title: A high-efficiency Agrobacterium-mediated transformation system of rice (Oryza sativa L.).
abstract: Agrobacterium-mediated transformation of rice has been routinely performed according to the protocol reported by Hiei et al. (Plant J. 6:271-282, 1994). However, several elite japonica and many indica varieties cannot be efficiently transformed by Agrobacterium system. Also a large number of transformants are required to generate T-DNA insertion and FOX libraries as well as gene-targeting studies. To overcome these challenges, we established a high-efficiency transformation system in rice by cocultivating rice calli with Agrobacterium on filter papers moistened with enriched liquid media instead of using solid media (Ozawa, Plant Sci. 176:522-527, 2009; Ozawa and Takaiwa, Plant Sci. 179:333-337, 2010). In this system, the transformation efficiency of the calli is almost 100% in many varieties.
authors.affiliation: Transgenic Crop Research and Development Center, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan. ozawa@affrc.go.jp
authors.forename: Kenjirou
authors.initials: K
authors.lastname: Ozawa
mesh_terms: D060054:Agrobacterium / Q000235:genetics*; D018920:Coculture Techniques / Q000379:methods; D004269:DNA, Bacterial / Q000235:genetics*; D018014:Gene Transfer Techniques; D012275:Oryza / Q000235:genetics*; D030821:Plants, Genetically Modified; D046509:Tissue Culture Techniques / Q000379:methods; D014170:Transformation, Genetic
chemical_list: D004269:DNA, Bacterial; C036483:T-DNA