title: Inhibition of transient receptor potential melastain 7 channel increases HSCs apoptosis induced by TRAIL.
abstract: OBJECTIVE
  Transient receptor potential melastain 7 (TRPM7) channels are known to have a fundamental role in many cellular processes and pathology of various diseases. The objective of this study was to investigate the potential relationship between TRPM7 and the apoptosis of hepatic stellate cells (HSCs) induced by TNF-related apoptosis inducing-ligand (TRAIL).
  
  
  METHODS
  In this study, using a combination of Western blotting, RT-PCR and flow cytometric analysis, we investigated the influence and potential function of TRPM7 channels on the apoptosis induced by TRAIL in HSCs which is the key cell of formation of extracellular matrix (ECM) and is also the core link of occurrence of hepatic fibrosis (HF).
  
  
  RESULTS
  We observed significant expression of TRPM7 mRNA and protein in HSCs. Suppression of TRPM7 channels by 2-aminoethoxydiphenyl borate (2-APB) or Gd3+ not only markedly eliminated TRPM7 expression, but also increased the apoptosis of HSCs induced by TRAIL, a major apoptosis stimulator of HSCs.
  
  
  CONCLUSIONS
  Our findings strongly suggest that TRPM7 channels are involved in the apoptosis of HSCs induced by TRAIL, probably by regulating the sensitivity of HSCs to TRAIL.
authors.affiliation: School of Pharmacy, Anhui Medical University, Hefei, Anhui 230032, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Huang
authors.forename: Cheng
authors.initials: C
authors.lastname: Huang
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D001896:Boron Compounds / Q000494:pharmacology; D053148:Caspase 3 / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D005109:Extracellular Matrix / Q000378:metabolism*; D005434:Flow Cytometry; D005682:Gadolinium / Q000494:pharmacology; D055166:Hepatic Stellate Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D008103:Liver Cirrhosis / Q000378:metabolism*; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000378:metabolism*; D050053:TRPM Cation Channels / Q000037:antagonists & inhibitors* / Q000378:metabolism; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D001896:Boron Compounds; D017931:DNA Primers; D053221:TNF-Related Apoptosis-Inducing Ligand; D050053:TRPM Cation Channels; D013778:Tetrazolium Salts; D013844:Thiazoles; C505655:Tnfsf10 protein, rat; D005682:Gadolinium; C109986:2-aminoethoxydiphenyl borate; C486584:Trpm7 protein, rat; D053148:Caspase 3; C022616:thiazolyl blue