Poly(lactide-co-glycolide)-laponite-F68 nanocomposite vesicles through a single-step double-emulsion method for the controlled release of doxorubicin.
Poly(lactide-co-glycolide) (PLGA)-laponite-F68 nanocomposite (PNC) vesicles were prepared through a technically facile, single-step water/oil/water double-emulsion method using ethyl acetate/water mixture. Vesicles of diameter 100 nm to 1.2 μm and average membrane thickness 30 nm were produced. Encapsulation with chemotherapeutic drug doxorubicin revealed unilamellar nature of the vesicle wall. PNC exhibited exfoliated morphology, enhanced thermal stability over neat PLGA, and a glass transition temperature of 54.29 °C. The zeta potential of -14.1 ± 0.231 for the vesicles revealed that the negatively charged PLGA surface is covered with neutral F68 in the vesicle wall. F68-Assisted formation of water/oil/water double emulsion of PNC in ethyl acetate/water mixture is proposed for the formation of the vesicles. Release characteristics of doxorubicin in phosphate-buffered saline (pH 7.4), cytotoxicity of bare and drug-loaded PNC vesicles with L929 cells, and uptake of doxorubicin with C6 fibroblast glioma cell line were also investigated.
Division of Biosurface Technology, Biomedical Technology (BMT) Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology (SCTIMST), Thiruvananthapuram, Kerala, India.
Bindu P
BP
Nair
Chandra P
CP
Sharma
D002460:Cell Line; D004317:Doxorubicin / Q000737:chemistry*; D016503:Drug Delivery Systems; D004655:Emulsions; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D011092:Polyethylene Glycols / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011409:Propylene Glycols / Q000737:chemistry*; D017640:Silicates / Q000737:chemistry*
D004655:Emulsions; C116176:PEO-PPO-PEO; D011409:Propylene Glycols; D017640:Silicates; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D011092:Polyethylene Glycols; D004317:Doxorubicin; C524813:laponite