title: Knockdown of Sec6 improves cell-cell adhesion by increasing α-E-catenin in oral cancer cells.
abstract: The Sec6/8 complex is essential for specific exocytic sites on the plasma membrane and contributes to membrane growth in mammalian cells. In Madin-Darby canine kidney (MDCK) cells, E-cadherin and nectin-based adhesion complexes recruit the Sec6/8 complex to intercellular contacts. However, in cancer cells, the relationship between the Sec6/8 complex and the cell-cell adhesion proteins remains obscure. We demonstrate that the expression of α-E-catenin is increased by Sec6 siRNAs, and E-cadherin and β-catenin localize mainly at the cell-cell contact region in HSC3 cells, which were transfected with Sec6 siRNA.
authors.affiliation: Department of Anatomy and Cell Biology, School of Medicine, Faculty of Medicine, Yamagata University, Yamagata, Japan. ttanaka@med.id.yamagata-u.ac.jp
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Tanaka
authors.forename: Mituyoshi
authors.initials: M
authors.lastname: Iino
authors.forename: Kaoru
authors.initials: K
authors.lastname: Goto
mesh_terms: D000818:Animals; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002448:Cell Adhesion / Q000502:physiology*; D015815:Cell Adhesion Molecules / Q000378:metabolism; D045744:Cell Line, Tumor; D006801:Humans; D009062:Mouth Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D000075983:Nectins; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism*; D033921:Vesicular Transport Proteins / Q000235:genetics / Q000378:metabolism*; D051178:alpha Catenin / Q000235:genetics / Q000378:metabolism*; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism
chemical_list: D015820:Cadherins; D015815:Cell Adhesion Molecules; C490685:EXOC3 protein, human; D000075983:Nectins; D034741:RNA, Small Interfering; D033921:Vesicular Transport Proteins; D051178:alpha Catenin; D051176:beta Catenin