title: Worm-like Ising model for protein mechanical unfolding under the effect of osmolytes.
abstract: We show via single-molecule mechanical unfolding experiments that the osmolyte glycerol stabilizes the native state of the human cardiac I27 titin module against unfolding without shifting its unfolding transition state on the mechanical reaction coordinate. Taken together with similar findings on the immunoglobulin-binding domain of streptococcal protein G (GB1), these experimental results suggest that osmolytes act on proteins through a common mechanism that does not entail a shift of their unfolding transition state. We investigate the above common mechanism via an Ising-like model for protein mechanical unfolding that adds worm-like-chain behavior to a recent generalization of the Wako-Saitô-Muñoz-Eaton model with support for group-transfer free energies. The thermodynamics of the model are exactly solvable, while protein kinetics under mechanical tension can be simulated via Monte Carlo algorithms. Notably, our force-clamp and velocity-clamp simulations exhibit no shift in the position of the unfolding transition state of GB1 and I27 under the effect of various osmolytes. The excellent agreement between experiment and simulation strongly suggests that osmolytes do not assume a structural role at the mechanical unfolding transition state of proteins, acting instead by adjusting the solvent quality for the protein chain analyte.
authors.affiliation: Department of Biochemistry G. Moruzzi, University of Bologna, Bologna, Italy. aioaneid@gmail.com
authors.forename: Daniel
authors.initials: D
authors.lastname: Aioanei
authors.forename: Marco
authors.initials: M
authors.lastname: Brucale
authors.forename: Isabella
authors.initials: I
authors.lastname: Tessari
authors.forename: Luigi
authors.initials: L
authors.lastname: Bubacco
authors.forename: Bruno
authors.initials: B
authors.lastname: Samorì
mesh_terms: D001696:Biomechanical Phenomena; D005990:Glycerol / Q000737:chemistry; D007700:Kinetics; D055595:Mechanical Phenomena; D008958:Models, Molecular; D009995:Osmosis; D011487:Protein Conformation; D058767:Protein Unfolding; D013816:Thermodynamics
chemical_list: D005990:Glycerol