Concanavalin A immobilized poly(ethylene glycol dimethacrylate) based affinity cryogel matrix and usability of invertase immobilization.
Concanavalin A (Con A) immobilized supermacroporous poly(ethylene glycol dimethacrylate) [poly(EGDMA)] monolithic cryogel column was prepared by radical cryocopolymerization of EGDMA as a monomer and N,N'-methylene-bisacrylamide as a crosslinker. Bioligand Con A was then immobilized by covalent binding onto poly(EGDMA) cryogel via glutaraldehyde activation [Con A-poly(EGDMA)]. Con A-poly(EGDMA) cryogel was characterized by swelling studies and scanning electron microscopy. The monolithic cryogel contained a continuous polymeric matrix having interconnected pores of 10-50 μm size. The equilibrium swelling degree of the cryogel was 15.01 g H₂O/g dry cryogel. Con A-poly(EGDMA) cryogel was used in the adsorption/desorption of invertase from aqueous solutions. The maximum amount of invertase adsorption from aqueous solution in acetate buffer was 55.45 mg/g polymer at pH 5.0. Con A-poly(EGDMA) cryogels were used for repetitive adsorption/desorption of invertase without noticeable loss in invertase adsorption capacity after 10 cycles.
Adnan Menderes University, Faculty of Science & Arts, Chemistry Department, Aydin, Turkey.
Murat
M
Uygun
Deniz Aktaş
DA
Uygun
Emir
E
Ozçalışkan
Sinan
S
Akgöl
Adil
A
Denizli
D000327:Adsorption; D002846:Chromatography, Affinity / Q000295:instrumentation* / Q000379:methods; D003208:Concanavalin A / Q000737:chemistry* / Q000378:metabolism; D059985:Cryogels / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008689:Methacrylates / Q000737:chemistry*; D009994:Osmolar Concentration; D011092:Polyethylene Glycols / Q000737:chemistry*; D016062:Porosity; D043324:beta-Fructofuranosidase / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism
D059985:Cryogels; D004800:Enzymes, Immobilized; D008689:Methacrylates; C421283:poly(ethylene glycol)-dimethacrylate; D003208:Concanavalin A; D011092:Polyethylene Glycols; D043324:beta-Fructofuranosidase