title: High-throughput capture of nucleotide sequence polymorphisms in three Brassica species (Brassicaceae) using DNA microarrays.
abstract: OBJECTIVE
  To capture molecular markers that are applicable to environmental risk assessment of genetically modified oilseed rape, and to streamline their development, we screened variations in nucleotide sequences of three Brassica species by DNA microarray analysis.
  
  
  RESULTS
  Using the Affymetrix GeneChip Arabidopsis ATH1 Genome Array, we monitored gene expression at 22810 loci among the Brassica species and picked out 192 putative polymorphic loci. We sequenced 25 of these and successfully aligned them among all three species. All 25 loci possessed some interspecific and at times intraspecific nucleotide variation.
  
  
  CONCLUSIONS
  DNA microarray analysis effectively detected a large number of nucleotide sequence variations among closely related Brassica species. The polymorphic regions will allow the subsequent development of functional gene markers.
authors.affiliation: Environmental Biology Division, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki 305-8506, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Nishizawa
authors.forename: Masanori
authors.initials: M
authors.lastname: Tamaoki
authors.forename: Yukio
authors.initials: Y
authors.lastname: Kaneko
authors.forename: Mitsuko
authors.initials: M
authors.lastname: Aono
authors.forename: Akihiro
authors.initials: A
authors.lastname: Kubo
authors.forename: Hikaru
authors.initials: H
authors.lastname: Saji
authors.forename: Nobuyoshi
authors.initials: N
authors.lastname: Nakajima
mesh_terms: D001937:Brassica / Q000145:classification / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D020869:Gene Expression Profiling; D020411:Oligonucleotide Array Sequence Analysis; D011110:Polymorphism, Genetic; D013045:Species Specificity
chemical_list: D018744:DNA, Plant