title: Cyclin-dependent kinase inhibitor 3 is overexpressed in hepatocellular carcinoma and promotes tumor cell proliferation.
abstract: Cyclin-dependent kinase inhibitor 3 (CDKN3) belongs to the protein phosphatases family and has a dual function in cell cycling. The function of this gene has been studied in several kinds of cancers, but its role in human hepatocellular carcinoma (HCC) remains to be elucidated. In this study, we found that CDKN3 was frequently overexpressed in both HCC cell lines and clinical samples, and this overexpression was correlated with poor tumor differentiation and advanced tumor stage. Functional studies showed that overexpression of CDKN3 could promote cell proliferation by stimulating G1-S transition but has no impact on cell apoptosis and invasion. Microarray-based co-expression analysis identified a total of 61 genes co-expressed with CDKN3, with most of them involved in cell proliferation, and BIRC5 was located at the center of CDKN3 co-expression network. These results suggest that CDKN3 acts as an oncogene in human hepatocellular carcinoma and antagonism of CDKN3 may be of interest for the treatment of HCC.
authors.affiliation: Key Lab of Combined Multi-Organ Transplantation, Ministry of Public Health, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, Zhejiang Province, China.
authors.forename: Chunyang
authors.initials: C
authors.lastname: Xing
authors.forename: Haiyang
authors.initials: H
authors.lastname: Xie
authors.forename: Lin
authors.initials: L
authors.lastname: Zhou
authors.forename: Wuhua
authors.initials: W
authors.lastname: Zhou
authors.forename: Wu
authors.initials: W
authors.lastname: Zhang
authors.forename: Songming
authors.initials: S
authors.lastname: Ding
authors.forename: Bajin
authors.initials: B
authors.lastname: Wei
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Yu
authors.forename: Rong
authors.initials: R
authors.lastname: Su
authors.forename: Shusen
authors.initials: S
authors.lastname: Zheng
mesh_terms: D000328:Adult; D006528:Carcinoma, Hepatocellular / Q000201:enzymology* / Q000473:pathology*; D049109:Cell Proliferation; D050756:Cyclin-Dependent Kinase Inhibitor Proteins / Q000096:biosynthesis* / Q000235:genetics; D054637:Dual-Specificity Phosphatases / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D006801:Humans; D008113:Liver Neoplasms / Q000201:enzymology* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D055815:Young Adult
chemical_list: D050756:Cyclin-Dependent Kinase Inhibitor Proteins; C084429:CDKN3 protein, human; D054637:Dual-Specificity Phosphatases