Production and characterization of Clostridium perfringens recombinant β toxoid.
In this work, we produced and evaluated a vaccine based on a β toxoid of Clostridium perfringens type C produced in Escherichia coli (rBT). The non-toxic rBT was innocuous for mice and induced 14 IU mL(-1) of β antitoxin in rabbits, complying with the European Pharmacopeia and CFR9 - USDA guidelines.
Centro de Desenvolvimento Tecnológico - Biotecnologia, Universidade Federal de Pelotas, RS, Brazil.
Adalgisa
A
Milach
João Rodrigo Gil
JR
de los Santos
Carlos Gil
CG
Turnes
Angela Nunes
AN
Moreira
Ronnie Antunes
RA
de Assis
Felipe Masiero
FM
Salvarani
Francisco Carlos Faria
FC
Lobato
Fabricio Rochedo
FR
Conceição
D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics; D001428:Bacterial Vaccines / Q000096:biosynthesis* / Q000235:genetics / Q000633:toxicity; D003016:Clostridium perfringens / Q000276:immunology*; D004926:Escherichia coli; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D014121:Toxoids / Q000096:biosynthesis* / Q000235:genetics / Q000633:toxicity; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000096:biosynthesis* / Q000235:genetics / Q000633:toxicity
D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D014121:Toxoids; D014614:Vaccines, Synthetic