title: [Expression of transcription factors T-bet and GATA-3 and relationship to the numbers of eosinophils in rat model of allergic rhinitis].
abstract: OBJECTIVE
  To explore the expression of T-bet/GATA-3 in nasal mucosa tissue of allergic rhinitis rat and to investigate the association between the expression of T-bet/GATA-3 and the eosinophil count.
  
  
  METHODS
  Twenty SD rats were randomly divided into a control group and an allergic rhinitis group. The allergic rhinitis rat model was induced with ovalbumin. The total eosinophils were counted in the nasal mucosa. The concentrations of IL-4, IL-5 and IFN-gamma in nasal lavage fluid were measured by ELISA. The mRNA and protein expressions of IL-4, IL-5, IFN-gamma, T-bet and GATA-3 in the nasal mucosa were detected by RT-PCR and Western blot respectively.
  
  
  RESULTS
  The main inflammatory cells were eosinophils in the nasal mucosa of allergic rhinitis rats. The level of IL-4, IL-5 and IFN-gamma in control group was significantly higher than that in allergic rhinitis group (P < 0.01). The mRNA and protein expression of IFN-gamma and T-bet in allergic rhinitis group was significantly higher than that in control group (P < 0.01). While the mRNA and protein expression of IL-4, IL-5 and GATA-3 in control group was significantly higher than that in allergic rhinitis group (P < 0.01). The ratio of protein expression of T-bet and GATA-3 was negatively correlated with the eosinophil count, IL-4 and IL-5, but positively with the concentrations of IFN-gamma.
  
  
  CONCLUSIONS
  The imbalance of transcription factor GATA-3 and T-bet has a close correlation with the eosinophil count, and may play a key role in the formation of allergic rhinitis.
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, the Second Affiliated Hospital, Nanchang University, Nanchang, 330006, China. artent@sina.com.cn
authors.forename: Chunping
authors.initials: C
authors.lastname: Yang
authors.forename: Chunlin
authors.initials: C
authors.lastname: Li
authors.forename: Gui
authors.initials: G
authors.lastname: Luo
authors.forename: Ying
authors.initials: Y
authors.lastname: Luo
authors.forename: Yuehui
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000818:Animals; D002452:Cell Count; D004804:Eosinophils / Q000166:cytology*; D005260:Female; D050990:GATA3 Transcription Factor / Q000378:metabolism*; D006967:Hypersensitivity / Q000276:immunology / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012220:Rhinitis / Q000276:immunology / Q000378:metabolism*; D020825:T-Box Domain Proteins / Q000378:metabolism*; D018417:Th1 Cells / Q000378:metabolism; D018418:Th2 Cells / Q000378:metabolism; D000098815:T-bet Transcription Factor
chemical_list: D050990:GATA3 Transcription Factor; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor