title: Impaired cell functions of hepatocytes incubated with plasma of septic patients.
abstract: OBJECTIVE
  The development of liver failure is a major problem in septic patients. In this prospective clinical experimental study the hepatotoxicity of plasma from septic and non-septic patients was tested.
  
  
  METHODS
  The basic test components consist of human liver cells (HepG2/C3A) used in a standardized microtiter plate assay. After incubation with patient's plasma viability of cells (XTT-test), the cytochrome 1A2 activity and synthesis of micro albumin were measured. Subjects (28) enrolled comprise the septic shock group (SSG, n=10), the non-septic group (NSG, n=5) and the healthy volunteers group (HVG, n=13).
  
  
  RESULTS
  The 28-day mortality was 30% in the SSG. The APACHE II-, SOFA-, and SAPS-scores and the values of bilirubin and prothrombin time as INR were significantly higher in the SSG than in the NSG. The cytochrome 1A2 activity and the release of albumin were significantly reduced in HepG2/C3A cells incubated with plasma of the SSG (p<0.05). The cytochrome 1A2 activities were higher in survivors compared to non-survivors at the time point 0 and were increasing in survivors and decreasing in non-survivors within 54 h in the SSG. In the SSG there was a significant decrease in IL-10 and IL-8 between inclusion and 54 h. Values of IL-6, TNF alpha and IL-10 were significantly lower in the NSG compared with the values of the SSG at inclusion and after 54 h.
  
  
  CONCLUSIONS
  The plasma of patients with septic shock impaired cellular functions of HepG2/C3A cells.
authors.affiliation: Department of Anaesthesiology and Intensive Care Medicine, University of Rostock, Schillingallee 35, 18055, Rostock, Germany. martin.sauer@uni-rostock.de
authors.forename: Martin
authors.initials: M
authors.lastname: Sauer
authors.forename: Cristof
authors.initials: C
authors.lastname: Haubner
authors.forename: Thomas
authors.initials: T
authors.lastname: Mencke
authors.forename: Gabriele
authors.initials: G
authors.lastname: Nöldge-Schomburg
authors.forename: Steffen
authors.initials: S
authors.lastname: Mitzner
authors.forename: Jens
authors.initials: J
authors.lastname: Altrichter
authors.forename: Jan
authors.initials: J
authors.lastname: Stange
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000418:Albumins / Q000378:metabolism; D001663:Bilirubin / Q000097:blood; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D019388:Cytochrome P-450 CYP1A2 / Q000378:metabolism; D016207:Cytokines / Q000097:blood; D005260:Female; D022781:Hepatocytes / Q000378:metabolism*; D006801:Humans; D017093:Liver Failure / Q000097:blood / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D018805:Sepsis / Q000097:blood / Q000378:metabolism*
chemical_list: D000418:Albumins; D016207:Cytokines; C504573:CYP1A2 protein, human; D019388:Cytochrome P-450 CYP1A2; D001663:Bilirubin