title: [Role of Slit-Robo signaling in the proliferation of human mucoepidermoid carcinoma Mc3 cells].
abstract: OBJECTIVE
  To study the role of Slit-Robo signaling in the proliferation of human mucoepidermoid carcinoma Mc3 cells.
  
  
  METHODS
  We measured the expressions of Slit2 and Robo1 proteins in human mucoepidermoid carcinoma Mc3 cells using immunohistochemistry and flow cytometry. To test the role of Slit-Robo signaling in the proliferation of the cells, we treated the cells with the monoclonal antibody R5 against Robo1 receptor extracellular domain and observed the changes in the cell proliferation by cell counting and colonogenic assay; the expression of proliferating cell nuclear antigen (PCNA) in the cells following the treatment was measured with flow cytometry.
  
  
  RESULTS
  Treatment of Mc3 cells with the monoclonal antibody R5 caused significantly suppressed cell growth and proliferation and obviously lowered the expression of PCNA.
  
  
  CONCLUSIONS
  Slit-Robo signaling can suppress the proliferation of Mc3 cells possibly by up-regulating the expression of PCNA.
authors.affiliation: North-west University for Nationalities, Lanzhou, China. pizza3625@yahoo.com.cn
authors.forename: Bing
authors.initials: B
authors.lastname: Han
authors.forename: Lijing
authors.initials: L
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Cuiling
authors.initials: C
authors.lastname: Qi
mesh_terms: D018277:Carcinoma, Mucoepidermoid / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D011971:Receptors, Immunologic / Q000378:metabolism*; D012468:Salivary Gland Neoplasms / Q000473:pathology; D015398:Signal Transduction; D000097905:Roundabout Proteins; D000098552:Slit Homolog 2 Protein
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; D018809:Proliferating Cell Nuclear Antigen; D011971:Receptors, Immunologic; D000097905:Roundabout Proteins; D000098552:Slit Homolog 2 Protein