title: Immunochemical applications of avidin-biotin technology.
abstract: The major advantage in the use of avidin-biotin technology for isolation purposes is that an improved capacity for purification of an antigen often results (1,2). The antibody, bound to the column via an avidin-biotin bridge, is less affected by the chemistry of immobilization or by physical interactions (hydrophobic, electrostatic, salting-out effects, precipitation, and so on) with the solid support. Such potential deleterious effects are borne by avidin, which is a highly stable protein, and thus serves as a physicochemical buffer for the antibody.
authors.affiliation: Department of Biophysics, The Weizmann Institute of Science, Rehovot, Israel.
authors.forename: E A
authors.initials: EA
authors.lastname: Bayer
authors.forename: M
authors.initials: M
authors.lastname: Wilchek