[Excretion of extracellular membrane nanovesicles by aeromonas].
OBJECTIVE
Study of extracellular membrane nanovesicles production by Aeromonas hydrophila and Aeromonas salmonicida bacteria on a subcellular level.


METHODS
4 strains of A. hydrophila: 342-1, E 8-8, H 336 and H 1-6-05 and 1 strain of A. salmonicida A-450 as well as intact Wistar line rats were used. Methods of transmission electron microscopy: ultrathin sectioning and negative contrasting were used. RESULTS. A. hydrophila and A. salmonicida bacteria produced in pure cultures excrete into the environment extracellular membrane nanovesicles. The size of these vesicles varies from 20 to 200 nm in diameter. The process of gemmation from bacterial cell and possibility of obtaining isolated membrane nanovesicles preparations is shown. These vesicles are detected in ultrathin sections of apical surface of intact rat intestine among accumulations ofparietal microorganisms that colonize mucous membrane. Extracellular membrane nanovesicles excreted by aeromonas are analogous by size and ultrastructure to vesicles of other species of gram-negative bacteria described in the literature.


CONCLUSIONS
During production of A. hydrophila and A. salmonicida bacteria in vitro nanovesicles are formed from the outer membranes of the cells and excreted into the environment, the nanovesicles are similar to those detected in ultrathin sections of the surface of intestine of rats among accumulations of parietal microorganisms and in glycocalix between epitheliocyte microvilli.
K A
KA
Lusta
Iu E
IuE
Kozlovskiĭ
D016980:Aeromonas hydrophila / Q000502:physiology* / Q000648:ultrastructure; D048409:Aeromonas salmonicida / Q000502:physiology* / Q000648:ultrastructure; D000818:Animals; D001692:Biological Transport / Q000502:physiology; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure*; D007413:Intestinal Mucosa / Q000382:microbiology*; D008854:Microscopy, Electron; D008867:Microtomy; D008871:Microvilli / Q000382:microbiology; D053141:Organelle Size; D015388:Organelles / Q000378:metabolism / Q000648:ultrastructure*; D053038:Quorum Sensing / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D013194:Staining and Labeling