title: Pleistocene climatic oscillations and the speciation history of an alpine endemic and a widespread arctic-alpine plant.
abstract: Even in cases in which geographic isolation appears to have driven the speciation of regional endemics, range shifts during the Pleistocene climatic oscillations may also have influenced their evolutionary history. Elucidating speciation history can provide novel insights into evolutionary dynamics following climatic oscillations. We demonstrated a sister relationship between the Japanese alpine endemic Cardamine nipponica and the currently allopatric, widespread arctic-alpine Cardamine bellidifolia (Brassicaceae) based on internal transcribed spacer (ITS) sequences and 10 other nuclear genes. Speciation history was inferred using demographic parameters under the isolation with migration model. The estimated demographic parameters showed that the population size of C. nipponica was similar to that of C. bellidifolia and that gene flow occurred exclusively from C. nipponica to C. bellidifolia after speciation. The inferred speciation history, which included gene flow, suggests that geographic barriers between the peripheral C. nipponica and the widespread C. bellidifolia were reduced during the Pleistocene. The asymmetric introgression implies that genetic isolation may have been involved in the speciation of C. nipponica. Our results suggest that even currently allopatric species may not have diverged solely under geographic isolation, and that their evolutionary history may have been influenced by Pleistocene range dynamics.
authors.affiliation: Department of Botany, National Museum of Nature and Science, 4-1-1 Amakubo, Tsukuba 305-0005, Ibaraki, Japan.
authors.forename: Hajime
authors.initials: H
authors.lastname: Ikeda
authors.affiliation: Microbial Evolution Research Group, Department of Biology, University of Oslo, PO Box 1066 Blindern, NO-0316 Oslo, Norway.
authors.forename: Tor
authors.initials: T
authors.lastname: Carlsen
authors.affiliation: Graduate School of Science and Technology, Kumamoto University, 2-39-1 Kurokami, Kumamoto, Kumamoto 860-8555, Japan.
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Fujii
authors.affiliation: National Centre for Biosystematics, Natural History Museum, University of Oslo, PO Box 1172 Blindern, NO-0318 Oslo, Norway.
authors.forename: Christian
authors.initials: C
authors.lastname: Brochmann
authors.affiliation: Graduate School of Human and Environmental Studies, Kyoto University, Yoshida-nihonmatsu-cho, Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Setoguchi
mesh_terms: D001110:Arctic Regions; D031217:Cardamine / Q000235:genetics*; D002467:Cell Nucleus / Q000235:genetics; D002980:Climate; D021901:DNA, Intergenic / Q000235:genetics; D017753:Ecosystem; D049810:Genetic Speciation; D005843:Geography; D007564:Japan; D016013:Likelihood Functions; D008954:Models, Biological; D008969:Molecular Sequence Data; D010802:Phylogeny; D011110:Polymorphism, Genetic; D011156:Population Density; D013045:Species Specificity; D013997:Time Factors
chemical_list: D021901:DNA, Intergenic