Association between TPO gene polymorphisms and Anti-TPO level in Tehranian population: TLGS.
BACKGROUND
Thyroid peroxidase (TPO) gene variations are one cause of thyroid autoimmune diseases. The aim of this study was to examine the association between the T1936C, T2229C and A2257C polymorphisms of the TPO gene and Anti-TPO level.


METHODS
In this case-control study, 188 individuals (86 males and 102 females), aged 20-80 years, were randomly selected from among the Tehran Lipid and Glucose Study (TLGS) population. A2257C and T2229C SNPs were detected with RFLP by use of BsrI and Eco57I as the restriction enzymes respectively, while the T1936C SNP was determined with ARMS-PCR.


RESULTS
In the presence of the C allele of T1936C, Anti-TPO level was significantly increased (CC: 238±43.3, CT: 47.7±15.9, TT: 74.1±11.3 IU/L p=0.002); however, this association was attenuated after adjustment for sex and age (p=0.059). No significant difference, before and after adjustment, was found in Anti-TPO level in the presence of T2229C SNP (CC: 129.1±24.5, CT: 43.5±12.6, TT: 126.5±13.8 IU/L p=0.196). The association between A2257C and Anti-TPO level was only significant after adjustment for potential confounders (p=0.007). The association between ATC and CTT haplotypes and Anti-TPO level was significant (p=0.023, 0.021 respectively), the association between CTT and Anti-TPO concentration was also significant after adjustment for sex (p=0.014).


CONCLUSIONS
The results of the present study confirmed the association between TPO gene polymorphisms and Anti-TPO level in the Tehranian population.
Obesity Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Bita
B
Faam
Maryam Sadat
MS
Daneshpour
Fereidoun
F
Azizi
Marzieh
M
Salehi
Mehdi
M
Hedayati
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001323:Autoantibodies / Q000097:blood*; D001483:Base Sequence; D016022:Case-Control Studies; D017931:DNA Primers / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D006239:Haplotypes; D050031:Hashimoto Disease / Q000201:enzymology / Q000235:genetics / Q000276:immunology; D006801:Humans; D007453:Iodide Peroxidase / Q000235:genetics* / Q000276:immunology*; D007492:Iran; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D055815:Young Adult
D001323:Autoantibodies; D017931:DNA Primers; D007453:Iodide Peroxidase