title: Identification of clinically relevant Corynebacterium spp., Arcanobacterium haemolyticum, and Rhodococcus equi by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
abstract: The identification of 83 Corynebacterium, 13 Arcanobacterium haemolyticum, and 10 Rhodococcus equi strains by conventional methods (API Coryne complemented with 16S rRNA gene sequence analysis) was compared with matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry identification. The correlation between API and MALDI-TOF results was 89%. MALDI-TOF is a rapid and accurate system for identification of the above-mentioned microorganisms.
authors.affiliation: Department of Clinical Microbiology, Hospital Clínic, CRESIB/IDIBAPS, School of Medicine, University of Barcelona, Barcelona, Spain. jvila@ub.edu
authors.forename: Jordi
authors.initials: J
authors.lastname: Vila
authors.forename: Pedro
authors.initials: P
authors.lastname: Juiz
authors.forename: Carlos
authors.initials: C
authors.lastname: Salas
authors.forename: Manel
authors.initials: M
authors.lastname: Almela
authors.forename: Celia García
authors.initials: CG
authors.lastname: de la Fuente
authors.forename: Yuliya
authors.initials: Y
authors.lastname: Zboromyrska
authors.forename: Jesús
authors.initials: J
authors.lastname: Navas
authors.forename: Jordi
authors.initials: J
authors.lastname: Bosch
authors.forename: Jesús
authors.initials: J
authors.lastname: Agüero
authors.forename: Jorge Puig
authors.initials: JP
authors.lastname: de la Bellacasa
authors.forename: Luis
authors.initials: L
authors.lastname: Martínez-Martínez
mesh_terms: D000818:Animals; D055667:Arcanobacterium / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification*; D001431:Bacteriological Techniques / Q000379:methods*; D003352:Corynebacterium / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification*; D006801:Humans; D016929:Rhodococcus equi / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013997:Time Factors