title: Chromogenic platform based on recombinant Drosophila melanogaster acetylcholinesterase for visible unidirectional assay of organophosphate and carbamate insecticide residues.
abstract: In this study we propose a chromogenic platform for rapid analysis of organophosphate (OP) and carbamate (CM) insecticide residues, based on recombinant Drosophila melanogaster acetylcholinesterase (R-DmAChE) as enzyme and indoxyl acetate as substrate. The visible chromogenic strip had the advantages identical to those of commonly used lateral flow assays (LFAs) with utmost simplicity in sample loading and result observation. After optimization, depending on the color intensity (CI) values, the well-established assay has the capabilities of both qualitative measurement via naked eyes and quantitative analysis by colorimetric reader with the desirable IC(50) values against the tested six insecticides (0.06 μg mL(-1) of carbofuran, 0.28 μg mL(-1) of methomyl, 0.03 μg mL(-1) of dichlorvos, 31.6 μg mL(-1) of methamidophos, 2.0 μg mL(-1) of monocrotophos, 6.3 μg mL(-1) of omethoate). Acceptable matrix effects and satisfactory detection performance were confirmed by in-parallel LC-MS/MS analysis in different vegetable varieties at various spiked levels of 10(-3) to 10(1) μg g(-1). Overall, the testified suitability and applicability of this novel platform meet the requirements for practical use in food safety management and environmental monitoring, especially in the developing world.
authors.affiliation: Institute for Agri-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, 1018 Jinqi Road, Shanghai 201403, PR China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Han
authors.forename: Chensen
authors.initials: C
authors.lastname: Chi
authors.forename: Bing
authors.initials: B
authors.lastname: Bai
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
authors.forename: Qinxiong
authors.initials: Q
authors.lastname: Rao
authors.forename: Shaojie
authors.initials: S
authors.lastname: Peng
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Zhao
authors.forename: Dabing
authors.initials: D
authors.lastname: Zhang
authors.forename: Aibo
authors.initials: A
authors.lastname: Wu
mesh_terms: D000110:Acetylcholinesterase / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002219:Carbamates / Q000032:analysis*; D003124:Colorimetry; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000201:enzymology*; D007211:Indoles / Q000378:metabolism; D007306:Insecticides / Q000032:analysis*; D009943:Organophosphorus Compounds / Q000032:analysis*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity; D014675:Vegetables / Q000737:chemistry
chemical_list: D002219:Carbamates; D029721:Drosophila Proteins; D007211:Indoles; D007306:Insecticides; D009943:Organophosphorus Compounds; D011994:Recombinant Proteins; C053640:indoxyl acetate; D000110:Acetylcholinesterase