title: Mannosylated liposomes for targeted gene delivery.
abstract: BACKGROUND
  Liposomes can be modified with different ligands to control their biological properties, such as longevity, targeting ability, and intracellular penetration, in a desired fashion. The aim of this study was to modify liposomes with a novel mannosylated polyethylene glycol-phosphatidylethanolamine (M-PEG-PE) ligand to achieve active targeted gene delivery.
  
  
  METHODS
  Rat Kupffer cells were isolated and used as model cells for in vitro evaluation of cytotoxicity and transfection efficiency. The modified liposomes were intravenously injected into the rats, and Kupffer cells were isolated and analyzed by flow cytometry for in vivo gene delivery and expression.
  
  
  RESULTS
  The M-PEG-PE-modified liposome-enhanced green fluorescence protein plasmid (M-PEG-PE-Lipo-pEGFP) complexes had a particle size of 237 nm and a loading efficiency of 90%. The M-PEG-PE-Lipo-pEGFP complexes displayed remarkably higher transfection efficiency than unmodified Lipo-pEGFP, both in vitro (51%-30%) and in vivo (43%-27%).
  
  
  CONCLUSIONS
  M-PEG-PE could function as an excellent active targeting ligand, and M-PEG-PE-modified liposomes could be promising active targeted drug delivery vectors.
authors.affiliation: Department of Hematology, General Hospital of Ji'nan Command, PLA, Ji'nan, People's Republic of China.
authors.forename: Fansheng
authors.initials: F
authors.lastname: Kong
authors.forename: Fang
authors.initials: F
authors.lastname: Zhou
authors.forename: Linfu
authors.initials: L
authors.lastname: Ge
authors.forename: Ximin
authors.initials: X
authors.lastname: Liu
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D016503:Drug Delivery Systems / Q000379:methods; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007728:Kupffer Cells; D008081:Liposomes / Q000008:administration & dosage* / Q000737:chemistry*; D008297:Male; D008358:Mannose / Q000008:administration & dosage* / Q000737:chemistry*; D010714:Phosphatidylethanolamines / Q000737:chemistry / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014162:Transfection / Q000379:methods*
chemical_list: D008081:Liposomes; D010714:Phosphatidylethanolamines; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; C483858:phosphatidylethanolamine; D011092:Polyethylene Glycols; D008358:Mannose