title: Continuous, high capacity reconstitution of nutrient media from concentrated intermediates.
abstract: We designed an Integrated Media Preparation System (IMPS) for continuous, on-line preparation of cell culture media and delivery to intermediate storage vessels or directly to a bioreactor. Key components of the IMPS include: a high precision, continuous fluid mixing device; formulation-specific liquid medium concentrates; validated process controls and membrane filtration; and automated dispensing into large volume flexible plastic containers. The IMPS system is designed to produce sterile, single-strength liquid medium from common raw materials at a delivery rate of 1000-3000 liters per hour and will manufacture homogenous batches from several thousand liters to over 60,000 liters. Fortified nutrient media prepared from multi-component 50X concentrates have been demonstrated to accelerate bioreactor seed chains, increase product yield, and reduce the overall manufacturing cost of nutrient medium. A productivity matrix will analyze the fully-loaded costs and contrast alternative methods for media preparation against projected biological yield.
authors.affiliation: GIBCO-BRL, Life Technologies, Inc., 3175 Staley Road, 14072, Grand Island, NY, USA.
authors.forename: D W
authors.initials: DW
authors.lastname: Jayme
authors.forename: J M
authors.initials: JM
authors.lastname: Kubiak
authors.forename: T A
authors.initials: TA
authors.lastname: Battistoni
authors.forename: D J
authors.initials: DJ
authors.lastname: Cady