title: High dimensional and high resolution pulse sequences for backbone resonance assignment of intrinsically disordered proteins.
abstract: Four novel 5D (HACA(N)CONH, HNCOCACB, (HACA)CON(CA)CONH, (H)NCO(NCA)CONH), and one 6D ((H)NCO(N)CACONH) NMR pulse sequences are proposed. The new experiments employ non-uniform sampling that enables achieving high resolution in indirectly detected dimensions. The experiments facilitate resonance assignment of intrinsically disordered proteins. The novel pulse sequences were successfully tested using δ subunit (20 kDa) of Bacillus subtilis RNA polymerase that has an 81-amino acid disordered part containing various repetitive sequences.
authors.affiliation: Faculty of Chemistry, University of Warsaw, Pasteura 1, 02-093 Warsaw, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Zawadzka-Kazimierczuk
authors.forename: Wiktor
authors.initials: W
authors.lastname: Koźmiński
authors.forename: Hana
authors.initials: H
authors.lastname: Sanderová
authors.forename: Libor
authors.initials: L
authors.lastname: Krásný
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011506:Proteins / Q000737:chemistry*
chemical_list: D011506:Proteins; D012321:DNA-Directed RNA Polymerases