title: Direct electrochemistry and electrocatalysis of hemoglobin immobilized on polyacrylamide-P123 film modified glassy carbon electrode.
abstract: A novel organic mesoporous material, polyacrylamide-P123 (PAM-P123) composite film, was used to incorporate hemoglobin (Hb) onto the surface of glassy carbon (GC) electrode for studying the direct electron transfer of Hb and fabricating a sensitive biosensor of H(2)O(2). Compared with inorganic mesoporous material, the PAM-P123 composite film has better film-forming property, which is particularly useful for preparing modified electrodes on various substrates for voltammetric measurements. The cyclic voltammetry of Nafion/Hb/PAM-P123/GC modified electrode showed a couple of well-defined and quasi-reversible redox peaks at about 0.317 V (vs. SCE) in pH 7.0 phosphate buffer solution. The Nafion/Hb/PAM-P123/GC modified electrode showed a fast amperometric response and good stability for the detection of hydrogen peroxide. The results indicate that the PAM-P123 film has a promising potential in fabricating third-generation electrochemical biosensors.
authors.affiliation: Key Laboratory for Advanced Materials and Department of Chemistry, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Jinxia
authors.initials: J
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Tang
authors.forename: Lihui
authors.initials: L
authors.lastname: Zhou
authors.forename: Xia
authors.initials: X
authors.lastname: Han
authors.forename: Honglai
authors.initials: H
authors.lastname: Liu
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D002244:Carbon; D055664:Electrochemical Techniques / Q000295:instrumentation* / Q000379:methods*; D004566:Electrodes; D004579:Electron Transport; D005465:Fluorocarbon Polymers / Q000737:chemistry; D006454:Hemoglobins / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D055767:Immobilized Proteins / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D010084:Oxidation-Reduction; D013056:Spectrophotometry, Ultraviolet; D014961:X-Ray Diffraction; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D002244:Carbon; D005465:Fluorocarbon Polymers; D006454:Hemoglobins; D006861:Hydrogen Peroxide; D055767:Immobilized Proteins; D000099166:Polyacrylamides; C040402:perfluorosulfonic acid