title: Adiponectin level and gene variability are obesity and metabolic syndrome markers in a young population.
abstract: OBJECTIVE
  Human obesity is accepted as an important risk factor for development of MetS. Adiponectin is linked to central obesity and ADIPOQ variants are promising markers for understanding the genetic base of obesity-related disorders. We performed analyses of adiponectin concentrations and ADIPOQ variants and tested their associations with obesity and MetS in young subjects of Croatian origin.
  
  
  METHODS
  Biochemical and anthropometric parameters of MetS were obtained for 149 unrelated subjects. Adiponectin levels were measured by ELISA assay. ADIPOQ -11391G>A and -11377C>G were genotyped by real-time PCR.
  
  
  RESULTS
  BMI and WC, TG and GLUC showed inverse correlation, whereas HDL-C showed a positive correlation with adiponectin concentrations. For central obesity, we found association with -11377C>G and with -11391G>A polymorphisms. ADIPOQ -11377GG and -11391GA significantly increased the risk for the development of central obesity (OR 5.57 and OR 3.37, respectively). Significant association was found between -11391A, -11377G allele and haplotype and increased TG. -11377C>G and -11391G>A variant were significantly associated with the incidence of MetS. C>G mutation at position -11377 significantly increased the risk of MetS development (OR = 2.93). Compared with the -11391G homozygotes, carriers of the A allele had a significantly increased risk for the development of MetS (OR = 3.15). The test of overall association showed a statistically significant correlation of MetS with -11377C>G and -11391G>A haplotypes (p = 0.008).
  
  
  CONCLUSIONS
  Analysis of adiponectin concentration and ADIPOQ -11391G>A and -11377C>G gene variants may be clinically meaningful for estimation of MetS risk in a young population.
authors.affiliation: Department of Medical Chemistry, Biochemistry and Clinical Chemistry School of Medicine, University Hospital Centre, University of Zagreb, Zagreb, Croatia.
authors.forename: Ivana
authors.initials: I
authors.lastname: Karmelić
authors.forename: Jasna
authors.initials: J
authors.lastname: Lovrić
authors.forename: Tamara
authors.initials: T
authors.lastname: Božina
authors.forename: Hana
authors.initials: H
authors.lastname: Ljubić
authors.forename: Željka
authors.initials: Ž
authors.lastname: Vogrinc
authors.forename: Nada
authors.initials: N
authors.lastname: Božina
authors.forename: Jadranka
authors.initials: J
authors.lastname: Sertić
mesh_terms: D052242:Adiponectin / Q000097:blood* / Q000235:genetics; D000328:Adult; D017523:Croatia; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D024821:Metabolic Syndrome / Q000097:blood*; D009765:Obesity / Q000097:blood*; D020641:Polymorphism, Single Nucleotide; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D052242:Adiponectin