title: Leptospire genomic diversity revealed by microarray-based comparative genomic hybridization.
abstract: Comparative genomic hybridization was used to compare genetic diversity of five strains of Leptospira (Leptospira interrogans serovars Bratislava, Canicola, and Hebdomadis and Leptospira kirschneri serovars Cynopteri and Grippotyphosa). The array was designed based on two available sequenced Leptospira reference genomes, those of L. interrogans serovar Copenhageni and L. interrogans serovar Lai. A comparison of genetic contents showed that L. interrogans serovar Bratislava was closest to the reference genomes while L. kirschneri serovar Grippotyphosa had the least similarity to the reference genomes. Cluster analysis indicated that L. interrogans serovars Bratislava and Hebdomadis clustered together first, followed by L. interrogans serovar Canicola, before the two L. kirschneri strains. Confirmed/potential virulence factors identified in previous research were also detected in the tested strains.
authors.affiliation: Department of Biology, Howard University, Washington, DC, USA. beribo@howard.edu
authors.forename: Broderick
authors.initials: B
authors.lastname: Eribo
authors.forename: Sirima
authors.initials: S
authors.lastname: Mingmongkolchai
authors.forename: Tingfen
authors.initials: T
authors.lastname: Yan
authors.forename: Padunsri
authors.initials: P
authors.lastname: Dubbs
authors.forename: Karen E
authors.initials: KE
authors.lastname: Nelson
mesh_terms: D016000:Cluster Analysis; D055028:Comparative Genomic Hybridization; D014644:Genetic Variation; D016680:Genome, Bacterial; D007919:Leptospira / Q000235:genetics*; D046228:Microarray Analysis