title: DeSUMOylating isopeptidase: a second class of SUMO protease.
abstract: The modification of proteins by small ubiquitin-like modifier (SUMO) is crucial for the regulation of diverse cellular processes. Protein SUMOylation is reversed by isopeptidases, collectively known as deSUMOylases. Only one family of SUMO-specific proteases has been described so far: the sentrin-specific proteases (SENP). Here, we identify and characterize a new deSUMOylase, which we have named DeSI-1 (DeSumoylating Isopeptidase 1). We describe BZEL—a new transcriptional repressor—as substrate of DeSI-1. DeSI-1 catalyses the deSUMOylation, but not the deubiquitination, of BZEL. Furthermore, the SENP substrates PML and ΔNp63 are not deSUMOylated by DeSI-1, suggesting that SENP and DeSI enzymes recognize different sets of substrates. Together, these data identify a second class of SUMO proteases.
authors.affiliation: Department of Life Science, Ewha Womans University, Seoul 120-750, Korea.
authors.forename: Eun Ju
authors.initials: EJ
authors.lastname: Shin
authors.forename: Hyun Mi
authors.initials: HM
authors.lastname: Shin
authors.forename: Eori
authors.initials: E
authors.lastname: Nam
authors.forename: Won Seog
authors.initials: WS
authors.lastname: Kim
authors.forename: Ji-Hoon
authors.initials: JH
authors.lastname: Kim
authors.forename: Byung-Ha
authors.initials: BH
authors.lastname: Oh
authors.forename: Yungdae
authors.initials: Y
authors.lastname: Yun
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019759:Carbon-Nitrogen Lyases / Q000737:chemistry / Q000378:metabolism*; D002460:Cell Line; D010450:Endopeptidases / Q000378:metabolism; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000378:metabolism; D012097:Repressor Proteins / Q000378:metabolism; D025841:Small Ubiquitin-Related Modifier Proteins / Q000378:metabolism*; D013379:Substrate Specificity; D058207:Sumoylation; D014158:Transcription, Genetic
chemical_list: D011994:Recombinant Proteins; D012097:Repressor Proteins; D025841:Small Ubiquitin-Related Modifier Proteins; D010450:Endopeptidases; D019759:Carbon-Nitrogen Lyases; C574164:deSumoylating Isopeptidase 1, mouse; C034300:isopeptidase