title: The potential role of ORM2 in the development of colorectal cancer.
abstract: Colorectal cancer (CRC) is the third most common malignancy in the world. The risk of death is closely correlated to the stage of CRC at the time of primary diagnosis. Therefore, there is a compelling need for the identification of blood biomarkers that can enable early detection of CRC. We used a quantitative proteomic approach with isobaric labeling (iTRAQ) to examine changes in the plasma proteome of 10 patients with CRC compared to healthy volunteers. Enzyme-Linked Immunosorbnent Assay (ELISA) and Western blot were used for further validation. In our quantitative proteomics analysis, we detected 75 human plasma proteins with more than 95% confidence using iTRAQ labeling in conjunction with microQ-TOF MS. 9 up-regulated and 4 down-regulated proteins were observed in the CRC group. The ORM2 level in plasma was confirmed to be significantly elevated in patients suffering from CRC compared with the controls. ORM2 expression in CRC tissues was significantly increased compared with that in corresponding adjacent normal mucous tissues (P<0.001). ITRAQ together with Q-TOF/MS is a sensitive and reproducible technique of quantitative proteomics. Alteration in expression of ORM2 suggests that ORM2 could be used as a potential biomarker in the diagnosis of CRC.
authors.affiliation: Department of Clinical Laboratory, Qilu Hospital, Shandong University, Jinan, Shandong, China.
authors.forename: Xuhua
authors.initials: X
authors.lastname: Zhang
authors.forename: Zhiying
authors.initials: Z
authors.lastname: Xiao
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Liu
authors.forename: Lutao
authors.initials: L
authors.lastname: Du
authors.forename: Lili
authors.initials: L
authors.lastname: Wang
authors.forename: Shun
authors.initials: S
authors.lastname: Wang
authors.forename: Ni
authors.initials: N
authors.lastname: Zheng
authors.forename: Guixi
authors.initials: G
authors.lastname: Zheng
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Zhaogang
authors.initials: Z
authors.lastname: Dong
authors.forename: Xuewei
authors.initials: X
authors.lastname: Zhuang
authors.forename: Chuanxin
authors.initials: C
authors.lastname: Wang
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism; D015153:Blotting, Western; D002471:Cell Transformation, Neoplastic / Q000378:metabolism* / Q000473:pathology*; D015179:Colorectal Neoplasms / Q000097:blood / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology; D008297:Male; D013058:Mass Spectrometry; D009363:Neoplasm Proteins / Q000097:blood / Q000378:metabolism*; D009961:Orosomucoid / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; C573095:ORM2 protein, human; D009961:Orosomucoid