title: Human chorionic gonadotropin suppresses expression of Piwis in common carp (Cyprinus carpio) ovaries.
abstract: Piwi proteins are required for germline maintenance and gonad development. In this study, the cDNAs encoding Piwil1 and Piwil2 were cloned and sequenced from the common carp. The full-length cDNA of Piwil1 and Piwil2 were 3114 and 3421bp, encoding 858 and 1034 amino acids including PAZ domain and PIWI domain, respectively. In addition, the Piwil1 and Piwil2 proteins shared high homology with other teleosts. Reverse transcriptase PCR revealed that the Piwi mRNAs were exclusively expressed in adult testes and ovaries. Using real-time PCR, expression study of different developmental profiles showed that Piwil1 and Piwil2 were down-regulated during pre-ovulation. Further, human chorionic gonadotropin treatment in ovaries (in vivo) and in cultured ovaries cells (in vitro) resulted in down-regulation of Piwi RNAs. These results suggest that the decreased expression which was regulated by hormone plays a crucial role during ovarian differentiation and development.
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of State Education Ministry of China, College of Life Sciences, Hunan Normal University, ChangSha 410081, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhou
authors.forename: Fenghua
authors.initials: F
authors.lastname: Wang
authors.forename: Shaojun
authors.initials: S
authors.lastname: Liu
authors.forename: Huan
authors.initials: H
authors.lastname: Zhong
authors.forename: Zhen
authors.initials: Z
authors.lastname: Liu
authors.forename: Min
authors.initials: M
authors.lastname: Tao
authors.forename: Chun
authors.initials: C
authors.lastname: Zhang
authors.forename: Yun
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000818:Animals; D060565:Argonaute Proteins / Q000235:genetics*; D002347:Carps; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D005260:Female; D029941:Fish Proteins / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000235:genetics; D006801:Humans; D008297:Male; D010053:Ovary / Q000187:drug effects / Q000378:metabolism*; D010802:Phylogeny; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D060565:Argonaute Proteins; D006063:Chorionic Gonadotropin; D029941:Fish Proteins