title: Chicken egg yolk antibodies (IgY) for detecting circulating antigens of Schistosoma japonicum.
abstract: BACKGROUND
  IgY isolated from egg yolk has been widely used in immunodiagnostic tests, including tests to detect circulating antigen (soluble egg antigen or SEA) of Schistosoma japonicum.
  
  
  RESULTS
  A sandwich ELISA was established using a combination of anti-S. japonicum SEA-IgY polyclonal antibodies and IgM monoclonal antibodies. To explore sensitivity and specificity of the sandwich ELISA, serum samples from 43 patients infected with S. japonicum were tested. All acute cases and 91.3% of the chronic cases showed a positive reaction. Only 5% of the control sera from healthy persons gave a positive response. Cross-reactions with antibodies to nine other parasites were rare.
  
  
  CONCLUSIONS
  The developed immunoassay is reasonably sensitive and specific. It could be used for field research and treatment efficacy assessments.
authors.affiliation: National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025, PR China. cyuchun2006@yahoo.com.cn
authors.forename: Yu-Chun
authors.initials: YC
authors.lastname: Cai
authors.forename: Jian
authors.initials: J
authors.lastname: Guo
authors.forename: Shao-Hong
authors.initials: SH
authors.lastname: Chen
authors.forename: Li-Guang
authors.initials: LG
authors.lastname: Tian
authors.forename: Peter
authors.initials: P
authors.lastname: Steinmann
authors.forename: Mu-Xin
authors.initials: MX
authors.lastname: Chen
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.forename: Lin
authors.initials: L
authors.lastname: Ai
authors.forename: Jia-Xu
authors.initials: JX
authors.lastname: Chen
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood* / Q000276:immunology; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth / Q000097:blood* / Q000276:immunology; D002645:Chickens; D002648:Child; D004530:Egg Yolk / Q000737:chemistry / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D007075:Immunoglobulin M; D007136:Immunoglobulins; D007159:Immunologic Tests; D008297:Male; D008875:Middle Aged; D011817:Rabbits; D012549:Schistosoma japonicum / Q000276:immunology* / Q000302:isolation & purification; D012554:Schistosomiasis japonica / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; C016512:IgY; D007075:Immunoglobulin M; D007136:Immunoglobulins