A non-aqueous solid phase extraction method for alkaloid enrichment and its application in the determination of hyoscyamine and scopolamine.
A non-aqueous solid phase extraction (SPE) method utilizing silica based strong cation exchange (SCX) was developed and optimized for the enrichment of alkaloids. In this method, silica based SCX SPE columns were used for the elimination of non-alkaloid compounds and the preconcentration of alkaloids from the extracts. Mass spectrometry was employed to analyze the alkaloid-enriched fraction, and results showed that the SPE method developed in this study was effective for the removal of non-alkaloids. Then, this pretreatment method was combined with high performance liquid chromatography for the quantification of scopolamine and hyoscyamine from Scopolia tangutica Maxim. The recoveries of scopolamine and (-)-hyoscyamine were 98.51% and 91.12%, respectively. Relative standard deviation values were 1.4% for scopolamine and 1.6% for (-)-hyoscyamine. The linearity was good in the 0.01-0.8 mg mL(-1) range for hyoscyamine and 0.01-0.4 mg mL(-1) range for scopolamine.
Key Lab of Separation Science for Analytical Chemistry, Key Lab of Natural Medicine, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Liaoning Province. Dalian, 116023, China.
Zhen
Z
Long
Chaoran
C
Wang
Zhimou
Z
Guo
Xiuli
X
Zhang
Lilly
L
Nordahl
Jing
J
Zeng
Jianguo
J
Zeng
Xinmiao
X
Liang
D000470:Alkaloids / Q000737:chemistry*; D001285:Atropine / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D010936:Plant Extracts / Q000737:chemistry; D012601:Scopolamine / Q000032:analysis*; D032603:Scopolia / Q000737:chemistry; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*
D000470:Alkaloids; D010936:Plant Extracts; D001285:Atropine; D012601:Scopolamine