title: Antidiabetic effect of Eclipta alba associated with the inhibition of alpha-glucosidase and aldose reductase.
abstract: The antidiabetic effect of the ethanolic extract of Eclipta alba (EEA) on hyperglycaemia and diabetic nephropathy was investigated in streptozotocin-induced diabetic rats. Single-dose treatment of EEA to streptozotocin-induced diabetic rats lowered the blood glucose level by 17.6% (p < 0.05) at 250 mg kg(-1) dose after 5 h post oral administration. Treatment of animals after 10 weeks of STZ-treatment with EEA (250 mg kg(-1)) for 21 days significantly reduced the elevated levels of blood glucose, %HbA1C, urea, uric acid and creatinine, and significantly increased the depressed serum insulin level. The extract exerted a significant inhibitory effect on alpha-glucosidase in a noncompetitive manner with an IC(50) value of around 54 µg mL(-1) and was found inhibitory to eye lens aldose reductase with an IC(50) value of around 4.5 µg mL(-1). The results suggest that EEA possesses antidiabetic effect associated with alpha-glucosidase and aldose reductase inhibition.
authors.affiliation: Division of Biochemistry, Central Drug Research Institute, Council of Scientific and Industrial Research, Lucknow 226 001, Uttar Pradesh, India.
authors.forename: Natasha
authors.initials: N
authors.lastname: Jaiswal
authors.forename: Vikram
authors.initials: V
authors.lastname: Bhatia
authors.forename: Swayam Prakash
authors.initials: SP
authors.lastname: Srivastava
authors.forename: Arvind Kumar
authors.initials: AK
authors.lastname: Srivastava
authors.forename: Akhilesh Kumar
authors.initials: AK
authors.lastname: Tamrakar
mesh_terms: D000284:Administration, Oral; D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors*; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003404:Creatinine / Q000097:blood; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D004305:Dose-Response Relationship, Drug; D036601:Eclipta / Q000737:chemistry*; D065089:Glycoside Hydrolase Inhibitors; D007004:Hypoglycemic Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D007194:India; D020128:Inhibitory Concentration 50; D007328:Insulin / Q000097:blood; D007908:Lens, Crystalline / Q000201:enzymology; D010936:Plant Extracts / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D014508:Urea / Q000097:blood; D014527:Uric Acid / Q000097:blood
chemical_list: D001786:Blood Glucose; D065089:Glycoside Hydrolase Inhibitors; D007004:Hypoglycemic Agents; D007328:Insulin; D010936:Plant Extracts; D014527:Uric Acid; D014508:Urea; D003404:Creatinine; D000449:Aldehyde Reductase