title: Split-transgene expression in wheat.
abstract: The establishment of traits that result from the concerted expression of complementing transgene fragments is a feasible tool for trait control or gene flow control in plants. This chapter describes the methodology for producing herbicide-resistant and pollen-sterile wheat plants by the intein-mediated assembly of inactive precursor protein fragments (protein trans-splicing). We suggest the design of intein-containing vectors for split-transgene expression. We describe transient plant assays that can be used to analyse the functionality of the system and describe the transformation of wheat plants using a split selection marker.We hope that this chapter will be a helpful guideline for researchers who are interested in applying similar split-gene approaches in wheat or other monocotyledonous crops.
authors.affiliation: Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK) Gatersleben, Gatersleben, Germany. gils@ipk-gatersleben.de
authors.forename: Mario
authors.initials: M
authors.lastname: Gils
authors.forename: Myroslava
authors.initials: M
authors.lastname: Rubtsova
authors.forename: Katja
authors.initials: K
authors.lastname: Kempe
mesh_terms: D000095:Acetolactate Synthase / Q000235:genetics*; D001426:Bacterial Proteins; D005822:Genetic Vectors; D053209:Herbicide Resistance / Q000235:genetics*; D006540:Herbicides / Q000494:pharmacology; D047668:Inteins / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics; D011058:Pollen / Q000235:genetics / Q000502:physiology; D019154:Protein Splicing; D012260:Ribonucleases / Q000096:biosynthesis / Q000235:genetics; D046939:Synechocystis / Q000235:genetics; D014026:Nicotiana / Q000235:genetics; D020040:Trans-Splicing; D019076:Transgenes; D014908:Triticum / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D006540:Herbicides; D000095:Acetolactate Synthase; D012260:Ribonucleases; C019760:Bacillus amyloliquefaciens ribonuclease