title: A new parental cell line for human x human hybridoma production.
abstract: The potential of a new HAT-sensitive human lymphoblastoid cell line TK6 TG(r).P1. as a fusion partner was assessed, by comparison with the established human parental cell line UC729.6. Both of these cell lines were fused with the peripheral blood mononuclear cells of a patient with B-chronic lymphocytic leukaemia. The hybridomas generated in these fusion experiments were analysed by the fluorescence activated cell sorter and karyotyping. An anti-idiotype ELISA assay detected the presence of the patient's characteristic idiotype bearing immunoglobulin in the supernatant of a number of the hybridoma cell lines generated in both fusions.
authors.affiliation: Division of Biologics, Centre for Applied Microbiology, PHLS, Porton Down, Wiltshire, United Kingdom.
authors.forename: S A
authors.initials: SA
authors.lastname: Clark
authors.forename: S E
authors.initials: SE
authors.lastname: James
authors.forename: M
authors.initials: M
authors.lastname: Fitchett