title: Cytokine-based high log-scale expansion of functional human dendritic cells from cord-blood CD34-positive cells.
abstract: Dendritic cells (DCs) play a crucial role in maintaining the immune system. Though DC-based cancer immunotherapy has been suggested as a potential treatment for various kinds of malignancies, its clinical efficacies are still insufficient in many human trials. Issues that limit the clinical efficacy of DC-based immunotherapy, as well as the difficulty of the industrial production of DCs, are largely due to the limited number of autologous DCs available from each patient. We here established a possible breakthrough, a simple cytokine-based culture method to expand the log-scale order of functional human DCs. Floating cultivation of cord-blood CD34(+) cells under an optimized cytokine cocktail led these progenitor cells to stable log-scale proliferation and to DC differentiation. The expanded DCs had typical features of conventional myeloid DCs in vitro. Therefore, the concept of DC expansion should contribute significantly to the progress of DC immunotherapy.
authors.affiliation: Department of Urology, Chiba University Graduate School ofMedicine, Chiba 260-8670, Japan. rkfraile@med.kyushu-u.ac.jp
authors.forename: Yui
authors.initials: Y
authors.lastname: Harada
authors.forename: Yae
authors.initials: Y
authors.lastname: Okada-Nakanishi
authors.forename: Yasuji
authors.initials: Y
authors.lastname: Ueda
authors.forename: Shunichi
authors.initials: S
authors.lastname: Tsujitani
authors.forename: Satoru
authors.initials: S
authors.lastname: Saito
authors.forename: Terumi
authors.initials: T
authors.lastname: Fuji-Ogawa
authors.forename: Akihiro
authors.initials: A
authors.lastname: Iida
authors.forename: Mamoru
authors.initials: M
authors.lastname: Hasegawa
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Ichikawa
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Yonemitsu
mesh_terms: D018952:Antigens, CD34 / Q000378:metabolism*; D039521:CD11c Antigen / Q000378:metabolism; D015820:Cadherins / Q000378:metabolism; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism*; D003713:Dendritic Cells / Q000166:cytology*; D005312:Fetal Blood / Q000166:cytology; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D006801:Humans; D007167:Immunotherapy; D013601:T-Lymphocytes / Q000378:metabolism
chemical_list: D018952:Antigens, CD34; D039521:CD11c Antigen; D015820:Cadherins; D016207:Cytokines