title: Anti-tick monoclonal antibody applied by artificial capillary feeding in Rhipicephalus (Boophilus) microplus females.
abstract: The tick Rhipicephalus microplus is an ectoparasite harmful to livestock, a vector of disease agents that affects meat and milk production. However, resistance to acaricides reflects the need for alternative tick control methods, among which vaccines have gained increasing relevance. In this scenario, monoclonal antibodies can be used to identify and characterize antigens that can be used as vaccine immunogens. Capillary tube artificial feeding of partially engorged R. microplus females with monoclonal antibodies against proteins from the gut of tick were used to test the effects of immunoglobulins in the physiology of the parasite. The results of artificial feeding showed that female ticks over 25mg and under 60 mg in weight performed better in the artificial feeding process, with a 94-168% weight increase after 24h of feeding. Results showed that artificial feeding of ticks proved to be a viable technique to study the effects of antibodies or drugs in the physiology of the parasite. One monoclonal antibody (BrBm2) induced decreased oviposition. Moreover, the antigen recognized by BrBm2 was identified as a 27-kDa protein and immunolabeled on digestive vesicles membranes of digestive cells of partially and fully engorged females.
authors.affiliation: Centro de Biotecnologia do Estado do Rio Grande do Sul, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9500, C.P. 15005, 91501-970 Porto Alegre, RS, Brazil.
authors.forename: Andressa Varella
authors.initials: AV
authors.lastname: Gonsioroski
authors.forename: Isis Abel
authors.initials: IA
authors.lastname: Bezerra
authors.forename: Kiyoko Uemura
authors.initials: KU
authors.lastname: Utiumi
authors.forename: David
authors.initials: D
authors.lastname: Driemeier
authors.forename: Sandra Estrazulas
authors.initials: SE
authors.lastname: Farias
authors.forename: Itabajara
authors.initials: I
authors.lastname: da Silva Vaz
authors.forename: Aoi
authors.initials: A
authors.lastname: Masuda
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage*; D000941:Antigens / Q000032:analysis / Q000276:immunology; D015153:Blotting, Western; D060166:Capillary Tubing; D002417:Cattle; D005260:Female; D006825:Hybridomas; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D010058:Oviposition / Q000276:immunology; D048494:Rhipicephalus / Q000276:immunology*; D013983:Tick Control / Q000379:methods; D014612:Vaccines
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens; D014612:Vaccines