title: Kinetics of DNA methylation inheritance by the Dnmt1-including complexes during the cell cycle.
abstract: BACKGROUND
  The clonal transmission of lineage-specific DNA methylation patterns in a mammalian genome during the cellular division is a crucial biological process controlled by the DNA methyltransferase Dnmt1, mainly. To investigate possible dynamic mechanisms of DNA methylation inheritance during the cell cycle, we used a Proximity Ligation In Situ Assay (P-LISA) to analyze the kinetic of formation and DNA recruitment of Dnmt1-including complexes.
  
  
  RESULTS
  P-LISA, sequential chromatin immunoprecipitation and quantitative methylation specific PCR revealed that the Dnmt1/PCNA/UHRF1-including complexes are mainly formed and recruited on DNA during the S-phase of cell cycle, while the formation and the DNA recruitment of several Dnmt1/transcription factors-including complexes are not S-phase dependent but are G0/G1 and/or G2/M phases dependent.
  
  
  CONCLUSIONS
  Our data confirm that DNA methylation inheritance occurs in S-phase, and demonstrate that DNA methylation inheritance can also occur in G0/G1 and G2/M phases of the cell cycle.
authors.affiliation: Institut de Recherche Thérapeutique de l'Université de Nantes, INSERM U892, Centre de Recherche en Cancérologie Nantes-Angers, Equipe Apoptose et Progression Tumorale, 8 quai moncousu, BP7021, 44007 Nantes, France. pierre-francois.cartron@univ-nantes.fr.
authors.forename: Eric
authors.initials: E
authors.lastname: Hervouet
authors.forename: Arulraj
authors.initials: A
authors.lastname: Nadaradjane
authors.forename: Marine
authors.initials: M
authors.lastname: Gueguen
authors.forename: François M
authors.initials: FM
authors.lastname: Vallette
authors.forename: Pierre-François
authors.initials: PF
authors.lastname: Cartron