title: Lycopene inhibits caspase-3 activity and reduces oxidative organ damage in a rat model of thermal injury.
abstract: Oxidative stress has been implicated in various pathological processes including burn induced multiple organ damage. This study investigated the effects of lycopene treatment against oxidative injury in rats with thermal trauma. Under ether anesthesia, shaved dorsum of the rats was exposed to 90°C bath for 10s to induce burn and treated either vehicle (olive oil) or lycopene (50mg/kg orally). Rats were decapitated 48 h after injury and the tissue samples from lung and kidney were taken for histological analysis and the determination of malondialdehyde (MDA) and glutathione (GSH) levels, myeloperoxidase (MPO), superoxide dismutase (SOD), catalase (CAT) and caspase-3 activities. Proinflammatory cytokines, TNF-α and IL-1β, were assayed in blood samples. Severe skin scald injury caused a significant decrease in GSH levels, SOD and CAT activities, and significant increases in MDA levels, MPO and caspase-3 activities of tissues. Similarly, plasma TNF-α and IL-1β were elevated in the burn group as compared to the control group. Lycopene treatment reversed all these biochemical indices. According to the findings of the present study, lycopene possesses antiinflammatory, antiapoptotic and antioxidant effects that prevents burn-induced oxidative damage in remote organs.
authors.affiliation: Marmara University, School of Pharmacy, Department of Biochemistry, Istanbul, Turkey.
authors.forename: Ozge
authors.initials: O
authors.lastname: Cevik
authors.forename: Rabia
authors.initials: R
authors.lastname: Oba
authors.forename: Cağlar
authors.initials: C
authors.lastname: Macit
authors.forename: Sule
authors.initials: S
authors.lastname: Cetinel
authors.forename: Ozlem Tuğçe Cilingir
authors.initials: OT
authors.lastname: Kaya
authors.forename: Emre
authors.initials: E
authors.lastname: Sener
authors.forename: Göksel
authors.initials: G
authors.lastname: Sener
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D002056:Burns / Q000150:complications / Q000188:drug therapy* / Q000378:metabolism; D002338:Carotenoids / Q000627:therapeutic use*; D053148:Caspase 3 / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D005978:Glutathione / Q000378:metabolism; D007674:Kidney Diseases / Q000378:metabolism / Q000517:prevention & control; D008171:Lung Diseases / Q000378:metabolism / Q000517:prevention & control; D000077276:Lycopene; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D016207:Cytokines; D002338:Carotenoids; D008315:Malondialdehyde; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase; D053148:Caspase 3; D005978:Glutathione; D000077276:Lycopene