title: The last step of kanamycin biosynthesis: unique deamination reaction catalyzed by the α-ketoglutarate-dependent nonheme iron dioxygenase KanJ and the NADPH-dependent reductase KanK.
abstract: Mystery solved: using heterologous expression, the activities of two enzymes exclusively belonging to the kanamycin biosynthetic pathway have been identified in vitro. A distinctive reaction mechanism to produce kanamycin is proposed and the previously unknown catalytic deamination activity of KanJ dioxygenase is uncovered.
authors.affiliation: Department of Chemistry and Materials Science, Tokyo Institute of Technology, O-okayama, Meguro-ku, Japan.
authors.forename: Hilda
authors.initials: H
authors.lastname: Sucipto
authors.forename: Fumitaka
authors.initials: F
authors.lastname: Kudo
authors.forename: Tadashi
authors.initials: T
authors.lastname: Eguchi
mesh_terms: D000588:Amines / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000737:chemistry; D001426:Bacterial Proteins / Q000378:metabolism*; D002384:Catalysis; D003641:Deamination; D049308:Dioxygenases / Q000378:metabolism*; D007612:Kanamycin / Q000096:biosynthesis* / Q000737:chemistry; D005810:Multigene Family; D010088:Oxidoreductases / Q000378:metabolism*; D013302:Streptomyces / Q000201:enzymology
chemical_list: D000588:Amines; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D007612:Kanamycin; D010088:Oxidoreductases; D049308:Dioxygenases