title: Expression of SDF-1α and leptin, and their effect on expression of angiogenic factors in mouse ovaries.
abstract: OBJECTIVE
  Ovarian angiogenesis plays an important role in folliculogenesis. However, little is known about the expression of angiogenic factors during follicular development according to female age. Stromal cell derived factor-1α (SDF-1α) plays a role in granulosa cell survival and embryo quality as an angiogenic chemokine. Leptin is also involved in folliculogenesis and angiogenesis. This study examined expression of SDF-1α and leptin, and their effects on the expression of angiogenic factors in the ovary during follicular development according to female age.
  
  
  METHODS
  Ovaries were collected from C57BL mice of two age groups (6-9 weeks and 24-26 weeks) at 6, 12, 24, and 48 hours after 5 IU pregnant mare's serum gonadotropin (PMSG) injection. The expression of ovarian SDF-1α and leptin mRNA was evaluated by RT-PCR. In the organ culture experiment, the ovaries were cultured in transwell permeable supports with Waymouth's medium treated with various doses of SDF-1α (50-200 ng/mL) or leptin (0.01-1 µg/mL) for 7 days. Then, mRNA expression of vascular endothelial growth factor (VEGF), endothelial nitric oxide synthase (eNOS), and visfatin were examined in the cultured ovaries.
  
  
  RESULTS
  Expression of SDF-1α and leptin in the ovary was significantly lower in the aged mouse group compared to the young mouse group (p<0.05). Expression of these two factors increased with follicular development after PMSG administration. SDF-1α treatment stimulated visfatin expression in a dose-dependent manner, while leptin treatment significantly increased eNOS expression.
  
  
  CONCLUSIONS
  These results suggest that decrease of ovarian SDF-1α and leptin expression may be associated with aging-related reduction of ovarian function. SDF-1α and leptin may play a role in follicular development by regulating the expression of angiogenic factors in mouse ovaries.
authors.affiliation: Center for Reproductive Medicine, Good Moonhwa Hospital, Busan, Korea.
authors.forename: Min Jung
authors.initials: MJ
authors.lastname: Park
authors.forename: Sea Hee
authors.initials: SH
authors.lastname: Park
authors.forename: Su Kyung
authors.initials: SK
authors.lastname: Lee
authors.forename: Sung Eun
authors.initials: SE
authors.lastname: Moon
authors.forename: Hwa Sook
authors.initials: HS
authors.lastname: Moon
authors.forename: Bo Sun
authors.initials: BS
authors.lastname: Joo