title: Development of a solid-phase extraction coupling chemiluminescent enzyme immunoassay for determination of organophosphorus pesticides in environmental water samples.
abstract: Solid-phase extraction (SPE) and direct competitive chemiluminescence enzyme immunoassay (dcCL-EIA) were combined for the detection of organophosphorus pesticides (OPs) in environmental water samples. dcCL-EIA based on horseradish peroxidase labeled with a broad-specificity monoclonal antibody against OPs was developed, and the effects of several physicochemical parameters on dcCL-EIA performance were studied. SPE was used for the pretreatment of water samples to remove interfering substances and to concentrate the OP analytes. The coupling of SPE and dcCL-EIA can detect seven OPs (parathion, coumaphos, phoxim, quinalphos, triazophos, dichlofenthion, and azinphos-ethyl) with the limit of quantitation below 0.1 ng/mL. The recoveries of OPs from spiked water samples ranged from 62.5% to 131.7% by SPE-dcCL-EIA and 69.5% to 112.3% by SPE-HPLC-MS/MS. The screening of OP residues in real-world environmental water samples by the developed SPE-dcCL-EIA and their confirmatory analysis using SPE-HPLC-MS/MS demonstrated that the assay is ideally suited as a monitoring method for OP residues prior to chromatographic analysis.
authors.affiliation: Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou, China.
authors.forename: Zhen-Lin
authors.initials: ZL
authors.lastname: Xu
authors.forename: Wen-Jia
authors.initials: WJ
authors.lastname: Sun
authors.forename: Jin-Yi
authors.initials: JY
authors.lastname: Yang
authors.forename: Yue-Ming
authors.initials: YM
authors.lastname: Jiang
authors.forename: Katrina
authors.initials: K
authors.lastname: Campbell
authors.forename: Yu-Dong
authors.initials: YD
authors.lastname: Shen
authors.forename: Hong-Tao
authors.initials: HT
authors.lastname: Lei
authors.forename: Dao-Ping
authors.initials: DP
authors.lastname: Zeng
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Yuan-Ming
authors.initials: YM
authors.lastname: Sun
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D002851:Chromatography, High Pressure Liquid; D005260:Female; D007124:Immunoenzyme Techniques / Q000379:methods*; D008163:Luminescent Measurements / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D009943:Organophosphorus Compounds / Q000032:analysis*; D010575:Pesticides / Q000032:analysis*; D052616:Solid Phase Extraction / Q000379:methods*; D053719:Tandem Mass Spectrometry; D014867:Water / Q000032:analysis; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal; D009943:Organophosphorus Compounds; D010575:Pesticides; D014874:Water Pollutants, Chemical; D014867:Water