Synchrotron radiation FTIR detection of a metal-carbonyl tamoxifen analog. Correlation with luminescence microscopy to study its subcellular distribution.
1,1-Di(4-hydroxyphenyl)-2-cyrhetrenylbut-1-ene 1 is an organometallic conjugate where a [(Cp)Re(CO)(3)] unit is linked to a hydroxytamoxifen-like structure. Its subcellular nuclear distribution was previously observed in a single cell using the near-field technique AFMIR. We show here that synchrotron radiation FTIR spectromicroscopy (SR-FTIR-SM) enabled the mapping of 1 based on its IR-signature (characteristic bands in the 1850-2200 cm(-1) range) and pointed out the colocalization of 1 with an area of high amide density. Fluorescence microscopy using DAPI staining performed on the same cells confirmed that this area corresponds to the cell nucleus.
Ecole Normale Supérieure, Département de chimie, 24, rue Lhomond, 75005 Paris, France.
Sylvain
S
Clède
François
F
Lambert
Christophe
C
Sandt
Zoher
Z
Gueroui
Nicolas
N
Delsuc
Paul
P
Dumas
Anne
A
Vessières
Clotilde
C
Policar
D001943:Breast Neoplasms / Q000378:metabolism; D045744:Cell Line, Tumor / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D005260:Female; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*; D009942:Organometallic Compounds / Q000032:analysis* / Q000493:pharmacokinetics; D010636:Phenols / Q000032:analysis* / Q000493:pharmacokinetics; D012211:Rhenium / Q000032:analysis; D013055:Spectrophotometry, Infrared / Q000379:methods; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D017356:Synchrotrons; D013629:Tamoxifen / Q000031:analogs & derivatives*
D009942:Organometallic Compounds; D010636:Phenols; D013629:Tamoxifen; D012211:Rhenium