title: The mucin MUC16 (CA125) binds to NK cells and monocytes from peripheral blood of women with healthy pregnancy and preeclampsia.
abstract: OBJECTIVE
  MUC16 (CA125) released from ovarian tumors binds to NK cells and monocytes via the inhibitory receptor Siglec-9. Here, we investigate whether MUC16 also binds to circulating immune cells during pregnancy and in women with preeclampsia.
  
  
  METHODS
  MUC16 binding was monitored by flow cytometry and immunoprecipitation, and RT-PCR was used to monitor indigenous expression in immune cells. Serum CA125 levels were measured by a clinical assay.
  
  
  RESULTS
  MUC16 was equally distributed on Siglec-9(pos) CD16(pos)/CD56(dim) and CD16(neg)/CD56(br) NK cells in the healthy pregnant and preeclampsia groups. While serum CA125 levels and number of NK and monocytes were similar, increased binding of MUC16 was observed on these immune cells in the preeclampsia cohort as compared to the healthy pregnant samples.
  
  
  CONCLUSIONS
  MUC16 binding to NK cells and monocytes likely contributes to tolerance of the fetal allograft from maternal responses and may also serve as a novel biomarker for preeclampsia.
authors.affiliation: Department of Obstetrics and Gynecology, University of Wisconsin-Madison, Madison, WI 53792, USA.
authors.forename: Chanel
authors.initials: C
authors.lastname: Tyler
authors.forename: Arvinder
authors.initials: A
authors.lastname: Kapur
authors.forename: Mildred
authors.initials: M
authors.lastname: Felder
authors.forename: Jennifer A
authors.initials: JA
authors.lastname: Belisle
authors.forename: Christine
authors.initials: C
authors.lastname: Trautman
authors.forename: Jennifer A A
authors.initials: JA
authors.lastname: Gubbels
authors.forename: Joseph P
authors.initials: JP
authors.lastname: Connor
authors.forename: Manish S
authors.initials: MS
authors.lastname: Patankar
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000378:metabolism; D015415:Biomarkers / Q000097:blood; D018394:CA-125 Antigen / Q000097:blood*; D015331:Cohort Studies; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007694:Killer Cells, Natural / Q000378:metabolism*; D037102:Lectins / Q000378:metabolism; D008565:Membrane Proteins / Q000097:blood*; D009000:Monocytes / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000097:blood*; D011247:Pregnancy / Q000097:blood*; D011485:Protein Binding; D063265:Sialic Acid Binding Immunoglobulin-like Lectins
chemical_list: D015703:Antigens, CD; D015415:Biomarkers; D018394:CA-125 Antigen; D037102:Lectins; C451759:MUC16 protein, human; D008565:Membrane Proteins; C412227:SIGLEC9 protein, human; D063265:Sialic Acid Binding Immunoglobulin-like Lectins