Efficient one-pot multienzyme synthesis of UDP-sugars using a promiscuous UDP-sugar pyrophosphorylase from Bifidobacterium longum (BLUSP).
A promiscuous UDP-sugar pyrophosphorylase (BLUSP) was cloned from Bifidobacterium longum strain ATCC55813 and used efficiently with a Pasteurella multocida inorganic pyrophosphatase (PmPpA) with or without a monosaccharide 1-kinase for one-pot multienzyme synthesis of UDP-galactose, UDP-glucose, UDP-mannose, and their derivatives. Further chemical diversification of a UDP-mannose derivative resulted in the formation of UDP-N-acetylmannosamine.
Department of Chemistry, University of California, One Shields Avenue, Davis, California 95616, USA.
Musleh M
MM
Muthana
Jingyao
J
Qu
Yanhong
Y
Li
Lei
L
Zhang
Hai
H
Yu
Li
L
Ding
Hamed
H
Malekan
Xi
X
Chen
D001644:Bifidobacterium / Q000201:enzymology*; D005686:Galactokinase / Q000378:metabolism; D043564:Inorganic Pyrophosphatase / Q000378:metabolism; D016979:Pasteurella multocida / Q000201:enzymology; D005957:UTP-Glucose-1-Phosphate Uridylyltransferase / Q000378:metabolism*; D014531:Uridine Diphosphate Galactose / Q000096:biosynthesis / Q000737:chemistry; D014532:Uridine Diphosphate Glucose / Q000096:biosynthesis / Q000737:chemistry; D014539:Uridine Diphosphate Sugars / Q000096:biosynthesis* / Q000737:chemistry
D014539:Uridine Diphosphate Sugars; C042056:uridine diphosphate mannose; D014531:Uridine Diphosphate Galactose; D005686:Galactokinase; D005957:UTP-Glucose-1-Phosphate Uridylyltransferase; D043564:Inorganic Pyrophosphatase; D014532:Uridine Diphosphate Glucose