title: Differential contribution of dendritic cell CD1d to NKT cell-enhanced humoral immunity and CD8+ T cell activation.
abstract: CD1d-restricted type I NKT cells provide help for specific antibody production. B cells, which have captured and presented a T-dependent, antigen-derived peptide on MHC class II and CD1d-binding glycolipid α-GC on CD1d, respectively, activate Th and NKT cells to elicit B cell help. However, the role of the DC CD1d in humoral immunity remains unknown. We therefore constructed mixed bone marrow chimeras containing CD1d-expressing, DTR-transgenic DCs and CD1d(+) or CD1d(-) nontransgenic DCs. Following DT-mediated DC ablation and immunization, we observed that the primary and secondary antibody responses were equivalent in the presence of CD1d(+) and CD1d(-) DCs. In contrast, a total ablation of DCs delayed the primary antibody response. Further experiments revealed that depletion of CD1d(+) DCs blocked in vivo expansion of antigen-specific cytotoxic (CD8(+)) T lymphocytes. These results provide a clear demonstration that although CD1d expression on DCs is essential for NKT-enhanced CD8(+) T cell expansion, it is dispensable for specific antibody production.
authors.affiliation: University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA. sunil-joshi@ouhsc.edu
authors.forename: Sunil K
authors.initials: SK
authors.lastname: Joshi
authors.forename: Gillian A
authors.initials: GA
authors.lastname: Lang
authors.forename: T Scott
authors.initials: TS
authors.lastname: Devera
authors.forename: Amy M
authors.initials: AM
authors.lastname: Johnson
authors.forename: Susan
authors.initials: S
authors.lastname: Kovats
authors.forename: Mark L
authors.initials: ML
authors.lastname: Lang
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D055610:Antigens, CD1d / Q000276:immunology* / Q000378:metabolism*; D015153:Blotting, Western; D001853:Bone Marrow / Q000276:immunology / Q000378:metabolism; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D007114:Immunization; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D055611:Natural Killer T-Cells / Q000276:immunology* / Q000378:metabolism
chemical_list: D055610:Antigens, CD1d; D008070:Lipopolysaccharides