A high molecular weight protein extract of Mastobranchus indicus (Mi-64) having antiarthritic activity in experimental animals.
Mi-64, a high molecular weight protein (130 kDa), obtained from the tissue homogenate of marine polychaete (Mastobranchus indicus) collected from the Indian Sunderban has antiarthritic activity in experimental animals. The FCA-induced arthritis model was developed in Wistar albino rats to evaluate the antiarthritic effects of Mi-64. After FCA induction, the rats were treated with Mi-64 (0.25 and 0.5 mg kg(-1) body weight) for 10 days. We have determined the paw/ankle swellings, urinary hydroxyproline and glucosamine, serum acid and alkaline phosphatases to assess the antiarthritic activity. The levels of interleukin-1 beta (IL-1β), IL-6, cytokine-induced neutrophil chemoattractant-1 (CINC-1), tumor necrosis factor-alpha (TNF-α), and IL-10 were measured by ELISA. Results showed that Mi-64 significantly reduced paw/ankle swellings and restored the urinary hydroxyproline/glucosamine and serum phosphatases. Mi-64 significantly inhibited the overproduction of IL-1β, IL-6, CINC-1, and TNF-α and augmented IL-10 production. The data suggest that Mi-64 produced significant antiarthritic effects that may be mediated by balancing the pro- and anti-inflammatory cytokines.
Department of Marine Science, University of Calcutta, 35, Ballygunge Circular Road, Kolkata, 700019, India. alam.mohammedaftab@gmail.com
Mohammed Aftab
MA
Alam
Santosh Kumar
SK
Sarkar
Antony
A
Gomes
D000135:Acid Phosphatase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D000843:Ankle Joint / Q000187:drug effects / Q000473:pathology; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology / Q000627:therapeutic use*; D001169:Arthritis, Experimental / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism*; D054360:Chemokine CXCL1 / Q000097:blood; D004195:Disease Models, Animal; D005944:Glucosamine / Q000652:urine; D006909:Hydroxyproline / Q000652:urine; D016753:Interleukin-10 / Q000097:blood; D053583:Interleukin-1beta / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D011077:Polychaeta; D011506:Proteins / Q000302:isolation & purification* / Q000494:pharmacology / Q000627:therapeutic use*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
D000894:Anti-Inflammatory Agents, Non-Steroidal; D054360:Chemokine CXCL1; C513297:Cxcl1 protein, rat; D053583:Interleukin-1beta; D015850:Interleukin-6; D011506:Proteins; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D005944:Glucosamine; D006909:Hydroxyproline