title: High transformation efficiency of Bacillus subtilis with integrative DNA using glycine betaine as osmoprotectant.
abstract: Electroporation is an important approach for genetic engineering experiments allowing for introduction of foreign DNA in a selected host. Here, we describe for the first time the use of glycine betaine as an osmoprotectant for electroporation of gram-positive bacteria Bacillus subtilis. High electroporation efficiency (up to 5×10(5) cfu/μg) was obtained using 7.5% glycine betaine. The new method improved the transformation efficiency of B. subtilis with linear integrative DNA nearly 700-fold compared with existing Bacillus transformation techniques.
authors.affiliation: Département de Chimie-Biologie, Université du Québec à Trois-Rivières, Trois-Rivières, Québec, Canada.
authors.forename: Fatma
authors.initials: F
authors.lastname: Meddeb-Mouelhi
authors.forename: Carlos
authors.initials: C
authors.lastname: Dulcey
authors.forename: Marc
authors.initials: M
authors.lastname: Beauregard
mesh_terms: D001412:Bacillus subtilis / Q000187:drug effects / Q000235:genetics*; D001622:Betaine / Q000494:pharmacology*; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000235:genetics; D018274:Electroporation / Q000379:methods*; D009994:Osmolar Concentration; D009997:Osmotic Pressure / Q000187:drug effects; D010957:Plasmids / Q000737:chemistry / Q000235:genetics; D014169:Transformation, Bacterial
chemical_list: D001622:Betaine; D004247:DNA