title: Lysinibacillus mangiferahumi sp. nov., a new bacterium producing nematicidal volatiles.
abstract: A Gram-positive bacterium, designated M-GX18(T), was isolated from the rhizosphere soil of mango (Guangxi Province, China). The isolate produced nematicidal volatile compounds with activities against the root-knot nematode Meloidogyne incognita. The organism was motile, catalase- and oxidase-positive, spore-forming and rod shaped. The predominant menaquinone was MK-7 and the major cellular fatty acid profiles were iso-C(15:0), anteiso-C(15:0) and iso-C(16:0). The DNA G+C content was 38.9%. A phylogenetic analysis based on 16S rRNA gene sequences showed that this organism represented a new species of the genus Lysinibacillus. Strain M-GX18(T) exhibited high 16S rRNA gene sequence similarity with its closest neighbors Lysinibacillus sphaericus (98.5%), Lysinibacillus fusiformis (98.1%) and Lysinibacillus xylanilyticus (98.6%). The physiological, biochemical and chemotaxonomic data, including DNA-DNA hybridization relatedness data, indicate that strain M-GX18(T) can be distinguished from all the related species of the genus Lysinibacillus. Therefore, on the basis of the polyphasic taxonomic data presented, a new species of the genus Lysinibacillus, Lysinibacillus mangiferahumi, with the type strain M-GX18(T) (=DSM 24076(T) = CCTCC AB 2010389(T)) is proposed.
authors.affiliation: Laboratory for Conservation and Utilization of Bio-Resources & Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming, 650091, People's Republic of China.
authors.forename: Ling-Ling
authors.initials: LL
authors.lastname: Yang
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.forename: Li
authors.initials: L
authors.lastname: Ma
authors.forename: Ming-He
authors.initials: MH
authors.lastname: Mo
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
authors.forename: Fa-Xiang
authors.initials: FX
authors.lastname: Yang
mesh_terms: D000818:Animals; D000969:Antinematodal Agents / Q000378:metabolism*; D001406:Bacillaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002374:Catalase / Q000378:metabolism; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008124:Locomotion; D031022:Mangifera / Q000382:microbiology*; D008969:Molecular Sequence Data; D009822:Oils, Volatile / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013171:Spores, Bacterial / Q000166:cytology; D014431:Tylenchoidea / Q000187:drug effects*; D024482:Vitamin K 2 / Q000032:analysis
chemical_list: D000969:Antinematodal Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D009822:Oils, Volatile; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D010088:Oxidoreductases; D002374:Catalase