title: Isolation and characterization of Bacillus sp. producing broad-spectrum antibiotics against human and plant pathogenic fungi.
abstract: A strain of bacterium producing antifungal antibiotic was isolated and identification of the strain was attempted. We could identify the bacterium as being a Bacillus sp., based on morphological observation, physiological characteristics, and 16S rDNA sequence analysis, thus leading us to designate the strain as Bacillus sp. AH-E-1. The strain showed potent antibiotic activity against phytopathogenic and human pathogenic fungi by inducing mycelial distortion and swelling and inhibiting spore germination. The antibiotic metabolite produced by the strain demonstrated excellent thermal and pH (2-11) stability, but was labile to autoclaving. From these results, we could find a broader antifungal activity of Bacillus genus. Isolation and characterization of the active agent produced by the strain are under progress.
authors.affiliation: College of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.
authors.forename: Na
authors.initials: N
authors.lastname: Chen
authors.forename: Min
authors.initials: M
authors.lastname: Jin
authors.forename: Hong-mei
authors.initials: HM
authors.lastname: Qu
authors.forename: Zhi-qiang
authors.initials: ZQ
authors.lastname: Chen
authors.forename: Zhao-li
authors.initials: ZL
authors.lastname: Chen
authors.forename: Zhi-gang
authors.initials: ZG
authors.lastname: Qiu
authors.forename: Xin-wei
authors.initials: XW
authors.lastname: Wang
authors.forename: Jun-wen
authors.initials: JW
authors.lastname: Li
mesh_terms: D000935:Antifungal Agents / Q000378:metabolism*; D001407:Bacillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005658:Fungi / Q000187:drug effects* / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D009181:Mycoses / Q000382:microbiology; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology; D010944:Plants; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D000935:Antifungal Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S