Characterization of hammerhead ribozyme reactions.
Hammerhead ribozymes are small catalytic RNA motifs ubiquitously present in a large variety of genomes. The reactions catalyzed by these motifs are both their self-scission and the reverse ligation reaction. Here, we describe methods for the generation of DNA templates for the subsequent in vitro transcription of hammerhead ribozymes. This is followed by a description of the preparation of suitable RNA molecules for both reaction types, and their kinetic analysis.
Heisenberg Research Group Ribogenetics, Technical University of Darmstadt, Darmstadt, Germany.
Anne
A
Kalweit
Rita
R
Przybilski
Carsten
C
Seehafer
Marcos
M
de la Peña
Christian
C
Hammann
D004247:DNA / Q000235:genetics / Q000378:metabolism; D016678:Genome / Q000235:genetics; D007700:Kinetics; D016133:Polymerase Chain Reaction; D016337:RNA, Catalytic / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
D016337:RNA, Catalytic; C427204:hammerhead ribozyme; D004247:DNA