Evaluation of VIDAS Salmonella (SLM) easy Salmonella method for the detection of Salmonella in a variety of foods: collaborative study.
The VIDAS Salmonella (SLM) Easy Salmonella method is a specific enzyme-linked fluorescent immunoassay performed in the automated VIDAS instrument. The VIDAS Easy Salmonella method is a simple 2-step enrichment procedure, using pre-enrichment followed by selective enrichment in a newly formulated broth, SX2 broth. This new method was compared in a multilaboratory collaborative study to the U.S. Food and Drug Administration's Bacteriological Analytical Manual, Chapter 5 method for five food matrixes (liquid egg, vanilla ice cream, spinach, raw shrimp, and peanut butter) and the U.S. Department of Agriculture's Microbiology Laboratory Guidebook 4.04 method for deli turkey. Each food type was artificially contaminated with Salmonella at three inoculation levels. A total of 15 laboratories representing government, academia, and industry, throughout the United States, participated. In this study, 1583 samples were analyzed, of which 792 were paired replicates and 791 were unpaired replicates. Of the 792 paired replicates, 285 were positive by both the VIDAS and reference methods. Of the 791 unpaired replicates, 341 were positive by the VIDAS method and 325 were positive by the cultural reference method. A Chi-square analysis of each of the six food types was performed at the three inoculation levels tested. For all foods evaluated, the VIDAS Easy SLM method demonstrated results comparable to those of the reference methods for the detection of Salmonella.
Q Laboratories, Inc., 1400 Harrison Ave, Cincinnati, OH 45214, USA.
Erin
E
Crowley
Patrick
P
Bird
Kiel
K
Fisher
Katherine
K
Goetz
M Joseph
MJ
Benzinger
James
J
Agin
David
D
Goins
Ronald L
RL
Johnson
D000818:Animals; D010367:Arachis / Q000382:microbiology; D003299:Cooperative Behavior; D003445:Crustacea / Q000382:microbiology; D004531:Eggs / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods*; D005453:Fluorescence; D005516:Food Microbiology / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D007054:Ice Cream / Q000382:microbiology; D007753:Laboratories; D011202:Poultry Products / Q000382:microbiology; D015203:Reproducibility of Results; D012475:Salmonella / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D018724:Spinacia oleracea / Q000382:microbiology; D014481:United States; D014486:United States Food and Drug Administration