title: Circulating factors induce coronary endothelial cell activation following exposure to inhaled diesel exhaust and nitrogen dioxide in humans: evidence from a novel translational in vitro model.
abstract: The vascular toxicity of inhaled agents may be caused by soluble factors that are released into the systemic circulation. To confirm this in a straightforward manner, we obtained plasma from healthy human volunteers before and after exposure to diesel exhaust (DE) and nitrogen dioxide (NO(2)). Plasma samples were obtained from human volunteers exposed to 100 μg/m(3) DE or filtered air for 2 h. A second cohort was exposed to 500 ppb NO(2) or filtered air in an identical protocol. Primary human coronary artery endothelial cells (hCAECs) were grown to confluence and treated for 24 h with a 10 or 30% (in media) mixture of plasma obtained before, immediately post or 24 h postexposure to pollutant exposures. Messenger RNA (mRNA) was isolated from hCAECs following the incubation and probed for intracellular cell adhesion molecule (ICAM-1) and vascular cell adhesion molecule (VCAM-1) expression. ICAM-1 mRNA expression was increased by plasma obtained at both timepoints following the NO(2) exposures. VCAM-1 was significantly elevated in cells treated with plasma obtained 24 h following diesel exposure and at both timepoints following NO(2) exposure. Interleukin-8 protein was elevated in the hCAEC supernatant when cells were incubated with plasma from NO(2) exposures. These data indicate that proinflammatory circulating factors are elevated acutely following exposure to both DE and a primary component thereof, NO(2). These functional translational assays offer novel approaches to assessing the cardiovascular risk associated with air pollution exposure.
authors.affiliation: Department of Pharmaceutical Sciences, College of Pharmacy, University of New Mexico, Albuquerque, New Mexico 87131, USA.
authors.forename: Meghan M
authors.initials: MM
authors.lastname: Channell
authors.forename: Michael L
authors.initials: ML
authors.lastname: Paffett
authors.forename: Robert B
authors.initials: RB
authors.lastname: Devlin
authors.forename: Michael C
authors.initials: MC
authors.lastname: Madden
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Campen
mesh_terms: D000393:Air Pollutants / Q000633:toxicity*; D002478:Cells, Cultured; D003331:Coronary Vessels / Q000187:drug effects* / Q000378:metabolism; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D018836:Inflammation Mediators / Q000494:pharmacology*; D019570:Inhalation Exposure; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism; D009585:Nitrogen Dioxide / Q000633:toxicity*; D011014:Pneumonia / Q000097:blood / Q000139:chemically induced*; D012333:RNA, Messenger / Q000378:metabolism; D019010:Vascular Cell Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism; D001335:Vehicle Emissions / Q000633:toxicity*
chemical_list: D000393:Air Pollutants; D018836:Inflammation Mediators; D012333:RNA, Messenger; D019010:Vascular Cell Adhesion Molecule-1; D001335:Vehicle Emissions; D018799:Intercellular Adhesion Molecule-1; D009585:Nitrogen Dioxide