title: Neuronal expression of class 6 semaphorins in zebrafish.
abstract: Semaphorins are a large family of guidance molecules identified by an extracellular SEMA domain. Classes 1 and 2 are derived from invertebrates, classes 3-7 are vertebrate and class 8 (v) are viral semaphorins. Class 6 semaphorins are reported to have a wide variety of roles including in axon guidance, transcriptional regulation and cancer. Here we report the identification and expression of four class 6 semaphorins (6A, 6Ba, 6Bb and 6Dl) in three stages of larval development in zebrafish (24, 48 and 72 hours postfertilization). Our data indicate that each of the class 6 semaphorins shows a distinct pattern of expression in the developing nervous system that is dynamic over the first 3 days of embryonic development. These data suggest that the individual class 6 semaphorins have diverse roles in nervous system development.
authors.affiliation: Hotchkiss Brain Institute, Department of Cell Biology and Anatomy, University of Calgary, Calgary, Alberta, Canada.
authors.forename: A M
authors.initials: AM
authors.lastname: Ebert
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, Alberta, Canada.
authors.forename: R E
authors.initials: RE
authors.lastname: Lamont
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, Alberta, Canada.
authors.forename: S J
authors.initials: SJ
authors.lastname: Childs
authors.affiliation: Hotchkiss Brain Institute, Department of Cell Biology and Anatomy, University of Calgary, Calgary, Alberta, Canada.
authors.forename: S
authors.initials: S
authors.lastname: McFarlane
mesh_terms: D000818:Animals; D001369:Axons / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D008636:Mesencephalon / Q000166:cytology / Q000378:metabolism; D010802:Phylogeny; D012165:Retinal Ganglion Cells / Q000378:metabolism; D039961:Semaphorins / Q000235:genetics* / Q000378:metabolism; D013477:Superior Colliculi / Q000166:cytology / Q000378:metabolism; D015027:Zebrafish / Q000196:embryology / Q000235:genetics*; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: C000590393:Sema6a protein, zebrafish; D039961:Semaphorins; D029961:Zebrafish Proteins; C526275:semaphorin 6D, zebrafish