title: [In vitro study on gastrointestinal absorption of FITC labeled pilose antler protein extraction].
abstract: An in vitro detection method of the gastrointestinal absorption of Pilose Antler protein was established for mixed protein activity. Five bands of protein with molecular weight of 17.8-160 kD derived from the Pilose Antler were extracted and sufficiently labeled with FITC (FITC-PE). The stability and variation of FITC-PE in gastrointestinal circumstances were detected by native polyacrylamide gel electrophoresis and confocal laser scanning microscope. Results showed that the main component of FITC-PE kept invariant after being reacted with artificial gastric fluid and artificial intestinal fluid. The fluorescence signal was detected 20 min after administration in the valgus intestinal purse experiment, and three kinds of protein, with molecular weight of 45, 25, and 17.8 kD, were detected in the mixture of absorbent protein. The research laid the foundation for the further in vivo study of Pilose Antler protein. Meanwhile, it would be an in vitro screening method for the absorption, distribution and metabolism of mixed protein from traditional Chinese medicine.
authors.affiliation: Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.forename: Jian-Jiang
authors.initials: JJ
authors.lastname: Hu
authors.forename: Qiu-Li
authors.initials: QL
authors.lastname: Zhou
authors.forename: Xin-Yue
authors.initials: XY
authors.lastname: Wang
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D000999:Antlers / Q000737:chemistry*; D003670:Deer; D016650:Fluorescein-5-isothiocyanate; D005753:Gastric Mucosa / Q000378:metabolism; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D008419:Materia Medica / Q000737:chemistry / Q000302:isolation & purification / Q000493:pharmacokinetics*; D018613:Microscopy, Confocal; D008970:Molecular Weight; D063347:Native Polyacrylamide Gel Electrophoresis; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000493:pharmacokinetics*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D008419:Materia Medica; D011506:Proteins; D016650:Fluorescein-5-isothiocyanate