title: Stability of toxigenicity in proteolytic Clostridium botulinum type B upon serial passage.
abstract: Proteolytic Clostridium botulinum type B strains were investigated for stability of toxigenicity and bont/b gene upon serial passage. Strains with bont/b gene located on their plasmids showed loss or decrease of toxigenicity during serial passage. Some strains lost the bont/b gene-encoding plasmid. The stability of the plasmids varied between strains.
authors.affiliation: Department of Microbiology, Osaka City Institute of Public Health and Environmental Sciences, 8-34 Tojo-cho, Tennoji-ku, Osaka 543-0026, Japan.
authors.forename: Kaoru
authors.initials: K
authors.lastname: Umeda
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Seto
authors.forename: Tomoko
authors.initials: T
authors.lastname: Kohda
authors.forename: Masafumi
authors.initials: M
authors.lastname: Mukamoto
authors.forename: Shunji
authors.initials: S
authors.lastname: Kozaki
mesh_terms: D001905:Botulinum Toxins / Q000235:genetics* / Q000378:metabolism / Q000633:toxicity; D001906:Botulism / Q000382:microbiology*; D047014:Clostridium botulinum type B / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity*; D006801:Humans; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D012692:Serial Passage; D014774:Virulence
chemical_list: D001905:Botulinum Toxins