In vitro antioxidant activities of the polysaccharides from Tricholoma lobayense.
The antioxidant activities of three polysaccharide components (TLH-1, TLH-2, TLH-3) extracted from Tricholoma lobayense were evaluated by three different in vitro methods, namely superoxide radical (O(2)(-)) scavenging activity, inhibition of mice erythrocyte hemolysis (MEH) and malondialdehyde (MDA) mediated by hydrogen peroxide (H(2)O(2)) and investigation of oxidative modification of human serum albumin (HSA) induced by 2,2-azobis(2-amidinopropane)dihydrochloride (AAPH) through fluorescence spectroscopy. The antioxidant experiments showed that the polysaccharides had a notable activity in scavenging O(2)(-) in a concentration-dependent manner; H(2)O(2)-induced MEH and formation of MDA were effectively inhibited; by fluorescence spectroscopy, it was demonstrated that the polysaccharides could obviously inhibit AAPH-induced oxidative modification of HSA. The experimental data obtained from the in vitro models clearly revealed that TLH-3 had stronger antioxidant potency than TLH-1 and TLH-2, which indicated that TLH-3 might be exploited as effective natural antioxidant to alleviate oxidative stress.
School of Life Sciences, Anhui University, Hefei 230039, China.
Cui
C
Wang
Yan
Y
Chen
Meili
M
Hu
Jingna
J
Ding
Cunji
C
Xu
Ruijun
R
Wang
D000818:Animals; D004912:Erythrocytes / Q000187:drug effects; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D008315:Malondialdehyde / Q000037:antagonists & inhibitors; D051379:Mice; D010084:Oxidation-Reduction; D011134:Polysaccharides / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D012709:Serum Albumin / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013481:Superoxides / Q000378:metabolism; D055439:Tricholoma / Q000737:chemistry*
D016166:Free Radical Scavengers; D011134:Polysaccharides; D012709:Serum Albumin; D013481:Superoxides; D008315:Malondialdehyde