title: [Effect of methylation modification on the expression of Cosmc gene in peripheral B lymphocyte of IgA nephropathy patients].
abstract: OBJECTIVE
  To investigate the effect of methylation modification on the mRNA expression of Cosmc gene in peripheral B lymphocytes from IgA nephropathy (IgAN).
  
  
  METHODS
  Biopsy identified 22 cases of IgAN patients and 20 cases of normal control were included. Peripheral B lymphocytes were isolated and were cultured with RPMI1640 medium, with LPS (12.5 microg/mL) as well as demethylation agent (5-AZA 0.1 micromol/L, 0.5 micromol/ L and 1.0 micromol/L) for 72 hours, respectively. Expression level of Cosmc gene was measured using real-time PCR.
  
  
  RESULTS
  The mRNA expression level of Cosmc gene in IgAN patients was significantly lower than that of control (P<0.05). The Cosmc gene expression level increased dramatically after RPMI1640 treatment (P<0.05), however, LPS could apparently reverse this effect (P<0.05). De-methylation modification up regulated the Cosmc gene expression significantly (P<0.05).
  
  
  CONCLUSIONS
  De-methylation modification could effectively reverse the repressed Comsc gene mRNA expression caused by external suppressors.
authors.affiliation: Department of Nephrology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Qin
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhong
authors.forename: Jun-ming
authors.initials: JM
authors.lastname: Fan
authors.forename: Xian-rong
authors.initials: XR
authors.lastname: Liu
authors.forename: Zi
authors.initials: Z
authors.lastname: Li
authors.forename: Xing-yi
authors.initials: XY
authors.lastname: Ma
mesh_terms: D000293:Adolescent; D000328:Adult; D001374:Azacitidine / Q000494:pharmacology; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D019175:DNA Methylation / Q000187:drug effects / Q000235:genetics*; D005260:Female; D005922:Glomerulonephritis, IGA / Q000235:genetics* / Q000276:immunology*; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D055815:Young Adult
chemical_list: C505369:C1GALT1C1 protein, human; D008070:Lipopolysaccharides; D018832:Molecular Chaperones; D012333:RNA, Messenger; D001374:Azacitidine