title: A graphene oxide-based fluorescent aptasensor for the turn-on detection of epithelial tumor marker mucin 1.
abstract: Mucin 1 (MUC1) which presents in epithelial malignancies, is a well-known tumor biomarker. In this paper, a highly sensitive and selective fluorescent aptasensor for Mucin 1 (MUC1) detection is constructed, utilizing graphene oxide (GO) as a quencher which can quench the fluorescence of single-stranded dye-labeled MUC1 specific aptamer. In the absence of MUC1, the adsorption of the dye-labeled aptamer on GO brings the dyes in close proximity to the GO surface resulting in high efficiency quenching of dye fluorescence. Therefore, the fluorescence of the designed aptasensor is completely quenched by GO, and the system shows very low background fluorescence. Conversely, and very importantly, upon the adding of MUC1, the quenched fluorescence is recovered significantly, and MUC1 can be detected in a wide range of 0.04-10 μM with a detection limit of 28 nM and good selectivity. Moreover, the results have also been verified for real sample application by testing 2% serum containing buffer solution spiked with a series of concentrations of MUC1.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Research Center for Nanobiology and Nanomedicine (MOE 985 Innovative Platform), Wuhan University, Wuhan, 430072, PR China.
authors.forename: Yue
authors.initials: Y
authors.lastname: He
authors.forename: Yi
authors.initials: Y
authors.lastname: Lin
authors.forename: Hongwu
authors.initials: H
authors.lastname: Tang
authors.forename: Daiwen
authors.initials: D
authors.lastname: Pang
mesh_terms: D052158:Aptamers, Peptide / Q000737:chemistry*; D014408:Biomarkers, Tumor / Q000097:blood*; D001774:Blood Chemical Analysis / Q000295:instrumentation; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D018396:Mucin-1 / Q000097:blood*; D049329:Nanostructures / Q000737:chemistry; D009375:Neoplasms, Glandular and Epithelial / Q000097:blood* / Q000175:diagnosis; D010087:Oxides / Q000737:chemistry; D010316:Particle Size; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*
chemical_list: D052158:Aptamers, Peptide; D014408:Biomarkers, Tumor; D018396:Mucin-1; D010087:Oxides; D006108:Graphite