Streptomyces nodosus host strains optimized for polyene glycosylation engineering.
The AmphDI glycosyltransferase transfers a mycosaminyl sugar residue from GDP onto 8-deoxyamphoteronolide B, the aglycone of the antifungal amphotericin B. In this study the amphDI gene was inactivated in Streptomyces nodosus strains lacking the AmphN cytochrome P450. The new mutants produced 8-deoxy-16-methyl-16-descarboxyl amphoteronolides in high yield. These strains and aglycones should prove valuable for in vivo and in vitro glycosylation engineering.
School of Biomolecular and Biomedical Science, Centre for Synthesis and Chemical Biology, University College Dublin, Belfield, Dublin, Ireland.
Niamh
N
Stephens
Bernard
B
Rawlings
Patrick
P
Caffrey
D000666:Amphotericin B / Q000096:biosynthesis*; D003577:Cytochrome P-450 Enzyme System; D006031:Glycosylation; D018942:Macrolides; D009154:Mutation; D011090:Polyenes / Q000737:chemistry; D015202:Protein Engineering / Q000379:methods*; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism*
D018942:Macrolides; D011090:Polyenes; D000666:Amphotericin B; D003577:Cytochrome P-450 Enzyme System