title: Biodegradation of bensulphuron-methyl by a novel Penicillium pinophilum strain, BP-H-02.
abstract: A fungal strain able to rapidly degrade bensulphuron-methyl (BSM), called BP-H-02, was isolated for the first time from soil that had been contaminated with BSM for several years. BP-H-02 can use BSM as the sole carbon and energy source for growth in a mineral salt medium. Based on morphological and internal transcribed spacer (ITS) analysis, BP-H-02 was identified as Penicillium pinophilum. Under optimal conditions (pH 6.5, temperature 30 °C and 200 mg/L VSSinoculum), more than 87% of the initially added BSM (50mg/L) was degraded after 60 h. Metabolites were identified as 2-amino-4,6-dimethoxypyrimidine and 1-(4,6-dimethoxypyrimidin-2-yl) urea by liquid chromatography-mass spectrometry (LC-MS), and a possible degradation pathway was deduced. In a soil bioremediation experiment, inoculation of soil with BP-H-02 promoted the degradation of BSM more effectively than did the control. These results revealed that BP-H-02 can biodegrade bensulphuron-methyl efficiently and could potentially be used to bioremediate sulphonylurea herbicides contamination.
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology, Ministry of Education; and Laboratory of Insect Toxicology, South China Agricultural University, Guangzhou 510642, PR China.
authors.forename: Xingxing
authors.initials: X
authors.lastname: Peng
authors.forename: Jingfei
authors.initials: J
authors.lastname: Huang
authors.forename: Chenglan
authors.initials: C
authors.lastname: Liu
authors.forename: Zhun
authors.initials: Z
authors.lastname: Xiang
authors.forename: Jingyun
authors.initials: J
authors.lastname: Zhou
authors.forename: Guohua
authors.initials: G
authors.lastname: Zhong
mesh_terms: D001673:Biodegradation, Environmental; D002851:Chromatography, High Pressure Liquid; D052918:Environmental Restoration and Remediation; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D010407:Penicillium / Q000254:growth & development / Q000378:metabolism*; D012989:Soil Pollutants / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013453:Sulfonylurea Compounds / Q000737:chemistry*
chemical_list: D007202:Indicators and Reagents; D012989:Soil Pollutants; D013453:Sulfonylurea Compounds; C071475:methyl bensulfuron