title: Antibody monitoring system to support the single-antigen Luminex assay in donor-specific antibody detection.
abstract: The antibody monitoring system (AMS) is a crossmatch test using enzyme-linked immunoassay to detect donor-specific anti-HLA antibodies (DSA). The aim of this study was to determine whether the AMS assay is useful for supporting DSA results measured with single-antigen Luminex assay (Luminex-DSA) in renal transplant recipients. Thirty sera from 12 sensitized recipients and 71 sera from 60 nonsensitized recipients were screened by complement-dependent cytotoxic crossmatch (CDC-XM), flow cytometric crossmatch (FCXM), AMS, and the Luminex assays. Twenty-two sera (73.3%) from sensitized patients were positive for Luminex-DSA, and the AMS assay revealed the great specificity and positive predictive value for detecting Luminex-DSA in sera from sensitized patients. Positivity in the AMS assay was observed most frequently in Luminex-DSA-positive sera with ≥10,000 mean fluorescence intensity (MFI) levels (66.7%). Of the 101 sera, the results of the AMS assay were compatible with CDC-XM results in 73 sera (91.3%) and with FCXM results in 72 sera (90.0%); these rates were significantly higher for the AMS assay than for the Luminex assay (p = 0.008 for CDC-XM and p = 0.001 for FCXM). In conclusion, the AMS assay is useful as a supportive solid-phase method for predicting actual crossmatch and high level of Luminex-DSA in sensitized patients.
authors.affiliation: Division of Nephrology, Department of Internal Medicine, The Catholic University of Korea, Seoul, Korea.
authors.forename: Hyeon Seok
authors.initials: HS
authors.lastname: Hwang
authors.forename: In O
authors.initials: IO
authors.lastname: Sun
authors.forename: Hye Eun
authors.initials: HE
authors.lastname: Yoon
authors.forename: Bum Soon
authors.initials: BS
authors.lastname: Choi
authors.forename: Eun Jee
authors.initials: EJ
authors.lastname: Oh
authors.forename: Ji Il
authors.initials: JI
authors.lastname: Kim
authors.forename: In Sung
authors.initials: IS
authors.lastname: Moon
authors.forename: Yong Soo
authors.initials: YS
authors.lastname: Kim
authors.forename: Chul Woo
authors.initials: CW
authors.lastname: Yang
mesh_terms: D000328:Adult; D000906:Antibodies / Q000097:blood*; D005260:Female; D006680:HLA Antigens / Q000276:immunology*; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D016030:Kidney Transplantation / Q000276:immunology; D008297:Male; D008875:Middle Aged; D012372:ROC Curve; D014019:Tissue Donors
chemical_list: D000906:Antibodies; D006680:HLA Antigens