title: Chitin and L(+)-lactic acid production from crab (Callinectes bellicosus) wastes by fermentation of Lactobacillus sp. B2 using sugar cane molasses as carbon source.
abstract: Crab wastes are employed for simultaneous production of chitin and L(+)-lactic acid by submerged fermentation of Lactobacillus sp. B2 using sugar cane molasses as carbon source. Response surface methodology was applied to design the culture media considering demineralization. Fermentations in stirred tank reactor (2L) using selected conditions produced 88% demineralization and 56% deproteinization with 34% yield of chitin and 19.5 gL(-1) of lactic acid (77% yield). The chitin purified from fermentation displayed 95% degree of acetylation and 0.81 and 1 ± 0.125% of residual ash and protein contents, respectively.
authors.affiliation: Department of Biotechnology, Universidad Autonoma Metropolitana, Col. Vicentina, Mexico City, Mexico.
authors.forename: Belem
authors.initials: B
authors.lastname: Flores-Albino
authors.forename: Ladislao
authors.initials: L
authors.lastname: Arias
authors.forename: Jorge
authors.initials: J
authors.lastname: Gómez
authors.forename: Alberto
authors.initials: A
authors.lastname: Castillo
authors.forename: Miquel
authors.initials: M
authors.lastname: Gimeno
authors.forename: Keiko
authors.initials: K
authors.lastname: Shirai
mesh_terms: D000818:Animals; D019149:Bioreactors; D002244:Carbon / Q000378:metabolism*; D002686:Chitin / Q000096:biosynthesis*; D003445:Crustacea / Q000378:metabolism*; D003470:Culture Media; D005285:Fermentation; D019344:Lactic Acid / Q000096:biosynthesis*; D007778:Lactobacillus / Q000378:metabolism*; D008965:Molasses; D031786:Saccharum / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D003470:Culture Media; D002686:Chitin; D019344:Lactic Acid; D002244:Carbon