title: Isolation and identification of Mycobacterium avium subspecies silvaticum from a horse.
abstract: Routine cultivation methods are able to distinguish between isolates of the Mycobacterium avium and the Mycobacterium tuberculosis complex. However, molecular tools are needed to further identify the several subspecies in the M. avium complex, especially for the subspecies avium and silvaticum. A rapid technique using HhaI restriction digestion of a 349 bp amplification product of the 85B antigen (α-antigen) gene was used for the identification of M. avium subsp. silvaticum in a three-year-old gelding presenting with caseous, necrotizing, granulomatous lesions. The result was confirmed by sequencing of the 85B antigen gene.
authors.affiliation: Department of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium. koen.chiers@ugent.be
authors.forename: Koen
authors.initials: K
authors.lastname: Chiers
authors.forename: Pieter
authors.initials: P
authors.lastname: Deschaght
authors.forename: Thierry
authors.initials: T
authors.lastname: De Baere
authors.forename: Slawomir
authors.initials: S
authors.lastname: Dabrowski
authors.forename: Roman
authors.initials: R
authors.lastname: Kotlowski
authors.forename: Dominique
authors.initials: D
authors.lastname: De Clercq
authors.forename: Richard
authors.initials: R
authors.lastname: Ducatelle
authors.forename: Mario
authors.initials: M
authors.lastname: Vaneechoutte
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology; D015373:Bacterial Typing Techniques; D004262:DNA Restriction Enzymes / Q000235:genetics; D006734:Horse Diseases / Q000175:diagnosis / Q000276:immunology / Q000382:microbiology*; D006736:Horses; D008297:Male; D009162:Mycobacterium avium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D014376:Tuberculosis / Q000175:diagnosis / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*
chemical_list: D000942:Antigens, Bacterial; D004262:DNA Restriction Enzymes