Fabrication of cross-linked alginate beads using electrospraying for adenovirus delivery.
Cross-linked alginate beads containing adenovirus (Ad) were successfully fabricated using an electrospraying method to achieve the protection and release of Ad in a controlled manner. An aqueous alginate solution containing Ad was electrosprayed into an aqueous phase containing a cross-linking agent (calcium chloride) at different process variables (voltages, alginate concentrations, and flow rates). Alginate beads containing Ad were used for transduction of U343 glioma cells and the transduction efficiency of the alginate beads was measured by quantification of gene expression using a fluorescence-activated cell sorter at different time points. In vitro results of gene expression revealed that the Ad encapsulated in the alginate beads with 0.5 wt% of alginate concentration exhibited a high activity for a long period (over 7 days) and was released in a sustained manner from the alginate beads. The Ad-encapsulating alginate beads could be promising materials for local delivery of Ad at a high concentration into target sites.
Department of Chemical and Biomolecular Engineering, Yonsei University, 134 Shinchon-dong, Seodaemun-gu, Seoul 120-749, Republic of Korea.
Hongkwan
H
Park
Pyung-Hwan
PH
Kim
Taewon
T
Hwang
Oh-Joon
OJ
Kwon
Tae-Joon
TJ
Park
Sung-Wook
SW
Choi
Chae-Ok
CO
Yun
Jung Hyun
JH
Kim
D000256:Adenoviridae / Q000235:genetics*; D000464:Alginates / Q000737:chemistry*; D002122:Calcium Chloride; D045744:Cell Line, Tumor; D003432:Cross-Linking Reagents / Q000737:chemistry*; D003692:Delayed-Action Preparations; D004339:Drug Compounding; D004563:Electrochemistry; D005079:Excipients; D005434:Flow Cytometry; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000008:administration & dosage*; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000235:genetics; D006801:Humans; D009369:Neoplasms / Q000628:therapy; D010316:Particle Size; D012996:Solutions; D014161:Transduction, Genetic
D000464:Alginates; D003432:Cross-Linking Reagents; D003692:Delayed-Action Preparations; D005079:Excipients; D012996:Solutions; D049452:Green Fluorescent Proteins; D002122:Calcium Chloride