title: [Identification of infection agent in diagnostic material from patients with suspicion of enteroviral meningitis].
abstract: Clinical diagnosis of enteroviral infections of the central nervous system are performed by virus isolation in sensitive cell lines and RT-PCR assay. The aim of the study was evaluation these techniques for fast diagnosis meningitis caused by enteroviruses. 69 samples (cerebrospinal fluid, CSF) were collected and analised by RT-PCR reaction. 39 samples were positive (56.5%). 20 positive sample were selected and simultaneously 20 stool samples from the same patients were collected for virus isolation in sensitive cell line. Positive isolation was observed only in one CSF (5.3%) and in 9 stool samples (45%).
authors.affiliation: Zakład Wirusologii, Narodowy Instytut Zdrowia Publicznego - Państwowy Zakład Higieny w Warszawie. awitek@pzh.gov.pl
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Witek
authors.forename: Marta
authors.initials: M
authors.lastname: Brzóstkowska
authors.forename: Anna
authors.initials: A
authors.lastname: Diuwe
authors.forename: Magdalena
authors.initials: M
authors.lastname: Wieczorek
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002555:Cerebrospinal Fluid / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D030015:Enterovirus C, Human / Q000145:classification / Q000302:isolation & purification* / Q000472:pathogenicity*; D004769:Enterovirus Infections / Q000134:cerebrospinal fluid / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology*; D005260:Female; D006801:Humans; D008297:Male; D008587:Meningitis, Viral / Q000134:cerebrospinal fluid / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology*; D008875:Middle Aged; D011044:Poland; D011237:Predictive Value of Tests; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014776:Virus Cultivation
chemical_list: D004279:DNA, Viral