title: Brevibacterium daeguense sp. nov., a nitrate-reducing bacterium isolated from a 4-chlorophenol enrichment culture.
abstract: A Gram-reaction-positive, non-spore-forming, aerobic actinobacterial strain (2C6-41(T)) was isolated from the activated sludge from an industrial wastewater treatment plant in Daegu, South Korea. Its taxonomic position was investigated by using a polyphasic approach. On the basis of 16S rRNA gene sequence similarity, closest phylogenetic relatives to strain 2C6-41(T) were Brevibacterium pityocampae DSM 21720(T) (97.2 %), Brevibacterium salitolerans KCTC 19616(T) (96.7 %), Brevibacterium album KCTC 19173(T) (96.2 %) and Brevibacterium samyangense KCCM 42316(T) (96.2 %). The DNA G+C content of strain 2C6-41(T) was 66.4 mol%. Chemotaxonomic data, which included MK-8(H(2)) as the major menaquinone; meso-diaminopimelic acid, glutamic acid and alanine as cell-wall amino acids; ribose, mannose and glucose as major cell-wall sugars; and anteiso-C(15 : 0), anteiso-C(17 : 0), C(16 : 0) and iso-C(15 : 0) as major fatty acids, supported the affiliation of strain 2C6-41(T) to the genus Brevibacterium. The aromatic ring cleavage enzyme catechol 1,2-dioxygenase was not detected in strain 2C6-41(T), but catechol 2,3-dioxygenase was detected. The results of physiological and biochemical tests, and the low level of DNA-DNA relatedness to the closest phylogenetic relative enabled strain 2C6-41(T) to be differentiated genotypically and phenotypically from recognized species of the genus Brevibacterium. The isolate is therefore considered to represent a novel species in the genus Brevibacterium, for which the name Brevibacterium daeguense sp. nov. is proposed. The type strain is 2C6-41(T) (=KCTC 19800(T) = JCM 17458(T)).
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1, Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors.forename: Yingshun
authors.initials: Y
authors.lastname: Cui
authors.affiliation: National Institute of Biological Resources, Environmental Research Complex, Gyeongseo-dong, Seo-gu, Incheon 404-708, Republic of Korea.
authors.forename: Myung-Suk
authors.initials: MS
authors.lastname: Kang
authors.affiliation: Research and Development Division, H-Plus Eco Ltd, BVC #301, KRIBB, Eoeun-dong, Yuseong-gu, Daejeon 305-333, Republic of Korea.|School of Civil and Environmental Engineering, Yonsei University, Seoul 120-749, Republic of Korea.
authors.forename: Sung-Geun
authors.initials: SG
authors.lastname: Woo
authors.affiliation: Environmental Biotechnology Research Center, Korea Research Institute of Bioscience & Biotechnology, 52 Eoeun-dong, Yuseong-gu, Daejeon 305-806, Republic of Korea.
authors.forename: Long
authors.initials: L
authors.lastname: Jin
authors.affiliation: Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, 52 Eoeun-dong, Yuseong-gu, Daejeon 305-806, Republic of Korea.
authors.forename: Kwang Kyu
authors.initials: KK
authors.lastname: Kim
authors.affiliation: School of Civil and Environmental Engineering, Yonsei University, Seoul 120-749, Republic of Korea.
authors.forename: Joonhong
authors.initials: J
authors.lastname: Park
authors.affiliation: Research and Development Division, H-Plus Eco Ltd, BVC #301, KRIBB, Eoeun-dong, Yuseong-gu, Daejeon 305-333, Republic of Korea.
authors.forename: Myungjin
authors.initials: M
authors.lastname: Lee
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1, Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors.forename: Sung-Taik
authors.initials: ST
authors.lastname: Lee
mesh_terms: D000596:Amino Acids / Q000032:analysis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D001951:Brevibacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D002241:Carbohydrates / Q000032:analysis; D002733:Chlorophenols; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009566:Nitrates / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D000596:Amino Acids; D002241:Carbohydrates; D002733:Chlorophenols; D004269:DNA, Bacterial; D005227:Fatty Acids; D009566:Nitrates; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C029107:4-chlorophenol; C024223:vitamin MK 8