title: [Influence of liquid or solid culture conditions on the volatile components of mycelia of Isariacateinannulata].
abstract: OBJECTIVE
  To determine the volatile components of mycelia of Isaria cateinannulata cultured under different culture conditions, and to analyze the relationships between the culture conditions and volatile metabolites.
  
  
  METHODS
  Mycelia were cultured in solid plates with SDAY medium and liquid shake flasks with SDY medium. The culture conditions were at 25 degrees C and 8 days. Volatile components in the mycelia of I. cateinannulata were extracted with simultaneous distillation extraction and analyzed by gas chromatography-mass spectrometry.
  
  
  RESULTS
  Alkenes, alkanes, heterocyclic and polycyclic aromatic hydrocarbons (PAH) were existed abundantly both in the mycelia of liquid and solid cultures, but the kinds and relative concentrations of the volatile components in mycelia of liquid and solid cultures were very different. Forty-one compounds were identified from the mycelia of solid culture and 32 compounds were identified from the mycelia of liquid culture. Esters, quinones and oximes were only found in solid cultured mycelia whereas carboxylic acids were only discovered in the mycelia of liquid culture. At the same time, mycelia of liquid culture contained much more phenols. The most abundant compounds in mycelia of liquid and solid cultures were hydrocarbons. The volatile extracts of solid cultured mycelia contained 57.6% alkenes and 9.19% alkanes. The volatile extracts of liquid cultured mycelia contained 7.85% alkenes and 22.4% alkanes.
  
  
  CONCLUSIONS
  Liquid or solid culture conditions influenced the volatile components of mycelia of I. cateinannulata.
authors.affiliation: Anhui Provincial Key Laboratory of Microbial Control, Anhui Agricultural University, Hefei 230036, China.
authors.forename: Delong
authors.initials: D
authors.lastname: Zhang
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Wang
authors.forename: Ruili
authors.initials: R
authors.lastname: Lu
authors.forename: Kangle
authors.initials: K
authors.lastname: Li
authors.forename: Fenglin
authors.initials: F
authors.lastname: Hu
mesh_terms: D003470:Culture Media / Q000737:chemistry / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D006999:Hypocreales / Q000737:chemistry / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D025282:Mycelium / Q000737:chemistry / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D055549:Volatile Organic Compounds / Q000737:chemistry / Q000378:metabolism*
chemical_list: D003470:Culture Media; D055549:Volatile Organic Compounds