Degradation of actin nucleators affects cortical polarity of aged mouse oocytes.
OBJECTIVE
To investigate the molecular mechanism of mouse oocyte polarity loss during aging.


METHODS
Experimental study.


METHODS
Academic basic research laboratory.


METHODS
Mice.


METHODS
Oocytes were collected 16 hours after injection of hCG and cultured in M16 medium for an additional 14 hours with or without caffeine.


METHODS
Expression and localizations of actin nucleators actin-related protein 2/3 complex, JMY, and WAVE2 were examined by immunofluorescence staining, and their messenger RNA levels were examined by real-time reverse transcription-polymerase chain reaction.


RESULTS
The protein and messenger RNA levels of actin-related protein 2/3 complex, JMY, and WAVE2 were decreased in aged oocytes, but the levels were normal in caffeine-treated aged oocytes.


CONCLUSIONS
Our data indicated that the loss of oocyte polarity may be due to the degradation of actin nucleators in aged oocytes.
Department of Animal Sciences, Chungbuk National University, Cheongju, Chungbuk, South Korea.
Shao-Chen
SC
Sun
Wei-Wei
WW
Gao
Yong-Nan
YN
Xu
Yong-Xun
YX
Jin
Qing-Ling
QL
Wang
Xi-Jun
XJ
Yin
Xiang-Shun
XS
Cui
Nam-Hyung
NH
Kim
D051376:Actin-Related Protein 2-3 Complex / Q000235:genetics / Q000378:metabolism*; D000199:Actins / Q000378:metabolism*; D000818:Animals; D002110:Caffeine / Q000494:pharmacology; D018797:Cell Cycle Proteins; D016764:Cell Polarity / Q000187:drug effects; D016922:Cellular Senescence; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D005260:Female; D005300:Fertility Agents, Female / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D051379:Mice; D008813:Mice, Inbred ICR; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D009865:Oocytes / Q000187:drug effects / Q000378:metabolism*; D010062:Ovulation Induction / Q000379:methods; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D051300:Wiskott-Aldrich Syndrome Protein Family / Q000235:genetics / Q000378:metabolism*
D051376:Actin-Related Protein 2-3 Complex; D000199:Actins; D018797:Cell Cycle Proteins; D006063:Chorionic Gonadotropin; D005300:Fertility Agents, Female; C485677:Jmy protein, mouse; D009687:Nuclear Proteins; D012333:RNA, Messenger; D015534:Trans-Activators; C492303:Wasf2 protein, mouse; D051300:Wiskott-Aldrich Syndrome Protein Family; D002110:Caffeine