Improved skin test for differential diagnosis of bovine tuberculosis by the addition of Rv3020c-derived peptides.
A peptide cocktail derived from the mycobacterial antigens ESAT-6, CFP-10, and Rv3615c allowed differentiation between Mycobacterium bovis-infected and M. bovis bacillus Calmette-Guérin (BCG)-vaccinated cattle when used as a skin test reagent for a "DIVA" test (i.e., a test capable of differentiating infected and uninfected vaccinated animals). Addition of the antigen Rv3020c improves the diagnostic sensitivity without compromising specificity in the face of BCG or Johne's disease vaccination.
TB Research Group, Animal Health and Veterinary Laboratories Agency, New Haw, Addlestone, Surrey, United Kingdom. gareth.j.jones@ahvla.gsi.gov.uk
Gareth J
GJ
Jones
Adam
A
Whelan
Derek
D
Clifford
Mick
M
Coad
H Martin
HM
Vordermeier
D000818:Animals; D000942:Antigens, Bacterial; D001500:BCG Vaccine / Q000276:immunology; D002417:Cattle; D003937:Diagnosis, Differential; D009163:Mycobacterium bovis / Q000276:immunology*; D010455:Peptides; D012680:Sensitivity and Specificity; D012882:Skin Tests / Q000379:methods; D014380:Tuberculosis, Bovine / Q000175:diagnosis* / Q000276:immunology; D014730:Veterinary Medicine / Q000379:methods*
D000942:Antigens, Bacterial; D001500:BCG Vaccine; D010455:Peptides