title: 17β-Estradiol stimulates the translocation of endogenous estrogen receptor α at the plasma membrane of normal anterior pituitary cells.
abstract: In the present work we aimed at identifying ERα in the plasma membrane of normal anterior pituitary cells and investigated if 17β-estradiol was able to induce their subcellular redistribution. Our results show that about 8% of anterior pituitary cells expressed ERα in the plasma membrane, with the geometrical mean fluorescence intensity being increased after steroid hormone treatment. 17β-Estradiol and the selective ERα agonist PPT induced an increase of ERα expression in the plasma membrane and activated the PKCα/ERK 1/2 pathway in a time-course not compatible with genomic actions, thus supporting the notion of membrane-initiated effects. These findings suggest that 17β-estradiol stimulates the translocation of endogenous ERα to the plasma membrane, consequently modulating this ER pool and leading to cellular biological effects in normal anterior pituitary gland.
authors.affiliation: Centro de Microscopía Electrónica, Universidad Nacional de Córdoba, Haya de la Torre esq, Enrique Barros, Ciudad Universitaria, CP 5000 Córdoba, Argentina. silvina@cmefcm.uncor.edu
authors.forename: Silvina
authors.initials: S
authors.lastname: Gutiérrez
authors.forename: Liliana d V
authors.initials: Ld
authors.lastname: Sosa
authors.forename: Juan P
authors.initials: JP
authors.lastname: Petiti
authors.forename: Jorge H
authors.initials: JH
authors.lastname: Mukdsi
authors.forename: Iván D
authors.initials: ID
authors.lastname: Mascanfroni
authors.forename: Claudia G
authors.initials: CG
authors.lastname: Pellizas
authors.forename: Ana L
authors.initials: AL
authors.lastname: De Paul
authors.forename: María J
authors.initials: MJ
authors.lastname: Cambiasso
authors.forename: Alicia I
authors.initials: AI
authors.lastname: Torres
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured; D004958:Estradiol / Q000494:pharmacology*; D047628:Estrogen Receptor alpha / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000235:genetics / Q000378:metabolism; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D008024:Ligands; D010636:Phenols; D010903:Pituitary Gland, Anterior / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D011493:Protein Kinase C / Q000235:genetics / Q000378:metabolism; D021381:Protein Transport / Q000187:drug effects; D011720:Pyrazoles / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects; D015854:Up-Regulation
chemical_list: D047628:Estrogen Receptor alpha; D008024:Ligands; D010636:Phenols; D011720:Pyrazoles; C486184:4,4',4''-(4-propyl-((1)H)-pyrazole-1,3,5-triyl) tris-phenol; D004958:Estradiol; D011493:Protein Kinase C; D048049:Extracellular Signal-Regulated MAP Kinases