title: Nanopore detection of single molecule RNAP-DNA transcription complex.
abstract: In the past decade, a number of single-molecule methods have been developed with the aim of investigating single protein and nucleic acid interactions. For the first time we use solid-state nanopore sensing to detect a single E. coli RNAP-DNA transcription complex and single E. coli RNAP enzyme. On the basis of their specific conductance translocation signature, we can discriminate and identify between those two types of molecular translocations and translocations of bare DNA. This opens up a new perspectives for investigating transcription processes at the single-molecule level.
authors.affiliation: Laboratory of Nanoscale Biology, Institute of Bioengineering, School of Engineering, EPFL, 1015 Lausanne, Switzerland.
authors.forename: C
authors.initials: C
authors.lastname: Raillon
authors.forename: P
authors.initials: P
authors.lastname: Cousin
authors.forename: F
authors.initials: F
authors.lastname: Traversi
authors.forename: E
authors.initials: E
authors.lastname: Garcia-Cordero
authors.forename: N
authors.initials: N
authors.lastname: Hernandez
authors.forename: A
authors.initials: A
authors.lastname: Radenovic
mesh_terms: D001665:Binding Sites; D004269:DNA, Bacterial / Q000737:chemistry* / Q000648:ultrastructure*; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry* / Q000648:ultrastructure*; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D016062:Porosity; D011485:Protein Binding; D015533:Transcriptional Activation
chemical_list: D004269:DNA, Bacterial; D012321:DNA-Directed RNA Polymerases