title: Identification of Arcanobacterium (Trueperella) abortisuis, a novel species of veterinary importance, by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS).
abstract: In the present study A. (T.) abortisuis isolated from pigs and bovines could be reliably identified by determination of phenotypic properties, genotypically by polymerase chain reaction with the help of A. (T.) abortisuis 16s-23S rDNA intergenic spacer region specific oligonucleotide primer and by Matrix-Assisted Laser Desorption Ionization-Time Of Flight mass spectrometry (MALDI-TOF MS). The latter appeared to be a promising tool for fast and cost effective identification of this species and might help to elucidate the role A. (T.) abortisuis plays in infections of pigs, bovines, possibly other animals or humans.
authors.affiliation: Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Giessen, Giessen, Germany.
authors.forename: Muaz
authors.initials: M
authors.lastname: Hijazin
authors.forename: Hivda
authors.initials: H
authors.lastname: Ulbegi-Mohyla
authors.forename: Jörg
authors.initials: J
authors.lastname: Alber
authors.forename: Christoph
authors.initials: C
authors.lastname: Lämmler
authors.forename: Abdulwahed Ahmed
authors.initials: AA
authors.lastname: Hassan
authors.forename: Markus
authors.initials: M
authors.lastname: Timke
authors.forename: Markus
authors.initials: M
authors.lastname: Kostrzewa
authors.forename: Ellen
authors.initials: E
authors.lastname: Prenger-Berninghoff
authors.forename: Reinhard
authors.initials: R
authors.lastname: Weiss
authors.forename: Michael
authors.initials: M
authors.lastname: Zschöck
mesh_terms: D000193:Actinomycetales Infections / Q000382:microbiology / Q000662:veterinary*; D000818:Animals; D055667:Arcanobacterium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques / Q000379:methods*; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005260:Female; D008297:Male; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
chemical_list: D021903:DNA, Ribosomal Spacer; D012336:RNA, Ribosomal, 16S