title: The mRNA expression profile of cytokines connected to the regulation of melanocyte functioning in vitiligo skin biopsy samples and peripheral blood mononuclear cells.
abstract: The expression pattern of several genes associated with different processes in melanocytes, including melanogenesis, is changed in vitiligo patients. We evaluated possible changes in the expression of interleukin (IL)-10 family cytokines (IL26, IL-28A, IL28B, IL29), their receptor subunits (IL20RB, IL22RA2, IL28RA), and genes potentially related to functioning of melanocytes (MDM1, IFNA1, IFNB1, IFNG, and ICAM1) in the case of vitiligo. We observed mRNA expression in vitiligo patients' and controls' skin and peripheral blood mononuclear cells using quantitative real-time polymerase chain reaction. The mRNA expression pattern of IL20RB, IL22RA2, IL-28A, IL28B, IL28RA, MDM1, IFNA1, IFNB1, IFNG, and ICAM1 changed in vitiligo skin and/or peripheral blood mononuclear cells (PBMC) compared with controls. All of these genes may potentially be involved in vitiligo pathogenesis through controlling or participating in different pathways that regulate survival/apoptosis, development and migration of melanocytes, and melanogenesis. This study presents additional support for our previous findings about the importance of IL-10 family cytokines in vitiligo, in particular the possible involvement of IL-22. Further studies should be considered.
authors.affiliation: Department of Physiology, University of Tartu, Tartu, Estonia.
authors.forename: Ene
authors.initials: E
authors.lastname: Reimann
authors.forename: Külli
authors.initials: K
authors.lastname: Kingo
authors.forename: Maire
authors.initials: M
authors.lastname: Karelson
authors.forename: Paula
authors.initials: P
authors.lastname: Reemann
authors.forename: Ulvi
authors.initials: U
authors.lastname: Loite
authors.forename: Helen
authors.initials: H
authors.lastname: Sulakatko
authors.forename: Maris
authors.initials: M
authors.lastname: Keermann
authors.forename: Kristi
authors.initials: K
authors.lastname: Raud
authors.forename: Kristi
authors.initials: K
authors.lastname: Abram
authors.forename: Eero
authors.initials: E
authors.lastname: Vasar
authors.forename: Helgi
authors.initials: H
authors.lastname: Silm
authors.forename: Sulev
authors.initials: S
authors.lastname: Kõks
mesh_terms: D000328:Adult; D000368:Aged; D001706:Biopsy; D016207:Cytokines / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D008544:Melanocytes / Q000378:metabolism*; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism; D012867:Skin / Q000473:pathology; D014820:Vitiligo / Q000235:genetics* / Q000473:pathology*; D055815:Young Adult
chemical_list: D016207:Cytokines; D012333:RNA, Messenger