title: Genetic variation of Fusarium oxysporum isolates forming fumonisin B(1) and moniliformin.
abstract: Thirty single-spore isolates of a toxigenic fungus, Fusarium oxysporum, were isolated from asparagus spears and identified by species-specific polymerase chain reaction (PCR) and translation elongation factor 1-α (TEF) sequence analysis. In the examined sets of F. oxysporum isolates, the DNA sequences of mating type genes (MAT) were identified. The distribution of MAT idiomorph may suggest that MAT1-2 is a predominant mating type in the F. oxysporum population. F. oxysporum is mainly recognised as a producer of moniliformin-the highly toxic secondary metabolite. Moniliformin content was determined by high-performance liquid chromatography (HPLC) analysis in the range 0.05-1,007.47 μg g(-1) (mean 115.93 μg g(-1)) but, also, fumonisin B(1) was detected, in the concentration range 0.01-0.91 μg g(-1) (mean 0.19 μg g(-1)). There was no association between mating types and the mycotoxins biosynthesis level. Additionally, a significant intra-species genetic diversity was revealed and molecular markers associated with toxins biosynthesis were identified.
authors.affiliation: Department of Phytopathology, Poznan University of Life Sciences, Dąbrowskiego 159, 60-594 Poznań, Polan. irzyk@up.poznan.pl
authors.forename: Lidia
authors.initials: L
authors.lastname: Irzykowska
authors.forename: Jan
authors.initials: J
authors.lastname: Bocianowski
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Waśkiewicz
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Weber
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Karolewski
authors.forename: Piotr
authors.initials: P
authors.lastname: Goliński
authors.forename: Marian
authors.initials: M
authors.lastname: Kostecki
authors.forename: Witold
authors.initials: W
authors.lastname: Irzykowski
mesh_terms: D027761:Asparagus Plant / Q000382:microbiology; D015415:Biomarkers / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003503:Cyclobutanes / Q000378:metabolism*; D016172:DNA Fingerprinting; D037341:Fumonisins / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D005670:Fusarium / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D049770:Genes, Mating Type, Fungal; D014644:Genetic Variation; D020648:Peptide Elongation Factor 1 / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013172:Spores, Fungal / Q000502:physiology
chemical_list: D015415:Biomarkers; D003503:Cyclobutanes; D037341:Fumonisins; D005656:Fungal Proteins; D020648:Peptide Elongation Factor 1; C008539:moniliformin; C056933:fumonisin B1