title: Functional study of Villin 2 protein expressed in longissimus dorsi muscle of Korean native cattle in different growth stages.
abstract: The aim of this study was to investigate protein profiles related to the induction of adipogenesis within the bovine longissimus dorsi muscle (BLDM) by proteomic analysis. We analyzed BLDM proteins at different growth stages to clarify the physiological mechanisms of marbled muscle development in 20 head of Korean native cattle (11 month: 10 head, 17 month: 10 head). BLDM proteins were analyzed by two-dimensional electrophoresis and image analysis. Villin 2 was specifically identified by mass spectrometry and a protein search engine. Villin 2 protein expression in BLDM decreased during the fat development stage in test steers. In a Western blot cell culture study of spontaneously immortal bovine muscle fibroblasts, the abundance of Villin 2 was shown to be down-regulated during differentiation into muscle. In 3T3-L1 mouse embryonic fibroblasts, Villin 2 was decreased during differentiation into adipocytes. The results suggest that Villin 2 may be related to the induction of transdifferentiation and adipogenesis in bovine longissimus dorsi muscle.
authors.affiliation: Department of Animal Science/Bio-resources and Development Institute Pusan National University, Miryang, Gyeongnam 627-706, Korea.
authors.forename: Yong-Cheng
authors.initials: YC
authors.lastname: Jin
authors.forename: Jeng-A
authors.initials: JA
authors.lastname: Han
authors.forename: Cheng-Xiong
authors.initials: CX
authors.lastname: Xu
authors.forename: Sang-Kee
authors.initials: SK
authors.lastname: Kang
authors.forename: Sang-Hun
authors.initials: SH
authors.lastname: Kim
authors.forename: Kang-Suk
authors.initials: KS
authors.lastname: Seo
authors.forename: Du-Hak
authors.initials: DH
authors.lastname: Yoon
authors.forename: Yun-Jaie
authors.initials: YJ
authors.lastname: Choi
authors.forename: Hong-Gu
authors.initials: HG
authors.lastname: Lee
mesh_terms: D041721:3T3-L1 Cells; D050156:Adipogenesis; D000818:Animals; D002417:Cattle; D002454:Cell Differentiation; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D008297:Male; D051379:Mice; D024510:Muscle Development; D018482:Muscle, Skeletal / Q000378:metabolism*; D056910:Republic of Korea; D000099149:Ezrin
chemical_list: D003598:Cytoskeletal Proteins; D000099149:Ezrin