title: Diagnostic utility of notch-1 immunocytochemistry in endometrial cytology.
abstract: OBJECTIVE
  It was the aim of this study to evaluate the diagnostic utility of Notch-1 immunocytochemistry in distinguishing endometrial glandular and stromal breakdown (EGBD) from endometrial adenocarcinoma in endometrial cytology.
  
  
  METHODS
  Samples of normal endometrium, EGBD and endometrial adenocarcinoma were subjected to immunocytochemical staining for Notch-1, and we examined the labeling index (LI) of Notch-1 (the ratio of intranuclear Notch-1-positive cells to total cells). We compared (1) the Notch-1 LI in normal endometrium, (2) the Notch-1 LI between normal endometrium and endometrial adenocarcinoma, and (3) the Notch-1 LI in normal endometrium, EGBD and endometrial adenocarcinoma.
  
  
  RESULTS
  In analysis item 1, the LI of Notch-1 was 32.9 ± 8.4, 19.4 ± 8.2 and 12.5 ± 7.5% in proliferative endometrium, secretory endometrium and atrophic endometrium, respectively. In analysis item 2, the LI of Notch-1 in endometrial adenocarcinoma was 45.2 ± 7.4%, which was significantly higher than that in normal endometrium. In analysis item 3, the LI of Notch-1 in EGBD was 31.3 ± 8.3%, which was significantly lower than that in endometrial adenocarcinoma.
  
  
  CONCLUSIONS
  In conclusion, Notch-1 immunocytochemistry is a useful method for distinguishing between EGBD and endometrial carcinoma in endometrial cytology.
authors.affiliation: Department of Chemical Technology, Graduate School of Science and Industrial Technology, Kurashiki, Japan. m-mori@sci.kusa.ac.jp
authors.forename: Michihiro
authors.initials: M
authors.lastname: Mori
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Miyamoto
authors.forename: Setsuyo
authors.initials: S
authors.lastname: Ohno
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Miyake
authors.forename: Takuya
authors.initials: T
authors.lastname: Sakaguchi
authors.forename: Eiji
authors.initials: E
authors.lastname: Ohno
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis; D001706:Biopsy / Q000379:methods; D002452:Cell Count; D016889:Endometrial Neoplasms / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D004717:Endometrium / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008875:Middle Aged; D051881:Receptor, Notch1 / Q000032:analysis*; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; C497761:NOTCH1 protein, human; D051881:Receptor, Notch1