title: [Extraction and analysis of chemical components of essential oil in Thymus vulgaris of tissue culture].
abstract: OBJECTIVE
  To extract the essential oils from the Seedlings, the Aseptic Seedlings and the Tissue Culture Seedlings of Thymus vulgaris and analyze their chemical components and the relative contents.
  
  
  METHODS
  The essential oils were extracted by steam distillation, the chemical components and the relative contents were identified and analyzed by gas chromatography-mass spectrometry (GC/MS) and peak area normalization method.
  
  
  RESULTS
  The main chemical components of essential oil in these three samples had no significant difference, they all contained the main components of essential oil in Thymus vulgaris: Thymol, Carvacrol, o-Cymene, gamma-Terpinene, Caryophyllene et al. and only had a slight difference in the relative content.
  
  
  CONCLUSIONS
  This study provides important theoretical foundation and data reference for further study on production of essential oil in thyme by tissue culture technology.
authors.affiliation: School of Applied Biological Engineering, Shenzhen Polytechnic, Shenzhen 518055, China. lxd1819@yahoo.com.cn
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.forename: Shi-Qian
authors.initials: SQ
authors.lastname: Xu
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Li
authors.forename: Zhi-Hui
authors.initials: ZH
authors.lastname: Cheng
authors.forename: Jian-Zhang
authors.initials: JZ
authors.lastname: Dang
mesh_terms: D000081005:Cyclohexane Monoterpenes; D000081007:Cymenes; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D015394:Molecular Structure; D039821:Monoterpenes / Q000032:analysis / Q000737:chemistry; D009822:Oils, Volatile / Q000737:chemistry* / Q000302:isolation & purification; D012717:Sesquiterpenes / Q000032:analysis* / Q000737:chemistry; D013943:Thymol / Q000032:analysis* / Q000737:chemistry; D046930:Thymus Plant / Q000737:chemistry*; D046509:Tissue Culture Techniques
chemical_list: D000081005:Cyclohexane Monoterpenes; D000081007:Cymenes; D039821:Monoterpenes; D009822:Oils, Volatile; D012717:Sesquiterpenes; D013943:Thymol; C018669:gamma-terpinene; C073316:carvacrol