title: Preparation of amyloid-like fibrils containing magnetic iron oxide nanoparticles: effect of protein aggregation on proton relaxivity.
abstract: A method to prepare amyloid-like fibrils functionalized with magnetic nanoparticles has been developed. The amyloid-like fibrils are prepared in a two step procedure, where insulin and magnetic nanoparticles are mixed simply by grinding in the solid state, resulting in a water soluble hybrid material. When the hybrid material is heated in aqueous acid, the insulin/nanoparticle hybrid material self assembles to form amyloid-like fibrils incorporating the magnetic nanoparticles. This results in magnetically labeled amyloid-like fibrils which has been characterized by Transmission Electron Microscopy (TEM) and electron tomography. The influence of the aggregation process on proton relaxivity is investigated. The prepared materials have potential uses in a range of bio-imaging applications.
authors.affiliation: Biomolecular and Organic Electronics, Department of Physics, Chemistry, and Biology (IFM), Linköping University, SE-581 83 Linköping, Sweden.
authors.forename: B Viktor
authors.initials: BV
authors.lastname: Andersson
authors.forename: Caroline
authors.initials: C
authors.lastname: Skoglund
authors.forename: Kajsa
authors.initials: K
authors.lastname: Uvdal
authors.forename: Niclas
authors.initials: N
authors.lastname: Solin
mesh_terms: D000682:Amyloid / Q000737:chemistry* / Q000648:ultrastructure; D000818:Animals; D002417:Cattle; D005290:Ferric Compounds / Q000737:chemistry*; D007328:Insulin / Q000737:chemistry; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D036103:Nanotechnology / Q000379:methods*; D011506:Proteins / Q000737:chemistry*; D011522:Protons
chemical_list: D000682:Amyloid; D005290:Ferric Compounds; D007328:Insulin; D058185:Magnetite Nanoparticles; D011506:Proteins; D011522:Protons; C000499:ferric oxide