title: Endometrial glandular and stromal breakdown, Part 4: Cytomorphology of "condensed cluster of stromal cells including a light green body".
abstract: This study was undertaken to clarify the origin of the chromophilic substance that stained in light green [light green body (LGB)] was observed in the condensed cluster of stromal cells and in the background of endometrial glandular and stromal breakdown cases. The material consists of cytologic smears of endometrial glandular and stromal breakdown (EGBD) from 58 samples in which a histopathological diagnosis was subsequently obtained by endometrial curettage. The following parameters were examined. (1) The occurrence and number of a LGB. As for the "condensed cluster of stromal cells," "condensed cluster of stromal cells including LGB" and "LBG in background," it was observed with all fields on one slide per case. When it was observed more than one in a preparation, the occurrence was determined and the number was also calculated. (2) Immunostaining of paraffin-embedded tissue sections and cytologic preparations. 1 The occurrence of "condensed cluster of stromal cells," "condensed cluster of stromal cells including LGB" and "LGB in background" was 100%, 44.8% and 91.4%, respectively. The number of "condensed cluster of stromal cells," "condensed cluster of stromal cells including LGB" and "LGB in background" was 21.2, 2.0 and 4.0, respectively. 2 When histological sections from paraffin-embedded tissue were tested by immunocytochemistry, "LGB" stained weakly or negative for fibrinogen, whereas it stained positively for CD31 and factor VIII. In addition, it stained positively for CD42b. 3 When cytologic preparation was tested by immunocytochemistry, "LGB" stained positively for CD31, factor VIII and CD42b. However, as for the fibrinogen, quantitative and qualitative evaluation of the immunocytochemical stain was not feasible due to extensive nonspecific reaction in the whole preparation. The "LGB" in EGBD cases proved to be a thrombus mostly made up by platelets, and it seemed that the recognition of the LGB showed to be a useful cytomorphological criterion for an always more accurate diagnosis of EGBD cases.
authors.affiliation: Department of Medical Technology, Ehime Prefectural University of Health Sciences, Ehime, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Norimatsu
authors.forename: Miho
authors.initials: M
authors.lastname: Kawai
authors.forename: Akira
authors.initials: A
authors.lastname: Kamimori
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Yuminamochi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ohsaki
authors.forename: Kenji
authors.initials: K
authors.lastname: Yanoh
authors.forename: Namiki
authors.initials: N
authors.lastname: Kawanishi
authors.forename: Tadao K
authors.initials: TK
authors.lastname: Kobayashi
mesh_terms: D000328:Adult; D003937:Diagnosis, Differential; D016889:Endometrial Neoplasms / Q000473:pathology*; D004717:Endometrium / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D017154:Stromal Cells / Q000473:pathology*; D014591:Uterine Diseases / Q000473:pathology*; D014626:Vaginal Smears