title: Measurement of pentosidine in biological samples.
abstract: Pentosidine is a highly fluorescent advanced glycation end product (AGE) and crosslink derived from one molecule of arginine and one of lysine bridged in an imidazo-pyridinium structure (Fig. 1). It was first isolated from articular cartilage by Sell and Monnier (1), and has now been detected and quantified in a variety of human and animal tissues, including skin and kidney collagen (2-5), lens crystallins (6, 7), plasma (8, 9), serum (10), urine (11), and synovial fluid (12, 13). Pentosidine is readily prepared from arginine, lysine, and a pentose (hence its name). Dyer et al. (14) have also described its formation from glucose, albeit at a slower rate and probably through oxidation of glucose to arabinose (15). Because its formation from either glucose or ribose requires oxidation, pentosidine is both an AGE and a "glycoxidation" product (16).                                         Fig. 1.                                            Structure of pentosidine.
authors.affiliation: Department of Chemistry and Biochemistry, University of Southern Carolina, Columbia, SC.
authors.forename: J R
authors.initials: JR
authors.lastname: Requena
authors.forename: D L
authors.initials: DL
authors.lastname: Price
authors.forename: S R
authors.initials: SR
authors.lastname: Thorpe
authors.forename: J W
authors.initials: JW
authors.lastname: Baynes