title: Changes in the charged metabolite and sugar profiles of pasteurized and unpasteurized Japanese sake with storage.
abstract: Japanese sake (rice wine) is commonly heat treated (pasteurized) to maintain its quality. In this study, temporal changes in the metabolite profiles of pasteurized and unpasteurized sake were investigated during storage. Metabolomic analyses were conducted for eight sets of pasteurized and unpasteurized sake obtained from single process batches stored at 8 or 20 °C for 0, 1, 2, or 4 months. Capillary electrophoresis time-of-flight mass spectrometry and liquid chromatography tandem mass spectrometry were used to obtain charged metabolite and sugar profiles, respectively. The total amino acid concentration decreased with storage, and the decrease was faster in pasteurized sake than in unpasteurized. The organic acid concentrations were relatively constant in both types of sake. Peptide and glucose concentrations increased and polysaccharide concentrations decreased in unpasteurized sake, while they were relatively constant in pasteurized sake. Rather than stabilizing the sake metabolite profile during storage, pasteurization results in characteristic changes compared to unpasteurized sake.
authors.affiliation: Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata, Japan. msugi@sfc.keio.ac.jp
authors.forename: Masahiro
authors.initials: M
authors.lastname: Sugimoto
authors.forename: Miku
authors.initials: M
authors.lastname: Kaneko
authors.forename: Hiromi
authors.initials: H
authors.lastname: Onuma
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Sakaguchi
authors.forename: Masayo
authors.initials: M
authors.lastname: Mori
authors.forename: Shinobu
authors.initials: S
authors.lastname: Abe
authors.forename: Tomoyoshi
authors.initials: T
authors.lastname: Soga
authors.forename: Masaru
authors.initials: M
authors.lastname: Tomita
mesh_terms: D000596:Amino Acids / Q000032:analysis; D002241:Carbohydrates / Q000737:chemistry*; D061353:Food Storage / Q000379:methods*; D061352:Pasteurization; D014920:Wine / Q000032:analysis*
chemical_list: D000596:Amino Acids; D002241:Carbohydrates