title: Use of multiphoton microscopy to diagnose liver cancer and lung metastasis in an orthotopic rat model.
abstract: Liver or lung biopsy for suspicious lesions has several disadvantages such as bleeding, bile leak or pneumothorax, needle track seeding, and time-consuming histopathological procedure. The ability to directly observe cellular and subcellular details and then perform "optical biopsy" is a major goal in the development of new interventional techniques. Multiphoton microscopy (MPM) enables real-time noninvasive visualization of tissue architecture and cell morphology in live tissue. We performed a study to evaluate whether MPMcan make real-time optical diagnosis for liver cancer and lung metastasis using an orthotopic rat model with Morris hepatoma. We found that real-time high-resolution MPMimaging could clearly show tissue architecture and cell morphology. In the normal liver tissue, MPMimaging clearly revealed the blood-filled sinusoids and cords of hepatocytes. In the cancerous tissue, MPMimaging clearly illustrated that cancer cells displayed marked cellular and nuclear pleomorphism. MPMimages were comparable to golden standard hematoxylin-eosin staining images. Moreover, MPMimaging had deep penetration with the capability of optical sectioning. In short, MPMcan make real-time optical diagnosis for liver cancer and lung metastasis. This study provides the groundwork for further using multiphoton endoscopy to perform real-time noninvasive "optical biopsy" for liver cancer and lung metastasis in the near future.
authors.affiliation: Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yan
authors.forename: Shuangmu
authors.initials: S
authors.lastname: Zhuo
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
authors.forename: Changjun
authors.initials: C
authors.lastname: Tan
authors.forename: Weifeng
authors.initials: W
authors.lastname: Zhu
authors.forename: Jianping
authors.initials: J
authors.lastname: Lu
authors.forename: Jia
authors.initials: J
authors.lastname: Fan
authors.forename: Jianxin
authors.initials: J
authors.lastname: Chen
authors.forename: Jian
authors.initials: J
authors.lastname: Zhou
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D007091:Image Processing, Computer-Assisted / Q000379:methods; D008113:Liver Neoplasms / Q000150:complications / Q000175:diagnosis* / Q000473:pathology; D008114:Liver Neoplasms, Experimental; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000473:pathology / Q000556:secondary; D008853:Microscopy / Q000379:methods*; D009362:Neoplasm Metastasis / Q000175:diagnosis* / Q000473:pathology; D051381:Rats