title: Investigation of 1-(4-morpholinophenyl)-3-(4-fluorophenyl)-propenone and 1-(4-morpholinophenyl)-3-(3-fluorophenyl)-propenone as new apoptosis inducers on Spodoptera frugiperda (Sf9) cells.
abstract: The objective of this study was to examine the toxicity of 1-(4-morpholinophenyl)-3-(4-fluorophenyl)-propenone (A) and 1-(4-morpholinophenyl)-3-(3-fluorophenyl)-propenone (B) on Spodoptera frugiperda (Sf9) cells and the mechanism of the toxicity. By cell-based thiazolyl blue tetrazolium bromide (MTT) assay, we found that the IC(50) were 35.45 μM for A and 31.97 μM for B respectively. Formation of apoptotic bodies was observed at 24 h in the treated cells. There was a significant increase of DNA ladders in the treated Sf9 cells compared to controls. In the presence of 50 μM compound A and B for 24 h, ATP content of Sf9 cells reduced by approximately 50%. Compared to the controls, the significant over-expression of caspase-3 in treated cells was measured by caspase-3 activity kit, and the loss of mitochondrial membrane potential (ΔΨm) was detected in apoptosis cells. The results suggested that the two compounds could be identified as new potent cell apoptosis inducers.
authors.affiliation: School of Life Sciences, Sichuan University , qYihuan Road, 610064, Chengdu , PR China.
authors.forename: Xingang
authors.initials: X
authors.lastname: Zhang
authors.forename: Ke
authors.initials: K
authors.lastname: Tao
authors.forename: Taiping
authors.initials: T
authors.lastname: Hou
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D002460:Cell Line; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D015394:Molecular Structure; D009025:Morpholines / Q000633:toxicity*; D018411:Spodoptera
chemical_list: C574513:1-(4-morpholinophenyl)-3-(3-fluorophenyl)propenone; C574514:1-(4-morpholinophenyl)-3-(4-fluorophenyl)propenone; D009025:Morpholines; D000255:Adenosine Triphosphate; D053148:Caspase 3