title: [Determination of propofol in human blood by GC-MS].
abstract: OBJECTIVE
  To establish a gas chromatography-mass spectrometry (GC-MS) method for determination of propofol in human blood.
  
  
  METHODS
  Propofol and 2-(tert-Butyl)-4,6-dimethylphenol (internal standard) were isolated from human blood samples with liquid-liquid ether extraction. The organic layer was collected after centrifugation and dried using the water bath. The extracted residue was analyzed by GC-MS.
  
  
  RESULTS
  Propofol and the internal standard showed a good separation with a good linear concentration ranged from 0.02 to 10 microg/mL in blood. The linear function was y = 0.313 6 x-0.006 8 with the correlation coefficient of 0.9997. The precision of intra-day and inter-day were less than 4.8% and the lower limit of detection of propofol was 0.005 microg/mL. Propofol concentration of blood was 0.14 microg/mL using this method in the practice work.
  
  
  CONCLUSIONS
  The GC-MS method is rapid, sensitive, reliable and suitable for qualitative and quantitative analysis propofol of blood in forensic toxicological analysis and clinical drug monitoring.
authors.affiliation: Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, PR China, Shanghai 200063, China. sunyingying1986@sina.com
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Sun
authors.forename: Ping
authors.initials: P
authors.lastname: Xiang
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Bu
authors.forename: Min
authors.initials: M
authors.lastname: Shen
mesh_terms: D018686:Anesthetics, Intravenous / Q000097:blood* / Q000737:chemistry / Q000506:poisoning; D016903:Drug Monitoring / Q000379:methods; D053593:Forensic Toxicology / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D008297:Male; D015394:Molecular Structure; D015742:Propofol / Q000097:blood* / Q000737:chemistry / Q000506:poisoning; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D018686:Anesthetics, Intravenous; D015742:Propofol