title: Identification of clinical isolates of anaerobic bacteria using matrix-assisted laser desorption ionization-time of flight mass spectrometry.
abstract: We evaluated the use of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF) for the rapid identification of anaerobic bacteria that had been isolated from clinical specimens and previously identified by 16s rRNA sequencing. The Bruker Microflex MALDI-TOF instrument with the Biotyper Software was used. We tested 152 isolates of anaerobic bacteria from 24 different genera and 75 different species. A total of 125 isolates (82%) had Biotyper software scores greater than 2.0 and the correct identification to genus and species was made by MALDI-TOF for 120 (79%) of isolates. Of the 12 isolates with a score between 1.8 and 2.0, 2 (17%) organisms were incorrectly identified by MALDI-TOF. Only 15 (10%) isolates had a score less than 1.8 and MALDI-TOF gave the wrong genus and species for four isolates, the correct genus for two isolates, and the correct genus and species for nine isolates. Therefore, we found the Bruker MALDI-TOF MicroFlex LT with an expanded database and the use of bacteria extracts rather than whole organisms correctly identified 130 of 152 (86%) isolates to genus and species when the cut-off for an acceptable identification was a spectrum score ≥1.8.
authors.affiliation: Department of Laboratory Medicine, Clinical Center, Nationals Institutes of Health, Microbiology Service, Bldg 10/Rm 2C385, 10 Center Drive MSC 1508, Bethesda, MD, 20892-1508, USA. dfedorko@nih.gov
authors.forename: D P
authors.initials: DP
authors.lastname: Fedorko
authors.forename: S K
authors.initials: SK
authors.lastname: Drake
authors.forename: F
authors.initials: F
authors.lastname: Stock
authors.forename: P R
authors.initials: PR
authors.lastname: Murray
mesh_terms: D001421:Bacteria, Anaerobic / Q000737:chemistry* / Q000145:classification* / Q000302:isolation & purification; D001424:Bacterial Infections / Q000175:diagnosis*; D001431:Bacteriological Techniques / Q000379:methods*; D006801:Humans; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*