Calotropis procera root extracts block VEGF-induced angiogenesis: quantitative analysis.
Angiogenesis is controlled by number of growth factors, including vascular endothelial growth factor (VEGF). Plant derived anti-angiogenic molecules acting via VEGF are being investigated for curtailing angiogenesis dependent diseases. In this study, methanolic (CM), n-hexane (CH), ethylacetate (CE) and water (CW) extracts of the roots of Calotropis procera were tested for anti-angiogenic activity. In the chicken egg chorioallantoic membrane (CAM) assay, CM, CH and CE but not CW inhibited VEGF-induced neovascularization in a dose-dependent manner. Of all the tested extracts, CM at the dose of 10, 5 and 2.5 ng most effectively inhibited over 83, 71 and 64%, of neovascularization induced by 10ng of VEGF, respectively. Sponge implantation assay in mice further showed that at the dose of 100ng CM, CH and CE but not CW significantly inhibited neovascularization induced by VEGF (100 ng). Taken together, this study indicates that the root extracts of C. procera may possess anti-angiogenic activity.
Department of Pharmacology, Delhi Institute of Pharmaceutical Sciences and Research, Pushp Vihar, M.B. Road, New Delhi - 110 017. mathurajani@yahoo.com
Rajani
R
Mathur
Suresh Kumar
SK
Gupta
Sandeep Rajinder
SR
Mathur
Thirumurthy
T
Velpandian
D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000818:Animals; D031107:Calotropis / Q000737:chemistry; D002642:Chick Embryo; D049033:Chorioallantoic Membrane / Q000098:blood supply / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D042461:Vascular Endothelial Growth Factor A / Q000037:antagonists & inhibitors*
D020533:Angiogenesis Inhibitors; D010936:Plant Extracts; D042461:Vascular Endothelial Growth Factor A