title: Ultrarapid detection of blaKPC₁/₂-₁₂ from perirectal and nasal swabs by use of real-time PCR.
abstract: The novel real-time PCR assay developed as described here was able to detect bla(KPC1/2-12) (bla(KPC-1/2) to bla(KPC-12)) from easily available clinical specimens in less than 2 h. The genotypic assay was highly sensitive (100%) and specific (98%). In some cases, it was able to detect bla(KPC) 48 h before positive detection by standard phenotypic assay on patients who were monitored daily. The high sensitivity and rapidity of the molecular method make it the method of choice for KPC surveillance and, thus, containment purposes.
authors.affiliation: Department of Molecular Medicine, Padua, Italy. sara.richter@unipd.it
authors.forename: Sara N
authors.initials: SN
authors.lastname: Richter
authors.forename: Ilaria
authors.initials: I
authors.lastname: Frasson
authors.forename: Maria Angela
authors.initials: MA
authors.lastname: Biasolo
authors.forename: Andrea
authors.initials: A
authors.lastname: Bartolini
authors.forename: Antonietta
authors.initials: A
authors.lastname: Cavallaro
authors.forename: Giorgio
authors.initials: G
authors.lastname: Palù
mesh_terms: D001419:Bacteria / Q000201:enzymology* / Q000235:genetics*; D001431:Bacteriological Techniques / Q000379:methods*; D006801:Humans; D010502:Perineum / Q000382:microbiology*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013997:Time Factors; D001618:beta-Lactamases / Q000032:analysis* / Q000235:genetics*
chemical_list: D001618:beta-Lactamases