title: Cargo ubiquitination is essential for multivesicular  body intralumenal vesicle formation.
abstract: The efficient formation of a variety of transport vesicles is influenced by the presence of cargo, suggesting that cargo itself might have a defining role in vesicle biogenesis. However, definitive in vivo experiments supporting this concept are lacking, as it is difficult to eliminate endogenous cargo. The Endosomal Sorting Complexes Required for Transport (ESCRT) apparatus sorts ubiquitinated membrane proteins into endosomal intralumenal vesicles (ILVs) that accumulate within multivesicular bodies. Here we show that cargo ubiquitination is required for effective recruitment of the ESCRT machinery onto endosomal membranes and for the subsequent formation of ILVs.
authors.affiliation: Molecular Physiology and Biophysics, University of Iowa, Iowa City, Iowa 52246, USA.
authors.forename: Chris
authors.initials: C
authors.lastname: MacDonald
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Buchkovich
authors.forename: Daniel K
authors.initials: DK
authors.lastname: Stringer
authors.forename: Scott D
authors.initials: SD
authors.lastname: Emr
authors.forename: Robert C
authors.initials: RC
authors.lastname: Piper
mesh_terms: D056827:Endosomal Sorting Complexes Required for Transport / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008954:Models, Biological; D057146:Multivesicular Bodies / Q000378:metabolism* / Q000648:ultrastructure; D021381:Protein Transport; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism* / Q000648:ultrastructure; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D057149:Ubiquitinated Proteins / Q000378:metabolism; D054875:Ubiquitination
chemical_list: D056827:Endosomal Sorting Complexes Required for Transport; C464563:Hse1 protein, S cerevisiae; D018160:Receptors, Cytoplasmic and Nuclear; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D057149:Ubiquitinated Proteins; D049452:Green Fluorescent Proteins