title: Biohydrogen production from co-digestion of cow manure and waste milk under thermophilic temperature.
abstract: Biohydrogen production from co-digestion of cow manure (M) and waste milk (WM), milk from mastitis cows treated with cefazolin, was evaluated in a 3×5 factorial design. Organic loading of 20, 40 and 60g volatile solid (VS)L(-1) were tested at temperature of 55°C using M:WM (VS/VS) 70:30, 50:50, 30:70, 10:90 and 0:100. Hydrogen production increased with organic loading and M:WM to a maximum of 59.5mLg(-1) VS fed at 40g VSL(-1) in M:WM 70:30. Butyrate was the main volatile fatty acid (VFA) accumulated in M:WM 50:50, 30:70 and 10:90. Overall reduction of more than 90% of cefazolin resistant bacteria was observed in all the treatments. The reduction was higher at 40 and 60 than 20g VSL(-1) (P<0.05). Inclusion of waste milk enhances hydrogen production from cow manure and could offer added benefit of waste milk treatment and disposal.
authors.affiliation: Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
authors.forename: Suraju A
authors.initials: SA
authors.lastname: Lateef
authors.forename: Nilmini
authors.initials: N
authors.lastname: Beneragama
authors.forename: Takaki
authors.initials: T
authors.lastname: Yamashiro
authors.forename: Masahiro
authors.initials: M
authors.lastname: Iwasaki
authors.forename: Chun
authors.initials: C
authors.lastname: Ying
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Umetsu
mesh_terms: D000818:Animals; D002417:Cattle; D006859:Hydrogen / Q000378:metabolism*; D008372:Manure; D008892:Milk / Q000378:metabolism*
chemical_list: D008372:Manure; D006859:Hydrogen