title: Using linkage-specific monoclonal antibodies to analyze cellular ubiquitylation.
abstract: Antibodies that specifically recognize polyubiquitin chains containing ubiquitins linked at a particular lysine residue are powerful tools for interrogating endogenous protein modifications. Here, we describe protocols for revealing K11-, K48-, and K63-linked polyubiquitin chains by western blotting, immunoprecipitation, or immunostaining.
authors.affiliation: Physiological Chemistry Department, Genentech, Inc., South San Francisco, CA, USA.
authors.forename: Kim
authors.initials: K
authors.lastname: Newton
authors.forename: Marissa L
authors.initials: ML
authors.lastname: Matsumoto
authors.forename: Ronald E
authors.initials: RE
authors.lastname: Ferrando
authors.forename: Katherine E
authors.initials: KE
authors.lastname: Wickliffe
authors.forename: Michael
authors.initials: M
authors.lastname: Rape
authors.forename: Robert F
authors.initials: RF
authors.lastname: Kelley
authors.forename: Vishva M
authors.initials: VM
authors.lastname: Dixit
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D015153:Blotting, Western / Q000379:methods; D047468:Immunoprecipitation / Q000379:methods; D025821:Polyubiquitin / Q000737:chemistry* / Q000276:immunology* / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D044764:Ubiquitin-Activating Enzymes; D044763:Ubiquitin-Conjugating Enzymes; D044767:Ubiquitin-Protein Ligases; D054875:Ubiquitination
chemical_list: D000911:Antibodies, Monoclonal; D025821:Polyubiquitin; D044763:Ubiquitin-Conjugating Enzymes; D044767:Ubiquitin-Protein Ligases; D044764:Ubiquitin-Activating Enzymes