title: Characterization of immune modulating functions of γδ T cell subsets in a gnotobiotic pig model of human rotavirus infection.
abstract: We characterized immune modulating functions of porcine γδ T cell subsets in rotavirus infection using a gnotobiotic pig model of human rotavirus infection and sort-purified lymphocyte autologous co-cultures. We demonstrated that CD2+CD8- and CD2-CD8- γδ T cells have mainly pro-inflammatory function as evident by directly secreting IFN-γ or promoting CD4+ αβ T cell proliferation and IFN-γ production, whereas CD2+CD8+ γδ T cells mainly exert regulatory T cell function by expressing FoxP3, secreting IL-10 and TGF-β or increasing IL-10 and TGF-β production by CD4+ αβ T cells. γδ T cells responded to rotavirus infection by increasing TLR2, TLR3, TLR9 expression and IFN-γ and/or TGF-β production. The CD8- subsets likely differentiate into CD8+ subset by acquiring CD8 expression, explaining in part the apparently dual functions of CD2+CD8+ and CD2+CD8- subsets. Thus, both CD8+ and CD8- γδ T cell subsets can contribute to anti-rotavirus immunity and to the maintenance and restoration of intestinal and systemic homeostasis.
authors.affiliation: Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Integrated Life Science Building (0913), 1981 Kraft Dr, Blacksburg, VA 24061, USA.
authors.forename: Ke
authors.initials: K
authors.lastname: Wen
authors.forename: Tammy
authors.initials: T
authors.lastname: Bui
authors.forename: Guohua
authors.initials: G
authors.lastname: Li
authors.forename: Fangning
authors.initials: F
authors.lastname: Liu
authors.forename: Yanru
authors.initials: Y
authors.lastname: Li
authors.forename: Jacob
authors.initials: J
authors.lastname: Kocher
authors.forename: Lijuan
authors.initials: L
authors.lastname: Yuan
mesh_terms: D000818:Animals; D018801:CD2 Antigens / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000473:pathology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000473:pathology; D018920:Coculture Techniques; D004195:Disease Models, Animal; D005434:Flow Cytometry; D015870:Gene Expression; D005856:Germ-Free Life / Q000276:immunology; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis / Q000276:immunology; D016753:Interleukin-10 / Q000096:biosynthesis / Q000276:immunology; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000378:metabolism*; D012400:Rotavirus Infections / Q000276:immunology* / Q000473:pathology / Q000821:virology; D013552:Swine; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000473:pathology; D051193:Toll-Like Receptors / Q000235:genetics / Q000276:immunology; D016212:Transforming Growth Factor beta / Q000096:biosynthesis / Q000276:immunology
chemical_list: D018801:CD2 Antigens; D016692:Receptors, Antigen, T-Cell, gamma-delta; D051193:Toll-Like Receptors; D016212:Transforming Growth Factor beta; D016753:Interleukin-10; D007371:Interferon-gamma