title: Hemostatically distinct FFPs equally improve abnormal TEG variables in an in vitro dilutional coagulopathy model.
abstract: BACKGROUND
  To improve fresh frozen plasma (FFP) availability, thawed plasma is stored at 4°C for up to 5 days and considered equivalent to freshly thawed FFP. However, we have shown that hemostatic potential of thawed plasma is highly variable between donors and significantly diminished during storage. We hypothesized that smaller volumes of plasma with higher hemostatic potential (FFP-H) would be needed to restore normal thrombelastogram (TEG) values compared to plasma with lower hemostatic potential (FFP-L).
  
  
  METHODS
  A dilutional coagulopathy model was established from whole blood by diluting plasma with saline to 23%, while cellular components were kept unchanged. Saline was gradually replaced with equal volumes of FFPs with distinctive hemostatic potentials, which was evaluated by the calibrated automated thrombogram. Clot formation in the presence of tissue factor was evaluated by TEG at baseline and after addition of increasing concentrations of FFP-H and FFP-L.
  
  
  RESULTS
  Blood dilution with saline in the presence of tissue factor resulted in abnormal TEGs that resemble a pattern observed in severely bleeding trauma patients. All FFPs produced similar improvements in TEG variables despite different hemostatic potentials. TEG changes were solely dependent on FFP volume and reached the normal reference range when plasma concentration increased to 40%.
  
  
  CONCLUSIONS
  Plasma dilution and tissue factor in whole blood results in an abnormal TEG with a hyperfibrinolytic pattern. A plasma concentration of at least 40% was necessary for TEG normalization after dilution with saline. An effect of FFPs' hemostatic potential on clot formation could not be detected by TEG in this in vitro model.
authors.affiliation: Department of Surgery, and Center for Translational Injury Research, The University of Texas Health Science Center at Houston, Houston, TX 77030, usa.
authors.forename: Vadim
authors.initials: V
authors.lastname: Kostousov
authors.forename: Yao-Wei W
authors.initials: YW
authors.lastname: Wang
authors.forename: Charles E
authors.initials: CE
authors.lastname: Wade
authors.forename: John B
authors.initials: JB
authors.lastname: Holcomb
authors.forename: Bryan A
authors.initials: BA
authors.lastname: Cotton
authors.forename: Nena
authors.initials: N
authors.lastname: Matijevic
mesh_terms: D001778:Blood Coagulation Disorders / Q000097:blood*; D006487:Hemostasis; D006801:Humans; D010949:Plasma / Q000502:physiology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013916:Thrombelastography