title: A rapid microtiter plate serum bactericidal assay method for determining serum complement-mediated killing of Mannheimia haemolytica.
abstract: In this study, we describe a rapid microtiter serum bactericidal assay (RMSBA) that can be used to measure the functionality of immune sera. It quantifies bactericidal activity of immune sera in the presence of complement against a homologous bacterium, M. haemolytica in this case. There is high correlation between data from RMSBA and standard complement-mediated bacterial killing assay (r=0.756; p<0.0001). The RMSBA activity of sera can be generated in less than 5 h instead of overnight incubation. RMSBA costs substantially less in terms of time, labor, and resources and is highly reproducible.
authors.affiliation: Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Stillwater, Oklahoma 74078, USA. sahlu.ayalew@okstate.edu
authors.forename: Sahlu
authors.initials: S
authors.lastname: Ayalew
authors.forename: Anthony W
authors.initials: AW
authors.lastname: Confer
authors.forename: Binu
authors.initials: B
authors.lastname: Shrestha
authors.forename: Mark E
authors.initials: ME
authors.lastname: Payton
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood*; D001431:Bacteriological Techniques / Q000379:methods*; D001770:Blood Bactericidal Activity; D003165:Complement System Proteins / Q000276:immunology*; D016978:Mannheimia haemolytica / Q000276:immunology*; D015203:Reproducibility of Results; D044967:Serum / Q000276:immunology*; D013997:Time Factors
chemical_list: D000907:Antibodies, Bacterial; D003165:Complement System Proteins