title: Autophagy favors Brucella melitensis survival in infected macrophages.
abstract: This study investigated the role of autophagy in the survival of the invasive bacterium Brucella melitensis strain 16M in murine macrophages. Here, Brucella melitensis 16M was found to trigger autophagosome formation, enhance autophagy flux and increase the expression level of the autophagy marker protein LC3-II. When autophagy was pharmacologically inhibited by 3-methyladenine (3-MA), Brucella replication efficiency was significantly decreased (p < 0.05). These results suggest that autophagy favors Brucella melitensis 16M survival in murine macrophages.
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi, 832003, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Guo
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Chuangfu
authors.initials: C
authors.lastname: Chen
authors.forename: Shengwei
authors.initials: S
authors.lastname: Hu
authors.forename: Yuanzhi
authors.initials: Y
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Qiao
authors.forename: Yan
authors.initials: Y
authors.lastname: Ren
authors.forename: Ke
authors.initials: K
authors.lastname: Zhang
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.forename: Guoqing
authors.initials: G
authors.lastname: Du
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D001343:Autophagy / Q000187:drug effects*; D017347:Brucella melitensis / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification*; D002006:Brucellosis / Q000378:metabolism / Q000382:microbiology; D002460:Cell Line; D008264:Macrophages / Q000378:metabolism / Q000382:microbiology; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000378:metabolism
chemical_list: C480860:Map1lc3b protein, mouse; D008869:Microtubule-Associated Proteins; C025946:3-methyladenine; D000225:Adenine