title: MicroRNA-351 regulates TMEM 59 (DCF1) expression and mediates neural stem cell morphogenesis.
abstract: MicroRNAs (miRNA) are small noncoding RNAs that play important roles in cell development, differentiation and apoptosis. Recent studies showed that transmembrane protein 59 (TMEM59) affects neural stem cell (NSC) differentiation and β-amyloid precursor protein (APP) glycosylation. In this study, we predicted that microRNA-351 (miR-351) could target TMEM59 and demonstrated that miR-351 negatively regulates TMEM59 expression in different cell types. Moreover, we found that miR-351 overexpression could lead to morphological change in the mouse NSC cell line C17.2, indicating an important role of miR-351 in the regulation of differentiation. Our investigations focused on the functions of miR-351 in the nervous system and the posttranscriptional regulation of TMEM59 by microRNA. These original findings provide promising grounds for future in-depth research into the functions of miR-351 and TMEM59 in nervous system development.
authors.affiliation: Laboratory of Molecular Neural Biology, School of Life Sciences and Institute of Systems Biology, Shanghai University, Shanghai, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Li
authors.forename: Ruili
authors.initials: R
authors.lastname: Feng
authors.forename: Chen
authors.initials: C
authors.lastname: Huang
authors.forename: Haifeng
authors.initials: H
authors.lastname: Wang
authors.forename: Jiao
authors.initials: J
authors.lastname: Wang
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Zhang
authors.forename: Huang
authors.initials: H
authors.lastname: Yan
authors.forename: Tieqiao
authors.initials: T
authors.lastname: Wen
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D015870:Gene Expression; D057809:HEK293 Cells; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D008954:Models, Biological; D009024:Morphogenesis / Q000235:genetics*; D009419:Nerve Tissue Proteins / Q000235:genetics*; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D014162:Transfection
chemical_list: D008565:Membrane Proteins; D035683:MicroRNAs; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C580764:MIRN351 microRNA, 351; C000626540:Tmem59 protein, mouse