title: Expression pattern, subcellular localization, and functional implications of ODAM in ameloblasts, odontoblasts, osteoblasts, and various cancer cells.
abstract: During tooth development and tumorigenesis, the odontogenic ameloblast-associated protein (ODAM) is involved in cellular differentiation and matrix protein production. However, the precise function of ODAM remains largely unknown. To suggest new functional roles of ODAM, we investigated the cellular expression and subcellular localization of ODAM in tooth and cancer cells. ODAM was expressed in ameloblasts, odontoblasts, and osteoblasts in vivo and in vitro. Furthermore, ODAM was localized in both the nucleus and cytoplasm of MMP-20 expressing ameloblasts and odontoblasts, but only in the cytoplasm of non-MMP-20 expressing osteoblasts. The extracellular secretion of ODAM was not observed in odontoblasts and osteoblasts, but was seen in ameloblasts. In addition, ODAM was discovered in the nucleus, cytoplasm, and extracellular matrix of various cancer cells. These results suggest that the expression pattern and subcellular localization of ODAM is highly variable and dependent on cell types and their differentiation states, and that functional correlations exist between ODAM and MMP-20. This study provides the first evidence for ODAM in multiple cellular compartments of differentiating odontogenic and cancer cell lines with important functional implications.
authors.affiliation: Department of Oral Histology-Developmental Biology, School of Dentistry and Dental Research Institute, BK 21, Seoul National University, Seoul 110-749, Republic of Korea.
authors.forename: Hye-Kyung
authors.initials: HK
authors.lastname: Lee
authors.affiliation: Department of Oral Histology-Developmental Biology, School of Dentistry and Dental Research Institute, BK 21, Seoul National University, Seoul 110-749, Republic of Korea.
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Park
authors.affiliation: Department of Oral Histology-Developmental Biology, School of Dentistry and Dental Research Institute, BK 21, Seoul National University, Seoul 110-749, Republic of Korea.
authors.forename: Hyun-Jung
authors.initials: HJ
authors.lastname: Oh
authors.affiliation: Department of Pediatric Dentistry, School of Dentistry and Dental Research Institute, BK 21, Seoul National University, Seoul 110-749, Republic of Korea.
authors.forename: Jung-Wook
authors.initials: JW
authors.lastname: Kim
authors.affiliation: Department of Dental Hygiene, Namseoul University, Cheon-An 331-707, Republic of Korea.
authors.forename: Hyun-Sook
authors.initials: HS
authors.lastname: Bae
authors.affiliation: Department of Oral Histology-Developmental Biology, School of Dentistry and Dental Research Institute, BK 21, Seoul National University, Seoul 110-749, Republic of Korea.
authors.forename: Joo-Cheol
authors.initials: JC
authors.lastname: Park
mesh_terms: D000565:Ameloblasts / Q000166:cytology / Q000378:metabolism*; D000682:Amyloid; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation / Q000235:genetics; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D007668:Kidney / Q000378:metabolism; D061986:MCF-7 Cells; D053519:Matrix Metalloproteinase 20 / Q000235:genetics / Q000378:metabolism; D051379:Mice; D009363:Neoplasm Proteins; D009804:Odontoblasts / Q000166:cytology / Q000378:metabolism*; D009805:Odontogenesis / Q000235:genetics; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D010053:Ovary / Q000378:metabolism; D011506:Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D000682:Amyloid; D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; D009363:Neoplasm Proteins; C480049:ODAM protein, human; C555400:ODAM protein, mouse; D011506:Proteins; C507111:MMP20 protein, human; D053519:Matrix Metalloproteinase 20