title: Succinic acid fermentation in a stationary-basket bioreactor with a packed bed of immobilized Actinobacillus succinogenes: 1. Influence of internal diffusion on substrate mass transfer and consumption rate.
abstract: This paper is dedicated to the study on external and internal mass transfers of glucose for succinic fermentation under substrate and product inhibitions using a bioreactor with a stationary basket bed of immobilized Actinobacillus succinogenes cells. By means of the substrate mass balance for a single particle of biocatalysts, considering the Jerusalimsky kinetic model including both inhibitory effects, specific mathematical expressions have been developed for describing the profiles of the substrate concentrations and mass flows in the outer and inner regions of biocatalyst particles, as well as for estimating the influence of internal diffusion on glucose consumption rate. The results indicated that very low values of internal mass flow could be reached in the particles center. The corresponding region was considered biologically inactive, with its extent varying from 0.24% to 44% from the overall volume of each biocatalyst. By immobilization of bacterial cells and use of a basket bed, the rate of glucose consumption is reduced up to 200 times compared with the succinic fermentation system containing free cells.
authors.affiliation: Department of Biotechnologies, "Gr.T. Popa" University of Medicine and Pharmacy of Iasi, M. Kogalniceanu 9-13, 700454 Iasi, Romania.
authors.forename: Anca-Irina
authors.initials: AI
authors.lastname: Galaction
authors.forename: Lenuta
authors.initials: L
authors.lastname: Kloetzer
authors.forename: Marius
authors.initials: M
authors.lastname: Turnea
authors.forename: Colin
authors.initials: C
authors.lastname: Webb
authors.forename: Anestis
authors.initials: A
authors.lastname: Vlysidis
authors.forename: Dan
authors.initials: D
authors.lastname: Caşcaval
mesh_terms: D000188:Actinobacillus / Q000378:metabolism*; D000464:Alginates; D019149:Bioreactors / Q000382:microbiology*; D018914:Cells, Immobilized / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D007700:Kinetics; D019802:Succinic Acid / Q000378:metabolism*
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D019802:Succinic Acid; D005947:Glucose