title: [The influence of inhibiting notch signal on pulmonary vascular remodeling induced by PDGF].
abstract: OBJECTIVE
  To explore the influence of inhibiting Notch signal on pulmonary vascular remodeling induced by PDGF.
  
  
  METHODS
  Vessel strips taken from healthy Wistar rats were cultured together with extrogenous PDGF, the potent smooth muscle cell proliferation stimulators, for 7 days. Some vessel strips were cultured with PDGF and gamma-secretase inhibitor DAPT, a Notch signaling inhibitor for 7 days. Vascular wall thickness, PCNA and caspase-3 positive cell rate were examined in vessel strips. The alterations of Notch 1 to 4 receptor and HERP1, 2 mRNA were discerned by FQ-PCR to observe the influence of DAPT on Notch signal. At the same time, above indexes, which were related with pulmonary vascular remodeling, were measured too.
  
  
  RESULTS
  PDGF stimulation in the cultured normal pulmonary arteries resulted in vascular medial thickness increase for about 50%, accompanied by significant increase in PCNA positive cell rate and decrease of caspase-3 positive cell rate. When DAPT were added to inhibit Notch signaling, the expression of HERP1, 2 mRNA decreased, the degrees of PDGF induced vascular medial thickness and PCNA positive cell rate increase as well as caspase-3 positive cell rate decrease were all attenuated notably.
  
  
  CONCLUSIONS
  Inhibiting Notch signal induced by gamma-secretase inhibitor lead to the suppression of pulmonary vascular remodeling induced by PDGF, suggesting inhibition of Notch signal pathway might be a novel strategy in the intervention of pulmonary hypertension.
authors.affiliation: Department of Pediatrics, West China Second Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Li-na
authors.initials: LN
authors.lastname: Qiao
authors.forename: Xian-min
authors.initials: XM
authors.lastname: Wang
authors.forename: Zhong-qiang
authors.initials: ZQ
authors.lastname: Liu
authors.forename: Xiao-qin
authors.initials: XQ
authors.lastname: Wang
authors.forename: Tong-fu
authors.initials: TF
authors.lastname: Zhou
authors.forename: Yi-min
authors.initials: YM
authors.lastname: Hua
authors.forename: Han-min
authors.initials: HM
authors.lastname: Liu
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005260:Female; D006976:Hypertension, Pulmonary / Q000473:pathology / Q000503:physiopathology; D066298:In Vitro Techniques; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000378:metabolism / Q000473:pathology*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D011651:Pulmonary Artery / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D051880:Receptors, Notch / Q000037:antagonists & inhibitors / Q000502:physiology*; D015398:Signal Transduction / Q000187:drug effects* / Q000502:physiology
chemical_list: D010982:Platelet-Derived Growth Factor; D051880:Receptors, Notch