title: MiR-3120 is a mirror microRNA that targets heat shock cognate protein 70 and auxilin messenger RNAs and regulates clathrin vesicle uncoating.
abstract: We show that a single gene locus gives rise to two fully processed and functional miRNAs, i.e. that due to imperfect base pairing, two distinct microRNAs (miRNAs) can be produced from the fully complementary DNA strands. The antisense strand encodes miR-214, which is transcribed by its own promoter, whereas a novel miRNA, miR-3120, is co-expressed with its host gene mRNA. We also found that miR-3120 regulates important aspects of cellular function that are similar to that of its host gene, dynamin-3. miR-3120 was found to be located in neuronal cell bodies and to target Hsc70 and auxilin, and its lentivirus-mediated expression inhibited the uncoating of clathrin-coated vesicles. Finally, mirror miRNAs are likely to represent a new group of miRNAs with complex roles in coordinating gene expression.
authors.affiliation: Henry Wellcome Laboratories for Integrated Neuroscience and Endocrinology, University of Bristol, Dorothy Hodgkin Building, Whitson Street, Bristol BS1 3NY, United Kingdom.
authors.forename: Helen
authors.initials: H
authors.lastname: Scott
authors.forename: Joanna
authors.initials: J
authors.lastname: Howarth
authors.forename: Youn Bok
authors.initials: YB
authors.lastname: Lee
authors.forename: Liang-Fong
authors.initials: LF
authors.lastname: Wong
authors.forename: Ioannis
authors.initials: I
authors.lastname: Bantounas
authors.forename: Leonidas
authors.initials: L
authors.lastname: Phylactou
authors.forename: Paul
authors.initials: P
authors.lastname: Verkade
authors.forename: James B
authors.initials: JB
authors.lastname: Uney
mesh_terms: D000818:Animals; D034021:Auxilins / Q000096:biosynthesis* / Q000235:genetics; D022163:Clathrin-Coated Vesicles / Q000235:genetics / Q000378:metabolism*; D034286:Dynamin III / Q000096:biosynthesis / Q000235:genetics; D005786:Gene Expression Regulation / Q000502:physiology; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar
chemical_list: D034021:Auxilins; D018840:HSP70 Heat-Shock Proteins; D035683:MicroRNAs; D012333:RNA, Messenger; D034286:Dynamin III