Visualizing neuromodulation in vivo: TANGO-mapping of dopamine signaling reveals appetite control of sugar sensing.
Behavior cannot be predicted from a "connectome" because the brain contains a chemical "map" of neuromodulation superimposed upon its synaptic connectivity map. Neuromodulation changes how neural circuits process information in different states, such as hunger or arousal. Here we describe a genetically based method to map, in an unbiased and brain-wide manner, sites of neuromodulation under different conditions in the Drosophila brain. This method, and genetic perturbations, reveal that the well-known effect of hunger to enhance behavioral sensitivity to sugar is mediated, at least in part, by the release of dopamine onto primary gustatory sensory neurons, which enhances sugar-evoked calcium influx. These data reinforce the concept that sensory neurons constitute an important locus for state-dependent gain control of behavior and introduce a methodology that can be extended to other neuromodulators and model organisms.
Howard Hughes Medical Institute, California Institute of Technology, Pasadena, CA 91125, USA.
Hidehiko K
HK
Inagaki
Shlomo
S
Ben-Tabou de-Leon
Allan M
AM
Wong
Smitha
S
Jagadish
Hiroshi
H
Ishimoto
Gilad
G
Barnea
Toshihiro
T
Kitamoto
Richard
R
Axel
David J
DJ
Anderson
D000818:Animals; D001069:Appetite Regulation; D019393:Arrestin / Q000378:metabolism; D001921:Brain / Q000502:physiology; D001931:Brain Mapping / Q000379:methods; D004298:Dopamine / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000502:physiology*; D005247:Feeding Behavior; D005260:Female; D018377:Neurotransmitter Agents / Q000378:metabolism*; D011954:Receptors, Dopamine / Q000378:metabolism; D011984:Sensory Receptor Cells / Q000378:metabolism; D015398:Signal Transduction
D019393:Arrestin; D018377:Neurotransmitter Agents; D011954:Receptors, Dopamine; D004298:Dopamine