title: Spectroscopic selection of distance measurements in a protein dimer with mixed nitroxide and Gd3+ spin labels.
abstract: The pulse DEER (Double Electron-Electron Resonance) technique is frequently applied for measuring nanometer distances between specific sites in biological macromolecules. In this work we extend the applicability of this method to high field distance measurements in a protein assembly with mixed spin labels, i.e. a nitroxide spin label and a Gd(3+) tag. We demonstrate the possibility of spectroscopic selection of distance distributions between two nitroxide spin labels, a nitroxide spin label and a Gd(3+) ion, and two Gd(3+) ions. Gd(3+)-nitroxide DEER measurements possess high potential for W-band long range distance measurements (6 nm) by combining high sensitivity with ease of data analysis, subject to some instrumental improvements.
authors.affiliation: Department of Chemical Physics, Weizmann Institute of Science, Rehovot, Israel.
authors.forename: Ilia
authors.initials: I
authors.lastname: Kaminker
authors.forename: Hiromasa
authors.initials: H
authors.lastname: Yagi
authors.forename: Thomas
authors.initials: T
authors.lastname: Huber
authors.forename: Akiva
authors.initials: A
authors.lastname: Feintuch
authors.forename: Gottfried
authors.initials: G
authors.lastname: Otting
authors.forename: Daniella
authors.initials: D
authors.lastname: Goldfarb
mesh_terms: D019281:Dimerization; D004578:Electron Spin Resonance Spectroscopy; D005682:Gadolinium / Q000737:chemistry*; D006360:Heat-Shock Proteins / Q000737:chemistry*; D008958:Models, Molecular; D015394:Molecular Structure; D009589:Nitrogen Oxides / Q000737:chemistry*; D013113:Spin Labels
chemical_list: D006360:Heat-Shock Proteins; D009589:Nitrogen Oxides; D013113:Spin Labels; D005682:Gadolinium