title: GSK3 inhibitor-BIO regulates proliferation of immortalized pancreatic mesenchymal stem cells (iPMSCs).
abstract: BACKGROUND
  The small molecule 6-bromoindirubin-30-oxime (BIO), a glycogen synthase kinase 3 (GSK3) inhibitor, is a pharmacological agent known to maintain self-renewal in human and mouse embryonic stem cells (ESCs). However, the precise role of GSK3 in immortalized pancreatic mesenchymal stem cells (iPMSCs) growth and survival is not completely understood at present.
  
  
  RESULTS
  To determine whether this molecule is involved in controlling the proliferation of iPMSCs, we examined the effect of BIO on iPMSCs. We found that the inactivation of GSK3 by BIO can robustly stimulate iPMSCs proliferation and mass formation as shown by QRT-PCR, western blotting, 5-Bromo-2-deoxyuridine (BrdU) immunostaining assay and tunel assay. However, we did not find the related roles of BIO on β cell differentiation by immunostaining, QRT-PCR assay, glucose-stimulated insulin release and C-peptide content analysis.
  
  
  CONCLUSIONS
  These results suggest that BIO plays a key role in the regulation of cell mass proliferation and maintenance of the undifferentiated state of iPMSCs.
authors.affiliation: College of Veterinary Medicine, Shaanxi Center of Stem Cells Engineering and Technology, Key Lab for Animal Biotechnology of Agriculture Ministry of China, Northwest A & F University, Yangling, Shaanxi, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Cao
authors.forename: Yuankui
authors.initials: Y
authors.lastname: Chu
authors.forename: Xiao
authors.initials: X
authors.lastname: Lv
authors.forename: Pubin
authors.initials: P
authors.lastname: Qiu
authors.forename: Chao
authors.initials: C
authors.lastname: Liu
authors.forename: Huiru
authors.initials: H
authors.lastname: Zhang
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.forename: Sha
authors.initials: S
authors.lastname: Peng
authors.forename: Zhongying
authors.initials: Z
authors.lastname: Dou
authors.forename: Jinlian
authors.initials: J
authors.lastname: Hua
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology; D015820:Cadherins / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D005947:Glucose / Q000378:metabolism; D038362:Glycogen Synthase Kinase 3 / Q000378:metabolism*; D006801:Humans; D007211:Indoles / Q000494:pharmacology*; D007328:Insulin / Q000378:metabolism; D007515:Islets of Langerhans / Q000166:cytology; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods; D010091:Oximes / Q000494:pharmacology*; D010179:Pancreas / Q000166:cytology*; D051176:beta Catenin / Q000378:metabolism
chemical_list: C483321:6-bromoindirubin-3'-oxime; D015820:Cadherins; D007211:Indoles; D007328:Insulin; D010091:Oximes; D051176:beta Catenin; D038362:Glycogen Synthase Kinase 3; D001973:Bromodeoxyuridine; D005947:Glucose