title: Coupling in vitro gastrointestinal lipolysis and Caco-2 cell cultures for testing the absorption of different food emulsions.
abstract: There is a growing interest in the optimization of dietary emulsions for monitoring postprandial lipid metabolism in the frame of preventing metabolic diseases. Using various emulsions, we investigated in a systematic scheme the combination of (i) in vitro gastrointestinal lipolysis and (ii) absorption and metabolism of lipolysis media in Caco-2 cells. Four emulsions based on either milk fat olein (OL) or rapeseed oil (RA) as the dispersed phase and either lecithin (LE) or sodium caseinate (CA) as the emulsifier were tested. After a sequential incubation of these emulsions with gastric and pancreatic enzymes, lipolysis media were incubated with Caco-2 cells, after dilution (1 : 20) to maintain the barrier integrity. Both gastric and duodenal lipolysis levels were similar to values reported in vivo and the rates of lipolysis were higher with LE-stabilized emulsions than with CA-stabilized emulsions (P < 0.05). TAG secretion by Caco-2 cells was found to be higher using (i) duodenal vs. gastric media (P < 0.001) and (ii) emulsions stabilized with CA vs. LE (P < 0.01). Consistently, gene expression of both FABP2 and FATP4 induced by the duodenal media was (i) higher than that with gastric media (P < 0.001) and (ii) faster than that with model mixed micelles. Using gastric media, TAG secretion of Caco-2 cells after 12 h was higher with RA than with OL (P < 0.001). Moreover, the RA-CA emulsion increased the size of secreted lipoprotein particles (514 nm vs. 61 to 130 nm; P < 0.01). In conclusion, it was possible to observe distinct responses in the lipid metabolism of Caco-2 cells incubated with lipolysis media obtained from different dietary emulsions digested by gastrointestinal lipases in vitro.
authors.affiliation: INRA USC1235, CarMeN laboratory, INSA-Lyon, IMBL, Villeurbanne, F-69621, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Vors
authors.forename: Perrine
authors.initials: P
authors.lastname: Capolino
authors.forename: Clémence
authors.initials: C
authors.lastname: Guérin
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Meugnier
authors.forename: Sandra
authors.initials: S
authors.lastname: Pesenti
authors.forename: Marie-Agnès
authors.initials: MA
authors.lastname: Chauvin
authors.forename: Julien
authors.initials: J
authors.lastname: Monteil
authors.forename: Noël
authors.initials: N
authors.lastname: Peretti
authors.forename: Maud
authors.initials: M
authors.lastname: Cansell
authors.forename: Frédéric
authors.initials: F
authors.lastname: Carrière
authors.forename: Marie-Caroline
authors.initials: MC
authors.lastname: Michalski
mesh_terms: D018938:Caco-2 Cells; D002914:Chylomicrons / Q000378:metabolism; D004041:Dietary Fats / Q000032:analysis / Q000378:metabolism*; D045703:Emulsifying Agents / Q000737:chemistry / Q000378:metabolism; D004655:Emulsions; D020895:Enterocytes / Q000378:metabolism*; D050612:Fatty Acid Transport Proteins / Q000235:genetics / Q000378:metabolism; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005240:Feasibility Studies; D005524:Food Technology; D005750:Gastric Juice / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D007408:Intestinal Absorption; D007700:Kinetics; D008066:Lipolysis; D008954:Models, Biological; D010189:Pancreatic Juice / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism*; D010316:Particle Size; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D002914:Chylomicrons; D004041:Dietary Fats; D045703:Emulsifying Agents; D004655:Emulsions; C493404:FABP2 protein, human; D050612:Fatty Acid Transport Proteins; D050556:Fatty Acid-Binding Proteins; D012333:RNA, Messenger; C493571:SLC27A4 protein, human