title: Benzophenone synthase from Garcinia mangostana L. pericarps.
abstract: The cDNA of a benzophenone synthase (BPS), a type III polyketide synthase (PKS), was cloned and the recombinant protein expressed from the fruit pericarps of Garcinia mangostana L., which contains mainly prenylated xanthones. The obtained GmBPS showed an amino acid sequence identity of 77-78% with other plant BPSs belonging to the same family (Clusiaceae). The recombinant enzyme produced 2,4,6-trihydroxybenzophenone as the predominant product with benzoyl CoA as substrate. It also accepted other substrates, such as other plant PKSs, and used 1-3 molecules of malonyl CoA to form various phloroglucinol-type and polyketide lactone-type compounds. Thus, providing GmBPS with various substrates in vivo might redirect the xanthone biosynthetic pathway.
authors.affiliation: Khon-Kaen University, Khon-Kaen 40002, Thailand. nnatsa@kku.ac.th
authors.forename: Natsajee
authors.initials: N
authors.lastname: Nualkaew
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Morita
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Shimokawa
authors.forename: Keishi
authors.initials: K
authors.lastname: Kinjo
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Kushiro
authors.forename: Wanchai
authors.initials: W
authors.lastname: De-Eknamkul
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Ebizuka
authors.forename: Ikuro
authors.initials: I
authors.lastname: Abe
mesh_terms: D000595:Amino Acid Sequence; D019735:Carbon-Carbon Ligases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics; D029762:Garcinia mangostana / Q000201:enzymology* / Q000235:genetics; D007700:Kinetics; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D013379:Substrate Specificity
chemical_list: D018076:DNA, Complementary; D010940:Plant Proteins; D019735:Carbon-Carbon Ligases; C098887:benzophenone synthase