Steroidal glycosides from the rhizomes of Anemarrhena asphodeloides and their antiplatelet aggregation activity.
Five new steroidal glycosides, timosaponin J ( 1), timosaponin K ( 2), (25 S)-karatavioside C ( 5), timosaponin L ( 6), and (25 S)-officinalisnin-I ( 8), together with eight known steroidal saponins, timosaponin E (1) ( 3), purpureagitosid ( 4), timosaponin BII ( 7), timosaponin B III ( 9), anemarrhenasaponin I ( 10), anemarrhenasaponin III ( 11), anemarrhenasaponin A (2) ( 12), and timosaponin A III ( 13), were isolated from the rhizomes of Anemarrhena asphodeloides. Their structures were elucidated on the basis of spectroscopic and chemical evidence. The aglycones of compounds 1 and 2 are new aglycones. Compounds 1- 13 were evaluated for their platelet aggregation activities, and compound 13 exhibited the strongest inhibitory effect on adenosine diphosphate (ADP)-induced platelet aggregation.
Department of Biotechnology, Beijing Institute of Radiation Medicine, No. 27 Taiping Road, Beijing, P. R. China.
Li-ping
LP
Kang
Jie
J
Zhang
Yue
Y
Cong
Bin
B
Li
Cheng-qi
CQ
Xiong
Yang
Y
Zhao
Da-wei
DW
Tan
He-shui
HS
Yu
Zu-yin
ZY
Yu
Yu-wen
YW
Cong
Chao
C
Liu
Bai-ping
BP
Ma
D000244:Adenosine Diphosphate / Q000494:pharmacology; D031403:Anemarrhena / Q000737:chemistry*; D000818:Animals; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D015394:Molecular Structure; D010946:Plants, Medicinal / Q000737:chemistry; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D027343:Rhizome / Q000737:chemistry; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D013256:Steroids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
D004365:Drugs, Chinese Herbal; D006027:Glycosides; D010975:Platelet Aggregation Inhibitors; D012503:Saponins; D013256:Steroids; D000244:Adenosine Diphosphate