title: RPA facilitates telomerase activity at chromosome ends in budding and fission yeasts.
abstract: In Saccharomyces cerevisiae, the telomerase complex binds to chromosome ends and is activated in late S-phase through a process coupled to the progression of the replication fork. Here, we show that the single-stranded DNA-binding protein RPA (replication protein A) binds to the two daughter telomeres during telomere replication but only its binding to the leading-strand telomere depends on the Mre11/Rad50/Xrs2 (MRX) complex. We further demonstrate that RPA specifically co-precipitates with yKu, Cdc13 and telomerase. The interaction of RPA with telomerase appears to be mediated by both yKu and the telomerase subunit Est1. Moreover, a mutation in Rfa1 that affects both the interaction with yKu and telomerase reduces the dramatic increase in telomere length of a rif1Δ, rif2Δ double mutant. Finally, we show that the RPA/telomerase association and function are conserved in Schizosaccharomyces pombe. Our results indicate that in both yeasts, RPA directly facilitates telomerase activity at chromosome ends.
authors.affiliation: Marseille Cancer Research Center CRCM, U1068 Inserm, UMR7258 CNRS, Aix-Marseille Univ, Institut Paoli-Calmettes, Marseille, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Luciano
authors.forename: Stéphane
authors.initials: S
authors.lastname: Coulon
authors.forename: Virginie
authors.initials: V
authors.lastname: Faure
authors.forename: Yves
authors.initials: Y
authors.lastname: Corda
authors.forename: Julia
authors.initials: J
authors.lastname: Bos
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Brill
authors.forename: Eric
authors.initials: E
authors.lastname: Gilson
authors.forename: Marie-Noelle
authors.initials: MN
authors.lastname: Simon
authors.forename: Vincent
authors.initials: V
authors.lastname: Géli
mesh_terms: D002875:Chromosomes / Q000378:metabolism*; D050505:Mutant Proteins / Q000235:genetics / Q000378:metabolism; D020125:Mutation, Missense; D011485:Protein Binding; D025941:Protein Interaction Mapping; D051716:Replication Protein A / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000201:enzymology* / Q000254:growth & development; D019098:Telomerase / Q000378:metabolism*; D016615:Telomere / Q000378:metabolism*
chemical_list: D050505:Mutant Proteins; C082728:RFA1 protein, S cerevisiae; D051716:Replication Protein A; D029701:Saccharomyces cerevisiae Proteins; D019098:Telomerase