title: Genetic characterization of Trypanosoma brucei circulating in domestic animals of the Fontem sleeping sickness of Cameroon.
abstract: To improve our knowledge on the transmission dynamics of trypanosomes, Trypanosoma brucei was identified in domestic animals of the Fontem sleeping sickness focus of Cameroon and their genetic characterizations were performed using seven polymorphic microsatellite markers. About 397 domestic animals including 225 pigs, 87 goats, 65 sheep and 20 dogs were sampled. The card agglutination test for trypanosomiasis was positive for 254 (63.98%) animals while the parasitological examinations (thin blood film and capillary tube centrifugation) revealed 86 (21.66%) trypanosome infections. The PCR based method revealed 140 (35.26%) infections of trypanosomes of the subgenus Trypanozoon. The genetic characterization of these 140 positive samples revealed 89 different alleles: 82 in pigs, 72 in goat, 60 in sheep and 48 in dog. Whatever the microsatellite marker used, most of positive samples were amplified. However, the sensitivity (percentage of samples amplified for each marker) of these markers varies significantly between them (χ(2) = 120.32; P < 0.0001). This study showed a high level (80.00%) of mixed genotypes as well as a wide range of T. brucei genotypes circulating in domestic animals of the Fontem sleeping sickness focus of Cameroon. This indicates that several T. brucei genotypes can naturally be transmitted simultaneously to tsetse flies during a single blood meal.
authors.affiliation: Department of Biochemistry, Faculty of Science, PO Box 67, University of Dschang, Dschang, Cameroon. gsimoca@yahoo.fr
authors.forename: Gustave
authors.initials: G
authors.lastname: Simo
authors.forename: Guy Roger
authors.initials: GR
authors.lastname: Njitchouang
authors.forename: Flobert
authors.initials: F
authors.lastname: Njiokou
authors.forename: Gerard
authors.initials: G
authors.lastname: Cuny
authors.forename: Tazoacha
authors.initials: T
authors.lastname: Asonganyi
mesh_terms: D000818:Animals; D002163:Cameroon; D016054:DNA, Protozoan / Q000032:analysis; D004285:Dogs / Q000469:parasitology*; D014644:Genetic Variation; D005838:Genotype; D006041:Goats / Q000469:parasitology*; D007303:Insect Vectors / Q000469:parasitology; D018895:Microsatellite Repeats; D012756:Sheep / Q000469:parasitology*; D013552:Swine / Q000469:parasitology*; D014346:Trypanosoma brucei brucei / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D014352:Trypanosomiasis / Q000469:parasitology* / Q000662:veterinary*; D014370:Tsetse Flies / Q000469:parasitology
chemical_list: D016054:DNA, Protozoan