title: Influenza viruses expressing chimeric hemagglutinins: globular head and stalk domains derived from different subtypes.
abstract: The influenza virus hemagglutinin molecule possesses a globular head domain that mediates receptor binding and a stalk domain at the membrane-proximal region. We generated functional influenza viruses expressing chimeric hemagglutinins encompassing a variety of globular head and stalk combinations, not only from different hemagglutinin subtypes but also from different hemagglutinin phylogenetic groups. These chimeric recombinant viruses possess growth properties similar to those of wild-type influenza viruses and can be used as reagents to measure domain-specific antibodies in virological and immunological assays.
authors.affiliation: Department of Microbiology, Global Health and Emerging Pathogens Institute, Mount Sinai School of Medicine, New York, New York, USA.
authors.forename: Rong
authors.initials: R
authors.lastname: Hai
authors.forename: Florian
authors.initials: F
authors.lastname: Krammer
authors.forename: Gene S
authors.initials: GS
authors.lastname: Tan
authors.forename: Natalie
authors.initials: N
authors.lastname: Pica
authors.forename: Dirk
authors.initials: D
authors.lastname: Eggink
authors.forename: Jad
authors.initials: J
authors.lastname: Maamary
authors.forename: Irina
authors.initials: I
authors.lastname: Margine
authors.forename: Randy A
authors.initials: RA
authors.lastname: Albrecht
authors.forename: Peter
authors.initials: P
authors.lastname: Palese
mesh_terms: D000914:Antibodies, Viral / Q000276:immunology; D002460:Cell Line; D002678:Chimera / Q000145:classification / Q000235:genetics / Q000276:immunology; D015870:Gene Expression; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000737:chemistry* / Q000235:genetics* / Q000276:immunology; D006801:Humans; D009980:Influenza A virus / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000276:immunology; D007251:Influenza, Human / Q000276:immunology / Q000821:virology*; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D011995:Recombination, Genetic
chemical_list: D000914:Antibodies, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus