Determination of 5-hydroxymethyl-2-furfural, albiflorin, paeoniflorin, liquiritin, ferulic acid, nodakenin, and glycyrrhizin by HPLC-PDA, and evaluation of the cytotoxicity of Palmul-tang, a traditional Korean herbal medicine.
A high-performance liquid chromatographic (HPLC) method was developed for quantitative analysis of seven components, 5-hydroxymethyl-2-furaldehyde (1), albiflorin (2), paeoniflorin (3), liquiritin (4), ferulic acid (5), nodakenin (6), and glycyrrhizin (7) of Palmul-tang (PMT), a traditional Korean medicine. HPLC analysis was performed using a Gemini C18 column at 40°C, and photodiode array (PDA) detection at 230 nm, 254 nm, 280 nm, 320 nm, and 330 nm was used for quantification of the seven components in PMT. The mobile phase was a gradient flow composed of two solvent systems. Solvent A was 1.0% (v/v) aqueous acetic acid and solvent B was acetonitrile containing 1.0% (v/v) acetic acid. Calibration curves were acquired with r                         (2) values > 0.9998, and the relative standard deviations (RSDs, %) for intra- and interday precision were both less than 6.0%. The recovery of each component was in the range of 90.66-103.79%, with a RSD less than 5.0%. The contents of the seven components in PMT range form 0.61-6.21 mg/g. Additionally, we investigated the cytotoxicity of the extract against the RBL-1 and BEAS-2B cell lines, as well as splenocytes.
Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine, Daejeon 305-811, Korea.
Chang-Seob
CS
Seo
Mee-Young
MY
Lee
Hye-Sun
HS
Lim
Su-Jeong
SJ
Kim
Hyekyung
H
Ha
Jin-Ah
JA
Lee
Hyeun-Kyoo
HK
Shin
D000818:Animals; D001565:Benzoates / Q000032:analysis / Q000737:chemistry; D001952:Bridged-Ring Compounds / Q000032:analysis / Q000737:chemistry; D045744:Cell Line, Tumor; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003373:Coumaric Acids / Q000032:analysis / Q000737:chemistry; D003374:Coumarins / Q000032:analysis / Q000737:chemistry; D003603:Cytotoxins / Q000032:analysis* / Q000737:chemistry*; D004353:Drug Evaluation, Preclinical; D044950:Flavanones / Q000032:analysis / Q000737:chemistry; D005662:Furaldehyde / Q000031:analogs & derivatives / Q000032:analysis / Q000737:chemistry; D005960:Glucosides / Q000032:analysis / Q000737:chemistry; D019695:Glycyrrhizic Acid / Q000032:analysis / Q000737:chemistry; D055670:Medicine, Korean Traditional; D051379:Mice; D008807:Mice, Inbred BALB C; D039821:Monoterpenes; D028321:Plant Preparations / Q000032:analysis* / Q000737:chemistry*; D051381:Rats; D006027:Glycosides
D001565:Benzoates; D001952:Bridged-Ring Compounds; D003373:Coumaric Acids; D003374:Coumarins; D003603:Cytotoxins; D044950:Flavanones; D005662:Furaldehyde; D005960:Glucosides; D019695:Glycyrrhizic Acid; D039821:Monoterpenes; D028321:Plant Preparations; C015423:peoniflorin; D006027:Glycosides; C014959:albiflorin; C008046:5-hydroxymethylfurfural; C004999:ferulic acid; C471579:nodakenin; C512196:liquiritin