title: Purification and characterization of a nitric oxide inhibitory peptide from Ruditapes philippinarum.
abstract: Ruditapes philippinarum (R. philippinarum) were hydrolyzed using 8 proteases to produce an anti-inflammatory peptide of the various hydrolysates produced, the Alcalase hydrolysate exhibited the highest nitric oxide (NO) inhibitory activity. The derived peptide was purified using high performance liquid chromatography (HPLC) and NO-inhibitory activity of the purified compound was evaluated. The sequence of the NO-inhibitory peptide obtained was composed of 10 amino acid residues, Gln-Cys-Gln-Gln-Ala-Val-Gln-Ser-Ala-Val at N-terminal position. In addition, we investigated the inhibitory effect of the purified peptide from R. philippinarum on NO production in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. In this analysis the purified peptide from R. philippinarum was shown to inhibit LPS-induced NO production in RAW264.7 cells. The present results indicate that the purified peptide displayed potent anti-inflammation activity in RAW264.7 cells.
authors.affiliation: Department of Biotechnology, Konkuk University, Chungju 380-701, Republic of Korea.
authors.forename: Seung-Jae
authors.initials: SJ
authors.lastname: Lee
authors.forename: Eun-Kyung
authors.initials: EK
authors.lastname: Kim
authors.forename: Yon-Suk
authors.initials: YS
authors.lastname: Kim
authors.forename: Jin-Woo
authors.initials: JW
authors.lastname: Hwang
authors.forename: Kwang Ho
authors.initials: KH
authors.lastname: Lee
authors.forename: Dong-Kug
authors.initials: DK
authors.lastname: Choi
authors.forename: Hyun
authors.initials: H
authors.lastname: Kang
authors.forename: Sang-Ho
authors.initials: SH
authors.lastname: Moon
authors.forename: Byong-Tae
authors.initials: BT
authors.lastname: Jeon
authors.forename: Pyo-Jam
authors.initials: PJ
authors.lastname: Park
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D049872:Bivalvia / Q000737:chemistry*; D015153:Blotting, Western; D002460:Cell Line; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D051546:Cyclooxygenase 2 / Q000378:metabolism; D006868:Hydrolysis; D051379:Mice; D009569:Nitric Oxide / Q000037:antagonists & inhibitors*; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D000893:Anti-Inflammatory Agents; D010455:Peptides; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2