title: Evaluation of early regenerative processes in a preclinical pig model of acute kidney injury.
abstract: Renal failure due to ischemic injury is a common denominator of various clinical situations in critically ill patients. This study was designed to characterize the TPSO/Cholesterol synthesis and cell division pathways in response to different levels of ischemia. Porcine kidneys were subjected to either 60 min-warm ischemia (WI) or auto-transplanted after cold storage for 24 h at 4°C (CS), or both conditions (WI+CS), pathway activation and function were evaluated at 3 h, 3 and 7 days after reperfusion. CS combined to WI affects renal functions indicating a high degree of injury. During the first week of reperfusion, renal levels of free and esterified cholesterol, major cellular components, increased in CS group with an attenuated production when WI was associated. CS and WI+CS groups exhibited an elevated expression of cell cycle induction markers such as PCNA and stathmin. TSPO expression was highest in groups with the lowest injury, and correlated with kidney outcome, revealing its potential for diagnosis.
authors.affiliation: INSERM U927, Poitiers, France.
authors.forename: L
authors.initials: L
authors.lastname: Rossard
authors.forename: F
authors.initials: F
authors.lastname: Favreau
authors.forename: J
authors.initials: J
authors.lastname: Demars
authors.forename: R
authors.initials: R
authors.lastname: Robert
authors.forename: C
authors.initials: C
authors.lastname: Nadeau
authors.forename: J
authors.initials: J
authors.lastname: Cau
authors.forename: R
authors.initials: R
authors.lastname: Thuillier
authors.forename: T
authors.initials: T
authors.lastname: Hauet
mesh_terms: D058186:Acute Kidney Injury / Q000378:metabolism / Q000503:physiopathology*; D000818:Animals; D002788:Cholesterol Esters / Q000096:biosynthesis; D003080:Cold Temperature; D015870:Gene Expression; D005919:Glomerular Filtration Rate; D007668:Kidney / Q000098:blood supply / Q000378:metabolism / Q000503:physiopathology*; D016030:Kidney Transplantation; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000378:metabolism; D018079:Receptors, GABA / Q000235:genetics / Q000378:metabolism; D012038:Regeneration; D015427:Reperfusion Injury / Q000378:metabolism / Q000503:physiopathology; D050777:Stathmin / Q000235:genetics / Q000378:metabolism; D013552:Swine
chemical_list: D002788:Cholesterol Esters; D018809:Proliferating Cell Nuclear Antigen; D018079:Receptors, GABA; D050777:Stathmin