title: Prolonged increase in rat hippocampal chemokine signalling after status epilepticus.
abstract: Temporal lobe epilepsy (TLE) is one of the most common focal epilepsy syndromes. In a genome-wide expression study of the human TLE hippocampus we previously showed up-regulation of genes involved in chemokine signalling. Here we investigate in the rat pilocarpine model for TLE, whether changes in chemokine signalling occur during epileptogenesis and are persistent. Therefore we analysed hippocampal protein expression and cellular localisation of CCL2, CCL4, CCR1 and CCR5 after status epilepticus. We found increased CCL4 (but not CCL2) expression in specific populations of hilar astrocytes at 2 and 19 weeks after SE concomitant with a persistent up-regulation of its receptor CCR5. Our results show an early and persistent up-regulation of CCL4/CCR5 signalling during epileptogenesis and suggest that CCL4 signalling, rather than CCL2 signalling, could have a role in the epileptogenic process.
authors.affiliation: Dept. of Neuroscience & Pharmacology, Rudolf Magnus Institute of Neuroscience, The Netherlands.
authors.forename: Anne A
authors.initials: AA
authors.lastname: Kan
authors.forename: W Saskia
authors.initials: WS
authors.lastname: van der Hel
authors.forename: Sharon M
authors.initials: SM
authors.lastname: Kolk
authors.forename: Ineke W M
authors.initials: IW
authors.lastname: Bos
authors.forename: Suzanne A M W
authors.initials: SA
authors.lastname: Verlinde
authors.forename: Onno
authors.initials: O
authors.lastname: van Nieuwenhuizen
authors.forename: Pierre N E
authors.initials: PN
authors.lastname: de Graan
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000276:immunology / Q000378:metabolism; D054407:Chemokine CCL4 / Q000378:metabolism*; D004195:Disease Models, Animal; D004833:Epilepsy, Temporal Lobe / Q000276:immunology* / Q000378:metabolism; D006624:Hippocampus / Q000276:immunology* / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D011930:Reaction Time / Q000276:immunology; D019713:Receptors, CCR5 / Q000378:metabolism*; D015398:Signal Transduction / Q000276:immunology*; D013226:Status Epilepticus / Q000276:immunology* / Q000378:metabolism; D015854:Up-Regulation / Q000276:immunology
chemical_list: D054407:Chemokine CCL4; D019713:Receptors, CCR5