title: A mutation of OSOTP 51 leads to impairment of photosystem I complex assembly and serious photo-damage in rice.
abstract: Gene expression in chloroplasts is regulated by many nuclear-encoded proteins. In this study, we isolated a rice (Oryza sativa subsp. japonica) mutant osotp51 with significant reduction in photosystem I (PSI). The osotp51 is extremely sensitive to light and accumulates a higher level of reactive oxygen species. Its leaves are almost albino when grown at 40 μmol photons/m(2) per s. However, grown at 4 μmol photons/m(2) per s, osotp51 has a similar phenotype to the wild-type. 77K chlorophyll fluorescence analysis showed a blue shift in the highest peak emission from PSI in osotp51. In addition, the level of PSI and PSII dimer is dramatically reduced in osotp51. OSOTP 51 encodes a pentatricopeptide repeats protein, homologous to organelle transcript processing 51 in Arabidopsis. Loss-of-function OSOTP51 affects intron splicing of a number of plastid genes, particularly the ycf3 coding a protein involved in the assembly of PSI complex. OSOTP51 is functionally conserved in higher plants. The mutation of osotp51 indirectly leads to a widespread change in the structure and functions of PSI, results in severe photoinhibition, and finally dies, even when grown under very low light intensity.
authors.affiliation: National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
authors.forename: Jian-Wei
authors.initials: JW
authors.lastname: Ye
authors.forename: Zi-Ying
authors.initials: ZY
authors.lastname: Gong
authors.forename: Chun-Guang
authors.initials: CG
authors.lastname: Chen
authors.forename: Hua-Ling
authors.initials: HL
authors.lastname: Mi
authors.forename: Gen-Yun
authors.initials: GY
authors.lastname: Chen
mesh_terms: D015153:Blotting, Western; D002734:Chlorophyll / Q000378:metabolism; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects; D007438:Introns / Q000235:genetics; D008027:Light; D009154:Mutation / Q000235:genetics*; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D012275:Oryza / Q000235:genetics* / Q000254:growth & development / Q000528:radiation effects*; D010641:Phenotype; D045331:Photosystem I Protein Complex / Q000235:genetics*; D045332:Photosystem II Protein Complex / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism / Q000528:radiation effects / Q000648:ultrastructure; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D012326:RNA Splicing / Q000235:genetics / Q000528:radiation effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D036226:Seedlings / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects; D013050:Spectrometry, Fluorescence; D020524:Thylakoids / Q000378:metabolism / Q000528:radiation effects / Q000648:ultrastructure; D013997:Time Factors
chemical_list: D045331:Photosystem I Protein Complex; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D002734:Chlorophyll; D009580:Nitroblue Tetrazolium