title: Nonomuraea jabiensis sp. nov., isolated from arid soil.
abstract: A novel actinomycete, strain A4036(T), was isolated from a soil sample collected from the Jabi district in Abuja, Nigeria. The taxonomic position of strain A4036(T) was established using a combination of genotypic and phenotypic analyses. The organism formed extensively branched substrate and aerial hyphae that generated spiral chains of spores with warty surfaces. The cell wall contained meso-diaminopimelic acid and the cell-wall sugars were glucose, madurose, mannose and ribose. The predominant menaquinone was MK-9(H(4)). The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylmethylethanolamine, phosphatidylinositol mannoside, hydroxy-phosphatidylethanolamine, hydroxy-phosphatidylmethylethanolamine, two unidentified phospholipids and four unknown glucosamine-containing phospholipids. The major cellular fatty acids were iso-C(16 : 0) 2-OH, iso-C(16 : 0) and 10-methyl C(17 : 0). On the basis of 16S rRNA gene sequence similarity studies, strain A4036(T) grouped in the genus Nonomuraea, being most closely related to Nonomuraea angiospora IFO 13155(T) (99.05 %), Nonomuraea candida HMC10(T) (98.78 %), Nonomuraea kuesteri GW 14-1925(T) (98.49 %), Nonomuraea endophytica YIM 65601(T) (98.42 %), Nonomuraea maheshkhaliensis 16-5-14(T) (98.40 %), Nonomuraea turkmeniaca DSM 43926(T) (98.38 %), Nonomuraea helvata IFO 14681(T) (98.29 %), Nonomuraea rubra DSM 43768(T) (98.10 %) and Nonomuraea salmonea DSM 43678(T) (98.06 %). Levels of 16S rRNA gene sequence similarity to the type strains of other species of the genus Nonomuraea were <98 %. Despite the high 16S rRNA gene sequence similarities, DNA-DNA relatedness values and phenotypic data demonstrated that strain A4036(T) was clearly distinguished from all closely related species of the genus Nonomuraea. Thus, this isolate is considered to represent a novel species of the genus Nonomuraea, for which the name Nonomuraea jabiensis sp. nov. is proposed. The type strain is A4036(T) (= DSM 45507(T) = KCTC 19870(T)).
authors.affiliation: Department of Biology, Faculty of Art and Science, Ondokuz Mayis University, 55139 Kurupelit-Samsun, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Camas
authors.affiliation: Department of Biology, Faculty of Art and Science, Ondokuz Mayis University, 55139 Kurupelit-Samsun, Turkey.
authors.forename: Anil
authors.initials: A
authors.lastname: Sazak
authors.affiliation: German Collection of Microorganisms and Cell Cultures GmbH, 38124 Braunschweig, Germany.
authors.forename: Cathrin
authors.initials: C
authors.lastname: Spröer
authors.affiliation: German Collection of Microorganisms and Cell Cultures GmbH, 38124 Braunschweig, Germany.
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Klenk
authors.affiliation: Science Teaching Programme, Gazi Faculty of Education, Gazi University, 06500 Ankara, Turkey.
authors.forename: Demet
authors.initials: D
authors.lastname: Cetin
authors.affiliation: Department of Biology, Faculty of Science, Anadolu University, 26470 Eskisehir, Turkey.
authors.forename: Kiymet
authors.initials: K
authors.lastname: Guven
authors.affiliation: Department of Biology, Faculty of Art and Science, Ondokuz Mayis University, 55139 Kurupelit-Samsun, Turkey.
authors.forename: Nevzat
authors.initials: N
authors.lastname: Sahin
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009549:Nigeria; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9