title: Staphylococcus aureus proteins differentially recognized by the ovine immune response in mastitis or nasal carriage.
abstract: Staphylococcus aureus is an opportunistic pathogen in dairy ruminants where it is found in healthy carriage and can be a major cause of mastitis. A better knowledge of the host-pathogen interactions is needed to tackle this serious animal health problem. This study aimed at identifying S. aureus proteins differentially expressed by S. aureus in nasal colonization versus mastitis. Serological proteome analysis (SERPA) was used to examine protein samples prepared from culture supernatants of S. aureus strains originally isolated from gangrenous mastitis and nasal carriage (O11) or subclinical mastitis (O46) and to compare patterns of immune-reactive proteins. These staphylococcal proteins were revealed by sera obtained from ewes suffering from S. aureus mastitis and by sera obtained from healthy nulliparous ewes (i.e. no lactation and no mastitis or other symptoms) that were nasally colonized by S. aureus. Altogether 49 staphylococcal immune-reactive proteins were identified in this study. Patterns of proteins revealed by sera from infected- or healthy carrier- animals were comparable and analysis singled out one immune-reactive protein, N-acetylmuramyl-L-alanine amidase, which was recognized by each of the 6 sera from infected animals, when tested individually, and not by the sera of healthy carriers. This is the first study that compares the S. aureus seroproteome in colonization versus mastitis context in ruminants. These results open avenues for studies aiming at a better understanding of the balance between infection and commensal lifestyle in this opportunistic pathogen and at new prevention strategies.
authors.affiliation: INRA, UMR1253, Science et Technologie du Lait et de l'Œuf, F-35042 Rennes, France.
authors.forename: Nubia
authors.initials: N
authors.lastname: Seyffert
authors.forename: Caroline
authors.initials: C
authors.lastname: Le Maréchal
authors.forename: Julien
authors.initials: J
authors.lastname: Jardin
authors.forename: John A
authors.initials: JA
authors.lastname: McCulloch
authors.forename: Fabio R
authors.initials: FR
authors.lastname: Rosado
authors.forename: Anderson
authors.initials: A
authors.lastname: Miyoshi
authors.forename: Sergine
authors.initials: S
authors.lastname: Even
authors.forename: Gwenaël
authors.initials: G
authors.lastname: Jan
authors.forename: Nadia
authors.initials: N
authors.lastname: Berkova
authors.forename: Eric
authors.initials: E
authors.lastname: Vautor
authors.forename: Richard
authors.initials: R
authors.lastname: Thiéry
authors.forename: Vasco
authors.initials: V
authors.lastname: Azevedo
authors.forename: Yves
authors.initials: Y
authors.lastname: Le Loir
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000276:immunology* / Q000378:metabolism; D002353:Carrier State; D005260:Female; D008413:Mastitis / Q000276:immunology*; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000276:immunology / Q000378:metabolism; D009666:Nose / Q000382:microbiology; D020543:Proteome; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology* / Q000382:microbiology; D034561:Sheep, Domestic / Q000276:immunology* / Q000382:microbiology; D013203:Staphylococcal Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000276:immunology / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D020543:Proteome; D009238:N-Acetylmuramoyl-L-alanine Amidase