IFN-gamma and IL-12B polymorphisms in women with cervical intraepithellial neoplasia caused by human papillomavirus.
The Human Papillomavirus (HPV) is a sexually transmitted organism associated with Cervical Intraepithelial Neoplasia (CIN) and cervical cancer, the second main cause of malignancy in women worldwide. The virus itself, however, is not enough to cause lesions on the cervix. Several studies suggest that some polymorphic sites changes the cytokines levels and influence the cancer development in HPV infected patients. In this study, we evaluated the presence of functional polymorphisms at +874 (T/A) IFNG and +1188 (A/C) IL-12B genes in cervical smears samples from 76 healthy women and 162 women, HPV positive, with CIN lesion--CIN I (45), CIN II (55), CIN III (53) and cervical cancer (9)--in Brazilian population. There was no significant differences in genotype (p = 0.4192) and allele (p = 0.370; OR = 1.20) distributions between CIN patients and control groups on IFNG allelic polymorphism. Moreover, for IL-12B gene, there was a significant difference in genotype (p = 0.015) and allele distribution (p = 0.014; OR = 0.5754) between the groups. When samples were stratified according to grade of cervical lesion, the AA genotype and A allele were less frequent in the group with low-grade cervical lesions than in group with high-grade cervical lesions (p = 0.0036 and p = 0.0010; OR = 0.3819, respectively), suggesting that the C allele (mutant) may protect against the emergence of CIN lesions and its progression.
Laboratório Genoma, Departamento de Biologia, Universidade Federal Rural de Pernambuco, Rua Dom Manoel de Medeiros, s/n Dois Irmãos, Recife, PE 52171-900, Brazil.
Viviane
V
do Carmo Vasconcelos de Carvalho
Jamilly Lopes
JL
de Macêdo
Camilla Albertina Dantas
CA
de Lima
Maria
M
da Conceição Gomes de Lima
Sandra
S
de Andrade Heráclio
Melânia
M
Amorim
Maria
M
de Mascena Diniz Maia
Ana Lúcia Figueiredo
AL
Porto
Paulo Roberto Eleutério
PR
de Souza
D052685:Alphapapillomavirus; D005260:Female; D005838:Genotype; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics*; D053762:Interleukin-12 Subunit p40 / Q000235:genetics*; D030361:Papillomavirus Infections / Q000150:complications* / Q000821:virology; D020641:Polymorphism, Single Nucleotide; D002583:Uterine Cervical Neoplasms / Q000453:epidemiology / Q000235:genetics* / Q000821:virology; D002578:Uterine Cervical Dysplasia / Q000453:epidemiology / Q000235:genetics* / Q000821:virology
D053762:Interleukin-12 Subunit p40; D007371:Interferon-gamma