title: Prevalence of OXA-type β-lactamases among Acinetobacter baumannii isolates from Northwest of Iran.
abstract: Carbapenems have been considered as last line antibiotics for treatment of multidrug-resistant (MDR) Acinetobacter baumannii but carbapenem resistant A. baumannii has been increased during the last decade in many parts of the world. OXA-type β-lactamase enzymes are the most common cause of carbapenem resistance in A. baumannii and presence of ISAba1 in upstream of these genes may increase the expression of these OXA genes. The aim of this study was to determine, for the first time, the antibiotic resistance pattern and prevalence of OXA type β-lactamases among nosocomial A. baumannii isolates from northwest of Iran. A total of 100 A. baumannii isolates were recovered from hospitalized patients in a university hospital in northwest of Iran. Sixty-two percent of isolates were resistant to imipenem. All isolates carried bla(OXA-51)-like gene. Among imipenem resistant isolates, 88.7% carried bla(OXA-23)-like, 1.6% carried bla(OXA-40)-like, and 3.2% had bla(OXA-58)-like resistance genes. Ninety percent of isolates contained ISAba1 element and in 74.2% of imipenem resistant isolates, ISAba1 was located in upstream of bla(OXA-23)-like. The results of this study demonstrated high prevalence of OXA-type carbapenemase among MDR A. bumanii in the Northwest of Iran.
authors.affiliation: Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Nasrollah
authors.initials: N
authors.lastname: Sohrabi
authors.forename: Safar
authors.initials: S
authors.lastname: Farajnia
authors.forename: Mohammad Taghi
authors.initials: MT
authors.lastname: Akhi
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Nahaei
authors.forename: Behrooz
authors.initials: B
authors.lastname: Naghili
authors.forename: Amir
authors.initials: A
authors.lastname: Peymani
authors.forename: Zohreh
authors.initials: Z
authors.lastname: Amiri
authors.forename: Mohammad Ahangarzadeh
authors.initials: MA
authors.lastname: Rezaee
authors.forename: Nazli
authors.initials: N
authors.lastname: Saeedi
mesh_terms: D000151:Acinetobacter Infections / Q000382:microbiology*; D040981:Acinetobacter baumannii / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D000328:Adult; D000368:Aged; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015373:Bacterial Typing Techniques; D015780:Carbapenems / Q000494:pharmacology*; D004251:DNA Transposable Elements; D005260:Female; D005798:Genes, Bacterial; D006801:Humans; D007492:Iran; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D010957:Plasmids; D016133:Polymerase Chain Reaction; D018440:beta-Lactam Resistance / Q000187:drug effects / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D015780:Carbapenems; D004251:DNA Transposable Elements; D001618:beta-Lactamases