title: Efficient bioconversion of l-threonine to 2-oxobutyrate using whole cells of Pseudomonas stutzeri SDM.
abstract: 2-Oxobutyrate (2-OBA) is an important intermediate with many applications in the drug and chemical industries. l-Threonine, an industrial fermentation product, could be used as a suitable starting material for the 2-OBA production. In this study, whole cells of Pseudomonas stutzeri SDM were confirmed to possess a good ability to convert l-threonine into 2-OBA. The bioconversion conditions were optimized for the 2-OBA production from l-threonine. Using 9.2g dry cell weight l(-1) of whole cells of strain SDM as biocatalyst, the biocatalytic process produced 2-OBA at a high concentration (25.6gl(-1)) with a high molar conversion rate (99.6%) at 6h from 30g1(-1) of l-threonine.
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China.
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.forename: Chao
authors.initials: C
authors.lastname: Gao
authors.forename: Bin
authors.initials: B
authors.lastname: Che
authors.forename: Cuiqing
authors.initials: C
authors.lastname: Ma
authors.forename: Zhaojuan
authors.initials: Z
authors.lastname: Zheng
authors.forename: Tong
authors.initials: T
authors.lastname: Qin
authors.forename: Ping
authors.initials: P
authors.lastname: Xu
mesh_terms: D001711:Biotransformation; D002087:Butyrates / Q000378:metabolism*; D044223:Pseudomonas stutzeri / Q000378:metabolism*; D013912:Threonine / Q000378:metabolism*
chemical_list: D002087:Butyrates; D013912:Threonine; C005087:alpha-ketobutyric acid