title: Tostadin, a novel antibacterial peptide from an antagonistic microorganism Brevibacillus brevis XDH.
abstract: A novel small antibacterial peptide was obtained from the liquid culture of Brevibacillus brevis XDH, which is a broad-spectrum antagonistic bacterium isolated from the soil of Mountain Tai, China. This peptide was purified from the fermentation medium of strain XDH via ammonium sulfate precipitation, cation exchange chromatography, and reversed-phase high-performance liquid chromatography (HPLC), successively. The structure of the active linear peptide was elucidated using mass spectra (MS) and nuclear magnetic resonance (NMR) analyses that consisted of nine amino acids. This peptide was easily soluble in water, thermally stable and strongly inhibited the growth of Escherichia coli and Staphylococcus aureus in vitro. The present data support the identification of a novel antibacterial peptide, which was named Tostadin.
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Song
authors.forename: Qingxin
authors.initials: Q
authors.lastname: Liu
authors.forename: Hui
authors.initials: H
authors.lastname: Guo
authors.forename: Ruicheng
authors.initials: R
authors.lastname: Ju
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Zhao
authors.forename: Jinyu
authors.initials: J
authors.lastname: Li
authors.forename: Xunli
authors.initials: X
authors.lastname: Liu
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000302:isolation & purification / Q000494:pharmacology*; D058675:Brevibacillus / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003470:Culture Media; D005285:Fermentation; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D003470:Culture Media; C573662:tostadin peptide, Brevibacillus brevis