title: The role of nocturnin in early adipogenesis and modulation of systemic insulin resistance in human.
abstract: The deadenylase nocturnin (Noc, Ccrn4l) has been recently found to regulate lipid metabolism and to control preadipocyte differentiation. Here, we showed that among the five deadenylases tested, Noc and Pan2 exhibited a biphasic expression which is out of phase to each other during adipocyte differentiation of 3T3-L1 cells. The expression levels of other deadenylases, including Parn, Ccr4, and Caf1, were relatively unchanged or reduced. The immediate early expressed Noc during 3T3-L1 adipogenesis was involved in regulating mitotic clonal expansion (MCE) and cyclin D1 expression, as demonstrated in Noc-silenced 3T3-L1 cells and Noc(-/-) primary mouse embryonic fibroblasts (MEFs). Transcriptional profiling of Noc-depleted 3T3-L1 adipocytes revealed that most of the differentially expressed genes were related to cell growth and proliferation. In human adipose tissue, NOC mRNA level negatively associated with both fasting serum insulin and homeostasis model assessment of insulin resistance, and positively associated with both adiponectin mRNA levels and circulating adiponectin levels. Taken together, these results suggest the role of Noc in the modulation of early adipogenesis as well as systemic insulin sensitivity.
authors.affiliation: Institute of Molecular Medicine, National Taiwan University, Taipei, Taiwan.
authors.forename: Siow-Wey
authors.initials: SW
authors.lastname: Hee
authors.forename: Shu-Huei
authors.initials: SH
authors.lastname: Tsai
authors.forename: Yi-Cheng
authors.initials: YC
authors.lastname: Chang
authors.forename: Ching-Jin
authors.initials: CJ
authors.lastname: Chang
authors.forename: I-Shing
authors.initials: IS
authors.lastname: Yu
authors.forename: Po-Chu
authors.initials: PC
authors.lastname: Lee
authors.forename: Wei-Jei
authors.initials: WJ
authors.lastname: Lee
authors.forename: Emily
authors.initials: E
authors.lastname: Yun-Chia Chang
authors.forename: Lee-Ming
authors.initials: LM
authors.lastname: Chuang
mesh_terms: D041721:3T3-L1 Cells; D050156:Adipogenesis / Q000235:genetics / Q000502:physiology*; D052242:Adiponectin / Q000097:blood / Q000235:genetics / Q000378:metabolism; D000273:Adipose Tissue / Q000378:metabolism* / Q000502:physiology; D000328:Adult; D000818:Animals; D049109:Cell Proliferation; D019938:Cyclin D1 / Q000378:metabolism; D005215:Fasting; D005347:Fibroblasts / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008938:Mitosis; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D009765:Obesity / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D059467:Transcriptome
chemical_list: D052242:Adiponectin; D007328:Insulin; D009687:Nuclear Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; C102804:nocturnin; D019938:Cyclin D1