title: Experimental canine model for sentinel lymph node biopsy in the vulva using technetium and patent blue dye.
abstract: OBJECTIVE
  This paper aims to study and define the experimental model of sentinel lymph node biopsy of the vulva in bitches.
  
  
  METHODS
  0.2 ml of 99mTc phytate was injected intradermally, using a fine gauge insulin needle in the anterior commissure of the vulva. Thirty minutes after 99mTc injection, the inguinal mapping was performed using a gamma probe. After this, 0.5 ml of blue dye (bleu patenté V Guerbet 2.5%) was injected in the same place. After 15 minutes, a 3 cm long inguinal incision was made at point maximum uptake followed by careful dissection, guided by visualization of a bluish afferent lymphatic system that points to the sentinel lymph node (SLN).
  
  
  RESULTS
  It was observed that 88% of SLN were identified. It wasn't found a significant difference among the presence or not of sentinel lymph node in the sides, which is an indication of a good consistency. It was observed a high (88%) and significant (χ2=12.89 and p=0.0003) intercession between both methods (blue dye and radiation).
  
  
  CONCLUSIONS
  The experimental model adopted is feasible, becoming advantageous in applying the association of Patent blue and 99mTc.
authors.affiliation: Department of Surgery, Faculty of Medicine, Sao Luis, Brazil.
authors.forename: José Ulcijara
authors.initials: JU
authors.lastname: Aquino
authors.forename: Luiz Gonzaga Porto
authors.initials: LG
authors.lastname: Pinheiro
authors.forename: Paulo Henrique Diógenes
authors.initials: PH
authors.lastname: Vasques
authors.forename: João Ivo Xavier
authors.initials: JI
authors.lastname: Rocha
authors.forename: Diego Alves
authors.initials: DA
authors.lastname: Cruz
authors.forename: Hugo Enrique Orsini
authors.initials: HE
authors.lastname: Beserra
authors.forename: Raissa Vasconcelos
authors.initials: RV
authors.lastname: Cavalcante
mesh_terms: D000818:Animals; D004396:Coloring Agents; D004195:Disease Models, Animal; D004285:Dogs; D005260:Female; D005720:Gamma Rays; D007264:Inguinal Canal / Q000601:surgery; D008198:Lymph Nodes / Q000473:pathology / Q000601:surgery; D019275:Radiopharmaceuticals; D015203:Reproducibility of Results; D012394:Rosaniline Dyes; D021701:Sentinel Lymph Node Biopsy / Q000379:methods*; D013667:Technetium; D013997:Time Factors; D014846:Vulvar Neoplasms / Q000473:pathology* / Q000601:surgery*
chemical_list: D004396:Coloring Agents; D019275:Radiopharmaceuticals; D012394:Rosaniline Dyes; D013667:Technetium