title: Laccase production by the aquatic ascomycete Phoma sp. UHH 5-1-03 and the white rot basidiomycete Pleurotus ostreatus DSM 1833 during submerged cultivation on banana peels and enzyme applicability for the removal of endocrine-disrupting chemicals.
abstract: This work aimed to study the production of laccase from Pleurotus ostreatus DSM 1833 and Phoma sp. UHH 5-1-03 using banana peels as alternative carbon source, the subsequent partial purification and characterization of the enzyme, as well the applicability to degrade endocrine disruptors. The laccase stability with pH and temperature, the optimum pH, the K (m) and V(max) parameters, and the molar mass were determined. Tests were conducted for assessing the ability of degradation of the endocrine disruptors t-nonylphenol, bisphenol A, and 17α-ethinylestradiol. Laccase production of 752 and 1,117 U L⁻¹ was obtained for Phoma sp. and P. ostreatus, respectively. Phoma sp. laccase showed higher stability with temperature and pH. The laccase from both species showed higher affinity by syringaldazine. The culture broth with banana peels induced the production of two isoforms of P. ostreatus (58.7 and 21 kDa) and one of Phoma sp. laccase (72 kDa). In the first day of incubation, the concentrations of bisphenol A and 17α-ethinylestradiol were reduced to values close to zero and after 3 days the concentration of t-nonylphenol was reduced in 90% by the P. ostreatus laccase, but there was no reduction in its concentration by the Phoma sp. laccase.
authors.affiliation: UFZ, Department of Environmental Microbiology, UFZ, Helmholz Centre for Environmental Research, Permoserstrasse 15, 04318 Leipzig, Germany.
authors.forename: Nelson
authors.initials: N
authors.lastname: Libardi
authors.forename: Regina Maria Miranda
authors.initials: RM
authors.lastname: Gern
authors.forename: Sandra Aparecida
authors.initials: SA
authors.lastname: Furlan
authors.forename: Dietmar
authors.initials: D
authors.lastname: Schlosser
mesh_terms: D001203:Ascomycota / Q000254:growth & development / Q000378:metabolism*; D055162:Biocatalysis; D001673:Biodegradation, Environmental; D046508:Culture Techniques / Q000379:methods*; D052244:Endocrine Disruptors / Q000302:isolation & purification* / Q000378:metabolism; D004795:Enzyme Stability; D005285:Fermentation; D007101:Immersion; D007220:Industrial Waste; D042845:Laccase / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D028521:Musa / Q000378:metabolism*; D019441:Plant Epidermis / Q000378:metabolism*; D020076:Pleurotus / Q000254:growth & development / Q000378:metabolism*
chemical_list: D052244:Endocrine Disruptors; D007220:Industrial Waste; D042845:Laccase