S100A9 a new marker for monocytic human myeloid-derived suppressor cells.
Myeloid-derived suppressor cells (MDSC) are a heterogeneous population of cells that negatively regulate the immune response during tumour progression, inflammation and infection. Only limited data are available on human MDSC because of the lack of specific markers. We have identified members of the S100 protein family-S100A8, S100A9 and S100A12 - specifically expressed in CD14(+) HLA-DR(-/low) MDSC. S100A9 staining in combination with anti-CD14 could be used to identify MDSC in whole blood from patients with colon cancer. An increase in the population of CD14(+) S100A9(high) MDSC was observed in the peripheral blood from colon cancer patients in comparison with healthy controls. Finally, nitric oxide synthase expression, a hallmark of MDSC, was induced in CD14(+) S100A9(high) upon lipopolysaccharide/interferon-γ stimulation. We propose S100 proteins as useful markers for the analysis and further characterization of human MDSC.
National Institutes of Health, National Cancer Institute, Medical Oncology Branch, Bethesda, MD 20892, USA.
Fei
F
Zhao
Bastian
B
Hoechst
Austin
A
Duffy
Jaba
J
Gamrekelashvili
Suzanne
S
Fioravanti
Michael P
MP
Manns
Tim F
TF
Greten
Firouzeh
F
Korangy
D015415:Biomarkers / Q000097:blood; D040501:Calgranulin A / Q000096:biosynthesis / Q000276:immunology; D040502:Calgranulin B / Q000096:biosynthesis / Q000276:immunology*; D003110:Colonic Neoplasms / Q000097:blood / Q000276:immunology; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D018950:Lipopolysaccharide Receptors / Q000032:analysis / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D022423:Myeloid Cells / Q000276:immunology* / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000096:biosynthesis; D009418:S100 Proteins / Q000096:biosynthesis / Q000276:immunology; D000067616:S100A12 Protein
D015415:Biomarkers; D040501:Calgranulin A; D040502:Calgranulin B; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; D009418:S100 Proteins; D000067616:S100A12 Protein; C090441:S100A12 protein, human; D007371:Interferon-gamma; D019001:Nitric Oxide Synthase