title: [Effects of pioglitazone on mitochondrial membrane potential of neonate rat's myocardial cells after hypoxia/reoxygenation].
abstract: OBJECTIVE
  To explore the effectiveness and mechanism of pioglitazone on mitochondrial membrane potential of neonate rat's myocardial cells after hypoxia/reoxygenation.
  
  
  METHODS
  Primary cultured myocardial cells of neonate Sprague-Dawley rats were pretreated with different concentrations of pioglitazone, pioglitazone's inhibitor and Chelerythrine, an inhibitor of protein kinase C (PKC). Hypoxia/reoxygenation model was established after 24 h of pretreatment. Subsequently, the mitochondrial membrane potential was detected with JC-1 staining under laser confocal microscopy during reoxygenation phase.
  
  
  RESULTS
  Compared with the control group, red/green fluorescent ratio of myocardial cells in the H-r model group decreased significantly after hypoxia/reoxygenation (red/green fluorescent ratio reduced from 1.20 +/- 0.05 to 1.13 +/- 0.02, P<0.01 ), and also decreased in 0.1 micromol/ L, 1 micromol/L and 2 micromol/L pioglitazone group (1.11 +/- 0.01, 1.10 +/- 0.01, 1.16 +/- 0.03, P<0.01, respectively). The red/green fluorescent ratio in pioglitazone + GW9662 group (1.12 +/- 0.02) and pioglitazone + Chelerythrine group (1.07 +/- 0.01) were both significantly lower than those in 2 micromol/L pioglitazone group (P<0.01).
  
  
  CONCLUSIONS
  The mitochondrial membrane potential of neonate rat's myocardial cells was descend after hypoxia/ reoxygenation, while pioglitazone can interrupt the process, indicating that the activation may be relevant to peroxisome proliferator-activated receptor gamma (PPARgamma) ligand and PKC pathway.
authors.affiliation: The Second Department of Geriatric Cardiology,Chinese People's Liberation Army Gerenal Hospital, Beijing 100853, China.
authors.forename: Qi-wei
authors.initials: QW
authors.lastname: Zhu
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.forename: Ping
authors.initials: P
authors.lastname: Ye
authors.forename: Lei-ming
authors.initials: LM
authors.lastname: Luo
authors.forename: Jin-yao
authors.initials: JY
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015687:Cell Hypoxia / Q000502:physiology; D002478:Cells, Cultured; D005260:Female; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects*; D015428:Myocardial Reperfusion Injury / Q000503:physiopathology / Q000517:prevention & control*; D032383:Myocytes, Cardiac / Q000473:pathology / Q000502:physiology*; D047495:PPAR gamma / Q000378:metabolism; D000077205:Pioglitazone; D011493:Protein Kinase C / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D045162:Thiazolidinediones / Q000494:pharmacology*
chemical_list: D047495:PPAR gamma; D045162:Thiazolidinediones; D011493:Protein Kinase C; D000077205:Pioglitazone