title: Distribution change of mast cells in human nasal polyps.
abstract: Recent studies have shown that mast cells are involved in pathophysiologic processes of chronic inflammation. However, little is known about the distribution of mast cells in nasal polyps, which is a chronic inflammatory disease of the upper airways. Biopsy specimens from patients with nasal polyps (n = 20) and control patients without nasal polyps (n = 8) were included in this study. The distribution of mast cells in nasal polyps was determined by immunohistochemistry. Meanwhile, we detected the expression of chemokines (CCL5, CCL11, CX3CL1, IL-8, IL-6) in the epithelial cells of normal nasal mucosa and nasal polyps. In addition, the expression of these chemokines was investigated by western bolting in airway epithelial cells line (A549 cells) under inflammatory condition. Mast cells migrated toward intraepithelium in nasal polyps and the expression of chemokines (CCL5, CCL11, CX3CL1, IL-8) was up-regulated in the epithelial cells of nasal polyps compared with normal nasal mucosa. The expression of chemokines was also up-regulated in A549 cells after Lipopolysaccharide (LPS)-treatment for 3 hr and 6 hr. Our findings showed that mast cells migrate toward intraepithelium in nasal polyps and the overexpression of chemokines (CCL5, CCL11, CX3CL1, IL-8) suggested that they might be responsible for mast cells migration. It implies that mast cell play potential roles in the development of nasal polyps.
authors.affiliation: Department of Immunology, Department of Biochemistry, Basic Medical College, Tianjin Medical University, Tianjin, People's Republic of China.
authors.forename: Guimin
authors.initials: G
authors.lastname: Zhang
authors.forename: Jie
authors.initials: J
authors.lastname: Shao
authors.forename: Chao
authors.initials: C
authors.lastname: Su
authors.forename: Xiulan
authors.initials: X
authors.lastname: Zhao
authors.forename: Xinting
authors.initials: X
authors.lastname: Wang
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Sun
authors.forename: Wenjie
authors.initials: W
authors.lastname: Shi
authors.forename: Peiyong
authors.initials: P
authors.lastname: Sun
authors.forename: Zhi
authors.initials: Z
authors.lastname: Yao
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000378:metabolism; D001706:Biopsy; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D054413:Chemokine CCL11 / Q000378:metabolism; D018946:Chemokine CCL5 / Q000378:metabolism; D054428:Chemokine CX3CL1 / Q000378:metabolism; D002633:Chemotaxis; D002681:China; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D015850:Interleukin-6 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D008407:Mast Cells / Q000276:immunology / Q000473:pathology*; D008875:Middle Aged; D009297:Nasal Mucosa / Q000276:immunology / Q000473:pathology; D009298:Nasal Polyps / Q000276:immunology / Q000473:pathology*; D020545:Respiratory Mucosa / Q000187:drug effects / Q000276:immunology / Q000473:pathology*; D013997:Time Factors; D015854:Up-Regulation; D055815:Young Adult
chemical_list: D015415:Biomarkers; C515456:CCL11 protein, human; C483024:CCL5 protein, human; C515538:CX3CL1 protein, human; C508606:CXCL8 protein, human; D054413:Chemokine CCL11; D018946:Chemokine CCL5; D054428:Chemokine CX3CL1; C508600:IL6 protein, human; D015850:Interleukin-6; D016209:Interleukin-8; D008070:Lipopolysaccharides