A novel, highly sensitive and rapid allele-specific loop-mediated amplification assay for the detection of the JAK2V617F mutation in chronic myeloproliferative neoplasms.
BACKGROUND
The identification of the JAK2V617F mutation is mandatory in the diagnostic work-up of Philadelphia chromosome-negative myeloproliferative neoplasms. Several molecular techniques to detect this mutation are currently available, but each of them has some limits.


METHODS
We set up a novel molecular method for the identification of the JAK2V617F mutation based on an allele-specific loop-mediated amplification, not polymerase chain reaction analysis. This innovative technique amplifies DNA targets under isothermal conditions with high specificity, efficiency and rapidity. The method does not require either a thermal cycler or gel separation and the DNA amplification reaction is visible to the naked eye and can be monitored by turbidimetry. This method was validated on DNA from cell lines as well as from patients with myeloproliferative neoplasms. The results were compared with those obtained by conventional polymerase chain reaction methods.


RESULTS
This assay detects, within 1 hour, the JAK2V617F mutation down to an allele burden of 0.1-0.01%. All samples positive by polymerase chain reaction (n=146) proved positive when tested by allele-specific loop-mediated amplification and none of the 80 negative controls gave false positive results. In addition, six patients with essential thrombocythemia previously diagnosed as being JAK2V617F negative by polymerase chain reaction analysis were found to be positive (at a low level) by allele-specific loop-mediated amplification. Furthermore, this assay discriminated the amount of JAK2V617F tumor allele within intervals of positivity, above 50%, between 50% and 10% and below 10%.


CONCLUSIONS
Allele-specific loop-mediated amplification is a simple, robust and easily applicable method for the molecular diagnosis and monitoring of JAK2V617F mutation in patients with chronic myeloproliferative neoplasms.
Department of Biotechnology, University of Milano Bicocca, Italy.
Giulia
G
Minnucci
Giulia
G
Amicarelli
Silvia
S
Salmoiraghi
Orietta
O
Spinelli
Marie Lorena
ML
Guinea Montalvo
Ursula
U
Giussani
Daniel
D
Adlerstein
Alessandro
A
Rambaldi
D000483:Alleles; D001681:Biological Assay; D016022:Case-Control Studies; D002908:Chronic Disease; D004247:DNA / Q000032:analysis* / Q000235:genetics; D006801:Humans; D053614:Janus Kinase 2 / Q000235:genetics*; D009154:Mutation / Q000235:genetics*; D009196:Myeloproliferative Disorders / Q000175:diagnosis* / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D014407:Tumor Cells, Cultured
D004247:DNA; C507924:JAK2 protein, human; D053614:Janus Kinase 2