Rapid identification of Enterococcus faecalis by species-specific primers based on the genes involved in the Entner-Doudoroff pathway.
In this study we report a novel method for identification of Enterococcus faecalis based on polymerase chain reaction with primers specific for the eda-genes encoding the enzymes involved in the Entner-Doudoroff pathway, a pathway present only in this species among Gram-positive bacteria. The designed primers were checked in several different Enterococcus species, and with some other Gram-positive and Gram-negative bacterial species as well. Five primer combinations were used to detect the eda-1 gene, and another three for the eda-2 gene. With the exception of one of the primer combinations, all the others gave as results the expected amplification products only in E. faecalis strains.
Department of Genetics, Faculty of Biology, Sofia University St. Kliment Ohridski, Sofia, Bulgaria.
Slavil Z
SZ
Peykov
Vanya D
VD
Aleksandrova
Svetoslav G
SG
Dimov
D000446:Aldehyde-Lyases / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques; D013293:Enterococcus faecalis / Q000235:genetics*; D006836:Hydro-Lyases / Q000235:genetics*; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D000446:Aldehyde-Lyases; C100131:phospho-2-keto-3-deoxy-gluconate aldolase; D006836:Hydro-Lyases; C022362:phosphogluconate dehydratase