Nanoscopy in a living mouse brain.
We demonstrated superresolution optical microscopy in a living higher animal. Stimulated emission depletion (STED) fluorescence nanoscopy reveals neurons in the cerebral cortex of a mouse with <70-nanometer resolution. Dendritic spines and their subtle changes can be observed at their relevant scales over extended periods of time.
Department of NanoBiophotonics, Max Planck Institute (MPI) for Biophysical Chemistry, Göttingen, Germany.
Sebastian
S
Berning
Katrin I
KI
Willig
Heinz
H
Steffens
Payam
P
Dibaj
Stefan W
SW
Hell
D000818:Animals; D049229:Dendritic Spines / Q000502:physiology* / Q000648:ultrastructure*; D005453:Fluorescence; D008164:Luminescent Proteins; D051379:Mice; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D036103:Nanotechnology; D013003:Somatosensory Cortex / Q000648:ultrastructure*
D008164:Luminescent Proteins