title: Electrospun polyvinyl alcohol-collagen-hydroxyapatite nanofibers: a biomimetic extracellular matrix for osteoblastic cells.
abstract: The failure of prosthesis after total joint replacement is due to the lack of early implant osseointegration. In this study polyvinyl alcohol-collagen-hydroxyapatite (PVA-Col-HA) electrospun nanofibrous meshes were fabricated as a biomimetic bone-like extracellular matrix for the modification of orthopedic prosthetic surfaces. In order to reinforce the PVA nanofibers, HA nanorods and Type I collagen were incorporated into the nanofibers. We investigated the morphology, biodegradability, mechanical properties and biocompatibility of the prepared nanofibers. Our results showed these inorganic-organic blended nanofibers to be degradable in vitro. The encapsulated nano-HA and collagen interacted with the PVA content, reinforcing the hydrolytic resistance and mechanical properties of nanofibers that provided longer lasting stability. The encapsulated nano-HA and collagen also enhanced the adhesion and proliferation of murine bone cells (MC3T3) in vitro. We propose the PVA-Col-HA nanofibers might be promising modifying materials on implant surfaces for orthopedic applications.
authors.affiliation: Department of Biomedical Engineering, Wayne State University, Detroit, MI, USA. as7606@wayne.edu
authors.forename: Wei
authors.initials: W
authors.lastname: Song
authors.forename: David C
authors.initials: DC
authors.lastname: Markel
authors.forename: Sunxi
authors.initials: S
authors.lastname: Wang
authors.forename: Tong
authors.initials: T
authors.lastname: Shi
authors.forename: Guangzhao
authors.initials: G
authors.lastname: Mao
authors.forename: Weiping
authors.initials: W
authors.lastname: Ren
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis; D040761:Biomimetic Materials / Q000138:chemical synthesis*; D018786:Bone Substitutes / Q000138:chemical synthesis; D002448:Cell Adhesion; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D024042:Collagen Type I / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D005109:Extracellular Matrix / Q000737:chemistry*; D051379:Mice; D057139:Nanofibers / Q000737:chemistry*; D010006:Osteoblasts; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D024042:Collagen Type I; D011142:Polyvinyl Alcohol; D017886:Durapatite