title: RNA interference links oxidative stress to the inhibition of heat stress adaptation.
abstract: Increased oxidative stress is associated with various diseases and aging, while adaptation to heat stress is an important determinant of survival and contributes to longevity. However, the impact of oxidative stress on heat resistance remains largely unclear.
  
  
  OBJECTIVE
  In this study we investigated how oxidative stress impinges on heat stress responses.
  
  
  RESULTS
  We report that hydrogen-peroxide (H(2)O(2)) pretreatment inhibits both acquired thermotolerance and heat-induced Hsp70 expression in mammalian cells, as well as acquired thermotolerance in the nematode Caenorhabditis elegans, via RNA interference. Moreover, we demonstrate that elimination of RNA interference by silencing key enzymes in microRNA biogenesis, dcr-1 or pash-1, restores the diminished intrinsic thermotolerance of aged and H(2)O(2)-elimination compromised (catalase-2 and peroxiredoxin-2 deficient) worms.
  
  
  CONCLUSIONS
  These results uncover a novel post-transcriptional element in the regulation of heat stress adaptation under oxidative conditions that may have implications in disease susceptibility and aging.
authors.affiliation: Department of Medical Chemistry, Semmelweis University, Budapest, Hungary.
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Spiró
authors.forename: Mehmet Alper
authors.initials: MA
authors.lastname: Arslan
authors.forename: Milán
authors.initials: M
authors.lastname: Somogyvári
authors.forename: Minh Tu
authors.initials: MT
authors.lastname: Nguyen
authors.forename: Arne
authors.initials: A
authors.lastname: Smolders
authors.forename: Balázs
authors.initials: B
authors.lastname: Dancsó
authors.forename: Nóra
authors.initials: N
authors.lastname: Németh
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Elek
authors.forename: Bart P
authors.initials: BP
authors.lastname: Braeckman
authors.forename: Péter
authors.initials: P
authors.lastname: Csermely
authors.forename: Csaba
authors.initials: C
authors.lastname: Sőti
mesh_terms: D000818:Animals; D019556:COS Cells; D017173:Caenorhabditis elegans / Q000378:metabolism / Q000502:physiology; D002522:Chlorocebus aethiops; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D018869:Heat-Shock Response / Q000187:drug effects / Q000235:genetics; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008856:Microscopy, Fluorescence; D018384:Oxidative Stress / Q000187:drug effects* / Q000235:genetics*; D034622:RNA Interference / Q000502:physiology*
chemical_list: D018840:HSP70 Heat-Shock Proteins; D006861:Hydrogen Peroxide