title: Single strand DNA catenane synthesis using the formation of G-quadruplex structure.
abstract: DNA is a good material for constructing nanostructures such as DNA origami. One of the challenges in this field is constructing a topologically complex structure. Here, we synthesized a DNA catenane through the formation of a G-quadruplex structure. The formation of the DNA catenane was investigated by gel electrophoresis. Interestingly, the synthesized DNA catenane was destroyed by heat treatment. Because conventional methods to construct DNA catenane include enzymatic ligation or chemical reactions, DNA is cyclized by covalent bond connection and never destroyed by heat treatment. To our knowledge, this is the first report of the synthesis of DNA catenane without using covalent bonds. Our novel way of synthesizing DNA catenane may be of use in easily recoverable DNA topological labeling.
authors.affiliation: Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan.
authors.forename: Yuta
authors.initials: Y
authors.lastname: Sannohe
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sugiyama
mesh_terms: D000873:Anthracenes / Q000138:chemical synthesis / Q000737:chemistry*; D001483:Base Sequence; D060326:Chemistry Techniques, Synthetic / Q000379:methods; D004277:DNA, Single-Stranded / Q000138:chemical synthesis / Q000737:chemistry*; D054856:G-Quadruplexes; D006358:Hot Temperature; D049329:Nanostructures / Q000737:chemistry*
chemical_list: D000873:Anthracenes; D004277:DNA, Single-Stranded; C467767:catenane