title: MMP mediated type V collagen degradation (C5M) is elevated in ankylosing spondylitis.
abstract: OBJECTIVE
  Type V collagen has been demonstrated to control fibril formation. The aim of this study was to develop an ELISA capable of detecting a fragment of type V collagen generated by MMP-2/9 and to evaluate the assay as biomarker for ankylosing spondylitis (AS).
  
  
  METHODS
  A fragment unique to type V collagen and generated by both MMP-2/9 cleaved at the amino acid position 1317 (C5M) was selected for ELISA development. 40 AS patients and 40 age-matched controls were evaluated.
  
  
  RESULTS
  An ELISA detecting C5M with inter- and intra-assay variations of 9.1% and 4.4% was developed. C5M levels were significantly higher in AS patients compared to controls, 229% (p<0.0001). The diagnostic AUC was 83%.
  
  
  CONCLUSIONS
  This ELISA is the first for detecting type V collagen degradation. AS patients had highly elevated levels of MMP mediated type V collagen degradation. The prognostic and diagnostic values need to be further investigated in additional clinical settings.
authors.affiliation: Nordic Bioscience A/S, Herlev, Denmark. ssv@nordicbioscience.com
authors.forename: S S
authors.initials: SS
authors.lastname: Veidal
authors.forename: D V
authors.initials: DV
authors.lastname: Larsen
authors.forename: X
authors.initials: X
authors.lastname: Chen
authors.forename: S
authors.initials: S
authors.lastname: Sun
authors.forename: Q
authors.initials: Q
authors.lastname: Zheng
authors.forename: A-C
authors.initials: AC
authors.lastname: Bay-Jensen
authors.forename: D J
authors.initials: DJ
authors.lastname: Leeming
authors.forename: A
authors.initials: A
authors.lastname: Nawrocki
authors.forename: M R
authors.initials: MR
authors.lastname: Larsen
authors.forename: G
authors.initials: G
authors.lastname: Schett
authors.forename: M A
authors.initials: MA
authors.lastname: Karsdal
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D016022:Case-Control Studies; D024142:Collagen Type VI / Q000097:blood* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D010446:Peptide Fragments; D011379:Prognosis; D059748:Proteolysis; D012189:Retrospective Studies; D013167:Spondylitis, Ankylosing / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology*; D055815:Young Adult
chemical_list: D000911:Antibodies, Monoclonal; D015415:Biomarkers; D024142:Collagen Type VI; D010446:Peptide Fragments; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9