DNA interaction studies of a platinum(II) complex containing L-histidine and 1,10-phenanthroline ligands.
The aim of this study was developing coordination complexes that can be used as inorganic medicinal agents. The water soluble [Pt(phen)(His)]NO(3)·3H(2)O complex in which phen=1,10-phenantheroline and His=L-histidine was synthesized and characterized using physicochemical methods. Binding interaction of this complex with calf thymus (CT) DNA was investigated by emission, absorption, circular dichroism, and viscosity measurement techniques. Upon addition of CT-DNA, changes were observed in the characteristic ultraviolet-visible (UV-Vis) bands (hypochromism) of the complex. The complex binds to CT-DNA in an intercalative mode. The calculated binding constant, K(b), was 8 ± 0.2 × 10(4) M(-1). In addition, circular dichroism (CD) study showed that the phenanthroline ligand was inserted between the base pair stack of the double-helical structure of DNA. Also, the fluorescence spectral characteristics showed an increase in fluorescence intensity of the platinum complex in the presence of increasing amounts of DNA solution. The experimental results showed that the platinum complex binds to DNA via intercalative and hydrogen bonding mode.
Department of Chemistry, Faculty of Science, Razi University, Kermanshah, Iran. nahidshahabadi@yahoo.com
Nahid
N
Shahabadi
Leila
L
Nemati
D000042:Absorption; D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D056831:Coordination Complexes / Q000737:chemistry / Q000378:metabolism*; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D006639:Histidine / Q000737:chemistry / Q000378:metabolism*; D006860:Hydrogen Bonding; D008024:Ligands; D015394:Molecular Structure; D010618:Phenanthrolines / Q000737:chemistry / Q000378:metabolism*; D010984:Platinum / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics; D014783:Viscosity
D056831:Coordination Complexes; D008024:Ligands; D010618:Phenanthrolines; D010984:Platinum; D006639:Histidine; D004247:DNA; C427203:calf thymus DNA; C025205:1,10-phenanthroline