title: Ginsenoside Rg1 protects human fibroblasts against psoralen- and UVA-induced premature senescence through a telomeric mechanism.
abstract: This study was aimed to investigate the protective effects of ginsenoside Rg1 on 8-methoxypsoralen(8-MOP)/Ultraviolet A (UVA)-induced premature senescence in human fibroblasts, and the underlying mechanism. We established a stress-induced premature senescence model by 8-MOP/UVA irradiation. The aging condition was determined by histochemical staining of senescence-associated β-galactosidase (SA-β-gal). Relative telomere length was calculated by the ratio of the amount of telomere DNA versus single copy DNA by real-time polymerase chain reaction, and protein levels of p-P53, p21(WAF-1) and p16(INK-4a) were estimated by Western blotting. Compared with the 8-MOP/UVA treatment group, we found that the irradiated fibroblasts pretreated with ginsenoside Rg1 demonstrated a decrease in the expression of SA-β-gal, a downregulation in the level of senescence-associated proteins, and a deceleration in telomere shortening. Taken together, these results suggest that ginsenoside Rg1 significantly antagonizes premature senescence induced by 8-MOP/UVA in fibroblasts.
authors.affiliation: Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Bing-Rong
authors.initials: BR
authors.lastname: Zhou
authors.forename: Yang
authors.initials: Y
authors.lastname: Xu
authors.forename: Di
authors.initials: D
authors.lastname: Wu
authors.forename: Felicia
authors.initials: F
authors.lastname: Permatasari
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Gao
authors.forename: Dan
authors.initials: D
authors.lastname: Luo
mesh_terms: D019588:Aging, Premature / Q000188:drug therapy*; D002460:Cell Line; D016922:Cellular Senescence / Q000187:drug effects*; D019610:Cytoprotection; D005347:Fibroblasts / Q000187:drug effects*; D036145:Ginsenosides / Q000494:pharmacology*; D006801:Humans; D011701:PUVA Therapy / Q000009:adverse effects*; D016615:Telomere / Q000187:drug effects*; D059506:Telomere Shortening / Q000187:drug effects; D001616:beta-Galactosidase / Q000032:analysis
chemical_list: D036145:Ginsenosides; C061901:GLB1 protein, human; D001616:beta-Galactosidase; C035054:ginsenoside Rg1