Simultaneous determination of five polyether ionophores using liquid chromatography with one-step fluorescent derivatization.
We present a selective method for simultaneous determination of five polyether ionophores such as salinomycin (SAL), monensin (MON), narasin (NAR), semduramicin (SEM) and lasalocid (LAS) in aquatic samples using a liquid chromatography with one-step fluorescent derivatization of 2-(4-hydrazinocarbonyl-phenyl) 4,5-diphenylimidazole (HCPI) and 4-(4,5-diphenyl-1H-imidazol-2-yl) benzoyl chloride hydrochloride (DIB-Cl). Fluorescent one-step derivatization for SAL, MON, NAR and SEM using HCPI and for LAS using DIB-Cl was monitored by an LC/fluorescence detector (E(x), 340 nm; E(m), 465 nm). Chromatographic separation was performed on a TSK-GEL ODS-120T (4.6 × 150 mm, 3 µm) column using a mobile phase of 0.1% formic acid in acetonitrile and 0.5 mM ammonium formate in water (70/30, v/v). The limits of detections were 0.01 µg/mL (50 pg) for LAS, 0.05 µg/mL (250 pg) for SAL, NAR and SEM, and 0.1 µg/mL (500 pg) for MON, respectively. The recoveries for water samples were indicated to be the range of 79.6 ± 6.4 - 99.0 ± 4.4% with associated precision values (between-day for 3 days) for repeatability. Based on solid-phase extraction, the limit of quantitation values indicated 0.1 ng/mL for SAL, MON, NAR and SEM, and 0.01 ng/mL for LAS in water samples.
Department of Physical and Analytical Chemistry, School of Pharmacy, Kinjo Gakuin University, 2-1723 Omori, Moriyama, Nagoya 463–8521, Japan. kinoue@kinjo-u.ac.jp
Koichi
K
Inoue
Yukiko
Y
Miura
Misato
M
Suzuki
Naoya
N
Kishikawa
Tomoaki
T
Hino
Naotaka
N
Kuroda
Hisao
H
Oka
D001565:Benzoates / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D004987:Ethers / Q000032:analysis* / Q000737:chemistry* / Q000302:isolation & purification; D005456:Fluorescent Dyes / Q000737:chemistry*; D007093:Imidazoles / Q000737:chemistry; D007476:Ionophores / Q000032:analysis* / Q000737:chemistry* / Q000302:isolation & purification; D052616:Solid Phase Extraction; D013050:Spectrometry, Fluorescence; D013997:Time Factors; D014867:Water / Q000737:chemistry
C114684:4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride; D001565:Benzoates; D004987:Ethers; D005456:Fluorescent Dyes; D007093:Imidazoles; D007476:Ionophores; D014867:Water