Anti-HBV activities of Streblus asper and constituents of its roots.
The extracts from leaves, heartwood, barks and roots of the Streblus asper were investigated for anti-HBV activities, separately. The results suggested that the MeOH extracts of the heartwood, barks, and roots exhibited good anti-HBV activities. Further investigations displayed that ethyl acetate and n-butanol soluble parts of their MeOH extracts showed more significant anti-HBV activities. Moreover, a new lignan, together with 11 known compounds, was isolated from n-butanol-soluble part of MeOH extract of the roots of S. asper. The structures were elucidated by spectroscopic methods, including 1D NMR ((1)H NMR, (13)C NMR), 2D NMR (HMQC, HMBC) and HR-EI-MS experiments. Compounds 1-3 were evaluated for their anti-HBV activities. Honokiol showed significant anti-HBV activity with IC(50) values of 3.14μM and 4.74μM for HBsAg and HBeAg with no cytotoxicity respectively, while lamivudine (3TC, positive control) exhibited weak anti-HBV activity with IC(50) values of 11.81μM and 25.80μM for HBsAg and HBeAg respectively.
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, School of Environment and Resource of Guangxi Normal University, Guilin 541004, PR China.
Hong
H
Chen
Jun
J
Li
Qiang
Q
Wu
Xiao-Tao
XT
Niu
Mao-Tong
MT
Tang
Xin-Lan
XL
Guan
Jian
J
Li
Rui-Yun
RY
Yang
Sheng-Ping
SP
Deng
Xiao-Jian
XJ
Su
D000998:Antiviral Agents / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D001713:Biphenyl Compounds / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D056945:Hep G2 Cells; D006509:Hepatitis B / Q000188:drug therapy* / Q000821:virology; D006515:Hepatitis B virus / Q000187:drug effects*; D006801:Humans; D020128:Inhibitory Concentration 50; D019259:Lamivudine / Q000302:isolation & purification / Q000494:pharmacology; D017705:Lignans / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D015394:Molecular Structure; D029586:Moraceae / Q000737:chemistry*; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D018517:Plant Roots / Q000737:chemistry; D018514:Plant Structures; D000498:Allyl Compounds; D010636:Phenols
D000998:Antiviral Agents; D001713:Biphenyl Compounds; D019259:Lamivudine; D017705:Lignans; D010936:Plant Extracts; C005499:honokiol; D000498:Allyl Compounds; D010636:Phenols