title: CD19(+)CD1d(+)CD5(+) B cell frequencies are increased in patients with tuberculosis and suppress Th17 responses.
abstract: Although the importance of B cells in the host immune response upon Mycobacterium tuberculosis infection has been recognized, a conclusive role for B cells has yet to be determined. In the present study, we found that primary CD19(+) B cells isolated from patients with tuberculosis significantly inhibited Th17, but not Th1, cell activation. Moreover, the suppressive activity was mediated by a CD19(+)CD1d(+)CD5(+) B cell population. Notably, patients with tuberculosis were found to have significantly higher frequencies of CD19(+)CD1d(+)CD5(+) B cells with stronger suppressive activity than such cells from healthy donors. Furthermore, the frequency of CD19(+)CD1d(+)CD5(+) B cells in peripheral blood was inversely correlated with that of Th17 cells in patients with tuberculosis. This finding that B cells negatively regulate Th17 responses provides a novel mechanism in the regulation of CD4(+) T cell responses-aside from regulatory T cells-during M. tuberculosis infection, which may impact the clinical outcome of tuberculosis.
authors.affiliation: Shenzhen Key Laboratory for Diagnosis & Treatment of Infectious Diseases, Shenzhen 518020, China.
authors.forename: Mingxia
authors.initials: M
authors.lastname: Zhang
authors.forename: Xuebao
authors.initials: X
authors.lastname: Zheng
authors.forename: Jieyun
authors.initials: J
authors.lastname: Zhang
authors.forename: Yuzhen
authors.initials: Y
authors.lastname: Zhu
authors.forename: Xiuyun
authors.initials: X
authors.lastname: Zhu
authors.forename: Haiying
authors.initials: H
authors.lastname: Liu
authors.forename: Musheng
authors.initials: M
authors.lastname: Zeng
authors.forename: Michael W
authors.initials: MW
authors.lastname: Graner
authors.forename: Boping
authors.initials: B
authors.lastname: Zhou
authors.forename: Xinchun
authors.initials: X
authors.lastname: Chen
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D018941:Antigens, CD19 / Q000032:analysis; D055610:Antigens, CD1d / Q000032:analysis; D016175:B-Lymphocyte Subsets / Q000276:immunology*; D018956:CD5 Antigens / Q000032:analysis; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D006801:Humans; D008213:Lymphocyte Activation; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000276:immunology / Q000472:pathogenicity; D018417:Th1 Cells / Q000276:immunology; D058504:Th17 Cells / Q000276:immunology*; D014376:Tuberculosis / Q000276:immunology*; D055815:Young Adult
chemical_list: D018941:Antigens, CD19; D055610:Antigens, CD1d; D018956:CD5 Antigens; D016207:Cytokines