Study on the destructive effect to inherent quality of Fritillaria thunbergii Miq. (Zhebeimu) by sulfur-fumigated process using chromatographic fingerprinting analysis.
The after-harvesting sun-dried processing of Fritillariae thunbergii bulbus (Zhebeimu) was the traditional treatment for commodity. Over recent decades the natural drying process for bulbus of Fritillariae has been replaced by sulfur-fumigation for reducing the drying duration and pest control. We used ultra-performance liquid chromatography coupled with evaporative light scattering detection (UPLC-ELSD) fingerprinting analysis and major alkaloids determination to investigate the potential damaging effect of the sulfur-fumigating process. The experimental conditions were as follows: Chromatography was proceeded on Waters Acquity UPLC BEH C(18) column; the linear gradient elution was conducted with mobile phase prepared from acetonitrile-0.02% triethylamine; the drift tube temperature was set at 40°C with a nitrogen flow-rate of 30psi, and the spray parameter was set 40%. All calibration curves showed good linear regression (R>0.9991) within the tested range. The method was validated for precision, accuracy, limit of detection and quantification. The study also has shown that sulfur-fumigated samples had significant loss of the main active compounds and a more destructive fingerprint profile when compared to the sun-dried samples.
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
Baozhong
B
Duan
Linfang
L
Huang
Shilin
S
Chen
D000470:Alkaloids / Q000032:analysis; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000737:chemistry*; D031410:Fritillaria / Q000737:chemistry*; D005651:Fumigation / Q000009:adverse effects; D058427:Hygroscopic Agents / Q000009:adverse effects; D010449:Peptide Mapping / Q000379:methods*; D010936:Plant Extracts / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry; D011786:Quality Control; D016082:Solar System; D013455:Sulfur / Q000009:adverse effects; D013678:Technology, Pharmaceutical / Q000379:methods
D000470:Alkaloids; D004365:Drugs, Chinese Herbal; D058427:Hygroscopic Agents; D010936:Plant Extracts; D013455:Sulfur; C000614236:zhebeimu