title: [HIV-1 assembly is initiated by p17 matrix protein].
abstract: HIV-1 matrix protein (MA) is multifunctional structural protein located on N-terminus of Gag precursor p55 and responsible for its transport to plasma membrane, the site of virus assembly. Here, it has been shown that MA is cleaved from Gag precursor at early stage of the virus infection and participates in virus assembly. MA is transported into the nuclei wherein it associates with viral RNA (vRNA). The MA-vRNA complex is transported to plasma membrane. Mutant MA which lost its membranotropic signal does not reach plasma membrane and MA-vRNA complex remains in the nuclei and cytoskeleton. Thus, MA seems to deliver vRNA from the nuclei to plasma membrane through cytoskeleton initiating virus assembly.
authors.forename: G K
authors.initials: GK
authors.lastname: Vorkunova
authors.forename: S I
authors.initials: SI
authors.lastname: Lupandin
authors.forename: A G
authors.initials: AG
authors.lastname: Bukrinskaia
mesh_terms: D001692:Biological Transport; D015153:Blotting, Western; D002458:Cell Fractionation; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism / Q000821:virology; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism / Q000821:virology; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism / Q000821:virology; D015488:HIV Antigens / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015658:HIV Infections / Q000188:drug therapy / Q000821:virology; D017320:HIV Protease Inhibitors / Q000494:pharmacology; D015497:HIV-1 / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism / Q000821:virology*; D006801:Humans; D009154:Mutation; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011485:Protein Binding; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012367:RNA, Viral; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D019065:Virus Assembly / Q000502:physiology*; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D015488:HIV Antigens; D017320:HIV Protease Inhibitors; D011498:Protein Precursors; D012367:RNA, Viral; D014764:Viral Proteins; D054301:gag Gene Products, Human Immunodeficiency Virus; C054858:p17 protein, Human Immunodeficiency Virus Type 1