title: [Netrin-1 protein expression in gastric cancer and its correlation with clinicopathologic features and prognosis].
abstract: OBJECTIVE
  To evaluate the relationship between netrin-1 protein, clinicopathologic features and prognosis in gastric cancer patients.
  
  
  METHODS
  Tissue micro-array and immunohistochemistry were used to detect expression of netrin-1 protein and Ki67. And clinicopathological relevance of netrin-1 protein and Ki67 in gastric cancer were analyzed. Survival rates was evaluated by Kaplan-Meier survival curves. Cox regression analysis was performed to evaluate the possibility of netrin-1 expression as an independent prognostic factor for gastric cancer.
  
  
  RESULTS
  The positive-expression rate of netrin-1 protein, paracancerous netrin-1 protein and Ki67 in tumor tissue from 67 patients with gastric cancer were 49%, 42% and 60%, respectively. netrin-1 protein expression might be related to depth of invasion, lymth node metastasis and distant metastasis (P<0.05). But no correlation was observed in sex, tumor diameter and tumor grade (P>0.05). There was correlation between different degrees of netrin-1 expression and distant metastases (P<0.05). No correlation was found in the expression of Ki67 and clinicopathological features. The correlation between the expression of netrin-1 and Ki67 was observed (r=0.359, P<0.05). Using Kaplan-Meier survival curves and the log-rank test, the correlation of netrin-1 expression, different degrees of netrin-1 expression and survival (P<0.05) were also observed. But netrin-1 expression was not significantly correlated with the prognosis of gastric cancer (RR = 1.335, 95% CI: 0.612-2.914).
  
  
  CONCLUSIONS
  netrin-1 protein may be related with tumorigenesis and tumor progression by affecting proliferation. The detection for netrin-1 may be helpful to evaluate the clinicopathological parameters and survival time. However, it is not an independent prognostic factor for gastric cancer.
authors.affiliation: Department of Surgery, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China.
authors.forename: Jin-ming
authors.initials: JM
authors.lastname: Chen
authors.forename: Shen-hua
authors.initials: SH
authors.lastname: Wang
authors.forename: Shan-xiang
authors.initials: SX
authors.lastname: Xu
authors.forename: Bao-san
authors.initials: BS
authors.lastname: Han
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D046228:Microarray Analysis; D008875:Middle Aged; D009414:Nerve Growth Factors / Q000378:metabolism*; D000075388:Netrin-1; D011379:Prognosis; D016016:Proportional Hazards Models; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology; D025521:Tumor Suppressor Proteins / Q000378:metabolism*; D055815:Young Adult
chemical_list: D019394:Ki-67 Antigen; C000617351:NTN1 protein, human; D009414:Nerve Growth Factors; D025521:Tumor Suppressor Proteins; D000075388:Netrin-1