Characterization of Nipah virus from outbreaks in Bangladesh, 2008-2010.
Nipah virus (NiV) is a highly pathogenic paramyxovirus that causes fatal encephalitis in humans. The initial outbreak of NiV infection occurred in Malaysia and Singapore in 1998-1999; relatively small, sporadic outbreaks among humans have occurred in Bangladesh since 2001. We characterized the complete genomic sequences of identical NiV isolates from 2 patients in 2008 and partial genomic sequences of throat swab samples from 3 patients in 2010, all from Bangladesh. All sequences from patients in Bangladesh comprised a distinct genetic group. However, the detection of 3 genetically distinct sequences from patients in the districts of Faridpur and Gopalganj indicated multiple co-circulating lineages in a localized region over a short time (January-March 2010). Sequence comparisons between the open reading frames of all available NiV genes led us to propose a standardized protocol for genotyping NiV; this protcol provides a simple and accurate way to classify current and future NiV sequences.
Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA. mko2@cdc.gov
Michael K
MK
Lo
Luis
L
Lowe
Kimberly B
KB
Hummel
Hossain M S
HM
Sazzad
Emily S
ES
Gurley
M Jahangir
MJ
Hossain
Stephen P
SP
Luby
David M
DM
Miller
James A
JA
Comer
Pierre E
PE
Rollin
William J
WJ
Bellini
Paul A
PA
Rota
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001459:Bangladesh / Q000453:epidemiology; D002648:Child; D017124:Conserved Sequence; D004196:Disease Outbreaks; D005260:Female; D014644:Genetic Variation; D016679:Genome, Viral; D045464:Henipavirus Infections / Q000453:epidemiology* / Q000821:virology; D006801:Humans; D008969:Molecular Sequence Data; D058889:Molecular Typing; D045405:Nipah Virus / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D016036:Seroepidemiologic Studies; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
D014764:Viral Proteins