[Analysis of community composition in dental plaque of elder people with root caries].
OBJECTIVE
To analyze the community in dental plaque of elder people with root caries.


METHODS
Total DNAs were extracted from the root caries dental plaques of nine elders over 60 years of age. Polymerase chaid reaction-based denaturing gradient gel electrophoresis (PCR-DGGE) was used to analyze the microbial composition, DGGE bands were excised from the gels for sequencing and identification.


RESULTS
The dominant genus in root caries dental plaque of elder people were: Acinetobacte [0.9% (1/114)], Actinobaculum [1.8% (2/114)], Actinomyces [15.8% (18/114)], Aggregatibacter [0.9% (1/114)], Capnocytophaga [14.0% (16/114)], Corynebacterium [0.9% (1/114)], Haemophilus [0.9% (1/114)], Mobiluncus [0.9% (1/114)], Naxibacter [0.9% (1/114)], Neisseriaceae [10.5% (12/114)], Porphyromonas [0.9% (1/114)], Prevotella [12.3% (14/114)], Selenomonas [6.1% (7/114)], Staphylococcus [1.8% (2/114)], Oralis streptococcus [6.1% (7/114)], Mutans streptococcu [7.9% (9/114)], Tannerella [0.9% (1/114)], Treponema [1.8% (2/114)], Veillonella [10.5% (12/114)] and two uncultured unknown genus [1.8% (2/114)]. Uncultred genotypes accounted for 19.30% of the total. Gram-positive bacteria genotype accounted for 31.6% (36/114), and Gram-negative bacteria genotype accounted for 66.7% (76/114).


CONCLUSIONS
There were many bacteria genotypes in root caries dental plaque in the elderly, which were widely distributed. Gram-negative bacteria accounted for the majority. Genotype-specific pathogenic bacteria were not found.
Department of Endodontics and Operative Dentistry, Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine & Shanghai Research Institute of Stomatology & Shanghai Key Laboratory of Stomatology, Shanghai 200011, China.
Shan-fen
SF
Ma
Jing-ping
JP
Liang
Yun-tao
YT
Jiang
Cai-lian
CL
Zhu
D000367:Age Factors; D000368:Aged; D002206:Capnocytophaga / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis; D003773:Dental Plaque / Q000382:microbiology*; D005838:Genotype; D006090:Gram-Negative Bacteria / Q000235:genetics / Q000302:isolation & purification*; D006094:Gram-Positive Bacteria / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008875:Middle Aged; D009346:Neisseriaceae / Q000235:genetics / Q000302:isolation & purification; D018720:Prevotella / Q000235:genetics / Q000302:isolation & purification; D017213:Root Caries / Q000382:microbiology*; D020357:Selenomonas / Q000235:genetics / Q000302:isolation & purification; D013295:Streptococcus mutans / Q000235:genetics / Q000302:isolation & purification; D019533:Streptococcus oralis / Q000235:genetics / Q000302:isolation & purification; D014678:Veillonella / Q000235:genetics / Q000302:isolation & purification
D004269:DNA, Bacterial