title: Pseudorhodoferax aquiterrae sp. nov., isolated from groundwater.
abstract: A Gram-negative, rod-shaped, motile, non-spore-forming and poly-β-hydroxybutyrate-containing bacterial strain, designated NAFc-7(T), was isolated from groundwater in Taiwan and was characterized using a polyphasic taxonomic approach. Growth occurred at 15-40 °C (optimum, 30 °C), at pH 6.0-9.0 (optimum, pH 6.0-7.0) and with 0-3 % NaCl (optimum, 0-1 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain NAFc-7(T), together with Pseudorhodoferax soli TBEA3(T) (98.2 % sequence similarity) and Pseudorhodoferax caeni SB1(T) (98.0 %), formed a deep line within the family Comamonadaceae. Strain NAFc-7(T) contained summed feature 3 (comprising C(16 : 1)ω7c and/or C(16 : 1)ω6c), C(16 : 0) and C(17 : 0) cyclo as the predominant fatty acids. The major 3-hydroxy fatty acid was C(10 : 0) 3-OH. The major isoprenoid quinone was Q-8 and the DNA G+C content was 67.6 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylserine and an uncharacterized phospholipid. The DNA-DNA relatedness of strain NAFc-7(T) with respect to recognized species of the genus Pseudorhodoferax was less than 70 %. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain NAFc-7(T) represents a novel member of the genus Pseudorhodoferax, for which the name Pseudorhodoferax aquiterrae sp. nov. is proposed. The type strain is NAFc-7(T) (= BCRC 80210(T) = LMG 26333(T) = KCTC 23314(T)).
authors.affiliation: Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, 142 Hai-Chuan Road Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
authors.forename: Wen-Ming
authors.initials: WM
authors.lastname: Chen
authors.affiliation: Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, 142 Hai-Chuan Road Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
authors.forename: Yang-Shun
authors.initials: YS
authors.lastname: Lin
authors.affiliation: Department of Soil Environmental Science, College of Agriculture and Natural Resources, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 402, Taiwan, ROC.
authors.forename: Chiu-Chung
authors.initials: CC
authors.lastname: Young
authors.affiliation: Department of Marine Biotechnology, National Kaohsiung Marine University, 142 Hai-Chuan Road Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
authors.forename: Shih-Yi
authors.initials: SY
authors.lastname: Sheu
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D042621:Comamonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D060587:Groundwater / Q000382:microbiology*; D006885:Hydroxybutyrates / Q000032:analysis; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011091:Polyesters / Q000032:analysis; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013624:Taiwan; D014871:Water Microbiology; D000094122:Polyhydroxybutyrates
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D006885:Hydroxybutyrates; D010743:Phospholipids; D011091:Polyesters; D011809:Quinones; D012336:RNA, Ribosomal, 16S; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates