Chemical composition of five wild edible mushrooms collected from Southwest China and their antihyperglycemic and antioxidant activity.
Evaluation of the chemical composition and antihyperglycemic and antioxidant activity of five wild edible mushrooms (Clitocybe maxima, Catathelasma ventricosum, Stropharia rugoso-annulata, Craterellus cornucopioides and Laccaria amethystea) from Southwest China. The chemical composition assay includes proximate analysis (moisture, ash, crude protein, crude fat, total carbohydrates and total energy), bioactive compounds analysis (total phenolic, flavonoid, ascorbic acid, ergosterol, tocopherol), fatty acid analysis, amino acid analysis, phenolic compounds analysis and mineral analysis of these mushrooms. Furthermore, assays of α-glucosidase inhibitory and α-amylase inhibitory activity were used for evaluating antihyperglycemic activity of the mushrooms, and assays of reducing power, chelating effect on ferrous ions, scavenging effect on hydroxyl free radicals and 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity were used for evaluating antioxidant activity of the mushrooms. Based on the results, ethanolic and aqueous extract of these mushroom all showed antihyperglycemic and antioxidant potential. In particular, the aqueous extract of C. ventricosum revealed the highest α-glucosidase inhibitory activity (EC50 value 2.74 μg/mL), DPPH radical scavenging activity (EC50 value 2.86 mg/mL) and reducing power (EC50 value 0.96 mg/mL), while the aqueous extract of L. amethystea showed the highest α-amylase inhibitory activity (EC50 value 4.37 μg/mL) and metal chelating activity (EC50 value 2.13 mg/mL).
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Yun-Tao
YT
Liu
Jun
J
Sun
Ze-Yu
ZY
Luo
Sheng-Qi
SQ
Rao
Yu-Jie
YJ
Su
Rong-Rong
RR
Xu
Yan-Jun
YJ
Yang
D000363:Agaricales / Q000737:chemistry* / Q000145:classification; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D002681:China; D002851:Chromatography, High Pressure Liquid; D004353:Drug Evaluation, Preclinical; D065089:Glycoside Hydrolase Inhibitors; D007004:Hypoglycemic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D013045:Species Specificity; D000516:alpha-Amylases / Q000037:antagonists & inhibitors
D000975:Antioxidants; D065089:Glycoside Hydrolase Inhibitors; D007004:Hypoglycemic Agents; D000516:alpha-Amylases