title: Immobilized smart RNA on graphene oxide nanosheets to specifically recognize and adsorb trace peptide toxins in drinking water.
abstract: The contaminations of peptide toxins in drinking water lead directly to sickness and even death in both humans and animals. A smart RNA as aptamer is covalently immobilized on graphene oxide to form a polydispersed and stable RNA-graphene oxide nanosheet. RNA-graphene oxide nanosheets can resist nuclease and natural organic matter, and specifically adsorb trace peptide toxin (microcystin-LR) in drinking water. The adsorption data fit the pseudo-second-order kinetics and the Langmuir isotherm model. The adsorption capacity of RNA-graphene oxide nanosheets decreases at extreme pH, temperature, ionic strength and natural organic matter, but it is suitable to adsorb trance pollutants in contaminated drinking water. Compared with other chemical and biological sorbents, RNA-graphene oxide nanosheets present specific and competitive adsorption, and are easily synthesized and regenerated. Aptamer (RNA) covalently immobilized on graphene oxide nanosheets is a potentially useful tool in recognizing, enriching and separating small molecules and biomacromolecules in the purification of contaminated water and the preparation of samples.
authors.affiliation: Key Laboratory of Pollution Processes and Environmental Criteria (Ministry of Education), College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China.
authors.forename: Xiangang
authors.initials: X
authors.lastname: Hu
authors.forename: Li
authors.initials: L
authors.lastname: Mu
authors.forename: Jianping
authors.initials: J
authors.lastname: Wen
authors.forename: Qixing
authors.initials: Q
authors.lastname: Zhou
mesh_terms: D000327:Adsorption; D000465:Algorithms; D001483:Base Sequence; D060766:Drinking Water / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D006108:Graphite / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008387:Marine Toxins; D052998:Microcystins / Q000737:chemistry; D018625:Microscopy, Atomic Force; D008969:Molecular Sequence Data; D049329:Nanostructures; D010455:Peptides / Q000032:analysis*; D012313:RNA / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D013816:Thermodynamics; D014118:Toxins, Biological / Q000032:analysis*; D018508:Water Purification
chemical_list: D060766:Drinking Water; D008387:Marine Toxins; D052998:Microcystins; D010455:Peptides; D014118:Toxins, Biological; D012313:RNA; D006108:Graphite; C057862:cyanoginosin LR