title: [The effect of interleukin-1beta and dexamethasone on keratinocyte growth factor expression level of cultured oral fibroblasts].
abstract: OBJECTIVE
  To detect the effects of human interleukin-1beta (IL-1beta) and Dexamethasone (DEX) on the expression level of keratinocyte growth factor (KGF) in cultured human fibroblasts of normal oral and oral lichen planus (OLP) mucosa.
  
  
  METHODS
  Three concentration gradients of IL-1beta and DEX were added to cultured fibroblasts of human normal oral and OLP mucosa respectively. 72 hours later, the supernatant was harvested for the detection of KGF concentration with enzyme linked immunosorbent assay (ELISA). Total RNA of the fibroblasts was extracted and reverse transcribed. The resulting cDNA was then amplified by polymerase chain reaction (PCR) to detect the KGF mRNA.
  
  
  RESULTS
  The results of the ELISA and PCR showed that the expression levels of KGF protein and mRNA were higher if the cells were treated with IL-1beta. However, the expression levels of KGF protein and mRNA were significantly reduced if the fibroblasts were treated with DEX.
  
  
  CONCLUSIONS
  IL-1beta can promote KGF expression levels of cultured normal oral and OLP fibroblasts, and it is concentration-dependent. While DEX can inhibit KGF expression of cultured normal oral and OLP fibroblasts, and it is also concentration-dependent.
authors.affiliation: Dept. of Periodontology, Yantai Stomatological Hospital, Yantai 264000, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Pan
authors.forename: Xiangmin
authors.initials: X
authors.lastname: Qi
authors.forename: Meng
authors.initials: M
authors.lastname: Yin
mesh_terms: D002478:Cells, Cultured; D003907:Dexamethasone; D051523:Fibroblast Growth Factor 7; D005347:Fibroblasts; D006801:Humans; D053583:Interleukin-1beta; D012333:RNA, Messenger
chemical_list: C496453:FGF7 protein, human; D053583:Interleukin-1beta; D012333:RNA, Messenger; D051523:Fibroblast Growth Factor 7; D003907:Dexamethasone