Excess copper induced oxidative stress and response of antioxidants in rice.
To investigate the effects of copper (Cu), rice plant (Oryza sativa. L. var. MSE-9) was treated with different Cu concentrations (0, 10, 50 and 100 μM) for 5 days in hydroponic condition. Gradual decrease in shoot and root growth was observed with the increase of Cu concentration and duration of treatment where maximum inhibition was recorded in root growth. Cu was readily absorbed by the plant though the maximum accumulation was found in root than shoot. Hydrogen peroxide (H(2)O(2)) production and lipid peroxidation were found increased with the elevated Cu concentration indicating excess Cu induced oxidative stress. Antioxidant enzymes superoxide dismutase (SOD), guaiacol peroxidase (GPX) and ascorbate peroxidase (APX) and glutathione reductase (GR) were effectively generated at the elevated concentrations of Cu though catalase (CAT) did not show significant variation with respect to control. Ascorbate (ASH), glutathione (GSH) and proline contents were also increased in all the Cu treated plants compared with the control. SOD isoenzyme was greatly affected by higher concentration of Cu and it was consistent with the changes of the activity assayed in solution. The present study confirmed that excess Cu inhibits growth, induced oxidative stress by inducing ROS formation while the stimulated antioxidative system appears adaptive response of rice plant against Cu induced oxidative stress. Moreover proline accumulation in Cu stress plant seems to provide additional defense against the oxidative stress.
Thorny Chanu
TC
Thounaojam
Plant Biochemistry and Molecular Biology Laboratory, Department of Life Science and Bioinformatics, School of Life Sciences, Assam University, Silchar 788011, India. thounaojam.chanu@gmail.com
Piyalee
P
Panda
P
P
Panda
Purabi
P
Mazumdar
P
P
Mazumdar
Devanand
D
Kumar
Gauri Dutta
GD
Sharma
G D
GD
Sharma
Lingaraj
L
Sahoo
L
L
Sahoo
Sanjib Kumar
SK
Panda
S K
SK
Panda
D000222:Adaptation, Physiological; D000975:Antioxidants / Q000378:metabolism*; D001205:Ascorbic Acid / Q000378:metabolism; D003300:Copper / Q000009:adverse effects* / Q000378:metabolism; D004798:Enzymes / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D007527:Isoenzymes; D015227:Lipid Peroxidation / Q000187:drug effects; D012275:Oryza / Q000187:drug effects* / Q000502:physiology; D018384:Oxidative Stress / Q000502:physiology*; D010940:Plant Proteins / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development; D011392:Proline / Q000378:metabolism; D014131:Trace Elements / Q000378:metabolism / Q000494:pharmacology*
D000975:Antioxidants; D004798:Enzymes; D007527:Isoenzymes; D010940:Plant Proteins; D014131:Trace Elements; D003300:Copper; D011392:Proline; D006861:Hydrogen Peroxide; D005978:Glutathione; D001205:Ascorbic Acid