title: Dynamic oxidative stress and DNA damage induced by oestrogen deficiency and protective effects of puerarin and 17β-oestradiol in ovariectomized rats.
abstract: Oxidative stress plays an essential role in the pathogenesis of cardiovascular diseases and osteoporosis resulting from oestrogen deficiency in the postmenopausal period. In this report, we observed a dynamic change of oxidative stress and DNA damage after ovariectomy in female rats. We then compared phytoestrogen puerarin and 17β-oestradiol (E₂) in their effects on oestrogen deficiency-induced oxidative stress and DNA damage. Serum total antioxidant capacity (TAC), malondialdehyde (MDA) and lymphocytes DNA damage (comet%) were measured. There was a gradual increase in oxidative stress in the ovariectomized (OVX) rats over time after ovariectomy, as compared to rats receiving sham operation. OVX rats that were on puerarin and E₂ showed increased TAC and decreased MDA in the serum, as well as decreased lymphocytes comet%. Puerarin appeared to have a more powerful protective effect on DNA oxidative damage than E₂. The study indicates that postmenopausal women may benefit from phytoestrogen puerarin.
authors.affiliation: Department of Endocrinology, The First Hospital of Lanzhou University, China. xulei_tang@126.com
authors.forename: Xu-Lei
authors.initials: XL
authors.lastname: Tang
authors.forename: Xiao-Ju
authors.initials: XJ
authors.lastname: Liu
authors.forename: Quan
authors.initials: Q
authors.lastname: Tian
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D004958:Estradiol / Q000494:pharmacology*; D004967:Estrogens / Q000172:deficiency*; D005260:Female; D007529:Isoflavones / Q000494:pharmacology*; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects; D008315:Malondialdehyde / Q000097:blood; D010052:Ovariectomy / Q000379:methods; D018384:Oxidative Stress / Q000187:drug effects*; D048789:Phytoestrogens / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000975:Antioxidants; D004967:Estrogens; D007529:Isoflavones; D048789:Phytoestrogens; D004958:Estradiol; D008315:Malondialdehyde; C033607:puerarin