title: Response of alkaline phosphatases in the cyanobacterium Anabaena sp. FACHB 709 to inorganic phosphate starvation.
abstract: Alkaline phosphatases (APases) play a crucial role in phosphorus (P) metabolism and regulation, but their physiological functions largely remain unclear in cyanobacteria. Here, we identified four putative APase genes, designated as phoA-709, phoD1-709, phoD2-709, and phoS-709, in the cyanobacterium Anabaena sp. FACHB 709, and investigated their response to inorganic phosphate (P(i)) starvation. With the exception of phoD2-709, three other APase genes were expressed at a constant and relative low level in P(i)-replete medium, whereas the expression of all four APase genes was elevated in response to P(i) starvation but phoA-709 significantly. However, disruption of phoA-709 did not affect the total APase activity but caused the expressional up-regulation of phoD1-709 and phoS-709 under P(i)-sufficient and P(i)-limiting conditions. These suggest that, the four APases of Anabaena sp. FACHB 709 are involved in P metabolism and regulation, and PhoA-709 is the main, yet dispensable, APase.
authors.affiliation: Department of Environment Engineering, School of Environment, Jiangsu University, Zhenjiang, Jiangsu 212013, China.
authors.forename: Zhaoying
authors.initials: Z
authors.lastname: Liu
authors.forename: Chundu
authors.initials: C
authors.lastname: Wu
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism*; D017033:Anabaena / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D017353:Gene Deletion; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D010710:Phosphates / Q000378:metabolism*
chemical_list: D003470:Culture Media; D010710:Phosphates; D000469:Alkaline Phosphatase