title: Methods for registration of magnetic resonance images of ex vivo prostate specimens with histology.
abstract: OBJECTIVE
  To evaluate two methods of scanning and tissue processing to achieve accurate magnetic resonance (MR)-histologic correlation in human prostate specimens.
  
  
  METHODS
  Two prostates had acrylic paint markers injected to define the plane of imaging and serve as internal fiducials. Each was placed on a polycarbonate plane-finder device (PFD), which was adjusted to align the imaging and cutting planes. Three prostates were aligned by use of a plane finder key (PFK), a polycarbonate plate that locks the specimen in a cylindrical carrier. Markers were injected for registration analysis. Prostates were imaged, then sectioned. Imaging software was used to create registration maps of the MR and histology images. Measurements between control points were made and compared.
  
  
  RESULTS
  Accurate correlation was achieved between MR and histologic images. The mean displacement (MD) between the corresponding registration points using the PFD technique ranged from 1.11-1.38 mm for each section. The MD for all sections was 1.24 mm. The MD using the PFK technique ranged from 0.79-1.01 mm for each section, and the MD across all sections for the PFK was 0.92 mm.
  
  
  CONCLUSIONS
  We describe two methods that can achieve accurate, reproducible correlation between MR imaging and histologic sections in human prostatectomy specimens.
authors.affiliation: Department of Urology, Stanford University, Stanford, California, USA.
authors.forename: Simon Y
authors.initials: SY
authors.lastname: Kimm
authors.forename: Tatum V
authors.initials: TV
authors.lastname: Tarin
authors.forename: Jin Hyung
authors.initials: JH
authors.lastname: Lee
authors.forename: Bob
authors.initials: B
authors.lastname: Hu
authors.forename: Kristin
authors.initials: K
authors.lastname: Jensen
authors.forename: Dwight
authors.initials: D
authors.lastname: Nishimura
authors.forename: James D
authors.initials: JD
authors.lastname: Brooks
mesh_terms: D001706:Biopsy / Q000379:methods*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D057918:Fiducial Markers; D006801:Humans; D007089:Image Enhancement / Q000295:instrumentation / Q000379:methods; D066298:In Vitro Techniques; D008279:Magnetic Resonance Imaging / Q000295:instrumentation* / Q000379:methods*; D008297:Male; D011467:Prostate / Q000473:pathology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013382:Subtraction Technique / Q000295:instrumentation*