title: Analysis of laser-induced heating in optical neuronal guidance.
abstract: Recently, it has been shown that it is possible to control the growth direction of neuronal growth cones by stimulation with weak laser light; an effect dubbed optical neuronal guidance. The effect exists for a broad range of laser wavelengths, spot sizes, spot intensities, optical intensity profiles and beam modulations, but it is unknown which biophysical mechanisms govern it. Based on thermodynamic modeling and simulation using published experimental parameters as input, we argue that the guidance is linked to heating. Until now, temperature effects due to laser-induced heating of the guided neuron have been neglected in the optical neuronal guidance literature. The results of our finite-element-method simulations show the relevance of the temperature field in optical guidance experiments and are consistent with published experimental results and modeling in the field of optical traps. Furthermore, we propose two experiments designed to test this hypotheses experimentally. For one of these experiments, we have designed a microfluidic platform, to be made using standard microfabrication techniques, for incubation of neurons in temperature gradients on micrometer lengthscales.
authors.affiliation: Niels Bohr Institute, University of Copenhagen, Blegdamsvej 17, DK-2100 Copenhagen Ø, Denmark. laut@fys.ku.dk
authors.forename: Christian L
authors.initials: CL
authors.lastname: Ebbesen
authors.forename: Henrik
authors.initials: H
authors.lastname: Bruus
mesh_terms: D000818:Animals; D020342:Finite Element Analysis; D020439:Growth Cones / Q000528:radiation effects*; D006358:Hot Temperature; D007834:Lasers; D051379:Mice; D046210:Microfluidic Analytical Techniques; D052898:Optical Tweezers; D051381:Rats