title: C-Terminal region of 48-kDa rhoptry protein for serological detection of Babesia caballi antibodies in horses.
abstract: A recombinant C-terminal antigen derived from Babesia caballi 48-kDa rhoptry protein (rBc48/CT) was made for the development of a serologically diagnostic test. Antiserum raised against the rBc48/CT reacted specifically with the corresponding native protein by Western blotting and the indirect fluorescent antibody test (IFAT). Next, an indirect enzyme-linked immunosorbent assay (Bc48/CT-ELISA) and an immunochromatographic test based on the Bc48/CT (Bc48/CT-ICT) were constructed and employed for the detection of an antibody to B. caballi in a variety of equine sera. The results of Bc48/CT-ELISA and Bc48/CT-ICT were highly concordant with those of IFAT and ELISA, with full-length protein of Bc48 used as the reference tests. Our results demonstrate the success of Bc48/CT as antigen for the serological diagnosis of B. caballi infection in horses.
authors.affiliation: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
authors.forename: Mohamad Alaa
authors.initials: MA
authors.lastname: Terkawi
authors.forename: Hend
authors.initials: H
authors.lastname: Alhasan
authors.forename: Akio
authors.initials: A
authors.lastname: Ueno
authors.forename: Jadsada
authors.initials: J
authors.lastname: Ratthanophart
authors.forename: Yuzi
authors.initials: Y
authors.lastname: Luo
authors.forename: Shinuo
authors.initials: S
authors.lastname: Cao
authors.forename: Ketsarin
authors.initials: K
authors.lastname: Kamyingkird
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Aboulaila
authors.forename: Goo
authors.initials: G
authors.lastname: Youn-Kyoung
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Nishikawa
authors.forename: Naoaki
authors.initials: N
authors.lastname: Yokoyama
authors.forename: Xuenan
authors.initials: X
authors.lastname: Xuan
authors.forename: Ikuo
authors.initials: I
authors.lastname: Igarashi
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood* / Q000276:immunology; D000953:Antigens, Protozoan / Q000276:immunology; D001403:Babesia / Q000254:growth & development / Q000276:immunology* / Q000302:isolation & purification; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005455:Fluorescent Antibody Technique / Q000379:methods; D006736:Horses / Q000276:immunology / Q000469:parasitology*; D007106:Immune Sera / Q000276:immunology; D015800:Protozoan Proteins / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D007106:Immune Sera; D015800:Protozoan Proteins; D011994:Recombinant Proteins; C097551:rhoptry-associated protein-1, Babesia