title: Capillary electrophoresis-inductively coupled plasma-mass spectrometry hyphenation for the determination at the nanogram scale of metal affinities and binding constants of phosphorylated ligands.
abstract: A screening strategy based on hyphenated capillary electrophoresis and inductively coupled plasma mass spectrometry (CE-ICP-MS) was developed to classify phosphorylated ligands according to their europium(III) binding affinity in a hydro-organic medium (sodium formate, pH 3.7, H(2)O/MeOH 90:10, v/v). Taking advantage of the high sensibility of ICP-MS for detecting phosphorus, this method enabled to assess the affinity of a variety of phosphorylated compounds, including phosphine oxides, thiophosphines, phosphonates, and phosphinates, in less than 1h and using less than 5 ng of substance. By varying the total europium concentration, complexation constants could be determined according to a sequential multiple run strategy, which proved to be in excellent agreement with the values obtained by UV-Vis absorption spectrophotometric titrations.
authors.affiliation: UMR CNRS 7178 IPHC-DSA, ECPM, Laboratoire d'Ingénierie Moléculaire Appliquée à l'Analyse, ECPM Bât R1N0, 25 rue Becquerel, 67087 Strasbourg, France.
authors.forename: Fanny
authors.initials: F
authors.lastname: Varenne
authors.forename: Mélanie
authors.initials: M
authors.lastname: Bourdillon
authors.forename: Michel
authors.initials: M
authors.lastname: Meyer
authors.forename: Yi
authors.initials: Y
authors.lastname: Lin
authors.forename: Marie
authors.initials: M
authors.lastname: Brellier
authors.forename: Rachid
authors.initials: R
authors.lastname: Baati
authors.forename: Loïc J
authors.initials: LJ
authors.lastname: Charbonnière
authors.forename: Alain
authors.initials: A
authors.lastname: Wagner
authors.forename: Eric
authors.initials: E
authors.lastname: Doris
authors.forename: Frédéric
authors.initials: F
authors.lastname: Taran
authors.forename: Agnès
authors.initials: A
authors.lastname: Hagège
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D005063:Europium / Q000737:chemistry*; D005561:Formates; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D008024:Ligands; D057230:Limit of Detection; D016014:Linear Models; D013058:Mass Spectrometry / Q000379:methods*; D000432:Methanol; D017553:Phosphorus Compounds / Q000737:chemistry*; D025341:Principal Component Analysis; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics
chemical_list: D005561:Formates; D028581:Lanthanoid Series Elements; D008024:Ligands; D017553:Phosphorus Compounds; C030544:formic acid; D005063:Europium; D000432:Methanol