title: De novo intestine-specific transcriptome of the brown planthopper Nilaparvata lugens revealed potential functions in digestion, detoxification and immune response.
abstract: The brown planthopper (Nilaparvata lugens, BPH) is the most serious rice plant pests in Asia. In this study, we performed transcriptome-wide analysis on BPH intestine. We obtained more than 26 million sequencing reads that were then assembled into 53,553 unigenes with a mean size of 388 bp. Based on similarity search with the nucleotide sequences available at NCBI, BPH intestine-specific transcriptome analysis identified 21,405 sequences. Assembled sequences were annotated with gene description, gene ontology and clusters of orthologous group terms. The digestion-, defense- and xenobiotic metabolism-related genes were abundantly detected in the transcripts from BPH intestine. Many novel genes including 33 digestion-related genes, 25 immune responsive genes and 27 detoxification-related genes are first reported here. We investigated the gene expression patterns at the transcript levels in different tissues by quantitative real-time PCR analysis, which revealed that some genes had intestine-specific expression, implicating their potential significance for BPH management.
authors.affiliation: State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou 310028, China.
authors.forename: Yan-Yuan
authors.initials: YY
authors.lastname: Bao
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Wen-Juan
authors.initials: WJ
authors.lastname: Wu
authors.forename: Dong
authors.initials: D
authors.lastname: Zhao
authors.forename: Jian
authors.initials: J
authors.lastname: Xue
authors.forename: Bao-Qin
authors.initials: BQ
authors.lastname: Zhang
authors.forename: Zhi-Cheng
authors.initials: ZC
authors.lastname: Shen
authors.forename: Chuan-Xi
authors.initials: CX
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001208:Asia; D030541:Databases, Genetic; D004063:Digestion / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation; D015723:Gene Library; D017344:Genes, Insect; D006430:Hemiptera / Q000235:genetics* / Q000378:metabolism; D007109:Immunity / Q000235:genetics; D007413:Intestinal Mucosa / Q000378:metabolism*; D005810:Multigene Family; D012275:Oryza; D010572:Pest Control, Biological / Q000379:methods; D017423:Sequence Analysis, RNA; D059467:Transcriptome