title: Preparation of chemotactically competent Dictyostelium cells.
abstract: Environmental changes result in signaling events in cell membranes. To develop the means to understand these events at the molecular level, it is essential to become familiar with the stochastic nature of signaling molecules in living cells. Using total internal reflection fluorescent microscopy (TIRFM), these signaling events can be directly observed at the single-molecule level. This protocol describes the preparation of chemotactically competent Dictyostelium cells. These cells are highly sensitive to chemoattractant stimulation and can be used to visualize single molecules with TIRFM.
authors.forename: Satomi
authors.initials: S
authors.lastname: Matsuoka
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Miyanaga
authors.forename: Toshio
authors.initials: T
authors.lastname: Yanagida
authors.forename: Masahiro
authors.initials: M
authors.lastname: Ueda
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D002633:Chemotaxis; D003584:Cytological Techniques / Q000379:methods*; D004023:Dictyostelium / Q000166:cytology*; D015398:Signal Transduction