title: Morphometric and molecular characterization of the series Guyanensis (Diptera, Psychodidae, Psychodopygus) from the Ecuadorian Amazon Basin with description of a new species.
abstract: Several populations of the series Guyanensis (Diptera, Psychodidae, Psychodopygus) were collected from the Yasuní National Park in the Ecuadorian Amazon region. The specimens comprised the species Psychodopygus geniculatus, Psychodopygus luisleoni and Psychodopygus corossoniensis. Within Ps. geniculatus, we observed two populations, one with a narrow paramere and relatively short genital filaments and the other characterized by a wider coxite and longer genital filaments. A multiple approach combining morphology, morphometry and DNA sequencing of the ribosomal internal transcribed spacer 2 (ITS2) and the mitochondrial cytochrome b gene was carried out. Morphological, morphometric and molecular data strongly suggested the presence of two populations within Ps. geniculatus. The lack of intermediate forms within these populations supported the proposal of two sympatric species. This report describes Psychodopygus francoisleponti n. sp.
authors.affiliation: Université de Reims Champagne-Ardenne, ANSES, EA4688, USC Transmission vectorielle et épidémiosurveillance de maladies parasitaires (VECPAR), France.
authors.forename: S
authors.initials: S
authors.lastname: Zapata
authors.forename: R
authors.initials: R
authors.lastname: León
authors.forename: F
authors.initials: F
authors.lastname: Sauvage
authors.forename: D
authors.initials: D
authors.lastname: Augot
authors.forename: G
authors.initials: G
authors.lastname: Trueba
authors.forename: C
authors.initials: C
authors.lastname: Cruaud
authors.forename: A
authors.initials: A
authors.lastname: Couloux
authors.forename: R
authors.initials: R
authors.lastname: Terán
authors.forename: J
authors.initials: J
authors.lastname: Depaquit
mesh_terms: D000818:Animals; D057506:Arthropod Antennae / Q000033:anatomy & histology; D001483:Base Sequence; D016000:Cluster Analysis; D045303:Cytochromes b / Q000235:genetics; D021901:DNA, Intergenic / Q000235:genetics; D016002:Discriminant Analysis; D004484:Ecuador; D005260:Female; D005837:Genitalia, Male / Q000033:anatomy & histology; D006257:Head / Q000033:anatomy & histology; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D025341:Principal Component Analysis; D011576:Psychodidae / Q000033:anatomy & histology / Q000145:classification* / Q000235:genetics; D016415:Sequence Alignment; D061350:Sympatry; D014921:Wings, Animal / Q000033:anatomy & histology
chemical_list: D021901:DNA, Intergenic; D045303:Cytochromes b