title: Calcium oxalate monohydrate crystals stimulate monocyte chemoattractant             protein-1 and transforming growth factor β1 expression in human renal epithelial             cells.
abstract: Crystal-cell interactions play a key role in the formation of kidney stones. Few studies have referred to the role of monocyte chemoattractant protein-1 (MCP-1) and transforming growth factor β1 (TGFβ1) in kidney stone formation. Recently, a genome-wide analysis of genes related to kidney stone formation and eliminiation in mice indicated that MCP-1 and TGFβ1 are involved in nephrolithiasis. In this study, in order to verify whether MCP-1 and TGFβ1 are involved in the process of crystal-cell interactions in vitro, we observed the effects of calcium oxalate monohydrate (COM) on MCP-1 and TGFβ1 expression in cultured HK-2 cells. HK-2 cells were treated with different concentrations of COM, and a group of untreated cells served as the control. The expression of MCP-1 and TGFβ1 was detected by western blot analysis after treatments with different COM concentrations (300, 500, 700 and 900 µg/ml) for different times (3, 6, 12 and 24 h). We found that the expression of MCP-1 was upregulated by COM treatment in a dose-dependent manner, and was increased initially at the first 6 h of treatment, then slightly decreased over time. Also, COM treatment resulted in a dose-dependent increase in TGFβ1 expression, and the expression levels peaked at 12 h. This study demonstrates that COM stimulates the expression of MCP-1 and TGFβ1 in renal epithelial cells.
authors.affiliation: Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, PR China.
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Liu
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Shaogang
authors.initials: S
authors.lastname: Wang
authors.forename: Weimin
authors.initials: W
authors.lastname: Yang
authors.forename: Jihong
authors.initials: J
authors.lastname: Liu
authors.forename: Zhangqun
authors.initials: Z
authors.lastname: Ye
mesh_terms: D000818:Animals; D002129:Calcium Oxalate / Q000494:pharmacology*; D002460:Cell Line; D018932:Chemokine CCL2 / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007668:Kidney / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D053040:Nephrolithiasis / Q000378:metabolism / Q000473:pathology; D013997:Time Factors; D053773:Transforming Growth Factor beta1 / Q000096:biosynthesis*; D015854:Up-Regulation / Q000187:drug effects*
chemical_list: C111835:CCL2 protein, human; D018932:Chemokine CCL2; D053773:Transforming Growth Factor beta1; D002129:Calcium Oxalate