title: [Effect of extracts from Radix Trichosanthis on the expression of HBsAg and HBeAg in HepG2.2.15 cells].
abstract: OBJECTIVE
  To investigate the effect of extracts with water and alcohol from Radix Trichosanthis on the cell survival and the expression of hepatitis B surface antigen (HBsAg) and hepatitis B e-antigen (HBeAg) in HepG2.2.15 cell supernatant.
  
  
  METHODS
  The cell survival rate of HepG2.2.15 cells was detected by MTT assay. The HBsAg and HBeAg in HepG 2.2.15 cell supernatant were evaluated by enzyme linked immunosorbent assay.
  
  
  RESULTS
  The water and alcohol soluble extracts from Radix Trichosanthis significantly inhibited the levels of HBsAg and HBeAg in HepG2.2.15 cells in a time-and-concentration-dependent manner. However, the therapeutic index of extracts with water from Radix Trichosanthis was better than that in the alcohol group.
  
  
  CONCLUSIONS
  The activity of water-soluble extract from Radix Trichosanthis is stronger on anti-hepatitis B virus than that of the alcohol-soluble extract.
authors.affiliation: Central South University, Changsha, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Chen
authors.forename: Zhaofa
authors.initials: Z
authors.lastname: Xu
authors.forename: Hongtao
authors.initials: H
authors.lastname: Ouyang
authors.forename: Minyuan
authors.initials: M
authors.lastname: Peng
authors.forename: Ting
authors.initials: T
authors.lastname: Wu
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Yanping
authors.initials: Y
authors.lastname: Liu
authors.forename: Huiwen
authors.initials: H
authors.lastname: Yan
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000145:classification / Q000494:pharmacology*; D056945:Hep G2 Cells; D006514:Hepatitis B Surface Antigens / Q000096:biosynthesis*; D006513:Hepatitis B e Antigens / Q000096:biosynthesis*; D006515:Hepatitis B virus / Q000187:drug effects; D006801:Humans
chemical_list: D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens