title: Evaluation of the Xpert Flu test and comparison with in-house real-time RT-PCR assays for detection of influenza virus from 2008 to 2011 in Marseille, France.
abstract: Rapid documentation of respiratory specimens can have an impact on the management of patients and their relatives in terms of preventive and curative measures. We compared the results of the Xpert(®) Flu assay (Cepheid) with three real-time RT-PCR assays using 127 nasopharyngeal samples, of which 75 were positive for influenza A (with 52 identified as A/H1N1-2009) and 52 were positive for influenza B. The Xpert(®) Flu assay presented a quasi-absence of non-interpretable tests, and showed sensitivity and specificity of 100% and 100% for Flu A, 98.4% and 100% for A/H1N1-2009, and 80.7% and 100% for Flu B.
authors.affiliation: UMR190 'Emergence des Pathologies Virales'(Aix-Marseille Univ - Institute of Research for Development - EHESP French School of Public Health), Marseille, France ) Federation of Clinical Microbiology, AP-HM Timone, Marseille, France.
authors.forename: N
authors.initials: N
authors.lastname: Salez
authors.forename: L
authors.initials: L
authors.lastname: Ninove
authors.forename: L
authors.initials: L
authors.lastname: Thirion
authors.forename: C
authors.initials: C
authors.lastname: Gazin
authors.forename: C
authors.initials: C
authors.lastname: Zandotti
authors.forename: X
authors.initials: X
authors.lastname: de Lamballerie
authors.forename: R N
authors.initials: RN
authors.lastname: Charrel
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D005260:Female; D005602:France / Q000453:epidemiology; D006801:Humans; D007223:Infant; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D009981:Influenza B virus / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D007251:Influenza, Human / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D008297:Male; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009305:Nasopharynx / Q000821:virology; D012367:RNA, Viral / Q000235:genetics; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D012744:Sex Ratio; D055815:Young Adult
chemical_list: D012367:RNA, Viral