title: Evaluation of 14-3-3 protein family levels and associated receptor expression of estrogen and progesterone in human uterine leiomyomas.
abstract: OBJECTIVE
  Uterine leiomyomas represents a major public health problem. Despite their prevalence, the causation and pathogenesis of leiomyomas are poorly understood. A broad range of organisms and tissues contain 14-3-3 proteins which have been associated with the pathogenesis of many diseases through participating in signal transduction pathways. This study was designed to evaluate which 14-3-3 isoforms might be optimal targets in leiomyomas, and to further explore their relationship with estrogen and progesterone receptor (ER and PR).
  
  
  METHODS
  Paired samples of leiomyoma and adjacent myometrium were obtained from 80 subjects who had surgical excision of uterine leiomyomas. The expression of 14-3-3 isoforms was detected by Western bolt and RT-PCR, and their relationship with ER and PR was analysed by immunohistochemistry.
  
  
  RESULTS
  The expressions of 14-3-3σ had decreased significantly in leiomyoma compared with that in normal myometrium and was negatively correlated with ER and PR by immunohistochemistry.
  
  
  CONCLUSIONS
  The down-regulation of 14-3-3σ in leiomyoma suggests that 14-3-3σ may play a role in tumorigenesis, and that its mechanism may be involved in the up-regulation of ER and PR.
authors.affiliation: Division of Obstetrics, Jiangxi Maternal & Child Health Hospital, Nanchang, PR China.
authors.forename: Liqun
authors.initials: L
authors.lastname: Wang
authors.forename: Huang
authors.initials: H
authors.lastname: Huang
authors.forename: Dan
authors.initials: D
authors.lastname: Liu
authors.forename: Shufeng
authors.initials: S
authors.lastname: Fang
authors.forename: Yan
authors.initials: Y
authors.lastname: Xian
authors.forename: Jiangyan
authors.initials: J
authors.lastname: Zhou
authors.forename: Yang
authors.initials: Y
authors.lastname: Zuo
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Ouping
authors.initials: O
authors.lastname: Huang
authors.forename: Ming
authors.initials: M
authors.lastname: He
mesh_terms: D048948:14-3-3 Proteins / Q000235:genetics / Q000378:metabolism*; D000328:Adult; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D015536:Down-Regulation; D005092:Exonucleases / Q000235:genetics / Q000378:metabolism*; D005095:Exoribonucleases; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D007889:Leiomyoma / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D008875:Middle Aged; D009215:Myometrium / Q000378:metabolism / Q000473:pathology; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D011960:Receptors, Estrogen / Q000378:metabolism*; D011980:Receptors, Progesterone / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation; D014594:Uterine Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000601:surgery
chemical_list: D048948:14-3-3 Proteins; D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D005092:Exonucleases; D005095:Exoribonucleases; C413442:SFN protein, human