title: An automated, parallel processing approach to biomolecular sample preparation.
abstract: Sample preparation for DNA and RNA assays is a prime candidate for laboratory automation. A novel, parallel processing device that performs the three separate liquid-handling functions necessary for such sample preparation-dispensing, pipetting, and pressurizing-is presented. The device comprises an array of fine nozzles connected by fluidic channels to automatically and precisely distribute flow between one source and an array of points. The design principles, as well as the experimental and computational methods used to develop the device, are described. Test results, including accuracy, uniformity, volume range, and timing, are presented. The functionality of the device is demonstrated by performing a solid-phase extraction of DNA with two types of microcolumns.
authors.affiliation: Fraunhofer Center for Manufacturing Innovation, Brookline, MA 02130, USA.
authors.forename: Paul
authors.initials: P
authors.lastname: Mirsky
authors.forename: Anirban
authors.initials: A
authors.lastname: Chatterjee
authors.forename: Alexis F
authors.initials: AF
authors.lastname: Sauer-Budge
authors.forename: Andre
authors.initials: A
authors.lastname: Sharon
mesh_terms: D057205:Automation, Laboratory / Q000379:methods*; D019411:Clinical Laboratory Techniques / Q000379:methods*; D006801:Humans; D009696:Nucleic Acids / Q000302:isolation & purification*; D013048:Specimen Handling / Q000379:methods*
chemical_list: D009696:Nucleic Acids