title: Competitive cellular uptake of nanoparticles made from polystyrene, poly(methyl methacrylate), and polylactide.
abstract: The uptake behavior of negatively charged fluorescent nanoparticles made from different polymers (PS, PMMA, and PLLA) is studied on HeLa cells. All particles are obtained by the miniemulsion process using sodium dodecylsulfate as anionic surfactant. The size of the particles is in the range 105-125 nm. Cell uptake is analyzed by flow cytometry and reveals a higher uptake of PLLA particles compared to PMMA and PS particles. In competitive uptake studies two different types of particles are co-incubated with the HeLa cells; the results indicate a mutual influence of the particles on their uptake behavior. A reduced internalization of PLLA particles in the presence of PS particles is observed, although neither the co-incubation of PMMA and PLLA nor of PMMA and PS shows similar effect.
authors.affiliation: Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany.
authors.forename: Anita
authors.initials: A
authors.lastname: Höcherl
authors.forename: Martin
authors.initials: M
authors.lastname: Dass
authors.forename: Katharina
authors.initials: K
authors.lastname: Landfester
authors.forename: Volker
authors.initials: V
authors.lastname: Mailänder
authors.forename: Anna
authors.initials: A
authors.lastname: Musyanovych
mesh_terms: D001798:Blood Proteins / Q000494:pharmacology; D003470:Culture Media; D004337:Drug Carriers; D004655:Emulsions / Q000737:chemistry; D004705:Endocytosis / Q000187:drug effects*; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011091:Polyesters / Q000138:chemical synthesis / Q000494:pharmacology*; D019904:Polymethyl Methacrylate / Q000138:chemical synthesis / Q000494:pharmacology*; D011137:Polystyrenes / Q000138:chemical synthesis / Q000494:pharmacology*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D055672:Static Electricity; D013501:Surface-Active Agents / Q000737:chemistry
chemical_list: D001798:Blood Proteins; D003470:Culture Media; D004337:Drug Carriers; D004655:Emulsions; D005456:Fluorescent Dyes; D011091:Polyesters; D011137:Polystyrenes; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; C033616:poly(lactide); D019904:Polymethyl Methacrylate