title: Separating the inseparable: the metabolomic analysis of plant-pathogen interactions.
abstract: Plant-microbe interactions-whether pathogenic or symbiotic-exert major influences on plant physiology and productivity. Analysis of such interactions represents a particular challenge to metabolomic approaches due to the intimate association between the interacting partners coupled with a general commonality of metabolites. We here describe an approach based on co-cultivation of Arabidopsis cell cultures and bacterial plant pathogens to assess the metabolomes of both interacting partners, which we refer to as dual metabolomics.
authors.affiliation: IBERS - Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, UK.
authors.forename: J William
authors.initials: JW
authors.lastname: Allwood
authors.forename: Jim
authors.initials: J
authors.lastname: Heald
authors.forename: Amanda J
authors.initials: AJ
authors.lastname: Lloyd
authors.forename: Royston
authors.initials: R
authors.lastname: Goodacre
authors.forename: Luis A J
authors.initials: LA
authors.lastname: Mur
mesh_terms: D017360:Arabidopsis / Q000378:metabolism* / Q000382:microbiology*; D054884:Host-Pathogen Interactions; D055442:Metabolome; D055432:Metabolomics / Q000379:methods*; D010944:Plants; D017550:Spectroscopy, Fourier Transform Infrared