title: The C-terminal extension of chloroplast 2-Cys peroxiredoxin is critical for interaction with ATP.
abstract: 2-Cys peroxiredoxins (2-Cys Prxs) are ubiquitous enzymes that have been implicated in peroxide-mediated signaling of markedly different processes, such as cancer and photosynthesis. A highly conserved C-terminal extension of eukaryotic homologues modulates both the overoxidation of cysteines and the formation of oligomers. Here, we reveal that the plant counterpart regulates the self-polymerization of 2-Cys Prx triggered by ATP and Mg(2+). This feature is of particular importance under oxidative stress because the interaction of ATP with 2-Cys Prx rapidly integrates nonredox chemistry of signaling pathways into a network hub governed by multiple redox transformations at cysteine residues.
authors.affiliation: Instituto Leloir, IIBBA-CONICET, Depto. Química Biológica-FCEN-UBA, Patricias Argentinas 435, C1405BWE Buenos Aires, Argentina.
authors.forename: Diego
authors.initials: D
authors.lastname: Ferrero
authors.forename: Martin
authors.initials: M
authors.lastname: Aran
authors.forename: Laura
authors.initials: L
authors.lastname: Rimmaudo
authors.forename: Ricardo A
authors.initials: RA
authors.lastname: Wolosiuk
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry / Q000378:metabolism*; D001665:Binding Sites; D002736:Chloroplasts / Q000737:chemistry*; D003545:Cysteine / Q000235:genetics* / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D054464:Peroxiredoxins / Q000737:chemistry* / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry* / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D010940:Plant Proteins; D000255:Adenosine Triphosphate; D054464:Peroxiredoxins; D003545:Cysteine