title: Signaling pathways of immobilized FGF-2 on silicon-substituted hydroxyapatite.
abstract: Therapeutic strategies for bone regeneration involve the selection of suitable biomaterials, growth factors, and cell types to mimic the cellular microenvironment where molecular and mechanical signals control the reconstruction of bone tissue. The immobilization of basic fibroblast growth factor (FGF-2) on powdered silicon-substituted hydroxyapatite (Si-HA) allows to prepare a biofunctional biomaterial able to interact with bone cells in a very specific way. The biological activity of FGF-2/Si-HA, evaluated in Saos-2 osteoblasts and MC3T3-E1 preosteoblasts through the PLCγ and MAPK/ERK signal transduction pathways, shows that FGF-2 immobilized on Si-HA provides the right signals to cells stimulating crucial intracellular mechanisms of osteoblast proliferation and differentiation.
authors.affiliation: Department of Biochemistry and Molecular Biology I, Faculty of Chemistry, Universidad Complutense, 28040 Madrid, Spain.
authors.forename: María
authors.initials: M
authors.lastname: de la Concepción Matesanz
authors.forename: María José
authors.initials: MJ
authors.lastname: Feito
authors.forename: Cecilia
authors.initials: C
authors.lastname: Ramírez-Santillán
authors.forename: Rosa María
authors.initials: RM
authors.lastname: Lozano
authors.forename: Sandra
authors.initials: S
authors.lastname: Sánchez-Salcedo
authors.forename: Daniel
authors.initials: D
authors.lastname: Arcos
authors.forename: María
authors.initials: M
authors.lastname: Vallet-Regí
authors.forename: María-Teresa
authors.initials: MT
authors.lastname: Portolés
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D040761:Biomimetic Materials / Q000138:chemical synthesis* / Q000494:pharmacology; D001842:Bone and Bones / Q000166:cytology / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D017886:Durapatite / Q000737:chemistry*; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D055767:Immobilized Proteins; D051379:Mice; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051966:Phospholipase C gamma / Q000378:metabolism; D011208:Powders; D015398:Signal Transduction / Q000502:physiology*; D012825:Silicon / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods
chemical_list: D055767:Immobilized Proteins; D011208:Powders; D016222:Fibroblast Growth Factor 2; D017886:Durapatite; D020929:Mitogen-Activated Protein Kinase Kinases; D051966:Phospholipase C gamma; D012825:Silicon