title: Development of an ELISA-array for simultaneous detection of five encephalitis viruses.
abstract: Japanese encephalitis virus(JEV), tick-borne encephalitis virus(TBEV), and eastern equine encephalitis virus (EEEV) can cause symptoms of encephalitis. Establishment of accurate and easy methods by which to detect these viruses is essential for the prevention and treatment of associated infectious diseases. Currently, there are still no multiple antigen detection methods available clinically. An ELISA-array, which detects multiple antigens, is easy to handle, and inexpensive, has enormous potential in pathogen detection. An ELISA-array method for the simultaneous detection of five encephalitis viruses was developed in this study. Seven monoclonal antibodies against five encephalitis-associated viruses were prepared and used for development of the ELISA-array. The ELISA-array assay is based on a "sandwich" ELISA format and consists of viral antibodies printed directly on 96-well microtiter plates, allowing for direct detection of 5 viruses. The developed ELISA-array proved to have similar specificity and higher sensitivity compared with the conventional ELISAs. This method was validated by different viral cultures and three chicken eggs inoculated with infected patient serum. The results demonstrated that the developed ELISA-array is sensitive and easy to use, which would have potential for clinical use.
authors.affiliation: Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Kang
authors.forename: Yuchang
authors.initials: Y
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Fan
authors.forename: Fang
authors.initials: F
authors.lastname: Lin
authors.forename: Jingjing
authors.initials: J
authors.lastname: Wei
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Zhu
authors.forename: Yi
authors.initials: Y
authors.lastname: Hu
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Guohui
authors.initials: G
authors.lastname: Chang
authors.forename: Qingyu
authors.initials: Q
authors.lastname: Zhu
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Yinhui
authors.initials: Y
authors.lastname: Yang
mesh_terms: D000818:Animals; D000914:Antibodies, Viral; D002645:Chickens; D019411:Clinical Laboratory Techniques / Q000379:methods*; D004663:Encephalitis Virus, Eastern Equine / Q000302:isolation & purification*; D004664:Encephalitis Virus, Japanese / Q000302:isolation & purification*; D004669:Encephalitis Viruses, Tick-Borne / Q000302:isolation & purification*; D018792:Encephalitis, Viral / Q000175:diagnosis* / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods*
chemical_list: D000914:Antibodies, Viral