title: [Effects of ionic-dissolution of sol-gel bioactive glasses on human dental pulp cells].
abstract: OBJECTIVE
  To evaluate the effects of ionic-dissolution of sol-gel bioactive glasses (BG) on proliferation and alkaline phosphatase (ALP) activity of  human dental pulp cells (HDPCs) .
  
  
  METHODS
  The primary HDPCs were isolated from intact premolar and third molars and were cultured in DMEM. Then  the 4 th generation of HDPCs were cultured in DMEM, which contained 1 g/L of one of 58S, Nano-58S, and 45S5 ionic-dissolution products. Meanwhile  HDPCs were cultured in DMEM without BG as control group. Proliferation of the cells was evaluated by MTT assay on days 1,3,5 and 7. ALP activity was  measured on day 14 using ALP Assay Kit.
  
  
  RESULTS
  Compared with the control group, HDPCs' proliferation in the Nano-58S group increased  significantly. HDPCs cultured in Nano-58S and the 58S groups showed higher ALP activity (P<0.01); but in 45S5 group showed an inhibition on the HDPCs'  proliferation (P<0.01) and lower ALP activity (P<0.05).
  
  
  CONCLUSIONS
  Present work has shown that Nano-58S and 58S sol-gel BG can promote the  proliferation and ALP activity of HDPCs.
authors.affiliation: Department of Cariology and Endodontology, Peking University School and Hospital of Stomatology, Beijing 100081, China.
authors.forename: Shan-shan
authors.initials: SS
authors.lastname: Mi
authors.forename: Yan-mei
authors.initials: YM
authors.lastname: Dong
authors.forename: Xue-jun
authors.initials: XJ
authors.lastname: Gao
mesh_terms: D000293:Adolescent; D000328:Adult; D000469:Alkaline Phosphatase / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D002516:Ceramics / Q000494:pharmacology*; D003782:Dental Pulp / Q000166:cytology* / Q000187:drug effects; D005260:Female; D005782:Gels / Q000494:pharmacology; D005898:Glass / Q000737:chemistry*; D006801:Humans; D007477:Ions; D008297:Male; D055815:Young Adult
chemical_list: D005782:Gels; D007477:Ions; C425330:bioactive glass 45S5; C448994:bioactive glass 58S; D000469:Alkaline Phosphatase