title: Safe high-pressure freezing of infectious micro-organisms.
abstract: We describe how high-pressure freezing of infectious biological material can safely be accomplished with the help of membrane carriers. The method described is easy to perform; however, careful manipulations are required. Existing safety regulations must still be followed. However, the procedure reduces the risk of dissemination of infectious material.
authors.affiliation: Institute of Anatomy, Experimental Morphology, University of Bern, Bern, Switzerland.
authors.forename: D
authors.initials: D
authors.lastname: Vanhecke
authors.forename: B
authors.initials: B
authors.lastname: Zuber
authors.forename: S D
authors.initials: SD
authors.lastname: Brugger
authors.forename: D
authors.initials: D
authors.lastname: Studer
mesh_terms: D003264:Containment of Biohazards; D015925:Cryopreservation / Q000295:instrumentation / Q000379:methods*; D004869:Equipment Safety; D006801:Humans; D006874:Hydrostatic Pressure; D008854:Microscopy, Electron; D013296:Streptococcus pneumoniae / Q000502:physiology*