title: Growth, differentiation, and migration of osteoblasts on transparent Ni doped TiO2 thin films deposited on borosilicate glass.
abstract: A simple and cost effective dip coating method was used to deposit thin films of amorphous (AM) or anatase (AN) titanium dioxide (TiO(2)) on borosilicate glass substrates, either with or without prior doping of TiO(2) with nickel (Ni) cations by a specially designed sol gel technique. The objective of the study was to compare the physicochemical and biological properties of these films and assess their use in orthopedic implants or for in vitro cell biological studies. Analytical techniques such as XRD and XPS, in combination with ATR-FTIR and SEM revealed that only AN films, prepared by controlled heating up to 450°C, irrespective of prior doping with Ni, contained significant crystalline structures of variable morphologies. This observation could be linked to the carbon and oxygen contents and the availability of functional groups in the films. Cell biological studies revealed that Ni doping of TiO(2) in both AM and AN films improved the adhesion, spreading, proliferation, differentiation, and migration of MC3T3 cells. Our studies provide a new approach to prepare optically transparent metal surfaces, with tunable physicochemical properties, which could be suitable for eliciting optimal osteoinductive cell responses and permit the detailed in vitro cell biological studies of osteoblasts.
authors.affiliation: CSIR-Centre for Cellular and Molecular Biology, Hyderabad, India. Marshal@ccmb.res.in
authors.forename: Marshal
authors.initials: M
authors.lastname: Dhayal
authors.forename: Renu
authors.initials: R
authors.lastname: Kapoor
authors.forename: Pavana Goury
authors.initials: PG
authors.lastname: Sistla
authors.forename: Chander
authors.initials: C
authors.lastname: Kant
authors.forename: Ravi Ranjan
authors.initials: RR
authors.lastname: Pandey
authors.lastname: Govind
authors.forename: Krishan Kumar
authors.initials: KK
authors.lastname: Saini
authors.forename: Gopal
authors.initials: G
authors.lastname: Pande
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002113:Calcification, Physiologic / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005898:Glass; D051379:Mice; D009532:Nickel / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects* / Q000201:enzymology; D056951:Photoelectron Spectroscopy; D017640:Silicates / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared; D014025:Titanium / Q000494:pharmacology*; D014961:X-Ray Diffraction
chemical_list: D017640:Silicates; C009495:titanium dioxide; D009532:Nickel; D014025:Titanium; D000469:Alkaline Phosphatase