Immunophenotypic alterations in resident immune cells and myocardial fibrosis in the aging rhesus macaque (Macaca mulatta) heart.
The rhesus macaque (Macaca mulatta) is used extensively in translational biomedical research and drug development studies and is an important model of aging. Macaques often develop myocardial fibrosis with age, which can result in the loss of normal cardiac architecture with the expansion of the extracellular matrix and deposition of collagen. The etiology and pathogenesis of this pernicious process is poorly understood. Cardiac fibrosis was assessed using histologic and immunohistochemical techniques in cardiac tissue sections from 34 rhesus macaques. Overall left ventricular and left ventricular mid-myocardial interstitial/perivascular fibrosis were positively correlated with age (r = .6522, p < .0001 and r = .4704, p = .005, respectively). When divided into young (mean = 2.8 years), middle-aged (mean = 17.5 years), and advanced age (mean = 29.2 years) groups, immunophenotypic characterization of antigen presenting cells revealed differential expression of CD163 and DC-SIGN between the young and middle-aged groups compared to the advanced age group (p < .0001). HAM-56 expression decreased significantly in the advanced age cohort (p = .0021). The expression of CD8, CD163, and DC-SIGN correlated positively with age (r = .3999, p = .0191; r = .5676, p = .0005; r = .5245, p = .0014, respectively). These results show the importance of myocardial fibrosis as a common age-related pathology and additionally, alterations in T cell, macrophage, and dendritic cell phenotype in rhesus macaque myocardium are associated with age but unassociated with the fibrosis.
Division of Comparative Pathology, New England Primate Research Center, Harvard Medical School, Southborough, Massachuesettes 01772, USA.
Sheila C
SC
Macri
Charles C
CC
Bailey
Nicole Monts
NM
de Oca
Nilsa A
NA
Silva
Douglas L
DL
Rosene
Keith G
KG
Mansfield
Andrew D
AD
Miller
D000375:Aging / Q000276:immunology*; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D003713:Dendritic Cells / Q000378:metabolism; D005355:Fibrosis / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D007150:Immunohistochemistry; D016130:Immunophenotyping; D008253:Macaca mulatta; D008264:Macrophages / Q000378:metabolism*; D009206:Myocardium / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D018709:Statistics, Nonparametric; D013601:T-Lymphocytes / Q000378:metabolism*
D015703:Antigens, CD