Two distinct broadly neutralizing antibody specificities of different clonal lineages in a single HIV-1-infected donor: implications for vaccine design.
Plasma from a small subset of subjects chronically infected with HIV-1 shows remarkable magnitude and breadth of neutralizing activity. From one of these individuals (CH0219), we isolated two broadly neutralizing antibodies (bnAbs), CH01 and VRC-CH31, from two clonal lineages of memory B cells with distinct specificities (variable loop 1 and 2 [V1V2] conformational specificity and CD4-binding site specificity, respectively) that recapitulate 95% of CH0219 serum neutralization breadth. These data provide proof of concept for an HIV-1 vaccine that aims to elicit bnAbs of multiple specificities.
Duke Human Vaccine Institute, Durham, North Carolina, USA. mattia.bonsignori@duke.edu
Mattia
M
Bonsignori
David C
DC
Montefiori
Xueling
X
Wu
Xi
X
Chen
Kwan-Ki
KK
Hwang
Chun-Yen
CY
Tsao
Daniel M
DM
Kozink
Robert J
RJ
Parks
Georgia D
GD
Tomaras
John A
JA
Crump
Saidi H
SH
Kapiga
Noel E
NE
Sam
Peter D
PD
Kwong
Thomas B
TB
Kepler
Hua-Xin
HX
Liao
John R
JR
Mascola
Barton F
BF
Haynes
D016915:AIDS Vaccines / Q000276:immunology; D057134:Antibodies, Neutralizing / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D000918:Antibody Specificity / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D001665:Binding Sites; D015704:CD4 Antigens / Q000737:chemistry / Q000378:metabolism; D000939:Epitopes / Q000737:chemistry; D015483:HIV Antibodies / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D015699:HIV Envelope Protein gp120 / Q000737:chemistry / Q000276:immunology; D015658:HIV Infections / Q000276:immunology; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000737:chemistry / Q000235:genetics; D009500:Neutralization Tests; D010802:Phylogeny
D016915:AIDS Vaccines; D057134:Antibodies, Neutralizing; D015704:CD4 Antigens; D000939:Epitopes; D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120; D007135:Immunoglobulin Variable Region