title: Molecular and morphological characterization of a poorly known marine ciliate, Myoschiston duplicatum precht 1935: implications for phylogenetic relationships between three morphologically similar genera -- Zoothamnium, Myoschiston, and Zoothamnopsis (Ciliophora, Peritrichia, Zoothamniidae).
abstract: We studied the morphology and molecular phylogeny of Myoschiston duplicatum, a peritrich ciliate that has been recorded as an epibiont of crustaceans, but which we also identified on marine algae from Korea. The important morphological characteristics revealed by silver staining of Myoschiston species have not been described because they are rarely collected. Using morphological methods, we redescribed the type species of the genus, Myoschiston duplicatum, and provided an improved diagnosis of Myoschiston. In addition, the coding regions for nuclear small subunit (SSU) rRNA and internal transcribed spacer 1-5.8S-internal transcribed spacer 2 sequences were sequenced. Phylogenetic analyses that included available SSU rDNA sequences of peritrichs from GenBank strongly supported a position of M. duplicatum within the family Zoothamniidae. In addition, phylogenetic analyses were performed with single datasets (ITS1-5.8S-ITS2) and combined datasets (SSU rDNA + ITS1-5.8S-ITS2) to explore further the phylogenetic relationship in the family Zoothamniidae between the three morphologically similar genera-Zoothamnium, Myoschiston, and Zoothamnopsis.
authors.affiliation: Department of Biological Science, University of Ulsan, 93 Daehak-ro, Ulsan 680-749, Korea.
authors.forename: Ping
authors.initials: P
authors.lastname: Sun
authors.forename: Dapeng
authors.initials: D
authors.lastname: Xu
authors.forename: John C
authors.initials: JC
authors.lastname: Clamp
authors.forename: Mann Kyoon
authors.initials: MK
authors.lastname: Shin
mesh_terms: D000818:Animals; D001483:Base Sequence; D003386:Brachyura / Q000469:parasitology*; D016054:DNA, Protozoan / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D008969:Molecular Sequence Data; D016802:Oligohymenophorea / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D010802:Phylogeny
chemical_list: D016054:DNA, Protozoan; D021903:DNA, Ribosomal Spacer