title: CD99-dependent expansion of myeloid-derived suppressor cells and attenuation of graft-versus-host disease.
abstract: CD99 is involved in many cellular events, such as the generation of Hodgkin and Reed-Sternberg cells, T cell costimulation, and leukocyte transendothelial migration. However, these studies have been limited to in vitro or in vivo experiments using CD99-deficient cell lines or anti-CD99 antibodies. In the present study, using CD99-deficient mice established by the exchangeable gene trap method, we investigated the physiologic function of murine CD99. In a B6 splenocytes → bm12 graft-versus-host disease model, wild-type cells were minimally lethal, whereas all mice that received CD99-deficient donor cells developed an early and more severe pathology. Graftversus-host disease in these mice was associated with insufficient expansion of myeloid-derived suppressor cells. This was confirmed by experiments illustrating that the injection of wild-type donor cells depleted of Mac-1(+) cells led to an almost identical disease course as the CD99-deficient donor system. Therefore, these results suggest that CD99 plays a crucial role in the attenuation of graft-versus-host disease by regulating the expansion of myeloid-derived suppressor cells.
authors.affiliation: Department of Pathology, Seoul National University College of Medicine, Seoul 110-799, Korea.
authors.forename: Hyo Jin
authors.initials: HJ
authors.lastname: Park
authors.forename: Dahye
authors.initials: D
authors.lastname: Byun
authors.forename: An Hi
authors.initials: AH
authors.lastname: Lee
authors.forename: Ju Hyun
authors.initials: JH
authors.lastname: Kim
authors.forename: Young Larn
authors.initials: YL
authors.lastname: Ban
authors.forename: Masatake
authors.initials: M
authors.lastname: Araki
authors.forename: Kimi
authors.initials: K
authors.lastname: Araki
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Yamamura
authors.forename: Inho
authors.initials: I
authors.lastname: Kim
authors.forename: Seong Hoe
authors.initials: SH
authors.lastname: Park
authors.forename: Kyeong Cheon
authors.initials: KC
authors.lastname: Jung
mesh_terms: D000071443:12E7 Antigen; D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D002465:Cell Movement; D049109:Cell Proliferation; D017690:Cell Transplantation / Q000009:adverse effects; D016207:Cytokines / Q000097:blood; D006086:Graft vs Host Disease / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D022423:Myeloid Cells / Q000378:metabolism / Q000502:physiology*; D009368:Neoplasm Transplantation / Q000473:pathology; D013154:Spleen / Q000276:immunology / Q000473:pathology
chemical_list: D000071443:12E7 Antigen; D015703:Antigens, CD; C522605:Cd99 protein, mouse; D016207:Cytokines