title: A new apparatus for studying the effect of hydrostatic pressure on cells in culture : application to osteoblastic cells ROS 17/2.8.
abstract: Although mechanical stresses have long been recognized as an important factor in the regulation of bone remodeling, the mechanism underlying this effect has remained obscure. A number of methods have been devised to apply forces to bone tissues and bone-derived cells in order to investigate the biochemical results of mechanical stimuli. In this paper we report a method for applying a well controlled cyclic hydrostatic pressure on cultured ROS 17/2.8 osteoblastic lineage cells. This technique allows the investigation of the true frequency response of cells. Hydrostatic pressure with a 1 Hz frequency decreases alkaline phosphatase activity of confluent osteoblastic-like cells (ROS 17/2.8).
authors.affiliation: Laboratoire de Recherches Orthopédiques, Université Paris VII Denis Diderot, URA CNRS 1432, Faculté de Médecine Lariboisière Saint-Louis, 10 Avenue de Verdun, 75010, Paris, France.
authors.forename: L
authors.initials: L
authors.lastname: Vergne
authors.forename: A
authors.initials: A
authors.lastname: Meunier
authors.forename: M
authors.initials: M
authors.lastname: Adolphe
authors.forename: L
authors.initials: L
authors.lastname: Sedel