Negative visualization of DNA in agarose gel by eosin Y and its related mechanism.
We report here a sensitive and environmentally benign technique for the visualization of DNA by using eosin Y, which appeared as a transparent and colorless band under the opaque gel matrix background. As low as 0.2-0.6 ng of DNA can be visualized within 1 h, similar to that of the most commonly used ethidium bromide stain. Furthermore, the mechanism of the newly developed method was also described. According to the results, it can be concluded that the complex between DNA and eosin Y is mainly formed by the function of groove binding, but not intercalate itself into the double helix structure of DNA.
Zhejiang Provincial Key Laboratory of Biopharmaceuticals, Wenzhou Medical College, Wenzhou, 325035, Zhejiang, PR China.
Weitao
W
Cong
Weijing
W
You
Mao
M
Chen
Jin
J
Ling
Zhongxin
Z
Zhu
Jungkap
J
Choi
Litai
L
Jin
Xiaokun
X
Li
D000818:Animals; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D004801:Eosine Yellowish-(YS) / Q000737:chemistry*; D004996:Ethidium / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D007364:Intercalating Agents / Q000737:chemistry; D013194:Staining and Labeling / Q000379:methods*
D005456:Fluorescent Dyes; D007364:Intercalating Agents; D004247:DNA; D004996:Ethidium; D004801:Eosine Yellowish-(YS)