title: [Association between mannose-binding-lectin gene and type 2 diabetic patients in Chinese population living in the northern areas of China].
abstract: OBJECTIVE
  To investigate whether the mannose-binding-lectin 2 (MBL2) gene was associated with type 2 diabetes in the populations living the northern part of China.
  
  
  METHODS
  The study involved 318 type 2 diabetic patients and 448 normoglycemic controls. The variances of rs1800450, rs1800451 and rs11003125 were determined by the Multiplex SNaPshot method. Fasting blood-glucose, triglyceride and total cholesterol were also measured. All of these results were analyzed by logistic regression method. Linkage disequilibrium and Haplotype measures were computed in all samples using Haploview.
  
  
  RESULTS
  There seemed no mutation on rs1800451 while the rs1800450 and rs11003125 polymorphism was consistent with Hardy-Weinberg expectations in both the case and the control groups. Genotypes and allele frequencies of rs1800450 as well as rs11003125 were observed (P = 0.006, P = 0.003) and (P = 0.010, P = 0.004), respectively. Data from logistic regression analysis revealed that factors as overweight, abdominal obesity, hypercholesterolemia, GG genotype frequencies of Exon1 rs1800450 polymorphism as well as (GC + CC) genotype frequencies of rs11003125 polymorphism in MBL2 conferred increased risks for type 2 diabetes. Haplotype analyses of the two SNPs (rs1800450, rs11003125) revealed similar effects as compared with the single SNP associations. Only haplotype constructed from GC alleles conferred increased trends for type 2 diabetes (OR = 2.21, 95%CI: 1.47 - 3.33, P = 0.000).
  
  
  CONCLUSIONS
  Our result suggested that the Exon1 rs1800450 polymorphism and promoter region rs11003125 polymorphism in MBL2 gene were both associated with type 2 diabetes in the Chinese population living in the northern areas of China. The G allele of rs1800450 and C allele of rs11003125 might be the risk factors of type 2 diabetes.
authors.affiliation: School of Public Health, Shandong University, Jinan 250012, China.
authors.forename: Na-na
authors.initials: NN
authors.lastname: Zhang
authors.forename: Ai-xia
authors.initials: AX
authors.lastname: Ma
authors.forename: Ping
authors.initials: P
authors.lastname: Cheng
authors.forename: Mao-qiang
authors.initials: MQ
authors.lastname: Zhuang
authors.forename: Fei-fei
authors.initials: FF
authors.lastname: Cao
authors.forename: Xing-dong
authors.initials: XD
authors.lastname: Chen
authors.forename: Shu-yuan
authors.initials: SY
authors.lastname: Li
authors.forename: Ming
authors.initials: M
authors.lastname: Lü
mesh_terms: D000368:Aged; D044466:Asian People / Q000235:genetics; D002681:China / Q000453:epidemiology; D003924:Diabetes Mellitus, Type 2 / Q000453:epidemiology / Q000208:ethnology / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D037601:Mannose-Binding Lectin / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide
chemical_list: C445619:MBL2 protein, human; D037601:Mannose-Binding Lectin