title: Comparative HPLC-MS analysis of canine and human meibomian lipidomes: many similarities, a few differences.
abstract: The aim of this study was to evaluate the lipidome of meibomian gland secretions in canines (cMGS) - a common pet and laboratory animal - and to compare it with that of human MGS (hMGS), to determine whether canines could be used as a valid experimental animal model in studies of the biochemistry and physiology of the human ocular surface in general, and of the Meibomian glands in particular. The MGS of both species were evaluated using HPLC in combination with atmospheric pressure chemical ionization ion trap mass spectrometry. The main lipid classes found in cMGS were very long chain cholesteryl esters, wax esters, (O-acyl)-omega-hydroxy fatty acids (OAHFA), and cholesteryl esters of OAHFA. The lipidomes of cMGS and hMGS were found to be qualitatively similar, which implies similar biosynthetic and biodegradation pathways in canines and humans. However, some quantitative differences between the two were observed.
authors.affiliation: University of Texas Southwestern Medical Center, Department of Ophthalmology and the Graduate School of Biomedical Sciences, Dallas, TX, USA. igor.butovich@utsouthwestern.edu
authors.forename: Igor A
authors.initials: IA
authors.lastname: Butovich
authors.forename: Anna M
authors.initials: AM
authors.lastname: Borowiak
authors.forename: J Corinna
authors.initials: JC
authors.lastname: Eule
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004285:Dogs; D006801:Humans; D050356:Lipid Metabolism; D013058:Mass Spectrometry; D008537:Meibomian Glands / Q000378:metabolism*; D008055:Lipids
chemical_list: D008055:Lipids