title: The cartilage matrix molecule components produced by human foetal cartilage rudiment cells within scaffolds and the role of exogenous growth factors.
abstract: Development of cartilage lesions in osteoarthritis and following traumatic injury has important consequences on the weight bearing and articulation of joints, has severe impact on the quality of life of affected individuals and is of significant socioeconomic impact. Hyaline cartilage is a highly specialised tissue with a limited ability to self repair. Development of three-dimensional scaffolds which maintain the correct chondrocyte phenotype during expansion of cells in vitro and their application in regenerative strategies for cartilage repair is therefore a major research objective of many laboratories. This study examined the matrix components elaborated by cultured foetal cartilage rudiment cells, a mixture of chondroblasts/chondroprogenitor cells and committed chondrocytes, in monolayer, cell pellet cultures and in the synthetic scaffolds sodium alginate and polyglycolic acid (PGA). The ability of fibroblast growth factor (FGF)-2 and FGF-18 to promote chondrogenesis in pellet cultures was also examined. While the scaffolds did not completely replicate the matrix organisation evident in native cartilage, type II collagen and aggrecan were nevertheless prominent matrix components. FGF-2 and FGF-18 further promoted the production of cartilage-specific matrix components in pellet culture as FGF-18 stimulated the production of type X collagen and perlecan and may be indicative of a more terminally differentiated phenotype induced in the rudiment cells with this growth factor.
authors.affiliation: Graduate School of Biomedical Engineering, The University of New South Wales, Sydney NSW 2052, Australia.
authors.forename: Christine Y
authors.initials: CY
authors.lastname: Chuang
authors.forename: Kifah
authors.initials: K
authors.lastname: Shahin
authors.forename: Megan S
authors.initials: MS
authors.lastname: Lord
authors.forename: James
authors.initials: J
authors.lastname: Melrose
authors.forename: Pauline M
authors.initials: PM
authors.lastname: Doran
authors.forename: John M
authors.initials: JM
authors.lastname: Whitelock
mesh_terms: D053674:Aggrecans / Q000378:metabolism; D000464:Alginates / Q000737:chemistry; D002356:Cartilage / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D002478:Cells, Cultured; D020219:Chondrogenesis / Q000187:drug effects; D024043:Collagen Type II / Q000378:metabolism; D003470:Culture Media; D005109:Extracellular Matrix / Q000378:metabolism*; D005333:Fetus / Q000166:cytology / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D007150:Immunohistochemistry; D011100:Polyglycolic Acid / Q000737:chemistry; D013234:Stem Cells / Q000378:metabolism
chemical_list: D053674:Aggrecans; D000464:Alginates; D024043:Collagen Type II; D003470:Culture Media; D006603:Hexuronic Acids; C113569:fibroblast growth factor 18; D016222:Fibroblast Growth Factor 2; D011100:Polyglycolic Acid; D005346:Fibroblast Growth Factors; D020723:Glucuronic Acid