title: Effect of the heat shock protein HSP27 on androgen receptor expression and function in prostate cancer cells.
abstract: OBJECTIVE
  Heat shock proteins (HSP) are involved in processes of folding, activation, trafficking and transcriptional activity of most steroid receptors including the androgen receptor (AR). Accumulating evidence links rising heat shock protein 27 (HSP27) levels with the development of castration-resistant prostate cancer. In order to study the functional relationship between HSP27 and the AR, we modulated the expression of the small heat shock protein HSP27 in human prostate cancer (PC) cell lines.
  
  
  METHODS
  HSP27 protein concentrations in LNCaP and PC-3 cells were modulated by over-expression or silencing of HSP27. The effects of HSP27 on AR protein and mRNA levels were monitored by Western blotting and quantitative RT-PCR.
  
  
  RESULTS
  Treatment for the AR-positive LNCaP with HSP27-specific siRNA resulted in a down-regulation of AR levels. This down-regulation of protein was paralleled by a decrease in AR mRNA. Most interestingly, over-expression of HSP27 in PC-3 cells led to a significant increase in AR mRNA although the cells were unable to produce functional AR protein.
  
  
  CONCLUSIONS
  The observation that HSP27 is involved in the regulation of AR mRNA by a yet unknown mechanism highlights the complexity of HSP27-AR signaling network.
authors.affiliation: Department of Urology, Ernst Moritz Arndt University of Greifswald, Ferdinand-Sauerbruch-Straße, 17475, Greifswald, Germany. matthias.stope@uni-greifswald.de
authors.forename: Matthias B
authors.initials: MB
authors.lastname: Stope
authors.forename: Tina
authors.initials: T
authors.lastname: Schubert
authors.forename: Doreen
authors.initials: D
authors.lastname: Staar
authors.forename: Cindy
authors.initials: C
authors.lastname: Rönnau
authors.forename: Andreas
authors.initials: A
authors.lastname: Streitbörger
authors.forename: Nils
authors.initials: N
authors.lastname: Kroeger
authors.forename: Constanze
authors.initials: C
authors.lastname: Kubisch
authors.forename: Uwe
authors.initials: U
authors.lastname: Zimmermann
authors.forename: Reinhard
authors.initials: R
authors.lastname: Walther
authors.forename: Martin
authors.initials: M
authors.lastname: Burchardt
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D055551:HSP27 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology*; D012333:RNA, Messenger / Q000378:metabolism; D011944:Receptors, Androgen / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology; D014162:Transfection
chemical_list: D055551:HSP27 Heat-Shock Proteins; D012333:RNA, Messenger; D011944:Receptors, Androgen