title: Rootstock control of scion transpiration and its acclimation to water deficit are controlled by different genes.
abstract: The stomatal control of transpiration is one of the major strategies by which plants cope with water stress. Here, we investigated the genetic architecture of the rootstock control of scion transpiration-related traits over a period of 3 yr. The rootstocks studied were full sibs from a controlled interspecific cross (Vitis vinifera cv. Cabernet Sauvignon × Vitis riparia cv. Gloire de Montpellier), onto which we grafted a single scion genotype. After 10 d without stress, the water supply was progressively limited over a period of 10 d, and a stable water deficit was then applied for 15 d. Transpiration rate was estimated daily and a mathematical curve was fitted to its response to water deficit intensity. We also determined δ(13) C values in leaves, transpiration efficiency and water extraction capacity. These traits were then analysed in a multienvironment (year and water status) quantitative trait locus (QTL) analysis. Quantitative trait loci, independent of year and water status, were detected for each trait. One genomic region was specifically implicated in the acclimation of scion transpiration induced by the rootstock. The QTLs identified colocalized with genes involved in water deficit responses, such as those relating to ABA and hydraulic regulation. Scion transpiration rate and its acclimation to water deficit are thus controlled genetically by the rootstock, through different genetic architectures.
authors.affiliation: Bordeaux Sciences Agro, ISVV, EGFV, UMR 1287, F-33175 Gradignan, France.|Univ. Bordeaux, ISVV, EGFV, UMR 1287, F-33140 Villenave d'Ornon, France.|INRA, ISVV, EGFV, UMR 1287, F-33140 Villenave d'Ornon, France.
authors.forename: Elisa
authors.initials: E
authors.lastname: Marguerit
authors.affiliation: INRA, UMR 1137 Ecologie et Ecophysiologie Forestières, 54280 Champenoux, France.|Université de Lorraine, UMR 1137 Ecologie et Ecophysiologie Forestières, 54506 Vandoeuvre-les-Nancy, France.
authors.forename: Oliver
authors.initials: O
authors.lastname: Brendel
authors.affiliation: INRA, UMR 759 Laboratoire d'Ecophysiologie des Plantes sous Stress Environnementaux, 34060 Montpellier, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Lebon
authors.affiliation: Bordeaux Sciences Agro, ISVV, EGFV, UMR 1287, F-33175 Gradignan, France.|Univ. Bordeaux, ISVV, EGFV, UMR 1287, F-33140 Villenave d'Ornon, France.|INRA, ISVV, EGFV, UMR 1287, F-33140 Villenave d'Ornon, France.
authors.forename: Cornelis
authors.initials: C
authors.lastname: Van Leeuwen
authors.affiliation: Bordeaux Sciences Agro, ISVV, EGFV, UMR 1287, F-33175 Gradignan, France.|Univ. Bordeaux, ISVV, EGFV, UMR 1287, F-33140 Villenave d'Ornon, France.|INRA, ISVV, EGFV, UMR 1287, F-33140 Villenave d'Ornon, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Ollat
mesh_terms: D000064:Acclimatization / Q000235:genetics*; D004777:Environment; D017343:Genes, Plant / Q000235:genetics*; D010641:Phenotype; D018517:Plant Roots / Q000235:genetics* / Q000502:physiology; D018526:Plant Transpiration / Q000235:genetics* / Q000502:physiology; D040641:Quantitative Trait Loci / Q000235:genetics; D019655:Quantitative Trait, Heritable; D027843:Vitis / Q000235:genetics* / Q000502:physiology*; D014867:Water / Q000502:physiology*
chemical_list: D014867:Water