Molecular characterization, polymorphism and association of porcine MYST2 gene.
MYST histone acetyltransferase 2 (MYST2) is an important reproduction related gene. In this study, we cloned the full-length cDNA sequence of porcine MYST2 gene through the rapid amplification of cDNA ends method. The porcine MYST2 gene encodes a protein of 611 amino acids which shares high homology with the MYST2 of six species: cattle (99%), rabbit (99%), human (99%), rat (99%), mouse (99%) and chicken (98%).The open reading frame of this gene is structured in 15 exons and 14 introns as revealed by computer-assisted analysis. The phylogenetic analysis revealed that the porcine MYST2 gene has a closer genetic distance with the MYST2 gene of cattle. PCR-RFLP was established to detect the GU373686:c.2872G > A substitution of porcine MYST2 gene mRNA and association of this mutation with litter size traits was assessed in Large White (n = 200) and Landrace (n = 200) pig populations. Results demonstrated that this polymorphic locus was significantly associated with the litter size of all parities in Large White sows and Landrace sows. These data serve as a foundation for further insight into this porcine gene.
Chongqing Key Laboratory of Pig Industry Sciences, Chongqing Academy of Animal Sciences, Rongchang, 402460 Chongqing, China.
Wang Jin
WJ
Yong
Lan
L
Jing
Zhao
Z
Jiugang
Chen
C
Lei
Liu
L
Yonggang
D000818:Animals; D018076:DNA, Complementary; D005787:Gene Frequency; D051548:Histone Acetyltransferases / Q000235:genetics*; D008098:Litter Size / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide; D012333:RNA, Messenger / Q000235:genetics; D017422:Sequence Analysis, DNA; D034421:Sus scrofa / Q000235:genetics* / Q000502:physiology
D018076:DNA, Complementary; D012333:RNA, Messenger; D051548:Histone Acetyltransferases