title: Expression of VEGF-receptors in TMJ synovium of rabbits with experimentally induced internal derangement.
abstract: Our aim was to evaluate the expression of vascular endothelial growth factor receptors (VEGFRs) in the synovium of the temporomandibular joints (TMJ) of rabbits with experimentally induced internal derangement. Internal derangement was experimentally induced in 52 rabbit TMJ, and established on the right side of TMJ while the left side was used as the control. Each joint and its control was evaluated by magnetic resonance imaging (MRI) and endoscopy. The synovial tissues on both sides were harvested after one, two, three, and four weeks. The expression of VEGFRs mRNA was investigated in the experimental joint and its control using real-time polymerase chain reaction (PCR). Internal derangement was successfully confirmed in 45 of the 52 of the experimental joints (87%) on the right side by MRI and endoscopy. In the first and fourth week, the VEGFR-2 mRNA expression was higher in the experimental joints than in the controls (P=0.008 and P=0.02). Meanwhile, the VEGFR-1 mRNA expression was up-regulated in the experimental group compared with the controls during the fourth week (P=0.02). However, we found no significant differences in VEGFR-3 mRNA expression in the two groups during the first and fourth weeks. During the second and third weeks, the mRNA expression of the three receptors did not differ significantly among the groups. Our data have shown increased expression of VEGFR-1 and VEGFR-2 mRNA in the synovium of rabbit TMJ with internal derangement, which indicates that VEGFR-1 and VEGFR-2 may have important roles in the processes of internal derangement and formation of adhesions.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Ninth People's Hospital, Collage of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
authors.forename: ShanYong
authors.initials: S
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Cao
authors.forename: KuiJie
authors.initials: K
authors.lastname: Wei
authors.forename: XiuMing
authors.initials: X
authors.lastname: Liu
authors.forename: YuanJin
authors.initials: Y
authors.lastname: Xu
authors.forename: Chi
authors.initials: C
authors.lastname: Yang
authors.forename: Gerhard
authors.initials: G
authors.lastname: Undt
authors.forename: Majd S
authors.initials: MS
authors.lastname: Haddad
authors.forename: WanTao
authors.initials: W
authors.lastname: Chen
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004204:Joint Dislocations / Q000378:metabolism / Q000473:pathology; D008279:Magnetic Resonance Imaging; D012333:RNA, Messenger; D011817:Rabbits; D060888:Real-Time Polymerase Chain Reaction; D040262:Receptors, Vascular Endothelial Growth Factor / Q000378:metabolism*; D013583:Synovial Membrane / Q000378:metabolism* / Q000473:pathology; D013704:Temporomandibular Joint / Q000378:metabolism* / Q000473:pathology; D013705:Temporomandibular Joint Disorders / Q000378:metabolism* / Q000473:pathology; D000267:Tissue Adhesions / Q000209:etiology* / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D040262:Receptors, Vascular Endothelial Growth Factor