title: Development of a sensitive bioanalytical method for the quantification of lacosamide in rat plasma. Application to preclinical pharmacokinetics studies in rats.
abstract: A sensitive and selective high performance liquid chromatographic (HPLC) method was developed and validated for quantification of lacosamide in rat plasma. A liquid-liquid extraction procedure was optimized to extract lacosamide from rat plasma. Chromatographic separation was accomplished using a reversed phase C18 Hichrom (250×4.6 mm, 5 µm) column with the mobile phase consisting of acetonitrile-phosphate buffer (pH 3.2±0.1; 20 mM) (21:79, v/v) at a flow rate of 1 mL/min. Both intra- and inter day assay precision and accuracy were lower than 15% CV. The lower limit of quantitation was 25 ng/mL for lacosamide and the response was linear in a concentration range from 25 to 10 000 ng/mL. The developed method was successfully used for the preclinical pharmacokinetic study of lacosamide in rats.
authors.affiliation: Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, India.
authors.forename: S
authors.initials: S
authors.lastname: Shah
authors.forename: S G
authors.initials: SG
authors.lastname: Vasantharaju
authors.forename: K
authors.initials: K
authors.lastname: Arumugam
authors.forename: B S
authors.initials: BS
authors.lastname: Muddukrishna
authors.forename: N
authors.initials: N
authors.lastname: Desai
mesh_terms: D000081:Acetamides / Q000097:blood* / Q000493:pharmacokinetics; D000818:Animals; D000927:Anticonvulsants / Q000097:blood* / Q000493:pharmacokinetics; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D000078334:Lacosamide; D059625:Liquid-Liquid Extraction; D051381:Rats; D017208:Rats, Wistar; D012015:Reference Standards; D015203:Reproducibility of Results; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000081:Acetamides; D000927:Anticonvulsants; D007202:Indicators and Reagents; D012997:Solvents; D000078334:Lacosamide