Analysis of expressed sequence tags from grapevine flower and fruit and development of simple sequence repeat markers.
A total of 6,230 EST sequences were produced from 7,561 clones in a cDNA library generated from grapevine (Vitis vinifera cv. 'Summer Black') flower and fruit tissues in this study. After cluster and assembly analysis of the datasets, 3,582 unigenes (GenBank accession numbers GW836604-GW840185) were established, among which 381 were new grapevine EST sequences. Out of the 381 new ESTs, 289 could be mapped on the 19 grapevine chromosomes. 913 unique ESTs with known or putative functions were assigned to 11 putative cellular roles. 540 potentially workable grapevine EST-SSRs were developed from 3,582 unigenes and about 42.6% of these unigenes were identified as true-to-type SSR loci and could amplify polymorphic bands from 22 individual plants of V. vinifera L, indicating that grapevine EST datasets are a valuable source for the development of functional simple sequence repeat (SSR) markers.
College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu, People's Republic of China.
Xi-Cheng
XC
Wang
Lei
L
Guo
Ling-Fei
LF
Shangguan
Chen
C
Wang
Guang
G
Yang
Shen-Chun
SC
Qu
Jing-Gui
JG
Fang
D001483:Base Sequence; D002874:Chromosome Mapping; D016000:Cluster Analysis; D057225:Data Mining; D020224:Expressed Sequence Tags; D035264:Flowers / Q000235:genetics*; D005638:Fruit / Q000235:genetics*; D015723:Gene Library; D017343:Genes, Plant; D005819:Genetic Markers; D018895:Microsatellite Repeats; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D027843:Vitis / Q000235:genetics*
D005819:Genetic Markers