Basophil activation reveals divergent patient-specific responses to thermally processed peanuts.
BACKGROUND
The impact of processing on the allergenicity of peanut (Arachis hypogaea) proteins has traditionally been studied using immunoglobulin (Ig) E binding assay. However, as this technique does not assess the potential of an allergen to trigger basophils and mast cells, studies based on it can hardly be considered complete. We evaluated the effect of processing on peanut allergenicity using flow-cytometric quantification of in vitro basophil activation (basophil activation test [BAT]).


METHODS
Basophils from 10 patients with severe peanut allergy and 3 peanut-tolerant individuals were stimulated with extracts from 5 raw and thermally processed peanut varieties. Data were compared using protein staining (sodium dodecyl sulfate-polyacrylamide gel electrophoresis [SDS-PAGE]) and IgE immunoblotting.


RESULTS
Stimulation with different extracts resulted in patient-dependent and variety-dependent effects on basophil activation. SDS-PAGE revealed a considerable loss of identifiable bands, especially for the South Africa Common Natal, Argentina Runner, and US Virginia varieties. The results of IgE immunoblotting in patients were similar, irrespective of the responses observed in the BAT.


CONCLUSIONS
The impact of thermal processing on the capacity of peanuts to trigger basophils seems highly divergent between patients and cannot be predicted using SDS-PAGE or IgE binding. BAT can be considered a complementary tool for the evaluation of food allergenicity.
Faculty of Medicine, Department of Immunology, Allergology, Rheumatology, University of Antwerp, Antwerpen, Belgium.
V
V
Sabato
A J
AJ
van Hengel
K J
KJ
De Knop
M M
MM
Verweij
M M
MM
Hagendorens
C H
CH
Bridts
L S
LS
De Clerck
D
D
Schiavino
W J
WJ
Stevens
D G
DG
Ebo
D000328:Adult; D001491:Basophils / Q000502:physiology*; D002648:Child; D002675:Child, Preschool; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005511:Food Handling; D006358:Hot Temperature; D006801:Humans; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000276:immunology; D008297:Male; D021183:Peanut Hypersensitivity / Q000209:etiology*
D007073:Immunoglobulin E