title: Enhanced tumor treatment using biofunctional indocyanine green-containing nanostructure by intratumoral or intravenous injection.
abstract: Indocyanine green (ICG) is a conventional dye that can be used in clinical near-infrared (NIR) imaging, and it is also an effective light absorber for laser-mediated photothermal therapy. However, applications of ICG were limited due to its fast degradation in aqueous media and quick clearance from the body. Herein, an ICG-containing nanostructure, ICG-PL-PEG, was developed for photothermal therapy, which was self-assembled by ICG and phospholipid-polyethylene glycol (PL-PEG). Our in vitro and in vivo experiments demonstrated that ICG-PL-PEG suspension was more efficient in producing a NIR-dependent temperature increase than ICG alone, due to the increase of ICG monomers from the addition of PL-PEG to match the central wavelength of the 808 nm laser. When conjugated with integrin α(v)β(3) monoclonal antibody (mAb), ICG-PL-PEG could be selectively internalized and retained in target tumor cells. Irradiation of an 808 nm laser after intravenous administration of ICG-PL-PEG-mAb resulted in tumor suppression in mice, while ICG alone had only limited effect. This is the first time an ICG-containing nanostructure has been used through systemic administration to achieve an efficient in vivo photothermal effect for cancer treatment. Therefore, ICG-PL-PEG could be used as a fluorescent marker as well as a light-absorber for imaging-guided photothermal therapy. All the components of ICG-PL-PEG have been approved for human use. Therefore, this unique ICG-containing nanostructure has great potential in clinical applications.
authors.affiliation: MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Zheng
authors.forename: Feifan
authors.initials: F
authors.lastname: Zhou
authors.forename: Baoyan
authors.initials: B
authors.lastname: Wu
authors.forename: Wei R
authors.initials: WR
authors.lastname: Chen
authors.forename: Da
authors.initials: D
authors.lastname: Xing
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000627:therapeutic use; D045744:Cell Line, Tumor; D006801:Humans; D007208:Indocyanine Green / Q000737:chemistry*; D007275:Injections, Intravenous; D039302:Integrin alphaVbeta3 / Q000276:immunology; D051379:Mice; D018613:Microscopy, Confocal; D049329:Nanostructures / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*
chemical_list: D000911:Antibodies, Monoclonal; D039302:Integrin alphaVbeta3; D010743:Phospholipids; D011092:Polyethylene Glycols; D007208:Indocyanine Green