title: Tandem assembly of the epothilone biosynthetic gene cluster by in vitro site-specific recombination.
abstract: We describe a site-specific recombination-based tandem assembly (SSRTA) method for reconstruction of biological parts in synthetic biology. The system was catalyzed by Streptomyces phage φBT1 integrase, which belongs to the large serine recombinase subfamily. This one-step approach was efficient and accurate, and able to join multiple DNA molecules in vitro in a defined order. Thus, it could have applications in constructing metabolic pathways and genetic networks.
authors.affiliation: Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai 200433, China; Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.forename: Guoping
authors.initials: G
authors.lastname: Zhao
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Ding
mesh_terms: D001435:Bacteriophages / Q000201:enzymology; D034261:Epothilones / Q000096:biosynthesis* / Q000235:genetics*; D053263:Gene Regulatory Networks; D019426:Integrases / Q000378:metabolism; D053858:Metabolic Networks and Pathways; D005810:Multigene Family; D011995:Recombination, Genetic; D013302:Streptomyces / Q000821:virology; D058615:Synthetic Biology / Q000379:methods
chemical_list: D034261:Epothilones; D019426:Integrases