Isolation and characterization of zygomycetes fungi from tempe for ethanol production and biomass applications.
Mixed fungal cultures used for making tempe, a fermented soy bean food, were screened for biomass conversion. Thirty-two zygomycetes strains from two tempe cultures were isolated and identified as Rhizopus, Mucor, Rhizomucor, and Absidia species based upon morphology. The dry weight biomass of these strains contained 49% to 63% protein and 10-24% chitosan. The strains with the best growth performance were selected and registered at Culture Collection of Gothenburg University as Rhizomucor CCUG 61146 and Rhizomucor CCUG 61147. These strains were able to grow both aerobically and micro-aerobically. Their ethanol yields were 0.38-0.47, 0.19-0.22, and 0.31-0.38 g/g on glucose, xylose, and a mix sugars consisting of cellobiose, glucose, xylose, arabinose, galactose, and mannose, respectively. The biomass yield of the strains varied between 65 and 140 mg dry weight/g glucose.
School of Engineering, University of Borås, Borås, Sweden.
Rachma
R
Wikandari
Ria
R
Millati
Patrik R
PR
Lennartsson
Eni
E
Harmayani
Mohammad J
MJ
Taherzadeh
D018533:Biomass; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol / Q000378:metabolism*; D009090:Mucorales / Q000302:isolation & purification* / Q000378:metabolism; D045730:Soy Foods / Q000382:microbiology*; D017550:Spectroscopy, Fourier Transform Infrared; D013172:Spores, Fungal
D000431:Ethanol