title: In vitro and in vivo characteristics of gentamicin-supplemented polyvinylidenfluoride mesh materials.
abstract: To reduce infection rates after mesh implantation antibiotic-coated meshes were designed. The aim of the study was to analyze biocompatibility and in vitro efficiency of a modified gentamicin-supplemented polyvinylidenfluoride mesh. Twenty rats were randomized to two groups (PVDF group and Genta group). Mesh material was implanted subcutaneously. Blood samples were taken to determine the gentamicin serum concentration. Seven and 90 days after mesh implantation, animals were euthanized. The inflammatory tissue response was characterized by analyzing the foreign body granuloma. Cellular immune response was analyzed by immunohistochemical investigations. The collagen type I/III ratio was estimated by crosspolarization microscopy. In vitro agar diffusion test, suspension test, and gentamicin release were characterized. Agar diffusion and suspension test showed efficient antibiotic effects of the mesh in vitro. Serum concentrations of gentamicin showed a peak value 1 h postoperatively with a decline within the next day. The total size of the granuloma was significantly smaller in the Genta group compared to the PVDF group at both points of time. Except of a short period of increased expression of CD68 in the Genta group after 7 days, no further difference was found analyzing cellular immune response. The collagen type I/III ratio was widely constant analyzing the two mesh types without significant differences comparing both mesh materials. A significantly decreased foreign body granuloma formation compared to the pure PVDF mesh group was found. In vitro analysis showed efficient antibiotic effects of the Gentamicin supplementation compared to the pure PVDF mesh.
authors.affiliation: Department of Surgery, RWTH Aachen, Germany. cklink@ukaachen.de
authors.forename: C D
authors.initials: CD
authors.lastname: Klink
authors.forename: M
authors.initials: M
authors.lastname: Binnebösel
authors.forename: A
authors.initials: A
authors.lastname: Lambertz
authors.forename: H P
authors.initials: HP
authors.lastname: Alizai
authors.forename: A
authors.initials: A
authors.lastname: Roeth
authors.forename: J
authors.initials: J
authors.lastname: Otto
authors.forename: U
authors.initials: U
authors.lastname: Klinge
authors.forename: U P
authors.initials: UP
authors.lastname: Neumann
authors.forename: K
authors.initials: K
authors.lastname: Junge
mesh_terms: D000362:Agar; D000818:Animals; D024042:Collagen Type I / Q000378:metabolism; D024061:Collagen Type III / Q000378:metabolism; D004058:Diffusion; D005839:Gentamicins / Q000097:blood / Q000494:pharmacology*; D015745:Granuloma, Foreign-Body / Q000473:pathology; D007150:Immunohistochemistry; D008297:Male; D008422:Materials Testing; D008826:Microbial Sensitivity Tests; D011145:Polyvinyls / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D013211:Staphylococcus aureus / Q000187:drug effects; D013526:Surgical Mesh; D013997:Time Factors; D005465:Fluorocarbon Polymers
chemical_list: D000362:Agar; D024042:Collagen Type I; D024061:Collagen Type III; D005839:Gentamicins; D011145:Polyvinyls; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers