A photosynthetic biosensor with enhanced electron transfer generation realized by laser printing technology.
One of the limits of current electrochemical biosensors is a lack of methods providing stable and highly efficient junctions between biomaterial and solid-state devices. This paper shows how laser-induced forward transfer (LIFT) can enable efficient electron transfer from photosynthetic biomaterial immobilized on screen-printed electrodes (SPE). The ideal pattern, in terms of photocurrent signal of thylakoid droplets giving a stable response signal with a current intensity of approximately 335 ± 13 nA for a thylakoid mass of 28 ± 4 ng, was selected. It is shown that the efficiency of energy production of a photosynthetic system can be strongly enhanced by the LIFT process, as demonstrated by use of the technique to construct an efficient and sensitive photosynthesis-based biosensor for detecting herbicides at nanomolar concentrations.
Institute of Crystallography, National Research Council, Rome, Italy. toulou_e@chemistry.uoc.gr
Eleftherios
E
Touloupakis
Christos
C
Boutopoulos
Katia
K
Buonasera
Ioanna
I
Zergioti
Maria Teresa
MT
Giardi
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004566:Electrodes; D006540:Herbicides / Q000032:analysis*; D010788:Photosynthesis; D010936:Plant Extracts / Q000737:chemistry*; D011327:Printing; D018724:Spinacia oleracea / Q000737:chemistry; D020524:Thylakoids / Q000737:chemistry*
D006540:Herbicides; D010936:Plant Extracts