title: Effects of the recombinant toxin protein rLj-RGD3 in multidrug-resistant human breast carcinoma cells.
abstract: We have previously reported that Lj-RGD3, a novel RGD-toxin protein, was isolated from the buccal gland of Lampetra japonica. The recombinant protein rLj-RGD3 has anti-invasive and anti-adhesive activity in tumor cells (HeLa cells) and endothelial cells (ECV304 cells) in vitro, and inhibits αvβ3, αvβ5, and β1 integrin-mediated adhesion. In this study, we investigated the bioactivity of rLj-RGD3 in the drug-resistant MCF-7/Adr breast carcinoma cell line and drug-sensitive parental line MCF-7, and found that rLj-RGD3 inhibited the growth of both cell lines. Biological function studies revealed that rLj-RGD3 could induce the apoptosis in MCF-7/Adr, which was more prevalent than that in the drug-sensitive parental line MCF-7. In addition, rLj-RGD3 inhibited the adhesion of MCF-7/Adr cells to fibronectin. Furthermore, rLj-RGD3 prevented invasion of MCF-7/Adr cells through an artificial matrigel basement membrane. In summary, rLj-RGD3 may be used as a potential drug in multidrug-resistant breast cancer therapy.
authors.affiliation: College of Life Sciences, Liaoning Normal University, Dalian, China.
authors.forename: Minli
authors.initials: M
authors.lastname: Jin
authors.forename: Jihong
authors.initials: J
authors.lastname: Wang
authors.forename: Rong
authors.initials: R
authors.lastname: Xiao
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Fenfang
authors.initials: F
authors.lastname: Wu
authors.forename: Yue
authors.initials: Y
authors.lastname: Pang
authors.forename: Bo
authors.initials: B
authors.lastname: Feng
authors.forename: Donghui
authors.initials: D
authors.lastname: Yang
authors.forename: Qingwei
authors.initials: Q
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001943:Breast Neoplasms / Q000188:drug therapy*; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D005260:Female; D005353:Fibronectins / Q000378:metabolism; D005397:Fish Venoms / Q000627:therapeutic use*; D006367:HeLa Cells; D006801:Humans; D000922:Immunotoxins / Q000627:therapeutic use*; D009361:Neoplasm Invasiveness / Q000517:prevention & control; D009842:Oligopeptides / Q000627:therapeutic use*
chemical_list: D005353:Fibronectins; D005397:Fish Venoms; D000922:Immunotoxins; D009842:Oligopeptides; C574387:RGD3 protein, Lampetra japonica; C047981:arginyl-glycyl-aspartic acid