title: Afa/Dr diffusely adhering Escherichia coli strain C1845 induces neutrophil extracellular traps that kill bacteria and damage human enterocyte-like cells.
abstract: We recently documented the neutrophil response to enterovirulent diffusely adherent Escherichia coli expressing Afa/Dr fimbriae (Afa/Dr DAEC), using the human myeloid cell line PLB-985 differentiated into fully mature neutrophils. Upon activation, particularly during infections, neutrophils release neutrophil extracellular traps (NETs), composed of a nuclear DNA backbone associated with antimicrobial peptides, histones, and proteases, which entrap and kill pathogens. Here, using fluorescence microscopy and field emission scanning electron microscopy, we observed NET production by PLB-985 cells infected with the Afa/Dr wild-type (WT) E. coli strain C1845. We found that these NETs were able to capture, immobilize, and kill WT C1845 bacteria. We also developed a coculture model of human enterocyte-like Caco-2/TC7 cells and PLB-985 cells previously treated with WT C1845 and found, for the first time, that the F-actin cytoskeleton of enterocyte-like cells is damaged in the presence of bacterium-induced NETs and that this deleterious effect is prevented by inhibition of protease release. These findings provide new insights into the neutrophil response to bacterial infection via the production of bactericidal NETs and suggest that NETs may damage the intestinal epithelium, particularly in situations such as inflammatory bowel diseases.
authors.affiliation: INSERM, UMR-S 996, Cytokines, Chemokines and Immunopathology, Châtenay-Malabry, France.
authors.forename: Viviana
authors.initials: V
authors.lastname: Marin-Esteban
authors.forename: Isabelle
authors.initials: I
authors.lastname: Turbica
authors.forename: Guillaume
authors.initials: G
authors.lastname: Dufour
authors.forename: Nicolas
authors.initials: N
authors.lastname: Semiramoth
authors.forename: Aude
authors.initials: A
authors.lastname: Gleizes
authors.forename: Roseline
authors.initials: R
authors.lastname: Gorges
authors.forename: Isabelle
authors.initials: I
authors.lastname: Beau
authors.forename: Alain L
authors.initials: AL
authors.lastname: Servin
authors.forename: Vanessa
authors.initials: V
authors.lastname: Lievin-Le Moal
authors.forename: Catherine
authors.initials: C
authors.lastname: Sandré
authors.forename: Sylvie
authors.initials: S
authors.lastname: Chollet-Martin
mesh_terms: D018830:Adhesins, Escherichia coli / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D018920:Coculture Techniques; D020895:Enterocytes / Q000166:cytology*; D004926:Escherichia coli / Q000378:metabolism* / Q000648:ultrastructure; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D006657:Histones / Q000378:metabolism; D006801:Humans; D009504:Neutrophils / Q000502:physiology*; D010447:Peptide Hydrolases / Q000378:metabolism; D023084:alpha-Defensins / Q000378:metabolism*
chemical_list: D018830:Adhesins, Escherichia coli; D029968:Escherichia coli Proteins; D006657:Histones; D023084:alpha-Defensins; D010447:Peptide Hydrolases