title: Tumor accumulation, penetration, and antitumor response of cisplatin-loaded gelatin/poly(acrylic acid) nanoparticles.
abstract: In this report, the cisplatin (CDDP)-loaded gelatin/poly(acrylic acid) (GEL-PAA) nanoparticles with a spherical shape and drug loading content of 24.6% were prepared. In vivo near-infrared fluorescence (NIRF) imaging and ex vivo gamma scintillation counting analyses reveal that CDDP-loaded GEL-PAA nanoparticles have prominent passive tumor-targeting ability and the nontarget nanoparticles can be readily excreted from the body. Further, it is demonstrated that the CDDP-loaded nanoparticles have the ability to penetrate the tumor after their extravasation through the leaky vessels and distribute in a distance of about 20 μm from the vessels at 24 h postinjection. The in vivo antitumor responses reveal that the nanoparticle formulation exhibits significantly superior in vivo antitumor effect than free CDDP by the comparison of tumor volume and the examinations of cell apoptosis and proliferation in tumor tissues through proliferating cell nuclear antigen (PCNA) and terminal deoxynucleotidyl-transferase-mediated nick end labeling (TUNEL) methods.
authors.affiliation: Laboratory of Mesoscopic Chemistry and Department of Polymer Science & Engineering, College of Chemistry & Chemical Engineering, Nanjing University, Nanjing 210093, PR China.
authors.forename: Dan
authors.initials: D
authors.lastname: Ding
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Zhenshu
authors.initials: Z
authors.lastname: Zhu
authors.forename: Rutian
authors.initials: R
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.forename: Baorui
authors.initials: B
authors.lastname: Liu
authors.forename: Xiqun
authors.initials: X
authors.lastname: Jiang
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D019921:Biotinylation; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000494:pharmacology / Q000627:therapeutic use*; D016503:Drug Delivery Systems; D005780:Gelatin / Q000737:chemistry*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009369:Neoplasms / Q000188:drug therapy* / Q000378:metabolism*; D013194:Staining and Labeling; D013667:Technetium; D014018:Tissue Distribution / Q000187:drug effects
chemical_list: D000180:Acrylic Resins; D000970:Antineoplastic Agents; C006903:carbopol 940; D013667:Technetium; D005780:Gelatin; D002945:Cisplatin