title: Involvement of Mcl1 in diallyl disulfide-induced G2/M cell cycle arrest             in HL-60 cells.
abstract: Diallyl disulfide (DADS) has shown potential as a therapeutic agent in various             cancers. Previously, we found that myeloid cell leukemia sequence 1 (Mcl1) was             downregulated in DADS-induced cell cycle arrest in HL-60 human leukemia cells.             Here, we investigated the role of this protein in DADS-induced G2/M cell cycle             arrest in HL-60 cells. We demonstrated that DADS treatment significantly increased             the proportion of G2/M phase HL-60 cells (P<0.05) and caused a time-dependent             significant downregulation of Mcl1 and the cell cycle-related proteins PCNA and             CDK1 (P<0.05). Small interfering RNA-mediated knockdown of Mcl1 expression             in HL-60 cells arrested the cell cycle in G2/M phase. By co-immunoprecipitation,             we demonstrated that Mcl1 associated with PCNA and CDK1 in G2/M cell cycle arrest             in DADS-treated HL-60 cells. DADS decreased the interaction of Mcl1 with PCNA             and CDK1, leading to G2/M cell cycle arrest in HL-60 cells. Mcl1 plays an important             role in DADS-induced G2/M cell cycle arrest in HL-60 human leukemia cells.
authors.affiliation: Cancer Research Institute, University of South China, Hengyang 421001, Hunan Province, PR China.
authors.forename: Lan
authors.initials: L
authors.lastname: Yi
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Ji
authors.forename: Hui
authors.initials: H
authors.lastname: Tan
authors.forename: Mei-Yan
authors.initials: MY
authors.lastname: Feng
authors.forename: Yi
authors.initials: Y
authors.lastname: Tang
authors.forename: Ling
authors.initials: L
authors.lastname: Wen
authors.forename: Qi
authors.initials: Q
authors.lastname: Su
mesh_terms: D000498:Allyl Compounds / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D016203:CDC2 Protein Kinase / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004220:Disulfides / Q000494:pharmacology*; D059565:G2 Phase Cell Cycle Checkpoints / Q000187:drug effects*; D018922:HL-60 Cells; D006801:Humans; D064549:Myeloid Cell Leukemia Sequence 1 Protein; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D034741:RNA, Small Interfering
chemical_list: D000498:Allyl Compounds; D000970:Antineoplastic Agents; D018797:Cell Cycle Proteins; D004220:Disulfides; C579095:MCL1 protein, human; D064549:Myeloid Cell Leukemia Sequence 1 Protein; D018809:Proliferating Cell Nuclear Antigen; D019253:Proto-Oncogene Proteins c-bcl-2; D034741:RNA, Small Interfering; C028009:diallyl disulfide; D016203:CDC2 Protein Kinase