title: Preparation, crystallization and preliminary X-ray crystallographic analysis of OXA-23, a carbapenemase conferring widespread antibiotic resistance.
abstract: OXA-23, a class D carbapenemase that confers widespread antibiotic resistance hydrolyzes the beta-lactam rings of beta-lactam antibiotics, presenting an enormous challenge to infection control, particularly in the eradication of pathogenic bacteria such as Acinetobacter baumannii, one of six top-priority dangerous pathogens. Thus, the enzyme is a potential target for developing antimicrobial agents against pathogens producing carbapenemases. In this study, OXA-23 was purified and crystallized at 298 K and X-ray diffraction data from OXA-23 crystal were collected at 2.03 A resolution using synchrotron radiation. The crystal of OXA-23 belonged to space group P4(1) with unit cell parameters a = 82.47, b = 82.47 and c = 172.01 A. Analysis of the packing density showed that the asymmetric unit probably contained two molecules with a solvent content of 73.64%.
authors.affiliation: Drug Resistance Proteomics Laboratory, Department of Biological Sciences, Myongji University, 116 Myongjiro, Yongin, Gyeonggido 449-728, Republic of Korea.
authors.forename: Jung Hun
authors.initials: JH
authors.lastname: Lee
authors.forename: Seung Ghyu
authors.initials: SG
authors.lastname: Sohn
authors.forename: Ha Il
authors.initials: HI
authors.lastname: Jung
authors.forename: Young Jun
authors.initials: YJ
authors.lastname: An
authors.forename: Jae Jin
authors.initials: JJ
authors.lastname: Lee
authors.forename: Lin-Woo
authors.initials: LW
authors.lastname: Kang
authors.forename: Sang Hee
authors.initials: SH
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray; D004352:Drug Resistance, Microbial; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D001618:beta-Lactamases / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: C466679:beta-lactamase OXA-23; D001618:beta-Lactamases