title: Effects of dietary inulin and heat-inactivated Bacillus subtilis on gilthead seabream (Sparus aurata L.) innate immune parameters.
abstract: In the present study, a feeding trial was conducted to evaluate the effect of inulin and heat-inactivated Bacillus subtilis, single or combined, on several innate immune activities of gilthead seabream (Sparus aurata). Forty-eight specimens were randomly assigned to four dietary treatments: 0 (control), inulin (10 g/kg, prebiotic group), B. subtilis (10(7) cfu/g, probiotic group), or B. subtilis + inulin (10(7) cfu/g + 10 g/kg, synbiotic group). After two and four weeks, six fish of each group were sampled, with the main innate immune parameters (natural haemolytic complement activity, serum and leucocyte peroxidase, phagocytosis, respiratory burst, and cytotoxic activities) being determined. Inulin or heat-inactivated B. subtilis failed to significantly stimulate the innate immune parameters assayed, although some activities showed no significant increase through these treatments. A combination of inulin and B. subtilis resulted in an increase of such parameters, with the haemolytic complement activity being the only one significantly stimulated. To conclude, inulin and B. subtilis, when administered as a synbiotic, have a synergistic effect and enhance some innate immune parameters of gilthead seabream.
authors.affiliation: University of Murcia, Faculty of Biology, Department of Cell Biology and Histology, Fish Innate Immune System Group, Campus de Espinardo, Cartagena, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Cerezuela
authors.forename: A
authors.initials: A
authors.lastname: Cuesta
authors.forename: J
authors.initials: J
authors.lastname: Meseguer
authors.forename: M A
authors.initials: MA
authors.lastname: Esteban
mesh_terms: D000818:Animals; D001412:Bacillus subtilis / Q000276:immunology*; D003165:Complement System Proteins / Q000276:immunology; D019587:Dietary Supplements; D004341:Drug Evaluation; D006358:Hot Temperature; D007113:Immunity, Innate; D007444:Inulin / Q000008:administration & dosage / Q000494:pharmacology*; D007962:Leukocytes / Q000201:enzymology; D010587:Phagocytosis; D016897:Respiratory Burst; D021541:Sea Bream / Q000276:immunology*; D058616:Synbiotics
chemical_list: D007444:Inulin; D003165:Complement System Proteins