title: Up-regulation of IL-33 expression in various types of murine cells by IL-3 and IL-4.
abstract: The crucial roles of the novel cytokine IL-33 in allergic, inflammatory, infectious and autoimmune diseases are becoming characterized. However, the cytokines which regulate IL-33 expression and secretion are still largely unknown. In this study, IL-3 and IL-4 were found to up-regulate IL-33 mRNA expression in mouse peritoneal exudate cells by a two-color DNA microarray and further confirmed by real time PCR and ELISA. IL-3 and IL-4 synergistically promote IL-33 mRNA expression and IL-33 intracrine in the heterogeneous cell populations as peritoneal exudates cells, bone marrow cells and splenic cells. IL-3 and IL-4 also induced IL-33 introcrine in the peritoneal exudate cells from the macrophage-deficient op/op mice, suggesting that macrophage is not the only target of IL-3 and IL-4 in the heterogeneous peritoneal exudate cells. Furthermore, IL-3 and IL-4 were verified to promote the IL-33 intracrine in the homogeneous cell population as fibroblasts and mast cells. These results indicate that up-regulation of IL-33 expression by IL-3 and IL-4 is not a feature particular to a specific type of cells. Up to 100 cytokines were screened, but none of them stimulated the secretion or release of IL-33 in the culture system. In summary, we confirm for the first time that IL-3 and IL-4 are critical for IL-33 intracrine in murine cells of various types, indicating that IL-3 and IL-4 may play an important role in the constitutive expression of IL-33 in vivo.
authors.affiliation: Department of Microbiology and Immunology, Showa University School of Medicine, Tokyo, Japan.
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Zhao
authors.forename: Zhi-Qing
authors.initials: ZQ
authors.lastname: Hu
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007377:Interleukin-3 / Q000502:physiology*; D000067596:Interleukin-33; D015847:Interleukin-4 / Q000502:physiology*; D007378:Interleukins / Q000378:metabolism*; D015759:Ionomycin / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D020411:Oligonucleotide Array Sequence Analysis; D060888:Real-Time Polymerase Chain Reaction; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015854:Up-Regulation
chemical_list: D017931:DNA Primers; C506535:Il33 protein, mouse; D007377:Interleukin-3; D000067596:Interleukin-33; D007378:Interleukins; D008070:Lipopolysaccharides; D015847:Interleukin-4; D015759:Ionomycin; D013755:Tetradecanoylphorbol Acetate