title: Pseudogulbenkiania gefcensis sp. nov., isolated from soil.
abstract: A novel strain, yH16, was isolated on nutrient agar from soil samples collected at KyungHee University, Suwon City, Republic of Korea. Cells of strain yH16(T) were short rods, Gram-negative-staining, motile and non-spore-forming, with a polar flagellum. Biochemical and molecular characterization revealed that this strain was most similar to Pseudogulbenkiania subflava BP-5(T). Further 16S rRNA gene sequencing studies revealed that the new strain clustered with Pseudogulbenkiania subflava BP-5(T) (95.9 % similarity), Paludibacterium yongneupense 5YN8-15(T) (95.2 % similarity), Gulbenkiania mobilis E4FC31-5(T) (94.6 % similarity) and Chromobacterium aquaticum CC-SE-YA-1(T) (93.9 % similarity). The isolate was able to grow at 25-40 °C, 0.3-2 % NaCl and pH 5.5-7. The DNA G+C content was 65.9 ± 1.0 mol%. The predominant fatty acids were summed feature 3 (C(16 : 1)ω7c and/or iso-C(15 : 0) 2-OH) and C(16:0). Ubiquinone 8 was the major respiratory quinone. It was evident from the data obtained that the strain should be classified as a novel species of the genus Pseudogulbenkiania. The name proposed for this taxon is Pseudogulbenkiania gefcensis sp. nov., and the type strain is yH16(T) (=KCCM 90100(T) = JCM 17850(T)).
authors.affiliation: 604 GFC, B-Dong, Digital-Empire 980-3, Yeongtong-Dong, Yeongtong-Gu, Suwon-City, Gyeonggi-Do 443-813, Republic of Korea.
authors.forename: Dong-Geol
authors.initials: DG
authors.lastname: Lee
authors.affiliation: CHA Stem Cell Institute, Department of Biomedical Science, CHA University College of Medicine, Seoul 135-081, Republic of Korea.|604 GFC, B-Dong, Digital-Empire 980-3, Yeongtong-Dong, Yeongtong-Gu, Suwon-City, Gyeonggi-Do 443-813, Republic of Korea.
authors.forename: Dong-Moon
authors.initials: DM
authors.lastname: Im
authors.affiliation: 604 GFC, B-Dong, Digital-Empire 980-3, Yeongtong-Dong, Yeongtong-Gu, Suwon-City, Gyeonggi-Do 443-813, Republic of Korea.
authors.forename: HeeCheol
authors.initials: H
authors.lastname: Kang
authors.affiliation: Department of Applied Biotechnology, Ajou University, Suwon 443-749, Republic of Korea.
authors.forename: Pyeong
authors.initials: P
authors.lastname: Yun
authors.affiliation: Department of Applied Biotechnology, Ajou University, Suwon 443-749, Republic of Korea.
authors.forename: Sun-Ki
authors.initials: SK
authors.lastname: Park
authors.affiliation: Department of Applied Biotechnology, Ajou University, Suwon 443-749, Republic of Korea.
authors.forename: Seung-Su
authors.initials: SS
authors.lastname: Hyun
authors.affiliation: CHA Stem Cell Institute, Department of Biomedical Science, CHA University College of Medicine, Seoul 135-081, Republic of Korea.
authors.forename: Dong-Youn
authors.initials: DY
authors.lastname: Hwang
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009346:Neisseriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014451:Ubiquinone / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C030778:ubiquinone 8