Molecular cloning and expression analysis of glutathione reductase gene in Chlamydomonas sp. ICE-L from Antarctica.
A cDNA (GenBank ID: GU395492) encoding cytosolic glutathione reductase (named ICE-LGR) in Antarctic microalgae Chlamydomonas sp. ICE-L was successfully cloned by RT-PCR and rapid amplification of cDNA ends technique (RACE). The expression patterns of ICE-LGR under different salinity stresses were determined by real-time PCR. ICE-LGR cDNA has 1913 bp nucleotides with an open reading frame (ORF) of 1458 bp, encoding 485 amino acid residues. The deduced amino acid sequence shows 79% homology with glutathione reductase (GR) of Chlamydomonas reinhardtii. Activity assessment and mRNA expression analysis results showed that activity and expression level of GR in ICE-L cells were up-regulated under either high or low salinity. Together, our results revealed that ICE-LGR might play an important role in Antarctic ice algae Chlamydomonas sp. ICE-L acclimatizing to polar high salinity environment as well as low salinity. These results provide us valuable information on further investigating the molecular mechanism of ICE-LGR.
Guangdong Provincial Key Lab of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Fisheries College, Guangdong Ocean University, Zhanjiang, Guangdong 524025, PR China. dingyuddd@163.com
Yu
Y
Ding
Ying
Y
Liu
Ji-Chang
JC
Jian
Zao-He
ZH
Wu
Jin-Lai
JL
Miao
D000595:Amino Acid Sequence; D000864:Antarctic Regions; D002696:Chlamydomonas / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant; D005980:Glutathione Reductase / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018749:RNA, Plant / Q000235:genetics; D054712:Salinity; D016415:Sequence Alignment; D013312:Stress, Physiological
D010940:Plant Proteins; D018749:RNA, Plant; D005980:Glutathione Reductase