title: Rapid identification of bio-molecules applied for detection of biosecurity agents using rolling circle amplification.
abstract: Detection and identification of pathogens in environmental samples for biosecurity applications are challenging due to the strict requirements on specificity, sensitivity and time. We have developed a concept for quick, specific and sensitive pathogen identification in environmental samples. Target identification is realized by padlock- and proximity probing, and reacted probes are amplified by RCA (rolling-circle amplification). The individual RCA products are labeled by fluorescence and enumerated by an instrument, developed for sensitive and rapid digital analysis. The concept is demonstrated by identification of simili biowarfare agents for bacteria (Escherichia coli and Pantoea agglomerans) and spores (Bacillus atrophaeus) released in field.
authors.affiliation: tRudbeck Laboratory, Department of Immunology, Genetics and Pathology, SciLifeLab, Uppsala University, Uppsala, Sweden.
authors.forename: Jenny
authors.initials: J
authors.lastname: Göransson
authors.forename: Rongqin
authors.initials: R
authors.lastname: Ke
authors.forename: Rachel Yuan
authors.initials: RY
authors.lastname: Nong
authors.forename: W Mathias
authors.initials: WM
authors.lastname: Howell
authors.forename: Anna
authors.initials: A
authors.lastname: Karman
authors.forename: Jan
authors.initials: J
authors.lastname: Grawé
authors.forename: Johan
authors.initials: J
authors.lastname: Stenberg
authors.forename: Malin
authors.initials: M
authors.lastname: Granberg
authors.forename: Magnus
authors.initials: M
authors.lastname: Elgh
authors.forename: David
authors.initials: D
authors.lastname: Herthnek
authors.forename: Per
authors.initials: P
authors.lastname: Wikström
authors.forename: Jonas
authors.initials: J
authors.lastname: Jarvius
authors.forename: Mats
authors.initials: M
authors.lastname: Nilsson
mesh_terms: D001407:Bacillus / Q000235:genetics / Q000378:metabolism; D023482:Bioterrorism; D004247:DNA / Q000235:genetics; D011088:DNA Ligases / Q000378:metabolism; D004270:DNA, Circular / Q000032:analysis; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005456:Fluorescent Dyes / Q000494:pharmacology; D018613:Microscopy, Confocal / Q000379:methods; D008957:Models, Genetic; D021141:Nucleic Acid Amplification Techniques; D015345:Oligonucleotide Probes; D009841:Oligonucleotides / Q000235:genetics; D020636:Pantoea / Q000235:genetics / Q000378:metabolism
chemical_list: D004270:DNA, Circular; D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D009841:Oligonucleotides; D004247:DNA; D011088:DNA Ligases