title: Effect of amiodarone on thermotolerance and Hsp104p synthesis in the yeast Saccharomyces cerevisiae.
abstract: Amiodarone (AMD) is known to induce a transient increase in cytosolic Ca2+ level in cells of the yeast Saccharomyces cerevisiae. In the present study the effect of AMD on the thermotolerance and Hsp104p synthesis of the yeast was studied. AMD induced Hsp104p synthesis and increased survival of the yeast after a severe heat shock (50°C). The development of thermotolerance to a considerable extent depended on the presence of Hsp104p. The same effect was achieved by treatment with the classical uncoupler CCCP, which is also known to increase the cytosolic Ca2+ level. It is supposed that the change in intracellular Ca2+ concentration plays an important role in activation of the HSP104 gene expression and in increasing the thermotolerance of the yeast. The possible link between mitochondrial activity and calcium homeostasis is discussed.
authors.affiliation: Siberian Institute of Plant Physiology and Biochemistry, Siberian Division of the Russian Academy of Sciences, Irkutsk, Russia. fedoseeva@sifibr.irk.ru
authors.forename: I V
authors.initials: IV
authors.lastname: Fedoseeva
authors.forename: D V
authors.initials: DV
authors.lastname: Pjatricas
authors.forename: N N
authors.initials: NN
authors.lastname: Varakina
authors.forename: T M
authors.initials: TM
authors.lastname: Rusaleva
authors.forename: A V
authors.initials: AV
authors.lastname: Stepanov
authors.forename: E G
authors.initials: EG
authors.lastname: Rikhvanov
authors.forename: G B
authors.initials: GB
authors.lastname: Borovskii
authors.forename: V K
authors.initials: VK
authors.lastname: Voinikov
mesh_terms: D000638:Amiodarone / Q000737:chemistry / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D006360:Heat-Shock Proteins / Q000378:metabolism*; D010766:Phosphorylation; D012235:Rhodamines / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D013696:Temperature
chemical_list: D004791:Enzyme Inhibitors; D006360:Heat-Shock Proteins; D012235:Rhodamines; D029701:Saccharomyces cerevisiae Proteins; C401833:tetramethylrhodamine methyl ester; C070972:HsP104 protein, S cerevisiae; D000638:Amiodarone; D002118:Calcium