title: Interprotomer motion-transmission mechanism for the hexameric AAA ATPase p97.
abstract: Multimeric AAA ATPases represent a structurally homologous yet functionally diverse family of proteins. The essential and highly abundant hexameric AAA ATPase p97 is perhaps the best studied AAA protein, playing an essential role in various important cellular activities. During ATP-hydrolysis process, p97 undergoes dramatic conformational changes, and these changes are initiated in the C-terminal ATPase domain and transmitted across the entire length of the molecule to the N-terminal effector domain. However, the detailed mechanism of the motion transmission remains unclear. Here, we report an interprotomer motion-transmission mechanism to explain this process: The nucleotide-dependent motion transmission between the two ATPase domains of one protomer is mediated by its neighboring protomer. This finding reveals a strict requirement for interprotomer coordination of p97 during the motion-transmission process and may shed light on studies of other AAA ATPases.
authors.affiliation: Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY 11794-5215, USA.
authors.forename: Guangtao
authors.initials: G
authors.lastname: Li
authors.forename: Chengdong
authors.initials: C
authors.lastname: Huang
authors.forename: Gang
authors.initials: G
authors.lastname: Zhao
authors.forename: William J
authors.initials: WJ
authors.lastname: Lennarz
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry* / Q000502:physiology; D000255:Adenosine Triphosphate / Q000737:chemistry; D000818:Animals; D002469:Cell Separation; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005434:Flow Cytometry; D057809:HEK293 Cells; D006801:Humans; D006868:Hydrolysis; D051379:Mice; D008958:Models, Molecular; D008968:Molecular Conformation; D009038:Motion; D009154:Mutation; D009687:Nuclear Proteins / Q000737:chemistry* / Q000502:physiology; D009711:Nucleotides / Q000737:chemistry; D011487:Protein Conformation; D017434:Protein Structure, Tertiary
chemical_list: D009687:Nuclear Proteins; D009711:Nucleotides; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; C404942:p97 ATPase