title: Production and immobilization of a novel thermoalkalophilic extracellular amylase from bacilli isolate.
abstract: A Thermoalkalophilic amylase was produced from an environmental bacterial isolate. The enzyme was then immobilized through its amino groups onto the epoxy rings of magnetic poly glycidyl methacrylate [m-poly (GMA)] beads. The free enzyme was active within a large pH range, between 7 and 12 and displayed the optimum activity at 95°C and pH 10. The immobilization appeared to increase the stability of the enzyme as its bound form showed optimum activity at 105°C and pH 11.0. Kinetic studies demonstrated that immobilized enzyme had higher K(m) and lower V(max) values. The activity of the free and bound enzyme was determined, at 37°C and pH 10.0 and pH 11.0, respectively, in the presence of various organic solvents and detergents (5%, v/v). Results obtained indicated that detergents, sodium dodecyl sulfate (SDS) and TritonX-100, caused six fold increase and that various organic solvents also increased the activity of the amylase.
authors.affiliation: Cumhuriyet University, Department of Molecular Biology and Genetics, Sivas 58140, Turkey. bakkaya@cumhuriyet.edu.tr
authors.forename: Birnur
authors.initials: B
authors.lastname: Akkaya
authors.forename: Ali Fazil
authors.initials: AF
authors.lastname: Yenidunya
authors.forename: Recep
authors.initials: R
authors.lastname: Akkaya
mesh_terms: D000681:Amylases / Q000096:biosynthesis* / Q000737:chemistry* / Q000378:metabolism; D001407:Bacillus / Q000166:cytology* / Q000201:enzymology / Q000302:isolation & purification* / Q000378:metabolism; D003902:Detergents / Q000494:pharmacology; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000096:biosynthesis* / Q000737:chemistry* / Q000378:metabolism; D005110:Extracellular Space / Q000201:enzymology*; D007700:Kinetics; D008670:Metals / Q000494:pharmacology; D008863:Microspheres; D009930:Organic Chemicals / Q000494:pharmacology; D012997:Solvents / Q000494:pharmacology; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D003902:Detergents; D004800:Enzymes, Immobilized; D008670:Metals; D009930:Organic Chemicals; D012997:Solvents; D000681:Amylases