title: Isolation, mouse pathogenicity, and genotyping of Trypanosoma cruzi from an English Cocker Spaniel from Virginia, USA.
abstract: Trypanosoma cruzi was demonstrated in blood smears and heart tissue from a 5-year old, female, English Cocker Spaniel that had never been outside of the state of Virginia, USA. Plasma from the dog was positive in a commercially available immunochromatographic dipstick assay for T. cruzi and negative in an immunochromatographic dipstick assay for visceral Leishmania spp. The plasma from the dog had an indirect immunofluorescent antibody titer of 1:800 against epimastigotes of T. cruzi while the titer was 1:50 against promastigotes of L. infantum. The parasite was isolated from the blood in vitro from the dog (TcVT-1 isolate) and used to experimentally infect female C3H and ICR mice. The parasite was nonpathogenic for experimentally inoculated mice. DNA was isolated from parasites grown in vitro and used to determine that the genotype of T. cruzi present in the dog was genotype TcIV. This genotype is common in raccoons, Procyon lotor, in North America and suggests that raccoons may serve as reservoirs for canine infection.
authors.affiliation: Department of Biomedical Science and Pathology, Virginia Tech, 1401 Prices Fork Road, Blacksburg, VA 24061, USA.
authors.forename: Jay M
authors.initials: JM
authors.lastname: Patel
authors.forename: Alexa C
authors.initials: AC
authors.lastname: Rosypal
authors.forename: Kurt L
authors.initials: KL
authors.lastname: Zimmerman
authors.forename: William E
authors.initials: WE
authors.lastname: Monroe
authors.forename: Nammalwar
authors.initials: N
authors.lastname: Sriranganathan
authors.forename: Anne M
authors.initials: AM
authors.lastname: Zajac
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Yabsley
authors.forename: David S
authors.initials: DS
authors.lastname: Lindsay
mesh_terms: D000818:Animals; D014355:Chagas Disease / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D016054:DNA, Protozoan / Q000235:genetics; D004283:Dog Diseases / Q000453:epidemiology / Q000469:parasitology*; D004285:Dogs; D005260:Female; D005838:Genotype; D051379:Mice; D008809:Mice, Inbred C3H; D008813:Mice, Inbred ICR; D014349:Trypanosoma cruzi / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D014768:Virginia / Q000453:epidemiology
chemical_list: D016054:DNA, Protozoan