title: Hyphenated techniques and their applications in natural products analysis.
abstract: A technique where a separation technique is coupled with an online spectroscopic detection technology is known as hyphenated technique, e.g., GC-MS, LC-PDA, LC-MS, LC-FTIR, LC-NMR, LC-NMR-MS, and CE-MS. Recent advances in hyphenated analytical techniques have remarkably widened their applications to the analysis of complex biomaterials, especially natural products. This chapter focuses on the applications of hyphenated techniques to pre-isolation and isolation of natural products, dereplication, online partial identification of compounds, chemotaxonomic studies, chemical finger-printing, quality control of herbal products, and metabolomic studies, and presents specific examples. However, a particular emphasis has been given on the hyphenated techniques that involve an LC as the separation tool.
authors.affiliation: Department of Pharmacy, School of Applied Sciences, University of Wolverhampton, Wolverhampton, WV11LY, UK. S.Sarker@wlv.ac.uk
authors.forename: Satyajit D
authors.initials: SD
authors.lastname: Sarker
authors.forename: Lutfun
authors.initials: L
authors.lastname: Nahar
mesh_terms: D001688:Biological Products / Q000032:analysis / Q000302:isolation & purification; D002853:Chromatography, Liquid / Q000379:methods; D019075:Electrophoresis, Capillary; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D029001:Herbal Medicine / Q000592:standards; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D055432:Metabolomics / Q000379:methods; D010936:Plant Extracts / Q000032:analysis / Q000302:isolation & purification*; D010946:Plants, Medicinal / Q000737:chemistry / Q000145:classification; D011786:Quality Control; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods
chemical_list: D001688:Biological Products; D010936:Plant Extracts