Whole genome sequencing and biological characterization of Duck/JS/10, a new lentogenic class I Newcastle disease virus.
A lentogenic Newcastle disease virus (NDV), Duck/JS/10 (JS10), was isolated from an unvaccinated duck in China. The complete genome of the virus contained 15,198 nucleotides. Based on length of the genome and a partial sequence of the F gene, the virus was classified as a class I genotype 4 NDV. The antigenicity of the virus was compared with that of NDV strain La Sota via hemagglutination inhibition (HI), virus neutralization (VN) assay and animal experiments. Our results show that JS10 generates higher HI and VN titers than La Sota against both class I and II virulent NDV strains. Experiments on animals demonstrate that virus shedding from chickens vaccinated with JS10 is significantly reduced when compared to those vaccinated with La Sota. Overall, this study strongly suggests that JS10 may qualify as a new vaccine candidate against Newcastle disease.
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 518 Ziyue Road, Shanghai 200241, People's Republic of China.
Chunchun
C
Meng
Xvsheng
X
Qiu
Shiqiang
S
Jin
Shengqing
S
Yu
Hongjun
H
Chen
Chan
C
Ding
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D002645:Chickens; D002681:China; D004372:Ducks / Q000821:virology; D016679:Genome, Viral; D008969:Molecular Sequence Data; D009521:Newcastle Disease / Q000276:immunology / Q000821:virology*; D009522:Newcastle disease virus / Q000145:classification / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification*; D010802:Phylogeny; D011201:Poultry Diseases / Q000276:immunology / Q000821:virology*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology
D000914:Antibodies, Viral; D014764:Viral Proteins