title: Anti-inflammatory and antinociceptive effects of cordymin, a peptide purified from the medicinal mushroom Cordyceps sinensis.
abstract: The anti-inflammatory and antinociceptive activities of cordymin, a peptide purified from the medicinal mushroom Cordyceps sinensis, were studied. The effects of cordymin on cytokine levels and total antioxidant activity were analysed. The antinociceptive effects of cordymin in vivo and in vitro were also determined. Cordymin treatment decreased the levels of tumour necrosis factor alpha, interleukin 1 beta and total antioxidant status. Cordymin inhibited the acetic acid-induced abdominal constrictions in mice in a dose-dependent manner. In the hot-plate test, results showed that cordymin significantly inhibited the reaction time to thermal stimuli at 30, 60 and 90 min. In neurolysin inhibition assay, cordymin showed strong activities against neurolysin (IC(50) = 0.1 µM). Our results show that cordymin is a potent anti-inflammatory and analgesic medicine.
authors.affiliation: Department of Traditional Chinese Medicine, Zhejiang Pharmaceutical College, Ningbo 315100, People's Republic of China.
authors.forename: Gui-min
authors.initials: GM
authors.lastname: Qian
authors.forename: Guo-Feng
authors.initials: GF
authors.lastname: Pan
authors.forename: Jian-You
authors.initials: JY
authors.lastname: Guo
mesh_terms: D019342:Acetic Acid / Q000633:toxicity; D000700:Analgesics / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D003085:Colic / Q000139:chemically induced / Q000188:drug therapy; D032661:Cordyceps / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D006358:Hot Temperature; D053583:Interleukin-1beta / Q000097:blood; D008666:Metalloendopeptidases / Q000037:antagonists & inhibitors; D051379:Mice; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D011930:Reaction Time / Q000187:drug effects; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D000700:Analgesics; D000893:Anti-Inflammatory Agents; D053583:Interleukin-1beta; D010455:Peptides; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; C572165:cordymin; D008666:Metalloendopeptidases; C062316:neurolysin; D019342:Acetic Acid