title: [The role of Th17 cells in immune thrombocytopenia mice models].
abstract: OBJECTIVE
  To investigate the role of Th17 cells in immune thrombocytopenia (ITP) mice model.
  
  
  METHODS
  ITP was induced by daily intraperitoneal injection of anti-platelet membrane CD41 antibody (MWReg30) into BALB/c mice, the mRNA expressions of Th17 cell associated transcription factors and cytokines in peripheral blood and spleen mononuclear cells were measured by real-time PCR, and the proportion of Th17 cells by FCM analysis.
  
  
  RESULTS
  The percentage of Th17 cell was significantly elevated in ITP mice both in splenocyte and peripheral blood as compared with that in normal controls (P<0.01). ITP mice had elevated mRNA expressions of IL-17F, IL-17A and IL-6 in splenocyte (P<0.05), and of IL-21 in peripheral blood (P<0.05). There was a positive correlation between IL-17A and IL-17F (r = 0.934, P = 0.000), and between IL-17A/IL-17F and IL-6 (r = 0.598, P = 0.001; r = 0. 619, P = 0.000).
  
  
  CONCLUSIONS
  Th17 cell might play an important role in the pathogenesis of ITP, at least involving in the clearance of platelets.
authors.affiliation: Department of Hematology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China.
authors.forename: Ting-ting
authors.initials: TT
authors.lastname: Wang
authors.forename: Hui-yuan
authors.initials: HY
authors.lastname: Li
authors.forename: Zhao
authors.initials: Z
authors.lastname: Wang
authors.forename: Ze-ping
authors.initials: ZP
authors.lastname: Zhou
authors.forename: Li
authors.initials: L
authors.lastname: Fu
authors.forename: Na
authors.initials: N
authors.lastname: Wei
authors.forename: Da-yong
authors.initials: DY
authors.lastname: Huang
authors.forename: Ren-chi
authors.initials: RC
authors.lastname: Yang
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D058504:Th17 Cells / Q000276:immunology*; D013921:Thrombocytopenia / Q000276:immunology*