Sol-gel method to fabricate CaP scaffolds by robocasting for tissue engineering.
Highly porous calcium phosphate (CaP) scaffolds for bone-tissue engineering were fabricated by combining a robocasting process with a sol-gel synthesis that mixed Calcium Nitrate Tetrahydrate and Triethyl Phosphite precursors in an aqueous medium. The resulting gels were used to print scaffolds by robocasting without the use of binder to increase the viscosity of the paste. X-ray diffraction analysis confirmed that the process yielded hydroxyapatite and β-tricalcium phosphate biphasic composite powders. Thus, the scaffold composition after crystallization of the amorphous structure could be easily modified by varying the initial Ca/P ratio during synthesis. The compressive strengths of the scaffolds are ~6 MPa, which is in the range of human cancellous bone (2-12 MPa). These highly porous scaffolds (~73 vol% porosity) are composed of macro-pores of ~260 μm in size; such porosity is expected to enable bone ingrowth into the scaffold for bone repair applications. The chemistry, porosity, and surface topography of such scaffolds can also be modified by the process parameters to favor bone formation. The studied sol-gel process can be used to coat these scaffolds by dip-coating, which induces a significant enhancement of mechanical properties. This can adjust scaffold properties such as composition and surface morphology, which consequently may improve their performances.
Materials Science Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. manuel.houmard@wanadoo.fr
Manuel
M
Houmard
Qiang
Q
Fu
Eduardo
E
Saiz
Antoni P
AP
Tomsia
D002130:Calcium Phosphates / Q000378:metabolism*; D005782:Gels; D006801:Humans; D008855:Microscopy, Electron, Scanning; D023822:Tissue Engineering; D014783:Viscosity; D014961:X-Ray Diffraction
D002130:Calcium Phosphates; D005782:Gels; C020243:calcium phosphate