Effect of laser phototherapy on the hyalinization following orthodontic tooth movement in rats.
OBJECTIVE
We aimed to assess histologic changes after the use of laser phototherapy (LPT) during induced tooth movement with 40 g/F on young adult male rats.


BACKGROUND
Hyalinization is a sterile necrosis at the pressure zone of the periodontal ligament observed during the initial stages of the orthodontic movement, and extensive hyaline areas might cause an important delay in the tooth movement. The use of LPT is considered an enhancement factor for bone repair, as it stimulates microcirculation as well as the cellular metabolism.


METHODS
Thirty animals were divided into two groups (n=15), named according to the time of animal death (7, 13, and 19 days). Half of the animals in each group were subjected to irradiation with infrared (IR) laser (λ790 nm, round shaped beam, 40 mW, continuous wave (CW), diameter=2 mm (0.0314 cm(2)), 1.273 W/cm(2), time=2×112 sec+1×275 sec (total time 499 sec), 2×142.6/4.48 J+1×350/11 J, 635.2 J/cm(2)/20 J/ session), during orthodontic movement, the other half were used as nonirradiated controls. After animal death, specimens were sectioned, processed, and stained with hematoxylin and eosin (HE) and Sirius Red, and were used for semi-quantitative histologic analysis by light microscopy. Data were statistically analyzed.


RESULTS
We demonstrated that LPT positively affected an important aspect of dental movement; the hyalinization. In the present study, we found a significant reduced expression of hyalinization after 19 days. On irradiated subjects, hyalinization was increased at day 7 with significant reduction at day 13.


CONCLUSIONS
It is possible to conclude that the use of laser light caused histologic alterations during the orthodontic movement characterized by increased formation of areas of hyalinization at early stages, and late reduction when compared to nonirradiated animals.
Centro de Ortodontia e Ortopedia Facial Prof. José Édimo Soares Martins, School of Dentistry, Federal University of Bahia, Salvador, BA, Brazil.
Fernando A L
FA
Habib
Sabrina K C
SK
Gama
Luciana M P
LM
Ramalho
Maria Cristina T
MC
Cangussú
Fernando P
FP
dos Santos Neto
Juliana A
JA
Lacerda
Telma M
TM
de Araújo
Antônio L B
AL
Pinheiro
D000539:Alveolar Process / Q000473:pathology / Q000528:radiation effects*; D000818:Animals; D004195:Disease Models, Animal; D006818:Hyalin / Q000378:metabolism / Q000528:radiation effects*; D007150:Immunohistochemistry; D028022:Low-Level Light Therapy / Q000379:methods*; D008297:Male; D010513:Periodontal Ligament / Q000473:pathology / Q000528:radiation effects*; D010784:Photomicrography; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D014087:Tooth Movement Techniques / Q000379:methods*; D016896:Treatment Outcome