[The relationship between oxidative injury induced by low glucose and mitochondrial membrane potential in HUVEC-12 cells].
OBJECTIVE
To investigate the relationship between the oxidative injury induced by low glucose and mitochondrial membrane potential in HUVEC-12 cells.


METHODS
Human umbilical vein endothelial cells HUVEC-12 were cultured in low concentration glucose for 4 h. Cell viability of HUVEC-12 cell was assessed with MTT assay. Dihydroethidium (DHE) was used as a reactive oxygen species (ROS) capture, which was detected the mean fluorescence intensity of samples and Rhodamine 123 as a fluorescence detector was to measure the level of mitochondrial membrane potential (MMP) in cells.


RESULTS
Comparing to HUVEC-12 cells viability in 5.5 mmol/L glucose group (96.80 ± 3.20)%, cells exposed to 2.8 mmol/L glucose group (66.40 ± 1.60)% and 0 mmol/L glucose group (58.93 ± 1.67)% were decreased by 32% and 40% respectively (P < 0.01). ROS level of 5.5 mmol/L glucose group, 2.8 mmol/L glucose group and 0 mmol/L glucose group were 0.59 ± 0.02, 0.74 ± 0.04 and 0.88 ± 0.05, respectively, increased by 25% in cells exposed to 2.8 mmol/L glucose and by 48% in cells without glucose exposure comparing to 5.5 mmol/L glucose group (P < 0.01); MMP levels of 5.5 mmol/L glucose group, 2.8 mmol/L glucose group and 0 mmol/L glucose group were 148.83 ± 3.51, 271.07 ± 19.54 and 357.74 ± 51.32 respectively, increased to 1.8 times in cells exposed to 2.8 mmol/L glucose and to 2.4 times in cells without glucose exposure comparing to 5.5 mmol/L glucose group (P < 0.01).


CONCLUSIONS
Low glucose leads to injury in HUVEC-12 cells, which is probably induced by the oxidative stress via the increasing MMP.
Department of Endocrinology, Southern Medical University, Guangzhou, China.
Wen
W
Lu
Yao-ming
YM
Xue
Bo
B
Zhu
Xin
X
Lian
Ning
N
Liu
D002478:Cells, Cultured; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects*; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism*
D017382:Reactive Oxygen Species; D005947:Glucose