Potent toxic macrocyclic trichothecenes from the marine-derived fungus Myrothecium verrucaria Hmp-F73.
Activity-guided fractionation of a methanol extract from the culture broth of Myrothecium verrucaria Hmp-F73, a fungus associated with the sponge Hymeniacidon perleve, afforded six macrocyclic trichothecenes, verrucarin J (1), 8-hydroxyverrucarin J (2), verrucarin A (3), 8-acetoxyroridin H (4), isororidin E (5), and roridin E (6), along with trichoverrin B (7). All seven metabolites displayed potent toxicity to the brine shrimp (Artemia salina). In addition, compounds 2, 3, and 6 showed weak phytotoxic activities against lettuce seeds. A preliminary structure-activity relationship of the metabolites is also discussed.
Research Centre for Natural Medicinal Chemistry, College of Science, Northwest A & F University, Yangling, Shaanxi 712100, China.
Li
L
Zhao
Li
L
Liu
Nan
N
Wang
Shu-Jin
SJ
Wang
Jing-Chun
JC
Hu
Jin-Ming
JM
Gao
D000818:Animals; D001156:Artemia; D018525:Germination / Q000187:drug effects; D006999:Hypocreales / Q000472:pathogenicity*; D007928:Lethal Dose 50; D018545:Lactuca; D011161:Porifera / Q000382:microbiology*; D012639:Seeds / Q000187:drug effects; D013329:Structure-Activity Relationship; D014255:Trichothecenes / Q000633:toxicity*
D014255:Trichothecenes