title: Interleukin-8 associates with adhesion, migration, invasion and chemosensitivity of human gastric cancer cells.
abstract: OBJECTIVE
  To investigate the relationship between Interleukin-8 (IL-8) and proliferation, adhesion, migration, invasion and chemosensitivity of gastric cancer (GC) cells.
  
  
  METHODS
  The IL-8 cDNA was stably transfected into human GC cell line MKN-45 and selected IL-8-secreting transfectants. The expression of IL-8 in human GC cell line KATO-III was inhibited by RNA interference. The expressions of mRNA and protein of IL-8 in GC cells were detected by real-time reverse transcription-polymerase chain reaction or enzyme-linked immunosorbent assay (ELISA).
  
  
  RESULTS
  The overexpression of IL-8 resulted in an increased cell adhesion, migration and invasion, and a significant resistance to oxaliplatin in MKN-45 cells. Inhibition of IL-8 expression with small interfering RNA decreased the adhesion, migration and invasion functions and oxaliplatin resistance in KATO-III cells. IL-8 increased NF-κB and Akt activities and adhesion molecules ICAM-1, VCAM-1, and CD44 expression in GC cells.
  
  
  CONCLUSIONS
  Overexpression of IL-8 promotes the adhesion, migration, invasion, and chemoresistance of GC cells, indicating that IL-8 is an important therapeutic target in GC.
authors.affiliation: Department of Pediatrics, Huaian First Hospital Affiliated to Nanjing Medical University, Huaian 223300, Jiangsu Province, China.
authors.forename: Wen-Xia
authors.initials: WX
authors.lastname: Kuai
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wang
authors.forename: Xiao-Zhong
authors.initials: XZ
authors.lastname: Yang
authors.forename: Yao
authors.initials: Y
authors.lastname: Zhao
authors.forename: Ren
authors.initials: R
authors.lastname: Yu
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Tang
mesh_terms: D000970:Antineoplastic Agents / Q000627:therapeutic use; D002448:Cell Adhesion / Q000502:physiology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology*; D049109:Cell Proliferation; D019008:Drug Resistance, Neoplasm; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness / Q000473:pathology / Q000503:physiopathology; D009944:Organoplatinum Compounds / Q000627:therapeutic use; D000077150:Oxaliplatin; D034622:RNA Interference; D013274:Stomach Neoplasms / Q000188:drug therapy / Q000473:pathology / Q000503:physiopathology
chemical_list: D000970:Antineoplastic Agents; D016209:Interleukin-8; D009944:Organoplatinum Compounds; D000077150:Oxaliplatin