title: Polysaccharide and extracts from Lentinula edodes: structural features and antiviral activity.
abstract: BACKGROUND
  Lentinula edodes, known as shiitake, has been utilized as food, as well as, in popular medicine, moreover, compounds isolated from its mycelium and fruiting body have shown several therapeutic properties. The aim of this study was to determine the antiviral activity of aqueous (AqE) and ethanol (EtOHE) extracts and polysaccharide (LeP) from Lentinula edodes in the replication of poliovirus type 1 (PV-1) and bovine herpes virus type 1 (BoHV-1).
  
  
  METHODS
  The time-of-addition assay was performed at the times -2, -1, 0, 1 and 2 h of the infection. The virucidal activity and the inhibition of viral adsorption were also evaluated. Plaque assay was used to monitor antiviral activity throughout.
  
  
  RESULTS
  The AqE and LeP were more effective when added at 0 h of infection, however, EtOHE was more effective at the times 1 h and 2 h of the infection. AqE, EtOHE and LeP showed low virucidal activity, and the inhibition of viral adsorption was not significant.
  
  
  CONCLUSIONS
  The results allowed us to conclude that AqE, EtOHE and LeP act on the initial processes of the replication of both strains of virus.
authors.affiliation: Departamento de Microbiologia, Universidade Estadual de Londrina, Rod, Celso Garcia Cid,, km 380, CEP: 86051-990, Londrina, Paraná, Brazil.
authors.forename: Vinicius Pires
authors.initials: VP
authors.lastname: Rincão
authors.forename: Kristie Aimi
authors.initials: KA
authors.lastname: Yamamoto
authors.forename: Nágila Maria Pontes Silva
authors.initials: NM
authors.lastname: Ricardo
authors.forename: Sandra Aguiar
authors.initials: SA
authors.lastname: Soares
authors.forename: Luzia Doretto Paccola
authors.initials: LD
authors.lastname: Meirelles
authors.forename: Carlos
authors.initials: C
authors.lastname: Nozawa
authors.forename: Rosa Elisa Carvalho
authors.initials: RE
authors.lastname: Linhares
mesh_terms: D000998:Antiviral Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D007242:Herpesvirus 1, Bovine / Q000187:drug effects; D050296:Microbial Viability / Q000187:drug effects; D017955:Poliovirus / Q000187:drug effects; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D020942:Shiitake Mushrooms / Q000737:chemistry*; D053585:Virus Attachment / Q000187:drug effects; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D000998:Antiviral Agents; D011134:Polysaccharides