Differences in responsivity of original cupric reducing antioxidant capacity and cupric-bathocuproine sulfonate assays to antioxidant compounds.
The cupric reducing antioxidant capacity (CUPRAC) method using bathocuproine sulfonate (BCS) as cuprous-chelating ligand claims to be an efficient substitute for the conventional CUPRAC assay using neocuproine; however, differences in their physical chemistry give rise to significantly varying responsivities to antioxidant compounds. The antioxidant capacity due to β-carotene (in acetone or dichloromethane) was fully measurable by conventional CUPRAC but not at all by cupric-BCS assay. Furthermore, CUPRAC exhibits distinctly stronger responses than cupric-BCS to naringenin, α-tocopherol, and bilirubin.
Department of Chemistry, Istanbul University, Avcilar, 34320 Istanbul, Turkey.
S Esin
SE
Çelik
Mustafa
M
Ozyürek
Kubilay
K
Güçlü
Reşat
R
Apak
D000975:Antioxidants / Q000737:chemistry*; D002614:Chelating Agents / Q000737:chemistry; D003300:Copper / Q000737:chemistry*; D006801:Humans; D010084:Oxidation-Reduction; D010618:Phenanthrolines / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D019207:beta Carotene / Q000737:chemistry
D000975:Antioxidants; D002614:Chelating Agents; D010618:Phenanthrolines; D019207:beta Carotene; C028559:bathocuproine sulfonate; D003300:Copper; C002701:neocuproine