title: Transauricular embolization of the rabbit coronary artery for experimental myocardial infarction: comparison of a minimally invasive closed-chest model with open-chest surgery.
abstract: BACKGROUND
  To date, most animal studies of myocardial ischemia have used open-chest models with direct surgical coronary artery ligation. We aimed to develop a novel, percutaneous, minimally-invasive, closed-chest model of experimental myocardial infarction (EMI) in the New Zealand White rabbit and compare it with the standard open-chest surgical model in order to minimize local and systemic side-effects of major surgery.
  
  
  METHODS
  New Zealand White rabbits were handled in conformity with the "Guide for the Care and Use of Laboratory Animals" and underwent EMI under intravenous anesthesia. Group A underwent EMI with an open-chest method involving surgical tracheostomy, a mini median sternotomy incision and left anterior descending (LAD) coronary artery ligation with a plain suture, whereas Group B underwent EMI with a closed-chest method involving fluoroscopy-guided percutaneous transauricular intra-arterial access, superselective LAD catheterization and distal coronary embolization with a micro-coil. Electrocardiography (ECG), cardiac enzymes and transcatheter left ventricular end-diastolic pressure (LVEDP) measurements were recorded. Surviving animals were euthanized after 4 weeks and the hearts were harvested for Hematoxylin-eosin and Masson-trichrome staining.
  
  
  RESULTS
  In total, 38 subjects underwent EMI with a surgical (n = 17) or endovascular (n = 21) approach. ST-segment elevation (1.90 ± 0.71 mm) occurred sharply after surgical LAD ligation compared to progressive ST elevation (2.01 ± 0.84 mm;p = 0.68) within 15-20 min after LAD micro-coil embolization. Increase of troponin and other cardiac enzymes, abnormal ischemic Q waves and LVEDP changes were recorded in both groups without any significant differences (p > 0.05). Infarct area was similar in both models (0.86 ± 0.35 cm in the surgical group vs. 0.92 ± 0.54 cm in the percutaneous group;p = 0.68).
  
  
  CONCLUSIONS
  The proposed model of transauricular coronary coil embolization avoids thoracotomy and major surgery and may be an equally reliable and reproducible platform for the experimental study of myocardial ischemia.
authors.affiliation: Department of Interventional Radiology, Patras University Hospital, School of Medicine, 26504, Rion, Greece. katsanos@med.upatras.gr
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Katsanos
authors.forename: Sofoklis
authors.initials: S
authors.lastname: Mitsos
authors.forename: Efstratios
authors.initials: E
authors.lastname: Koletsis
authors.forename: Vassiliki
authors.initials: V
authors.lastname: Bravou
authors.forename: Dimitris
authors.initials: D
authors.lastname: Karnabatidis
authors.forename: Fevronia
authors.initials: F
authors.lastname: Kolonitsiou
authors.forename: Athanassios
authors.initials: A
authors.lastname: Diamantopoulos
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Dougenis
authors.forename: Dimitris
authors.initials: D
authors.lastname: Siablis
mesh_terms: D000818:Animals; D006348:Cardiac Surgical Procedures / Q000379:methods; D004195:Disease Models, Animal; D004617:Embolism; D019060:Minimally Invasive Surgical Procedures; D009203:Myocardial Infarction; D011817:Rabbits