title: A perivascular system releasing sirolimus prevented intimal hyperplasia in a rabbit model in a medium-term study.
abstract: The main complication of aortocoronary reconstruction with vein grafts is restenosis in the course of time. The aim was to assess the effect of a periadventitial polyester mesh releasing sirolimus on intimal hyperplasia of autologous grafts. We implanted v. jugularis ext. into a. carotis communis in rabbits. The vein graft was either intact, or was wrapped with a pure polyester mesh, or with a sirolimus-releasing mesh. Three and six weeks after surgery, the veins were subjected to standard histological staining and the thicknesses of the tunica intima, the media and the intima-media complex were measured. Wrapping the vein with a mesh releasing sirolimus or with a pure mesh decreased the thickness of the intima in comparison with a vein graft by 73 ± 11% or 73 ± 8% after 3 weeks, and by 73 ± 9% or 59 ± 12% after 6 weeks, respectively. Sirolimus-releasing meshes reduced the thickness of the media by 65 ± 9% and 20 ± 12% after 3 and 6 weeks. The thickness of the intima-media complex in grafts with sirolimus-releasing meshes decreased by 60 ± 6% and 30 ± 13% in comparison with pure PES meshes, after 3 and 6 weeks, respectively. A periadventitial polyester mesh releasing sirolimus has the potential to become an effective device in preventing vein graft restenosis.
authors.affiliation: Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21 Prague 4, Czech Republic. ivo.skalsky@ikem.cz
authors.forename: Ivo
authors.initials: I
authors.lastname: Skalský
authors.forename: Ondrej
authors.initials: O
authors.lastname: Szárszoi
authors.forename: Elena
authors.initials: E
authors.lastname: Filová
authors.forename: Martin
authors.initials: M
authors.lastname: Pařízek
authors.forename: Andriy
authors.initials: A
authors.lastname: Lytvynets
authors.forename: Jana
authors.initials: J
authors.lastname: Malušková
authors.forename: Alena
authors.initials: A
authors.lastname: Lodererová
authors.forename: Eduard
authors.initials: E
authors.lastname: Brynda
authors.forename: Věra
authors.initials: V
authors.lastname: Lisá
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Burdíková
authors.forename: Martin
authors.initials: M
authors.lastname: Capek
authors.forename: Jan
authors.initials: J
authors.lastname: Pirk
authors.forename: Lucie
authors.initials: L
authors.lastname: Bačáková
mesh_terms: D000818:Animals; D002452:Cell Count; D049109:Cell Proliferation; D002682:Chinchilla; D004343:Drug Implants; D006083:Graft Occlusion, Vascular / Q000473:pathology / Q000517:prevention & control*; D006965:Hyperplasia / Q000473:pathology; D007150:Immunohistochemistry; D007166:Immunosuppressive Agents / Q000008:administration & dosage* / Q000627:therapeutic use*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000187:drug effects; D016612:Paraffin Embedding; D011091:Polyesters; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D011817:Rabbits; D020123:Sirolimus / Q000008:administration & dosage* / Q000627:therapeutic use*; D013526:Surgical Mesh; D016707:Tissue Fixation; D017539:Tunica Intima / Q000473:pathology*; D017540:Tunica Media / Q000473:pathology; D014680:Veins / Q000637:transplantation
chemical_list: D004343:Drug Implants; D007166:Immunosuppressive Agents; D011091:Polyesters; D018809:Proliferating Cell Nuclear Antigen; D020123:Sirolimus