title: [The application of fluorescence in situ hybridization to the peripheral-blood preparations].
abstract: The hematologic malignancy with specific chromosomal/genetic abnormality is separately classified by the WHO classification of tumors hematopoietic and lymphoid tissues. The chromosomal abnormalities, t (9; 22), t (8; 21), t (15; 17) and inv (16) are especially important for the establishment of therapeutic strategy and prognostication. We examined in this study, five cases were analyzed, because abnormal cells were recognized by the differential white blood count of the peripheral-blood and specific chromosomal abnormalities were suspected. Whether peripheral-blood preparations after May-Grünwald Giemsa staining could be used for fluorescence in situ hybridization (FISH). The fusion signals were detected in the high rate by using a peripheral-blood specimen in four cases, except for one case that had no specific chromosomal abnormality.
authors.affiliation: Division of Clinical Laboratory, Department of Medicine, Fukuoka University Hospital, 7-45-1 Nanakuma, Jonan-ku, Fukuoka 814-0180 Japan. mikuta@fukuoka-u.ac.jp
authors.forename: Mikihiro
authors.initials: M
authors.lastname: Ikuta
authors.forename: Akiko
authors.initials: A
authors.lastname: Ogami
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Takamatsu
authors.forename: Kenichi
authors.initials: K
authors.lastname: Anami
authors.forename: Ikuo
authors.initials: I
authors.lastname: Miura
authors.forename: Junko
authors.initials: J
authors.lastname: Kato
authors.forename: Hiromi
authors.initials: H
authors.lastname: Ishida
authors.forename: Yuko
authors.initials: Y
authors.lastname: Futata
authors.forename: Hironobu
authors.initials: H
authors.lastname: Kawashima
authors.forename: Akira
authors.initials: A
authors.lastname: Matsunaga
mesh_terms: D000328:Adult; D002648:Child; D002869:Chromosome Aberrations; D004801:Eosine Yellowish-(YS); D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000473:pathology; D015473:Leukemia, Promyelocytic, Acute / Q000473:pathology; D008751:Methylene Blue; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000473:pathology*
chemical_list: C043260:May-Grunwald Giemsa; D008751:Methylene Blue; D004801:Eosine Yellowish-(YS)