Effect of magnet implant on iron biodistribution of Fe@C nanoparticles in the mouse.
The in vivo biodistribution of Fe@C nanoparticles (NP) was tested in mice bearing an inflammatory focus induced by injecting carrageenan into an air pouch previously formed on their back. The animals were intravenously injected NP with a high (60 mg/kg) or a low iron dose (6 mg/kg) and sacrificed 2 h later. Blood and organ samples (liver, spleen, lung, and kidney) were obtained; washed exudates were also collected. Iron concentration in plasma, blood cells, organs, and exudates was determined by flameless atomic-absorption-spectroscopy after digestion of organic material. Pouch exudate volume increased in all groups of mice with experimental inflammation. After i.v. administration of the high and low dose of NP, iron in exudate increased by 83.3% and 92.2%, respectively. A similar increase in hepatic iron appeared after the high dose (78%), but no increase appeared after the low dose. When the magnet was present, a 157% and 119% increase of iron in exudate appeared after both doses of NPs, but only the high dose of NP increased iron liver (60%). The presence of a magnetic field in the pouch favored selective biodistribution of NP in the inflammatory focus. These results indicate that mice with an inflammatory compartment are suitable for primary screening of different NP types. They also show that selective biodistribution is greater when a low dose of NP was used and that distribution in the target organ was increased by the magnetic field.
Departament de Farmàcia i Tecnologia Farmacèutica, Unitat de Biofarmàcia i Farmacocinètica, E-08028 Barcelona, Spain. eescribano@ub.edu
Elvira
E
Escribano
Rodrigo
R
Fernández-Pacheco
J Gabriel
JG
Valdivia
M Ricardo
MR
Ibarra
Clara
C
Marquina
Josep
J
Queralt
D000818:Animals; D005260:Female; D007501:Iron / Q000737:chemistry / Q000378:metabolism*; D060526:Magnetic Fields; D059346:Magnets; D053768:Metal Nanoparticles / Q000737:chemistry; D051379:Mice; D011897:Random Allocation; D014018:Tissue Distribution
D007501:Iron