title: In vitro activities of antifungal agents alone and in combination against fluconazole-susceptible and -resistant strains of Candida dubliniensis.
abstract: In the present study we used two groups of Candida dubliniensis strains: one containing fluconazole-susceptible clinical isolates and another containing fluconazole-resistant laboratory derivative from the former to examine the changes on susceptibility accompanying the development of resistance to fluconazole. Our findings confirmed the ability of C. dubliniensis isolates to become resistant to fluconazole and indicated that this resistance was crossed with ketoconazole, itraconazole, ravuconazole and terbinafine. We also tested combinations of terbinafine, amphotericin B, itraconazole and voriconazole against both groups of isolates in a checkerboard assay. Surprisingly, most combinations evidenced indifferent interactions, and the best synergism appeared when terbinafine and itraconazole were combined against the fluconazole-resistant group.
authors.affiliation: Postgraduate Course in Pharmaceutical Sciences, Department of Microbiology and Parasitology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Liliane Alves
authors.initials: LA
authors.lastname: Scheid
authors.forename: Débora Alves Nunes
authors.initials: DA
authors.lastname: Mario
authors.forename: Thaís Felli
authors.initials: TF
authors.lastname: Kubiça
authors.forename: Janio Morais
authors.initials: JM
authors.lastname: Santurio
authors.forename: Sydney Hartz
authors.initials: SH
authors.lastname: Alves
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000145:classification / Q000187:drug effects*; D025141:Drug Resistance, Fungal / Q000187:drug effects*; D004357:Drug Synergism; D004359:Drug Therapy, Combination; D015725:Fluconazole / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests / Q000379:methods
chemical_list: D000935:Antifungal Agents; D015725:Fluconazole