title: Increased expression of EMMPRIN and VEGF in the rat brain after gamma irradiation.
abstract: The extracellular matrix metalloproteinase inducer (EMMPRIN) has been known to play a key regulatory role in pathological angiogenesis. A elevated activation of vascular endothelial growth factor (VEGF) following radiation injury has been shown to mediate blood-brain barrier (BBB) breakdown. However, the roles of EMMPRIN and VEGF in radiation-induced brain injury after gamma knife surgery (GKS) are not clearly understood. In this study, we investigated EMMPRIN changes in a rat model of radiation injury following GKS and examined potential associations between EMMPRIN and VEGF expression. Adult male rats were subjected to cerebral radiation injury by GKS under anesthesia. We found that EMMPRIN and VEGF expression were markedly upregulated in the target area at 8-12 weeks after GKS compared with the control group by western blot, immunohistochemistry, and RT-PCR analysis. Immunofluorescent double staining demonstrated that EMMPRIN signals colocalized with caspase-3 and VEGF-positive cells. Our data also demonstrated that increased EMMPRIN expression was correlated with increased VEGF levels in a temporal manner. This is the first study to show that EMMPRIN and VEGF may play a role in radiation injuries of the central nervous system after GKS.
authors.affiliation: Department of Neurosurgery, the Second Hospital of Tianjin Medical University, Tianjin, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Wei
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.forename: Huiling
authors.initials: H
authors.lastname: Huang
authors.forename: Desheng
authors.initials: D
authors.lastname: Xu
authors.forename: Dashi
authors.initials: D
authors.lastname: Zhi
authors.forename: Dong
authors.initials: D
authors.lastname: Liu
authors.forename: Yipei
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D051926:Basigin / Q000378:metabolism*; D001921:Brain / Q000098:blood supply / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*; D001930:Brain Injuries / Q000378:metabolism / Q000473:pathology; D053148:Caspase 3 / Q000378:metabolism; D005720:Gamma Rays / Q000009:adverse effects*; D007150:Immunohistochemistry; D008297:Male; D046529:Microscopy, Electron, Transmission; D010296:Parietal Lobe / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects; D011833:Radiation Injuries, Experimental / Q000378:metabolism; D016634:Radiosurgery / Q000009:adverse effects; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: D042461:Vascular Endothelial Growth Factor A; C467498:vascular endothelial growth factor A, rat; D051926:Basigin; C505410:Casp3 protein, rat; D053148:Caspase 3