title: [Cytotoxicity of two dental materials on fibroblasts derived from human embryonic stem cells].
abstract: OBJECTIVE
  To investigate the cytotoxicity of calcium hydroxide (CH) and composite resin on fibroblasts derived from human embryo body fibroblasts-H9 (EBf-H9), human dental pulp cells (hDPCs) and immortalized fibroblasts L929; and to evaluate the use of EBf-H9 as a cellular model for cytotoxicity screening of dental materials.
  
  
  METHODS
  The EBf-H9 cells were derived from human embryonic stem cells (H9) via outgrowth of embryonic body (EB); hDPCs were isolated from healthy dental pulp, and identified by immunochemical staining. Cell Counting Kit-8 (CCK-8) assay was applied to analyze the cytotoxicity of CH and composite resin with serial concentrations on the 3 kinds of cells.
  
  
  RESULTS
  Following 24 h and 48 h (or 72 h) post-treatment of CH and composite resin, the viability of L929 cells was significantly lower than that of EBf-H9 and hDPCs (P<0.05), and there was no significant difference between the last two groups (P>0.05).
  
  
  CONCLUSIONS
  Immortalized fibroblasts L929 cells exhibited different response to CH and composite resin compared with EBf-H9 and hDPCs, and the last two cell types were similar to each other. This study indicated that fibroblasts derived from human embryonic stem cells were a potential cellular model instead of traditional immortalized murine cell line for cytotoxicity screening assay.
authors.affiliation: Department of Pediatric dentistry, Peking Unversity School and Hospital of Stomatology, Beijing 100081, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhan
authors.forename: Xiao-ying
authors.initials: XY
authors.lastname: Wang
authors.forename: Sheng-lin
authors.initials: SL
authors.lastname: Li
authors.forename: Xin
authors.initials: X
authors.lastname: Fu
authors.forename: Guang-yan
authors.initials: GY
authors.lastname: Yu
authors.forename: Tong
authors.initials: T
authors.lastname: Cao
authors.forename: He
authors.initials: H
authors.lastname: Liu
mesh_terms: D000818:Animals; D002126:Calcium Hydroxide / Q000633:toxicity*; D002478:Cells, Cultured; D003188:Composite Resins / Q000633:toxicity*; D003764:Dental Materials / Q000633:toxicity*; D003782:Dental Pulp / Q000166:cytology; D053595:Embryonic Stem Cells / Q000166:cytology / Q000187:drug effects*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D008422:Materials Testing; D051379:Mice; D018675:Toxicity Tests
chemical_list: D003188:Composite Resins; D003764:Dental Materials; D002126:Calcium Hydroxide