title: Increased proliferative effect of organochlorine compounds on human preadipocytes.
abstract: Chlorinated persistent organic pollutants, commonly referred to as organochlorine compounds (OCs), are chemicals of environmental concern that were mostly used historically as pesticides, solvents, flame retardants, and other applications, though some still continue to be produced. OCs accumulate in adipose tissue because of their hydrophobicity. Evidence suggests that OCs modulate adipose tissue metabolism and could affect its development. At the cellular level, the development of adipose tissue is partly controlled by replication of preadipocytes, which may in turn be modulated by contaminants. The aim of this study was to determine whether exposure to specific OCs and to different concentrations, some mimecting those achieved in human tissues that are exposed to chemicals from the environment, affects human preadipocyte proliferation capacity. Human preadipocytes were exposed to various concentrations (3-500 μM) of highly prevalent OCs (PCBs 77, 153 and DDE) for 48 h. At the end of the exposure period, quantification of cell density was assessed by a cell proliferation ELISA assay. Preadipocyte proliferation significantly increased (~28-72%) in response to most of the concentrations of PCB 153 and DDE as compared to the control. These findings suggest that exposure to some OCs and concentrations increase the proliferative capacity of human preadipocytes.
authors.affiliation: Montfort Hospital Research Institute, Nutrition and Metabolism Research Unit, Montfort Hospital, 713 Montreal Road, Pavilion E, 1st Floor, room 2E132, Ottawa, ON K1K 0T2, Canada. n.chapados@gmail.com
authors.forename: Natalie A
authors.initials: NA
authors.lastname: Chapados
authors.forename: Claudia
authors.initials: C
authors.lastname: Casimiro
authors.forename: Michael A
authors.initials: MA
authors.lastname: Robidoux
authors.forename: François
authors.initials: F
authors.lastname: Haman
authors.forename: Malek
authors.initials: M
authors.lastname: Batal
authors.forename: Jules M
authors.initials: JM
authors.lastname: Blais
authors.forename: Pascal
authors.initials: P
authors.lastname: Imbeault
mesh_terms: D052438:Adipocytes, White / Q000502:physiology*; D049109:Cell Proliferation / Q000187:drug effects*; D003633:Dichlorodiphenyl Dichloroethylene / Q000494:pharmacology; D004785:Environmental Pollutants / Q000494:pharmacology; D006801:Humans; D006843:Hydrocarbons, Chlorinated / Q000494:pharmacology*; D007306:Insecticides / Q000494:pharmacology; D011078:Polychlorinated Biphenyls / Q000494:pharmacology; D050151:Subcutaneous Fat / Q000166:cytology
chemical_list: D004785:Environmental Pollutants; D006843:Hydrocarbons, Chlorinated; D007306:Insecticides; D003633:Dichlorodiphenyl Dichloroethylene; D011078:Polychlorinated Biphenyls; C028451:3,4,3',4'-tetrachlorobiphenyl; C014024:2,4,5,2',4',5'-hexachlorobiphenyl