title: Detection of influenza virus using a lateral flow immunoassay for amplified DNA by a microfluidic RT-PCR chip.
abstract: Influenza virus RNA was amplified by a continuous-flow polydimethylsiloxane microfluidic RT-PCR chip within 15-20 min. The amplified influenza virus RNA was observed with the naked eye, as the red color at the test line, using a lateral flow immunoassay within 1 min.
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Okayama University of Science, Okayama-shi 700-0005, Japan. nagatani@dac.ous.ac.jp
authors.forename: Naoki
authors.initials: N
authors.lastname: Nagatani
authors.forename: Keiichiro
authors.initials: K
authors.lastname: Yamanaka
authors.forename: Hiromi
authors.initials: H
authors.lastname: Ushijima
authors.forename: Ritsuko
authors.initials: R
authors.lastname: Koketsu
authors.forename: Tadahiro
authors.initials: T
authors.lastname: Sasaki
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Ikuta
authors.forename: Masato
authors.initials: M
authors.lastname: Saito
authors.forename: Toshiro
authors.initials: T
authors.lastname: Miyahara
authors.forename: Eiichi
authors.initials: E
authors.lastname: Tamiya
mesh_terms: D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D007118:Immunoassay / Q000379:methods*; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D009975:Orthomyxoviridae / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
chemical_list: D004279:DNA, Viral