Dried blood spot (DBS) sample collection for determination of the oxidative stress biomarker 8-epi-PGF(2α)  in humans using liquid chromatography/tandem mass spectrometry.
BACKGROUND
F2-isoprostanes are a series of prostaglandin F2-like compounds that are formed by free-radical-catalyzed peroxidation of arachidonic acid (ARA). Several F2-isoprostanes, but in particular 8-epi-PGF(2α), are widely used as oxidative stress biomarkers. In this study we have developed an analytical tool for finger-tip blood sampling and analysis of 8-epi-PGF(2α) from dried blood spots (DBS).


METHODS
We have applied solid-phase extraction (SPE) and liquid chromatography/tandem mass spectrometry (LC/MS/MS) for the extraction, separation and detection of 8-epi-PGF(2α) in DBS and have studied the stability of this marker using the DBS collection platform.


RESULTS
The mass limit of detection (mLOD) for 8-epi-PGF(2α) extracted from DBS samples was 1.5 pg while the concentration limit of detection (cLOD) and concentration limit of quantitation (cLOQ) were 6 pg/mL and 18 pg/mL, respectively. All values based on triplicate analysis. Sufficient stability of 8-epi-PGF(2α) in DBS was achieved by preconditioning DBS paper with vitamin E and BHT.


CONCLUSIONS
The developed method is sensitive, specific, robust, efficient, and can accurately measure endogenous concentrations of 8-epi-PGF(2α) in DBS. Thus, it offers an analytical approach to measure 8-epi-PGF(2α) by a novel sample collection technique that is less invasive and costly than conventional techniques.
Institute of Basic Medical Sciences, University of Oslo, P.O. Box 1046 Blindern, N-0316, Oslo, Norway. n.e.bastani@medisin.uio.no
Nasser E
NE
Bastani
Thomas E
TE
Gundersen
Rune
R
Blomhoff
D015415:Biomarkers / Q000097:blood; D002853:Chromatography, Liquid / Q000379:methods; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000097:blood; D006801:Humans; D057230:Limit of Detection; D018384:Oxidative Stress; D053719:Tandem Mass Spectrometry / Q000379:methods*
D015415:Biomarkers; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost