title: Isolation of natural products by preparative high performance liquid chromatography (prep-HPLC).
abstract: Preparative HPLC (prep-HPLC) has emerged as a mainstay of natural products isolation and purification. The various modes available to date, e.g. normal phase, reversed-phase, size exclusion, and ion exchange, can be used to purify most classes of natural products. This chapter presents an overview of the different modes along with a practical guide as to how to purify a natural product using the most robust and widely used of the modes, namely reversed-phase prep-HPLC. Instrumentation set-up and detection methods, sample preparation, method development, and sample work up are also discussed.
authors.affiliation: Technology Strategy Board, Swindon, UK.
authors.forename: Zahid
authors.initials: Z
authors.lastname: Latif
authors.forename: Satyajit D
authors.initials: SD
authors.lastname: Sarker
mesh_terms: D000818:Animals; D001688:Biological Products / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D056148:Chromatography, Reverse-Phase / Q000379:methods; D028481:Euphorbia / Q000737:chemistry; D005419:Flavonoids / Q000302:isolation & purification; D006027:Glycosides / Q000302:isolation & purification; D006035:Glycyrrhiza / Q000737:chemistry; D006801:Humans; D010936:Plant Extracts / Q000302:isolation & purification*; D031965:Ribes / Q000737:chemistry; D012997:Solvents / Q000737:chemistry
chemical_list: D001688:Biological Products; D005419:Flavonoids; D006027:Glycosides; D010936:Plant Extracts; D012997:Solvents