title: A process for high-efficiency isoflavone deglycosylation using Bacillus subtilis natto NTU-18.
abstract: In order to produce isoflavone aglycosides effectively, a process of isoflavone hydrolysis by Bacillus subtilis natto NTU-18 (BCRC 80390) was established. This process integrates the three stages for the production of isoflavone aglycosides in one single fermenter, including the growth of B. subtilis natto, production of β-glucosidase, deglycosylation of fed isoflavone glycosides. After 8 h of batch culture of B. subtilis natto NTU-18 in 2 L of soy medium, a total of 3 L of soy isoflavone glucoside solution containing 3.0 mg/mL of daidzin and 1.0 mg/mL of genistin was fed continuously over 34 h. The percentage deglycosylation of daidzin and genistin was 97.7% and 94.6%, respectively. The concentration of daidzein and genistein in the broth reached 1,066.8 μg/mL (4.2 mM) and 351 μg/mL (1.3 mM), respectively, and no residual daidzin or genistin was detected. The productivity of the bioconversion of daidzein and genistein over the 42 h of culture was 25.6 mg/L/h and 8.5 mg/L/h, respectively. This showed that this is an efficient bioconversion process for selective estrogen receptor modulator production.
authors.affiliation: Department of Biochemical Science and Technology, National Taiwan University, Taipei, Taiwan, Republic of China.
authors.forename: Lun-Cheng
authors.initials: LC
authors.lastname: Kuo
authors.forename: Ren-Yu
authors.initials: RY
authors.lastname: Wu
authors.forename: Kung-Ta
authors.initials: KT
authors.lastname: Lee
mesh_terms: D001412:Bacillus subtilis / Q000378:metabolism*; D001711:Biotransformation; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D007529:Isoflavones / Q000378:metabolism*; D013997:Time Factors
chemical_list: D003470:Culture Media; D007529:Isoflavones; C040641:genistin; C013908:daidzin