title: Immune reconstitution to cytomegalovirus following partially matched-related donor transplantation: impact of in vivo T-cell depletion and granulocyte colony-stimulating factor-primed peripheral blood/bone marrow mixed grafts.
abstract: Cytomegalovirus (CMV) infection and delayed immune reconstitution remains a serious obstacle for successful partially matched-related donor transplantation (PMRD). We evaluated 42 patients for the development of CMV-specific CD8+ T lymphocytes (CTL(CMV) ) following granulocyte colony-stimulating factor-primed peripheral blood (PB) and bone marrow (BM) with anti-thymocyte globulin (ATG)-based PMRD. PMRD recipients achieved a high frequency, proliferation capacity, and interferon-γ response of CTL(CMV) at 1 year post transplantation. CTL(CMV) with the central memory CD45RO+CD62L+ cell phenotype expanded in PB and BM-resident CTL(CMV) displayed distinct phenotypes when CMV was reactivated. Although the incidence of CMV reactivation was high in PMRD patients (87.67%), only 11.90% of them developed CMV disease. In conclusion, after PMRD using mixed grafts with ATG-based conditioning, immune recovery to CMV seems to be early and fast, thereby reducing the incidence of CMV disease.
authors.affiliation: Peking University, People's Hospital, Peking University Institute of Hematology, Peking University, Beijing, China. xiaohua.luo@gmail.com
authors.forename: X-H
authors.initials: XH
authors.lastname: Luo
authors.forename: X-J
authors.initials: XJ
authors.lastname: Huang
authors.forename: D
authors.initials: D
authors.lastname: Li
authors.forename: K-Y
authors.initials: KY
authors.lastname: Liu
authors.forename: L-P
authors.initials: LP
authors.lastname: Xu
authors.forename: D-H
authors.initials: DH
authors.lastname: Liu
mesh_terms: D000293:Adolescent; D000328:Adult; D000961:Antilymphocyte Serum / Q000627:therapeutic use; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002648:Child; D003587:Cytomegalovirus / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000276:immunology*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D008212:Lymphocyte Depletion; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D014019:Tissue Donors; D019172:Transplantation Conditioning; D014775:Virus Activation; D055815:Young Adult
chemical_list: D000961:Antilymphocyte Serum; D016179:Granulocyte Colony-Stimulating Factor