title: DNA ploidy measurement in oral leukoplakia: different results between flow and image cytometry.
abstract: The estimated prevalence of oral leukoplakia is worldwide approximately 2%, with an annual malignant transformation rate of approximately 1%. The aim of the present study was to evaluate the possible contribution of ploidy measurement to the prediction of the clinical course, in a well defined cohort of patients with oral leukoplakia. Ploidy was measured by both flow cytometry (FCM-DNA) and image cytometry (ICM-DNA) and we focussed on the comparison of the two different techniques to determine ploidy. A total of 41 patients have been included, with a mean age of 59 years (range 36-78 years). With FCM-DNA, three lesions were aneuploid, with ICM-DNA, 19 lesions were aneuploid. DNA ploidy was compared with clinicopathological and patients parameters. There were no statistically significant differences between DNA ploidy and any patient factor with both FCM-DNA and ICM-DNA. Using FCM-DNA, DNA aneuploid lesions showed statistically significant more dysplasia (p=0.04) than diploid lesions. Furthermore, DNA aneuploid lesions were more frequently encountered at high-risk locations (p=0.03) as being determined with FCM-DNA. These relations were not found when DNA ploidy was determined with ICM-DNA.
authors.affiliation: Department of Oral and Maxillofacial Surgery and Oral Pathology, VU University Medical Center (VUmc)/Academic Centre for Dentistry Amsterdam, The Netherlands. 5806399@ student.acta.nl
authors.forename: E R E A
authors.initials: ER
authors.lastname: Brouns
authors.forename: E
authors.initials: E
authors.lastname: Bloemena
authors.forename: J A M
authors.initials: JA
authors.lastname: Belien
authors.forename: M A M
authors.initials: MA
authors.lastname: Broeckaert
authors.forename: I H A
authors.initials: IH
authors.lastname: Aartman
authors.forename: I
authors.initials: I
authors.lastname: van der Waal
mesh_terms: D000328:Adult; D000368:Aged; D000782:Aneuploidy; D004273:DNA, Neoplasm / Q000235:genetics*; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D019044:Image Cytometry / Q000379:methods*; D007972:Leukoplakia, Oral / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D012189:Retrospective Studies
chemical_list: D004273:DNA, Neoplasm