title: Rapid and simultaneous detection of linear chromosome and large plasmids in Proteobacteria.
abstract: Bacterial genomic architectures are complex and play important roles in genome evolution. While most bacterial lineage genomes contain a single chromosome often accompanied by plasmids, more and more genomes are described as harboring a linear or a second chromosome. Thus, the development of bacterial genomics leads to the study of bacterial genome architectures. In order to avoid laborious techniques combining several methods, we developed an original plug-free pulsed field gel electrophoresis procedure, that enabled us to co-characterize reproducibly the presence of linear chromosomes and plasmids ranging between 30 kb and 2000 kb in various proteobacterial lineages.
authors.affiliation: Université de Lyon; Université Lyon, Lyon, France.
authors.forename: Martha Helena
authors.initials: MH
authors.lastname: Ramírez-Bahena
authors.forename: Xavier
authors.initials: X
authors.lastname: Nesme
authors.forename: Daniel
authors.initials: D
authors.lastname: Muller
mesh_terms: D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D016680:Genome, Bacterial; D010957:Plasmids / Q000235:genetics*; D020560:Proteobacteria / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial