title: Synthetic Coprisin analog peptide, D-CopA3 has antimicrobial activity and pro-apoptotic effects in human leukemia cells.
abstract: Recently, we reported that the synthetic Coprisin analog peptide 9-mer dimer CopA3 (consisted of all-L amino acid sequence) was designed based on a defensin-like peptide, Coprisin isolated from Copris tripartitus. The 9-mer dimer CopA3 (L-CopA3) had antibacterial activity and induced apoptosis in human leukemia cells via a caspase-independent pathway. In this study, all of amino acid sequences of L-CopA3 were modified to all D-form amino acids (D-CopA3) to develop a more effective antimicrobial peptide. We investigated whether D-CopA3 had antimicrobial activities against pathogenic microorganisms and proapoptotic effects in human leukemia cells (U937, Jurkat, and AML-2). The synthetic peptide D-CopA3 had antimicrobial activities against various pathogenic bacteria and yeast fungus with MIC values in the 4~64 microM range. Moreover, D-CopA3 caused cell growth inhibition, and increased the chromosomal DNA fragmentation and the expression of inflammatory cytokines, TNF-alpha and IL1-beta, transcripts in human leukemia cells. The all-D amino acid peptide D-CopA3 proved as effective as the L-CopA3 reported previously. These results provide the basis for developing D-CopA3 as a new antibiotic peptide.
authors.affiliation: Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Suwon 441-853, South Korea.
authors.forename: Soon-ja
authors.initials: SJ
authors.lastname: Kim
authors.forename: In-Woo
authors.initials: IW
authors.lastname: Kim
authors.forename: Yong-Nam
authors.initials: YN
authors.lastname: Kwon
authors.forename: Eun-Young
authors.initials: EY
authors.lastname: Yun
authors.forename: Jae-Sam
authors.initials: JS
authors.lastname: Hwang
mesh_terms: D023181:Antimicrobial Cationic Peptides / Q000138:chemical synthesis* / Q000235:genetics / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000494:pharmacology*; D017209:Apoptosis; D001419:Bacteria / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D053938:DNA Fragmentation; D005658:Fungi / Q000187:drug effects; D006801:Humans; D019476:Insect Proteins / Q000138:chemical synthesis* / Q000235:genetics / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests
chemical_list: D023181:Antimicrobial Cationic Peptides; D000970:Antineoplastic Agents; D019476:Insect Proteins; C570442:coprisin peptide, Copris tripartitus