Cloning, expression and evaluation of the efficacy of a recombinant Entamoeba histolytica cysteine proteinase (EhCP4) antigen in minipig.
Cysteine proteinases 4 (EhCP4) of Entamoeba histolytica are considered important for ameba pathogenicity. The recombinant gene was obtained by cloning and expression of the EhCP4 gene in heterologous host Escherichia coli BL-21 (DE3), were used to evaluate their ability to induce immune protective responses in minipig against challenge infection in a minipig-E. histolytica model. There was a 53.16% reduction (P<0.001) in the group of recovery of challenged E. histolytica compared with that in the control group. Specific anti-EhCP4 antibodies from immune protected minipig had significantly higher levels of immunoglobulin G (IgG) (P<0.001). This is a first report demonstrating that a recombinant form of EhCP4 generated in E. coli, to immunize a minipig model of E. histolytica, and there is significant protection. This study may help to understand the EhCP4 for human in the future.
Guiyang College of Traditional Chinese Medicine, Guiyang 550002, Guizhou Province, China. heguangzhi7711@163.com
Guang-Zhi
GZ
He
Yong
Y
Feng
Shu-Xuan
SX
Deng
Chuan-Wei
CW
An
D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D003001:Cloning, Molecular; D057056:Cysteine Proteases / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D004748:Entamoeba histolytica / Q000201:enzymology* / Q000276:immunology* / Q000472:pathogenicity; D004749:Entamoebiasis / Q000517:prevention & control*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D023421:Models, Animal; D016052:Protozoan Vaccines; D011817:Rabbits; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D013552:Swine; D013556:Swine, Miniature
D000953:Antigens, Protozoan; D016052:Protozoan Vaccines; D011994:Recombinant Proteins; D057056:Cysteine Proteases