title: Structure of the cytoplasmic domain of Yersinia pestis YscD, an essential component of the type III secretion system.
abstract: The Yersinia pestis YscD protein is an essential component of the type III secretion system. YscD consists of an N-terminal cytoplasmic domain (residues 1-121), a transmembrane linker (122-142) and a large periplasmic domain (143-419). Both the cytoplasmic and the periplasmic domains are required for the assembly of the type III secretion system. Here, the structure of the YscD cytoplasmic domain solved by SAD phasing is presented. Although the three-dimensional structure is similar to those of forkhead-associated (FHA) domains, comparison with the structures of canonical FHA domains revealed that the cytoplasmic domain of YscD lacks the conserved residues that are required for binding phosphothreonine and is therefore unlikely to function as a true FHA domain.
authors.affiliation: Basic Science Program, SAIC-Frederick Inc., National Cancer Institute at Frederick, Frederick, MD 21702-1201, USA.
authors.forename: George T
authors.initials: GT
authors.lastname: Lountos
authors.forename: Joseph E
authors.initials: JE
authors.lastname: Tropea
authors.forename: David S
authors.initials: DS
authors.lastname: Waugh
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D018360:Crystallography, X-Ray; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D040681:Structural Homology, Protein; D013329:Structure-Activity Relationship; D015010:Yersinia pestis / Q000737:chemistry* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins