title: [Evaluation on application of common diagnosis methods for schistosomiasis japonica in endemic areas of China. III. Analysis and evaluation of underestimation of prevalence of Schistosoma japonicum infection by routine Kato-Katz technique].
abstract: OBJECTIVE
  To understand the rates of underestimation of Schistosoma japonicum infection by Kato-Katz technique with different stool samples and different smears in field site.
  
  
  METHODS
  With 27 Kato-Katz thick smears (3 stool samples with 9 thick smears each) for 3 consecutive years as a "gold standard", the rates of underestimation of schistosome infection by Kato-Katz technique with different stool samples and different smears were evaluated in a schistosomiasis endemic village.
  
  
  RESULTS
  The examined rates of schistosome infection increased and the rates of underestimation decreased gradually with the increase of the number of Kato-Katz thick smears examined. The rates of underestimation of 3 smears were 40.98%-50.80% and the rates of underestimation of 6 smears were 25.48%-32.39%. The rates of schistosome infection based on the reading of 27 Kato-Katz thick smears were 10.96% in 2008, 8.54% in 2009 and 3.73% in 2010 respectively, but the rates of underestimation of various Kato-Katz thick smears were similar. There were no significant differences among the readings of 3, 6, and 9 smears of 1 stool sample, 3, 6 and 9 smears of 3 stool samples, 6 smears of 2 stool samples, 6 smears of 1 stool sample, and 6 smears of 3 stool samples.
  
  
  CONCLUSIONS
  In a community with low-intensity of schistosome infection, the rate of underestimation is big by Kato-Katz technique with the reading of 3 smears of 1 stool sample. It suggested that it is better to increase smears rather than the number of stool samples examined.
authors.affiliation: Jiangxi Provincial Institute of Parasitic Diseases, Nanchang 330046, China. jxlindandan@163.com
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Lin
authors.forename: Yue-Min
authors.initials: YM
authors.lastname: Liu
authors.forename: Fei
authors.initials: F
authors.lastname: Hu
authors.forename: Yi-Feng
authors.initials: YF
authors.lastname: Li
authors.forename: Bo
authors.initials: B
authors.lastname: Tao
authors.forename: Min
authors.initials: M
authors.lastname: Yuan
authors.forename: Shu-Ying
authors.initials: SY
authors.lastname: Xie
authors.forename: Mei-Jiao
authors.initials: MJ
authors.lastname: Huang
authors.forename: Qiu-Lin
authors.initials: QL
authors.lastname: Jiang
authors.forename: Jian-Ying
authors.initials: JY
authors.lastname: Li
authors.forename: Zu-Lu
authors.initials: ZL
authors.lastname: Gao
authors.forename: Jin-Ming
authors.initials: JM
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D002648:Child; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D019937:Diagnostic Techniques and Procedures; D019353:Endemic Diseases; D005243:Feces / Q000469:parasitology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D015995:Prevalence; D012549:Schistosoma japonicum / Q000302:isolation & purification* / Q000502:physiology; D012554:Schistosomiasis japonica / Q000175:diagnosis* / Q000453:epidemiology / Q000469:parasitology; D055815:Young Adult