A multiwell electrochemical biosensor for real-time monitoring of the behavioural changes of cells in vitro.
We report the development of a multiwell biosensor for detecting changes in the electrochemical open circuit potential (OCP) generated by viable human cells in vitro. The instrument features eight culture wells; each containing three gold sensors around a common silver/silver chloride reference electrode, prepared using screen-printed conductive inks. The potential applications of the device were demonstrated by monitoring rheumatoid synovial fibroblasts (RSF) and HepG2 hepatocarcinoma cells in response to chemical and biological treatments. This technology could provide an alternative to conventional end-point assays used in the fields of chemotherapy, toxicology and drug discovery.
University Department Medicine, University of Manchester, MRI, Oxford Rd, Manchester, M13 9WL, UK. daman.adlam@manchester.ac.uk
Daman J
DJ
Adlam
David E
DE
Woolley
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D055808:Drug Discovery; D004563:Electrochemistry; D004566:Electrodes; D056945:Hep G2 Cells; D006801:Humans