title: Addressable discrete-line-scanning multiphoton microscopy based on a spatial light modulator.
abstract: We developed a novel addressable discrete-line-scanning multiphoton microscope with high lateral and axial resolutions based on a spatial light modulator. Our discrete-line-focus design eliminates the cross talk that occurs in conventional one-dimensional line-scanning multiphoton microscopies. Additionally, a phase-only spatial light modulator is able to scan only a sample's target area by generating a specific discrete line focus according to the shape and location of the target area. Compared with other multiphoton microscopies, this technique shortens scanning time and minimizes photodamage by concentrating scanning energy and dwell time on the area of interest.
authors.affiliation: Department of Bioengineering and COMSET, Clemson University, Clemson, South Carolina 29634, USA.
authors.forename: Wan
authors.initials: W
authors.lastname: Qin
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Shao
authors.forename: Honghai
authors.initials: H
authors.lastname: Liu
authors.forename: Xiang
authors.initials: X
authors.lastname: Peng
authors.forename: Hanben
authors.initials: H
authors.lastname: Niu
authors.forename: Bruce
authors.initials: B
authors.lastname: Gao
mesh_terms: D000818:Animals; D008027:Light; D008853:Microscopy / Q000379:methods*; D032383:Myocytes, Cardiac / Q000166:cytology; D017785:Photons; D051381:Rats