Bacterial host interaction of GFP-labelled Vibrio anguillarum HI-610 with gnotobiotic sea bass, Dicentrarchus labrax (L.), larvae.
The location and cell damage caused by Vibrio anguillarum, the causative agent of classical vibriosis, within the developing gut of the newly hatched sea bass, Dicentrarchus labrax (L.), is unknown. A gnotobiotic sea bass model was used to investigate the early interactions of V. anguillarum with sea bass larvae. In the present study, germ-free sea bass larvae were orally exposed to a V. anguillarum HI-610 pathogen labelled with the green fluorescent protein (GFP-HI-610) and sampled at regular intervals. Pathogenic colonization of gut enterocytes was observed 2 h post-exposure (p.e.) and onwards, whereas bacteria within the swim bladder were visualized 48 h p.e and onwards. Ultrastructural findings demonstrated direct bacterial contact with the host cell in the oesophageal mucosa and putative attachment to microvilli of mid- and hindgut enterocytes. The present findings form a starting point for studies assessing the impact of potential candidates (probiotics, prebiotics, antimicrobial peptides) to mitigate bacterial virulence.
Department of Morphology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Gent, Belgium Ecloserie Marine de Gravelines, Voie des Enrochements, Gravelines, France Laboratory of Microbial Ecology and Technology (LabMET), Faculty of Bioscience Engineering, Ghent University, Gent, Belgium Department of Virology, Parasitology, and Immunology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium Department of Anatomy, Embryology and Histology, Ghent University, Gent, Belgium Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
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Rekecki
Department of Morphology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Gent, Belgium Ecloserie Marine de Gravelines, Voie des Enrochements, Gravelines, France Laboratory of Microbial Ecology and Technology (LabMET), Faculty of Bioscience Engineering, Ghent University, Gent, Belgium Department of Virology, Parasitology, and Immunology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium Department of Anatomy, Embryology and Histology, Ghent University, Gent, Belgium Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
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Gunasekara
Department of Morphology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Gent, Belgium Ecloserie Marine de Gravelines, Voie des Enrochements, Gravelines, France Laboratory of Microbial Ecology and Technology (LabMET), Faculty of Bioscience Engineering, Ghent University, Gent, Belgium Department of Virology, Parasitology, and Immunology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium Department of Anatomy, Embryology and Histology, Ghent University, Gent, Belgium Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
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Dierckens
Department of Morphology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Gent, Belgium Ecloserie Marine de Gravelines, Voie des Enrochements, Gravelines, France Laboratory of Microbial Ecology and Technology (LabMET), Faculty of Bioscience Engineering, Ghent University, Gent, Belgium Department of Virology, Parasitology, and Immunology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium Department of Anatomy, Embryology and Histology, Ghent University, Gent, Belgium Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
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Laureau
Department of Morphology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Gent, Belgium Ecloserie Marine de Gravelines, Voie des Enrochements, Gravelines, France Laboratory of Microbial Ecology and Technology (LabMET), Faculty of Bioscience Engineering, Ghent University, Gent, Belgium Department of Virology, Parasitology, and Immunology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium Department of Anatomy, Embryology and Histology, Ghent University, Gent, Belgium Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
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Boon
Department of Morphology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Gent, Belgium Ecloserie Marine de Gravelines, Voie des Enrochements, Gravelines, France Laboratory of Microbial Ecology and Technology (LabMET), Faculty of Bioscience Engineering, Ghent University, Gent, Belgium Department of Virology, Parasitology, and Immunology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium Department of Anatomy, Embryology and Histology, Ghent University, Gent, Belgium Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
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Favoreel
Department of Morphology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Gent, Belgium Ecloserie Marine de Gravelines, Voie des Enrochements, Gravelines, France Laboratory of Microbial Ecology and Technology (LabMET), Faculty of Bioscience Engineering, Ghent University, Gent, Belgium Department of Virology, Parasitology, and Immunology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium Department of Anatomy, Embryology and Histology, Ghent University, Gent, Belgium Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
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Cornelissen
Department of Morphology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Gent, Belgium Ecloserie Marine de Gravelines, Voie des Enrochements, Gravelines, France Laboratory of Microbial Ecology and Technology (LabMET), Faculty of Bioscience Engineering, Ghent University, Gent, Belgium Department of Virology, Parasitology, and Immunology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium Department of Anatomy, Embryology and Histology, Ghent University, Gent, Belgium Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
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Sorgeloos
Department of Morphology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Gent, Belgium Ecloserie Marine de Gravelines, Voie des Enrochements, Gravelines, France Laboratory of Microbial Ecology and Technology (LabMET), Faculty of Bioscience Engineering, Ghent University, Gent, Belgium Department of Virology, Parasitology, and Immunology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium Department of Anatomy, Embryology and Histology, Ghent University, Gent, Belgium Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
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Ducatelle
Department of Morphology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Gent, Belgium Ecloserie Marine de Gravelines, Voie des Enrochements, Gravelines, France Laboratory of Microbial Ecology and Technology (LabMET), Faculty of Bioscience Engineering, Ghent University, Gent, Belgium Department of Virology, Parasitology, and Immunology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium Department of Anatomy, Embryology and Histology, Ghent University, Gent, Belgium Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
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Bossier
Department of Morphology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Gent, Belgium Ecloserie Marine de Gravelines, Voie des Enrochements, Gravelines, France Laboratory of Microbial Ecology and Technology (LabMET), Faculty of Bioscience Engineering, Ghent University, Gent, Belgium Department of Virology, Parasitology, and Immunology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium Department of Anatomy, Embryology and Histology, Ghent University, Gent, Belgium Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
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Van den Broeck
D000400:Air Sacs / Q000382:microbiology; D000818:Animals; D001492:Bass / Q000382:microbiology*; D020895:Enterocytes / Q000382:microbiology; D004947:Esophagus / Q000382:microbiology; D005393:Fish Diseases / Q000382:microbiology*; D005856:Germ-Free Life; D049452:Green Fluorescent Proteins; D054884:Host-Pathogen Interactions; D007814:Larva / Q000382:microbiology; D014733:Vibrio / Q000472:pathogenicity / Q000502:physiology*; D014735:Vibrio Infections / Q000382:microbiology / Q000662:veterinary*
D049452:Green Fluorescent Proteins