Sphingobacterium detergens sp. nov., a surfactant-producing bacterium isolated from soil.
A novel Gram-negative-staining strain, designated 6.2S(T), was isolated from a soil sample and identified as a biosurfactant producer. Its taxonomic position was investigated using a polyphasic approach. The cells were non-motile, non-spore-forming rods. The organism grew optimally at 30-37 °C, with 0-3% (w/v) NaCl, and at pH 7.0. Based on 16S rRNA gene sequence analysis, strain 6.2S(T) was found to be a member of the genus Sphingobacterium and was most closely related to four type species of the genus, showing sequence similarities of 96.8-98.9%. Partial chaperonin 60 (cpn60) gene sequence analysis was useful in resolving the phylogenetic relationships between strain 6.2S(T) and closely related taxa, with similarities ranging from 85.5% (with Sphingobacterium thalpophilum DSM 11723(T)) to 90.3% (with Sphingobacterium canadense CR11(T) and Sphingobacterium multivorum JCM 21156(T)). The results of DNA-DNA hybridization experiments between the novel strain and its closest relatives gave a DNA-DNA relatedness value of less than 70%, and consequently confirmed that this new strain did not belong to a previously described species of the genus Sphingobacterium. The major fatty acids were summed feature 3 (iso-C(15:0) 2 OH and/or C(16:1)ω7c); iso-C(15:0); iso-C(17:0) 3-OH and C(16:0). The G+C content of the genomic DNA was 40.0 mol%. According to its phenotypic and genotypic characteristics and the phylogenetic data, strain 6.2S(T) represents a novel species of the genus Sphingobacterium, for which the name Sphingobacterium detergens sp. nov. is proposed. The type strain is 6.2S(T) ( = CECT 7938(T) = LMG 26465(T)).
Laboratory of Microbiology, Faculty of Pharmacy, University of Barcelona, 08028 Barcelona, Spain.
Ana M
AM
Marqués
Laboratory of Microbiology, Faculty of Pharmacy, University of Barcelona, 08028 Barcelona, Spain.
César
C
Burgos-Díaz
Department of Biochemistry and Molecular Biology-A, Faculty of Veterinary, University of Murcia, 30100 Murcia, Spain.
Francisco José
FJ
Aranda
Department of Biochemistry and Molecular Biology-A, Faculty of Veterinary, University of Murcia, 30100 Murcia, Spain.
José Antonio
JA
Teruel
Laboratory of Microbiology, Faculty of Pharmacy, University of Barcelona, 08028 Barcelona, Spain.
Àngels
À
Manresa
Department of Biochemistry and Molecular Biology-A, Faculty of Veterinary, University of Murcia, 30100 Murcia, Spain.
Antonio
A
Ortiz
Laboratory of Microbiology, Faculty of Pharmacy, University of Barcelona, 08028 Barcelona, Spain.
Maribel
M
Farfán
D001394:Azores; D015373:Bacterial Typing Techniques; D001482:Base Composition; D018834:Chaperonin 60 / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D045249:Sphingobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D013501:Surface-Active Agents / Q000378:metabolism*
D018834:Chaperonin 60; D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D013501:Surface-Active Agents