title: Combined linkage and association mapping reveals CYCD5;1 as a quantitative trait gene for endoreduplication in Arabidopsis.
abstract: Endoreduplication is the process where a cell replicates its genome without mitosis and cytokinesis, often followed by cell differentiation. This alternative cell cycle results in various levels of endoploidy, reaching 4× or higher one haploid set of chromosomes. Endoreduplication is found in animals and is widespread in plants, where it plays a major role in cellular differentiation and plant development. Here, we show that variation in endoreduplication between Arabidopsis thaliana accessions Columbia-0 and Kashmir is controlled by two major quantitative trait loci, ENDO-1 and ENDO-2. A local candidate gene association analysis in a set of 87 accessions, combined with expression analysis, identified CYCD5;1 as the most likely candidate gene underlying ENDO-2, operating as a rate-determining factor of endoreduplication. In accordance, both the overexpression and silencing of CYCD5;1 were effective in changing DNA ploidy levels, confirming CYCD5;1 to be a previously undescribed quantitative trait gene underlying endoreduplication in Arabidopsis.
authors.affiliation: Department of Plant Systems Biology, VIB, B-9052 Ghent,  Belgium.
authors.forename: Roel
authors.initials: R
authors.lastname: Sterken
authors.forename: Raphaël
authors.initials: R
authors.lastname: Kiekens
authors.forename: Joanna
authors.initials: J
authors.lastname: Boruc
authors.forename: Fanghong
authors.initials: F
authors.lastname: Zhang
authors.forename: Annelies
authors.initials: A
authors.lastname: Vercauteren
authors.forename: Ilse
authors.initials: I
authors.lastname: Vercauteren
authors.forename: Lien
authors.initials: L
authors.lastname: De Smet
authors.forename: Stijn
authors.initials: S
authors.lastname: Dhondt
authors.forename: Dirk
authors.initials: D
authors.lastname: Inzé
authors.forename: Lieven
authors.initials: L
authors.lastname: De Veylder
authors.forename: Eugenia
authors.initials: E
authors.lastname: Russinova
authors.forename: Marnik
authors.initials: M
authors.lastname: Vuylsteke
mesh_terms: D000483:Alleles; D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000502:physiology*; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics; D017343:Genes, Plant; D005838:Genotype; D006239:Haplotypes; D007700:Kinetics; D008126:Lod Score; D008957:Models, Genetic; D010641:Phenotype; D018521:Plant Physiological Phenomena; D011003:Ploidies; D011110:Polymorphism, Genetic; D040641:Quantitative Trait Loci
chemical_list: D029681:Arabidopsis Proteins