title: Comparative high-performance liquid chromatography enantioseparations on polysaccharide based chiral stationary phases prepared by coating totally porous and core-shell silica particles.
abstract: This article reports comparative high-performance liquid chromatographic separations of enantiomers with chiral stationary phases (CSPs) prepared by coating cellulose tris(4-chloro-3-methylphenylcarbamate) on totally porous and on core-shell type silica of comparable particle diameter. Several interesting observations were made: (1) the selectivity of separation was higher on core-shell type CSP compared to totally porous CSP at comparable content of chiral selector (polysaccharide derivative); (2) much flatter dependence of plate height on the mobile phase flow rate was observed for columns packet with CSP prepared with core-shell silica compared to the ones packed with CSPs prepared with totally porous particles; (3) at low mobile phase flow rates core-shell CSP provided lower resolving ability compared to a commercially available CSP having four times higher content of chiral selector along with higher retention of chiral analytes. However, at high flow rates core-shell type CSP performed similarly or better than the commercial column in regards of plate count (N) and peak resolution (R(s)) per column length and within a given total analysis time. The advantage of CSP prepared with core-shell silica is obvious from the viewpoint of plate numbers and resolution calculated per unit time (i.e. speed of analysis).
authors.affiliation: Institute of Physical and Analytical Chemistry, School of Exact and Natural Sciences, Tbilisi State University, Chavchavadze Ave 3, 0179 Tbilisi, Georgia.
authors.forename: Ketevan
authors.initials: K
authors.lastname: Lomsadze
authors.forename: George
authors.initials: G
authors.lastname: Jibuti
authors.forename: Tivadar
authors.initials: T
authors.lastname: Farkas
authors.forename: Bezhan
authors.initials: B
authors.lastname: Chankvetadze
mesh_terms: D001555:Benzene Derivatives / Q000032:analysis / Q000737:chemistry; D002482:Cellulose / Q000031:analogs & derivatives* / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods*; D048448:Phenylcarbamates / Q000032:analysis*; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D013237:Stereoisomerism
chemical_list: D001555:Benzene Derivatives; D048448:Phenylcarbamates; C552444:cellulose tris(4-chloro-3-methylphenylcarbamate); D012822:Silicon Dioxide; D002482:Cellulose