title: Fabrication of glyconanoparticle microarrays.
abstract: We report a new type of microarray, based on glyconanoparticles (GNPs), to study glycan-lectin interactions. GNPs, synthesized by conjugating carbohydrate ligands on silica nanoparticles, were printed on a photoactive surface followed by covalent immobilization by light activation. The GNP microarrays could be probed by lectins labeled with fluorescein as well as fluorescein-doped silica nanoparticles (FSNPs). Results showed that FSNP as the label enhanced the signals for the higher affinity ligands than the lower ones.
authors.forename: Qi
authors.initials: Q
authors.lastname: Tong
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Takuya
authors.initials: T
authors.lastname: Kubo
authors.forename: Mingdi
authors.initials: M
authors.lastname: Yan
mesh_terms: D037102:Lectins / Q000378:metabolism; D008027:Light; D046228:Microarray Analysis / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism*; D011327:Printing; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D037102:Lectins; D011134:Polysaccharides; D012822:Silicon Dioxide