Characterization of European forage maize lines for stover composition and associations with polymorphisms within O-methyltransferase genes.
Cell wall components, such as lignin, cellulose, and hemicelluloses, play an important role in the conversion efficiency of corn stover into ethanol. Understanding the molecular basis of cell wall formation is fundamental for marker assisted selection to develop lines more suitable for ethanol production. In this study, we evaluated a set of 40 European forage maize lines for cellulose, lignin, total hemicellulose, glucuronoarabinoxylan (GAX), and monosaccharides, such as arabinose (ara), xylose (xyl), and glucuronic acid (GlcA). The most significant correlations were observed between hemicelluloses and GAX (0.9), and hemicelluloses and cellulose (-0.81). Cell wall digestibility (CWD, estimated by digestible neutral detergent fiber, DNDF) was negatively correlated with Xyl (-0.34). The association analysis between the evaluated traits and polymorphisms within ten "lignin" genes revealed significant associations between polymorphisms within CCoAOMT1, CCoAOMT2, 4CL2 and C4H, and cellulose/xyl, cellulose, cellulose, and GclA, respectively. None of the QTPs identified in this study corresponded to previously reported CWD QTPs.
Department of Agronomy, Iowa State University, Agronomy Hall, Ames, IA 50011, USA. evertonb@hotmail.com
Everton A
EA
Brenner
Andre M
AM
Salazar
Olga A
OA
Zabotina
Thomas
T
Lübberstedt
D001947:Breeding; D002473:Cell Wall / Q000378:metabolism; D002482:Cellulose / Q000032:analysis / Q000378:metabolism; D002874:Chromosome Mapping; D000431:Ethanol / Q000378:metabolism; D008031:Lignin / Q000032:analysis / Q000378:metabolism*; D015810:Linkage Disequilibrium; D008780:Methyltransferases / Q000235:genetics* / Q000378:metabolism; D009005:Monosaccharides / Q000032:analysis / Q000378:metabolism; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011110:Polymorphism, Genetic / Q000235:genetics*; D011134:Polysaccharides / Q000032:analysis / Q000378:metabolism*; D040641:Quantitative Trait Loci; D014990:Xylans / Q000032:analysis / Q000378:metabolism*; D003313:Zea mays / Q000235:genetics* / Q000378:metabolism
D009005:Monosaccharides; D010940:Plant Proteins; D011134:Polysaccharides; D014990:Xylans; C114470:glucuronoarabinoxylan; D000431:Ethanol; C007916:hemicellulose; D002482:Cellulose; D008031:Lignin; D008780:Methyltransferases