title: CD133+CD34+ and CD133+CD38+ blood progenitor cells as predictors of platelet engraftment in patients undergoing autologous peripheral blood stem cell transplantation.
abstract: BACKGROUND
  Hematopoietic stem cells (HSC) have been characterized by CD34+ expression and an adequate dose of CD34+ cells is associated with a complete engraftment. CD133 is a more specific marker of HSC.
  
  
  METHODS
  We studied the relationship between graft content of CD34+, CD133+, and CD38+ cells and trilineage engraftment after autologous stem cell transplantation in patients with different hematological disorders. Blood samples were obtained before and after mobilization with recombinant granulocyte-colony stimulating factor (G-CSF, 16 μg/kg), from apheresis collections, and after transplantation.
  
  
  RESULTS
  Cell subsets were quantified by flow cytometry, and the dose of each population infused was correlated with success of engraftment. G-CSF induced mobilization of CD133+CD38+ cells (12.6-fold) and CD133+CD34+ cells (14.7-fold). A correlation was observed between the infused dose of CD133+CD34+ and CD133+CD38+ cells and platelet engraftment.
  
  
  CONCLUSIONS
  CD133+CD34+ and CD133+CD38+ cells were mobilized with G-CSF and these cell subsets were correlated with platelet engraftment.
authors.affiliation: Departamento de Morfología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México, DF, Mexico.
authors.forename: Alma Y
authors.initials: AY
authors.lastname: Camacho Villa
authors.forename: Elba
authors.initials: E
authors.lastname: Reyes Maldonado
authors.forename: Laura A
authors.initials: LA
authors.lastname: Montiel Cervantes
authors.forename: Jorge
authors.initials: J
authors.lastname: Vela Ojeda
mesh_terms: D000071916:AC133 Antigen; D051997:ADP-ribosyl Cyclase 1; D000293:Adolescent; D000328:Adult; D015703:Antigens, CD; D018952:Antigens, CD34; D001792:Blood Platelets / Q000166:cytology* / Q000378:metabolism; D005260:Female; D006023:Glycoproteins; D006085:Graft Survival; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage*; D019650:Hematopoietic Stem Cell Mobilization; D006801:Humans; D008297:Male; D055016:Megakaryocyte Progenitor Cells / Q000166:cytology* / Q000378:metabolism; D008562:Membrane Glycoproteins; D008875:Middle Aged; D010455:Peptides; D036102:Peripheral Blood Stem Cell Transplantation; D014182:Transplantation, Autologous
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D018952:Antigens, CD34; D006023:Glycoproteins; D008562:Membrane Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; D016179:Granulocyte Colony-Stimulating Factor; C498154:CD38 protein, human; D051997:ADP-ribosyl Cyclase 1