title: Contribution of IL-33-activated type II innate lymphoid cells to pulmonary eosinophilia in intestinal nematode-infected mice.
abstract: When animals are infected with helminthic parasites, resistant hosts show type II helper T immune responses to expel worms. Recently, natural helper (NH) cells or nuocytes, newly identified type II innate lymphoid cells, are shown to express ST2 (IL-33 receptor) and produce IL-5 and IL-13 when stimulated with IL-33. Here we show the relevant roles of endogenous IL-33 for Strongyloides venezuelensis infection-induced lung eosinophilic inflammation by using Il33(-/-) mice. Alveolar epithelial type II cells (ATII) express IL-33 in their nucleus. Infection with S. venezuelensis or intranasal administration of chitin increases in the number of ATII cells and the level of IL-33. S. venezuelensis infection induces pulmonary accumulation of NH cells, which, after being stimulated with IL-33, proliferate and produce IL-5 and IL-13. Furthermore, S. venezuelensis infected Rag2(-/-) mice increase the number of ATII cells, NH cells, and eosinophils and the expression of IL-33 in their lungs. Finally, IL-33-stimulated NH cells induce lung eosinophilic inflammation and might aid to expel infected worms in the lungs.
authors.affiliation: Department of Immunology and Medical Zoology, Hyogo College of Medicine, Nishinomiya 663-8501, Japan.
authors.forename: Koubun
authors.initials: K
authors.lastname: Yasuda
authors.forename: Taichiro
authors.initials: T
authors.lastname: Muto
authors.forename: Tatsukata
authors.initials: T
authors.lastname: Kawagoe
authors.forename: Makoto
authors.initials: M
authors.lastname: Matsumoto
authors.forename: Yuki
authors.initials: Y
authors.lastname: Sasaki
authors.forename: Kazufumi
authors.initials: K
authors.lastname: Matsushita
authors.forename: Yuko
authors.initials: Y
authors.lastname: Taki
authors.forename: Shizue
authors.initials: S
authors.lastname: Futatsugi-Yumikura
authors.forename: Hiroko
authors.initials: H
authors.lastname: Tsutsui
authors.forename: Ken J
authors.initials: KJ
authors.lastname: Ishii
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Yoshimoto
authors.forename: Shizuo
authors.initials: S
authors.lastname: Akira
authors.forename: Kenji
authors.initials: K
authors.lastname: Nakanishi
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D004268:DNA-Binding Proteins / Q000172:deficiency / Q000235:genetics; D018793:Interleukin-13 / Q000096:biosynthesis; D000067596:Interleukin-33; D015848:Interleukin-5 / Q000096:biosynthesis; D007378:Interleukins / Q000096:biosynthesis / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D007411:Intestinal Diseases, Parasitic / Q000150:complications* / Q000276:immunology / Q000473:pathology; D007814:Larva; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009559:Nippostrongylus / Q000254:growth & development / Q000276:immunology; D011650:Pulmonary Alveoli / Q000276:immunology / Q000473:pathology; D011657:Pulmonary Eosinophilia / Q000209:etiology* / Q000276:immunology; D017206:Strongylida Infections / Q000150:complications / Q000276:immunology / Q000473:pathology; D013321:Strongyloides / Q000254:growth & development / Q000276:immunology; D013322:Strongyloidiasis / Q000150:complications* / Q000276:immunology / Q000473:pathology
chemical_list: D004268:DNA-Binding Proteins; C506535:Il33 protein, mouse; D018793:Interleukin-13; D000067596:Interleukin-33; D015848:Interleukin-5; D007378:Interleukins; C499308:Rag2 protein, mouse