title: Preparation and characterization of biocompatible chitosan nanoparticles for targeted brain delivery of peptides.
abstract: Here, we describe a nanocarrier system that can transfer chitosan nanoparticles loaded with either small peptides such as the caspase inhibitor Z-DEVD-FMK or a large peptide like basic fibroblast growth factor across the blood-brain barrier. The nanoparticles are selectively directed to the brain and are not measurably taken up by liver and spleen. Intravital fluorescent microscopy provides an opportunity to study the penetration kinetics of nanoparticles loaded with fluorescent agents such as Nile red, and has demonstrated that this nanomedicine formulation is rapidly transported across the blood-brain barrier.
authors.affiliation: Department of Pharmaceutical Technology, Hacettepe University, Ankara, Turkey.
authors.forename: Secil
authors.initials: S
authors.lastname: Caban
authors.forename: Yılmaz
authors.initials: Y
authors.lastname: Capan
authors.forename: Patrick
authors.initials: P
authors.lastname: Couvreur
authors.forename: Turgay
authors.initials: T
authors.lastname: Dalkara
mesh_terms: D000911:Antibodies, Monoclonal; D001672:Biocompatible Materials / Q000737:chemistry*; D001812:Blood-Brain Barrier / Q000378:metabolism; D048271:Chitosan / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000379:methods*; D005346:Fibroblast Growth Factors; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D009842:Oligopeptides; D010455:Peptides / Q000008:administration & dosage; D019809:Streptavidin
chemical_list: D000911:Antibodies, Monoclonal; D001672:Biocompatible Materials; D009842:Oligopeptides; D010455:Peptides; C110772:benzoylcarbonyl-aspartyl-glutamyl-valyl-aspartyl-fluoromethyl ketone; D005346:Fibroblast Growth Factors; D048271:Chitosan; D019809:Streptavidin