title: The roles of RGD and grooved topography in the adhesion, morphology, and differentiation of C2C12 skeletal myoblasts.
abstract: Both chemical and topographic cues are crucial for the development of skeletal muscle. In this study, the relative roles of both signals in regard to cell adhesion, morphology, and differentiation of C2C12 skeletal myoblasts were investigated. Grooved polystyrene substrates containing grooves with approximately 900 nm in width with 600 nm ridge spans and 665 nm in depth were conjugated with the cell adhesion peptide arginine-glycine-aspartic acid (RGD). RGD conjugation significantly enhanced the adhesion, growth and differentiation of C2C12 cells. On the other hand, anisotropic topography primarily directed the direction and alignment of myoblasts and myotubes. The results in this study provide information regarding the relative roles of chemical and topographic cues in musculoskeletal myogenesis, and are of interest to applications in muscle tissue engineering.
authors.affiliation: Department of Chemical Engineering, National Taiwan University, No. 1, Roosevelt Rd., Sec. 4, Taipei 106, Taiwan.
authors.forename: Peng-Yuan
authors.initials: PY
authors.lastname: Wang
authors.forename: Helmut
authors.initials: H
authors.lastname: Thissen
authors.forename: Wei-Bor
authors.initials: WB
authors.lastname: Tsai
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D051379:Mice; D032448:Myoblasts, Skeletal / Q000166:cytology* / Q000502:physiology*; D009842:Oligopeptides / Q000378:metabolism*; D013499:Surface Properties
chemical_list: D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid