title: Microflow-injection chemiluminescence of luminol and hypochlorite enhanced by phloxine B.
abstract: We report for the first time that the sensitivity of the luminol-hypochlorite chemiluminescence (CL) reaction was enhanced approximately 10 times by the addition of phloxine B. The maximum wavelength of CL emission shifted from 431 to 595 nm in the absence and presence, respectively, of phloxine B, suggesting that an efficient chemiluminescence resonance energy transfer occurred between a luminol donor and a phloxine B acceptor in the luminol-hypochlorite-phloxine B system. Based on this observation, a simple, rapid and sensitive microflow injection CL method, using a microchip with spiral channel configurations, was developed for the determination of hypochlorite. Under optimized conditions, a linear calibration curve (R(2) = 0.9944) over the range 0.1-10.0 µmol/L was obtained, with a detection limit of 0.025 µmol/L (S:N = 3). The relative standard deviation (RSD) was found to be 4.2% (n = 10) for 2.5 µmol/L hypochlorite. The sample consumption was only 2 μL, with a sample throughput of 90/h. The method has been used for determining trace amounts of hypochlorite in water samples with satisfactory results.
authors.affiliation: Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou, People's Republic of China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Song
authors.forename: Hong
authors.initials: H
authors.lastname: Shen
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Yin
authors.forename: Xiuzhong
authors.initials: X
authors.lastname: Wang
authors.forename: Jinhua
authors.initials: J
authors.lastname: Liu
mesh_terms: D010697:Eosine I Bluish / Q000737:chemistry*; D017022:Flow Injection Analysis; D006997:Hypochlorous Acid / Q000737:chemistry*; D049408:Luminescent Agents / Q000737:chemistry*; D008163:Luminescent Measurements / Q000295:instrumentation*; D008165:Luminol / Q000737:chemistry*
chemical_list: D049408:Luminescent Agents; D008165:Luminol; D006997:Hypochlorous Acid; D010697:Eosine I Bluish