title: Effect of crush and axotomy of phrenic nerves on oxidative stress in diaphragm muscle of rats.
abstract: BACKGROUND
  In this study we investigated the effect of crush and axotomy of phrenic nerves on oxidative stress and antioxidant enzyme activities in rat diaphragm muscle.
  
  
  METHODS
  The animals in the first group were not crushed or axotomized and served as controls. Phrenic nerves of the rats in the second and third groups were crushed or axotomized in the diaphragm muscle.
  
  
  RESULTS
  The malondialdehyde level increased in diaphragm muscles after both crush and axotomy. The antioxidant enzymes, such as superoxide dismutase, glutathione peroxidase, carbonic anhydrase, and catalase, decreased in diaphragm muscles after both crush and axotomy.
  
  
  CONCLUSIONS
  Crushing or axotomizing the phrenic nerves may produce oxidative stress in the diaphragm muscle of the rat by increasing lipid peroxidation and decreasing antioxidant enzyme activities.
authors.affiliation: Department of Thoracic Surgery, Faculty of Medicine, Yuzuncu Yıl University, 65100 Van, Turkey. sayirfuat@yahoo.com
authors.forename: Fuat
authors.initials: F
authors.lastname: Sayir
authors.forename: Servet
authors.initials: S
authors.lastname: Kavak
authors.forename: Ismail
authors.initials: I
authors.lastname: Meral
authors.forename: Halit
authors.initials: H
authors.lastname: Demir
authors.forename: Nurettin
authors.initials: N
authors.lastname: Cengiz
authors.forename: Ufuk
authors.initials: U
authors.lastname: Çobanoğlu
mesh_terms: D000818:Animals; D019771:Axotomy; D002256:Carbonic Anhydrases / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D003964:Diaphragm / Q000473:pathology / Q000503:physiopathology*; D004195:Disease Models, Animal; D005979:Glutathione Peroxidase / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009409:Nerve Crush; D018384:Oxidative Stress / Q000502:physiology*; D010523:Peripheral Nervous System Diseases / Q000209:etiology / Q000473:pathology*; D010791:Phrenic Nerve / Q000503:physiopathology*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D002256:Carbonic Anhydrases