title: Comparison of plasma from healthy nonsmokers, smokers, and lung cancer patients: pattern-based differentiation profiling of low molecular weight proteins and peptides by magnetic bead technology with MALDI-TOF MS.
abstract: BACKGROUND
  Smoking is the major contributor of lung cancer (LC), which accounts for millions of death.
  
  
  OBJECTIVE
  This study focused on the correlation between the proteomic profiling of LC patients, and healthy nonsmokers and smokers.
  
  
  METHODS
  Pattern-based peptide profiling of 186 plasma samples was performed through reversed-phase chromatography-18 magnetic bead fractionation coupled with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis and resulted data were evaluated statistically by ClinProTool.
  
  
  RESULTS
  Marker peaks at m/z 1760, 5773, 5851, 2940, and 7172 were found with an excellent statistical figure.
  
  
  CONCLUSIONS
  Selected marker peaks can be served as a differentiated tool of LC patients with high sensitivity and specificity.
authors.affiliation: Dr. Panjwani Center for Molecular Medicine and Drug Research, University of Karachi, Karachi, Pakistan. musharraf1977@yahoo.com
authors.forename: Syed G
authors.initials: SG
authors.lastname: Musharraf
authors.forename: Naghma
authors.initials: N
authors.lastname: Hashmi
authors.forename: M Iqbal
authors.initials: MI
authors.lastname: Choudhary
authors.forename: Nadeem
authors.initials: N
authors.lastname: Rizvi
authors.forename: Ahmed
authors.initials: A
authors.lastname: Usman
authors.lastname: Atta-ur-Rahman
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood*; D009363:Neoplasm Proteins / Q000097:blood*; D012907:Smoking / Q000097:blood*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins