title: Microarray expression profiling in adhesion and normal peritoneal tissues.
abstract: OBJECTIVE
  To identify molecular markers associated with adhesion and normal peritoneal tissue using microarray expression profiling.
  
  
  METHODS
  Comparative study.
  
  
  METHODS
  University hospital.
  
  
  METHODS
  Five premenopausal women.
  
  
  METHODS
  Adhesion and normal peritoneal tissue samples were obtained from premenopausal women. Ribonucleic acid was extracted using standard protocols and processed for hybridization to Affymetrix Whole Transcript Human Gene Expression Chips. Microarray data were obtained from five different patients, each with adhesion tissue and normal peritoneal samples. Real-time polymerase chain reaction was performed for confirmation using standard protocols.
  
  
  METHODS
  Gene expression in postoperative adhesion and normal peritoneal tissues.
  
  
  RESULTS
  A total of 1,263 genes were differentially expressed between adhesion and normal tissues. One hundred seventy-three genes were found to be up-regulated and 56 genes were down-regulated in the adhesion tissues compared with normal peritoneal tissues. The genes were sorted into functional categories according to Gene Ontology annotations. Twenty-six up-regulated genes and 11 down-regulated genes were identified with functions potentially relevant to the pathophysiology of postoperative adhesions. We evaluated and confirmed expression of 12 of these specific genes via polymerase chain reaction.
  
  
  CONCLUSIONS
  The pathogenesis, natural history, and optimal treatment of postoperative adhesive disease remains unanswered. Microarray analysis of adhesions identified specific genes with increased and decreased expression when compared with normal peritoneum. Knowledge of these genes and ontologic pathways with altered expression provide targets for new therapies to treat patients who have or are at risk for postoperative adhesions.
authors.affiliation: Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Geisinger Medical Center, Danville, Pennsylvania, USA. dambler@drstorment.com
authors.forename: Dana R
authors.initials: DR
authors.lastname: Ambler
authors.forename: Alicia M
authors.initials: AM
authors.lastname: Golden
authors.forename: Jennifer S
authors.initials: JS
authors.lastname: Gell
authors.forename: Ghassan M
authors.initials: GM
authors.lastname: Saed
authors.forename: David J
authors.initials: DJ
authors.lastname: Carey
authors.forename: Michael P
authors.initials: MP
authors.lastname: Diamond
mesh_terms: D000328:Adult; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation; D005819:Genetic Markers; D006785:Hospitals, University; D006801:Humans; D007247:Infertility, Female / Q000601:surgery*; D010535:Laparoscopy / Q000009:adverse effects*; D020411:Oligonucleotide Array Sequence Analysis; D010532:Peritoneal Diseases / Q000209:etiology / Q000235:genetics* / Q000473:pathology; D010537:Peritoneum / Q000737:chemistry / Q000601:surgery*; D011183:Postoperative Complications / Q000209:etiology / Q000235:genetics* / Q000473:pathology; D017697:Premenopause; D012333:RNA, Messenger / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000267:Tissue Adhesions
chemical_list: D005819:Genetic Markers; D012333:RNA, Messenger