Aptamer-based viability impedimetric sensor for viruses.
The development of aptamer-based viability impedimetric sensor for viruses (AptaVISens-V) is presented. Highly specific DNA aptamers to intact vaccinia virus were selected using cell-SELEX technique and integrated into impedimetric sensors via self-assembly onto a gold microelectrode. Remarkably, this aptasensor is highly selective and can successfully detect viable vaccinia virus particles (down to 60 virions in a microliter) and distinguish them from nonviable viruses in a label-free electrochemical assay format. It also opens a new venue for the development of a variety of viability sensors for detection of many microorganisms and spores.
Department of Chemistry, University of Ottawa, 10 Marie Curie, Ottawa, Ontario K1N 6N5, Canada.
Mahmoud
M
Labib
Anna S
AS
Zamay
Darija
D
Muharemagic
Alexey V
AV
Chechik
John C
JC
Bell
Maxim V
MV
Berezovski
D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000235:genetics*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008839:Microelectrodes; D014780:Viruses / Q000378:metabolism*
D052157:Aptamers, Nucleotide; D006046:Gold