title: Extraction of high-quality genomic DNA from Ectocarpus.
abstract: For some applications, such as genome sequencing and high-throughput genotyping with multiple markers, it is necessary to use high-quality genomic DNA. This article describes how to obtain several micrograms of high-quality, cesium chloride-purified DNA from 1 g of Ectocarpus filaments. We also recommend using DNA of this quality for quantitative RT-PCR control reactions. However, simpler, more rapid, kit-based methods are preferable for experiments that involve the treatment of large numbers of individuals, such as genotyping large populations with a small number of markers or PCR screening of large populations.
authors.affiliation: UPMC Université Paris 06, The Marine Plants and Biomolecules Laboratory, UMR 7139, Station Biologique de Roscoff, 29682 Roscoff Cedex, France.
authors.forename: Susana M
authors.initials: SM
authors.lastname: Coelho
authors.forename: Delphine
authors.initials: D
authors.lastname: Scornet
authors.forename: Sylvie
authors.initials: S
authors.lastname: Rousvoal
authors.forename: Nick
authors.initials: N
authors.lastname: Peters
authors.forename: Laurence
authors.initials: L
authors.lastname: Dartevelle
authors.forename: Akira F
authors.initials: AF
authors.lastname: Peters
authors.forename: J Mark
authors.initials: JM
authors.lastname: Cock
mesh_terms: D004247:DNA / Q000302:isolation & purification*; D008967:Molecular Biology / Q000379:methods*; D058889:Molecular Typing; D000459:Phaeophyceae / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D004247:DNA