title: Use of CPY and its derivatives to study protein quality control in various cell compartments.
abstract: Mutated derivatives of carboxypeptidase yscY (CPY) are potent substrates to study protein quality control and protein degradation in different cell compartments in yeast. Depending on the subcellular compartment of interest, the design of the model substrate used has to be adapted. Here, we describe the derivatives of CPY used in genetic screens based on a sensitive growth test in order to identify new components of the protein quality control systems in different degradation pathways.
authors.affiliation: Institute of Biochemistry, University of Stuttgart, Stuttgart, Germany.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Stolz
authors.forename: Dieter H
authors.initials: DH
authors.lastname: Wolf
mesh_terms: D043402:Cathepsin A / Q000378:metabolism*; D009154:Mutation; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D017510:Protein Folding; D059748:Proteolysis; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D054875:Ubiquitination
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D043402:Cathepsin A; C499283:PRC1 protein, S cerevisiae; D046988:Proteasome Endopeptidase Complex; C061553:ATP dependent 26S protease