title: Mycobacterium minnesotense sp. nov., a photochromogenic bacterium isolated from sphagnum peat bogs.
abstract: Several intermediate-growing, photochromogenic bacteria were isolated from sphagnum peat bogs in northern Minnesota, USA. Acid-fast staining and 16S rRNA gene sequence analysis placed these environmental isolates in the genus Mycobacterium, and colony morphologies and PCR restriction analysis patterns of the isolates were similar. Partial sequences of hsp65 and dnaJ1 from these isolates showed that Mycobacterium arupense ATCC BAA-1242(T) was the closest mycobacterial relative, and common biochemical characteristics and antibiotic susceptibilities existed between the isolates and M. arupense ATCC BAA-1242(T). However, compared to nonchromogenic M. arupense ATCC BAA-1242(T), the environmental isolates were photochromogenic, had a different mycolic acid profile and had reduced cell-surface hydrophobicity in liquid culture. The data reported here support the conclusion that the isolates are representatives of a novel mycobacterial species, for which the name Mycobacterium minnesotense sp. nov. is proposed. The type strain is DL49(T) (=DSM 45633(T) = JCM 17932(T) = NCCB 100399(T)).
authors.affiliation: Department of Biology, University of Minnesota Duluth, Duluth, MN 55812, USA.
authors.forename: Geoffrey D
authors.initials: GD
authors.lastname: Hannigan
authors.affiliation: Department of Biology, University of Minnesota Duluth, Duluth, MN 55812, USA.
authors.forename: Bogdana
authors.initials: B
authors.lastname: Krivogorsky
authors.affiliation: Department of Biology, University of Minnesota Duluth, Duluth, MN 55812, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Fordice
authors.affiliation: Department of Biology, University of Minnesota Duluth, Duluth, MN 55812, USA.
authors.forename: Jacqueline B
authors.initials: JB
authors.lastname: Welch
authors.affiliation: Department of Biology, University of Minnesota Duluth, Duluth, MN 55812, USA.
authors.forename: John L
authors.initials: JL
authors.lastname: Dahl
mesh_terms: D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D008910:Minnesota; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D009171:Mycolic Acids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D044003:Sphagnopsida / Q000382:microbiology*; D053833:Wetlands
chemical_list: D004269:DNA, Bacterial; D009171:Mycolic Acids; D012336:RNA, Ribosomal, 16S