title: High-content screening data management for drug discovery in a small- to medium-size laboratory: results of a collaborative pilot study focused on user expectations as indicators of effectiveness.
abstract: High-content screening (HCS) technology provides a powerful vantage point to approach biological problems; it allows analysis of cell parameters, including changes in cell or protein movement, shape, or texture. As part of a collaborative pilot research project to improve bioscience research data integration, we identified HCS data management as an area ripe for advancement. A primary goal was to develop an integrated data management and analysis system suitable for small- to medium-size HCS programs that would improve research productivity and increase work satisfaction. A system was developed that uses Labmatrix, a Web-based research data management platform, to integrate and query data derived from a Cellomics STORE database. Focusing on user expectations, several barriers to HCS productivity were identified and reduced or eliminated. The impact of the project on HCS research productivity was tested through a series of 18 lab-requested integrated data queries, 7 of which were fully enabled, 7 partially enabled, and 4 enabled through data export to standalone data analysis tools. The results are limited to one laboratory, but this pilot suggests that through an "implementation research" approach, a network of small- to medium-size laboratories involved in HCS projects could achieve greater productivity and satisfaction in drug discovery research.
authors.affiliation: Wilmer Eye Institute, Baltimore, MD, USA.
authors.forename: Cynthia A
authors.initials: CA
authors.lastname: Berlinicke
authors.forename: Christopher F
authors.initials: CF
authors.lastname: Ackermann
authors.forename: Steve H
authors.initials: SH
authors.lastname: Chen
authors.forename: Christoph
authors.initials: C
authors.lastname: Schulze
authors.forename: Yakov
authors.initials: Y
authors.lastname: Shafranovich
authors.forename: Sahiti
authors.initials: S
authors.lastname: Myneni
authors.forename: Vimla L
authors.initials: VL
authors.lastname: Patel
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Donald J
authors.initials: DJ
authors.lastname: Zack
authors.forename: Mikael
authors.initials: M
authors.lastname: Lindvall
authors.forename: G Steven
authors.initials: GS
authors.lastname: Bova
mesh_terms: D000818:Animals; D055808:Drug Discovery / Q000379:methods*; D001330:Electronic Data Processing / Q000379:methods*; D006801:Humans; D016490:Integrated Advanced Information Management Systems / Q000295:instrumentation* / Q000458:organization & administration*; D008403:Mass Screening / Q000379:methods*; D010865:Pilot Projects