title: Urinary metabonomic study using a CUMS rat model of depression.
abstract: Chronic unpredictable mild stress (CUMS) is a well-validated model of depression. In this study, a urinary metabonomics method based on the NMR spectrometry was used to study the metabolic perturbation in CUMS-induced rat depression model. With pattern recognition analysis, a clear separation of CUMS rats and healthy controls was achieved, and nine endogenous metabolites contributing to the separation were identified. CUMS-treated rats were characterized by the increase of glycine, pyruvate, glutamine, and asparagines, as well as the decrease of 2-oxoglutarate, dimethylglycine, citrate, succinate, and acetate. The urinary biochemical changes related to the metabolic disturbance in CUMS induced depression, and the possible correlations with live qi stagnation in traditional Chinese medicine are discussed. The work shows that CUMS is a reliable model for studying depression, and the noninvasive urinary metabolomic method is a valuable tool to investigate the biochemical pertubations in depression as an early diagnostic means.
authors.affiliation: Modern Research Center for Traditional Chinese Medicine of Shanxi University, Taiyuan, China.
authors.forename: Xiao-Jie
authors.initials: XJ
authors.lastname: Liu
authors.forename: Zhen-Yu
authors.initials: ZY
authors.lastname: Li
authors.forename: Zhong-Feng
authors.initials: ZF
authors.lastname: Li
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Gao
authors.forename: Yu-Zhi
authors.initials: YZ
authors.lastname: Zhou
authors.forename: Hai-Feng
authors.initials: HF
authors.lastname: Sun
authors.forename: Li-Zeng
authors.initials: LZ
authors.lastname: Zhang
authors.forename: Xiao-Qing
authors.initials: XQ
authors.lastname: Guo
authors.forename: Guan-Hua
authors.initials: GH
authors.lastname: Du
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Qin
mesh_terms: D000143:Acids / Q000378:metabolism / Q000652:urine*; D000588:Amines / Q000378:metabolism / Q000652:urine*; D000596:Amino Acids / Q000378:metabolism / Q000652:urine*; D000818:Animals; D001522:Behavior, Animal; D003863:Depression / Q000378:metabolism / Q000652:urine*; D004195:Disease Models, Animal; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013312:Stress, Physiological
chemical_list: D000143:Acids; D000588:Amines; D000596:Amino Acids