DNA immobilization and detection on cellulose paper using a surface grown cationic polymer via ATRP.
Cationic polymers with various structures have been widely investigated in the areas of medical diagnostics and molecular biology because of their unique binding properties and capability to interact with biological molecules in complex biological environments. In this work, we report the grafting of a linear cationic polymer from an atom transfer radical polymerization (ATRP) initiator bound to cellulose paper surface. We show successful binding of ATRP initiator onto cellulose paper and grafting of polymer chains from the immobilized initiator with ATRP. The cellulose paper grafted polymer was used in combination with PicoGreen (PG) to demonstrate detection of nucleic acids in the nanogram range in homogeneous solution and in a biological sample (serum). The results showed specific identification of hybridized DNA after addition of PG in both solutions.
Network of Excellence for Functional Biomaterials, National University of Ireland, IDA Business Park, Dangan, Galway, Ireland.
Ahmed
A
Aied
Yu
Y
Zheng
Abhay
A
Pandit
Wenxin
W
Wang
D002412:Cations; D002482:Cellulose / Q000737:chemistry*; D004247:DNA / Q000032:analysis* / Q000097:blood; D005609:Free Radicals / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D009930:Organic Chemicals / Q000737:chemistry; D010209:Paper; D011108:Polymers / Q000737:chemistry; D013499:Surface Properties
D002412:Cations; D005609:Free Radicals; D009930:Organic Chemicals; C099476:PicoGreen; D011108:Polymers; D002482:Cellulose; D004247:DNA