title: Effects of ethanol on hippocampal neurogenesis depend on the conditioned appetitive response.
abstract: Neurogenesis in the subgranular layer of the dentate gyrus (DG) has been suggested to underlie some forms of associative learning. The present study was undertaken to determine whether there was also a role of neurogenesis in the ethanol (EtOH)-induced conditioned place preference (CPP). Outbreed Swiss mice were conditioned with EtOH (2.0 g/kg) in one compartment of a non-biased place preference chamber and saline in the other compartment. This procedure produced three groups of mice: some developed a conditioned preference (EtOH_Cpp), others developed a conditioned avoidance (EtOH_Cpa) and still others demonstrated indifference to the context previously paired with ethanol (EtOH_Ind). BrdU (40 mg/kg, i.p.) was administered 4 hours after each session comprising the conditioning phase. When measured 24 hours following the CPP test, there was no effect of EtOH on doublecortin (DCX) expression or Fluoro Jade B staining. However, there were decreases in the number of BrdU+ and Ki-67+ cells in the EtOH_Cpa and EtOH_Ind groups, but not in the EtOH_Cpp group. Most of BrdU+ cells were co-labeled with DCX. Similarly, in another experiment, in that the perfusion was done 28 days after CPP test, most BrdU+ cells were co-localized with NeuN. These results suggest that conditioned appetitive response is able to maintain normal levels of neurogenesis in DG and might counteract ethanol-produced decreased cell proliferation/survival rate.
authors.affiliation: Laboratorio de Neurobiologia, Group of Neuronal Plasticity and Psychiatric Disorders, Universidade Federal de São Paulo, Brazil.
authors.forename: Carolina
authors.initials: C
authors.lastname: Tesone-Coelho
authors.forename: Patricia
authors.initials: P
authors.lastname: Varela
authors.forename: João C
authors.initials: JC
authors.lastname: Escosteguy-Neto
authors.forename: Clarissa F
authors.initials: CF
authors.lastname: Cavarsan
authors.forename: Luiz E
authors.initials: LE
authors.lastname: Mello
authors.forename: Jair G
authors.initials: JG
authors.lastname: Santos-Junior
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D021461:Animals, Outbred Strains; D001070:Appetitive Behavior / Q000502:physiology*; D001245:Association Learning / Q000187:drug effects / Q000502:physiology; D001362:Avoidance Learning / Q000187:drug effects; D016739:Behavior, Addictive / Q000523:psychology; D001973:Bromodeoxyuridine / Q000008:administration & dosage; D002452:Cell Count; D016923:Cell Death / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D003213:Conditioning, Psychological / Q000187:drug effects; D004268:DNA-Binding Proteins; D018891:Dentate Gyrus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D000431:Ethanol / Q000008:administration & dosage / Q000494:pharmacology*; D005452:Fluoresceins; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006799:Housing, Animal; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000032:analysis; D009419:Nerve Tissue Proteins / Q000378:metabolism; D055495:Neurogenesis / Q000187:drug effects*; D009479:Neuropeptides / Q000032:analysis; D009687:Nuclear Proteins / Q000378:metabolism; D010641:Phenotype; D012054:Reinforcement, Psychology; D013194:Staining and Labeling
chemical_list: D004268:DNA-Binding Proteins; C000714847:Dcx protein, mouse; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D005452:Fluoresceins; D019394:Ki-67 Antigen; D008869:Microtubule-Associated Proteins; C498355:Mki67 protein, mouse; D009419:Nerve Tissue Proteins; C492380:NeuN protein, mouse; D009479:Neuropeptides; D009687:Nuclear Proteins; C435731:fluoro jade; D000431:Ethanol; D001973:Bromodeoxyuridine