title: Spike protein VP8* of human rotavirus recognizes histo-blood group antigens in a type-specific manner.
abstract: Rotaviruses (RVs), an important cause of severe diarrhea in children, have been found to recognize sialic acid as receptors for host cell attachment. While a few animal RVs (of P[1], P[2], P[3], and P[7]) are sialidase sensitive, human RVs and the majority of animal RVs are sialidase insensitive. In this study, we demonstrated that the surface spike protein VP8* of the major P genotypes of human RVs interacts with the secretor histo-blood group antigens (HBGAs). Strains of the P[4] and P[8] genotypes shared reactivity with the common antigens of Lewis b (Le(b)) and H type 1, while strains of the P[6] genotype bound the H type 1 antigen only. The bindings between recombinant VP8* and human saliva, milk, or synthetic HBGA oligosaccharides were demonstrated, which was confirmed by blockade of the bindings by monoclonal antibodies (MAbs) specific to Le(b) and/or H type 1. In addition, specific binding activities were observed when triple-layered particles of a P[8] (Wa) RV were tested. Our results suggest that the spike protein VP8* of RVs is involved in the recognition of human HBGAs that may function as ligands or receptors for RV attachment to host cells.
authors.affiliation: Division of Infectious Diseases, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA.
authors.forename: Pengwei
authors.initials: P
authors.lastname: Huang
authors.forename: Ming
authors.initials: M
authors.lastname: Xia
authors.forename: Ming
authors.initials: M
authors.lastname: Tan
authors.forename: Weiming
authors.initials: W
authors.lastname: Zhong
authors.forename: Chao
authors.initials: C
authors.lastname: Wei
authors.forename: Leyi
authors.initials: L
authors.lastname: Wang
authors.forename: Ardythe
authors.initials: A
authors.lastname: Morrow
authors.forename: Xi
authors.initials: X
authors.lastname: Jiang
mesh_terms: D001789:Blood Group Antigens / Q000378:metabolism*; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism; D054884:Host-Pathogen Interactions; D006801:Humans; D007983:Lewis Blood Group Antigens / Q000378:metabolism; D008895:Milk, Human / Q000378:metabolism; D029322:Norovirus / Q000235:genetics / Q000378:metabolism; D009844:Oligosaccharides / Q000378:metabolism; D011485:Protein Binding; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012401:Rotavirus / Q000235:genetics / Q000378:metabolism*; D012463:Saliva / Q000378:metabolism; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000378:metabolism*; D014771:Virion / Q000378:metabolism
chemical_list: D001789:Blood Group Antigens; D036022:Capsid Proteins; D007983:Lewis Blood Group Antigens; D009844:Oligosaccharides; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D017361:Viral Nonstructural Proteins; C079234:NS35 protein, rotavirus