title: [Establishment of multiple regression model for virulence factors of Saccharomyces albicans by random amplified polymorphic DNA bands].
abstract: OBJECTIVE
  To research the relationship between the virulence factors of Saccharomyces albicans (S. albicans) and the random amplified polymorphic DNA (RAPD) bands of them, and establish the regression model by multiple regression analysis.
  
  
  METHODS
  Extracellular phospholipase, secreted proteinase, ability to generate germ tubes and adhere to oral mucosal cells of 92 strains of S. albicans were measured in vitro; RAPD-polymerase chain reaction (RAPD-PCR) was used to get their bands. Multiple regression for virulence factors of S. albicans and RAPD-PCR bands was established.
  
  
  RESULTS
  The extracellular phospholipase activity was associated with 4 RAPD bands: 350, 450, 650 and 1 300 bp (P < 0.05); secreted proteinase activity of S. albicans was associated with 2 bands: 350 and 1 200 bp (P < 0.05); the ability of germ tube produce was associated with 2 bands: 400 and 550 bp (P < 0.05).
  
  
  CONCLUSIONS
  Some RAPD bands will reflect the virulence factors of S. albicans indirectly. These bands would contain some important messages for regulation of S. albicans virulence factors.
authors.affiliation: Dept. of Medical Microbiology and Immunology, School of Basic Medicine, Dali College, Dali 671000, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Liu
authors.forename: Youcong
authors.initials: Y
authors.lastname: Wu
authors.forename: Youhua
authors.initials: Y
authors.lastname: Yuan
authors.forename: Li
authors.initials: L
authors.lastname: Bai
authors.forename: Kun
authors.initials: K
authors.lastname: Niu
mesh_terms: D004247:DNA; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D012440:Saccharomyces; D037521:Virulence Factors
chemical_list: D037521:Virulence Factors; D004247:DNA