title: Prevalence of Ancylostoma braziliense in cats in three northern counties of Florida, United States.
abstract: A convenience sampling of fecal specimens from 40 cats in northern Florida was examined for the presence of Ancylostoma braziliense eggs by using centrifugal sugar flotation and polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP). Of the 40 samples, 26 (65%) contained hookworm eggs. DNA from 24 samples was successfully amplified using PCR; using RFLP, 10 samples were identified as containing DNA of A. braziliense (41.7% of the 24 samples that successfully amplified). Of these, 6 samples contained DNA of both Ancylostoma tubaeforme and A. braziliense, and 4 samples contained only DNA of A. braziliense. The remaining samples (n  =  14) contained only the DNA of A. tubaeforme, except for 1 sample that had no discernible bands after RFLP.
authors.affiliation: Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853-6401, USA.
authors.forename: Janice L
authors.initials: JL
authors.lastname: Liotta
authors.forename: Khuanchai N
authors.initials: KN
authors.lastname: Koompapong
authors.forename: Joseph P
authors.initials: JP
authors.lastname: Yaros
authors.forename: Joseph
authors.initials: J
authors.lastname: Prullage
authors.forename: Dwight D
authors.initials: DD
authors.lastname: Bowman
mesh_terms: D000722:Ancylostoma / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D000724:Ancylostomiasis / Q000453:epidemiology / Q000662:veterinary*; D000818:Animals; D002371:Cat Diseases / Q000453:epidemiology* / Q000469:parasitology; D002415:Cats; D005243:Feces / Q000469:parasitology; D005431:Florida / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D015995:Prevalence