title: A simple freezing medium for serum-free cultured cells.
abstract: Methylcellulose was found to protect serum-free cultured cells from the deleterious effects of freezing and thawing. We have formulated a simple medium suitable for freezing serum-free cultured cells that consists of 0.1% methylcellulose, 10% dimethylsulfoxide, and MEM or any other serum-free culture medium.
authors.affiliation: GENE BANK, The Institute of Physical and Chemical Research, (RIKEN), 3-1-1 Koyadai, 305, Tsukuba-shi, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ohno
authors.forename: K
authors.initials: K
authors.lastname: Kurita
authors.forename: S
authors.initials: S
authors.lastname: Abe
authors.forename: N
authors.initials: N
authors.lastname: Eimori
authors.forename: Y
authors.initials: Y
authors.lastname: Ikawa