title: Proteomic study of the yeast Rhodotorula mucilaginosa RCL-11 under copper stress.
abstract: In order to understand the mechanism involved in Rhodotorula mucilaginosa RCL-11 resistance to copper a proteomic study was conducted. Atomic absorption spectroscopy showed that the copper concentration in the medium decreased from 0.5 to 0.19 mM 48 h after inoculation of the yeast. Analysis of one-dimensional gel electrophoresis of crude cell extracts revealed expression of differential bands between cells with and without copper. In order to study this difference, two-dimensional electrophoresis of R. mucilaginosa RCL-11 exposed to Cu for 16, 24, and 48 h was carried out. Identification of differentially expressed proteins was performed by MALDI-TOF/TOF. Ten of the 16 spots identified belonged to heat shock proteins. Superoxide dismutase, methionine synthase and beta-glucosidase were also found over-expressed at high copper concentrations. The results obtained in the present work show that when R. mucilaginosa RCL-11 is exposed to 0.5 mM copper, differential proteins, involved in cell resistance mechanisms, are expressed.
authors.affiliation: Planta Piloto de Procesos Industriales Microbiológicos, PROIMI-CONICET, Av. Belgrano y Pje. Caseros, T4001MVB Tucumán, Argentina. veronicairazusta@hotmail.com
authors.forename: Verónica
authors.initials: V
authors.lastname: Irazusta
authors.forename: Cristina
authors.initials: C
authors.lastname: Estévez
authors.forename: María Julia
authors.initials: MJ
authors.lastname: Amoroso
authors.forename: Lucía I C
authors.initials: LI
authors.lastname: de Figueroa
mesh_terms: D013766:5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase / Q000378:metabolism; D001673:Biodegradation, Environmental; D003300:Copper / Q000494:pharmacology*; D015180:Electrophoresis, Gel, Two-Dimensional; D040901:Proteomics / Q000379:methods*; D012248:Rhodotorula / Q000187:drug effects / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: D003300:Copper; D013482:Superoxide Dismutase; D013766:5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase; D001617:beta-Glucosidase