Wolbachia heat shock protein 60 induces pro-inflammatory cytokines and apoptosis in monocytes in vitro.
Recombinant Wolbachia heat shock protein 60 (rWmhsp60) induces gene expression of pro-inflammatory cytokines IL-1β, IL-6 and TNF-α in human monocytic cell line THP-1. In addition, it inhibits the phagocytic activity and does not alter the nitric oxide production by differentiated THP-1 macrophages, which corroborates with no significant change in inducible nitric oxide synthase gene expression in rWmhsp60 treated THP-1 monocytes. Further, 24 h stimulation of peripheral blood mononuclear cells from normal individuals by rWmhsp60 reveals that monocytes enter the late apoptotic stage, while lymphocytes do not show apoptosis. Thus these findings suggest that rWmhsp60 may contribute to inflammation mediated monocyte dysfunction in filarial pathogenesis.
Centre for Biotechnology, Anna University, Chennai 600025, India.
Vijayan
V
Kamalakannan
Sreenivas
S
Kirthika
Kalyanaraman
K
Haripriya
Subash
S
Babu
Rangarajan Badri
RB
Narayanan
D017209:Apoptosis / Q000502:physiology*; D002460:Cell Line; D018834:Chaperonin 60 / Q000378:metabolism*; D016207:Cytokines / Q000096:biosynthesis*; D005368:Filariasis / Q000276:immunology / Q000382:microbiology; D005786:Gene Expression Regulation; D006801:Humans; D007249:Inflammation / Q000382:microbiology*; D008214:Lymphocytes / Q000276:immunology; D009000:Monocytes / Q000276:immunology*; D019001:Nitric Oxide Synthase / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism; D020577:Wolbachia / Q000378:metabolism*
D018834:Chaperonin 60; D016207:Cytokines; D011994:Recombinant Proteins; D019001:Nitric Oxide Synthase