title: [Intratumor injection of  recombinant  attenuated salmonella carrying Mycobacterium tuberculosis heat shock protein 70 and herpes simplex virus thymidine kinase genes to suppress murine melanoma growth].
abstract: OBJECTIVE
  To study the effection of suppression murine melanoma growth by Intratumor injection of recombinant  attenuated salmonella carrying heat shock protein 70 and herpes simplex virus thymidine kinase genes.
  
  
  METHODS
  Plasmids PCMV-mtHSP70-IRES-TK  were electro-transferred into salmonella typhimurium SL7207 to construct recombinant salmonella typhimurium. In vivo, Recombinant bacteria were  injected into the mouse melanoma and the antitumor effection was observed. The survival period was recorded and safety analysis for this vaccine in each group.
  
  
  RESULTS
  In vivo, the mtHSP70/HSV-tk recombinant bacteria can suppress tumor growth significantly and extend survival. After recombinant Salmonella, 10(9) CFU/mL, was administered as an intratumoral injection, No diarrhea  were observed. During therapy, body weight did not change markedly.
  
  
  CONCLUSIONS
  Results of the animal experiment suggests intratumor injection of recombinant attenuated salmonella typhimurium containing mtHSP70 and HSV-tk genes, has targeting ability against B16 tumor cell and could significantly inhibit tumor growth .
authors.affiliation: Department of Oral and Maxillofacial Surgery, Southern Medical University, Guangzhou, China. sunrisez@tom.com
authors.forename: Shuguang
authors.initials: S
authors.lastname: Zeng
authors.forename: Qicai
authors.initials: Q
authors.lastname: Liu
authors.forename: Suwen
authors.initials: S
authors.lastname: Wang
authors.forename: Ximao
authors.initials: X
authors.lastname: Peng
authors.forename: Jincai
authors.initials: J
authors.lastname: Zhang
authors.forename: Jiren
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology; D019496:Cancer Vaccines / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D015316:Genetic Therapy / Q000379:methods; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000276:immunology*; D008546:Melanoma, Experimental / Q000382:microbiology / Q000473:pathology / Q000628:therapy*; D051379:Mice; D008810:Mice, Inbred C57BL; D009169:Mycobacterium tuberculosis / Q000235:genetics; D012486:Salmonella typhimurium / Q000235:genetics / Q000276:immunology; D018139:Simplexvirus / Q000201:enzymology* / Q000235:genetics; D012878:Skin Neoplasms / Q000628:therapy; D013937:Thymidine Kinase / Q000235:genetics / Q000276:immunology*; D014613:Vaccines, Attenuated / Q000235:genetics / Q000276:immunology / Q000494:pharmacology; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; D019496:Cancer Vaccines; D018840:HSP70 Heat-Shock Proteins; D014613:Vaccines, Attenuated; D019444:Vaccines, DNA; D013937:Thymidine Kinase