title: De novo lipogenesis in adipose tissue is associated with course of morbid obesity after bariatric surgery.
abstract: OBJECTIVE
  De novo lipogenesis is involved in fatty acid biosynthesis and could be involved in the regulation of the triglyceride storage capacity of adipose tissue. However, the association between lipogenic and lipolytic genes and the evolution of morbidly obese subjects after bariatric surgery remains unknown. In this prospective study we analyze the association between the improvement in the morbidly obese patients as a result of bariatric surgery and the basal expression of lipogenic and lipolytic genes.
  
  
  METHODS
  We study 23 non diabetic morbidly obese patients who were studied before and 7 months after bariatric surgery. Also, we analyze the relative basal mRNA expression levels of lipogenic and lipolytic genes in epiploic visceral adipose tissue (VAT) and abdominal subcutaneous adipose tissue (SAT).
  
  
  RESULTS
  When the basal acetyl-CoA carboxylase 1 (ACC1), acetyl-CoA synthetase 2 (ACSS2) and ATP citrate lyase (ACL) expression in SAT was below percentile-50, there was a greater decrease in weight (P = 0.006, P = 0.034, P = 0.026), body mass index (P = 0.008, P = 0.033, P = 0.034) and hip circumference (P = 0.033, P = 0.021, P = 0.083) after bariatric surgery. In VAT, when the basal ACSS2 expression was below percentile-50, there was a greater decrease in hip circumference (P = 0.006). After adjusting for confounding variables in logistic regression models, only the morbidly obese patients with SAT or VAT ACSS2 expression ≥ P50 before bariatric surgery had a lower percentage hip circumference loss (<P50) after bariatric surgery (SAT: P = 0.039; VAT: P = 0.033).
  
  
  CONCLUSIONS
  A lower basal ACSS2, ACC1 and ACL expression, genes involved in de novo lipogenesis, is associated with a better evolution of anthropometric variables after bariatric surgery. Thus, the previous state of the pathways involved in fatty acid metabolism may have repercussions on the improvement of these patients.
authors.affiliation: CIBERDEM, Hospital Universitari Joan XXIII, IISPV, Tarragona, Spain.
authors.forename: Lourdes
authors.initials: L
authors.lastname: Garrido-Sánchez
authors.forename: Joan
authors.initials: J
authors.lastname: Vendrell
authors.forename: Diego
authors.initials: D
authors.lastname: Fernández-García
authors.forename: Victoria
authors.initials: V
authors.lastname: Ceperuelo-Mallafré
authors.forename: Matilde R
authors.initials: MR
authors.lastname: Chacón
authors.forename: Luis
authors.initials: L
authors.lastname: Ocaña-Wilhelmi
authors.forename: Juan
authors.initials: J
authors.lastname: Alcaide
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Tinahones
authors.forename: Eduardo
authors.initials: E
authors.lastname: García-Fuentes
mesh_terms: D001275:ATP Citrate (pro-S)-Lyase / Q000235:genetics; D000103:Acetyl-CoA Carboxylase / Q000235:genetics; D000123:Acetyltransferases / Q000235:genetics; D000273:Adipose Tissue / Q000502:physiology*; D000328:Adult; D000886:Anthropometry / Q000379:methods; D050110:Bariatric Surgery / Q000379:methods*; D005260:Female; D006801:Humans; D050152:Intra-Abdominal Fat / Q000378:metabolism; D050155:Lipogenesis; D008066:Lipolysis; D008297:Male; D008875:Middle Aged; D009767:Obesity, Morbid / Q000235:genetics / Q000628:therapy*; D012333:RNA, Messenger / Q000378:metabolism; D050151:Subcutaneous Fat / Q000378:metabolism; D016896:Treatment Outcome
chemical_list: D012333:RNA, Messenger; D000123:Acetyltransferases; C055210:aminoglycoside N1-acetyltransferase; D001275:ATP Citrate (pro-S)-Lyase; C520105:ACACB protein, human; D000103:Acetyl-CoA Carboxylase