Design, synthesis and evaluation of a molecularly imprinted polymer for hollow fiber-solid phase microextraction of chlorogenic acid in medicinal plants.
In this study, a simple preparation approach was developed for modified bisphenol A (BPA) molecularly imprinted polymer sorbent used in the hollow fiber solid phase microextraction (MIP-HF-SPME) of chlorogenic acid (CGA). The pre-polymer solution containing the template was introduced into the polypropylene hollow fiber segment for in situ polymerization. MIP-HF-SPME conditions based on the modified MIP-sorbent were optimized. Finally, the tool was used for selective extraction of chlorogenic acid in Echinacea purpurea, a medicinal plant. Main parameters affecting synthesis of organic-inorganic hybrid MIP and microextraction procedure were investigated and optimized. The measurements were done under the optimal conditions. The limit of detection has been gained 0.08 ng/mL. The linear range and relative standard deviation (RSD %) are 0.2-1000 ng/mL and 0.38 (n=3) respectively. The average relative recoveries of spiked analyte in the four concentration levels were between 84.8 and 97.2%.
Department of Chemistry, Payame Noor University, 19395-4697 Tehran, Iran.
Mazyar Ahmadi
MA
Golsefidi
Zarrin
Z
Es'haghi
Ali
A
Sarafraz-Yazdi
D002726:Chlorogenic Acid / Q000032:analysis / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D020900:Echinacea / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D000432:Methanol; D054802:Molecular Imprinting / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D011126:Polypropylenes / Q000737:chemistry*; D015203:Reproducibility of Results; D052617:Solid Phase Microextraction / Q000295:instrumentation / Q000379:methods*; D013010:Sonication; D013696:Temperature
D010936:Plant Extracts; D011126:Polypropylenes; D002726:Chlorogenic Acid; D000432:Methanol