title: Naturally co-infected boars with both porcine reproductive and respiratory syndrome virus and porcine circovirus type 2.
abstract: In this study, the humoral response against porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2), the presence of the virus in semen and serum and the genetic characteristics of the virus detected in 15 boars from a commercial farm were analysed. The results showed that 53% of the boars presented anti-PRRSV antibodies and 100% presented anti-PCV2 antibodies. Porcine reproductive and respiratory syndrome virus was detected in 43% of the boars and 73% were positive to PCV2. The complete ORF5 gene of PRRSV of 14 samples and a fragment of the ORF2 gene of PCV2 of 22 samples were sequenced. Porcine reproductive and respiratory syndrome virus analysis revealed <92% identity in viruses from semen and serum of two boars, whereas in the rest of the boars the identity was >97.5%. As for PCV2, two boars presented an identity <95% in serum and semen and the rest had an identity >96%. The results showed that PRRSV- and PCV2-naturally infected boars can be found, and at least two different strains of viruses from semen and serum can be detected.
authors.affiliation: Laboratorio de Inmunología, Centro de Investigación en Alimentación y Desarrollo, A.C., Hermosillo, Sonora, México.
authors.forename: A
authors.initials: A
authors.lastname: Burgara-Estrella
authors.forename: M
authors.initials: M
authors.lastname: Montalvo-Corral
authors.forename: A
authors.initials: A
authors.lastname: Bolaños
authors.forename: H
authors.initials: H
authors.lastname: Ramírez-Mendoza
authors.forename: O
authors.initials: O
authors.lastname: Valenzuela
authors.forename: J
authors.initials: J
authors.lastname: Hernández
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D018173:Circoviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D060085:Coinfection / Q000453:epidemiology / Q000662:veterinary; D004279:DNA, Viral / Q000032:analysis; D008297:Male; D019318:Porcine Reproductive and Respiratory Syndrome / Q000453:epidemiology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D015995:Prevalence; D012367:RNA, Viral / Q000032:analysis; D012661:Semen / Q000821:virology; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology*
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D012367:RNA, Viral