[Effect and mechanism of Andrographitis Herba on human CD4+ T cell promoters CXCR4 and CCR5].
OBJECTIVE
Utilizing a gene reporter technique to study the effects of Andrographitis Herba on human CXCR4 and CCR5 promoters.


METHODS
Inhibition of CXCR4 and CCR5 on T cells of healthy volunteers was analyzed by RT PCR, Western blot and flow cytometry. The human CXCR4 and CCR5 promoters driving a luciferase reporter in vectors pGLA. 17-CXCR4 and pGLA. 17-CCR5 were transfected into H9 stem cells. G418 was used for selecting stable cell lines. Rat sera thus medicated was collected and added to the transfected H9 cells, in which the expression of CXCR4 and CCR5 promoters was detected.


RESULTS
They showed that the mRNA and protein expression levels of CXCR4 and CCR5 in human CD4+ T cells decreased significantly after taking Andrographitis Herba (P<0.05). Furthermore human CXCR4 and CCR5 promoter activity was downregulated significantly by sera from rats medicated with Andrographitis Herba.


CONCLUSIONS
Andrographitis Herba may have the effect of down-regulating CXCR4 and CCR5 promoters. It provides a feasible experimental platform for screening herbal medicine as the treatment of HIV/AIDS.
Department of Pathogenic Organism Biology, Henan University of Traditonal Chinese Medicine, Zhengzhou 450008, China.
Long
L
Feng
Guoqiang
G
Zhao
Yunyun
Y
Ma
Min
M
Li
Jing
J
Ma
Jing
J
Jin
Ying
Y
Cui
D000328:Adult; D035685:Andrographis / Q000737:chemistry*; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D002460:Cell Line; D015536:Down-Regulation / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D006801:Humans; D008297:Male; D011401:Promoter Regions, Genetic / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D019713:Receptors, CCR5 / Q000235:genetics* / Q000378:metabolism; D019718:Receptors, CXCR4 / Q000235:genetics* / Q000378:metabolism; D055815:Young Adult
D004365:Drugs, Chinese Herbal; D019713:Receptors, CCR5; D019718:Receptors, CXCR4