title: A study of clinico-pathological parameters and O⁶-methylguanine DNA methyltransferase (MGMT) promoter methylation status in the prognostication of gliosarcoma.
abstract: Gliosarcoma is a rare variant of glioblastoma multiforme (GBM) with similar clinical presentation and prognosis but a distinct genetic profile. The clinicopathological features of 22 cases of gliosarcoma were analyzed with respect to age, sex, KPS score, operative diagnosis, extent of resection and histopathological subtype (predominantly sarcomatous [PS], predominantly gliomatous [PG] or mixed). Twelve cases were PS, six were PG and four were mixed. The histological subtype did not correlate with the operative diagnosis; however, it did significantly correlate with the extent of resection (P=0.014). In 14 cases with available survival data it was found that none of the clinicopathological parameters significantly correlated with survival (P>0.05). Methyl guanine DNA methyl transferase promoter methylation studies were performed using methylation-specific PCR in 16 cases which showed a methylation rate of 31.25% (5/16). The promoter methylation status did not correlate with the histological subtype and did not significantly affect survival (P>0.05). Although gliosarcomas continue to be treated in the same way as GBM, the role of chemotherapy with temozolomide is not clear. This cohort is the largest to date to uniformly receive the Stupp's protocol which is currently "standard of care" for GBM. A median overall survival of 18.5 months is substantially higher than previous studies, suggesting that temozolomide should be included in gliosarcoma therapy.
authors.affiliation: Departments of Pathology             Radiation Oncology             Neurosurgery, All India Institute of Medical Sciences, New Delhi, India.
authors.forename: Geetika
authors.initials: G
authors.lastname: Singh
authors.forename: Supriyo
authors.initials: S
authors.lastname: Mallick
authors.forename: Vikas
authors.initials: V
authors.lastname: Sharma
authors.forename: Nikhil
authors.initials: N
authors.lastname: Joshi
authors.forename: Suvendu
authors.initials: S
authors.lastname: Purkait
authors.forename: Prerana
authors.initials: P
authors.lastname: Jha
authors.forename: Mehar Chand
authors.initials: MC
authors.lastname: Sharma
authors.forename: Vaishali
authors.initials: V
authors.lastname: Suri
authors.forename: Pramod Kumar
authors.initials: PK
authors.lastname: Julka
authors.forename: Ashok Kumar
authors.initials: AK
authors.lastname: Mahapatra
authors.forename: Manmohan
authors.initials: M
authors.lastname: Singh
authors.forename: Shashank Sharad
authors.initials: SS
authors.lastname: Kale
authors.forename: Chitra
authors.initials: C
authors.lastname: Sarkar
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D002648:Child; D004247:DNA / Q000235:genetics; D015254:DNA Modification Methylases / Q000235:genetics*; D045643:DNA Repair Enzymes / Q000235:genetics*; D023381:Endpoint Determination; D005260:Female; D005500:Follow-Up Studies; D018316:Gliosarcoma / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D006801:Humans; D007150:Immunohistochemistry; D017567:Karnofsky Performance Status; D008297:Male; D008745:Methylation; D008875:Middle Aged; D019635:Neurosurgical Procedures; D011379:Prognosis; D011401:Promoter Regions, Genetic / Q000235:genetics; D013534:Survival; D016019:Survival Analysis; D025521:Tumor Suppressor Proteins / Q000235:genetics*; D055815:Young Adult
chemical_list: D025521:Tumor Suppressor Proteins; D004247:DNA; D015254:DNA Modification Methylases; C515491:MGMT protein, human; D045643:DNA Repair Enzymes