title: Bi-stage control of dissolved oxygen to enhance cyclic adenosine monophosphate production by Arthrobacter A302.
abstract: Experiments confirmed dissolved oxygen (DO) definitely affects cyclic adenosine monophosphate (cAMP) production by Arthrobacter A302. Production of cAMP by batch fermentation was investigated under various DO conditions. A two-stage DO control strategy was proposed to achieve optimal production of cAMP based on the kinetic analysis: the DO level was controlled at 40% during the first 18 h and then switched to 30%. Relatively high cAMP production (9.9 g L(-1)) was achieved by applying this strategy. The cAMP productivity (0.14 g L(-1) h(-1)) was also successfully improved by 85.1, 59.3, 15.1 and 28.0%, compared to cases in which DO was uncontrolled or DO levels were held at 20, 30 and 40%, respectively. This is the first report of the use of a two-stage DO control strategy in cAMP production, and it was verified to be an effective method for enhancing the cAMP yield via this strain.
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Chen
authors.forename: Jian
authors.initials: J
authors.lastname: Cheng
authors.forename: Chunwei
authors.initials: C
authors.lastname: Zhang
authors.forename: Jianxin
authors.initials: J
authors.lastname: Bai
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
authors.forename: Huanqin
authors.initials: H
authors.lastname: Niu
authors.forename: Hanjie
authors.initials: H
authors.lastname: Ying
mesh_terms: D001173:Arthrobacter / Q000254:growth & development* / Q000378:metabolism*; D000242:Cyclic AMP / Q000096:biosynthesis*; D010100:Oxygen / Q000378:metabolism*; D010101:Oxygen Consumption
chemical_list: D000242:Cyclic AMP; D010100:Oxygen