title: Urinary steroid profile in early pregnancy after in vitro fertilization.
abstract: OBJECTIVE
  The aim of the study was to compare the levels of urinary steroid metabolites of patients with successful in vitro fertilization and patients who failed to achieve pregnancy.
  
  
  METHODS
  Comparison of urinary steroid profiles prior to oocyte pick-up and three weeks after embryo transfer.
  
  
  METHODS
  University hospital.
  
  
  METHODS
  Eleven women in the same age range with pregnancy after in vitro fertilization and eleven women who failed to achieve pregnancy.
  
  
  METHODS
  The standard "long" protocol was used for ovarian stimulation and intracytoplasmic sperm injection for assisted in vitro fertilization. The steroid metabolites in urine samples collected for 24 h were determined by gas chromatography-mass spectrometry.
  
  
  METHODS
  Steroid metabolite levels in urine samples determined in the early pregnancy period.
  
  
  RESULTS
  The levels of androsterone, etiocholanolone, pregnanediol, tetrahydro-11-dehydrocorticosterone and tetrahydro-corticosterone were significantly higher (p < 0.05) in the urine of women with successful pregnancy three weeks after the embryo transfer, while the levels of tetrahydrocortisone, tetrahydrocortisol, allo-tetrahydrocortisol and α-cortolone became higher in the group of patients with unsuccessful pregnancy.
  
  
  CONCLUSIONS
  The production of androgens, progesterone and corticoid steroid metabolites is altered in the early pregnancy period after in vitro fertilization.
authors.affiliation: Institute of Bioanalysis, Department of Obstetrics and Gynecology, Faculty of Medicine, University of Pécs, Édesanyák útja 17, Pécs, Hungary.
authors.forename: Ildikó
authors.initials: I
authors.lastname: Bíró
authors.forename: Anita
authors.initials: A
authors.lastname: Bufa
authors.forename: Ferenc
authors.initials: F
authors.lastname: Wilhelm
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Mánfai
authors.forename: Ferenc
authors.initials: F
authors.lastname: Kilár
authors.forename: Péter Miklós
authors.initials: PM
authors.lastname: Gocze
mesh_terms: D000328:Adult; D000738:Androsterone / Q000652:urine*; D003345:Corticosterone / Q000031:analogs & derivatives* / Q000652:urine; D005043:Etiocholanolone / Q000652:urine*; D005260:Female; D005307:Fertilization in Vitro; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D010062:Ovulation Induction; D011247:Pregnancy; D011256:Pregnancy Outcome; D011261:Pregnancy Trimester, First / Q000652:urine*; D011276:Pregnanediol / Q000652:urine*; D020554:Sperm Injections, Intracytoplasmic
chemical_list: C096314:tetrahydro-11-dehydrocorticosterone; C003676:tetrahydrocorticosterone; D005043:Etiocholanolone; D000738:Androsterone; D011276:Pregnanediol; D003345:Corticosterone