title: Local serotonin mediates cyclic strain-induced phenotype transformation, matrix degradation, and glycosaminoglycan synthesis in cultured sheep mitral valves.
abstract: This study addressed the following questions: 1) Does cyclic tensile strain induce protein expression patterns consistent with myxomatous degeneration in mitral valves? 2) Does cyclic strain induce local serotonin synthesis in mitral valves? 3) Are cyclic strain-induced myxomatous protein expression patterns in mitral valves dependent on local serotonin? Cultured sheep mitral valve leaflets were subjected to 0, 10, 20, and 30% cyclic strain for 24 and 72 h. Protein levels of activated myofibroblast phenotype markers, α-smooth muscle actin (α-SMA) and nonmuscle embryonic myosin (SMemb); matrix catabolic enzymes, matrix metalloprotease (MMP) 1 and 13, and cathepsin K; and sulfated glycosaminoglycan (GAG) content in mitral valves increased with increased cyclic strain. Serotonin was present in the serum-free media of cultured mitral valves and concentrations increased with cyclic strain. Expression of the serotonin synthetic enzyme tryptophan hydroxylase 1 (TPH1) increased in strained mitral valves. Pharmacologic inhibition of the serotonin 2B/2C receptor or TPH1 diminished expression of phenotype markers (α-SMA and SMemb) and matrix catabolic enzyme (MMP1, MMP13, and cathepsin K) expression in 10- and 30%-strained mitral valves. These results provide first evidence that mitral valves synthesize serotonin locally. The results further demonstrate that tensile loading modulates local serotonin synthesis, expression of effector proteins associated with mitral valve degeneration, and GAG synthesis. Inhibition of serotonin diminishes strain-mediated protein expression patterns. These findings implicate serotonin and tensile loading in mitral degeneration, functionally link the pathogeneses of serotoninergic (carcinoid, drug-induced) and degenerative mitral valve disease, and have therapeutic implications.
authors.affiliation: Department of Clinical Sciences, Colorado State University, Fort Collins, CO 80523-1678, USA. Carla.Lacerda@colostate.edu
authors.forename: Carla M R
authors.initials: CM
authors.lastname: Lacerda
authors.forename: John
authors.initials: J
authors.lastname: Kisiday
authors.forename: Brennan
authors.initials: B
authors.lastname: Johnson
authors.forename: E Christopher
authors.initials: EC
authors.lastname: Orton
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D001696:Biomechanical Phenomena / Q000502:physiology; D056657:Cathepsin K / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism*; D006025:Glycosaminoglycans / Q000378:metabolism*; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D053509:Matrix Metalloproteinase 13 / Q000378:metabolism; D008943:Mitral Valve / Q000378:metabolism* / Q000473:pathology*; D009218:Myosins / Q000378:metabolism; D009924:Organ Culture Techniques; D010641:Phenotype; D012701:Serotonin / Q000378:metabolism*; D012756:Sheep; D013718:Tensile Strength / Q000502:physiology; D014365:Tryptophan Hydroxylase / Q000378:metabolism
chemical_list: D000199:Actins; D006025:Glycosaminoglycans; D012701:Serotonin; D014365:Tryptophan Hydroxylase; D056657:Cathepsin K; D053509:Matrix Metalloproteinase 13; D020781:Matrix Metalloproteinase 1; D009218:Myosins