title: Transformation of oil palm using Agrobacterium tumefaciens.
abstract: Transgenic oil palm (Elaeis guineensis Jacq.) plantlets are regenerated after Agrobacterium tumefaciens-mediated transformation of embryogenic calli derived from young leaves of oil palm. The calli are transformed with an Agrobacterium strain, LBA4404, harboring the plasmid pUBA, which carries a selectable marker gene (bar) for resistance to the herbicide Basta and is driven by a maize ubiquitin promoter. Modifications of the transformation method, treatment of the target tissues using acetosyringone, exposure to a plasmolysis medium, and physical injury via biolistics are applied. The main reasons for such modifications are to activate the bacterial virulence system and, subsequently, to increase the transformation efficiency. Transgenic oil palm cells are selected and regenerated on a medium containing herbicide Basta. Molecular analyses revealed the presence and integration of the introduced bar gene into the genome of the transformants.
authors.affiliation: Advanced Biotechnology and Breeding Centre, MPOB, Kajang, Selangor, Malaysia.
authors.forename: Abang Masli Dayang
authors.initials: AM
authors.lastname: Izawati
authors.forename: Ghulam Kadir Ahmad
authors.initials: GK
authors.lastname: Parveez
authors.forename: Mat Yunus Abdul
authors.initials: MY
authors.lastname: Masani
mesh_terms: D000098:Acetophenones / Q000494:pharmacology; D016960:Agrobacterium tumefaciens / Q000235:genetics*; D000613:Aminobutyrates / Q000494:pharmacology*; D019470:Biolistics / Q000379:methods*; D003059:Cocos / Q000235:genetics*; D018014:Gene Transfer Techniques; D053209:Herbicide Resistance / Q000235:genetics*; D006540:Herbicides / Q000494:pharmacology; D000073878:Palm Oil; D010938:Plant Oils; D030821:Plants, Genetically Modified; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D014170:Transformation, Genetic; D025801:Ubiquitin / Q000235:genetics; D003313:Zea mays / Q000235:genetics
chemical_list: D000098:Acetophenones; D000613:Aminobutyrates; D006540:Herbicides; D010938:Plant Oils; D025801:Ubiquitin; C003121:phosphinothricin; D000073878:Palm Oil; C051667:acetosyringone