title: Isolation of NEDDylated proteins in human cells.
abstract: Identification of substrates for post-translational modification with ubiquitin and ubiquitin-like molecules is a key in our understanding of the role of this modification in the regulation of biological processes. The ubiquitin-like molecule NEDD8 plays a vital role in cell growth, viability, and development. In this -chapter, we describe a method for efficient isolation and detection of NEDDylated proteins in human cells.
authors.affiliation: Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee, Scotland, UK.
authors.forename: Orsolya
authors.initials: O
authors.lastname: Leidecker
authors.forename: Dimitris P
authors.initials: DP
authors.lastname: Xirodimas
mesh_terms: D015153:Blotting, Western / Q000379:methods; D002460:Cell Line; D006801:Humans; D000075687:NEDD8 Protein; D011499:Protein Processing, Post-Translational; D025801:Ubiquitin / Q000378:metabolism*; D014452:Ubiquitins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000075687:NEDD8 Protein; C082860:NEDD8 protein, human; D025801:Ubiquitin; D014452:Ubiquitins