title: Identification of ITGB4BP as a new interaction protein of P311.
abstract: OBJECTIVE
  P311 is an 8 kDa protein that has been shown to be of importance in the process of myofibroblast transformation, glioblastoma invasion and nerve regeneration. However, the interaction protein of P311 has yet to be found. The purpose of this study was to find the interactive protein of P311.
  
  
  METHODS
  The yeast two-hybrid system was used for screening the potential interaction proteins of P311. Joint expression of the potential interactive protein and P311 was immunohistochemically stained. The interaction between P311 and the selected protein was further confirmed by fluorescence resonance energy transfer (FRET) in pulmonary adenocarcinoma tissue sections, and by coimmunoprecipitation in HEK293.
  
  
  RESULTS
  Integrin β4 binding protein (ITGB4BP) was confirmed as the interaction protein of P311. Co-expression and interaction of ITGB4BP and P311 were demonstrated in pulmonary adenocarcinoma by both immunohistochemistry and FRET. Moreover the interaction between P311 and ITGB4BP was demonstrated by coimmunoprecipitation in HEK293.
  
  
  CONCLUSIONS
  The interactions between P311 and ITGB4BP may be very important in the process of tumor cell differentiation and metastasis. ITGB4BP may provide a potential new target for the therapy of tumors.
authors.affiliation: Institute of Burn Research, State Key Laboratory of Trauma, Burns and Combined Injury, Department of Pathology, Southwest Hospital, Third Military Medical University, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Peng
authors.forename: Shunzong
authors.initials: S
authors.lastname: Yuan
authors.forename: Jianglin
authors.initials: J
authors.lastname: Tan
authors.forename: Bing
authors.initials: B
authors.lastname: Ma
authors.forename: Xiuwu
authors.initials: X
authors.lastname: Bian
authors.forename: Chengping
authors.initials: C
authors.lastname: Xu
authors.forename: Weifeng
authors.initials: W
authors.lastname: He
authors.forename: Hongwei
authors.initials: H
authors.lastname: Cao
authors.forename: Zhenggen
authors.initials: Z
authors.lastname: Huang
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Cui
authors.forename: Chengjun
authors.initials: C
authors.lastname: Gan
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Wang
authors.forename: Junyi
authors.initials: J
authors.lastname: Zhou
authors.forename: Jie
authors.initials: J
authors.lastname: Hu
authors.forename: Sisi
authors.initials: S
authors.lastname: Yang
authors.forename: Gaoxing
authors.initials: G
authors.lastname: Luo
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism*; D039642:Eukaryotic Initiation Factors / Q000378:metabolism*; D031541:Fluorescence Resonance Energy Transfer; D019084:Fluorescent Antibody Technique, Indirect; D057809:HEK293 Cells; D006801:Humans; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D008175:Lung Neoplasms / Q000378:metabolism*; D018613:Microscopy, Confocal; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D015513:Oncogene Proteins / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
chemical_list: C109284:EIF6 protein, human; D039642:Eukaryotic Initiation Factors; C428027:NREP protein, human; D009419:Nerve Tissue Proteins; D015513:Oncogene Proteins