title: [The efficacy research of multi-layer flat-plate bioartificial liver on acute liver failure].
abstract: OBJECTIVE
  To evaluate the efficacy of newly developed multi-layer flat-plate bioartificial liver in treatment of canines with acute liver failure.
  
  
  METHODS
  Porcine hepatocytes and bone marrow mesenchymal stem cells were cocultured in newly developed multi-layer flat-plate bioreactor. Acute liver failure in canine models was induced by D-galactosamine administration.Sixteen canine models were divided into two groups: treatment group (n = 8) and control group (n = 8). Biochemical parameters were determined for 7 days after treatment and liver specimens were collected for histological analysis.
  
  
  RESULTS
  Hepatic encephalopathy and general conditions were significantly improved in the treatment group, but no changes in the control group. Alanine aminotransferase was significantly decreased from (1512 ± 183) U/L to (86 ± 25) U/L in the treatment group, aspartate aminotransferase was significantly decreased from (1472 ± 365) U/L to (46 ± 11) U/L, lactate dehydrogenase was significantly decreased from (463 ± 76) U/L to (312 ± 84) U/L, total bilirubin was significantly decreased from (28.8 ± 6.2) µmol/L to (12.5 ± 3.6) µmol/L, ammonia was significantly decreased from (56 ± 15) µmol/L to (34 ± 10) µmol/L, and prothrombin time were significantly decreased in the treatment group but increased in the control group, albumin was improved in the treatment group but decreased in the control group. There were 5 canines survived in the treatment group but only 3 in the control group. But there was no difference on survival rates between the two group (P = 0.294).
  
  
  CONCLUSIONS
  The application of newly developed multi-layer flat-plate bioartificial liver system was effective in the treatment of canines with acute liver failure.
authors.affiliation: Department of Hepatobiliary Surgery, the Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008, China.
authors.forename: Xiao-lei
authors.initials: XL
authors.lastname: Shi
authors.forename: Xue-hui
authors.initials: XH
authors.lastname: Chu
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.forename: Bing
authors.initials: B
authors.lastname: Han
authors.forename: Jin-yang
authors.initials: JY
authors.lastname: Gu
authors.forename: Jiang-qiang
authors.initials: JQ
authors.lastname: Xiao
authors.forename: Jia-jun
authors.initials: JJ
authors.lastname: Tan
authors.forename: Yi-tao
authors.initials: YT
authors.lastname: Ding
mesh_terms: D000818:Animals; D019149:Bioreactors; D001854:Bone Marrow Cells / Q000166:cytology; D018920:Coculture Techniques; D004195:Disease Models, Animal; D004285:Dogs; D022781:Hepatocytes / Q000166:cytology; D017114:Liver Failure, Acute / Q000628:therapy*; D019164:Liver, Artificial