title: Characterization of a commercial cellulase for hydrolysis of agroindustrial substrates.
abstract: This work is focused on the characterization of a commercial cellulase in terms of optimum pH and temperature, stability to pH and temperature and affinity of this enzyme to several substrates, determining the Michaelis-Menten parameters. Maximum activity of cellulase was obtained for the temperature range from 40 to 50 °C and pH from 5.2 to 5.5. Enzyme activity decreased only 15% after 150 h of reaction at temperatures between 30 and 50 °C. No loss of activity was observed at pH 5.0 and 5.5. The cellulase showed satisfactory results in the hydrolysis of agroindustrial substrates, since similar activity was verified on filter paper and other agroindustrial substrates.
authors.affiliation: Department of Food Engineering, URI, Campus de Erechim, Erechim, Brazil.
authors.forename: Guilherme
authors.initials: G
authors.lastname: Balsan
authors.forename: Viviane
authors.initials: V
authors.lastname: Astolfi
authors.forename: Tássio
authors.initials: T
authors.lastname: Benazzi
authors.forename: M Angela A
authors.initials: MA
authors.lastname: Meireles
authors.forename: Francisco
authors.initials: F
authors.lastname: Maugeri
authors.forename: Marco
authors.initials: M
authors.lastname: Di Luccio
authors.forename: Valéria
authors.initials: V
authors.lastname: Dal Prá
authors.forename: Altemir J
authors.initials: AJ
authors.lastname: Mossi
authors.forename: Helen
authors.initials: H
authors.lastname: Treichel
authors.forename: Marcio A
authors.initials: MA
authors.lastname: Mazutti
mesh_terms: D000383:Agriculture; D002480:Cellulase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D013696:Temperature
chemical_list: D002480:Cellulase