title: Bone marrow cells of swine: collection and separation.
abstract: Bone marrow is a source of stem cells for greater and easier access, which is widely studied as a provider of hematopoietic and mesenchymal cells for various purposes, mainly therapeutic by the advances in research involving cell therapy. The swine is an animal species commonly used in the pursuit of development of experimental models. Thus, this study aimed to standardize protocol for collection and separation of bone marrow in swines, since this species is widely used as experimental models for various diseases. Twelve animals were used, which underwent bone marrow puncture with access from the iliac crest and cell separation by density gradient followed by a viability test with an average of 98% of viable cells. Given our results, we can ensure the swine as an excellent model for obtaining and isolation of mononuclear cells from bone marrow, stimulating several studies addressing the field of cell therapy.
authors.affiliation: Faculty of Veterinary Medicine, Morphological Research Laboratory Animal, Federal Rural University of Amazonia, UFRA, Belém 66.077-901 Pará, Brazil. ebranco.ufra@gmail.com
authors.forename: Érika
authors.initials: É
authors.lastname: Branco
authors.forename: Rosa
authors.initials: R
authors.lastname: Cabral
authors.forename: Bruno Duarte
authors.initials: BD
authors.lastname: Gomes
authors.forename: José Roberto
authors.initials: JR
authors.lastname: Kfoury
authors.forename: Maria Angelica
authors.initials: MA
authors.lastname: Miglino
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology*; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D003584:Cytological Techniques / Q000379:methods; D013552:Swine