title: Dynex: multiplex ELISA technology.
abstract: Conventional enzyme-linked immunosorbent assay (ELISA) is a gold standard for screening antibodies and testing for protein or antigen presence. A significant limitation of this assay resides in the fact that only one analyte can be assessed per microplate well. Here, we describe and investigate a new technology consisting of an automated ELISA system in which up to 10 analytes can be measured within one single well, thus improving productivity, accuracy, and repeatability by reducing the amount of human labor required. Another strength of the platform is that a user can load any necessary sets/subsets of beads to perform required assays, with improved flexibility compared to manufactured-loaded arrays for multiplex analysis. We also demonstrate that this system can be used to determine the pathogenicity (i.e., presence of Shiga toxins) and serotype (i.e., Escherichia coli O157) of E. coli isolates.
authors.affiliation: USDA-ARS, Foodborne Contaminants Research Unit, Albany, CA 94710, USA.
authors.forename: Laurie M
authors.initials: LM
authors.lastname: Clotilde
authors.forename: Clay
authors.initials: C
authors.lastname: Bernard
authors.forename: Dean E
authors.initials: DE
authors.lastname: Sequera
authors.forename: Anis
authors.initials: A
authors.lastname: Karmali
authors.forename: Andrew
authors.initials: A
authors.lastname: Fusellier
authors.forename: J Mark
authors.initials: JM
authors.lastname: Carter
mesh_terms: D057205:Automation, Laboratory / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D019453:Escherichia coli O157 / Q000472:pathogenicity*; D006801:Humans; D013677:Medical Laboratory Science / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D022603:Shiga Toxins / Q000032:analysis*
chemical_list: D022603:Shiga Toxins