title: [Determination of tortoside A in Ilicis pubescentis by RP-HPLC].
abstract: OBJECTIVE
  To establish a RP-HPLC method for the determination of tortoside A in Ilex pubences.
  
  
  METHODS
  Kromasil-C18 (4.6 mm x 250 mm, 5 microm) column was used in HPLC with mobile phase acetonitrite-0.1% H3PO4 (17:83), the column temperature was 30 degrees C, the flow rate was 1 mL x min(-1), the detection wavelength was set at 210 nm, and inject volume was 10 microL RESULT: Tortoside A was well separated under the established conditions, the liner range of tortoside A was 26.05-521.00 microg (r = 0.999 9, n = 6), and the average recovery was 98.42%.
  
  
  CONCLUSIONS
  It was the first time to establish the RP-HPLC method with accuracy, good reproducibility for determining the content of tortoside A in I. Pubescentis.
authors.affiliation: Binzhou Vocational College, Binzhou 256603, China. bzlishuli@163.com
authors.forename: Shuli
authors.initials: S
authors.lastname: Li
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Pengfei
authors.initials: P
authors.lastname: Tu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006027:Glycosides / Q000032:analysis*; D030017:Ilex / Q000737:chemistry*
chemical_list: D006027:Glycosides; C570823:tortoside A