Galvanostatic entrapment of penicillinase into polytyramine films and its utilization for the potentiometric determination of penicillin.
A sensitive and reliable potentiometric biosensor for determination of penicillin has been developed by exploiting the self-limiting growth of the non-conducting polymer, polytyramine. Optimum polytyramine-penicillinase (PTy-PNCnase) films for potentiometric detection of penicillin were accomplished with monomer solutions which contained 0.03 M tyramine, 37 U/mL penicillinase, 0.01 M KNO3, and 3 mM penicillin with an applied current density of 0.8 mA/cm2 and an electropolymerisation time of 40 seconds. The potentiometric biosensor gave a linear concentration range of 3-283 μM for penicillin and achieved a minimum detectable concentration of 0.3 μM. The biosensor was successfully utilized for the detection of Amoxycillin and gave an average percentage recovery of 102±6%. Satisfactory recoveries of penicillin G were also achieved in milk samples with the potentiometric biosensor when concentrations are ≥20 ppm.
NanoScience and Sensor Technology Research Group, School of Applied Sciences and Engineering, Monash University, Churchill, Vic 3842, Australia. fatma.ismail@sci.monash.edu.au
Fatma
F
Ismail
Samuel B
SB
Adeloju
D000658:Amoxicillin / Q000032:analysis; D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D004800:Enzymes, Immobilized / Q000737:chemistry; D008892:Milk / Q000737:chemistry*; D010400:Penicillin G / Q000032:analysis; D010405:Penicillinase / Q000737:chemistry*; D011199:Potentiometry / Q000379:methods*; D014439:Tyramine / Q000737:chemistry*
D004800:Enzymes, Immobilized; D000658:Amoxicillin; D010405:Penicillinase; D010400:Penicillin G; D014439:Tyramine