DNA template-assisted modulation of horseradish peroxidase activity.
The precise alignment of DNA molecules by Watson-Crick base-pairing combined with its polymeric characteristics have allowed DNA to be used as a template or scaffold for assembling materials. In this work, we investigate the role of calf-thymus DNA as a template for enhancing the horseradish peroxidase (HRP)-mediated oxidation of phenol and phenolic derivatives. The HRP-catalyzed oxidation of phenol into polyphenolic products and in presence of 4-aminoantipyrine into quinoneimine dye complexes is studied. Visible spectroscopy reveals an increased yield of both products of the enzymatic reaction in presence of calf-thymus DNA and is attributed to the prearrangement of the corresponding substrates on the DNA. The concentrations of calf-thymus DNA and the substrates are found to affect the nature of prearrangement and subsequent formation of polymeric or co-oxidation products. Also, phenolic derivatives with different aromatic substitutions display divergent propensities towards product formation in presence of the DNA template. Our results demonstrate the ability of calf-thymus DNA to modulate the activity of HRP and exercise control on the nature of products formed. This work highlights the potential of using DNA as a template for influencing enzymatic reactions involving aromatic substrates.
Department of Chemistry, Missouri State University, 901 S. National Avenue, Springfield, MO 65897, USA.
Shohini Ghosh
SG
Datta
Xiaozheng
X
Dou
Alyxandria
A
Shibley
Bhaskar
B
Datta
D000675:Ampyrone / Q000378:metabolism; D000818:Animals; D020029:Base Pairing; D002417:Cattle; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D006735:Horseradish Peroxidase / Q000378:metabolism*; D010084:Oxidation-Reduction; D010636:Phenols / Q000378:metabolism; D013379:Substrate Specificity
D010636:Phenols; D000675:Ampyrone; D004247:DNA; D006735:Horseradish Peroxidase