title: Human respiratory epithelial cells from nasal turbinate expressed stem cell genes even after serial passaging.
abstract: Current development in the field of tissue engineering led to the idea of repairing and regenerating the respiratory airway through in vitro reconstruction using autologous respiratory epithelial (RE). To ensure the capability of proliferation, the stem cell property of RE cells from the nasal turbinate should be evaluated. Respiratory epithelial cells from six human nasal turbinates were harvested and cultured in vitro. The gene expression of FZD-9 and BST-1 were expressed in passage 2 (P2) and passage 4 (P4). The levels of expression were not significant between both passages. The RE cells exhibit the stem cell properties, which remains even after serial passaging.
authors.affiliation: Tissue Engineering Centre, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latif, Bandar Tun Razak, 56000 Kuala Lumpur, Malaysia. ruszyidrus@gmail.com
authors.forename: B H I
authors.initials: BH
authors.lastname: Ruszymah
authors.forename: B A Azrul
authors.initials: BA
authors.lastname: Izham
authors.forename: M Y Mohd
authors.initials: MY
authors.lastname: Heikal
authors.forename: S F
authors.initials: SF
authors.lastname: Khor
authors.forename: M B
authors.initials: MB
authors.lastname: Fauzi
authors.forename: B S
authors.initials: BS
authors.lastname: Aminuddin
mesh_terms: D036541:ADP-ribosyl Cyclase / Q000235:genetics / Q000378:metabolism; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism; D005434:Flow Cytometry; D051157:Frizzled Receptors / Q000235:genetics / Q000378:metabolism; D058851:GPI-Linked Proteins / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D009297:Nasal Mucosa / Q000166:cytology*; D060888:Real-Time Polymerase Chain Reaction; D023822:Tissue Engineering / Q000379:methods*; D014182:Transplantation, Autologous; D014420:Turbinates / Q000166:cytology*
chemical_list: D015703:Antigens, CD; C549560:FZD9 protein, human; D051157:Frizzled Receptors; D058851:GPI-Linked Proteins; D036541:ADP-ribosyl Cyclase; C087521:ADP-ribosyl cyclase 2