title: [Effects of interferon-α combined with homoharringtonine on K562 cell proliferation and β-catenin expression].
abstract: The study was aimed to investigate the synergistically effect of interferon-α (IFN-α) and homoharringtonine (HHT) on the proliferation, apoptosis, cell cycle of K562 cells and the expression of β-catenin. The proliferation, apoptosis, cell cycle and β-catenin mRNA expression of K562 cells treated with IFN-α and/or HHT were assayed with MTT, flow cytometry or RT-PCR respectively. The results showed that HHT alone, but not IFN-α alone, displayed a proliferation inhibition, apoptosis induction, G(0)/G(1) phase block and down-regulation of β-catenin expression in K562 cells with concentration- and time-dependent manners. The expression level of β-catenin mRNA after being treated with HHT was 0.5576 ± 0.0373, which were lower than that in control group (0.9369 ± 0.0142). The down-regulation of β-catenin expression in group of IFN-α combined with HHT was higher significantly than that in HHT group (0.3737 ± 0.0529 vs 0.5576 ± 0.0373, P < 0.05). Otherwise, HHT combined with IFN-α did not demonstrate obvious toxicologic effect on bone marrow mononuclear cells. It is concluded that IFN-α combined with HHT can enhance the cytotoxic effect of HHT on K562 cells, which may be associated with down-regulation of β-catenin expression.
authors.affiliation: Department of Hematology, Nanjing Medical University, Huaian, Jiangsu Province, China.
authors.forename: Yu-Ye
authors.initials: YY
authors.lastname: Shi
authors.forename: Wei-Ke
authors.initials: WK
authors.lastname: Cao
authors.forename: Xiao-Ning
authors.initials: XN
authors.lastname: Liu
authors.forename: Zhi-Kui
authors.initials: ZK
authors.lastname: Deng
authors.forename: Hua
authors.initials: H
authors.lastname: Guo
authors.forename: Wan-Ting
authors.initials: WT
authors.lastname: Feng
authors.forename: Li-Lin
authors.initials: LL
authors.lastname: Ye
authors.forename: Jia-Bing
authors.initials: JB
authors.lastname: Zhu
authors.forename: Yu-Feng
authors.initials: YF
authors.lastname: Li
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects*; D006248:Harringtonines / Q000494:pharmacology*; D000077863:Homoharringtonine; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology*; D020014:K562 Cells; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism*
chemical_list: C495270:CTNNB1 protein, human; D006248:Harringtonines; D016898:Interferon-alpha; D051176:beta Catenin; D000077863:Homoharringtonine