title: Specific DNA duplex formation at an artificial lipid bilayer: towards a new DNA biosensor technology.
abstract: A novel technique is described which comprises a base-specific DNA duplex formation at a lipid bilayer-H(2) O-phase boundary layer. Two different probes of oligonucleotides both carrying a double-tailed lipid at the 5'-terminus were incorporated into stable artificial lipid bilayers separating two compartments (cis/trans-channel) of an optically transparent microfluidic sample carrier with perfusion capabilities. Both the cis- and trans-channels are filled with saline buffer. Injection of a cyanine-5-labeled target DNA sequence, which is complementary to only one of the oligonucleotide probes, into the cis-channel, followed by a thorough perfusion, leads to an immobilization of the labeled complementary oligonucleotide on the membrane as detected by single-molecule fluorescence spectroscopy and microscopy. In the case of fluorescent but non-complementary DNA sequences, no immobilized fluorescent oligonucleotide duplex could be detected on the membrane. This clearly verifies a specific duplex formation at the membrane interface.
authors.affiliation: Organic Chemistry I - Bioorganic Chemistry, Institute of Chemistry, University of Osnabrück, Barbarastrasse 7, D-49069 Osnabrück.
authors.forename: Emma
authors.initials: E
authors.lastname: Werz
authors.forename: Sergei
authors.initials: S
authors.lastname: Korneev
authors.forename: Malayko
authors.initials: M
authors.lastname: Montilla-Martinez
authors.forename: Richard
authors.initials: R
authors.lastname: Wagner
authors.forename: Roland
authors.initials: R
authors.lastname: Hemmler
authors.forename: Claudius
authors.initials: C
authors.lastname: Walter
authors.forename: Jörg
authors.initials: J
authors.lastname: Eisfeld
authors.forename: Karsten
authors.initials: K
authors.lastname: Gall
authors.forename: Helmut
authors.initials: H
authors.lastname: Rosemeyer
mesh_terms: D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry*; D004261:DNA Replication; D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry*; D008055:Lipids; D009841:Oligonucleotides / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D014867:Water / Q000737:chemistry*
chemical_list: D008051:Lipid Bilayers; D008055:Lipids; D009841:Oligonucleotides; D014867:Water; D004247:DNA