title: Comparison of the principal proteins in bovine, caprine, buffalo, equine and camel milk.
abstract: Proteomic analysis of bovine, caprine, buffalo, equine and camel milk highlighted significant interspecies differences. Camel milk was found to be devoid of β-lactoglobulin, whereas β-lactoglobulin was the major whey protein in bovine, buffalo, caprine, and equine milk. Five different isoforms of κ-casein were found in camel milk, analogous to the micro-heterogeneity observed for bovine κ-casein. Several spots observed in 2D-electrophoretograms of milk of all species could tentatively be identified as polypeptides arising from the enzymatic hydrolysis of caseins. The understanding gained from the proteomic comparison of these milks may be of relevance both in terms of identifying sources of hypoallergenic alternatives to bovine milk and detection of adulteration of milk samples and products.
authors.affiliation: School of Food and Nutritional Sciences, University College Cork, Cork, Ireland.
authors.forename: Katharina
authors.initials: K
authors.lastname: Hinz
authors.forename: Paula M
authors.initials: PM
authors.lastname: O'Connor
authors.forename: Thom
authors.initials: T
authors.lastname: Huppertz
authors.forename: R Paul
authors.initials: RP
authors.lastname: Ross
authors.forename: Alan L
authors.initials: AL
authors.lastname: Kelly
mesh_terms: D000818:Animals; D002020:Buffaloes; D002162:Camelus; D002364:Caseins / Q000032:analysis; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D006041:Goats; D006736:Horses; D007782:Lactoglobulins / Q000032:analysis; D008892:Milk / Q000737:chemistry; D016269:Milk Hypersensitivity / Q000517:prevention & control; D008894:Milk Proteins / Q000032:analysis* / Q000378:metabolism; D059748:Proteolysis; D040901:Proteomics; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D000067816:Whey Proteins
chemical_list: D002364:Caseins; D007782:Lactoglobulins; D008894:Milk Proteins; D000067816:Whey Proteins