title: [Screening of three novel antimicrobial peptides with antifungal pathogens].
abstract: OBJECTIVE
  In order to discover novel antimicrobial peptides against important crop pathogens, we designed and screened a high capacity random peptide library and isolated a number of clones expressing peptides with antifungal activity. We selected 96 peptides from the library and synthesized their sequence, which were used to assay their activity against crop fungal pathogens.
  
  
  METHODS
  Using agar diffusion assay, these peptides were assayed for their activity against pathogens that cause cotton Fusarium wilt (Fusarium f. sp, vasinfecum), cotton red rot (Fusarium moniliforme), wheat spot blotch (Bipolaris sorokiniana) and potato early blight (Alternaria solani).
  
  
  RESULTS
  The three random peptides, A6, D4 and F10, showed the strongest activity against the above four crop fungal pathogens. Through Blastp analysis, we did not find they have homologous sequences with known antimicrobial peptides.
  
  
  CONCLUSIONS
  The novel antimicrobial peptides will provide gene resources for preventing important crop pathogens.
authors.affiliation: College of Life Sciences, Agricultural University of Hebei, Baoding 071001, China. lanjinping_mbb@126.com
authors.forename: Jinping
authors.initials: J
authors.lastname: Lan
authors.forename: Liyun
authors.initials: L
authors.lastname: Li
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.forename: Xianyun
authors.initials: X
authors.lastname: Wang
authors.forename: Lijuan
authors.initials: L
authors.lastname: Liu
authors.forename: Gouzhen
authors.initials: G
authors.lastname: Liu
authors.forename: Xiongying
authors.initials: X
authors.lastname: Cheng
mesh_terms: D001203:Ascomycota / Q000187:drug effects* / Q000254:growth & development; D004353:Drug Evaluation, Preclinical; D005659:Fungicides, Industrial / Q000737:chemistry / Q000494:pharmacology*; D005670:Fusarium / Q000187:drug effects* / Q000254:growth & development; D015394:Molecular Structure; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D010935:Plant Diseases / Q000382:microbiology*
chemical_list: D005659:Fungicides, Industrial; D010455:Peptides