title: Study on ITS1 Gene of Iranian Trichomonas vaginalis by Molecular Methods.
abstract: BACKGROUND
  Trichomoniasis is a worldwide protozoan parasitic disease and metronidazole is a choice drug for its treatment. Because of disease importance in public health and its controversial ideas about the prevalence of drug resistance, this study was carried out.
  
  
  METHODS
  Fifty-two suspected vaginal samples were collected from 2006 to 2007 in Gynecology Maryam Hospital, Tehran, Iran. All isolates were examined by microscopic, culture and PCR techniques. The PCR products were analyzed by RFLP and CSGE methods and two suspected samples were sequenced.
  
  
  RESULTS
  Trichomonas vaginalis was identified from all 52 samples. Of 52 isolates, 45 samples were successfully cultured and amplified by PCR except one. Seven were positive only by PCR. Finally, ITS1 fragment was successfully amplified in 51 of 52. CSGE analysis and PCR products digestion by MspI followed by sequencing showed nucleotide mutation at position 209 (C209T) of the ITS1 fragment in two (3.9%) of them.
  
  
  CONCLUSIONS
  The results showed mutation in ITS1 fragment of T. vaginalis in two (3.9%) of Iranian isolates which may be related to metronidazole resistance.
authors.affiliation: Parasitology Dept., School of Medicine, Kashan University of Medical Sciences, Kashan, Iran.
authors.forename: F
authors.initials: F
authors.lastname: Kazemi
authors.forename: H
authors.initials: H
authors.lastname: Hooshyar
authors.forename: B
authors.initials: B
authors.lastname: Zareikar
authors.forename: M
authors.initials: M
authors.lastname: Bandehpour
authors.forename: M
authors.initials: M
authors.lastname: Arbabi
authors.forename: S
authors.initials: S
authors.lastname: Talari
authors.forename: R
authors.initials: R
authors.lastname: Alizadeh
authors.forename: B
authors.initials: B
authors.lastname: Kazemi