title: Evaluation of the matrix effect of thermophilic anaerobic digestion on inactivation of infectious laryngotracheitis virus using real-time PCR and viral cell culture.
abstract: The matrix effect of the thermophilic anaerobic digestion (TAD) process on inactivation of infectious laryngotracheitis virus (ILTV) was evaluated. Viral cell culture and real-time PCR were used for assessing removal of the viral infectivity and degradation of viral DNA, respectively. Results showed that the TAD-derived matrix alone can inactivate the virus and destroy the nucleic acid helix core of ILTV in a time-and- dose-dependent manner. No cytopathogenic effect (CPE) was observed in the cells exposed to ILTV pre-treated with TAD matrix for 1.5h in experiment 1 and for 16h in experiment 2. There was a significant statistical difference between TAD matrix treated and non-treated cultures (p<0.001, Chi-test). Amplifiable ILT viral DNA was reduced 2.27 log by 1.5h-treatment and was not present by 16h-treatment with TAD matrix, indicating complete viral DNA fragmentation. The TAD process is an environmentally friendly way for disposing of poultry biowaste and carcasses.
authors.affiliation: Highmark Renewables Research LP, AFDP Room-1-037, 6004-118 Street, Edmonton, Alberta, Canada T6G 2E1. tj@highmark.ca
authors.forename: Tiejun
authors.initials: T
authors.lastname: Gao
authors.forename: Evelyn
authors.initials: E
authors.lastname: Bowlby
authors.forename: Yupin
authors.initials: Y
authors.lastname: Tong
authors.forename: John T Y
authors.initials: JT
authors.lastname: Wu
authors.forename: Lester
authors.initials: L
authors.lastname: Wong
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Tower
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Pang
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Li
mesh_terms: D000693:Anaerobiosis; D045744:Cell Line, Tumor; D003588:Cytopathogenic Effect, Viral; D001352:Herpesvirus 1, Gallid / Q000187:drug effects* / Q000235:genetics / Q000472:pathogenicity; D006801:Humans; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*