A nanoscale drug delivery carrier using nucleic acid aptamers for extended release of therapeutic.
We have synthesized, characterized, and optimized a novel nano drug-delivery carrier that utilizes the versatile properties of nucleic acid for programmable and on-demand drug release. The drug-delivery carrier consists of 15 nm gold nanoparticles (AuNPs) functionalized with drug binding DNA aptamers via single-stranded (ss) anchor DNA. The presence of anchor DNA makes the nanocarrier flexible to be reprogrammed with various aptamers. Under the optimum binding conditions (0.4 M NaCl and 4 μM DNA), a maximum of 101 ± 8 anchor DNA strands were conjugated per particle. On binding DNA-aptamer:drug complexes to AuNPs, a maximum of 35 neomycin molecules were bound per AuNP. Controlled and extended release of drug from the synthesized carrier was obtained by temperature and affinity modulations. Furthermore, for the first time, we demonstrated that neomycin could bind to DNA with very high affinity (K(d) = 98.101 nM). This DNA-based nanocarrier, designed using the principles of molecular biology, is expected to impact a number of treatment strategies.


BACKGROUND
In this basic science work, the authors demonstrate the feasibility of utilizing a novel nano drug-delivery carrier with the versatile properties of nucleic acid for programmable and on-demand drug release.
Department of Chemical Engineering, Auburn University, Auburn, Alabama, USA.
Padma
P
Sundaram
Jacek
J
Wower
Mark E
ME
Byrne
D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000378:metabolism; D001665:Binding Sites; D003692:Delayed-Action Preparations / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D009355:Neomycin / Q000008:administration & dosage*
D000900:Anti-Bacterial Agents; D052157:Aptamers, Nucleotide; D003692:Delayed-Action Preparations; D006046:Gold; D009355:Neomycin