title: Moesin is a biomarker for the assessment of genotoxic carcinogens in mouse lymphoma.
abstract: 1,2-Dibromoethane and glycidol are well known genotoxic carcinogens, which have been widely used in industry. To identify a specific biomarker for these carcinogens in cells, the cellular proteome of L5178Y mouse lymphoma cells treated with these compounds was analyzed by 2-dimensional gel electrophoresis (2-DE) and MALDI-TOF mass spectrometry (MS). Of 50 protein spots showing a greater than 1.5-fold increase or decrease in intensity compared to control cells on a 2-D gel, we focused on the candidate biomarker moesin. Western analysis using monoclonal rabbit anti-moesin confirmed the identity of the protein and its increased level of expression upon exposure to the carcinogenic compounds. Moesin expression also increased in cells treated with six additional genotoxic carcinogens, verifying that moesin could serve as a biomarker to monitor phenotypic change upon exposure to genotoxic carcinogens in L5178Y mouse lymphoma cells.
authors.affiliation: Department of Biotechnology, Yonsei University, Seoul, 120-749, Korea.
authors.forename: Yoen Jung
authors.initials: YJ
authors.lastname: Lee
authors.forename: In-Kwon
authors.initials: IK
authors.lastname: Choi
authors.forename: Yhun Yhong
authors.initials: YY
authors.lastname: Sheen
authors.forename: Sue Nie
authors.initials: SN
authors.lastname: Park
authors.forename: Ho Jeong
authors.initials: HJ
authors.lastname: Kwon
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D002273:Carcinogens / Q000633:toxicity*; D045744:Cell Line, Tumor; D004249:DNA Damage; D004852:Epoxy Compounds / Q000633:toxicity*; D015946:Ethylene Dibromide / Q000633:toxicity*; D051379:Mice; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism*; D020005:Propanols / Q000633:toxicity*; D020543:Proteome; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; D002273:Carcinogens; D004852:Epoxy Compounds; D008840:Microfilament Proteins; D020005:Propanols; D020543:Proteome; C071058:moesin; D015946:Ethylene Dibromide; C004312:glycidol