title: Clinical implications of AEG-1 in liver metastasis of colorectal cancer.
abstract: The aim of the study was to investigate the role of AEG-1 in colorectal cancer and the relationship between AEG-1 protein expression and pathological characteristics and prognosis in colorectal cancer. The AEG-1 mRNA and protein expression level between the tumor cells from the cases with liver metastasis and those from the ones without live metastasis were detected by RT-PCR and immunohistochemistry staining. The relationship between AEG-1 and clinicopathological parameters of colorectal cancer was determined. AEG-1 expressed positively in 218 (41.92%) of the 520 cases examined. It showed that both mRNA and protein level AEG-1 were over-expressed in the cases with liver metastasis compared with those without liver metastasis (P=0.01). After immunohistochemistry analysis, the expression of AEG-1 protein was related to age, Duke's stage and distant metastasis (P=0.001, 0.001 and 0.016, respectively). After survival analysis, the cases with highly expressed AEG-1 protein attained a significantly poorer postoperative disease-specific survival than those with none/low-expressed AEG-1 protein (P=0.001). In the Cox regression test, histological grade, Duke's stage and AEG-1 were detected as the independent prognostic factors (P=0.035, 0.001 and 0.010, respectively). AEG-1 protein may be a potential new early liver metastasis biomarker of colorectal cancer.
authors.affiliation: Department of Colorectal Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, 150001, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
authors.forename: Anlong
authors.initials: A
authors.lastname: Zhu
authors.forename: Yuekun
authors.initials: Y
authors.lastname: Zhu
authors.forename: Daxun
authors.initials: D
authors.lastname: Piao
mesh_terms: D000328:Adult; D000368:Aged; D015815:Cell Adhesion Molecules / Q000032:analysis / Q000502:physiology*; D015179:Colorectal Neoplasms / Q000401:mortality / Q000473:pathology*; D005260:Female; D006801:Humans; D008113:Liver Neoplasms / Q000556:secondary*; D008297:Male; D008565:Membrane Proteins; D008875:Middle Aged; D011379:Prognosis; D016601:RNA-Binding Proteins
chemical_list: D015815:Cell Adhesion Molecules; C483039:MTDH protein, human; D008565:Membrane Proteins; D016601:RNA-Binding Proteins