title: Different localization patterns of anthocyanin species in the pericarp of black rice revealed by imaging mass spectrometry.
abstract: Black rice (Oryza sativa L. Japonica) contains high levels of anthocyanins in the pericarp and is considered an effective health-promoting food. Several studies have identified the molecular species of anthocyanins in black rice, but information about the localization of each anthocyanin species is limited because methodologies for investigating the localization such as determining specific antibodies to anthocyanin, have not yet been developed Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) is a suitable tool for investigating the localization of metabolites. In this study, we identified 7 species of anthocyanin monoglycosides and 2 species of anthocyanin diglycosides in crude extracts from black rice by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) analysis. We also analyzed black rice sections by MALDI-IMS and found 2 additional species of anthocyanin pentosides and revealed different localization patterns of anthocyanin species composed of different sugar moieties. Anthocyanin species composed of a pentose moiety (cyanidin-3-O-pentoside and petunidin-3-O-pentoside) were localized in the entire pericarp, whereas anthocyanin species composed of a hexose moiety (cyanidin-3-O-hexoside and peonidin-3-O-hexoside) were focally localized in the dorsal pericarp. These results indicate that anthocyanin species composed of different sugar moieties exhibit different localization patterns in the pericarp of black rice. This is the first detailed investigation into the localization of molecular species of anthocyanins by MALDI-IMS.
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agriculture, Kinki University, Nara, Japan.
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Yoshimura
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Zaima
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Moriyama
authors.forename: Yukio
authors.initials: Y
authors.lastname: Kawamura
mesh_terms: D000872:Anthocyanins / Q000737:chemistry / Q000378:metabolism*; D001692:Biological Transport; D035264:Flowers / Q000378:metabolism*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D007477:Ions; D012275:Oryza / Q000378:metabolism*; D010858:Pigmentation / Q000502:physiology*; D010936:Plant Extracts / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D000872:Anthocyanins; D007477:Ions; D010936:Plant Extracts