title: A new fluorescent staining method for callose of microspore mother cells during meiosis.
abstract: To observe the dynamic behavior of callose of microspore mother cells during meiosis, we developed a convenient, rapid and efficient staining method using an improved carbol fuchsin/aniline blue solution. The stained microspore mother cells during meiosis showed yellowish green callose, red cytoplasm and dark red chromosomes when excited with blue light, which produced a contrasting image with a three-dimensional effect. When stained with only improved carbol fuchsin solution, the cells had red cytoplasm and chromosomes when excited with green light. The improved carbol fuchsin solution can be used to replace other more expensive DNA-specific dyes, such as DAPI and H33258, to reduce experimental costs.
authors.affiliation: College of Life Science, Capital Normal University, Beijing, People's Republic of China.
authors.forename: M
authors.initials: M
authors.lastname: Wang
authors.forename: L
authors.initials: L
authors.lastname: Wan
authors.forename: H
authors.initials: H
authors.lastname: Chen
authors.forename: X
authors.initials: X
authors.lastname: Liu
authors.forename: J
authors.initials: J
authors.lastname: Liu
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D005936:Glucans / Q000737:chemistry; D008540:Meiosis / Q000502:physiology*; D011058:Pollen / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism; D012394:Rosaniline Dyes / Q000737:chemistry; D013194:Staining and Labeling / Q000379:methods*; D013997:Time Factors
chemical_list: D000814:Aniline Compounds; D005456:Fluorescent Dyes; D005936:Glucans; D012394:Rosaniline Dyes; C006898:carbol-fuchsin solution; C017006:aniline blue; C048306:callose