Interaction of transient receptor potential vanilloid 4 with annexin A2 and tubulin beta 5.
The aim of the present study was to investigate if there exists an interaction of TRPV4 with annexin A2 and with tubulin beta 5 in transfected human embryonic kidney (HEK293) cells in vitro. Coimmunoprecipitation of the rat dorsal root ganglion was performed to validly conform the interaction of TRPV4 with the other two proteins. Gene fragments coding for the amino acids in protein were obtained. We conducted coimmunoprecipitation and immunofluorescence on the transfected cell samples. Coimmunoprecipitation experiments of transfected HEK293 cells revealed that TRPV4 and tubulin beta 5 associated together in a complex, whereas TRPV4 and annexin A2 did not. The immunofluorescence microscopy revealed a colocalization of TRPV4 with both the tubulin beta 5 and annexin A2. These results indicate an interaction between TRPV4 and tubulin beta 5 by associating together. However, the association between TRPV4 and annexin A2 may be mediated by some intermediate elements or just exists in some physiological conditions. Thus, TRPV4 channel function may be modulated by tubulin beta 5 and annexin A2 and their interactions may play a role in the mechanosensation in the pathogenesis of neuropathic pain.
Department of Physical Medicine & Rehabilitation, Qilu Hospital, Shandong University, Jinan 250012, China.
Juan
J
Huai
Yang
Y
Zhang
Qing-Mei
QM
Liu
Hong-You
HY
Ge
Lars
L
Arendt-Nielsen
Hong
H
Jiang
Shou-Wei
SW
Yue
D000818:Animals; D017306:Annexin A2 / Q000235:genetics / Q000378:metabolism*; D005727:Ganglia, Spinal / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D010957:Plasmids / Q000235:genetics; D051381:Rats; D050916:TRPV Cation Channels / Q000235:genetics / Q000378:metabolism*; D014404:Tubulin / Q000235:genetics / Q000378:metabolism*
D017306:Annexin A2; D050916:TRPV Cation Channels; C486577:Trpv4 protein, rat; D014404:Tubulin; C580942:beta5 tubulin, rat