Humoral immune responses to Plasmodium vivax subtelomeric transmembrane proteins in Thailand.
Plasmodium vivax subtelomeric transmembrane protein (PvSTP) is a homolog of P. falciparum SURFIN4.2', a protein exposed on the parasite-infected erythrocyte (iE) surface, and is thus considered to be exposed on P. vivax-iE. Because antibodies targeting antigens located on the surface of P. falciparum-iE, such as P. falciparum erythrocyte membrane protein 1, play an important role in regulating the course of disease, we evaluated the presence of antibodies in P. vivax-infected patients against two PvSTP paralogs, PvSTP1 and PvSTP2. Recombinant proteins corresponding to cysteine-rich domain (CRD) of the PvSTP extracellular region and the cytoplasmic region (CYT) were generated and used for the enzyme-linked immunosorbent assay. Plasma samples (n = 70) reacted positively with recombinant PvSTP1-CRD (40%), PvSTP1-CYT (31%), PvSTP2-CRD (27%), and PvSTP2-CYT (56%), suggesting that PvSTP1 and -2 are naturally immunogenic. Specific response against either PvSTP1 or PvSTP2 indicates the existence of specific antibodies for either PvSTP1 or -2.
Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Tippawan
T
Sungkapong
Richard
R
Culleton
Kazuhide
K
Yahata
Mayumi
M
Tachibana
Ronatrai
R
Ruengveerayuth
Rachanee
R
Udomsangpetch
Motomi
M
Torii
Takafumi
T
Tsuboi
Jetsumon
J
Sattabongkot
Osamu
O
Kaneko
Kesinee
K
Chotivanich
D000293:Adolescent; D000328:Adult; D000913:Antibodies, Protozoan / Q000276:immunology; D000962:Antimalarials / Q000627:therapeutic use; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D056724:Immunity, Humoral; D016780:Malaria, Vivax / Q000188:drug therapy / Q000276:immunology*; D008297:Male; D008565:Membrane Proteins / Q000276:immunology*; D008875:Middle Aged; D010963:Plasmodium falciparum / Q000276:immunology; D010966:Plasmodium vivax / Q000276:immunology*; D015800:Protozoan Proteins / Q000276:immunology*; D018709:Statistics, Nonparametric; D013785:Thailand
D000913:Antibodies, Protozoan; D000962:Antimalarials; D008565:Membrane Proteins; D015800:Protozoan Proteins