title: Rickettsia slovaca and Rickettsia raoultii in Dermacentor marginatus and Dermacentor reticulatus ticks from Slovak Republic.
abstract: Rickettsiae, obligate intracellular Gram-negative bacteria, responsible for mild to severe diseases in humans are associated with arthropod vectors. Dermacentor marginatus and Dermacentor reticulatus are known vectors of Rickettsia slovaca and Rickettsia raoultii distributed across Europe. A total of 794 D. marginatus, D. reticulatus and Ixodes ricinus adult ticks were collected from the vegetation, removed from horses, sheep, goats and dogs in Slovakia. The DNA of Rickettsia sp. was found in 229 ticks by PCR amplifying parts of gltA, ompA and sca4 genes. Next analyses of Rickettsia-positive samples by PCR-RFLP and/or sequencing showed D. reticulatus ticks were more infected with R. raoultii and D. marginatus were more infected with R. slovaca. The prevalence of R. raoultii was 8.1-8.6% and 22.3-27% in D. marginatus and D. reticulatus, respectively. The prevalence of R. slovaca was 20.6-24.3% in D. marginatus and 1.7-3.4% in D. reticulatus. Intracellular growth of R. raoultii isolate from D. marginatus tick was evaluated by rOmpA-based quantitative SybrGreen PCR assay. The highest point of multiplication was recorded on the 7th and 8th day postinfection in Vero and L929 cells, respectively. R. raoultii was transmitted during feeding of R. raoultii-positive ticks to guinea pigs and subsequently rickettsial infection was recorded in all organs, the highest infection was in spleen, liver and heart. Our study describes the detection and isolation of tick-borne pathogens R. raoultii and R. slovaca, show that they are spread in Slovakia and highlight their risk for humans.
authors.affiliation: Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovak Republic. eva.spitalska@savba.sk
authors.forename: Eva
authors.initials: E
authors.lastname: Spitalská
authors.forename: Katarína
authors.initials: K
authors.lastname: Stefanidesová
authors.forename: Elena
authors.initials: E
authors.lastname: Kocianová
authors.forename: Vojtech
authors.initials: V
authors.lastname: Boldiš
mesh_terms: D000818:Animals; D002460:Cell Line; D004269:DNA, Bacterial / Q000145:classification / Q000235:genetics; D003870:Dermacentor / Q000382:microbiology*; D016701:Guinea; D006168:Guinea Pigs; D006801:Humans; D010802:Phylogeny; D012281:Rickettsia / Q000302:isolation & purification*; D012282:Rickettsia Infections / Q000453:epidemiology / Q000382:microbiology* / Q000635:transmission; D018154:Slovakia; D013997:Time Factors
chemical_list: D004269:DNA, Bacterial