title: A novel glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter for expressing transgenes in the halotolerant alga Dunaliella salina.
abstract: A major challenge for efficient transgene expression in Dunaliella salina is to find strong endogenous promoters to drive the transgene expression. In the present study, a novel glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter was cloned and used to drive expressions of the bialaphos resistance (bar) gene and of the N-terminal fragment of human canstatin (Can-N). The results showed that the bar gene was transcribed by the GAPDH promoter and integrated into the genome of the transformants of D. salina. Furthermore, the PCR identification, Southern and western blots indicated that Can-N was expressed in transgenic D. salina, demonstrating that the promoter of the D. salina                         GAPDH gene is suitable for driving expression of heterologous genes in transgenic D. salina.
authors.affiliation: Institute of Tumor Molecular Surgery, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, 450052, Henan, China.
authors.forename: Yanlong
authors.initials: Y
authors.lastname: Jia
authors.forename: Shenke
authors.initials: S
authors.lastname: Li
authors.forename: George
authors.initials: G
authors.lastname: Allen
authors.forename: Shuying
authors.initials: S
authors.lastname: Feng
authors.forename: Lexun
authors.initials: L
authors.lastname: Xue
mesh_terms: D001483:Base Sequence; D000460:Chlorophyta / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D024141:Collagen Type IV / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D009943:Organophosphorus Compounds / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D012965:Sodium Chloride / Q000378:metabolism; D019076:Transgenes
chemical_list: C502625:COL4A2 protein, human; D024141:Collagen Type IV; D009943:Organophosphorus Compounds; C003122:bialaphos; D012965:Sodium Chloride; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases