title: Novel neo glycolipid: formulation into pegylated cationic liposomes and targeting of DNA lipoplexes to the hepatocyte-derived cell line HepG2.
abstract: Liver parenchymal cells are an important target for the treatment of several metabolic and viral disorders. Corrective gene delivery for this purpose is an avenue that is receiving increasing attention. In the present study, we report a novel neo glycolipid that may be formulated into cationic liposomes with or without poly(ethylene glycol) decoration. Lipoplexes formed with plasmid DNA are nuclease resistant and are targeted to the human hepatoblastoma cell line HepG2 by selective asialoglycoprotein receptor mediation. Transfection levels achieved by lipoplexes containing the targeting ligand cholesteryl-3β-N-(4-aminophenyl-β-D-galactopyranosyl) carbamate were sixfold greater than those obtained with similar but untargeted lipoplexes.
authors.affiliation: Department of Biochemistry, Non-Viral Gene Delivery Laboratory , University of KwaZulu-Natal, Westville Campus, Durban, South Africa.
authors.forename: Nicolisha
authors.initials: N
authors.lastname: Narainpersad
authors.forename: Moganavelli
authors.initials: M
authors.lastname: Singh
authors.forename: Mario
authors.initials: M
authors.lastname: Ariatti
mesh_terms: D037263:Asialoglycoprotein Receptor / Q000378:metabolism*; D004247:DNA / Q000737:chemistry*; D018390:Gene Targeting / Q000379:methods*; D006017:Glycolipids / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D008081:Liposomes / Q000138:chemical synthesis* / Q000737:chemistry / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D015394:Molecular Structure; D011092:Polyethylene Glycols; D014162:Transfection / Q000379:methods
chemical_list: D037263:Asialoglycoprotein Receptor; D006017:Glycolipids; D008081:Liposomes; D011092:Polyethylene Glycols; D004247:DNA