High-performance column liquid chromatographic method for the simultaneous determination of buclizine, tryptophan, pyridoxine, and cyanocobalamin in tablets and oral suspension.
An HPLC method was developed and validated for the simultaneous determination of buclizine, tryptophan, pyridoxine, and cyanocobalamin in pharmaceutical formulations. The chromatographic separation was carried out on an RP-C18 column using a mobile phase gradient of methanol, 0.015 M phosphate buffer (pH 3.0), and 0.03 M phosphoric acid at a flow rate of 1.0 mL/min and UV detection at 230, 280, and 360 nm, respectively, for buclizine, tryptophan, pyridoxine, and cyanocobalamin. The method validation yielded good results with respect to linearity (r>0.999), specificity, precision, accuracy, and robustness. The RSD values for intraday and interday precision were below 1.82 and 0.63%, respectively, and recoveries ranged from 98.11 to 101.95%. The method was successfully applied for the QC analysis of buclizine, tryptophan, pyridoxine, and cyanocobalamin in tablets and oral suspension.
Universidade Federal de Santa Catarina, Laboratório de Controle de Qualidade, Centro de Ciências da Saúde, Departamento de Ciências Farmacêuticas, Campus Universitário Trindade, Bloco K, sala 207, 88040-900, Florianópolis, SC, Brazil. giskuminek@gmail.com
Gislaine
G
Kuminek
Hellen K
HK
Stulzer
Monika P
MP
Tagliari
Paulo R
PR
Oliveira
Larissa S
LS
Bernardi
Gabriela S
GS
Rauber
Simone G
SG
Cardoso
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010879:Piperazines / Q000032:analysis*; D012680:Sensitivity and Specificity; D013535:Suspensions / Q000737:chemistry; D013607:Tablets / Q000737:chemistry; D014364:Tryptophan / Q000032:analysis*; D014805:Vitamin B 12 / Q000032:analysis*; D025101:Vitamin B 6 / Q000032:analysis*
D010879:Piperazines; D013535:Suspensions; D013607:Tablets; C046894:buclizine; D025101:Vitamin B 6; D014364:Tryptophan; D014805:Vitamin B 12