title: BMPRIB and BMPRII mRNA expression levels in goat ovarian follicles and the in vitro effects of BMP-15 on preantral follicle development.
abstract: This study evaluated the levels of bone morphogenetic protein receptors BMPRIB and BMPRII mRNA in goat follicles and the effects of bone morphogenetic protein-15 (BMP-15) on the in vitro development of cultured preantral follicles. Real-time polymerase chain reaction (PCR) was used to analyze the levels of BMPRIB and BMPRII mRNA in caprine preantral follicles and in small and large antral follicles. Preantral follicles (≥150 μm) were also isolated from goat ovaries and cultured for 18 days in α-MEM(+) supplemented with or without BMP-15 (10, 50, or 100 ng/ml). At the end of culture, some follicles were fixed for ultrastructural evaluation. Real-time PCR showed a reduction in BMPRII mRNA levels from the primary to secondary follicles. Higher levels of BMPRIB mRNA were observed in granulosa/theca cells from large antral follicles compared with small antral follicles. Moreover, BMPRII mRNA was expressed to a greater extent in cumulus-oocyte complexes from large antral follicles than in their respective granulosa/theca cells. In culture, 50 ng/ml BMP-15 positively influenced antral cavity formation and follicle growth after 18 days and also maintained follicular integrity. Thus, BMPRIB and BMPRII mRNAs are present in all follicular categories. BMP-15 (50 ng/ml) stimulates growth, antrum formation and the ultrastructural integrity of isolated caprine preantral follicles after 18 days of culture.
authors.affiliation: Faculty of Veterinary, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, CE, Brazil. dora.uece@gmail.com.
authors.forename: Isadora Machado T
authors.initials: IM
authors.lastname: Lima
authors.forename: Ivina R
authors.initials: IR
authors.lastname: Brito
authors.forename: Rafael
authors.initials: R
authors.lastname: Rossetto
authors.forename: Ana Beatriz G
authors.initials: AB
authors.lastname: Duarte
authors.forename: Giovanna Q
authors.initials: GQ
authors.lastname: Rodrigues
authors.forename: Márcia Viviane A
authors.initials: MV
authors.lastname: Saraiva
authors.forename: José Jackson N
authors.initials: JJ
authors.lastname: Costa
authors.forename: Mariana Aragão M
authors.initials: MA
authors.lastname: Donato
authors.forename: Christina A
authors.initials: CA
authors.lastname: Peixoto
authors.forename: José Roberto V
authors.initials: JR
authors.lastname: Silva
authors.forename: José Ricardo
authors.initials: JR
authors.lastname: de Figueiredo
authors.forename: Ana Paula R
authors.initials: AP
authors.lastname: Rodrigues
mesh_terms: D000818:Animals; D055430:Bone Morphogenetic Protein 15 / Q000494:pharmacology*; D052005:Bone Morphogenetic Protein Receptors, Type I / Q000235:genetics* / Q000378:metabolism; D052006:Bone Morphogenetic Protein Receptors, Type II / Q000235:genetics* / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D006041:Goats / Q000235:genetics; D006801:Humans; D008540:Meiosis / Q000187:drug effects; D008856:Microscopy, Fluorescence; D009865:Oocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006080:Ovarian Follicle / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism* / Q000648:ultrastructure; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D046509:Tissue Culture Techniques; D014022:Tissue Survival / Q000187:drug effects
chemical_list: D055430:Bone Morphogenetic Protein 15; D012333:RNA, Messenger; D052005:Bone Morphogenetic Protein Receptors, Type I; D052006:Bone Morphogenetic Protein Receptors, Type II