title: Simultaneous determination of six active components in traditional herbal medicine 'Oyaksungisan' by HPLC-DAD.
abstract: In this study, an effective high performance liquid chromatography-diode array detector (HPLC-DAD) method was established for simultaneous determination of six marker compounds, ephedrine hydrochloride, 6-gingerol, glycyrrhizin, hesperidin, imperatorin and ferulic acid, in a Korean traditional prescription, Oyaksungisan, which is used for hemiplegia, arthralgia and paralysis. The six marker compounds of Oyaksungisan were separated on a LUNA C18 column (S-5 μm, 4.6 mm I.D. × 250 mm) at a column temperature of 35°C. The gradient elution was composed of water with 0.1% trifluoroacetic acid and methanol. The detection UV wavelengths were set at 207, 250, 280 and 320 nm. Calibration curves for the six compounds showed good linear regressions (R (2) > 0.9999). The limits of detection and limits of quantification were within the ranges 0.003-0.01 and 0.01-0.04 μg/ml, respectively. The relative standard deviation (RSD) values of intra- and inter-day testing were within the ranges 0.10-1.82 and 0.04-1.59%, respectively. The results of the recovery test were 95.05-104.27% with a RSD value of 0.11-1.85%. In conclusion, the simultaneous determination method developed was useful in the quality evaluation of Oyaksungisan.
authors.affiliation: Division of Bioscience and Biotechnology, Department of Biomaterials Engineering, Kangwon National University, Chuncheon 200-701, Korea.
authors.forename: Jin Bae
authors.initials: JB
authors.lastname: Weon
authors.forename: Hye Jin
authors.initials: HJ
authors.lastname: Yang
authors.forename: Jin Yeul
authors.initials: JY
authors.lastname: Ma
authors.forename: Choong Je
authors.initials: CJ
authors.lastname: Ma
mesh_terms: D002396:Catechols / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003373:Coumaric Acids / Q000032:analysis; D004809:Ephedrine / Q000032:analysis; D005233:Fatty Alcohols / Q000032:analysis; D011564:Furocoumarins / Q000032:analysis; D019695:Glycyrrhizic Acid / Q000032:analysis; D029001:Herbal Medicine / Q000379:methods*; D006569:Hesperidin / Q000032:analysis; D055670:Medicine, Korean Traditional
chemical_list: D002396:Catechols; D003373:Coumaric Acids; D005233:Fatty Alcohols; D011564:Furocoumarins; D019695:Glycyrrhizic Acid; C007845:gingerol; C004999:ferulic acid; D006569:Hesperidin; D004809:Ephedrine; C031534:imperatorin