Hydroa vacciniforme is associated with increased numbers of Epstein-Barr virus-infected γδT cells.
Hydroa vacciniforme (HV) is a rare photosensitivity disorder of childhood associated with Epstein-Barr virus (EBV)(+) T-cell infiltration. We have summarized clinical manifestations of HV, and analyzed EBV(+) T-cell subsets as well as EBV DNA load in lymphocyte fractions, in comparison with hypersensitivity to mosquito bites (HMB), an EBV-associated T/natural killer (NK) lymphoproliferative disorder. We found that 31 of 33 (93.9%) HV lesions were composed of EBV(+) T cells and reactive EBV(-) cytotoxic T cells, without significant CD56(+) cell infiltration, whereas many CD56(+) cells were present in 8 of 9 (88.9%) HMB lesions. Of 13 (20.6%) HMB patients with or without HV, 12 (92.3%) showed increased percentages (>32%) of NK cells in the peripheral blood, whereas in the 16 patients with HV alone, 14 (87.5%) showed no increase. Of the 11 HV patients, 10 (90.9%) had increased percentages (>5%) of circulating γδT cells, with a mean value of 15.7 ± 2.9%, and the γδT-cell fractions contained larger amounts of EBV DNA than non-γδT-cell fractions. A triple-labeling method revealed that all three HV patients examined had increased percentages of EBER(+), T-cell receptor (TCR)γδ(+), and TCRαβ(-) cells. Our observations indicate that HV is associated with increased numbers of EBV(+) γδT cells, whereas HMB is associated with EBV(+) NK cells.
Department of Dermatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Kawasaki Medical School, Okayama, Japan.
Yoji
Y
Hirai
Takenobu
T
Yamamoto
Hiroshi
H
Kimura
Yoshinori
Y
Ito
Kazuhide
K
Tsuji
Tomoko
T
Miyake
Shin
S
Morizane
Daisuke
D
Suzuki
Kazuyasu
K
Fujii
Keiji
K
Iwatsuki
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D019002:CD56 Antigen / Q000032:analysis; D002648:Child; D002675:Child, Preschool; D009033:Culicidae; D004279:DNA, Viral / Q000097:blood; D020031:Epstein-Barr Virus Infections / Q000150:complications / Q000276:immunology; D005260:Female; D004854:Herpesvirus 4, Human; D006801:Humans; D006837:Hydroa Vacciniforme / Q000150:complications / Q000276:immunology / Q000821:virology*; D007299:Insect Bites and Stings / Q000276:immunology*; D007694:Killer Cells, Natural; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D012367:RNA, Viral / Q000032:analysis; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000378:metabolism*; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000821:virology*; D013602:T-Lymphocytes, Cytotoxic; D019562:Viral Load; D055815:Young Adult
D019002:CD56 Antigen; D004279:DNA, Viral; D012367:RNA, Viral; D016692:Receptors, Antigen, T-Cell, gamma-delta