title: Effect of butyrate on aromatase cytochrome P450 levels in HT29, DLD-1 and LoVo colon cancer cells.
abstract: Epidemiological studies suggest that colonic production of butyrate and estrogen may be involved in human susceptibility to colorectal cancer (CRC). Estrone (E1) can be produced by the aromatase pathway during the conversion of androstenedione (A) to E1. Therefore, we studied the effect of sodium butyrate (NaBu) on the CYP19A1 transcript and protein levels and on the conversion of A to E1 in HT29, DLD-1 and LoVo CRC cells. We found that NaBu significantly downregulated CYP19A1 transcript and protein levels, a phenomenon that was associated with reduced conversion of A to E1 in HT29, DLD-1 and LoVo cells. Our studies demonstrated that, although butyrate exhibited a protective role in CRC development, this compound may reduce aromatase activity and the production of E1 in colon cancer cells.
authors.affiliation: Department of Biochemistry and Molecular Biology, Poznań University of Medical Sciences, 6, Święcickiego Street, 60781 Poznań, Poland.
authors.forename: Agnieszka Anna
authors.initials: AA
authors.lastname: Rawłuszko
authors.forename: Sylwia
authors.initials: S
authors.lastname: Sławek
authors.forename: Armin
authors.initials: A
authors.lastname: Gollogly
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Szkudelska
authors.forename: Paweł Piotr
authors.initials: PP
authors.lastname: Jagodziński
mesh_terms: D000735:Androstenedione / Q000378:metabolism; D001141:Aromatase / Q000235:genetics / Q000378:metabolism*; D002087:Butyrates / Q000494:pharmacology*; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000378:metabolism*; D015536:Down-Regulation / Q000187:drug effects; D004970:Estrone / Q000378:metabolism*; D006801:Humans
chemical_list: D002087:Butyrates; D004970:Estrone; D000735:Androstenedione; D001141:Aromatase; C561410:CYP19A1 protein, human