Immunohistochemical features of 3,3',4,4'-tetrachloroazobenzene-induced rat gingival lesions.
Gingival lesions of squamous hyperplasia, cystic keratinizing hyperplasia (CKH), and squamous cell carcinoma (SCC) can be induced in rats treated by chronic gavage with 10-100 mg/kg 3,3',4,4'-tetrachloroazobenzene. We evaluated gingival squamous hyperplasia (GSH), CKH, and SCC for the immunohistochemical pattern of expression of carcinogenesis-associated markers. The 3 types of lesions and controls were stained with proliferation markers (proliferating cell nuclear antigen [PCNA] and cyclin-D1), tumor-suppressor markers (β-catenin and mammary serine protease inhibitor [maspin]) and stroma-related markers (α-smooth muscle actin [SMA] and osteonectin/SPARC). The lesions had common immunohistochemical characteristics that differed in their expression patterns among the various diagnoses. PCNA and cyclin-D1 expression was higher in GSH, CKH, and SCC than in controls. The normal membranous expression of β-catenin was lower in GSH, and almost absent in CKH and SCC. Maspin expression was similar in GSH and controls, whereas both CKH and SCC showed decreased expression. SMA and/or osteonectin/SPARC were seen in stromal cells in CKH and SCC. Collectively, there appears to be a progression from hyperplastic and cystic lesions toward malignancy based on the morphological changes, supported by the expression of carcinogenesis-associated proteins. The exact sequence of events leading to SCC remains to be defined in a time-dependent manner.
Hadassah-Hebrew University Medical Center, Jerusalem, Israel.
Yuval
Y
Ramot
Marilena
M
Vered
David E
DE
Malarkey
Michelle J
MJ
Hooth
J Todd
JT
Painter
Dan
D
Dayan
Natasha
N
Clayton
Tiwanda
T
Masinde
Abraham
A
Nyska
D000704:Analysis of Variance; D000818:Animals; D001391:Azo Compounds / Q000633:toxicity*; D014408:Biomarkers, Tumor / Q000737:chemistry / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000139:chemically induced* / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D002722:Chlorobenzenes / Q000633:toxicity*; D019938:Cyclin D1 / Q000737:chemistry / Q000378:metabolism; D004848:Epithelium / Q000737:chemistry / Q000378:metabolism; D005260:Female; D005881:Gingiva / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D005887:Gingival Neoplasms / Q000139:chemically induced* / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D006965:Hyperplasia / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D007150:Immunohistochemistry; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D018709:Statistics, Nonparametric
D001391:Azo Compounds; D014408:Biomarkers, Tumor; C530106:Ccnd1 protein, rat; D002722:Chlorobenzenes; D018809:Proliferating Cell Nuclear Antigen; D019938:Cyclin D1; C013279:3,4,3',4'-tetrachloroazobenzene