title: [Lentivirus-mediated LOX-1-RNA interference attenuates oxidative stress-induced apoptosis in myocardial cells].
abstract: OBJECTIVE
  To construct a lectin-like oxidized low-density lipoprotein receptor (LOX-1) RNA interference (RNAi) lentivirus and explore the role of LOX-1 in H(2)O(2)-induced apoptosis of rat myocardial cells.
  
  
  METHODS
  LOX-1 shRNA sequence was synthesized and cloned into pLentiLox3.7 (pLL3.7) lentiviral vector to construct the lentiviral vector pLL3.7-LOX1. The lentiviral vectors (pLL3.7 and pLL3.7-LOX1) and the packaging vectors dR8.9 and VSVG were co-transfected into 293FT cells for packaging the lentivirus. H9C2 myocardial cells were infected by the lentiviruses to observe the inhibitory rate of LOX-1 on H(2)O(2)-induced apoptosis of H9C2 cells by RT-PCR, CCK-8, and Hochest33258 staining.
  
  
  RESULTS
  Double restriction enzyme digestion and DNA sequencing confirmed correct insertion of the sequence. Suppression of LOX-1 by the lentivirus attenuated H(2)O(2)-induced cell viability reduction and apoptosis in the myocardial cells (P<0.01).
  
  
  CONCLUSIONS
  LOX-1 activation may play an important role in H(2)O(2)-induced apoptosis of rat myocardial cells.
authors.affiliation: Department of Traditional Chinese Medicine, Southern Medical University, Guangzhou, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.forename: Jiacheng
authors.initials: J
authors.lastname: Huang
authors.forename: Yingchun
authors.initials: Y
authors.lastname: Zhou
authors.forename: Xuegang
authors.initials: X
authors.lastname: Sun
authors.forename: Wenbi
authors.initials: W
authors.lastname: Zeng
authors.forename: Xing
authors.initials: X
authors.lastname: Li
authors.forename: Shuwen
authors.initials: S
authors.lastname: Zang
authors.forename: Wenwen
authors.initials: W
authors.lastname: Hao
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002478:Cells, Cultured; D005822:Genetic Vectors / Q000235:genetics; D006861:Hydrogen Peroxide; D016086:Lentivirus / Q000235:genetics; D032383:Myocytes, Cardiac / Q000166:cytology*; D018384:Oxidative Stress; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D051381:Rats; D051127:Scavenger Receptors, Class E / Q000235:genetics*
chemical_list: C495136:OLR1 protein, rat; D034741:RNA, Small Interfering; D051127:Scavenger Receptors, Class E; D006861:Hydrogen Peroxide