Library-free methylation sequencing with bisulfite padlock probes.
Targeted quantification of DNA methylation allows for interrogation of the most informative loci across many samples quickly and cost-effectively. Here we report improved bisulfite padlock probes (BSPPs) with a design algorithm to generate efficient padlock probes, a library-free protocol that dramatically reduces sample-preparation cost and time and is compatible with automation, and an efficient bioinformatics pipeline to accurately obtain both methylation levels and genotypes from sequencing of bisulfite-converted DNA.
Department of Bioengineering, University of California at San Diego, La Jolla, California, USA.
Dinh
D
Diep
Nongluk
N
Plongthongkum
Athurva
A
Gore
Ho-Lim
HL
Fung
Robert
R
Shoemaker
Kun
K
Zhang
D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000235:genetics*; D015342:DNA Probes / Q000737:chemistry* / Q000235:genetics*; D015723:Gene Library; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*; D013447:Sulfites / Q000737:chemistry*
D015342:DNA Probes; D013447:Sulfites; D004247:DNA; C042345:hydrogen sulfite