[The electrophoretic cell analysis applying the instrument complex "Cito-expert"].
The electric discharge of living cells and Z-potential of cell membrane are among the most important indicators to determine at cell level the homeostasis of any living organism, including organs and tissues. The cell electrophoresis is considered as the most handy, accurate and reliable practical technique to determine these indicators. The authors developed the first national serial instrument complex for cell electrophoresis. The device differs from international models by using the alternating electric field and original electrophoretic camera. The instrument complex is protected by patents and permits to determine the cells' oscillation amplitude in alternating field proportional to their effective charge. The device also determines the portion of active cells and their distribution according amplitudes and the asymmetry and excess of this distribution. The duration of single analysis consists 5-8 min and the volume of cell suspension is 40 microL. The analysis is applied to 100-300 cells and the voltage is about 10-30 V on the camera electrodes. The instrument complex is most prospectively applied to diagnose and monitor the treatment of critical conditions with intoxication, to express-control the toxicity of various liquid mediums and biologic activity of nano-materials on cell level and to screening promptly the pharmacologic substances and pharmaceuticals. The published nowadays results of studies performed on different devices, permit to expand significantly the sphere of practical and scientific application of cell electrophoresis.
E P
EP
Sukhonenko
N B
NB
Lisitsin
V A
VA
Palagin
A A
AA
Solov'ev
N A
NA
Kir'ianov
D000328:Adult; D001773:Blood Cells / Q000737:chemistry / Q000502:physiology; D002462:Cell Membrane / Q000737:chemistry / Q000502:physiology*; D004586:Electrophoresis / Q000295:instrumentation* / Q000379:methods*; D006801:Humans; D007231:Infant, Newborn; D008564:Membrane Potentials; D008875:Middle Aged; D008991:Monitoring, Physiologic / Q000295:instrumentation* / Q000379:methods*; D049329:Nanostructures