Microsatellite resources for Passeridae species: a predicted microsatellite map of the house sparrow Passer domesticus.
We identified microsatellite sequences of potential utility in the house sparrow (Passer domesticus) and assigned their predicted genome locations. These sequences included newly isolated house sparrow loci, which we fully characterized. Many of the newly isolated loci were polymorphic in two other species of Passeridae: Berthelot's pipit Anthus berthelotii and zebra finch Taeniopygia guttata. In total, we identified 179 microsatellite markers that were either isolated directly from, or are of known utility in, the house sparrow. Sixty-seven of these markers were designed from unique sequences that we isolated from a house sparrow genomic library. These new markers were combined with 36 house sparrow markers isolated by other studies and 76 markers isolated from other passerine species but known to be polymorphic in the house sparrow. We utilized sequence homology to assign chromosomal locations for these loci in the assembled zebra finch genome. One hundred and thirty-four loci were assigned to 25 different autosomes and eight loci to the Z chromosome. Examination of the genotypes of known-sex house sparrows for 37 of the new loci revealed a W-linked locus and an additional Z-linked locus. Locus Pdoμ2, previously reported as autosomal, was found to be Z-linked. These loci enable the creation of powerful and cost-effective house sparrow multiplex primer sets for population and parentage studies. They can be used to create a house sparrow linkage map and will aid the identification of quantitative trait loci in passerine species.
Department of Animal and Plant Sciences, University of Sheffield, Sheffield S10 2TN, UK. d.a.dawson@sheffield.ac.uk
Deborah A
DA
Dawson
Gavin J
GJ
Horsburgh
Andrew P
AP
Krupa
Ian R K
IR
Stewart
Sigrun
S
Skjelseth
Henrik
H
Jensen
Alexander D
AD
Ball
Lewis G
LG
Spurgin
Maria-Elena
ME
Mannarelli
Shinichi
S
Nakagawa
Julia
J
Schroeder
Carl
C
Vangestel
Gavin N
GN
Hinten
Terry
T
Burke
D000818:Animals; D002965:Classification / Q000379:methods*; D017931:DNA Primers / Q000235:genetics; D005838:Genotype; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D046370:Sparrows / Q000145:classification* / Q000235:genetics*
D017931:DNA Primers