title: [Assessment of in vitro activity and stability of antifungal suspensions for mouthrinses: to a reappraisal of empiric practices?].
abstract: Establishment of an effective prophylaxis against oral candidiasis by local treatment is essential for immunocompromised patients. The aim of the study is to assess effectiveness and stability of antifungal suspensions for mouthrinses. The assessed suspensions are compounded by one solvent among sterile water, spring water or sodium bicarbonate associated with amphotericin B (Fungizone®) or nystatine (Mycostatine®). Two others mixes are assessed: Mycostatine®-bicarbonate and Mycostatine®-Hextril®-bicarbonate as well as the two straight antifungal. In vitro activity is tested on five Candida species (C. albicans, C. glabrata, C. krusei, C. parapsilosis, C. tropicalis) after a five minutes contact between yeasts and the assessed suspension. A galenic study is realized during 3 days. Mixes associating a polyene with sodium bicarbonate have no effectiveness on Candida albicans, others mixes shows intermediate effectiveness (the percentage of yeast growth inhibition lies between 35% and 68%). Effectiveness results of Hextril®-based mixes are not explainable because of alcohol in its composition. Spring water-based mixes must be evicted due to microbiologic contaminations after 48hours. Mycostatine®-Hextril®-bicarbonate mix is not stable during 3 days. All those mouthrinses, poorly effective, excepted on C. glabrata, should be avoided. Straight Mycostatine® shows a good antifungal effectiveness excepted on C. krusei and its use should be recommended.
authors.affiliation: Pharmacie Lapeyronie-Arnaud-de-Villeneuve, centre hospitalier régional universitaire de Montpellier, 191 avenue du Doyen-Gaston-Giraud, Montpellier, France. jolivotpa@aol.fr
authors.forename: P A
authors.initials: PA
authors.lastname: Jolivot
authors.forename: C
authors.initials: C
authors.lastname: Dunyach-Remy
authors.forename: A
authors.initials: A
authors.lastname: Roussey
authors.forename: A
authors.initials: A
authors.lastname: Jalabert
authors.forename: M
authors.initials: M
authors.lastname: Mallié
authors.forename: S
authors.initials: S
authors.lastname: Hansel-Esteller
mesh_terms: D000666:Amphotericin B / Q000008:administration & dosage; D000935:Antifungal Agents / Q000008:administration & dosage*; D002175:Candida / Q000187:drug effects; D002176:Candida albicans / Q000187:drug effects; D041221:Candida glabrata / Q000187:drug effects; D002180:Candidiasis, Oral / Q000188:drug therapy*; D004355:Drug Stability; D006590:Hexetidine; D006801:Humans; D009067:Mouthwashes; D009761:Nystatin / Q000008:administration & dosage; D017693:Sodium Bicarbonate / Q000008:administration & dosage; D013535:Suspensions
chemical_list: D000935:Antifungal Agents; D009067:Mouthwashes; D013535:Suspensions; D009761:Nystatin; D000666:Amphotericin B; D006590:Hexetidine; D017693:Sodium Bicarbonate