Aqueous extract of Centella asiatica promotes corneal epithelium wound healing in vitro.
BACKGROUND
Centella asiatica is a traditional herbal medicine that has been shown to have pharmacological effect on skin wound healing, and could be potential therapeutic agent for corneal epithelial wound healing.


OBJECTIVE
This study was done to evaluate the effects of Centella asiatica on the proliferation and migration of rabbit corneal epithelial (RCE) cells in the in vitro wound healing model.


METHODS
RCE cells were cultured with or without supplementation of Centella asiatica aqueous extract. Viability and proliferation of the RCE cells was determined by MTT assay and cell cycle was analyzed by flow cytometry. In vitro re-epithelization was studied by scratch assay and migration rate was evaluated quantitatively by image analyzer. Expression of corneal specific differentiation markers, CK12 and connexin 43, were studied via RT-PCR.


RESULTS
It was found that supplementation of Centella asiatica did not show any significant effect on the RCE cells proliferation at the concentration up to 500ppm, while at the concentration of 1000ppm significantly inhibited RCE cells proliferation (p<0.05). However, at the concentration up to 62.5ppm, RCE cells shows significant enhancement of migration rate compared to the control group (p<0.05). It was also found that the supplementation of Centella asiatica aqueous extract did not alter the expression of differentiation markers and cell cycle.


CONCLUSIONS
In conclusion, supplementation of Centella asiatica aqueous extract at low concentrations could be useful to promote corneal epithelium wound healing.
Tissue Engineering Centre, Universiti Kebangsaan Malaysia Medical Centre, 56000 Kuala Lumpur, Malaysia. ruszyidrus@gmail.com
Bt Hj Idrus
BH
Ruszymah
Shiplu Roy
SR
Chowdhury
Nur Azeanty Bt Abdul
NA
Manan
Ooi Sew
OS
Fong
Mohd Ilham
MI
Adenan
Aminuddin Bin
AB
Saim
D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D028041:Centella; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000187:drug effects*; D019573:Epithelium, Corneal / Q000166:cytology / Q000187:drug effects*; D005131:Eye Injuries / Q000188:drug therapy*; D008517:Phytotherapy; D010936:Plant Extracts; D011817:Rabbits; D014315:Triterpenes / Q000494:pharmacology* / Q000627:therapeutic use; D014945:Wound Healing / Q000187:drug effects*
C526146:Centella asiatica extract; D010936:Plant Extracts; D014315:Triterpenes