title: Integrated pharmacokinetics of major bioactive components in MCAO rats after oral administration of Huang-Lian-Jie-Du-Tang.
abstract: BACKGROUND
  Huang-Lian-Jie-Du-Tang (HLJDT, or Oren-gedoku-to in Japanese), an important multi-herb remedy in China and other Asia countries, has been used clinically to treat cerebral ischemia for decades.
  
  
  METHODS
  According to the previous studies we have reported, an HPLC method was developed and validated for determination of berberine, palmatine, baicalin, baicalein and geniposide simultaneously in MCAO rat plasma after administration of HLJDT aqueous extract. A classified integral pharmacokinetic method was put forward after having compared the integrated concentration-time profile with that of single component. An AUC based weighting approach was used for integrated principle.
  
  
  RESULTS
  The results indicated the classified integral pharmacokinetic profile of index components from HLJDT could reveal the pharmacokinetic behavior of original components, and was corresponding to the holistic pharmacological effects of anti-ischemia with HLJDT.
  
  
  CONCLUSIONS
  This study was aimed to explore an approach that could be applied to integrate the pharmacokinetic behavior of different components derived from HLJDT. The integrated pharmacokinetic results also provided more information for further understanding of the clinical cerebrovascular disease in use of HLJDT.
authors.affiliation: Separation Engineering of Chinese Traditional Medicine Compound, Nanjing University of Chinese Medicine, Nanjing 210029, China.
authors.forename: Huaxu
authors.initials: H
authors.lastname: Zhu
authors.forename: Zhilei
authors.initials: Z
authors.lastname: Qian
authors.forename: Huan
authors.initials: H
authors.lastname: Li
authors.forename: Liwei
authors.initials: L
authors.lastname: Guo
authors.forename: Linmei
authors.initials: L
authors.lastname: Pan
authors.forename: Qichun
authors.initials: Q
authors.lastname: Zhang
authors.forename: Yuping
authors.initials: Y
authors.lastname: Tang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D019540:Area Under Curve; D001711:Biotransformation; D002317:Cardiovascular Agents / Q000008:administration & dosage* / Q000097:blood / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid; D004195:Disease Models, Animal; D004355:Drug Stability; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D020244:Infarction, Middle Cerebral Artery / Q000097:blood / Q000188:drug therapy*; D008297:Male; D008954:Models, Biological; D008517:Phytotherapy; D010946:Plants, Medicinal; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D002317:Cardiovascular Agents; D004365:Drugs, Chinese Herbal; C061676:oren gedoku to