Lipid-containing viruses: bacteriophage PRD1 assembly.
PRD1 is a tailless icosahedrally symmetric virus containing an internal lipid membrane beneath the protein capsid. Its linear dsDNA genome and covalently attached terminal proteins are delivered into the cell where replication occurs via a protein-primed mechanism. Extensive studies have been carried out to decipher the roles of the 37 viral proteins in PRD1 assembly, their association in virus particles and lately, especially the functioning of the unique packaging machinery that translocates the genome into the procapsid. These issues will be addressed in this chapter especially in the context of the structure of PRD1. We will also discuss the major challenges still to be addressed in PRD1 assembly.
Institute of Biotechnology, University of Helsinki, Helsinki, Finland. sarah.butcher@helsinki.fi
Sarah J
SJ
Butcher
Violeta
V
Manole
Nelli J
NJ
Karhu
D025622:Bacteriophage PRD1 / Q000737:chemistry* / Q000502:physiology*; D005814:Genes, Viral; D008055:Lipids / Q000737:chemistry*; D008958:Models, Molecular; D011487:Protein Conformation; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D014771:Virion / Q000378:metabolism / Q000648:ultrastructure; D014780:Viruses / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure
D008055:Lipids; D014764:Viral Proteins