title: Changes in thyroid hormone levels during zebrafish development.
abstract: The zebrafish (Danio rerio) has been used as a model for the study of endocrine disrupting chemicals. This study set out to determine the profiles of whole-body thyroxine (T4) and 3,5,3'-triiodothyronine (T3) levels during the development of zebrafish from embryo to adult. Enzyme-linked immunoassay was used to analyze whole-body T4 and T3 contents. The results showed that whole-body T4 and T3 levels remained stable during the pre-hatching period (0-3 d) and increased significantly during early development after hatching. The T3 level peaked at 0.28 ± 0.01 ng g(-1) body weight at 10 days post-fertilization (dpf), and T4 peaked at 0.58 ± 0.09 ng g(-1) body weight at 21 dpf. Both thyroid hormones subsequently declined during later development. This study establishes a baseline for thyroid hormones in zebrafish, which will be vital for the understanding of thyroid hormone functions and in future studies of thyroid toxicants in this species.
authors.affiliation: Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou, China.
authors.forename: Juhua
authors.initials: J
authors.lastname: Chang
authors.forename: Minghua
authors.initials: M
authors.lastname: Wang
authors.forename: Wenjun
authors.initials: W
authors.lastname: Gui
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhao
authors.forename: Liang
authors.initials: L
authors.lastname: Yu
authors.forename: Guonian
authors.initials: G
authors.lastname: Zhu
mesh_terms: D000818:Animals; D004625:Embryo, Nonmammalian / Q000378:metabolism; D013961:Thyroid Gland / Q000196:embryology; D013974:Thyroxine / Q000378:metabolism*; D013997:Time Factors; D014284:Triiodothyronine / Q000378:metabolism*; D015027:Zebrafish / Q000196:embryology*
chemical_list: D014284:Triiodothyronine; D013974:Thyroxine