title: A reversible near-infrared fluorescence probe for reactive oxygen species based on Te-rhodamine.
abstract: We have designed and synthesized a reversible near-infrared (NIR) fluorescence probe, 2-Me TeR, for reactive oxygen species (ROS), utilizing the redox properties of the tellurium (Te) atom. 2-Me TeR is oxidized to fluorescent 2-Me TeOR by various ROS, while the generated 2-Me TeOR is quickly reduced in the presence of glutathione to regenerate 2-Me TeR. This redox-induced reversible NIR-fluorescence response of 2-Me TeR allowed us to detect the endogenous production of ROS and subsequent homeostatic recovery of the intracellular reductive environment in hydrogen peroxide-stimulated HL-60 cells. This probe is expected to be useful for monitoring the dynamics of ROS production continuously in vivo.
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Koide
authors.forename: Mitsuyasu
authors.initials: M
authors.lastname: Kawaguchi
authors.forename: Yasuteru
authors.initials: Y
authors.lastname: Urano
authors.forename: Kenjiro
authors.initials: K
authors.lastname: Hanaoka
authors.forename: Toru
authors.initials: T
authors.lastname: Komatsu
authors.forename: Masahiro
authors.initials: M
authors.lastname: Abo
authors.forename: Takuya
authors.initials: T
authors.lastname: Terai
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Nagano
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D007259:Infrared Rays; D015336:Molecular Probe Techniques; D017382:Reactive Oxygen Species / Q000032:analysis*; D012235:Rhodamines / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D017382:Reactive Oxygen Species; D012235:Rhodamines