title: Differences in the effects of beta2- and beta3-adrenoceptor agonists on spontaneous contractions of human nonpregnant myometrium.
abstract: OBJECTIVE
  This study aimed to compare the relaxant properties of BRL 37344 with p2-adrenoceptors agonist ritodrine on the contractility of human nonpregnant myometrium.
  
  
  METHODS
  The activity of myometrial strips mounted in an organ bath was recorded under isometric conditions using force transducers with digital output. Contractility before and after cumulative additions of both uterorelaxants and with preincubation with beta-adrenoceptor antagonists bupranolol, propranolol, and butoxamine were studied.
  
  
  RESULTS
  Both BRL 37344 (10(-10)-10(-4) mol/L) and ritodrine (10(-10)-10(-5) mol/L) decreased the area under curve, or AUC, value (log/C50 -6.45 +/- 0.18 and -8.71 +/- 0.35, respectively), and the degree of inhibition of spontaneous contractile activity was similar (< 30%). However BRL 37344 decreased the mean frequency of contractions, whereas ritodrine decreased the mean amplitude of contractions. The inhibition of contractions by BRL 37,344 was partially antagonized by bupranolol and propranolol, but not with butoxamine. The inhibition by ritodrine was counteracted by all these antagonists.
  
  
  CONCLUSIONS
  The effects of BRL37344 and ritodrine on human nonpregnant myometrium are quantitatively similar in respect to the inhibition of spontaneous contractility yet are also distinct due to their substantially different influences on contraction parameters. Our data indicate that beta3-adrenoceptor activation is not the sole effect of BRL 37,344 on this tissue.
authors.affiliation: Department of Biophysics, Medical University of Bialystok, Bialystok, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Pedzińska-Betiuk
authors.forename: Beata
authors.initials: B
authors.lastname: Modzelewska
authors.forename: Marcin
authors.initials: M
authors.lastname: Jóźwik
authors.forename: Maciej
authors.initials: M
authors.lastname: Jóźwik
authors.forename: Anna
authors.initials: A
authors.lastname: Kostrzewska
mesh_terms: D058666:Adrenergic beta-2 Receptor Agonists / Q000494:pharmacology*; D058667:Adrenergic beta-3 Receptor Agonists / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004983:Ethanolamines / Q000494:pharmacology*; D005260:Female; D006801:Humans; D008875:Middle Aged; D009215:Myometrium / Q000187:drug effects* / Q000502:physiology; D012312:Ritodrine / Q000494:pharmacology*; D014469:Umbilical Arteries / Q000187:drug effects; D014590:Uterine Contraction / Q000187:drug effects*
chemical_list: D058666:Adrenergic beta-2 Receptor Agonists; D058667:Adrenergic beta-3 Receptor Agonists; D004983:Ethanolamines; C057368:BRL 37344; D012312:Ritodrine