title: Mitochondrial proteome analysis reveals depression of the Ndufs3 subunit and activity of complex I in diabetic rat brain.
abstract: Type-1 diabetes resulting from defective insulin secretion and consequent hyperglycemia, is associated with "diabetic encephalopathy." This is characterized by brain neurophysiological and structural changes resulting in impairment of cognitive function. The present proteomic analysis of brain mitochondrial proteins from streptozotocin-induced type-1 diabetic rats, shows a large decrement of the Ndufs3 protein subunit of complex I, decreased level of the mRNA and impaired catalytic activity of the complex in the diabetic rats as compared to controls. The severe depression of the expression and enzymatic activity of complex I can represent a critical contributing factor to the onset of the diabetic encephalopathy in type-1 diabetes.
authors.affiliation: Department of Medical Basic Sciences, University of Bari Aldo Moro, Bari, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Taurino
authors.forename: Eleonora
authors.initials: E
authors.lastname: Stanca
authors.forename: Luisa
authors.initials: L
authors.lastname: Siculella
authors.forename: Raffaella
authors.initials: R
authors.lastname: Trentadue
authors.forename: Sergio
authors.initials: S
authors.lastname: Papa
authors.forename: Franco
authors.initials: F
authors.lastname: Zanotti
authors.forename: Antonio
authors.initials: A
authors.lastname: Gnoni
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D001923:Brain Chemistry / Q000502:physiology; D001928:Brain Diseases, Metabolic / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D003922:Diabetes Mellitus, Type 1 / Q000139:chemically induced / Q000150:complications / Q000378:metabolism / Q000473:pathology; D015536:Down-Regulation; D042967:Electron Transport Complex I / Q000032:analysis / Q000378:metabolism*; D004789:Enzyme Activation; D008297:Male; D024101:Mitochondrial Proteins / Q000032:analysis* / Q000378:metabolism; D008954:Models, Biological; D009245:NADH Dehydrogenase / Q000032:analysis / Q000378:metabolism*; D021122:Protein Subunits / Q000032:analysis / Q000378:metabolism; D020543:Proteome / Q000032:analysis / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin
chemical_list: D024101:Mitochondrial Proteins; D021122:Protein Subunits; D020543:Proteome; D013311:Streptozocin; D009245:NADH Dehydrogenase; D042967:Electron Transport Complex I; C472512:NDUFS3 protein, human