title: Rapid and sensitive detection of Mycobacterium ulcerans by use of a loop-mediated isothermal amplification test.
abstract: This work reports the design and evaluation of a rapid loop-mediated isothermal amplification test for detecting Mycobacterium ulcerans DNA based on the multicopy insertion sequence IS2404. The test is robust and specific with a detection limit equivalent to 20 copies of the target sequence (0.01 to 0.1 genome). The test has potential for the diagnosis of Buruli ulcer under field conditions.
authors.affiliation: School of Veterinary Sciences, The University of Queensland, Gatton Campus, Gatton, Queensland, Australia. z.njiru@uq.edu.au
authors.forename: Zablon K
authors.initials: ZK
authors.lastname: Njiru
authors.forename: Dorothy
authors.initials: D
authors.lastname: Yeboah-Manu
authors.forename: Timothy P
authors.initials: TP
authors.lastname: Stinear
authors.forename: Janet A M
authors.initials: JA
authors.lastname: Fyfe
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D054312:Buruli Ulcer / Q000175:diagnosis* / Q000382:microbiology; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D019911:Mycobacterium ulcerans / Q000235:genetics / Q000302:isolation & purification*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial