title: Dissemination of pHK01-like incompatibility group IncFII plasmids encoding CTX-M-14 in Escherichia coli from human and animal sources.
abstract: Few studies have compared CTX-M encoding plasmids identified in different ecological sources. This study aimed to analyze and compare the molecular epidemiology of plasmids encoding CTX-M-14 among strains from humans and animals. The CTX-M-14 encoding plasmids in 160 Escherichia coli isolates from animal faecal (14 pigs, 16 chickens, 12 cats, 8 cattle, 5 dogs and 3 rodents), human faecal (45 adults and 20 children) and human urine (37 adults) sources in 2002-2010 were characterized by molecular methods. The replicon types of the CTX-M-14 encoding plasmids were IncFII (n=61), I1-Iγ (n=24), other F types (n=23), B/O (n=10), K (n=6), N (n=3), A/C (n=1), HI1 (n=1), HI2 (n=1) and nontypeable (n=30). The genetic environment, ISEcp1 -bla(CTX-M-14) - IS903 was found in 89.7% (52/58), 87.7% (57/65) and 86.5% (32/37) of the animal faecal, human faecal and human urine isolates, respectively. Subtyping of the 61 IncFII incompatibility group plasmids by replicon sequence typing, plasmid PCR-restriction fragment length polymorphism and marker genes (yac, malB, eitA/eitC and parB/A) profiles showed that 31% (18/58), 30.6% (20/65) and 37.8% (14/37) of the plasmids originating from animal faecal, human faecal and human urine isolates, respectively, were pHK01-like. These 52 pHK01-like plasmids originated from diverse human (20 faecal isolates from 2002, 2007 to 2008, 14 urinary isolates from 2004) and animal (all faecal, 1 cattle, 1 chicken, 5 pigs, 9 cats, 1 dog, 1 rodent from 2008 to 2010) sources. In conclusion, this study highlights the importance of the IncFII group, pHK01-like plasmids in the dissemination of CTX-M-14 among isolates from diverse sources.
authors.affiliation: Department of Microbiology, University of Hong Kong, Pokfulam Road, Pokfulam, Hong Kong, China. plho@hkucc.hku.hk
authors.forename: P L
authors.initials: PL
authors.lastname: Ho
authors.forename: W U
authors.initials: WU
authors.lastname: Lo
authors.forename: M K
authors.initials: MK
authors.lastname: Yeung
authors.forename: Zhen
authors.initials: Z
authors.lastname: Li
authors.forename: Jane
authors.initials: J
authors.lastname: Chan
authors.forename: K H
authors.initials: KH
authors.lastname: Chow
authors.forename: W C
authors.initials: WC
authors.lastname: Yam
authors.forename: Amy H Y
authors.initials: AH
authors.lastname: Tong
authors.forename: Jessie Y J
authors.initials: JY
authors.lastname: Bao
authors.forename: C H
authors.initials: CH
authors.lastname: Lin
authors.forename: Si
authors.initials: S
authors.lastname: Lok
authors.forename: Susan S
authors.initials: SS
authors.lastname: Chiu
mesh_terms: D000328:Adult; D000818:Animals; D002648:Child; D004926:Escherichia coli / Q000235:genetics* / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology* / Q000662:veterinary*; D029968:Escherichia coli Proteins / Q000235:genetics; D005243:Feces / Q000382:microbiology; D005260:Female; D006801:Humans; D017720:Molecular Epidemiology; D010957:Plasmids; D014556:Urine / Q000382:microbiology; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D029968:Escherichia coli Proteins; C456080:beta-lactamase CTX-M-14; D001618:beta-Lactamases