title: [Discrimination of alleles in HLA-C*07:01:01G and HLA-C*07:02:01G groups through detection sequences in exons 1 to 7 of HLA-C locus by using polymerase chain reaction sequence-based typing].
abstract: This study was aimed to discriminate the alleles in the HLA-C*07:01:01G and HLA-C*07:02:01G groups and analyze their associations with HLA-B locus. Samples previously typed as HLA-C*07:01:01G and HLA-C*07:02:01G were collected. The nucleotide sequences in exons 1 to 7 of the HLA-C locus were sequenced by polymerase chain reaction sequence-based typing (PCR-SBT) and HLA-B genotyping was also preformed by PCR-SBT in these samples. The results showed that 4 samples (30.8%) were confirmed as HLA-C*07:01:01 and 9 samples (69.2%) were HLA-C*07:06 among 13 samples previously typed as HLA-C*07:01:01G. Linkage disequilibrium (LD) analysis showed that HLA-C*07:06 allele was strongly related with HLA-B*44:03. All samples were typed as C*07:02:01 among 102 individuals previously typed as C*07:02:01G. LD analysis showed that C*07:02:01 was strongly related with HLA-B*51:01, B*46:01, B*39:01, B*40:01, B*38:02, B*15:02 alleles. It is concluded that HLA-C*07:01:01 and HLA-C*07:06 alleles are confirmed in the HLA-C*07:01:01G group and HLA-C*07:02:01 is a preferred allele in the HLA-C*07:02:01G.
authors.affiliation: Institute of Transfusion Medicine, Blood Center of Zhejiang Province, Hangzhou, Zhejiang Province, China. lhj@zjb.org.cn
authors.forename: Hang-Jun
authors.initials: HJ
authors.lastname: Lü
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Jun-Jun
authors.initials: JJ
authors.lastname: He
authors.forename: Yan-Min
authors.initials: YM
authors.lastname: He
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Zhe-Dong
authors.initials: ZD
authors.lastname: Hang
authors.forename: Nan-Yin
authors.initials: NY
authors.lastname: Chen
authors.forename: Fa-Ming
authors.initials: FM
authors.lastname: Zhu
authors.forename: Li-Xing
authors.initials: LX
authors.lastname: Yan
mesh_terms: D000483:Alleles; D001483:Base Sequence; D005091:Exons; D015235:HLA-B Antigens / Q000235:genetics; D015236:HLA-C Antigens / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA
chemical_list: D015235:HLA-B Antigens; D015236:HLA-C Antigens