The PsbQ protein stabilizes the functional binding of the PsbP protein to photosystem II in higher plants.
PsbP and PsbQ proteins are extrinsic subunits of photosystem II (PSII) and optimize the oxygen evolution reaction by regulating the binding properties of the essential cofactors Ca(2+) and Cl(-). PsbP induces conformational changes around the catalytic Mn cluster required for Ca(2+) and Cl(-) retention, and the N-terminal region of PsbP is essential for this reaction. It was reported that PsbQ partially restores the functional defect of N-terminal truncated PsbP [Ifuku and Sato (2002) Plant Cell Physiol. 43, 1244-1249]; however, the mechanism of this restoration is yet to be clarified. In this study, we demonstrate that PsbQ is able to restore the functional binding of mutated PsbPs. In the presence of PsbQ, ∆15-PsbP, a truncated PsbP lacking 15 N-terminal residues, was able to specifically bind to NaCl-washed spinach PSII membranes and significantly restore the oxygen evolving activity. Furthermore, PsbQ was also able to compensate for the impaired ion-retention of H144A-PsbP, in which a conserved histidine at position 144 in the C-terminal domain was substituted with an alanine. Fourier transform infrared (FTIR) difference spectroscopy showed that PsbQ restored the ability of ∆15- and H144A-PsbP to induce proper conformational changes during S(1) to S(2) transition. These data suggest that the major function of PsbQ is to stabilize PsbP binding, thereby contributing to the maintenance of the catalytic Mn cluster of the water oxidation machinery in higher plant PSII. This article is part of a Special Issue entitled: Photosynthesis Research for Sustainability: from Natural to Artificial.
Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto, Japan.
Shusuke
S
Kakiuchi
Chihiro
C
Uno
Kunio
K
Ido
Taishi
T
Nishimura
Takumi
T
Noguchi
Kentaro
K
Ifuku
Fumihiko
F
Sato
D045332:Photosystem II Protein Complex / Q000737:chemistry* / Q000502:physiology*; D010940:Plant Proteins / Q000737:chemistry* / Q000502:physiology*; D011487:Protein Conformation; D021122:Protein Subunits / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D018724:Spinacia oleracea / Q000378:metabolism*
D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D021122:Protein Subunits