title: Conversion of spent mushroom substrate to biofertilizer using a stress-tolerant phosphate-solubilizing Pichia farinose FL7.
abstract: To develop high-efficient biofertilizer, an environmental stress-tolerant phosphate-solubilizing microorganism (PSM) was isolated from agricultural wastes compost, and then applied to spent mushroom substrate (SMS). The isolate FL7 was identified as Pichia farinose with resistance against multiple environmental stresses, including 5-45°C temperature, 3-10 pH range, 0-23% (w/v) NaCl and 0-6M ammonium ion. Under the optimized cultivation condition, 852.8 mg/l total organic acids can be produced and pH can be reduced to 3.8 after 60 h, meanwhile, the soluble phosphate content reached 816.16 mg/l. The P. farinose was used to convert SMS to a phosphate biofertilizer through a semi-solid fermentation (SSF) process. After fermentation of 10 days, cell density can be increased to 5.6 × 10(8)CFU/g in biomass and pH in this medium can be decreased to 4.0. SMS biofertilizer produced by P. farinose significantly improved the growth of soybean in pot experiments, demonstrating a tremendous potential in agricultural application.
authors.affiliation: Key Laboratory of Systems Bioengineering, Ministry of Education, Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
authors.forename: Hong-Ji
authors.initials: HJ
authors.lastname: Zhu
authors.forename: Li-Fan
authors.initials: LF
authors.lastname: Sun
authors.forename: Yan-Fei
authors.initials: YF
authors.lastname: Zhang
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Zhang
authors.forename: Jian-Jun
authors.initials: JJ
authors.lastname: Qiao
mesh_terms: D000222:Adaptation, Physiological; D000363:Agaricales; D001483:Base Sequence; D017931:DNA Primers; D005308:Fertilizers; D010710:Phosphates / Q000378:metabolism*; D010843:Pichia / Q000378:metabolism / Q000502:physiology*; D012995:Solubility; D013312:Stress, Physiological
chemical_list: D017931:DNA Primers; D005308:Fertilizers; D010710:Phosphates