title: [Effect of down-regulation of GAT-1 on analgesic action of oxysophoridine].
abstract: OBJECTIVE
  To study the effect of GABA transporter (GAT-1) on the analgesic action of oxysophoridine (OSR) in the central nervous system of mice.
  
  
  METHODS
  Hot plate test was used to observe and analyze the effect of gamma aminobutyric acid and the inhibitor of GAT-1 (NO-711) on the analgesic action of oxysophoridine. Real time RT-PCR was used to investigate the influence of OSR on the expression of GAT-1 mRNA induced by formalin in spinal cord and brain of mice.
  
  
  RESULTS
  Both GABA (2.0 mg x kg(-1), icv) and NO-711(0.125 mg x kg(-1), icv) enhanced the analgesic action of OSR (32.0 mg x kg(-1), iv) in the hot plate test, and the latencies was markedly increased (P < 0.05, P < 0.01). OSR (500.0 mg x kg(-1), iv) significantly inhibited the expression of GAT-1 mRNA induced by formalin (P < 0.05).
  
  
  CONCLUSIONS
  GAT-1 was involved in the analgesia effect of OSR and the down-regulation of GAT-1 mRNA enhanced the analgesic effect.
authors.affiliation: Department of Pharmacy, People's Hospital of Gansu Province, Gansu 730000, China. gaojx82@163.com
authors.forename: Jinxian
authors.initials: J
authors.lastname: Gao
authors.forename: Zhenhong
authors.initials: Z
authors.lastname: Yi
authors.forename: Guang
authors.initials: G
authors.lastname: Yang
authors.forename: Lin
authors.initials: L
authors.lastname: Yan
authors.forename: Lijun
authors.initials: L
authors.lastname: Tao
authors.forename: Shuansheng
authors.initials: S
authors.lastname: Gao
authors.forename: Qiuyan
authors.initials: Q
authors.lastname: Pan
authors.forename: Yuanxu
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D000700:Analgesics / Q000494:pharmacology*; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D015536:Down-Regulation / Q000187:drug effects*; D005260:Female; D050485:GABA Plasma Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D051379:Mice; D012333:RNA, Messenger / Q000032:analysis; D013116:Spinal Cord / Q000187:drug effects / Q000378:metabolism
chemical_list: D000470:Alkaloids; D000700:Analgesics; D050485:GABA Plasma Membrane Transport Proteins; D012333:RNA, Messenger; C406983:oxysophoridine