title: Efficient modification of floral traits by heavy-ion beam irradiation on transgenic Torenia.
abstract: While heavy-ion beam irradiation is becoming popular technology for mutation breeding in Japan, the combination with genetic manipulation makes it more convenient to create greater variation in plant phenotypes. We have succeeded in producing over 200 varieties of transgenic torenia (Torenia fournieri Lind.) from over 2,400 regenerated plants by this procedure in only 2 years. Mutant phenotypes were observed mainly in flowers and showed wide variation in colour and shape. Higher mutation rates in the transgenics compared to those in wild type indicate the synergistic effect of genetic manipulation and heavy-ion beam irradiation, which might be advantageous to create greater variation in floral traits.
authors.affiliation: National Institute of Floricultural Science, National Agriculture and Food Research Organization, Tsukuba, Ibaraki, Japan. nohtsubo@affrc.go.jp
authors.forename: Norihiro
authors.initials: N
authors.lastname: Ohtsubo
authors.forename: Katsutomo
authors.initials: K
authors.lastname: Sasaki
authors.forename: Ryutaro
authors.initials: R
authors.lastname: Aida
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Ryuto
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ichida
authors.forename: Yoriko
authors.initials: Y
authors.lastname: Hayashi
authors.forename: Tomoko
authors.initials: T
authors.lastname: Abe
mesh_terms: D000217:Acyltransferases / Q000235:genetics; D000429:Alcohol Oxidoreductases / Q000235:genetics; D000872:Anthocyanins / Q000096:biosynthesis / Q000235:genetics; D035264:Flowers / Q000235:genetics*; D014644:Genetic Variation; D020450:Heavy Ions; D009154:Mutation; D030821:Plants, Genetically Modified / Q000502:physiology; D028083:Scrophulariaceae / Q000235:genetics* / Q000502:physiology* / Q000528:radiation effects; D046509:Tissue Culture Techniques
chemical_list: D000872:Anthocyanins; D000429:Alcohol Oxidoreductases; C056570:dihydroflavanol 4-reductase; D000217:Acyltransferases; C020141:flavanone synthetase