title: Estimation of the subunit stoichiometry of the membrane-associated daptomycin oligomer by FRET.
abstract: Daptomycin is a lipopeptide antibiotic that kills Gram-positive bacteria by depolarizing their cell membranes. This antibacterial action of daptomycin is correlated with the formation of membrane-associated oligomers. We here examine the number of subunits contained in one oligomer using fluorescence resonance energy transfer (FRET). The results suggest that the oligomer contains approximately 6 to 7 subunits, or possibly twice this number if it spans both membrane monolayers.
authors.affiliation: Department of Chemistry, University of Waterloo, Waterloo, Ontario, Canada.
authors.forename: Jawad K
authors.initials: JK
authors.lastname: Muraih
authors.forename: Michael
authors.initials: M
authors.lastname: Palmer
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D002462:Cell Membrane / Q000737:chemistry* / Q000187:drug effects; D017576:Daptomycin / Q000737:chemistry* / Q000494:pharmacology; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D006094:Gram-Positive Bacteria / Q000187:drug effects; D007700:Kinetics; D007737:Kynurenine / Q000737:chemistry; D055666:Lipopeptides / Q000737:chemistry / Q000494:pharmacology; D008956:Models, Chemical; D008958:Models, Molecular; D055503:Protein Multimerization; D021122:Protein Subunits / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D055666:Lipopeptides; D021122:Protein Subunits; D007737:Kynurenine; D017576:Daptomycin