title: Type-specific human papillomavirus DNA detected in atypical glandular cell Pap tests.
abstract: OBJECTIVE
  To evaluate the presence of type-specific human papillomavirus (HPV) DNA in atypical glandular cells (AGCs) from cervical liquid-based cytology and to report the relationship between HPV types and cervical histological abnormalities.
  
  
  METHODS
  We used a nested multiplex polymerase chain reaction assay to test AGC Papanicolaou (Pap) tests for the presence of 14 high-risk (HR) HPV types.
  
  
  RESULTS
  HR HPV types were detected in 33 of 161 AGC Pap tests (20.3%). Types 16 and/or 18 were detected in 13 samples (8%). Eight other HPV types were detected in 1-4 samples each. HPV-associated disease was diagnosed in 8 AGC cases (8%) with available histology results. The sensitivity and specificity of the HR HPV test were 87.5 and 90%, respectively, and the negative predictive value (NPV) was 99%. For a test that can isolate HPV types 16 and 18, the sensitivity and specificity were 62.5 and 100%, respectively, the positive predictive value (PPV) was 100% and the NPV was 97%.
  
  
  CONCLUSIONS
  HPV 16 and 18 were the most common types detected in AGC Paps. We found high specificity, PPV and NPV with a test that can isolate these 2 HPV types. These results indicate a possible role for type-specific HPV testing in the management of AGC Pap tests.
authors.affiliation: Department of Family Medicine and Department of Anatomic Pathology, Oakland University William Beaumont School of Medicine, Rochester, MI 48314, USA. emulhem@beaumont.edu
authors.forename: Elie
authors.initials: E
authors.lastname: Mulhem
authors.forename: Mitual
authors.initials: M
authors.lastname: Amin
authors.forename: Jonathan
authors.initials: J
authors.lastname: Copeland
authors.forename: John
authors.initials: J
authors.lastname: Sharma
authors.forename: Sue
authors.initials: S
authors.lastname: Hunter
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002277:Carcinoma / Q000235:genetics / Q000473:pathology / Q000821:virology; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D052162:Human papillomavirus 16 / Q000235:genetics / Q000302:isolation & purification*; D052161:Human papillomavirus 18 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008875:Middle Aged; D065006:Papanicolaou Test; D030361:Papillomavirus Infections / Q000235:genetics / Q000473:pathology* / Q000821:virology; D012189:Retrospective Studies; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000473:pathology* / Q000821:virology; D014626:Vaginal Smears / Q000379:methods*; D055815:Young Adult
chemical_list: D015344:DNA Probes, HPV; D004279:DNA, Viral