title: Development of a transcription-reverse transcription concerted reaction method for specific detection of human enterovirus 71 from clinical specimens.
abstract: A transcription-reverse transcription (RT) concerted reaction (TRCR) method was developed for rapid and specific detection of EV71 from clinical specimens. This method was validated with EV71 strains from all of the known genotypes (genotypes A, B1 to B5, and C1 to C5), with detection limits of 10 to 10(3) copies, and was useful for identification of EV71 from throat swabs of patients with hand, foot, and mouth disease (HFMD).
authors.affiliation: Tokyo Research Center, Tosoh Corp., Hayakawa, Ayase City, Kanagawa, Japan.
authors.forename: Naoto
authors.initials: N
authors.lastname: Nakajima
authors.forename: Yuka
authors.initials: Y
authors.lastname: Kitamori
authors.forename: Satoru
authors.initials: S
authors.lastname: Ohnaka
authors.forename: Yasutami
authors.initials: Y
authors.lastname: Mitoma
authors.forename: Katsumi
authors.initials: K
authors.lastname: Mizuta
authors.forename: Takaji
authors.initials: T
authors.lastname: Wakita
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Shimizu
authors.forename: Minetaro
authors.initials: M
authors.lastname: Arita
mesh_terms: D029821:Enterovirus A, Human / Q000235:genetics / Q000302:isolation & purification*; D006232:Hand, Foot and Mouth Disease / Q000175:diagnosis* / Q000821:virology; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D010614:Pharynx / Q000821:virology; D012367:RNA, Viral / Q000235:genetics*; D048348:Reverse Transcription; D012680:Sensitivity and Specificity; D014158:Transcription, Genetic; D014773:Virology / Q000379:methods*
chemical_list: D012367:RNA, Viral