Integrated multimodal endomicroscopy platform for simultaneous en face optical coherence and two-photon fluorescence imaging.
We report an all-fiber-optic scanning, multimodal endomicroscope capable of simultaneous optical coherence tomography (OCT) and two-photon fluorescence (TPF) imaging. Both imaging modalities share the same miniature fiber-optic scanning endomicroscope, which consists of a double-clad fiber with a core operating in single mode at both the OCT (1310 nm) and two-photon excitation (1550 nm) wavelengths, a piezoelectric two-dimensional fiber-optic beam scanner, and a miniature aspherical compound lens suitable for simultaneous acquisition of en face OCT and TPF images. A fiber-optic wavelength division multiplexer was employed in the integrated platform to combine the low coherence OCT light source and the femtosecond two-photon excitation laser into the same optical path. Preliminary imaging results of cell cultures and mouse tissue ex vivo demonstrate the feasibility of simultaneous real-time OCT and TPF imaging in a scanning endomicroscopy setting for the first time.
Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland 21210, USA.
Jiefeng
J
Xi
Yongping
Y
Chen
Yuying
Y
Zhang
Kartikeya
K
Murari
Ming-Jun
MJ
Li
Xingde
X
Li
D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D045744:Cell Line, Tumor; D006801:Humans; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods*; D017785:Photons; D013997:Time Factors; D041623:Tomography, Optical Coherence / Q000379:methods*