title: Analysis of ethanol in fermentation samples by a robust nanocomposite-based microbial biosensor.
abstract: A robust microbial biosensor was constructed from a bionanocomposite prepared by a direct mixing of bacterial cells of Gluconobacter oxydans and carbon nanotubes with ferricyanide employed as a mediator for enhanced sensitivity of ethanol oxidation. A successful integration of the device into flow injection analysis mode of operation provided a high sensitivity of detection of (74 ± 2.7) μA mM(-1) cm(-2), a low detection limit of 5 μM and a linear range from 10 μM up to 1 mM. A short response time of the biosensor allowed a sample throughput of 67 h(-1) at 0.3 ml min(-1). The biosensor exhibited high operational stability with a decrease in the biosensor response of 1.7% during 43 h of continuous operation. The device was used to analyse ethanol in fermentation samples with a good agreement with a HPLC method.
authors.affiliation: Department of Glycobiotechnology, Institute of Chemistry, Slovak Academy of Sciences, Bratislava, SK, Slovakia.
authors.forename: Jana
authors.initials: J
authors.lastname: Sefčovičová
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Filip
authors.forename: Vladimír
authors.initials: V
authors.lastname: Mastihuba
authors.forename: Peter
authors.initials: P
authors.lastname: Gemeiner
authors.forename: Jan
authors.initials: J
authors.lastname: Tkac
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D000431:Ethanol / Q000032:analysis*; D005285:Fermentation; D005292:Ferricyanides / Q000737:chemistry; D020600:Gluconobacter oxydans / Q000378:metabolism*; D053761:Nanocomposites / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry; D012680:Sensitivity and Specificity
chemical_list: D005292:Ferricyanides; D037742:Nanotubes, Carbon; C007931:hexacyanoferrate III; D000431:Ethanol