title: Isolation and functional characterization of a transcription factor VpNAC1 from Chinese wild Vitis pseudoreticulata.
abstract: NAC (for NAM, ATAF1, 2, and CUC2) family genes encode plant-specific transcription factors that play important roles in plant development regulation and in abiotic and biotic stresses. However, the function of NAC genes in grapevines is not clear. A novel NAC transcription factor, designated as VpNAC1, was isolated from Chinese wild Vitis pseudoreticulata. It belongs to the TERN subgroup and is a nuclear-targeting protein and functions as a transcriptional activator. Moreover, VpNAC1 was induced by the fungus Erysiphe necator and the exogenous hormones, particularly salicylic acid, methyl jasmonate and ethylene. Over-expression of VpNAC1 in tobacco plants enhanced their resistance to Erysiphe cichoracearum and Phytophthora parasitica var. nicotianae Tucker. These results suggest that VpNAC1 acts as a positive regulator in biotic stresses.
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling, 712100 Shaanxi, People's Republic of China. shanhong98@163.com
authors.forename: Ziguo
authors.initials: Z
authors.lastname: Zhu
authors.forename: Jiangli
authors.initials: J
authors.lastname: Shi
authors.forename: Mingyang
authors.initials: M
authors.lastname: He
authors.forename: Jiangling
authors.initials: J
authors.lastname: Cao
authors.forename: Yuejin
authors.initials: Y
authors.lastname: Wang
mesh_terms: D001203:Ascomycota / Q000472:pathogenicity; D002681:China; D003517:Cyclopentanes / Q000378:metabolism; D005030:Ethylenes / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D019913:Nuclear Localization Signals; D054883:Oxylipins / Q000378:metabolism; D010838:Phytophthora / Q000472:pathogenicity; D010940:Plant Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D020156:Salicylic Acid / Q000378:metabolism; D013312:Stress, Physiological; D015534:Trans-Activators / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D027843:Vitis / Q000187:drug effects / Q000201:enzymology* / Q000382:microbiology
chemical_list: D003517:Cyclopentanes; D005030:Ethylenes; D019913:Nuclear Localization Signals; D054883:Oxylipins; D010940:Plant Proteins; D015534:Trans-Activators; C011006:jasmonic acid; C036216:ethylene; D020156:Salicylic Acid