Stimulated Raman hyperspectral imaging based on spectral filtering of broadband fiber laser pulses.
We demonstrate a technique of hyperspectral imaging in stimulated Raman scattering (SRS) microscopy using a tunable optical filter, whose transmission wavelength can be varied quickly by a galvanometer mirror. Experimentally, broadband Yb fiber laser pulses are synchronized with picosecond Ti:sapphire pulses, and then spectrally filtered out by the filter. After amplification by fiber amplifiers, we obtain narrowband pulses with a spectral width of <3.3 cm(-1) and a wavelength tunability of >225 cm(-1). By using these pulses, we accomplish SRS imaging of polymer beads with spectral information.
Department of Material and Life Science, Graduate School of Engineering, Osaka University, Suita, Osaka, Japan. ozeki@mls.eng.osaka‑u.ac.jp
Yasuyuki
Y
Ozeki
Wataru
W
Umemura
Kazuhiko
K
Sumimura
Norihiko
N
Nishizawa
Kiichi
K
Fukui
Kazuyoshi
K
Itoh
D007834:Lasers; D008853:Microscopy / Q000295:instrumentation*; D055100:Optical Fibers; D013059:Spectrum Analysis, Raman / Q000295:instrumentation*