title: [Interacting insulators from the Drosophila melanogaster bithorax complex can form independent expression domains].
abstract: Regulatory region of three bithorax complex genes, Ultrabithorax (Ubx), abdominal-A (abd-A), and Abdominal-B (Abd-B) can be divided into nine iab domains, capable of directing expression of one of the genes in certain abdominal parasegment of Drosophila. In the Abd-B regulatory region, three insulators were identified, including Fab-7 and Fab-8, which flanked the iab-7domain, and Mcp, which separated the Abd-B and abd-A regulatory regions. It was suggested that boundary insulators formed a barrier between active and repressed chromatin. In the present study, using the yellow and white reporter genes and different combinations of known insulators, Mcp, Fab-7, and Fab-8, it was demonstrated that only specific interaction of two insulators was capable of isolation of active and repressed chromatin, i.e., the formation of independent expression domains.
authors.forename: O V
authors.initials: OV
authors.lastname: Kyrchanova
authors.forename: T A
authors.initials: TA
authors.lastname: Ivleva
authors.forename: P G
authors.initials: PG
authors.lastname: Georgiev
mesh_terms: D000818:Animals; D002843:Chromatin / Q000235:genetics*; D004331:Drosophila melanogaster; D005786:Gene Expression Regulation / Q000502:physiology*; D056426:Genetic Loci / Q000502:physiology*; D038101:Insulator Elements / Q000502:physiology*; D011995:Recombination, Genetic / Q000502:physiology*
chemical_list: D002843:Chromatin