title: Screening of natural medicines that efficiently activate neurite outgrowth in PC12 cells in C2C12-cultured medium.
abstract: We have studied the effects of natural medicines on neurite outgrowth in PC12D cells in a cultured medium of C2C12 cells. Derived from mouse myoblasts, the C2C12 cells secrete neurotrophic factors including nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3). The secretion of these neurotrophins from C2C12 cells stimulate neurite outgrowth in PC12D cells. We have screened a total of 120 samples and found five natural medicines: Trichosanthes Root, Asiasarum Root, Lycium Bark, Sinomenium Stem, and Dictamni radicis Cortex, that enhance the activity of C2C12-cultured medium to stimulate neurite outgrowth in PC12D cells. These natural medicines promoted not only neurite outgrowth but also stabilized the neurite formation in PC12D cells for several days. RT-PCR analysis showed that NGF was significantly increased with Trichosanthes and Lycium Bark. However, BDNF was slightly decreased with Lycium Bark, Sinomenium Stem, and Dictamni radicis Cortex. NT-3 was increased slightly by all of these natural medicines except Sinomenium Stem. All these five natural medicines significantly increased the number and length of neurites in PC12D cells in co-culture with C2C12 cells.
authors.affiliation: Department of Biochemistry, Hamamatsu University School of Medicine, 1-20-1 Handa-yama, Hamamatsu 431-3192, Japan. tu4nasa@hama-med.ac.jp
authors.forename: Tadayoshi
authors.initials: T
authors.lastname: Uezato
authors.forename: Eiji
authors.initials: E
authors.lastname: Sato
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Miura
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism; D002478:Cells, Cultured; D018920:Coculture Techniques; D017077:Culture Media, Conditioned / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D051379:Mice; D020932:Nerve Growth Factor / Q000378:metabolism; D009414:Nerve Growth Factors / Q000378:metabolism; D016501:Neurites / Q000187:drug effects* / Q000378:metabolism*; D020933:Neurotrophin 3 / Q000378:metabolism; D016716:PC12 Cells; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D017077:Culture Media, Conditioned; D004365:Drugs, Chinese Herbal; D009414:Nerve Growth Factors; D020933:Neurotrophin 3; D020932:Nerve Growth Factor