title: Human Duffy blood group alloantigen system influences the measurement of monocyte chemoattractant protein-1 (MCP-1) in serum but not in plasma.
abstract: BACKGROUND
  We explored whether the Asp42Gly polymorphism (rs12075) in the DARC gene represents a confounding factor in the interpretation of monocyte chemoattractant protein-1 (MCP-1) concentration in circulating blood.
  
  
  METHODS
  MCP-1 concentration in serum and plasma were measured in 278 healthy Caucasian participants who are representative of our geographic area. The rs12075 genotype distribution was also assessed in this population.
  
  
  RESULTS
  Plasma MCP-1 concentration did not vary among the rs12075 polymorphism derived genotypes [in pg/mL, AA: 171.9 (100.2 - 287.2), AG: 178.9 (105.1 - 326.4) and GG: 173.7 (94.4 - 405.7)]. However, there were significant increases in serum MCP-1 related to the presence of the A allele [in pg/mL, AA: 334.6 (180.4 - 756.4), AG: 299.1 (166.1 - 634.9) and GG: 249.1 (149.3 - 578.1)].
  
  
  CONCLUSIONS
  These findings limit the value of circulating MCP-1 as a biomarker and apparently indicate a pathophysiological role for silent chemokine receptors.
authors.affiliation: Centre de Recerca Biomèdica, Universitat Rovira i Virgili, Hospital Universitari de Sant Joan, IISPV, Reus, Spain.
authors.forename: Gerard
authors.initials: G
authors.lastname: Aragonès
authors.forename: Amaia
authors.initials: A
authors.lastname: Ercilla
authors.forename: María
authors.initials: M
authors.lastname: Barreda
authors.forename: Anna
authors.initials: A
authors.lastname: Rull
authors.forename: Raúl
authors.initials: R
authors.lastname: Beltrán-Debón
authors.forename: Esther
authors.initials: E
authors.lastname: Rodríguez-Gallego
authors.forename: Carlos
authors.initials: C
authors.lastname: Alonso-Villaverde
authors.forename: Jordi
authors.initials: J
authors.lastname: Camps
authors.forename: Jorge
authors.initials: J
authors.lastname: Joven
mesh_terms: D018932:Chemokine CCL2 / Q000097:blood*; D004375:Duffy Blood-Group System / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D010949:Plasma; D020641:Polymorphism, Single Nucleotide; D011956:Receptors, Cell Surface / Q000235:genetics; D044967:Serum
chemical_list: C473745:ACKR1 protein, human; C111835:CCL2 protein, human; D018932:Chemokine CCL2; D004375:Duffy Blood-Group System; D011956:Receptors, Cell Surface