[Effect of superantigen SEA on mitochondrial membrane potential of Molt-4 cell].
OBJECTIVE
To explore the effect of superantigen staphylococcal enterotoxin A(SEA) on mitochondrial membrane potential of Molt-4 cell.


METHODS
Cell counting kit-8(CCK-8) was used to detect the proliferation of T cell in different concentration and time,  We employed JC-1 to estimate mitochondrialmembrane potential (δψm) of Molt-4 cell induced by SEA using flow cytometry (FCM).


RESULTS
The proliferative activity of T cell treated with SEA(1 mg/L) was changed obviously compared with control.The mitochondrial membrane potential of Molt-4 cell with SEA(1  mg/L) was highest at 10 min by FCM after stimulation of 180, 60, 30 and 10 min.   Mitochondrial membrane potential of Molt-4 cell treated with SEA after 10 and 30 min were lower than that with PHA which also have rising effect.


CONCLUSIONS
Superantigen can enhance the mitochondrial membrane potential of Molt-4 cell in the early stage of proliferation.But the effect was weaker than that with mitogen PHA.
Department of Microbiology and Immunology, Medical College, Jinan University, Guangzhou, China. qyn986221@sina.com
Ya-nan
YN
Qin
Hong-xia
HX
Tian
Guan-ming
GM
Wang
Peng
P
Zhang
Chen
C
Lin
Yang-qiu
YQ
Li
D002460:Cell Line; D004768:Enterotoxins / Q000276:immunology / Q000494:pharmacology*; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects*; D018089:Superantigens / Q000276:immunology / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology
D004768:Enterotoxins; D018089:Superantigens; C026066:enterotoxin A, Staphylococcal