Macrophages actively accumulate malonyldialdehyde-modified but not enzymatically oxidized low density lipoprotein.
In this study, we show that low density lipoproteins (LDL) from human blood plasma which was oxidized by animal C-15 lipoxygenase is taken up by cultivated human macrophages with the same effectiveness as with non-oxidized (native) LDL. At the same time malonyldialdehyde-modified LDL is captured by cultivated macrophages very actively. Based on differences in catabolism of LDL with various levels of primary and secondary products of free-radical oxidation, it was offered to discriminate between the oxidized LDL itself (lipohydroperoxide-rich LDL) and the LDL that was chemically modified by free-radical oxidation secondary products of aldehyde nature. In this respect, aldehyde-modified but not oxidized (lipohydroperoxide-containing) LDL is atherogenic.
Russian Cardiology Research Complex, 3rd Cherepkovskaya street, 15a, Moscow, Russia 121552. lankin@cardio.ru
V Z
VZ
Lankin
A K
AK
Tikhaze
E M
EM
Kumskova
D000818:Animals; D050197:Atherosclerosis / Q000378:metabolism; D002478:Cells, Cultured; D006801:Humans; D015227:Lipid Peroxidation; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008084:Lipoxygenase / Q000737:chemistry*; D008264:Macrophages / Q000378:metabolism*; D008315:Malondialdehyde / Q000378:metabolism*; D011817:Rabbits
D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; D008315:Malondialdehyde; D008084:Lipoxygenase