title: Long-term following-up of viability of spleen autotransplants in the Beagle canine model.
abstract: OBJECTIVE
  To examine the possible late complications of splenectomy or spleen autotransplantation in large laboratory animal model, in which we need non-invasive or minimal-invasive methods for long-term monitoring of the experimental animals.
  
  
  METHODS
  Experimental groups of beagle dogs were: non-operated control, sham-operated control, splenectomy, spleen autotransplantation with 5 or 10 spleen-chips taken into the greater omentum (Furka's technique). Prior to operations, on the 1(st) postoperative week, monthly till the 6(th) as well as in the 9(th) and 12(th) month, hemorheological examinations were performed. In postoperative 12(th) month colloid scintigraphy and diagnostic laparoscopy were carried out. At the end of the investigation comparative morphological examinations were performed, too.
  
  
  RESULTS
  From the 4(th)-5(th) postoperative month filtration function of spleen-autotransplants showed particular restoration compared to splenectomy group. However, the functional results did not reach the values of the control or sham-operated groups. Sham-operated control's scintigraphy nicely showed activity in the spleen. In spleen autotransplantation-groups scintigraphy indicated well the activity of spleen-chips. During diagnostic laparoscopy spleen-chips with their blood supply were found. Histologically, the structure of spleen-autotransplants was similar to normal splenic tissue.
  
  
  CONCLUSIONS
  The autotransplants are regenerated, their functions have been partly restored, and thus spleen autotransplantation may prevent the possible complications of splenectomy. These parameters and the presented investigative protocol are suitable for long-term following-up of viability of the spleen-autotransplants.
authors.affiliation: Department of Operative Techniques and Surgical Research, Medical and Health Science Center, University of Debrecen, Hungary. sajtos.erika@gmail.com
authors.forename: Erika
authors.initials: E
authors.lastname: Sajtos
authors.forename: Anita
authors.initials: A
authors.lastname: Balint
authors.forename: Endre
authors.initials: E
authors.lastname: Brath
authors.forename: Norbert
authors.initials: N
authors.lastname: Nemeth
authors.forename: Katalin
authors.initials: K
authors.lastname: Peto
authors.forename: Judit
authors.initials: J
authors.lastname: Kovacs
authors.forename: Laszlo
authors.initials: L
authors.lastname: Galuska
authors.forename: Jozsef
authors.initials: J
authors.lastname: Varga
authors.forename: Zoltan
authors.initials: Z
authors.lastname: Fodor
authors.forename: Istvan
authors.initials: I
authors.lastname: Furka
authors.forename: Iren
authors.initials: I
authors.lastname: Miko
mesh_terms: D000818:Animals; D003102:Colloids; D004285:Dogs; D005240:Feasibility Studies; D005260:Female; D010535:Laparoscopy; D008297:Male; D023421:Models, Animal; D011184:Postoperative Period; D011877:Radionuclide Imaging; D012038:Regeneration; D013154:Spleen / Q000000981:diagnostic imaging / Q000502:physiology / Q000637:transplantation*; D013156:Splenectomy / Q000009:adverse effects*; D013997:Time Factors; D014182:Transplantation, Autologous / Q000009:adverse effects / Q000379:methods; D016896:Treatment Outcome
chemical_list: D003102:Colloids