Assays for aptamer-based platforms.
Aptamers are single stranded DNA or RNA oligonucleotides that have high affinity and specificity towards a wide range of target molecules. Aptamers have low molecular weight, amenable to chemical modifications and exhibit stability undeterred by repetitive denaturation and renaturation. Owing to these indispensable advantages, aptamers have been implemented as molecular recognition element as alternative to antibodies in various assays for diagnostics. By amalgamating with a number of methods that can provide information on the aptamer-target complex formation, aptamers have become the elemental tool for numerous biosensor developments. In this review, administration of aptamers in applications involving assays of fluorescence, electrochemistry, nano-label and nano-constructs are discussed. Although detection strategies are different for various aptamer-based assays, the core of the design strategies is similar towards reporting the presence of specific target binding to the corresponding aptamers. It is prognosticated that aptamers will find even broader applications with the development of new methods of transducing aptamer target binding.
Infectious Disease Cluster, Advanced Medical & Dental Institute (AMDI), Universiti Sains Malaysia, 13200 Kepala Batas, Penang, Malaysia.
Marimuthu
M
Citartan
Subash C B
SC
Gopinath
Junji
J
Tominaga
Soo-Choon
SC
Tan
Thean-Hock
TH
Tang
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001681:Biological Assay / Q000379:methods*; D015374:Biosensing Techniques; D055664:Electrochemical Techniques / Q000379:methods*; D005453:Fluorescence; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry
D052157:Aptamers, Nucleotide