title: [Influence of mesenchymal stem cells on proliferation of HL-60].
abstract: The objective of this study was to explore the effect of mesenchymal stem cells (MSC) on HL-60 proliferation and its molecular mechanism. HL-60 cells co-cultivated with MSC were used as experiment group, and the cells cultivated solely were taken as control group. HL-60 cells in the two groups were counted at different time. The time-quantity curve was drawn. The cell cycle and apoptosis ratio of HL-60 cells were compared between the two groups and expressions of Survivin and Bcl-2 protein were detected by flow cytometry. The results showed that HL-60 cells cultivated with MSC were obviously inhibited, especially on day 5 and 7 (P < 0.01). HL-60 cells were distributed on the phase of G(0)/G(1) [control group (47.0 ± 9.0)% vs experiment group (70.0 ± 16.0)%, P = 0.003], and apoptotic peak appeared. Both of Survivin and Bcl-2 protein expressions in HL-60 cells decreased [Bcl-2 protein in control group (63.0 ± 9.1)% vs experiment group (50.0 ± 14.1)%, P = 0.045; Survivin in control group (94.0 ± 9.3)% vs experiment group (77.0 ± 11.8)%, P = 0.006]. It is concluded that the MSC can inhibit HL-60 cell proliferation, and promote HL-60 cell apoptosis.
authors.affiliation: Department of Hematology, PLA Navy General Hospital, Beijing, China.
authors.forename: Wen-Jie
authors.initials: WJ
authors.lastname: Yin
authors.forename: Xiao-Peng
authors.initials: XP
authors.lastname: Liu
authors.forename: You-Zhang
authors.initials: YZ
authors.lastname: Huang
authors.forename: Ping-Di
authors.initials: PD
authors.lastname: Yang
mesh_terms: D000328:Adult; D017209:Apoptosis; D001854:Bone Marrow Cells / Q000166:cytology*; D049109:Cell Proliferation; D018920:Coculture Techniques; D005434:Flow Cytometry; D018922:HL-60 Cells; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008875:Middle Aged; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D000077022:Survivin
chemical_list: C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D000077022:Survivin