title: Platelet-rich plasma promotes angiogenesis of prefabricated vascularized bone graft.
abstract: OBJECTIVE
  The objective was to assess the impact of platelet-rich plasma (PRP) on angiogenesis and bone formation of tissue-engineered bone in the prefabricated stage.
  
  
  METHODS
  Both thighs of New Zealand white rabbits were used as prefabricated vascularized bone grafts using a combination of bone mesenchymal stem cells and vascular bundles in a titanium cage filled with β-tricalcium phosphate ceramic. PRP was applied in the test group, and the same procedure was performed in the control group without the application of PRP. After 4, 8, and 12 weeks, delayed static bone scanning with technetium-99m methylene diphosphonate was performed before sacrifice, and the tissue-engineered bone samples were collected for immunohistochemical analysis using a monoclonal antibody against CD31 and histologic analysis.
  
  
  RESULTS
  The results showed superior angiogenesis in the PRP group compared with the control group at each time point as determined by bone scintigraphy and immunohistochemical examinations. The results of histologic analysis also showed that there was more bone formation in the PRP group than in the control group at each time point.
  
  
  CONCLUSIONS
  The application of autologous PRP was an effective strategy for increasing angiogenesis and bone formation in tissue-engineered bone and had potential significance for clinical applications.
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Stomatology, Fourth Military Medical University, Xi'an, China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Dong
authors.forename: Bei
authors.initials: B
authors.lastname: Li
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.forename: Shizhu
authors.initials: S
authors.lastname: Bai
authors.forename: Guoquan
authors.initials: G
authors.lastname: Li
authors.forename: Ao
authors.initials: A
authors.lastname: Ding
authors.forename: Jinlong
authors.initials: J
authors.lastname: Zhao
authors.forename: Yanpu
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001854:Bone Marrow Cells / Q000166:cytology; D001857:Bone Matrix / Q000098:blood supply / Q000473:pathology; D016025:Bone Transplantation / Q000000981:diagnostic imaging / Q000473:pathology*; D002130:Calcium Phosphates / Q000737:chemistry; D018929:Cell Culture Techniques; D007150:Immunohistochemistry; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods; D018919:Neovascularization, Physiologic / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000032:analysis; D053657:Platelet-Rich Plasma / Q000502:physiology*; D011817:Rabbits; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals; D013669:Technetium Tc 99m Medronate; D013848:Thigh / Q000098:blood supply / Q000000981:diagnostic imaging / Q000601:surgery; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry; D014025:Titanium / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D019275:Radiopharmaceuticals; C485817:beta-tricalcium phosphate; D014025:Titanium; D013669:Technetium Tc 99m Medronate