title: Positive effects of treatment of donor cells with aphidicolin on the preimplantation development of somatic cell nuclear transfer embryos in Chinese Bama mini-pig (Sus Scrofa).
abstract: To optimize somatic cell nuclear transfer (SCNT) procedures in mini-pigs, the present study was designed to examine the effects of donor cell types and aphidicolin (APC) treatment on in vitro development of reconstructed embryos. Oviduct epithelial cells (OEC), ear fibroblast cells (EFC) and cumulus cells (CC) derived from mini-pigs were treated with serum starvation only or serum starvation followed by treatment of 0.1 µg/mL APC. The reconstructed embryos were cultured for 7 days to evaluate their developmental competency. Cleavage and blastocyst formation rates of reconstructed embryos derived from the OEC by APC treatment were significantly higher than the serum starvation (61.82% vs. 56.25%, 24.55% vs. 17.86%; P < 0.05). The cleavage rate from the EFC was significantly increased by APC treatment compared to serum starvation only (63.36% vs. 57.01%; P < 0.05). In the ooctyes with the CC, the reconstructed embryos could yield high blastocyst formation rate by APC treatment (29.63%; P < 0.05). In the presence of APC, CC gave rise to the highest cleavage and blastocyst formation rates among the three cell types. Therefore, our results suggest that treatment of CC with serum starvation plus APC prior to nuclear transfer is more suitable in SCNT of mini-pigs.
authors.affiliation: Animal Husbandry and Veterinary Research Institute, Shanghai Academy of Agricultural Sciences Shanghai, China.
authors.forename: Ting-Yu
authors.initials: TY
authors.lastname: Zhang
authors.forename: Jian-Jun
authors.initials: JJ
authors.lastname: Dai
authors.forename: Cai-Feng
authors.initials: CF
authors.lastname: Wu
authors.forename: Xiao-Long
authors.initials: XL
authors.lastname: Gu
authors.forename: Liang
authors.initials: L
authors.lastname: Liu
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Wu
authors.forename: Yi-Ni
authors.initials: YN
authors.lastname: Xie
authors.forename: Bin
authors.initials: B
authors.lastname: Wu
authors.forename: Hui-Lan
authors.initials: HL
authors.lastname: Chen
authors.forename: Yao
authors.initials: Y
authors.lastname: Li
authors.forename: Xue-Jin
authors.initials: XJ
authors.lastname: Chen
authors.forename: De-Fu
authors.initials: DF
authors.lastname: Zhang
mesh_terms: D000818:Animals; D016590:Aphidicolin / Q000494:pharmacology*; D001755:Blastocyst; D002478:Cells, Cultured; D054885:Cumulus Cells / Q000166:cytology*; D046149:Embryo Culture Techniques; D004622:Embryo, Mammalian / Q000196:embryology*; D047108:Embryonic Development / Q000187:drug effects*; D004847:Epithelial Cells; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D053652:Nuclear Transfer Techniques; D010057:Oviducts / Q000166:cytology; D044967:Serum; D034421:Sus scrofa / Q000196:embryology*
chemical_list: D016590:Aphidicolin