title: Human mast cells (HMC-1 5C6) enhance interleukin-6 production by quiescent and lipopolysaccharide-stimulated human coronary artery endothelial cells.
abstract: We examined the effect of intact human mast cells (HMC-1 5C6) and their selected mediators on interleukin-6 (IL-6) production and bone morphogenetic protein-2 (BMP-2) expression in human coronary artery endothelial cells (HCAEC) in the presence and absence of lipopolysaccharide (LPS). Scanning electron microscopy showed that HMC-1 5C6 cells adhere to HCAEC in cocultures. Addition of HMC-1 5C6 cells markedly enhanced the IL-6 production by quiescent and LPS-activated HCAEC even at the maximal concentration of LPS. Furthermore, mast cell-derived histamine and proteases accounted for the direct and synergistic effect of mast cells on IL-6 production that was completely blocked by the combination of histamine receptor-1 antagonist and protease inhibitors. Another novel finding is that histamine was able to induce BMP-2 expression in HCAEC. Collectively, our results suggest that endotoxin and mast cell products synergistically amplify vascular inflammation and that histamine participates in the early events of vascular calcification.
authors.affiliation: Division of Allergy, Clinical Immunology, and Rheumatology, Department of Internal Medicine, University of Kansas Medical Center, Kansas City, 66160, USA.
authors.forename: Damandeep S
authors.initials: DS
authors.lastname: Walia
authors.forename: Mukut
authors.initials: M
authors.lastname: Sharma
authors.forename: Vineesh V
authors.initials: VV
authors.lastname: Raveendran
authors.forename: Jianping
authors.initials: J
authors.lastname: Zhou
authors.forename: Ram
authors.initials: R
authors.lastname: Sharma
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Stechschulte
authors.forename: Kottarappat N
authors.initials: KN
authors.lastname: Dileepan
mesh_terms: D015153:Blotting, Western; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics / Q000378:metabolism; D002450:Cell Communication / Q000235:genetics / Q000502:physiology; D002478:Cells, Cultured; D003331:Coronary Vessels / Q000166:cytology*; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects*; D006801:Humans; D015850:Interleukin-6; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008407:Mast Cells / Q000166:cytology* / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D015850:Interleukin-6; D008070:Lipopolysaccharides