title: Cdc14b regulates mammalian RNA polymerase II and represses cell cycle transcription.
abstract: Cdc14 is an essential phosphatase in yeast but its role in the mammalian cell cycle remains obscure. We report here that Cdc14b-knockout cells display unscheduled induction of multiple cell cycle regulators resulting in early entry into DNA replication and mitosis from quiescence. Cdc14b dephosphorylates Ser5 at the C-terminal domain (CTD) of RNA polymerase II, a major substrate of cyclin-dependent kinases. Lack of Cdc14b results in increased CTD-Ser5 phosphorylation, epigenetic modifications that mark active chromatin, and transcriptional induction of cell cycle regulators. These data suggest a function for mammalian Cdc14 phosphatases in the control of transcription during the cell cycle.
authors.affiliation: Cell Division and Cancer Group, Spanish National Cancer Research Center (CNIO), E-28029 Madrid, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Guillamot
authors.forename: Eusebio
authors.initials: E
authors.lastname: Manchado
authors.forename: Massimo
authors.initials: M
authors.lastname: Chiesa
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Gómez-López
authors.forename: David G
authors.initials: DG
authors.lastname: Pisano
authors.forename: María P
authors.initials: MP
authors.lastname: Sacristán
authors.forename: Marcos
authors.initials: M
authors.lastname: Malumbres
mesh_terms: D000483:Alleles; D000818:Animals; D001665:Binding Sites; D002453:Cell Cycle; D002478:Cells, Cultured; D054637:Dual-Specificity Phosphatases / Q000378:metabolism*; D044127:Epigenesis, Genetic; D005091:Exons; D005347:Fibroblasts / Q000166:cytology; D015971:Gene Expression Regulation, Enzymologic; D051379:Mice; D008938:Mitosis; D010710:Phosphates / Q000737:chemistry; D010766:Phosphorylation; D012319:RNA Polymerase II / Q000378:metabolism*; D014158:Transcription, Genetic; D015533:Transcriptional Activation
chemical_list: D010710:Phosphates; D012319:RNA Polymerase II; C541950:CDC14B protein, mouse; D054637:Dual-Specificity Phosphatases