Stem sugar accumulation in sweet sorghum - activity and expression of sucrose metabolizing enzymes and sucrose transporters.
Sugar metabolism was studied in sweet sorghum (SSV74) that is known to store sugars in the mature internodes and which is reported to give grain yields twice that of a grain sorghum variety (SPV1616). Comparison of sugar accumulation in these two varieties was carried out at three stages of growth and in the upper and lower internodes. In spite of large differences in the level of sugar accumulation, osmolarity of the sap did not vary as significantly in the two varieties. Significant contribution of variety, stage and internode position was seen for the variation observed in sugar content. Though the activities of sugar metabolizing enzymes namely sucrose synthase (in the synthesis and cleavage directions), sucrose phosphate synthase and invertase (cytoplasmic and vacuolar) also varied in a stage- and internode-specific manner in the two varieties, these enzymes did not contribute significantly to the variation observed in sugar content. Transcriptional expression of one sucrose synthase (SUC1), two sucrose phosphate synthase (SPS2 and SPS3) and a vacuolar invertase (INV3) gene were lower in sweet sorghum as compared to grain sorghum. Sweet sorghum also showed lower expression of two sucrose transporters (SUT1 and SUT4), which correlated to higher sugar accumulation in this variety. Differential expression of the sugar metabolizing enzymes and sucrose transporters in sweet and grain sorghum suggest a role for signaling molecules and transcription factors in regulating sugar accumulation observed in the mature internodes of sweet sorghum, which needs to be investigated.
Botany Department, University of Pune, Pune 411 007, India.
Hilal Ahmad
HA
Qazi
Sharayu
S
Paranjpe
Sujata
S
Bhargava
D000704:Analysis of Variance; D050260:Carbohydrate Metabolism / Q000235:genetics; D004553:Electric Conductivity; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000378:metabolism*; D009994:Osmolar Concentration; D010084:Oxidation-Reduction; D053147:Plant Exudates / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D018547:Plant Stems / Q000201:enzymology* / Q000235:genetics; D045868:Sorghum / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism*; D014158:Transcription, Genetic
D026901:Membrane Transport Proteins; D053147:Plant Exudates; D010940:Plant Proteins; C078729:sucrose transport protein, plant; D013395:Sucrose; D005964:Glucosyltransferases; C022699:sucrose synthase