title: Effect of N-acetylcysteine on lung injury induced by skeletal muscle ischemia-reperfusion. Histopathlogical study in rat model.
abstract: OBJECTIVE
  To investigate whether N-acetylcysteine, a free radicals scavenger has a protective effect against lung injury as a remote organ after skeletal muscle ischemia-reperfusion.
  
  
  METHODS
  Twenty Wistar male rats were divided randomly into two experimental groups: group ischemia-reperfusion (group I) and group ischemia-reperfusion +N-acetylcysteine (group II). All animals were undergone two hours of ischemia by occlusion femoral artery and 24h of reperfusion. Before clamped the femoral artery, 250 IU heparin was administered via the jugular vein to prevent clotting. Rats that were treated with N-acetylcysteine given IV at a dose of 150 mgkg(-1), immediately before reperfusion. After 24h of reperfusion, animals were euthanized and left lung harvested for histopathological analysis under light microscopy.
  
  
  RESULTS
  In the group I, tissues showed histological changes with intra-alveolar edema, intra-alveolar hemorrhage and neutrophilic infiltration. Histopathologically, there was a significant difference (P = 0.005) between two groups.
  
  
  CONCLUSIONS
  Administration of N-acetylcysteine treatment significantly decreased lung injury induced by skeletal muscle ischemia reperfusion according to histological findings.
authors.affiliation: Faculty of Veterinary Science, Kahnooj Branch, Islamic Azad University, Kerman, Iran.
authors.forename: Amir
authors.initials: A
authors.lastname: Sotoudeh
authors.forename: Mohammad Ashrafzadeh
authors.initials: MA
authors.lastname: Takhtfooladi
authors.forename: Amirali
authors.initials: A
authors.lastname: Jahanshahi
authors.forename: Adel Haghighi Khiabanian
authors.initials: AH
authors.lastname: Asl
authors.forename: Hamed Ashrafzadeh
authors.initials: HA
authors.lastname: Takhtfooladi
authors.forename: Mohammadreza
authors.initials: M
authors.lastname: Khansari
mesh_terms: D000111:Acetylcysteine / Q000627:therapeutic use*; D000818:Animals; D004195:Disease Models, Animal; D016166:Free Radical Scavengers / Q000627:therapeutic use*; D008168:Lung / Q000187:drug effects / Q000473:pathology; D055370:Lung Injury / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D018482:Muscle, Skeletal; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000473:pathology / Q000517:prevention & control*; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D016166:Free Radical Scavengers; D000111:Acetylcysteine