title: Cationic carbosilane dendrimers-lipid membrane interactions.
abstract: The aim of this work was to study interactions between cationic carbosilane dendrimers (CBS) and lipid bilayers or monolayers. Two kinds of second generation carbosilane dendrimers were used: NN16 with Si-O bonds and BDBR0011 with Si-C bonds. The results show that cationic carbosilane dendrimers interact both with liposomes and lipid monolayers. Interactions were stronger for negatively charged membranes and high concentration of dendrimers. In liposomes interactions were studied by measuring fluorescence anisotropy changes of fluorescent labels incorporated into the bilayer. An increase in fluorescence anisotropy was observed for both fluorescent probes when dendrimers were added to lipids that means the decreased membrane fluidity. Both the hydrophobic and hydrophilic parts of liposome bilayers became more rigid. This may be due to dendrimers' incorporation into liposome bilayer. For higher concentrations of both dendrimers precipitation occurred in negatively charged liposomes. NN16 dendrimer interacted stronger with hydrophilic part of bilayers whereas BDBR0011 greatly modified the hydrophobic area. Monolayers method brought similar results. Both dendrimers influenced lipid monolayers and changed surface pressure. For negatively charged lipids the monitored parameter changed stronger than for uncharged DMPC lipids. Moreover, NN16 dendrimer interacted stronger than the BDBR0011.
authors.affiliation: Department of General Biophysics, University of Lodz, Poland. domwro@biol.uni.lodz.pl
authors.forename: Dominika
authors.initials: D
authors.lastname: Wrobel
authors.forename: Arkadiusz
authors.initials: A
authors.lastname: Kłys
authors.forename: Maksim
authors.initials: M
authors.lastname: Ionov
authors.forename: Pavol
authors.initials: P
authors.lastname: Vitovic
authors.forename: Iveta
authors.initials: I
authors.lastname: Waczulikowa
authors.forename: Tibor
authors.initials: T
authors.lastname: Hianik
authors.forename: Rafael
authors.initials: R
authors.lastname: Gomez-Ramirez
authors.forename: Javier
authors.initials: J
authors.lastname: de la Mata
authors.forename: Barbara
authors.initials: B
authors.lastname: Klajnert
authors.forename: Maria
authors.initials: M
authors.lastname: Bryszewska
mesh_terms: D002412:Cations / Q000737:chemistry; D050091:Dendrimers / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D008051:Lipid Bilayers / Q000737:chemistry*; D008560:Membrane Fluidity; D012821:Silanes / Q000737:chemistry*; D055672:Static Electricity
chemical_list: D002412:Cations; D050091:Dendrimers; D008051:Lipid Bilayers; D012821:Silanes; C504072:carbosilane