title: Noninvasive near-infrared live imaging of human adult mesenchymal stem cells transplanted in a rodent model of Parkinson's disease.
abstract: BACKGROUND
  We have previously shown that human mesenchymal stem cells (hMSCs) can reduce toxin-induced neurodegeneration in a well characterized rodent model of Parkinson's disease. However, the precise mechanisms, optimal cell concentration required for neuroprotection, and detailed cell tracking need to be defined. We exploited a near-infrared imaging platform to perform noninvasive tracing following transplantation of tagged hMSCs in live parkinsonian rats.
  
  
  METHODS
  hMSCs were labeled both with a membrane intercalating dye, emitting in the near- infrared 815 nm spectrum, and the nuclear counterstain, Hoechst 33258. Effects of near-infrared dye on cell metabolism and proliferation were extensively evaluated in vitro. Tagged hMSCs were then administered to parkinsonian rats bearing a 6-hydroxydopamine-induced lesion of the nigrostriatal pathway, via two alternative routes, ie, intrastriatal or intranasal, and the cells were tracked in vivo and ex vivo using near-infrared technology.
  
  
  RESULTS
  In vitro, NIR815 staining was stable in long-term hMSC cultures and did not interfere with cell metabolism or proliferation. A significant near-infrared signal was detectable in vivo, confined around the injection site for up to 14 days after intrastriatal transplantation. Conversely, following intranasal delivery, a strong near-infrared signal was immediately visible, but rapidly faded and was completely lost within 1 hour. After sacrifice, imaging data were confirmed by presence/absence of the Hoechst signal ex vivo in coronal brain sections. Semiquantitative analysis and precise localization of transplanted hMSCs were further performed ex vivo using near-infrared imaging.
  
  
  CONCLUSIONS
  Near-infrared technology allowed longitudinal detection of fluorescent-tagged cells in living animals giving immediate information on how different delivery routes affect cell distribution in the brain. Near-infrared imaging represents a valuable tool to evaluate multiple outcomes of transplanted cells, including their survival, localization, and migration over time within the host brain. This procedure considerably reduces the number of animal experiments needed, as well as interindividual variability, and may favor the development of efficient therapeutic strategies promptly applicable to patients.
authors.affiliation: Fondazione Matarelli, Dipartimento di Farmacologia, Chemioterapia e Tossicologia Medica, Università degli Studi di Milano, Milan, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Bossolasco
authors.forename: L
authors.initials: L
authors.lastname: Cova
authors.forename: G
authors.initials: G
authors.lastname: Levandis
authors.forename: V
authors.initials: V
authors.lastname: Diana
authors.forename: S
authors.initials: S
authors.lastname: Cerri
authors.forename: G
authors.initials: G
authors.lastname: Lambertenghi Deliliers
authors.forename: E
authors.initials: E
authors.lastname: Polli
authors.forename: V
authors.initials: V
authors.lastname: Silani
authors.forename: F
authors.initials: F
authors.lastname: Blandini
authors.forename: M T
authors.initials: MT
authors.lastname: Armentero
mesh_terms: D000281:Administration, Intranasal; D000704:Analysis of Variance; D000818:Animals; D006690:Bisbenzimidazole; D049109:Cell Proliferation / Q000187:drug effects; D058948:Cell Tracking / Q000379:methods*; D004195:Disease Models, Animal; D005456:Fluorescent Dyes / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000737:chemistry / Q000166:cytology*; D057054:Molecular Imaging; D010300:Parkinson Disease / Q000601:surgery*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019265:Spectroscopy, Near-Infrared / Q000379:methods*; D014793:Visual Cortex / Q000601:surgery
chemical_list: D005456:Fluorescent Dyes; D006690:Bisbenzimidazole