title: An investigation into the cytotoxic effects of 13-acetoxysarcocrassolide from the soft coral Sarcophyton crassocaule on bladder cancer cells.
abstract: Active compounds from natural products have been widely studied. The anti-tumor effects of 13-acetoxysarcocrassolide isolated from Formosan soft coral Sarcophyton crassocaule on bladder cancer cells were examined in this study. An MTT assay showed that 13-acetoxysarcocrassolide was cytotoxic to bladder female transitional cancer (BFTC) cells. We determined that the BFTC cells underwent cell death through apoptosis by flow cytometry. Due to the highly-migratory nature of the BFTC cells, the ability of 13-acetoxysarcocrassolide to stop their migration was assessed by a wound healing assay. To determine which proteins were affected in the BFTC cells upon treatment, a comparative proteomic analysis was performed. By LC-MS/MS analysis, we identified that 19 proteins were up-regulated and eight were down-regulated. Seven of the proteins were confirmed by western blotting analysis. This study reveals clues to the potential mechanism of the cytotoxic effects of 13-acetoxysarcocrassolide on BFTC cells. Moreover, it suggests that PPT1 and hnRNP F could be new biomarkers for bladder cancer. The results of this study are also helpful for the diagnosis, progression monitoring and therapeutic strategies of transitional cell tumors.
authors.affiliation: Antai Medical Care Cooperation Antai Tian-Sheng Memorial Hospital, Pingtung 92842, Taiwan.
authors.forename: Ching-Chyuan
authors.initials: CC
authors.lastname: Su
authors.affiliation: National Museum of Marine Biology and Aquarium, Pingtung 94446, Taiwan.
authors.forename: Jui-Hsin
authors.initials: JH
authors.lastname: Su
authors.affiliation: Department of Beauty Science, Meiho University, Pingtung 91202, Taiwan.
authors.forename: Jen-Jie
authors.initials: JJ
authors.lastname: Lin
authors.affiliation: Department of Beauty Science, Meiho University, Pingtung 91202, Taiwan.
authors.forename: Cheng-Chi
authors.initials: CC
authors.lastname: Chen
authors.affiliation: Department of Food Science and Nutrition, Meiho University, Pingtung 91202, Taiwan.
authors.forename: Wen-Ing
authors.initials: WI
authors.lastname: Hwang
authors.affiliation: Department of Beauty Science, Meiho University, Pingtung 91202, Taiwan.
authors.forename: Han Hsiang
authors.initials: HH
authors.lastname: Huang
authors.affiliation: Department of Beauty Science, Meiho University, Pingtung 91202, Taiwan.
authors.forename: Yu-Jen
authors.initials: YJ
authors.lastname: Wu
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000737:chemistry*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004224:Diterpenes / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D040901:Proteomics; D001749:Urinary Bladder Neoplasms / Q000188:drug therapy* / Q000473:pathology
chemical_list: C573067:13-acetoxysarcocrassolide; D000970:Antineoplastic Agents; D004224:Diterpenes