Patterning surface by site selective capture of biopolymer hydrogel beads.
This communication describes the fabrication of microstructured biopolymer surfaces by the site-selective capture of pectin hydrogel beads. A positively charged surface consisting of poly-L-lysine (PLL) was subjected to site-selective enzymatic degradation using patterned polydimethylsiloxane (PDMS) stamps covalently modified with trypsin, according to the recently described method. The patterned surface was used to capture ionically cross-linked pectin beads. The desired patterning of the hydrogel surfaces was generated by site-selective immobilization of these pectin beads. The ability of the hydrogels to be dried and swollen in water was assessed.
UR1268 Biopolymères Interactions Assemblages, INRA, F-44300 Nantes, France. aurelieguyomard@gmail.com
Aurélie
A
Guyomard-Lack
Céline
C
Moreau
Nicolas
N
Delorme
Mélanie
M
Marquis
Aiping
A
Fang
Jean-François
JF
Bardeau
Bernard
B
Cathala
D001672:Biocompatible Materials / Q000138:chemical synthesis*; D001704:Biopolymers / Q000737:chemistry*; D003890:Desiccation; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D020100:Hydrogels / Q000138:chemical synthesis*; D008422:Materials Testing; D044085:Microfluidics; D008856:Microscopy, Fluorescence; D010368:Pectins / Q000737:chemistry*; D011107:Polylysine / Q000737:chemistry*; D055672:Static Electricity; D013499:Surface Properties; D014357:Trypsin / Q000378:metabolism; D017155:Wettability
D001672:Biocompatible Materials; D001704:Biopolymers; D004129:Dimethylpolysiloxanes; D020100:Hydrogels; D011107:Polylysine; D010368:Pectins; D014357:Trypsin