title: Detection of anammox activity and 16S rRNA genes in ravine paddy field soil.
abstract: An anammox assay involving a ¹⁵N tracer and gas chromatography-mass spectrometry revealed that the potential anammox activity accounted for 1 to 5% of total N₂ production in a ravine paddy field, Japan. Among four 4-cm-deep layers, the top layer showed the highest activity. Clone libraries showed that the DNA in the top layer contained sequences related to those of Candidatus 'Brocadia fulgida', Ca. 'B. anammoxidans', and Ca. 'Kuenenia stuttgartiensis'. These results suggest that a specific population of anammox bacteria was present in paddy soils, although a small part of dinitrogen gas was emitted from the soil via anammox.
authors.affiliation: Institute for Global Change Adaptation Science, Ibaraki University, Mito, Ibaraki 310-8512, Japan. ysato@tobunken.go.jp
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Sato
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ohta
authors.forename: Takao
authors.initials: T
authors.lastname: Yamagishi
authors.forename: Yong
authors.initials: Y
authors.lastname: Guo
authors.forename: Tomoyasu
authors.initials: T
authors.lastname: Nishizawa
authors.forename: M Habibur
authors.initials: MH
authors.lastname: Rahman
authors.forename: Hisao
authors.initials: H
authors.lastname: Kuroda
authors.forename: Task
authors.initials: T
authors.lastname: Kato
authors.forename: Masanori
authors.initials: M
authors.lastname: Saito
authors.forename: Ikuo
authors.initials: I
authors.lastname: Yoshinaga
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Inubushi
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Suwa
mesh_terms: D000641:Ammonia / Q000378:metabolism*; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000378:metabolism*; D044822:Biodiversity; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D008401:Gas Chromatography-Mass Spectrometry; D007553:Isotope Labeling; D007564:Japan; D054892:Metagenome; D009587:Nitrogen Isotopes; D010084:Oxidation-Reduction; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D009587:Nitrogen Isotopes; D012336:RNA, Ribosomal, 16S; D000641:Ammonia