title: Genetic structure of Varroa destructor populations infesting Apis mellifera colonies in Argentina.
abstract: Although mitochondrial DNA mapping of Varroa destructor revealed the presence of several haplotypes, only two of them (Korean and Japanese haplotypes) were capable to infest Apis mellifera populations. Even though the Korean haplotype is the only one that has been reported in Argentina, these conclusions were based on mites sampled in apiaries from a specific geographical place (Buenos Aires province). To study mites from several sites of Argentina could reveal the presence of the Japanese genotype, especially considering sites near to Brazil, where Japanese haplotype was already detected. The aim of this work was to study the genetic structure of V. destructor populations from apiaries located in various provinces of Argentina, in order to determine the presence of different haplotypes. The study was carried out between January 2006 and December 2009. Phoretic adult Varroa mites were collected from honey bee workers sampled from colonies of A. mellifera located in Entre Ríos, Buenos Aires, Corrientes, Río Negro, Santa Cruz and Neuquén provinces. Twenty female mites from each sampling site were used to carry out the genetic analysis. For DNA extraction a  nondestructive method was used. DNA sequences were compared to Korean haplotype (AF106899) and Japanese haplotype (AF106897). All DNA sequences obtained from mite populations sampled in Argentina, share 98% of similitude with Korean Haplotype (AF106899). Taking into account these results, we are able to conclude that Korean haplotype is cosmopolite in Argentina.
authors.affiliation: Laboratorio de Artrópodos, Universidad Nacional de Mar del Plata, Mar del Plata, Argentina. biomaggi@gmail.com
authors.forename: M
authors.initials: M
authors.lastname: Maggi
authors.forename: S
authors.initials: S
authors.lastname: Medici
authors.forename: S
authors.initials: S
authors.lastname: Quintana
authors.forename: S
authors.initials: S
authors.lastname: Ruffinengo
authors.forename: J
authors.initials: J
authors.lastname: Marcángeli
authors.forename: P
authors.initials: P
authors.lastname: Gimenez Martinez
authors.forename: S
authors.initials: S
authors.lastname: Fuselli
authors.forename: M
authors.initials: M
authors.lastname: Eguaras
mesh_terms: D000818:Animals; D001483:Base Sequence; D001516:Bees / Q000469:parasitology*; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics; D003576:Electron Transport Complex IV / Q000737:chemistry / Q000235:genetics; D005260:Female; D014644:Genetic Variation; D006239:Haplotypes; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D057077:Varroidae / Q000201:enzymology / Q000235:genetics*
chemical_list: D004272:DNA, Mitochondrial; C038862:cytochrome o oxidase; D003576:Electron Transport Complex IV