Fermentation and evaluation of Klebsiella pneumoniae and K. oxytoca on the production of 2,3-butanediol.
Klebsiella is one of the genera that has shown unbeatable production performance of 2,3-butanediol (2,3-BD), when compared to other microorganisms. In this study, two Klebsiella strains, K. pneumoniae (DSM 2026) and K. oxytoca (ATCC 43863), were selected and evaluated for 2,3-BD production by batch and fed-batch fermentations using glucose as a carbon source. Those strains' morphologies, particularly their capsular structures, were analyzed by scanning electron microscopy (SEM). The maximum titers of 2,3-BD by K. pneumoniae and K. oxytoca during 10 h batch fermentation were 17.6 and 10.9 g L(-1), respectively; in fed-batch cultivation, the strains showed the maximum titers of 50.9 and 34.1 g L(-1), respectively. Although K. pneumoniae showed higher productivity, SEM showed that it secreted large amounts of capsular polysaccharide, increasing pathogenicity and hindering the separation of cells from the fermentation broth during downstream processing.
Research and Development Center, GS Caltex Corporation, Yusung-gu, Daejeon, Republic of Korea.
Jung-Hee
JH
Cho
Chelladurai
C
Rathnasingh
Hyohak
H
Song
Bong-Woo
BW
Chung
Hee Jong
HJ
Lee
Doyoung
D
Seung
D002072:Butylene Glycols / Q000378:metabolism*; D005285:Fermentation; D041121:Klebsiella oxytoca / Q000378:metabolism*; D007711:Klebsiella pneumoniae / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning
D002072:Butylene Glycols; C026978:2,3-butylene glycol