title: [Expression and procoagulant activity of phosphatidylserine on the normal blood cells].
abstract: OBJECTIVE
  To investigate the expression and procoagulant activity of phosphatidylserine (PS) on the normal peripheral blood cells of adults.
  
  
  METHODS
  Normal peripheral blood samples were collected from 10 healthy volunteers (5 ml from each volunteer), platelets, neutrophils, lymphocytes and erythrocytes were isolated. The expression and procoagulant activity of PS on normal blood cells were identified by flow cytometry, inhibition test with lactadherin as PS probe and coagulation anticoagulant, respectively.
  
  
  RESULTS
  There was PS expression on a few normal blood cells (9.1%, 5.4%, 3.9% and 3.2% in platelets, neutrophils, lymphocytes and erythrocytes, respectively). The PS on these normal blood cells in vitro showed significant procoagulant activity. The plasma recalcification time was shortened by 47%, 36.5%, 25% and 12.5% by platelets, neutrophils, lymphocytes and erythrocytes, respectively; the formation of factor Xa (through both intrinsic and extrinsic pathways) and thrombin was also increased by 13% - 26% by platelets, neutrophils, lymphocytes and erythrocytes, respectively.
  
  
  CONCLUSIONS
  The PS on normal blood cells in vivo may play a crucial role in the coagulation cascade.
authors.affiliation: Department of Hematology, the First Affiliated Hospital of Harbin Medical University, China.
authors.forename: Shu-chuan
authors.initials: SC
authors.lastname: Liu
authors.forename: Jin
authors.initials: J
authors.lastname: Zhou
authors.forename: Jia-lan
authors.initials: JL
authors.lastname: Shi
authors.forename: Yan-hua
authors.initials: YH
authors.lastname: Su
mesh_terms: D000328:Adult; D001773:Blood Cells / Q000378:metabolism* / Q000502:physiology*; D001780:Blood Coagulation Tests; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D010718:Phosphatidylserines / Q000378:metabolism*
chemical_list: D010718:Phosphatidylserines