Single-cell electroporation using proton beam fabricated biochips.
We report the design and fabrication of a novel single cell electroporation biochip featuring high aspect ratio nickel micro-electrodes with smooth side walls between which individual cells are attached. The biochip is fabricated using Proton Beam Writing (PBW), a new direct write lithographic technique capable of fabricating high quality high-aspect-ratio nano and microstructures. By applying electrical impulses across the biochip electrodes, SYTOX® Green nucleic acid stain is incorporated into mouse neuroblastoma (N2a) cells and observed via green fluorescence when the stain binds with DNA inside the cell nucleus. Three parameters; electric field strength, pulse duration, and numbers of pulses have been investigated for the single cell electroporation process. The results indicate high transfection rates as well as cell viability of 82.1 and 86.7% respectively. This single cell electroporation system may represent a promising method for the introduction of a wide variety of fluorophores, nanoparticles, quantum dots, DNAs and proteins into cells.
Prince of Songkla University, Department of Physics, Hat Yai, Songkhla 90112, Thailand. hsureerat@hotmail.com
S
S
Homhuan
B
B
Zhang
F-S
FS
Sheu
A A
AA
Bettiol
F
F
Watt
D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D004247:DNA / Q000737:chemistry; D004566:Electrodes; D018274:Electroporation / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D005453:Fluorescence; D051379:Mice; D046228:Microarray Analysis / Q000295:instrumentation* / Q000379:methods; D055616:Microtechnology / Q000295:instrumentation / Q000379:methods; D009447:Neuroblastoma / Q000175:diagnosis; D011522:Protons; D014162:Transfection
D011522:Protons; D004247:DNA