title: Characterization of influenza A virus with nine segments: effect gene segment on virus property.
abstract: The influenza A virus genome consists of eight segments of negative-strand RNA. In previous study, we generated a recombinant influenza virus with nine segments by reverse genetics. In present study, we evaluated characteristics of the recombinant influenza virus. The recombinant virus exhibited similar property to wild-type virus on virion morphology, virion composition, plaque phenotype and other aspects. Whereas, the recombinant virus propagated to lower titers than did wild-type virus in cells and mice, and there was decreased protein level and vRNA incorporation in the recombinant virions compared to wild-type H9N2 virions. Our results indicated that influenza A virus with eight segments exhibits more advantages than the virus with nine segments.
authors.affiliation: Institute of Animal Husbandry and Veterinary Science, Hubei Academy of Agricultural Sciences, Wuhan 430064, Hubei, China. xudiping2007@126.com
authors.forename: Rui
authors.initials: R
authors.lastname: Wu
authors.forename: Xuke
authors.initials: X
authors.lastname: Zhang
authors.forename: Huabin
authors.initials: H
authors.lastname: Shao
authors.forename: Qingping
authors.initials: Q
authors.lastname: Luo
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Diping
authors.initials: D
authors.lastname: Xu
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D017353:Gene Deletion; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D053127:Influenza A Virus, H9N2 Subtype / Q000235:genetics*; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D016254:Mutagenesis, Insertional; D009976:Orthomyxoviridae Infections / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics; D014771:Virion
chemical_list: D012367:RNA, Viral