title: A multifunctional teal-fluorescent Rosa26 reporter mouse line for Cre- and Flp-mediated recombination.
abstract: Reporters of Cre and/or Flp activity are important for defining the spatial and temporal extent of Cre/Flp-mediated recombination. Here, we describe R26-CAG-LF-mTFP1, a multifunctional fluorescent reporter mouse that strongly expresses mTFP1 (bright teal fluorescent protein) after Cre- and Flp-mediated recombination. To meet the need for single recombinase-mediated reporter expression, we generated derivatives of R26-CAG-LF-mTFP1. The germline excision of the Frt-flanked stop cassette in R26-CAG-LF-mTFP1 generated a Cre-dependent reporter (R26-CAG-LoxP-mTFP1). Similarly, R26-CAG-FRT-mTFP1, in which the loxP-flanked stop cassette was excised in the germline, requires only Flp to activate mTFP1 expression.
authors.affiliation: Institute for Virus Research, Kyoto University, Kyoto, Japan. iimayosh@virus.kyoto-u.ac.jp
authors.forename: Itaru
authors.initials: I
authors.lastname: Imayoshi
authors.forename: Kyoko
authors.initials: K
authors.lastname: Hirano
authors.forename: Masayuki
authors.initials: M
authors.lastname: Sakamoto
authors.forename: Goichi
authors.initials: G
authors.lastname: Miyoshi
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Imura
authors.forename: Satsuki
authors.initials: S
authors.lastname: Kitano
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Miyachi
authors.forename: Ryoichiro
authors.initials: R
authors.lastname: Kageyama
mesh_terms: D000818:Animals; D004254:DNA Nucleotidyltransferases / Q000502:physiology*; D055879:Gene Knock-In Techniques; D017930:Genes, Reporter / Q000502:physiology*; D049452:Green Fluorescent Proteins / Q000502:physiology*; D019426:Integrases / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D011506:Proteins / Q000502:physiology*; D022661:RNA, Untranslated; D011995:Recombination, Genetic / Q000502:physiology*
chemical_list: C110853:Gt(ROSA)26Sor non-coding RNA, mouse; D011506:Proteins; D022661:RNA, Untranslated; D049452:Green Fluorescent Proteins; C045073:Cre recombinase; D004254:DNA Nucleotidyltransferases; C045119:FLP recombinase; D019426:Integrases