title: Chromosomal mutations responsible for fluoroquinolone resistance in Ureaplasma species in the United States.
abstract: We sequenced the full lengths of the gyrA, gyrB, parC, and parE genes in 13 fluoroquinolone-resistant Ureaplasma isolates (levofloxacin MICs, 4 to 32 μg/ml) and 10 susceptible isolates (MICs ≤ 2 μg/ml). Mutations were detected in all resistant isolates but in none of the susceptible isolates. The most prevalent mutation was the S83L substitution in the ParC protein. No plasmid-mediated fluoroquinolone resistance genes were detected.
authors.affiliation: Departments of Medicine, University of Alabama at Birmingham, Birmingham, Alabama, USA.
authors.forename: Li
authors.initials: L
authors.lastname: Xiao
authors.forename: Donna M
authors.initials: DM
authors.lastname: Crabb
authors.forename: Lynn B
authors.initials: LB
authors.lastname: Duffy
authors.forename: Vanya
authors.initials: V
authors.lastname: Paralanov
authors.forename: John I
authors.initials: JI
authors.lastname: Glass
authors.forename: Ken B
authors.initials: KB
authors.lastname: Waites
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002876:Chromosomes, Bacterial / Q000235:genetics*; D027081:DNA Gyrase / Q000235:genetics; D027101:DNA Topoisomerase IV / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics; D006801:Humans; D007527:Isoenzymes / Q000235:genetics; D064704:Levofloxacin; D008137:Longitudinal Studies; D008826:Microbial Sensitivity Tests; D009154:Mutation; D015242:Ofloxacin / Q000494:pharmacology*; D017422:Sequence Analysis, DNA; D014481:United States; D014509:Ureaplasma / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D016869:Ureaplasma Infections / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents; D007527:Isoenzymes; D064704:Levofloxacin; D015242:Ofloxacin; D027101:DNA Topoisomerase IV; D027081:DNA Gyrase