title: Heterologous expression of bacterial nitric oxide synthase gene: a potential biological method to control biofilm development in the environment.
abstract: The nitric oxide synthase gene from  Bacillus subtilis  was heterologously expressed in  Pseudomonas putida  using a broad-host expression vector. Both the performance of the nitric oxide-specific fluorescent probe and the quantitative assessment of the nitric oxide end products demonstrated the generation of nitric oxide. The endogenous nitric oxide increased the motility of P. putida and decreased the capacity of P. putida and other multispecies bacteria to develop biofilms. On the practical side, endogenous nitric oxide offers an advantage in generating continuous, controllable levels of nitric oxide, which suggests a new method to use nitric oxide in the control of biofilms.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
authors.forename: Pulin
authors.initials: P
authors.lastname: Liu
authors.forename: Qiaoyun
authors.initials: Q
authors.lastname: Huang
authors.forename: Wenli
authors.initials: W
authors.lastname: Chen
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics; D018407:Bacterial Physiological Phenomena; D018441:Biofilms; D004783:Environmental Microbiology; D015964:Gene Expression Regulation, Bacterial; D009569:Nitric Oxide / Q000378:metabolism; D020030:Nitric Oxide Donors; D019001:Nitric Oxide Synthase / Q000235:genetics* / Q000378:metabolism*; D016958:Pseudomonas putida / Q000201:enzymology / Q000235:genetics / Q000502:physiology*
chemical_list: D020030:Nitric Oxide Donors; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase