title: Molecular identification of unilocular hydatid cysts from domestic ungulates in Ethiopia: implications for human infections.
abstract: To identify the etiologic agents of cystic echinococcosis in Ethiopia, unilocular hydatid cysts were collected from 11 sheep, 16 cattle and 16 camels slaughtered in abattoirs of Aweday, Jijiga, Haramaya and Addis Ababa during June 2010 to February 2011. A PCR-based DNA sequencing of the mitochondrial cytochrome oxidase c subunit 1 gene (cox1) was conducted for 40 cysts. The majority of cysts (87.5%) were identified as Echinococcus granulosus sensu stricto and the rest as Echinococcus canadensis. The fertile cysts of E. granulosus s.s. were found only from sheep, although it occurred in all the host species. The predominance of E. granulosus s.s. has important implications for public health since this species is the most typical causative agent of human cystic echinococcosis worldwide. The major cox1 haplotype of E. granulosus s.s. detected in Ethiopia was the same as that has been reported to be most common in Peru and China. However, a few cox1 haplotypes unique to Ethiopia were found in both of the two Echinococcus species. The present regional data would serve as baseline information in determining the local transmission patterns and in designing appropriate control strategies.
authors.affiliation: College of Veterinary Medicine, Haramaya University, P.O. Box 281, Dire Dawa, Ethiopia.
authors.forename: Zerihun
authors.initials: Z
authors.lastname: Hailemariam
authors.forename: Minoru
authors.initials: M
authors.lastname: Nakao
authors.forename: Sissay
authors.initials: S
authors.lastname: Menkir
authors.forename: Antti
authors.initials: A
authors.lastname: Lavikainen
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Yanagida
authors.forename: Munehiro
authors.initials: M
authors.lastname: Okamoto
authors.forename: Akira
authors.initials: A
authors.lastname: Ito
mesh_terms: D000003:Abattoirs; D000818:Animals; D001483:Base Sequence; D002162:Camelus / Q000469:parasitology*; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology*; D004443:Echinococcosis / Q000469:parasitology / Q000662:veterinary*; D004446:Echinococcus / Q000235:genetics / Q000302:isolation & purification*; D048209:Echinococcus granulosus / Q000235:genetics / Q000302:isolation & purification*; D005002:Ethiopia; D006239:Haplotypes; D006801:Humans; D008969:Molecular Sequence Data; D011634:Public Health; D017422:Sequence Analysis, DNA; D012756:Sheep; D012757:Sheep Diseases / Q000469:parasitology*