title: New applications of alphavirus-based expression vectors.
abstract: Alphaviruses are positive stranded RNA viruses that replicate to extremely high titers. Sindbis and Semliki Forest viral vectors are widely used tools for high-level production of recombinant proteins. Recent studies have broadened their scope to vaccine production, gene therapy, and analysis of cell function. Here we discuss the development of non-cytopathic and inducible expression vectors which can be applied to bioprocess development strategies. Furthermore, a Sindbis-based expression cloning system has been developed that allows for the rapid identification of genes encoding proteins with a selected functional activity.
authors.affiliation: Cytos Biotechnology AG, Wagistrasse 21, CH-8952, Zurich-Schlieren, Switzerland.
authors.forename: M
authors.initials: M
authors.lastname: Boorsma
authors.forename: D
authors.initials: D
authors.lastname: Koller
authors.forename: W A
authors.initials: WA
authors.lastname: Renner
authors.forename: M F
authors.initials: MF
authors.lastname: Bachmann