title: Bafibrinase: A non-toxic, non-hemorrhagic, direct-acting fibrinolytic serine protease from Bacillus sp. strain AS-S20-I exhibits in vivo anticoagulant activity and thrombolytic potency.
abstract: A non-toxic, direct-acting fibrinolytic serine protease (Bafibrinase) demonstrating thrombolytic and anticoagulant properties was purified from Bacillus sp. strain AS-S20-I. Bafibrinase was monomeric, with a molecular mass of 32.3 kDa. The peptide mass fingerprinting of Bafibrinase revealed only 8.3% sequence coverage, suggesting it was a novel fibrinolytic enzyme. However, two of the tryptic digested de novo peptide sequences of Bafibrinase demonstrated good similarity with endopeptidases possessing serine in their catalytic triad. Further, catalytic activity of Bafibrinase was inhibited by serine protease inhibitor reinforcing this is a subtilisin-like serine protease. The apparent K(m) and V(max) values of Bafibrinase towards fibrin were determined as 0.24 μM and 2.8 μmol/min, respectively. It showed a K(m) value of 0.139 mM towards a chromogenic substrate for plasmin (D-Val-Leu-Lys-p-Nitroanilide dihydrochloride) and optimum activity at physiological conditions (37 °C and pH 7.4). Based on the cleavage pattern of fibrin and fibrinogen, Bafibrinase may be classified as an α,β-fibrinogenase. Bafibrinase could not degrade collagen and was non-cytotoxic to HT29 cells or mammalian erythrocytes. Further, Bafibrinase at a dose of 2 mg/kg was devoid of toxicity as well as hemorrhagic activity on BALB/c mouse model, supporting its suitability for the development of a better and safer thrombolytic drug. Bafibrinase was also superior to human plasmin in degrading in vitro thrombus. The in vivo anticoagulant nature of Bafibrinase is being explored for the treatment and prevention of thrombosis and other cardiovascular diseases.
authors.affiliation: Microbial Biotechnology and Protein Research Laboratory, Department of Molecular Biology and Biotechnology, School of Science and Technology, Tezpur University, Tezpur 784 028, Assam, India. akm@tezu.ernet.in
authors.forename: Ashis K
authors.initials: AK
authors.lastname: Mukherjee
authors.forename: Sudhir K
authors.initials: SK
authors.lastname: Rai
authors.forename: Rupamoni
authors.initials: R
authors.lastname: Thakur
authors.forename: Pronobesh
authors.initials: P
authors.lastname: Chattopadhyay
authors.forename: Santosh K
authors.initials: SK
authors.lastname: Kar
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology*; D001407:Bacillus / Q000201:enzymology; D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology*; D005337:Fibrin / Q000378:metabolism; D005342:Fibrinolysis / Q000187:drug effects; D005343:Fibrinolytic Agents / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D012697:Serine Endopeptidases / Q000378:metabolism*; D057057:Serine Proteases / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology*; D015912:Thrombolytic Therapy
chemical_list: D000925:Anticoagulants; D001426:Bacterial Proteins; D005343:Fibrinolytic Agents; D005337:Fibrin; D057057:Serine Proteases; C574622:bafibrinase protein, Bacillus; D012697:Serine Endopeptidases