title: [Effects of emdogain on human periodontal ligament cells in vitro].
abstract: OBJECTIVE
  To investigate the effects of emdogain, enamel matrix derivative (EMD), on the proliferation, cell cycle, mineralization and ultrastructure of  human periodontal ligament (PDL) cells in vitro.
  
  
  METHODS
  The influence of cell growth on PDL cells was evaluated by Cell Counting Kit-8 (CCK-8) in the  presence and absence of emdogain, after 1, 3, and 5 d of culture. DNA synthesis and ultrastructure of PDL cells were observed by flow cytometry(FCM)  and transmission electron microscopy (TEM) in the presence and absence of emdogain after 3 d of culture. The increasing of osteogenic capacity was  verified by the expression changes of osteogenic differentiation markers of bone sialoprotein (BSP) and osteopontin (OPN) in emdogain-treated PDL  cells by immunohistochemicl staining.
  
  
  RESULTS
  Incubation of PDL cells with emdogain after 3 d significantly stimulated cell growth and DNA synthesis.  Emdogain enhanced the osteogenic potential of PDL cells by high expression of osteogenic differentiation markers of BSP and OPN.
  
  
  CONCLUSIONS
  The data indicate that Emdogain enhances cell proliferation and promotes differentiation of PDL cells, which contributes to periodontal tissue  regeneration .
authors.affiliation: Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing 100081, China.
authors.forename: Feng-qiu
authors.initials: FQ
authors.lastname: Zhang
authors.forename: Huan-xin
authors.initials: HX
authors.lastname: Meng
authors.forename: Jie
authors.initials: J
authors.lastname: Han
authors.forename: Kai-ning
authors.initials: KN
authors.lastname: Liu
mesh_terms: D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D003746:Dental Enamel Proteins / Q000494:pharmacology*; D005434:Flow Cytometry; D006801:Humans; D058574:Integrin-Binding Sialoprotein / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D053495:Osteopontin / Q000378:metabolism; D010513:Periodontal Ligament / Q000166:cytology* / Q000648:ultrastructure
chemical_list: D003746:Dental Enamel Proteins; D058574:Integrin-Binding Sialoprotein; C109069:enamel matrix proteins; D053495:Osteopontin; D004247:DNA