title: Site specific chemoselective labelling of proteins with robust and highly sensitive Ru(II) bathophenanthroline complexes.
abstract: The bioorthogonal and chemoselective fluorescence labelling of several cell-free synthesized proteins containing a site-specifically incorporated azido amino acid was possible using different alkyne-functionalized Ru(II) bathophenanthroline complexes. We were able to achieve a selective labelling even in complex mixtures of proteins despite the fact that ruthenium dyes normally show a high tendency for unspecific interactions with proteins and are commonly used for total staining of proteins. Since the employed Ru complexes are extremely robust, photo-stable and highly sensitive, the approach should be applicable to the production of labelled proteins for single molecule spectroscopy and fluorescence-based interaction studies.
authors.affiliation: Institut für Organische Chemie und Biochemie, Albert-Ludwigs-Universität Freiburg, Freiburg 79104, Germany.
authors.forename: Matthew C
authors.initials: MC
authors.lastname: Uzagare
authors.forename: Iris
authors.initials: I
authors.lastname: Claussnitzer
authors.forename: Michael
authors.initials: M
authors.lastname: Gerrits
authors.forename: Willi
authors.initials: W
authors.lastname: Bannwarth
mesh_terms: D015394:Molecular Structure; D010618:Phenanthrolines / Q000737:chemistry*; D011506:Proteins / Q000032:analysis / Q000737:chemistry*; D012428:Ruthenium / Q000737:chemistry*
chemical_list: D010618:Phenanthrolines; D011506:Proteins; C006686:bathophenanthroline; D012428:Ruthenium