title: Effect of expansion media containing fibroblast growth factor-2 and dexamethasone on the chondrogenic potential of human adipose-derived stromal cells.
abstract: hASCs [human ASCs (adipose derived stromal cells)] proliferate more rapidly in the presence of basic FGF-2 (fibroblast growth factor-2) and Dex (dexamethasone). We have examined the effects of expanding hASCs in media containing these two factors on their chondrogenic differentiation potential. Results show that the addition of FGF-2 and Dex to the expansion medium does not remarkably alter the chondrogenic potential of the cells induced by BMP-6 (bone morphogenetic protein-6), based on chondrogenic gene expression, sGAG (sulfated glycosaminoglycan) accumulation and immunohistochemical observation. This is in direct contrast to previously reported promotion of the osteogenic and adipogenic potential of hASCs by these two factors. Therefore, an expansion medium containing FGF-2, with or without Dex, is appropriate for the fast expansion of hASCs without compromising chondrogenic potential.
authors.affiliation: Graduate Bioengineering Program, The University of Notre Dame, Notre Dame, IN 46556, USA.
authors.forename: Yongxing
authors.initials: Y
authors.lastname: Liu
authors.forename: Diane R
authors.initials: DR
authors.lastname: Wagner
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D055418:Bone Morphogenetic Protein 6 / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D020219:Chondrogenesis / Q000187:drug effects*; D003470:Culture Media; D003907:Dexamethasone / Q000494:pharmacology*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D006025:Glycosaminoglycans / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D017154:Stromal Cells / Q000166:cytology*
chemical_list: D055418:Bone Morphogenetic Protein 6; D003470:Culture Media; D006025:Glycosaminoglycans; D016222:Fibroblast Growth Factor 2; C013786:A73025; D003907:Dexamethasone