title: First isolation of Dengue 4 in the state of São Paulo, Brazil, 2011.
abstract: We report the first isolation of Dengue virus 4 (DENV-4) in the state of São Paulo, from two patients - one living in São José do Rio Preto and the other one in Paulo de Faria, both cities located in the Northwest region of the state. The virus isolations were accomplished in the clone C6/36 Aedes albopictus cell line, followed by indirect immunofluorescence assays, performed with type-specific monoclonal antibodies that showed positive reactions for DENV-4. The results were confirmed by Nested RT-PCR and Real-Time RT-PCR assays. The introduction of DENV-4 in a country that already has to deal with the transmission of three other serotypes increases the possibility of the occurrence of more severe cases of the disease. The importance of early detection of dengue cases, before the virus spreads and major outbreaks occur, should be emphasized.
authors.affiliation: Núcleo de Doenças de Transmissão Vetorial, Instituto Adolfo Lutz, São Paulo, SP, Brazil. imrocco@uol.com.br
authors.forename: Iray Maria
authors.initials: IM
authors.lastname: Rocco
authors.forename: Vivian Regina
authors.initials: VR
authors.lastname: Silveira
authors.forename: Adriana Yurika
authors.initials: AY
authors.lastname: Maeda
authors.forename: Sarai Joaquim dos Santos
authors.initials: SJ
authors.lastname: Silva
authors.forename: Carine
authors.initials: C
authors.lastname: Spenassatto
authors.forename: Ivani
authors.initials: I
authors.lastname: Bisordi
authors.forename: Akemi
authors.initials: A
authors.lastname: Suzuki
mesh_terms: D000330:Aedes / Q000821:virology; D000818:Animals; D001938:Brazil; D003715:Dengue / Q000821:virology*; D003716:Dengue Virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction