title: [Endothelial dysfunction induced by high glucose is associated with decreased ATP-binding cassette transporter G1 expression].
abstract: OBJECTIVE
  To investigate the role of ATP-binding cassette transporter G1 (ABCG1) in endothelial dysfunction induced by high glucose.
  
  
  METHODS
  Human aortic endothelial cells (HAECs) were incubated in the presence of 5.6 or 30 mmol/L glucose for 24-72 h with or without a 2-h pretreatment with the LXR agonist 22(R)-hydroxycholesterol. Real-time PCR and Western blotting were used to measure the mRNA and protein expressions of ABCG1; the intracellular cholesterol efflux and endothelial nitric oxide synthase (eNOS) activity were measured by scintillation counting.
  
  
  RESULTS
  High glucose time-dependently suppressed ABCG1 expression and cholesterol efflux to HDL in HAECs. High glucose also decreased eNOS activity. ABCG1 down-regulation induced by high glucose, along with decreased cholesterol efflux and eNOS activity, was abolished by treatment of the cells with the LXR agonist.
  
  
  CONCLUSIONS
  Endothelial dysfunction induced by high glucose is associated with decreased ABCG1 expression.
authors.affiliation: Department of Cardiovascular Medicine, Xi'an Jiaotong University College of Medicine, Xi'an, China. xjh1224@163.com
authors.forename: Jiahong
authors.initials: J
authors.lastname: Xue
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Niu
authors.forename: Jin
authors.initials: J
authors.lastname: Wei
authors.forename: Xin
authors.initials: X
authors.lastname: Dong
authors.forename: Canzhan
authors.initials: C
authors.lastname: Zhu
authors.forename: Yinhu
authors.initials: Y
authors.lastname: Dang
authors.forename: Anqi
authors.initials: A
authors.lastname: Song
authors.forename: Huimei
authors.initials: H
authors.lastname: Huang
mesh_terms: D000070998:ATP Binding Cassette Transporter, Subfamily G, Member 1; D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D001011:Aorta / Q000166:cytology*; D002460:Cell Line; D015536:Down-Regulation / Q000187:drug effects; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D005947:Glucose / Q000494:pharmacology*; D006801:Humans
chemical_list: C419353:ABCG1 protein, human; D000070998:ATP Binding Cassette Transporter, Subfamily G, Member 1; D018528:ATP-Binding Cassette Transporters; D005947:Glucose