title: Highly sensitive detection of platelet-derived growth factor on a functionalized diamond surface using aptamer sandwich design.
abstract: Aptamer-based fluorescence detection of platelet-derived growth factor (PDGF) on a functionalized diamond surface was demonstrated. In this work, a sandwich design based on the ability of PDGF to bind with aptamers at its two available binding sites was employed. It was found that this sandwich design approach significantly increases the fluorescence signal intensity, and thereby a very low detection limit of 4 pM was achieved. The effect of the ionic strength of MgCl(2) buffer solution was also investigated, and the most favourable binding for PDGF-BB occurred at a Mg(2+) concentration of 5.5 mM. Since the aptamers bind to the target PDGF with high affinity, fluorescence detection exhibited high selectivity towards different biomolecules. The high reproducibility of detection was confirmed by performing three cycles of measurements over a period of three days.
authors.affiliation: School of Science and Engineering, Waseda University, 3-4-1 Ohkubo, Shinjuku, Tokyo 169-8555, Japan. ruslinda@toki.waseda.jp
authors.forename: A Rahim
authors.initials: AR
authors.lastname: Ruslinda
authors.forename: Varun
authors.initials: V
authors.lastname: Penmatsa
authors.forename: Yoko
authors.initials: Y
authors.lastname: Ishii
authors.forename: Shinya
authors.initials: S
authors.lastname: Tajima
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kawarada
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D018130:Diamond / Q000737:chemistry*; D057230:Limit of Detection; D010982:Platelet-Derived Growth Factor / Q000032:analysis / Q000737:chemistry*; D011506:Proteins / Q000032:analysis / Q000737:chemistry; D013499:Surface Properties
chemical_list: D052157:Aptamers, Nucleotide; D010982:Platelet-Derived Growth Factor; D011506:Proteins; D018130:Diamond