[The standardization of technique of detection of blood cells cytokine production ex vivo].
The standardized technique to detect the blood cells cytokine production ex vivo was developed. The complex of mitogens of phytohemagglutinin P, phytohemagglutinin M, lipopolysaccharide, concanavalin A is applied to provide the mitogen activation. This approach permits to activate the different types of immunocompetent cells and to induce the secretion of cytokine representative series. It is demonstrated that the application of this technique permits to receive the reproducible results of the secretion of cytokine following the standard procedure. The standard values of spontaneous and mitogene-induced production of cytokines by blood cells of healthy human are determined.
S l
Sl
Ryzhikov
Iu G
IuG
Druzhinina
T G
TG
Riabicheva
N A
NA
Varaksin
D000328:Adult; D001773:Blood Cells / Q000187:drug effects* / Q000276:immunology*; D003208:Concanavalin A / Q000494:pharmacology; D016207:Cytokines / Q000096:biosynthesis* / Q000097:blood*; D005260:Female; D006801:Humans; D007159:Immunologic Tests / Q000592:standards; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D008934:Mitogens / Q000494:pharmacology*; D010835:Phytohemagglutinins / Q000494:pharmacology
D016207:Cytokines; D008070:Lipopolysaccharides; D008934:Mitogens; D010835:Phytohemagglutinins; C047876:phytohemagglutinin-M; C028784:phytohemagglutinin-P; D003208:Concanavalin A