title: [Antitumor activity of polysaccharides extracted from two wild amanitas].
abstract: OBJECTIVE
  To investigate the anti-tumor activity of two wild Amanita strains, and to determine the inhibition ratio of their effective chemical components on tumor cells in vitro.
  
  
  METHODS
  Two wild Amanita strains collected in Sichuan province were identified through morphology and molecular methods. The chemical components from the fruit body of the two strains were extracted with petroleum ether, chloroform, methanol and water. The anti-tumor activity of the extracts were tested on the cancer cells of MGC and Lovo strains. The polysaccharides were isolated with hot water and tested for its anti-tumor activity in vitro.
  
  
  RESULTS
  The two wild Amanitas were identified as Amanita subjunquillea and Amanita pantherina. The water-soluble extracts from the Amanitas had anti-tumor effect, with polysaccharide as one of the main effective components. The IC50 value of the polysaccharide isolated from M1 was 3.790 mg/mL for the MGC cells.
  
  
  CONCLUSIONS
  Polysaccharides isolated from the two wild Amanitas have considerable anti-tumor activity against MGC cells in vitro.
authors.affiliation: Key Laboratory of Micro-resourse and Micro-technique, College of Life Science, Sichuan University, Chengdu 610065, China.
authors.forename: Yuan-lin
authors.initials: YL
authors.lastname: Tang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Luo
authors.forename: Wen
authors.initials: W
authors.lastname: Ding
authors.forename: Xiang
authors.initials: X
authors.lastname: Ding
authors.forename: Zhi-rong
authors.initials: ZR
authors.lastname: Yang
mesh_terms: D000545:Amanita / Q000737:chemistry* / Q000145:classification; D000970:Antineoplastic Agents / Q000302:isolation & purification* / Q000494:pharmacology; D045744:Cell Line, Tumor; D006801:Humans; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D011134:Polysaccharides