title: Rhodopseudomonas pentothenatexigens sp. nov. and Rhodopseudomonas thermotolerans sp. nov., isolated from paddy soils.
abstract: Two strains (JA575(T) and JA576(T)) of orange- to pink-pigmented, rod-shaped, motile and budding phototrophic bacteria were isolated from paddy soils. Both strains contained bacteriochlorophyll a and carotenoids of spirilloxanthin series. Both strains had C(18 : 1)ω7c as the major cellular fatty acid, ubiquinone-10 (Q(10)) as the main quinone, and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine as polar lipids. Phylogenetic analysis on the basis of 16S rRNA gene sequences showed that both strains clustered with species of the genus Rhodopseudomonas in the class Alphaproteobacteria. Strains JA575(T) and JA576(T) were genotypically (<35 % DNA-DNA relatedness) and phenotypically distinct from each other. Further, both strains showed less than 48 % DNA-DNA relatedness with the type strains of all recognized species of the genus Rhodopseudomonas. The molecular evidence is supported by phenotypic evidence. It is proposed that strains JA575(T) and JA576(T) be classified as representing two novel species of the genus Rhodopseudomonas with the species names Rhodopseudomonas pentothenatexigens sp. nov. and Rhodopseudomonas thermotolerans sp. nov., respectively. The type strains of the proposed novel species are JA575(T) (= NBRC 108862(T) = KCTC15143(T)) and JA576(T) (= NBRC 108863(T) = KCTC 15144(T)), respectively.
authors.affiliation: Bacterial Discovery Laboratory, Centre for Environment, IST, JNT University Hyderabad, Kukatpally, Hyderabad 500 085, India.
authors.forename: B Vinay
authors.initials: BV
authors.lastname: Kumar
authors.affiliation: Bacterial Discovery Laboratory, Centre for Environment, IST, JNT University Hyderabad, Kukatpally, Hyderabad 500 085, India.
authors.forename: E V V
authors.initials: EVV
authors.lastname: Ramprasad
authors.affiliation: Bacterial Discovery Laboratory, Centre for Environment, IST, JNT University Hyderabad, Kukatpally, Hyderabad 500 085, India.
authors.forename: Ch
authors.initials: C
authors.lastname: Sasikala
authors.affiliation: Department of Plant Sciences, School of Life Sciences, University of Hyderabad, PO Central University, Hyderabad 500 046, India.
authors.forename: Ch V
authors.initials: CV
authors.lastname: Ramana
mesh_terms: D015373:Bacterial Typing Techniques; D025541:Bacteriochlorophyll A / Q000032:analysis; D001482:Base Composition; D002338:Carotenoids / Q000032:analysis; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology; D007194:India; D008969:Molecular Sequence Data; D012275:Oryza; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012241:Rhodopseudomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014451:Ubiquinone / Q000032:analysis
chemical_list: D025541:Bacteriochlorophyll A; D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; D002338:Carotenoids; C025204:Ubiquinone Q2