title: [Anti-inflammation effect of danggui shaoyao san on Alzheimer's diseases].
abstract: OBJECTIVE
  To study the mechanisms of Danggui Shaoyao San (DSS) on Alzheimer's diseases (AD) focusing on anti-inflammation.
  
  
  METHODS
  AD rats were established by intrahippocampal bilateral injection of Abeta1-42 protein. The AD rats were randomly divided into three groups: AD model group, DSS high-dose group, DSS low-dose group. Vehicle group rats were intrahippocampal bilateral injection of solvent with the same dose. The learning ability and memory of rats was investigated in step-down passive avoidance test and Morris water maze test, expression of IL-1beta, IL-6, TNF-alpha mRNA were observed by reverse transcriptase PCR (RT-PCR), levels of NO was measured by colorimetric method and neuron apoptosis in the hippocampus was investigated by tag method of TdT-mediated end-labeling of fragmented DNA (TUNEL).
  
  
  RESULTS
  DSS significantly reduced the escape latency and increased the time that rats spent in the target quadrant in Morris water maze test, shortened the responsive latency and decreased the error numbers in step-down passive avoidance test, reduced the expression of the IL-1beta, IL-6, TNF-alpha mRNA, and the level of the NO depressed the neuron apoptosis in the hippocampus.
  
  
  CONCLUSIONS
  DSS improving cognition of the rats might be related to attenuate inflammatory reaction and reduce cell apoptosis in the hippocampus.
authors.affiliation: Department of Histology and Embryology, Wannan Medical College, Wuhu 241003, China. zhongshuzhi2006@sohu.com
authors.forename: Shuzhi
authors.initials: S
authors.lastname: Zhong
authors.forename: Shiping
authors.initials: S
authors.lastname: Ma
authors.forename: Zongyuan
authors.initials: Z
authors.lastname: Hong
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Jin
mesh_terms: D000544:Alzheimer Disease / Q000188:drug therapy*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D006624:Hippocampus / Q000187:drug effects / Q000473:pathology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000893:Anti-Inflammatory Agents; D004365:Drugs, Chinese Herbal; C531150:danggui-shaoyao-san