title: [Cases of Toxoplasma gondii infection in foetus's and toxoplasmic encephalitis in immunosupressed patients confirmed by PCR method in NIPH - NIH, 2009-2010].
abstract: The results of molecular tests for Toxoplasma gondii infection carried out in NIPH-NIH were presented in the light of current data on diagnostics and epidemiology of toxoplasmosis. Between January 2009 and December 2010 four cases of active toxoplasmosis were confirmed using PCR targeting the 529-bp repeat element of Toxoplasma gondii. Intrauterine infection was found in 3 out of 180 (1.7%) examined women. Toxoplasmic encephalitis was confirmed in one patient who was a liver transplant recipient. T. gondii was not detected in cerebrospinal fluid of 19 out of 20 examined patients with encephalitis. The cases of intrauterine toxoplasmosis diagnosed in 2009-2010 make 57.1% of all cases diagnosed in the last decade. In 2001-2010 toxoplasmic encephalitis was detected in two patients out of 45 examined. The choice of tests and interpretation of the results are important elements of T. gondii infection diagnosis and require cooperation between clinicians and laboratory diagnosticians. Establishing reference center for toxoplasmosis in Poland would contribute towards the improvement of standards of diagnostics and treatment of cases of congenital infections and the infection in immunosuppresed patients.
authors.affiliation: Zakład Parazytologii Lekarskiej Narodowego Instytutu Zdrowia Publicznego - Państwowego Zakładu Higieny w Warszawie.
authors.forename: Wioletta
authors.initials: W
authors.lastname: Rozej-Bielicka
authors.forename: Maria
authors.initials: M
authors.lastname: Waloch
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Gołab
mesh_terms: D000328:Adult; D015897:Comorbidity; D005260:Female; D005315:Fetal Diseases / Q000175:diagnosis* / Q000453:epidemiology* / Q000469:parasitology; D006801:Humans; D016867:Immunocompromised Host; D008297:Male; D008431:Maternal-Fetal Exchange; D008875:Middle Aged; D011044:Poland / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000379:methods; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000175:diagnosis* / Q000453:epidemiology* / Q000469:parasitology; D012307:Risk Factors; D014122:Toxoplasma / Q000235:genetics / Q000302:isolation & purification*; D014123:Toxoplasmosis / Q000175:diagnosis* / Q000453:epidemiology*; D016781:Toxoplasmosis, Cerebral / Q000175:diagnosis / Q000453:epidemiology