title: The histobiochemical effects of melatonin on ischemia reperfusion-related injuries in vascular trauma of lower limbs.
abstract: BACKGROUND
  The aim of this study was to evaluate the protective effect of melatonin on ischemia-reperfusion syndrome.
  
  
  METHODS
  Thirty-three adult male Wistar albino rats were randomized into three experimental groups of 11: Group C --control group with no ischemia or reperfusion. Groups I/R and I/R + M had 2.5 hours of ischemia and of two hours of reperfusion by means of clamping of the common femoral artery. All the animals received maintenance fluid with intraperitoneal (i.p) normal saline following general anesthesia. The animals of Group I/R + M were treated with i.p Melatonin (10 mg/kg) five minutes before reperfusion. At the end of reperfusion, samples were taken for histological evaluation and biochemical analysis. Parameters studied were biopsies of the soleus muscle, level of lactate, creatine phosphokinase (CPK), lactate dehydrogenase (LDH), sodium, potassium (K), calcium (Ca) and arterial blood gasometry.
  
  
  RESULTS
  In I/R group, the levels of K, CPK increased dramatically contrast with groups C and IR+M (P < 0.05). A significant decrease in HCO3 was found in I/R Group in comparison with Group IR+M and Group C (P < 0.001). In Group IR+M, lactate level decreased dramatically compared to other groups (P < 0.001). Histological injury in I/R + M was found to be less than in I/R group (P < 0.05). There was no significant difference in PO2, pH, carbon dioxide, partial pressure of oxygen, Na, LDH, Ca and P in three groups (P > 0.05). Histological change in the group C and group M didn't differ significantly, but the difference in group I/R was significant compared to group C and IR+M (P < 0.05).
  
  
  CONCLUSIONS
  We suggest that melatonin has protective effect against I/R syndrome in blood and skeletal muscle and may reduce the morbidity following revascularization surgery in vascular trauma.
authors.affiliation: Department of Surgery, Isfahan University of Medical Sciences Gonabad University of Medical Sciences, Isfahan Gonabad, Iran.
authors.forename: Rohollah
authors.initials: R
authors.lastname: Sobhani
authors.forename: Hassan
authors.initials: H
authors.lastname: Masoudpour
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Akbari
authors.forename: Hamid Reza
authors.initials: HR
authors.lastname: Suzangar
authors.forename: Samira
authors.initials: S
authors.lastname: AleSaeidio
authors.forename: Shahriar
authors.initials: S
authors.lastname: Adibi
authors.forename: Sayed Amir Hossein
authors.initials: SA
authors.lastname: Khademi
authors.forename: Elham Fatemeh
authors.initials: EF
authors.lastname: Khademi
authors.forename: Fahimeh
authors.initials: F
authors.lastname: Sobhani
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D004195:Disease Models, Animal; D035002:Lower Extremity / Q000473:pathology* / Q000503:physiopathology; D008297:Male; D008550:Melatonin / Q000494:pharmacology*; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000473:pathology* / Q000503:physiopathology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000473:pathology* / Q000503:physiopathology; D016896:Treatment Outcome
chemical_list: D000975:Antioxidants; D008550:Melatonin