title: Autofluorescence--an important ancillary technique for the detection of Mycobacterium tuberculosis: revisited.
abstract: Tuberculosis is one of the oldest diseases known to affect humans and is caused by Mycobacterium tuberculosis. Lymph nodes are the most common site of extrapulmonary involvement. Tuberculous lymphadenitis can be presumptively diagnosed morphologically on fine-needle aspiration biopsy of lymph nodes. Additional tests like Ziehl-Neelsen (ZN) stain for detection of acid fast bacilli, fluorescence, and molecular markers are often employed for confirmation of diagnosis. However, culture is essential for obtaining a definitive diagnosis. Unfortunately, culture is time consuming and expensive. Newer investigative methods are required. The efficacy of autofluorescence in the diagnosis of tuberculous lymphadenitis was evaluated for this purpose. Fine-needle aspiration biopsies were collected from patients with a clinical diagnosis of tuberculous lymphadenitis. Cytomorphological examination, ZN staining, autofluorescence, and culture were performed on all specimens. ZN staining was positive in 37.5% (30/80) of cases, while autofluorescence was positive in 57.5% (46/80) of patients and was found to have 81.8% specificity, 95% sensitivity, and a positive predictive value of 82.6%. This technique allowed rapid and early diagnosis of tuberculous lymphadenitis in a number of patients, permitting early institution of appropriate therapy.
authors.affiliation: Department of Pathology, Moti Lal Nehru Medical College, Allahabad, India.
authors.forename: Parul
authors.initials: P
authors.lastname: Joshi
authors.forename: Mamta
authors.initials: M
authors.lastname: Singh
authors.forename: Anudita
authors.initials: A
authors.lastname: Bhargava
authors.forename: Mangal
authors.initials: M
authors.lastname: Singh
authors.forename: Ravi
authors.initials: R
authors.lastname: Mehrotra
mesh_terms: D000293:Adolescent; D000328:Adult; D044963:Biopsy, Fine-Needle; D002648:Child; D002675:Child, Preschool; D003470:Culture Media; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D061848:Optical Imaging / Q000379:methods*; D012680:Sensitivity and Specificity; D013194:Staining and Labeling; D014388:Tuberculosis, Lymph Node / Q000175:diagnosis*; D055815:Young Adult
chemical_list: D003470:Culture Media