title: Lipopolysaccharide interference in erythropoiesis in mice.
abstract: Anaemia is a common problem in septic patients. We tested whether lipopolysaccharide suppressed erythropoiesis and interfered with erythropoietin. Male mice (strain C57BL/6, n = 76) were injected Escherichia coli lipopolysaccharide (serotype O127:B8; 20 mg.kg(-1) intraperitoneally) or vehicle, followed by either erythropoietin (5000 IU.kg(-1) intraperitoneally) or vehicle, and killed after 24 or 72 h. Femur bone marrow cells were stained for Ter-119, CD71 and C-Kit antigen using specific flow cytometry gates for proerythroblasts, basophilic, polychromatic and orthochromatic erythroblasts, and peripheral blood reticulocytes were counted. Erythropoietin stimulated erythropoiesis, as evidenced by increased reticulocytes after 72 h by 197% and proerythroblasts by 50% (p < 0.05). Lipopolysaccharide alone decreased proerythroblasts by 53% and basophilic erythroblasts by 75% (p < 0.05). Orthochromatic erythroblasts doubled after lipopolysaccharide exposure (p < 0.05) without any increase in reticulocytes. Lipopolysaccharide completely suppressed erythropoietin's stimulatory effects and evoked a maturation block at the late stage of erythropoiesis. Lipopolysaccharide could cause anaemia in sepsis.
authors.affiliation:  Staff Anaesthesiologist.
authors.forename: P
authors.initials: P
authors.lastname: Brendt
authors.affiliation:  Veterinarian and Laboratory Leader.
authors.forename: I
authors.initials: I
authors.lastname: Rehfeld
authors.affiliation:  Medical Student.
authors.forename: A
authors.initials: A
authors.lastname: Kamphausen
authors.affiliation:  Specialist in Transfusion Medicine and Medical Director, DRK-Blutspendedienst West, ZBST, Ratingen, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Kreissig
authors.affiliation:  Professor of Anaesthesiology and Intensive Care Therapy, and Chairman, Klinik für Anästhesiologie und Intensivmedizin, Universität Duisburg-Essen and Universitätsklinikum Essen, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Peters
mesh_terms: D000818:Animals; D004920:Erythropoiesis / Q000187:drug effects*; D004921:Erythropoietin / Q000008:administration & dosage / Q000097:blood; D005434:Flow Cytometry; D008070:Lipopolysaccharides / Q000097:blood / Q000633:toxicity*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D008070:Lipopolysaccharides; D004921:Erythropoietin