title: Electrosprayed hydroxyapatite on polymer nanofibers to differentiate mesenchymal stem cells to osteogenesis.
abstract: Electrospraying of hydroxyapatite (HA) nanoparticles onto the surface of polymer nanofibers provides a potentially novel substrate for the adhesion, proliferation and differentiation of mesenchymal stem cells (MSCs) into bone tissue regeneration. HA nanoparticles (4%) were electrosprayed on the surface of electrospun polycaprolactone (PCL) nanofibers (420 ± 15 nm) for bone tissue engineering. PCL/HA nanofibers were comparatively characterized with PCL/Collagen (275 ± 56 nm) nanofibers by FT-IR analysis to confirm the presence of HA. Fabricated PCL/HA and PCL/Collagen nanofibers and TCP (control) were used for the differentiation of equine MSC into osteogenic lineages in the presence of DMEM/F12 medium supplemented with β-glycerophosphate, ascorbic acid and dexamethasone. Cell proliferation and differentiation into an osteogenic lineage was evaluated by MTS assay, SEM observation, ALP activity, ARS staining, quantification of mineral deposition and expression of osteocalcin. Proliferation of MSCs increased significantly (P ⩽ 0.05) up to 12% in PCL/Collagen (day 15) compared to PCL/HA nanofibrous substrate. ALP activity was increased 20% in PCL/HA by day 10 confirming the direction of osteogenic lineage from MSCs differentiation. PCL/HA stimulated an increased mineral secretion up to 26% by day 15 on ARS staining compared to PCL/Collagen nanofibers and showing cuboidal morphology by expressing osteocalcin. These results confirmed that the specifically fabricated PCL/HA composite nanofibrous substrate enhanced the differentiation of MSCs into osteogenesis.
authors.affiliation: Healthcare and Energy Materials Laboratory, Faculty of Engineering, National University of Singapore, Singapore. nnijrv@nus.edu.sg
authors.forename: J
authors.initials: J
authors.lastname: Venugopal
authors.forename: R
authors.initials: R
authors.lastname: Rajeswari
authors.forename: M
authors.initials: M
authors.lastname: Shayanti
authors.forename: Sharon
authors.initials: S
authors.lastname: Low
authors.forename: Ariff
authors.initials: A
authors.lastname: Bongso
authors.forename: V R Giri
authors.initials: VR
authors.lastname: Dev
authors.forename: G
authors.initials: G
authors.lastname: Deepika
authors.forename: Aw Tar
authors.initials: AT
authors.lastname: Choon
authors.forename: S
authors.initials: S
authors.lastname: Ramakrishna
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002454:Cell Differentiation; D002478:Cells, Cultured; D017886:Durapatite / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000295:instrumentation; D004867:Equipment Design; D006736:Horses; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D010012:Osteogenesis; D011091:Polyesters / Q000737:chemistry*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; C016240:polycaprolactone; D017886:Durapatite; D000469:Alkaline Phosphatase