title: Isotope-coded, fluorous photoaffinity labeling reagents.
abstract: A pair of isotope-coded, fluorous photoaffinity labeling reagents has been developed and coupled with a peptide. The modified peptides form adducts with methanol upon light illumination, which show characteristic isotope labeling patterns in mass spectra and can be separated from other peptides through fluorous silica.
authors.affiliation: Division of Chemical Biology and Medicinal Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
authors.forename: Zhiquan
authors.initials: Z
authors.lastname: Song
authors.forename: Weigang
authors.initials: W
authors.lastname: Huang
authors.forename: Qisheng
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D002853:Chromatography, Liquid; D003903:Deuterium; D007553:Isotope Labeling; D008027:Light; D013058:Mass Spectrometry; D000432:Methanol / Q000737:chemistry; D010455:Peptides / Q000032:analysis* / Q000737:chemistry; D019920:Photoaffinity Labels / Q000138:chemical synthesis*; D025941:Protein Interaction Mapping / Q000379:methods*; D040901:Proteomics; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D010455:Peptides; D019920:Photoaffinity Labels; D012822:Silicon Dioxide; D003903:Deuterium; D000432:Methanol