Acquired tolerance to oxidative stress in Bifidobacterium longum 105-A via expression of a catalase gene.
For improvement of tolerance to oxidative stress in Bifidobacterium longum 105-A, we introduced the Bacillus subtilis catalase gene (katE) into it. The transformant showed catalase activity (39 U/mg crude protein) in the intracellular fraction, which increased survival by ∼100-fold after a 1-h exposure to 4.4 mM H(2)O(2), decreased de novo H(2)O(2) accumulation, and increased survival in aerated cultures by 10(5)-fold at 24 h. The protection level was better than that conferred by exogenously added catalase.
The United Graduate School of Agricultural Science, Gifu University, Yanagido, Gifu, Japan.
Jianlong
J
He
Kouta
K
Sakaguchi
Tohru
T
Suzuki
D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics; D001644:Bifidobacterium / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000502:physiology*; D002374:Catalase / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D006861:Hydrogen Peroxide / Q000378:metabolism; D050296:Microbial Viability / Q000187:drug effects; D016877:Oxidants / Q000378:metabolism; D018384:Oxidative Stress; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological
D016877:Oxidants; D011994:Recombinant Proteins; D006861:Hydrogen Peroxide; D002374:Catalase