Multiplexed electrochemical detection of Yersinia pestis and staphylococcal enterotoxin B using an antibody microarray.
The CombiMatrix antibody microarray is a versatile, sensitive detection platform based on the generation and transduction of electrochemical signals following antigen binding to surface antibodies. The sensor chip described herein is comprised of microelectrodes coupled to an adjacent bio-friendly matrix coated with antibodies to the biological pathogens Yersinia pestis and Bacillus anthracis, and the bacterial toxin staphylococcal enterotoxin B (SEB). Using this system, we were able to detect SEB and inactivated Y. pestis individually as well as in two-plex assays at concentrations as low as 5 pg/mL and 10(6) CFU/mL, respectively. We also introduce super avidin-biotin system (SABS) as a viable and effective means to enhance assay signal responses and lower detection limits. Together these technologies represent substantial advances in point-of-care and point-of-use detection applications.
Center for Bio, Molecular Science and Engineering, Bld. 30, US Naval Research Laboratory, 4555 Overlook Ave. SW, Washington, DC 20376, USA. jason.wojciechowski.ctr@nrl.navy.mil
Jason
J
Wojciechowski
David
D
Danley
John
J
Cooper
Nina
N
Yazvenko
Chris Rowe
CR
Taitt
D000906:Antibodies / Q000737:chemistry*; D001408:Bacillus anthracis / Q000302:isolation & purification; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods; D004768:Enterotoxins / Q000302:isolation & purification*; D006801:Humans; D008839:Microelectrodes; D019095:Point-of-Care Systems; D040081:Protein Array Analysis / Q000379:methods*; D015010:Yersinia pestis / Q000302:isolation & purification*
D000906:Antibodies; D004768:Enterotoxins; C031818:enterotoxin B, staphylococcal