title: The SUMO protease SENP7 is a critical component to ensure HP1 enrichment at pericentric heterochromatin.
abstract: SUMOylation promotes targeting of HP1α to pericentric heterochromatin. Here we identify the SUMO-specific protease SENP7 in mouse as a maintenance factor for HP1α accumulation at this location. SENP7 interacts directly with HP1α, localizes at HP1-enriched pericentric domains and can deconjugate SUMOylated HP1α in vivo. Depletion of SENP7 delocalizes HP1α from pericentric heterochromatin without affecting H3K9me3 levels. We propose that following targeting of HP1α, a subsequent deSUMOylation event enables HP1α retention at these domains.
authors.affiliation: Institut Curie, Centre de Recherche, Paris, France.
authors.forename: Christèle
authors.initials: C
authors.lastname: Maison
authors.forename: Kelly
authors.initials: K
authors.lastname: Romeo
authors.forename: Delphine
authors.initials: D
authors.lastname: Bailly
authors.forename: Marion
authors.initials: M
authors.lastname: Dubarry
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Quivy
authors.forename: Geneviève
authors.initials: G
authors.lastname: Almouzni
mesh_terms: D000818:Animals; D000090266:Chromobox Protein Homolog 5; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis* / Q000378:metabolism*; D010450:Endopeptidases / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D006570:Heterochromatin / Q000378:metabolism*; D051379:Mice; D041681:NIH 3T3 Cells; D025842:SUMO-1 Protein / Q000378:metabolism*
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D006570:Heterochromatin; D025842:SUMO-1 Protein; D000090266:Chromobox Protein Homolog 5; D010450:Endopeptidases; C572283:SENP7 protein, mouse