title: Sequence analysis of the Bs-Ag1 gene of Baylisascaris schroederi from the giant panda and an evaluation of the efficacy of a recombinant Baylisascaris schroederi Bs-Ag1 antigen in mice.
abstract: The Baylisascaris schroederi infection rate among wild giant pandas may reach over 50% or even 100%, making it one of the leading causes of death from primary or secondary infection in wild populations. Until now, little was known about how protective immunity to B. schroederi infection could be achieved. The present study was conducted to evaluate the immunogenicity and protective efficacy of recombinant Bs-Ag1 from B. schroederi, by cloning the full-length Bs-Ag1 gene of B. schroederi and expressing it in a heterologous host, Escherichia coli BL21. In mice vaccinated with rBs-Ag1 coupled with Freund's complete adjuvant (FCA), there was a significant reduction (69.2%) in the recovery of challenged B. schroederi L3 compared with either nonvaccinated controls or mice vaccinated with FCA alone. Our study supports the use of Bs-Ag1 as a potential candidate for vaccination against B. schroederi infection and provides basic data for further vaccination trials with mixtures of antigens (with Bs-Ag2 and Bs-Ag3) to B. schroederi.
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, China.
authors.forename: Guangzhi
authors.initials: G
authors.lastname: He
authors.forename: Sijie
authors.initials: S
authors.lastname: Chen
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Yubo
authors.initials: Y
authors.lastname: Yan
authors.forename: Zhihe
authors.initials: Z
authors.lastname: Zhang
authors.forename: Desheng
authors.initials: D
authors.lastname: Li
authors.forename: Hua
authors.initials: H
authors.lastname: Yu
authors.forename: Yue
authors.initials: Y
authors.lastname: Xie
authors.forename: Chengdong
authors.initials: C
authors.lastname: Wang
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Gu
authors.forename: Shuxian
authors.initials: S
authors.lastname: Wang
authors.forename: Xuerong
authors.initials: X
authors.lastname: Peng
authors.forename: Guangyou
authors.initials: G
authors.lastname: Yang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D017191:Ascaridida Infections / Q000517:prevention & control; D001199:Ascaridoidea / Q000235:genetics* / Q000276:immunology* / Q000472:pathogenicity; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D005620:Freund's Adjuvant / Q000276:immunology; D015801:Helminth Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D017422:Sequence Analysis, DNA; D001503:Ursidae / Q000469:parasitology*; D014611:Vaccination
chemical_list: D000947:Antigens, Helminth; D018076:DNA, Complementary; D015801:Helminth Proteins; D011993:Recombinant Fusion Proteins; D005620:Freund's Adjuvant