title: [Genetic manipulation and studying of differentiation properties of rat induced pluripotent stem cells].
abstract: Induced pluripotent stem (iPS) cells are derived from somatic cells reprogrammed to the pluripotent state by the induced expression of defined transcription factors, achieved for the first time by the seminal work of Takahashi and Yamanaka. This new type of pluripotent cells has offered new exciting options in regenerative medicine allowing the replacement of cells and organs with the patient's own cells thereby avoiding immunological complications. In order to develop such technologies in approved animal models, iPS cells were also generated from rodents. Of course, the most important model for studying of different diseases is rat. In this study, we present a method suitable for rat iPS cells genetic modification by stable transfection and show necessary conditions for the first stages of direct differentiation.
authors.forename: M A
authors.initials: MA
authors.lastname: Liskovykh
authors.forename: I A
authors.initials: IA
authors.lastname: Chuĭkin
authors.forename: A
authors.initials: A
authors.lastname: Ranjan
authors.forename: E
authors.initials: E
authors.lastname: Popova
authors.forename: E N
authors.initials: EN
authors.lastname: Tolkunova
authors.forename: L L
authors.initials: LL
authors.lastname: Chechik
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Malinin
authors.forename: A V
authors.initials: AV
authors.lastname: Morozova
authors.forename: V
authors.initials: V
authors.lastname: Mosienko
authors.forename: M
authors.initials: M
authors.lastname: Bader
authors.forename: N
authors.initials: N
authors.lastname: alenina
authors.forename: A N
authors.initials: AN
authors.lastname: Tomilin
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D002460:Cell Line; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D051381:Rats; D044968:Regenerative Medicine / Q000379:methods; D014162:Transfection / Q000379:methods