Polymerase chain reaction assay for rapid, sensitive detection, and identification of Colletotrichum gloeosporioides causing greater yam anthracnose.
Anthracnose caused by Colletotrichum gloeosporioides is an economically important disease which affects greater yam (Dioscorea alata L.) worldwide. Apart from airborne conidia, the pathogen propagules surviving in soil and planting material are the major sources of inoculum. A nested PCR assay has been developed for specific detection of C. gloeosporioides in soil and planting material. In conventional (single-round) PCR, the limit of detection was 20 pg, whereas in nested PCR the detection limit increased to 0.2 pg of DNA. The primers designed were found to be highly specific and could be used for accurate identification of the pathogen up to species level. The protocol was standardized for detection of the pathogen in artificially and naturally infected field samples.
Division of Crop Protection, Central Tuber Crops Research Institute, Sreekariyam, Thiruvananthapuram, 695017 Kerala, India.
Mithun
M
Raj
M  L
M
Jeeva
V
V
Hegde
Pravi
P
Vidyadharan
P  V
P
Archana
M
M
Senthil alias Sankar
S Vishnu
SV
Nath
D020231:Colletotrichum / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D027742:Dioscorea / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013045:Species Specificity; D013172:Spores, Fungal / Q000235:genetics / Q000302:isolation & purification
D017931:DNA Primers; D004271:DNA, Fungal