title: Prevalence and genotypes of Giardia duodenalis in dairy and beef cattle in farms around Charlottetown, Prince Edward Island, Canada.
abstract: Prevalence of Giardia duodenalis in dairy and beef cattle on farms around Charlottetown, Prince Edward Island (Canada) was determined by analyzing feces using direct immunofluorescence antibody microscopy. Genotypes were determined by 16S-rRNA sequencing. Fecal samples (n = 892) were collected from adult cattle in dairy tie-stall, dairy free-stall, and beef herds (10 herds each), and from calves (n = 183) from 11 dairy farms. Prevalence rates were 38% and 51% in cows and calves, respectively. Giardia duodenalis was present in all dairy herds, in 9/10 beef herds and in calves from 10/11 herds examined. Prevalence rates were 40% and 41% for cows in tie- and free-stall herds, respectively, and 27% for beef cows. Zoonotic Assemblage A was found in 12.2% of calves concomitantly infected with Assemblage E. All successfully sequenced samples (114/128) from cows corresponded to Assemblage E. Giardia duodenalis is highly prevalent in cattle herds in Prince Edward Island and Assemblage A in calves is a potential public health concern.
authors.affiliation: Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, Prince Edward Island, Canada. fuehlinger@upei.ca
authors.forename: Fabienne D
authors.initials: FD
authors.lastname: Uehlinger
authors.forename: Spencer J
authors.initials: SJ
authors.lastname: Greenwood
authors.forename: Ryan
authors.initials: R
authors.lastname: O'Handley
authors.forename: J Trenton
authors.initials: JT
authors.lastname: McClure
authors.forename: Tatjana
authors.initials: T
authors.lastname: Coklin
authors.forename: Brent R
authors.initials: BR
authors.lastname: Dixon
authors.forename: Melvin
authors.initials: M
authors.lastname: de Boer
authors.forename: Hester
authors.initials: H
authors.lastname: Zwiers
authors.forename: Herman W
authors.initials: HW
authors.lastname: Barkema
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000469:parasitology* / Q000635:transmission; D005243:Feces / Q000469:parasitology; D005260:Female; D005838:Genotype; D016829:Giardia lamblia / Q000235:genetics / Q000302:isolation & purification*; D005873:Giardiasis / Q000453:epidemiology / Q000469:parasitology / Q000635:transmission / Q000662:veterinary*; D008297:Male; D015995:Prevalence; D011325:Prince Edward Island / Q000453:epidemiology; D011634:Public Health; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D015047:Zoonoses
chemical_list: D012336:RNA, Ribosomal, 16S