title: Gene Diversity of Trichomonas vaginalis Isolates.
abstract: BACKGROUND
  Trichomonas vaginalis is protozoan parasite responsible for trichomoniasis and is more common in high-risk behavior group such as prostitute individuals. Interest in trichomoniasis is due to increase one's susceptibility to viruses such as herpes, human papillomavirus and HIV. The aim of this study was to find genotypic differences between the isolates.
  
  
  METHODS
  Forty isolates from prisoners' women in Tehran province were used in this study. The random amplified polymorphic DNA (RAPD) technique was used to determine genetic differences among isolates and was correlated with patient's records. By each primer the banding pattern size of each isolates was scored (bp), genetic differences were studied, and the genealogical tree was constructed by using NTSYS software program and UPGMA method.
  
  
  RESULTS
  The least number of bands were seen by using primer OPD8 and the most by using OPD3. Results showed no significant difference in isolates from different geographical areas in Iran. By using primer OPD1 specific amplified fragment with length 1300 base pair were found in only 8 isolates. All these isolates were belonged to addicted women; however, six belonged to asymptomatic patients and two to symptomatic ones.
  
  
  CONCLUSIONS
  There was not much genetic diversity in T vaginalis isolates from three different geographical areas.
authors.affiliation: Dept. of Parasitology, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Z
authors.initials: Z
authors.lastname: Valadkhani
authors.forename: F
authors.initials: F
authors.lastname: Kazemi
authors.forename: N
authors.initials: N
authors.lastname: Hassan
authors.forename: Z
authors.initials: Z
authors.lastname: Aghighi
authors.forename: I
authors.initials: I
authors.lastname: Esmaili
authors.forename: M
authors.initials: M
authors.lastname: Talebi