title: Snake venomics of Macrovipera mauritanica from Morocco, and assessment of the para-specific immunoreactivity of an experimental monospecific and a commercial antivenoms.
abstract: Proteomic analysis of the venom of the medically relevant snake Macrovipera mauritanica from Morocco revealed a complex proteome composed of at least 45 toxins from 9 protein families targeting the hemostatic system of the prey or victim. The toxin profile of Moroccan M. mauritanica displays great similarity, but also worth noting departures, with the previously reported venom proteome of M. lebetina from Tunisia. Despite fine compositional differences between these Macrovipera taxa, their overall venom phenotypes explain the clinical picture observed in M. mauritanica and M. lebetina envenomings. However, M. mauritanica venom also contains significant amounts of orphan molecules whose presence in the venom seems to be difficult to rationalize in the context of a predator-prey arms race. The paraspecific immunoreactivity of an experimental monospecific (M. mauritanica) antivenom and a commercial bivalent antivenom, anti-C. cerastes and anti-M. lebetina, against the venoms of Moroccan M. mauritanica and Tunisian M. lebetina, was also investigated through an affinity chromatography-based antivenomics approach. Both antivenoms very efficiently immunodepleted homologous venom toxins and displayed a high degree of paraspecificity, suggesting the clinical utility of the two antivenoms for treating bites of both M. mauritanica or M. lebetina.
authors.affiliation: Venoms and Toxins Laboratory, Pasteur Institute of Morocco, 1. Place Louis Pasteur, 20100-Casablanca, Morocco.
authors.forename: Bouchra
authors.initials: B
authors.lastname: Makran
authors.forename: Laila
authors.initials: L
authors.lastname: Fahmi
authors.forename: Davinia
authors.initials: D
authors.lastname: Pla
authors.forename: Libia
authors.initials: L
authors.lastname: Sanz
authors.forename: Naoual
authors.initials: N
authors.lastname: Oukkache
authors.forename: Mustapha
authors.initials: M
authors.lastname: Lkhider
authors.forename: Noreddine
authors.initials: N
authors.lastname: Ghalim
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Calvete
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000378:metabolism; D000915:Antibody Affinity; D000918:Antibody Specificity; D000997:Antivenins / Q000276:immunology* / Q000378:metabolism; D003132:Commerce; D003435:Crotalid Venoms / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D015507:Drugs, Investigational; D005843:Geography; D047468:Immunoprecipitation / Q000379:methods*; D008877:Middle East; D009018:Morocco; D020543:Proteome / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D012911:Snakes / Q000378:metabolism*; D014416:Tunisia
chemical_list: D000911:Antibodies, Monoclonal; D000997:Antivenins; D003435:Crotalid Venoms; D015507:Drugs, Investigational; D020543:Proteome