Genotoxicity evaluation of fullerene C60 nanoparticles in a comet assay using lung cells of intratracheally instilled rats.
The genotoxicity of fullerene C(60) nanoparticles was evaluated in vivo with comet assays using the lung cells of rats given C(60) nanoparticles. The C(60) nanoparticles were intratracheally instilled as a single dose at 0.5 or 2.5mg/kg or repeated dose at 0.1 or 0.5mg/kg, once a week for 5 weeks, to male rats. The lungs were obtained 3 or 24h after a single instillation and 3h after repeated instillation. Inflammatory responses were observed in the lungs obtained 24h after a single instillation at 2.5mg/kg and repeated instillation at 0.5mg/kg. Histopathological examinations revealed that C(60) nanoparticles caused slight changes including hemorrhages in alveoli and the cellular infiltration of macrophages and neutrophils in alveoli. In comet assays using rat lung cells, no increase in % Tail DNA was found in any group given C(60) nanoparticles. These findings indicate that C(60) nanoparticles had no potential for DNA damage in comet assays using the lungs cells of rats given C(60) even at doses causing inflammation.
Research Institute of Science for Safety and Sustainability, National Institute of Advanced Industrial Science and Technology (AIST), 16-1 Onogawa, Tsukuba, Ibaraki 305-8569, Japan. ema-makoto@aist.go.jp
Makoto
M
Ema
Jin
J
Tanaka
Norihiro
N
Kobayashi
Masato
M
Naya
Shigehisa
S
Endoh
Junko
J
Maru
Masayo
M
Hosoi
Miho
M
Nagai
Madoka
M
Nakajima
Makoto
M
Hayashi
Junko
J
Nakanishi
D000818:Animals; D020552:Comet Assay / Q000379:methods* / Q000592:standards; D004249:DNA Damage / Q000187:drug effects / Q000502:physiology; D037741:Fullerenes / Q000008:administration & dosage / Q000633:toxicity*; D007278:Injections, Spinal; D008168:Lung / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D008297:Male; D009152:Mutagenicity Tests / Q000379:methods / Q000592:standards; D053758:Nanoparticles / Q000008:administration & dosage / Q000633:toxicity*; D010316:Particle Size; D051381:Rats; D017207:Rats, Sprague-Dawley
D037741:Fullerenes; C069837:fullerene C60