Single-cell microinjection technologies.
Single-cell microinjection, as a mechanical delivery tool, has been used for transferring substances into transfection or infection challenging cells. Here, we discuss the advantages and applications of microinjection, list the materials needed for performing microinjection experiments, and describe the methods of single-cell microinjection into suspended and attached cells.
Laboratory of Neurobiology and State Key Laboratory of Biomembrane and Membrane Biotechnology, School of Life Sciences, Peking University, Beijing, China. yanzhang@pku.edu.cn
Yan
Y
Zhang
D000818:Animals; D002448:Cell Adhesion; D006801:Humans; D008845:Microinjections / Q000379:methods*; D008853:Microscopy; D059010:Single-Cell Analysis / Q000379:methods*