Immunoglobulin G(1) immune complex upregulates interferon-γ-induced nitric oxide production via ERK1/2 activation in murine microglia.
Intrathecal Immunoglobulin G (IgG) is elevated in some central nervous system (CNS) diseases and microglia upregulate Fcγ receptors in various neurological disorders. However, the interaction between IgG or IgG immune complexes and microglial Fcγ receptors is not fully understood. In this study, the effect of IgG(1) immune complexes on microglia was investigated. IgG(1) immune complexes increased nitric oxide production in murine microglia in the presence of interferon (IFN)-γ. These effects were dependent upon IgG(1) immune complex-induced activation of spleen tyrosine kinase with subsequent activation of extracellular signal regulated kinase1/2. Collectively, these results indicate that IgG(1) immune complexes can exert immunomodulatory effects in various central nervous system disorders.
Department of Neuroimmunology, Research Institute of Environmental Medicine, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8601, Japan.
Bijay
B
Parajuli
Yoshifumi
Y
Sonobe
Jun
J
Kawanokuchi
Yukiko
Y
Doi
Mariko
M
Noda
Hideyuki
H
Takeuchi
Tetsuya
T
Mizuno
Akio
A
Suzumura
D000818:Animals; D000936:Antigen-Antibody Complex / Q000378:metabolism*; D002478:Cells, Cultured; D007074:Immunoglobulin G / Q000378:metabolism*; D007371:Interferon-gamma / Q000378:metabolism*; D020935:MAP Kinase Signaling System; D051379:Mice; D008810:Mice, Inbred C57BL; D017628:Microglia / Q000378:metabolism*; D009569:Nitric Oxide / Q000096:biosynthesis*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D013154:Spleen / Q000201:enzymology
D000936:Antigen-Antibody Complex; D007074:Immunoglobulin G; D009569:Nitric Oxide; D007371:Interferon-gamma; D011505:Protein-Tyrosine Kinases