title: Development of NIR fluorescent dyes based on Si-rhodamine for in vivo imaging.
abstract: We have developed a series of novel near-infrared (NIR) wavelength-excitable fluorescent dyes, SiR-NIRs, by modifying the Si-rhodamine scaffold to obtain emission in the range suitable for in vivo imaging. Among them, SiR680 and SiR700 showed sufficiently high quantum efficiency in aqueous media. Both antibody-bound and free dye exhibited high tolerance to photobleaching in aqueous solution. Subcutaneous xenograft tumors were successfully visualized in a mouse tumor model using SiR700-labeled anti-tenascin-C (TN-C) antibody, SiR700-RCB1. SiR-NIRs are expected to be useful as labeling agents for in vivo imaging studies including multicolor imaging, and also as scaffolds for NIR fluorescence probes.
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan.
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Koide
authors.forename: Yasuteru
authors.initials: Y
authors.lastname: Urano
authors.forename: Kenjiro
authors.initials: K
authors.lastname: Hanaoka
authors.forename: Wen
authors.initials: W
authors.lastname: Piao
authors.forename: Moriaki
authors.initials: M
authors.lastname: Kusakabe
authors.forename: Nae
authors.initials: N
authors.lastname: Saito
authors.forename: Takuya
authors.initials: T
authors.lastname: Terai
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Okabe
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Nagano
mesh_terms: D000818:Animals; D003952:Diagnostic Imaging / Q000379:methods*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry; D051379:Mice; D009374:Neoplasms, Experimental / Q000175:diagnosis*; D012235:Rhodamines / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D019265:Spectroscopy, Near-Infrared
chemical_list: D005456:Fluorescent Dyes; D012235:Rhodamines; D012822:Silicon Dioxide