Lansoprazole induces collagenous colitis in the colon of Mongolian gerbils.
Collagenous colitis (CC) is an illness characterized by chronic diarrhea with possible effects on neoplastic development, but there have been no reports in animals. We therefore tried to establish CC development in a Mongolian gerbil (MG) model by long-term continuous lansoprazole (LPZ) administration and aimed to clarify the relationship between LPZ administration and CC occurrence. We divided 69 gerbils into 6 groups: Helicobacter pylori (Hp)-infected+high-dose LPZ, Hp-infected+low-dose-LPZ, Hp-infected, high-dose-LPZ, low- dose-LPZ, and control. The gerbils were sacrificed and entire colons were excised at experimental weeks 27, 54, and 108. We examined colonic lesions by staining of Swiss-roll intestines pathologically. A total of 3 gerbils had CC-like lesions in the proximal colon. All MGs with CC-like lesions were from LPZ treated groups (3 of 35; 8.6%). The thickened subepithelial collagen band detected in these lesions strongly resembled that of human CC lesions. Immunohistochemical analysis indicated a tendency for more chromogranin A-positive cells in the upper layer of colonic crypt following continuous LPZ administration. In conclusion, we successfully established development of CC-like lesions in an MG model by continuous LPZ administration and determined that the ectopic endocrine cells that were induced by LPZ administration may influence the occurrence of these lesions in the colon.
Department of Gastroenterology and Metabolism, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan.
Takashi
T
Mizushima
Tsutomu
T
Mizoshita
Makoto
M
Sasaki
Satoshi
S
Tanida
Hironobu
H
Tsukamoto
Takaya
T
Shimura
Takayoshi
T
Kanematsu
Hiromi
H
Kataoka
Takeshi
T
Kamiya
Tetsuya
T
Tsukamoto
Masae
M
Tatematsu
Takashi
T
Joh
D053799:2-Pyridinylmethylsulfinylbenzimidazoles / Q000008:administration & dosage / Q000009:adverse effects* / Q000494:pharmacology; D000818:Animals; D046729:Colitis, Collagenous / Q000139:chemically induced* / Q000473:pathology*; D003106:Colon / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D005753:Gastric Mucosa / Q000473:pathology; D005849:Gerbillinae; D016481:Helicobacter Infections / Q000473:pathology; D016480:Helicobacter pylori; D064747:Lansoprazole
D053799:2-Pyridinylmethylsulfinylbenzimidazoles; D064747:Lansoprazole