title: [Effects of exogenous spermidine on lipid peroxidation and membrane proton pump activity of cucumber seedling leaves under high temperature stress].
abstract: Taking a relatively heat-resistant cucumber (Cucumis sativus) cultivar 'Jinchun No. 4' as test material, a sand culture experiment was conducted in growth chamber to investigate the effects of foliar spraying spermidine (Spd) on the lipid peroxidation, membrane proton pump activity, and corresponding gene expression of cucumber seedling leaves under high temperature stress. Compared with the control, foliar spraying Spd increased the plant height, stem diameter, dry and fresh mass, and leaf area significantly, and inhibited the increase of leaf relative conductivity, malondialdehyde (MDA) content, and lipoxygenase (LOX) activity effectively. Foliar spraying Spd also helped to the increase of leaf plasma membrane- and tonoplast H(+)-ATPase activity, but no significant difference was observed in the gene expression levels. These results suggested that exogenous Spd could significantly decrease the leaf lipid peroxidation and increase the proton pump activity, and thus, stabilize the leaf membrane structure and function, alleviate the damage induced by high temperature stress, and enhance the heat tolerance of cucumber seedlings.
authors.affiliation: College of Horticulture, Nanjing Agricultural University/Ministry of Agriculture Key Laboratory of Southern Vegetable Crop Genetic Improvement, Nanjing 210095, China tianjing-tianjing@163.com
authors.forename: Jing
authors.initials: J
authors.lastname: Tian
authors.forename: Shi-Rong
authors.initials: SR
authors.lastname: Guo
authors.forename: Jin
authors.initials: J
authors.lastname: Sun
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Wang
authors.forename: Yan-Juan
authors.initials: YJ
authors.lastname: Yang
authors.forename: Bin
authors.initials: B
authors.lastname: Li
mesh_terms: D018553:Cucumis sativus / Q000502:physiology*; D006358:Hot Temperature; D015227:Lipid Peroxidation / Q000187:drug effects*; D018515:Plant Leaves / Q000378:metabolism; D017494:Proton Pumps / Q000187:drug effects* / Q000378:metabolism; D036226:Seedlings / Q000502:physiology*; D013095:Spermidine / Q000494:pharmacology*; D013312:Stress, Physiological
chemical_list: D017494:Proton Pumps; D013095:Spermidine