Genotypic and phenotypic diversity of PGPR fluorescent pseudomonads isolated from the rhizosphere of sugarcane (Saccharum officinarum L.).
The genetic diversity of plant growth-promoting rhizobacterial (PGPR) fluorescent pseudomonads associated with the sugarcane (Saccharum officinarum L.) rhizosphere was analyzed. Selected isolates were screened for plant growthpromoting properties including production of indole acetic acid, phosphate solubilization, denitrification ability, and production of antifungal metabolites. Furthermore, 16S rDNA sequence analysis was performed to identify and differentiate these isolates. Based on 16S rDNA sequence similarity, the isolates were designated as Pseudomonas plecoglossicida, P. fluorescens, P. libaniensis, and P. aeruginosa. Differentiation of isolates belonging to the same group was achieved through different genomic DNA fingerprinting techniques, including randomly amplified polymorphic DNA (RAPD), amplified ribosomal DNA restriction analysis (ARDRA), repetitive extragenic palindromic (REP), enterobacterial repetitive intergenic consensus (ERIC), and bacterial repetitive BOX elements (BOX) analyses. The genetic diversity observed among the isolates and rep-PCR-generated fingerprinting patterns revealed that PGPR fluorescent pseudomonads are associated with the rhizosphere of sugarcane and that P. plecoglossicida is a dominant species. The knowledge obtained herein regarding the genetic and functional diversity of fluorescent pseudomonads associated with the sugarcane rhizosphere is useful for understanding their ecological role and potential utilization in sustainable agriculture.
Department of Genetics, Center for Excellence in Genomic Sciences, School of Biological Sciences, Madurai Kamaraj University, Madurai 625 021, India.
Neelamegam
N
Rameshkumar
Niraikulam
N
Ayyadurai
Nagarajan
N
Kayalvizhi
Paramasamy
P
Gunasekaran
D000935:Antifungal Agents / Q000378:metabolism; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D058440:Denitrification; D014644:Genetic Variation; D007210:Indoleacetic Acids / Q000378:metabolism; D008969:Molecular Sequence Data; D010710:Phosphates / Q000378:metabolism; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000378:metabolism*; D018517:Plant Roots / Q000382:microbiology*; D010955:Plasmalogens; D011549:Pseudomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D031786:Saccharum / Q000254:growth & development / Q000382:microbiology*; D017422:Sequence Analysis, DNA
D000935:Antifungal Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D007210:Indoleacetic Acids; D010710:Phosphates; D010937:Plant Growth Regulators; D010955:Plasmalogens; D012336:RNA, Ribosomal, 16S; C030737:indoleacetic acid