title: Proteomics of muscle-specific beef color stability.
abstract: The objective of the present study was to differentiate the sarcoplasmic proteome of color-stable (Longissimus lumborum; LL) and color-labile (Psoas major; PM) beef muscles. LL and PM muscles from seven beef carcasses (24 h post-mortem) were fabricated into 2.54 cm steaks, aerobically packaged, and assigned to refrigerated retail display for 9 days. LL steaks demonstrated greater (P < 0.05) color stability and lower (P < 0.05) lipid oxidation than PM steaks. Proteome analyses identified 16 differentially abundant proteins in LL and PM, including antioxidant proteins and chaperones. Proteins demonstrating positive correlation with redness (aldose reductase, creatine kinase, and β-enolase) and color stability (peroxiredoxin-2, peptide methionine sulfoxide reductase, and heat shock protein-27 kDa) were overabundant in LL, whereas the protein overabundant in PM (mitochondrial aconitase) exhibited negative correlation with redness. The color stability of LL could be attributed to the overabundance of antioxidant proteins and chaperones, and this finding suggests the necessity of developing muscle-specific processing strategies to improve beef color.
authors.affiliation: Department of Animal and Food Sciences, University of Kentucky, Lexington, Kentucky 40546, USA.
authors.forename: Poulson
authors.initials: P
authors.lastname: Joseph
authors.forename: Surendranath P
authors.initials: SP
authors.lastname: Suman
authors.forename: Gregg
authors.initials: G
authors.lastname: Rentfrow
authors.forename: Shuting
authors.initials: S
authors.lastname: Li
authors.forename: Carol M
authors.initials: CM
authors.lastname: Beach
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis; D002417:Cattle; D003116:Color; D004355:Drug Stability; D015227:Lipid Peroxidation; D008460:Meat / Q000032:analysis*; D009124:Muscle Proteins / Q000032:analysis*; D018482:Muscle, Skeletal / Q000737:chemistry*; D040901:Proteomics; D012519:Sarcoplasmic Reticulum / Q000737:chemistry
chemical_list: D000975:Antioxidants; D009124:Muscle Proteins