title: Reduced tillering in Basmati rice T-DNA insertional mutant OsTEF1 associates with differential expression of stress related genes and transcription factors.
abstract: A T-DNA insertional mutant OsTEF1 of rice gives 60-80% reduced tillering, retarded growth of seminal roots, and sensitivity to salt stress compared to wild type Basmati 370. The insertion occurred in a gene encoding a transcription elongation factor homologous to yeast elf1, on chromosome 2 of rice. Detailed transcriptomic profiling of OsTEF1 revealed that mutation in the transcription elongation factor differentially regulates the expression of more than 100 genes with known function and finely regulates tillering process in rice by inducing the expression of cytochrome P450. Along with different transcription factors, several stress associated genes were also affected due to a single insertion. In silico analysis of the TEF1 protein showed high conservation among different organisms. This transcription elongation factor predicted to interact with other proteins that directly or indirectly positively regulate tillering in rice.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology, Roorkee 247667, India.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Paul
authors.forename: Anjali
authors.initials: A
authors.lastname: Awasthi
authors.forename: Amit Kumar
authors.initials: AK
authors.lastname: Rai
authors.forename: Santosh Kumar
authors.initials: SK
authors.lastname: Gupta
authors.forename: R
authors.initials: R
authors.lastname: Prasad
authors.forename: T R
authors.initials: TR
authors.lastname: Sharma
authors.forename: H S
authors.initials: HS
authors.lastname: Dhaliwal
mesh_terms: D020121:5' Untranslated Regions; D000595:Amino Acid Sequence; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics; D003001:Cloning, Molecular; D017124:Conserved Sequence; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics*; D015870:Gene Expression; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D018525:Germination; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D020411:Oligonucleotide Array Sequence Analysis; D012275:Oryza / Q000033:anatomy & histology / Q000235:genetics* / Q000502:physiology; D020648:Peptide Elongation Factor 1 / Q000235:genetics*; D010641:Phenotype; D018515:Plant Leaves / Q000033:anatomy & histology / Q000235:genetics / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D055049:Salt Tolerance / Q000235:genetics; D017422:Sequence Analysis, DNA; D013312:Stress, Physiological / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D020121:5' Untranslated Regions; D004269:DNA, Bacterial; D020648:Peptide Elongation Factor 1; D010940:Plant Proteins; C036483:T-DNA; D014157:Transcription Factors; D003577:Cytochrome P-450 Enzyme System