title: miR-21 and miR-101 regulate PLAP-1 expression in periodontal ligament             cells.
abstract: Periodontal ligament-associated protein-1 (PLAP-1/asporin) is a special             marker in periodontal ligament tissue. It is an important regulator of osteogenic             differentiation of periodontal ligament cells (PDLCs). This marker is also a prerequisite             for periodontal ligament development and mineralization in maintaining homeostasis             of the periodontium. However, the molecular mechanisms of the regulation of PLAP-1             expression at the post-transcriptional level remain unknown. By contrast, microRNAs             (miRNAs) provide an additional level of regulation beyond that of transcription             factors via regulation of the post-transcriptional control of gene expression.             This study was designed to analyze miRNA differential expression patterns of PDLCs             at various osteoblastic differentiation stages and to determine the contribution             of miRNAs in the regulation of PLAP-1 expression during osteoblast differentiation.             Bioinformatic analysis was performed to predict miRNAs that potentially regulate             the gene expression of PLAP-1. Dual luciferase reporter assay and qRT-PCR were             performed to confirm the effects of these miRNAs on PLAP-1 gene expression. Our             results indicated that mir-101 and mir-21 target PLAP-1 to regulate its expression             during osteogenic differentiation of PDLCs.
authors.affiliation: Department of Stomatology, Nanfang Hospital, Southern Medical University, College of Stomatology, Southern Medical University, Guangzhou, PR China.
authors.forename: Changxia
authors.initials: C
authors.lastname: Li
authors.forename: Chunlei
authors.initials: C
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Yue
authors.forename: Xin
authors.initials: X
authors.lastname: Huang
authors.forename: Meimei
authors.initials: M
authors.lastname: Chen
authors.forename: Jie
authors.initials: J
authors.lastname: Gao
authors.forename: Buling
authors.initials: B
authors.lastname: Wu
mesh_terms: D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis*; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis*; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D010513:Periodontal Ligament / Q000166:cytology / Q000378:metabolism*
chemical_list: C439889:ASPN protein, human; D016326:Extracellular Matrix Proteins; C534072:MIRN101 microRNA, human; C524940:MIRN21 microRNA, human; D035683:MicroRNAs