[Protein expression and significance of survivin and NF-κB in hepatocellular carcinoma].
OBJECTIVE
To detect the protein expressions of survivin and NF (nuclear factor)-κB and investigate their roles in the pathogenesis of hepatocellular carcinoma.


METHODS
A total of 206 cases were diagnosed as hepatocellular carcinoma, and immunohistochemical technique was used to detect the protein expressions of survivin and NF-κB in hepatocellular carcinoma samples and normal liver tissue specimens. Statistical analysis was performed to analyze the relationship of protein expressions of survivin and NF-κB and clinico-pathological parameters and prognosis.


RESULTS
The expression of survivin was predominant in cytoplasm while that of NF-κB was in nucleolus. The positive rate of survivin protein was 78.2% in hepatocellular carcinoma and that of NF-κB was 81.1%. The positive rate of survivin and NF-κB was higher in hepatocellular carcinoma than that in benign hepatic tissue (11.7% and 15.5%). Significant differences existed in the expressions of survivin and NF-κB between hepatocellular carcinoma and normal hepatic tissue (P < 0.01). The expression of survivin was related to amicula invasion, portal vein tumor thrombus, lymph node metastasis and clinical stage in hepatocellular carcinoma (P < 0.01). The expression of NF-κB was also related to portal vein tumor thrombus, lymph node metastasis and clinical stage in hepatocellular carcinoma (P < 0.05). There was an obviously positive correlation between the expressions of survivin and NF-κB in hepatocellular carcinoma (r = 0.52, P < 0.01). Survival rate of patients for 1, 3 year of positive expression of survivin and NF-κB in hepatocellular carcinoma was 70.3% and 30.4%, respectively. And it was obviously lower than that of those of negative expression (88.5% and 61.5%). There was significant difference in the expressions of survivin and NF-κB between positive and negative subjects in 1, 3 year (P < 0.05).


CONCLUSIONS
Protein expressions of survivin and NF-κB are higher in hepatocellular carcinoma. And they play mutually promoting roles in the development of hepatocellular carcinoma. Thus, an important prognostic indicator may be adopted.
Department of Pathology, Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China.
Yi
Y
Jin
Chun-Kui
CK
Shao
Lu-Ying
LY
Tang
Jian-Yong
JY
Yang
Yong-Hui
YH
Huang
Jia-Ping
JP
Li
D006528:Carcinoma, Hepatocellular; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D008113:Liver Neoplasms; D016328:NF-kappa B / Q000378:metabolism; D011379:Prognosis
D051034:Inhibitor of Apoptosis Proteins; D016328:NF-kappa B