title: Analysis of in vitro oxidized human LDL phospholipids by solid-phase extraction and micellar electrokinetic capillary chromatography.
abstract: Phospholipids of in vitro oxidized human low-density lipoproteins (LDL) were separated by two different solid-phase extraction (SPE) methods. One of the two methods was designed to test the effects of gradient elution. This SPE method isolated more phospholipids from in vitro oxidized LDL than the other one according to the results of liquid chromatography and electrospray ionization mass spectrometry (LC ESI-MS) analysis. A micellar electrokinetic capillary chromatography (MEKC) method was also used to analyze phospholipids separated by SPE. The results of MEKC and LC ESI-MS were consistent for the major phospholipid classes, including PC, lysoPC, PE, PI and PS. The MEKC profiles showed significant differences for native and oxidized LDL phospholipids. Therefore, the unique combination of SPE and MEKC methods showed dramatic distinctions between native and in vitro oxidized human LDL phospholipids. The combination also shows great potential for rapid analysis of in vivo oxidized human LDL phospholipids in the future.
authors.affiliation: Department of Chemistry, National Changhua University of Education, Changhua, Taiwan.
authors.forename: Ying-Ling
authors.initials: YL
authors.lastname: Yang
authors.forename: Chin-Pong
authors.initials: CP
authors.lastname: Chong
authors.forename: Ming-Hua
authors.initials: MH
authors.lastname: Tsai
authors.forename: Mine-Yine
authors.initials: MY
authors.lastname: Liu
mesh_terms: D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D006801:Humans; D008077:Lipoproteins, LDL / Q000737:chemistry* / Q000302:isolation & purification; D010743:Phospholipids / Q000737:chemistry* / Q000302:isolation & purification; D052616:Solid Phase Extraction / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
chemical_list: D008077:Lipoproteins, LDL; D010743:Phospholipids; C099252:oxidized low density lipoprotein