title: Enzymatic detergent in rat peritoneum. A comparative study with physiological solution.
abstract: BACKGROUND
  Peritoneal washing out with physiological solution with different substances added is useful in peritoneal infections, but the effect of enzymatic detergents, such as quaternary didecyl-dimethyl ammonium compounds (DDAC), used in the sterilization of surgical material is unknown. We undertook this study to determine histological changes (inflammation, fibrosis and new vessel formation) in the peritoneum of Wistar rats after the application of physiological solution or DDAC.
  
  
  METHODS
  The minimum inhibitory concentration (MIC) of DDAC for E. coli (512 μg/ml) and E. faecalis (128 μg/ml) was determined. Sixty-three Wistar rats weighing 200 ± 20 g were studied. They were divided into three groups: control: 7 rats were instilled with 3 ml of physiological solution in peritoneal cavity; groups 1 and 2 were instilled with 3 ml of MIC for E. coli and E. faecalis, respectively. These groups were divided into four subgroups of seven animals. In every rat, 1 cm(2) of peritoneum was obtained at 2, 7, 14, and 21 days for histological study with hematoxylin-eosin. Ten fields were evaluated. The data obtained were analyzed with the Mann-Whitney test.
  
  
  RESULTS
  There were no significant differences in inflammation, fibrosis and new vessel formation with the physiological solution vs. DDAC at 2, 7, 14, and 21 days (p >0.05), except for inflammation at 2 days in group 2 (p = 0.026), which remitted.
  
  
  CONCLUSIONS
  There was no significant difference in changes in rat peritoneum after physiological solution or DDAC application.
authors.affiliation: Hospital Médica TEC 100, Querétaro, México. rodoldoc@gmail.com
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Arteaga-Torres
authors.forename: Minerva
authors.initials: M
authors.lastname: Escartín-Chávez
authors.forename: César
authors.initials: C
authors.lastname: Gutiérrez-Samperio
authors.forename: Juan Manuel
authors.initials: JM
authors.lastname: Ruiz-Acosta
authors.forename: Marco
authors.initials: M
authors.lastname: Alonso-Gallegos
authors.forename: Ricardo
authors.initials: R
authors.lastname: Lerma-Alvarado
mesh_terms: D000818:Animals; D003902:Detergents / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use* / Q000633:toxicity; D004353:Drug Evaluation, Preclinical; D013293:Enterococcus faecalis / Q000187:drug effects*; D004848:Epithelium / Q000187:drug effects / Q000473:pathology; D004926:Escherichia coli / Q000187:drug effects*; D004927:Escherichia coli Infections / Q000188:drug therapy*; D005260:Female; D016908:Gram-Positive Bacterial Infections / Q000188:drug therapy*; D007322:Instillation, Drug; D008297:Male; D008826:Microbial Sensitivity Tests; D009389:Neovascularization, Pathologic / Q000188:drug therapy / Q000209:etiology / Q000517:prevention & control; D056627:Peritoneal Fibrosis / Q000188:drug therapy / Q000209:etiology / Q000517:prevention & control; D010537:Peritoneum / Q000098:blood supply / Q000187:drug effects* / Q000473:pathology; D010538:Peritonitis / Q000150:complications / Q000188:drug therapy* / Q000382:microbiology; D000644:Quaternary Ammonium Compounds / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use* / Q000633:toxicity; D011897:Random Allocation; D017208:Rats, Wistar; D012965:Sodium Chloride / Q000494:pharmacology; D007507:Therapeutic Irrigation
chemical_list: D003902:Detergents; D000644:Quaternary Ammonium Compounds; C053645:N,N-dimethyl-N-hexadecyl-1-octadecylammonium; D012965:Sodium Chloride