title: Combined chromatin and expression analysis reveals specific regulatory mechanisms within cytokine genes in the macrophage early immune response.
abstract: Macrophages play a critical role in innate immunity, and the expression of early response genes orchestrate much of the initial response of the immune system. Macrophages undergo extensive transcriptional reprogramming in response to inflammatory stimuli such as Lipopolysaccharide (LPS).To identify gene transcription regulation patterns involved in early innate immune responses, we used two genome-wide approaches--gene expression profiling and chromatin immunoprecipitation-sequencing (ChIP-seq) analysis. We examined the effect of 2 hrs LPS stimulation on early gene expression and its relation to chromatin remodeling (H3 acetylation; H3Ac) and promoter binding of Sp1 and RNA polymerase II phosphorylated at serine 5 (S5P RNAPII), which is a marker for transcriptional initiation. Our results indicate novel and alternative gene regulatory mechanisms for certain proinflammatory genes. We identified two groups of up-regulated inflammatory genes with respect to chromatin modification and promoter features. One group, including highly up-regulated genes such as tumor necrosis factor (TNF), was characterized by H3Ac, high CpG content and lack of TATA boxes. The second group, containing inflammatory mediators (interleukins and CCL chemokines), was up-regulated upon LPS stimulation despite lacking H3Ac in their annotated promoters, which were low in CpG content but did contain TATA boxes. Genome-wide analysis showed that few H3Ac peaks were unique to either +/-LPS condition. However, within these, an unpacking/expansion of already existing H3Ac peaks was observed upon LPS stimulation. In contrast, a significant proportion of S5P RNAPII peaks (approx 40%) was unique to either condition. Furthermore, data indicated a large portion of previously unannotated TSSs, particularly in LPS-stimulated macrophages, where only 28% of unique S5P RNAPII peaks overlap annotated promoters. The regulation of the inflammatory response appears to occur in a very specific manner at the chromatin level for specific genes and this study highlights the level of fine-tuning that occurs in the immune response.
authors.affiliation: Atherosclerosis Research Unit, Department of Medicine, Centre for Molecular Medicine, Karolinska Institute, Stockholm, Sweden. maria.iglesias@ki.se
authors.forename: Maria Jesus
authors.initials: MJ
authors.lastname: Iglesias
authors.forename: Maria
authors.initials: M
authors.lastname: Jesus Iglesias
authors.forename: Sarah-Jayne
authors.initials: SJ
authors.lastname: Reilly
authors.forename: Olof
authors.initials: O
authors.lastname: Emanuelsson
authors.forename: Bengt
authors.initials: B
authors.lastname: Sennblad
authors.forename: Mohammad
authors.initials: M
authors.lastname: Pirmoradian Najafabadi
authors.forename: Lasse
authors.initials: L
authors.lastname: Folkersen
authors.forename: Anders
authors.initials: A
authors.lastname: Mälarstig
authors.forename: Jens
authors.initials: J
authors.lastname: Lagergren
authors.forename: Per
authors.initials: P
authors.lastname: Eriksson
authors.forename: Anders
authors.initials: A
authors.lastname: Hamsten
authors.forename: Jacob
authors.initials: J
authors.lastname: Odeberg
mesh_terms: D002454:Cell Differentiation; D002843:Chromatin / Q000737:chemistry*; D047369:Chromatin Immunoprecipitation; D018899:CpG Islands; D016207:Cytokines / Q000378:metabolism*; D020869:Gene Expression Profiling; D055106:Genome-Wide Association Study; D006657:Histones / Q000737:chemistry; D006801:Humans; D007107:Immune System; D007113:Immunity, Innate; D007249:Inflammation / Q000235:genetics; D008264:Macrophages / Q000166:cytology / Q000378:metabolism*; D008954:Models, Biological; D009000:Monocytes / Q000166:cytology; D005810:Multigene Family; D020411:Oligonucleotide Array Sequence Analysis; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012333:RNA, Messenger / Q000378:metabolism; D012694:Serine / Q000737:chemistry
chemical_list: D002843:Chromatin; D016207:Cytokines; D006657:Histones; D012333:RNA, Messenger; D012694:Serine