title: Displacement immunoassay for the detection of ochratoxin A using ochratoxin B modified glass beads.
abstract: We report here the development of a new assay for the detection of ochratoxin A (OTA) based on the use of its dechlorinated analogue, ochratoxin B (OTB), in a displacement immunoassay. OTB was immobilised on controlled-pore glass beads followed by the binding of anti-OTA antibody, with anti-IgG antibody peroxidase conjugate used as a label. In this manner, an original bio-sensing material was obtained. Upon incubation of this material with OTA, the toxin competes with OTB for the binding sites of the anti-OTA antibodies and releases the antibody-tagged peroxidase complex into the solution. Compared to classic competitive immunoassays, this newly developed displacement immunoassay presents a similar detection limit and assay time. Moreover, the detection, based on the activity of the horseradish peroxidase, is performed for the first time in situ using wine samples.
authors.affiliation: IMAGES EA, Université de Perpignan Via Domitia, France.
authors.forename: Vasilica
authors.initials: V
authors.lastname: Lates
authors.forename: Cheng
authors.initials: C
authors.lastname: Yang
authors.forename: Ionel Catalin
authors.initials: IC
authors.lastname: Popescu
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Marty
mesh_terms: D005506:Food Contamination / Q000032:analysis; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D009793:Ochratoxins / Q000032:analysis*; D014920:Wine / Q000032:analysis
chemical_list: D009793:Ochratoxins; C025589:ochratoxin A; C045354:ochratoxin B