title: Distinct volume distribution of viable and non-viable hybridoma cells: A flow cytometric study.
abstract: Light scattering properties of hybridoma cells were examined with flow cytometry. Viable and dead cells form two distinct populations. The distribution of the two populations changes during a batch culture. the concentration of dead cells measured by flow cytometry correlates well to that measured by hemacytometer. The distribution based on small-angle light scattering is similar to the distribution based on volume as measured by Elzone particle counter. It thus appears that viable cells form the population with a larger mean cell volume. The results also indicate that the volume of viable cells decreases during the cultivation while that of dead cells remains relatively constant.
authors.affiliation: Department of Chemical Engineering and Materials Science, University of Minnesota, 55455, Minneapolis, MN, USA.
authors.forename: S
authors.initials: S
authors.lastname: Sen
authors.forename: F
authors.initials: F
authors.lastname: Srienc
authors.forename: W S
authors.initials: WS
authors.lastname: Hu