title: [Mouse melanoma cell line B16F10-derived conditioned medium inhibits  sodium L-ascorbate-induced B16F10 cell apoptosis].
abstract: OBJECTIVE
  To investigate the effect of mouse melanoma cell line B16F10-derived conditioned medium on the apoptosis of B16F10 cells.
  
  
  METHODS
  B16F10 cells were cultured in high-glucose DMEM in the presence of 10% fetal bovine serum, and upon cell confluence, the growth medium was replaced with serum-free high-glucose DMEM. After 8 h, the medium was collected and infiltrated to serve as the conditioned medium. B16F10 cells cultured in normal growth medium or the conditioned medium were exposed to 10 mmol/L sodium L-ascorbate, and the cell apoptosis was analyzed. The ingredients in the conditioned medium with relative molecular mass less or more than 5 000 were extracted to assess their effect on sodium L-ascorbate-induced cell apoptosis.
  
  
  RESULTS
  The conditioned medium for B16F10 cells significantly inhibited cell apoptosis induced by sodium L-ascorbate, and the effective ingredients in the medium showed a relative molecular mass below 5,000.
  
  
  CONCLUSIONS
  Mouse melanoma cell line B16F10-derived conditioned medium can suppress sodium L-ascorbate- induced apoptosis of B16F10 cells, and the ingredients with relative molecular mass less than 5 000 are responsible for this effect.
authors.affiliation: Guangdong Women and Children's Hospital, Guangzhou, China. yxhshy52@163.com
authors.forename: Xuhui
authors.initials: X
authors.lastname: Yang
authors.forename: Tian
authors.initials: T
authors.lastname: Xia
authors.forename: Weihua
authors.initials: W
authors.lastname: Yu
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Lu
authors.forename: Peng
authors.initials: P
authors.lastname: Xiang
authors.forename: Feng
authors.initials: F
authors.lastname: He
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001205:Ascorbic Acid / Q000037:antagonists & inhibitors*; D045744:Cell Line, Tumor; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D008546:Melanoma, Experimental / Q000473:pathology*; D051379:Mice
chemical_list: D017077:Culture Media, Conditioned; D001205:Ascorbic Acid