title: A method for the specific detection of resident bacteria in brine shrimp larvae.
abstract: In this study, we describe an easy but efficient method to specifically target the intestinal resident microbiota in brine shrimp larvae during DGGE analysis, hereby excluding the interference of both transient (luminal) bacteria and body surface bacteria. This effective technique has several advantages over alternative methods, with respect of ease of use and rapidity.
authors.affiliation: Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Rozier 44, 9000 Ghent, Belgium.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Niu
authors.forename: Tom
authors.initials: T
authors.lastname: Defoirdt
authors.forename: Anamaria
authors.initials: A
authors.lastname: Rekecki
authors.forename: Peter
authors.initials: P
authors.lastname: De Schryver
authors.forename: Wim
authors.initials: W
authors.lastname: Van den Broeck
authors.forename: Shuanglin
authors.initials: S
authors.lastname: Dong
authors.forename: Patrick
authors.initials: P
authors.lastname: Sorgeloos
authors.forename: Nico
authors.initials: N
authors.lastname: Boon
authors.forename: Peter
authors.initials: P
authors.lastname: Bossier
mesh_terms: D000818:Animals; D001156:Artemia / Q000382:microbiology*; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D016172:DNA Fingerprinting / Q000379:methods*; D058645:Denaturing Gradient Gel Electrophoresis / Q000379:methods*; D041981:Gastrointestinal Tract / Q000382:microbiology; D007814:Larva / Q000382:microbiology; D013997:Time Factors