title: Th17 responses are not induced in dextran sodium sulfate model of acute colitis.
abstract: Dextran sodium sulfate (DSS) is a widely used chemical model for inflammatory bowel disease (IBD). It is thought that imbalances in the T helper (Th) cell subsets contribute to IBD. Recent studies suggest that the acute DSS-colitis model is polarized toward a Th1/Th17 profile based on RT-PCR analysis of colonic tissues. In the current study we determined whether colonic Th cells from DSS-colitis mice were skewed toward the Th17 profile. Mice were treated with 5% DSS for 7 days and colonic T cells isolated and examined for production of IFN-γ (Th1 cell), IL-4 (Th2 cell) and IL-17 (Th17 cell) by intracellular flow cytometry. We found that the percentage of colonic Th17 cells were similar to non-treated controls but the percentage of Th1 cells were elevated in DSS-colitis mice. These results suggest that in the acute DSS-colitis model the colonic Th cells exhibit a Th1 profile and not a Th17 profile.
authors.affiliation: Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University at Wonju, Wonju 220-710, Korea.
authors.forename: Yoon Suk
authors.initials: YS
authors.lastname: Kim
authors.forename: Min Ho
authors.initials: MH
authors.lastname: Lee
authors.forename: Ahn Seung
authors.initials: AS
authors.lastname: Ju
authors.forename: Ki-Jong
authors.initials: KJ
authors.lastname: Rhee