title: [Separation and purification process of total flavones from four-leaf diet decoction].
abstract: OBJECTIVE
  To investigate purification process of total flavonoids from four-leaf diet decoction with macroreticular resin.
  
  
  METHODS
  By using the static absorption tests and dynamic absorption tests with different types of macroreticular resins, the better parameters were found and the orthogonal design were used in purified craft.
  
  
  RESULTS
  AB-8 resin showed the best absorption property with the following technological conditions: the ratio of resin (g) - extract (mL) is 1.2: 1, the ratio of column diameter to height was 1: 12, then washed with water to colorless, and the volume of 80% ethanol was 7 BV.
  
  
  CONCLUSIONS
  The process is stable and the purity of total flavones is up to 85%.
authors.affiliation: Pharmacology College, Anhui Xinhua University, Hefei 230088, China. weiqiang509@sina.com
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wei
authors.forename: Wei
authors.initials: W
authors.lastname: Yin
authors.forename: Wen-Jing
authors.initials: WJ
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Wu
mesh_terms: D000327:Adsorption; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D000431:Ethanol / Q000737:chemistry; D047309:Flavones / Q000302:isolation & purification*; D000960:Hypolipidemic Agents / Q000737:chemistry / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry; D010945:Plants, Edible / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D012117:Resins, Synthetic / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013678:Technology, Pharmaceutical / Q000379:methods
chemical_list: D004365:Drugs, Chinese Herbal; D047309:Flavones; D000960:Hypolipidemic Agents; D012117:Resins, Synthetic; D000431:Ethanol