title: Measurement of MMP Activity in Synovial Fluid in Cases of Osteoarthritis and Acute Inflammatory Conditions of the Knee Joints Using a Fluorogenic Peptide Probe-Immobilized Diagnostic Kit.
abstract: OBJECTIVE
  A fluorogenic peptide probe-immobilized diagnostic kit was used to analyze MMP activity in the synovial fluids (SFs) from patients with osteoarthritis (OA) and acute inflammatory conditions of the knee joint.
  
  
  METHODS
  The MMP diagnostic kit containing a polymer-conjugated MMP probe immobilized on a 96-well plate was utilized for high-throughput screening of MMP activity in SFs from OA patients (n = 33) and patients with acute inflammatory conditions of the knee joint (n = 5).
  
  
  RESULTS
  Compared to SF from OA patients, SF from patients with acute inflammatory conditions of the knee joint presented stronger NIR fluorescent signals. In gelatin zymography, most samples from patients with acute inflammatory conditions of the knee joint also displayed 92 kDa (pro-form) MMP-9 and faint 84 kDa (active form) MMP-9, while SF from OA patients did not display detectable MMP-9 activity .
  
  
  CONCLUSIONS
  The presence of a strong fluorescence signal from the MMP diagnostic kit corresponded well with patients with acute inflammatory conditions of the knee joint. The results suggest that our MMP diagnostic kit can be useful in differentiation between early stages of OA and acute inflammatory conditions of the knee joint.
authors.affiliation: 1. Biomedical Research Center, Korea Institute of Science and Technology, Seongbuk-Gu, Seoul, South Korea;
authors.forename: Ju Hee
authors.initials: JH
authors.lastname: Ryu
authors.forename: Aeju
authors.initials: A
authors.lastname: Lee
authors.forename: Myung Sook
authors.initials: MS
authors.lastname: Huh
authors.forename: Junuk
authors.initials: J
authors.lastname: Chu
authors.forename: Kwangmeyung
authors.initials: K
authors.lastname: Kim
authors.forename: Byung-Soo
authors.initials: BS
authors.lastname: Kim
authors.forename: Kuiwon
authors.initials: K
authors.lastname: Choi
authors.forename: Ick Chan
authors.initials: IC
authors.lastname: Kwon
authors.forename: Jong Woong
authors.initials: JW
authors.lastname: Park
authors.forename: Inchan
authors.initials: I
authors.lastname: Youn