title: Alterations in differentially expressed genes by exposure to a mixture of carcinogenic polycyclic aromatic hydrocarbons in the liver of Oryzias latipes.
abstract: The effects of a mixture of carcinogenic polycyclic aromatic hydrocarbons (cPAHs) on transcriptional responses in the liver of medaka, Oryzias latipes, were investigated by identifying differentially expressed genes (DEGs). Five DEGs were identified as cytochrome P450 2P1 (CYP450 2P1), malate dehydrogenase, anti-thrombin III, NADH dehydrogenase subunit 4, and transferrin. These DEGs were quantified by real-time polymerase chain reaction. Only CYP450 2P1 mRNA was found to be upregulated by exposure to cPAHs mixture, suggesting that CYP450 2P1 mRNA can be a potential marker for prediction of the biological effects of a mixture of cPAHs on fish.
authors.affiliation: Department of Biological Sciences, Pusan National University, Kumjeong-gu, Pusan, Republic of Korea.
authors.forename: Jeong Hwan
authors.initials: JH
authors.lastname: Oh
authors.forename: Hyo-Bang
authors.initials: HB
authors.lastname: Moon
authors.forename: Eun Sang
authors.initials: ES
authors.lastname: Choe
mesh_terms: D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D029941:Fish Proteins / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D009990:Oryzias / Q000378:metabolism*; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity*; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D029941:Fish Proteins; D011084:Polycyclic Aromatic Hydrocarbons; D012333:RNA, Messenger; D014874:Water Pollutants, Chemical; D003577:Cytochrome P-450 Enzyme System