title: [Evaluation of molecular biology reagents used in plcR-tergeted RSI-PCR assay for B. anthracis identification and their influence on time necessary for obtaining results].
abstract: Fast and reliable identification of B. anthracis is crucial for a successful therapy of persons exposed to anthrax spores. Use of molecular biology techniques significantly reduces time necessary for obtaining results. However, the molecular identification is hampered by the high genetic similarity of the B. cereus group bacteria. A lot of published B. antharcis identification approaches turned out to be non-specific. Nevertheless, theplcR-targeted RSI-PCR assay described in 2007 is still regarded as highly specific for anthrax identification. In this paper possibility of significant reduction of time necessary for obtaining results by the use of modern, "fast" polymerases and restriction enzymes will be presented. The use of a such reagents enable to reduce time of the plcR-targeted RSI-PCR assay to about two hours.
authors.affiliation: Zakład Bakteriologii Narodowego Instytutu Zdrowia Publicznego-Państwowego Zakładu Higieny w Warszawie.
authors.forename: Aleksandra A
authors.initials: AA
authors.lastname: Zasada
mesh_terms: D001408:Bacillus anthracis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D001409:Bacillus cereus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis; D003937:Diagnosis, Differential; D007202:Indicators and Reagents; D016133:Polymerase Chain Reaction / Q000295:instrumentation* / Q000379:methods; D013997:Time Factors
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D007202:Indicators and Reagents