title: Different pattern of changes in calcium binding proteins immunoreactivity in the medial prefrontal cortex of rats exposed to stress models of depression.
abstract: Reductions in the number and size of neurons in the medial prefrontal cortex (mPFC) have been documented in many post-mortem studies of depressed patients and animals exposed to stress. Here, we examined the effect of chronic unpredictable stress (CUS) and chronic mild stress (CMS) on specific populations of neurons in the rat mPFC. Antibodies directed against parvalbumin (PV), calbindin D-28K (CB) and active caspase-3 have been used to quantify the numerical density of PV-immunoreactive (PV-ir), CB-ir and active caspase-3-ir cells, and to measure the relative optical density of neuropil. CUS decreased the density of CB-ir neurons and the optical density of CB-ir neuropil. In turn, CMS increased the densities of both CB-ir neurons and neuropil, while PV-ir neurons and PV-ir neuropil were not changed. The frequency distribution of neuronal surface areas was significantly different only for PV-ir neurons, and only between the control and CUS group. CMS reduced the density of active caspase-3-ir cells while CUS did not. We concluded that the mPFC reveals a different pattern of changes in neurons containing calcium binding proteins and active caspase-3 immunoreactivity in response to CUS and CMS.
authors.affiliation: Chair of Pharmacobiology, Jagiellonian University, Collegium Medicum, Medyczna 9, PL 30-688 Kraków, Poland. mfzadroz@cyf-kr.edu.pl
authors.forename: Monika
authors.initials: M
authors.lastname: Zadrożna
authors.forename: Barbara
authors.initials: B
authors.lastname: Nowak
authors.forename: Magdalena
authors.initials: M
authors.lastname: Łasoń-Tyburkiewicz
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Wolak
authors.forename: Magdalena
authors.initials: M
authors.lastname: Sowa-Kućma
authors.forename: Mariusz
authors.initials: M
authors.lastname: Papp
authors.forename: Grażyna
authors.initials: G
authors.lastname: Ossowska
authors.forename: Andrzej
authors.initials: A
authors.lastname: Pilc
authors.forename: Gabriel
authors.initials: G
authors.lastname: Nowak
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism*; D003863:Depression / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D008297:Male; D009474:Neurons / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D017397:Prefrontal Cortex / Q000737:chemistry* / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D013315:Stress, Psychological / Q000150:complications / Q000378:metabolism* / Q000473:pathology
chemical_list: D002135:Calcium-Binding Proteins