Construction of an infectious cDNA clone of Culex flavivirus, an insect-specific flavivirus from Culex mosquitoes.
Culex flavivirus (CxFV) is an insect-specific flavivirus that has recently been detected in various Culex spp. mosquitoes worldwide. Here, we report the successful construction of a full-length infectious cDNA clone of a Tokyo strain, CxFV-NIID21. The full-length CxFV-NIID21 cDNA was cloned into the low-copy-number plasmid pMW119, which was stably amplified in Escherichia coli. Transfection of a mosquito cell line with in vitro-transcribed RNA from the cDNA clone resulted in the production of recombinant progeny virus with growth properties, cytopathogenicity, and virion morphology similar to the parental virus.
Department of Medical Entomology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan. hisawa@nih.go.jp
Haruhiko
H
Isawa
Ryusei
R
Kuwata
Shigeru
S
Tajima
Keita
K
Hoshino
Toshinori
T
Sasaki
Tomohiko
T
Takasaki
Mutsuo
M
Kobayashi
Kyoko
K
Sawabe
D000818:Animals; D002460:Cell Line; D003465:Culex / Q000821:virology*; D018076:DNA, Complementary / Q000235:genetics* / Q000378:metabolism; D005416:Flavivirus / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics* / Q000378:metabolism; D013045:Species Specificity
D018076:DNA, Complementary; D012367:RNA, Viral