Method for protein subfractionation of cardiovascular tissues before DIGE analysis.
Difference gel electrophoresis (DIGE) (Electrophoresis 18, 2071-2077, 1997, 1) is widely used in cardiovascular research. However, the dynamic range limitations stemming from contaminating plasma proteins and highly abundant extracellular matrix components can make cardiovascular tissues difficult to analyze. Here we describe a novel methodology for biochemical subfractionation of cardiovascular tissues before DIGE analysis.
Cardiovascular Division, King's College London, London, UK.
Athanasios
A
Didangelos
Xiaoke
X
Yin
Manuel
M
Mayr
D053000:Analytic Sample Preparation Methods / Q000379:methods*; D002319:Cardiovascular System / Q000166:cytology*; D005591:Chemical Fractionation / Q000379:methods*; D006031:Glycosylation; D019791:Guanidine / Q000302:isolation & purification; D011506:Proteins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*; D040901:Proteomics; D057825:Two-Dimensional Difference Gel Electrophoresis / Q000379:methods*
D011506:Proteins; D019791:Guanidine