title: Nano-structured arrays for multiplex analyses and Lab-on-a-Chip applications.
abstract: Nanospheres lithographic (NSL) method has been used to fabricate nano-structured arrays (NAs) of hexagonally close-packed gold (Au) using polystyrene beads [PS, diameter ∼300 nm] as mask. The developed NA was incorporated with a customized and cheap microfluidics system to demonstrate its applicability as an alternative easy and efficient platform for multiplex analysis and Lab-on-a-Chip applications. The chip functionality was demonstrated with horseradish peroxidase (HRP) and anti-HRP antibody as model for recognition system. The enzyme-linked immunosorbent assay (ELISA) performed on fabricated protein biochip had a detection limit 100 pg/mL for HRP. The antibody chip was also checked for the shelf-life and it was found that these chips could be stored for 50 days when stored at 4°C without any significant loss of activity. Therefore, NAs based protein biochip with the correct microfluidics could find huge potential application in diagnostics and biosensing technology.
authors.affiliation: National Centre for Sensor Research, Dublin City University, Glasnevin, Dublin 9, Ireland. Chandra.dixit2@mail.dcu.ie
authors.forename: Chandra K
authors.initials: CK
authors.lastname: Dixit
authors.forename: Ajeet
authors.initials: A
authors.lastname: Kaushik
mesh_terms: D055910:Antibodies, Immobilized / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006046:Gold; D006735:Horseradish Peroxidase / Q000276:immunology; D056656:Lab-On-A-Chip Devices; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D049329:Nanostructures; D011137:Polystyrenes / Q000737:chemistry; D040081:Protein Array Analysis / Q000295:instrumentation*; D012834:Silver
chemical_list: D055910:Antibodies, Immobilized; D011137:Polystyrenes; D012834:Silver; D006046:Gold; D006735:Horseradish Peroxidase