title: CTCF binds to sites in the major histocompatibility complex that are rapidly reconfigured in response to interferon-gamma.
abstract: Activation of the major histocompatibility complex (MHC) by interferon-gamma (IFN-γ) is a fundamental step in the adaptive immune response to pathogens. Here, we show that reorganization of chromatin loop domains in the MHC is evident within the first 30 min of IFN-γ treatment of fibroblasts, and that further dynamic alterations occur up to 6 h. These very rapid changes occur at genomic sites which are occupied by CTCF and are close to IFN-γ-inducible MHC genes. Early responses to IFN-γ are thus initiated independently of CIITA, the master regulator of MHC class II genes and prepare the MHC for subsequent induction of transcription.
authors.affiliation: Blizard Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, Newark St, London E1 2AT, UK.
authors.forename: Diego
authors.initials: D
authors.lastname: Ottaviani
authors.forename: Elliott
authors.initials: E
authors.lastname: Lever
authors.forename: Shihong
authors.initials: S
authors.lastname: Mao
authors.forename: Rossitza
authors.initials: R
authors.lastname: Christova
authors.forename: Babatunji W
authors.initials: BW
authors.lastname: Ogunkolade
authors.forename: Tania A
authors.initials: TA
authors.lastname: Jones
authors.forename: Jaroslaw
authors.initials: J
authors.lastname: Szary
authors.forename: Johan
authors.initials: J
authors.lastname: Aarum
authors.forename: Muhammad A
authors.initials: MA
authors.lastname: Mumin
authors.forename: Christopher A
authors.initials: CA
authors.lastname: Pieri
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Krawetz
authors.forename: Denise
authors.initials: D
authors.lastname: Sheer
mesh_terms: D001665:Binding Sites; D000076246:CCCTC-Binding Factor; D002478:Cells, Cultured; D002843:Chromatin / Q000737:chemistry / Q000187:drug effects; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D008285:Major Histocompatibility Complex; D045170:Matrix Attachment Regions / Q000187:drug effects; D012097:Repressor Proteins / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D000076246:CCCTC-Binding Factor; C000619697:CTCF protein, human; D002843:Chromatin; D012097:Repressor Proteins; D014157:Transcription Factors; D007371:Interferon-gamma