title: CD25+ T cell depletion impairs murine squamous cell carcinoma development via modulation of antitumor immune responses.
abstract: Squamous cell carcinoma (SCC) constitutes a microenvironment that could modulate the antitumor immune response. Also, tumor-infiltrating lymphocytes are believed to play complex regulatory roles in antitumor immunity against SCC. The presence of regulatory T cells (Tregs) has been associated with the suppression of tumor-reactive T cells. However, the underlying mechanism for this T cell dysfunction is not clear. We used a multistage model of SCC to examine the role of Treg cells during tumor development. 7,12-dimethylbenz[a]-anthracene/phorbol 12-myristate 13-acetate treatment and systemic depletion of Treg cells using an anti-CD25 monoclonal antibody (PC61) resulted in a decrease in the number and incidence of papilloma. Furthermore, CD25 depletion increased the proportion of CD8(+) and CD4(+) T cells that were isolated from tumor lesions. The levels of interleukin (IL)-1β, IL-10, IL-12, IL-13, interferon-γ, transforming growth factor-β and tumor necrosis factor-α, but not IL-17, were increased in the tumor microenvironment after Treg depletion. Therefore, our results indicated involvement of CD25(+) T cells in SCC development and in the suppression of the inflammatory immune response.
authors.affiliation: Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, 17012-901 Bauru, SP, Brazil.
authors.forename: Rodrigo Nalio
authors.initials: RN
authors.lastname: Ramos
authors.forename: Carine Ervolino
authors.initials: CE
authors.lastname: Oliveira
authors.forename: Thais Helena
authors.initials: TH
authors.lastname: Gasparoto
authors.forename: Tatiana Salles de Souza
authors.initials: TS
authors.lastname: Malaspina
authors.forename: Eduardo Bertoli
authors.initials: EB
authors.lastname: Belai
authors.forename: Karen Angélica
authors.initials: KA
authors.lastname: Cavassani
authors.forename: Gustavo Pompermaier
authors.initials: GP
authors.lastname: Garlet
authors.forename: João Santana da
authors.initials: JS
authors.lastname: Silva
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Campanelli
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D016207:Cytokines / Q000378:metabolism; D005260:Female; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology*; D008212:Lymphocyte Depletion; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D016207:Cytokines; D053645:Interleukin-2 Receptor alpha Subunit