Fabrication of a microbial biosensor based on QD-MWNT supports by a one-step radiation reaction and detection of phenolic compounds in red wines.
An Acaligense sp.-immobilized biosensor was fabricated based on QD-MWNT composites as an electron transfer mediator and a microbe immobilization support by a one-step radiation reaction and used for sensing phenolic compounds in commercial red wines. First, a quantum dot-modified multi-wall carbon nanotube (QD-MWNT) composite was prepared in the presence of MWNT by a one-step radiation reaction in an aqueous solution at room temperature. The successful preparation of the QD-MWNT composite was confirmed by XPS, TEM, and elemental analysis. Second, the microbial biosensor was fabricated by immobilization of Acaligense sp. on the surface of the composite thin film of a glassy carbon (GC) electrode, which was prepared by a hand casting method with a mixture of the previously obtained composite and Nafion solution. The sensing ranges of the microbial biosensor based on CdS-MWNT and Cu(2)S-MWNT supports were 0.5-5.0 mM and 0.7-10 mM for phenol in a phosphate buffer solution, respectively. Total concentration of phenolic compounds contained in commercial red wines was also determined using the prepared microbial immobilized biosensor.
Department of Chemistry, Hannam University, Daejeon 305-811, Korea. 332816@hanmail.net
Seul-Ki
SK
Kim
Hai-Doo
HD
Kwen
Seong-Ho
SH
Choi
D001419:Bacteria / Q000378:metabolism*; D015374:Biosensing Techniques / Q000295:instrumentation*; D055664:Electrochemical Techniques; D005720:Gamma Rays; D006863:Hydrogen-Ion Concentration / Q000528:radiation effects; D055616:Microtechnology / Q000379:methods*; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D010636:Phenols / Q000032:analysis*; D056951:Photoelectron Spectroscopy; D045663:Quantum Dots; D014920:Wine / Q000032:analysis*
D037742:Nanotubes, Carbon; D010636:Phenols