title: Plasmid pP62BP1 isolated from an Arctic Psychrobacter sp. strain carries two highly homologous type II restriction-modification systems and a putative organic sulfate metabolism operon.
abstract: The complete nucleotide sequence of plasmid pP62BP1 (34,467 bp), isolated from Arctic Psychrobacter sp. DAB_AL62B, was determined and annotated. The conserved plasmid backbone is composed of several genetic modules, including a replication system (REP) with similarities to the REP region of the iteron-containing plasmid pPS10 of Pseudomonas syringae. The additional genetic load of pP62BP1 includes two highly related type II restriction-modification systems and a set of genes (slfRCHSL) encoding enzymes engaged in the metabolism of organic sulfates, plus a putative transcriptional regulator (SlfR) of the AraC family. The pP62BP1 slf                         locus has a compact and unique structure. It is predicted that the enzymes SlfC, SlfH, SlfS and SlfL carry out a chain of reactions leading to the transformation of alkyl sulfates into acyl-CoA, with dodecyl sulfate (SDS) as a possible starting substrate. Comparative analysis of the nucleotide sequences of pP62BP1 and other Psychrobacter spp. plasmids revealed their structural diversity. However, the presence of a few highly conserved DNA segments in pP62BP1, plasmid 1 of P. cryohalolentis K5 and pRWF-101 of Psychrobacter sp. PRwf-1 is indicative of recombinational shuffling of genetic information, and is evidence of lateral gene transfer in the Arctic environment.
authors.affiliation: Department of Bacterial Genetics, Faculty of Biology, Institute of Microbiology, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland.
authors.forename: Robert
authors.initials: R
authors.lastname: Lasek
authors.forename: Lukasz
authors.initials: L
authors.lastname: Dziewit
authors.forename: Dariusz
authors.initials: D
authors.lastname: Bartosik
mesh_terms: D015280:DNA Restriction-Modification Enzymes / Q000235:genetics* / Q000378:metabolism; D022761:Gene Transfer, Horizontal / Q000502:physiology*; D005798:Genes, Bacterial / Q000502:physiology*; D010957:Plasmids; D044096:Psychrobacter / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA / Q000379:methods; D013463:Sulfuric Acid Esters / Q000378:metabolism*
chemical_list: D015280:DNA Restriction-Modification Enzymes; D013463:Sulfuric Acid Esters