title: Culture and proliferation of highly purified adult Schwann cells from rat, dog, and man.
abstract: This chapter presents fast and easy protocols to obtain highly purified cultures of proliferating adult rat, canine, and human Schwann cells. Cell preparation from predegenerated adult sciatic nerves combined with the use of melanocyte growth medium supplemented with forskolin, fibroblast growth factor-2, pituitary extract, and heregulin as selective, serum-free culture medium and two methods for a consecutive cell-enrichment step are described. Our protocols result in approximately 90% pure Schwann cell cultures (or higher). The average time to obtain highly purified in vitro cultures of adult Schwann cells is 21 days.
authors.affiliation: Hannover Medical School, Institute of Neuroanatomy, Hannover, Germany. Haastert.kirsten@mh-hannover.de
authors.forename: Kirsten
authors.initials: K
authors.lastname: Haastert-Talini
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods*; D005576:Colforsin; D003470:Culture Media / Q000737:chemistry; D004285:Dogs; D016222:Fibroblast Growth Factor 2; D006801:Humans; D020890:Neuregulin-1; D051381:Rats; D012583:Schwann Cells / Q000166:cytology* / Q000502:physiology*
chemical_list: D003470:Culture Media; D020890:Neuregulin-1; D016222:Fibroblast Growth Factor 2; D005576:Colforsin