title: Indirect immunofluorescence staining of cultured neural cells.
abstract: Immunofluorescence is a technique allowing the visualization of a specific protein or antigen in cells or tissue sections by binding a specific antibody chemically conjugated with a fluorescent dye such as fluorescein isothiocyanate. There are two major types of immunofluorescence staining methods: (1) direct immunofluorescence staining in which the primary antibody is labeled with fluorescence dye and (2) indirect immunofluorescence staining in which a secondary antibody labeled with fluorochrome is used to recognize a primary antibody. This chapter describes procedures for the application of indirect immunofluorescence staining to neural cells in culture.
authors.affiliation: Department of Pharmacology and Anesthesiology, University of Padova, Padova, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Barbierato
authors.forename: Carla
authors.initials: C
authors.lastname: Argentini
authors.forename: Stephen D
authors.initials: SD
authors.lastname: Skaper
mesh_terms: D002478:Cells, Cultured / Q000166:cytology*; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods*; D007150:Immunohistochemistry / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D009474:Neurons / Q000166:cytology*