An effective strategy for the synthesis of biocompatible gold nanoparticles using danshensu antioxidant: prevention of cytotoxicity via attenuation of free radical formation.
To suppress the cytotoxicity of gold nanoparticles (AuNPs), danshensu, a naturally occurring polyphenol antioxidant isolated from Chinese herb, was used to provide a fundamental protection layer for AuNPs, to alleviate oxidative stress and as a reducing agent to react with chloroauric acid. Besides danshensu, gum arabic was chosen as an auxiliary stabilising agent to improve the stability of AuNPs against aggregation. As expected, the prepared GA-DS-AuNPs (gum arabic-danshensu-gold nanoparticle) was remarkably stable in various buffer solutions. More interestingly, the GA-DS-AuNPs not only did not show any appreciable cytotoxicity, but also could alleviate the oxidative damage induced by AuNPs. Meanwhile, the ROS/RNS scavenging activities of GA-DS-AuNPs was evaluated by electron spin resonance spectroscopy (ESR), potentiometric nitric oxide (NO) sensor and cell confocal imaging. The results suggest that GA-DS-AuNPs might have effectively reduced the AuNPs-induced cytotoxicity and oxidative stress by downregulation of ROS/NOS production. The GA-DS-AuNPs may provide potential opportunities for the application in nanomedicine and nanobiology.
Institute of Chemistry, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Chinese Academy of Sciences, Beijing, PR China.
Libo
L
Du
Xiaoxiang
X
Miao
Yugang
Y
Jiang
Hongying
H
Jia
Qiu
Q
Tian
Jiangang
J
Shen
Yang
Y
Liu
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004355:Drug Stability; D005609:Free Radicals / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D006046:Gold / Q000494:pharmacology* / Q000633:toxicity; D042541:Intracellular Space / Q000378:metabolism; D007773:Lactates / Q000494:pharmacology*; D008315:Malondialdehyde / Q000032:analysis / Q000378:metabolism; D053768:Metal Nanoparticles / Q000008:administration & dosage* / Q000633:toxicity; D051379:Mice; D009569:Nitric Oxide / Q000032:analysis / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects
D000975:Antioxidants; D001672:Biocompatible Materials; D005609:Free Radicals; D007773:Lactates; D009569:Nitric Oxide; C035055:3,4-dihydroxyphenyllactic acid; D008315:Malondialdehyde; D006046:Gold