title: Crystallography on a chip.
abstract: A new chip-based crystal-mounting approach for rapid room-temperature data collection from numerous crystals is described. This work was motivated by the recent development of X-ray free-electron lasers. These novel sources deliver very intense femtosecond X-ray pulses that promise to yield high-resolution diffraction data of nanocrystals before their destruction by radiation damage. Thus, the concept of `diffraction before destruction' requires rapid replenishment of the sample for each exposure. The chip promotes the self-assembly of an array of protein crystals on a surface. Rough features on the surface cause the crystals to adopt random orientations, allowing efficient sampling of reciprocal space.
authors.affiliation: Max Planck Research Group for Structural Dynamics, Center for Free-Electron Laser Science, Department of Physics, University of Hamburg, Notkestrasse 85, 22607 Hamburg, Germany.
authors.forename: Arash
authors.initials: A
authors.lastname: Zarrine-Afsar
authors.forename: Thomas R M
authors.initials: TR
authors.lastname: Barends
authors.forename: Christina
authors.initials: C
authors.lastname: Müller
authors.forename: Martin R
authors.initials: MR
authors.lastname: Fuchs
authors.forename: Lukas
authors.initials: L
authors.lastname: Lomb
authors.forename: Ilme
authors.initials: I
authors.lastname: Schlichting
authors.forename: R J Dwayne
authors.initials: RJ
authors.lastname: Miller
mesh_terms: D018360:Crystallography, X-Ray / Q000295:instrumentation* / Q000379:methods; D007834:Lasers; D053758:Nanoparticles / Q000737:chemistry; D011506:Proteins / Q000737:chemistry
chemical_list: D011506:Proteins