Metabolism of hyperpolarized [1-¹³C]pyruvate in the isolated perfused rat lung - an ischemia study.
We report studies of the effect of ischemia on the metabolic activity of the intact perfused lung and its restoration after a period of reperfusion. Two groups of rat lungs were studied using hyperpolarized 1-(13) C pyruvate to compare the rate of lactate labeling differing only in the temporal ordering of ischemic and normoxic acquisitions. In both cases, a several-fold increase in lactate labeling was observed immediately after a 25-min ischemia event as was its reversal back to the baseline after 30-40 min of resumed perfusion (n = 5, p < 0.025 for both comparisons). These results were corroborated by (31) P spectroscopy and correspond well to measured changes in lactate pool size determined by (1) H spectroscopy of freeze-clamped specimens.
Department of Radiology, University of Pennsylvania, Philadelphia, PA, United States.
B
B
Pullinger
H
H
Profka
J H
JH
Ardenkjaer-Larsen
N N
NN
Kuzma
S
S
Kadlecek
R R
RR
Rizi
D000818:Animals; D002247:Carbon Isotopes; D066298:In Vitro Techniques; D007511:Ischemia / Q000378:metabolism*; D007553:Isotope Labeling; D019344:Lactic Acid / Q000378:metabolism; D008168:Lung / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D010477:Perfusion / Q000379:methods*; D025341:Principal Component Analysis; D019289:Pyruvic Acid / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015424:Reperfusion
D002247:Carbon Isotopes; D019344:Lactic Acid; D019289:Pyruvic Acid