title: The need for speed.
abstract: One of the key enabling features of coherent Raman scattering (CRS) techniques is the dramatically improved imaging speed over conventional vibrational imaging methods. It is this enhanced imaging acquisition rate that has guided the field of vibrational microscopy into the territory of real-time imaging of live tissues. In this feature article, we review several aspects of fast vibrational imaging and discuss new applications made possible by the improved CRS imaging capabilities. In addition, we reflect on the current limitations of CRS microscopy and look ahead at several new developments towards real-time, hyperspectral vibrational imaging of biological tissues. (© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim).
authors.affiliation: Beckman Laser Institute, Department of Biomedical Engineering, University of California, Irvine, CA, USA.
authors.forename: Jeffrey L
authors.initials: JL
authors.lastname: Suhalim
authors.forename: John C
authors.initials: JC
authors.lastname: Boik
authors.forename: Bruce J
authors.initials: BJ
authors.lastname: Tromberg
authors.forename: Eric O
authors.initials: EO
authors.lastname: Potma
mesh_terms: D000818:Animals; D006801:Humans; D057054:Molecular Imaging / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013997:Time Factors; D014022:Tissue Survival