title: Effect of opioids on CXCL-8 production in healthy cats.
abstract: The aim of this study was to evaluate the effect of opioid exposure on CXC chemokine ligand (CXCL)-8 production in cats using whole blood culture. Morphine, buprenorphine, fentanyl, and saline control were administered intravenously to five cats and whole blood pathogen associated molecular pattern motif-induced CXCL-8 production capacity was evaluated. Morphine potentiated CXCL-8 production. To further characterize this effect of morphine, morphine was incubated with whole blood ex vivo and pathogen associated molecular pattern motif-induced CXCL-8 production capacity was measured. There was a time and concentration dependent effect on CXCL-8 production, suggesting the proinflammatory effect of morphine is at least partially mediated by direct stimulatory effects on leukocytes. Additional investigation is indicated to assess the implications of the immunomodulatory actions of opioids in cats.
authors.affiliation: Comparative Internal Medicine Laboratory, College of Veterinary Medicine, University of Missouri, 900 East Campus Drive, Columbia, MO 65211, USA.
authors.forename: M W
authors.initials: MW
authors.lastname: Harmon
authors.forename: S M
authors.initials: SM
authors.lastname: Axiak
authors.forename: D H
authors.initials: DH
authors.lastname: Yu
authors.forename: C H
authors.initials: CH
authors.lastname: Chang
authors.forename: B
authors.initials: B
authors.lastname: Fowler
authors.forename: A E
authors.initials: AE
authors.lastname: DeCLue
mesh_terms: D000701:Analgesics, Opioid / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D002415:Cats / Q000378:metabolism*; D018592:Cross-Over Studies; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects*; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism*
chemical_list: D000701:Analgesics, Opioid; D016209:Interleukin-8