title: Pleiotrophin expression during odontogenesis.
abstract: Pleiotrophin (PTN) is an extracellular matrix-associated growth factor and chemokine expressed in mesodermal and ectodermal cells. It plays an important role in osteoblast recruitment and differentiation. There is limited information currently available about PTN expression during odontoblast differentiation and tooth formation, and thus the authors aimed to establish the spatiotemporal expression pattern of PTN during mouse odontogenesis. Immortalized mouse dental pulp (MD10-D3, MD10-A11) and odontoblast-like (M06-G3) and ameloblast-like (EOE-3M) cell lines were grown and samples prepared for immunocytochemistry, Western blot, and conventional and quantitative PCR analysis. Effects of BMP2, BMP4, and BMP7 treatment on PTN expression in odontoblast-like M06-G3 cells were tested by quantitative PCR. Finally, immunohistochemistry of sectioned mice mandibles and maxillaries at developmental stages E16, E18, P1, P6, P10, and P28 was performed. The experiments showed that PTN, at both the mRNA and protein level, was expressed in all tested epithelial and mesenchymal dental cell lines and that the level of PTN mRNA was influenced differentially by the bone morphogenetic proteins. The authors observed initial expression of PTN in the inner enamel epithelium with prolonged expression in the ameloblasts and odontoblasts throughout their stages of maturation and strong expression in the terminally differentiated and enamel matrix-secreting ameloblasts and odontoblasts of the adult mouse incisors and molars.
authors.affiliation: Institute of Oral Health Research, School of Dentistry, University of Alabama at Birmingham, Birmingham, Alabama 35294-0007, USA. herlands@uab.edu
authors.forename: Heidi
authors.initials: H
authors.lastname: Erlandsen
authors.forename: Jennifer E
authors.initials: JE
authors.lastname: Ames
authors.forename: Amena
authors.initials: A
authors.lastname: Tamkenath
authors.forename: Olga
authors.initials: O
authors.lastname: Mamaeva
authors.forename: Katherine
authors.initials: K
authors.lastname: Stidham
authors.forename: Mary E
authors.initials: ME
authors.lastname: Wilson
authors.forename: Pablo
authors.initials: P
authors.lastname: Perez-Pinera
authors.forename: Thomas F
authors.initials: TF
authors.lastname: Deuel
authors.forename: Mary
authors.initials: M
authors.lastname: Macdougall
mesh_terms: D000565:Ameloblasts / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology; D055415:Bone Morphogenetic Protein 4 / Q000494:pharmacology; D055419:Bone Morphogenetic Protein 7 / Q000494:pharmacology; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D003782:Dental Pulp / Q000378:metabolism; D007150:Immunohistochemistry; D007180:Incisor / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D008648:Mesoderm / Q000196:embryology / Q000254:growth & development; D051379:Mice; D008810:Mice, Inbred C57BL; D008963:Molar / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D009804:Odontoblasts / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014070:Tooth / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*
chemical_list: D055396:Bone Morphogenetic Protein 2; D055415:Bone Morphogenetic Protein 4; D055419:Bone Morphogenetic Protein 7; D002352:Carrier Proteins; D016207:Cytokines; D012333:RNA, Messenger; C066845:pleiotrophin