Acceleration of tendon-bone healing in anterior cruciate ligament reconstruction using an enamel matrix derivative in a rat model.
We examined whether enamel matrix derivative (EMD) could improve healing of the tendon-bone interface following reconstruction of the anterior cruciate ligament (ACL) using a hamstring tendon in a rat model. ACL reconstruction was performed in both knees of 30 Sprague-Dawley rats using the flexor digitorum tendon. The effect of commercially available EMD (EMDOGAIN), a preparation of matrix proteins from developing porcine teeth, was evaluated. In the left knee joint the space around the tendon-bone interface was filled with 40 µl of EMD mixed with propylene glycol alginate (PGA). In the right knee joint PGA alone was used. The ligament reconstructions were evaluated histologically and biomechanically at four, eight and 12 weeks (n = 5 at each time point). At eight weeks, EMD had induced a significant increase in collagen fibres connecting to bone at the tendon-bone interface (p = 0.047), whereas the control group had few fibres and the tendon-bone interface was composed of cellular and vascular fibrous tissues. At both eight and 12 weeks, the mean load to failure in the treated specimens was higher than in the controls (p = 0.009). EMD improved histological tendon-bone healing at eight weeks and biomechanical healing at both eight and 12 weeks. EMD might therefore have a human application to enhance tendon-bone repair in ACL reconstruction.
Hiroshima University, Kasumi 1-2-3, Minami-ku, Hiroshima-City 734-8551, Japan.
Y
Y
Kadonishi
M
M
Deie
T
T
Takata
M
M
Ochi
D000818:Animals; D000070598:Anterior Cruciate Ligament Injuries; D059549:Anterior Cruciate Ligament Reconstruction / Q000379:methods*; D003094:Collagen / Q000378:metabolism; D003746:Dental Enamel Proteins / Q000494:pharmacology*; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005260:Female; D011184:Postoperative Period; D051381:Rats; D017207:Rats, Sprague-Dawley; D013314:Stress, Mechanical; D013710:Tendons / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology / Q000637:transplantation*; D013977:Tibia / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D014945:Wound Healing / Q000187:drug effects*
D003746:Dental Enamel Proteins; C109069:enamel matrix proteins; D003094:Collagen