Assay and characterization of an osmolarity inducible promoter newly isolated from Bacillus subtilis.
An osmolarity-sensitive promoter fragment, P23423, isolated from Bacillus subtilis was characterized. The expression of β-galactosidase (β-Gal) driven by P23423 was regulated by osmolarity both in Escherichia coli and B. subtilis. The classical conserved region of this prokaryotic promoter was found within the sequence of the cloned fragment, and the putative promoter was identified as the control element of RNA not coding for protein (a RNA molecule that is not translated into a protein). The efficiency and benefit of this promoter was further demonstrated via osmolarity-induced expression of three other heterologous proteins in E. coli. Thus, this approach provided a simple and inexpensive inducible promoter element for the expression of cloned genes.
College of Life Sciences, Northwest A&F University, Yangling 712100, People's Republic of China.
Wei-Wei
WW
Zhang
Qiu-Rong
QR
Gao
Ming-Ming
MM
Yang
Hui
H
Liu
Dun
D
Wang
D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D004926:Escherichia coli / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009994:Osmolar Concentration; D011401:Promoter Regions, Genetic; D022661:RNA, Untranslated; D017422:Sequence Analysis, DNA; D015534:Trans-Activators / Q000235:genetics*; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics*
D001426:Bacterial Proteins; D022661:RNA, Untranslated; D015534:Trans-Activators; C112177:osmolarity response regulator proteins; D001616:beta-Galactosidase