title: Effects of preeclampsia on the yield of hematopoietic stem cells obtained from umbilical cord blood at delivery.
abstract: OBJECTIVE
  To compare the numbers of cord blood CD34(+) hematopoietic stem cells (HSC) between preeclampsia (PE) and control (non-PE) subjects and to determine the factors that may influence this observation.
  
  
  METHODS
  Umbilical cord blood was collected from 28 PE and 19 non-PE subjects. Nucleated and CD34(+) cell counts were derived using the Trucount tube-based stem cell enumeration kit on BD FACSCalibur.
  
  
  RESULTS
  The cord blood volume, nucleated and CD34(+) cell counts were significantly reduced in PE subjects compared to non-PE subjects. Among the PE subjects, systolic and diastolic blood pressure demonstrated a negative correlation with total nucleated and CD34(+) cell counts. Gestational age at delivery influenced cord blood volume and nucleated cell counts, but not CD34(+) cell counts. Birth weight and placental weight correlated strongly with cord blood volume, and nucleated and CD34(+) cell counts. There were no correlations observed between cord blood parameters and maternal age, maternal white cell count, gravidity, route of delivery or neonatal gender among PE subjects.
  
  
  CONCLUSIONS
  Preeclampsia has a negative impact on the yield of HSC obtained from cord blood at delivery. Maternal blood pressure, neonatal birth weight and placental weight are important factors influencing the numbers of cord blood HSC. These findings should be taken into consideration when selecting cord blood units from mothers with PE for banking. Selecting the heaviest term neonate might improve the yield of cord blood HSC obtained from PE mothers.
authors.affiliation: Cell Therapy Centre, Department of Medicine, Universiti Kebangsaan Malaysia Medical Centre, Kuala Lumpur, Malaysia. sfadilah@ppukm.ukm.my
authors.forename: Fadilah S Abdul
authors.initials: FS
authors.lastname: Wahid
authors.forename: Mona Zaria
authors.initials: MZ
authors.lastname: Nasaruddin
authors.forename: Mohd Razif Mohd
authors.initials: MR
authors.lastname: Idris
authors.forename: Maiza
authors.initials: M
authors.lastname: Tusimin
authors.forename: Nor Rafeah
authors.initials: NR
authors.lastname: Tumian
authors.forename: Zaleha Abdullah
authors.initials: ZA
authors.lastname: Mahdy
mesh_terms: D000328:Adult; D018952:Antigens, CD34 / Q000378:metabolism; D016022:Case-Control Studies; D002452:Cell Count; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D006412:Hematopoietic Stem Cells / Q000378:metabolism; D006801:Humans; D011225:Pre-Eclampsia / Q000097:blood*; D011247:Pregnancy; D011446:Prospective Studies
chemical_list: D018952:Antigens, CD34