title: CMC-modified cellulose biointerface for antibody conjugation.
abstract: In this Article, we present a new strategy for preparing an antihemoglobin biointerface on cellulose. The preparation method is based on functionalization of the cellulose surface by the irreversible adsorption of CMC, followed by covalent linking of antibodies to CMC. This would provide the means for affordable and stable cellulose-based biointerfaces for immunoassays. The preparation and characterization of the biointerface were studied on Langmuir-Schaefer cellulose model surfaces in real time using the quartz crystal microbalance with dissipation and surface plasmon resonance techniques. The stable attachment of antihemoglobin to adsorbed CMC was achieved, and a linear calibration of hemoglobin was obtained. CMC modification was also observed to prevent nonspecific protein adsorption. The antihemoglobin-CMC surface regenerated well, enabling repeated immunodetection cycles of hemoglobin on the same surface.
authors.affiliation: Aalto University, School of Chemical Technology, Department of Forest Products Technology, Espoo, Finland. hannes.orelma@aalto.fi
authors.forename: Hannes
authors.initials: H
authors.lastname: Orelma
authors.forename: Tuija
authors.initials: T
authors.lastname: Teerinen
authors.forename: Leena-Sisko
authors.initials: LS
authors.lastname: Johansson
authors.forename: Susanna
authors.initials: S
authors.lastname: Holappa
authors.forename: Janne
authors.initials: J
authors.lastname: Laine
mesh_terms: D000327:Adsorption; D000906:Antibodies / Q000737:chemistry* / Q000276:immunology; D000937:Antigen-Antibody Reactions; D002482:Cellulose / Q000031:analogs & derivatives / Q000737:chemistry* / Q000276:immunology; D006454:Hemoglobins / Q000737:chemistry / Q000276:immunology; D007118:Immunoassay; D018796:Immunoconjugates / Q000737:chemistry* / Q000276:immunology; D013499:Surface Properties
chemical_list: D000906:Antibodies; D006454:Hemoglobins; D018796:Immunoconjugates; D002482:Cellulose