title: Quantitation of malondialdehyde in gingival crevicular fluid by a high-performance liquid chromatography-based method.
abstract: Lipid peroxidation (LPO) has been associated with periodontal disease, and the evaluation of malondialdehyde (MDA) in the gingival crevicular fluid (GCF), an inflammatory exudate from the surrounding tissue of the periodontium, may be useful to clarify the role of LPO in the pathogenesis of periodontal disease. We describe the validation of a method to measure MDA in the GCF using high-performance liquid chromatography. MDA calibration curves were prepared with phosphate-buffered solution spiked with increasing known concentrations of MDA. Healthy and diseased GCF samples were collected from the same patient to avoid interindividual variability. MDA response was linear in the range measured, and excellent agreement was observed between added and detected concentrations of MDA. Samples' intra- and interday coefficients of variation were below 6.3% and 12.4%, respectively. The limit of quantitation (signal/noise=5) was 0.03 μM. When the validated method was applied to the GCF, excellent agreement was observed in the MDA quantitation from healthy and diseased sites, and diseased sites presented more MDA than healthy sites (P<0.05). In this study, a validated method for MDA quantitation in GCF was established with satisfactory sensitivity, precision, and accuracy.
authors.affiliation: Department of Diagnosis and Surgery, Araraquara School of Dentistry, Universidade Estadual Paulista, Araraquara, São Paulo 14801-903, Brazil.
authors.forename: Alliny Souza
authors.initials: AS
authors.lastname: Bastos
authors.forename: Ana Paula de Melo
authors.initials: AP
authors.lastname: Loureiro
authors.forename: Tiago Franco
authors.initials: TF
authors.lastname: de Oliveira
authors.forename: Sâmia Cruz Tfaile
authors.initials: SC
authors.lastname: Corbi
authors.forename: Raquel Mantuaneli Scarel
authors.initials: RM
authors.lastname: Caminaga
authors.forename: Carlos Rossa
authors.initials: CR
authors.lastname: Júnior
authors.forename: Silvana R P
authors.initials: SR
authors.lastname: Orrico
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D006801:Humans; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000032:analysis*; D010510:Periodontal Diseases / Q000378:metabolism / Q000473:pathology
chemical_list: D008315:Malondialdehyde