title: Modulation of biogenic amines content by poly(propylene imine) dendrimers in rats.
abstract: Biogenic amines and polyamines participate in all vital organism functions, their levels being important function determinants. Studies were performed to check whether repeated administration of poly(propylene imine) (PPI) dendrimers, synthetic macromolecules with diaminobutane core, and peripheral primary amine groups, may influence the endogenous level of amines, as represented by the two of them: spermidine, a natural derivative of diaminobutane, and histamine. The experiment was carried out on Wistar rats. Fourth generation PPI dendrimer, as well as maltotriose-modified fourth generation PPI dendrimers with (a) cationic open sugar shell and (b) neutral dense sugar shell that possess a higher biocompatibility, was used. Applying the combination of column chromatography on Cellex P and spectrofluorimetric assays of o-phthaldialdehyde, the final amine condensation products were employed to analyze tissue spermidine and histamine outside the central nervous system. Furthermore, radioenzymatic assay was used to measure histamine levels in the brain. The obtained results indicate that in some tissues, the endogenous concentrations of histamine and spermidine may be affected by dendrimers depending on their dose and type of dendrimers.
authors.affiliation: Department of General Biophysics, University of Lodz, 141/143 Pomorska St., 90-236 Lodz, Poland. ciepluch@biol.uni.lodz.pl
authors.forename: Karol
authors.initials: K
authors.lastname: Ciepluch
authors.forename: Barbara
authors.initials: B
authors.lastname: Ziemba
authors.forename: Anna
authors.initials: A
authors.lastname: Janaszewska
authors.forename: Dietmar
authors.initials: D
authors.lastname: Appelhans
authors.forename: Barbara
authors.initials: B
authors.lastname: Klajnert
authors.forename: Maria
authors.initials: M
authors.lastname: Bryszewska
authors.forename: Wiesława Agnieszka
authors.initials: WA
authors.lastname: Fogel
mesh_terms: D000818:Animals; D050091:Dendrimers / Q000494:pharmacology*; D006632:Histamine / Q000378:metabolism*; D008297:Male; D009928:Organ Specificity; D011126:Polypropylenes / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013095:Spermidine / Q000378:metabolism*
chemical_list: D050091:Dendrimers; D011126:Polypropylenes; C443641:poly(propyleneimine); D006632:Histamine; D013095:Spermidine