title: Ultrasensitive and selective detection of nicotinamide adenine dinucleotide by target-triggered ligation-rolling circle amplification.
abstract: An ultrasensitive fluorescence assay for nicotinamide adenine dinucleotide (NAD(+)) was developed by target-triggered ligation-rolling circle amplification (L-RCA). This novel approach can detect as low as 1 pM NAD(+), much lower than those of previously reported biosensors, and exhibits high discrimination ability even against 200 times excess of NAD(+) analogs.
authors.affiliation: Key Laboratory of Biomedical Information Engineering of Education Ministry, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, Shaanxi 710049, P. R. China. yxzhao@mail.xjtu.edu.cn
authors.forename: Yongxi
authors.initials: Y
authors.lastname: Zhao
authors.forename: Lin
authors.initials: L
authors.lastname: Qi
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Dong
authors.forename: Yu
authors.initials: Y
authors.lastname: Kong
authors.forename: Yayan
authors.initials: Y
authors.lastname: Wu
authors.forename: Chunhai
authors.initials: C
authors.lastname: Fan
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry*; D001681:Biological Assay; D015374:Biosensing Techniques; D011088:DNA Ligases / Q000737:chemistry*; D017931:DNA Primers / Q000737:chemistry; D004270:DNA, Circular / Q000737:chemistry; D004926:Escherichia coli / Q000737:chemistry; D005453:Fluorescence; D009243:NAD / Q000032:analysis*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
chemical_list: D052157:Aptamers, Nucleotide; D001426:Bacterial Proteins; D017931:DNA Primers; D004270:DNA, Circular; D009243:NAD; D011088:DNA Ligases; C120640:DNA ligase (NAD)