Clinical and virological outcome of an infection with the Belgian equine arteritis virus strain 08P178.
Equine viral arteritis (EVA) is an infectious disease with variable clinical outcome. Outbreaks, causing important economic losses, are becoming more frequent. Currently, there is a shortage of pathogenesis studies performed with European strains. In the present study, eight seronegative ponies were experimentally inoculated with the Belgian strain of equine arteritis virus (EAV) 08P178 (EU-1 clade) and monitored daily for clinical signs of EVA. Nasopharyngeal swabs, ocular swabs, bronchoalveolar cells and blood were collected for virological and serological testing. Two ponies were euthanized at 3, 7, 14, and 28 days post infection (DPI). After necropsy, specimens were collected for virus titration and immunofluorescence. EVA symptoms such as fever and lymphadenomegaly were evident from 3 to 10 DPI. Virus was isolated in nasal secretions from 2 to 9 DPI and in bronchoalveolar cells from 3 to 7 DPI. A cell-associated viraemia was detected from 3 to 10 DPI. After replication in the respiratory tract and draining lymph nodes, EAV reached secondary target organs (high virus titers in internal organs sampled at 7 DPI). At 14 DPI, virus titers dropped drastically and, at 28 DPI, only tonsils were positive. Immunofluorescence revealed both individual and clustered EAV-infected cells. Antibodies were detected starting from 7 DPI. It can be concluded that the Belgian strain 08P178 is a European mildly virulent subtype. At present, most European EAV strain infections were thought to run a subclinical course. This study is a proof that mildly virulent European EAV strains do exist in the field.
Department of Virology, Parasitology and Immunology, Ghent University, Faculty of Veterinary Medicine, Salisburylaan 133, 9820 Merelbeke, Belgium.
Sabrina
S
Vairo
Annelies
A
Vandekerckhove
Lennert
L
Steukers
Sarah
S
Glorieux
Wim
W
Van den Broeck
Hans
H
Nauwynck
D000818:Animals; D018174:Arterivirus Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D001530:Belgium; D007240:Equartevirus / Q000302:isolation & purification / Q000472:pathogenicity*; D005260:Female; D006734:Horse Diseases / Q000473:pathology* / Q000821:virology; D006736:Horses / Q000276:immunology / Q000821:virology*; D056724:Immunity, Humoral; D008297:Male; D017201:Virus Shedding