title: Cerebral endothelial derived vascular endothelial growth factor promotes the migration but not the proliferation of oligodendrocyte precursor cells in vitro.
abstract: In gray matter, cerebral endothelium is known to provide trophic support for neighboring cells such as neurons. However, signaling from cerebral endothelium to white matter cells remains to be elucidated. Here, we show that vascular endothelial growth factor (VEGF-A) secreted from cerebral endothelial cells promotes the migration but not the proliferation of oligodendrocyte precursor cells (OPCs). Cultured OPCs were obtained from newborn rat cortex, and treatment with conditioned culture media of cerebral endothelial cells increased the OPC proliferation and migration. Importantly, co-treatment with anti-neutralizing antibody for Flk-1 (VEGF-receptor2) inhibited OPC movement but did not affect OPC propagation. Western blot and flow cytometry analyses confirmed that our cultured cerebral endothelial cells produced VEGF-A and our cultured OPCs expressed Flk-1. Taken together, our current data suggest that cerebral endothelium is supportive for oligodendrocyte lineage cells and VEGF-A may participate in the endothelium-OPC cell-cell signaling. This phenomenon may be important for white matter homeostasis.
authors.affiliation: Neuroprotection Research Laboratory, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA 02129, USA.
authors.forename: Kazuhide
authors.initials: K
authors.lastname: Hayakawa
authors.forename: Ji Hae
authors.initials: JH
authors.lastname: Seo
authors.forename: Loc-Duyen D
authors.initials: LD
authors.lastname: Pham
authors.forename: Nobukazu
authors.initials: N
authors.lastname: Miyamoto
authors.forename: Angel T
authors.initials: AT
authors.lastname: Som
authors.forename: Shuzhen
authors.initials: S
authors.lastname: Guo
authors.forename: Kyu-Won
authors.initials: KW
authors.lastname: Kim
authors.forename: Eng H
authors.initials: EH
authors.lastname: Lo
authors.forename: Ken
authors.initials: K
authors.lastname: Arai
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D017077:Culture Media, Conditioned; D042783:Endothelial Cells / Q000187:drug effects; D005434:Flow Cytometry; D007150:Immunohistochemistry; D058953:Neural Stem Cells / Q000187:drug effects*; D009836:Oligodendroglia / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D040262:Receptors, Vascular Endothelial Growth Factor / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology*; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000494:pharmacology
chemical_list: D015415:Biomarkers; D017077:Culture Media, Conditioned; D042461:Vascular Endothelial Growth Factor A; D040262:Receptors, Vascular Endothelial Growth Factor; D040301:Vascular Endothelial Growth Factor Receptor-2