Inlaid Nd-substituted bismuth titanate nanoplates for protein immobilization and Nd-controlled electrochemical properties.
Neodymium (Nd) substituted bismuth titanate (Bi(4-x)Nd(x)Ti(3)O(12), BNTO-x) nanoplates inlaid one another were prepared by sol-gel hydrothermal method, which was explored for protein immobilization and biosensor fabrication. Comparative experiments witnessed that Bi(3+) ions in bismuth titanate (Bi(4)Ti(3)O(12), BTO) were successfully substituted with Nd(3+) ions, and the electrochemical properties of the Hb-Chi-BNTO biosensors closely depended on the Nd(3+) ion content. With increasing the Nd(3+) doping content, the electrochemical performance of the Hb-Chi-BNTO-x biosensors showed regularly variable. Moreover, compared with the Hb-Chi-BTO and other Hb-Chi-BNTO-x biosensors, the Hb-Chi-BNTO-0.85 biosensor had more excellent electrochemical and electrocatalytic properties such as stronger redox peak currents (approximately three-fold), smaller peak-to-peak separation (50 mV), larger heterogeneous electron transfer rate (14.1 ± 3.8s(-1)), higher surface concentration of electroactive redox protein (about 8.16 × 10(-11)mol/cm(2)), and better reproducibility and stability. The Nd-depended electrochemical properties of the Hb-Chi-BNTO biosensors may open up a new idea for designing third-generation electrochemical biosensors, and the BNTO-0.85-based biosensor is also expected to find potential applications in many areas such as biomedical, food, and environmental detection.
Key Lab for Organ Failure Research, Ministry of Education of China, Nanfang Hospital, Southern Medical University, Guangzhou 510515, PR China.
Huan
H
Guo
Cui
C
Ye
Hanchang
H
He
Zhiwu
Z
Chen
Jianqiang
J
Hu
Guanqi
G
Hu
Aiqing
A
Li
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D001729:Bismuth / Q000737:chemistry*; D002384:Catalysis; D004563:Electrochemistry; D004579:Electron Transport; D055767:Immobilized Proteins / Q000737:chemistry*; D009354:Neodymium / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D014025:Titanium / Q000737:chemistry*
D055767:Immobilized Proteins; C550183:bismuth titanate; D009354:Neodymium; D014025:Titanium; D001729:Bismuth