title: [Expression of hsa-miR-20a in human glioma tissues and its effect on the proliferation of human glioma cells in vitro].
abstract: OBJECTIVE
  To investigate miR-20a expression in human glioma and normal brain tissues and its effect on the proliferation of glioma cells in vitro.
  
  
  METHODS
  The expression of miR-20a was detected in human normal brain tissues and glioma tissues by real-time RT-PCR. miR-20a mimics were synthesized and transfected into U251 cells via liposome, and the cell proliferation were detected using MTT assay and flow cytometry.
  
  
  RESULTS
  The glioma tissues showed significantly up-regulated expression of miR-20a compared with normal brain tissues (P=0.035). The expression level of miR-20a was higher in high-grade than in low-grade gliomas. miR-20a mimics significantly enhanced the proliferation of U251 cells and the percentage of S-phase cells.
  
  
  CONCLUSIONS
  miR-20a shows potent effect in promoting the growth of glioma cells, suggesting its important role in the pathogenesis of human glioma.
authors.affiliation: Department of Neurosurgery, Qingyuan People's Hospital, Qingyuan, China.
authors.forename: Xingjun
authors.initials: X
authors.lastname: Yao
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Yuehua
authors.initials: Y
authors.lastname: Wang
authors.forename: Zhengbin
authors.initials: Z
authors.lastname: Ding
authors.forename: Wenzhong
authors.initials: W
authors.lastname: Hou
authors.forename: Minmin
authors.initials: M
authors.lastname: Zeng
authors.forename: Guangce
authors.initials: G
authors.lastname: Deng
authors.forename: Jinzhu
authors.initials: J
authors.lastname: Zhang
authors.forename: Hongfa
authors.initials: H
authors.lastname: Yang
mesh_terms: D000328:Adult; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D005910:Glioma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction; D055815:Young Adult
chemical_list: C534451:MIRN20a microRNA, human; D035683:MicroRNAs