title: [The short duration functional electrical stimuli do not cause mRNA expression change of K(ATP) channel and acute inflammation cytokine in recurrent laryngeal nerve].
abstract: OBJECTIVE
  To investigate whether the inflammation response and K(ATP) channel damage happened because of acute injury after functional electrical stimuli (FES) act on the recurrent laryngeal nerve (RLN).
  
  
  METHODS
  Thirty rabbits were divided into 3 groups by 10 rabbits for each group. The unilateral RNL were stimulated by FES with cuff electrode in first group. There was electrode on the RNL without FES in second group. The third group was normal control. The electrical generator and isolation unit provide electrical stimulation to the RLN at 50 Hz, with 4 mA current, for 2ms pulse duration. The total train duration was 5 seconds (2 seconds on; 3 seconds off). The RLN were collected in 1 hour after 3 hours electrical stimuli. We measured mRNA expression of injury acute response inflammation cytokine IL-10, TGF-beta1, TNF-alpha and K(ATP) Subunit protein Kir6.1, Kir6.2, SUR2AB, using real-time reverse transcribed-polymerase chain reaction. PCR products were verified by electrophoresis in agarose gels. We investigated the vocal synchronic adductive movement with RLN FES by endoscopy. We investigated the nerve local RNL morphological burn. Data were analyzed with the One-way ANOVA analysis of SPSS version 13.0 for Windows.
  
  
  RESULTS
  There were no significance difference among three groups in mRNA expression of inflammation cytokines IL-10, TGF-beta1, TNF-alpha and K(ATP) channel protein Kir6.1, Kir6., SUR2AB. We did not find the nerve morphological burn in all rabbits RLN. The vocal synchronic adductive movement were normal during stimuli time.
  
  
  CONCLUSIONS
  The short duration functional electrical stimuli do not cause mRNA expression change of K(ATP) channel and acute inflammation cytokine in recurrent laryngeal nerve.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Guangdong Academy of Medical Sciences & Guangdong General Hospital, Guangzhou, 510080, China.
authors.forename: Xuwen
authors.initials: X
authors.lastname: Zhang
authors.forename: Pingjiang
authors.initials: P
authors.lastname: Ge
authors.forename: Yong
authors.initials: Y
authors.lastname: Cui
authors.forename: Jie
authors.initials: J
authors.lastname: Jiang
authors.forename: Jiandong
authors.initials: J
authors.lastname: Zhan
authors.forename: Qianhui
authors.initials: Q
authors.lastname: Qiu
authors.forename: Shaohua
authors.initials: S
authors.lastname: Chen
mesh_terms: D000818:Animals; D016207:Cytokines / Q000378:metabolism*; D004558:Electric Stimulation; D007249:Inflammation; D016753:Interleukin-10 / Q000378:metabolism; D054086:KATP Channels / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D012009:Recurrent Laryngeal Nerve / Q000378:metabolism* / Q000502:physiology; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D016207:Cytokines; D054086:KATP Channels; D012333:RNA, Messenger; D053773:Transforming Growth Factor beta1; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10