title: Liquid chromatography/tandem mass spectrometry method for quantification of trans-stilbene glycoside in rat plasma and its pharmacokinetic application.
abstract: A rapid and sensitive liquid chromatography/tandem mass spectrometry (LC-MS/MS) method was developed and validated for the quantification of trans-stilbene glycoside (SG) in rat plasma. As trans-SG can be rapidly isomerized under light exposure, trans-SG plasma samples were prepared in the dark and assayed immediately. Trans-SG and internal standard were extracted by protein precipitation. Chromatographic separation was achieved on a C(18) column with a gradient elution program. The detection of analytes was performed by negative ion via multiple reaction monitoring mode. The precursor-to-product ions of m/z 405.1 → 242.9 for trans-SG and m/z 389.1 → 226.9 for polydatin (internal standard) were monitored. No interference of endogenous components was observed for any plasma samples that we studied.The method was linear over the concentration range of 1.0-1000.0 ng/mL with a good correlation coefficient. The lower limit of quantification was 1.0 ng/mL for trans-SG. The intra and inter-batch accuracy for trans-SG in stable rat plasma samples ranged from 93.3 to 102.7% and the variation was less than 8.1%. The extraction recoveries of trans-SG in rat plasma were from 102.8 to 112.4% and the matrix effects were also acceptable. This method was successfully applied to pharmacokinetic study of trans-SG in rats after intravenous administration.
authors.affiliation: College of Pharmacy, Nanjing University of Traditional Chinese Medicine, Nanjing, 210046, China.
authors.forename: Jiankun
authors.initials: J
authors.lastname: Wang
authors.forename: Xuanxuan
authors.initials: X
authors.lastname: Zhu
authors.forename: Ying
authors.initials: Y
authors.lastname: Peng
authors.forename: Weibin
authors.initials: W
authors.lastname: Zha
authors.forename: Dong
authors.initials: D
authors.lastname: Feng
authors.forename: Yinci
authors.initials: Y
authors.lastname: Zhu
authors.forename: Ping
authors.initials: P
authors.lastname: Wan
authors.forename: Huanhuan
authors.initials: H
authors.lastname: Qi
authors.forename: Jichao
authors.initials: J
authors.lastname: He
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.forename: Jianguo
authors.initials: J
authors.lastname: Sun
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D005960:Glucosides / Q000097:blood / Q000737:chemistry; D006027:Glycosides / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D016014:Linear Models; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013267:Stilbenes / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D005960:Glucosides; D006027:Glycosides; D013267:Stilbenes; C058229:polydatin