title: Generation of mouse ES cell lines engineered for the forced induction of transcription factors.
abstract: Here we report the generation and characterization of 84 mouse ES cell lines with doxycycline-controllable transcription factors (TFs) which, together with the previous 53 lines, cover 7-10% of all TFs encoded in the mouse genome. Global gene expression profiles of all 137 lines after the induction of TFs for 48 hrs can associate each TF with the direction of ES cell differentiation, regulatory pathways, and mouse phenotypes. These cell lines and microarray data provide building blocks for a variety of future biomedical research applications as a community resource.
authors.affiliation: National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA.
authors.forename: Lina S
authors.initials: LS
authors.lastname: Correa-Cerro
authors.forename: Yulan
authors.initials: Y
authors.lastname: Piao
authors.forename: Alexei A
authors.initials: AA
authors.lastname: Sharov
authors.forename: Akira
authors.initials: A
authors.lastname: Nishiyama
authors.forename: Jean S
authors.initials: JS
authors.lastname: Cadet
authors.forename: Hong
authors.initials: H
authors.lastname: Yu
authors.forename: Lioudmila V
authors.initials: LV
authors.lastname: Sharova
authors.forename: Li
authors.initials: L
authors.lastname: Xin
authors.forename: Hien G
authors.initials: HG
authors.lastname: Hoang
authors.forename: Marshall
authors.initials: M
authors.lastname: Thomas
authors.forename: Yong
authors.initials: Y
authors.lastname: Qian
authors.forename: Dawood B
authors.initials: DB
authors.lastname: Dudekula
authors.forename: Emily
authors.initials: E
authors.lastname: Meyers
authors.forename: Bernard Y
authors.initials: BY
authors.lastname: Binder
authors.forename: Gregory
authors.initials: G
authors.lastname: Mowrer
authors.forename: Uwem
authors.initials: U
authors.lastname: Bassey
authors.forename: Dan L
authors.initials: DL
authors.lastname: Longo
authors.forename: David
authors.initials: D
authors.lastname: Schlessinger
authors.forename: Minoru S H
authors.initials: MS
authors.lastname: Ko
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D060846:Cell Engineering / Q000379:methods; D002460:Cell Line; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism*; D020869:Gene Expression Profiling; D005818:Genetic Engineering / Q000379:methods; D051379:Mice; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics*
chemical_list: D011994:Recombinant Proteins; D014157:Transcription Factors