title: Porcine endogenous retrovirus copy number in different pig breeds is not related to genetic diversity.
abstract: The risk of zoonoses is a major obstacle to xenotransplantation. Porcine endogenous retrovirus (PERV) poses a potential risk of zoonotic infection, and its control is a prerequisite for the development of clinical xenotransplantation. The copy number of PERV varies among different breeds, and it has been suggested that the PERV integrations number is increased by inbreeding. The purpose of this study was (i) to examine the copy number of PERV in different Spanish pig breeds, Spanish wild boar and commercial cross-bred pigs from five different farms and genetic background (CCP1-CCP5) and (ii) to investigate the correlation between PERV copy number and the genetic background of the pigs in order to improve the selection of pigs for xenotransplantation. PERV copy number was determined by quantitative, real-time polymerase chain reactions. Thirty-four microsatellite markers were genotyped to describe the genetic diversity within populations (observed and expected heterozygosities, Ho and He, respectively) and the inbreeding coefficient (F). Pearson's correlation coefficient was used to determine the relationship between PERV copy number and Ho, He and F. The copy number of PERV among different pig breeds was estimated to range between three (CCP1) and 43 copies (Iberian Pig). Statistical differences were found among the studied populations concerning PERV copy number. No correlation was found between the PERV copy number and the heterozygosity (calculated at an individual level or at a population level) or the inbreeding coefficient of each population. Our data suggest that pigs inbreeding does not increase PERV copy number and support the idea that careful selection of pigs for organ donation with reduced PERV copy number will minimize the risk of retrovirus transmission to the human receptor.
authors.affiliation: Departamento de Producción Animal, Facultad de Veterinaria, Universidad de Murcia, Murcia, Spain. jjquere@um.es
authors.forename: J J
authors.initials: JJ
authors.lastname: Quereda
authors.forename: J M
authors.initials: JM
authors.lastname: Herrero-Medrano
authors.forename: J M
authors.initials: JM
authors.lastname: Abellaneda
authors.forename: O
authors.initials: O
authors.lastname: García-Nicolás
authors.forename: L
authors.initials: L
authors.lastname: Martínez-Alarcón
authors.forename: F J
authors.initials: FJ
authors.lastname: Pallarés
authors.forename: P
authors.initials: P
authors.lastname: Ramírez
authors.forename: A
authors.initials: A
authors.lastname: Muñoz
authors.forename: G
authors.initials: G
authors.lastname: Ramis
mesh_terms: D000483:Alleles; D000818:Animals; D056915:DNA Copy Number Variations / Q000235:genetics*; D020077:Endogenous Retroviruses / Q000235:genetics*; D015751:Genes, pol / Q000235:genetics*; D014644:Genetic Variation / Q000235:genetics*; D005838:Genotype; D006801:Humans; D007178:Inbreeding; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D010802:Phylogeny; D013552:Swine; D013553:Swine Diseases / Q000821:virology; D014183:Transplantation, Heterologous; D015047:Zoonoses