title: Phenotypical and functional characteristics of mesenchymal stem cells from bone marrow: comparison of culture using different media supplemented with human platelet lysate or fetal bovine serum.
abstract: BACKGROUND
  Mesenchymal stem cells (MSCs) are multipotent cells able to differentiate into several mesenchymal lineages, classically derived from bone marrow (BM) but potentially from umbilical cord blood (UCB). Although they are becoming a good tool for regenerative medicine, they usually need to be expanded in fetal bovine serum (FBS)-supplemented media. Human platelet lysate (HPL) has recently been proposed as substitute for safety reasons, but it is not yet clear how this supplement influences the properties of expanded MSCs.
  
  
  METHODS
  In the present study, we compared the effect of various media combining autologous HPL with or without FBS on phenotypic, proliferative and functional (differentiation, cytokine secretion profile) characteristics of human BM-derived MSCs.
  
  
  RESULTS
  Despite less expression of adipogenic and osteogenic markers, MSCs cultured in HPL-supplemented media fully differentiated along osteoblastic, adipogenic, chondrogenic and vascular smooth muscle lineages. The analyses of particular specific proteins expressed during osteogenic differentiation (calcium-sensing receptor (CaSR) and parathormone receptor (PTHR)) showed their decrease at D0 before any induction for MSC cultured with HPL mostly at high percentage (10%HPL). The cytokine dosage showed a clear increase of proliferation capacity and interleukin (IL)-6 and IL-8 secretion.
  
  
  CONCLUSIONS
  This study shows that MSCs can be expanded in media supplemented with HPL that can totally replace FBS. HPL-supplemented media not only preserves their phenotype as well as their differentiation capacity, but also shortens culture time by increasing their growth rate.
authors.affiliation: Laboratory of Hematopoiesis, UPRES-EA3855, Faculty of Medicine, University François Rabelais, 10 boulevard Tonnellé, 37032 Tours Cedex, France.
authors.forename: Nesrine
authors.initials: N
authors.lastname: Ben Azouna
authors.forename: Faouzi
authors.initials: F
authors.lastname: Jenhani
authors.forename: Zohra
authors.initials: Z
authors.lastname: Regaya
authors.forename: Lamia
authors.initials: L
authors.lastname: Berraeis
authors.forename: Tarek
authors.initials: T
authors.lastname: Ben Othman
authors.forename: Elfi
authors.initials: E
authors.lastname: Ducrocq
authors.forename: Jorge
authors.initials: J
authors.lastname: Domenech
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000166:cytology*; D001854:Bone Marrow Cells / Q000166:cytology; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects; D002457:Cell Extracts / Q000494:pharmacology*; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010641:Phenotype; D044169:Receptors, Calcium-Sensing / Q000378:metabolism; D018016:Receptors, Parathyroid Hormone / Q000378:metabolism
chemical_list: D002457:Cell Extracts; D003470:Culture Media; D015850:Interleukin-6; D016209:Interleukin-8; D044169:Receptors, Calcium-Sensing; D018016:Receptors, Parathyroid Hormone