title: Antiviral effect of geraniin on human enterovirus 71 in vitro and in vivo.
abstract: Human enterovirus 71 infection causes hand, foot and mouth disease in children under 6 years of age and has caused mortalities in large-scale outbreaks in the Asia-Pacific region. No effective vaccine or antiviral drugs currently exist against enterovirus 71 in the clinic. In this study, we investigated the antiviral effect of geraniin on enterovirus 71 both in vitro and in vivo. The results showed that geraniin effectively inhibited virus replication in rhabdomyosarcoma cells with an IC(50) of 10 μg/ml. Moreover, geraniin treatment of mice that were challenged with a lethal dose of enterovirus 71 resulted in a reduction of mortality, relieved clinical symptoms, and inhibited virus replication in muscle tissues. The results suggest that geraniin may be used as a potential drug for anti-enterovirus 71.
authors.affiliation: Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health, Institute of Laboratory Animal Science, CAMS & Comparative Medicine Centre, PUMC, Beijing, China.
authors.forename: Yajun
authors.initials: Y
authors.lastname: Yang
authors.forename: Lianfeng
authors.initials: L
authors.lastname: Zhang
authors.forename: Xiaoxu
authors.initials: X
authors.lastname: Fan
authors.forename: Chuan
authors.initials: C
authors.lastname: Qin
authors.forename: Jiangning
authors.initials: J
authors.lastname: Liu
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D045744:Cell Line, Tumor; D029821:Enterovirus A, Human / Q000187:drug effects* / Q000502:physiology; D004769:Enterovirus Infections / Q000188:drug therapy* / Q000401:mortality / Q000821:virology; D005960:Glucosides / Q000008:administration & dosage / Q000627:therapeutic use*; D006801:Humans; D047348:Hydrolyzable Tannins / Q000008:administration & dosage / Q000627:therapeutic use*; D051379:Mice; D008813:Mice, Inbred ICR; D015996:Survival Rate; D019562:Viral Load / Q000187:drug effects*; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D005960:Glucosides; D047348:Hydrolyzable Tannins; C024603:Geraniin