Pannexin 1 forms an anion-selective channel.
Pannexin 1 (Panx1) is expressed in various mammalian tissues including the brain and immune cells. Here, we present evidence that Panx1 when expressed in mammalian cells, forms anion-selective channels, with a rank order of permeabilities: NO (3) (-)> I(-) > Br (-)> Cl (-) > F (-)>> aspartate (-)≈ glutamate (-)≈ gluconate(-). Single-channel Panx1-mediated currents have a unitary conductance around 68 pS. Our results show that Panx1 assembles into a membrane anion channel with a relatively low single-channel conductance.
Faculty of Life Sciences, The University of Manchester, Smith Building, Oxford Road, Manchester, UK.
Weihong
W
Ma
Vincent
V
Compan
Wenxuan
W
Zheng
Elizabeth
E
Martin
R Alan
RA
North
Alexei
A
Verkhratsky
Annmarie
A
Surprenant
D000838:Anions / Q000378:metabolism*; D001224:Aspartic Acid / Q000378:metabolism; D002460:Cell Line; D002463:Cell Membrane Permeability / Q000502:physiology*; D002712:Chlorides / Q000378:metabolism; D017630:Connexins / Q000378:metabolism*; D018698:Glutamic Acid / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D007473:Ion Channels / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D050994:Voltage-Dependent Anion Channels / Q000378:metabolism*
D000838:Anions; D002712:Chlorides; D017630:Connexins; D007473:Ion Channels; D009419:Nerve Tissue Proteins; C492764:PANX1 protein, human; D050994:Voltage-Dependent Anion Channels; D001224:Aspartic Acid; D018698:Glutamic Acid