Performance of two malaria rapid diagnostic tests in febrile adult patients with and without human immunodeficiency virus-1 infection in Blantyre, Malawi.
The performance of two histidine-rich protein type-2-based malaria rapid diagnostic tests (mRDTs) was examined in a rural area with a high prevalence of malaria and human immunodeficiency virus type-1 (HIV-1) infection in 113 and 445 febrile patients ≥ 15 years of age with and without HIV-1 infection, respectively. Patients were tested for HIV-1 infection by using a standard assay and for Plasmodium falciparum by using two mRDTs and microscopy. When microscopy was used as the gold standard, both mRDTs performed similarly in patients with and without HIV-1 infection: Bioline SD Malaria Antigen P.f, sensitivity 94.4% (95% confidence interval [CI]: 81.3-99.3%) versus 97.1% (95% CI:92.8-99.2%) and specificity 50.6% (95% CI: 39.0-62.2%) versus 47.2% (95% CI: 41.4-53.1%); and ICT diagnostics Malaria Pf, sensitivity 94.4% (95% CI: 81.3-99.3%) versus 97.1% (95% CI: 92.8-99.2%) and specificity 50.6% (95% CI:39.0-62.2%) versus 50.3% (95% CI: 44.4-56.1%). Infection with HIV-1 does not appear to affect the performance of these histidine-rich protein type-2 (HRP-2)-based mRDTs.
Jobiba
J
Chinkhumba
Monica
M
Nyanda
Jacek
J
Skarbinski
Don P
DP
Mathanga
D000293:Adolescent; D000328:Adult; D000953:Antigens, Protozoan / Q000032:analysis; D060085:Coinfection / Q000150:complications / Q000453:epidemiology*; D003430:Cross-Sectional Studies; D003955:Diagnostic Tests, Routine / Q000379:methods*; D005260:Female; D005334:Fever / Q000469:parasitology / Q000821:virology; D015658:HIV Infections / Q000150:complications / Q000453:epidemiology*; D015497:HIV-1 / Q000472:pathogenicity; D006801:Humans; D016778:Malaria, Falciparum / Q000150:complications / Q000175:diagnosis* / Q000453:epidemiology*; D008295:Malawi / Q000453:epidemiology; D008297:Male; D008853:Microscopy; D010963:Plasmodium falciparum / Q000472:pathogenicity; D015800:Protozoan Proteins / Q000032:analysis; D012680:Sensitivity and Specificity; D055815:Young Adult
D000953:Antigens, Protozoan; C514457:HRP-2 antigen, Plasmodium falciparum; D015800:Protozoan Proteins