title: Optimization of a bacterial consortium for nitrobenzene degradation.
abstract: Three bacterial strains, Arthrobacter sp. NB1, Serratia sp. NB2 and Stenotrophomonas sp. NB3, were isolated from contaminated sludge by using nitrobenzene as a sole source of carbon and nitrogen. It was observed that all three strains could degrade nitrobenzene at 400 mg/L initial concentration and mixed-cultivation of these strains could enhance the degradation of nitrobenzene compared with mono-cultivation. Mixture design was used for adjusting the proportions of each strain and the optimal ratio of inoculation size was NB1:NB2:NB3 = 4:4:5, where the nitrobenzene degradation percentage was two times higher than for by the single strain. The results of Plackett-Burman design indicated that Mg(2+), Ca(2+), Fe(2+), Zn(2+) and Mn(2+) had a positive effect on the degradation of nitrobenzene, while Cu(2+) and Co(2+) had a negative effect on it.
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou, China.
authors.forename: D F
authors.initials: DF
authors.lastname: Jin
authors.forename: H
authors.initials: H
authors.lastname: Hu
authors.forename: D F
authors.initials: DF
authors.lastname: Liu
authors.forename: H T
authors.initials: HT
authors.lastname: Ding
authors.forename: X M
authors.initials: XM
authors.lastname: Jia
authors.forename: Y H
authors.initials: YH
authors.lastname: Zhao
mesh_terms: D000704:Analysis of Variance; D001173:Arthrobacter / Q000302:isolation & purification / Q000378:metabolism; D001419:Bacteria / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D008954:Models, Biological; D009578:Nitrobenzenes / Q000378:metabolism*; D012044:Regression Analysis; D015203:Reproducibility of Results; D012705:Serratia / Q000302:isolation & purification / Q000378:metabolism; D020588:Stenotrophomonas / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D009578:Nitrobenzenes; C036077:nitrobenzene