title: Functional characterization of the p53 binding site in the human PYNOD promoter.
abstract: Many members of the NOD-like receptor (NLR) family play important roles in pathogen recognition and inflammation. However, human PYNOD, an NLR-like protein consisting of a pyrin domain and a nucleotidebinding and oligomerization domain (NOD), has been reported to inhibit inflammatory signals. Using bioinformatics, we found a completely preserved canonical p53 binding site in the PYNOD core promoter (-228 to -237 bp) both in humans and in chimpanzees. In this study, we investigated the characterization and biologic function of this binding site in vitro. The results show that either deletion of the p53 binding elements within the PYNOD promoter or treatment with p53 inhibitor (PFT-α) could significantly reduce PYNOD promoter activity and PYNOD expression as detected by the enhanced green fluorescent protein (EGFP) reporter system, reverse transcription-polymerase chain reaction, and Western blot respectively. Furthermore, the chromatin immunoprecipitation (ChIP) method confirmed that p53 could bind to the PYNOD promoter. Our findings suggest that the p53 binding site plays a positive role in regulating PYNOD gene expression, which may maintain an efficient balance between defense and self-inflicted injury in respond to pathogen invasion.
authors.affiliation: Department of Pathophysiology, Key Laboratory of State Administration of Traditional Chinese Medicine of the People's Republic of China, Medical College, Jinan University, Guangdong, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zeng
authors.forename: Daxiang
authors.initials: D
authors.lastname: Lu
authors.forename: Qingliang
authors.initials: Q
authors.lastname: Tang
authors.forename: Li
authors.initials: L
authors.lastname: Tian
authors.forename: Huadong
authors.initials: H
authors.lastname: Wang
authors.forename: Suisheng
authors.initials: S
authors.lastname: Tang
authors.forename: Chaofeng
authors.initials: C
authors.lastname: Hu
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D051017:Apoptosis Regulatory Proteins; D001483:Base Sequence; D052160:Benzothiazoles / Q000494:pharmacology; D001665:Binding Sites; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D015870:Gene Expression; D023061:Gene Order; D006801:Humans; D059372:Nucleotide Motifs; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism; D011995:Recombination, Genetic; D017384:Sequence Deletion; D014050:Toluene / Q000031:analogs & derivatives / Q000494:pharmacology; D016159:Tumor Suppressor Protein p53 / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D051017:Apoptosis Regulatory Proteins; D052160:Benzothiazoles; D002352:Carrier Proteins; C494352:NLRP10 protein, human; D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53; D014050:Toluene; C121565:pifithrin