title: Extraction of intrinsic fluorescence from single fiber fluorescence measurements on a turbid medium.
abstract: This study utilizes Monte Carlo simulations of single fiber fluorescence to develop an empirical model that corrects for the influence of scattering and absorption on fluorescence intensity (F(SF)). The model expresses F(SF) in terms of the reduced scattering coefficient (μs') and absorption coefficient (μ(a)), each determined independently at excitation and emission wavelengths (λ(x) and λ(m)), and the fiber diameter (d(f)). This model returns accurate descriptions (mean residual <6%) of F(SF) across a biologically relevant range of μs' and μ(a) values and is insensitive to the form of the scattering phase function.
authors.affiliation: Center for Optical Diagnostics and Therapy, Department of Radiation Oncology, Erasmus Medical Center, Rotterdam 3000 CA, The Netherlands. s.kanick@erasmusmc.nl
authors.forename: Stephen C
authors.initials: SC
authors.lastname: Kanick
authors.forename: Dominic J
authors.initials: DJ
authors.lastname: Robinson
authors.forename: Henricus J C M
authors.initials: HJ
authors.lastname: Sterenborg
authors.forename: Arjen
authors.initials: A
authors.lastname: Amelink
mesh_terms: D000042:Absorption; D009010:Monte Carlo Method; D013050:Spectrometry, Fluorescence / Q000379:methods*