title: Production of l-lactic acid from a green microalga, Hydrodictyon reticulum, by Lactobacillus paracasei LA104 isolated from the traditional Korean food, makgeolli.
abstract: The freshwater microalga, Hydrodictyon reticulum, that contained 47.5% reducing sugars including 35% glucose was used as substrate for the production of l-lactic acid (LA) by LA-producing bacteria. Lactobacillus paracasei LA104 was selected for fermentation in a 5-l fermentor since it was able to grow at pH 3, 60g LA/l, 200g glucose/l, 125g NaCl/l, and 45°C and produced over 97.3% optically pure l-lactic acid with glucose as a substrate. Simultaneous saccharification and cofermentation from H. reticulum to l-LA using LA104 was investigated in a jar fermentor. The yield reached 46g/100g H. reticulum dry material, with a final concentration of 37.11g/l and a productivity of 1.03g/l/h. This is the first report of the production of l-LA from a microalga, and H. reticulum could be a potential feedstock for large-scale production of l-LA by LA104.
authors.affiliation: University of Science and Technology, Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology, Daejeon 305-600, Republic of Korea.
authors.forename: Cuong Mai
authors.initials: CM
authors.lastname: Nguyen
authors.forename: Jin-Seog
authors.initials: JS
authors.lastname: Kim
authors.forename: Hyun Jin
authors.initials: HJ
authors.lastname: Hwang
authors.forename: Myung Soo
authors.initials: MS
authors.lastname: Park
authors.forename: Gyung Ja
authors.initials: GJ
authors.lastname: Choi
authors.forename: Yong Ho
authors.initials: YH
authors.lastname: Choi
authors.forename: Kyoung Soo
authors.initials: KS
authors.lastname: Jang
authors.forename: Jin-Cheol
authors.initials: JC
authors.lastname: Kim
mesh_terms: D005285:Fermentation; D005502:Food; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000378:metabolism*; D007778:Lactobacillus / Q000302:isolation & purification / Q000378:metabolism*; D058086:Microalgae / Q000378:metabolism*; D056910:Republic of Korea
chemical_list: D019344:Lactic Acid