title: The synthesis of 2'-methylseleno adenosine and guanosine 5'-triphosphates.
abstract: Modified nucleoside triphosphates (NTPs) represent powerful building blocks to generate nucleic acids with novel properties by enzymatic synthesis. We have recently demonstrated the access to 2'-SeCH(3)-uridine and 2'-SeCH(3)-cytidine derivatized RNAs for applications in RNA crystallography, using the corresponding nucleoside triphosphates and distinct mutants of T7 RNA polymerase. In the present note, we introduce the chemical synthesis of the novel 2'-methylseleno-2'-deoxyadenosine and -guanosine 5'-triphosphates (2'-SeCH(3)-ATP and 2'-SeCH(3)-GTP) that represent further candidates for the enzymatic RNA synthesis with engineered RNA polymerases.
authors.affiliation: Institute of Organic Chemistry and Center for Molecular Biosciences CMBI, University of Innsbruck, 6020 Innsbruck, Austria.
authors.forename: Tobias
authors.initials: T
authors.lastname: Santner
authors.forename: Vanessa
authors.initials: V
authors.lastname: Siegmund
authors.forename: Andreas
authors.initials: A
authors.lastname: Marx
authors.forename: Ronald
authors.initials: R
authors.lastname: Micura
mesh_terms: D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D012321:DNA-Directed RNA Polymerases / Q000235:genetics / Q000378:metabolism; D003838:Deoxyadenine Nucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D003848:Deoxyguanine Nucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D006160:Guanosine Triphosphate / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D009154:Mutation; D016566:Organoselenium Compounds; D012643:Selenium / Q000737:chemistry*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: C573391:2'-methylseleno-2'-deoxyadenosine-5'- triphosphate; C573392:2'-methylseleno-2'-deoxyguanosine-5'-triphosphate; D003838:Deoxyadenine Nucleotides; D003848:Deoxyguanine Nucleotides; D016566:Organoselenium Compounds; D014764:Viral Proteins; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases; D012643:Selenium