[Changes and effects of significant membrane molecules on thymic T cell of aging mice induced by D-galactose].
OBJECTIVE
To establish subacute aging mice model by D-galactose and to explore the changes and effects of significant membrane molecules on thymic T cell.


METHODS
Female Kunming mice of 8 weeks old were injected with D-galactose of 12.5 mL/(kg.d) by subcutaneous in scruff for 42 days. The animals' living conditions and biological behaviors were observed everyday.SOD activities and MDA content of serum were measured to determine whether the aging model was successfully established.On the basis of successfully establishing aging model, detect the significant membrane molecules of thymic T cell by Immunofluorescence technique and Flow Cytometer.


RESULTS
During the 42 days, gradually, the model mice showed bending body, loose skin, slow action and so on.The activities of SOD in the serum were significantly decreased(P<0.01), and the content of MDA in the serum was significantly increased(P<0.01). The thymic naive T cell significant molecule, CD45RA was decreased(P<0.05). T cell activation-related molecules, CD28 and CD25 were both decreased(P<0.05), and PD-1 was significantly increased(P<0.01). The memory T cell significant molecule, CD196 was increased, but was not significantly compared to the control mice.


CONCLUSIONS
The D-galactose subacute aging mice model was successfully established.The naive and active T cell were decreased and the memory T cell was increased in the thymic of the aging.
Department of Immunology, Soochow University, Suzhou, China. zhangyj0815@126.com
Yan-jun
YJ
Zhang
Hua-ting
HT
Zhu
Sai-nan
SN
Huang
Xiu-qing
XQ
Xue
Yu-hua
YH
Qiu
D000375:Aging / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D005690:Galactose / Q000494:pharmacology; D007156:Immunologic Memory / Q000187:drug effects / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology; D008315:Malondialdehyde / Q000097:blood; D051379:Mice; D013482:Superoxide Dismutase / Q000097:blood; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D060168:Thymocytes / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism
D008315:Malondialdehyde; D013482:Superoxide Dismutase; D005690:Galactose