Combined RNAi and localization for functionally dissecting long noncoding RNAs.
Whereas methods to comprehensively study cellular roles of protein-coding genes are available, techniques to systematically investigate long noncoding RNAs (lncRNAs), which have been implicated in diverse biological pathways, are limited. Here we report combined knockdown and localization analysis of noncoding RNAs (c-KLAN) that merges functional characterization and localization approaches to study lncRNAs. Using this technique we identified transcripts that regulate mouse embryonic stem cell identity.
University of Technology Dresden, University Hospital and Medical Faculty Carl Gustav Carus, Department of Medical Systems Biology, Dresden, Germany.
Debojyoti
D
Chakraborty
Dennis
D
Kappei
Mirko
M
Theis
Anja
A
Nitzsche
Li
L
Ding
Maciej
M
Paszkowski-Rogacz
Vineeth
V
Surendranath
Nicolas
N
Berger
Herbert
H
Schulz
Kathrin
K
Saar
Norbert
N
Hubner
Frank
F
Buchholz
D000818:Animals; D053595:Embryonic Stem Cells / Q000378:metabolism; D055785:Gene Knockdown Techniques; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D034622:RNA Interference; D022661:RNA, Untranslated / Q000235:genetics* / Q000378:metabolism*
D022661:RNA, Untranslated