Plasma level of calcitonin gene-related peptide in patients with polycystic ovary syndrome and its relationship to hormonal and metabolic parameters.
The aim of the study was to evaluate the plasma level of calcitonin gene-related peptide (CGRP) in patients with polycystic ovary syndrome (PCOS) and its relationship to hormonal and metabolic parameters. We also observed the effect of CGRP on testosterone (T) and estradiol (E(2)) release in cultured human granulosa cells. PCOS subjects (n=215) and matched healthy control women (n=103) at age of 22-38 years were enrolled in this study. We analyzed plasma CGRP concentrations, relationship of plasma CGRP with insulin resistance (IR), body mass index (BMI), luteinizing hormone/follicle-stimulating hormone (LH/FSH) ratio and T. The T and E(2) release levels of cultured human granulosa cells treated by CGRP were also measured. The results showed that plasma CGRP concentrations were significantly higher in women with PCOS than those of control subjects. In women with PCOS, there was a strong positive correlation between the plasma CGRP level with HOMA-IR, AUC-insulin, AUC-glucose, the ratio of LH/FSH and plasma T concentration. Human granulosa cells expressed CGRP receptor. Exogenous CGRP caused an elevation of T and E(2) released from the human granulosa cells. These findings suggest that CGRP may participate in the pathophysiological process of PCOS.
Institute of Reproduction and Stem Cell Engineering, Reproductive and Genetic Hospital of China International Trust and Investment Corporation (CITIC)-Xiangya, Central South University, Changsha 410078, China.
Zhe
Z
Zhang
Fei
F
Gong
Guang-Xiu
GX
Lu
D000328:Adult; D001786:Blood Glucose / Q000032:analysis; D015992:Body Mass Index; D015740:Calcitonin Gene-Related Peptide / Q000097:blood* / Q000494:pharmacology; D016022:Case-Control Studies; D002478:Cells, Cultured; D004958:Estradiol / Q000097:blood*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood*; D006107:Granulosa Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D007986:Luteinizing Hormone / Q000097:blood*; D011085:Polycystic Ovary Syndrome / Q000097:blood* / Q000503:physiopathology; D018015:Receptors, Calcitonin Gene-Related Peptide / Q000096:biosynthesis; D013739:Testosterone / Q000097:blood*
D001786:Blood Glucose; D007328:Insulin; D018015:Receptors, Calcitonin Gene-Related Peptide; D013739:Testosterone; D004958:Estradiol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D015740:Calcitonin Gene-Related Peptide