title: Bovine serum albumin film as a template for controlled nanopancake and nanobubble formation: in situ atomic force microscopy and nanolithography study.
abstract: Air nanobubbles and nanopancakes were investigated in situ by both tapping mode atomic force microscopy (TM AFM) and atomic force nanolithography techniques employing bovine serum albumin (BSA) film supported by highly oriented pyrolytic graphite (HOPG). The BSA denaturation induced by the water-to-ethanol exchange served for conservation of nanobubble and nanopancake sites appearing as imprints in BSA film left by gaseous cavities formerly present on the interface in the aqueous environment. Once the BSA film was gently removed by the nanoshaving technique applied in ethanol, a clean basal plane HOPG area with well-defined dimensions was regenerated. The subsequent reverse ethanol-to-water exchange led to the re-formation of nanopancakes specifically at the nanoshaved area. Our approach paves the way for the study of gaseous nanostructures with defined dimensions, formed at solid-liquid interface under controlled conditions.
authors.affiliation: J. Heyrovský Institute of Physical Chemistry of ASCR, vvi, Prague, Czech Republic. viliam.kolivoska@jh-inst.cas.cz
authors.forename: Viliam
authors.initials: V
authors.lastname: Kolivoška
authors.forename: Miroslav
authors.initials: M
authors.lastname: Gál
authors.forename: Magdaléna
authors.initials: M
authors.lastname: Hromadová
authors.forename: Stěpánka
authors.initials: S
authors.lastname: Lachmanová
authors.forename: Hana
authors.initials: H
authors.lastname: Tarábková
authors.forename: Pavel
authors.initials: P
authors.lastname: Janda
authors.forename: Lubomír
authors.initials: L
authors.lastname: Pospíšil
authors.forename: Andrea Morovská
authors.initials: AM
authors.lastname: Turoňová
mesh_terms: D000388:Air; D000818:Animals; D002417:Cattle; D000431:Ethanol; D005740:Gases; D006108:Graphite / Q000737:chemistry; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013499:Surface Properties; D014867:Water
chemical_list: D005740:Gases; D014867:Water; D012710:Serum Albumin, Bovine; D000431:Ethanol; D006108:Graphite