title: Comparison of myofibroblasts expression in oral squamous cell carcinoma, verrucous carcinoma, high risk epithelial dysplasia, low risk epithelial dysplasia and normal oral mucosa.
abstract: The aim was to evaluate and compare the presence of myofibroblasts in oral squamous cell carcinoma (OSCC), verrucous carcinoma (VC), high-risk epithelial dysplasia (HRED), low-risk epithelial dysplasia (LRED), and normal oral mucosa (NOM). The study consisted of 37 OSCC, 15 VC, 15 HRED, 15 LRED and 15 NOM. α-smooth muscle actin (α-SMA) antibody was used to identify myofibroblasts. The α-SMA expression was not observed in NOM and LRED. The α-SMA was expressed in 97.29% of OSCC, 86.66% of VC, 46.66 % of HRED. The α-SMA expression was significantly higher in OSCC than VC (p = 0.023) and HRED (p < 0.000). The α-SMA expression was significantly higher in VC than HRED (p = 0.043). Myofibroblastic expression, as highlighted by α-SMA, is undetectable in NOM and LRED but increases as the disease progresses from potentially malignant disorders, as HRED to VC to invasive OSCC. Thus, proliferation of myofibroblasts may be used as a stromal marker of oral premalignancy and malignancy.
authors.affiliation: Department of Oral and Maxillofacial Pathology and Microbiology, Sharad Pawar Dental College and Hospital, Datta Meghe Institute of Medical Sciences, Sawangi (Meghe), Wardha, 442001, Maharashtra, India.
authors.forename: Minal
authors.initials: M
authors.lastname: Chaudhary
authors.forename: Amol Ramchandra
authors.initials: AR
authors.lastname: Gadbail
authors.forename: Gaurav
authors.initials: G
authors.lastname: Vidhale
authors.forename: Mugdha P
authors.initials: MP
authors.lastname: Mankar Gadbail
authors.forename: Shailesh M
authors.initials: SM
authors.lastname: Gondivkar
authors.forename: Madhuri
authors.initials: M
authors.lastname: Gawande
authors.forename: Swati
authors.initials: S
authors.lastname: Patil
mesh_terms: D000199:Actins / Q000032:analysis; D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D018289:Carcinoma, Verrucous / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000473:pathology; D009062:Mouth Neoplasms / Q000473:pathology*; D058628:Myofibroblasts / Q000473:pathology*; D011230:Precancerous Conditions / Q000473:pathology*; D012307:Risk Factors
chemical_list: C541116:ACTA2 protein, human; D000199:Actins