title: Tissue regeneration in vivo within recombinant spidroin 1 scaffolds.
abstract: One of the major tasks of tissue engineering is to produce tissue grafts for the replacement or regeneration of damaged tissue, and natural and recombinant silk-based polymer scaffolds are promising candidates for such grafts. Here, we compared two porous scaffolds made from different silk proteins, fibroin of Bombyx mori and a recombinant analog of Nephila clavipes spidroin 1 known as rS1/9, and their biocompatibility and degradation behavior in vitro and in vivo. The vascularization and intergrowth of the connective tissue, which was penetrated with nerve fibers, at 8 weeks after subcutaneous implantation in Balb/c mice was more profound using the rS1/9 scaffolds. Implantation of both scaffolds into bone defects in Wistar rats accelerated repair compared to controls with no implanted scaffold at 4 weeks. Based on the number of macrophages and multinuclear giant cells in the subcutaneous area and the number of osteoclasts in the bone, regeneration was determined to be more effective after the rS1/9 scaffolds were implanted. Microscopic examination of the morphology of the matrices revealed differences in their internal microstructures. In contrast to fibroin-based scaffolds, the walls of the rS1/9 scaffolds were visibly thicker and contained specific micropores. We suggest that the porous inner structure of the rS1/9 scaffolds provided a better micro-environment for the regenerating tissue, which makes the matrices derived from the recombinant rS1/9 protein favorable candidates for future in vivo applications.
authors.affiliation: Department of Bioengineering, Lomonosov Moscow State University, Moscow, Russian Federation.
authors.forename: Mikhail M
authors.initials: MM
authors.lastname: Moisenovich
authors.forename: Olga
authors.initials: O
authors.lastname: Pustovalova
authors.forename: Julia
authors.initials: J
authors.lastname: Shackelford
authors.forename: Tamara V
authors.initials: TV
authors.lastname: Vasiljeva
authors.forename: Tatiana V
authors.initials: TV
authors.lastname: Druzhinina
authors.forename: Yana A
authors.initials: YA
authors.lastname: Kamenchuk
authors.forename: Vitaly V
authors.initials: VV
authors.lastname: Guzeev
authors.forename: Olga S
authors.initials: OS
authors.lastname: Sokolova
authors.forename: Vladimir G
authors.initials: VG
authors.lastname: Bogush
authors.forename: Vladimir G
authors.initials: VG
authors.lastname: Debabov
authors.forename: Mikhail P
authors.initials: MP
authors.lastname: Kirpichnikov
authors.forename: Igor I
authors.initials: II
authors.lastname: Agapov
mesh_terms: D016475:3T3 Cells; D000818:Animals; D012831:Bombyx; D001861:Bone Regeneration / Q000187:drug effects; D001842:Bone and Bones / Q000187:drug effects / Q000473:pathology; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D005349:Fibroins / Q000494:pharmacology*; D048091:Guided Tissue Regeneration / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D008855:Microscopy, Electron, Scanning; D018919:Neovascularization, Physiologic / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects; D010084:Oxidation-Reduction / Q000187:drug effects; D011108:Polymers / Q000737:chemistry; D016062:Porosity / Q000187:drug effects; D019919:Prosthesis Implantation; D051381:Rats; D011994:Recombinant Proteins / Q000494:pharmacology*; D040521:Subcutaneous Tissue / Q000098:blood supply / Q000187:drug effects / Q000294:innervation / Q000473:pathology; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D011108:Polymers; D011994:Recombinant Proteins; C086087:spidroin 1; D005349:Fibroins