Extremely low frequency magnetic fields do not elicit oxidative stress in MCF10A cells.
The aim of this study was to determine whether extremely low frequency magnetic fields (ELF-MF) could affect intracellular reactive oxygen species (ROS) levels and antioxidant enzyme activity. After MCF10A human breast epithelial cells were exposed to 1 mT of 60 Hz ELF-MF for 4 hours, intracellular ROS level, superoxide dismutase (SOD) activity, and reduced to oxidized glutathione (GSH/GSSG) ratio were measured. The cells exposed to ELF-MF did not evidence statistically significant changes in the above-mentioned biological parameters as compared to either the incubator controls or sham-exposed cells. By way of contrast, the IR-exposed cells exhibited marked changes in ROS level, SOD activity, and GSH/GSSG ratio. When we assessed morphological changes and senescence-associated beta-galactosidase (SA-β-Gal) activity, only the IR-exposed cells were positive. According to our results, it could be concluded that ELF-MF has no effect on intracellular ROS level, SOD activity, and GSH/GSSG ratio under our exposure condition.
Division of Radiation Cancer Research, Korea Institute of Radiological & Medical Sciences, Seoul, Korea.
Mi-Na
MN
Hong
Na-Kyung
NK
Han
Hyung-Chul
HC
Lee
Young-Kyu
YK
Ko
Sung-Gil
SG
Chi
Yun-Sil
YS
Lee
Yoon-Myung
YM
Gimm
Sung-Ho
SH
Myung
Jae-Seon
JS
Lee
D000230:Adenocarcinoma / Q000473:pathology*; D001943:Breast Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor / Q000528:radiation effects; D048430:Cell Shape; D004847:Epithelial Cells / Q000378:metabolism / Q000528:radiation effects; D004867:Equipment Design; D005260:Female; D005720:Gamma Rays / Q000009:adverse effects; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D060526:Magnetic Fields / Q000009:adverse effects*; D009363:Neoplasm Proteins / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism; D012107:Research Design; D013482:Superoxide Dismutase / Q000378:metabolism
D009363:Neoplasm Proteins; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase; D005978:Glutathione