title: Enhanced gene delivery using Bubble liposomes and ultrasound for folate-PEG liposomes.
abstract: We have previously reported that the transfection efficiency of laminin-derived AG73-peptide labeled polyethyleneglycol-modified liposomes (AG73-PEG liposomes) was enhanced by echo-contrast gas entrapping PEG liposomes (Bubble liposomes, BLs) and ultrasound (US) exposure by improving endosomal escape. However, it has not been well understood whether BLs and US exposure can enhance the transfection efficiency of other carriers except AG73-PEG liposomes. In this study, to evaluate whether BLs and US exposure can be generally applied to gene delivery carriers, we focused on folate as a model ligand and examined whether BLs and US exposure could enhance the transfection efficiency of folate-PEG liposomes. Folate-PEG liposomes could internalize into cells efficiently, whereas they could not deliver genes into cytosol from endosomes sufficiently. BLs and US exposure could enhance the transfection efficiency of folate-PEG liposomes compared with folate-PEG liposomes alone without their direct induction into cells. These results suggested that BLs and US exposure could enhance the transfection efficiency of folate-PEG liposomes in the same manner as AG73-PEG liposomes. Thus, BLs and US exposure may be a promising tool to achieve efficient gene transfection into various gene carriers in general.
authors.affiliation: Department of Drug Delivery and Molecular Biopharmaceutics, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, Japan.
authors.forename: Daiki
authors.initials: D
authors.lastname: Omata
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Negishi
authors.forename: Shoko
authors.initials: S
authors.lastname: Hagiwara
authors.forename: Sho
authors.initials: S
authors.lastname: Yamamura
authors.forename: Yoko
authors.initials: Y
authors.lastname: Endo-Takahashi
authors.forename: Ryo
authors.initials: R
authors.lastname: Suzuki
authors.forename: Kazuo
authors.initials: K
authors.lastname: Maruyama
authors.forename: Yukihiko
authors.initials: Y
authors.lastname: Aramaki
mesh_terms: D045744:Cell Line, Tumor; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D004247:DNA / Q000008:administration & dosage / Q000235:genetics; D004337:Drug Carriers / Q000737:chemistry*; D011992:Endosomes / Q000187:drug effects / Q000378:metabolism; D005434:Flow Cytometry; D005492:Folic Acid / Q000737:chemistry*; D018014:Gene Transfer Techniques; D006801:Humans; D007797:Laminin / Q000008:administration & dosage / Q000737:chemistry / Q000235:genetics; D008081:Liposomes; D008156:Luciferases / Q000235:genetics; D045423:Microbubbles; D018613:Microscopy, Confocal; D010316:Particle Size; D010446:Peptide Fragments / Q000008:administration & dosage / Q000737:chemistry / Q000235:genetics; D010957:Plasmids; D011092:Polyethylene Glycols / Q000737:chemistry*; D014162:Transfection; D014465:Ultrasonics
chemical_list: C105786:AG 73; D004337:Drug Carriers; D007797:Laminin; D008081:Liposomes; D010446:Peptide Fragments; D011092:Polyethylene Glycols; D004247:DNA; D005492:Folic Acid; D008156:Luciferases