Recognition of enteroinvasive Escherichia coli and Shigella flexneri by dendritic cells: distinct dendritic cell activation states.
The innate and adaptive immune responses of dendritic cells (DCs) to enteroinvasive Escherichia coli (EIEC) infection were compared with DC responses to Shigella flexneri infection. EIEC triggered DCs to produce interleukin (IL)-10, IL-12 and tumour necrosis factor (TNF)-α, whereas S. flexneri induced only the production of TNF-α. Unlike S. flexneri, EIEC strongly increased the expression of toll like receptor (TLR)-4 and TLR-5 in DCs and diminished the expression of co-stimulatory molecules that may cooperate to inhibit CD4(+) T-lymphocyte proliferation. The inflammation elicited by EIEC seems to be related to innate immunity both because of the aforementioned results and because only EIEC were able to stimulate DC transmigration across polarised Caco-2 cell monolayers, a mechanism likely to be associated with the secretion of CC chemokine ligands (CCL)20 and TNF-α. Understanding intestinal DC biology is critical to unravelling the infection strategies of EIEC and may aid in the design of treatments for infectious diseases.
Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, SP, Brasil. carol@usp.br
Ana Carolina Ramos
AC
Moreno
Karen Spadari
KS
Ferreira
Lucas Gonçalves
LG
Ferreira
Sandro Rogério de
SR
Almeida
Marina Baquerizo
MB
Martinez
D000818:Animals; D018791:CD4 Lymphocyte Count; D049109:Cell Proliferation; D003713:Dendritic Cells / Q000276:immunology*; D004926:Escherichia coli / Q000276:immunology*; D006801:Humans; D007113:Immunity, Innate / Q000276:immunology*; D018928:Immunity, Mucosal / Q000276:immunology*; D016753:Interleukin-10 / Q000096:biosynthesis; D018664:Interleukin-12 / Q000096:biosynthesis; D007413:Intestinal Mucosa / Q000166:cytology / Q000276:immunology / Q000382:microbiology*; D051189:Murinae; D012763:Shigella flexneri / Q000276:immunology*; D051197:Toll-Like Receptor 4 / Q000276:immunology; D051198:Toll-Like Receptor 5 / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D051197:Toll-Like Receptor 4; D051198:Toll-Like Receptor 5; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D018664:Interleukin-12