title: Protective role of N-trans-feruloyltyramine against β-amyloid peptide-induced neurotoxicity in rat cultured cortical neurons.
abstract: Enhanced oxidative stress and inflammation play important roles in the pathogenesis of Alzheimer's disease (AD). Amyloid β-peptide (Aβ), a major component of amyloid plaques, is considered to have a causal role in the development and progress of AD by being the initiator of a pathological cascade leading to oxidative stress. The present study investigated the effect of N-trans-feruloyltyramine (NTF) purified from Polyalthia suberosa, an alkaloid shown to protect against oxidative stress and cell death. Pre-treatment of rat primary cortical cell cultures with 25-250μM NTF significantly attenuated 10μM Aβ(1-42)-induced neuronal death in a dose-dependent manner. Apoptotic cell death was demonstrated morphologically as well as by detection of the presence of activated caspase-3 and Bax, levels of which could be reduced by NTF pre-treatment. NTF also reduced production of reactive oxygen species induced by Aβ(1-42). These findings suggest that the protective effect of NTF against Aβ(1-42)-induced neuronal death might be due to its antioxidative property.
authors.affiliation: Research Center for Neuroscience, Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhonpathom, Thailand. grwtn@mahidol.ac.th
authors.forename: Wipawan
authors.initials: W
authors.lastname: Thangnipon
authors.forename: Nirut
authors.initials: N
authors.lastname: Suwanna
authors.forename: Narisorn
authors.initials: N
authors.lastname: Kitiyanant
authors.forename: Rungtip
authors.initials: R
authors.lastname: Soi-Ampornkul
authors.forename: Patoomratana
authors.initials: P
authors.lastname: Tuchinda
authors.forename: Bamroong
authors.initials: B
authors.lastname: Munyoo
authors.forename: Saksit
authors.initials: S
authors.lastname: Nobsathian
mesh_terms: D016229:Amyloid beta-Peptides / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D003373:Coumaric Acids / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D014439:Tyramine / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D016229:Amyloid beta-Peptides; D003373:Coumaric Acids; D018696:Neuroprotective Agents; D017382:Reactive Oxygen Species; C074004:feruloyltyramine; D053148:Caspase 3; D014439:Tyramine