title: Knockdown of HMGA1 expression by short/small hairpin RNA inhibits growth of ovarian carcinoma cells.
abstract: The aim of the current study was to investigate the influence of downregulating high-mobility group protein A1 (HMGA1) on the tumor gene and the mechanisms underlying the antitumor of HMGA1. The efficient short/small hairpin RNAs (shRNAs) of HMGA1 were constructed and transfected into human ovarian carcinoma OVCAR cells. The changes were identified by reverse transcription PCR (RT-PCR), Western blotting, methyl thiazolyl tetrazolium, and invasion assay. The knockdown of HMGA1 expression in OVCAR cells could obviously change cell morphology, decrease cell proliferation, and reduce invasion in vitro. BALB/C nude mice injected with OVCAR cells transfected HMGA1 shRNA showed a significantly lower tumor weight and volume than those in the control group. Taken together, HMGA1 knockdown could reduce the growth and metastasis potentials of OVCAR cells.
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Harbin University, Harbin, People's Republic of China.
authors.forename: Yinglan
authors.initials: Y
authors.lastname: Liu
authors.forename: Yingwei
authors.initials: Y
authors.lastname: Wang
authors.forename: Yaling
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jianhua
authors.initials: J
authors.lastname: Fu
authors.forename: Guangmei
authors.initials: G
authors.lastname: Zhang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D049109:Cell Proliferation / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D055785:Gene Knockdown Techniques; D025741:HMGA1a Protein / Q000096:biosynthesis / Q000172:deficiency* / Q000235:genetics*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010051:Ovarian Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D034741:RNA, Small Interfering / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013329:Structure-Activity Relationship; D014407:Tumor Cells, Cultured
chemical_list: D012333:RNA, Messenger; D034741:RNA, Small Interfering; D025741:HMGA1a Protein