title: Characterizing the structural diversity of a bacterial community associated with filter materials in recirculating aquaculture systems of  Scortum barcoo.
abstract: The bacterial community structure associated with filter materials in the recirculating aquaculture system of  Scortum barcoo  was investigated using the 16S rRNA gene clone library method. Preliminary results showed that the clone library constructed from the initial operation condition was characterized by 31 taxa of bacteria belonging to eight phyla including Proteobacteria, Acidobacteria, Firmicutes, Fusobacteria, Sphingobacteria, Bacteroidetes, Verrucomicrobiae, and Actinobacteria. There were 14 taxa of bacteria belonging to four phyla including Proteobacteria, Acidobacteria, Planctomycetacia, and Nitrospirae from the stable operation condition where the water quality was well maintained. Nitrospirae was only found under the stable operation condition in this study. Our results further indicated that  Nitrospira  was dominated by members of the Nitrospira sp. lineages, with a minor fraction related to  Nitrospira moscoviensis  and an unknown Nitrospira cluster. These great differences of both diversity and composition between two operation conditions suggested that the composition of the microbial community varied with the degree of water quality in the recirculating aquaculture system of S. barcoo.
authors.affiliation: Key Laboratory of Applied Marine Biotechnology, (Ningbo University), Ministry of Education, 818 Fenghua Road, Ningbo, Zhejiang 315211, People's Republic of China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhu
authors.forename: Yangfang
authors.initials: Y
authors.lastname: Ye
authors.forename: Fangfang
authors.initials: F
authors.lastname: Pei
authors.forename: Kaihong
authors.initials: K
authors.lastname: Lu
mesh_terms: D000818:Animals; D017756:Aquaculture / Q000295:instrumentation; D001419:Bacteria / Q000145:classification / Q000235:genetics; D018407:Bacterial Physiological Phenomena; D044822:Biodiversity; D005374:Filtration / Q000295:instrumentation; D008969:Molecular Sequence Data; D010473:Perciformes; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014867:Water / Q000737:chemistry; D014871:Water Microbiology; D060753:Water Quality
chemical_list: D012336:RNA, Ribosomal, 16S; D014867:Water