title: Fast volumetric imaging of ethanol metabolism in rat liver with hyperpolarized [1-(13) C]pyruvate.
abstract: Rapid volumetric imaging of hyperpolarized (13) C compounds allows the real-time measurement of metabolic activity and can be useful in distinguishing between normal and diseased tissues. This work extends a fast two-dimensional undersampled spiral MRSI sequence to provide volumetric coverage, acquiring a 16 × 16 × 12 matrix with a nominal isotropic resolution of 5 mm in 4.5 s. The rapid acquisition enables a high temporal resolution for dynamic imaging. This dynamic three-dimensional MRSI method was used to investigate hyperpolarized [1-(13) C]pyruvate metabolism modulated by the administration of ethanol in rat liver. A significant increase in the pyruvate to lactate conversion was observed in the liver as a result of the greater availability of NADH (nicotinamide adenine dinucleotide, reduced form) from ethanol metabolism.
authors.affiliation: SRI International, Neuroscience Program, Menlo Park, CA 94025, USA. sjosan@stanford.edu
authors.forename: Sonal
authors.initials: S
authors.lastname: Josan
authors.forename: Daniel
authors.initials: D
authors.lastname: Spielman
authors.forename: Yi-Fen
authors.initials: YF
authors.lastname: Yen
authors.forename: Ralph
authors.initials: R
authors.lastname: Hurd
authors.forename: Adolf
authors.initials: A
authors.lastname: Pfefferbaum
authors.forename: Dirk
authors.initials: D
authors.lastname: Mayer
mesh_terms: D000409:Alanine / Q000378:metabolism; D000818:Animals; D002247:Carbon Isotopes; D000431:Ethanol / Q000378:metabolism*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D007668:Kidney / Q000098:blood supply / Q000378:metabolism; D007700:Kinetics; D019344:Lactic Acid / Q000378:metabolism; D008099:Liver / Q000098:blood supply / Q000378:metabolism*; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011773:Pyruvates / Q000378:metabolism*; D051381:Rats; D013997:Time Factors
chemical_list: D002247:Carbon Isotopes; D011773:Pyruvates; D019344:Lactic Acid; D000431:Ethanol; D000409:Alanine