title: Autosomal monoallelic expression in the mouse.
abstract: BACKGROUND
  Random monoallelic expression defines an unusual class of genes displaying random choice for expression between the maternal and paternal alleles. Once established, the allele-specific expression pattern is stably maintained and mitotically inherited. Examples of random monoallelic genes include those found on the X-chromosome and a subset of autosomal genes, which have been most extensively studied in humans. Here, we report a genome-wide analysis of random monoallelic expression in the mouse. We used high density mouse genome polymorphism mapping arrays to assess allele-specific expression in clonal cell lines derived from heterozygous mouse strains.
  
  
  RESULTS
  Over 1,300 autosomal genes were assessed for allele-specific expression, and greater than 10% of them showed random monoallelic expression. When comparing mouse and human, the number of autosomal orthologs demonstrating random monoallelic expression in both organisms was greater than would be expected by chance. Random monoallelic expression on the mouse autosomes is broadly similar to that in human cells: it is widespread throughout the genome, lacks chromosome-wide coordination, and varies between cell types. However, for some mouse genes, there appears to be skewing, in some ways resembling skewed X-inactivation, wherein one allele is more frequently active.
  
  
  CONCLUSIONS
  These data suggest that autosomal random monoallelic expression was present at least as far back as the last common ancestor of rodents and primates. Random monoallelic expression can lead to phenotypic variation beyond the phenotypic variation dictated by genotypic variation. Thus, it is important to take into account random monoallelic expression when examining genotype-phenotype correlation.
authors.affiliation: Center for Human Genetic Research, Massachusetts General Hospital, Boston, MA 02114, USA.
authors.forename: Lillian M
authors.initials: LM
authors.lastname: Zwemer
authors.forename: Alexander
authors.initials: A
authors.lastname: Zak
authors.forename: Benjamin R
authors.initials: BR
authors.lastname: Thompson
authors.forename: Andrew
authors.initials: A
authors.lastname: Kirby
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Daly
authors.forename: Andrew
authors.initials: A
authors.lastname: Chess
authors.forename: Alexander A
authors.initials: AA
authors.lastname: Gimelbrant
mesh_terms: D000483:Alleles; D022981:Allelic Imbalance / Q000235:genetics*; D000818:Animals; D002875:Chromosomes / Q000235:genetics*; D015870:Gene Expression / Q000235:genetics*; D056726:Genetic Association Studies; D016678:Genome; D005838:Genotype; D006801:Humans; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D020641:Polymorphism, Single Nucleotide; D014960:X Chromosome / Q000235:genetics