title: OxLDL-targeted iron oxide nanoparticles for in vivo MRI detection of perivascular carotid collar induced atherosclerotic lesions in ApoE-deficient mice.
abstract: Atherosclerotic disease is a leading cause of morbidity and mortality in developed countries, and oxidized LDL (OxLDL) plays a key role in the formation, rupture, and subsequent thrombus formation in atherosclerotic plaques. In the current study, anti-mouse OxLDL polyclonal antibody and nonspecific IgG antibody were conjugated to polyethylene glycol-coated ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles, and a carotid perivascular collar model in apolipoprotein E-deficient mice was imaged at 7.0 Tesla MRI before contrast administration and at 8 h and 24 h after injection of 30 mg Fe/kg. The results showed MRI signal loss in the carotid atherosclerotic lesions after administration of targeted anti-OxLDL-USPIO at 8 h and 24 h, which is consistent with the presence of the nanoparticles in the lesions. Immunohistochemistry confirmed the colocalization of the OxLDL/macrophages and iron oxide nanoparticles. The nonspecific IgG-USPIO, unconjugated USPIO nanoparticles, and competitive inhibition groups had limited signal changes (p < 0.05). This report shows that anti-OxLDL-USPIO nanoparticles can be used to directly detect OxLDL and image atherosclerotic lesions within 24 h of nanoparticle administration and suggests a strategy for the therapeutic evaluation of atherosclerotic plaques in vivo.
authors.affiliation: Jiangsu Key Laboratory of Molecular and Functional Imaging, Department of Radiology, Zhongda Hospital, Medical School, Southeast University, Nanjing, China.
authors.forename: Song
authors.initials: S
authors.lastname: Wen 文颂
authors.affiliation: Jiangsu Key Laboratory of Molecular and Functional Imaging, Department of Radiology, Zhongda Hospital, Medical School, Southeast University, Nanjing, China.
authors.forename: Dong-Fang
authors.initials: DF
authors.lastname: Liu 柳东芳
authors.affiliation: Jiangsu Key Laboratory of Molecular and Functional Imaging, Atherosclerosis Research Center, Nanjing Medical University, Nanjing, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Liu 刘振
authors.affiliation: Department of Biomedical Engineering, Emory University/Georgia Institute of Technology, Atlanta, GA.
authors.forename: Steven
authors.initials: S
authors.lastname: Harris
authors.affiliation: Department of Cardiology, Zhongda Hospital, Southeast University, Nanjing, China.
authors.forename: Yu-Yu
authors.initials: YY
authors.lastname: Yao 姚玉宇
authors.affiliation: Jiangsu Key Laboratory for Biomaterials and Devices, State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Ding 丁琪
authors.affiliation: Jiangsu Key Laboratory of Molecular and Functional Imaging, Department of Radiology, Zhongda Hospital, Medical School, Southeast University, Nanjing, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Nie 聂芳
authors.affiliation: Jiangsu Key Laboratory of Molecular and Functional Imaging, Department of Radiology, Zhongda Hospital, Medical School, Southeast University, Nanjing, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Lu 卢瞳
authors.affiliation: Jiangsu Key Laboratory of Molecular and Functional Imaging, Department of Radiology, Zhongda Hospital, Medical School, Southeast University, Nanjing, China.
authors.forename: Hua-Jun
authors.initials: HJ
authors.lastname: Chen 陈华俊
authors.affiliation: Jiangsu Key Laboratory of Molecular and Functional Imaging, Department of Radiology, Zhongda Hospital, Medical School, Southeast University, Nanjing, China.
authors.forename: Yan-Li
authors.initials: YL
authors.lastname: An 安艳丽
authors.affiliation: Jiangsu Key Laboratory of Molecular and Functional Imaging, Department of Radiology, Zhongda Hospital, Medical School, Southeast University, Nanjing, China.
authors.forename: Feng-Chao
authors.initials: FC
authors.lastname: Zang 臧凤超
authors.affiliation: Jiangsu Key Laboratory of Molecular and Functional Imaging, Department of Radiology, Zhongda Hospital, Medical School, Southeast University, Nanjing, China. Electronic address: gjteng@vip.sina.com.
authors.forename: Gao-Jun
authors.initials: GJ
authors.lastname: Teng 滕皋军
mesh_terms: D000818:Animals; D001057:Apolipoproteins E / Q000172:deficiency*; D002339:Carotid Arteries; D003250:Constriction; D003287:Contrast Media / Q000737:chemistry / Q000378:metabolism; D005290:Ferric Compounds / Q000737:chemistry / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D053758:Nanoparticles; D058226:Plaque, Atherosclerotic / Q000175:diagnosis* / Q000209:etiology / Q000378:metabolism; D011092:Polyethylene Glycols / Q000737:chemistry
chemical_list: D001057:Apolipoproteins E; D003287:Contrast Media; D005290:Ferric Compounds; D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; C000499:ferric oxide; D011092:Polyethylene Glycols