title: [Effects and mechanism of drug serum of prepared radix polygoni multiflori on rat osteoblast in vitro].
abstract: OBJECTIVE
  To study the effect of drug serum of prepared Radix Polygoni Multiflori on rat osteoblast (Ob) and its mechanism.
  
  
  METHODS
  The animal serum was prepared by serum pharmacology means. The cells were getting by separating and inducing the SD neonatal rat skull bone. The proliferation and differentiation of Ob were detected by CCK-8, alkaline phosphatase (ALP) activity analysis. And RT-PCR method was used to determine the osteogenesis-related genes expression.
  
  
  RESULTS
  Compared with control group, the groups with drug serum of prepared Radix Polygoni Mutiflori, 10%, 20% and 30% had an effect on promotion the proliferation significantly on Ob (P < 0.01). There was no concentration-related manner among groups. The 5% and 10% drug serum decreased ALP activity at the post-translation phase compared with control group, but higher doses (20% and 30%) did not have the same effect. However, drug serum of PR/MIN increased significantly osteogenesis-related genes (OC, ALP, Cbfalpha1) mRNA expression (P < 0.05).
  
  
  CONCLUSIONS
  The drug serum of prepared Radix Polygoni Multiflori can stimulate osteoblast proliferation in vitro, and its mechanism may be associated with increasing osteogenesis-related genes expression.
authors.affiliation: Zhongshan Health Technology Park Development Co. ,Ltd., Zhongshan 528400, China. fzb@nhtp.gov.cn
authors.forename: You-Lian
authors.initials: YL
authors.lastname: Wu
authors.forename: Bi-Yu
authors.initials: BY
authors.lastname: Yang
authors.forename: Jian-Lin
authors.initials: JL
authors.lastname: Gao
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.forename: Li
authors.initials: L
authors.lastname: Lu
authors.forename: Zhao-Li
authors.initials: ZL
authors.lastname: Zeng
authors.forename: Zhong-Hua
authors.initials: ZH
authors.lastname: Qin
authors.forename: Ru-Bin
authors.initials: RB
authors.lastname: Jiang
authors.forename: Qing-Nan
authors.initials: QN
authors.lastname: Li
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D050660:Core Binding Factors / Q000235:genetics / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry; D019664:Polygonaceae / Q000737:chemistry*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D044967:Serum; D012886:Skull / Q000166:cytology
chemical_list: D050660:Core Binding Factors; D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D015675:Osteocalcin; D000469:Alkaline Phosphatase