title: Nocardioides szechwanensis sp. nov. and Nocardioides psychrotolerans sp. nov., isolated from a glacier.
abstract: Two Gram-positive, rod-shaped, non-spore-forming bacteria (strains RHLT(1)-17(T) and RHLT(2)-1(T)) were isolated from Hailuogou glacier in Szechwan province, PR China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strains belonged to the genus Nocardioides and shared 97.8 % sequence similarity with each other and 97.6 and 98.4 % sequence similarity, respectively, with Nocardioides kribbensis KSL-2(T). Strain RHLT(1)-17(T) grew at 0-35 °C and strain RHLT(2)-1(T) grew at 0-25 °C. The major cellular fatty acids of strain RHLT(1)-17(T) were C(17 : 1)ω8c (32.69 %) and iso-C(16 : 0) (21.74 %). The major cellular fatty acids of strain RHLT(2)-1(T) were C(18 : 1)ω9c (28.72 %), summed feature 3 (17.14 %; comprising C(16 : 1)ω7c and/or C(16 : 1)ω6c), iso-C(16 : 0) (14.35 %), C(16 : 0) (9.96 %) and iso-C(14 : 0) (8.34 %). Both strains contained ll-2,6-diaminopimelic acid in the cell-wall peptidoglycan and MK-8(H(4)) as the predominant menaquinone. On the basis of data obtained using a polyphasic approach, two novel species, Nocardioides szechwanensis sp. nov. (type strain RHLT(1)-17(T) = CGMCC 1.11147(T) = NBRC 108562(T)) and Nocardioides psychrotolerans sp. nov. (type strain RHLT(2)-1(T) =CGMCC 1.11156(T) = NBRC 108563(T)), are proposed.
authors.affiliation: State Key Laboratories for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.affiliation: CGMCC - China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Yu-Hua
authors.initials: YH
authors.lastname: Xin
authors.affiliation: CGMCC - China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Hong-Can
authors.initials: HC
authors.lastname: Liu
authors.affiliation: CGMCC - China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Yu-Guang
authors.initials: YG
authors.lastname: Zhou
authors.affiliation: State Key Laboratories for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wen
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D046448:Ice Cover / Q000382:microbiology*; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C024223:vitamin MK 8