title: 3-O-acetyloleanolic acid induces apoptosis in human colon carcinoma HCT-116 cells.
abstract: The cytotoxic effect of 3-O-acetyloleanolic acid, an oleanolic acid derivative isolated from the seeds of Vigna sinensis K., was investigated in human colon carcinoma HCT-116 cells. 3-O-acetyloleanolic acid dose-dependently inhibited the viability of HCT-116 cells. Apoptosis was characterized by detection of cell surface annexin V and sub-G1 apoptotic cell populations. The number of immunostained cells with annexin V-FITC was increased after treatment with 3-O-acetyloleanolic acid. The sub-G1 cell population was also increased. Expression of TRAIL-mediated apoptosis signaling-related death receptor DR5 was increased in 3-O-acetyloleanolic acid-treated HCT-116 cells. Activation of caspase-8 and caspase-3, critical mediators of extrinsic apoptosis signaling, was also increased by 3-O-acetyloleanolic acid. The results indicate that 3-O-acetyloleanolic acid induces apoptosis in HCT-116 cells mediated by an extrinsic apoptosis signaling cascade via up-regulation of DR5.
authors.affiliation: Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University, Yongin, Korea.
authors.forename: Ki Hyun
authors.initials: KH
authors.lastname: Yoo
authors.forename: Jong-Hwa
authors.initials: JH
authors.lastname: Park
authors.forename: En Ji
authors.initials: EJ
authors.lastname: Cui
authors.forename: Kyung Il
authors.initials: KI
authors.lastname: Kim
authors.forename: Joo Yun
authors.initials: JY
authors.lastname: Kim
authors.forename: Jiyoung
authors.initials: J
authors.lastname: Kim
authors.forename: Seong Gil
authors.initials: SG
authors.lastname: Hong
authors.forename: Nam In
authors.initials: NI
authors.lastname: Baek
authors.forename: In Sik
authors.initials: IS
authors.lastname: Chung
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D053181:Caspase 8 / Q000378:metabolism; D007887:Fabaceae / Q000737:chemistry; D045325:HCT116 Cells; D006801:Humans; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand / Q000378:metabolism; D012639:Seeds / Q000737:chemistry; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D015854:Up-Regulation
chemical_list: D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand; D014315:Triterpenes; C052658:oleanolic acid 3-acetate; C505407:CASP3 protein, human; C505530:CASP8 protein, human; D053148:Caspase 3; D053181:Caspase 8