title: [Comparison of different deafening strategies based on ototoxic drugs on mouse animals model].
abstract: OBJECTIVE
  To compare safety, reliability and usefulness of two deafening protocols on animal mouse model, based on aminoglycosides exposure
  
  
  METHODS
  Adults mice, Bulb/C, deafened with kanamycine 14 days treatment (group I), single kanamycin injection followed by etacrinic acid administration (group II) and control group. Hearing evaluation performed with ABR recordings on 6th day after drug exposure
  
  
  RESULTS
  Both protocols were not able to guarantee complete ablation of the inner ear in tested animals. Although short deafening strategy was more effective (83.33% deaf mice) it was combined with high rate of mortality during general anesthesia for hearing evaluation.
  
  
  CONCLUSIONS
  Variable outcomes in deafening mouse animal model implies the necessity of hearing evaluation every time prior to the pathophysiological as well as molecular studies. Mice exposed to severe oto- and nephrotoxic insult do not recover after anesthetic drug administration, thus harvesting inner ear tissues especially as the source of RNA should be performed immediately after ABR recordings.
authors.affiliation: Klinika Otolaryngologii i Onkologii Laryngologicznej Uniwersytetu Medycznego w Poznaniu. wrobmac@ump.edu.pl
authors.forename: Maciej
authors.initials: M
authors.lastname: Wróbel
authors.forename: Michał
authors.initials: M
authors.lastname: Karlik
authors.forename: Marcin
authors.initials: M
authors.lastname: Szaumkssel
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Rydzanicz
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Szyfter
authors.forename: Witold
authors.initials: W
authors.lastname: Szyfter
mesh_terms: D000161:Acoustic Stimulation; D000818:Animals; D001309:Auditory Threshold; D003051:Cochlea / Q000473:pathology; D003638:Deafness / Q000139:chemically induced* / Q000473:pathology; D004195:Disease Models, Animal; D007758:Ear, Inner / Q000473:pathology; D004976:Ethacrynic Acid; D007612:Kanamycin; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D007612:Kanamycin; D004976:Ethacrynic Acid