title: Longitudinal and parallel monitoring of neuroinflammation and neurodegeneration in a 6-hydroxydopamine rat model of Parkinson's disease.
abstract: As neuroinflammatory processes are involved in the pathogenesis of Parkinson's disease (PD), we achieved the longitudinal evaluation of them in parallel with the modifications of dopaminergic function at several time-points after 6-hydroxydopamine (6-OHDA) lesion in the rat mimicking an early stage of PD. After unilateral intrastriatal 6-OHDA administration, we quantified the temporal evolution of the 18 kDa translocator protein (TSPO), TH-immunoreactivity and dopamine transporters in the striatum and substantia nigra pars compacta (SNc) from 3- to 56-days postlesion (dpl). Increased binding of TSPO ligands used, i.e., [(3)H]PK11195 and [(125)I]CLINDE, was observed in the lesioned striatum at 3, 7, and 14 dpl, followed by a progressive return to the basal level at 56 dpl. The binding profile in the SNc showed progressive binding beginning at 3 dpl, peaking at 14 dpl, and progressively decreasing until 56 dpl. In this model, the neuroinflammatory and neurodegenerative processes occurred concomitantly. The transitory occurrence of microglial activation could be involved in the lasting installation of dopaminergic neuron loss.
authors.affiliation: Inserm, U930, Tours, F-37000, France.
authors.forename: Serge
authors.initials: S
authors.lastname: Maia
authors.forename: Nicolas
authors.initials: N
authors.lastname: Arlicot
authors.forename: Emilie
authors.initials: E
authors.lastname: Vierron
authors.forename: Sylvie
authors.initials: S
authors.lastname: Bodard
authors.forename: Jackie
authors.initials: J
authors.lastname: Vergote
authors.forename: Denis
authors.initials: D
authors.lastname: Guilloteau
authors.forename: Sylvie
authors.initials: S
authors.lastname: Chalon
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D002352:Carrier Proteins / Q000378:metabolism; D003342:Corpus Striatum / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D004195:Disease Models, Animal; D059290:Dopaminergic Neurons / Q000378:metabolism*; D007249:Inflammation / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology; D008137:Longitudinal Studies; D017628:Microglia / Q000378:metabolism; D009410:Nerve Degeneration / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology; D010300:Parkinson Disease / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D051381:Rats; D017208:Rats, Wistar; D011963:Receptors, GABA-A / Q000378:metabolism; D013378:Substantia Nigra / Q000378:metabolism / Q000473:pathology; D013997:Time Factors
chemical_list: D002352:Carrier Proteins; D011963:Receptors, GABA-A; C069997:Tspo protein, rat