Novel monoclonal antibodies for identification of multicentric Castleman's disease; Kaposi's sarcoma-associated herpesvirus-encoded vMIP-I and vMIP-II.
Recent studies have indicated that vMIP-I and vMIP-II play important roles in the pathogenesis of Kaposi's sarcoma-associated herpesvirus (KSHV)-related diseases due to the effects of these proteins on vascularization. We developed monoclonal antibodies against KSHV-encoded viral macrophage inflammatory protein-I (vMIP-I) and vMIP-II to study these expression profiles and reveal the pathogenesis of KSHV-related diseases. The MAbs against vMIP-I and vMIP-II reacted to KSHV-infected cell lines after lytic induction. Both vMIP-I and the vMIP-II gene products were detected 24 h post-induction with 12-O-tetradecanoylphorbol-13-acetate until 60 h in the cytoplasm of primary effusion lymphoma cell lines. In clinical specimens, both vMIP-I and vMIP-II gene products were detected in the tissues of patients with multicentric Castleman's disease. On the other hand, only vMIP-II was detected in a subset of Kaposi's sarcoma. We concluded that these antibodies might be powerful tools to elucidate the pathogenesis of KSHV-related diseases.
Division of Virology, Department of Microbiology and Immunology, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan.
Kazushi
K
Nakano
Harutaka
H
Katano
Kenjiro
K
Tadagaki
Yuko
Y
Sato
Eriko
E
Ohsaki
Yasuko
Y
Mori
Koichi
K
Yamanishi
Keiji
K
Ueda
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D005871:Castleman Disease / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D002460:Cell Line; D019288:Herpesvirus 8, Human / Q000276:immunology / Q000302:isolation & purification / Q000502:physiology*; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D012514:Sarcoma, Kaposi / Q000175:diagnosis / Q000276:immunology / Q000821:virology*; D014764:Viral Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D014764:Viral Proteins; C108459:vMIP-1 protein, Human herpesvirus 8; C404961:vMIP-III protein, Herpesvirus 8, Human