title: Yeast mitochondrial leucyl-tRNA synthetase CP1 domain has functionally diverged to accommodate RNA splicing at expense of hydrolytic editing.
abstract: The yeast mitochondrial leucyl-tRNA synthetase (ymLeuRS) performs dual essential roles in group I intron splicing and protein synthesis. A specific LeuRS domain called CP1 is responsible for clearing noncognate amino acids that are misactivated during aminoacylation. The ymLeuRS CP1 domain also plays a critical role in splicing. Herein, the ymLeuRS CP1 domain was isolated from the full-length enzyme and was active in RNA splicing in vitro. Unlike its Escherichia coli LeuRS CP1 domain counterpart, it failed to significantly hydrolyze misaminoacylated tRNA(Leu). In addition and in stark contrast to the yeast domain, the editing-active E. coli LeuRS CP1 domain failed to recapitulate the splicing activity of the full-length E. coli enzyme. Although LeuRS-dependent splicing activity is rooted in an ancient adaptation for its aminoacylation activity, these results suggest that the ymLeuRS has functionally diverged to confer a robust splicing activity. This adaptation could have come at some expense to the protein's housekeeping role in aminoacylation and editing.
authors.affiliation: Department of Biochemistry, University of Illinois Urbana-Champaign, Urbana, Illinois 61801, USA.
authors.forename: Jaya
authors.initials: J
authors.lastname: Sarkar
authors.forename: Kiranmai
authors.initials: K
authors.lastname: Poruri
authors.forename: Michal T
authors.initials: MT
authors.lastname: Boniecki
authors.forename: Katherine K
authors.initials: KK
authors.lastname: McTavish
authors.forename: Susan A
authors.initials: SA
authors.lastname: Martinis
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000096:biosynthesis* / Q000235:genetics; D024101:Mitochondrial Proteins / Q000096:biosynthesis* / Q000235:genetics; D017434:Protein Structure, Tertiary; D012313:RNA / Q000235:genetics / Q000378:metabolism*; D017393:RNA Editing / Q000502:physiology*; D012326:RNA Splicing / Q000502:physiology*; D012331:RNA, Fungal / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D000077278:RNA, Mitochondrial; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D024101:Mitochondrial Proteins; D012331:RNA, Fungal; D012333:RNA, Messenger; D000077278:RNA, Mitochondrial; D029701:Saccharomyces cerevisiae Proteins; D012313:RNA; D000604:Amino Acyl-tRNA Synthetases