title: Preparation of gentamicin-loaded electrospun coating on titanium implants and a study of their properties in vitro.
abstract: OBJECTIVE
  Implant-related infections are disastrous complications in the clinic, and there are no effective therapies. In this preliminary study, gentamicin-loaded coating on titanium implants was prepared using the electrospinning technique, and some properties of the coating titanium implants were studied.
  
  
  METHODS
  We adopted the electrospinning technique to prepare gentamicin-coated titanium implants. The surface structure of the coating implants was observed using scanning electron microscope. An elution study was performed to determine the release behavior of the gentamicin from the coating. The antibacterial efficacy and quantitative analysis of the bacterial adhesion of Staphylococcus aureus were evaluated in vitro, and the cytotoxicity of the coated titanium implants on osteoblasts was investigated in vitro.
  
  
  RESULTS
  The morphology of the gentamicin-coated titanium implants exhibited nanofibers, and the release of gentamicin showed an initial gentamicin burst followed by a slow release. The gentamicin-coated titanium implants had a persistent antibacterial efficacy for 1 week and significantly reduced the adhesion of the Staphylococcus aureus compared with bare titanium implants in vitro. There was no cytotoxicity observed in vitro for the gentamicin-coated implants.
  
  
  CONCLUSIONS
  The gentamicin-coated titanium implants, which were prepared using an electrospinning technique, present many advantages and may be considered to prevent and treat the implant-related infections.
authors.affiliation: Department of Orthopaedics, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China.
authors.forename: Liang-liang
authors.initials: LL
authors.lastname: Li
authors.forename: Li-ming
authors.initials: LM
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.forename: Lan-xin
authors.initials: LX
authors.lastname: Lv
mesh_terms: D000704:Analysis of Variance; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D001422:Bacterial Adhesion / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D005839:Gentamicins / Q000737:chemistry*; D066298:In Vitro Techniques; D008855:Microscopy, Electron, Scanning; D019736:Prostheses and Implants; D016459:Prosthesis-Related Infections / Q000517:prevention & control*; D013211:Staphylococcus aureus / Q000187:drug effects*; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D005839:Gentamicins; D014025:Titanium