LC analysis of coumestrol incorporated into topical lipid nanoemulsions.
A simple, rapid, and sensitive LC method to determine coumestrol incorporated in the lipid nanoemulsions was validated. The analyses were performed at room temperature on a reversed-phase C18 column using a mobile phase composed of methanol/water with 0.1% trifluoracetic acid (70:30, v/v) at 0.8 mL min(-1). The detection was carried out on a UV detector at 343 nm. The linearity, in the range of 0.1-6.0 microg/mL, presented a determination coefficient (r2) of 0.999, calculated by the least square method. No interferences of the oil core or the gelling excipients were detected. The R.S.D. values for intra- and inter-day precision experiments were lower than 2%. The recovery ranged from 99.42% to 100.72%. Finally, the proposed method was successfully applied to determine coumestrol incorporated in the proposed topical formulations.
Programa de Pós-Graduaçã em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
D F
DF
Argenta
C
C
Franco
L S
LS
Koester
V L
VL
Bassani
H F
HF
Teixeira
D000287:Administration, Topical; D000975:Antioxidants / Q000008:administration & dosage / Q000032:analysis*; D002138:Calibration; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid; D003375:Coumestrol / Q000008:administration & dosage / Q000032:analysis*; D004339:Drug Compounding; D004655:Emulsions; D004968:Estrogens, Non-Steroidal / Q000008:administration & dosage / Q000032:analysis*; D007202:Indicators and Reagents; D008055:Lipids; D000432:Methanol; D053758:Nanoparticles; D010636:Phenols / Q000032:analysis; D015203:Reproducibility of Results; D012997:Solvents
D000975:Antioxidants; D004655:Emulsions; D004968:Estrogens, Non-Steroidal; D007202:Indicators and Reagents; D008055:Lipids; D010636:Phenols; D012997:Solvents; D003375:Coumestrol; D000432:Methanol