title: CopA3 peptide from Copris tripartitus induces apoptosis in human leukemia cells via a caspase-independent pathway.
abstract: Our previous study demonstrated that CopA3, a disulfide dimer of the coprisin peptide analogue (LLCIALRKK), has antibacterial activity. In this study, we assessed whether CopA3 caused cellular toxicity in various mammalian cell lines. CopA3 selectively caused a marked decrease in cell viability in Jurkat T, U937, and AML-2 cells (human leukemia cells), but was not cytotoxic to Caki or Hela cells. Fragmentation of DNA, a marker of apoptosis, was also confirmed in the leukemia cell lines, but not in the other cells. CopA3-induced apoptosis in leukemia cells was mediated by apoptosis inducing factor (AIF), indicating induction of a caspase-independent signaling pathway.
authors.affiliation: Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Daejin University, Pocheon 487-711, South Korea.
authors.forename: Bo Ram
authors.initials: BR
authors.lastname: Kang
authors.forename: Ho
authors.initials: H
authors.lastname: Kim
authors.forename: Sung-Hee
authors.initials: SH
authors.lastname: Nam
authors.forename: Eun-Young
authors.initials: EY
authors.lastname: Yun
authors.forename: Seong-Ryul
authors.initials: SR
authors.lastname: Kim
authors.forename: Mi-Young
authors.initials: MY
authors.lastname: Ahn
authors.forename: Jong Soo
authors.initials: JS
authors.lastname: Chang
authors.forename: Jae Sam
authors.initials: JS
authors.lastname: Hwang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D051033:Apoptosis Inducing Factor / Q000378:metabolism; D020169:Caspases / Q000378:metabolism*; D045744:Cell Line, Tumor; D001517:Coleoptera / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D019476:Insect Proteins / Q000138:chemical synthesis / Q000627:therapeutic use / Q000633:toxicity*; D019169:Jurkat Cells; D007938:Leukemia / Q000188:drug therapy / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D051033:Apoptosis Inducing Factor; D019476:Insect Proteins; C570442:coprisin peptide, Copris tripartitus; D020169:Caspases