title: Functional changes in human peripheral neutrophils in workers with different exposure to noxious agents.
abstract: Peripheral neutrophils (PMN) are a useful model to study cell activation and to evaluate the multiple stressors' interference with signalling pathways. This study aimed to evaluate the effects of different exposure to noxious agents on PMN activity by chemiluminescence assays. Two groups of office workers were recruited: peripheral whole blood cells and isolated PMNs were analyzed, performing intra- and extra-cellular chemiluminescence evaluation to study compartmental release of radical species. The exposed group shows a higher emission in resting whole blood chemiluminescence than in controls; the intracellular chemiluminescence emission in urban employees was increased, whereas the extracellular was not affected. After stimulation, the extracellular chemiluminescence was decreased in urban employees, whereas the intracellular emission did not show differences. We hypothesize that once stimulated, exposed subjects' PMNs respond to stimuli less than unexposed: PMNs could be considered indicators of chronic exposure to noxious agents.
authors.affiliation: Occupational Medicine, Hygiene and Industrial Toxicology Section, Department of Experimental Medicine, Second University of Naples, Via L. De Crecchio, 7, Naples, 80138, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Pedata
authors.forename: Elpidio Maria
authors.initials: EM
authors.lastname: Garzillo
authors.forename: Rosario
authors.initials: R
authors.lastname: Miranda
authors.forename: Andrea
authors.initials: A
authors.lastname: Antonucci
authors.forename: Daniela
authors.initials: D
authors.lastname: Feola
authors.forename: Nadia
authors.initials: N
authors.lastname: Miraglia
authors.forename: Nicola
authors.initials: N
authors.lastname: Sannolo
authors.forename: Monica
authors.initials: M
authors.lastname: Lamberti
mesh_terms: D000328:Adult; D000393:Air Pollutants / Q000633:toxicity; D016022:Case-Control Studies; D004032:Diet / Q000009:adverse effects; D004574:Electromagnetic Fields / Q000009:adverse effects; D005609:Free Radicals / Q000378:metabolism*; D006801:Humans; D008019:Life Style; D008163:Luminescent Measurements; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000166:cytology*; D016273:Occupational Exposure; D012307:Risk Factors; D013315:Stress, Psychological
chemical_list: D000393:Air Pollutants; D005609:Free Radicals