title: Cross-reactive antibodies to target proteins are dependent upon oligomannose glycosylated epitopes in HTLV-1 associated neurological disease.
abstract: Our lab recently identified a cross-reactive antibody response between human T-lymphotropic virus type-1-p24-(gag) (HTLV-1-p24-(gag)) and peroxiredoxin-1 (PrX-1) as potentially contributing to the pathogenesis of HTLV-1 associated neurological disease via molecular mimicry. These targets proteins were glycosylated, yet the glycan side chains immunoreactive with the immunoglobulins were unknown. Using a combination of lectin isolation and serial enzymatic deglycosylation of glycoproteins, we determined that the immunoreactive epitopes contained branched oligomannose side chains. These data suggest that post-translational glycosylation specifically related to oligomannose immunoreactivity to both the infecting and host antigens may contribute to molecular mimicry and be important in the pathogenesis of HTLV-1 associated neurological disease.
authors.affiliation: Research Service, Veterans Administration Medical Center, 1030 Jefferson Avenue, Memphis, TN 38104, USA.
authors.forename: Sangmin
authors.initials: S
authors.lastname: Lee
authors.forename: Yoojin
authors.initials: Y
authors.lastname: Shin
authors.forename: Daniel
authors.initials: D
authors.lastname: Clark
authors.forename: Eduardo
authors.initials: E
authors.lastname: Gotuzzo
authors.forename: Michael C
authors.initials: MC
authors.lastname: Levin
mesh_terms: D002460:Cell Line; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology; D006031:Glycosylation; D015368:Human T-lymphotropic virus 1 / Q000276:immunology* / Q000472:pathogenicity; D006801:Humans; D018716:Molecular Mimicry; D009422:Nervous System Diseases / Q000276:immunology* / Q000821:virology; D009844:Oligosaccharides / Q000276:immunology*; D015493:Paraparesis, Tropical Spastic / Q000276:immunology*; D054464:Peroxiredoxins / Q000276:immunology*; D011499:Protein Processing, Post-Translational; D015682:Retroviridae Proteins, Oncogenic / Q000276:immunology*
chemical_list: D000939:Epitopes; D009844:Oligosaccharides; D015682:Retroviridae Proteins, Oncogenic; C028569:oligomannoside; C060954:p24 protein, Human T-lymphotropic virus 1; C515940:PRDX1 protein, human; D054464:Peroxiredoxins