title: Cytotoxicity evaluation of 63s bioactive glass and bone-derived hydroxyapatite particles using human bone-marrow stem cells.
abstract: BACKGROUND
  In recent years, bioceramics have been favored by biomaterials scientists and researchers. Due to their special and distinctive features, bioactive glass and hydroxyapatite possess a higher place among different types of bioceramics.
  
  
  METHODS
  In this study, the effect of 63S bioactive glass and bone-derived hydroxyapatite particles on the proliferation of human bone-marrow stem cells (hMSCs) was investigated. Bioactive glass particles were made via sol-gel method and hydroxyapatite was obtained from bovine bone. The particle size and morphology were investigated by scanning electron microscope (SEM). Then the in vitro cytotoxicity of particles was evaluated using MTT assay. SEM showed that bioactive glass particles were in the nanoscale range and had tendency towards agglomeration. It was also confirmed that the hydroxyapatite particles were agglomerations of crystals cca 50-500 nm across.
  
  
  RESULTS
  The results of MTT assay confirmed the viability and proliferation of hMSCs in contact with bioactive glass and bone-derived HA particles. The fabricated particles in combination with stem cells were shown to hold promising potential for further applications in tissue engineering and regenerative medicine.
authors.affiliation: Biomaterials Group, Department of Materials Engineering, Isfahan University of Technology, Isfahan, Iran. alidm14@ma.iut.ac.ir
authors.forename: Ali
authors.initials: A
authors.lastname: Doostmohammadi
authors.forename: Ahmad
authors.initials: A
authors.lastname: Monshi
authors.forename: Rasoul
authors.initials: R
authors.lastname: Salehi
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Fathi
authors.forename: Ehsan
authors.initials: E
authors.lastname: Seyedjafari
authors.forename: Abbas
authors.initials: A
authors.lastname: Shafiee
authors.forename: Masoud
authors.initials: M
authors.lastname: Soleimani
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D001854:Bone Marrow Cells / Q000502:physiology*; D001842:Bone and Bones / Q000737:chemistry*; D002417:Cattle; D049109:Cell Proliferation; D005898:Glass; D006801:Humans; D006882:Hydroxyapatites / Q000302:isolation & purification / Q000633:toxicity*; D066298:In Vitro Techniques; D008855:Microscopy, Electron, Scanning; D010316:Particle Size; D013234:Stem Cells / Q000502:physiology*; D018675:Toxicity Tests
chemical_list: D001672:Biocompatible Materials; D006882:Hydroxyapatites; C570103:bioactive glass 63S