title: Angiopoietin-1-expressing adipose stem cells genetically modified with baculovirus nanocomplex: investigation in rat heart with acute infarction.
abstract: The objective of this study was to develop angiopoietin-1 (Ang1)-expressing genetically modified human adipose tissue derived stem cells (hASCs) for myocardial therapy. For this, an efficient gene delivery system using recombinant baculovirus complexed with cell penetrating transactivating transcriptional activator TAT peptide/deoxyribonucleic acid nanoparticles (Bac-NP), through ionic interactions, was used. It was hypothesized that the hybrid Bac- NP(Ang1) system can efficiently transduce hASCs and induces favorable therapeutic effects when transplanted in vivo. To evaluate this hypothesis, a rat model with acute myocardial infarction and intramyocardially transplanted Ang1-expressing hASCs (hASC-Ang1), genetically modified by Bac-NP(Ang1), was used. Ang1 is a crucial pro-angiogenic factor for vascular maturation and neovasculogenesis. The released hAng1 from hASC-Ang1 demonstrated profound mitotic and anti-apoptotic activities on endothelial cells and cardiomyocytes. The transplanted hASC-Ang1 group showed higher cell retention compared to hASC and control groups. A significant increase in capillary density and reduction in infarct sizes were noted in the infarcted hearts with hASC-Ang1 treatment compared to infarcted hearts treated with hASC or the untreated group. Furthermore, the hASC-Ang1 group showed significantly higher cardiac performance in echocardiography (ejection fraction 46.28% ± 6.3%, P < 0.001 versus control, n = 8) than the hASC group (36.35% ± 5.7%, P < 0.01, n = 8), 28 days post-infarction. The study identified Bac-NP complex as an advanced gene delivery vehicle for stem cells and demonstrated its potential to treat ischemic heart disease with high therapeutic index for combined stem cell-gene therapy strategy.
authors.affiliation: Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering, Faculty of Medicine, McGill University, Montreal, QC, Canada.
authors.forename: Arghya
authors.initials: A
authors.lastname: Paul
authors.forename: Madhur
authors.initials: M
authors.lastname: Nayan
authors.forename: Afshan Afsar
authors.initials: AA
authors.lastname: Khan
authors.forename: Dominique
authors.initials: D
authors.lastname: Shum-Tim
authors.forename: Satya
authors.initials: S
authors.lastname: Prakash
mesh_terms: D017667:Adipocytes / Q000378:metabolism / Q000502:physiology* / Q000637:transplantation*; D042683:Angiopoietin-1 / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D048708:Cell Growth Processes / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D008297:Male; D009203:Myocardial Infarction / Q000378:metabolism / Q000473:pathology / Q000601:surgery / Q000628:therapy*; D053758:Nanoparticles / Q000737:chemistry / Q000627:therapeutic use*; D051381:Rats; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells; D013318:Stroke Volume / Q000187:drug effects; D023822:Tissue Engineering / Q000379:methods; D019076:Transgenes; D014945:Wound Healing / Q000502:physiology
chemical_list: D042683:Angiopoietin-1