title: [Correlation between antimetastatic action of Ajuga decumbens and expression of MMPs and TIMPs].
abstract: OBJECTIVE
  To investigate the anticancer and anti-metastatic effect of Ajuga decumbens extraction (HBG) on breast cancer and to clarify the effect of HBG on MMPs and TIMPs.
  
  
  METHODS
  The antitumor and antimetastic effect of HBG was determined using orthotopic 4T1 breast cancer mouse model. Western blot analysis was employed to detect the expression of associated proteins in breast cancer metastasis.
  
  
  RESULTS
  Administration with 50-200 mg x kg(-1) doses of HBG significantly reduced the tumor weight, tumor volume and numbers of lung tumor nodules in a dose-dependent manner. Tumor metastasis correlated proteins were altered following HBG treatment, MMP-2 and MMP-9 were down-regulated while TIMP-1 and TIMP-2 were up-regulated.
  
  
  CONCLUSIONS
  HBG showed anticancer and antimetastatic effect towards breast cancer through regulating the expression of MMPs and TIMPs. These data sustain our contention that HBG might be used as a potential therapeutic agent.
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Peng
authors.forename: Rong
authors.initials: R
authors.lastname: He
authors.forename: Qihua
authors.initials: Q
authors.lastname: Xu
authors.forename: Jie
authors.initials: J
authors.lastname: Gao
authors.forename: Yanli
authors.initials: Y
authors.lastname: Lu
authors.forename: Jianrong
authors.initials: J
authors.lastname: Li
mesh_terms: D031328:Ajuga; D000818:Animals; D005260:Female; D008325:Mammary Neoplasms, Experimental / Q000737:chemistry / Q000188:drug therapy* / Q000473:pathology; D020778:Matrix Metalloproteinase 2 / Q000032:analysis; D020780:Matrix Metalloproteinase 9 / Q000032:analysis; D045726:Metalloproteases / Q000032:analysis*; D051379:Mice; D008807:Mice, Inbred BALB C; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis / Q000517:prevention & control*; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use*; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000032:analysis; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000032:analysis; D019714:Tissue Inhibitor of Metalloproteinases / Q000032:analysis*
chemical_list: D010936:Plant Extracts; D019715:Tissue Inhibitor of Metalloproteinase-1; D019714:Tissue Inhibitor of Metalloproteinases; D019716:Tissue Inhibitor of Metalloproteinase-2; D045726:Metalloproteases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9