title: The miR-17/92 cluster is targeted by STAT5 but dispensable for mammary development.
abstract: Genome wide analysis revealed the miR-17/92 cluster as a STAT5 target. This cluster encodes six microRNAs, which predictably target genes that play a role in mammary gland development. In this study, we have deleted the miR-17/92 cluster in mammary stem cells and evaluated in the mouse its function during mammary gland development. Loss of the miR-17/92 cluster did not affect mammary development from prepuberty to lactation. Our studies demonstrated that, while expression of the miR-17/92 cluster is under control of the key mammary transcription factor STAT5, its presence is not required for normal mammary development or lactation.
authors.affiliation: Laboratory of Genetics and Physiology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA. feuermanny@mail.nih.gov
authors.forename: Yonatan
authors.initials: Y
authors.lastname: Feuermann
authors.forename: Gertraud W
authors.initials: GW
authors.lastname: Robinson
authors.forename: Bing-Mei
authors.initials: BM
authors.lastname: Zhu
authors.forename: Keunsoo
authors.initials: K
authors.lastname: Kang
authors.forename: Noa
authors.initials: N
authors.lastname: Raviv
authors.forename: Daisuke
authors.initials: D
authors.lastname: Yamaji
authors.forename: Lothar
authors.initials: L
authors.lastname: Hennighausen
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D047369:Chromatin Immunoprecipitation; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D017353:Gene Deletion; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing; D007774:Lactation / Q000235:genetics; D008321:Mammary Glands, Animal / Q000166:cytology / Q000254:growth & development*; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D005810:Multigene Family / Q000502:physiology*; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics; D050799:STAT5 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013234:Stem Cells
chemical_list: C575895:MIRN17-92 microRNA, mouse; D035683:MicroRNAs; D012333:RNA, Messenger; D050799:STAT5 Transcription Factor