title: Characterization of a new insect cell line that is derived from the neonate larvae of Papilio xuthus (Lepidoptera: Papilionidae) and its susceptibility to AcNPV.
abstract: The cell line RIRI-PX1 was established from neonate larval tissues of Papilio xuthus by performing primary cultures in the modified Grace medium that was supplemented with 20% fetal bovine serum (FBS). The cell line primarily consisted of spindle-shaped and spherical cells which attached themselves to the flask. The population-doubling times (PDTs) at the 50th and 60th passage were 42.5 h and 42.1 h respectively. The average chromosome numbers of RIRI-PX1 cell line from passage 5 to passage 50 ranged from 103 to 199. It was confirmed that RIRI-PX1 cell line was derived from P. xuthus by comparing the mitochondrial cytochrome c oxidase subunit I gene (COI) of RIRI-PX1 cells and P. xuthus eggs. This cell line was susceptible to the Autographa californica nucleopolyhedrovirus (AcNPV) and produced high yield of polyhedral occlusion bodies (43.9OBs/cell) after 10 days of infection by AcNPV. The virus titer of AcNPV infected RIRI-PX1 cells was 3.25×10⁷ TCID₅₀/ml. We concluded that the RIRI-PX1 cell line is established from the neonate larvae tissues successfully and the cells of the cell line are sensitive to AcNPV.
authors.affiliation: Research Institute of Resource Insects, Chinese Academy of Forestry, The Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration, Kunming 650224, Yunnan, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Feng
authors.forename: Wei-Feng
authors.initials: WF
authors.lastname: Ding
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Chen
authors.forename: Cheng-Ye
authors.initials: CY
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Ma
mesh_terms: D000818:Animals; D001483:Base Sequence; D002080:Butterflies / Q000166:cytology* / Q000235:genetics / Q000254:growth & development / Q000821:virology; D002460:Cell Line; D059006:Chromosomes, Insect / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics; D017344:Genes, Insect; D007814:Larva / Q000166:cytology / Q000235:genetics / Q000254:growth & development; D008928:Mitochondria / Q000235:genetics; D008969:Molecular Sequence Data; D017924:Nucleopolyhedroviruses / Q000254:growth & development / Q000472:pathogenicity*; D061251:Primary Cell Culture / Q000379:methods*; D013997:Time Factors; D019562:Viral Load
chemical_list: D003576:Electron Transport Complex IV