title: Nanopatterned cadmium selenide Langmuir-Blodgett platform for leukemia detection.
abstract: We present results of the studies relating to preparation of Langmuir-Blodgett (LB) monolayers of tri-n-octylphosphine oxide-capped cadmium selenide quantum dots (QCdSe) onto indium-tin oxide (ITO) coated glass substrate. The monolayer behavior has been studied at the air-water interface under various subphase conditions. This nanopatterned platform has been explored to fabricate an electrochemical DNA biosensor for detection of chronic myelogenous leukemia (CML) by covalently immobilizing the thiol-terminated oligonucleotide probe sequence via a displacement reaction. The results of electrochemical response studies reveal that this biosensor can detect target DNA in the range of 10(-6) to 10(-14) M within 120 s, has a shelf life of 2 months, and can be used  about 8 times. Further, this nucleic acid sensor has been found to distinguish the CML-positive and the control negative clinical patient samples.
authors.affiliation: Department of Science & Technology Centre on Biomolecular Electronics, Biomedical Instrumentation Section, Materials Physics & Engineering Division, National Physical Laboratory (Council of Scientific & Industrial Research), Dr K. S. Krishnan Marg, New Delhi 110012, India.
authors.forename: Aditya
authors.initials: A
authors.lastname: Sharma
authors.forename: Chandra M
authors.initials: CM
authors.lastname: Pandey
authors.forename: Zimple
authors.initials: Z
authors.lastname: Matharu
authors.forename: Udit
authors.initials: U
authors.lastname: Soni
authors.forename: Sameer
authors.initials: S
authors.lastname: Sapra
authors.forename: Gajjala
authors.initials: G
authors.lastname: Sumana
authors.forename: Manoj K
authors.initials: MK
authors.lastname: Pandey
authors.forename: Tathagat
authors.initials: T
authors.lastname: Chatterjee
authors.forename: Bansi D
authors.initials: BD
authors.lastname: Malhotra
mesh_terms: D001483:Base Sequence; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D019187:Cadmium Compounds / Q000737:chemistry*; D015342:DNA Probes / Q000737:chemistry / Q000235:genetics; D004563:Electrochemistry; D004566:Electrodes; D017743:Equipment Reuse; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000175:diagnosis*; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D009693:Nucleic Acid Hybridization; D009943:Organophosphorus Compounds / Q000737:chemistry; D045663:Quantum Dots; D018036:Selenium Compounds / Q000737:chemistry*; D013499:Surface Properties; D017971:Tin Compounds / Q000737:chemistry
chemical_list: D019187:Cadmium Compounds; D015342:DNA Probes; D009943:Organophosphorus Compounds; D018036:Selenium Compounds; D017971:Tin Compounds; C109984:indium tin oxide; C044965:trioctyl phosphine oxide; C058667:cadmium selenide