title: Acaricidal activity of petroleum ether extract of neem (Azadirachta indica) oil and its four fractions separated by column chromatography against Sarcoptes scabiei var. cuniculi larvae in vitro.
abstract: The petroleum ether extract of neem oil and its four fractions separated by column chromatography was diluted at different concentrations with liquid paraffin. The acaricidal bioassay was conducted using a dipping method. The results indicated that the median lethal concentration (LC50) of the petroleum ether extract (at the concentration of 500.0ml/l) was 70.9ml/l, 24h after treatment. At concentrations of 500.0, 250.0, 125.0, 62.5 and 31.2ml/l, the median lethal times (LT50) of the petroleum ether extract were 8.7, 8.8, 10.8, 11.5 and 13.1h, respectively. Thin-layer chromatography (TLC) showed that the petroleum ether extract of neem oil separated into four fractions (F1-F4). Acaricidal activity of 68.3% and 100.0% in the F2 and F4 was confirmed. These results suggest that petroleum ether extracts of neem oil and its four fractions possess useful acaricidal activity in vitro.
authors.affiliation: College of Animal Medicine, Sichuan Agricultural University, Ya'an 625014, PR China.
authors.forename: Yunxia
authors.initials: Y
authors.lastname: Deng
authors.forename: Dongxia
authors.initials: D
authors.lastname: Shi
authors.forename: Zhongqiong
authors.initials: Z
authors.lastname: Yin
authors.forename: Jianhong
authors.initials: J
authors.lastname: Guo
authors.forename: Renyong
authors.initials: R
authors.lastname: Jia
authors.forename: Jiao
authors.initials: J
authors.lastname: Xu
authors.forename: Xu
authors.initials: X
authors.lastname: Song
authors.forename: Cheng
authors.initials: C
authors.lastname: Lv
authors.forename: Qiaojia
authors.initials: Q
authors.lastname: Fan
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Liang
authors.forename: Fei
authors.initials: F
authors.lastname: Shi
authors.forename: Gang
authors.initials: G
authors.lastname: Ye
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
mesh_terms: D056810:Acaricides; D000473:Alkanes; D000818:Animals; D031601:Azadirachta / Q000737:chemistry*; D002850:Chromatography, Gel / Q000379:methods; D005989:Glycerides / Q000737:chemistry*; D007814:Larva; D011336:Probability; D011817:Rabbits; D012044:Regression Analysis; D012520:Sarcoptes scabiei; D012532:Scabies / Q000469:parasitology / Q000662:veterinary; D013729:Terpenes / Q000737:chemistry*
chemical_list: D056810:Acaricides; D000473:Alkanes; D005989:Glycerides; D013729:Terpenes; C002443:neem oil; C004544:naphtha