title: Cost-effective HLA-Cw06:02 typing in a Caucasian population.
abstract: Considering the clinical and genetic heterogeneity of psoriasis which may translate into distinct disease pathology and treatment response, correct typing of the main candidate gene HLA-C is critical but not trivial. To facilitate genotyping, we compared established techniques with our newly developed tool. Here, we propose that typing of four single nucleotide polymorphic markers within the HLA-C region correctly determines HLA-Cw*06:02 genotypes in psoriatic cases and healthy controls in a population of Caucasian origin. Typing of the SNPs presented herein proved to be precise, reliable, time and cost effective, and requiring low amount of DNA.
authors.forename: Pernilla
authors.initials: P
authors.lastname: Nikamo
authors.forename: Mona
authors.initials: M
authors.lastname: Ståhle
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D003362:Cost-Benefit Analysis; D005820:Genetic Testing / Q000191:economics* / Q000379:methods; D005838:Genotype; D015236:HLA-C Antigens / Q000235:genetics*; D006801:Humans; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D011565:Psoriasis / Q000208:ethnology / Q000235:genetics*; D015203:Reproducibility of Results; D044465:White People / Q000235:genetics*
chemical_list: D015236:HLA-C Antigens; C528994:HLA-C*06 antigen