[Lactobacillus plantarum and phosprenyl competitively distinguish ovalbumin oligomannoside n-glycan].
OBJECTIVE
Demonstration of the ability of native products of lactobacteria to bind mannose containing N-glycans as a display of one of the mechanisms of probiotic behavior of these symbiont microorganism in gastrointestinal tract (GIT).


METHODS
Lactobacillus plantarum 30 grown on selective medium (MRS-agar) and their ultrasound lysates were used in the study. Standard technique of delayed type hypersensitivity (DTH) reaction was used with inactivated Listeria monocytogenes culture as a priming agent.


RESULTS
DTH reaction in vivo has demonstrated that oligomannoside N-glycan of egg albumin is a general acceptor for adhesins that are present in the native preparations of certain L. plantarum strains, as well as for phosprenyl (PHP) immunomodulator--an inhibitor of cell IL-2 reception.


CONCLUSIONS
The data obtained give evidence that mannose specific adhesins that are conserved in lactobacteria preparations have the same binding sites in egg albumin as PHP and, therefore, IL-2.
S M
SM
Sobolev
T N
TN
Nikolaeva
E A
EA
Grigor'eva
A V
AV
Pronin
D000818:Animals; D001667:Binding, Competitive; D041981:Gastrointestinal Tract / Q000382:microbiology*; D006801:Humans; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007155:Immunologic Factors / Q000276:immunology / Q000378:metabolism* / Q000494:pharmacology; D007376:Interleukin-2 / Q000276:immunology / Q000494:pharmacology; D048191:Lactiplantibacillus plantarum / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D008089:Listeria monocytogenes / Q000276:immunology; D051379:Mice; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism*; D010047:Ovalbumin / Q000737:chemistry / Q000276:immunology / Q000378:metabolism* / Q000494:pharmacology; D011106:Polyisoprenyl Phosphates / Q000276:immunology / Q000378:metabolism* / Q000494:pharmacology; D019936:Probiotics / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding
D007155:Immunologic Factors; D007376:Interleukin-2; D009844:Oligosaccharides; D011106:Polyisoprenyl Phosphates; C028569:oligomannoside; C105485:phosprenyl; D010047:Ovalbumin