title: Genome-wide association study identifies novel loci associated with serum level of vitamin B12 in Chinese men.
abstract: Vitamin B12 (VitB12 or cobalamin) is an essential cofactor in several metabolic pathways. Clinically, VitB12 deficiency is associated with pernicious anemia, neurodegenerative disorder, cardiovascular disease and gastrointestinal disease. Although previous genome-wide association studies (GWAS) identified several genes, including FUT2, CUBN, TCN1 and MUT, that may influence VitB12 levels in European populations, common genetic determinants of VitB12 remain largely unknown, especially in Asian populations. Here we performed a GWAS in 1999 healthy Chinese men and replicated the top findings in an independent Chinese sample with 1496 subjects. We identified four novel genomic loci that were significantly associated with serum level of VitB12 at a genome-wide significance level of 5.00 × 10(-8). These four loci were MS4A3 (11q12.1; rs2298585; P= 2.64 × 10(-15)), CLYBL (13q32; rs41281112; P= 9.23 × 10(-10)), FUT6 (19p13.3; rs3760776; P= 3.68 × 10(-13)) and 5q32 region (rs10515552; P= 3.94 × 10(-8)). In addition, we also confirmed the association with the serum level of VitB12 for the previously reported FUT2 gene and identified one novel non-synonymous single-nucleotide polymorphism in FUT2 gene in this Chinese population (19q13.33; rs1047781; P= 3.62 × 10(-36)). The new loci identified offer new insights into the biochemical pathways involved in determining the serum level of VitB12 and provide opportunities to better delineate the role of VitB12 in health and disease.
authors.affiliation: Fudan Institute of Urology, Huashan Hospital, School of Life Sciences, Fudan University, Shanghai, China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Lin
authors.forename: Daru
authors.initials: D
authors.lastname: Lu
authors.forename: Yong
authors.initials: Y
authors.lastname: Gao
authors.forename: Sha
authors.initials: S
authors.lastname: Tao
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Yang
authors.forename: Junjie
authors.initials: J
authors.lastname: Feng
authors.forename: Aihua
authors.initials: A
authors.lastname: Tan
authors.forename: Haiying
authors.initials: H
authors.lastname: Zhang
authors.forename: Yanling
authors.initials: Y
authors.lastname: Hu
authors.forename: Xue
authors.initials: X
authors.lastname: Qin
authors.forename: Seong-Tae
authors.initials: ST
authors.lastname: Kim
authors.forename: Tao
authors.initials: T
authors.lastname: Peng
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Linjian
authors.initials: L
authors.lastname: Mo
authors.forename: Shijun
authors.initials: S
authors.lastname: Zhang
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Trent
authors.forename: Zengnan
authors.initials: Z
authors.lastname: Mo
authors.forename: S Lilly
authors.initials: SL
authors.lastname: Zheng
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Xu
authors.forename: Jielin
authors.initials: J
authors.lastname: Sun
mesh_terms: D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics*; D056426:Genetic Loci; D020022:Genetic Predisposition to Disease; D055106:Genome-Wide Association Study; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D014805:Vitamin B 12 / Q000097:blood* / Q000235:genetics* / Q000378:metabolism
chemical_list: D014805:Vitamin B 12