title: Antioxidant activity and protective effect of anthocyanin oligomers on H₂O₂-triggered G2/M arrest in retinal cells.
abstract: In this study, the free-radical-scavenging properties of anthocyanin oligomers for 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, alkyl radical, and hydroxyl radical were evaluated using electron spin resonance (ESR) spectroscopy. The DPPH radical, alkyl radical, and hydroxyl radical scavenging activity of anthocyanin oligomers increased in a dose-dependent manner, with the 50% inhibitory concentration (IC₅₀) value of 13.0, 14.0, and 448.0 μg/mL, respectively. The inhibitory effect of anthocyanin oligomers on lipid peroxidation was examined with ferric thiocyanate (FTC) and thiobarbituric acid (TBA). The inhibitory activity of anthocyanin oligomers was found to be comparable to that of vitamin E. In addition, anthocyanin oligomers enhanced the activities of superoxide dismutase (SOD, EC 1.15.1.1), catalase (CAT, EC 1.11.1.6), glutathione peroxidase (GPx, EC 1.11.1.9), and glutathione-S-transferase (GST, EC 2.5.1.18) in ARPE-19 cells. In addition, anthocyanin oligomers inhibited the H₂O₂-induced G2/M phase arrest in ARPE-19 cells. Taken together, the present results demonstrate that anthocyanin oligomers have high antioxidative activity.
authors.affiliation: Department of Biotechnology, School of Medicine, Konkuk University, Chungju 380-701, Korea.
authors.forename: Jin-Woo
authors.initials: JW
authors.lastname: Hwang
authors.forename: Eun-Kyung
authors.initials: EK
authors.lastname: Kim
authors.forename: Seung-Jae
authors.initials: SJ
authors.lastname: Lee
authors.forename: Yon-Suk
authors.initials: YS
authors.lastname: Kim
authors.forename: Sang-Ho
authors.initials: SH
authors.lastname: Moon
authors.forename: Byong-Tae
authors.initials: BT
authors.lastname: Jeon
authors.forename: Si-Heung
authors.initials: SH
authors.lastname: Sung
authors.forename: Eun-Tae
authors.initials: ET
authors.lastname: Kim
authors.forename: Pyo-Jam
authors.initials: PJ
authors.lastname: Park
mesh_terms: D000872:Anthocyanins / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D002460:Cell Line; D004578:Electron Spin Resonance Spectroscopy; D059565:G2 Phase Cell Cycle Checkpoints / Q000187:drug effects*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D059566:M Phase Cell Cycle Checkpoints / Q000187:drug effects*; D055213:Retinal Pigment Epithelium / Q000166:cytology* / Q000187:drug effects
chemical_list: D000872:Anthocyanins; D000975:Antioxidants; D006861:Hydrogen Peroxide