title: Background current reduction and biobarcode amplification for label-free, highly sensitive electrochemical detection of pathogenic DNA.
abstract: By using exonuclease I and biobarcode nanoparticles, we describe a novel background current reduction strategy for amplified electrochemical detection of uropathogen specific sequences at ultralow concentrations.
authors.affiliation: Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, P. R. China.
authors.forename: Jin
authors.initials: J
authors.lastname: Xu
authors.forename: Bingying
authors.initials: B
authors.lastname: Jiang
authors.forename: Jiao
authors.initials: J
authors.lastname: Su
authors.forename: Yun
authors.initials: Y
authors.lastname: Xiang
authors.forename: Ruo
authors.initials: R
authors.lastname: Yuan
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Chai
mesh_terms: D020137:Base Pair Mismatch; D020029:Base Pairing; D015374:Biosensing Techniques; D004247:DNA; D058893:DNA Barcoding, Taxonomic / Q000295:instrumentation / Q000379:methods*; D015342:DNA Probes / Q000737:chemistry; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics*; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods; D004566:Electrodes; D004926:Escherichia coli / Q000235:genetics* / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000382:microbiology; D005090:Exodeoxyribonucleases / Q000378:metabolism; D006046:Gold / Q000737:chemistry; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D017975:Ruthenium Compounds / Q000737:chemistry; D012680:Sensitivity and Specificity; D014552:Urinary Tract Infections / Q000382:microbiology
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial; D017975:Ruthenium Compounds; D006046:Gold; D004247:DNA; D005090:Exodeoxyribonucleases; C028450:exodeoxyribonuclease I