Mutants of Lactobacillus plantarum ML11-11 deficient in co-aggregation with yeast exhibited reduced activities of mixed-species biofilm formation.
Lactic acid bacteria (LAB) mutants deficient in inter-species co-aggregation with yeast were spontaneously derived from Lactobacillus plantarum ML11-11, a significant mixed-species biofilm former in static co-cultures with budding yeasts. These non-co-aggregative mutants also showed significant decreases in mixed-species biofilm formation. These results suggest the important role of co-aggregation between LAB and yeast in mixed-species biofilm formation. Cell surface proteins obtained by 5 M LiCl extraction from the wild-type cells and non-co-aggregative mutant cells were analyzed by SDS-PAGE. There was an obvious difference in protein profiles. The protein band at 30 kDa was present abundantly in the wild-type cell surface fraction but was significantly decreased in the mutant cells. This band assuredly corresponded to the LAB surface factors that contribute to co-aggregation with yeasts.
Department of Food Bioscience and Biotechnology, College of Bioresource Sciences, Nihon University, Kanagawa, Japan.
Soichi
S
Furukawa
Natsumi
N
Nojima
Soma
S
Nozaka
Satoru
S
Hirayama
Ayumi
A
Satoh
Hirokazu
H
Ogihara
Yasushi
Y
Morinaga
D001426:Bacterial Proteins / Q000032:analysis; D018441:Biofilms / Q000254:growth & development*; D002448:Cell Adhesion; D018920:Coculture Techniques; D048191:Lactiplantibacillus plantarum / Q000166:cytology / Q000235:genetics*; D008565:Membrane Proteins / Q000032:analysis; D009154:Mutation; D040901:Proteomics; D004718:Saccharomycetales / Q000166:cytology*; D013045:Species Specificity
D001426:Bacterial Proteins; D008565:Membrane Proteins