title: [MYETS1 recombinant expression in prokaryotic cells and deletion analysis in multiple myeloma cell lines].
abstract: OBJECTIVE
  To explore the down-expression mechanism of MYETS1 gene in multiple myeloma cell lines ARH-77 or KM3, and express MYETS1 gene in prokaryotic express system.
  
  
  METHODS
  The region of chromosome 13q14.3 in ARH-77 and KM3 was detected by FISH. MYETS1 gene was amplified by RT-PCR and cloned into prokaryotic expression vector pGEX-4T.
  
  
  RESULTS
  Positive consequence was acquired in 13q14.3 where MYETS1 located by FISH in ARH- 77 and KM3 cell lines. Bioinformatics indicated highly sequence homology between MYETS1 and LECT1, but excluded the homology of open reading frame between MYETS1 and that of LECT1 by RT-PCR. Myets1 protein was expressed and harvested successfully.
  
  
  CONCLUSIONS
  The region of chromosome 13q14.3 ,where MYETS1 gene located, was not defected in ARH-77 and KM3 cell lines. Down-expression of MYETS1 might be regulated by other mechanisms in multiple myeloma cell lines.
authors.affiliation: Institute of Life Science and Technology, Central South University, Changsha, China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Wang
authors.forename: Liping
authors.initials: L
authors.lastname: Hong
authors.forename: Yi
authors.initials: Y
authors.lastname: Pan
authors.forename: Shuiping
authors.initials: S
authors.lastname: Liu
authors.forename: Kunlu
authors.initials: K
authors.lastname: Wu
authors.forename: Lijun
authors.initials: L
authors.lastname: Tang
mesh_terms: D045744:Cell Line, Tumor; D002882:Chromosomes, Human, Pair 13 / Q000235:genetics*; D017353:Gene Deletion; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis* / Q000235:genetics; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D009101:Multiple Myeloma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D011994:Recombinant Proteins; C068675:CNMD protein, human