title: Prevention of apoptosis by mitochondrial phosphatase PGAM5 in the mushroom body is crucial for heat shock resistance in Drosophila melanogaster.
abstract: The heat shock (HS) response is essential for survival of all organisms. Although the machinery of the HS response has been extensively investigated at the cellular level, it is poorly understood at the level of the organism. Here, we show the crucial role of the mushroom body (MB) in the HS response in Drosophila. Null mutants of the mitochondrial phosphatase Drosophila PGAM5 (dPGAM5) exhibited increased vulnerability to HS, which was reversed by MB-specific expression of the caspase inhibitor p35, and similar vulnerability was induced in wild-type flies by knockdown of MB dPGAM5. Elimination of the MB did not affect the HS response of wild-type flies, but did increase the resistance of dPGAM5-deficient flies to HS. Thus, the MB may possess an apoptosis-dependent toxic function, the suppression of which by dPGAM5 appears to be crucial for HS resistance.
authors.affiliation: Laboratory of Cell Signaling, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan.
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Ishida
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Sekine
authors.forename: Haruka
authors.initials: H
authors.lastname: Oguchi
authors.forename: Takahiro
authors.initials: T
authors.lastname: Chihara
authors.forename: Masayuki
authors.initials: M
authors.lastname: Miura
authors.forename: Hidenori
authors.initials: H
authors.lastname: Ichijo
authors.forename: Kohsuke
authors.initials: K
authors.lastname: Takeda
mesh_terms: D000818:Animals; D017209:Apoptosis; D029721:Drosophila Proteins / Q000502:physiology*; D004331:Drosophila melanogaster / Q000166:cytology / Q000201:enzymology*; D018869:Heat-Shock Response; D024101:Mitochondrial Proteins; D024521:Mushroom Bodies / Q000737:chemistry*; D010749:Phosphoprotein Phosphatases / Q000502:physiology*
chemical_list: D029721:Drosophila Proteins; D024101:Mitochondrial Proteins; C573812:PGAM5 protein, Drosophila; D010749:Phosphoprotein Phosphatases