title: Phase imaging flow cytometry using a focus-stack collecting microscope.
abstract: This letter introduces a fluidics-based focus-stack collecting microscope. A microfluidic device transports cells through the focal plane of a microscope, resulting in an efficient method to collect focus stacks of large collections of single cells. Images from the focus stacks are used to reconstruct the quantitative phase of cells with the transport-of-intensity-equation method. Using the phase imaging flow cytometer, we measure three-dimensional shape variations of red blood and leukemia cells.
authors.affiliation: Rowland Institute at Harvard, Harvard University, Cambridge, Massachusetts 02142, USA. gorthi@rowland.harvard.edu
authors.forename: Sai Siva
authors.initials: SS
authors.lastname: Gorthi
authors.forename: Ethan
authors.initials: E
authors.lastname: Schonbrun
mesh_terms: D000465:Algorithms; D004912:Erythrocytes / Q000648:ultrastructure; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D019044:Image Cytometry / Q000295:instrumentation / Q000379:methods*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*