title: Synthesis of [12]aneN3-dipeptide conjugates as metal-free DNA nucleases.
abstract: In this Letter, a series of macrocyclic polyamine [12]aneN(3)-dipeptide conjugates as a new type of metal-free nucleases were synthesized and fully characterized with (1)H NMR, (13)C NMR, IR, and HR-MS. Results indicate that these conjugates can bind to calf thymus DNA mainly through electrostatic interaction and can cleave the plasmid DNA at 200 μM (pH 7.2, 37°C), with an acceleration of 10(6)-fold via hydrolytic pathway.
authors.affiliation: College of Chemistry, Beijing Normal University, Beijing, China.
authors.forename: Zhi-Fen
authors.initials: ZF
authors.lastname: Li
authors.forename: Hua-Long
authors.initials: HL
authors.lastname: Chen
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Zhang
authors.forename: Zhong-Lin
authors.initials: ZL
authors.lastname: Lu
mesh_terms: D000818:Animals; D002417:Cattle; D004247:DNA; D053837:DNA Cleavage; D003851:Deoxyribonucleases / Q000737:chemistry; D004151:Dipeptides / Q000737:chemistry*; D004587:Electrophoresis, Agar Gel; D006868:Hydrolysis; D047028:Macrocyclic Compounds / Q000138:chemical synthesis*; D009682:Magnetic Resonance Spectroscopy; D018716:Molecular Mimicry; D011073:Polyamines / Q000737:chemistry*
chemical_list: D004151:Dipeptides; D047028:Macrocyclic Compounds; D011073:Polyamines; D004247:DNA; C427203:calf thymus DNA; D003851:Deoxyribonucleases