title: Effect of microwave exposure on the ovarian development of Drosophila melanogaster.
abstract: In the present experiments the effect of GSM radiation on ovarian development of virgin Drosophila melanogaster female insects was studied. Newly emerged adult female flies were collected and divided into separate identical groups. After the a lapse of certain number of hours-different for each group-the insects (exposed and sham-exposed) were dissected and their intact ovaries were collected and photographed under an optical microscope with the same magnification. The size of the ovaries was compared between exposed and sham-exposed virgin female insects, during the time needed for the completion of oogenesis and maturation of the first eggs in the ovarioles. Immediately after the intact ovaries were photographed, they were further dissected into individual ovarioles and treated for TUNEL and acridine-orange assays to determine the degree of DNA damage in the egg chamber cells. The study showed that the ovarian size of the exposed insects is significantly smaller than that of the corresponding sham-exposed insects, due to destruction of egg chambers by the GSM radiation, after DNA damage and consequent cell death induction in the egg chamber cells of the virgin females as shown in previous experiments on inseminated females. The difference in ovarian size between sham-exposed and exposed virgin female flies becomes most evident 39-45 h after eclosion when the first eggs within the ovaries are at the late vitellogenic and post-vitellogenic stages (mid-late oogenesis). More than 45 h after eclosion, the difference in ovarian size decreases, as the first mature eggs of the sham-exposed insects are leaving the ovaries and are laid.
authors.affiliation: Section of Cell Biology and Biophysics, Department of Biology, University of Athens, Panepistimiopolis, 15784 Athens, Greece. dpanagop@biol.uoa.gr
authors.forename: Dimitris J
authors.initials: DJ
authors.lastname: Panagopoulos
mesh_terms: D000818:Animals; D040421:Cell Phone; D053938:DNA Fragmentation; D004331:Drosophila melanogaster / Q000254:growth & development* / Q000528:radiation effects; D004781:Environmental Exposure; D005260:Female; D008872:Microwaves / Q000009:adverse effects*; D009866:Oogenesis / Q000528:radiation effects; D009929:Organ Size / Q000528:radiation effects; D010053:Ovary / Q000254:growth & development* / Q000528:radiation effects; D011679:Pupa / Q000528:radiation effects