title: Nanoscale E-cadherin ligand patterns show threshold size for cellular adhesion and adherence junction formation.
abstract: The role of ligand spatial distribution on the formation of cadherin mediated cell-cell contacts is studied utilizing nanopatterns of E-cadherin ligands. Protein patches ranging in size from 100 to 800 nm prepared by colloidal lithography critically influence adhesion, spreading, and formation of adherence junctions in epithelial cells. Cells at 100 nm patterns show poor adhesion, while larger pattern sizes show good adhesion, significant spreading, and defined cortical actin. We estimate a threshold of 0.03 μm(2) for epithelial cellular attachment via E-Cadherin.
authors.affiliation: Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Denmark.
authors.forename: Stine H
authors.initials: SH
authors.lastname: Kristensen
authors.forename: Gitte A
authors.initials: GA
authors.lastname: Pedersen
authors.forename: Lene N
authors.initials: LN
authors.lastname: Nejsum
authors.forename: Duncan S
authors.initials: DS
authors.lastname: Sutherland
mesh_terms: D022005:Adherens Junctions; D000818:Animals; D015820:Cadherins / Q000737:chemistry*; D002448:Cell Adhesion; D003102:Colloids / Q000737:chemistry; D004285:Dogs; D004847:Epithelial Cells / Q000737:chemistry / Q000166:cytology*; D006046:Gold / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000737:chemistry; D008024:Ligands; D053768:Metal Nanoparticles / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D010316:Particle Size; D012822:Silicon Dioxide / Q000737:chemistry; D013499:Surface Properties
chemical_list: D015820:Cadherins; D003102:Colloids; D008024:Ligands; D049452:Green Fluorescent Proteins; D006046:Gold; D012822:Silicon Dioxide