title: The composition of the culture medium influences the β-1,3-glucan metabolism of Aspergillus fumigatus and the antifungal activity of inhibitors of β-1,3-glucan synthesis.
abstract: In vitro testing of Aspergillus fumigatus susceptibility to echinocandins has always been a challenge. Using a simple and quick colorimetric method to analyze the activity of inhibitors of β-1,3-glucan synthesis, we found that the composition of the culture medium significantly influences glucan synthesis and consequently the antifungal properties of inhibitors of β-1,3-glucan synthesis when they are tested alone or in combination with chitin synthase inhibitors.
authors.affiliation: Unité des Aspergillus and Unité de Chimie et Biocatalyse, Institut Pasteur, Paris, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Clavaud
authors.forename: Anne
authors.initials: A
authors.lastname: Beauvais
authors.forename: Lise
authors.initials: L
authors.lastname: Barbin
authors.forename: Hélène
authors.initials: H
authors.lastname: Munier-Lehmann
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Latgé
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D001232:Aspergillus fumigatus / Q000187:drug effects* / Q000378:metabolism*; D000077336:Caspofungin; D003470:Culture Media / Q000494:pharmacology*; D054714:Echinocandins / Q000494:pharmacology; D006027:Glycosides / Q000494:pharmacology; D055666:Lipopeptides; D008825:Miconazole / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D014315:Triterpenes / Q000494:pharmacology; D047071:beta-Glucans / Q000378:metabolism*
chemical_list: D000935:Antifungal Agents; D003470:Culture Media; D054714:Echinocandins; D006027:Glycosides; D055666:Lipopeptides; D014315:Triterpenes; D047071:beta-Glucans; C421868:enfumafungin; D008825:Miconazole; C033363:beta-1,3-glucan; D000077336:Caspofungin