title: Evidence of oxidative injury of the spinal cord in 2 horses with equine degenerative myeloencephalopathy.
abstract: The cervical spinal cords of 2 horses with equine degenerative myeloencephalopathy (EDM) were evaluated for evidence of oxidative damage to the central nervous system (CNS) using immunohistochemical staining for 3-nitrotyrosine (3-NT) and 4-hydroxynonenol (4-HNE). Neurons of the CNS from horses with EDM had positive immunohistochemical staining, whereas control samples did not, thus supporting the theory that oxidative damage is a potential underlying factor in horses with EDM. In addition, serum vitamin E concentration was low in both EDM-affected horses, and vitamin E concentration was also deficient in the cerebrospinal fluid in 1 EDM horse, further supporting the association between low vitamin E concentrations and oxidative damage to the CNS. Continued research is necessary to further define the pathophysiologic mechanisms of EDM.
authors.affiliation: Department of Veterinary Clinical Sciences, Lloyd Veterinary Medical Center, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA. dwong@iastate.edu
authors.forename: D M
authors.initials: DM
authors.lastname: Wong
authors.forename: A
authors.initials: A
authors.lastname: Ghosh
authors.forename: A J
authors.initials: AJ
authors.lastname: Fales-Williams
authors.forename: J S
authors.initials: JS
authors.lastname: Haynes
authors.forename: A G
authors.initials: AG
authors.lastname: Kanthasamy
mesh_terms: D000447:Aldehydes / Q000032:analysis; D000818:Animals; D001259:Ataxia / Q000662:veterinary; D001927:Brain Diseases / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary*; D002490:Central Nervous System / Q000473:pathology; D005260:Female; D006734:Horse Diseases / Q000175:diagnosis* / Q000473:pathology; D006736:Horses; D007150:Immunohistochemistry / Q000662:veterinary; D019636:Neurodegenerative Diseases / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary*; D018384:Oxidative Stress; D013118:Spinal Cord Diseases / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary*; D014443:Tyrosine / Q000031:analogs & derivatives / Q000032:analysis; D014810:Vitamin E / Q000134:cerebrospinal fluid; D014811:Vitamin E Deficiency / Q000662:veterinary
chemical_list: D000447:Aldehydes; D014810:Vitamin E; C002744:3-nitrotyrosine; D014443:Tyrosine; C027576:4-hydroxy-2-nonenal