title: Apoptotic cell death correlates with ROS overproduction and early cytokine expression after hypoxia-ischemia in fetal lambs.
abstract: Despite advances in neonatology, the hypoxic-ischemic injury in the perinatal period remains the single most important cause of brain injury in the newborn, leading to death or lifelong sequelae. Using a sheep model of intrauterine asphyxia, we evaluated the correlation between reactive oxygen species (ROS) overproduction, cytokine expression, and apoptotic cell death. Fetal lambs were assigned to sham group, nonasphyctic animals; and hypoxia-ischemia (HI) group, lambs subjected to 60 minutes of HI) by partial cord occlusion and sacrificed 3 hours later. Different brain regions were separated to quantify the number of apoptotic cells and the same territories were dissociated for flow cytometry studies. Our results suggest that the overproduction of ROS and the early increase in cytokine production after HI in fetal lambs correlate in a significant manner with the apoptotic index, as well as with each brain region evaluated.
authors.affiliation: Department of Cell Biology and Histology, School of Medicine and Dentistry, University of the Basque Country, Leioa, Vizcaya, Spain. daniel.alonsoa@ehu.es
authors.forename: Daniel
authors.initials: D
authors.lastname: Alonso-Alconada
authors.forename: Enrique
authors.initials: E
authors.lastname: Hilario
authors.forename: Francisco José
authors.initials: FJ
authors.lastname: Álvarez
authors.forename: Antonia
authors.initials: A
authors.lastname: Álvarez
mesh_terms: D000818:Animals; D017209:Apoptosis; D001921:Brain / Q000196:embryology / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D005311:Fetal Hypoxia / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D020925:Hypoxia-Ischemia, Brain / Q000196:embryology / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009474:Neurons / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D009928:Organ Specificity; D011897:Random Allocation; D017382:Reactive Oxygen Species / Q000378:metabolism*; D034561:Sheep, Domestic; D015854:Up-Regulation
chemical_list: D015815:Cell Adhesion Molecules; D016207:Cytokines; D009419:Nerve Tissue Proteins; D017382:Reactive Oxygen Species