title: [The role of amino acid sequence between 551 and 565 in the cytoplasmic domain of glycoprotein (GP) I b alpha in the regulation of the VWF binding to GP I b alpha].
abstract: OBJECTIVE
  To explore the role of the amino acids between 551 and 565 in the cytoplasmic domain of glycoprotein (GP) I b alpha in the VWF binding to GP I b alpha.
  
  
  METHODS
  The VWF binding to GP I b alpha induced by ristocetin was analyzed by flow cytometry, in three GP I b-IX-expressing Chinese hamster ovary (CHO) cell lines 1b9, delta 565 and delta 551, adhesion of above cells on VWF by flow chamber analysis at shear rate of 200 s(-1). The spread of GP I b-IX-expressing cells were stimulated with botrocetin on VWF-coated coverslips by confocal microscope.
  
  
  RESULTS
  The VWF binding to GP I b alpha was higher in delta 565 cells stimulated by ristocetin than in delta 551 or 1b9 cells. The number of delta 565 cells adhered on the VWF-coated-chamber was more than that of controls at shear rate of 200 s(-1). Moreover, the surface spreading areas of delta 565 cells were greater than that of the controls on VWF-coated coverslips.
  
  
  CONCLUSIONS
  The amino acids between 551 and 565 in the cytoplasmic domain of GP I b alpha regulates the VWF binding to GP I b alpha.
authors.affiliation: School of Biological Science and Medical Engineering, Beijing University of Aeronautics and Astronautics, Beijing 100191, China.
authors.forename: Wei-lin
authors.initials: WL
authors.lastname: Zhang
authors.forename: Yi
authors.initials: Y
authors.lastname: Liao
authors.forename: Yan-hong
authors.initials: YH
authors.lastname: Yuan
authors.forename: Rong
authors.initials: R
authors.lastname: Yan
authors.forename: Chang-geng
authors.initials: CG
authors.lastname: Ruan
authors.forename: Ke-sheng
authors.initials: KS
authors.lastname: Dai
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D010973:Platelet Adhesiveness; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000235:genetics* / Q000378:metabolism*; D014841:von Willebrand Factor / Q000378:metabolism*
chemical_list: D019038:Platelet Glycoprotein GPIb-IX Complex; D014841:von Willebrand Factor