title: Remote pharmacological preconditioning on median nerve territory increases Hsp32 expression and attenuates ischemia-reperfusion injury in rat heart.
abstract: OBJECTIVE
  The aim of this study was to test the hypothesis that remote pharmacological preconditioning (RPP) induced myocardial heat shock protein (Hsp) 32 expression and attenuated the ischemia-reperfusion (I/R) injury of the heart in rats.
  
  
  METHODS
  Animals were injected at the left median nerve territory with chloralose and urethane mixture. At different time intervals, myocardial Hsp32 gene expression was analyzed. Primary heart cultures were used to investigate the direct effect of drug mixture on Hsp32 expression.
  
  
  RESULTS
  The results showed that Hsp32 was time- and dose-dependently increased by in vivo drug mixture treatment, but not in primary cultures. RPP significantly decreased the duration of arrhythmia and incidence of stony heart in rats with subsequent I/R injury.
  
  
  CONCLUSIONS
  We conclude that RPP on the left median nerve territory induced Hsp32 gene expression in the heart and attenuates myocardial damage functionally after subsequent I/R injury.
authors.affiliation: Institute of Traditional Medicine, School of Medicine, National Yang-Ming University, Taiwan, ROC. chiujh@mailsrv.ym.edu.tw
authors.forename: Jen-Hwey
authors.initials: JH
authors.lastname: Chiu
authors.forename: Ya-Fang
authors.initials: YF
authors.lastname: Cheng
authors.forename: Jir-You
authors.initials: JY
authors.lastname: Wang
authors.forename: Cheng-Fong
authors.initials: CF
authors.lastname: Hsu
mesh_terms: D018686:Anesthetics, Intravenous / Q000627:therapeutic use*; D000818:Animals; D002478:Cells, Cultured; D002698:Chloralose / Q000627:therapeutic use*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D006321:Heart / Q000187:drug effects; D006419:Heme Oxygenase (Decyclizing) / Q000235:genetics*; D019157:Ischemic Preconditioning, Myocardial / Q000379:methods*; D008297:Male; D008475:Median Nerve / Q000187:drug effects*; D015428:Myocardial Reperfusion Injury / Q000188:drug therapy* / Q000235:genetics; D009206:Myocardium / Q000166:cytology / Q000378:metabolism; D051267:NF-E2-Related Factor 2 / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation; D014520:Urethane / Q000627:therapeutic use*
chemical_list: D018686:Anesthetics, Intravenous; D018840:HSP70 Heat-Shock Proteins; D051267:NF-E2-Related Factor 2; D012333:RNA, Messenger; D002698:Chloralose; D014520:Urethane; D006419:Heme Oxygenase (Decyclizing); C491523:Hmox1 protein, rat