Granulocyte-colony stimulating factor promotes proliferation, migration and invasion in glioma cells.
Sustaining proliferative signaling is one of the hallmarks of cancer. Granulocyte-colony stimulating factor (G-CSF), a hematopoietic growth factor that controls proliferation and differentiation of neural stem cells, may contribute to the development of glioma. In the present study, we evaluated the expression of G-CSF and its receptor (G-CSFR) in various grades of glioma samples and primary cell culture derived from a glioblastoma patient as well as different human glioma cell lines. We showed that G-CSF and G-CSFR were widely expressed by glioma samples, primary glioma cell culture and glioma cell lines. The expression levels of G-CSF and G-CSFR were not significantly different between different grades of glioma. G-CSF promoted proliferation, migration and invasion of glioma cells. Neutralization of G-CSFR with its antibody inhibited growth and metastasis of glioma cells in vitro. We also showed that activation of signal transducer and activator transcription 3 (STAT 3) as well as expression of several of its downstream effectors was regulated by G-CSF. Taken together, the present results suggest that G-CSF contributes to glioma progression that may be linked to glioma genesis and recurrence.
Department of Neurosurgery, Qi Lu Hospital, Shandong University, Jinan, China.
Juntao
J
Wang
Linli
L
Yao
Shidou
S
Zhao
Xiaodan
X
Zhang
Junyi
J
Yin
Yanmin
Y
Zhang
Xueran
X
Chen
Ming
M
Gao
Eng-Ang
EA
Ling
Aijun
A
Hao
Gang
G
Li
D001921:Brain / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D016543:Central Nervous System Neoplasms / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology*; D015972:Gene Expression Regulation, Neoplastic; D005909:Glioblastoma / Q000473:pathology; D005910:Glioma / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology*; D016179:Granulocyte Colony-Stimulating Factor / Q000235:genetics* / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D060787:Neoplasm Grading; D010766:Phosphorylation / Q000187:drug effects; D016188:Receptors, Granulocyte Colony-Stimulating Factor / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism / Q000494:pharmacology; D012016:Reference Values; D050796:STAT3 Transcription Factor / Q000378:metabolism; D015398:Signal Transduction; D014407:Tumor Cells, Cultured
D016188:Receptors, Granulocyte Colony-Stimulating Factor; D011994:Recombinant Proteins; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D016179:Granulocyte Colony-Stimulating Factor