title: Brain microdialysis in freely moving animals.
abstract: Brain microdialysis is an analytical technique used for the dynamic monitoring of brain neurochemistry in awake, freely moving animals. This technique requires the insertion of a small dialysis catheter, called a microdialysis probe, into a specific brain region, and its perfusion with an artificial extracellular fluid. The microdialysate samples, obtained from the probe outlet, can be analysed using high-performance liquid chromatography with electrochemical detection for the quantification of oxidizable molecules recovered from the extracellular space. In this chapter, we describe a protocol for performing a microdialysis setup and experiment in freely moving rats and mice. Furthermore, the high-performance liquid chromatographic determination of ascorbic acid, uric acid, catecholamines, indolamines and derivatives is described in detail.
authors.affiliation: Department of Neuroscience, Medical School, University of Sassari, Sassari, Italy.
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Bazzu
authors.forename: Alice
authors.initials: A
authors.lastname: Biosa
authors.forename: Donatella
authors.initials: D
authors.lastname: Farina
authors.forename: Ylenia
authors.initials: Y
authors.lastname: Spissu
authors.forename: Giammario
authors.initials: G
authors.lastname: Calia
authors.forename: Sonia
authors.initials: S
authors.lastname: Dedola
authors.forename: Gaia
authors.initials: G
authors.lastname: Rocchitta
authors.forename: Rossana
authors.initials: R
authors.lastname: Migheli
authors.forename: Pier Andrea
authors.initials: PA
authors.lastname: Serra
authors.forename: Maria Speranza
authors.initials: MS
authors.lastname: Desole
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000032:analysis; D001923:Brain Chemistry; D002395:Catecholamines / Q000032:analysis; D002623:Chemistry Techniques, Analytical; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004563:Electrochemistry; D045604:Extracellular Fluid / Q000737:chemistry*; D007211:Indoles / Q000032:analysis; D051379:Mice; D017551:Microdialysis / Q000379:methods*; D051381:Rats; D014527:Uric Acid / Q000032:analysis
chemical_list: D002395:Catecholamines; D007211:Indoles; D014527:Uric Acid; C067042:indolamine; D001205:Ascorbic Acid