Changes in RACK1 expression induce defects in nodulation and development in Phaseolus vulgaris.
RACK1 is a scaffold protein with the ability to interact in a regulated manner with a diverse number of ligands from distinct signal-transduction pathways. This assessment allowed us to infer that it may be involved in different processes such as nodulation. In a recent study we showed by silencing, that PvRACK1 has a pivotal role in cell expansion and in symbiosome and bacteroid integrity during nodule development in Phaseolus vulgaris. On the other hand, we have also observed that its over-expression provokes a dramatic phenotype in: (a) seedlings that have been exposed to heat, in which systemic necrosis is induced; and (b) in Agrobacterium rhizogenes-transformed roots, where nodulation is strongly inhibited and nodules show early senescent symptoms. These findings indicate that PvRACK1 may be an integrator of diverse signal-transduction pathways in processes as varied as nodulation, cell expansion, heat stress responses, and systemic activation of necrosis.
Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, UNAM, Morelos, Mexico.
Tania
T
Islas-Flores
Gabriel
G
Guillén
Federico
F
Sánchez
Marco A
MA
Villanueva
D008854:Microscopy, Electron; D009586:Nitrogen Fixation / Q000502:physiology*; D010455:Peptides / Q000378:metabolism* / Q000502:physiology; D027805:Phaseolus / Q000254:growth & development* / Q000502:physiology; D000075370:Receptors for Activated C Kinase
D010455:Peptides; D000075370:Receptors for Activated C Kinase; C070301:peptide I