title: Development and characterization of a monoclonal antibody against porcine CD205.
abstract: The aim of this work was to develop mAbs against porcine CD205 and to conduct a comparative analysis of the CD205 protein expression on lymphoid tissues, monocyte-derived dendritic cells (DCs) and DCs isolated from the porcine skin. To conduct this study, we generated a monoclonal antibody, designated 1.F6F6, against the C-type lectin-like domain-5 of the porcine CD205 and showed that it recognizes a protein band of ∼200 kDa by Western Blot analysis in mesenteric lymph nodes cells. Flow cytometric analysis showed that the mAb 1.F6F6 recognized 28.5%, 28.1% and 34.1% of cells from tonsil, inguinal and mesenteric lymph nodes, respectively, and 6% of cells from thymus. Analysis of monocyte-derived DCs showed that approximately 20% were positive and activation of the cells with LPS increased the positive population to 36%. Analysis of DCs isolated from the porcine skin showed that approximately 70% of the cell population expressed the CD205 receptor. The development of a monoclonal antibody capable of recognizing the CD205 receptor in swine opens up possibilities of applying new strategies for enhancing vaccine efficacy by using the anti-CD205 antibody for DC antigen-targeting to enhance priming of immune responses.
authors.affiliation: Laboratorio de Inmunología, Centro de Investigación en Alimentación y Desarrollo, A.C. Hermosillo, Sonora, México.
authors.forename: Lilian
authors.initials: L
authors.lastname: Flores-Mendoza
authors.forename: Carlos
authors.initials: C
authors.lastname: Velazquez
authors.forename: Jocelyn
authors.initials: J
authors.lastname: Bray
authors.forename: Leo
authors.initials: L
authors.lastname: Njongmeta
authors.forename: Waithaka
authors.initials: W
authors.lastname: Mwangi
authors.forename: Jesús
authors.initials: J
authors.lastname: Hernández
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D015703:Antigens, CD / Q000276:immunology*; D002460:Cell Line; D003713:Dendritic Cells / Q000276:immunology; D037181:Lectins, C-Type / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D015778:Minor Histocompatibility Antigens; D011956:Receptors, Cell Surface / Q000276:immunology*; D013552:Swine
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; C093569:DEC-205 receptor; D037181:Lectins, C-Type; D015778:Minor Histocompatibility Antigens; D011956:Receptors, Cell Surface