Contribution of amoebic coculture to recovery of Legionella isolates from respiratory samples: prospective analysis over a period of 32 months.
We evaluated the contribution of amoebic coculture to the recovery of Legionella spp. from 379 respiratory samples. The sensitivity of axenic culture was 42.1%. The combination of axenic culture with amoebic coculture increased the Legionella isolation rate to 47.1%. Amoebic coculture was particularly efficient in isolating Legionella spp. from respiratory samples contaminated with oropharyngeal flora.
Université de Lyon, Lyon, France. ghislaine.descours@univ-lyon1.fr
G
G
Descours
A
A
Suet
C
C
Ginevra
C
C
Campese
S
S
Slimani
F
F
Ader
D
D
Che
G
G
Lina
S
S
Jarraud
D000048:Acanthamoeba / Q000254:growth & development*; D001431:Bacteriological Techniques / Q000379:methods*; D006801:Humans; D007875:Legionella / Q000254:growth & development* / Q000302:isolation & purification*; D007876:Legionellosis / Q000175:diagnosis*; D011446:Prospective Studies; D012137:Respiratory System / Q000382:microbiology; D012680:Sensitivity and Specificity