[Adenosine triphosphate-binding gene messenger ribonucleic acid expression in brains of drug-resistant epileptics].
OBJECTIVE
To explore the expression of ATP (adenosine triphosphate)-binding gene mRNA (messenger ribonucleic acid) in brain tissues of patients with drug-resistant epilepsy and understand their biological characteristics.


METHODS
The postoperative brain tissues were collected randomly from 40 patients with drug-refractory epilepsy. Based on the expected results of complementary DNA (cDNA) microarray, reverse transcription-polymerase chain reaction (RT-PCR) was used to evaluate ATP-binding gene mRNA expression in brain tissues of 40 patients with drug-resistant epilepsy and 8 normal controls.


RESULTS
As compared with 8 normal control cases, the candidate genes of ATP-binding mRNA were higherly expressed in the brain tissues of patients with drug-resistant epilepsy. The rates of Cy5/Cy3 were CDC2L5 2.159, ROCK1 2.538, STCH 2.106, OXSR1 3.791 and ABCB6 2.583 respectively. And the results of RT-PCR corresponded with those of cDNA chip.


CONCLUSIONS
The significant neuronal up-regulation of ATP-binding gene mRNA suggests that abnormal energy metabolism exist in brain tissues of patients with drug-resistant epilepsy. And the apoptosis of neurons and synaptic plasticity caused by abnormal energy metabolism may play an important role in the occurrence and development of patients with drug-resistant epilepsy.
Department of Neurology, First Affiliated Hospital, Chongqing Medical University, Chongqing Key Laboratories of Neurology, Chongqing 400016, China.
Pu
P
Han
Xue-Feng
XF
Wang
Liang
L
Wang
D000255:Adenosine Triphosphate / Q000378:metabolism; D001921:Brain / Q000378:metabolism; D004827:Epilepsy / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012333:RNA, Messenger; D000255:Adenosine Triphosphate