title: Haploidization in Saccharomyces cerevisiae induced by a deficiency in homologous recombination.
abstract: Diploid Saccharomyes cerevisae strains lacking the RAD52 gene required for homologous recombination have a very high rate of chromosome loss. Two of four isolates subcultured ∼20 times (∼500 cell divisions) became haploid. These strains were capable of mating with wild-type haploids to produce diploid progeny capable of undergoing meiosis to produce four viable spores.
authors.affiliation: Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina 27710, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Song
authors.forename: Thomas D
authors.initials: TD
authors.lastname: Petes
mesh_terms: D015825:Chromosomes, Fungal / Q000235:genetics; D017353:Gene Deletion; D006238:Haploidy; D059765:Homologous Recombination / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D051721:Rad52 DNA Repair and Recombination Protein / Q000172:deficiency / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics
chemical_list: C497197:RAD52 protein, S cerevisiae; D051721:Rad52 DNA Repair and Recombination Protein; D029701:Saccharomyces cerevisiae Proteins