title: Method for the validation of intraspecific crosses of  Saccharomyces cerevisiae  strains by minisatellite analysis.
abstract: The crossing of  Saccharomyces  strains by spore conjugation is one of the ways to obtain new starter cultures for the fermentation industry. One of the major difficulties of this practice is the identification of the newly formed hybrids. In this work we describe an effective molecular method for the validation of Saccharomyces intraspecific crosses. The method described is based in the hypothesis that hybrids constructed by spore conjugation contain the sum of the genomes of both parental strains. As a consequence, the conjugation of spores of two yeasts showing different genomic fingerprinting profiles will result in a hybrid culture that will show the sum of both profiles. We demonstrated that the detection of polymorphism in two genes containing minisatellite-like sequences, either SED1 or AGA1, is suitable for this purpose. Using this strategy we were able to validate 15 crosses out of 162 hybridization attempts.
authors.affiliation: Department of Agriculture and Food Sciences - University of Modena and Reggio Emilia, Italy.
authors.forename: Silvio
authors.initials: S
authors.lastname: Boveri
authors.forename: Sandra
authors.initials: S
authors.lastname: Rainieri
authors.forename: Andrea
authors.initials: A
authors.lastname: Pulvirenti
mesh_terms: D015815:Cell Adhesion Molecules / Q000235:genetics; D007218:Industrial Microbiology / Q000379:methods*; D008562:Membrane Glycoproteins / Q000235:genetics; D018598:Minisatellite Repeats / Q000235:genetics*; D011110:Polymorphism, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics
chemical_list: C543384:AGA1 protein, S cerevisiae; D015815:Cell Adhesion Molecules; D008562:Membrane Glycoproteins; C077394:SED1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins