title: CpG-enriched plasmid enhances the efficacy of the traditional foot-and-mouth disease killed vaccine.
abstract: A CpG-enriched recombinant plasmid (pUC18-CpG) as an adjuvant of FMD killed vaccine was tested for immunization and vaccination challenge in a porcine model. Our preliminary results had indicated that the recombinant plasmid could enhance the humoral immune response triggered by the traditional oil-adjuvant vaccine after the initial inoculation. A subsequent vaccination-challenge test showed an increased PD(50)  value. Thus, coadministration of the recombinant plasmid with the oil-adjuvant vaccine helped illicit an immune response earlier than that elicited by giving the vaccine alone. Our results showed that pUC18-CpG can be a potent immunoadjuvant for the traditional FMD killed vaccine and can greatly enhance the traditional vaccine's efficacy when given in combination with it.
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Guo
authors.forename: Hong
authors.initials: H
authors.lastname: Jia
authors.forename: Quan
authors.initials: Q
authors.lastname: Zhang
authors.forename: Weifeng
authors.initials: W
authors.lastname: Yuan
authors.forename: Gang
authors.initials: G
authors.lastname: Zhu
authors.forename: Ting
authors.initials: T
authors.lastname: Xin
authors.forename: Hongfei
authors.initials: H
authors.lastname: Zhu
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Sánchez-Vizcaíno
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000235:genetics; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D005536:Foot-and-Mouth Disease / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics / Q000276:immunology*; D010957:Plasmids / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D013552:Swine; D013553:Swine Diseases / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D014611:Vaccination; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D015164:Vaccines, Inactivated; D014765:Viral Vaccines