title: Serotyping and Cross-Reactivity's Between Different Pseudomonas aeruginosa Isolates Prevalent in Iran.
abstract: OBJECTIVE
  300 Pseudomonas aeruginosa strains were isolated from hospitalized patients in Iran. Using international antigenic typing system (IATS) antibodies, all strains were classified into 16 serotypes while serotype 14 was not identified among the 17 known serotypes. To evaluate the rate of cross-reactivity between O- antigenic determinants, monospecific polyclonal antibodies were made against whole-killed-cells and live cells of each serotype.
  
  
  METHODS
  Each antiserum was challenged against homologous and heterologous antigens using slide agglutination test. The degree of agglutination reaction is shown by -ve, 1+ve, 2+ve, 3+ve and 4+ve for 0, 25%, 50%, 75% and 100% agglutination respectively. Then, the results were tabulated for further study.
  
  
  RESULTS
  The rate of cross-reactivity between O-antigenic determinants demonstrated that strains 10.55 and 15.14 had the highest agglutination reaction with serum of all the homologous and heterologous serotypes.
  
  
  CONCLUSIONS
  Evaluation of the results obtained from the present study can be applied in production of reliable vaccines and antisera as therapeutic agents or as diagnostic kits.
authors.affiliation: Department of Bacterial Vaccines and Antigen Production, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: H
authors.initials: H
authors.lastname: Ahmadi
authors.forename: S
authors.initials: S
authors.lastname: Maleknia
authors.forename: B
authors.initials: B
authors.lastname: Tabaraie
authors.forename: D
authors.initials: D
authors.lastname: Norouzian
authors.forename: F
authors.initials: F
authors.lastname: Poormirza-Gholi
authors.forename: M
authors.initials: M
authors.lastname: Nejati
authors.forename: Mh
authors.initials: M
authors.lastname: Hedayati
authors.forename: Mr
authors.initials: M
authors.lastname: Beik Mohammadi
authors.forename: A
authors.initials: A
authors.lastname: Behnoodi
authors.forename: M
authors.initials: M
authors.lastname: Izadpanahi