title: Influence of MMP-8 promoter polymorphism in early osseointegrated implant failure.
abstract: OBJECTIVE
  Dental implants consist in the treatment of choice to replace tooth loss. The knowledge that implant loss tends to cluster in subsets of individuals may indicate that host immuneinflammatory response is influenced by genetic factors. In fact, genetic polymorphisms influence the osseointegration process. The objective of this study was investigate the possible relationship between C-799T polymorphism in matrix metalloproteinase 8 (MMP-8) gene and early implant failure in nonsmoker patients.
  
  
  METHODS
  Subjects were divided into two groups: control group (100 patients with one or more healthy implants) and test group (80 patients that had suffered one or more early implant failures). Genomic DNA from oral mucosa was amplified by PCR and analyzed by restriction endonucleases. The significance of the differences in observed frequencies of polymorphisms was assessed by Chi-square.
  
  
  RESULTS
  Statistical analysis shows that in the MMP-8 gene, the T allele in 76.25% in the test group and the T/T genotype, 63.75% in the same group, may predispose to early loss of implants osseointegrated.
  
  
  CONCLUSIONS
  These results suggest that polymorphism in the promoter region of MMP-8 gene is associated with early implant failure. This polymorphism can be a genetic marker to risk of implant loss.
  
  
  CONCLUSIONS
  The determination of this genetic pattern in osseointegration would enable the identification of individuals at higher risk to loss implant. Thus, genetic markers will be identified, contributing to an appropriate preoperative selection and preparation of strategies for prevention and therapy individualized to modulate the genetic markers and increase the success rate of treatments.
authors.affiliation: Centro Politécnico, Setor de Ciências Biológicas, Departamento de Biologia Celular, University Federal of Paraná, Jardim das Américas, Curitiba, Paraná, Brazil, 81531-990.
authors.forename: F R
authors.initials: FR
authors.lastname: Costa-Junior
authors.forename: C C
authors.initials: CC
authors.lastname: Alvim-Pereira
authors.forename: F
authors.initials: F
authors.lastname: Alvim-Pereira
authors.forename: P C
authors.initials: PC
authors.lastname: Trevilatto
authors.forename: A P
authors.initials: AP
authors.lastname: de Souza
authors.forename: Maria Cristina L G
authors.initials: MC
authors.lastname: Santos
mesh_terms: D000483:Alleles; D020029:Base Pairing / Q000235:genetics; D003596:Cytosine; D015921:Dental Implants; D019232:Dental Restoration Failure; D005787:Gene Frequency / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006801:Humans; D020784:Matrix Metalloproteinase 8 / Q000235:genetics*; D016348:Osseointegration / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012307:Risk Factors; D013941:Thymine
chemical_list: D015921:Dental Implants; D005819:Genetic Markers; D003596:Cytosine; C488762:MMP8 protein, human; D020784:Matrix Metalloproteinase 8; D013941:Thymine