title: UHPLC-MS analysis of juvenile hormone II in Mediterranean corn borer (Sesamia nonagrioides) hemolymph using various ionization techniques.
abstract: The juvenile hormones (JHs) have been considered the most versatile hormones in the animal kingdom. JH-II is the most abundant JH in Sesamia nonagrioides, important maize pests in the Mediterranean basin. This study compared the sensitivities and matrix effects of four ionization modes on analyzing JH-II in S. nonagrioides hemolymph using ultrahigh-performance liquid chromatography-mass spectrometry (UHPLC-MS) in single ion monitoring (SIM) mode. The ionization techniques tested were electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI), atmospheric pressure photoionization (APPI), and APPI with the lamp turned off, which corresponds to atmospheric pressure thermospray ionization (APTSI). ESI was discarded because of the high matrix effect. APPI was discarded because the correlation responses between solvent and matrix on the instrumental quality parameters were worse than those for APTSI and APCI. In our analytical conditions, APCI has shown the best validation parameter values. APCI ionization is widely available in instrumental laboratories.
authors.affiliation: Scientific Technical Services, University of Lleida, PCiTAL, 25003-Lleida, Spain.
authors.forename: Francisca
authors.initials: F
authors.lastname: Vilaró
authors.forename: Meritxell
authors.initials: M
authors.lastname: Pérez-Hedo
authors.forename: Jordi
authors.initials: J
authors.lastname: Eras
authors.forename: Ramon
authors.initials: R
authors.lastname: Canela
authors.forename: Matilde
authors.initials: M
authors.lastname: Eizaguirre
mesh_terms: D000818:Animals; D001274:Atmospheric Pressure; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006458:Hemolymph / Q000737:chemistry*; D007477:Ions / Q000737:chemistry; D007605:Juvenile Hormones / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D009036:Moths / Q000737:chemistry*; D015203:Reproducibility of Results; D012717:Sesquiterpenes / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D003313:Zea mays
chemical_list: D007477:Ions; D007605:Juvenile Hormones; D012717:Sesquiterpenes; C055442:juvenile hormone II