title: Bi-layered collagen nano-structured membrane prototype (collagen matrix 10826(®)) for oral soft tissue regeneration: an "in vitro" study.
abstract: OBJECTIVE
  To evaluate fundamental cell functions, such as adhesion, IL-6 production and proliferation of human gingival keratinocytes cultured on a newly engineered collagen matrix (CM-10826) and to assess the degree of specific biocompatibility of this new device.
  
  
  METHODS
  Primary cultures of human keratinocytes were derived "in vitro" from biopsies of independent donors. Their true epithelial origin was ensured by the expression of cytokeratin 14. Adhesion, proliferation and production of IL-6 cytokine was then measured in the presence or absence of CM-10826 activity or of its relevant components.
  
  
  RESULTS
  Functional tests revealed that keratinocytes adhered to CM-10826 and up-regulated their basal IL-6 production. The type of keratinocytes used expressed cytokeratin 14. Proliferation experiments demonstrated that the best cellular response was observed in the presence of Collagen I, the main component of CM-10826. No undesired effects were observed as for keratinocyte viability, morphology or differentiation.
  
  
  CONCLUSIONS
  Our results demonstrate that CM-10826 has a favourable biological effect on the "in vitro" response of gingival keratinocytes in terms of IL-6 production, cell growth and adhesion. These findings may encourage a possible use of this collagen membrane as a tissue which, alone, may substitute for autologous gingival grafts thereby overcoming the limitations of autologous tissue.
authors.affiliation: Department of Surgical Sciences, University of Verona, Verona, Italy.
authors.forename: Pier Francesco
authors.initials: PF
authors.lastname: Nocini
authors.forename: Guglielmo
authors.initials: G
authors.lastname: Zanotti
authors.forename: Roberto
authors.initials: R
authors.lastname: Castellani
authors.forename: Silvia
authors.initials: S
authors.lastname: Grasso
authors.forename: Maria Giulia
authors.initials: MG
authors.lastname: Cristofaro
authors.forename: Daniele
authors.initials: D
authors.lastname: De Santis
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D005881:Gingiva / Q000166:cytology; D016557:Guided Tissue Regeneration, Periodontal / Q000379:methods*; D006801:Humans; D066298:In Vitro Techniques; D015850:Interleukin-6 / Q000378:metabolism; D015603:Keratinocytes / Q000378:metabolism / Q000502:physiology*; D008854:Microscopy, Electron; D049329:Nanostructures; D010641:Phenotype; D013552:Swine
chemical_list: D001672:Biocompatible Materials; D015850:Interleukin-6; C586523:collagen matrix 10826; D003094:Collagen