title: Endothelialization and in-stent restenosis on the surface of glycoprotein IIIa monoclonal antibody eluting stent.
abstract: Since the percutaneous transtuminal coronary angioplasty was introduced into China in 1984, this procedure has become widely accepted as an important step in coronary revascularization. This study shows the effect of the monoclonal antibody (mAb) on the platelet glycoprotein IIIa receptor during endothelialization and in-stent restenosis by implanting the mAb-eluting stents into iliac arteries of rabbits. The hard tissue cross sections of the stent-implanted arterial segments were made by polymethylmethacrylate embedding. Arterial intima proliferation was observed and analyzed. The endothelialization of the stent surface was observed using scanning electron microscope, whereas the ultrastructure of the neointima was observed using transmission electron microscope. After one month of stent implantation, the surfaces of both groups were covered by intact endothelial layers, but the neointimal areas and the ratio of stenosis were significantly lesser in the mAb-eluting stent group (p < 0.01). After 3 months, the ratio of stenosis in the mAb-eluting stent group was 14.67 ± 0.79, whereas that of the bare stent group was 21.58 ± 1.76 (p < 0.01). Therefore, the mAb eluting from the stent surface has the potential to accelerate endothelialization, prevent thrombosis formation due to the interaction of stent with blood, and decrease the stenosis ratio by inhibiting neointima proliferation.
authors.affiliation: Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College of Chongqing University, Chongqing University, Chongqing 400030, China.
authors.forename: Tie-Ying
authors.initials: TY
authors.lastname: Yin
authors.forename: Gui-Xue
authors.initials: GX
authors.lastname: Wang
authors.forename: De-Chuan
authors.initials: DC
authors.lastname: Zhang
authors.forename: Ding-Yuan
authors.initials: DY
authors.lastname: Du
authors.forename: Zheng-Gong
authors.initials: ZG
authors.lastname: Li
authors.forename: Lai-Long
authors.initials: LL
authors.lastname: Luo
authors.forename: Yan-Bin
authors.initials: YB
authors.lastname: Hou
authors.forename: Ya-Zhou
authors.initials: YZ
authors.lastname: Wang
authors.forename: Jing-Bo
authors.initials: JB
authors.lastname: Zhao
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage* / Q000276:immunology; D001158:Arteries / Q000276:immunology / Q000648:ultrastructure; D023903:Coronary Restenosis / Q000517:prevention & control*; D054855:Drug-Eluting Stents; D004730:Endothelium, Vascular / Q000276:immunology / Q000648:ultrastructure; D039661:Integrin beta3 / Q000276:immunology*; D008297:Male; D058426:Neointima / Q000517:prevention & control; D011817:Rabbits; D013927:Thrombosis / Q000517:prevention & control
chemical_list: D000911:Antibodies, Monoclonal; D039661:Integrin beta3