title: Increased osteoblast adhesion on physically optimized KRSR modified calcium aluminate.
abstract: Calcium aluminate (CA) is a porous biocompatible material easily cast at room temperature. Through this casting process, the average surface pore size of CA was varied from an average of 100 to 290 microns. The optimal surface pore size of the hydrated CA for cell viability was determined to be 100 microns. Further, a three step-solution deposition technique was developed to covalently immobilize cell adhesion peptides, RGD, and KRSR to the CA surface. Cell adhesion for 1-, 4-, and 7-day time periods was tested with primary osteoblasts and NIH 3T3 fibroblasts. Both peptides were found to increase fibroblast adhesion to the CA surface. However, only KRSR increased osteoblast adhesion to the surface of the CA, which may aid in bone formation after implantation.
authors.affiliation: Department of Chemistry and Biochemistry, Duquesne University, Pittsburgh, Pennsylvania 15282, USA.
authors.forename: Rachelle N
authors.initials: RN
authors.lastname: Palchesko
authors.forename: Jared D
authors.initials: JD
authors.lastname: Romeo
authors.forename: Kenneth A
authors.initials: KA
authors.lastname: McGowan
authors.forename: Ellen S
authors.initials: ES
authors.lastname: Gawalt
mesh_terms: D017607:Aluminum Compounds / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D017610:Calcium Compounds / Q000737:chemistry / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002648:Child; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006801:Humans; D008422:Materials Testing; D051379:Mice; D041681:NIH 3T3 Cells; D009842:Oligopeptides / Q000737:chemistry / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects* / Q000648:ultrastructure; D016062:Porosity / Q000187:drug effects; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D009842:Oligopeptides; C437191:lysyl-arginyl-seryl-arginine; C035219:calcium aluminate