title: Expression of CXCL9, -10, and -11 in the aqueous humor of patients with herpetic endotheliitis.
abstract: OBJECTIVE
  To evaluate the expression of CXCL9, -10, and -11 chemokines in the aqueous humor of patients with herpetic endotheliitis.
  
  
  METHODS
  Aqueous humor was collected from 26 patients with herpetic endotheliitis and 21 control subjects. The concentrations of CXCL9, -10, and -11 in the aqueous humor were measured with enzyme-linked immunosorbent assays. The correlation between chemokine levels and clinical parameters of disease severity was analyzed. Flow cytometry was performed to count CXCR3 cells and CXCR3CD4 cells in the aqueous humor.
  
  
  RESULTS
  The concentrations of CXCL9, -10, and -11 were 429.08 ± 297.41 pg/mL, 23,102.49 ± 15,964.95 pg/mL, and 258.25 ± 103.25 pg/mL in patients with herpetic endotheliitis and 16.84 ± 16.73 pg/mL (P < 0.01), 188.45 ± 183.43 pg/mL (P < 0.01), and 7.32 ± 6.45 pg/mL (P < 0.01) in control subjects, respectively. Aqueous chemokine levels correlated significantly with keratitic precipitates and corneal edema in patients with herpetic endotheliitis. The mean percentages of CXCR3 and CXCR3CD4 cells were higher in herpetic endotheliitis patients compared with the controls.
  
  
  CONCLUSIONS
  Expression of CXCL9, -10, and -11 chemokines and their receptor CXCR3 increases in the aqueous humor of patients with herpetic endotheliitis. Chemokine levels are associated with the clinical severity of the disease.
authors.affiliation: Department of Ophthalmology and Research Institute of Medical Sciences, Chonnam National University Medical School and Hospital, Gwangju, Korea.
authors.forename: Jung-Won
authors.initials: JW
authors.lastname: Park
authors.forename: Zhengri
authors.initials: Z
authors.lastname: Li
authors.forename: Ji-Suk
authors.initials: JS
authors.lastname: Choi
authors.forename: Han-Jin
authors.initials: HJ
authors.lastname: Oh
authors.forename: Soo-Hyun
authors.initials: SH
authors.lastname: Park
authors.forename: Kyung-Chul
authors.initials: KC
authors.lastname: Yoon
mesh_terms: D001082:Aqueous Humor / Q000378:metabolism*; D002452:Cell Count; D054357:Chemokine CXCL10 / Q000378:metabolism*; D054371:Chemokine CXCL11 / Q000378:metabolism*; D054370:Chemokine CXCL9 / Q000378:metabolism*; D004728:Endothelium, Corneal / Q000378:metabolism* / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016849:Keratitis, Herpetic / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D018417:Th1 Cells / Q000378:metabolism
chemical_list: C114753:CXCL10 protein, human; C515202:CXCL11 protein, human; C080810:CXCL9 protein, human; D054357:Chemokine CXCL10; D054371:Chemokine CXCL11; D054370:Chemokine CXCL9