title: The diastereomers of mannosylerythritol lipids have different interfacial properties and aqueous phase behavior, reflecting the erythritol configuration.
abstract: Mannosylerythritol lipids (MELs) produced by yeasts are one of the most promising glycolipid biosurfactants. There are two MEL diastereomers, in which the configurations of the erythritol moieties are opposite. The 4-O-β-D-mannopyranosyl-(2S,3R)-erythritol (S-form) or 4-O-β-D-mannopyranosyl-(2R,3S)-erythritol (R-form) is the hydrophilic domain. In this study, we prepared S- and R-form MEL homologs with similar fatty acyl groups, and compared their interfacial properties. Among the four diastereomers (S-MEL-B and -D/R-MEL-B and -D), R-form MELs showed a higher critical aggregation concentration and hydrophilicity compared to the corresponding S-form. R-form MELs also efficiently formed relatively large vesicles compared to S-form. Moreover, we estimated the binary phase diagram of the MEL-water system and compared the aqueous phase behavior among the four diastereomers. The present MELs self-assembled into a lamellar (L(α)) structure at all concentration ranges. Meanwhile, the one-phase L(α) region of R-form MELs was wider than those of S-form MELs. R-form MELs may maintain more water between the polar layers in accordance with an extension of the interlayer spacing. These results suggest that the differences in MEL carbohydrate configurations significantly affect interfacial properties, self-assembly, and hydrate ability.
authors.affiliation: Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5-2, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
authors.forename: Tokuma
authors.initials: T
authors.lastname: Fukuoka
authors.forename: Takashi
authors.initials: T
authors.lastname: Yanagihara
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Imura
authors.forename: Tomotake
authors.initials: T
authors.lastname: Morita
authors.forename: Hideki
authors.initials: H
authors.lastname: Sakai
authors.forename: Masahiko
authors.initials: M
authors.lastname: Abe
authors.forename: Dai
authors.initials: D
authors.lastname: Kitamoto
mesh_terms: D002236:Carbohydrate Conformation; D004896:Erythritol / Q000737:chemistry*; D006017:Glycolipids / Q000737:chemistry*; D013237:Stereoisomerism; D013329:Structure-Activity Relationship; D013499:Surface Properties; D013501:Surface-Active Agents / Q000737:chemistry; D014867:Water / Q000737:chemistry*
chemical_list: D006017:Glycolipids; D013501:Surface-Active Agents; C439671:mannosylerythritol lipid; D014867:Water; D004896:Erythritol