title: Phosphorylation of CRN2 by CK2 regulates F-actin and Arp2/3 interaction and inhibits cell migration.
abstract: CRN2 (synonyms: coronin 1C, coronin 3) functions in the re-organization of the actin network and is implicated in cellular processes like protrusion formation, secretion, migration and invasion. We demonstrate that CRN2 is a binding partner and substrate of protein kinase CK2, which phosphorylates CRN2 at S463 in its C-terminal coiled coil domain. Phosphomimetic S463D CRN2 loses the wild-type CRN2 ability to inhibit actin polymerization, to bundle F-actin, and to bind to the Arp2/3 complex. As a consequence, S463D mutant CRN2 changes the morphology of the F-actin network in the front of lamellipodia. Our data imply that CK2-dependent phosphorylation of CRN2 is involved in the modulation of the local morphology of complex actin structures and thereby inhibits cell migration.
authors.forename: Charles-Peter
authors.initials: CP
authors.lastname: Xavier
authors.forename: Raphael H
authors.initials: RH
authors.lastname: Rastetter
authors.forename: Margit
authors.initials: M
authors.lastname: Blömacher
authors.forename: Maria
authors.initials: M
authors.lastname: Stumpf
authors.forename: Mirko
authors.initials: M
authors.lastname: Himmel
authors.forename: Reginald O
authors.initials: RO
authors.lastname: Morgan
authors.forename: Maria-Pilar
authors.initials: MP
authors.lastname: Fernandez
authors.forename: Conan
authors.initials: C
authors.lastname: Wang
authors.forename: Asiah
authors.initials: A
authors.lastname: Osman
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Miyata
authors.forename: Ruth A
authors.initials: RA
authors.lastname: Gjerset
authors.forename: Ludwig
authors.initials: L
authors.lastname: Eichinger
authors.forename: Andreas
authors.initials: A
authors.lastname: Hofmann
authors.forename: Stefan
authors.initials: S
authors.lastname: Linder
authors.forename: Angelika A
authors.initials: AA
authors.lastname: Noegel
authors.forename: Christoph S
authors.initials: CS
authors.lastname: Clemen