title: Antimicrobial activities against biofilm formed by Proteus mirabilis isolates from wound and urinary tract infections.
abstract: BACKGROUND
  Bacterial species are capable of living as biofilm and/or planktonic forms. There is increasing evidence for the role of bacterial biofilm in various wound and urinary tract infections (UTIs). The aim of the present study was to evaluate the ability of the bacteria, isolated from urinary tract infections (UTIs) and wound infections, to form biofilm and correlate the role of biofilm with their antimicrobial resistance.
  
  
  METHODS
  All the isolated bacteria were screened for their ability to form biofilm using the microtitre plate method.
  
  
  RESULTS
  Wound isolates of Staphylococcus aureus and Enterobacter sp. had more biofilm forming capacity than the UTI isolates. Proteus mirabilis isolates were among the strongest biofilm forming bacteria and were chosen for antimicrobial study. In sub-MIC concentrations of antimicrobial agents used, ciprofloxacin was found to be the most effective in decreasing biofilm formation. On the other hand, ceftriaxone and ciprofloxacin were effective in partial removal of preformed biofilm biomass.
  
  
  CONCLUSIONS
  Ciprofloxacin was more effective in killing bacterial cells especially at high antimicrobial concentrations that could be reached in urine levels and can be used in impregenating catheters.
authors.affiliation: Department of Microbiology, Faculty of Pharmacy, University for Modern Sciences and Arts, Giza, Egypt. wasfi.reham@gmail.com
authors.forename: R
authors.initials: R
authors.lastname: Wasfi
authors.forename: O A
authors.initials: OA
authors.lastname: Abd El-Rahman
authors.forename: L E
authors.initials: LE
authors.lastname: Mansour
authors.forename: A S
authors.initials: AS
authors.lastname: Hanora
authors.forename: A M
authors.initials: AM
authors.lastname: Hashem
authors.forename: M S
authors.initials: MS
authors.lastname: Ashour
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D024881:Drug Resistance, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D011512:Proteus Infections / Q000382:microbiology*; D011513:Proteus mirabilis / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification; D014552:Urinary Tract Infections / Q000382:microbiology*; D014946:Wound Infection / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents