How to conduct and interpret ITC experiments accurately for cyclodextrin-guest interactions.
Isothermal titration calorimetry (ITC) is one of the most interesting methods for the characterization of the interaction mechanisms of cyclodextrins (CDs) with drugs. In this review we explain how to conduct ITC experiments correctly for CD-guest interactions, how to choose an accurate fitting model for the titration curve and how to interpret carefully the ITC results. Finally, the use of ITC for the characterization of CD-containing nanoparticles is discussed.
Université Paris-Sud, School of Pharmacy, UMR CNRS, Châtenay Malabry, France. Kawthar.bouchemal@u-psud.fr
Kawthar
K
Bouchemal
Silvia
S
Mazzaferro
D002151:Calorimetry / Q000379:methods* / Q000592:standards; D003505:Cyclodextrins / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D004347:Drug Interactions; D008956:Models, Chemical; D015394:Molecular Structure; D053758:Nanoparticles; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D013499:Surface Properties; D013816:Thermodynamics; D017186:Titrimetry
D003505:Cyclodextrins; D004337:Drug Carriers; D004364:Pharmaceutical Preparations