title: Phorbol ester or epidermal growth-factor-induced MUC5AC mucin gene expression and production from airway epithelial cells are inhibited by apigenin and wogonin.
abstract: In this study, we investigated whether apigenin and wogonin affect MUC5AC mucin production and gene expression induced by phorbol ester (phorbol 12-myristate 13-acetate, PMA) or epidermal growth factor (EGF) from human airway epithelial cells. Confluent NCI-H292 cells were pretreated with each agent for 30 min and then stimulated with PMA or EGF for 24 h, respectively. MUC5AC mucin gene expression and mucin protein production were measured by reverse transcription polymerase chain reaction (RT-PCR) and enzyme linked immunosorbent assay (ELISA). The results were as follows: (i) apigenin and wogonin were found to inhibit the production of MUC5AC mucin protein induced by PMA or EGF; (ii) both compounds also inhibited the expression of MUC5AC mucin gene induced by PMA or EGF. These results suggest that apigenin and wogonin can inhibit mucin gene expression and production of mucin protein, by directly acting on airway epithelial cells.
authors.affiliation: Pulmonology Section, Department of Internal Medicine, Chungnam National University Hospital, Daejeon, Korea.
authors.forename: Ju-Ock
authors.initials: JO
authors.lastname: Kim
authors.forename: Md Asaduzzaman
authors.initials: MA
authors.lastname: Sikder
authors.forename: Hyun Jae
authors.initials: HJ
authors.lastname: Lee
authors.forename: Mustafizur
authors.initials: M
authors.lastname: Rahman
authors.forename: Jang-Hyun
authors.initials: JH
authors.lastname: Kim
authors.forename: Gyu Tae
authors.initials: GT
authors.lastname: Chang
authors.forename: Choong Jae
authors.initials: CJ
authors.lastname: Lee
mesh_terms: D047310:Apigenin / Q000494:pharmacology*; D002460:Cell Line; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D044950:Flavanones / Q000494:pharmacology*; D006801:Humans; D055271:Mucin 5AC / Q000235:genetics / Q000378:metabolism*; D010703:Phorbol Esters / Q000494:pharmacology
chemical_list: D044950:Flavanones; C524678:MUC5AC protein, human; D055271:Mucin 5AC; D010703:Phorbol Esters; D004815:Epidermal Growth Factor; D047310:Apigenin; C085514:wogonin