title: Determination of the genotoxic effects of Convolvulus arvensis extracts on corn (Zea mays L.) seeds.
abstract: In this research, the methanolic extracts of Convolvulus arvensis were tested for genotoxic and inhibitor activity on the total soluble protein content and the genomic template stability against corn Zea mays L. seed. The methanol extracts of leaf, stem and root of C. arvensis were diluted to 50, 75 and 100 μl concentrations and applied to corn seed. The total soluble protein and genomic template stability results were compared with the control. The results showed that especially 100 μl extracts of diluted leaf, stem and root had a strong inhibitory activity on the genomic template stability. The changes occurred in random amplification of polymorphic DNA (RAPD) profiles of C. arvensis extract treatment included variation in band intensity, loss of bands and appearance of new bands compared with control. Also, the results obtained from this study revealed that the increase in the concentrations of C. arvensis extract increased the total soluble protein content in maize. The results suggested that RAPD analysis and total protein analysis could be applied as a suitable biomarker assay for the detection of genotoxic effects of plant allelochemicals.
authors.affiliation: Department of Biology, Atatürk University, Erzurum, Turkey.
authors.forename: Serap
authors.initials: S
authors.lastname: Sunar
authors.forename: Nalan
authors.initials: N
authors.lastname: Yildirim
authors.forename: Ozkan
authors.initials: O
authors.lastname: Aksakal
authors.forename: Guleray
authors.initials: G
authors.lastname: Agar
mesh_terms: D031254:Convolvulus / Q000737:chemistry*; D018744:DNA, Plant / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D042822:Genomic Instability / Q000187:drug effects; D000432:Methanol; D009152:Mutagenicity Tests; D009153:Mutagens / Q000737:chemistry / Q000633:toxicity*; D010936:Plant Extracts / Q000737:chemistry / Q000633:toxicity*; D018515:Plant Leaves / Q000737:chemistry; D010940:Plant Proteins / Q000032:analysis; D018517:Plant Roots / Q000737:chemistry; D019105:Random Amplified Polymorphic DNA Technique; D012639:Seeds / Q000737:chemistry / Q000187:drug effects*; D003313:Zea mays / Q000737:chemistry / Q000187:drug effects*
chemical_list: D018744:DNA, Plant; D009153:Mutagens; D010936:Plant Extracts; D010940:Plant Proteins; D000432:Methanol