title: Bone marrow-derived IL-13Rα1-positive thymic progenitors are restricted to the myeloid lineage.
abstract: The earliest thymic progenitors (ETPs) were recently shown to give rise to both lymphoid and myeloid cells. Whereas the majority of ETPs are derived from IL-7Rα-positive cells and give rise exclusively to T cells, the origin of the myeloid cells remains undefined. In this study, we show both in vitro and in vivo that IL-13Rα1(+) ETPs yield myeloid cells with no potential for maturation into T cells, whereas IL-13Rα1(-) ETPs lack myeloid potential. Moreover, transfer of lineage-negative IL-13Rα1(+) bone marrow stem cells into IL-13Rα1-deficient mice reconstituted thymic IL-13Rα1(+) myeloid ETPs. Myeloid cells or macrophages in the thymus are regarded as phagocytic cells whose function is to clear apoptotic debris generated during T cell development. However, the myeloid cells derived from IL-13Rα1(+) ETPs were found to perform Ag-presenting functions. Thus, IL-13Rα1 defines a new class of myeloid restricted ETPs yielding APCs that could contribute to development of T cells and the control of immunity and autoimmunity.
authors.affiliation: Department of Molecular Microbiology and Immunology, University of Missouri School of Medicine, Columbia, MO 65212, USA.
authors.forename: Cara L
authors.initials: CL
authors.lastname: Haymaker
authors.forename: F Betul
authors.initials: FB
authors.lastname: Guloglu
authors.forename: Jason A
authors.initials: JA
authors.lastname: Cascio
authors.forename: John C
authors.initials: JC
authors.lastname: Hardaway
authors.forename: Mermagya
authors.initials: M
authors.lastname: Dhakal
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Wan
authors.forename: Christine M
authors.initials: CM
authors.lastname: Hoeman
authors.forename: Sarah
authors.initials: S
authors.lastname: Zaghouani
authors.forename: Linda M
authors.initials: LM
authors.lastname: Rowland
authors.forename: Danielle M
authors.initials: DM
authors.lastname: Tartar
authors.forename: Amie M
authors.initials: AM
authors.lastname: VanMorlan
authors.forename: Habib
authors.initials: H
authors.lastname: Zaghouani
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000737:chemistry / Q000166:cytology* / Q000187:drug effects / Q000276:immunology; D000943:Antigens, Differentiation / Q000032:analysis*; D001854:Bone Marrow Cells / Q000737:chemistry / Q000145:classification*; D019070:Cell Lineage; D002465:Cell Movement; D002478:Cells, Cultured; D005260:Female; D055879:Gene Knock-In Techniques; D055014:Granulocyte-Macrophage Progenitor Cells / Q000737:chemistry / Q000166:cytology* / Q000187:drug effects / Q000276:immunology; D018793:Interleukin-13 / Q000494:pharmacology; D053661:Interleukin-13 Receptor alpha1 Subunit / Q000032:analysis* / Q000172:deficiency / Q000235:genetics; D008215:Lymphocytes, Null / Q000166:cytology; D038041:Lymphopoiesis; D008297:Male; D051379:Mice; D020297:Mice, Congenic; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D038042:Myelopoiesis; D017384:Sequence Deletion; D013601:T-Lymphocytes / Q000166:cytology; D013950:Thymus Gland / Q000166:cytology*
chemical_list: D000943:Antigens, Differentiation; C508183:Il13ra1 protein, mouse; D018793:Interleukin-13; D053661:Interleukin-13 Receptor alpha1 Subunit