title: Protein recognition of hetero-/homoleptic ruthenium(II) tris(bipyridine)s for α-chymotrypsin and cytochrome c.
abstract: We examined the relationship between the structures of hetero-/homoleptic ruthenium(II) tris(bipyridine) metal complexes (Ru(II)(bpy)(3)) and their binding properties for α-chymotrypsin (ChT) and cytochrome c (cyt c). Heteroleptic compound 1a binds to both ChT and cyt c in 1:1 ratio, whereas homoleptic 2 forms 1:2 protein complex with ChT but 1:1 complex with cyt c. These results suggest that the structure of the recognition cavity in Ru(II)(bpy)(3) can be designed for shape complementarity to the targeted proteins. In addition, Ru(II)(bpy)(3) complexes were found to be potent inhibitors of cyt c reduction and to permeate A549 cells.
authors.affiliation: The Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka, Japan.
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Yamaguchi
authors.forename: Nobuo
authors.initials: N
authors.lastname: Kato
authors.forename: Hideki
authors.initials: H
authors.lastname: Azuma
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nagasaki
authors.forename: Junko
authors.initials: J
authors.lastname: Ohkanda
mesh_terms: D015082:2,2'-Dipyridyl / Q000737:chemistry*; D017209:Apoptosis / Q000187:drug effects; D001205:Ascorbic Acid / Q000494:pharmacology; D001665:Binding Sites; D045744:Cell Line, Tumor; D002463:Cell Membrane Permeability; D002918:Chymotrypsin / Q000737:chemistry* / Q000378:metabolism; D056831:Coordination Complexes / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D045304:Cytochromes c / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008958:Models, Molecular; D010084:Oxidation-Reduction; D011199:Potentiometry; D011485:Protein Binding; D012428:Ruthenium / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D056831:Coordination Complexes; D015082:2,2'-Dipyridyl; D012428:Ruthenium; D045304:Cytochromes c; D002918:Chymotrypsin; C117608:alpha-chymotrypsin; D001205:Ascorbic Acid