Sodium hypochlorite, chlorhexidine gluconate, and commercial denture cleansers as disinfecting agents against Candida albicans: An in vitro comparison study.
When treating patients who have candidiasis, removable dental appliances in active use should be treated as well. The authors aimed to determine, in vitro, the lowest concentration of sodium hypochlorite that would eliminate Candida albicans biofilm, as well as the effectiveness of additional products against C. albicans. Strains of C. albicans formed biofilms on microtiter plates. Sodium hypochlorite was added in dilutions (1:1 to 1:512) and Peridex was added in concentrations of 25%, 50%, and 100%. The plates were incubated for 30 minutes. One tablet each of Efferdent, Polident for Partials, and Polident for Dentures was dissolved in 200 mL of sterile water and added to additional groups of plates. One group was incubated for 30 minutes; the other was incubated for 18 hours. An XTT spectrophotometric reduction assay measured biofilm metabolic activity. Biofilm activity decreased 100% for all strains exposed to sodium hypochlorite for 30 minutes in concentrations of 1:32 or stronger. Biofilm activity decreased 100% for most strains when treated with 50% or 100% Peridex for 30 minutes and Polident for Dentures for 18 hours. From these results, it appears appropriate for providers to recommend a solution of two teaspoons of sodium hypochlorite in one cup of water (1:25) for 30 minutes to treat dentures contaminated with C. albicans.
Department of Orthodontics, Saudi Aramco Medical Service Organization, Dhahran, Saudi Arabia.
Afaf A
AA
Dahlan
Carl W
CW
Haveman
Gordon
G
Ramage
Jose L
JL
Lopez-Ribot
Spencer W
SW
Redding
D000891:Anti-Infective Agents, Local / Q000008:administration & dosage / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D001881:Borates / Q000008:administration & dosage / Q000494:pharmacology; D002176:Candida albicans / Q000187:drug effects* / Q000254:growth & development; D002710:Chlorhexidine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D003124:Colorimetry; D019551:Dental Disinfectants / Q000008:administration & dosage / Q000494:pharmacology*; D003820:Denture Cleansers / Q000494:pharmacology*; D006801:Humans; D007202:Indicators and Reagents; D008422:Materials Testing; D050296:Microbial Viability / Q000187:drug effects; D009172:Mycology / Q000379:methods; D012973:Sodium Hypochlorite / Q000008:administration & dosage / Q000494:pharmacology*; D013053:Spectrophotometry; D013431:Sulfates / Q000008:administration & dosage / Q000494:pharmacology; D013778:Tetrazolium Salts; D013997:Time Factors
D000891:Anti-Infective Agents, Local; D001881:Borates; D019551:Dental Disinfectants; D003820:Denture Cleansers; D007202:Indicators and Reagents; D013431:Sulfates; D013778:Tetrazolium Salts; C059087:2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide; C102654:Polident; D012973:Sodium Hypochlorite; C010882:chlorhexidine gluconate; D002710:Chlorhexidine