title: Comparison of the (CCG)4-based PCR and MIRU-VNTR for molecular typing of Mycobacterium avium strains.
abstract: Mycobacterium avium, a member of the group of non-tuberculous mycobacteria, is most often responsible for the serious diseases in humans and is frequently isolated from NTM-caused pulmonary events. In this connection the epidemiological aspect is also of great importance. Here we present a useful genetic assay that uses (CCG)(4)-based PCR for genotyping M. avium. After applying this test to 33 strains of M. avium, we found a discriminatory index of 0.979. The accuracy of this analysis was supported by a reasonable reproducibility of 95.1%. These results were compared with the Mycobacterial Intergenic Repeat Unit-Variable Number Tandem Repeats (MIRU-VNTR) typing scheme which had slightly lower discriminatory index of 0.945 however, the method was able to cluster different strains compared to CCG-PCR. Taking into account high discriminatory index and reproducibility, this test scheme has the potential as a screening tool in the investigation of M. avium infections, especially if combined with MIRU-VNTR.
authors.affiliation: Institute of Medical Biology PAS, 106 Lodowa Street, 93-232 Lodz, Poland.
authors.forename: Arkadiusz
authors.initials: A
authors.lastname: Wojtasik
authors.forename: Anna B
authors.initials: AB
authors.lastname: Kubiak
authors.forename: Anna
authors.initials: A
authors.lastname: Krzyżanowska
authors.forename: Marta
authors.initials: M
authors.lastname: Majchrzak
authors.forename: Ewa
authors.initials: E
authors.lastname: Augustynowicz-Kopeć
authors.forename: Paweł
authors.initials: P
authors.lastname: Parniewski
mesh_terms: D021901:DNA, Intergenic / Q000235:genetics*; D005838:Genotype; D006801:Humans; D018598:Minisatellite Repeats; D058889:Molecular Typing; D015269:Mycobacterium avium Complex / Q000145:classification* / Q000235:genetics*; D015270:Mycobacterium avium-intracellulare Infection / Q000175:diagnosis*; D016133:Polymerase Chain Reaction / Q000379:methods
chemical_list: D021901:DNA, Intergenic