title: Anticonvulsant and antioxidant effects of cyano-carvone and its action on acetylcholinesterase activity in mice hippocampus.
abstract: The anticonvulsant effect of cyano-carvone, a monoterpene monocyclic, was investigated in epilepsy model induced by pilocarpine. Cyano-carvone at doses of 25, 50 or 75 mg/kg promoted a reduction of 16.7, 33 and 66.7%, respectively, against pilocarpine-induced seizures, and it was efficacious in increasing both the latency to first seizures and the survival percentage, resulting in 33.3, 67 and 91.7% of protection against death induced by seizures, respectively (P < 0.05). The reference drug atropine (25 mg/kg) also produced a significant protection (100%). Its monoterpene, at 25, 50 and 75 mg/kg, was also capable to increase the latency for installation of status epilepticus induced by pilocarpine, and presented a significant protection against lipid peroxidation and nitrite formation in mice hippocampus (P < 0.05). In addition, it was observed that the cyano-carvone pretreatment increased the acetylcholinesterase activity in mice hippocampus after pilocarpine-induced seizures. The present results clearly indicate the anticonvulsant ability of cyano-carvone, which can be, at least in part, explained by the increased activity of the acetylcholinesterase enzyme. Our data suggest that the action mechanism can also be due to a direct activation of the antioxidant enzymes that could be associated with a reduction observed in oxidative stress in mice hippocampus, probably involving an inhibition of free radical production.
authors.affiliation: Laboratory of Research in Experimental Neurochemistry of Post-Graduation Program in Pharmaceutics Science, Federal University of Piauí, Teresina, Piauí, Brazil.
authors.forename: Dayane Alves
authors.initials: DA
authors.lastname: Costa
authors.forename: Guilherme Antônio Lopes
authors.initials: GA
authors.lastname: de Oliveira
authors.forename: Tamires Cardoso
authors.initials: TC
authors.lastname: Lima
authors.forename: Pauline Sousa
authors.initials: PS
authors.lastname: dos Santos
authors.forename: Damião Pergentino
authors.initials: DP
authors.lastname: de Sousa
authors.forename: Rivelilson Mendes
authors.initials: RM
authors.lastname: de Freitas
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology* / Q000627:therapeutic use; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use; D004195:Disease Models, Animal; D004789:Enzyme Activation / Q000187:drug effects; D008297:Male; D051379:Mice; D039821:Monoterpenes / Q000494:pharmacology* / Q000627:therapeutic use; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D013226:Status Epilepticus / Q000188:drug therapy* / Q000201:enzymology / Q000378:metabolism*
chemical_list: D000927:Anticonvulsants; D000975:Antioxidants; D039821:Monoterpenes; D000110:Acetylcholinesterase