title: Active regulation of the epidermal calcium profile.
abstract: A distinct calcium profile is strongly implicated in regulating the multi-layered structure of the epidermis. However, the mechanisms that govern the regulation of this calcium profile are currently unclear. It clearly depends on the relatively impermeable barrier of the stratum corneum (passive regulation) but may also depend on calcium exchanges between keratinocytes and extracellular fluid (active regulation). Using a mathematical model that treats the viable sublayers of unwounded human and murine epidermis as porous media and assumes that their calcium profiles are passively regulated, we demonstrate that these profiles are also actively regulated. To obtain this result, we found that diffusion governs extracellular calcium motion in the viable epidermis and hence intracellular calcium is the main source of the epidermal calcium profile. Then, by comparison with experimental calcium profiles and combination with a hypothesised cell velocity distribution in the viable epidermis, we found that the net influx of calcium ions into keratinocytes from extracellular fluid may be constant and positive throughout the stratum basale and stratum spinosum, and that there is a net outflux of these ions in the stratum granulosum. Hence, the calcium exchange between keratinocytes and extracellular fluid differs distinctly between the stratum granulosum and the underlying sublayers, and these differences actively regulate the epidermal calcium profile. Our results also indicate that plasma membrane dysfunction may be an early event during keratinocyte disintegration in the stratum granulosum.
authors.affiliation: Discipline of Mathematical Sciences, Faculty of Science and Technology, Queensland University of Technology, GPO Box 2434, Brisbane 4001, Australia. mp.adams@qut.edu.au
authors.forename: M P
authors.initials: MP
authors.lastname: Adams
authors.forename: D G
authors.initials: DG
authors.lastname: Mallet
authors.forename: G J
authors.initials: GJ
authors.lastname: Pettet
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D049109:Cell Proliferation; D000078404:Epidermal Cells; D004817:Epidermis / Q000033:anatomy & histology / Q000378:metabolism*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism; D051379:Mice; D008954:Models, Biological
chemical_list: D002118:Calcium