title: Determination of Cu/Zn and Fe in human serum of patients with sickle cell anemia using radiation synchrotron.
abstract: In this work we analyzed serum samples from patients with Sickle Cell Anemia (SCA) using Total Reflection X-Ray Fluorescence using Synchrotron Radiation (SRTXRF). The SRTXRF measurements were performed at the X-Ray Fluorescence Beamline at the Brazilian National Synchrotron Light Laboratory (LNLS). We studied forty-three patients aged 18-50 suffering from SCA and sixty healthy volunteers aged 18-60. It was possible to determine the concentrations of the following elements: P, S, Cl, K, Ca, Fe, Cu, Zn, Br and Rb. Moreover, there are evidences of an association among Fe, Cu, Zn and Cu/Zn in the SCA pathogenesis process. The concentrations of Fe and Cu in the serum samples of patients with SCA were larger, 120% and 20%, respectively, when compared with the CG. The serum level Cu/Zn ratio was significantly higher (60%) in the serum samples from patients suffering from SCA than from the CG. Therefore, the Cu/Zn ratio can be used as an adjuvant index in enhancement for diagnosis of SCA.
authors.affiliation: Nuclear Instrumentation Laboratory, COPPE/UFRJ, P.O. Box 68509, 21.941-972 Rio de Janeiro, Brazil.
authors.forename: C G L
authors.initials: CG
authors.lastname: Canellas
authors.forename: S M F
authors.initials: SM
authors.lastname: Carvalho
authors.forename: M J
authors.initials: MJ
authors.lastname: Anjos
authors.forename: R T
authors.initials: RT
authors.lastname: Lopes
mesh_terms: D000755:Anemia, Sickle Cell / Q000097:blood*; D003300:Copper / Q000097:blood*; D006801:Humans; D007501:Iron / Q000097:blood*; D013052:Spectrometry, X-Ray Emission; D017356:Synchrotrons; D015032:Zinc / Q000097:blood*
chemical_list: D003300:Copper; D007501:Iron; D015032:Zinc