The population structure of Lutzomyia verrucarum (Diptera: Psycodidae), a Bartonella bacilliformis and Leishmania peruviana vector in Peru.
The population genetic structure of Lutzomyia verrucarum (Townsend), a sand fly disease vector of Carrion's disease and cutaneous leishmaniasis in the Peruvian Andes, was characterized by sequencing 653 bp of cytochrome b and 1,125 bp of the NADH dehydrogenase subunit 4 genes of its mitochondrial genome. DNA sequence variation within and between valleys was compared in a sample of 220 sand flies from three valleys (Purisima, Huaylas, and Conchucos) and five departments (Amazonas, Cajamarca, Piura, Lima, and Huancavelica). Gene network and phylogenetic analyses indicated a high similarity of haplotypes collected within a single valley (0-0.52% nucleotide divergence). Flies from each valley had unique genotypes not shared with specimens from other valleys or from more distant regions (0.8-3.1% nucleotide divergence). Mountain ranges and geographic distance appear to have impeded migration (N(m) = < 0.18) between valleys and separated populations into discrete genetic units.
Yale School of Public Health, 60 College Street, New Haven, CT 06520, USA. lee.cohnstaedt@ars.usda.gov
Lee W
LW
Cohnstaedt
Abraham G
AG
Caceres
Lorenza
L
Beati
Leonard E
LE
Munstermann
D000818:Animals; D045503:Bartonella bacilliformis / Q000502:physiology*; D045303:Cytochromes b / Q000235:genetics; D005786:Gene Expression Regulation / Q000502:physiology; D007303:Insect Vectors / Q000382:microbiology* / Q000502:physiology; D007891:Leishmania / Q000502:physiology*; D008928:Mitochondria / Q000235:genetics; D010568:Peru; D011157:Population Dynamics; D011576:Psychodidae / Q000235:genetics* / Q000382:microbiology* / Q000502:physiology
D045303:Cytochromes b