Evaluation of new biomarker genes for differentiating Haemophilus influenzae from Haemophilus haemolyticus.
PCR detecting the protein D (hpd) and fuculose kinase (fucK) genes showed high sensitivity and specificity for identifying Haemophilus influenzae and differentiating it from H. haemolyticus. Phylogenetic analysis using the 16S rRNA gene demonstrated two distinct groups for H. influenzae and H. haemolyticus.
Meningitis and Vaccine Preventable Diseases Branch, Centers for Disease Control and Prevention (CDC), Atlanta, Georgia, USA. ale7@cdc.gov
M Jordan
MJ
Theodore
Raydel D
RD
Anderson
Xin
X
Wang
Lee S
LS
Katz
Jeni T
JT
Vuong
Melissa E
ME
Bell
Billie A
BA
Juni
Sara A
SA
Lowther
Ruth
R
Lynfield
Jessica R
JR
MacNeil
Leonard W
LW
Mayer
D001426:Bacterial Proteins / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics; D005819:Genetic Markers; D006193:Haemophilus influenzae / Q000235:genetics*; D007072:Immunoglobulin D / Q000235:genetics; D008074:Lipoproteins / Q000235:genetics; D058889:Molecular Typing; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D001426:Bacterial Proteins; D002352:Carrier Proteins; D005819:Genetic Markers; D007072:Immunoglobulin D; D008074:Lipoproteins; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D017853:Phosphotransferases (Alcohol Group Acceptor); C067989:glpQ protein, Haemophilus influenzae