title: Integrin β3 is not critical for neutrophil recruitment in a mouse model of pneumococcal pneumonia.
abstract: Streptococcus pneumoniae is one of the most common causes of bacterial pneumonias in humans. Neutrophil migration into lungs infected with S. pneumoniae is central to the host defense but the mechanisms of neutrophil recruitment, as mediated by S. pneumoniae, into lungs are incompletely understood. Therefore, we have assessed the role of integrin αvβ3 by evaluating its subunit β3 in a mouse model of lung inflammation induced by S. pneumonia. Integrin subunit β3 knockout (β3(-/-)) and wild-type (WT) mice were intratracheally instilled with either S. pneumoniae or saline. Other groups of WT mice were treated intraperitoneally with 25 μg or 50 μg of antibody against integrin β3 or with isotype-matched antibody at 1 h before instillation of S. pneumoniae. Mice were killed 24 h after infection. Flow cytometry confirmed the absence or presence of integrin subunit β3 on peripheral blood neutrophils in β3(-/-) or WT mice, respectively. Neutrophil numbers in bronchoalveolar lavage (BAL) from infected β3(-/-) and WT mice showed no differences. Neutrophil numbers in BAL of infected WT mice treated with β3 antibody were lower compared with those without antibody but similar to those of mice administered isotype-matched antibody. Many neutrophils were present in the perivascular spaces of the lungs in β3(-/-) mice. Lungs from infected β3(-/-) mice had negligible mitogen-activated protein kinase expression compared with those of infected WT mice. Thus, integrin β3 or its heterodimer αvβ3 is not critical for neutrophil migration into lungs infected with S. pneumoniae.
authors.affiliation: Department of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK, Canada.
authors.forename: Kyathanahalli S
authors.initials: KS
authors.lastname: Janardhan
authors.forename: Chandrashekhar
authors.initials: C
authors.lastname: Charavaryamath
authors.forename: Gurpreet K
authors.initials: GK
authors.lastname: Aulakh
authors.forename: Baljit
authors.initials: B
authors.lastname: Singh
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005434:Flow Cytometry; D007150:Immunohistochemistry; D039661:Integrin beta3 / Q000378:metabolism*; D007958:Leukocyte Count; D008168:Lung / Q000201:enzymology / Q000382:microbiology / Q000473:pathology; D051379:Mice; D020556:Neutrophil Infiltration / Q000276:immunology*; D009504:Neutrophils / Q000378:metabolism; D010766:Phosphorylation; D011014:Pneumonia / Q000097:blood / Q000150:complications / Q000382:microbiology / Q000473:pathology; D011018:Pneumonia, Pneumococcal / Q000150:complications / Q000276:immunology* / Q000382:microbiology* / Q000473:pathology; D021122:Protein Subunits / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D039661:Integrin beta3; D021122:Protein Subunits; D048051:p38 Mitogen-Activated Protein Kinases