Overexpression of Snail accelerates adriamycin induction of multidrug resistance in breast cancer cells.
In addition to several molecular and morphologic changes, epithelial-mesenchymal transition (EMT) cells also show variation in sensitivity to chemotherapeutics agents. The aim of this study was to investigate whether overexpression of Snail in MCF-7 cells is associated with facilitated acquisition of P-gp mediated multidrug resistance (MDR). The results demonstrated that over-expression of Snail indeed resulted in slight enhancement of adriamycin-induced MDR in MCF-7/Snail cells without detectable increase of P-gp. However, in the longer term, MCF-7 cells overexpressing Snail were prone to be resistant to adriamycin, in this case with increased expression of P-gp. These results provide evidence that a strategy involving Snail inhibition may be a useful and promising therapeutic aspect in modulating MDR.
Dept of Pathology, Weifang Medical University, Weifang, Shandong Province, China.
Wentong
W
Li
Chuanliang
C
Liu
Yong
Y
Tang
Hongli
H
Li
Fenghua
F
Zhou
Shijun
S
Lv
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism; D000328:Adult; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000235:genetics*; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000494:pharmacology*; D018432:Drug Resistance, Multiple / Q000235:genetics; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D008875:Middle Aged; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D015820:Cadherins; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors; D004317:Doxorubicin