title: Microdissection of Drosophila polytene chromosomes for DOP-PCR.
abstract: This protocol describes a method for the microdissection of Drosophila polytene chromosomes. The microdissected DNA can be amplified by degenerate oligonucleotide primed-polymerase chain reaction (DOP-PCR) and then used to prepare probes for fluorescent in situ hybridization (FISH).
authors.forename: Abby F
authors.initials: AF
authors.lastname: Dernburg
mesh_terms: D000818:Animals; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D017931:DNA Primers / Q000235:genetics; D004330:Drosophila / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D042282:Microdissection / Q000379:methods*; D015345:Oligonucleotide Probes / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D059007:Polytene Chromosomes / Q000235:genetics*
chemical_list: D017931:DNA Primers; D015345:Oligonucleotide Probes; D004247:DNA