title: Deracemization of unnatural amino acid: homoalanine using D-amino acid oxidase and ω-transaminase.
abstract: A deracemization method was developed to generate optically pure L-homoalanine from racemic homoalanine using D-amino acid oxidase and ω-transaminase. A whole cell reaction using a biphasic system converted 500 mM racemic homoalanine to 485 mM L-homoalanine (>99% ee).
authors.affiliation: School of Biotechnology, Yeungnam University, Gyeongbuk, 712-749, South Korea.
authors.forename: Young-Man
authors.initials: YM
authors.lastname: Seo
authors.forename: Sam
authors.initials: S
authors.lastname: Mathew
authors.forename: Han-Seop
authors.initials: HS
authors.lastname: Bea
authors.forename: Yong-Ho
authors.initials: YH
authors.lastname: Khang
authors.forename: Sang-Hyeup
authors.initials: SH
authors.lastname: Lee
authors.forename: Byung-Gee
authors.initials: BG
authors.lastname: Kim
authors.forename: Hyungdon
authors.initials: H
authors.lastname: Yun
mesh_terms: D000613:Aminobutyrates / Q000737:chemistry* / Q000378:metabolism; D003605:D-Amino-Acid Oxidase / Q000378:metabolism*; D004926:Escherichia coli / Q000737:chemistry / Q000201:enzymology; D007700:Kinetics; D013237:Stereoisomerism; D000637:Transaminases / Q000378:metabolism*
chemical_list: D000613:Aminobutyrates; C012223:alpha-aminobutyric acid; D003605:D-Amino-Acid Oxidase; D000637:Transaminases