title: Detection of small bioactive peptides from Atlantic herring (Clupea harengus L.).
abstract: Recent research has shown that fish residual materials contain a range of components with interesting biological activity. Therefore, there is a great potential in the marine bioprocess industry to utilize these by-products as starting material for generating more valuable products. The aim of the present study was to search for bioactive peptides (in particular small natural bioactive peptides with molecular weight lower than 10 kDa) in Atlantic herring (Clupea harengus L.) by-products such as skin and more general residual materials. By such means a range of peptides with claimed interesting biological activities was found. Herein the activity of the detected bioactive peptides and strategies for isolating peptide fragments containing the bioactive motif is discussed. Identification of bioactive peptides in crude peptide/protein sources (skin and residual materials) was performed directly using a combination of mass spectrometry (Orbitrap), bioinformatics and database search. This method was a good angle of approach in order to map the potential in new species and species that have been very little studied.
authors.affiliation: Biomiljø, International Research Institute of Stavanger, Mekjarvik 12, NO-4070 Randaberg, Norway.
authors.forename: Daniela M
authors.initials: DM
authors.lastname: Pampanin
authors.forename: Eivind
authors.initials: E
authors.lastname: Larssen
authors.forename: Fiona
authors.initials: F
authors.lastname: Provan
authors.forename: Morten
authors.initials: M
authors.lastname: Sivertsvik
authors.forename: Peter
authors.initials: P
authors.lastname: Ruoff
authors.forename: Magne O
authors.initials: MO
authors.lastname: Sydnes
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D001685:Biological Factors / Q000737:chemistry / Q000302:isolation & purification*; D019295:Computational Biology; D016208:Databases, Factual; D005399:Fishes / Q000378:metabolism*; D013058:Mass Spectrometry; D009842:Oligopeptides / Q000737:chemistry / Q000302:isolation & purification*; D012867:Skin / Q000737:chemistry*
chemical_list: D001685:Biological Factors; D009842:Oligopeptides