title: Rational synthesis of 1,3-diolein by enzymatic esterification.
abstract: t-Butanol was an excellent reaction medium for enzyme-mediated esterification of oleic acid with glycerol for 1,3-diolein preparation which has been proved by our group. Herein, to achieve the rational synthesis of 1,3-diolien, response surface methodology was applied to examine the effects of the significant variables and their reciprocal effects on the product synthesis. Under the optimal conditions (62.4°C, 0.75g Novozym 435, substrate molar ratio (oleic acid/glycerol) 2.4 and 4.8g t-butanol), the diolein yield of 87.4% could be achieved, and the value of 1,3-diolien/diolein was as high as 87.8%, which were quite close to the predicted values. Compared with the results of our previous single factor experiment, although the values of diolein yield and 1,3-diolien/diolein could not be improved markedly, the enzyme dosage and the reaction medium were spared by 25% and 20%, respectively, which was a remarkable improvement of the enzymatic process.
authors.affiliation: Academy of State Administration of Grain, Beijing, PR China. bioduan@gmail.com
authors.forename: Zhang-Qun
authors.initials: ZQ
authors.lastname: Duan
authors.forename: Wei
authors.initials: W
authors.lastname: Du
authors.forename: De-Hua
authors.initials: DH
authors.lastname: Liu
mesh_terms: D000704:Analysis of Variance; D001709:Biotechnology / Q000379:methods*; D004075:Diglycerides / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry; D004800:Enzymes, Immobilized; D004951:Esterification; D005656:Fungal Proteins; D005990:Glycerol / Q000378:metabolism; D008049:Lipase / Q000378:metabolism*; D015233:Models, Statistical; D019301:Oleic Acid / Q000378:metabolism
chemical_list: D004075:Diglycerides; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D019301:Oleic Acid; C093667:Novozyme 435; D008049:Lipase; C089743:1,3-diolein; D005990:Glycerol