title: Observation of dimethylaminoethyl methacrylate-myoglobin binding reaction using laser spray mass spectrometry.
abstract: Covalent bonds are often created by a reaction between chemicals and protein before causing various adverse effects in a cell. Dimethylaminoethyl methacrylate (DMAEMA), which has moderate toxicity, causes skin inflammation and throat irritation. For this study, we investigated a reaction mechanism between myoglobin and (DMAEMA) using a new analytical tool developed at our laboratory: laser spray mass spectrometry technique. It was found that initially DMAEMA was added to the amino group of protein by the Michael addition mechanism; the added DMAEMA was hydrolyzed to methacrylic acid using an autocatalytic system. The results of this study indicate the feasibility of the laser spray technique in analyses of reaction dynamics.
authors.affiliation: PRESTO, Japan Science and Technology Agency, 4-1-8 Honcho Kawaguchi, Saitama 332-0012, Japan. atsushit@tmu.ac.jp
authors.forename: Atsushi
authors.initials: A
authors.lastname: Takamizawa
authors.forename: Kayoko
authors.initials: K
authors.lastname: Mishina
authors.forename: Kenzo
authors.initials: K
authors.lastname: Hiraoka
mesh_terms: D000818:Animals; D006736:Horses; D013058:Mass Spectrometry / Q000379:methods*; D008689:Methacrylates / Q000737:chemistry* / Q000378:metabolism; D009211:Myoglobin / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D013696:Temperature
chemical_list: D008689:Methacrylates; D009211:Myoglobin; C049840:2-(dimethylamino)ethyl methacrylate