title: Algorithm proposal based on the C. Diff Quik Chek Complete ICT device for detecting Clostridium difficile infection.
abstract: OBJECTIVE
  To assess a new immunochromatography (ICT) test that detects glutamate dehydrogenase (GDH) antigen and Clostridium difficile toxin A/B simultaneously, and to propose an algorithm for the diagnosis of C. difficile infection (CDI) based on this test.
  
  
  METHODS
  We analysed 970 stool samples. Discrepant results between GDH and toxin A/B were resolved using toxigenic culture as the reference.
  
  
  RESULTS
  This test enabled us to obtain a conclusive result in <30min in 93.8% of the samples. Among the discrepant results (GDH (+)/Toxin A/B (-)), 41.7% (25/60) were found to be toxigenic C. difficile by toxigenic culture.
  
  
  CONCLUSIONS
  This test has a high sensitivity and specificity for the diagnosis of CDI.
authors.affiliation: Servicio de Microbiología, Hospital Universitario 12 de Octubre, Madrid, Spain. morellana.hdoc@salud.madrid.org
authors.forename: M A Angeles
authors.initials: MA
authors.lastname: Orellana-Miguel
authors.forename: Adela
authors.initials: A
authors.lastname: Alcolea-Medina
authors.forename: Laura
authors.initials: L
authors.lastname: Barrado-Blanco
authors.forename: Joaquín
authors.initials: J
authors.lastname: Rodriguez-Otero
authors.forename: Fernando
authors.initials: F
authors.lastname: Chaves-Sánchez
mesh_terms: D000465:Algorithms; D000942:Antigens, Bacterial / Q000032:analysis; D001399:Azure Stains; D002846:Chromatography, Affinity / Q000295:instrumentation; D003015:Clostridium Infections / Q000175:diagnosis*; D005969:Glutamate Dehydrogenase / Q000276:immunology; D006801:Humans; D008751:Methylene Blue; D014966:Xanthenes
chemical_list: D000942:Antigens, Bacterial; D001399:Azure Stains; C067461:Diff Quik; D014966:Xanthenes; D005969:Glutamate Dehydrogenase; D008751:Methylene Blue