title: Changes in ribosomal protein S3 immunoreactivity and its protein levels in the gerbil hippocampus following subacute and chronic restraint stress.
abstract: Ribosomal protein S3 (rpS3), a multi-functional protein, has been known to participate in DNA repair mechanism. In this study, we investigated changes in rpS3 immunoreactivity and its protein levels in the sub-regions of the gerbil hippocampus following subacute and chronic restraint stress. Serum corticosterone levels were increased in both the subacute and chronic-stress-groups compared to the control-group: the level in the subacute-stress-group was much higher than that in the chronic-stress-group. We could not find any neuronal damage in all the sub-regions of the hippocampus after both the subacute and chronic restraint stress. In the subacute-stress-group, rps3 immunoreactivity was not different compared to the control-group. However, rps3 immunoreactivity in the chronic-stress-group was decreased compared to the subacute-stress-group: especially, the immunoreactivity was markedly decreased in the pyramidal cells of the hippocampus proper (CA1-CA3 region) and granule cells of the dentate gyrus. In addition, western blot analysis also showed that rpS3 protein levels in the chronic-stress-group were significantly decreased compared to those in the subacute-stress-group. These findings indicate that chronic stress, not subacute stress, can decrease rpS3 immunoreactivity.
authors.affiliation: Department of Neurobiology, Institute of Medical Sciences, School of Medicine, Kangwon National University, Chuncheon, 200-701, South Korea.
authors.forename: Joon Ha
authors.initials: JH
authors.lastname: Park
authors.forename: Choong Hyun
authors.initials: CH
authors.lastname: Lee
authors.forename: Bing Chun
authors.initials: BC
authors.lastname: Yan
authors.forename: Ji Hyeon
authors.initials: JH
authors.lastname: Ahn
authors.forename: Young Joo
authors.initials: YJ
authors.lastname: Lee
authors.forename: Chan Woo
authors.initials: CW
authors.lastname: Park
authors.forename: Jun Hwi
authors.initials: JH
authors.lastname: Cho
authors.forename: Soo Young
authors.initials: SY
authors.lastname: Choi
authors.forename: Yun Lyul
authors.initials: YL
authors.lastname: Lee
authors.forename: Moo-Ho
authors.initials: MH
authors.lastname: Won
authors.forename: Hui Young
authors.initials: HY
authors.lastname: Lee
mesh_terms: D000818:Animals; D003345:Corticosterone / Q000378:metabolism; D005849:Gerbillinae; D006624:Hippocampus / Q000378:metabolism*; D007103:Immobilization; D012269:Ribosomal Proteins / Q000378:metabolism*; D013312:Stress, Physiological
chemical_list: D012269:Ribosomal Proteins; C044152:ribosomal protein S3; D003345:Corticosterone