title: Freeze-dried human serum albumin improves the adherence and proliferation of mesenchymal stem cells on mineralized human bone allografts.
abstract: Mineralized scaffolds are widely used as bone grafts with the assumption that bone marrow derived cells colonize and remodel them. This process is slow and often unreliable so we aimed to improve the biocompatibility of bone grafts by pre-seeding them with human mesenchymal stem cells from either bone marrow or dental pulp. Under standard cell culture conditions very low number of seeded cells remained on the surface of freeze-dried human or bovine bone graft or hydroxyapatite. Coating the scaffolds with fibronectin or collagen improved seeding efficiency but the cells failed to grow on the surface until the 18th day. In contrast, human albumin was a very potent facilitator of both seeding and proliferation on allografts which was further improved by culturing in a rotating bioreactor. Electron microscopy revealed that cells do not form a monolayer but span the pores, emphasizing the importance of pore size and microstructure. Albumin coated bone chips were able to unite a rat femoral segmental defect, while uncoated ones did not. Micro-hardness measurements confirmed that albumin coating does not influence the physical characteristics of the scaffold, so it is possible to introduce albumin coating into the manufacturing process of lyophilized bone allografts.
authors.affiliation: Tissue Engineering Laboratory, Department of Human Physiology and Clinical Experimental Research, Semmelweis University, 1094 Budapest Tu"zolt utcaó 37-47, Budapest 1094, Hungary. miklos.weszl@gmail.com
authors.forename: Miklós
authors.initials: M
authors.lastname: Weszl
authors.forename: Gábor
authors.initials: G
authors.lastname: Skaliczki
authors.forename: Attila
authors.initials: A
authors.lastname: Cselenyák
authors.forename: Levente
authors.initials: L
authors.lastname: Kiss
authors.forename: Tibor
authors.initials: T
authors.lastname: Major
authors.forename: Károly
authors.initials: K
authors.lastname: Schandl
authors.forename: Eszter
authors.initials: E
authors.lastname: Bognár
authors.forename: Guido
authors.initials: G
authors.lastname: Stadler
authors.forename: Anja
authors.initials: A
authors.lastname: Peterbauer
authors.forename: Lajos
authors.initials: L
authors.lastname: Csönge
authors.forename: Zsombor
authors.initials: Z
authors.lastname: Lacza
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D019149:Bioreactors; D001854:Bone Marrow Cells / Q000502:physiology; D016025:Bone Transplantation; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D024042:Collagen Type I / Q000502:physiology*; D003782:Dental Pulp / Q000166:cytology; D005353:Fibronectins / Q000502:physiology*; D005612:Freeze Drying; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012709:Serum Albumin / Q000502:physiology*; D013552:Swine; D054457:Tissue Scaffolds; D014184:Transplantation, Homologous; D055815:Young Adult
chemical_list: D024042:Collagen Type I; D005353:Fibronectins; D012709:Serum Albumin