title: Formulation development of the biocontrol agent Bacillus subtilis strain CPA-8 by spray-drying.
abstract: OBJECTIVE
  To prepare commercially acceptable formulations of Bacillus subtilis CPA-8 by spray-drying with long storage life and retained efficacy to control peach and nectarine brown rot caused by Monilinia spp.
  
  
  RESULTS
  CPA-8 24-h- and 72-h-old cultures were spray dried using 10% skimmed milk, 10% skimmed milk plus 10% MgSO(4) , 10% MgSO(4) and 20% MgSO(4) as carriers/protectants. All carriers/protectants gave good percentages of powder recovery (28-38%) and moisture content (7-13%). CPA-8 survival varied considerably among spray-dried 24-h- and 72-h-old cultures. Seventy-two hours culture spray dried formulations showed the highest survival (28-32%) with final concentration products of 1·6-3·3 × 10(9) CFU g(-1) , while viability of 24-h-old formulations was lower than 1%. Spray-dried 72-h-old formulations were selected to subsequent evaluation. Rehydration of cells with water provided a good recovery of CPA-8 dried cells, similar to other complex rehydration media tested. Spray-dried formulations stored at 4 ± 1 and 20 ± 1°C showed good shelf life during 6 months, and viability was maintained or slightly decreased by 0·2-0·3-log. CPA-8 formulations after 4- and 6 months storage were effective in controlling brown rot caused by Monilinia spp. on nectarines and peaches resulting in a 90-100% reduction in disease incidence.
  
  
  CONCLUSIONS
  Stable and effective formulations of biocontrol agent B. subtilis CPA-8 could be obtained by spray-drying.
  
  
  CONCLUSIONS
  New shelf-stable and effective formulations of a biocontrol agent have been obtained by spray-drying to control brown rot on peach.
authors.affiliation: IRTA, XaRTA-Postharvest, Lleida, Catalonia, Spain.
authors.forename: V
authors.initials: V
authors.lastname: Yánez-Mendizábal
authors.forename: I
authors.initials: I
authors.lastname: Viñas
authors.forename: J
authors.initials: J
authors.lastname: Usall
authors.forename: R
authors.initials: R
authors.lastname: Torres
authors.forename: C
authors.initials: C
authors.lastname: Solsona
authors.forename: M
authors.initials: M
authors.lastname: Abadias
authors.forename: N
authors.initials: N
authors.lastname: Teixidó
mesh_terms: D001203:Ascomycota; D001412:Bacillus subtilis / Q000145:classification / Q000502:physiology; D003890:Desiccation; D050296:Microbial Viability; D010572:Pest Control, Biological; D010935:Plant Diseases / Q000382:microbiology / Q000517:prevention & control*; D027861:Prunus / Q000382:microbiology*