title: Identification and sensitivity of two rare fungal species isolated from two patients with Fusarium keratomycosis.
abstract: We report two cases of Fusarium keratomycosis in which molecular analysis was used to identify two rare causative Fusarium species. A 65-year-old woman was diagnosed with Fusarium keratomycosis caused by F. equiseti, confirmed by sequencing the internal transcribed spacer (ITS) region and morphological characteristics. She was initially treated with topical corticosteroid but the keratitis did not improve. Even after aggressive antifungal treatment, there was a severe reduction of vision. In the end, the eye perforated. Our second case was a 75-year-old man who had diabetes mellitus. He developed keratitis after his cornea was injured by corn stalks. The ITS region identified the causative organism as F. verticillioides. Unlike the first case, he had good visual recovery after early antifungal therapy. Our findings demonstrate that both ITS and morphological characteristics can be used to identify the exact Fusarium species causing Fusarium keratomycosis. This allowed us to determine the sensitivity of these species to antifungal drugs that can be used to treat patients with these species of Fusarium.
authors.affiliation: Department of Ophthalmology, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu 501-1194, Japan. mochi-gif@umin.ac.jp
authors.forename: Kiyofumi
authors.initials: K
authors.lastname: Mochizuki
authors.forename: Ikumi
authors.initials: I
authors.lastname: Shiraki
authors.forename: Hiroki
authors.initials: H
authors.lastname: Murase
authors.forename: Kiyofumi
authors.initials: K
authors.lastname: Ohkusu
authors.forename: Kazuko
authors.initials: K
authors.lastname: Nishimura
mesh_terms: D000368:Aged; D000935:Antifungal Agents / Q000494:pharmacology / Q000627:therapeutic use; D003315:Cornea / Q000382:microbiology / Q000473:pathology; D021901:DNA, Intergenic / Q000235:genetics; D015821:Eye Infections, Fungal / Q000382:microbiology*; D005260:Female; D060585:Fusariosis / Q000382:microbiology*; D005670:Fusarium / Q000145:classification* / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D007634:Keratitis / Q000382:microbiology*; D008297:Male; D008826:Microbial Sensitivity Tests
chemical_list: D000935:Antifungal Agents; D021901:DNA, Intergenic