title: Senescence of fetal endothelial progenitor cell in pregnancy with idiopathic fetal growth restriction.
abstract: OBJECTIVE
  The aim of our study was to investigate the change of count and the status of cellular senescence in fetal endothelial progenitor cells (EPCs) obtained from the umbilical cord blood of women with fetal growth restriction (FGR).
  
  
  METHODS
  Fetal EPCs were obtained from thirty five normal and thirty pregnant women with FGR. Each EPC was characterized and counted. EPC differentiation time and outgrowth endothelial cell (OEC) colony formation assay, senescence-associated β-galactosidase (SA-β-gal) activity assay, and telomerase activity assay were performed.
  
  
  RESULTS
  Fetal EPC counts were significantly decreased in the FGR group compared with normal controls. In the FGR group, the EPC differentiation time was prolonged, OEC colonies were much less formed, the staining intensity of SA-β-gal was relatively increased and the telomerase activity of EPCs was significantly decreased, compared with normal pregnancy (p < 0.001 for all).
  
  
  CONCLUSIONS
  The fetal EPCs in FGR pregnancies were decreased, functionally impaired and senescently altered.
authors.affiliation: Department of Obstetrics and Gynecology, Konkuk University School of Medicine, Seoul, Korea.
authors.forename: Han Sung
authors.initials: HS
authors.lastname: Hwang
authors.forename: Young Guen
authors.initials: YG
authors.lastname: Kwon
authors.forename: Ja Young
authors.initials: JY
authors.lastname: Kwon
authors.forename: Yong
authors.initials: Y
authors.lastname: Won Park
authors.forename: Yong Sun
authors.initials: YS
authors.lastname: Maeng
authors.forename: Young Han
authors.initials: YH
authors.lastname: Kim
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D002469:Cell Separation; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000502:physiology*; D042783:Endothelial Cells / Q000166:cytology / Q000473:pathology* / Q000502:physiology*; D005260:Female; D005317:Fetal Growth Retardation / Q000000981:diagnostic imaging / Q000473:pathology* / Q000503:physiopathology; D053686:Fetal Stem Cells / Q000166:cytology / Q000473:pathology / Q000502:physiology*; D006801:Humans; D007231:Infant, Newborn; D011247:Pregnancy; D014463:Ultrasonography