title: The soluble proteome of tobacco Bright Yellow-2 cells undergoing H₂O₂-induced programmed cell death.
abstract: Plant programmed cell death (PCD) is a genetically controlled process that plays an important role in development and stress responses. Reactive oxygen species (ROS) are key inducers of PCD. The addition of 50 mM H₂O₂ to tobacco Bright Yellow-2 (TBY-2) cell cultures induces PCD. A comparative proteomic analysis of TBY-2 cells treated with 50 mM H₂O₂ for 30 min and 3 h was performed. The results showed early down-regulation of several elements in the cellular redox hub and inhibition of the protein repair-degradation system. The expression patterns of proteins involved in the homeostatic response, in particular those associated with metabolism, were consistently altered. The changes in abundance of several cytoskeleton proteins confirmed the active role of the cytoskeleton in PCD signalling. Cells undergoing H₂O₂-induced PCD fail to cope with oxidative stress. The antioxidant defence system and the anti-PCD signalling cascades are inhibited. This promotes a genetically programmed cell suicide pathway. Fifteen differentially expressed proteins showed an expression pattern similar to that previously observed in TBY-2 cells undergoing heat shock-induced PCD. The possibility that these proteins are part of a core complex required for PCD induction is discussed.
authors.affiliation: Dipartimento Biotecnologie e Scienze della Vita, Università degli Studi dell'Insubria, Via J.H. Dunant 3, 21100 Varese, Italy.
authors.forename: Candida
authors.initials: C
authors.lastname: Vannini
authors.forename: Milena
authors.initials: M
authors.lastname: Marsoni
authors.forename: Carlo
authors.initials: C
authors.lastname: Cantara
authors.forename: Maria Concetta
authors.initials: MC
authors.lastname: De Pinto
authors.forename: Vittoria
authors.initials: V
authors.lastname: Locato
authors.forename: Laura
authors.initials: L
authors.lastname: De Gara
authors.forename: Marcella
authors.initials: M
authors.lastname: Bracale
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D060387:Ascorbate Peroxidases / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D016000:Cluster Analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D006706:Homeostasis / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D010084:Oxidation-Reduction / Q000187:drug effects; D010940:Plant Proteins / Q000378:metabolism; D014176:Protein Biosynthesis / Q000187:drug effects; D059748:Proteolysis / Q000187:drug effects; D020543:Proteome / Q000378:metabolism*; D012995:Solubility / Q000187:drug effects; D013095:Spermidine / Q000494:pharmacology; D014026:Nicotiana / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D020543:Proteome; D006861:Hydrogen Peroxide; D060387:Ascorbate Peroxidases; D013095:Spermidine