title: Effects of N1-methylnicotinamide on oxidative and glycooxidative stress markers in rats with streptozotocin-induced diabetes mellitus.
abstract: OBJECTIVE
  This study was focused on the monitoring how the anti-inflammatory substance, N(1)-methylnicotinamide (MNA), could influence oxidation and glycooxidation stress markers in rats under conditions of streptozotocin (STZ)-induced diabetes mellitus.
  
  
  METHODS
  Diabetes mellitus was induced in 60 male Wistar rats by intraperitoneal injection of STZ and after 7 days diabetic animals were allocated to five groups according to the dose of MNA administered for 7 weeks. The degree of DNA damage in lymphocytes, as well as advanced glycation endproducts (AGEs), protein carbonyls, lipid peroxides, and total antioxidant capacity (TEAC) in plasma were measured.
  
  
  RESULTS
  Glycation damage to proteins (represented by AGEs level) was significantly increased in all diabetic groups compared to untreated non-diabetic animals. MNA did not affect TEAC of plasma in any group of diabetic rats. Supplementation of diabetic rats with MNA at the dose of 200 mg/kg resulted in decreased protein carbonyls (from 0.0818±0.0091 to 0.0558±0.0044 nmol/mg proteins; P<0.05, n=15) and DNA oxidation, reflected by the levels of 8-oxoG (0.6302±0.085 vs. 0.9213±0.108 8-oxoG/10(6) G; P<0.05, n=15), compared to untreated diabetic animals.
  
  
  CONCLUSIONS
  Our results demonstrated that MNA at suitable concentrations could influence oxidative modifications of proteins and DNA.
authors.affiliation: Faculty of Medicine, Institute of Medical Chemistry, Biochemistry and Clinical Biochemistry, Comenius University, and Pharmaco-biochemical Laboratory, Third Internal Clinics of Faculty Hospital, Bratislava, Slovakia. zuzana.orszaghova@fmed.uniba.sk
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Országhová
authors.forename: Ol'ga
authors.initials: O
authors.lastname: Uličná
authors.forename: Anna
authors.initials: A
authors.lastname: Liptáková
authors.forename: Ingrid
authors.initials: I
authors.lastname: Zitňanová
authors.forename: Jana
authors.initials: J
authors.lastname: Muchová
authors.forename: Cezary
authors.initials: C
authors.lastname: Watala
authors.forename: Zdeňka
authors.initials: Z
authors.lastname: Duračková
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis; D004249:DNA Damage; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism* / Q000473:pathology; D004353:Drug Evaluation, Preclinical; D017127:Glycation End Products, Advanced / Q000032:analysis; D015227:Lipid Peroxidation / Q000187:drug effects; D008054:Lipid Peroxides / Q000097:blood; D008214:Lymphocytes / Q000378:metabolism / Q000473:pathology; D008297:Male; D009536:Niacinamide / Q000031:analogs & derivatives* / Q000494:pharmacology; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D050050:Protein Carbonylation / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D017127:Glycation End Products, Advanced; D008054:Lipid Peroxides; D009536:Niacinamide; D013311:Streptozocin; C024058:N(1)-methylnicotinamide