title: Micro-array profiling exhibits remarkable intra-individual stability of human platelet micro-RNA.
abstract: Platelets play an important role in haemostasis and thrombus formation. Latest research identified platelets harbouring so called microRNAs (miRNA). MiRNAs are short single-stranded RNAs modulating gene expression by targeting mRNAs. Limited data exist on inter-individual variability of platelet miRNA profile while no data are available on intra-individual variability. We assessed platelet miRNA profile in five volunteers at five time points over a time course of 10 days; 24 hours prior to the last blood sampling, subjects took 500 mg acetylsalicylic acid (ASA). Platelet miRNA was isolated from leucocyte-depleted platelet-rich plasma, and miRNA array-analysis was performed. Temporal patterns and ASA effect were explored by a linear mixed effects model for each miRNA. For the 20 most abundantly expressed platelet miRNAs, target gene search was performed and an annotation network was created. MiRNA expression profiling of 1,281 human miRNAs revealed relevant expression of 221 miRNAs consistently expressed in all samples at all time points. Correlation of platelet miRNA ranks was highly significant to other studies. Global distribution of miRNA expression was relatively similar in all subjects. No miRNA exhibited a significant effect of time at level 0.05. After 24 hours, no significant effect of ASA was found. Concerning functional implications of the 20 most abundantly expressed miRNAs, we found six functional themes. In conclusion, platelet miRNA profile is remarkably stable over the time period studied. Single-point analysis of platelet miRNA profile is reasonable when inter-individual differences are studied. The functional annotation network points toward extra-platelet effects of platelet miRNAs.
authors.affiliation: Bernd L. Fiebich, Department of Psychiatry and Psychotherapy, University of Freiburg Medical School, Hauptstr. 5, D-79014 Freiburg, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Stratz
authors.forename: T G
authors.initials: TG
authors.lastname: Nührenberg
authors.forename: H
authors.initials: H
authors.lastname: Binder
authors.forename: C M
authors.initials: CM
authors.lastname: Valina
authors.forename: D
authors.initials: D
authors.lastname: Trenk
authors.forename: W
authors.initials: W
authors.lastname: Hochholzer
authors.forename: F J
authors.initials: FJ
authors.lastname: Neumann
authors.forename: B L
authors.initials: BL
authors.lastname: Fiebich
mesh_terms: D000328:Adult; D001241:Aspirin / Q000494:pharmacology; D001792:Blood Platelets / Q000166:cytology*; D019295:Computational Biology / Q000379:methods; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D007962:Leukocytes / Q000166:cytology; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D008954:Models, Biological; D020411:Oligonucleotide Array Sequence Analysis; D010974:Platelet Aggregation; D015203:Reproducibility of Results; D013048:Specimen Handling / Q000379:methods; D013997:Time Factors
chemical_list: D035683:MicroRNAs; D001241:Aspirin