Profile of circulating levels of IL-1Ra, CXCL10/IP-10, CCL4/MIP-1β and CCL2/MCP-1 in dengue fever and parvovirosis.
Dengue virus (DENV) and parvovirus B19 (B19V) infections are acute exanthematic febrile illnesses that are not easily differentiated on clinical grounds and affect the paediatric population. Patients with these acute exanthematic diseases were studied. Fever was more frequent in DENV than in B19V-infected patients. Arthritis/arthralgias with DENV infection were shown to be significantly more frequent in adults than in children. The circulating levels of interleukin (IL)-1 receptor antagonist (Ra), CXCL10/inducible protein-10 (IP-10), CCL4/macrophage inflammatory protein-1 beta and CCL2/monocyte chemotactic protein-1 (MCP-1) were determined by multiplex immunoassay in serum samples obtained from B19V (37) and DENV-infected (36) patients and from healthy individuals (7). Forward stepwise logistic regression analysis revealed that circulating CXCL10/IP-10 tends to be associated with DENV infection and that IL-1Ra was significantly associated with DENV infection. Similar analysis showed that circulating CCL2/MCP-1 tends to be associated with B19V infection. In dengue fever, increased circulating IL-1Ra may exert antipyretic actions in an effort to counteract the already increased concentrations of IL-1β, while CXCL10/IP-10 was confirmed as a strong pro-inflammatory marker. Recruitment of monocytes/macrophages and upregulation of the humoral immune response by CCL2/MCP-1 by B19V may be involved in the persistence of the infection. Children with B19V or DENV infections had levels of these cytokines similar to those of adult patients.
Laboratório de Imunologia Viral, Instituto Oswaldo Cruz-Fiocruz, Rio de Janeiro, Brasil. lpinto@ioc.fiocruz.br
Luzia Maria
LM
de-Oliveira-Pinto
Mariana
M
Gandini
Laís Picinini
LP
Freitas
Marilda Mendonça
MM
Siqueira
Cíntia Ferreira
CF
Marinho
Sérgio
S
Setúbal
Claire Fernandes
CF
Kubelka
Oswaldo Gonçalves
OG
Cruz
Solange Artimos de
SA
Oliveira
D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D018932:Chemokine CCL2 / Q000097:blood* / Q000276:immunology; D054407:Chemokine CCL4 / Q000097:blood* / Q000276:immunology; D054357:Chemokine CXCL10 / Q000097:blood* / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D003715:Dengue / Q000097:blood* / Q000276:immunology; D005260:Female; D006801:Humans; D007118:Immunoassay; D007223:Infant; D007231:Infant, Newborn; D053590:Interleukin 1 Receptor Antagonist Protein / Q000097:blood* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D010322:Parvoviridae Infections / Q000097:blood* / Q000276:immunology; D011446:Prospective Studies; D055815:Young Adult
D015415:Biomarkers; D018932:Chemokine CCL2; D054407:Chemokine CCL4; D054357:Chemokine CXCL10; D053590:Interleukin 1 Receptor Antagonist Protein