Relationship between short activated partial thromboplastin times, thrombin generation, procoagulant factors and procoagulant phospholipid activity.
Short activated partial thromboplastin times (APTTs) are associated with thrombosis. However, what short APTTs actually represent in terms of possible mechanistic pathways is not well characterized. We have assessed thrombin generation as compared with levels of procoagulant factor (fibrinogen, V, VIII, IX, XI and XII) activities, von Willebrand factor level and activity using collagen binding, as well as procoagulant phospholipid activity, in 113 consecutive samples exhibiting a short APTT compared with an equal number of age-matched and sex-matched samples yielding a normal APTT. We found a significant difference in peak thrombin generation, velocity index and area under the curve between the two groups, and that thrombin generation markers correlated with the APTT, procoagulant phospholipid activity and several procoagulant clotting factors. We conclude that short APTTs represent a procoagulant milieu, as represented by heightened thrombin generation and several other heightened procoagulant activities, which may help explain the association with thrombosis.
Department of Endocrinology, Institute of Clinical Pathology and Medical Research, Westmead Hospital, New South Wales, Australia. ashraf.mina@swahs.health.nsw.gov.au
Ashraf
A
Mina
Emmanuel J
EJ
Favaloro
Jerry
J
Koutts
D019540:Area Under Curve; D016022:Case-Control Studies; D003094:Collagen / Q000378:metabolism; D005164:Factor IX / Q000032:analysis; D005165:Factor V / Q000032:analysis; D005169:Factor VIII / Q000032:analysis; D005172:Factor XI / Q000032:analysis; D005174:Factor XII / Q000032:analysis; D005260:Female; D005340:Fibrinogen / Q000032:analysis; D006801:Humans; D008297:Male; D010314:Partial Thromboplastin Time; D010743:Phospholipids / Q000097:blood*; D011485:Protein Binding; D013050:Spectrometry, Fluorescence; D013917:Thrombin / Q000096:biosynthesis; D013925:Thromboplastin / Q000032:analysis; D013927:Thrombosis / Q000097:blood*; D014841:von Willebrand Factor / Q000032:analysis
D010743:Phospholipids; D014841:von Willebrand Factor; D005165:Factor V; D005169:Factor VIII; D005164:Factor IX; D005174:Factor XII; D005340:Fibrinogen; D003094:Collagen; D005172:Factor XI; D013925:Thromboplastin; D013917:Thrombin