title: Standardizing immunophenotyping for the Human Immunology Project.
abstract: The heterogeneity in the healthy human immune system, and the immunological changes that portend various diseases, have been only partially described. Their comprehensive elucidation has been termed the 'Human Immunology Project'. The accurate measurement of variations in the human immune system requires precise and standardized assays to distinguish true biological changes from technical artefacts. Thus, to be successful, the Human Immunology Project will require standardized assays for immunophenotyping humans in health and disease. A major tool in this effort is flow cytometry, which remains highly variable with regard to sample handling, reagents, instrument setup and data analysis. In this Review, we outline the current state of standardization of flow cytometry assays and summarize the steps that are required to enable the Human Immunology Project.
authors.affiliation: Institute for Immunity, Transplantation and Infection, Stanford University School of Medicine, Stanford, California 94305, USA. maecker@stanford.edu
authors.forename: Holden T
authors.initials: HT
authors.lastname: Maecker
authors.forename: J Philip
authors.initials: JP
authors.lastname: McCoy
authors.forename: Robert
authors.initials: R
authors.lastname: Nussenblatt
mesh_terms: D000818:Animals; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods / Q000592:standards; D006801:Humans; D016130:Immunophenotyping / Q000295:instrumentation / Q000379:methods* / Q000592:standards*; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D051379:Mice; D012015:Reference Standards