title: Amphiphilic self-assembled "polymeric drugs": morphology, properties, and biological behavior of nanoparticles.
abstract: This article reports the fabrication and characterization of NPs based on the self-assembling of polymeric drugs with amphiphilic character synthetized from oleyl 2-acetamido-2-deoxy-α-d-glucopyranoside methacrylate and vinyl pyrrolidone (OAGMA-VP). NPs were spherical, with an apparent hydrodynamic diameter between 91 and 226 nm and with zeta potential values that ensure stability. Atomic concentrations of C, O, and N, determined by X-ray photoelectron spectroscopy (XPS) of NPs, compared well with the corresponding theoretical values. High resolution XPS C1s spectra suggest that the carbons bound to heteroatoms or carbonyl groups are preferentially situated on the surface of the NP samples. ToF-SIMS spectra analyzed by principal component analysis (PCA) indicated that ions coming from acetyl and oleyl groups of OAGMA play important roles in the outer structure of NPs. Water contact angle and surface tension values of NPs were characteristic of hydrophilic surfaces, confirming the location of VP sequences on the surface. Cell culture assays showed that copolymeric NPs did not compromise biocompatibility of human fibroblasts according to ISO standard, but they were cytotoxic on a human glioblastoma cell line (A-172).
authors.affiliation: Institute of Polymer Science and Technology , CSIC, C/Juan de la Cierva 3, 28006 Madrid, Spain.
authors.forename: María Luisa
authors.initials: ML
authors.lastname: López-Donaire
authors.forename: Eric M
authors.initials: EM
authors.lastname: Sussman
authors.forename: Mar
authors.initials: M
authors.lastname: Fernández-Gutiérrez
authors.forename: Antonio
authors.initials: A
authors.lastname: Méndez-Vilas
authors.forename: Buddy D
authors.initials: BD
authors.lastname: Ratner
authors.forename: Blanca
authors.initials: B
authors.lastname: Vázquez-Lasa
authors.forename: Julio
authors.initials: J
authors.lastname: San Román
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D005909:Glioblastoma / Q000188:drug therapy* / Q000473:pathology; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D009682:Magnetic Resonance Spectroscopy; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry* / Q000627:therapeutic use*; D056951:Photoelectron Spectroscopy; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology*; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D011108:Polymers