title: Microfluidic assay without blocking for rapid HIV screening and confirmation.
abstract: The essential step for HIV spreading limitation is the screening tests. However, there are multiple disadvantages in current screening assays which need further confirmation test. Herein we developed a rapid HIV assay combining screening and confirmation test by using the microfluidic network assay. Meanwhile, the assay is accelerated by bypassing the step of blocking. We call this method as microfluidic assay without blocking (MAWB). Both the limit of detection and reagent incubation time of MAWB are determined by screening of one model protein pair: ovalbumin and its antibody. The assay time is accelerated about 25% while the limit of detection (LOD) is well kept. Formatting the method in for both HIV screening (testing 8 HIV-related samples) and confirmation (assaying 6 kinds of HIV antibodies of each sample) within 30 min was successful. Fast HIV screening and confirmation of 20 plasma samples were also demonstrated by this method. MAWB improved the assay speed while keeping the LOD of conventional ELISA. Meanwhile, both the accuracy and throughput of MAWB were well improved, which made it an excellent candidate for a quick HIV test for both screening and confirmation. Methods like this one will find wide applications in clinical diagnosis and biochemical analysis based on the interactions between pairs of molecules.
authors.affiliation: CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, Beijing, China.
authors.forename: Lusheng
authors.initials: L
authors.lastname: Song
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Wenjun
authors.initials: W
authors.lastname: Wang
authors.forename: Liying
authors.initials: L
authors.lastname: Ma
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.forename: Yanlin
authors.initials: Y
authors.lastname: Hao
authors.forename: Yiming
authors.initials: Y
authors.lastname: Shao
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Xingyu
authors.initials: X
authors.lastname: Jiang
mesh_terms: D000818:Animals; D002021:Buffers; D002417:Cattle; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D006678:HIV / Q000302:isolation & purification*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D057230:Limit of Detection; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D010710:Phosphates / Q000737:chemistry; D011136:Polysorbates / Q000737:chemistry; D013997:Time Factors
chemical_list: D002021:Buffers; D004129:Dimethylpolysiloxanes; D010710:Phosphates; D011136:Polysorbates; C013830:baysilon