title: Complete mitogenome sequence of black carp (Mylopharyngodon piceus) and its use for molecular phylogeny of leuciscine fishes.
abstract: The black carp Mylopharyngodon piceus (Cyprinidae), native to eastern Asian, is a large, commercially important fish, and has been introduced to many other countries for variable reasons. In this study, the complete mitochondrial genome sequences from three specimens of black carp were first determined and were used to evaluate the sister relationship between black carp and grass carp (Ctenopharyngodon idellus). Two individuals had a mitogenome of 16,609 bp, while the other was 16,611 bp in length. Similar to most vertebrates, the black carp contains the same gene order and an identical number of genes or regions, including 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and 1 putative control region. Phylogenetic analyses using three different computational algorithms (Maximum Parsimony, Maximum Likelihood, and Bayesian analysis) revealed two distinct clades in subfamily Leuciscinae. However, the sister taxonomic relationship of black carp and grass carp was not observed using sequences of nearly complete mitochondrial genomes, which suggests more nuclear gene markers are needed to resolve the phylogenetic relationship between black carp and grass carp.
authors.affiliation: Key Laboratory of Aquatic Genetic Resources and Utilization Certificated by Ministry of Agriculture, Shanghai Ocean University, Shanghai, 201306, China. wangch@shou.edu.cn
authors.forename: Chenghui
authors.initials: C
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Jinquan
authors.initials: J
authors.lastname: Yang
authors.forename: Guoqing
authors.initials: G
authors.lastname: Lu
authors.forename: Xiao
authors.initials: X
authors.lastname: Song
authors.forename: Qin
authors.initials: Q
authors.lastname: Chen
authors.forename: Jiawei
authors.initials: J
authors.lastname: Xu
authors.forename: Qinling
authors.initials: Q
authors.lastname: Yang
authors.forename: Sifa
authors.initials: S
authors.lastname: Li
mesh_terms: D000818:Animals; D001483:Base Sequence; D001499:Bayes Theorem; D002347:Carps / Q000235:genetics*; D021901:DNA, Intergenic / Q000235:genetics; D005399:Fishes / Q000145:classification* / Q000235:genetics*; D050259:Genes, Mitochondrial / Q000235:genetics; D054629:Genome, Mitochondrial / Q000235:genetics*; D016013:Likelihood Functions; D009711:Nucleotides / Q000235:genetics; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D011110:Polymorphism, Genetic; D012343:RNA, Transfer / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D021901:DNA, Intergenic; D009711:Nucleotides; D012343:RNA, Transfer