title: Osteogenic effect of high-frequency acceleration on alveolar bone.
abstract: Mechanical stimulation contributes to the health of alveolar bone, but no therapy using the osteogenic effects of these stimuli to increase alveolar bone formation has been developed. We propose that the application of high-frequency acceleration to teeth in the absence of significant loading is osteogenic. Sprague-Dawley rats were divided among control, sham, and experimental groups. The experimental group underwent localized accelerations at different frequencies for 5 min/day on the occlusal surface of the maxillary right first molar at a very low magnitude of loading (4 µε). Sham rats received a similar load in the absence of acceleration or frequency. The alveolar bone of the maxilla was evaluated by microcomputed tomography (µCT), histology, fluorescence microscopy, scanning electron microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR imaging), and RT-PCR for osteogenic genes. Results demonstrate that application of high-frequency acceleration significantly increased alveolar bone formation. These effects were not restricted to the area of application, and loading could be replaced by frequency and acceleration. These studies propose a simple mechanical therapy that may play a significant role in alveolar bone formation and maintenance.
authors.affiliation: Consortium for Translational Orthodontic Research, New York University College of Dentistry, New York, NY 10010, USA.
authors.forename: M
authors.initials: M
authors.lastname: Alikhani
authors.forename: E
authors.initials: E
authors.lastname: Khoo
authors.forename: B
authors.initials: B
authors.lastname: Alyami
authors.forename: M
authors.initials: M
authors.lastname: Raptis
authors.forename: J M
authors.initials: JM
authors.lastname: Salgueiro
authors.forename: S M
authors.initials: SM
authors.lastname: Oliveira
authors.forename: A
authors.initials: A
authors.lastname: Boskey
authors.forename: C C
authors.initials: CC
authors.lastname: Teixeira
mesh_terms: D000054:Acceleration; D000539:Alveolar Process / Q000502:physiology*; D000818:Animals; D001696:Biomechanical Phenomena; D015519:Bone Density / Q000502:physiology; D002113:Calcification, Physiologic / Q000502:physiology; D002254:Carbonates / Q000032:analysis; D003094:Collagen / Q000648:ultrastructure; D021621:Imaging, Three-Dimensional / Q000379:methods; D008297:Male; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D008963:Molar / Q000502:physiology; D008964:Molar, Third / Q000502:physiology; D009991:Oscillometry; D010012:Osteogenesis / Q000502:physiology*; D010812:Physical Stimulation; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017550:Spectroscopy, Fourier Transform Infrared; D013314:Stress, Mechanical; D014732:Vibration / Q000627:therapeutic use*; D055114:X-Ray Microtomography / Q000379:methods
chemical_list: D002254:Carbonates; D003094:Collagen