title: Loss of bone strength is dependent on skeletal site in disuse osteoporosis in rats.
abstract: Intramuscular injection with botulinum toxin A (BTX) leads to a transient paralysis of the muscles, resulting in a rapid loss of muscle mass and function as well as rapid bone loss (disuse osteoporosis). The purpose of this study was to investigate the temporal development and the site specificity of BTX-induced immobilization on bone strength at five skeletal sites. Three-month-old rats (n = 108) were randomized into nine groups: one served as baseline, while four were injected with BTX and four with saline in the right hind-limb musculature. Animals were killed after 1, 2, 3, or 4 weeks. BTX-induced a significant loss of rectus femoris muscle mass (-61%) and muscle cell cross-sectional area (-59%) as well as bone strength at the femoral neck (-31%), femoral diaphysis (-6%), distal femoral metaphysis (-17%), proximal tibial metaphysis (-31%), and tibial diaphysis (-13%) after 4 weeks. Muscle atrophy occurred in parallel with the bone loss at the femoral neck and proximal tibia, whereas it occurred earlier than the bone loss at the other skeletal sites. At the proximal tibial metaphysis BTX significantly decreased BV/TV (-10%), trabecular thickness (-13%), and bone formation (MS/BS -25%, BFR/BS -50%) and increased osteoclast covered surfaces (+97%) after 4 weeks. In conclusion, BTX-induced a time-dependent loss of bone strength. Moreover, the loss of bone strength differed significantly at the five tested skeletal sites.
authors.affiliation: Department of Biomedicine, Aarhus University, Aarhus, Denmark. jesper@jst.ana.au.dk
authors.forename: Jesper Skovhus
authors.initials: JS
authors.lastname: Thomsen
authors.forename: Lisbeth Lydiksen
authors.initials: LL
authors.lastname: Christensen
authors.forename: Jens Bay
authors.initials: JB
authors.lastname: Vegger
authors.forename: Jens Randel
authors.initials: JR
authors.lastname: Nyengaard
authors.forename: Annemarie
authors.initials: A
authors.lastname: Brüel
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000378:metabolism*; D001905:Botulinum Toxins; D018482:Muscle, Skeletal / Q000503:physiopathology*; D009133:Muscular Atrophy / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology; D010012:Osteogenesis; D010024:Osteoporosis / Q000209:etiology / Q000378:metabolism* / Q000503:physiopathology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013977:Tibia / Q000503:physiopathology
chemical_list: D001905:Botulinum Toxins