title: SNAI1 overexpression induces stemness and promotes ovarian cancer cell             invasion and metastasis.
abstract: Ovarian cancer is the fifth most common cancer among women worldwide. Detection             of metastasis of ovarian cancer is crucial for diagnosis and prolongs the life             of patients. This study focused on whether SNAI1 overexpression relates to invasion             of ovarian cancer in vitro and in vivo. Invasion, colony formation and wound healing             assays and flow cytometric analysis were performed to test the invasion and proliferation             of SKOV3 ovarian cancer cells after transfection. The effect of SNAI1 on ovarian             cancer in vivo was validated using a murine xenograft model. In vitro, SNAI1 upregulation             led to an increased percent of CD133+ SKOV3 cells and promoted SKOV3 cell invasion             and proliferation. In vivo, the SNAI1 overexpression group showed the highest             rate of tumor growth compared with SNAI2 and the control group (60 and 50%, respectively).             Our results show that SNAI1 expression induces an increase in the number of CD133+             cells, a change important for the epithelial to mesenchymal transition and the             proliferation in ovarian cancer. It is suggested that SNAI1 may serve as a novel             target for ovarian cancer prediction and therapy.
authors.affiliation: Center for Reproductive Medicine, Tianjin Central Hospital of Obstetrics and Gynecology, Tianjin Medical University, Tianjin 300100, PR China.
authors.forename: Zhen-Yu
authors.initials: ZY
authors.lastname: Lu
authors.forename: Rong
authors.initials: R
authors.lastname: Dong
authors.forename: Dong
authors.initials: D
authors.lastname: Li
authors.forename: Wen-Bo
authors.initials: WB
authors.lastname: Li
authors.forename: Feng-Qin
authors.initials: FQ
authors.lastname: Xu
authors.forename: Ying
authors.initials: Y
authors.lastname: Geng
authors.forename: Yun-Shan
authors.initials: YS
authors.lastname: Zhang
mesh_terms: D000071916:AC133 Antigen; D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D005260:Female; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D010455:Peptides / Q000235:genetics / Q000378:metabolism; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014183:Transplantation, Heterologous
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; C000606024:Prom1 protein, mouse; C000604407:SNAI1 protein, human; C000604408:SNAI2 protein, human; C000604429:Snai1 protein, mouse; C000604449:Snai2 protein, mouse; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors