title: Culture of neonatal rodent microglia, astrocytes, and oligodendrocytes from cortex and spinal cord.
abstract: The protocol described in this chapter covers the preparation and culture of enriched populations of microglia, astrocytes, and oligodendrocytes from the cortex and spinal cord of neonatal rat and mouse. The procedure is based on the enzymatic digestion of tissue, followed by the culture of a mixed glial cell population which is then utilized as the starting point for the isolation, via differential attachment, of the different cell types.
authors.affiliation: Department of Pharmacology and Anesthesiology, University of Padova, Padova, Italy. stephen.skaper@unipd.it
authors.forename: Stephen D
authors.initials: SD
authors.lastname: Skaper
authors.forename: Carla
authors.initials: C
authors.lastname: Argentini
authors.forename: Massimo
authors.initials: M
authors.lastname: Barbierato
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000166:cytology*; D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation / Q000379:methods*; D002540:Cerebral Cortex / Q000166:cytology*; D051379:Mice; D017628:Microglia / Q000166:cytology*; D009836:Oligodendroglia / Q000166:cytology*; D051381:Rats; D013116:Spinal Cord / Q000166:cytology*