title: [Analysis on the etiological and molecular epidemiological characteristics of Vibrio parahaemolyticus in Beijing].
abstract: OBJECTIVE
  To understand the etiological and molecular-epidemiological characteristics of Vibrio parahaemolyticus in Beijing.
  
  
  METHODS
  Stool specimens from sporadic diarrheal patients were collected during April to December, 2010. Culture and serotyping were used to detect the Vibrio parahaemolyticus from the 2118 specimens. All the positive strains were tested for drug sensitivity by Kirby-Bauer method. Real-time PCR was used to detect the existence of three virulence genes tlh, tdh and trh. Molecular typing on Vibrio parahaemolyticus isolates was completed by pulsed field gel electrophoresis (PFGE).
  
  
  RESULTS
  114 out of the 2118 specimens were Vibrio parahaemolyticus positive, with the positive rate as 5.38%. 114 isolates belonged to 23 serotypes, with the dominant (63.16%) serotype as O3:K6. Strains isolated from clinical manifestation patients were resistant to antibiotics-ampicillin and gentamicin, while with high sensitivity to amoxicillin, ceftriaxone, chloromycetin, imipenem, nalidixic acid and tetracycline. Virulence gene detection was positive to tlh for all the strains, but most to tdh, while only one strain to trh. The positive rate of tdh among O3:K6 strains (98.61%) was higher than that in those non-O3:K6 strains (85.71%) (P = 0.0098). 114 isolates were discriminated into 54 different PFGE patterns, while 72 O3:K6 strains into 34 patterns without the clustering characteristic.
  
  
  CONCLUSIONS
  Vibrio parahaemolyticus strains isolated from diarrheal patients in Beijing were dominated by O3:K6 strains, with stronger virulence. The positive rates of genes tlh and tdh were high. Vibrio parahaemolyticus strains showed high sensitivity to most antibiotics. The prevalent Vibrio parahaemolyticus strains in Beijing had various sources of clones.
authors.affiliation: Control of Endemic Infectious Diseases, Beijing Center for Disease Control and Prevention, Beijing, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Qu
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Gui-rong
authors.initials: GR
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Xi-tai
authors.initials: XT
authors.lastname: Li
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.forename: Han-qiu
authors.initials: HQ
authors.lastname: Yan
authors.forename: Bai-wei
authors.initials: BW
authors.lastname: Liu
authors.forename: Xin-yu
authors.initials: XY
authors.lastname: Li
authors.forename: Quan-yi
authors.initials: QY
authors.lastname: Wang
authors.forename: Fang
authors.initials: F
authors.lastname: Huang
mesh_terms: D002681:China / Q000453:epidemiology; D003967:Diarrhea / Q000453:epidemiology / Q000382:microbiology; D024881:Drug Resistance, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D005243:Feces / Q000382:microbiology; D006460:Hemolysin Proteins / Q000235:genetics*; D006801:Humans; D017720:Molecular Epidemiology; D012703:Serotyping; D014735:Vibrio Infections / Q000453:epidemiology* / Q000382:microbiology; D014736:Vibrio parahaemolyticus / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D006460:Hemolysin Proteins; D037521:Virulence Factors