Molecular cloning and functional characterization of Tibetan Porcine STING.
Tibetan pig is well known for its strong disease resistance. However, little is known about the molecular basis of its strong resistance to disease. Stimulator of interferon (IFN) genes (STING), also known as MPYS/MITA/ERIS/TMEM173, is an adaptor that functions downstream of RIG-I and MAVS and upstream of TBK1 and plays a critical role in type I IFN induction. Here we report the first cloning and characterization of STING gene from Tibetan pig. The entire open reading frame (ORF) of the Tibetan porcine STING is 1137 bp, with a higher degree of sequence similarity with Landrace pig (98%) and cattle (88%) than with chimpanzee (84%), human (83%) or mouse (77%). The predicted protein is composed of 378 amino acids and has 4 putative transmembrane domains. Real-time quantitative PCR analysis indicated that Tibetan pig STING mRNA was most abundant in the lung and heart. Overexpression of Tibetan porcine STING led to upregulation of IFN-β and IFN-stimulated gene 15 (ISG15) in porcine jejunal epithelial cell line IPEC-J2 cells. This is the first study investigating the biological role of STING in intestinal epithelial cells, which lays a foundation for the further study of STING in intestinal innate immunity.
Key Laboratory for Animal Disease-Resistance Nutrition of Sichuan Province, Institute of Animal Nutrition, Sichuan Agricultural University, Yaan, Sichuan 625014, China.
Zhiqing
Z
Huang
Key Laboratory for Animal Disease-Resistance Nutrition of Sichuan Province, Institute of Animal Nutrition, Sichuan Agricultural University, Yaan, Sichuan 625014, China.
Xiaoling
X
Chen
Key Laboratory for Animal Disease-Resistance Nutrition of Sichuan Province, Institute of Animal Nutrition, Sichuan Agricultural University, Yaan, Sichuan 625014, China.
Keying
K
Zhang
Key Laboratory for Animal Disease-Resistance Nutrition of Sichuan Province, Institute of Animal Nutrition, Sichuan Agricultural University, Yaan, Sichuan 625014, China.
Bing
B
Yu
Key Laboratory for Animal Disease-Resistance Nutrition of Sichuan Province, Institute of Animal Nutrition, Sichuan Agricultural University, Yaan, Sichuan 625014, China.
Xiangbing
X
Mao
Key Laboratory for Animal Disease-Resistance Nutrition of Sichuan Province, Institute of Animal Nutrition, Sichuan Agricultural University, Yaan, Sichuan 625014, China.
Ye
Y
Zhao
Key Laboratory for Animal Disease-Resistance Nutrition of Sichuan Province, Institute of Animal Nutrition, Sichuan Agricultural University, Yaan, Sichuan 625014, China.
Daiwen
D
Chen
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002460:Cell Line; D003001:Cloning, Molecular; D005260:Female; D006801:Humans; D007113:Immunity, Innate; D016899:Interferon-beta / Q000378:metabolism; D008565:Membrane Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013552:Swine; D018609:Tibet; D014018:Tissue Distribution; D014452:Ubiquitins / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects
D008565:Membrane Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D014452:Ubiquitins; D016899:Interferon-beta