Cytokine measurement using cytometric bead arrays.
Cytokines can be measured by enzyme-linked immunosorbent assay (ELISA) or multiplex assay. Both techniques are commonly used in immunology to detect the presence of antibody or antigen in a sample. However, multiplex bead array technology provides the means to simultaneously measure multiple analytes in a single reaction, thereby saving time and resources. This method can detect up to 30 proteins at once, using a relatively small sample volume, without losing sensitivity, accuracy, or reproducibility. In this chapter, we describe the cytometric bead array (CBA) approach to simultaneously measure multiple cytokines in biological samples such as spleen, kidney, or serum from mice infected with the human fungal pathogen Candida albicans.
Departamento de Biología, Facultad de Ciencias, Universidad de La Serena, La Serena, Elqui, Chile.
Luis
L
Castillo
Donna M
DM
MacCallum
D000818:Animals; D002176:Candida albicans / Q000276:immunology / Q000302:isolation & purification / Q000472:pathogenicity; D016207:Cytokines / Q000032:analysis* / Q000276:immunology; D005260:Female; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D008863:Microspheres; D051381:Rats; D011919:Rats, Inbred Strains
D016207:Cytokines