Measurement for canine IgE using canine recombinant high affinity IgE receptor α chain (FcεRIα).
To detect allergen-specific IgE in dogs with allergic diseases, we developed a recombinant canine high affinity IgE receptor α chain (FcεRIα)-based IgE detection system. Using the recombinant protein of canine FcεRIα expressed by an Escherichia coli expression system, we could detect house dust mite (Dermatophagoides farinae) allergen-specific IgE in sera from dogs naturally and experimentally sensitized to this allergen with ELISA and western blotting. The IgE binding activity of recombinant canine FcεRIα on ELISA was impaired by heat treatment of these sera. The specificity of this recombinant canine FcεRIα-based IgE detection system was confirmed by inhibition assays with canine IgE. The recombinant canine FcεRIα-based IgE detection system established in this study offers an alternative tool to measure allergen-specific IgE in dogs.
Nippon Zenyaku Kogyo Co., Ltd., 1-1 Tairanoue Sasagawa, Asaka-machi, Koriyama, Fukushima 963-0196, Japan.
Toshihiro
T
Tsukui
Masahiro
M
Sakaguchi
Keigo
K
Kurata
Sadatoshi
S
Maeda
Keitaro
K
Ohmori
Kenichi
K
Masuda
Hajime
H
Tsujimoto
Shigehiro
S
Iwabuchi
D000818:Animals; D039741:Antigens, Dermatophagoides / Q000097:blood* / Q000378:metabolism; D015153:Blotting, Western / Q000662:veterinary; D004283:Dog Diseases / Q000097:blood* / Q000175:diagnosis*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D004926:Escherichia coli; D006967:Hypersensitivity / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D007073:Immunoglobulin E / Q000097:blood* / Q000378:metabolism; D017455:Receptors, IgE / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism*; D012680:Sensitivity and Specificity
D039741:Antigens, Dermatophagoides; D017455:Receptors, IgE; D011994:Recombinant Proteins; D007073:Immunoglobulin E