title: Synthesis and analysis of linear ubiquitin chains.
abstract: Previously, polyubiquitin chains have been believed to be generated through isopeptide linkages between C-terminal of carboxyl group of ubiquitin and ε-amino group of one of the seven lysine residues in another ubiquitin. In 2006, a new type of polyubiquitin chain was identified in which the C-terminal carboxyl group of one ubiquitin is conjugated to α-amino group of the N-terminal methionine of another ubiquitin. The new type of polyubiquitin was named as the linear polyubiquitin chain. Linear polyubiquitin chains were shown to be involved in NF-κB activation. Here, we describe methods to synthesize linear polyubiquitin chains in vitro and to detect linear chains in vivo.
authors.affiliation: Cell Biology and Metabolism Group, Graduate School of Frontier Biosciences, Osaka University, Suita, Japan. kiwai@cellbio.med.osaka-u.ac.jp
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Iwai
mesh_terms: D006801:Humans; D051550:I-kappa B Kinase / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D025821:Polyubiquitin / Q000032:analysis* / Q000138:chemical synthesis* / Q000737:chemistry; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism
chemical_list: D016328:NF-kappa B; D025821:Polyubiquitin; D044767:Ubiquitin-Protein Ligases; D051550:I-kappa B Kinase