Bonding efficacy of an acetone/based etch-and-rinse  adhesive after dentin deproteinization.
OBJECTIVE
to evaluate the effect of sodium hypochlorite (NaOCl) treatment on dentin bonding by means of shear bond strength (SBS) measurements when using Prime&Bond NT (PB NT) adhesive. Ultrastructure of the interfaces was examined by scanning electron microscopy (SEM).


METHODS
Extracted human third molars were sectioned and ground to expose flat surfaces of superficial or deep dentin. Specimens were randomly assigned to two equal groups, and bonded as follows: (1) according to the manufacturers' directions, after 35% H₃PO₄ etching, (2) 5% NaOCl treated for 2 minutes, after 35% H₃PO₄ etching. Each sample was embedded in a Watanabe shear test assembly for a single plane lap shear. After PB NT bonding, specimens were stored in water for 24 h at 37ºC and thermocycled (500x). Samples were tested in shear to failure using a universal testing machine at 0.75 mm/min. Data were analyzed with ANOVA and Newman-Keuls multiple comparison test procedures. Two samples of each group were randomly selected to investigate the morphologic aspect of the resin/dentin interface with SEM.


RESULTS
After etching and after aqueous sodium hypochlorite (NaOClaq) application, SBS values were similar on superficial than deep dentin (p>0.05). SEM findings shows for H₃PO₄ etching conditioned samples a detectable hybrid layer and long resin tags; for NaOCl treated specimens, it may be observed a non apparent hybrid layer, and the adhesive contact directly with the neck of the cylindrical resin tags.


CONCLUSIONS
The use of 5% NaOCl for 2 min after dentin demineralization when PB NT was employed did not improve the bond strength to dentin, probably due to nanofiller content and/or oxidative changes on collagen-depleted dentin.
Department of Dental Materials, School of Dentistry, Campus de Cartuja 18071, University of Granada, Spain.
Fátima-Sánchez
FS
Aguilera
Raquel
R
Osorio
Estrella
E
Osorio
Pedro
P
Moura
Manuel
M
Toledano
D001840:Dental Bonding; D003804:Dentin / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D008422:Materials Testing; D008964:Molar, Third; D011109:Polymethacrylic Acids / Q000493:pharmacokinetics*; D012973:Sodium Hypochlorite / Q000494:pharmacology*
D011109:Polymethacrylic Acids; C425843:Prime and Bond NT; D012973:Sodium Hypochlorite