Perfusion culture of mammalian cells in a microfluidic channel with a built-in pillar array.
In vitro culture of mammalian cells is fundamental to various biological studies such as single cell analysis, pathological research, and drug/therapy development. However, there are limitations with the current in vitro cell culture methods. Cells tend to lose their specific functions due to the lack of a cellular microenvironment when they are maintained under standard culture conditions. Microscale devices can be a novel tool to reestablish a cellular microenvironment for culturing mammalian cells in vitro and maintaining their differentiated functions. Different microscale cell culture techniques have been developed to suit different biological applications. This chapter describes a method to trap and culture mammalian cells in a microfluidic channel with a built-in pillar array.
Division of Nanobiotechnology, AlbaNova University Centre, Royal Institute of Technology, Stockholm, Sweden. chizhang@kth.se
Chi
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D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002460:Cell Line; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004867:Equipment Design; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D055616:Microtechnology / Q000379:methods; D010477:Perfusion / Q000295:instrumentation / Q000379:methods; D012825:Silicon / Q000737:chemistry
D004129:Dimethylpolysiloxanes; D012825:Silicon