title: Detection of carbapenemase producers in Enterobacteriaceae by use of a novel screening medium.
abstract: A Drigalski agar-based culture medium containing an ertapenem, cloxacillin, and zinc sulfate (Supercarba medium) was tested for screening carbapenemase-producing members of the family Enterobacteriaceae. OXA-48 (n = 44), NDM (n = 25), VIM or IMP (n = 27), and KPC producers (n = 18) were detected with a low detection limit. Its overall sensitivity (95.6%) was higher than those of the currently available ChromID ESBL (bioMérieux) and CHROMagar KPC (CHROMagar) screening media. The Supercarba medium provides a significant improvement for detection of the most common types of carbapenemase producers.
authors.affiliation: Service de Bactériologie-Virologie, INSERM U914 Emerging Resistance to Antibiotics, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris Sud, K-Bicêtre, France. nordmann.patrice@bct.aphp.fr
authors.forename: Patrice
authors.initials: P
authors.lastname: Nordmann
authors.forename: Delphine
authors.initials: D
authors.lastname: Girlich
authors.forename: Laurent
authors.initials: L
authors.lastname: Poirel
mesh_terms: D000362:Agar; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000378:metabolism*; D001431:Bacteriological Techniques / Q000379:methods*; D015169:Colony Count, Microbial; D003470:Culture Media / Q000737:chemistry*; D004755:Enterobacteriaceae / Q000201:enzymology*; D006801:Humans; D008403:Mass Screening / Q000379:methods*; D008826:Microbial Sensitivity Tests; D012680:Sensitivity and Specificity; D001618:beta-Lactamases / Q000378:metabolism*; D047090:beta-Lactams / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D003470:Culture Media; D047090:beta-Lactams; D000362:Agar; D001618:beta-Lactamases; C063912:carbapenemase