title: Interrogating signaling nodes involved in cellular transformations using kinase activity probes.
abstract: Protein kinases catalyze protein phosphorylation and thereby control the flow of information through signaling cascades. Currently available methods for concomitant assessment of the enzymatic activities of multiple kinases in complex biological samples rely on indirect proxies for enzymatic activity, such as posttranslational modifications to protein kinases. Our laboratories have recently described a method for directly quantifying the enzymatic activity of kinases in unfractionated cell lysates using substrates containing a phosphorylation-sensitive unnatural amino acid termed CSox, which can be monitored using fluorescence. Here, we demonstrate the utility of this method using a probe set encompassing p38α, MK2, ERK1/2, Akt, and PKA. This panel of chemosensors provides activity measurements of individual kinases in a model of skeletal muscle differentiation and can be readily used to generate individualized kinase activity profiles for tissue samples from clinical cancer patients.
authors.affiliation: Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: Cliff I
authors.initials: CI
authors.lastname: Stains
authors.forename: Nathan C
authors.initials: NC
authors.lastname: Tedford
authors.forename: Traci C
authors.initials: TC
authors.lastname: Walkup
authors.forename: Elvedin
authors.initials: E
authors.lastname: Luković
authors.forename: Brenda N
authors.initials: BN
authors.lastname: Goguen
authors.forename: Linda G
authors.initials: LG
authors.lastname: Griffith
authors.forename: Douglas A
authors.initials: DA
authors.lastname: Lauffenburger
authors.forename: Barbara
authors.initials: B
authors.lastname: Imperiali
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D019073:HT29 Cells; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D006912:Hydroxyquinolines / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D051379:Mice; D018482:Muscle, Skeletal / Q000166:cytology / Q000201:enzymology; D009369:Neoplasms / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D015398:Signal Transduction; D013379:Substrate Specificity; D013449:Sulfonamides / Q000737:chemistry / Q000378:metabolism*
chemical_list: D006912:Hydroxyquinolines; D013449:Sulfonamides; D011494:Protein Kinases