title: Targeting CreER(T2) expression to keratin 8-expressing murine simple epithelia using bacterial artificial chromosome transgenesis.
abstract: Keratin 8 (K8) is a type II keratin that is associated with the type I keratins K18 or K19 in single layered epithelia. We generated a bacterial artificial chromosome (BAC) transgenic mouse line that expresses the tamoxifen inducible CreER(T2) inserted into the endogenous murine K8 gene. The transgenic mouse line contains two copies of the BAC transgene. To determine the expression specificity and inducibility of CreER(T2), the K8-CreER(T2) mice were bred with a Gt(ROSA                         26)(                            ACTB-tdTomato-EGFP                         ) fluorescent protein-based reporter transgenic mouse line. We demonstrated that CreER(T2) and the endogenous K8 gene share the same patterns of expression and that the enzymatic activity of CreER(T2) can be efficiently induced by tamoxifen in all K8-expressing tissues. This mouse line will be useful for studying gene function in development and homeostasis of simple epithelia, and investigating both tissue lineage hierarchy and the identity of the cells of origin for epithelial cancers.
authors.affiliation: Department of Molecular and Cellular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Boyu
authors.initials: B
authors.lastname: Zhang
authors.forename: Sang Jun
authors.initials: SJ
authors.lastname: Han
authors.forename: Amy N
authors.initials: AN
authors.lastname: Shore
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Rosen
authors.forename: Francesco J
authors.initials: FJ
authors.lastname: Demayo
authors.forename: Li
authors.initials: L
authors.lastname: Xin
mesh_terms: D000818:Animals; D022202:Chromosomes, Artificial, Bacterial / Q000378:metabolism*; D004789:Enzyme Activation; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D018390:Gene Targeting / Q000379:methods; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D060005:Genotyping Techniques; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007150:Immunohistochemistry; D019426:Integrases / Q000235:genetics / Q000378:metabolism*; D053533:Keratin-8 / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008856:Microscopy, Fluorescence; D013629:Tamoxifen / Q000378:metabolism / Q000494:pharmacology; D019076:Transgenes
chemical_list: D053533:Keratin-8; C486595:Krt8 protein, mouse; D013629:Tamoxifen; D049452:Green Fluorescent Proteins; C045073:Cre recombinase; D019426:Integrases