[Effect and mechanisms of Shenmai injection on contractions of porcine coronary artery].
OBJECTIVE
To investigate the vasodilative action and the possible mechanisms of Shenmai injection on porcine coronary artery.


METHODS
Isometric tensions of the porcine coronary artery ring precontracted with potassium chloride (KCl) were recorded in vitro when the doses of 0.1, 0.5, 1, 5, 10, 50 mL x L(-1) of Shenmai injective were cumulatively added into the organ bath of porcine coronary artery ring.


RESULTS
Shenmai injection caused same vasorelaxation of porcine coronary artery rings with endothelium intact and denuded precontracted with KCl in a concentration-dependent manner. Pretreatment with TEA and 4-AP prohibited the vasorelaxation by Shenmai injection, but pretreatment with KB-R7943, BaCl2, Gli did not affect the vascular effect of Shenmai injection.


CONCLUSIONS
Shenmai injection could produce vasodilatation on KCl pre-contracted porcine coronary artery rings. It seems that K(Ca) and K(V) channel play an important role in the relaxation of the porcine coronary artery rings pre-contracted with KCl.
Pharmacy Department, First Hospital of Shanxi Medical University, Taiyuan 030001, China. 834575168@qq.com
Xiaojun
X
Zheng
Zhiqiang
Z
Hu
D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects / Q000502:physiology; D003331:Coronary Vessels / Q000187:drug effects* / Q000502:physiology / Q000503:physiopathology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism; D013552:Swine; D014664:Vasodilation / Q000187:drug effects; D014665:Vasodilator Agents / Q000494:pharmacology*
D004365:Drugs, Chinese Herbal; D014665:Vasodilator Agents; D009569:Nitric Oxide