Immunohistochemical expression of p16, p21, p27 and cyclin D1 in oral nevi and melanoma.
The acquisition of abnormalities at G1/S is considered a crucial step in the genesis and progression of melanoma. The expression of cell cycle regulators has also been used in various neoplasms as an adjunct to diagnosis. The aim of this study was to compare the expression of p16, p21, p27 and cyclin D1 in oral nevi and melanomas. Expression of these cell cycle regulatory proteins was evaluated by immunohistochemistry in 51 oral melanocytic lesions, including 38 intramucosal nevi and 13 primary oral melanomas. p16 and p27 were highly expressed in intramucosal nevi, whereas p21 and cyclin D1 expression was higher in oral melanomas. The results indicate that p21 and cyclin D1 may be involved in the development of oral melanomas, and eventually they may be useful in the differential diagnoses of oral benign and malignant melanocytic lesions.
Oral Pathology Section, Department of Oral Diagnosis, Piracicaba Dental School, University of Campinas (UNICAMP), Av. Limeira 901, P.O. Box 52, 13414-903, Piracicaba, São Paulo, Brazil. augustodelima33@hotmail.com
Bruno Augusto Benevenuto
BA
de Andrade
Jorge Esquiche
JE
León
Román
R
Carlos
Wilson
W
Delgado-Azañero
Adalberto
A
Mosqueda-Taylor
Oslei Paes
OP
de Almeida
D000293:Adolescent; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D019938:Cyclin D1 / Q000032:analysis / Q000096:biosynthesis; D019941:Cyclin-Dependent Kinase Inhibitor p16; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000032:analysis / Q000096:biosynthesis; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000032:analysis / Q000096:biosynthesis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008545:Melanoma / Q000175:diagnosis / Q000378:metabolism*; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000175:diagnosis / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000032:analysis / Q000096:biosynthesis; D009506:Nevus / Q000175:diagnosis / Q000378:metabolism*; D055815:Young Adult
D014408:Biomarkers, Tumor; C530104:CCND1 protein, human; C493956:CDKN1A protein, human; C000614131:CDKN2A protein, human; D019941:Cyclin-Dependent Kinase Inhibitor p16; D050759:Cyclin-Dependent Kinase Inhibitor p21; D009363:Neoplasm Proteins; D019938:Cyclin D1; D050760:Cyclin-Dependent Kinase Inhibitor p27