title: Microbiologic profile of endodontic infections from HIV- and HIV+ patients using multiple-displacement amplification and checkerboard DNA-DNA hybridization.
abstract: OBJECTIVE
  To compare the microbiota of endodontic infections in necrotic pulp from HIV-negative and HIV-positive subjects.
  
  
  METHODS
  Root canal samples from necrotic pulp were collected from 40 HIV- and 20 HIV+ subjects. Pulps were amplified using multiple displacement amplification (MDA). Then, checkerboard DNA-DNA hybridization was employed to assess the levels of 107 microbial taxa. The percentage of DNA probe count and the percentage of teeth colonized by each test species were investigated. Significant differences between groups regarding proportions of taxa and prevalence of the test species were sought using the Mann-Whitney test and the Chi-square analysis, respectively.
  
  
  RESULTS
  The most prevalent taxa detected were Dialister pneumosintes, Stenotrophomonas maltophilia, Streptococcus sobrinus, Corynebacterium diphteriae, and Helicobacter pylori among HIV- subjects and D. pneumosintes, Prevotella tannerae, Porphyromonas gingivalis, Parvimonas micra, Prevotella nigrescens, and Corynebacterium diphtheriae among HIV+ individuals. D. pneumosintes, C. diphtheria, and C. albicans were the most abundant species in the HIV- group, whereas the predominant taxa in HIV+ samples were P. tannerae, D. pneumosintes and Olsenella uli. P. tannerae, O. uli, Veilonella dispar, Bacteroides fragilis, and Actinomyces meyeri were significantly more abundant in HIV+ samples.
  
  
  CONCLUSIONS
  There were significant differences in the prevalence and proportions of specific microbial taxa between HIV- and HIV+ individuals. The root canal microbiota may represent a reservoir of important oral and medical pathogens, mainly in HIV+ individuals.
authors.affiliation: Department of Operative Dentistry, School of Dentistry, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: L C N
authors.initials: LC
authors.lastname: Brito
authors.forename: A P Ribeiro
authors.initials: AP
authors.lastname: Sobrinho
authors.forename: R P
authors.initials: RP
authors.lastname: Teles
authors.forename: S S
authors.initials: SS
authors.lastname: Socransky
authors.forename: A D
authors.initials: AD
authors.lastname: Haffajee
authors.forename: L Q
authors.initials: LQ
authors.lastname: Vieira
authors.forename: F R F
authors.initials: FR
authors.lastname: Teles
mesh_terms: D000190:Actinomyces / Q000302:isolation & purification; D000293:Adolescent; D000328:Adult; D001419:Bacteria / Q000145:classification*; D001441:Bacteroides fragilis / Q000302:isolation & purification; D002176:Candida albicans / Q000302:isolation & purification; D002648:Child; D003353:Corynebacterium diphtheriae / Q000302:isolation & purification; D015342:DNA Probes; D003786:Dental Pulp Cavity / Q000382:microbiology; D003790:Dental Pulp Necrosis / Q000382:microbiology*; D005260:Female; D016965:Gram-Negative Anaerobic Straight, Curved, and Helical Rods / Q000145:classification; D018023:HIV Seronegativity; D006679:HIV Seropositivity / Q000382:microbiology*; D016480:Helicobacter pylori; D006801:Humans; D008297:Male; D008875:Middle Aged; D021141:Nucleic Acid Amplification Techniques; D009693:Nucleic Acid Hybridization / Q000379:methods; D010462:Peptostreptococcus / Q000302:isolation & purification; D016966:Porphyromonas gingivalis / Q000302:isolation & purification; D018720:Prevotella / Q000145:classification; D045242:Prevotella nigrescens / Q000302:isolation & purification; D020615:Stenotrophomonas maltophilia / Q000302:isolation & purification; D016986:Streptococcus sobrinus / Q000302:isolation & purification; D014678:Veillonella / Q000302:isolation & purification; D055815:Young Adult
chemical_list: D015342:DNA Probes