title: Interferon stimulated genes, CXCR4 and immune cell responses in peripheral blood mononuclear cells infected with bovine viral diarrhea virus.
abstract: Non-cytopathic bovine viral diarrhea virus (ncpBVDV) induces immune responses mediated by chemokines and interferon (IFN) stimulated genes (ISGs). Cultured bovine peripheral blood mononuclear cells (PBMC) from ncpBVDV-naïve cattle were used herein to demonstrate that BVDV infection modulates chemokine receptor 4 (CXCR4), CXCL12, IFN-I, ISGs and selected immune cell marker (CD4, CD8, CD14) mRNAs, and that these acute responses to viral infection are reflected in PBMC cultured with serum from heifers carrying fetuses persistently infected (PI) with ncpBVDV. Infection of PBMC with ncpBVDV increased IFN-β, ISG15, RIG-I, CXCR4, CXCL12, and CD8 mRNA concentrations after 32 h. Culture of PBMC with uterine vein serum from acutely infected heifers, inoculated with ncpBVDV during early gestation to generate PI fetuses, also increased the concentration of CXCR4, RIG-I and ISG15 mRNAs. In vitro PBMC treatment with ncpBVDV or uterine vein serum from acutely infected pregnant heifers activates chemokine, ISG and immune cell responses.
authors.affiliation: Animal Reproduction and Biotechnology Laboratory, Department of Biomedical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.
authors.forename: C M
authors.initials: CM
authors.lastname: Weiner
authors.forename: N P
authors.initials: NP
authors.lastname: Smirnova
authors.forename: B T
authors.initials: BT
authors.lastname: Webb
authors.forename: H
authors.initials: H
authors.lastname: Van Campen
authors.forename: T R
authors.initials: TR
authors.lastname: Hansen
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000276:immunology* / Q000635:transmission / Q000821:virology; D002417:Cattle; D001908:Diarrhea Viruses, Bovine Viral; D005260:Female; D005786:Gene Expression Regulation / Q000276:immunology; D007113:Immunity, Innate; D018445:Infectious Disease Transmission, Vertical; D007372:Interferons / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000821:virology*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase; D019718:Receptors, CXCR4 / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors; D014452:Ubiquitins / Q000235:genetics / Q000378:metabolism; D014766:Viremia
chemical_list: D015703:Antigens, CD; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D019718:Receptors, CXCR4; D014452:Ubiquitins; D007372:Interferons; D012194:RNA-Directed DNA Polymerase