title: Effects of components of semen extenders on the binding of stallion spermatozoa to bovine or equine zonae pellucidae.
abstract: The effects of semen extender components on the ability of stallion sperm to bind to the zona pellucida (ZP) and the suitability of using bovine ZP for a ZP-binding assay for stallion sperm were investigated in a series of experiments. In Experiment I, binding of stallion sperm to both bovine and equine ZP was significantly increased when a skim milk-based extender (EZM) was used. In Experiment II, a threefold increase in sperm binding to ZP was observed when sperm were diluted in EZM compared with diluents, which contained no milk (TALP, LAC, and EmCare). In Experiment III, centrifuging the sperm through Percoll did not increase sperm binding to the ZP but did remove any positive effect of EZM on sperm-ZP binding. In Experiment IV, exposure of either sperm or ZP to EZM before co-incubation did not increase sperm binding to ZP. In Experiment V, sperm diluted in TALP containing skim milk, EZM, or INRA96 bound more efficiently to the ZP than sperm diluted in TALP without milk proteins. In Experiment VI, sodium caseinate, native phosphocaseinate, and caseinoglycopeptide increased sperm binding to the ZP. In conclusion, diluents containing milk or milk proteins markedly enhanced the number of sperm bound to both equine and bovine ZP.
authors.affiliation: Department of Veterinary Clinical Sciences, The Ohio State University, Columbus, Ohio 43210, USA. marco.dasilva@cvm.osu.edu
authors.forename: Marco A
authors.initials: MA
authors.lastname: Coutinho da Silva
authors.forename: George E
authors.initials: GE
authors.lastname: Seidel
authors.forename: Edward L
authors.initials: EL
authors.lastname: Squires
authors.forename: James K
authors.initials: JK
authors.lastname: Graham
authors.forename: Elaine M
authors.initials: EM
authors.lastname: Carnevale
mesh_terms: D000818:Animals; D002364:Caseins / Q000494:pharmacology; D002417:Cattle; D002498:Centrifugation; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary; D006736:Horses; D008297:Male; D008894:Milk Proteins / Q000494:pharmacology*; D010446:Peptide Fragments / Q000494:pharmacology; D011205:Povidone / Q000494:pharmacology; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D012822:Silicon Dioxide / Q000494:pharmacology; D013084:Sperm-Ovum Interactions / Q000187:drug effects*; D013090:Spermatocytes / Q000187:drug effects*; D015044:Zona Pellucida / Q000187:drug effects*
chemical_list: D002364:Caseins; D008894:Milk Proteins; D010446:Peptide Fragments; C065989:caseinomacropeptide; C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone