Evaluation of acetylcholinesterase in an animal model of maple syrup urine disease.
Maple syrup urine disease is an inherited metabolic disease predominantly characterized by neurological dysfunction. However, the mechanisms underlying the neuropathology of this disease are still not defined. Therefore, the aim of this study was to investigate the effect of acute and chronic administration of a branched-chain amino acids (BCAA) pool (leucine, isoleucine, and valine) on acetylcholinesterase (AChE) activity and gene expression in the brain and serum of rats and to assess if antioxidant treatment prevented the alterations induced by BCAA administration. Our results show that the acute administration of a BCAA pool in 10- and 30-day-old rats increases AChE activity in the cerebral cortex, striatum, hippocampus, and serum. Moreover, chronic administration of the BCAA pool also increases AChE activity in the structures studied, and antioxidant treatment prevents this increase. In addition, we show a significant decrease in the mRNA expression of AChE in the hippocampus following acute administration in 10- and 30-day-old rats. On the other hand, AChE expression increased significantly after chronic administration of the BCAA pool. Interestingly, the antioxidant treatment was able to prevent the increased AChE activity without altering AChE expression. In conclusion, the results from the present study demonstrate a marked increase in AChE activity in all brain structures following the administration of a BCAA pool. Moreover, the increased AChE activity is prevented by the coadministration of N-acetylcysteine and deferoxamine as antioxidants.
Laboratório de Bioenergética, Programa de Pós-Graduação em Ciências da Saúde, Universidade do Extremo Sul Catarinense, Av. Universitária, 1105, Criciúma 88806-000, Santa Catarina, Brazil.
Giselli
G
Scaini
Natália
N
de Rochi
Isabela C
IC
Jeremias
Pedro F
PF
Deroza
Alexandra I
AI
Zugno
Talita C B
TC
Pereira
Giovanna M T
GM
Oliveira
Luiza W
LW
Kist
Maurício R
MR
Bogo
Patrícia F
PF
Schuck
Gustavo C
GC
Ferreira
Emilio L
EL
Streck
D000110:Acetylcholinesterase / Q000097:blood* / Q000235:genetics; D000597:Amino Acids, Branched-Chain / Q000378:metabolism* / Q000633:toxicity; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D001923:Brain Chemistry / Q000187:drug effects / Q000502:physiology*; D004195:Disease Models, Animal; D008297:Male; D008375:Maple Syrup Urine Disease / Q000139:chemically induced / Q000188:drug therapy* / Q000201:enzymology*; D051381:Rats; D017208:Rats, Wistar
D000597:Amino Acids, Branched-Chain; D000975:Antioxidants; D000110:Acetylcholinesterase