title: Coherent anti-stokes Raman scattering microscopy for high speed non- staining biomolecular imaging.
abstract: Vibrational microscopy (Raman microscopy and infrared microscopy), which observes molecular vibrations, gives us the information of molecular species without staining because the observed signals are originated from intrinsic molecules of a cell. However, infrared radiation is absorbed with water, and the long wavelength (3-10 μm) limits the spatial resolution to several micrometers. Spontaneous emission of Raman scattering is quite feeble, and the Raman scattering often overlaps with one-photon fluorescence from a specimen. Coherent anti-Stokes Raman scattering (CARS) microscopy, which is one of the nonlinear Raman microscopy, is a method to overcome those problems. In this review, present system of CARS microscopy, the methods of background rejection, and applications are introduced.
authors.affiliation: Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka, 560-8531, Japan. mamoru@me.es.osaka-u.ac.jp
authors.forename: Mamoru
authors.initials: M
authors.lastname: Hashimoto
authors.forename: Takeo
authors.initials: T
authors.lastname: Minamikawa
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Araki
mesh_terms: D016477:Artifacts; D008027:Light; D008853:Microscopy / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods; D061848:Optical Imaging / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*