Analysis of gene expression and gene silencing in human macrophages.
This unit describes how to execute a gene expression study with human macrophages. It includes protocols for human macrophage preparation, RNA extraction, real-time PCR analysis, and microarray analysis. The unit also includes a protocol for gene silencing in human macrophages. Altering gene expression can be useful to study the contribution of the gene to macrophage function or even expression of other genes.
Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom.
Fernando O
FO
Martinez
D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation / Q000235:genetics*; D020868:Gene Silencing; D006801:Humans; D008264:Macrophages / Q000276:immunology / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D012313:RNA / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification; D060888:Real-Time Polymerase Chain Reaction
D012313:RNA