title: Optimization of extracellular glucan production from Pleurotus eryngii and its impact on angiogenesis.
abstract: Pleurotus eryngii, an edible mushroom with therapeutic potential was optimized using response surface methodology of four-factor Box-Behnken design for maximum mycelial biomass and extracellular glucan (EPS) production. The model predicts to gain a maximal mycelial biomass and extracellular polysaccharide at 39.4 g/l; 36.04 g/l of glucose, 8.27 g/l; 7.51 g/l of yeast extract, pH 6.99; 7.07 and temperature 26.2°C; 25.84°C, respectively. The validation experiments showed that the model was significant and in close agreement with the model prediction. The evaluation of extracellular polysaccharide on angiogenesis by ex vivo CAM assay showed that there was significant inhibition in neo-vascularization.
authors.affiliation: University of Madras, Center for Advanced Studies in Botany, Chennai, India. shenbhagaraman@gmail.com
authors.forename: Ramalingam
authors.initials: R
authors.lastname: Shenbhagaraman
authors.forename: Loganathan K
authors.initials: LK
authors.lastname: Jagadish
authors.forename: Kishorekumar
authors.initials: K
authors.lastname: Premalatha
authors.forename: Venkatesan
authors.initials: V
authors.lastname: Kaviyarasan
mesh_terms: D020533:Angiogenesis Inhibitors / Q000096:biosynthesis / Q000494:pharmacology / Q000627:therapeutic use; D018533:Biomass; D049033:Chorioallantoic Membrane / Q000098:blood supply / Q000187:drug effects; D005110:Extracellular Space / Q000378:metabolism*; D005285:Fermentation; D005936:Glucans / Q000096:biosynthesis* / Q000494:pharmacology* / Q000627:therapeutic use; D015233:Models, Statistical; D025282:Mycelium / Q000254:growth & development; D009389:Neovascularization, Pathologic / Q000188:drug therapy*; D020076:Pleurotus / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism*
chemical_list: D020533:Angiogenesis Inhibitors; D005936:Glucans