title: Separation of natural products by countercurrent chromatography.
abstract: Countercurrent Chromatography (CCC) provides the natural product chemist with a high-resolution separatory method, which is uniquely applicable to sensitive (unstable) compounds and which allows virtually quantitative recovery of the load sample. Different instruments use different means of retaining a stationary liquid phase. The solvent system (SS) can be chosen to optimize the separatory power and the number of systems available is limitless. Several examples are provided to illustrate the power of the method and to guide the chemist in choice of an appropriate SS.
authors.affiliation: Department of Medicinal Chemistry and Pharmacognosy, School of Pharmacy, University of Illinois at Chicago, Chicago, IL, USA. mcalpine@uic.edu
authors.forename: James B
authors.initials: JB
authors.lastname: McAlpine
authors.forename: J Brent
authors.initials: JB
authors.lastname: Friesen
authors.forename: Guido F
authors.initials: GF
authors.lastname: Pauli
mesh_terms: D001688:Biological Products / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D002498:Centrifugation / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D003377:Countercurrent Distribution / Q000379:methods; D006863:Hydrogen-Ion Concentration; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D010944:Plants / Q000737:chemistry; D012997:Solvents / Q000737:chemistry
chemical_list: D001688:Biological Products; D010936:Plant Extracts; D012997:Solvents