title: [Biocatalytic synthesis of pharmacology perspective stigmast-4-en-3-one using Rhodococci cells].
abstract: The conditions for a directed biocatalytic oxidation of beta-sitosterol to a pharmacologically promising stigmast-4-en-3-one using Rhodococcus actinobacteria were selected. It was shown that palmitic acid induced the cholesterol oxidase reaction and allowed for the decrease in the bioconversion process duration from 7 to 5 days. The maximum level ofstigmast-4-ene-3-one formation was achieved using n-hexadecane as an additional growth substrate. With increased concentrations of beta-sitosterol (up to 2 g/L) an effective target product formation (80%) was achieved in the presence of Tween-80 and beta-cyclodextrine. R. erythropolis strains were 1.5-2 times more active than R. ruber strains in catalyzing the beta-sitosterol biotransformation process.
authors.forename: E M
authors.initials: EM
authors.lastname: Nogovitsina
authors.forename: V V
authors.initials: VV
authors.lastname: Grishko
authors.forename: I B
authors.initials: IB
authors.lastname: Ivshina
mesh_terms: D000473:Alkanes / Q000737:chemistry; D055162:Biocatalysis; D001711:Biotransformation; D002789:Cholesterol Oxidase / Q000737:chemistry / Q000378:metabolism; D012240:Rhodococcus / Q000378:metabolism*; D012855:Sitosterols / Q000737:chemistry / Q000378:metabolism*; D013265:Stigmasterol / Q000031:analogs & derivatives* / Q000138:chemical synthesis
chemical_list: D000473:Alkanes; D012855:Sitosterols; C488470:stigmast-4-en-3-one; C025473:gamma-sitosterol; D013265:Stigmasterol; D002789:Cholesterol Oxidase; C007932:n-hexadecane