title: Biochemical and physiological determinants of intramolecular isotope patterns in sucrose from C₃, C₄ and CAM plants accessed by isotopic ¹³C NMR spectrometry: a viewpoint.
abstract: This paper discusses the biochemical and physiological factors underlying the site-specific, non-random distribution of ¹³C/¹²C isotope ratios within plant metabolites, which can be determined by isotopic ¹³C NMR spectrometry. It focuses on the key metabolite glucose and on enzyme activities and physiological processes that are responsible for the carbon isotope patterns in glucose from different biological origins. It further considers how intramolecular ¹³C/¹²C isotope ratios in glucose can be exploited to understand fundamental aspects of plant biological chemistry, how these are related to environmental parameters and how these influence metabolites beyond central sugar metabolism. It does not purport to be an extensive overview of intramolecular isotopic patterns. Rather, the aim is to show how a full understanding of ¹³C/¹²C fractionations occurring during plant metabolism can only be possible once the factors that define intramolecular isotope values are better delineated.
authors.affiliation: Group for the Elucidation of Biosynthesis by Isotopic Spectrometry, Interdisciplinary Chemistry: Synthesis, Analysis, Modelling-CEISAM, University of Nantes-CNRS UMR 6230, BP 92208, F-44322, Nantes, France.
authors.forename: Alexis
authors.initials: A
authors.lastname: Gilbert
authors.forename: Virginie
authors.initials: V
authors.lastname: Silvestre
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Robins
authors.forename: Gérald S
authors.initials: GS
authors.lastname: Remaud
authors.forename: Guillaume
authors.initials: G
authors.lastname: Tcherkez
mesh_terms: D002247:Carbon Isotopes / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D015394:Molecular Structure; D013395:Sucrose / Q000032:analysis / Q000737:chemistry*
chemical_list: D002247:Carbon Isotopes; D013395:Sucrose