title: [Determination of three organic acids in Schisandrae Chinensis Fructus by HPLC].
abstract: OBJECTIVE
  To establish an HPLC method for determination of three organic acids in Schisandrae Chinensis Fructus.
  
  
  METHODS
  The separation was performed on a C18 column with acetonitrile-15 mmol x L(-1) KH2 PO4 (1:99) and methanol-0.5% acetic glacial as the mobile phrase to determine the content of kinic acid, citric acid, and protocatechuic acid.
  
  
  RESULTS
  Three kinds of organic acid can be detective in different samples, and the average content of the three organic acids were about 18%, among which the citric acid was the highest (18%).
  
  
  CONCLUSIONS
  Since organic acids is an important parts of the material basis in Schisandrae Chinensis Fructus, it is necessary to carry out the pharmacological research associated with the function of Schisandrae Chinensis Fructus. Further, it will provide scientific basis for the drug relevance research between material basis and efficiency, as well as the foundation for the relevance research on the properties and Five Tastes of traditional Chinese medicine.
authors.affiliation: Institute of Chinese Mateia Madica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Yongqing
authors.initials: Y
authors.lastname: Xiao
authors.forename: Dingrong
authors.initials: D
authors.lastname: Yu
authors.forename: Yinlian
authors.initials: Y
authors.lastname: Ma
authors.forename: Wenqian
authors.initials: W
authors.lastname: Huang
authors.forename: Guofang
authors.initials: G
authors.lastname: Tian
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
mesh_terms: D001688:Biological Products / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D019343:Citric Acid / Q000032:analysis*; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D062385:Hydroxybenzoates / Q000032:analysis*; D032141:Schisandra / Q000737:chemistry*
chemical_list: D001688:Biological Products; D004365:Drugs, Chinese Herbal; D062385:Hydroxybenzoates; D019343:Citric Acid; C009091:protocatechuic acid