Hematoporphyrin encapsulated PLGA microbubble for contrast enhanced ultrasound imaging and sonodynamic therapy.
The purpose of this study was to develop a sonosensitizer-loaded multi-functional ultrasound (US) contrast agent for both tumour therapy and imaging. The hematoporphyrin (HP)-encapsulated poly(lactic-co-glycolic acid) microbubbles (HP-PLGA-MBs) were prepared and filled with perfluorocarbon gases. The enhancement of US imaging and its sonodynamically induced anti-tumour effect were evaluated by both in vitro and in vivo experiments. The HP-PLGA-MBs have a narrow size distribution and smooth surface with a mean diameter of 702.6 ± 56.8 nm and HP encapsulation efficiency of 63.50 ± 1.26% and drug-loading efficiency of 2.15 ± 0.13%. The HP-PLGA-MBs could well enhance the ultrasound imaging both in vitro and in vivo. A significant anti-tumour effect was obtained by HP-PLGA-MBs mediated sonodynamic therapy. The tumour growth rate and the tumour proliferation index were the lowest in the HP-PLGA-MBs plus sonication group. And the tumour cell apoptotic index was the biggest in the HP-PLGA-MBs plus sonication group. In conclusion, a sonosensitizer-loaded multi-functional contrast agent was constructed and the feasibility was demonstrated, which might provide a novel strategy for tumour imaging and therapy.
Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China.
Yuanyi
Y
Zheng
Yaping
Y
Zhang
Meng
M
Ao
Ping
P
Zhang
Hui
H
Zhang
Pan
P
Li
Lang
L
Qing
Zhigang
Z
Wang
Haitao
H
Ran
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis / Q000473:pathology / Q000628:therapy*; D045744:Cell Line, Tumor; D003287:Contrast Media / Q000008:administration & dosage / Q000627:therapeutic use*; D004867:Equipment Design; D006415:Hematoporphyrins / Q000008:administration & dosage / Q000627:therapeutic use*; D019344:Lactic Acid / Q000737:chemistry; D008113:Liver Neoplasms / Q000175:diagnosis / Q000473:pathology / Q000628:therapy*; D051379:Mice; D045423:Microbubbles / Q000627:therapeutic use; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D014464:Ultrasonic Therapy / Q000295:instrumentation; D014465:Ultrasonics / Q000295:instrumentation*
D003287:Contrast Media; D006415:Hematoporphyrins; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid