title: Fungus-mediated biosynthesis and characterization of TiO₂ nanoparticles and their activity against pathogenic bacteria.
abstract: In the present study, the biosynthesis of TiO(2) nanoparticles (TiO(2) NPs) was achieved by a novel, biodegradable and convenient procedure using Aspergillus flavus as a reducing and capping agent. Research on new, simple, rapid, eco-friendly and cheaper methods has been initiated. TiO(2) NPs were characterized by FTIR, XRD, AFM, SEM and TEM studies. The X-ray diffraction showed the presence of increased amount of TiO(2) NPs which can state by the presence of peaks at rutile peaks at 100, 002, 100 and anatase forms at 101 respectively. SEM observations revealed that synthesized TiO(2) NPs were spherical, oval in shape; individual nanoparticles as well as a few aggregate having the size of 62-74 nm. AFM shows crystallization temperature was seen on the roughness of the surface of TiO(2). The Minimum inhibitory concentration value for the synthesized TiO(2) NPs was found to be 40 μg ml(-1) for Escherichia coli, which was corresponding to the value of well diffusion test. This is the first report on antimicrobial activity of fungus-mediated synthesized TiO(2) NPs, which was proved to be a good novel antibacterial material.
authors.affiliation: Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C.Abdul Hakeem College, Melvisharam 632 509, Vellore District, Tamil Nadu, India.
authors.forename: G
authors.initials: G
authors.lastname: Rajakumar
authors.forename: A Abdul
authors.initials: AA
authors.lastname: Rahuman
authors.forename: S Mohana
authors.initials: SM
authors.lastname: Roopan
authors.forename: V Gopiesh
authors.initials: VG
authors.lastname: Khanna
authors.forename: G
authors.initials: G
authors.lastname: Elango
authors.forename: C
authors.initials: C
authors.lastname: Kamaraj
authors.forename: A Abduz
authors.initials: AA
authors.lastname: Zahir
authors.forename: K
authors.initials: K
authors.lastname: Velayutham
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D001231:Aspergillus flavus / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D004926:Escherichia coli / Q000187:drug effects; D004927:Escherichia coli Infections / Q000188:drug therapy; D053758:Nanoparticles / Q000737:chemistry / Q000382:microbiology* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods*; D010084:Oxidation-Reduction; D017550:Spectroscopy, Fourier Transform Infrared; D014025:Titanium / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D014961:X-Ray Diffraction
chemical_list: D000900:Anti-Bacterial Agents; C009495:titanium dioxide; D014025:Titanium