title: Temperature-responsive poly(N-isopropylacrylamide)-grafted microcarriers for large-scale non-invasive harvest of anchorage-dependent cells.
abstract: Cell cultivation on the surface of microcarriers in stirred suspension is an essential method for the large-scale culture of anchorage-dependent cells. For applying this method to the field of cell therapy and for obtaining a large number of intact cells, non-invasive cell harvest without proteolytic enzyme treatment is an advantageous method. In this regard, temperature-responsive microcarriers that bearing poly(N-isopropylacrylamide) (PIPAAm)-grafted chains on the outermost surface were developed for harvesting cultured cells by temperature alteration. PIPAAm-grafted beads with the various grafted amount of PIPAAm and various bead diameters were synthesized for optimizing cell proliferation and thermally-induced detachment on the surface. The chinese hamster ovary (CHO-K1) cells adhered on the surface of all PIPAAm-grafted beads at 37 °C, while the adhering cells were found to detach themselves from the surfaces at 20 °C. The efficiency of thermally-induced cell detachment increased with increasing the grafted amount of PIPAAm and the diameter of bead. An efficient cell proliferation on bead surfaces in stirred suspension culture and subsequent thermally-induced cell detachment were achieved by the precise regulation of both the grafted amount of PIPAAm and diameter of bead. The temperature-responsive microcarriers exhibiting temperature-dependent cell adhesion and detachment will be an attractive candidate for the large-scale cell culture of therapeutic cells.
authors.affiliation: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, and Global Center of Excellence (COE), 8-1 Kawadacho, Shinjuku, Tokyo 162-8666, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Tamura
authors.forename: Jun
authors.initials: J
authors.lastname: Kobayashi
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yamato
authors.forename: Teruo
authors.initials: T
authors.lastname: Okano
mesh_terms: D000178:Acrylamides / Q000138:chemical synthesis / Q000737:chemistry*; D000180:Acrylic Resins; D000818:Animals; D016466:CHO Cells; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D002470:Cell Survival; D006224:Cricetinae; D003412:Cricetulus; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D056951:Photoelectron Spectroscopy; D058105:Polymerization; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry; D013499:Surface Properties; D013696:Temperature; D013997:Time Factors; D014867:Water / Q000737:chemistry
chemical_list: D000178:Acrylamides; D000180:Acrylic Resins; D011108:Polymers; D011137:Polystyrenes; D014867:Water; C052970:poly-N-isopropylacrylamide