title: A screening method for β-glucan hydrolase employing Trypan Blue-coupled β-glucan agar plate and β-glucan zymography.
abstract: A new screening method for β-(1,3-1,6) glucan hydrolase was developed using a pure β-glucan from Aureobaisidum pullulans by zymography and an LB-agar plate. Paenibacillus sp. was screened as a producer a β-glucan hydrolase on the Trypan Blue-coupled β-glucan LB-agar plate and the activity of the enzyme was analyzed by SDS-β-glucan zymography. The β-glucan was not hydrolyzed by Bacillus spp. strains, which exhibit cellulolytic activity on CMC zymography. The gene, obtaining by shotgun cloning and encoding the β-glucan hydrolase of Paenibacillus sp. was sequenced.
authors.affiliation: Department of Food Science & Technology, Catholic University of Daegu, Gyeongbuk, Korea.
authors.forename: Chang-Su
authors.initials: CS
authors.lastname: Park
authors.forename: Hee-Jong
authors.initials: HJ
authors.lastname: Yang
authors.forename: Dong-Ho
authors.initials: DH
authors.lastname: Kim
authors.forename: Dae-Ook
authors.initials: DO
authors.lastname: Kang
authors.forename: Min-Soo
authors.initials: MS
authors.lastname: Kim
authors.forename: Nack-Shick
authors.initials: NS
authors.lastname: Choi
mesh_terms: D000362:Agar; D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology; D002480:Cellulase / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D003001:Cloning, Molecular; D003470:Culture Media / Q000737:chemistry*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004586:Electrophoresis / Q000379:methods*; D004795:Enzyme Stability; D005936:Glucans / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008403:Mass Screening / Q000379:methods*; D008828:Microbiological Techniques / Q000379:methods*; D008969:Molecular Sequence Data; D056507:Paenibacillus / Q000201:enzymology; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013696:Temperature; D014343:Trypan Blue / Q000378:metabolism*
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial; D005936:Glucans; D000362:Agar; D002480:Cellulase; D014343:Trypan Blue