[Selenium determination in plasma/serum by inductively coupled plasma mass spectrometry (ICP-MS): comparison with graphite furnace atomic absorption spectrometry (GF-AAS)].
BACKGROUND
The present study was aimed at comparing two techniques of selenium (Se) determination in serum/plasma samples: inductively coupled plasma mass spectrometry (ICP-MS) and graphite furnace atomic absorption (GF-AAS).


METHODS
Blood samples were collected by venipuncture, using Venosafe closed blood sampling system. The samples were centrifuged. The measurements were performed by Elan DRC-e mass spectrometry, Perkin Elmer, SCIEX, USA and Unicam Solar 989 QZ atomic absorption spectrometry. Reference material, Clincheck Serum Control Level 1 (Recipe, Germany), was used to verify the determinations. The Laboratory participates in external quality control (G-EQUAS).


RESULTS
Analytical parameters for both techniques are respectively: ICP-MS--precision 5.9%, limit of detection 0.19 microg/l, repeatability 5.5%, trueness 2.4%, bias 97.6%, GF-AAS--precision 8%, limit of detection 3.4 microg/l, repeatability 7.2%, trueness 6.8%, bias 93.2%.


CONCLUSIONS
The benefits of the ICP-MS technique are high accuracy, low detection limits and the possibility of multi-element analysis.
Zakład Bezpieczeństwa Chemicznego, Instytut Medycyny Pracy im. prof. J. Nofera, Łódź. beatajan@imp.lodz.pl
Beata
B
Janasik
Małgorzata
M
Trzcinka-Ochocka
Renata
R
Brodzka
D001774:Blood Chemical Analysis; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D009784:Occupational Diseases / Q000097:blood*; D012643:Selenium / Q000032:analysis* / Q000097:blood; D013054:Spectrophotometry, Atomic / Q000379:methods
D012643:Selenium