title: The obesity and fatty liver are reduced by plant-derived Pediococcus pentosaceus LP28 in high fat diet-induced obese mice.
abstract: We evaluated the effect of an oral administration of a plant-derived lactic acid bacterium, Pediococcus pentosaceus LP28 (LP28), on metabolic syndrome by using high fat diet-induced obese mice. The obese mice were divided into 2 groups and fed either a high fat or regular diet for 8 weeks. Each group was further divided into 3 groups, which took LP28, another plant-derived Lactobacillus plantarum SN13T (SN13T) or no lactic acid bacteria (LAB). The lean control mice were fed a regular diet without inducing obesity prior to the experiment. LP28 reduced body weight gain and liver lipid contents (triglyceride and cholesterol), in mice fed a high fat diet for 8 weeks (40%, 54%, and 70% less than those of the control group without LAB, and P = 0.018, P<0.001, and P = 0.021, respectively), whereas SN13T and the heat treated LP28 at 121°C for 15 min were ineffective. Abdominal visceral fat in the high fat diet mice fed with LP28 was also lower than that without LAB by 44%, although it was not significant but borderline (P = 0.076). The sizes of the adipocytes and the lipid droplets in the livers were obviously decreased. A real-time PCR analyses showed that lipid metabolism-related genes, such as CD36 (P = 0.013), SCD1 encoding stearoyl-CoA desaturase 1 (not significant but borderline, P = 0.066), and PPARγ encoding peroxisome proliferator-activated receptor gamma (P = 0.039), were down-regulated by taking LP28 continuously, when compared with those of the control group. In conclusion, LP28 may be a useful LAB strain for the prevention and reduction of the metabolic syndrome.
authors.affiliation: Department of Molecular Microbiology and Biotechnology, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan.
authors.forename: Xingrong
authors.initials: X
authors.lastname: Zhao
authors.forename: Fumiko
authors.initials: F
authors.lastname: Higashikawa
authors.forename: Masafumi
authors.initials: M
authors.lastname: Noda
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Kawamura
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Matoba
authors.forename: Takanori
authors.initials: T
authors.lastname: Kumagai
authors.forename: Masanori
authors.initials: M
authors.lastname: Sugiyama
mesh_terms: D017667:Adipocytes / Q000378:metabolism / Q000473:pathology; D000818:Animals; D048429:Cell Size; D059305:Diet, High-Fat / Q000009:adverse effects*; D004041:Dietary Fats; D019587:Dietary Supplements; D028481:Euphorbia / Q000382:microbiology*; D005234:Fatty Liver / Q000097:blood / Q000150:complications* / Q000517:prevention & control*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D008055:Lipids / Q000097:blood; D008099:Liver / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008820:Mice, Obese; D009765:Obesity / Q000097:blood / Q000150:complications* / Q000517:prevention & control*; D010376:Pediococcus / Q000378:metabolism*; D008517:Phytotherapy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015430:Weight Gain
chemical_list: D004041:Dietary Fats; D008055:Lipids; D012333:RNA, Messenger