title: [Screening of serum marker proteins in osteosarcoma and preliminary bioinformatic analysis on POLR3F].
abstract: OBJECTIVE
  To screen potential serum marker proteins of osteosarcoma, and to make a preliminary bioinformatics analysis of RNA polymerase III polypeptide F (POLR3F).
  
  
  METHODS
  Gene chip and SELDI-TOF MS was used to screen genes differentially expressed in osteosarcoma. The associations of potential biomarkers from SELDI data and microarray analysis were further inferred by link-test to identify biomarkers that could likely be used for diagnosis. MATLAB was used to search transcription factors binding site in the promoter region and miRNAs binding site in 3'-UTR of POLR3F, respectively.
  
  
  RESULTS
  653 differentially expressed genes were found in osteosarcoma cells, while six differentially expressed protein peaks with significant statistical significances were detected by SELDI-TOF MS in patient's serum. 13 potential biomarkers for early diagnosis of osteosarcoma were screened by link-test. A conserved STAT3 binding site and a miRNA target site were found in proximal promoter regions and 3'-UTR region of POLR3F, respectively.
  
  
  CONCLUSIONS
  Link-test is a effective method to identify osteosarcoma biomarkers from both microarray and SELDI-TOF MS database. The results confirmed that POLR3F may be a promising biomarker for early diagnosis and a therapy target of osteosarcoma.
authors.affiliation: Department of Orthopaedics, Shanghai Tenth People's Hospital, Shanghai, China. litrue2004@yahoo.com.cn
authors.forename: Guo-dong
authors.initials: GD
authors.lastname: Li
authors.forename: Zheng-dong
authors.initials: ZD
authors.lastname: Cai
authors.forename: Xiao-jun
authors.initials: XJ
authors.lastname: Ma
authors.forename: Meng-xiong
authors.initials: MX
authors.lastname: Sun
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.forename: Ying-qi
authors.initials: YQ
authors.lastname: Hua
mesh_terms: D000293:Adolescent; D000328:Adult; D001665:Binding Sites; D014408:Biomarkers, Tumor / Q000097:blood*; D001798:Blood Proteins / Q000032:analysis; D001859:Bone Neoplasms / Q000235:genetics / Q000378:metabolism*; D002648:Child; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D012516:Osteosarcoma / Q000235:genetics / Q000378:metabolism*; D010455:Peptides / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D012320:RNA Polymerase III / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D050796:STAT3 Transcription Factor / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D001798:Blood Proteins; D010455:Peptides; D021122:Protein Subunits; D012333:RNA, Messenger; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D012320:RNA Polymerase III