title: Measuring markers of liver function using a micropatterned paper device designed for blood from a fingerstick.
abstract: This paper describes a paper-based microfluidic device that measures two enzymatic markers of liver function (alkaline phosphatase, ALP, and aspartate aminotransferase, AST) and total serum protein. A device consists of four components: (i) a top plastic sheet, (ii) a filter membrane, (iii) a patterned paper chip containing the reagents necessary for analysis, and (iv) a bottom plastic sheet. The device performs both the sample preparation (separating blood plasma from erythrocytes) and the assays; it also enables both qualitative and quantitative analysis of data. The data obtained from the paper-microfluidic devices show standard deviations in calibration runs and "spiked" standards that are acceptable for routine clinical use. This device illustrates a type of test useable for a range of assays in resource-poor settings.
authors.affiliation: Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, United States.
authors.forename: Sarah J
authors.initials: SJ
authors.lastname: Vella
authors.forename: Patrick
authors.initials: P
authors.lastname: Beattie
authors.forename: Rebecca
authors.initials: R
authors.lastname: Cademartiri
authors.forename: Anna
authors.initials: A
authors.lastname: Laromaine
authors.forename: Andres W
authors.initials: AW
authors.lastname: Martinez
authors.forename: Scott T
authors.initials: ST
authors.lastname: Phillips
authors.forename: Katherine A
authors.initials: KA
authors.lastname: Mirica
authors.forename: George M
authors.initials: GM
authors.lastname: Whitesides
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D001219:Aspartate Aminotransferases / Q000378:metabolism; D001798:Blood Proteins / Q000032:analysis*; D002138:Calibration; D004867:Equipment Design; D005374:Filtration / Q000295:instrumentation; D006801:Humans; D008099:Liver / Q000201:enzymology*; D008111:Liver Function Tests / Q000295:instrumentation*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D010209:Paper; D012680:Sensitivity and Specificity
chemical_list: D001798:Blood Proteins; D001219:Aspartate Aminotransferases; D000469:Alkaline Phosphatase