title: Lipase-coated K2SO4 micro-crystals: preparation, characterization, and application in biodiesel production using various oil feedstocks.
abstract: This study investigated the preparation and characteristics of protein-coated microcrystals (PCMCs) from Pseudomonas cepacia lipase (PS) and K(2)SO(4), and their application in biodiesel synthesis, via single factorial experiments and response surface methodology (RSM), the optimized PCMC-PS exhibited high activity and stability; the optimal temperature was 60°C (which gave 99.83% conversion), although fairly high activity was exhibited after incubation at different temperatures (25-70°C). The organic solvents stability of the PCMC-PS was improved, and it significantly reduced ethanol toxicity. Circular dichroism (CD) analysis revealed the correlation between the conformation and the enzyme activity. The morphology of the PCMC-PS was also confirmed via scanning electron microscopy (SEM). When catalyzed by PCMC-PS, above 83% biodiesel yield was obtained for most of the seven oils tested. The PCMC-PS (washed with hexane) activity remained relatively stable after eight batch reactions, with only a 15.73% reduction in the conversion (from 99.02% to 83.29%).
authors.affiliation: Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, PR China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Zheng
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.forename: Yun
authors.initials: Y
authors.lastname: Liu
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zhang
authors.forename: Yunjun
authors.initials: Y
authors.lastname: Yan
mesh_terms: D056804:Biofuels; D002849:Chromatography, Gas; D003460:Crystallization; D008049:Lipase / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D013431:Sulfates / Q000737:chemistry*; D013696:Temperature
chemical_list: D056804:Biofuels; D013431:Sulfates; C031512:potassium sulfate; D008049:Lipase