title: Mechanosensing in T lymphocyte activation.
abstract: Mechanical forces play an increasingly recognized role in modulating cell function. This report demonstrates mechanosensing by T cells, using polyacrylamide gels presenting ligands to CD3 and CD28. Naive CD4 T cells exhibited stronger activation, as measured by attachment and secretion of IL-2, with increasing substrate elastic modulus over the range of 10-200 kPa. By presenting these ligands on different surfaces, this report further demonstrates that mechanosensing is more strongly associated with CD3 rather than CD28 signaling. Finally, phospho-specific staining for Zap70 and Src family kinase proteins suggests that sensing of substrate rigidity occurs at least in part by processes downstream of T-cell receptor activation. The ability of T cells to quantitatively respond to substrate rigidly provides an intriguing new model for mechanobiology.
authors.affiliation: Department of Biomedical Engineering, Columbia University, New York, New York, USA.
authors.forename: Edward
authors.initials: E
authors.lastname: Judokusumo
authors.forename: Erdem
authors.initials: E
authors.lastname: Tabdanov
authors.forename: Sudha
authors.initials: S
authors.lastname: Kumari
authors.forename: Michael L
authors.initials: ML
authors.lastname: Dustin
authors.forename: Lance C
authors.initials: LC
authors.lastname: Kam
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D000818:Animals; D000906:Antibodies / Q000276:immunology; D000938:Antigen-Presenting Cells / Q000166:cytology / Q000378:metabolism; D001696:Biomechanical Phenomena; D018106:CD28 Antigens / Q000276:immunology / Q000378:metabolism; D017252:CD3 Complex / Q000276:immunology / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology* / Q000276:immunology / Q000378:metabolism*; D055595:Mechanical Phenomena; D040542:Mechanotransduction, Cellular; D051379:Mice; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D000906:Antibodies; D018106:CD28 Antigens; D017252:CD3 Complex; D000099166:Polyacrylamides