title: Metadherin regulates radioresistance in cervical cancer cells.
abstract: Metadherin (MTDH) promotes cancer metastasis, chemoresistance, invasion             and angiogenesis. Upregulation of MTDH is correlated with both progression and             poor clinical outcome of many types of cancers; however, there is currently no             information regarding the role of MTDH in radiation sensitivity. Here, we investigated             the effects of MTDH on the radiosensitivity of cervical cancer cells using the             SiHa cell line. We discovered that cervical cancer cells in which MTDH was knocked             down had significantly increased radiosensitivity as measured by a clonogenic             assay. MTDH knockdown cells also had increased apoptosis and a decreased proportion             of cells arrested in the G2 phase after radiation treatment. MTDH knockdown also             weakened the repair of DNA double-strand breaks (DSBs) induced by radiation. These             results indicate that MTDH affects the radiosensitivity of cervical cancer cells             and that MTDH may be a novel target to improve cervical cancer radiation response.
authors.affiliation: Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Ji'nan, Shandong 250012, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhao
authors.forename: Meena S
authors.initials: MS
authors.lastname: Moran
authors.forename: Qifeng
authors.initials: Q
authors.lastname: Yang
authors.forename: Qiao
authors.initials: Q
authors.lastname: Liu
authors.forename: Cunzhong
authors.initials: C
authors.lastname: Yuan
authors.forename: Shuhui
authors.initials: S
authors.lastname: Hong
authors.forename: Beihua
authors.initials: B
authors.lastname: Kong
mesh_terms: D017209:Apoptosis / Q000235:genetics / Q000528:radiation effects; D015815:Cell Adhesion Molecules / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D053903:DNA Breaks, Double-Stranded / Q000528:radiation effects; D005260:Female; D059565:G2 Phase Cell Cycle Checkpoints / Q000235:genetics / Q000528:radiation effects; D020868:Gene Silencing; D006801:Humans; D008565:Membrane Proteins; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D016601:RNA-Binding Proteins; D011836:Radiation Tolerance / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000378:metabolism
chemical_list: D015815:Cell Adhesion Molecules; C483039:MTDH protein, human; D008565:Membrane Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins