title: Identification and characterization of zebrafish SULT1 ST9, SULT3 ST4, and SULT3 ST5.
abstract: By searching the GenBank database, we identified sequences encoding three new zebrafish cytosolic sulfotransferases (SULTs). These three new zebrafish SULTs, designated SULT1 ST9, SULT3 ST4, and SULT3 ST5, were cloned, expressed, purified, and characterized. SULT1 ST9 appeared to be mostly involved in the metabolism and detoxification of xenobiotics such as β-naphthol, β-naphthylamine, caffeic acid and gallic acid. SULT3 ST4 showed strong activity toward endogenous compounds such as dehydroepiandrosterone (DHEA), pregnenolone, and 17β-estradiol. SULT3 ST5 showed weaker, but significant, activities toward endogenous compounds such as DHEA and corticosterone, as well as xenobiotics including mestranol, β-naphthylamine, β-naphthol, and butylated hydroxyl anisole (BHA). pH-dependency and kinetic constants of these three enzymes were determined with DHEA, β-naphthol, and 17β-estradiol as substrates. Reverse transcription-polymerase chain reaction (RT-PCR) was performed to examine the expression of these three new zebrafish SULTs at different developmental stages during embryogenesis, through larval development, and on to maturity.
authors.affiliation: Department of Pharmacology, College of Pharmacy, The University of Toledo, Toledo, OH 43614, USA.
authors.forename: Yasir I
authors.initials: YI
authors.lastname: Mohammed
authors.forename: Katsuhisa
authors.initials: K
authors.lastname: Kurogi
authors.forename: Amani Al
authors.initials: AA
authors.lastname: Shaban
authors.forename: Zheng
authors.initials: Z
authors.lastname: Xu
authors.forename: Ming-Yih
authors.initials: MY
authors.lastname: Liu
authors.forename: Frederick E
authors.initials: FE
authors.lastname: Williams
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Sakakibara
authors.forename: Masahito
authors.initials: M
authors.lastname: Suiko
authors.forename: Shakhawat
authors.initials: S
authors.lastname: Bhuiyan
authors.forename: Ming-Cheh
authors.initials: MC
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016000:Cluster Analysis; D003600:Cytosol / Q000201:enzymology; D018507:Gene Expression Regulation, Developmental; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008969:Molecular Sequence Data; D011485:Protein Binding; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D013379:Substrate Specificity; D015238:Sulfotransferases / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D015027:Zebrafish / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D029961:Zebrafish Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D029961:Zebrafish Proteins; D015238:Sulfotransferases