Protective effect of oral Lactobacilli in pathogenesis of chronic periodontitis.
The study aimed at evaluation of IL-17 and TNF-α levels and at analysis of oral lactobacilli in patients with chronic periodontitis (CP) in the context of their protective effect on a course of the disease. The study was conducted on 14 patients with moderate CP (group 1) and 14 patients with severe CP (group 2). Control group (group 3) included 15 individuals with gingivitis. Levels of IL-17 and TNF-α were estimated using an ELISA. Strains of Lactobacillus were isolated in Rogosa agar, H(2)O(2)-production was determined in TMB-Plus agar. In group 1, the mean content of IL-17 was 19.66±6.1 pg/ml, and that of TNF-α was 4.95±0.91 pg/ml, in group 2 IL-17 content was 34.7±6.65 pg/ml, and that of TNF-α was 6.94±0.78pg/ml, in group 3 content of IL-17 was 0.65±0.58pg/ml, content of TNF-α was 0.17±0.14pg/ml. Analysis of lactobacilli manifestation in the control group and in the group with moderate CP in most of the persons demonstrated presence of H(2)O(2)-producing Lactobacillus, while in the group with severe CP presence of Lactobacillus was demonstrated in only 5 patients.


CONCLUSIONS
development of CP is linked to persistent excessive cytokine response of Th17 cells, the intensity of which may affect clinical course of the disease; in parallel, H(2)O(2)-producing oral lactobacilli may prevent against progression of CP, most probably reducing secretory activity of Th17 cells and restricting growth of periodontopathogens.
Department of Conservative Dentistry and Periodontology, University of Medical Sciences, Poznan, Poland.
A K
AK
Szkaradkiewicz
T M
TM
Karpinski
A
A
Zeidler
M
M
Wyganowska-Swiatkowska
Andrzej
A
Szkaradkiewicz
D000284:Administration, Oral; D000328:Adult; D055113:Chronic Periodontitis / Q000382:microbiology* / Q000473:pathology / Q000517:prevention & control*; D018450:Disease Progression; D005260:Female; D006801:Humans; D020381:Interleukin-17 / Q000378:metabolism; D007778:Lactobacillus / Q000302:isolation & purification* / Q000378:metabolism; D008297:Male; D058504:Th17 Cells / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D020381:Interleukin-17; D014409:Tumor Necrosis Factor-alpha