title: Bacillus thuringiensis protein transfer between rootstock and scion of grafted poplar.
abstract: Bacillus thuringiensis (Bt) Cry1Ac protein is a toxin against different leaf-eating lepidopteran insects that attack poplar trees. In the present study, the mode of migration of the Bt-Cry1Ac protein within poplar grafts was investigated. Grafting was done using Pb29 (transgenic poplar 741 with cry1Ac genes), CC71 (transgenic poplar 741 with cry3A genes), non-transgenic poplar 741 and non-transgenic Populus tomentosa, either as scion or as rootstock. In order to detect migration of Bt-Cry1Ac protein from one portion of the graft union to different tissues in the grafted plant, ELISA analysis was employed to assess the content of Bt-Cry1Ac protein in the phloem, xylem, pith and leaves of the grafted poplar. To further verify migration of Bt-Cry1Ac protein, Clostera anachoreta larvae, which are susceptible to Bt-Cry1Ac protein, were fed leaves from the control graft (i.e., graft portion that originally did not contain Bt-Cry1Ac protein). The results showed that Bt-Cry1Ac protein was transported between rootstock and scion mainly through the phloem. Migration of Bt-Cry1Ac protein in the grafted union was also evidenced in that the leaves of the control graft did have a lethal effect on C. anachoreta larvae in laboratory feeding experiments.
authors.affiliation: Institute of Forest Biotechnology, Forestry College, Agricultural University of Hebei, Baoding, China College of Horticulture, Hebei North University, Zhangjiakou, China Department of Forestry & Wood Technology, Federal University of Technology, Akure, Nigeria Institute of Forest Genetics, Johann Heinrich von Thuenen Institute Federal Research Institute for Rural Areas, Forestry and Fisheries, Waldsieversdorf, Germany.
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.affiliation: Institute of Forest Biotechnology, Forestry College, Agricultural University of Hebei, Baoding, China College of Horticulture, Hebei North University, Zhangjiakou, China Department of Forestry & Wood Technology, Federal University of Technology, Akure, Nigeria Institute of Forest Genetics, Johann Heinrich von Thuenen Institute Federal Research Institute for Rural Areas, Forestry and Fisheries, Waldsieversdorf, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Yang
authors.affiliation: Institute of Forest Biotechnology, Forestry College, Agricultural University of Hebei, Baoding, China College of Horticulture, Hebei North University, Zhangjiakou, China Department of Forestry & Wood Technology, Federal University of Technology, Akure, Nigeria Institute of Forest Genetics, Johann Heinrich von Thuenen Institute Federal Research Institute for Rural Areas, Forestry and Fisheries, Waldsieversdorf, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Akinnagbe
authors.affiliation: Institute of Forest Biotechnology, Forestry College, Agricultural University of Hebei, Baoding, China College of Horticulture, Hebei North University, Zhangjiakou, China Department of Forestry & Wood Technology, Federal University of Technology, Akure, Nigeria Institute of Forest Genetics, Johann Heinrich von Thuenen Institute Federal Research Institute for Rural Areas, Forestry and Fisheries, Waldsieversdorf, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Liang
authors.affiliation: Institute of Forest Biotechnology, Forestry College, Agricultural University of Hebei, Baoding, China College of Horticulture, Hebei North University, Zhangjiakou, China Department of Forestry & Wood Technology, Federal University of Technology, Akure, Nigeria Institute of Forest Genetics, Johann Heinrich von Thuenen Institute Federal Research Institute for Rural Areas, Forestry and Fisheries, Waldsieversdorf, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.affiliation: Institute of Forest Biotechnology, Forestry College, Agricultural University of Hebei, Baoding, China College of Horticulture, Hebei North University, Zhangjiakou, China Department of Forestry & Wood Technology, Federal University of Technology, Akure, Nigeria Institute of Forest Genetics, Johann Heinrich von Thuenen Institute Federal Research Institute for Rural Areas, Forestry and Fisheries, Waldsieversdorf, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Ewald
mesh_terms: D000818:Animals; D001413:Bacillus thuringiensis / Q000378:metabolism*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000378:metabolism*; D004731:Endotoxins / Q000378:metabolism*; D006460:Hemolysin Proteins / Q000378:metabolism*; D007814:Larva / Q000254:growth & development; D007915:Lepidoptera / Q000502:physiology; D018515:Plant Leaves / Q000378:metabolism; D030821:Plants, Genetically Modified; D032107:Populus / Q000378:metabolism*; D021381:Protein Transport
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis