title: Platelet-rich plasma loaded hydrogel scaffold enhances chondrogenic differentiation and maturation with up-regulation of CB1 and CB2.
abstract: Three-dimensional scaffolds like hydrogels can be used for cell and drug delivery and have become a major research focus in tissue engineering. Presently, we investigated the regenerative potency of platelet-rich plasma (PRP) combined with a chondrocyte/hydrogel composite scaffold in the repair of articular cartilage defects using a rabbit model. Primary isolated joint chondrocytes from the trachlear groove of rabbit were cultured in hydrogels as follows; hydrogel (2900 Pa or 5900 Pa)+chondrocytes and hydrogel+chondrocytes+PRP for in vitro analysis and in vivo implantation. The 5900 Pa hydrogel markedly increased cellular viability and development in a time-dependent manner. Furthermore, the hydrogels attenuated the expression of SOX-9, aggrecan, and type II collagen. PRP-containing hydrogels produced an immediate increase in mRNA levels of cannabinoid receptor (CB)1 and CB2, compared with control and PRP-free hydrogels. Osteochondral defects were enhanced recovery with formation of cartilage and perichondrium in the 5900 Pa hydrogel+chondrocytes+PRP. Hydrogel may provide a suitable environment for proliferation and maturation of joint chondrocytes in relation to the gelation density and bioactive sources like PRP resulting in improvement for cartilage regeneration.
authors.affiliation: Department of Veterinary Clinical Pathology, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Hye-Rim
authors.initials: HR
authors.lastname: Lee
authors.forename: Kyung Min
authors.initials: KM
authors.lastname: Park
authors.forename: Yoon Ki
authors.initials: YK
authors.lastname: Joung
authors.forename: Ki Dong
authors.initials: KD
authors.lastname: Park
authors.forename: Sun Hee
authors.initials: SH
authors.lastname: Do
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002358:Cartilage, Articular / Q000166:cytology / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism; D020219:Chondrogenesis; D020100:Hydrogels / Q000737:chemistry*; D008297:Male; D053657:Platelet-Rich Plasma / Q000737:chemistry*; D011817:Rabbits; D043884:Receptor, Cannabinoid, CB1 / Q000096:biosynthesis*; D043885:Receptor, Cannabinoid, CB2 / Q000096:biosynthesis*; D012038:Regeneration; D020133:Reverse Transcriptase Polymerase Chain Reaction; D023822:Tissue Engineering / Q000379:methods*; D015854:Up-Regulation
chemical_list: D001672:Biocompatible Materials; D020100:Hydrogels; D043884:Receptor, Cannabinoid, CB1; D043885:Receptor, Cannabinoid, CB2