title: Promotion of glycerol utilization using ethanol and 1-propanol in Schizosaccharomyces pombe.
abstract: The fission yeast Schizosaccharomyces pombe does not grow in media containing glycerol as a sole carbon source but uses glycerol in the presence of ethanol. Ethanol, but not glycerol, triggered upregulation of gld1+ and fbp1+ during glucose starvation even though gld1+ and fbp1+ are essential for growth on glycerol. This upregulation occurred at a very low concentration of ethanol. The transcriptional regulation of gld1+ was tested in the presence of various alcohols, and both ethanol and 1-propanol were found to induce gld1+ and to support growth in glycerol-containing media. We suggest that S. pombe has a novel ethanol and/or 1-propanol recognition mechanism that upregulates glycerol utilization during glucose starvation.
authors.affiliation: Department of Bioscience & Biotechnology, Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Fukuoka, Japan.
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Matsuzawa
authors.forename: Futoshi
authors.initials: F
authors.lastname: Hara
authors.forename: Hideki
authors.initials: H
authors.lastname: Tohda
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Uemura
authors.forename: Kaoru
authors.initials: K
authors.lastname: Takegawa
mesh_terms: D000433:1-Propanol / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D000431:Ethanol / Q000378:metabolism*; D006597:Fructose-Bisphosphatase / Q000096:biosynthesis; D015870:Gene Expression; D015966:Gene Expression Regulation, Fungal; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) / Q000096:biosynthesis; D005990:Glycerol / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D014158:Transcription, Genetic; D015854:Up-Regulation
chemical_list: D003470:Culture Media; D000431:Ethanol; D002244:Carbon; D000433:1-Propanol; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating); D006597:Fructose-Bisphosphatase; D005990:Glycerol