title: In vitro effect of clinical propofol concentrations on red blood cell aggregation and deformability.
abstract: This in vitro study investigated time-related effects of propofol at the plasma concentrations required for sedation and general anaesthesia, on RBC aggregation, deformability, and morphology. Blood containing propofol at plasma concentrations of 0, 2 and 4 μg ml-1 was incubated in a water bath at 37°C for 1, 2, or 4 hours. RBC elongation indices (EIs) and aggregation indices (AIs), which represent RBC deformability and aggregation, respectively, were measured. Also, RBC morphological indices (MIs), which represent RBC morphology, were calculated. EIs and AIs were similar at propofol concentrations of 0, 2, or 4 μg ml-1 after 1, 2, or 4 hours of incubation. MIs at propofol plasma concentrations 0 or 2 μg ml-1 were similar after 1, 2, and 4 hours of incubation, however, MI at a propofol concentration of 4 μg ml-1 after 4 hours of incubation was higher than its value after 1 or 2 hours of incubation. No significant difference was observed between MIs at propofol plasma concentrations 0, 2, or 4 μg ml-1 after 1, 2, and 4 hours of incubation. At clinical doses, propofol has no direct effects on RBC deformability, aggregation, or morphology over a 4 hours incubation period.
authors.affiliation: Department of Anaesthesiology and Pain Medicine, College of Medicine, Korea University, Seoul, Republic of Korea.
authors.forename: Yun Hee
authors.initials: YH
authors.lastname: Kim
authors.forename: Hye Gyo
authors.initials: HG
authors.lastname: Chung
authors.forename: Soohyun A
authors.initials: SA
authors.lastname: Myung
authors.forename: Jee Hyun
authors.initials: JH
authors.lastname: Rha
authors.forename: Sung
authors.initials: S
authors.lastname: Yang
authors.forename: Myung Hyun
authors.initials: MH
authors.lastname: Nam
authors.forename: Se Hyun
authors.initials: SH
authors.lastname: Shin
authors.forename: Choon Hak
authors.initials: CH
authors.lastname: Lim
mesh_terms: D000328:Adult; D004903:Erythrocyte Aggregation / Q000187:drug effects*; D004907:Erythrocyte Deformability / Q000187:drug effects*; D004912:Erythrocytes / Q000187:drug effects / Q000473:pathology; D006801:Humans; D066298:In Vitro Techniques; D015742:Propofol / Q000494:pharmacology*
chemical_list: D015742:Propofol