title: Mono-2-ethylhexyl phthalate induced loss of mitochondrial membrane potential and activation of Caspase3 in HepG2 cells.
abstract: L02 and HepG2 cells were exposed to mono-(2-ethylhexyl) phthalate (MEHP) at concentrations of 6.25-100μM. After 48h treatment, MEHP decreased HepG2 cell viability in a concentration-dependent manner and L02 cell viability in the 50 and 100μM groups (p<0.01). Furthermore, at 24 and 48h after treatment, MEHP decreased the glutathione levels of HepG2 cells in all treatment groups and in the ΔΨ(m) in L02 and HepG2 cells with MEHP≥25μM (p<0.05 or p<0.01). At 24h after treatment, MEHP induced activation of caspase3 in all treated HepG2 and L02 cells (p<0.05 or p<0.01) except the 100μM MEHP treatment group. The increase in the Bax to Bcl-2 ratio suggests that Bcl-2 family involved in the control of MEHP-induced apoptosis in these two cell types. The data suggest that MEHP could induce apoptosis of HepG2 cells through mitochondria- and caspase3-dependent pathways.
authors.affiliation: Department of Occupational and Environmental Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, PR China.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.forename: Jianshu
authors.initials: J
authors.lastname: Wang
authors.forename: Qizhi
authors.initials: Q
authors.lastname: Qin
authors.forename: Ying
authors.initials: Y
authors.lastname: Jiang
authors.forename: Guangtao
authors.initials: G
authors.lastname: Yang
authors.forename: Kaimin
authors.initials: K
authors.lastname: Rao
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Xiong
authors.forename: Jing
authors.initials: J
authors.lastname: Yuan
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism*; D053453:Caspase 9 / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004051:Diethylhexyl Phthalate / Q000031:analogs & derivatives* / Q000633:toxicity; D005978:Glutathione / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: C494826:BAX protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D004051:Diethylhexyl Phthalate; D053148:Caspase 3; D053453:Caspase 9; C016599:mono-(2-ethylhexyl)phthalate; D005978:Glutathione