Evaluation of selective tumor detection by clinical magnetic resonance imaging using antibody-conjugated superparamagnetic iron oxide.
Active targeting by monoclonal antibodies (mAbs) combined with nanosize superparamagnetic iron oxide (SPIO) is a promising technology for magnetic resonance imaging (MRI) diagnosis. However, the clinical applicability of this technology has not been investigated using appropriate controls. It is important to evaluate the targeting technology using widely used clinical 1.5-Tesla MRI in addition to the high-Tesla experimental MRI. In this study, we measured mAb-conjugated dextran-coated SPIO nanoparticles (CMDM) in vivo using clinical 1.5-Tesla MRI. MRI of tumor-bearing mice was performed using a simple comparison between positive and negative tumors derived from the same genetic background in each mouse. The system provided significant tumor-targeting specificity of the target tumor. To the best of our knowledge, this is the first report on the specific detection of target tumors by mAb-conjugated SPIO using clinical 1.5-Tesla MRI. Our observations provide clues for reliable active targeting using mAb-conjugated SPIO in clinical applications.
Department of Intractable Diseases, Research Institute, National Center for Global Health and Medicine, 1-21-1 Toyama, Shinjuku-ku, Tokyo 162-8655, Japan.
Takayoshi
T
Koyama
Mari
M
Shimura
Yuzuru
Y
Minemoto
Satoshi
S
Nohara
Sayaka
S
Shibata
Yutaka
Y
Iida
Shinki
S
Iwashita
Masakatsu
M
Hasegawa
Tohru
T
Kurabayashi
Hirofumi
H
Hamada
Kenji
K
Kono
Eiichi
E
Honda
Ichio
I
Aoki
Yukihito
Y
Ishizaka
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D045744:Cell Line, Tumor; D003287:Contrast Media / Q000737:chemistry*; D003911:Dextrans / Q000737:chemistry*; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry; D007074:Immunoglobulin G / Q000737:chemistry; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D041681:NIH 3T3 Cells; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000175:diagnosis*; D010316:Particle Size
D000911:Antibodies, Monoclonal; D003287:Contrast Media; D003911:Dextrans; D005456:Fluorescent Dyes; D007074:Immunoglobulin G; D058185:Magnetite Nanoparticles; C097921:ferumoxtran-10; C014392:carboxymethyl dextran