title: Antiinflammatory properties of extracts and compounds isolated from Verbascum xanthophoeniceum Griseb.
abstract: Verbascum xanthophoeniceum Griseb. is an endemic plant of the Balkan region, a representative of the genus Verbascum used in traditional medicine for respiratory and inflammatory disorders. The objective of this study was to evaluate in vivo and in vitro the antiinflammatory action of crude extract, different fractions and pure compounds obtained from V. xanthophoeniceum Griseb. Bioactive metabolites were isolated by the use of low-pressure chromatographic separation. Crude methanol extract (CME) was applied in a model of paw oedema and different fractions and substances were tested in vitro for their effect on NO and cytokine production by peritoneal macrophages, and on the COX-1 and COX-2 expression. The CME exerted inhibition on cobra venom factor (CVF)-induced oedema in mice, in correlation with reduced alternative pathway (AP) complement activity. A highly suppressive effect was expressed by nigroside VI on IL-6 and NO production and by forsythoside B on TNF-α production. Leucosceptoside B lowered NO release and COX-1 expression in macrophages. Verbascum xanthophoeniceum could serve as a promising source of active compounds with antiinflammatory action, particularly in complement-mediated disorders.
authors.affiliation: Department of Immunology, Institute of Microbiology, Bulgarian Academy of Sciences, 1113, Sofia, Bulgaria.
authors.forename: Petya
authors.initials: P
authors.lastname: Dimitrova
authors.forename: Emanuela
authors.initials: E
authors.lastname: Kostadinova
authors.forename: Viktoriya
authors.initials: V
authors.lastname: Milanova
authors.forename: Kalina
authors.initials: K
authors.lastname: Alipieva
authors.forename: Milen
authors.initials: M
authors.lastname: Georgiev
authors.forename: Nina
authors.initials: N
authors.lastname: Ivanovska
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002109:Caffeic Acids / Q000302:isolation & purification / Q000494:pharmacology; D003170:Complement Pathway, Alternative; D051545:Cyclooxygenase 1 / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000378:metabolism; D004487:Edema / Q000188:drug therapy; D005960:Glucosides / Q000302:isolation & purification / Q000494:pharmacology; D015850:Interleukin-6 / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000187:drug effects*; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D009569:Nitric Oxide / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D032265:Verbascum / Q000737:chemistry*
chemical_list: D000893:Anti-Inflammatory Agents; D002109:Caffeic Acids; D005960:Glucosides; D015850:Interleukin-6; D008565:Membrane Proteins; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; C552409:forsythoside B; D009569:Nitric Oxide; C077623:Ptgs2 protein, mouse; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; C496512:Ptgs1 protein, mouse