[Preparation and partial characterization of monoclonal antibodies against HA protein of H1 subtype influenza virus].
OBJECTIVE
Preparation of H1 subtype of influenza virus HA protein monoclonal antibody (mAbs),  and to analyse their reactivity.


METHODS
H1N1 influenza virus vaccine (2009) and seasonal A1 influenza virus vaccine as antigen,  conventional immunization,  fusion,  cloning,  access to the antigen-specific mAbs. To study the reactivity and specificity of mAbs by ELISA,  HI test and Western blot.


RESULTS
We have obtained 97 hybridoma of stable secreting anti-H1 subtype of influenza virus HA protein. According to their different reactivity,  these mAbs can be divided into four categories:  39 strains for strain-specific,  of which 29 have HI activity;  7 strains for subtype-specific,  of which 5 have HI activity;  16 strains for the 2009 pandemic strain and the seasonal strains to common antigens,  of which 9 have HI activity;  35 strains common for influenza virus antigens,  of which 22 have HI activity.


CONCLUSIONS
Both vaccines have better immunogenicity and immune protection activity. Preparation of four types antibody against HA protein of H1 subtype of influenza virus,  can provide experimental data for preparation subtype-specific diagnostic kits and influenza A and B virus diagnostic kit,  and lay a foundation for further studying of H1N1 influenza virus HA epitope.
Xi'an Jiaotong University College of Medicine, Xi'an, China.
Chun-yan
CY
Guo
Hui-jin
HJ
Li
Yang
Y
Liu
Xiang-rong
XR
Zhao
Xin
X
Wang
Qing
Q
Feng
Yuan
Y
Li
Jun
J
Hu
D000818:Animals; D000911:Antibodies, Monoclonal / Q000145:classification / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology; D003429:Cross Reactions / Q000276:immunology; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000276:immunology*; D006801:Humans; D006825:Hybridomas; D007132:Immunoglobulin Isotypes / Q000276:immunology; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology*; D051379:Mice
D000911:Antibodies, Monoclonal; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007132:Immunoglobulin Isotypes