title: The effect of the D1-C785T polymorphism in the type 1 iodothyronine deiodinase gene on the circulating thyroid hormone levels in Romanian women with preeclampsia. Association with the degree of severity and pregnancy outcome of preeclampsia.
abstract: OBJECTIVE
  To investigate the biochemical and genetic thyroid status in women with preeclampsia by the determination of serum FT3 and FT4 levels in association with D1-C785T genotypes.
  
  
  METHODS
  We genotyped using PCR-RFLP methods 50 women with preeclampsia and 50 normotensive pregnant women.
  
  
  RESULTS
  FT3 levels (pg/ml, 2.63 ± 0.56 vs. 2.91 ± 1.41) were low, and FT4 levels (ng/dl, 1.11 ± 0.3 vs. 0.88 ± 0.14) were high in women with preeclampsia compared to normal pregnant women. The association with severe preeclampsia was stronger for the homozygous T/T genotype (OR 6.57, p = 0.029). Women with preeclampsia with the D1-T785 mutated allele had lower FT3 levels (pg/ml, 2.31 ± 0.81 vs. 3.04 ± 0.39, p < 0.001), higher FT4 levels (ng/dl, 1.32 ± 0.87 vs. 0.84 ± 0.24, p = 0.009) than women with preeclampsia with the D1-C/C genotype. Significant decrease in serum FT3 levels in positive women with severe preeclampsia compared to women negative for this genetic variation (pg/ml, 1.59 ± 0.74 vs. 2.77 ± 0.23, p = 0.003) was observed. Women with severe preeclampsia, positive for the mutated T785 allele, delivered at a significantly lower gestational age (31.75 ± 3.69 vs. 38.66 ± 3.21 weeks, p = 0.035) neonates with a lower birth weight (1861.11 ± 869.9 vs. 3500 ± 424.26 g, p = 0.023) compared to women negative for the same allele.
  
  
  CONCLUSIONS
  Thyroid hormone levels and the D1-C785T polymorphism, alone or in combination, correlate with the severity of preeclampsia. The D1-C785T polymorphism influences the outcome of pregnancy in severe preeclampsia.
authors.affiliation: Department of Medical Biochemistry, Iuliu Hatieganu University of Medicine and Pharmacy Cluj-Napoca, Romania. luciamariaprocopciuc@yahoo.com
authors.forename: L M
authors.initials: LM
authors.lastname: Procopciuc
authors.forename: G M
authors.initials: GM
authors.lastname: Hazi
authors.forename: G
authors.initials: G
authors.lastname: Caracostea
authors.forename: G
authors.initials: G
authors.lastname: Nemeti
authors.forename: I
authors.initials: I
authors.lastname: Olteanu
authors.forename: G H
authors.initials: GH
authors.lastname: Dragatoiu
authors.forename: F
authors.initials: F
authors.lastname: Stamatian
mesh_terms: D000328:Adult; D005260:Female; D006801:Humans; D007453:Iodide Peroxidase / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011225:Pre-Eclampsia / Q000097:blood* / Q000235:genetics; D011247:Pregnancy; D011256:Pregnancy Outcome; D012383:Romania; D013960:Thyroid Function Tests; D013974:Thyroxine / Q000097:blood*; D014284:Triiodothyronine / Q000097:blood*; D055815:Young Adult
chemical_list: D014284:Triiodothyronine; C109812:iodothyronine deiodinase type I; D007453:Iodide Peroxidase; D013974:Thyroxine