title: [The role of CYP2E1 in the protection of garlic oil's from n-hexane-induced neurotoxicity].
abstract: OBJECTIVE
  To study the role of CYP2E1 in the protective effects and mechanism of garlic oil (GO) on the peripheral nerve injuries induced by n-hexane.
  
  
  METHODS
  Fifty male Wistar rats were randomly divided into five groups (n = 10): the control, the GO (80 mg/kg) control, the n-hexane (2000 mg/kg) model, the low dose GO (40 mg/kg) plus n-hexane, and the high dose GO (80 mg/kg) plus n-hexane groups. All rats were treated by intragastric administration 6 times a week for 10 weeks. The gait scores were determined every two weeks for monitoring the peripheral neurotrosis. All rats were sacrificed in 10 weeks, the activities and expression levels of hepatic CYP2E1 and 2, 5-HD in serum were examined.
  
  
  RESULTS
  As compared with control group, the content and activity of hepatic CYP2E1 in GO control group reduced by 83.1% and 48.3% respectively (P < 0.01), the content and activity of hepatic CYP2E1 in model group increased by 112.5% and 72.2% respectively (P < 0.01). As compared with model group, the contents of hepatic CYP2E1 in low dose and high dose GO groups reduced by 32.9% and 39.1% respectively, the activities of hepatic CYP2E1 in low dose and high dose GO groups reduced by 27.4% and 44.5% respectively (P < 0.01); the contents of serum 2,5-HD in low dose and high dose GO groups reduced by 47.7% and 78.7% respectively (P < 0.01). The gait scores in model, low dose and high dose GO groups were significantly lower than that in control group, but the gait scores in low dose and high dose GO groups were significantly lower than that in model group (P < 0.05).
  
  
  CONCLUSIONS
  Garlic oil can effectively reduce the peripheral neurotrosis induced by n-hexane due to the decreased content and activity of hepatic CYP2E1, resulting in the reduced formation of 2, 5-HD from n-hexane.
authors.affiliation: Institute of Toxicology, School of Public Health, Shandong University, Jinan 250012, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Bi
authors.forename: Jing-jing
authors.initials: JJ
authors.lastname: Chen
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Qiang-qiang
authors.initials: QQ
authors.lastname: Fu
authors.forename: Tao
authors.initials: T
authors.lastname: Zeng
authors.forename: Ke-qin
authors.initials: KQ
authors.lastname: Xie
mesh_terms: D000818:Animals; D019392:Cytochrome P-450 CYP2E1 / Q000378:metabolism*; D005737:Garlic; D006586:Hexanes / Q000633:toxicity*; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D010525:Peripheral Nerves / Q000187:drug effects / Q000473:pathology*; D010938:Plant Oils / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D006586:Hexanes; D010938:Plant Oils; C026385:n-hexane; D019392:Cytochrome P-450 CYP2E1