title: The usefulness of CHAPS as a non-cytotoxic stabilizing agent in purification of growth factors.
abstract: Among several detergents, a zwitterionic detergent, 3-[(3-cholamidopropyl)dimethylammonio]-1-propane sulfonate (CHAPS), was found to be least cytotoxic for cultured mammalian cells. CHAPS improved the activity recovery and elution profile of crude and purified fibroblast growth factors (FGFs) during chromatographies. Diluted preparations of FGFs were stabilized by CHAPS against the loss during storage. Amino acid sequence analysis was not disturbed by CHAPS. CHAPS was removable by reversed-phase high-performance liquid chromatography. These results indicate that CHAPS is useful as a non-cytotoxic stabilizing agent in purification of various kinds of bioactive polypeptides.
authors.affiliation: Department of Endocrinology, Kagawa Medical School, 1750, Miki-cho, Kita-gun, 761-07, Kagawa, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Matuo
authors.forename: N
authors.initials: N
authors.lastname: Nishi
authors.forename: Y
authors.initials: Y
authors.lastname: Muguruma
authors.forename: Y
authors.initials: Y
authors.lastname: Yoshitake
authors.forename: Y
authors.initials: Y
authors.lastname: Masuda
authors.forename: K
authors.initials: K
authors.lastname: Nishikawa
authors.forename: F
authors.initials: F
authors.lastname: Wada