title: Extracellular matrix secreted by reactive stroma is a main inducer of pro-tumorigenic features on LNCaP prostate cancer cells.
abstract: Tumor microenvironment modifications are related to the generation of reactive stroma and to critical events in cancer progression, such as proliferation, migration and apoptosis. In order to clarify these cellular interactions mediated by reactive stroma, we investigated the effects of cell-cell contacts, and the influence of soluble factors and extracellular matrix (ECM) secreted by Benign Prostate Hyperplasia (BPH) reactive stroma over LNCaP prostate tumor cells. Using in vitro functional assays, we demonstrated that ECM strongly stimulated LNCaP cell proliferation and migration, while inhibiting apoptosis, and inducing a deregulated expression pattern of several genes related to prostate cancer (PCa) progression. Conversely, reactive stromal cells per se and their secreted conditioned medium partially modulated these pro-tumorigenic events. These data indicate that secreted ECM in reactive stroma microenvironment contains key molecules that positively modulate important cancer hallmarks.
authors.affiliation: Programa de Pesquisa em Biologia Celular e do Desenvolvimento, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Antonio
authors.initials: A
authors.lastname: Palumbo
authors.forename: Luciana Bueno
authors.initials: LB
authors.lastname: Ferreira
authors.forename: Pedro A V
authors.initials: PA
authors.lastname: Reis de Souza
authors.forename: Felipe Leite de
authors.initials: FL
authors.lastname: Oliveira
authors.forename: Bruno
authors.initials: B
authors.lastname: Pontes
authors.forename: Nathan B
authors.initials: NB
authors.lastname: Viana
authors.forename: Daniel Escorsim
authors.initials: DE
authors.lastname: Machado
authors.forename: Celia Yelimar
authors.initials: CY
authors.lastname: Palmero
authors.forename: Leandro M
authors.initials: LM
authors.lastname: Alves
authors.forename: Etel R P
authors.initials: ER
authors.lastname: Gimba
authors.forename: Luiz E
authors.initials: LE
authors.lastname: Nasciutti
mesh_terms: D000818:Animals; D002450:Cell Communication; D045744:Cell Line, Tumor; D002470:Cell Survival; D018450:Disease Progression; D005109:Extracellular Matrix / Q000473:pathology*; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D011470:Prostatic Hyperplasia / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000378:metabolism / Q000473:pathology*; D017154:Stromal Cells / Q000378:metabolism; D059016:Tumor Microenvironment / Q000502:physiology*