title: Human primary cementoblasts respond to combined IL-1β stimulation and compression with an impaired BSP and CEMP-1 expression.
abstract: Cementoblasts are cells that produce, secrete and direct the production of cementum. Resorption lacunae occur in over 90% of teeth at the compression side of the periodontal ligament and might result in an irreversible loss of the original root length. We isolated and cultivated human primary cementoblasts and investigated their expression pattern concerning markers of the osteoblastogenic differentiation (RUNX2, OCN, ALP, and BSP) and CEMP-1. Compared to osteoblasts, cementoblasts displayed an expression pattern comparable to osteoblasts in an early stage of osteoblastogenic differentiation. Next, the human primary cementoblasts were stimulated with IL-1β (1 and 10ng/ml) for 24 and 96h and subsequently subjected to compressive forces (30.3g/cm(2)) for 1 and 6h. Our in vitro data demonstrated that BSP and CEMP-1 expression significantly decreased when stimulation was accompanied by compression, while compression or stimulation alone led to increased levels of BSP and decreased levels of CEMP-1. We concluded that human primary cementoblasts subjected to compression and IL-1β stimulation impeded BSP and CEMP-1 expression, proteins that are associated with cementogenesis.
authors.affiliation: Department of Orthodontics and Dentofacial Orthopedics, Dental School, University of Heidelberg, Im Neuenheimer Feld 400, 69120 Heidelberg, Germany. katja.diercke@med.uni-heidelberg.de
authors.forename: K
authors.initials: K
authors.lastname: Diercke
authors.forename: A
authors.initials: A
authors.lastname: König
authors.forename: A
authors.initials: A
authors.lastname: Kohl
authors.forename: C J
authors.initials: CJ
authors.lastname: Lux
authors.forename: R
authors.initials: R
authors.lastname: Erber
mesh_terms: D000293:Adolescent; D000328:Adult; D000943:Antigens, Differentiation / Q000096:biosynthesis; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D002648:Child; D019245:Compressive Strength; D003739:Dental Cementum / Q000166:cytology / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D058574:Integrin-Binding Sialoprotein / Q000096:biosynthesis*; D053583:Interleukin-1beta / Q000378:metabolism* / Q000494:pharmacology; D008297:Male; D011506:Proteins / Q000378:metabolism*; D013312:Stress, Physiological / Q000187:drug effects / Q000502:physiology*; D013997:Time Factors
chemical_list: D000943:Antigens, Differentiation; C510886:CEMP1 protein, human; C508585:IL1B protein, human; D058574:Integrin-Binding Sialoprotein; D053583:Interleukin-1beta; D011506:Proteins