title: Discrimination of the oral microbiota associated with high hydrogen sulfide and methyl mercaptan production.
abstract: Both hydrogen sulfide (H2S) and methyl mercaptan (CH(3)SH) are frequently detected in large amounts in malodorous mouth air. We investigated the bacterial composition of saliva of 30 subjects with severe oral malodor exhibiting extreme CH(3)SH/H(2)S ratios (high H(2)S but low CH(3)SH concentrations, n 5 14; high CH(3)SH but low H2S concentrations, n 5 16) and 13 subjects without malodor, using barcoded pyrosequencing analysis of the 16S rRNA gene. Phylogenetic community analysis with the UniFrac distance metric revealed a distinct bacterial community structure in each malodor group. The H2S group showed higher proportions of the genera Neisseria, Fusobacterium, Porphyromonas and SR1 than the other two groups, whereas the CH(3)SH group had higher proportions of the genera Prevotella, Veillonella,Atopobium, Megasphaera, and Selenomonas. Our results suggested that distinct bacterial populations in the oral microbiota are involved in production of high levels of H2S and CH3SH in the oral cavity.
authors.affiliation: Section of Preventive Dentistry, Division of Oral Health, Growth and Development, Kyushu University Faculty of Dental Science, Fukuoka, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Takeshita
authors.forename: Nao
authors.initials: N
authors.lastname: Suzuki
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Nakano
authors.forename: Masaki
authors.initials: M
authors.lastname: Yasui
authors.forename: Masahiro
authors.initials: M
authors.lastname: Yoneda
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Shimazaki
authors.forename: Takao
authors.initials: T
authors.lastname: Hirofuji
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Yamashita
mesh_terms: D000293:Adolescent; D000328:Adult; D001419:Bacteria / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D001483:Base Sequence; D017931:DNA Primers; D005260:Female; D006209:Halitosis / Q000382:microbiology*; D006801:Humans; D006862:Hydrogen Sulfide / Q000378:metabolism*; D008297:Male; D054892:Metagenome; D009055:Mouth / Q000382:microbiology*; D010802:Phylogeny; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D055815:Young Adult
chemical_list: D017931:DNA Primers; D013438:Sulfhydryl Compounds; C005231:methylmercaptan; D006862:Hydrogen Sulfide