In vitro and in vivo efficacy of aqueous extract of Caryocar brasiliense Camb. to control gastrointestinal nematodes in sheep.
A major problem faced in sheep rearing has been the rapid acquisition of anthelminthic-resistant populations of gastrointestinal nematodes. In the search for alternatives, aqueous extract of the peel of Caryocar brasiliense was evaluated for larval development inhibition, egg-hatching inhibition, and fecal nematode egg count reduction in sheep. For in vivo analysis, the doses were calculated according to a 10% lethal dose derived from acute toxicity tests in mice, and the efficacy was evaluated for two periods following oral administration of the extract. Egg-hatching inhibition at concentrations of 15 and 7.5 mg/ml was significantly higher than observed in negative controls with distilled water. For larval development inhibition, all concentrations showed anthelminthic activity significantly higher than controls and were not significantly different from ivermectin treatment. The LC(90) of larval development inhibition was 53.19 mg/ml. In vivo analysis for first and second weeks after treatment found 32.2% and 33% anthelminthic efficacy, respectively.
Instituto de Ciências Agrárias, Universidade Federal de Minas Gerais, Av Universitária 1000, Bairro Universitário, Montes Claros, Minas Gerais, 39400-006, Brazil.
Flávia A
FA
Nogueira
Leydiana D
LD
Fonseca
Rayana B
RB
da Silva
Adriano V
AV
de Paiva Ferreira
Patrícia S
PS
Nery
Luciana C
LC
Geraseev
Eduardo R
ER
Duarte
D000818:Animals; D000871:Anthelmintics / Q000008:administration & dosage / Q000009:adverse effects / Q000302:isolation & purification / Q000494:pharmacology*; D020448:Ericales / Q000737:chemistry*; D005243:Feces / Q000469:parasitology; D006373:Helminthiasis / Q000469:parasitology; D006374:Helminthiasis, Animal / Q000469:parasitology*; D007410:Intestinal Diseases / Q000469:parasitology / Q000662:veterinary*; D007411:Intestinal Diseases, Parasitic; D051379:Mice; D009348:Nematoda / Q000187:drug effects*; D009349:Nematode Infections / Q000469:parasitology*; D010270:Parasite Egg Count; D010936:Plant Extracts / Q000008:administration & dosage / Q000009:adverse effects / Q000302:isolation & purification / Q000494:pharmacology*; D012756:Sheep; D016019:Survival Analysis
D000871:Anthelmintics; D010936:Plant Extracts