title: Electronic barcoding of a viral gene at the single-molecule level.
abstract: A new single-molecule approach for rapid and purely electronic discrimination among similar genes is presented. Combining solid-state nanopores and γ-modified synthetic peptide nucleic acid probes, we accurately barcode genes by counting the number of probes attached to each gene and measuring their relative spacing. We illustrate our method by sensing individual genes from two highly similar human immunodeficiency virus subtypes, demonstrating feasibility of a novel, single-molecule diagnostic platform for rapid pathogen classification.
authors.affiliation: Department of Biomedical Engineering, Boston University, Boston, Massachusetts 02215, USA.
authors.forename: Alon
authors.initials: A
authors.lastname: Singer
authors.forename: Srinivas
authors.initials: S
authors.lastname: Rapireddy
authors.forename: Danith H
authors.initials: DH
authors.lastname: Ly
authors.forename: Amit
authors.initials: A
authors.lastname: Meller
mesh_terms: D058893:DNA Barcoding, Taxonomic / Q000379:methods*; D036103:Nanotechnology / Q000379:methods*; D014764:Viral Proteins / Q000032:analysis* / Q000235:genetics*
chemical_list: D014764:Viral Proteins