title: Role of xanthophylls in light harvesting in green plants: a spectroscopic investigation of mutant LHCII and Lhcb pigment-protein complexes.
abstract: The spectroscopic properties and energy transfer dynamics of the protein-bound chlorophylls and xanthophylls in monomeric, major LHCII complexes, and minor Lhcb complexes from genetically altered Arabidopsis thaliana plants have been investigated using both steady-state and time-resolved absorption and fluorescence spectroscopic methods. The pigment-protein complexes that were studied contain Chl a, Chl b, and variable amounts of the xanthophylls, zeaxanthin (Z), violaxanthin (V), neoxanthin (N), and lutein (L). The complexes were derived from mutants of plants denoted npq1 (NVL), npq2lut2 (Z), aba4npq1lut2 (V), aba4npq1 (VL), npq1lut2 (NV), and npq2 (LZ). The data reveal specific singlet energy transfer routes and excited state spectra and dynamics that depend on the xanthophyll present in the complex.
authors.affiliation: Department of Chemistry, University of Connecticut, Storrs, Connecticut 06269-3060, United States.
authors.forename: Marcel
authors.initials: M
authors.lastname: Fuciman
authors.forename: Miriam M
authors.initials: MM
authors.lastname: Enriquez
authors.forename: Tomáš
authors.initials: T
authors.lastname: Polívka
authors.forename: Luca
authors.initials: L
authors.lastname: Dall'Osto
authors.forename: Roberto
authors.initials: R
authors.lastname: Bassi
authors.forename: Harry A
authors.initials: HA
authors.lastname: Frank
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004735:Energy Transfer; D045342:Light-Harvesting Protein Complexes / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D014975:Lutein / Q000737:chemistry; D009154:Mutation; D013050:Spectrometry, Fluorescence; D024341:Xanthophylls / Q000737:chemistry*; D065146:Zeaxanthins
chemical_list: D029681:Arabidopsis Proteins; D045342:Light-Harvesting Protein Complexes; D024341:Xanthophylls; D065146:Zeaxanthins; C005613:violaxanthin; C011947:neoxanthin; D014975:Lutein