title: Diverse population of a new bipartite begomovirus infecting tomato crops in Uruguay.
abstract: Several isolates of a novel begomovirus were characterized from tomato samples collected in northern Uruguay exhibiting disease symptoms associated with Bemisia tabaci infestations. Analysis of full-length sequences of DNA-A and DNA-B components revealed the presence of a new begomovirus with the typical genome organization of a New World begomovirus, for which the name tomato rugose yellow leaf curl virus (ToRYLCV) is proposed. A high degree of nucleotide sequence diversity was found for both components, suggesting the presence of a diverse virus population. Recombination analysis suggested relationships of ToRYLCV to begomoviruses reported from the New World. Although common regions from DNA-As and DNA-Bs were surprisingly divergent for a cognate pair, a DNA-A and DNA-B pair cloned from one sample were infectious in Nicotiana benthamiana and tomato and reproduced symptoms observed in field-infected tomato plants, suggesting that ToRYLCV is the causal agent of the disease observed. This is the first report of a begomovirus infecting tomato crops in Uruguay and of the presence of begomovirus in this country.
authors.affiliation: Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora", Consejo Superior de Investigaciones Científicas, Algarrobo-Costa, Málaga, Spain.
authors.forename: Belén
authors.initials: B
authors.lastname: Márquez-Martín
authors.forename: Diego
authors.initials: D
authors.lastname: Maeso
authors.forename: Ada
authors.initials: A
authors.lastname: Martínez-Ayala
authors.forename: Roberto
authors.initials: R
authors.lastname: Bernal
authors.forename: M
authors.initials: M
authors.lastname: Teresa Federici
authors.forename: Paul
authors.initials: P
authors.lastname: Vincelli
authors.forename: Jesús
authors.initials: J
authors.lastname: Navas-Castillo
authors.forename: Enrique
authors.initials: E
authors.lastname: Moriones
mesh_terms: D053839:Begomovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D044822:Biodiversity; D018551:Solanum lycopersicum / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D014026:Nicotiana / Q000821:virology; D014583:Uruguay