title: DNA extraction from dried Schistosoma haematobium eggs isolated on nylon filters.
abstract: Genetic studies on Schistosoma haematobium are often carried out on DNA extracted from miracidia, cercariae or adult worms. This paper presents a method for extracting DNA from S. haematobium eggs collected from urine samples and stored on nylon filters at room temperature. DNA was extracted from dried S. haematobium eggs using the DNeasy Blood & Tissue Kit (QIAGEN Sample & Assay Technologies, Copenhagen, Denmark). Selected genes were amplified using PCR to verify that DNA extraction had been successful. DNA was extracted from 45 samples and 31 had a positive PCR reaction for either or both of the two selected genes.
authors.affiliation: DBL-Centre for Health Research and Development, Department of Veterinary Disease Biology, Faculty of Life Sciences, University of Copenhagen, Frederiksberg C, Denmark. linereinstrup@hotmail.com
authors.forename: L
authors.initials: L
authors.lastname: Reinstrup
authors.forename: A
authors.initials: A
authors.lastname: Jørgensen
authors.forename: B J
authors.initials: BJ
authors.lastname: Vennervald
authors.forename: T K
authors.initials: TK
authors.lastname: Kristensen
mesh_terms: D000818:Animals; D002648:Child; D018509:DNA, Helminth / Q000302:isolation & purification*; D005260:Female; D005374:Filtration; D006801:Humans; D008297:Male; D009073:Mozambique / Q000453:epidemiology; D010270:Parasite Egg Count; D016133:Polymerase Chain Reaction / Q000295:instrumentation*; D012548:Schistosoma haematobium / Q000235:genetics* / Q000302:isolation & purification; D012553:Schistosomiasis haematobia / Q000453:epidemiology / Q000652:urine; D012574:Schools; D012680:Sensitivity and Specificity; D013045:Species Specificity; D013334:Students / Q000706:statistics & numerical data
chemical_list: D018509:DNA, Helminth