title: Immunophenotypic analysis of myeloperoxidase-negative leukemia cutis and blastic plasmacytoid dendritic cell neoplasm.
abstract: Myeloid leukemia cutis (LC) and blastic plasmacytoid dendritic cell neoplasm (BPDCN) are morphologically indistinguishable malignancies that frequently manifest in the skin. Separating myeloperoxidase-negative LC from BPDCN may be particularly challenging. We identified a panel of immunohistochemical stains to distinguish myeloid LC (23 cases) from BPDCN (12 cases): myeloperoxidase, which stained 7 cases (30%) of LC and 0 cases (0%) of BPDCN; CD56, which stained 12 cases (52%) of LC and all 12 cases (100%) of BPDCN; CD4, which stained 2 cases (9%) of LC and all 12 cases (100%) of BPDCN; CD123, which stained 4 cases (17%) of LC and 10 cases (83%) of BPDCN; and Tcl-1, which stained 2 cases (9%) of LC and 9 (82%) of 11 cases of BPDCN. It is interesting that CD33 was not helpful; it stained 18 (78%) cases of LC and 11 cases (92%) of BPDCN. Our results indicate that a panel that includes CD4, CD56, CD123, and Tcl-1 can appropriately distinguish between these 2 entities.
authors.affiliation: Department of Pathology, Stanford University, Stanford, CA 94305, USA.
authors.forename: Danielle M P
authors.initials: DM
authors.lastname: Cronin
authors.forename: Tracy I
authors.initials: TI
authors.lastname: George
authors.forename: Kaaren K
authors.initials: KK
authors.lastname: Reichard
authors.forename: Uma N
authors.initials: UN
authors.lastname: Sundram
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism; D019046:Bone Marrow Neoplasms; D015704:CD4 Antigens; D019002:CD56 Antigen / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D003713:Dendritic Cells / Q000201:enzymology / Q000276:immunology / Q000473:pathology*; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D007223:Infant; D007231:Infant, Newborn; D053650:Interleukin-3 Receptor alpha Subunit / Q000378:metabolism; D007951:Leukemia, Myeloid / Q000175:diagnosis* / Q000201:enzymology / Q000276:immunology; D008297:Male; D008875:Middle Aged; D054219:Neoplasms, Plasma Cell / Q000175:diagnosis* / Q000201:enzymology / Q000276:immunology; D009195:Peroxidase / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D012878:Skin Neoplasms / Q000175:diagnosis* / Q000201:enzymology / Q000276:immunology; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D015704:CD4 Antigens; D019002:CD56 Antigen; D053650:Interleukin-3 Receptor alpha Subunit; D011518:Proto-Oncogene Proteins; C090851:TCL1A protein, human; D009195:Peroxidase