title: The clathrin assembly protein PICALM is required for erythroid maturation and transferrin internalization in mice.
abstract: Phosphatidylinositol binding clathrin assembly protein (PICALM), also known as clathrin assembly lymphoid myeloid leukemia protein (CALM), was originally isolated as part of the fusion gene CALM/AF10, which results from the chromosomal translocation t(10;11)(p13;q14). CALM is sufficient to drive clathrin assembly in vitro on lipid monolayers and regulates clathrin-coated budding and the size and shape of the vesicles at the plasma membrane. However, the physiological role of CALM has yet to be elucidated. Here, the role of CALM in vivo was investigated using CALM-deficient mice. CALM-deficient mice exhibited retarded growth in utero and were dwarfed throughout their shortened life-spans. Moreover, CALM-deficient mice suffered from severe anemia, and the maturation and iron content in erythroid precursors were severely impaired. CALM-deficient erythroid cells and embryonic fibroblasts exhibited impaired clathrin-mediated endocytosis of transferrin. These results indicate that CALM is required for erythroid maturation and transferrin internalization in mice.
authors.affiliation: Department of Biological Science, Graduate School of Humanities and Sciences, Nara Women's University, Nara, Japan.
authors.forename: Mai
authors.initials: M
authors.lastname: Suzuki
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Tanaka
authors.forename: Akira
authors.initials: A
authors.lastname: Tanimura
authors.forename: Kenji
authors.initials: K
authors.lastname: Tanabe
authors.forename: Natsuko
authors.initials: N
authors.lastname: Oe
authors.forename: Shinya
authors.initials: S
authors.lastname: Rai
authors.forename: Syunsuke
authors.initials: S
authors.lastname: Kon
authors.forename: Manabu
authors.initials: M
authors.lastname: Fukumoto
authors.forename: Kohji
authors.initials: K
authors.lastname: Takei
authors.forename: Takaya
authors.initials: T
authors.lastname: Abe
authors.forename: Itaru
authors.initials: I
authors.lastname: Matsumura
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Kanakura
authors.forename: Toshio
authors.initials: T
authors.lastname: Watanabe
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000378:metabolism*; D000740:Anemia / Q000473:pathology; D000818:Animals; D002454:Cell Differentiation; D003433:Crosses, Genetic; D004622:Embryo, Mammalian / Q000166:cytology; D004705:Endocytosis; D004900:Erythroblasts / Q000166:cytology / Q000378:metabolism; D041905:Erythroid Cells / Q000166:cytology* / Q000378:metabolism*; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D005434:Flow Cytometry; D018390:Gene Targeting; D005838:Genotype; D006410:Hematopoiesis; D007501:Iron / Q000378:metabolism; D008136:Longevity; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D034041:Monomeric Clathrin Assembly Proteins; D014168:Transferrin / Q000378:metabolism*
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D034041:Monomeric Clathrin Assembly Proteins; C573094:PICALM protein, mouse; D014168:Transferrin; D007501:Iron