Development of an enzyme-linked immunosorbent assay system for the determination of asymmetric dimethylarginine using a specific monoclonal antibody.
We produced a monoclonal antibody (mAb) against N(G),N(G)-dimethyl-L-arginine (asymmetric dimethylarginine: ADMA), an endogenous competitive inhibitor of nitric oxide synthase (NOS), and developed an enzyme-linked immunosorbent assay (ELISA). The competitive ELISA method using the mAb determined 5 nM-100 nM ADMA, and ADMA levels in human plasma and urine were found to be 0.78 µM and 51.3 µmol/g of creatinine respectively.
Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University, Soja, Okayama, Japan.
Miyuki
M
Yokoro
Makiko
M
Suzuki
Machiko
M
Yatani
Hiromi
H
Yamashita
Yoshitaka
Y
Takahashi
Hideaki
H
Tsuji
Masumi
M
Kimoto
D000911:Antibodies, Monoclonal; D001120:Arginine / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood / Q000652:urine; D001667:Binding, Competitive; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards; D006801:Humans; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors
D000911:Antibodies, Monoclonal; C018524:N,N-dimethylarginine; D001120:Arginine; D019001:Nitric Oxide Synthase