title: Influenza virus inactivated by artificial ribonucleases as a prospective killed virus vaccine.
abstract: The inactivation of viral particles with agents causing minimal damage to the structure of surface epitopes is a well-established approach for the production of killed virus vaccines. Here, we describe new agents for the inactivation of influenza virus, artificial ribonucleases (aRNases), which are chemical compounds capable of cleaving RNA molecules. Several aRNases were identified, exhibiting significant virucidal activity against the influenza A virus and causing a minimal effect on the affinity of monoclonal antibodies for the inactivated virus. Using a murine model of the influenza virus infection, a high protective activity of the aRNase-inactivated virus as a vaccine was demonstrated. The results of the experiments demonstrate the efficacy of novel chemical agents in the preparation of vaccines against influenza and, perhaps, against other infections caused by RNA viruses.
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, SB RAS, 630090 Novosibirsk, Russian Federation.
authors.forename: Antonina A
authors.initials: AA
authors.lastname: Fedorova
authors.forename: Elena P
authors.initials: EP
authors.lastname: Goncharova
authors.forename: Mikhail P
authors.initials: MP
authors.lastname: Kovpak
authors.forename: Valentin V
authors.initials: VV
authors.lastname: Vlassov
authors.forename: Marina A
authors.initials: MA
authors.lastname: Zenkova
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D050296:Microbial Viability / Q000187:drug effects; D009976:Orthomyxoviridae Infections / Q000517:prevention & control; D020871:RNA Stability; D012367:RNA, Viral / Q000378:metabolism*; D012260:Ribonucleases / Q000378:metabolism*; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000276:immunology; D038301:Virus Inactivation
chemical_list: D007252:Influenza Vaccines; D012367:RNA, Viral; D015164:Vaccines, Inactivated; D012260:Ribonucleases