Activity, polypeptide and gene identification of thylakoid Ndh complex in trees: potential physiological relevance of fluorescence assays.
Three evergreen (Laurus nobilis, Viburnum tinus and Thuja plicata) and two autumnal abscission deciduous trees (Cydonia oblonga and Prunus domestica) have been investigated for the presence (zymogram and immunodetection) and functionality (post-illumination chlorophyll fluorescence) of the thylakoid Ndh complex. The presence of encoding ndh genes has also been investigated in T. plicata. Western assays allowed tentative identification of zymogram NADH dehydrogenase bands corresponding to the Ndh complex after native electrophoresis of solubilized fractions from L. nobilis, V. tinus, C. oblonga and P. domestica leaves, but not in those of T. plicata. However, Ndh subunits were detected after SDS-PAGE of thylakoid solubilized proteins of T. plicata. The leaves of the five plants showed the post-illumination chlorophyll fluorescence increase dependent on the presence of active Ndh complex. The fluorescence increase was higher in autumn in deciduous, but not in evergreen trees, which suggests that the thylakoid Ndh complex could be involved in autumnal leaf senescence. Two ndhB genes were sequenced from T. plicata that differ at the 350 bp 3' end sequence. Comparison with the mRNA revealed that ndhB genes have a 707-bp type II intron between exons 1 (723 bp) and 2 (729 bp) and that the UCA 259th codon is edited to UUA in mRNA. Phylogenetically, the ndhB genes of T. plicata group close to those of Metasequoia, Cryptomeria, Taxodium, Juniperus and Widdringtonia in the cupresaceae branch and are 5' end shortened by 18 codons with respect to that of angiosperms.
Departamento de Biología Vegetal, Universidad de Alcalá, Alcalá de Henares, 28871 Madrid, Spain.
Patricia H
PH
Serrot
Bartolomé
B
Sabater
Mercedes
M
Martín
D000375:Aging / Q000502:physiology; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005453:Fluorescence; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D027442:Laurus / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D018515:Plant Leaves / Q000737:chemistry*; D027861:Prunus / Q000235:genetics / Q000378:metabolism; D027824:Rosaceae / Q000235:genetics / Q000378:metabolism; D012621:Seasons; D029783:Thuja / Q000235:genetics / Q000378:metabolism; D020524:Thylakoids / Q000235:genetics* / Q000378:metabolism*; D014197:Trees / Q000235:genetics* / Q000378:metabolism*; D027925:Viburnum / Q000235:genetics / Q000378:metabolism
D009245:NADH Dehydrogenase