title: Determination of the boar taint compound skatole in meat juice by means of stable isotope dilution analysis-direct immersion-solid phase microextraction-gas chromatography/mass spectrometry.
abstract: A novel SIDA-DI-SPME-GC/MS procedure for the quantitation of skatole in pork meat juice was developed and validated as a substitute for back fat sample analysis. System suitability was evaluated by determining the correlation between skatole concentrations in a subset of 38 paired meat juice and back fat samples selected from 90 fattened boars. High correlation was observed between both matrices and conclusions about the partitioning of skatole as well as of androstenone between fat and lean compartments in vivo were drawn.
authors.affiliation: University of Bonn, Department of Nutrition and Food Sciences, Bioanalytics, Bonn, Germany.
authors.forename: Jochen
authors.initials: J
authors.lastname: Fischer
authors.forename: Detert
authors.initials: D
authors.lastname: Brinkmann
authors.forename: Paul W
authors.initials: PW
authors.lastname: Elsinghorst
authors.forename: Matthias
authors.initials: M
authors.lastname: Wüst
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000736:Androstenes / Q000032:analysis / Q000378:metabolism; D000818:Animals; D001825:Body Fluid Compartments / Q000378:metabolism*; D004041:Dietary Fats / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007554:Isotopes / Q000032:analysis; D008460:Meat / Q000032:analysis*; D015203:Reproducibility of Results; D012862:Skatole / Q000032:analysis* / Q000378:metabolism; D034421:Sus scrofa / Q000378:metabolism*
chemical_list: D000736:Androstenes; D004041:Dietary Fats; D007554:Isotopes; D012862:Skatole