title: Metabolites from the leaf extract of Serjania yucatanensis with trypanocidal activity against Trypanosoma cruzi.
abstract: The bioassay-guided phytochemical investigation of the leaf extract of Serjania yucatanensis, a woody climbing plant endemic to the Yucatan peninsula, led to the identification of a mixture of a triterpene [lup-20(29)-en-3-one] and an oxygenated sesquiterpene (β-caryophyllene oxide), as that responsible for the originally detected trypanocidal activity in the organic crude extract. Results showed that the mixture of lup-20(29)-en-3-one and β-caryophyllene oxide is active against trypomastigotes of Trypanosoma cruzi (IC(50) =80.3 μg/mL) and inhibits the egress of trypomastigotes from infected Vero cells (when tested at 100 μg/mL) without being cytotoxic.
authors.affiliation: Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, Calle 43 No. 130, Colonia Chuburná de Hidalgo, Mérida, Yucatán, 97200, Mexico.
authors.forename: Glendy
authors.initials: G
authors.lastname: Polanco-Hernández
authors.forename: Fabiola
authors.initials: F
authors.lastname: Escalante-Erosa
authors.forename: Karlina
authors.initials: K
authors.lastname: García-Sosa
authors.forename: Manuel J
authors.initials: MJ
authors.lastname: Chan-Bacab
authors.forename: Hernán
authors.initials: H
authors.lastname: Sagua-Franco
authors.forename: Jorge
authors.initials: J
authors.lastname: González
authors.forename: Luis
authors.initials: L
authors.lastname: Osorio-Rodríguez
authors.forename: Luis M
authors.initials: LM
authors.lastname: Peña-Rodríguez
mesh_terms: D000818:Animals; D000981:Antiprotozoal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001681:Biological Assay; D002522:Chlorocebus aethiops; D020128:Inhibitory Concentration 50; D021261:Parasitic Sensitivity Tests; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D029631:Sapindaceae / Q000737:chemistry*; D012717:Sesquiterpenes / Q000032:analysis / Q000494:pharmacology; D014315:Triterpenes / Q000032:analysis / Q000494:pharmacology; D014349:Trypanosoma cruzi / Q000187:drug effects*; D014709:Vero Cells
chemical_list: D000981:Antiprotozoal Agents; D010936:Plant Extracts; D012717:Sesquiterpenes; D014315:Triterpenes