title: Relative expression of TAp73 and ΔNp73 isoforms.
abstract: The transcription factor p73 belongs to the p53 family of tumour suppressors and similar to other family members, transcribed as different isoforms with opposing pro- and anti-apoptotic functions. Unlike p53, p73 mutations are extremely rare in cancers. Instead, the pro-apoptotic activities of transcriptionally active p73 isoforms are commonly inhibited by over-expression of the dominant negative p73 isoforms. Therefore the relative ratio of different p73 isoforms is critical for the cellular response to a chemotherapeutic agent. Here, we analysed the expression of N-terminal p73 isoforms in cell lines and mouse tissues. Our data showed that the transcriptionally competent TAp73 isoform is abundantly expressed in cancer cell lines compared to the dominant negative ΔNp73 isoform. Interestingly, we detected higher levels of ΔNp73 in some mouse tissues, suggesting that ΔNp73 may have a physiological role in these tissues.
authors.affiliation: University of Southampton, Cancer Sciences Unit, Somers Cancer Research Building, Southampton, UK.
authors.forename: Franco
authors.initials: F
authors.lastname: Conforti
authors.forename: Ai Li
authors.initials: AL
authors.lastname: Yang
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Agostini
authors.forename: Alessandro
authors.initials: A
authors.lastname: Rufini
authors.forename: Paola
authors.initials: P
authors.lastname: Tucci
authors.forename: Maria Victoria
authors.initials: MV
authors.lastname: Nicklison-Chirou
authors.forename: Francesca
authors.initials: F
authors.lastname: Grespi
authors.forename: Tania
authors.initials: T
authors.lastname: Velletri
authors.forename: Richard A
authors.initials: RA
authors.lastname: Knight
authors.forename: Gerry
authors.initials: G
authors.lastname: Melino
authors.forename: Berna S
authors.initials: BS
authors.lastname: Sayan
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D045325:HCT116 Cells; D057809:HEK293 Cells; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D051379:Mice; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D020033:Protein Isoforms; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D014158:Transcription, Genetic; D014162:Transfection; D000072160:Tumor Protein p73; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger; C000606460:TP73 protein, human; C000606368:Trp73 protein, mouse; D000072160:Tumor Protein p73; D025521:Tumor Suppressor Proteins; C000606369:delta Np73 protein, human