title: [Study on HPLC fingerprint chromatograms of Arisaematis Rhizoma].
abstract: The fingerprint chromatograms of Arisaematis Rhizoma were established by HPLC. The analysis was performed on a Lichrospher C18 (4.6 mm x 200 mm, 5 microm) column with acetonitrile-water (containing 0.1% acetic acid) as mobile phase at a flow rate of 1.0 mL x min(-1). The detection wavelength was set at 270 nm, and the column temperature was 30 degrees C. The similarities of the fingerprint chromatograms were calculated over 0.9 between 11 batches of Arisaematis Rhizoma samples by analyzing 14 common peaks with adenosine as reference substance. However, their fingerprint chromatograms were significantly different from those of Pinellia pedatisecta and P. ternate. Adenine, hypoxanthine, xanthine, uridine, guanosine, adenosine, schaftoside, and isoschaftoside were identified by comparing the retention times and their ultraviolet spectra. The method is repeatable, exclusive and can be used for identification and evaluation of Arisaematis Rhizoma.
authors.affiliation: Nanjing University of Chinese Medicine, Nanjing 210046, China. luofen0227@163.com
authors.forename: Fen
authors.initials: F
authors.lastname: Luo
authors.forename: Dan
authors.initials: D
authors.lastname: Lu
authors.forename: Yumei
authors.initials: Y
authors.lastname: Chi
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.forename: Hongli
authors.initials: H
authors.lastname: Yu
mesh_terms: D031057:Arisaema / Q000737:chemistry*; D002845:Chromatography; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000592:standards; D011786:Quality Control; D015203:Reproducibility of Results
chemical_list: D004365:Drugs, Chinese Herbal