title: Characterization of Fusarium species section Liseola by restriction analysis of the IGS region.
abstract: Fusarium species section Liseola namely F. fujikuroi, F. proliferatum, F. andiyazi, F. verticillioides, and F. sacchari are  well-known plant pathogens on rice, sugarcane and maize. In the present study,  restriction analysis of the intergenic spacer regions (IGS) was used to  characterize the five Fusarium species isolated from rice, sugarcane and  maize collected from various locations in Peninsular Malaysia. From the  analysis, and based on restriction patterns generated by the six restriction  enzymes, Bsu151, BsuRI, EcoRI, Hin6I, HinfI,  and MspI, 53 haplotypes were recorded among 74 isolates. HinfI  showed the most variable restriction patterns (with 11 patterns), while EcoRI  showed only three patterns. Although a high level of variation was observed, it  was possible to characterize closely related species and isolates from  different species. UPGMA cluster analysis showed that the isolates of Fusarium from the same species were grouped together regardless of the hosts. We  conclude that restriction analysis of the IGS regions can be used to characterize Fusarium species section Liseola and to discriminate closely related  species as well as to clarify their taxonomic position.
authors.affiliation: School of Biological Sciences, Universiti Sains Malaysia, Pulau Pinang, Malaysia.
authors.forename: M H
authors.initials: MH
authors.lastname: Heng
authors.forename: S
authors.initials: S
authors.lastname: Baharuddin
authors.forename: Z
authors.initials: Z
authors.lastname: Latiffah
mesh_terms: D004262:DNA Restriction Enzymes; D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D005670:Fusarium / Q000235:genetics* / Q000302:isolation & purification; D012275:Oryza / Q000382:microbiology; D010802:Phylogeny; D015183:Restriction Mapping / Q000379:methods*; D031786:Saccharum / Q000382:microbiology; D003313:Zea mays / Q000382:microbiology
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D004262:DNA Restriction Enzymes