title: [Effect of tetrandrine on bax, bcl-2 and TGF-β2 mRNA expressions in cultured human Tenon's capsule fibroblasts].
abstract: OBJECTIVE
  To investigate the effect of tetrandrine (Tet) on the expression of bax, bcl-2, and transforming growth factor-β2 (TGF-β2) mRNA in cultured human fibroblasts of Tenon's capsules (TCFS) and explore its possible mechanism.
  
  
  METHODS
  The third passage of TCFS cultured in vitro were exposed to 1×10(-5) mol/L Tet for 24 h, and real-time fluorescence quantitative PCR was used to detect the changes in the expressions of bax, bcl-2, and TGF-β2 mRNA.
  
  
  RESULTS
  The expression level of bax mRNA was obviously higher, while bcl-2 and TGF-β2 mRNA levels were significantly lower in Tet-treated TCFS than those in the control cells (P<0.05).
  
  
  CONCLUSIONS
  Tet can  inhibit  the proliferation of TCFS possibly by reducing the expressions of bcl-2 and TGF-β2 mRNA, enhancing the expression of bax mRNA and inducing cell apoptosis, suggesting its potential in preventing fibrous scar formation after glaucoma filtration surgery.
authors.affiliation: Institute of Ophthalmology, Xianning College in Hubei Province, Xianning, China. lidai008@21cn. com
authors.forename: Dai
authors.initials: D
authors.lastname: Li
authors.forename: Ming
authors.initials: M
authors.lastname: Sun
authors.forename: Qingchun
authors.initials: Q
authors.lastname: Li
authors.forename: Fang
authors.initials: F
authors.lastname: Yang
authors.forename: Shaochun
authors.initials: S
authors.lastname: Lin
authors.forename: Kaili
authors.initials: K
authors.lastname: Wu
mesh_terms: D017209:Apoptosis; D044182:Benzylisoquinolines / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002921:Cicatrix / Q000378:metabolism / Q000517:prevention & control; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D058475:Tenon Capsule / Q000166:cytology*; D053781:Transforming Growth Factor beta2 / Q000235:genetics / Q000378:metabolism*; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism*
chemical_list: C494826:BAX protein, human; D044182:Benzylisoquinolines; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; C509041:TGFB2 protein, human; D053781:Transforming Growth Factor beta2; D051028:bcl-2-Associated X Protein; C009438:tetrandrine