title: Potential protection of green tea polyphenols against intracellular amyloid beta-induced toxicity on primary cultured prefrontal cortical neurons of rats.
abstract: The present study was performed to explore the effect of green tea polyphenols on the intracellular Aβ (iAβ)-induced toxicity to cultured rat primary prefrontal cortical neurons. Administration of 100nM, 1μM or 10μM of green tea polyphenols significantly inhibited the iAβ-induced toxicity to cultured rat primary prefrontal cortical neurons tested by MTT and LDH release assays. We further studied the involvement of neuroprotective pathway protein AKT in green tea polyphenols protection against iAβ-induced cytotoxicity on cultured rat primary prefrontal cortical neurons. The results demonstrated that the content of p-AKT decreased significantly after iAβ treatment, while administration of green tea polyphenols significantly inhibited the iAβ-induced decrease in the content of p-AKT. Moreover, blockade of AKT signalling inhibited the protective effects of green tea polyphenols against iAβ-induced neurotoxicity. The results suggest that green tea polyphenols may play a protective effect on cultured rat primary prefrontal cortical neurons against iAβ-induced cytotoxicity and AKT is involved in the green tea polyphenols-induced protective effects.
authors.affiliation: Laboratory of Biotechnology and State Key Laboratory of Chinese Ethnic Minority Traditional Medicine, College of Life and Environmental Science, Minzu University of China, Beijing 100081, China.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Qin
authors.forename: Yong
authors.initials: Y
authors.lastname: Cheng
authors.forename: Long-Chuan
authors.initials: LC
authors.lastname: Yu
mesh_terms: D016229:Amyloid beta-Peptides / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D059808:Polyphenols / Q000494:pharmacology*; D017397:Prefrontal Cortex / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013662:Tea
chemical_list: D016229:Amyloid beta-Peptides; D018696:Neuroprotective Agents; D010936:Plant Extracts; D059808:Polyphenols; D013662:Tea