title: [Study on fibrolytic and hypotensive activity of earthworm homogenate of ultrafiltration separation from enzymolysis].
abstract: OBJECTIVE
  To study the fibrolytic and hypotensive activity of earthworm homogenate of ultrafiltration separation from simulated enzymolysis of gastrointestinal tract system.
  
  
  METHODS
  The before and after enzymolysis homogenate of fresh earthworm was seperated with different pore size PVDF ultrafiltration membrane and its fibrolytic and hypotensive activity was assayed.
  
  
  RESULTS
  The fibrolytic activity of the total homogenate after enzymolysis overall changed little, but decreased in the the site of higher molecular weight and increased in the lower site of molecular weight; The ACE inhibitory activity improved, especially in the filtrate of the MW 4000 membrane.
  
  
  CONCLUSIONS
  The fibrolytic activity of earthworm homogenate was not reduced by the digestive simulated enzymolysis, and the retention site of MW 10 000 membrane have more fibrolytic activity; The hypotensive activity of earthworm homogenate is enhanced by the digestive simulated enzymolysis. So the stronger activity could be obtained from enzymolysis.
authors.affiliation: The Pre-clinical Medicine College, Nanjing University of TCM, Nanjing 210029, China. xqzhan14@sina.com
authors.forename: Xiu-Qin
authors.initials: XQ
authors.lastname: Zhan
authors.forename: Qi-Gang
authors.initials: QG
authors.lastname: Dai
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D000959:Antihypertensive Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005342:Fibrinolysis; D005343:Fibrinolytic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D006867:Hydrolases / Q000378:metabolism; D006868:Hydrolysis; D008419:Materia Medica / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008567:Membranes, Artificial; D008970:Molecular Weight; D009835:Oligochaeta; D014462:Ultrafiltration / Q000379:methods
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000959:Antihypertensive Agents; D005343:Fibrinolytic Agents; D008419:Materia Medica; D008567:Membranes, Artificial; D006867:Hydrolases