title: An electrochemical method for functionalization of a 316L stainless steel surface being used as a stent in coronary surgery: irreversible immobilization of fibronectin for the enhancement of endothelial cell attachment.
abstract: An electrochemistry-based method for the formation of functionalized alkanethiol layers on a 316L stainless steel surface was developed. The method was efficient in forming a very stable, irreversibly-attached COOH-terminated (mercaptoundecanoic acid) surface layer. This layer was used as a 'linker' to immobilize the extracellular matrix protein fibronectin to the 316L stainless steel surface. Fibronectin was irreversibly attached to the surface and, unlike physisorbed fibronectin, resisted detachment more in aggressive 0.1 M NaOH under sonication. The fibronectin-modified 316L stainless steel surface was more biocompatible towards attachment of endothelial cells than a bare (unmodified) 316L stainless steel surface, yielding a 25% improvement in cell density.
authors.affiliation: Department of Chemical Engineering, McGill University, Montreal, QC, Canada.
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Harvey
authors.forename: Andreas
authors.initials: A
authors.lastname: Bergdahl
authors.forename: Hesam
authors.initials: H
authors.lastname: Dadafarin
authors.forename: Li
authors.initials: L
authors.lastname: Ling
authors.forename: Elaine C
authors.initials: EC
authors.lastname: Davis
authors.forename: Sasha
authors.initials: S
authors.lastname: Omanovic
mesh_terms: D002448:Cell Adhesion; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D042783:Endothelial Cells / Q000502:physiology*; D005353:Fibronectins / Q000378:metabolism*; D006801:Humans; D015425:Myocardial Reperfusion / Q000379:methods; D013193:Stainless Steel / Q000737:chemistry*; D015607:Stents
chemical_list: D020099:Coated Materials, Biocompatible; D005353:Fibronectins; D013193:Stainless Steel