title: High density culture of mouse-human hybridoma cells using a perfusion culture apparatus with multi-settling zones to separate cells from the culture medium.
abstract: Mouse-human hybridoma X87X cells were cultivated using a novel perfusion culture apparatus provided with three-settling zones to separate the cells from the culture medium by gravitational settling. The maximum viable cell density in a serum-free culture medium attained 3.0×10(7) cells/ml, when the specific perfusion rate was set to 2.3 vol day(-1), and monoclonal antibody was continuously produced. These results were almost the same as those in the perfusion culture vessel with one settling zone and revealed that the process with a plurality of settling zones is a promising one for scale-up of a gravitation type of perfusion culture vessel.
authors.affiliation: Biotechnology Research Laboratories, Tokyo Research Center, Teijin Limited, 4-3-2, Asahigaoka, 191, Hino-shi, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Tokashiki
authors.forename: T
authors.initials: T
authors.lastname: Arai