title: Spacial characteristics of pyrene degradation and soil microbial activity with the distance from the ryegrass (Lolium perenne L.) root surface in a multi-interlayer rhizobox.
abstract: To investigate rhizosphere effects on the biodegradation of pyrene with the distance away from root surface in the rhizosphere of ryegrass (Lolium perenne L.), a glasshouse experiment was conducted using a multi-interlayer rhizobox where ryegrass were grown in a soil spiked with pyrene. The largest and most rapid dissipation of pyrene in planted soil appeared at 2 mm zone from the root zone. The pyrene degradation gradient followed the order: near-rhizosphere>root compartment>far-rhizosphere soil zones. In contrast, there was no difference in pyrene concentration with distance in the unplanted soil. Dynamic changes of soil microbial biomass carbon (C(mic)) and the activities of both soil polyphenol oxidase and dehydrogenase were to some extent coincident with the degradation of pyrene with distance away from the root compartment in planted soils, which indicated the changes of soil microorganisms in different soil zones of rhizosphere were mainly responsible for the observed pyrene degradation. The largest C(mic) and activities of both soil polyphenol oxidase and dehydrogenase also occurred in near-rhizosphere, especially in 2mm zone from the root surface. The above results suggest that the effect of root proximity is important in the degradation of pyrene in ryegrass growing soil.
authors.affiliation: Research Center for Eco-Environmental Sciences, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, Zhejiang 310029, China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Xie
authors.forename: Min
authors.initials: M
authors.lastname: Liao
authors.forename: Shu
authors.initials: S
authors.lastname: Fang
authors.forename: Ying
authors.initials: Y
authors.lastname: Peng
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Chai
mesh_terms: D001673:Biodegradation, Environmental; D018533:Biomass; D004156:Catechol Oxidase / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D016014:Linear Models; D008129:Lolium / Q000737:chemistry* / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry / Q000378:metabolism; D059808:Polyphenols / Q000737:chemistry; D011721:Pyrenes / Q000737:chemistry* / Q000378:metabolism; D058441:Rhizosphere; D012987:Soil / Q000032:analysis; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis* / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D013499:Surface Properties
chemical_list: D059808:Polyphenols; D011721:Pyrenes; D012987:Soil; D012989:Soil Pollutants; D004156:Catechol Oxidase