title: Malondialdehyde level and adenosine deaminase activity in adenoid tissue of patients with OME and obstructive adenoid hypertrophy.
abstract: OBJECTIVE
  The aim of our study was to determine and compare adenosine deaminase (ADA) activity and malondialdehyde (MDA) level in adenoid tissue of patients with or without otitis media with effusion (OME).
  
  
  METHODS
  The study included 30 patients undergoing adenoidectomy due to obstructive adenoid hypertrophy (OAH) or OME. Tissue MDA level was measured by the method of Okawa with modification and tissue ADA activity by the method of Giusti. We measured, superoxide dismutase (SOD) and catalase (CAT) activities as well.
  
  
  RESULTS
  In patients with OAH, mean tissue MDA level and ADA activity were 4.13 ± 0.90 nmol/mg Pr and 0.39 ± 0.04 U/mg Pr, respectively, which were significantly higher than those of OME group (1.43 ± 0.41 nmol/mg Protein and 0.22 ± 0.04 U/mg Pr, respectively) (P<0.05). SOD and CAT activities were found to be increased in patients with OAH when compared to the OME group but they did not reach statistically significant level (P=0.06 and 0.05 respectively).
  
  
  CONCLUSIONS
  The present study showed the presence of measurable ADA activity in adenoid tissue, and also significant increases in both tissue MDA level and ADA activity in OAH tissue when compared to adenoid tissue of the patient with OME. However, the significance of changes in MDA and ADA activation in the pathogenesis of OAH requires further study.
authors.affiliation: Department of Otorhinolaryngology, School of Medicine, Afyon Kocatepe University, 03200 Afyonkarahisar, Turkey. erdoganokur@yahoo.com
authors.forename: Erdogan
authors.initials: E
authors.lastname: Okur
authors.forename: Fatma Inanc
authors.initials: FI
authors.lastname: Tolun
authors.forename: Ilhami
authors.initials: I
authors.lastname: Yildirim
authors.forename: Metin
authors.initials: M
authors.lastname: Kilinc
authors.forename: M Akif
authors.initials: MA
authors.lastname: Kilic
mesh_terms: D000233:Adenoidectomy; D000234:Adenoids / Q000378:metabolism* / Q000473:pathology* / Q000601:surgery; D000243:Adenosine Deaminase / Q000378:metabolism*; D000293:Adolescent; D000402:Airway Obstruction / Q000209:etiology / Q000378:metabolism* / Q000601:surgery; D016022:Case-Control Studies; D002374:Catalase / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D006984:Hypertrophy / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism*; D010034:Otitis Media with Effusion / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D008315:Malondialdehyde; D002374:Catalase; D013482:Superoxide Dismutase; D000243:Adenosine Deaminase