A simple isocratic HPLC method for the simultaneous determination of bioactive components of Scutellariae radix extract.
Scutellariae Radix, the dried root of Scutellaria baicalensis Georgi, has been widely used in Asian countries for the treatment of dermatitis, diarrhoea, inflammatory disease and hepatic disease. A simple, sensitive and precise reversed-phase liquid chromatographic method with isocratic elution was developed to simultaneously determine four bioactive compounds in Scutellariae Radix: baicalein, baicalin, wogonin and wogonoside. Chromatographic analysis was performed on a YMC Pack Pro C(8) column (150 × 4.6 mm(2), 3 µm), with a mobile phase of 0.1% formic acid : acetonitrile (70 : 30, v/v) at a flow rate of 1.0 mL min(-1), and UV detection at 280 nm. Linear behaviour was observed over the investigated concentration range (0.25-10 µg mL(-1)) for all analytes, with a correlation coefficient of >0.997. The intra- and inter-day precisions were <8.07%, and accuracies were 92.3-102.9%. This method was successfully applied for the analysis of marker compounds for the quality control of Scutellariae Radix extract.
Doping Control Center, Korea Institute of Science and Technology, Chungryang, Seoul, Korea.
M Nurul
MN
Islam
Hye Jin
HJ
Chung
Dong-Hyun
DH
Kim
Hye Hyun
HH
Yoo
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry; D044950:Flavanones / Q000032:analysis; D005419:Flavonoids / Q000032:analysis; D005960:Glucosides / Q000032:analysis; D057230:Limit of Detection; D011786:Quality Control; D015203:Reproducibility of Results; D031364:Scutellaria baicalensis / Q000737:chemistry*
D004365:Drugs, Chinese Herbal; D044950:Flavanones; D005419:Flavonoids; D005960:Glucosides; C038044:baicalin; C006680:baicalein; C473995:wogonoside; C085514:wogonin