title: Estimation of taurindicine  hybridization of American Zebu cattle in Brazil.
abstract: Our  objective was to estimate Bos primigenius taurus introgression in  American Zebu cattle. One hundred and four American Zebu (Nellore) cattle were  submitted to mtDNA, microsatellite and satellite analysis. Twenty-three alleles  were detected in microsatellite analysis, averaging 4.6 ± 1.82/locus. Variance  component comparisons of microsatellite allele sizes allowed the construction  of two clusters separating taurus and indicus. No significant  variation was observed when indicus and taurus mtDNA were  compared. Three possible genotypes of 1711b satellite DNA were identified. All  European animals showed the same restriction pattern, suggesting a  Zebu-specific restriction pattern. The frequencies of B. primigenius indicus-specific  microsatellite alleles and 1711b satellite DNA restriction patterns lead to an  estimate of 14% taurine contribution in purebred Nellore.
authors.affiliation: Departamento de Ciências Básicas, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, SP, Brasil.
authors.forename: P
authors.initials: P
authors.lastname: Ripamonte
authors.forename: M
authors.initials: M
authors.lastname: Baccaglini
authors.forename: A S M
authors.initials: AS
authors.lastname: Cesar
authors.forename: L G G
authors.initials: LG
authors.lastname: Figueiredo
authors.forename: J C C
authors.initials: JC
authors.lastname: Balieiro
authors.forename: A R
authors.initials: AR
authors.lastname: Caetano
authors.forename: F V
authors.initials: FV
authors.lastname: Meirelles
mesh_terms: D000483:Alleles; D000818:Animals; D001938:Brazil; D002417:Cattle / Q000145:classification / Q000235:genetics*; D002678:Chimera / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics; D004276:DNA, Satellite / Q000235:genetics*; D005787:Gene Frequency; D018895:Microsatellite Repeats / Q000235:genetics*; D015183:Restriction Mapping / Q000379:methods*
chemical_list: D004272:DNA, Mitochondrial; D004276:DNA, Satellite