title: Localized role of CRMP1 and CRMP2 in neurite outgrowth and growth cone steering.
abstract: Collapsin response mediator protein 1 (CRMP1) and CRMP2 have been known as mediators of extracellular guidance cues such as semaphorin 3A and contribute to cytoskeletal reorganization in the axonal pathfinding process. To date, how CRMP1 and CRMP2 focally regulate axonal pathfinding in the growth cone has not been elucidated. To delineate the local functions of these CRMPs, we carried out microscale-chromophore-assisted light inactivation (micro-CALI), which enables investigation of localized molecular functions with highly spatial and temporal resolutions. Inactivation of either CRMP1 or CRMP2 in the neurite shaft led to arrested neurite outgrowth. Micro-CALI of CRMP2 in the central domain of the growth cones consistently arrested neurite outgrowth, whereas micro-CALI of CRMP1 in the same region caused significant lamellipodial retraction, followed by retardation of neurite outgrowth. Focal inactivation of CRMP1 in its half region of the growth cone resulted in the growth cone turning away from the irradiated site. Conversely, focal inactivation of CRMP2 resulted in the growth cone turning toward the irradiated site. These findings suggest different functions for CRMP1 and CRMP2 in growth cone behavior and neurite outgrowth.
authors.affiliation: Department of Molecular Pharmacology and Neurobiology, Yokohama City University Graduate School of Medicine, Japan.
authors.forename: Masakazu
authors.initials: M
authors.lastname: Higurashi
authors.forename: Masumi
authors.initials: M
authors.lastname: Iketani
authors.forename: Kohtaro
authors.initials: K
authors.lastname: Takei
authors.forename: Naoya
authors.initials: N
authors.lastname: Yamashita
authors.forename: Reina
authors.initials: R
authors.lastname: Aoki
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Kawahara
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Goshima
mesh_terms: D000818:Animals; D002642:Chick Embryo; D020439:Growth Cones / Q000378:metabolism*; D015151:Immunoblotting; D007150:Immunohistochemistry; D036341:Intercellular Signaling Peptides and Proteins / Q000032:analysis / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000378:metabolism*; D016501:Neurites / Q000378:metabolism*; D055495:Neurogenesis / Q000502:physiology*; D010750:Phosphoproteins / Q000032:analysis / Q000378:metabolism*; D000098855:Collapsin Response Mediator Protein 1
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; D010750:Phosphoproteins; D000098855:Collapsin Response Mediator Protein 1; C095114:collapsin response mediator protein-2