title: Live cell imaging in live animals with fluorescent proteins.
abstract: The discovery, cloning, and characterization of GFP and related proteins of many colors have enabled live cell imaging to an unprecedented extent and resolution. Essentially, any cellular process can be imaged with a fluorescent protein. These proteins serve as genetic reporters and therefore can be used to follow cellular processes over indefinite periods in vivo as well as in vitro. The brightness and specific spectra of fluorescent proteins allow them to be imaged in vivo, using specific filters, without interference from autofluorescence. This chapter describes the development of live imaging in live animals with subcellular resolution, emphasizing the study of in vivo cell biology of cancer growth, spread, and metastasis.
authors.affiliation: Department of Surgery, University of California San Diego, San Diego, California, USA.
authors.forename: Robert M
authors.initials: RM
authors.lastname: Hoffman
mesh_terms: D000818:Animals; D006651:Histocytochemistry / Q000379:methods; D006801:Humans; D049408:Luminescent Agents / Q000032:analysis*; D008164:Luminescent Proteins / Q000032:analysis*; D051379:Mice; D008819:Mice, Nude; D008822:Mice, Transgenic; D008856:Microscopy, Fluorescence / Q000379:methods*; D009362:Neoplasm Metastasis / Q000473:pathology / Q000648:ultrastructure; D009369:Neoplasms / Q000098:blood supply / Q000473:pathology*; D009360:Neoplastic Cells, Circulating / Q000473:pathology / Q000648:ultrastructure; D009389:Neovascularization, Pathologic / Q000473:pathology
chemical_list: D049408:Luminescent Agents; D008164:Luminescent Proteins