Asanoa siamensis sp. nov., isolated from soil from a temperate peat swamp forest.
A novel actinomycete strain, PS7-2(T), which produced spore chains borne on the tips of short sporophores, was isolated from soil collected from a temperate peat swamp forest in Phu-Sang National Park, Phayao province, Thailand. The isolate contained glutamic acid, glycine, alanine, 3-hydroxy-diaminopimelic acid and meso-diaminopimelic acid in the cell-wall peptidoglycan. The whole-cell sugars were glucose, mannose, rhamnose and xylose, and the major phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylethanolamine. The predominant menaquinones were MK-10(H(8)) and MK-9(H(8)) and the predominant cellular fatty acids were iso-C(15 : 0), anteiso-C(15 : 0) and anteiso-C(17 : 0). The G+C content of the genomic DNA was 72.3 mol%. On the basis of 16S rRNA gene sequence analysis, strain PS7-2(T) clustered with members of the genus Asanoa and appeared most closely related to the type strains of Asanoa hainanensis (99.5 % sequence similarity), Asanoa iriomotensis (99.0 %), Asanoa ishikariensis (98.9 %) and Asanoa ferruginea (98.5 %). DNA-DNA hybridizations and some physiological and biochemical properties indicated that strain PS7-2(T) could be readily distinguished from its closest phylogenetic relatives. Based on the phenotypic and genotypic evidence and DNA-DNA relatedness values, strain PS7-2(T) represents a novel species in the genus Asanoa, for which the name Asanoa siamensis sp. nov. is proposed; the type strain is PS7-2(T) (=BCC 41921(T) =NBRC 107932(T)).
Department of Biology, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Chalongkrung Road, Ladkrabang, Bangkok 10520, Thailand.
Nantawan
N
Niemhom
BIOTEC Culture Collection, Bioresources Technology Unit, National Centre for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Phaholyothin Road, Klong 1, Klong Luang, Pathumthani 12120, Thailand.
Chanwit
C
Suriyachadkun
Biological Resource Centre, National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0812, Japan.
Tomohiko
T
Tamura
Microbial Resource Management Unit, Scientific Instrument Centre, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Chalongkrung Road, Ladkrabang, Bangkok 10520, Thailand.|Department of Biology, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Chalongkrung Road, Ladkrabang, Bangkok 10520, Thailand.
Chitti
C
Thawai
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008848:Micromonosporaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013785:Thailand; D014197:Trees / Q000382:microbiology; D024482:Vitamin K 2 / Q000032:analysis; D053833:Wetlands
D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2