title: Comparative proteomic analysis in left ventricular remodeling following myocardial infarction in rats.
abstract: OBJECTIVE
  Left ventricular remodeling (LVR) following myocardial infarction (MI) is a key pathophysiological process in which MI develops into heart failure. The exact mechanism of LVR remains unclear. We performed differential proteomic analysis on the myocardia of rats with LVR after MI, to explore the mechanism of ventricular remodeling after MI.
  
  
  METHODS
  In the LVR group (n = 12), after the anterior descending coronary artery was ligated, the rats were fed for four weeks before the LVR models were established. Rats in the sham-operated group (n = 11) underwent thread-drawing without ligation. The hemodynamic parameters, pathological findings, and proteomics were compared between the two groups.
  
  
  RESULTS
  In the LVR group, the left ventricular end-diastolic pressure increased, the maximal left ventricular pressure increase/decrease ratio decreased significantly, and the left ventricular systolic pressure decreased. H-E staining and Masson staining of cardiac muscle tissues of the LVR group showed myocytolysis, disarray, and collagen proliferation. Twenty-one differentially expressed proteins were detected by proteomic analysis. We validated two proteins using western blot analysis. The differentially expressed proteins could be divided into six categories: energy metabolism-related proteins, cytoskeletal proteins, protein synthesis-related proteins, channel proteins, anti-oxidation- related proteins, and immune-related proteins.
  
  
  CONCLUSIONS
  These differentially expressed proteins might play key roles in LVR following MI.
authors.affiliation: China-Japan Union Hospital of Jilin University, Changchun, Jilin, China. zhouqiwei777@sina.com
authors.forename: Hong Juan
authors.initials: HJ
authors.lastname: Gu
authors.forename: Chang Bin
authors.initials: CB
authors.lastname: Gao
authors.forename: Jun Li
authors.initials: JL
authors.lastname: Gong
authors.forename: Xiang Jun
authors.initials: XJ
authors.lastname: Li
authors.forename: Bo
authors.initials: B
authors.lastname: Sun
authors.forename: Xi Ning
authors.initials: XN
authors.lastname: Li
mesh_terms: D000818:Animals; D008297:Male; D009203:Myocardial Infarction / Q000150:complications* / Q000473:pathology; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D020543:Proteome / Q000032:analysis; D040901:Proteomics; D051381:Rats; D017208:Rats, Wistar; D020257:Ventricular Remodeling
chemical_list: D020543:Proteome