title: Pulsed laser triggered high speed microfluidic fluorescence activated cell sorter.
abstract: We report a high speed and high purity pulsed laser triggered fluorescence activated cell sorter (PLACS) with a sorting throughput up to 20,000 mammalian cells s(-1) with 37% sorting purity, 90% cell viability in enrichment mode, and >90% purity in high purity mode at 1500 cells s(-1) or 3000 beads s(-1). Fast switching (30 μs) and a small perturbation volume (~90 pL) is achieved by a unique sorting mechanism in which explosive vapor bubbles are generated using focused laser pulses in a single layer microfluidic PDMS channel.
authors.affiliation: Department of Mechanical and Aerospace Engineering, University of California, Los Angeles (UCLA), 420 Westwood Plaza, Rm 37-138, Los Angeles, CA 90095, USA.
authors.forename: Ting-Hsiang
authors.initials: TH
authors.lastname: Wu
authors.forename: Yue
authors.initials: Y
authors.lastname: Chen
authors.forename: Sung-Yong
authors.initials: SY
authors.lastname: Park
authors.forename: Jason
authors.initials: J
authors.lastname: Hong
authors.forename: Tara
authors.initials: T
authors.lastname: Teslaa
authors.forename: Jiang F
authors.initials: JF
authors.lastname: Zhong
authors.forename: Dino
authors.initials: D
authors.lastname: Di Carlo
authors.forename: Michael A
authors.initials: MA
authors.lastname: Teitell
authors.forename: Pei-Yu
authors.initials: PY
authors.lastname: Chiou
mesh_terms: D045744:Cell Line, Tumor; D002469:Cell Separation; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D007834:Lasers; D046210:Microfluidic Analytical Techniques
chemical_list: D004129:Dimethylpolysiloxanes; D005456:Fluorescent Dyes; C013830:baysilon