title: Kinetics of fluoride bioavailability in supernatant saliva and salivary sediment.
abstract: The assessment of the fluoride kinetics in whole saliva as well as in the different salivary phases (supernatant saliva and sediment) is essential for the understanding of fluoride bioavailability.
  
  
  OBJECTIVE
  To assess the fluoride content, provided by sodium fluoride and amine fluoride, in the supernatant saliva and in salivary sediment.
  
  
  METHODS
  Seven trained volunteers were randomly attributed to 2 groups in a cross-over design and brushed their teeth in the morning for 3 min with a product containing either sodium fluoride or amine fluoride. Saliva was collected before, immediately after tooth brushing and 30, 120, and 360 min later and measured. The samples were centrifuged 10 min at 3024 × g. Fluoride content of the supernatant saliva and of the sediment was analysed using a fluoride sensitive electrode. All subjects repeated the study cycles 2 times, and statistical analyses were made using the nonparametric sign test for related samples, the Wilcoxon-Mann-Whitney-test for independent samples.
  
  
  RESULTS
  There was a significant increase in fluoride immediately after tooth brushing in both groups in saliva and sediment. The distribution of fluoride between salivary sediment and supernatant saliva (ratio) varied considerably at the different collection times: decreased from 17.87 in baseline samples of saliva to 0.07 immediately and to 0.86 half an hour after tooth brushing in the sodium fluoride group and from 14.33 to 2.85 and to 3.09 in the amine fluoride group. Furthermore after 120 min and after 360 min after tooth brushing the ratio increased from 17.6 to 31.6 in the sodium fluoride group and from 20.5 to 25.76 in the amine fluoride group. No difference was found in the sediment-supernatant saliva ratio between the sodium fluoride and the amine fluoride groups 360 min after tooth brushing.
  
  
  CONCLUSIONS
  For the assessment of fluoride kinetics in whole saliva it is necessary to pay attention to at least four factors: fluoride formulation, time after fluoride application, fluoride concentration in supernatant saliva and fluoride concentration in salivary sediment. This study was approved by the Ethical Committee of the University of Witten/Herdecke permission 21/2008.
authors.affiliation: Faculty of Health, School of Dentistry, University of Witten/Herdecke, Germany.
authors.forename: E A
authors.initials: EA
authors.lastname: Naumova
authors.forename: T
authors.initials: T
authors.lastname: Sandulescu
authors.forename: C
authors.initials: C
authors.lastname: Bochnig
authors.forename: P
authors.initials: P
authors.lastname: Gaengler
authors.forename: S
authors.initials: S
authors.lastname: Zimmer
authors.forename: W H
authors.initials: WH
authors.lastname: Arnold
mesh_terms: D001682:Biological Availability; D002327:Cariostatic Agents / Q000032:analysis*; D018592:Cross-Over Studies; D003773:Dental Plaque / Q000737:chemistry / Q000382:microbiology* / Q000648:ultrastructure; D005459:Fluorides / Q000008:administration & dosage / Q000032:analysis* / Q000493:pharmacokinetics; D006801:Humans; D017736:Ion-Selective Electrodes; D008855:Microscopy, Electron, Scanning; D012463:Saliva / Q000737:chemistry* / Q000187:drug effects / Q000382:microbiology; D018709:Statistics, Nonparametric
chemical_list: D002327:Cariostatic Agents; D005459:Fluorides