title: [Effect of retinoic acid on alkaline phosphatase expression in rat adipose-derived stem cells].
abstract: OBJECTIVE
  To investigate whether adipose-derived stem cells (ADSCs) induced by retinoic acid (RA) in vitro express primordial germ cell marker alkaline phosphatase (ALP) and vasa.
  
  
  METHODS
  ADSCs were isolated from adult female SD rats and cultured in vitro. The third passage of ADSCs was identified by adipogenic differentiation, osteogenic differentiation and cell surface marker detection. The ADSCs were treated with 1×10(-5), 1×10(-6), or 1×10(-7) mol/L RA for 7 or 14 days, and the cellular expression of ALP was detected. vasa mRNA expression in ADSCs treated with 1×10(-5) mol/L RA for 7 days was detected using RT-PCR.
  
  
  RESULTS
  The OD value of ADSCs treated with 1×10(-5), 1×10(-6), or 1×10(-7) mol/L RA was 0.59∓0.04, 0.27∓0.07, and 0.15∓0.03 after a 7-day treatment, and was 0.42∓0.02, 0.34∓0.01, and 0.19∓0.02 after a 14-day treatment, respectively, demonstrating significantly enhanced ALP expression in RA-treated ADSCs compared with that in the control cells (0.07∓0.01 and 0.07∓0.01 at 7 and 14 days, respectively, P<0.01). The ADSCs showed a negative vasa mRNA expression after 1×10(-5) mol/L RA treatment for 7 days.
  
  
  CONCLUSIONS
  RA-induced ADSCs express ALP, a marker of primordial germ cells, but does not express the primordial germ cell marker vasa.
authors.affiliation: Department of Human Anatomy, Southern Medical University, Guangzhou, China. duanhehuan@126.com
authors.forename: Fuhua
authors.initials: F
authors.lastname: Duan
authors.forename: Chun
authors.initials: C
authors.lastname: Yang
authors.forename: Huiying
authors.initials: H
authors.lastname: Yang
authors.forename: Meichun
authors.initials: M
authors.lastname: Yu
authors.forename: Hui
authors.initials: H
authors.lastname: Tao
authors.forename: Wenqin
authors.initials: W
authors.lastname: Zeng
authors.forename: Jingxing
authors.initials: J
authors.lastname: Dai
authors.forename: Lin
authors.initials: L
authors.lastname: Yuan
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D053687:Adult Stem Cells / Q000166:cytology / Q000201:enzymology*; D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D005260:Female; D005854:Germ Cells / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014212:Tretinoin / Q000494:pharmacology*
chemical_list: D014212:Tretinoin; D000469:Alkaline Phosphatase