title: [Effects of Schistosoma 22.6 kDa/26 GST molecule mixed with Sepharose 4B beads on induction of CD4+CD25+ regulatory T cells].
abstract: OBJECTIVE
  To study the responses of regulatory T cells induced by Sepharose 4B beads mixed antigen.
  
  
  METHODS
  Mice were immunized with different dosages of antigens, and the proportions of regulatory T cells were examined by flow cytometry. The 3H-thymidin incorporation method was used to detect the inhibitory function of Tregs. In vitro, dendritic cells were pulsed with different antigens. The expressions of MHC II, CD40, CD80/86 molecules on dendritic cells and the proportions of regulatory T cells were examined by flow cytometry. The 3H-thymidin incorporation method was used for the determination of Tregs function.
  
  
  RESULTS
  Compared with control groups, the proportion of regulatory T cells in mice immunized with Sepharose 4B beads mixed antigen was(11.48 +/- 4.12)%, and the difference was significant (P < 0.05). The inhibition of regulatory T cells in mice immunized with Sepharose 4B beads mixed antigen showed a stronger potential (cpm was 720 +/- 180.4). Meanwhile, the proportions of regulatory T cells were (17.0 +/- 80.57)% and (30.14 +/- 3.62)% when the CD4+ T cells were co-cultured with dendritic cells pulsed with Sepharose 4B beads mixed rSj22.6/26GST and OVA, respectively.
  
  
  CONCLUSIONS
  Sepharose 4B beads mixed antigen could induce the regulatory T cells in vivo and in vitro.
authors.affiliation: Department of Pathogenic Biology, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhou
authors.forename: Lei
authors.initials: L
authors.lastname: Shi
authors.forename: Dan
authors.initials: D
authors.lastname: Zheng
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology; D005260:Female; D005982:Glutathione Transferase / Q000235:genetics / Q000276:immunology*; D015801:Helminth Proteins / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007114:Immunization; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D012547:Schistosoma / Q000276:immunology* / Q000469:parasitology; D012685:Sepharose / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
chemical_list: D015801:Helminth Proteins; D053645:Interleukin-2 Receptor alpha Subunit; D011993:Recombinant Fusion Proteins; D012685:Sepharose; D005982:Glutathione Transferase