title: [Automated ribotyping of Salmonella and Staphylococcus aureus in food poisoning of Guangdong province].
abstract: OBJECTIVE
  To understand the genetic polymorphism of Salmonella and Staphylococcus aureus in Guangdong province, as well as to explore methods for identifying and tracing the source of these two foodborne pathogens.
  
  
  METHODS
  Using the automated ribotyping system, two foodborne pathogens were tested with either EcoR I or Pvu II restriction enzymes. BioNumerics software was then applied for image analysis, database establishment and other corresponding analysis.
  
  
  RESULTS
  Digestion of 32 Salmonella isolates with Pvu II yielded 19 different ribotypes, and digestion of 14 Salmonella isolates with EcoR I yielded 2 different ribotypes. Staphylococcus aureus isolates showed greater genetic diversity, whereas EcoR I digestion of 49 different isolates yielded 31 different ribotypes.
  
  
  CONCLUSIONS
  Unique Salmonella and Staphylococcus aureus isolates could be identified through ribotyping. Although Salmonella serotyping and ribotyping were not strongly correlated, the combination of both restriction enzymes could be used to more effectively identify the genetic relationship among different strains as well as the source of food poisoning. Thus, not only could the genetic relationships amongst the different strains be inferred through ribotyping skills, the source of food poisoning and mode of transmission could also be determined under the use of this method.
authors.affiliation: Institute of Pathogenic Microorganisms, Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 510300, China. gzhdm@21cn.com
authors.forename: Dong-mei
authors.initials: DM
authors.lastname: He
authors.forename: Hai-ming
authors.initials: HM
authors.lastname: Zhu
authors.forename: Wei-dong
authors.initials: WD
authors.lastname: Lai
authors.forename: Hai-yan
authors.initials: HY
authors.lastname: Wang
authors.forename: Bi-xia
authors.initials: BX
authors.lastname: Ke
authors.forename: Hai-ling
authors.initials: HL
authors.lastname: Tan
authors.forename: Bo-sheng
authors.initials: BS
authors.lastname: Li
authors.forename: Xiao-ling
authors.initials: XL
authors.lastname: Deng
authors.forename: Chang-wen
authors.initials: CW
authors.lastname: Ke
mesh_terms: D057205:Automation, Laboratory; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D004196:Disease Outbreaks; D005517:Foodborne Diseases / Q000382:microbiology*; D006801:Humans; D021521:Ribotyping / Q000379:methods*; D012475:Salmonella / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012703:Serotyping; D013211:Staphylococcus aureus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial