title: S-Nitrosoglutathione is a component of wound- and salicylic acid-induced systemic responses in Arabidopsis thaliana.
abstract: S-Nitrosoglutathione (GSNO) is a bioactive, stable, and mobile reservoir of nitric oxide (NO), and an important player in defence responses to herbivory and pathogen attack in plants. It has been demonstrated previously that GSNO reductase (GSNOR) is the main enzyme responsible for the in vivo control of intracellular levels of GSNO. In this study, the role of S-nitrosothiols, in particular of GSNO, in systemic defence responses in Arabidopsis thaliana was investigated further. It was shown that GSNO levels increased rapidly and uniformly in injured Arabidopsis leaves, whereas in systemic leaves GSNO was first detected in vascular tissues and later spread over the parenchyma, suggesting that GSNO is involved in the transmission of the wound mobile signal through the vascular tissue. Moreover, GSNO accumulation was required to activate the jasmonic acid (JA)-dependent wound responses, whereas the alternative JA-independent wound-signalling pathway did not involve GSNO. Furthermore, extending previous work on the role of GSNOR in pathogenesis, it was shown that GSNO acts synergistically with salicylic acid in systemic acquired resistance activation. In conclusion, GSNOR appears to be a key regulator of systemic defence responses, in both wounding and pathogenesis.
authors.affiliation: Departament de Bioquímica i Biologia Molecular, Facultat de Biociències, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.
authors.forename: M Carme
authors.initials: MC
authors.lastname: Espunya
authors.forename: Roberto
authors.initials: R
authors.lastname: De Michele
authors.forename: Aurelio
authors.initials: A
authors.lastname: Gómez-Cadenas
authors.forename: M Carmen
authors.initials: MC
authors.lastname: Martínez
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism; D003517:Cyclopentanes / Q000378:metabolism / Q000494:pharmacology; D060467:Disease Resistance / Q000187:drug effects / Q000276:immunology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005980:Glutathione Reductase / Q000378:metabolism; D054883:Oxylipins / Q000378:metabolism / Q000494:pharmacology; D018515:Plant Leaves / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D030821:Plants, Genetically Modified; D026422:S-Nitrosoglutathione / Q000378:metabolism*; D026403:S-Nitrosothiols / Q000378:metabolism; D020156:Salicylic Acid / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D054883:Oxylipins; D026403:S-Nitrosothiols; D026422:S-Nitrosoglutathione; C011006:jasmonic acid; D005980:Glutathione Reductase; C521253:S-nitrosoglutathione reductase, Arabidopsis; D020156:Salicylic Acid