title: Human PIH1 associates with histone H4 to mediate the glucose-dependent enhancement of pre-rRNA synthesis.
abstract: Ribosome biogenesis is critical in the growth of eukaryotic cells, in which the synthesis of precursor ribosomal RNA is the first and rate-limiting step. Here, we show that human PIH1 domain-containing protein 1 (PIH1) interacts directly with histone H4 and recruits the Brg1-SWI/SNF complex via SNF5 to human rRNA genes. This process is likely involved in PIH1-dependent DNase I-hypersensitive chromatin remodeling at the core promoter of the rRNA genes. PIH1 mediates the occupancy of not only the Brg1 complex but also the Pol I complex at the core promoter and enhances transcription initiation of rRNA genes. Additionally, the interaction between PIH1 and H4K16 expels TIP5, a component of the silencing nucleolar remodeling complex (NoRC), from the core region, suggesting that PIH1 is involved in the derepression of NoRC-silenced rRNA genes. These data indicate that PIH1 is a positive regulator of human rRNA genes and is of great importance for the recovery of human cells from nutrient starvation and the transition to glucose-induced exponential growth in vivo.
authors.affiliation: Department of Biochemistry and Molecular Biology, National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, 5 Dongdan Santiao, Beijing 100005, China.
authors.forename: Niu
authors.initials: N
authors.lastname: Zhai
authors.forename: Zhong-liang
authors.initials: ZL
authors.lastname: Zhao
authors.forename: Mo-bin
authors.initials: MB
authors.lastname: Cheng
authors.forename: Yu-wei
authors.initials: YW
authors.lastname: Di
authors.forename: Hai-xia
authors.initials: HX
authors.lastname: Yan
authors.forename: Chun-yu
authors.initials: CY
authors.lastname: Cao
authors.forename: Hui
authors.initials: H
authors.lastname: Dai
authors.forename: Ye
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yu-fei
authors.initials: YF
authors.lastname: Shen
mesh_terms: D051017:Apoptosis Regulatory Proteins / Q000378:metabolism*; D042002:Chromatin Assembly and Disassembly / Q000187:drug effects; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D003850:Deoxyribonuclease I / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D020459:Genes, rRNA / Q000235:genetics; D005947:Glucose / Q000494:pharmacology*; D057809:HEK293 Cells; D006657:Histones / Q000378:metabolism*; D006801:Humans; D008954:Models, Biological; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding / Q000187:drug effects; D012322:RNA Precursors / Q000096:biosynthesis*; D000071796:SMARCB1 Protein; D014157:Transcription Factors
chemical_list: D051017:Apoptosis Regulatory Proteins; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D006657:Histones; C555143:PIH1D1 protein, human; D012322:RNA Precursors; D000071796:SMARCB1 Protein; C513266:SMARCB1 protein, human; D014157:Transcription Factors; D003850:Deoxyribonuclease I; D005947:Glucose