title: Molecular cloning of chicken interleukin-5 receptor α-chain and analysis of its binding specificity.
abstract: Interaction between interleukin (IL)-5 and its receptor (IL-5R) is important for the regulation of immunity against worm infections, allergic reactions and B cell response in mammals. In this study, we identified a full-length cDNA encoding chicken IL-5R α-chain (chIL-5Rα). The deduced amino acid sequence showed 41-43% identity to mammalian homologues. It has four well-conserved cysteines and a WSXWS motif in the extracellular region, and a PPXP motif in the cytoplasmic region. Quantitative RT-PCR analysis revealed that chIL-5Rα mRNA expression was markedly high in bone marrow and relatively high in spleen and lung. Recombinant proteins of soluble chIL-5Rα and cytokines (artificially produced chIL-5 (achIL-5) and another IL-5-like molecule KK34) were expressed by 293F cells to examine the cytokine-receptor interactions. Interaction assay using a Biacore biosensor showed that chIL-5Rα has the capability to bind with monomeric achIL-5, but not with KK34. In conclusion, chicken has an IL-5Rα homologue but KK34 does not complement the IL-5/IL-5R system.
authors.affiliation: Department of Immunobiology, Graduate School of Biosphere Science, Hiroshima University, 1-4-4 Kagamiyama, Higashi-Hiroshima 739-8528, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Fukushima
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Miyai
authors.forename: Manami
authors.initials: M
authors.lastname: Kumagae
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Horiuchi
authors.forename: Shuichi
authors.initials: S
authors.lastname: Furusawa
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002645:Chickens / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D005260:Female; D006801:Humans; D015848:Interleukin-5 / Q000235:genetics / Q000378:metabolism; D053666:Interleukin-5 Receptor alpha Subunit / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D015848:Interleukin-5; D053666:Interleukin-5 Receptor alpha Subunit; D020033:Protein Isoforms; D012333:RNA, Messenger; D011994:Recombinant Proteins