title: Serum levels and H/L gene polymorphism of mannose-binding lectin in primary open angle glaucoma.
abstract: OBJECTIVE
  To analyze the serum levels and H/L gene polymorphisms of mannose-binding lectin-2 (MBL-2) in primary open angle glaucoma (POAG) cases and control subjects to investigate whether MBL-2 has a possible role in the development and pathogenesis of POAG.
  
  
  METHODS
  In 45 POAG cases and age and sex-matched 45 healthy controls, Elisa Kit was used to determine serum levels of MBL-2. The genomic DNA of patient and control groups was extracted from whole blood using High Pure PCR template preparation kit. Genotyping of MBL-2 polymorphisms were detected by using a MBL-2 mutation detection kit in real-time PCR. Chi-square or Fisher's Exact Tests were used to evaluate the distribution of MBL-2 H/L genotypes among patients and control subjects. Associations between the H/L genotype and POAG risk were analyzed by using binary logistic regression. The serum MBL-2 levels of both groups were compared with Independent Sample t-test.
  
  
  RESULTS
  Mean MBL-2 serum levels in the patient group (21.30 ± 4.97 µg/mL) was significantly higher than the control group (17.48 ± 3.66 µg/mL), (p < 0.001). The distribution of alleles in the patient group was 28.9% for LL, 44.4% for HL, 26.7% for HH and in controls was 33.3% for LL, 37.8% for HL, 28.3% for HH. According to genotype ratios, the two groups were not different from each other.
  
  
  CONCLUSIONS
  Our findings may suggest an association between high serum MBL-2 levels and POAG, but H/L gene polymorphism of MBL-2 seems not to be associated with POAG.
authors.affiliation: Mersin State Hospital, Ophthalmology Clinic, Mersin, Turkey.
authors.forename: Ozer
authors.initials: O
authors.lastname: Dursun
authors.forename: Ayça
authors.initials: A
authors.lastname: Yilmaz
authors.forename: Lokman
authors.initials: L
authors.lastname: Ayaz
authors.forename: Lülüfer
authors.initials: L
authors.lastname: Tamer
mesh_terms: D004252:DNA Mutational Analysis; D015342:DNA Probes / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005838:Genotype; D060005:Genotyping Techniques; D005902:Glaucoma, Open-Angle / Q000097:blood / Q000235:genetics*; D006801:Humans; D008297:Male; D037601:Mannose-Binding Lectin / Q000097:blood* / Q000235:genetics*; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
chemical_list: D015342:DNA Probes; C445619:MBL2 protein, human; D037601:Mannose-Binding Lectin