Efficacy of thiolated eudragit microspheres as an oral vaccine delivery system to induce mucosal immunity against enterotoxigenic Escherichia coli in mice.
A vaccine delivery system based on thiolated eudragit microsphere (TEMS) was studied in vivo for its ability to elicit mucosal immunity against enterotoxigenic Escherichia coli (ETEC). Groups of mice were orally immunized with F4 or F18 fimbriae of ETEC and F4 or F18 loaded in TEMS. Mice that were orally administered with F4 or F18 loaded TEMS showed higher antigen-specific IgG antibody responses in serum and antigen-specific IgA in saliva and feces than mice that were immunized with antigens only. In addition, oral vaccination of F4 or F18 loaded TEMS resulted in higher numbers of IgG and IgA antigen-specific antibody secreting cells in the spleen, lamina propria, and Peyer's patches of immunized mice than other groups. Moreover, TEMS administration loaded with F4 or F18 induced mixed Th1 and Th2 type responses based on similarly increased levels of IgG1 and IgG2a. These results suggest that F4 or F18 loaded TEMS may be a promising candidate for an oral vaccine delivery system to elicit systemic and mucosal immunity against ETEC.
Department of Infectious Diseases, College of Veterinary Medicine, KRF Zoonotic Disease Priority Research Institute, Seoul, Republic of Korea.
Won-Jung
WJ
Lee
Seungbin
S
Cha
Minkyoung
M
Shin
Myunghwan
M
Jung
Mohammad Ariful
MA
Islam
Chong-su
CS
Cho
Han Sang
HS
Yoo
D000284:Administration, Oral; D000818:Animals; D000942:Antigens, Bacterial / Q000008:administration & dosage / Q000276:immunology; D054307:Enterotoxigenic Escherichia coli / Q000276:immunology*; D004927:Escherichia coli Infections / Q000276:immunology*; D029968:Escherichia coli Proteins / Q000008:administration & dosage / Q000276:immunology; D005260:Female; D036781:Fimbriae Proteins / Q000008:administration & dosage / Q000276:immunology; D018928:Immunity, Mucosal; D007070:Immunoglobulin A / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D051379:Mice; D008813:Mice, Inbred ICR; D008863:Microspheres; D011109:Polymethacrylic Acids / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry; D014612:Vaccines / Q000008:administration & dosage / Q000276:immunology*
D000942:Antigens, Bacterial; D029968:Escherichia coli Proteins; C113053:FedA protein, E coli; D007070:Immunoglobulin A; D007074:Immunoglobulin G; C026888:K88 antigen, E coli; D011109:Polymethacrylic Acids; D013438:Sulfhydryl Compounds; D014612:Vaccines; D036781:Fimbriae Proteins; C038300:methylmethacrylate-methacrylic acid copolymer