title: [Analysis of gag-specific IFN-gamma secretion responses in HIV-1 B/C recombinant infectors].
abstract: OBJECTIVE
  To characterize the Gag-Specific T lymphocyte responses and identify immunodominant region recognized in Chinese HIV-1 recombinant subtype B/C infectors.
  
  
  METHODS
  10 antiretroviral treatment (ART) naive HIV-1 recombinant subtype B/C infectors with infected time in 1 year, 25 ART-naive infectors with infected time > 3 years and 10 HIV-1-seronegative healthy individuals were enrolled. HIV-1-specific T lymphocyte responses were analyzed by an IFN-gamma Elispot assay against 123 overlapping peptides spanning HIV-1 Gag protein in the present study.
  
  
  RESULTS
  Gag-specific T lymphocyte responses of interferon-gamma secretion were identified in 8(8/10) Chinese HIV-1 recombinant subtype B/ C infectors with infected time in 1 year, the specific T lymphocytes are mainly targeted at five seperated peptides. Responses were identified in 17(68%) infectors with infected time more than 3 years, the specific T lymphocytes are mainly targeted at one peptide in p17 and six in p24. There was obviously positive correlation (P = 0.0318, r = 0.519) between the magnitude of responses and viremia in infectors infected time > 3 years. The magnitude of response in infectors infected in 1 year was significantly higher than group infected time > 3 years (P = 0.021). None of healthy individuals produced positive responses.
  
  
  CONCLUSIONS
  HIV-1 recombinant subtype B/C Infectors at different stages of diseases recognize different region of gag.
authors.affiliation: Center for AIDS/STD Control and Prevention, Henan Center for Disease Control and Prevention, Zhengzhou 450016, China.
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Liu
authors.forename: Kun-Xue
authors.initials: KX
authors.lastname: Hong
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Liu
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Chen
authors.forename: Yu-Hua
authors.initials: YH
authors.lastname: Ruan
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wang
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Shao
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000821:virology; D015683:Gene Products, gag / Q000235:genetics / Q000276:immunology*; D015658:HIV Infections / Q000235:genetics / Q000276:immunology* / Q000821:virology; D015497:HIV-1 / Q000145:classification / Q000235:genetics* / Q000276:immunology*; D006801:Humans; D016056:Immunodominant Epitopes; D007371:Interferon-gamma / Q000235:genetics / Q000276:immunology*; D011995:Recombination, Genetic
chemical_list: D015683:Gene Products, gag; D016056:Immunodominant Epitopes; D007371:Interferon-gamma