title: Developmental capacity of in vitro-matured human oocytes retrieved from polycystic ovary syndrome ovaries containing no follicles larger than 6 mm.
abstract: OBJECTIVE
  To test the developmental competence of oocytes in a nonhCG-triggered in vitro maturation (IVM) system when oocyte-cumulus complexes (OCC) are retrieved from antral follicles with a diameter of <6 mm.
  
  
  METHODS
  Prospective cohort study.
  
  
  METHODS
  Tertiary university-based referral center.
  
  
  METHODS
  From January 2010 to September 2011, 121 patients with polycystic ovaries/polycystic ovary syndrome underwent 239 IVM cycles in total. In 58 of these cycles (44 patients), all antral follicles had a diameter of <6 mm on the day of oocyte retrieval.
  
  
  METHODS
  NonhCG-triggered IVM of oocytes, fresh or vitrified/warmed embryo transfer (ET).
  
  
  METHODS
  Oocyte diameter, maturation rate, fertilization rate, embryo development and morphology, implantation rate, clinical pregnancy rate, ongoing pregnancy rate.
  
  
  RESULTS
  Oocyte retrieval yielded 16.7 OCC/cycle, and 50.8% of oocytes completed IVM. The mean oocyte diameter increased from 108.8 ± 4.3 μm to 111.9 ± 4.1 μm after IVM. Mean fertilization rate was 63.7%, and 45.4% of 2-pronuclei oocytes developed into a morphologically good-quality embryo on day 3 after intracytoplasmic sperm injection. Fresh ET resulted in two ongoing pregnancies (2/37; 5.4%). Deferred vitrified-warmed ET led to an ongoing pregnancy rate of 34.6% (9/24). Three healthy babies were born and eight pregnancies were still ongoing.
  
  
  CONCLUSIONS
  Oocytes retrieved from follicles with a diameter of <6 mm grow during a 40-hour IVM culture can acquire full competence in vitro, as illustrated by their development into healthy offspring. Endometrial quality appears to be a crucial determinant of pregnancy after nonhCG-triggered IVM.
authors.affiliation: Follicle Biology Laboratory, Universitair Ziekenhuis Brussel, Brussels, Belgium.
authors.forename: Luis
authors.initials: L
authors.lastname: Guzman
authors.forename: Carolina
authors.initials: C
authors.lastname: Ortega-Hrepich
authors.forename: Firas K
authors.initials: FK
authors.lastname: Albuz
authors.forename: Greta
authors.initials: G
authors.lastname: Verheyen
authors.forename: Paul
authors.initials: P
authors.lastname: Devroey
authors.forename: Johan
authors.initials: J
authors.lastname: Smitz
authors.forename: Michel
authors.initials: M
authors.lastname: De Vos
mesh_terms: D000328:Adult; D048429:Cell Size; D004624:Embryo Transfer / Q000379:methods; D005260:Female; D006801:Humans; D059471:In Vitro Oocyte Maturation Techniques / Q000379:methods*; D007231:Infant, Newborn; D054315:Oocyte Retrieval / Q000379:methods*; D009865:Oocytes / Q000254:growth & development* / Q000473:pathology; D006080:Ovarian Follicle / Q000254:growth & development* / Q000473:pathology; D011085:Polycystic Ovary Syndrome / Q000473:pathology / Q000628:therapy*; D011247:Pregnancy; D018873:Pregnancy Rate / Q000639:trends