The effects of morphine and morphine conditioned context on 50 kHz ultrasonic vocalisation in rats.
The 50 kHz ultrasonic vocalisations (USVs) that are emitted by rats are dependent on activity of dopaminergic neurons projecting from the ventral tegmental area to the limbic and cortical structures. According to many experimental data, emission of the 50 kHz USV reflects a positive emotional state. The appetitive calls are also emitted in response to the administration of drugs of abuse, e.g., cocaine or amphetamine (AMPH), or in a reply to a positively conditioned context. However, there is no strong evidence in the literature that morphine can also modulate 50 kHz USVs. The aim of this paper is to study the effects of morphine and morphine-conditioned context on 50 kHz USVs, using spontaneously or drug-modulated 50 kHz USVs. Our results showed that acute administration of morphine to rats after withdrawal period inhibited the emission of 50 kHz USVs. The stimulating effect of morphine-conditioned context on 50 kHz USVs appeared on the post-withdrawal challenge day immediately before drug injection, 14 days after the last episode of morphine-induced context conditioning. The context-induced 50 kHz USVs can be used as a sensitive test for drug dependency. The current study also shows that 50 kHz USVs can be useful tool for studying the mechanisms of long lasting central effects of morphine.
Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, 26/28 Krakowskie Przedmieście Street, 00-927 Warsaw, Poland. adamhamed2005@yahoo.com
Adam
A
Hamed
Ewa
E
Taracha
Janusz
J
Szyndler
Paweł
P
Krząścik
Małgorzata
M
Lehner
Piotr
P
Maciejak
Anna
A
Skórzewska
Adam
A
Płaźnik
D000818:Animals; D003213:Conditioning, Psychological / Q000187:drug effects*; D004334:Drug Administration Schedule; D008297:Male; D009020:Morphine / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014828:Vocalization, Animal / Q000187:drug effects*
D009020:Morphine