Polymeric optodes based on upconverting nanorods for fluorescent measurements of pH and metal ions in blood samples.
Optical thin films incorporating NaYF(4):Er,Yb upconverting nanorods and chromoionophore ETH 5418 in hydrophobic polymer matrixes have been developed for the first time to measure pH and metal ions based on the ion-exchange mechanism. The absorption spectra of protonated and unprotonated ETH 5418 overlap the two emission peaks of upconverting material, respectively, which makes the inert nanorods ion-sensitive. Optodes for pH and metal ions (Na(+), K(+), Ca(2+), and Cu(2+)) were investigated and exhibited excellent sensitivity, selectivity, and reproducibility. Because of excitation by the 980 nm laser source, detection in the near-infrared region at 656 nm, and high quantum yield of the nanorods in hydrophobic membrane, the proposed sensors have been successfully used in whole blood measurements with minimized background absorption and sample autofluorescence.
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, China.
Liangxia
L
Xie
Yu
Y
Qin
Hong-Yuan
HY
Chen
D015374:Biosensing Techniques; D005453:Fluorescence; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008670:Metals / Q000097:blood*; D053758:Nanoparticles / Q000737:chemistry*; D043942:Nanotubes; D011108:Polymers / Q000737:chemistry*; D014961:X-Ray Diffraction
D008670:Metals; D011108:Polymers