title: Magnetic nanoparticles: an improved method for mitochondrial isolation.
abstract: The ultrastructure, function, and physical and chemical properties of mitochondria             have become important issues in scientific research. Current mitochondrial isolation             methods mainly rely on the physical and chemical properties of mitochondria. Our             team presents a fast and reliable new isolation method based on magnetic nanoparticles             binding with monoamine oxidase-A (MAO-A) expressed in the mitochondria. MAO-A             is expressed in the outer membrane of human mitochondria and is localized on the             cytoplasmic side of the membrane, which makes it possible to isolate the mitochondria             effectively, using a magnetic field. As shown in the present study, in comparison             with differential centrifugation and density gradient centrifugation, the yield             of mitochondria isolated by magnetic nanoparticle binding is higher, with greater             mitochondrial purity and activity. The entire process, from cell harvesting to             final isolation of the mitochondria, takes approximately 1 h. Magnetic nanoparticles             provide a simple, practical approach for mitochondrial isolation.
authors.affiliation: Department of General Surgery, The Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116027, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Tang
authors.forename: Liang
authors.initials: L
authors.lastname: Zhao
authors.forename: Rui
authors.initials: R
authors.lastname: Liang
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Liming
authors.initials: L
authors.lastname: Wang
mesh_terms: D002458:Cell Fractionation; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008928:Mitochondria / Q000737:chemistry*; D051336:Mitochondrial Membranes / Q000737:chemistry*; D008995:Monoamine Oxidase / Q000737:chemistry*
chemical_list: D058185:Magnetite Nanoparticles; D008995:Monoamine Oxidase