title: [Effect of ipriflavone on Wistar rats and their litters].
abstract: OBJECTIVE
  Evaluate the effects of ipriflavone during fetogenesis, since no studies have been conducted to assess its effect during this period.
  
  
  METHODS
  60 pregnant rats were divided randomly into four groups (n=15). G-control (1 mL of distilled water) and three groups treated intragastrically with ipriflavone from the 16th to the 20th post coitus (PC) day: G-300 (300 mg/kg), G-1,500 (1,500 mg/kg) and G-3,000 (3,000 mg/kg). The animals were weighed, anaesthetized intraperitoneally with xylazine and ketamine at doses of 180 mg/kg and 10 mg/kg, respectively, and sacrificed by total exsanguination on the 21st day. A complete blood count was performed and serum cholesterol, triglycerides, AST, ALT, urea, creatinine, and glucose were determined in pregnant rats. After laparotomy, the liver, kidneys, adrenals, spleen and ovaries were removed and weighed; fetuses and placentas were also weighed to obtain the average weight of the litters. Four fetuses (two males and two females) were chosen at random for the determination of the length and weight of brain, liver, kidneys and lungs.
  
  
  METHODS
  ANOVA followed by Dunnett's test. For raw data without normal distribution and homoscedasticity, we used the Kruskal-Wallis test followed by the Mann-Whitney test. Proportions were analyzed by the χ² test (p<0.05).
  
  
  RESULTS
  Triglyceride levels (mg/dL) were: Control-G (138.8±21.8), G-300 (211.2±63.9) G-1,500 (251.5±65.2) G-3,000 (217.7±49.6); p<0.05. The body weight of fetuses (g) was: G-Control (male 3.3±0.3; female 3.1±0.3), G-300 (male 3.4±0.2; female 3.1±0.4), G-1,500 (male 3.5±0.3; female 3.2±0.3), G-3,000 (male 3.4±0.5; female 3.1±0.4).
  
  
  CONCLUSIONS
  Ipriflavone did not cause maternal toxicity, but increased triglyceride levels and reduced hematocrit at higher doses. The body and organ weights of the fetuses did not change with dam treatment. There were no external malformations or fetal deaths.
authors.affiliation: Programa de Pós-graduação em Ciências Biológicas, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brasil.
authors.forename: Pedro Martins
authors.initials: PM
authors.lastname: Bellei
authors.forename: Marcella Martins
authors.initials: MM
authors.lastname: Terra
authors.forename: Vera Maria
authors.initials: VM
authors.lastname: Peters
authors.forename: Martha de Oliveira
authors.initials: Mde O
authors.lastname: Guerra
authors.forename: Amaury Teixeira Leite de
authors.initials: AT
authors.lastname: Andrade
mesh_terms: D000818:Animals; D005260:Female; D005333:Fetus / Q000187:drug effects*; D007529:Isoflavones / Q000494:pharmacology*; D008297:Male; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar
chemical_list: D007529:Isoflavones; C018986:ipriflavone