title: Promiscuous esterase activities of the C-C hydrolases from Dyella ginsengisoli.
abstract: A C-C hydrolase gene (bphD(LA-4)) from strain Dyella ginsengisoli LA-4 was cloned and expressed in Escherichia coli BL21 (DE3). BphD(LA-4) together with another hydrolase MfphA(LA-4), which derived from the same strain, possessed esterase activities. p-Nitrophenyl butyrate was the best substrate for both enzymes. BphD(LA-4) had high catalytic efficiency to p-nitrophenyl benzoate, whereas MfphA(LA-4) had no activity. Homology modeling and docking studies demonstrated that the proper hydrogen bond interaction was important for the reactivity of specific substrate.
authors.affiliation: State Key Laboratory of Fine Chemicals and Industrial Ecology and Environmental Engineering (Ministry of Education, China), School of Environmental Science and Technology, Dalian University of Technology, Dalian, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhou
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Qu
authors.forename: Chunlei
authors.initials: C
authors.lastname: Kong
authors.forename: Yingge
authors.initials: Y
authors.lastname: Wu
authors.forename: Kang
authors.initials: K
authors.lastname: Zhu
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.forename: Jiti
authors.initials: J
authors.lastname: Zhou
mesh_terms: D002087:Butyrates / Q000378:metabolism; D003001:Cloning, Molecular; D004795:Enzyme Stability; D004926:Escherichia coli / Q000235:genetics; D004950:Esterases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D015870:Gene Expression; D008958:Models, Molecular; D011487:Protein Conformation; D013379:Substrate Specificity; D013696:Temperature; D044166:Xanthomonadaceae / Q000201:enzymology* / Q000235:genetics
chemical_list: D002087:Butyrates; C033592:4-nitrophenyl butyrate; D004950:Esterases