title: Intrinsic regenerative potential of murine cochlear supporting cells.
abstract: The lack of cochlear regenerative potential is the main cause for the permanence of hearing loss. Albeit quiescent in vivo, dissociated non-sensory cells from the neonatal cochlea proliferate and show ability to generate hair cell-like cells in vitro. Only a few non-sensory cell-derived colonies, however, give rise to hair cell-like cells, suggesting that sensory progenitor cells are a subpopulation of proliferating non-sensory cells. Here we purify from the neonatal mouse cochlea four different non-sensory cell populations by fluorescence-activated cell sorting (FACS). All four populations displayed proliferative potential, but only lesser epithelial ridge and supporting cells robustly gave rise to hair cell marker-positive cells. These results suggest that cochlear supporting cells and cells of the lesser epithelial ridge show robust potential to de-differentiate into prosensory cells that proliferate and undergo differentiation in similar fashion to native prosensory cells of the developing inner ear.
authors.affiliation: Department of Otolaryngology - Head & Neck Surgery, Stanford University School of Medicine, Stanford CA 94305, USA.
authors.forename: Saku T
authors.initials: ST
authors.lastname: Sinkkonen
authors.forename: Renjie
authors.initials: R
authors.lastname: Chai
authors.forename: Taha A
authors.initials: TA
authors.lastname: Jan
authors.forename: Byron H
authors.initials: BH
authors.lastname: Hartman
authors.forename: Roman D
authors.initials: RD
authors.lastname: Laske
authors.forename: Felix
authors.initials: F
authors.lastname: Gahlen
authors.forename: Wera
authors.initials: W
authors.lastname: Sinkkonen
authors.forename: Alan G
authors.initials: AG
authors.lastname: Cheng
authors.forename: Kazuo
authors.initials: K
authors.lastname: Oshima
authors.forename: Stefan
authors.initials: S
authors.lastname: Heller
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015703:Antigens, CD / Q000276:immunology; D002469:Cell Separation; D003051:Cochlea / Q000166:cytology*; D005434:Flow Cytometry; D049452:Green Fluorescent Proteins / Q000235:genetics; D007150:Immunohistochemistry; D016130:Immunophenotyping; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008855:Microscopy, Electron, Scanning; D012038:Regeneration
chemical_list: D015703:Antigens, CD; D049452:Green Fluorescent Proteins