title: MTHFR C677T, MTHFR A1298C, and OPG A163G polymorphisms in Mexican patients with rheumatoid arthritis and osteoporosis.
abstract: MTHFR polymorphisms C677T and A1298C are associated with reduced MTHFR enzyme activity and hyperhomocysteinemia, which has been associated with osteoporosis. The A163G polymorphism in osteoprotegerin (OPG) has been studied in osteoporosis with controversial results. The objective of the present study was to investigate the association(s) among MTHFR C677T, MTHFR A1298C, and OPG A163G polymorphisms in Mexican patients with rheumatoid arthritis and osteoporosis. The femoral neck and lumbar spine bone mineral densities (BMDs) were measured in 71 RA patients, and genotyping for the three polymorphisms was performed via restriction fragment length polymorphism analysis. Patients with osteoporosis/osteopenia exhibited statistically significant differences in the genotype frequencies of MTHFR C677T as well as an association with femoral neck BMD; TT homozygotes had lower BMDs than patients with the CT genotype, and both of these groups had lower BMDs than patients with the CC genotype. The associations of the MTHFR C677T polymorphism with osteoporosis/osteopenia and femoral neck BMD suggest that these polymorphisms confer a risk of developing osteoporosis in patients with rheumatoid arthritis, a risk that may be reduced with folate and B complex supplementation.
authors.affiliation: Doctorado en Genética Humana, Instituto de Genética Humana, CUCS, Universidad de Guadalajara and División de Genética, CIBO, Instituto Mexicano del Seguro Social (IMSS), Jalisco, México.
authors.forename: Aniel Jessica Leticia
authors.initials: AJ
authors.lastname: Brambila-Tapia
authors.forename: Jorge
authors.initials: J
authors.lastname: Durán-González
authors.forename: Lucila
authors.initials: L
authors.lastname: Sandoval-Ramírez
authors.forename: Juan Pablo
authors.initials: JP
authors.lastname: Mena
authors.forename: Mario
authors.initials: M
authors.lastname: Salazar-Páramo
authors.forename: Jorge Iván
authors.initials: JI
authors.lastname: Gámez-Nava
authors.forename: Laura
authors.initials: L
authors.lastname: González-López
authors.forename: Brissia
authors.initials: B
authors.lastname: Lazalde-Medina B
authors.forename: Nory Omayra
authors.initials: NO
authors.lastname: Dávalos
authors.forename: Valeria
authors.initials: V
authors.lastname: Peralta-Leal
authors.forename: Mónica
authors.initials: M
authors.lastname: Vázquez del Mercado
authors.forename: Claudia Patricia
authors.initials: CP
authors.lastname: Beltrán-Miranda
authors.forename: Ingrid Patricia
authors.initials: IP
authors.lastname: Dávalos
mesh_terms: D000328:Adult; D000368:Aged; D001172:Arthritis, Rheumatoid / Q000201:enzymology / Q000235:genetics*; D015519:Bone Density; D005260:Female; D005272:Femur Neck / Q000473:pathology; D056726:Genetic Association Studies; D006239:Haplotypes; D006801:Humans; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D008800:Mexico; D008875:Middle Aged; D010024:Osteoporosis / Q000201:enzymology / Q000235:genetics*; D053244:Osteoprotegerin / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D018709:Statistics, Nonparametric
chemical_list: D053244:Osteoprotegerin; C506030:TNFRSF11B protein, human; D042965:Methylenetetrahydrofolate Reductase (NADPH2)