In vitro and in vivo evaluation of drug release from semisolid dosage forms.
This study presents the in vitro and in vivo testing of anti-inflammatory drug containing creams, hydrogels and organogels for dermal use. In vitro penetration studies were performed with products by measuring the diffused drug amount through synthetic membranes soaked in isopropyl myristate (IPM). Our developed preparations were investigated under in vitro conditions together with two marketed medicinal products used as reference preparations. In vivo studies were carried out on anaesthetized male Wistar rats; the carrageenan-induced paw oedema decreasing effect of twelve different formulations and the reference products were measured in comparison with a control group. All - previously in vitro screened - selected products reduced paw oedema in rats. Significant differences were found among the developed products both in vitro and in vivo. Correlation between the in vitro penetration studies and in vivo results were found in the case of o/w creams, organogels and hydrogels.
Institute of Drug Regulatory Affairs, University of Szeged, Szeged, Hungary. petro@pharm.u-szeged.hu
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Petró
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Balogh
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Blazsó
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Erös
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Csóka
D000465:Algorithms; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000493:pharmacokinetics; D002351:Carrageenan; D002626:Chemistry, Pharmaceutical; D004008:Diclofenac / Q000008:administration & dosage / Q000493:pharmacokinetics; D015194:Diffusion Chambers, Culture; D004304:Dosage Forms; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D005079:Excipients; D020100:Hydrogels; D066298:In Vitro Techniques; D016014:Linear Models; D008297:Male; D008567:Membranes, Artificial; D009226:Myristates / Q000737:chemistry; D051381:Rats; D012015:Reference Standards; D012869:Skin Absorption; D013056:Spectrophotometry, Ultraviolet
D000894:Anti-Inflammatory Agents, Non-Steroidal; D004304:Dosage Forms; D005079:Excipients; D020100:Hydrogels; D008567:Membranes, Artificial; D009226:Myristates; C008205:isopropyl myristate; D004008:Diclofenac; D002351:Carrageenan