title: Preparation and characterization of malonic acid cross-linked chitosan and collagen 3D scaffolds: an approach on non-covalent interactions.
abstract: The present study emphasizes the influence of non-covalent interactions on the mechanical and thermal properties of the scaffolds of chitosan/collagen origin. Malonic acid (MA), a bifuncitonal diacid was chosen to offer non-covalent cross-linking. Three dimensional scaffolds was prepared using chitosan at 1.0% (w/v) and MA at 0.2% (w/v), similarly collagen 0.5% (w/v) and MA 0.2% (w/v) and characterized. Results on FT-IR, TGA, DSC, SEM and mechanical properties (tensile strength, stiffness, Young's modulus, etc.) assessment demonstrated the existence of non-covalent interaction between MA and chitosan/collagen, which offered flexibility and high strength to the scaffolds suitable for tissue engineering research. Studies using NIH 3T3 fibroblast cells suggested biocompatibility nature of the scaffolds. Docking simulation study further supports the intermolecular hydrogen bonding interactions between MA and chitosan/collagen.
authors.affiliation: Microbiology Division, Central Leather Research Institute (CSIR, New Delhi), Adyar, Chennai 600020, Tamil Nadu, India.
authors.forename: Tapas
authors.initials: T
authors.lastname: Mitra
authors.forename: G
authors.initials: G
authors.lastname: Sailakshmi
authors.forename: A
authors.initials: A
authors.lastname: Gnanamani
authors.forename: A B
authors.initials: AB
authors.lastname: Mandal
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000138:chemical synthesis* / Q000737:chemistry; D003094:Collagen / Q000138:chemical synthesis* / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000494:pharmacology; D006860:Hydrogen Bonding; D008314:Malonates / Q000737:chemistry / Q000494:pharmacology*; D008422:Materials Testing; D051379:Mice; D008954:Models, Biological; D008958:Models, Molecular; D008968:Molecular Conformation; D041681:NIH 3T3 Cells; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D003432:Cross-Linking Reagents; D008314:Malonates; D003094:Collagen; D048271:Chitosan; C030290:malonic acid