title: Long-term effects of ovariectomy on osteoporosis and obesity in estrogen-receptor-β-deleted mice.
abstract: Untreated BERKO mice demonstrate few abnormalities in bone phenotype and recent ovariectomy has few effects on various bone characteristics in these mice. Long-term studies on the bone phenotype of intact and ovariectomized mice are unavailable. Using quantitative computed tomography  (qCT), we determined various parameters of the metaphysis of the tibia in sham-ovariectomized (intact) and ovariectomized BERKO and wildtype mice. Body weight and estrogen-regulated fat were also measured. Mice underwent surgery (ovariectomy or sham) at 3 mo of age, and qCT analysis was performed  every 2 to 4 mo until mice were 12 mo old. Ovariectomized wildtype mice gained body weight and their fat depot increased in size within 2 mo after ovariectomy. Obesity developed later in ovariectomized BERKO mice, which became significantly heavier than their wildtype counterparts. Ovariectomized  wildtype mice lost trabecular density more rapidly than did ovariectomized BERKO mice, which did not show similar loss in trabecular density until at least 7 mo after ovariectomy. At the latest studied time point (9 mo after surgery), cortical area was significantly larger in ovariectomized  BERKO mice than ovariectomized wildtype mice. The absence of ERβ in ovariectomized BERKO mice during the first 3 to 5 mo after ovariectomy had protective effects against obesity and trabecular rarification; this protective effect disappeared at later time points.
authors.affiliation: Department of Endocrinology, University Medical Center Göttingen, Göttingen, Germany. ufkendo@med.uni-goettingen.de
authors.forename: Dana
authors.initials: D
authors.lastname: Seidlova-Wuttke
authors.forename: Ba Tiep
authors.initials: BT
authors.lastname: Nguyen
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Wuttke
mesh_terms: D000367:Age Factors; D000704:Analysis of Variance; D000818:Animals; D001835:Body Weight; D016022:Case-Control Studies; D047629:Estrogen Receptor beta / Q000235:genetics*; D005260:Female; D051379:Mice; D018345:Mice, Knockout; D009765:Obesity / Q000209:etiology* / Q000473:pathology; D010024:Osteoporosis / Q000209:etiology* / Q000473:pathology; D010052:Ovariectomy; D013977:Tibia / Q000473:pathology*; D014057:Tomography, X-Ray Computed
chemical_list: D047629:Estrogen Receptor beta