A filter-based propidium monoazide technique to distinguish live from membrane-compromised microorganisms using quantitative PCR.
Propidium monoazide (PMA) was used to differentiate live from membrane-compromised bacteria in PCR methods. We have adapted this technique for use on membrane-filtered water samples and determined its efficacy using qPCR. Independent labs at three institutions replicated these findings.
Center for Environmental Diagnostics and Bioremediation, University of West Florida, 11000 University Parkway, Pensacola, FL 32514, USA.
Kristen N
KN
Hellein
Elizabeth M
EM
Kennedy
Valerie J
VJ
Harwood
Katrina V
KV
Gordon
Shiao Y
SY
Wang
Joe Eugene
JE
Lepo
D001386:Azides / Q000378:metabolism*; D001431:Bacteriological Techniques / Q000379:methods*; D002462:Cell Membrane / Q000502:physiology*; D004791:Enzyme Inhibitors / Q000378:metabolism*; D005374:Filtration / Q000379:methods; D050296:Microbial Viability; D011419:Propidium / Q000031:analogs & derivatives* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D014871:Water Microbiology
D001386:Azides; D004791:Enzyme Inhibitors; C533957:propidium monoazide; D011419:Propidium