title: Urinary clusterin as a renal marker in dogs.
abstract: A validation of a species-specific enzyme immunoassay for urinary clusterin measurement in dogs was performed, and the use of urinary clusterin as a marker of renal damage was evaluated in a population of dogs with leishmaniasis. Urine was obtained from 75 dogs; 64 dogs had leishmaniasis and 11 were healthy. The dogs with leishmanias were divided into 5 groups: I (n = 9; serum creatinine [SCr] < 1.4 mg/dl, urinary protein-to-creatinine [UPC] ratio ≤ 0.5); II (n = 29; SCr < 1.4 mg/dl, UPC > 0.5); III (n = 6; SCr ≥ 1.4 mg/dl to <2 mg/dl, UPC > 0.5); IV (n = 13; SCr ≥ 2 mg/dl to <5 mg/dl, UPC > 0.5); and V (n = 7; SCr ≥ 5 mg/dl, UPC > 0.5). The urinary clusterin concentration was measured, and the urinary clusterin-to-creatinine ratio was calculated. Canine urinary clusterin assay showed good analytical performance based on precision accuracy and limit-of-detection results. There was a statistically significant increase in urinary clusterin and clusterin-to-creatinine ratio in groups II-V compared with group I and healthy group. The results of the current study showed that urinary clusterin concentration and urinary clusterin-to-creatinine ratios are increased in dogs with analytical evidences of renal damage and that the urinary clusterin-to-creatinine ratio might be used as a potential early biomarker of chronic kidney disease.
authors.affiliation: Department of Animal Surgery and Medicine, Faculty of Veterinary Medicine, University of Murcia, Espinardo, Murcia, Spain. juandi@um.es
authors.forename: Juan D
authors.initials: JD
authors.lastname: García-Martínez
authors.forename: Asta
authors.initials: A
authors.lastname: Tvarijonaviciute
authors.forename: José J
authors.initials: JJ
authors.lastname: Cerón
authors.forename: Marco
authors.initials: M
authors.lastname: Caldin
authors.forename: Silvia
authors.initials: S
authors.lastname: Martínez-Subiela
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000652:urine; D051152:Clusterin / Q000652:urine*; D003404:Creatinine / Q000652:urine; D004283:Dog Diseases / Q000378:metabolism / Q000469:parasitology* / Q000652:urine*; D004285:Dogs; D005260:Female; D007124:Immunoenzyme Techniques / Q000662:veterinary; D007674:Kidney Diseases / Q000378:metabolism / Q000469:parasitology / Q000652:urine / Q000662:veterinary*; D007891:Leishmania / Q000302:isolation & purification*; D007896:Leishmaniasis / Q000378:metabolism / Q000469:parasitology / Q000652:urine / Q000662:veterinary*; D057230:Limit of Detection; D008297:Male; D018709:Statistics, Nonparametric
chemical_list: D015415:Biomarkers; D051152:Clusterin; D003404:Creatinine