title: Removing endotoxin from plasmid samples by Triton X-114 isothermal extraction.
abstract: Triton X-114 temperature transition extraction has been considered to be a simple and cost-effective strategy to eliminate endotoxin from plasmid preparations. However, a repeated cooling-heating process may promote the degradation of plasmid DNA. Based on the finding that the cloud point of Triton X-114 solution increases substantially in the presence of small amounts of sodium dodecyl sulfate (SDS) and that electrolytes decrease the cloud point of Triton X-114-SDS solution drastically, we designed a Triton X-114 isothermal extraction method for removing endotoxin from plasmid samples and found that it has the same endotoxin removal efficiency when compared with the temperature transition extraction method.
authors.affiliation: Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi'an, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Ma
authors.forename: Jun
authors.initials: J
authors.lastname: Zhao
authors.forename: Hui-Cong
authors.initials: HC
authors.lastname: Du
authors.forename: Shuang
authors.initials: S
authors.lastname: Tian
authors.forename: Li-Wen
authors.initials: LW
authors.lastname: Li
mesh_terms: D004247:DNA / Q000302:isolation & purification*; D003902:Detergents / Q000737:chemistry; D004731:Endotoxins / Q000302:isolation & purification*; D017830:Octoxynol; D044367:Phase Transition; D010957:Plasmids / Q000302:isolation & purification*; D011092:Polyethylene Glycols / Q000737:chemistry*; D012965:Sodium Chloride / Q000737:chemistry; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013696:Temperature
chemical_list: D003902:Detergents; D004731:Endotoxins; D012967:Sodium Dodecyl Sulfate; D011092:Polyethylene Glycols; D012965:Sodium Chloride; D017830:Octoxynol; D004247:DNA; C010615:Nonidet P-40