title: Modulation of the activity of a polycomb-group response element in Drosophila by a mutation in the transcriptional activator woc.
abstract: Polycomb group response elements (PRE) are cis-regulatory elements that bind Polycomb group proteins. We are studying a 181-bp PRE from the Drosophilaengrailed gene. This PRE causes pairing-sensitive silencing of mini-white in transgenes. Here we show that the 181-bp PRE also represses mini-white expression in flies with only one copy of the transgene. To isolate mutations that alter the activity of the 181-bp PRE, we screened for dominant suppressors of PRE-mediated mini-white repression. Dominant suppressors of mini-white repression were rare; we recovered only nine mutations out of 68,274 progeny screened. Two of the nine mutations isolated are due to the same single amino acid change in the transcriptional activator Woc (without children). Reversion experiments show that these are dominant gain-of-function mutations in woc. We suggest that Woc can interfere with the activity of the PRE. Our data have implications for how Polycomb group proteins act to either partially repress or completely silence their target genes.
authors.affiliation: Program on Genomics of Differentiation,  Eunice Kennedy Shriver  National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892.
authors.forename: Amanda
authors.initials: A
authors.lastname: Noyes
authors.forename: Catherine
authors.initials: C
authors.lastname: Stefaniuk
authors.forename: Yuzhong
authors.initials: Y
authors.lastname: Cheng
authors.forename: James A
authors.initials: JA
authors.lastname: Kennison
authors.forename: Judith A
authors.initials: JA
authors.lastname: Kassis