title: Amino acid metabolism during batch culture of a murine hybridoma, AFP-27.
abstract: This paper presents batch culture data of the murine hybridoma, AFP-27, cultured in conventional basal media and in a nutrient-rich modified version. Expression of antibody was fivefold higher in the enriched formulation, with significant product secretion in the decline phase. Cultures were initiated at conventional inculation densities (1 ∼ 2 × 10(5) viable cells ml(-1)) and high inoculation densities (1.5 ∼ 1.7 × 10(6) viable cells ml(-1)). Amino acid levels have been reported for all cultures, with apparent differences described. Relative levels of intracellular amino acids are also reported, with significant accumulation of proline, glycine and alanine. The results have significance in the design of enriched media which are clearly beneficial for commercial production of antibodies from hybridomas.
authors.affiliation: Department of Biotechnology, University of NSW, 2052, NSW, Australia.
authors.forename: C P
authors.initials: CP
authors.lastname: Marquis
authors.forename: J P
authors.initials: JP
authors.lastname: Barford
authors.forename: C
authors.initials: C
authors.lastname: Harbour