title: Effect of simulated space gravity environment on Gibberella moniliformis EZG0807.
abstract: To determine the feasibility of inducing mutation for Gibberella moniliformis EZG0807 with a superconducting magnet, this paper investigated the effects of this instrument on the filamentous fungus G. moniliformis EZG0807. The superconducting magnet could simulate space gravity environment from hypo-gravity (0 g) to hyper-gravity (2 g). After G. moniliformis EZG0807 was exposed to the superconducting magnet for 72 h, the morphological observation, agar diffusion method, and amplified fragment length polymorphism were performed to detect the mutagenic effects in the aspect of morphology, the activity of metabolites, and genomic DNA, respectively. The mutant strain M7212 in 1 g (16 T) was different from the control in the morphology, showing no activity against the four tested bacteria Escherichia coli, Bacillus subtilis, Staphylococcus aureus, and Proteus vulgaris, and lost a size of 675 bp band on the genomic DNA. These results indicated that the superconducting magnet could be used to induce mutation for G. moniliformis EZG0807, which enabled improving the production of G. moniliformis EZG0807 and providing an effective approach for fungal breeding.
authors.affiliation: The Institute of Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors.forename: Jvfen
authors.initials: J
authors.lastname: Yan
authors.forename: Peng
authors.initials: P
authors.lastname: Shang
authors.forename: De
authors.initials: D
authors.lastname: Zheng
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Dong
authors.forename: Jun
authors.initials: J
authors.lastname: Mao
authors.forename: Suping
authors.initials: S
authors.lastname: Wang
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Shulin
authors.initials: S
authors.lastname: Yang
mesh_terms: D001412:Bacillus subtilis / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics; D005821:Genetic Techniques / Q000295:instrumentation*; D005874:Gibberella / Q000737:chemistry / Q000235:genetics* / Q000254:growth & development; D006112:Gravitation; D059346:Magnets; D016296:Mutagenesis; D009154:Mutation; D013211:Staphylococcus aureus / Q000737:chemistry / Q000235:genetics