Effects of the synthetic coprisin analog peptide, CopA3 in pathogenic microorganisms and mammalian cancer cells.
A synthetic coprisin analog peptide, 9-mer dimer CopA3 (CopA3) was designed based on a defensin-like peptide, Coprisin, isolated from the bacteria-immunized dung beetle Copris tripartitus. Here, CopA3 was investigated for its antimicrobial activity and cancer cell growth inhibition. CopA3 showed antimicrobial activities against various pathogenic bacteria and yeast fungus with MIC values in 2~32 μM ranges, and inhibited the cell viabilities of pancreatic and hepatocellular cancer cells, except MIAPaca2, Hep3B, and HepG2 cells, in a dose-dependent manner. The average IC(50) values of CopA3 against pancreatic and hepatocellular cancer cells were 61.7 μM and 67.8 μM, respectively. The results indicate that CopA3 has potential in the treatments of pancreatic and hepatocellular cancers as well as microorganism infection disease.
Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-853, Korea.
In-Woo
IW
Kim
Soon-Ja
SJ
Kim
Yong-Nam
YN
Kwon
Eun-Young
EY
Yun
Mi-Young
MY
Ahn
Dong-Chul
DC
Kang
Jae-Sam
JS
Hwang
D000890:Anti-Infective Agents / Q000138:chemical synthesis* / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000138:chemical synthesis* / Q000235:genetics / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D020128:Inhibitory Concentration 50; D019476:Insect Proteins / Q000138:chemical synthesis* / Q000235:genetics / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests
D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D000970:Antineoplastic Agents; C583724:CopA3 peptide, Copris tripartitus; D019476:Insect Proteins; C570442:coprisin peptide, Copris tripartitus