title: Contribution of IncFII and broad-host IncA/C and IncN plasmids to the local expansion and diversification of phylogroup B2 Escherichia coli ST131 clones carrying blaCTX-M-15 and qnrS1 genes.
abstract: The recent increase of CTX-M-15-producing Escherichia coli isolates in our institution was caused by diverse clonal backgrounds, including mainly B2 sequence type 131 (ST131) clones presenting variable virulence profiles but also A(1) (ST617, ST410), B1, and D(1) (ST405) clones. Besides IncFII-pC15-1a, we detected multidrug-resistant IncA/C(2) and IncN plasmids carrying bla(CTX-M-15) and/or qnrS1. Our study highlights the diversification of highly transmissible resistant and virulent clones and the recombinogenic potential of broad-host plasmids contributing to the expansion of genetic regions coding for multidrug resistance to other bacterial lineages.
authors.affiliation: Servicio de Microbiología and CIBER en Epidemiología y Salud Pública, Instituto Ramón y Cajal de Investigación Sanitaria and Hospital Universitario Ramón y Cajal, Madrid, Spain.
authors.forename: Ângela
authors.initials: Â
authors.lastname: Novais
authors.forename: Diana
authors.initials: D
authors.lastname: Viana
authors.forename: Fernando
authors.initials: F
authors.lastname: Baquero
authors.forename: Javier
authors.initials: J
authors.lastname: Martínez-Botas
authors.forename: Rafael
authors.initials: R
authors.lastname: Cantón
authors.forename: Teresa M
authors.initials: TM
authors.lastname: Coque
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002999:Clone Cells; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000235:genetics*; D022761:Gene Transfer, Horizontal; D058507:Host Specificity; D006801:Humans; D010802:Phylogeny; D010957:Plasmids / Q000235:genetics*; D011995:Recombination, Genetic; D013030:Spain; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D029968:Escherichia coli Proteins; C473109:beta-lactamase CTX-M-15; D001618:beta-Lactamases