title: Expression of exogenous human hepatic nuclear factor-1α by a lentiviral vector and its interactions with Plasmodium falciparum subtilisin-like protease 2.
abstract: The onset, severity, and ultimate outcome of malaria infection are influenced by parasite-expressed virulence factors as well as by individual host responses to these determinants. In both humans and mice, liver injury follows parasite entry, persisting to the erythrocytic stage in the case of infection with the fatal strain of Plasmodium falciparum. Hepatic nuclear factor (HNF)-1α is a master regulator of not only the liver damage and adaptive responses but also diverse metabolic functions. In this study, we analyzed the expression of host HNF-1α in relation to malaria infection and evaluated its interaction with the 5'-untranslated region of subtilisin-like protease 2 (subtilase, Sub2). Recombinant human HNF-1α expressed by a lentiviral vector (LV HNF-1α) was introduced into mice. Interestingly, differences in the activity of the 5'-untranslated region of the Pf-Sub2 promoter were detected in 293T cells, and LV HNF-1α was observed to influence promoter activity, suggesting that host HNF-1α interacts with the Sub2 gene.
authors.affiliation: Department of Infection Biology, Zoonosis Research Center, Wonkwang University School of Medicine, Iksan 570-749, Korea.
authors.forename: Shunyao
authors.initials: S
authors.lastname: Liao
authors.forename: Yunqiang
authors.initials: Y
authors.lastname: Liu
authors.forename: Bing
authors.initials: B
authors.lastname: Zheng
authors.forename: Pyo Yun
authors.initials: PY
authors.lastname: Cho
authors.forename: Hyun Ok
authors.initials: HO
authors.lastname: Song
authors.forename: Yun-Seok
authors.initials: YS
authors.lastname: Lee
authors.forename: Suk-Yul
authors.initials: SY
authors.lastname: Jung
authors.forename: Hyun
authors.initials: H
authors.lastname: Park
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics*; D000818:Animals; D002460:Cell Line; D016054:DNA, Protozoan / Q000235:genetics; D005786:Gene Expression Regulation / Q000235:genetics*; D005822:Genetic Vectors; D051538:Hepatocyte Nuclear Factor 1-alpha / Q000008:administration & dosage / Q000235:genetics / Q000378:metabolism*; D006790:Host-Parasite Interactions; D006801:Humans; D007275:Injections, Intravenous; D016086:Lentivirus / Q000235:genetics; D016778:Malaria, Falciparum / Q000378:metabolism / Q000469:parasitology* / Q000473:pathology; D051379:Mice; D010963:Plasmodium falciparum / Q000187:drug effects / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D016053:RNA, Protozoan / Q000235:genetics; D011994:Recombinant Proteins; D015398:Signal Transduction; D013381:Subtilisins / Q000235:genetics* / Q000378:metabolism
chemical_list: D020121:5' Untranslated Regions; D016054:DNA, Protozoan; C496487:HNF1A protein, human; D051538:Hepatocyte Nuclear Factor 1-alpha; D012333:RNA, Messenger; D016053:RNA, Protozoan; D011994:Recombinant Proteins; D013381:Subtilisins; C119550:sub-2 protein, Plasmodium falciparum