title: Gas chromatography-mass spectrometry method for the determination of free amino acids as their dimethyl-tert-butylsilyl (TBDMS) derivatives in animal source food.
abstract: The suitability of a one-step derivatization procedure using N-methyl-N-(tert-butyldimethylsilyl)trifluoroacetamide for the simultaneous assay of 22 free amino acids and its application for their analysis in six animal source foods (pork, dry cured ham, chicken stock, fresh cheese, ripened cheese, and dry salted sardine) by GC-MS were studied. All 22 free amino acid derivatives were correctly detected and resolved. Reproducibility (%RSD) of the method was in the range of 1.9-12.2%. Detection and quantitation limits of the analytical procedure ranged from 0.01 to 0.46 mg/100 g dry weight and from 0.02 to 1.55 mg/100 g dry weight, respectively. The calibration curves were linear within the range 0.1-15.0 mg/100 g with correlation coefficient values (R(2)) from 0.9891 to 0.9983. All analyzed food products showed free amino acid contents similar to those found in the scientific literature. The proposed GC-MS method for the determination of free amino acids in animal source food can be used in routine for both analytical and research purposes.
authors.affiliation: Department of Animal Prodution and Food Science (Food Technology), Faculty of Veterinary Sciences, University of Extremadura, Cáceres, Spain.
authors.forename: Estefanía
authors.initials: E
authors.lastname: Jiménez-Martín
authors.forename: Jorge
authors.initials: J
authors.lastname: Ruiz
authors.forename: Trinidad
authors.initials: T
authors.lastname: Pérez-Palacios
authors.forename: Antonio
authors.initials: A
authors.lastname: Silva
authors.forename: Teresa
authors.initials: T
authors.lastname: Antequera
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D002611:Cheese / Q000032:analysis*; D002645:Chickens; D005504:Food Analysis / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D008461:Meat Products / Q000032:analysis*; D015394:Molecular Structure; D013552:Swine
chemical_list: D000596:Amino Acids