title: Rapid reductive dechlorination of trichloroethene in contaminated ground water using biostimulation agent, BD-1, formulated from canola oil.
abstract: A case study of the bioremediation of groundwater contaminated with trichloroethene (TCE) was conducted using the biostimulation agent, BD-1. TCE levels were monitored by gas chromatography-mass spectroscopy. Total organic carbon (TOC) and volatile fatty acids (VFAs) were analyzed to investigate the environmental fate of BD-1. The effects of BD-1 on microbial activity were investigated using 16S rRNA gene-based quantitative polymerase chain reaction (qPCR) analysis. The biodegradation of BD-1 was accompanied by a reduction in TCE, and the initially high TOC levels decreased rapidly as BD-1 was transformed into VFAs. qPCR analysis showed that the genus Dehalobacter became progressively dominant through the experiment. These results suggested that BD-1 might dechlorinate TCE by activating dechlorinating bacteria.
authors.affiliation: Department of Environmental Bioscience, Meijo University. hosoda@meijo-u.ac.jp
authors.forename: Akifumi
authors.initials: A
authors.lastname: Hosoda
authors.forename: Tomonori
authors.initials: T
authors.lastname: Takahashi
authors.forename: Kousuke
authors.initials: K
authors.lastname: Numano
authors.forename: Kunihide
authors.initials: K
authors.lastname: Nakajou
authors.forename: Asumi
authors.initials: A
authors.lastname: Higashimoto
authors.forename: Minoru
authors.initials: M
authors.lastname: Toda
authors.forename: Hidehiko
authors.initials: H
authors.lastname: Arai
authors.forename: Yudai
authors.initials: Y
authors.lastname: Hotta
authors.forename: Hiroto
authors.initials: H
authors.lastname: Tamura
mesh_terms: D001419:Bacteria / Q000187:drug effects / Q000235:genetics; D001672:Biocompatible Materials / Q000494:pharmacology; D001673:Biodegradation, Environmental / Q000187:drug effects; D002245:Carbon Dioxide / Q000032:analysis; D058645:Denaturing Gradient Gel Electrophoresis; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry / Q000494:pharmacology*; D005232:Fatty Acids, Volatile / Q000032:analysis; D060587:Groundwater / Q000737:chemistry* / Q000382:microbiology; D054879:Halogenation / Q000187:drug effects; D006859:Hydrogen / Q000032:analysis; D007564:Japan; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction / Q000187:drug effects; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D000074262:Rapeseed Oil; D014241:Trichloroethylene / Q000302:isolation & purification*; D014874:Water Pollutants, Chemical / Q000302:isolation & purification*; D014876:Water Pollution / Q000032:analysis*
chemical_list: C575190:BD-1 biostimulation agent; D001672:Biocompatible Materials; D005229:Fatty Acids, Monounsaturated; D005232:Fatty Acids, Volatile; D012336:RNA, Ribosomal, 16S; D000074262:Rapeseed Oil; D014874:Water Pollutants, Chemical; D002245:Carbon Dioxide; D014241:Trichloroethylene; D006859:Hydrogen