title: Microsatellite loci for Gossypium davidsonii (Malvaceae) and other D-genome, Sonoran Desert endemic cotton species.
abstract: OBJECTIVE
  Microsatellite primers previously developed for domesticated cotton (Gossypium hirsutum; tetraploid) were screened for their utility in investigating genetic structure and gene flow within G. davidsonii and five other wild, Mexican, D-genome cotton species (all diploid).
  
  
  RESULTS
  We screened 50 microsatellite primer pairs from the Cotton Marker Database, identifying 10 loci as polymorphic within G. davidsonii. In genotyping approximately 200 individuals from four populations, we found that the number of alleles per locus ranged from seven to 17, and mean observed and expected heterozygosity ranged from 0.145 to 0.492 and from 0.436 to 0.734, respectively. We genotyped six to 20 individuals from each of the remaining species, finding these 10 loci to cross-amplify in all cases and in most cases to be polymorphic.
  
  
  CONCLUSIONS
  These markers may be useful for further investigation of population genetics of G. davidsonii and other wild D-genome cotton species.
authors.affiliation: Department of Ecology, Evolution, and Organismal Biology, Iowa State University, Ames, Iowa 50011 USA.
authors.forename: Adam P
authors.initials: AP
authors.lastname: Kuester
authors.forename: John D
authors.initials: JD
authors.lastname: Nason
mesh_terms: D003710:Demography; D003889:Desert Climate; D018745:Genome, Plant; D005838:Genotype; D003368:Gossypium / Q000235:genetics*; D008800:Mexico; D018895:Microsatellite Repeats / Q000235:genetics*; D013045:Species Specificity