[Effect of IL-17 on the expression of monocyte chemoattractant protein-1 in the cardiac myocytes].
OBJECTIVE
To investigate the effect of interleukin-17 (IL-17) on the expression of monocyte chemoattractant protein-1(MCP-1) in the primary cultured cardiac myocytes.


METHODS
The cardiac myocytes were isolated from neonatal mice by different adhesion method. The expressions of IL-17R and MCP-1 in the cardiac myocytes were evaluated by reverse transcription-polymerase chain reaction (RT-PCR). And the concentration of MCP-1 in the culture supernatant of the cardiac myocytes was detected by enzyme-linked immunosorbent assay (ELISA).


RESULTS
There was the expression of IL-17R in the cardiac myocytes. After stimulated by IL-17,  the expressions of MCP-1 in the cardiac myocytes were significantly increased in dose-dependent manner compared with that of culture medium control (P<0.05). The amount of MCP-1 mRNA in the cardiac myocytes was the highest at 4 h after stimulated by IL-17. And then the amount of MCP-1 mRNA in the cardiac myocytes began to descend. The concentrations of  MCP-1 in the culture supernatant of the cardiac myocytes increased in time-dependent manner and had significant differences with the control group (P<0.05).


CONCLUSIONS
The cardiac myocytes could express IL-17R. IL-17 could up-regulate the expression of MCP-1 in the cardiac myocytes,  which was associated with the dose and stimulation time of IL-17.
Department of Clinical Laboratory Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China. liuyueyan2005@163.com
Yan-yue
YY
Liu
Yan-yan
YY
Li
Shan-shan
SS
Wang
Yan
Y
Shen
D000818:Animals; D018932:Chemokine CCL2 / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D020381:Interleukin-17 / Q000494:pharmacology*; D051379:Mice; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism*; D061251:Primary Cell Culture; D053722:Receptors, Interleukin-17 / Q000235:genetics
D018932:Chemokine CCL2; C491673:Il17ra protein, mouse; D020381:Interleukin-17; D053722:Receptors, Interleukin-17