title: Composition, diversity, and origin of the bacterial community in grass carp intestine.
abstract: Gut microbiota has become an integral component of the host, and received increasing attention. However, for many domestic animals, information on the microbiota is insufficient and more effort should be exerted to manage the gastrointestinal bacterial community. Understanding the factors that influence the composition of microbial community in the host alimentary canal is essential to manage or improve the microbial community composition. In the present study, 16S rRNA gene sequence-based comparisons of the bacterial communities in the grass carp (Ctenopharyngodon idellus) intestinal contents and fish culture-associated environments are performed. The results show that the fish intestinal microbiota harbors many cellulose-decomposing bacteria, including sequences related to Anoxybacillus, Leuconostoc, Clostridium, Actinomyces, and Citrobacter. The most abundant bacterial operational taxonomic units (OTUs) in the grass carp intestinal content are those related to feed digestion. In addition, the potential pathogens and probiotics are important members of the intestinal microbiota. Further analyses show that grass carp intestine holds a core microbiota composed of Proteobacteria, Firmicutes, and Actinobacteria. The comparison analyses reveal that the bacterial community in the intestinal contents is most similar to those from the culture water and sediment. However, feed also plays significant influence on the composition of gut microbiota.
authors.affiliation: The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, People's Republic of China.
authors.forename: Shangong
authors.initials: S
authors.lastname: Wu
authors.forename: Guitang
authors.initials: G
authors.lastname: Wang
authors.forename: Esther R
authors.initials: ER
authors.lastname: Angert
authors.forename: Weiwei
authors.initials: W
authors.lastname: Wang
authors.forename: Wenxiang
authors.initials: W
authors.lastname: Li
authors.forename: Hong
authors.initials: H
authors.lastname: Zou
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification / Q000235:genetics*; D002347:Carps / Q000382:microbiology*; D002482:Cellulose / Q000378:metabolism; D004783:Environmental Microbiology; D015723:Gene Library; D014644:Genetic Variation; D007422:Intestines / Q000382:microbiology*; D054892:Metagenome / Q000235:genetics*; D010802:Phylogeny
chemical_list: D002482:Cellulose