Photoprotective potential of strawberry (Fragaria × ananassa) extract against UV-A irradiation damage on human fibroblasts.
Exposure to UV-A radiation is known to induce discrete lesions in DNA and the generation of free radicals that lead to a wide array of skin diseases. Strawberry (Fragaria × ananassa) contains several polyphenols with strong antioxidant and anti-inflammatory activities. Because the major representative components of strawberry are anthocyanins, these may significantly contribute to its properties. To test this hypothesis, methanolic extracts from the Sveva cultivar were analyzed for anthocyanin content and for their ability to protect human dermal fibroblasts against UV-A radiation, as assayed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenytetrazolium bromide and Comet assays. Five anthocyanin pigments were identified using high-performance liquid chromatography-diode array detection-electrospray ionization/mass spectrometry. Moreover, the strawberry extract showed a photoprotective activity in fibroblasts exposed to UV-A radiation, increasing cellular viability, and diminishing DNA damage, as compared to control cells. Overall, our data show that strawberry contains compounds that confer photoprotective activity in human cell lines and may protect skin against the adverse effects of UV-A radiation.
Dipartimento di Scienze Cliniche Specialistiche ed Odontostomatologiche, Università Politecnica delle Marche, Ancona, Italy.
Francesca
F
Giampieri
Josè M
JM
Alvarez-Suarez
Sara
S
Tulipani
Ana M
AM
Gonzàles-Paramàs
Celestino
C
Santos-Buelga
Stefano
S
Bompadre
José L
JL
Quiles
Bruno
B
Mezzetti
Maurizio
M
Battino
D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects / Q000528:radiation effects*; D031985:Fragaria / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D006801:Humans; D010936:Plant Extracts / Q000494:pharmacology*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D012871:Skin Diseases / Q000209:etiology / Q000517:prevention & control; D014466:Ultraviolet Rays / Q000009:adverse effects*
D010936:Plant Extracts; D011837:Radiation-Protective Agents