Fresh green tea and gallic acid ameliorate oxidative stress in kainic acid-induced status epilepticus.
Green tea is one of the most-consumed beverages due to its taste and antioxidative polyphenols. However, the protective effects of green tea and its constituent, gallic acid (GA), against kainic acid (KA)-induced seizure have not been studied. We investigated the effect of fresh green tea leaf (GTL) and GA on KA-induced neuronal injury in vivo and in vitro. The results showed that GTL and GA reduced the maximal seizure classes, predominant behavioral seizure patterns, and lipid peroxidation in male FVB mice with status epilepticus (SE). GTL extract and GA provided effective protection against KA-stressed PC12 cells in a dose-dependent manner. In the protective mechanism study, GTL and GA decreased Ca(2+) release, ROS, and lipid peroxidation from KA-stressed PC12 cells. Western blot results revealed that mitogen-activated protein kinases (MAPKs), RhoA, and COX-2 expression were increased in PC12 cells under KA stress, and expression of COX-2 and p38 MAPK, but not RhoA, was significantly reduced by GTL and GA. Furthermore, GTL and GA were able to reduce PGE(2) production from KA-stressed PC12 cells. Taken together, the results showed that GTL and GA provided neuroprotective effects against excitotoxins and may have a clinical application in epilepsy.
Department of Healthcare Management, Yuanpei University, Hsinchu, Taiwan.
Hsiao-Ling
HL
Huang
Chih-Cheng
CC
Lin
Kee-Ching G
KC
Jeng
Pei-Wun
PW
Yao
Lu-Te
LT
Chuang
Su-Ling
SL
Kuo
Chien-Wei
CW
Hou
D000818:Animals; D000975:Antioxidants; D002118:Calcium / Q000378:metabolism; D005707:Gallic Acid / Q000494:pharmacology*; D007608:Kainic Acid / Q000633:toxicity; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D051379:Mice; D018696:Neuroprotective Agents; D018384:Oxidative Stress / Q000187:drug effects*; D016716:PC12 Cells; D010936:Plant Extracts / Q000494:pharmacology; D051381:Rats; D013226:Status Epilepticus / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism*; D013662:Tea / Q000737:chemistry*
D000975:Antioxidants; D018696:Neuroprotective Agents; D010936:Plant Extracts; D013662:Tea; D005707:Gallic Acid; D007608:Kainic Acid; D002118:Calcium