title: Quantitative proteomics study on the protective mechanism of phlorizin             on hepatic damage in diabetic db/db mice.
abstract: Although phlorizin has been used in the treatment of diabetes mellitus for             over 100 years, the underlying molecular mechanisms have not been fully elucidated.             This study investigated the effect of phlorizin on body weight, blood glucose,             blood triglycerides (TG), blood total cholesterol (TC), as well as overall changes             in protein expression in db/db diabetic mouse liver. Phlorizin significantly decreased             body weight gain and the levels of glucose, TC and TG in blood. Isobaric tag for             relative and absolute quantitation (iTRAQ) quantitative proteomics profiling revealed             that phlorizin interfered with the processes of carbohydrate metabolism, fatty             acid biosynthesis and β-oxidation, cholesterol biosynthesis, and free radical             scavenging by affecting the expression of key proteins in these processes. Ingenuity             Pathway Analysis successfully established several pathway networks, in which many             differentially expressed proteins were involved. The differential expression of             several proteins was validated by western blotting. Our study offers important             information on the mechanism of phlorizin treatment in diabetes mellitus, particularly             in the liver.
authors.affiliation: Key Laboratory of Cardiovascular Proteomics of Shandong University, Department of Geriatrics, Qi-Lu Hospital of Shandong University, Jinan, Shandong, PR China.
authors.forename: Wei-Da
authors.initials: WD
authors.lastname: Lu
authors.forename: Bao-Ying
authors.initials: BY
authors.lastname: Li
authors.forename: Fei
authors.initials: F
authors.lastname: Yu
authors.forename: Qian
authors.initials: Q
authors.lastname: Cai
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
authors.forename: Mei
authors.initials: M
authors.lastname: Yin
authors.forename: Hai-Qing
authors.initials: HQ
authors.lastname: Gao
mesh_terms: D000818:Animals; D050260:Carbohydrate Metabolism / Q000187:drug effects*; D003920:Diabetes Mellitus / Q000378:metabolism* / Q000473:pathology; D005786:Gene Expression Regulation / Q000187:drug effects; D050356:Lipid Metabolism / Q000187:drug effects*; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008817:Mice, Mutant Strains; D010695:Phlorhizin / Q000494:pharmacology*; D040901:Proteomics / Q000379:methods
chemical_list: D010695:Phlorhizin