title: Detecting DNA methylation using the methylated CpG island amplification and microarray technique.
abstract: Methylated CpG island amplification and microarray (MCAM) is a two-color array technique that quantifies DNA methylation by hybridizing equimolar amounts of treated vs. control cell-line DNA to an array platform. Sample preparation, hybridization, and scanning are performed over 1 week, but multiple samples can be prepared simultaneously, allowing for high-throughput processing and data acquisition. One microarray slide is required for each sample assayed, with a control sample hybridized with each test sample.
authors.affiliation: Haematology, Centre for Cancer Research and Cell Biology Belfast, Queen's University Belfast, Belfast, Northern Ireland, UK. hcolyer01@qub.ac.uk
authors.forename: Hilary A A
authors.initials: HA
authors.lastname: Colyer
authors.forename: Margaret
authors.initials: M
authors.lastname: Dellett
authors.forename: Ken I
authors.initials: KI
authors.lastname: Mills
mesh_terms: D018899:CpG Islands / Q000235:genetics*; D019175:DNA Methylation / Q000235:genetics*; D057890:Epigenomics / Q000379:methods*; D046228:Microarray Analysis / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods