title: Outbreak of bullous impetigo caused by Staphylococcus aureus strains of phage type 3C/71 in a maternity ward linked to nasal carriage of a healthcare worker.
abstract: We describe an outbreak of bullous impetigo (BI) that occurred in a maternity unit and show phenotypic and genotypic properties and relatedness of isolated Staphylococcus aureus strains. Clinical material was obtained from 11 affected neonates. Additionally, nasal swabs from 67 healthy care workers (HCWs) as well as 107 environmental swabs were investigated. All isolates were screened for exfoliative toxin genes (eta, etb), antibiotic susceptibility and phage typed. Chromosomal DNA was genotyped by MLVF method and PCR/RFLP of coagulase gene were tested. Affected neonates were infected by two clusters of eta-positive S. aureus of phage type 3C/71: (1) MLVF type A isolates resistant only to penicillin, and (2) MLVF type B isolates resistant to penicillin and erythromycin/clindamycin. All isolates were susceptible to methicillin. We found 19 of 67 HCWs to be S. aureus nasal carriers. Two nasal isolates from HCWs were related to the outbreak on the basis of phage typing, PCR detection of eta/etb genes, antibiotyping and genotyping. Additionally, environmental swabs from the maternity unit revealed a 3C/71 S. aureus in the mattress of a baby bed. This is the first documented case of an outbreak of BI caused by phage type 3C/71 eta-positive strain of S. aureus.
authors.affiliation: Department of Medical Microbiology, Medical University of Gdańsk, Gdańsk, Poland.
authors.forename: Lidia
authors.initials: L
authors.lastname: Piechowicz
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Garbacz
authors.forename: Anna
authors.initials: A
authors.lastname: Budzyńska
authors.forename: Maria
authors.initials: M
authors.lastname: Dąbrowska-Szponar
mesh_terms: D000667:Ampicillin / Q000627:therapeutic use; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D015373:Bacterial Typing Techniques; D002353:Carrier State; D003428:Cross Infection / Q000188:drug therapy / Q000453:epidemiology* / Q000382:microbiology; D016172:DNA Fingerprinting; D004196:Disease Outbreaks; D024901:Drug Resistance, Multiple, Bacterial; D005838:Genotype; D006801:Humans; D007169:Impetigo / Q000188:drug therapy / Q000453:epidemiology* / Q000382:microbiology; D007231:Infant, Newborn; D017757:Infectious Disease Transmission, Professional-to-Patient; D008826:Microbial Sensitivity Tests; D058885:Multilocus Sequence Typing; D009775:Obstetrics and Gynecology Department, Hospital; D010564:Personnel, Hospital; D013211:Staphylococcus aureus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D013407:Sulbactam / Q000627:therapeutic use
chemical_list: D000900:Anti-Bacterial Agents; C035444:sultamicillin; D000667:Ampicillin; D013407:Sulbactam