Analysis of cancer cell lipids using matrix-assisted laser desorption/ionization 15-T Fourier transform ion cyclotron resonance mass spectrometry.
A combination of methodologies using the extremely high mass accuracy and resolution of 15-T Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) was introduced for the identification of intact cancer cell phospholipids. Lipids from a malignant glioma cell line were initially analyzed at a resolution of >200,000 and identified by setting the mass tolerance to ±1 mDa using matrix-assisted laser desorption/ionization (MALDI) 15-T FT-ICR MS in positive ion mode. In most cases, a database search of potential lipid candidates using the exact masses of the lipids yielded only one possible chemical composition. Extremely high mass accuracy (<0.1 ppm) was then attained by using previously identified lipids as internal standards. This, combined with an extremely high resolution (>800,000), yielded well-resolved isotopic fine structures allowing for the identification of lipids by MALDI 15-T FT-ICR MS without using tandem mass spectrometric (MS/MS) analysis. Using this method, a total of 38 unique lipids were successfully identified.
Department of Chemistry, Chungnam National University, Daejeon, Republic of Korea.
Hyo-Jik
HJ
Yang
Kyu Hwan
KH
Park
Dong Wan
DW
Lim
Hyun Sik
HS
Kim
Jeongkwon
J
Kim
D045744:Cell Line, Tumor; D017357:Cyclotrons; D016208:Databases, Factual; D005583:Fourier Analysis; D006801:Humans; D008055:Lipids / Q000032:analysis*; D009369:Neoplasms / Q000737:chemistry*; D010743:Phospholipids / Q000032:analysis; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D008055:Lipids; D010743:Phospholipids