title: [Effects of interleukin-17 on murine pulmonary fibroblast proliferation, transformation and collagen synthesis].
abstract: OBJECTIVE
  To investigate the effects of interleukin-17 (IL-17) on the proliferation, transformation and collagen synthesis of the lung fibroblasts in mice with bleomycin-induced pulmonary fibrosis.
  
  
  METHODS
  In a mouse model of pulmonary fibrosis established by intratracheal administration of 5 mg/kg bleomycin, the dynamic expressions of IL-17/IL-17 receptor (IL-17R) mRNAs were detected by RT-PCR. At 14 days following bleomycin administration, the pulmonary fibroblasts were isolated, cultured and identified. MTT assay was used to assess the proliferation of the pulmonary fibroblasts in response to IL-17 treatment at different concentrations, and RT-PCR and Western blotting were employed to examine the mRNA and protein expressions of α-smooth muscle actin (α-SMA) and types I and III collagen.
  
  
  RESULTS
  IL-17/IL-17R mRNA levels were increased obviously in the pulmonary fibroblasts of rats with pulmonary fibrosis, and the highest expressions occurred at 14 days following bleomycin administration. Exogenous IL-17, at the optimal concentration of 50 ng/ml, significantly promoted the proliferation of the pulmonary fibroblasts in primary culture and obviously increased α-SMA expression and types I and III collagen synthesis in the fibroblasts.
  
  
  CONCLUSIONS
  IL-17 can promote the proliferation, transformation, and collagen synthesis of the pulmonary fibroblasts from rats with bleomycin-induced pulmonary fibrosis.
authors.affiliation: Department of Respiratory, Kunming Medical University, Kunming, China. dongkm@hotmail.com
authors.forename: Zhaoxing
authors.initials: Z
authors.lastname: Dong
authors.forename: Qingxin
authors.initials: Q
authors.lastname: Kang
authors.forename: Wen
authors.initials: W
authors.lastname: Lei
authors.forename: Hong
authors.initials: H
authors.lastname: Zhong
authors.forename: Wenlin
authors.initials: W
authors.lastname: Tai
authors.forename: Dianhua
authors.initials: D
authors.lastname: Wang
mesh_terms: D000818:Animals; D001761:Bleomycin; D049109:Cell Proliferation; D002478:Cells, Cultured; D024042:Collagen Type I / Q000096:biosynthesis*; D024061:Collagen Type III / Q000096:biosynthesis; D058750:Epithelial-Mesenchymal Transition; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology*; D020381:Interleukin-17 / Q000235:genetics / Q000378:metabolism*; D008168:Lung / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011658:Pulmonary Fibrosis / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D053722:Receptors, Interleukin-17 / Q000235:genetics / Q000378:metabolism
chemical_list: D024042:Collagen Type I; D024061:Collagen Type III; D020381:Interleukin-17; D012333:RNA, Messenger; D053722:Receptors, Interleukin-17; D001761:Bleomycin