title: B23/nucleophosmin is involved in regulation of adenovirus chromatin structure at late infection stages, but not in virus replication and transcription.
abstract: B23/nucleophosmin has been identified in vitro as a stimulatory factor for replication of adenovirus DNA complexed with viral basic core proteins. In the present study, the in vivo function of B23 in the adenovirus life cycle was studied. It was found that both the expression of a decoy mutant derived from adenovirus core protein V that tightly associates with B23 and small interfering RNA-mediated depletion of B23 impeded the production of progeny virions. However, B23 depletion did not significantly affect the replication and transcription of the virus genome. Chromatin immunoprecipitation analyses revealed that B23 depletion significantly increased the association of viral DNA with viral core proteins and cellular histones. These results suggest that B23 is involved in the regulation of association and/or dissociation of core proteins and cellular histones with the virus genome. In addition, these results suggest that proper viral chromatin assembly, regulated in part by B23, is crucial for the maturation of infectious virus particles.
authors.affiliation: Department of Applied Nutrition and Food Technology, Faculty of Applied Science and Technology, Islamic University, Kushtia, Bangladesh.|Graduate School of Comprehensive Human Sciences and Faculty of Medicine, University of Tsukuba, 1-1-1 Tennohdai, Tsukuba 305-8575, Japan.
authors.forename: Mohammad Abdus
authors.initials: MA
authors.lastname: Samad
authors.affiliation: Graduate School of Comprehensive Human Sciences and Faculty of Medicine, University of Tsukuba, 1-1-1 Tennohdai, Tsukuba 305-8575, Japan.
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Komatsu
authors.affiliation: Initiatives for the Promotion of Young Scientists' Independent Research, University of Tsukuba, 1-1-1 Tennohdai, Tsukuba 305-8577, Japan.|Graduate School of Comprehensive Human Sciences and Faculty of Medicine, University of Tsukuba, 1-1-1 Tennohdai, Tsukuba 305-8575, Japan.
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Okuwaki
authors.affiliation: Graduate School of Comprehensive Human Sciences and Faculty of Medicine, University of Tsukuba, 1-1-1 Tennohdai, Tsukuba 305-8575, Japan.
authors.forename: Kyosuke
authors.initials: K
authors.lastname: Nagata
mesh_terms: D000258:Adenovirus Infections, Human / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D000260:Adenoviruses, Human / Q000235:genetics / Q000254:growth & development* / Q000502:physiology; D015967:Gene Expression Regulation, Viral; D006367:HeLa Cells; D006657:Histones / Q000378:metabolism; D006801:Humans; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000090243:Nucleophosmin; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D014158:Transcription, Genetic; D014758:Viral Core Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D006657:Histones; C000716967:NPM1 protein, human; D009687:Nuclear Proteins; D014758:Viral Core Proteins; D000090243:Nucleophosmin