title: Protein-imprinted materials: rational design, application and challenges.
abstract: Protein imprinting is a promising tool for generating artificial biomimetic receptors with antibody-like specific recognition sites. Recently, protein-imprinted materials, as potential antibody substitutes, have attracted much attention in many fields, for example chemical sensors, chromatographic stationary phases, and artificial enzymes, owing to their long-term storage stability, potential re-usability, resistance to harsh environment, and low cost. In this critical review, we focus our discussion on the rational preparation of protein-imprinted materials in terms of choice of template, functional monomer, crosslinker, and polymerization format. In addition, several highlighted applications of protein-imprinted materials are emphasized, not only in well-known fields but also in some unique fields, for example proteomics and tissue engineering. Finally, we propose challenges arising from the intrinsic properties of protein imprinting, for example obtaining the template, heterogeneous binding, and extrinsic competition, for example immobilized aptamers.
authors.affiliation: National Chromatographic R. & A. Center, Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian, China.
authors.forename: Kaiguang
authors.initials: K
authors.lastname: Yang
authors.forename: Lihua
authors.initials: L
authors.lastname: Zhang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Liang
authors.forename: Yukui
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D003432:Cross-Linking Reagents / Q000737:chemistry*; D006801:Humans; D008958:Models, Molecular; D054802:Molecular Imprinting / Q000379:methods*; D058105:Polymerization; D011108:Polymers / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D040901:Proteomics; D023822:Tissue Engineering
chemical_list: D003432:Cross-Linking Reagents; D011108:Polymers; D011506:Proteins