title: Effects of acute and chronic inflammation on proteins involved in duodenal iron absorption in mice: a time-course study.
abstract: In order to understand better the molecular mechanisms involved in the pathogenesis of anaemia of inflammation, we carried out a time-course study on the effects of turpentine-induced acute and chronic inflammation on duodenal proteins involved in Fe absorption in mice. Expression levels of these proteins and hepatic hepcidin and serum Fe levels were determined in inflamed mice. In acutely inflamed mice, significantly increased expression of ferritin was the earliest change observed, followed by decreased divalent metal transporter 1 expression in the duodenum and increased hepcidin expression in the liver. Ferroportin expression increased subsequently, despite high levels of hepcidin. Hypoferraemia, which developed at early time periods studied, was followed by increased serum Fe levels at later points. The present results thus show that acute inflammation induced several changes in the expression of proteins involved in duodenal Fe absorption, contributing to the development of hypoferraemia. Resolution of inflammation caused attenuation of many of these effects. Effects in chronically inflamed mice were less consistent. The present results also suggest that inflammation-induced increases in ferritin appeared to override the effects of hepcidin on the expression levels of ferroportin in enterocytes.
authors.affiliation: Department of Biochemistry, Christian Medical College, Vellore, Tamil Nadu, India.
authors.forename: Abitha
authors.initials: A
authors.lastname: Sukumaran
authors.forename: Joe
authors.initials: J
authors.lastname: Varghese
authors.forename: Jesintha
authors.initials: J
authors.lastname: Tamilselvan
authors.forename: Visalakshi
authors.initials: V
authors.lastname: Jeyaseelan
authors.forename: Thenmozhi
authors.initials: T
authors.lastname: Mani
authors.forename: Robert James
authors.initials: RJ
authors.lastname: Simpson
authors.forename: Andrew Tristan
authors.initials: AT
authors.lastname: McKie
authors.forename: Molly
authors.initials: M
authors.lastname: Jacob
mesh_terms: D000208:Acute Disease; D018798:Anemia, Iron-Deficiency / Q000209:etiology*; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000235:genetics / Q000378:metabolism; D027682:Cation Transport Proteins / Q000235:genetics / Q000378:metabolism; D002908:Chronic Disease; D004382:Duodenitis / Q000097:blood / Q000276:immunology / Q000378:metabolism* / Q000503:physiopathology; D004386:Duodenum / Q000276:immunology / Q000378:metabolism*; D005293:Ferritins / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D064451:Hepcidins; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000276:immunology / Q000378:metabolism*; D019266:Iron, Dietary / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors; D000098742:Ferroportin; D000099236:Solute Carrier Family 11, Member 2
chemical_list: D023181:Antimicrobial Cationic Peptides; D027682:Cation Transport Proteins; D005293:Ferritins; D064451:Hepcidins; D019266:Iron, Dietary; D012333:RNA, Messenger; D000098742:Ferroportin; D000099236:Solute Carrier Family 11, Member 2; C579599:Hamp protein, mouse