title: Polymorphisms of SLC30A2 and selected perinatal factors associated with low milk zinc in Chinese breastfeeding women.
abstract: The variability of breast-milk zinc concentration is high among breastfeeding women, and it is known to be independent of dietary zinc intake. As a result, transient neonatal zinc deficiency is not rare in the breastfed infants due to low milk zinc concentration in their breastfeeding mothers. Up to now, SLC30A2 has been documented the only candidate gene showing correlation with human milk zinc trait. In this study, 750 breastfeeding women were recruited and 10ml foremilk was collected on 42nd postpartum day. The milk zinc concentration was measured, and genomic DNA was isolated from breast-milk. Direct sequencing and Taqman assay were used to identify the SLC30A2 polymorphisms associated with low-milk-zinc. Subsequently, the factors associated with breast-milk zinc were investigated using regression model. The correlation study showed that SLC30A2/-697G>T and SLC30A2/1031A>G polymorphisms were associated with low-milk-zinc in our subjects. These two polymorphisms explained 3.23% of total variance in milk zinc level. For non-genetic variables, the obese breastfeeding women (BMI>25) secreted less zinc into their breast-milk. The variation of milk zinc was independent of pregnant age, birth weight, infant gender, cesarean delivery, preterm delivery and vitamin D supplementation. In conclusion, our results indicated that -697G>T and 1031A>G polymorphisms in the SLC30A2 gene may be associated with low-milk-zinc in Chinese breastfeeding women. Maternal BMI is significantly correlated with milk zinc level in negative manner. Our study demonstrated that both genetic and non-genetic factors could modulate milk zinc level.
authors.affiliation: Xinhua Hospital, Shanghai Institute for Pediatric Research, Shanghai Jiao Tong University, School of Medicine, 1665 Kongjiang Road, Shanghai, China. microtuble@gmail.com
authors.forename: Linxi
authors.initials: L
authors.lastname: Qian
authors.forename: Bei
authors.initials: B
authors.lastname: Wang
authors.forename: Ning
authors.initials: N
authors.lastname: Tang
authors.forename: Weili
authors.initials: W
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Cai
mesh_terms: D000328:Adult; D001942:Breast Feeding; D027682:Cation Transport Proteins / Q000235:genetics*; D002681:China; D005260:Female; D006801:Humans; D008895:Milk, Human / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide; D049590:Postpartum Period / Q000378:metabolism; D015032:Zinc / Q000032:analysis / Q000172:deficiency*
chemical_list: D027682:Cation Transport Proteins; C486299:SLC30A2 protein, human; D015032:Zinc