title: Synthesis of a fluorescently labeled compound for the detection of arsenic-induced apoptotic HL60 cells.
abstract: Arsenic compounds have shown medical usefulness since they proved to be effective in causing complete remission of acute promyelocytic leukemia. In this work we obtained a fluorescently labeled arsenic compound that can be used with current fluorescence techniques for basic and applied research, focused on arsenic-induced apoptosis studies. This compound is an arsanilic acid bearing a covalently linked FITC that was chemically synthesized and characterized by fluorescence, UV-Vis, mass and FTIR spectrometry. In addition, we assessed its apoptotic activity as well as its fluorescent labeling properties in HL60 cell line as a leukemia cell model through flow cytometry. We obtained a compound with a 1:1 FITC:arsenic ratio and a 595 m/z, confirming its structure by FTIR. This compound proved to be useful at inducing apoptosis in the leukemia cell model and labeling this apoptotic cell population, in such a way that the highest FITC fluorescence correlated with the highest arsenic amount.
authors.affiliation: Laboratorio de Biomembranas, Departamento de Ciencia y Tecnologia, Universidad Nacional de Quilmes, Roque Saenz Pena 352, Bernal, B1876BXD, Buenos Aires, Argentina.
authors.forename: A Lis
authors.initials: AL
authors.lastname: Femia
authors.forename: C Facundo
authors.initials: CF
authors.lastname: Temprana
authors.forename: M Silvia
authors.initials: MS
authors.lastname: Amor
authors.forename: Mariano
authors.initials: M
authors.lastname: Grasselli
authors.forename: Silvia Del V
authors.initials: Sdel V
authors.lastname: Alonso
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001147:Arsanilic Acid / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D002469:Cell Separation / Q000379:methods*; D004354:Drug Screening Assays, Antitumor; D005434:Flow Cytometry; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000032:analysis* / Q000138:chemical synthesis* / Q000737:chemistry; D018922:HL-60 Cells; D006801:Humans; D017879:Isothiocyanates / Q000737:chemistry; D015394:Molecular Structure; D013194:Staining and Labeling / Q000379:methods*; D013329:Structure-Activity Relationship
chemical_list: D000970:Antineoplastic Agents; D005456:Fluorescent Dyes; D017879:Isothiocyanates; C037152:isothiocyanic acid; D001147:Arsanilic Acid