title: (1)H, (13)C and (15)N resonance assignments of the periplasmic signalling domain of HasR, a TonB-dependent outer membrane heme transporter.
abstract: TonB-dependent transporters (TBDTs) are bacterial outer membrane proteins that internalize nutrients such as vitamin B12, metal complexes, heme, some carbohydrates, etc. In addition to their transport activity, several TBDTs are also involved in a signalling cascade from the cell surface into the cytoplasm, via their periplasmic signalling domain. Here we report the backbone and side chain resonance assignments of the signalling domain of HasR, a TonB-dependent outer membrane heme transporter from Serratia marcescens as a first step towards its structural study.
authors.affiliation: Institut Pasteur, Unité de RMN des Biomolécules, Département de Biologie Structurale et Chimie, 28, rue du Dr Roux, 75724, Paris Cedex 15, France.
authors.forename: Idir
authors.initials: I
authors.lastname: Malki
authors.forename: Gisele
authors.initials: G
authors.lastname: Cardoso de Amorim
authors.forename: Catherine
authors.initials: C
authors.lastname: Simenel
authors.forename: Ada
authors.initials: A
authors.lastname: Prochnicka-Chalufour
authors.forename: Muriel
authors.initials: M
authors.lastname: Delepierre
authors.forename: Nadia
authors.initials: N
authors.lastname: Izadi-Pruneyre
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000737:chemistry* / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular; D019897:Periplasm / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D012706:Serratia marcescens / Q000166:cytology
chemical_list: D001426:Bacterial Proteins; D026901:Membrane Transport Proteins