title: Fhl1 as a downstream target of Wnt signaling to promote myogenesis of C2C12 cells.
abstract: Previous studies have shown that Wnt signaling is involved in postnatal mammalian myogenesis; however, the downstream mechanism of Wnt signaling is not fully understood. This study reports that the murine four-and-a-half LIM domain 1 (Fhl1) could be stimulated by β-catenin or LiCl treatment to induce myogenesis. In contrast, knockdown of the Fhl1 gene expression in C2C12 cells led to reduced myotube formation. We also adopted reporter assays to demonstrate that either β-catenin or LiCl significantly activated the Fhl1 promoter, which contains four putative consensus TCF/LEF binding sites. Mutations of two of these sites caused a significant decrease in promoter activity by luciferase reporter assay. Thus, we suggest that Wnt signaling induces muscle cell differentiation, at least partly, through Fhl1 activation.
authors.affiliation: Department of Bioagricultural Sciences, National Chiayi University, No. 300 Syuefu Rd., Chiayi 60004, Taiwan, ROC.
authors.forename: Jing-Yu
authors.initials: JY
authors.lastname: Lee
authors.forename: I-Chun
authors.initials: IC
authors.lastname: Chien
authors.forename: Win-Yu
authors.initials: WY
authors.lastname: Lin
authors.forename: Shao-Min
authors.initials: SM
authors.lastname: Wu
authors.forename: Bo-Huei
authors.initials: BH
authors.lastname: Wei
authors.forename: Yu-En
authors.initials: YE
authors.lastname: Lee
authors.forename: Hu-Hui
authors.initials: HH
authors.lastname: Lee
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D002467:Cell Nucleus / Q000502:physiology; D055785:Gene Knockdown Techniques; D017930:Genes, Reporter; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D060588:LIM Domain Proteins / Q000235:genetics / Q000378:metabolism*; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D024510:Muscle Development; D018485:Muscle Fibers, Skeletal / Q000378:metabolism / Q000502:physiology; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism*; D018008:Myogenin / Q000235:genetics / Q000378:metabolism; D018995:Myosin Heavy Chains / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D034622:RNA Interference; D011994:Recombinant Proteins / Q000096:biosynthesis; D014158:Transcription, Genetic; D015854:Up-Regulation; D060449:Wnt Signaling Pathway; D051176:beta Catenin / Q000096:biosynthesis
chemical_list: C486306:Fhl1 protein, mouse; D047908:Intracellular Signaling Peptides and Proteins; D060588:LIM Domain Proteins; D009124:Muscle Proteins; C503801:Myog protein, mouse; D018008:Myogenin; D011994:Recombinant Proteins; D051176:beta Catenin; D008156:Luciferases; D018995:Myosin Heavy Chains