Retinoblastoma 94 enhances radiation treatment of esophageal squamous cell carcinoma in vitro and in vivo.
We performed the study to investigate whether adenovirus-mediated retinoblastoma 94 (RB94) gene transfer could enhance radiation treatment of esophageal squamous cell carcinoma (ESCC) in vitro and in vivo. ESCC cells (Kyse150 cell line) were cultivated in vitro and tumors originated from the cell line were propagated as xenografts in nude mice. Treatment with Ad-RB94 and/or ionizing radiation (IR) was carried out both in vitro and in vivo with Ad-LacZ control vector and blank control. Cell viability, cell cycle distribution, cell apoptosis, tumor growth and transfected gene expression were evaluated and tumor degeneration was analyzed. The data of quantification real-time PCR assays and immunohistochemistry staining using RB antibody indicated that RB94 was efficiently transfected into Kyse150 cells. In vitro, data of cell growth assay indicated that treatment with Ad-RB94 improved radiation treatment of Kyse150 cells. Tumor xenograft studies, pathological analysis of H.E. staining and Ki67 staining suggested transfecting RB94 enhanced tumor regression induced by radiation treatment in vivo. In addition, data of Annexin V, TUNEL and cell cycle distribution assays proposed combination treatment effectively induced cell apoptosis and cell cycle arresting in G2/M phase. In conclusion, transferring RB94 gene by the adenoviral vector enhances radiation treatment of ESCC.
Institute of Radiation Medicine, Peking Union Medical College, Chinese Academy of Medical Sciences, China.
Heng
H
Zhang
Jin
J
Li
Yue-ying
YY
Wang
Ai-min
AM
Meng
Qiang
Q
Liu
Lei
L
Wang
Feng-hua
FH
Chen
Xiao-chun
XC
Wang
Zhi-bin
ZB
Zhai
Yue
Y
Fu
Qin
Q
Wang
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D017209:Apoptosis / Q000528:radiation effects; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology / Q000532:radiotherapy / Q000628:therapy*; D002453:Cell Cycle / Q000528:radiation effects; D045744:Cell Line, Tumor / Q000528:radiation effects; D003131:Combined Modality Therapy; D004938:Esophageal Neoplasms / Q000235:genetics / Q000473:pathology / Q000532:radiotherapy / Q000628:therapy*; D016161:Genes, Retinoblastoma; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000235:genetics / Q000627:therapeutic use*; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010446:Peptide Fragments / Q000235:genetics / Q000627:therapeutic use; D060888:Real-Time Polymerase Chain Reaction; D016160:Retinoblastoma Protein / Q000235:genetics / Q000627:therapeutic use*; D014162:Transfection; D023041:Xenograft Model Antitumor Assays
D010446:Peptide Fragments; D016160:Retinoblastoma Protein