title: Integration potential of mouse and human bone marrow-derived mesenchymal stem cells.
abstract: Mesenchymal stem cells (MSCs) are a multipotent cell population which has been described to exert renoprotective and regenerative effects in experimental models of kidney injury. Several lines of evidence indicate that MSCs also have the ability to contribute to nephrogenesis, suggesting that the cells can be employed in stem cell-based applications aimed at de novo renal tissue generation. In this study we re-evaluate the capacity of mouse and human bone marrow-derived MSCs to contribute to the development of renal tissue using a novel method of embryonic kidney culture. Although MSCs show expression of some genes involved in renal development, their contribution to nephrogenesis is very limited in comparison to other stem cell types tested. Furthermore, we found that both mouse and human MSCs have a detrimental effect on the ex vivo development of mouse embryonic kidney, this effect being mediated through a paracrine action. Stimulation with conditioned medium from a mouse renal progenitor population increases the ability of mouse MSCs to integrate into developing renal tissue and prevents the negative effects on kidney development, but does not appear to enhance their ability to undergo nephrogenesis.
authors.affiliation: Institute of Translational Medicine, The University of Liverpool, Liverpool, UK.
authors.forename: Maria
authors.initials: M
authors.lastname: Kuzma-Kuzniarska
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Rak-Raszewska
authors.forename: Simon
authors.initials: S
authors.lastname: Kenny
authors.forename: David
authors.initials: D
authors.lastname: Edgar
authors.forename: Bettina
authors.initials: B
authors.lastname: Wilm
authors.forename: Cristina
authors.initials: C
authors.lastname: Fuente Mora
authors.forename: Jamie A
authors.initials: JA
authors.lastname: Davies
authors.forename: Patricia
authors.initials: P
authors.lastname: Murray
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D047108:Embryonic Development; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D007668:Kidney / Q000166:cytology / Q000254:growth & development*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D019899:Paracrine Communication
chemical_list: D017077:Culture Media, Conditioned