title: Human IgG Fc-glycosylation profiling reveals associations with age, sex, female sex hormones and thyroid cancer.
abstract: IgG functions rely on interactions of the Fc region with other proteins, which are optimized by tailoring of a conserved N-linked glycosylation at Asn-297. We conducted a study involving 735 control individuals and 138 thyroid cancer patients. Here we demonstrated that previously described age-related change in Fc-glycosylation was further characterized by definite sex specificity. In females, the incidences of most of glycosylated forms began to pose characteristic changes at ages of puberty or menopause. In addition, glycan-glycan relationships existed extensively within Fc glycosylation, which were characterized to be altered upon different states of subjects, such as age, sex and thyroid cancer. In thyroid cancer patients, detailed comparison of glycosylation incidences with control individuals yielded insight into aberrant change in IgG(1) Fc-glycosylation. This aberrant pattern was also featured by remarkable specificities of both age and sex. The receiver operating characteristic curve analysis was used to determine diagnostic values of Fc glycosylation. Finally, clinical measurement of two major female sex hormones estradiol and progesterone was conducted to determine potential associations of hormones with IgG Fc glycosylation. This study provided an important view to the associations of IgG Fc N-linked glycosylation with age, sex, female sex hormones and thyroid cancer. This article is part of a Special Issue entitled: Proteomics: The clinical link.
authors.affiliation: Department of Biophysics and Structural Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, PR China.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Chen
authors.forename: Yanmin
authors.initials: Y
authors.lastname: Wang
authors.forename: Ling
authors.initials: L
authors.lastname: Qiu
authors.forename: Xuzhen
authors.initials: X
authors.lastname: Qin
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Wang
authors.forename: Yanying
authors.initials: Y
authors.lastname: Wang
authors.forename: Gaoguang
authors.initials: G
authors.lastname: Song
authors.forename: Fang
authors.initials: F
authors.lastname: Li
authors.forename: Yumei
authors.initials: Y
authors.lastname: Guo
authors.forename: Fenjie
authors.initials: F
authors.lastname: Li
authors.forename: Shuai
authors.initials: S
authors.lastname: Guo
authors.forename: Zhili
authors.initials: Z
authors.lastname: Li
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D002277:Carcinoma / Q000097:blood / Q000175:diagnosis* / Q000453:epidemiology / Q000378:metabolism; D016022:Case-Control Studies; D002648:Child; D005260:Female; D006031:Glycosylation; D012739:Gonadal Steroid Hormones / Q000032:analysis / Q000097:blood*; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000032:analysis / Q000097:blood / Q000378:metabolism*; D007074:Immunoglobulin G / Q000032:analysis / Q000097:blood*; D008297:Male; D055442:Metabolome; D008875:Middle Aged; D012737:Sex Factors; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013964:Thyroid Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000453:epidemiology / Q000378:metabolism; D055815:Young Adult; D006023:Glycoproteins
chemical_list: D012739:Gonadal Steroid Hormones; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; C046179:glycosylated IgG; D006023:Glycoproteins