CNP-1 (ARRD-17), a novel substrate of calcineurin, is critical for modulation of egg-laying and locomotion in response to food and lysine sensation in Caenorhabditis elegans.
Calcineurin is a Ca(2+)/calmodulin-dependent protein phosphatase involved in calcium signaling pathways. In Caenorhabditis elegans, the loss of calcineurin activity causes pleiotropic defects including hyperadaptation of sensory neurons, hypersensation to thermal difference and hyper-egg-laying when worms are refed after starvation. In this study, we report on arrd-17 as calcineurin-interacting protein-1 (cnp-1), which is a novel molecular target of calcineurin. CNP-1 interacts with the catalytic domain of the C. elegans calcineurin A subunit, TAX-6, in a yeast two-hybrid assay and is dephosphorylated by TAX-6 in vitro. cnp-1 is expressed in ASK, ADL, ASH and ASJ sensory neurons as TAX-6. It acts downstream of tax-6 in regulation of locomotion and egg-laying after starvation, ASH sensory neuron adaptation and lysine chemotaxis, that is known to be mediated by ASK neurons. Altogether, our biochemical and genetic evidence indicates that CNP-1 is a direct target of calcineurin and required in stimulated egg-laying and locomotion after starvation, adaptation to hyperosmolarity and attraction to lysine, which is modulated by calcineurin. We suggest that the phosphorylation status of CNP-1 plays an important role in regulation of refed stimulating behaviors after starvation and attraction to amino acid, which provides valuable nutritious information.
Department of Life Science, Gwangju Institute of Science and Technology, Gwangju 500-712, Korea.
Changhoon
C
Jee
Tae-Woo
TW
Choi
Karunambigai
K
Kalichamy
Jong Zin
JZ
Yee
Hyun-Ok
HO
Song
Yon Ju
YJ
Ji
Jungsoo
J
Lee
Jin Il
JI
Lee
Noelle D
ND
L'Etoile
Joohong
J
Ahnn
Sun-Kyung
SK
Lee
D000222:Adaptation, Physiological; D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D019393:Arrestin / Q000737:chemistry; D017173:Caenorhabditis elegans / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D019703:Calcineurin / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005502:Food; D005786:Gene Expression Regulation; D008124:Locomotion / Q000502:physiology*; D008239:Lysine; D008969:Molecular Sequence Data; D009154:Mutation; D009420:Nervous System / Q000254:growth & development; D010058:Oviposition / Q000502:physiology*; D010766:Phosphorylation; D012677:Sensation; D011984:Sensory Receptor Cells; D017386:Sequence Homology, Amino Acid; D020798:Two-Hybrid System Techniques
C572192:ARRD-17 protein, C elegans; D019393:Arrestin; D029742:Caenorhabditis elegans Proteins; D002352:Carrier Proteins; D019703:Calcineurin; D008239:Lysine