title: Phylogenetic and molecular characterization of coxsackievirus A24 variant isolates from a 2010 acute hemorrhagic conjunctivitis outbreak in Guangdong, China.
abstract: BACKGROUND
  Acute hemorrhagic conjunctivitis is a common disease in China. As a notifiable disease, cases are registered by ophthalmologists on the AHC surveillance system. An AHC outbreak caused by CA24v was observed in Guangdong Province in 2007 by the National Disease Supervision Information Management System. Three years later, a larger outbreak occurred in Guangdong during the August-October period (2010). To characterize the outbreak and compare the genetic diversity of CA24v, which was determined to be the cause of the outbreak, the epidemiology and the molecular characterization of CA24v were analyzed in this study.
  
  
  RESULTS
  A total of 69,635 cases were reported in the outbreak. 73.5% of index cases originated from students, children in kindergarten and factory workers, with the ≦ 9 age group at the highest risk. The male to female ratio was 1.84:1 among 0-19 years. 56 conjunctival swabs were collected to identify the causative agent from five cities with the AHC outbreak. 30 virus strains were isolated, and two of the genomes had the highest identity values (95.8%) with CA24v genomes. Four CA24v genotypes were identified by phylogenetic analysis for the VP1 and 3C regions. CA24v which caused the outbreak belonged to genotype IV. Furthermore, full nucleotide sequences for four representative isolates in 2010 and 2007 were determined and compared. 20 aa mutations, two nt insertions and one nt deletion were observed in the open reading frame, with 5'- and 3'- UTR respectively between them.
  
  
  CONCLUSIONS
  CA24v was determined to be the pathogen causing the outbreak and belongs to genotype IV. VP1 is more informative than 3C(Pro) for describing molecular epidemiology and we hypothesize that accumulative mutations may have promoted the outbreak.
authors.affiliation: Institute of Microbiology, Center for Disease Control and Prevention of Guangdong, Guangzhou, Guangdong, 510300, People's Republic of China.
authors.forename: Wu
authors.initials: W
authors.lastname: De
authors.forename: Zheng
authors.initials: Z
authors.lastname: Huanying
authors.forename: Li
authors.initials: L
authors.lastname: Hui
authors.forename: Monagin
authors.initials: M
authors.lastname: Corina
authors.forename: Guo
authors.initials: G
authors.lastname: Xue
authors.forename: Liu
authors.initials: L
authors.lastname: Leng
authors.forename: Zeng
authors.initials: Z
authors.lastname: Hanri
authors.forename: Fang
authors.initials: F
authors.lastname: Ling
authors.forename: Mo
authors.initials: M
authors.lastname: Yanling
authors.forename: Zhou
authors.initials: Z
authors.lastname: Huiqiong
authors.forename: Zhang
authors.initials: Z
authors.lastname: Huan
authors.forename: Kou
authors.initials: K
authors.lastname: Jing
authors.forename: Long
authors.initials: L
authors.lastname: Caiyun
authors.forename: Hiromu
authors.initials: H
authors.lastname: Yoshida
authors.forename: Ke
authors.initials: K
authors.lastname: Changwen
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D016000:Cluster Analysis; D003232:Conjunctivitis, Acute Hemorrhagic / Q000453:epidemiology* / Q000821:virology; D003384:Coxsackievirus Infections / Q000453:epidemiology* / Q000821:virology; D004196:Disease Outbreaks; D004770:Enterovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D055815:Young Adult
chemical_list: D012367:RNA, Viral