title: A 6-hydroxydopamine in vivo model of Parkinson's disease.
abstract: Animal models of Parkinson's disease are essential to explore pathophysiological hypotheses and to test new treatment options, including neurotrophic factors. Catecholaminergic neurotoxins used to generate such models are 6-hydroxydopamine and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine. These neurotoxins predominantly kill dopaminergic neurons through oxidative damage and mitochondrial failure, although 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine fails to induce a significant dopaminergic neurodegeneration in rats. The present chapter describes a protocol for the 6-hydroxydopamine rat model based on stereotaxic injection performed only unilaterally, which mimics an early-to-mid stage of the disease.
authors.affiliation: Department of Pharmacology and Anesthesiology, University of Padova, Padova, Italy. giulia.mercanti@unipd.it
authors.forename: Giulia
authors.initials: G
authors.lastname: Mercanti
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Bazzu
authors.forename: Pietro
authors.initials: P
authors.lastname: Giusti
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D059290:Dopaminergic Neurons / Q000187:drug effects*; D016627:Oxidopamine / Q000008:administration & dosage / Q000633:toxicity*; D010302:Parkinson Disease, Secondary / Q000139:chemically induced* / Q000601:surgery*; D051381:Rats
chemical_list: D016627:Oxidopamine