title: Evidence that the kinesin light chain domain contains tetratricopeptide repeat units.
abstract: Homology models were built for various length sequences of the kinesin-1 light chain (KLC) domain of Drosophila melanogaster and subjected to 200 ns of all-atom molecular dynamics. We also cloned, expressed and characterized these regions spectroscopically. Results confirm that KLC contains tetratricopeptide repeat units; a regular array of repeating 34-residue helix-loop-helix monomers. Experimental and computational evidence is provided confirming the stability and overall helicity of individual TPR repeats as well as individual TPR units incorporated into a multi-TPR structure.
authors.affiliation: Department of Chemistry, Gottwald Center for the Sciences, University of Richmond, Richmond, VA 23173, USA.
authors.forename: Sally Q
authors.initials: SQ
authors.lastname: Fisher
authors.forename: Meredith
authors.initials: M
authors.lastname: Weck
authors.forename: Jenna E
authors.initials: JE
authors.lastname: Landers
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Emrich
authors.forename: Shana A
authors.initials: SA
authors.lastname: Middleton
authors.forename: Jordan
authors.initials: J
authors.lastname: Cox
authors.forename: Lisa
authors.initials: L
authors.lastname: Gentile
authors.forename: Carol A
authors.initials: CA
authors.lastname: Parish
mesh_terms: D000818:Animals; D029721:Drosophila Proteins / Q000737:chemistry*; D004331:Drosophila melanogaster; D016547:Kinesins / Q000737:chemistry*; D008958:Models, Molecular; D009842:Oligopeptides / Q000737:chemistry*; D011487:Protein Conformation
chemical_list: D029721:Drosophila Proteins; D009842:Oligopeptides; D016547:Kinesins