title: Detection of protein deposition within latent fingerprints by surface-enhanced Raman spectroscopy imaging.
abstract: The detection of metabolites is very important for the estimation of the health of human beings. Latent fingerprint contains many constituents and specific contaminants, which give much information of the individual, such as health status, drug abuse etc. For a long time, many efforts have been focused on visualizing latent fingerprints, but little attention has been paid to the detection of such substances at the same time. In this article, we have devised a versatile approach for the ultra-sensitive detection and identification of specific biomolecules deposited within fingerprints via a large-area SERS imaging technique. The antibody bound to the Raman probe modified silver nanoparticles enables the binding to specific proteins within the fingerprints to afford high-definition SERS images of the fingerprint pattern. The SERS spectra and images of Raman probes indirectly provide chemical information regarding the given proteins. By taking advantage of the high sensitivity and the capability of SERS technique to obtain abundant vibrational signatures of biomolecules, we have successfully detected minute quantities of protein present within a latent fingerprint. This technique provides a versatile and effective model to detect biomarkers within fingerprints for medical diagnostics, criminal investigation and other fields.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun 130022, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Song
authors.forename: Zhu
authors.initials: Z
authors.lastname: Mao
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Liu
authors.forename: Yong
authors.initials: Y
authors.lastname: Lu
authors.forename: Zhishi
authors.initials: Z
authors.lastname: Li
authors.forename: Bing
authors.initials: B
authors.lastname: Zhao
authors.forename: Lehui
authors.initials: L
authors.lastname: Lu
mesh_terms: D000327:Adsorption; D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000378:metabolism; D002417:Cattle; D003878:Dermatoglyphics; D006041:Goats; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry / Q000378:metabolism; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D008954:Models, Biological; D058726:Nanoconjugates / Q000737:chemistry; D011506:Proteins / Q000493:pharmacokinetics*; D012834:Silver / Q000737:chemistry; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*; D013499:Surface Properties
chemical_list: D000906:Antibodies; D007074:Immunoglobulin G; D058726:Nanoconjugates; D011506:Proteins; D012834:Silver