title: Imaging P2X4 receptor lateral mobility in microglia: regulation by calcium and p38 MAPK.
abstract: ATP-gated ionotropic P2X4 receptors are up-regulated in activated microglia and are critical for the development of neuropathic pain, a microglia-associated disorder. However, the nature of how plasma membrane P2X4 receptors are regulated in microglia is not fully understood. We used single-molecule imaging to track quantum dot-labeled P2X4 receptors to explore P2X4 receptor mobility in the processes of resting and activated microglia. We find that plasma membrane P2X4 receptor lateral mobility in resting microglial processes is largely random, consisting of mobile and slowly mobile receptors. Moreover, lateral mobility is P2X subunit- and cell-specific, increased in an ATP activation and calcium-dependent manner, and enhanced in activated microglia by the p38 MAPK pathway that selectively regulates slowly mobile receptors. Thus, our data indicate that P2X4 receptors are dynamically regulated mobile ATP sensors, sampling more of the plasma membrane in response to ATP and during the activated state of microglia that is associated with nervous system dysfunction.
authors.affiliation: Department of Physiology, David Geffen School of Medicine, UCLA, Los Angeles, California 90095, USA.
authors.forename: Estelle
authors.initials: E
authors.lastname: Toulme
authors.forename: Baljit S
authors.initials: BS
authors.lastname: Khakh
mesh_terms: D000255:Adenosine Triphosphate / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D002462:Cell Membrane / Q000235:genetics / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D020935:MAP Kinase Signaling System; D017628:Microglia / Q000378:metabolism*; D009437:Neuralgia / Q000235:genetics / Q000378:metabolism; D021381:Protein Transport / Q000235:genetics; D045663:Quantum Dots; D051381:Rats; D058848:Receptors, Purinergic P2X4 / Q000235:genetics / Q000378:metabolism*; D048051:p38 Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism*
chemical_list: D058848:Receptors, Purinergic P2X4; D000255:Adenosine Triphosphate; D048051:p38 Mitogen-Activated Protein Kinases; D002118:Calcium