Quantitative crystallinity determination for E1010, a novel carbapenem antibiotic, using differential scanning calorimetry.
OBJECTIVE
The objective of this study was to develop a quantitative crystallinity analysis method for the bulk drug of E1010 ((+)-(4R,5S,6S)-6-[(R)-1-hydroxyethyl]-3-[(2S,4S)-2-[(R)-1-hydroxy-1-[(R)-pyrrolidin-3 -yl]methyl]pyrrolidin-4-yl]thio-4-methyl-7-oxo-1-azabicyclo[3.2.0]hept-2-ene-2-carboxylic acid monohydrochloride), a novel carbapenem antibiotic.


METHODS
X-ray analyses, thermal analyses and hygroscopicity measurements were used to elucidate the crystal structure and the solid state properties. To develop a quantitative method for the crystallinity of E1010 bulk drug, the relationship between enthalpy change obtained by differential scanning calorimetry (DSC) and crystalline form ratio was investigated.


RESULTS
E1010 bulk drug was found to exist in a crystalline trihydrate formed in two layers, i.e. a layer of E1010 free form, and a layer consisting of chloride ions and water molecules. The thermal analysis showed an endothermic peak derived from dehydration with the loss of crystal lattices at around 100°C as an onset. The enthalpy change value for the endothermic peak correlated well with crystalline content in binary physical mixtures of the crystalline trihydrate and the amorphous form. In addition, for nine lots of the bulk drug, a positive correlation between the enthalpy change and chemical stability in the solid state was observed.


CONCLUSIONS
This quantitative analysis of crystallinity using DSC could be applicable for the quality control of the bulk drug to detect variability among manufacturing batches and to estimate the chemical stability of partially amorphous samples.
Physical Chemistry, Analytical Research, CMC Japan, Pharmaceutical Science & Technology Function Unit, Eisai Product Creation Systems, Eisai Co., Ltd, Tsukuba, Ibaraki, Japan. i-kushida@hhc.eisai.co.jp
Ikuo
I
Kushida
D000900:Anti-Bacterial Agents / Q000737:chemistry*; D002152:Calorimetry, Differential Scanning / Q000379:methods*; D015780:Carbapenems / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004355:Drug Stability; D017155:Wettability; D014961:X-Ray Diffraction
D000900:Anti-Bacterial Agents; D015780:Carbapenems; C572335:E1010