Lack of C4d deposition may reveal susceptibility for ascending aortic dissection.
OBJECTIVE
Complement activation as evidenced by C4d deposition indicates immunological tissue reactivity. We sought to study the vascular reactivity of the aortic wall by characterizing C4d deposits.


METHODS
Aortic wall histology and immunohistochemistry for C4d, leukocytes, T- and B-lymphocytes, plasma cells, macrophages, endothelial cells, smooth muscle cells, cell proliferation, elastase, and Van-Gieson-staining were performed to 91 consecutive patients that underwent surgery for ascending aorta, and the samples were grouped according to presence of C4d deposits.


RESULTS
Fifty-three out of 91 patients had C4d deposits mainly within the adventitia (C4d +), whereas 38 patients lacked C4d deposits (C4d-) including decreased staining of intra-aortic vessels (p < 0.005). Intimal thickness and cellularity, together with inflammation consisting of plasma cells were increased in C4d- as compared with C4d + (p < 0.05). Receiver operating characteristic curve (ROC) analysis showed that C4d was associated with stabile nondissecting ascending aorta (AUC 0.792; SE 0.053; p = 0.000; 95% CI 0.688-0.895), but not with presence of aortitis per se (AUC 0.523; SE 0.069; p = 0.752; 95 % CI 0.388-0.658).


CONCLUSIONS
Lack of C4d may indicate active remodeling of the aortic wall leading to aortic dissection (AD). Immunologic complement factors may be amenable to diagnosis of instability after aortic surgery.
Heart Center, Tampere University Hospital, Tampere, Finland.
Eetu
E
Niinimaki
Timo
T
Paavonen
Timo
T
Valo
Matti
M
Tarkka
Ari
A
Mennander
D000368:Aged; D000784:Aortic Dissection / Q000276:immunology* / Q000473:pathology / Q000601:surgery; D001011:Aorta / Q000276:immunology* / Q000473:pathology / Q000601:surgery; D001014:Aortic Aneurysm / Q000276:immunology* / Q000473:pathology / Q000601:surgery; D001025:Aortitis / Q000276:immunology* / Q000473:pathology / Q000601:surgery; D015935:Complement C4b / Q000032:analysis*; D005260:Female; D005387:Finland; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D010446:Peptide Fragments / Q000032:analysis*; D011237:Predictive Value of Tests; D012372:ROC Curve
D010446:Peptide Fragments; D015935:Complement C4b; C032261:complement C4d