title: HWGMWSY, an unanticipated polystyrene binding peptide from random phage display libraries.
abstract: Phage display is a powerful technique for the discovery of peptide ligands that bind to various targets; however, ambiguous results often appear. Peptide HWGMWSY has been isolated repeatedly in our laboratory and by other research groups dealing with different protein and nonprotein targets, which led to a hypothesis that it may be a target-unrelated peptide interacting with polystyrene plastic surfaces. We compared binding properties and amplification rate of phage clone displaying the peptide HWGMWSY, a previously confirmed plastic binding clone WHWRLPS, and a control phage clone ASVQERK. An enzyme-linked immunosorbent assay and a phage elution assay confirmed that phage clone HWGMWSY binds to polystyrene. Surface plasmon resonance measurements on the other hand excluded the possibility of binding to bovine serum albumin, a common blocking agent in phage display experiments. Amplification rates of the above-noted phage clones were not statistically different. We therefore conclude that phage clone HWGMWSY was isolated in different selection procedures as a result of its affinity to polystyrene.
authors.affiliation: Department of Pharmaceutical Biology, Faculty of Pharmacy, Ljubljana, Slovenia. miha.vodnik@ffa.uni-lj.si
authors.forename: Miha
authors.initials: M
authors.lastname: Vodnik
authors.forename: Borut
authors.initials: B
authors.lastname: Strukelj
authors.forename: Mojca
authors.initials: M
authors.lastname: Lunder
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002999:Clone Cells; D004797:Enzyme-Linked Immunosorbent Assay; D054439:Ghrelin / Q000737:chemistry; D008024:Ligands; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D019151:Peptide Library; D011137:Polystyrenes / Q000737:chemistry*; D011485:Protein Binding; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000737:chemistry; D020349:Surface Plasmon Resonance; D053482:beta 2-Glycoprotein I / Q000737:chemistry
chemical_list: D054439:Ghrelin; D008024:Ligands; D009842:Oligopeptides; D019151:Peptide Library; D011137:Polystyrenes; D053482:beta 2-Glycoprotein I; D012710:Serum Albumin, Bovine