title: Mosquitoes LTR retrotransposons: a deeper view into the genomic sequence of Culex quinquefasciatus.
abstract: A set of 67 novel LTR-retrotransposon has been identified by in silico analyses of the Culex quinquefasciatus genome using the LTR_STRUC program. The phylogenetic analysis shows that 29 novel and putatively functional LTR-retrotransposons detected belong to the Ty3/gypsy group. Our results demonstrate that, by considering only families containing potentially autonomous LTR-retrotransposons, they account for about 1% of the genome of C. quinquefasciatus. In previous studies it has been estimated that 29% of the genome of C. quinquefasciatus is occupied by mobile genetic elements.The potential role of retrotransposon insertions strictly associated with host genes is described and discussed along with the possible origin of a retrotransposon with peculiar Primer Binding Site region. Finally, we report the presence of a group of 38 retrotransposons, carrying tandem repeated sequences but lacking coding potential, and apparently lacking "master copy" elements from which they could have originated. The features of the repetitive sequences found in these non-autonomous LTR retrotransposons are described, and their possible role discussed.These results integrate the existing data on the genomics of an important virus-borne disease vector.
authors.affiliation: Dipartimento di Biologia - Sezione di Genetica e Microbiologia, Università di Bari Aldo Moro, Bari, Italy. r.marsano@biologia.uniba.it
authors.forename: Renè Massimiliano
authors.initials: RM
authors.lastname: Marsano
authors.forename: Daniela
authors.initials: D
authors.lastname: Leronni
authors.forename: Pietro
authors.initials: P
authors.lastname: D'Addabbo
authors.forename: Luigi
authors.initials: L
authors.lastname: Viggiano
authors.forename: Eustachio
authors.initials: E
authors.lastname: Tarasco
authors.forename: Ruggiero
authors.initials: R
authors.lastname: Caizzi
mesh_terms: D000465:Algorithms; D000818:Animals; D019295:Computational Biology / Q000379:methods; D003465:Culex / Q000235:genetics*; D009033:Culicidae / Q000235:genetics*; D019143:Evolution, Molecular; D016678:Genome; D023281:Genomics; D007303:Insect Vectors; D008957:Models, Genetic; D016366:Open Reading Frames; D010802:Phylogeny; D018626:Retroelements; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D020079:Terminal Repeat Sequences
chemical_list: D018626:Retroelements