title: [Clinical application of DNA sequencing for detecting point mutations in hepatitis B virus associated with drug resistance].
abstract: OBJECTIVE
  [corrected] To assess the specificity and applicability of direct PCR sequencing in the detection of point mutations in hepatitis B virus (HBV) associated with drug resistance.
  
  
  METHODS
  Serum samples were obtained from 120 patients with hepatitis B treated with nucleoside analogus for at least 2 years to detect the point mutations in HBV genome in association with drug resistance using nested PCR and direct DNA sequencing.
  
  
  RESULTS
  Forty out of the 120 patients were found to have one or two point mutations associated with drug resistance, including 17 with L180M and M204V/I mutations (42.5%), 10 with M204V/I mutation (25%), 8 with N236T mutation (20%), 3 with L180M mutation (7.5%), and 1 with both A181V/T and N236T mutations (2.5%), and 1 with A181V/T mutation(2.5%).
  
  
  CONCLUSIONS
  DNA sequencing is a good method to detect all known point mutations associated with HBV drug resistance.
authors.affiliation: Jiujiang Third People's Hospital, Jiujiang, China. konglinghe8882@163.com
authors.forename: Linghe
authors.initials: L
authors.lastname: Kong
authors.forename: Suxiang
authors.initials: S
authors.lastname: Gao
authors.forename: Yunxia
authors.initials: Y
authors.lastname: Ke
authors.forename: Jian
authors.initials: J
authors.lastname: Yang
authors.forename: Guiping
authors.initials: G
authors.lastname: Lei
authors.forename: Guoxiang
authors.initials: G
authors.lastname: Shi
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D024882:Drug Resistance, Viral / Q000235:genetics*; D005260:Female; D006515:Hepatitis B virus / Q000187:drug effects / Q000235:genetics*; D019694:Hepatitis B, Chronic / Q000188:drug therapy / Q000821:virology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009705:Nucleosides / Q000627:therapeutic use*; D017354:Point Mutation / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D055815:Young Adult
chemical_list: D009705:Nucleosides