title: Biomembrane interactions reveal the mechanism of action of surface-immobilized host defense IDR-1010 peptide.
abstract: Dissecting the mechanism of action of surface-tethered antimicrobial and immunomodulatory peptides is critical to the design of optimized anti-infection coatings on biomedical devices. To address this, we compared the biomembrane interactions of host defense peptide IDR-1010cys (1) in free form, (2) as a soluble polymer conjugate, and (3) with one end tethered to a solid support with model bacterial and mammalian lipid membranes. Our results show that IDR-1010cys in all three distinct forms interacted with bacterial and mammalian lipid vesicles, but the extent of the interactions as monitored by the induction of secondary structure varied. The enhanced interaction of surface-tethered peptides is well correlated with their very good antimicrobial activities. Our results demonstrate that there may be a difference in the mechanism of action of surface-tethered versus free IDR-1010cys.
authors.affiliation: Centre for Blood Research, University of British Columbia, 2350 Health Sciences Mall, Vancouver, British Columbia V6T 1Z3, Canada.
authors.forename: Guangzheng
authors.initials: G
authors.lastname: Gao
authors.forename: John T J
authors.initials: JT
authors.lastname: Cheng
authors.forename: Jason
authors.initials: J
authors.lastname: Kindrachuk
authors.forename: Robert E W
authors.initials: RE
authors.lastname: Hancock
authors.forename: Suzana K
authors.initials: SK
authors.lastname: Straus
authors.forename: Jayachandran N
authors.initials: JN
authors.lastname: Kizhakkedathu
mesh_terms: D023181:Antimicrobial Cationic Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D055767:Immobilized Proteins / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism*; D008954:Models, Biological; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D011108:Polymers / Q000737:chemistry; D017433:Protein Structure, Secondary; D011791:Quartz / Q000737:chemistry; D013499:Surface Properties
chemical_list: D023181:Antimicrobial Cationic Peptides; D055767:Immobilized Proteins; D008051:Lipid Bilayers; D010455:Peptides; D011108:Polymers; D011791:Quartz