title: High level population differentiation of finless porpoises (Neophocaena phocaenoides) in Chinese waters revealed by sequence variability of four nuclear introns.
abstract: In the present study, sequence variations at four nuclear introns which were respectively from the parathyroid hormone-like (PTH) gene, isolate Pdalz1692 interferon (IFN) gene, peripherin-like (RDS) gene, and tyrosine kinase receptor-like (KIT) gene, were examined to analyze genetic diversity and population structure of the finless porpoise (Neophocaena phocaenoides) in Chinese waters. High among-population differentiation was revealed, with a significant genetic structure between populations (PTH: F(ST) = 0.29, P < 0.001; IFN1@: F(ST) = 0.23, P < 0.001; RDS: F(ST) = 0.12, P < 0.001; KIT: F(ST) = 0.16, P < 0.001) shown by the analysis of molecular variance. Although common haplotypes accounted for more than one half of all samples examined, many haplotypes were found to be population-specific. The Tajima's D, Fu's tests and mismatch distributions all suggested a recent colonization and population expansion of finless porpoises in Chinese waters. In view of special reference to the conservation priority of the Yangtze finless porpoises, special protection measures must be taken urgently for this population.
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210046, China. jujianfeng@njnu.edu.cn
authors.forename: Jianfeg
authors.initials: J
authors.lastname: Ju
authors.forename: Mei
authors.initials: M
authors.lastname: Yang
authors.forename: Shixia
authors.initials: S
authors.lastname: Xu
authors.forename: Kaiya
authors.initials: K
authors.lastname: Zhou
authors.forename: Guang
authors.initials: G
authors.lastname: Yang
mesh_terms: D000818:Animals; D001483:Base Sequence; D002681:China; D014644:Genetic Variation; D007372:Interferons / Q000235:genetics*; D007381:Intermediate Filament Proteins / Q000235:genetics*; D007438:Introns; D008562:Membrane Glycoproteins / Q000235:genetics*; D009419:Nerve Tissue Proteins / Q000235:genetics*; D010281:Parathyroid Hormone / Q000235:genetics*; D064531:Peripherins; D058974:Phylogeography; D019576:Porpoises / Q000235:genetics*; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D007381:Intermediate Filament Proteins; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D010281:Parathyroid Hormone; D064531:Peripherins; D007372:Interferons; D011505:Protein-Tyrosine Kinases