Modular gene over-expression strategies for Candida albicans.
Over-expression is a valid functional genomics approach to characterise genes of unknown function on a genome-wide scale. Strains are engineered to over-express a specific gene and the resulting gain-of-function phenotype assessed. Here, we describe the strategy we are adopting to synthesise a Candida albicans ORFeome collection and the options available to create over-expressing strains from this collection.
Département Génomes et Génétique, Institut Pasteur, Unité Biologie et Pathogénicité Fongiques, Paris, France.
Vitor
V
Cabral
Murielle
M
Chauvel
Arnaud
A
Firon
Mélanie
M
Legrand
Audrey
A
Nesseir
Sophie
S
Bachellier-Bassi
Yogesh
Y
Chaudhari
Carol A
CA
Munro
Christophe
C
d'Enfert
D002176:Candida albicans / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal / Q000235:genetics*; D016681:Genome, Fungal / Q000235:genetics; D055106:Genome-Wide Association Study; D023281:Genomics / Q000379:methods*; D016366:Open Reading Frames / Q000235:genetics