title: Harvesting of Chlorella sp. using hollow fiber ultrafiltration.
abstract: BACKGROUND
  The suitability of the application of ultrafiltration (UF) to harvest Chlorella sp. from the culture medium was examined. We investigated the effects of two improved UF system, forward air-water flushing and backwash with permeate, on the concentration process.
  
  
  METHODS
  Backwash with permeate was selected as an optimization of the improved UF system, which was more effective for permeate flux recovery. Moreover, the hollow fiber UF system by adding periodical backwash with permeate was examined for Chlorella sp. harvesting.
  
  
  CONCLUSIONS
  It was found that Chlorella sp. could be concentrated with high recovery in a lab-scale experiment. An overall algal biomass recovery of above 90% was achieved when the volume concentration factor was 10. For an original biomass of 1.3 ± 0.05 g/L, 1 min backwash followed by 20 min forward concentrating was more effective, which resulted in a recovery of 94% and a high average flux of 30.3 L/m(2)/h. In addition, the algal recovery was highly correlated to the volume concentration factor and the initial biomass. A high concentration factor or a high initial biomass resulted in a low biomass recovery.
authors.affiliation: Key Laboratory of Biofuels, Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, People's Republic of China.
authors.forename: Cui
authors.initials: C
authors.lastname: Huang
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Chen
authors.forename: Tianzhong
authors.initials: T
authors.lastname: Liu
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Yang
authors.forename: Yong
authors.initials: Y
authors.lastname: Xiao
authors.forename: Guangming
authors.initials: G
authors.lastname: Zeng
authors.forename: Xiuxuan
authors.initials: X
authors.lastname: Sun
mesh_terms: D018533:Biomass; D002708:Chlorella; D003470:Culture Media; D007218:Industrial Microbiology / Q000379:methods; D014462:Ultrafiltration / Q000295:instrumentation / Q000379:methods*
chemical_list: D003470:Culture Media