title: Molecular cytogenetic identification of a novel dwarf wheat line with introgressed Thinopyrum ponticum  chromatin.
abstract: Novel dwarfing germplasms and dwarfing genes are valuable for the wheat breeding. A novel semi-dwarf line, 31505-1, with reduced height compared with its common wheat parent, was derived from a cross between common wheat and Thinopyrum ponticum. Cytological studies demonstrated that 31505-1 contained 42 chromosomes and formed 21 bivalents at meiotic metaphase I. Genomic in situ hybridization (GISH) analysis showed that 31505-1 had no large Th. ponticum chromosome fragments. Fluorescence in situ hybridization (FISH) results revealed the absence of a pAs1 hybridization band on 2DL chromosome of 31505-1. Two SSR markers (Xwmc41 and Xcfd168) and two STS markers (Xmag4059 and Xmag3596), which were located on 2D chromosome, amplified unique bands of Th. Ponticum in 31505-1. These revealed presence of an introgressed Th. ponticum segment in 2DL chromosome of dwarf line 31505-1, although the alien segment could not be detected by GISH.
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Tai'an Subcenter of National Wheat Improvement Center, Agronomy College, Shandong Agricultural University, Taian 271018, Shandong, China.
authors.forename: Guiling
authors.initials: G
authors.lastname: Chen
authors.forename: Qi
authors.initials: Q
authors.lastname: Zheng
authors.forename: Yinguang
authors.initials: Y
authors.lastname: Bao
authors.forename: Shubing
authors.initials: S
authors.lastname: Liu
authors.forename: Honggang
authors.initials: H
authors.lastname: Wang
authors.forename: Xingfeng
authors.initials: X
authors.lastname: Li
mesh_terms: D002843:Chromatin / Q000235:genetics*; D003433:Crosses, Genetic; D020732:Cytogenetic Analysis; D005819:Genetic Markers / Q000235:genetics; D005828:Genetics, Population; D017403:In Situ Hybridization; D017404:In Situ Hybridization, Fluorescence; D006109:Poaceae / Q000235:genetics*; D013045:Species Specificity; D014908:Triticum / Q000235:genetics* / Q000254:growth & development
chemical_list: D002843:Chromatin; D005819:Genetic Markers