title: Effect of ascorbic acid on reactive oxygen species production in chemotherapy and hyperthermia in prostate cancer cells.
abstract: Cellular reactive oxygen species (ROS) production is increased by both temperature and anticancer drugs. Antioxidants are known to suppress ROS production while cancer patients may take them as dietary supplement during chemotherapy and hyperthermic therapy. We examined changes in ROS production in prostate cancer cells in the presence of various anticancer drugs and antioxidants at different temperatures. ROS production was increased with temperature in cancer cells, but not in normal cells; this increase was potently inhibited by ascorbic acid. ROS production was also increased in the presence of some anticancer drugs, such as vinblastine, but not by others. Dietary antioxidant supplements, such as β-carotene, showed variable effects. Ascorbic acid potently inhibited ROS production, even in the presence of anticancer drugs, while β-carotene showed no inhibition. Accordingly, our results suggest that cancer patients should carefully choose antioxidants during their cancer chemotherapy and/or hyperthermic therapy.
authors.affiliation: Department of Orthopaedic Surgery, Yokohama City University School of Medicine, 3-9 Fukuura, Kanazawaku, Yokohama 236-0004, Japan.
authors.forename: Hidenobu
authors.initials: H
authors.lastname: Fukumura
authors.forename: Motohiko
authors.initials: M
authors.lastname: Sato
authors.forename: Kyouhei
authors.initials: K
authors.lastname: Kezuka
authors.forename: Itaru
authors.initials: I
authors.lastname: Sato
authors.forename: Xianfeng
authors.initials: X
authors.lastname: Feng
authors.forename: Satoshi
authors.initials: S
authors.lastname: Okumura
authors.forename: Takayuki
authors.initials: T
authors.lastname: Fujita
authors.forename: Utako
authors.initials: U
authors.lastname: Yokoyama
authors.forename: Haruki
authors.initials: H
authors.lastname: Eguchi
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ishikawa
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Saito
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology*; D003911:Dextrans / Q000494:pharmacology; D006979:Hyperthermia, Induced; D058185:Magnetite Nanoparticles; D008297:Male; D011471:Prostatic Neoplasms / Q000188:drug therapy*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D003911:Dextrans; D058185:Magnetite Nanoparticles; D017382:Reactive Oxygen Species; C065507:ferumoxides; D001205:Ascorbic Acid