title: Aquaporin 1 and aquaporin 4 are involved in invasion of lung cancer cells.
abstract: BACKGROUND
  An oncogenic capacity of aquaporins (AQPs) has been recently proposed. They are channel-forming membrane proteins that function as osmotically driven transepithelial and transcellular water. Most recently, overexpression of several AQPs has been reported in different types of human cancer, which indicates that AQPs may play an important role in human carcinogenesis.
  
  
  METHODS
  In this study, we were going to elucidate the involvement of aquaporin 1 and 4 (AQP1,4) in the metastasis of lung cancer.
  
  
  RESULTS
  Expression of AQP1,4 was examined by immunohistochemistry on the twenty lung cancer tissues. AQP1,4 were overexpressed in 65% (13 of 20) and 70% (14 of 20) of adenocarcinoma, while the normal lung tissues were negative. We next investigated the roles of AQP1,4 in the invasion of lung cancer cells by transwell migration assays. It is indicated that migration cells of the AQP1-shRNA or AQP4-shRNA were reduced significantly in comparison to the controls (AQP1- shRNA vs. AQP1-CTL, 5.6% vs. 15.9%, p < 0.05; AQP4- shRNA vs. AQP4-CTL, 8.9% vs. 14.8%, p < 0.05). From this study, we found AQP1 and AQP4 in lung cancer cell extravasation and spread, which may provide a functional explanation for the expression of AQP1 and AQP4 in lung cancer tissues and lung cancer cell lines.
  
  
  CONCLUSIONS
  Although further details on the molecular function of AQP1 or 4 related to tumorigenesis remain to be elucidated, our results suggest a potential role of AQP1 or 4 as novel therapeutic targets for the management of lung cancer.
authors.affiliation: Department of Respiratory, First People's Hospital of Huzhou, Zhejiang Province, China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Xie
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Wen
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Jiang
authors.forename: Huan Qin
authors.initials: HQ
authors.lastname: Fu
authors.forename: Hui
authors.initials: H
authors.lastname: Han
authors.forename: Licheng
authors.initials: L
authors.lastname: Dai
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D000328:Adult; D000368:Aged; D051398:Aquaporin 1 / Q000235:genetics / Q000378:metabolism*; D051401:Aquaporin 4 / Q000235:genetics / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism / Q000821:virology; D045744:Cell Line, Tumor; D002465:Cell Movement; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D014162:Transfection
chemical_list: C496030:AQP4 protein, human; D051401:Aquaporin 4; D014408:Biomarkers, Tumor; D051398:Aquaporin 1