title: An SNP selection strategy identified IL-22 associating with susceptibility to tuberculosis in Chinese.
abstract: Recent studies showed that IL-22 plays a protective role in the host defense. However, the contribution of polymorphisms of the IL-22 gene to human TB susceptibility remains untested. We have designed a computational approach to select functional SNPs in the IL-22 gene and genotyped them in a two-stage case-control study in Chinese (479 cases and 358 controls). We found that rs2227473, an SNP in the promoter region of IL-22, is associated with TB susceptibility at both stages of our study. The SNP shows associations with p-values of 0.028 and 0.034 respectively, and a combined p-value of 0.0086, with odds ratio at 0.65 (95% confidence interval 0.45-0.90). We further validated the association with an independent cohort (413 cases and 241 controls in Chinese). Our functional studies showed that patients with A allele have significantly higher IL-22 expression than those without A allele under both non-specific and specific stimulations.
authors.affiliation: Shenzhen–Hong Kong Institute of Infectious Disease, Shenzhen Third People’s Hospital, Guangdong Medical College, Shenzhen, 518020, China.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Zhang
authors.forename: Xinchun
authors.initials: X
authors.lastname: Chen
authors.forename: Long
authors.initials: L
authors.lastname: Chan
authors.forename: Mingxia
authors.initials: M
authors.lastname: Zhang
authors.forename: Baohua
authors.initials: B
authors.lastname: Zhu
authors.forename: Lantian
authors.initials: L
authors.lastname: Wang
authors.forename: Xiuyun
authors.initials: X
authors.lastname: Zhu
authors.forename: Jieyun
authors.initials: J
authors.lastname: Zhang
authors.forename: Boping
authors.initials: B
authors.lastname: Zhou
authors.forename: Junwen
authors.initials: J
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002681:China / Q000453:epidemiology; D005260:Female; D020022:Genetic Predisposition to Disease / Q000453:epidemiology* / Q000235:genetics*; D006801:Humans; D007378:Interleukins / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D015995:Prevalence; D018570:Risk Assessment; D012641:Selection, Genetic / Q000235:genetics*; D014376:Tuberculosis / Q000453:epidemiology* / Q000235:genetics*; D055815:Young Adult; D000097043:Interleukin-22
chemical_list: D007378:Interleukins; D000097043:Interleukin-22