title: Structural insights into the classification of Mud Crab Reovirus.
abstract: Cryo-electron microscopy was applied to analyze mud crab reovirus (MCRV), which causes 'sleeping disease' in mud crab, Scylla serrata, a marine species cultured in China. We present here the three dimensional structure of MCRV at 13.8Å resolution. The outer capsid shell is composed of 260 trimers with complete T=13 icosahedral symmetry. A major difference between MCRV and previously reported aquareoviruses is that it lacks a pentameric turret structure. These results together with recently published molecular biological evidence (Deng et al., 2012) indicate that, from a structural perspective, MCRV should be classified as a new member of the family Reoviridae.
authors.affiliation: State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, People's Republic of China.
authors.forename: Zengwei
authors.initials: Z
authors.lastname: Huang
authors.forename: Xiexiong
authors.initials: X
authors.lastname: Deng
authors.forename: Yinyin
authors.initials: Y
authors.lastname: Li
authors.forename: Hongjun
authors.initials: H
authors.lastname: Su
authors.forename: Kunpeng
authors.initials: K
authors.lastname: Li
authors.forename: Zhixun
authors.initials: Z
authors.lastname: Guo
authors.forename: Peirui
authors.initials: P
authors.lastname: Zheng
authors.forename: Haidong
authors.initials: H
authors.lastname: Xu
authors.forename: Jianguo
authors.initials: J
authors.lastname: He
authors.forename: Qinfen
authors.initials: Q
authors.lastname: Zhang
authors.forename: Shaoping
authors.initials: S
authors.lastname: Weng
mesh_terms: D000818:Animals; D003386:Brachyura / Q000821:virology*; D002681:China; D002965:Classification; D020285:Cryoelectron Microscopy; D012087:Reoviridae / Q000145:classification* / Q000302:isolation & purification / Q000648:ultrastructure*; D014771:Virion / Q000648:ultrastructure*