title: CR3-dependent negative regulation of human eosinophils by Mycobacterium bovis BCG lipoarabinomannan.
abstract: Eosinophils have recently been shown to participate in innate immune responses against mycobacteria. We have investigated whether Mycobacterium bovis BCG regulate the human eosinophil immune response. A negative correlation between mycobacteria internalization and eosinophil activation was observed. In addition, mannose-capped lipoarabinomannan from M. bovis BCG (ManLAM) failed to induce a significant release of eosinophil peroxidase and TNF-α. Noteworthy, ManLAM exhibited a potent inhibitory effect on eosinophil peroxidase release by TLR2-activated eosinophils involving the complement receptor-3 molecule and the phosphatidylinositol-3 kinase pathway. ManLAM, generally present in pathogenic mycobacteria, plays an important role in modulating eosinophil-dependent immune response.
authors.affiliation: Inserm, U547, Lille, France; Université Lille 2, Lille, France.
authors.forename: Virginie
authors.initials: V
authors.lastname: Driss
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Hermann
authors.forename: Fanny
authors.initials: F
authors.lastname: Legrand
authors.forename: Sylvie
authors.initials: S
authors.lastname: Loiseau
authors.forename: Marie
authors.initials: M
authors.lastname: Delbeke
authors.forename: Laurent
authors.initials: L
authors.lastname: Kremer
authors.forename: Yann
authors.initials: Y
authors.lastname: Guerardel
authors.forename: David
authors.initials: D
authors.lastname: Dombrowicz
authors.forename: Monique
authors.initials: M
authors.lastname: Capron
mesh_terms: D004804:Eosinophils / Q000276:immunology* / Q000378:metabolism*; D004921:Erythropoietin / Q000378:metabolism; D006801:Humans; D008070:Lipopolysaccharides / Q000276:immunology*; D016177:Macrophage-1 Antigen / Q000378:metabolism*; D009163:Mycobacterium bovis / Q000276:immunology*; D010587:Phagocytosis / Q000276:immunology; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D051195:Toll-Like Receptor 2 / Q000378:metabolism
chemical_list: D008070:Lipopolysaccharides; D016177:Macrophage-1 Antigen; D051195:Toll-Like Receptor 2; C050016:lipoarabinomannan; D004921:Erythropoietin; D019869:Phosphatidylinositol 3-Kinases