title: Plasminogen activator inhibitor-1 and angiotensin converting enzyme gene polymorphisms in Turkish asthmatic children.
abstract: BACKGROUND
  Polymorphisms of plasminogen activator inhibitor-1 (PAI-1) and angiotensin-converting enzyme (ACE) genes have been implicated in susceptibility to asthma. In this study, we aimed to investigate whether there was any association between childhood asthma and polymorphisms of the PAI-1 and ACE genes.
  
  
  METHODS
  Two hundred and three Turkish children aged 5-15 years, including 102 asthmatic patients and 101 healthy control subjects were included in this study. The asthma group was divided into two groups as follows: Group I: Asthmatic children with positive family history for atopy (n=53), Group II: Asthmatic children without any family history for atopy (n=49). One hundred and twenty-eight atopic family members were also included in the study. The insertion/deletion (I/D) polymorphism of the ACE and PAI-1 4G/5G gene polymorphisms was carried out by polymerase chain reaction.
  
  
  RESULTS
  The prevalence of the PAI-1 4G allele was significantly greater in asthmatic children compared to control group (p<0.05, OR: 1.64 (1.11-2.43)) but there was no significant relation between ACE I/D genotypes and childhood asthma. No significant difference was detected between Groups I and II in terms of these ACE and PAI-1 genotypes and allele frequencies. No significant relationship was found between both gene polymorphisms and total serum IgE and skin prick test results.
  
  
  CONCLUSIONS
  It has been established that PAI-1 4G allele may be a genetic risk factor for childhood asthma but ACE gene I/D polymorphisms do not play a role in the development of asthma in the sample of Turkish children.
authors.affiliation: Dokuz Eylul University, Faculty of Medicine, Department of Medical Genetics, Balçova, İzmir, Turkey.
authors.forename: E
authors.initials: E
authors.lastname: Bora
authors.affiliation: Dokuz Eylul University, Department of Pediatrics, Division of Pediatric Genetics, Balçova, İzmir, Turkey.
authors.forename: R
authors.initials: R
authors.lastname: Soylar
authors.affiliation: Dokuz Eylul University, Faculty of Medicine, Department of Pediatrics, Division of Pediatric Allergy, Balçova, İzmir, Turkey. Electronic address: ztarikan@hotmail.com.
authors.forename: Z
authors.initials: Z
authors.lastname: Arıkan-Ayyıldız
authors.affiliation: Dokuz Eylul University, Faculty of Medicine, Department of Pediatrics, Division of Pediatric Allergy, Balçova, İzmir, Turkey.
authors.forename: N
authors.initials: N
authors.lastname: Uzuner
authors.affiliation: Dokuz Eylul University, Department of Pediatrics, Division of Pediatric Genetics, Balçova, İzmir, Turkey.
authors.forename: Ö
authors.initials: Ö
authors.lastname: Giray-Bozkaya
authors.affiliation: Dokuz Eylul University, Department of Pediatrics, Division of Pediatric Genetics, Balçova, İzmir, Turkey.
authors.forename: D
authors.initials: D
authors.lastname: Erçal
authors.affiliation: Dokuz Eylul University, Faculty of Medicine, Department of Pediatrics, Division of Pediatric Allergy, Balçova, İzmir, Turkey.
authors.forename: Ö
authors.initials: Ö
authors.lastname: Karaman
authors.affiliation: Dokuz Eylul University, Faculty of Medicine, Department of Medical Genetics, Balçova, İzmir, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Ülgenalp
mesh_terms: D000293:Adolescent; D001249:Asthma / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D004252:DNA Mutational Analysis; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D008297:Male; D007703:Peptidyl-Dipeptidase A / Q000235:genetics*; D017395:Plasminogen Activator Inhibitor 1 / Q000235:genetics*; D011110:Polymorphism, Genetic; D014421:Turkey
chemical_list: D017395:Plasminogen Activator Inhibitor 1; D007073:Immunoglobulin E; C488034:ACE protein, human; D007703:Peptidyl-Dipeptidase A