title: Toxicity assessment and vitellogenin expression in zebrafish (Danio rerio) embryos and larvae acutely exposed to bisphenol A, endosulfan, heptachlor, methoxychlor and tetrabromobisphenol A.
abstract: Organochlorine pesticides and brominated flame retardants, such as tetrabromobisphenol A and polybrominated diphenyl ethers, pose an environmental hazard owing to their persistence, low solubility and estrogenic effects, and concerns have been raised regarding their effects on aquatic biota. In the present study, zebrafish embryos and larvae were used as a model to investigate the sublethal and lethal effects of three different organochlorine pesticides, namely methoxychlor, endosulfan and heptachlor, as well as the flame retardant tetrabromobisphenol A, and its precursor compound bisphenol A. Preliminary data for chemical exposure tests were obtained by determining the 96 h median effective concentration EC50 (hatching rate) and 96 h median lethal concentration LC50 . Quantitative polymerase chain reaction was used to investigate the gene expression levels of the biomarker vitellogenin (vtg1) after 96 h exposures to 10, 25, 50 and 75% of the 96 h EC50 value for embryos and 96 h LC50 value for larvae. The use of vtg1 mRNA induction in zebrafish embryos and larvae was found to be a sensitive biomarker of exposure to these organic compounds, and was helpful in elucidating their adverse effects and setting water quality guidelines.
authors.affiliation: Biochemistry Program, School of Life Sciences, Faculty of Science, The Chinese University of Hong Kong, Sha Tin, N.T., Hong Kong, SAR, China.
authors.forename: Wing Shan
authors.initials: WS
authors.lastname: Chow
authors.forename: Winson Ka-Lun
authors.initials: WK
authors.lastname: Chan
authors.forename: King Ming
authors.initials: KM
authors.lastname: Chan
mesh_terms: D000199:Actins / Q000096:biosynthesis; D000395:Air Pollutants, Occupational / Q000633:toxicity*; D000465:Algorithms; D000818:Animals; D001559:Benzhydryl Compounds / Q000633:toxicity*; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D004625:Embryo, Nonmammalian / Q000187:drug effects*; D005411:Flame Retardants / Q000633:toxicity*; D006533:Heptachlor / Q000633:toxicity*; D007306:Insecticides / Q000633:toxicity*; D007814:Larva / Q000187:drug effects*; D007928:Lethal Dose 50; D008731:Methoxychlor / Q000633:toxicity*; D019055:No-Observed-Adverse-Effect Level; D010636:Phenols / Q000633:toxicity*; D011075:Polybrominated Biphenyls / Q000633:toxicity*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012989:Soil Pollutants / Q000633:toxicity*; D014819:Vitellogenins / Q000096:biosynthesis*; D015027:Zebrafish / Q000502:physiology*; D000098826:Bisphenol A Compounds
chemical_list: D000199:Actins; D000395:Air Pollutants, Occupational; D001559:Benzhydryl Compounds; D018076:DNA, Complementary; D005411:Flame Retardants; D006533:Heptachlor; D007306:Insecticides; D008731:Methoxychlor; D010636:Phenols; D011075:Polybrominated Biphenyls; D012333:RNA, Messenger; D012989:Soil Pollutants; D014819:Vitellogenins; C020806:tetrabromobisphenol A; C006780:bisphenol A; D000098826:Bisphenol A Compounds