title: [Proteomic analysis of the hematopoietic stem cell niche: focus on osteoblasts].
abstract: Methods of proteomics were used to show the influence of parathyroid hormone (PTH, fragment 1-34) on osteoblasts as a major component of the hematopoietic stem cell niche. It was found that a number of proteins are changing in response to PTH treatment for 4, 8 and 32 hours. The experimental work-flow has been optimised for the systematic identification of protein level changes upon cellular exposure to different environments.
authors.forename: N S
authors.initials: NS
authors.lastname: Zhukovskiĭ
authors.forename: V P
authors.initials: VP
authors.lastname: Karagodin
authors.forename: I A
authors.initials: IA
authors.lastname: Sobenin
mesh_terms: D045744:Cell Line, Tumor; D060833:Cellular Microenvironment / Q000187:drug effects / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D010281:Parathyroid Hormone / Q000494:pharmacology; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics
chemical_list: D010281:Parathyroid Hormone; D020543:Proteome