title: [Alteration of interleukin-17/interferon-γ double positive cells in mice with Coxsackie virus induced myocarditis].
abstract: OBJECTIVE
  To observe the alteration of interleukin-17 and interferon-γ double positive cells (IL-17(+)/IFN-γ(+) cells) in mice with coxsackie virus B3 (CVB3) induced acute viral myocarditis (VMC).
  
  
  METHODS
  VMC was induced in male Balb/c mice by peritoneal injection of CVB3. Control mice received PBS injection. At 0, 1, 2, 3, 4 and 6 weeks after injection, pathological scores were determined on hematoxylin-eosin stained heart sections and flow cytometric analysis was performed to evaluate the percent of IL-17(+)/IFN-γ(+) cells among CD4(+) T cells.
  
  
  RESULTS
  Compared to control mice, the pathological scores of VMC mice were higher on CVB3 infection week 1 (1.8 ± 0.5), peaked on week 2 (2.8 ± 0.5) and declined thereafter. However, the proportion of IL-17(+)/IFN-γ(+) cells remained steadily at a low level throughout the observation period and was similar between VMC and control mice.
  
  
  CONCLUSIONS
  Our data shows that IL-17(+)/IFN-γ(+) cells might not be involved in the inflammation process of CVB3 induced VMC mice model.
authors.affiliation: Department of Cardiology, First Affiliated Hospital of Guangxi Medical University, Cardiovascular Diseases Institute, Nanning 530021, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.forename: Wei-feng
authors.initials: WF
authors.lastname: Wu
authors.forename: Yu-luan
authors.initials: YL
authors.lastname: Yan
authors.forename: Yu
authors.initials: Y
authors.lastname: Pang
authors.forename: Qing
authors.initials: Q
authors.lastname: Kong
mesh_terms: D000818:Animals; D003384:Coxsackievirus Infections / Q000276:immunology* / Q000473:pathology; D004195:Disease Models, Animal; D004770:Enterovirus; D007371:Interferon-gamma / Q000378:metabolism*; D020381:Interleukin-17 / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009205:Myocarditis / Q000276:immunology* / Q000473:pathology / Q000821:virology; D009206:Myocardium / Q000473:pathology; D058504:Th17 Cells / Q000276:immunology* / Q000378:metabolism
chemical_list: D020381:Interleukin-17; D007371:Interferon-gamma