title: Transformation of barley (Hordeum vulgare L.) by Agrobacterium tumefaciens infection of in vitro cultured ovules.
abstract: Agrobacterium-mediated transformation of in vitro cultured barley ovules is an attractive alternative to well-established barley transformation methods of immature embryos. The ovule culture system can be used for transformation with and without selection and has successfully been used to transform cultivars other than Golden Promise indicating minor genotype dependency. The method allows for the rapid and direct generation of high-quality transgenic plants where the transgenes are stably expressed and show Mendelian inheritance in subsequent generations.
authors.affiliation: Department of Genetics and Biotechnology, Aarhus University, Research Centre Flakkebjerg, Slagelse, Denmark. Inger.Holme@agrsci.dk
authors.forename: Inger Bæksted
authors.initials: IB
authors.lastname: Holme
authors.forename: Henrik
authors.initials: H
authors.lastname: Brinch-Pedersen
authors.forename: Mette
authors.initials: M
authors.lastname: Lange
authors.forename: Preben Bach
authors.initials: PB
authors.lastname: Holm
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D005838:Genotype; D049452:Green Fluorescent Proteins / Q000235:genetics; D001467:Hordeum / Q000235:genetics* / Q000382:microbiology; D030821:Plants, Genetically Modified; D012639:Seeds / Q000235:genetics; D046509:Tissue Culture Techniques; D014170:Transformation, Genetic
chemical_list: D004269:DNA, Bacterial; C036483:T-DNA; D049452:Green Fluorescent Proteins