title: Proteomic analysis of early seed development in Pinus massoniana L.
abstract: Understanding seed development is important for large-scale propagation and germplasm conservation for the Masson pine. We undertook a proteomic analysis of Masson pine seeds during the early stages of embryogenesis. Two-dimensional difference gel electrophoresis (2D DIGE) was used to quantify the differences in protein expression during early seed development. Using electrospray ionization mass spectrometry/mass spectrometry, we identified proteins from 43 gel spots that had been excised from preparative "pick" gels. Proteins involved in carbon metabolism were identified and were predominantly expressed at higher levels during the cleavage polyembryony and columnar embryo stages. Functional annotation of one seed protein revealed it involvement in programmed cell death and translation of selective mRNAs, which may play an important role in subordinate embryo elimination and suspensor degeneration in polyembryonic seed gymnosperms. Other identified proteins were associated with protein folding, nitrogen metabolism, disease/defense response, and protein storage, synthesis and stabilization. The comprehensive protein expression profiles generated by this study will provide new insights into the complex developmental process of seed development in Masson pine.
authors.affiliation: Key Laboratory of Forest Genetics and Biotechnology, Nanjing Forestry University, Ministry of Education, Nanjing 210037, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhen
authors.forename: Zhen-Zhou
authors.initials: ZZ
authors.lastname: Zhao
authors.forename: Ren-Hua
authors.initials: RH
authors.lastname: Zheng
authors.forename: Jisen
authors.initials: J
authors.lastname: Shi
mesh_terms: D002244:Carbon / Q000378:metabolism; D016923:Cell Death; D028223:Pinus / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D014176:Protein Biosynthesis; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D012333:RNA, Messenger; D012639:Seeds / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization; D057825:Two-Dimensional Difference Gel Electrophoresis
chemical_list: D010940:Plant Proteins; D020543:Proteome; D012333:RNA, Messenger; D002244:Carbon