title: Biodegradation of kraft lignin by a bacterial strain Comamonas sp. B-9 isolated from eroded bamboo slips.
abstract: OBJECTIVE
  The aim was to obtain evidences for lignin degradation by unicellular bacterium Comamonas sp. B-9.
  
  
  RESULTS
  Comamonas sp. B-9 was inoculated into kraft lignin-mineral salt medium (KL-MSM) at pH 7·0 and 30°C for 7 days of incubation. The bacterial growth, chemical oxygen demand (COD) reduction, secretion of ligninolytic enzymes and productions of low-molecular-weight compounds revealed that Comamonas sp. B-9 was able to degrade kraft lignin (KL). COD in KL-MSM reduced by 32% after 7 days of incubation. The maximum activities of manganese peroxidase (MnP) of 2903·2 U l(-1) and laccase (Lac) of 1250 U l(-1) were observed at 4th and 6th day, respectively. The low-molecular-weight compounds such as ethanediol, 3, 5-dimethyl-benzaldehyde and phenethyl alcohol were formed in the degradation of KL by Comamonas sp. B-9 based on GC-MS analysis.
  
  
  CONCLUSIONS
  This study confirmed that Comamonas sp. B-9 could utilize KL as a sole carbon source and degrade KL to low-molecular-weight compounds.
  
  
  CONCLUSIONS
  Comamonas sp. B-9 may be useful in the utilization and bioconversion of lignin and lignin-derived aromatic compounds in biotechnological applications. Meanwhile, using Comamonas sp. B-9 in treatment of wastewater in pulp and paper industry is a meaningful work.
authors.affiliation: School of Metallurgical Science and Engineering, Central South University, Changsha, China.
authors.forename: Y H
authors.initials: YH
authors.lastname: Chen
authors.forename: L Y
authors.initials: LY
authors.lastname: Chai
authors.forename: Y H
authors.initials: YH
authors.lastname: Zhu
authors.forename: Z H
authors.initials: ZH
authors.lastname: Yang
authors.forename: Y
authors.initials: Y
authors.lastname: Zheng
authors.forename: H
authors.initials: H
authors.lastname: Zhang
mesh_terms: D031723:Bambusa / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002244:Carbon / Q000378:metabolism; D020585:Comamonas / Q000145:classification / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D006863:Hydrogen-Ion Concentration; D042845:Laccase / Q000378:metabolism; D008031:Lignin / Q000378:metabolism*; D008969:Molecular Sequence Data; D010209:Paper; D010544:Peroxidases / Q000378:metabolism
chemical_list: D002244:Carbon; C076151:Kraft lignin; D008031:Lignin; D042845:Laccase; D010544:Peroxidases; C051129:manganese peroxidase