Radial sizing of lipid nanotubes using membrane displacement analysis.
We report a novel method for the measurement of lipid nanotube radii. Membrane translocation is monitored between two nanotube-connected vesicles, during the expansion of a receiving vesicle, by observing a photobleached region of the nanotube. We elucidate nanotube radii, extracted from SPE vesicles, enabling quantification of membrane composition and lamellarity. Variances of nanotube radii were measured, showing a growth of 40-56 nm, upon increasing cholesterol content from 0 to 20%.
Department of Chemical and Biological Engineering, Chalmers University of Technology, SE-41296 Göteborg, Sweden.
Natalia
N
Stepanyants
Gavin D M
GD
Jeffries
Owe
O
Orwar
Aldo
A
Jesorka
D036681:Fluorescence Recovery After Photobleaching / Q000379:methods*; D008055:Lipids / Q000737:chemistry*; D046911:Macromolecular Substances / Q000737:chemistry; D008422:Materials Testing; D008567:Membranes, Artificial; D008968:Molecular Conformation; D009038:Motion; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure*; D010316:Particle Size; D013499:Surface Properties
D008055:Lipids; D046911:Macromolecular Substances; D008567:Membranes, Artificial