title: Culture of limbal stem cells on human amniotic membrane.
abstract: Limbal stem cells (LSC) have an important role in the maintenance of the corneal surface epithelium, and autologous cultured limbal epithelial cell (HLECs) transplantations have contributed substantially to the treatment of the visually disabling condition known as LSC deficiency. A major challenge is the ability to identify LSC in vitro and in situ, and one of the major controversies in the field relates to reliable LSC markers. This study was carried out to evaluate the culture of a limbal biopsy on human amniotic membrane (HAM): directly on the chorionic side and on intact epithelium, and the expression of the stem cell associated markers: ABCG2, p63. HAM has been extensively used for ocular surface reconstruction and has properties which facilitate the growth of epithelial cells controlling inflammation and scarring.
authors.affiliation: Unit of Cryobiology, Complejo Hospitalario Universitario A Coruña, La Coruña, Spain. Esther.Rendal.Vazquez@sergas.es
authors.forename: Ma Esther
authors.initials: ME
authors.lastname: Rendal-Vázquez
authors.forename: Anahí
authors.initials: A
authors.lastname: San-Luis-Verdes
authors.forename: Ma Teresa
authors.initials: MT
authors.lastname: Yebra-Pimentel-Vilar
authors.forename: Isabel
authors.initials: I
authors.lastname: López-Rodríguez
authors.forename: Nieves
authors.initials: N
authors.lastname: Domenech-García
authors.forename: Candido
authors.initials: C
authors.lastname: Andión-Núñez
authors.forename: Francisco
authors.initials: F
authors.lastname: Blanco-García
mesh_terms: D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D018528:ATP-Binding Cassette Transporters / Q000096:biosynthesis; D000650:Amnion; D018929:Cell Culture Techniques; D003315:Cornea / Q000166:cytology; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D019573:Epithelium, Corneal / Q000166:cytology / Q000378:metabolism; D061252:Feeder Cells; D006801:Humans; D016850:Limbus Corneae / Q000166:cytology* / Q000254:growth & development* / Q000378:metabolism; D008565:Membrane Proteins / Q000096:biosynthesis; D009363:Neoplasm Proteins / Q000096:biosynthesis; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D046509:Tissue Culture Techniques
chemical_list: C116614:ABCG2 protein, human; D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D018528:ATP-Binding Cassette Transporters; C081876:CKAP4 protein, human; D008565:Membrane Proteins; D009363:Neoplasm Proteins