title: Alphab-crystallin expression in epiretinal membrane of human proliferative diabetic retinopathy.
abstract: OBJECTIVE
  To examine the expression of alphaB-crystallin and its colocalization with vascular endothelial growth factor in the epiretinal membrane of human proliferative diabetic retinopathy.
  
  
  METHODS
  Ten epiretinal membranes of proliferative diabetic retinopathy and three normal retinas surgically excised were included in this study. Paraformaldehyde-fixed, paraffin-embedded tissue sections were processed for immunohistochemistry with alphaB-crystallin, vascular endothelial growth factor, and CD31 antibodies.
  
  
  RESULTS
  AlphaB-crystallin was expressed in all epiretinal membranes examined. The immunolocalization of alphaB-crystallin was detected in the cytoplasm of CD31-positive endothelial cells, but not in normal retinal blood vessels. Furthermore, alphaB-crystallin immunoreactivity was colocalized in vascular endothelial growth factor-positive endothelial cells in proliferative diabetic retinopathy membranes.
  
  
  CONCLUSIONS
  AlphaB-crystallin was expressed in proliferative diabetic retinopathy membranes, and colocalized with vascular endothelial growth factor-positive neovessels. AlphaB-crystallin may play a potential role in the pathogenesis of epiretinal membranes in proliferative diabetic retinopathy, together with vascular endothelial growth factor.
authors.affiliation: Laboratory of Ocular Cell Biology and Visual Science, Department of Ophthalmology, Hokkaido University Graduate School of Medicine, Sapporo, Hokkaido, Japan.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Dong
authors.forename: Satoru
authors.initials: S
authors.lastname: Kase
authors.forename: Ryo
authors.initials: R
authors.lastname: Ando
authors.forename: Junichi
authors.initials: J
authors.lastname: Fukuhara
authors.forename: Wataru
authors.initials: W
authors.lastname: Saito
authors.forename: Atsuhiro
authors.initials: A
authors.lastname: Kanda
authors.forename: Miyuki
authors.initials: M
authors.lastname: Murata
authors.forename: Kousuke
authors.initials: K
authors.lastname: Noda
authors.forename: Susumu
authors.initials: S
authors.lastname: Ishida
mesh_terms: D000328:Adult; D000368:Aged; D003593:Cytoplasm / Q000378:metabolism; D003930:Diabetic Retinopathy / Q000378:metabolism*; D042783:Endothelial Cells / Q000378:metabolism; D019773:Epiretinal Membrane / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D012160:Retina / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D038203:alpha-Crystallin B Chain / Q000378:metabolism*
chemical_list: D042461:Vascular Endothelial Growth Factor A; D038203:alpha-Crystallin B Chain