Cloning whole bacterial genomes in yeast.
Many bacterial and archaeal genomes are of a similar size to molecules that have been cloned in the yeast Saccharomyces cerevisiae and thus might be clonable as single, circular episomes in this host. Yeast offers a variety of efficient tools for the manipulation and study of cloned DNA. One strategy to clone a genome in yeast is to cotransform yeast spheroplasts with the genome of interest and a linear yeast vector whose termini are homologous to a spot in the genome. Clones are selected on auxotrophic medium and then screened for completeness and size; they may also be sequenced.
J. Craig Venter Institute Inc., San Diego, CA, USA. gwynedd.benders@gmail.com
Gwynedd A
GA
Benders
D003001:Cloning, Molecular / Q000379:methods*; D046508:Culture Techniques; D004247:DNA / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D005818:Genetic Engineering; D005822:Genetic Vectors / Q000235:genetics; D016680:Genome, Bacterial / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics*; D013104:Spheroplasts / Q000235:genetics; D058615:Synthetic Biology; D014170:Transformation, Genetic
D004247:DNA; D004262:DNA Restriction Enzymes