title: Identification of differential gene expressions in colorectal cancer and polyp by cDNA microarray.
abstract: OBJECTIVE
  To screen the differential expressed genes in colorectal cancer and polyp tissue samples.
  
  
  METHODS
  Tissue specimens containing 16 cases of colorectal adenocarcinoma and colorectal polyp vs normal mucosae were collected and subjected to cDNA microarray and bioinformatical analyses. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to confirm some of the cDNA microarray data.
  
  
  RESULTS
  The experimental data showed that eight genes were differentially expressed, most of which were upregulated in adenomatous polyp lesions. Forty-six genes expressions were altered in colorectal cancers, of which 29 were upregulated and 17 downregulated, as compared to the normal mucosae. In addition, 18 genes were similarly altered in both adenomatous polyps and colorectal cancer. qRT-PCR analyses confirmed the cDNA microarray data for four of those 18 genes: MTA1, PDCD4, TSC1 and PDGFRA.
  
  
  CONCLUSIONS
  These differentially expressed genes likely represent biomarkers for early detection of colorectal cancer and may be potential therapeutic targets after confirmed by further studies.
authors.affiliation: Department of Gastroenterology, Chenggong Hospital, Xiamen University, Xiamen 361003, Fujian Province, China. dyichen@sina.com
authors.forename: Yi-Chen
authors.initials: YC
authors.lastname: Dai
authors.forename: Xiao-San
authors.initials: XS
authors.lastname: Zhu
authors.forename: Qing-Zhen
authors.initials: QZ
authors.lastname: Nan
authors.forename: Zhang-Xin
authors.initials: ZX
authors.lastname: Chen
authors.forename: Jun-Pei
authors.initials: JP
authors.lastname: Xie
authors.forename: Yu-Ka
authors.initials: YK
authors.lastname: Fu
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Lin
authors.forename: Qing-Na
authors.initials: QN
authors.lastname: Lian
authors.forename: Qiao-Fang
authors.initials: QF
authors.lastname: Sang
authors.forename: Xiao-Juan
authors.initials: XJ
authors.lastname: Zhan
mesh_terms: D018256:Adenomatous Polyps / Q000235:genetics / Q000473:pathology; D000328:Adult; D000368:Aged; D015415:Biomarkers; D003111:Colonic Polyps / Q000235:genetics* / Q000473:pathology; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D055815:Young Adult
chemical_list: D015415:Biomarkers