title: Tertiary DNA structure in the single-stranded hTERT promoter fragment unfolds and refolds by parallel pathways via cooperative or sequential events.
abstract: The discovery of G-quadruplexes and other DNA secondary elements has increased the structural diversity of DNA well beyond the ubiquitous double helix. However, it remains to be determined whether tertiary interactions can take place in a DNA complex that contains more than one secondary structure. Using a new data analysis strategy that exploits the hysteresis region between the mechanical unfolding and refolding traces obtained by a laser-tweezers instrument, we now provide the first convincing kinetic and thermodynamic evidence that a higher order interaction takes place between a hairpin and a G-quadruplex in a single-stranded DNA fragment that is found in the promoter region of human telomerase. During the hierarchical unfolding or refolding of the DNA complex, a 15-nucleotide hairpin serves as a common species among three intermediates. Moreover, either a mutant that prevents this hairpin formation or the addition of a DNA fragment complementary to the hairpin destroys the cooperative kinetic events by removing the tertiary interaction mediated by the hairpin. The coexistence of the sequential and the cooperative refolding events provides direct evidence for a unifying kinetic partition mechanism previously observed only in large proteins and complex RNA structures. Not only does this result rationalize the current controversial observations for the long-range interaction in complex single-stranded DNA structures, but also this unexpected complexity in a promoter element provides additional justification for the biological function of these structures in cells.
authors.affiliation: Department of Chemistry and Biochemistry and School of Biomedical Sciences, Kent State University, Kent, Ohio 44242, United States.
authors.forename: Zhongbo
authors.initials: Z
authors.lastname: Yu
authors.forename: Vanessa
authors.initials: V
authors.lastname: Gaerig
authors.forename: Yunxi
authors.initials: Y
authors.lastname: Cui
authors.forename: HyunJin
authors.initials: H
authors.lastname: Kang
authors.forename: Vijay
authors.initials: V
authors.lastname: Gokhale
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Laurence H
authors.initials: LH
authors.lastname: Hurley
authors.forename: Hanbin
authors.initials: H
authors.lastname: Mao
mesh_terms: D004247:DNA / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D009690:Nucleic Acid Conformation; D011401:Promoter Regions, Genetic; D017510:Protein Folding; D019098:Telomerase / Q000737:chemistry* / Q000378:metabolism; D013816:Thermodynamics
chemical_list: D004247:DNA; C509186:TERT protein, human; D019098:Telomerase