title: Enhanced mechanical properties of rabbit flexor tendons in response to intratendinous injection of adipose derived stromal vascular fraction.
abstract: BACKGROUND
  Tendon injuries are notorious for slow and functionally inferior healing. It is claimed that cell-based therapy would result in faster and more efficient healing of injured tissues with less postoperative complications. Given the limitations associated with ex vivo cellular expansion, we tried to evaluate the possible effects of intratendinous injection of adipose derived stromal vascular fraction on mechanical properties of tendon repair.
  
  
  METHODS
  The model of injury was complete sharp transection of rabbit deep digital flexor tendon followed by primary suture repair and an intratendinous injection of either allogeneic stromal vascular fraction or placebo. Tendons were harvested at three and eight weeks after surgery.
  
  
  RESULTS
  The results of mechanical testing showed the treatment caused significant increase in ultimate and yield loads, stress, and energy absorption of repairs compared to controls at both time points. Also, improvement in terms of strain and stiffness were detected at the eighth week in treatments.
  
  
  CONCLUSIONS
  In comparison with the result of previous studies using cultured mesenchymal stem cells from bone marrow or adipose tissue; the improved mechanical properties observed in the present study suggest that choosing stromal vascular fraction as a readily accessible and instant source of multipotent cells instead of expensive and long-lasting culture expansion may seem more favorable in cell based therapy for tendon injuries. The mechanical functionality of the repairs observed in the present study encourages further investigations into the use of stromal vascular fraction for the repair of tendon injuries.
authors.affiliation: Department of Clinical Sciences, Urmia University, Nazloo Road, Urmia, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Behfar
authors.forename: Farshid
authors.initials: F
authors.lastname: Sarrafzadeh-Rezaei
authors.forename: Rahim
authors.initials: R
authors.lastname: Hobbenaghi
authors.forename: Nowruz
authors.initials: N
authors.lastname: Delirezh
authors.forename: Bahram
authors.initials: B
authors.lastname: Dalir-Naghadeh
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002469:Cell Separation; D004730:Endothelium, Vascular / Q000166:cytology; D007267:Injections; D008297:Male; D011817:Rabbits; D020127:Recovery of Function; D017154:Stromal Cells / Q000166:cytology / Q000637:transplantation; D013537:Sutures; D013708:Tendon Injuries / Q000503:physiopathology / Q000628:therapy*; D013710:Tendons / Q000503:physiopathology*; D013718:Tensile Strength; D014945:Wound Healing