title: Acetylenic acid analogues from the edible mushroom Chanterelle (Cantharellus cibarius) and their effects on the gene expression of peroxisome proliferator-activated receptor-gamma target genes.
abstract: A new acetylenic acid, (10E,14Z)-9-oxooctadeca-10,14-dien-12-ynoic acid (1), was isolated from the edible mushroom Chanterelle (Cantharellus cibarius), together with a known acetylenic acid, (10E,14Z)-9-hydroxyoctadeca-10,14-dien-12-ynoic acid (2) and their structures were determined through analysis of NMR and mass data. The new acetylenic acid (1) specifically activated peroxisome proliferator-activated receptor (PPAR)-γ with an EC(50) value of 1.88 μM as measured by a reporter gene assay. Expression of PPAR-γ target genes were significantly altered as well, supporting the hypothesis that compound 1 is a PPAR-γ potential agonist that regulates transcription of the PPAR-γ target genes.
authors.affiliation: Department of Biotechnology, Graduate School of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea.
authors.forename: Seong Su
authors.initials: SS
authors.lastname: Hong
authors.forename: Ji Hae
authors.initials: JH
authors.lastname: Lee
authors.forename: Wonsik
authors.initials: W
authors.lastname: Jeong
authors.forename: Nahyun
authors.initials: N
authors.lastname: Kim
authors.forename: Hui Zi
authors.initials: HZ
authors.lastname: Jin
authors.forename: Bang Yeon
authors.initials: BY
authors.lastname: Hwang
authors.forename: Hak-Ju
authors.initials: HJ
authors.lastname: Lee
authors.forename: Sung-Joon
authors.initials: SJ
authors.lastname: Lee
authors.forename: Dae Sik
authors.initials: DS
authors.lastname: Jang
authors.forename: Dongho
authors.initials: D
authors.lastname: Lee
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000480:Alkynes / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D016466:CHO Cells; D002839:Chromans / Q000494:pharmacology; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D005231:Fatty Acids, Unsaturated / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D015870:Gene Expression; D017930:Genes, Reporter; D056945:Hep G2 Cells; D006801:Humans; D007004:Hypoglycemic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D008156:Luciferases / Q000235:genetics; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D047495:PPAR gamma / Q000819:agonists* / Q000235:genetics / Q000378:metabolism; D045162:Thiazolidinediones / Q000494:pharmacology; D014162:Transfection; D000077288:Troglitazone
chemical_list: D000480:Alkynes; D000970:Antineoplastic Agents; D002839:Chromans; D005231:Fatty Acids, Unsaturated; D007004:Hypoglycemic Agents; D047495:PPAR gamma; D045162:Thiazolidinediones; C031203:2-hexadecynoic acid; D008156:Luciferases; D000077288:Troglitazone