Motorcycle exhaust particles up-regulate expression of vascular adhesion molecule-1 and intercellular adhesion molecule-1 in human umbilical vein endothelial cells.
Epidemiological studies have shown that there is a strong correlation between atherosclerosis and ambient air pollution. In this study, we found that motorcycle exhaust particles (MEP) induced adhesion between cells of the human monocytic leukemia cell line (THP-1) and human umbilical vein endothelial cells (HUVECs) in a time-and dose-dependent manner. In addition, MEP treatment induced both mRNA and protein expression of vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) in HUVECs. The IκB degradation and p65 nuclear translocation was found in MEP-treated HUVECs, suggested the involvement of nuclear factor-κB (NF-κB). MEP-induced VCAM-1 and ICAM-1 protein expression was inhibited by NF-κB inhibitor BAY 11-7085. Oxidative stress was also involved in the signaling of VCAM-1 and ICAM-1 expression. MEP treatment caused hydrogen peroxide and superoxide formation. Pretreatment with α-tocopherol could inhibit MEP-induced reactive oxygen intermediates generation and suppressed MEP-induced IκB degradation and adhesion molecules expression. Furthermore, the carbon black (CB) nanoparticles with different diameters could induce VCAM-1 and ICAM-1 protein expression; however, polycyclic aromatic hydrocarbons (PAHs) only increased the expression of ICAM-1 but not that of VCAM-1 in HUVECs. In this study, we found that MEPs could induce ICAM-1 and VCAM-1 expression through oxidative stress and NF-κB activation in HUVECs.
Department of Microbiology and Immunology, School of Medicine, China Medical University, Taichung, Taiwan.
Chen-Chen
CC
Lee
Shih-Hsuan
SH
Huang
Ya-Ting
YT
Yang
Yu-Wen
YW
Cheng
Ching-Hao
CH
Li
Jaw-Jou
JJ
Kang
D000393:Air Pollutants / Q000633:toxicity*; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis* / Q000235:genetics; D009049:Motorcycles; D016328:NF-kappa B / Q000378:metabolism; D018384:Oxidative Stress; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity; D012333:RNA, Messenger / Q000096:biosynthesis; D053260:Soot / Q000633:toxicity; D015854:Up-Regulation; D019010:Vascular Cell Adhesion Molecule-1 / Q000096:biosynthesis* / Q000235:genetics; D001335:Vehicle Emissions / Q000633:toxicity*
D000393:Air Pollutants; D016328:NF-kappa B; D011084:Polycyclic Aromatic Hydrocarbons; D012333:RNA, Messenger; D053260:Soot; D019010:Vascular Cell Adhesion Molecule-1; D001335:Vehicle Emissions; D018799:Intercellular Adhesion Molecule-1