title: Studies of ciprofloxacin encapsulation on alginate/pectin matrixes and its relationship with biodisponibility.
abstract: Screening of ciprofloxacin (Cip) with selected biopolymers brings about 90% antibiotic interactions with a coacervate composed of alginate/high metoxylated pectin in 2:1 ratio. Fourier transform infrared spectroscopy analysis provides information about the nature of this interaction, revealing ionic and hydrophobic patterns among the molecules. Alginate/high methoxylated pectin gel microspheres developed by ionic gelation encapsulates 46.8 ± 5.0% Cip. The gel matrix can release Cip in a sustained manner, releasing 42.7 ± 0.2% in 2 h under simulated stomach pH conditions, and 83.3 ± 1.1% Cip release in 80 mM phosphate at pH = 7.40 (intestinal). The increase of sodium chloride from 50 to 200 mM implies a Cip release from 69.0 ± 1.5% to 95.1 ± 3.6% respectively in 2 h. Scanning electron microscopy revealed the cohesive effect of HM pectin over alginate molecules on the microsphere surface. Those results guarantee all Cip contained in the alginate/HM pectin microspheres could be released in an established kinetic profile along the gastrointestinal tract, avoiding the Cip undesirable side effects during absorption.
authors.affiliation: Nanobiomaterials Laboratory, CINDEFI-CONICET, CCT La Plata-School of Sciences, Universidad Nacional de La Plata, La Plata 1900, Argentina.
authors.forename: Germán A
authors.initials: GA
authors.lastname: Islan
authors.forename: Ignacio Pérez
authors.initials: IP
authors.lastname: de Verti
authors.forename: Sergio G
authors.initials: SG
authors.lastname: Marchetti
authors.forename: Guillermo R
authors.initials: GR
authors.lastname: Castro
mesh_terms: D000284:Administration, Oral; D000464:Alginates / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry* / Q000378:metabolism*; D032701:Biomimetics; D002214:Capsules; D002939:Ciprofloxacin / Q000008:administration & dosage / Q000737:chemistry* / Q000378:metabolism*; D004337:Drug Carriers / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007413:Intestinal Mucosa / Q000378:metabolism; D007700:Kinetics; D010368:Pectins / Q000737:chemistry*
chemical_list: D000464:Alginates; D000900:Anti-Bacterial Agents; D002214:Capsules; D004337:Drug Carriers; D006603:Hexuronic Acids; D002939:Ciprofloxacin; D010368:Pectins; D020723:Glucuronic Acid