title: Effects of sterigmatocystin on TNF-α, IL-6 and IL-12 expression in murine             peripheral blood mononuclear cells and peritoneal macrophages in vivo.
abstract: Sterigmatocystin (ST) is a toxic metabolite mainly produced by the fungi             Aspergillus nidulans and Aspergillus versicolor. ST is considered a potent carcinogen,             mutagen and teratogen. However, over the past few years, it has been demonstrated             that it is less acutely toxic to rodents in vivo. In this study, we evaluated             the putative effects of ST on the expression of tumor necrosis factor-α (TNF-α),             interleukin (IL)-6 and IL-12 at mRNA levels in murine peripheral blood mononuclear             cells (mPBMCs) and peritoneal macrophage cells and on the serum TNF-α and IL-6             levels in BALB/c mice. Our results show the downregulation of TNF-α, IL-6 and             IL-12 mRNA expression in mPBMCs and peritoneal macrophage cells using semi-quantitative             reverse transcription-polymerase chain reaction following ST treatment by intraperitoneal             injection. Additionally, serum TNF-α and IL-6 levels were also decreased as shown             by enzyme-linked immunosorbent assay (ELISA). These results suggest that ST contamination             has negative immunomodulatory effects through the downregulation of cytokine expression             and secretion.
authors.affiliation: Department of Pathology, Second Hospital, Hebei Medical University, Shijiazhuang, Hebei 050017, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zhi Gang
authors.initials: ZG
authors.lastname: Yao
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.forename: Ling Xiao
authors.initials: LX
authors.lastname: Xing
authors.forename: Yan
authors.initials: Y
authors.lastname: Xia
authors.forename: Xiang Hong
authors.initials: XH
authors.lastname: Zhang
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation / Q000187:drug effects*; D007155:Immunologic Factors / Q000494:pharmacology*; D018664:Interleukin-12 / Q000096:biosynthesis*; D015850:Interleukin-6 / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013241:Sterigmatocystin / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
chemical_list: D007155:Immunologic Factors; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D013241:Sterigmatocystin; D018664:Interleukin-12