title: [Effects of erythropoietin pretreatment on pro-and anti-inflammatory balance in rats with severe acute pancreatitis].
abstract: OBJECTIVE
  To evaluate the effect of pretreatment with erythropoietin (EPO) on disordered pro- and anti- inflammatory balance in rats with severe acute pancreatitis (SAP) and explore the underlying mechanisms.
  
  
  METHODS
  Ninety healthy male SD rats were randomized equally into sham-operated group, SAP group and EPO pretreatment group. SAP model was induced in the latter two groups by retrograde injection of 1 ml/kg 3.5% sodium traurocholate into the biliopancreatic duct. In EPO group, 3000 U/kg EPO (1000 U/ml) was administered intravenously 1 h before SAP, and normal saline was administered in the other two groups. Serum amylase activity, interleukin-10 (IL-10)and IL-18 levels were measured at different time points after the operation. The translocation and activation of nuclear factor-κB (NF-κB) in the pancreatic tissue was detected using immunofluorescence staining, and pancreatic pathologies were evaluated.
  
  
  RESULTS
  Compared with SAP group, EPO group showed a markedly decreased activation rate of NF-κB after SAP except for 12 h (P<0.05), significantly decreased serum amylase activity at 3, 6, and 12 h (P<0.05) and decreased serum IL-18 levels at 3, 6, 24 h (P<0.05), whereas serum IL-10 underwent no significant changes. The rats in EPO group showed an obviously milder pancreatic pathology than those in SAP group at 6, 12, and 24 h (P<0.05).
  
  
  CONCLUSIONS
  EPO can effectively inhibit NF-κB activation by regulating the inflammatory mediators and restoring the pro-and anti-inflammatory balance to alleviate SAP in rats.
authors.affiliation: Department of Hepatobiliary Surgery, First Affiliated Hospital of Jinan University, Guangzhou, China. 786696877@qq.com
authors.forename: Jiexing
authors.initials: J
authors.lastname: Li
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Luo
authors.forename: Zhendong
authors.initials: Z
authors.lastname: Li
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhaoxiang
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000208:Acute Disease; D000818:Animals; D016207:Cytokines / Q000378:metabolism*; D004921:Erythropoietin / Q000627:therapeutic use*; D016753:Interleukin-10 / Q000378:metabolism; D020382:Interleukin-18 / Q000378:metabolism; D008297:Male; D016328:NF-kappa B / Q000378:metabolism; D010195:Pancreatitis / Q000188:drug therapy*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D016207:Cytokines; D020382:Interleukin-18; D016328:NF-kappa B; D004921:Erythropoietin; D016753:Interleukin-10