title: K, Ca complexes with a sulfonic ligand: structure and DNA-binding properties.
abstract: A novel 4-(2,4-dihydroxybenzylideneamino)benzenesulfonic acid (HL), and its kalium(I), calcium(II) complexes [M(L)n]·2nH(2)O·Cl(n) (M=K(1) n=1, M=Ca(2) n=2), have been prepared and characterized. The crystal and molecular structures of 1 and 2 were determined by single-crystal X-ray diffraction. The interaction of 1, 2 and ligand (L) with calf thymus DNA was investigated by UV-visible (UV-vis), fluorescence and viscosity measurements. Experimental results indicate that 1, 2 and L could bind to DNA via the intercalation mode, and the binding affinity of 1 is stronger than that of 2 and L. The intrinsic binding constants of 1, 2 and L were 5.60×10(5), 6.53×10(5) and 1.44×10(5)M(-1), respectively. The cleavage reaction on plasmid DNA has been monitored by agarose gel electrophoresis. The results indicated that 1 and 2 could cleave pBR322 DNA.
authors.affiliation: National Quality Supervision and Test Center for Paints, Guangdong Testing Institute for Product Quality Supervision, PR China.
authors.forename: Jiahe
authors.initials: J
authors.lastname: Luo
authors.forename: Zhaorong
authors.initials: Z
authors.lastname: Ma
authors.forename: Huang
authors.initials: H
authors.lastname: Liang
authors.forename: Jiwen
authors.initials: J
authors.lastname: Chen
authors.forename: Zhengzhi
authors.initials: Z
authors.lastname: Zeng
mesh_terms: D002118:Calcium / Q000737:chemistry / Q000378:metabolism*; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D008024:Ligands; D008958:Models, Molecular; D015394:Molecular Structure; D010957:Plasmids; D011188:Potassium / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D013451:Sulfonic Acids / Q000737:chemistry / Q000378:metabolism*; D014961:X-Ray Diffraction
chemical_list: D008024:Ligands; D013451:Sulfonic Acids; D004247:DNA; C427203:calf thymus DNA; D011188:Potassium; D002118:Calcium