title: Inhibitory effects of epigallocatechin-3-gallate on cell proliferation             and the expression of HIF-1α and P-gp in the human pancreatic carcinoma cell line             PANC-1.
abstract: The aim of this study was to verify the inhibitory effects of epigallocatechin-3-gallate             (EGCG) on cell proliferation and the expression of hypoxia-inducible factor 1             (HIF-1α) and multidrug resistance protein 1 (MDR1/P-gp) in the human pancreatic             carcinoma cell line PANC-1, thereby, reversing drug resistance of pancreatic carcinoma             and improving its sensitivity to cancer chemotherapy. The human pancreatic carcinoma             cell line PANC-1 was incubated under hypoxic conditions with different concentrations             of epigallocatechin-3-gallate (EGCG) for indicated hours. The effects of EGCG             on the mRNA or protein expression of HIF-1α and MDR1 were determined by RT-PCR             or western blotting. Cellular proliferation and viability assays were measured             using Cell Counting Kit-8. Western blotting revealed that EGCG inhibits the expression             of the HIF-1α protein in a dose-dependent manner, while RT-PCR showed that it             does not have any effects on HIF-1α mRNA. In addition, EGCG attenuated the mRNA             and protein levels of P-gp in a dose-dependent manner, reaching a peak at the             highest concentration. Furthermore, EGCG inhibited the proliferation of PANC-1             cells in a concentration- and time-dependent manner. The attenuation of HIF-1α             and the consequently reduced P-gp could contribute to the inhibitory effects of             EGCG on the proliferation of PANC-1 cells.
authors.affiliation: Department of Gastroenterology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, PR China.
authors.forename: Zhenni
authors.initials: Z
authors.lastname: Zhu
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Zhiqing
authors.initials: Z
authors.lastname: Liu
authors.forename: Fan
authors.initials: F
authors.lastname: Wang
authors.forename: Qiu
authors.initials: Q
authors.lastname: Zhao
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics / Q000378:metabolism*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D002277:Carcinoma / Q000235:genetics / Q000378:metabolism*; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics / Q000378:metabolism*; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000970:Antineoplastic Agents; D000975:Antioxidants; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D012333:RNA, Messenger; D002392:Catechin; C045651:epigallocatechin gallate