Identification of enzyme-converted peptide products from single cells using capillary electrophoresis and liquid chromatography-mass spectrometry.
Single-cell analysis using chemical methods, otherwise known as chemical cytometry, promises to provide significant leaps in understanding signaling processes which result in cellular behavior. Sensitive methods for chemical cytometry such as capillary electrophoresis can detect and quantify multiple targets; however, conclusive identification of detected analytes is required for useful data to be obtained. Here, we demonstrate a method for determining the identity of enzyme-converted peptide products from single cells using a combination of capillary electrophoresis and liquid chromatography-mass spectrometry (LC-MS).
Institute of Biomaterials and Biomedical Engineering (IBBME), University of Toronto, Toronto, ON, Canada.
Robert B
RB
Brown
Johannes A
JA
Hewel
Andrew
A
Emili
Julie
J
Audet
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002853:Chromatography, Liquid / Q000379:methods; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D059010:Single-Cell Analysis / Q000295:instrumentation / Q000379:methods*
D005456:Fluorescent Dyes; D010455:Peptides