title: Detection of genetic variability in various isolates of cattle tick, Boophilus microplus from Tamil Nadu, India using PCR-RAPD analysis.
abstract: The genomic DNA from ten isolates of the cattle tick, Boophilus microplus collected in and around Chennai, India, was analyzed by random amplified polymorphic DNA (RAPD) using PCR. Selected five random primers were used for the study of genetic variability among different isolates of B. microplus. A high degree of genetic polymorphism with a different pattern of RAPD profiles for each tick isolate was detected with all these random primers. This variability was also confirmed by similarity coefficient values and dendrogram which were performed using mean RAPD profiles for all the primers between various isolates of ticks. The findings suggest the existence of a complex genotypic diversity of the tick B. microplus in an endemic region such as Chennai.
authors.affiliation: Department of Zoology, University of Madras, Guindy Campus, Chennai, India.
authors.forename: Veeramani
authors.initials: V
authors.lastname: Velayutham
authors.forename: Sakthivelkumar
authors.initials: S
authors.lastname: Shanmugavel
authors.forename: Arumugam
authors.initials: A
authors.lastname: Munusamy
authors.forename: Janarthanan
authors.initials: J
authors.lastname: Sundaram
mesh_terms: D000818:Animals; D001483:Base Sequence; D016000:Cluster Analysis; D005260:Female; D014644:Genetic Variation; D007194:India; D010802:Phylogeny; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique / Q000662:veterinary; D048494:Rhipicephalus / Q000235:genetics*; D017422:Sequence Analysis, DNA