title: A molecular and proteomic investigation of proteins rapidly released from triticale pollen upon hydration.
abstract: Analysis of Triticale (×Triticosecale Wittmack cv. AC Alta) mature pollen proteins quickly released upon hydration was performed using two-dimensional gel electrophoresis followed by mass spectrometry. A total of 17 distinct protein families were identified and these included expansins, profilins, and various enzymes, many of which are pollen allergens. The corresponding genes were obtained and expression studies revealed that the majority of these genes were only expressed in developing anthers and pollen. Some genes including glucanase, glutathione peroxidase, glutaredoxin, and a profilin were found to be widely expressed in different reproductive and vegetative tissues. Group 11 pollen allergens, polygalacturonase, and actin depolymerizing factor were characterized for the first time in the Triticeae. This study represents a distinctive combination of proteomic and molecular analyses of the major cereal pollen proteins released upon hydration and therefore at the forefront of pollen-stigma interactions.
authors.affiliation: Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, Ottawa, ON K1A 0C6, Canada.
authors.forename: Mohsin A
authors.initials: MA
authors.lastname: Zaidi
authors.forename: Stephen
authors.initials: S
authors.lastname: O'Leary
authors.forename: Shaobo
authors.initials: S
authors.lastname: Wu
authors.forename: Steve
authors.initials: S
authors.lastname: Gleddie
authors.forename: François
authors.initials: F
authors.lastname: Eudes
authors.forename: André
authors.initials: A
authors.lastname: Laroche
authors.forename: Laurian S
authors.initials: LS
authors.lastname: Robert
mesh_terms: D000485:Allergens / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D015152:Blotting, Northern; D018076:DNA, Complementary / Q000235:genetics; D002523:Edible Grain / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D018506:Gene Expression Regulation, Plant; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009928:Organ Specificity; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011058:Pollen / Q000201:enzymology / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014867:Water / Q000378:metabolism*
chemical_list: D000485:Allergens; D018076:DNA, Complementary; D010940:Plant Proteins; D012333:RNA, Messenger; D014867:Water