title: Study of clinical, biochemical and immunological factors determining stability of disease in patients with generalized vitiligo undergoing melanocyte transplantation.
abstract: BACKGROUND
  Stability is considered the most important parameter before performing any melanocyte transplantation procedure in vitiligo; however, current criteria rely on the history given by the patients.
  
  
  OBJECTIVE
  This study was undertaken to determine the clinical, biochemical and immunological factors determining stability of disease in patients with generalized vitiligo to facilitate better patient selection for melanocyte transplantation and to understand immunological mechanisms for disease activity.
  
  
  METHODS
  Thirty-three patients with generalized vitiligo with < 10% body surface area involved were allocated to three clinical stability groups: Group 1 (stability > 3 months but < 1 year), Group 2 (≥ 1 year but < 2 years) and Group 3 (≥ 2 years). Melanocyte transplantation was done using suction blister epidermal grafting (SBEG) on a single patch. Blood was drawn for catalase estimation from all patients and from 10 healthy control subjects. A 3-mm punch biopsy was taken on the day of transplantation from the margin of the macule in the first five patients in each group for the immunohistochemistry of CD4, CD8, CD45RO, CD45RA and FoxP3. Those with ≥ 75% repigmentation at 6 months were labelled as responders.
  
  
  RESULTS
  The success rate was 0% in Group 1, 37·5% in Group 2 and 77·8% in Group 3. The difference in the success rate between the groups was statistically significant (P = 0·005). The median period of stability was significantly higher in the responders compared with that in the nonresponders (P = 0·001). Catalase levels were not significantly different between patients in the three groups of cases and in controls, or between responders and nonresponders. Lesional CD8 cells were significantly higher in Group 1 compared with Group 3. The percentages of CD8 and CD45RO cells were significantly higher in the nonresponders compared with the responders.
  
  
  CONCLUSIONS
  Along with clinical stability, the proportion of CD8 and CD45RO cells in skin biopsies might help to determine the stability of the disease and thereby predict the success of transplantation.
authors.affiliation: Department of Dermatology and Venereology, All India Institute of Medical Sciences, New Delhi-110029, India.
authors.forename: A
authors.initials: A
authors.lastname: Rao
authors.forename: S
authors.initials: S
authors.lastname: Gupta
authors.forename: A K
authors.initials: AK
authors.lastname: Dinda
authors.forename: A
authors.initials: A
authors.lastname: Sharma
authors.forename: V K
authors.initials: VK
authors.lastname: Sharma
authors.forename: G
authors.initials: G
authors.lastname: Kumar
authors.forename: D K
authors.initials: DK
authors.lastname: Mitra
authors.forename: C K
authors.initials: CK
authors.lastname: Prashant
authors.forename: G
authors.initials: G
authors.lastname: Singh
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002374:Catalase / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017493:Leukocyte Common Antigens / Q000276:immunology; D008297:Male; D008544:Melanocytes / Q000637:transplantation*; D008875:Middle Aged; D013601:T-Lymphocytes / Q000276:immunology; D016896:Treatment Outcome; D014820:Vitiligo / Q000201:enzymology / Q000276:immunology / Q000628:therapy*; D055815:Young Adult
chemical_list: D002374:Catalase; D017493:Leukocyte Common Antigens; C516769:PTPRC protein, human