title: Simultaneous detection of residues of 25 β₂-agonists and 23 β-blockers in animal foods by high-performance liquid chromatography coupled with linear ion trap mass spectrometry.
abstract: A sensitive method has been developed for the simultaneous determination of residues of 25 β₂-agonists and 23 β-blockers in animal foods by high-performance liquid chromatography coupled with linear ion trap mass spectrometry (HPLC-LIT-MS). This method is based on a new procedure of hydrolysis and extraction by 5% trichloracetic acid, and then cleaned up by mixed strong cation exchange (MCX) cartridges coupled with a novelty cleanup step by methanol. Methanol and 0.1% formic acid were used as mobile phases for gradient elution, while a Supelco Ascentis Express Rp-Amide column was used for LC separation. ESI positive ion scan mode was used with consecutive reaction monitoring (CRM, MS³). Nine β₂-agonists labeled by the deuterium isotope were used as internal standards for quantification. The linear ranges of 48 analytes were from 5 to 200 μg/L; the coefficient of correlation was not less than 0.995. Blank pork muscle, blank liver, and blank kidney were selected as representative matrix for spiked standard recovery test. The recoveries of each compound were in the range of 46.6-118.9%, and the relative standard deviations were in the range of 1.9-28.2%. Decision limits (CCα, α = 0.01) of 48 analytes in muscles, liver, and kidney samples ranged from 0.05 to 0.49 μg/kg, and the detection capability (CCβ, β = 0.05) ranged from 0.13 to 1.64 μg/kg. This method was successfully applied to 110 real animal origin food samples including meat, liver, and kidney of pig and chicken samples.
authors.affiliation: Institute of Nutrition and Food Hygiene, Beijing Centre for Disease Control and Prevention, Beijing 100013, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Sai
authors.forename: Miao
authors.initials: M
authors.lastname: Hong
authors.forename: Zhao
authors.initials: Z
authors.lastname: Yunfeng
authors.forename: Chen
authors.initials: C
authors.lastname: Huijing
authors.forename: Wu
authors.initials: W
authors.lastname: Yongning
mesh_terms: D058666:Adrenergic beta-2 Receptor Agonists / Q000032:analysis* / Q000737:chemistry; D000319:Adrenergic beta-Antagonists / Q000008:administration & dosage* / Q000737:chemistry; D000818:Animals; D002417:Cattle; D002645:Chickens; D002681:China; D002851:Chromatography, High Pressure Liquid; D004350:Drug Residues / Q000032:analysis* / Q000737:chemistry; D005506:Food Contamination; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008460:Meat / Q000032:analysis*; D008461:Meat Products / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization; D034421:Sus scrofa; D053719:Tandem Mass Spectrometry
chemical_list: D058666:Adrenergic beta-2 Receptor Agonists; D000319:Adrenergic beta-Antagonists