title: HIV-1 inhibits phagocytosis and inflammatory cytokine responses of human monocyte-derived macrophages to P. falciparum infected erythrocytes.
abstract: HIV-1 infection increases the risk and severity of malaria by poorly defined mechanisms. We investigated the effect of HIV-1(Ba-L) infection of monocyte-derived macrophages (MDM) on phagocytosis of opsonised P. falciparum infected erythrocytes (IE) and subsequent proinflammatory cytokine secretion. Compared to mock-infected MDM, HIV-1 infection significantly inhibited phagocytosis of IE (median (IQR) (10 (0-28) versus (34 (27-108); IE internalised/100 MDM; p = 0.001) and decreased secretion of IL-6 (1,116 (352-3,387) versus 1,552 (889-6,331); pg/mL; p = 0.0078) and IL-1β (16 (7-21) versus 33 (27-65); pg/mL; p = 0.0078). Thus inadequate phagocytosis and cytokine production may contribute to impaired control of malaria in HIV-1 infected individuals.
authors.affiliation: Department of Medicine (RMH), University of Melbourne, Post Office Royal Melbourne Hospital, Melbourne, Victoria, Australia. lludlow@unimelb.edu.au
authors.forename: Louise E
authors.initials: LE
authors.lastname: Ludlow
authors.forename: Jingling
authors.initials: J
authors.lastname: Zhou
authors.forename: Emma
authors.initials: E
authors.lastname: Tippett
authors.forename: Wan-Jung
authors.initials: WJ
authors.lastname: Cheng
authors.forename: Wina
authors.initials: W
authors.lastname: Hasang
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Rogerson
authors.forename: Anthony
authors.initials: A
authors.lastname: Jaworowski
mesh_terms: D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D004912:Erythrocytes / Q000469:parasitology*; D005786:Gene Expression Regulation; D015658:HIV Infections / Q000150:complications / Q000821:virology; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism*; D008264:Macrophages / Q000166:cytology / Q000276:immunology / Q000821:virology*; D016778:Malaria, Falciparum / Q000150:complications / Q000469:parasitology; D009895:Opsonin Proteins / Q000276:immunology; D010587:Phagocytosis; D010963:Plasmodium falciparum / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013997:Time Factors
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D009895:Opsonin Proteins; D012333:RNA, Messenger