Thin-layer hydroxyapatite deposition on a nanofiber surface stimulates mesenchymal stem cell proliferation and their differentiation into osteoblasts.
Pulsed laser deposition was proved as a suitable method for hydroxyapatite (HA) coating of coaxial poly-ɛ-caprolactone/polyvinylalcohol (PCL/PVA) nanofibers. The fibrous morphology of PCL/PVA nanofibers was preserved, if the nanofiber scaffold was coated with thin layers of HA (200 nm and 400 nm). Increasing thickness of HA, however, resulted in a gradual loss of fibrous character. In addition, biomechanical properties were improved after HA deposition on PCL/PVA nanofibers as the value of Young's moduli of elasticity significantly increased. Clearly, thin-layer hydroxyapatite deposition on a nanofiber surface stimulated mesenchymal stem cell viability and their differentiation into osteoblasts. The optimal depth of HA was 800 nm.
Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Science of the Czech Republic, Videnska 1083, 14240 Prague, Czech Republic.
Eva
E
Prosecká
Matej
M
Buzgo
Michala
M
Rampichová
Tomáš
T
Kocourek
Petra
P
Kochová
Lucie
L
Vysloužilová
Daniel
D
Tvrdík
Miroslav
M
Jelínek
David
D
Lukáš
Evžen
E
Amler
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D017886:Durapatite / Q000737:chemistry / Q000494:pharmacology; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D057139:Nanofibers / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology*; D011091:Polyesters / Q000737:chemistry; D011142:Polyvinyl Alcohol / Q000737:chemistry; D013552:Swine
D011091:Polyesters; C016240:polycaprolactone; D011142:Polyvinyl Alcohol; D017886:Durapatite