An aptamer based on-plate microarray for high-throughput insulin detection by MALDI-TOF MS.
An aptamer microarray was directly fabricated on a MALDI target plate for high-throughput insulin detection. High sensitivities were observed both in standard solutions (5 ng mL(-1), 0.86 nM) and serum sample (20 ng mL(-1), 3.4 nM). This method shows great promise in the field of biomarker detection.
Department of Chemistry and Institute of Biomedical Science, Fudan University, Shanghai, China.
Xueyang
X
Zhang
Shaochun
S
Zhu
Chunhui
C
Deng
Xiangmin
X
Zhang
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D006046:Gold / Q000737:chemistry; D006801:Humans; D007328:Insulin / Q000032:analysis*; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D040081:Protein Array Analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D052157:Aptamers, Nucleotide; D007328:Insulin; D006046:Gold