title: MR imaging of human pancreatic cancer xenograft labeled with superparamagnetic iron oxide in nude mice.
abstract: The aim of this work was to investigate the MRI findings on tumor xenografts induced in nude mice by the inoculation of human pancreatic cancer cells labeled with superparamagnetic iron oxide (SPIO), and to monitor the kinetics of SPIO distribution in tumor xenografts. The labeled cancer cells were subcutaneously inoculated into 11 nude mice to induce tumor xenograft. The unlabeled cancer cells served as a control inoculated into nine nude mice. MR imaging was performed with a 1.5 T MR scanner for the tumor xenograft at the first, second and third week after the inoculation. We found that the tumor xenograft was induced in 100% nude mice on MR imaging for both groups in the first week after the inoculation. In the SPIO group, the tumors showed homogeneous hypointensity on T₁ - and T₂ -weighted and FIESTA images 1 week after inoculation. Two and 3 weeks after inoculation, the center of the tumors was still hypointense on all the above sequences. The tumor periphery was isointense on T₁ -weighted, and hyperintense on T₂ -weighted and FIESTA images. The tumors in control group were homogeneously hypointense or isointense on T₁ -weighted, and hyperintense on T₂ -weighted and FIESTA images in the first, second and third week after the inoculation. The size and signal-to-noise ratio of the tumor center in the SPIO group had decreased subsequent to the inoculation in all T₁ - and T₂ -weighted images and FIESTA. Our results showed the human pancreatic cancer cells labeled with SPIO can induce tumor xenograft in nude mice and MRI can monitor the kinetics of SPIO distribution in tumor xenografts.
authors.affiliation: Sichuan Key Laboratory of Medical Imaging, Department of Radiology, Affiliated Hospital of North Sichuan Medical College, Sichuan 637007, China.
authors.forename: Chao Ying
authors.initials: CY
authors.lastname: Wu
authors.forename: Yu
authors.initials: Y
authors.lastname: Pu
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
authors.forename: Yang
authors.initials: Y
authors.lastname: Shao
authors.forename: Qing Song
authors.initials: QS
authors.lastname: Ma
authors.forename: Xiao Ming
authors.initials: XM
authors.lastname: Zhang
mesh_terms: D000818:Animals; D021441:Carcinoma, Pancreatic Ductal / Q000473:pathology*; D045744:Cell Line, Tumor / Q000637:transplantation; D058948:Cell Tracking / Q000379:methods*; D003287:Contrast Media; D003342:Corpus Striatum / Q000473:pathology; D003911:Dextrans / Q000493:pharmacokinetics; D006801:Humans; D007091:Image Processing, Computer-Assisted; D021621:Imaging, Three-Dimensional / Q000379:methods*; D058085:Iron Compounds / Q000032:analysis / Q000493:pharmacokinetics; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles; D051379:Mice; D008819:Mice, Nude; D010087:Oxides / Q000032:analysis / Q000493:pharmacokinetics; D010190:Pancreatic Neoplasms / Q000473:pathology*; D014018:Tissue Distribution; D014183:Transplantation, Heterologous
chemical_list: D003287:Contrast Media; D003911:Dextrans; D058085:Iron Compounds; D058185:Magnetite Nanoparticles; D010087:Oxides; C065507:ferumoxides