title: Multiple-locus variable number of tandem repeats fingerprinting (MLVF) and virulence factor analysis of methicillin resistant Staphylococcus aureus SCCmec type III.
abstract: Methicillin resistant Staphylococcus aureus (MRSA), particularly strains with type III staphylococcal cassette chromosome mec (SCCmec), represent a serious human pathogen in Tehran, Iran. The disease-causing capability depends on their ability to produce a wide variety of virulent factors. The prevalence of exotoxin genes and multiple-locus variable number of tandem repeats fingerprinting (MLVF) profile among MRSA isolates, from patients in Tehran, was evaluated by PCR and Multiplex-PCR. The MLVF typing of 144 MRSA isolates with type III SCCmec produced 5 different MLVF types. Generally, 97.2% (140/144) of all the isolates were positive for at least one of the tested exotoxin genes. The most prevalent genes were hld, found in 87.5% (126/144) of the isolates followed by lukE-lukD and hla found in 72.9% (105/144) and 70.1% (101/144) of the isolates, respectively. The tst gene, belonging to MLVF types I, IV and V, was found among three of the isolates from blood and wound samples. The sea gene was detected in 58.3% (84/144) of the isolates and the sed and see genes were found in one isolate with MLVF type V. The coexistence of genes was observed in the 87.5% (126/144) of the isolates. The rate of coexistence of hld with lukE-lukD, hla with lukE-lukD and sea with lukE-lukD were 66.7% (96/144), 44.4% (64/144) and 44.4% (64/144), respectively. The present study demonstrated that MRSA strains with type III SCCmec show different MLVF patterns and exotoxin profiles.
authors.affiliation: Department of Microbiology, School of Medicine, Tehran University of Medical Sciences. Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Emaneini
authors.forename: Leila
authors.initials: L
authors.lastname: Jabalameli
authors.forename: Hossein
authors.initials: H
authors.lastname: Iman-Eini
authors.forename: Marzieh
authors.initials: M
authors.lastname: Aligholi
authors.forename: Amir
authors.initials: A
authors.lastname: Ghasemi
authors.forename: Farrokh Akbari
authors.initials: FA
authors.lastname: Nakhjavani
authors.forename: Morovat
authors.initials: M
authors.lastname: Taherikalani
authors.forename: Babak
authors.initials: B
authors.lastname: Khoramian
authors.forename: Parisa
authors.initials: P
authors.lastname: Asadollahi
authors.forename: Fereshteh
authors.initials: F
authors.lastname: Jabalameli
mesh_terms: D016172:DNA Fingerprinting / Q000379:methods; D005098:Exotoxins / Q000235:genetics; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity*; D018598:Minisatellite Repeats / Q000235:genetics*; D058889:Molecular Typing / Q000379:methods; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D005098:Exotoxins; D037521:Virulence Factors