title: Statistical mixture design selective extraction of compounds with antioxidant activity and total polyphenol content from Trichilia catigua.
abstract: Statistical design mixtures of water, methanol, acetone and ethanol were used to extract material from Trichilia catigua (Meliaceae) barks to study the effects of different solvents and their mixtures on its yield, total polyphenol content and antioxidant activity. The experimental results and their response surface models showed that quaternary mixtures with approximately equal proportions of all four solvents provided the highest yields, total polyphenol contents and antioxidant activities of the crude extracts followed by ternary design mixtures. Principal component and hierarchical clustering analysis of the HPLC-DAD spectra of the chromatographic peaks of 1:1:1:1 water-methanol-acetone-ethanol mixture extracts indicate the presence of cinchonains, gallic acid derivatives, natural polyphenols, flavanoids, catechins, and epicatechins.
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Estadual de Maringá, BR-87020-900, Maringá, PR, Brazil.
authors.forename: Audrey Alesandra Stinghen Garcia
authors.initials: AA
authors.lastname: Lonni
authors.forename: Renata
authors.initials: R
authors.lastname: Longhini
authors.forename: Gisely Cristiny
authors.initials: GC
authors.lastname: Lopes
authors.forename: João Carlos Palazzo
authors.initials: JC
authors.lastname: de Mello
authors.forename: Ieda Spacino
authors.initials: IS
authors.lastname: Scarminio
mesh_terms: D000096:Acetone / Q000737:chemistry; D000975:Antioxidants / Q000302:isolation & purification* / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol / Q000737:chemistry; D029582:Meliaceae / Q000737:chemistry*; D000432:Methanol / Q000737:chemistry; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000302:isolation & purification* / Q000494:pharmacology; D059808:Polyphenols / Q000302:isolation & purification* / Q000494:pharmacology; D012997:Solvents / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D059808:Polyphenols; D012997:Solvents; D014867:Water; D000096:Acetone; D000431:Ethanol; D000432:Methanol