title: Enhancement of immune response towards non-lipidized Borrelia burgdorferi recombinant OspC antigen by binding onto the surface of metallochelating nanoliposomes with entrapped lipophilic derivatives of norAbuMDP.
abstract: Lyme disease caused by spirochete Borrelia burgdorferi sensu lato, is a tick-born illness. If the infection is not eliminated by the host immune system and/or antibiotics, it may further disseminate and cause severe chronic complications. The immune response to Borrelia is mediated by phagocytic cells and by Borrelia-specific complement-activating antibodies associated with Th1 cell activation. A new experimental vaccine was constructed using non-lipidized form of recombinant B. burgdorferi s.s. OspC protein was anchored by metallochelating bond onto the surface of nanoliposomes containing novel nonpyrogenic lipophilized norAbuMDP analogues denoted MT05 and MT06. After i.d. immunization, the experimental vaccines surpassed Alum with respect to OspC-specific titers of IgG2a, IgG2b isotypes when MT06 was used and IgG3, IgM isotypes when MT05 was used. Both adjuvants exerted a high adjuvant effect comparable or better than MDP and proved themselves as nonpyrogenic.
authors.affiliation: Department of Immunology, Palacky University in Olomouc, Czech Republic.
authors.forename: Michal
authors.initials: M
authors.lastname: Křupka
authors.forename: Josef
authors.initials: J
authors.lastname: Mašek
authors.forename: Eliška
authors.initials: E
authors.lastname: Bartheldyová
authors.forename: Pavlína
authors.initials: P
authors.lastname: Turánek Knötigová
authors.forename: Jana
authors.initials: J
authors.lastname: Plocková
authors.forename: Zina
authors.initials: Z
authors.lastname: Korvasová
authors.forename: Michaela
authors.initials: M
authors.lastname: Škrabalová
authors.forename: Štěpán
authors.initials: Š
authors.lastname: Koudelka
authors.forename: Pavel
authors.initials: P
authors.lastname: Kulich
authors.forename: Kateřina
authors.initials: K
authors.lastname: Zachová
authors.forename: Lýdie
authors.initials: L
authors.lastname: Czerneková
authors.forename: Ondřej
authors.initials: O
authors.lastname: Strouhal
authors.forename: Milada
authors.initials: M
authors.lastname: Horynová
authors.forename: Marek
authors.initials: M
authors.lastname: Šebela
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Miller
authors.forename: Miroslav
authors.initials: M
authors.lastname: Ledvina
authors.forename: Milan
authors.initials: M
authors.lastname: Raška
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Turánek
mesh_terms: D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000737:chemistry* / Q000633:toxicity; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D025065:Borrelia burgdorferi / Q000276:immunology*; D002152:Calorimetry, Differential Scanning; D002614:Chelating Agents / Q000737:chemistry* / Q000633:toxicity; D004337:Drug Carriers / Q000737:chemistry* / Q000633:toxicity; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D008027:Light; D008081:Liposomes; D022123:Lyme Disease Vaccines / Q000008:administration & dosage / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D012542:Scattering, Radiation; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D002614:Chelating Agents; D004337:Drug Carriers; D008081:Liposomes; D022123:Lyme Disease Vaccines; C079616:OspC protein; D000119:Acetylmuramyl-Alanyl-Isoglutamine