title: High diversity of Cryptosporidium subgenotypes identified in Malaysian HIV/AIDS individuals targeting gp60 gene.
abstract: BACKGROUND
  Currently, there is a lack of vital information in the genetic makeup of Cryptosporidium especially in developing countries. The present study aimed at determining the genotypes and subgenotypes of Cryptosporidium in hospitalized Malaysian human immunodeficiency virus (HIV) positive patients.
  
  
  RESULTS
  In this study, 346 faecal samples collected from Malaysian HIV positive patients were genetically analysed via PCR targeting the 60 kDa glycoprotein (gp60) gene. Eighteen (5.2% of 346) isolates were determined as Cryptosporidium positive with 72.2% (of 18) identified as Cryptosporidium parvum whilst 27.7% as Cryptosporidium hominis. Further gp60 analysis revealed C. parvum belonging to subgenotypes IIaA13G1R1 (2 isolates), IIaA13G2R1 (2 isolates), IIaA14G2R1 (3 isolates), IIaA15G2R1 (5 isolates) and IIdA15G1R1 (1 isolate). C. hominis was represented by subgenotypes IaA14R1 (2 isolates), IaA18R1 (1 isolate) and IbA10G2R2 (2 isolates).
  
  
  CONCLUSIONS
  These findings highlighted the presence of high diversity of Cryptosporidium subgenotypes among Malaysian HIV infected individuals. The predominance of the C. parvum subgenotypes signified the possibility of zoonotic as well as anthroponotic transmissions of cryptosporidiosis in HIV infected individuals.
authors.affiliation: Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Asma
authors.initials: A
authors.lastname: Iqbal
authors.forename: Yvonne A L
authors.initials: YA
authors.lastname: Lim
authors.forename: Johari
authors.initials: J
authors.lastname: Surin
authors.forename: Benedict L H
authors.initials: BL
authors.lastname: Sim
mesh_terms: D003457:Cryptosporidiosis / Q000145:classification; D003458:Cryptosporidium / Q000235:genetics* / Q000302:isolation & purification; D016054:DNA, Protozoan / Q000235:genetics*; D005243:Feces / Q000469:parasitology; D017125:Genes, Protozoan; D014644:Genetic Variation; D005838:Genotype; D006023:Glycoproteins / Q000235:genetics*; D015658:HIV Infections / Q000150:complications / Q000469:parasitology*; D008296:Malaysia; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D016054:DNA, Protozoan; D006023:Glycoproteins; D014764:Viral Proteins