title: 8-Methoxypsoralen plus UVA treatment increases the proportion of CLA+ CD25+ CD4+ T cells in lymph nodes of K5.hTGFβ1 transgenic mice.
abstract: 8-Methoxypsoralen plus UVA (PUVA) photochemotherapy is an effective treatment for many skin diseases including psoriasis. However, its exact mechanism of therapeutic action is incompletely understood. Previously, in K5.hTGFβ1 transgenic psoriatic mice, we found that PUVA induces Foxp3+ CD25+ CD4+ regulatory T cells in both lymph node and spleen. Now, in the same model, we investigated whether cutaneous lymphocyte-associated antigen (CLA) mediates PUVA's effect on homing of CD25+ CD4+  T cells to the lymph nodes of K5.hTGFβ1 transgenic mice. We found that a low dose of topical PUVA maximally increased the proportion of CLA + CD25+ CD4 + T cells in the lymph nodes by up to 8-fold. We also observed an increased number of Foxp3+ CD25+  T cells in the skin of the mice after PUVA treatment. Together, these findings suggest that PUVA affects the homing of regulatory T cells.
authors.forename: Tej Pratap
authors.initials: TP
authors.lastname: Singh
authors.forename: Michael P
authors.initials: MP
authors.lastname: Schön
authors.forename: Katrin
authors.initials: K
authors.lastname: Wallbrecht
authors.forename: Peter
authors.initials: P
authors.lastname: Wolf
mesh_terms: D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000187:drug effects / Q000528:radiation effects; D004195:Disease Models, Animal; D053645:Interleukin-2 Receptor alpha Subunit / Q000187:drug effects / Q000528:radiation effects; D008198:Lymph Nodes / Q000166:cytology* / Q000187:drug effects / Q000528:radiation effects; D008730:Methoxsalen / Q000008:administration & dosage*; D051379:Mice; D008822:Mice, Transgenic; D011701:PUVA Therapy; D017319:Photosensitizing Agents / Q000008:administration & dosage*; D011565:Psoriasis / Q000188:drug therapy* / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects / Q000528:radiation effects
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; D053645:Interleukin-2 Receptor alpha Subunit; D017319:Photosensitizing Agents; D008730:Methoxsalen