Quality control of modified xiaoyao san through the determination of 22 active components by ultra-performance liquid chromatography.
A simple, sensitive, and reliable ultra-performance liquid chromatography (UPLC) method has been developed for simultaneous determination of 22 major constituents in modified xiaoyao san (MXS), a multiherbal formula. The chromatographic separation was performed on an ACQUITY UPLC BEH C18 column (150 x 2.1 mm, 1.7 microm, particle size), with an aqueous 0.5% acetic acid and acetonitrile mobile phase gradient. The method was validated for linearity (r2 >0.9937), intraday and interday precision (RSD <8.51%), recovery (91.18-107.73%), LOD (0.02-4.17 ng/mL), and LOQ (0.05-12.50 ng/mL). The established method was successfully applied to quantify the 22 marker compounds in MXS, which provided a useful basis of overall evaluation of the quality of MXS.
Sichuan University, Regenerative Medicine Research Center, West China Hospital, Chengdu 610041, People's Republic of China.
Feng
F
Qin
Jun
J
Huang
Xinjian
X
Qiu
Sihang
S
Hu
Xi
X
Huang
D000098:Acetophenones / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000295:instrumentation / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000592:standards; D005960:Glucosides / Q000032:analysis; D006034:Glycyrrhetinic Acid / Q000032:analysis; D019695:Glycyrrhizic Acid / Q000032:analysis; D007529:Isoflavones / Q000032:analysis; D008610:Menthol / Q000032:analysis; D028321:Plant Preparations / Q000737:chemistry / Q000592:standards; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012717:Sesquiterpenes / Q000032:analysis; D052616:Solid Phase Extraction / Q000379:methods
D000098:Acetophenones; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D007529:Isoflavones; D028321:Plant Preparations; D012717:Sesquiterpenes; C516449:xiaoyao; D008610:Menthol; C013638:paeonol; D019695:Glycyrrhizic Acid; C019466:menthone; D006034:Glycyrrhetinic Acid