title: Micellar electrokinetic chromatography-chemiluminescent detection of biogenic amines using N-(4-aminobutyl)-N-ethylisoluminol as derivatization reagent and trivalent copper chelate as chemiluminescence enhancer.
abstract: A facile, sensitive and universal method was established for analysis of biogenic amines using micellar electrokinetic chromatography coupled with chemiluminescent (CL) detection. It was found that diperiodatocuprate (III) (K(5)[Cu(HIO(6))(2)], DPC), a transition metal chelate at unstable high oxidation state, could effectively enhance the reaction between luminol-type compound and hydrogen peroxide, to produce very strong CL signal. In addition, triethylamine was found to be able to effectively improve the yield of the derivatization reaction between biogenic amines and a luminol-type derivatization reagent, N-(4-aminobutyl)-N-ethylisoluminol (ABEI). Based on these facts, three biogenic amines were pre-column derivatized with ABEI, and post-column detected using high sensitive luminol-hydrogen peroxide-DPC CL system. Since the background was quite low, and the signal was quite strong, a considerable improved sensitivity was obtained. The presented method had been successfully applied to simultaneously analyze glycine, proline and phenylalanine with the detection limits (S/N=3) of 0.030 μmol L(-1), 0.23 μmol L(-1) and 0.21 μmol L(-1), respectively. To evaluate its potential application value, glycine in saliva and urine samples was detected using this method, and satisfied results were obtained. This approach can be further extended to detection of many other compounds such as peptides and drugs by using luminol-type derivatization reagent.
authors.affiliation: Key Laboratory on Luminescence and Real-Time Analysis (Ministry of Education), College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.forename: Haoyue
authors.initials: H
authors.lastname: Xie
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Fu
mesh_terms: D000596:Amino Acids / Q000032:analysis* / Q000652:urine; D001679:Biogenic Amines / Q000032:analysis* / Q000652:urine; D002614:Chelating Agents / Q000737:chemistry*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D003300:Copper / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000379:methods*; D008165:Luminol / Q000031:analogs & derivatives* / Q000737:chemistry; D012463:Saliva / Q000737:chemistry
chemical_list: D000596:Amino Acids; D001679:Biogenic Amines; D002614:Chelating Agents; C561085:N-(4-aminobutyl)-N-ethylisoluminol; D008165:Luminol; D003300:Copper