title: Lysozyme-loaded, electrospun chitosan-based nanofiber mats for wound healing.
abstract: In this study, a blend mixture of chitosan-ethylenediaminetetraacetic acid (CS 2 wt%-EDTA) at a weight ratio of 30/70 and polyvinyl alcohol (PVA) solution (10 wt%) was electrospun to produce fibrous mats with lysozyme (10, 20 and 30 wt%) used for wound healing. The morphology and diameter of the electrospun fiber mats with and without lysozyme were analyzed by scanning electron microscopy (SEM). The amount of lysozyme loaded in the nanofiber mats was measured by HPLC. The cell lysis activity of the lysozyme was investigated with Micrococcus lysodeikticus cells as a substrate. The wound healing activity was performed in vivo using male Wistar rats. The SEM images of all lysozyme-loaded fibers show a smooth fiber without beads with an average diameter of 143-209 nm. The amount of lysozyme loaded in the nanofiber mats was slightly decreased when the initial concentration of lysozyme was increased. The rapid lysozyme release from the nanofiber mats was obtained and is dependent on the lysozyme-loading amount. In animal wound healing, lysozyme loaded CS-EDTA nanofiber mats accelerated the rate of wound healing when compared to the controls (gauze). In conclusion, our experiments demonstrated that biomaterials composed of lysozyme loaded CS-EDTA nanofibers have a potential for wound healing.
authors.affiliation: Pharmaceutical Development of Green Innovations Group, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand.
authors.forename: Natthan
authors.initials: N
authors.lastname: Charernsriwilaiwat
authors.forename: Praneet
authors.initials: P
authors.lastname: Opanasopit
authors.forename: Theerasak
authors.initials: T
authors.lastname: Rojanarata
authors.forename: Tanasait
authors.initials: T
authors.lastname: Ngawhirunpat
mesh_terms: D000818:Animals; D001458:Bandages; D002152:Calorimetry, Differential Scanning; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D004396:Coloring Agents; D004492:Edetic Acid; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D008297:Male; D008837:Micrococcus / Q000187:drug effects; D008855:Microscopy, Electron, Scanning; D009113:Muramidase / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology*; D057139:Nanofibers / Q000737:chemistry*; D011142:Polyvinyl Alcohol; D051381:Rats; D017208:Rats, Wistar; D012995:Solubility; D013778:Tetrazolium Salts; D013844:Thiazoles; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D004396:Coloring Agents; D013778:Tetrazolium Salts; D013844:Thiazoles; D011142:Polyvinyl Alcohol; D048271:Chitosan; D004492:Edetic Acid; D009113:Muramidase; C022616:thiazolyl blue