title: 5-Lipoxygenase and cyclooxygenase-2 in the lungs of pigs naturally affected by enzootic pneumonia and porcine pleuropneumonia.
abstract: Enzootic pneumonia by Mycoplasma hyopneumoniae and pleuropneumonia by Actinobacillus pleuropneumoniae are among the most common and economically relevant pulmonary diseases in swine herds. We herein investigated the activity and expression of 5-lipoxygenase (5-LOX) and cyclooxygenase-2 (COX-2) in healthy and diseased porcine lungs, by means of immunohistochemical, immunochemical and biochemical assays. Diseased lungs showed a significantly higher activity and expression of 5-LOX and COX-2 in a wide range of cell types, thus suggesting the likely involvement of both enzymes in the pathogenesis of bacterial porcine pneumonia. Consistently, increased enzyme activities were paralleled by increased leukotriene B(4) (LTB(4)), a 5-LOX product and prostaglandin E(2) (PGE(2)), a COX-2 product, content in diseased versus healthy lungs.
authors.affiliation: Department of Comparative Biomedical Sciences, University of Teramo, Piazza A. Moro 45, 64100 Teramo, Italy. rgiacominellistuffler@unite.it
authors.forename: R
authors.initials: R
authors.lastname: Giacominelli-Stuffler
authors.forename: G
authors.initials: G
authors.lastname: Marruchella
authors.forename: M M
authors.initials: MM
authors.lastname: Storelli
authors.forename: A
authors.initials: A
authors.lastname: Sabatucci
authors.forename: C B
authors.initials: CB
authors.lastname: Angelucci
authors.forename: M
authors.initials: M
authors.lastname: Maccarrone
mesh_terms: D000818:Animals; D001094:Arachidonate 5-Lipoxygenase / Q000235:genetics / Q000378:metabolism*; D016718:Arachidonic Acid / Q000378:metabolism; D015153:Blotting, Western; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D011001:Pleuropneumonia / Q000201:enzymology / Q000473:pathology / Q000662:veterinary*; D045729:Pneumonia of Swine, Mycoplasmal / Q000201:enzymology* / Q000473:pathology; D013552:Swine
chemical_list: D016718:Arachidonic Acid; D001094:Arachidonate 5-Lipoxygenase; D051546:Cyclooxygenase 2