title: The interrelationships between Src, Cav-1 and RhoGD12 in transitional cell carcinoma of the bladder.
abstract: BACKGROUND
  The aim of this current study was to assess the expression and activity of Src family kinases, focal adhesion kinase (FAK), caveolin (Cav-1) and RhoGD12 in bladder cancer.
  
  
  METHODS
  Fifty-eight patients with a new diagnosis of bladder cancer undergoing transurethral resection were included. Immunohistochemical staining was utilised to assess expression of c-Src, dephosphorylated (SrcY(530)), phosphorylated Src (Y(419)), phosphorylated FAK (FAK Y(861)), Cav-1 and RhoGD12. Expression was assessed using the weighted histoscore method.
  
  
  RESULTS
  High expression of dephosphorylated Y(527), phosphorylated Y(416) and phosphorylated FAK Y(861) in the membrane were associated with increased cancer-specific survival (P=0. 01, P=0.001, P=0.008, respectively) and expression of Y(416) in the membrane was an independent factor on multivariate analysis when combined with known clinical parameters (P=0.008, HR 0.288, 95% CI 0.11-0.72).
  
  
  CONCLUSIONS
  These results demonstrate that in contrast to other solid tumours, activation of the Src family members and downstream signalling proteins are associated with a good prognosis in transitional cell carcinoma of the bladder, and activated Src has a positive relationship with RhoGD12.
authors.affiliation: Institute of Cancer, College of MVLS, University of Glasgow, Western Infirmary, Glasgow G11 6NT, UK.
authors.forename: T
authors.initials: T
authors.lastname: Qayyum
authors.forename: G
authors.initials: G
authors.lastname: Fyffe
authors.forename: M
authors.initials: M
authors.lastname: Duncan
authors.forename: P A
authors.initials: PA
authors.lastname: McArdle
authors.forename: M
authors.initials: M
authors.lastname: Hilmy
authors.forename: C
authors.initials: C
authors.lastname: Orange
authors.forename: G
authors.initials: G
authors.lastname: Halbert
authors.forename: M
authors.initials: M
authors.lastname: Seywright
authors.forename: P G
authors.initials: PG
authors.lastname: Horgan
authors.forename: M A
authors.initials: MA
authors.lastname: Underwood
authors.forename: J
authors.initials: J
authors.lastname: Edwards
mesh_terms: D000081247:CSK Tyrosine-Protein Kinase; D002295:Carcinoma, Transitional Cell / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D051242:Caveolin 1 / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004789:Enzyme Activation; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D020730:Guanine Nucleotide Dissociation Inhibitors / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D053208:Kaplan-Meier Estimate; D008875:Middle Aged; D015999:Multivariate Analysis; D060787:Neoplasm Grading; D010766:Phosphorylation; D011379:Prognosis; D011505:Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D001749:Urinary Bladder Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D062947:rho-Specific Guanine Nucleotide Dissociation Inhibitors; D019061:src-Family Kinases
chemical_list: C495540:CAV1 protein, human; D051242:Caveolin 1; D020730:Guanine Nucleotide Dissociation Inhibitors; D062947:rho-Specific Guanine Nucleotide Dissociation Inhibitors; D011505:Protein-Tyrosine Kinases; D000081247:CSK Tyrosine-Protein Kinase; D051416:Focal Adhesion Protein-Tyrosine Kinases; D019061:src-Family Kinases; C000631346:CSK protein, human