Human immunogenic T cell epitopes in nucleoprotein of human influenza A (H5N1) virus.
1. Two novel HLA-A2.1 specific H5N1 nucleoprotein epitopes(NP373-381 AMDSNTLEL and NP458-466 FQGRGVFEL) capable of activating cytotoxic T-cells in vitro were identified. 2. When the H5N1 nucleoprotein epitopes (NP373-381AMDSNTLEL and NP458-466FQGRGVFEL) were used with the single chain trimer system, they elicited effective cytotoxic T-cell responses against the corresponding nucleoprotein peptide-loaded cells in an HHD transgenic mouse model.
Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, China.
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YK
Cheung
S C S
SC
Cheng
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Y
Ke
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Y
Xie
D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism*; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D018984:Epitopes, T-Lymphocyte; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology*; D007252:Influenza Vaccines / Q000494:pharmacology*; D007251:Influenza, Human / Q000276:immunology*; D007371:Interferon-gamma / Q000378:metabolism; D051379:Mice; D009698:Nucleoproteins / Q000276:immunology*; D009976:Orthomyxoviridae Infections / Q000276:immunology
D018984:Epitopes, T-Lymphocyte; D007252:Influenza Vaccines; D009698:Nucleoproteins; D007371:Interferon-gamma