title: Techniques for the in vivo assessment of cardio-renal function in zebrafish (Danio rerio) larvae.
abstract: Zebrafish, a well-established vertebrate model, offer unique advantages for assessing renal function and physiology. Assays determining renal glomerular function based on cardiovascular erythrocyte flow and reduction of injected FITC-inulin were developed, each validated using the nephrotoxin gentamicin. Bland–Atlman analysis showed a strong association between measurements of the rate of inulin excretion and that of fluorescent reduction from the arterial vasculature. Reduced renal clearance of inulin, resulting from gentamicin or NaCl loading, was concurrent with reduced erythrocyte velocity, and yolk sac and pericardium oedema. These techniques, assessing pronephric function, highlight the potential for in vivo physiological study in this genetically tractable model.
authors.affiliation: University/BHF Centre for Cardiovascular Science, The Queen's Medical Research Institute, College of Medicine and Veterinary Medicine, 47 Little France Crescent, Edinburgh EH16 4TJ, UK. srider@staffmail.ed.ac.uk
authors.forename: Sebastien A
authors.initials: SA
authors.lastname: Rider
authors.forename: Carl S
authors.initials: CS
authors.lastname: Tucker
authors.forename: Jorge
authors.initials: J
authors.lastname: del-Pozo
authors.forename: Kirsten N
authors.initials: KN
authors.lastname: Rose
authors.forename: Calum A
authors.initials: CA
authors.lastname: MacRae
authors.forename: Matthew A
authors.initials: MA
authors.lastname: Bailey
authors.forename: John J
authors.initials: JJ
authors.lastname: Mullins
mesh_terms: D000818:Animals; D002320:Cardiovascular Physiological Phenomena; D006321:Heart / Q000502:physiology*; D007444:Inulin / Q000378:metabolism; D007677:Kidney Function Tests; D007678:Kidney Glomerulus / Q000502:physiology*; D007814:Larva; D023421:Models, Animal; D014553:Urinary Tract Physiological Phenomena; D015027:Zebrafish / Q000502:physiology*
chemical_list: D007444:Inulin