title: Plasma clusterin increased prior to small for gestational age (SGA) associated with preeclampsia and decreased prior to SGA in normotensive pregnancies.
abstract: In our search for early biomarkers for the pregnancy complicationssmall for gestational age (SGA) and preeclampsia (PE) we analysed plasma from 19-21 weeks gestation in women recruited into the SCOPE study, a prospective cohort of nulliparous women, by differential in gel electrophoresis (DIGE). DIGE revealed the differential expression of clusterin levels and its isoforms in top6-depleted plasma of women who delivered an SGA infant but remained normotensive (SGA-NT; N = 8) compared to healthy women with an uncomplicated pregnancy outcome (Controls, N = 8). Immunosorbent enzyme-linked assay (ELISA) showed that compared to plasma clusterin levels from healthy controls [71.1 (SD 12.4) µg/mL, n = 39], clusterin was decreased in SGA-NT [58.3 (SD 11.7), N = 20, P < 0.0001], increased in women with SGA and PE [81.5 (SD 14.8), N = 20, P < 0.01], but similar in PE alone [71.2 (SD 9.4)g/ml, P = 1.0]. Screening for clusterin levels and/or its different isoformsmay be useful in mid-pregnancy to identify women who subsequently develop SGA but remain normotensive or who develop preeclampsia with SGA.
authors.affiliation: School of Biological Sciences, Faculty of Science, University of Auckland, Auckland, New Zealand. m.blumenstein@auckland.ac.nz
authors.forename: Marion
authors.initials: M
authors.lastname: Blumenstein
authors.forename: Lesley M E
authors.initials: LM
authors.lastname: McCowan
authors.forename: Steven
authors.initials: S
authors.lastname: Wu
authors.forename: Garth J S
authors.initials: GJ
authors.lastname: Cooper
authors.forename: Robyn A
authors.initials: RA
authors.lastname: North
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood*; D051152:Clusterin / Q000097:blood*; D005260:Female; D005865:Gestational Age; D006801:Humans; D007231:Infant, Newborn; D007236:Infant, Small for Gestational Age / Q000097:blood*; D011225:Pre-Eclampsia / Q000097:blood*; D011247:Pregnancy; D011446:Prospective Studies
chemical_list: D015415:Biomarkers; D051152:Clusterin