The Drosophila melanogaster Genetic Reference Panel.
A major challenge of biology is understanding the relationship between molecular genetic variation and variation in quantitative traits, including fitness. This relationship determines our ability to predict phenotypes from genotypes and to understand how evolutionary forces shape variation within and between species. Previous efforts to dissect the genotype-phenotype map were based on incomplete genotypic information. Here, we describe the Drosophila melanogaster Genetic Reference Panel (DGRP), a community resource for analysis of population genomics and quantitative traits. The DGRP consists of fully sequenced inbred lines derived from a natural population. Population genomic analyses reveal reduced polymorphism in centromeric autosomal regions and the X chromosome, evidence for positive and negative selection, and rapid evolution of the X chromosome. Many variants in novel genes, most at low frequency, are associated with quantitative traits and explain a large fraction of the phenotypic variance. The DGRP facilitates genotype-phenotype mapping using the power of Drosophila genetics.
Department of Genetics, North Carolina State University, Raleigh, North Carolina 27695, USA. trudy_mackay@ncsu.edu
Trudy F C
TF
Mackay
Stephen
S
Richards
Eric A
EA
Stone
Antonio
A
Barbadilla
Julien F
JF
Ayroles
Dianhui
D
Zhu
Sònia
S
Casillas
Yi
Y
Han
Michael M
MM
Magwire
Julie M
JM
Cridland
Mark F
MF
Richardson
Robert R H
RR
Anholt
Maite
M
Barrón
Crystal
C
Bess
Kerstin Petra
KP
Blankenburg
Mary Anna
MA
Carbone
David
D
Castellano
Lesley
L
Chaboub
Laura
L
Duncan
Zeke
Z
Harris
Mehwish
M
Javaid
Joy Christina
JC
Jayaseelan
Shalini N
SN
Jhangiani
Katherine W
KW
Jordan
Fremiet
F
Lara
Faye
F
Lawrence
Sandra L
SL
Lee
Pablo
P
Librado
Raquel S
RS
Linheiro
Richard F
RF
Lyman
Aaron J
AJ
Mackey
Mala
M
Munidasa
Donna Marie
DM
Muzny
Lynne
L
Nazareth
Irene
I
Newsham
Lora
L
Perales
Ling-Ling
LL
Pu
Carson
C
Qu
Miquel
M
Ràmia
Jeffrey G
JG
Reid
Stephanie M
SM
Rollmann
Julio
J
Rozas
Nehad
N
Saada
Lavanya
L
Turlapati
Kim C
KC
Worley
Yuan-Qing
YQ
Wu
Akihiko
A
Yamamoto
Yiming
Y
Zhu
Casey M
CM
Bergman
Kevin R
KR
Thornton
David
D
Mittelman
Richard A
RA
Gibbs
D000483:Alleles; D000818:Animals; D002503:Centromere / Q000235:genetics; D059006:Chromosomes, Insect / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics*; D055106:Genome-Wide Association Study; D023281:Genomics; D005838:Genotype; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D040641:Quantitative Trait Loci / Q000235:genetics*; D012641:Selection, Genetic / Q000235:genetics; D013217:Starvation / Q000235:genetics; D016615:Telomere / Q000235:genetics; D014960:X Chromosome / Q000235:genetics