title: Raising polyclonal antibodies using nitrocellulose-bound antigen.
abstract: Highly specific antibodies directed against minor proteins, present in small amounts in biological fluids, or against nonsoluble cytoplasmic or membraneous proteins, are often difficult to obtain. The main reasons for this are the small amounts of protein available after the various classical purification processes and the low purity of the proteins.
authors.affiliation: Biohgie de la Differenciation Cellulaire, Faculte des Sciences de Luminy, Marseilles, France.
authors.forename: M
authors.initials: M
authors.lastname: Diano
authors.forename: A L
authors.initials: AL
authors.lastname: Bivic
authors.forename: M
authors.initials: M
authors.lastname: Hirn