title: Plasma membrane-associated glycohydrolases along differentiation of murine neural stem cells.
abstract: The activities of plasma membrane associated sialidase Neu3, total β-glucosidase, CBE-sensitive β-glucosidase, non-lysosomal β-glucosyl ceramidase GBA2, β-galactosidase, β-hexosaminidase and sphingomyelinase were determined at three different stages of differentiation of murine neural stem cell cultures, corresponding to precursors, commited progenitors, and differentiated cells. Cell immunostaining for specific markers of the differentiation process, performed after 7 days in culture in presence of differentiating agents, clearly showed the presence of oligodendrocytes, astrocytes and neurons. Glial cells were the most abundant. Sialidase Neu3 after a decrease from progenitors to precursors, showed an increase parallel to the differentiation process. All the other glycosidases increased their activity along differentiation. The activity of CBE-sensitive β-glucosidase and GBA2 were very similar at the precursor stage, but CBE-sensitive β-glucosidase increased 7 times while GBA2 only two in the differentiated cells. In addition, we analysed also sphingomyelinase as enzyme specifically associated to sphingolipids. The activity of this enzyme increased from precursors to differentiated cells.
authors.affiliation: Department of Medical Chemistry, Biochemistry and Biotechnology, University of Milan, Via Fratelli Cervi 93, 20090 Segrate, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Aureli
authors.forename: Angela
authors.initials: A
authors.lastname: Gritti
authors.forename: Rosaria
authors.initials: R
authors.lastname: Bassi
authors.forename: Nicoletta
authors.initials: N
authors.lastname: Loberto
authors.forename: Alessandra
authors.initials: A
authors.lastname: Ricca
authors.forename: Vanna
authors.initials: V
authors.lastname: Chigorno
authors.forename: Alessandro
authors.initials: A
authors.lastname: Prinetti
authors.forename: Sandro
authors.initials: S
authors.lastname: Sonnino
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000201:enzymology*; D002454:Cell Differentiation / Q000502:physiology*; D002462:Cell Membrane / Q000201:enzymology*; D005962:Glucosylceramidase / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism*; D051379:Mice; D058953:Neural Stem Cells / Q000201:enzymology*; D009439:Neuraminidase / Q000378:metabolism; D009474:Neurons / Q000201:enzymology*; D009836:Oligodendroglia / Q000201:enzymology*; D013108:Sphingomyelin Phosphodiesterase / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism; D001617:beta-Glucosidase / Q000378:metabolism; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
chemical_list: D013108:Sphingomyelin Phosphodiesterase; D006026:Glycoside Hydrolases; C511796:Neu3 protein, mouse; D009439:Neuraminidase; D001617:beta-Glucosidase; C517538:beta-glucosidase 2, mouse; D001616:beta-Galactosidase; D005962:Glucosylceramidase; D001619:beta-N-Acetylhexosaminidases