title: Importance of Micromonospora spp. as colonizers of cellulose in freshwater lakes as demonstrated by quantitative reverse transcriptase PCR of 16S rRNA.
abstract: The relative abundance of micromonosporas in the bacterial communities inhabiting cellulose baits, water columns, and sediments of two freshwater lakes was determined by quantitative PCR (qPCR) of reverse-transcribed 16S rRNA. Micromonospora spp. were shown to be significant members of the active bacterial population colonizing cellulosic substrates in the lake sediment, and their increased prevalence with greater depth was confirmed by enumeration of CFU.
authors.affiliation: Microbiology Research Group, Institute of Integrative Biology, University of Liverpool, Liverpool, United Kingdom.
authors.forename: Alexandre B
authors.initials: AB
authors.lastname: de Menezes
authors.forename: James E
authors.initials: JE
authors.lastname: McDonald
authors.forename: Heather E
authors.initials: HE
authors.lastname: Allison
authors.forename: Alan J
authors.initials: AJ
authors.lastname: McCarthy
mesh_terms: D058491:Bacterial Load / Q000379:methods; D002482:Cellulose / Q000378:metabolism*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005618:Fresh Water / Q000382:microbiology*; D019015:Geologic Sediments / Q000382:microbiology*; D008847:Micromonospora / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D002482:Cellulose