title: A simple boiling-based DNA  extraction for RAPD profiling of landfarm soil to provide representative  metagenomic content.
abstract: Landfarm  soils are employed in industrial and petrochemical residue bioremediation. This  process induces selective pressure directed towards microorganisms capable of  degrading toxic compounds. Detailed description of taxa in these environments  is difficult due to a lack of knowledge of culture conditions required for  unknown microorganisms. A metagenomic approach permits identification of  organisms without the need for culture. However, a DNA extraction step is first  required, which can bias taxonomic representativeness and interfere with  cloning steps by extracting interference substances. We developed a simplified  DNA extraction procedure coupled with metagenomic DNA amplification in an  effort to overcome these limitations. The amplified sequences were used to  generate a metagenomic data set and the taxonomic and functional  representativeness were evaluated in comparison with a data set built with DNA  extracted by conventional methods. The simplified and optimized method of RAPD  to access metagenomic information provides better representativeness of the  taxonomical and metabolic aspects of the environmental samples.
authors.affiliation: Departamento de Microbiologia, Universidade de São Paulo, São Paulo, Brasil.
authors.forename: J H
authors.initials: JH
authors.lastname: Amorim
authors.forename: R O
authors.initials: RO
authors.lastname: Vidal
authors.forename: G V
authors.initials: GV
authors.lastname: Lacerda-Junior
authors.forename: J C T
authors.initials: JC
authors.lastname: Dias
authors.forename: M
authors.initials: M
authors.lastname: Brendel
authors.forename: R P
authors.initials: RP
authors.lastname: Rezende
authors.forename: J C M
authors.initials: JC
authors.lastname: Cascardo
mesh_terms: D000383:Agriculture / Q000379:methods; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D054892:Metagenome / Q000235:genetics; D056186:Metagenomics / Q000379:methods*; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D012987:Soil / Q000032:analysis; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D012987:Soil