title: Opposing function of MYBBP1A in proliferation and migration of head and neck squamous cell carcinoma cells.
abstract: BACKGROUND
  Head and neck squamous cell carcinoma (HNSCC) is one of the most prevalent and lethal cancers worldwide and mortality mostly results from loco-regional recurrence and metastasis. Despite its significance, our knowledge on molecular, cellular and environmental mechanisms that drive disease pathogenesis remains largely elusive, and there are limited therapeutic options, with only negligible clinical benefit.
  
  
  METHODS
  We applied global gene expression profiling with samples derived from a recently established mouse model for oral cancer recurrence and identified a list of genes with differential expression between primary and recurrent tumors.
  
  
  RESULTS
  One differentially expressed gene codes for Myb-binding protein 1a (MYBBP1A), which is known as a transcriptional co-regulator that physically interacts with nuclear transcription factors, such as NFκB and p53. We confirmed significantly reduced MYBBP1A protein levels on tissue sections of recurrent mouse tumors compared to primary tumors by immunohistochemistry, and found aberrant MYBBP1A protein levels also in tumor samples of HNSCC patients. Interestingly, silencing of MYBBP1A expression in murine SCC7 and in human HNSCC cell lines elicited increased migration but decreased cell growth.
  
  
  CONCLUSIONS
  We provide experimental evidence that MYBBP1A is an important molecular switch in the regulation of tumor cell proliferation versus migration in HNSCC and it will be a major challenge for the future to proof the concept whether regulation MYBBP1A expression and/or function could serve as a novel option for anti-cancer therapy.
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, University Hospital Heidelberg, Heidelberg, Germany.
authors.forename: Gustavo A
authors.initials: GA
authors.lastname: Acuña Sanhueza
authors.forename: Leonie
authors.initials: L
authors.lastname: Faller
authors.forename: Babitha
authors.initials: B
authors.lastname: George
authors.forename: Jennifer
authors.initials: J
authors.lastname: Koffler
authors.forename: Vinko
authors.initials: V
authors.lastname: Misetic
authors.forename: Christa
authors.initials: C
authors.lastname: Flechtenmacher
authors.forename: Gerhard
authors.initials: G
authors.lastname: Dyckhoff
authors.forename: Peter P
authors.initials: PP
authors.lastname: Plinkert
authors.forename: Peter
authors.initials: P
authors.lastname: Angel
authors.forename: Christian
authors.initials: C
authors.lastname: Simon
authors.forename: Jochen
authors.initials: J
authors.lastname: Hess
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D004268:DNA-Binding Proteins; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D009062:Mouth Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D009364:Neoplasm Recurrence, Local / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D029543:Nucleocytoplasmic Transport Proteins / Q000235:genetics* / Q000378:metabolism; D016601:RNA-Binding Proteins; D060888:Real-Time Polymerase Chain Reaction; D014157:Transcription Factors
chemical_list: D004268:DNA-Binding Proteins; C491719:MYBBP1A protein, human; D009687:Nuclear Proteins; D029543:Nucleocytoplasmic Transport Proteins; D016601:RNA-Binding Proteins; D014157:Transcription Factors