title: Improved biomass saccharification by Trichoderma reesei through heterologous expression of lacA gene from Trametes sp. AH28-2.
abstract: The Trametes sp. AH28-2 laccase gene lacA fused to cellobiohydrolase I signal peptide coding sequence was heterologously expressed in T. reesei. The lacA cDNA was under the control of the Aspergillus nidulans glyceraldehyde-3-phosphate dehydrogenase promoter. Native PAGE analysis indicated that two transformants, L8 and L38, were able to secrete recombinant laccase A, and their laccase activities corresponding to ABTS oxidation reached 3.62 IUml(-1) and 1.50 IUml(-1) respectively. Most of the characteristics of the recombinant laccase were similar to those of the native enzyme. Reducing sugar yields of L8 and L38 obtained from saccharification of corn residue by crude enzyme increased by 31.3% and 71.6% respectively compared to the host strain. These results indicated that the engineering strains developed in this work could be potentially used for laccase production and tailoring cellulase properties with laccase proteins through genetic manipulation would be a feasible strategy to improve saccharification efficiency of biomass by cellulase preparation.
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China.
authors.forename: Jiwei
authors.initials: J
authors.lastname: Zhang
authors.forename: Yinbo
authors.initials: Y
authors.lastname: Qu
authors.forename: Peng
authors.initials: P
authors.lastname: Xiao
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Wang
authors.forename: Tianhong
authors.initials: T
authors.lastname: Wang
authors.forename: Feng
authors.initials: F
authors.lastname: He
mesh_terms: D001233:Aspergillus nidulans / Q000235:genetics / Q000378:metabolism; D001673:Biodegradation, Environmental; D057005:Bioengineering; D018533:Biomass; D002480:Cellulase / Q000378:metabolism; D043366:Cellulose 1,4-beta-Cellobiosidase / Q000235:genetics / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D042845:Laccase / Q000378:metabolism*; D063347:Native Polyacrylamide Gel Electrophoresis; D055454:Trametes / Q000235:genetics* / Q000502:physiology; D014242:Trichoderma / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D042845:Laccase; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D002480:Cellulase; D043366:Cellulose 1,4-beta-Cellobiosidase