title: Non-host resistance to penetration and hyphal growth of Magnaporthe oryzae in Arabidopsis.
abstract: Rice blast caused by Magnaporthe oryzae is a devastating disease of rice. Mechanisms of rice resistance to blast have been studied extensively, and the rice-M. oryzae pathosystem has become a model for plant-microbe interaction studies. However, the mechanisms of non-host resistance (NHR) to rice blast in other plants remain poorly understood. We found that penetration resistance to M. oryzae in multiple mutants, including pen2 NahG pmr5 agb1 and pen2 NahG pmr5 mlo2 plants, was severely compromised and that fungal growth was permitted in penetrated epidermal cells. Furthermore, rice Pi21 enhanced movement of infection hyphae from penetrated Arabidopsis epidermal cells to adjacent mesophyll cells. These results indicate that PEN2, PMR5, AGB1, and MLO2 function in both penetration and post-penetration resistance to M. oryzae in Arabidopsis, and suggest that the absence of rice Pi21 contributed to Arabidopsis NHR to M. oryzae.
authors.affiliation: Department of Bioscience, Fukui Prefectural University , Fukui, 910-1195,  Japan.
authors.forename: Misato
authors.initials: M
authors.lastname: Nakao
authors.forename: Ryotaro
authors.initials: R
authors.lastname: Nakamura
authors.forename: Kaori
authors.initials: K
authors.lastname: Kita
authors.forename: Ryuya
authors.initials: R
authors.lastname: Inukai
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ishikawa
mesh_terms: D000483:Alleles; D017360:Arabidopsis / Q000382:microbiology* / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000502:physiology; D060467:Disease Resistance; D044387:GTP-Binding Protein beta Subunits / Q000502:physiology; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D020082:Magnaporthe / Q000378:metabolism*; D008565:Membrane Proteins / Q000502:physiology; D009154:Mutation; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology*; D030821:Plants, Genetically Modified / Q000382:microbiology / Q000502:physiology
chemical_list: C089590:AGB1 protein, Arabidopsis; D029681:Arabidopsis Proteins; D044387:GTP-Binding Protein beta Subunits; C573497:MLO2 protein, Arabidopsis; D008565:Membrane Proteins; C499437:PMR5 protein, Arabidopsis