title: Amelioration of diet-induced nonalcoholic steatohepatitis in rats by Mn-salen complexes via reduction of oxidative stress.
abstract: BACKGROUND
  Nonalcoholic steatohepatitis (NASH), a progressive stage of nonalcoholic fatty liver disease (NAFLD), is characterized by steatosis (accumulation of triacylglycerols within hepatocytes) along with inflammation and ballooning degeneration. It has been suggested that oxidative stress may play an important role in the progress of NAFLD to NASH. The aim of present study was to determine whether antioxidant supplementations using EUK-8, EUK-134 and vitamin C could improve the biochemical and histological abnormalities associated with diet-induced NASH in rats.
  
  
  METHODS
  NASH was induced in male N-Mary rats by feeding a methionine - choline deficient (MCD) diet. The rats were fed either normal chow or MCD diet for 10 weeks. After NASH development, the MCD-fed rats were randomly divided into four groups of six: the NASH group that received MCD diet, the EUK-8 group which was fed MCD diet plus EUK-8, the EUK-134 group which was fed MCD diet plus EUK-134 and the vitamin C group which received MCD diet plus vitamin C. EUK-8, EUK-134 and vitamin C (30 mg/kg body weight/day) were administered by gavage for eight weeks.
  
  
  RESULTS
  Treatment of MCD-fed rats with salens reduced the sera aminotransferases, cholesterol, low density lipoprotein contents, the extent of lipid peroxidation and protein carbonylation whereas the HDL-C cholesterol levels were significantly increased. In addition, EUK-8 and EUK-134 improved steatosis, ballooning degeneration and inflammation in liver of MCD-fed rats.
  
  
  CONCLUSIONS
  Antioxidant (EUK-8, EUK-134 and vitamin C) supplementation reduces NASH-induced biochemical and histological abnormalities, pointing out that antioxidant strategy could be beneficial in treatment of NASH.
authors.affiliation: Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
authors.forename: Alireza
authors.initials: A
authors.lastname: Rezazadeh
authors.forename: Razieh
authors.initials: R
authors.lastname: Yazdanparast
authors.forename: Mahsa
authors.initials: M
authors.lastname: Molaei
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000008:administration & dosage*; D002796:Choline Deficiency; D004032:Diet; D004195:Disease Models, Animal; D005029:Ethylenediamines / Q000008:administration & dosage*; D005234:Fatty Liver / Q000139:chemically induced / Q000188:drug therapy*; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects*; D008715:Methionine / Q000172:deficiency; D009942:Organometallic Compounds / Q000008:administration & dosage*; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D012459:Salicylates / Q000008:administration & dosage*
chemical_list: C433928:EUK-134; D005029:Ethylenediamines; D009942:Organometallic Compounds; D012459:Salicylates; C090577:N,N'-bis(salicylideneamino)ethane-manganese(II); D008715:Methionine; D001205:Ascorbic Acid