title: [Occurrence rate of Helicobacter pylori iceA alleles in infected patient in the Białystok Province].
abstract: Intensity of gastric mucosa and/or duodenal inflammation and their clinical consequences in response to Helicobacter pylori infection depend on a given strain virulence. A receptor protein IceA, induced by contact with epithelium gene A, is one of important proteins in the process of infection. Examinations of iceA genotype have show that the rate of occurrence of H. pylori strains containing iceA1 or iceA2 is differentiated in various regions of the world.
  
  
  OBJECTIVE
  The aim of the study was the evaluation of H. pylori iceA alleles occurrence in infected children and adults, inhabitants of the Bialystok Province regarding the place of living (country, town).
  
  
  METHODS
  H. pylori genotyping was carried out on the basis of the analyses bacterial gene iceA, and allelo-specific PCR were used to determine the former gene and two variants of the gene--iceA1 and iceA2.
  
  
  RESULTS
  Out of 50 analyzed genotypes of H. pylori , the presence of iceA gene was observed in 47 strains of bacteria. iceA allele was presented in 25 isolates of examined bacteria DNA (53.2%) while iceA2--in 29 (61.7%). No iceA alleles were isolated from 3 DNA isolates (6%) whereas in 7 examined isolates (14.0%), the coexistence of both iceA alleles (iceA1 and iceA2) was observed. After excluding isolates of H. pylori DNA with coexisting iceA1 and iceA2 (7 persons), it was stated that iceA1 allele occurred in 19 examined isolates (45.0%) and iceA2--in 23 (55.0%).
  
  
  CONCLUSIONS
  The occurrence of H. pylori strains of iceA2 genotype was more frequent in adults (59.1%). The occurrence of H. pylori strains of iceA2 genotype was more frequent in persons living in the country and town (52.4% and 54.5%, respectively).
authors.affiliation: Zakład Pieleniarstwa Pediatrycznego Uniwersytetu Medycznego w Bialymstoku.
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Maciorkowska
authors.forename: Izabela
authors.initials: I
authors.lastname: Roszko
authors.forename: Maciej
authors.initials: M
authors.lastname: Kaczmarski
authors.forename: Oksana
authors.initials: O
authors.lastname: Kowalczuk
authors.forename: Lech
authors.initials: L
authors.lastname: Chyczewski'
mesh_terms: D000328:Adult; D001426:Bacterial Proteins / Q000302:isolation & purification*; D002648:Child; D002908:Chronic Disease; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D016481:Helicobacter Infections / Q000175:diagnosis* / Q000453:epidemiology* / Q000235:genetics; D016480:Helicobacter pylori / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D011044:Poland / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012307:Risk Factors; D012424:Rural Population / Q000706:statistics & numerical data; D014505:Urban Population / Q000706:statistics & numerical data
chemical_list: D001426:Bacterial Proteins; C408644:IceA2 protein, Helicobacter pylori; C405087:iceA1 protein, Helicobacter pylori