title: Alteration of in vitro and acute in vivo toxicity of textile dyeing wastewater after chemical and biological remediation.
abstract: BACKGROUND
  Textile industry is one of the most common and essential sectors in Tunisia. However, the treatment of textile effluents becomes a university because of their toxic impacts on waters, soils, flora, and fauna.
  
  
  METHODS
  The aim of this work was to evaluate the ability of Pseudomonas putida mt-2 to decolorize a textile wastewater and to compare the biologic decolorization process to the chemical one currently used by the textile industry.
  
  
  RESULTS
  P. putida exhibited a high decolorizing capacity of the studied effluent, compared to the coagulation-flocculation method with decolorization percentage of 86% and 34.5%, respectively. Genotoxicity of the studied effluent, before and after decolorization by P. putida mt-2, was evaluated in vitro, using the SOS chromotest, and in vivo, in mouse bone marrow, by assessing the percentage of cells bearing different chromosome aberrations compared to not treated mice. In addition, textile effluent statistically significant influenced acetylcholinesterase and butyrylcholinesterase activities and lipid peroxidation (p < 0.01) when compared to not-treated mice. Coagulation-flocculation treatment process used by industry was revealed to be ineffective. Indeed toxicities persisted after treatment and the effluent did not show any statistically significant decrease in toxicities compared to non-treated effluent. Our results indicate that P. putida is a promising and improved alternative to treating industrial scale effluent compared to current chemical decolorization procedures used by the Tunisian textile industry.
authors.affiliation: Equipe de Recherche en Physico-chimie et Biotechnologie (E.R.P.C.B-EA3914), IUT-UFR Sciences, Université de Caen-Basse, Normandie, France.
authors.forename: Hedi
authors.initials: H
authors.lastname: Ben Mansour
authors.forename: Ikram
authors.initials: I
authors.lastname: Houas
authors.forename: Fadoua
authors.initials: F
authors.lastname: Montassar
authors.forename: Kamel
authors.initials: K
authors.lastname: Ghedira
authors.forename: Daniel
authors.initials: D
authors.lastname: Barillier
authors.forename: Ridha
authors.initials: R
authors.lastname: Mosrati
authors.forename: Leila
authors.initials: L
authors.lastname: Chekir-Ghedira
mesh_terms: D000818:Animals; D001419:Bacteria / Q000187:drug effects / Q000235:genetics; D001673:Biodegradation, Environmental; D002800:Cholinesterase Inhibitors; D002869:Chromosome Aberrations / Q000139:chemically induced; D004396:Coloring Agents / Q000737:chemistry / Q000633:toxicity*; D005260:Female; D007220:Industrial Waste / Q000009:adverse effects* / Q000032:analysis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013783:Textile Industry; D013997:Time Factors; D018675:Toxicity Tests; D014865:Waste Disposal, Fluid / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000633:toxicity*; D015025:Zearalenone / Q000633:toxicity
chemical_list: D002800:Cholinesterase Inhibitors; D004396:Coloring Agents; D007220:Industrial Waste; D014874:Water Pollutants, Chemical; D015025:Zearalenone