title: Improving metallomics information related to transgenic and non-transgenic soybean seeds using 2D-HPLC-ICP-MS and ESI-MS/MS.
abstract: This work reports the use of 2D-HPLC-ICP-MS to enlarge metallomics information when considering soybean seeds. Separations using size exclusion chromatography (SEC) allowed the identification of three metal fractions: the first corresponding to molecular weights from 38.1 to 181.1 kDa, the second from 8.2 to 17.2 kDa and the third from 0.4 to 3.8 kDa. In a second dimension, using anion exchange chromatography (AEX), three sub-fractions containing Fe, Mg and Mn, one containing Cu, and three containing Co, Cu, Mg, Mn and Zn were obtained. After these separations, 33 proteins were identified using the ESI-MS/MS technique, and divided into four functional categories: plant growth/cell division, protein destination and storage, metabolism and unclassified proteins. Among the identified proteins, proteins previously related to metals were found.
authors.affiliation: Spectrometry, Sample Preparation and Mechanization Group-GEPAM, Institute of Chemistry, University of Campinas-UNICAMP, P.O. Box 6154, 13083-970, Campinas, SP, Brazil.
authors.forename: Lidiane Raquel Verola
authors.initials: LR
authors.lastname: Mataveli
authors.forename: Mariana
authors.initials: M
authors.lastname: Fioramonte
authors.forename: Fabio César
authors.initials: FC
authors.lastname: Gozzo
authors.forename: Marco Aurélio Zezzi
authors.initials: MA
authors.lastname: Arruda
mesh_terms: D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D008667:Metalloproteins / Q000378:metabolism*; D008670:Metals / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D012639:Seeds / Q000235:genetics / Q000378:metabolism*; D013025:Glycine max / Q000235:genetics / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D008667:Metalloproteins; D008670:Metals; D010940:Plant Proteins