Micro- and nanostructured hydroxyapatite-collagen microcarriers for bone tissue-engineering applications.
Novel hydroxyapatite (HA)-collagen microcarriers (MCs) with different micro/nanostructures were developed for bone tissue-engineering applications. The MCs were fabricated via calcium phosphate cement (CPC) emulsion in oil. Collagen incorporation in the liquid phase of the CPC resulted in higher MC sphericity. The MCs consisted of a porous network of entangled hydroxyapatite crystals, formed as a result of the CPC setting reaction. The addition of collagen to the MCs, even in an amount as small as 0.8 wt%, resulted in an improved interaction with osteoblast-like Saos-2 cells. The micro/nanostructure and the surface texture of the MCs were further tailored by modifying the initial particle size of the CPC. A synergistic effect between the presence of collagen and the nanosized HA crystals was found, resulting in significantly enhanced alkaline phosphatase activity on the collagen-containing nanosized HA MCs.
Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Metallurgy, Technical University of Catalonia (UPC), Avda. Diagonal 647, E-08028, Barcelona, Spain.
R A
RA
Perez
G
G
Altankov
E
E
Jorge-Herrero
M P
MP
Ginebra
D000818:Animals; D001842:Bone and Bones / Q000187:drug effects*; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D003094:Collagen / Q000494:pharmacology*; D017886:Durapatite / Q000737:chemistry* / Q000494:pharmacology*; D006801:Humans; D008863:Microspheres; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*; D014961:X-Ray Diffraction
D003094:Collagen; D017886:Durapatite