title: [Effect of miR-15a on induction of apoptosis in breast cancer MCF-7 cells].
abstract: OBJECTIVE
  To investigate the effect and mechanism of miR-15a on the induction of apoptosis in breast cancer cells.
  
  
  METHODS
  To detect the expression level of miR-15a in breast cancer cell line MCF-7 cells and human mammary gland epithelial cell line MCF-10A cells by quantitative PCR. The target point of MCF-7 was predicted by software and was validated by luciferase report gene system. MiR-15a was transfected into MCF-7 cells with liposomes. The expression of Bcl-2 in MCF-7 cells was detected by Western blotting and the apoptosis rate of MCF-7 cells was detected by flow cytometry.
  
  
  RESULTS
  The expression level of miR-15a in MCF-7 cells was lower than that in the MCF-10A cells (0.253:1, P < 0.0001). The expression of MiR-15a was significantly inhibited by Bcl-2 (P < 0.05). Compared with the control, Bcl-2 expression was significantly decreased in the MCF-7 cells. The results of flow cytometry showed that the apoptosis rate was 13.4% in non-transfected MCF-7 cells, 15.9% in MCF-7 cells transfected with control RNA, and 31.5% in MCF-7 cells transfected with miR-15a (P < 0.05), indicating an evident induction of apoptosis in the MCF-7 cells.
  
  
  CONCLUSIONS
  miR-15a may have a potential application value in breast carcinoma biotherapy.
authors.affiliation: Hangzhou Normal University, Hangzhou, China. yuhua801229@163.com
authors.forename: Yu-hua
authors.initials: YH
authors.lastname: Liu
authors.forename: Li-quan
authors.initials: LQ
authors.lastname: Hong
authors.forename: Wang-qi
authors.initials: WQ
authors.lastname: Yu
authors.forename: Xiang-yun
authors.initials: XY
authors.lastname: Li
authors.forename: Xiao-yin
authors.initials: XY
authors.lastname: Zheng
mesh_terms: D017209:Apoptosis; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology; D006801:Humans; D061986:MCF-7 Cells; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D014162:Transfection
chemical_list: C505361:MIRN15 microRNA, human; D035683:MicroRNAs; D019253:Proto-Oncogene Proteins c-bcl-2