title: Resonance assignments of the 56 kDa chimeric avidin in the biotin-bound and free forms.
abstract: Avidin is a homotetrameric ~56 kDa protein found in chicken egg white. Avidin's ability to bind biotin with a very high affinity has widely been exploited in biotechnological applications. Protein engineering has further diversified avidin's feasibility. ChiAVD(I117Y) is a product of rational protein engineering. It is a hyperthermostable synthetic hybrid of avidin and avidin-related protein 4 (AVR4). In this chimeric protein a 23-residue segment in avidin has been replaced with the corresponding sequence found in AVR4, and a point mutation at subunit interface 1-3 (and 2-4) has been introduced. Here we report the backbone and sidechain resonance assignments of the biotin-bound form of ChiAVD(I117Y) as well as the backbone resonance assignments of the free form.
authors.affiliation: Program in Structural Biology and Biophysics, Institute of Biotechnology, Viikinkaari 1, P.O.Box 65, 00014, Helsinki, Finland.
authors.forename: Helena
authors.initials: H
authors.lastname: Tossavainen
authors.forename: Satu H
authors.initials: SH
authors.lastname: Helppolainen
authors.forename: Juha A E
authors.initials: JA
authors.lastname: Määttä
authors.forename: Tero
authors.initials: T
authors.lastname: Pihlajamaa
authors.forename: Vesa P
authors.initials: VP
authors.lastname: Hytönen
authors.forename: Markku S
authors.initials: MS
authors.lastname: Kulomaa
authors.forename: Perttu
authors.initials: P
authors.lastname: Permi
mesh_terms: D001360:Avidin / Q000737:chemistry* / Q000378:metabolism*; D001710:Biotin / Q000378:metabolism*; D008024:Ligands; D008970:Molecular Weight; D019906:Nuclear Magnetic Resonance, Biomolecular; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D008024:Ligands; D011993:Recombinant Fusion Proteins; D001360:Avidin; D001710:Biotin