title: [Effects of Bushen Huoxue Fang on rat cardiac fibroblast proliferation and collagen production in vitro].
abstract: OBJECTIVE
  To investigate the effects of Bushen Huoxue Fang on the proliferation of rat cardiac fibroblasts and collagen production in the cells.
  
  
  METHODS
  Rat cardiac fibroblasts were isolated and cultured in DMEM containing 10% (group A) or 20% (group B) or no (group C) serum from rats treated with Bushen Huoxue Fang, with cells cultured in DMEM containing 10% FBS as the control (group D). After 72 h of cell culture, the proliferation of the fibroblasts was detected using CCK-8 kit, and collagen mRNA and protein expressions were examined using RT-PCR and Western blotting, respectively.
  
  
  RESULTS
  Compared with that in groups C and D, the cell proliferation decreased significantly in groups A and B, and especially in the latter (P<0.05). RT-PCR demonstrated significant reductions of the mRNAs of type 1 and 3 collagens in groups A and B (P<0.05), and their protein levels were also significantly lowered (P<0.05).
  
  
  CONCLUSIONS
  Bushen Huoxue Fang can effectively inhibit the proliferation of rat cardiac fibroblasts and reduced collagen type 1 and 3 productions in the cells in vitro.
authors.affiliation: Department of Traditional Chinese Medicine, General Hospital of PLA, Beijing, China. xinying719@163.com
authors.forename: Xinying
authors.initials: X
authors.lastname: Ma
authors.forename: Minghui
authors.initials: M
authors.lastname: Yang
authors.forename: Jinsong
authors.initials: J
authors.lastname: Chen
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Congze
authors.initials: C
authors.lastname: Pan
authors.forename: Jinzhuo
authors.initials: J
authors.lastname: Fan
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D024042:Collagen Type I / Q000096:biosynthesis; D024061:Collagen Type III / Q000096:biosynthesis; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D005355:Fibrosis / Q000517:prevention & control; D009206:Myocardium / Q000166:cytology* / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D024042:Collagen Type I; D024061:Collagen Type III; D004365:Drugs, Chinese Herbal; C527851:bushen huoxue