title: Fibrillation potentials of denervated rat skeletal muscle are associated with expression of cardiac-type voltage-gated sodium channel isoform Nav1.5.
abstract: OBJECTIVE
  The molecular mechanisms underlying fibrillation potentials are still unclear. We hypothesised that expression of the cardiac-type voltage-gated sodium channel isoform Nav1.5 in denervated rat skeletal muscle is associated with the generation of such potentials.
  
  
  METHODS
  Muscle samples were extracted and analysed biologically from surgically denervated rat extensor digitorum longus muscle after concentric needle electromyographic recording at various time points after denervation (4h to 6days).
  
  
  RESULTS
  Both nav1.5 messenger RNA (mRNA) signal on northern blotting and Nav1.5 protein expression on immunohistochemistry appeared on the second day after denervation, exactly when fibrillation potentials appeared. Administration of lidocaine, which has much stronger affinity for sodium channels in cardiac muscle than for those in skeletal muscle, dramatically decreased fibrillation potentials, but had no effect on contralateral compound muscle action potentials.
  
  
  CONCLUSIONS
  Expression of Nav1.5 participates in the generation of fibrillation potentials in denervated rat skeletal muscle.
  
  
  CONCLUSIONS
  We proposed an altered expression of voltage-gated sodium channel isoforms as a novel mechanism to explain the occurrence of fibrillation potentials following skeletal muscle denervation.
authors.affiliation: Department of Neurology, Kobe University Graduate School of Medicine, Kobe City, Japan. sekiguch@med.kobe-u.ac.jp
authors.forename: Kenji
authors.initials: K
authors.lastname: Sekiguchi
authors.forename: Fumio
authors.initials: F
authors.lastname: Kanda
authors.forename: Shigeru
authors.initials: S
authors.lastname: Mitsui
authors.forename: Nobuo
authors.initials: N
authors.lastname: Kohara
authors.forename: Kazuo
authors.initials: K
authors.lastname: Chihara
mesh_terms: D000818:Animals; D004576:Electromyography; D005260:Female; D008012:Lidocaine / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology*; D009121:Muscle Denervation; D018482:Muscle, Skeletal / Q000187:drug effects / Q000294:innervation / Q000378:metabolism*; D062554:NAV1.5 Voltage-Gated Sodium Channel; D051381:Rats; D017208:Rats, Wistar; D015222:Sodium Channels / Q000235:genetics / Q000378:metabolism*
chemical_list: D062554:NAV1.5 Voltage-Gated Sodium Channel; C568322:Scn5a protein, rat; D015222:Sodium Channels; D008012:Lidocaine