title: Solution-phase synthesis of branched DNA hybrids via H-phosphonate dimers.
abstract: A method for the solution-phase synthesis of branched oligonucleotides with tetrahedral or pseudo-octahedral geometry is described that involves the coupling of 3'-H-phosphonates of protected dinucleoside phosphates and organic core molecules. The dimer building blocks are produced by a synthesis that requires no chromatographic purification and that produces the dimer H-phosphonates in up to 44% yield in less than three days of laboratory work. A total of seven different branched hybrids were prepared, including a new hybrid of the sequence (CG)(4)TBA, where TBA stands for tetrakis(p-hydroxybiphenyl)adamantane that assembles into a material from micromolar aqueous solution upon addition of MgCl(2).
authors.affiliation: Institute for Organic Chemistry, University of Stuttgart, 70569 Stuttgart, Germany.
authors.forename: Arunoday
authors.initials: A
authors.lastname: Singh
authors.forename: Mariyan
authors.initials: M
authors.lastname: Tolev
authors.forename: Christine I
authors.initials: CI
authors.lastname: Schilling
authors.forename: Stefan
authors.initials: S
authors.lastname: Bräse
authors.forename: Helmut
authors.initials: H
authors.lastname: Griesser
authors.forename: Clemens
authors.initials: C
authors.lastname: Richert
mesh_terms: D000218:Adamantane / Q000031:analogs & derivatives* / Q000737:chemistry*; D004247:DNA / Q000138:chemical synthesis* / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D063065:Organophosphonates / Q000737:chemistry*; D012996:Solutions / Q000737:chemistry*
chemical_list: D009841:Oligonucleotides; D063065:Organophosphonates; D012996:Solutions; D004247:DNA; D000218:Adamantane