title: A protein biosensor that relies on bending of single DNA molecules.
abstract: A "bendy" protein sensor: A DNA-based sensor that uses folded DNA (through DNA kinks) and protein-induced bending to detect DNA-binding proteins is presented. Single-molecule sensing of a transcriptional activator (catabolite activator protein, CAP, which bends its DNA site by 80°) is demonstrated in solution and on surfaces, both in buffers and in cell lysates. The method should allow detection of a wide range of DNA-bending proteins.
authors.affiliation: Biological Physics Research Group, Clarendon Laboratory, Department of Physics, University of Oxford, UK.
authors.forename: Robert
authors.initials: R
authors.lastname: Crawford
authors.forename: Douglas J
authors.initials: DJ
authors.lastname: Kelly
authors.forename: Achillefs N
authors.initials: AN
authors.lastname: Kapanidis
mesh_terms: D001665:Binding Sites; D015374:Biosensing Techniques; D002373:Cyclic AMP Receptor Protein / Q000737:chemistry* / Q000378:metabolism; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer; D009690:Nucleic Acid Conformation
chemical_list: D002373:Cyclic AMP Receptor Protein; D004247:DNA