title: [Study on the inhibit mechanism primarily of extracts from Coptis on clinical resistant the medicine of Staphylococcus aureus].
abstract: OBJECTIVE
  To discuss the inhibit mechanism primarily of extracts from Coptis on clinical resistant the medicine of Staphylococcus aureus.
  
  
  METHODS
  Used SDS-PAGE electrophoresis, spectrophotometry and semiautomatic biochemistry analysis to detect the accumulated concentration of norfloxacin, membranin electrophoretogram and the enzymatic activity in extracellular fluid before and after Coptis extract disposition.
  
  
  RESULTS
  The accumulated concentration of medicine norfloxacin within the experiment strains which treated with Coptis extract was clear higher than that of the blank space (P < 0.01); some proteins which molecular weights were in 35 - 38 kDa rebound expressed after Coptis extract treated; The extracellular fluid enzymic activities of resistant Staphylococcus aureus had no significant difference (P > 0.05).
  
  
  CONCLUSIONS
  The inhibit mechanism primarily of extracts from Coptis on clinical resistant the medicine of Staphylococcus aureus is the results of the combined action of various chemical composition and multi-target interaction, and the exact molecular mechanism remains to be further researched.
authors.affiliation: College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China. dianxindashi513@163.com
authors.forename: Pin-Xin
authors.initials: PX
authors.lastname: Tan
authors.forename: Xiang-Xin
authors.initials: XX
authors.lastname: Liu
authors.forename: Jun-Chao
authors.initials: JC
authors.lastname: Li
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: He
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism; D000890:Anti-Infective Agents / Q000494:pharmacology*; D001219:Aspartate Aminotransferases / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D039323:Coptis / Q000737:chemistry*; D024881:Drug Resistance, Bacterial; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D008826:Microbial Sensitivity Tests; D009643:Norfloxacin / Q000493:pharmacokinetics* / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D013997:Time Factors
chemical_list: D000890:Anti-Infective Agents; D001426:Bacterial Proteins; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D009643:Norfloxacin