title: Interrogation of alternative splicing events in duplicated genes during evolution.
abstract: BACKGROUND
  Gene duplication provides resources for developing novel genes and new functions while retaining the original functions. In addition, alternative splicing could increase the complexity of expression at the transcriptome and proteome level without increasing the number of gene copy in the genome. Duplication and alternative splicing are thought to work together to provide the diverse functions or expression patterns for eukaryotes. Previously, it was believed that duplication and alternative splicing were negatively correlated and probably interchangeable.
  
  
  RESULTS
  We look into the relationship between occurrence of alternative splicing and duplication at different time after duplication events. We found duplication and alternative splicing were indeed inversely correlated if only recently duplicated genes were considered, but they became positively correlated when we took those ancient duplications into account. Specifically, for slightly or moderately duplicated genes with gene families containing 2 - 7 paralogs, genes were more likely to evolve alternative splicing and had on average a greater number of alternative splicing isoforms after long-term evolution compared to singleton genes. On the other hand, those large gene families (contain at least 8 paralogs) had a lower proportion of alternative splicing, and fewer alternative splicing isoforms on average even when ancient duplicated genes were taken into consideration. We also found these duplicated genes having alternative splicing were under tighter evolutionary constraints compared to those having no alternative splicing, and had an enrichment of genes that participate in molecular transducer activities.
  
  
  CONCLUSIONS
  We studied the association between occurrences of alternative splicing and gene duplication. Our results implicate that there are key differences in functions and evolutionary constraints among singleton genes or duplicated genes with or without alternative splicing incidences. It implies that the gene duplication and alternative splicing may have different functional significance in the evolution of speciation diversity.
authors.affiliation: Institute of Biomedical Informatics, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Ting-Wen
authors.initials: TW
authors.lastname: Chen
authors.forename: Timothy H
authors.initials: TH
authors.lastname: Wu
authors.forename: Wailap V
authors.initials: WV
authors.lastname: Ng
authors.forename: Wen-Chang
authors.initials: WC
authors.lastname: Lin
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D016208:Databases, Factual; D019143:Evolution, Molecular; D020440:Gene Duplication; D006801:Humans; D051379:Mice; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D057386:Search Engine
chemical_list: D020033:Protein Isoforms; D011506:Proteins