title: Autologous periodontal ligament cells in the treatment of Class III furcation defects: a study in dogs.
abstract: OBJECTIVE
  This study aimed to evaluate, histomorphometrically, the use of periodontal ligament cells (PDL cells) in the treatment of class III furcation defects.
  
  
  METHODS
  PDL cells were obtained from the mandibular tooth extracted from each dog (7), cultured in vitro and phenotypically characterized. Bilateral class III furcation defects were created at mandibular 3rd and 4th premolars and were randomly assigned to:
  
  
  METHODS
  coronally positioned flap, GTR Group: GTR, Sponge Group: carrier + GTR, Cell Group: carrier + PDL cells + GTR.
  
  
  RESULTS
  After 3 months of healing, data analysis demonstrated that the Cell Group presented a superior length of new cementum (4.82 ± 0.61 mm; 3.66 ± 0.95 mm; 2.87 ± 0.74 mm and 1.70 ± 0.60 mm, p < 0.001), a greater extension of periodontal regeneration (3.43 ± 1.44 mm; 2.33 ± 0.95 mm; 1.52 ± 0.39 mm and 0.69 ± 0.59 mm, p = 0.001) and a larger area of new bone (5.45 ± 1.58 mm(2) ; 3.94 ± 1.52 mm(2) ; 2.91 ± 0.56 mm(2)  and 1.89 ± 0.95 mm(2) , p = 0.0012), when compared with Sponge, GTR and CONTROL GROUP, respectively.
  
  
  CONCLUSIONS
  The PDL cells in association with GTR may significantly promote periodontal regeneration in class III furcation defects surgically created in dogs.
authors.affiliation: Department of Prosthodontics and Periodontics, Division of Periodontics, School of Dentistry at Piracicaba, State University of Campinas, São Paulo, Brazil.
authors.forename: Fabricia Ferreira
authors.initials: FF
authors.lastname: Suaid
authors.forename: Fernanda Vieira
authors.initials: FV
authors.lastname: Ribeiro
authors.forename: Thaisângela Rodrigues Lopes E Silva
authors.initials: TR
authors.lastname: Gomes
authors.forename: Karina Gonzales
authors.initials: KG
authors.lastname: Silvério
authors.forename: Marcelo Diniz
authors.initials: MD
authors.lastname: Carvalho
authors.forename: Francisco Humberto
authors.initials: FH
authors.lastname: Nociti
authors.forename: Marcio Zaffalon
authors.initials: MZ
authors.lastname: Casati
authors.forename: Enilson Antônio
authors.initials: EA
authors.lastname: Sallum
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D002478:Cells, Cultured; D003739:Dental Cementum / Q000502:physiology; D004285:Dogs; D017823:Furcation Defects / Q000601:surgery*; D016557:Guided Tissue Regeneration, Periodontal; D010513:Periodontal Ligament / Q000166:cytology*; D011897:Random Allocation; D012038:Regeneration; D033581:Stem Cell Transplantation; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds