title: MicroRNAs regulate B-cell receptor signaling-induced apoptosis.
abstract: Apoptosis induced by B-cell receptor (BCR) signaling is critical for antigen-driven selection, a process critical to tolerance and immunity. Here, we examined the roles of microRNAs (miRNAs) in BCR signaling-induced apoptosis using the widely applied WEHI-231 model. Comparison of miRNA levels in BCR-stimulated and -unstimulated cells revealed that 39 miRNAs were differentially expressed upon stimulation of the BCR. Importantly, stimulation in the presence of anti-CD40 antibodies, which rescues cells from BCR-induced apoptosis, prevented most changes in miRNA expression. Ectopic expression of mir-150 and mir-181a1b1, miRNAs that were upregulated upon BCR stimulation, resulted in inhibition of cell growth. Finally, we showed that ectopic expression of mir-150, mir-181a1b1 and mir-17∼92 sensitized cells to anti-IgM stimulation-induced growth inhibition. Together, these results demonstrate that miRNAs are involved in BCR signaling, suggesting that they may have important roles in the regulation of B cell-mediated tolerance and immunity.
authors.affiliation: Department of Microbiology and Immunology, Baxter laboratory for Stem Cell Biology, Stanford University School of Medicine, Stanford, CA, USA. j.l.kluiver@umcg.nl
authors.forename: J L
authors.initials: JL
authors.lastname: Kluiver
authors.forename: C-Z
authors.initials: CZ
authors.lastname: Chen
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002460:Cell Line; D049109:Cell Proliferation; D016000:Cluster Analysis; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D051379:Mice; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D011947:Receptors, Antigen, B-Cell / Q000276:immunology / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D035683:MicroRNAs; C529101:Mirn150 microRNA, mouse; D011947:Receptors, Antigen, B-Cell; C529100:mirn181 microRNA, mouse