title: Effect of copper on the expression of IGF-1 from chondrocytes in newborn piglets in vitro.
abstract: The experiment was performed to evaluate the influence of copper supplementation on insulin-like growth factor-1 (IGF-1) mRNA expression in chondrocytes of newborn pigs. Chondrocytes were isolated and cultured in media containing 15% fetal calf serum supplemented with 0, 15.6, 31.2, and 62.5 μmol/L copper in 90-mm culture plate. After 0, 12, 24, and 48 h, total RNA was isolated from chondrocytes. Then, IGF-1 mRNA expression was determined by semiquantitative RT-PCR. The results showed that the expression of IGF-1 mRNA, adjusted for β-actin expression, was increased in the culture media added to 15.6, 31.2, and 62.5 μmol/L copper, respectively. In the present experiment, the optimal copper concentration and optimal culture time for the expression of IGF-1 mRNA were 31.2 μmol/L and 48 h, respectively.
authors.affiliation: College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Wang
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zhu
authors.forename: Guanghong
authors.initials: G
authors.lastname: Xie
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zhaoxi
authors.initials: Z
authors.lastname: Liu
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.forename: Chenxu
authors.initials: C
authors.lastname: Zhao
authors.forename: Wentao
authors.initials: W
authors.lastname: Yang
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Shi
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wang
authors.forename: Guowen
authors.initials: G
authors.lastname: Liu
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D049109:Cell Proliferation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects* / Q000378:metabolism; D003300:Copper / Q000378:metabolism / Q000494:pharmacology*; D019327:Copper Sulfate / Q000378:metabolism / Q000494:pharmacology; D003470:Culture Media / Q000378:metabolism; D004353:Drug Evaluation, Preclinical; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D013997:Time Factors
chemical_list: D000199:Actins; D003470:Culture Media; D012333:RNA, Messenger; D007334:Insulin-Like Growth Factor I; D003300:Copper; D019327:Copper Sulfate