title: Astragalin attenuates lipopolysaccharide-induced inflammatory responses by down-regulating NF-κB signaling pathway.
abstract: Astragalin (AG), a flavonoid from many traditional herbs and medicinal plants, has been described to exhibit in vitro anti-inflammatory activity. The present study aimed to determine the protective effects and the underlying mechanisms of astragalin on lipopolysaccharide-induced endotoxemia and lung injury in mice. Mice were injected intraperitoneally (i.p.) with lipopolysaccharide (LPS) (dose range: 5-40 mg/kg). We observed mice on mortality for 7 days twice a day and recorded survival rates. In drug testing, we examined the therapeutic effects of astragalin (25, 50 or 75 mg/kg) on LPS- induced endotoxemia by dosing orally astragalin 1 hour before LPS challenge. Using an experimental model of LPS-induced acute lung injury (ALI), we examined the effect of astragalin in resolving lung injury. The investigations revealed that pretreatment with astragalin can improve survival during lethal endotoxemia and attenuate inflammatory responses in a murine model of lipopolysaccharide-induced acute lung injury. The mechanisms by which Astragalin exerts its anti-inflammatory effect are correlated with inhibition of tumor necrosis factor-α (TNF-α), interleukin-1 (IL-1), and interleukin-6 (IL-6) production via inactivation of NF-κB.
authors.affiliation: Department of Veterinary Pharmacology, College of Animal Science and Veterinary Medicine, Jilin University, Changchun, Jilin 130062, People's Republic of China.
authors.forename: Lanan Wassy
authors.initials: LW
authors.lastname: Soromou
authors.forename: Na
authors.initials: N
authors.lastname: Chen
authors.forename: Lanxiang
authors.initials: L
authors.lastname: Jiang
authors.forename: Meixia
authors.initials: M
authors.lastname: Huo
authors.forename: Miaomiao
authors.initials: M
authors.lastname: Wei
authors.forename: Xiao
authors.initials: X
authors.lastname: Chu
authors.forename: Faya Martin
authors.initials: FM
authors.lastname: Millimouno
authors.forename: Haihua
authors.initials: H
authors.lastname: Feng
authors.forename: Youssouf
authors.initials: Y
authors.lastname: Sidime
authors.forename: Xuming
authors.initials: X
authors.lastname: Deng
mesh_terms: D055371:Acute Lung Injury / Q000188:drug therapy* / Q000276:immunology; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000627:therapeutic use*; D004195:Disease Models, Animal; D015536:Down-Regulation; D019446:Endotoxemia / Q000188:drug therapy* / Q000276:immunology; D044949:Kaempferols / Q000737:chemistry / Q000627:therapeutic use*; D008070:Lipopolysaccharides / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000037:antagonists & inhibitors*; D018410:Pneumonia, Bacterial / Q000188:drug therapy*; D015398:Signal Transduction
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D044949:Kaempferols; D008070:Lipopolysaccharides; D016328:NF-kappa B; C001579:astragalin