title: Histological and immunohistochemical assessment of liver biopsies in morbidly obese patients.
abstract: To study liver lesions in morbidly obese patients who underwent liver biopsy at the time of bariatric surgery to define histological lesions, especially inflammatory infiltrate, diagnostic categories and the possible influence of gender in this respect.
  
  
  
  110 biopsies (36 males-M- and 76 females -F-) were evaluated and categorised, according to the NAS (NAFLD -non alcoholic fatty liver disease- Activity Score) system and other criteria, as non-NAFLD (15.5%, F predominance), non-alcoholic steatohepatitis (NASH) (16.5%, M predominance), non-alcoholic hepatosteatosis (NAHS) (21%, F predominance) and, the most numerous group, NASH-borderline (NASH-BORD) (47%), with three subgroups, characterised by centrozonal lesions, portal area preferential involvement or affecting both areas. The predominant form of hepatocytesteatosis was mixed with a multivesicular component that was present in most cases with fibroinflammatory portal involvement. Nuclear glycogenosomes were found in greater number of biopsies in patients in the third and sixth decades. Portal inflammation was present in a large number of cases (M predominance); the application of immunohistochemical techniques (myeloperoxidase and CD68 antibodies) to evaluate lobular inflammation revealed "surgical hepatitis" in one third of the cases, and the presence of microgranulomas (CD68+) (M predominance), which were more abundant with increasing lesion severity.
  
  
  
  Portal inflammation and multivesicular hepatocytesteatosis are highly prevalent in morbidly obese patients. This study identifies a new subtype of NASH-BORD characterized by centrizonal and porto-periportal area involvement and the existence of liver biopsies without steatosis. CD68+ microgranulomas constitute an unequivocal marker of lobular inflammation in surgical biopsies and of lesion severity, which is gender-related.
authors.affiliation: Pathology Department, San Cecilio University Hospital, Granada (HUSC) and School of Medicine, University of Granada, Spain. trinidad@ugr.es
authors.forename: T
authors.initials: T
authors.lastname: Caballero
authors.forename: A
authors.initials: A
authors.lastname: Gila
authors.forename: G
authors.initials: G
authors.lastname: Sánchez-Salgado
authors.forename: P
authors.initials: P
authors.lastname: Muñoz de Rueda
authors.forename: J
authors.initials: J
authors.lastname: León
authors.forename: S
authors.initials: S
authors.lastname: Delgado
authors.forename: J A
authors.initials: JA
authors.lastname: Muñoz
authors.forename: M
authors.initials: M
authors.lastname: Caba-Molina
authors.forename: A
authors.initials: A
authors.lastname: Carazo
authors.forename: A
authors.initials: A
authors.lastname: Ruiz-Extremera
authors.forename: J
authors.initials: J
authors.lastname: Salmerón
mesh_terms: D000328:Adult; D000367:Age Factors; D015703:Antigens, CD / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism; D050110:Bariatric Surgery; D015415:Biomarkers / Q000378:metabolism; D001706:Biopsy; D002467:Cell Nucleus / Q000378:metabolism / Q000473:pathology; D005234:Fatty Liver / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D005260:Female; D005355:Fibrosis / Q000473:pathology; D006099:Granuloma / Q000378:metabolism / Q000473:pathology; D022781:Hepatocytes / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008099:Liver / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D065626:Non-alcoholic Fatty Liver Disease; D009767:Obesity, Morbid / Q000175:diagnosis* / Q000378:metabolism / Q000601:surgery; D011168:Portal System / Q000473:pathology; D012737:Sex Factors; D000097382:CD68 Molecule
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D015415:Biomarkers; C067980:CD68 antigen, human; D000097382:CD68 Molecule