Nerium oleander indirect leaf photosynthesis and light harvesting reductions after clipping injury or Spodoptera eridania herbivory: high sensitivity to injury.
Variable indirect photosynthetic rate (P(n)) responses occur on injured leaves after insect herbivory. It is important to understand factors that influence indirect P(n) reductions after injury. The current study examines the relationship between gas exchange and chlorophyll a fluorescence parameters with injury intensity (% single leaf tissue removal) from clipping or Spodoptera eridania Stoll (Noctuidae) herbivory on Nerium oleander L. (Apocynaceae). Two experiments showed intercellular [CO(2)] increases but P(n) and stomatal conductance reductions with increasing injury intensity, suggesting non-stomatal P(n) limitation. Also, P(n) recovery was incomplete at 3d post-injury. This is the first report of a negative exponential P(n) impairment function with leaf injury intensity to suggest high N. oleander leaf sensitivity to indirect P(n) impairment. Negative linear functions occurred between most other gas exchange and chlorophyll a fluorescence parameters with injury intensity. The degree of light harvesting impairment increased with injury intensity via lower (1) photochemical efficiency indicated lower energy transfer efficiency from reaction centers to PSII, (2) photochemical quenching indicated reaction center closure, and (3) electron transport rates indicated less energy traveling through PSII. Future studies can examine additional mechanisms (mesophyll conductance, carbon fixation, and cardenolide induction) to cause N. oleander indirect leaf P(n) reductions after injury.
Department of Biology, Xavier University of Louisiana, New Orleans, LA 70125, United States. kevin.delaney@ars.usda.gov
Kevin J
KJ
Delaney
D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D000077194:Chlorophyll A; D004579:Electron Transport; D005453:Fluorescence; D060434:Herbivory; D008027:Light; D045342:Light-Harvesting Protein Complexes / Q000378:metabolism*; D030007:Nerium / Q000502:physiology* / Q000528:radiation effects; D010788:Photosynthesis / Q000502:physiology*; D045332:Photosystem II Protein Complex / Q000378:metabolism; D018515:Plant Leaves / Q000502:physiology / Q000528:radiation effects; D054046:Plant Stomata / Q000502:physiology; D018526:Plant Transpiration / Q000502:physiology; D018411:Spodoptera / Q000502:physiology*; D014947:Wounds and Injuries
D045342:Light-Harvesting Protein Complexes; D045332:Photosystem II Protein Complex; D002734:Chlorophyll; D002245:Carbon Dioxide; D000077194:Chlorophyll A