title: [Growth of osteoblasts following culture on two collagen scaffolds].
abstract: OBJECTIVE
  To explore the growth of osteoblasts following culture on two absorbable collagen scaffolds.
  
  
  METHODS
  MC3T3-E1 osteoblast cell line was inoculated on two collagen scaffolds (BME-10X and Bio-Gide) and co-cultured in vitro. The adhesion and migration of cells were detected by optical microscope. Cell proliferation was detected using CCK-8 reagent kit. The ultrastructure of the adhesion between cells and scaffolds were observed using electronic microscopy.
  
  
  RESULTS
  MC3T3-E1 osteoblast cell line could adhere, migrate, and proliferate on both two membranes. The proliferation of cells showed no significant difference between porous layer and nonporous layer of BME-10X (P > 0.05), while the rate of proliferation was significantly different between loose layer and dense layer of Bio-Gide (P < 0.05).
  
  
  CONCLUSIONS
  Both BME-10X and Bio-Gide have good biocompatibility with MC3T3-E1 osteoblast cell line, and the double-layer structure of Bio-Gide can prevent the cells to grow into the dense layer.
authors.affiliation: Department of Stomatology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Ma
authors.forename: Ding
authors.initials: D
authors.lastname: Zhang
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Xiao-Jiao
authors.initials: XJ
authors.lastname: Li
mesh_terms: D001672:Biocompatible Materials; D002448:Cell Adhesion; D002460:Cell Line; D002465:Cell Movement; D049109:Cell Proliferation; D003094:Collagen; D006801:Humans; D010006:Osteoblasts / Q000166:cytology* / Q000648:ultrastructure; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D003094:Collagen