title: Application of autologous cultivated corneal epithelium for corneal limbal stem cell insufficiency--short-term results.
abstract: OBJECTIVE
  To present results of ocular surface reconstruction with application of cultured corneal epithelium in limbal stem cell insufficiency.
  
  
  METHODS
  25 patients suffered from limbal insufficiency in one eye after a chemical or thermal burn. Their healthy eyes were the source of limbal epithelium for corneal epithelial culture. Limbal cells from a 2 mm2 biopsy were seeded on amniotic membrane. Cultures were carried out in standard conditions in supplemented DMEM in presence of 3T3 fibroblasts. After superficial keratectomy the amniotic carriers with epithelial cells were transplanted on denuded corneas. The presence of corneal vascularization, epithelial regularity and visual acuity were evaluated.
  
  
  RESULTS
  Postoperative follow-up ranged from 3 up to 9 months, average 4.9 +/- 1.1 months. 3 months after the surgery 72.0% of the eyes showed stable epithelium with slight corneal haze caused by the amnion. In 60.0% of the eyes there was no recurrent conjunctival neovascularization. 28.0% of the eyes remained cloudy due to stromal revascularization. In 2 eyes total conjunctival pannus developed again. Visual acuity ranged from counting fingers to 0.6.
  
  
  CONCLUSIONS
  Transplantation of cultured epithelium is a promising method in treatment of limbal stem cell insufficiency giving satisfactory restoration of corneal epithelium.
authors.affiliation: Department of Ophthalmology, District Railway Hospital, Katowice, Poland. dardobmd@wp.pl
authors.forename: Dariusz
authors.initials: D
authors.lastname: Dobrowolski
authors.forename: Edward
authors.initials: E
authors.lastname: Wylegala
authors.forename: Bogusława
authors.initials: B
authors.lastname: Orzechowska-Wylegala
authors.forename: Bogumił
authors.initials: B
authors.lastname: Wowra
authors.forename: Ewa
authors.initials: E
authors.lastname: Wróblewska-Czajka
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002057:Burns, Chemical / Q000601:surgery; D018929:Cell Culture Techniques / Q000379:methods; D017690:Cell Transplantation / Q000379:methods*; D003315:Cornea / Q000601:surgery; D003316:Corneal Diseases / Q000601:surgery; D004847:Epithelial Cells / Q000166:cytology* / Q000637:transplantation*; D019573:Epithelium, Corneal / Q000166:cytology* / Q000637:transplantation*; D005126:Eye Burns / Q000601:surgery*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D008875:Middle Aged; D011044:Poland; D033581:Stem Cell Transplantation; D014182:Transplantation, Autologous; D016896:Treatment Outcome; D055815:Young Adult