title: The expression of sex steroid synthesis and inactivation enzymes in subcutaneous adipose tissue of PCOS patients.
abstract: Modulation of sex steroid pre-receptor in adipose tissue is important for the development of metabolic diseases, but its roles in the pathogenesis of polycystic ovary syndrome (PCOS) has not been fully characterized. Herein we compared the expression of key sex steroid converting enzymes in the subcutaneous adipose tissue (SAT) between patients with PCOS and the matched controls. Most of the sex steroid converting enzymes were highly expressed in the SAT, except 17α-hydroxylase (CYP17A1). Compared with the controls, PCOS patients showed significantly higher levels of 3β-hydroxysteroid dehydrogenase1-2 (3β-HSD1-2), aldo-keto reductase 1C 1-3 (AKR1C1-3) and leptin, but lower level of P450 aromatase and 5α-reductase 1. Interestingly, leptin was positively correlated to AKR1C2 expression and negatively to 5α-reductase1 as well as peroxisome proliferator-activated receptor γ (PPARγ). In summary, the expression of enzymes synthesizing testosterone and enzymes inactivating DHT and progesterone was higher in SAT of PCOS patients compared to controls. Correlation analysis indicated that increased leptin expression may be negatively related to local DHT level. These data suggested that sex steroid converting enzymes expression was different in SAT of PCOS patients that might contribute to abnormal testosterone and leptin level of PCOS patients.
authors.affiliation: Department of Endocrinology, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200127, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Wang
authors.forename: Shengxian
authors.initials: S
authors.lastname: Li
authors.forename: Aimin
authors.initials: A
authors.lastname: Zhao
authors.forename: Tao
authors.initials: T
authors.lastname: Tao
authors.forename: Xiuying
authors.initials: X
authors.lastname: Mao
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
mesh_terms: D052242:Adiponectin / Q000235:genetics; D000273:Adipose Tissue / Q000378:metabolism*; D000328:Adult; D013196:Dihydrotestosterone / Q000378:metabolism; D005260:Female; D006801:Humans; D020738:Leptin / Q000235:genetics; D010088:Oxidoreductases / Q000235:genetics*; D047495:PPAR gamma / Q000235:genetics; D011085:Polycystic Ovary Syndrome / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D013739:Testosterone / Q000235:genetics; D055815:Young Adult
chemical_list: D052242:Adiponectin; D020738:Leptin; D047495:PPAR gamma; D012333:RNA, Messenger; D013196:Dihydrotestosterone; D013739:Testosterone; D010088:Oxidoreductases