title: Autologous adipocyte derived stem cells favour healing in a minipig model of cutaneous radiation syndrome.
abstract: Cutaneous radiation syndrome (CRS) is the delayed consequence of localized skin exposure to high doses of ionizing radiation. Here we examined for the first time in a large animal model the therapeutic potential of autologous adipose tissue-derived stroma cells (ASCs). For experiments, Göttingen minipigs were locally gamma irradiated using a (60)Co source at the dose of 50 Gy and grafted (n = 5) or not (n = 8). ASCs were cultured in MEM-alpha with 10% fetal calf serum and basic fibroblast growth factor (2 ng.mL(-1)) and post irradiation were intradermally injected on days 25, 46, 67 and finally between days 95 and 115 (50 × 10(6) ASCs each time) into the exposed area. All controls exhibited a clinical evolution with final necrosis (day 91). In grafted pigs an ultimate wound healing was observed in four out of five grafted animals (day 130 +/- 28). Immunohistological analysis of cytokeratin expression showed a complete epidermis recovery. Grafted ASCs accumulated at the dermis/subcutis barrier in which they attracted numerous immune cells, and even an increased vasculature in one pig. Globally this study suggests that local injection of ASCs may represent a useful strategy to mitigate CRS.
authors.affiliation: Institut de Recherche Biomédicale des Armées-antenne La Tronche, La Tronche, France.
authors.forename: Fabien
authors.initials: F
authors.lastname: Forcheron
authors.forename: Diane
authors.initials: D
authors.lastname: Agay
authors.forename: Harry
authors.initials: H
authors.lastname: Scherthan
authors.forename: Diane
authors.initials: D
authors.lastname: Riccobono
authors.forename: Francis
authors.initials: F
authors.lastname: Herodin
authors.forename: Viktor
authors.initials: V
authors.lastname: Meineke
authors.forename: Michel
authors.initials: M
authors.lastname: Drouet
mesh_terms: D017667:Adipocytes / Q000166:cytology*; D000818:Animals; D005720:Gamma Rays; D011832:Radiation Injuries / Q000628:therapy*; D012867:Skin / Q000528:radiation effects*; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000166:cytology*; D013552:Swine; D013556:Swine, Miniature; D014182:Transplantation, Autologous; D016896:Treatment Outcome; D014945:Wound Healing