title: Multiplexed suspension array platform for high-throughput protein assays.
abstract: A multiplexed suspension array platform, based on SU8 disks patterned with machine-readable binary identification codes is presented. Multiple probe molecules, each attached to individual disks with different unique codes, provide multiplexed detection of targets in a small sample volume. The experimental system consists of a microfluidic chamber for arraying the particles in a manner suitable for high throughput imaging using a simple fluorescent microscope, together with custom software for automated code readout and analysis of assay response. The platform is demonstrated with a multiplexed antibody assay targeting 3 different human inflammatory cytokines. The suitability of the platform for other bio-analytical applications is discussed.
authors.affiliation: School of Electronics and Computer Science, Highfield, Southampton, UK. sam.birtwell@gilltechnology.com
authors.forename: S W
authors.initials: SW
authors.lastname: Birtwell
authors.forename: G R
authors.initials: GR
authors.lastname: Broder
authors.forename: P L
authors.initials: PL
authors.lastname: Roach
authors.forename: H
authors.initials: H
authors.lastname: Morgan
mesh_terms: D055808:Drug Discovery; D004867:Equipment Design; D057166:High-Throughput Screening Assays / Q000295:instrumentation*; D006801:Humans; D040081:Protein Array Analysis / Q000295:instrumentation*; D013535:Suspensions
chemical_list: D013535:Suspensions