title: Use of the wound-inducible NtQPT2 promoter from Nicotiana                      tabacum for production of a plant-made vaccine.
abstract: The wound-inducible quinolinate phosphoribosyl transferase promoter from Nicotiana                         tabacum (NtQPT2) was assessed for its capacity to produce B-subunit of the heat-labile toxin (LTB) from enterotoxigenic Escherichia                         coli in transgenic plant tissues. Comparisons were made with the widely used and constitutive Cauliflower Mosaic Virus 35S (CaMV35S) promoter. The NtQPT2 promoter produced somewhat lower average concentrations of LTB protein per unit weight of hairy root tissue but allowed better growth thereby producing similar or higher overall average yields of LTB per culture batch. Transgenic tobacco plants containing the NtQPT2-LTB construct contained LTB protein in roots but not leaves. Moreover, wounding NtQPT2-LTB transgenic plants, by removal of apices, resulted in an approximate 500% increase in LTB levels in roots when analysed several days later. CaMV35S-LTB transgenic plants contained LTB protein in leaves and roots but wounding made no difference to their LTB content.
authors.affiliation: School of Biological Sciences, Monash University, Clayton, VIC, Australia. Giorgio.deguzman@monash.edu.au
authors.forename: Giorgio
authors.initials: G
authors.lastname: De Guzman
authors.forename: Amanda M
authors.initials: AM
authors.lastname: Walmsley
authors.forename: Diane E
authors.initials: DE
authors.lastname: Webster
authors.forename: John D
authors.initials: JD
authors.lastname: Hamill
mesh_terms: D001427:Bacterial Toxins / Q000096:biosynthesis* / Q000235:genetics; D004768:Enterotoxins / Q000096:biosynthesis* / Q000235:genetics; D029968:Escherichia coli Proteins / Q000096:biosynthesis* / Q000235:genetics; D010430:Pentosyltransferases / Q000235:genetics*; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism*; D014612:Vaccines / Q000096:biosynthesis* / Q000235:genetics; D000098553:Nicotinate-Nucleotide Diphosphorylase (Carboxylating)
chemical_list: D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D014612:Vaccines; C027538:heat-labile enterotoxin, E coli; D010430:Pentosyltransferases; D000098553:Nicotinate-Nucleotide Diphosphorylase (Carboxylating)