title: In vitro upregulation of erythrocytes glucose uptake by Rhaphnus sativa extract in diabetic patients.
abstract: In diabetes, both the increase in the oxidative stress and the decrease in the antioxidant defense may elevate the susceptibility of diabetic patients to many pathological complications. So, the aim of the present study was to investigate the effect of superoxide dismutase (SOD) like activity protein, partially purified from radish (Rhaphnus sativa) on uptake of glucose in vitro by erythrocytes of diabetic patients. In hyperglycemic patients, erythrocytes malondialdehyde level was highly significantly increased (P < 0.0001) than that of the control. However, the erythrocytes glutathione content and glutathione reductase activity, were both highly significantly decreased (P < 0.0001) compared to that corresponding control values. The glucose uptake by erythrocytes of diabetic patients was highly significantly decreased (P < 0.0001) with increasing hyperglycemia, while it was highly significantly elevated (p < 0.0001) after addition of the partially purified SOD like activity protein. On the other hand, the malondialdehyde concentration was highly significantly reduced (p < 0.001) on adding the partially purified protein. It thus can be concluded that, an appropriate support for enhancing antioxidant supply, such as SOD like activity protein from natural sources, may help control blood glucose level and may prevent clinical complications of diabetes.
authors.affiliation: Biochemistry Division, Damietta Faculty of Science, Mansoura University, Central Area, New Damietta, Damietta, Egypt. salem_habib@yahoo.com
authors.forename: Salem A
authors.initials: SA
authors.lastname: Habib
authors.forename: Eman M
authors.initials: EM
authors.lastname: Othman
mesh_terms: D000328:Adult; D001786:Blood Glucose / Q000187:drug effects; D003920:Diabetes Mellitus / Q000378:metabolism*; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism*; D005260:Female; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D010936:Plant Extracts; D013482:Superoxide Dismutase; D005947:Glucose