title: Sequence typing confirms that a predominant Listeria monocytogenes clone caused human listeriosis cases and outbreaks in Canada from 1988 to 2010.
abstract: Human listeriosis outbreaks in Canada have been predominantly caused by serotype 1/2a isolates with highly similar pulsed-field gel electrophoresis (PFGE) patterns. Multilocus sequence typing (MLST) and multi-virulence-locus sequence typing (MVLST) each identified a diverse population of Listeria monocytogenes isolates, and within that, both methods had congruent subtypes that substantiated a predominant clone (clonal complex 8; virulence type 59; proposed epidemic clone 5 [ECV]) that has been causing human illness across Canada for more than 2 decades.
authors.affiliation: Department of Food Science, Penn State University, University Park, Pennsylvania, USA. sjk9@psu.edu
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Knabel
authors.forename: Aleisha
authors.initials: A
authors.lastname: Reimer
authors.forename: Bindhu
authors.initials: B
authors.lastname: Verghese
authors.forename: Mei
authors.initials: M
authors.lastname: Lok
authors.forename: Jennifer
authors.initials: J
authors.lastname: Ziegler
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Farber
authors.forename: Franco
authors.initials: F
authors.lastname: Pagotto
authors.forename: Morag
authors.initials: M
authors.lastname: Graham
authors.forename: Celine A
authors.initials: CA
authors.lastname: Nadon
authors.forename: Matthew W
authors.initials: MW
authors.lastname: Gilmour
mesh_terms: D002170:Canada / Q000453:epidemiology; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004196:Disease Outbreaks; D006801:Humans; D008089:Listeria monocytogenes / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D008088:Listeriosis / Q000453:epidemiology* / Q000382:microbiology*; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D058889:Molecular Typing; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial