title: [Interaction of SerpinB5 and MAFbx in gastric cancer cell and its action site].
abstract: OBJECTIVE
  To explore the interaction between SerpinB5 and MAFbx in gastric cancer cell and to identify the interaction sites.
  
  
  METHODS
  The interaction between SerpinB5 and MAFbx was screened and validated by yeast two-hybrid screening and co-immunoprecipitation. The expression of MAFbx was analyzed after SerpinB5 expression being modified by RNA interference and pGBKT7-SerpinB5 transfection. The impact of SerpinB5 on the expression of MAFbx was studied in gastric cancer cell line SUN-16. A model of MAFbx was constructed by homology modeling. The related residues for interaction were analyzed by Autodock4.0.
  
  
  RESULTS
  The interaction between SerpinB5 and MAFbx was validated. The expression of MAFbx changed along with SerpinB5 expression. Amino acids including PRO261, ASN361, and LYS362 were key residue in the interaction of SerpinB5 and MAFbx.
  
  
  CONCLUSIONS
  SerpinB5 interacts with MAFbx in gastric cancer cell. Amino acids including PRO261, ASN361, and LYS362 are potential binding sites.
authors.affiliation: Department of Surgery, Shanghai Jiaotong University School of Medicine, Shanghai, China.
authors.forename: Ke-feng
authors.initials: KF
authors.lastname: Lei
authors.forename: Yi-fei
authors.initials: YF
authors.lastname: Wang
authors.forename: Qun-qun
authors.initials: QQ
authors.lastname: Wang
authors.forename: Xiang-hong
authors.initials: XH
authors.lastname: He
authors.forename: Bing-ya
authors.initials: BY
authors.lastname: Liu
authors.forename: Xue-hua
authors.initials: XH
authors.lastname: Chen
authors.forename: Bei-qin
authors.initials: BQ
authors.lastname: Yu
authors.forename: Zheng-gang
authors.initials: ZG
authors.lastname: Zhu
mesh_terms: D045744:Cell Line, Tumor; D006801:Humans; D047468:Immunoprecipitation; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D044843:SKP Cullin F-Box Protein Ligases / Q000235:genetics / Q000378:metabolism*; D015843:Serpins / Q000235:genetics / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
chemical_list: D009124:Muscle Proteins; C085344:SERPIN-B5; D015843:Serpins; C440907:FBXO32 protein, human; D044843:SKP Cullin F-Box Protein Ligases