title: 5-Fluorouracil accumulation in green microalgae and its biogenetic transfer into ciliate protozoan.
abstract: The study has demonstrated that anticancer drug 5-fluorouracil causes acute toxicity and interferes with the growth of green microalgae, Scenedesmus vacuolatus. It accumulates in microalgae biomass with bioaccumulation factor of 1.84 × 10(4) and further integrates into the DNA and RNA of microalgae. In addition, the labelled microalgae genome is transferred into protozoan Tetrahymena pyriformis on feeding and is retained in the food vacuoles of predator organisms. This biotransfer of labelled 5-fluorouracil via genomic material was evaluated using radioactivity in Tetrahymena cell pellets though radioactivity did not detect anticancer drug in the genome of the predator organism.
authors.affiliation: Helmholtz Zentrum München-German Research Center for Environmental Health, Institute of Ecological Chemistry, Ingolstädter Landstrasse 1, 85764, Neuherberg, Germany. asad.uddaula@googlemail.com
authors.forename: Asad
authors.initials: A
authors.lastname: Ud-Daula
authors.forename: Gerd
authors.initials: G
authors.lastname: Pfister
authors.forename: Karl-Werner
authors.initials: KW
authors.lastname: Schramm
mesh_terms: D000460:Chlorophyta / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D005472:Fluorouracil / Q000378:metabolism* / Q000633:toxicity; D020387:Food Chain; D013769:Tetrahymena pyriformis / Q000235:genetics / Q000502:physiology*; D014162:Transfection
chemical_list: D005472:Fluorouracil