Study on the age-dependent tissue expression of FUT1 gene in porcine and its relationship to E. coli F18 receptor.
Escherichia coli (E. coli) that produces adhesin F18 is the main pathogen responsible for porcine post-weaning diarrhea and edema disease. The receptor for E. coli F18 has not been described in pigs, however the alpha (1,2)-fucosyltransferase (FUT1) gene on chromosome 6 has been proposed as a candidate. The objective of this study, therefore, was to investigate the relationship between FUT1 gene expression and E. coli F18 receptor in Sutai pigs of different ages (8-, 18-, 30- and 35-day-old). FUT1 gene expression was detected in 11 pig tissues with the highest level in lung, and expressed consistently at the four time points. In most tissues, FUT1 gene expression levels decreased from days 8 to 18, then continually increased on days 30 and 35, with expression around weaning time higher than that on day 8. Gene ontology and pathway analysis showed that FUT1 was involved in 32 biological processes, mainly those integral to the membrane, or involved in glycosylation, as well as regulation of binding, interestingly participating in three pathways related to glycosphingolipid biosynthesis. From this analysis and the high linkage disequilibrium between the FUT1 gene and the E. coli F18 receptor locus, we can speculate that higher expression of the FUT1 gene in small intestine is beneficial to the formation of receptors to the E. coli F18 strain and is related to the sensitivity to the pathogen.
College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Wen-Bin
WB
Bao
Lan
L
Ye
Chen
C
Zi
Xian-Min
XM
Su
Zhang-Yuan
ZY
Pan
Jin
J
Zhu
Guo-Qiang
GQ
Zhu
Xiao-Guo
XG
Huang
Sheng-Long
SL
Wu
D018829:Adhesins, Bacterial / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D000818:Animals; D027682:Cation Transport Proteins; D004926:Escherichia coli / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D004927:Escherichia coli Infections / Q000235:genetics* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D005647:Fucosyltransferases / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D056426:Genetic Loci; D006023:Glycoproteins; D015810:Linkage Disequilibrium; D029701:Saccharomyces cerevisiae Proteins; D013552:Swine
D018829:Adhesins, Bacterial; D027682:Cation Transport Proteins; D029968:Escherichia coli Proteins; C445317:FIT1 protein, S cerevisiae; D006023:Glycoproteins; D029701:Saccharomyces cerevisiae Proteins; D005647:Fucosyltransferases