title: Antibody-functionalized fluid-permeable surfaces for rolling cell capture at high flow rates.
abstract: Adhesion-based cell capture on surfaces in microfluidic devices forms the basis of numerous biomedical diagnostics and in vitro assays. However, the performance of these platforms is partly limited by interfacial phenomena that occur at low Reynolds numbers. In contrast, cell homing to porous vasculature is highly effective in vivo during inflammation, stem cell trafficking, and cancer metastasis. Here, we show that a porous, fluid-permeable surface functionalized with cell-specific antibodies promotes efficient and selective cell capture in vitro. This architecture is advantageous due to enhanced transport as streamlines are diverted toward the surface. Moreover, specific cell-surface interactions are promoted due to reduced shear, allowing gentle cell rolling and arrest. Together, these synergistic effects enable highly effective cell capture at flow rates more than an order of magnitude larger than those provided by existing devices with solid surfaces.
authors.affiliation: BioMEMS Resource Center, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: Sukant
authors.initials: S
authors.lastname: Mittal
authors.forename: Ian Y
authors.initials: IY
authors.lastname: Wong
authors.forename: William M
authors.initials: WM
authors.lastname: Deen
authors.forename: Mehmet
authors.initials: M
authors.lastname: Toner
mesh_terms: D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002465:Cell Movement; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D046210:Microfluidic Analytical Techniques; D010539:Permeability; D016062:Porosity; D013499:Surface Properties; D013997:Time Factors
chemical_list: D007074:Immunoglobulin G