Explants of intact endometrium to model bovine innate immunity and inflammation ex vivo.
OBJECTIVE
Bacterial infections commonly cause bovine endometritis and infertility via innate immune pathways. However, mechanistic studies using isolated cells or chopped tissue may be compromised by the disruption of endometrial architecture and release of damage-associated molecular patterns. So, this study aimed to establish an ex vivo model of intact bovine endometrium to study innate immunity and inflammation.


METHODS
Intact bovine endometrium explants were collected using a sterile 8-mm punch biopsy and cultured ex vivo with bacteria or pathogen-associated molecules. Interleukin accumulation was measured, and tissue viability was assessed by microscopy, TdT-mediated biotin-dUTP nick-end labelling and lactate dehydrogenase assay.


RESULTS
Intact endometrium explants accumulated IL-6, IL-1β and IL-8 in response to Gram-negative or Gram-positive bacteria, and their purified pathogen-associated molecules; inflammatory responses were dependent on the stage of oestrous cycle. Explants of intact endometrium maintained viability and tissue architecture, and had lower basal accumulation of interleukins compared with explants using chopped endometrium.


CONCLUSIONS
This study established a tractable ex vivo model of intact endometrium to explore the mechanisms of immunity and inflammation in the bovine endometrium.
College of Medicine, Institute of Life Science, Swansea University, UK.
Alan Maia
AM
Borges
Gareth David
GD
Healey
Iain Martin
IM
Sheldon
D000818:Animals; D055667:Arcanobacterium; D001424:Bacterial Infections / Q000276:immunology* / Q000382:microbiology / Q000662:veterinary; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology* / Q000382:microbiology; D003352:Corynebacterium; D016207:Cytokines / Q000276:immunology*; D004717:Endometrium / Q000276:immunology*; D004926:Escherichia coli; D005260:Female; D007113:Immunity, Innate; D007249:Inflammation / Q000276:immunology*; D014591:Uterine Diseases / Q000276:immunology* / Q000382:microbiology / Q000662:veterinary
D016207:Cytokines