title: In vitro antibacterial and time-kill assessment of crude methanolic stem bark extract of Acacia mearnsii de wild against bacteria in shigellosis.
abstract: Shigellosis is an important cause of worldwide morbidity and mortality among young children and old people for which treatment with antimicrobial agents is limited. Hence, the need for curative potentials obtainable from medicinal plants becomes inevitable. This study was carried out to assess the antibacterial potentials of crude methanolic extract of the stem bark of Acacia mearnsii against some selected bacteria of clinical importance in shigellosis. The bacteria were inhibited by the extract to produce concentration dependent inhibition zones. The extract exhibited a varied degree of antibacterial activity against all the tested isolates. The MIC values for Gram negative (0.0391-0.3125) mg/mL and those of Gram positive bacteria (0.0781-0.625) mg/mL indicated that the Gram negative bacteria were more inhibited by the extract than the Gram positive bacteria. Average log reduction in viable cell count in time-kill assay ranged between -2.456 Log₁₀ to 2.230 Log₁₀ cfu/mL after 4 h of interaction, and between -2.921 Log₁₀ and 1.447 Log₁₀ cfu/mL after 8 h interaction in 1× MIC and 2× MIC of the extract. The study provided scientific justification for the use of the crude methanolic extract from the stem bark of A. mearnsii in shigellosis. The degree of the antibacterial activity indicated that the crude extract is a potential source of bioactive compounds that could be useful for the development of new antimicrobial agents capable of decreasing the burden of drug resistance and cost of management of diseases of clinical and public health importance in South Africa.
authors.affiliation: Phytomedicine Research Centre, Department of Botany, University of Fort Hare, Alice 5700, South Africa.
authors.forename: Olufunmiso Olusola
authors.initials: OO
authors.lastname: Olajuyigbe
authors.forename: Anthony Jide
authors.initials: AJ
authors.lastname: Afolayan
mesh_terms: D000045:Acacia / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D045424:Complex Mixtures / Q000737:chemistry* / Q000494:pharmacology*; D004405:Dysentery, Bacillary / Q000188:drug therapy; D000432:Methanol / Q000737:chemistry; D008826:Microbial Sensitivity Tests; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D011634:Public Health; D013019:South Africa
chemical_list: D000900:Anti-Bacterial Agents; D045424:Complex Mixtures; D010936:Plant Extracts; D000432:Methanol