title: Identification of olive (Olea europaea) seed and pulp proteins by nLC-MS/MS via combinatorial peptide ligand libraries.
abstract: Different types of extraction protocols are described for identifying proteins in seed and pulp of olive (Olea europea), by employing both conventional extraction methods and capture with ProteoMiner as well as with in house-made combinatorial peptide ligand libraries (HM-CPLLs) at pH 7.4 and at pH 2.2. Thanks to the use of CPLLs, able to dramatically amplify the signal of low-abundance species, a quite large number of compounds has been indeed identified: 61 in the seed (vs. only four reported in current literature) and 231 in the pulp (vs. 56 described so far), the deepest investigation up to the present of the olive proteome. In the seed, it highlights the presence of seed storage proteins, oleosins and histones. In the pulp, the allergenic thaumatin-like protein (Ole e 13) was confirmed, among the other 231, as the most abundant protein in the olive pulp. The present research has also been undertaken with the aim of identifying proteins in olive oil and ascertaining the relative contribution of seed and pulp proteins in their presence, if any, in oils.
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, University of Alcalá, Ctra. Madrid-Barcelona, Km. 33.600, E-28871 Alcalá de Henares, Madrid, Spain.
authors.forename: Clara
authors.initials: C
authors.lastname: Esteve
authors.forename: Alfonsina
authors.initials: A
authors.lastname: D'Amato
authors.forename: María Luisa
authors.initials: ML
authors.lastname: Marina
authors.forename: María Concepción
authors.initials: MC
authors.lastname: García
authors.forename: Attilio
authors.initials: A
authors.lastname: Citterio
authors.forename: Pier Giorgio
authors.initials: PG
authors.lastname: Righetti
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D005638:Fruit / Q000737:chemistry / Q000378:metabolism; D008024:Ligands; D008954:Models, Biological; D031658:Olea / Q000737:chemistry* / Q000378:metabolism; D019151:Peptide Library; D010940:Plant Proteins / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D012639:Seeds / Q000737:chemistry / Q000378:metabolism; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D008024:Ligands; D019151:Peptide Library; D010940:Plant Proteins