title: Chitosan/collagen scaffold containing bone morphogenetic protein-7 DNA supports dental pulp stem cell differentiation in vitro and in vivo.
abstract: In this study, porous chitosan/collagen scaffolds were prepared through a freeze-drying process, and loaded with the plasmid vector encoding human bone morphogenetic protein-7 (BMP-7) gene. To investigate the feasibility and efficacy of this gene-activated scaffold on dental tissue engineering, human dental pulp stem cells (DPSCs) were seeded in this scaffold for in vitro and in vivo study. In vitro results indicated that cells can be transfected successfully by loaded plasmid and secrete BMP-7 until day 24. Evaluation of DNA content, ALP activity, calcium content, SEM, and real-time PCR revealed that cells on gene-activated scaffold showed better proliferation properties and odontoblastic differentiation behaviors than cells on pure scaffolds. Then, these cell-scaffold complexes were implanted subcutaneously and retrieved after 4 weeks for histology evaluation. In vivo results that gene-activated scaffold group could still trace the existence of tranfected cells at week 4 and showed the upregulated expression of DSPP compared to pure scaffold groups. On the basis of our results, chitosan/collagen-loaded BMP-7 DNA appears to be an effective substrate candidate for gene delivery and indeed enhanced DPSCs differentiation toward an odontoblast-like phenotype in vitro and in vivo. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2012.
authors.affiliation: Key Lab for Oral Biomedicine of Ministry of Education and Department of Endodontics, School and Hospital of Stomatology, Wuhan University, Wuhan, Hubei Province, People's Republic of China.
authors.forename: Xuechao
authors.initials: X
authors.lastname: Yang
authors.affiliation: Key Lab for Oral Biomedicine of Ministry of Education and Department of Endodontics, School and Hospital of Stomatology, Wuhan University, Wuhan, Hubei Province, People's Republic of China.
authors.forename: Guangli
authors.initials: G
authors.lastname: Han
authors.affiliation: Key Lab for Oral Biomedicine of Ministry of Education and Department of Endodontics, School and Hospital of Stomatology, Wuhan University, Wuhan, Hubei Province, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Pang
authors.affiliation: Key Lab for Oral Biomedicine of Ministry of Education and Department of Endodontics, School and Hospital of Stomatology, Wuhan University, Wuhan, Hubei Province, People's Republic of China.
authors.forename: Mingwen
authors.initials: M
authors.lastname: Fan
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D055419:Bone Morphogenetic Protein 7 / Q000737:chemistry / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects*; D003094:Collagen / Q000737:chemistry*; D004247:DNA / Q000737:chemistry / Q000494:pharmacology; D003782:Dental Pulp / Q000378:metabolism*; D005260:Female; D064593:Heterografts; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009804:Odontoblasts / Q000378:metabolism*; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000378:metabolism*
chemical_list: C525819:BMP7 protein, human; D055419:Bone Morphogenetic Protein 7; D003094:Collagen; D004247:DNA