title: Increased PARP-1 levels in nuclear matrix isolated from heat shock treated rat liver.
abstract: Poly(ADP-ribose) polymerase-1 (PARP-1), a chromatin-associated enzyme that catalyzes the NAD+-dependent addition of ADP-ribose polymers onto a variety of nuclear proteins, has been shown to be associated with the nuclear matrix. PARP-1 levels in the nuclear matrix vary depending on the matrix isolation method used. The nuclear matrix appears to be the most thermosensitive nuclear structure during heat shock. Here we provide evidence for the extensive translocation of PARP-1 from chromatin to the nuclear matrix during heat shock. This translocation is accompanied by inhibition of PARP activity in the nucleus and elevation of PARP activity in the nuclear matrix. Our data suggest that thermal destabilization of the nuclear matrix is less likely to contribute to the translocation of PARP-1 to the nuclear matrix.
authors.affiliation: Department of Genome Structure and Function, Life Sciences Research Center, Tbilisi, Georgia. giozaal@gmail.com
authors.forename: G
authors.initials: G
authors.lastname: Zaalishvili
authors.forename: E
authors.initials: E
authors.lastname: Zaldastanishvili
authors.forename: M
authors.initials: M
authors.lastname: Karapetian
authors.forename: T
authors.initials: T
authors.lastname: Zaalishvili
mesh_terms: D000818:Animals; D002843:Chromatin / Q000378:metabolism; D018869:Heat-Shock Response; D008099:Liver / Q000378:metabolism*; D008297:Male; D015530:Nuclear Matrix / Q000201:enzymology*; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013696:Temperature
chemical_list: D002843:Chromatin; C499322:Parp1 protein, rat; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases