Comparative proteomic analysis of auxin-induced embryogenic and nonembryogenic tissues of the solanaceous tree Cyphomandra betacea (Tamarillo).
Cyphomandra betacea (tamarillo) is a tree that produces edible, highly nutritional fruits. In tamarillo, somatic embryogenesis (SE) is achieved through a two-step process starting with the formation of an embryogenic tissue on an auxin-rich medium and further development of embryos, following tissue transfer to an auxin-free medium. During the induction stage, both embryogenic (EC) and nonembryogenic calli (NEC) arise from the same explant (immature leaves or mature zygotic embryos) in the presence of either picloram or 2,4-dichlorophenoxyacetic acid. In an attempt to find somatic embryogenic-specific proteins, a comparative analysis of the proteome of tamarillo's EC and NEC was performed. Analysis of 2-DE gels revealed ca. 150 differentially expressed proteins, from which 22 have been identified by LC-MS/MS. Proteins exclusively or predominantly expressed in EC included metabolism-related proteins, such as enolases or treonine synthases, and also heat-shock and ribosomal proteins. Pathogenesis-related proteins were found mainly in NEC. A number of additional differentially expressed proteins involved in various functional categories were also identified. A quantitative real time PCR (qPCR) analysis revealed no significant differences at the mRNA level for 11 differentially expressed proteins, with exception of the pathogenesis-related proteins that were up-regulated in NEC. This seems to indicate that a posttranscriptional control might be responsible for the proteomic differences detected.
Center of Functional Ecology, Department of Life Sciences, University of Coimbra , Ap. 3046, 3001-401 Coimbra, Portugal. sandraimc@ci.uc.pt
Sandra
S
Correia
Raquel
R
Vinhas
Bruno
B
Manadas
Ana Sofia
AS
Lourenço
Paula
P
Veríssimo
Jorge M
JM
Canhoto
D015180:Electrophoresis, Gel, Two-Dimensional; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D007210:Indoleacetic Acids / Q000494:pharmacology*; D010449:Peptide Mapping; D010937:Plant Growth Regulators / Q000494:pharmacology*; D010940:Plant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D058439:Plant Somatic Embryogenesis Techniques / Q000379:methods*; D060066:Protein Interaction Maps; D020543:Proteome / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D040901:Proteomics; D032322:Solanum / Q000187:drug effects / Q000378:metabolism*; D014158:Transcription, Genetic
D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D010940:Plant Proteins; D020543:Proteome