title: Atmospheric pressure photoionization as a powerful tool for large-scale lipidomic studies.
abstract: Lipidomic studies often use liquid chromatography/electrospray ionization mass spectrometry (LC/ESI-MS) for separation, identification, and quantification. However, due to the wide structural diversity of lipids, the most apolar part of the lipidome is often detected with low sensitivity in ESI. Atmospheric pressure (APPI) can be an alternative ionization source since normal-phase solvents are known to enhance photoionization of these classes. In this paper, we intend to show the efficiency of APPI to identify different lipid classes, with a special interest on sphingolipids. In-source APPI fragmentation appears to be an added value for the structural analysis of lipids. It provides a detailed characterization of both the polar head and the non polar moiety of most lipid classes, and it makes possible the detection of all lipids in both polarities, which is not always possible with ESI.
authors.affiliation: Centre de Recherche de Gif, Institut de Chimie des Substances Naturelles, CNRS, Gif-sur-Yvette, France.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Gaudin
authors.forename: Laurent
authors.initials: L
authors.lastname: Imbert
authors.forename: Danielle
authors.initials: D
authors.lastname: Libong
authors.forename: Pierre
authors.initials: P
authors.lastname: Chaminade
authors.forename: Alain
authors.initials: A
authors.lastname: Brunelle
authors.forename: David
authors.initials: D
authors.lastname: Touboul
authors.forename: Olivier
authors.initials: O
authors.lastname: Laprévote
mesh_terms: D001274:Atmospheric Pressure; D019295:Computational Biology / Q000379:methods*; D008055:Lipids / Q000032:analysis*; D010777:Photochemistry / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014315:Triterpenes / Q000032:analysis
chemical_list: D008055:Lipids; D014315:Triterpenes