title: Detection of exon skipping events in BRCA1 RNA using MLPA kit P002.
abstract: A rapid and easy method to screen for aberrant cDNA would be a very useful diagnostic tool in genetics since a fraction of the DNA variants found affect RNA splicing. The currently used RT-PCR methods require new primer combinations to study each variant that might affect splicing. Since MLPA is routinely used to detect large genomic deletions and successfully detected exon skipping events in Duchenne muscular dystrophy in cDNA, we performed a pilot study to evaluate its value for BRCA1 cDNA. The effect of puromycin, DNase I and two different DNA cleaning protocols were tested in the RNA analysis of lymphocyte cultures. We used two samples from unrelated families with two different BRCA1 exon deletion events, two healthy unrelated controls and six samples from hereditary breast/ovarian cancer syndrome (HBOC) patients without BRCA1/2 mutations. Using RNA treated with DNase I and cleaned in a column system from puromycin-treated fractions, we were able to identify the two BRCA1 deletions. Additional HBOC patients did not show additional splice events. However, we were not able to get reproducible results. Therefore, the cDNA-MLPA technique using kit BRCA1 P002 is in our hands currently not reliable enough for routine RNA analysis and needs further optimization.
authors.affiliation: Department of Clinical Genetics, Maastricht University Medical Centre, PO Box 616, 6200 MD Maastricht, The Netherlands. Rita.Brandao@maastrichtuniversity.nl
authors.forename: Rita D
authors.initials: RD
authors.lastname: Brandão
authors.forename: Demis
authors.initials: D
authors.lastname: Tserpelis
authors.forename: Encarna
authors.initials: E
authors.lastname: Gómez García
authors.forename: Marinus J
authors.initials: MJ
authors.lastname: Blok
mesh_terms: D019313:BRCA1 Protein / Q000235:genetics*; D001943:Breast Neoplasms / Q000175:diagnosis / Q000235:genetics; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis*; D017931:DNA Primers; D005091:Exons; D005260:Female; D019398:Genes, BRCA1; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D010051:Ovarian Neoplasms / Q000175:diagnosis / Q000235:genetics; D012313:RNA / Q000235:genetics; D015203:Reproducibility of Results; D017384:Sequence Deletion
chemical_list: D019313:BRCA1 Protein; C492913:BRCA1 protein, human; D017931:DNA Primers; D012313:RNA; D004247:DNA