title: Application of nanoLC-ESI-TOF-MS for the metabolomic analysis of phenolic compounds from extra-virgin olive oil in treated colon-cancer cells.
abstract: Crude phenolic extracts (PE) have been obtained from naturally bearing Spanish extra-virgin olive oil (EVOO) showing different polyphenol families such as secoiridoids, phenolic alcohols, lignans, and flavones. EVOO-derived complex phenols (especially from the Arbequina variety olive) have been shown to suppress cell growth of SW480 and HT29 human colon adenocarcinoma cell lines. Inhibition of proliferation by EVOO-PE Arbequina variety extract was accompanied by apoptosis in both colon-cancer-cell lines and a limited G₂M cell-cycle arrest in the case of SW480 cells. The metabolized compounds from EVOO-PE in culture medium and cytoplasm of both cell lines were analyzed using nano-liquid chromatography (nanoLC) coupled with electrospray ionization-time-of-flight-mass spectrometry (ESI-TOF-MS). The results showed many phenolic compounds and their metabolites both in the culture medium as well as in the cytoplasm. The main compounds identified from EVOO-PE were hydroxylated luteolin and decarboxymethyl oleuropein aglycone.
authors.affiliation: Department of Analytical Chemistry, Faculty of Sciences, University of Granada, C/Fuentenueva S/N, 18071 Granada, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Fernández-Arroyo
authors.forename: A
authors.initials: A
authors.lastname: Gómez-Martínez
authors.forename: L
authors.initials: L
authors.lastname: Rocamora-Reverte
authors.forename: R
authors.initials: R
authors.lastname: Quirantes-Piné
authors.forename: A
authors.initials: A
authors.lastname: Segura-Carretero
authors.forename: A
authors.initials: A
authors.lastname: Fernández-Gutiérrez
authors.forename: J A
authors.initials: JA
authors.lastname: Ferragut
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D000972:Antineoplastic Agents, Phytogenic / Q000378:metabolism* / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D001711:Biotransformation; D049109:Cell Proliferation / Q000187:drug effects; D002853:Chromatography, Liquid; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology; D017077:Culture Media, Conditioned / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D003653:Decarboxylation; D059565:G2 Phase Cell Cycle Checkpoints / Q000187:drug effects; D019073:HT29 Cells; D006801:Humans; D006900:Hydroxylation; D057888:Iridoid Glucosides; D039823:Iridoids; D047311:Luteolin / Q000378:metabolism; D055432:Metabolomics / Q000379:methods*; D036103:Nanotechnology; D000069463:Olive Oil; D010636:Phenols / Q000378:metabolism* / Q000494:pharmacology; D010938:Plant Oils / Q000378:metabolism* / Q000494:pharmacology; D011714:Pyrans / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D017077:Culture Media, Conditioned; D057888:Iridoid Glucosides; D039823:Iridoids; D000069463:Olive Oil; D010636:Phenols; D010938:Plant Oils; D011714:Pyrans; C002769:oleuropein; D047311:Luteolin