Alginate films containing viable Lactobacillus plantarum: preparation and in vitro evaluation.
The objective of the study was to develop calcium alginate films, containing Lactobacillus plantarum ATCC 8040 with preserved and stable viability and antibacterial activity. L. plantarum-loaded films containing different calcium concentrations were physically characterized for mechanical and bioadhesive properties and lactobacilli release. The viability and antibacterial activity of L. plantarum was studied before and after processing, and during 6 months of storage. A multiresistant clinical isolate, VIM-2-metalo-β-lactamase producing Pseudomonas aeruginosa, was used as an indicator strain. Interference between L. plantarum and films enhanced films elasticity, water absorption ability, release of lactobacilli, and decreased films adherence. A decrease of L. plantarum viability in alginate films (≤1 log unit) was observed after freeze drying. L. plantarum, at cell concentrations of 108 cfu/ml, was inhibitory active. The viability and antibacterial activity of the immobilized lactobacilli remained stable during 6 months of storage. The study has proved the potential of alginate films to deliver L. plantarum in high numbers to individuals.
i-Med.UL Research Institute for Medicines and Pharmaceutical Sciences, Faculdade de Farmácia da Universidade de Lisboa, Av. Prof. Gama Pinto, P-1640-003 Lisbon, Portugal.
Mariya I
MI
Brachkova
Aida
A
Duarte
João F
JF
Pinto
D000268:Adhesiveness; D000464:Alginates / Q000737:chemistry*; D000818:Animals; D002645:Chickens; D004304:Dosage Forms; D024901:Drug Resistance, Multiple, Bacterial; D055119:Elastic Modulus; D005612:Freeze Drying; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D048191:Lactiplantibacillus plantarum / Q000254:growth & development*; D050296:Microbial Viability; D019936:Probiotics / Q000008:administration & dosage / Q000737:chemistry*; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000254:growth & development; D012867:Skin / Q000737:chemistry; D013499:Surface Properties; D013718:Tensile Strength; D013997:Time Factors; D001618:beta-Lactamases / Q000378:metabolism
D000464:Alginates; D004304:Dosage Forms; D006603:Hexuronic Acids; D020723:Glucuronic Acid; C425108:beta-lactamase bla(vim-2); D001618:beta-Lactamases