Microfluidic nanoplasmonic-enabled device for multiplex DNA detection.
We describe a rapid, quantitative, multiplex, self-labelled, and real-time DNA biosensor employing Ag nanoparticle-bound DNA hairpin probes immobilized in a microfluidic channel. Capture of complementary target DNAs by the microarrayed DNA hairpin probes results in a positive fluorescence signal via a conformational change of the probe molecules, signalling the presence of target DNAs. The device's capability for quantitative analyses was evaluated and a detection time as low as 6 min (with a target flow rate of 0.5 μl min(-1)) was sufficient to generate significant detection signals. This detection time translates to merely 3 μl of target solution consumption. An unoptimized sensitivity of 500 pM was demonstrated for this device.
Department of Biomedical Engineering, University of Rochester, Rochester, NY 14627, USA.
Hsin-I
HI
Peng
Christopher M
CM
Strohsahl
Benjamin L
BL
Miller
D001408:Bacillus anthracis / Q000235:genetics; D015374:Biosensing Techniques / Q000191:economics / Q000295:instrumentation*; D004247:DNA / Q000032:analysis*; D015342:DNA Probes / Q000737:chemistry; D004269:DNA, Bacterial / Q000032:analysis; D004867:Equipment Design; D005453:Fluorescence; D046210:Microfluidic Analytical Techniques / Q000191:economics / Q000295:instrumentation*; D053758:Nanoparticles / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012834:Silver / Q000737:chemistry*; D013997:Time Factors
D015342:DNA Probes; D004269:DNA, Bacterial; D012834:Silver; D004247:DNA