title: DNA array with the groESL intergenic sequence to detect Vibrio parahaemolyticus and Vibrio vulnificus.
abstract: The untranscribed DNA sequences of the intergenic spacer regions (ISRs) in the groESL gene of 23 Vibrio species were determined and compared. ISR sequence length (41-85 bp) was variable. Vibrio species could be divided into three groups according to the length and homology of their ISR sequences. DNA array hybridization using ISR-specific probes accurately distinguished Vibrio parahaemolyticus and Vibrio vulnificus from other species.
authors.affiliation: Department of Biotechnology, Pukyong National University, Busan 608-737, Republic of Korea.
authors.forename: Yu-Ri
authors.initials: YR
authors.lastname: Kim
authors.forename: Eun-Young
authors.initials: EY
authors.lastname: Kim
authors.forename: Dong-Gyun
authors.initials: DG
authors.lastname: Kim
authors.forename: Young-Ok
authors.initials: YO
authors.lastname: Kim
authors.forename: Muhammad Tofazzal
authors.initials: MT
authors.lastname: Hossain
authors.forename: In-Soo
authors.initials: IS
authors.lastname: Kong
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D018833:Chaperonins / Q000235:genetics*; D021901:DNA, Intergenic / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016415:Sequence Alignment; D013698:Templates, Genetic; D014736:Vibrio parahaemolyticus / Q000235:genetics* / Q000302:isolation & purification*; D041261:Vibrio vulnificus / Q000235:genetics* / Q000302:isolation & purification*
chemical_list: D001426:Bacterial Proteins; D021901:DNA, Intergenic; C097970:GroESL protein, Bacteria; D018833:Chaperonins