title: Regulation of CD44 expression by tumor necrosis factor-α and its potential role in breast cancer cell migration.
abstract: CD44 molecule plays critical role in distant malignant metastasis. It is expressed in standard form (CD44s) or variant form (CD44v). Tumor necrosis factor-α (TNF-α) is highly expressed in the cancer microenvironment. TNF-α was reported to modulate CD44 expression in several kinds of cancer. However, little is known about pathological role of TNF-α in breast cancer (BC) cells. In the current investigation, we investigated the effect of TNF-α on BC cells (MCF-7 and MDA-MB-231) viability, CD44 expression, and in vitro migration. We found that TNF-α down-regulated CD44s expression, up-regulated CD44v3 and CD44v6 expression through JNK pathway in MCF-7 cells. In MDA-MB-231 cells, TNF-α up-regulated CD44s, CD44v3 and CD44v6 expression via p38 pathway. These data indicate important role of CD44 molecule in BC pathology.
authors.affiliation: Department of Medical Oncology, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, 210029 Nanjing, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Zha
authors.forename: Rong
authors.initials: R
authors.lastname: Wang
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Li
authors.forename: Bei
authors.initials: B
authors.lastname: Xu
authors.forename: Yan-Jie
authors.initials: YJ
authors.lastname: Xu
authors.forename: Yong-Mei
authors.initials: YM
authors.lastname: Yin
mesh_terms: D001943:Breast Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology*; D002470:Cell Survival / Q000502:physiology; D015536:Down-Regulation / Q000502:physiology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006801:Humans; D018960:Hyaluronan Receptors / Q000235:genetics*; D020935:MAP Kinase Signaling System / Q000502:physiology; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology*; D015854:Up-Regulation / Q000502:physiology
chemical_list: C473647:CD44S antigen; C121450:CD44V3,8-10; C112697:CD44v6 antigen; D018960:Hyaluronan Receptors; D014409:Tumor Necrosis Factor-alpha