[Effects of osteopontin on gap junctional intercellular communication in colon cancer cell lines].
OBJECTIVE
Liver metastasis is a major cause of mortality in patients with colorectal cancer. However, the mechanisms underlying this process remain largely unknown. Osteopontin (OPN) is a secreted phosphorylated glycoprotein known to be involved in tumor migration and metastasis. Gap junction intercellular communication (GJIC) is a pathway of direct cell to cell communication. This study analyzed gap junctional intercellular communication (GJIC) changes in colon cancer SW-480 cell lines after osteopontin had been transfected in.


METHODS
Transfect sense-osteopontin eukaryotic expression plasmids into SW-480 cell lines and detect the GJIC changes in two different cell lines by flurescence redistribution after photobleaching (FRAP).


RESULTS
Compared with control, signal exchange is impaired and GJIC function is inhibited in SW-480-pcDNA3.1(+)-OPN cell lines. FRAP after 5 min in SW-480-pcDNA3.1(+)-OPN is 24.65% ± 4.08%,the control SW-480-pcDNA3.1(+) is 44.74% ± 6.23%,P < 0.001;FRAP after 10 min in SW-480-pcDNA3.1(+)-OPN is 25.98% ± 4.48%, while SW-480-pcDNA3.1 is up to 64.92% ± 5.39%,P < 0.001.


CONCLUSIONS
OPN could inhibit GJIC function of colon cells to accelerate the metastatic process in colorectal cancer.
Department of Medical Oncology, Second Affiliated Hospital, Medical School of Zhejiang University, Hangzhou 310009, China. Email: dinglingt@gmail.com.
Ling
L
Ding
Han-Guang
HG
Hu
Shu
S
Zheng
D002450:Cell Communication; D002460:Cell Line; D003110:Colonic Neoplasms / Q000378:metabolism; D017629:Gap Junctions / Q000378:metabolism; D006801:Humans; D053495:Osteopontin
D053495:Osteopontin