Association between circulating full-length osteopontin and IFN-gamma with disease status of tuberculosis and response to successful treatment.
The T helper type 1 (Th1) immune response plays an important role in protective immunity, pathophysiology and development of tuberculosis (TB). To investigate whether osteopontin (OPN) and other Th1 response-related molecules are associated withTB disease status, including co-infection with HIV, and response to anti-TB treatment, circulating levels of full-length OPN (F-OPN), thrombin-cleaved N-terminal fragment of OPN (N-half OPN), IFN-gamma, IP-10, IL-18, IL-12/ IL-23 (p40), IL-10, IL-15 and C-reactive protein (CRP) were measured before and after anti-TB treatment. Patients with newly active pulmonary TB had significantly higher plasma levels of F-OPN, IFN-gamma and CRP than healthy controls (HC). F-OPN, N-half OPN, IFN-gamma, IP-10, IL-18 and IL-10 levels were higher in patients with extensive TB/HIV co-infection than in patients with a single disease of TB or HIV. Plasma levels of F-OPN correlated well with those of IP-10, IL-18 and N-half OPN among patients with active TB. The F-OPN, IFN-gamma, IP-10 and CRP levels decreased significantly after effective anti-TB treatment. These data suggest that circulating OPN and Th1 response-related molecules, including IFN-gamma, may be regulated in response to expansion of active TB and could serve as markers of disease activity before and during treatment.
Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Chutharut
C
Ridruechai
Shinsaku
S
Sakurada
Hideki
H
Yanai
Norio
N
Yamada
Pacharee
P
Kantipong
Surachai
S
Piyaworawong
Panadda
P
Dhepakson
Srisin
S
Khusmith
Naoto
N
Keicho
D000328:Adult; D023241:Antiretroviral Therapy, Highly Active; D000995:Antitubercular Agents / Q000627:therapeutic use; D002097:C-Reactive Protein / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism; D054357:Chemokine CXCL10 / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015658:HIV Infections / Q000097:blood* / Q000188:drug therapy; D006801:Humans; D007371:Interferon-gamma / Q000097:blood*; D007378:Interleukins / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D053495:Osteopontin / Q000097:blood*; D012698:Serologic Tests; D013785:Thailand; D014397:Tuberculosis, Pulmonary / Q000097:blood* / Q000175:diagnosis* / Q000188:drug therapy
D000995:Antitubercular Agents; D054357:Chemokine CXCL10; D007378:Interleukins; D053495:Osteopontin; D007371:Interferon-gamma; D002097:C-Reactive Protein