title: Total steroidal alkaloids from Veratrum patulum L. Inhibit platelet aggregation, thrombi formation and decrease bleeding time in rats.
abstract: BACKGROUND
  Veratrum patulum L. (Liliaceae) is used as one source of the Chinese traditional drug "Lilu" which has been used in the treatment of aphasia arising from apoplexy, wind type dysentery, headache, etc. for thousands of years.
  
  
  OBJECTIVE
  To evaluate the antithrombotic effect of Veratrum patulum L. (Liliaceae) total steroidal alkaloids (VpA) and explore the potential mechanisms.
  
  
  METHODS
  The antithrombotic effect of VpA was evaluated in two experimental thrombosis models in rat. The potential antithrombotic mechanisms of VpA were explored by determining the coagulation parameters and platelet aggregation induced by various agonists. Cutting-tail rat model was used to evaluate the influence of VpA on bleeding time.
  
  
  RESULTS
  VpA significantly inhibit the formation of arterial and venous thrombosis and showed different inhibitory effect on the platelet aggregation induced by various agonists with the following potency order: collagen>ADP>thrombin. VpA showed no influence on the coagulation parameters in rat. But VpA decreased the cutting-tail bleeding time in rat.
  
  
  CONCLUSIONS
  VpA possess antithrombotic effect on venous and arterial thrombosis. The antithrombotic effect of VpA is due to its inhibition to platelet aggregation, especially induced by collagen.
authors.affiliation: School of Pharmaceutical Science and Technology, Dalian University of Technology, No. 2 Linggong Road, Ganjingzi District, Dalian City, Liaoning Province 116024, PR China.
authors.forename: Qiling
authors.initials: Q
authors.lastname: Song
authors.forename: Shisheng
authors.initials: S
authors.lastname: Wang
authors.forename: Weijie
authors.initials: W
authors.lastname: Zhao
mesh_terms: D000244:Adenosine Diphosphate; D000818:Animals; D000925:Anticoagulants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001760:Bleeding Time; D001777:Blood Coagulation / Q000187:drug effects*; D003094:Collagen; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D005343:Fibrinolytic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D008517:Phytotherapy; D018517:Plant Roots; D010946:Plants, Medicinal; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010979:Platelet Function Tests; D051381:Rats; D017207:Rats, Sprague-Dawley; D027343:Rhizome; D013917:Thrombin; D013927:Thrombosis / Q000097:blood / Q000517:prevention & control*; D013997:Time Factors; D014703:Veratrum / Q000737:chemistry; D014704:Veratrum Alkaloids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000925:Anticoagulants; D004365:Drugs, Chinese Herbal; D005343:Fibrinolytic Agents; D010975:Platelet Aggregation Inhibitors; D014704:Veratrum Alkaloids; D000244:Adenosine Diphosphate; D003094:Collagen; D013917:Thrombin