title: Development of biodegradable scaffolds based on magnetically guided assembly of magnetic sugar particles.
abstract: Biodegradable scaffolds with controlled pore layout and porosity have great significance in tissue engineering for cell penetration, tissue ingrowth, vascularization, and nutrient delivery. Porogen leaching has been commonly used to control pore size, pore structure and porosity in the scaffold. In this paper we focus on the use/development of two magnetically guided porogen assembly methods using magnetic sugar particles (MSPs) for scaffold fabrication. First, a patterning device is utilized to align MSPs following designed templates. Then a magnetic sheet film is fabricated by mixing poly(vinyl alcohol, PVA) and NdFeB powder for steering the MSPs. After poly(l-lactide-co-ɛ-caprolactone) (PLCL) casting and removal of the sugar template, a scaffold with spherical pores is obtained. The surface and the inner structure of the scaffolds are evaluated using light and electron micrographs showing their interconnection of pores, pore wall morphology and porosity. Single layer scaffolds with the size of 8mm in width and 10mm in length were constructed with controllable pore diameters in the ranges of 105-150 μm, 250-300 μm and 425-500 μm.
authors.affiliation: Dept. of Micro-Nano Systems Engineering, Nagoya University, Japan. huchengz@gmail.com
authors.forename: Chengzhi
authors.initials: C
authors.lastname: Hu
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Uchida
authors.forename: Carlos
authors.initials: C
authors.lastname: Tercero
authors.forename: Seiichi
authors.initials: S
authors.lastname: Ikeda
authors.forename: Katsutoshi
authors.initials: K
authors.lastname: Ooe
authors.forename: Toshio
authors.initials: T
authors.lastname: Fukuda
authors.forename: Fumihito
authors.initials: F
authors.lastname: Arai
authors.forename: Makoto
authors.initials: M
authors.lastname: Negoro
authors.forename: Guiryong
authors.initials: G
authors.lastname: Kwon
mesh_terms: D001672:Biocompatible Materials; D002470:Cell Survival; D055119:Elastic Modulus; D004867:Equipment Design; D005632:Fructose / Q000737:chemistry*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D059346:Magnets / Q000737:chemistry*; D010316:Particle Size; D011091:Polyesters / Q000737:chemistry; D011142:Polyvinyl Alcohol / Q000737:chemistry; D016062:Porosity; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; D005632:Fructose; C062968:lactide-caprolactone copolymer; D011142:Polyvinyl Alcohol