title: [Study on double fingerprints of isatidis radix micropowder].
abstract: OBJECTIVE
  To establish the double HPLC fingerprints of water-soluble composition and amino acids precolumn derivative reagent of 13 batches of Isatidis Radix micropower.
  
  
  METHODS
  The gradient elution was adopted with Hypersil BDS C18 column (4.6 mm x 250 mm, 5 microm). Water-soluble ingredients were detected with acetonitrile-0.1% phosphoric acid solution as mobile phase, flow rate 0.5 mL x min(-1), column temperature 20 degrees C, and the injection volume 10 microL. Amino acid ingredient were derived by PITC, and then were detected with mobile phase of 0.1 mol x L(-1) sodium acetate buffer solution (pH 6.5) - acetonitrile, flow rate 1.0 mL x min(-1), column temperature 30 degrees C, and the injection volume 5 microL. Both of the absorption wavelengths were 254 nm. Pharmacopoeia Commission "Chinese chromatographic fingerprint evaluation system (version 2.0)" was adopted to analyse, fingerprints of Isatidis Radix micropower were established, at the same time 4 main ingredients were recognized by the SPSS cluster analysis.
  
  
  RESULTS
  Common mode of Fingerprint to water-soluble and amino acids ingredient of Isatidis Radix micropower was established, then adenosine, epigoitrin and 15 amino acids were identified as characteristic peaks. Cluster analysis showed that different kinds of the herbal Isatidis Radix micropower from different areas were different levels in the main ingredients.
  
  
  CONCLUSIONS
  Double fingerprints of Isatidis Radix micropower is established. Each peak is optimally separated in chromatogram, which provides a scientific basis for quality control of Isatidis Radix micropower.
authors.affiliation: Hunan University of Chinese Medicine, Changsha 410013, China. fengxiaoyan1127@163.com
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Feng
authors.forename: Shuihan
authors.initials: S
authors.lastname: Zhang
authors.forename: Guangxian
authors.initials: G
authors.lastname: Cai
authors.forename: Yike
authors.initials: Y
authors.lastname: Huang
authors.forename: Yi
authors.initials: Y
authors.lastname: Shao
mesh_terms: D000596:Amino Acids / Q000032:analysis; D031219:Isatis / Q000737:chemistry*; D011208:Powders; D011786:Quality Control
chemical_list: D000596:Amino Acids; D011208:Powders