title: Microarray analysis of differentially expressed genes between cysts and trophozoites of Acanthamoeba castellanii.
abstract: Acanthamoeba infection is difficult to treat because of the resistance property of Acanthamoeba cyst against the host immune system, diverse antibiotics, and therapeutic agents. To identify encystation mediating factors of Acanthamoeba, we compared the transcription profile between cysts and trophozoites using microarray analysis. The DNA chip was composed of 12,544 genes based on expressed sequence tag (EST) from an Acanthamoeba ESTs database (DB) constructed in our laboratory, genetic information of Acanthamoeba from TBest DB, and all of Acanthamoeba related genes registered in the NCBI. Microarray analysis indicated that 701 genes showed higher expression than 2 folds in cysts than in trophozoites, and 859 genes were less expressed in cysts than in trophozoites. The results of real-time PCR analysis of randomly selected 9 genes of which expression was increased during cyst formation were coincided well with the microarray results. Eukaryotic orthologous groups (KOG) analysis showed an increment in T article (signal transduction mechanisms) and O article (posttranslational modification, protein turnover, and chaperones) whereas significant decrement of C article (energy production and conversion) during cyst formation. Especially, cystein proteinases showed high expression changes (282 folds) with significant increases in real-time PCR, suggesting a pivotal role of this proteinase in the cyst formation of Acanthamoeba. The present study provides important clues for the identification and characterization of encystation mediating factors of Acanthamoeba.
authors.affiliation: Department of Parasitology, Kyungpook National University School of Medicine, Daegu 700-422, Korea.
authors.forename: Eun-Kyung
authors.initials: EK
authors.lastname: Moon
authors.forename: Ying-Hua
authors.initials: YH
authors.lastname: Xuan
authors.forename: Dong-Il
authors.initials: DI
authors.lastname: Chung
authors.forename: Yeonchul
authors.initials: Y
authors.lastname: Hong
authors.forename: Hyun-Hee
authors.initials: HH
authors.lastname: Kong
mesh_terms: D048248:Acanthamoeba castellanii / Q000235:genetics* / Q000502:physiology; D000818:Animals; D016000:Cluster Analysis; D030541:Databases, Genetic; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D033661:Oocysts / Q000502:physiology*; D015800:Protozoan Proteins / Q000235:genetics*; D016053:RNA, Protozoan / Q000235:genetics; D053058:Trophozoites / Q000502:physiology*
chemical_list: D015800:Protozoan Proteins; D016053:RNA, Protozoan