title: [The invasion ability and expressions of matrix metalloproteinase-13 and calcium-binding protein S100A4 are inhibited by hyperthermia in human Tca8113 cells].
abstract: OBJECTIVE
  To study the effect of hyperthermia on anti-invasion of Tca8113 and the expression change of matrix metalloproteinase-13 (MMP-13) and calcium-binding protein S100A4 (S100A4).
  
  
  METHODS
  Tca8113 cell pools were incubated at 43 degrees C for 0, 40, 80, 120 min, respectively, and at 37, 41, 43, 45 degrees C respectively for 80 min. The effect of high temperatures on the invasion ability of Tca8113 was measured in vitro. The slides of cells were made and incubated at 43 degrees C for 0, 40, 80, 120 min, respectively. Immunocytochemical method was employed for detecting the expression change of MMP-13 and S100A4 protein. Tca8113 cells were incubated at 43 degrees C for 0, 40, 80, 120 min respectively and at 37, 41, 43, 45 degrees C respectively for 80 min. Western blot method was conducted for detecting the expressionchange of MMP-13 and S100A4 protein.
  
  
  RESULTS
  As incubating time at higher temperature lasted, the proportion of the cells with invasion ability decreased. Except groups of 40 min and 80 min at 43 degrees C and 41, 43 degrees C for 80 min, the rest groups show significant statistic differences (P < 0.05). The expression intensity of MMP-13 and S100A4 proteins in Tca8113 cells would decrease as incubating time at higher temperature lasted. The content of MMP-13 and S100A4 proteins would decrease as incubating time at higher temperature lasted or incubating temperature increased. Except the groups of 40, 80 min at 43 degrees C and 41, 43 degrees C for 80 min, statistic differences were identified (P < 0.05).
  
  
  CONCLUSIONS
  The invasion of Tca8113 could be inhibited by hyperthermia. The mechanism of this effect may be due to protein expression inhibition of MMP-13 and S100A4.
authors.affiliation: Dept. of Stomatology, The Affiliated Hospital of Dali College, Dali 671000, China.
authors.forename: Liming
authors.initials: L
authors.lastname: He
authors.forename: Li
authors.initials: L
authors.lastname: Bian
authors.forename: Ruizhu
authors.initials: R
authors.lastname: Tang
authors.forename: Yongwen
authors.initials: Y
authors.lastname: He
mesh_terms: D002135:Calcium-Binding Proteins; D045744:Cell Line, Tumor; D006801:Humans; D006979:Hyperthermia, Induced; D053509:Matrix Metalloproteinase 13; D009418:S100 Proteins
chemical_list: D002135:Calcium-Binding Proteins; D009418:S100 Proteins; D053509:Matrix Metalloproteinase 13