title: [Study on the genotypes of 260 Mycobacterium tuberculosis isolates by Spoligotyping method in Jiangsu province, China].
abstract: OBJECTIVE
  To study the genotypes of Mycobacterium tuberculosis (M. tuberculosis) strains isolated from Jiangsu province and to explore the relationship between the 'Beijing family' and the drug resistance of M. tuberculosis.
  
  
  METHODS
  Two hundred and sixty M. tuberculosis strains were isolated from 30 drug surveillance sites in Jiangsu province. Susceptibility of the isolates to the first-line antituberculosis drugs (isoniazid, streptomycin, rifampicin and ethambutol) was tested by using the proportion method. Molecular typing of M. tuberculosis strains was determined by Spoligotyping and analyzed with BioNumerics software.
  
  
  RESULTS
  Based on Spoligotyping fingerprint, 260 strains showed 34 different genotypes, including 27 exclusive genotypes and 7 shared genotypes. These strains could be clustered into two groups: the Beijing family (80.4%, 209/260) and the Non-Beijing family (19.6%, 51/260). Data from logistic regression analysis revealed that infection by the Beijing family was related to an increased risk of multi-drug resistant M. tuberculosis, with the OR (95%CI) of 11.07 (1.45 - 84.50). Non-Beijing families including T1, T2, H3, H4, CAS, LAM, U and MANU2 families were also found. Among them, the CAS, LAM and MANU2 families were first reported in Jiangsu province.
  
  
  CONCLUSIONS
  It was revealed that the marked gene polymorphisms did exist in M. tuberculosis strains. The Beijing family had been the predominant strain circulating in Jiangsu province, which might be related to multi-drug resistant M. tuberculosis strains.
authors.affiliation: Department of Epidemiology and Biostatistics, Nanjing Medical University, Nanjing, China.
authors.forename: Qiao
authors.initials: Q
authors.lastname: Liu
authors.forename: Wei-guo
authors.initials: WG
authors.lastname: Xu
authors.forename: Kang-lin
authors.initials: KL
authors.lastname: Wan
authors.forename: Jian-ming
authors.initials: JM
authors.lastname: Wang
authors.forename: Dan-dan
authors.initials: DD
authors.lastname: Yang
authors.forename: Bing
authors.initials: B
authors.lastname: Lü
authors.forename: Yan
authors.initials: Y
authors.lastname: Shao
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
mesh_terms: D000995:Antitubercular Agents / Q000494:pharmacology*; D015373:Bacterial Typing Techniques; D002681:China / Q000453:epidemiology; D024881:Drug Resistance, Bacterial; D005838:Genotype; D006801:Humans; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000187:drug effects* / Q000235:genetics*; D011110:Polymorphism, Genetic; D012293:Rifampin / Q000494:pharmacology; D014376:Tuberculosis / Q000453:epidemiology / Q000382:microbiology
chemical_list: D000995:Antitubercular Agents; D012293:Rifampin