Synthesis, characterization, and cytotoxicity in vitro of the complex [Mn (Hptc) (phen) (OH)] n.
OBJECTIVE
A bridging ligand 2,4,6-pyridine tricarboxylic acid (H(3)ptc) and its manganese(II) complex [Mn(Hptc)(phen)(OH)]n(Hptc=2,4,6-pyridine tricarboxylic acid, phen=1,10-phenanthroline) have been synthesized and characterized.


METHODS
The interaction with DNA (HeLa and KB) was carried out by fluorescence spectrum and gel electrophoresis assay. In vitro apoptosis assay and cytotoxicity assay detect the manganese (II) complex interaction with cancer cells.


RESULTS
Fluorescence spectrum demonstrated the ability of the complexes to interact with DNA in an intercalative mode. Gel electrophoresis assay exhibited more effective DNA-cleavage activity. In vitro apoptosis assay of the complexes were examined on HeLa and KB cells, exhibited cytotoxic specificity and a significant cancer cell inhibitory rate.


CONCLUSIONS
The complex may be a latent antitumor agent as a result of its unique interaction mode with DNA and cancer cells inhibition effect.
Department of Coordination Chemistry, Shenyang University of Chemical Technology, Shenyang 110142, China.
MingChang
M
Zhu
WenXuan
W
He
EnJun
E
Gao
Lin
L
Lin
Ying
Y
Zhang
Lei
L
Dai
RenShu
R
Wang
Bo
B
Wang
MeiLin
M
Wang
D017209:Apoptosis / Q000187:drug effects; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D007624:KB Cells; D008024:Ligands; D017895:Manganese Compounds / Q000138:chemical synthesis* / Q000737:chemistry*; D008958:Models, Molecular; D010618:Phenanthrolines / Q000138:chemical synthesis* / Q000737:chemistry* / Q000633:toxicity*; D013050:Spectrometry, Fluorescence
D008024:Ligands; D017895:Manganese Compounds; D010618:Phenanthrolines; D004247:DNA