title: Arabidopsis vacuolar H+-ATPase (V-ATPase) B subunits are involved in actin cytoskeleton remodeling via binding to, bundling, and stabilizing F-actin.
abstract: Vacuolar H(+)-ATPase (V-ATPase) is a membrane-bound multisubunit enzyme complex composed of at least 14 different subunits. The complex regulates the physiological processes of a cell by controlling the acidic environment, which is necessary for certain activities and the interaction with the actin cytoskeleton through its B and C subunits in both humans and yeast. Arabidopsis V-ATPase has three B subunits (AtVAB1, AtVAB2, and AtVAB3), which share 97.27% sequence identity and have two potential actin-binding sites, indicating that these AtVABs may have crucial functions in actin cytoskeleton remodeling and plant cell development. However, their biochemical functions are poorly understood. In this study, we demonstrated that AtVABs bind to and co-localize with F-actin, bundle F-actin to form higher order structures, and stabilize actin filaments in vitro. In addition, the AtVABs also show different degrees of activities in capping the barbed ends but no nucleating activities, and these activities were not regulated by calcium. The functional similarity and differences of the AtVABs implied that they may play cooperative and distinct roles in Arabidopsis cells.
authors.affiliation: School of life Sciences, Lanzhou University, Lanzhou 730070, China.
authors.forename: Binyun
authors.initials: B
authors.lastname: Ma
authors.forename: Dong
authors.initials: D
authors.lastname: Qian
authors.forename: Qiong
authors.initials: Q
authors.lastname: Nan
authors.forename: Chang
authors.initials: C
authors.lastname: Tan
authors.forename: Lizhe
authors.initials: L
authors.lastname: An
authors.forename: Yun
authors.initials: Y
authors.lastname: Xiang
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D003599:Cytoskeleton / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D055550:Protein Stability; D012441:Saccharomyces cerevisiae; D025262:Vacuolar Proton-Translocating ATPases / Q000235:genetics / Q000378:metabolism*
chemical_list: D000199:Actins; D029681:Arabidopsis Proteins; D025262:Vacuolar Proton-Translocating ATPases