title: [Preliminary exploration in proteomic-based mechanistic analysis of patients with acute renal allograft rejection].
abstract: OBJECTIVE
  To explore the application of proteomics in the mechanistic analysis of acute rejection (AR).
  
  
  METHODS
  Quantified proteomics with isobaric tags for relative and absolute quantitation (iTRAQ) labeling was utilized to identify the protein profiling between the transplantation patients with (n = 5) or without AR (n = 8) from 2008 to 2010.
  
  
  RESULTS
  Among the 179 identified proteins, 66 proteins in AR patients had at least a 2-fold change as compared with those without AR. The results demonstrated the dominant processes and responses associated with inflammation and complement activation. It was consistent with the underlying immune rejection associated with AR. Moreover, the results also indicated that high-coagulation state existed in AR patients. A number of transcription factors were identified in AR patients, including nuclear factor-κB, signal transducer and activator of transcription 1, signal transducer and activator of transcription 3. The analysis of transcription regulation networks suggested that the cross-talks among these key transcription factors might play an important role in the acute response and activation of coagulation system.
  
  
  CONCLUSIONS
  The application of proteomics provides a new strategy of mechanistic analysis in AR.
authors.affiliation: Shanghai Key Laboratory of Organ Transplantation, Biomedical Research Center, Shanghai, China.
authors.forename: Duo-jiao
authors.initials: DJ
authors.lastname: Wu
authors.forename: Ming
authors.initials: M
authors.lastname: Xu
authors.forename: Dong
authors.initials: D
authors.lastname: Zhu
authors.forename: Rui-ming
authors.initials: RM
authors.lastname: Rong
authors.forename: Qun-ye
authors.initials: QY
authors.lastname: Tang
authors.forename: Xiang-dong
authors.initials: XD
authors.lastname: Wang
authors.forename: Tong-yu
authors.initials: TY
authors.lastname: Zhu
mesh_terms: D000328:Adult; D001777:Blood Coagulation; D005260:Female; D006084:Graft Rejection / Q000276:immunology*; D006801:Humans; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D014157:Transcription Factors / Q000032:analysis
chemical_list: D020543:Proteome; D014157:Transcription Factors