title: [Alternative scaffold proteins].
abstract: Review is devoted to the challenging direction in modem molecular biology and bioengineering - the properties of alternative scaffold proteins (ASP) and methods for obtaining ASP binding molecules. ASP molecules incorporate conservative protein core and hypervariable regions, providing for the binding function. Structural classification of ASP includes several types which differ also in their molecular targets and potential applications. Construction of artificial binding proteins on the ASP basis implies a combinatorial library design with subsequent selection of specific binders with the use of phage display or the modem cell-free systems. Alternative binding proteins on non-immunoglobulin scaffolds find broad applications in different fields ofbiotechnology and molecular medicine.
authors.forename: L E
authors.initials: LE
authors.lastname: Petrovskaia
authors.forename: L N
authors.initials: LN
authors.lastname: Shingarova
authors.forename: D A
authors.initials: DA
authors.lastname: Dolgikh
authors.forename: M P
authors.initials: MP
authors.lastname: Kirpichnikov
mesh_terms: D000906:Antibodies / Q000737:chemistry*; D000915:Antibody Affinity; D000918:Antibody Specificity; D002352:Carrier Proteins / Q000737:chemistry; D006801:Humans; D008024:Ligands; D034521:Nuclear Matrix-Associated Proteins / Q000737:chemistry* / Q000235:genetics; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics; D019151:Peptide Library; D015202:Protein Engineering / Q000379:methods*; D017434:Protein Structure, Tertiary
chemical_list: D000906:Antibodies; D002352:Carrier Proteins; D008024:Ligands; D034521:Nuclear Matrix-Associated Proteins; D010446:Peptide Fragments; D019151:Peptide Library