Phagemid vectors for phage display: properties, characteristics and construction.
Phagemids are filamentous-phage-derived vectors containing the replication origin of a plasmid. Phagemids usually encode no or only one kind of coat proteins. Other structural and functional proteins necessary to accomplish the life cycle of phagemid are provided by the helper phage. In addition, other elements such as molecular tags and selective markers are introduced into the phagemids to facilitate the subsequent operations, such as gene manipulation and protein purification. This review summarizes the elements of the phagemids and their corresponding functions. Finally, the possible trends and future direction to improve the characteristics of the phagemids are highlighted.
Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China.
Huan
H
Qi
Haiqin
H
Lu
Hua-Ji
HJ
Qiu
Valery
V
Petrenko
Aihua
A
Liu
D000595:Amino Acid Sequence; D001435:Bacteriophages / Q000235:genetics*; D036022:Capsid Proteins / Q000235:genetics; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes / Q000378:metabolism; D005818:Genetic Engineering / Q000379:methods* / Q000639:trends; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D019151:Peptide Library; D010957:Plasmids / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D021382:Protein Sorting Signals; D018741:Replication Origin / Q000235:genetics
D036022:Capsid Proteins; D019151:Peptide Library; D021382:Protein Sorting Signals; D004262:DNA Restriction Enzymes