title: Gene expression profiling through microarray analysis in Arabidopsis thaliana colonized by Pseudomonas putida MTCC5279, a plant growth promoting rhizobacterium.
abstract: Plant growth promotion is a multigenic process under the influence of many factors; therefore an understanding of these processes and the functions regulated may have profound implications. Present study reports microarray analysis of Arabidopsis thaliana plants inoculated with Pseudomonas putida MTCC5279 (MTCC5279) which resulted in significant increase in growth traits as compared with non-inoculated control. The gene expression changes, represented by oligonucleotide array (24652 genes) have been studied to gain insight into MTCC5279 assisted plant growth promotion in Arabidopsis thaliana. MTCC5279 induced upregulated Arabidopsis thaliana genes were found to be involved in maintenance of genome integrity (At5g20850), growth hormone (At3g23890 and At4g36110), amino acid synthesis (At5g63890), abcissic acid (ABA) signaling and ethylene suppression (At2g29090, At5g17850), Ca⁺² dependent signaling (At3g57530) and induction of induced systemic resistance (At2g46370, At2g44840). The genes At3g32920 and At2g15890 which are suggested to act early in petal, stamen and embryonic development are among the downregulated genes. We report for the first time MTCC5279 assisted repression of At3g32920, a putative DNA repair protein involved in recombination and DNA strand transfer in a process of rapid meiotic and mitotic division.
authors.affiliation: CSIR-National Botanical Research Institute, Lucknow, India.
authors.forename: Suchi
authors.initials: S
authors.lastname: Srivastava
authors.forename: Vasvi
authors.initials: V
authors.lastname: Chaudhry
authors.forename: Aradhana
authors.initials: A
authors.lastname: Mishra
authors.forename: Puneet Singh
authors.initials: PS
authors.lastname: Chauhan
authors.forename: Ateequr
authors.initials: A
authors.lastname: Rehman
authors.forename: Archana
authors.initials: A
authors.lastname: Yadav
authors.forename: Narendra
authors.initials: N
authors.lastname: Tuteja
authors.forename: Chandra S
authors.initials: CS
authors.lastname: Nautiyal
mesh_terms: D017360:Arabidopsis / Q000382:microbiology / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000494:pharmacology*; D018744:DNA, Plant / Q000187:drug effects; D015870:Gene Expression; D020869:Gene Expression Profiling / Q000379:methods; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D017343:Genes, Plant / Q000187:drug effects*; D006133:Growth Substances / Q000235:genetics* / Q000494:pharmacology; D008540:Meiosis; D046228:Microarray Analysis / Q000379:methods; D008938:Mitosis; D016958:Pseudomonas putida / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D001426:Bacterial Proteins; D018744:DNA, Plant; D006133:Growth Substances