A teleostean angiotensinogen from Oplegnathus fasciatus responses to immune and injury challenges.
Angiotensinogen (AGT) is the precursor of the renin-angiotensin system and contributes to osmoregulation, acute-phase and immune responses. A full-length cDNA of the AGT (2004 bp with a 1389 bp coding region) was isolated from rock bream (Rb), Oplegnathus fasciatus. The encoded polypeptide of 463 amino acids had a predicted molecular mass of 51.6 kDa. RbAGT possessed a deduced signal peptide of 22 residues upstream of a putative angiotensin I sequence ((23)NRVYVHPFHL(32)). RbAGT possessed a specific domain profile and a signature motif which are characteristics of the serpin family. Sequence homology and phylogenetic analysis indicated that RbAGT was evolutionarily closest to AGT of Rhabdosargus sarba. The mRNA expression profile of RbAGT was determined by quantitative RT-PCR and it demonstrated a constitutive and tissue-specific expression with the highest transcript level in the liver. Significantly up-regulated RbAGT expression was elicited by systemic injection of a lipopolysaccharide, rock bream iridovirus (RBIV) and bacteria (Edwardsiella tarda and Streptococcus iniae), revealing its pathogen inducibility. RbAGT manifested a down-regulated response to systemic injury, contemporaneously with two other serpins, protease nexin-1 (PN-1), and heparin cofactor II (HCII). In addition, a synchronized expression pattern was elicited by RbAGT and RbTNF-α in response to injury, suggesting that TNF-α might be a potential modulator of AGT transcription.
Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University, Ara-Dong, Jeju Special Self-Governing Province, Republic of Korea.
Navaneethaiyer
N
Umasuthan
Ilson
I
Whang
Kasthuri Saranya
KS
Revathy
Myung-Joo
MJ
Oh
Sung-Ju
SJ
Jung
Cheol Young
CY
Choi
Jeong-Ho
JH
Lee
Jae Koo
JK
Noh
Jehee
J
Lee
D000808:Angiotensinogen / Q000235:genetics* / Q000276:immunology*; D000818:Animals; D003001:Cloning, Molecular; D004266:DNA Virus Infections / Q000276:immunology / Q000662:veterinary; D020609:Edwardsiella tarda / Q000502:physiology; D004756:Enterobacteriaceae Infections / Q000276:immunology / Q000662:veterinary; D005393:Fish Diseases / Q000276:immunology; D017994:Iridovirus / Q000502:physiology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008969:Molecular Sequence Data; D010473:Perciformes / Q000235:genetics* / Q000276:immunology*; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D017421:Sequence Analysis / Q000662:veterinary; D017385:Sequence Homology; D013290:Streptococcal Infections / Q000276:immunology / Q000662:veterinary; D013291:Streptococcus / Q000502:physiology
D008070:Lipopolysaccharides; D000808:Angiotensinogen