title: Characterization of a new human cell line (CH-3573) derived from a grade II chondrosarcoma with matrix production.
abstract: Chondrosarcomas are malignant cartilage-forming tumors that represent the third most common malignant solid tumor of bone. In patients with grades II and III, local recurrence, increasing tumor size and dedifferentiation have been associated with lower survival rates. These biologically poorly-understood neoplasms vary considerably in clinical presentation and biological behavior. Cytogenetic studies have shown that heterogeneity is related to karyotypic complexity; moreover, alterations in the 9p21 locus and TP53 gene are related to disease progression. Despite the relatively high frequency of chondrosarcoma only a limited number of cell lines exist in the scientific community, limiting the possibility to study hypothesis-derived research or primary drug interaction necessary for pre-clinical studies. We report a chondrosarcoma cell line, CH-3573, derived from a primary tumor that may serve as a useful tool for both in vitro and in vivo models to study the molecular pathogenesis. In addition, xenograft passages in nude mice were studied to characterize the genetic stability over the course of tumor progression. In contrary to other reported cell lines, an important feature of our established cell line was the retained matrix production, a characteristic feature of a conventional grade II chondrosarcoma. The cell line (CH-3573) was characterized by pathological, immunohistochemical and molecular genetic methods.
authors.affiliation: Department of Pathology, University of Valencia, Avda. Blasco Ibañez, 17, 46010, Valencia, Spain.
authors.forename: Silvia
authors.initials: S
authors.lastname: Calabuig-Fariñas
authors.forename: Rosario Gil
authors.initials: RG
authors.lastname: Benso
authors.forename: Karoly
authors.initials: K
authors.lastname: Szuhai
authors.forename: Isidro
authors.initials: I
authors.lastname: Machado
authors.forename: José Antonio
authors.initials: JA
authors.lastname: López-Guerrero
authors.forename: Danielle
authors.initials: D
authors.lastname: de Jong
authors.forename: Amando
authors.initials: A
authors.lastname: Peydró
authors.forename: Teresa
authors.initials: T
authors.lastname: San Miguel
authors.forename: Lara
authors.initials: L
authors.lastname: Navarro
authors.forename: Antonio
authors.initials: A
authors.lastname: Pellín
authors.forename: Antonio
authors.initials: A
authors.lastname: Llombart-Bosch
mesh_terms: D000328:Adult; D000818:Animals; D001859:Bone Neoplasms / Q000737:chemistry / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D002813:Chondrosarcoma / Q000737:chemistry / Q000235:genetics / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D051379:Mice; D008819:Mice, Nude; D060787:Neoplasm Grading; D009363:Neoplasm Proteins / Q000737:chemistry / Q000378:metabolism; D014183:Transplantation, Heterologous
chemical_list: D009363:Neoplasm Proteins