title: Immobilization of paraoxonase onto chitosan and its characterization.
abstract: Paraoxonase was covalently immobilized onto a glutaraldehyde containing amino group functionalized chitosan surface by chemical immobilization at pH 8.0. The amount of covalently bound hPON1 was found to be 32 mg/10 chitosan beads. The properties of immobilized enzyme were investigated and compared to those of free enzyme. The effects of various parameters such as pH, temperature, heat, and storage stability on immobilized enzyme were investigated. Kinetic parameters of the immobilized enzyme were also evaluated. Thermal and storage stability experiments were carried out. It was observed that the immobilized enzyme had longer storage stability and retained 50 % of its initial activity during 26 days.
authors.affiliation: Science and Art Faculty, Department of Chemistry/Biochemistry Section, Balikesir University, Balikesir, Turkey.
authors.forename: Utku
authors.initials: U
authors.lastname: Colak
authors.forename: Nahit
authors.initials: N
authors.lastname: Gençer
mesh_terms: D043303:Aryldialkylphosphatase / Q000097:blood / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D004355:Drug Stability; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000097:blood / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008863:Microspheres; D013696:Temperature
chemical_list: D004800:Enzymes, Immobilized; D048271:Chitosan; D043303:Aryldialkylphosphatase