CRM1-dependent trafficking of retroviral Gag proteins revisited.
We analyzed the nuclear trafficking ability of Gag proteins from six retroviral genera. Contrary to a previous report, human immunodeficiency virus type 1 (HIV-1) Gag showed no propensity to cycle through the nucleus. The only Gag protein that displayed CRM1-dependent nuclear cycling was that of Rous sarcoma virus (RSV). Surprisingly, this cycling could be eliminated without compromising infectivity by replacing the RSV Gag N-terminal matrix (MA) domain with HIV MA.
New York Medical College, Valhalla, New York, USA.
Mariju F
MF
Baluyot
Sarah A
SA
Grosse
Terri D
TD
Lyddon
Sanath K
SK
Janaka
Marc C
MC
Johnson
D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D023061:Gene Order; D015683:Gene Products, gag / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D028884:Karyopherins / Q000378:metabolism*; D021381:Protein Transport; D011533:Proviruses / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism*; D012190:Retroviridae / Q000235:genetics / Q000378:metabolism*; D000097606:Exportin 1 Protein
D015683:Gene Products, gag; D028884:Karyopherins; D018160:Receptors, Cytoplasmic and Nuclear; D000097606:Exportin 1 Protein