Reveal E. coli 2.0 method for detection of Escherichia coli O157:H7 in raw beef.
Reveal E. coli 2.0 is a new lateral-flow immunodiagnostic test for detection of E. coli O157:H7 and O157:NM in raw beef trim and ground beef. Compared with the original Reveal E. coli O157:H7 assay, the new test utilizes a unique antibody combination resulting in improved test specificity. The device architecture and test procedure have also been modified, and a single enrichment protocol was developed which allows the test to be performed at any point during an enrichment period of 12 to 20 h. Results of inclusivity and exclusivity testing showed that the test is specific for E. coli serotypes O157:H7 and O157:NM, with the exception of two strains of O157:H38 and one strain of O157:H43 which produced positive reactions. In internal and independent laboratory trials comparing the Reveal 2.0 method to the U.S. Department of Agriculture-Food Safety and Inspection Service reference culture procedure for detection of E. coli O157:H7 in 65 and 375 g raw beef trim and ground beef samples, there were no statistically significant differences in method performance with the exception of a single internal trial with 375 g ground beef samples in which the Reveal method produced significantly more positive results. There were no unconfirmed positive results by the Reveal assay, for specificity of 100%. Results of ruggedness testing showed that the Reveal test produces accurate results even with substantial deviation in sample volume or device incubation time or temperature. However, addition of the promoter reagent to the test sample prior to introducing the test device is essential to proper test performance.
Neogen Corp., 620 Lesher Pl, Lansing, MI 48912, USA.
Rebecca
R
Hoerner
Jill
J
Feldpausch
R Lucas
RL
Gray
Stephanie
S
Curry
Paul
P
Lewis
Jerry
J
Tolan
Tim
T
Goldy
Frank
F
Klein
Barry
B
Neiditch
Edan
E
Hosking
Paul
P
Norton
Jennifer
J
Rice
Mark
M
Mozola
D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods; D002417:Cattle; D004269:DNA, Bacterial / Q000032:analysis; D019453:Escherichia coli O157 / Q000302:isolation & purification*; D005506:Food Contamination; D005516:Food Microbiology / Q000379:methods; D007118:Immunoassay / Q000379:methods*; D008461:Meat Products / Q000382:microbiology*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D014481:United States; D016885:United States Department of Agriculture
D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic