title: Multi-wavelength anomalous diffraction using medium-angle X-ray solution scattering (MADMAX).
abstract: Proteins are dynamic molecules whose function in virtually all biological processes requires conformational motion. Direct experimental probes of protein structure in solution are needed to characterize these motions. Anomalous scattering from proteins in solution has the potential to act as a precise molecular ruler to determine the positions of specific chemical groups or atoms within proteins under conditions in which structural changes can take place free from the constraints of crystal contacts. In solution, anomalous diffraction has two components: a set of cross-terms that depend on the relative location of the anomalous centers and the rest of the protein, and a set of pure anomalous terms that depend on the distances between the anomalous centers. The cross-terms are demonstrated here to be observable and to provide direct information about the distance between the anomalous center and the center of mass of the protein. The second set of terms appears immeasurably small in the context of current experimental capabilities. Here, we outline the theory underlying anomalous scattering from proteins in solution, predict the anomalous differences expected on the basis of atomic coordinate sets, and demonstrate the measurement of anomalous differences at the iron edge for solutions of myoglobin and hemoglobin.
authors.affiliation: Department of Electrical and Computer Engineering, Northeastern University, Boston, Massachusetts, USA. makowski@ece.neu.edu
authors.forename: L
authors.initials: L
authors.lastname: Makowski
authors.forename: J
authors.initials: J
authors.lastname: Bardhan
authors.forename: D
authors.initials: D
authors.lastname: Gore
authors.forename: D J
authors.initials: DJ
authors.lastname: Rodi
authors.forename: R F
authors.initials: RF
authors.lastname: Fischetti
mesh_terms: D000818:Animals; D006441:Hemoglobin A / Q000737:chemistry*; D006801:Humans; D009211:Myoglobin / Q000737:chemistry*; D012996:Solutions; D014961:X-Ray Diffraction / Q000379:methods*
chemical_list: D009211:Myoglobin; D012996:Solutions; D006441:Hemoglobin A