Lipidomics.
OBJECTIVE
Lipidomics characterizes the composition of intact lipid molecular species in biological systems and the field has been driven by some spectacular advances in mass spectrometry instrumentation and applications. This review will highlight these advances and outline their recent application to address clinical issues.


RESULTS
This review first identifies recent advances in lipid detection and analysis by a variety of mass spectrometry techniques, then reviews specific application including stable isotope labelling of lipids, lipid mass spectrometry imaging, data analysis and bioinformatics, and finally presents examples of the application of lipidomics to selected disease states.


CONCLUSIONS
Lipidomics so far has been principally concerned with identifying novel methodologies, but recent advances demonstrating applications in diabetes, neurodegenerative diseases, cystic fibrosis and other respiratory diseases clearly indicate the potential usefulness of lipidomics both to generate biomarkers of disease and to probe signalling and metabolic processes.
Clinical and Experimental Sciences, University of Southampton, Hampshire, UK. adp@soton.ac.uk
Anthony D
AD
Postle
D015415:Biomarkers / Q000032:analysis / Q000737:chemistry; D019295:Computational Biology / Q000379:methods; D006801:Humans; D007553:Isotope Labeling; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis* / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D015415:Biomarkers; D008055:Lipids