Fluid biopsy in patients with metastatic prostate, pancreatic and breast cancers.
Hematologic spread of carcinoma results in incurable metastasis; yet, the basic characteristics and travel mechanisms of cancer cells in the bloodstream are unknown. We have established a fluid phase biopsy approach that identifies circulating tumor cells (CTCs) without using surface protein-based enrichment and presents them in sufficiently high definition (HD) to satisfy diagnostic pathology image quality requirements. This 'HD-CTC' assay finds >5 HD-CTCs mL(-1) of blood in 80% of patients with metastatic prostate cancer (n = 20), in 70% of patients with metastatic breast cancer (n = 30), in 50% of patients with metastatic pancreatic cancer (n = 18), and in 0% of normal controls (n = 15). Additionally, it finds HD-CTC clusters ranging from 2 HD-CTCs to greater than 30 HD-CTCs in the majority of these cancer patients. This initial validation of an enrichment-free assay demonstrates our ability to identify significant numbers of HD-CTCs in a majority of patients with prostate, breast and pancreatic cancers.
Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Dena
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Marrinucci
Kelly
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Bethel
Anand
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Kolatkar
Madelyn S
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Luttgen
Michael
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Malchiodi
Franziska
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Baehring
Katharina
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Voigt
Daniel
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Lazar
Jorge
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Nieva
Lyudmila
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Bazhenova
Andrew H
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Ko
W Michael
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Korn
Ethan
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Schram
Michael
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Coward
Xing
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Yang
Thomas
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Metzner
Rachelle
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Lamy
Meghana
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Honnatti
Craig
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Yoshioka
Joshua
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Kunken
Yelena
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Petrova
Devin
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Sok
David
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Nelson
Peter
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Kuhn
D000328:Adult; D001706:Biopsy / Q000379:methods*; D001943:Breast Neoplasms / Q000473:pathology*; D005260:Female; D006801:Humans; D007090:Image Interpretation, Computer-Assisted; D007633:Keratins / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009360:Neoplastic Cells, Circulating / Q000473:pathology*; D010190:Pancreatic Neoplasms / Q000473:pathology*; D011471:Prostatic Neoplasms / Q000473:pathology*; D012680:Sensitivity and Specificity; D055815:Young Adult
D007633:Keratins