title: Culture of rodent cortical and hippocampal neurons.
abstract: Neurons cultured from rodent central nervous system tissues represent an important tool in the study of neurodegenerative disease mechanisms and neuroregenerative processes, including the survival- and axon growth-promoting properties of neurotrophic factors. This chapter presents a detailed protocol for the preparation of rat and mouse cortical and hippocampal neuron cell cultures using either embryonic or postnatal tissue with enzymatic digestion.
authors.affiliation: Department of Pharmacology and Anesthesiology, University of Padova, Padova, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Facci
authors.forename: Stephen D
authors.initials: SD
authors.lastname: Skaper
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation / Q000379:methods*; D002540:Cerebral Cortex / Q000166:cytology*; D004210:Dissection / Q000379:methods; D004622:Embryo, Mammalian / Q000166:cytology; D006624:Hippocampus / Q000166:cytology*; D051379:Mice; D009474:Neurons / Q000166:cytology*; D010206:Papain; D051381:Rats
chemical_list: D010206:Papain