An assessment of the effectiveness of 5-methylthioninhydrin within dual action reagents for latent fingerprint development on paper substrates.
A critical investigation of 5-methylthioninhydrin (5MTN) is presented as a 'dual action' formulation component for the development of latent finger marks on paper substrates. Preparation of a dual action reagent was performed by combining proportions of 5MTN and zinc chloride (ZnCl(2)) in a pre-mixed solution. Developed prints (deposited on filter paper substrates) could be subsequently visualised in both colour and fluorescence modes. Finger mark quality was graded using a quartered print approach for a number of reagent compositions to deliver an optimised formulation recipe. To fully appraise 5MTN in comparison to currently employed chemistries, this reagent was evaluated against three alternative amino acid selective reagents, ninhydrin, 1,8-diazafluorenone (DFO) and 1,2-indandione/ZnCl(2). Six common paper types were used for this purpose and split depletion finger marks from six donors were collected. Finger mark sets were also left for two days or two weeks to show the effect of ageing on development quality. For the first time, it was shown that 5MTN/ZnCl(2) is effective as a 'dual action' reagent under the United Kingdom climate conditions. However, results presented herein show that the existing recommended chemistries and the 1,2 indandione/ZnCl(2) process are all more effective than this new latent finger mark enhancement reagent. In a preliminary sequencing study, we show the effectiveness of the existing DFO-ninhydrin sequence over dual action reagents.
Department of Forensic Science & Drug Monitoring, King's College London, UK.
Nadia
N
Porpiglia
Stephen
S
Bleay
Lesley
L
Fitzgerald
Leon
L
Barron
D001372:Aza Compounds / Q000737:chemistry; D002712:Chlorides / Q000737:chemistry; D003878:Dermatoglyphics; D006801:Humans; D007189:Indans / Q000737:chemistry; D007202:Indicators and Reagents / Q000737:chemistry; D009555:Ninhydrin / Q000031:analogs & derivatives* / Q000737:chemistry*; D010209:Paper; D013048:Specimen Handling; D013050:Spectrometry, Fluorescence; D017967:Zinc Compounds / Q000737:chemistry
C416542:1,2-indandione; C406982:1,8-diazafluoren-one; D001372:Aza Compounds; D002712:Chlorides; D007189:Indans; D007202:Indicators and Reagents; D017967:Zinc Compounds; C016837:zinc chloride; D009555:Ninhydrin