title: [On the use of FTA technology for collection, archieving, and molecular analysis of microsporidia dna from clinical stool samples].
abstract: The FTA technology was applied for sampling, archiving, and molecular analysis of the DNA isolated from stool samples to diagnose and identify microsporidia, the intracellular opportunistic parasites which induce malabsortion syndrome in immunosuppressed humans, particularly in patients with AIDS. Microsporidia DNA was successfully amplified in 6 of 50 stool samples of HIV-positive patients of the S. P. Botkin Memorial Infectious Disease Hospital (St. Petersburg) applied to FTA cards (FTA-Cars, Whatman Inc. Florham Park, NJ, USA). Amplicons (the fragments of rDNA) were directly sequenced, and microsporidia species--Encephalitozoon intestinalis, E. cuniculi, E. hellem, and Enterocytozoon bieneusi--were identified in Genbank by NCBI BLAST program. The FTA method of DNA immobilization is especially promising for epidemiological and field population studies which involve genotyping of microsporidia species and isolates.
authors.forename: O I
authors.initials: OI
authors.lastname: Sokolova
authors.forename: A V
authors.initials: AV
authors.lastname: Dem'ianov
authors.forename: L S
authors.initials: LS
authors.lastname: Bovers
authors.forename: E S
authors.initials: ES
authors.lastname: Did'e
authors.forename: Iu Ia
authors.initials: IuIa
authors.lastname: Sokolova
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000150:complications / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D000163:Acquired Immunodeficiency Syndrome / Q000150:complications / Q000276:immunology; D000327:Adsorption; D016172:DNA Fingerprinting / Q000379:methods*; D004271:DNA, Fungal / Q000235:genetics* / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000235:genetics* / Q000302:isolation & purification; D030541:Databases, Genetic; D005243:Feces / Q000382:microbiology; D006678:HIV / Q000276:immunology; D006801:Humans; D016867:Immunocompromised Host; D016814:Microsporidia / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016881:Microsporidiosis / Q000150:complications / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012426:Russia
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal