title: Precise H1N1 swine influenza detection using stuffer-free multiplex ligation-dependent probe amplification in conformation-sensitive capillary electrophoresis.
abstract: The H1N1 influenza virus has spread worldwide to become pandemic. Here, we developed a new method to discriminate various types of influenza A, including H1N1, using stuffer-free multiplex ligation-dependent probe amplification based on a conformation-sensitive separation method, namely capillary electrophoresis-single-strand conformation polymorphism. Unlike conventional methods, our approach precisely detects five relevant gene markers permitting confirmation of infection.
authors.affiliation: School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang, Gyeongbuk 790-784, Republic of Korea.
authors.forename: Boram
authors.initials: B
authors.lastname: Chung
authors.forename: Gi Won
authors.initials: GW
authors.lastname: Shin
authors.forename: Hee Sung
authors.initials: HS
authors.lastname: Hwang
authors.forename: Yeun-Jun
authors.initials: YJ
authors.lastname: Chung
authors.forename: Seung Won
authors.initials: SW
authors.lastname: Jung
authors.forename: Gyoo Yeol
authors.initials: GY
authors.lastname: Jung
mesh_terms: D000818:Animals; D015342:DNA Probes / Q000378:metabolism; D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics; D019075:Electrophoresis, Capillary / Q000379:methods*; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000302:isolation & purification*; D057230:Limit of Detection; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D009690:Nucleic Acid Conformation; D009976:Orthomyxoviridae Infections / Q000821:virology*; D018807:Polymorphism, Single-Stranded Conformational; D013552:Swine / Q000821:virology*
chemical_list: D015342:DNA Probes; D004279:DNA, Viral