title: Rapid microarray processing using a disposable hybridization chamber with an integrated micropump.
abstract: We present a disposable microarray hybridization chamber with an integrated micropump to speed up diffusion based reaction kinetics by generating convective flow. The time-to-result for the hybridization reaction was reduced from 60 min (standard protocol) down to 15 min for a commercially available microarray. The integrated displacement micropump is pneumatically actuated. It includes two active microvalves and is designed for low-cost, high volume manufacturing. The setup is made out of two microstructured polymer parts realized in polycarbonate (PC) separated by a 25 μm thermoplastic elastomer (TPE) membrane. Pump rate can be controlled between 0.3 μl s(-1) and 5.7 μl s(-1) at actuation frequencies between 0.2 Hz and 8.0 Hz, respectively.
authors.affiliation: Robert Bosch GmbH, Corporate Sector Research Microsystem Technologies, Robert Bosch Platz 1, 70839 Gerlingen, Germany. Jochen.Rupp3@de.bosch.com
authors.forename: Jochen
authors.initials: J
authors.lastname: Rupp
authors.forename: Manuela
authors.initials: M
authors.lastname: Schmidt
authors.forename: Susanne
authors.initials: S
authors.lastname: Münch
authors.forename: Markus
authors.initials: M
authors.lastname: Cavalar
authors.forename: Ulf
authors.initials: U
authors.lastname: Steller
authors.forename: Jürgen
authors.initials: J
authors.lastname: Steigert
authors.forename: Michael
authors.initials: M
authors.lastname: Stumber
authors.forename: Christian
authors.initials: C
authors.lastname: Dorrer
authors.forename: Peter
authors.initials: P
authors.lastname: Rothacher
authors.forename: Roland
authors.initials: R
authors.lastname: Zengerle
authors.forename: Martina
authors.initials: M
authors.lastname: Daub
mesh_terms: D004247:DNA / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry; D007700:Kinetics; D008567:Membranes, Artificial; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D011076:Polycarboxylate Cement / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D008567:Membranes, Artificial; D011076:Polycarboxylate Cement; C005506:polycarbonate; D004247:DNA