title: Characterization of stress responses of heavy metal and metalloid inducible promoters in synechocystis PCC6803.
abstract: In several biotechnological applications of living bacterial cells with inducible gene expression systems, the extent of overexpression and the specificity to the inducer are key elements. In the present study, we established the concentration ranges of Zn(2+), Ni(2+), Co(2+), AsO(2)(-), and Cd(2+) ions that caused significant activation of the respective promoters of Synechocystis sp. without concomitant unspecific stress responses. The low expression levels can be increased up to 10-100-fold upon treatments with Cd(2+), AsO(2)(-), Zn(2+), and Co(2+) ions and up to 800-fold upon Ni(2+) treatment. These results facilitate the development of conditional gene expression systems in cyanobacteria.
authors.affiliation: Institute of Plant Biology, Biological Research Centre of the Hungarian Academy of Sciences, Temesvari krt. 62, H-6726 Szeged, Hungary.
authors.forename: Barbara
authors.initials: B
authors.lastname: Blasi
authors.forename: Loredana
authors.initials: L
authors.lastname: Peca
authors.forename: Imre
authors.initials: I
authors.lastname: Vass
authors.forename: Peter B
authors.initials: PB
authors.lastname: Kós
mesh_terms: D015964:Gene Expression Regulation, Bacterial; D058955:Metalloids / Q000633:toxicity*; D019216:Metals, Heavy / Q000633:toxicity*; D011401:Promoter Regions, Genetic; D013312:Stress, Physiological; D046939:Synechocystis / Q000187:drug effects* / Q000235:genetics*; D015533:Transcriptional Activation
chemical_list: D058955:Metalloids; D019216:Metals, Heavy