title: JUNGBRUNNEN1, a reactive oxygen species-responsive NAC transcription factor, regulates longevity in Arabidopsis.
abstract: The transition from juvenility through maturation to senescence is a complex process that involves the regulation of longevity. Here, we identify JUNGBRUNNEN1 (JUB1), a hydrogen peroxide (H(2)O(2))-induced NAC transcription factor, as a central longevity regulator in Arabidopsis thaliana. JUB1 overexpression strongly delays senescence, dampens intracellular H(2)O(2) levels, and enhances tolerance to various abiotic stresses, whereas in jub1-1 knockdown plants, precocious senescence and lowered abiotic stress tolerance are observed. A JUB1 binding site containing a RRYGCCGT core sequence is present in the promoter of DREB2A, which plays an important role in abiotic stress responses. JUB1 transactivates DREB2A expression in mesophyll cell protoplasts and transgenic plants and binds directly to the DREB2A promoter. Transcriptome profiling of JUB1 overexpressors revealed elevated expression of several reactive oxygen species-responsive genes, including heat shock protein and glutathione S-transferase genes, whose expression is further induced by H(2)O(2) treatment. Metabolite profiling identified elevated Pro and trehalose levels in JUB1 overexpressors, in accordance with their enhanced abiotic stress tolerance. We suggest that JUB1 constitutes a central regulator of a finely tuned control system that modulates cellular H(2)O(2) level and primes the plants for upcoming stress through a gene regulatory network that involves DREB2A.
authors.affiliation: University of Potsdam, Institute of Biochemistry and Biology, 14476 Potsdam-Golm, Germany.
authors.forename: Anhui
authors.initials: A
authors.lastname: Wu
authors.forename: Annapurna Devi
authors.initials: AD
authors.lastname: Allu
authors.forename: Prashanth
authors.initials: P
authors.lastname: Garapati
authors.forename: Hamad
authors.initials: H
authors.lastname: Siddiqui
authors.forename: Hakan
authors.initials: H
authors.lastname: Dortay
authors.forename: Maria-Inés
authors.initials: MI
authors.lastname: Zanor
authors.forename: Maria Amparo
authors.initials: MA
authors.lastname: Asensi-Fabado
authors.forename: Sergi
authors.initials: S
authors.lastname: Munné-Bosch
authors.forename: Carla
authors.initials: C
authors.lastname: Antonio
authors.forename: Takayuki
authors.initials: T
authors.lastname: Tohge
authors.forename: Alisdair R
authors.initials: AR
authors.lastname: Fernie
authors.forename: Kerstin
authors.initials: K
authors.lastname: Kaufmann
authors.forename: Gang-Ping
authors.initials: GP
authors.lastname: Xue
authors.forename: Bernd
authors.initials: B
authors.lastname: Mueller-Roeber
authors.forename: Salma
authors.initials: S
authors.lastname: Balazadeh
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D018506:Gene Expression Regulation, Plant; D055785:Gene Knockdown Techniques; D053263:Gene Regulatory Networks; D006861:Hydrogen Peroxide / Q000494:pharmacology; D055442:Metabolome; D016254:Mutagenesis, Insertional; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D011401:Promoter Regions, Genetic; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013312:Stress, Physiological; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D059467:Transcriptome
chemical_list: D029681:Arabidopsis Proteins; C114579:DREB2A protein, Arabidopsis; D017382:Reactive Oxygen Species; D014157:Transcription Factors; D006861:Hydrogen Peroxide