title: Seizure activity in dogs is associated with enhanced TIMP-2 expression of microglia.
abstract: In the pathogenesis of epilepsy aberrant synaptic plasticity plays an important role. Matrix metalloproteinases (MMPs) and their inhibitors (TIMPs) are responsible for nervous tissue remodelling resulting in synaptic plasticity in the central nervous system (CNS) and might therefore be crucially involved in epileptogenesis. To assess the potential pathogenetic role of microglial MMPs and TIMPs in seizure induction, twenty-four dogs suffering from different intracranial diseases with and without seizure activity were comparatively examined. Microglial cells were isolated by density gradient centrifugation and their expression profiles of MMP-2, MMP-9, MMP-12, MMP-13, MMP-14, TIMP-1, TIMP-2, and RECK (reversion-inducing cysteine-rich protein with Kazal motifs) were examined via quantitative real-time PCR (qPCR). Interestingly, a significant up-regulation of TIMP-2 expression was found for the first time in dogs suffering from seizures. In conclusion, microglial TIMP expression might be involved in seizure generation.
authors.affiliation: Department of Small Animal Medicine and Surgery, University of Veterinary Medicine, Bünteweg 9, 30559 Hannover, Germany. veronika.stein@tiho-hannover.de
authors.forename: Veronika M
authors.initials: VM
authors.lastname: Stein
authors.forename: Sem
authors.initials: S
authors.lastname: Genini
authors.forename: Christina
authors.initials: C
authors.lastname: Puff
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Baumgärtner
authors.forename: Andrea
authors.initials: A
authors.lastname: Tipold
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000503:physiopathology*; D004285:Dogs; D020782:Matrix Metalloproteinases / Q000235:genetics / Q000502:physiology*; D017628:Microglia / Q000201:enzymology / Q000502:physiology*; D009473:Neuronal Plasticity / Q000502:physiology*; D012313:RNA / Q000737:chemistry / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D012640:Seizures / Q000503:physiopathology / Q000662:veterinary*; D018709:Statistics, Nonparametric; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000235:genetics / Q000502:physiology*
chemical_list: D019716:Tissue Inhibitor of Metalloproteinase-2; D012313:RNA; D020782:Matrix Metalloproteinases