title: CpG methylation as a tool to characterize cell-free Kaposi sarcoma herpesvirus DNA.
abstract: We studied the presence of Kaposi sarcoma herpesvirus sequences in cell-free DNA (cfDNA) isolated from the blood of patients with AIDS-related Kaposi sarcoma (KS) and primary effusion lymphoma (PEL). The use of paramagnetic beads linked to methyl-CpG binding domain protein allowed separation of virion and cell-derived DNA. Only virion DNA was detected in the blood of KS patients, whereas cell-derived DNA was detected in a patient with AIDS-related PEL. The difference in the origins of cfDNA in these settings may in part reflect very different proliferative indices in KS and PEL tumor tissue.
authors.affiliation: Department of Oncology, Johns Hopkins School of Medicine, Baltimore, Maryland 21287, USA.
authors.forename: Meir
authors.initials: M
authors.lastname: Shamay
authors.forename: Nicholas
authors.initials: N
authors.lastname: Hand
authors.forename: M Victor
authors.initials: MV
authors.lastname: Lemas
authors.forename: Henry B
authors.initials: HB
authors.lastname: Koon
authors.forename: Susan E
authors.initials: SE
authors.lastname: Krown
authors.forename: John
authors.initials: J
authors.lastname: Wrangle
authors.forename: Prashant
authors.initials: P
authors.lastname: Desai
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Ramos
authors.forename: Richard F
authors.initials: RF
authors.lastname: Ambinder
mesh_terms: D004279:DNA, Viral / Q000737:chemistry* / Q000302:isolation & purification; D015257:DNA-Cytosine Methylases / Q000378:metabolism*; D019288:Herpesvirus 8, Human / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D054685:Lymphoma, Primary Effusion / Q000821:virology*; D008745:Methylation; D012514:Sarcoma, Kaposi / Q000821:virology*
chemical_list: D004279:DNA, Viral; C081573:DNA modification methylase SssI; D015257:DNA-Cytosine Methylases