Fluorescence-based sensing of 2,4,6-trinitrotoluene (TNT) using a multi-channeled poly(methyl methacrylate) (PMMA) microimmunosensor.
Fluorescence immunoassays employing monoclonal antibodies directed against the explosive 2,4,6-trinitrotoluene (TNT) were conducted in a multi-channel microimmunosensor. The multi-channel microimmunosensor was prepared in poly (methyl methacrylate) (PMMA) via hot embossing from a brass molding tool. The multi-channeled microfluidic device was sol-gel coated to generate a siloxane surface that provided a scaffold for antibody immobilization. AlexaFluor-cadaverine-trinitrobenzene (AlexaFluor-Cad-TNB) was used as the reporter molecule in a displacement immunoassay. The limit of detection was 1-10 ng/mL (ppb) with a linear dynamic range that covered three orders of magnitude. In addition, antibody crossreactivity was investigated using hexahydro-1,3,5-triazine (RDX), HMX, 2,4-dinitrotoluene (DNT), 4-nitrotoluene (4-NT) and 2-amino-4,6-DNT.
Center for Bio/Molecular Science and Engineering (Code 6920), US Naval Research Laboratory, 4555 Overlook Ave. SW, Washington, DC 20375, USA. paul.charles@nrl.navy.mil
Paul T
PT
Charles
Andre A
AA
Adams
Peter B
PB
Howell
Scott A
SA
Trammell
Jeffrey R
JR
Deschamps
Anne W
AW
Kusterbeck
D004784:Environmental Monitoring / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000379:methods*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D008904:Miniaturization; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014159:Transducers; D014303:Trinitrotoluene / Q000032:analysis*
D014303:Trinitrotoluene; D019904:Polymethyl Methacrylate