title: [Clinical significance of monitoring BK polyomavirus in patients after hematopoietic stem cell transplantation].
abstract: This study was aimed to establish a method for rapid detecting BK polyomavirus (BKV) and to investigate the feasibility and value used in leukemia patients undergoing hematopoietic stem cell transplantation. Primers were designed according to BKV gene sequence; the quantitative standards for BKV and a real-time fluorescent quantitative PCR for BKV were established. The BKV level in urine samples from 36 patients after hematopoietic stem cell transplantation were detected by established method. The results showed that the standard of reconstructed plasmid and real time fluorescent quantitative PCR method were successfully established, its good specificity, sensitivity and stability were confirmed by experiments. BKV was found in 55.56% of urine samples, and the BKV load in urine was 2.46 × 10(4) - 7.8 × 10(9) copy/ml. It is concluded that the establishment of real-time fluorescent quantitative PCR for BKV detection provides a method for early diagnosis of the patients with hemorrhagic cystitis after hematopoietic stem cell transplantation.
authors.affiliation: Department of Hematology, Southern Medical University, Guangzhou, Guangdong Province, China.
authors.forename: Chang-Xin
authors.initials: CX
authors.lastname: Yin
authors.forename: Qian-Li
authors.initials: QL
authors.lastname: Jiang
authors.forename: Han
authors.initials: H
authors.lastname: He
authors.forename: Guo-Pan
authors.initials: GP
authors.lastname: Yu
authors.forename: Yue
authors.initials: Y
authors.lastname: Xu
authors.forename: Fan-Yi
authors.initials: FY
authors.lastname: Meng
authors.forename: Mo
authors.initials: M
authors.lastname: Yang
mesh_terms: D000293:Adolescent; D000328:Adult; D001739:BK Virus / Q000302:isolation & purification*; D016022:Case-Control Studies; D003556:Cystitis / Q000517:prevention & control* / Q000821:virology; D017931:DNA Primers; D004279:DNA, Viral / Q000652:urine*; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation; D006470:Hemorrhage / Q000517:prevention & control / Q000821:virology; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D027601:Polyomavirus Infections / Q000175:diagnosis* / Q000821:virology; D019562:Viral Load; D055815:Young Adult
chemical_list: D017931:DNA Primers; D004279:DNA, Viral