title: Biodegradation of buprofezin by Rhodococcus sp. strain YL-1 isolated from rice field soil.
abstract: A buprofezin-degrading bacterium, YL-1, was isolated from rice field soil. YL-1 was identified as Rhodococcus sp. on the basis of the comparative analysis of 16S rDNA sequences. The strain could use buprofezin as the sole source of carbon and nitrogen for growth and was able to degrade 92.4% of 50 mg L(-1) buprofezin within 48 h in liquid culture. During the degradation of buprofezin, four possible metabolites, 2-tert-butylimino-3-isopropyl-1,3,5-thiadiazinan-4-one, N-tert-butyl-thioformimidic acid formylaminomethyl ester, 2-isothiocyanato-2-methyl-propane, and 2-isothiocyanato-propane, were identified using gas chromatography-mass spectrometry (GC-MS) analysis. The catechol 2,3-dioxygenase activity was strongly induced during the degradation of buprofezin. A novel microbial biodegradation pathway for buprofezin was proposed on the basis of these metabolites. The inoculation of soils treated with buprofezin with strain YL-1 resulted in a higher degradation rate than that observed in noninoculated soils, indicating that strain YL-1 has the potential to be used in the bioremediation of buprofezin-contaminated environments.
authors.affiliation: Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing, Jiangsu Province, People's Republic of China.
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.forename: Ji
authors.initials: J
authors.lastname: Zhang
authors.forename: Zhi-Guo
authors.initials: ZG
authors.lastname: Wu
authors.forename: Li
authors.initials: L
authors.lastname: Cao
authors.forename: Xin
authors.initials: X
authors.lastname: Yan
authors.forename: Shun-Peng
authors.initials: SP
authors.lastname: Li
mesh_terms: D001673:Biodegradation, Environmental; D007605:Juvenile Hormones / Q000378:metabolism*; D012275:Oryza / Q000254:growth & development / Q000382:microbiology*; D012240:Rhodococcus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*; D013829:Thiadiazines / Q000378:metabolism*
chemical_list: D007605:Juvenile Hormones; D012989:Soil Pollutants; D013829:Thiadiazines; C083997:buprofezin