title: Functionally relevant neutrophilia in CD11c diphtheria toxin receptor transgenic mice.
abstract: Transgenic mice expressing the diphtheria toxin receptor (DTR) in specific cell types are key tools for functional studies in several biological systems. B6.FVB-Tg(Itgax-DTR/EGFP)57Lan/J (CD11c.DTR) and B6.Cg-Tg(Itgax-DTR/OVA/EGFP)1Gjh/Crl (CD11c.DOG) mice express the DTR in CD11c(+) cells, allowing conditional depletion of dendritic cells. We report that dendritic-cell depletion in these models caused polymorphonuclear neutrophil (PMN) release from the bone marrow, which caused chemokine-dependent neutrophilia after 6-24 h and increased bacterial clearance in a mouse pyelonephritis model. We present a transgenic mouse line, B6.Cg-Tg(Itgax-EGFP-CRE-DTR-LUC)2Gjh/Crl (CD11c.LuciDTR), which is unaffected by early neutrophilia. However, CD11c.LuciDTR and CD11c.DTR mice showed late neutrophilia 72 h after dendritic cell depletion, which was independent of PMN release and possibly resulted from increased granulopoiesis. Thus, the time point of dendritic cell depletion and the choice of DTR transgenic mouse line must be considered in experimental settings where neutrophils may be involved.
authors.affiliation: Institutes of Molecular Medicine and Experimental Immunology, Rheinische Friedrich Wilhelms University, Bonn, Germany.
authors.forename: André P
authors.initials: AP
authors.lastname: Tittel
authors.forename: Christoph
authors.initials: C
authors.lastname: Heuser
authors.forename: Christina
authors.initials: C
authors.lastname: Ohliger
authors.forename: Chrystel
authors.initials: C
authors.lastname: Llanto
authors.forename: Simon
authors.initials: S
authors.lastname: Yona
authors.forename: Günter J
authors.initials: GJ
authors.lastname: Hämmerling
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Engel
authors.forename: Natalio
authors.initials: N
authors.lastname: Garbi
authors.forename: Christian
authors.initials: C
authors.lastname: Kurts
mesh_terms: D000818:Animals; D039521:CD11c Antigen / Q000235:genetics / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D004167:Diphtheria Toxin / Q000494:pharmacology; D004195:Disease Models, Animal; D005260:Female; D008264:Macrophages; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D018448:Models, Immunological; D009504:Neutrophils / Q000166:cytology / Q000276:immunology*; D011704:Pyelonephritis / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D056689:Uropathogenic Escherichia coli / Q000276:immunology / Q000502:physiology
chemical_list: D039521:CD11c Antigen; D004167:Diphtheria Toxin