Histomorphometric analysis of the effects of creatine on rat myometrium.
OBJECTIVE
To analyze the myometrial thickness of rats subjected to creatine (Cr) ingestion.


METHODS
A total of 14 rats was equally divided into the control group (ConGr) receiving 1 ml potable water and the creatine group (CrGr) subjected to the ingestion of 1.6 g/kg Cr diluted in 1 ml potable water. At the end of 8 weeks, the animals were anesthetized (xylazine and ketamine) and sacrificed, the uteri and ovaries stained with hematoxylin and eosin, the thickness of both the myometrium and the epithelium measured and the follicles counted.


RESULTS
Analysis revealed a significant increase in thickness of the myometrium in the CrGr (272.26 ± 66.71μm) contrasted with that from the ConGr (160.76 ± 35.65μm), CrGr > ConGr (p < 0001).


CONCLUSIONS
Our data suggest that Cr changed myometrial morphology in rats by enhancing myometrial thickness, but its action mechanism in the smooth muscle is still unclear.
Department of Obstetrics and Gynecology, Laboratory of Structural and Molecular Gynecology (LIM 58), School of Medicine - University of São Paulo, São Paulo, Brazil. lim58usp@gmail.com
Vinícius Cestari
VC
Do Amaral
Manuel De Jesus
Mde J
Simões
Rodrigo Rodrigues
RR
Marcondes
Jairo José
JJ
Matozinho Cubas
Edmund Chada
EC
Baracat
José Maria
JM
Soares
D000818:Animals; D003401:Creatine / Q000009:adverse effects* / Q000378:metabolism; D004031:Diestrus / Q000378:metabolism; D019587:Dietary Supplements / Q000009:adverse effects*; D004717:Endometrium / Q000166:cytology / Q000378:metabolism; D005260:Female; D006984:Hypertrophy; D009215:Myometrium / Q000378:metabolism / Q000473:pathology*; D009929:Organ Size; D010053:Ovary / Q000166:cytology / Q000378:metabolism; D058573:Performance-Enhancing Substances / Q000009:adverse effects* / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D058573:Performance-Enhancing Substances; D003401:Creatine