title: A nanostructured piezoelectric immunosensor for detection of human cardiac troponin T.
abstract: A piezoelectric immunosensor based on gold nanoparticles (AuNPs) co-immobilized on a dithiol-modified surface is proposed for detection of human cardiac troponin T (TnT). Anti-human troponin T (anti-TnT) antibodies were covalently immobilized on the nanostructured electrode surface by thiol-aldehyde linkages. In a homogeneous bulk solution, TnT was captured by anti-TnT immobilized on the QCM electrode. Cyclic voltammetry studies were used to characterize the AuNPs layer on the electrode surface and the anti-TnT immobilization steps. The QCM-flow immunosensor exhibited good reliability, measuring concentrations of TnT from 0.003 to 0.5 ng mL(-1) in human serum with high linearity (r = 0.989; p < 0.01). The immunosensor exhibited a 7% coefficient of variation and 0.0015 ng mL(-1) limit of detection, indicating a high reproducibility and sensitivity. The proposed QCM nanostructured immunosensor is easy to use and has promising potential in the diagnosis of acute myocardial infarction due to its speed and high sensitivity.
authors.affiliation: Laboratório de Engenharia Biomédica, Universidade Federal de Pernambuco, Av. Prof. Moraes Rego, 1235, Recife, Pernambuco 50670-901, Brazil. rosanafonseca@hotmail.com
authors.forename: Rosana A S
authors.initials: RA
authors.lastname: Fonseca
authors.forename: Joilson
authors.initials: J
authors.lastname: Ramos-Jesus
authors.forename: Lauro T
authors.initials: LT
authors.lastname: Kubota
authors.forename: Rosa F
authors.initials: RF
authors.lastname: Dutra
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology; D015374:Biosensing Techniques / Q000379:methods*; D003538:Cystamine / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D005998:Glycine / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007118:Immunoassay / Q000379:methods; D009203:Myocardial Infarction / Q000175:diagnosis; D053758:Nanoparticles / Q000737:chemistry*; D009994:Osmolar Concentration; D010084:Oxidation-Reduction; D012680:Sensitivity and Specificity; D044967:Serum / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry; D020107:Troponin T / Q000032:analysis* / Q000097:blood / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D013438:Sulfhydryl Compounds; D020107:Troponin T; D006046:Gold; D003538:Cystamine; C501433:1,6-hexanedithiol; D005998:Glycine