title: [Analysis of subgroups of lymphocyte in peripheral blood among dermatitis medicamentosa-like of trichloroethylene patients and healthy exposed workers].
abstract: OBJECTIVE
  To study the effects of trichloroethylene (TCE) to lymphocyte subsets among exposed workers, and explore the early immunological effect biomarkers for prevention of hypersensitivity dermatitis induced by TCE.
  
  
  METHODS
  Twenty-eight patients with TCE-induced hypersensitivity dermatitis, 56 healthy TCE-exposed workers from the same workshops with patients, and 28 comparable unexposed controls were recruited in this study. The total lymphocyte count and the major lymphocyte subsets including T cell, CD4(+) T cell, CD8(+) T cell, B cell, NK cell in peripheral blood were measured by Flow Cytometer analysis and Standard blood count analysis.
  
  
  RESULTS
  The total lymphocyte count and T cell, CD4(+) T cell, CD8(+) T cell among patients (median at 2810.00, 1846.17, 831.87, 904.05 cell counts/µl blood) were significantly increased compared with TCE-exposed workers (median at 2101.00, 1218.59, 643.87, 482.81 cell counts/µl blood, Z = -3.19, -4.96, -3.22, -4.99, P < 0.001) and unexposed controls (median at 1900.00, 1223.60, 558.60, 325.80 cell counts/µl blood, Z = -3.30, -4.46, -3.45, -5.03, P < 0.001), the NK cell and CD3(+)CD4(+)/CD3(+)CD8(+) ratio among patients (median at 255.50 cell counts/µl blood and 1.11) were significantly decreased compared with the unexposed controls (median at 642.60 cell counts/µl blood and 1.96, Z = -3.56 and -3.11, P < 0.01). Meanwhile, for the exposed workers, the CD8(+) T cell (median at 482.81 cell counts/µl blood) was significantly increased and the NK cell and CD3(+)CD4(+)/CD3(+)CD8(+) ratio (median at 318.76 cell counts/µl blood and 1.27) were significantly decreased compared with unexposed controls (median at 325.80 and 642.60 cell counts/µl blood and 1.96, Z = -2.63, -3.52, -2.29, P < 0.05).
  
  
  CONCLUSIONS
  Occupational exposure to TCE could affect the lymphocyte subsets, especially T cell and NK cell. The total lymphocyte count, T cell and CD4(+) T cell might be effect biomarkers for subjects with hypersensitivity dermatitis among TCE-exposed workers.
authors.affiliation: Key Laboratory of Chemical Safety and Health, Chinese Center for Disease Control and Prevention; National Institute for Occupational Health and Poison Control, Beijing 100050, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Yi
authors.forename: Yan-xia
authors.initials: YX
authors.lastname: Teng
authors.forename: Dan
authors.initials: D
authors.lastname: Zang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.forename: Hai-yan
authors.initials: HY
authors.lastname: Dong
authors.forename: Yong
authors.initials: Y
authors.lastname: Niu
authors.forename: Ping
authors.initials: P
authors.lastname: Bin
authors.forename: Xian-qing
authors.initials: XQ
authors.lastname: Huang
authors.forename: Yu-xin
authors.initials: YX
authors.lastname: Zheng
authors.forename: Yu-fei
authors.initials: YF
authors.lastname: Dai
mesh_terms: D000293:Adolescent; D000328:Adult; D009783:Dermatitis, Occupational / Q000097:blood / Q000276:immunology*; D003875:Drug Eruptions / Q000097:blood / Q000209:etiology / Q000276:immunology*; D005260:Female; D006801:Humans; D018655:Lymphocyte Count; D016131:Lymphocyte Subsets; D008297:Male; D008875:Middle Aged; D014241:Trichloroethylene / Q000009:adverse effects*; D055815:Young Adult
chemical_list: D014241:Trichloroethylene