title: Death rate in a small air-lift loop reactor of vero cells grown on solid microcarriers and in macroporous microcarriers.
abstract: The death rate of Vero cells grown on Cytodex-3 microcarrierswas studied as a function of the gas flow rate in a smallair-lift loop reactor. The death rate may be described byfirst-order death-rate kinetics. The first-order death-rateconstant as calculated from the decrease in viable cells, theincrease in dead cells and the increase in LDH activity islinear proportional to the gas flow rate, with a specifichypothetical killing volume in which all cells are killed ofabout 2.10(-3)m(3) liquid per m(3) of air bubbles.In addition, an experiment was conducted in the sameair-lift reactor with Vero cells grown inside porous Asahimicrocarriers. The specific hypothetical killing volumecalculated from this experiment has a value of 3.10(-4)m(3) liquid per m(3) of air bubbles, which shows thatthe porous microcarriers were at least in part able to protectthe cells against the detrimental hydrodynamic forcesgenerated by the bubbles.
authors.affiliation: Department of Food Science, Food and Bioengeneering Group, Agricultural University, Bomenweg 2, 6703 HD, Wageningen, The Netherlands.
authors.forename: D E
authors.initials: DE
authors.lastname: Martens
authors.forename: E A
authors.initials: EA
authors.lastname: Nollen
authors.forename: M
authors.initials: M
authors.lastname: Hardeveld
authors.forename: C A
authors.initials: CA
authors.lastname: Velden-de Groot
authors.forename: C D
authors.initials: CD
authors.lastname: Gooijer
authors.forename: E C
authors.initials: EC
authors.lastname: Beuvery
authors.forename: J
authors.initials: J
authors.lastname: Tramper