title: [Genistein attenuates monocrotaline-induced pulmonary arterial hypertension in rats by up-regulating heme oxygenase-1 expression].
abstract: OBJECTIVE
  To study the effect of genistein on the expression of heme oxygenase-1 (HO-1) in rats with pulmonary arterial hypertension (PAH) induced by monocrotaline (MCT).
  
  
  METHODS
  Sixty male Sprague-Dawley rats were randomly divided into 4 groups (n=15), namely the control group, model group, low-dose (20 µg/kg) genistein group and high-dose (80 µg/kg) genistein group. The hemodynamic parameters were measured and the remodeling of pulmonary small arteries was observed by electron microscope (EM). The expression of HO-1 in the lung tissues were detected by Western blotting.
  
  
  RESULTS
  Compared with the model group, genistein treatment significantly reduced the elevated mean pulmonary arterial pressure, improved the right ventricular hypertrophy index, and increased the expression of HO-1 in a dose-dependent manner.
  
  
  CONCLUSIONS
  Genistein attentuates pulmonary arterial hypertension in MCT-treated rats possibly by up-regulation of HO-1 in the lung tissues.
authors.affiliation: Department of Respiratory Medicine, Second Affiliated Hospital of Chongqing Medical University, Chongqing, China. zyk0421@126.com
authors.forename: Yukun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Daoxin
authors.initials: D
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Zhu
authors.forename: Changyi
authors.initials: C
authors.lastname: Li
mesh_terms: D000818:Animals; D019833:Genistein / Q000494:pharmacology* / Q000627:therapeutic use; D006419:Heme Oxygenase (Decyclizing) / Q000378:metabolism*; D006976:Hypertension, Pulmonary / Q000139:chemically induced / Q000188:drug therapy* / Q000201:enzymology; D008168:Lung / Q000201:enzymology* / Q000473:pathology; D008297:Male; D016686:Monocrotaline; D051381:Rats; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D016686:Monocrotaline; D019833:Genistein; D006419:Heme Oxygenase (Decyclizing); C491523:Hmox1 protein, rat