title: The human lung adenocarcinoma cell line EKVX produces an infectious xenotropic murine leukemia virus.
abstract: The cell lines of the NCI-60 panel represent different cancer types and have been widely utilized for drug screening and molecular target identification. Screening these cell lines for envelope proteins or gene sequences related to xenotropic murine leukemia viruses (X-MLVs) revealed that one cell line, EKVX, was a candidate for production of an infectious gammaretrovirus. The presence of a retrovirus infectious to human cells was confirmed by the cell-free transmission of infection to the human prostate cancer cell line LNCaP. Amplification and sequencing of additional proviral sequences from EKVX confirmed a high degree of similarity to X-MLV. The cell line EKVX was established following passage of the original tumor cells through nude mice, providing a possible source of the X-MLV found in the EKVX cells.
authors.affiliation: Laboratory of Cancer Prevention, National Cancer Institute-Frederick, Frederick, MD 21702, USA. cmarikj@mail.nih.gov
authors.forename: Joan L
authors.initials: JL
authors.lastname: Cmarik
authors.forename: Jami A
authors.initials: JA
authors.lastname: Troxler
authors.forename: Charlotte A
authors.initials: CA
authors.lastname: Hanson
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhang
authors.forename: Sandra K
authors.initials: SK
authors.lastname: Ruscetti
mesh_terms: D000230:Adenocarcinoma / Q000821:virology*; D000818:Animals; D001483:Base Sequence; D045744:Cell Line, Tumor; D005814:Genes, Viral / Q000235:genetics; D006801:Humans; D015151:Immunoblotting; D009052:Leukemia Virus, Murine / Q000235:genetics / Q000378:metabolism*; D007942:Leukemia, Experimental / Q000821:virology; D008175:Lung Neoplasms / Q000821:virology*; D051379:Mice; D008819:Mice, Nude / Q000821:virology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012192:Retroviridae Infections / Q000821:virology; D016415:Sequence Alignment; D014412:Tumor Virus Infections / Q000821:virology; D014759:Viral Envelope Proteins / Q000235:genetics
chemical_list: D014759:Viral Envelope Proteins