title: Multiplex PCR panel of microsatellite markers for the  tambaqui, Colossoma macropomum, developed as a tool for use in conservation and  broodstock management.
abstract: The  tambaqui, Colossoma macropomum, native to Brazil, is widely used in  aquaculture systems. We developed a multiplex PCR panel for this species,  comprising 12 microsatellite loci. This panel was used to genotype 73 specimens  collected from Juruti, a city in the Brazilian Amazon. The mean number of  alleles per locus was 8.8, the mean observed heterozygosity was 0.76, and the  combined power of discrimination and the combined power of exclusion were  0.99999999999999993 and 0.999991762, respectively. We observed no significant  deviation from Hardy-Weinberg equilibrium in this population. All amplified  alleles were clearly typed, and easily interpretable results were obtained.  This method will be useful for paternity analysis, population genetics and  conservation studies, as well as for selective breeding programs for C.  macropomum.
authors.affiliation: Laboratório de Genética Humana e Médica, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brasil. ighamoy@gmail.com
authors.forename: I G
authors.initials: IG
authors.lastname: Hamoy
authors.forename: S
authors.initials: S
authors.lastname: Santos
mesh_terms: D000483:Alleles; D000818:Animals; D017756:Aquaculture; D060126:Characidae / Q000235:genetics*; D005787:Gene Frequency; D014644:Genetic Variation; D005828:Genetics, Population; D005838:Genotype; D018895:Microsatellite Repeats / Q000235:genetics*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*