Environmentally sensitive fluorescent sensors based on synthetic peptides.
Biosensors allow the direct detection of molecular analytes, by associating a biological receptor with a transducer able to convert the analyte-receptor recognition event into a measurable signal. We review recent work aimed at developing synthetic fluorescent molecular sensors for a variety of analytes, based on peptidic receptors labeled with environmentally sensitive fluorophores. Fluorescent indicators based on synthetic peptides are highly interesting alternatives to protein-based sensors, since they can be synthesized chemically, are stable, and can be easily modified in a site-specific manner for fluorophore coupling and for immobilization on solid supports.
Biosensor group, CNRS/Université de Strasbourg, IREBS, Parc d'Innovation, Boulevard Sébastien Brandt, BP 10413, 67412 Illkirch cedex, France. laurence.choulier@unistra.fr
Laurence
L
Choulier
Karin
K
Enander
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D004867:Equipment Design; D005453:Fluorescence; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry; D014159:Transducers
D005456:Fluorescent Dyes; D010455:Peptides; D011506:Proteins