title: Paucity of HIV DNA methylation in latently infected, resting CD4+ T cells from infected individuals receiving antiretroviral therapy.
abstract: Maintenance of HIV latency in vitro has been linked to methylation of HIV DNA. However, examinations of the degree of methylation of HIV DNA in the latently infected, resting CD4(+) T cells of infected individuals receiving antiretroviral therapy have been limited. Here, we show that methylation of the HIV 5' long terminal repeat (LTR) in the latent viral reservoir of HIV-infected aviremic individuals receiving therapy is rare, suggesting that other mechanisms are likely involved in the persistence of viral latency.
authors.affiliation: National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: Jana
authors.initials: J
authors.lastname: Blazkova
authors.forename: Danielle
authors.initials: D
authors.lastname: Murray
authors.forename: J Shawn
authors.initials: JS
authors.lastname: Justement
authors.forename: Emily K
authors.initials: EK
authors.lastname: Funk
authors.forename: Amy K
authors.initials: AK
authors.lastname: Nelson
authors.forename: Susan
authors.initials: S
authors.lastname: Moir
authors.forename: Tae-Wook
authors.initials: TW
authors.lastname: Chun
authors.forename: Anthony S
authors.initials: AS
authors.lastname: Fauci
mesh_terms: D023241:Antiretroviral Therapy, Highly Active; D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes / Q000821:virology*; D018899:CpG Islands; D019175:DNA Methylation; D004742:Enhancer Elements, Genetic; D023061:Gene Order; D015752:Genes, env; D006678:HIV / Q000235:genetics*; D015658:HIV Infections / Q000188:drug therapy / Q000821:virology*; D016325:HIV Long Terminal Repeat; D006801:Humans; D011401:Promoter Regions, Genetic; D011533:Proviruses / Q000235:genetics*; D019562:Viral Load; D017735:Virus Latency