title: Influence of an immunopotentiator Polyoxidonium on cytokine profile and antibody production in children vaccinated with Priorix.
abstract: 60 children aged 1-2 years old (32 boys and 28 girls) were vaccinated with Priorix. Vaccinated children included healthy control (19 children, group 1), and children with immunological disturbances such as episodes of respiratory infection. From the latter group, 20 children did not receive (group 2), and 21 children received 0.15 mg/kg of Polyoxidonium simultaneously with the vaccine (group 3).On days 7 and 30 after vaccination, CD-markers on lymphocytes and concentration of specific antibodies, as well as levels of 11 cytokines in serum were evaluated by flow cytometry, ELISA, and multiplex techniques respectively. It was found that injection of Polyoxidonium skewed T helper differentiation to Th2 type. Antibody responses were significantly higher in children with preferable Th2 responses. Children from group 3 possessed higher titers of specific IgG-antibodies. Our study shows that Polyoxidonium could smooth out the immune reaction on vaccination. It is important for children with some immunological disturbances.
authors.affiliation: G.N. Gabrichevsky Institute of Epidemiology and Microbiology, Moscow, Russia. toptyginaanna@rambler.ru
authors.forename: Anna
authors.initials: A
authors.lastname: Toptygina
authors.forename: Elena
authors.initials: E
authors.lastname: Semikina
authors.forename: Vladimir
authors.initials: V
authors.lastname: Alioshkin
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000097:blood / Q000276:immunology; D002675:Child, Preschool; D016207:Cytokines / Q000097:blood*; D005260:Female; D006801:Humans; D007223:Infant; D016131:Lymphocyte Subsets / Q000187:drug effects / Q000276:immunology; D008297:Male; D022542:Measles-Mumps-Rubella Vaccine / Q000276:immunology*; D010879:Piperazines / Q000494:pharmacology*; D011108:Polymers / Q000494:pharmacology*; D014611:Vaccination; D014613:Vaccines, Attenuated / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D016207:Cytokines; D022542:Measles-Mumps-Rubella Vaccine; D010879:Piperazines; D011108:Polymers; D014613:Vaccines, Attenuated; C540909:azoximer bromide