title: Pik1-ing clathrin adaptors.
abstract: Clathrin adaptor proteins are essential for clathrin-coated vesicle biogenesis, yet the mechanisms governing their recruitment and interactions remain incompletely defined. The clathrin adaptors Gga and AP-1 are now shown to be recruited sequentially to the trans-Golgi network in two waves of clathrin coat assembly, coupled by Pik1-mediated phosphatidylinositol-4-phosphate synthesis. These findings reveal mechanistic insights into the functional and regulatory relationships between these clathrin adaptors.
authors.affiliation: Department of Molecular and Cell Biology, University of California Berkeley, California 94720-3202, USA.
authors.forename: Yidi
authors.initials: Y
authors.lastname: Sun
authors.forename: David G
authors.initials: DG
authors.lastname: Drubin
mesh_terms: D019870:1-Phosphatidylinositol 4-Kinase / Q000378:metabolism*; D033942:Adaptor Proteins, Vesicular Transport / Q000378:metabolism*; D018129:Phosphatidylinositol Phosphates / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D021601:trans-Golgi Network / Q000378:metabolism*
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; C497361:Gga2 protein, S cerevisiae; D018129:Phosphatidylinositol Phosphates; D029701:Saccharomyces cerevisiae Proteins; C037178:phosphatidylinositol 4-phosphate; D019870:1-Phosphatidylinositol 4-Kinase; C441760:PIK1 protein, S cerevisiae