title: Barramundi (Lates calcarifer) desaturase with Δ6/Δ8 dual activities.
abstract: Barramundi is a commercially farmed fish in Australia. To examine the potential for barramundi to metabolise dietary α-linolenic acid (ALA, 18:3 n-3), the existence of barramundi desaturase enzymes was examined. A putative fatty acid Δ6 desaturase was cloned from barramundi liver and expressed in yeast. Functional expression revealed Δ6 desaturase activity with both the 18 carbon (C(18)) and C(24) n-3 fatty acids, ALA and 24:5 n-3 as well as the C(18) n-6 fatty, linoleic acid (LA, 18:2 n-6). Metabolism of ALA was favoured over LA. The enzyme also had Δ8 desaturase activity which raises the potential for synthesis in barramundi of omega-3 (n-3) long chain polyunsaturated fatty acids from ALA via a pathway that bypasses the initial Δ6 desaturase step. Our findings not only provide molecular evidence for the fatty acid desaturation pathway in the barramundi but also highlight the importance of taking extracellular fatty acid levels into account when assessing enzyme activity expressed in Saccharomyces cerevisiae.
authors.affiliation: FOODplus Research Centre, School of Agriculture, Food and Wine, Waite Campus, University of Adelaide, Adelaide, SA, Australia.
authors.forename: Wei-Chun
authors.initials: WC
authors.lastname: Tu
authors.forename: Rebecca J
authors.initials: RJ
authors.lastname: Cook-Johnson
authors.forename: Michael J
authors.initials: MJ
authors.lastname: James
authors.forename: Beverly S
authors.initials: BS
authors.lastname: Mühlhäusler
authors.forename: David A J
authors.initials: DA
authors.lastname: Stone
authors.forename: Robert A
authors.initials: RA
authors.lastname: Gibson
mesh_terms: D000818:Animals; D001315:Australia; D003001:Cloning, Molecular; D044943:Fatty Acid Desaturases / Q000235:genetics / Q000378:metabolism*; D005399:Fishes; D015870:Gene Expression; D008099:Liver / Q000201:enzymology*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D017962:alpha-Linolenic Acid / Q000378:metabolism*
chemical_list: D017962:alpha-Linolenic Acid; D044943:Fatty Acid Desaturases