title: Elevated serum antibodies against insulin-like growth factor-binding protein-2 allow detecting early-stage cancers: evidences from glioma and colorectal carcinoma studies.
abstract: BACKGROUND
  Tumor-specific immunity of insulin-like growth factor-binding protein-2 (IGFBP-2) has been reported in several cancers. We aimed to assess the role of serum IGFBP-2 antibodies (IGFBP-2 Abs) in early cancer detection.
  
  
  METHODS
  Glioma and colorectal carcinoma (CRC) were used as models. Serum IGFBP-2 and IGFBP-2 Abs were measured in 260 tumor patients (145 gliomas, 45 colorectal polyps, and 70 CRCs) and 141 controls. Receiver operating characteristic curves were applied.
  
  
  RESULTS
  Serum IGFBP-2 Ab levels were significantly elevated in tumors (mean: 82 ng/ml, median: 17 ng/ml, range: 0-1387 ng/ml) compared with controls (11, 0, 0-212 ng/ml) (P < 0.0001) and higher in early than advanced cancers opposite of serum IGFBP-2 levels. IGFBP-2 Abs effectively discriminated between controls and grade II and III gliomas [area under the curve (AUC): 0.821-0.864; 95% confidence interval (CI) = 0.762-0.936; P < 0.0001], and CRC I-II (AUC: 0.668; 95% CI = 0.566-0.770; P = 0.002) as well as indicative of advanced polyps at high risk of CRC (AUC: 0.72; 95% CI = 0.630-0.811; P < 0.0001). The sensitivity and specificity for diagnosing grade II-III gliomas reached 66%-84% and 81%. Combined serum IGFBP-2 and IGFBP-2 Abs augmented the discriminative power of all stage tumors (AUC: 0.823), gliomas (AUC: 0.800), and CRCs (AUC = 0.917).
  
  
  CONCLUSIONS
  Our results first demonstrate IGFBP-2 Abs for early cancer detection and in combination of serum IGFBP-2 for improved cancer diagnosis.
authors.affiliation: Department of Oncology, Beijing Shijitan Hospital, The Capital Medical University, Beijing.
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, Tiantan Hospital, Capital Medical University, Beijing.
authors.forename: T
authors.initials: T
authors.lastname: Jiang
authors.affiliation: Laboratory of Disease Genomics and individualized Medicine, Center of Computational Biology, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing.
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key laboratory of Carcinogenesis and Translational Research (Ministry of Education), Clinical Research Laboratory, Peking University School of Oncology, Beijing Cancer Hospital & Institute, Beijing, China.
authors.forename: B
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Key laboratory of Carcinogenesis and Translational Research (Ministry of Education), Clinical Research Laboratory, Peking University School of Oncology, Beijing Cancer Hospital & Institute, Beijing, China.
authors.forename: W
authors.initials: W
authors.lastname: Yang
authors.affiliation: Department of Neurosurgery, Tiantan Hospital, Capital Medical University, Beijing.
authors.forename: G
authors.initials: G
authors.lastname: You
authors.affiliation: Department of Oncology, Beijing Shijitan Hospital, The Capital Medical University, Beijing.
authors.forename: K
authors.initials: K
authors.lastname: Xu
authors.affiliation: Department of Oncology, Beijing Shijitan Hospital, The Capital Medical University, Beijing.
authors.forename: J
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Oncology, Beijing Shijitan Hospital, The Capital Medical University, Beijing.
authors.forename: C
authors.initials: C
authors.lastname: Luo
authors.affiliation: Key laboratory of Carcinogenesis and Translational Research (Ministry of Education), Clinical Research Laboratory, Peking University School of Oncology, Beijing Cancer Hospital & Institute, Beijing, China. Electronic address: weisonyasong@bjcancer.org.
authors.forename: S W
authors.initials: SW
authors.lastname: Song
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019540:Area Under Curve; D001254:Astrocytoma / Q000097:blood* / Q000175:diagnosis / Q000378:metabolism; D001323:Autoantibodies / Q000097:blood*; D002277:Carcinoma / Q000097:blood* / Q000175:diagnosis / Q000378:metabolism; D016022:Case-Control Studies; D016543:Central Nervous System Neoplasms / Q000097:blood* / Q000175:diagnosis / Q000378:metabolism; D015179:Colorectal Neoplasms / Q000097:blood* / Q000175:diagnosis / Q000378:metabolism; D055088:Early Detection of Cancer; D005260:Female; D006801:Humans; D018971:Insulin-Like Growth Factor Binding Protein 2 / Q000097:blood / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D012372:ROC Curve; D018709:Statistics, Nonparametric; D055815:Young Adult
chemical_list: D001323:Autoantibodies; D018971:Insulin-Like Growth Factor Binding Protein 2