title: The earliest thymic T cell progenitors sustain B cell and myeloid lineage potential.
abstract: The stepwise commitment from hematopoietic stem cells in the bone marrow to T lymphocyte-restricted progenitors in the thymus represents a paradigm for understanding the requirement for distinct extrinsic cues during different stages of lineage restriction from multipotent to lineage-restricted progenitors. However, the commitment stage at which progenitors migrate from the bone marrow to the thymus remains unclear. Here we provide functional and molecular evidence at the single-cell level that the earliest progenitors in the neonatal thymus had combined granulocyte-monocyte, T lymphocyte and B lymphocyte lineage potential but not megakaryocyte-erythroid lineage potential. These potentials were identical to those of candidate thymus-seeding progenitors in the bone marrow, which were closely related at the molecular level. Our findings establish the distinct lineage-restriction stage at which the T cell lineage-commitment process transits from the bone marrow to the remote thymus.
authors.affiliation: Haematopoietic Stem Cell Laboratory, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford, Oxford, UK.
authors.forename: Sidinh
authors.initials: S
authors.lastname: Luc
authors.forename: Tiago C
authors.initials: TC
authors.lastname: Luis
authors.forename: Hanane
authors.initials: H
authors.lastname: Boukarabila
authors.forename: Iain C
authors.initials: IC
authors.lastname: Macaulay
authors.forename: Natalija
authors.initials: N
authors.lastname: Buza-Vidas
authors.forename: Tiphaine
authors.initials: T
authors.lastname: Bouriez-Jones
authors.forename: Michael
authors.initials: M
authors.lastname: Lutteropp
authors.forename: Petter S
authors.initials: PS
authors.lastname: Woll
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Loughran
authors.forename: Adam J
authors.initials: AJ
authors.lastname: Mead
authors.forename: Anne
authors.initials: A
authors.lastname: Hultquist
authors.forename: John
authors.initials: J
authors.lastname: Brown
authors.forename: Takuo
authors.initials: T
authors.lastname: Mizukami
authors.forename: Sahoko
authors.initials: S
authors.lastname: Matsuoka
authors.forename: Helen
authors.initials: H
authors.lastname: Ferry
authors.forename: Kristina
authors.initials: K
authors.lastname: Anderson
authors.forename: Sara
authors.initials: S
authors.lastname: Duarte
authors.forename: Deborah
authors.initials: D
authors.lastname: Atkinson
authors.forename: Shamit
authors.initials: S
authors.lastname: Soneji
authors.forename: Aniela
authors.initials: A
authors.lastname: Domanski
authors.forename: Alison
authors.initials: A
authors.lastname: Farley
authors.forename: Alejandra
authors.initials: A
authors.lastname: Sanjuan-Pla
authors.forename: Cintia
authors.initials: C
authors.lastname: Carella
authors.forename: Roger
authors.initials: R
authors.lastname: Patient
authors.forename: Marella
authors.initials: M
authors.lastname: de Bruijn
authors.forename: Tariq
authors.initials: T
authors.lastname: Enver
authors.forename: Claus
authors.initials: C
authors.lastname: Nerlov
authors.forename: Clare
authors.initials: C
authors.lastname: Blackburn
authors.forename: Isabelle
authors.initials: I
authors.lastname: Godin
authors.forename: Sten Eirik W
authors.initials: SE
authors.lastname: Jacobsen
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology*; D019070:Cell Lineage / Q000276:immunology*; D002469:Cell Separation; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology; D054503:Lymphoid Progenitor Cells / Q000166:cytology* / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D022423:Myeloid Cells / Q000166:cytology*; D020411:Oligonucleotide Array Sequence Analysis; D054448:Precursor Cells, B-Lymphoid / Q000166:cytology*; D060888:Real-Time Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000166:cytology*; D013950:Thymus Gland / Q000166:cytology