title: Detection of Listeria monocytogenes in ready-to-eat food by Step One real-time polymerase chain reaction.
abstract: The aim of this study was to follow contamination of ready-to-eat food with Listeria monocytogenes by using the Step One real time polymerase chain reaction (PCR). We used the PrepSEQ Rapid Spin Sample Preparation Kit for isolation of DNA and MicroSEQ® Listeria monocytogenes Detection Kit for the real-time PCR performance. In 30 samples of ready-to-eat milk and meat products without incubation we detected strains of Listeria monocytogenes in five samples (swabs). Internal positive control (IPC) was positive in all samples. Our results indicated that the real-time PCR assay developed in this study could sensitively detect Listeria monocytogenes in ready-to-eat food without incubation.
authors.affiliation: Department of Microbiology, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic.
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Pochop
authors.forename: Miroslava
authors.initials: M
authors.lastname: Kačániová
authors.forename: Lukáš
authors.initials: L
authors.lastname: Hleba
authors.forename: L'ubomír
authors.initials: L
authors.lastname: Lopasovský
authors.forename: Alica
authors.initials: A
authors.lastname: Bobková
authors.forename: Lucia
authors.initials: L
authors.lastname: Zeleňáková
authors.forename: Michal
authors.initials: M
authors.lastname: Stričík
mesh_terms: D000818:Animals; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D003611:Dairy Products / Q000382:microbiology*; D005516:Food Microbiology / Q000295:instrumentation / Q000379:methods*; D008089:Listeria monocytogenes / Q000302:isolation & purification*; D008460:Meat / Q000382:microbiology*; D011933:Reagent Kits, Diagnostic; D060888:Real-Time Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*; D012474:Salmon / Q000382:microbiology; D017747:Seafood / Q000382:microbiology; D012680:Sensitivity and Specificity; D012756:Sheep / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic