title: In vitro CMV-infection model in fresh and glycerolized skin graft.
abstract: Viral infections, especially cytomegalovirus (CMV), are a cause of death in burned patients. Aim of this study was to perform an in vitro CMV-infection model comparing fresh and glycerol-treated fibroblasts and keratinocytes. Cells were plated in plates for the two conditions. Each plate was set up with CMV dilutions. Immunofluorescence and real time PCR assays were performed. The assays were negative in both fresh and glycerolized keratinocytes. For fibroblasts, CMV-DNA was positive in both conditions and immunofluorescence test only in fresh cells. Glycerol at 85% confirms its strong virucidal effect as reported also for other viruses.
authors.affiliation: University Hospital San Giovanni Battista di Torino, Turin, Italy. sara.astegiano@unito.it
authors.forename: Sara
authors.initials: S
authors.lastname: Astegiano
authors.forename: Daniela
authors.initials: D
authors.lastname: Alotto
authors.forename: Carlotta
authors.initials: C
authors.lastname: Castagnoli
authors.forename: Franca
authors.initials: F
authors.lastname: Sinesi
authors.forename: Maurizio
authors.initials: M
authors.lastname: Stella
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Bergallo
authors.forename: Rossana
authors.initials: R
authors.lastname: Cavallo
mesh_terms: D003451:Cryoprotective Agents / Q000494:pharmacology / Q000633:toxicity; D003587:Cytomegalovirus / Q000187:drug effects / Q000235:genetics; D003586:Cytomegalovirus Infections / Q000821:virology*; D005347:Fibroblasts / Q000378:metabolism / Q000821:virology; D005990:Glycerol / Q000494:pharmacology / Q000633:toxicity; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism / Q000821:virology; D061251:Primary Cell Culture; D012867:Skin / Q000187:drug effects / Q000378:metabolism / Q000821:virology; D016038:Skin Transplantation; D046509:Tissue Culture Techniques; D014184:Transplantation, Homologous; D019737:Transplants / Q000821:virology*
chemical_list: D003451:Cryoprotective Agents; D005990:Glycerol