title: Peritoneal catheter implantation elicits IL-10-producing immune-suppressor macrophages through a MyD88-dependent pathway.
abstract: Catheters are implanted into the peritoneal cavity during the process of peritoneal dialysis. Though these catheters may be effective and beneficial, the impact of catheters on the immune system is poorly understood. Catheters and other devices implanted in the peritoneal cavity elicit a foreign body reaction. However, the immunological consequences of this remain uncharacterized. To model this, catheters were implanted into the peritoneal cavity of healthy mice. Catheter implantation induced rapid cellular changes within the peritoneal cavity. Whereas B-cells and T-cells were reduced, catheter implantation was associated with the rapid expansion of F4/80-low-positive, CD11b-positive macrophages that elaborated IL-10, and suppressed T-cell division and Th1 skewing in co-culture assays. Peritoneal catheter elicited macrophages had increased Jmjd3 but reduced NF-κB activation, and their emergence was MyD88-dependent. Collectively, these studies indicate that foreign body implantation into the peritoneal cavity is associated with the expansion of suppressor macrophages. Whether peritoneal cavity catheter implantation may have systemic immunoregulatory roles remains to be explored.
authors.affiliation: Department of Internal Medicine, University of Texas Southwestern Medical School, Dallas, TX 75390, USA.
authors.forename: So-Youn
authors.initials: SY
authors.lastname: Min
authors.forename: Yuyang
authors.initials: Y
authors.lastname: Fu
authors.forename: Jack
authors.initials: J
authors.lastname: Hutcheson
authors.forename: Tianfu
authors.initials: T
authors.lastname: Wu
authors.forename: Elhaum
authors.initials: E
authors.lastname: Khobahy
authors.forename: Jiankun
authors.initials: J
authors.lastname: Zhu
authors.forename: Kamala
authors.initials: K
authors.lastname: Vanarsa
authors.forename: Yong
authors.initials: Y
authors.lastname: Du
authors.forename: Min-Jung
authors.initials: MJ
authors.lastname: Park
authors.forename: Hyun-Sil
authors.initials: HS
authors.lastname: Park
authors.forename: Ramesh
authors.initials: R
authors.lastname: Saxena
authors.forename: Ho-Youn
authors.initials: HY
authors.lastname: Kim
authors.forename: Chandra
authors.initials: C
authors.lastname: Mohan
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000276:immunology / Q000378:metabolism; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D039481:CD11b Antigen / Q000276:immunology / Q000378:metabolism; D018956:CD5 Antigens / Q000276:immunology / Q000378:metabolism; D002408:Catheters, Indwelling; D002452:Cell Count; D005434:Flow Cytometry; D005549:Foreign-Body Reaction / Q000276:immunology; D006801:Humans; D016753:Interleukin-10 / Q000276:immunology* / Q000378:metabolism; D056484:Jumonji Domain-Containing Histone Demethylases / Q000276:immunology / Q000378:metabolism; D008262:Macrophage Activation / Q000276:immunology; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018613:Microscopy, Confocal; D053594:Myeloid Differentiation Factor 88 / Q000276:immunology* / Q000378:metabolism; D010529:Peritoneal Cavity / Q000166:cytology; D010530:Peritoneal Dialysis; D015398:Signal Transduction / Q000276:immunology*; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism
chemical_list: D000943:Antigens, Differentiation; D039481:CD11b Antigen; D018956:CD5 Antigens; D053594:Myeloid Differentiation Factor 88; C036763:monocyte-macrophage differentiation antigen; D016753:Interleukin-10; D056484:Jumonji Domain-Containing Histone Demethylases; C523761:Kdm6b protein, mouse