title: Erythropoietin reverts cognitive impairment and alters the oxidative parameters and energetic metabolism in sepsis animal model.
abstract: Sepsis is characterized by systemic biochemical alterations including the central nervous system in the early times and cognitive impairment at later times after sepsis induction in the animal model. Recent studies have shown that, besides its hematological activity, erythropoietin (EPO) has cytoprotective effects on various cells and tissues. In order to corroborate elucidating the effects of alternative drugs for sepsis treatment, we evaluated the effects of both acute and chronic EPO treatment on oxidative stress and energetic metabolism in the hippocampus, and cognitive impairment, respectively, after sepsis induction by cecal ligation and perforation (CLP). To this aim, male Wistar rats underwent CLP with "basic support" or sham operation. In the acute treatment, EPO was administered once immediately after CLP induction. The rats were then killed after 6 and 24 h, and the hippocampus was removed for analysis of oxidative stress and energetic metabolism, respectively. Regarding the chronic treatment, EPO was administered once daily until the 4th day after induction. Aversive memory was tested on the 10th day after surgery. It was observed that the acute use of EPO (a single dose) alters the oxidative parameters and energetic metabolism. Chronic use (4 days) reversed cognitive impairment in the sepsis animal model. Mortality rates were attenuated only during chronic treatment.
authors.affiliation: Laboratory of Neurosciences and National Institute for Translational Medicine-INCT-TM, Postgraduate Program in Health Sciences, University of Southern Santa Catarina, Criciúma, SC 88806-000, Brazil.
authors.forename: Clarissa M
authors.initials: CM
authors.lastname: Comim
authors.forename: Omar J
authors.initials: OJ
authors.lastname: Cassol
authors.forename: Igor
authors.initials: I
authors.lastname: Abreu
authors.forename: Thais
authors.initials: T
authors.lastname: Moraz
authors.forename: Larissa S
authors.initials: LS
authors.lastname: Constantino
authors.forename: Francieli
authors.initials: F
authors.lastname: Vuolo
authors.forename: Letícia S
authors.initials: LS
authors.lastname: Galant
authors.forename: Natália
authors.initials: N
authors.lastname: de Rochi
authors.forename: Meline O
authors.initials: MO
authors.lastname: Dos Santos Morais
authors.forename: Giselli
authors.initials: G
authors.lastname: Scaini
authors.forename: Tatiana
authors.initials: T
authors.lastname: Barichello
authors.forename: Emílio L
authors.initials: EL
authors.lastname: Streck
authors.forename: João
authors.initials: J
authors.lastname: Quevedo
authors.forename: Felipe
authors.initials: F
authors.lastname: Dal-Pizzol
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects / Q000502:physiology; D002950:Citrate (si)-Synthase / Q000378:metabolism; D003072:Cognition Disorders / Q000188:drug therapy* / Q000209:etiology; D003402:Creatine Kinase / Q000378:metabolism; D004195:Disease Models, Animal; D004579:Electron Transport / Q000187:drug effects; D045222:Electron Transport Chain Complex Proteins / Q000378:metabolism; D004734:Energy Metabolism / Q000187:drug effects*; D004921:Erythropoietin / Q000494:pharmacology* / Q000627:therapeutic use*; D007266:Inhibition, Psychological; D008026:Ligation / Q000009:adverse effects; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000150:complications / Q000209:etiology / Q000378:metabolism*; D018709:Statistics, Nonparametric; D013997:Time Factors
chemical_list: D045222:Electron Transport Chain Complex Proteins; D004921:Erythropoietin; D002950:Citrate (si)-Synthase; D003402:Creatine Kinase