title: Identifying FGA peptides as nasopharyngeal carcinoma-associated biomarkers by magnetic beads.
abstract: Early diagnosis and treatment is known to improve prognosis for nasopharyngeal carcinoma (NPC). The study determined the specific peptide profiles by comparing the serum differences between NPC patients and healthy controls, and provided the basis for the diagnostic model and identification of specific biomarkers of NPC. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) can be used to detect the molecular mass of peptides. Mass spectra of peptides were generated after extracting and purification of 40 NPC samples in the training set, 21 in the single center validation set and 99 in the multicenter validation set using weak cationic-exchanger magnetic beads. The spectra were analyzed statistically using FlexAnalysis™ and ClinProt™ bioinformatics software. The four most significant peaks were selected out to train a genetic algorithm model to diagnose NPC. The diagnostic sensitivity and specificity were 100% and 100% in the training set, 90.5% and 88.9% in the single center validation set, 91.9% and 83.3% in the multicenter validation set, and the false positive rate (FPR) and false negative rate (FNR) were obviously lower in the NPC group (FPR, 16.7%; FNR, 8.1%) than in the other cancer group (FPR, 39%; FNR, 61%), respectively. So, the diagnostic model including four peptides can be suitable for NPC but not for other cancers. FGA peptide fragments identified may serve as tumor-associated biomarkers for NPC.
authors.affiliation: Department of Radiation Oncology, Cancer Center, Sun Yat-Sen University, Guangzhou, Guangdong, People's Republic of China.
authors.forename: Ya-Lan
authors.initials: YL
authors.lastname: Tao
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Jin
authors.initials: J
authors.lastname: Gao
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Liu
authors.forename: Zi-Wei
authors.initials: ZW
authors.lastname: Tu
authors.forename: Guo
authors.initials: G
authors.lastname: Li
authors.forename: Bing-Qing
authors.initials: BQ
authors.lastname: Xu
authors.forename: Dao-Li
authors.initials: DL
authors.lastname: Niu
authors.forename: Chang-Bin
authors.initials: CB
authors.lastname: Jiang
authors.forename: Wei
authors.initials: W
authors.lastname: Yi
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Wang
authors.forename: Zhi-Bin
authors.initials: ZB
authors.lastname: Cheng
authors.forename: Qiao-Dan
authors.initials: QD
authors.lastname: Liu
authors.forename: Li
authors.initials: L
authors.lastname: Bai
authors.forename: Chun
authors.initials: C
authors.lastname: Zhang
authors.forename: Jing-Yu
authors.initials: JY
authors.lastname: Zhang
authors.forename: Mu-Sheng
authors.initials: MS
authors.lastname: Zeng
authors.forename: Yun-Fei
authors.initials: YF
authors.lastname: Xia
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D014408:Biomarkers, Tumor / Q000097:blood*; D002277:Carcinoma; D005260:Female; D005340:Fibrinogen / Q000032:analysis*; D006801:Humans; D060328:Magnetic Phenomena; D058185:Magnetite Nanoparticles; D008297:Male; D008875:Middle Aged; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000175:diagnosis*; D010455:Peptides / Q000097:blood*; D040901:Proteomics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D014408:Biomarkers, Tumor; D058185:Magnetite Nanoparticles; D010455:Peptides; D005340:Fibrinogen