Metabolic profiling and biological activities of bioactive compounds produced by Pseudomonas sp. strain ICTB-745 isolated from Ladakh, India.
In an ongoing survey of the bioactive potential of microorganisms from Ladakh, India, the culture medium of a bacterial strain of a new Pseudomonas sp., strain ICTB-745, isolated from an alkaline soil sample collected from Leh, Ladakh, India, was found to contain metabolites that exhibited broad-spectrum antimicrobial and biosurfactant activities. Bioactivity-guided purification resulted in the isolation of four bioactive compounds. Their chemical structures were elucidated by (1)H and (13)C NMR, 2D-NMR (HMBC, HSQC, (1)H,(1)H-COSY, and DEPT- 135), FT-IR, and mass spectroscopic methods, and were identified as 1-hydroxyphenazine, phenazine-1-carboxylic acid (PCA), rhamnolipid-1 (RL-1), and rhamnolipid-2 (RL-2). These metabolites exhibited various biological activities like antimicrobial and efficient cytotoxic potencies against different human tumor cell lines such as HeLa, HepG2, A549, and MDA MB 231. RL-1 and RL-2 exhibited a dose-dependent antifeedant activity against Spodoptera litura, producing about 82.06% and 73.66% antifeedant activity, whereas PCA showed a moderate antifeedant activity (63.67%) at 60 microgram/cm2 area of castor leaf. Furthermore, PCA, RL-1, and RL-2 exhibited about 65%, 52%, and 47% mortality, respectively, against Rhyzopertha dominica at 20 microgram/ml. This is the first report of rhamnolipids as antifeedant metabolites against Spodoptera litura and as insecticidal metabolites against Rhyzopertha dominica. The metabolites from Pseudomonas sp. strain ICTB-745 have interesting potential for use as a biopesticide in pest control programs.
Chemical Biology Laboratory, Division of Organic Chemistry, IndianInstitute of Chemical Technology, Uppal Road, Hyderabad 500 607, India. ahmedkamal@iict.res.in
Ahmed
A
Kamal
Anver Basha
AB
Shaik
C Ganesh
CG
Kumar
Poornima
P
Mongolla
P Usha
PU
Rani
K V S Rama
KV
Krishna
Suman Kumar
SK
Mamidyala
Joveeta
J
Joseph
D000890:Anti-Infective Agents / Q000737:chemistry / Q000302:isolation & purification; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification; D001688:Biological Products / Q000737:chemistry / Q000302:isolation & purification; D045744:Cell Line, Tumor; D003470:Culture Media / Q000737:chemistry; D006801:Humans; D007194:India; D007306:Insecticides / Q000737:chemistry / Q000302:isolation & purification; D055442:Metabolome; D011549:Pseudomonas / Q000302:isolation & purification* / Q000378:metabolism*; D013057:Spectrum Analysis; D013501:Surface-Active Agents / Q000737:chemistry / Q000302:isolation & purification; D016019:Survival Analysis
D000890:Anti-Infective Agents; D000970:Antineoplastic Agents; D001688:Biological Products; D003470:Culture Media; D007306:Insecticides; D013501:Surface-Active Agents