Two-step efficient synthesis of 5-methyluridine via two thermostable nucleoside phosphorylase from Aeropyrum pernix.
5-Methyluridine has been synthesized in high yield using guanosine and thymine as starting materials in the presence of highly thermostable recombinant purine nucleoside phosphorylase (PNP) and uridine phosphorylase (UP) obtained from hyperthermophilic aerobic crenarchaeon Aeropyrum pernix. Key reaction parameters such as pH, temperature, concentration of buffer and substrates were investigated. At the optimal conditions, 5-methyluridine was achieved in yield 85% with a guanosine conversion of 96% in 10ml scale. The process can be performed at high temperature, which will highly increase the solubility of substrates, therefore, this process is suitable for the industry application.
State Key Laboratory of Chemical Resources Engineering, Beijing University of Chemical Technology, Beijing 100029, People's Republic of China.
Shaozhou
S
Zhu
Lu
L
Ren
Jianjun
J
Wang
Guojun
G
Zheng
Pingwah
P
Tang
D041522:Aeropyrum / Q000201:enzymology* / Q000378:metabolism; D004795:Enzyme Stability; D006151:Guanosine / Q000378:metabolism; D007218:Industrial Microbiology; D010430:Pentosyltransferases / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D013696:Temperature; D013941:Thymine / Q000378:metabolism; D014529:Uridine / Q000031:analogs & derivatives* / Q000378:metabolism; D014543:Uridine Phosphorylase / Q000378:metabolism
D011994:Recombinant Proteins; D006151:Guanosine; C009182:ribothymidine; D010430:Pentosyltransferases; C067995:nucleoside phosphorylase; D014543:Uridine Phosphorylase; D013941:Thymine; D014529:Uridine