Immunological characterization of Mycoplasma hyopneumoniae recombinant proteins.
Mycoplasma hyopneumoniae, the primary pathogen of enzootic pneumonia, is highly prevalent worldwide and causes major economic losses to the pig industry. Commercial vaccines are widely used in the control of this disease, however, they provide only partial protection. The aim of this study was to evaluate 34 recombinant proteins of M. hyopneumoniae expressed in Escherichia coli. Antigenic and immunogenic properties of these proteins were analyzed. For this, the proteins were tested against hyperimmune and convalescent pig sera through ELISA and Western blot. Immunogenicity of the recombinant proteins was evaluated in BALB/c mice following intramuscular inoculation. Most antigens were able to induce a strong immune response and sera from inoculated mice were able to recognize native proteins by cell ELISA and Western blot. Several recombinant proteins were specifically recognized by convalescent pig sera, indicating they are expressed during infection. These data may help to develop more efficacious vaccines against M. hyopneumoniae.
Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, Brazil. simonesimionatto@ufgd.edu.br
Simone
S
Simionatto
Silvana B
SB
Marchioro
Vanessa
V
Galli
Clarice B
CB
Brum
Catia S
CS
Klein
Raquel
R
Rebelatto
Everton F
EF
Silva
Sibele
S
Borsuk
Fabricio R
FR
Conceição
Odir A
OA
Dellagostin
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D045705:Mycoplasma hyopneumoniae / Q000235:genetics / Q000276:immunology*; D045729:Pneumonia of Swine, Mycoplasmal / Q000276:immunology* / Q000517:prevention & control; D011994:Recombinant Proteins / Q000276:immunology; D013552:Swine
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D011994:Recombinant Proteins