title: Carboxymethylbetaine copolymer layer covalently fixed to a glass substrate.
abstract: A random copolymer of zwitterionic monomer, carboxymethylbetaine (CMB), and 3-methacryloyloxypropyl trimethoxysilane was prepared in ethanol using 2,2'-azobisisobutyronitrile as initiator. The incubation of ethanol solution of the copolymer with a glass plate gave a layer of the copolymer with a thickness of about 2-3 nm. The copolymer-modified glass substrate became highly hydrophilic upon immersion in water, and showed a resistance against non-specific adsorption of proteins, and the degree of resistance increased with the content of CMB residues in the copolymer and leveled off. The adhesion of various cells to the glass substrate was also strongly suppressed by the surface modification with the copolymer layer. Further introduction of PolyCMB graft chains on the surface of the layer enhanced the suppression of cell adhesion due to the steric hindrance for the cells to approach the layer. The usefulness of the combination of zwitterionic polymer layer and graft chains to afford anti-biofouling properties to a solid surface of metal oxides was shown.
authors.affiliation: Department of Applied Chemistry, Graduate School of Science and Engineering, University of Toyama, Toyama, Japan.
authors.forename: Hisatomo
authors.initials: H
authors.lastname: Suzuki
authors.forename: Lifu
authors.initials: L
authors.lastname: Li
authors.forename: Tadashi
authors.initials: T
authors.lastname: Nakaji-Hirabayashi
authors.forename: Hiromi
authors.initials: H
authors.lastname: Kitano
authors.forename: Kohji
authors.initials: K
authors.lastname: Ohno
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Matsuoka
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Saruwatari
mesh_terms: D016475:3T3 Cells; D000327:Adsorption / Q000187:drug effects; D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D000431:Ethanol / Q000737:chemistry; D005898:Glass / Q000737:chemistry; D057809:HEK293 Cells; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008689:Methacrylates / Q000737:chemistry*; D051379:Mice; D009570:Nitriles / Q000737:chemistry; D011485:Protein Binding / Q000187:drug effects; D011506:Proteins / Q000737:chemistry; D012821:Silanes / Q000737:chemistry*; D013499:Surface Properties; D050337:Trimethyl Ammonium Compounds / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D008689:Methacrylates; D009570:Nitriles; D011506:Proteins; D012821:Silanes; D050337:Trimethyl Ammonium Compounds; D014867:Water; C017492:methacryloxypropyltrimethoxysilane; D000431:Ethanol; C004526:azobis(isobutyronitrile)