Triptolide inhibits colon cancer cell proliferation and induces cleavage and translocation of 14-3-3 epsilon.
Triptolide is a diterpenoid triepoxide derived from the traditional Chinese medical herb Tripterygium wilfordii. In the present study, we demonstrated that this phytochemical attenuated colon cancer growth in vitro and in vivo. Using a proteomic approach, we found that 14-3-3 epsilon, a cell cycle- and apoptosis-related protein, was altered in colon cancer cells treated with triptolide. In this regard, triptolide induced cleavage and perinuclear translocation of 14-3-3 epsilon. Taken together, our findings suggest that triptolide may merit investigation as a potential therapeutic agent for colon cancer, and its anticancer action may be associated with alteration of 14-3-3 epsilon.
Key Laboratory of Transcriptome and Proteome of Human Diseases Supported by Guangdong Province and the Ministry of China, Southern Medical University, Guangzhou, China; School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, China.
Yawei
Y
Liu
Fangli
F
Song
William K K
WK
Wu
Minyi
M
He
Liang
L
Zhao
Xuegang
X
Sun
Hui
H
Li
Yong
Y
Jiang
Yungao
Y
Yang
Kang
K
Peng
D048948:14-3-3 Proteins / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D018906:Antineoplastic Agents, Alkylating / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003110:Colonic Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D004224:Diterpenes / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D015180:Electrophoresis, Gel, Two-Dimensional; D004852:Epoxy Compounds / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D006801:Humans; D008516:Medicine, Chinese Traditional; D051379:Mice; D008819:Mice, Nude; D008969:Molecular Sequence Data; D010616:Phenanthrenes / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014183:Transplantation, Heterologous; D029757:Tripterygium / Q000737:chemistry
D048948:14-3-3 Proteins; D018906:Antineoplastic Agents, Alkylating; D004224:Diterpenes; D004852:Epoxy Compounds; D010616:Phenanthrenes; C001899:triptolide