title: Production of cellulolytic enzymes containing cinnamic acid esterase from Schizophyllum commune.
abstract: To develop enzyme preparations capable of digesting plant biomass, we examined the production of cinnamic acid esterase as well as cellulolytic and xylanolytic enzymes in cultures of Schizophyllum commune. The cinnamic acid esterase was produced in the cultures containing solid cellulosic substrates, with production being enhanced by delignifying the wood powder. This indicates that these esterases are produced by cellulose, despite their substrates being phenolic compounds. Cellulolytic and xylanolytic enzymes, with the exception of α-arabinofuranosidase, were also produced in cultures containing cellulosic substances. These results show that enzyme preparation can have high activity of cinnamic acid esterase and cellulolytic and xylanolytic enzymes when S. commune is incubated in the presence of cellulose. These enzyme preparations will be useful for digesting plant biomass and for releasing cinnamic acid derivatives from plant cell walls.
authors.affiliation: Department of Environmental Science, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University. s_tsuji@kpu.ac.jp
authors.forename: Sho-ichi
authors.initials: S
authors.lastname: Tsujiyama
authors.forename: Hitomi
authors.initials: H
authors.lastname: Ueno
mesh_terms: D018533:Biomass; D002482:Cellulose / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D004950:Esterases / Q000302:isolation & purification* / Q000378:metabolism*; D010944:Plants / Q000378:metabolism; D012567:Schizophyllum / Q000201:enzymology*; D014934:Wood / Q000378:metabolism
chemical_list: D003470:Culture Media; D002482:Cellulose; D004950:Esterases