title: [The effect of basic fibroblast growth factor on the gene expression of syndecan-4 by human periodontal ligament cell in culture].
abstract: OBJECTIVE
  To study the effect of basic fibroblast growth factor (bFGF) on the gene expression of syndecan-4 by human periodontal ligament cell (PDLC) in culture, and discuss the effect of bFGF on human PDLC proliferation and migration.
  
  
  METHODS
  68 adolescent (12-18 years old) health premolar were collected, which were extracted for orthodontic reason. Human PDLC were cultured and stimulated by exogenous bFGF. After cultured 24, 48, 72h, gene expression of syndecan-4 was detected by SYBR green quantitative real time polymerase chain reaction.
  
  
  RESULTS
  The mRNA expression of syndecan-4 in 24 h group increased markedly than that in control group (P < 0.01), expecially in 1.0 ng x mL(-1) group. 1.0 ng x mL(-1) group in 48 h higher than that control group (P < 0.05). 1.0 ng x mL(-1) group in 72h compared with control group was lower (P < 0.05).
  
  
  CONCLUSIONS
  The mRNA expression of syndecan-4 was increased by bFGF at the beginning, but the expression was decreased with the time. The expression of such changes may be one of the important factors which participate in the migration process of PDLC.
authors.affiliation: Dept. of Periodontology, Stomatology Hospital, Jilin University, Changchun 130021, China.
authors.forename: Jiaqing
authors.initials: J
authors.lastname: Yan
authors.forename: Chongtao
authors.initials: C
authors.lastname: Lin
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Shen
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Zhang
mesh_terms: D002478:Cells, Cultured; D016222:Fibroblast Growth Factor 2; D006801:Humans; D010513:Periodontal Ligament; D012333:RNA, Messenger; D053671:Syndecan-4
chemical_list: D012333:RNA, Messenger; D053671:Syndecan-4; D016222:Fibroblast Growth Factor 2