Molecular markers for detection and differentiation of Plasmodium falciparum and Plasmodium vivax in human blood samples by pyrosequencing.
PCR amplification coupled with pyrosequencing was used to measure molecular markers that could be used to detect and differentiate Plasmodium falciparum and Plasmodium vivax in human blood samples. The detection rates were in agreement with the results of Giemsa-stained film microscopy, which is the current gold standard for detection. This method provides an exciting alternative for malaria diagnosis.
Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen, Thailand.
Viraphong
V
Lulitanond
Pewpan M
PM
Intapan
Chairat
C
Tantrawatpan
Nipaporn
N
Sankuntaw
Oranuch
O
Sanpool
Penchom
P
Janwan
Wanchai
W
Maleewong
D001483:Base Sequence; D003937:Diagnosis, Differential; D017125:Genes, Protozoan; D005819:Genetic Markers; D006801:Humans; D016778:Malaria, Falciparum / Q000097:blood / Q000175:diagnosis* / Q000469:parasitology; D016780:Malaria, Vivax / Q000097:blood / Q000175:diagnosis* / Q000469:parasitology; D008969:Molecular Sequence Data; D058889:Molecular Typing / Q000379:methods; D010963:Plasmodium falciparum / Q000235:genetics*; D010966:Plasmodium vivax / Q000235:genetics*; D054679:Ribosome Subunits, Small / Q000235:genetics*; D017422:Sequence Analysis, DNA
D005819:Genetic Markers