[Prenatal exposure to lipopolysaccharide results in vascular renin-angiotensin system dysfunction in offspring rats].
OBJECTIVE
To explore the impact of prenatal exposure to lipopolysaccharide on renin-angiotensin system of offspring rats.


METHODS
Six pregnant SD rats were randomly divided into 2 groups. The rats in the lipopolysaccharide (LPS) group were treated with LPS 0.79 mg/kg (i.p.) on the 8th, 10th and 12th day of gestation, and rats in the control group were treated with saline at the same time points. The blood pressure of offspring rats was measured by the tail cuff method. Protein expression of Angiotensin II (AngII) in thoracic aorta vessel was determined by immunohistochemistry. Protein expressions of AngII type 1 and type 2 receptor in thoracic aorta vessel were detected by Western blot.


RESULTS
Blood pressure of 12-week-old offspring rats of LPS group was significantly higher than that of 12-week-old offspring rats of control group (P < 0.01). The protein expression of AngII and AngII type 1 receptor in thoracic aorta vessel were significantly higher while protein expression of AngII type 2 receptor was lower in 15-week-old offspring rats of LPS group than in control group, resulting in a significant increase in the ratio of AngII type 1 receptor/AngII type 2 receptor in the aorta at 15-week-old of offspring rats than in 15-week-old offspring rats of control group (P < 0.01).


CONCLUSIONS
Prenatal lipopolysaccharide exposure results vascular renin-angiotensin system dysfunction, which may play an important role on the pathogenesis of hypertension development in offspring rats.
Institute of Materia Medica, College of Pharmacy, Third Military Medical University, Chongqing 400038, China.
Xiao-yan
XY
He
You-cai
YC
Deng
Shu-hui
SH
Li
Yan-ling
YL
Wei
Li-yun
LY
Hao
Xing-xing
XX
Zhang
Xiao-hui
XH
Li
D000804:Angiotensin II / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D001794:Blood Pressure; D005260:Female; D006973:Hypertension / Q000209:etiology* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000009:adverse effects*; D018811:Maternal Exposure / Q000009:adverse effects*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D012084:Renin-Angiotensin System / Q000187:drug effects*
D008070:Lipopolysaccharides; D000804:Angiotensin II