title: Diversity of a mesophilic lignocellulolytic microbial consortium which is useful for enhancement of biogas production.
abstract: A mesophilic lignocellulolytic microbial consortium BYND-5, established by successive subcultivation, was applied to enhance the biogas production. The degradation efficiency of BYND-5 for rice straw was more than 49.0 ± 1.8% after 7 days of cultivation at 30°C. Various organic compounds, including acetic acid, propionic acid, butyric acid and glycerin were detected during biodegradation. The diversity analysis of BYND-5 was conducted by ARDRA (Amplified ribosomal DNA restriction analysis) of the 16S rDNA clone library. Results indicated that bacterial groups represented in the clone library were the Firmicutes (5.96%), the Bacteroidetes (40.0%), Deferribacteres (8.94%), Proteobacteria (16.17%), Lentisphaerae (2.13%), Fibrobacteraceae (1.7%), and uncultured bacterium (25.1%). Additionally, the enhancement of biogas yield and methane content was directly related to the pretreatment with BYND-5. The microbial community identified herein is potential candidate consortium for the degradation of waste lignocellulose and enhancement of biogas production under the mesophilic temperature conditions.
authors.affiliation: College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, PR China. hekouyanlei@gmail.com
authors.forename: Lei
authors.initials: L
authors.lastname: Yan
authors.forename: Yamei
authors.initials: Y
authors.lastname: Gao
authors.forename: Yanjie
authors.initials: Y
authors.lastname: Wang
authors.forename: Quan
authors.initials: Q
authors.lastname: Liu
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Sun
authors.forename: Borui
authors.initials: B
authors.lastname: Fu
authors.forename: Xue
authors.initials: X
authors.lastname: Wen
authors.forename: Zongjun
authors.initials: Z
authors.lastname: Cui
authors.forename: Weidong
authors.initials: W
authors.lastname: Wang
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D001483:Base Sequence; D001673:Biodegradation, Environmental; D056804:Biofuels; D017931:DNA Primers; D005285:Fermentation; D008031:Lignin / Q000378:metabolism*; D010802:Phylogeny
chemical_list: D056804:Biofuels; D017931:DNA Primers; C036909:lignocellulose; D008031:Lignin