title: Altered branching patterns of Purkinje cells in mouse model for cortical development disorder.
abstract: Disrupted cortical cytoarchitecture in cerebellum is a typical pathology in reeler. Particularly interesting are structural problems at the cellular level: dendritic morphology has important functional implication in signal processing. Here we describe a combinatorial imaging method of synchrotron X-ray microtomography with Golgi staining, which can deliver 3-dimensional(3-D) micro-architectures of Purkinje cell(PC) dendrites, and give access to quantitative information in 3-D geometry. In reeler, we visualized in 3-D geometry the shape alterations of planar PC dendrites (i.e., abnormal 3-D arborization). Despite these alterations, the 3-D quantitative analysis of the branching patterns showed no significant changes of the 77 ± 8° branch angle, whereas the branch segment length strongly increased with large fluctuations, comparing to control. The 3-D fractal dimension of the PCs decreased from 1.723 to 1.254, indicating a significant reduction of dendritic complexity. This study provides insights into etiologies and further potential treatment options for lissencephaly and various neurodevelopmental disorders.
authors.affiliation: X-ray Imaging Center, Pohang University of Science and Technology (POSTECH), Pohang, 790-784, South Korea.
authors.forename: Jinkyung
authors.initials: J
authors.lastname: Kim
authors.forename: Namseop
authors.initials: N
authors.lastname: Kwon
authors.forename: Soeun
authors.initials: S
authors.lastname: Chang
authors.forename: Kyong-Tai
authors.initials: KT
authors.lastname: Kim
authors.forename: Dongmyeong
authors.initials: D
authors.lastname: Lee
authors.forename: Seunghwan
authors.initials: S
authors.lastname: Kim
authors.forename: So Jeong
authors.initials: SJ
authors.lastname: Yun
authors.forename: Daehee
authors.initials: D
authors.lastname: Hwang
authors.forename: Jee Woong
authors.initials: JW
authors.lastname: Kim
authors.forename: Yeukuang
authors.initials: Y
authors.lastname: Hwu
authors.forename: Giorgio
authors.initials: G
authors.lastname: Margaritondo
authors.forename: Jung Ho
authors.initials: JH
authors.lastname: Je
authors.forename: Im Joo
authors.initials: IJ
authors.lastname: Rhyu
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000000981:diagnostic imaging / Q000254:growth & development / Q000473:pathology*; D003712:Dendrites / Q000000981:diagnostic imaging / Q000473:pathology; D004195:Disease Models, Animal; D017709:Fractals; D021621:Imaging, Three-Dimensional; D054082:Lissencephaly / Q000473:pathology; D054220:Malformations of Cortical Development / Q000473:pathology; D051379:Mice; D008818:Mice, Neurologic Mutants; D011689:Purkinje Cells / Q000000981:diagnostic imaging / Q000473:pathology*; D055114:X-Ray Microtomography