title: A preliminary study on the FAM5C expression in  generalized chronic periodontitis.
abstract: OBJECTIVE
  The Family with sequence similarity 5 member C (FAM5C) has been suggested to contribute in aggressive periodontitis. However, there is no data regarding its role in chronic periodontitis. The aim of this study was to evaluate the FAM5C expression in chronic periodontitis and to study association of FAM5C with key immunoinflammatory markers.
  
  
  METHODS
  Gingival biopsies were harvested from periodontally healthy subjects (n = 10) and chronic periodontitis subjects (n = 15). The levels of mRNA of FAM5C, interleukin (IL)-17, IL-6, IL-23, IL-10, IL-4, interferon-c, toll-like receptor (TLR)-2, TLR-4, osteoprotegerin (OPG), receptor activator of NF-κB ligand (RANKL), tumor necrosis factor (TNF)-a, transforming growth factor-b, transcription factor forkhead box p3, and transcription factor orphan nuclear receptor C2 were evaluated by real-time polymerase chain reaction.
  
  
  RESULTS
  FAM5C mRNA levels were not different between periodontally healthy and diseased tissues (P > 0.05). Gene expressions of IL-17, TNF-a, OPG, RANKL, TLR-2, and TLR-4 were higher in periodontitis, when compared to periodontally healthy sites (P < 0.05), while no differences between groups were observed for the other genes evaluated (P > 0.05). There were no correlations between the gene expression of FAM5C and the other immunoinflammatory markers (P > 0.05).
  
  
  CONCLUSIONS
  Within the limits of this study, it seems that FAM5C expression does not contribute to chronic periodontitis.
authors.affiliation: Dental Research Division, School of Dentistry, Paulista University, São Paulo, Brazil.
authors.forename: F V
authors.initials: FV
authors.lastname: Ribeiro
authors.forename: V R
authors.initials: VR
authors.lastname: Santos
authors.forename: M F
authors.initials: MF
authors.lastname: Bastos
authors.forename: T S
authors.initials: TS
authors.lastname: De Miranda
authors.forename: A R
authors.initials: AR
authors.lastname: Vieira
authors.forename: L C
authors.initials: LC
authors.lastname: De Figueiredo
authors.forename: P M
authors.initials: PM
authors.lastname: Duarte
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D055113:Chronic Periodontitis / Q000235:genetics* / Q000378:metabolism*; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D005881:Gingiva / Q000473:pathology; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism*; D007378:Interleukins / Q000096:biosynthesis / Q000235:genetics; D008297:Male; D008875:Middle Aged; D024101:Mitochondrial Proteins / Q000096:biosynthesis / Q000235:genetics*; D010512:Periodontal Index; D010865:Pilot Projects; D053245:RANK Ligand / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D018709:Statistics, Nonparametric; D051195:Toll-Like Receptor 2 / Q000096:biosynthesis / Q000235:genetics; D051197:Toll-Like Receptor 4 / Q000096:biosynthesis / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics
chemical_list: C528897:BRINP3 protein, human; D016207:Cytokines; D004268:DNA-Binding Proteins; D018836:Inflammation Mediators; D007378:Interleukins; D024101:Mitochondrial Proteins; D053245:RANK Ligand; D012333:RNA, Messenger; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D014409:Tumor Necrosis Factor-alpha