title: Comparison of cartilage histopathology assessment systems on human knee joints at all stages of osteoarthritis development.
abstract: OBJECTIVE
  To compare the MANKIN and OARSI cartilage histopathology assessment systems using human articular cartilage from a large number of donors across the adult age spectrum representing all levels of cartilage degradation.
  
  
  METHODS
  Human knees (n=125 from 65 donors; age range 23-92) were obtained from tissue banks. All cartilage surfaces were macroscopically graded. Osteochondral slabs representing the entire central regions of both femoral condyles, tibial plateaus, and the patella were processed for histology and Safranin O - Fast Green staining. Slides representing normal, aged, and osteoarthritis (OA) tissue were scanned and electronic images were scored online by five observers. Statistical analysis was performed for inter- and intra-observer variability, reproducibility and reliability.
  
  
  RESULTS
  The inter-observer variability among five observers for the MANKIN system showed a similar good Intra-class correlation coefficient (ICC>0.81) as for the OARSI system (ICC>0.78). Repeat scoring by three of the five readers showed very good agreement (ICC>0.94). Both systems showed a high reproducibility among four of the five readers as indicated by the Spearman's rho value. For the MANKIN system, the surface represented by lesion depth was the parameter where all readers showed an excellent agreement. Other parameters such as cellularity, Safranin O staining intensity and tidemark had greater inter-reader disagreement.
  
  
  CONCLUSIONS
  Both scoring systems were reliable but appeared too complex and time consuming for assessment of lesion severity, the major parameter determined in standardized scoring systems. To rapidly and reproducibly assess severity of cartilage degradation, we propose to develop a simplified system for lesion volume.
authors.affiliation: Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA.
authors.forename: C
authors.initials: C
authors.lastname: Pauli
authors.forename: R
authors.initials: R
authors.lastname: Whiteside
authors.forename: F L
authors.initials: FL
authors.lastname: Heras
authors.forename: D
authors.initials: D
authors.lastname: Nesic
authors.forename: J
authors.initials: J
authors.lastname: Koziol
authors.forename: S P
authors.initials: SP
authors.lastname: Grogan
authors.forename: J
authors.initials: J
authors.lastname: Matyas
authors.forename: K P H
authors.initials: KP
authors.lastname: Pritzker
authors.forename: D D
authors.initials: DD
authors.lastname: D'Lima
authors.forename: M K
authors.initials: MK
authors.lastname: Lotz
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002358:Cartilage, Articular / Q000473:pathology*; D002983:Clinical Competence; D005260:Female; D005269:Femur / Q000473:pathology; D006801:Humans; D007719:Knee Joint / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D015588:Observer Variation; D020370:Osteoarthritis, Knee / Q000473:pathology*; D010329:Patella / Q000473:pathology; D015203:Reproducibility of Results; D012720:Severity of Illness Index; D013977:Tibia / Q000473:pathology; D055815:Young Adult