title: [Preparation and evaluation of amylose and cellulose tris (3-trifluoromethylphenylcarbamates)-based chiral stationary phases].
abstract: To broaden the category of polysaccharide-based chiral stationary phases (CSPs), coated CSPs based on tris (3-trifluoromethylphenylcarbamates) of amylose and cellulose were prepared for enantioseparation in high performance liquid chromatography. Their performances were evaluated by eight racemates using hexane-isopropanol as mobile phase. Compared with the most widely used, commercially available polysaccharide-based CSPs, Chiralpak AD and Chiralcel OD, utilizing tris (3,5-dimethylphenylcarbamates) of amylose and cellulose as the chiral selector, respectively, the obtained CSPs exhibited lower enantioseparation abilities. However, cellulose tris(3-trifluoromethylphenylcarbamate)-based CSP exhibited characteristic enantioseparation and some chiral compounds were better resolved on this CSP than on Chiral-cel OD. The chiral recognition abilities of the obtained CSPs were increased with the decrease of the portion of isopropanol in the mobile phase and relatively high enantioseparation was obtained with the mobile phase of hexane-isopropanol (95: 5, v/v). The obtained amylosic phase demonstrated slightly higher chiral resolving ability than the cellulosic one for the test racemates. In addition, it was revealed that the enantioseparations of the cellulosic and amylosic CSPs were complementary.
authors.affiliation: College of Chemical Engineering and Materials Science, Zhejiang University of Technology, Hangzhou 31001 China.
authors.forename: Zhaolei
authors.initials: Z
authors.lastname: Jin
authors.forename: Fangfang
authors.initials: F
authors.lastname: Hu
authors.forename: Yibo
authors.initials: Y
authors.lastname: Wang
authors.forename: Guihua
authors.initials: G
authors.lastname: Liu
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Fuyou
authors.initials: F
authors.lastname: Pan
authors.forename: Shouwan
authors.initials: S
authors.lastname: Tang
mesh_terms: D000688:Amylose / Q000737:chemistry*; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D048448:Phenylcarbamates / Q000737:chemistry*; D013237:Stereoisomerism
chemical_list: D048448:Phenylcarbamates; C065943:cellulose tris-3,5-dimethylphenyl-carbamate; D002482:Cellulose; D000688:Amylose