title: Characterization of a neutral pectin lyase produced by Oidiodendron echinulatum MTCC 1356 in solid state fermentation.
abstract: A neutral pectin lyase produced by a new fungal strain Oidiodendron echinulatum MTCC 1356 under solid state fermentation using wheat bran as agro waste has been studied. The enzyme was purified by ammonium sulphate precipitation (30-60%), DEAE anion exchange and Sephadex G-100 column chromatographies. The SDS-PAGE and native PAGE revealed two bands of sizes 42 and 47 kDa. The enzyme was purified 37 fold with specific activity of 4.5 U/mg and 2.25% yield. The K(m) and V(max) values determined using citrus pectin were 1.2 mg/ml and 0.36 IU/min respectively. The pH and temperature optima were pH 7.0 and 50 °C, respectively. The pH stability was around 5.0 for 24 h at 20 °C. The purified enzyme retained maximum activity for 30 min upto 50 °C. The activation energy for thermal denaturation of the purified enzyme was found to be 60.0 kJ/Mol. The effects of various metal ions and protein inhibitors on enzyme activity have revealed total inhibition of the enzyme activity in the presence of Ag(+) and Cu(+) and KMnO(4) at 1 mM. The neutral pectin lyase showed retting of Crotalaria juncea fibre in the presence of EDTA.
authors.affiliation: Department of Biotechnology, D.D.U. Gorakhpur University, Gorakhpur U.P., India.
authors.forename: Sangeeta
authors.initials: S
authors.lastname: Yadav
authors.forename: Amit Kumar
authors.initials: AK
authors.lastname: Dubey
authors.forename: Gautam
authors.initials: G
authors.lastname: Anand
authors.forename: Dinesh
authors.initials: D
authors.lastname: Yadav
mesh_terms: D005285:Fermentation; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008670:Metals / Q000494:pharmacology; D011133:Polysaccharide-Lyases / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D004718:Saccharomycetales / Q000187:drug effects / Q000201:enzymology*; D013696:Temperature
chemical_list: D005656:Fungal Proteins; D008670:Metals; D011133:Polysaccharide-Lyases; C027349:pectin lyase