title: Determination of propofol using high performance liquid chromatography in whole blood with fluorescence detection.
abstract: High-performance liquid chromatography method for the determination of propofol has been developed and validated. Following a liquid extraction using ethyl acetate and hexane, samples were separated by reverse-phase high-performance liquid chromatography on an XBridge C(18) column and quantified using fluorescence detection at an excitation of 276 nm and an emission of 310 nm. The mobile phase was a mixture of water (pH 4.0) and acetonitrile, with a flow rate of 1.5 mL/min. The standard curve ranged from 5-2000 ng/mL. Intra- and inter-assay variability for propofol was less than 10%, and the average recovery was greater than 95%. This assay is suitable for use in pharmacokinetic studies.
authors.affiliation: University of Tennessee, Departments of Biomedical and Diagnostic Sciences, College of Veterinary Medicine, Knoxville, TN 37996, USA. jyarbro2@utk.edu
authors.forename: Jason
authors.initials: J
authors.lastname: Yarbrough
authors.forename: Ralph
authors.initials: R
authors.lastname: Harvey
authors.forename: Sherry
authors.initials: S
authors.lastname: Cox
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004285:Dogs; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D016014:Linear Models; D000432:Methanol / Q000737:chemistry; D015742:Propofol / Q000097:blood*; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D000097:Acetonitriles; D000432:Methanol; D015742:Propofol; C032159:acetonitrile