Stochastic mapping of the Michaelis-Menten mechanism.
The Michaelis-Menten mechanism is an extremely important tool for understanding enzyme-catalyzed transformation of substrates into final products. In this work, a computationally viable, full stochastic description of the Michaelis-Menten kinetic scheme is introduced based on a stochastic equivalent of the steady-state assumption. The full solution derived is free of restrictions on amounts of substance or parameter values and is used to create stochastic maps of the Michaelis-Menten mechanism, which show the regions in the parameter space of the scheme where the use of the stochastic kinetic approach is inevitable. The stochastic aspects of recently published examples of single-enzyme kinetic studies are analyzed using these maps.
Department of Inorganic and Analytical Chemistry, University of Debrecen, Debrecen, Hungary.
Éva
É
Dóka
Gábor
G
Lente
D000465:Algorithms; D002384:Catalysis; D004798:Enzymes; D007700:Kinetics; D008954:Models, Biological
D004798:Enzymes