title: The ISG15 conjugation system.
abstract: ISG15 is a ubiquitin-like modifier that is expressed in response to type 1 interferon signaling (IFN-α/β) and plays a role in antiviral responses. The core E1, E2, and E3 enzymes for ISG15 are Ube1L, UbcH8, and Herc5, respectively, and these are all also induced at the transcriptional level by IFN-α/β. We recently showed that Herc5 associates with polysomes and modifies target proteins in a cotranslational manner. Here, we describe the expression of the core conjugating enzymes in human cells, the detection of ISG15 conjugates, and the methods for fractionation of Herc5 with polysomes.
authors.affiliation: Section of Molecular Genetics and Molecular Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX, USA.
authors.forename: Larissa A
authors.initials: LA
authors.lastname: Durfee
authors.forename: Jon M
authors.initials: JM
authors.lastname: Huibregtse
mesh_terms: D016207:Cytokines / Q000096:biosynthesis / Q000378:metabolism*; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D007370:Interferon Type I / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D025801:Ubiquitin; D044764:Ubiquitin-Activating Enzymes; D044763:Ubiquitin-Conjugating Enzymes; D014452:Ubiquitins / Q000096:biosynthesis / Q000378:metabolism*
chemical_list: D016207:Cytokines; C402395:HERC5 protein, human; D007370:Interferon Type I; D047908:Intracellular Signaling Peptides and Proteins; D025801:Ubiquitin; D014452:Ubiquitins; C074034:ISG15 protein, human; C471622:UBE2L6 protein, human; D044763:Ubiquitin-Conjugating Enzymes; C534879:UBA7 protein, human; D044764:Ubiquitin-Activating Enzymes