Protective effects of lotus (Nelumbo nucifera Gaertn) germ oil against carbon tetrachloride-induced injury in mice and cultured PC-12 cells.
The protective effects of lotus germ oil on liver and kidney damage by carbon tetrachloride-induced chronic hepatotoxicity in mice, PC-12 cells, and DNA damage were investigated. The mice were treated orally with lotus germ oil or dl-α-tocopherol after administration CCl(4) for 49 consecutive days. The levels of key antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT), and the concentration of glutathione (GSH), as well as the concentration of malondialdehyde (MDA), an indicator of lipid peroxidation, were determined in homogenates of the liver and the kidney. The pathological histology of the liver was also examined. The activities of SOD, CAT, and the concentration of GSH were increased significantly (p<0.05-0.01) after treated with lotus germ oil in a concentration-dependent manner. Whereas, the content of the peroxidation product MDA were decreased significantly (p<0.05), similar to the serum levels of hepatic enzyme biomarkers (alanine aminotransferase and aspartate aminotransferase). Furthermore, lotus germ oil could inhibit the conversion of super-coiled pBR322 plasmid DNA to the open circular form and apoptosis of hydrogen peroxide-induced PC-12 cells. The result of this study suggested that the lotus germ oil could be recognized as powerful "functional oil" against oxidative stress.
Department of Food Science and Technology, Ginling College, Nanjing Normal University, 122# Ninghai Road, Nanjing 210097, PR China.
Lishuang
L
Lv
Cunbo
C
Jiang
Jianlin
J
Li
Tiesong
T
Zheng
D000818:Animals; D002251:Carbon Tetrachloride / Q000633:toxicity*; D002374:Catalase / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000473:pathology / Q000503:physiopathology / Q000517:prevention & control*; D004249:DNA Damage; D005978:Glutathione / Q000378:metabolism; D008111:Liver Function Tests; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D031653:Nelumbo / Q000737:chemistry*; D016716:PC12 Cells; D010938:Plant Oils / Q000494:pharmacology*; D051381:Rats; D013482:Superoxide Dismutase / Q000378:metabolism
D010938:Plant Oils; D008315:Malondialdehyde; D002251:Carbon Tetrachloride; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione