title: Overexpression of IgLON cell adhesion molecules changes proliferation and cell size of cortical astrocytes.
abstract: IgLON family is a subgroup of the immunoglobulin superfamily cell adhesion molecules and composed of limbic system-associated protein (LAMP), opioid binding cell adhesion molecule (OBCAM), neurotrimin (Ntm) and Kilon. In the present study, we investigated the overexpression of LAMP, OBCAM, Ntm and Kilon on the proliferation and cell size of type-1 astrocytes in vitro. Quantitative analysis using bromodeoxyuridine immunocytochemistry revealed that the expression of OBCAM had greater inhibitory effect on astrocytic proliferation as compared with LAMP, Ntm and Kilon ones. OBCAM overexpression increased the cell size of astrocytes as compared with the control. The treatment of FGF-2 had greater proliferative effect on OBCAM-transfected astrocytes as compared with the control. These results suggest that OBCAM is more potent regulator for controlling the proliferation and cell size of astrocytes as compared with other IgLON proteins possibly through FGF-2 receptor-mediated pathway.
authors.affiliation: Department of Applied Biology, Kyoto Institute of Technology, Kyoto, Japan.
authors.forename: Chiaki
authors.initials: C
authors.lastname: Sugimoto
authors.forename: Shoko
authors.initials: S
authors.lastname: Morita
authors.forename: Seiji
authors.initials: S
authors.lastname: Miyata
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology* / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000235:genetics* / Q000378:metabolism*; D049109:Cell Proliferation; D048429:Cell Size; D002540:Cerebral Cortex / Q000166:cytology*; D058851:GPI-Linked Proteins / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D051381:Rats; D017208:Rats, Wistar; D051497:Receptor, Fibroblast Growth Factor, Type 2 / Q000378:metabolism
chemical_list: D015815:Cell Adhesion Molecules; D058851:GPI-Linked Proteins; C489215:Opcml protein, rat; D051497:Receptor, Fibroblast Growth Factor, Type 2