title: Multisite performance evaluation of an enzyme-linked immunosorbent assay for detection of Giardia, Cryptosporidium, and Entamoeba histolytica antigens in human stool.
abstract: A novel fecal antigen detection assay for fresh and frozen human samples that detects but does not differentiate Giardia spp, Cryptosporidium spp, and Entamoeba histolytica, the Tri-Combo parasite screen, was compared to three established enzyme-linked immunosorbent assays (ELISAs) at three international sites. It exhibited 97.9% sensitivity and 97.0% specificity, with positive and negative predictive values of 93.4% and 99.1%, respectively. The Tri-Combo test proved a reliable means to limit the use of individual parasite ELISAs to positive samples.
authors.affiliation: Division of Infectious Diseases and International Health, University of Virginia School of Medicine, Charlottesville, Virginia, USA.
authors.forename: Nathaniel C V
authors.initials: NC
authors.lastname: Christy
authors.forename: Janice D
authors.initials: JD
authors.lastname: Hencke
authors.forename: Aleyla
authors.initials: A
authors.lastname: Escueta-De Cadiz
authors.forename: Forida
authors.initials: F
authors.lastname: Nazib
authors.forename: Heidrun
authors.initials: H
authors.lastname: von Thien
authors.forename: Kenji
authors.initials: K
authors.lastname: Yagita
authors.forename: Segni
authors.initials: S
authors.lastname: Ligaba
authors.forename: Rashidul
authors.initials: R
authors.lastname: Haque
authors.forename: Tomoyoshi
authors.initials: T
authors.lastname: Nozaki
authors.forename: Egbert
authors.initials: E
authors.lastname: Tannich
authors.forename: Joel F
authors.initials: JF
authors.lastname: Herbein
authors.forename: William A
authors.initials: WA
authors.lastname: Petri
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000953:Antigens, Protozoan / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D019411:Clinical Laboratory Techniques / Q000379:methods*; D003458:Cryptosporidium / Q000302:isolation & purification*; D004748:Entamoeba histolytica / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005243:Feces / Q000469:parasitology*; D005260:Female; D005872:Giardia / Q000302:isolation & purification*; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D010274:Parasitology / Q000379:methods*; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: D000953:Antigens, Protozoan