title: Lytic activity of LysH5 endolysin secreted by Lactococcus lactis using the secretion signal sequence of bacteriocin Lcn972.
abstract: Bacteriophage endolysins have an interesting potential as antimicrobials. The endolysin LysH5, encoded by Staphylococcus aureus phage vB_SauS-phi-IPLA88, was expressed and secreted in Lactococcus lactis using the signal peptide of bacteriocin lactococcin 972 and lactococcal constitutive and inducible promoters. Up to 80 U/mg of extracellular active endolysin was detected in culture supernatants, but most of the protein (up to 323 U/mg) remained in the cell extracts.
authors.affiliation: Instituto de Productos Lácteos de Asturias (IPLA-CSIC), Villaviciosa, Asturias, Spain.
authors.forename: Lorena
authors.initials: L
authors.lastname: Rodríguez-Rubio
authors.forename: Dolores
authors.initials: D
authors.lastname: Gutiérrez
authors.forename: Beatriz
authors.initials: B
authors.lastname: Martínez
authors.forename: Ana
authors.initials: A
authors.lastname: Rodríguez
authors.forename: Pilar
authors.initials: P
authors.lastname: García
mesh_terms: D001430:Bacteriocins / Q000235:genetics*; D001433:Bacteriolysis; D001435:Bacteriophages / Q000201:enzymology / Q000235:genetics; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D013294:Lactococcus lactis / Q000201:enzymology* / Q000235:genetics; D011401:Promoter Regions, Genetic; D021382:Protein Sorting Signals; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013211:Staphylococcus aureus / Q000201:enzymology / Q000235:genetics
chemical_list: D001430:Bacteriocins; D021382:Protein Sorting Signals; D011994:Recombinant Proteins; C402706:lactococcin 972; D010450:Endopeptidases; C001143:endolysin