title: Remarkably high rate of DNA amplification promoted by the mating-type switching mechanism in Schizosaccharomyces pombe.
abstract: A novel mating-type switching-defective mutant showed a highly unstable rearrangement at the mating-type locus (mat1) in fission yeast. The mutation resulted from local amplification of a 134-bp DNA fragment by the mat1-switching phenomenon. We speculate that the rolling-circle-like replication and homologous recombination might be the general mechanisms for local genome region expansion.
authors.affiliation: Developmental Genetics Section, Gene Regulation and Chromosome Biology Laboratory, National Institutes of Health, National Cancer Institute, Frederick, Maryland 21702-1201, USA.
authors.forename: Chuanhe
authors.initials: C
authors.lastname: Yu
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Bonaduce
authors.forename: Amar J S
authors.initials: AJ
authors.lastname: Klar
mesh_terms: D000483:Alleles; D001483:Base Sequence; D004271:DNA, Fungal / Q000235:genetics*; D005784:Gene Amplification; D049770:Genes, Mating Type, Fungal / Q000235:genetics*; D008969:Molecular Sequence Data; D059645:Mutation Rate; D010641:Phenotype; D012568:Schizosaccharomyces / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics
chemical_list: D004271:DNA, Fungal; D029702:Schizosaccharomyces pombe Proteins