title: Genome-wide identification, evolutionary expansion, and expression profile of homeodomain-leucine zipper gene family in poplar (Populus trichocarpa).
abstract: BACKGROUND
  Homeodomain-leucine zipper (HD-ZIP) proteins are plant-specific transcriptional factors known to play crucial roles in plant development. Although sequence phylogeny analysis of Populus HD-ZIPs was carried out in a previous study, no systematic analysis incorporating genome organization, gene structure, and expression compendium has been conducted in model tree species Populus thus far.
  
  
  RESULTS
  In this study, a comprehensive analysis of Populus HD-ZIP gene family was performed. Sixty-three full-length HD-ZIP genes were found in Populus genome. These Populus HD-ZIP genes were phylogenetically clustered into four distinct subfamilies (HD-ZIP I-IV) and predominately distributed across 17 linkage groups (LG). Fifty genes from 25 Populus paralogous pairs were located in the duplicated blocks of Populus genome and then preferentially retained during the sequential evolutionary courses. Genomic organization analyses indicated that purifying selection has played a pivotal role in the retention and maintenance of Populus HD-ZIP gene family. Microarray analysis has shown that 21 Populus paralogous pairs have been differentially expressed across different tissues and under various stresses, with five paralogous pairs showing nearly identical expression patterns, 13 paralogous pairs being partially redundant and three paralogous pairs diversifying significantly. Quantitative real-time RT-PCR (qRT-PCR) analysis performed on 16 selected Populus HD-ZIP genes in different tissues and under both drought and salinity stresses confirms their tissue-specific and stress-inducible expression patterns.
  
  
  CONCLUSIONS
  Genomic organizations indicated that segmental duplications contributed significantly to the expansion of Populus HD-ZIP gene family. Exon/intron organization and conserved motif composition of Populus HD-ZIPs are highly conservative in the same subfamily, suggesting the members in the same subfamilies may also have conservative functionalities. Microarray and qRT-PCR analyses showed that 89% (56 out of 63) of Populus HD-ZIPs were duplicate genes that might have been retained by substantial subfunctionalization. Taken together, these observations may lay the foundation for future functional analysis of Populus HD-ZIP genes to unravel their biological roles.
authors.affiliation: CAS Key Laboratory of Biofuels, Qingdao Institute of BioEnergy and BioProcess Technology, Chinese Academy of Sciences, Qingdao, Shandong, People's Republic of China.
authors.forename: Ruibo
authors.initials: R
authors.lastname: Hu
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Chi
authors.forename: Guohua
authors.initials: G
authors.lastname: Chai
authors.forename: Yingzhen
authors.initials: Y
authors.lastname: Kong
authors.forename: Guo
authors.initials: G
authors.lastname: He
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Wang
authors.forename: Dachuan
authors.initials: D
authors.lastname: Shi
authors.forename: Dongyuan
authors.initials: D
authors.lastname: Zhang
authors.forename: Gongke
authors.initials: G
authors.lastname: Zhou
mesh_terms: D019143:Evolution, Molecular; D020869:Gene Expression Profiling; D005801:Genes, Homeobox; D017343:Genes, Plant / Q000502:physiology; D018745:Genome, Plant / Q000235:genetics*; D016350:Leucine Zippers / Q000235:genetics*; D010802:Phylogeny; D032107:Populus / Q000235:genetics*; D013312:Stress, Physiological / Q000235:genetics; D014018:Tissue Distribution