Epigenetic status and full-term development of bovine cloned embryos treated with trichostatin A.
We examined the comprehensive epigenetic status, including histone H3 and H4 acetylation, DNA methylation and level of mRNA transcripts of bovine somatic cell nuclear transfer (SCNT) embryos treated with trichostatin A (TSA), along with their full-term developmental efficacy. Treatment with 50 nM TSA enhanced early developmental competence; increased acetylation of two histones, H3K9K14 and H4K8, at the blastocyst stage; and maintained the DNA methylation status of the satelliteI sequence in bovine SCNT embryos. The difference in IGFBP-3 transcript levels between in vivo and SCNT embryos disappeared in SCNT embryos after treatment with 50 nM TSA. Pregnancy, full-term developmental competence and body weight at birth of offspring did not differ between SCNT embryos treated with 50 nM TSA and untreated embryos. These results suggest that treatment with TSA improves preimplantation development and changes the epigenetic status but does not promote the full-term development competence in bovine SCNT embryos.
Agriculture, Iwate University, Iwate 020-8550, Japan. kensawai@iwate-u.ac.jp
Ken
K
Sawai
Takashi
T
Fujii
Hiroki
H
Hirayama
Tsutomu
T
Hashizume
Akira
A
Minamihashi
D000818:Animals; D001755:Blastocyst / Q000166:cytology; D001835:Body Weight; D002417:Cattle; D019976:Cloning, Organism / Q000379:methods*; D019175:DNA Methylation; D004305:Dose-Response Relationship, Drug; D044127:Epigenesis, Genetic; D005260:Female; D005307:Fertilization in Vitro; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology; D006657:Histones / Q000378:metabolism; D006877:Hydroxamic Acids / Q000494:pharmacology*; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000166:cytology; D011247:Pregnancy
D056572:Histone Deacetylase Inhibitors; D006657:Histones; D006877:Hydroxamic Acids; C012589:trichostatin A