title: Functional Studies of microRNAs in Neural Stem Cells: Problems and Perspectives.
abstract: In adult mammals, neural stem cells (NSCs) are found in two niches of the brain; the subventricular zone by the lateral ventricles and the subgranular zone of the dentate gyrus in the hippocampus. Neurogenesis is a complex process that is tightly controlled on a molecular level. Recently, microRNAs (miRNAs) have been implicated to play a central role in the regulation of NCSs. miRNAs are small, endogenously expressed RNAs that regulate gene expression at the post-transcriptional level. However, functional studies of miRNAs are complicated due to current technical limitations. In this review we describe recent findings about miRNAs in NSCs looking closely at miR-124, miR-9, and let-7. In addition, we highlight technical strategies used to investigate miRNA function, accentuating limitations, and potentials.
authors.affiliation: Laboratory of Molecular Neurogenetics, Department of Experimental Medical Science, Wallenberg Neuroscience Center, Lund University Lund, Sweden.
authors.forename: Malin
authors.initials: M
authors.lastname: Akerblom
authors.forename: Rohit
authors.initials: R
authors.lastname: Sachdeva
authors.forename: Johan
authors.initials: J
authors.lastname: Jakobsson