title: Down-regulation of CRMP-1 in patients with epilepsy and a rat model.
abstract: The Collapsin Response Mediator Protein-1 (CRMP-1) is a brain specific protein identified as a signaling molecule of Semaphorin-3A and act as axon repellent guidance factor in nervous system. Recent studies indicated that axon guidance molecules may play a role in synaptic reorganization in the adult brain and thereby promote epileptogenesis. This study aimed to investigate expression pattern of CRMP-1 in epileptogenesis. Using double immunofluorescence labeling, immunohistochemistry and western blot analysis, we looked into the CRMP-1 expression in temporal neocortex from patients with temporal lobe epilepsy (TLE) and histological normal temporal neocortex from the controls. We also studied the expression pattern of CRMP-1 in hippocampus and adjacent cortex of a TLE rat model on 6, 24, 72 h, 1, 2 weeks, 1 month, and 2 months post-seizure, and from control rats. CRMP-1 was mainly expressed in the neuronal cytoplasm in the temporal lobe of intractable TLE patients, which was co-expressed with -2. CRMP-1 expression was downregulated in temporal neocortical of TLE patients. In addition, in pilocarpine-induced animal model of epilepsy, CRMP-1 dynamically decreased in a range of 2 months. Thus, our results indicate that CRMP-1 may be involved in the development of TLE.
authors.affiliation: Department of Neurology, The First Affiliated Hospital, Chongqing Medical University, 1 You Yi Road, Chongqing, 400016, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Luo
authors.forename: Kebin
authors.initials: K
authors.lastname: Zeng
authors.forename: Chengqu
authors.initials: C
authors.lastname: Zhang
authors.forename: Min
authors.initials: M
authors.lastname: Fang
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Zhang
authors.forename: Qiong
authors.initials: Q
authors.lastname: Zhu
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Wang
authors.forename: Guojun
authors.initials: G
authors.lastname: Chen
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D004195:Disease Models, Animal; D015536:Down-Regulation; D004827:Epilepsy / Q000503:physiopathology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D055815:Young Adult; D000098855:Collapsin Response Mediator Protein 1
chemical_list: D009419:Nerve Tissue Proteins; C102924:CRMP1 protein, human; D000098855:Collapsin Response Mediator Protein 1