PL1 fusion gene: a novel visual selectable marker gene that confers tolerance to multiple abiotic stresses in transgenic tomato.
Visual selectable markers, including the purple color caused by the accumulation of anthocyanins, have been proposed for use as antibiotic-free alternatives. However, the excessive accumulation of anthocyanins seriously inhibits the growth and development of transgenic plants. In our study, the AtDWF4 promoter from Arabidopsis and the tomato LeANT1 gene, encoding a MYB transcription factor, were used to construct the PL1 fusion gene to test whether it could be used as a visual selectable marker gene for tomato transformation. All the PL1 transgenic shoots exhibited intense purple color on shoot induction medium. In the transgenic tomato plants, PL1 was highly expressed in the cotyledons, but expressed only slightly in the true leaves and other organs. The expression of PL1 had no significantly adverse effects on the growth or development of the transgenic tomato plants, and conferred tolerance to multiple abiotic stresses in them. With the “cut off green shoots” method, multiple independent 35S::GFP transgenic tomato lines were successfully obtained using PL1 as the selectable marker gene. These results suggest that PL1 has potential application of visual selectable marker gene for tomato transformation.
Department of Plant Biology and Ecology, College of Life Sciences, Nankai University, Tianjin, 300071, China.
Feng
F
Jin
Shu
S
Li
Lijie
L
Dang
Wenting
W
Chai
Pengli
P
Li
Ning Ning
NN
Wang
D000222:Adaptation, Physiological; D000872:Anthocyanins / Q000235:genetics / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D003116:Color; D018548:Cotyledon / Q000235:genetics / Q000502:physiology; D003470:Culture Media / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000378:metabolism; D018551:Solanum lycopersicum / Q000235:genetics / Q000502:physiology*; D018613:Microscopy, Confocal; D010641:Phenotype; D010858:Pigmentation; D018515:Plant Leaves / Q000235:genetics / Q000502:physiology; D018520:Plant Shoots / Q000502:physiology; D030821:Plants, Genetically Modified / Q000235:genetics / Q000502:physiology; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014170:Transformation, Genetic
C111451:AT3G50660 protein, Arabidopsis; D000872:Anthocyanins; D029681:Arabidopsis Proteins; D015415:Biomarkers; D003470:Culture Media; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D049452:Green Fluorescent Proteins; D003577:Cytochrome P-450 Enzyme System