title: Wavelet analysis for single molecule localization microscopy.
abstract: Localization of single molecules in microscopy images is a key step in quantitative single particle data analysis. Among them, single molecule based super-resolution optical microscopy techniques require high localization accuracy as well as computation of large data sets in the order of 10(5) single molecule detections to reconstruct a single image. We hereby present an algorithm based on image wavelet segmentation and single particle centroid determination, and compare its performance with the commonly used gaussian fitting of the point spread function. We performed realistic simulations at different signal-to-noise ratios and particle densities and show that the calculation time using the wavelet approach can be more than one order of magnitude faster than that of gaussian fitting without a significant degradation of the localization accuracy, from 1 nm to 4 nm in our range of study. We propose a simulation-based estimate of the resolution of an experimental single molecule acquisition.
authors.affiliation: Laboratoire Kastler Brossel, CNRS UMR 8552, Département de Physique et Institut de Biologie de l’Ecole Normale Supérieure, Université Pierre et Marie Curie-Paris 6, 46 rue d’Ulm 75005 Paris, France.
authors.forename: I
authors.initials: I
authors.lastname: Izeddin
authors.forename: J
authors.initials: J
authors.lastname: Boulanger
authors.forename: V
authors.initials: V
authors.lastname: Racine
authors.forename: C G
authors.initials: CG
authors.lastname: Specht
authors.forename: A
authors.initials: A
authors.lastname: Kechkar
authors.forename: D
authors.initials: D
authors.lastname: Nair
authors.forename: A
authors.initials: A
authors.lastname: Triller
authors.forename: D
authors.initials: D
authors.lastname: Choquet
authors.forename: M
authors.initials: M
authors.lastname: Dahan
authors.forename: J B
authors.initials: JB
authors.lastname: Sibarita
mesh_terms: D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008853:Microscopy / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*; D053758:Nanoparticles / Q000648:ultrastructure*; D058067:Wavelet Analysis