title: The serogroup A capsular polysaccharide from Neisseria meningitidis enhances the cell-mediated immunity and the protective capacity induced by a dengue fusion protein in mice.
abstract: We previously tested in monkeys the P64k-DomIII fusion protein of DEN-2 (PD5), combined with the serogroup A capsular polysaccharide (CPS-A) from N. meningitidis as an immunopotentiator. The results revealed the induction of neutralizing antibodies and partial protection after DEN-2 challenge. Since one formulation of the CPS-A was only evaluated in monkeys, in the present study, we evaluated two CPS-A-based formulations in mice. Animals immunized with PD5 in alum with the highest dose of CPS-A produced the highest levels of INF-γ secretion upon viral stimulation, and accordingly, 100% protection. This is the first report that describes the dose effect of CPS-A and its capacity to potentiate the cell-mediated immunity induced by a heterologous antigen in mice.
authors.affiliation: Vaccine Division, Center for Genetic Engineering and Biotechnology (CIGB), Avenue 31, P.O. Box 6162, Havana 6, 10 600, Cuba. lisset.hermida@cigb.edu.cu
authors.forename: Lisset
authors.initials: L
authors.lastname: Hermida
authors.forename: Iris
authors.initials: I
authors.lastname: Valdés
authors.forename: Lázaro
authors.initials: L
authors.lastname: Gil
authors.forename: Lídice
authors.initials: L
authors.lastname: Bernardo
authors.forename: Laura
authors.initials: L
authors.lastname: Lazo
authors.forename: Yaremis
authors.initials: Y
authors.lastname: Romero
authors.forename: María G
authors.initials: MG
authors.lastname: Guzmán
authors.forename: Gerardo
authors.initials: G
authors.lastname: Guillén
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D016667:Bacterial Capsules / Q000008:administration & dosage / Q000276:immunology*; D003715:Dengue / Q000276:immunology* / Q000517:prevention & control* / Q000821:virology; D003716:Dengue Virus / Q000235:genetics / Q000276:immunology*; D005260:Female; D006801:Humans; D007111:Immunity, Cellular; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D009345:Neisseria meningitidis / Q000145:classification / Q000235:genetics / Q000276:immunology*; D015854:Up-Regulation; D014760:Viral Fusion Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D014760:Viral Fusion Proteins