title: Probe sialidase substrate specificity using chemoenzymatically synthesized sialosides containing C9-modified sialic acid.
abstract: A library of α2-3- and α2-6-linked sialyl galactosides containing C9-modified sialic acids was synthesized from C6-modified mannose derivatives using an efficient one-pot three-enzyme system. These sialosides were used in a high-throughput sialidase substrate specificity assay to elucidate the importance of C9-OH in sialidase recognition.
authors.affiliation: Department of Chemistry, University of California, One Shields Avenue, Davis, California 95616, USA.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Khedri
authors.forename: Musleh M
authors.initials: MM
authors.lastname: Muthana
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Li
authors.forename: Saddam M
authors.initials: SM
authors.lastname: Muthana
authors.forename: Hai
authors.initials: H
authors.lastname: Yu
authors.forename: Hongzhi
authors.initials: H
authors.lastname: Cao
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D005697:Galactosides / Q000737:chemistry*; D057166:High-Throughput Screening Assays; D006801:Humans; D008358:Mannose / Q000031:analogs & derivatives / Q000737:chemistry; D009439:Neuraminidase / Q000737:chemistry*; D012794:Sialic Acids / Q000737:chemistry*; D012799:Sialyltransferases / Q000737:chemistry*; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D005697:Galactosides; D012794:Sialic Acids; D012799:Sialyltransferases; D009439:Neuraminidase; D008358:Mannose