title: Evaluation of glutathione S-transferase P1 polymorphisms (Ile105Val and Ala114Val) in patients with small cell lung cancer.
abstract: OBJECTIVE
  Glutathione S-transferase P1 (GSTP1) plays an important role in cellular protection against oxidative stress and toxic chemicals. Polymorphisms within GSTP1 are associated with alterations in enzyme activity, which may lead to development of lung disease and cancer. In this study, we aimed to investigate the GSTP1 Ile105Val and Ala114Val polymorphisms in patients with small cell lung cancer (SCLC).
  
  
  METHODS
  GSTP1 Ile105Val polymorphism in exon 5 and GSTP1 Ala114Val polymorphism in exon 6 were determined by using polymerase chain reaction-restriction fragment length polymorphism techniques in 89 patients with SCLC and 108 control patients with chronic obstructive pulmonary disease (COPD). Genotype frequencies and cigarette smoking intensities were compared among SCLC and COPD patients.
  
  
  RESULTS
  There were significantly less SCLC patients with variant exon 6 genotypes than COPD patients (7.9% vs. 20.4%, p=0.007), while the number of patients with variant exon 5 genotypes were comparable among groups. SCLC and COPD patients with variant exon 6 genotype showed trends toward exhibiting reduced cigarette consumption.
  
  
  CONCLUSIONS
  The variant GSTP1 exon 6 genotype might be conferring protection against SCLC development. Whether this effect is associated with exposure to cigarette smoking needs to be clarified.
authors.affiliation: Department of Genetics, Institute for Experimental Medicine (DETAE), Istanbul University, Istanbul, Turkey. vburcak@istanbul.edu.tr
authors.forename: Burcak
authors.initials: B
authors.lastname: Vural
authors.forename: Fatih
authors.initials: F
authors.lastname: Yakar
authors.forename: Duygu
authors.initials: D
authors.lastname: Derin
authors.forename: Pınar
authors.initials: P
authors.lastname: Saip
authors.forename: Aysun
authors.initials: A
authors.lastname: Yakar
authors.forename: Ayşe
authors.initials: A
authors.lastname: Demirkan
authors.forename: Aydın
authors.initials: A
authors.lastname: Karabulut
authors.forename: Elif
authors.initials: E
authors.lastname: Ugurel
authors.forename: Naci
authors.initials: N
authors.lastname: Cine
authors.forename: Zeki
authors.initials: Z
authors.lastname: Kilicaslan
authors.forename: Erdem
authors.initials: E
authors.lastname: Tüzün
authors.forename: Ugur
authors.initials: U
authors.lastname: Ozbek
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019943:Amino Acid Substitution; D005091:Exons / Q000235:genetics; D005260:Female; D051549:Glutathione S-Transferase pi / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008175:Lung Neoplasms / Q000201:enzymology / Q000453:epidemiology / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length; D029424:Pulmonary Disease, Chronic Obstructive / Q000201:enzymology / Q000453:epidemiology / Q000235:genetics; D055752:Small Cell Lung Carcinoma / Q000201:enzymology / Q000453:epidemiology / Q000235:genetics*; D012907:Smoking / Q000009:adverse effects / Q000453:epidemiology / Q000235:genetics; D014421:Turkey / Q000453:epidemiology
chemical_list: C496556:GSTP1 protein, human; D051549:Glutathione S-Transferase pi