title: [Effect of sodium butyrate on apoptosis and stromal interaction molecule and Orai1 activity in human colon cancer HCT-116 cells in vitro].
abstract: OBJECTIVE
  To investigate the mechanism underlying sodium butyrate (NaB)-induced apoptosis of a human colon cancer cell line HCT-116.
  
  
  METHODS
  The apoptosis of HCT-116 cells induced by NaB was confirmed by hoechst33342 staining and AnnexinV+ PI assay. The changes in the intracellular localization of stromal interaction molecule (STIM1) and Orai1 following NaB treatment were detected by immunofluorescence technique. Western blotting was used to investigate the protein expression levels of STIM1 and Orai1.
  
  
  RESULTS
  NaB induced apoptosis and caused translocation and colocalization of STIM1 and Orai1 in HCT-116 cells.
  
  
  CONCLUSIONS
  The apoptosis of human colon cancer cells induced by NaB is correlated to the redistribution of STIM1 and Orai1.
authors.affiliation: Department of Nutrition and Food Hygiene, Southern Medical University, Guangzhou, China. sunsuxia@fimmu.com
authors.forename: Suxia
authors.initials: S
authors.lastname: Sun
authors.forename: Wenjun
authors.initials: W
authors.lastname: Li
authors.forename: Siqiang
authors.initials: S
authors.lastname: Chen
authors.forename: He
authors.initials: H
authors.lastname: Zhang
authors.forename: Shaozhen
authors.initials: S
authors.lastname: Yu
authors.forename: Minhong
authors.initials: M
authors.lastname: Zhang
authors.forename: Fei
authors.initials: F
authors.lastname: Zou
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002087:Butyrates / Q000494:pharmacology*; D015220:Calcium Channels / Q000378:metabolism*; D045325:HCT116 Cells; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000378:metabolism*; D000071740:ORAI1 Protein; D000071737:Stromal Interaction Molecule 1
chemical_list: D000970:Antineoplastic Agents; D002087:Butyrates; D015220:Calcium Channels; D056572:Histone Deacetylase Inhibitors; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D000071740:ORAI1 Protein; C510746:ORAI1 protein, human; C103530:STIM1 protein, human; D000071737:Stromal Interaction Molecule 1