title: Glycomics for drug discovery: metabolic perturbation in androgen-independent prostate cancer cells induced by unnatural hexosamine mimics.
abstract: Inhibited: N-acetylglucosamine (GlcNAc) derivatives with a fluorine atom at the C4 position (2-4) were synthesized, and their ability to inhibit cancer-cell growth was investigated. The administration of these 4F-GlcNAc derivatives to cells led to the unnatural sugar nucleotide 1. Furthermore, N-glycan profiles of cells were determined by using a glycoblotting-based enrichment analysis, which is suitable for high-throughput screenings for drug discovery.
authors.affiliation: Graduate School of Advanced Life Science, Hokkaido University, Kita-ku, Sapporo, Japan. shin@sci.hokudai.acjp
authors.forename: Shin-Ichiro
authors.initials: S
authors.lastname: Nishimura
authors.forename: Megumi
authors.initials: M
authors.lastname: Hato
authors.forename: Satoshi
authors.initials: S
authors.lastname: Hyugaji
authors.forename: Fei
authors.initials: F
authors.lastname: Feng
authors.forename: Maho
authors.initials: M
authors.lastname: Amano
mesh_terms: D000117:Acetylglucosamine / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D000728:Androgens / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D002240:Carbohydrate Sequence; D045744:Cell Line, Tumor; D004353:Drug Evaluation, Preclinical; D005461:Fluorine / Q000737:chemistry; D054794:Glycomics; D006031:Glycosylation; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D011471:Prostatic Neoplasms / Q000378:metabolism / Q000473:pathology
chemical_list: D000728:Androgens; D005461:Fluorine; D000117:Acetylglucosamine