title: (1)H, (13)C, and (15)N backbone chemical shift assignments of StAR-related lipid transfer domain protein 5 (STARD5).
abstract: Steroidogenic acute regulatory (StAR)-related lipid transfer proteins possess a START (steroidogenic acute regulatory-related lipid transfer) domain. START domains are conserved protein modules involved in the non-vesicular intracellular transport of lipids and cholesterol in mammals. Fifteen mammalian proteins, divided in five subfamilies, are reported to possess a START domain. Members of the STARD4 subfamily, i.e. STARD4, 5 and 6 are essentially single START domains and are thought to be involved in the intracellular transport of cholesterol. No structure of a cholesterol-bound START domain from this family has been resolved yet. The determination of the structure of such a complex would contribute to a better understanding of the mechanism of ligand binding and transport by START domains, two unresolved aspects of their structural biology. In this context, we have undertaken the structure determination of a ligand-bound form of STARD5 by NMR. Here, we report the (1)H, (13)C and (15)N backbone resonance assignments of the ligand-free STARD5.
authors.affiliation: Département de Biochimie, Faculté de médecine et des sciences de la santé, Université de Sherbrooke, Sherbrooke, QC, J1H 5N4, Canada.
authors.forename: Aurélien
authors.initials: A
authors.lastname: Lorin
authors.forename: Danny
authors.initials: D
authors.lastname: Létourneau
authors.forename: Andrée
authors.initials: A
authors.lastname: Lefebvre
authors.forename: Jean-Guy
authors.initials: JG
authors.lastname: LeHoux
authors.forename: Pierre
authors.initials: P
authors.lastname: Lavigne
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport; D001665:Binding Sites; D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism; D008024:Ligands; D019906:Nuclear Magnetic Resonance, Biomolecular; D017434:Protein Structure, Tertiary
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D002352:Carrier Proteins; D008024:Ligands; C456546:STARD5 protein, human