title: The role of aristolochene synthase in diphosphate activation.
abstract: Analysis of the role of amino acids involved in diphosphate binding in the Michaelis complex of aristolochene synthase from P. roqueforti (PR-AS) reveals mechanistic details about leaving group (PPi) activation and the nature of the active site acid.
authors.affiliation: School of Chemistry, Cardiff University, Main Building, Park Place, Cardiff CF10 3AT, UK.
authors.forename: Juan A
authors.initials: JA
authors.lastname: Faraldos
authors.forename: Veronica
authors.initials: V
authors.lastname: Gonzalez
authors.forename: Rudolf K
authors.initials: RK
authors.lastname: Allemann
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000378:metabolism; D018360:Crystallography, X-Ray; D011756:Diphosphates / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D015394:Molecular Structure; D010407:Penicillium / Q000201:enzymology; D012717:Sesquiterpenes / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D011756:Diphosphates; D012717:Sesquiterpenes; C054277:aristolochene