title: Proliferation capacity, neuronal differentiation potency and microstructures after the differentiation of canine bone marrow stromal cells into neurons.
abstract: We examined the proliferation capacity and neuronal differentiation potency of canine bone marrow stromal cells (BMSCs). In addition, the microstructures of neuron-like cells after neuronal differentiation were observed under a scanning electron microscope. Canine BMSCs grew to confluency at 10.0 ± 2.5 days, and 3.8 ± 2.1 × 10(6) BMSCs were collected in one passage. Approximately 65% of canine BMSCs changed to neuron-like morphology after neuronal differentiation, and nearly all neuron-like cells stained positive against neuron-specific enolase. In addition, microstructures such as the cellular organelles, filaments and growth cones of these cells bore a close resemblance to those of the original mature neurons. These results suggested that canine BMSCs might be capable of differentiating into neurons.
authors.affiliation: Laboratory of Veterinary Surgery, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa, 252-0880, Japan. edamura@brs.nihon-u.ac.jp
authors.forename: Kazuya
authors.initials: K
authors.lastname: Edamura
authors.forename: Koji
authors.initials: K
authors.lastname: Kuriyama
authors.forename: Kenichi
authors.initials: K
authors.lastname: Kato
authors.forename: Rei
authors.initials: R
authors.lastname: Nakano
authors.forename: Kenji
authors.initials: K
authors.lastname: Teshima
authors.forename: Kazushi
authors.initials: K
authors.lastname: Asano
authors.forename: Tsuneo
authors.initials: T
authors.lastname: Sato
authors.forename: Shigeo
authors.initials: S
authors.lastname: Tanaka
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation; D004285:Dogs / Q000502:physiology*; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D008855:Microscopy, Electron, Scanning / Q000662:veterinary; D009474:Neurons / Q000166:cytology*