title: Gold nanoparticles/horseradish peroxidase encapsulated polyelectrolyte nanocapsule for signal amplification in Listeria monocytogenes detection.
abstract: Bioconjugate nanocapsules were fabricated by using polystyrene sulfonate (PSS) to encapsulate gold nanoparticles (AuNPs) bearing adsorbed horseradish peroxidase (HRP). The average size of nanocapsule was in a range 150-400 nm. The efficiency of the capsules to enhance signals in an immunoassay was demonstrated by using an enzyme linked immunosorbent assay (ELISA) to detect the food-borne pathogen -Listeria monocytogenes. The antibody adsorbed onto the PSS shell of the nanocapsules provided the recognition molecule. For a given quantity of antibody, the bioconjugate nanocapsules showed 30 times greater sensitivity and a shorter assay time (5 min) when compared to conventional ELISA using an HRP labelled antibody. This proof-of-concept encapsulation of HRP through PSS nanocapsules may pave the way for alternative signal enhancement strategies where sensitivity is a priority.
authors.affiliation: National Center for Genetic Engineering and Biotechnology, National Sciences and Technology Development Agency at King Mongkut's University of Technology Thonburi (Bangkhuntien), Bangkok, Thailand. sukunya.oae@biotec.or.th
authors.forename: Sukunya
authors.initials: S
authors.lastname: Oaew
authors.forename: Ratthaphol
authors.initials: R
authors.lastname: Charlermroj
authors.forename: Thitiporn
authors.initials: T
authors.lastname: Pattarakankul
authors.forename: Nitsara
authors.initials: N
authors.lastname: Karoonuthaisiri
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005516:Food Microbiology; D006046:Gold / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000737:chemistry; D006801:Humans; D008089:Listeria monocytogenes / Q000302:isolation & purification*; D053769:Nanocapsules / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry
chemical_list: D053769:Nanocapsules; D011137:Polystyrenes; C091521:horseradish peroxidase-coupled gold; C003321:polystyrene sulfonic acid; D006046:Gold; D006735:Horseradish Peroxidase