title: Glycomic and proteomic profiling of pancreatic cyst fluids identifies hyperfucosylated lactosamines on the N-linked glycans of overexpressed glycoproteins.
abstract: Pancreatic cancer is now the fourth leading cause of cancer deaths in the United States, and it is associated with an alarmingly low 5-year survival rate of 5%. However, a patient's prognosis is considerably improved when the malignant lesions are identified at an early stage of the disease and removed by surgical resection. Unfortunately, the absence of a practical screening strategy and clinical diagnostic test for identifying premalignant lesions within the pancreas often prevents early detection of pancreatic cancer. To aid in the development of a molecular screening system for early detection of the disease, we have performed glycomic and glycoproteomic profiling experiments on 21 pancreatic cyst fluid samples, including fluids from mucinous cystic neoplasms and intraductal papillary mucinous neoplasms, two types of mucinous cysts that are considered high risk to undergo malignant transformation. A total of 80 asparagine-linked (N-linked) glycans, including high mannose and complex structures, were identified. Of special interest was a series of complex N-linked glycans containing two to six fucose residues, located predominantly as substituents on β-lactosamine extensions. Following the observation of these "hyperfucosylated" glycans, bottom-up proteomics experiments utilizing a label-free quantitative approach were applied to the investigation of two sets of tryptically digested proteins derived from the cyst fluids: 1) all soluble proteins in the raw samples and 2) a subproteome of the soluble cyst fluid proteins that were selectively enriched for fucosylation through the use of surface-immobilized Aleuria aurantia lectin. A comparative analysis of these two proteomic data sets identified glycoproteins that were significantly enriched by lectin affinity. Several candidate glycoproteins that appear hyperfucosylated were identified, including triacylglycerol lipase and pancreatic α-amylase, which were 20- and 22-fold more abundant, respectively, following A. aurantia lectin enrichment.
authors.affiliation: Chemistry Department of Indiana University, Bloomington, Indiana 47405, USA.
authors.forename: Benjamin F
authors.initials: BF
authors.lastname: Mann
authors.forename: John A
authors.initials: JA
authors.lastname: Goetz
authors.forename: Michael G
authors.initials: MG
authors.lastname: House
authors.forename: C Max
authors.initials: CM
authors.lastname: Schmidt
authors.forename: Milos V
authors.initials: MV
authors.lastname: Novotny
mesh_terms: D000368:Aged; D000606:Amino Sugars / Q000032:analysis* / Q000378:metabolism; D044963:Biopsy, Fine-Needle; D002846:Chromatography, Affinity; D020420:Cyst Fluid / Q000737:chemistry; D018291:Cystadenoma, Mucinous / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D042241:Early Diagnosis; D005260:Female; D005643:Fucose / Q000378:metabolism; D015870:Gene Expression; D054794:Glycomics; D006023:Glycoproteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D037102:Lectins; D008049:Lipase / Q000235:genetics / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D010179:Pancreas / Q000378:metabolism / Q000473:pathology; D010181:Pancreatic Cyst / Q000378:metabolism* / Q000473:pathology; D010190:Pancreatic Neoplasms / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D011134:Polysaccharides / Q000032:analysis*; D040901:Proteomics; D000516:alpha-Amylases / Q000235:genetics / Q000378:metabolism
chemical_list: D000606:Amino Sugars; D006023:Glycoproteins; D037102:Lectins; D011134:Polysaccharides; C064549:lectin, Aleuria aurantia; D005643:Fucose; D008049:Lipase; D000516:alpha-Amylases