Halomonas smyrnensis sp. nov., a moderately halophilic, exopolysaccharide-producing bacterium.
Four Gram-negative, moderately halophilic, exopolysaccharide-producing strains, designated AAD6(T), AAD4, AAD17 and AAD21, were isolated from Çamaltı Saltern Area, a wildlife reserve in Sasalı, İzmir province located in the Aegean Region of Turkey. The isolates grew at an optimum NaCl concentration of 10% (w/v). The major cellular fatty acids were C(16:0), C(18:1)ω7c, C(16:1)ω7c and C(12:0) 3OH, respectively and the predominant lipoquinone was ubiquinone Q-9. The G+C content of the genomic DNA of strains AAD6(T), AAD4, AAD17 and AAD21 was 63.0, 63.3, 62.8 and 62.6 mol %, respectively. Comparative 16S rRNA gene sequence studies showed that the isolates belonged to the genus Halomonas. The DNA-DNA hybridization mean values between the representative strain AAD6(T) and the closely related species Halomonas salina DSM 5928(T), Halomonas halophila DSM 4770(T), Halomonas maura DSM 13445(T), Halomonas organivorans DSM 16226(T), Halomonas elongata DSM 2581(T), Halomonas koreensis JCM 12237(T) and Halomonas nitroreducens LMG 24185, were 40.8, 39.6, 24.2, 23.3, 12.6, 14.5 and 12.2%, respectively. Based on these data the strains represent a novel species of the genus Halomonas for which the name Halomonas smyrnensis sp. nov. is proposed. The type strain is AAD6(T) (= DSM 21644(T) = JCM 15723(T)).
Consiglio Nazionale delle Ricerche (C.N.R.), Istituto di Chimica Biomolecolare, via Campi Flegrei 34, 80078, Pozzuoli (Na), Italy.
Annarita
A
Poli
Consiglio Nazionale delle Ricerche (C.N.R.), Istituto di Chimica Biomolecolare, via Campi Flegrei 34, 80078, Pozzuoli (Na), Italy.
Barbara
B
Nicolaus
The Scientific and Technological Research Council of Turkey, Marmara Research Center, Genetic Engineering and Biotechnology Institute, P.O. Box 21, 41470 Gebze, Kocaeli, Turkey.
Aziz Akın
AA
Denizci
Marmara University, Institute for Graduate Studies in Pure and Applied Sciences, Göztepe Campus, 34722 Kadıköy, İstanbul, Turkey.
Bahar
B
Yavuzturk
Marmara University, Faculty of Engineering, Bioengineering Department, Göztepe Campus, 34722 Kadıköy, İstanbul, Turkey.
Dilek
D
Kazan
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D020632:Halomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D061005:Ponds; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014421:Turkey; D014451:Ubiquinone / Q000032:analysis
D004269:DNA, Bacterial; D005227:Fatty Acids; D011135:Polysaccharides, Bacterial; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C030571:ubiquinone 9