title: Backbone resonance assignments of the C2 domain of coagulation factor VIII.
abstract: Factor VIII (FVIII, other clotting factors are named similarly) is a glycoprotein that circulates in the plasma bound to von Willebrand factor. During the blood coagulation cascade, activated FVIII (FVIIIa) binds to FIXa and activates FX in the presence of calcium ions and phospholipid membranes. The C1 and C2 domains mediate membrane binding that is essential for activation of the FVIIIa-FIXa complex. Here, (1)H, (13)C, and (15)N backbone chemical shift assignments are reported for the C2 domain of FVIII, including assignments for the residues in solvent-exposed loops. The NMR resonance assignments, along with further structural studies of membrane-bound FVIII, will advance understanding of blood-clotting protein interactions.
authors.affiliation: Department of Chemistry, University of Illinois at Urbana-Champaign, 600 S Mathews Ave, Urbana, IL 61801, USA.
authors.forename: Kristin M
authors.initials: KM
authors.lastname: Nuzzio
authors.forename: David B
authors.initials: DB
authors.lastname: Cullinan
authors.forename: Valerie A
authors.initials: VA
authors.lastname: Novakovic
authors.forename: John M
authors.initials: JM
authors.lastname: Boettcher
authors.forename: Chad M
authors.initials: CM
authors.lastname: Rienstra
authors.forename: Gary E
authors.initials: GE
authors.lastname: Gilbert
authors.forename: James D
authors.initials: JD
authors.lastname: Baleja
mesh_terms: D005169:Factor VIII / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D017434:Protein Structure, Tertiary
chemical_list: D005169:Factor VIII