title: Fabrication of pseudo-ceramide-based lipid microparticles for recovery of skin barrier function.
abstract: The recovery of skin barrier functions was investigated with pseudo-ceramide-based lipid microparticles. The microparticles were prepared by using a fluid bed technique where lipid components (a pseudo-ceramide, cholesterol and a fatty acid) were coated on a sugar seed, and a polymer was subsequently coated on the lipid microparticles. The microparticles contained large amount of pseudo-ceramide, and the pseudo-ceramide was in the form of lamellar structures mixed with other lipid components. In addition, the microparticles were stably dispersed in aqueous media or emulsion systems without any disruption of the microparticles' structures, thereby supplying sufficient amount of the pseudo-ceramide to skins for improving skin barrier functions such as preventing water loss. Such a role of the microparticles was proven by evaluating in vivo the efficacy of the lipid microparticles in reducing a trans-epidermal water loss (TEWL) of impaired murine skins. As a result, the novel pseudo-ceramide-based lipid microparticles for barrier recovery may potentially be applied in the field of dermatology, cosmetics and pharmaceuticals.
authors.affiliation: Amore-Pacific Co. R&D Center, Giheung-gu, Yongin-si, Gyeonggi-Do, Republic of Korea.
authors.forename: Do-Hoon
authors.initials: DH
authors.lastname: Kim
authors.forename: Woo Ram
authors.initials: WR
authors.lastname: Park
authors.forename: Jeong Hwan
authors.initials: JH
authors.lastname: Kim
authors.forename: Eun Chul
authors.initials: EC
authors.lastname: Cho
authors.forename: Eun Jung
authors.initials: EJ
authors.lastname: An
authors.forename: Jin-Woong
authors.initials: JW
authors.lastname: Kim
authors.forename: Seong-Geun
authors.initials: SG
authors.lastname: Oh
mesh_terms: D000287:Administration, Topical; D000818:Animals; D002518:Ceramides / Q000737:chemistry*; D002784:Cholesterol / Q000737:chemistry; D004337:Drug Carriers / Q000008:administration & dosage / Q000138:chemical synthesis*; D004655:Emulsions / Q000737:chemistry; D005260:Female; D051379:Mice; D008812:Mice, Hairless; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D010081:Oxazolone / Q000009:adverse effects; D020127:Recovery of Function / Q000187:drug effects* / Q000502:physiology; D012116:Resins, Plant / Q000737:chemistry; D012867:Skin / Q000187:drug effects* / Q000503:physiopathology; D013229:Stearic Acids / Q000737:chemistry; D014867:Water / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: D002518:Ceramides; D004337:Drug Carriers; D004655:Emulsions; C530540:PC 104; D012116:Resins, Plant; D013229:Stearic Acids; D014867:Water; D010081:Oxazolone; C003934:shellac; C031183:stearic acid; D002784:Cholesterol