title: Tumour necrosis factor-alpha stimulates liver regeneration in porcine model of partial portal vein ligation.
abstract: OBJECTIVE
  Portal vein ligation (PVL) could multiply the future liver remnant volume (FLRV). Tumor necrosis factor- alpha (TNF-α) is a pleiotropic cytokine that is connected with initial phase of liver regeneration. The aim of this basic pilot study was to accelerate regeneration of liver parenchyma after PVL. The experimental porcine model was developed to be as much compatible as possible with portal vein embolization (PVE) in human medicine.
  
  
  METHODS
  After ligation of portal branches of caudate and right lateral and right medial liver lobes recombinant porcine TNF-α (TNF-α group) or physiological solution (control group) were applied into non-occluded portal vein branches. The biochemical and immunoanalytical parameters were assessed. The compensatory hypertrophy was evaluated by periodic ultrasonography. The histological examination of liver was performed.
  
  
  RESULTS
  The acceleration of growth of hypertrophic liver lobes was maximal at the 7th postoperative day in comparison with the control group (p<0.05); nevertheless this stimulating effect was lost at the end of experiment. The important differences in biochemical or histological studied parametres between study groups were not proved.
  
  
  CONCLUSIONS
  The achieved acceleration of growth of hypertrophic liver lobes after application of TNF-α confirms the role of studied cytokine in priming of liver regeneration.
authors.affiliation: Department of Surgery, Charles University, Prague, Czech Republic. vena.liska@seznam.cz
authors.forename: Vaclav
authors.initials: V
authors.lastname: Liska
authors.forename: Vladislav
authors.initials: V
authors.lastname: Treska
authors.forename: Hynek
authors.initials: H
authors.lastname: Mirka
authors.forename: Jiri
authors.initials: J
authors.lastname: Kobr
authors.forename: Roman
authors.initials: R
authors.lastname: Sykora
authors.forename: Tomas
authors.initials: T
authors.lastname: Skalicky
authors.forename: Alan
authors.initials: A
authors.lastname: Sutnar
authors.forename: Jan
authors.initials: J
authors.lastname: Bruha
authors.forename: Ondrej
authors.initials: O
authors.lastname: Fiala
authors.forename: Ondrej
authors.initials: O
authors.lastname: Vycital
authors.forename: Alena
authors.initials: A
authors.lastname: Chlumska
authors.forename: Lubos
authors.initials: L
authors.lastname: Holubec
authors.forename: Stanislav
authors.initials: S
authors.lastname: Kormunda
authors.forename: Ladislav
authors.initials: L
authors.lastname: Trefil
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Racek
authors.forename: Martin
authors.initials: M
authors.lastname: Matejovic
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015415:Biomarkers / Q000097:blood; D049109:Cell Proliferation / Q000187:drug effects; D004195:Disease Models, Animal; D022781:Hepatocytes / Q000187:drug effects* / Q000473:pathology; D008026:Ligation; D008099:Liver / Q000098:blood supply* / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008115:Liver Regeneration / Q000187:drug effects*; D011169:Portal Vein / Q000601:surgery*; D011994:Recombinant Proteins / Q000494:pharmacology; D013552:Swine; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000494:pharmacology*; D014463:Ultrasonography
chemical_list: D015415:Biomarkers; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha