title: Metabolism of oak leaf ellagitannins and urolithin production in beef cattle.
abstract: Oak leaves have a high concentration of ellagitannins. These phytochemicals can be beneficial or poisonous to animals. Beef cattle are often intoxicated by oak leaf consumption, particularly after suffering feed restriction. The severity of the poisoning has recently been associated with the ruminal microbiota, as different bacterial populations were found in animals that tolerated oak leaves and in those that showed clinical and pathological signs of toxicity. Intoxication has previously been linked to the production of phenolic metabolites, particularly catechol, phloroglucinol, and resorcinol. This suggested that the microbial metabolism of ellagitannins could also be associated with its tolerance or intoxication in different animals. Therefore, it is essential to understand the metabolism of ellagitannins in cattle. Here we show that ellagitannins are metabolized in the cattle rumen to urolithins. Different urolithins were detected in ruminal fluid, feces, urine, and plasma. Oak leaf ellagitannins declined as they were converted to urolithins, mainly isourolithin A and urolithin B, by the ruminal and fecal microbiota. Urolithin aglycons were observed in rumen and feces, and glucuronide and sulfate derivatives were detected in plasma and urine. Sulfate derivatives were the main metabolites detected in plasma, while glucuronide derivatives were the main ones in urine. The main urolithins produced in cattle were isourolithin A and urolithin B. This is a relevant difference from the monogastric mammals studied previously in which urolithin A was the main metabolite produced. Low molecular weight phenolics of the benzoic, phenylacetic, and phenylpropionic groups and metabolites such as catechol, resorcinol, and related compounds were also detected. There was a large variability in the kinetics of production of these metabolites in individual animals, although they produced similar metabolites in all cases. This large variability could be associated with the large variability in the rumen and intestine microbiota that has previously been observed. Further studies are needed to demonstrate if the efficiency in the metabolism of ellagitannins by the microbiota could explain the differences observed in susceptibility to intoxication by the different animals.
authors.affiliation: Research Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS-CSIC, P.O. Box 164, 30100 Campus de Espinardo, Murcia, Spain.
authors.forename: Rocío
authors.initials: R
authors.lastname: González-Barrio
authors.forename: Pilar
authors.initials: P
authors.lastname: Truchado
authors.forename: Rocío
authors.initials: R
authors.lastname: García-Villalba
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Hervás
authors.forename: Pilar
authors.initials: P
authors.lastname: Frutos
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Espín
authors.forename: Francisco A
authors.initials: FA
authors.lastname: Tomás-Barberán
mesh_terms: D000818:Animals; D001419:Bacteria / Q000378:metabolism; D002417:Cattle / Q000378:metabolism* / Q000382:microbiology; D002851:Chromatography, High Pressure Liquid; D003374:Coumarins / Q000097:blood / Q000378:metabolism* / Q000652:urine; D005243:Feces / Q000382:microbiology; D008401:Gas Chromatography-Mass Spectrometry; D047348:Hydrolyzable Tannins / Q000378:metabolism / Q000493:pharmacokinetics*; D008297:Male; D013058:Mass Spectrometry; D018515:Plant Leaves / Q000737:chemistry* / Q000633:toxicity; D029963:Quercus; D012417:Rumen / Q000378:metabolism / Q000382:microbiology
chemical_list: D003374:Coumarins; D047348:Hydrolyzable Tannins; C013515:ellagitannin; C026423:3,8-dihydroxy-6H-dibenzo(b,d)pyran-6-one