title: Surface-modified silicone foils for intraocular implantation.
abstract: BACKGROUND
  Implantation of silicone materials like iris diaphragms into the eye can be complicated by cell migration and attachment. We studied polydimethylsiloxane (PDMS) foils coated with isocyanate terminated, star-shaped poly(ethylene glycol-stat-propylene glycol) (NCO-sP(EO-stat-PO)) equipped with heparin towards the inhibition of cell attachment without influencing cell viability.
  
  
  METHODS
  Mouse fibroblasts L929 were cultured and seeded onto sterilized pieces of either uncoated NCO-sP(EO-stat-PO) or heparin-NCO-sP(EO-stat-PO) loaded foils. Polyvinylchloride (PVC) foils served as the positive control and biomembranes as the negative control. The cultured cells were examined after 24 h for cell viability and adhesion by fluorescence microscopy; morphological cell changes were documented after hemalaun staining. Cell density was measured and quantification of cell proliferation was assessed by a BrdU test; quantification of cell activity was analyzed by a WST-1 test.
  
  
  RESULTS
  The fibroblasts' cell viability was excellent on all tested foils except the toxic PVC foil. NCO-sP(EO-stat-PO) coating provided significantly reduced cell activity. On heparin-loaded coatings, cells were viable and less dense but showed almost the same cell proliferation and cell activity as on the negative control. NCO-sP(EO-stat-PO) coated, heparin loaded foils proved high biocompatibility and reduced cell adhesion.
  
  
  CONCLUSIONS
  Both NCO-sP(EO-stat-PO)-coated foils with and without heparin seemed to be a viable implantation material for less cell migration, attachment, and reduced implant complications. Conclusive we give a recommendation for further studies on the intraocular implantation in particular for the NCO-sP(EO-stat-PO)-coated foils.
authors.affiliation: Department of Ophthalmology, RWTH Aachen University, Pauwelsstraße 30, 52074 Aachen, Germany. sarah__fischer@web.de
authors.forename: Sarah
authors.initials: S
authors.lastname: Fischer
authors.forename: Dörthe
authors.initials: D
authors.lastname: Carstesen
authors.forename: Doris
authors.initials: D
authors.lastname: Klee
authors.forename: Peter
authors.initials: P
authors.lastname: Walter
authors.forename: Andreas W A
authors.initials: AW
authors.lastname: Weinberger
mesh_terms: D000818:Animals; D017209:Apoptosis; D001187:Artificial Organs; D002448:Cell Adhesion / Q000502:physiology; D049109:Cell Proliferation; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible; D004129:Dimethylpolysiloxanes; D005347:Fibroblasts / Q000166:cytology*; D006493:Heparin; D007498:Iris; D008422:Materials Testing; D051379:Mice; D011092:Polyethylene Glycols; D019946:Propylene Glycol
chemical_list: D020099:Coated Materials, Biocompatible; D004129:Dimethylpolysiloxanes; D011092:Polyethylene Glycols; C013830:baysilon; D019946:Propylene Glycol; D006493:Heparin