title: The impairment of reproduction in db/db mice is not mediated by intraovarian defective leptin signaling.
abstract: OBJECTIVE
  To demonstrate whether leptin modulates reproduction by a direct effect within the ovary.
  
  
  METHODS
  Animal model.
  
  
  METHODS
  National Key Laboratory of Infertility.
  
  
  METHODS
  Adult female db/db mice.
  
  
  METHODS
  Adult littermate wild-type (WT) and diabetic (db) leptin receptor (LR) mutant female mice were matched for the allograft of the ovary to construct new genotypic models, respectively. WT mouse received only one ovary from a WT or a db/db mouse (WT Ov-WT, WT Ov-db), and db/db mouse received one ovary from a WT or a db/db mouse (db Ov-WT, db Ov-db). WT and db/db mice received one ovary from a WT mouse and another ovary from a db/db mouse (WT Ov-WT/db, db Ov-WT/db) or received two ovaries all from a WT mouse (db Ov-WT/WT).
  
  
  METHODS
  Hormones, lipids, and reverse transcription polymerase chain reaction.
  
  
  RESULTS
  Both WT Ov-WT and WT Ov-db mice presented normal cycles, comparable serum E(2) and FSH levels, and ovarian expressions of the Star, Cyp17, and Cyp19 mRNA, even with different ovary genotypes. In WT Ov-WT/db with hMG stimulation, db ovaries with LR mutation expressed higher Star, Cyp17, Cyp19, Jak2, Stat3, and Pias3 mRNA than in the basal state, whereas WT ovaries with intact LR expressed higher Star, Cyp17, and Cyp19 but divergently lower Jak2, Stat3, and Pias3 levels.
  
  
  CONCLUSIONS
  We confirmed that impairment of reproduction in intact db/db mice is not mediated by intraovary intact/defective leptin signaling even in face of a divergent modulation by gonadotropins.
authors.affiliation: Department of Obstetrics and Gynecology, Key Laboratory and Unit of Infertility in Chinese Medicine, First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin, People's Republic of China.
authors.forename: Yuehui
authors.initials: Y
authors.lastname: Zhang
authors.forename: Min
authors.initials: M
authors.lastname: Hu
authors.forename: Hongxia
authors.initials: H
authors.lastname: Ma
authors.forename: Junwei
authors.initials: J
authors.lastname: Qu
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.forename: Lihui
authors.initials: L
authors.lastname: Hou
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.forename: Xiao-Ke
authors.initials: XK
authors.lastname: Wu
mesh_terms: D000818:Animals; D001141:Aromatase / Q000235:genetics; D003920:Diabetes Mellitus / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D004195:Disease Models, Animal; D004958:Estradiol / Q000097:blood; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood; D005838:Genotype; D053614:Janus Kinase 2 / Q000235:genetics; D020738:Leptin / Q000378:metabolism*; D051379:Mice; D057507:Mice, 129 Strain; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D009154:Mutation; D010053:Ovary / Q000378:metabolism* / Q000503:physiopathology / Q000637:transplantation; D010641:Phenotype; D010750:Phosphoproteins / Q000235:genetics; D050828:Protein Inhibitors of Activated STAT / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D054411:Receptors, Leptin / Q000235:genetics / Q000378:metabolism*; D012098:Reproduction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050796:STAT3 Transcription Factor / Q000235:genetics; D015398:Signal Transduction; D013254:Steroid 17-alpha-Hydroxylase / Q000235:genetics; D013997:Time Factors; D014184:Transplantation, Homologous; D000098682:Steroidogenic Acute Regulatory Protein
chemical_list: D020738:Leptin; D010750:Phosphoproteins; C109279:Pias3 protein, mouse; D050828:Protein Inhibitors of Activated STAT; D012333:RNA, Messenger; D054411:Receptors, Leptin; D050796:STAT3 Transcription Factor; C494087:Stat3 protein, mouse; C515447:leptin receptor, mouse; D000098682:Steroidogenic Acute Regulatory Protein; D004958:Estradiol; D005640:Follicle Stimulating Hormone; D001141:Aromatase; D013254:Steroid 17-alpha-Hydroxylase; C507927:Jak2 protein, rat; D053614:Janus Kinase 2