Rapid detection of aneuploidy following the generation of mutants in Candida albicans.
Techniques used to generate mutants in Candida albicans commonly result in additional and undesired genetic rearrangements. Detection of aneuploidy is, therefore, an important step forward in the quality control of mutant phenotypes. In this chapter, we describe how to extract genomic DNA and perform a quantitative multiplex PCR to compare the karyotype of any mutant strain to that of its parent and allow the detection of any unwanted aneuploidy.
School of Medical Sciences, University of Aberdeen, Aberdeen, UK. m.lenardon@abdn.ac.uk
Megan D
MD
Lenardon
André
A
Nantel
D000782:Aneuploidy; D002176:Candida albicans / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D016681:Genome, Fungal / Q000235:genetics; D007621:Karyotyping / Q000379:methods*; D060885:Multiplex Polymerase Chain Reaction; D016296:Mutagenesis / Q000235:genetics*
D004271:DNA, Fungal