Anti-mitotic potential of 7-diethylamino-3(2'-benzoxazolyl)-coumarin in 5-fluorouracil-resistant human gastric cancer cell line SNU620/5-FU.
In this study, we investigate an anti-mitotic potential of the novel synthetic coumarin-based compound, 7-diethylamino-3(2'-benzoxazolyl)-coumarin, in 5-fluorouracil-resistant human gastric cancer cell line SNU-620-5FU and its parental cell SNU-620. It exerts the anti-proliferative effects with similar potencies against both cancer cells, which is mediated by destabilization of microtubules and subsequent mitotic arrest. Furthermore, this compound enhances caspase-dependent apoptotic cell death via decreased expression of anti-apoptotic genes. Taken together, our data strongly support anti-mitotic potential of 7-diethylamino-3(2'-benzoxazolyl)-coumarin against drug-resistant cancer cells which will prompt us to further develop as a novel microtubule inhibitor for drug-resistant cancer chemotherapy.
Department of Pharmacology, Kwandong University College of Medicine, Gangneung 210-701, Republic of Korea.
Nam Hyun
NH
Kim
Su-Nam
SN
Kim
Joa Sub
JS
Oh
Seokjoon
S
Lee
Yong Kee
YK
Kim
D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology*; D050256:Antimitotic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001583:Benzoxazoles / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003374:Coumarins / Q000494:pharmacology*; D019008:Drug Resistance, Neoplasm; D005472:Fluorouracil / Q000494:pharmacology*; D006801:Humans; D013274:Stomach Neoplasms / Q000188:drug therapy*; D050257:Tubulin Modulators / Q000494:pharmacology
C540953:7-diethylamino-3(2'-benzoxazolyl)coumarin; D000964:Antimetabolites, Antineoplastic; D050256:Antimitotic Agents; D001583:Benzoxazoles; D003374:Coumarins; D050257:Tubulin Modulators; D005472:Fluorouracil