title: Diversity and physiological properties of root endophytic actinobacteria in native herbaceous plants of Korea.
abstract: Endophytic actinobacterial diversity in the native herbaceous plant species of Korea was analyzed using a culture-based approach. Sixty one actinobacterial strains were isolated, and assigned to 15 genera based on 16S rRNA gene analysis. The members of the genus Streptomyces comprised 45.9% of the total isolates, followed by Micromonospora (18.8%), Rhodococcus (6.6%), Microbispora (4.9%), and Micrococcus (4.9%). Other minor constituents included members of Microbacterium, Streptacidiphilus, Arthrobacter, Dietzia, Kitasatospora, Herbiconiux, Mycobacterium, Nocardia, Rathayibacter, and Tsukamurella. Among the isolates, 65.6% exhibited at least one hydrolytic enzyme activity out of four, and 45.9% exhibited antagonistic activity against at least one fungal pathogen out of five, thus demonstrating that endophytic actinobacteria can be an important source of bioactive compounds. Notably, most strains of Streptomyces proved active for both enzymatic and antagonistic activities.
authors.affiliation: Department of Microbiology and Molecular Biology, School of Bioscience and Biotechnology, Chungnam National University, Daejeon, 305-764, Republic of Korea.
authors.forename: Tae-Ui
authors.initials: TU
authors.lastname: Kim
authors.forename: Sung-Heun
authors.initials: SH
authors.lastname: Cho
authors.forename: Ji-Hye
authors.initials: JH
authors.lastname: Han
authors.forename: Young Min
authors.initials: YM
authors.lastname: Shin
authors.forename: Hyang Burm
authors.initials: HB
authors.lastname: Lee
authors.forename: Seung Bum
authors.initials: SB
authors.lastname: Kim
mesh_terms: D039903:Actinobacteria / Q000145:classification* / Q000302:isolation & purification / Q000502:physiology*; D000898:Antibiosis; D044822:Biodiversity; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D060026:Endophytes / Q000145:classification* / Q000302:isolation & purification / Q000502:physiology*; D004798:Enzymes / Q000378:metabolism; D005658:Fungi / Q000254:growth & development; D008969:Molecular Sequence Data; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D010946:Plants, Medicinal / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D004798:Enzymes; D012336:RNA, Ribosomal, 16S