title: The impact of the microbial load of genital mycoplasmas and gestational age on the intensity of intraamniotic inflammation.
abstract: OBJECTIVE
  The objective of the study was to determine whether the bacterial load of genital mycoplasmas and gestation age are related to intraamniotic inflammatory response using the amniotic fluid levels of 18 inflammatory mediators.
  
  
  METHODS
  A prospective study of 145 women with singleton pregnancies complicated by preterm prelabor rupture of membranes between 24(0/7) and 36(6/7) weeks was conducted. Amniotic fluid was obtained from all women by transabdominal amniocentesis. The amounts of genital mycoplasma deoxyribonucleic acid were determined using the threshold cycle value and relative and absolute quantification techniques. A panel of multiple proteins was analyzed simultaneously using multiplex technology.
  
  
  RESULTS
  Twenty-four women with the presence of genital mycoplasmas in amniotic fluid were included in the final analyses. The concentrations of 9 of the 18 evaluated proteins in the amniotic fluid correlated with bacterial load of genital mycoplasmas independent of the quantification technique used.
  
  
  CONCLUSIONS
  The intensity of intraamniotic inflammatory response to genital mycoplasmas decreased with gestational age.
authors.affiliation: Department of Obstetrics and Gynecology, Charles University in Prague, Prague, Czech Republic. kacermar@fnhk.cz
authors.forename: Marian
authors.initials: M
authors.lastname: Kacerovsky
authors.forename: Lenka
authors.initials: L
authors.lastname: Pliskova
authors.forename: Radka
authors.initials: R
authors.lastname: Bolehovska
authors.forename: Kristin
authors.initials: K
authors.lastname: Skogstrand
authors.forename: David M
authors.initials: DM
authors.lastname: Hougaard
authors.forename: Panagiotis
authors.initials: P
authors.lastname: Tsiartas
authors.forename: Bo
authors.initials: B
authors.lastname: Jacobsson
mesh_terms: D000328:Adult; D000649:Amniocentesis; D000653:Amniotic Fluid / Q000737:chemistry / Q000382:microbiology; D058491:Bacterial Load; D002821:Chorioamnionitis / Q000175:diagnosis / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D005322:Premature Rupture of Fetal Membranes / Q000382:microbiology; D005865:Gestational Age; D006801:Humans; D018836:Inflammation Mediators / Q000032:analysis; D009175:Mycoplasma Infections / Q000175:diagnosis*; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000175:diagnosis / Q000382:microbiology*; D011446:Prospective Studies; D012720:Severity of Illness Index; D055815:Young Adult
chemical_list: D004269:DNA, Bacterial; D018836:Inflammation Mediators