title: Experimental chronic jet lag promotes growth and lung metastasis of             Lewis lung carcinoma in C57BL/6 mice.
abstract: Circadian rhythm has been linked to cancer genesis and development, but             the detailed mechanism by which circadian disruption accelerates tumor growth             remains unclear. The purpose of this study was to investigate the effect of circadian             disruption on tumor growth and metastasis in male C57BL/6 mice, using an experimental             chronic jet lag model. Lewis lung carcinoma cells were inoculated into both flanks             of the mice following 10 days of exposure to experimental chronic jet lag or control             conditions. The effects on tumor growth and lung metastasis were assessed, and             the effect on gene expression was detected using cDNA microarrays and real-time             quantitative RT-PCR. Tumors grew faster in the experimental chronic jet lag mice             compared to the control mice (P=0.004). Lung metastases were found in 10 out of             24 mice in the chronic jet lag group, but only in 3 out of 24 mice in the LD group             (P=0.023). Microarray data showed that in both liver and tumors circadian disruption             altered the expression of genes, including those related to the cell cycle, apoptosis,             the immune response and metastasis suppressor genes. The expression of the Ndrg1             gene was suppressed by chronic jet lag. We conclude that circadian disruption             can promote tumor progression and metastasis by affecting the expression of both             tumor-related genes and metastasis suppressor genes.
authors.affiliation: State Key Laboratory of Oncology in South China, Department of Research, Cancer Center, Sun Yat-Sen University, Guangzhou, Guangdong, PR China. wumw@mail.sysu.edu.cn
authors.forename: Mingwei
authors.initials: M
authors.lastname: Wu
authors.forename: Jing
authors.initials: J
authors.lastname: Zeng
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Chen
authors.forename: Zhaolei
authors.initials: Z
authors.lastname: Zeng
authors.forename: Jinxin
authors.initials: J
authors.lastname: Zhang
authors.forename: Yuchen
authors.initials: Y
authors.lastname: Cai
authors.forename: Yanli
authors.initials: Y
authors.lastname: Ye
authors.forename: Liwu
authors.initials: L
authors.lastname: Fu
authors.forename: Lijian
authors.initials: L
authors.lastname: Xian
authors.forename: Zhongping
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000818:Animals; D001831:Body Temperature; D018827:Carcinoma, Lewis Lung / Q000235:genetics* / Q000473:pathology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D020179:Jet Lag Syndrome / Q000235:genetics*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009362:Neoplasm Metastasis