title: Nitration of the pollen allergen bet v 1.0101 enhances the presentation of bet v 1-derived peptides by HLA-DR on human dendritic cells.
abstract: Nitration of pollen derived allergens can occur by NO(2) and ozone in polluted air and it has already been shown that nitrated major birch (Betula verrucosa) pollen allergen Bet v 1.0101 (Bet v 1) exhibits an increased potency to trigger an immune response. However, the mechanisms by which nitration might contribute to the induction of allergy are still unknown. In this study, we assessed the effect of chemically induced nitration of Bet v 1 on the generation of HLA-DR associated peptides. Human dendritic cells were loaded with unmodified Bet v 1 or nitrated Bet v 1, and the naturally processed HLA-DR associated peptides were subsequently identified by liquid chromatography-mass spectrometry. Nitration of Bet v 1 resulted in enhanced presentation of allergen-derived HLA-DR-associated peptides. Both the copy number of Bet v 1 derived peptides as well as the number of nested clusters was increased. Our study shows that nitration of Bet v 1 alters antigen processing and presentation via HLA-DR, by enhancing both the quality and the quantity of the Bet v 1-specific peptide repertoire. These findings indicate that air pollution can contribute to allergic diseases and might also shed light on the analogous events concerning the nitration of self-proteins.
authors.affiliation: Immunosafety, Non-Clinical Drug Safety, F. Hoffmann-La Roche Ltd, Basel, Switzerland.
authors.forename: Anette C
authors.initials: AC
authors.lastname: Karle
authors.forename: Gertie J
authors.initials: GJ
authors.lastname: Oostingh
authors.forename: Sonja
authors.initials: S
authors.lastname: Mutschlechner
authors.forename: Fatima
authors.initials: F
authors.lastname: Ferreira
authors.forename: Peter
authors.initials: P
authors.lastname: Lackner
authors.forename: Barbara
authors.initials: B
authors.lastname: Bohle
authors.forename: Gottfried F
authors.initials: GF
authors.lastname: Fischer
authors.forename: Anne B
authors.initials: AB
authors.lastname: Vogt
authors.forename: Albert
authors.initials: A
authors.lastname: Duschl
mesh_terms: D000397:Air Pollution / Q000009:adverse effects; D000485:Allergens / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D017951:Antigen Presentation / Q000276:immunology*; D052179:Antigens, Plant / Q000378:metabolism*; D029662:Betula; D003713:Dendritic Cells / Q000276:immunology*; D006684:HLA-DR Antigens / Q000276:immunology*; D006801:Humans; D006967:Hypersensitivity / Q000209:etiology; D009566:Nitrates / Q000378:metabolism; D010455:Peptides; D011058:Pollen / Q000276:immunology
chemical_list: D000485:Allergens; D052179:Antigens, Plant; D006684:HLA-DR Antigens; D009566:Nitrates; D010455:Peptides; C061312:Bet v 1 allergen, Betula