title: [Expression of enoyl CoA hydratase 1 reduces cell proliferation and migration in mouse hepatocarcinoma cells].
abstract: OBJECTIVE
  To study the expression of enoyl CoA hydratase 1 (ECH1) and the effect when down-regulation of ECH1 gene expression in mouse hepatocarcinoma cell.
  
  
  METHODS
  Immunofluorescence was used for detecting the expression of ECH1, and stably transfected Hca-F cells with pGPU6/GFP/Neo-shRNA-ECH1 expression plasmids. Cell proliferation was assessed by Cell counting kit-8 (CCK8) assay. The Boyden-transwell assay (8 µm pore size) was performed to analyze the inhibitory effect of shRNA on Hca-F cell migration and invasion.
  
  
  RESULTS
  ECH1 expression was obtained in the cytoplasm and upregulated expression in Hca-F cells than that in Hca-P cells. The down-regulation of ECH1 could inhibit the cell proliferation of Hca-F cells, decrease the number of cell pass through Transwell (27.07 ± 17.49) compared with scramble-negative (72.38 ± 18.83) and Hca-F controls (59.06 ± 30.33), decrease the migration capacities of Hca-F cells, increase the ratio of Hca-F cells in S phase (86.1%) compared with scramble-negative (75.8%) and Hca-F controls (66.2%) and decrease the ratio of G(1) phase (9.4%) compared with scramble-negative (24.2%) and Hca-F controls (30.3%).
  
  
  CONCLUSIONS
  ECH1 serves as a potential critical factor attributes to tumor lymphatic metastasis.
authors.affiliation: Department of Pathology, Dalian Medical University, Dalian, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Jian-wu
authors.initials: JW
authors.lastname: Tang
authors.forename: Mei-ying
authors.initials: MY
authors.lastname: Song
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.forename: Rong-kuan
authors.initials: RK
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Hou
authors.forename: Yu-hong
authors.initials: YH
authors.lastname: Huang
authors.forename: Mei
authors.initials: M
authors.lastname: Wang
authors.forename: Bo
authors.initials: B
authors.lastname: Song
authors.forename: Yan-Ling
authors.initials: YL
authors.lastname: Jin
mesh_terms: D000818:Animals; D002453:Cell Cycle; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D003593:Cytoplasm / Q000201:enzymology; D015536:Down-Regulation; D004746:Enoyl-CoA Hydratase / Q000235:genetics / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D008114:Liver Neoplasms, Experimental / Q000201:enzymology / Q000473:pathology*; D008207:Lymphatic Metastasis; D051379:Mice; D010957:Plasmids; D034741:RNA, Small Interfering / Q000235:genetics*; D014162:Transfection
chemical_list: D034741:RNA, Small Interfering; D004746:Enoyl-CoA Hydratase