title: Plant γH2AX foci are required for proper DNA DSB repair responses and colocalize with E2F factors.
abstract: Cellular responses to DNA double-strand breaks (DSBs) are linked in mammals and yeasts to the phosphorylated histones H2AX (γH2AX) repair foci which are multiproteic nuclear complexes responsible for DSB sensing and signalling. However, neither the components of these foci nor their role are yet known in plants. In this paper, we describe the effects of γH2AX deficiency in Arabidopsis thaliana plants challenged with DSBs in terms of genotoxic sensitivity and E2F-mediated transcriptional responses. We further establish the existence, restrictive to the G1/S transition, of specific DSB-induced foci containing tobacco E2F transcription factors, in both A. thaliana roots and BY-2 tobacco cells. These E2F foci partially colocalize with γH2AX foci while their formation is ataxia telangiectasia mutated (ATM)-dependent, requires the E2F transactivation domain with its retinoblastoma-binding site and is optimal in the presence of functional H2AXs. Overall, our results unveil a new interplay between plant H2AX and E2F transcriptional activators during the DSB response.
authors.affiliation: Institut de Biologie Moléculaire des Plantes, laboratoire propre du CNRS, (UPR 2357) conventionné avec l'Université de Strasbourg 12, rue du Général Zimmer, 67084 Strasbourg Cedex, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Lang
authors.affiliation: Institut de Biologie Moléculaire des Plantes, laboratoire propre du CNRS, (UPR 2357) conventionné avec l'Université de Strasbourg 12, rue du Général Zimmer, 67084 Strasbourg Cedex, France.
authors.forename: Ondrej
authors.initials: O
authors.lastname: Smetana
authors.affiliation: Laboratorio de Biología Molecular de Plantas Unidad Académica de Biología Experimental Universidad Autónoma de Zacatecas, Av. Revolución S/N Col. Tierra y Libertad CP, 98615 Guadalupe, Zacatecas, México.
authors.forename: Lenin
authors.initials: L
authors.lastname: Sanchez-Calderon
authors.affiliation: Institut de Biologie Moléculaire des Plantes, laboratoire propre du CNRS, (UPR 2357) conventionné avec l'Université de Strasbourg 12, rue du Général Zimmer, 67084 Strasbourg Cedex, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Lincker
authors.affiliation: Institut de Biologie Moléculaire des Plantes, laboratoire propre du CNRS, (UPR 2357) conventionné avec l'Université de Strasbourg 12, rue du Général Zimmer, 67084 Strasbourg Cedex, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Genestier
authors.affiliation: Institut de Biologie Moléculaire des Plantes, laboratoire propre du CNRS, (UPR 2357) conventionné avec l'Université de Strasbourg 12, rue du Général Zimmer, 67084 Strasbourg Cedex, France.
authors.forename: Anne-Catherine
authors.initials: AC
authors.lastname: Schmit
authors.affiliation: Institut de Biologie Moléculaire des Plantes, laboratoire propre du CNRS, (UPR 2357) conventionné avec l'Université de Strasbourg 12, rue du Général Zimmer, 67084 Strasbourg Cedex, France.
authors.forename: Guy
authors.initials: G
authors.lastname: Houlné
authors.affiliation: Institut de Biologie Moléculaire des Plantes, laboratoire propre du CNRS, (UPR 2357) conventionné avec l'Université de Strasbourg 12, rue du Général Zimmer, 67084 Strasbourg Cedex, France.
authors.forename: Marie-Edith
authors.initials: ME
authors.lastname: Chabouté
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D064007:Ataxia Telangiectasia Mutated Proteins; D001761:Bleomycin / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects; D003433:Crosses, Genetic; D053903:DNA Breaks, Double-Stranded / Q000187:drug effects; D004260:DNA Repair / Q000187:drug effects; D018744:DNA, Plant / Q000378:metabolism*; D050684:E2F Transcription Factors / Q000737:chemistry / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D035683:MicroRNAs / Q000378:metabolism; D010641:Phenotype; D021381:Protein Transport / Q000187:drug effects; D014026:Nicotiana / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: C576589:ATM protein, Arabidopsis; D029681:Arabidopsis Proteins; D018744:DNA, Plant; D050684:E2F Transcription Factors; D006657:Histones; D035683:MicroRNAs; D001761:Bleomycin; D049452:Green Fluorescent Proteins; D064007:Ataxia Telangiectasia Mutated Proteins