title: [Study on the composition and pharmacological function change of rhizoma polygonati before and after processing].
abstract: OBJECTIVE
  To discuss the processing mechanism of Rhizoma Polygonati (RP) through studying the correlation between the change of composition and pharmacological function in raw and processed RP.
  
  
  METHODS
  The extraction of petroleum ether, methylene dichloride, ethyl acetate and 1-butanol of the raw and processed RP were compared by HPLC. The compounds changed in processed RP in the methylene dichloride extraction were further identified with reference substances. The immune function of methylene dichloride extraction of raw and processed RP were compared.
  
  
  RESULTS
  The changed compound in concentration was determined to be 5-Hydroxymethylfurfural. After processed, the concentration of 5-Hydroxymethylfurfural sharply increased. The carbon clearance index (P < 0.01) and coefficient of phagocytosis (P < 0.05) were increased remarkably by processed RP comparing to those of the normal saline and raw RP.
  
  
  CONCLUSIONS
  The increase of immune function of processed RP may be related to increasing of concentration of 5-Hydroxymethylfurfural. The results provide a better understanding of RP processing.
authors.affiliation: Pharmacy College of Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China. ly1638163@163.com
authors.forename: Ling-Yun
authors.initials: LY
authors.lastname: Zhong
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Hui-Jun
authors.initials: HJ
authors.lastname: Huo
authors.forename: Qian-Feng
authors.initials: QF
authors.lastname: Gong
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D005662:Furaldehyde / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects; D008752:Methylene Chloride; D051379:Mice; D010587:Phagocytosis / Q000187:drug effects*; D010946:Plants, Medicinal / Q000737:chemistry; D031384:Polygonatum / Q000737:chemistry*; D027343:Rhizome / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013154:Spleen / Q000187:drug effects; D013678:Technology, Pharmaceutical / Q000379:methods
chemical_list: D004365:Drugs, Chinese Herbal; D012997:Solvents; D008752:Methylene Chloride; C008046:5-hydroxymethylfurfural; D005662:Furaldehyde