title: Anatase-based implants nanocoating on stem cells derived from adipose tissue.
abstract: OBJECTIVE
  The aim of this study was to investigate the effect of a new anatase coating with antibacterial properties (Bactercline anatase coating [BAC]) on dental implants in the commitment of stem cells derived from adipose tissue to osteoblasts.
  
  
  METHODS
  Using real-time reverse transcription polymerase chain reaction, the quantitative expression of specific genes, such as transcriptional factors (runx2 and sp7), bone-related genes (spp1, col1a1, col3a1, alpl, and fosl1), and mesenchymal stem cells marker (eng), was examined.
  
  
  RESULTS
  BAC caused induction of bone-related genes such as sp7, fosl1, alpl, and spp1. In contrast, the expression of runx2, col3a1, and col1a1 was decreased in stem cells treated with BAC with respect to untreated cells.
  
  
  CONCLUSIONS
  The obtained results are relevant to better understand the molecular mechanism of bone regeneration and as a model for comparing other materials with similar clinical effects.
authors.affiliation: Department of Maxillofacial Surgery, University of Ferrara, Ferrara, Italy.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Zollino
authors.forename: Ambra
authors.initials: A
authors.lastname: Girardi
authors.forename: Annalisa
authors.initials: A
authors.lastname: Palmieri
authors.forename: Francesca
authors.initials: F
authors.lastname: Cura
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Sollazzo
authors.forename: Giorgio
authors.initials: G
authors.lastname: Brunelli
authors.forename: Francesco
authors.initials: F
authors.lastname: Carinci
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D000469:Alkaline Phosphatase / Q000032:analysis; D015703:Antigens, CD / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D001854:Bone Marrow Cells / Q000166:cytology; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D024042:Collagen Type I / Q000032:analysis; D000090602:Collagen Type I, alpha 1 Chain; D024061:Collagen Type III / Q000032:analysis; D050659:Core Binding Factor Alpha 1 Subunit / Q000032:analysis; D015921:Dental Implants; D000071063:Endoglin; D006801:Humans; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology; D049329:Nanostructures / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology; D010012:Osteogenesis / Q000502:physiology; D053495:Osteopontin / Q000032:analysis; D016760:Proto-Oncogene Proteins c-fos / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction; D011956:Receptors, Cell Surface / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000076182:Sp7 Transcription Factor; D013234:Stem Cells / Q000166:cytology*; D017154:Stromal Cells / Q000166:cytology; D014025:Titanium / Q000737:chemistry*; D014157:Transcription Factors / Q000032:analysis; D000099169:Fos-Related Antigen 1
chemical_list: D000469:Alkaline Phosphatase; D015703:Antigens, CD; D015415:Biomarkers; D020099:Coated Materials, Biocompatible; D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; D024061:Collagen Type III; D050659:Core Binding Factor Alpha 1 Subunit; D015921:Dental Implants; D000071063:Endoglin; D053495:Osteopontin; D016760:Proto-Oncogene Proteins c-fos; D011956:Receptors, Cell Surface; D000076182:Sp7 Transcription Factor; D014025:Titanium; D014157:Transcription Factors; D000099169:Fos-Related Antigen 1; C439971:COL3A1 protein, human; C064206:ENG protein, human; C493709:RUNX2 protein, human; C506895:SPP1 protein, human; C478163:SP7 protein, human; C009495:titanium dioxide; C548267:ALPL protein, human