title: Investigation into the dosage of dietary sphingomyelin concentrate in relation to the improvement of epidermal function in hairless mice.
abstract: We previously found that dietary sphingomyelin (SPM) concentrate from bovine milk improved epidermal function. In this study, we investigated the dosage of dietary SPM concentrate from bovine milk in relation to the improvement of epidermal function. Thirteen-week-old hairless male mice were separated into four experimental groups, each fed one of four types of experimental diet: the control group, the low SPM group, the medium SPM group and the high SPM group. The mice were each fed the experimental diet for 6 weeks. The stratum corneum hydration and the transepidermal water loss (TEWL) were measured using a Corneometer and a Tewameter at 3 weeks and 6 weeks. After the feeding period, ceramides in the stratum corneum were analyzed. We found that the stratum corneum hydration in all the SPM groups was significantly higher than that in the control group, whereas TEWL in all the SPM groups was significantly lower than that in the control group. Ceramides increased significantly in mice fed the medium SPM diet and statistically tended to increase in mice fed the high SPM diet. Our results indicate that a daily intake of 17 mg SPM concentrate is enough to improve epidermal function in hairless mice.
authors.affiliation: Milk Science Research Institute, Megmilk Snow Brand Co., Ltd, Kawagoe, Saitama, Japan. y-haruta@meg-snow.com
authors.forename: Yuko
authors.initials: Y
authors.lastname: Haruta-Ono
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ueno
authors.forename: Noriko
authors.initials: N
authors.lastname: Ueda
authors.forename: Ken
authors.initials: K
authors.lastname: Kato
authors.forename: Toshimitsu
authors.initials: T
authors.lastname: Yoshioka
mesh_terms: D000818:Animals; D001834:Body Water / Q000378:metabolism; D001835:Body Weight; D002518:Ceramides / Q000378:metabolism; D019587:Dietary Supplements; D004305:Dose-Response Relationship, Drug; D004435:Eating; D004817:Epidermis / Q000378:metabolism / Q000502:physiology*; D008297:Male; D051379:Mice; D008812:Mice, Hairless / Q000378:metabolism / Q000502:physiology*; D008892:Milk / Q000737:chemistry; D013109:Sphingomyelins / Q000008:administration & dosage* / Q000032:analysis; D013997:Time Factors
chemical_list: D002518:Ceramides; D013109:Sphingomyelins