title: Enhancement of the activity and enantioselectivity of lipase by sol-gel encapsulation immobilization onto β-cyclodextrin-based polymer.
abstract: Candida rugosa lipase was encapsulated within a chemically inert sol-gel support prepared by polycondensation with tetraethoxysilane and octyltriethoxysilane in the presence of β-cyclodextrin-based polymer. The catalytic activity of the encapsulated lipases was evaluated both in the hydrolysis of p-nitrophenylpalmitate and the enantioselective hydrolysis of racemic Naproxen methyl ester. It has been observed that the percent activity yield of the encapsulated lipase was 65 U/g, which is 7.5 times higher than that of the covalently immobilized lipase. The β-cyclodextrin-based encapsulated lipases had higher conversion and enantioselectivity compared with covalently immobilized lipase. The study confirms an excellent enantioselectivity (E >300) for the encapsulated lipase with an enantiomeric excess value of 98% for S-naproxen.
authors.affiliation: Department of Chemistry, Faculty of Science, Selcuk University, Konya, Turkey. eyilmaz80@gmail.com
authors.forename: Elif
authors.initials: E
authors.lastname: Yilmaz
authors.forename: Mehmet
authors.initials: M
authors.lastname: Sezgin
mesh_terms: D002175:Candida / Q000201:enzymology; D002214:Capsules; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008049:Lipase / Q000737:chemistry* / Q000378:metabolism*; D009288:Naproxen / Q000031:analogs & derivatives / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D013237:Stereoisomerism; D013379:Substrate Specificity; D013696:Temperature; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D002214:Capsules; D004800:Enzymes, Immobilized; D011108:Polymers; D047392:beta-Cyclodextrins; C487872:naproxen methyl esther; D009288:Naproxen; D008049:Lipase