title: Detection and Molecular Characterization of Cryptosporidium species in Recreational Waters of Chaharmahal va Bakhtiyari Province of Iran using nested-PCR-RFLP.
abstract: BACKGROUND
  The aim of this study was to detect and characterize Cryptosporidium spp. in water samples collected from recreational ponds of Chaharmahal va Bakhtiyari Province of Iran.
  
  
  METHODS
  Thirty water samples were collected from November 2009 to May 2010. Each sample contained 10 liters of water. We used the SSU rRNA-based PCR-RFLP technique.
  
  
  RESULTS
  Out of thirty samples examined, 6 (20%) were positive for different Cryptosporidium spp. Restriction pattern analysis showed that C. parvum has been the most prevalent genotype, followed by C. hominis and C. canis, respectively. In this area, the higher prevalence of C. parvum compared with other genotypes is consistent with the distribution of cattle.
  
  
  CONCLUSIONS
  Farm animals, particularly cattle are the main source of cryptosporidial contamination for recreational waters in this area.
authors.affiliation: Department of Parasitology, Mycology and Entomology, School of Medicines, Shahr-e-kord University of Medical Science, Shahr-e-kord, Iran.
authors.forename: K
authors.initials: K
authors.lastname: Manouchehri Naeini
authors.forename: M
authors.initials: M
authors.lastname: Asadi
authors.forename: M
authors.initials: M
authors.lastname: Hashemzade Chaleshtori