title: Effects of carbon sources and feeding strategies on heparosan production by Escherichia coli K5.
abstract: This work aimed to develop an optimal carbon source feeding strategy to achieve maximal production of heparosan as a precursor of bioengineered heparin by Escherichia coli K5. Glycerol gave higher heparosan titer and productivity compared to glucose. The maximum heparosan production (187 mg/L) and heparosan productivity (5.19 mg/L/h) in glycerol-defined medium were 26.4% higher than the heparosan production (148 mg/L) and heparosan productivity (4.11 mg/L/h) in glucose-defined medium. DO-stat feeding approach as compared to pH-stat feeding, exponential feeding, exponential combined with pH-stat feeding, and constant rate feeding gave the highest heparosan titer at 8.63 g/L, which was nine times that of batch culture. The obtained optimal glycerol feeding strategy may be useful for the scaling-up of microbial heparosan production.
authors.affiliation: Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, China.
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Liu
authors.forename: Long
authors.initials: L
authors.lastname: Liu
authors.forename: Jinghua
authors.initials: J
authors.lastname: Chen
authors.forename: Jianghua
authors.initials: J
authors.lastname: Li
authors.forename: Guocheng
authors.initials: G
authors.lastname: Du
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
mesh_terms: D002244:Carbon / Q000378:metabolism*; D003470:Culture Media; D004187:Disaccharides / Q000096:biosynthesis*; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D005285:Fermentation; D005990:Glycerol / Q000378:metabolism; D006863:Hydrogen-Ion Concentration
chemical_list: D003470:Culture Media; D004187:Disaccharides; C560363:heparosan; D002244:Carbon; D005990:Glycerol