title: Fast real-time PCR for the detection of crustacean allergen in foods.
abstract: Crustaceans are one of the most common allergens causing severe food reaction. These food allergens are a health problem, and they have become very important; there are various regulations that establish that labeling must be present regarding these allergens to warn consumers. In the present work a fast real-time PCR, by a LNA probe, was developed. This allows the detection of crustaceans in all kinds of products, including processed products in which very aggressive treatments of temperature and pressure during the manufacturing process are used. This methodology provides greater sensitivity and specificity and reduces the analysis time of real-time PCR to 40 min. This methodology was further validated by means of simulating products likely to contain this allergen. For this, products present on the market were spiked with crustacean cooking water. The assay is a potential tool in issues related to the labeling of products and food security to protect the allergic consumer.
authors.affiliation: Area of Molecular Biology and Biotechnology, ANFACO-CECOPESCA, Vigo, 36310 Pontevedra, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Herrero
authors.forename: Juan M
authors.initials: JM
authors.lastname: Vieites
authors.forename: Montserrat
authors.initials: M
authors.lastname: Espiñeira
mesh_terms: D000485:Allergens / Q000009:adverse effects / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D060829:Arthropod Proteins / Q000009:adverse effects / Q000235:genetics / Q000378:metabolism*; D004044:Dietary Proteins / Q000009:adverse effects / Q000378:metabolism*; D005512:Food Hypersensitivity / Q000517:prevention & control; D006801:Humans; D060888:Real-Time Polymerase Chain Reaction; D012758:Shellfish / Q000009:adverse effects / Q000032:analysis
chemical_list: D000485:Allergens; D060829:Arthropod Proteins; D004044:Dietary Proteins