title: A single amino acid in the helicase domain of PMMoV-S is responsible for its enhanced accumulation in C. chinense (L(3)L(3)) plants at 32°C.
abstract: In Capsicum chinense (L(3)L(3)) plants a higher accumulation of the tobamovirus Pepper mild mottle virus strain S (PMMoV-S) as compared to the Italian strain PMMoV-I is detected when plants are grown at 32°C. By using a reverse genetic approach, we have established that a single amino acid at position 898 in the helicase domain of the polymerase protein, outside of the conserved regions of the helicase, is critical for the higher accumulation of PMMoV-S observed. It also is necessary for both increased accumulation of viral RNA of both polarities in pepper protoplasts and enhanced cell-to-cell movement in C. chinense plants. The influence of thermoresistance of PMMoV-S, a P(1,2) pathotype, and its prevalence on pepper cultivars over PMMoV-I, a P(1,2,3), pathotype, is discussed.
authors.affiliation: Departamento de Biología Medioambiental, Centro de Investigaciones Biológicas, CSIC, C/Ramiro de Maeztu no 9, 28040 Madrid, Spain.
authors.forename: Fátima
authors.initials: F
authors.lastname: Tena
authors.forename: Myriam
authors.initials: M
authors.lastname: Molina-Galdeano
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Serra
authors.forename: Isabel
authors.initials: I
authors.lastname: García-Luque
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001692:Biological Transport; D002212:Capsicum / Q000737:chemistry / Q000378:metabolism / Q000821:virology*; D004265:DNA Helicases / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism / Q000821:virology; D012367:RNA, Viral / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013696:Temperature; D017898:Tobamovirus / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D014774:Virulence / Q000235:genetics
chemical_list: D012367:RNA, Viral; D004265:DNA Helicases