Impact of yeast-bacteria coinfection on the detection of Candida sp. in an automated blood culture system.
Invasive candidiasis remains a major cause of morbidity and mortality. It is now well known that an early diagnosis contributes to the patients' outcome. Blood cultures, which are the first-line test in case of bloodstream infection suspicion, can be carried out using fungus-selective medium (containing antibiotics) or standard microorganism medium allowing both bacterial and fungal growth. Some patients can suffer from polymicrobial sepsis involving bacteria and yeasts, so we decided to investigate in blood cultures the influence of the presence of bacteria on fungal development. Simulated blood cultures were performed using Candida albicans or C. glabrata coincubated with Escherichia coli or Staphylococcus aureus at different concentrations. The results showed that, in a standard microorganism medium, bacterial growth could hide the fungal development. Thus, in patients at risk of invasive candidiasis, the use of a specific fungal medium could improve the diagnosis and allow an earlier efficient antifungal treatment.
Laboratoire de Parasitologie et Mycologie Médicale, CHU la Miletrie, Poitiers, France. estelle.cateau@chu-poitiers.fr
Estelle
E
Cateau
Anne-Sophie
AS
Cognee
Tri Cong
TC
Tran
Elodie
E
Vallade
Magali
M
Garcia
Sorya
S
Belaz
Catherine
C
Kauffmann-Lacroix
Marie-Helene
MH
Rodier
D001331:Automation; D016470:Bacteremia / Q000382:microbiology*; D001769:Blood / Q000382:microbiology*; D002176:Candida albicans / Q000254:growth & development / Q000302:isolation & purification*; D041221:Candida glabrata / Q000254:growth & development / Q000302:isolation & purification*; D058387:Candidemia / Q000175:diagnosis* / Q000382:microbiology; D060085:Coinfection / Q000382:microbiology*; D003470:Culture Media / Q000737:chemistry*; D004926:Escherichia coli / Q000302:isolation & purification; D006801:Humans; D008828:Microbiological Techniques; D018805:Sepsis / Q000382:microbiology; D013211:Staphylococcus aureus / Q000302:isolation & purification
D003470:Culture Media