title: Biofunctionalized dendritic polyaniline nanofibers for sensitive electrochemical immunoassay of biomarkers.
abstract: Multi-armed dendritic polyaniline nanofibers (MPANFs) were first synthesized and functionalized with horseradish peroxidase (HRP) and carcinoembryonic antibody (anti-CEA) for highly efficient electrochemical immunoassay of carcinoembryonic antigen (CEA, as a model analyte here) in this work. Transmission electron microscope (TEM) and scanning electron microscope (SEM) techniques were employed to characterize the synthesized MPANFs. By using anti-CEA-conjugated core-shell gold-Fe(3)O(4) nanocomposites (GoldMag) as immunosensing probes and biofunctionalized MPANFs as molecular tags, a new sandwich-type homogeneous immunoassay strategy was developed for the determination of CEA by coupling with a home-made flow-through magneto-controlled microfluidic device. Under optimal conditions, the electrochemical immunoassay exhibited a wide dynamic range of four orders of magnitude from 1.0 pg mL(-1) to 50 ng mL(-1) with a low detection limit of 0.1 pg mL(-1) CEA at 3σ. Intra- and inter-assay coefficients of variation were below 10%. The assayed results for clinical serum specimens with the electrochemical immunoassay were received in good accordance with the results obtained from the referenced enzyme-linked immunosorbent assay (ELISA) method.
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, 350108 Fuzhou, P.R. China.
authors.forename: Yuling
authors.initials: Y
authors.lastname: Cui
authors.forename: Dianping
authors.initials: D
authors.lastname: Tang
authors.forename: Bingqian
authors.initials: B
authors.lastname: Liu
authors.forename: Huafeng
authors.initials: H
authors.lastname: Chen
authors.forename: Bing
authors.initials: B
authors.lastname: Zhang
authors.forename: Guonan
authors.initials: G
authors.lastname: Chen
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry*; D015415:Biomarkers / Q000737:chemistry*; D050091:Dendrimers / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004563:Electrochemistry; D005290:Ferric Compounds / Q000737:chemistry*; D007118:Immunoassay / Q000379:methods*; D046529:Microscopy, Electron, Transmission; D015394:Molecular Structure; D057139:Nanofibers / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D000814:Aniline Compounds; D015415:Biomarkers; D050091:Dendrimers; D005290:Ferric Compounds; C416807:polyaniline; C000499:ferric oxide