title: Differentiation of human osteoprogenitor cells increases after treatment with pulsed electromagnetic fields.
abstract: Human mesenchymal stem cells (hMSC) have become an important resource in developing strategies for regenerative medicine and tissue engineering, owing to their ability to renew and their potential for differentiation into cells of various types of tissues. Pulsed electromagnetic field (PEMF) stimulation has been used for several years in the treatment of fracture healing, with clinical beneficial effects, and several studies have demonstrated its capacity to increase bone tissue regeneration. In the present study, stromal cells of human bone marrow (BMSC), obtained from healthy donors, were appropriately expanded and underwent PEMF stimulation eight hours a day for fourteen days. Parameters such as proliferation and differentiation ability were evaluated on stimulated cultures. The evaluation of the marker expression was performed by RT-PCR for osteocalcin, by alkaline phosphatase quantitation and by histochemical stains. The results we obtained showed that BMSC treated with PEMF begin differentiation earlier than untreated BMSC, as shown by the markers used. The data show that PEMF is able to increase the osteogenic differentiation potential in adult mesenchymal cells isolated from young patients.
authors.affiliation: Department of Orthopedics, Traumatology, Rehabilitative and Plastic-Reconstructive Surgery, Second University of Naples, Naples, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Esposito
authors.forename: Angela
authors.initials: A
authors.lastname: Lucariello
authors.forename: Ilaria
authors.initials: I
authors.lastname: Riccio
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Riccio
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Esposito
authors.forename: Giovanni
authors.initials: G
authors.lastname: Riccardi
mesh_terms: D000328:Adult; D000469:Alkaline Phosphatase / Q000096:biosynthesis / Q000235:genetics; D001854:Bone Marrow Cells / Q000166:cytology / Q000528:radiation effects; D002113:Calcification, Physiologic / Q000528:radiation effects; D002453:Cell Cycle / Q000528:radiation effects; D002478:Cells, Cultured / Q000166:cytology / Q000528:radiation effects; D003470:Culture Media / Q000494:pharmacology; D004574:Electromagnetic Fields; D004790:Enzyme Induction / Q000528:radiation effects; D018507:Gene Expression Regulation, Developmental / Q000528:radiation effects; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000528:radiation effects*; D015675:Osteocalcin / Q000096:biosynthesis / Q000235:genetics; D010011:Osteocytes / Q000166:cytology*; D010012:Osteogenesis / Q000187:drug effects / Q000528:radiation effects*; D017154:Stromal Cells / Q000166:cytology / Q000528:radiation effects
chemical_list: D003470:Culture Media; D015675:Osteocalcin; D000469:Alkaline Phosphatase