Protein profiling in potato (Solanum tuberosum L.) leaf tissues by differential centrifugation.
Foliar diseases, such as late blight, result in serious threats to potato production. As such, potato leaf tissue becomes an important substrate to study biological processes, such as plant defense responses to infection. Nonetheless, the potato leaf proteome remains poorly characterized. Here, we report protein profiling of potato leaf tissues using a modified differential centrifugation approach to separate the leaf tissues into cell wall and cytoplasmic fractions. This method helps to increase the number of identified proteins, including targeted putative cell wall proteins. The method allowed for the identification of 1484 nonredundant potato leaf proteins, of which 364 and 447 were reproducibly identified proteins in the cell wall and cytoplasmic fractions, respectively. Reproducibly identified proteins corresponded to over 70% of proteins identified in each replicate. A diverse range of proteins was identified based on their theoretical pI values, molecular masses, functional classification, and biological processes. Such a protein extraction method is effective for the establishment of a highly qualified proteome profile.
Department of Plant and Animal Sciences, Nova Scotia Agricultural College, Truro, Nova Scotia, Canada.
Sanghyun
S
Lim
Kenneth
K
Chisholm
Robert H
RH
Coffin
Rick D
RD
Peters
Khalil I
KI
Al-Mughrabi
Gefu
G
Wang-Pruski
Devanand M
DM
Pinto
D002473:Cell Wall / Q000737:chemistry; D002498:Centrifugation / Q000379:methods*; D003593:Cytoplasm / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D010940:Plant Proteins / Q000032:analysis* / Q000737:chemistry; D020543:Proteome / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics / Q000379:methods; D015203:Reproducibility of Results; D011198:Solanum tuberosum / Q000737:chemistry* / Q000378:metabolism
D010940:Plant Proteins; D020543:Proteome