title: Genetic relationship of cowpea (Vigna unguiculata)  varieties from Senegal based on SSR markers.
abstract: Genetic diversity and phylogenetic relationships among  22 local cowpea (Vigna unguiculata) varieties and inbred lines collected  throughout Senegal were evaluated using simple sequence repeat molecular  markers. A set of 49 primer combinations were developed from cowpea  genomic/expressed sequence tags and evaluated for their ability to detect  polymorphisms among the various cowpea genotypes. Forty-four primer  combinations detected polymorphisms, with the remaining five primer sets  failing to yield PCR amplification products. From one to 16 alleles were found  among the informative primer combinations; their frequencies ranged from 0.60  to 0.95 (mean = 0.79). The genetic diversity of the sample varied from 0.08 to  0.42 (mean = 0.28). The polymorphic information content ranged from 0.08 to  0.33 (mean = 0.23). The local varieties clustered in the same group, except  53-3, 58-53, and 58-57; while Ndoute yellow pods, Ndoute violet pods and Baye  Ngagne were in the second group. The photosensitive varieties (Ndoute yellow  pods and Ndoute violet pods) were closely clustered in the second group and so  were inbred line Mouride and local cultivar 58-57, which is also one of the  parents for inbred line Mouride. These molecular markers could be used for  selection and identification of elite varieties for cowpea improvement and  germplasm management in Senegal.
authors.affiliation: Plant Biotechnology Laboratory, Department of Plant Biology, Faculty of Science and Technology, University Cheikh Anta Diop, Dakar, Senegal.
authors.forename: F A
authors.initials: FA
authors.lastname: Badiane
authors.forename: B S
authors.initials: BS
authors.lastname: Gowda
authors.forename: N
authors.initials: N
authors.lastname: Cissé
authors.forename: D
authors.initials: D
authors.lastname: Diouf
authors.forename: O
authors.initials: O
authors.lastname: Sadio
authors.forename: M P
authors.initials: MP
authors.lastname: Timko
mesh_terms: D000483:Alleles; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D007887:Fabaceae / Q000145:classification* / Q000235:genetics*; D014644:Genetic Variation; D018895:Microsatellite Repeats / Q000235:genetics*; D010802:Phylogeny; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D012675:Senegal
chemical_list: D017931:DNA Primers; D018744:DNA, Plant