title: Simple and rapid protocol for  the isolation of PCR-amplifiable DNA from medicinal plants.
abstract: Medicinal  plant species has a valuable economic importance because of its usage as  pharmaceuticals, nutritional, as well as its use in popular medication. For  DNA-based techniques, nanogram quantities of the purified DNA are requisite to  amplify and yield sufficient amounts of PCR products. SDS-based DNA isolation  method was used to extract DNA from 11 species of different aromatic and  medicinal plants collected from Saudi Arabia. Three hundred milligrams of fresh  shredded plant material was necessary. The DNA purity was further confirmed by  agarose gel, restriction endonuclease digestion and microsatellite  primed-polymerase chain reaction (MP-PCR). DNA yields ranged from 10-20 μg (in 100-μL elution volumes) from all plant  material evaluated. The DNA obtained was free of any contaminating proteins,  polysaccharides and colored pigments. The extracted genomic DNA was found  suitable for restriction digestion and PCR amplification. Our experimental  procedure provides an easy, suitable, non-toxic, cheap, and quick process for  the amplification of DNA from medical plant tissue.
authors.affiliation: Botany and Microbiology Department, College of Science, King Saud University, Riyadh, Saudi Arabia.
authors.forename: A A
authors.initials: AA
authors.lastname: Alatar
authors.forename: M A
authors.initials: MA
authors.lastname: Mahmoud
authors.forename: S A
authors.initials: SA
authors.lastname: Al-Sohaibani
authors.forename: K A
authors.initials: KA
authors.lastname: Abd-Elsalam
mesh_terms: D019306:Artemisia / Q000235:genetics; D029773:Citrullus / Q000235:genetics; D018744:DNA, Plant / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification*; D023281:Genomics; D018895:Microsatellite Repeats; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D010946:Plants, Medicinal / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods
chemical_list: D018744:DNA, Plant