Effects of anti-CD3 monoclonal antibody in salivary glands of spontaneously diabetic mice.
BACKGROUND
Diabetes mellitus results in many complications, also compromising the salivary glands. The current treatment for this condition should be a substituting method to exogenous insulin. In this aspect, the immunotherapy has been tested, but, it can be inefficient as an agent for the control of damage caused by diabetes. Thus, the aim of this study was to evaluate the anti-CD3 monoclonal antibody as alternative immunotherapy in the recovery of salivary glands of spontaneously diabetic NOD (nonobese diabetic) mice.


METHODS
NOD mice were divided into two groups of 10 animals: group I (untreated diabetic mice) and group II (anti-CD3-treated diabetic mice). After treatment, the samples of salivary glands were collected for histological examination under both transmitted and polarized light microscopy.


RESULTS
Alterations in tissue architecture; increase in extracellular matrix and presence of inflammatory process were observed in untreated animals. Recovery of the salivary acinar cells occurred in treated animals. The parotid glands demonstrated a smaller amount of collagen fibers and were not observed severe inflammatory processes.


CONCLUSIONS
These results indicate that immunotherapy contributed to reestablishment of tissue damaged by the hyperglycemic condition, demonstrating that the immunomodulation plays an important role in the recovery of salivary glands.
Department of Morphology and Basic Pathology, Tissue Morphology Laboratory, Faculty of Medicine of Jundiaí, FMJ, Jundiaí, São Paulo, Brazil.
Hugo Tadeu
HT
Metidieri
Rafael Dias
RD
Mancio
Éber Emanuel
ÉE
Mayoral
Fernanda Alvarez
FA
Rojas
Luis Antonio
LA
Peroni
Alan Telles
AT
Ferri
Edmir Américo
EA
Lourenço
Eduardo José
EJ
Caldeira
D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage*; D017252:CD3 Complex / Q000276:immunology / Q000378:metabolism*; D048909:Diabetes Complications / Q000628:therapy*; D003922:Diabetes Mellitus, Type 1 / Q000150:complications*; D006651:Histocytochemistry; D007167:Immunotherapy / Q000379:methods*; D051379:Mice; D016688:Mice, Inbred NOD; D008853:Microscopy; D012469:Salivary Glands / Q000473:pathology*
D000911:Antibodies, Monoclonal; D017252:CD3 Complex