Aldehyde dehydrogenase activity in cancer stem cells from canine mammary carcinoma cell lines.
Increasing evidence suggests that diverse solid tumours arise from a small population of cells known as cancer stem cells or tumour-initiating cells. Cancer stem cells in several solid tumours are enriched for aldehyde dehydrogenase (ALDH) activity. High levels of ALDH activity (ALDH(high)) were detected in four cell lines derived from canine mammary carcinomas. ALDH(high) cells were enriched in a CD44(+)CD24(-) population having self-renewal capacity. Xenotransplantation into immunodeficient mice demonstrated that 1×10(4) ALDH(high) cells were sufficient for tumour formation in all injected mice, whereas 1×10(4) ALDH(low) cells failed to initiate any tumours. ALDH(high)-derived tumours contained both ALDH(+) and ALDH(-) cells, indicating that these cells had cancer stem cell-like properties.
Department of Veterinary Pathology, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino, Tokyo 180-8602, Japan.
M
M
Michishita
R
R
Akiyoshi
H
H
Suemizu
T
T
Nakagawa
N
N
Sasaki
H
H
Takemitsu
T
T
Arai
K
K
Takahashi
D000444:Aldehyde Dehydrogenase / Q000378:metabolism*; D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism*; D051927:CD24 Antigen / Q000378:metabolism; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D004285:Dogs; D005260:Female; D005434:Flow Cytometry; D018960:Hyaluronan Receptors / Q000378:metabolism; D015674:Mammary Neoplasms, Animal / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D016688:Mice, Inbred NOD; D009368:Neoplasm Transplantation; D014411:Neoplastic Stem Cells / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology; D060888:Real-Time Polymerase Chain Reaction; D018874:Spheroids, Cellular / Q000378:metabolism* / Q000473:pathology / Q000637:transplantation; D014183:Transplantation, Heterologous
D014408:Biomarkers, Tumor; D051927:CD24 Antigen; D018960:Hyaluronan Receptors; D000444:Aldehyde Dehydrogenase