title: Selection of transgenic rice plants using a herbicide tolerant form of the acetolactate synthase gene.
abstract: Acetolactate synthase (ALS) is an enzyme in the biosynthetic pathway for branched-chain amino acids, and bispyribac-sodium (BS), a pyrimidinyl carboxy herbicide, is a well-known inhibitor of ALS activity. However, it appears that a mutated form of rice ALS [OsmALS (W548L/S627I)] confers resistance to BS. We succeeded in using OsmALS with native OsALS promoter and terminator region for a selection marker of rice transformation. Because this selection marker cassette is originally from the rice endogenous genome, it can be expected to be publicly acceptable.
authors.affiliation: Plant Genetic Engineering Research Unit, National Institute of Agrobiological Sciences, Tsukuba, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Endo
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Shimizu
authors.forename: Seiichi
authors.initials: S
authors.lastname: Toki
mesh_terms: D000095:Acetolactate Synthase / Q000235:genetics*; D001565:Benzoates / Q000494:pharmacology; D053209:Herbicide Resistance / Q000235:genetics*; D006540:Herbicides / Q000494:pharmacology; D009154:Mutation; D012275:Oryza / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000235:genetics; D011401:Promoter Regions, Genetic; D011743:Pyrimidines / Q000494:pharmacology
chemical_list: D001565:Benzoates; D006540:Herbicides; D011743:Pyrimidines; C549011:bispyribac; D000095:Acetolactate Synthase