title: Development and validation of automated SPE-HPLC-MS/MS methods for the quantification of asenapine, a new antipsychotic agent, and its two major metabolites in human urine.
abstract: To support the evaluation of the pharmacokinetic parameters of asenapine (ASE) in urine, we developed and validated online solid-phase extraction high-performance liquid chromatography methods with tandem mass spectrometry detection (SPE-LC-MS/MS) for the quantification of ASE and two of its major metabolites, N-desmethylasenapine (DMA) and asenapine-N⁺-glucuronide (ASG). The linearity in human urine was found acceptable for quantification in a concentration range of 0.500-100 ng/mL for ASE and DMA and 10.0-3000 ng/mL for ASG, respectively.
authors.affiliation: QPS Netherlands, Petrus Campersingel 123, 9713, AG, Groningen, The Netherlands. theo.de.boer@qps.com
authors.forename: Theo
authors.initials: T
authors.lastname: de Boer
authors.forename: Erik
authors.initials: E
authors.lastname: Meulman
authors.forename: Henri
authors.initials: H
authors.lastname: Meijering
authors.forename: Jaap
authors.initials: J
authors.lastname: Wieling
authors.forename: Peter
authors.initials: P
authors.lastname: Dogterom
authors.forename: Holger
authors.initials: H
authors.lastname: Lass
mesh_terms: D014150:Antipsychotic Agents / Q000493:pharmacokinetics / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003986:Dibenzocycloheptenes; D004311:Double-Blind Method; D006576:Heterocyclic Compounds, 4 or More Rings / Q000493:pharmacokinetics / Q000652:urine*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D014150:Antipsychotic Agents; D003986:Dibenzocycloheptenes; D006576:Heterocyclic Compounds, 4 or More Rings; C522667:asenapine