title: [Optimization of supercritical fluid extraction of ursolic acid from follum eriobotryae by response surface methodology].
abstract: OBJECTIVE
  To optimize the conditions for extracting ursolic acid from Follum eriobotryae with supercritical fluid extration (SFE).
  
  
  METHODS
  The contents of ursolic acid in the extracts were determined by HPLC. Based on single factor experiment and response surface methodology, a mathematical model for SFE of ursolic acid was built.
  
  
  RESULTS
  The result showed that the optimum condition paremeters were as follows: temperature 61.6 degrees C, extraction pressure 25.8 MPa, dynamic extraction time 40 min. Under these conditions, theoretical extraction rate of ursolic acid was 3.96 mg/g.
  
  
  CONCLUSIONS
  The eptimal process is reliable.
authors.affiliation: College of Biological and Environmental Science, Zhejiang Wanli University, Ningbo 315000, China. zhanghuien@zwu.edu.cn
authors.forename: Hui-En
authors.initials: HE
authors.lastname: Zhang
authors.forename: Fei-Qiang
authors.initials: FQ
authors.lastname: Zhang
authors.forename: Cai-Xia
authors.initials: CX
authors.lastname: Wang
authors.forename: Qing-Mei
authors.initials: QM
authors.lastname: Liu
mesh_terms: D000704:Analysis of Variance; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification; D002245:Carbon Dioxide; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D018515:Plant Leaves / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D011312:Pressure; D027824:Rosaceae / Q000737:chemistry*; D013696:Temperature; D013997:Time Factors; D014315:Triterpenes / Q000032:analysis / Q000302:isolation & purification*; D000097245:Ursolic Acid
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D002245:Carbon Dioxide; D014315:Triterpenes; D000097245:Ursolic Acid