title: Optical clearing of porcine skin tissue in vitro studied by Raman microspectroscopy.
abstract: In present work, we studied the effect of optical clearing on porcine skin in vitro with glycerol by Raman microspectroscopy, denoted as RM, at various time intervals of 0, 15, 30, 45, 60, and 75 min respectively. The results showed that the addition of glycerol significantly improved the depth of RM measurement, and enhanced the recovery of skin tissue Raman spectra that were not overlapped with the glycerol Raman spectra over time. Moreover, it was found that the Raman signals resembled the native spectrum of the molecules in porcine skin with a negligible frequency shift. Furthermore, we evaluated the extent of optical clearing in porcine skin by utilizing various concentrations of 40%, 60%, and 80% glycerol solution. The results demonstrated that with the increase of concentration of glycerol, the optical clearing of porcine skin was much improved.
authors.affiliation: South China Normal University, Laboratory of Photonic Chinese Medicine, MOE Key Laboratory of Laser Life Science, College of Biophotonics, 510631, Guangzhou, Guangdong, China.
authors.forename: Deqiu
authors.initials: D
authors.lastname: Huang
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.forename: Huiqing
authors.initials: H
authors.lastname: Zhong
authors.forename: Honglian
authors.initials: H
authors.lastname: Xiong
authors.forename: Xi
authors.initials: X
authors.lastname: Guo
authors.forename: Zhouyi
authors.initials: Z
authors.lastname: Guo
mesh_terms: D000818:Animals; D005990:Glycerol / Q000737:chemistry; D012867:Skin / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013552:Swine
chemical_list: D005990:Glycerol