title: Molecular characterization of the Andean blackberry, Rubus  glaucus, using SSR markers.
abstract: The species Rubus glaucus, also known as the  Andean or "Castilla" blackberry, is one of nine edible species of this genus  that grow naturally in Central and South America. In Colombia, this species is  the most important of all Rubus species for agricultural and commercial  purposes. We used 20 SSRs developed for other Rubus species to  characterize 44 Colombian R. glaucus genotypes, collected from eight  different departments, and to look for molecular differences between thornless  and thorny cultivated blackberries. Eighty-two bands were obtained from 28  loci. The genotypes were classified into eight populations, corresponding to  collection sites. The mean number of polymorphic alleles per locus in all  populations and genotypes ranged from 1.857 to 2.393. Samples collected from  Valle del Cauca, Quindío, Caldas, and Risaralda departments had the highest  heterozygosity values. The finding of exclusive bands from R. glaucus genotypes  from Valle del Cauca, Quindío, and Caldas demonstrates genetic and molecular  differentiation between thorny and thornless Andean blackberries.
authors.affiliation: Laboratorio de Biotecnología Vegetal, Facultad de Ciencias Ambientales, Universidad Tecnológica de Pereira, Pereira, Colombia. mlmarulanda@utp.edu.co
authors.forename: M
authors.initials: M
authors.lastname: Marulanda
authors.forename: A M
authors.initials: AM
authors.lastname: López
authors.forename: M
authors.initials: M
authors.lastname: Uribe
mesh_terms: D000383:Agriculture; D000483:Alleles; D032461:Chromosomes, Plant / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics*; D005838:Genotype; D018895:Microsatellite Repeats / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D027824:Rosaceae / Q000235:genetics*
chemical_list: D018744:DNA, Plant