title: Influence of fatty acids in maternal diet on atherogenesis in offspring of LDL receptor-deficient mice.
abstract: OBJECTIVE
  The present study investigated the effect of a maternal diet rich in omega-6 (E6D) or omega-9 (E9D) on atherogenesis in the offspring of mice.
  
  
  METHODS
  LDL receptor-deficient mice were fed a diet rich in either omega-6 (E6D) or omega-9 (E9D) for 45 days prior to mating and until the birth of the offspring, evaluating the effect on the offspring aorta in comparison to a standard diet (STD), by immunohistochemical analysis, morphometric analysis and electron microscopy.
  
  
  RESULTS
  Hypercholesterolemic female mice fed E6D generated offspring with high levels of total cholesterol, triglycerides (TG) and CC-chemokine ligand 2/monocyte chemoattractant protein 1 (CCL2/ MCP-1) as well as a reduction in high-density lipoprotein. The ascending aorta of these animals exhibited an increase in arterial wall thickness as well as increased expression of CCL2/MCP-1 and vascular cell adhesion molecule 1. The ultrastructural analysis revealed severe alterations in endothelial cells. The offspring from mothers fed E9D exhibited a reduction in TG and an increase in low-density lipoprotein. The ultrastructural analysis revealed a well-preserved aortic endothelium in these animals.
  
  
  CONCLUSIONS
  The results suggest that hypercholesterolemic mothers feed a diet rich in omega-6 predispose their offspring to endothelial dysfunction.
authors.forename: Dilênia
authors.initials: D
authors.lastname: de Oliveira Cipriano Torres
authors.forename: Ana Célia Oliveira Dos
authors.initials: AC
authors.lastname: Santos
authors.forename: Amanda Karolina Soares E
authors.initials: AK
authors.lastname: Silva
authors.forename: Patrícia Muniz Mendes Freire
authors.initials: PM
authors.lastname: de Moura
authors.forename: Eduardo Isidoro Carneiro
authors.initials: EI
authors.lastname: Beltrão
authors.forename: Christina Alves
authors.initials: CA
authors.lastname: Peixoto