title: Liver proteome changes induced by a short-term high-fat sucrose diet in wistar rats.
abstract: OBJECTIVE
  The aim of this study was to gain insight into those proteins that might be involved in the early stages of liver fat accumulation as a consequence of a different fat versus simple sugar dietary intake.
  
  
  METHODS
  Forty-five male Wistar rats were randomly distributed into four dietary groups: a starch-rich control diet (CD; n = 10), a high-fat diet (n = 12), a high-sucrose diet (n = 11), and a high-fat sucrose diet (HFSD; n = 12) for 5 weeks. A comparative analysis by 2D-DIGE and LC-ESI-MS/MS was performed to characterize the liver protein expression profiles due to the three obesogenic diets.
  
  
  RESULTS
  Ten out of 17 proteins whose expression levels were altered by >1.25-fold were identified. Four proteins (Hspa8, Hspa9, Ca3, and Cat) were differentially expressed after the HFSD period compared to CD. The heat shock proteins (Hspa8 and Hspa9) resulted significantly downregulated in liver from rats fed HFSD versus CD (p < 0.05). The results were confirmed by Western blot.
  
  
  CONCLUSIONS
  This descriptive study might be useful for further studies aiming at understanding the mechanisms by which diets rich in both fat and sugar affect the initiation of hepatic steatosis.
authors.affiliation: Department of Nutrition, Food Science, Physiology and Toxicology, University of Navarra, Pamplona, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Bondia-Pons
authors.forename: Noemí
authors.initials: N
authors.lastname: Boqué
authors.forename: Laura
authors.initials: L
authors.lastname: Paternain
authors.forename: Enrique
authors.initials: E
authors.lastname: Santamaría
authors.forename: Joaquín
authors.initials: J
authors.lastname: Fernández
authors.forename: Javier
authors.initials: J
authors.lastname: Campión
authors.forename: Fermín
authors.initials: F
authors.lastname: Milagro
authors.forename: Fernando
authors.initials: F
authors.lastname: Corrales
authors.forename: J Alfredo
authors.initials: JA
authors.lastname: Martínez
mesh_terms: D000273:Adipose Tissue / Q000033:anatomy & histology / Q000187:drug effects; D000818:Animals; D001835:Body Weight / Q000187:drug effects / Q000502:physiology; D059305:Diet, High-Fat / Q000009:adverse effects; D004041:Dietary Fats / Q000494:pharmacology*; D019422:Dietary Sucrose / Q000494:pharmacology*; D008099:Liver / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D055442:Metabolome / Q000187:drug effects; D020543:Proteome / Q000032:analysis / Q000187:drug effects* / Q000378:metabolism; D040901:Proteomics; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D004041:Dietary Fats; D019422:Dietary Sucrose; D020543:Proteome