title: Visualization of dynamic change in contraction-induced lipid composition in mouse skeletal muscle by matrix-assisted laser desorption/ionization imaging mass spectrometry.
abstract: Lipids in skeletal muscle play a fundamental role both in normal muscle metabolism and in disease states. Skeletal muscle lipid accumulation is associated with several chronic metabolic disorders, including obesity, insulin resistance, and type 2 diabetes. However, it is poorly understood whether the lipid composition of skeletal muscle changes by contraction, due to the complexity of lipid molecular species. In this study, we used matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) to investigate changes in skeletal muscle lipid composition induced by contraction. We successfully observed the reduction of diacylglycerol and triacylglycerol, which are generally associated with muscle contraction. Interestingly, we found the accumulation of some saturated and mono-unsaturated fatty acids and poly-unsaturated fatty acids containing phosphatidylcholine in contracted muscles. Moreover, the distributions of several types of lipid were changed by contraction. Our results show that changes in the lipid amount, lipid composition, and energy metabolic activity can be evaluated in each local spot of cells and tissues at the same time using MALDI-IMS. In conclusion, MALDI-IMS is a powerful tool for studying lipid changes associated with contractions.
authors.affiliation: Department of Health Promotion Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, 1-1, Minami-Osawa, Hachioji, Tokyo 192-0397, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Goto-Inoue
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Manabe
authors.forename: Shouta
authors.initials: S
authors.lastname: Miyatake
authors.forename: Shinya
authors.initials: S
authors.lastname: Ogino
authors.forename: Ai
authors.initials: A
authors.lastname: Morishita
authors.forename: Takahiro
authors.initials: T
authors.lastname: Hayasaka
authors.forename: Noritaka
authors.initials: N
authors.lastname: Masaki
authors.forename: Mitsutoshi
authors.initials: M
authors.lastname: Setou
authors.forename: Nobuharu L
authors.initials: NL
authors.lastname: Fujii
mesh_terms: D000818:Animals; D008055:Lipids / Q000032:analysis* / Q000737:chemistry; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D018482:Muscle, Skeletal / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry
chemical_list: D008055:Lipids