title: [Cloning and functional analysis of chitinase gene GbCHI from sea-island cotton (Gossypium barbadense)].
abstract: Chitinase is one of the important pathogenesis-related (PR) proteins in plants. By comparative proteomics study, a novel pathogen-responsive chitinase (known as GbCHI) has been identified from sea-island cotton (Gossypium barbadense). The GbCHI cDNA was cloned from wilt-resistant sea-island cotton and the anti-fungal activity of the gene product was investigated. qRT-PCR analysis indicated that GbCHI was expressed constitutively in root, stem, leaf, flower, and ovule of cotton plant, and the expression could be induced by Verticillium dahliae and plant hormone SA, ACC, and JA. Subcellular localization analysis using GFP-tagged proteins showed that GbCHI-GFP fusion proteins were targeted mainly to the plasma membrane. Anti-fungal assay demonstrated that GbCHI could inhibit spore germination and hyphae growth of V. dahliae significantly. These results provide important information for understanding the cellular function of GbCHI and for exploring the application potential of this gene in molecular breeding of wilt-tolerant cotton plants.
authors.affiliation: College of Agronomy, Shandong Agricultural University, Tai'an 271018, China. myp.apple@163.com
authors.forename: Yin-Ping
authors.initials: YP
authors.lastname: Ma
authors.forename: Fu-Xin
authors.initials: FX
authors.lastname: Wang
authors.forename: Chun-Lin
authors.initials: CL
authors.lastname: Yang
authors.forename: Fa-Fu
authors.initials: FF
authors.lastname: Shen
authors.forename: Gui-Xian
authors.initials: GX
authors.lastname: Xia
mesh_terms: D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000494:pharmacology; D002688:Chitinases / Q000737:chemistry / Q000235:genetics* / Q000494:pharmacology; D003001:Cloning, Molecular; D003368:Gossypium / Q000235:genetics*; D008969:Molecular Sequence Data; D040901:Proteomics; D020172:Verticillium / Q000187:drug effects
chemical_list: D000935:Antifungal Agents; D002688:Chitinases