title: Avidin-biotin technology : preparation of avidin conjugates.
abstract: The second step required in the application of avidin-biotin technology is to prepare an appropriate avidin-associated probe or probes for the desired application (for general reviews, see refs. 1 and 2). For example, a fluorescent form of avidin can be used for fluorescence microscopy, fluorescence-activated cell sorting, and in some cases, for immunoassay. Like-wise, an avidin-enzyme conjugate can be used for immunoblotting, immunoassay, light microscopy, and in some cases, electron microscopy. An immobilized form of avidin can be used for isolation purposes (see Note 1).
authors.affiliation: Department of Biophysics, The Weizmann Institute of Science, Rehovot, Israel.
authors.forename: E A
authors.initials: EA
authors.lastname: Bayer
authors.forename: M
authors.initials: M
authors.lastname: Wilchek