title: [Optimization of a compound prescription for treating liver fibrosis].
abstract: OBJECTIVE
  To optimize a compound prescription for treatment of liver fibrosis with an improved therapeutic effect and low toxicity.
  
  
  METHODS
  In rat models of liver fibrosis induced by thioacetamide (TAA), the optimized prescription was screened based on a uniform design with 2-factor 5-level table using Uniform Design 3.0 software and tested using liver content of Hyp as the screening index. To verify the efficacy of the optimized prescription, the rat models of liver fibrosis were randomized  into normal control group, model group, colchicine group and optimized prescription group, and the changes of hepatic Hyp content, serum HA, ALT, AST, and ALB levels, and the pathology liver fibrosis were observed after corresponding treatments.
  
  
  RESULTS
  The optimized prescription, which contained 70 mg/kg glycyrrhizin and 70 mg/kg matrine, showed a significant therapeutic effect against liver fibrosis in rats (Plt;0.05), and the effect was equivalent to that of colchicine (P>0.05).
  
  
  CONCLUSIONS
  Uniform design is a valuable method in prescription optimization. The optimized compound prescription of matrine and glycyrrhizin has a significant effect in inhibiting liver fibrosis.
authors.affiliation: Department of Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China. 166987@qq.com
authors.forename: Liang
authors.initials: L
authors.lastname: Huang
authors.forename: Linyan
authors.initials: L
authors.lastname: Qi
authors.forename: Zhiliang
authors.initials: Z
authors.lastname: Chen
authors.forename: Yilei
authors.initials: Y
authors.lastname: Li
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Wen
mesh_terms: D000470:Alkaloids / Q000008:administration & dosage*; D000818:Animals; D004359:Drug Therapy, Combination; D005260:Female; D019695:Glycyrrhizic Acid / Q000008:administration & dosage*; D008103:Liver Cirrhosis / Q000139:chemically induced / Q000188:drug therapy*; D008297:Male; D008517:Phytotherapy; D011807:Quinolizines / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013853:Thioacetamide; D000093842:Matrines
chemical_list: D000470:Alkaloids; D011807:Quinolizines; D013853:Thioacetamide; D019695:Glycyrrhizic Acid; D000093842:Matrines