title: Misexpression screen delineates novel genes controlling Drosophila lifespan.
abstract: In an initial preliminary screen we identified factors associated with controlling Drosophila aging by examining longevity in adults where EP elements induced over-expression or antisense-RNA at genes adjacent to each insertion. Here, we study 45 EP lines that initially showed at least 10% longer mean lifespan than controls. These 45 lines and a daughterless (da)-Gal4 stock were isogenized into a CS10 wild-type background. Sixteen EP lines corresponding to 15 genes significantly extended lifespan when their target genes were driven by da-Gal4. In each case, the target genes were seen to be over-expressed. Independently derived UAS-gene transgenic stocks were available or made for two candidates: ImpL2 which is ecdysone-inducible gene L2, and CG33138, 1,4-alpha-glucan branching enzyme. With both, adult lifespan was increased upon over-expression via the GeneSwitch inducible Gal4 driver system. Several genes in this set of 15 correspond to previously discovered longevity assurance systems such as insulin/IGF-1 signaling, gene silencing, and autophagy; others suggest new potential mechanisms for the control of aging including mRNA synthesis and maturation, intracellular vesicle trafficking, and neuroendocrine regulation.
authors.affiliation: Department of Physiology, College of Medicine, Korea University, 126-1 Anam-Dong 5 Ga, Seongbuk-Gu, Seoul 136-705, Republic of Korea.
authors.forename: Donggi
authors.initials: D
authors.lastname: Paik
authors.forename: Yeo Gil
authors.initials: YG
authors.lastname: Jang
authors.forename: Young Eun
authors.initials: YE
authors.lastname: Lee
authors.forename: Young Nam
authors.initials: YN
authors.lastname: Lee
authors.forename: Rochelle
authors.initials: R
authors.lastname: Yamamoto
authors.forename: Heon Yung
authors.initials: HY
authors.lastname: Gee
authors.forename: Seungmin
authors.initials: S
authors.lastname: Yoo
authors.forename: Eunkyung
authors.initials: E
authors.lastname: Bae
authors.forename: Kyung-Jin
authors.initials: KJ
authors.lastname: Min
authors.forename: Marc
authors.initials: M
authors.lastname: Tatar
authors.forename: Joong-Jean
authors.initials: JJ
authors.lastname: Park
mesh_terms: D015061:1,4-alpha-Glucan Branching Enzyme / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001343:Autophagy / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics* / Q000378:metabolism; D004742:Enhancer Elements, Genetic; D005260:Female; D005786:Gene Expression Regulation; D008136:Longevity / Q000235:genetics*; D008297:Male; D011401:Promoter Regions, Genetic; D015398:Signal Transduction / Q000235:genetics
chemical_list: D015061:1,4-alpha-Glucan Branching Enzyme