title: Structural implication for the impaired binding of W150A mutant LOX-1 to oxidized low density lipoprotein, OxLDL.
abstract: Lectin-like oxidized lipoprotein (OxLDL) receptor 1, LOX-1, is the major OxLDL receptor expressed on vascular endothelial cells. We have previously reported the ligand-recognition mode of LOX-1 based on the crystal structure of the ligand binding domain (C-type lectin-like domain, CTLD) and surface plasmon resonance analysis, which suggested that the functional significance of the CTLD dimer (the 'canonical' dimer) is to harbor the characteristic "basic spine" on its surface. In this study, we have identified the key inter-domain interactions in retaining the canonical CTLD dimer by X-ray structural analysis of the inactive mutant W150A CTLD. The canonical CTLD dimer forms through tight hydrophobic interactions, in which W150 engages in a lock-and-key manner and represents the main interaction. The loss of the Trp ring by mutation to Ala prevents the formation of the canonical dimer, as elucidated from docking calculations using the crystal structure of W150A CTLD. The results emphasize that the canonically formed CTLD dimer is essential for LOX-1 to bind to OxLDL, which supports our proposed view that the basic spine surface present in the correctly formed dimer plays a primal role in OxLDL recognition. This concept provides insight into the pathogenic pattern recognized by LOX-1 as a member of the pattern recognition receptors.
authors.affiliation: Department of Mathematical and Life Sciences, Hiroshima University, Higashi-Hiroshima, Japan.
authors.forename: Shogo
authors.initials: S
authors.lastname: Nakano
authors.forename: Mamoru
authors.initials: M
authors.lastname: Sugihara
authors.forename: Risato
authors.initials: R
authors.lastname: Yamada
authors.forename: Katsuo
authors.initials: K
authors.lastname: Katayanagi
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Tate
mesh_terms: D000409:Alanine / Q000737:chemistry* / Q000235:genetics; D001665:Binding Sites; D018360:Crystallography, X-Ray; D042783:Endothelial Cells / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D008077:Lipoproteins, LDL / Q000737:chemistry*; D062105:Molecular Docking Simulation; D009154:Mutation; D010084:Oxidation-Reduction; D011485:Protein Binding; D017510:Protein Folding; D055503:Protein Multimerization; D055550:Protein Stability; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics; D051127:Scavenger Receptors, Class E / Q000737:chemistry* / Q000235:genetics; D014364:Tryptophan / Q000737:chemistry* / Q000235:genetics
chemical_list: D008077:Lipoproteins, LDL; C495137:OLR1 protein, human; D011993:Recombinant Fusion Proteins; D051127:Scavenger Receptors, Class E; C099252:oxidized low density lipoprotein; D014364:Tryptophan; D000409:Alanine