title: Human herpesvirus 6A infects human embryonic fibroblasts and induces G2/M arrest and cell death.
abstract: Human herpesvirus 6 (HHV-6) is a beta-herpesvirus capable of infecting cells from different origin. In this study, infection with HHV-6A of human embryonic fibroblasts (HEFs) was performed. Infected cells showed obvious cytopathic effects (CPE). PCR and immunohistochemical tests also confirmed that HEFs are susceptible to HHV-6A infection. The biological effects of HHV-6A infection on HEFs were studied. Infected cells showed decreased proliferation as measured by [(3)H] thymidine incorporation and cell counting. Further analysis demonstrated that infection with HHV-6A leads to cell cycle arrest at G2/M phase and increasing cell death. This is the first demonstration that infection of HEFs with HHV-6A causes profound alterations of cell properties.
authors.affiliation: Department of Microbiology and Immunology, Nanjing Medical University, Nanjing, China.
authors.forename: Lingyun
authors.initials: L
authors.lastname: Li
authors.forename: Bin
authors.initials: B
authors.lastname: Gu
authors.forename: Feng
authors.initials: F
authors.lastname: Zhou
authors.forename: Jing
authors.initials: J
authors.lastname: Chi
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Genyan
authors.initials: G
authors.lastname: Liu
authors.forename: Chuanlin
authors.initials: C
authors.lastname: Ding
authors.forename: Fangyi
authors.initials: F
authors.lastname: Xie
authors.forename: Jian
authors.initials: J
authors.lastname: Qing
authors.forename: Yidi
authors.initials: Y
authors.lastname: Guo
authors.forename: Kun
authors.initials: K
authors.lastname: Yao
mesh_terms: D016923:Cell Death; D002455:Cell Division; D049109:Cell Proliferation; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D005347:Fibroblasts / Q000473:pathology / Q000821:virology*; D015654:Herpesvirus 6, Human / Q000472:pathogenicity*; D006801:Humans; D007150:Immunohistochemistry; D016133:Polymerase Chain Reaction