Characterization of a novel mouse monoclonal antibody, clone 1E8.33,             highly specific for human procollagen 11A1, a tumor-associated stromal component.
A novel IgG1, κ mouse monoclonal antibody (clone 1E8.33) to human procollagen             11A1 has been generated. This antibody is poorly mutated, essentially in germ             line configuration; its complementarity determining regions (CDRs) are especially             rich in tyrosine and serine residues. The epitope recognized is encompassed in             the YNYGTMESYQTEAPR amino acid stretch within the variable region of human procollagen             11A1. Human procollagens 5A1 and 11A1 are very similar. However, this antibody             does not cross-react with human procollagen 5A1. In human breast tumors, only             the activated peritumoral myofibroblasts show a strong intracytoplasmic staining             with this antibody. As procollagen 11A1 is overexpressed in the stroma of human             tumors with desmoplastic reaction, this antibody represents a valuable tool for             diagnostic purposes.
Biotechnological and Biomedical Assays Unit, Technical-Scientific                     Services, Oviedo University, 33006 Oviedo, Spain.
Marcos
M
García-Ocaña
Fernando
F
Vázquez
Carmen
C
García-Pravia
Nelson
N
Fuentes-Martínez
Primitiva
P
Menéndez-Rodríguez
Florentino
F
Fresno-Forcelledo
Luis
L
Barneo-Serra
Jokin
J
Del Amo-Iribarren
Laureano
L
Simón-Buela
Juan R
JR
De Los Toyos
D000595:Amino Acid Sequence; D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived / Q000276:immunology*; D000918:Antibody Specificity; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D045744:Cell Line, Tumor; D024062:Collagen Type V / Q000276:immunology; D024161:Collagen Type XI / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D003429:Cross Reactions; D018604:Epitope Mapping; D005260:Female; D006801:Humans; D016056:Immunodominant Epitopes; D007074:Immunoglobulin G / Q000276:immunology*; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D058628:Myofibroblasts / Q000276:immunology* / Q000378:metabolism; D011347:Procollagen / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017154:Stromal Cells / Q000276:immunology* / Q000378:metabolism
D058846:Antibodies, Monoclonal, Murine-Derived; C530725:COL5A1 protein, human; D024062:Collagen Type V; D024161:Collagen Type XI; D016056:Immunodominant Epitopes; D007074:Immunoglobulin G; D011347:Procollagen; D012333:RNA, Messenger