title: Epidemiological aspects of visceral leishmaniasis in baft district, kerman province, southeast of iran.
abstract: BACKGROUND
  Visceral leishmaniasis (kala-azar) is an endemic disease in some areas of Iran. A cross- sectional study was conducted for sero-epidemiological survey of visceral leishmaniasis (VL) in Baft district from Kerman Province, southeast of Iran.
  
  
  METHODS
  Blood samples were collected from children up to 12 years old and 10% of adult population from Baft villages with a multi-stage randomized cluster sampling. In addition, blood samples were collected from 30 domestic dogs from the same areas. All the collected blood samples were tested by direct agglutination test (DAT) for the detection of anti-Leishmania antibodies in both human and dog using the cut-off value of ≥1:3200 and ≥1:320, respectively. Parasitological, molecular, and pathological were performed on infected dogs. Chi-square and Fisher exact tests were used to compare sero-prevalence values.
  
  
  RESULTS
  From 1476 collected human serum samples, 23 (1.55%) showed anti-Leishmania antibodies at titers of 1:800 and 1:1600 whereas 14 (0.95%) showed anti-Leishmania infantum antibodies at titers of ≥1:3200. No statistically significant difference was found between male (1.18%) and female (0.69%) sero-prevalence (P=0.330). Children of 5-8 years showed the highest sero-prevalence rate (3.22%). Seven out of 30 domestic dogs (23%) showed anti-Leishmania antibodies at titers ≥1:320. Leishmania infantum was identified in five infected dogs by nested - PCR assay.
  
  
  CONCLUSIONS
  It seems that visceral leishmaniasis is being endemic in southern villages of Baft district, southeast of Iran.
authors.affiliation: Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: H
authors.initials: H
authors.lastname: Mahmoudvand
authors.forename: M
authors.initials: M
authors.lastname: Mohebali
authors.forename: I
authors.initials: I
authors.lastname: Sharifi
authors.forename: H
authors.initials: H
authors.lastname: Keshavarz
authors.forename: H
authors.initials: H
authors.lastname: Hajjaran
authors.forename: B
authors.initials: B
authors.lastname: Akhoundi
authors.forename: S
authors.initials: S
authors.lastname: Jahanbakhsh
authors.forename: M
authors.initials: M
authors.lastname: Zarean
authors.forename: A
authors.initials: A
authors.lastname: Javadi