title: Initial proteome analysis of caffeine-induced proteins in Aspergillus tamarii using two-dimensional fluorescence difference gel electrophoresis.
abstract: Caffeine is toxic to most microorganisms. However, some filamentous fungi, such as Aspergillus tamarii, are able to metabolize this alkaloid when fed caffeine as the sole nitrogen source. The aim of the present work was to identify intracellular A. tamarii proteins, regulated by caffeine, using fluorescence difference two-dimensional gel electrophoresis. Specific proteins from two culture media of A. tamarii grown either on ammonium sulfate or caffeine as the sole nitrogen source were analysed by mass spectrometry. Thirteen out of a total of 85 differentially expressed spots were identified after database search. Identified up-regulated proteins include phosphoglycerate kinase, malate dehydrogenase, dyp-type peroxidase family protein, heat shock protein, Cu, Zn superoxidase dismutase and xanthine dehydrogenase. Some of the proteins identified in this study are involved in the caffeine degradation pathway as well as in stress response, suggesting that stress proteins could be involved in caffeine metabolism in filamentous fungi.
authors.affiliation: Institut de Recherche pour le Développement, Faculté des Sciences de Saint Jérôme; Case 441, Université Paul Cézanne, Marseille, France. gerardo@ccrc.uga.edu
authors.forename: Gerardo
authors.initials: G
authors.lastname: Gutiérrez-Sánchez
authors.forename: James
authors.initials: J
authors.lastname: Atwood
authors.forename: V S Kumar
authors.initials: VS
authors.lastname: Kolli
authors.forename: Sévastianos
authors.initials: S
authors.lastname: Roussos
authors.forename: Christopher
authors.initials: C
authors.lastname: Augur
mesh_terms: D001230:Aspergillus / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D002110:Caffeine / Q000494:pharmacology*; D015536:Down-Regulation / Q000187:drug effects; D005656:Fungal Proteins / Q000302:isolation & purification / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D013058:Mass Spectrometry; D040901:Proteomics / Q000379:methods*; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D013050:Spectrometry, Fluorescence; D057825:Two-Dimensional Difference Gel Electrophoresis / Q000379:methods*; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D005656:Fungal Proteins; D000644:Quaternary Ammonium Compounds; D002110:Caffeine