title: 2,3,5,4'- tetrahydroxystilbene-2-O-β-D-glycoside biosynthesis by suspension cells cultures of Polygonum multiflorum Thunb and production enhancement by methyl jasmonate and salicylic acid.
abstract: Friable calli of Polygonum multiflorum Thunb have been induced in MS medium supplemented with 6-benzylaminopurine (6-BA) and kinetin (KT). Suspension cultures were initiated from friable calli by inoculating calli in liquid MS medium in shake flasks in the dark and 25 °C on an orbital shaker at 100 rpm. The maximum dry weight (DW, 7.85 g/L) and 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glycoside (THSG, 56.39 mg/L) of suspension cells was obtained in MS medium after 16 days culture. Both methyl jasmonate (MeJA) and salicylic acid (SA) could increase THSG production. The most appropriate concentration of MeJA was 100 μmol/L in MS medium, in which concentration THSG content reached the maximum value of 147.79 mg/L, which represented a 162.36% increase compared to that of the control (56.33 mg/L). The most appropriate concentration of SA was 125 μmol/L in MS medium, at which concentration THSG content reached its maximum value of 116.43 mg/L, a 106.69% increase compared to that of the control (56.33 mg/L).
authors.affiliation: School of Bioscience and bioengineering, South China University of Technology, Guangzhou 510006, China. gzzsjzhs@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: Shao
authors.forename: Shu-Jin
authors.initials: SJ
authors.lastname: Zhao
authors.forename: Tang-Bing
authors.initials: TB
authors.lastname: Cui
authors.forename: Zhong-Yu
authors.initials: ZY
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
mesh_terms: D000085:Acetates / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods; D003517:Cyclopentanes / Q000378:metabolism*; D005960:Glucosides / Q000096:biosynthesis*; D054883:Oxylipins / Q000378:metabolism*; D059828:Plant Cells / Q000378:metabolism; D028502:Polygonum / Q000166:cytology / Q000378:metabolism*; D020156:Salicylic Acid / Q000378:metabolism*; D013267:Stilbenes; D013535:Suspensions
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D005960:Glucosides; D054883:Oxylipins; D013267:Stilbenes; D013535:Suspensions; C093026:2,3,5,4'-tetrahydroxystilbene 2-O-glucopyranoside; C072239:methyl jasmonate; D020156:Salicylic Acid