title: [Rhesus monkey embryonic stem cells differentiation, proliferation and allotransplantation].
abstract: To investigate the characteristics of rhesus monkey embryonic stem cells and to promote their clinical application, the differentiation and proliferation of rosettes neural stem cells from GFP marked rhesus monkey embryonic stem cells were studied The results showed that: 1) A stable and high-efficient neural differentiation system was established. More than 95% of the embryonic stem cells were differentiated into neural stem cells on the 12(th) days of differentiation; 2) the rosettes neural stem cells differentiated from the rhesus monkey embryonic stem cells could maintain their rosettes-shape by proliferating with bFGF/EGF; 3) the neural stem cells could differentiate into neurons after transplanted into the rhesus monkey brain. In conclusion, the rosettes neural stem cells differentiated from rhesus monkey embryonic stem cells could maintain their characteristics after proliferation with bFGF/EGF and they could survive and differentiate into neurons after transplanted into the rhesus monkey brain, which strongly supports the clinical application of neural stem cells in the future.
authors.affiliation: Institute of Biophysics, the Chinese Academy of Sciences, Beijing, China.
authors.forename: Jin-Run
authors.initials: JR
authors.lastname: Dong
authors.forename: Li-Yun
authors.initials: LY
authors.lastname: Guo
authors.forename: Jia-Gui
authors.initials: JG
authors.lastname: Qu
authors.forename: Ren-Li
authors.initials: RL
authors.lastname: Qi
authors.forename: Wen-Chao
authors.initials: WC
authors.lastname: Wang
authors.forename: Chun-Jie
authors.initials: CJ
authors.lastname: Xiao
authors.forename: Zheng-Bo
authors.initials: ZB
authors.lastname: Wang
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D004195:Disease Models, Animal; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000637:transplantation*; D006801:Humans; D008253:Macaca mulatta / Q000502:physiology; D009420:Nervous System / Q000166:cytology; D009422:Nervous System Diseases / Q000601:surgery / Q000628:therapy*; D009474:Neurons / Q000166:cytology; D014184:Transplantation, Homologous