title: [Effects of moxibustion on gastric mucosal EGF and TGF-alpha contents and epidermal growth factor receptor expression in rats with gastric mucosal lesion].
abstract: OBJECTIVE
  To observe the effects of moxibustion on contents of epidermal growth factor (EGF) and transforming growth factor-alpha (TGF-alpha) and epidermal growth factor receptor (EGFR) expression in the gastric mucosa tissue in rats with gastric mucosal lesion.
  
  
  METHODS
  Thirty-six SD rats were randomly and equally divided into control, model and moxibustion groups. Gastric mucosal lesion model was duplicated by restraint and cool water immersion stress. Pre-moxibustion was applied to "Zusanli" (ST 36) and "Zhongwan" (CV 12), "Pishu" (BL 20) and "Weishu" (BL 21) alternately, once everyday for 8 days before modeling. The contents of EGF and TGF-alpha in gastric mucosa were detected by enzyme-linked immunosorbent assay (ELISA) and the expression of EGFR determined by immunohistochemistry.
  
  
  RESULTS
  Compared with the control group, only TGF-alpha content in the gastric mucosa in the model group was increased significantly (P < 0.05). Compared with the model group, the EGF and TGF-alpha contents and EGFR immunoactivity in the gastric mucosa were increased significantly in the moxibustion group (P < 0.05, P < 0.01).
  
  
  CONCLUSIONS
  Pre-moxibustion at ST 36, CV 12, BL 20 and BL 21 can up-regulate gastric mucosal EGF and TGF-alpha contents and EGFR protein expression in gastric mucosa lesion rats, which may contribute to its effect in relieving stress-induced gastric mucosal injury.
authors.affiliation: Hong College of Acu-moxibustion and Tuina, Hunan University of Chinese Medicine, Changsha 410208, China.
authors.forename: Mi
authors.initials: M
authors.lastname: Liu
authors.forename: Xiao-Rong
authors.initials: XR
authors.lastname: Chang
authors.forename: Jie
authors.initials: J
authors.lastname: Yan
authors.forename: Shou-Xiang
authors.initials: SX
authors.lastname: Yi
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Lin
authors.forename: Zeng-Hui
authors.initials: ZH
authors.lastname: Yue
authors.forename: Yan
authors.initials: Y
authors.lastname: Peng
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000818:Animals; D004815:Epidermal Growth Factor / Q000378:metabolism*; D066246:ErbB Receptors / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005753:Gastric Mucosa / Q000293:injuries / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008297:Male; D009071:Moxibustion; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013272:Stomach Diseases / Q000235:genetics / Q000378:metabolism / Q000628:therapy*; D016211:Transforming Growth Factor alpha / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D016211:Transforming Growth Factor alpha; D004815:Epidermal Growth Factor; D066246:ErbB Receptors