Determination of oxytetracycline in milk samples by polymer inclusion membrane separation coupled to high performance liquid chromatography.
The determination of oxytetracycline in milk samples using a polymer inclusion membrane concept with high performance liquid chromatography (HPLC) was studied. The membranes developed are composed by cellulose acetate as polymer base, Cyanex 923 as carrier and o-nitrophenyl octyl ether as plasticizer. In the optimal conditions, the method exhibits good linearity in the range 0.03-0.20 mg L(-1) with a limit of detection and quantification of 8.2 and 27.3 μg L(-1) respectively. The method was successfully applied to the analysis of milk samples with high selectivity.
Universidad Autónoma del Estado de Hidalgo, Área Académica de Química, Hidalgo, Mexico.
Irma
I
Pérez-Silva
José A
JA
Rodríguez
Ma Teresa
MT
Ramírez-Silva
Ma Elena
ME
Páez-Hernández
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000302:isolation & purification*; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D057230:Limit of Detection; D008567:Membranes, Artificial; D008892:Milk / Q000737:chemistry*; D010118:Oxytetracycline / Q000032:analysis / Q000302:isolation & purification*; D011108:Polymers / Q000737:chemistry*
D000900:Anti-Bacterial Agents; D008567:Membranes, Artificial; D011108:Polymers; C005062:acetylcellulose; D002482:Cellulose; D010118:Oxytetracycline