title: Enhanced productivity of Protein C by recombinant human cells in automated fed-batch cultures.
abstract: Recombinant human kidney cells, 293, were cultivated in serum-free fed-batch cultures for the production of Protein C. By coupling the feeding of concentrated medium to pH control based on established stoichiometric relations, the titer of Protein C increased by more than ten fold as compared to batch culture, even though the total cell concentration increased only by less than two fold. Such a fed-batch culture is a simple system for enhancing the productivity of mammalian cells in culture.
authors.affiliation: Department of Chemical Engineering and Materials Science, University of Minnesota, 55455, Minneapolis, MN, USA.
authors.forename: Y S
authors.initials: YS
authors.lastname: Kyung
authors.forename: W S
authors.initials: WS
authors.lastname: Hu