title: Coptisine exert cardioprotective effect through anti-oxidative and inhibition of RhoA/Rho kinase pathway on isoproterenol-induced myocardial infarction in rats.
abstract: OBJECTIVE
  Because myocardial infarction is a major cause of morbidity and mortality worldwide, protecting the heart from the ischemia is the focus of intense research. Coptisine is an isoquinoline alkaloid extracted form Coptidis Rhizoma. This study aims to elucidate if coptisine is responsible for cardioprotection using myocardial infarction (MI) rat models and investigate its potential mechanism of action.
  
  
  METHODS
  Myocardial infarction was produced in rats with 85 mgkg(-1) isoproterenol administered subcutaneously twice at an interval of 24 h. The rats were randomized into 7 groups: (I) Normal; (II) ISO; (III) ISO+fasudil; (IV) ISO+isosorbide dinitrate (ISDN) and (V-VII) ISO+coptisine (25, 50 and 100 mgkg(-1)). Cardiac function and markers of cardiac ischemic were assessed after MI.
  
  
  RESULTS
  Rats pretreated with coptisine (25, 50 and 100 mgkg(-1)) for 21 days and received subcutaneously injected with ISO (85 mgkg(-1)) on the 20th and 21st day at an interval of 24 h. The results suggested that coptisine has strong antioxidant activity, and it can maintain cell membrane integrity, ameliorate mitochondrial respiratory dysfunction, reduce myocardial cells apoptosis, inhibit RhoA/ROCK expression induced by high-dose isoproterenol administration.
  
  
  CONCLUSIONS
  Coptisine provided cardioprotection in a model of myocardial infarction, and therefore should be considered as a novel adjunctive therapy for attenuating myocardial damage.
authors.affiliation: State Key Laboratory for Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Science & Peking Union Medical College, Beijing 100050, China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Gong
authors.forename: Lian-Hua
authors.initials: LH
authors.lastname: Fang
authors.forename: Su-Bo
authors.initials: SB
authors.lastname: Wang
authors.forename: Jia-Lin
authors.initials: JL
authors.lastname: Sun
authors.forename: Hai-Lin
authors.initials: HL
authors.lastname: Qin
authors.forename: Xiao-Xiu
authors.initials: XX
authors.lastname: Li
authors.forename: Shou-Bao
authors.initials: SB
authors.lastname: Wang
authors.forename: Guan-Hua
authors.initials: GH
authors.lastname: Du
mesh_terms: D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D001599:Berberine / Q000031:analogs & derivatives* / Q000627:therapeutic use; D002316:Cardiotonic Agents / Q000627:therapeutic use*; D007545:Isoproterenol; D008297:Male; D008929:Mitochondria, Heart / Q000187:drug effects; D009203:Myocardial Infarction / Q000139:chemically induced / Q000517:prevention & control*; D051381:Rats; D017207:Rats, Sprague-Dawley; D054460:rho-Associated Kinases / Q000037:antagonists & inhibitors; D020742:rhoA GTP-Binding Protein / Q000037:antagonists & inhibitors
chemical_list: D000975:Antioxidants; D002316:Cardiotonic Agents; C034384:coptisine; D001599:Berberine; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D054460:rho-Associated Kinases; D020742:rhoA GTP-Binding Protein; D007545:Isoproterenol; C049347:fasudil