title: Nucleophosmin expression in renal cell carcinoma and oncocytoma.
abstract: The objective of this study was to investigate nucleophosmin/B23 (NPM) expression in renal cell carcinomas (RCC) and renal oncocytomas. The expression of NPM was studied by immunohistochemical methods on 59 RCCs, 9 oncocytomas, and 19 tumour-negative renal tissues. The expression was assessed relative to various clinicopathological variables and histological subtypes, to determine its potential role as a prognostic and diagnostic marker. All tumours showed nuclear staining, and a minority also exhibited cytoplasmic immunoreactivity. Two patterns of nuclear staining were observed: nuclear staining with a prominent nucleolus (nucleolar staining) and nuclear staining without a prominent nucleolus. There were significant differences, in both nuclear staining and cytoplasmic NPM expression, between oncocytomas and chromophobe RCCs (p < 0.001) and clear cell RCCs (p < 0.001). Cytoplasmic NPM expression was markedly lower in RCCs than in oncocytomas. A statistically significant correlation was discovered between nucleolar staining and nuclear grade (p < 0.001, r = 0.5). No relationship was found between cytoplasmic expression of NPM and any of the clinicopathological parameters or survival. NPM might be a useful immunohistochemical marker for differential diagnosis between oncocytoma and chromophobe RCC. In addition, increased nucleolar NPM expression in RCCs appears to be associated with tumour progression.
authors.affiliation: Department of Pathology, Izmir Ataturk Training and Research Hospital, Turkey. aysegulakder@gmail.com
authors.forename: Aysegul
authors.initials: A
authors.lastname: Sari
authors.forename: Aylin
authors.initials: A
authors.lastname: Calli
authors.forename: Aysegul Aksoy
authors.initials: AA
authors.lastname: Altinboga
authors.forename: Fatma Seher
authors.initials: FS
authors.lastname: Pehlivan
authors.forename: Sacit Nuri
authors.initials: SN
authors.lastname: Gorgel
authors.forename: Ugur
authors.initials: U
authors.lastname: Balci
authors.forename: Murat
authors.initials: M
authors.lastname: Ermete
authors.forename: Cetin
authors.initials: C
authors.lastname: Dincel
authors.forename: Fulya
authors.initials: F
authors.lastname: Cakalagaoglu
mesh_terms: D018249:Adenoma, Oxyphilic / Q000378:metabolism* / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000096:biosynthesis*; D002292:Carcinoma, Renal Cell / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D007680:Kidney Neoplasms / Q000378:metabolism* / Q000473:pathology; D008875:Middle Aged; D060787:Neoplasm Grading; D009687:Nuclear Proteins / Q000096:biosynthesis*; D000090243:Nucleophosmin; D016612:Paraffin Embedding; D012189:Retrospective Studies; D018709:Statistics, Nonparametric; D016019:Survival Analysis
chemical_list: D014408:Biomarkers, Tumor; C000716967:NPM1 protein, human; D009687:Nuclear Proteins; D000090243:Nucleophosmin