title: Development of microsatellite primers of the largest seagrass, Enhalus acoroides (Hydrocharitaceae).
abstract: OBJECTIVE
  Microsatellite primers were developed for the seagrass Enhalus acoroides to investigate genetic variation and identify clonal structure.
  
  
  RESULTS
  Four polymorphic loci and 32 monomorphic loci were developed in E. acoroides. Two to four alleles per locus were observed at the polymorphic loci across 60 individuals of two E. acoroides populations. The observed and expected heterozygosities within populations ranged from 0.100 to 0.5667 and from 0.0977 to 0.5079, respectively.
  
  
  CONCLUSIONS
  Our study revealed very low polymorphism in E. acoroides, even at the polymorphic loci. Nevertheless, these primers are a useful tool to study genetic variation, clonal structure, and mating system.
authors.affiliation: Department of Environmental Sciences, East China Normal University, Shanghai 200062, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Gao
authors.forename: Kai
authors.initials: K
authors.lastname: Jiang
authors.forename: Yan
authors.initials: Y
authors.lastname: Geng
authors.forename: Xiao-Yong
authors.initials: XY
authors.lastname: Chen
mesh_terms: D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D031321:Hydrocharitaceae / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D011110:Polymorphism, Genetic
chemical_list: D017931:DNA Primers; D018744:DNA, Plant