Effective components screening and anti-myocardial infarction mechanism study of the Chinese medicine NSLF6 based on "system to system" mode.
BACKGROUND
Shuanglong formula (SLF), a Chinese medicine composed of panax ginseng and salvia miltiorrhiza exhibited significant effect in the treatment of myocardial infarction (MI) in clinical. Because of the complex nature and lack of stringent quality control, it's difficult to explain the action mechanism of SLF.


METHODS
In this study, we present a "system to system" (S2S) mode. Based on this mode, SLF was simplified successively through bioactivity-guided screening to achieve an optimized minimal phytochemical composition (new formula NSLF6) while maintaining its curative effect for MI.


RESULTS
Pharmacological test combining with the study of systems biology show that NSLF6 has activity for treatment MI through synergistic therapeutic efficacies between total ginsenosides and total salvianolic acids via promoting cardiac cell regeneration and myocardial angiogenesis, antagonistic myocardial cell oxidative damage.


CONCLUSIONS
The present S2S mode may be an effective way for the discovery of new composite drugs from traditional medicines.
Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Tsinghua University, Beijing 100084, China. luoga@tsinghua.edu.cn.
Qiong-Lin
QL
Liang
Xiao-Ping
XP
Liang
Yi-Ming
YM
Wang
Yuan-Yuan
YY
Xie
Rong-Li
RL
Zhang
Xi
X
Chen
Rong
R
Gao
Yi-Jun
YJ
Cheng
Jun
J
Wu
Qing-Bo
QB
Xu
Qing-Zhong
QZ
Xiao
Xue
X
Li
Shu-Feng
SF
Lv
Xue-Mei
XM
Fan
Hong-Yang
HY
Zhang
Qing-Li
QL
Zhang
Guo-An
GA
Luo
D000818:Animals; D002463:Cell Membrane Permeability / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003402:Creatine Kinase / Q000097:blood; D016002:Discriminant Analysis; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000187:drug effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007545:Isoproterenol; D007770:L-Lactate Dehydrogenase / Q000097:blood; D009203:Myocardial Infarction / Q000097:blood / Q000188:drug therapy* / Q000201:enzymology / Q000652:urine; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000473:pathology; D018919:Neovascularization, Physiologic / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D025341:Principal Component Analysis; D060066:Protein Interaction Maps; D051381:Rats; D015203:Reproducibility of Results; D049490:Systems Biology
D004365:Drugs, Chinese Herbal; D005230:Fatty Acids, Nonesterified; C572222:NSLF6 formula; C550108:shuanglong; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase; D007545:Isoproterenol