title: Characterization of rice genes using a heterologous full-length cDNA expression system.
abstract: As a result of the progress in sequencing technology, many plant genomes have now been determined. Functional genomics is required to clarify gene function in many of these species. To identify useful genes easily and quickly, we have developed a FOX (full-length cDNA overexpressor) hunting system in which full-length cDNAs are overexpressed in Arabidopsis plants. This system was applied to high-throughput analysis of rice genes through heterologous expression in Arabidopsis (rice FOX Arabidopsis lines). We demonstrated that it is possible to carry out high-throughput analysis of gene function by utilizing rice FOX Arabidopsis lines. In this protocol, we describe how to isolate candidate rice FOX Arabidopsis lines and to determine the rice fl-cDNA that is responsible for the observed phenotype.
authors.affiliation: RIKEN Plant Science Center, Tsurumi-ku, Yokohama, Kanagawa, Japan.
authors.forename: Mieko
authors.initials: M
authors.lastname: Higuchi
authors.forename: Youichi
authors.initials: Y
authors.lastname: Kondou
authors.forename: Masaki
authors.initials: M
authors.lastname: Mori
authors.forename: Takanari
authors.initials: T
authors.lastname: Ichikawa
authors.forename: Minami
authors.initials: M
authors.lastname: Matsui
mesh_terms: D060054:Agrobacterium / Q000235:genetics; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism*; D018076:DNA, Complementary; D004926:Escherichia coli / Q000235:genetics; D005287:Ferredoxin-NADP Reductase / Q000096:biosynthesis / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D018014:Gene Transfer Techniques; D017343:Genes, Plant; D018745:Genome, Plant; D057166:High-Throughput Screening Assays; D012275:Oryza / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D014170:Transformation, Genetic; D025801:Ubiquitin / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D025801:Ubiquitin; D005287:Ferredoxin-NADP Reductase