Simultaneous determination of lactate and pyruvate in human sweat using reversed-phase high-performance liquid chromatography: a noninvasive approach.
The simultaneous determination of lactate and pyruvate in sweat has been performed using reversed phase high-performance liquid chromatography (RP-HPLC) with UV detection at 220 nm. The calibration curves were linear in the investigated range 0.3 - 350 mm of lactate, 0.003- 1 mm of pyruvate. The sensitivity was good with a limit of detection of 0.03 mm for lactate and 0.001 mm for pyruvate. Recoveries evaluated for the entire procedure were 102 ± 0.1 and 96 ± 0.1 for lactate and pyruvate, respectively. The method was successfully applied to analysis of sweat in 8 athletes at rest (pilocarpine sweating) and during physical exercise.
National Research Council of Italy, C.N.R., Istituto per i Processi Chimico-Fisici-IPCF- UOS Pisa, Area di Ricerca, Via G. Moruzzi 1, 56124, Pisa, Italy.
Simona
S
Biagi
Silvia
S
Ghimenti
Massimo
M
Onor
Emilia
E
Bramanti
D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D015444:Exercise; D005260:Female; D006801:Humans; D019344:Lactic Acid / Q000032:analysis* / Q000378:metabolism; D016014:Linear Models; D008297:Male; D019289:Pyruvic Acid / Q000032:analysis* / Q000378:metabolism; D015203:Reproducibility of Results; D012146:Rest; D012680:Sensitivity and Specificity; D013542:Sweat / Q000737:chemistry* / Q000378:metabolism
D019344:Lactic Acid; D019289:Pyruvic Acid