title: Agitation and aeration effects in suspension mammalian cell cultures.
abstract: Three different hybridoma cell lines, grown in serum-free media with different levels of Pluronic F-68, were subjected to a shear force of 0.6 N m(-2). Some protective effect due to the polymer was found, indicating it to be a potentially useful adjuvant in serum-free media. Other observations of liquid and gas effects at the reactor level have been included here. A discussion of the difference between suspension and microcarrier cultures, in relation to hydrodynamic effects, is included.
authors.affiliation: Dept. of Infectious Diseases, The University of Sydney, 2006, NSW, Australia.
authors.forename: C P
authors.initials: CP
authors.lastname: Marquis
authors.forename: K S
authors.initials: KS
authors.lastname: Low
authors.forename: J P
authors.initials: JP
authors.lastname: Barford
authors.forename: C
authors.initials: C
authors.lastname: Harbour