title: Fructanase and fructosyltransferase activity of non-Saccharomyces yeasts isolated from fermenting musts of Mezcal.
abstract: Fructanase and fructosyltransferase are interesting for the tequila process and prebiotics production (functional food industry). In this study, one hundred thirty non-Saccharomyces yeasts isolated from "Mezcal de Oaxaca" were screened for fructanase and fructosyltransferase activity. On solid medium, fifty isolates grew on Agave tequilana fructans (ATF), inulin or levan. In liquid media, inulin and ATF induced fructanase activities of between 0.02 and 0.27U/ml depending of yeast isolate. High fructanase activity on sucrose was observed for Kluyveromyces marxianus and Torulaspora delbrueckii, while the highest fructanase activity on inulin and ATF was observed for Issatchenkia orientalis, Cryptococcus albidus, and Candida apicola. Zygosaccharomyces bisporus and Candida boidinii had a high hydrolytic activity on levan. Sixteen yeasts belonging to K. marxianus, T. delbrueckii and C. apicola species were positive for fructosyltransferase activity. Mezcal microbiota proved to showed to be a source for new fructanase and fructosyltransferases with potential application in the tequila and food industry.
authors.affiliation: Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C., Avenida Normalistas # 800, Col. Colinas de la Normal, 44270 Guadalajara, Jalisco, Mexico.
authors.forename: Javier
authors.initials: J
authors.lastname: Arrizon
authors.forename: Sandrine
authors.initials: S
authors.lastname: Morel
authors.forename: Anne
authors.initials: A
authors.lastname: Gschaedler
authors.forename: Pierre
authors.initials: P
authors.lastname: Monsan
mesh_terms: D027882:Agave / Q000201:enzymology*; D003470:Culture Media; D005285:Fermentation; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006602:Hexosyltransferases / Q000378:metabolism*; D015003:Yeasts / Q000201:enzymology*; D001426:Bacterial Proteins
chemical_list: D003470:Culture Media; D006026:Glycoside Hydrolases; D006602:Hexosyltransferases; C021451:inulosucrase; D001426:Bacterial Proteins; C021512:levanase