title: 8-Vinylguanine nucleo amino acid: a fluorescent PNA building block.
abstract: Attachment of a vinyl group at guanine position 8 provides fluorescent properties of the nucleobase. Therefore, 8-vinylguanine was introduced as a 2-aminoethylglycine peptide nucleic acid (PNA) building block. Incorporation of the guanine analog in short PNA sequences by Fmoc solid phase peptide synthesis allowed the differentiation between hybridization states of specific double strands with DNA, RNA, and PNA as well as quadruplex forming RNA/PNA oligomers based on fluorescence intensity.
authors.affiliation: Institut für Organische und Biomolekulare Chemie, Georg-August-Universität Göttingen, Tammannstrasse 2, 37077 Göttingen, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Müllar
authors.forename: Julian
authors.initials: J
authors.lastname: Strohmeier
authors.forename: Ulf
authors.initials: U
authors.lastname: Diederichsen
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D004247:DNA / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D006147:Guanine / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis / Q000737:chemistry*; D012313:RNA / Q000737:chemistry
chemical_list: D000596:Amino Acids; D005456:Fluorescent Dyes; D020135:Peptide Nucleic Acids; D006147:Guanine; D012313:RNA; D004247:DNA