title: Targeted proteomics of the eicosanoid biosynthetic pathway completes an integrated genomics-proteomics-metabolomics picture of cellular metabolism.
abstract: Eicosanoids constitute a diverse class of bioactive lipid mediators that are produced from arachidonic acid and play critical roles in cell signaling and inflammatory aspects of numerous diseases. We have previously quantified eicosanoid metabolite production in RAW264.7 macrophage cells in response to Toll-like receptor 4 signaling and analyzed the levels of transcripts coding for the enzymes involved in the eicosanoid metabolite biosynthetic pathways. We now report the quantification of changes in protein levels under similar experimental conditions in RAW264.7 macrophages by multiple reaction monitoring mass spectrometry, an accurate targeted protein quantification method. The data complete the first fully integrated genomic, proteomic, and metabolomic analysis of the eicosanoid biochemical pathway.
authors.affiliation: Department of Biology, Institute of Molecular Systems Biology and Competence Center for Systems Physiology and Metabolic Disease, ETH Zurich, 8093 Zurich, Switzerland.
authors.forename: Eduard
authors.initials: E
authors.lastname: Sabidó
authors.forename: Oswald
authors.initials: O
authors.lastname: Quehenberger
authors.forename: Qin
authors.initials: Q
authors.lastname: Shen
authors.forename: Ching-Yun
authors.initials: CY
authors.lastname: Chang
authors.forename: Ishita
authors.initials: I
authors.lastname: Shah
authors.forename: Aaron M
authors.initials: AM
authors.lastname: Armando
authors.forename: Alexander
authors.initials: A
authors.lastname: Andreyev
authors.forename: Olga
authors.initials: O
authors.lastname: Vitek
authors.forename: Edward A
authors.initials: EA
authors.lastname: Dennis
authors.forename: Ruedi
authors.initials: R
authors.lastname: Aebersold
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism*; D053898:Biosynthetic Pathways / Q000187:drug effects*; D002460:Cell Line; D015777:Eicosanoids / Q000096:biosynthesis*; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism*; D050356:Lipid Metabolism / Q000187:drug effects; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D013058:Mass Spectrometry; D055432:Metabolomics; D051379:Mice; D040901:Proteomics; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D015777:Eicosanoids; C506188:Kdo2-lipid A; D008070:Lipopolysaccharides; D016718:Arachidonic Acid; D000255:Adenosine Triphosphate