title: Initial culture conditions affect the sensitivity of HepG2 cells to excessive mechanical agitation.
abstract: Hepatoma cells, HepG2, grew normally on microcarriers even at a relatively high agitation rate if sufficient time was allowed for cell attachment and adhesion. However, if a high agitation rate was applied shortly after initial cell attachment, the growth rate was retarded. This sensitivity to mechanical agitation appears to be dependent on the inoculation cell density.
authors.affiliation: Department of Biological Science and Engineering, Korea Advanced Institute of Science and Technology, Seoul, Korea.
authors.forename: J H
authors.initials: JH
authors.lastname: Kim
authors.forename: W S
authors.initials: WS
authors.lastname: Hu