A novel full-length isoform of murine pregnancy-specific glycoprotein 16 (psg16) is expressed in the brain but does not mediate murine coronavirus (MHV) entry.
The mouse pregnancy-specific glycoprotein 16 (PSG16) has been reported to be an alternative receptor for mouse hepatitis virus (MHV), some strains of which cause encephalitis in mice lacking the canonical receptor CEACAM1a. The known isoforms of PSG16 are N-terminally truncated relative to other PSG family proteins and are expressed in neurons as well as in the placenta. We have cloned a novel full-length isoform of psg16 that is also expressed in the brain, placenta, and retina but, like the truncated form, lacks MHV receptor activity when expressed on 293T cells, suggesting that PSG16 does not mediate CEACAM1a-independent spread of MHV.
Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, 203A Johnson Pavilion, 3610 Hamilton Walk, Philadelphia, PA 19104-6076, USA.
Judith M
JM
Phillips
I-Ting
IT
Kuo
Chelsea
C
Richardson
Susan R
SR
Weiss
D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000821:virology; D002272:Carcinoembryonic Antigen / Q000235:genetics / Q000378:metabolism*; D018352:Coronavirus Infections / Q000821:virology*; D005260:Female; D015870:Gene Expression; D057809:HEK293 Cells; D006524:Hepatitis, Viral, Animal / Q000821:virology*; D006801:Humans; D051379:Mice; D006517:Murine hepatitis virus / Q000502:physiology*; D009474:Neurons / Q000378:metabolism; D010920:Placenta / Q000378:metabolism; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000235:genetics / Q000378:metabolism*; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012160:Retina / Q000378:metabolism; D014162:Transfection; D053586:Virus Internalization
D002272:Carcinoembryonic Antigen; C509693:PSG16 protein, mouse; D011257:Pregnancy Proteins; D020033:Protein Isoforms