Crystallization and X-ray diffraction analysis of chondroitin lyase from baculovirus: envelope protein ODV-E66.
Baculovirus envelope protein ODV-E66 (67-704), in which the N-terminal 66 amino acids are truncated, is a chondroitin lyase. It digests chondroitin and chondroitin 6-sulfate efficiently, but does not digest chondroitin 4-sulfate. This unique characteristic is useful for the preparation of specific chondroitin oligosaccharides and for investigation of the mechanism of baculovirus infection. ODV-E66 (67-704) was crystallized; the crystal diffracted to 1.8 Å resolution and belonged to space group P6(2) or P6(4), with unit-cell parameters a = b = 113.5, c = 101.5 Å. One molecule is assumed to be present per asymmetric unit, which gives a Matthews coefficient of 2.54 Å(3) Da(-1).
Laboratory of Structural Biology, Graduate School of System Life Sciences, Kyushu University, Fukuoka City, Fukuoka, Japan.
Yoshirou
Y
Kawaguchi
Nobuo
N
Sugiura
Momo
M
Onishi
Koji
K
Kimata
Makoto
M
Kimura
Yoshimitu
Y
Kakuta
D016367:Baculoviridae / Q000201:enzymology*; D002808:Chondroitin Lyases / Q000737:chemistry*; D003460:Crystallization; D015686:Gene Products, env / Q000737:chemistry*; D014961:X-Ray Diffraction
D015686:Gene Products, env; D002808:Chondroitin Lyases