title: The promoter region of the Zinnia elegans basic peroxidase isoenzyme gene contains cis-elements responsive to nitric oxide and hydrogen peroxide.
abstract: NO and H2O2 are important biological messengers in plants. They are formed during xylem differentiation in Zinnia elegans and apparently play important roles during the xylogenesis. To ascertain the responsiveness of the Z. elegans peroxidase (ZePrx) to these endogenous signals, the effects of NO and H2O2 on ZePrx were studied. The results showed that ZePrx is up-regulated by NO and H2O2, as confirmed by RT-qPCR, and that its promoter contains multiple copies of all the putative cis-elements (ACGT box, OCS box, OPAQ box, L1BX, MYCL box and W box) known to confer regulation by NO and H2O2. Like other OCS elements, the OCS element of ZePrx contains the sequence TACG that is recognized by OBF5, a highly conserved bZIP transcription factor, and the 10 bp sequence, ACAaTTTTGG, which is recognized by OBP1, a Dof domain protein that binds down-stream the OCS element. Furthermore, the ZePrx OCS element is flanked by two CCAAT-like boxes, and encloses one auxin-responsive ARFAT element and two GA3-responsive Pyr boxes. Results also showed that ZePrx may be described as the first protein to be up-regulated by NO and H2O2, whose mRNA contains several short-longevity conferring elements, such as a downstream (DST) sequence analogous to the DSTs contained in the highly unstable SAUR transcripts. The presence of these regulatory elements strongly suggests that ZePrx is finely regulated, as one may expect from an enzyme that catalyzes the last irreversible step of the formation of lignins, the major irreversible sink for the photosynthetically fixed CO2.
authors.affiliation: Department of Plant Biology, University of Murcia, 30100, Murcia, Spain.
authors.forename: Laura V
authors.initials: LV
authors.lastname: Gómez-Ros
authors.forename: Carlos
authors.initials: C
authors.lastname: Gabaldón
authors.forename: María José
authors.initials: MJ
authors.lastname: López Núñez-Flores
authors.forename: Jorge
authors.initials: J
authors.lastname: Gutiérrez
authors.forename: Joaquín
authors.initials: J
authors.lastname: Herrero
authors.forename: José Miguel
authors.initials: JM
authors.lastname: Zapata
authors.forename: Mariana
authors.initials: M
authors.lastname: Sottomayor
authors.forename: Juan
authors.initials: J
authors.lastname: Cuello
authors.forename: Alfonso
authors.initials: A
authors.lastname: Ros Barceló
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics; D019659:Asteraceae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007527:Isoenzymes / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D008031:Lignin / Q000032:analysis; D008969:Molecular Sequence Data; D009569:Nitric Oxide / Q000494:pharmacology*; D059372:Nucleotide Motifs; D009195:Peroxidase / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics*; D018749:RNA, Plant / Q000235:genetics; D020218:Response Elements / Q000235:genetics; D036226:Seedlings / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D016415:Sequence Alignment; D015854:Up-Regulation
chemical_list: D020121:5' Untranslated Regions; D018076:DNA, Complementary; D018744:DNA, Plant; D007527:Isoenzymes; D018749:RNA, Plant; D009569:Nitric Oxide; D008031:Lignin; D006861:Hydrogen Peroxide; D009195:Peroxidase