Modeling of fungal biofilms using a rat central vein catheter.
Candida frequently grows as a biofilm, or an adherent community of cells protected from both the host immune system and antimicrobial therapies. Biofilms represent the predominant mode of growth for many clinical infections, including those associated with placement of a medical device. Here, we describe a model for Candida biofilm infection of one important clinical niche, a venous catheter. This animal model system incorporates the anatomical site, immune components, and fluid dynamics of a patient venous catheter infection and can be used for study of biofilm formation, drug resistance, and gene expression.
Division of Infectious Diseases, Department of Medicine and Medical Microbiology and Immunology, University of Wisconsin, Madison, WI, USA.
Jeniel E
JE
Nett
Karen
K
Marchillo
David R
DR
Andes
D000818:Animals; D018441:Biofilms; D002175:Candida / Q000254:growth & development* / Q000276:immunology / Q000302:isolation & purification; D002177:Candidiasis / Q000276:immunology / Q000382:microbiology* / Q000473:pathology; D002405:Catheterization, Central Venous; D004866:Equipment Contamination; D008297:Male; D023421:Models, Animal; D008954:Models, Biological; D051381:Rats; D017207:Rats, Sprague-Dawley