Environmental contamination with extended-spectrum β-lactamases: is there any difference between Escherichia coli and Klebsiella spp?
BACKGROUND
The hospital environment contributes to the spread of extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae (ESBL-PE) during outbreaks. We aimed to assess the rate of environmental contamination in rooms occupied by ESBL carriers or infected children and to identify risk factors associated with contamination.


METHODS
Five environmental surface samples were systematically performed in rooms occupied by ESBL-PE carrier or infected children.


RESULTS
Forty-six Escherichia coli and 48 Klebsiella infected/carrier patients were included in the study. Nineteen (4%) of the 470 environmental samples performed yielded ESBL-PE. Klebsiella spp was the most frequent species isolated (16, 89%), whereas E coli and Citrobacter freundii were reported twice and once, respectively. Ten of the 19 (52%) isolates were identical to the corresponding strains isolated from children. Multivariate analysis highlighted ESBL-producing Klebsiella carriage/infection as the only risk factor significantly associated with surface contamination (P = .024).


CONCLUSIONS
Our data suggest that hospital environmental contamination is more frequent in instances of fecal carriage or infection with ESBL-producing Klebsiella than ESBL-producing E coli. Reinforcing hygiene measures around ESBL-producing Klebsiella might be necessary to reduce the spread of ESBL-PE in hospital environments.
Université Paris Descartes, Paris, France.
Helene
H
Guet-Revillet
Alban
A
Le Monnier
Nelly
N
Breton
Philippe
P
Descamps
Herve
H
Lecuyer
Imane
I
Alaabouche
Constance
C
Bureau
Xavier
X
Nassif
Jean-Ralph
JR
Zahar
D002353:Carrier State / Q000382:microbiology; D002648:Child; D002675:Child, Preschool; D016971:Citrobacter freundii / Q000201:enzymology / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000382:microbiology*; D004783:Environmental Microbiology; D004926:Escherichia coli / Q000201:enzymology* / Q000302:isolation & purification*; D006801:Humans; D007709:Klebsiella / Q000201:enzymology* / Q000302:isolation & purification*; D010362:Patients' Rooms; D011446:Prospective Studies; D001618:beta-Lactamases / Q000378:metabolism*
D001618:beta-Lactamases