title: Sublingual administration of Lactobacillus paracasei KW3110 inhibits Th2-dependent allergic responses via upregulation of PD-L2 on dendritic cells.
abstract: Lactic acid bacteria have potential in immunomodulation therapy, but their clinical efficacy and underlying mechanisms are unclear. We aimed to clarify the anti-allergic immune responses induced by intragastric and sublingual administration of heat-killed Lactobacillus paracasei KW3110 and Lactobacillus acidophilus L-92. The KW3110 strain (but not the L-92 strain) enhanced ovalbumin (OVA)-induced expression of CCR-7 and PD-L2 in murine dendritic cells (DCs), and strongly inhibited IL-5 and IL-13 production in vitro in co-cultures with Th2-skewed CD4(+) T cells from DO11.10 transgenic mice. Sublingual administration of low-dose KW3110 (but not L-92) to OVA-sensitized mice selectively suppressed serum IgE production and Th2 cytokine expression in cervical lymph nodes, and significantly improved symptoms after OVA provocation in vivo. KW3110 probably accelerates DC migration into the regional lymph nodes and inhibits Th2 cytokine production through enhanced CCR-7 and PD-L2 expression. Thus, sublingual KW3110 administration may be effective in reducing allergic inflammation.
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery (J2), Graduate School of Medicine, Chiba University, Chiba, Japan. ainamine@faculty.chiba-u.jp
authors.forename: Ayako
authors.initials: A
authors.lastname: Inamine
authors.forename: Daijyu
authors.initials: D
authors.lastname: Sakurai
authors.forename: Shigetoshi
authors.initials: S
authors.lastname: Horiguchi
authors.forename: Syuji
authors.initials: S
authors.lastname: Yonekura
authors.forename: Toyoyuki
authors.initials: T
authors.lastname: Hanazawa
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Hosokawa
authors.forename: Asaka
authors.initials: A
authors.lastname: Matuura-Suzuki
authors.forename: Toshinori
authors.initials: T
authors.lastname: Nakayama
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Okamoto
mesh_terms: D000286:Administration, Sublingual; D000818:Animals; D018926:Anti-Allergic Agents / Q000008:administration & dosage*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002465:Cell Movement; D016207:Cytokines / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D007778:Lactobacillus; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology; D010587:Phagocytosis; D061027:Programmed Cell Death 1 Ligand 2 Protein / Q000096:biosynthesis / Q000276:immunology*; D054400:Receptors, CCR7 / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology*; D015854:Up-Regulation
chemical_list: D018926:Anti-Allergic Agents; C515371:Ccr7 protein, mouse; D016207:Cytokines; C556978:Pdcd1lg2 protein, mouse; D061027:Programmed Cell Death 1 Ligand 2 Protein; D054400:Receptors, CCR7; D007073:Immunoglobulin E; D010047:Ovalbumin