Laser capture microdissection of Candida albicans from host tissue.
Laser microdissection is a technique in which specific populations of cells are acquired from sections of complex tissue under direct microscopic visualization. The technique can be used to selectively harvest or ablate host and/or fungal cells from a variety of biological specimens, including human, animal, or plant tissue sections. When coupled with downstream applications such as proteomic and molecular analyses, laser microdissection can address a variety of important biological questions specifically related to the in vivo host-fungus interaction. In this chapter, we describe how laser microdissection enables researchers to selectively isolate Candida albicans cells from host-infected tissue. Detailed protocols are provided for tissue handling and processing, slide preparation, and laser capture microdissection (LCM). Using these methods, we highlight the use of LCM to examine infection-related C. albicans gene expression.
Department of Craniofacial Biology, Medical University of South Carolina, Charleston, SC, USA. westwatc@musc.edu
Caroline
C
Westwater
David A
DA
Schofield
D000818:Animals; D002176:Candida albicans / Q000166:cytology* / Q000235:genetics / Q000302:isolation & purification*; D054884:Host-Pathogen Interactions; D061346:Laser Capture Microdissection / Q000379:methods*; D051379:Mice; D014059:Tongue / Q000166:cytology / Q000382:microbiology*