Analysis of single eukaryotic cells using Raman Tweezers.
Raman Tweezers is a technique that combines optical trapping with Raman spectroscopy and has enabled the spectroscopic analysis of single cells. Applications of this technique include the identification and discrimination of different types of cells, including healthy and non-healthy cells (e.g. cancer cells). In addition, the interaction of cells with stimuli, e.g. drugs, can also be studied on a single-cell basis. Herein, a generic protocol for the analysis of fixed and living single eukaryotic cells is described, including the considerations required to build a Raman Tweezers systems.
Manchester Interdisciplinary Biocentre, School of Chemical Engineering and Analytical Science, The University of Manchester, Manchester, UK. elsa.faria@manchester.ac.uk
Elsa Correia
EC
Faria
Peter
P
Gardner
D000818:Animals; D002470:Cell Survival; D004867:Equipment Design; D006801:Humans; D052898:Optical Tweezers; D025341:Principal Component Analysis; D059010:Single-Cell Analysis / Q000295:instrumentation* / Q000379:methods; D013059:Spectrum Analysis, Raman / Q000295:instrumentation* / Q000379:methods; D016707:Tissue Fixation / Q000379:methods