Evaluation of the relative biological effectiveness of carbon ion beams in the cerebellum using the rat organotypic slice culture system.
To clarify the relative biological effectiveness (RBE) values of carbon ion (C) beams in normal brain tissues, a rat organotypic slice culture system was used. The cerebellum was dissected from 10-day-old Wistar rats, cut parasagittally into approximately 600-µm-thick slices and cultivated using a membrane-based culture system with a liquid-air interface. Slices were irradiated with 140 kV X-rays and 18.3 MeV/amu C-beams (linear energy transfer = 108 keV/µm). After irradiation, the slices were evaluated histopathologically using hematoxylin and eosin staining, and apoptosis was quantified using the TdT-mediated dUTP-biotin nick-end labeling (TUNEL) assay. Disorganization of the external granule cell layer (EGL) and apoptosis of the external granule cells (EGCs) were induced within 24 h after exposure to doses of more than 5 Gy from C-beams and X-rays. In the early postnatal cerebellum, morphological changes following exposure to C-beams were similar to those following exposure to X-rays. The RBEs values of C-beams using the EGL disorganization and the EGC TUNEL index endpoints ranged from 1.4 to 1.5. This system represents a useful model for assaying the biological effects of radiation on the brain, especially physiological and time-dependent phenomena.
Gunma University Heavy Ion Medical Center, Maebashi, Gunma, Japan.
Yukari
Y
Yoshida
Yoshiyuki
Y
Suzuki
Wael S
WS
Al-Jahdari
Nobuyuki
N
Hamada
Tomoo
T
Funayama
Katsuyuki
K
Shirai
Hiroyuki
H
Katoh
Tetsuya
T
Sakashita
Yasuhiko
Y
Kobayashi
Takashi
T
Nakano
D000818:Animals; D017209:Apoptosis / Q000528:radiation effects; D002244:Carbon; D002531:Cerebellum / Q000166:cytology / Q000254:growth & development / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays / Q000009:adverse effects; D020450:Heavy Ions / Q000009:adverse effects*; D020287:In Situ Nick-End Labeling; D018499:Linear Energy Transfer; D017628:Microglia / Q000528:radiation effects; D009474:Neurons / Q000528:radiation effects; D009924:Organ Culture Techniques; D051381:Rats; D017208:Rats, Wistar; D012062:Relative Biological Effectiveness
D002244:Carbon