title: [Cloning, expression and characterization of gene encoding human stem cell growth factor-alpha and its synergetic effect with rhGM-CSF on proliferation of human umbilical cord mesenchymal stem cells].
abstract: To investigate the effect of hSCGF-alpha on human Umbilical Cord Mesenchymal Stem Cells (hUCMSCs), we obtained hSCGF-alpha using genetic engineering, hSCGF-alpha gene was amplified from hUCMSCs cDNA using two-step PCR and was inserted into pET-28a(+) plasmid vector. Induced by IPTG at 20 degrees Celsius for 24 h, the fusion protein expressed in E. coli BL21 (DE3) was mainly existing in soluble form. The recombinant hSCGF-a was purified using NI-NTA affinity chromatography and the purity was up to 90%. The colony forming test revealed that combined use hSCGF-alpha and rmGM-CSF (recombinant murine GM-colony stimulating factor, rmGM-CSF) had granulocyte/macrophage (GM) promoting effects on murine bone marrow GM progenitor. In addition, the results indicated that hSCGF-alpha and rhGM-CSF had stimulatory effect on hUCMSCs and their synergetic effect was the strongest.
authors.affiliation: Guangdong Provincial Key Laboratory of Bioengineering Medicine, Biomedicine Research and Development Centre of Jinan University, Guangzhou 510632, China.
authors.forename: Xinlei
authors.initials: X
authors.lastname: Peng
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Ma
authors.forename: Jing
authors.initials: J
authors.lastname: Rong
authors.forename: Zhenling
authors.initials: Z
authors.lastname: Zhao
authors.forename: Bo
authors.initials: B
authors.lastname: Han
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Yangfei
authors.initials: Y
authors.lastname: Xiang
authors.forename: Qiuying
authors.initials: Q
authors.lastname: Liu
authors.forename: Yifei
authors.initials: Y
authors.lastname: Wang
authors.forename: Zhe
authors.initials: Z
authors.lastname: Ren
authors.forename: Xiangrong
authors.initials: X
authors.lastname: Zhou
authors.forename: Haijia
authors.initials: H
authors.lastname: Chen
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004357:Drug Synergism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology; D019089:Stem Cell Factor / Q000096:biosynthesis* / Q000235:genetics; D014470:Umbilical Cord / Q000166:cytology*
chemical_list: D011993:Recombinant Fusion Proteins; D019089:Stem Cell Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor