title: Interaction of plectin and intermediate filaments.
abstract: BACKGROUND
  Plectin, a member of the plakin family proteins, is a high molecular weight protein that is ubiquitously expressed. It acts as a cytolinker for the three major components of the cyotoskeleton, namely actin microfilaments, microtubules and intermediate filaments.
  
  
  OBJECTIVE
  The aim of our experiments was to identify new binding sites for intermediate filaments on plectin and to specify these sites.
  
  
  METHODS
  We introduced truncated forms of plectin into several cell lines and observe interaction between plectin and intermediate filaments.
  
  
  RESULTS
  We found that a linker region in the COOH-terminal end of plectin was required for the association of the protein with intermediate filaments. In addition, we also demonstrated that a serine residue at position 4645 of plectin may have a role on binding of plectin to intermediate filaments.
  
  
  CONCLUSIONS
  A linker region in the COOH-terminal end and serine residue at position 4645 may be important for the binding of plectin to intermediate filaments.
authors.affiliation: Department of Dermatology, School of Medicine, and Institute of Cutaneous Cell Biology, Kurume University, 67 Asahimachi, Kurume, Fukuoka, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Karashima
authors.forename: Daisuke
authors.initials: D
authors.lastname: Tsuruta
authors.forename: Takahiro
authors.initials: T
authors.lastname: Hamada
authors.forename: Norito
authors.initials: N
authors.lastname: Ishii
authors.forename: Fumitake
authors.initials: F
authors.lastname: Ono
authors.forename: Keiko
authors.initials: K
authors.lastname: Hashikawa
authors.forename: Bungo
authors.initials: B
authors.lastname: Ohyama
authors.forename: Yohei
authors.initials: Y
authors.lastname: Natsuaki
authors.forename: Shunpei
authors.initials: S
authors.lastname: Fukuda
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Koga
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Sogame
authors.forename: Takekuni
authors.initials: T
authors.lastname: Nakama
authors.forename: Teruki
authors.initials: T
authors.lastname: Dainichi
authors.forename: Takashi
authors.initials: T
authors.lastname: Hashimoto
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism; D000230:Adenocarcinoma; D000310:Adrenal Gland Neoplasms; D000818:Animals; D019556:COS Cells; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D003432:Cross-Linking Reagents / Q000378:metabolism; D017353:Gene Deletion; D007382:Intermediate Filaments / Q000378:metabolism*; D015603:Keratinocytes / Q000166:cytology / Q000502:physiology; D007633:Keratins / Q000378:metabolism; D051190:Plectin / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding / Q000502:physiology; D017434:Protein Structure, Tertiary; D014746:Vimentin / Q000235:genetics / Q000378:metabolism
chemical_list: D003432:Cross-Linking Reagents; C495332:PLEC protein, human; D051190:Plectin; D014746:Vimentin; D007633:Keratins