title: [Influence of epidermal growth factor receptor monoclony antibody MAb225 on DNA repair of tongue carcinoma cell after radiation].
abstract: OBJECTIVE
  The objective of this study is to investigate the effect of epidermal growth factor receptor (EGFR) monoclonal antibody MAb225 on repair of DNA double strand break (DNA-DSB) after radiation in tongue squamous cell carcinoma cell.
  
  
  METHODS
  The single cell gel electrophoresis (SCGE) was performed to estimate the repair of DNA-DSB induced by radiation in human tongue carcinoma cells Tca8113 treated with or without MAb225. Expression of Ku70 and Ku80 were detected by semiquantitative reverse transcription polymerase chain reaction and Western bolt.
  
  
  RESULTS
  Comet tail moment of MAb225 treated cell was significantly higher than untreated cell (P < 0.05). The expression of Ku70 and Ku80 were inhibited by MAb225.
  
  
  CONCLUSIONS
  MAb225 can inhibit repair of DNA-DSB induced by down-regulated expression of Ku70 and Ku80.
authors.affiliation: Dept. of Stomatology, The Affiliated Jinan Central Hospital of Shandong University, Jinan 250013, China.
authors.forename: Mingguo
authors.initials: M
authors.lastname: Wang
authors.forename: Zhonghe
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000911:Antibodies, Monoclonal; D034961:Antigens, Nuclear; D004260:DNA Repair; D004268:DNA-Binding Proteins; D066246:ErbB Receptors; D006801:Humans; D000072200:Ku Autoantigen; D014062:Tongue Neoplasms
chemical_list: D000911:Antibodies, Monoclonal; D034961:Antigens, Nuclear; D004268:DNA-Binding Proteins; D066246:ErbB Receptors; C000606509:Xrcc6 protein, human; D000072200:Ku Autoantigen