title: Diclofenac sodium and Imipenem action on rat intestinal mucosa: a biomechanical and histological study.
abstract: OBJECTIVE
  To study diclofenac sodium induced histological and mechanical alterations and their prevention with Imipenem in rat intestine.
  
  
  METHODS
  Male Wistar rats (n=240) were randomly assigned to four experimental groups: GI: n=60 treated with 0.9% saline IM; GII: n=60 treated with 6 mg/kg body weight diclofenac sodium IM for four days; GIII: n=60 treated with 30 mg/kg body weight Imipenem IM for four days, and GIV n=60 treated with diclofenac sodium plus Imipenem at the above doses IM for 4 days. Each group was further divided into 4 subgroups of 15 rats each and sacrificed at 4, 7, 14, and 21 days of follow-up, respectively. Abdominal cavity macroscopy and histology, and small bowel breaking strength were analyzed at each sacrifice moment.
  
  
  RESULTS
  There were no histological or mechanical alterations in normal control rats throughout the study. Ulcerated lesions in intestinal mucosa were observed and breaking strength decreased in all diclofenac sodium treated rats. Ulcerated lesions in intestinal mucosa were prevented by Imipenem in all rats.
  
  
  CONCLUSIONS
  Diclofenac sodium induced ulcerated lesions in rat intestinal mucosa can be prevented by Imipenem treatment.
authors.affiliation: Department of Surgery and Orthopedics, Gastroenterological Surgery, Botucatu Medical School, UNESP, Brazil.
authors.forename: Irio
authors.initials: I
authors.lastname: Gonçalves Junior
authors.forename: Luiz Eduardo
authors.initials: LE
authors.lastname: Naresse
authors.forename: Maria Aparecida Marchesan
authors.initials: MA
authors.lastname: Rodrigues
authors.forename: Shoiti
authors.initials: S
authors.lastname: Kobayasi
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000009:adverse effects*; D004008:Diclofenac / Q000009:adverse effects*; D015378:Imipenem / Q000494:pharmacology*; D007410:Intestinal Diseases / Q000139:chemically induced / Q000517:prevention & control*; D007413:Intestinal Mucosa / Q000187:drug effects*; D007421:Intestine, Small / Q000187:drug effects; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014456:Ulcer / Q000139:chemically induced / Q000517:prevention & control*
chemical_list: D000900:Anti-Bacterial Agents; D000894:Anti-Inflammatory Agents, Non-Steroidal; D004008:Diclofenac; D015378:Imipenem