title: Adsorption behaviors of recombinant E-cadherin-IgG Fc fusion protein on polystyrene surface.
abstract: Adsorption behaviors of recombinant E-cadherin-IgG Fc (E-cad-Fc) fusion protein and mutated E-cad-Fcs on the polystyrene (PS) surface were investigated using a 27 MHz quartz-crystal microbalance (QCM) and ELISA. The amount of adsorbed E-cad-Fc on PS surface was increased with an increase of E-cad-Fc concentration as a Langmuir-type in a monolayer. Adsorbed E-cad-Fc on PS surface was stable even after washing if calcium ions are absent in the washing solution due to the calcium ion dependence in the adsorption. E-cadherin homophilic adhesion among E-cadherins during adsorption of E-cad-Fc was involved. Deglycosylation of the E-cad in the E-cad-Fc did not affect adsorption of E-cad-Fc on the PS surface although deglycosylation of the E-cad in the E-cad-Fc enhanced cell adhesion compared with E-cad-Fc.
authors.affiliation: Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori-ku, Yokohama, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Sakai
authors.forename: J
authors.initials: J
authors.lastname: Kim
authors.forename: B
authors.initials: B
authors.lastname: Hexig
authors.forename: Y
authors.initials: Y
authors.lastname: Okahata
authors.forename: C S
authors.initials: CS
authors.lastname: Cho
authors.forename: T
authors.initials: T
authors.lastname: Akaike
mesh_terms: D000327:Adsorption; D000818:Animals; D016466:CHO Cells; D015820:Cadherins / Q000737:chemistry* / Q000378:metabolism; D002118:Calcium / Q000737:chemistry; D002448:Cell Adhesion; D006224:Cricetinae; D054278:Embryonal Carcinoma Stem Cells; D004797:Enzyme-Linked Immunosorbent Assay; D007074:Immunoglobulin G / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D009154:Mutation; D011137:Polystyrenes / Q000737:chemistry; D058525:Quartz Crystal Microbalance Techniques; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D023822:Tissue Engineering
chemical_list: D015820:Cadherins; D007074:Immunoglobulin G; D011137:Polystyrenes; D011993:Recombinant Fusion Proteins; D002118:Calcium