title: Serological studies on Corynebacterium pseudotuberculosis infections in goats in Baden-Wuerttemberg (Germany) and seroreactions on antigens used for newly developed enzyme-linked immunosorbent assays (ELISA).
abstract: In the present study, comprehensive data on the seroprevalence of Corynebacterium (C.) pseudotuberculosis infections in goats are presented for Baden-Wuerttemberg, a Federal State of Germany, for the first time. As a prerequisite, ELISAs based on a recombinant phospholipase D (rPLD) and whole cell antigens (WCA) were designed and validated yielding sensitivity values of 81% and 97% and specificity values of 98% and 99%, respectively. Immunisation trials in goats demonstrated a significant production of antibodies to rPLD but an evidently lower antibody production to WCA as determined in the corresponding ELISA. Moreover, immunisation with rPLD resulted in the formation of antibodies, which were also detected in the WCA ELISA. In contrast, this phenomenon was not observed with the rPLD ELISA after immunisation with WCA. Implementation of the rPLD and WCA ELISAs in a broad-based seroprevalence study in Baden-Wuerttemberg revealed positive reactions in both ELISAs in 13.2% of the 1771 goat sera tested. In 53.7% of 121 herds of which five or more animals were tested per herd there was at least one animal that showed a positive reaction in both tests.
authors.affiliation: Chemisches und Veterinäruntersuchungsamt Stuttgart. reinhard.sting@cvuas.bwl.de
authors.forename: Reinhard
authors.initials: R
authors.lastname: Sting
authors.forename: Brigitta
authors.initials: B
authors.lastname: Wagner
authors.forename: Asiye
authors.initials: A
authors.lastname: Sari-Turan
authors.forename: Marion
authors.initials: M
authors.lastname: Stermann
authors.forename: Markus
authors.initials: M
authors.lastname: Reule
authors.forename: Martin
authors.initials: M
authors.lastname: Eichner
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Beyer
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology; D003354:Corynebacterium Infections / Q000097:blood / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*; D016925:Corynebacterium pseudotuberculosis; D004797:Enzyme-Linked Immunosorbent Assay; D005858:Germany / Q000453:epidemiology; D015511:Goat Diseases / Q000097:blood / Q000175:diagnosis* / Q000453:epidemiology; D006041:Goats; D010739:Phospholipase D / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D015203:Reproducibility of Results; D016036:Seroepidemiologic Studies; D014614:Vaccines, Synthetic / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; D010739:Phospholipase D