title: Molecular biology of Pasteurella multocida toxin.
abstract: Pasteurella multocida toxin (PMT) is the causative agent of progressive atrophic rhinitis in swine. The 146 kDa single-chain toxin harbours discrete domains important for receptor binding, internalisation and biological activity. The molecular basis of the toxin's activity is the deamidation of a specific glutamine residue in the α-subunit of heterotrimeric G proteins. This results in an inhibition of the inherent GTPase activity leading to a constitutively active phenotype of the G protein. Due to the ability of the toxin to act on various families of heterotrimeric G proteins, a large subset of signal transduction pathways is stimulated.
authors.affiliation: Institut für Experimentelle und Klinische Pharmakologie und Toxikologie, Albert-Ludwigs-Universität Freiburg, Albertstr. 25, 79104, Freiburg, Germany. joachim.orth@pharmakol.uni-freiburg.de
authors.forename: Joachim H C
authors.initials: JH
authors.lastname: Orth
authors.forename: Klaus
authors.initials: K
authors.lastname: Aktories
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001427:Bacterial Toxins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D005973:Glutamine / Q000235:genetics / Q000378:metabolism; D020962:Heterotrimeric GTP-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008958:Models, Molecular; D010326:Pasteurella Infections / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D016979:Pasteurella multocida / Q000235:genetics* / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012222:Rhinitis, Atrophic / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D015398:Signal Transduction / Q000235:genetics; D013552:Swine; D015854:Up-Regulation
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; C062258:Pasteurella multocida toxin; D005973:Glutamine; D020962:Heterotrimeric GTP-Binding Proteins