Comparative analyses of protein complexes by blue native DIGE.
Classically, DIGE is carried out on the basis of two-dimensional (2D) IEF/SDS PAGE. This allows comparative analyses of large protein sets. However, 2D IEF/SDS PAGE only poorly resolves hydrophobic proteins and is not compatible with native protein characterizations. Blue native PAGE represents a powerful alternative. Combined with CyDye labeling, blue native DIGE offers several useful applications like quantitative comparison of protein complexes of related protein fractions. Here we present a protocol for fluorophore labeling of native protein fractions for separation by blue native PAGE.
Institute for Plant Genetics, Leibniz Universität Hannover, Hannover, Germany.
Katrin
K
Peters
Hans-Peter
HP
Braun
D011506:Proteins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D012394:Rosaniline Dyes; D057825:Two-Dimensional Difference Gel Electrophoresis / Q000379:methods*
D011506:Proteins; D012394:Rosaniline Dyes; C004692:coomassie Brilliant Blue