title: Estrogen receptor β transcript variants associate with oncogene expression             in endometrial cancer.
abstract: The human ESR2 gene codes for estrogen receptor β1 and for multiple splice             variants, which are suggested to exert distinct functions in the cellular estrogen             response. Given that the function of ERβ in endometrial cancer remains unclear,             we examined the expression of ERβ1, ERβ2 and various further ERβ transcript variants             and their association with selected cancer-related genes in 74 human endometrium             samples and endometrial cancer specimens by means of RT-qPCR. Additionally, we             knocked down ERβ expression in HEC-1A endometrial adenocarcinoma cells by means             of siRNA transfection. Expression of four ERβ transcript variants was significantly             elevated in cancer tissue or in G3 tumors compared to postmenopausal endometrium.             Expression of ERβ1, ERβ2, ERβ5 and five further variants was associated with the             oncogenes MYBL2 or HER2 in endometrial cancer. In addition, siRNA-triggered knockdown             of ERβ expression led to a significant decline of MYBL2 mRNA and protein levels             in endometrial cancer cells. Our observation of increased ERβ transcript levels             in cancer tissue and particularly their correlation with the expression of oncogenes,             as well as the results of our knockdown studies, suggest a role of ERβ in endometrial             carcinogenesis.
authors.affiliation: Department of Obstetrics and Gynecology, University Medical Center                     Regensburg, Regensburg, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Häring
authors.forename: Maciej
authors.initials: M
authors.lastname: Skrzypczak
authors.forename: Anette
authors.initials: A
authors.lastname: Stegerer
authors.forename: Claus
authors.initials: C
authors.lastname: Lattrich
authors.forename: Florian
authors.initials: F
authors.lastname: Weber
authors.forename: Regina
authors.initials: R
authors.lastname: Görse
authors.forename: Olaf
authors.initials: O
authors.lastname: Ortmann
authors.forename: Oliver
authors.initials: O
authors.lastname: Treeck
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics; D000368:Aged; D000369:Aged, 80 and over; D017398:Alternative Splicing / Q000235:genetics*; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D016889:Endometrial Neoplasms / Q000235:genetics*; D004717:Endometrium / Q000378:metabolism / Q000473:pathology; D047628:Estrogen Receptor alpha / Q000235:genetics* / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D008875:Middle Aged; D060787:Neoplasm Grading; D009857:Oncogenes / Q000235:genetics*; D017698:Postmenopause; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism
chemical_list: D018797:Cell Cycle Proteins; C506487:ESR1 protein, human; D047628:Estrogen Receptor alpha; C064352:MYBL2 protein, human; D012333:RNA, Messenger; D015534:Trans-Activators