title: Exploration of beer proteome using OFFGEL prefractionation in combination with two-dimensional gel electrophoresis with narrow pH range gradients.
abstract: Two-dimensional gel electrophoresis in combination with mass spectrometry has already been applied successfully to study beer proteome. Due to the abundance of protein Z in beer samples, prefractionation techniques might help to improve beer proteome coverage. Proteins from four lager beers of different origins were separated by two-dimensional electrophoresis (2-DE) followed by tandem mass spectrometric analysis. Initially 52 proteins mostly from  Hordeum vulgare  (22 proteins) and Saccharomyces species (25 proteins) were identified. Preparative isoelectric focusing by OFFGEL Fractionator was applied prior to 2-DE to improve its resolution power. As a result of this combined approach, a total of 70 beer proteins from Hordeum vulgare (30 proteins), from  Saccharomyces  species (31 proteins), and from other sources (9 proteins) were identified. Of these, 37 proteins have not been previously reported in beer samples.
authors.affiliation: Core Facility-Proteomics, Central European Institute of Technology, Masaryk University, Brno, Czech Republic.
authors.forename: Hana
authors.initials: H
authors.lastname: Konečná
authors.forename: Lukáš
authors.initials: L
authors.lastname: Müller
authors.forename: Hana
authors.initials: H
authors.lastname: Dosoudilová
authors.forename: David
authors.initials: D
authors.lastname: Potěšil
authors.forename: Jana
authors.initials: J
authors.lastname: Buršíková
authors.forename: Ondrej
authors.initials: O
authors.lastname: Sedo
authors.forename: Ivana
authors.initials: I
authors.lastname: Márová
authors.forename: Zbyněk
authors.initials: Z
authors.lastname: Zdráhal
mesh_terms: D001515:Beer / Q000032:analysis* / Q000382:microbiology; D005591:Chemical Fractionation / Q000379:methods*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000295:instrumentation / Q000379:methods*; D005656:Fungal Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001467:Hordeum / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D013058:Mass Spectrometry; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D020543:Proteome / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D018892:Proton-Motive Force; D012440:Saccharomyces / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D010940:Plant Proteins; D020543:Proteome