Effect of polyamidoamine dendrimer G3 and G4 on skin permeation of 8-methoxypsoralene--in vivo study.
In the present study we have assessed the ability of (PAMAM) dendrimers G3 and G4 to facilitate transdermal delivery of 8-methoxypsoralen (8-MOP) in vivo. In vitro study using Franz diffusion cell revealed an enhanced transdermal flux for 8-MOP in complex with G3 and G4 dendrimer in relation to standard 8-MOP solution. In present study in vivo skin permeation potential of 8-MOP complex with G3 and G4 PAMAM dendrimer was assessed using confocal laser scanning microscopy (CLSM), which revealed an enhanced permeation of the 8-MOP to the deeper layers of the skin and significantly higher concentration in comparison with standard 8-MOP solution. Skin tissue 8-MOP concentration, evaluated by HPLC indicates that G3 and G4 PAMAM application significantly increase 8-MOP skin deposition in comparison with standard 8-MOP solutions after 1 and 2h. G4 appeared to be a more effective 8-MOP penetration enhancer than G3 PAMAM. Our results suggest the feasibility of G3 and G4 PAMAM dendrimers for transdermal delivery of 8-MOP resulting in better skin permeation and higher concentration of 8-MOP in epidermis and dermis of the drug that could help to improve effectiveness and safety of PUVA therapy.
Department of Cosmetology, University of Information Technology and Management In Rzeszów, 2 Sucharskiego Str., 35-325 Rzeszów, Poland.
Katarzyna
K
Borowska
Department of Cosmetology, University of Information Technology and Management In Rzeszów, 2 Sucharskiego Str., 35-325 Rzeszów, Poland. Electronic address: swolowiec@wsi.rzeszow.pl.
Stanisław
S
Wołowiec
Department of Cardiology, Medical University of Lublin. 8 Jaczewskiego Str. 20-954 Lublin, Poland.
Andrzej
A
Rubaj
Department of Pharmacognosy with Medicinal Plant Unit, Medical University of Lublin,1 Chodźki Str.,20-093 Lublin, Poland.
Kazimierz
K
Głowniak
Department of Pharmacognosy with Medicinal Plant Unit, Medical University of Lublin,1 Chodźki Str.,20-093 Lublin, Poland.
Elwira
E
Sieniawska
Department of Human Anatomy, Medical University of Lublin, 4 Jaczewskiego Str., 20-090 Lublin, Poland.
Sebastian
S
Radej
D000279:Administration, Cutaneous; D000818:Animals; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid; D050091:Dendrimers / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D004337:Drug Carriers; D004339:Drug Compounding; D008297:Male; D008730:Methoxsalen / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D018613:Microscopy, Confocal; D036103:Nanotechnology; D011701:PUVA Therapy; D010539:Permeability; D017319:Photosensitizing Agents / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000187:drug effects* / Q000378:metabolism; D012869:Skin Absorption / Q000187:drug effects*; D013678:Technology, Pharmaceutical / Q000379:methods
D050091:Dendrimers; D004337:Drug Carriers; C104700:PAMAM Starburst; D017319:Photosensitizing Agents; D008730:Methoxsalen