title: Impaired cutaneous wound healing in transforming growth factor-β inducible early gene1 knockout mice.
abstract: Transforming growth factor-β inducible early gene (TIEG) is induced by transforming growth factor-β (TGF-β) and acts as the primary response gene in the TGF-β/Smad pathway. TGF-β is a multifunctional growth factor that affects dermal wound healing; however, the mechanism of how TGF-β affects wound healing is still not well understood because of the complexity of its function and signaling pathways. We hypothesize that TIEG may play a role in dermal wound healing, with involvement in wound closure, contraction, and reepithelialization. In this study, we have shown that TIEG1 knockout (TIEG1-/-) mice have a delay in wound closure related to an impairment in wound contraction, granulation tissue formation, collagen synthesis, and reepithelialization. We also found that Smad7 was increased in the wounds and appeared to play a role in this wound healing model in TIEG1-/- mice.
authors.affiliation: Division of Plastic and Reconstructive Surgery and Critical Care, Department of Surgery, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Keijiro
authors.initials: K
authors.lastname: Hori
authors.forename: Jie
authors.initials: J
authors.lastname: Ding
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Marcoux
authors.forename: Takashi
authors.initials: T
authors.lastname: Iwashina
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sakurai
authors.forename: Edward E
authors.initials: EE
authors.lastname: Tredget
mesh_terms: D000818:Animals; D016207:Cytokines / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D006097:Granulation Tissue / Q000378:metabolism / Q000473:pathology*; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D060888:Real-Time Polymerase Chain Reaction; D012867:Skin / Q000293:injuries / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D051785:Smad Proteins / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D015854:Up-Regulation; D014945:Wound Healing; D051741:Kruppel-Like Transcription Factors; D051765:Early Growth Response Transcription Factors
chemical_list: D016207:Cytokines; D004268:DNA-Binding Proteins; D051785:Smad Proteins; D014157:Transcription Factors; D016212:Transforming Growth Factor beta; C569958:KLF10 protein, mouse; D051741:Kruppel-Like Transcription Factors; D051765:Early Growth Response Transcription Factors