title: Effects of parabens on apoptosis induced by serum-free medium.
abstract: Alkyl esters of p-hydroxybenzoic acids (parabens), an endocrine disrupter, are used as preservatives in cosmetics and foods. In this study, to understand the relationship between parabens and differentiation in infants, the effects of parabens on apoptosis induced by serum deprivation in PC12 cells were investigated. In addition, apoptosis-related factors were assayed. As results, a tendency toward enhancement of apoptosis was observed in the cells cultured in the serum-free medium with methylparaben, and this tendency was suggested to be related to the contents of BAD, a pro-apoptotic protein. Butylparaben did not show any tendency to enhance apoptosis.
authors.affiliation: Graduate School of Environmental Science, Hokkaido University, Sapporo, Japan.
authors.forename: Mari
authors.initials: M
authors.lastname: Egawa
authors.forename: Kentaro
authors.initials: K
authors.lastname: Aoki
authors.forename: Yongkun
authors.initials: Y
authors.lastname: Sun
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Hosokawa
authors.forename: Takeshi
authors.initials: T
authors.lastname: Saito
authors.forename: Masaaki
authors.initials: M
authors.lastname: Kurasaki
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015415:Biomarkers / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D016895:Culture Media, Serum-Free; D004797:Enzyme-Linked Immunosorbent Assay; D005520:Food Preservatives / Q000633:toxicity*; D020287:In Situ Nick-End Labeling; D016716:PC12 Cells; D010226:Parabens / Q000633:toxicity*; D011310:Preservatives, Pharmaceutical / Q000633:toxicity*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051028:bcl-2-Associated X Protein / Q000378:metabolism; D051025:bcl-Associated Death Protein / Q000378:metabolism
chemical_list: C494821:Bad protein, rat; C494828:Bax protein, rat; D015415:Biomarkers; D016895:Culture Media, Serum-Free; D005520:Food Preservatives; D010226:Parabens; D011310:Preservatives, Pharmaceutical; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D051025:bcl-Associated Death Protein; C038091:butylparaben; C015358:methylparaben; D053148:Caspase 3