title: A 24 kDa excretory-secretory protein of Anisakis simplex larvae could elicit allergic airway inflammation in mice.
abstract: We have reported that a 24 kDa protein (22U homologous; As22U) of Anisakis simplex larvae could elicit several Th2-related chemokine gene expressions in the intestinal epithelial cell line which means that As22U may play a role as an allergen. In order to determine the contribution of As22U to allergic reactions, we treated mice with 6 times intra-nasal application of recombinant As22U (rAs22U). In the group challenged with rAs22U and ovalbumin (OVA), the number of eosinophils in the bronchial alveolar lavage fluid (BALF) was significantly increased, as compared to the group receiving only OVA. In addition, mice treated with rAs22U and OVA showed significantly increased airway hyperresponsiveness. Thus, severe inflammation around the airway and immune cell recruitment was observed in mice treated with rAs22U plus OVA. The levels of IL-4, IL-5, and IL-13 cytokines in the BALF increased significantly after treatment with rAs22U and OVA. Similarly, the levels of anti-OVA specific IgE and IgG1 increased in mice treated with rAs22U and OVA, compared to those treated only with OVA. The Gro-α (CXCL1) gene expression in mouse lung epithelial cells increased instantly after treatment with rAs22U, and allergy-specific chemokines eotaxin (CCL11) and thymus-and-activation-regulated-chemokine (CCL17) gene expressions significantly increased at 6 hr after treatment. In conclusion, rAs22U may induce airway allergic inflammation, as the result of enhanced Th2 and Th17 responses.
authors.affiliation: Department of Internal Medicine, Pusan National University, Yangsan 626-870, Korea.
authors.forename: Hye-Kyung
authors.initials: HK
authors.lastname: Park
authors.forename: Min Kyoung
authors.initials: MK
authors.lastname: Cho
authors.forename: Mi Kyung
authors.initials: MK
authors.lastname: Park
authors.forename: Shin Ae
authors.initials: SA
authors.lastname: Kang
authors.forename: Yun Seong
authors.initials: YS
authors.lastname: Kim
authors.forename: Ki Uk
authors.initials: KU
authors.lastname: Kim
authors.forename: Min Ki
authors.initials: MK
authors.lastname: Lee
authors.forename: Mee Sun
authors.initials: MS
authors.lastname: Ock
authors.forename: Hee Jae
authors.initials: HJ
authors.lastname: Cha
authors.forename: Hak Sun
authors.initials: HS
authors.lastname: Yu
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D017129:Anisakiasis / Q000276:immunology* / Q000469:parasitology; D017163:Anisakis / Q000276:immunology* / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid; D018925:Chemokines / Q000378:metabolism; D016207:Cytokines / Q000032:analysis / Q000378:metabolism*; D004804:Eosinophils / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation / Q000276:immunology*; D015801:Helminth Proteins / Q000276:immunology*; D006967:Hypersensitivity / Q000276:immunology* / Q000469:parasitology; D007073:Immunoglobulin E / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007814:Larva / Q000276:immunology / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D011994:Recombinant Proteins / Q000276:immunology; D058504:Th17 Cells / Q000378:metabolism; D018418:Th2 Cells / Q000378:metabolism
chemical_list: D018925:Chemokines; D016207:Cytokines; D015801:Helminth Proteins; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D007073:Immunoglobulin E