title: Detection and identification of peroxiredoxin 3 as a biomarker in hepatocellular             carcinoma by a proteomic approach.
abstract: Hepatocellular carcinoma (HCC) is one of the most common malignancies worldwide             with poor prognosis due to resistance to conventional chemotherapy and limited             efficacy of radiotherapy. Thus, alternative therapeutic strategies need to be             established. In order to search for a useful biomarker to improve its efficacy,             we conducted a two-dimensional gel electrophoresis and MALDI-TOF MS-based comparative             proteomic analysis to profile the differentially expressed proteins between HCC             tumor tissues with histological evidence and the adjacent non-tumor tissues. Twenty-two             out of 43 dysregulated proteins were identified, including 15 upregulated proteins,             and 7 downregulated proteins (over 2-fold, P<0.01). The expression of peroxiredoxin 3             (PRDX3) at the mRNA and protein levels was confirmed by RT-PCR and western blotting             in HCC cell lines, and HCC samples, and further analysed by immunohistochemistry             in HCC samples of different clinical pathological stages. The results indicated             that overexpression of PRDX3 was associated with 94.9% HCC, and correlated with             poor differentiation (P<0.05), which suggest that PRDX3 has substantial clinical             impact on the progression of hepatocarcinoma, and may be a potential therapeutic             target for HCC.
authors.affiliation: Department of Surgery, The Third Xiangya Hospital, Central South                     University, Changsha, Hunan 410013, P.R. China.
authors.forename: Bingbing
authors.initials: B
authors.lastname: Qiao
authors.forename: Jiajia
authors.initials: J
authors.lastname: Wang
authors.forename: Jianfei
authors.initials: J
authors.lastname: Xie
authors.forename: Ying
authors.initials: Y
authors.lastname: Niu
authors.forename: Shaojun
authors.initials: S
authors.lastname: Ye
authors.forename: Qiquan
authors.initials: Q
authors.lastname: Wan
authors.forename: Qifa
authors.initials: Q
authors.lastname: Ye
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics*; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008113:Liver Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D061105:Peroxiredoxin III / Q000032:analysis / Q000235:genetics*; D020543:Proteome / Q000032:analysis / Q000235:genetics; D040901:Proteomics / Q000379:methods*; D012333:RNA, Messenger / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D014408:Biomarkers, Tumor; D020543:Proteome; D012333:RNA, Messenger; D061105:Peroxiredoxin III