title: First report on interferon related developmental regulator-1 from Macrobrachium rosenbergii: bioinformatic analysis and gene expression.
abstract: This study reports the first full length gene of interferon related developmental regulator-1 (designated as MrIRDR-1), identified from the transcriptome of Macrobrachium rosenbergii. The complete gene sequence of the MrIRDR-1 is 2459 base pair long with an open reading frame of 1308 base pairs and encoding a predicted protein of 436 amino acids with a calculated molecular mass of 48 kDa. The MrIRDR-1 protein contains a long interferon related developmental regulator super family domain between 30 and 330. The mRNA expressions of MrIRDR-1 in healthy and the infectious hypodermal and hematopoietic necrosis virus (IHHNV) infected M. rosenbergii were examined using qRT-PCR. The MrIRDR-1 is highly expressed in hepatopancreas along with all other tissues (walking leg, gills, muscle, haemocyte, pleopods, brain, stomach, intestine and eye stalk). After IHHNV infection, the expression is highly upregulated in hepatopancreas. This result indicates an important role of MrIRDR-1 in prawn defense system.
authors.affiliation: Centre for Biotechnology in Agriculture Research, Division of Genetics & Molecular Biology, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Jesu
authors.initials: J
authors.lastname: Arockiaraj
authors.forename: Sarasvathi
authors.initials: S
authors.lastname: Easwvaran
authors.forename: Puganeshwaran
authors.initials: P
authors.lastname: Vanaraja
authors.forename: Arun
authors.initials: A
authors.lastname: Singh
authors.forename: Rofina Yasmin
authors.initials: RY
authors.lastname: Othman
authors.forename: Subha
authors.initials: S
authors.lastname: Bhassu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019295:Computational Biology; D018076:DNA, Complementary / Q000235:genetics / Q000276:immunology; D019223:Densovirinae / Q000276:immunology*; D020869:Gene Expression Profiling / Q000662:veterinary; D005786:Gene Expression Regulation; D017874:Immediate-Early Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D033541:Palaemonidae / Q000235:genetics* / Q000276:immunology*; D010802:Phylogeny; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment / Q000662:veterinary; D020539:Sequence Analysis, Protein / Q000662:veterinary; D017385:Sequence Homology; D014018:Tissue Distribution
chemical_list: D018076:DNA, Complementary; D017874:Immediate-Early Proteins