Mechanisms involved in biological behavior changes associated with Angptl4             expression in colon cancer cell lines.
Colorectal cancer (CRC) is one of the most common causes of cancer-related             deaths throughout the world. Angiopoietin-like-4 (Angptl4), a member of the angiopoietin             family of secreted proteins, is frequently expressed in the perinecrotic areas             of different human tumors, yet its role is still unclear in colorectal cancer.             Angptl4 mRNA expression in primary colorectal cancer tissue and seven colon cancer             cell lines was measured by semi-quantitative RT-PCR; the influence of Angptl4             expression on the colon cancer cell lines was investigated by either overexpression             or knockdown of Angptl4 in colon cancer cell lines HCT116 and HT29, respectively.             The results showed that Angptl4 mRNA is frequently expressed in human colorectal             cancer tissues and cell lines. Overexpression of Angptl4 promoted cell migration,             F-actin reorganization and formation of pseudopodia. Further investigation showed             that high Angptl4 expression was associated with an increase in ezrin/radixin/moesin             and vasodilator-stimulated phosphoprotein expression and a decrease in E-cadherin             expression. These results indicate that overexpression of Angptl4 may promote             invasion and metastasis in CRC.
Department of Colorectal Surgery, Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou, Zhejiang 310016, PR China.
Xue-Feng
XF
Huang
Jie
J
Han
Xiao-Tong
XT
Hu
Chao
C
He
D000199:Actins / Q000378:metabolism; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000075803:Angiopoietin-Like Protein 4; D042682:Angiopoietins / Q000235:genetics*; D000818:Animals; D015820:Cadherins / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D003110:Colonic Neoplasms / Q000235:genetics* / Q000473:pathology; D003598:Cytoskeletal Proteins / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D020868:Gene Silencing; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008840:Microfilament Proteins / Q000378:metabolism; D008875:Middle Aged; D010750:Phosphoproteins / Q000378:metabolism; D011554:Pseudopodia; D014183:Transplantation, Heterologous; D000098662:Vasodilator-Stimulated Phosphoprotein
C474772:ANGPTL4 protein, human; D000199:Actins; D000075803:Angiopoietin-Like Protein 4; D042682:Angiopoietins; D015820:Cadherins; D015815:Cell Adhesion Molecules; D003598:Cytoskeletal Proteins; D018960:Hyaluronan Receptors; D008565:Membrane Proteins; D008840:Microfilament Proteins; D010750:Phosphoproteins; D000098662:Vasodilator-Stimulated Phosphoprotein; C071058:moesin; C071776:radixin