title: Behavior of almond oil bodies during in vitro gastric and intestinal digestion.
abstract: An aqueous suspension of almond oil bodies (about 10% lipids) was prepared and subjected to in vitro gastric (with pepsin) and intestinal (with bile salts and pancreatin) digestion, simulating fasting conditions. The physicochemical and structural changes of the almond oil body emulsion were examined. The almond oil body emulsion behaved similarly to a protein-stabilized emulsion, with flocculation of the oil bodies occurring under gastric conditions. Proteins, peptides, and phospholipids covered the surface of the oil bodies throughout gastric digestion. Under intestinal conditions, bile salts displaced the interfacial peptides and phospholipids, and disrupted the flocs. Gastric pepsinolysis of almond proteins was a prerequisite for their digestion in the duodenum. The oil body membrane had a negative impact on the efficiency of gastric digestion, and long chain fatty acids, the main lipolytic products, accumulated at the surface of the oil bodies and therefore limited the activity of pancreatic lipase.
authors.affiliation: Riddet Institute, Massey University, Private Bag 11 222, Palmerston North, 4442, New Zealand. S.Gallier@massey.ac.nz
authors.forename: Sophie
authors.initials: S
authors.lastname: Gallier
authors.forename: Harjinder
authors.initials: H
authors.lastname: Singh
mesh_terms: D001647:Bile Acids and Salts / Q000737:chemistry; D055598:Chemical Phenomena; D004063:Digestion; D004655:Emulsions; D005230:Fatty Acids, Nonesterified / Q000737:chemistry / Q000378:metabolism; D005524:Food Technology; D005750:Gastric Juice / Q000201:enzymology / Q000378:metabolism*; D007700:Kinetics; D008066:Lipolysis; D010189:Pancreatic Juice / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism*; D010316:Particle Size; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism; D010938:Plant Oils / Q000737:chemistry / Q000378:metabolism*; D028321:Plant Preparations / Q000737:chemistry / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism; D059748:Proteolysis; D027861:Prunus / Q000737:chemistry; D012639:Seeds / Q000737:chemistry; D013499:Surface Properties; D013535:Suspensions
chemical_list: D001647:Bile Acids and Salts; D004655:Emulsions; D005230:Fatty Acids, Nonesterified; D010743:Phospholipids; D010938:Plant Oils; D028321:Plant Preparations; D010940:Plant Proteins; D013535:Suspensions; C068582:almond oil