Identification and possible role of a MYB transcription factor from saffron (Crocus sativus).
The MYB family is the most abundant group of transcription factors described for plants. Plant MYB genes have been shown to be involved in the regulation of many aspects of plant development. No MYB genes are described for saffron, the dried stigma of Crocus sativus, utilized as a colorant for foodstuffs. In this study, we used RACE-PCR to isolate a full length cDNA of 894bp with a 591bp open reading frame, encoding a putative CsMYB1 from C. sativus. Comparison between gDNA and cDNA revealed no introns. Homology studies indicated that the deduced amino acid sequence is similar to members of the R2R3 MYB subfamily. Expression analysis showed the presence of high transcript levels in stigma tissue and low levels in tepals, whereas no signal was detected in either anthers or leaves. The RT-PCR analysis revealed that CsMYB1 expression is developmentally regulated during stigma development. Furthermore, expression analysis in stigmas from different Crocus species showed a correlation with stigma morphology. No transcripts were found in stigma tissues of Crocus species characterized by branched stigma morphology. Taken together, these results suggest that CsMYB1 may be involved in the regulation of stigma morphology in Crocus.
Departamento de Ciencia y Tecnología Agroforestal y Genética, Instituto Botánico, ETSIA, Universidad de Castilla-La Mancha, Campus Universitario s/n, Albacete 02071, Spain.
Lourdes
L
Gómez-Gómez
Almudena
A
Trapero-Mozos
Maria Dolores
MD
Gómez
Angela
A
Rubio-Moraga
Oussama
O
Ahrazem
D000595:Amino Acid Sequence; D027622:Crocus / Q000235:genetics* / Q000378:metabolism*; D035264:Flowers; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008969:Molecular Sequence Data; D010802:Phylogeny; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*
D014157:Transcription Factors