title: The role of radix hedysari polysaccharide on the human umbilical vein endothelial cells (HUVECs) induced by high glucose.
abstract: BACKGROUND
  Diabetes mellitus can cause a wide variety of vascular complications and it is one of the major risk factors for cardiovascular diseases (CVD). High glucose can induce vascular endothelial cell apoptosis. In this study, we investigated the effect of radix hedysari polysaccharide (HPS) on the depression of apoptosis of human umbilical vein endothelial cells (HUVECs) induced by high glucose.
  
  
  METHODS
  HUVECs were treated with media containing 30 mM glucose in the presence or absence of vitamin C or HPS. The level of intracellular reactive oxygen species (ROS) and apoptosis of HUVECs was measured with flow cytometry. Expression of c-Jun NH(2)-terminal kinase (JNK) and caspase-3 were testified by real-time quantitative RT-PCR and immunofluorescence.
  
  
  RESULTS
  High glucose was capable of eliciting the overexpression of JNK during the treatment procedure. Moreover, we found that the caspase-3 became overexpressed in apoptosis induced by high glucose; HPS could inhibit apoptosis under high glucose and suppress the generation of ROS and the overexpression of JNK and caspase-3. The effect of HPS on ROS quenching, inhibition of JNK and caspase-3 overexpression at the concentration of 100 μg/ml was similar to that of vitamin C at the concentration of 100 μM.
  
  
  CONCLUSIONS
  The findings of the present study may suggest that HPS play a protection role on HUVECs against apoptosis induced by high glucose.
authors.affiliation: Department of Endocrinology, People's Hospital of Gansu Province, Lanzhou, Gansu 730000, China. liujingwelcome@126.com
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Deng
authors.forename: Lei
authors.initials: L
authors.lastname: Fan
authors.forename: Limin
authors.initials: L
authors.lastname: Tian
authors.forename: Liying
authors.initials: L
authors.lastname: Jin
authors.forename: Zhisheng
authors.initials: Z
authors.lastname: Jin
authors.forename: Qian
authors.initials: Q
authors.lastname: Guo
authors.forename: Yanjia
authors.initials: Y
authors.lastname: Xu
authors.forename: Ning
authors.initials: N
authors.lastname: Li
mesh_terms: D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D027885:Astragalus propinquus; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D019610:Cytoprotection / Q000187:drug effects; D004347:Drug Interactions; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D005947:Glucose / Q000633:toxicity*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; C027492:Huang Qi; D048031:JNK Mitogen-Activated Protein Kinases; C505407:CASP3 protein, human; D053148:Caspase 3; D005947:Glucose