title: Diverse sequence types of Klebsiella pneumoniae contribute to the dissemination of blaNDM-1 in India, Sweden, and the United Kingdom.
abstract: Clinical isolates of Klebsiella pneumoniae producing NDM-1 carbapenemase from India (n = 22), the United Kingdom (n = 13), and Sweden (n = 4) were subjected to multilocus sequence typing (MLST), automated repetitive sequence-based PCR (rep-PCR), serotyping, virulence gene screening, and plasmid replicon typing. The most frequently detected MLST sequence types (STs) were ST14 (n = 13; all serotype K2), ST11, ST149, ST231, and ST147. The correlation between MLST and automated rep-PCR was excellent. IncA/C was the most frequently detected plasmid replicon type (n = 14). ST14, ST11, and other successful clones may be important for the dissemination of bla(NDM-1).
authors.affiliation: Clinical Microbiology L2:02, Karolinska Institutet—MTC, Karolinska University Hospital, Stockholm, Sweden. christian.giske@karolinska.se
authors.forename: Christian G
authors.initials: CG
authors.lastname: Giske
authors.forename: Inga
authors.initials: I
authors.lastname: Fröding
authors.forename: Chowdhury Mehedi
authors.initials: CM
authors.lastname: Hasan
authors.forename: Agata
authors.initials: A
authors.lastname: Turlej-Rogacka
authors.forename: Mark
authors.initials: M
authors.lastname: Toleman
authors.forename: David
authors.initials: D
authors.lastname: Livermore
authors.forename: Neil
authors.initials: N
authors.lastname: Woodford
authors.forename: Timothy R
authors.initials: TR
authors.lastname: Walsh
mesh_terms: D016521:Electrophoresis, Gel, Pulsed-Field; D022761:Gene Transfer, Horizontal; D006801:Humans; D007194:India; D007711:Klebsiella pneumoniae / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D058885:Multilocus Sequence Typing; D058974:Phylogeography; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D012093:Replicon / Q000235:genetics; D012703:Serotyping; D013548:Sweden; D006113:United Kingdom; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001618:beta-Lactamases; C552864:beta-lactamase NDM-1