title: Improvement of D-lactate productivity in recombinant Escherichia coli by coupling production with growth.
abstract: Coupling lactate fermentation with cell growth was investigated in shake-flask and bioreactor cultivation systems by increasing aeration to improve lactate productivity in Escherichia coli CICIM B0013-070 (ackA pta pps pflB dld poxB adhE frdA). In shake-flasks, cells reached 1 g dry wt/l then, cultivated at 100 rpm and 42°C, achieved a twofold higher productivity of lactic acid compared to aerobic and O(2)-limited two-phase fermentation. The cells in the bioreactor yielded an overall volumetric productivity of 5.5 g/l h and a yield of 86 g lactic acid/100 g glucose which were 66% higher and the same level compared to that of the aerobic and O(2)-limited two-phase fermentation, respectively, using scaled-up conditions optimized from shake-flask experiments. These results have revealed an approach for improving production of fermentative products in E. coli.
authors.affiliation: Center for Bioresource and Bioenergy, School of Biotechnology, Jiangnan University, Wuxi, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
authors.forename: Kang-Ming
authors.initials: KM
authors.lastname: Tian
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Niu
authors.forename: Wei
authors.initials: W
authors.lastname: Shen
authors.forename: Gui-Yang
authors.initials: GY
authors.lastname: Shi
authors.forename: Suren
authors.initials: S
authors.lastname: Singh
authors.forename: Zheng-Xiang
authors.initials: ZX
authors.lastname: Wang
mesh_terms: D000332:Aerobiosis; D018533:Biomass; D019149:Bioreactors; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D005285:Fermentation; D019344:Lactic Acid / Q000378:metabolism*; D060847:Metabolic Engineering
chemical_list: D019344:Lactic Acid