title: Preparation and characterization of recombinant human growth hormone-Zn2+-dextran nanoparticles using aqueous phase-aqueous phase emulsion.
abstract: An effective and simple method was developed to prepare spherical and uniform-sized recombinant human growth hormone (rhGH)-Zn(2+)-dextran nanoparticles (NPs) for a variety of delivery applications of the delicate protein. The results showed that the prepared rhGH-Zn(2+)-dextran particles were spherical, glassy, and uniform in size. rhGH recovered from the NPs maintained its structural integrity and its bioactivity well, as suggested by the Nb(2)-11 cell proliferation assay. Using polyethylene glycol and polysaccharide to mediate Zn(2+)-induced precipitation has proved to be a promising method to prepare spherical protein-loaded NPs with desired sizes and preserved protein stability.
  
  
  BACKGROUND
  This basic science paper discusses a simple yet effective method to prepare spherical and uniform-sized recombinant human growth hormone (rhGH)-Zn(2+)-dextran nanoparticles for a variety of delivery applications.
authors.affiliation: Department of Neurology, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China. yuanweien@126.com
authors.forename: Weien
authors.initials: W
authors.lastname: Yuan
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Hu
authors.forename: Jing
authors.initials: J
authors.lastname: Su
authors.forename: Fei
authors.initials: F
authors.lastname: Wu
authors.forename: Zhenguo
authors.initials: Z
authors.lastname: Liu
authors.forename: Tuo
authors.initials: T
authors.lastname: Jin
mesh_terms: D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D003911:Dextrans / Q000737:chemistry* / Q000494:pharmacology; D004655:Emulsions / Q000737:chemistry; D019382:Human Growth Hormone / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D015032:Zinc / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D003911:Dextrans; D004655:Emulsions; D019382:Human Growth Hormone; D015032:Zinc