Anti-leishmanial activity of alkaloidal extracts obtained from different organs of Aspidosperma ramiflorum.
The present study was designated to evaluate semi-quantitative antileishmanial activity of alkaloidal extracts that were obtained from 1g of different parts of Aspidosperma ramiflorum (leaves, roots, seeds, and stem barks). Alkaloidal extracts of barks and leaves presented a good activity against the extracellular form (promastigotes) of Leishmania (L.) amazonensis. It is known that compounds responsible for the antileishmanial activity in the alkaloidal extracts from A. ramiflorum are the monoterpenoid indole alkaloids ramiflorine A and ramiflorine B, therefore extracts obtained from different plant parts were analyzed by electrospray ionization mass spectrometry (ESI-MS) in order to evidence the presence of these bioactive alkaloids. Based on these findings, alkaloidal extract from leaves was fractionated on preparative thin-layer chromatography in a bioassay-guided fractionation affording individual purified ramiflorines A and B. Both ramiflorines A and B showed significant activity against Leishmania (L.) amazonensis (LD(50) values of 18.5±6.5μg/ml and 12.63±5.52μg/ml, respectively). Our results are showing that alkaloidal extract from leaves is a promising alternative to the use of stem barks from A. ramiflorum.
Departamento de Farmácia, Universidade Estadual de Maringá, Avenida Colombo, 5790, CEP 87.020-900, Maringá, PR, Brazil.
Ananda de Castro
Ade C
Cunha
Talita Perez Cantuaria
TP
Chierrito
Gerzia Maria de Carvalho
GM
Machado
Leonor Laura Pinto
LL
Leon
Cleuza Conceição
CC
da Silva
Julio Cesar
JC
Tanaka
Lauro Mera
LM
de Souza
Regina Aparecida Correia
RA
Gonçalves
Arildo José Braz
AJ
de Oliveira
D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D030009:Aspidosperma / Q000737:chemistry*; D001681:Biological Assay; D002855:Chromatography, Thin Layer; D007891:Leishmania / Q000187:drug effects*; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D046948:Secologanin Tryptamine Alkaloids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D012639:Seeds / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization
D000470:Alkaloids; D010936:Plant Extracts; D046948:Secologanin Tryptamine Alkaloids; C000589972:ramiflorine A; C000589973:ramiflorine B