title: Directed evolution of highly selective proteases by using a novel FACS-based screen that capitalizes on the p53 regulator MDM2.
authors.affiliation: Department of Molecular Science and Technology, Division of Applied Chemistry and Biological Engineering, Ajou University, Suwon 443-749, South Korea.
authors.forename: Tae Hyeon
authors.initials: TH
authors.lastname: Yoo
authors.forename: Mark
authors.initials: M
authors.lastname: Pogson
authors.forename: Brent L
authors.initials: BL
authors.lastname: Iverson
authors.forename: George
authors.initials: G
authors.lastname: Georgiou
mesh_terms: D019020:Directed Molecular Evolution / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005434:Flow Cytometry / Q000379:methods*; D057166:High-Throughput Screening Assays; D008958:Models, Molecular; D010447:Peptide Hydrolases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D015202:Protein Engineering; D017434:Protein Structure, Tertiary; D051736:Proto-Oncogene Proteins c-mdm2 / Q000737:chemistry / Q000378:metabolism*; D013379:Substrate Specificity; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D016159:Tumor Suppressor Protein p53; D051736:Proto-Oncogene Proteins c-mdm2; D010447:Peptide Hydrolases