title: Comparative cytogenetic mapping between the lima bean (Phaseolus lunatus L.) and the common bean (P. vulgaris L.).
abstract: The common bean (Phaseolus vulgaris) and lima bean (P. lunatus) are among the most important legumes in terms of direct human consumption. The present work establishes a comparative cytogenetic map of P. lunatus, using previously mapped markers from P. vulgaris, in association with analyses of heterochromatin distribution using the fluorochromes chromomycin A3 (CMA) and 4',6-diamidino-2-phenylindole (DAPI) and localization of the 5S and 45S ribosomal DNA (rDNA) probes. Seven BACs selected from different common bean chromosomes demonstrated a repetitive pericentromeric pattern corresponding to the heterochromatic regions revealed by CMA/DAPI and could not be mapped. The subtelomeric repetitive pattern observed for BAC 63H6 in most of the chromosome ends of common bean was not detected in lima bean, indicating lack of conservation of this subtelomeric repeat. All chromosomes could be identified and 16 single-copy clones were mapped. These results showed a significant conservation of synteny between species, although change in centromere position suggested the occurrence of pericentric inversions on chromosomes 2, 9 and 10. The low number of structural rearrangements reflects the karyotypic stability of the genus.
authors.affiliation: Laboratory of Plant Cytogenetics, Department of Botany, Federal University of Pernambuco, Rua Nelson Chaves, Recife, PE 50670-420, Brazil.
authors.forename: Eliene Mariano
authors.initials: EM
authors.lastname: Bonifácio
authors.forename: Artur
authors.initials: A
authors.lastname: Fonsêca
authors.forename: Cícero
authors.initials: C
authors.lastname: Almeida
authors.forename: Karla G B
authors.initials: KG
authors.lastname: Dos Santos
authors.forename: Andrea
authors.initials: A
authors.lastname: Pedrosa-Harand
mesh_terms: D002503:Centromere / Q000648:ultrastructure; D014128:Chromomycin A3 / Q000378:metabolism; D002874:Chromosome Mapping / Q000379:methods; D002875:Chromosomes / Q000648:ultrastructure; D003582:Cytogenetics; D004275:DNA, Ribosomal / Q000235:genetics; D007887:Fabaceae / Q000235:genetics*; D017343:Genes, Plant; D006570:Heterochromatin / Q000235:genetics / Q000378:metabolism; D017404:In Situ Hybridization, Fluorescence; D007211:Indoles / Q000737:chemistry; D007621:Karyotyping; D008957:Models, Genetic; D027805:Phaseolus / Q000235:genetics*; D016615:Telomere / Q000648:ultrastructure
chemical_list: D004275:DNA, Ribosomal; D006570:Heterochromatin; D007211:Indoles; C007293:DAPI; D014128:Chromomycin A3