title: Identification of a common immune signature in murine and human systemic Salmonellosis.
abstract: Despite the importance of Salmonella infections in human and animal health, the target antigens of Salmonella-specific immunity remain poorly defined. We have previously shown evidence for antibody-mediating protection against invasive Salmonellosis in mice and African children. To generate an overview of antibody targeting in systemic Salmonellosis, a Salmonella proteomic array containing over 2,700 proteins was constructed and probed with immune sera from Salmonella-infected mice and humans. Analysis of multiple inbred mouse strains identified 117 antigens recognized by systemic antibody responses in murine Salmonellosis. Importantly, many of these antigens were independently identified as target antigens using sera from Malawian children with Salmonella bacteremia, validating the study of the murine model. Furthermore, vaccination with SseB, the most prominent antigenic target in Malawian children, provided mice with significant protection against Salmonella infection. Together, these data uncover an overlapping immune signature of disseminated Salmonellosis in mice and humans and provide a foundation for the generation of a protective subunit vaccine.
authors.affiliation: Center for Comparative Medicine, Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
authors.forename: Seung-Joo
authors.initials: SJ
authors.lastname: Lee
authors.forename: Li
authors.initials: L
authors.lastname: Liang
authors.forename: Silvia
authors.initials: S
authors.lastname: Juarez
authors.forename: Minelva R
authors.initials: MR
authors.lastname: Nanton
authors.forename: Esther N
authors.initials: EN
authors.lastname: Gondwe
authors.forename: Chisomo L
authors.initials: CL
authors.lastname: Msefula
authors.forename: Matthew A
authors.initials: MA
authors.lastname: Kayala
authors.forename: Francesca
authors.initials: F
authors.lastname: Necchi
authors.forename: Jennifer N
authors.initials: JN
authors.lastname: Heath
authors.forename: Peter
authors.initials: P
authors.lastname: Hart
authors.forename: Renée M
authors.initials: RM
authors.lastname: Tsolis
authors.forename: Robert S
authors.initials: RS
authors.lastname: Heyderman
authors.forename: Calman A
authors.initials: CA
authors.lastname: MacLennan
authors.forename: Philip L
authors.initials: PL
authors.lastname: Felgner
authors.forename: D Huw
authors.initials: DH
authors.lastname: Davies
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: McSorley
mesh_terms: D000818:Animals; D000917:Antibody Formation / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology; D001770:Blood Bactericidal Activity; D002648:Child; D002675:Child, Preschool; D003289:Convalescence; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008295:Malawi; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D040081:Protein Array Analysis; D015203:Reproducibility of Results; D012480:Salmonella Infections / Q000097:blood / Q000276:immunology*; D012481:Salmonella Infections, Animal / Q000276:immunology*; D014611:Vaccination; D014613:Vaccines, Attenuated / Q000276:immunology; D022223:Vaccines, Subunit / Q000276:immunology
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D014613:Vaccines, Attenuated; D022223:Vaccines, Subunit