Synthesis of N-methylated cyclic peptides.
This protocol presents a detailed description of the synthesis of N-methylated cyclic peptides. N-methylation is a powerful technique to modulate the physicochemical properties of peptides by introducing one or more methyl groups into the peptidic amide bonds. Together with peptide cyclization, this procedure confers unprecedented pharmacokinetic properties to the peptides, including metabolic stability, membrane permeability and even oral bioavailability. Here we describe two simplified methods of N-methylation of linear peptides on solid supports, which can be performed in less than 2 h and are applicable to any amino acid. Finally, we also describe two methods of peptide cyclization, which can be used to obtain the N-methylated cyclic peptide and which are not limited to specific peptide sequences. With this protocol, multiply N-methylated cyclic peptides can be synthesized in as little as 4-5 d.
Institute for Advanced Study and Center for Integrated Protein Science at the Department Chemie, Technische Universität München, Garching, Germany.
Jayanta
J
Chatterjee
Burkhardt
B
Laufer
Horst
H
Kessler
D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D003500:Cyclization; D013058:Mass Spectrometry; D008745:Methylation; D015394:Molecular Structure; D010456:Peptides, Cyclic / Q000138:chemical synthesis* / Q000737:chemistry / Q000493:pharmacokinetics
D010456:Peptides, Cyclic