title: Development and validation of an HPLC-DAD method for quantification of bornesitol in extracts from Hancornia speciosa leaves after derivatization with p-toluenesulfonyl chloride.
abstract: Cylitol L-(+)-bornesitol is regarded as a bioactive constituent from Hancornia speciosa leaves, a plant species traditionally used in Brazil to treat diabetes and hypertension. We report a simple HPLC-DAD method for the quantification of bornesitol in extracts from H. speciosa leaves, after derivatization with p-toluenesulfonyl chloride, using pentaerythritol as internal standard. A gradient of methanol, acetonitrile and water was employed for elution on an ODS column and detection was set up at a wavelength of 230 nm. The method was selective and linear over the range 60.4-302.0 μg/ml with r² of 0.9981, and showed satisfactory precision for intra-day (RSD=2.37%) and inter-day (RSD=3.17%) assays. The recovery varied between 92.3% and 99.9% and the limits of quantification and detection were respectively 5.00 and 1.67 μg/ml. The method was applied to quantify bornesitol in extracts from H. speciosa leaves of different specimens.
authors.affiliation: Department of Pharmaceutical Products, Faculty of Pharmacy, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Ana Bárbara Dias
authors.initials: AB
authors.lastname: Pereira
authors.forename: Talisson Machado
authors.initials: TM
authors.lastname: Veríssimo
authors.forename: Mariana Assíria de
authors.initials: MA
authors.lastname: Oliveira
authors.forename: Ivaldo Antônio de
authors.initials: IA
authors.lastname: Araujo
authors.forename: Ricardo José
authors.initials: RJ
authors.lastname: Alves
authors.forename: Fernão Castro
authors.initials: FC
authors.lastname: Braga
mesh_terms: D029062:Apocynaceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D054812:Cyclitols / Q000032:analysis* / Q000737:chemistry; D004355:Drug Stability; D016014:Linear Models; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014105:Tosyl Compounds / Q000737:chemistry*
chemical_list: D054812:Cyclitols; D010936:Plant Extracts; D014105:Tosyl Compounds; C025506:4-toluenesulfonyl chloride