title: The p19 protein of Grapevine Algerian latent virus is a determinant of systemic infection of Chenopodium quinoa.
abstract: A previous study showed that both Grapevine Algerian latent virus (GALV) and Tomato bushy stunt virus (TBSV) systemically infect Nicotiana benthamiana, but GALV causes systemic infection whereas TBSV causes only local lesions in Chenopodium quinoa (C. quinoa). We recently isolated GALV strain Naju (GALV-N) from Limonium sinense and TBSV strain Sacheon (TBSV-S) from tomato. Both viruses belong to the genus Tombusvirus and have a similar genome organization. To identify determinants of systemic infection of GALV-N in C. quinoa in the current study, we generated infectious clones and capsid protein (CP)-deletion clones for the two viruses and confirmed that CP of GALV-N is required for systemic infection of C. quinoa due to its primary structural role in virus assembly. Through the use of chimeras, we identified a viral factor in addition to CP that contributes to systemic infection by GALV-N. Inactivation of the p19 demonstrated that host-specific activities of p19 are necessary for efficient systemic infection of C. quinoa by GALV-N. Our study is the first report to determine the viral factors required for systemic infection of GALV in C. quinoa.
authors.affiliation: Department of Agricultural Biotechnology and Plant Genomics and Breeding Institute, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: Semin
authors.initials: S
authors.lastname: Kim
authors.forename: Won Kyong
authors.initials: WK
authors.lastname: Cho
authors.forename: Hyeok-Geun
authors.initials: HG
authors.lastname: Lee
authors.forename: Sang-Ho
authors.initials: SH
authors.lastname: Park
authors.forename: Seong-Han
authors.initials: SH
authors.lastname: Sohn
authors.forename: Kook-Hyung
authors.initials: KH
authors.lastname: Kim
mesh_terms: D027465:Chenopodium quinoa / Q000821:virology*; D058507:Host Specificity; D010935:Plant Diseases / Q000821:virology*; D017864:Tombusvirus / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D014774:Virulence
chemical_list: D014764:Viral Proteins