title: [Effects of the beta2-adrenergic receptor Gln27Glu variation on changes of serum lipid and apolipoprotein ratios induced by a high-carbohydrate/low-fat diet in healthy youth].
abstract: OBJECTIVE
  To investigate the effects of the Gln27Glu polymorphism of beta2-adrenergic receptor (beta2AR) on serum lipid and apolipoprotein ratios and its interaction with high-carbohydrate/low-fat (HC/LF) diet on the ratios in healthy youth.
  
  
  METHODS
  After on a washout diet for seven days, fifty six healthy young subjects were given the HC/LF diet for six days. The 12 hour-fasting serum lipids and apolipoproteins (apo) AI and B100 were measured on the 1st, the 8th and the 14th days. The ratios of TG/HDL-C, log (TG/HDL-C), TC/HDL-C, LDL-C/HDL-C and apoAI/apoB100 were calculated. The polymorphism of Gln27Glu was analyzed by PCR-RFLP method.
  
  
  RESULTS
  No significant differences were found of the lipid and apolipoprotein ratios at baseline and before the HC/LF diet between the subjects with the CC genotype (wide type) and the G carriers (mutation carriers) in the whole study sample or the males and the females separately. The G carriers had a significantly higher level of log (TG/HDL-C) (P=0.038) than the subjects with the CC genotype did after the HC/LF diet in the whole study sample but not in the males and the females separately. Significant decreases of LDL-C/HDL-C and TC/HDL-C were observed in all the subjects after the HC/LF diet when compared with those before the HC/LF diet (P<0.05), but a significant increase of apoAI/apoB100 (P=0.021) only in the subjects with the CC genotype. When gender was taken into account, significant decreases of LDL-C/HDL-C and TC/HDL-C were found after the HC/ LF diet in the male subjects regardless of genotypes (P<0.05). Significant increases of TG/HDL-C and log (TG/ HDL-C) and a significant decrease of TC/HDL-C were found in all the female subjects (P<0.05), while a significant decrease of LDL-C/HDL-C (P=0.037) was only observed in the female subjects with the CC genotype.
  
  
  CONCLUSIONS
  The G allele of beta2AR Gln27Glu variation can inhibit the decrease of LDL-C/HDL-C in females after HC/LF diet intervention.
authors.affiliation: Department of Biochemistry and Molecular Biology Laboratory, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Guo-jin
authors.initials: GJ
authors.lastname: Ou
authors.forename: Ding-zhi
authors.initials: DZ
authors.lastname: Fang
authors.forename: Ren-rong
authors.initials: RR
authors.lastname: Gong
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
authors.forename: Yuan-hao
authors.initials: YH
authors.lastname: Li
authors.forename: Zhe
authors.initials: Z
authors.lastname: Jiang
authors.forename: Mei
authors.initials: M
authors.lastname: Fan
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D001053:Apolipoproteins / Q000097:blood*; D018752:Diet, Fat-Restricted; D004040:Dietary Carbohydrates / Q000008:administration & dosage*; D005260:Female; D005838:Genotype; D018698:Glutamic Acid / Q000235:genetics / Q000378:metabolism; D005973:Glutamine / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008055:Lipids / Q000097:blood*; D008297:Male; D009154:Mutation / Q000235:genetics / Q000502:physiology; D011110:Polymorphism, Genetic / Q000235:genetics; D018343:Receptors, Adrenergic, beta-2 / Q000235:genetics* / Q000378:metabolism; D055815:Young Adult
chemical_list: D001053:Apolipoproteins; D004040:Dietary Carbohydrates; D008055:Lipids; D018343:Receptors, Adrenergic, beta-2; D005973:Glutamine; D018698:Glutamic Acid