title: Chemoenzymatic probes for detecting and imaging fucose-α(1-2)-galactose glycan biomarkers.
abstract: The disaccharide motif fucose-α(1-2)-galactose (Fucα(1-2)Gal) is involved in many important physiological processes, such as learning and memory, inflammation, asthma, and tumorigenesis. However, the size and structural complexity of Fucα(1-2)Gal-containing glycans have posed a significant challenge to their detection. We report a new chemoenzymatic strategy for the rapid, sensitive detection of Fucα(1-2)Gal glycans. We demonstrate that the approach is highly selective for the Fucα(1-2)Gal motif, detects a variety of complex glycans and glycoproteins, and can be used to profile the relative abundance of the motif on live cells, discriminating malignant from normal cells. This approach represents a new potential strategy for biomarker detection and expands the technologies available for understanding the roles of this important class of carbohydrates in physiology and disease.
authors.affiliation: Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 East California Boulevard, Pasadena, California 91125, United States.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Chaubard
authors.forename: Chithra
authors.initials: C
authors.lastname: Krishnamurthy
authors.forename: Wen
authors.initials: W
authors.lastname: Yi
authors.forename: David F
authors.initials: DF
authors.lastname: Smith
authors.forename: Linda C
authors.initials: LC
authors.lastname: Hsieh-Wilson
mesh_terms: D015415:Biomarkers / Q000032:analysis*; D004187:Disaccharides / Q000737:chemistry*; D004798:Enzymes / Q000737:chemistry*; D015335:Molecular Probes; D011134:Polysaccharides / Q000737:chemistry*
chemical_list: D015415:Biomarkers; D004187:Disaccharides; D004798:Enzymes; D015335:Molecular Probes; D011134:Polysaccharides; C112685:fucosyl-1-2-galactose