title: Methods for iPS cell generation for basic research and clinical applications.
abstract: The induction of pluripotency can be achieved by forced expression of defined factors in somatic cells. The established cells, termed induced pluripotent stem (iPS) cells, have pluripotency and an infinite capacity for self-renewal in common with embryonic stem (ES) cells. Patient-specific iPS cells could be a useful source for drug discovery and cell transplantation therapies; however, the original method for iPS cell generation had several issues that were obstacles to their clinical application. Recent studies have brought about various improvements for iPS cell generation and uncovered several characteristics of iPS cells. Here we summarize the current status of iPS cell studies, with a focus on the improved methods that can be used to generate iPS cells, and also refer to the future challenges.
authors.affiliation: Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Mochiduki
authors.forename: Keisuke
authors.initials: K
authors.lastname: Okita
mesh_terms: D018929:Cell Culture Techniques; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D055808:Drug Discovery; D016259:Genes, myc; D016158:Genes, p53; D005822:Genetic Vectors; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000502:physiology*; D016086:Lentivirus / Q000235:genetics; D035683:MicroRNAs; D034741:RNA, Small Interfering; D012190:Retroviridae / Q000235:genetics; D029082:Sendai virus / Q000235:genetics; D057905:Stem Cell Research; D020825:T-Box Domain Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; C508055:GLIS1 protein, human; D035683:MicroRNAs; D034741:RNA, Small Interfering; D020825:T-Box Domain Proteins; C106884:TBX3 protein, human; D014157:Transcription Factors