Roseomonas riguiloci sp. nov., isolated from wetland freshwater.
A non-motile, coccobacillus-shaped and pink pigmented bacterium, designated strain 03SU10-P(T), was isolated from wetland freshwater (Woopo wetland, Republic of Korea). Cells were Gram reaction-negative and catalase- and oxidase-positive. The major fatty acids (>10% of total) were C(18:1)ω7c and summed feature 3 (iso-C(15:0) 2-OH and/or C(16:1)ω7c). The predominant respiratory lipoquinone was Q-10. The DNA G+C content was 68 mol%. The major polar lipids were phosphatidylethanolamine, phosphatidylcholine and an unknown aminolipid. Spermidine, putrescine and 1,3-diaminopropane were the major polyamines. A phylogenetic tree based on 16S rRNA gene sequence comparisons showed that strain 03SU10-P(T) formed an evolutionary lineage within the radiation enclosing the members of the genus Roseomonas. The nearest neighbour to the novel strain was Roseomonas stagni HS-69(T) (96.3% gene sequence similarity). The evidence provided by the polyphasic taxonomic approach used in this study indicated that strain 03SU10-P(T) could not be assigned to any recognized species; therefore a novel species is proposed, Roseomonas riguiloci sp. nov., with 03SU10-P(T) ( = KCTC 23339(T) = JCM 17520(T)) as the type strain.
Department of Biology, College of Life Science and Natural Resources, Sunchon National University, Suncheon 540-742, Republic of Korea.
Keun Sik
KS
Baik
Department of Biology, College of Life Science and Natural Resources, Sunchon National University, Suncheon 540-742, Republic of Korea.
Seong Chan
SC
Park
Department of Biology, College of Life Science and Natural Resources, Sunchon National University, Suncheon 540-742, Republic of Korea.
Han Na
HN
Choe
Korea Basic Science Institute, Suncheon Center, Suncheon 540-742, Republic of Korea.
Se Na
SN
Kim
Department of Food Science and Technology and Functional Food Research Center, Chonnam National University, Gwangju 500-757, Republic of Korea.
Jae-Hak
JH
Moon
Department of Biology, College of Life Science and Natural Resources, Sunchon National University, Suncheon 540-742, Republic of Korea.
Chi Nam
CN
Seong
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005618:Fresh Water / Q000382:microbiology*; D042082:Methylobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010802:Phylogeny; D011073:Polyamines / Q000032:analysis; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D053833:Wetlands
D004269:DNA, Bacterial; D005227:Fatty Acids; D011073:Polyamines; D011809:Quinones; D012336:RNA, Ribosomal, 16S