title: Rapid detection of Betacoronavirus 1 from clinical fecal specimens by a novel reverse transcription loop-mediated isothermal amplification assay.
abstract: Betacoronavirus 1 (BCoV-1) is an important pathogen causing diarrhea in calves. In the current study, a novel reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid detection of BCoV-1 was successfully developed. The primers were designed to target the highly conserved fragment of BCoV-1 nucleocapsid gene. The assay displayed high specificity detecting only BCoV-1 with no cross reaction with other viruses. When 418 clinical samples from 6 different geographical areas of Xinjiang province were tested by the RT-LAMP method, the results indicated that this test is a simple, rapid, accurate, and sensitive method for the detection of BCoV-1.
authors.affiliation: College of Animal Science and Technology, Shihezi University, North Street No.4, Shihezi City, Xinjiang 832003, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Qiao
authors.forename: Qingling
authors.initials: Q
authors.lastname: Meng
authors.forename: Xuepeng
authors.initials: X
authors.lastname: Cai
authors.forename: Chuangfu
authors.initials: C
authors.lastname: Chen
authors.forename: Zaichao
authors.initials: Z
authors.lastname: Zhang
authors.forename: Zhenzhong
authors.initials: Z
authors.lastname: Tian
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000235:genetics / Q000821:virology*; D018352:Coronavirus Infections / Q000175:diagnosis / Q000235:genetics / Q000662:veterinary* / Q000821:virology; D017938:Coronavirus, Bovine / Q000235:genetics*; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D005243:Feces / Q000821:virology*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods / Q000662:veterinary*