title: Field enhancement sample stacking for analysis of organic acids in traditional Chinese medicine by capillary electrophoresis.
abstract: A technique known as field enhancement sample stacking (FESS) and capillary electrophoresis (CE) separation has been developed to analyze and detect organic acids in the three traditional Chinese medicines (such as Portulaca oleracea L., Crataegus pinnatifida and Aloe vera L.). In FESS, a reverse electrode polarity-stacking mode (REPSM) was applied as on-line preconcentration strategy. Under the optimized condition, the baseline separation of eight organic acids (linolenic acid, lauric acid, p-coumaric acid, ascorbic acid, benzoic acid, caffeic acid, succinic acid and fumaric acid) could be achieved within 20 min. Validation parameters of this method (such as detection limits, linearity and precision) were also evaluated. The detection limits ranged from 0.4 to 60 ng/mL. The results indicated that the proposed method was effective for the separation of mixtures of organic acids. Satisfactory recoveries were also obtained in the analysis of these organic acids in the above traditional Chinese medicine samples.
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, and Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Zhu
authors.forename: Xueqin
authors.initials: X
authors.lastname: Xu
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Huang
authors.forename: Liangjun
authors.initials: L
authors.lastname: Xu
authors.forename: Guonan
authors.initials: G
authors.lastname: Chen
mesh_terms: D001881:Borates / Q000737:chemistry; D001888:Boric Acids / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D009930:Organic Chemicals / Q000032:analysis*; D015203:Reproducibility of Results
chemical_list: D001881:Borates; D001888:Boric Acids; D004365:Drugs, Chinese Herbal; D009930:Organic Chemicals; C010634:sodium borate; C032688:boric acid