title: Detection of serum anti-P53 antibodies from patients with colorectal cancer in China using a combination of P53- and phage-ELISA: correlation to clinical parameters.
abstract: BACKGROUND
  Colorectal cancer is one of the most common malignant tumors in China. The aims of this research were to increase the sensitivity of anti-p53 antibody detection in the sera of patients with colorectal cancer and to assist in their diagnosis.
  
  
  METHODS
  Sixty-seven non-selected Chinese with colorectal cancer were involved in this study. Anti-p53 antibodies in serum were detected by ELISA using recombinant human wild- type p53 protein and hybrid phage as the coating antigen. Correlations between the anti-p53 antibodies and clinicopathological parameters were also analyzed.
  
  
  RESULTS
  The detection efficiency of anti-p53 antibodies in the patients with colorectal cancer was increased (46.3%, 31/67) through the combination of the two ELISA methods compared with each method alone. The titer of serum anti-p53 antibodies was not associated with clinicopathological parameters, but there was a significant correlation between their presence, the CEA level, and the stage of the patient's colorectal cancer.
  
  
  CONCLUSIONS
  These results demonstrate that combination of the two ELISA methods increased the detection rate of anti-p53 antibodies in patients with colorectal cancer. This research may provide a useful method to complement conventional clinical diagnosis.
authors.affiliation: Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, Changchun, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wu
authors.forename: Tian
authors.initials: T
authors.lastname: Qiu
authors.forename: Pengtao
authors.initials: P
authors.lastname: Pan
authors.forename: Dehai
authors.initials: D
authors.lastname: Yu
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Ju
authors.forename: Xuewei
authors.initials: X
authors.lastname: Qu
authors.forename: Xiang
authors.initials: X
authors.lastname: Gao
authors.forename: Chuanbin
authors.initials: C
authors.lastname: Mao
authors.forename: Li
authors.initials: L
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000906:Antibodies / Q000097:blood* / Q000276:immunology; D000912:Antibodies, Neoplasm / Q000097:blood / Q000276:immunology; D014408:Biomarkers, Tumor / Q000097:blood / Q000276:immunology; D002272:Carcinoembryonic Antigen / Q000097:blood / Q000276:immunology; D015179:Colorectal Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011994:Recombinant Proteins / Q000276:immunology; D016159:Tumor Suppressor Protein p53 / Q000276:immunology*; D055815:Young Adult
chemical_list: D000906:Antibodies; D000912:Antibodies, Neoplasm; D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; D011994:Recombinant Proteins; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53