title: Detection of a possible bioterrorism agent, Francisella sp., in a clinical specimen by use of next-generation direct DNA sequencing.
abstract: Deep sequencing detected a potential bioterrorism agent, Francisella tularensis, in a human abscess sample (Iwaki-08) of unknown etiology. Identified single-nucleotide variations suggest that the Iwaki-08 case was associated with Francisella tularensis subsp. holarctica (biovar japonica) but not the highly virulent type A (Francisella tularensis subsp. tularensis).
authors.affiliation: Laboratory of Bacterial Genomics, Pathogen Genomics Center, National Institute of Infectious Diseases, Tokyo, Japan. makokuro@nih.go.jp
authors.forename: Makoto
authors.initials: M
authors.lastname: Kuroda
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Sekizuka
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Shinya
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Takeuchi
authors.forename: Takayuki
authors.initials: T
authors.lastname: Kanno
authors.forename: Tetsutaro
authors.initials: T
authors.lastname: Sata
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Asano
mesh_terms: D000038:Abscess / Q000382:microbiology*; D054045:Biological Warfare Agents; D005604:Francisella tularensis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D014406:Tularemia / Q000175:diagnosis* / Q000382:microbiology
chemical_list: D054045:Biological Warfare Agents