title: Complementary strand microRNAs mediate acquisition of metastatic potential in colonic adenocarcinoma.
abstract: BACKGROUND
  Altered expression of specific microRNAs (miRNA) is known to occur during colorectal carcinogenesis. However, little is known about the genome-wide alterations in miRNA expression during the neoplastic progression of primary colorectal cancers.
  
  
  METHODS
  Using a miRNA array platform, we evaluated the expression of 668 miRNA in primary colonic adenocarcinomas. Biological functions of selected miRNA were evaluated with in vitro invasion assays.
  
  
  RESULTS
  RNA was extracted for miRNA analysis from 65 primary colon cancers. We identified a seven-miRNA expression signature that differentiated stage I and stage IV primary colon cancers. We then demonstrated this signature was able to discriminate between stage II and III primary colon cancers. Six differentially expressed miRNA were downregulated in association with the development of metastases, and all 7 miRNA were complementary strand miRNA. We transfected HCT-116, a highly invasive colon cancer cell line, with corresponding downregulated miRNA and demonstrated that overexpression of three miRNA (miR200c*, miR143*, and miR424*) significantly abrogated invasive potential.
  
  
  CONCLUSIONS
  We have identified a seven-miRNA signature that is associated with metastatic potential in the primary tumor. Forced overexpression of three downregulated miRNA resulted in attenuation of in vitro invasion, suggesting direct tumor suppressive function and further supporting the biological importance of complementary strand miRNA.
authors.affiliation: Department of Biomedical Informatics, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL, USA.
authors.forename: Dung-Tsa
authors.initials: DT
authors.lastname: Chen
authors.forename: Jonathan M
authors.initials: JM
authors.lastname: Hernandez
authors.forename: David
authors.initials: D
authors.lastname: Shibata
authors.forename: Susan M
authors.initials: SM
authors.lastname: McCarthy
authors.forename: Leigh Ann
authors.initials: LA
authors.lastname: Humphries
authors.forename: Whalen
authors.initials: W
authors.lastname: Clark
authors.forename: Abul
authors.initials: A
authors.lastname: Elahi
authors.forename: Mike
authors.initials: M
authors.lastname: Gruidl
authors.forename: Domenico
authors.initials: D
authors.lastname: Coppola
authors.forename: Timothy
authors.initials: T
authors.lastname: Yeatman
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D000368:Aged; D000465:Algorithms; D014408:Biomarkers, Tumor / Q000235:genetics; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D003082:Colectomy / Q000379:methods; D003110:Colonic Neoplasms / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000032:analysis*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D012494:Sampling Studies; D012680:Sensitivity and Specificity; D046509:Tissue Culture Techniques
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs