title: Identification of a novel mutation in FAD2B from a peanut EMS mutant with elevated oleate content.
abstract: A Virginia type peanut mutant with more than 60% oleate content (E2-4-83-12) was selected from an EMS mutagenized population of LF2 (an export type peanut cultivar with 44.2% oleate) by near infrared reflectance spectroscopy. Cloning and sequencing of FAD2B from LF2 and E2-4-83-12 identified a novel mutation (C313T in the coding region) causing an H105Y substitution in the first histidine box of the FAD2B protein. GC-MS analysis of fatty acids in yeast cells harboring pYES2 with the mutated FAD2B detected no linoleate, confirming that FAD2B from E2-4-83-12 was dysfunctional. Loss-of-function FAD2A and FAD2B together contributed to elevated oleate phenotype of the peanut EMS mutant.
authors.affiliation: Shandong Peanut Research Institute, Qingdao, China.
authors.forename: Chao Qi
authors.initials: CQ
authors.lastname: Fang
authors.forename: Chuan Tang
authors.initials: CT
authors.lastname: Wang
authors.forename: Pi Wu
authors.initials: PW
authors.lastname: Wang
authors.forename: Yue Yi
authors.initials: YY
authors.lastname: Tang
authors.forename: Xiu Zhen
authors.initials: XZ
authors.lastname: Wang
authors.forename: Feng Gao
authors.initials: FG
authors.lastname: Cui
authors.forename: Shan Lin
authors.initials: SL
authors.lastname: Yu
mesh_terms: D000383:Agriculture; D000595:Amino Acid Sequence; D010367:Arachis / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D005020:Ethyl Methanesulfonate / Q000633:toxicity*; D044943:Fatty Acid Desaturases / Q000737:chemistry / Q000235:genetics*; D008401:Gas Chromatography-Mass Spectrometry; D017343:Genes, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D016296:Mutagenesis / Q000187:drug effects / Q000235:genetics; D009154:Mutation / Q000235:genetics*; D019301:Oleic Acid / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D019265:Spectroscopy, Near-Infrared
chemical_list: D019301:Oleic Acid; D005020:Ethyl Methanesulfonate; D044943:Fatty Acid Desaturases