title: A one-time inducible transposon to create knockout mutants in rice.
abstract: Use of a transposon is an efficient tagging tool for exploring the function of the gene it inserts into or is adjacent to. A few modifications have been applied to the native Ac transposon to allow it to transpose efficiently or spontaneously and stop quickly thereafter. Furthermore, locating the transposon between a constitutive plant promoter and a reporter gene, such as the firefly luciferase gene, allows for nondestructively detecting excision events in vivo. This chapter describes a detailed protocol for one-time inducible transposon tagging of rice cells and their subsequent screening and regeneration into mutant lines.
authors.affiliation: Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China. bocharng@ntu.edu.tw
authors.forename: Yuh-Chyang
authors.initials: YC
authors.lastname: Charng
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics*; D055786:Gene Knockout Techniques; D017930:Genes, Reporter; D049409:Luciferases, Firefly / Q000235:genetics; D016254:Mutagenesis, Insertional; D009154:Mutation; D012275:Oryza / Q000235:genetics*; D011401:Promoter Regions, Genetic; D020156:Salicylic Acid
chemical_list: D004251:DNA Transposable Elements; D049409:Luciferases, Firefly; D020156:Salicylic Acid