Murine model of concurrent oral and vaginal Candida albicans colonisation.
Investigations into the complex interaction between the fungal pathogen Candida albicans and its human host require the use of animals as in vivo models. A major advance is the creation of a low-oestrogen murine model of concurrent oral and vaginal C. albicans colonisation that resembles human candidal carriage at both mucosal sites. Weekly intramuscular (5 μg) and subcutaneous (5 μg) oestrogen administration was determined as optimal, enhancing oral colonisation but essential for vaginal colonisation. Using a clinical C. albicans oral isolate, persistent colonisation for up to 6 weeks can be achieved at both sites in two strains of mice (BALB/c and C57BL/6). This concurrent model of mucosal colonisation reduces the numbers of experimental mice by half, and opens up new avenues of research in assessing potential mucosal vaccine candidates and in studying delicate host-pathogen interactions during the most natural state of C. albicans epithelial colonisation.
Department of Oral Immunology, King's College London Dental Institute, London, UK.
Durdana
D
Rahman
Mukesh
M
Mistry
Selvam
S
Thavaraj
Julian R
JR
Naglik
Stephen J
SJ
Challacombe
D000818:Animals; D002176:Candida albicans / Q000254:growth & development* / Q000472:pathogenicity*; D002180:Candidiasis, Oral / Q000150:complications* / Q000382:microbiology* / Q000473:pathology; D002181:Candidiasis, Vulvovaginal / Q000150:complications* / Q000382:microbiology* / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D054884:Host-Pathogen Interactions; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA