title: [Construction and expression of recombinant adenovirus encoding MDA-7/IL-24 and its inhibitory effect on proliferation in human hepatocellular carcinoma cells].
abstract: OBJECTIVE
  To construct recombinant plasmid and adenovirus harboring MDA-7 gene, and to investigate its biological function on the proliferation of human hepatocellular carcinoma cells.
  
  
  METHODS
  The MDA-7 fragments from the T vectors were inserted into pCDNA-3 vector to construct expression plasmids named pCDNA3-MDA-7. To determine its effects on the proliferation of HCC cells, transfected the expression vector into cells and tested the ability of colony formation in cancer cells. Simultaneously, constructed recombinant adenovirus expressing MDA-7, and detected its effect on the proliferation of HCC cells by using MTT assay.
  
  
  RESULTS
  Successfully constructed plasmid- and adenovirus-based system to express MDA-7. The data of colony formation assay and M'T test showed that MDA-7 can obviously suppress cell growth in HCC cells.
  
  
  CONCLUSIONS
  MDA-7 may function as tumor suppressor in HCC cells, and the adenovirus-mediated MDA-7 can be a novel strategy for the anti-HCC therapy. Our study established the foundation for future research on the effect of MDA-7 in HCC.
authors.affiliation: State Key Laboratory of Center Biology, Fourth Military Medical University, Xi'an 710032, China. biozx@fmmu.edu.cn
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhang
authors.forename: Yan-ling
authors.initials: YL
authors.lastname: Meng
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.forename: Bo
authors.initials: B
authors.lastname: Yan
authors.forename: Rui
authors.initials: R
authors.lastname: Cenh
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.forename: An-gang
authors.initials: AG
authors.lastname: Yang
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D006528:Carcinoma, Hepatocellular / Q000473:pathology / Q000628:therapy*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D007378:Interleukins / Q000235:genetics* / Q000502:physiology; D008113:Liver Neoplasms / Q000473:pathology / Q000628:therapy*; D011995:Recombination, Genetic; D000099180:Interleukin-24
chemical_list: D007378:Interleukins; D000099180:Interleukin-24