title: [Relevance between single nucleotide polymorphisms in nicotinic acetylcholine receptor genes and DNA damage levels in coke oven workers].
abstract: OBJECTIVE
  To investigate the relevance between single nucleotide polymorphisms (SNPs) in CHRNA3-CHRNB4-CHRNA5 gene cluster and DNA damage levels in the coke oven workers.
  
  
  METHODS
  A total of 309 male subjects from a steel plant in Northern China were divided into high exposure group (154 workers) and low exposure group (155 workers) according to the concentrations of plasma benzoapyrene-r-7, t-8, t-9, c-10-tetrahydrotetrol-albumin (BPDE-Alb) adducts. DNA damage levels of peripheral blood lymphocytes were examined by comet assay. Allelic discrimination of SNPs in CHRNA3-CHRNB4-CHRNA5 gene cluster were detected using Matrix-assisted laser desorption/ionization mass spectrometry.
  
  
  RESULTS
  The Olive tail moment (OTM) (1.23 ± 1.05) of peripheral blood lymphocytes in the high exposure group was significantly higher than that (0.80 ± 1.07) in the low exposure group (P < 0.01). In the high exposure group, OTM (1.64 ± 0.17) of subjects with rs667282CC genotype was significantly higher than those (0.95 ± 0.13 or 1.09 ± 0.11) of subjects with rs667282CT genotype or rs667282TT + CT genotype (P < 0.01); OTM (1.60 ± 0.17) of subjects with GG genotype at rs12910984 site was significantly higher than those (0.92 ± 0.13 or 1.07 ± 0.10) of subjects with AG genotype or AG + AA genotype (P < 0.01); OTM (1.35 ± 0.17 or 1.64 ± 0.17) of the TA/TA or CG/CG diplotype carriers was significantly higher than that (0.89 ± 0.13) of TA/CG diplotype carriers (P < 0.05). However, the relevance between genotypes of these SNPs and the DNA damage levels was not found in the low exposure group.
  
  
  CONCLUSIONS
  The rs667282CC genotype and rs12910984GG genotype on CHRNA3-CHRNB4-CHRNA5 gene cluster are associated with increased DNA damage levels in the high PAHs exposure group.
authors.affiliation: Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Yi-xiao
authors.initials: YX
authors.lastname: Tu
authors.forename: Huan
authors.initials: H
authors.lastname: Guo
authors.forename: Tang-chun
authors.initials: TC
authors.lastname: Wu
mesh_terms: D000328:Adult; D002681:China; D003077:Coke; D004249:DNA Damage; D005838:Genotype; D006801:Humans; D008297:Male; D005810:Multigene Family; D009419:Nerve Tissue Proteins / Q000235:genetics*; D016273:Occupational Exposure; D020641:Polymorphism, Single Nucleotide; D011978:Receptors, Nicotinic / Q000235:genetics*
chemical_list: D003077:Coke; D009419:Nerve Tissue Proteins; D011978:Receptors, Nicotinic; C447158:CHRNA5 protein, human