title: Proteomic analysis of Plasmodium in the mosquito: progress and pitfalls.
abstract: Here we discuss proteomic analyses of whole cell preparations of the mosquito stages of malaria parasite development (i.e. gametocytes, microgamete, ookinete, oocyst and sporozoite) of Plasmodium berghei. We also include critiques of the proteomes of two cell fractions from the purified ookinete, namely the micronemes and cell surface. Whereas we summarise key biological interpretations of the data, we also try to identify key methodological constraints we have met, only some of which we were able to resolve. Recognising the need to translate the potential of current genome sequencing into functional understanding, we report our efforts to develop more powerful combinations of methods for the in silico prediction of protein function and location. We have applied this analysis to the proteome of the male gamete, a cell whose very simple structural organisation facilitated interpretation of data. Some of the in silico predictions made have now been supported by ongoing protein tagging and genetic knockout studies. We hope this discussion may assist future studies.
authors.affiliation: The Centre for Bioinformatics, Department of Life Sciences, Imperial College, London SW7 2AZ.
authors.forename: M N
authors.initials: MN
authors.lastname: Wass
authors.forename: R
authors.initials: R
authors.lastname: Stanway
authors.forename: A M
authors.initials: AM
authors.lastname: Blagborough
authors.forename: K
authors.initials: K
authors.lastname: Lal
authors.forename: J H
authors.initials: JH
authors.lastname: Prieto
authors.forename: D
authors.initials: D
authors.lastname: Raine
authors.forename: M J E
authors.initials: MJ
authors.lastname: Sternberg
authors.forename: A M
authors.initials: AM
authors.lastname: Talman
authors.forename: F
authors.initials: F
authors.lastname: Tomley
authors.forename: J
authors.initials: J
authors.lastname: Yates
authors.forename: R E
authors.initials: RE
authors.lastname: Sinden
mesh_terms: D000818:Animals; D009033:Culicidae / Q000469:parasitology*; D005260:Female; D007303:Insect Vectors / Q000469:parasitology*; D008018:Life Cycle Stages; D008288:Malaria / Q000469:parasitology*; D008297:Male; D010962:Plasmodium berghei / Q000254:growth & development* / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D015800:Protozoan Proteins / Q000378:metabolism*
chemical_list: D015800:Protozoan Proteins