title: Highly sensitive DNA hybridization detection with single nanoparticle flash-lamp darkfield microscopy.
abstract: We developed a novel method for counting single gold nanoparticles in free solution with flash-lamp darkfield microscopy and demonstrated that it could be applied to highly sensitive DNA hybridization detection based on target DNA induced AuNP aggregation and counts reduction.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, P. R. China.
authors.forename: Zhiqin
authors.initials: Z
authors.lastname: Yuan
authors.forename: Jing
authors.initials: J
authors.lastname: Cheng
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Cheng
authors.forename: Yan
authors.initials: Y
authors.lastname: He
authors.forename: Edward S
authors.initials: ES
authors.lastname: Yeung
mesh_terms: D004247:DNA / Q000737:chemistry*; D057230:Limit of Detection; D008853:Microscopy / Q000379:methods*; D053758:Nanoparticles; D009693:Nucleic Acid Hybridization
chemical_list: D004247:DNA