title: [Selection and anti-cancer effects of siRNAs targeting HMGA2 gene].
abstract: High mobility group A2 protein (HMGA2), an architectural factor, is highly expressed in various cancer types including lung cancers. It is a candidate target for cancer therapy. RNAi is an effective gene silencing method with low cost and less time-consuming. It is possible to exploit this technology in therapy. Here, 5 siRNAs targeting Hmga2 gene (HMGA2 siRNA1-5) were designed and synthesized. MTT assay, colony formation assay, transwell assay and flow cytometry were used to evaluate the effects of these siRNAs on lung cancer cell lines (NCI-H446 and A549). Results from cell proliferation, clone formation, migration and apoptosis showed that HMGA2 siRNA1, 3, 5 could affect these aspects for both lung cancer cell lines. Among the five siRNAs, HMGA2 siRNA5 showed the greatest inhibition effects. The inhibition effects of HMGA2 siRNA5 are sequence specific and are not due to the induction of interferon response. Taken together, siRNAs targeting Hmga2 gene are potential candidates for lung cancer gene therapy.
authors.affiliation: Institute of Molecular Medicine, Huaqiao University, Quanzhou 362021, China.
authors.forename: Qi-Zhao
authors.initials: QZ
authors.lastname: Wang
authors.forename: Yu-Hua
authors.initials: YH
authors.lastname: Gong
authors.forename: Ying-Hui
authors.initials: YH
authors.lastname: Lü
authors.forename: Ling-Na
authors.initials: LN
authors.lastname: Fei
authors.forename: Hui-Jie
authors.initials: HJ
authors.lastname: Liu
authors.forename: Yong
authors.initials: Y
authors.lastname: Diao
authors.forename: Rui-An
authors.initials: RA
authors.lastname: Xu
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D003114:Colony-Forming Units Assay; D020868:Gene Silencing; D015316:Genetic Therapy; D025743:HMGA2 Protein / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007372:Interferons / Q000378:metabolism; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D017354:Point Mutation; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics*; D014162:Transfection
chemical_list: D025743:HMGA2 Protein; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D007372:Interferons