Enhancement of fermentative hydrogen production in an extreme-thermophilic (70°C) mixed-culture environment by repeated batch cultivation.
Repeated batch cultivation was applied to enrich hydrogen fermentative microflora under extreme-thermophilic (70°C) environment. Initial inoculums received from a hydrogen producing reactor fed with organic fraction of household solid wastes. In total seven transfers was conducted and maximum hydrogen yield reached 296 ml H(2)/g (2.38 mol/mol) glucose and 252 ml H(2)/g (2.03 mol/mol) for 1 and 2 g/l glucose medium, respectively. It was found that hydrogen production was firstly decreased and got increased gradually from third generation. Acetate was found to be the main metabolic by-product in all batch cultivation. Furthermore, the diversity of bacterial community got decreased after repeated batch cultivation. It was proved that repeated batch cultivation was a good method to enhance the hydrogen production by enriching the mixed cultures of dominant species.
School of Enviornment, Tsinghua University, Beijing 100084, China. luwenjing@tsinghua.edu.cn
Wenjing
W
Lu
Gaoyuan
G
Fan
Chenxi
C
Zhao
Hongtao
H
Wang
Zifang
Z
Chi
D001419:Bacteria / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D061249:Batch Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D019149:Bioreactors / Q000382:microbiology*; D003470:Culture Media / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006358:Hot Temperature; D006859:Hydrogen / Q000378:metabolism*
D003470:Culture Media; D006859:Hydrogen; D005947:Glucose