Emergence of a mutL mutation causing multilocus sequence typing-pulsed-field gel electrophoresis discrepancy among Pseudomonas aeruginosa isolates from a cystic fibrosis patient.
A multilocus sequence type (MLST) shift (from ST242 to ST996) was detected in Pseudomonas aeruginosa isolates with a uniform pulsed-field gel electrophoresis (PFGE) pattern obtained from a chronically colonized patient. MLST mutational change involved the mutL gene with the consequent emergence of a hypermutable phenotype. This observation challenges the required neutrality of mutL as an appropriate marker in MLST and alerts researchers to the limitations of MLST-only-based population studies in chronic infections under constant antibiotic selective pressure.
Servicio de Microbiología and CIBER en Epidemiología y Salud Pública, Instituto Ramón y Cajal de Investigación Sanitaria, Hospital Universitario Ramón y Cajal, Madrid, Spain.
María
M
García-Castillo
Luis
L
Máiz
María-Isabel
MI
Morosini
Mercedes
M
Rodríguez-Baños
Lucrecia
L
Suarez
Ana
A
Fernández-Olmos
Fernando
F
Baquero
Rafael
R
Cantón
Rosa
R
del Campo
D000251:Adenosine Triphosphatases / Q000235:genetics*; D003550:Cystic Fibrosis / Q000150:complications; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D058885:Multilocus Sequence Typing; D020125:Mutation, Missense; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000145:classification* / Q000201:enzymology* / Q000235:genetics
D000251:Adenosine Triphosphatases