title: [Studies on intestinal absorption of alkaloids in Coptis chinensis by in situ single-pass perfused rat intestinal model].
abstract: OBJECTIVE
  To study the absorption characteristics of alkaloids in Coptis chinensis in rat intestinal.
  
  
  METHODS
  In situ single-pass perfused rat intestinal model was used and the concentrations of berberine and palmatine were determined by HPLC, the drug absorption rate constant (K(a)) and apparent absorption coefficient (P(app)) were tested to evaluate their absorption characteristics.
  
  
  RESULTS
  The absorption of berberine and palmatine in different regions of rat intestinals was significantly different. From bigness to smallness, the P(app) value of berberine at 50 mg x L(-1) were ileum, duodenum, jejunum and colon, of palmatine at 50 mg x L(-1) were ileum, colon, jejunum and duodenum. The concentration of berberine and palmatine had distinctive effect on the K(a) value of berberine and palmatine were ordered from larger to little as in Wujiwan compatibility, Coptis chinensis extractive and single compound.
  
  
  CONCLUSIONS
  The berberine and palmatine were absroped at all regions of rat intestinal. The absorption characteristics of 2 alkaloids were not passive diffusion. The other ingredients in Wujiwan compatibility and Coptis chinensis extractive could promote the absorption of alkaloids.
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.forename: Qing
authors.initials: Q
authors.lastname: Yang
authors.forename: Lijuan
authors.initials: L
authors.lastname: Zou
authors.forename: Yujie
authors.initials: Y
authors.lastname: Li
authors.forename: Yiwei
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Weng
authors.forename: Wei
authors.initials: W
authors.lastname: Men
authors.forename: Xiaoxin
authors.initials: X
authors.lastname: Zhu
mesh_terms: D000470:Alkaloids / Q000493:pharmacokinetics*; D000818:Animals; D001599:Berberine / Q000493:pharmacokinetics; D001600:Berberine Alkaloids / Q000493:pharmacokinetics; D039323:Coptis; D007408:Intestinal Absorption; D008297:Male; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000470:Alkaloids; D001600:Berberine Alkaloids; D001599:Berberine; C005413:palmatine