title: [Establishment of a porcine model of biliary leakage with laparoscopy].
abstract: OBJECTIVE
  To establish a animal model of biliary leakage in pigs with laparoscopy.
  
  
  METHODS
  Eight healthy Bama minipigs were subject to laparoscopic surgery under general anesthesia.The cystic duct was resected at 0.5-1.0 cm from the root and the stump was left open with the gallbladder removed. Blood routine and hepatic functions of the pigs were tested before and 24 h after the surgery, and endoscopic retrograde cholangiography (ERC) was performed 24 h after the surgery. At 48 h after the surgery, the pigs were sacrificed for observation of the stump of the cystic duct.
  
  
  RESULTS
  The gallbladder, cystic duct, cystic artery, and the anatomical relations between the gallbladder and liver and between the cystic duct and common bile duct were fully exposed under laparoscopy. White blood cells, neutrophils and direct bilirubin increased significantly after the operation (P<0.05). The cystic ducts were resected at 0.5-1 cm away from the roots of the cystic ducts in 7 pigs and at 2 cm in 1 pig.
  
  
  CONCLUSIONS
  Laparoscopy is safe and feasible for establishing the porcine model of biliary leakage.
authors.affiliation: Department of Gastroenterology and Hepatology, General Hospital of PLA, Beijing, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.forename: Enqiang
authors.initials: E
authors.lastname: Linghu
authors.forename: Daqing
authors.initials: D
authors.lastname: Jin
authors.forename: Qiuying
authors.initials: Q
authors.lastname: Ren
authors.forename: Yongchao
authors.initials: Y
authors.lastname: Zhang
authors.forename: Qingyue
authors.initials: Q
authors.lastname: Sun
mesh_terms: D000818:Animals; D001658:Biliary Fistula; D003549:Cystic Duct / Q000601:surgery; D004195:Disease Models, Animal; D005260:Female; D010535:Laparoscopy; D008297:Male; D013552:Swine; D013556:Swine, Miniature