title: Immunomodulatory studies of a bioactive fraction from the fruit of Prunus cerasus in BALB/c mice.
abstract: In order to evaluate the role of ethyl acetate fraction (PNRS-EtOAC) obtained from the Prunus cerasus fruit in the modulation of immune responses, detailed studies were carried out using a panel of in vivo assays. Oral administration of PNRS-EtOAC (25-100 mg/kg) stimulated the IgM and IgG titre expressed in the form of hemagglutination antibody (HA) titre. Further, it elicited a dose related increase in the delayed type hypersensitivity reaction (DTH) after 24 and 48 h in BALB/c mice. Besides augmenting the humoral and cell mediated immune response, the concentration of cytokines (IFN-γ, IL-4, and TNF-α) in serum with respect to T cell interactions, i.e. the proliferation of lymphocytes were significantly increased at 50 mg/kg compared with the control. The results in these studies demonstrated the immunostimulatory effect of PNRS-EtOAC in a dose-dependent manner with respect to the macrophage activation possibly expressing the phagocytosis and nitrite production by the enhancement of TNF-α production as a mode of action.
authors.affiliation: Dept. of Biotechnology, Indian Institute of Integrative Medicine (CSIR), Srinagar-190005, India.
authors.forename: Sheikh
authors.initials: S
authors.lastname: Abid
authors.forename: Anamika
authors.initials: A
authors.lastname: Khajuria
authors.forename: Qazi
authors.initials: Q
authors.lastname: Parvaiz
authors.forename: Tabasum
authors.initials: T
authors.lastname: Sidiq
authors.forename: Aruna
authors.initials: A
authors.lastname: Bhatia
authors.forename: Surjeet
authors.initials: S
authors.lastname: Singh
authors.forename: Shabir
authors.initials: S
authors.lastname: Ahmad
authors.forename: M K
authors.initials: MK
authors.lastname: Randhawa
authors.forename: N K
authors.initials: NK
authors.lastname: Satti
authors.forename: Prabhu
authors.initials: P
authors.lastname: Dutt
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D018122:B7-1 Antigen / Q000276:immunology; D051940:B7-2 Antigen / Q000276:immunology; D049109:Cell Proliferation / Q000187:drug effects; D016207:Cytokines / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology; D000431:Ethanol / Q000737:chemistry; D005638:Fruit / Q000737:chemistry*; D006384:Hemagglutination / Q000187:drug effects; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007155:Immunologic Factors / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000276:immunology; D010587:Phagocytosis / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D027861:Prunus; D012756:Sheep; D012997:Solvents / Q000737:chemistry; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology
chemical_list: D018122:B7-1 Antigen; D051940:B7-2 Antigen; C497965:Cd86 protein, mouse; D016207:Cytokines; D007155:Immunologic Factors; D010936:Plant Extracts; D012997:Solvents; D009569:Nitric Oxide; D000431:Ethanol