title: Effect of acupuncture on hippocampal Ref-1 expression in cerebral multi-infarction rats.
abstract: Redox effector factor (Ref-1) is a sensitive marker for oxidative cellular injury. The aim of this study was to investigate the effects of acupuncture on hippocampal Ref-1 expression in cerebral multi-infarction rats. The rats with reference memory impairment were randomly allocated to three groups: impaired group, acupuncture group and placebo acupuncture group. Moreover, normal group and sham-operated group were set as control groups. Morris water maze test showed that cerebral multi-infarction rats did not present significant changes in spatial working memory performance. Further investigation by immunohistochemistry revealed that acupunctural treatment significantly increased the expression of Ref-1 in the hippocampus of the impaired rats. These findings suggested that the spatial working memory was unaffected in the cerebral multi-infarction rats although spatial reference memory deficits were detected in our previous study; in addition, acupuncture could increase the Ref-1 expression, consequently exerting the anti-oxidant effects.
authors.affiliation: Acupuncture and Moxibustion Center, Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University, 23 Meishuguanhou Street, Dongcheng District, Beijing 100010, China. lcz623780@126.com
authors.forename: Cun-Zhi
authors.initials: CZ
authors.lastname: Liu
authors.forename: Zu-Guang
authors.initials: ZG
authors.lastname: Li
authors.forename: De-Jin
authors.initials: DJ
authors.lastname: Wang
authors.forename: Guang-Xia
authors.initials: GX
authors.lastname: Shi
authors.forename: Li-Ying
authors.initials: LY
authors.lastname: Liu
authors.forename: Qian-Qian
authors.initials: QQ
authors.lastname: Li
authors.forename: Chong
authors.initials: C
authors.lastname: Li
mesh_terms: D026881:Acupuncture; D000704:Analysis of Variance; D000818:Animals; D002544:Cerebral Infarction / Q000473:pathology*; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase / Q000378:metabolism*; D004195:Disease Models, Animal; D005106:Exploratory Behavior; D005786:Gene Expression Regulation / Q000502:physiology*; D006624:Hippocampus / Q000378:metabolism*; D008297:Male; D018782:Maze Learning; D008570:Memory, Short-Term / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar
chemical_list: C470319:Apex1 protein, rat; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase