title: Differential expression profile and genetic variants of microRNAs sequences in breast cancer patients.
abstract: The technology available for cancer diagnosis and prognosis is not yet satisfactory at the molecular level, and requires further improvements. Micro RNAs (miRNAs) have been recently reported as useful biomarkers in diseases including cancer. We performed a miRNA expression profiling study using peripheral blood from breast cancer patients to detect and identify characteristic patterns. A total of 100 breast cancer patients and 89 healthy patients were recruited for miRNA genotyping and expression profiling. We found that hs-miR-196a2 in premenopausal patients, and hs-miR-499, hs-miR-146a and hs-miR-196a2 in postmenopausal patients, may discriminate breast cancer patients from healthy individuals. In addition, we found a significant association between two microRNA polymorphisms (hs-miR-196a2 and hs-miR-499) and breast cancer risk. However, no significant association between the hs-miR-146a gene and breast cancer risk was found. In summary, the study demonstrates that peripheral blood miRNAs and their expression and genotypic profiles can be developed as biomarkers for early diagnosis and prognosis of breast cancer.
authors.affiliation: Molecular Cancer Biology Research Lab (MCBRL), Department of Food Sciences and Nutrition, College of Food and Agricultural Sciences, King Saud University, Riyadh, Saudi Arabia. alialshatwi@gmail.com
authors.forename: Ali A
authors.initials: AA
authors.lastname: Alshatwi
authors.forename: Gowhar
authors.initials: G
authors.lastname: Shafi
authors.forename: Tarique N
authors.initials: TN
authors.lastname: Hasan
authors.forename: Naveed Ahmed
authors.initials: NA
authors.lastname: Syed
authors.forename: Amal A
authors.initials: AA
authors.lastname: Al-Hazzani
authors.forename: Mohammed A
authors.initials: MA
authors.lastname: Alsaif
authors.forename: Abdulaziz A
authors.initials: AA
authors.lastname: Alsaif
mesh_terms: D000328:Adult; D000483:Alleles; D001483:Base Sequence; D001943:Breast Neoplasms / Q000097:blood / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D014644:Genetic Variation; D060005:Genotyping Techniques; D006801:Humans; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D009367:Neoplasm Staging; D016017:Odds Ratio; D017698:Postmenopause / Q000097:blood / Q000235:genetics
chemical_list: D035683:MicroRNAs