title: Evaluation of NK-to-target cell binding and evidence for T cell conjugates by flow cytometry.
abstract: A flow cytometric methodology was set up to assess the binding capability of peripheral blood NK and T cells to the K562 tumor cell line. Differential side scatter characteristics between effectors and targets were used to analyze conjugated and unconjugated cells. The previous labeling of NK and T cells with anti-Leu 11c and anti-Leu 4 monoclonal antibodies, allowed the distinction between unconjugated non-fluorescent and conjugated fluorescent targets and the percentual evaluation of bound anti-Leu 11c(+) and anti-Leu 4(+) cells.
authors.affiliation: Istituto di Anatomia Umana Normale, Bologna, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Vitale
authors.forename: R
authors.initials: R
authors.lastname: Rizzoli
authors.forename: A R
authors.initials: AR
authors.lastname: Mariani
authors.forename: L M
authors.initials: LM
authors.lastname: Neri
authors.forename: A
authors.initials: A
authors.lastname: Facchini
authors.forename: S
authors.initials: S
authors.lastname: Papa