title: Molecular evolution of hydrogen peroxide degrading enzymes.
abstract: For efficient removal of intra- and/or extracellular hydrogen peroxide by dismutation to harmless dioxygen and water (2H(2)O(2) → O(2) + 2H(2)O), nature designed three metalloenzyme families that differ in oligomeric organization, monomer architecture as well as active site geometry and catalytic residues. Here we report on the updated reconstruction of the molecular phylogeny of these three gene families. Ubiquitous typical (monofunctional) heme catalases are found in all domains of life showing a high structural conservation. Their evolution was directed from large subunit towards small subunit proteins and further to fused proteins where the catalase fold was retained but lost its original functionality. Bifunctional catalase-peroxidases were at the origin of one of the two main heme peroxidase superfamilies (i.e. peroxidase-catalase superfamily) and constitute a protein family predominantly present among eubacteria and archaea, but two evolutionary branches are also found in the eukaryotic world. Non-heme manganese catalases are a relatively small protein family with very old roots only present among bacteria and archaea. Phylogenetic analyses of the three protein families reveal features typical (i) for the evolution of whole genomes as well as (ii) for specific evolutionary events including horizontal gene transfer, paralog formation and gene fusion. As catalases have reached a striking diversity among prokaryotic and eukaryotic pathogens, understanding their phylogenetic and molecular relationship and function will contribute to drug design for prevention of diseases of humans, animals and plants.
authors.affiliation: Division of Biochemistry, Department of Chemistry, Vienna Institute of BioTechnology at BOKU - University of Natural Resources and Life Sciences, Muthgasse 18, A-1190 Vienna, Austria. marcel.zamocky@boku.ac.at
authors.forename: Marcel
authors.initials: M
authors.lastname: Zámocký
authors.forename: Bernhard
authors.initials: B
authors.lastname: Gasselhuber
authors.forename: Paul G
authors.initials: PG
authors.lastname: Furtmüller
authors.forename: Christian
authors.initials: C
authors.lastname: Obinger
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D002374:Catalase / Q000737:chemistry / Q000378:metabolism; D020134:Catalytic Domain; D029968:Escherichia coli Proteins / Q000378:metabolism; D019143:Evolution, Molecular; D018014:Gene Transfer Techniques; D006418:Heme / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry*; D008345:Manganese / Q000737:chemistry; D008956:Models, Chemical; D009195:Peroxidase / Q000737:chemistry; D010802:Phylogeny; D011506:Proteins / Q000737:chemistry; D017422:Sequence Analysis, DNA
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D011506:Proteins; D006418:Heme; D008345:Manganese; D006861:Hydrogen Peroxide; D002374:Catalase; C051253:katG protein, E coli; D009195:Peroxidase