title: Non random distribution of lesions induced by deoxyribonuclease I in human chromosomes.
abstract: We studied the action of deoxyribonuclease I on human lymphocytes in order to determine the localization of the DNAase induced aberrations. Our results indicate a non-random distribution of the lesions on chromosome regions which may reflect a differential pattern of sensitivity to the enzyme. Furthermore we observed a correspondence between the preferential DNAase induced breaks and fragile sites that are expressed in lymphocytes maintained in medium without folic acid. A possible interpretation of our findings is that the accessibility to DNAase and/or the efficiency of the repair systems depend on the chromatin structure that influences also the expression of some common fragile sites.
authors.affiliation: Instituto di Genetica Biochimica ed Evoluzionistica del C.N.R., Via Abbiategrasso 207, I-27100, Pavia, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Nuzzo
authors.forename: A
authors.initials: A
authors.lastname: Casati
authors.forename: E
authors.initials: E
authors.lastname: Raimondi