title: [Proapoptotic effect of angiotensin II on renal tubular epithelial cells and protective effect of Cordyceps sinensis].
abstract: OBJECTIVE
  To investigate the mechanism of the protective effect of Cordyceps sinensis (C. sinensis) on the apoptosis of cultured NRK-52E induced by angiotension II (AngII).
  
  
  METHODS
  NRK-52E cells were incubated with C. sinensis (0, 5, 10, 20, and 40 mg/L) and 10(-8) mol/ L AngII for 24, 48, 72 h. The optimal concentration of C. sinensis was selected. Either NRK-52E cells were incubated with different doses of AngII (0, 10(-12), 10(-10), 10(-8), and 10(-6) mol/L) for 24 h, or with 10(-8) mol/L AngII for 24, 48, and 72 h, to observe the effect of AngII on the apoptosis of NRK- 52E cells. The optimal concentration and time of AngII were selected. In another experiment cells were divided into 5 groups: a control, AngII (10(-8) mol/L), AngII (10(-8) mol/L)+ C. sinensis (40 mg/ L), Ang II (10(-8) mol/L)+ fosinopril (10(-5) mmol/L), and Ang II (10(-8) mol/L)+ fosinopril (10(-5) mol/ L)+C. sinensis (40 mg/L). MTT assay was used to test the changes in the proliferation of NRK-52E cultured with different concentration of C. sinensis for 24, 48, 72 h. The Annecxin V-FITC and PI stainings were applied to detect the apoptosis rate induced by AngII by flow cytometer (FCM) and to determine the eddects of C. sinensis. The activity of caspase-3 was assayed by spectrophotometry.
  
  
  RESULTS
  Certain concentrations of C. sinensis (10-40 mg/L) promoted the proliferation of NRK- 52E cells inhibited by AngII(P<0.05). AngII induced the apoptosis of NRK-52E in a dose and timedependent manner, accompanied with increased activity of caspase-3 (P<0.05). C. sinensis partially suppressed the apoptosis of NRK-52E induced by AngII, and declined the activity of caspase-3 (P<0.05). No significant difference was shown as between the fosinopril group and the fosinopril+C. sinensis group (P>0.05).
  
  
  CONCLUSIONS
  C. sinensis can suppress the apoptosis of NRK-52E by AngII, and the protective effect of C. sinensis may be inhibiting the activation of caspase-3 during the AngII-induced apoptosis of NRK-52E.
authors.affiliation: Department of Nephrology, Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Tu
authors.forename: Qiaoling
authors.initials: Q
authors.lastname: Zhou
authors.forename: Rong
authors.initials: R
authors.lastname: Tang
authors.forename: Tianfeng
authors.initials: T
authors.lastname: Tang
authors.forename: Sai
authors.initials: S
authors.lastname: Hu
authors.forename: Xiang
authors.initials: X
authors.lastname: Ao
mesh_terms: D000804:Angiotensin II / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D002460:Cell Line; D002478:Cells, Cultured; D032661:Cordyceps / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004847:Epithelial Cells / Q000166:cytology*; D006801:Humans; D007684:Kidney Tubules / Q000166:cytology*; D020011:Protective Agents / Q000494:pharmacology
chemical_list: D004365:Drugs, Chinese Herbal; D020011:Protective Agents; D000804:Angiotensin II; C505407:CASP3 protein, human; D053148:Caspase 3