Measurement of pH values in human tissues by two-photon microscopy.
pH values go live! A ratiometric two-photon (TP) probe (NP1, see scheme) that has a significant TP action cross-section, high photostability, negligible toxicity, and can estimate pH values in live cells and human tissues by two-photon microscopy is described. NP1 can detect the difference in pH between live cells from the gastroesophageal junction (GEJ) and the lower esophageal sphincter of patients with and without esophagitis.
Department of Chemistry, Korea University, Anam-ro, Sungbuk-gu, Seoul, Korea.
Hee Jung
HJ
Park
Chang Su
CS
Lim
Eun Sun
ES
Kim
Ji Hee
JH
Han
Tae Hee
TH
Lee
Hoon Jai
HJ
Chun
Bong Rae
BR
Cho
D004935:Esophageal Diseases / Q000473:pathology; D004943:Esophagogastric Junction; D005456:Fluorescent Dyes / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D018613:Microscopy, Confocal; D009281:Naphthalenes / Q000737:chemistry*; D010080:Oxazoles / Q000737:chemistry*; D011725:Pyridines / Q000737:chemistry*
D005456:Fluorescent Dyes; D009281:Naphthalenes; D010080:Oxazoles; D011725:Pyridines; C573073:probe (NP1)