title: Klp10A, a microtubule-depolymerizing kinesin-13, cooperates with CP110 to control Drosophila centriole length.
abstract: Klp10A is a kinesin-13 of Drosophila melanogaster that depolymerizes cytoplasmic microtubules. In interphase, it promotes microtubule catastrophe; in mitosis, it contributes to anaphase chromosome movement by enabling tubulin flux. Here we show that Klp10A also acts as a microtubule depolymerase on centriolar microtubules to regulate centriole length. Thus, in both cultured cell lines and the testes, absence of Klp10A leads to longer centrioles that show incomplete 9-fold symmetry at their ends. These structures and associated pericentriolar material undergo fragmentation. We also show that in contrast to mammalian cells where depletion of CP110 leads to centriole elongation, in Drosophila cells it results in centriole length diminution that is overcome by codepletion of Klp10A to give longer centrioles than usual. We discuss how loss of centriole capping by CP110 might have different consequences for centriole length in mammalian and insect cells and also relate these findings to the functional interactions between mammalian CP110 and another kinesin-13, Kif24, that in mammalian cells regulates cilium formation.
authors.affiliation: Cancer Research UK Cell Cycle Genetics Research Group, Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Delgehyr
authors.forename: Hélène
authors.initials: H
authors.lastname: Rangone
authors.forename: Jingyan
authors.initials: J
authors.lastname: Fu
authors.forename: Guojie
authors.initials: G
authors.lastname: Mao
authors.forename: Brian
authors.initials: B
authors.lastname: Tom
authors.forename: Maria Giovanna
authors.initials: MG
authors.lastname: Riparbelli
authors.forename: Giuliano
authors.initials: G
authors.lastname: Callaini
authors.forename: David M
authors.initials: DM
authors.lastname: Glover
mesh_terms: D000818:Animals; D001483:Base Sequence; D018797:Cell Cycle Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D002502:Centrioles / Q000378:metabolism* / Q000648:ultrastructure*; D017931:DNA Primers / Q000235:genetics; D029721:Drosophila Proteins / Q000037:antagonists & inhibitors / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure*; D017344:Genes, Insect; D006801:Humans; D016547:Kinesins / Q000037:antagonists & inhibitors / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D008297:Male; D046529:Microscopy, Electron, Transmission; D008869:Microtubule-Associated Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D010750:Phosphoproteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013090:Spermatocytes / Q000378:metabolism / Q000648:ultrastructure; D013737:Testis / Q000378:metabolism / Q000648:ultrastructure
chemical_list: C467559:CCP110 protein, human; D018797:Cell Cycle Proteins; D017931:DNA Primers; D029721:Drosophila Proteins; D008869:Microtubule-Associated Proteins; D010750:Phosphoproteins; D011993:Recombinant Fusion Proteins; C481339:KLP10A protein, Drosophila; D016547:Kinesins