The cytotoxic effect of essential oils from Origanum vulgare L. and/or Rosmarinus officinalis L. on Aeromonas hydrophila.
This study aimed to evaluate the antibacterial activities of the essential oils from Origanum vulgare L. (OV) and Rosmarinus officinalis L. (RO), both singly and in combination at sub-inhibitory concentrations (¼ MIC + ¼ MIC), against Aeromonas hydrophila and to investigate the possible mechanisms underlying these activities. Used singly (OV: 2.5 μL/mL; RO: 20 μL/mL) or in a mixture (OV: 0.625 μL/mL + RO: 5 μL/L), these essential oils led to a significant decrease (p<0.01) in bacterial viability after 24 h of exposure. A decrease in glucose consumption by A. hydrophila and release of cellular material were observed immediately after the addition of the essential oils, both singly and as a mixture, and continued for up to 6 h. Electron microscopy of cells exposed to the essential oils revealed severe changes in the plasma membrane, cytoplasmic appearance, and cell shape during the 6-h exposure period. OV and RO essential oils combined at sub-inhibitory concentrations could be rationally applied to inhibit the growth of A. hydrophila in food products, particularly minimally processed vegetables.
Laboratory of Food Microbiology, Federal Institute of Education, Science, and Technology of Pernambuco, Vitória de Santo Antão, Brazil.
Geíza Alves de
GA
Azerêdo
Tânia Lúcia Montenegro
TL
Stamford
Regina Celia Bressan Queiroz de
RC
Figueiredo
Evandro Leite de
EL
Souza
D016980:Aeromonas hydrophila / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure; D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D004357:Drug Synergism; D005516:Food Microbiology; D005947:Glucose / Q000378:metabolism; D009822:Oils, Volatile / Q000302:isolation & purification / Q000494:pharmacology*; D031341:Origanum / Q000737:chemistry*; D010938:Plant Oils / Q000302:isolation & purification / Q000494:pharmacology*; D027542:Rosmarinus / Q000737:chemistry*; D013997:Time Factors
D000900:Anti-Bacterial Agents; D009822:Oils, Volatile; D010938:Plant Oils; D005947:Glucose