Overreliance on the hexon gene, leading to misclassification of human adenoviruses.
The genome of human adenovirus (HAdV) D30 was sequenced in depth. Sequence assembly and analysis revealed two distinct viral sequences with identical hexon genes, which were the same as the one previously reported for HAdV-D30. However, one of the two viruses was found to be a recombinant of HAdV-D29. Exclusive reliance on serum neutralization can lead to mischaracterization of adenoviruses and miss coinfections. Whole-genome sequencing remains the gold standard for proper classification of HAdVs.
Department of Ophthalmology, Howe Laboratory, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, Massachusetts, USA.
Gurdeep
G
Singh
Christopher M
CM
Robinson
Shoaleh
S
Dehghan
Timothy
T
Schmidt
Donald
D
Seto
Morris S
MS
Jones
David W
DW
Dyer
James
J
Chodosh
D000260:Adenoviruses, Human / Q000145:classification* / Q000235:genetics; D036022:Capsid Proteins / Q000235:genetics*; D002460:Cell Line; D019295:Computational Biology / Q000379:methods; D016679:Genome, Viral; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA
D036022:Capsid Proteins; C018315:hexon capsid protein, Adenovirus