title: Tight controlled expression and secretion of Lactobacillus brevis SlpA in Lactococcus lactis.
abstract: Prokaryotes commonly present outer cell wall structures composed of a crystalline array of proteinaceous subunits, known as surface layers (S-layers). The ORF encoding the S-layer protein (SlpA) of Lactobacillus brevis was cloned into Lactococcus lactis under the transcriptional control of the xylose-inducible expression system (XIES). SlpA was secreted into the extracellular medium, as determined by immunoblotting, and assays on the kinetics of SlpA production revealed that repression of the system with glucose did not require the depletion of xylose from the medium that allows transitory ORF expression. The successful use of XIES to express S-layer proteins in the versatile and generally recognized as safe species L. lactis opens new possibilities for an efficient production and isolation of SlpA S-layer protein for its various applications in biotechnology and importantly as an antigen-carrying vehicle.
authors.affiliation: Laboratorio de Microbiología Molecular, Departamento de Cienciay Tecnología, Universidad Nacional de Quilmes, Bernal, Argentina.
authors.forename: Axel
authors.initials: A
authors.lastname: Hollmann
authors.forename: Mariano
authors.initials: M
authors.lastname: Saviello
authors.forename: Lucrecia
authors.initials: L
authors.lastname: Delfederico
authors.forename: Tessália Diniz Luerce
authors.initials: TD
authors.lastname: Saraiva
authors.forename: Debmalya
authors.initials: D
authors.lastname: Barh
authors.forename: Neha
authors.initials: N
authors.lastname: Jain
authors.forename: Sandeep
authors.initials: S
authors.lastname: Tiwari
authors.forename: Sudha
authors.initials: S
authors.lastname: Chandra
authors.forename: Krishnakant
authors.initials: K
authors.lastname: Gupta
authors.forename: Vasudeo
authors.initials: V
authors.lastname: Zambare
authors.forename: Anil
authors.initials: A
authors.lastname: Kumar
authors.forename: Lew
authors.initials: L
authors.lastname: Christopher
authors.forename: Amarendra Narayan
authors.initials: AN
authors.lastname: Misra
authors.forename: Ranjith N
authors.initials: RN
authors.lastname: Kumavath
authors.forename: Vasco
authors.initials: V
authors.lastname: Azevedo
authors.forename: Liliana
authors.initials: L
authors.lastname: Semorile
authors.forename: Anderson
authors.initials: A
authors.lastname: Miyoshi
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D003001:Cloning, Molecular; D003470:Culture Media / Q000737:chemistry; D015870:Gene Expression; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D005947:Glucose / Q000378:metabolism; D052196:Levilactobacillus brevis / Q000235:genetics*; D013294:Lactococcus lactis / Q000235:genetics* / Q000378:metabolism*; D014994:Xylose / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; C070683:surface layer protein A, Bacteria; D014994:Xylose; D005947:Glucose