Label-free toxin detection by means of time-resolved electrochemical impedance spectroscopy.
The real-time detection of trace concentrations of biological toxins requires significant improvement of the detection methods from those reported in the literature. To develop a highly sensitive and selective detection device it is necessary to determine the optimal measuring conditions for the electrochemical sensor in three domains: time, frequency and polarization potential. In this work we utilized a time-resolved electrochemical impedance spectroscopy for the detection of trace concentrations of Staphylococcus enterotoxin B (SEB). An anti-SEB antibody has been attached to the nano-porous aluminum surface using 3-aminopropyltriethoxysilane/glutaraldehyde coupling system. This immobilization method allows fabrication of a highly reproducible and stable sensing device. Using developed immobilization procedure and optimized detection regime, it is possible to determine the presence of SEB at the levels as low as 10 pg/mL in 15 minutes.
School of Environmental and Biological Sciences, Rutgers, State University of New Jersey, New Brunswick, NJ 08901, USA. chchai@rutgers.edu
Changhoon
C
Chai
Paul
P
Takhistov
D015374:Biosensing Techniques / Q000295:instrumentation*; D058266:Dielectric Spectroscopy / Q000295:instrumentation*; D004768:Enterotoxins / Q000032:analysis*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation*; D013194:Staining and Labeling
D004768:Enterotoxins; C026066:enterotoxin A, Staphylococcal