title: Fluorescence-based multiplex protein detection using optically encoded microbeads.
abstract: Potential utilization of proteins for early detection and diagnosis of various diseases has drawn considerable interest in the development of protein-based multiplex detection techniques. Among the various techniques for high-throughput protein screening, optically-encoded beads combined with fluorescence-based target monitoring have great advantages over the planar array-based multiplexing assays. This review discusses recent developments of analytical methods of screening protein molecules on microbead-based platforms. These include various strategies such as barcoded microbeads, molecular beacon-based techniques, and surface-enhanced Raman scattering-based techniques. Their applications for label-free protein detection are also addressed. Especially, the optically-encoded beads such as multilayer fluorescence beads and SERS-encoded beads are successful for generating a large number of coding.
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul 151-747, Korea.
authors.forename: Bong-Hyun
authors.initials: BH
authors.lastname: Jun
authors.forename: Homan
authors.initials: H
authors.lastname: Kang
authors.forename: Yoon-Sik
authors.initials: YS
authors.lastname: Lee
authors.forename: Dae Hong
authors.initials: DH
authors.lastname: Jeong
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D008403:Mass Screening / Q000379:methods; D008863:Microspheres; D015336:Molecular Probe Techniques; D015335:Molecular Probes / Q000737:chemistry*; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry*; D013059:Spectrum Analysis, Raman
chemical_list: D055910:Antibodies, Immobilized; D005456:Fluorescent Dyes; D015335:Molecular Probes; D011506:Proteins