title: Geniposide, an iridoid glucoside derived from Gardenia jasminoides, protects against lipopolysaccharide-induced acute lung injury in mice.
abstract: Geniposide, a main iridoid glucoside component of gardenia fruit, has been shown to possess anti-inflammatory activity. However, its potential use for acute lung injury (ALI) has not yet been studied. The aim of this study was to evaluate the anti-inflammatory properties of geniposide using a mouse ALI model. ALI was induced by intranasal injection of lipopolysaccharide (LPS). Pretreatment of mice with geniposide (20, 40, or 80 mg/kg) resulted in a marked reduction in inflammatory cells and total protein concentration in the bronchoalveolar lavage fluid (BALF) of mice. Levels of inflammatory mediators, including tumour necrosis factor- α (TNF- α), interleukin-6 (IL-6), and interleukin-10 (IL-10), were significantly altered after treatment with geniposide. Histological studies using hematoxylin and eosin (H&E) staining demonstrate that geniposide substantially inhibited LPS-induced alveolar wall changes, alveolar haemorrhage, and neutrophil infiltration in lung tissue, with evidence of reduced myeloperoxidase (MPO) activity. In addition, we investigated potential signal transduction mechanisms that could be implicated in geniposide activity. Our results suggest that geniposide may provide protective effects against LPS-induced ALI by mitigating inflammatory responses and that the compound's mechanism of action may involve blocking nuclear factor-kappaB (NF- κB) and mitogen-activated protein kinases (MAPK) signalling pathway activation.
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, College of Animal Science and Veterinary Medicine, Jilin University, Changchun, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Xiaofeng
authors.forename: Cai
authors.initials: C
authors.lastname: Qinren
authors.forename: He
authors.initials: H
authors.lastname: Jingping
authors.forename: Chu
authors.initials: C
authors.lastname: Xiao
authors.forename: Wei
authors.initials: W
authors.lastname: Miaomiao
authors.forename: Feng
authors.initials: F
authors.lastname: Xiangru
authors.forename: Xie
authors.initials: X
authors.lastname: Xianxing
authors.forename: Huo
authors.initials: H
authors.lastname: Meixia
authors.forename: Liu
authors.initials: L
authors.lastname: Jing
authors.forename: Wei
authors.initials: W
authors.lastname: Jingyuan
authors.forename: Ci
authors.initials: C
authors.lastname: Xinxin
authors.forename: Li
authors.initials: L
authors.lastname: Hongyu
authors.forename: Deng
authors.initials: D
authors.lastname: Yanhong
authors.forename: Jiang
authors.initials: J
authors.lastname: Lanxiang
authors.forename: Deng
authors.initials: D
authors.lastname: Xuming
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000517:prevention & control*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D016207:Cytokines / Q000032:analysis; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005638:Fruit / Q000737:chemistry; D032062:Gardenia / Q000737:chemistry*; D057888:Iridoid Glucosides / Q000494:pharmacology / Q000627:therapeutic use; D039823:Iridoids / Q000494:pharmacology / Q000627:therapeutic use*; D008070:Lipopolysaccharides / Q000633:toxicity; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D020929:Mitogen-Activated Protein Kinase Kinases / Q000187:drug effects / Q000378:metabolism; D016328:NF-kappa B / Q000187:drug effects / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry; D011897:Random Allocation; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D057888:Iridoid Glucosides; D039823:Iridoids; D008070:Lipopolysaccharides; D016328:NF-kappa B; C007835:geniposide; D009195:Peroxidase; D020929:Mitogen-Activated Protein Kinase Kinases