title: [Simultaneous determination of four acids active compounds in Erodium stephanianum by RP-HPLC].
abstract: OBJECTIVE
  To establish a RP-HPLC method for the determination of four acids compounds including gallic acid, protocatechuic acid, corilagin and ellagic acid in Erodium Stephanianum.
  
  
  METHODS
  The RP-HPLC separation was performed on an Agilent TC-C18 analytical column (4.6 mm x 250 mm, 5 microm). The mobile phase was methanol (A) -water containing 0.4% H3PO4 (B) with gradient elution mode at the flow rate of 0.8 mL x min(-1). The detection wavelength was set at 259 nm, and the column temperature was 30 degrees C.
  
  
  RESULTS
  The liner ranges of gallic acid, protocatechuic acid, corilagin and ellagic acid were 0.059-2.360 g x L(-1) (r = 0.999 6), 0.017-0.672 g x L(-1) (r = 0.999 9), 0.351-14.040 g x L(-1) (r = 0.999 9), and 0.151-6.040 g x L(-1) (r = 0.999 8), respectively. The average recoveries (n = 3) were 99.45% (RSD 1.5%), 98.65% (RSD 1.7%), 100.3% (RSD 2.0%), and 98.90% (RSD 1.2%), respectively.
  
  
  CONCLUSIONS
  The method is simple and accurate with a good reproducibility and can be used for quality control of Erodium stephanianum.
authors.affiliation: Liaoning University of Traditional Chinese Medicine, Dalian 116600, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Zhao
authors.forename: Haibo
authors.initials: H
authors.lastname: Yin
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004610:Ellagic Acid / Q000032:analysis; D005707:Gallic Acid / Q000032:analysis; D031314:Geraniaceae / Q000737:chemistry*; D005960:Glucosides / Q000032:analysis; D047348:Hydrolyzable Tannins; D062385:Hydroxybenzoates / Q000032:analysis
chemical_list: D005960:Glucosides; D047348:Hydrolyzable Tannins; D062385:Hydroxybenzoates; D004610:Ellagic Acid; C009091:protocatechuic acid; C049096:corilagin; D005707:Gallic Acid