title: Acinetobacter baumannii outer membrane protein A modulates the biogenesis of outer membrane vesicles.
abstract: Acinetobacter baumannii secretes outer membrane vesicles (OMVs) during both in vitro and in vivo growth, but the biogenesis mechanism by which A. baumannii produces OMVs remains undefined. Outer membrane protein A of A. baumannii (AbOmpA) is a major protein in the outer membrane and the C-terminus of AbOmpA interacts with diaminopimelate of peptidoglycan. This study investigated the role of AbOmpA in the biogenesis of A. baumannii OMVs. Quantitative and qualitative approaches were used to analyze OMV biogenesis in A. baumannii ATCC 19606T and an isogenic ΔAbOmpA mutant. OMV production was significantly increased in the ΔAbOmpA mutant compared to wild-type bacteria as demonstrated by quantitation of proteins and lipopolysaccharides (LPS) packaged in OMVs. LPS profiles prepared from OMVs from wild-type bacteria and the ΔAbOmpA mutant had identical patterns, but proteomic analysis showed different protein constituents in OMVs from wild-type bacteria compared to the ΔAbOmpA mutant. In conclusion, AbOmpA influences OMV biogenesis by controlling OMV production and protein composition.
authors.affiliation: Department of Microbiology, Kyungpook National University School of Medicine, Daegu, 700-422, Republic of Korea.
authors.forename: Dong Chan
authors.initials: DC
authors.lastname: Moon
authors.forename: Chul Hee
authors.initials: CH
authors.lastname: Choi
authors.forename: Jung Hwa
authors.initials: JH
authors.lastname: Lee
authors.forename: Chi-Won
authors.initials: CW
authors.lastname: Choi
authors.forename: Hye-Yeon
authors.initials: HY
authors.lastname: Kim
authors.forename: Jeong Soon
authors.initials: JS
authors.lastname: Park
authors.forename: Seung Il
authors.initials: SI
authors.lastname: Kim
authors.forename: Je Chul
authors.initials: JC
authors.lastname: Lee
mesh_terms: D040981:Acinetobacter baumannii / Q000235:genetics / Q000378:metabolism*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism; D001692:Biological Transport; D055354:Exosomes / Q000378:metabolism*; D055786:Gene Knockout Techniques; D008070:Lipopolysaccharides / Q000378:metabolism
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D008070:Lipopolysaccharides; C078167:OMPA outer membrane proteins