title: The effects of NB-UVB on the hair follicle-derived neural crest stem cells differentiating into melanocyte lineage in vitro.
abstract: BACKGROUND
  Narrow-band UVB (NB-UVB) is an effective therapeutic option in the treatment of vitiligo. Despite the apparent clinical efficacy, the underlying mechanism of how topical NB-UVB induces repigmentation in vitiligo has not been clearly elucidated.
  
  
  OBJECTIVE
  To investigate the effects of NB-UVB on the maturation of melanocyte lineage differentiated from hair follicle-derived neural crest stem cells (HF-NCSCs) in vitro.
  
  
  METHODS
  HF-NCSCs were isolated from mouse whisker follicles. The isolated cells were multipotent and expressed embryonic NCSC biomarkers. The effects of NB-UVB on development and differentiation of HF-NCSCs were evaluated. We assessed cell viability, melanogenesis and migration of melanocytes derived from HF-NCSCs after NB-UVB radiation. Tyrosinase, Tyrp1, Dct, Kit, Mc1R, Fzd4, NT3R, Ednra, EP1, TGFβR, Sox10, Mitf, Lef1 and Pax3 gene expression was measured by quantitative RT-PCR, while Tyrosinase, Sox10 and Mitf protein expression were measured by Western blot analysis. Cell migration was measured by Boyden chamber transwell assay.
  
  
  RESULTS
  NB-UVB increased the expression of tyrosinase during melanocytic differentiation from mouse HF-NCSCs, however, NB-UVB inhibited proliferation of melanocytes derived from HF-NCSCs. Mechanistically, increased melanocyte maturation after NB-UVB treatment was resulted from increased expression of several key melanogenic factors, including Sox10, Kit and Mc1R, which play a critical role to promote tyrosinase expression. Furthermore, the migration of the HF-NCSCs-derived melanocytes was downregulated as NB-UVB doses increased. However, the migration of HF-NCSCs was upregulated under 0.4J NB-UVB radiation.
  
  
  CONCLUSIONS
  Those data provide in vitro evidence demonstrating some direct effects of NB-UVB on pigmentation of melanocyte lineage differentiated from HF-NCSCs, and may provide a possible mechanism for the effect of NB-UVB in vitiligo.
authors.affiliation: Department of Dermatology, Huashan Hospital, Fudan University, Shanghai, PR China.
authors.forename: Dake
authors.initials: D
authors.lastname: Dong
authors.forename: Min
authors.initials: M
authors.lastname: Jiang
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Xu
authors.forename: Ming
authors.initials: M
authors.lastname: Guan
authors.forename: Jiaqiang
authors.initials: J
authors.lastname: Wu
authors.forename: Qinyi
authors.initials: Q
authors.lastname: Chen
authors.forename: Leihong
authors.initials: L
authors.lastname: Xiang
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000502:physiology / Q000528:radiation effects; D002454:Cell Differentiation / Q000502:physiology / Q000528:radiation effects; D019070:Cell Lineage / Q000502:physiology / Q000528:radiation effects; D002465:Cell Movement / Q000502:physiology / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D018859:Hair Follicle / Q000166:cytology* / Q000528:radiation effects*; D066298:In Vitro Techniques; D019746:Intramolecular Oxidoreductases / Q000235:genetics / Q000378:metabolism; D008544:Melanocytes / Q000166:cytology*; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D014442:Monophenol Monooxygenase / Q000235:genetics / Q000378:metabolism; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000528:radiation effects*; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics / Q000378:metabolism; D055757:SOXE Transcription Factors / Q000235:genetics / Q000378:metabolism; D015533:Transcriptional Activation / Q000502:physiology / Q000528:radiation effects; D014467:Ultraviolet Therapy / Q000379:methods*
chemical_list: D008562:Membrane Glycoproteins; D055757:SOXE Transcription Factors; C527924:Sox10 protein, mouse; D010088:Oxidoreductases; C079621:Tyrp1 protein, mouse; D014442:Monophenol Monooxygenase; D019009:Proto-Oncogene Proteins c-kit; D019746:Intramolecular Oxidoreductases; C042501:dopachrome isomerase