A rat decellularized small bowel scaffold that preserves villus-crypt architecture for intestinal regeneration.
Management of intestinal failure remains a clinical challenge and total parenteral nutrition, intestinal elongation and/or transplantation are partial solutions. In this study, using a detergent-enzymatic treatment (DET), we optimize in rats a new protocol that creates a natural intestinal scaffold, as a base for developing functional intestinal tissue. After 1 cycle of DET, histological examination and SEM and TEM analyses showed removal of cellular elements with preservation of the native architecture and connective tissue components. Maintenance of biomechanical, adhesion and angiogenic properties were also demonstrated strengthen the idea that matrices obtained using DET may represent a valid support for intestinal regeneration.
Surgery Unit, Institute of Child Health and Great Ormond Street Hospital, University College London, London WC1N 1EH, UK.
Giorgia
G
Totonelli
Panagiotis
P
Maghsoudlou
Massimo
M
Garriboli
Johannes
J
Riegler
Giuseppe
G
Orlando
Alan J
AJ
Burns
Neil J
NJ
Sebire
Virpi V
VV
Smith
Jonathan M
JM
Fishman
Marco
M
Ghionzoli
Mark
M
Turmaine
Martin A
MA
Birchall
Anthony
A
Atala
Shay
S
Soker
Mark F
MF
Lythgoe
Alexander
A
Seifalian
Agostino
A
Pierro
Simon
S
Eaton
Paolo
P
De Coppi
D000818:Animals; D001696:Biomechanical Phenomena; D002470:Cell Survival; D005109:Extracellular Matrix / Q000737:chemistry; D007421:Intestine, Small / Q000737:chemistry / Q000166:cytology* / Q000637:transplantation / Q000648:ultrastructure*; D007422:Intestines / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration; D044968:Regenerative Medicine / Q000379:methods*; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*