title: Specific Y14 domains mediate its nucleo-cytoplasmic shuttling and association with spliced mRNA.
abstract: Pre-mRNA splicing deposits multi-protein complexes, termed exon junction complexes (EJCs), on mRNAs near exon-exon junctions. The core of EJC consists of four proteins, eIF4AIII, MLN51, Y14 and Magoh. Y14 is a nuclear protein that can shuttle between the nucleus and the cytoplasm, and binds specifically to Magoh. Here we delineate a Y14 nuclear localization signal that also confers its nuclear export, which we name YNS. We further identified a 12-amino-acid peptide near Y14's carboxyl terminus that is required for its association with spliced mRNAs, as well as for Magoh binding. Furthermore, the Y14 mutants, which are deficient in binding to Magoh, could still be localized to the nucleus, suggesting the existence of both the nuclear import pathway and function for Y14 unaccompanied by Magoh.
authors.affiliation: Medical Top Track Program, Medical Research Institute, Tokyo Dental and Medical University, Tokyo 113-8510, Japan. kataoka.naoyuki.6m@kyoto-u.ac.jp
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Kataoka
authors.forename: Michael D
authors.initials: MD
authors.lastname: Diem
authors.forename: Mayumi
authors.initials: M
authors.lastname: Yoshida
authors.forename: Chihiro
authors.initials: C
authors.lastname: Hatai
authors.forename: Izumi
authors.initials: I
authors.lastname: Dobashi
authors.forename: Gideon
authors.initials: G
authors.lastname: Dreyfuss
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Hagiwara
authors.forename: Mutsuhito
authors.initials: M
authors.lastname: Ohno
mesh_terms: D000595:Amino Acid Sequence; D002467:Cell Nucleus / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism*; D005091:Exons; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D049790:Nuclear Export Signals; D021381:Protein Transport; D012326:RNA Splicing; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D049790:Nuclear Export Signals; C406243:RBM8A protein, human; D012333:RNA, Messenger; D016601:RNA-Binding Proteins