title: A highly selective and sensitive in vivo fluorosensor for zinc(II) without cytotoxicity.
abstract: A highly selective and sensitive fluorescent Zn(2+) sensor, 2,6-bis(2-hydroxy-benzoic acid hydrazide)-4-methylphenol (1), was designed and synthesized. In aqueous THF (4 : 6 v/v) ligand 1 induces a 2 : 1 complex formation with respect to Zn(2+) at physiological pH. This probe features visible light excitation(390 nm) and emission (490 nm) profiles, excellent selectivity responses for Zn(2+)over other competing biological metal ions with K(d) < 1 pM(2), LOD < 1 ng L(-1) and about 680 fold enhancement in fluorescent intensity upon Zn(2+) binding. It also exhibits cell permeability and intracellular Zn(2+) sensing in A375 human melanoma cancer cell.
authors.affiliation: Department of Chemistry, Jadavpur University, Kolkata 700 032, India.
authors.forename: Tarun
authors.initials: T
authors.lastname: Mistri
authors.forename: Malay
authors.initials: M
authors.lastname: Dolai
authors.forename: Debrup
authors.initials: D
authors.lastname: Chakraborty
authors.forename: Anisur Rahman
authors.initials: AR
authors.lastname: Khuda-Bukhsh
authors.forename: Kalyan Kumar
authors.initials: KK
authors.lastname: Das
authors.forename: Mahammad
authors.initials: M
authors.lastname: Ali
mesh_terms: D045744:Cell Line, Tumor; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry*; D006801:Humans; D006835:Hydrazones / Q000032:analysis / Q000737:chemistry*; D008958:Models, Molecular; D015394:Molecular Structure; D010636:Phenols / Q000032:analysis / Q000737:chemistry*; D015032:Zinc / Q000032:analysis*
chemical_list: C572711:2,6-bis(2-hydroxybenzoic acid hydrazide)-4-methylphenol; D005456:Fluorescent Dyes; D006835:Hydrazones; D010636:Phenols; D015032:Zinc