title: Dynamic and complex Mycobacterium tuberculosis microevolution unrevealed by standard genotyping.
abstract: We performed an in-depth analysis of a large Mycobacterium tuberculosis cluster involving nine isolates with identical or highly similar RFLP types. The strain involved in this transmission cluster microevolved and accumulated up to five differential IS6110 transposition events, several of them not revealed by standard genotyping approaches. Some of these events probably generated extended deletions. In two cases of tuberculosis within the cluster, we observed the simultaneous presence of both the reference and microevolved variants. A longitudinal genotypical survey of M. tuberculosis in a real clinical evolutionary scenario can reveal dynamic and complex microevolution events.
authors.affiliation: Servicio de Microbiología y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, C/ Dr Esquerdo, 46, 28007 Madrid, Spain; Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Pérez-Lago
authors.forename: Marta
authors.initials: M
authors.lastname: Herranz
authors.forename: Emilio
authors.initials: E
authors.lastname: Bouza
authors.forename: Darío
authors.initials: D
authors.lastname: García de Viedma
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods; D016000:Cluster Analysis; D019143:Evolution, Molecular; D005838:Genotype; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D014376:Tuberculosis / Q000382:microbiology* / Q000635:transmission