title: In vitro activity of antimicrobial agents against Pseudomonas tolaasii, pathogen of cultivated button mushroom.
abstract: In vitro antibacterial activity tests of seven biofungicides (Ekstrasol, Bisolbisan, Bisolbifit, Serenade, Sonata, Timorex, F-Stop) and two disinfectants (colloidal silver alone and in combination with hydrogen peroxide) against the Pseudomonas tolaasii strain (NS3B6) were carried out by the disc-diffusion, broth microdilution and broth macrodilution method. Biofungicides tested in this study did not exhibit any antimicrobial activity in neither one of the methods used. Disc diffusion method revealed high sensitivity of the tested P. tolaasii strain to Ecocute based on colloidal silver and hydrogen peroxide. Both microdilution and macrodilution methods identified the same MICs and MBCs of Ecocute (0.19 mg/L) for P. tolaasii strain. MICs and MBCs values of silver alone were much higher (10 mg/L) compared to silver in combination with hydrogen peroxide.
authors.affiliation: Institute of Pesticides and Environmental Protection, Zemun-Belgrade, Serbia.
authors.forename: Biljana
authors.initials: B
authors.lastname: Todorović
authors.forename: Svetlana
authors.initials: S
authors.lastname: Milijasević-Marčić
authors.forename: Ivana
authors.initials: I
authors.lastname: Potočnik
authors.forename: Miloš
authors.initials: M
authors.lastname: Stepanović
authors.forename: Emil
authors.initials: E
authors.lastname: Rekanović
authors.forename: Ljiljana
authors.initials: L
authors.lastname: Nikolić-Bujanović
authors.forename: Milan
authors.initials: M
authors.lastname: Cekerevac
mesh_terms: D000364:Agaricus; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D001412:Bacillus subtilis; D061046:Biological Control Agents; D003102:Colloids; D052978:Disk Diffusion Antimicrobial Tests; D004338:Drug Combinations; D005659:Fungicides, Industrial / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D011549:Pseudomonas / Q000187:drug effects*; D012834:Silver / Q000494:pharmacology*; D020947:Tea Tree Oil / Q000494:pharmacology*
chemical_list: D000891:Anti-Infective Agents, Local; D061046:Biological Control Agents; D003102:Colloids; D004338:Drug Combinations; D005659:Fungicides, Industrial; D012834:Silver; D020947:Tea Tree Oil; D006861:Hydrogen Peroxide