title: Development of a robust cytopathic effect-based high-throughput screening assay to identify novel inhibitors of dengue virus.
abstract: We have developed a robust cytopathic effect-based high-throughput screening assay to identify inhibitors of dengue virus (DENV) infection. Screening of a small natural product library yielded 11 hits. Four of these were found to be potent inhibitors of DENV, although serotype differences were noted. Taken together, these data suggest that screening of larger and more complex molecule libraries may result in the identification of more potent and specific DENV inhibitors.
authors.affiliation: Department of Infectious Diseases and Microbiology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
authors.forename: Kevin D
authors.initials: KD
authors.lastname: McCormick
authors.forename: Shufeng
authors.initials: S
authors.lastname: Liu
authors.forename: Jana L
authors.initials: JL
authors.lastname: Jacobs
authors.forename: Ernesto T A
authors.initials: ET
authors.lastname: Marques
authors.forename: Nicolas
authors.initials: N
authors.lastname: Sluis-Cremer
authors.forename: Tianyi
authors.initials: T
authors.lastname: Wang
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D003716:Dengue Virus / Q000187:drug effects*; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans
chemical_list: D000998:Antiviral Agents