title: Gene cloning and characterization of a novel highly organic solvent tolerant lipase from Proteus sp. SW1 and its application for biodiesel production.
abstract: Proteus sp. SW1 was found to produce an extracellular solvent tolerant lipase. The gene, lipA, encoding a bacterial lipase, was cloned from total Proteus sp. SW1 DNA. lipA was predicted to encode a 287 amino acid protein of 31.2 kDa belonging to the Group I proteobacterial lipases. Purified His-tagged LipA exhibited optimal activity at pH 10.0 and 55°C. It was highly stable in organic solvents retaining 112% of its activity in 100% isopropanol after 24 h, and exhibited more than 200% of its initial activity upon exposure to 60% acetone, ethanol, and hexane for 18 h. Biodiesel synthesis reactions, using a single step addition of 13% an acyl acceptor ethanol, showed that LipA was highly effective at converting palm oil into biodiesel.
authors.affiliation: Laboratory of Biotechnology, Chulabhorn Research Institute, Kamphaengphet 6 Road, Bangkok 10210, Thailand.
authors.forename: Wirongrong
authors.initials: W
authors.lastname: Whangsuk
authors.forename: Pareenart
authors.initials: P
authors.lastname: Sungkeeree
authors.forename: Sirinthra
authors.initials: S
authors.lastname: Thiengmag
authors.forename: Jarunee
authors.initials: J
authors.lastname: Kerdwong
authors.forename: Ratiboot
authors.initials: R
authors.lastname: Sallabhan
authors.forename: Skorn
authors.initials: S
authors.lastname: Mongkolsuk
authors.forename: Suvit
authors.initials: S
authors.lastname: Loprasert
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D056804:Biofuels / Q000382:microbiology*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003001:Cloning, Molecular; D004951:Esterification; D008401:Gas Chromatography-Mass Spectrometry; D015723:Gene Library; D006586:Hexanes / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000096:biosynthesis* / Q000235:genetics; D000073878:Palm Oil; D010938:Plant Oils / Q000378:metabolism; D010957:Plasmids; D011511:Proteus / Q000201:enzymology* / Q000235:genetics; D012336:RNA, Ribosomal, 16S; D017423:Sequence Analysis, RNA; D012997:Solvents / Q000737:chemistry; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D056804:Biofuels; D006586:Hexanes; D010938:Plant Oils; D012336:RNA, Ribosomal, 16S; D012997:Solvents; C026385:n-hexane; D000073878:Palm Oil; D008049:Lipase