Evaluation of a centrifuged double Y-shape microfluidic platform for simple continuous cell environment exchange.
We have demonstrated the efficacy of a microfluidic medium exchange method for single cells using passive centrifugal force of a rotating microfluidic-chip based platform. At the boundary of two laminar flows at the gathering area of two microfluidic pathways in a Y-shape, the cells were successfully transported from one laminar flow to the other, without mixing the two microfluidic mediums of the two laminar flows during cell transportation, within 5 s with 1 g (150 rpm) to 36.3 g (900 rpm) acceleration, with 93.5% efficiency. The results indicate that this is one of the most simple and precise tools for exchanging medium in the shortest amount of time.
Kanagawa Academy of Science and Technology, KSP East 310, 3-2-1 Sakado, Kawasaki 213-0012, Japan.
Akihiro
A
Hattori
Kanagawa Academy of Science and Technology, KSP East 310, 3-2-1 Sakado, Kawasaki 213-0012, Japan.|Department of Biomedical Information, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda, Tokyo 101-0062, Japan.
Kenji
K
Yasuda
D002021:Buffers; D002469:Cell Separation / Q000295:instrumentation*; D002498:Centrifugation; D004867:Equipment Design; D005456:Fluorescent Dyes / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*
D002021:Buffers; D005456:Fluorescent Dyes