title: Near-infrared fluorescence imaging of mouse myocardial microvascular endothelium using Cy5.5-lectin conjugate.
abstract: Cy5.5-lectin, a non-toxic conjugate, combines the benefits of near-infrared (NIR) imaging, such as significant reduction of background fluorescence and increased tissue depth penetration, with its affinity for vascular endothelial cells. When compared to endothelial staining methods using FITC-lectin and ICAM2 antibodies, Cy5.5-lectin was confirmed to specifically bind endothelial cells and produce a fluorescence signal both in real-time and post-infusion. Ex-vivo experiments with isolated hearts demonstrated that binding was limited to perfused areas of the myocardium. With mouse in-vivo tail-vein injections, other organs such as the liver, spleen, and kidney were also stained and yielded similar quality images of the heart.
authors.affiliation: University of Manitoba, Department of Oral Biology, Winnipeg, MB, R3T 2N2, Canada.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Nguyen
authors.forename: Saro
authors.initials: S
authors.lastname: Bascaramurty
authors.forename: Bozena
authors.initials: B
authors.lastname: Kuzio
authors.forename: Lori
authors.initials: L
authors.lastname: Gregorash
authors.forename: Valery
authors.initials: V
authors.lastname: Kupriyanov
authors.forename: Olga
authors.initials: O
authors.lastname: Jilkina
mesh_terms: D000818:Animals; D002232:Carbocyanines / Q000378:metabolism*; D003331:Coronary Vessels / Q000166:cytology*; D004730:Endothelium, Vascular / Q000166:cytology* / Q000378:metabolism*; D005260:Female; D005456:Fluorescent Dyes / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D055806:Microvessels / Q000166:cytology*; D061848:Optical Imaging / Q000379:methods*; D010477:Perfusion; D037121:Plant Lectins / Q000378:metabolism*
chemical_list: C098793:CY5.5 cyanine dye; D002232:Carbocyanines; D005456:Fluorescent Dyes; D037121:Plant Lectins; C034981:tomato lectin