title: Real sample temperature: a critical issue in the experiments of nuclear resonant vibrational spectroscopy on biological samples.
abstract: There are several practical and intertangled issues which make the experiments of nuclear resonant vibrational spectroscopy (NRVS) on biological samples difficult to perform. The sample temperature is one of the most important issues. In NRVS the real sample temperatures can be very different from the readings on the temperature sensors. In this study the following have been performed: (i) citing and analyzing various existing NRVS data to assess the real sample temperatures during the NRVS measurements and to understand their trends with the samples' loading conditions; (ii) designing several NRVS measurements with (Et(4)N)[FeCl(4)] to verify these trends; and (iii) proposing a new sample-loading procedure to achieve significantly lower real sample temperatures and to balance among the intertangled experimental issues in biological NRVS measurements.
authors.affiliation: Department of Chemistry, University of California, 1 Shields Avenue, Davis, CA 95616, USA. hxwang2@lbl.gov
authors.forename: Hongxin
authors.initials: H
authors.lastname: Wang
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Yoda
authors.forename: Saeed
authors.initials: S
authors.lastname: Kamali
authors.forename: Zhao Hui
authors.initials: ZH
authors.lastname: Zhou
authors.forename: Stephen P
authors.initials: SP
authors.lastname: Cramer
mesh_terms: D003080:Cold Temperature; D007503:Iron Isotopes; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D011506:Proteins / Q000737:chemistry; D012997:Solvents; D013057:Spectrum Analysis / Q000379:methods*; D013696:Temperature; D014732:Vibration
chemical_list: D007503:Iron Isotopes; D011506:Proteins; D012997:Solvents