[Expression,  purification and protective antigen analysis of cell wall protein MRP of Streptococcus suis type 2].
OBJECTIVE
To amplify the mrp gene of Streptococcus suis type 2 05ZYH33,  express it in E.coli BL21 in order to acquire high purity recombinant protein MRP,  then evaluate the protective antigen of recombinant protein MRP.


METHODS
Using PCR technology to obtain the product of mrp gene of 05ZYH33,  and then cloned it into the expression vector pET28a(+). The recombinant protein was purified by affinity chromatography,  later immunized New Zealand rabbit to gain anti-serum,  then test the anti-serum titer by ELISA. The opsonophagocytic killing test demonstrated the abilities of protective antigen of MRP.


RESULTS
The truncated of MRP recombinant protein in E.coli BL21 expressed by inclusion bodies,  and purified it in high purity. After immunoprotection,  the survival condition of CD-1 was significantly elevated. The survival rate of wild-type strain 05ZYH33 in blood was apparently decreased after anti-serum opsonophagocyticed,  but the mutant delta; MRP showed no differences.


CONCLUSIONS
MRP represent an important protective antigen activity.
Sports School, Shaanxi Normal University, Xi'an, China.
Ping-ping
PP
Wang
Ya-ya
YY
Pian
Yuan
Y
Yuan
Yu-ling
YL
Zheng
Yong-qiang
YQ
Jiang
Zheng-ying
ZY
Xiong
D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006801:Humans; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D013290:Streptococcal Infections / Q000276:immunology / Q000401:mortality / Q000517:prevention & control; D017011:Streptococcus suis / Q000235:genetics* / Q000276:immunology*
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C421489:MRP protein, Streptococcus suis; D011993:Recombinant Fusion Proteins