title: NMR structural analysis of the capsular polysaccharide from Streptococcus pneumoniae serotype 6C.
abstract: The original structure of Streptococcus pneumoniae capsular polysaccharide (CPS) serotype 6C was proposed based on chemical degradation and tandem mass analysis [J. Clin. Microbiol.2007, 45, 1225-1233]. In order to confirm the repeat unit structure and assign the stereochemical structure, the CPS 6C and the known CPS 6A were fully characterized by NMR spectroscopy. Full (1)H and (13)C NMR spectra assignments of CPS 6C and CPS 6A were achieved based on DQCOSY, TOCSY, HSQC, HMBC, and NOESY analysis. These analyses confirmed the published structure of CPS 6A and established the repeat unit structure of the CPS 6C as: →2)-α-D-Glcp-(1→3)-α-D-Glcp-(1→3)-α-L-Rhap-(1→3)-D-Ribitol-(5→phosphate-.
authors.affiliation: Pfizer Vaccine Research, 401 North Middletown Road, Pearl River, NY 10965, USA. ping.cai@pfizer.com
authors.forename: Ping
authors.initials: P
authors.lastname: Cai
authors.forename: Justin
authors.initials: J
authors.lastname: Moran
authors.forename: Viliam
authors.initials: V
authors.lastname: Pavliak
authors.forename: Chenghua
authors.initials: C
authors.lastname: Deng
authors.forename: Nancy
authors.initials: N
authors.lastname: Khoury
authors.forename: Olivier
authors.initials: O
authors.lastname: Marcq
authors.forename: Mark E
authors.initials: ME
authors.lastname: Ruppen
mesh_terms: D002214:Capsules; D002240:Carbohydrate Sequence; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000737:chemistry; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D011786:Quality Control; D013237:Stereoisomerism; D013296:Streptococcus pneumoniae / Q000737:chemistry*
chemical_list: D002214:Capsules; D009005:Monosaccharides; D011135:Polysaccharides, Bacterial