Cartilage regeneration by culturing chondrocytes in scaffolds grafted with TATVHL peptide.
Formation of neocartilage is a critical issue in contemporary regenerative medicine. This study presents the generation of tissue engineering cartilage in TATVHL peptide-grafted scaffolds. Bovine knee chondrocytes were seeded in TATVHL peptide-grafted scaffolds and cultured in a spinner bioreactor. The results revealed that surface TATVHL peptide enhanced the adhesion of bovine knee chondrocytes in scaffolds. However, an increase in the concentration of TATVHL peptide in scaffolds (up to 20 μg/mL) did not cause an evident variation in the cell viability. Surface TATVHL peptide was effective in promoting the quantity of cartilaginous components in constructs after dynamic cultivation. Biochemical assay, scanning electron microscope images, and histological staining demonstrated that surface TATVHL peptide accelerated the proliferation of bovine knee chondrocytes in constructs. In addition, the secretion of glycosaminoglycans and production of collagen in TATVHL peptide-grafted constructs were faster than those in TATVHL peptide-free constructs. TATVHL peptide can be a promising bioactive molecule to improve chondrogenesis in porous biomaterials.
Department of Chemical Engineering National Chung Cheng University Chia-Yi, Taiwan, Republic of China. chmyck@ccu.edu.tw
Yung-Chih
YC
Kuo
Cheng-Chin
CC
Wang
D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology / Q000502:physiology; D002417:Cattle; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D002470:Cell Survival; D019902:Chondrocytes / Q000166:cytology* / Q000502:physiology; D020219:Chondrogenesis / Q000502:physiology; D003094:Collagen / Q000096:biosynthesis; D006025:Glycosaminoglycans / Q000096:biosynthesis / Q000378:metabolism; D006801:Humans; D008855:Microscopy, Electron, Scanning; D009842:Oligopeptides / Q000737:chemistry*; D016062:Porosity; D012038:Regeneration; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
D006025:Glycosaminoglycans; D009842:Oligopeptides; D003094:Collagen