title: Reduced larger von Willebrand factor multimers at dawn in OSA plasmas reflect severity of apnoeic episodes.
abstract: Plasma von Willebrand factor (VWF), produced in and released from vascular endothelial cells by various stimuli including hypoxia, induces platelet aggregation under high shear stress and plays dual pivotal roles in haemostasis and thrombosis within arterioles, which are regulated by the size of vWF multimers (VWFMs). Patients with obstructive sleep apnoea (OSA) have increased risk of thrombotic cardiovascular events, but the pathogenesis is unclear. We examined the relationship between VWF and OSA by measuring VWF antigen (VWF:Ag), VWFMs, VWF collagen binding activity (VWF:CB) and a disintegrin-like, metalloproteinase, and thrombospiondin type 1 motifs 13. A total of 58 OSA patients were enrolled. Blood samples were collected before sleep, after sleep, and after one night of nasal continuous positive airway pressure therapy. Based on VWFM analysis, OSA patients were classified into three groups; consistently normal VWFMs (group 1, n=29), increased high molecular weight (HMW)-VWFMs at 06:00 h (group 2, n=18), and decreased or absent HMW-VWFMs at 06:00 h (group 3, n=11). Patients in group 3 had significantly worse apnoea/hypopnoea index; VWF:CB followed a similar pattern. We observed a significant decrease in platelet count between 21:00 h and 06:00 h in OSA patients, potentially associated with reduced larger VWFMs together with decreased VWF:Ag levels. Severe OSA may contribute to an arterial pro-thrombotic state.
authors.affiliation: Second Department of Internal Medicine, Nara Medical University, 840 Shijo-cho, Kashihara, Nara, 634-8522, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Koyama
authors.forename: Masanori
authors.initials: M
authors.lastname: Matsumoto
authors.forename: Shinji
authors.initials: S
authors.lastname: Tamaki
authors.forename: Masanori
authors.initials: M
authors.lastname: Yoshikawa
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Fujimura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kimura
mesh_terms: D051722:ADAM Proteins / Q000097:blood / Q000737:chemistry; D000071120:ADAMTS13 Protein; D000328:Adult; D003094:Collagen / Q000737:chemistry; D045422:Continuous Positive Airway Pressure; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010976:Platelet Count; D055503:Protein Multimerization; D012720:Severity of Illness Index; D020181:Sleep Apnea, Obstructive / Q000097:blood* / Q000628:therapy; D014841:von Willebrand Factor / Q000032:analysis / Q000737:chemistry*
chemical_list: D014841:von Willebrand Factor; D003094:Collagen; D051722:ADAM Proteins; D000071120:ADAMTS13 Protein; C099604:ADAMTS13 protein, human