title: Human amniotic membrane-derived epithelial stem cells display anticancer             activity in BALB/c female nude mice bearing disseminated breast cancer xenografts.
abstract: Breast cancer is one of the most common malignant tumors and the leading             cause of mortality among women. In this study, we propose a human stem cell transplantation             strategy, an important method for treating various cancers, as a potential breast             cancer therapy. To this end, we used human amniotic membrane-derived epithelial             stem cells (hAECs) as a cell source for performing human stem cell transplantation.             hAECs have multipotent differentiation abilities and possess high proliferative             potential. We transplanted hAECs into female BALB/c nude mice bearing tumors originating             from MDA-MB-231 breast cancer cells. Co-culturred hAECs and MDA-MB-231 cells at             a ratio of 1:4 or 1:8 (tumor cells to stem cells) inhibited breast cancer cell             growth by 67.29 and 67.33%, respectively. In the xenograft mouse model, tumor             volumes were significantly decreased by 5-flurouracil (5-FU) treatment and two             different ratios of hAECs (1:4 and 1:8) by 84.33, 73.88 and 56.89%, respectively.             Treatment of nude mice with hAECs (1:4) produced remarkable antitumor effects             without any side-effects (e.g., weight loss, death and bruising) compared to the             mice that received only 5-FU treatment. Tumor progression was significantly reduced             by hAEC treatment compared to the xenograft model. On the other hand, breast tissues             (e.g., the epidermis, dermis and reticular layer) appeared to be well-maintained             following treatment with hAECs. Taken together, these results provide strong evidence             that hAECs can be used as a safe and effective cancer-targeting cytotherapy for             treating breast cancer.
authors.affiliation: Laboratory of Veterinary Biochemistry and Immunology, College                     of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk,                     Republic of Korea.
authors.forename: Nam-Hee
authors.initials: NH
authors.lastname: Kang
authors.forename: Bo-Rim
authors.initials: BR
authors.lastname: Yi
authors.forename: So Yoon
authors.initials: SY
authors.lastname: Lim
authors.forename: Kyung-A
authors.initials: KA
authors.lastname: Hwang
authors.forename: Young Seok
authors.initials: YS
authors.lastname: Baek
authors.forename: Kyung-Sun
authors.initials: KS
authors.lastname: Kang
authors.forename: Kyung-Chul
authors.initials: KC
authors.lastname: Choi
mesh_terms: D000650:Amnion / Q000166:cytology*; D000818:Animals; D001943:Breast Neoplasms / Q000473:pathology / Q000628:therapy*; D045744:Cell Line, Tumor; D018920:Coculture Techniques; D016207:Cytokines / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism / Q000637:transplantation*; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D047368:Tumor Burden
chemical_list: D016207:Cytokines