title: Effects of laser polar-body biopsy on embryo quality.
abstract: OBJECTIVE
  To evaluate the effect of laser polar-body biopsy (PBB) for preimplantation genetic diagnosis on embryo quality.
  
  
  METHODS
  Retrospective case-control analysis. The quality of 145 embryos after PBB was compared to 276 embryos of the same group of women without biopsy.
  
  
  METHODS
  University-based tertiary-care medical center.
  
  
  METHODS
  Women with inherited genetics disease.
  
  
  METHODS
  Laser PBB of IVF embryos for genetic diagnosis.
  
  
  METHODS
  The study and control embryos were compared for fertilization rate, pronuclear grading, and cleavage-stage parameters on days 1, 2, and 3 after oocyte retrieval.
  
  
  RESULTS
  The study embryos demonstrated higher rates of cleavage arrest (3.6% vs. 0.7%), higher rate of significant fragmentation on day 2 (9.5% vs. 3.0%), and lower rate of good cleavage embryos on day 2 (69.1% vs. 78.4%) compared with control embryos. On day 3, the study embryos had lower cleavage rates (six or more blastomeres; 56.5% vs. 74.5%), higher fragmentation (11.7% vs. 3.9%), higher rate of embryos presenting inferior cleavage pattern (57.2% vs. 38.5%), and lower mean blastomere number (5.8 ± 2.1 vs. 6.6 ± 1.9) compared with control embryos.
  
  
  CONCLUSIONS
  Polar-body biopsy may have a negative effect on embryo quality.
authors.affiliation: Sarah Racine IVF Unit, Lis Maternity Hospital, Tel Aviv Sourasky Medical Center, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel. ishai.levin@gmail.com
authors.forename: Ishai
authors.initials: I
authors.lastname: Levin
authors.forename: Benny
authors.initials: B
authors.lastname: Almog
authors.forename: Tamar
authors.initials: T
authors.lastname: Shwartz
authors.forename: Veronica
authors.initials: V
authors.lastname: Gold
authors.forename: Dalit
authors.initials: D
authors.lastname: Ben-Yosef
authors.forename: Michal
authors.initials: M
authors.lastname: Shaubi
authors.forename: Ami
authors.initials: A
authors.lastname: Amit
authors.forename: Mira
authors.initials: M
authors.lastname: Malcov
mesh_terms: D000328:Adult; D001706:Biopsy / Q000009:adverse effects / Q000295:instrumentation / Q000379:methods; D001755:Blastocyst / Q000473:pathology*; D016009:Chi-Square Distribution; D002970:Cleavage Stage, Ovum; D053938:DNA Fragmentation; D005260:Female; D005307:Fertilization in Vitro; D030342:Genetic Diseases, Inborn / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D006801:Humans; D007557:Israel; D007834:Lasers / Q000009:adverse effects; D016015:Logistic Models; D015999:Multivariate Analysis; D054315:Oocyte Retrieval; D059705:Polar Bodies / Q000473:pathology*; D011237:Predictive Value of Tests; D019836:Preimplantation Diagnosis / Q000009:adverse effects / Q000295:instrumentation / Q000379:methods; D012189:Retrospective Studies