title: Loss of Med1/TRAP220 promotes the invasion and metastasis of human non-small-cell lung cancer cells by modulating the expression of metastasis-related genes.
abstract: Med1/TRAP220 is an essential component of the TRAP/Mediator complex. In this study, we present a novel function of Med1 in human non-small-cell lung cancer (NSCLC) progression. We found that the loss of Med1 expression was strongly associated with increased rates of invasion and metastasis in NSCLC patients. Consistent with lung cancer patient data, the knockdown of Med1 in NSCLC cell lines led to an increase in cell migration and invasion. Med1-depleted cells displayed an increase in metastasis in a xenograft tumor model and in an in vivo metastasis assay. Moreover, a microarray analysis revealed that the mRNA levels of the metastasis-related genes uPAR, ID2, ID4, PTP4A1, PKP3, TGM2, PLD1, TIMP2, RGS2, and HOXA4 were altered upon Med1 knockdown. Collectively, these results suggest that the loss of Med1 increases the invasive potential of human NSCLC cells by modulating the expression of metastasis-related genes.
authors.affiliation: Department of Biochemistry, College of Medicine, Dong-A University, Busan 602-714, South Korea.
authors.forename: Hyun-Ju
authors.initials: HJ
authors.lastname: Kim
authors.forename: Mee Sook
authors.initials: MS
authors.lastname: Roh
authors.forename: Choon Hee
authors.initials: CH
authors.lastname: Son
authors.forename: Ae Jeong
authors.initials: AJ
authors.lastname: Kim
authors.forename: Hye Jin
authors.initials: HJ
authors.lastname: Jee
authors.forename: Naree
authors.initials: N
authors.lastname: Song
authors.forename: Minjee
authors.initials: M
authors.lastname: Kim
authors.forename: Su-Young
authors.initials: SY
authors.lastname: Seo
authors.forename: Young Hyun
authors.initials: YH
authors.lastname: Yoo
authors.forename: Jeanho
authors.initials: J
authors.lastname: Yun
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D015870:Gene Expression; D005786:Gene Expression Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D056913:Mediator Complex Subunit 1 / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D009361:Neoplasm Invasiveness / Q000235:genetics*; D009362:Neoplasm Metastasis / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: C538855:MED1 protein, human; D056913:Mediator Complex Subunit 1; D012333:RNA, Messenger; D014157:Transcription Factors