title: Evidence of bluetongue virus serotype 21 (BTV-21) divergence.
abstract: Bluetongue virus serotype 21 (BTV-21) was originally isolated from Australia, but has now been reported from India, Indonesia, China and Japan. We report the isolation, and sequencing of BTV-21 from India. The complete ORF sequence of VP2 gene of this isolate showed that it is closely related to recent BTV-21 isolates from Japan (93-94% identity), and distantly related to BTV-21 reference strain (86% identity). Our results, along with the available sequences of Japanese isolates, suggest that the currently circulating BTV-21 strains from India and Japan are divergent from the original strain(s) from Australia and shed light on designing molecular diagnostics for the detection of BTV.
authors.affiliation: Department of Veterinary Microbiology, College of Veterinary Science, Sri Venkateswara Veterinary University, Rajendranagar, Hyderabad, 500030, India.
authors.forename: B
authors.initials: B
authors.lastname: Susmitha
authors.forename: D
authors.initials: D
authors.lastname: Sudheer
authors.forename: Pavuluri Panduranga
authors.initials: PP
authors.lastname: Rao
authors.forename: Madala
authors.initials: M
authors.lastname: Uma
authors.forename: Gaya
authors.initials: G
authors.lastname: Prasad
authors.forename: P
authors.initials: P
authors.lastname: Minakshi
authors.forename: Nagendra R
authors.initials: NR
authors.lastname: Hegde
authors.forename: Y Narasimha
authors.initials: YN
authors.lastname: Reddy
mesh_terms: D000818:Animals; D001819:Bluetongue / Q000821:virology*; D001820:Bluetongue virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D036022:Capsid Proteins / Q000235:genetics*; D016000:Cluster Analysis; D014644:Genetic Variation; D005838:Genotype; D007194:India; D008969:Molecular Sequence Data; D058974:Phylogeography; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012756:Sheep
chemical_list: D036022:Capsid Proteins; D012367:RNA, Viral; C442520:VP2 protein, Bluetongue virus