title: Fall in oxygen tension of culture medium stimulates the adenosinergic signalling of a human T cell line.
abstract: We examined the short-course expression of various parameters involved in the adenosinergic signalling of a human T cell line during in vitro decrease of the medium culture oxygen tension mimicking in vivo hypoxia. Fall of 92 mmHg in oxygen tension of culture medium induced in CEM, a CD4+ human T cell line, a continuous production of hypoxia-inducing factor-1α with a plateau value at 9 h, a rapid increase in adenosine production peaking at 3 h and a decrease in adenosine deaminase peaking at 6 h. The adenosine A(2A) receptor (A(2A)R) protein level of CEM cells was enhanced with a peak at 6 h. Intracellular 3',5'-cyclic adenosine monophosphate accumulated in CEM cells with a maximal level at 9 h. These results show that a human-cultured T cells line can upregulate its own adenosine production and A(2A)R expression during exposure to acute hypoxia. Hypoxia-increased stimulation of the adenosinergic signalling of T cells may have immunosuppressive properties and, consequently, A(2A)R agonists may have therapeutic relevance.
authors.affiliation: UMR MD2 P2COE, Aix-Marseille Univ, Faculté de Médecine, Timone, 27, Boulevard Jean Moulin, F-13385, Marseille Cedex 5, France.
authors.forename: Youlet
authors.initials: Y
authors.lastname: By
authors.forename: Laurent
authors.initials: L
authors.lastname: Jacquin
authors.forename: Frédéric
authors.initials: F
authors.lastname: Franceschi
authors.forename: Josée-Martine
authors.initials: JM
authors.lastname: Durand-Gorde
authors.forename: Jocelyne
authors.initials: J
authors.lastname: Condo
authors.forename: Pierre
authors.initials: P
authors.lastname: Michelet
authors.forename: Régis
authors.initials: R
authors.lastname: Guieu
authors.forename: Jean
authors.initials: J
authors.lastname: Ruf
mesh_terms: D000241:Adenosine / Q000378:metabolism*; D015687:Cell Hypoxia; D002460:Cell Line; D003470:Culture Media; D000242:Cyclic AMP / Q000378:metabolism; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism*; D043705:Receptor, Adenosine A2A / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D013997:Time Factors; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D003470:Culture Media; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D043705:Receptor, Adenosine A2A; D000242:Cyclic AMP; D000241:Adenosine; D010100:Oxygen