title: Molecular epidemiology of the attachment glycoprotein (G) gene in respiratory syncytial virus in children with acute respiratory infection in Japan in 2009/2010.
abstract: This study performed a detailed genetic analysis of the glycoprotein (G) gene of respiratory syncytial virus (RSV) detected in 50 Japanese children with acute respiratory infection (ARI) in the 2009/2010 season. A phylogenetic tree constructed by the neighbour-joining method showed that 34 and 16 of the RSV strains could be classified into subgroups A and B, respectively. Strains belonging to subgroups A and B were further subdivided into GA2 and BA, respectively. The nucleotide and deduced amino acid sequence identities were relatively high among these strains (>90%). The deduced amino acid sequences implied that a relatively high frequency of amino acid substitutions occurred in the C-terminal 3rd hypervariable region of the G protein in these strains. In addition, some positively selected sites were estimated. The results suggest that RSV with genotypes GA2 and BA was associated with ARI in Japanese children in 2009/2010.
authors.affiliation: Aomori Prefectural Institute of Public Health and Environment, 1-1-1 Higashitsukurimichi, Aomori-shi, Aomori 030-8566, Japan.
authors.forename: Ayako
authors.initials: A
authors.lastname: Yoshida
authors.affiliation: Kumamoto Prefectural Institute of Public Health and Environmental Sciences, 1240-1 Kurisaki-machi, Uto-shi, Kumamoto 869-0425, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Kiyota
authors.affiliation: Gunma Prefectural Institute of Public Health and Environmental Sciences, 378 Kamioki-machi, Maebashi-shi, Gunma 371-0052, Japan.
authors.forename: Miho
authors.initials: M
authors.lastname: Kobayashi
authors.affiliation: Kumamoto Prefectural Institute of Public Health and Environmental Sciences, 1240-1 Kurisaki-machi, Uto-shi, Kumamoto 869-0425, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Nishimura
authors.affiliation: Aomori Prefectural Institute of Public Health and Environment, 1-1-1 Higashitsukurimichi, Aomori-shi, Aomori 030-8566, Japan.
authors.forename: Rika
authors.initials: R
authors.lastname: Tsutsui
authors.affiliation: Gunma Prefectural Institute of Public Health and Environmental Sciences, 378 Kamioki-machi, Maebashi-shi, Gunma 371-0052, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tsukagoshi
authors.affiliation: Fukui Prefectural Institute of Public Health and Environmental Science, 39-4 Harame-cho, Fukui-shi, Fukui 910-8551, Japan.
authors.forename: Eiko
authors.initials: E
authors.lastname: Hirano
authors.affiliation: Influenza Virus Research Center and Infectious Disease Surveillance Center, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama-shi, Tokyo 208-0011, Japan.
authors.forename: Norio
authors.initials: N
authors.lastname: Yamamoto
authors.affiliation: Department of Molecular Biodefence Research, Yokohama City University Graduate School of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama-shi, Kanagawa 236-0004, Japan.
authors.forename: Akihide
authors.initials: A
authors.lastname: Ryo
authors.affiliation: Gunma Prefectural Institute of Public Health and Environmental Sciences, 378 Kamioki-machi, Maebashi-shi, Gunma 371-0052, Japan.
authors.forename: Mika
authors.initials: M
authors.lastname: Saitoh
authors.affiliation: Kumamoto Prefectural Institute of Public Health and Environmental Sciences, 1240-1 Kurisaki-machi, Uto-shi, Kumamoto 869-0425, Japan.
authors.forename: Seiya
authors.initials: S
authors.lastname: Harada
authors.affiliation: Aomori Prefectural Institute of Public Health and Environment, 1-1-1 Higashitsukurimichi, Aomori-shi, Aomori 030-8566, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Inoue
authors.affiliation: Gunma Prefectural Institute of Public Health and Environmental Sciences, 378 Kamioki-machi, Maebashi-shi, Gunma 371-0052, Japan.
authors.forename: Kunihisa
authors.initials: K
authors.lastname: Kozawa
authors.affiliation: Department of Surgery, Institute of Medical Sciences, Kyorin University, 6-20-2 Shinkawa, Mitaka-shi, Tokyo 181-8611, Japan.
authors.forename: Ryota
authors.initials: R
authors.lastname: Tanaka
authors.affiliation: Infectious Disease Surveillance Center, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama-shi, Tokyo 208-0011, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Noda
authors.affiliation: Infectious Disease Surveillance Center, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama-shi, Tokyo 208-0011, Japan.
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Okabe
authors.affiliation: Influenza Virus Research Center and Infectious Disease Surveillance Center, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama-shi, Tokyo 208-0011, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Tashiro
authors.affiliation: Yamagata Prefectural Institute of Public Health, 1-6-6 Toka-machi, Yamagata-shi, Yamagata 990-0031, Japan.
authors.forename: Katsumi
authors.initials: K
authors.lastname: Mizuta
authors.affiliation: Infectious Disease Surveillance Center, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama-shi, Tokyo 208-0011, Japan.|Gunma Prefectural Institute of Public Health and Environmental Sciences, 378 Kamioki-machi, Maebashi-shi, Gunma 371-0052, Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Kimura
mesh_terms: D000293:Adolescent; D019943:Amino Acid Substitution; D002648:Child; D002675:Child, Preschool; D016000:Cluster Analysis; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D007564:Japan / Q000453:epidemiology; D008297:Male; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D018357:Respiratory Syncytial Virus Infections / Q000453:epidemiology* / Q000821:virology*; D018113:Respiratory Syncytial Virus, Human / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014759:Viral Envelope Proteins / Q000235:genetics*
chemical_list: D014759:Viral Envelope Proteins; C116204:attachment protein G