title: Possible involvement of maize Rop1 in the defence responses of plants to viral infection.
abstract: The expression of host genes can be altered during the process of viral infection. To investigate the viral infection-induced up-regulated gene expression changes of maize at different time intervals post-inoculation with Sugarcane mosaic virus (SCMV), a suppression subtractive hybridization cDNA library was constructed. A total of 454 cDNA clones were identified to be viral infection-induced up-regulated genes. The influence of Rop1 on the infection of maize by SCMV was investigated. The results showed that transient silencing of the ZmRop1 gene through virus-induced gene silencing enhanced the accumulation and systemic infection of SCMV and another potyvirus (Pennisetum mosaic virus) in maize plants, whereas transient over-expression of ZmRop1 in maize protoplasts reduced SCMV accumulation. Furthermore, it was demonstrated that the heterologous expression of ZmRop1 impaired Potato virus X infection in Nicotiana benthamiana plants. These data suggest that ZmRop1 may play a role in plant defence responses to viral infection.
authors.affiliation: State Key Laboratory of Agro-biotechnology and Department of Plant Pathology, China Agricultural University, Beijing 100193, China.
authors.forename: Yanyong
authors.initials: Y
authors.lastname: Cao
authors.forename: Yan
authors.initials: Y
authors.lastname: Shi
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Li
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Cheng
authors.forename: Tao
authors.initials: T
authors.lastname: Zhou
authors.forename: Zaifeng
authors.initials: Z
authors.lastname: Fan
mesh_terms: D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D017343:Genes, Plant / Q000235:genetics; D009029:Mosaic Viruses / Q000502:physiology; D009693:Nucleic Acid Hybridization; D010935:Plant Diseases / Q000821:virology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D010942:Plant Viruses / Q000502:physiology*; D017863:Potexvirus / Q000502:physiology; D011523:Protoplasts / Q000378:metabolism / Q000821:virology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014026:Nicotiana / Q000821:virology; D015854:Up-Regulation / Q000235:genetics; D003313:Zea mays / Q000235:genetics / Q000276:immunology* / Q000821:virology*
chemical_list: D010940:Plant Proteins; D012333:RNA, Messenger