title: LC-MS data processing with MAVEN: a metabolomic analysis and visualization engine.
abstract: MAVEN is an open-source software program for interactive processing of LC-MS-based metabolomics data. MAVEN enables rapid and reliable metabolite quantitation from multiple reaction monitoring data or high-resolution full-scan mass spectrometry data. It automatically detects and reports peak intensities for isotope-labeled metabolites. Menu-driven, click-based navigation allows visualization of raw and analyzed data. Here we provide a User Guide for MAVEN. Step-by-step instructions are provided for data import, peak alignment across samples, identification of metabolites that differ strongly between biological conditions, quantitation and visualization of isotope-labeling patterns, and export of tables of metabolite-specific peak intensities. Together, these instructions describe a workflow that allows efficient processing of raw LC-MS data into a form ready for biological analysis.
authors.affiliation: Department of Chemistry and Integrative Genomics, Carl Icahn Laboratory, Princeton, New Jersey, USA.
authors.forename: Michelle F
authors.initials: MF
authors.lastname: Clasquin
authors.forename: Eugene
authors.initials: E
authors.lastname: Melamud
authors.forename: Joshua D
authors.initials: JD
authors.lastname: Rabinowitz
mesh_terms: D000465:Algorithms; D002853:Chromatography, Liquid / Q000379:methods; D016208:Databases, Factual; D007553:Isotope Labeling; D013058:Mass Spectrometry / Q000379:methods; D055432:Metabolomics / Q000379:methods*; D012984:Software