title: Delineation of commonly deleted chromosomal regions in meningiomas by high-density single nucleotide polymorphism genotyping arrays.
abstract: Despite recent advances in the identification of the cytogenetic profiles of meningiomas, a significant group of tumors still show normal karyotypes or few chromosomal changes. The authors analyzed the cytogenetic profile of 50 meningiomas using fluorescence in situ hybridization and high-density (500 K) single nucleotide polymorphism (SNP) arrays. Our results confirm that del(22q) (52%) and del(1p) (16%) (common deleted regions: 22q11.21-22q13.3. and 1p31.2-p36.33) are the most frequent alterations. Additionally, recurrent monosomy 14 (8%), del(6q) (10%), del(7p) (10%), and del(19q) (4%) were observed, while copy number patterns consistent with recurrent chromosomal gains, gene amplification, and copy number neutral loss of heterozygosity (cnLOH) were either absent or rare. Based on their overall SNP profiles, meningiomas could be classified into: (i) diploid cases, (ii) meningiomas with a single chromosomal change [e.g., monosomy 22/del(22q)] and (iii) tumors with ≥2 altered chromosomes. In summary, our results confirm and extend on previous observations showing that the most recurrent chromosomal abnormalities in meningiomas correspond to chromosome losses localized in chromosomes 1, 22 and less frequently in chromosomes 6, 7, 14, and 19, while chromosomal gains and cnLOH are restricted to a small proportion of cases. Finally, a set of cancer-associated candidate genes associated with the TP53, MYC, CASP3, HDAC1, and TERT signaling pathways was identified, in cases with coexisting monosomy 14 and del(1p).
authors.affiliation: IECSCYL, Soria, Spain. mdtabernero@iecscyl.com
authors.forename: Maria Dolores
authors.initials: MD
authors.lastname: Tabernero
authors.forename: Angel
authors.initials: A
authors.lastname: Maíllo
authors.forename: Ana Belen
authors.initials: AB
authors.lastname: Nieto
authors.forename: Cristina
authors.initials: C
authors.lastname: Diez-Tascón
authors.forename: Mónica
authors.initials: M
authors.lastname: Lara
authors.forename: Pablo
authors.initials: P
authors.lastname: Sousa
authors.forename: Alvaro
authors.initials: A
authors.lastname: Otero
authors.forename: Abel
authors.initials: A
authors.lastname: Castrillo
authors.forename: Maria del Carmen
authors.initials: Mdel C
authors.lastname: Patino-Alonso
authors.forename: Ana
authors.initials: A
authors.lastname: Espinosa
authors.forename: Carlos
authors.initials: C
authors.lastname: Mackintosh
authors.forename: Enrique
authors.initials: E
authors.lastname: de Alava
authors.forename: Alberto
authors.initials: A
authors.lastname: Orfao
mesh_terms: D000328:Adult; D000368:Aged; D002872:Chromosome Deletion; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008577:Meningeal Neoplasms / Q000235:genetics*; D008579:Meningioma / Q000235:genetics*; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D020641:Polymorphism, Single Nucleotide; D015398:Signal Transduction