Cloning, characterization, and expression analysis of a novel BmGDAP1 gene from silkworm, Bombyx mori, involved in cytoplasmic polyhedrosis virus infection.
A novel ganglioside-induced differentiation-associated protein 1 gene (BmGDAP1) was first cloned and sequenced from silkworm, Bombyx mori using rapid amplification of cDNA ends (RACE). The full-length cDNA of BmGDAP1 was 1514bp, consisting of a 91bp 5' untranslated region (UTR), a 424bp 3'-UTR and a 999bp open reading frame (ORF). The ORF encoded a polypeptide of 332 amino acids, which possessed a thioredoxin (TRX)-like domain, a glutathione S-transferase-C (GST-C) family domain and a transmembrane segment. Furthermore, quantitative real-time PCR analysis revealed that BmGDAP1 transcripts were mainly presented in the tissues of hemocytes and midgut of silkworm, and its expression level was down-regulated in the hemocytes, while up-regulated in the midgut. Therefore, it could be concluded that BmGDAP1 plays an important role in the recognition and immune response of silkworm to BmCPV infection.
Sericultural Research Institute, Jiangsu University of Science and Technology, Jiangsu, China.
Kun
K
Gao
Xiangyuan
X
Deng
Heying
H
Qian
Ping
P
Wu
Guangxing
G
Qin
Xijie
X
Guo
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000818:Animals; D012831:Bombyx / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D003001:Cloning, Molecular / Q000379:methods; D018076:DNA, Complementary / Q000235:genetics; D015536:Down-Regulation / Q000235:genetics / Q000276:immunology; D005091:Exons; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D006434:Hemocytes / Q000276:immunology / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D016366:Open Reading Frames; D058974:Phylogeography / Q000379:methods; D017434:Protein Structure, Tertiary; D012087:Reoviridae / Q000276:immunology*; D012088:Reoviridae Infections / Q000276:immunology*; D017422:Sequence Analysis, DNA / Q000379:methods; D013879:Thioredoxins / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics; D015854:Up-Regulation
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D018076:DNA, Complementary; C118663:GDAP protein; D009419:Nerve Tissue Proteins; D013879:Thioredoxins; D005982:Glutathione Transferase