Assessing the anti-tumour properties of Iraqi propolis in vitro and in vivo.
The study was designed to evaluate anti-tumour properties of Iraqi propolis collected from Mosul region (M) on HL-60 and HCT-116 cell lines and on HCT-116 in vivo. M induced an inhibitory effect against the proliferation of HL-60 and colony potential of HCT-116 cells. The apoptosis in HL-60 cells was associated with down-regulation of Bcl-2 and activation of Bax, while in HCT-116 cells, necrotic features were observed; size of cells was dramatically increased by swelling of cytoplasm and loss of membrane integrity, cell rupture and release of cellular contents. Analysis of BrdU/DNA cell cycle in both cell lines showed that M induced cell cycle perturbations in both BrdU positive and BrdU negative cells. The exposure of HL-60 to M caused γ-H2AX in a dose dependent manner and was associated with induction of apoptosis. The experiments in HCT-116 tumor-bearing mice showed that oral administration of propolis at doses that caused no detectable toxicity was associated with a decrease in mitotic cells and an increase in endoreduplications, increased p53 and decreased Ki-67 expression of cells in tumor sections. This study provides the rationale to investigate the potential beneficial effect of propolis in the diet of patients receiving anti-cancer therapies.
Department of Applied Sciences, Division of Biotechnology, University of Technology, Baghdad, Iraq.
Ghassan M
GM
Sulaiman
Ali H
AH
Ad'hiah
Khulood W
KW
Al-Sammarrae
Renzo
R
Bagnati
Roberta
R
Frapolli
Ezia
E
Bello
Sarah
S
Uboldi
Michela
M
Romano
Nicolò
N
Panini
Eugenio
E
Scanziani
Marzia
M
Pezzolato
Eugenio
E
Erba
Maurizio
M
D'Incalci
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D007493:Iraq; D051379:Mice; D008819:Mice, Nude; D008856:Microscopy, Fluorescence; D009369:Neoplasms / Q000378:metabolism / Q000473:pathology*; D011429:Propolis / Q000494:pharmacology*; D051028:bcl-2-Associated X Protein / Q000378:metabolism
D000970:Antineoplastic Agents; D051028:bcl-2-Associated X Protein; D011429:Propolis