title: Establishment of a tractable genetic transformation system in Veillonella spp.
abstract: We have constructed the first Escherichia coli-Veillonella shuttle vector based on an endogenous plasmid (pVJL1) isolated from a clinical Veillonella strain. A highly transformable Veillonella strain was also identified. Both the shuttle vector and the transformable strain should be valuable tools for future Veillonella genetic studies.
authors.affiliation: Department of Microbiology and Immunology, College of Medicine, College of Dentistry, University of Oklahoma Health SciencesCenter, Oklahoma City, Oklahoma, USA.
authors.forename: Jinman
authors.initials: J
authors.lastname: Liu
authors.forename: Zhoujie
authors.initials: Z
authors.lastname: Xie
authors.forename: Justin
authors.initials: J
authors.lastname: Merritt
authors.forename: Fengxia
authors.initials: F
authors.lastname: Qi
mesh_terms: D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D005827:Genetics, Microbial / Q000379:methods*; D008967:Molecular Biology / Q000379:methods*; D008969:Molecular Sequence Data; D010957:Plasmids; D017422:Sequence Analysis, DNA; D014170:Transformation, Genetic; D014678:Veillonella / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial