title: Expression levels of microRNA-375 in colorectal carcinoma.
abstract: MicroRNAs are small, non-coding RNAs of endogenous origin. They have been increasingly shown to have altered expressions in many cancer types. The expression levels of miR-375 have not been comprehensively investigated in colorectal cancer. In this study, total RNA was extracted from 95 pairs of colorectal cancer tissues and non-tumor adjacent tissues, as well as from three colorectal cancer cell lines (HT-29, HCT-116 and SW-620). After polyadenylation and reverse transcription, we determined the expression levels of miR-375 by real-time PCR and calculated the difference in expression using the 2-∆∆Ct method. We assessed the correlation between the expression levels of miR-375 and clinicopathological characteristics of colorectal cancer. miR-375 expression was frequently downregulated in the colorectal cancer tissues compared to the non-tumor counterparts (p<0.001; paired t-test). Moreover, a significantly low expression of miR-375 was also found in the colorectal cancer cell lines (HT-29, p=0.002; HCT-116, p<0.001; SW-620, p=0.004; paired t-test). However, there were no significant correlations between the low expression of miR-375 and tumor size, histological grade, pT stage, pN stage and pTNM stage (all P>0.05, non-parametric test; Mann-Whitney U test between two groups and Kruskal-Wallis H test for three or more groups). miR-375 may be involved in the carcinogenesis of colorectal cancers and may be a potential biomarker for colorectal cancers.
authors.affiliation: Department of Surgical Oncology and General Surgery, The First Hospital of China Medical University, Shenyang, PR China.
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Dai
authors.forename: Yeunpo
authors.initials: Y
authors.lastname: Chiang
authors.forename: Zhenning
authors.initials: Z
authors.lastname: Wang
authors.forename: Yongxi
authors.initials: Y
authors.lastname: Song
authors.forename: Chong
authors.initials: C
authors.lastname: Lu
authors.forename: Peng
authors.initials: P
authors.lastname: Gao
authors.forename: Huimian
authors.initials: H
authors.lastname: Xu
mesh_terms: D014408:Biomarkers, Tumor / Q000096:biosynthesis*; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis*; D012334:RNA, Neoplasm / Q000096:biosynthesis*
chemical_list: D014408:Biomarkers, Tumor; C540933:MIRN375 microRNA, human; D035683:MicroRNAs; D012334:RNA, Neoplasm