title: [Function of transport H+-ATPases in plant cell plasma and vacuolar membranes of maize under salt stress conditions and effect of adaptogenic preparations].
abstract: Participations of electrogenic H+-pumps of plasma and vacuolar membranes represented by E1-E2 and V-type H+-ATPases in plant cell adaptation to salt stress conditions has been studied by determination of their transport activities. Experiments were carried out on corn seedlings exposed during 1 or 10 days at 0.1 M NaCl. Preparations Methyure and Ivine were used by seed soaking at 10(-7) M. Plasma and vacuolar membrane fractions were isolated from corn seedling roots. In variants without NaCl a hydrolytical activity of plasma membrane H+-ATPase was increased with seedling age and its transport one was changed insignificantly, wherease the response of the weaker vacuolar H+-ATPase was opposite. NaCl exposition decreased hydrolytical activities of both H+-ATPases and increased their transport ones. These results demonstrated amplification of H+-pumps function especially represented by vacuolar H+-ATPase. Both preparations, Methyure mainly, caused a further increase of transport activity which was more expressed in NaCl variants. Obtained results showed the important role of these H+-pumps in plant adaptation under salt stress conditions realized by energetical maintenance of the secondary active Na+/H+ -antiporters which remove Na+ from cytoplasm.
authors.forename: Zh I
authors.initials: ZhI
authors.lastname: Rybchenko
authors.forename: T O
authors.initials: TO
authors.lastname: Palladina
mesh_terms: D000222:Adaptation, Physiological / Q000502:physiology; D002462:Cell Membrane / Q000378:metabolism; D059828:Plant Cells / Q000201:enzymology*; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D054712:Salinity; D012965:Sodium Chloride / Q000494:pharmacology; D025262:Vacuolar Proton-Translocating ATPases / Q000378:metabolism; D014617:Vacuoles / Q000378:metabolism; D003313:Zea mays / Q000201:enzymology* / Q000378:metabolism
chemical_list: D012965:Sodium Chloride; D025262:Vacuolar Proton-Translocating ATPases; D006180:Proton-Translocating ATPases