title: [Formation and quantification assay of Candida albicans and Staphylococcus aureus mixed biofilm].
abstract: This study quantifies the production of single and mixed biofilms of Candida albicans and Staphylococcus aureus to determine if such mixed biofilms have synergistic effects. Assays were performed using polystyrene microtitre plates of 96 wells, metabolic activity was measured by the enzymatic reduction of a tetrazolium salt (XTT) and colorimetric changes were measured at 490 nm. Confocal scanning laser microscopy was used to visualise the biofilms of each microorganism and its growth kinetics. The highest levels of biofilm formation were observed in mixed biofilms, followed by those of Candida albicans only, with the lowest levels of biofilm formation being detected for Staphylococcus aureus; all together these results suggest a synergistic relationship between the tested microorganisms.
authors.affiliation: Instituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad Juárez, Ciudad Juárez, Chihuahua, México.
authors.forename: Consuelo
authors.initials: C
authors.lastname: Ibarra-Trujillo
authors.forename: María
authors.initials: M
authors.lastname: Villar-Vidal
authors.forename: Luis Alberto
authors.initials: LA
authors.lastname: Gaitán-Cepeda
authors.forename: Amaury
authors.initials: A
authors.lastname: Pozos-Guillen
authors.forename: Roberto
authors.initials: R
authors.lastname: Mendoza-de Elias
authors.forename: Luis Octavio
authors.initials: LO
authors.lastname: Sánchez-Vargas
mesh_terms: D018441:Biofilms; D002176:Candida albicans / Q000302:isolation & purification* / Q000502:physiology*; D006801:Humans; D013211:Staphylococcus aureus / Q000302:isolation & purification* / Q000502:physiology*