title: Effects of LLLT in combination with bisphosphonate on bone healing in critical size defects: a histological and histometric study in rat calvaria.
abstract: The purpose of this study was to analyze histologically the effect of low-level laser therapy (LLLT) in combination with bisphosphonate on bone healing in surgically created critical size defects (CSD) in rat calvaria. One hundred Wistar female rats sham operated (sham) and ovariectomized (Ovx) were maintained untreated for 1 month to allow for the development of osteopenia in the Ovx animals. A CSD was made in the calvarium of each rat, and the animals were divided into five groups according to following treatments: (1) sham rats (control), (2) Ovx rats, (3) Ovx rats treated with LLLT, (4) Ovx rats treated with bisphosphonate, and (5) Ovx rats treated with bisphosphonate and LLLT. Groups 4 and 5 were irrigated with 1 ml of bisphosphonate, and groups 3 and 5 were submitted to LLLT (GaAlAs), 660 nm, 24 J, and 0.4285 W/cm(2) on the CSD. Ten animals of each treatment were killed at 30 and 60 days. Histomorphometric assessments, using image analysis software, and histological analyses were performed. No defect was completely regenerated with the bone. Histometrically, it can be observed that groups 3 (37.49 ± 1.94%, 43.11 ± 2.39%) and 5 (35.05 ± 1.57%, 41.07 ± 1.89%) showed a significant bone neoformation when compared to groups 1 (16.81 ± 1.57%, 27.54 ± 1.49%), 2 (11.68 ± 0.98%, 22.51 ± 1.05%), and 4 (14.62 ± 1.70%, 25.67 ± 1.41%) in all experimental periods (P < 0.05). It was possible to conclude that the LLLT associated or not with bisphosphonate treatment was effective for stimulating bone formation in CSD in the calvaria of rats submitted to ovariectomy.
authors.affiliation: GEPLO-Group of Study and Research on Laser in Dentistry, Department of Surgery and Integrated Clinic-Division of Periodontics, São Paulo State University (UNESP)-UNESP, Araçatuba, São Paulo, Brazil. vg.garcia@uol.com.br
authors.forename: Valdir Gouveia
authors.initials: VG
authors.lastname: Garcia
authors.forename: Juliana Mendonça
authors.initials: JM
authors.lastname: da Conceição
authors.forename: Leandro Araújo
authors.initials: LA
authors.lastname: Fernandes
authors.forename: Juliano Milanezi
authors.initials: JM
authors.lastname: de Almeida
authors.forename: Maria José Hitomi
authors.initials: MJ
authors.lastname: Nagata
authors.forename: Alvaro Francisco
authors.initials: AF
authors.lastname: Bosco
authors.forename: Leticia Helena
authors.initials: LH
authors.lastname: Theodoro
mesh_terms: D000818:Animals; D050071:Bone Density Conservation Agents / Q000494:pharmacology; D001851:Bone Diseases, Metabolic / Q000601:surgery; D001861:Bone Regeneration / Q000187:drug effects* / Q000528:radiation effects*; D003627:Data Interpretation, Statistical; D004164:Diphosphonates / Q000494:pharmacology*; D005260:Female; D007091:Image Processing, Computer-Assisted; D028022:Low-Level Light Therapy / Q000379:methods*; D010052:Ovariectomy; D051381:Rats; D017208:Rats, Wistar; D012886:Skull / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects* / Q000601:surgery
chemical_list: D050071:Bone Density Conservation Agents; D004164:Diphosphonates