title: Surface modification of titanium by hydrothermal treatment in Mg-containing solution and early osteoblast responses.
abstract: Surface modification on titanium was carried out in order to improve its bioactivity. Pure titanium was hydrothermally treated in distilled water and 0.1 M MgCl(2) solutions at 200°C for 24 h. Surface morphology, roughness, wettability and chemical composition were characterized before and after treatment. Bovine serum albumin was used as model to study protein adsorption. MC3T3-E1 cells were cultured and initial cell attachment, morphology, proliferation were evaluated. After hydrothermal treatment, nano-sized precipitations were observed and samples showed superhydrophilicity. Magnesium (Mg) was immobilized into titanium surface by hydrothermal treatment. Protein adsorption was significantly increased on Mg-containing samples. Cell attachment was improved and cell spreading was enhanced on Mg-containing samples compared with untreated or those treated in distilled water. Increased early cellular attachment on the MgTi surface resulted in subsequent increase of number of proliferated cells. Hydrothermal treatment in MgCl(2) solution was expected to be an effective method to fabricate titanium implant with good bioactivity.
authors.affiliation: Department of Biomaterials, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, Japan.
authors.forename: Xingling
authors.initials: X
authors.lastname: Shi
authors.forename: Masaharu
authors.initials: M
authors.lastname: Nakagawa
authors.forename: Giichiro
authors.initials: G
authors.lastname: Kawachi
authors.forename: Lingli
authors.initials: L
authors.lastname: Xu
authors.forename: Kunio
authors.initials: K
authors.lastname: Ishikawa
mesh_terms: D000327:Adsorption / Q000187:drug effects; D000818:Animals; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D008274:Magnesium / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects* / Q000502:physiology; D012710:Serum Albumin, Bovine / Q000493:pharmacokinetics; D012996:Solutions / Q000737:chemistry / Q000494:pharmacology; D013499:Surface Properties; D013696:Temperature; D014025:Titanium / Q000737:chemistry*; D014867:Water / Q000494:pharmacology*; D017155:Wettability
chemical_list: D012996:Solutions; D014867:Water; D012710:Serum Albumin, Bovine; D014025:Titanium; D008274:Magnesium