title: Antibiofilm activity of α-amylase from Bacillus subtilis S8-18 against biofilm forming human bacterial pathogens.
abstract: The extracellular α-amylase enzyme from Bacillus subtilis S8-18 of marine origin was proved as an antibiofilm agent against methicillin-resistant Staphylococcus aureus (MRSA), a clinical strain isolated from pharyngitis patient, Vibrio cholerae also a clinical isolate from cholera patient and Pseudomonas aeruginosa ATCC10145. The spectrophotometric and microscopic investigations revealed the potential of α-amylase to inhibit biofilm formation in these pathogens. At its BIC level, the crude enzyme caused 51.81-73.07% of biofilm inhibition. Beyond the inhibition, the enzyme was also effective in degradation of preformed mature biofilm by disrupting the exopolysaccharide (EPS), an essential component in biofilm architecture. Furthermore, the enzyme purified to its homogeneity by chromatographic techniques was also effective in biofilm inhibition (43.83-61.68%) as well as in degradation of EPS. A commercial α-amylase enzyme from B. subtilis was also used for comparative purpose. Besides, the effect of various enzymes and temperature on the antibiofilm activity of amylase enzymes was also investigated. This study, for the first time, proved that α-amylase enzyme alone can be used to inhibit/disrupt the biofilms of V. cholerae and MRSA strains and beholds much promise in clinical applications.
authors.affiliation: Department of Biotechnology, Alagappa University, Karaikudi 630 003, Tamil Nadu, India.
authors.forename: Balu Jancy
authors.initials: BJ
authors.lastname: Kalpana
authors.forename: Subramonian
authors.initials: S
authors.lastname: Aarthy
authors.forename: Shunmugiah Karutha
authors.initials: SK
authors.lastname: Pandian
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology*; D018441:Biofilms; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects*; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D013696:Temperature; D000516:alpha-Amylases / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000516:alpha-Amylases