title: Microsatellite markers from Ceanothus roderickii (Rhamnaceae) using next-generation sequencing technology.
abstract: OBJECTIVE
  Ceanothus roderickii is an endangered shrub endemic to California. To investigate the population genetics of this species, including the genetic consequences of population fragmentation and hybridization, 10 microsatellite markers were developed.
  
  
  RESULTS
  Using next-generation sequencing (454) data from a single C. roderickii individual, 10 microsatellite markers were developed. A group of 12 individuals representing all of the major C. roderickii populations were analyzed. All loci were found to be polymorphic, with a range from two to 12 alleles per locus. Observed heterozygosity ranged from 0.08 to 0.83 across loci. All 10 loci were also amplifiable in at least one other Ceanothus species.
  
  
  CONCLUSIONS
  Results presented here indicate the utility of our new microsatellite primers in ongoing and future studies concerning population genetics and gene flow in C. roderickii, as well as the potential applicability of these primers in similar studies on other Ceanothus species.
authors.affiliation: Department of Biology, Duke University, Durham, North Carolina 27708 USA. dylan.o.burge@gmail.com
authors.forename: Dylan O
authors.initials: DO
authors.lastname: Burge
authors.forename: Ginger
authors.initials: G
authors.lastname: Jui
authors.forename: Steven S
authors.initials: SS
authors.lastname: Kembel
authors.forename: Katherine
authors.initials: K
authors.lastname: Zhukovsky
mesh_terms: D002140:California; D018744:DNA, Plant / Q000235:genetics; D003710:Demography; D006579:Heterozygote; D018895:Microsatellite Repeats / Q000235:genetics*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D011110:Polymorphism, Genetic; D027823:Rhamnaceae / Q000235:genetics*; D013045:Species Specificity
chemical_list: D018744:DNA, Plant