A fluorometric method for evaluation of pharmacological activity against intracellular Leishmania amastigotes.
In this work a simple and novel method to evaluate the efficacy of compounds on intracellular Leishmania amastigotes by using a fluorometric assay has been developed. The new method is sensitive, easy to perform and scalable for high throughput and therefore it could be validated for screening of new anti-leishmanial agents.
Departamento de Parasitología. Facultad de Farmacia, Universidad Complutense de Madrid, Plaza Ramón y Cajal s/n, 28040-Madrid, Spain.
Pablo
P
Bilbao-Ramos
Sergio
S
Sifontes-Rodríguez
María Auxiliadora
MA
Dea-Ayuela
Francisco
F
Bolás-Fernández
D000818:Animals; D000981:Antiprotozoal Agents / Q000494:pharmacology*; D002460:Cell Line; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005470:Fluorometry / Q000379:methods*; D057166:High-Throughput Screening Assays / Q000379:methods; D007891:Leishmania / Q000187:drug effects*; D008264:Macrophages / Q000469:parasitology; D051379:Mice; D021261:Parasitic Sensitivity Tests / Q000379:methods; D012680:Sensitivity and Specificity
D000981:Antiprotozoal Agents