title: Differences in sexual development in inbred and outbred zebrafish (Danio rerio) and implications for chemical testing.
abstract: Outbred laboratory animal strains used in ecotoxicology are intended to represent wild populations. However, breeding history may vary considerably between strains, driving differences in genetic variation and phenotypes used for assessing effects of chemical exposure. We compared a range of phenotypic endpoints in zebrafish from four different "breeding treatments" comprising a Wild Indian Karyotype (WIK) zebrafish strain and a WIK/Wild strain with three levels of inbreeding (F(IT)=n, n+0.25, n+0.375) in a new Fish Sexual Development Test (FSDT). There were no differences between treatments in terms of egg viability, hatch success or fry survival. However, compared with WIKs, WIK/Wild hybrids were significantly larger in size, with more advanced gonadal (germ cell) development at the end of the test (63 days post fertilisation). Increasing the levels of inbreeding in the related WIK/Wild lines did not affect body size, but there was a significant male-bias (72%) in the most inbred line (F(IT)=n+0.375). Conversely, in the reference WIK strain there was a significant female-bias in the population (80% females). Overall, our results support the use of outbred zebrafish strains in the FSDT, where one of the core endpoints is sex ratio. Despite increased variance (and reduced statistical power) for some endpoints, WIK/Wild outbreds (F(IT)=n) met all acceptance criteria for controls in this test, whereas WIKs failed to comply with tolerance limits for sex ratio (30-70% females). Sexual development was also more advanced in WIK/Wild outbreds (cf. WIKs), providing greater scope for detection of developmental reproductive toxicity following chemical exposure.
authors.affiliation: AstraZeneca Safety, Health & Environment, Brixham Environmental Laboratory, Freshwater Quarry, Brixham, UK.
authors.forename: A Ross
authors.initials: AR
authors.lastname: Brown
authors.forename: Lisa K
authors.initials: LK
authors.lastname: Bickley
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Ryan
authors.forename: Gregory C
authors.initials: GC
authors.lastname: Paull
authors.forename: Patrick B
authors.initials: PB
authors.lastname: Hamilton
authors.forename: Stewart F
authors.initials: SF
authors.lastname: Owen
authors.forename: Alan D
authors.initials: AD
authors.lastname: Sharpe
authors.forename: Charles R
authors.initials: CR
authors.lastname: Tyler
mesh_terms: D000818:Animals; D049628:Body Size; D001947:Breeding; D005260:Female; D014644:Genetic Variation; D006066:Gonads / Q000254:growth & development; D007178:Inbreeding; D008297:Male; D046468:Sexual Development / Q000502:physiology*; D013045:Species Specificity; D013997:Time Factors; D018675:Toxicity Tests; D015027:Zebrafish / Q000235:genetics / Q000502:physiology*