title: IgE⁺ memory B cells and plasma cells generated through a germinal-center pathway.
abstract: Immunoglobulin E (IgE) antibodies are pathogenic in asthma and allergic diseases, but the in vivo biology of IgE-producing (IgE(+)) cells is poorly understood. A model of the differentiation of IgE(+) B cells proposes that IgE(+) cells develop through a germinal-center IgG1(+) intermediate and that IgE memory resides in the compartment of IgG1(+) memory B cells. Here we have used a reporter mouse expressing green fluorescent protein associated with membrane IgE transcripts (IgE-GFP) to assess in vivo IgE responses. In contrast to the IgG1-centered model of IgE switching and memory, we found that IgE(+) cells developed through a germinal-center IgE(+) intermediate to form IgE(+) memory B cells and plasma cells. Our studies delineate a new model for the in vivo biology of IgE switching and memory.
authors.affiliation: Department of Immunology, Genentech, South San Francisco, California, USA.
authors.forename: Oezcan
authors.initials: O
authors.lastname: Talay
authors.forename: Donghong
authors.initials: D
authors.lastname: Yan
authors.forename: Hans D
authors.initials: HD
authors.lastname: Brightbill
authors.forename: Elizabeth E M
authors.initials: EE
authors.lastname: Straney
authors.forename: Meijuan
authors.initials: M
authors.lastname: Zhou
authors.forename: Ena
authors.initials: E
authors.lastname: Ladi
authors.forename: Wyne P
authors.initials: WP
authors.lastname: Lee
authors.forename: Jackson G
authors.initials: JG
authors.lastname: Egen
authors.forename: Cary D
authors.initials: CD
authors.lastname: Austin
authors.forename: Min
authors.initials: M
authors.lastname: Xu
authors.forename: Lawren C
authors.initials: LC
authors.lastname: Wu
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology; D002454:Cell Differentiation / Q000276:immunology*; D002469:Cell Separation; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D055879:Gene Knock-In Techniques; D018858:Germinal Center / Q000166:cytology* / Q000276:immunology; D006801:Humans; D017578:Immunoglobulin Class Switching / Q000276:immunology; D007073:Immunoglobulin E / Q000276:immunology*; D007156:Immunologic Memory / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018613:Microscopy, Confocal; D010950:Plasma Cells / Q000166:cytology / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D007073:Immunoglobulin E