title: Viability, maturation and embryo development in vitro of immature porcine and ovine oocytes vitrified in different devices.
abstract: This study was designed to evaluate the effects of vitrification on immature porcine and ovine oocytes, collected at a slaughterhouse, by performing vitrification in devices with different volumes. Viability was evaluated both before and after vitrification and maturation. Immediately after warming, the percentage of viable pig oocytes was 81% regardless the type of device, while in the control (after oocyte selection) was 95%. The viability of matured pig oocytes after warming, vitrified in beveled edge open straws (BES) was 6%, in small-open-pulled-straw (SOPS) was 17% and in cryotop was 4%, while the viability of the control group was 86%. The viability and maturation results were similar with all devices. Embryo development (ED) was observed in fresh porcine oocytes with 15% 2-8 cell embryos, 7% morulae and 3% blastocysts, and non-embryo cleavage was observed in warmed oocytes. The viability of sheep oocytes immediately after warming averaged 90% in all devices, while that of the control (after oocyte selection) averaged 95%. The viability of warmed oocytes after maturation was: BES 21%, SOPS 30%, cryotop 21% and control group 86%; while maturation values were 11, 21, 34 and 70%, respectively. After vitrification, the highest ED was achieved with ovine oocytes vitrified in SOPS, with 17% morulae development and it was the only device in which blastocysts developed. A direct relationship was observed between viability and actin filament integrity in both species.
authors.affiliation: Departamento de Producción Agrícola y Animal, División de Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana-Xochimilco, Calzada del Hueso 1100, 04960 DF, Mexico. freyes@correo.xoc.uam.mx
authors.forename: Filiberto
authors.initials: F
authors.lastname: Fernández-Reyez
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Ducolomb
authors.forename: Salvador
authors.initials: S
authors.lastname: Romo
authors.forename: Eduardo
authors.initials: E
authors.lastname: Casas
authors.forename: Zayil
authors.initials: Z
authors.lastname: Salazar
authors.forename: Miguel
authors.initials: M
authors.lastname: Betancourt
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000494:pharmacology; D047108:Embryonic Development / Q000187:drug effects / Q000502:physiology*; D005260:Female; D005307:Fertilization in Vitro; D008297:Male; D009028:Morula / Q000187:drug effects; D009865:Oocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D009866:Oogenesis / Q000187:drug effects / Q000502:physiology; D012661:Semen / Q000502:physiology; D012756:Sheep; D034561:Sheep, Domestic; D013552:Swine; D058989:Vitrification
chemical_list: D003451:Cryoprotective Agents