title: Identification of envelope protein orf10 of channel catfish herpesvirus.
abstract: Channel catfish virus (CCV) is a viral pathogen of fry and fingerling channel catfish and can cause significant commercial loss. Previous studies have shown that the CCV virion contains at least 25 predicted structural proteins, including viral protein 10, which is encoded by the orf10 gene of the CCV. In this paper, the orf10 gene was expressed in Escherichia coli and used to produce a specific antibody. Western blot analysis confirmed that open reading frame 10 is an envelope protein. A viral neutralization assay demonstrated that open reading frame 10 antiserum was able to inhibit CCV infection of channel catfish ovary cells, suggesting that viral protein 10 is likely to play an important role in the CCV infection of channel catfish ovary cells.
authors.affiliation: College of Fisheries, Huazhong Agriculture University, Wuhan 430070, People's Republic of China.
authors.forename: Yulin
authors.initials: Y
authors.lastname: Liu
authors.forename: Junfa
authors.initials: J
authors.lastname: Yuan
authors.forename: Weimin
authors.initials: W
authors.lastname: Wang
authors.forename: Xiaoxuan
authors.initials: X
authors.lastname: Chen
authors.forename: Rong
authors.initials: R
authors.lastname: Tang
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Lijuan
authors.initials: L
authors.lastname: Li
mesh_terms: D000818:Animals; D002460:Cell Line; D004926:Escherichia coli / Q000235:genetics; D015686:Gene Products, env / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D052656:Ictalurivirus / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D007106:Immune Sera / Q000276:immunology; D016253:Microscopy, Immunoelectron; D009500:Neutralization Tests; D016366:Open Reading Frames / Q000235:genetics; D014771:Virion / Q000378:metabolism
chemical_list: D015686:Gene Products, env; D007106:Immune Sera