Synergism between microwave irradiation and enzyme catalysis in transesterification of ethyl-3-phenylpropanoate with n-butanol.
Lipase catalyzed transesterification was investigated to study the synergistic effect of microwave irradiation and enzyme catalysis. Transesterification of ethyl-3-phenylpropanoate with n-butanol was chosen as the model reaction using immobilized enzymes such as Novozyme 435, Lipozyme RMIM and Lipozyme TL IM with microwave irradiation. Novozyme 435 was the best catalyst. The effect of various parameters affecting the conversion and initial rates of transesterification were studied to establish kinetics and mechanism. There is synergism between enzyme catalysis and microwave irradiation. The analysis of initial rate data and progress curve data showed that the reaction obeys the Ping-Pong bi-bi mechanism with inhibition by n-butanol. The theoretical predictions and experimental data match very well. These studies were also extended to other alcohols such as 2-phenyl-1-propanol, n-octanol, benzyl alcohol, iso-amyl alcohol, 2-hexanol and 2-pentanol under otherwise similar conditions.
Department of Chemical Engineering, Institute of Chemical Technology, University of Mumbai,(1) Matunga, Mumbai 400 019, India. gdyadav@yahoo.com,
Ganapati D
GD
Yadav
Sandip V
SV
Pawar
D020001:1-Butanol / Q000378:metabolism*; D000438:Alcohols / Q000494:pharmacology; D055162:Biocatalysis / Q000187:drug effects; D004800:Enzymes, Immobilized; D004951:Esterification / Q000187:drug effects; D005656:Fungal Proteins; D006358:Hot Temperature; D007700:Kinetics; D008049:Lipase / Q000378:metabolism*; D008872:Microwaves; D010666:Phenylpropionates / Q000378:metabolism*; D059027:Recycling; D012997:Solvents / Q000494:pharmacology
D000438:Alcohols; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D010666:Phenylpropionates; D012997:Solvents; C469627:ethyl phenylpropanoate; D020001:1-Butanol; C093666:Lipozyme; C093667:Novozyme 435; D008049:Lipase