Determination of discriminatory power of genetic markers used for genotyping Giardia duodenalis.
Small subunit ribosomal DNA (SSU-rDNA), glutamate dehydrogenase (gdh), beta-giardin, triosephosphate isomerase (tpi), and elongation factor 1-alpha (ef1-alpha) genes are useful genetic markers for genotypic analysis of the intestinal protozoan, Giardia duodenalis (syn. G. lamblia, G. intestinalis), the cause of enteric disease in humans. To quantitatively compare the discriminatory power of these loci, 43 fecal samples were collected from central, northern and eastern Thailand and G. duodenalis specimens were analyzed using PCR-based genotyping and subcloning methods. Approximately equal prevalence of assemblage A (21) and B (22) were present among these populations. Analysis of Simpson's index and Wallace coefficient values from assemblage B isolates together with the data obtained from GenBank showed that the combination of two loci provides a higher discrimination power for subgenotyping G. duodenalis than using any single locus.
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Suradej
S
Siripattanapipong
Saovanee
S
Leelayoova
Mathirut
M
Mungthin
R C Andrew
RC
Thompson
Parima
P
Boontanom
Wilai
W
Saksirisamphant
Peerapan
P
Tan-Ariya
D016054:DNA, Protozoan / Q000235:genetics; D030561:Databases, Nucleic Acid; D005243:Feces / Q000469:parasitology; D017125:Genes, Protozoan; D005819:Genetic Markers; D005838:Genotype; D016829:Giardia lamblia / Q000235:genetics*; D005873:Giardiasis / Q000469:parasitology*; D006801:Humans; D058885:Multilocus Sequence Typing; D016133:Polymerase Chain Reaction; D013785:Thailand
D016054:DNA, Protozoan; D005819:Genetic Markers