title: Plasma persistence of 2-aminothiazoline-4-carboxylic acid in rat system determined by liquid chromatography tandem mass spectrometry.
abstract: 2-Aminothiazoline-4-carboxylic acid (ATCA) was intravenously injected to rats in order to investigate its plasma distribution. ATCA was extracted from plasma samples by solid phase extraction (SPE) and molecularly imprinted polymer stir bar sorption extraction (MIP-SBSE). Detection and quantification of ATCA were achieved by using liquid chromatography-tandem mass spectrometry (LC-MS/MS). It was found that the intravenously injected ATCA concentration quickly decreased to half within 2.5h in the rat system. However, after 2.5 h, the concentration of ATCA in plasma stayed constant at least 5 folds above the endogenous ATCA level for more then 48 h. This finding can be used for evaluating ATCA's diagnostic and forensic value as a biomarker for cyanide exposure.
authors.affiliation: Department of Chemistry, Sam Houston State University, Huntsville, TX 77341, USA. ixp004@shsu.edu
authors.forename: Ilona
authors.initials: I
authors.lastname: Petrikovics
authors.forename: Jorn C C
authors.initials: JC
authors.lastname: Yu
authors.forename: David E
authors.initials: DE
authors.lastname: Thompson
authors.forename: Prashanth
authors.initials: P
authors.lastname: Jayanna
authors.forename: Brian A
authors.initials: BA
authors.lastname: Logue
authors.forename: Jessica
authors.initials: J
authors.lastname: Nasr
authors.forename: Raj K
authors.initials: RK
authors.lastname: Bhandari
authors.forename: Steven I
authors.initials: SI
authors.lastname: Baskin
authors.forename: Gary
authors.initials: G
authors.lastname: Rockwood
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D008297:Male; D051381:Rats; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013844:Thiazoles / Q000097:blood*
chemical_list: D013844:Thiazoles; C015674:2-amino-delta(2)-thiazoline-4-carboxylic acid