title: Production of human-mouse chimeric antibody by high cell density perfusion culture.
abstract: Two mouse myeloma cell lines which were transfected with chimeric mouse variable-human constant immunoglobulin heavy and light chain genes have been cultured at high cell density in a settling perfusion culture vessel to produce chimeric antibody specific for human common acute lymphocytic leukemia antigen (cALLA).J558L transfectant proliferated well in a serum-free medium (ITES-eRDF) to a viable cell density of 3.7×10(7) cells/ml and produced chimeric antibody to a maximum value of 60 μg/ml in 120 ml scale vessel. X63Ag8.653 transfectant reached a density of 1.9×10(7) cells/ml in 1.2 I scale vessel in serum supplemented medium (10% FCS-eRDF) and produced chimeric antibody which consisted of chimeric gamma and chimeric kappa chains to a maximum value of 5.8 μg/ml.
authors.affiliation: Biotechnology Research Laboratory, Teijin Limited, 191, Hino, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Takazawa
authors.forename: M
authors.initials: M
authors.lastname: Tokashiki