title: Fixed bed porous glass sphere (porosphere) bioreactors for animal cells.
abstract: Process intensity of fixed bed glass sphere culture systems is increased considerably by replacing solid glass spheres with open pore glass spheres. This technique demonstrates the possibility of having a system capable of both volumetric and cell density scale up and being suitable for substrate attached and suspension cells. The yields achieved for a number of attached cell lines (approximately 10(7)/ml) demonstrate an increase approaching one order of magnitude over solid glass spheres (approximately 10(6)/ml). Also suspension cells were successfully entrapped in the open pore structure with similar yields.
authors.affiliation: Biologics Division, PHLS CAMR, Porton Down, SP4 0JG, Salisbury, Wiltshire, United Kingdom.
authors.forename: D
authors.initials: D
authors.lastname: Looby
authors.forename: J B
authors.initials: JB
authors.lastname: Griffiths