title: Description and molecular modeling of a novel human leukocyte antigen allele: A*32:22.
abstract: We describe here the sequence and the molecular modeling of a new variant of HLA-A*32 allele officially named A*32:22. This novel allele has been detected in an Italian cord blood sample by sequence-based typing (SBT). The mutation (CAT →CGT), which has occurred at codon 151, at nucleotide position 524, implies an amino acidic change from Histidine to Arginine. Residue 151 is located on top of the molecule inside the region contacted by T cell receptor (TCR) and it is possibly involved in docking TCR. A positively charged residue is maintained on this position determining a slight change of electrostatic potential on the molecular surface. This suggests a limited functional relevance of the amino acid substitution encoded by A*32:22.
authors.affiliation: Organ and Tissue Transplantation Immunology, Regenerative Medicine Department, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy.
authors.forename: Martina
authors.initials: M
authors.lastname: Mantovani
authors.forename: Pietro
authors.initials: P
authors.lastname: Crivello
authors.forename: Sara
authors.initials: S
authors.lastname: Frison
authors.forename: Elena
authors.initials: E
authors.lastname: Longhi
authors.forename: Katharina
authors.initials: K
authors.lastname: Fleischhauer
authors.forename: Mario
authors.initials: M
authors.lastname: Scalamogna
authors.forename: Francesca
authors.initials: F
authors.lastname: Poli
mesh_terms: D000483:Alleles; D019943:Amino Acid Substitution; D001483:Base Sequence; D005312:Fetal Blood / Q000276:immunology; D005333:Fetus; D015234:HLA-A Antigens / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D055672:Static Electricity; D040681:Structural Homology, Protein
chemical_list: D015234:HLA-A Antigens