title: [A novel severe aplastic anemia mouse model induced by IFN-γ plus busulphan].
abstract: OBJECTIVE
  To establish a novel severe aplastic anemia (SAA) mouse model by interferon-γ (IFN-γ) plus busulphan.
  
  
  METHODS
  Thirty clean-class BALB/c female mice were intraperitoneally injected with IFN-γ and intragastrically administrated with busulphan (group I), meanwhile busulphan alone group (n = 30, group II) and normal control group (n = 30, group III). Multi-parameters were compared among the three groups.
  
  
  RESULTS
  In group I at day 10 after treatment, the incidence of SAA was 100% and mortality 20% respectively; the WBC, HGB, PLT, absolute reticulocyte count (Ret) and tibial nucleated cell count (TNCC) were (0.8 ± 0.3) × 10(9)/L, (45 ± 20) g/L, (10 ± 8) × 10(9)/L, (15.2 ± 10.2) × 10(9)/L, (12 ± 7) × 10(6)/tibia, respectively, which were significantly different from the other two groups (all P < 0.05). The bone marrow smears and patho-histological examinations showed marked reductions of marrow cell proliferation, and increases of the percentages of non-hematopoietic cells and cellular adipose. The depression was severe and irreversible. In group II, the blood cells count, TNCC and marrow proliferation recovered gradually with erythroid hyperplasia and hematopoietic dysplasia.
  
  
  CONCLUSIONS
  IFN-γ plus busulphan can establish a SAA mouse model in a relatively short period, which is more resemble with human SAA.
authors.affiliation: Department of Hematology, Nantong University, Nantong, China.
authors.forename: Fang-ying
authors.initials: FY
authors.lastname: Ning
authors.forename: Zeng-hua
authors.initials: ZH
authors.lastname: Lin
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Xiu-fang
authors.initials: XF
authors.lastname: Chen
authors.forename: Feng
authors.initials: F
authors.lastname: Sun
authors.forename: Han
authors.initials: H
authors.lastname: Wang
authors.forename: Hai-yan
authors.initials: HY
authors.lastname: Liu
mesh_terms: D000741:Anemia, Aplastic / Q000139:chemically induced*; D000818:Animals; D002066:Busulfan / Q000009:adverse effects*; D004195:Disease Models, Animal; D005260:Female; D007371:Interferon-gamma / Q000009:adverse effects*; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D007371:Interferon-gamma; D002066:Busulfan