title: Development of a reverse transcription loop-mediated isothermal amplification assay for H7N7 equine influenza virus.
abstract: We have developed a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for detecting H7N7 equine influenza virus (EIV). The detection limit of the RT-LAMP assay was a virus dilution of 10(-4), which was 10 times more sensitive than that of Espline Influenza A&B-N and the same as that of reverse transcription polymerase chain reaction. The RT-LAMP assay specifically amplified H7N7 EIV strains but did not amplify several pathogens related to equine respiratory disease including H3N8 EIV strains. Because it provides ease of manipulation, the RT-LAMP assay is suitable for large-scale surveillance for H7N7 EIV. In addition, the combination of the H3N8 RT-LAMP assay, which was developed previously, with the H7N7 RT-LAMP assay should be useful to discriminate between H3N8 and H7N7 EIVs in clinical laboratories.
authors.affiliation: Epizootic Research Center, Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan. nemoto_manabu@epizoo.equinst.go.jp
authors.forename: Manabu
authors.initials: M
authors.lastname: Nemoto
authors.forename: Takashi
authors.initials: T
authors.lastname: Yamanaka
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Bannai
authors.forename: Koji
authors.initials: K
authors.lastname: Tsujimura
authors.forename: Takashi
authors.initials: T
authors.lastname: Kondo
authors.forename: Tomio
authors.initials: T
authors.lastname: Matsumura
mesh_terms: D000818:Animals; D062665:Epidemiological Monitoring / Q000662:veterinary*; D006734:Horse Diseases / Q000175:diagnosis* / Q000821:virology*; D006736:Horses; D053126:Influenza A Virus, H7N7 Subtype / Q000235:genetics*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods / Q000662:veterinary*; D009976:Orthomyxoviridae Infections / Q000662:veterinary* / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D048348:Reverse Transcription / Q000502:physiology*