title: Osteoadherin accumulates in the predentin towards the mineralization front in the developing tooth.
abstract: BACKGROUND
  Proteoglycans (PG) are known to be involved in the organization and assembly of the extracellular matrix (ECM) prior to mineral deposition. Osteoadherin (OSAD), a keratan sulphate PG is a member of the small leucine-rich (SLRP) family of PGs and unlike other SLRPs, OSAD expression is restricted to mineralized tissues. It is proposed to have a high affinity for hydroxyapatite and has been shown to be expressed by mature osteoblasts but its exact role remains to be elucidated.
  
  
  RESULTS
  We investigated the protein distribution of OSAD in the developing mouse tooth using immunohistochemistry and compared its expression with other SLRPs, biglycan (BGN), decorin (DCN) and fibromodulin (FMD). OSAD was found to be specifically localized in the predentin layer of the tooth and focused at the mineralization front. These studies were confirmed at the ultrastructural level using electron microscopy (iEM), where the distribution of immunogold labeled OSAD particles were quantified and significant amounts were found in the predentin, forming a gradient towards the mineralization front. In addition, iEM results revealed OSAD to lie in close association with collagen fibers, further suggesting an important role for OSAD in the organization of the ECM. The expression profile of mineralization-related SLRP genes by rat dental pulp cells exposed to mineralization inducing factors, showed an increase in all SLRP genes. Indeed, OSAD expression was significantly increased during the mineralization process, specifically following, matrix maturation, and finally mineral deposition. Alizarin Red S staining for calcium deposition showed clear bone-like nodules, which support matrix maturation and mineralization.
  
  
  CONCLUSIONS
  These studies provide new evidence for the role of OSAD in the mineralization process and its specific localization in the predentin layer accumulating at the mineralization front highlighting its role in tooth development.
authors.affiliation: Oral Biology, Department of Dental Medicine, Karolinska Institutet, Huddinge, Sweden.
authors.forename: Hero
authors.initials: H
authors.lastname: Nikdin
authors.forename: Marie-Louise
authors.initials: ML
authors.lastname: Olsson
authors.forename: Kjell
authors.initials: K
authors.lastname: Hultenby
authors.forename: Rachael V
authors.initials: RV
authors.lastname: Sugars
mesh_terms: D000818:Animals; D058578:Biglycan / Q000378:metabolism; D015153:Blotting, Western; D049109:Cell Proliferation; D002478:Cells, Cultured; D058575:Decorin / Q000378:metabolism; D003804:Dentin / Q000378:metabolism* / Q000648:ultrastructure; D003810:Dentinogenesis / Q000502:physiology*; D005109:Extracellular Matrix / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D000071235:Fibromodulin; D007124:Immunoenzyme Techniques; D051379:Mice; D011509:Proteoglycans / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014070:Tooth / Q000196:embryology* / Q000378:metabolism*; D014074:Tooth Calcification / Q000502:physiology*
chemical_list: D058578:Biglycan; D058575:Decorin; D016326:Extracellular Matrix Proteins; C000604332:Fmod protein, mouse; C000604334:Fmod protein, rat; D011509:Proteoglycans; D012333:RNA, Messenger; C113360:osteoadherin; D000071235:Fibromodulin