title: Actinoplanes rishiriensis sp. nov., a novel motile actinomycete isolated by rehydration and centrifugation method.
abstract: The motile actinomycete strain RI50-RCA114(T) was isolated using rehydration and centrifugation method from a soil sample obtained from Rishiri Island in Japan. The taxonomic status of this organism was established using a polyphasic approach. Cells of strain RI50-RCA114(T) were Gram positive, aerobic, motile and formed irregular sporangia. The strain grew in the presence of 0-2% (w/v) NaCl, between pH 5 and 8, and over a temperature range of 20-37°C, with optimal growth at 30°C. Whole-cell hydrolysates of the strain contained meso-diaminopimelic acid, galactose, glucose and mannose, in addition to one unidentified O-methyl-hexose. The predominant menaquinone was MK-9(H(4)), and the major polar lipids comprised phosphatidylethanolamine, diphosphatidylglycerol and phosphatidyl-N-methylethylethanolamine. The major cellular fatty acids were iso-C(16:0), iso-C(15:0) and anteiso-C(17:0). Comparative 16S ribosomal RNA gene sequence analysis revealed that strain RI50-RCA114(T) had the closest sequence similarity with Actinoplanes globisporus JCM 3186(T) (97.6%). However, DNA-DNA hybridization assays as well as physiological and biochemical analyses differentiated strain RI50-RCA114(T) from its closest phylogenetic relative. On the basis of these data, we propose that strain RI50-RCA114(T) (=NBRC 108556(T)=BCC 49184(T)) be classified as the type strain of a novel Actinoplanes species and named Actinoplanes rishiriensis sp. nov.
authors.affiliation: Division of Applied Biological Sciences, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Kofu, Japan. hyamamura@yamanashi.ac.jp
authors.forename: Hideki
authors.initials: H
authors.lastname: Yamamura
authors.forename: Aya
authors.initials: A
authors.lastname: Shimizu
authors.forename: Youji
authors.initials: Y
authors.lastname: Nakagawa
authors.forename: Moriyuki
authors.initials: M
authors.lastname: Hamada
authors.forename: Misa
authors.initials: M
authors.lastname: Otoguro
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Tamura
authors.forename: Masayuki
authors.initials: M
authors.lastname: Hayakawa
mesh_terms: D039903:Actinobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D007564:Japan; D009693:Nucleic Acid Hybridization / Q000379:methods; D010802:Phylogeny; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017423:Sequence Analysis, RNA; D012988:Soil Microbiology; D013045:Species Specificity
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S