title: [Chemical constituents of endophytic fungus Nodulisporium sp. A4 from Aquilaria sinensis].
abstract: OBJECTIVE
  To investigate the chemical constituents of an endophytic fungus, Nodulisporium sp. A4, from the medicinal plant Aquilaria sinensis and search for antitumor natural products.
  
  
  METHODS
  The fungus was cultured in liquid medium and extracted with EtOAc. The compounds were isolated by various chromatographic methods (silica gel, reverse silica gel, Sephadex-LH20, preparative TLC and so on) and recrystallization. Structural elucidation was conducted by extensive analysis of spectroscopic data as well as by comparison with literature reports. The antitumor activity of isolated compounds was tested by MTT method in vitro.
  
  
  RESULTS
  Seven compounds were isolated and identified from the broth culture, their structures were determined to be 5-methyl-2-vinyltetrahydrofuran-3-ol (1), 6-methyl-2-(5-methyl-5-vinyltetrahydrofuran-2-yl) hept-5-en-2-ol (2), 6alpha-hydroxycyclonerolidol (3), rel-(1S,4S, 5R,7R,10R)-10-desmethyl-1-methyl-11-eudesmene (4), tyrosol (5), 8-methoxynaphthalen-1-ol (6), and 1,8-dimethoxynaphthalene (7). Three compounds were isolated and identified from the mycelia as ergosterol (8), ergosterol peroxide (9), and cerevisterol (10). The in vitro pharmalogical evaluation results displayed that compounds 3 and 4 showed 89.1%, 44.2% and 82.3%, 79.8% inhibition against tumor cell lines SF268 and NCI-H460 at 100 mg x L(-1), respectively.
  
  
  CONCLUSIONS
  Compound 1 was a new natural product, compounds 2, 3, 7 and 10 were reported from the genus Nodulisporium sp. for the first time. Compounds 3 and 4 exhibited weak inhibitory effects on the proliferation of tumor cell lines SF268 and NCI-H460.
authors.affiliation: State Key Laboratory of Applied Microbiology Ministry-Guangdong Province Jointly Breeding Base, South China.
authors.forename: Dongli
authors.initials: D
authors.lastname: Li
authors.forename: Zhengchao
authors.initials: Z
authors.lastname: Wu
authors.forename: Yuchan
authors.initials: Y
authors.lastname: Chen
authors.forename: Meihua
authors.initials: M
authors.lastname: Tao
authors.forename: Weimin
authors.initials: W
authors.lastname: Zhang
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D060026:Endophytes / Q000737:chemistry*; D006801:Humans; D009930:Organic Chemicals / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D029645:Thymelaeaceae / Q000382:microbiology*; D013098:Xylariales / Q000737:chemistry*
chemical_list: D009930:Organic Chemicals