Determination of aflatoxin M1 in milk by high-performance liquid chromatography in Mashhad (north east of Iran).
The aim of this study was to evaluate aflatoxin M1 (AFM1) contamination in milk samples in Mashhad in Iran. A total of 60 milk samples were collected from retail stores of five regions in June 2011. The occurrence and concentration range of AFM1 in the samples were investigated by high-performance liquid chromatography method. AFM1 was found in 100% of the examined milk samples by average concentration of 16.16 ng/L and the contamination level ranging between 2 and 64 ng/L. The concentration of AFM1 in all the samples was lower than the Iranian national standard and Food and Drug Administration limit (500 ng/L), and only in one (1.6%) of the samples, the concentration of AFM1 was greater than the maximum tolerance limit (50 ng/L) accepted by European Union and Codex Alimentarius Commission. Statistical evaluation showed no significant difference between the mean concentrations of AFM1 in milk samples taken from different regions (p > 0.05).
Department of Food Science and Technology, Islamic Azad University, Quchan Branch, Iran. msani@iauq.ac.ir
A Mohamadi
AM
Sani
H
H
Nikpooyan
D016607:Aflatoxin M1 / Q000032:analysis*; D000704:Analysis of Variance; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D059022:Food Safety; D007492:Iran; D016014:Linear Models; D008892:Milk / Q000737:chemistry* / Q000592:standards
D016607:Aflatoxin M1