title: The effect of platelet lysate supplementation of a dextran-based hydrogel on cartilage formation.
abstract: In situ gelating dextran-tyramine (Dex-TA) injectable hydrogels have previously shown promising features for cartilage repair. Yet, despite suitable mechanical properties, this system lacks intrinsic biological signals. In contrast, platelet lysate-derived hydrogels are rich in growth factors and anti-inflammatory cytokines, but mechanically unstable. We hypothesized that the advantages of these systems may be combined in one hydrogel, which can be easily translated into clinical settings. Platelet lysate was successfully incorporated into Dex-TA polymer solution prior to gelation. After enzymatic crosslinking, rheological and morphological evaluations were performed. Subsequently, the effect of platelet lysate on cell migration, adhesion, proliferation and multi-lineage differentiation was determined. Finally, we evaluated the integration potential of this gel onto osteoarthritis-affected cartilage. The mechanical properties and covalent attachment of Dex-TA to cartilage tissue during in situ gel formation were successfully combined with the advantages of platelet lysate, revealing the potential of this enhanced hydrogel as a cell-free approach. The addition of platelet lysate did not affect the mechanical properties and porosity of Dex-TA hydrogels. Furthermore, platelet lysate derived anabolic growth factors promoted proliferation and triggered chondrogenic differentiation of mesenchymal stromal cells.
authors.affiliation: Department of Tissue Regeneration, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, The Netherlands.
authors.forename: Liliana S
authors.initials: LS
authors.lastname: Moreira Teixeira
authors.forename: Jeroen C H
authors.initials: JC
authors.lastname: Leijten
authors.forename: Jos W H
authors.initials: JW
authors.lastname: Wennink
authors.forename: Anindita G
authors.initials: AG
authors.lastname: Chatterjea
authors.forename: Jan
authors.initials: J
authors.lastname: Feijen
authors.forename: Clemens A
authors.initials: CA
authors.lastname: van Blitterswijk
authors.forename: Pieter J
authors.initials: PJ
authors.lastname: Dijkstra
authors.forename: Marcel
authors.initials: M
authors.lastname: Karperien
mesh_terms: D001672:Biocompatible Materials; D001696:Biomechanical Phenomena; D001792:Blood Platelets / Q000737:chemistry* / Q000502:physiology; D002356:Cartilage / Q000254:growth & development*; D002448:Cell Adhesion; D002454:Cell Differentiation; D002465:Cell Movement; D019902:Chondrocytes / Q000187:drug effects / Q000502:physiology; D020219:Chondrogenesis / Q000187:drug effects / Q000502:physiology*; D018920:Coculture Techniques; D003911:Dextrans; D006133:Growth Substances / Q000008:administration & dosage; D006801:Humans; D020100:Hydrogels; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000502:physiology; D008855:Microscopy, Electron, Scanning; D010003:Osteoarthritis / Q000473:pathology / Q000503:physiopathology / Q000628:therapy; D023822:Tissue Engineering; D014439:Tyramine
chemical_list: D001672:Biocompatible Materials; D003911:Dextrans; D006133:Growth Substances; D020100:Hydrogels; D014439:Tyramine