title: [Changes in the expression of Dlx5, Msx2, and Dlx5/Msx2 in hPDLSCs loaded with cyclic tensile stress].
abstract: OBJECTIVE
  To investigate the changes in the expression of Dlx5 and Msx2 in human periodontal ligament stem cells (hPDLSCs) loaded with cyclic tensile stress.
  
  
  METHODS
  hPDLSCs were subjected to cyclic tensile stress (0.5 Hz, 3000 microm strain) for 3, 6, 12, 24 h through a four-point bending strain system. The expressions of Dlx5 and Msx2 mRNA were determined by real time PCR.
  
  
  RESULTS
  Strong expressions of Dlx5 and Msx2 were found in the periodontal fibroblasts of the tension side 20 minutes after mechanical loading. The expression of Dlx5 mRNA decreased over time with the stress. The expression of Msx2 mRNA increased over time with the stress.
  
  
  CONCLUSIONS
  Both Dlx5 and Msx2 are sensitive to mechanical stress. Cyclic tensile stress may induce differentiating of hPDLSCs towards mineralized tissue cells by promoting Dlx5 mRNA expression and decreasing Msx2 expression.
authors.affiliation: Department of Orthodontics, West China Stomatological Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Chuan-xi
authors.initials: CX
authors.lastname: Sun
authors.forename: Lin-kun
authors.initials: LK
authors.lastname: Zhang
authors.forename: Xiao-xia
authors.initials: XX
authors.lastname: Feng
authors.forename: Zhi-he
authors.initials: ZH
authors.lastname: Zhao
mesh_terms: D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D010006:Osteoblasts / Q000166:cytology; D010507:Periodicity; D010513:Periodontal Ligament / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D013314:Stress, Mechanical; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: C100596:DLX5 protein, human; D018398:Homeodomain Proteins; C084119:MSX2 protein; D014157:Transcription Factors