A resveratrol analog, phoyunbene B, induces G2/M cell cycle arrest and apoptosis in HepG2 liver cancer cells.
Among the seven natural resveratrol analogs separated and identified from Pholidota yunnanensis R(OLFE), we found phoyunbene B (PYB, trans-3,4'-dihydroxy-2',3',5-trimethoxystilbene) was more effective in inhibiting the growth of HepG2 hepatocellular carcinoma cells than resveratrol. The inhibitory effect of PYB in HepG2 cells was due to its induction of G2/M cell cycle arrest and apoptosis. Induction of G2/M phase cell cycle arrest by PYB was associated with its up-regulation of Cyclin B1, while its induction of apoptosis was accompanied with its down-regulation of Bcl-2 and up-regulation of Bax. Our in vitro invasion/migration assays also showed that PYB could inhibit the invasion of hepatocellular carcinoma cells.
School of Pharmaceutical Sciences, Xiamen University, 422 South Siming Road, Xiamen, Fujian 361005, PR China.
Guanghui
G
Wang
Xiaoyu
X
Guo
Haifeng
H
Chen
Ting
T
Lin
Yang
Y
Xu
Quancheng
Q
Chen
Jie
J
Liu
Jinzhang
J
Zeng
Xiao-Kun
XK
Zhang
Xinsheng
X
Yao
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy*; D059565:G2 Phase Cell Cycle Checkpoints / Q000187:drug effects*; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy*; D059566:M Phase Cell Cycle Checkpoints / Q000187:drug effects; D029595:Orchidaceae / Q000737:chemistry; D000077185:Resveratrol; D013267:Stilbenes / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*
D000972:Antineoplastic Agents, Phytogenic; D013267:Stilbenes; C572671:trans-3,4'-dihydroxy-2',3',5-trimethoxystilbene; D000077185:Resveratrol