title: Functional analysis of the role of eyes absent and sine oculis in the developing eye of the cricket Gryllus bimaculatus.
abstract: In the cricket Gryllus bimaculatus, a hemimetabolous insect, the compound eyes begin to form in the embryo and increase 5-6 fold in size during the postembryonic development of the nymphal stage. Retinal stem cells in the anteroventral proliferation zone (AVPZ) of the nymphal eye proliferate to increase retinal progenitors, which then differentiate to form new ommatidia in the anterior region of the eye. However, mechanisms underlying this type of eye formation have not been well elucidated yet. Here, we found that the homologues of the retinal determination transcription factor genes of eyes absent (eya) and sine oculis (so) are expressed during the cricket embryonic eye formation. eya is also expressed intensely in the AVPZ of the nymphal eye. To explore their functions, we performed knockdown by RNA interference (RNAi). Knockdown of Gb'eya resulted in loss of the embryonic eye. In the nymphal eye, RNAi against Gb'eya or Gb'so impaired retinal morphology by apparently transforming cornea structures into head cuticle. These results imply that Gb'eya and Gb'so are essential for the differentiation of the retinal progenitor cells and maintaining retinal structures during eye development.
authors.affiliation: Department of Life Systems, Institute of Technology and Science, University of Tokushima, 2-1 Minami-Jyosanjima-cho, Tokushima City 770-8506, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Takagi
authors.forename: Kazuki
authors.initials: K
authors.lastname: Kurita
authors.forename: Taiki
authors.initials: T
authors.lastname: Terasawa
authors.forename: Taro
authors.initials: T
authors.lastname: Nakamura
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Bando
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Moriyama
authors.forename: Taro
authors.initials: T
authors.lastname: Mito
authors.forename: Sumihare
authors.initials: S
authors.lastname: Noji
authors.forename: Hideyo
authors.initials: H
authors.lastname: Ohuchi
mesh_terms: D000818:Animals; D005123:Eye / Q000196:embryology* / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics / Q000502:physiology; D006135:Gryllidae; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D019476:Insect Proteins; D014157:Transcription Factors