title: A poly(L-lysine)-based hydrophilic star block co-polymer as a protein nanocarrier with facile encapsulation and pH-responsive release.
abstract: A hydrophilic star block co-polymer was synthesized, characterized, and evaluated as a protein nanocarrier. The star block co-polymer was composed of a hyperbranched polyethylenimine (PEI) core, a poly(L-lysine) (PLL) inner shell, and a poly(ethylene glycol) (PEG) outer shell. The model protein insulin can be rapidly and efficiently encapsulated by the synthesized polymer in aqueous phosphate buffer at physiological pH. Complexation between PEI-PLL-b-PEG and insulin was investigated using native polyacrylamide gel electrophoresis. The uptake of enhanced green fluorescent protein into Ad293 cells mediated by PEI-PLL-b-PEG was also investigated. The encapsulated insulin demonstrated sustained release at physiological pH and showed accelerated release when the pH was decreased. The insulin released from the star block co-polymer retained its chemical integrity and immunogenicity.
authors.affiliation: Medical College, Shantou University, Shantou 515041, People's Republic of China.
authors.forename: Yunsong
authors.initials: Y
authors.lastname: Yan
authors.forename: Daixu
authors.initials: D
authors.lastname: Wei
authors.forename: Jiayan
authors.initials: J
authors.lastname: Li
authors.forename: Jinhong
authors.initials: J
authors.lastname: Zheng
authors.forename: Ganggang
authors.initials: G
authors.lastname: Shi
authors.forename: Wenhong
authors.initials: W
authors.lastname: Luo
authors.forename: Ying
authors.initials: Y
authors.lastname: Pan
authors.forename: Jinzhi
authors.initials: J
authors.lastname: Wang
authors.forename: Lumian
authors.initials: L
authors.lastname: Zhang
authors.forename: Xiaoying
authors.initials: X
authors.lastname: He
authors.forename: Daojun
authors.initials: D
authors.lastname: Liu
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004337:Drug Carriers; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D007328:Insulin / Q000008:administration & dosage; D009682:Magnetic Resonance Spectroscopy; D011107:Polylysine / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011506:Proteins / Q000008:administration & dosage*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D004337:Drug Carriers; D007328:Insulin; D011108:Polymers; D011506:Proteins; D011107:Polylysine