title: Estradiol increases hematopoietic stem and progenitor cells independent of its actions on bone.
abstract: Hematopoietic stem and progenitor cells reside in vascular and endosteal niches in the bone marrow. Factors affecting bone remodeling were reported to influence numbers and mobilization of hematopoietic stem cells. We therefore analyzed the effects of estradiol acting anabolic on bone integrity. Here we observe that estradiol increases progenitor cell numbers in the vascular but not in the endosteal compartment independent of its estrogen receptor α-dependent anabolic bone effects. Hematopoietic progenitors capable of reconstituting lethally irradiated mice are increased by enhanced cell cycle entry, leading to a diminished long-term reconstitution potential after serial transplantation. We demonstrate that estradiol action on stromal cells potently favors hematopoietic progenitor/stem cell frequency accompanied by enhanced expression of cell adhesion molecules. Finally, estradiol treatment enhances retention of hematopoietic stem cells in the vascular niche of the bone marrow. We describe for the first time the mechanism of estrogen action on hematopoietic stem and progenitor cells.
authors.affiliation: Leibniz-Institute for Age Research – Fritz Lipmann Institute (FLI), Germany.
authors.forename: Anett
authors.initials: A
authors.lastname: Illing
authors.forename: Peng
authors.initials: P
authors.lastname: Liu
authors.forename: Susanne
authors.initials: S
authors.lastname: Ostermay
authors.forename: Arndt
authors.initials: A
authors.lastname: Schilling
authors.forename: Gerald
authors.initials: G
authors.lastname: de Haan
authors.forename: Andree
authors.initials: A
authors.lastname: Krust
authors.forename: Michael
authors.initials: M
authors.lastname: Amling
authors.forename: Pierre
authors.initials: P
authors.lastname: Chambon
authors.forename: Thorsten
authors.initials: T
authors.lastname: Schinke
authors.forename: Jan P
authors.initials: JP
authors.lastname: Tuckermann
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects; D001842:Bone and Bones / Q000187:drug effects*; D004958:Estradiol / Q000494:pharmacology*; D047628:Estrogen Receptor alpha / Q000235:genetics / Q000378:metabolism; D047629:Estrogen Receptor beta / Q000235:genetics / Q000378:metabolism; D005260:Female; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D055153:Stem Cell Niche / Q000187:drug effects
chemical_list: D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D004958:Estradiol