title: [Effects of extracellular matrix produced in vitro on growth and differentiation of MC3T3-E1 cells].
abstract: Extracellular matrix (ECM) keeps cell's shape, protects and nourishes cells; it plays a great role in cell proliferation and differentiation. Therefore, ECM is very important in cell and tissue engineering. In this study, after primary mouse osteoblasts and fibroblasts maintained at confluence in vitro were removed, their ECM coated on cell culture plate was prepared, and bone morphogenetic proteins 2 (BMP-2) was detected in the osteoblasts ECM. MC3T3-E1 preosteoblasts cells were seeded on cell culture plates covered with fibroblasts ECM and osteoblasts ECM respectively. The proliferative activity of the cells cultured on fibroblasts ECM was higher than that on osteoblasts ECM and the control group. The alkaline phosphatase activity, relative protein levels of BMP-2 and osteopontin, secreted calcium of the cells cultured on osteoblasts ECM were all the highest. The results indicate that the two different ECMs produced in vitro had different bioactivities, the fibroblasts ECM coated on cell culture plates could accelerate MC3T3-E1 cells proliferation, and the osteoblasts ECM could promote cells osteogenic differentiation.
authors.affiliation: Tianjin Institute of Medical Equipment, Tianjin 300161, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Guo
authors.forename: Lu
authors.initials: L
authors.lastname: Liu
authors.forename: Qingxin
authors.initials: Q
authors.lastname: Hao
authors.forename: Ruixin
authors.initials: R
authors.lastname: Li
authors.forename: Xizheng
authors.initials: X
authors.lastname: Zhang
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.forename: Bo
authors.initials: B
authors.lastname: Ning
mesh_terms: D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D010006:Osteoblasts / Q000166:cytology*; D053495:Osteopontin / Q000378:metabolism
chemical_list: C525719:Bmp2 protein, mouse; D055396:Bone Morphogenetic Protein 2; D053495:Osteopontin; D000469:Alkaline Phosphatase; D002118:Calcium