title: Staining of Bartonella henselae with carboxyfluorescein diacetate succinimidyl ester for tracking infection in erythrocytes and epithelial cells.
abstract: Bartonella infection (Bartonella henselae in particular) is responsible for a widening spectrum of human diseases. The persistent colonization of erythrocytes is a feature of Bartonella infection. Endothelial and epithelial cells are also widely used to study the pathogenesis of bartonellosis in vitro. Exploring a convenient method for visualizing the bacillus without affecting infectivity would be very interesting. Carboxyfluorescein diacetate succinimidyl ester (CFSE) has been previously used for staining several bacterial species to study their adhesion to host cells. The present study demonstrated the efficiency and safety of using CFSE in staining B. henselae. The staining of bacillus-invaded erythrocytes and epithelial cells in vitro successfully allowed for flow cytometry and confocol microscopy analyses. Parallel tests using untreated bacteria confirmed that CFSE staining did not result in side effects on the infectivity of B. henselae. Labeling Bartonella with CFSE is a valuable method for studying the bacteria-host interaction.
authors.affiliation: Shanghai Jiaotong University, Shanghai, PR China.
authors.forename: Congli
authors.initials: C
authors.lastname: Yuan
authors.forename: Caixia
authors.initials: C
authors.lastname: Zhu
authors.forename: Yajie
authors.initials: Y
authors.lastname: Bai
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Yang
authors.forename: Xiuguo
authors.initials: X
authors.lastname: Hua
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D001474:Bartonella Infections / Q000175:diagnosis* / Q000382:microbiology; D018416:Bartonella henselae / Q000166:cytology*; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000382:microbiology*; D004912:Erythrocytes / Q000382:microbiology*; D005434:Flow Cytometry / Q000379:methods; D005452:Fluoresceins / Q000378:metabolism*; D006805:Human Experimentation; D006801:Humans; D018613:Microscopy, Confocal / Q000379:methods; D013194:Staining and Labeling / Q000379:methods*; D013388:Succinimides / Q000378:metabolism*
chemical_list: C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester; D005452:Fluoresceins; D013388:Succinimides