title: [Effects of pseudolaric acid B on apoptosis of bladder cancer cell 5637].
abstract: OBJECTIVE
  To investigate the apoptosis of bladder cancer cell 5637 induced by pseudolaric acid B in vitro and its mechanism.
  
  
  METHODS
  The cell proliferation was detected by MTT assay;the cell cycle was measured by flow cytometry; the cell apoptosis was observed by flow cytometry with Annexin V-FITC/PI double staining; the expressions of survivin protein and caspase-3 protein were detected by Western blot assay.
  
  
  RESULTS
  It showed that pseudolaric acid B remarkably induced apoptosis of 5637 cell line. Moreover, pseudolaric acid B suppressed survivin and up-regulated caspase-3 expression.
  
  
  CONCLUSIONS
  Pseudolaric acid B inhibits the proliferation and induces the apoptosis of 5637 cells. The molecular mechanism of pseudolaric acid B inducing the apoptosis of 5637 cells may be associated with its action of down-regulating the expression of survivin, and up-regulating the expression of caspase-3.
authors.affiliation: Department of Urologic Surgery, First Affiliated Hospital, Liaoning Medical University, Jinzhou 121000, China. qugengqing@163.com
authors.forename: Gengqing
authors.initials: G
authors.lastname: Qu
authors.forename: Yuwen
authors.initials: Y
authors.lastname: Sheng
authors.forename: Bo
authors.initials: B
authors.lastname: Chen
authors.forename: Ben
authors.initials: B
authors.lastname: Liu
authors.forename: Lichun
authors.initials: L
authors.lastname: Yu
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000032:analysis; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004224:Diterpenes / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000032:analysis; D000077022:Survivin; D001749:Urinary Bladder Neoplasms / Q000188:drug therapy* / Q000473:pathology
chemical_list: C107489:BIRC5 protein, human; D004224:Diterpenes; D004365:Drugs, Chinese Herbal; D051034:Inhibitor of Apoptosis Proteins; D000077022:Survivin; C058391:pseudolaric acid B; D053148:Caspase 3