title: Drosophila as a model for MECP2 gain of function in neurons.
abstract: Methyl-CpG-binding protein 2 (MECP2) is a multi-functional regulator of gene expression. In humans loss of MECP2 function causes classic Rett syndrome, but gain of MECP2 function also causes mental retardation. Although mouse models provide valuable insight into Mecp2 gain and loss of function, the identification of MECP2 genetic targets and interactors remains time intensive and complicated. This study takes a step toward utilizing Drosophila as a model to identify genetic targets and cellular consequences of MECP2 gain-of function mutations in neurons, the principle cell type affected in patients with Rett-related mental retardation. We show that heterologous expression of human MECP2 in Drosophila motoneurons causes distinct defects in dendritic structure and motor behavior, as reported with MECP2 gain of function in humans and mice. Multiple lines of evidence suggest that these defects arise from specific MECP2 function. First, neurons with MECP2-induced dendrite loss show normal membrane currents. Second, dendritic phenotypes require an intact methyl-CpG-binding domain. Third, dendritic defects are amended by reducing the dose of the chromatin remodeling protein, osa, indicating that MECP2 may act via chromatin remodeling in Drosophila. MECP2-induced motoneuron dendritic defects cause specific motor behavior defects that are easy to score in genetic screening. In sum, our data show that some aspects of MECP2 function can be studied in the Drosophila model, thus expanding the repertoire of genetic reagents that can be used to unravel specific neural functions of MECP2. However, additional genes and signaling pathways identified through such approaches in Drosophila will require careful validation in the mouse model.
authors.affiliation: School of Life Sciences, Arizona State University, Tempe, Arizona, United States of America.
authors.forename: Fernando
authors.initials: F
authors.lastname: Vonhoff
authors.forename: Alison
authors.initials: A
authors.lastname: Williams
authors.forename: Stefanie
authors.initials: S
authors.lastname: Ryglewski
authors.forename: Carsten
authors.initials: C
authors.lastname: Duch
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D002462:Cell Membrane / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D003712:Dendrites / Q000378:metabolism; D029721:Drosophila Proteins / Q000378:metabolism; D004331:Drosophila melanogaster / Q000166:cytology / Q000378:metabolism*; D055724:Electrophysiological Phenomena; D006801:Humans; D051783:Methyl-CpG-Binding Protein 2 / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D023421:Models, Animal; D009043:Motor Activity; D009046:Motor Neurons / Q000166:cytology / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D017434:Protein Structure, Tertiary
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; C497392:MECP2 protein, human; D051783:Methyl-CpG-Binding Protein 2; C117995:osa protein, Drosophila