title: Protective effects of pine bark extract on hexavalent chromium-induced dermatotoxicity in rats.
abstract: The present study investigated the protective effects of pine bark extract (PBE) against hexavalent chromium [Cr(VI)]-induced dermatotoxicity in rats. Skin reactions were evaluated by visual inspection, histopathological changes and oxidative stress parameters. Topical application of Cr(VI) produced a significant increase in the incidence and severity of erythema and edema upon visual inspection. Histopathological examination showed moderate to severe necrosis and desquamation in the epidermis and inflammation and hemorrhage in the dermis. In addition, an increased malondialdehyde (MDA) concentration, and decreased glutathione (GSH), catalase, superoxide dismutase, glutathione-S-transferase (GST) and glutathione reductase of the skin were observed in the Cr(VI) group. On the contrary, concomitant administration with PBE significantly improved Cr(VI)-induced dermatotoxicity, evidenced by a decrease in the incidence and severity of skin irritation and histopathological lesions in a dose-dependent manner. Moreover, PBE treatment reduced MDA concentrations and increased catalase and GST activities in skin tissues, indicating that concomitant administration with PBE effectively prevents Cr(VI)-induced oxidative damage in rats. The results indicate that PBE has a protective effect against Cr(VI)-induced dermatotoxicity and is useful as a protective agent against various dermal lesions induced by oxidative stress.
authors.affiliation: College of Veterinary Medicine, Chonnam National University, Gwangju, South Korea.
authors.forename: In-Chul
authors.initials: IC
authors.lastname: Lee
authors.forename: Sung-Hwan
authors.initials: SH
authors.lastname: Kim
authors.forename: In-Sik
authors.initials: IS
authors.lastname: Shin
authors.forename: Changjong
authors.initials: C
authors.lastname: Moon
authors.forename: Soo-Hyun
authors.initials: SH
authors.lastname: Park
authors.forename: Sung-Ho
authors.initials: SH
authors.lastname: Kim
authors.forename: Seung-Chun
authors.initials: SC
authors.lastname: Park
authors.forename: Hyoung-Chin
authors.initials: HC
authors.lastname: Kim
authors.forename: Jong-Choon
authors.initials: JC
authors.lastname: Kim
mesh_terms: D000818:Animals; D002374:Catalase / Q000032:analysis; D002857:Chromium / Q000633:toxicity*; D004487:Edema / Q000188:drug therapy; D004890:Erythema / Q000188:drug therapy; D005419:Flavonoids / Q000494:pharmacology*; D005978:Glutathione / Q000032:analysis; D005980:Glutathione Reductase / Q000032:analysis; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D018384:Oxidative Stress / Q000187:drug effects*; D028223:Pinus / Q000737:chemistry*; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000187:drug effects* / Q000473:pathology; D013482:Superoxide Dismutase / Q000032:analysis
chemical_list: D005419:Flavonoids; D010936:Plant Extracts; D020011:Protective Agents; D002857:Chromium; C074702:chromium hexavalent ion; D008315:Malondialdehyde; C024070:pycnogenols; D002374:Catalase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005978:Glutathione