title: In vitro insulin release from thermosensitive chitosan hydrogel.
abstract: Recently, great attention has been paid to in situ gel-forming chitosan/glycerol-phosphate (chitosan/Gp) solution due to their good biodegradability and thermosensitivity. This in situ gel-forming system is injectable fluid that can be introduced into the body in a minimally invasive manner prior to solidifying within the desired tissue. At the present study, insulin release from chitosan/Gp solution has been investigated. Insulin in different concentrations was loaded in two formulations of chitosan/Gp solution and in vitro drug release was studied over a period of 3 weeks. Results indicated that the release of insulin from chitosan/Gp gel decreases by increasing in Gp salt and initial insulin concentration. Stability of released insulin was investigated by 8-anilino-1-naphthalenesulfonate probe. Results proved that insulin have been released in its native form. Because of simple preparation and administration, prolonged release of insulin and stability of released insulin, this in situ gel-forming system could be used as a controlled release delivery system for insulin.
authors.affiliation: Pharmaceutical Research Center and School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Elham
authors.initials: E
authors.lastname: Khodaverdi
authors.forename: Mohsen
authors.initials: M
authors.lastname: Tafaghodi
authors.forename: Fariba
authors.initials: F
authors.lastname: Ganji
authors.forename: Khalil
authors.initials: K
authors.lastname: Abnoos
authors.forename: Hanie
authors.initials: H
authors.lastname: Naghizadeh
mesh_terms: D002626:Chemistry, Pharmaceutical; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D004355:Drug Stability; D005994:Glycerophosphates / Q000737:chemistry; D020100:Hydrogels; D006863:Hydrogen-Ion Concentration; D007004:Hypoglycemic Agents / Q000737:chemistry*; D007328:Insulin / Q000737:chemistry*; D007700:Kinetics; D055550:Protein Stability; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D012995:Solubility; D013678:Technology, Pharmaceutical / Q000379:methods; D013696:Temperature; D014783:Viscosity
chemical_list: D003692:Delayed-Action Preparations; D004337:Drug Carriers; D005994:Glycerophosphates; D020100:Hydrogels; D007004:Hypoglycemic Agents; D007328:Insulin; C550160:N-(2-hydroxypropyl)-3-trimethylammonium chitosan; D000644:Quaternary Ammonium Compounds; D048271:Chitosan; C031463:beta-glycerophosphoric acid