title: Analysis of AGE modified proteins and RAGE expression in HER2/neu negative invasive ductal carcinoma.
abstract: Cancer is associated with increased glycolysis and carbonyl stress. In view of this, AGE modified proteins were identified from clinical breast cancer tissue using 2DE-immunoblot and mass-spectrometry. These proteins were identified to be serotransferrin, fibrinogen gamma chain, glycerol-3-phosphate dehydrogenase, lactate dehydrogenase, annexin II, prohibitin and peroxiredoxin 6, which have established role in cancer. Further, RAGE expression and its downstream signaling proteins NADPH oxidase and NF-kB were studied. Role of these AGE modified proteins and RAGE signaling in breast cancer is discussed.
authors.affiliation: Department of Chemistry, University of Pune, India.
authors.forename: Arvind M
authors.initials: AM
authors.lastname: Korwar
authors.forename: Hemangi S
authors.initials: HS
authors.lastname: Bhonsle
authors.forename: Ashok D
authors.initials: AD
authors.lastname: Chougale
authors.forename: Sachin S
authors.initials: SS
authors.lastname: Kote
authors.forename: Kachru R
authors.initials: KR
authors.lastname: Gawai
authors.forename: Vikram S
authors.initials: VS
authors.lastname: Ghole
authors.forename: Chaitanyananda B
authors.initials: CB
authors.lastname: Koppikar
authors.forename: Mahesh J
authors.initials: MJ
authors.lastname: Kulkarni
mesh_terms: D000595:Amino Acid Sequence; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D018270:Carcinoma, Ductal, Breast / Q000378:metabolism / Q000473:pathology*; D005260:Female; D017127:Glycation End Products, Advanced / Q000378:metabolism*; D006801:Humans; D020928:Mitogen-Activated Protein Kinases / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D019255:NADPH Oxidases / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D000067759:Receptor for Advanced Glycation End Products / Q000096:biosynthesis*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000032:analysis / Q000378:metabolism
chemical_list: D017127:Glycation End Products, Advanced; D020928:Mitogen-Activated Protein Kinases; D019255:NADPH Oxidases; D016328:NF-kappa B; D009363:Neoplasm Proteins; D000067759:Receptor for Advanced Glycation End Products; D018719:Erb-b2 Receptor Tyrosine Kinases