Investigation of four porcine candidate genes (H-FABP, MYOD1, UCP3 and MASTR) for meat quality traits in Large White pigs.
Meat quality traits are economically important traits of swine, and are controlled by multiple genes as complex quantitative traits. In the present study four genes, H-FABP (heart fatty acid-binding protein), MASTR (MEF2 activating motif and SAP domain containing transcriptional regulator), UCP3 (uncoupling protein 3) and MYOD1 (myogenic differentiation 1) were researched in Large White pigs. The polymorphisms H-FABP T/C of 5'UTR, MYOD1 g.257 A>C, UCP3 g.1406 G>A in exon 3 and MASTR c.187 C>T have been reported to be associated with meat quality traits in pigs. The aim of this study was to analyze the effect of single and multiple markers for single traits in Large White pigs. The single marker association analysis showed that the H-FABP and MASTR genes were associated with IMF (intramuscular fat content) (P < 0.05), and that the g.257 A>C of MYOD1 gene was most significantly related to muscle pH value (P < 0.01). The multiple markers for IMF were analyzed by combining the markers and quantitative trait modes into the linear regression. The results revealed that H-FABP and MASTR integrate gene networks for IMF. Thus, our study results suggested that H-FABP and MASTR polymorphisms could be used as genetic markers in the marker-assisted selection towards the improvement of IMF in Large White pigs.
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, China.
Xuelei
X
Han
Tengfei
T
Jiang
Huawei
H
Yang
Qingde
Q
Zhang
Weimin
W
Wang
Bin
B
Fan
Bang
B
Liu
D020121:5' Untranslated Regions / Q000235:genetics; D000818:Animals; D050556:Fatty Acid-Binding Proteins / Q000235:genetics*; D005524:Food Technology; D005787:Gene Frequency; D056726:Genetic Association Studies; D005819:Genetic Markers; D005838:Genotype; D007473:Ion Channels / Q000235:genetics*; D016014:Linear Models; D008460:Meat; D024101:Mitochondrial Proteins / Q000235:genetics*; D017570:MyoD Protein / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D034421:Sus scrofa / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics*; D000071247:Uncoupling Protein 3
D020121:5' Untranslated Regions; D050556:Fatty Acid-Binding Proteins; D005819:Genetic Markers; D007473:Ion Channels; D024101:Mitochondrial Proteins; D017570:MyoD Protein; C057363:MyoD1 myogenic differentiation protein; D015534:Trans-Activators; D000071247:Uncoupling Protein 3