Immunophenotypic evaluation of working Labrador Retrievers and German Shepherd dogs living in the same environment.
Multiparametric flow cytometry was used to compare peripheral blood lymphocyte subset distribution between healthy working police Labrador Retrievers (LRs; n=12) and German Shepherd dogs (GS; n=11) living in the same environment. The CD4/CD8 ratio was significantly higher in LR than in GS because of the lower percentage of CD8+ T lymphocytes in LR. GS showed the highest relative percentage of CD3-/CD21- lymphocytes, whereas LR had the highest percentages of MHC II+ lymphocytes. Because age, sex, environmental and housing conditions, dietary patterns, and training or working routines were similar in both breeds in the study, differences in peripheral blood lymphocyte subset distribution could be attributed to the influence of breed on the immune system.
Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Complutense University of Madrid, Avenida Puerta de Hierro s/n, 28040 Madrid, Spain. alejandrav@vet.ucm.es
Alejandra
A
Villaescusa
Mercedes
M
García-Sancho
Alba M
AM
Delgado
Miguel Ángel
MÁ
Tesouro
Fernando
F
Rodríguez-Franco
Angel
A
Sainz
D000818:Animals; D016516:CD4-CD8 Ratio; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology; D004285:Dogs; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D016130:Immunophenotyping / Q000662:veterinary; D016131:Lymphocyte Subsets / Q000166:cytology / Q000276:immunology*; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology*; D008297:Male; D013030:Spain; D013045:Species Specificity