title: Validated LC-MS-MS method for the determination of quetiapine in human plasma: application to a pharmacokinetic study.
abstract: A sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS-MS) method for the determination of quetiapine was developed and validated over the linearity range 1-1500 ng/mL with 0.1 mL of plasma using clozapine as the internal standard. Detection was performed on a triple-quadrupole tandem mass spectrometer using positive electrospray ionization and quantification was performed by selected reaction monitoring mode. The MS-MS ion transitions monitored were m/z 384.1 → 253.1 and 327.0 → 270.0 for quetiapine and clozapine, respectively. The between- and within-run precision was less than 7.44% and accuracy was less than 10.2%. The lower limit of quantification was 1 ng/mL. The extraction recoveries of quetiapine were over 90%. The method is proved to be accurate and specific, and was applied to the pharmacokinetic study in healthy Chinese volunteers.
authors.affiliation: Department of Disaster Management, Taiwan Police College, Taipei, Taiwan, Republic of China.
authors.forename: Ryh-Nan
authors.initials: RN
authors.lastname: Pan
authors.forename: Benjamin Pei-Chung
authors.initials: BP
authors.lastname: Kuo
authors.forename: Li-Heng
authors.initials: LH
authors.lastname: Pao
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D003024:Clozapine / Q000097:blood; D003987:Dibenzothiazepines / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D004355:Drug Stability; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D059625:Liquid-Liquid Extraction; D008297:Male; D000069348:Quetiapine Fumarate; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D003987:Dibenzothiazepines; D000069348:Quetiapine Fumarate; D003024:Clozapine