title: Unraveling the global microRNAome responses to ionizing radiation in human embryonic stem cells.
abstract: MicroRNAs (miRNA) comprise a group of short ribonucleic acid molecules implicated in regulation of key biological processes and functions at the post-transcriptional level. Ionizing radiation (IR) causes DNA damage and generally triggers cellular stress response. However, the role of miRNAs in IR-induced response in human embryonic stem cells (hESC) has not been defined yet. Here, by using system biology approaches, we show for the first time, that miRNAome undergoes global alterations in hESC (H1 and H9 lines) after IR. Interrogation of expression levels of 1,090 miRNA species in irradiated hESC showed statistically significant changes in 54 genes following 1 Gy of X-ray exposures; global miRNAome alterations were found to be highly temporally and cell line--dependent in hESC. Time-course studies showed that the 16 hr miRNAome radiation response of hESC is much more robust compared to 2 hr-response signature (only eight genes), and may be involved in regulating the cell cycle. Quantitative real-time PCR performed on some miRNA species confirms the robustness of our miRNA microarray platform. Positive regulation of differentiation-, cell cycle-, ion transport- and endomembrane system-related processes were predicted to be negatively affected by miRNAome changes in irradiated hESC. Our findings reveal a fundamental role of miRNAome in modulating the radiation response, and identify novel molecular targets of radiation in hESC.
authors.affiliation: Nuclear Medicine Division, Department of Radiology and Imaging Sciences, Clinical Center, National Institutes of Health, Bethesda, Maryland, United States of America. sokolovm@mail.nih.gov
authors.forename: Mykyta V
authors.initials: MV
authors.lastname: Sokolov
authors.forename: Irina V
authors.initials: IV
authors.lastname: Panyutin
authors.forename: Ronald D
authors.initials: RD
authors.lastname: Neumann
mesh_terms: D002453:Cell Cycle / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics; D004307:Dose-Response Relationship, Radiation; D053595:Embryonic Stem Cells / Q000378:metabolism / Q000528:radiation effects*; D020869:Gene Expression Profiling; D006801:Humans; D017136:Ion Transport / Q000235:genetics; D007700:Kinetics; D035683:MicroRNAs / Q000502:physiology*; D011839:Radiation, Ionizing
chemical_list: D035683:MicroRNAs