The entirely carbohydrate immunogen Tn-PS A1 induces a cancer cell selective immune response and cytokine IL-17.
The tumor-associated carbohydrate antigen/hapten Thomsen-nouveau (Tn; a-D-GalpNAc-ONH2) was conjugated to a zwitterionic capsular polysaccharide, PS A1, from commensal anaerobe Bacteroides fragilis ATCC 25285/NCTC 9343 for the development of an entirely carbohydrate cancer vaccine construct and probed for immunogenicity. This communication discloses that murine anti-Tn IgG3 antibodies both bind to and recognize human tumor cells that display the Tn hapten. Furthermore, the sera from immunization of mice with Tn-PS A1 contain cytokine interleukin 17 (IL-17A), which is known to possess anti-tumor function and represents a striking difference to an IL-2, and IL-6 profile obtained with anti-PS A1 sera.
Department of Chemistry, Wayne State University, 5100 Cass Avenue, Detroit, MI 48202, USA.
Ravindra A
RA
De Silva
Dananjaya K
DK
Appulage
Halina
H
Pietraszkiewicz
Kevin R
KR
Bobbitt
Joe
J
Media
JiaJiu
J
Shaw
Fred A
FA
Valeriote
Peter R
PR
Andreana
D000818:Animals; D000951:Antigens, Neoplasm / Q000276:immunology*; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000276:immunology*; D001441:Bacteroides fragilis / Q000276:immunology*; D001943:Breast Neoplasms / Q000188:drug therapy / Q000276:immunology* / Q000473:pathology; D019496:Cancer Vaccines / Q000276:immunology*; D002241:Carbohydrates / Q000737:chemistry / Q000276:immunology; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007109:Immunity; D007114:Immunization; D007074:Immunoglobulin G / Q000378:metabolism; D020381:Interleukin-17 / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D011135:Polysaccharides, Bacterial / Q000008:administration & dosage / Q000737:chemistry / Q000235:genetics / Q000276:immunology
D000951:Antigens, Neoplasm; D015295:Antigens, Tumor-Associated, Carbohydrate; D019496:Cancer Vaccines; D002241:Carbohydrates; D007106:Immune Sera; D007074:Immunoglobulin G; D020381:Interleukin-17; D011135:Polysaccharides, Bacterial; C040286:Tn antigen