title: Production and accumulation of 4-O-methyl-α-D-glucuronosyl-xylotriose by growing culture of thermophilic Anoxybacillus sp. strain JT-12.
abstract: A thermophilic Anoxybacillus sp. strain JT-12, isolated from soil, produced acidic xylotriose, 4-O-methyl-α-D-glucuronosyl-xylotriose (MeGlcAX₃), as a main product from birchwood xylan and accumulated them in the culture under optimum conditions at pH 7.0 and 55 °C using 0.75% (w/v) birchwood xylan as a carbon source for 42-72 h. The acidic xylotriose was purified by ethanol precipitation and high-performance liquid chromatography using NH₂ Lichosher® 100 column. The results of electrospray ionization mass spectrometry, mass to charge ratio (m/z) 603.23, confirmed that the purified sample was acidic xylotriose that had benefits and applications in many fields.
authors.affiliation: Division of Biochemical Technology, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkuntien, Bangkok 10150, Thailand.
authors.forename: Jitladda
authors.initials: J
authors.lastname: Thitikorn-amorn
authors.forename: Khin Lay
authors.initials: KL
authors.lastname: Kyu
authors.forename: Kazuo
authors.initials: K
authors.lastname: Sakka
authors.forename: Khanok
authors.initials: K
authors.lastname: Ratanakhanokchai
mesh_terms: D058677:Anoxybacillus / Q000378:metabolism*; D029662:Betula / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D000431:Ethanol / Q000737:chemistry; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D021241:Spectrometry, Mass, Electrospray Ionization; D014312:Trisaccharides / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism; D014934:Wood / Q000378:metabolism*; D014990:Xylans / Q000378:metabolism*
chemical_list: D003470:Culture Media; D014312:Trisaccharides; D014990:Xylans; D000431:Ethanol