title: New monolithic chromatographic supports for macromolecules immobilization: challenges and opportunities.
abstract: This mini-review reports on some recent advances in the field of immobilized protein employing both silica and polymer-based monoliths as supports, and their application in affinity chromatography and immobilized enzyme reactors (IMERs) developments. The major emphasis is put on some interesting challenges and opportunities related to the development of new monolithic affinity supports based on biofriendly sol-gel inorganic monoliths with entrapped proteins and on organic monolithic supports with improved hydrophilicity for IMERs development in proteomic studies. The ease of preparation of monoliths and the multitude of functionalization techniques, make monoliths interesting for an increasing number of biochemical and medical applications.
authors.affiliation: Department of Drug Sciences, University of Pavia, Via Taramelli 12, 27100 Pavia, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Calleri
authors.forename: S
authors.initials: S
authors.lastname: Ambrosini
authors.forename: C
authors.initials: C
authors.lastname: Temporini
authors.forename: G
authors.initials: G
authors.lastname: Massolini
mesh_terms: D001671:Biochemistry / Q000379:methods; D002845:Chromatography / Q000379:methods*; D002846:Chromatography, Affinity / Q000379:methods; D004800:Enzymes, Immobilized / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D008024:Ligands; D046911:Macromolecular Substances / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods; D009930:Organic Chemicals / Q000737:chemistry; D044367:Phase Transition; D011108:Polymers / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D040901:Proteomics / Q000379:methods; D012822:Silicon Dioxide / Q000737:chemistry; D013329:Structure-Activity Relationship; D013499:Surface Properties
chemical_list: D004800:Enzymes, Immobilized; D008024:Ligands; D046911:Macromolecular Substances; D009930:Organic Chemicals; D011108:Polymers; D011506:Proteins; D012822:Silicon Dioxide