title: High enzymatic activity preservation with carbon nanotubes incorporated in urease-lipid hybrid Langmuir-Blodgett films.
abstract: The search for optimized architectures, such as thin films, for the production of biosensors has been challenged in recent decades, and thus, the understanding of molecular interactions that occur at interfaces is essential to improve the construction of nanostructured devices. In this study, we investigated the possibility of using carbon nanotubes in hybrid Langmuir-Blodgett (LB) films of lipids and urease to improve the catalytic performance of the immobilized enzyme. The molecular interactions were first investigated at the air-water interface with the enzyme adsorbed from the aqueous subphase onto Langmuir monolayers of dimyristoylphosphatidic acid (DMPA). The transfer to solid supports as LB films and the subsequent incorporation of carbon nanotubes in the hybrid film permitted us to evaluate how these nanomaterials changed the physical properties of the ultrathin film. Colorimetric measurments indicated that the presence of nanotubes preserved and enhanced the enzyme activity of the film, even after 1 month. These results show that the use of such hybrid films is promising for the development of biosensors with an optimized performance.
authors.affiliation: Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de São Paulo (UNIFESP), Diadema, São Paulo (SP) 09972-270, Brazil. lcaseli@unifesp.br
authors.forename: Luciano
authors.initials: L
authors.lastname: Caseli
authors.forename: José Roberto
authors.initials: JR
authors.lastname: Siqueira
mesh_terms: D015374:Biosensing Techniques; D004800:Enzymes, Immobilized / Q000378:metabolism; D020404:Glycerophospholipids / Q000737:chemistry; D007700:Kinetics; D008051:Lipid Bilayers / Q000737:chemistry; D037742:Nanotubes, Carbon / Q000737:chemistry*; D014510:Urease / Q000378:metabolism
chemical_list: D004800:Enzymes, Immobilized; D020404:Glycerophospholipids; D008051:Lipid Bilayers; D037742:Nanotubes, Carbon; C025602:dimyristoylphosphatidic acid; D014510:Urease