The effect of secretory factors of adipose-derived stem cells on human keratinocytes.
The beneficial effects of adipose-derived stem cell conditioned medium (ADSC-CM) on skin regeneration have been reported. Although the mechanism of how ADSC-CM promotes skin regeneration is unclear, ADSC-CM contained various growth factors and it is an excellent raw material for skin treatment. ADSC-CM produced in a hypoxia condition of ADSC-in other words, Advanced Adipose-Derived Stem cell Protein Extract (AAPE)-has great merits for skin regeneration. In this study, human primary keratinocytes (HKs), which play fundamental roles in skin tissue, was used to examine how AAPE affects HK. HK proliferation was significantly higher in the experimental group (1.22 μg/mL) than in the control group. DNA gene chip demonstrated that AAPE in keratinocytes (p < 0.05) notably affected expression of 290 identified transcripts, which were associated with cell proliferation, cycle and migration. More keratinocyte wound healing and migration was shown in the experimental group (1.22 μg/mL). AAPE treatment significantly stimulated stress fiber formation, which was linked to the RhoA-ROCK pathway. We identified 48 protein spots in 2-D gel analysis and selected proteins were divided into 64% collagen components and 30% non-collagen components as shown by the MALDI-TOF analysis. Antibody array results contained growth factor/cytokine such as HGF, FGF-1, G-CSF, GM-CSF, IL-6, VEGF, and TGF-β3 differing from that shown by 2-D analysis.


CONCLUSIONS
AAPE activates HK proliferation and migration. These results highlight the potential of the topical application of AAPE in the treatment of skin regeneration.
Department of Biomaterial Control, Dong-Eui University, Busan 614-714, Korea.
Kyoung Mi
KM
Moon
Prostemics Research Institute, Seoul 135-816, Korea.
Ye-Hyoung
YH
Park
Department of Biomaterial Control, Dong-Eui University, Busan 614-714, Korea.
Jae Seol
JS
Lee
Department of Chemistry, Dong-Eui University, Busan 614-714, Korea.
Yong-Byung
YB
Chae
Department of Chemistry, Dong-Eui University, Busan 614-714, Korea.
Moon-Moo
MM
Kim
Prostemics Research Institute, Seoul 135-816, Korea.
Dong-Soo
DS
Kim
Blue-Bio Regional Innovation Center, Dong-Eui University, Busan 614-714, Korea.
Byung-Woo
BW
Kim
Department of Biomaterial Control, Dong-Eui University, Busan 614-714, Korea.|Blue-Bio Regional Innovation Center, Dong-Eui University, Busan 614-714, Korea.|Department of Biotechnology and Bioengineering, Dong-Eui University, Busan 614-714, Korea.
Soo-Wan
SW
Nam
Department of Biomaterial Control, Dong-Eui University, Busan 614-714, Korea.|Blue-Bio Regional Innovation Center, Dong-Eui University, Busan 614-714, Korea.|Department of Biotechnology and Bioengineering, Dong-Eui University, Busan 614-714, Korea.
Jong-Hwan
JH
Lee
D000273:Adipose Tissue / Q000166:cytology*; D002465:Cell Movement; D017077:Culture Media, Conditioned / Q000032:analysis / Q000494:pharmacology; D016207:Cytokines / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D022502:Stress Fibers / Q000378:metabolism; D054460:rho-Associated Kinases / Q000378:metabolism; D020742:rhoA GTP-Binding Protein / Q000378:metabolism
D017077:Culture Media, Conditioned; D016207:Cytokines; D036341:Intercellular Signaling Peptides and Proteins; D054460:rho-Associated Kinases; D020742:rhoA GTP-Binding Protein