title: A novel role of brain-type ACS4 isotype in neuronal differentiation.
abstract: Acyl-CoA synthetase (ACS4) is an arachidonate preferring acyl-CoA synthetase and has been shown to play an important role in lipid metabolism. Although arachidonate and decosahexanoate play a key role in lipid metabolism and in the brain, the mechanisms of its action are still not clearly understood. In this study, we identified brain-type ACS4 mRNA by 5'-RACE. We also confirmed that the mRNA in TT2 ES cells encoded a novel ACS4 protein, which contains 41 amino acids at its N-terminus. Furthermore, we found that ACS4 proteins were up-regulated in PC-12 cell by stimulation with nerve growth factor (NGF). Importantly, knockout of ACS4 in ES cells markedly attenuated neuronal differentiation induced by NGF and all-trans retinoic acids (RA). These results indicate that the ACS4 protein specifically expressed in brain plays an important role in arachidonate metabolism and neuronal differentiation in the brain.
authors.affiliation: College of Pharmacy, The Catholic University of Korea, Jibong-ro, Wonmi-gu, Bucheon-si, Gyeonggi-do, Republic of Korea. yongyeon@catholic.ac.kr
authors.forename: Yong-Yeon
authors.initials: YY
authors.lastname: Cho
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism*; D001483:Base Sequence; D001921:Brain / Q000201:enzymology*; D003066:Coenzyme A Ligases / Q000235:genetics / Q000502:physiology*; D053595:Embryonic Stem Cells / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D055786:Gene Knockout Techniques; D051379:Mice; D008969:Molecular Sequence Data; D020932:Nerve Growth Factor / Q000494:pharmacology; D055495:Neurogenesis; D016716:PC12 Cells; D051381:Rats; D014018:Tissue Distribution
chemical_list: D016718:Arachidonic Acid; D003066:Coenzyme A Ligases; D020932:Nerve Growth Factor; C500958:Acsl4 protein, rat; C581020:Acsl4 protein, mouse