title: Amantichitinum ursilacus gen. nov., sp. nov., a chitin-degrading bacterium isolated from soil.
abstract: A bacterial strain named IGB-41(T) was isolated from a soil sample from an ant hill near Stuttgart, Germany. The strain was Gram-negative, rod-shaped, motile and facultatively anaerobic. Phylogenetic analysis based on 16S rRNA grouped the strain IGB-41(T) within the class Betaproteobacteria into the family Neisseriaceae together with Silvimonas amylolytica NBRC 103189(T), Silvimonas iriomotensis NBRC 103188(T) and Silvimonas terrae KM-45(T) as the closest relatives with sequence similarities of 96.7, 96.6 and 96.1 %, respectively. The G+C content of the genomic DNA was determined to be 61.5 mol% and quinone analysis revealed Q-8 as the only detectable quinone. Major cellular fatty acids were identified as C(16 : 0), summed feature 3 (iso-C(15 : 0) 2-OH and/or C(16 : 1)ω7c) and C(18 : 1)ω7c . Strain IGB-41(T) was unique in harbouring phosphoaminolipids, aminolipids and glycoaminolipids when compared with Silvimonas amylolytica NBRC 103189(T) in polar lipid analysis. On the basis of the physiological, phenotypic and genotypic characteristics of strain IGB-41(T), we suggest that the novel strain should be assigned to a new genus Amantichitinum and novel species Amantichitinum ursilacus. The type species of the genus Amantichitinum is Amantichitinum ursilacus and the type strain is IGB-41(T) (=DSM 23761(T) =CIP 110167(T)).
authors.affiliation: Institute for Interfacial Engineering, University of Stuttgart, Nobelstraße 12, 70569 Stuttgart, Germany.
authors.forename: Karin S
authors.initials: KS
authors.lastname: Moß
authors.affiliation: Institute for Interfacial Engineering, University of Stuttgart, Nobelstraße 12, 70569 Stuttgart, Germany.
authors.forename: Stefan C
authors.initials: SC
authors.lastname: Hartmann
authors.affiliation: Fraunhofer Institute for Interfacial Engineering and Biotechnology, Nobelstraße 12, 70569 Stuttgart, Germany.
authors.forename: Isabell
authors.initials: I
authors.lastname: Müller
authors.affiliation: Fraunhofer Institute for Interfacial Engineering and Biotechnology, Nobelstraße 12, 70569 Stuttgart, Germany.
authors.forename: Christina
authors.initials: C
authors.lastname: Fritz
authors.affiliation: Fraunhofer Institute for Interfacial Engineering and Biotechnology, Nobelstraße 12, 70569 Stuttgart, Germany.
authors.forename: Sven
authors.initials: S
authors.lastname: Krügener
authors.affiliation: Fraunhofer Institute for Interfacial Engineering and Biotechnology, Nobelstraße 12, 70569 Stuttgart, Germany.
authors.forename: Susanne
authors.initials: S
authors.lastname: Zibek
authors.affiliation: Fraunhofer Institute for Interfacial Engineering and Biotechnology, Nobelstraße 12, 70569 Stuttgart, Germany.|Institute for Interfacial Engineering, University of Stuttgart, Nobelstraße 12, 70569 Stuttgart, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Hirth
authors.affiliation: Fraunhofer Institute for Interfacial Engineering and Biotechnology, Nobelstraße 12, 70569 Stuttgart, Germany.
authors.forename: Steffen
authors.initials: S
authors.lastname: Rupp
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005858:Germany; D008969:Molecular Sequence Data; D009346:Neisseriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S