title: A novel synthesis and detection method for cap-associated adenosine modifications in mouse mRNA.
abstract: A method is described for the detection of certain nucleotide modifications adjacent to the 5' 7-methyl guanosine cap of mRNAs from individual genes. The method quantitatively measures the relative abundance of 2'-O-methyl and N(6),2'-O-dimethyladenosine, two of the most common modifications. In order to identify and quantitatify the amounts of N(6),2'-O-dimethyladenosine, a novel method for the synthesis of modified adenosine phosphoramidites was developed. This method is a one step synthesis and the product can directly be used for the production of N(6),2'-O-dimethyladenosine containing RNA oligonucleotides. The nature of the cap-adjacent nucleotides were shown to be characteristic for mRNAs from individual genes transcribed in liver and testis.
authors.affiliation: School of Chemistry, The University of Nottingham, University Park, Nottingham, NG7 2RD, UK.
authors.forename: Susanne
authors.initials: S
authors.lastname: Kruse
authors.forename: Silin
authors.initials: S
authors.lastname: Zhong
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Bodi
authors.forename: James
authors.initials: J
authors.lastname: Button
authors.forename: Marcos J C
authors.initials: MJ
authors.lastname: Alcocer
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Hayes
authors.forename: Rupert
authors.initials: R
authors.lastname: Fray
mesh_terms: D000241:Adenosine / Q000737:chemistry; D000818:Animals; D001483:Base Sequence; D002855:Chromatography, Thin Layer; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D015394:Molecular Structure; D012314:RNA Cap Analogs / Q000138:chemical synthesis* / Q000737:chemistry / Q000235:genetics; D012333:RNA, Messenger / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D013737:Testis / Q000378:metabolism
chemical_list: D012314:RNA Cap Analogs; D012333:RNA, Messenger; D000241:Adenosine