title: Acadl-SNP based genotyping assay for long-chain acyl-CoA dehydrogenase deficient mice.
abstract: The long-chain acyl-CoA dehydrogenase (LCAD) (Acadl=gene; LCAD=protein) deficient mouse model has been important in evaluating the role of mitochondrial fatty acid oxidation of long-chain fatty acids in metabolic disorders. The insertion vector-based gene targeting strategy used to generate this model has made it difficult to distinguish homozygous and heterozygous genotypes containing targeted Acadl alleles in LCAD-deficient mice. Herein, we describe the design and validation of Acadl SNP genotyping methods capable of distinguishing between heterozygous and homozygous LCAD-deficient mice. The Acadl SNP genotyping assays are effective at allelic discrimination of both C57BL/6 and 129 mouse strain-based Acadl alleles under conditions including, both low purity and quantity genomic DNA templates. This makes the method practical and provides the necessary tools for genotyping the LCAD-deficient mouse model.
authors.affiliation: Metabolic Signaling and Disease Program, Diabetes and Obesity Research Center, Sanford-Burnham Medical Research Institute at Lake Nona, Orlando, FL 32827, USA.
authors.forename: Rita J
authors.initials: RJ
authors.lastname: Luther
authors.forename: Alvin J O
authors.initials: AJ
authors.lastname: Almodovar
authors.forename: Russell
authors.initials: R
authors.lastname: Fullerton
authors.forename: Philip A
authors.initials: PA
authors.lastname: Wood
mesh_terms: D044942:Acyl-CoA Dehydrogenase, Long-Chain / Q000172:deficiency* / Q000235:genetics*; D000483:Alleles; D000818:Animals; D001483:Base Sequence; D004195:Disease Models, Animal; D005227:Fatty Acids / Q000235:genetics / Q000378:metabolism; D005838:Genotype; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide
chemical_list: D005227:Fatty Acids; D044942:Acyl-CoA Dehydrogenase, Long-Chain