title: [Pancreatic secretory trypsin inhibitor gene N34S mutation in patients with idiopathic chronic pancreatitis].
abstract: OBJECTIVE
  To determine the frequency and prognostic significance of mutation N34S in the development of CP in a mixed Russian population.
  
  
  METHODS
  The study included 83 patients with idiopathic CP and 103 healthy individuals. The diagnosis of CP was based on clinical and instrumental data in accordance with the criteria of M-ANNHEIM. Everyone involved in this study using PCR followed by restriction analysis performed the N34S mutation analysis in the gene for the ICU.
  
  
  RESULTS
  N34S mutation was detected in 12 patients with CP and in 3 of the control group, which accounted for 14.6 and 2.9% respectively; p<0,05. The odds ratio (OR) of idiopathic CP in the presence of mutation N34S was 4.62 (95% confidence interval (CI): 1,21-18,37). The difference in frequency of homozygotes the mutant allele was statistically significant: 10.97 and 0.97% in the main and control groups, respectively; p<0,05. The presence of mutations did not affect the frequency and severity of functional disorders of the pancreas, but the mutation N34S was statistically significantly more common in patients with calcifying pancreatitis (p<0,01) and the presence of pseudocysts (p<0,05). OR the development of calcification in patients with the presence of N345 was 13.44 (95% CI: 6,29-23,78).
  
  
  CONCLUSIONS
  These data suggest that the mutation N34S plays a significant role in the development of CP in the Russian population and can detect more than 10% of cases of idiopathic CP.
authors.forename: Iu A
authors.initials: IuA
authors.lastname: Kucheriavyĭ
authors.forename: N V
authors.initials: NV
authors.lastname: Petrova
authors.forename: Z F
authors.initials: ZF
authors.lastname: Tibilova
authors.forename: A V
authors.initials: AV
authors.lastname: Smirnov
authors.forename: T S
authors.initials: TS
authors.lastname: Oganesian
authors.forename: A N
authors.initials: AN
authors.lastname: Kaziulin
authors.forename: I V
authors.initials: IV
authors.lastname: Maev
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002352:Carrier Proteins / Q000235:genetics*; D016022:Case-Control Studies; D003627:Data Interpretation, Statistical; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D050500:Pancreatitis, Chronic / Q000175:diagnosis / Q000453:epidemiology / Q000235:genetics*; D012426:Russia; D014359:Trypsin Inhibitor, Kazal Pancreatic
chemical_list: D002352:Carrier Proteins; C486642:SPINK1 protein, human; D014359:Trypsin Inhibitor, Kazal Pancreatic