title: Osteoclast stimulatory transmembrane protein and dendritic cell–specific transmembrane protein cooperatively modulate cell–cell fusion to form osteoclasts and foreign body giant cells.
abstract: Cell–cell fusion is a dynamic phenomenon promoting cytoskeletal reorganization and phenotypic changes. To characterize factors essential for fusion of macrophage lineage cells, we identified the multitransmembrane protein, osteoclast stimulatory transmembrane protein (OC-STAMP), and analyzed its function. OC-STAMP–deficient mice exhibited a complete lack of cell–cell fusion of osteoclasts and foreign body giant cells (FBGCs), both of which are macrophage-lineage multinuclear cells, although expression of dendritic cell specific transmembrane protein (DC-STAMP), which is also essential for osteoclast/FBGC fusion, was normal. Crossing OC-STAMP–overexpressing transgenic mice with OC-STAMP–deficient mice restored inhibited osteoclast and FBGC cell–cell fusion seen in OC-STAMP–deficient mice. Thus, fusogenic mechanisms in macrophage-lineage cells are regulated via OC-STAMP and DC-STAMP.
authors.affiliation: Department of Orthopedic Surgery, Keio University School of Medicine, 35 Shinano-machi, Shinjuku-ku, Tokyo, Japan.
authors.forename: Hiroya
authors.initials: H
authors.lastname: Miyamoto
authors.forename: Takayuki
authors.initials: T
authors.lastname: Suzuki
authors.forename: Yoshiteru
authors.initials: Y
authors.lastname: Miyauchi
authors.forename: Ryotaro
authors.initials: R
authors.lastname: Iwasaki
authors.forename: Tami
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Yuiko
authors.initials: Y
authors.lastname: Sato
authors.forename: Kana
authors.initials: K
authors.lastname: Miyamoto
authors.forename: Hiroko
authors.initials: H
authors.lastname: Hoshi
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Hashimoto
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Yoshida
authors.forename: Wu
authors.initials: W
authors.lastname: Hao
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Mori
authors.forename: Hiroya
authors.initials: H
authors.lastname: Kanagawa
authors.forename: Eri
authors.initials: E
authors.lastname: Katsuyama
authors.forename: Atsuhiro
authors.initials: A
authors.lastname: Fujie
authors.forename: Hideo
authors.initials: H
authors.lastname: Morioka
authors.forename: Morio
authors.initials: M
authors.lastname: Matsumoto
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Chiba
authors.forename: Motohiro
authors.initials: M
authors.lastname: Takeya
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Toyama
authors.forename: Takeshi
authors.initials: T
authors.lastname: Miyamoto
mesh_terms: D000818:Animals; D002459:Cell Fusion; D003433:Crosses, Genetic; D005260:Female; D015743:Giant Cells, Foreign-Body / Q000166:cytology / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000172:deficiency / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D050778:NFATC Transcription Factors / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010010:Osteoclasts / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D011485:Protein Binding
chemical_list: C499636:DC-STAMP protein, mouse; D008565:Membrane Proteins; D050778:NFATC Transcription Factors; D009419:Nerve Tissue Proteins; C494005:Nfatc1 protein, mouse; C527519:OC-STAMP protein, mouse