title: Detection and characterization of VIM-31, a new variant of VIM-2 with Tyr224His and His252Arg mutations, in a clinical isolate of Enterobacter cloacae.
abstract: We report the first description of the metallo-β-lactamase VIM-31, a new variant of VIM-2 with Tyr224His and His252Arg mutations, in Enterobacter cloacae 11236, which was isolated from blood specimens of a patient with colonic adenocarcinoma in Belgium. bla(VIM-31) was found on a class 1 integron located on a self-transferable but not typeable 42-kb plasmid. Compared to values published elsewhere for VIM-2, the purified VIM-31 enzyme showed weaker catalytic efficiency against all the tested beta-lactam agents (except for ertapenem), resulting from lower k(cat) (except for ertapenem) and higher K(m) values for VIM-31.
authors.affiliation: Laboratory of Bacteriology, CHU Mont-Godinne, Université Catholique de Louvain, Yvoir, Belgium.
authors.forename: Pierre
authors.initials: P
authors.lastname: Bogaerts
authors.forename: Carine
authors.initials: C
authors.lastname: Bebrone
authors.forename: Te-Din
authors.initials: TD
authors.lastname: Huang
authors.forename: Warda
authors.initials: W
authors.lastname: Bouchahrouf
authors.forename: Yves
authors.initials: Y
authors.lastname: Degheldre
authors.forename: Ariane
authors.initials: A
authors.lastname: Deplano
authors.forename: Kurt
authors.initials: K
authors.lastname: Hoffmann
authors.forename: Youri
authors.initials: Y
authors.lastname: Glupczynski
mesh_terms: D016521:Electrophoresis, Gel, Pulsed-Field; D018274:Electroporation; D016972:Enterobacter cloacae / Q000201:enzymology*; D007700:Kinetics; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001618:beta-Lactamases