title: Visualization of microvascular proliferation as a tumor infiltration structure in rat glioma specimens using the diffraction-enhanced imaging in-plane CT technique.
abstract: In order to study potent microenvironments of malignant gliomas with a high- resolution x-ray imaging technique, an injection orthotopic glioma model was made using the Sprague-Dawley rat. Total brain tissue, taken out as an ex vivo model, was examined with diffraction-enhanced imaging (DEI) computed tomography (CT) acquired with a 35 keV monochromatic x-ray. In the convolution-reconstructed 2D/3D images with a spatial resolution of 12.5 × 12.5 × 25 µm, distinction among necrosis, typical ring-shaped viable tumors, edemas and healthy tissues was clearly observed near the frontal lobe in front of the rat's caudate nucleus. Multiple microvascular proliferations (MVPs) were observed surrounding peritumoral edemas as a tumor infiltration structure. Typical dimensions of tubular MVPs were 130 (diameter) ×250 (length) µm with a partial sprout structure revealed in the 3D reconstructed image. Hyperplasia of cells around vessel walls was revealed with tumor cell infiltration along the perivascular space in microscopic observations of mild MVP during histological analysis. In conclusion, DEI-CT is capable of imaging potent tumor-infiltrating MVP structures surrounding high-grade gliomas.
authors.affiliation: Biomedical Engineering and Radiology, School of Medicine, Catholic University of Daegu, Daegu 705-034, Korea.
authors.forename: Seung-Jun
authors.initials: SJ
authors.lastname: Seo
authors.forename: Naoki
authors.initials: N
authors.lastname: Sunaguchi
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Yuasa
authors.forename: Qingkai
authors.initials: Q
authors.lastname: Huo
authors.forename: Masami
authors.initials: M
authors.lastname: Ando
authors.forename: Gi-Hwan
authors.initials: GH
authors.lastname: Choi
authors.forename: Hong-Tae
authors.initials: HT
authors.lastname: Kim
authors.forename: Ki-Hong
authors.initials: KH
authors.lastname: Kim
authors.forename: Eun-Ju
authors.initials: EJ
authors.lastname: Jeong
authors.forename: Won-Seok
authors.initials: WS
authors.lastname: Chang
authors.forename: Jong-Ki
authors.initials: JK
authors.lastname: Kim
mesh_terms: D000179:Acrylates; D000818:Animals; D001932:Brain Neoplasms / Q000473:pathology* / Q000532:radiotherapy; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004487:Edema / Q000473:pathology; D004867:Equipment Design; D005910:Glioma / Q000473:pathology* / Q000532:radiotherapy; D007091:Image Processing, Computer-Assisted; D008833:Microcirculation; D015233:Models, Statistical; D009368:Neoplasm Transplantation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014057:Tomography, X-Ray Computed / Q000379:methods
chemical_list: D000179:Acrylates; C036658:acrylic acid