title: Molecular identification of a Trichinella isolate from a naturally infected pig in Tibet, China.
abstract: The first human case with trichinellosis was reported in 1964 in Tibet, China. However, up to the present, the etiological agent of trichinellosis has been unclear. The aim of this study was to identify a Tibet Trichinella isolate at a species level by PCR-based methods. Multiplex PCR revealed amplicon of the expected size (173 bp) for Trichinella spiralis in assays containing larval DNA from Tibet Trichinella isolate from a naturally infected pig. The Tibet Trichinella isolate was also identified by PCR amplification of the 5S ribosomal DNA intergenic spacer region (5S ISR) and mitochondrial large-subunit ribosomal RNA (mt-lsrDNA) gene sequences. The results showed that 2 DNA fragments (749 bp and 445 bp) of the Tibet Trichinella isolate were identical to that of the reference isolates of T. spiralis. The Tibet Trichinella isolate might be classifiable to T. spiralis. This is the first report on T. spiralis in southwestern China.
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou 450052, Henan Province, PR China.
authors.forename: Ling Zhao
authors.initials: LZ
authors.lastname: Li
authors.forename: Zhong Quan
authors.initials: ZQ
authors.lastname: Wang
authors.forename: Peng
authors.initials: P
authors.lastname: Jiang
authors.forename: Xi
authors.initials: X
authors.lastname: Zhang
authors.forename: Hui Jun
authors.initials: HJ
authors.lastname: Ren
authors.forename: Jing
authors.initials: J
authors.lastname: Cui
mesh_terms: D000818:Animals; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005838:Genotype; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction; D012341:RNA, Ribosomal, 5S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013552:Swine; D013553:Swine Diseases / Q000469:parasitology*; D018609:Tibet; D017160:Trichinella spiralis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014235:Trichinellosis / Q000469:parasitology / Q000662:veterinary*
chemical_list: D018509:DNA, Helminth; D004272:DNA, Mitochondrial; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D012341:RNA, Ribosomal, 5S