title: Two-in-one: combined targeted chemo and gene therapy for tumor suppression and prevention of metastases.
abstract: OBJECTIVE
  To develop an approach for the effective treatment of ovarian tumor, prevention of metastases and limitation of side effects.
  
  
  METHODS
  In order to combine chemotherapy with genotherapy, we constructed a nanoscale-based tumor-targeted system containing two anticancer drugs, two antisense oligonucleotides (suppressors of cellular resistance) and a ligand specific to receptors overexpressed in cancer cells. The system was tested in a mouse metastatic xenograft model using tumor isolates from patients with ovarian carcinoma.
  
  
  RESULTS
  Treatment with the proposed nanosystem decreased primary tumor, prevented intraperitoneal metastases and limited adverse side effects.
  
  
  CONCLUSIONS
  The advantages of the proposed targeted complex treatment of primary aggressive ovarian tumor and prevention of metastases make the proposed approach promising for clinical applications.
authors.affiliation: Department of Pharmaceutics, Rutgers, The State University of New Jersey, 160 Frelinghuysen Road, Piscataway, NJ 08854, USA.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Olga B
authors.initials: OB
authors.lastname: Garbuzenko
authors.forename: Kenneth R
authors.initials: KR
authors.lastname: Reuhl
authors.forename: Lorna
authors.initials: L
authors.lastname: Rodriguez-Rodriguez
authors.forename: Tamara
authors.initials: T
authors.lastname: Minko
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage*; D002277:Carcinoma / Q000473:pathology / Q000556:secondary* / Q000628:therapy*; D003131:Combined Modality Therapy; D005260:Female; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D058990:Molecular Targeted Therapy / Q000379:methods*; D053769:Nanocapsules / Q000737:chemistry / Q000627:therapeutic use*; D010051:Ovarian Neoplasms / Q000473:pathology / Q000628:therapy*; D016896:Treatment Outcome; D014407:Tumor Cells, Cultured
chemical_list: D000970:Antineoplastic Agents; D053769:Nanocapsules