Wnt/β-catenin signaling changes C2C12 myoblast proliferation and differentiation by inducing Id3 expression.
Canonical Wnt signaling plays important roles in regulating cell proliferation and differentiation. In this study, we report that inhibitor of differentiation (Id)3 is a Wnt-inducible gene in mouse C2C12 myoblasts. Wnt3a induced Id3 expression in a β-catenin-dependent manner. Bone morphogenetic protein (BMP) also potently induced Id3 expression. However, Wnt-induced Id3 expression occurred independent of the BMP/Smad pathway. Functional studies showed that Id3 depletion in C2C12 cells impaired Wnt3a-induced cell proliferation and alkaline phosphatase activity, an early marker of osteoblast cells. Id3 depletion elevated myogenin induction during myogenic differentiation and partially impaired Wnt3a suppressed myogenin expression in C2C12 cells. These results suggest that Id3 is an important Wnt/β-catenin induced gene in myoblast cell fate determination.
Dept. of Molecular Cell Biology and Centre for Biomedical Genetics, Leiden University Medical Center, Leiden, The Netherlands.
Long
L
Zhang
Songting
S
Shi
Juan
J
Zhang
Fangfang
F
Zhou
Peter
P
ten Dijke
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics*; D002460:Cell Line; D049109:Cell Proliferation; D051796:Inhibitor of Differentiation Proteins / Q000235:genetics*; D051379:Mice; D032446:Myoblasts / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D041681:NIH 3T3 Cells; D015398:Signal Transduction; D051785:Smad Proteins / Q000378:metabolism; D060509:Wnt3A Protein / Q000235:genetics / Q000378:metabolism*; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism*
D019485:Bone Morphogenetic Proteins; C495267:CTNNB1 protein, mouse; D051796:Inhibitor of Differentiation Proteins; D051785:Smad Proteins; D060509:Wnt3A Protein; C555511:Wnt3a protein, mouse; D051176:beta Catenin; C067679:Idb3 protein, mouse; D000469:Alkaline Phosphatase