title: [Role of centromere protein H in human gastric cancer cell proliferation].
abstract: OBJECTIVE
  To explore the role of centromere protein H (CENP-H) in the proliferation of human gastric cancer cells.
  
  
  METHODS
  RT-PCR and Western blot analysis were employed to examine the mRNA and protein expressions of CENP-H in 7 human gastric cancer cell lines and immortalized human gastric epithelial cells (GES-1). The cells were infected with the retrovirus vectors pMSCV-CENP-H or CENP-H-RNAi to establish stable cell lines with high CENP-H expression or CENP-H expression interference. MTT assay and colony formation assay were used to examine the changes in the cell proliferation after the infection.
  
  
  RESULTS
  CENP-H was over-expressed in gastric cancer cell lines AGS, BGC823, SGC-7901, MKN45, HGC27, MGC-803 and MKN28 at both mRNA and protein levels. The established AGS/CENP-H cell line with increased CENP-H expression showed enhanced proliferative activity, while the cell line MGC-803/CENP-H-RNAi with CENP-H expression interference showed an obviously lowered proliferation ability.
  
  
  CONCLUSIONS
  CENP-H promotes the proliferation of human gastric cancer cells, suggesting its important role in the occurrence and development of gastric cancer.
authors.affiliation: Department of Oncology, Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China. zaofutu@126.com
authors.forename: Tianyi
authors.initials: T
authors.lastname: Quan
authors.forename: Benfu
authors.initials: B
authors.lastname: He
authors.forename: Tiejian
authors.initials: T
authors.lastname: Liu
authors.forename: Weiming
authors.initials: W
authors.lastname: Li
authors.forename: Shangbiao
authors.initials: S
authors.lastname: Wu
authors.forename: Qingping
authors.initials: Q
authors.lastname: Jiang
authors.forename: Weiqiang
authors.initials: W
authors.lastname: Liu
authors.forename: Haibo
authors.initials: H
authors.lastname: Liu
authors.forename: Xuehu
authors.initials: X
authors.lastname: Xu
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology*
chemical_list: C121933:CENPH protein, human; D002868:Chromosomal Proteins, Non-Histone; D012333:RNA, Messenger