title: Serum-free growth medium for the cultivation of a wide spectrum of mammalian cells in stirred bioreactors.
abstract: A serum free medium was developed, that could be used for the large scale propagation of various cell lines in bioreactors. The medium is based on a 1:1 mixture of Iscove's Modified Dulbecco's Medium and Ham's Medium F12, supplemented with transferrin, insulin and a BSA/oleic acid complex. Several myelomas, hybridomas derived from different myelomas and spleen cells, and other lymphoid and non-lymphoid cell lines were cultivated at growth rates comparable to those observed using serum-supplemented media. There was furthermore no reduction in the formation of products such as monoclonal antibodies or recombinant human interleukin-2.
authors.affiliation: Gesellschaft für Biotechnologische Forschung mbH, Mascheroder Weg 1, D-3300, Braunschweig, FRG.
authors.forename: V
authors.initials: V
authors.lastname: Jäger
authors.forename: J
authors.initials: J
authors.lastname: Lehmann
authors.forename: P
authors.initials: P
authors.lastname: Friedl