title: (1)H, (13)C, and (15)N chemical shifts assignments for human endothelial monocyte-activating polypeptide EMAP II.
abstract: Endothelial and monocyte-activating polypeptide II (EMAP II) is a cytokine that plays an important role in inflammation, apoptosis and angiogenesis processes in tumour tissues. Structurally, the EMAP II is a 169 amino acid residues long C-terminal domain (residues 147-312) of auxiliary tRNA binding protein p43. In spite of existence in pdb databank of two X-ray structures there are some important aspects of EMAP II cytokine function which are still not fully understood in detail. To obtain information about 3D structure and backbone dynamic processes in solution we perform structure evaluation of human EMAP II cytokine by NMR spectroscopy. The standard approach to sequence-specific backbone assignment using 3D NMR data sets was not successful in our studies and was supplemented by recently developed 4D NMR experiments with random sampling of evolution time space. Here we report the backbone and side chain (1)H, (13)C, and (15)N chemical shifts in solution for recombinant EMAP II cytokine together with secondary structure provided by TALOS + software.
authors.affiliation: Institute Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Acad. Zabolotnogo 150, 03-143, Kyiv, Ukraine.
authors.forename: Dmytro
authors.initials: D
authors.lastname: Lozhko
authors.forename: Jan
authors.initials: J
authors.lastname: Stanek
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Kazimierczuk
authors.forename: Anna
authors.initials: A
authors.lastname: Zawadzka-Kazimierczuk
authors.forename: Wiktor
authors.initials: W
authors.lastname: Kozminski
authors.forename: Igor
authors.initials: I
authors.lastname: Zhukov
authors.forename: Alexander
authors.initials: A
authors.lastname: Kornelyuk
mesh_terms: D000595:Amino Acid Sequence; D016207:Cytokines / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D011487:Protein Conformation; D016601:RNA-Binding Proteins / Q000737:chemistry*; D012996:Solutions
chemical_list: D016207:Cytokines; D009363:Neoplasm Proteins; D016601:RNA-Binding Proteins; D012996:Solutions; C077219:small inducible cytokine subfamily E, member 1