title: Exocyst subunits are involved in isoproterenol-induced amylase release from rat parotid acinar cells.
abstract: Exocytosis of secretory granules in parotid acinar cells requires multiple events: tethering, docking, priming, and fusion with a luminal plasma membrane. The exocyst complex, which is composed of eight subunits (Sec3, Sec5, Sec6, Sec8, Sec10, Sec15, Exo70, and Exo84) that are conserved in yeast and mammalian cells, is thought to participate in the exocytotic pathway. However, to date, no exocyst subunit has been identified in salivary glands. In the present study, we investigated the expression and function of exocyst subunits in rat parotid acinar cells. The expression of mRNA for all eight exocyst subunits was detected in parotid acinar cells by RT-PCR, and Sec6 and Sec8 proteins were localized on the luminal plasma membrane. Sec6 interacted with Sec8 after 5 min of stimulation with isoproterenol. In addition, antibodies to-Sec6 and Sec8 inhibited isoproterenol-induced amylase release from streptolysin O-permeabilized parotid acinar cells. These results suggest that an exocyst complex of eight subunits is required for amylase release from parotid acinar cells.
authors.affiliation: Department of Biochemistry, School of Life Dentistry at Niigata, The Nippon Dental University, Chuo-ku, Niigata, Japan. imaiak@ngt.ndu.ac.jp
authors.forename: Akane
authors.initials: A
authors.lastname: Imai
authors.forename: Sumio
authors.initials: S
authors.lastname: Yoshie
authors.forename: Maiko
authors.initials: M
authors.lastname: Haga-Tsujimura
authors.forename: Tomoko
authors.initials: T
authors.lastname: Nashida
authors.forename: Hiromi
authors.initials: H
authors.lastname: Shimomura
mesh_terms: D061354:Acinar Cells / Q000187:drug effects / Q000201:enzymology*; D000681:Amylases / Q000378:metabolism*; D000818:Animals; D002458:Cell Fractionation; D005089:Exocytosis / Q000502:physiology; D007545:Isoproterenol / Q000494:pharmacology*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000502:physiology*; D010306:Parotid Gland / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D051381:Rats; D013566:Sympathomimetics / Q000494:pharmacology*
chemical_list: D026901:Membrane Transport Proteins; D013566:Sympathomimetics; D000681:Amylases; D007545:Isoproterenol