title: Following glucose oxidase activity by chemiluminescence and chemiluminescence resonance energy transfer (CRET) processes involving enzyme-DNAzyme conjugates.
abstract: A hybrid consisting of glucose oxidase-functionalized with hemin/G-quadruplex units is used for the chemiluminescence detection of glucose. The glucose oxidase-mediated oxidation of glucose yields gluconic acid and H(2)O(2). The latter in the presence of luminol acts as substrate for the hemin/G-quadruplex-catalyzed generation of chemiluminescence. The glucose oxidase/hemin G-quadruplex hybrid was immobilized on CdSe/ZnS quantum dots (QDs). The light generated by the hybrid, in the presence of glucose, activated a chemiluminescence resonance energy transfer process to the QDs, resulting in the luminescence of the QDs. The intensities of the luminescence of the QDs at different concentrations of glucose provided an optical means to detect glucose.
authors.affiliation: Institute of Chemistry, Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
authors.forename: Angelica
authors.initials: A
authors.lastname: Niazov
authors.forename: Ronit
authors.initials: R
authors.lastname: Freeman
authors.forename: Julia
authors.initials: J
authors.lastname: Girsh
authors.forename: Itamar
authors.initials: I
authors.lastname: Willner
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology; D060807:Bioluminescence Resonance Energy Transfer Techniques / Q000379:methods*; D015374:Biosensing Techniques / Q000379:methods*; D019187:Cadmium Compounds / Q000737:chemistry; D002138:Calibration; D003432:Cross-Linking Reagents / Q000737:chemistry; D021881:DNA, Catalytic / Q000737:chemistry / Q000378:metabolism*; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism*; D020723:Glucuronic Acid / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000032:analysis / Q000378:metabolism; D008163:Luminescent Measurements / Q000379:methods*; D008165:Luminol / Q000737:chemistry; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism; D045663:Quantum Dots; D018036:Selenium Compounds / Q000737:chemistry; D013440:Sulfides / Q000737:chemistry; D017967:Zinc Compounds / Q000737:chemistry
chemical_list: D019187:Cadmium Compounds; D003432:Cross-Linking Reagents; D021881:DNA, Catalytic; D009838:Oligodeoxyribonucleotides; D018036:Selenium Compounds; D013440:Sulfides; D017967:Zinc Compounds; D008165:Luminol; D020723:Glucuronic Acid; C058667:cadmium selenide; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D005947:Glucose; C031238:zinc sulfide