title: A graphene oxide-peptide fluorescence sensor for proteolytically active prostate-specific antigen.
abstract: This paper presents a novel sensor to detect proteolytically active prostate-specific antigen (PSA) by assembling a purpose-designed FITC-labeled peptide with graphene oxide (GO). The fluorescence of the dye-labeled peptide was quenched in the presence of GO. Reaction of the sensor with PSA cleaves the peptide, leading to the release of the dye moiety and a great increase in fluorescence intensity in a dose- and time-dependent manner, and PSA can be quantified accordingly. This approach is simple compared to existing methods since the GO-peptide-based sensor is easily assembled and detection can be achieved without the involvement of complicated procedures. Moreover, the applicability of the method has been demonstrated by detecting PSA in spiked urine samples.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Feng
authors.forename: Duan
authors.initials: D
authors.lastname: Feng
authors.forename: Wen
authors.initials: W
authors.lastname: Shi
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Li
authors.forename: Huimin
authors.initials: H
authors.lastname: Ma
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D005260:Female; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D008297:Male; D018625:Microscopy, Atomic Force; D010087:Oxides / Q000737:chemistry*; D010455:Peptides / Q000652:urine*; D017430:Prostate-Specific Antigen / Q000652:urine*; D059748:Proteolysis; D013050:Spectrometry, Fluorescence
chemical_list: D010087:Oxides; D010455:Peptides; D006108:Graphite; D017430:Prostate-Specific Antigen; D016650:Fluorescein-5-isothiocyanate