title: Identification of cancer cell-line origins using fluorescence image-based phenomic screening.
abstract: Universal phenotyping techniques that can discriminate among various states of biological systems have great potential. We applied 557 fluorescent library compounds to NCI's 60 human cancer cell-lines (NCI-60) to generate a systematic fluorescence phenotypic profiling data. By the kinetic fluorescence intensity analysis, we successfully discriminated the organ origin of all the 60 cell-lines.
authors.affiliation: Biomolecules Function Research Center, Korea Institute of Science and Technology, Seoul, South Korea.
authors.forename: Jun-Seok
authors.initials: JS
authors.lastname: Lee
authors.forename: Yun Kyung
authors.initials: YK
authors.lastname: Kim
authors.forename: Han Jo
authors.initials: HJ
authors.lastname: Kim
authors.forename: Siti
authors.initials: S
authors.lastname: Hajar
authors.forename: Yee Ling
authors.initials: YL
authors.lastname: Tan
authors.forename: Nam-Young
authors.initials: NY
authors.lastname: Kang
authors.forename: Shin Hui
authors.initials: SH
authors.lastname: Ng
authors.forename: Chang No
authors.initials: CN
authors.lastname: Yoon
authors.forename: Young-Tae
authors.initials: YT
authors.lastname: Chang
mesh_terms: D001896:Boron Compounds / Q000494:pharmacology; D045744:Cell Line, Tumor; D016000:Cluster Analysis; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007700:Kinetics; D008856:Microscopy, Fluorescence / Q000379:methods; D009930:Organic Chemicals / Q000494:pharmacology; D010641:Phenotype
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D005456:Fluorescent Dyes; D009930:Organic Chemicals; C107472:red dye CMXRos