title: A dendrimer-based immunosensor for improved capture and detection of tumor necrosis factor-α cytokine.
abstract: A dendrimer-based sandwich type enzyme-linked immunosorbent assay (ELISA) was developed for the improved detection of recombinant human tumor necrosis factor-alpha (TNF-α) for early diagnosis of perinatal diseases. Hydroxyl-terminated generation four poly(amidoamine) dendrimer (G4-OH) was used for the development of a solid phase bio-sensing platform. The surface of the ELISA plate was modified with polyethylene-glycol (PEG) and thiol-functionalized G4-OH was immobilized on the PEG-functionalized plate. A capture antibody was oxidized and covalently immobilized onto the dendrimer-modified ELISA plate, which provides favorable orientation for the antigen binding sites toward the analyte. The dendrimer-modified plate showed enhanced sensitivity, and the detection limit for TNF-α was found to be 0.48 pg mL(-1), which is significantly better than the commercially available ELISA kit. The selectivity of the dendrimer-modified ELISA plate was further evaluated with a mixture of cytokines, which showed results for similar to that of TNF-α alone. The modified plate provides a greater opportunity for the detection of a wide range of cytokines and biomarkers.
authors.affiliation: Department of Chemical Engineering and Materials Science, Wayne State University, Detroit, MI 48202, USA.
authors.forename: Admira
authors.initials: A
authors.lastname: Bosnjakovic
authors.forename: Manoj K
authors.initials: MK
authors.lastname: Mishra
authors.forename: Hye Jung
authors.initials: HJ
authors.lastname: Han
authors.forename: Roberto
authors.initials: R
authors.lastname: Romero
authors.forename: Rangaramanujam M
authors.initials: RM
authors.lastname: Kannan
mesh_terms: D055910:Antibodies, Immobilized / Q000276:immunology; D050091:Dendrimers / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D010084:Oxidation-Reduction; D011073:Polyamines / Q000737:chemistry; D011092:Polyethylene Glycols; D011994:Recombinant Proteins / Q000032:analysis / Q000235:genetics / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis* / Q000235:genetics / Q000276:immunology
chemical_list: D055910:Antibodies, Immobilized; D050091:Dendrimers; C531249:Poly(amidoamine); D011073:Polyamines; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D011092:Polyethylene Glycols