title: Effect of temperature on albumin cobalt binding and its influence on ischemia-modified albumin levels in patients with suspected acute coronary syndrome.
abstract: BACKGROUND
  Ischemia-modified albumin (IMA) has been shown to be a rapidly rising and sensitive biochemical marker especially for the diagnosis of myocardial ischemia. The aim of this study was to evaluate the influence of temperature on the capacity of cobalt binding to human albumin and the influence of this variable on IMA measurement.
  
  
  METHODS
  The following temperatures of incubation were tested for human albumin standard 25 degrees C, 28 degrees C, 31 degrees C, 34 degrees C, and 37 degrees C and for patients with suspected acute coronary syndrome, room temperature and 37 degrees C. IMA was measured by cobalt-albumin binding assay.
  
  
  RESULTS
  There was a strong positive correlation (r = 0.98, p < 0.001) between IMA and the assay temperatures. IMA levels were 0.68 +/- 0.25 absorbance units (ABSU) at room temperature and 0.92 +/- 0.33 ABSU (p < 0.001) at 37 degrees C in the study participants.
  
  
  CONCLUSIONS
  IMA values were influenced by the assay temperature.
authors.affiliation: Laboratório de Bioquímica Clínica, Departamento de Análises Clínicas e Toxicológicas, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Bruna S
authors.initials: BS
authors.lastname: Hausen
authors.forename: Cristiane
authors.initials: C
authors.lastname: Signor
authors.forename: Helena
authors.initials: H
authors.lastname: Kober
authors.forename: Etiane
authors.initials: E
authors.lastname: Tatsch
authors.forename: Renata S
authors.initials: RS
authors.lastname: Pereira
authors.forename: Thiago
authors.initials: T
authors.lastname: Duarte
authors.forename: Carine L
authors.initials: CL
authors.lastname: Hermes
authors.forename: Marta M M F
authors.initials: MM
authors.lastname: Duarte
authors.forename: Rafael N
authors.initials: RN
authors.lastname: Moresco
mesh_terms: D054058:Acute Coronary Syndrome / Q000175:diagnosis* / Q000378:metabolism; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D003035:Cobalt / Q000378:metabolism*; D003951:Diagnostic Errors / Q000517:prevention & control*; D006358:Hot Temperature; D006801:Humans; D011485:Protein Binding; D012680:Sensitivity and Specificity; D012709:Serum Albumin / Q000378:metabolism*; D000075462:Serum Albumin, Human
chemical_list: D015415:Biomarkers; D012709:Serum Albumin; C561522:ischemia-modified albumin; D003035:Cobalt; D000075462:Serum Albumin, Human