title: [The protein expression profiles induced by trimethyltin chloride in Vero cells].
abstract: OBJECTIVE
  To explore the biomarkers and mechanism of kidney toxicity induced by trimethyltin chloride (TMT-Cl) through analyzing the differences of protein expression profiles between vero cells and vero cells exposed to TMT-Cl.
  
  
  METHODS
  The differences of protein expression levels of three paired samples of vero cells and vero cells exposed to TMT-Cl were compared by two-dimensional gel electrophoresis (2-DE) and liquid chromatography-electrospray ionization-linear trap quadrupole (LC-ESI-LTQ). The differences of expression levels of Annexin A1 and α-Tubulin proteins were validated with western blot assay, and the differences of mRNA expression levels of Annexin A1 and α-Tubulin genes were detected with quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR).
  
  
  RESULTS
  Fifteen spots of differential expression in protein profiles between vero cells and vero cells exposed to TMT-Cl were found, and 9 of these spots were identified by LC-ESI-LTQ. The expression levels of 3 proteins (Annexin A1,similar to RAN protein and a hypothetical protein) increased and the expression levels of 6 proteins(growth factor receptor-bound protein 10, tubulin alpha 6, heterogeneous nuclear ribonucleoprotein, similar to elongation factor SIII p15 subunit, S-adenosylhomocysteine hydrolase and a hypothetical protein) reduced. The expression levels of α-Tubulin protein and mRNA significantly decreased in vero cells exposed to TMT-Cl, as compared with vero cells (P < 0.01). The expression of Annexin A1 protein in all exposure groups was significantly up-regulated, the expression of Annexin A1 mRNA in the groups exposed to 25 and 50 µmol/L TMT-Cl was significantly down-regulated, and The expression of Annexin A1 mRNA in the group exposed to 100 µmol/L TMT-Cl was significantly up-regulated (P < 0.01).
  
  
  CONCLUSIONS
  The results of present study suggest that 9 proteins with differential expression detected by LC-ESI-LTQ may be related to the kidney toxicity induced by TMT-Cl, which can serve as the biomarkers of early diagnosis and therapeutic effect for the kidney toxicity induced by TMT-Cl.
authors.affiliation: Institute of Hygiene, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Xiao
authors.forename: Li-jin
authors.initials: LJ
authors.lastname: Zhu
authors.forename: Li
authors.initials: L
authors.lastname: Jv
authors.forename: Ya-ling
authors.initials: YL
authors.lastname: Qian
authors.forename: Xing
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D012333:RNA, Messenger / Q000235:genetics; D059467:Transcriptome; D014298:Trimethyltin Compounds / Q000633:toxicity*; D014404:Tubulin / Q000235:genetics / Q000378:metabolism*; D014709:Vero Cells
chemical_list: D012333:RNA, Messenger; D014298:Trimethyltin Compounds; D014404:Tubulin; C040533:trimethyltin chloride