title: Engineering of silicon surfaces at the micro- and nanoscales for cell adhesion and migration control.
abstract: The engineering of surface patterns is a powerful tool for analyzing cellular communication factors involved in the processes of adhesion, migration, and expansion, which can have a notable impact on therapeutic applications including tissue engineering. In this regard, the main objective of this research was to fabricate patterned and textured surfaces at micron- and nanoscale levels, respectively, with very different chemical and topographic characteristics to control cell-substrate interactions. For this task, one-dimensional (1-D) and two-dimensional (2-D) patterns combining silicon and nanostructured porous silicon were engineered by ion beam irradiation and subsequent electrochemical etch. The experimental results show that under the influence of chemical and morphological stimuli, human mesenchymal stem cells polarize and move directionally toward or away from the particular stimulus. Furthermore, a computational model was developed aiming at understanding cell behavior by reproducing the surface distribution and migration of human mesenchymal stem cells observed experimentally.
authors.affiliation: Department of Applied Physics, Universidad Autónoma de Madrid, Madrid, Spain.
authors.forename: Vicente
authors.initials: V
authors.lastname: Torres-Costa
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Martínez-Muñoz
authors.forename: Vanessa
authors.initials: V
authors.lastname: Sánchez-Vaquero
authors.forename: Álvaro
authors.initials: Á
authors.lastname: Muñoz-Noval
authors.forename: Laura
authors.initials: L
authors.lastname: González-Méndez
authors.forename: Esther
authors.initials: E
authors.lastname: Punzón-Quijorna
authors.forename: Darío
authors.initials: D
authors.lastname: Gallach-Pérez
authors.forename: Miguel
authors.initials: M
authors.lastname: Manso-Silván
authors.forename: Aurelio
authors.initials: A
authors.lastname: Climent-Font
authors.forename: Josefa P
authors.initials: JP
authors.lastname: García-Ruiz
authors.forename: Raúl J
authors.initials: RJ
authors.lastname: Martín-Palma
mesh_terms: D002448:Cell Adhesion / Q000502:physiology*; D018929:Cell Culture Techniques / Q000295:instrumentation*; D002465:Cell Movement / Q000502:physiology*; D003198:Computer Simulation; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology; D008856:Microscopy, Fluorescence; D049329:Nanostructures / Q000648:ultrastructure*; D036103:Nanotechnology; D016062:Porosity; D012825:Silicon / Q000737:chemistry*; D013499:Surface Properties; D023822:Tissue Engineering / Q000295:instrumentation
chemical_list: D012825:Silicon