title: High-fat and fructose intake induces insulin resistance, dyslipidemia, and liver steatosis and alters in vivo macrophage-to-feces reverse cholesterol transport in hamsters.
abstract: Reverse cholesterol transport (RCT) promotes the egress of cholesterol from peripheral tissues to the liver for biliary and fecal excretion. Although not demonstrated in vivo, RCT is thought to be impaired in patients with metabolic syndrome, in which liver steatosis prevalence is relatively high. Golden Syrian hamsters were fed a nonpurified (CON) diet and normal drinking water or a high-fat (HF) diet containing 27% fat, 0.5% cholesterol, and 0.25% deoxycholate as well as 10% fructose in drinking water for 4 wk. Compared to CON, the HF diet induced insulin resistance and dyslipidemia, with significantly higher plasma non-HDL-cholesterol concentrations and cholesteryl ester transfer protein activity. The HF diet induced severe liver steatosis, with significantly higher cholesterol and TG levels compared to CON. In vivo RCT was assessed by i.p. injecting ³H-cholesterol labeled macrophages. Compared to CON, HF hamsters had significantly greater ³H-tracer recoveries in plasma, but not HDL. After 72 h, ³H-tracer recovery in HF hamsters was 318% higher in liver and 75% lower in bile (P < 0.01), indicating that the HF diet impaired hepatic cholesterol fluxes. However, macrophage-derived cholesterol fecal excretion was 45% higher in HF hamsters than in CON hamsters. This effect was not related to intestinal cholesterol absorption, which was 89% higher in HF hamsters (P < 0.05), suggesting a possible upregulation of transintestinal cholesterol excretion. Our data indicate a significant increase in macrophage-derived cholesterol fecal excretion in a hamster model of metabolic syndrome, which may not compensate for the diet-induced dyslipidemia and liver steatosis.
authors.affiliation: Physiogenex SAS, Prologue Biotech, Rue Pierre et Marie Curie, Labège-Innopole, France. f.briand@physiogenex.com
authors.forename: François
authors.initials: F
authors.lastname: Briand
authors.forename: Quentin
authors.initials: Q
authors.lastname: Thiéblemont
authors.forename: Elodie
authors.initials: E
authors.lastname: Muzotte
authors.forename: Thierry
authors.initials: T
authors.lastname: Sulpice
mesh_terms: D000818:Animals; D001692:Biological Transport; D002784:Cholesterol / Q000032:analysis / Q000097:blood / Q000378:metabolism*; D053480:Cholesterol Ester Transfer Proteins / Q000097:blood; D006224:Cricetinae; D059305:Diet, High-Fat / Q000009:adverse effects*; D004195:Disease Models, Animal; D050171:Dyslipidemias / Q000209:etiology; D005234:Fatty Liver / Q000209:etiology; D005243:Feces / Q000737:chemistry; D005632:Fructose / Q000009:adverse effects*; D007333:Insulin Resistance; D007700:Kinetics; D008099:Liver / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D008647:Mesocricetus; D024821:Metabolic Syndrome / Q000097:blood / Q000209:etiology / Q000378:metabolism* / Q000503:physiopathology; D014280:Triglycerides / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D053480:Cholesterol Ester Transfer Proteins; D014280:Triglycerides; D005632:Fructose; D002784:Cholesterol