title: Immunostaining of Ectocarpus cells.
abstract: This article describes an immunostaining protocol for Ectocarpus that was optimized for the detection of tubulin but could be used with any suitable antibody. Ectocarpus has small but relatively transparent cells and the uniseriate filaments can be grown directly attached to the surface of microscope slides. These features make Ectocarpus particularly suitable for high resolution imaging approaches, both in vivo or after fixation. All incubations described below are carried out on a platform shaker at room temperature. Use high-quality microscope slides to avoid imperfections in the glass that can be a problem for confocal laserscan microscopy analysis.
authors.affiliation: UPMC Université Paris 06, The Marine Plants and Biomolecules Laboratory, UMR 7139, Station Biologique de Roscoff, 29682 Roscoff Cedex, France.
authors.forename: Susana M
authors.initials: SM
authors.lastname: Coelho
authors.forename: Delphine
authors.initials: D
authors.lastname: Scornet
authors.forename: Sylvie
authors.initials: S
authors.lastname: Rousvoal
authors.forename: Nick
authors.initials: N
authors.lastname: Peters
authors.forename: Laurence
authors.initials: L
authors.lastname: Dartevelle
authors.forename: Akira F
authors.initials: AF
authors.lastname: Peters
authors.forename: J Mark
authors.initials: JM
authors.lastname: Cock
mesh_terms: D000199:Actins / Q000276:immunology; D000906:Antibodies / Q000276:immunology; D007150:Immunohistochemistry / Q000379:methods*; D000459:Phaeophyceae / Q000166:cytology*; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D000199:Actins; D000906:Antibodies