title: [Identification of Schisandra sphenanthera and S. chinensis by random amplified polymorphic DNA sequence characterized applied region].
abstract: OBJECTIVE
  To establish a new method for the identification of Schisandra sphenanthera and S. chinensis.
  
  
  METHODS
  Random amplified polymorphic DNA-Sequence characterized applied region (RAPD-SCAR) method was applied to screen primers.
  
  
  RESULTS
  Screening from 100 primers, only 2 random primers, which can be used to identify S. sphenanthera and S. chinensis accurately with a good reproducibility. It worked to fit them into sequence characterized applied region.
  
  
  CONCLUSIONS
  RAPD-SCAR can be used to identify S. sphenanthera and S. chinensis accurately.
authors.affiliation: Bioscience and Technology Institute of Shenyang Agriculture University, Shenyang 110161, China. chenlijing1997@126.com
authors.forename: Lijing
authors.initials: L
authors.lastname: Chen
authors.forename: Xin
authors.initials: X
authors.lastname: Qi
authors.forename: Yukun
authors.initials: Y
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Zhifu
authors.initials: Z
authors.lastname: Guo
authors.forename: Jingwei
authors.initials: J
authors.lastname: Lin
authors.forename: Yuning
authors.initials: Y
authors.lastname: Song
authors.forename: Ming
authors.initials: M
authors.lastname: Zhong
mesh_terms: D001483:Base Sequence; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D019105:Random Amplified Polymorphic DNA Technique; D032141:Schisandra / Q000235:genetics*; D017422:Sequence Analysis, DNA