title: Rice phospholipase A superfamily: organization, phylogenetic and expression analysis during abiotic stresses and development.
abstract: BACKGROUND
  Phospholipase A (PLA) is an important group of enzymes responsible for phospholipid hydrolysis in lipid signaling. PLAs have been implicated in abiotic stress signaling and developmental events in various plants species. Genome-wide analysis of PLA superfamily has been carried out in dicot plant Arabidopsis. A comprehensive genome-wide analysis of PLAs has not been presented yet in crop plant rice.
  
  
  RESULTS
  A comprehensive bioinformatics analysis identified a total of 31 PLA encoding genes in the rice genome, which are divided into three classes; phospholipase A(1) (PLA(1)), patatin like phospholipases (pPLA) and low molecular weight secretory phospholipase A(2) (sPLA(2)) based on their sequences and phylogeny. A subset of 10 rice PLAs exhibited chromosomal duplication, emphasizing the role of duplication in the expansion of this gene family in rice. Microarray expression profiling revealed a number of PLA members expressing differentially and significantly under abiotic stresses and reproductive development. Comparative expression analysis with Arabidopsis PLAs revealed a high degree of functional conservation between the orthologs in two plant species, which also indicated the vital role of PLAs in stress signaling and plant development across different plant species. Moreover, sub-cellular localization of a few candidates suggests their differential localization and functional role in the lipid signaling.
  
  
  CONCLUSIONS
  The comprehensive analysis and expression profiling would provide a critical platform for the functional characterization of the candidate PLA genes in crop plants.
authors.affiliation: Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi, India.
authors.forename: Amarjeet
authors.initials: A
authors.lastname: Singh
authors.forename: Vinay
authors.initials: V
authors.lastname: Baranwal
authors.forename: Alka
authors.initials: A
authors.lastname: Shankar
authors.forename: Poonam
authors.initials: P
authors.lastname: Kanwar
authors.forename: Rajeev
authors.initials: R
authors.lastname: Ranjan
authors.forename: Sandeep
authors.initials: S
authors.lastname: Yadav
authors.forename: Amita
authors.initials: A
authors.lastname: Pandey
authors.forename: Sanjay
authors.initials: S
authors.lastname: Kapoor
authors.forename: Girdhar K
authors.initials: GK
authors.lastname: Pandey
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics; D032461:Chromosomes, Plant / Q000235:genetics; D017124:Conserved Sequence / Q000235:genetics; D020440:Gene Duplication / Q000235:genetics; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D020131:Genes, Duplicate / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D012275:Oryza / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D010741:Phospholipases A / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D021381:Protein Transport; D015203:Reproducibility of Results; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013312:Stress, Physiological / Q000235:genetics*; D013347:Subcellular Fractions / Q000201:enzymology
chemical_list: D010940:Plant Proteins; D010741:Phospholipases A