title: Application of pyrosequencing for Salmonella enterica rapid identification.
abstract: Application of pyrosequencing of six Salmonella-specific genes as a rapid Salmonella identification method was tested. Primers for hns, hisJ and hilA had non-specific reactions with non-Salmonella strains. Primers for invA, iroB and fimY had specific PCR products and pyrosequences of Salmonella, suggesting that they were suitable for Salmonella rapid identification.
authors.affiliation: Microbiology Laboratory, Shijiazhuang Center for Disease Control and Prevention, 3 Likang Street, Shijiazhuang, Hebei, China.
authors.forename: Xiujuan
authors.initials: X
authors.lastname: Li
authors.forename: Fang
authors.initials: F
authors.lastname: Yang
authors.forename: Weili
authors.initials: W
authors.lastname: Gao
authors.forename: Hongmei
authors.initials: H
authors.lastname: Song
authors.forename: Huifang
authors.initials: H
authors.lastname: Tian
authors.forename: Baohong
authors.initials: B
authors.lastname: Xu
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001431:Bacteriological Techniques / Q000379:methods*; D017931:DNA Primers / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D019779:Salmonella enterica / Q000145:classification* / Q000235:genetics*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers