Identification and characterization of genes related to the development of skeletal muscle in the Hainan black goat.
In total, 185 unigenes were identified from 380 clones of postnatal skeletal muscle of Hainan Black goats by suppression subtractive hybridization (SSH) technology. Most of the differentially expressed genes involved energy metabolism and muscle contraction. The expression of 19 genes was analyzed in the longissimus dorsi muscles of 2-, 6-, 12-, 24-month olds, and four gene expression patterns were found by hierarchical cluster analysis. Most genes in first expression pattern belonged to myofibrillar proteins and had higher expression levels at 2 months old; genes of the secondary expression pattern had higher expression levels at 12 months old; tropomyoain 1 (alpha) (TPM1) was classified into the third expression pattern, and its expression level showed decreases tendency as age increased. Tropomyoain 2 (beta) (TPM2) was classified into the third expression pattern, which had the opposite expression pattern against TPM1.
Tropical Crops Genetic Resources Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, Hainan, PR China.
Tie Shan
TS
Xu
Xiao Hui
XH
Zhang
Li Hong
LH
Gu
Han Lin
HL
Zhou
Guang
G
Rong
Wei Ping
WP
Sun
D000367:Age Factors; D000818:Animals; D016000:Cluster Analysis; D003285:Contractile Proteins / Q000235:genetics; D016326:Extracellular Matrix Proteins / Q000235:genetics; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D006041:Goats / Q000235:genetics*; D009124:Muscle Proteins / Q000235:genetics*; D018482:Muscle, Skeletal / Q000254:growth & development* / Q000378:metabolism; D000072260:RNA Splicing Factors
D003285:Contractile Proteins; D016326:Extracellular Matrix Proteins; D009124:Muscle Proteins; D000072260:RNA Splicing Factors; C016598:microfibrillar protein