title: Interactions of thyroid hormone receptor with Ku proteins and interleukin enhancer binding factor 3 modulate the promoter activity of thyroid-stimulating hormone alpha.
abstract: We previously identified Ku proteins and interleukin enhancer binding factor 3 (ILF3) as cofactors for the nuclear receptor farnesoid X receptor and liver receptor homolog-1, respectively. Here we provide further evidence that these cofactors modulate the promoter activity of the nuclear receptor thyroid hormone receptor (TR) target gene, thyroid-stimulating hormone alpha (TSHα), which is negatively regulated by the TR ligand triiodothyronine (T(3)). Ku proteins suppressed TSHα promoter activity independent of T(3), whereas ILF3 enhanced TSHα activity, especially in the presence of T(3). Taken together, our results suggest that Ku proteins and ILF3 function as co-regulators for TR-mediated TSHα expression.
authors.affiliation: Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Nagoya City University, Mizuho-ku, Nagoya, Aichi, Japan.
authors.forename: Masae
authors.initials: M
authors.lastname: Ohno
authors.forename: Mayu
authors.initials: M
authors.lastname: Fujita
authors.forename: Makoto
authors.initials: M
authors.lastname: Nishizuka
authors.forename: Shigehiro
authors.initials: S
authors.lastname: Osada
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Imagawa
mesh_terms: D004265:DNA Helicases / Q000378:metabolism*; D051747:DNA-Activated Protein Kinase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D015292:Glycoprotein Hormones, alpha Subunit / Q000235:genetics / Q000378:metabolism*; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D000072200:Ku Autoantigen; D050779:Nuclear Factor 90 Proteins / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011988:Receptors, Thyroid Hormone / Q000378:metabolism*; D014284:Triiodothyronine / Q000378:metabolism
chemical_list: D015292:Glycoprotein Hormones, alpha Subunit; C429643:ILF3 protein, human; D050779:Nuclear Factor 90 Proteins; D009687:Nuclear Proteins; D011988:Receptors, Thyroid Hormone; D014284:Triiodothyronine; D005982:Glutathione Transferase; D051747:DNA-Activated Protein Kinase; C497263:PRKDC protein, human; D004265:DNA Helicases; C106893:XRCC5 protein, human; D000072200:Ku Autoantigen