title: A more accurate profile of Achyrocline satureioides hypocholesterolemic activity.
abstract: The aim of this study was to investigate the effect of the aqueous extract (AE) of Achyrocline satureioides on serum lipid profile, liver oxidative profile and Na(+),K(+)-ATPase activity of rats submitted to a hyperlipidic diet. The animals were divided into four groups: control (C), AE 10% (A(10)), hyperlipidic (H) and hyperlipidic/AE 10% (HA(10)). In serum, we measured the levels of total cholesterol (TC), high-density lipoprotein, very-low-density lipoprotein, low-density lipoprotein (LDL) and triglyceride (TG). In liver homogenates, we measured the thiobarbituric acid reactive substances, the carbonyl proteins, the non-protein thiols (NPSHs) and the activity of superoxide dismutase, catalase (CAT) and Na(+),K(+)-ATPase. We observed a significant increase in the TC and LDL levels in the H group. A. satureioides prevented these effects, decreased the TG levels in the HA(10) group and increased the NPSH levels in the A(10) and HA(10) groups. The H group showed an increase in the carbonyl protein level and a decrease in CAT and Na(+),K(+)-ATPase activities. With the use of this model, results show that increased levels of lipids are related to a redox imbalance in the liver, which is also related to the inhibition of Na(+),K(+)-ATPase activity, and that chronic administration of the AE of A. satureioides is capable of changing this profile.
authors.affiliation: Curso de Ciências Biológicas, Centro de Ciências da Saúde, Laboratório de Biologia Molecular, Universidade de Cruz Alta, Cruz Alta, RS, Brazil.
authors.forename: Débora Corrêa
authors.initials: DC
authors.lastname: Espiña
authors.forename: Fabiano Barbosa
authors.initials: FB
authors.lastname: Carvalho
authors.forename: Daniela
authors.initials: D
authors.lastname: Zanini
authors.forename: Josiane Bizzi
authors.initials: JB
authors.lastname: Schlemmer
authors.forename: Juliane Dors
authors.initials: JD
authors.lastname: Coracini
authors.forename: Maribel Antonello
authors.initials: MA
authors.lastname: Rubin
authors.forename: Vera Maria
authors.initials: VM
authors.lastname: Morsch
authors.forename: Maria Rosa Chitolina
authors.initials: MR
authors.lastname: Schetinger
authors.forename: Daniela Bitencourt Rosa
authors.initials: DB
authors.lastname: Leal
authors.forename: Cléia Rosani
authors.initials: CR
authors.lastname: Baiotto
authors.forename: Jeandre Augusto dos Santos
authors.initials: JA
authors.lastname: Jaques
mesh_terms: D036921:Achyrocline / Q000737:chemistry*; D000818:Animals; D000924:Anticholesteremic Agents / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D002784:Cholesterol / Q000097:blood; D059305:Diet, High-Fat; D004789:Enzyme Activation / Q000187:drug effects; D008055:Lipids / Q000097:blood; D008075:Lipoproteins, HDL / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D014280:Triglycerides / Q000097:blood
chemical_list: D000924:Anticholesteremic Agents; D008055:Lipids; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D010936:Plant Extracts; D017392:Thiobarbituric Acid Reactive Substances; D014280:Triglycerides; D002784:Cholesterol; D002374:Catalase; D013482:Superoxide Dismutase; D000254:Sodium-Potassium-Exchanging ATPase