title: Using imaging methods to interrogate radiation-induced cell signaling.
abstract: There is increasing emphasis on the use of systems biology approaches to define radiation-induced responses in cells and tissues. Such approaches frequently rely on global screening using various high throughput 'omics' platforms. Although these methods are ideal for obtaining an unbiased overview of cellular responses, they often cannot reflect the inherent heterogeneity of the system or provide detailed spatial information. Additionally, performing such studies with multiple sampling time points can be prohibitively expensive. Imaging provides a complementary method with high spatial and temporal resolution capable of following the dynamics of signaling processes. In this review, we utilize specific examples to illustrate how imaging approaches have furthered our understanding of radiation-induced cellular signaling. Particular emphasis is placed on protein colocalization, and oscillatory and transient signaling dynamics.
authors.affiliation: Computational Biology and Bioinformatics, Pacific Northwest National Laboratory, Richland, Washington 99352, USA.
authors.forename: Harish
authors.initials: H
authors.lastname: Shankaran
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Weber
authors.forename: Claere
authors.initials: C
authors.lastname: von Neubeck
authors.forename: Marianne B
authors.initials: MB
authors.lastname: Sowa
mesh_terms: D000818:Animals; D020013:Calcium Signaling / Q000528:radiation effects; D004249:DNA Damage; D004260:DNA Repair; D004789:Enzyme Activation / Q000528:radiation effects; D005544:Forecasting; D005786:Gene Expression Regulation / Q000528:radiation effects*; D006801:Humans; D015227:Lipid Peroxidation; D020935:MAP Kinase Signaling System / Q000528:radiation effects; D057054:Molecular Imaging / Q000379:methods*; D025941:Protein Interaction Mapping; D011494:Protein Kinases / Q000378:metabolism; D017382:Reactive Oxygen Species; D015398:Signal Transduction / Q000528:radiation effects*; D059010:Single-Cell Analysis
chemical_list: D017382:Reactive Oxygen Species; D011494:Protein Kinases