title: Infrequent finding of metallo-β-lactamase VIM-2 in carbapenem-resistant Pseudomonas aeruginosa strains from Croatia.
abstract: One hundred sixty-nine nonreplicate imipenem-resistant Pseudomonas aeruginosa strains isolated in a large hospital on the coastal region of Croatia were studied. The most active antibiotics were colistin and amikacin. Most of the isolates were multiresistant. The most prevalent serotype was O12, followed by O11. Six strains carried the bla(VIM-2) gene located in a novel class 1 integron composed in its variable part of the bla(VIM-2)-bla(oxa-10)-ΔqacF-aacA4 genes. Metallo-β-lactamase-producing strains belonged to sequence types ST235 and ST111.
authors.affiliation: Clinical Institute for Microbiology, Split University Hospital, Split, Croatia. ssardeli@kbsplit.hr
authors.forename: Sanda
authors.initials: S
authors.lastname: Sardelic
authors.forename: Branka
authors.initials: B
authors.lastname: Bedenic
authors.forename: Céline
authors.initials: C
authors.lastname: Colinon-Dupuich
authors.forename: Stjepan
authors.initials: S
authors.lastname: Orhanovic
authors.forename: Zrinka
authors.initials: Z
authors.lastname: Bosnjak
authors.forename: Vanda
authors.initials: V
authors.lastname: Plecko
authors.forename: Benoit
authors.initials: B
authors.lastname: Cournoyer
authors.forename: Gian Maria
authors.initials: GM
authors.lastname: Rossolini
mesh_terms: D000583:Amikacin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001483:Base Sequence; D003091:Colistin / Q000494:pharmacology; D017523:Croatia; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D015378:Imipenem / Q000494:pharmacology*; D032023:Integrons; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D011552:Pseudomonas Infections / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D012703:Serotyping; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D015378:Imipenem; D000583:Amikacin; C425108:beta-lactamase bla(vim-2); D001618:beta-Lactamases; D003091:Colistin