title: DNA replication fading as proliferating cells advance in their commitment to terminal differentiation.
abstract: Terminal differentiation is the process by which cycling cells stop proliferating to start new specific functions. It involves dramatic changes in chromatin organization as well as gene expression. In the present report we used cell flow cytometry and genome wide DNA combing to investigate DNA replication during murine erythroleukemia-induced terminal cell differentiation. The results obtained indicated that the rate of replication fork movement slows down and the inter-origin distance becomes shorter during the precommitment and commitment periods before cells stop proliferating and accumulate in G1. We propose this is a general feature caused by the progressive heterochromatinization that characterizes terminal cell differentiation.
authors.affiliation: Department of Cell Proliferation & Development, Centro de Investigaciones Biológicas (CSIC), Ramiro de Maeztu 9, Madrid, Spain.
authors.forename: Monturus Ma
authors.initials: MM
authors.lastname: Estefanía
authors.forename: Olivier
authors.initials: O
authors.lastname: Ganier
authors.forename: Pablo
authors.initials: P
authors.lastname: Hernández
authors.forename: Jorge B
authors.initials: JB
authors.lastname: Schvartzman
authors.forename: Marcel
authors.initials: M
authors.lastname: Mechali
authors.forename: Dora B
authors.initials: DB
authors.lastname: Krimer
mesh_terms: D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D017931:DNA Primers; D004261:DNA Replication; D005434:Flow Cytometry; D019084:Fluorescent Antibody Technique, Indirect; D051379:Mice
chemical_list: D017931:DNA Primers