Species-specific identification of seven vegetable oils based on suspension bead array.
Species adulteration of vegetable oils has become a main form of adulteration in vegetable oils, severely violating consumer rights and causing disorder in the market. A reliable method of species authentication of vegetable oils is desirable. This paper reports a novel method for identification of seven species of vegetable oils based on suspension bead array. One pair of universal primers and seven species-specific probes were designed targeting rbcl gene of the chloroplast. Each probe was coupled to a unique color-coded microsphere. Biotinylated PCR amplicons of seven oils were hybridized to the complementary probes on microsphere sets. Bound amplicons were detected fluorometrically using a reporter dye, streptavidin-R-phycoeryt hrin (SA-PE). A sample could be analyzed less than 1 h after PCR amplification. With the exception of olive probe, all probes showed no cross-reactivity with other species. Absolute detection limit of the seven probes ranged from 0.01 ng/μL to 0.0001 ng/μL. Detection limit in DNA mixture was from 10% to 5%. Detection of vegetable oils validated the effectiveness of the method. The suspension bead array as a rapid, sensitive, and high-throughput technology has potential to identify more species of vegetable oils with increased species of probes.
Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing, People's Republic of China.
Yuanyuan
Y
Li
Yajun
Y
Wu
Jianxun
J
Han
Bin
B
Wang
Yiqiang
Y
Ge
Ying
Y
Chen
D001483:Base Sequence; D018744:DNA, Plant / Q000032:analysis*; D005434:Flow Cytometry / Q000379:methods; D005506:Food Contamination / Q000032:analysis* / Q000517:prevention & control; D008863:Microspheres; D008969:Molecular Sequence Data; D010938:Plant Oils / Q000737:chemistry* / Q000145:classification*; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D013045:Species Specificity
D018744:DNA, Plant; D010938:Plant Oils