title: [Study of additive properties of total quantum statistical moment of chromatographic fingerprint for Buyang Huanwu decoction].
abstract: OBJECTIVE
  To study the differences of HPLC fingerprints with the total quantum statistical moment for the Buyang Huanwu decoction (BYHWD) processed by various ways, and to verify the additive properties of total quantum statistical moment.
  
  
  METHODS
  The extracts of BYHWD were obtained by water extraction and alcohol precipitation firstly, and then were dissolved with five solvents of different solubility parameter from 11.4 Cal(1/2) x cm(-3/2) to 23.40 Cal(1/2) x cm(-3/2) respectively. Their chromatographic fingerprints were determined by HPLC, finally the total quantum statistical moment parameters and its superposition properties were manual calculated and analyzed by their expressions.
  
  
  RESULTS
  As a contrast as the whole prescription, the similarities of the various processed samples with butanol (11.4 Cal(1/2) x cm(-3/2)), methanol (13.5 Cal(1/2) x cm(-3/2)), 68% methanol (16. 67 Cal(1/2) x cm(-3/2)), 34% methanol (20.03 Cal(1/2) x cm(-3/2)) and distilled water (23.40 Cal(1/2) x cm(-3/2)) were 0. 074, 0. 973, 0. 934, 0. 991, 0. 993, respectively and while the RSD of these total quantum zero moment, center moment and variance of each chromatographic fingerprints for them were 63.04%, 16.22%, 69.38%, which showed significant difference in these chromatographic fingerprints. The total quantum statistical moment parameters of the superimposed chromatographic fingerprint with each sole samples were 3.203 x 10(5) mAu x s, 29.85 min, 389.97 min2, whereas the whole prescription's were 6.548 x 10(4) mAu x s, 29.44 min, 389.00 min2, that suggested that the absolute difference percentages between the superimposed and the whole were 2.209%, 1.389%, 0.2484%, respectively.
  
  
  CONCLUSIONS
  The total quantum statistical moment of the chromatographic fingerprints is of characteristics with additive properties, it can be used in static and dynamic quality controlled analyses in the Chinese medicine multiple component systems.
authors.affiliation: Department of Pharmaceutics, Hunan University of Traditional Chinese Medicine, Changsha 410208, China. xiaopeng-duan@163.com
authors.forename: Xiaopeng
authors.initials: X
authors.lastname: Duan
authors.forename: Fuyuan
authors.initials: F
authors.lastname: He
authors.forename: Jin
authors.initials: J
authors.lastname: Zhou
authors.forename: Jiaoli
authors.initials: J
authors.lastname: Zeng
authors.forename: Xianggui
authors.initials: X
authors.lastname: Xie
authors.forename: Haiqin
authors.initials: H
authors.lastname: Wang
mesh_terms: D001699:Biometry / Q000379:methods*; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D012995:Solubility
chemical_list: D004365:Drugs, Chinese Herbal; C460102:buyang huanwu