title: Protection of pigs against genital Chlamydia trachomatis challenge by parenteral or mucosal DNA immunization.
abstract: The current study evaluates combined aerosol-vaginal delivery of a MOMP-based Chlamydia trachomatis (serovar E) DNA vaccine in a pig genital challenge model. Most non-replicating antigens are rather poor mucosal immunogens in comparison to replicating antigens. Therefore, a mucosal administered DNA vaccine, which actually mimics a live vaccine, could be promising. Protection was promoted by plasmids encoding the porcine granulocyte macrophage-colony stimulating factor (pcDNA3.1zeo::GM-CSF), the Escherichia coli thermo-labile enterotoxin (LT) subunit A (plasmid PJV2004::LTa) and subunit B (plasmid PJV2005::LTb). Mucosal C. trachomatis DNA vaccination induced significant protection against genital C. trachomatis challenge although the infection could not be eradicated. Intradermal immunization was significantly less efficient in protecting experimentally infected pigs. Protection was correlated with efficient T cell priming and significantly higher serum IgA titers following primo vaccination.
authors.affiliation: Department of Molecular Biotechnology, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium. Katelijn.Schautteet@UGent.be
authors.forename: Katelijn
authors.initials: K
authors.lastname: Schautteet
authors.forename: Evelien
authors.initials: E
authors.lastname: De Clercq
authors.forename: Yannick
authors.initials: Y
authors.lastname: Jönsson
authors.forename: Stefanie
authors.initials: S
authors.lastname: Lagae
authors.forename: Koen
authors.initials: K
authors.lastname: Chiers
authors.forename: Eric
authors.initials: E
authors.lastname: Cox
authors.forename: Daisy
authors.initials: D
authors.lastname: Vanrompay
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000235:genetics; D000280:Administration, Inhalation; D000282:Administration, Intravaginal; D000818:Animals; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology*; D002692:Chlamydia trachomatis / Q000235:genetics / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D018928:Immunity, Mucosal; D007114:Immunization / Q000379:methods*; D008219:Lymphogranuloma Venereum / Q000276:immunology / Q000517:prevention & control*; D010957:Plasmids / Q000008:administration & dosage; D013552:Swine; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D001428:Bacterial Vaccines; D019444:Vaccines, DNA