[Effect of PRX-2 gene transferred by lipofectamine on the proliferation of human skin fibroblasts].
OBJECTIVE
To explore the effects of PRX-2 gene transferred by lipofectamine on the human skin fibroblasts.


METHODS
Normal human skin fibroblasts were cultured and PRX-2 gene was transferred by lipofectamine. The proliferation of fibroblasts was detected by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) and flow cytometry.


RESULTS
The proliferation of PRX-2-transfected fibroblasts was stronger than that of normal counterparts. There were fewer cells during G0-G1 period and more cells during S and G2-M periods. The proliferative index increased.


CONCLUSIONS
The proliferation of fibroblasts may be modified by transfected PRX-2. Thus PRX-2 plays an important role during the healing of human skin wound.
Department of Burns & Plastic Surgery, First Affiliated Hospital, PLA General Hospital, Burns Institute of PLA, Beijing 100048, China.
Hui-feng
HF
Song
Jia-ke
JK
Chai
Zi-hao
ZH
Lin
D000293:Adolescent; D000328:Adult; D049109:Cell Proliferation; D002478:Cells, Cultured; D002648:Child; D005260:Female; D005347:Fibroblasts / Q000166:cytology*; D005801:Genes, Homeobox; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D008081:Liposomes; D008297:Male; D012867:Skin / Q000166:cytology; D014162:Transfection; D014945:Wound Healing; D055815:Young Adult
D018398:Homeodomain Proteins; D008081:Liposomes; C512693:PRRX2 protein, human