title: Bilirubin oxidase from Magnaporthe oryzae: an attractive new enzyme for biotechnological applications.
abstract: A novel bilirubin oxidase (BOD), from the rice blast fungus Magnaporthe oryzae, has been identified and isolated. The 64-kDa protein containing four coppers was successfully overexpressed in Pichia pastoris and purified to homogeneity in one step. Protein yield is more than 100 mg for 2 L culture, twice that of Myrothecium verrucaria. The k(cat)/K(m) ratio for conjugated bilirubin (1,513 mM⁻¹ s⁻¹) is higher than that obtained for the BOD from M. verrucaria expressed in native fungus (980 mM⁻¹ s⁻¹), with the lowest K(m) measured for any BOD highly desirable for detection of bilirubin in medical samples. In addition, this protein exhibits a half-life for deactivation >300 min at 37 °C, high stability at pH 7, and high tolerance towards urea, making it an ideal candidate for the elaboration of biofuel cells, powering implantable medical devices. Finally, this new BOD is efficient in decolorizing textile dyes such as Remazol brilliant Blue R, making it useful for environmentally friendly industrial applications.
authors.affiliation: CNRS, CRPP, UPR 8641, Université Bordeaux, 33600 Pessac, France.
authors.forename: Fabien
authors.initials: F
authors.lastname: Durand
authors.forename: Sébastien
authors.initials: S
authors.lastname: Gounel
authors.forename: Christian H
authors.initials: CH
authors.lastname: Kjaergaard
authors.forename: Edward I
authors.initials: EI
authors.lastname: Solomon
authors.forename: Nicolas
authors.initials: N
authors.lastname: Mano
mesh_terms: D001709:Biotechnology; D004795:Enzyme Stability; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D015870:Gene Expression; D007700:Kinetics; D020082:Magnaporthe / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D044925:Oxidoreductases Acting on CH-CH Group Donors; C042401:bilirubin oxidase