title: New approach for amino acid profiling in human plasma by selective fluorescence derivatization.
abstract: A new approach for the separation of 6-aminoquinolyl-carbamyl (AQC)-derivatized amino acids has been proposed. The chromatography used ion-pairing mechanism to increase the method selectivity. Mobile phase was based on triethylamine buffer containing N,N-dimethyloctylamine as a modifier. A number of factors, buffer composition and pH, counterion concentration, temperature and acetonitrile gradient profile, were optimized to achieve final chromatographic conditions. With the presented analytical method, the separation and identification of 34 AQC-amino acids and amino compounds present in human plasma is possible. The results of validation proved the applicability of the method for quantification of 27 amino acids in biological samples. The ultrafiltration proposed as deproteinization procedure gave repeatable and reliable results for the amino acids under investigation. This method introduced in routine testing can be a suitable tool for amino acid profiling in plasma including all aspects of clinical application.
authors.affiliation: Department of Biochemistry and Biopharmaceuticals, National Medicines Institute, 30/34 Chełmska str, 00-725, Warsaw, Poland. m-jaworska@il.waw.pl
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Jaworska
authors.forename: Marta
authors.initials: M
authors.lastname: Stańczyk
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Wilk
authors.forename: Gabriela
authors.initials: G
authors.lastname: Kłaczkow
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Anuszewska
authors.forename: Justyna
authors.initials: J
authors.lastname: Barzał
authors.forename: Piotr
authors.initials: P
authors.lastname: Rzepecki
mesh_terms: D000097:Acetonitriles; D000596:Amino Acids / Q000097:blood* / Q000737:chemistry; D000634:Aminoquinolines / Q000737:chemistry*; D002021:Buffers; D002219:Carbamates / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005021:Ethylamines; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015203:Reproducibility of Results; D013696:Temperature; D014462:Ultrafiltration
chemical_list: C078178:6-aminoquinolyl-N-hydroxysuccinimidyl carbamate; D000097:Acetonitriles; D000596:Amino Acids; D000634:Aminoquinolines; D002021:Buffers; D002219:Carbamates; D005021:Ethylamines; C016162:triethylamine; C032159:acetonitrile