Increased stability of glycol-terminated self-assembled monolayers for long-term patterned cell culture.
Self-assembled monolayers (SAMs) are widely used to confine proteins and cells to a pattern to study cellular processes and behavior. To fully explore some of these phenomena, it is necessary to control cell growth and confinement for several weeks. Here, we present a simple method by which protein and cellular confinement to a pattern can be maintained for more than 35 days. This represents a significant increase in pattern stability compared to previous monolayer systems and is achieved using an amide-linked glycol monomer on 50 Å titanium/100 Å gold-coated glass coverslips. In addition, this study provides insight into the method of SAM degradation and excludes interfacial mixing of the monomers and blooming of the adlayer as major mechanisms for SAM degradation.
Department of Chemistry, Washington University in St. Louis, St. Louis, Missouri 63130, United States.
Matthew K
MK
Strulson
Dawn M
DM
Johnson
Joshua A
JA
Maurer
D000577:Amides; D000818:Animals; D016466:CHO Cells; D018929:Cell Culture Techniques / Q000379:methods*; D006224:Cricetinae; D006018:Glycols / Q000737:chemistry*; D008567:Membranes, Artificial; D051379:Mice; D041681:NIH 3T3 Cells; D013499:Surface Properties
D000577:Amides; D006018:Glycols; D008567:Membranes, Artificial