title: Glycopolymer modification on physicochemical and biological properties of poly(L-lysine) for gene delivery.
abstract: Poly(L-lysine) (PLL) has excellent plasmid DNA (pDNA) condensation capacity. However, the relatively high cytotoxicity and low transfection efficiency limit its application as gene delivery vectors. Here, well-defined glycopolymers are synthesized by reversible addition fragmentation transfer polymerization and grafted onto PLL to improve the gene delivery performance. After glycopolymer modification, PLL shows reduced cytotoxicity. By regulating the glycopolymer length and amino group substitution degree, the glycopolymer modified PLL can condense pDNA with proper strength, protect the condensed pDNA from degradation and release them in time. Transfection with NIH3T3 and HepG2 cells shows that the glycopolymer modified PLL has improved transfection efficiencies. The low cytotoxicity, effective pDNA protection and enhanced transfection efficiencies indicate that glycopolymer modification would be an effective strategy to improve the polycation properties for gene delivery.
authors.affiliation: Key Laboratory of Functional Polymer Materials (Nankai University), Ministry of Education, Institute of Polymer Chemistry, College of Chemistry, Tianjin, China.
authors.forename: Dezhong
authors.initials: D
authors.lastname: Zhou
authors.forename: Congxin
authors.initials: C
authors.lastname: Li
authors.forename: Yuling
authors.initials: Y
authors.lastname: Hu
authors.forename: Hao
authors.initials: H
authors.lastname: Zhou
authors.forename: Jiatong
authors.initials: J
authors.lastname: Chen
authors.forename: Zhengpu
authors.initials: Z
authors.lastname: Zhang
authors.forename: Tianying
authors.initials: T
authors.lastname: Guo
mesh_terms: D000818:Animals; D002236:Carbohydrate Conformation; D055598:Chemical Phenomena; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004337:Drug Carriers / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D056945:Hep G2 Cells; D006801:Humans; D051379:Mice; D008958:Models, Molecular; D041681:NIH 3T3 Cells; D009690:Nucleic Acid Conformation; D010957:Plasmids / Q000235:genetics; D011107:Polylysine / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D014162:Transfection / Q000379:methods*
chemical_list: D004337:Drug Carriers; D011134:Polysaccharides; D011107:Polylysine; D004247:DNA