title: [Expression of receptor activator nuclear factor kappa B ligand and osteoprotegerin in peri-implant soft tissue and bone tissue].
abstract: OBJECTIVE
  To study mRNA expression of receptor activator nuclear factor kappa B ligand (RANKL) and its decoy receptor, osteoprotegerin (OPG) in peri-implant tissue during unloading period.
  
  
  METHODS
  An animal model of dental implant was established in 6 male Beagle dogs of 1-2 years old. Bone remodeling was tested at 3, 7, 15, 30, 60 and 90 days since the placement of implants. RANKL and OPG mRNA expression were quantified by real-time polymerase chain reaction (PCR). Then mandibular bones were taken out and the morphological changes were observed by X-ray, bone tissue was tested by immunohistochemistry stain.
  
  
  RESULTS
  The most prominent period of bone remodeling occurred at 7th day after the placement of implants. The expression of RANKL and OPG increased in a time-dependent manner in both soft and hard tissue. After 7 days they gradually decreased.
  
  
  CONCLUSIONS
  RANKL and OPG can express in soft tissue, and the changing tendency is consistent with the change of bone remodeling, it indicates that RANKL and OPG play an important role in the bone remodeling.
authors.affiliation: Dental Implantation Centre, Stomatological Hospital of Yantai, Yantai 264008, China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Zhou
authors.forename: Zhonghao
authors.initials: Z
authors.lastname: Liu
authors.forename: Sheng
authors.initials: S
authors.lastname: Xu
authors.forename: Pengjie
authors.initials: P
authors.lastname: Hao
authors.forename: Fengwei
authors.initials: F
authors.lastname: Xu
authors.forename: Aijie
authors.initials: A
authors.lastname: Sun
authors.forename: Zhishan
authors.initials: Z
authors.lastname: Lu
mesh_terms: D000818:Animals; D016723:Bone Remodeling; D001842:Bone and Bones; D002352:Carrier Proteins; D004285:Dogs; D008297:Male; D016328:NF-kappa B; D053244:Osteoprotegerin; D053245:RANK Ligand; D053246:Receptor Activator of Nuclear Factor-kappa B
chemical_list: D002352:Carrier Proteins; D016328:NF-kappa B; D053244:Osteoprotegerin; D053245:RANK Ligand; D053246:Receptor Activator of Nuclear Factor-kappa B