title: Regulated expressions of MMP-2, -9 by diterpenoids from Euphorbia formosana Hayata.
abstract: Two new abietane type diterpenoids, namely seco-helioscopinolide (1) and 3b,7b-dihydroxy-ent-abieta-8,13-diene-12,16-olide (2) were isolated from the aerial parts of Euphorbia formosana Hayata together with helioscopinolide A (3), helioscopinolide B (4), helioscopinolide C (5) and ent-(5b,8a,9b,10a,12a)-12-hydroxyatis-16-ene-3,14-dione (6). The structures of compounds 1-6 were elucidated by analyzing their spectroscopic data and comparison with the literature. Further biological tests by gelatin zymographic analysis revealed that 3-5 significantly up-regulated the expressions and activation of MMP-2 and -9 in human fibrosarcoma cell line HT1080.
authors.affiliation: Graduate Institute of Pharmacognosy, Taipei Medical University, 250 Wu Xin Street, Taipei 110, Taiwan.
authors.forename: Chia-Chun
authors.initials: CC
authors.lastname: Yu
authors.forename: Ching-Ruey
authors.initials: CR
authors.lastname: Hsieh
authors.forename: George
authors.initials: G
authors.lastname: Hsiao
authors.forename: Pi-Yu
authors.initials: PY
authors.lastname: Chen
authors.forename: Meng-Lun
authors.initials: ML
authors.lastname: Chang
authors.forename: Hwa-Wen
authors.initials: HW
authors.lastname: Yin
authors.forename: Tzong-Huei
authors.initials: TH
authors.lastname: Lee
authors.forename: Ching-Kuo
authors.initials: CK
authors.lastname: Lee
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D004224:Diterpenes / Q000737:chemistry* / Q000494:pharmacology*; D028481:Euphorbia / Q000737:chemistry*; D005354:Fibrosarcoma / Q000188:drug therapy / Q000201:enzymology; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis* / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis* / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes; D010936:Plant Extracts; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9