α-Tocopherol sensitizes human leukemia HL-60 cells to apoptosis induced by benzyl isothiocyanate.
Pre-treatment with α-tocopherol (α-Toc) potentiated cytotoxicity induction by benzyl isothiocyanate (BITC). Biochemical events related to apoptosis, such as DNA ladder formation and caspase-3 activation, were also enhanced by α-Toc. These results suggest a significant role of the caspase-3 pathway in apoptosis induction regulated by α-Toc in combination.
Graduate School of Natural Science and Technology, Okayama University.
Naomi
N
Abe
Tomomi
T
Shimizu
Noriyuki
N
Miyoshi
Yoshiyuki
Y
Murata
Yoshimasa
Y
Nakamura
D000975:Antioxidants; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D053938:DNA Fragmentation; D004357:Drug Synergism; D018922:HL-60 Cells; D006801:Humans; D017879:Isothiocyanates / Q000494:pharmacology*; D024502:alpha-Tocopherol / Q000494:pharmacology* / Q000627:therapeutic use
D000975:Antioxidants; D017879:Isothiocyanates; C031403:benzyl isothiocyanate; D053148:Caspase 3; D024502:alpha-Tocopherol