title: Anti-LRP/LR-specific antibody IgG1-iS18 significantly reduces adhesion and invasion of metastatic lung, cervix, colon and prostate cancer cells.
abstract: The 37-kDa/67-kDa laminin receptor [laminin receptor precursor/high-affinity laminin receptor (LRP/LR)] is thought to play a major role in invasion and adhesion, key components of metastatic cancer. Lung cancer, cervical cancer, colon cancer and prostate cancer are among the top 10 cancer types worldwide. Here, we report that LRP/LR levels on the surface of lung cancer cells, cervical cancer cells, colon cancer cells and prostate cancer cells are significantly increased compared to non-tumorigenic fibroblasts. Adhesion of lung cancer cells, cervical cancer cells, colon cancer cells and prostate cancer cells to laminin-1 is significantly reduced, employing the anti-LRP/LR-specific antibody IgG1-iS18. Invasion of these cell lines into the Matrigel™ matrix was significantly impeded with IgG1-iS18. The Pearson's correlation coefficient proves a correlation between LRP/LR cell-surface levels and invasion potential, as well as adhesion and invasion, respectively. Our findings suggest that IgG1-iS18 antibody might act as alternative therapeutic tool for treatment of various metastatic cancer types.
authors.affiliation: School of Molecular and Cell Biology, University of the Witwatersrand, Private Bag 3, Wits 2050, Johannesburg, The Republic of South Africa.
authors.forename: Aadilah
authors.initials: A
authors.lastname: Omar
authors.forename: Uwe
authors.initials: U
authors.lastname: Reusch
authors.forename: Stefan
authors.initials: S
authors.lastname: Knackmuss
authors.forename: Melvyn
authors.initials: M
authors.lastname: Little
authors.forename: Stefan F T
authors.initials: SF
authors.lastname: Weiss
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000918:Antibody Specificity; D002448:Cell Adhesion / Q000276:immunology*; D002460:Cell Line; D045744:Cell Line, Tumor; D005260:Female; D005347:Fibroblasts / Q000276:immunology / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D008297:Male; D051379:Mice; D041681:NIH 3T3 Cells; D009361:Neoplasm Invasiveness; D009369:Neoplasms / Q000276:immunology* / Q000473:pathology* / Q000628:therapy; D011956:Receptors, Cell Surface / Q000276:immunology / Q000378:metabolism; D017474:Receptors, Laminin / Q000276:immunology* / Q000378:metabolism
chemical_list: D000888:Antibodies, Anti-Idiotypic; D007074:Immunoglobulin G; D011956:Receptors, Cell Surface; D017474:Receptors, Laminin