title: Identification of proteins that interact with murine cytomegalovirus early protein M112-113 in brain.
abstract: BACKGROUND
  Murine cytomegalovirus (MCMV) early protein M112-113 is involved in viral DNA replication and believed to play a crucial role in the viral pathogenesis. To investigate the biological function of M112-113 protein in the pathogenesis of the brain disorders caused by cytomegalovirus (CMV), a screening for proteins interacting with M112-113 was performed by a yeast two-hybrid system.
  
  
  METHODS
  Bait plasmid pGBKT7-M112-113 was constructed and transformed into AH109 yeast. After confirmation of the expression of MCMV M112-113 in yeast, the bait yeast was mated with a prey yeast containing mouse brain cDNA library plasmid to screen the proteins interacting with M112-113. Interactions between M112-113 and the obtained proteins were verified by yeast two-hybrid assay and chemiluminescent co-immunoprecipitaion.
  
  
  RESULTS
  Two proteins interacting with M112-113 were identified, including metastasis-associated 1 (MTA1) and zinc finger, CCHC domain containing 18 (ZCCHC18). M112-113 protein could interact with MTA1 or ZCCHC18 in yeast and mammalian cells.
  
  
  CONCLUSIONS
  The interactions of M112-113 with MTA1 or ZCCHC18 may be related to the pathogenesis of MCMV-associated disease in central nervous system.
authors.affiliation: Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Xing-Lou
authors.initials: XL
authors.lastname: Liu
authors.forename: Sai-Nan
authors.initials: SN
authors.lastname: Shu
authors.forename: Yong-Jian
authors.initials: YJ
authors.lastname: Huang
authors.forename: Feng
authors.initials: F
authors.lastname: Fang
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002460:Cell Line; D006801:Humans; D047468:Immunoprecipitation; D051379:Mice; D018146:Muromegalovirus / Q000378:metabolism*; D010957:Plasmids; D011485:Protein Binding; D020798:Two-Hybrid System Techniques; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D014764:Viral Proteins