title: Identification and localisation of the NB-LRR gene family within the potato genome.
abstract: BACKGROUND
  The potato genome sequence derived from the Solanum tuberosum Group Phureja clone DM1-3 516 R44 provides unparalleled insight into the genome composition and organisation of this important crop. A key class of genes that comprises the vast majority of plant resistance (R) genes contains a nucleotide-binding and leucine-rich repeat domain, and is collectively known as NB-LRRs.
  
  
  RESULTS
  As part of an effort to accelerate the process of functional R gene isolation, we performed an amino acid motif based search of the annotated potato genome and identified 438 NB-LRR type genes among the ~39,000 potato gene models. Of the predicted genes, 77 contain an N-terminal toll/interleukin 1 receptor (TIR)-like domain, and 107 of the remaining 361 non-TIR genes contain an N-terminal coiled-coil (CC) domain. Physical map positions were established for 370 predicted NB-LRR genes across all 12 potato chromosomes. The majority of NB-LRRs are physically organised within 63 identified clusters, of which 50 are homogeneous in that they contain NB-LRRs derived from a recent common ancestor.
  
  
  CONCLUSIONS
  By establishing the phylogenetic and positional relationship of potato NB-LRRs, our analysis offers significant insight into the evolution of potato R genes. Furthermore, the data provide a blueprint for future efforts to identify and more rapidly clone functional NB-LRR genes from Solanum species.
authors.affiliation: Cell and Molecular Sciences, The James Hutton Institute (JHI), Dundee, DD2 5DA, UK.
authors.forename: Florian
authors.initials: F
authors.lastname: Jupe
authors.forename: Leighton
authors.initials: L
authors.lastname: Pritchard
authors.forename: Graham J
authors.initials: GJ
authors.lastname: Etherington
authors.forename: Katrin
authors.initials: K
authors.lastname: Mackenzie
authors.forename: Peter J A
authors.initials: PJ
authors.lastname: Cock
authors.forename: Frank
authors.initials: F
authors.lastname: Wright
authors.forename: Sanjeev Kumar
authors.initials: SK
authors.lastname: Sharma
authors.forename: Dan
authors.initials: D
authors.lastname: Bolser
authors.forename: Glenn J
authors.initials: GJ
authors.lastname: Bryan
authors.forename: Jonathan D G
authors.initials: JD
authors.lastname: Jones
authors.forename: Ingo
authors.initials: I
authors.lastname: Hein
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D002874:Chromosome Mapping; D016000:Cluster Analysis; D018745:Genome, Plant; D007930:Leucine / Q000737:chemistry; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000032:analysis / Q000235:genetics*; D011198:Solanum tuberosum / Q000145:classification / Q000235:genetics*
chemical_list: D010940:Plant Proteins; D007930:Leucine