title: Digested Ara h 1 loses sensitizing capacity when separated into fractions.
abstract: The major peanut allergen Ara h 1 is an easily digestible protein under physiological conditions. The present study revealed that pepsin digestion products of Ara h 1 retained the sensitizing potential in a Brown Norway rat model, while this sensitizing capacity was lost by separating the digest into fractions by gel permeation chromatography. Protein chemical analysis showed that the peptide composition as well as the aggregation profiles of the fractions of Ara h 1 digest differed from that of the whole pool. These results indicate that the sensitizing capacity of digested Ara h 1 is a consequence of the peptides being in an aggregated state resembling the intact molecule or that most peptides of the digests need to be present in the same solution, having a synergistic or adjuvant effect and thereby augmenting the immune response against other peptides.
authors.affiliation: National Food Institute, Division of Toxicology and Risk Assessment, Technical University of Denmark, Mørkhøj Bygade 19, DK-2860 Søborg, Denmark. kalb@food.dtu.dk
authors.forename: Katrine L
authors.initials: KL
authors.lastname: Bøgh
authors.forename: Vibeke
authors.initials: V
authors.lastname: Barkholt
authors.forename: Neil M
authors.initials: NM
authors.lastname: Rigby
authors.forename: E N Clare
authors.initials: EN
authors.lastname: Mills
authors.forename: Charlotte B
authors.initials: CB
authors.lastname: Madsen
mesh_terms: D000818:Animals; D052179:Antigens, Plant / Q000737:chemistry* / Q000276:immunology*; D005591:Chemical Fractionation; D002850:Chromatography, Gel; D006023:Glycoproteins / Q000737:chemistry* / Q000276:immunology*; D006801:Humans; D008565:Membrane Proteins; D021183:Peanut Hypersensitivity / Q000276:immunology*; D010455:Peptides / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D010940:Plant Proteins / Q000737:chemistry* / Q000276:immunology*; D051381:Rats
chemical_list: D052179:Antigens, Plant; C500889:Ara h 1 protein, Arachis hypogaea; D006023:Glycoproteins; D008565:Membrane Proteins; D010455:Peptides; D010940:Plant Proteins