title: Antibody-conjugated PEGylated cerium oxide nanoparticles for specific targeting of Aβ aggregates modulate neuronal survival pathways.
abstract: Oxidative stress has been found to be associated with the progression of neurodegenerative diseases such as Alzheimer's, Parkinson's, Lou Gehrig's, etc. In the recent years, cerium oxide nanoparticles (CNPs) have been studied as potent antioxidant agents able to exert neuroprotective effects. This work reports polyethylene glycol (PEG)-coated and antibody-conjugated CNPs for the selective delivering to Aβ aggregates, and the protective effect against oxidative stress/Aβ-mediated neurodegeneration. In this study PEG-coated and anti-Aβ antibody-conjugated antioxidant nanoparticles (Aβ-CNPs-PEG) were developed, and their effects on neuronal survival and brain-derived neurotrophic factor (BDNF) signaling pathway were examined. Aβ-CNPs-PEG specifically targets the Aβ aggregates, and concomitant rescue of neuronal survival better than Aβ-CNPs, by modulating the BDNF signaling pathway. This proof of concept work may allow in the future, once validated in vivo, for the selective delivery of CNPs only to affected brain areas.
authors.affiliation: Department of Basic and Applied Biology, University of L'Aquila, L'Aquila, Italy.
authors.forename: Annamaria
authors.initials: A
authors.lastname: Cimini
authors.forename: Barbara
authors.initials: B
authors.lastname: D'Angelo
authors.forename: Soumen
authors.initials: S
authors.lastname: Das
authors.forename: Roberta
authors.initials: R
authors.lastname: Gentile
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Benedetti
authors.forename: Virendra
authors.initials: V
authors.lastname: Singh
authors.forename: Antonina Maria
authors.initials: AM
authors.lastname: Monaco
authors.forename: Sandro
authors.initials: S
authors.lastname: Santucci
authors.forename: Sudipta
authors.initials: S
authors.lastname: Seal
mesh_terms: D016229:Amyloid beta-Peptides / Q000378:metabolism*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002470:Cell Survival; D002563:Cerium / Q000737:chemistry*; D005455:Fluorescent Antibody Technique; D006801:Humans; D047468:Immunoprecipitation; D053758:Nanoparticles; D009474:Neurons / Q000166:cytology*; D011092:Polyethylene Glycols / Q000737:chemistry*
chemical_list: D016229:Amyloid beta-Peptides; D002563:Cerium; D011092:Polyethylene Glycols; C030583:ceric oxide