title: Diagnostic serum proteomic analysis in patients with active tuberculosis.
abstract: BACKGROUND
  The diagnosis for smear-negative pulmonary tuberculosis (TB) is very difficult. Proteomic fingerprinting of sera is a potentially useful tool.
  
  
  METHODS
  This study analyzed the results of the proteomic fingerprinting in sera obtained from active TB patients and controls using the surface-enhanced laser desorption ionization time of flight mass spectrometry (SELDI-TOF-MS) and protein-chip technology. The peaks were detected and analyzed, and a diagnostic system was developed. The protein peak was identified using high performance liquid chromatography (HPLC)-tandem matrix-assisted laser desorption/ionization-TOF-MS (MALDI-TOF-MS).
  
  
  RESULTS
  Around 50 protein peaks were found significantly different between the TB patients and the controls (P<0.01). Three protein peaks at m/z 5643, 4486 and 4360 were selected for system classification and the development of a decision model. The model distinguished the TB patients from the controls with a sensitivity of 96.9% and a specificity of 97.8%, respectively. The diagnostic accuracy was up to 97.3%. The one most discrepant protein peak at m/z 5643 seen in sera of active TB patients was identified as orosomucoid.
  
  
  CONCLUSIONS
  A diagnostic system for active TB was developed using mass spectrometry and protein chip technology and required only small-volume serum samples. One potential protein biomarker at m/z 5643 was identified as orosomucoid.
authors.affiliation: Army Tuberculosis Key Laboratory, Institute of Tuberculosis Research, the 309th Hospital of PLA, Beijing 100091, China.
authors.forename: Junxian
authors.initials: J
authors.lastname: Zhang
authors.forename: Xueqiong
authors.initials: X
authors.lastname: Wu
authors.forename: Lilan
authors.initials: L
authors.lastname: Shi
authors.forename: Yan
authors.initials: Y
authors.lastname: Liang
authors.forename: Zhensheng
authors.initials: Z
authors.lastname: Xie
authors.forename: Yourong
authors.initials: Y
authors.lastname: Yang
authors.forename: Zhongxing
authors.initials: Z
authors.lastname: Li
authors.forename: Cuihua
authors.initials: C
authors.lastname: Liu
authors.forename: Fuchuan
authors.initials: F
authors.lastname: Yang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000595:Amino Acid Sequence; D015415:Biomarkers / Q000097:blood; D016172:DNA Fingerprinting / Q000379:methods / Q000592:standards; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D040901:Proteomics / Q000379:methods* / Q000592:standards; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods* / Q000592:standards; D014397:Tuberculosis, Pulmonary / Q000097:blood* / Q000175:diagnosis*; D055815:Young Adult
chemical_list: D015415:Biomarkers