title: A new whole genome amplification method for studying clonal evolution patterns in malignant colorectal polyps.
abstract: To identify the genetic drivers of colorectal tumorigenesis, we applied array comparative genomic hybridization (aCGH) to 13 formalin-fixed paraffin-embedded (FFPE) samples of early, localized human colon adenocarcinomas arising in high-grade adenomas (so-called "malignant polyps"). These lesions are small and hence the amount of DNA is limited. Additionally, the quality of DNA is compromised due to the fragmentation as a consequence of formalin fixation. To overcome these problems, we optimized a newly developed isothermal whole genome amplification system (NuGEN Ovation® WGA FFPE System). Starting with 100 ng of FFPE DNA, the amplification system produced 4.01 ± 0.29 μg (mean ± standard deviation) of DNA. The excellent quality of amplified DNA was further indicated by a high signal-to-noise ratio and a low derivative log(2) ratio spread. Both, the amount of amplified DNA and aCGH performance were independent of the age of the FFPE blocks and the associated degradation of the extracted DNA. We observed losses of chromosome arms 5q and 18q in the adenoma components of the malignant polyp samples, while the embedded early carcinomas revealed losses of 8p, 17p, and 18, and gains of 7, 13, and 20. Aberrations detected in the adenoma components were invariably maintained in the embedded carcinomas. This approach demonstrates that using isothermally whole genome amplified FFPE DNA is technically suitable for aCGH. In addition to demonstrating the clonal origin of the adenoma and carcinoma part within a malignant polyp, the gain of chromosome arm 20q was an indicator for progression from adenoma to carcinoma.
authors.affiliation: Section of Cancer Genomics, Genetics Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: Daniela
authors.initials: D
authors.lastname: Hirsch
authors.forename: Jordi
authors.initials: J
authors.lastname: Camps
authors.forename: Sudhir
authors.initials: S
authors.lastname: Varma
authors.forename: Ralf
authors.initials: R
authors.lastname: Kemmerling
authors.forename: Mark
authors.initials: M
authors.lastname: Stapleton
authors.forename: Thomas
authors.initials: T
authors.lastname: Ried
authors.forename: Timo
authors.initials: T
authors.lastname: Gaiser
mesh_terms: D000236:Adenoma / Q000235:genetics; D002277:Carcinoma / Q000235:genetics; D002869:Chromosome Aberrations; D002890:Chromosomes, Human, Pair 20; D060965:Clonal Evolution / Q000235:genetics*; D003111:Colonic Polyps / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000235:genetics*; D055028:Comparative Genomic Hybridization; D056915:DNA Copy Number Variations; D004273:DNA, Neoplasm; D006801:Humans
chemical_list: D004273:DNA, Neoplasm