title: IFT proteins accumulate during cell division and localize to the cleavage furrow in Chlamydomonas.
abstract: Intraflagellar transport (IFT) proteins are well established as conserved mediators of flagellum/cilium assembly and disassembly. However, data has begun to accumulate in support of IFT protein involvement in other processes elsewhere in the cell. Here, we used synchronous cultures of Chlamydomonas to investigate the temporal patterns of accumulation and localization of IFT proteins during the cell cycle. Their mRNAs showed periodic expression that peaked during S and M phase (S/M). Unlike most proteins that are synthesized continuously during G1 phase, IFT27 and IFT46 levels were found to increase only during S/M phase. During cell division, IFT27, IFT46, IFT72, and IFT139 re-localized from the flagella and basal bodies to the cleavage furrow. IFT27 was further shown to be associated with membrane vesicles in this region. This localization pattern suggests a role for IFT in cell division.
authors.affiliation: Department of Molecular Cellular and Developmental Biology, Yale University, New Haven, Connecticut, United States of America.
authors.forename: Christopher R
authors.initials: CR
authors.lastname: Wood
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Wang
authors.forename: Dennis
authors.initials: D
authors.lastname: Diener
authors.forename: James Matt
authors.initials: JM
authors.lastname: Zones
authors.forename: Joel
authors.initials: J
authors.lastname: Rosenbaum
authors.forename: James G
authors.initials: JG
authors.lastname: Umen
mesh_terms: D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002453:Cell Cycle / Q000235:genetics / Q000502:physiology; D002455:Cell Division / Q000235:genetics / Q000502:physiology; D002696:Chlamydomonas / Q000235:genetics / Q000378:metabolism*; D005407:Flagella / Q000378:metabolism; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D015800:Protozoan Proteins