title: Tigliane diterpene esters with IFN γ-inducing activity from the leaves of Aleurites fordii.
abstract: Bioactivity-guided fractionation on the leaves of Aleurites fordii led to the isolation of a new tigliane diterpene ester, 12-O-hexadecanoyl-7-oxo-5-ene-16-hydroxyphorbol-13-acetate (1) along with four known compounds, 12-O-hexadecanoyl-7-oxo-5-ene-phorbol-13-acetate (2), 12-O-hexadecanoyl-phorbol-13-acetate (3), 12-O-hexadecanoyl-16-hydroxyphorbol-13-acetate (4), and 12-O-hexadecanoyl-4-deoxy-4α-16-hydroxyphorbol-13-acetate (5). The structures of these compounds were determined by interpretation of NMR (1D and 2D) spectroscopic data and MS data. All the isolates were evaluated for their effects on the induction of IFN-γ in NK92 cells. Compounds 3 and 4 exhibited the most potent responses in IFN-γ induction, comparable to the positive control, phorbol 12-myristate 13-acetate (PMA).
authors.affiliation: University of Science & Technology, Daejeon 305-350, Republic of Korea.
authors.forename: Yi-Hua
authors.initials: YH
authors.lastname: Pei
authors.forename: Jae Wha
authors.initials: JW
authors.lastname: Kim
authors.forename: Ho-Bum
authors.initials: HB
authors.lastname: Kang
authors.forename: Hyeong-Kyu
authors.initials: HK
authors.lastname: Lee
authors.forename: Chan-Soo
authors.initials: CS
authors.lastname: Kim
authors.forename: Hyuk-Hwan
authors.initials: HH
authors.lastname: Song
authors.forename: Young-Won
authors.initials: YW
authors.lastname: Chin
authors.forename: Sei-Ryang
authors.initials: SR
authors.lastname: Oh
mesh_terms: D031284:Aleurites / Q000737:chemistry*; D000998:Antiviral Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D002460:Cell Line; D004224:Diterpenes / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D004952:Esters; D007371:Interferon-gamma / Q000096:biosynthesis; D007694:Killer Cells, Natural / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry*; D052616:Solid Phase Extraction; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D000998:Antiviral Agents; D004224:Diterpenes; D004952:Esters; D010936:Plant Extracts; D007371:Interferon-gamma; D013755:Tetradecanoylphorbol Acetate