title: Analysis of association between TLR-4 Asp299Gly and Thr399Ile gene polymorphisms and chronic periodontitis in a sample of south Indian population.
abstract: BACKGROUND
  To analyze the association between TLR-4 Asp299Gly and Thr399Ile gene polymorphisms and chronic periodontitis in a sample of south Indian population.
  
  
  METHODS
  Genomic DNA was obtained from peripheral blood of 60 patients with chronic periodontitis and 60 periodontally healthy subjects. TLR-4 Asp299Gly and Thr399Ile gene polymorphisms were genotyped by a polymerase chain reaction-restriction fragment length polymorphism method. The data were analyzed by a χ(2)-test and by relative risk estimation.
  
  
  RESULTS
  Thr399Ile alleles were found in 4% of chronic periodontitis patients and in 1% of periodontally healthy subjects. The prevalence of a Thr399Ile heterozygote was found to be 5% in the chronic periodontitis group and 1.67% in the periodontally healthy group, respectively. Homozygosity for TLR-4 Thr399Ile was seen in chronic periodontitis patients only, which was 1.67%. The TLR-4 Asp299Gly gene polymorphism was not detected in either chronic periodontitis or periodontally healthy groups.
  
  
  CONCLUSIONS
  There is no significant association between TLR-4 Thr399Ile polymorphism and chronic periodontitis in a sample of south Indian population.
authors.affiliation: Department of Periodontics, SVS Institute of Dental Sciences, Appannapally, Mahabubnagar, Andhra Pradesh, India.
authors.forename: Bavigadda Harish
authors.initials: BH
authors.lastname: Reddy
authors.forename: N D
authors.initials: ND
authors.lastname: Jayakumar
authors.forename: Sreenivasa Rao
authors.initials: SR
authors.lastname: Akula
authors.forename: Rupali
authors.initials: R
authors.lastname: Sharma
authors.forename: G
authors.initials: G
authors.lastname: Kaarthikeyan
authors.lastname: Sankari