title: Verrucosispora wenchangensis sp. nov., isolated from mangrove soil.
abstract: An actinomycete strain 234402(T) was isolated from a mangrove soil sample collected in Wenchang, China. Phylogenetic analysis of the 16S rRNA gene sequence of strain 234402(T) indicated that the highest similarity was to Verrucosispora sediminis MS426(T) (99.25%). The cell wall contained meso-diaminopimelic acid. The major menaquinones were MK-9(H(4)) and MK-9(H(6)), with MK-9(H(8)) as minor components. The characteristic whole-cell sugars were xylose, mannose and glucose. The phospholipid profile was found to comprise phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol mannoside and an unknown phospholipid. The DNA G+C content was 69.2 mol%. The results of physiological and biochemical tests and low DNA-DNA relatedness demonstrated strain 234402(T) could be readily distinguished from the closely related Verrucosispora species. On the basis of these phenotypic and genotypic data, strain 234402(T) represents a novel species of the genus Verrucosispora, for which the name Verrucosispora wenchangensis sp. nov. is proposed. The type strain is 234402(T) (=CCTCC AA 2011018(T)=DSM 45674(T)).
authors.affiliation: Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, People's Republic of China.
authors.forename: Qing-yi
authors.initials: QY
authors.lastname: Xie
authors.forename: Hai-peng
authors.initials: HP
authors.lastname: Lin
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.forename: Roselyn
authors.initials: R
authors.lastname: Brown
authors.forename: Michael
authors.initials: M
authors.lastname: Goodfellow
authors.forename: Zixin
authors.initials: Z
authors.lastname: Deng
authors.forename: Kui
authors.initials: K
authors.lastname: Hong
mesh_terms: D001482:Base Composition; D002241:Carbohydrates / Q000032:analysis; D002473:Cell Wall / Q000737:chemistry; D002681:China; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D008848:Micromonosporaceae / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000032:analysis
chemical_list: D002241:Carbohydrates; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid