Species identification of clinical Prevotella isolates by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
The performance of matrix-assisted laser desorption-ionization time of flight mass spectrometry (MALDI-TOF MS) for species identification of Prevotella was evaluated and compared with 16S rRNA gene sequencing. Using a Bruker database, 62.7% of the 102 clinical isolates were identified to the species level and 73.5% to the genus level. Extension of the commercial database improved these figures to, respectively, 83.3% and 89.2%. MALDI-TOF MS identification of Prevotella is reliable but needs a more extensive database.
Department of Microbiology and Infection Control, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel (VUB), Brussels, Belgium. Ingrid.wybo@uzbrussel.be
Ingrid
I
Wybo
Oriane
O
Soetens
Annelies
A
De Bel
Fedoua
F
Echahidi
Ellen
E
Vancutsem
Kristof
K
Vandoorslaer
Denis
D
Piérard
D008969:Molecular Sequence Data; D018720:Prevotella / Q000145:classification* / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012015:Reference Standards; D017423:Sequence Analysis, RNA; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000592:standards
D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S