title: Marine compounds selectively induce apoptosis in female reproductive cancer cells but not in primary-derived human reproductive granulosa cells.
abstract: Anticancer properties of tyrindoleninone and 6-bromoisatin from Dicathais orbita were tested against physiologically normal primary human granulosa cells (HGC) and reproductive cancer cell lines. Tyrindoleninone reduced cancer cell viability with IC₅₀ values of 39 µM (KGN; a tumour-derived granulosa cell line), 39 μM (JAr), and 156 μM (OVCAR-3), compared to 3516 μM in HGC. Apoptosis in HGC's occurred after 4 h at 391 µM tyrindoleninone compared to 20 µM in KGN cells. Differences in apoptosis between HGC and KGN cells were confirmed by TUNEL, with 66 and 31% apoptotic nuclei at 4 h in KGN and HGC, respectively. These marine compounds therefore have potential for development as treatments for female reproductive cancers.
authors.affiliation: Department of Medical Biotechnology, School of Medicine, Flinders University, GPO Box 2100, Adelaide, S.A. 5001, Australia.
authors.forename: Vicki
authors.initials: V
authors.lastname: Edwards
authors.affiliation: School of Biological Sciences, Flinders University, GPO Box 2100, Adelaide, S.A. 5001, Australia.|School of Environmental Sciences and Management, Southern Cross University, PO Box 157, Lismore, NSW 2480, Australia.
authors.forename: Kirsten
authors.identifier: 0000-0003-4052-3888
authors.initials: K
authors.lastname: Benkendorff
authors.affiliation: Department of Medical Biotechnology, School of Medicine, Flinders University, GPO Box 2100, Adelaide, S.A. 5001, Australia.
authors.forename: Fiona
authors.initials: F
authors.lastname: Young
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D020169:Caspases / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005260:Female; D049851:Gastropoda / Q000737:chemistry*; D005833:Genital Neoplasms, Female / Q000188:drug therapy* / Q000473:pathology; D006107:Granulosa Cells / Q000187:drug effects*; D006705:Homeopathy; D006801:Humans; D006842:Hydrocarbons, Brominated / Q000494:pharmacology*; D020287:In Situ Nick-End Labeling; D007211:Indoles / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D007510:Isatin / Q000031:analogs & derivatives* / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009336:Necrosis
chemical_list: C573108:6-bromoisatin; D000970:Antineoplastic Agents; D006842:Hydrocarbons, Brominated; D007211:Indoles; C573107:tyrindoleninone; D007510:Isatin; D007770:L-Lactate Dehydrogenase; D020169:Caspases