title: Molecular cloning, characterization and expression analysis of B cell translocation gene 1 in grass carp Ctenopharyngodon idella.
abstract: An expressed sequence tag (EST) of B cell translocation gene (BTG) 1 (gcbtg1) was obtained from a grass carp Ctenopharyngodon idellus intestinal complementary (c)DNA library and the full-length cDNA sequence was acquired by rapid amplification of cDNA ends (RACE) technology. The predicted Gcbtg1 protein contains the box A and box B motifs which characterized the BTG and transducer of ERBB2 (TOB) family. Multiple alignment analysis reveals that Gcbtg1 shares an overall identity of 65-94% with Gcbtg1s of other vertebrates. Real-time quantitative PCR analysis reveals that the highest expression level of gcbtg1 was detected in liver and the lowest in muscle. Western blotting analysis indicates that the immunological cross-reactivity occurs between C. idella and human Homo sapiens BTG1 protein. A 1008 bp 5'-flanking region sequence was cloned and analysed.
authors.affiliation: School of Life Sciences, Sichuan University, Chengdu 610064, PR China.
authors.forename: Y J
authors.initials: YJ
authors.lastname: Fu
authors.forename: F G
authors.initials: FG
authors.lastname: Huang
authors.forename: T
authors.initials: T
authors.lastname: Yuan
authors.forename: J R
authors.initials: JR
authors.lastname: Gu
authors.forename: G Q
authors.initials: GQ
authors.lastname: Luo
authors.forename: H
authors.initials: H
authors.lastname: Xu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D020224:Expressed Sequence Tags; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000737:chemistry; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D017422:Sequence Analysis, DNA
chemical_list: D029941:Fish Proteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger; C074086:BTG1 protein, human