On-line measurement of the substrate concentrations in Pichia pastoris fermentations using FT-IR/ATR.
The glycerol and methanol concentrations in Pichia pastoris fermentations were measured on-line using Fourier transform infrared spectroscopy and an attenuated total reflection probe. Partial least squares regression was used to obtain calibration models. The models were regressed on synthetic multi-component spectra and semi-synthetic fermentation broth spectra. These were obtained by spectral addition. The accuracy for the on-line measurement of glycerol, given as standard error of prediction (SEP), was determined to 0.68 g/l, and the SEP of methanol was 0.13 g/l. We show how reliable calibration models are obtained relatively effortlessly by replacing extensive sampling from the reactor with simple mathematical manipulations of the model regression spectra.
Process Design and Systems Engineering, Department of Chemical Engineering, Abo Akademi University, Turku, Finland. John.Dahlbacka@abo.fi
John
J
Dahlbacka
Jan
J
Weegar
Niklas
N
von Weymarn
Kaj
K
Fagervik
D003470:Culture Media / Q000737:chemistry*; D005285:Fermentation; D005990:Glycerol / Q000032:analysis*; D000432:Methanol / Q000032:analysis*; D010843:Pichia / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
D003470:Culture Media; D005990:Glycerol; D000432:Methanol