title: Rapid detection of sacbrood virus (SBV) by one-step reverse transcription loop-mediated isothermal amplification assay.
abstract: BACKGROUND
  Sacbrood virus (SBV) primarily infects honeybee broods, and in order to deal with the problem cost effective detection methods are required.
  
  
  RESULTS
  A one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the rapid identification of SBV. The data demonstrated that, in a simple water bath, SBV RNA could be detected as early as 20 min at 65°C, and a positive amplification reaction was visible to the naked eye due to a color change brought on by the addition of nucleic acid stain SYBR Green.
  
  
  CONCLUSIONS
  The current study presents a method for the rapid and simple detection of SBV by RT-LAMP with high sensitivity and analytic specificity.
authors.affiliation: Chongqing Academy of Animal Science, Chongqing 402460, China.
authors.forename: Jin-Long
authors.initials: JL
authors.lastname: Yang
authors.forename: Rui
authors.initials: R
authors.lastname: Yang
authors.forename: Ke-Fei
authors.initials: KF
authors.lastname: Shen
authors.forename: Xiang-Wei
authors.initials: XW
authors.lastname: Peng
authors.forename: Tao
authors.initials: T
authors.lastname: XIong
authors.forename: Zuo-Hua
authors.initials: ZH
authors.lastname: Liu
mesh_terms: D000818:Animals; D001516:Bees / Q000821:virology*; D015751:Genes, pol; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D010849:Picornaviridae / Q000235:genetics / Q000302:isolation & purification*; D010850:Picornaviridae Infections / Q000662:veterinary / Q000821:virology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity