title: Transmission, diagnosis, and recommendations for control of Pseudoloma neurophilia infections in laboratory zebrafish (Danio rerio) facilities.
abstract: The microsporidium Pseudoloma neurophilia represents a considerable challenge for laboratory zebrafish (Danio rerio) facilities. In 2010, P. neurophilia infections were diagnosed in zebrafish from 74% of the facilities that submitted fish to the Zebrafish International  Resource Center (ZIRC) pathology service, and this organism remains the most commonly diagnosed pathogen in submitted fish. Accordingly, many of the ZIRC pathology service consultations deal with control and prevention of microsporidiosis. Here we describe observations and experiments performed  at the ZIRC elucidating aspects of P. neurophilia transmission in zebrafish colonies. We then review current knowledge about P. neurophilia transmission and diagnosis. Considering this information, we present recommendations for control of P. neurophilia in zebrafish facilities.
authors.affiliation: Zebrafish International Resource Center, University of Oregon, Eugene, Oregon, USA. katy@zebrafish.org
authors.forename: Katrina N
authors.initials: KN
authors.lastname: Murray
authors.forename: Mathew
authors.initials: M
authors.lastname: Dreska
authors.forename: Andrzej
authors.initials: A
authors.lastname: Nasiadka
authors.forename: Miranda
authors.initials: M
authors.lastname: Rinne
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Matthews
authors.forename: Carrie
authors.initials: C
authors.lastname: Carmichael
authors.forename: Justin
authors.initials: J
authors.lastname: Bauer
authors.forename: Zoltan M
authors.initials: ZM
authors.lastname: Varga
authors.forename: Monte
authors.initials: M
authors.lastname: Westerfield
mesh_terms: D000822:Animal Husbandry / Q000379:methods*; D000818:Animals; D000830:Animals, Laboratory; D017931:DNA Primers / Q000235:genetics; D005393:Fish Diseases / Q000175:diagnosis* / Q000382:microbiology* / Q000517:prevention & control / Q000635:transmission; D006652:Histological Techniques / Q000662:veterinary; D016814:Microsporidia / Q000235:genetics*; D016881:Microsporidiosis / Q000175:diagnosis / Q000517:prevention & control / Q000635:transmission / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D015027:Zebrafish
chemical_list: D017931:DNA Primers