Cytoprotective activity of a trans-chalcone against hydrogen peroxide induced toxicity in hepatocellular carcinoma (HepG2) cells.
Dietary flavonoids have attracted attention as chemopreventive agents. Chalcones are abundantly present in nature starting from ferns to higher plants. Chemically 1,3-diphenyl-2-propen-1-ones, these are often cytotoxic in vitro. The cellular defense system (including glutathione, glutathione-related enzymes, and antioxidant and redox enzymes) plays a crucial role in cell survival and growth in aerobic organisms. In the present study, we aimed to evaluate the modulatory effect of trans-chalcone on protection from oxidative stress caused by hydrogen peroxide (H2O2) in hepatocellular carcinoma (HepG2) cells. Cell growth was evaluated by the 3-(4,5-dimethyl thiazol-2- yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Sub-toxic concentrations of compound (20 uM) increased cell survival and a decreased lipid peroxidation. The drug also decreased the H2O2 induction of glutathione related enzymes. Our results support the efficacy of trans-chalcone in offering protection against oxidative stress.
Department of Biotechnology, F/o Science, Hamdard University, New Delhi, India. sikanderbiochem@gmail.com
Mohammad
M
Sikander
Shabnam
S
Malik
Deepak
D
Yadav
Sahabuddin
S
Biswas
Deepshikha Pande
DP
Katare
Swatantar Kumar
SK
Jain
D000975:Antioxidants / Q000494:pharmacology; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D049109:Cell Proliferation; D002599:Chalcone / Q000031:analogs & derivatives* / Q000494:pharmacology*; D019610:Cytoprotection / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects; D008113:Liver Neoplasms / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*
D000975:Antioxidants; D002599:Chalcone; D006861:Hydrogen Peroxide; D005979:Glutathione Peroxidase; D005982:Glutathione Transferase; D005978:Glutathione