title: Peroxidase-like activity of water-soluble cupric oxide nanoparticles and its analytical application for detection of hydrogen peroxide and glucose.
abstract: Water-soluble cupric oxide nanoparticles are fabricated via a quick-precipitation method and used as peroxidase mimetics for ultrasensitive detection of hydrogen peroxide and glucose. The water-soluble CuO nanoparticles show much higher catalytic activity than that of commercial CuO nanoparticles due to their higher affinity to hydrogen peroxide. In addition, the as-prepared CuO nanoparticles are stable over a wide range of pH and temperature. This excellent stability in the form of aqueous colloidal suspensions makes the application of the water-soluble CuO nanoparticles easier in aqueous systems. A colorimetric assay for hydrogen peroxide and glucose has been established based on the catalytic oxidation of phenol coupled with 4-amino-atipyrine by the action of hydrogen peroxide. This analytical platform not only confirms the intrinsic peroxidase-like activity of the water-soluble cupric oxide nanoparticles, but also shows its great potential applications in environmental chemistry, biotechnology and medicine.
authors.affiliation: Department of Pharmaceutical Analysis, Fujian Medical University, Fuzhou 350004, China. chenandhu@163.com
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Juan
authors.initials: J
authors.lastname: Chen
authors.forename: Ye-Bin
authors.initials: YB
authors.lastname: Feng
authors.forename: Lei
authors.initials: L
authors.lastname: Hong
authors.forename: Qi-Ying
authors.initials: QY
authors.lastname: Chen
authors.forename: Ling-Feng
authors.initials: LF
authors.lastname: Wu
authors.forename: Xin-Hua
authors.initials: XH
authors.lastname: Lin
authors.forename: Xing-Hua
authors.initials: XH
authors.lastname: Xia
mesh_terms: D003124:Colorimetry; D003300:Copper / Q000737:chemistry*; D005947:Glucose / Q000032:analysis*; D006861:Hydrogen Peroxide / Q000032:analysis*; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D009195:Peroxidase / Q000737:chemistry*; D012995:Solubility; D014867:Water / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D014867:Water; D003300:Copper; D006861:Hydrogen Peroxide; D009195:Peroxidase; D005947:Glucose; C030973:cupric oxide