title: The significance of microthrombosis and fgl2 in no-reflow phenomenon of rats with acute myocardial ischemia/reperfusion.
abstract: No-reflow phenomenon due to cardiac microvascular dysfunction or disturbance aggravates clinic outcomes of a portion of patients with acute myocardial infarction undergoing percutaneous coronary intervention or thrombolytic therapy. Our working hypothesis was that cardiac microthrombosis would play an important role in the pathogenesis. We investigated that cardiac microthrombi were observed by Martius, Scarlet, Blue methocl (MSB) and Masson trichrome staining. Furthermore, we investigated the expression of fibrinogen-like protein 2 (fgl2) in rats with acute myocardial ischemia/reperfusion (MI/R) and its possible pathological and clinical significance. The fgl2 was highly expressed in myocardium of rats with acute MI/R and located at cardiac microvascular walls. We found that the expression of fgl2 in peripheral mononuclear cells of rats with acute MI/R significantly increased correspondingly with its cardiac expression. Expression of cardiac fgl2 was correlated with no-reflow size of rats with acute MI/R, which was detected and calculated by thioflavin S staining. No-reflow size was in line with cardiac diastolic dysfunction of rats with acute MI/R monitored by hemodynamics. Thus, microthrombosis is involved in cardiac microvascular dysfunction or disturbance of rats with acute MI/R as one cause, and fgl2 may emerge as a predictor of the occurrence of no-reflow phenomenon.
authors.affiliation: Department of Cardiology, Union Hospital of Huazhong, University of Science and Technology, Wuhan, Hubei Province, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Shao
authors.forename: Daying
authors.initials: D
authors.lastname: Wu
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.forename: Wenzhu
authors.initials: W
authors.lastname: Li
authors.forename: Jue
authors.initials: J
authors.lastname: Wang
authors.forename: Guanhua
authors.initials: G
authors.lastname: Su
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Liao
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Wang
authors.forename: Kun
authors.initials: K
authors.lastname: Liu
mesh_terms: D000208:Acute Disease; D000818:Animals; D003328:Coronary Thrombosis / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D005340:Fibrinogen / Q000096:biosynthesis*; D005786:Gene Expression Regulation; D006439:Hemodynamics; D007963:Leukocytes, Mononuclear / Q000378:metabolism* / Q000473:pathology; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D054318:No-Reflow Phenomenon / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D005340:Fibrinogen