title: Silencing TRPC1 expression inhibits invasion of CNE2 nasopharyngeal             tumor cells.
abstract: The invasion and metastasis processes involved in nasopharyngeal carcinoma             (NPC) remain enigmatic. Transient receptor potential channel-related protein 1             (TRPC1) is a cation channel involved in diverse cellular functions by precisely             controlling Ca2+. The role of this unique TRPC member in nasopharyngeal malignancies             has not yet been delineated. Here, we downregulated TRPC1 in CNE2 cells by RNAi             technology and by using 2-APB, an inhibitor of the inositol 1,4,5-trisphosphate             (IP3) receptor and of store-operated Ca2+ channel-mediated Ca2+ entry. Both types             of TRPC1 inhibition resulted in significantly attenuated adhesive and invasive             abilities, suggesting that TRPC1 can modulate the metastasis of NPC. These findings             support further investigation of the potential of TRPC1 as a novel therapeutic             target for intervention in nasopharyngeal carcinoma.
authors.affiliation: Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, PR China.
authors.forename: Benfu
authors.initials: B
authors.lastname: He
authors.forename: Feiye
authors.initials: F
authors.lastname: Liu
authors.forename: Jian
authors.initials: J
authors.lastname: Ruan
authors.forename: Aimin
authors.initials: A
authors.lastname: Li
authors.forename: Jinzhang
authors.initials: J
authors.lastname: Chen
authors.forename: Rong
authors.initials: R
authors.lastname: Li
authors.forename: Jie
authors.initials: J
authors.lastname: Shen
authors.forename: Dayong
authors.initials: D
authors.lastname: Zheng
authors.forename: Rongcheng
authors.initials: R
authors.lastname: Luo
mesh_terms: D002277:Carcinoma; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000473:pathology*; D009361:Neoplasm Invasiveness; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D050052:TRPC Cation Channels / Q000235:genetics*
chemical_list: D034741:RNA, Small Interfering; D050052:TRPC Cation Channels; C096351:transient receptor potential cation channel, subfamily C, member 1; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9