Experimental viral evolution to specific host MHC genotypes reveals fitness and virulence trade-offs in alternative MHC types.
The unprecedented genetic diversity found at vertebrate MHC (major histocompatibility complex) loci influences susceptibility to most infectious and autoimmune diseases. The evolutionary explanation for how these polymorphisms are maintained has been controversial. One leading explanation, antagonistic coevolution (also known as the Red Queen), postulates a never-ending molecular arms race where pathogens evolve to evade immune recognition by common MHC alleles, which in turn provides a selective advantage to hosts carrying rare MHC alleles. This cyclical process leads to negative frequency-dependent selection and promotes MHC diversity if two conditions are met: (i) pathogen adaptation must produce trade-offs that result in pathogen fitness being higher in familiar (i.e., host MHC genotype adapted to) vs. unfamiliar host MHC genotypes; and (ii) this adaptation must produce correlated patterns of virulence (i.e., disease severity). Here we test these fundamental assumptions using an experimental evolutionary approach (serial passage). We demonstrate rapid adaptation and virulence evolution of a mouse-specific retrovirus to its mammalian host across multiple MHC genotypes. Critically, this adaptive response results in trade-offs (i.e., antagonistic pleiotropy) between host MHC genotypes; both viral fitness and virulence is substantially higher in familiar versus unfamiliar MHC genotypes. These data are unique in experimentally confirming the requisite conditions of the antagonistic coevolution model of MHC evolution and providing quantification of fitness effects for pathogen and host. These data help explain the unprecedented diversity of MHC genes, including how disease-causing alleles are maintained.
Department of Biology, University of Utah, Salt Lake City, UT 84112, USA. jason.kubinak@path.utah.edu
Jason L
JL
Kubinak
James S
JS
Ruff
Cornelius Whitney
CW
Hyzer
Patricia R
PR
Slev
Wayne K
WK
Potts
D000222:Adaptation, Physiological; D000818:Animals; D020296:Animals, Congenic; D019143:Evolution, Molecular; D005260:Female; D005622:Friend murine leukemia virus / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity / Q000502:physiology; D056084:Genetic Fitness / Q000235:genetics*; D014644:Genetic Variation; D054884:Host-Pathogen Interactions / Q000276:immunology*; D008285:Major Histocompatibility Complex / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C / Q000235:genetics / Q000276:immunology*; D011533:Proviruses / Q000235:genetics; D012192:Retroviridae Infections / Q000235:genetics / Q000276:immunology / Q000821:virology; D012641:Selection, Genetic; D013163:Splenomegaly / Q000209:etiology / Q000821:virology; D014412:Tumor Virus Infections / Q000235:genetics / Q000276:immunology / Q000821:virology; D019562:Viral Load; D014774:Virulence / Q000235:genetics*; D016662:Virus Integration; D014779:Virus Replication