title: Attenuation of vaccinia Tian Tan strain by removal of viral TC7L-TK2L and TA35R genes.
abstract: Vaccinia Tian Tan (VTT) was attenuated by deletion of the TC7L-TK2L and TA35R genes to generate MVTT3. The mutant was generated by replacing the open reading frames by a gene encoding enhanced green fluorescent protein (EGFP) flanked by loxP sites. Viruses expressing EGFP were then screened for and purified by serial plaque formation. In a second step the marker EGFP gene was removed by transfecting cells with a plasmid encoding cre recombinase and selecting for viruses that had lost the EGFP phenotype. The MVTT3 mutant was shown to be avirulent and immunogenic. These results support the conclusion that TC7L-TK2L and TA35R deletion mutants can be used as safe viral vectors or as platform for vaccines.
authors.affiliation: College of Animal Science and Veterinary Medicine, Jilin University, Jilin, People's Republic of China.
authors.forename: Shifu
authors.initials: S
authors.lastname: Kan
authors.forename: Yuhang
authors.initials: Y
authors.lastname: Wang
authors.forename: Lili
authors.initials: L
authors.lastname: Sun
authors.forename: Peng
authors.initials: P
authors.lastname: Jia
authors.forename: Yanxin
authors.initials: Y
authors.lastname: Qi
authors.forename: Jiaqiang
authors.initials: J
authors.lastname: Su
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Guohua
authors.initials: G
authors.lastname: Yang
authors.forename: Liming
authors.initials: L
authors.lastname: Liu
authors.forename: Zhuoyue
authors.initials: Z
authors.lastname: Wang
authors.forename: Jinhui
authors.initials: J
authors.lastname: Wang
authors.forename: Guangchen
authors.initials: G
authors.lastname: Liu
authors.forename: Ningyi
authors.initials: N
authors.lastname: Jin
authors.forename: Xiao
authors.initials: X
authors.lastname: Li
authors.forename: Zhuang
authors.initials: Z
authors.lastname: Ding
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation; D005260:Female; D017353:Gene Deletion; D005814:Genes, Viral / Q000235:genetics*; D042822:Genomic Instability; D006801:Humans; D007109:Immunity; D007114:Immunization; D007271:Injections, Intradermal; D051379:Mice; D008807:Mice, Inbred BALB C; D050505:Mutant Proteins / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011817:Rabbits; D014615:Vaccinia / Q000276:immunology / Q000517:prevention & control / Q000821:virology; D014616:Vaccinia virus / Q000235:genetics* / Q000276:immunology* / Q000472:pathogenicity; D019562:Viral Load; D014774:Virulence
chemical_list: D050505:Mutant Proteins