title: Europium-labeled activity-based probe through click chemistry: absolute serine protease quantification using (153)Eu isotope dilution ICP/MS.
abstract: Click and analyze: the titled probe was synthesized by conjugating a sulfonyl fluoride and azido unit using click chemistry to give SF-Eu, which can react specifically with serine (Ser) in the active site of serine protease (SP). Combination of the method with (153)Eu-isotope dilution ICP/MS enables absolute protein quantification of active SPs in biological samples using only one (153)Eu(NO(3))(3) isotopic standard.
authors.affiliation: Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, China.
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Yan
authors.forename: Yacui
authors.initials: Y
authors.lastname: Luo
authors.forename: Zhubao
authors.initials: Z
authors.lastname: Zhang
authors.forename: Zhaoxin
authors.initials: Z
authors.lastname: Li
authors.forename: Qiang
authors.initials: Q
authors.lastname: Luo
authors.forename: Limin
authors.initials: L
authors.lastname: Yang
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.forename: Haifeng
authors.initials: H
authors.lastname: Chen
authors.forename: Peiming
authors.initials: P
authors.lastname: Bai
authors.forename: Qiuquan
authors.initials: Q
authors.lastname: Wang
mesh_terms: D020134:Catalytic Domain; D002851:Chromatography, High Pressure Liquid; D057930:Click Chemistry; D005063:Europium / Q000737:chemistry*; D006573:Heterocyclic Compounds, 1-Ring / Q000737:chemistry; D007553:Isotope Labeling; D013058:Mass Spectrometry; D057057:Serine Proteases / Q000032:analysis*; D013441:Sulfinic Acids / Q000737:chemistry
chemical_list: D006573:Heterocyclic Compounds, 1-Ring; D013441:Sulfinic Acids; C071349:1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid; D005063:Europium; C048899:sulfuryl fluoride; D057057:Serine Proteases