Degradation of mouse NTE-related esterase by macroautophagy and the proteasome.
NTE-related esterase (NRE) is a novel endoplasmic reticulum-anchored lysophospholipase with high homology to neuropathy target esterase (NTE). However, little is known about the regulation of NRE protein. In the current study, we investigated the degradation pathways of mouse NRE (mNRE) in mammalian cells. Based on experiments with inhibitors and inducer of protein degradation pathways, we provide here the first evidence that mNRE is degraded by macroautophagy as well as by the proteasome. Moreover, the contribution of protein domains to the degradation of mNRE was investigated, which showed that the transmembrane and regulatory domain played a role in the degradation of mNRE by macroautophagy and the proteasome respectively. In contrast the C-terminal catalytic domain was not involved in both degradation pathways of mNRE. These findings showed for the first time that the degradation pathways in controlling mNRE quantity and may provide further insight into structure and regulation of mNRE.
Key Laboratory of Molecular Biology, College of Bio-information, Chongqing University of Posts and Telecommunications, Nan'an District, Chongqing, People's Republic of China. changpingan@yahoo.com.cn
Ping-An
PA
Chang
Yu-Ying
YY
Chen
Ding-Xin
DX
Long
Wen-Zhen
WZ
Qin
Xiao-Ling
XL
Mou
D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D001343:Autophagy; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D003513:Cycloheximide / Q000494:pharmacology; D049452:Green Fluorescent Proteins / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D007976:Leupeptins / Q000494:pharmacology; D051379:Mice; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D000081082:Phosphoinositide-3 Kinase Inhibitors; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D061988:Proteasome Inhibitors; D014176:Protein Biosynthesis / Q000187:drug effects; D017434:Protein Structure, Tertiary; D059748:Proteolysis; D011993:Recombinant Fusion Proteins / Q000378:metabolism
D000225:Adenine; D002265:Carboxylic Ester Hydrolases; D003513:Cycloheximide; D049452:Green Fluorescent Proteins; D007976:Leupeptins; D019869:Phosphatidylinositol 3-Kinases; D000081082:Phosphoinositide-3 Kinase Inhibitors; D046988:Proteasome Endopeptidase Complex; D061988:Proteasome Inhibitors; D011993:Recombinant Fusion Proteins; C063261:acetylleucyl-leucyl-norleucinal; C025946:3-methyladenine; C530968:Pnpla7 protein, mouse; C072553:benzyloxycarbonylleucyl-leucyl-leucine aldehyde