title: Cigarette smoke-induced oxidative stress in skeletal muscles of mice.
abstract: Cigarette smoke (CS)-induced oxidative stress may cause muscle alterations in chronic conditions such as chronic obstructive pulmonary disease (COPD). We sought to explore in AKR/J mice exposed to CS for 6 months and in control animals, levels of protein oxidation, oxidized proteins (immunoblotting, proteomics) and antioxidant mechanisms in both respiratory and limb muscles, body weight modifications, systemic inflammation, and lung structure. Compared to control mice, CS-exposed animals exhibited a reduction in body weight gain at 3 months and thereafter, showed lung emphysema, and exhibited increased oxidative stress levels in their diaphragms and gastrocnemius at 6 months. Proteins involved in glycolysis, ATP production and distribution, carbon dioxide hydration, and muscle contraction were carbonylated in respiratory and limb muscles. Blood tumor necrosis factor (TNF)-alpha levels were significantly greater in CS-exposed mice than in control animals. In AKR/J mice, chronic exposure to CS induces lung emphysema concomitantly with greater oxidative modifications on muscle proteins in both respiratory and limb muscles, and systemic inflammation.
authors.affiliation: Pulmonology Department-Muscle and Respiratory System Research Unit, URMAR, IMIM-Hospital del Mar, Health and Experimental Sciences Department, Universitat Pompeu Fabra, Barcelona, Spain. ebarreiro@imim.es
authors.forename: Esther
authors.initials: E
authors.lastname: Barreiro
authors.forename: Laura
authors.initials: L
authors.lastname: del Puerto-Nevado
authors.forename: Ester
authors.initials: E
authors.lastname: Puig-Vilanova
authors.forename: Sandra
authors.initials: S
authors.lastname: Pérez-Rial
authors.forename: Francisco
authors.initials: F
authors.lastname: Sánchez
authors.forename: Lourdes
authors.initials: L
authors.lastname: Martínez-Galán
authors.forename: Stephanie
authors.initials: S
authors.lastname: Rivera
authors.forename: Joaquim
authors.initials: J
authors.lastname: Gea
authors.forename: Nicolás
authors.initials: N
authors.lastname: González-Mangado
authors.forename: Germán
authors.initials: G
authors.lastname: Peces-Barba
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism; D008297:Male; D051379:Mice; D008806:Mice, Inbred AKR; D009124:Muscle Proteins / Q000187:drug effects* / Q000378:metabolism; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D020543:Proteome / Q000187:drug effects / Q000378:metabolism; D011656:Pulmonary Emphysema / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D012906:Smoke; D012907:Smoking / Q000009:adverse effects*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D009124:Muscle Proteins; D020543:Proteome; D012906:Smoke; D014409:Tumor Necrosis Factor-alpha