title: Identification of reproduction-related proteins and characterization of proteasome alpha 3 and proteasome beta 6 cDNAs in testes of the giant tiger shrimp Penaeus monodon.
abstract: Cellular proteomic analysis was carried out to identify reproduction-related proteins in testes of wild and domesticated broodstock of Penaeus monodon. In total, 642 protein spots were characterized and 287 spots (44.70%) significantly matched protein sequences in the databases (P<0.05). To examine a role of the proteasome system in testicular development of P. monodon, the expression profiles of proteasome alpha 3 subunit (PmPsma3) and proteasome beta 6 (PmPsmb6) mRNA in different groups of domesticated shrimp and in wild broodstock were examined. The expression levels of these transcripts in testes of 18-month-old domesticated shrimp were significantly lower than those of wild broodstock (P<0.05). Interestingly, the expression levels of testicular PmPsma3 and PmPsmb6 in 18-month-old shrimp were significantly increased at 24 h following serotonin injection (50 μg/g body weight). Results suggested that reduced degrees of maturation in captive P. monodon males may be partially resolved by exogenous 5-HT administration.
authors.affiliation: National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Klong 1, Klong Luang, Pathumthani 12120, Thailand. sirawut@biotec.or.th
authors.forename: Sirawut
authors.initials: S
authors.lastname: Klinbunga
authors.forename: Sasithorn
authors.initials: S
authors.lastname: Petkorn
authors.forename: Suthathip
authors.initials: S
authors.lastname: Kittisenachai
authors.forename: Narumon
authors.initials: N
authors.lastname: Phaonakrop
authors.forename: Sittiruk
authors.initials: S
authors.lastname: Roytrakul
authors.forename: Bavornlak
authors.initials: B
authors.lastname: Khamnamtong
authors.forename: Piamsak
authors.initials: P
authors.lastname: Menasveta
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000829:Animals, Domestic / Q000235:genetics*; D000835:Animals, Wild / Q000235:genetics*; D018076:DNA, Complementary / Q000096:biosynthesis; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D008297:Male; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000187:drug effects / Q000235:genetics*; D046988:Proteasome Endopeptidase Complex / Q000235:genetics* / Q000378:metabolism; D040901:Proteomics; D012333:RNA, Messenger / Q000096:biosynthesis; D012098:Reproduction / Q000235:genetics*; D012701:Serotonin / Q000008:administration & dosage; D013737:Testis / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D012701:Serotonin; D046988:Proteasome Endopeptidase Complex