title: Accuracy of correction in modal sensorless adaptive optics.
abstract: We investigate theoretically and experimentally the parameters governing the accuracy of correction in modal sensorless adaptive optics for microscopy. On the example of two-photon fluorescence imaging, we show that using a suitable number of measurements, precise correction can be obtained for up to 2 radians rms aberrations without optimising the aberration modes used for correction. We also investigate the number of photons required for accurate correction when signal acquisition is shot-noise limited. We show that only 10(4) to 10(5) photons are required for complete correction so that the correction process can be implemented with limited extra-illumination and associated photoperturbation. Finally, we provide guidelines for implementing an optimal correction algorithm depending on the experimental conditions.
authors.affiliation: Laboratory for Optics and Biosciences, Ecole Polytechnique, CNRS, INSERM, 91128 Palaiseau, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Facomprez
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Beaurepaire
authors.forename: Delphine
authors.initials: D
authors.lastname: Débarre
mesh_terms: D000465:Algorithms; D016477:Artifacts; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods*; D007909:Lenses; D008853:Microscopy / Q000295:instrumentation*; D012098:Reproduction; D012680:Sensitivity and Specificity; D014159:Transducers