title: Effects of silencing cyclooxygenase-2 expression via RNA interference             on the tumorigenicity of the SMMC-7721 human hepatocarcinoma cell line.
abstract: We constructed a vector carrying a shRNA sequence against cyclooxygenase-2             (COX-2) that was subsequently transfected into the human hepatocarcinoma cell             line SMMC‑7721. Furthermore, we established a COX-2-deficient stable cell line             and a model of tumor-shRNA transplantation in nude mice. Negative shRNA was used             as the control. The tumor volume in the experimental group was smaller compared             to that in the control group. Hematoxylin and eosin staining indicated that the             cells in the experimental group differentiated better than those in the control             group. The COX-2 mRNA level in the tumor tissues injected with SMMC-7721/COX-2i             was markedly downregulated compared to that in the tumor tissues injected with             SMMC-7721/negative shRNA. The inhibition rate reached 68.6%. Immunohistological             study showed a significantly strong COX-2 expression in the control group tumor             cells, whereas the experimental group exhibited moderate expression, indicating             the inhibition of COX-2 expression after transfection of cells with shRNA against             COX-2. Western blot analysis further proved the inhibition of COX-2 expression.             In conclusion, RNAi-mediated regulation of COX-2 expression could efficiently             inhibit liver-transplanted tumor growth in BALB/c nude mice.
authors.affiliation: Center of Clinical Laboratory, Zhongshan Hospital, Medical College of Xiamen University, Xiamen 361004, PR China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Liu
authors.forename: Li-Rong
authors.initials: LR
authors.lastname: Lin
authors.forename: Man-Li
authors.initials: ML
authors.lastname: Tong
authors.forename: Zuo-Gen
authors.initials: ZG
authors.lastname: Fu
authors.forename: Gui-Li
authors.initials: GL
authors.lastname: Liu
authors.forename: Wei-Hong
authors.initials: WH
authors.lastname: Zheng
authors.forename: Fan
authors.initials: F
authors.lastname: Liu
authors.forename: Dai-Wei
authors.initials: DW
authors.lastname: Zhang
authors.forename: Zhong-Ying
authors.initials: ZY
authors.lastname: Zhang
authors.forename: Tian-Ci
authors.initials: TC
authors.lastname: Yang
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology / Q000628:therapy*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D051546:Cyclooxygenase 2 / Q000235:genetics* / Q000378:metabolism*; D005822:Genetic Vectors; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology / Q000628:therapy*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D034622:RNA Interference; D012333:RNA, Messenger / Q000096:biosynthesis; D034741:RNA, Small Interfering; D023041:Xenograft Model Antitumor Assays
chemical_list: D012333:RNA, Messenger; D034741:RNA, Small Interfering; D051546:Cyclooxygenase 2