title: The use of chitosan based hydrogel for enhancing the therapeutic benefits of adipose-derived MSCs for acute kidney injury.
abstract: Transplantation of mesenchymal stem cells (MSCs) has been reported a great therapeutic potential for acute kidney injury (AKI). However, the therapeutic benefits are limited due to the low retention and survival of transplanted cells within target sites. In this study, thermosensitive chitosan chloride (CSCl) hydrogel was explored as injectable scaffold for adipose-derived MSCs (ADMSCs) delivery into ischemia/reperfusion (I/R) induced acute kidney injury (AKI). Thermosensitive CSCl hydrogels with/without ADMSCs were injected into the I/R site of rat AKI models. Dihydroethidium staining was used to detect the number of ROS in vivo. In order to track ADMSCs in vivo, ADMSCs were transfected with firefly luciferase and monomeric red fluorescent protein reporter genes (fluc-mrfp). The retention and survival of ADMSC were assessed using bioluminescence imaging, differentiation behaviors of ADMSCs were investigated using immunofluorescent and immunohistochemical staining. Proliferation and apoptosis of host renal cell in vivo were characterized by PCNA and TUNEL staining. Results suggested that CSCl hydrogels could improve the retention and survival of grafted ADMSCs, moreover, CSCl hydrogels could enhance the proliferation activity and reduce apoptosis of host renal cells. At 4 weeks, significant improvement of the renal function, microvessel density and tubular cell proliferation were observed in CSCl hydrogels with ADMSCs groups. Therefore, the application of thermosensitive CSCl hydrogel as scaffold for ADMSCs delivery into renal region could resolve the main obstacle of cell transplantation for acute kidney injury (AKI). Therefore, CSCl hydrogel is a potential cell carrier for treatment of AKI.
authors.affiliation: Department of Advanced Interdisciplinary Studies, Institute of Basic Medical Sciences and Tissue Engineering Research Center, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Jiasheng
authors.initials: J
authors.lastname: Gao
authors.forename: Rongfu
authors.initials: R
authors.lastname: Liu
authors.forename: Jie
authors.initials: J
authors.lastname: Wu
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Liu
authors.forename: Junjie
authors.initials: J
authors.lastname: Li
authors.forename: Jin
authors.initials: J
authors.lastname: Zhou
authors.forename: Tong
authors.initials: T
authors.lastname: Hao
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Zhiyan
authors.initials: Z
authors.lastname: Du
authors.forename: Cuimi
authors.initials: C
authors.lastname: Duan
authors.forename: Changyong
authors.initials: C
authors.lastname: Wang
mesh_terms: D058186:Acute Kidney Injury / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D001672:Biocompatible Materials; D001806:Blood Urea Nitrogen; D002454:Cell Differentiation; D002470:Cell Survival; D048271:Chitosan; D003404:Creatinine / Q000097:blood; D017930:Genes, Reporter; D020100:Hydrogels; D008297:Male; D008422:Materials Testing; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D018919:Neovascularization, Physiologic; D051381:Rats; D017207:Rats, Sprague-Dawley; D054457:Tissue Scaffolds / Q000737:chemistry; D014162:Transfection
chemical_list: D001672:Biocompatible Materials; D020100:Hydrogels; D048271:Chitosan; D003404:Creatinine