title: Comparative impact on islet isolation and transplant outcome of the preservation solutions Institut Georges Lopez-1, University of Wisconsin, and Celsior.
abstract: BACKGROUND
  Institut Georges Lopez-1 (IGL-1) is a preservation solution similar to University of Wisconsin (UW) with reversed Na/K contents. In this study, we assessed the impact of IGL-1, UW, and Celsior (CS) solutions on islet isolation and transplant outcome.
  
  
  METHODS
  We retrospectively analyzed 376 islet isolations from pancreases flushed and transported with IGL-1 (n=95), UW (n=204), or CS (n=77). We determined isolation outcome and β-cell function in vitro. Transplanted patients were divided into three groups depending on preservation solution of pancreas, and islet graft function was assessed by decrease in daily insulin needs, C-peptide/glucose ratios, β-scores, and transplant estimated function at 1- and 6-month follow-up.
  
  
  RESULTS
  IGL-1, UW, and CS groups were similar according to donor age, body mass index, and pancreas weight. There was no difference in islet yields between the three groups. Success rates, transplant rates, β-cell secretory function, and viability were similar for all three groups. We observed no difference in decreased insulin needs, C-peptide glucose ratios, β-scores, and transplant estimated function at 1- and 6-month follow-up between IGL-1, UW, and CS groups.
  
  
  CONCLUSIONS
  Our study shows that IGL-1 is equivalent to UW or CS solutions for pancreas perfusion and cold storage before islet isolation and transplantation.
authors.affiliation: Cell Isolation and Transplantation Center, Department of Surgery, Geneva University Hospitals and University of Geneva, Geneva, Switzerland. nadja.niclauss@hcuge.ch
authors.forename: Nadja
authors.initials: N
authors.lastname: Niclauss
authors.forename: Anne
authors.initials: A
authors.lastname: Wojtusciszyn
authors.forename: Philippe
authors.initials: P
authors.lastname: Morel
authors.forename: Sandrine
authors.initials: S
authors.lastname: Demuylder-Mischler
authors.forename: Coralie
authors.initials: C
authors.lastname: Brault
authors.forename: Geraldine
authors.initials: G
authors.lastname: Parnaud
authors.forename: Frederic
authors.initials: F
authors.lastname: Ris
authors.forename: Domenico
authors.initials: D
authors.lastname: Bosco
authors.forename: Lionel
authors.initials: L
authors.lastname: Badet
authors.forename: Pierre-Yves
authors.initials: PY
authors.lastname: Benhamou
authors.forename: Thierry
authors.initials: T
authors.lastname: Berney
mesh_terms: D000241:Adenosine / Q000627:therapeutic use; D000328:Adult; D000493:Allopurinol / Q000627:therapeutic use; D000704:Analysis of Variance; D015415:Biomarkers / Q000097:blood; D001786:Blood Glucose / Q000378:metabolism; D002096:C-Peptide / Q000097:blood; D016009:Chi-Square Distribution; D003920:Diabetes Mellitus / Q000097:blood / Q000188:drug therapy / Q000601:surgery*; D004187:Disaccharides / Q000627:therapeutic use; D004573:Electrolytes / Q000627:therapeutic use; D005260:Female; D005971:Glutamates / Q000627:therapeutic use; D005978:Glutathione / Q000627:therapeutic use; D006442:Glycated Hemoglobin / Q000378:metabolism; D006639:Histidine / Q000627:therapeutic use; D006801:Humans; D007004:Hypoglycemic Agents / Q000627:therapeutic use; D007328:Insulin / Q000627:therapeutic use; D007515:Islets of Langerhans / Q000187:drug effects* / Q000378:metabolism; D016381:Islets of Langerhans Transplantation; D008297:Male; D008353:Mannitol / Q000627:therapeutic use; D008875:Middle Aged; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions / Q000627:therapeutic use*; D011887:Raffinose / Q000627:therapeutic use; D012189:Retrospective Studies; D013997:Time Factors; D046509:Tissue Culture Techniques; D020858:Tissue and Organ Harvesting / Q000379:methods*; D016896:Treatment Outcome
chemical_list: D000241:Adenosine; D000493:Allopurinol; D015415:Biomarkers; D001786:Blood Glucose; D002096:C-Peptide; D004187:Disaccharides; D004573:Electrolytes; D005971:Glutamates; D005978:Glutathione; D006442:Glycated Hemoglobin; D006639:Histidine; D007004:Hypoglycemic Agents; D007328:Insulin; D008353:Mannitol; D019188:Organ Preservation Solutions; D011887:Raffinose; C088453:Celsior; C504757:IGL-1 solution; C055206:University of Wisconsin-lactobionate solution; C517652:hemoglobin A1c protein, human