title: Use of irradiated human amnion as a matrix for limbal stem cell culture.
abstract: Several ocular diseases affect the corneal surface; the development of effective technologies for the treatment of corneal lesions has brought about an improvement in the quality of life of affected patients. The aim of this study is to culture and characterize limbal stem cells cultured on gamma ((60)Co) radiosterilized human amnion (RHA). Limbal stem cells were isolated from ten preserved samples of corneal transplant. The cells were cultured since primary culture until expanded cells on RHA and stained with monoclonal antibodies to establish their immunophenotype, after which cytokeratin 12 and Vimentin were positive by immunohistochemistry. The immunophenotype remained constant since primary culture until expanded cells in RHA. The RHA and cells construct were structurally integrated. Immunohistochemistry was cytokeratin 12, Vimentin positive, and cytokeratin 19 negative. In vitro limbal cells maintain a constant epithelial transition immunophenotype in culture up to primary culture until expanded cells on RHA.
authors.affiliation: Instituto Nacional de Rehabilitacion, Calz. México Xochimilco No. 289 Col. Arenal de Guadalupe, C.P. 14389, Mexico, Mexico.
authors.forename: Carlos
authors.initials: C
authors.lastname: Landa-Solís
authors.forename: Leticia
authors.initials: L
authors.lastname: Vázquez-Maya
authors.forename: María Esther
authors.initials: ME
authors.lastname: Martínez-Pardo
authors.forename: Ana M
authors.initials: AM
authors.lastname: Brena-Molina
authors.forename: Erika
authors.initials: E
authors.lastname: Ruvalcaba
authors.forename: Ricardo
authors.initials: R
authors.lastname: Gómez
authors.forename: Clemente
authors.initials: C
authors.lastname: Ibarra
authors.forename: Cristina
authors.initials: C
authors.lastname: Velasquillo
mesh_terms: D000650:Amnion / Q000166:cytology* / Q000528:radiation effects*; D015415:Biomarkers / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002470:Cell Survival / Q000528:radiation effects; D002478:Cells, Cultured; D005720:Gamma Rays; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D016850:Limbus Corneae / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology* / Q000528:radiation effects; D013242:Sterilization; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D015415:Biomarkers