title: Gene and protein expression of progesterone receptor isoforms A and B, p53 and p21 in myometrium and uterine leiomyoma.
abstract: OBJECTIVE
  To assess gene and protein expression of progesterone receptor isoforms A and B, cell cycle regulators p53 and p21 in leiomyoma and myometrium.
  
  
  METHODS
  Samples were collected from 14 patients in reproductive age who underwent abdominal hysterectomy. Gene expression of PRA, PRB, p53 and p21 was analyzed by real-time PCR. Protein expression was assessed by Western blots.
  
  
  RESULTS
  There was no change in gene and protein expression of PRA and PRB in both tissues. The ratio between isoforms (PRA:PRB) was not different between tissues and showed a strong correlation (r = 0.767, P = 0.004). The analysis of gene expression and protein showed increased levels of mRNA and protein p53 in leiomyoma compared to myometrium (P = 0.030 and P = 0.002, respectively). The same increase was observed in p21 mRNA levels (P = 0.016) and protein p21 levels (P = 0.026) in samples of uterine leiomyoma.
  
  
  CONCLUSIONS
  PRA:PRB ratio is similar in normal myometrium and leiomyomas. p53 and p21 mRNA and protein levels are increased in leiomyomas.
authors.affiliation: Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Lora
authors.forename: Anelise Olmos
authors.initials: AO
authors.lastname: Grings
authors.forename: Edison
authors.initials: E
authors.lastname: Capp
authors.forename: Helena
authors.initials: H
authors.lastname: von Eye Corleta
authors.forename: Ilma Simoni
authors.initials: IS
authors.lastname: Brum
mesh_terms: D003430:Cross-Sectional Studies; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D006801:Humans; D007889:Leiomyoma / Q000235:genetics* / Q000378:metabolism*; D009215:Myometrium / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011980:Receptors, Progesterone / Q000235:genetics / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism; D014594:Uterine Neoplasms / Q000235:genetics* / Q000378:metabolism*
chemical_list: D050759:Cyclin-Dependent Kinase Inhibitor p21; D012333:RNA, Messenger; D011980:Receptors, Progesterone; D016159:Tumor Suppressor Protein p53; C101118:progesterone receptor A; C097314:progesterone receptor B