title: Array-based capture, distribution, counting and multiplexed assaying of beads on a centrifugal microfluidic platform.
abstract: We present a novel centrifugal microfluidic platform for the highly efficient manipulation and analysis of particles for applications in bead-based assays. The platform uses an array of geometrical V-cup barriers to trap particles using stopped-flow sedimentation under highly reproducible hydrodynamic conditions. The impact parameters governing the occupancy distribution and capture efficiency of the arrayed traps are investigated. The unique, nearly 100% capture efficiency paired with the capability to establish sharply peaked, single occupancy distributions enables a novel, digital readout mode for color-multiplexed, particle-based assays with low-complexity instrumentation. The presented technology marks an essential step towards a versatile platform for the integration of bead- and cell-based biological assays.
authors.affiliation: Biomedical Diagnostics Institute, National Centre for Sensor Research, School of Physical Sciences, Dublin City University, Glasnevin, Dublin 9, Ireland. robert.burger2@mail.dcu.ie
authors.forename: Robert
authors.initials: R
authors.lastname: Burger
authors.forename: Patrick
authors.initials: P
authors.lastname: Reith
authors.forename: Gregor
authors.initials: G
authors.lastname: Kijanka
authors.forename: Victor
authors.initials: V
authors.lastname: Akujobi
authors.forename: Patrick
authors.initials: P
authors.lastname: Abgrall
authors.forename: Jens
authors.initials: J
authors.lastname: Ducrée
mesh_terms: D000906:Antibodies / Q000276:immunology; D002498:Centrifugation; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D006801:Humans; D007118:Immunoassay; D007074:Immunoglobulin G / Q000737:chemistry / Q000276:immunology; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D010316:Particle Size; D011137:Polystyrenes / Q000737:chemistry
chemical_list: D000906:Antibodies; D004129:Dimethylpolysiloxanes; D007074:Immunoglobulin G; D011137:Polystyrenes; C013830:baysilon