title: [Effect of CD8+ effector T cells on the hematopoiesis pathway damage in the patients with severe aplastic anemia].
abstract: OBJECTIVE
  To investigate the quantity and the pathway to damage hematopoietic cells of CD8+CD25+ and CD8+ HLA-DR+ effector T cells in peripheral blood (PB) of severe aplastic anemia(SAA) patients and explore the immunopathogenesis of SAA.
  
  
  METHODS
  The quantity of CD8+ CD25+ and CD8+ HLA-DR+ cells in PB and the expressions of perforin, granzyme B, tumor necrosis factor-beta (TNF-beta) and FasL in 29 SAA (14 untreated and 15 recovered) patients and 12 normal controls were analyzed by flow cytometry.
  
  
  RESULTS
  The fraction of CD8+ CD25+ T cells in CD8+ T cells was (3.67 +/- 2.58)% in untreated SAA patients, (5.19 +/- 4. 29)% in recovered patients and (4.84 +/- 2.31)% in normal controls, and that of CD8+ CD25+ T cells in CD3+ cells in the three groups was (2.25 +/- 1.35)%, (2.98 +/- 1.35)% and (2.11 +/- 1.88)%, respectively. They had no statistic difference among the 3 groups (P >0.05). The fraction of CD8+ HLA-DR+ T cells in CD8+ T cells was (39.30 +/- 8.13)% in untreated patients, which was significantly higher than that in recovered patients [(20.65 +/- 5.38)%] and controls [(18.34 +/- 6.68)%] (P<0.001), while there was no statistic difference between the latter two groups (P>0.05). CD8+ HLA-DR+ T cells in CD3+ cells was (27.81 +/- 7.10)% in untreated group, which was significantly higher than that of recovered group [(12.02 +/- 3.03)%] and controls [(8.50 +/-2.33)%] (P<0.01). And that in recovered group was higher than that in control group (P<0.05). The expressions of perforin, granzyme B, TNF-beta and FasL of CD8+ HLA-DR+ T cells in untreated group were 8.51%, 96.08%, 72.11% and 94.25% respectively, which were higher than those in recovered group (1.78%, 85.20%, 34.38% and 51.20%) and controls (1.86%, 82.09% ,17.92% and 32.91%). There was no statistic difference between recovered patients and controls (P>0.05).
  
  
  CONCLUSIONS
  There were elevated quantity of CD8+ HLA-DR+ T cells and high expressions of perforin, granzyme B, TNF-beta and FasL in SAA, which might contribute to the bone marrow failure.
authors.affiliation: Department of Hematology & Oncology,Tianjin Medical University General Hospital, Tianjin 300052, China.
authors.forename: Le
authors.initials: L
authors.lastname: Feng
authors.forename: Rong
authors.initials: R
authors.lastname: Fu
authors.forename: Hua-quan
authors.initials: HQ
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Chun-yan
authors.initials: CY
authors.lastname: Liu
authors.forename: Li-juan
authors.initials: LJ
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.forename: Hong-lei
authors.initials: HL
authors.lastname: Wang
authors.forename: Tian
authors.initials: T
authors.lastname: Zhang
authors.forename: Er-bao
authors.initials: EB
authors.lastname: Ruan
authors.forename: Yong
authors.initials: Y
authors.lastname: Liang
authors.forename: Wen
authors.initials: W
authors.lastname: Qu
authors.forename: Guo-jin
authors.initials: GJ
authors.lastname: Wang
authors.forename: Yu-hong
authors.initials: YH
authors.lastname: Wu
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Xiao-ming
authors.initials: XM
authors.lastname: Wang
authors.forename: Jia
authors.initials: J
authors.lastname: Song
authors.forename: Jing
authors.initials: J
authors.lastname: Guan
authors.forename: Li-min
authors.initials: LM
authors.lastname: Xing
authors.forename: Zong-hong
authors.initials: ZH
authors.lastname: Shao
mesh_terms: D000293:Adolescent; D000328:Adult; D000741:Anemia, Aplastic / Q000097:blood* / Q000378:metabolism* / Q000473:pathology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology; D016022:Case-Control Studies; D002648:Child; D053222:Fas Ligand Protein / Q000378:metabolism; D005260:Female; D053804:Granzymes / Q000378:metabolism; D006801:Humans; D018655:Lymphocyte Count; D008233:Lymphotoxin-alpha / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D054353:Perforin / Q000378:metabolism; D055815:Young Adult
chemical_list: C505661:FASLG protein, human; D053222:Fas Ligand Protein; D008233:Lymphotoxin-alpha; D054353:Perforin; D053804:Granzymes