title: [Expression of a protein elicitor pebC1 from Botrytis cinerea in Pichia pastoris].
abstract: In order to express PebC1 in Pichia pastoris, the pebC1 sequence was amplified from genome Botrytis cinerea BC-4-2-2-1 by PCR and subcloned into the Pichua pastoris expression vector pPIC9K to generate pPIC9K-pebC1. The recombinant plasmid was linearized by Bgl II and transformed into Pichia pastoris GS115 by electroporation. Recombinant Pichia pastoris GS115/pPIC9K-pebC1 was screened by MD and G418-YPD plates and further confirmed by PCR. The protein expression was induced by methanol and analyzed by SDS-PAGE. SDS-PAGE analysis showed a special band about 39 kDa and western blotting indicated a good antigenicity of the expressed protein. Bioassay results showed that the recombinant protein PebC1 can induce resistance to gray mould disease of cucumber and Arabidopsi thaliana.
authors.affiliation: Key Laboratory of Integrated Pest Management in Crops, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Yunhua
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiufen
authors.initials: X
authors.lastname: Yang
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Liu
authors.forename: Shanjiang
authors.initials: S
authors.lastname: Yu
authors.forename: Dewen
authors.initials: D
authors.lastname: Qiu
mesh_terms: D020171:Botrytis / Q000737:chemistry*; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology; D010843:Pichia / Q000235:genetics* / Q000378:metabolism; D010935:Plant Diseases / Q000517:prevention & control; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology
chemical_list: D005656:Fungal Proteins; D011994:Recombinant Proteins