Production of the monoclonal antibody against Sudan 2 for immunoassay of Sudan dyes in egg.
Many methods have been reported to determine the residues of Sudan dyes in food samples. Among the reported methods, enzyme-linked immunosorbent assay (ELISA) was a frequently used practical screen tool. In this study, a novel hapten of Sudan 2 was synthesized by coupling 4-amino-3-methylbenzoic acid to β-naphthol, and the monoclonal antibody against Sudan 2 was produced. The obtained antibody can recognize Sudan 1, 2, 3, and 4, Sudan red G, and Para red simultaneously. After evaluation of different coating antigens, a heterologous indirect competitive ELISA was then developed to determine the six Sudan dyes in egg. The cross-reactivities for the six analytes were in a range of 63% to 100%, and the limits of detection were in a range of 0.2 to 0.5 ng/g, depending on the compound. Intra- and interassay recoveries from the standard fortified blank eggs were in a range of 71.7% to 97.6% with coefficients of variation lower than 17.1%.
College of Animal Science and Technology, Agricultural University of Hebei, Baoding, Hebei 071000, China.
Jing
J
Liu
Huicai
H
Zhang
Dongsheng
D
Zhang
Feng
F
Gao
Jianping
J
Wang
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism*; D001391:Azo Compounds / Q000032:analysis* / Q000276:immunology; D004531:Eggs / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D006241:Haptens / Q000276:immunology
D000911:Antibodies, Monoclonal; D001391:Azo Compounds; D006241:Haptens; C033003:sudan red