title: Isolation, identification and characterization of Bacillus amyloliquefaciens BZ-6, a bacterial isolate for enhancing oil recovery from oily sludge.
abstract: Over 100 biosurfactant-producing microorganisms were isolated from oily sludge and petroleum-contaminated soil from Shengli oil field in north China. Sixteen of the bacterial isolates produced biosurfactants and reduced the surface tension of the growth medium from 71 to <30 mN m(-1) after 72 h of growth. These bacteria were used to treat oily sludge and the recovery efficiencies of oil from oily sludge were determined. The oil recovery efficiencies of different isolates ranged from 39% to 88%. Bacterial isolate BZ-6 was found to be the most efficient strain and the three phases (oil, water and sediment) were separated automatically after the sludge was treated with the culture medium of BZ-6. Based on morphological, physiological characteristics and molecular identification, isolate BZ-6 was identified as Bacillus amyloliquefaciens. The biosurfactant produced by isolate BZ-6 was purified and analyzed by high performance liquid chromatography-electrospray ionization tandem mass spectrometry. There were four ion peaks representing four different fengycin A homologues.
authors.affiliation: Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China.
authors.forename: Wuxing
authors.initials: W
authors.lastname: Liu
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Wang
authors.forename: Longhua
authors.initials: L
authors.lastname: Wu
authors.forename: Mengfang
authors.initials: M
authors.lastname: Chen
authors.forename: Chen
authors.initials: C
authors.lastname: Tu
authors.forename: Yongming
authors.initials: Y
authors.lastname: Luo
authors.forename: Peter
authors.initials: P
authors.lastname: Christie
mesh_terms: D001407:Bacillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D001419:Bacteria / Q000302:isolation & purification / Q000378:metabolism; D002681:China; D002851:Chromatography, High Pressure Liquid; D027081:DNA Gyrase / Q000235:genetics; D052918:Environmental Restoration and Remediation; D055666:Lipopeptides / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D010578:Petroleum / Q000378:metabolism / Q000382:microbiology; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012989:Soil Pollutants / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization; D013501:Surface-Active Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D053719:Tandem Mass Spectrometry
chemical_list: D055666:Lipopeptides; D010578:Petroleum; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D013501:Surface-Active Agents; C049972:fengycin; D027081:DNA Gyrase