Molecular characterisation of porcine miR-155 and its regulatory roles in the TLR3/TLR4 pathways.
MiR-155 plays very important roles in host inflammation and immunity. However, few studies have focused on miR-155 in livestock. In this study, the molecular characterisation of miR-155 and its functional roles in TLR3/TLR4 signalling pathways were investigated in pigs. The results indicated that miR-155 was highly expressed in the spleen and fat tissues of the pig. In PK-15 cells, miR-155 was up-regulated 4h after LPS stimulation and up-regulated 12h and 24h after poly (I:C) stimulation. Furthermore, the overexpression of miR-155 significantly activated the TLR3/TLR4 signalling pathways, and the inhibition of miR-155 suppressed these pathways. Thus, miR-155 played positive regulatory roles in TLR3/TLR4 signalling pathways. Additionally, one T/C SNP of miR-155 was significantly associated with basophil percentage (BA%), absolute eosinophili value (EO) and the distribution width of the least squares mean of CD3-CD4-CD8+ T cells (DWT) in pigs. Our study offers new evidence on the immune function of miR-155 in pigs.
Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education and Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China.
Congcong
C
Li
Huabin
H
He
Mengjin
M
Zhu
Shuhong
S
Zhao
Xinyun
X
Li
D000273:Adipose Tissue / Q000276:immunology; D000818:Animals; D002460:Cell Line; D005786:Gene Expression Regulation / Q000276:immunology; D007111:Immunity, Cellular / Q000235:genetics; D008070:Lipopolysaccharides / Q000276:immunology; D058751:Livestock; D035683:MicroRNAs / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D015394:Molecular Structure; D011070:Poly I-C / Q000276:immunology; D020641:Polymorphism, Single Nucleotide; D015398:Signal Transduction / Q000235:genetics / Q000276:immunology; D013154:Spleen / Q000276:immunology; D013552:Swine / Q000235:genetics / Q000276:immunology*; D051196:Toll-Like Receptor 3 / Q000276:immunology*; D051197:Toll-Like Receptor 4 / Q000276:immunology*; D019076:Transgenes / Q000235:genetics
D008070:Lipopolysaccharides; D035683:MicroRNAs; D051196:Toll-Like Receptor 3; D051197:Toll-Like Receptor 4; D011070:Poly I-C