title: [Construction of recombinant plasmid expressing S1 gene of new type of reovirus].
abstract: OBJECTIVE
  To construct the recombinant plasmid containing S1 gene of new type of reovirus, and to study the expression of protein sigma1 in Vero cells.
  
  
  METHODS
  The recombinant plasmid, named pC-S, was constructed by cloning S1 gene into vector pCAGGS/MCS. Then Vero cells were transfected with pC-S and collected at 24, 48, 72 h post transfection followed by SDS-PAGE and Western-Blot assay.
  
  
  RESULTS
  Results both SDS-PAGE and Western-Blot assay indicated that sigma1 protein could be expressed well and the highest expression level was 72 h post transfection.
  
  
  CONCLUSIONS
  Sigma1 protein could be expressed well in Vero cells by transfected with recombinant plasmid containing S1 gene, and could give some implications for subsequent research on virus-host interactions.
authors.affiliation: Liver Failure Therapeutic and Research Center, 302 Hospital of PLA, Beijing 100039, China.
authors.forename: Bing-Ke
authors.initials: BK
authors.lastname: Bai
authors.forename: Wei-Zhi
authors.initials: WZ
authors.lastname: Huang
authors.forename: Sheng-Dong
authors.initials: SD
authors.lastname: Luo
authors.forename: Yan
authors.initials: Y
authors.lastname: Hu
authors.forename: Rong
authors.initials: R
authors.lastname: Gao
authors.forename: Zhi-Jie
authors.initials: ZJ
authors.lastname: Wang
authors.forename: Qong
authors.initials: Q
authors.lastname: Huang
authors.forename: Hao-Dong
authors.initials: HD
authors.lastname: Liu
authors.forename: Pan-Yong
authors.initials: PY
authors.lastname: Mao
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D015870:Gene Expression; D010957:Plasmids / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012087:Reoviridae / Q000235:genetics*; D014709:Vero Cells; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D014764:Viral Proteins