title: Isolation of natural products by low-pressure column chromatography.
abstract: This chapter describes the isolation of natural products using low-pressure column chromatography (LPLC) and provides a brief summary of the adsorption and size exclusion processes involved in LPLC. Different types of stationary phases used in both adsorption and size exclusion LPLC are also presented with specific examples. Various operational parameters in relation to column packing (both wet and dry), column equilibration, sample application, and column development, and a summary of generic procedures for adsorption and size exclusion LPLC are also incorporated in this chapter. Throughout the chapter, several specific examples of the application of LPLC for the isolation of various natural products including alkaloids, coumarins, flavones, and saponins from various plants are given. Many of these examples include a combination of adsorption and size exclusion chromatography to obtain specific compounds.
authors.affiliation: School of Pharmacy, The Robert Gordon University, Aberdeen, UK. R.Reid@rgu.ac.uk
authors.forename: Raymond G
authors.initials: RG
authors.lastname: Reid
authors.forename: Satyajit D
authors.initials: SD
authors.lastname: Sarker
mesh_terms: D000327:Adsorption; D000470:Alkaloids / Q000302:isolation & purification; D000537:Aluminum Oxide / Q000737:chemistry; D001688:Biological Products / Q000302:isolation & purification; D002850:Chromatography, Gel / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods; D056148:Chromatography, Reverse-Phase / Q000379:methods; D005663:Furans / Q000302:isolation & purification; D007004:Hypoglycemic Agents / Q000302:isolation & purification; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000302:isolation & purification*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification; D011137:Polystyrenes / Q000737:chemistry; D012717:Sesquiterpenes / Q000302:isolation & purification; D058428:Silica Gel / Q000737:chemistry; D012997:Solvents / Q000737:chemistry
chemical_list: D000470:Alkaloids; D001688:Biological Products; D005663:Furans; D007004:Hypoglycemic Agents; C545396:LSP1 polysaccharide; C545395:LSP2 polysaccharide; D010936:Plant Extracts; D011134:Polysaccharides; D011137:Polystyrenes; D012717:Sesquiterpenes; D012997:Solvents; D058428:Silica Gel; D000537:Aluminum Oxide