title: Using a single blood sample and inulin to estimate glomerular filtration rate in rabbits.
abstract: To establish a simple procedure for estimating the glomerular filtration rate (GFR) in conscious rabbits, we used the conventional multisample approach to develop a single-blood-sample method. A bolus injection of inulin was administered intravenously at a dose of 40 mg/kg to male New  Zealand White rabbits, and blood was collected 30, 60, 90, and 120 min later. Serum inulin, urea nitrogen, and creatinine concentrations were determined. Using this multi-sample method, the reference GFR in clinically healthy rabbits was 4.01 ± 0.17 mL/min/kg (n = 17). In rabbits  given an intravenous injection of the antitumor agent cisplatin, GFR fell before serum urea nitrogen and creatinine concentrations increased. Based on cumulative GFR data from healthy and nephropathy rabbits, the GFR obtained from the 3-sample method (30-, 60-, and 90-min samples) was closely  correlated (r = 0.99) with that calculated from the estimated distribution volume and serum inulin concentration at 90 min after inulin injection in the single-blood-sample method. These results demonstrate that the single-blood-sample method supports sequential GFR measurements in  rabbits and is a versatile procedure not only for research purposes but also in clinical settings.
authors.affiliation: Department of Veterinary Basic Medicine, Iwate University, Morioka, Japan.
authors.forename: Yuuki
authors.initials: Y
authors.lastname: Michigoshi
authors.forename: Norio
authors.initials: N
authors.lastname: Yamagishi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Satoh
authors.forename: Masaki
authors.initials: M
authors.lastname: Kato
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Furuhama
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000830:Animals, Laboratory; D001800:Blood Specimen Collection; D001806:Blood Urea Nitrogen; D002945:Cisplatin; D003404:Creatinine / Q000097:blood; D005919:Glomerular Filtration Rate / Q000662:veterinary*; D007444:Inulin / Q000008:administration & dosage / Q000097:blood; D008297:Male; D011817:Rabbits; D013997:Time Factors
chemical_list: D007444:Inulin; D003404:Creatinine; D002945:Cisplatin