title: Myoglobin-directed assemblies of binary monolayers functionalized with iminodiacetic acid ligands at the air-water interface through metal coordination for multivalent protein binding.
abstract: Myoglobin binding to the binary monolayers composed of sodium hexadecylimino diacetate and hexadecanol at the air-water interface by means of metal coordination has been investigated using infrared reflection absorption spectroscopy (IRRAS). In the absence of Cu(2+), no myoglobin binding to the binary monolayers was observed. In the presence of Cu(2+), remarkable myoglobin binding to the binary monolayers resulted from the formation of ternary complexes of iminodiacetate (IDA)-Cu(2+)-surface histidine. Myoglobin-directed assemblies of the binary monolayers facilitated multivalent protein binding through lateral rearrangements of the IDA ligands and reorientations of the alkyl chains for enhanced protein binding. Myoglobin binding to and desorption from the binary monolayers could be readily controlled through metal coordination.
authors.affiliation: Key Laboratory of Mesoscopic Chemistry (Ministry of Education), and School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, PR China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Wang
authors.forename: Xuan
authors.initials: X
authors.lastname: Huang
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Xin
authors.forename: Xuezhong
authors.initials: X
authors.lastname: Du
mesh_terms: D000388:Air; D001665:Binding Sites; D003300:Copper / Q000737:chemistry*; D003998:Dicarboxylic Acids / Q000737:chemistry*; D005233:Fatty Alcohols / Q000737:chemistry*; D007098:Imino Acids / Q000737:chemistry*; D008024:Ligands; D008567:Membranes, Artificial; D009211:Myoglobin / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D003998:Dicarboxylic Acids; D005233:Fatty Alcohols; D007098:Imino Acids; D008024:Ligands; D008567:Membranes, Artificial; D009211:Myoglobin; C476508:sodium hexadecylimino diacetate; D014867:Water; D003300:Copper; C005031:cetyl alcohol; C008109:iminodiacetic acid