title: [1,25(OH)(2)D(3) influences endothelial cell proliferation, apoptosis and endothelial nitric oxide synthase expression of aorta in apolipoprotein E-deficient mice].
abstract: OBJECTIVE
  To study possible influences of 1,25(OH)(2)D(3) on endothelial cell proliferation, apoptosis and endothelial nitric oxide synthase (eNOS) expression of aorta in apolipoprotein E-deficient (apoE(-/-)) mice and to explore the relationship between vitamin D and atherosclerosis.
  
  
  METHODS
  Endothelial cell of aorta in apoE(-/-) mice were isolated and cultured, and the influence of 1,25(OH)(2)D(3) on endothelial cell proliferation were observed by MTT, apoptosis of cells were quantitated by terminal deoxynucleotidyl transferase mediated dUTP nick end labelling, Bcl-2 mRNA, fas mRNA and eNOS mRNA was detected by reverse transcription-polymerase chain reaction.
  
  
  RESULTS
  Endothelial cell proliferation rate of aorta did not significantly change in the two control groups (0.162 ± 0.031 vs. 0.158 ± 0.006, P > 0.05). Compared with control groups, 1,25(OH)(2)D(3) stimulated endothelial cell proliferation of aorta (P < 0.05), but endothelial cell proliferation rate did not significantly change in different 1,25(OH)(2)D(3) concentration groups [1,25(OH)(2)D(3) concentration: 10(-4)mol/L, 10(-5) mol/L, 10(-6) mol/L, 10(-7) mol/L, 10(-8) mol/L, endothelial cell proliferation rate: 0.189 ± 0.013 vs. 0.285 ± 0.011 vs. 0.296 ± 0.026 vs. 0.284 ± 0.017 vs. 0.233 ± 0.010, P > 0.05]. 1,25(OH)(2)D(3) research concentration as chosen as 10(-6) mol/L. In 1,25(OH)(2)D(3) 10(-6) mol/L group, the expression of Bcl-2, eNOS mRNA was significantly increased (0.78 ± 0.16 vs. 0.46 ± 0.21 vs. 0.42 ± 0.17, 0.56 ± 0.16 vs. 0.39 ± 0.13 vs. 0.35 ± 0.11, 0.46 ± 0.2 vs. 10.42 ± 0.17 vs. 0.78 ± 0.16, 0.79 ± 0.21 vs. 0.81 ± 0.20 vs. 0.43 ± 0.12), apoptotic index, Fas mRNA was significantly decreased (15.14 ± 3.19 vs. 18.94 ± 4.22 vs. 19.27 ± 4.58, 0.43 ± 0.12 vs.0.79 ± 0.21 vs. 0.81 ± 0.20)(P < 0.05). The quantity of eNOS gene expression was inversely associated with apoptosis index and Fas mRNA, was positively associated with Bcl-2 mRNA (r = -0.676, -0.758, 0.762, P < 0.01).
  
  
  CONCLUSIONS
  1,25(OH)(2)D(3) stimulated endothelial cell proliferation, inhibited apoptosis and increased eNOS expression of aorta in apoE(-/-) mice. These results may deepen understanding of the pathogenesis of atherosclerosis.
authors.affiliation: Department of Pediatrics, Hainan Provincial People's Hospital, Haikou, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xiang
authors.forename: Xiao-jie
authors.initials: XJ
authors.lastname: He
authors.forename: Yan-lin
authors.initials: YL
authors.lastname: Ma
authors.forename: Zhu-wen
authors.initials: ZW
authors.lastname: Yi
authors.forename: Yan
authors.initials: Y
authors.lastname: Cao
authors.forename: Shui-ping
authors.initials: SP
authors.lastname: Zhao
authors.forename: Jin-fu
authors.initials: JF
authors.lastname: Yang
authors.forename: Zhi-chao
authors.initials: ZC
authors.lastname: Ma
authors.forename: Ming
authors.initials: M
authors.lastname: Wu
authors.forename: Sheng-miao
authors.initials: SM
authors.lastname: Fu
authors.forename: Jian-lin
authors.initials: JL
authors.lastname: Ma
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Zheng
authors.forename: Hong
authors.initials: H
authors.lastname: Kang
mesh_terms: D000818:Animals; D001011:Aorta / Q000378:metabolism*; D001057:Apolipoproteins E / Q000172:deficiency*; D017209:Apoptosis / Q000187:drug effects*; D002117:Calcitriol / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism; D005260:Female; D008297:Male; D051379:Mice; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D001057:Apolipoproteins E; D012333:RNA, Messenger; D052250:Nitric Oxide Synthase Type III; C496315:Nos3 protein, mouse; D002117:Calcitriol