title: [Analysis of mRNA expression profiles of megakaryocytes from human cord blood CD34+ cells ex vivo expanded using Solexa sequencing].
abstract: OBJECTIVE
  To investigate the mRNA expression profiles of megakaryocytes (MKs) from human cord blood CD34+ cells ex vivo expanded using Solexa technique.
  
  
  METHODS
  CD34+ Cells were isolated using density gradient centrifugation and magnetic activated cell sorting. Cultures were stimulated with recombinant human thrombopoietin (100 ng/ml). After 12 days, the MKs fraction was separated from the non-MKs fraction using an anti-CD41 monoclonal antibody by immunomagnetic sorting. The mRNA expression of MKs and non-MKs was detected by Solexa sequencing.
  
  
  RESULTS
  We obtained 3 773 147 and 3 533 805 Tags from MKs and non-MKs, respectively. The amounts of unambiguous tags were 3 291 132 and 2 967 947 and those of distinct tags were 197 769 and 245 318. The expression of 1161 genes was up-regulated and that of 902 genes down-regulated. The expression of 2717 tags was up-regulated and that of 1519 tags down-regulated.
  
  
  CONCLUSIONS
  MKs and non-MKs have remarkably different mRNA expression profiles. The differential gene-encoded products may be involved in cellular development, adhesion, apoptosis metabolism, intra- and intercellular signal transduction, and immune response. Further studies on this topic may clarify the expression mechanism, signal transduction, and regulation mechanisms.
authors.affiliation: Blood Center of Zhejiang Province, Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou 310006, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Ji
authors.initials: J
authors.lastname: He
authors.forename: Fa-Ming
authors.initials: FM
authors.lastname: Zhu
authors.forename: Jin-Hui
authors.initials: JH
authors.lastname: Liu
authors.forename: Fei
authors.initials: F
authors.lastname: Qin
authors.forename: Shu
authors.initials: S
authors.lastname: Chen
authors.forename: Gang
authors.initials: G
authors.lastname: Xu
authors.forename: Xing-Jun
authors.initials: XJ
authors.lastname: Lü
authors.forename: Li-Xing
authors.initials: LX
authors.lastname: Yan
mesh_terms: D018952:Antigens, CD34; D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology*; D006801:Humans; D008533:Megakaryocytes / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D059467:Transcriptome
chemical_list: D018952:Antigens, CD34; D012333:RNA, Messenger