Annotation of a serum N-glycan library for rapid identification of structures.
Glycosylation is one of the most common post-translational modifications of proteins and has been shown to change with various pathological states including cancer. Global glycan profiling of human serum based on mass spectrometry has already led to several promising markers for diseases. The changes in glycan structure can result in altered monosaccharide composition as well as in the linkages between the monosaccharides. High-throughput glycan structural elucidation is not possible because of the lack of a glycan template to expedite identification. In an effort toward rapid profiling and identification of glycans, we have constructed a library of structures for the serum glycome to aid in the rapid identification of serum glycans. N-Glycans from human serum glycoproteins are used as a standard and compiled into a library with exact structure (composition and linkage), liquid chromatography retention time, and accurate mass. Development of the library relies on highly reproducible nanoLC-MS retention times. Tandem MS and exoglycosidase digestions were used for structural elucidation. The library currently contains over 300 entries with 50 structures completely elucidated and over 60 partially elucidated structures. This database is steadily growing and will be used to rapidly identify glycans in unknown biological samples.
Department of Chemistry, University of California , Davis, California 95616, United States.
Danielle
D
Aldredge
Hyun Joo
HJ
An
Ning
N
Tang
Keith
K
Waddell
Carlito B
CB
Lebrilla
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid / Q000592:standards; D030562:Databases, Protein; D006023:Glycoproteins / Q000097:blood* / Q000737:chemistry; D006026:Glycoside Hydrolases / Q000737:chemistry; D006031:Glycosylation; D006801:Humans; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000097:blood* / Q000737:chemistry; D020033:Protein Isoforms / Q000097:blood / Q000737:chemistry; D011499:Protein Processing, Post-Translational; D012015:Reference Standards; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000592:standards; D053719:Tandem Mass Spectrometry / Q000592:standards
D006023:Glycoproteins; D011134:Polysaccharides; D020033:Protein Isoforms; D006026:Glycoside Hydrolases