title: Kinetics of aggregation of UV-irradiated glyceraldehyde-3-phosphate dehydrogenase from rabbit skeletal muscle. Effect of agents possessing chaperone-like activity.
abstract: An aggregation test system based on the aggregation of UV-irradiated glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from rabbit skeletal muscle has been proposed. On the basis of the measurements of the enzyme activity and differential scanning calorimetry data a conclusion has been made that UV radiation results in formation of damaged protein molecules with lower thermostability. It was shown that the order of aggregation rate for UV-irradiated GAPDH with respect to the protein was close to 2. This means that such a test system allows detecting the effect of various agents exclusively on the stage of aggregation of unfolded protein molecules. The influence of α-crystallin and 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) on aggregation of UV-irradiated GAPDH was studied. Despite the fact that HP-β-CD accelerates thermal aggregation of non-irradiated GAPDH, in the case of aggregation of UV-irradiated GAPDH HP-β-CD reveals a purely protective effect.
authors.affiliation: Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Olga I
authors.initials: OI
authors.lastname: Maloletkina
authors.forename: Kira A
authors.initials: KA
authors.lastname: Markossian
authors.forename: Natalia A
authors.initials: NA
authors.lastname: Chebotareva
authors.forename: Regina A
authors.initials: RA
authors.lastname: Asryants
authors.forename: Sergey Yu
authors.initials: SY
authors.lastname: Kleymenov
authors.forename: Nikolay B
authors.initials: NB
authors.lastname: Poliansky
authors.forename: Konstantin O
authors.initials: KO
authors.lastname: Muranov
authors.forename: Valentina F
authors.initials: VF
authors.lastname: Makeeva
authors.forename: Boris I
authors.initials: BI
authors.lastname: Kurganov
mesh_terms: D000073738:2-Hydroxypropyl-beta-cyclodextrin; D000818:Animals; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D018832:Molecular Chaperones / Q000737:chemistry; D018482:Muscle, Skeletal / Q000201:enzymology*; D011489:Protein Denaturation; D055550:Protein Stability; D011817:Rabbits; D013696:Temperature; D014466:Ultraviolet Rays; D038201:alpha-Crystallins / Q000737:chemistry; D047392:beta-Cyclodextrins / Q000737:chemistry
chemical_list: D018832:Molecular Chaperones; D038201:alpha-Crystallins; D047392:beta-Cyclodextrins; D000073738:2-Hydroxypropyl-beta-cyclodextrin; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases