Nemesia root hair response to paper pulp substrate for micropropagation.
Agar substrates for in vitro culture are well adapted to plant micropropagation, but not to plant rooting and acclimatization. Conversely, paper-pulp-based substrates appear as potentially well adapted for in vitro culture and functional root production. To reinforce this hypothesis, this study compares in vitro development of nemesia on several substrates. Strong differences between nemesia roots growing in agar or in paper-pulp substrates were evidenced through scanning electron microscopy. Roots developed in agar have shorter hairs, larger rhizodermal cells, and less organized root caps than those growing on paper pulp. In conclusion, it should be noted that in this study, in vitro microporous substrates such as paper pulp lead to the production of similar root hairs to those found in greenhouse peat substrates. Consequently, if agar could be used for micropropagation, rooting, and plant acclimatization, enhancement could be achieved if rooting stage was performed on micro-porous substrates such as paper pulp.
Groupement de Recherche Eau, Sol, Environnement (GRESE EA4330), Laboratoire de Botanique et Cryptogamie, Faculté de Pharmacie, Université de Limoges, 2 rue du Docteur Marcland, 87025 Limoges Cedex, France. pascal.labrousse@unilim.fr
Pascal
P
Labrousse
David
D
Delmail
Raphaël
R
Decou
Michel
M
Carlué
Sabine
S
Lhernould
Pierre
P
Krausz
D000064:Acclimatization; D000362:Agar / Q000494:pharmacology; D003470:Culture Media / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D010209:Paper; D059828:Plant Cells / Q000187:drug effects; D018517:Plant Roots / Q000187:drug effects* / Q000254:growth & development* / Q000378:metabolism / Q000648:ultrastructure; D025341:Principal Component Analysis; D028083:Scrophulariaceae / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D013499:Surface Properties; D046509:Tissue Culture Techniques / Q000379:methods*; D014867:Water / Q000378:metabolism
D003470:Culture Media; D014867:Water; D000362:Agar