title: Effect of manassantin B, a lignan isolated from Saururus chinensis, on lipopolysaccharide-induced interleukin-1β in RAW 264.7 cells.
abstract: BACKGROUND
  Elevated systemic levels of pro-inflammatory cytokines cause hypotension during septic shock and induce capillary leakage in acute lung injury. Manassantin B has anti-inflammatory and anti-plasmoidal properties. This study examined the effects of manassantin B on lipopolysaccharide (LPS)-induced inflammatory response in murine macrophages.
  
  
  METHODS
  RAW 264.7 macrophage cells were incubated without or with (1, 3 and 10 µM) manassantin B and without or with (100 ng/ml) LPS. Manassantin B dissolved in phosphate buffered saline was added to the medium 1 h prior to the addition of LPS. The degree of activation of mitogen-activated protein kinase (MAPK) including extracellular signal-regulated kinases 1 and 2 (ERK1/2), c-Jun amino terminal kinases (JNK) and p38 MAPK, and the level of interleukin (IL)-1β were determined 30 min and 24 h after the addition of LPS respectively.
  
  
  RESULTS
  Manassantin B inhibited the production of IL-1β and attenuated the phosphorylations of ERK1/2 and p38 MAPK, but not that of JNK, in RAW 264.7 cells treated with LPS.
  
  
  CONCLUSIONS
  Manassantin B reduces LPS-induced IL-1β expression through effects on ERK1/2- and p38 MAPK-mediated pathways. Manassantin B has potential as a potent anti-inflammatory drug for use in pathological processes such as sepsis or acute lung injury.
authors.affiliation: Department of Anesthesiology and Pain Medicine, Chonnam Nationanl University Hospital, Gwangju, Korea.
authors.forename: Hwan Chul
authors.initials: HC
authors.lastname: Park
authors.forename: Hong-Beom
authors.initials: HB
authors.lastname: Bae
authors.forename: Cheol-Won
authors.initials: CW
authors.lastname: Jeong
authors.forename: Seong Heon
authors.initials: SH
authors.lastname: Lee
authors.forename: Hye Jin
authors.initials: HJ
authors.lastname: Jeung
authors.forename: Sang-Hyun
authors.initials: SH
authors.lastname: Kwak