title: [Construction of tissue engineering bone with bone marrow stromal cell sheets].
abstract: OBJECTIVE
  To construct tissue engineering bone with bone marrow stromal cell (BMSC) sheets of dogs.
  
  
  METHODS
  BMSC were derived from dog bone marrow and cell sheets were prepared with temperature-responsive dishes after the cells were induced by osteogenesis. Allogeneic dogs decalcification bone matrixes (DBM) were prepared. Sixteen dogs were divided into 4 groups. The MSC cell sheets-rhBMP-2-BMSC-DBM were implanted under the left latissimus dorsi myofascial as the experimental side; while the rhBMP-2-BMSC-DBM were implanted in the right side as the control. Ectopic bone formation in vivo was evaluated by histological examination 4, 8, 12, 16 weeks after operation.
  
  
  RESULTS
  The osteogenesis in the experimental group was better than that in the control group. New bone area in the experimental side was larger than that in the control group, and the difference was significant (P < 0.05). After 16 weeks, lamellar bone was connected into a film in the experimental group. Haversian system and red bone marrow could be seen.
  
  
  CONCLUSIONS
  BMSC cell sheets could promote the bone formation of functional tissue-engineered bone.
authors.affiliation: Department of Oral and Maxillofacial Surgery, The Affiliated Hospital of Medical College, Qingdao University, Qingdao Shandong 266003, China.
authors.forename: Ling-xue
authors.initials: LX
authors.lastname: Bu
authors.forename: Yan-hui
authors.initials: YH
authors.lastname: Wang
authors.forename: Ning-yi
authors.initials: NY
authors.lastname: Li
authors.forename: Zheng-hua
authors.initials: ZH
authors.lastname: Gao
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.forename: Heng
authors.initials: H
authors.lastname: Jing
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells; D001857:Bone Matrix; D001842:Bone and Bones; D004285:Dogs; D059630:Mesenchymal Stem Cells; D017154:Stromal Cells; D023822:Tissue Engineering