title: MALDI imaging mass spectrometry reveals COX7A2, TAGLN2 and S100-A10 as novel prognostic markers in Barrett's adenocarcinoma.
abstract: To characterize proteomic changes found in Barrett's adenocarcinoma and its premalignant stages, the proteomic profiles of histologically defined precursor and invasive carcinoma lesions were analyzed by MALDI imaging MS. For a primary proteomic screening, a discovery cohort of 38 fresh frozen Barrett's adenocarcinoma patient tissue samples was used. The goal was to find proteins that might be used as markers for monitoring cancer development as well as for predicting regional lymph node metastasis and disease outcome. Using mass spectrometry for protein identification and validating the results by immunohistochemistry on an independent validation set, we could identify two of 60 differentially expressed m/z species between Barrett's adenocarcinoma and the precursor lesion: COX7A2 and S100-A10. Furthermore, among 22 m/z species that are differentially expressed in Barrett's adenocarcinoma cases with and without regional lymph node metastasis, one was identified as TAGLN2. In the validation set, we found a correlation of the expression levels of COX7A2 and TAGLN2 with a poor prognosis while S100-A10 was confirmed by multivariate analysis as a novel independent prognostic factor in Barrett's adenocarcinoma. Our results underscore the high potential of MALDI imaging for revealing new biologically significant molecular details from cancer tissues which might have potential for clinical application. This article is part of a Special Issue entitled: Translational Proteomics.
authors.affiliation: Institute of Pathology, Helmholtz Zentrum München - German Research Center for Environmental Health, Neuherberg, Germany.
authors.forename: Mareike
authors.initials: M
authors.lastname: Elsner
authors.forename: Sandra
authors.initials: S
authors.lastname: Rauser
authors.forename: Stefan
authors.initials: S
authors.lastname: Maier
authors.forename: Cédrik
authors.initials: C
authors.lastname: Schöne
authors.forename: Benjamin
authors.initials: B
authors.lastname: Balluff
authors.forename: Stephan
authors.initials: S
authors.lastname: Meding
authors.forename: Gerhard
authors.initials: G
authors.lastname: Jung
authors.forename: Martin
authors.initials: M
authors.lastname: Nipp
authors.forename: Hakan
authors.initials: H
authors.lastname: Sarioglu
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Maccarrone
authors.forename: Michaela
authors.initials: M
authors.lastname: Aichler
authors.forename: Annette
authors.initials: A
authors.lastname: Feuchtinger
authors.forename: Rupert
authors.initials: R
authors.lastname: Langer
authors.forename: Uta
authors.initials: U
authors.lastname: Jütting
authors.forename: Marcus
authors.initials: M
authors.lastname: Feith
authors.forename: Bernhard
authors.initials: B
authors.lastname: Küster
authors.forename: Marius
authors.initials: M
authors.lastname: Ueffing
authors.forename: Horst
authors.initials: H
authors.lastname: Zitzelsberger
authors.forename: Heinz
authors.initials: H
authors.lastname: Höfler
authors.forename: Axel
authors.initials: A
authors.lastname: Walch
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D017306:Annexin A2 / Q000096:biosynthesis*; D014408:Biomarkers, Tumor / Q000096:biosynthesis*; D003576:Electron Transport Complex IV / Q000096:biosynthesis*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D008840:Microfilament Proteins / Q000096:biosynthesis*; D009124:Muscle Proteins / Q000096:biosynthesis*; D009361:Neoplasm Invasiveness; D011379:Prognosis; D040901:Proteomics / Q000379:methods; D009418:S100 Proteins / Q000096:biosynthesis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D000099248:S100 Calcium Binding Protein A10
chemical_list: D017306:Annexin A2; D014408:Biomarkers, Tumor; D003576:Electron Transport Complex IV; D008840:Microfilament Proteins; D009124:Muscle Proteins; D009418:S100 Proteins; D000099248:S100 Calcium Binding Protein A10; C051615:transgelin