title: Caenorhabditis elegans maintains highly compartmentalized cellular distribution of metals and steep concentration gradients of manganese.
abstract: Bioinorganic chemistry is critical to cellular function. Homeostasis of manganese (Mn), for example, is essential for life. A lack of methods for direct in situ visualization of Mn and other biological metals within intact multicellular eukaryotes limits our understanding of management of these metals. We provide the first quantitative subcellular visualization of endogenous Mn concentrations (spanning two orders of magnitude) associated with individual cells of the nematode, Caenorhabditis elegans.
authors.affiliation: Mental Health Research Institute, University of Melbourne, Parkville, Australia.
authors.forename: Gawain
authors.initials: G
authors.lastname: McColl
authors.forename: Simon A
authors.initials: SA
authors.lastname: James
authors.forename: Sheridan
authors.initials: S
authors.lastname: Mayo
authors.forename: Daryl L
authors.initials: DL
authors.lastname: Howard
authors.forename: Christopher G
authors.initials: CG
authors.lastname: Ryan
authors.forename: Robin
authors.initials: R
authors.lastname: Kirkham
authors.forename: Gareth F
authors.initials: GF
authors.lastname: Moorhead
authors.forename: David
authors.initials: D
authors.lastname: Paterson
authors.forename: Martin D
authors.initials: MD
authors.lastname: de Jonge
authors.forename: Ashley I
authors.initials: AI
authors.lastname: Bush
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000187:drug effects / Q000378:metabolism*; D027682:Cation Transport Proteins / Q000737:chemistry; D019295:Computational Biology / Q000379:methods; D005612:Freeze Drying; D021621:Imaging, Three-Dimensional; D007413:Intestinal Mucosa / Q000378:metabolism; D008345:Manganese / Q000737:chemistry*; D008670:Metals / Q000737:chemistry*; D010100:Oxygen / Q000737:chemistry; D014057:Tomography, X-Ray Computed / Q000379:methods
chemical_list: D027682:Cation Transport Proteins; D008670:Metals; D008345:Manganese; D010100:Oxygen