Enhanced detection sensitivity of pegylated CdSe/ZnS quantum dots-based prostate cancer biomarkers by surface plasmon-coupled emission.
We demonstrate the fabrication and detection of quantum dots (QDs)-based prostate specific antigens (PSAs) cancer protein biochips by using enhanced surface plasmon-coupled emission measurements (SPCE). The PSAs are immobilized on a SiO(2)-protected thin gold substrate and pegylated QDs which conjugated with antibodies of PSA are used as fluorescent probes. Due to the excellent brightness of the QDs and the high directionality of emission, as well as the high light collection efficiency of SPCE, the limit of detection (LOD) is down to 10 fg/mL (equal to 0.3 fM) for the PSA chips by using QDs-based cancer protein. We expect that this QDs-based SPCE measurement system with the low LOD supplies a great potential for detecting various cancer biomarkers that are present in only low concentrations within the human body.
Department of Physics and Graduate School of Nanoscience and Technology (WCU), Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea.
Li-Hua
LH
Jin
Song-Mei
SM
Li
Yong-Hoon
YH
Cho
D014408:Biomarkers, Tumor / Q000032:analysis; D019187:Cadmium Compounds / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D008297:Male; D017430:Prostate-Specific Antigen / Q000032:analysis*; D011471:Prostatic Neoplasms / Q000175:diagnosis*; D040081:Protein Array Analysis; D045663:Quantum Dots; D018036:Selenium Compounds / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013440:Sulfides / Q000737:chemistry*; D020349:Surface Plasmon Resonance / Q000379:methods*; D017967:Zinc Compounds / Q000737:chemistry*
D014408:Biomarkers, Tumor; D019187:Cadmium Compounds; D018036:Selenium Compounds; D013440:Sulfides; D017967:Zinc Compounds; C058667:cadmium selenide; D017430:Prostate-Specific Antigen; C031238:zinc sulfide