title: An unusual case of multi-recurrent herpes zoster (Hz): a case report.
abstract: OBJECTIVE
  We report a case of multi-recurrent herpes zoster in a 53-year-old Caucasian woman treated repeatedly at the Faculty Hospital Hradec Kralove, Czech Republic over the years 2009 - 2011.
  
  
  METHODS
  Specific PCR methods targeting single nucleotide polymorphisms (SNPs) in open reading frames (ORF) 38, 54 and 62 were utilized to determine vaccine or wild type varicella-zoster (VZV) strains followed by SNPs analysis using two amplicons in ORF 22 and/or ORF 21/ORF 50. Additional genotyping in ORF 1, 6, 9 and 28 was subsequently performed due to the unusual results.
  
  
  RESULTS
  Three sets of clinical specimens from one patient (from hospital visits 2, 3 and 4) were analyzed and the presence of an unusual wild-type strain of VZV was discovered. The VZV strain isolated from the lesions bears a combination of markers characteristic both for Mosaic 2 (M2) and European 1 (E1) wild-type VZV strains.
  
  
  CONCLUSIONS
  This is the first report of atypical wild-type VZV strain circulating currently in Czech Republic.
authors.affiliation: University of Defence, Hradec Kralove, Czech Republic.
authors.forename: Vanda
authors.initials: V
authors.lastname: Bostikova
authors.forename: Miloslav
authors.initials: M
authors.lastname: Salavec
authors.forename: Jan
authors.initials: J
authors.lastname: Smetana
authors.forename: Roman
authors.initials: R
authors.lastname: Chlibek
authors.forename: Pavel
authors.initials: P
authors.lastname: Kosina
authors.forename: Petr
authors.initials: P
authors.lastname: Prasil
authors.forename: Stanislav
authors.initials: S
authors.lastname: Plisek
authors.forename: Miroslav
authors.initials: M
authors.lastname: Splino
authors.forename: Pavel
authors.initials: P
authors.lastname: Bostik
mesh_terms: D005260:Female; D016678:Genome; D006562:Herpes Zoster / Q000821:virology*; D014645:Herpesvirus 3, Human / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008875:Middle Aged; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D012008:Recurrence