title: [Effect of 25-hydroxyvitamin D(3) on vitamin D receptor expression and distribution in human bronchial epithelial cells in vitro].
abstract: OBJECTIVE
  To evaluate the effect of 25-hydroxyvitamin D(3) on the expression and distribution of vitamin D receptor in normal human bronchial epithelial cells.
  
  
  METHODS
  MTT assay was used to assess the viability of human airway epithelial cell line 16HBE following a 24-h exposure to different concentrations of 25-hydroxyvitamin D(3). Real-time quantitative PCR, Western blotting, and immunofluorescence assay were used to observe the expression and distribution of vitamin D receptor in the cells following the exposure.
  
  
  RESULTS
  Compared with the control cells, 16HBE cells exposed to different concentrations of 25-hydroxyvitamin D(3) exhibited no significantly increase in the expression or distribution of vitamin D receptor.
  
  
  CONCLUSIONS
  The influence of 25-hydroxyvitamin D(3) on bronchial epithelial cells might be independent of the expression and translocation of vitamin D receptor.
authors.affiliation: Department of Respiratory Diseases, Southern Medical University, Guangzhou, China. huxidhm@126.com
authors.forename: Hangming
authors.initials: H
authors.lastname: Dong
authors.forename: Haijin
authors.initials: H
authors.lastname: Zhao
authors.forename: Laiyu
authors.initials: L
authors.lastname: Liu
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Liang
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Lv
authors.forename: Shaoxi
authors.initials: S
authors.lastname: Cai
mesh_terms: D001980:Bronchi / Q000166:cytology*; D002112:Calcifediol / Q000494:pharmacology*; D002460:Cell Line; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018167:Receptors, Calcitriol / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D018167:Receptors, Calcitriol; D002112:Calcifediol