title: Total polyphenols, antioxidant and antiproliferative activities of different extracts in mungbean seeds and sprouts.
abstract: The aim of this investigation was to evaluate the biological, alcohol dehydrogenase (ADH) and antiproliferative activities of different extracts of mungbean seeds and sprouts. All extracts from the sprouts showed higher contents of total phenolics (TP), total flavonoids (TF), and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity than from seeds. The highest DPPH and tyrosinase inhibition activities were registered in ethyl acetate (EtOAc) extract. ADH activity of methanol (MeOH), n-hexane (n-hexane) and n-butanol (n-BuOH) extracts from sprouts was significantly higher (P < 0.05) than from seeds. However, the highest ADH activity was found in water extract of seeds. According to 3-(4, 5-dimethylthiazol -2-yl)-2, 5-diphenyltetrazolium bromide) (MTT) assay, extracts from sprouts were more effective against Calu-6 (human pulmonary carcinoma) and SNU-601 (human gastric carcinoma) cells than from seeds. EtOAc extract showed the highest antiproliferative activity in both sprouts and seeds, followed by n-hexane extraction. During sprouting of mungbean, all the studied components significantly increased. In conclusion, the extracts of sprouts are more effective than from seeds and could be a potential source of antioxidants linked with health benefits.
authors.affiliation: Jeonnam Agricultural Research and Extension Services, Naju, 520-715, South Korea.
authors.forename: Dong-Kwan
authors.initials: DK
authors.lastname: Kim
authors.forename: Seok Cheol
authors.initials: SC
authors.lastname: Jeong
authors.forename: Shela
authors.initials: S
authors.lastname: Gorinstein
authors.forename: Sang-Uk
authors.initials: SU
authors.lastname: Chon
mesh_terms: D000426:Alcohol Dehydrogenase / Q000378:metabolism; D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis / Q000494:pharmacology; D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology; D045744:Cell Line, Tumor; D007887:Fabaceae / Q000737:chemistry* / Q000201:enzymology; D005419:Flavonoids / Q000032:analysis / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000032:analysis / Q000494:pharmacology; D006801:Humans; D020128:Inhibitory Concentration 50; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors; D010636:Phenols / Q000032:analysis / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D036226:Seedlings / Q000737:chemistry* / Q000201:enzymology; D012639:Seeds / Q000737:chemistry* / Q000201:enzymology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D005419:Flavonoids; D016166:Free Radical Scavengers; D010636:Phenols; D010936:Plant Extracts; D000426:Alcohol Dehydrogenase; D014442:Monophenol Monooxygenase