title: [Expression of hSef and FGF-2 in epithelial ovarian tumor].
abstract: OBJECTIVE
  To detect the expression of human similar expression to FGF gene(hSef) and fibroblast growth factor-2(FGF-2) and their correlation with epithelial ovarian tumor.
  
  
  METHODS
  Immunohistochemical SP staining was used to detect the expression of hSef and FGF-2 proteins in 31 cases of epithelial ovarian carcinoma (EOC), 18 cases of benign epithelial tumor (BET), 10 cases of normal ovarian (NO) tissues collected from July 2007 to May 2008. The expression of hSef mRNA in 24 cases of EOC, BET and NO collected from July 2008 to May 2009 were analyzed by RT-PCR.
  
  
  RESULTS
  The results of immunohistochemical study showed that the expression of hSef in the EOC tissues were significantly lower than that in the NO and BET (P < 0.001). However, the expression of FGF-2 was higher (P = 0.002). The expression of hSef had a negative correlation with FGF-2 (r(s) = -0.324, P = 0.012). The RT-PCR results showed that there was a gradually declined trend of expression of hSef in NO, BET to EOC (P < 0.001), but the expression of FGF-2 in NO, BET to EOC was gradually increased (P < 0.001), with a significant negative correlation (NO: r(s) = -0.910, P < 0.001; BET: r(s) = -0.859, P < 0.001; EOC: r(s) = -0.888, P < 0.001).
  
  
  CONCLUSIONS
  The expression of hSef is decreased in epithelial ovarian carcinoma tissue, but the expression of FGF-2 is increased. It is likely that low hSef expression is related to the the carcinogenesis and development of epithelial ovarian carcinoma by suppressing the promoting effects of FGF-2 to cell proliferation.
authors.affiliation: Department of Gynecology, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Quan-ling
authors.initials: QL
authors.lastname: Feng
authors.forename: Hui-rong
authors.initials: HR
authors.lastname: Shi
authors.forename: Li-juan
authors.initials: LJ
authors.lastname: Qiao
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
mesh_terms: D000328:Adult; D000368:Aged; D018282:Cystadenocarcinoma, Mucinous / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D018284:Cystadenocarcinoma, Serous / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D018291:Cystadenoma, Mucinous / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D018293:Cystadenoma, Serous / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000235:genetics / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D010053:Ovary / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D018123:Receptors, Interleukin / Q000235:genetics / Q000378:metabolism*
chemical_list: C477526:IL17RD protein, human; D012333:RNA, Messenger; D018123:Receptors, Interleukin; D016222:Fibroblast Growth Factor 2