Manganese oxide nanowire-mediated enzyme-linked immunosorbent assay.
Nanomaterial-based enzyme-linked immunosorbent assay (ELISA) with sufficient sensing specificity is a useful analytical tool for the detection of toxicologically important substances in complicated biological systems. Increasing worldwide demand for nanomaterials and increasing concern on their safe development and use, require a simple, stable, and sensitive detection assay for pathogen evaluation and environmental monitoring. However, this goal is not yet achieved. A design for a hybrid MnO(2) nanowire-ELISA using the sandwich assay format, which provides quantitative binding information for both a specific antibody and the pathogen, sulfate-reducing bacteria, and detects pathogen concentration, is presented. 3,3',5,5'-Tetramethylbenzidine was used as the substrate and was allowed to react with the MnO(2) nanowires without H(2)O(2) in the reaction system. The kinetic parameters were measured with the system acting as a catalytic biosensor. The effectiveness of the MnO(2) nanowire-based biosensor was demonstrated by its sensitive detection of the pathogen.
Key Lab of Corrosion Science, Shandong Province, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China.
Yi
Y
Wan
Peng
P
Qi
Dun
D
Zhang
Jiajia
J
Wu
Yi
Y
Wang
D001419:Bacteria / Q000302:isolation & purification*; D015374:Biosensing Techniques / Q000379:methods*; D002384:Catalysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006735:Horseradish Peroxidase / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D017895:Manganese Compounds / Q000737:chemistry*; D053770:Nanowires; D010087:Oxides / Q000737:chemistry*
D017895:Manganese Compounds; D010087:Oxides; C027424:manganese oxide; D006735:Horseradish Peroxidase