title: [Molecular genetic characteristics of the Wx-B1e allele from common wheat and applicability of the DNA markers for its identification].
abstract: Molecular genetic characterization of the Wx-B1e allele identified by the authors of the study in the common wheat cultivar Korotyshka was performed. The 804-bp Wx-B1e fragment was cloned and sequenced. Comparison of the sequence obtained with that for the wild-type allele of common wheat (Wx-B1a) demonstrated that Wx-B1e carried the 34-bp insertion, 8-bp deletion, and 23 nucleotide substitutions. BLAST analysis revealed the highest homology with the nucleotide sequences of Wx genes from Triticum spelta and Triticum durum. The amplification variants of four Wx-B1 molecular markers, applied worldwide for testing the collections for different Wx allelic variants, are demonstrated.
authors.forename: M G
authors.initials: MG
authors.lastname: Divashchuk
authors.forename: M V
authors.initials: MV
authors.lastname: Klimushina
authors.forename: G I
authors.initials: GI
authors.lastname: Karlov
mesh_terms: D000483:Alleles; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000235:genetics*; D017343:Genes, Plant; D005819:Genetic Markers; D009154:Mutation; D014908:Triticum / Q000235:genetics*
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers