Increased miR-222 in H. pylori-associated gastric cancer correlated with tumor progression by promoting cancer cell proliferation and targeting RECK.
Little is known about the potential role of microRNAs (miRNAs) in the carcinogenesis of gastric cancer induced by Helicobacter pylori (H. pylori). Here, we showed that microRNA-222 (miR-222) was up-regulated in H. pylori-infected gastric mucosa and gastric cancer. Ectopic expression of miR-222 promoted cell proliferation and colony formation in vitro. Mechanistically, we identified RECK as a novel target of miR-222, and also confirmed their relationship by the inverse correlation of mRNA expression ex vivo. Furthermore, we found that RNA interference silencing of RECK can mimic the oncogenic effects of miR-222. Collectively, H. pylori may function as an initiator in the process of carcinogenesis by up-regulating miR-222, which further participates in the progression of cancer by promoting proliferation and inhibiting RECK.
Department of Clinical Microbiology and Immunology, College of Medical Laboratory Science, Third Military Medical University, Chongqing 400038, China.
Na
N
Li
Bin
B
Tang
En-Dong
ED
Zhu
Bo-sheng
BS
Li
Yuan
Y
Zhuang
Shu
S
Yu
Dong-shui
DS
Lu
Quan-Ming
QM
Zou
Bin
B
Xiao
Xu-Hu
XH
Mao
D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic; D018450:Disease Progression; D058851:GPI-Linked Proteins / Q000235:genetics / Q000378:metabolism*; D005753:Gastric Mucosa / Q000382:microbiology / Q000473:pathology; D016481:Helicobacter Infections / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D016480:Helicobacter pylori / Q000235:genetics* / Q000472:pathogenicity; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D013274:Stomach Neoplasms / Q000382:microbiology* / Q000473:pathology / Q000503:physiopathology*
D058851:GPI-Linked Proteins; D035683:MicroRNAs; C525710:MIR222, human; C115460:RECK protein, human