title: Comparative analysis of Campylobacter lari cytolethal distending toxin (CDT) effect on HeLa cells.
abstract: We aimed to clarify if Campylobacter lari exerts a cytolethal distending toxin (CDT) effect on HeLa cells. Campylobacter cell lysates (CCLys) from C. jejuni 81-176 and urease-positive thermophilic Campylobacter (UPTC) CF89-12 and UPTC NCTC12893 isolates were shown to exert a CDT effect on HeLa cells with morphological changes examined by Giemsa staining and microscopy. However, Campylobacter lari JCM2530(T) isolate showed no effect. In addition, Campylobacter cell culture supernatant wash gave low or absent toxic effects with both C. jejuni and C. lari organisms. When western blot analysis was carried out to clarify if there was a CDTB effect in the CCLys and soluble fractions from Campylobacter isolates, which had a CDT effect on HeLa cells or did not have any effect, anti-recombinant CjCDTB antibodies identified an immunoreactively positive signal at around approximately 25 kDa on all the C. lari isolates examined, as well as the C. jejuni 81116 strain. Thus, all the Campylobacter isolates including those without any CDT effect were shown to express CDTB at the translational level.
authors.affiliation: Laboratory of Molecular Biology Graduate School of Environmental Health Sciences, Azabu University, Fuchinobe, Chuo-ku Sagamihara, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Nakajima
authors.forename: J
authors.initials: J
authors.lastname: Hirayama
authors.forename: A
authors.initials: A
authors.lastname: Tazumi
authors.forename: K
authors.initials: K
authors.lastname: Hayashi
authors.forename: E
authors.initials: E
authors.lastname: Tasaki
authors.forename: M
authors.initials: M
authors.lastname: Asakura
authors.forename: S
authors.initials: S
authors.lastname: Yamasaki
authors.forename: J E
authors.initials: JE
authors.lastname: Moore
authors.forename: B C
authors.initials: BC
authors.lastname: Millar
authors.forename: M
authors.initials: M
authors.lastname: Matsuda
mesh_terms: D001427:Bacterial Toxins / Q000378:metabolism* / Q000633:toxicity*; D015153:Blotting, Western; D044863:Campylobacter lari / Q000472:pathogenicity*; D004847:Epithelial Cells / Q000187:drug effects*; D006367:HeLa Cells; D006801:Humans; D008853:Microscopy
chemical_list: D001427:Bacterial Toxins; C067044:cytolethal distending toxin