title: TsPAP1 encodes a novel plant prolyl aminopeptidase whose expression is induced in response to suboptimal growth conditions.
abstract: A triticale cDNA encoding a prolyl aminopeptidase (PAP) was obtained by RT-PCR and has been designated as TsPAP1. The cloned cDNA is 1387 bp long and encodes a protein of 390 amino acids with a calculated molecular mass of 43.9 kDa. The deduced TsPAP1 protein exhibits a considerable sequence identity with the biochemically characterized bacterial and fungal PAP proteins of small molecular masses (∼35 kDa). Moreover, the presence of conserved regions that are characteristic for bacterial monomeric PAP enzymes (the GGSWG motif, the localization of the catalytic triad residues and the segment involved in substrate binding) has also been noted. Primary structure analysis and phylogenetic analysis revealed that TsPAP1 encodes a novel plant PAP protein that is distinct from the multimeric proteins that have thus far been characterized in plants and whose counterparts have been recognized only in bacteria and fungi. A significant increase in the TsPAP1 transcript level in the shoots of triticale plants was observed under drought and saline conditions as well as in the presence of cadmium and aluminium ions in the nutrient medium. This paper is the first report describing changes in the transcript levels of any plant PAP in response to suboptimal growth conditions.
authors.affiliation: Department of Biochemistry, Warsaw University of Life Sciences - SGGW, Nowoursynowska 159, 02-776 Warsaw, Poland.
authors.forename: Urszula
authors.initials: U
authors.lastname: Szawłowska
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Grabowska
authors.forename: Edyta
authors.initials: E
authors.lastname: Zdunek-Zastocka
authors.forename: Wiesław
authors.initials: W
authors.lastname: Bielawski
mesh_terms: D000595:Amino Acid Sequence; D000626:Aminopeptidases / Q000096:biosynthesis* / Q000145:classification / Q000235:genetics; D002523:Edible Grain / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000096:biosynthesis* / Q000145:classification / Q000235:genetics; D013312:Stress, Physiological / Q000235:genetics
chemical_list: D010940:Plant Proteins; D000626:Aminopeptidases; C022450:prolyl aminopeptidase