title: Determination of enrofloxacin by room-temperature phosphorimetry after solid phase extraction on an acrylic polymer sorbent.
abstract: A phosphorimetric method was developed to enable the determination of enrofloxacin using photochemical derivatization which was used to both improve detection limits and to minimize the uncertainty of measurements. Phosphorescence was induced on cellulose containing TlNO(3). Absolute limit of detection at the ng range and linear analytical response over three orders of magnitude were achieved. A metrological study was made to obtain the combined uncertainty value and to identify that the precision was mainly affected by the changing of substrates when measuring the signal from each replicate. Pharmaceutical formulations containing enrofloxacin were successfully analyzed by the method and the results were similar to the ones achieved using a HPLC method. A solid phase extraction on an acrylic polymer was optimized to separate enrofloxacin from interferents such as diclofenac and other components from biological matrices, which allowed the successful use of the method in urine analysis.
authors.affiliation: Departamento de Química, Pontifícia Universidade Católica do Rio de Janeiro, Rio de Janeiro 22451-900, Brazil.
authors.forename: Cabrini F
authors.initials: CF
authors.lastname: de Souza
authors.forename: Renata K S
authors.initials: RK
authors.lastname: Martins
authors.forename: Andrea R
authors.initials: AR
authors.lastname: da Silva
authors.forename: Alessandra L M C
authors.initials: AL
authors.lastname: da Cunha
authors.forename: Ricardo Q
authors.initials: RQ
authors.lastname: Aucélio
mesh_terms: D000042:Absorption; D000179:Acrylates / Q000737:chemistry*; D002482:Cellulose / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004008:Diclofenac / Q000737:chemistry; D000077422:Enrofloxacin; D024841:Fluoroquinolones / Q000032:analysis* / Q000737:chemistry / Q000652:urine; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods*; D054802:Molecular Imprinting; D004364:Pharmaceutical Preparations / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods*; D012996:Solutions; D013050:Spectrometry, Fluorescence; D013696:Temperature
chemical_list: D000179:Acrylates; D024841:Fluoroquinolones; D004364:Pharmaceutical Preparations; D011108:Polymers; D012996:Solutions; D004008:Diclofenac; D000077422:Enrofloxacin; D002482:Cellulose; C036658:acrylic acid