title: Amyloid β-peptide neurotoxicity assay using cultured rat cortical neurons.
abstract: Neurons cultured from rodent central nervous system tissues represent an important tool in the study of neurodegenerative disease mechanisms and neuroregenerative processes, including the survival- and axon growth-promoting properties of neurotrophic factors. This chapter presents a detailed protocol for the preparation of rat and mouse cortical and hippocampal neuron cell cultures, using either embryonic or postnatal tissue.
authors.affiliation: Department of Pharmacology and Anesthesiology, University of Padova, Padova, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Facci
authors.forename: Stephen D
authors.initials: SD
authors.lastname: Skaper
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D016229:Amyloid beta-Peptides / Q000633:toxicity*; D000818:Animals; D002540:Cerebral Cortex / Q000166:cytology* / Q000196:embryology*; D057075:Enzyme Assays / Q000379:methods; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008163:Luminescent Measurements / Q000379:methods; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D051381:Rats
chemical_list: D016229:Amyloid beta-Peptides; D000255:Adenosine Triphosphate; D007770:L-Lactate Dehydrogenase