title: [Determination of 5-hydroxytryptamine in plasma by nanofiber solid phase extraction-high performance liquid chromatography].
abstract: A novel packed-nanofiber solid phase extraction coupled with high performance liquid chromatography-electrochemical detection (HPLC-ECD) method was established for the determination of 5-hydroxytryptamine (5-HT) in plasma. A 10% (v/v) HClO4 solution was used to precipitate the protein in plasma samples. After homogenizing for 1 min and centrifuging for 10 min at 12 000 r/min, the supernatant was adjusted to pH 8.5 with 0.1 mol/L sodium tetraphenylborate and then derivatized with o-phthalaldehyde (OPA) solution at 30 degrees C for 4 min. The solution was then purified and preconcentrated by the packed-nanofiber solid phase extraction column, in which methanol was used as the eluent. The analyte was analyzed by HPLC-ECD. The chromatographic separation was achieved on a Shimadzu C18 column with pH 5. 4 0. 05 mol/L phosphate buffer solution (containing 0.25 mmol/L ethylene diamine tetraacetic acid)-methanol (60:40, v/v) as mobile phase. The linear range was 5 -500 microg/L with the correlation coefficient (r2) of 0.9996. The limit of detection (S/N = 3) was 1 microg/L. And the spiked recoveries were in the range of 95.6% - 101.4% with the relative standard deviations (RSDs) of intra-day and inter-day assays below 5% (n = 3). On the basis of the advantages of simplicity, high sensitivity and good reproducibility, this method can be used for the determination of 5-HT in human plasma.
authors.affiliation: Key Laboratory of Child Development and Learning Science (Ministry of Education), Southeast University, Nanjing 210096, China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Zhou
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Liqin
authors.initials: L
authors.lastname: Chen
authors.forename: Xuejun
authors.initials: X
authors.lastname: Kang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods; D006801:Humans; D057139:Nanofibers; D012680:Sensitivity and Specificity; D012701:Serotonin / Q000097:blood*; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D012701:Serotonin