A novel biosensor for evaluation of apoptotic or necrotic effects of nitrogen dioxide during acute pancreatitis in rat.
The direct and accurate estimation of nitric dioxide levels is an extremely laborious and technically demanding procedure in the molecular diagnostics of inflammatory processes. The aim of this work is to demonstrate that a stop-flow technique utilizing a specific spectroscopic biosensor can be used for detection of nanomolar quantities of NO(2) in biological milieu. The use of novel compound cis-[Cr(C(2)O(4))(AaraNH(2))(OH(2))(2)](+) increases NO(2) estimation accuracy by slowing down the rate of NO(2) uptake. In this study, an animal model of pancreatitis, where nitrosative stress is induced by either 3g/kg bw or 1.5 g/kg bw dose of L-arginine, was used. Biochemical parameters and morphological characteristics of acute pancreatitis were monitored, specifically assessing pancreatic acinar cell death mode, NO(2) generation and cellular glutathione level. The severity of the process correlated positively with NO(2) levels in pancreatic acinar cell cytosol samples, and negatively with cellular glutathione levels.
Department of General and Inorganic Chemistry, University of Gdańsk, Sobieskiego 18/19, 80-952 Gdańsk, Poland. dagmara@chem.univ.gda.pl
Dagmara
D
Jacewicz
Aleksandra
A
Dabrowska
Dariusz
D
Wyrzykowski
Joanna
J
Pranczk
Michal
M
Wozniak
Jolanta
J
Kubasik-Juraniec
Narcyz
N
Knap
Kamila
K
Siedlecka
Alexander J
AJ
Neuwelt
Lech
L
Chmurzynski
D000208:Acute Disease; D000818:Animals; D017209:Apoptosis; D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D017022:Flow Injection Analysis / Q000295:instrumentation*; D008297:Male; D009336:Necrosis / Q000378:metabolism; D009585:Nitrogen Dioxide / Q000032:analysis / Q000378:metabolism*; D010195:Pancreatitis / Q000378:metabolism* / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar
D009585:Nitrogen Dioxide