title: Direct comparison of different stem cell types and subpopulations reveals superior paracrine potency and myocardial repair efficacy with cardiosphere-derived cells.
abstract: OBJECTIVE
  The goal of this study was to conduct a direct head-to-head comparison of different stem cell types in vitro for various assays of potency and in vivo for functional myocardial repair in the same mouse model of myocardial infarction.
  
  
  BACKGROUND
  Adult stem cells of diverse origins (e.g., bone marrow, fat, heart) and antigenic identity have been studied for repair of the damaged heart, but the relative utility of the various cell types remains unclear.
  
  
  METHODS
  Human cardiosphere-derived cells (CDCs), bone marrow-derived mesenchymal stem cells, adipose tissue-derived mesenchymal stem cells, and bone marrow mononuclear cells were compared.
  
  
  RESULTS
  CDCs revealed a distinctive phenotype with uniform expression of CD105, partial expression of c-kit and CD90, and negligible expression of hematopoietic markers. In vitro, CDCs showed the greatest myogenic differentiation potency, highest angiogenic potential, and relatively high production of various angiogenic and antiapoptotic-secreted factors. In vivo, injection of CDCs into the infarcted mouse hearts resulted in superior improvement of cardiac function, the highest cell engraftment and myogenic differentiation rates, and the least-abnormal heart morphology 3 weeks after treatment. CDC-treated hearts also exhibited the lowest number of apoptotic cells. The c-kit(+) subpopulation purified from CDCs produced lower levels of paracrine factors and inferior functional benefit when compared with unsorted CDCs. To validate the comparison of cells from various human donors, selected results were confirmed in cells of different types derived from individual rats.
  
  
  CONCLUSIONS
  CDCs exhibited a balanced profile of paracrine factor production and, among various comparator cell types/subpopulations, provided the greatest functional benefit in experimental myocardial infarction.
authors.affiliation: Cedars-Sinai Heart Institute, Los Angeles, California, USA.
authors.forename: Tao-Sheng
authors.initials: TS
authors.lastname: Li
authors.forename: Ke
authors.initials: K
authors.lastname: Cheng
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Malliaras
authors.forename: Rachel Ruckdeschel
authors.initials: RR
authors.lastname: Smith
authors.forename: Yiqiang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Baiming
authors.initials: B
authors.lastname: Sun
authors.forename: Noriko
authors.initials: N
authors.lastname: Matsushita
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Blusztajn
authors.forename: John
authors.initials: J
authors.lastname: Terrovitis
authors.forename: Hideo
authors.initials: H
authors.lastname: Kusuoka
authors.forename: Linda
authors.initials: L
authors.lastname: Marbán
authors.forename: Eduardo
authors.initials: E
authors.lastname: Marbán
mesh_terms: D000418:Albumins / Q000494:pharmacology; D000818:Animals; D017209:Apoptosis; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006801:Humans; D020287:In Situ Nick-End Labeling; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D016513:Mice, SCID; D009203:Myocardial Infarction / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D009206:Myocardium / Q000166:cytology*; D032383:Myocytes, Cardiac / Q000166:cytology / Q000637:transplantation*; D019899:Paracrine Communication / Q000502:physiology*; D011091:Polyesters / Q000494:pharmacology; D051381:Rats; D011921:Rats, Inbred WKY; D033581:Stem Cell Transplantation; D014945:Wound Healing
chemical_list: D000418:Albumins; C522044:PB-127 compound; D011091:Polyesters