Laboratory diagnosis of tuberculosis in a large pediatric hospital in Cambodia.
METHODS
Tuberculosis laboratory in the Jayavarman VII Children's Hospital in Siem Reap, part of the Kantha Bopha Hospitals, the largest pediatric hospital complex in Cambodia.


OBJECTIVE
To determine the efficiency of on-site microscopy and rRNA amplification in children with a clinical diagnosis of tuberculosis (TB) and specimen sampling for culture.


RESULTS
From 1 July 2005 to 31 March 2006, 52,400 children were admitted to the hospital. Among these, 405 children had tuberculosis, including 91 (22.5%) laboratory-confirmed cases, or respectively 7.7 and 1.7 per 1000 admissions. Among cases confirmed by microscopy or rRNA assay, rRNA identified 91.2%. Among all culture-confirmed cases, rRNA identified 90.5%. Culture alone contributed 7.1% to all laboratory confirmed cases. The yield of culture from preserved specimens was not affected by shipment delay. For 97.4% of the children, the maximum turnaround time for the on-site laboratory result was 48 h.


CONCLUSIONS
Implementation of a mycobacteriology service in a referral hospital is feasible, as the molecular technique is highly efficient. Storage of specimen aliquots allows subsequent culture without loss of viability due to shipment delay.
Institute of Infectious Diseases, University of Berne, Switzerland. kurt.schopfer@bluewin.ch
K
K
Schopfer
H L
HL
Rieder
T
T
Bodmer
J F
JF
Steinlin-Schopfer
Y
Y
Chantana
T
T
Somathea
P
P
Studer
D
D
Laurent
B
B
Richner
D000293:Adolescent; D001431:Bacteriological Techniques / Q000379:methods; D002160:Cambodia; D002648:Child; D002675:Child, Preschool; D019411:Clinical Laboratory Techniques; D005240:Feasibility Studies; D005260:Female; D006776:Hospitals, Pediatric; D006801:Humans; D007223:Infant; D007755:Laboratories, Hospital; D008297:Male; D008853:Microscopy / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013048:Specimen Handling; D013997:Time Factors; D014376:Tuberculosis / Q000175:diagnosis* / Q000382:microbiology
D012336:RNA, Ribosomal, 16S