title: Molecular analysis of transgenic melon plants showing virus resistance conferred by direct repeat of movement gene of Cucumber green mottle mosaic virus.
abstract: Cucumber green mottle mosaic virus (CGMMV) is a major limiting factor in the production of melon plants worldwide. For effective control of this virus using the transgenic approach, the direct repeat of the movement protein gene of CGMMV was used for transforming melon plants by Agrobacterium tumefaciens. PCR and Southern blot analyses of T₃ confirmed that they carried the transgene. Northern blot analysis with total RNA showed that transgene transcript RNA as well as siRNA was observed in all plants tested. Separate leaves or individual plants were inoculated with CGMMV and subjected to ELISA and RNA blot analysis using the coat protein gene probe of the virus. Compared to nontransgenic control, these plants were shown to have high virus resistance. Furthermore, cytosine of the transgene DNA in the plants was methylated. Thus, these results reveal that the transgenic lines were highly resistant to the virus through RNA silencing. Key message High virus resistance was obtained in transgenic melon plants with direct repeat of movement protein gene of Cucumber green mottle mosaic tobamovirus through RNA silencing.
authors.affiliation: Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama 790-8566, Japan.
authors.forename: Emran Md
authors.initials: EM
authors.lastname: Ali
authors.forename: Ali
authors.initials: A
authors.lastname: Emran
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Tabei
authors.forename: Kappei
authors.initials: K
authors.lastname: Kobayashi
authors.forename: Naoto
authors.initials: N
authors.lastname: Yamaoka
authors.forename: Masamichi
authors.initials: M
authors.lastname: Nishiguchi
mesh_terms: D015152:Blotting, Northern; D015139:Blotting, Southern; D017799:Cucumovirus / Q000235:genetics*; D019662:Cucurbitaceae / Q000235:genetics* / Q000821:virology*; D019175:DNA Methylation / Q000235:genetics; D018744:DNA, Plant / Q000378:metabolism; D060467:Disease Resistance / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D005814:Genes, Viral / Q000235:genetics*; D010935:Plant Diseases / Q000821:virology*; D053830:Plant Viral Movement Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D019076:Transgenes / Q000235:genetics
chemical_list: D018744:DNA, Plant; D053830:Plant Viral Movement Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering