title: Microarray analysis of mediastinal lymph node of pigs naturally affected by postweaning multisystemic wasting syndrome.
abstract: Postweaning multisystemic wasting syndrome (PMWS) is one of the pig diseases with major economic impact worldwide. Clinical, pathologic and some immunologic aspects of this disease are relatively well-known, but the molecular mechanisms underlying the pathogenic mechanisms of the disease are still poorly understood. The objective of the present study was to investigate the global transcriptome changes in the mediastinal lymph nodes from pigs naturally affected by PMWS, as well as healthy counterparts, using the Affymetrix Porcine Genechip(®). From 366 transcripts showing significant differential abundance in the PMWS group of pigs relative to healthy animals, 229 showed higher and 137 lower abundance. A relative increased abundance of mRNAs coded by a large set of genes involved in the inflammatory responses (e.g. cytokines, acute phase proteins, and respiratory burst) was observed in PMWS affected pigs. The Gpnmb and Lgals3 genes, which have antagonistic functions in regulation of inflammatory processes, showed high mRNA levels in diseased pigs. The complement system was altered by PMWS, notably by the lower levels of Cr1 mRNA, which might favour both complement deposition and secondary infections by impairing phagocytosis. Decreased mRNA abundance of several genes involved in lymphocyte activation/differentiation, such as Cd79b, Cd19, Cd21 and MybL1, and the high level of Vsig4 mRNA, which can compromise the activation of residing T-cells, pointed towards a defective adaptive immunity. This is the first study on gene expression in pigs naturally affected by PMWS. The present results allowed identifying potential mechanisms underlying the inflammation and lymphocyte depletion in lymphoid tissues by complement mediated damage and immunosuppression, which are key features of PMWS.
authors.affiliation: Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona Bellaterra, Bellaterra, Barcelona, Spain.
authors.forename: L T
authors.initials: LT
authors.lastname: Fernandes
authors.forename: A
authors.initials: A
authors.lastname: Tomás
authors.forename: A
authors.initials: A
authors.lastname: Bensaid
authors.forename: M
authors.initials: M
authors.lastname: Sibila
authors.forename: A
authors.initials: A
authors.lastname: Sánchez
authors.forename: J
authors.initials: J
authors.lastname: Segalés
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling; D007249:Inflammation / Q000276:immunology / Q000473:pathology; D008198:Lymph Nodes / Q000276:immunology / Q000473:pathology*; D008482:Mediastinum / Q000473:pathology*; D046228:Microarray Analysis; D053570:Porcine Postweaning Multisystemic Wasting Syndrome / Q000276:immunology / Q000473:pathology*; D013552:Swine