title: [DNA extraction from decomposed tissue by double-digest and magnetic beads methods].
abstract: OBJECTIVE
  To study the effect of the double-digest and magnetic beads method for DNA extraction from 3 types of decomposed tissues.
  
  
  METHODS
  DNA of cartilages, nails and joint capsule in 91 highly decomposed corpses which had not been extracted by common magnetic beads method, were prepared with the double-digest and magnetic beads methods, and quantified with Quantifiler kit, followed by amplification with Sinofiler kit or Minifiler kit.
  
  
  RESULTS
  DNA concentration extracted from the 91 highly decomposed cartilages, nails and joint capsule samples was 0-0.225 ng/microL. Sixty-two samples whose DNA concentration were more than 0.020 ng/microL had obtained 9 or more STR loci successfully. The detection rate was 68.13%.
  
  
  CONCLUSIONS
  The successful rate of STR genotyping for the 3 types of decomposed tissues can be significantly improved by the double-digest and magnetic beads methods.
authors.affiliation: Guangzhou Institute of Criminal Science and Technology, Guangzhou 510030, China.
authors.forename: Dian
authors.initials: D
authors.lastname: Yang
authors.forename: Chao
authors.initials: C
authors.lastname: Liu
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
mesh_terms: D002102:Cadaver; D002356:Cartilage; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D016172:DNA Fingerprinting / Q000379:methods*; D005554:Forensic Medicine / Q000379:methods*; D006801:Humans; D017746:Joint Capsule; D008280:Magnetics; D009262:Nails; D016133:Polymerase Chain Reaction; D011933:Reagent Kits, Diagnostic; D012117:Resins, Synthetic; D020080:Tandem Repeat Sequences
chemical_list: D011933:Reagent Kits, Diagnostic; D012117:Resins, Synthetic; D004247:DNA