title: [The change of telomere protein in BEAS-2B malignant transformation cell induced by coal tar pitch smoke extracts].
abstract: OBJECTIVE
  By testing the changes of telomere binding protein in malignant transformation BEAS-2B cells induced by coal tar pitch smoke extracts, to study the role of protection of telomeres 1 (POT1), telomeric repeat binding factor 1 (TRF1) and TRF2 in tumorgenesis that contact with coal tar pitch.
  
  
  METHODS
  The BEAS-2B cells were induced by coal tar pitch smoke extracts to form malignant transformation cell model in vitro. The gene expression levels of mRNA were assessed by real-time quantitative RT-PCR, the protein expression variations were determined by cell culture overslip of immunohistochemical methods.
  
  
  RESULTS
  In malignant transformation cells, the mRNA expression level (POT1: 0.63 ± 0.04, TRF1: 0.36 ± 0.01) and the protein expression level (POT1: 0.36 ± 0.05, TRF1: 0.09 ± 0.03) of POT1 and TRF1 was statistically significant decreased compared to that of BEAS-2B group (mRNA: POT1: 1.00 ± 0.04, TRF1: 1.01 ± 0.16; protein: POT1: 0.55 ± 0.07, TRF1: 0.27 ± 0.07) and DMSO group (mRNA: POT1: 0.89 ± 0.12, TRF1: 0.90 ± 0.08; protein: POT1: 0.55 ± 0.10, TRF1: 0.26 ± 0.04) (P < 0.05); mRNA expression level (1.45 ± 0.07) and the protein expression level (0.88 ± 0.06) of TRF2 was increased compared to that of BEAS-2B group (mRNA: 1.00 ± 0.07, protein: 0.48 ± 0.06) and DMSO group (mRNA: 1.00 ± 0.06, protein: 0.50 ± 0.06) (P < 0.05).
  
  
  CONCLUSIONS
  The change of gene and protein expression level in POT1, TRF1, and TRF2 involved in the process that evolved into malignant transformation in bronchial epithelial cells BEAS-2B induced by coal tar pitch smoke extracts.
authors.affiliation: College of Public Health, Zhengzhou University, Zhengzhou, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Zhi-Tao
authors.initials: ZT
authors.lastname: Li
authors.forename: Han-Song
authors.initials: HS
authors.lastname: Zhu
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhao
authors.forename: Li-Xia
authors.initials: LX
authors.lastname: Wang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Yan
authors.forename: I Shi-En
authors.initials: IS
authors.lastname: L
authors.forename: Dong
authors.initials: D
authors.lastname: Xu
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Wu
authors.forename: Yong-Jun
authors.initials: YJ
authors.lastname: Wu
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Wu
mesh_terms: D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000378:metabolism*; D003033:Coal Tar / Q000633:toxicity*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D012091:Repetitive Sequences, Nucleic Acid; D034501:Telomere-Binding Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D034501:Telomere-Binding Proteins; D003033:Coal Tar