title: Reactive oxygen species regulate the replication of porcine circovirus type 2 via NF-κB pathway.
abstract: Intracellular redox state has been suggested to have various effects on the replication of different viruses within host cells. The aim of the present study was to investigate the influence of reactive oxygen species (ROS) on replication of porcine circovirus type 2 (PCV2), in PK15 cells. Following PCV2 infection there was a time-dependent increase in ROS. Antioxidant N-acetyl-l-cysteine treatment of cells resulted in lower ROS levels and lower PCV2 replication. In contrast, treatment by buthionine sulfoximine (BSO), a GSH synthesis inhibitor, resulted in elevation of ROS levels and increased PCV2 replication. Furthermore, inhibiting the activity of NF-κB, a redox-responsive transcription factor, suppressed BSO-mediated increase of PCV2 replication, indicating that increased PCV2 replication likely occurs via ROS activation of NF-κB. Taken together, our results indicate that the generation of ROS during PCV2 infection is involved in its replication and this progression is associated with the alteration in NF-κB activity induced by ROS.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Xingxiang
authors.initials: X
authors.lastname: Chen
authors.forename: Fei
authors.initials: F
authors.lastname: Ren
authors.forename: John
authors.initials: J
authors.lastname: Hesketh
authors.forename: Xiuli
authors.initials: X
authors.lastname: Shi
authors.forename: Junxian
authors.initials: J
authors.lastname: Li
authors.forename: Fang
authors.initials: F
authors.lastname: Gan
authors.forename: Kehe
authors.initials: K
authors.lastname: Huang
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D019328:Buthionine Sulfoximine / Q000494:pharmacology; D018173:Circoviridae Infections / Q000378:metabolism / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013552:Swine; D013553:Swine Diseases / Q000378:metabolism* / Q000821:virology; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000975:Antioxidants; D016328:NF-kappa B; D017382:Reactive Oxygen Species; D019328:Buthionine Sulfoximine; D000111:Acetylcysteine