title: Distribution of CD4+ CD25 high FoxP3+ regulatory T-cells in umbilical cord blood.
abstract: OBJECTIVE
  This study aimed to establish reference intervals for lymphocyte subsets including CD4(+)CD25(high)FoxP3(+) regulatory T-cells (Tregs) in umbilical cord blood.
  
  
  METHODS
  Umbilical cord blood was obtained after birth from 120 healthy full-term neonates, who were born between November 2010 and November 2011. Lymphocyte subsets including Tregs were analysed using flow cytometer (Beckman Coulter, Fullerton, CA, USA), and the reference intervals were defined using non-parametrical percentile methods according to the Clinical and Laboratory Standard Institute guideline (C28-A3).
  
  
  RESULTS
  The reference intervals for lymphocyte subsets were: helper T-cells (CD3(+)/CD4(+)), 15.40-70.06%; cytotoxic T-cells (CD3(+)/CD8(+)), 9.65-34.28%; B-cells (CD19(+)), 4.50-29.59%; and natural killer cells (CD3(-)/CD16(+)/CD56(+)), 1.42-28.03%. The reference interval for Tregs was 0.35-9.07%.
  
  
  CONCLUSIONS
  This study provides the reference intervals for lymphocyte subsets including Tregs in umbilical cord blood from healthy full-term neonates. These results could be used as fundamental data for clinical laboratory tests as well as future researches.
authors.affiliation: Department of Laboratory Medicine, Konkuk University School of Medicine, Seoul, Korea.
authors.forename: Hanah
authors.initials: H
authors.lastname: Kim
authors.forename: Hee-Won
authors.initials: HW
authors.lastname: Moon
authors.forename: Mina
authors.initials: M
authors.lastname: Hur
authors.forename: Chul-Min
authors.initials: CM
authors.lastname: Park
authors.forename: Yeo-Min
authors.initials: YM
authors.lastname: Yun
authors.forename: Han Sung
authors.initials: HS
authors.lastname: Hwang
authors.forename: Han Sung
authors.initials: HS
authors.lastname: Kwon
authors.forename: In Sook
authors.initials: IS
authors.lastname: Sohn
mesh_terms: D016175:B-Lymphocyte Subsets / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D015704:CD4 Antigens / Q000378:metabolism; D005312:Fetal Blood / Q000276:immunology*; D005434:Flow Cytometry; D051858:Forkhead Transcription Factors / Q000378:metabolism; D006801:Humans; D007231:Infant, Newborn; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism; D007694:Killer Cells, Natural / Q000378:metabolism; D018655:Lymphocyte Count; D012016:Reference Values; D013602:T-Lymphocytes, Cytotoxic / Q000378:metabolism; D006377:T-Lymphocytes, Helper-Inducer / Q000378:metabolism; D050378:T-Lymphocytes, Regulatory / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D015704:CD4 Antigens; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D053645:Interleukin-2 Receptor alpha Subunit