Changes in mRNA expression of adenosine receptors in human chronic periodontitis.
OBJECTIVE
To elucidate the aetiology of periodontitis, this study focused on the adenosine receptor (AR) expression profiles (A1AR, A2AAR, A2BAR and A3AR) in periodontal diseased tissues.


METHODS
Adenosine receptor gene expression levels in human gingiva from 15 patients with healthy gingival tissues (control group) and 15 patients who exhibited severe chronic periodontitis (test group) were measured using quantitative reverse transcription-polymerase chain reaction (RT-PCR).


RESULTS
The mRNA expression pattern changed in human chronic periodontitis: the A1AR decreased 20%, A2AAR increased 2.5-fold, A2BAR increased 3.7-fold and A3AR decreased 70% as compared with that of healthy gingiva.


CONCLUSIONS
Inflammation of the gingival tissue is associated with (1) an unchanged expression of A1AR, (2) an increased expression of A2AAR and A2BAR, and (3) a decreased expression of A3AR. Logistic regression analysis indicated that the change in the expression patterns can be used to diagnose/predict periodontitis. This finding indicates that the adenosine receptor expression profile is changed in periodontitis with the potential for future clinical application.
Department of Periodontics, Loma Linda University, Loma Linda, CA, USA.
Chun-Xiao
CX
Sun
Nathan R
NR
Wall
Nikola
N
Angelov
Craig
C
Ririe
Jung Wei
JW
Chen
Danilo S
DS
Boskovic
Jeffrey M
JM
Henkin
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016301:Alveolar Bone Loss / Q000378:metabolism; D055113:Chronic Periodontitis / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005881:Gingiva / Q000378:metabolism; D005884:Gingival Hemorrhage / Q000378:metabolism; D005891:Gingivitis / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D017622:Periodontal Attachment Loss / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis*; D060888:Real-Time Polymerase Chain Reaction; D043682:Receptor, Adenosine A1 / Q000032:analysis / Q000235:genetics; D043705:Receptor, Adenosine A2A / Q000032:analysis / Q000235:genetics; D043704:Receptor, Adenosine A2B / Q000032:analysis / Q000235:genetics; D043684:Receptor, Adenosine A3 / Q000032:analysis / Q000235:genetics; D018047:Receptors, Purinergic P1 / Q000032:analysis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult
D012333:RNA, Messenger; D043682:Receptor, Adenosine A1; D043705:Receptor, Adenosine A2A; D043704:Receptor, Adenosine A2B; D043684:Receptor, Adenosine A3; D018047:Receptors, Purinergic P1