title: Folic acid-decorated nanocomposites prepared by a simple solvent displacement method.
abstract: A biodegradable nanocarrier system based on PLGA applicable for FR targeting is described. PEI-based conjugates with covalently coupled folic acid are synthesized, characterized with regard to their composition and used for DNA complexation. The preparation of composites is performed by a solvent displacement technique, assuming an electrostatic interaction of PEI-based polyplexes with PLGA. The synthesis of a folic acid-PEG3kDa-PEI25kDa conjugate is achieved. Blending of PLGA with polyplexes results in spherical nanoparticles with sizes ≤ 250 nm. Incorporation of polyplexes and the localization of folic acid on the particle surface, performed by antibody binding, is confirmed. The method is suitable for the preparation of nanosized, folic-acid-decorated nanoparticles.
authors.affiliation: Department of Pharmaceutics and Biopharmacy, Philipps-Universität Marburg, Ketzerbach 63, 35037 Marburg, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Benfer
authors.forename: Regina
authors.initials: R
authors.lastname: Reul
authors.forename: Thomas
authors.initials: T
authors.lastname: Betz
authors.forename: Thomas
authors.initials: T
authors.lastname: Kissel
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry; D001672:Biocompatible Materials / Q000138:chemical synthesis*; D004247:DNA / Q000737:chemistry; D004337:Drug Carriers / Q000138:chemical synthesis*; D005399:Fishes; D005492:Folic Acid / Q000737:chemistry*; D006801:Humans; D007097:Imines / Q000737:chemistry; D019344:Lactic Acid / Q000737:chemistry; D018625:Microscopy, Atomic Force; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry; D011095:Polyethylenes / Q000737:chemistry; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D012997:Solvents / Q000737:chemistry; D055672:Static Electricity
chemical_list: D000906:Antibodies; D001672:Biocompatible Materials; D004337:Drug Carriers; D007097:Imines; D011095:Polyethylenes; D012997:Solvents; C505405:poly(ethylene imine); D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D011092:Polyethylene Glycols; D004247:DNA; D005492:Folic Acid