title: A microfluidic device for bacteria detection in aqueous samples.
abstract: We demonstrate the use of a microfluidic device that accurately monitors bacteria levels in water. Fluorescent antibody labelled E. coli K12 bacteria in aqueous samples are flowed through the device, and spikes in the emission signal correspond to the presence of bacteria. Target bacteria could also be detected while suspended in a suspension of very low antibody concentration, suggesting that washing of bacteria could be avoided to make the detection automated and more rapid. Use of a microfluidic device will eventually enable quick on-site detection of bacteria using small quantities of sample.
authors.affiliation: Department of Chemical Engineering, University of Rhode Island, Kingston, RI 02881, USA.
authors.forename: Ashish K
authors.initials: AK
authors.lastname: Jha
authors.forename: Anubhav
authors.initials: A
authors.lastname: Tripathi
authors.forename: Arijit
authors.initials: A
authors.lastname: Bose
mesh_terms: D058491:Bacterial Load / Q000295:instrumentation*; D004784:Environmental Monitoring / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D048168:Escherichia coli K12 / Q000302:isolation & purification*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014871:Water Microbiology; D014873:Water Pollutants / Q000032:analysis*
chemical_list: D014873:Water Pollutants