title: Continuous regional myocardial blood flow measurement: validation of a near-infrared laser Doppler device in a porcine model.
abstract: OBJECTIVE
  RMBF measurement is a major concern in various clinical and experimental settings, but no validated device for RMBF is currently available.
  
  
  METHODS
  An LVP-triggered laser Doppler to measure RMBF was validated by simultaneous fluorescent MS RMBF in a porcine LAD flow reduction model (n = 10 pigs). The laser probe was positioned on the left ventricle's anterior wall. LAD blood flow reduction was achieved by a shaft-driven occluder positioned proximal to the transit-time flow meter measuring coronary blood flow. RMBF was measured at baseline; after the reduction of LAD blood flow to 70% and 30% of baseline; at 20 and 120 minutes of reperfusion; and, finally, 15 minutes after LAD occlusion.
  
  
  RESULTS
  Laser Doppler RMBF (LDU) correlated strongly with MS RMBF under all tested conditions: baseline (epicardial 194.7 ± 41.9, endocardial 130.2 ± 29.2); 70% baseline-flow (epicardial 160.4 ± 27.7, endocardial 112.1 ± 15.1); 30% baseline-flow (epicardial 44.3 ± 5.5, endocardial 32.9 ± 9); 20 minutes reperfusion (epicardial 175.8 ± 33.6, endocardial 126.5 ± 30); 120 minutes reperfusion (epicardial 146.3 ± 31.1, endocardial 107.1 ± 29.7); and complete LAD occlusion (epicardial 10.5 ± 5.8 endocardial 1.4 ± 0.3) (r = 0.986-0.962, p < 0.001).
  
  
  CONCLUSIONS
  This new blood pressure waveform-triggered laser Doppler probe is able to measure RMBF at different depths online in the beating heart.
authors.affiliation: Department of Cardiovascular Surgery, University Hospital Kiel, Kiel, Germany. bier.bach@gmx.de
authors.forename: Benjamin
authors.initials: B
authors.lastname: Bierbach
authors.forename: Jens
authors.initials: J
authors.lastname: Scheewe
authors.forename: Thomas
authors.initials: T
authors.lastname: Derfuss
authors.forename: Alfons
authors.initials: A
authors.lastname: Krug
authors.forename: René
authors.initials: R
authors.lastname: Schramm
authors.forename: Manfred
authors.initials: M
authors.lastname: Dahm
authors.forename: Wlodzimierz
authors.initials: W
authors.lastname: Kuroczynski
authors.forename: Oliver
authors.initials: O
authors.lastname: Kempski
authors.forename: Georg
authors.initials: G
authors.lastname: Horstick
mesh_terms: D000818:Animals; D001783:Blood Flow Velocity / Q000502:physiology; D003331:Coronary Vessels / Q000502:physiology*; D017078:Laser-Doppler Flowmetry / Q000379:methods*; D008955:Models, Cardiovascular; D009206:Myocardium; D013552:Swine