title: [Correlation between expression level of functional genes and tanshinones' accumulation in Salvia miltiorrhiza from different areas].
abstract: OBJECTIVE
  To study the relationship between functional gene expression in Salvia miltiorrhiza from different producing areas and active principles, which might provide scientific basis for the gene regulation of tanshinones.
  
  
  METHODS
  The quantitative determination of cryptotanshinone and tanshinone II A was carried out by using HPLC method, expression level of 3 functional genes of SmAACT, SmCMK and SmIPPI were investigated by real-time PCR method.
  
  
  RESULTS
  The content of active principles together with expression level of SmAACT and SmCMK were higher in S. miltiorrhiza from genuine producing areas including Henan and Shanxi, but lower in samples from Beijing which was non-genuine producing area.
  
  
  CONCLUSIONS
  Expression level of SmAACT and SmCMK had close relationships involving tanshinones' accumulation, but the SmIPPI gene had not.
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China.
authors.forename: Beining
authors.initials: B
authors.lastname: Li
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Zhou
authors.forename: Luqi
authors.initials: L
authors.lastname: Huang
authors.forename: Xueyong
authors.initials: X
authors.lastname: Wang
authors.forename: Chunsheng
authors.initials: C
authors.lastname: Liu
mesh_terms: D045784:Abietanes / Q000378:metabolism*; D015870:Gene Expression; D060888:Real-Time Polymerase Chain Reaction; D027561:Salvia miltiorrhiza / Q000235:genetics* / Q000378:metabolism*
chemical_list: D045784:Abietanes; C021751:tanshinone