title: Transdermal administration of lactoferrin with sophorolipid.
abstract: Lactoferrin (Lf), a multifunctional glycoprotein, is known to activate dermal fibroblasts. Enhancing percutaneous absorption without decreasing the activity of Lf is critical in making the dermal administration of Lf beneficial. Sophorolipid (SL), a glycolipid-type biosurfactant, is known to form assemblies that may elevate the efficiency of the transdermal delivery of active ingredients. Here, we investigated the role of SL in the transdermal absorption of bovine Lf (bLf) and the effect of SL on the bLf activity on dermal fibroblasts. Transdermal absorption of bLf through a model skin was enhanced by 1.3-fold to 1.7-fold when SL was added. The effects of SL on the bLf activities on dermal fibroblasts were examined by cell proliferation activities and by gene expression levels of elastic fiber components, collagen IV, and hyaluronan synthases, revealing that SL did not depress the effect of bLf to any extent. Instead, the tropoelastin gene expression was upregulated ~60-fold by bLf alone, which was further increased to ~160-fold by bLf and SL together, suggesting a significant synergism between bLf and SL. Protein levels of elastin, assessed by immunohistochemistry, correlated well with the results of gene expressions. These results indicate the feasibility of the transdermal administration of bLf with SL.
authors.affiliation: Biochemical Laboratory, Saraya Co. Ltd., 24-12 Tamate, Kashiwara, Osaka 582-0028, Japan.
authors.forename: Nanase
authors.initials: N
authors.lastname: Ishii
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Kentaro
authors.initials: K
authors.lastname: Matsumiya
authors.forename: Mizuyuki
authors.initials: M
authors.lastname: Ryu
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Hirata
authors.forename: Yasuki
authors.initials: Y
authors.lastname: Matsumura
authors.forename: Yasushi A
authors.initials: YA
authors.lastname: Suzuki
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D004337:Drug Carriers / Q000008:administration & dosage*; D004549:Elastin / Q000235:genetics / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D006017:Glycolipids / Q000008:administration & dosage*; D006801:Humans; D007781:Lactoferrin / Q000008:administration & dosage* / Q000378:metabolism / Q000494:pharmacology; D008567:Membranes, Artificial; D008954:Models, Biological; D010539:Permeability; D012867:Skin / Q000166:cytology; D013501:Surface-Active Agents / Q000008:administration & dosage*; D014333:Tropoelastin / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D004337:Drug Carriers; D006017:Glycolipids; D008567:Membranes, Artificial; D013501:Surface-Active Agents; D014333:Tropoelastin; D004549:Elastin; D007781:Lactoferrin