title: Spinal hernia tissue autofluorescence spectrum.
abstract: The laser intervertebral disc decompression may provide appropriate relief in properly selected patients with contained disc herniations. The present investigation aims to characterise intervertebral disc material by autofluorescence induced by laser light. Degeneration of the intervertebral disc is associated with progressive biochemical changes in disc material. Percutaneous laser disc decompression has become rather popular for the treatment of lumbar disc herniation, but there are problems in the selection of patients. For this purpose, recognition of the disc composition is necessary. We propose a new type of spectroscopic investigation. It is advantageous to the characterization of intervertebral disc material. Intervertebral disc specimens were removed during open surgery from different disc locations. Preoperative patients' MRI was evaluated using the Pfirrmann disc degeneration and Komori scale for migrating of herniated nucleus pulposus. Adjacent slices of stained disc sections were evaluated by histology/histochemistry and autofluorescence spectra. Comparison of the MRI, spectral, histological and histochemical data was performed. The MRI Komori scale correlated with the histology Boos degeneration index. In the histochemistry, collagens other than collagens I and II of the disc were distinguished with best positive correlation coefficient (0.829) and best negative one (-0.904) of proteoglycans of sequester to Boos index. A correlation of the IV Gaussian component of the hernia spectra with the Boos index was established. The Gaussian component correlation with different collagen types and proteoglycan was determined for the disc and sequester. "Autofluorescence-based diagnosis" refers to the evaluation of disc degeneration by histological and histochemical evaluation; it can provide additional data on the degeneration of an intervertebral disc.
authors.affiliation: Division of Biomedical Technology, Institute of Applied Research, Vilnius University, Saulėtekio av. 9-III, LT-10222, Vilnius, Lithuania. darius.varanius@tmi.vu.lt
authors.forename: Darius
authors.initials: D
authors.lastname: Varanius
authors.forename: Gunaras
authors.initials: G
authors.lastname: Terbetas
authors.forename: Juozas V
authors.initials: JV
authors.lastname: Vaitkus
authors.forename: Aurelija
authors.initials: A
authors.lastname: Vaitkuviene
mesh_terms: D024042:Collagen Type I / Q000032:analysis; D024043:Collagen Type II / Q000032:analysis; D005453:Fluorescence; D006801:Humans; D007403:Intervertebral Disc / Q000473:pathology*; D055959:Intervertebral Disc Degeneration / Q000473:pathology* / Q000601:surgery; D007405:Intervertebral Disc Displacement / Q000473:pathology* / Q000601:surgery; D007834:Lasers; D008279:Magnetic Resonance Imaging; D010619:Phenazines / Q000032:analysis; D011509:Proteoglycans / Q000032:analysis
chemical_list: D024042:Collagen Type I; D024043:Collagen Type II; D010619:Phenazines; D011509:Proteoglycans; C009195:safranine T