Human periodontal ligament fibroblasts are the optimal cell source for induced pluripotent stem cells.
Among the various kinds of fibroblasts existing in the human body, the periodontal ligament (PDL) fibroblasts have been suggested as multipotent cells. Periodontal ligament fibroblasts are characterized by rapid turnover, a high remodeling capacity and remarkable capacity for renewal and repair. They also differentiate into osteoblasts and cementoblasts. We established iPS cells from human PDL fibroblasts by introducing the ES cell markers Oct3/4, Sox2, Nanog, Klf4 and Lin28 by retrovirus transduction, even without the oncogene c-Myc. The iPS cells established in this study expressed the ES cell markers and formed teratomas in SCID mice. The c-Myc expression level in the PDL fibroblasts was higher than that in the iPS cells by quantitative RT-PCR. Therefore, we have concluded that PDL fibroblasts could be an optimal cell source for iPS cells.
Department of Translational Research, Tsurumi University School of Dental Medicine, 2-1-3, Tsurumi, Tsurumi-ku, Yokohama, Kanagawa, 230-8501, Japan. nomura-y@tsurumi-u.ac.jp
Yoshiaki
Y
Nomura
Misao
M
Ishikawa
Yuichi
Y
Yashiro
Seetala
S
Sanggarnjanavanich
Takao
T
Yamaguchi
Chihiro
C
Arai
Koji
K
Noda
Yoshiro
Y
Takano
Yoshiki
Y
Nakamura
Nobuhiro
N
Hanada
D000293:Adolescent; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D000090062:Kruppel-Like Factor 4; D008297:Male; D051379:Mice; D016513:Mice, SCID; D010513:Periodontal Ligament / Q000378:metabolism*