title: Effects of cigarette smoke and ethanol intake on mouse oesophageal mucosa changes induced by dietary zinc deficiency and deoxycholic acid supplementation.
abstract: The noxious effects of dietary zinc deficiency (ZD) and deoxycholic bile acid (DCA) supplementation in the oesophagus were investigated. The additional influence of cigarette smoke and ethanol intake on the changes in the oesophageal mucosa induced by dietary ZD plus DCA was also assessed. Male C57BL/6 mice were allocated into four groups: Group 1 was fed control diet and groups 2-4 were fed ZD plus DCA diet. After 5 weeks, groups 3 and 4 were exposed to 10% ethanol intake or cigarette smoke for 15 weeks, respectively. All animals were euthanized at the end of week 20, and the oesophagus, lung, liver and colon were collected and analysed by conventional morphology. Cell proliferation was assessed in the oesophageal mucosa by Ki-67 immunohistochemistry and cyclooxygenase 2 (COX-2) protein by Western blotting. Dietary ZD plus DCA treatment induced mild hyperkeratosis and hyperplasia, increased cell proliferation index and COX-2 protein expression in the oesophagus, and intranuclear inclusion, karyocytomegaly and microvesicular fatty change in the liver. Cigarette smoke increased COX-2 protein expression in oesophageal mucosa and irregular enlargement of alveolus and alveolar ductal air spaces, while ethanol enhanced liver damage induced by ZD plus DCA diet. These findings indicate that dietary ZD plus DCA treatment during 20 weeks induces a pattern of chemical oesophageal injury but not Barrett's-like lesions.
authors.affiliation: Department of Morphology, Institute of Biosciences, UNESP, São Paulo State University, Botucatu, SP, Brazil.
authors.forename: Joyce R
authors.initials: JR
authors.lastname: Zapaterini
authors.forename: Nelci A
authors.initials: NA
authors.lastname: de Moura
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Ribeiro
authors.forename: Maria A M
authors.initials: MA
authors.lastname: Rodrigues
authors.forename: Luis F
authors.initials: LF
authors.lastname: Barbisan
mesh_terms: D000818:Animals; D015153:Blotting, Western; D049109:Cell Proliferation / Q000187:drug effects; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D003840:Deoxycholic Acid / Q000008:administration & dosage* / Q000009:adverse effects; D004032:Diet; D019587:Dietary Supplements / Q000009:adverse effects*; D004947:Esophagus / Q000187:drug effects / Q000473:pathology*; D000431:Ethanol / Q000009:adverse effects*; D007150:Immunohistochemistry; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008108:Liver Diseases, Alcoholic / Q000209:etiology / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009092:Mucous Membrane / Q000187:drug effects / Q000473:pathology; D012906:Smoke / Q000009:adverse effects*; D014026:Nicotiana / Q000009:adverse effects; D015032:Zinc / Q000008:administration & dosage / Q000009:adverse effects / Q000172:deficiency*
chemical_list: D012906:Smoke; D003840:Deoxycholic Acid; D000431:Ethanol; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2; D015032:Zinc