title: On-line removal of redox-active interferents by a porous electrode before amperometric blood glucose determination.
abstract: A porous reticulated vitreous carbon (RVC) electrode and a disk electrode coupled in tandem in an electrochemical flow cell has been used for electrolytic removal of interferents before amperometric glucose detection. The electrolytic efficiency at the upstream RVC electrode is 100% at a flow rate of 0.1 mL min(-1) or lower. Potential interferents such as acetaminophen, ascorbic acid, and uric acid can be completely eliminated by electrolysis at the RVC electrode. A mixed monolayer comprising glucose oxidase (GOD) and ferrocenyl-1-undecanethiol preformed at the downstream gold disk electrode was used as a mediator-based amperometric glucose sensor. The dependence of the amperometric current on the glucose concentration exhibits good linearity across over three orders of magnitude. The glucose measurements were also found to be reproducible (RSD<3.5%) and accurate. Unlike the chemiluminescence method, this device obviates the use of carcinogenic substrates and the glucose sensor performance is independent of the oxygen present in sample. On the basis that the RVC electrode requires minimal cleanup and the GOD-modified electrode remains stable for a week, the electrochemical flow cell should be amenable for automated on-line removal of redox interferents for other types of enzyme-based biosensors.
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan 410083, People's Republic of China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Deng
authors.forename: Yong
authors.initials: Y
authors.lastname: Peng
authors.forename: Lei
authors.initials: L
authors.lastname: Su
authors.forename: You-Nian
authors.initials: YN
authors.lastname: Liu
authors.forename: Feimeng
authors.initials: F
authors.lastname: Zhou
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D001786:Blood Glucose / Q000032:analysis* / Q000378:metabolism; D002244:Carbon / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000295:instrumentation* / Q000379:methods; D004566:Electrodes; D004867:Equipment Design; D005949:Glucose Oxidase / Q000378:metabolism; D006801:Humans; D010084:Oxidation-Reduction; D016062:Porosity
chemical_list: D001786:Blood Glucose; D002244:Carbon; D005949:Glucose Oxidase