title: [Study of proteomic profile of Danio rerio embryos using one-dimensional electrophoresis and mass spectrometry].
abstract: In the present study, a proteomic technology combining one-dimensional gel electrophoresis (1DE) with subsequent mass spectrometry (MALDI-TOF-PMF) has been successfully applied for revelation of changes in the protein profile of zebrafish (Danio rerio) 52 hpf embryos. Prior to 1DE separation of zebrafish embryonic proteins, the procedure for obtaining embryos homogenate was optimized by ultrasonic treatment. A total of 84 proteins, including 15 vitellogenins, were identified. It was shown that growing ofzebrafish embryos in the medium with doxorubicin (DOX) stimulated Caspase-3 induction and promoted the disappearance of cardiac troponins, both these findings being consistent with literature data on doxorubicin-induced cardiotoxicity. The 1DE-based proteomic mapping approach proposed herein enabled not only to identify proteins but also to register those changes in embryos' proteomic profile that were caused by doxorubicin.
authors.forename: Iu S
authors.initials: IuS
authors.lastname: Kisrieva
authors.forename: N A
authors.initials: NA
authors.lastname: Petushkova
authors.forename: A S
authors.initials: AS
authors.lastname: Chernobrovkin
authors.forename: O V
authors.initials: OV
authors.lastname: Larina
authors.forename: O P
authors.initials: OP
authors.lastname: Trifonova
authors.forename: N F
authors.initials: NF
authors.lastname: Samenkova
authors.forename: G P
authors.initials: GP
authors.lastname: Kuznetsova
authors.forename: I I
authors.initials: II
authors.lastname: Karuzina
authors.forename: V N
authors.initials: VN
authors.lastname: Kashirtseva
authors.forename: N F
authors.initials: NF
authors.lastname: Beliaeva
authors.forename: A V
authors.initials: AV
authors.lastname: Lisitsa
mesh_terms: D000818:Animals; D004317:Doxorubicin / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015027:Zebrafish / Q000196:embryology* / Q000378:metabolism; D029961:Zebrafish Proteins / Q000378:metabolism*
chemical_list: D020543:Proteome; D029961:Zebrafish Proteins; D004317:Doxorubicin