title: The Effect of Garlic Extract on Expression of INFγ And Inos Genes in Macrophages Infected with Leishmania major.
abstract: BACKGROUND
  The study was aimed to show the effect of molecular mechanism of Aqueous Garlic Extract (AGE) on expression of IFNγ and iNOS genes in Leishmania major.
  
  
  METHODS
  Leishmania major promastigotes (MRHO/IR/75/ER) were added to the in-vitro cultured J774 cell line, the cells were incubated for 72 hours. Various concentrations of garlic extract (9.25, 18.5, 37, 74, 148 mg/ml) were added to the infected cells. MTT assay was applied for cellular proliferation. After 72 hours of incubation, supernatants were collected and total RNA was extracted from the infected cells. The express of IFNγ and iNOS genes were studied by RT-PCR method.
  
  
  RESULTS
  The colorimetric MTT assay after 3 days of incubation showed cytotoxic effect of garlic extract with an IC50 of 37 mg/ml. In addition, IFNγ and iNOS genes expression by RT-PCR indicated that garlic extract lead to over expression of these genes in J774 cell line infected with L. major.
  
  
  CONCLUSIONS
  Garlic extract exerts cytotoxic effect on infected J774 cell line. In addition, the hypothesis that garlic can improve cellular immunity with raising the expression of IFNγ and of iNOS genes confirmed.
authors.affiliation: Department of Medical Parasitology, Tehran University of Medical Sciences, Tehran, Iran & Research Institute for Islamic & Complementary Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mj
authors.initials: M
authors.lastname: Gharavi
authors.forename: M
authors.initials: M
authors.lastname: Nobakht
authors.forename: Sh
authors.initials: Sh
authors.lastname: Khademvatan
authors.forename: E
authors.initials: E
authors.lastname: Bandani
authors.forename: M
authors.initials: M
authors.lastname: Bakhshayesh
authors.forename: M
authors.initials: M
authors.lastname: Roozbehani