title: Regulation of xenobiotic transporter genes in liver and brain of juvenile thicklip grey mullets (Chelon labrosus) after exposure to Prestige-like fuel oil and to perfluorooctane sulfonate.
abstract: Xenobiotic transport proteins are involved in cellular defence against accumulation of xenobiotics participating in multixenobiotic resistance (MXR). In order to study the transcriptional regulation of MXR genes in fish exposed to common chemical pollutants we selected the thicklip grey mullet (Chelon labrosus), since mugilids are widespread in highly degraded estuarine environments where they have to survive through development and adulthood. Partial sequences belonging to genes coding for members of 3 different families of ATP binding cassette (ABC) transporter proteins (ABCB1; ABCB11; ABCC2; ABCC3; ABCG2) and a vault protein (major vault protein, MVP) were amplified and sequenced from mullet liver. Their liver and brain transcription levels were examined in juvenile mullets under exposure to perfluorooctane sulfonate (PFOS) and to fresh (F) and weathered (WF) Prestige-like heavy fuel oil for 2 and 16 days. In liver, PFOS significantly up-regulated transcription of abcb1, abcb11 and abcg2 while in brain only abcb11 was up-regulated. Both fuel treatments significantly down-regulated abcb11 in liver at day 2 while abcc2 was only down-regulated by WF. mvp was significantly up-regulated by F and down-regulated by WF at day 2 in the liver. At day 16 only a significant up-regulation of abcb1 in the F group was recorded. Brain abcc3 and abcg2 were down-regulated by both fuels at day 2, while abcb1 and abcc2 were only down-regulated by F exposure. After 16 days of exposure only abcb11 and abcg2 were regulated. In conclusion, exposure to organic xenobiotics significantly alters transcription levels of genes participating in xenobiotic efflux, especially after short periods of exposure. Efflux transporter gene transcription profiling could thus constitute a promising tool to assess exposure to common pollutants.
authors.affiliation: Dept. of Zoology and Animal Cell Biology, School of Science and Technology, University of the Basque Country (UPV/EHU), Leioa E-48940, Basque Country, Spain.
authors.forename: Oihane Diaz
authors.initials: OD
authors.lastname: de Cerio
authors.forename: Eider
authors.initials: E
authors.lastname: Bilbao
authors.forename: Miren P
authors.initials: MP
authors.lastname: Cajaraville
authors.forename: Ibon
authors.initials: I
authors.lastname: Cancio
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D017738:Alkanesulfonic Acids / Q000493:pharmacokinetics* / Q000633:toxicity*; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D029941:Fish Proteins / Q000235:genetics; D005466:Fluorocarbons / Q000493:pharmacokinetics* / Q000633:toxicity*; D005648:Fuel Oils / Q000633:toxicity*; D005786:Gene Expression Regulation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D023701:Smegmamorpha / Q000235:genetics* / Q000378:metabolism*; D020394:Vault Ribonucleoprotein Particles / Q000235:genetics; D014874:Water Pollutants, Chemical / Q000493:pharmacokinetics / Q000633:toxicity; D015262:Xenobiotics / Q000378:metabolism*; D000099187:Major Vault Protein
chemical_list: D018528:ATP-Binding Cassette Transporters; D017738:Alkanesulfonic Acids; D017931:DNA Primers; D029941:Fish Proteins; D005466:Fluorocarbons; D005648:Fuel Oils; D020394:Vault Ribonucleoprotein Particles; D014874:Water Pollutants, Chemical; D015262:Xenobiotics; D000099187:Major Vault Protein; C076994:perfluorooctane sulfonic acid