title: Analysis of IgG subclasses (IgG1 and IgG3) to recombinant SAG2A protein from Toxoplasma gondii in sequential serum samples from patients with toxoplasmosis.
abstract: The kinetics of the humoral immune response was evaluated using the recombinant SAG2A protein comparatively to soluble Toxoplasma antigen (STAg) by ELISA in sequential serum samples of patients with toxoplasmosis up to 12 months of illness onset. The follow up of IgM and IgA levels to STAg showed a gradual decrease, with the majority of patients (88%) seropositive for IgM up to 12 months of infection, whereas IgA seropositivity was relatively low (78%) compared to IgM (100%) in the first 3 months of infection. The follow up of IgG and IgG1 antibodies showed a similar increasing profile for both SAG2A and STAg, with slightly higher seropositivity for STAg. The kinetics of IgG3 to STAg was similar to that of IgG1, contrasting with the kinetics of IgG3 to SAG2A that showed high levels up to 6 months of infection, with continuous decreasing over the time. Higher IgG3 seropositivity to SAG2A than STAg was also observed in the initial phases of infection. A higher IgG3/IgG1 ratio for SAG2A than STAg was detected in the first 3 months of infection, with decreasing profile over the time. The associations of IgG3/IgG1 ratio>1.0 with positive IgM or IgA antibodies were predominantly found in the first 3 months of infection, whereas associations of IgG3/IgG1 ratio<1.0 with positive IgM or negative IgA antibodies were mostly observed from 3 to 12 months of infection. In conclusion, our results demonstrate a differential kinetics of IgG3 antibodies to SAG2A and STAg in patients with toxoplasmosis up to 12 months of infection. Also, the IgG3/IgG1 ratio to SAG2A in association with classical serological markers of acute phase could be potential tools to distinguish early acute from convalescent phases of Toxoplasma gondii infection.
authors.affiliation: Laboratory of Immunoparasitology, Institute of Biomedical Sciences, Federal University of Uberlândia, 38400-902 Uberlândia, MG, Brazil.
authors.forename: Silas S
authors.initials: SS
authors.lastname: Santana
authors.forename: Deise A O
authors.initials: DA
authors.lastname: Silva
authors.forename: Letícia D
authors.initials: LD
authors.lastname: Vaz
authors.forename: Carlos P
authors.initials: CP
authors.lastname: Pirovani
authors.forename: Geisa B
authors.initials: GB
authors.lastname: Barros
authors.forename: Elenice M
authors.initials: EM
authors.lastname: Lemos
authors.forename: Reynaldo
authors.initials: R
authors.lastname: Dietze
authors.forename: José R
authors.initials: JR
authors.lastname: Mineo
authors.forename: Jair P
authors.initials: JP
authors.lastname: Cunha-Junior
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000953:Antigens, Protozoan / Q000276:immunology*; D002648:Child; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood* / Q000145:classification / Q000276:immunology*; D007075:Immunoglobulin M / Q000097:blood / Q000276:immunology; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D015800:Protozoan Proteins / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D013997:Time Factors; D014122:Toxoplasma / Q000276:immunology*; D014123:Toxoplasmosis / Q000175:diagnosis* / Q000276:immunology*; D055815:Young Adult
chemical_list: D000953:Antigens, Protozoan; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D015800:Protozoan Proteins; D011994:Recombinant Proteins; C067547:surface antigen P22, Toxoplasma