title: Fluoroquinolone-resistant extraintestinal Escherichia coli clinical isolates representing the O15:K52:H1 clonal group from humans and dogs in Australia.
abstract: Antimicrobial-resistant extraintestinal pathogenic Escherichia coli (ExPEC) impact both human and veterinary medicine. One ExPEC clonal group that has become increasingly multidrug-resistant is serotype O15:K52:H1. Accordingly, we sought O15:K52:H1 strains among fluoroquinolone-resistant (FQ(r)) E. coli clinical isolates from humans (n=582) and dogs (n=120) in Australia. The phylogenetic group D isolates (267/702; 38%) were screened for O15:K52:H1-specific single-nucleotide polymorphisms (SNPs) in fumC and the O15 rfb variant. The 34 so-identified O15:K52:H1 isolates (33 human, 1 canine) underwent antimicrobial susceptibility profiling, virulence genotyping, and macrorestriction profiling. Although susceptibility profiles varied, the 34 isolates were closely related by pulsed-field gel electrophoresis and exhibited typical O15:K52:H1-associated virulence profiles (complete pap operon, F16 papA allele, papG allele II, iha, fimH, sat, fyuA, iutA, kpsMII, ompT). The canine isolate closely resembled human isolates. Thus, O15:K52:H1 strains contribute to the FQ(r) ExPEC population in Australia and may potentially be transferred between humans and dogs.
authors.affiliation: School of Veterinary Science, The University of Queensland, Gatton, Queensland, Australia. j.platell@uq.edu.au
authors.forename: Joanne L
authors.initials: JL
authors.lastname: Platell
authors.forename: Rowland N
authors.initials: RN
authors.lastname: Cobbold
authors.forename: James R
authors.initials: JR
authors.lastname: Johnson
authors.forename: Connie R
authors.initials: CR
authors.lastname: Clabots
authors.forename: Darren J
authors.initials: DJ
authors.lastname: Trott
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001315:Australia; D015373:Bacterial Typing Techniques; D002999:Clone Cells; D004283:Dog Diseases / Q000188:drug therapy / Q000382:microbiology*; D004285:Dogs; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000188:drug therapy / Q000382:microbiology* / Q000662:veterinary*; D005260:Female; D024841:Fluoroquinolones / Q000494:pharmacology*; D005798:Genes, Bacterial; D005838:Genotype; D006801:Humans; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide; D014774:Virulence
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones