title: Automation of cell-based drug absorption assays in 96-well format using permeable support systems.
abstract: Cell-based drug absorption assays, such as Caco-2 and MDCK-MDR1, are an essential component of lead compound ADME/Tox testing. The permeability and transport data they provide can determine whether a compound continues in the drug discovery process. Current methods typically incorporate 24-well microplates and are performed manually. Yet the need to generate absorption data earlier in the drug discovery process, on an increasing number of compounds, is driving the use of higher density plates. A simple, more efficient process that incorporates 96-well permeable supports and proper instrumentation in an automated process provides more reproducible data compared to manual methods. Here we demonstrate the ability to perform drug permeability and transport assays using Caco-2 or MDCKII-MDR1 cells. The assay procedure was automated in a 96-well format, including cell seeding, media and buffer exchanges, compound dispense, and sample removal using simple robotic instrumentation. Cell monolayer integrity was confirmed via transepithelial electrical resistance and Lucifer yellow measurements. Proper cell function was validated by analyzing apical-to-basolateral and basolateral-to-apical movement of rhodamine 123, a known P-glycoprotein substrate. Apparent permeability and efflux data demonstrate how the automated procedure provides a less variable method than manual processing, and delivers a more accurate assessment of a compound's absorption characteristics.
authors.affiliation: BioTek Instruments, Inc., Winooski, VT 05404, USA. larsonb@biotek.com
authors.forename: Brad
authors.initials: B
authors.lastname: Larson
authors.forename: Peter
authors.initials: P
authors.lastname: Banks
authors.forename: Hilary
authors.initials: H
authors.lastname: Sherman
authors.forename: Mark
authors.initials: M
authors.lastname: Rothenberg
mesh_terms: D000818:Animals; D057205:Automation, Laboratory; D018938:Caco-2 Cells; D018929:Cell Culture Techniques; D002463:Cell Membrane Permeability; D002465:Cell Movement; D004285:Dogs; D004353:Drug Evaluation, Preclinical / Q000295:instrumentation* / Q000379:methods*; D017097:Electric Impedance; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D057166:High-Throughput Screening Assays; D006801:Humans; D008904:Miniaturization; D010599:Pharmacokinetics