title: [Expression of MT1-MMP and RECK protein in human gastric carcinoma].
abstract: OBJECTIVE
  To examine the expression of membrane-type-1 matrix metalloproteinase (MT1-MMP) and reversion-inducing cysteine-rich protein with Kazal motifs (RECK) in gastric carcinoma, and investigate its clinical significance, at the same time analyze the correlation between MT1-MMP and RECK expression.
  
  
  METHODS
  MT1-MMP and RECK expression in surgically resected tissue samples of gastric carcinoma was examined by immunohistochemical method (two-step method) , and its correlation with clinicopathological factors was analyzed.
  
  
  RESULTS
  Among the 44 gastric carcinoma samples, 37 (84.1%) were stained positive for MT1-MMP, and 31 (70.5%) for RECK. The expression of MT1-MMP was much higher in poorly differentiated gastric carcinoma samples than moderately and well-differentiated samples (P = 0.015). The expression level of MT1-MMP was associated with invasive depth of tumor cells (P = 0.007), but no difference between sex and lymph node metastasis. On the contrary, the well-differentiated samples showed higher expression of RECK than poorly and moderately differentiated gastric carcinoma samples (P = 0.006). The expression level of RECK did not correlate with sex, lymph node metastasis and invasive depth of tumor cells. RECK expression showed no relation to MT1-MMP expression in the gastric carcinoma.
  
  
  CONCLUSIONS
  Overexpression of MT1-MMP in gastric carcinoma may play an important role during tumor differentiation and metastasis, the RECK protein may have positive effects on the tumor differentiation.
authors.affiliation: Jiangsu Cancer Hospital, Nanjing 210009, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Shen
authors.forename: Ma-Qing
authors.initials: MQ
authors.lastname: Zheng
authors.forename: Xin-Yu
authors.initials: XY
authors.lastname: Xu
authors.forename: Fu-Gen
authors.initials: FG
authors.lastname: Mo
authors.forename: Tong
authors.initials: T
authors.lastname: Zhang
authors.forename: Ji-Feng
authors.initials: JF
authors.lastname: Feng
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002277:Carcinoma / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005260:Female; D058851:GPI-Linked Proteins / Q000235:genetics* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D053511:Matrix Metalloproteinase 14 / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: D058851:GPI-Linked Proteins; C115460:RECK protein, human; C507086:MMP14 protein, human; D053511:Matrix Metalloproteinase 14