title: Acute toxicity of butachlor and atrazine to freshwater green alga Scenedesmus obliquus and cladoceran Daphnia carinata.
abstract: Both single and joint toxicity of atrazine and butachlor to freshwater green alga Scenedesmus obliquus and cladoceran Daphnia carinata isolated from South China were investigated in the present study. The 96 h-EC(50) values of atrazine and butachlor to S. obliquus were 0.0147 and 2.31 mg L(-1), while the 48 h-LC(50) values to D. carinata were 60.6 and 3.40 mg L(-1), respectively. These results suggest that atrazine could be highly toxic to S. obliquus and slightly toxic to D. carinata, while butachlor exhibits moderate toxicity to both organisms. The additive indexes of atrazine and butachlor mixtures were -2.68 (-3.02 to -2.32) to S. obliquus and 0.054 (-0.025 to 0.238) to D. carinata, respectively. Therefore, the joint action of two herbicides was significant antagonism to S. obliquus, while significant synergism was not shown to D. carinata. Moreover, significant linear correlation between the natural logarithm of herbicide concentrations and growth rates of alga S. obliquus was observed. Taken together, it is the first study reporting the toxicity endpoints for mixture of atrazine and butachlor against S. obliquus and D. carinata isolated from south China. The present results would be helpful to provide data to assess the ecological risk of both herbicides to aquatic organisms.
authors.affiliation: Key Laboratory of Ecological Agriculture of Ministry of Agriculture of the People's Republic of China, Institute of Tropical and Subtropical Ecology, South China Agricultural University, Guangzhou 510642, China. scauhhz@scau.edu.cn
authors.forename: Hongzhi
authors.initials: H
authors.lastname: He
authors.forename: Jing
authors.initials: J
authors.lastname: Yu
authors.forename: Guikui
authors.initials: G
authors.lastname: Chen
authors.forename: Wenyang
authors.initials: W
authors.lastname: Li
authors.forename: Jinbo
authors.initials: J
authors.lastname: He
authors.forename: Huashou
authors.initials: H
authors.lastname: Li
mesh_terms: D000083:Acetanilides / Q000633:toxicity*; D000818:Animals; D059001:Aquatic Organisms / Q000187:drug effects / Q000254:growth & development; D001280:Atrazine / Q000633:toxicity*; D002681:China; D003621:Daphnia; D005618:Fresh Water / Q000737:chemistry; D006540:Herbicides / Q000633:toxicity*; D044444:Scenedesmus / Q000187:drug effects* / Q000254:growth & development; D023382:Toxicity Tests, Acute; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D000083:Acetanilides; D006540:Herbicides; D014874:Water Pollutants, Chemical; C054409:butachlor; D001280:Atrazine