Coffee husk waste for fermentation production of mosquitocidal bacteria.
Coffee husk waste (CHW) discarded as bio-organic waste, from coffee industries, is rich in carbohydrates. The current study emphasizes the management of solid waste from agro-industrial residues for the production of biopesticides (Bacillus sphaericus, and B. thuringiensis subsp. israelensis), to control disease transmitting mosquito vectors. An experimental culture medium was prepared by extracting the filtrates from coffee husk. A conventional culture medium (NYSM) also was prepared. The studies revealed that the quantity of mosquitocidal toxins produced from CHW is at par with NYSM. The bacteria produced in these media, were bioassayed against mosquito vectors (Culex quinquefasciatus, Anopheles stephensi, and Aedes aegypti) and it was found that the toxic effect was statistically comparable. Cost-effective analysis have revealed that, production of biopesticides from CHW is highly economical. Therefore, the utilization of CHW provides dual benefits of effective utilization of environmental waste and efficient production of mosquitocidal toxins.
Unit of Microbiology and Immunology, Vector Control Research Centre (Indian Council of Medical Research), Ministry of Health & Family Welfare, Government of India, Indira Nagar, Pondicherry, 60 5006, India. subbiahpoopathi@rediffmail.com
Subbiah
S
Poopathi
S
S
Abidha
D000330:Aedes / Q000187:drug effects / Q000254:growth & development; D000818:Animals; D000852:Anopheles / Q000187:drug effects / Q000254:growth & development; D001407:Bacillus / Q000254:growth & development; D001413:Bacillus thuringiensis / Q000254:growth & development*; D001427:Bacterial Toxins / Q000633:toxicity*; D061046:Biological Control Agents; D003069:Coffee / Q000737:chemistry*; D003465:Culex / Q000187:drug effects / Q000254:growth & development; D009033:Culicidae / Q000187:drug effects* / Q000254:growth & development; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism; D005285:Fermentation; D007814:Larva / Q000187:drug effects / Q000254:growth & development; D007928:Lethal Dose 50; D009032:Mosquito Control; D018505:Waste Management / Q000191:economics / Q000379:methods*
D001427:Bacterial Toxins; D061046:Biological Control Agents; D003069:Coffee; D003470:Culture Media