title: Expression and prognostic relevance of PRAME in primary osteosarcoma.
abstract: The preferentially expressed antigen of melanoma (PRAME), a cancer-testis antigen with unknown function, is expressed in many human malignancies and is considered an attractive potential target for tumor immunotherapy. However, studies of its expression and function in osteosarcoma have rarely been reported. In this study, we found that PRAME is expressed in five osteosarcoma cell lines and in more than 70% of osteosarcoma patient specimens. In addition, an immunohistochemical analysis showed that high PRAME expression was associated with poor prognosis and lung metastasis. Furthermore, PRAME siRNA knockdown significantly suppressed the proliferation, colony formation, and G1 cell cycle arrest in U-2OS cells. Our results suggest that PRAME plays an important role in cell proliferation and disease progression in osteosarcoma. However, the detail mechanisms of PRAME function in osteosarcoma require further investigation.
authors.affiliation: Department of Orthopaedic Surgery, Musculoskeletal Tumor Center, First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, China.
authors.forename: Pingxian
authors.initials: P
authors.lastname: Tan
authors.forename: Changye
authors.initials: C
authors.lastname: Zou
authors.forename: Bicheng
authors.initials: B
authors.lastname: Yong
authors.forename: Ju
authors.initials: J
authors.lastname: Han
authors.forename: Longjuan
authors.initials: L
authors.lastname: Zhang
authors.forename: Qiao
authors.initials: Q
authors.lastname: Su
authors.forename: Junqiang
authors.initials: J
authors.lastname: Yin
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.forename: Gang
authors.initials: G
authors.lastname: Huang
authors.forename: Tingsheng
authors.initials: T
authors.lastname: Peng
authors.forename: Jingnian
authors.initials: J
authors.lastname: Shen
mesh_terms: D000951:Antigens, Neoplasm / Q000096:biosynthesis* / Q000235:genetics; D001859:Bone Neoplasms / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D055785:Gene Knockdown Techniques; D006801:Humans; D012516:Osteosarcoma / Q000378:metabolism / Q000473:pathology*; D011379:Prognosis; D034741:RNA, Small Interfering / Q000235:genetics; D014162:Transfection
chemical_list: D000951:Antigens, Neoplasm; C404944:PRAME protein, human; D034741:RNA, Small Interfering