Reduction of Salmonella enterica serovar enteritidis colonization in 20-day-old broiler chickens by the plant-derived compounds trans-cinnamaldehyde and eugenol.
The efficacies of trans-cinnamaldehyde (TC) and eugenol (EG) for reducing Salmonella enterica serovar Enteritidis colonization in broiler chickens were investigated. In three experiments for each compound, 1-day-old chicks (n = 75/experiment) were randomly assigned to five treatment groups (n = 15/treatment group): negative control (-ve S. Enteritidis, -ve TC, or EG), compound control (-ve S. Enteritidis, +ve 0.75% [vol/wt] TC or 1% [vol/wt] EG), positive control (+ve S. Enteritidis, -ve TC, or EG), low-dose treatment (+ve S. Enteritidis, +ve 0.5% TC, or 0.75% EG), and high-dose treatment (+ve S. Enteritidis, +ve 0.75% TC, or 1% EG). On day 0, birds were tested for the presence of any inherent Salmonella (n = 5/experiment). On day 8, birds were inoculated with ∼8.0 log(10) CFU S. Enteritidis, and cecal colonization by S. Enteritidis was ascertained (n = 10 chicks/experiment) after 24 h (day 9). Six birds from each treatment group were euthanized on days 7 and 10 after inoculation, and cecal S. Enteritidis numbers were determined. TC at 0.5 or 0.75% and EG at 0.75 or 1% consistently reduced (P < 0.05) S. Enteritidis in the cecum (≥3 log(10) CFU/g) after 10 days of infection in all experiments. Feed intake and body weight were not different for TC treatments (P > 0.05); however, EG supplementation led to significantly lower (P < 0.05) body weights. Follow-up in vitro experiments revealed that the subinhibitory concentrations (SICs, the concentrations that did not inhibit Salmonella growth) of TC and EG reduced the motility and invasive abilities of S. Enteritidis and downregulated expression of the motility genes flhC and motA and invasion genes hilA, hilD, and invF. The results suggest that supplementation with TC and EG through feed can reduce S. Enteritidis colonization in chickens.
Department of Animal Science, University of Connecticut, Storrs, Connecticut, USA.
Anup
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Kollanoor-Johny
Tyler
T
Mattson
Sangeetha Ananda
SA
Baskaran
Mary Anne
MA
Amalaradjou
Sankhiros
S
Babapoor
Benjamin
B
March
Satyender
S
Valipe
Michael
M
Darre
Thomas
T
Hoagland
David
D
Schreiber
Mazhar I
MI
Khan
Ann
A
Donoghue
Dan
D
Donoghue
Kumar
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Venkitanarayanan
D000171:Acrolein / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000302:isolation & purification; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000302:isolation & purification; D058491:Bacterial Load; D001835:Body Weight; D002432:Cecum / Q000382:microbiology*; D002645:Chickens / Q000382:microbiology*; D005054:Eugenol / Q000008:administration & dosage* / Q000302:isolation & purification; D005247:Feeding Behavior; D010936:Plant Extracts / Q000008:administration & dosage* / Q000302:isolation & purification; D012481:Salmonella Infections, Animal / Q000473:pathology / Q000517:prevention & control*; D012477:Salmonella enteritidis / Q000187:drug effects* / Q000302:isolation & purification
D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D005054:Eugenol; D000171:Acrolein; C012843:cinnamaldehyde