title: Longitudinal tracking of human fetal cells labeled with super paramagnetic iron oxide nanoparticles in the brain of mice with motor neuron disease.
abstract: Stem Cell (SC) therapy is one of the most promising approaches for the treatment of Amyotrophic Lateral Sclerosis (ALS). Here we employed Super Paramagnetic Iron Oxide nanoparticles (SPIOn) and Hoechst 33258 to track human Amniotic Fluid Cells (hAFCs) after transplantation in the lateral ventricles of wobbler (a murine model of ALS) and healthy mice. By in vitro, in vivo and ex vivo approaches we found that: 1) the main physical parameters of SPIOn were maintained over time; 2) hAFCs efficiently internalized SPIOn into the cytoplasm while Hoechst 33258 labeled nuclei; 3) SPIOn internalization did not alter survival, cell cycle, proliferation, metabolism and phenotype of hAFCs; 4) after transplantation hAFCs rapidly spread to the whole ventricular system, but did not migrate into the brain parenchyma; 5) hAFCs survived for a long time in the ventricles of both wobbler and healthy mice; 6) the transplantation of double-labeled hAFCs did not influence mice survival.
authors.affiliation: Mario Negri Institute for Pharmacological Research, Milan, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Bigini
authors.forename: Valentina
authors.initials: V
authors.lastname: Diana
authors.forename: Sara
authors.initials: S
authors.lastname: Barbera
authors.forename: Elena
authors.initials: E
authors.lastname: Fumagalli
authors.forename: Edoardo
authors.initials: E
authors.lastname: Micotti
authors.forename: Leopoldo
authors.initials: L
authors.lastname: Sitia
authors.forename: Alessandra
authors.initials: A
authors.lastname: Paladini
authors.forename: Cinzia
authors.initials: C
authors.lastname: Bisighini
authors.forename: Laura
authors.initials: L
authors.lastname: De Grada
authors.forename: Laura
authors.initials: L
authors.lastname: Coloca
authors.forename: Laura
authors.initials: L
authors.lastname: Colombo
authors.forename: Pina
authors.initials: P
authors.lastname: Manca
authors.forename: Patrizia
authors.initials: P
authors.lastname: Bossolasco
authors.forename: Francesca
authors.initials: F
authors.lastname: Malvestiti
authors.forename: Fabio
authors.initials: F
authors.lastname: Fiordaliso
authors.forename: Gianluigi
authors.initials: G
authors.lastname: Forloni
authors.forename: Massimo
authors.initials: M
authors.lastname: Morbidelli
authors.forename: Mario
authors.initials: M
authors.lastname: Salmona
authors.forename: Daniela
authors.initials: D
authors.lastname: Giardino
authors.forename: Tiziana
authors.initials: T
authors.lastname: Mennini
authors.forename: Davide
authors.initials: D
authors.lastname: Moscatelli
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Silani
authors.forename: Lidia
authors.initials: L
authors.lastname: Cova
mesh_terms: D000653:Amniotic Fluid / Q000166:cytology*; D000690:Amyotrophic Lateral Sclerosis / Q000235:genetics*; D000818:Animals; D006690:Bisbenzimidazole / Q000494:pharmacology; D001921:Brain / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D049109:Cell Proliferation; D002469:Cell Separation; D002470:Cell Survival; D004195:Disease Models, Animal; D005290:Ferric Compounds / Q000494:pharmacology*; D053686:Fetal Stem Cells / Q000166:cytology*; D005434:Flow Cytometry; D006579:Heterozygote; D006801:Humans; D008027:Light; D008279:Magnetic Resonance Imaging / Q000379:methods; D008280:Magnetics; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D051379:Mice; D046529:Microscopy, Electron, Transmission / Q000379:methods; D010641:Phenotype; D012542:Scattering, Radiation; D013997:Time Factors
chemical_list: D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; C000499:ferric oxide; D006690:Bisbenzimidazole