title: [Generation of rat induced pluripotent stem cells: the analysis of reprogramming and culturing media].
abstract: The rat represents very important, superior in many respects to the mous, animal model for studying pharmacology, physiology, ageing, cardiovascular etc. However, numerous attempts to derive rat ES cells necessary to carry out loss-of-gene-function studies have not been successful thus far. Therefore rat induct pluripotent stem cells (or riPS) should provide a notable alternative to ES cell, allowing to study gene functions in this valuable animal model. Here we report an improved lentivirus-based riPS derivation protocol that makes use of small inhibitors of MEK and GSK3. We show that the excision of proviruses does not affect neither karyotype and pluripotency state of these cells. Also, we propose genetic tool for an improvement of the quality of riPS cells in culture. These data may prompt further iPS-based gene targeting in rat as well as the development iPS-based gene therapies, using this animal model.
authors.forename: M A
authors.initials: MA
authors.lastname: Liskovykh
authors.forename: I A
authors.initials: IA
authors.lastname: Chuĭkin
authors.forename: A
authors.initials: A
authors.lastname: Ranjan
authors.forename: D A
authors.initials: DA
authors.lastname: Safina
authors.forename: E N
authors.initials: EN
authors.lastname: Tolkunova
authors.forename: Iu M
authors.initials: IuM
authors.lastname: Minina
authors.forename: N S
authors.initials: NS
authors.lastname: Zhdanova
authors.forename: P A
authors.initials: PA
authors.lastname: Dyban
authors.forename: J
authors.initials: J
authors.lastname: Mullins
authors.forename: E I
authors.initials: EI
authors.lastname: Kostyleva
authors.forename: E V
authors.initials: EV
authors.lastname: Chikhirzhina
authors.forename: M
authors.initials: M
authors.lastname: Bader
authors.forename: N
authors.initials: N
authors.lastname: Alenina
authors.forename: A N
authors.initials: AN
authors.lastname: Tomilin
mesh_terms: D000818:Animals; D054337:Cell Dedifferentiation / Q000502:physiology*; D002460:Cell Line; D003470:Culture Media; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D016086:Lentivirus; D051379:Mice; D051381:Rats; D014161:Transduction, Genetic / Q000379:methods
chemical_list: D003470:Culture Media