Polysaccharides serve as scaffold of biofilms formed by mucoid Pseudomonas aeruginosa.
Chronic lung infection by mucoid Pseudomonas aeruginosa is one of the major pathologic features in patients with cystic fibrosis. Mucoid P. aeruginosa is notorious for its biofilm forming capability and resistance to immune attacks. In this study, the roles of extracellular polymeric substances from biofilms formed by mucoid P. aeruginosa were investigated. Alginate is not an essential structure component for mucoid P. aeruginosa biofilms. Genetic studies revealed that Pel and Psl polysaccharides serve as essential scaffold and mediate macrocolony formation in mucoid P. aeruginosa biofilms. The Psl polysaccharide is more important than Pel polysaccharide in mucoid P. aeruginosa biofilm structure maintenance and phagocytosis resistance. The polysaccharides were further found to protect mucoid P. aeruginosa strain from host immune clearance in a mouse model of acute lung infection.
Department of Systems Biology, Technical University of Denmark, Lyngby, Denmark. ly@bio.dtu.dk
Liang
L
Yang
Wang
W
Hengzhuang
Hong
H
Wu
Søren
S
Damkiaer
Nicholas
N
Jochumsen
Zhijun
Z
Song
Michael
M
Givskov
Niels
N
Høiby
Søren
S
Molin
D000818:Animals; D018441:Biofilms / Q000254:growth & development*; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D008168:Lung / Q000382:microbiology / Q000473:pathology; D053858:Metabolic Networks and Pathways / Q000235:genetics; D051379:Mice; D018410:Pneumonia, Bacterial / Q000382:microbiology; D011135:Polysaccharides, Bacterial / Q000378:metabolism*; D011552:Pseudomonas Infections / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism* / Q000502:physiology*
D011135:Polysaccharides, Bacterial