title: Label-free 3D imaging of microstructure, blood, and lymphatic vessels within tissue beds in vivo.
abstract: This Letter reports the use of an ultrahigh resolution optical microangiography (OMAG) system for simultaneous 3D imaging of microstructure and lymphatic and blood vessels without the use of an exogenous contrast agent. An automatic algorithm is developed to segment the lymphatic vessels from the microstructural images based on the fact that the lymph fluid is optically transparent. An OMAG system is developed that utilizes a broadband supercontinuum light source, providing an axial resolution of 2.3 μm and lateral resolution of 5.8 μm, capable of resolving the capillary vasculature and lymphatic vessels innervating microcirculatory tissue beds. Experimental demonstration is performed by showing detailed 3D lymphatic and blood vessel maps, coupled with morphology, within mouse ears in vivo.
authors.affiliation: Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA.
authors.forename: Zhongwei
authors.initials: Z
authors.lastname: Zhi
authors.forename: Yeongri
authors.initials: Y
authors.lastname: Jung
authors.forename: Ruikang K
authors.initials: RK
authors.lastname: Wang
mesh_terms: D000792:Angiography / Q000379:methods*; D000818:Animals; D001808:Blood Vessels; D054644:Ear Auricle / Q000098:blood supply / Q000276:immunology; D021621:Imaging, Three-Dimensional / Q000379:methods*; D042601:Lymphatic Vessels / Q000000981:diagnostic imaging*; D008220:Lymphography; D051379:Mice; D041623:Tomography, Optical Coherence