title: Involvement of miRNA in erythroid differentiation.
abstract: miRNAs are a family of small ncRNAs that regulate gene expression by targeting mRNAs in a sequence-specific manner, inducing translational repression or mRNA degradation. In this review, we present and discuss the available literature on the expression of miRNAs in erythroid cells. There are several experimental systems that can be employed for studies focusing on the relationship between miRNAs and erythroid differentiation, including human embryonic stem cells forced to erythroid differentiation, K562 and UT-7 cells induced to hemoglobin production by chemical compounds, erythropoietin-treated erythroid precursor cells from normal subjects or patients affected by hematological disease and in vivo systems, such as zebrafish embryos. Several miRNAs were identified as deeply involved in the erythroid phenotype, including miR-15a, miR-16-1, miR-126, miR-144, miR-451 and miR-210. Several functions related with erythroid cells were demonstrated to be regulated by these miRNAs, including maturation and proliferation of early erythroid cells, expression of fetal γ-globin genes and enucleation. These identified erythroid specific miRNAs represent the starting point to develop new protocols for miRNA therapeutics, based on both anti-miR molecules or miRNA replacement.
authors.affiliation: BioPharmaNet, Department of Biochemistry & Molecular Biology, University of Ferrara, Ferrara, Italy.
authors.forename: Nicoletta
authors.initials: N
authors.lastname: Bianchi
authors.forename: Cristina
authors.initials: C
authors.lastname: Zuccato
authors.forename: Alessia
authors.initials: A
authors.lastname: Finotti
authors.forename: Ilaria
authors.initials: I
authors.lastname: Lampronti
authors.forename: Monica
authors.initials: M
authors.lastname: Borgatti
authors.forename: Roberto
authors.initials: R
authors.lastname: Gambari
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D019070:Cell Lineage; D041905:Erythroid Cells / Q000166:cytology* / Q000378:metabolism; D005786:Gene Expression Regulation; D005914:Globins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D023421:Models, Animal
chemical_list: D035683:MicroRNAs; D005914:Globins