title: Purification and characterization of two new allergens from the venom of Vespa magnifica.
abstract: Due to poor diagnostic facilities and a lack of medical alertness, allergy to Vespa wasps may be underestimated. Few allergens have been identified from Vespa wasps.Possible native allergen proteins were purified from the wasp venoms (WV) (Vespa magnifica Smith) by gel filtration, ion exchange chromatography, respectively. Their sequences were determined by Edman degradation and cDNA cloning. Their allergenicities were assayed by enzyme-linked immunosorbent assay inhibition tests (ELISA-IT), immunoblots, and skin prick tests (SPTs). Their cross allergencities with Tab y 2 and Tab y 5 purified from the horsefly (Tabanus yao Macquart) were also determined. Two native allergens were identified from the WV, respectively. They are a 25-KDa antigen 5 protein (Ag5) (Vesp ma 5) and a 35-KDa hyaluronidase (Vesp ma 2). They represented major allergens in Vespa magnifica by immunoblots and SPTs. ELISA inhibition of pooled sera IgE reactivity to both the WV and the horsefly salivary gland extracts (HSGE) using four purified allergens (Vesp ma 2, Vesp ma 5 and previously purified Tab y 2 and Tab y 5) was significant. Their cross allergenicities were confirmed by ELISA-IT, immunoblots, and SPTs. They represented the cross reactive allergens from wasp and horsefly and proved the so called wasp-horsefly syndrome.
authors.affiliation: Biotoxin Units of Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China.
authors.forename: Su
authors.initials: S
authors.lastname: An
authors.forename: Lingling
authors.initials: L
authors.lastname: Chen
authors.forename: Ji-Fu
authors.initials: JF
authors.lastname: Wei
authors.forename: Xuening
authors.initials: X
authors.lastname: Yang
authors.forename: Dongying
authors.initials: D
authors.lastname: Ma
authors.forename: Xuemei
authors.initials: X
authors.lastname: Xu
authors.forename: Xueqing
authors.initials: X
authors.lastname: Xu
authors.forename: Shaoheng
authors.initials: S
authors.lastname: He
authors.forename: Jia
authors.initials: J
authors.lastname: Lu
authors.forename: Ren
authors.initials: R
authors.lastname: Lai
mesh_terms: D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000737:chemistry* / Q000302:isolation & purification; D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology; D002648:Child; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange / Q000379:methods; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006031:Glycosylation; D006801:Humans; D007073:Immunoglobulin E / Q000737:chemistry; D008297:Male; D008875:Middle Aged; D014862:Wasp Venoms / Q000378:metabolism*; D014863:Wasps / Q000502:physiology*
chemical_list: D000485:Allergens; D000925:Anticoagulants; D014862:Wasp Venoms; D007073:Immunoglobulin E