Characterization of cellulolytic extract from Pycnoporus sanguineus PF-2 and its application in biomass saccharification.
The aim of this work was to evaluate the biochemical features of the white-rot fungi Pycnoporus sanguineus cellulolytic complex and its utilization to sugarcane bagasse hydrolysis. When cultivated under submerged fermentation using corn cobs as carbon source, P. sanguineus produced high FPase, endoglucanase, β-glucosidase, xylanase, mannanase, α-galactosidase, α-arabinofuranosidase, and polygalacturonase activities. Cellulase activities were characterized in relation to pH and temperature. β-Glucosidase and FPase activities were higher at 55 °C, pH 4.5, and endoglucanase activity was higher at 60 °C, in a pH range of 3.5-4.0. All cellulase activities were highly stable at 40 and 50 °C through 48 h of pre-incubation. Crude enzymatic extract from P. sanguineus was applied in a saccharification experiment using acid-treated and alkali-treated sugarcane bagasse as substrate, and the hydrolysis yields were compared to that obtained by a commercial cellulase preparation. Reducing sugar yields of 60.4% and 64.0% were reached when alkali-treated bagasse was hydrolyzed by P. sanguineus extract and commercial cellulase, respectively. Considering the glucose production, it was observed that P. sanguineus extract and commercial cellulase ensured yields of 22.6% and 36.5%, respectively. The saccharification of acid-treated bagasse was lower than that of alkali-treated bagasse regardless of the cellulolytic extract. The present work showed that P. sanguineus has a great potential as an enzyme producer for biomass saccharification.
Department of Biochemistry and Molecular Biology, Federal University of Viçosa, Viçosa, Minas Gerais, 36570-000, Brazil.
Daniel Luciano
DL
Falkoski
Valéria Monteze
VM
Guimarães
Maíra Nicolau
MN
de Almeida
Acelino Couto
AC
Alfenas
Jorge Luiz
JL
Colodette
Sebastião Tavares
ST
de Rezende
D018533:Biomass; D002244:Carbon / Q000737:chemistry; D002480:Cellulase / Q000737:chemistry; D044602:Cellulases / Q000737:chemistry*; D002482:Cellulose / Q000737:chemistry; D004789:Enzyme Activation; D057075:Enzyme Assays; D005285:Fermentation; D006026:Glycoside Hydrolases / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D011096:Polygalacturonase / Q000737:chemistry; D055494:Pycnoporus / Q000737:chemistry / Q000201:enzymology*; D031786:Saccharum / Q000737:chemistry; D013696:Temperature; D003313:Zea mays / Q000737:chemistry; D000519:alpha-Galactosidase / Q000737:chemistry; D001617:beta-Glucosidase / Q000737:chemistry
D002244:Carbon; D002482:Cellulose; C027433:bagasse; D044602:Cellulases; D006026:Glycoside Hydrolases; C023305:hemicellulase; D011096:Polygalacturonase; D001617:beta-Glucosidase; D000519:alpha-Galactosidase; D002480:Cellulase