title: Dual role of selected antioxidants found in dietary supplements: crossover between anti- and pro-oxidant activities in the presence of copper.
abstract: Overproduction of reactive oxygen species (ROS) in vivo can result in damage associated with many aging-associated diseases. Defenses against ROS that have evolved include antioxidant enzymes, such as superoxide dismutases, peroxidases, and catalases, which can scavenge ROS. In addition, endogenous and dietary antioxidants play an important role in moderating damage associated with ROS. In this study, we use four common dietary antioxidants to demonstrate that, in the presence of copper (cupric sulfate and cupric gluconate) and physiologically relevant levels of hydrogen peroxide, these antioxidants can also act as pro-oxidants by producing hydroxyl radicals. Using electron spin resonance (ESR) spin trapping techniques, we demonstrate that the level of hydroxyl radical formation is a function of the pH of the medium and the relative amounts of antioxidant and copper. On the basis of the level of hydroxyl radical formation, the relative pro-oxidant potential of these antioxidants is cysteine > ascorbate > EGCG > GSH. It has been reported that copper sequestered by protein ligands, as happens in vivo, loses its redox activity (diminishing/abolishing the formation of free radicals). However, in the presence of hydrogen peroxide, cysteine and GSH efficiently react with cupric sulfate sequestered with bovine serum albumin to generate hydroxyl radicals. Overall, the results demonstrate that in the presence of copper, endogenous and dietary antioxidants can also exhibit pro-oxidative activity.
authors.affiliation: Center for Food Safety and Applied Nutrition, US Food and Drug Administration, College Park, Maryland 20740, United States. junjie.yin@fda.hhs.gov
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Yin
authors.forename: Peter P
authors.initials: PP
authors.lastname: Fu
authors.forename: Herman
authors.initials: H
authors.lastname: Lutterodt
authors.forename: Yu-Ting
authors.initials: YT
authors.lastname: Zhou
authors.forename: William E
authors.initials: WE
authors.lastname: Antholine
authors.forename: Wayne
authors.initials: W
authors.lastname: Wamer
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D003300:Copper / Q000737:chemistry*; D019587:Dietary Supplements / Q000032:analysis*; D016877:Oxidants / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D016877:Oxidants; D003300:Copper