title: Modification of VTMS hybrid monolith via thiol-ene click chemistry for capillary electrochromatography.
abstract: An n-octadecanethiol (C(18))/3-mercapto-1-propane-sulfonate (MPS) modified organic-inorganic hybrid silica monolithic column possessing vinyl ligands through thiol-ene click chemistry for capillary electrochromatography (CEC) is described. The proposed column is prepared via the sol-gel process by in situ co-condensation using vinyltrimethoxysilane (VTMS) and tetra-methoxysilane (TMOS) as precursors. Examination by SEM shows that the capillary has homogenous macroporous morphology and is well attached to the inner wall of the capillary. The obtained C(18)-MPS-VTMS silica hybrid monolithic column demonstrated an enhanced hydrophilic property and could be applied as a reversed-phase stationary phase in CEC directly. Compared with unmodified VTMS silica hybrid monolithic column, stronger EOF was observed using this monolithic column. VTMS/TOMS ratios in the reaction mixture were varied and 1:3 was found to be optimum. Good separations of benzenes, aromatic amines, acids and peptides were achieved, the lowest plate height of ≈ 3μm was obtained, the peak symmetry range from 0.98 to 1.29. The resulting C(18)-MPS-VTMS silica hybrid monolithic column can be used in different separation modes, including reversed phase mode and ion exchange mode.
authors.affiliation: Key Laboratory of Chemo/Biosensing & Chemometrics, College of Chemistry & Chemical Engineering, Hunan University, Changsha 410082, China.
authors.forename: Keyi
authors.initials: K
authors.lastname: Wang
authors.forename: Yingzhuang
authors.initials: Y
authors.lastname: Chen
authors.forename: Huihui
authors.initials: H
authors.lastname: Yang
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.forename: Lihua
authors.initials: L
authors.lastname: Nie
authors.forename: Shouzhuo
authors.initials: S
authors.lastname: Yao
mesh_terms: D053801:Capillary Electrochromatography / Q000379:methods*; D057930:Click Chemistry / Q000379:methods*; D007475:Ion Exchange Resins; D012821:Silanes / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry; D014753:Vinyl Compounds / Q000737:chemistry*
chemical_list: D007475:Ion Exchange Resins; D012821:Silanes; D013438:Sulfhydryl Compounds; D014753:Vinyl Compounds; C529208:trimethoxyvinylsilane