title: Transcriptome analysis of rosette and folding leaves in Chinese cabbage using high-throughput RNA sequencing.
abstract: In this study, we report the first use of RNA-sequencing to gain insight into the wide range of transcriptional events that are associated with leafy head development in Chinese cabbage. We generated 53.5 million sequence reads (90 bp in length) from the rosette and heading leaves. The sequence reads were aligned to the recently sequenced Chiifu genome and were analyzed to measure the gene expression levels, to detect alternative splicing events and novel transcripts, to determine the expression of single nucleotide polymorphisms, and to refine the annotated gene structures. The analysis of the global gene expression pattern suggests two important concepts, which govern leafy head formation. Firstly, some stimuli, such as carbohydrate levels, light intensity and endogenous hormones might play a critical role in regulating the leafy head formation. Secondly, the regulation of transcription factors, protein kinases and calcium may also be involved in this developmental process.
authors.affiliation: Institute of Vegetables, Shandong Academy of Agricultural Sciences/Shandong Key Laboratory of Greenhouse Vegetable Biology/Shandong Branch of National Vegetable Improvement Center, Jinan 250100, China.
authors.forename: Fengde
authors.initials: F
authors.lastname: Wang
authors.forename: Libin
authors.initials: L
authors.lastname: Li
authors.forename: Huayin
authors.initials: H
authors.lastname: Li
authors.forename: Lifeng
authors.initials: L
authors.lastname: Liu
authors.forename: Yihui
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jianwei
authors.initials: J
authors.lastname: Gao
authors.forename: Xiaowu
authors.initials: X
authors.lastname: Wang
mesh_terms: D017398:Alternative Splicing; D029741:Brassica rapa / Q000033:anatomy & histology / Q000235:genetics*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D018515:Plant Leaves / Q000033:anatomy & histology / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D020133:Reverse Transcriptase Polymerase Chain Reaction