title: Compaction enhances extracellular matrix content and mechanical properties of tissue-engineered cartilaginous constructs.
abstract: Many cell-based tissue-engineered cartilaginous constructs are mechanically softer than native tissue and have low content and abnormal proportions of extracellular matrix (ECM) constituents. We hypothesized that the load-bearing mechanical properties of cartilaginous constructs improve with the inclusion of collagen (COL) and proteoglycan (PG) during assembly. The objectives of this work were to determine (1) the effect of addition of PG, COL, or COL+PG on compressive properties of 2% agarose constructs and (2) the ability of mechanical compaction to concentrate matrix content and improve the compressive properties of such constructs. The inclusion of COL+PG improved the compressive properties of hydrogel constructs compared with PG or COL alone. Mechanical compaction increased the PG and COL concentrations in and compressive stiffness of the constructs. Chondrocytes included in the constructs maintained high viability after compaction. These results support the concepts that the assembly of cartilaginous constructs with COL+PG and application of mechanical compaction enhance the ECM content and compressive properties of engineered cartilaginous constructs.
authors.affiliation: Department of Bioengineering, University of California-San Diego, La Jolla, California, USA.
authors.forename: EunHee
authors.initials: EH
authors.lastname: Han
authors.forename: Chenghao
authors.initials: C
authors.lastname: Ge
authors.forename: Albert C
authors.initials: AC
authors.lastname: Chen
authors.forename: Barbara L
authors.initials: BL
authors.lastname: Schumacher
authors.forename: Robert L
authors.initials: RL
authors.lastname: Sah
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena / Q000502:physiology; D002356:Cartilage / Q000502:physiology*; D002417:Cattle; D002470:Cell Survival; D019902:Chondrocytes / Q000166:cytology; D024043:Collagen Type II / Q000378:metabolism; D019245:Compressive Strength / Q000502:physiology; D005109:Extracellular Matrix / Q000378:metabolism*; D006025:Glycosaminoglycans / Q000378:metabolism; D008422:Materials Testing; D008954:Models, Biological; D013194:Staining and Labeling; D013314:Stress, Mechanical; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D024043:Collagen Type II; D006025:Glycosaminoglycans; C013786:A73025