title: Production of prostaglandin e(2) and i(2) is coupled with cyclooxygenase-2 in human follicular dendritic cells.
abstract: BACKGROUND
  Prostaglandins (PGs) play pathogenic and protective roles in inflammatory diseases. The novel concept of PGs as immune modulators is being documented by several investigators. By establishing an in vitro experimental model containing human follicular dendritic cell-like cells, HK cells, we reported that HK cells produce prostaglandin E(2) (PGE(2)) and prostaglandin I(2) (PGI(2)) and that these PGs regulate biological functions of T and B cells.
  
  
  METHODS
  To investigate the respective contribution of cyclooxygenase-1 (COX-1) and COX-2 to PGE(2) and PGI(2) production in HK cells, we performed siRNA technology to knock down COX enzymes and examined the effect on PG production.
  
  
  RESULTS
  Both PGE(2) and PGI(2) productions were almost completely inhibited by the depletion of COX-2. In contrast, COX-1 knockdown did not significantly affect PG production induced by lipopolysaccharide (LPS).
  
  
  CONCLUSIONS
  The current results suggest that mPGES-1 and PGIS are coupled with COX-2 but not with COX-1 in human follicular dendritic cell (FDC) and may help understand the potential effects of selective COX inhibitors on the humoral immunity.
authors.affiliation: Department of Microbiology and Immunology, School of Medicine, Kangwon National University, Chuncheon 200-701, Korea.
authors.forename: Whajung
authors.initials: W
authors.lastname: Cho
authors.forename: Jini
authors.initials: J
authors.lastname: Kim
authors.forename: Kyu-Bong
authors.initials: KB
authors.lastname: Cho
authors.forename: Jongseon
authors.initials: J
authors.lastname: Choe