title: Iron starvation regulates the type III secretion system in Bordetella bronchiseptica.
abstract: The type III secretion system (T3SS) plays a key role in the exertion of full virulence by Bordetella bronchiseptica. However, little is known about the environmental stimuli that induce expression of T3SS genes. Here, it is reported that iron starvation is a signal for T3SS gene expression in B. bronchiseptica. It was found that, when B. bronchiseptica is cultured under iron-depleted conditions, secretion of type III secreted proteins is greater than that in bacteria grown under iron-replete conditions. Furthermore, it was confirmed that induction of T3SS-dependent host cell cytotoxicity and hemolytic activity is greatly enhanced by infection with iron-depleted Bordetella. In contrast, production of filamentous hemagglutinin is reduced in iron-depleted Bordetella. Thus, B. bronchiseptica controls the expression of virulence genes in response to iron starvation.
authors.affiliation: Laboratory of Bacterial Infection, Graduate School of Infection Control Sciences, Kitasato University, Tokyo 108-8641, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Kurushima
authors.forename: Asaomi
authors.initials: A
authors.lastname: Kuwae
authors.forename: Akio
authors.initials: A
authors.lastname: Abe
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D058947:Bacterial Secretion Systems; D001427:Bacterial Toxins / Q000378:metabolism; D016950:Bordetella bronchiseptica / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D004912:Erythrocytes / Q000187:drug effects; D015964:Gene Expression Regulation, Bacterial; D006461:Hemolysis; D007501:Iron / Q000378:metabolism*; D051379:Mice; D011817:Rabbits; D037521:Virulence Factors / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D058947:Bacterial Secretion Systems; D001427:Bacterial Toxins; D037521:Virulence Factors; D007501:Iron