A double mutant between fission yeast telomerase and RecQ helicase is sensitive to thiabendazole, an anti-microtubule drug.
In the fission yeast Schizosaccharomyces pombe, deletion of trt1(+) causes gradual telomere shortening, while deletion of pot1(+) causes rapid telomere loss. The double mutant between pot1 and RecQ helicase rqh1 is synthetically lethal. We found that the trt1 rqh1 double mutant was not synthetically lethal. The chromosome end fragments in both the trt1Δ rqh1Δ and the trt1Δ rqh1-hd (helicase dead) double mutants did not enter a pulsed-field electrophoresis gel. Both the trt1Δ rqh1Δ and the trt1Δ rqh1-hd double mutants were sensitive to the anti-microtubule drug thiabendazole. Moreover, the trt1Δ rqh1-hd double mutant displayed RPA foci on the chromosome bridge at high frequency in M phase cells. These phenotypes are very similar to that of the pot1Δ rqh1-hd double mutant, in which recombination intermediates accumulate at the chromosme ends in the M phase. These results suggest that the entangled chromosome ends, most likely recombination intermediates, are present in the M phase in the trt1Δ rqh1-hd double mutant.
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, Japan.
Shinobu
S
Ukimori
Naoki
N
Kawabata
Hideki
H
Shimada
Ryota
R
Imano
Katsunori
K
Takahashi
Masashi
M
Yukawa
Eiko
E
Tsuchiya
Masaru
M
Ueno
D000935:Antifungal Agents / Q000494:pharmacology; D015825:Chromosomes, Fungal / Q000187:drug effects; D005656:Fungal Proteins / Q000235:genetics; D008870:Microtubules / Q000187:drug effects; D009154:Mutation; D053484:RecQ Helicases / Q000235:genetics*; D012568:Schizosaccharomyces / Q000235:genetics*; D019098:Telomerase / Q000235:genetics*; D059506:Telomere Shortening / Q000187:drug effects; D013827:Thiabendazole / Q000494:pharmacology*
D000935:Antifungal Agents; D005656:Fungal Proteins; D019098:Telomerase; D053484:RecQ Helicases; D013827:Thiabendazole