title: Functional characterization of a Cassette-specific promoter in the class 1 integron-associated qnrVC1 gene.
abstract: Integrons are natural expression vectors due to the presence of an intrinsic promoter (P(c)). Although rare, gene cassettes can harbor their own promoter. This study determined the functionality of an internal promoter in the qnrVC1 cassette whose presence was suggested by a level of transcription similar to that of the preceding cassette (aadA2) and confirmed by in silico analysis. Its functionality was determined by 5' rapid amplification of cDNA ends (RACE) and cloning into promoter-probe vectors. P(qnrVC) was found in the qnrVC cassette family, stressing its role in contributing to resistance manifestation.
authors.affiliation: Laboratório de Genética Molecular de Microrganismos, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil.
authors.forename: Érica Lourenço
authors.initials: ÉL
authors.lastname: da Fonseca
authors.forename: Ana Carolina Paulo
authors.initials: AC
authors.lastname: Vicente
mesh_terms: D018076:DNA, Complementary / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D032023:Integrons / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*
chemical_list: D018076:DNA, Complementary