Determination of protective role of selenium against aflatoxin B1-induced DNA damage.
Selenium is an essential mineral for a healthy life. Appropriate doses of it may undertake a protective role in the organism. In this study, the protective role of selenium (Se(4+)) against aflatoxin B1 (AFB1)-induced DNA damage was determined using random amplified polymorphic DNA on two plants including Vicia faba and Zea mays. It was observed that the concentrations of 0.1, 0.2 and 0.4 ppm of AFB1 have increased polymorphism value, total chlorophyll inhibition rate (IRc, %) and total protein IR (IRp, %). Unlike protein, chlorophyll contents and genomic template stability were decreased. With the addition of different concentrations (0.8 and 80 ppm) of Se(4+) to the treated samples with AFB1, the values return to normal. An 800-ppm concentration of Se(4+), on the contrary, could not inhibit the toxicity of AFB1 but caused an increase in toxicity level of AFB1/enhanced the toxicity level of AFB1. Results suggested that Se(4+) has an antagonistic effect against AFB1 toxicity and that the degree of antagonistic effect of Se(4+) against AFB1 was related to its concentration.
Department of Biology, Ataturk University, Turkey.
Guleray
G
Agar
Lokman
L
Alpsoy
Sedat
S
Bozari
Filiz Aygun
FA
Erturk
Nalan
N
Yildirim
D016604:Aflatoxin B1 / Q000633:toxicity*; D000704:Analysis of Variance; D002734:Chlorophyll / Q000378:metabolism; D004249:DNA Damage / Q000187:drug effects*; D018744:DNA, Plant / Q000737:chemistry / Q000187:drug effects; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D020011:Protective Agents / Q000494:pharmacology*; D019105:Random Amplified Polymorphic DNA Technique; D012643:Selenium / Q000494:pharmacology*; D031307:Vicia faba / Q000187:drug effects / Q000235:genetics; D003313:Zea mays / Q000187:drug effects / Q000235:genetics
D018744:DNA, Plant; D010940:Plant Proteins; D020011:Protective Agents; D002734:Chlorophyll; D016604:Aflatoxin B1; D012643:Selenium