title: Discovery of centrosomal RNA and centrosomal hypothesis of cellular ageing and differentiation.
abstract: In 2006, a group of scientists studying centrosomes of Spisula solidissima mollusc oocytes under the leadership of Alliegro (Alliegro, M.C.; Alliegro, M.A.; Palazzo, R.E. Centrosome-associated RNA in surf clam oocytes. Proc. Natl. Acad. Sci. USA 2006, 103(24), 9034-9038) reliably demonstrated the existence of specific RNA in centrosome, called centrosomal RNA (cnRNA). In their first article, five different RNAs (cnRNAs 11, 102, 113, 170, and 184) were described. During the process of full sequencing of the first transcript (cnRNA 11), it was discovered that the transcript contained a conserved structure-a reverse transcriptase domain located together with the most important centrosomal protein, γ-tubulin. In an article published in 2005, we made assumptions about several possible mechanisms for determining the most important functions of centrosomal structures and referred to one of them as a "RNA-dependent mechanism." This idea about participation of hypothetic centrosomal small interference RNA and/or microRNA in the process was made one year prior to the discovery of cnRNA by Alliegro's group. The discovery of specific RNA in a centrosome is indirect evidence of a centrosomal hypothesis of cellular ageing and differentiation. The presence of a reverse transcriptase domain in this type of RNA, together with its uniqueness and specificity, makes the centrosome a place of information storage and reproduction.
authors.affiliation: Life Science Research Center, Tbilisi, Georgia, USA. k.chichinadze@lifescience.org.ge
authors.forename: Konstantin
authors.initials: K
authors.lastname: Chichinadze
authors.forename: Jaba
authors.initials: J
authors.lastname: Tkemaladze
authors.forename: Ann
authors.initials: A
authors.lastname: Lazarashvili
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology*; D016922:Cellular Senescence / Q000235:genetics / Q000502:physiology*; D018385:Centrosome / Q000737:chemistry*; D008954:Models, Biological; D012313:RNA / Q000235:genetics*; D012194:RNA-Directed DNA Polymerase / Q000235:genetics; D049890:Spisula / Q000201:enzymology / Q000235:genetics*
chemical_list: D012313:RNA; D012194:RNA-Directed DNA Polymerase