Spatiotemporal control of gene expression by a light-switchable transgene system.
We developed a light-switchable transgene system based on a synthetic, genetically encoded light-switchable transactivator. The transactivator binds promoters upon blue-light exposure and rapidly initiates transcription of target transgenes in mammalian cells and in mice. This transgene system provides a robust and convenient way to spatiotemporally control gene expression and can be used to manipulate many biological processes in living systems with minimal perturbation.
Synthetic Biology and Biotechnology Laboratory, State Key Laboratory of Bioreactor Engineering, School of Pharmacy, East China University of Science and Technology, Shanghai, China.
Xue
X
Wang
Xianjun
X
Chen
Yi
Y
Yang
D004247:DNA / Q000235:genetics* / Q000528:radiation effects*; D015870:Gene Expression / Q000235:genetics* / Q000528:radiation effects*; D008027:Light; D011829:Radiation Dosage; D019076:Transgenes / Q000235:genetics* / Q000528:radiation effects*
D004247:DNA