title: Effect of hypobaric hypoxia on the P2X receptors of pyramidal cells in the immature rat hippocampus CA1 sub-field.
abstract: OBJECTIVE
  This study was designed to evaluate the effect of hypobaric hypoxia (HH) on the function and expression of P2X receptors in rat hippocampus CA1 pyramidal cells.
  
  
  METHODS
  The functional changes of P2X receptors were investigated through the cell HH model and the expressional alterations of P2X receptors were observed through the animal HH model.
  
  
  METHODS
  P2X receptors mediated currents were recorded from the freshly dissociated CA1 pyramidal cells of 7-day-old SD rats by whole cell patch clamp recording. The expression and distribution of P2X receptors were observed through immunohistochemistry and western blot at HH 3-day and 7-day.
  
  
  RESULTS
  In acute HH conditions, the amplitudes of ATP evoked peak currents were decreased compared to control. The immunohistochemistry and western blot results reflected there was no change in P2X receptors expression after 3 days HH injury, while P2X receptors expression was up-regulated in response to 7 days HH injury.
  
  
  CONCLUSIONS
  These findings supported the possibility that the function of P2X receptors was sensitive to HH damage and long-term function decrease should result in the expression increase of P2X receptors.
authors.affiliation: Department of Neurobiology, College of Basic Medical Sciences, Chongqing Key Laboratory of Neurobiology, Third Military Medical University, Chongqing, PR China.
authors.forename: Yan-Dong
authors.initials: YD
authors.lastname: Zhao
authors.forename: Sai-Yu
authors.initials: SY
authors.lastname: Cheng
authors.forename: Shan
authors.initials: S
authors.lastname: Ou
authors.forename: Zhi
authors.initials: Z
authors.lastname: Xiao
authors.forename: Wen-Juan
authors.initials: WJ
authors.lastname: He
authors.lastname: Jian-Cui
authors.forename: Huai-Zhen
authors.initials: HZ
authors.lastname: Ruan
mesh_terms: D000531:Altitude; D000818:Animals; D001783:Blood Flow Velocity; D056547:CA1 Region, Hippocampal / Q000098:blood supply / Q000378:metabolism* / Q000503:physiopathology; D006931:Hyperbaric Oxygenation; D002534:Hypoxia, Brain / Q000378:metabolism* / Q000503:physiopathology; D007150:Immunohistochemistry; D017966:Pyramidal Cells / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D058486:Receptors, Purinergic P2X7 / Q000378:metabolism*
chemical_list: C546470:P2rx7 protein, rat; D058486:Receptors, Purinergic P2X7