title: Longitudinal assessment of two Mycoplasma hyopneumoniae enzyme-linked immunosorbent assays in challenged and contact-exposed pigs.
abstract: The objective of the current study was to compare the sensitivity of 2 Mycoplasma hyopneumoniae enzyme-linked immunosorbent assays (ELISAs) in experimentally challenged and contact-exposed pigs in a long-term longitudinal assessment. On day 0 of the assessment, twelve 2-month-old M. hyopneumoniae-negative pigs were inoculated with M. hyopneumoniae strain 232 (group A). Twelve negative pigs were placed alongside the inoculated pigs, allowing direct contact exposure (group B). A third group of 12 pigs was allocated into 2 independent pens; no direct contact was allowed (group C). A longitudinal serologic profile was performed; samples were collected on days 0, 28, 35, 42, 49, 63, 91, 119, 154, and 170 of the study. Serum samples were tested using a blocking ELISA and an indirect ELISA. Results of the study demonstrated higher sensitivity of the blocking ELISA during early infection (clinical signs). Both ELISAs were 100% sensitive in challenged and naturally infected groups at several testing points during late infection (63, 91, 119, 154, and 170 days of the study) and showed a long antibody detection period. Both tests worked equally well during the chronic phase of infection but the blocking ELISA was more sensitive during acute stages of infection.
authors.affiliation: PIC, Queretaro, Mexico.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Fano
authors.forename: Carlos
authors.initials: C
authors.lastname: Pijoan
authors.forename: Scott
authors.initials: S
authors.lastname: Dee
authors.forename: John
authors.initials: J
authors.lastname: Deen
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D008137:Longitudinal Studies; D045705:Mycoplasma hyopneumoniae / Q000302:isolation & purification*; D045729:Pneumonia of Swine, Mycoplasmal / Q000097:blood / Q000382:microbiology*; D012680:Sensitivity and Specificity; D013552:Swine
chemical_list: D000907:Antibodies, Bacterial