Simple technique for RNA purification from mouse inner ear hair cells.
Obtaining a good quality of RNA from small population of cells remain an issue. Isolation for a special anatomic location such as inner ear placed in the temporal bone become a challenge, especially in terms of time needed for isolation of living tissue from the bone, which is a key factor to preserve the RNA. Due to limited accessibility to the technologies such as laser dissection, we present a simplified procedure for isolation of good quality of RNA from the inner ear for further studies.
Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland. marcinsz@man.poznan.pl
Marcin
M
Szaumkessel
Damian
D
Brauze
Małgorzata
M
Rydzanicz
Michał
M
Karlik
Krzysztof
K
Szyfter
Witold
W
Szyfter
Wróbel
W
Maciej
D000617:Aminoglycosides; D000818:Animals; D003638:Deafness / Q000139:chemically induced / Q000378:metabolism; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D020869:Gene Expression Profiling; D005819:Genetic Markers; D006199:Hair Cells, Auditory, Inner / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D009925:Organ of Corti / Q000166:cytology; D012313:RNA / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
D000617:Aminoglycosides; D005819:Genetic Markers; D012313:RNA