A split ligand for lanthanide binding: facile evaluation of dimerizing proteins.
Luminescence of lanthanides is attractive for biological applications due to its long lifetime and sharp emission profiles. We describe the split display of a lanthanide binding ligand that allows facile evaluation of dimerizing proteins. The split lanthanide ligand is cysteine reactive, and therefore should be readily applicable to a variety of protein systems.
Department of Chemistry and Merkert Center of Chemistry, Boston College, Chestnut Hill, MA 02467, USA.
Yue
Y
Zhao
Jianmin
J
Gao
D050976:Basic-Leucine Zipper Transcription Factors / Q000737:chemistry* / Q000235:genetics; D008024:Ligands; D049449:Luminescence; D009154:Mutation; D010795:Phthalic Acids / Q000737:chemistry*; D055503:Protein Multimerization; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000235:genetics; D013725:Terbium / Q000737:chemistry*
D050976:Basic-Leucine Zipper Transcription Factors; C501391:GCN4 protein, S cerevisiae; D008024:Ligands; D010795:Phthalic Acids; D029701:Saccharomyces cerevisiae Proteins; D013725:Terbium