title: Structure of the human metapneumovirus fusion protein with neutralizing antibody identifies a pneumovirus antigenic site.
abstract: Human metapneumovirus and respiratory syncytial virus cause lower respiratory tract infections. The virus fusion (F) glycoprotein promotes membrane fusion by refolding from a metastable pre-fusion to a stable post-fusion conformation. F is also a major target of the neutralizing antibody response. Here we show that a potently neutralizing anti-human metapneumovirus antibody (DS7) binds a structurally invariant domain of F, revealing a new epitope that could be targeted in vaccine development.
authors.affiliation: Department of Structural Biology, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Wen
authors.forename: Jens C
authors.initials: JC
authors.lastname: Krause
authors.forename: George P
authors.initials: GP
authors.lastname: Leser
authors.forename: Reagan G
authors.initials: RG
authors.lastname: Cox
authors.forename: Robert A
authors.initials: RA
authors.lastname: Lamb
authors.forename: John V
authors.initials: JV
authors.lastname: Williams
authors.forename: James E
authors.initials: JE
authors.lastname: Crowe
authors.forename: Theodore S
authors.initials: TS
authors.lastname: Jardetzky
mesh_terms: D057134:Antibodies, Neutralizing / Q000737:chemistry* / Q000276:immunology; D000939:Epitopes / Q000737:chemistry* / Q000276:immunology; D006801:Humans; D029121:Metapneumovirus / Q000737:chemistry* / Q000276:immunology; D008958:Models, Molecular; D018184:Paramyxoviridae Infections / Q000276:immunology / Q000821:virology; D017434:Protein Structure, Tertiary; D014760:Viral Fusion Proteins / Q000737:chemistry* / Q000276:immunology
chemical_list: D057134:Antibodies, Neutralizing; D000939:Epitopes; D014760:Viral Fusion Proteins