title: Hemodialysis removes uremic toxins that alter the biological actions of endothelial cells.
abstract: Chronic kidney disease is linked to systemic inflammation and to an increased risk of ischemic heart disease and atherosclerosis. Endothelial dysfunction associates with hypertension and vascular disease in the presence of chronic kidney disease but the mechanisms that regulate the activation of the endothelium at the early stages of the disease, before systemic inflammation is established remain obscure. In the present study we investigated the effect of serum derived from patients with chronic kidney disease either before or after hemodialysis on the activation of human endothelial cells in vitro, as an attempt to define the overall effect of uremic toxins at the early stages of endothelial dysfunction. Our results argue that uremic toxins alter the biological actions of endothelial cells and the remodelling of the extracellular matrix before signs of systemic inflammatory responses are observed. This study further elucidates the early events of endothelial dysfunction during toxic uremia conditions allowing more complete understanding of the molecular events as well as their sequence during progressive renal failure.
authors.affiliation: Department of Biology, University of Patras, Patras, Achaia, Greece.
authors.forename: Kalliopi
authors.initials: K
authors.lastname: Zafeiropoulou
authors.forename: Theodora
authors.initials: T
authors.lastname: Bita
authors.forename: Apostolos
authors.initials: A
authors.lastname: Polykratis
authors.forename: Stella
authors.initials: S
authors.lastname: Karabina
authors.forename: John
authors.initials: J
authors.lastname: Vlachojannis
authors.forename: Panagiotis
authors.initials: P
authors.lastname: Katsoris
mesh_terms: D002465:Cell Movement; D049109:Cell Proliferation; D042783:Endothelial Cells / Q000166:cytology*; D004730:Endothelium, Vascular / Q000473:pathology; D005109:Extracellular Matrix / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D007249:Inflammation; D007676:Kidney Failure, Chronic / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D006435:Renal Dialysis / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D014118:Toxins, Biological / Q000737:chemistry*; D014511:Uremia / Q000097:blood*; D014945:Wound Healing
chemical_list: D014118:Toxins, Biological