title: Colorimetric sensing of clenbuterol using gold nanoparticles in the presence of melamine.
abstract: A highly sensitive method for the detection of trace amount of clenbuterol based on gold nanoparticles (AuNPs) in the presence of melamine was described in this paper. Hydrogen-bonding interaction between clenbuterol and melamine resulted in the aggregation of AuNPs and a consequent color change of AuNPs from wine red to blue. The concentration of clenbuterol could be determined with naked eye or a UV-vis spectrometer. Results showed that the absorption ratio (A(670)/A(520)) was liner with the logarithm of clenbuterol concentration in the range of 2.8×10(-10) to 2.8×10(-7)M and 2.8×10(-7) to 1.4×10(-6)M with linear coefficients of 0.996 and 0.993, respectively. The detection limit was 2.8×10(-11)M (S/N=3), which was much lower than most existing methods. The coexisting substances including dl-epinephrine, phenylalamine, tryptohan, alamine, uric acid, glycine, glycerol, glucose, MgCl(2), CaCl(2) and NaCl did not affect the determination of clenbuterol. The proposed method could be successfully applied to the determination of clenbuterol in human urine.
authors.affiliation: College of Chemistry and Chemical Engineering, Graduate University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Zhang
authors.forename: Hong
authors.initials: H
authors.lastname: Zhao
authors.forename: Ying
authors.initials: Y
authors.lastname: Xue
authors.forename: Zhijiao
authors.initials: Z
authors.lastname: Wu
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yujian
authors.initials: Y
authors.lastname: He
authors.forename: Xiangjun
authors.initials: X
authors.lastname: Li
authors.forename: Zhuobin
authors.initials: Z
authors.lastname: Yuan
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002976:Clenbuterol / Q000302:isolation & purification* / Q000652:urine; D006046:Gold / Q000737:chemistry; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry; D012680:Sensitivity and Specificity; D014227:Triazines / Q000737:chemistry
chemical_list: D014227:Triazines; D006046:Gold; C011907:melamine; D002976:Clenbuterol