title: Effect of magnetic nanoparticles on apoptosis and cell cycle induced by wogonin in Raji cells.
abstract: Traditional Chinese medicine is gradually becoming a new source of anticancer drugs. One such example is wogonin, which is cytotoxic to various cancer cell lines in vitro. However, due to its low water solubility, wogonin is restricted to clinical administration. Recently, the application of drug-coated magnetic nanoparticles (MNPs) to increase water solubility of the drug and to enhance its chemotherapeutic efficiency has attracted much attention. In this study, wogonin was conjugated with the drug delivery system of MNPs by mechanical absorption polymerization to fabricate wogonin-loaded MNPs. It was demonstrated that MNPs could strengthen wogonin-induced cell inhibition, apoptosis, and cell cycle arrest in Raji cells by methylthiazol tetrazolium assay, flow cytometer assay, and nuclear 4',6-diamidino-2-phenylindole staining. Furthermore, the molecular mechanisms of these phenomena were explored by western blot, in which the protein levels of caspase 8 and caspase 3 were increased significantly while those of survivin and cyclin E were decreased significantly in wogonin-MNPs group. These findings suggest that the combination of wogonin and MNPs provides a promising strategy for lymphoma therapy.
authors.affiliation: Department of Hematology and Oncology, Zhongda Hospital, Medical School, Southeast University, Nanjing, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Haijun
authors.initials: H
authors.lastname: Zhang
authors.forename: Baoan
authors.initials: B
authors.lastname: Chen
authors.forename: Guohua
authors.initials: G
authors.lastname: Xia
authors.forename: Shuai
authors.initials: S
authors.lastname: Wang
authors.forename: Jian
authors.initials: J
authors.lastname: Cheng
authors.forename: Zeye
authors.initials: Z
authors.lastname: Shao
authors.forename: Chong
authors.initials: C
authors.lastname: Gao
authors.forename: Wen
authors.initials: W
authors.lastname: Bao
authors.forename: Liang
authors.initials: L
authors.lastname: Tian
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Ren
authors.forename: Peipei
authors.initials: P
authors.lastname: Xu
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Cai
authors.forename: Ran
authors.initials: R
authors.lastname: Liu
authors.forename: Xuemei
authors.initials: X
authors.lastname: Wang
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D053181:Caspase 8 / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D019927:Cyclin E / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology; D044950:Flavanones / Q000737:chemistry / Q000494:pharmacology*; D005434:Flow Cytometry; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000378:metabolism; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D000077022:Survivin
chemical_list: C107489:BIRC5 protein, human; D019927:Cyclin E; D004365:Drugs, Chinese Herbal; D044950:Flavanones; D051034:Inhibitor of Apoptosis Proteins; D058185:Magnetite Nanoparticles; D000077022:Survivin; D053148:Caspase 3; D053181:Caspase 8; C085514:wogonin