title: Molecular characterization of a tandem-repeat galectin-9 (RuGlec9) from Korean rose bitterling (Rhodeus uyekii).
abstract: Galectin-9 is a b-galactoside-binding lectin that regulates many cellular functions, ranging from cell adhesion to pathogen recognition. We isolated and characterized the cDNA of tandem-repeat galectin-9 (RuGlec9) from the Korean rose bitterling (Rhodeus uyekii), an endemic Korean fish belonging to the Acheilognathinae subfamily of the Cyprinidae family. RuGlec9 cDNA is 1486 bp long and encodes a polypeptide of 323 amino acids containing two carbohydrate-recognition domains connected by a linker peptide. The deduced amino acid sequence of RuGlec9 shows 45-84% amino acid sequence identity to other galectin-9 sequences, including those from mammals and fish. RuGlec9 appeared in a large cluster with other galectin-9 sequences from fish and is more closely related to galectin-9 from Danio rerio than to those of other fish and mammals. RuGlec9 mRNA was expressed highly in the testis, spleen, intestine, stomach, and liver, and moderately in the brain, kidney, ovary, and gills of normal Korean rose bitterling. RuGlec9 mRNA expression in the spleen was increased by lipopolysaccharide. These results suggest that RuGlec9 plays a role in innate immunity in Korean rose bitterling.
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute, Gijang-gun, Busan, Republic of Korea. konghj@nfrdi.go.kr
authors.forename: Hee Jeong
authors.initials: HJ
authors.lastname: Kong
authors.forename: Woo-Jin
authors.initials: WJ
authors.lastname: Kim
authors.forename: Hyung Soo
authors.initials: HS
authors.lastname: Kim
authors.forename: Ye Ji
authors.initials: YJ
authors.lastname: Lee
authors.forename: Chi Hong
authors.initials: CH
authors.lastname: Kim
authors.forename: Bo-Hye
authors.initials: BH
authors.lastname: Nam
authors.forename: Young-Ok
authors.initials: YO
authors.lastname: Kim
authors.forename: Dong-Gyun
authors.initials: DG
authors.lastname: Kim
authors.forename: Sang-Jun
authors.initials: SJ
authors.lastname: Lee
authors.forename: Sang-Gu
authors.initials: SG
authors.lastname: Lim
authors.forename: Bong-Seok
authors.initials: BS
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003530:Cyprinidae / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D020224:Expressed Sequence Tags; D037161:Galectins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008099:Liver / Q000378:metabolism; D008969:Molecular Sequence Data; D009132:Muscles / Q000378:metabolism; D010802:Phylogeny; D012333:RNA, Messenger / Q000737:chemistry; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012475:Salmonella / Q000502:physiology; D016415:Sequence Alignment; D013154:Spleen / Q000378:metabolism; D020080:Tandem Repeat Sequences; D014018:Tissue Distribution
chemical_list: D018076:DNA, Complementary; D037161:Galectins; D008070:Lipopolysaccharides; D012333:RNA, Messenger