title: A selenium analogue of firefly D-luciferin with red-shifted bioluminescence emission.
abstract: A selenium analogue of amino-D-luciferin, aminoseleno-D-luciferin, is synthesized and shown to be a competent substrate for the firefly luciferase enzyme. It has a red-shifted bioluminescence emission maximum at 600 nm and is suitable for bioluminescence imaging studies in living subjects.
authors.affiliation: Department of Chemistry, Stanford University, CA 94305-5080, USA.
authors.forename: Nicholas R
authors.initials: NR
authors.lastname: Conley
authors.forename: Anca
authors.initials: A
authors.lastname: Dragulescu-Andrasi
authors.forename: Jianghong
authors.initials: J
authors.lastname: Rao
authors.forename: W E
authors.initials: WE
authors.lastname: Moerner
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D048888:Fireflies / Q000201:enzymology; D049454:Firefly Luciferin / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D049409:Luciferases, Firefly / Q000378:metabolism; D008163:Luminescent Measurements; D008279:Magnetic Resonance Imaging; D051379:Mice; D008819:Mice, Nude; D009369:Neoplasms / Q000000981:diagnostic imaging; D011877:Radionuclide Imaging; D012643:Selenium / Q000737:chemistry*; D013379:Substrate Specificity; D014183:Transplantation, Heterologous
chemical_list: D049454:Firefly Luciferin; D049409:Luciferases, Firefly; D012643:Selenium