title: Comparison of two different deposition methods of 3-aminopropyltriethoxysilane on glass slides and their application in the ThinPrep cytologic test.
abstract: In this work, two different deposition methods of 3-aminopropyltriethoxysilane (APTES) on glass slides were compared in order to study the adhesion effect of cervical exfoliated cells on smear slides. Glass slides were modified by vapor-phase deposition (V-D) and liquid-phase deposition (L-D), respectively. The topographic images and amine density of the modified slides were investigated by using atomic force microscopy, UV-vis spectroscopy and X-ray photoelectron spectroscopy. The numbers of cells adhered on the slides functionalized by V-D and L-D were counted and compared under the microscope. The data showed significant differences between the two methods (t-test: P < 0.05). The results presented here have made it theoretically possible to produce amine slides by V-D method for the ThinPrep cytologic test.
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, China.
authors.forename: Sen
authors.initials: S
authors.lastname: Xiang
authors.forename: Gaowa
authors.initials: G
authors.lastname: Xing
authors.forename: Wei
authors.initials: W
authors.lastname: Xue
authors.forename: Chao
authors.initials: C
authors.lastname: Lu
authors.forename: Jin-Ming
authors.initials: JM
authors.lastname: Lin
mesh_terms: D019411:Clinical Laboratory Techniques / Q000379:methods*; D005260:Female; D005898:Glass / Q000737:chemistry*; D006801:Humans; D018625:Microscopy, Atomic Force; D015394:Molecular Structure; D056951:Photoelectron Spectroscopy; D011437:Propylamines; D012821:Silanes / Q000737:chemistry*; D013499:Surface Properties; D014626:Vaginal Smears
chemical_list: D011437:Propylamines; D012821:Silanes; C477625:amino-propyl-triethoxysilane