A novel xenograft model with intrinsic vascularisation for growing undifferentiated pleomorphic sarcoma NOS in mice.
OBJECTIVE
Preclinical development of antisarcoma therapy is primarily based on the subcutaneous transplantation of sarcoma xenografts. Tumour cell survival remains a hurdle of current models, which has been attributed to the hypoxic conditions following transplantation. We hypothesised that sarcoma models with an intrinsic tissue-engineered vascular supply are easily reproducible. The aim of this study was to establish a novel vascularised xenograft model.


METHODS
Primary human soft tissue sarcomas were transplanted into a silicon chamber and placed around the superficial epigastric vessels of nude mice. Sarcoma xenograft samples were assessed histomorphologically.


RESULTS
All sarcoma xenografts engrafted, leading to solid tumours. Histological, immunohistochemical staining and light/electron microscopy confirmed the xenografts as identical high-grade pleomorphic sarcomas (NOS) compared with the original patients' tumours.


CONCLUSIONS
This novel sarcoma xenograft model with an intrinsic vascular supply could be of high value for studying human soft tissue sarcomas and their therapy.
Department of Plastic Surgery, Reference Centre for Soft Tissue Sarcoma, BG University Hospital Bergmannsheil, Ruhr-University Bochum, Bochum, North Rhine-Westphalia, Germany. d.tilkorn@web.de
Daniel-Johannes
DJ
Tilkorn
Adrien
A
Daigeler
Joerg
J
Hauser
Andrej
A
Ring
Ingo
I
Stricker
Inge
I
Schmitz
Lars
L
Steinstraesser
Hans-Ulrich
HU
Steinau
Sammy
S
Al-Benna
D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D004195:Disease Models, Animal; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008954:Models, Biological; D009368:Neoplasm Transplantation; D009389:Neovascularization, Pathologic / Q000473:pathology*; D012509:Sarcoma / Q000098:blood supply / Q000473:pathology*; D012983:Soft Tissue Neoplasms / Q000098:blood supply / Q000473:pathology*; D014183:Transplantation, Heterologous