title: [The generation and characteristics of reassortant influenza A virus with H5 hemagglutinin and other genes from the apathogenic virus H6N2].
abstract: The experimental reassortant vaccine strain VN-gull (H5N2) containing H5 hemagglutinin (HA) with a removed polybasic site in the connecting peptide and other genes from the apathogenic H6N2 virus A/gull/Moscow/3100/2006 (gull/M) was obtained using a two-step protocol. At Step 1, the reassortant with HA of A/Vietnam/1203/04-PR8/ CDC-RG and other genes from cold-adapted A/Leningrad/17/47 (VN-Len) viruses was generated due to selection with antibody to H2N2 at 26 degrees C. At Step 2, the reassortant VN-gull was obtained by replacing all genes from Len with those from gull/M due to selection with antibody to H6N2 at 39 degrees C. The reassortant VN-Len was apathogenic and the reassortant VN-gull was weakly virulent in mice. Both gave rise to specific antibodies and 4 weeks after single inoculation they provided complete protection against further challenge with highly pathogenic HSN1 virus A/chicken/Kurgan/3/05 (H5N1) (Ku-Len). The chickens infected with live VN-gull virus showed neither clinical symptoms, nor fecal virus excretion; nevertheless, they gave rise to antibodies and were protected from the further challenge with A/chicken/Kurgan/3/2005. The high yield, safety, and protectivity of VN-Len and Ku-Len made them promising strains for the production of inactivated and live vaccines against H5N1 viruses.
authors.forename: E Iu
authors.initials: EIu
authors.lastname: Boravleva
authors.forename: N F
authors.initials: NF
authors.lastname: Lomakina
authors.forename: E A
authors.initials: EA
authors.lastname: Kropotkina
authors.forename: I A
authors.initials: IA
authors.lastname: Rudneva
authors.forename: S S
authors.initials: SS
authors.lastname: Iamnikova
authors.forename: L G
authors.initials: LG
authors.lastname: Rudenko
authors.forename: V V
authors.initials: VV
authors.lastname: Drygin
authors.forename: A S
authors.initials: AS
authors.lastname: Gambarian
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000276:immunology; D046091:Charadriiformes / Q000276:immunology; D002642:Chick Embryo; D002645:Chickens / Q000276:immunology; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000737:chemistry; D006801:Humans; D053125:Influenza A Virus, H5N2 Subtype / Q000235:genetics / Q000276:immunology; D007252:Influenza Vaccines / Q000235:genetics / Q000276:immunology; D005585:Influenza in Birds / Q000276:immunology / Q000517:prevention & control; D007251:Influenza, Human / Q000276:immunology / Q000517:prevention & control; D051379:Mice; D023421:Models, Animal; D016865:Reassortant Viruses / Q000235:genetics / Q000276:immunology; D013696:Temperature; D014611:Vaccination; D014613:Vaccines, Attenuated / Q000235:genetics / Q000276:immunology; D014779:Virus Replication
chemical_list: D000914:Antibodies, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines; D014613:Vaccines, Attenuated; C109963:hemagglutinin, avian influenza A virus