title: Production of bacillomycin- and macrolactin-type antibiotics by Bacillus amyloliquefaciens NJN-6 for suppressing soilborne plant pathogens.
abstract: Bacillus amyloliquefaciens  strains have been used as biocontrol agents for the suppression of several soilborne plant pathogens. A clearer understanding of the antagonistic mechanisms of action of these bacteria will facilitate their use in the control of plant diseases. Antagonistic substances were isolated from the fermentation broth of  B. amyloliquefaciens  strain NJN-6 cultures. These compounds were preconcentrated using an XAD-16 column and were purified using reversed-phase high-performance liquid chromatography (RP-HPLC). Fractions were collected from the column and were analyzed, and two homologues of bacillomycin D [molecular weights of 1030 Da (C14) and 1044 Da (C15)] and three homologues of members of the macrolactin family, macrolactin A, 7-O-malonyl macrolactin A, and 7-O-succinyl macrolactin A (molecular weights of 402, 487, and 502 Da, respectively) were identified using HPLC/electrospray ionization mass spectrometry (ESI-MS) analysis. An antagonistic assay showed that bacillomycin D and macrolactin exhibited significant antagonistic effects against  Fusarium oxysporum  and  Ralstonia solanacearum , respectively. A reliable method for the isolation and purification of bacillomycin D and macrolactin from bacterial broth cultures was developed. These data will help elucidate the mechanisms that  B. amyloliquefaciens  NJN-6 uses for the biocontrol of soilborne plant pathogens.
authors.affiliation: Jiangsu Key Laboratory for Organic Solid Waste Utilization, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yuan
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.forename: Nan
authors.initials: N
authors.lastname: Zhang
authors.forename: Raza
authors.initials: R
authors.lastname: Waseem
authors.forename: Qirong
authors.initials: Q
authors.lastname: Shen
authors.forename: Qiwei
authors.initials: Q
authors.lastname: Huang
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides; D001407:Bacillus / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005670:Fusarium / Q000187:drug effects; D018942:Macrolides / Q000378:metabolism* / Q000494:pharmacology; D010455:Peptides / Q000378:metabolism* / Q000494:pharmacology; D010935:Plant Diseases / Q000382:microbiology* / Q000517:prevention & control; D043367:Ralstonia / Q000187:drug effects; D012988:Soil Microbiology; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D018942:Macrolides; D010455:Peptides; C453562:macrolactin A; C027845:bacillomycin D