title: [Preclinical studies of live intranasal H5N1 influenza vaccine with the deleted HS1 gene].
abstract: The paper gives the results of evaluating the efficiency of deINS1 pandemic H5N1 vaccine candidate VN1203delNS1 which was constructed by reverse genetics on the basis of influenza virus strain A/Vietnam/1203/04. The safety, immunogenicity and cross-protection of the vaccine strain against different H5N1 virus clades were demonstrated in mouse and macaque models. The results showed the possibility of designing a new-generation replication-deficient intranasal influenza vaccine, by applying an approach to deleting the NS1 pathogenicity factor, an antagonist of the interferon system.
authors.forename: E A
authors.initials: EA
authors.lastname: Romanovskaia-Roman'ko
authors.forename: B
authors.initials: B
authors.lastname: Ferko
authors.forename: O I
authors.initials: OI
authors.lastname: Vyshemirskiĭ
authors.forename: Iu R
authors.initials: IuR
authors.lastname: Romanova
authors.forename: B
authors.initials: B
authors.lastname: Krenn
authors.forename: T
authors.initials: T
authors.lastname: Muster
authors.forename: M P
authors.initials: MP
authors.lastname: Grudinin
authors.forename: B A
authors.initials: BA
authors.lastname: Lapin
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Egorov
authors.forename: O I
authors.initials: OI
authors.lastname: Kiselev
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D002522:Chlorocebus aethiops; D056738:Cross Protection / Q000276:immunology; D004353:Drug Evaluation, Preclinical; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics* / Q000276:immunology; D007252:Influenza Vaccines / Q000235:genetics / Q000276:immunology / Q000627:therapeutic use*; D007251:Influenza, Human / Q000235:genetics / Q000276:immunology / Q000517:prevention & control*; D007372:Interferons / Q000378:metabolism; D008252:Macaca fascicularis; D051379:Mice; D009976:Orthomyxoviridae Infections / Q000235:genetics / Q000276:immunology / Q000517:prevention & control*; D059386:Reverse Genetics / Q000379:methods; D014613:Vaccines, Attenuated / Q000235:genetics / Q000276:immunology / Q000627:therapeutic use*; D014709:Vero Cells; D017361:Viral Nonstructural Proteins / Q000235:genetics* / Q000276:immunology
chemical_list: D007252:Influenza Vaccines; D014613:Vaccines, Attenuated; D017361:Viral Nonstructural Proteins; D007372:Interferons