title: [Effects of different applying methods of mechanical stress on human bladder smooth muscle cells].
abstract: OBJECTIVE
  To investigate the mechanical stress of four different mechanical bioreactors on the proliferation of in vitro human bladder smooth muscle cells (hBSMCs) and to design a feasible stress applying device.
  
  
  METHODS
  We designed four mechanical bioreactors successively: Pressure Circular Tank, Strain Tank, Water Column and Pressure Culture Flask. hBSMCs were cultured under 40 cm H2O sustained hydrostatic pressure or 7.7 N-strian (pressure test group), and the controls were cultured without pressure. Cell survival and proliferation status of hBSMCs after the applying of mechanical stress were evaluated by the using of CCK8 assay, immunofluorescence staining cytoskeleton alpha-actin and RT-PCR detected PCNA mRNA.
  
  
  RESULTS
  There were no significance differences of CCK8 assay, immunofluorescence staining cytoskeleton alpha-actin and RT-PCR detected PCNA mRNA between pressure group and control group (P>0.05), and also no differences among the four pressure groups (P>0.05).
  
  
  CONCLUSIONS
  The four mechanical bioreactors are no toxic to in vitro human bladder smooth muscle cells. The 40 cmH2O hydrostatic pressure or 7.7 N-strian can not induce the proliferation of hBSMC.
authors.affiliation: Department of Urology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wu
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Xin
authors.initials: X
authors.lastname: Wei
authors.forename: Kun-jie
authors.initials: KJ
authors.lastname: Wang
mesh_terms: D049109:Cell Proliferation; D002478:Cells, Cultured; D006801:Humans; D006874:Hydrostatic Pressure; D032389:Myocytes, Smooth Muscle / Q000166:cytology*; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013314:Stress, Mechanical; D001743:Urinary Bladder / Q000166:cytology* / Q000502:physiology
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D012333:RNA, Messenger