title: Incidence of temonera, sulphuhydryl variables and cefotaximase genes associated with β-lactamase producing escherichia coli in clinical isolates.
abstract: BACKGROUND
  the occurrence of the different types of Extended spectrum beta Lactamase producing Escherichia coli with the, Sulphurhydryl variable, Temonera and the Cefotaximase have been on the rise
  
  
  OBJECTIVE
  The study was to determine the prevalence of extended spectrum beta lactamase gene resistance across the clinical isolates of hospitalized patients.
  
  
  METHODS
  Three hundred and fifty isolates of Escherichia coli were received from different clinical specimens. The susceptibility profile of the isolates against 10 different antibiotics was examined, the MICs (Minimum Inhibitory Concentration) for ceftazidime were also determined using micro-broth dilution assay. Isolates showing MIC ≥ 6 μg/ml for ceftazidime were screened for ESBL (PCT)phenotypic confirmatory test and subjected to PCR (polymerase chain reaction) to further.
  
  
  RESULTS
  By disk diffusion test, there was resistance to ceftazidime and cefotaxime were 180(51.4%) and 120 (34.2%) respectively. However, all strains were susceptible to imipenem. 250 isolates showed MICs≥ 6 μg/ml for ceftazidime of which 180 (72%) were positive for extended spectrum beta lactamase. The prevalence of Sulphurhydryl variable, Temonera and the Cefotaximase among these isolates were 17.1%, 6.6% and 17%, respectively.
  
  
  CONCLUSIONS
  For the identification of extended spectrum beta lactamase producing isolates it is recommended that clinical laboratories adopt simple test based on Cinical laboratory standard institute recommendation for confirming extended spectrum beta lactamase production in enterobacteriacea species.
authors.affiliation: Department of Medical Microbiology, Federal Medical Centre Yola Adamawa State, Nigeria.
authors.forename: Ibeh Nnana
authors.initials: IN
authors.lastname: Isaiah
authors.forename: Bikwe Thomas
authors.initials: BT
authors.lastname: Nche
authors.forename: Ibeh Georgina
authors.initials: IG
authors.lastname: Nwagu
authors.forename: Ibeh Isaiah
authors.initials: II
authors.lastname: Nwagu