title: Production of a conjugated fatty acid by Bifidobacterium breve LMC520 from α-linolenic acid: conjugated linolenic acid (CLnA).
abstract: This study was performed to characterize natural CLnA isomer production by Bifidobacterium breve LMC520 of human origin in comparison to conjugated linoleic acid (CLA) production. B. breve LMC520 was found to be highly active in terms of CLnA production, of which the major portion was identified as cis-9,trans-11,cis-15 CLnA isomer by GC-MS and NMR analysis. B. breve LMC520 was incubated for 48 h using MRS medium (containing 0.05% L-cysteine · HCl) under different environmental conditions such as atmosphere, pH, and substrate concentration. The high conversion rate of α-linolenic acid (α-LNA) to CLnA (99%) was retained up to 2 mM α-LNA, and the production was proportionally increased nearly 7-fold with 8 mM by the 6 h of incubation under anaerobic conditions at a wide range of pH values (between 5 and 9). When α-LNA was compared with linoleic acid (LA) as a substrate for isomerization by B. breve LMC520, the conversion of α-LNA was higher than that of LA. These results demonstrated that specific CLnA isomer could be produced through active bacterial conversion at an optimized condition. Because many conjugated octadecatrienoic acids in nature are shown to play many positive roles, the noble isomer found in this study has potential as a functional source.
authors.affiliation: Department of Food and Biotechnology, Korea University, Chungnam 339-700, Korea.
authors.forename: Hui Gyu
authors.initials: HG
authors.lastname: Park
authors.forename: Hyung Taek
authors.initials: HT
authors.lastname: Cho
authors.forename: Myoung-Chong
authors.initials: MC
authors.lastname: Song
authors.forename: Sang Bum
authors.initials: SB
authors.lastname: Kim
authors.forename: Eung Gi
authors.initials: EG
authors.lastname: Kwon
authors.forename: Nag Jin
authors.initials: NJ
authors.lastname: Choi
authors.forename: Young Jun
authors.initials: YJ
authors.lastname: Kim
mesh_terms: D001644:Bifidobacterium / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D006863:Hydrogen-Ion Concentration; D044243:Linoleic Acids, Conjugated / Q000096:biosynthesis*; D009682:Magnetic Resonance Spectroscopy; D017962:alpha-Linolenic Acid / Q000378:metabolism*
chemical_list: D044243:Linoleic Acids, Conjugated; D017962:alpha-Linolenic Acid