title: Two-dimensional proteome reference map of Vibrio tapetis, the aetiological agent of brown ring disease in clams.
abstract: OBJECTIVE
  Vibrio tapetis is the etiological agent of brown ring disease (BRD) in clams, one of the most threatening diseases affecting this commercially important bivalve. In this study we have constructed a proteome reference map of the V. tapetis type strain CECT 4600(T).
  
  
  RESULTS
  Eighty-two proteins, consistently present in all 2D-gels, were identified by mass spectrometry or by de novo sequencing. The majority of the proteins identified (66%) belonged to four COG categories: 'Carbohydrate transport and metabolism', 'Post-translational modification, protein turnover and chaperones', 'Energy production', and 'Amino acid transport and metabolism'. Glyceraldehyde-3-phosphate dehydrogenase, enolase, fructose-bisphosphate aldolase, phosphoglycerate kinase. molecular chaperones Dnak and GroEL, alkyl hydroperoxide reductase, peptidyl-prolyl cis-trans isomerase B and factor Tu, were identified among the 20 most abundant proteins. A comparison of this reference map with that obtained for the V. tapetis strain GR0202RD, with different origin and pathophysiological characteristics, was performed.
  
  
  CONCLUSIONS
  Under the culture conditions employed in this study, glucose degradation is one of the major pathways for energy production in Vibrio tapetis. In addition, the two strains studied, although with remarkable differences at genetic and pathophysiological levels, showed a high similarity under laboratory conditions.
  
  
  CONCLUSIONS
  The results obtained here can be considered as a first step to gather valuable information on protein expression, related not only to diverse cellular functions and regulation but also to pathogenesis and bacterium-host interactions in the disease process.
authors.affiliation: Departamento de Genética, Universidad de Santiago de Compostela, Santiago de Compostela, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Bermúdez-Crespo
authors.forename: S
authors.initials: S
authors.lastname: Balboa
authors.forename: J
authors.initials: J
authors.lastname: Alonso
authors.forename: J L
authors.initials: JL
authors.lastname: Romalde
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis*; D049872:Bivalvia / Q000382:microbiology*; D015180:Electrophoresis, Gel, Two-Dimensional; D004734:Energy Metabolism; D013058:Mass Spectrometry; D020543:Proteome / Q000032:analysis*; D020539:Sequence Analysis, Protein; D014733:Vibrio / Q000737:chemistry* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D020543:Proteome