title: Engineered polymer-supported synthesis of 3'-carboxyalkyl-modified oligonucleotides and their applications in the construction of biochips for diagnosis of the diseases.
abstract: An engineered polymer support 5 has been prepared for the solid-phase assembly of 3'-carboxyalkyl-modified oligonucleotides using commonly available reagents. A two-step deprotection procedure resulted in the quantitative cleavage of oligonucleotides from the support and removal of the protecting groups from phosphodiesters and exocyclic amino groups of the nucleic bases. The fully deprotected oligomers, obtained in high yield, were desalted and analyzed on RP-HPLC. After characterization by MALDI-TOF, these carboxyalkylated oligonucleotides were immobilized onto the epoxy-functionalized glass microslides to prepare biochips. The performance of these biochips was evaluated under different sets of conditions and then successfully validated by the detection of base mismatches and human infectious disease, bacterial meningitis, caused by N. meningitidis.
authors.affiliation: CSIR-Institute of Genomics and Integrative Biology, Delhi University Campus, Mall Road, Delhi 110 007, India.
authors.forename: Satyakam
authors.initials: S
authors.lastname: Patnaik
authors.forename: Sandip K
authors.initials: SK
authors.lastname: Dash
authors.forename: Dalip
authors.initials: D
authors.lastname: Sethi
authors.forename: Ashok
authors.initials: A
authors.lastname: Kumar
authors.forename: K C
authors.initials: KC
authors.lastname: Gupta
authors.forename: Pradeep
authors.initials: P
authors.lastname: Kumar
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006801:Humans; D009841:Oligonucleotides / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D011108:Polymers / Q000737:chemistry*; D040081:Protein Array Analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D009841:Oligonucleotides; D011108:Polymers