Curcumin causes promoter hypomethylation and increased  expression of FANCF gene in SiHa cell line.
Curcumin and resveratrol were evaluated for their potential to cause reversal of promoter hypermethylation and associated gene expression of FANCF in SiHa cell line. Methylation specific PCR along with bisulphite sequencing revealed the demethylation of 12 CpG sites out of 15 CpG sites spanning ?280 to ?432 region of FANCF promoter after treatment with curcumin and fivefold up regulation of FANCF gene expression as shown by qRT-PCR. In vitro methylation assay also showed that M.SssI an analogue of DNMT1 was effectively inhibited at 50 lM concentration of curcumin. Resveratrol was not found to be effective in causing reversal of promoter hypermethylation of FANCF gene when used at 20 lM for 4 days in SiHa cell line.
Department of Biotechnology, Panjab University, Sector 14, Chandigarh 160014, India. parashar_panchal@yahoo.co.in
Gaurav
G
Parashar
Nidarshana Chaturvedi
NC
Parashar
Neena
N
Capalash
D001374:Azacitidine / Q000031:analogs & derivatives / Q000494:pharmacology; D001483:Base Sequence; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003474:Curcumin / Q000494:pharmacology*; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000037:antagonists & inhibitors / Q000737:chemistry; D019175:DNA Methylation; D018076:DNA, Complementary / Q000737:chemistry; D015257:DNA-Cytosine Methylases / Q000737:chemistry; D000077209:Decitabine; D044127:Epigenesis, Genetic; D052219:Fanconi Anemia Complementation Group F Protein / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D020128:Inhibitory Concentration 50; D011401:Promoter Regions, Genetic; D000077185:Resveratrol; D017422:Sequence Analysis, DNA; D013267:Stilbenes / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects*
D018076:DNA, Complementary; C499901:FANCF protein, human; D052219:Fanconi Anemia Complementation Group F Protein; D013267:Stilbenes; D000077209:Decitabine; C081573:DNA modification methylase SssI; D015257:DNA-Cytosine Methylases; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases; C000619380:DNMT1 protein, human; D003474:Curcumin; D001374:Azacitidine; D000077185:Resveratrol