system, the metabolic product (gluconic acid) can trigger the protonation of the peptide molecules and induce the phase transitions of gelators 1 (sol-gel) and 2 (gel-sol). Because this glucose metabolism regulated peptide self-assembly is built on the oxidation of glucose, it can be used as a simple visual biosensor for glucose detection.
Key Laboratory of Biomedical Polymers of Ministry of Education, Department of Chemistry, Wuhan University, Wuhan, P. R. China.
Xiao-Ding
XD
Xu
Bing-Bing
BB
Lin
Jun
J
Feng
Ya
Y
Wang
Si-Xue
SX
Cheng
Xian-Zheng
XZ
Zhang
Ren-Xi
RX
Zhuo
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004800:Enzymes, Immobilized / Q000737:chemistry; D005942:Gluconates / Q000737:chemistry; D005947:Glucose / Q000737:chemistry* / Q000378:metabolism; D005949:Glucose Oxidase / Q000737:chemistry; D006801:Humans; D010084:Oxidation-Reduction; D010455:Peptides / Q000737:chemistry
D004800:Enzymes, Immobilized; D005942:Gluconates; D010455:Peptides; D005949:Glucose Oxidase; D005947:Glucose; C030691:gluconic acid