title: DNA-directed three-dimensional protein organization.
abstract: All bound together: self-assembled symmetric DNA polyhedra were used to organize proteins in 3D space. Biotin moieties were incorporated into the self-assembled symmetric DNA polyhedra. Upon incubation with streptavidin (STV) protein, an STV protein became bound to each polyhedral face, thus resulting in well-structured DNA polyhedra/STV complexes. This strategy was also applied to different 3D DNA nanostructures and different proteins.
authors.affiliation: Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA.
authors.forename: Chuan
authors.initials: C
authors.lastname: Zhang
authors.forename: Cheng
authors.initials: C
authors.lastname: Tian
authors.forename: Fei
authors.initials: F
authors.lastname: Guo
authors.forename: Zheng
authors.initials: Z
authors.lastname: Liu
authors.forename: Wen
authors.initials: W
authors.lastname: Jiang
authors.forename: Chengde
authors.initials: C
authors.lastname: Mao
mesh_terms: D000906:Antibodies / Q000276:immunology; D001710:Biotin / Q000737:chemistry / Q000378:metabolism; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism; D049329:Nanostructures / Q000737:chemistry; D019809:Streptavidin / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000906:Antibodies; D055767:Immobilized Proteins; D001710:Biotin; D004247:DNA; D019809:Streptavidin