Effect of active immunization against a recombinant mouse granulocyte-macrophage colony-stimulating factor/somatostatin fusion protein on the growth of mice.
Female BALB/c mice were actively immunized subcutaneously with a recombinant protein of granulocyte-macrophage colony-stimulating factor (GM-CSF) fused with somatostatin (SS) (GM-CSF/SS). Fifty-four days after the primary immunization, the body weight of the immunized mice increased by 4.62% compared with the control (P < 0.05), together with the induction of detectable serum antibodies against SS. The level of serum growth hormone (GH) elevated by 44.54% (P < 0.05) and the mRNA expression of muscular IGF-1 increased by 94% for the GM-CSF/SS-treated mice. The results indicated that the recombinant protein GM-CSF/SS was efficient in inducing specific immunity against SS, subsequently leading to the increase of the GH level by SS neutralization, and ultimately improving the growth of mice.
College of Animal Sciences, Zhejiang University, Hangzhou, People's Republic of China.
Bingbing
B
Wu
Ruili
R
Qi
Bin
B
Li
Taoyan
T
Yuan
Heshan
H
Liu
Jun
J
He
Zhiwei
Z
Lin
Weifen
W
Li
Yan
Y
Fu
Dong
D
Niu
D000822:Animal Husbandry; D000818:Animals; D001323:Autoantibodies / Q000097:blood; D003001:Cloning, Molecular; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000096:biosynthesis / Q000276:immunology*; D013006:Growth Hormone / Q000097:blood; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D058751:Livestock / Q000254:growth & development; D051379:Mice; D008807:Mice, Inbred BALB C; D018482:Muscle, Skeletal / Q000378:metabolism; D055435:Myostatin / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000276:immunology*; D013004:Somatostatin / Q000096:biosynthesis / Q000276:immunology*; D014158:Transcription, Genetic / Q000276:immunology; D014611:Vaccination; D015430:Weight Gain / Q000276:immunology
D001323:Autoantibodies; C525912:Mstn protein, mouse; D055435:Myostatin; D011993:Recombinant Fusion Proteins; D016179:Granulocyte Colony-Stimulating Factor; D013004:Somatostatin; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone