title: Sez-6 may play an important role in neurite outgrowth through the PKCgamma signaling pathways.
abstract: Seizure-related gene 6 (sez-6) was originally identified in a study of pentylenetetrazole-treated cortical neurons. Further studies on the structure and expression pattern suggested that Sez-6 may play an important role in neuronal development and function. In the present study, PC12 cells were used as a model to investigate the role of Sez-6 in neurite outgrowth. After a period of NGF treatment, the expression of Sez-6 in PC12 cells was increased. When Sez-6 expression was suppressed by the addition of an effective short hairpin RNA (shRNA) plasmid, the neurite outgrowth was significantly inhibited. In addition, we detected the expression level of protein kinase Cgamma (PKCgamma), and found that the PKCgamma protein level was reduced in the differentiated PC12 cells but increased in PC12 cells lacking Sez-6. Taken together, our results indicate that Sez-6 acts on the neurite outgrowth of PC12 cells likely through the PKCgamma signaling pathways.
authors.affiliation: Department of Regenerative Medicine, Tongji University, School of Medicine, Shanghai, 200092, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Youjun
authors.initials: Y
authors.lastname: Wu
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhou
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Shao
authors.forename: Xuan
authors.initials: X
authors.lastname: Kang
authors.forename: Lin
authors.initials: L
authors.lastname: Ma
authors.forename: Mengjie
authors.initials: M
authors.lastname: Li
authors.forename: Lihong
authors.initials: L
authors.lastname: Liu
authors.forename: Hongjun
authors.initials: H
authors.lastname: Shi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D017931:DNA Primers; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000502:physiology*; D016501:Neurites; D016716:PC12 Cells; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction
chemical_list: D017931:DNA Primers; D009419:Nerve Tissue Proteins; C546391:Sez6 protein, rat; C087857:protein kinase C gamma; D011493:Protein Kinase C