Screening of antigen-specific antibody-secreting cells.
Screening of antigen-specific antibody-producing cells is a key step for obtaining antigen-specific monoclonal antibodies. In murine system, hybridoma between B-lymphocytes and myeloma cells is used to screen and produce antigen-specific monoclonal antibodies. In human system, good hybridoma-producing system is not available. Instead, transformation of B-lymphocytes with Epstein-Barr viruses is used to obtain antibody-secreting cell lines. Furthermore, phage-display system using molecular biology is recently used to obtain antigen-specific human monoclonal antibodies. Here, we describe the new method for screening antigen-specific antibody-secreting cells at single-cell levels using microwell-array chips. The system can be applied to screen antigen-specific antibody-secreting cells from any animal species.
Department of Immunology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan. immkishi@med.u-toyama.ac.jp
Hiroyuki
H
Kishi
Aishun
A
Jin
Tatsuhiko
T
Ozawa
Kazuto
K
Tajiri
Tsutomu
T
Obata
Atsushi
A
Muraguchi
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000921:Antibody-Producing Cells / Q000276:immunology*; D000941:Antigens / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D045744:Cell Line, Tumor; D002472:Cell Transformation, Viral; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D046888:Tissue Array Analysis / Q000379:methods*
D000911:Antibodies, Monoclonal; D000941:Antigens