title: Growth stimulation of tumor-specific cytotoxic T lymphocytes on concanavalin a-immobilized carrier beads.
abstract: Human tumor-specific CD4(+) cytotoxic T lymphocytes (CTL) were generated against duodenum papilloma cell line TGBC18TKB from HLA type-matched peripheral blood mononuclear cells. Concanavalin A (Con A) immobilized on carrier beads stimulated growth of the CTL in a long-term culture without repeated antigen stimulation, while soluble Con A induced death of the CTL. The CTL exhibited the target-specific cytotoxicity in a more potent manner than those before the long-term culture in the presence of the immobilized Con A. Enhanced expression of the adhesion molecule, CD11b, was observed on the CTL. These results suggest that immobilized Con A will be useful for continuous growth stimulation and large scale expansion of CTL without tumor antigen.
authors.affiliation: RIKEN Cell Bank, The Institute of Physical and Chemical Research (RIKEN), Koyadai 3-1-1, Tsukuba Science City, 305, Japan.
authors.forename: S Q
authors.initials: SQ
authors.lastname: Liu
authors.forename: L S
authors.initials: LS
authors.lastname: Liu
authors.forename: T
authors.initials: T
authors.lastname: Ohno