Improvement of the transcriptional strength of baculovirus very late polyhedrin promoter by repeating its untranslated leader sequences and coexpression with the primary transactivator.
Modified polyhedrin promoter (Ppolh) was designed by repeating burst sequences (BSs) and adopted to overexpress rat α2,6-sialyltransferase (ST6Gal I) in silkworm. Modified Ppolh of five BSs with VLF-1 coexpression yielded 2.9 U/ml ST6Gal I activity and 32.5 mU/mg specific activity, which was 1.7- and 2.3-fold higher, respectively compared to Ppolh.
Laboratory of Biotechnology, Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Shizuoka 422-8529, Japan.
Tatsuya
T
Kato
Suganthi Lavender
SL
Manohar
Shin
S
Kanamasa
Makoto
M
Ogata
Enoch Y
EY
Park
D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D057005:Bioengineering; D012831:Bombyx / Q000235:genetics / Q000378:metabolism / Q000821:virology; D004268:DNA-Binding Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D051381:Rats; D012045:Regulatory Sequences, Nucleic Acid; D012799:Sialyltransferases / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase
D004268:DNA-Binding Proteins; D015534:Trans-Activators; D014764:Viral Proteins; D012799:Sialyltransferases; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase