title: Mass spectral measurements of the apoHDL in horse (Equus caballus) cerebrospinal fluid.
abstract: As a continuation of our proteogenomic studies of equine apolipoproteins, we have obtained molecular masses for several of the apolipoproteins associated with the HDL in horse cerebrospinal fluid (CSF). Using electrospray-ionization mass spectrometry (ESI-MS), we report on values for apolipoproteins, A-I and A-II, as well as acylated apoA-I. In comparison with our previously published data on equine plasma apolipoproteins, there appears to be a higher percentage of acylated apoA-I in the CSF than in plasma. As was the case in plasma, apoA-II circulates as a homodimer. These studies also revealed a protein with a mass of 34,468Da that we are speculating is the value for horse apoE.
authors.affiliation: The Molecular Biology Institute, University of California, Los Angeles, CA 90095, USA. puppione@chem.ucla.edu
authors.forename: Donald L
authors.initials: DL
authors.lastname: Puppione
authors.forename: Lorenza
authors.initials: L
authors.lastname: Della Donna
authors.forename: Sara
authors.initials: S
authors.lastname: Bassilian
authors.forename: Puneet
authors.initials: P
authors.lastname: Souda
authors.forename: Melinda H
authors.initials: MH
authors.lastname: MacDonald
authors.forename: Julian P
authors.initials: JP
authors.lastname: Whitelegge
mesh_terms: D000818:Animals; D016632:Apolipoprotein A-I / Q000134:cerebrospinal fluid* / Q000737:chemistry; D016633:Apolipoprotein A-II / Q000134:cerebrospinal fluid* / Q000737:chemistry; D006736:Horses / Q000134:cerebrospinal fluid*; D008297:Male; D008970:Molecular Weight; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D016632:Apolipoprotein A-I; D016633:Apolipoprotein A-II