title: A comparison of two approaches to extracting Cryptosporidium DNA from human stools as measured by a real-time PCR assay.
abstract: Direct extraction of Cryptosporidium DNA from 46 stools by bead-beating, guanidine thiocyanate and silica purification provided slightly lower PCR positivity (93.5% vs. 100%) and higher threshold cycle values (mean 34.93 vs. 28.03; P=0.00) than spin-column extraction from boiled, semi-purified oocyst suspensions. However, direct extraction is cheaper, and amenable to automation.
authors.affiliation: UK Cryptosporidium Reference Unit, Public Health Wales Microbiology, Singleton Hospital, Swansea, SA2 8QA, United Kingdom.
authors.forename: K
authors.initials: K
authors.lastname: Elwin
authors.forename: G
authors.initials: G
authors.lastname: Robinson
authors.forename: S J
authors.initials: SJ
authors.lastname: Hadfield
authors.forename: H V
authors.initials: HV
authors.lastname: Fairclough
authors.forename: M
authors.initials: M
authors.lastname: Iturriza-Gómara
authors.forename: R M
authors.initials: RM
authors.lastname: Chalmers
mesh_terms: D019411:Clinical Laboratory Techniques / Q000379:methods*; D003457:Cryptosporidiosis / Q000175:diagnosis* / Q000469:parasitology; D003458:Cryptosporidium / Q000235:genetics / Q000302:isolation & purification*; D016054:DNA, Protozoan / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology*; D006801:Humans; D010274:Parasitology / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D016054:DNA, Protozoan