title: [Role of connective tissue growth factor (CTGF) in proliferation and migration of pancreatic cancer cells].
abstract: OBJECTIVE
  To explore the expression of connective tissue growth factor (CTGF) in pancreatic cancer and its influence on the proliferation and migration of cancer cells.
  
  
  METHODS
  The expression of CTGF in pancreatic cell line PANC-1 cells was analyzed by real-time PCR and in pancreatic carcinoma (50 cases) tissues by immunohistochemistry. The ability of proliferation and migration in vitro of PANC-1 cells was tested by MTT assay, scratch test and Boyden chamber test after the CTGF gene was overexpressed by Ad5-CTGF or silenced with Ad5-siCTGF transfection.
  
  
  RESULTS
  CTGF was overexpressed in both pancreatic cancer cells and tissues. Overxpression of CTGF leads to increased proliferation and migration of PANC-1 cells. The CTGF-transfected PANC-1 cells showed apparent stronger proliferation ability and scratch-repair ability than that of empty vector controls. The results of Boyden chamber test showed that there were 34 cells/field (200× magnificantion) of the CTGF-transfected overexpressing cells, much more than the 11 cells/field of the empty vector control cells; and 6 cells/microscopic field of the Ad5-siCTGF-transfected silenced cells, much less than the 15 cells/field of the control cells.
  
  
  CONCLUSIONS
  CTGF is overexpressed in both pancreatic cancer cells in vitro and in vivo, indicating that it may play an important role in the cell proliferation and migration in pancreatic cancer.
authors.affiliation: Department of Pathology, Chongqing Medical University, Chongqing 400014, China.
authors.forename: Yu-chun
authors.initials: YC
authors.lastname: Bai
authors.forename: Quan
authors.initials: Q
authors.lastname: Kang
authors.forename: Qing
authors.initials: Q
authors.lastname: Luo
authors.forename: Dao-qi
authors.initials: DQ
authors.lastname: Wu
authors.forename: Wei-xia
authors.initials: WX
authors.lastname: Ye
authors.forename: Xue-mei
authors.initials: XM
authors.lastname: Lin
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D055513:Connective Tissue Growth Factor / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D010190:Pancreatic Neoplasms / Q000378:metabolism / Q000473:pathology*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014162:Transfection
chemical_list: C526317:CCN2 protein, human; D011994:Recombinant Proteins; D055513:Connective Tissue Growth Factor