title: A unique series of reversibly switchable fluorescent proteins with beneficial properties for various applications.
abstract: Reversibly switchable fluorescent proteins (RSFPs) have attracted widespread interest for emerging techniques including repeated tracking of protein behavior and superresolution microscopy. Among the limited number of RSFPs available, only Dronpa is widely employed for most cell biology applications due to its monomeric and other favorable photochemical properties. Here we developed a series of monomeric green RSFPs with beneficial optical characteristics such as high photon output per switch, high photostability, a broad range of switching rate, and pH-dependence, which make them potentially useful for various applications. One member of this series, mGeos-M, exhibits the highest photon budget and localization precision potential among all green RSFPs. We propose mGeos-M as a candidate to replace Dronpa for applications such as dynamic tracking, dual-color superresolution imaging, and optical lock-in detection.
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, People's Republic of China.
authors.forename: Hao
authors.initials: H
authors.lastname: Chang
authors.forename: Mingshu
authors.initials: M
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Ji
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Chen
authors.forename: Yongdeng
authors.initials: Y
authors.lastname: Zhang
authors.forename: Bei
authors.initials: B
authors.lastname: Liu
authors.forename: Jingze
authors.initials: J
authors.lastname: Lu
authors.forename: Junlong
authors.initials: J
authors.lastname: Zhang
authors.forename: Pingyong
authors.initials: P
authors.lastname: Xu
authors.forename: Tao
authors.initials: T
authors.lastname: Xu
mesh_terms: D002460:Cell Line; D049452:Green Fluorescent Proteins / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008853:Microscopy / Q000379:methods; D018613:Microscopy, Confocal / Q000379:methods; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D009154:Mutation; D010777:Photochemistry / Q000379:methods; D017785:Photons; D013053:Spectrophotometry / Q000379:methods
chemical_list: D049452:Green Fluorescent Proteins