Atmospheric plasma enhances wettability and cell spreading on dental implant metals.
OBJECTIVE
Treatment regimens, which predictably support re-osseointegration of implants with peri-implantitis, are needed. Increased wettability may be an important factor for re-osseointegration. In this study, a cold atmospheric pressure gas-discharge plasma was applied to reduce water contact angles on titanium discs with different surface topography and to improve the spreading of osteoblastic cells.


METHODS
An argon plasma jet with different oxygen admixtures was used to treat titanium discs with different topologies, i.e. machined, SLA(®) , SLActive(®) , diamond bur-treated or Airflow(®) -treated. Water contact angles were measured before and after plasma treatment. The spreading behaviour of human osteoblastic cells was investigated.


RESULTS
Contact angle of titanium discs (baseline values: 68°-117°) were significantly reduced close to 0° irrespective of surface topography after the application of argon plasma with 1.0% oxygen admixture for 60 s or 120 s. The cell size of osteoblastic cells grown on argon-oxygen-plasma-treated titanium discs was significantly larger than on non-treated surfaces (p < 0.001) irrespective of surface topography.


CONCLUSIONS
Plasma treatment reduced contact angle and supported spreading of osteoblastic cells. The application of cold plasma may be supportive in the treatment of peri-implant lesions and may improve the process of re-osseointegration.
Unit of Periodontology, Department of Restorative Dentistry, Periodontology and Endodontology, Dental School, Ernst-Moritz-Arndt-University Greifswald, Greifswald, Germany.
Kathrin
K
Duske
Ina
I
Koban
Eckhard
E
Kindel
Karsten
K
Schröder
Barbara
B
Nebe
Birte
B
Holtfreter
Lukasz
L
Jablonowski
Klaus D
KD
Weltmann
Thomas
T
Kocher
D001128:Argon; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D048430:Cell Shape; D048429:Cell Size; D045662:Dental Etching; D006801:Humans; D016014:Linear Models; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D010100:Oxygen; D058626:Plasma Gases / Q000737:chemistry; D014025:Titanium; D017155:Wettability
D058626:Plasma Gases; D001128:Argon; D014025:Titanium; D010100:Oxygen