title: Effectiveness of two disinfectant solutions and microwave irradiation in disinfecting complete dentures contaminated with methicillin-resistant Staphylococcus aureus.
abstract: BACKGROUND
  Methicillin-resistant Staphylococcus aureus (MRSA) biofilm on dentures can be aspirated, thus causing infections such as aspiration pneumonia. The authors evaluated the efficacy of two disinfectant solutions and microwave irradiation in disinfecting complete dentures contaminated with MRSA.
  
  
  METHODS
  The authors contaminated 36 simulated complete dentures with MRSA and divided them into four equal groups: a positive control group consisting of dentures that were not disinfected; a group that soaked in 1 percent sodium hypochlorite for 10 minutes; a group that soaked in 2 percent chlorhexidine gluconate for 10 minutes; and a group that underwent microwave irradiation at 650 watts for three minutes. The authors quantified colony counts and evaluated the long-term effectiveness of disinfection.
  
  
  RESULTS
  All dentures from the control group showed substantial microbial growth on the plates (6.24 log(10) colony-forming units per milliliter). The authors observed no evidence of microbial growth on plates of any disinfected dentures. After seven days' incubation, the authors observed broth turbidity in all beakers containing the dentures disinfected with 1 percent sodium hypochlorite.
  
  
  CONCLUSIONS
  Soaking in chlorhexidine gluconate solution and microwave irradiation resulted in complete disinfection of all dentures contaminated with MRSA in both the short and the long term. Soaking in sodium hypochlorite solution was effective only as a short-term disinfectant.
  
  
  CONCLUSIONS
  Microwave irradiation and 2 percent chlorhexidine gluconate may have a disinfective application in dental offices and institutions in which denture wearers are treated, thus improving the longevity and quality of life of patients and reducing the burden of disease caused by MRSA.
authors.affiliation: Department of Dental Materials and Prosthodontics, Araraquara Dental School, UNESP-Universidade Estadual Paulista, São Paulo, Brazil.
authors.forename: Karen Tereza
authors.initials: KT
authors.lastname: Altieri
authors.forename: Paula Volpato
authors.initials: PV
authors.lastname: Sanitá
authors.forename: Ana Lucia
authors.initials: AL
authors.lastname: Machado
authors.forename: Eunice Teresinha
authors.initials: ET
authors.lastname: Giampaolo
authors.forename: Ana Cláudia
authors.initials: AC
authors.lastname: Pavarina
authors.forename: Carlos Eduardo
authors.initials: CE
authors.lastname: Vergani
mesh_terms: D002710:Chlorhexidine / Q000031:analogs & derivatives / Q000494:pharmacology; D015169:Colony Count, Microbial; D019551:Dental Disinfectants / Q000494:pharmacology*; D003824:Denture, Complete / Q000382:microbiology*; D004203:Disinfection / Q000379:methods*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects* / Q000528:radiation effects*; D008872:Microwaves; D012973:Sodium Hypochlorite / Q000494:pharmacology
chemical_list: D019551:Dental Disinfectants; D012973:Sodium Hypochlorite; C010882:chlorhexidine gluconate; D002710:Chlorhexidine