title: A protocol for transformation of Torenia.
abstract: This chapter describes an Agrobacterium tumefaciens-mediated transformation protocol for torenia, a plant that has several useful characteristics and is primarily used for ornamental and experimental purposes. Leaf segments of torenia were co-cultured with A. tumefaciens containing a vector plasmid for 7 days at 22°C under dark conditions on Murashige and Skoog (MS) medium containing 1 mg/L benzyladenine, 1 mg/L indoleacetic acid, and 100 μM acetosyringone. Subsequent culturing at 25°C under a 16-h photoperiod with fluorescent light on MS medium containing 1 mg/L benzyladenine, 300 mg/L carbenicillin, and selection agent (300 mg/L kanamycin or 20 mg/L hygromycin) allowed for transformant selection. Transgenic shoots were obtained from green compact calli after 2-3 months of culture in the selection medium. This method can achieve a transformation rate of approximately 5% (transformants/explant).
authors.affiliation: National Institute of Floricultural Science, National Agriculture and Food Research Organization, Tsukuba, Ibaraki, Japan. ryu@affrc.go.jp
authors.forename: Ryutaro
authors.initials: R
authors.lastname: Aida
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D007612:Kanamycin / Q000494:pharmacology; D007613:Kanamycin Resistance / Q000235:genetics; D030821:Plants, Genetically Modified; D010957:Plasmids / Q000235:genetics; D028083:Scrophulariaceae / Q000235:genetics*; D046509:Tissue Culture Techniques; D014170:Transformation, Genetic
chemical_list: D007612:Kanamycin