title: Native chemical ligation at glutamine.
abstract: The desulfurization reaction introduced by Yan and Dawson as a postnative chemical ligation step greatly expanded the scope of ligation chemistry beyond Xaa-Cys (Xaa is any amino acid) by making ligation at Xaa-Phe, Xaa-Val, Xaa-Lys, Xaa-Leu, Xaa-Thr, and Xaa-Pro junctions accessible in the synthesis of functional proteins. A new ligation site based on Xaa-Gln utilizing γ-mercaptoglutamine is reported, and several examples on the efficiency of ligation coupled with desulfurization are provided.
authors.affiliation: Department of Chemistry, Ben-Gurion University of the Negev, Beer Sheva, 84105, Israel.
authors.forename: Peter
authors.initials: P
authors.lastname: Siman
authors.forename: Subramanian Vedhanarayanan
authors.initials: SV
authors.lastname: Karthikeyan
authors.forename: Ashraf
authors.initials: A
authors.lastname: Brik
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D005973:Glutamine / Q000737:chemistry*; D008956:Models, Chemical; D015394:Molecular Structure; D011506:Proteins / Q000138:chemical synthesis* / Q000737:chemistry; D013455:Sulfur / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D011506:Proteins; D005973:Glutamine; D013455:Sulfur