title: Pachyman treatment improves CD4+CD25+ Treg counts and serum interleukin 4 and interferon γ levels in a mouse model of Kawasaki disease.
abstract: The aim of this study was to explore the effect of pachyman, a mushroom extract, on CD4+CD25+ regulatory T cells (Tregs), serum interleukin 4 (IL-4) and interferon γ (IFN-γ) levels in a mouse model of Kawasaki disease. Lactobacillus casei cell wall extract was diluted to 1 mg/ml in PBS and administered to mice by intraperitoneal injection to establish a model of Kawasaki disease. Sixty female mice were used in this study, 40 of which were randomly assigned to a model (normal saline by gavage, n=20) or experimental group (200 mg/kg/day pachyman by gavage, n=20). The remaining 20 mice were disease and treatment-free, and were used as the control group. Compared to the control mice, mice in the model group exhibited a significantly lower percentage of CD4+CD25+ Tregs and significantly higher serum IL-4 and IFN-γ levels (P<0.05). However, CD4+CD25+ Tregs significantly increased and IL-4 and IFN-γ levels significantly decreased in experimental mice following pachyman treatment (P<0.05). Further analysis showed a negative correlation between CD4+CD25+ Tregs and IL-4/IFN-γ levels (P<0.05). In conclusion, pachyman improves immune function in a mouse model of Kawasaki disease by upregulating CD4+CD25+ Tregs, which may inhibit the cytokine secretion of Th1 and Th2 cells.
authors.affiliation: Department of Pediatrics, Heart Center, First Affiliated Hospital of Wenzhou Medical College, Wenzhou, Zhejiang 325000, PR China. chumpwz@126.com
authors.forename: Mao-Ping
authors.initials: MP
authors.lastname: Chu
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Zhang
authors.forename: Bao-Qing
authors.initials: BQ
authors.lastname: Li
authors.forename: Ai-Hua
authors.initials: AH
authors.lastname: Zhou
authors.forename: Xi-Wen
authors.initials: XW
authors.lastname: Chen
authors.forename: Yan
authors.initials: Y
authors.lastname: Qian
mesh_terms: D000818:Animals; D018791:CD4 Lymphocyte Count; D002473:Cell Wall / Q000737:chemistry; D045424:Complex Mixtures / Q000737:chemistry / Q000633:toxicity; D004195:Disease Models, Animal; D005260:Female; D005936:Glucans / Q000494:pharmacology*; D006801:Humans; D007371:Interferon-gamma / Q000097:blood*; D015847:Interleukin-4 / Q000097:blood*; D007780:Lacticaseibacillus casei / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D009080:Mucocutaneous Lymph Node Syndrome / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy*; D050378:T-Lymphocytes, Regulatory / Q000378:metabolism*; D018417:Th1 Cells / Q000378:metabolism; D018418:Th2 Cells / Q000378:metabolism
chemical_list: D045424:Complex Mixtures; D005936:Glucans; D015847:Interleukin-4; D007371:Interferon-gamma; C014731:pachyman