title: A biomimetic sensor surface to detect anti-β2-glycoprotein-I antibodies as a marker for antiphospholipid syndrome.
abstract: A biomimetic sensor has been developed, that allows for quantification of autoantibodies related to the antiphospholipid syndrome (APS). Autoantibodies directed against the β(2)-glycoprotein-I (β(2)GP-I) are known as the best markers for diagnosis of APS, however, detection of such antibodies is still a challenge. The epitopes of β(2)GP-I are exposed upon binding to negatively charged membranes. The surface of the sensor chips was therefore modified with such type of membranes, on which β(2)GP-I molecules were subsequently immobilized as recognition elements. Using the label-free method, reflectometric interference spectroscopy, it was possible to quantify anti-β(2)GP-I antibodies and to calibrate the sensor chip in buffer. A mild regeneration procedure allows for many consecutive measurements without stripping off the membrane in between.
authors.affiliation: Institute of Physical and Theoretical Chemistry (IPTC), Eberhard Karls University Tübingen, Auf der Morgenstelle 18, 72076, Tübingen, Germany.
authors.forename: Urs
authors.initials: U
authors.lastname: Hilbig
authors.forename: Oliver
authors.initials: O
authors.lastname: Bleher
authors.forename: Alexander
authors.initials: A
authors.lastname: Le Blanc
authors.forename: Günter
authors.initials: G
authors.lastname: Gauglitz
mesh_terms: D016736:Antiphospholipid Syndrome / Q000175:diagnosis / Q000276:immunology*; D001323:Autoantibodies / Q000032:analysis* / Q000276:immunology; D015374:Biosensing Techniques / Q000379:methods*; D002138:Calibration; D000939:Epitopes / Q000276:immunology; D006801:Humans; D055767:Immobilized Proteins / Q000276:immunology; D008567:Membranes, Artificial; D012680:Sensitivity and Specificity; D013057:Spectrum Analysis / Q000379:methods; D053482:beta 2-Glycoprotein I / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D000939:Epitopes; D055767:Immobilized Proteins; D008567:Membranes, Artificial; D053482:beta 2-Glycoprotein I