title: Real-time multiplex PCR for detecting Shiga toxin 2-producing Escherichia coli O104:H4 in human stools.
abstract: A real-time multiplex PCR targeting stx(2), wzy(O104), and fliC(H4) of enterohemorrhagic Escherichia coli (EHEC) O104:H4 correctly determined the presence or absence of these genes in 253 EHEC isolates and enrichment cultures of stool samples from 132 patients. It is a rapid, sensitive, and specific tool for detecting EHEC O104:H4 in human stools.
authors.affiliation: Institute for Hygiene and the National Consulting Laboratory for Hemolytic Uremic Syndrome, University of Münster, Münster, Germany.
authors.forename: Wenlan
authors.initials: W
authors.lastname: Zhang
authors.forename: Martina
authors.initials: M
authors.lastname: Bielaszewska
authors.forename: Andreas
authors.initials: A
authors.lastname: Bauwens
authors.forename: Angelika
authors.initials: A
authors.lastname: Fruth
authors.forename: Alexander
authors.initials: A
authors.lastname: Mellmann
authors.forename: Helge
authors.initials: H
authors.lastname: Karch
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D054324:Enterohemorrhagic Escherichia coli / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000382:microbiology*; D005243:Feces / Q000382:microbiology*; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D022641:Shiga Toxin 2 / Q000235:genetics*; D013997:Time Factors
chemical_list: D022641:Shiga Toxin 2