Development of a reinforced Ti-eviction plasmid useful for construction of Ti plasmid-free Agrobacterium strains.
For curing stable Ti plasmids from Agrobacterium strains, we developed a new Ti-eviction plasmid. The plasmid contains two antitoxin genes, which originated from two toxin-antitoxin systems in Ti plasmids. The plasmid facilitated production of Ti-less cells in seven tested strains, and was removed easily by simple counter-selection from the cells.
Department of Biological Science, Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Hiroshima 739–8526, Japan. syamamo@hiroshima-u.ac.jp
Shinji
S
Yamamoto
Katsunori
K
Suzuki
D060054:Agrobacterium / Q000235:genetics*; D001427:Bacterial Toxins; D042822:Genomic Instability; D050296:Microbial Viability; D036741:Plant Tumor-Inducing Plasmids; D012641:Selection, Genetic; D017384:Sequence Deletion
D001427:Bacterial Toxins