title: [MiRNA-146a promotes proliferation and migration of rat vascular smooth muscle cells in vitro in a nuclear factor-κB-dependent manner].
abstract: OBJECTIVE
  [corrected] To understand the role of miRNA-146a in the proliferation and migration of primarily cultured rat vascular smooth muscle cells (VSMCs) and investigate the mechanisms.
  
  
  METHODS
  Primarily cultured rat VSMCs were transfected with a synthesized miRNA-146 inhibitor, a scramble sequence or PBS via Lipofectamine2000. Cell counting kit 8 (CCK8) and transwell assay were employed to assess the proliferation and migration of the transfected cells, and the expressions of nuclear factor-κB p65 (NF-κBp65) and proliferation cell nuclear antigen (PCNA) were detected using Western blotting.
  
  
  RESULTS
  A 48-h transfection of the VSMCs with miRNA-146 inhibitor caused significantly lowered miRNA-146a expression as compared with that in VSMCs transfected with the scramble sequence or PBS (P<0.01), resulting also in lowered proliferative and migration ability of the cells (P<0.01). The expression levels of NF-κBp65 and PCNA were remarkably lower in cells transfected with miRNA-146 inhibitor than in the cells in the other two groups (P<0.05).
  
  
  CONCLUSIONS
  miRNA-146a is capable of promoting the proliferation and migration of rat VMSCs probably by enhancing the expression of NF-κBp65.
authors.affiliation: Department of Cardiology, Second Clinical Medical College of Jinan University, Shenzhen, China. xw1012@sina.com
authors.forename: Wei
authors.initials: W
authors.lastname: Xiong
authors.forename: Shaohong
authors.initials: S
authors.lastname: Dong
authors.forename: Jianhui
authors.initials: J
authors.lastname: Yuan
authors.forename: Jianghua
authors.initials: J
authors.lastname: Li
authors.forename: Jianjun
authors.initials: J
authors.lastname: Liu
authors.forename: Xinyun
authors.initials: X
authors.lastname: Xu
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D008297:Male; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics*; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D032389:Myocytes, Smooth Muscle / Q000187:drug effects* / Q000378:metabolism; D061251:Primary Cell Culture; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D051996:Transcription Factor RelA / Q000235:genetics / Q000378:metabolism; D014162:Transfection
chemical_list: C542542:MIRN146 microRNA, human; D035683:MicroRNAs; D018809:Proliferating Cell Nuclear Antigen; C498145:Rela protein, rat; D051996:Transcription Factor RelA