title: Autoimmune dacryoadenitis and sialadenitis induced in rabbits by intravenous injection of autologous lymphocytes activated ex vivo against lacrimal antigens.
abstract: OBJECTIVE
  Autologous peripheral blood lymphocytes, activated in a mixed cell reaction when cocultured with purified rabbit lacrimal epithelial cells, are known to induce a severe autoimmune dacryoadenitis when injected directly into the donor animal's remaining inferior lacrimal gland (LG) or subcutaneously at a site remote from the LG. The purpose of the present study was to determine the ability of intravenously (IV) injected autologous stimulated lymphocytes to home to the LG and salivary gland (SG) and induce disease.
  
  
  METHODS
  One inferior LG was surgically excised from each rabbit. Acinar epithelial cells were purified, cultured for 2 days, gamma-irradiated, and cocultured for 5 days with purified autologous peripheral blood lymphocytes. The activated lymphocytes were used for autoadoptive transfer.
  
  
  RESULTS
  Tear production was reduced 50% by 4 weeks and tear breakup time was 70% less than normal. Ocular surface defects assessed by rose bengal staining were present but not as pronounced as after direct injection. Four weeks after IV injection, as after direct injection, glands contained large infiltrates composed of predominantly CD4(+) T cells close to interlobular and intralobular ducts; however, they also contained unique areas of streaming lymphocytes. Histopathology at 8 weeks was more severe than at 4 weeks, and SG also showed clusters of abnormal epithelial cells and streaming lymphocytes.
  
  
  CONCLUSIONS
  Lymphocytes activated against lacrimal antigens and injected IV can home to the LG and SG and initiate autoimmune processes, suggesting that these sites constitutively contain not only antigen-presenting cells displaying potentially pathogenic autoantigen epitopes but also chemokines and homing molecules that recruit CD4(+) T cells. This new rabbit model more closely mimics Sjögren syndrome, in that SG manifestations accompany the LG disease. It should be well suited to elucidating Sjögren pathogenesis and pathophysiology and to evaluating experimental therapies.
authors.affiliation: Ocular Surface Center, Doheny Eye Institute, Los Angeles, CA 90033, USA.
authors.forename: Rui Hua
authors.initials: RH
authors.lastname: Wei
authors.forename: Padmaja B
authors.initials: PB
authors.lastname: Thomas
authors.forename: Deedar M
authors.initials: DM
authors.lastname: Samant
authors.forename: Joel E
authors.initials: JE
authors.lastname: Schechter
authors.forename: Austin K
authors.initials: AK
authors.lastname: Mircheff
authors.forename: Melvin D
authors.initials: MD
authors.lastname: Trousdale
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D015703:Antigens, CD; D001324:Autoantigens / Q000276:immunology*; D001327:Autoimmune Diseases / Q000209:etiology* / Q000473:pathology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018920:Coculture Techniques; D003607:Dacryocystitis / Q000209:etiology* / Q000473:pathology; D015352:Dry Eye Syndromes / Q000209:etiology; D005260:Female; D007275:Injections, Intravenous; D007765:Lacrimal Apparatus / Q000276:immunology; D008213:Lymphocyte Activation / Q000502:physiology*; D011817:Rabbits; D012469:Salivary Glands / Q000276:immunology; D012793:Sialadenitis / Q000209:etiology* / Q000473:pathology; D013666:Tears / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D001324:Autoantigens