title: Triplet pregnancy in a cynomolgus monkey (Macaca fascicularis) after double embryo transfer.
abstract: At our research center, cynomolgus monkeys (Macaca fascicularis) are bred by mating or intracytoplasmic sperm injection (ICSI) and embryo transfer. We typically transfer 2 embryos, because the pregnancy rate is better than that for single embryo transfer. In the case we present  here, 2 embryos that had been frozen and thawed after ICSI were transplanted into a recipient female macaque, and a multiple pregnancy (3 fetuses) was confirmed. All 3 fetuses were miscarried between days 81 and 85 of pregnancy. One fetus, which was wrapped in the amnion, was expelled along  with its own placenta and one other. Because the other placenta had 2 umbilical arteries, 2 fetuses may have shared it. Therefore, we believe this pregnancy was a case of triplets, including a set of twins from an embryo that divided after transfer.
authors.affiliation: Research Center for Animal Life Science, Shiga University of Medical Science, Otsu, Shiga, Japan.
authors.forename: Juri
authors.initials: J
authors.lastname: Morichika
authors.forename: Chizuru
authors.initials: C
authors.lastname: Iwatani
authors.forename: Hideaki
authors.initials: H
authors.lastname: Tsuchiya
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Nakamura
authors.forename: Tadashi
authors.initials: T
authors.lastname: Sankai
authors.forename: Ryuzo
authors.initials: R
authors.lastname: Torii
mesh_terms: D000034:Abortion, Veterinary; D000818:Animals; D000830:Animals, Laboratory; D004624:Embryo Transfer / Q000662:veterinary*; D005260:Female; D008252:Macaca fascicularis / Q000502:physiology*; D011247:Pregnancy; D011272:Pregnancy, Multiple / Q000502:physiology*; D020554:Sperm Injections, Intracytoplasmic