title: [Role of caveolin-1 on membrane estrogen receptor mediated proliferation of endothelial progenitor cells].
abstract: OBJECTIVE
  To investigate the potential role of caveolin-1 (CAV-1) on membrane estrogen receptor (mER) mediated proliferation of endothelial progenitor cells (EPCs).
  
  
  METHODS
  Bone marrow (BM)-derived EPCs were cultured. The proliferation of EPCs induced by estradiol (E₂)-BSA in the absence or presence of ICI 182, 780 (a pure ER inhibitor), MβCD and CAV-1 siRNA was determined by [³H]-thymidine incorporation. The expression of CAV-1 was detected by Western blot.
  
  
  RESULTS
  Proliferation of EPC peaked after 10(-8) mol/L E₂-BSA culture for 24 h (87.5% increase vs. control), and this effect could be inhibited by estrogen receptor blocker ICI 182, 780, indicating that mER-initiated membrane signaling pathways was involved in the proliferation effect of estrogen on EPC. Both cholesterol depletion and CAV-1 siRNA significantly attenuated E₂-BSA induced [³H]-thymidine incorporation. Western blot result confirmed that cholesterol depletion or CAV-1 siRNA significantly decreased CAV-1 protein expression (-18.6% or -41.2% vs. 10(-8) mol/L E₂-BSA alone).
  
  
  CONCLUSIONS
  Our results suggested that estradiol promoted EPC proliferation through activating CAV-1 pathway.
authors.affiliation: Medical Laboratory of Beijing University Shenzhen Hospital, Shenzhen 518036, China.
authors.forename: Fei-xue
authors.initials: FX
authors.lastname: Hu
authors.forename: Ting-huai
authors.initials: TH
authors.lastname: Wang
authors.forename: Zhi
authors.initials: Z
authors.lastname: Tan
mesh_terms: D000818:Animals; D051242:Caveolin 1 / Q000276:immunology / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011960:Receptors, Estrogen / Q000378:metabolism*; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: D051242:Caveolin 1; D011960:Receptors, Estrogen; D004958:Estradiol