title: Biological effects, total phenolic content and flavonoid concentrations of fragrant yellow onion (Allium flavum L.).
abstract: The antioxidant, antibacterial and antiproliferative activities, total phenolic content and concentrations of flavonoids of A. flavum extracts were determined. The total phenolic content was determined with Folin-Ciocalteu reagent and it ranged between 42.29 to 80.92 mg GA/g. The concentration of flavonoids in various extracts of A. flavum was determined using spectrophotometric method with aluminum chloride and obtained results varied from 64.07 to 95.71 mg RU/g. The antioxidant activity was monitored spectrophotometrically and expressed in terms of IC50 (μg/ml), and its values ranged from 64.34 to 243.34 μg/ml. The highest phenolic content and capacity to neutralize DPPH radicals were found in acetone extract. Antibacterial efficacy was defined by determining minimum inhibitory and minimum bactericidal concentrations using microdilution method. Significant antibacterial activity, especially for ethyl acetate extract, was observed. The best activity was showed against G+ bacteria, Staphylococcus aureus ATCC 25923 and Bacillus subtilis, while Escherichia coli was one of the least sensitive bacteria. Antiproliferative activity of the methanolic extract on HCT- 116 cell line was determined by MTT assay. Results showed that A. flavum has good antiproliferative activity with IC50 values of 28.29 for 24 h and 35.09 for 72 h. Based on these results, A. flavum is a potential source of phenols as natural antioxidant, antibacterial and anticancer substance of high value. Phenolic content of extracts depend on the solvents used for extraction.
authors.affiliation: Department for Biology and Ecology, Faculty of Science, University of Kragujevac, Kragujevac, Serbia. milenagen@gmail.com
authors.forename: Milena G
authors.initials: MG
authors.lastname: Curcic
authors.forename: Milan S
authors.initials: MS
authors.lastname: Stankovic
authors.forename: Ivana D
authors.initials: ID
authors.lastname: Radojevic
authors.forename: Olgica D
authors.initials: OD
authors.lastname: Stefanovic
authors.forename: Ljiljana R
authors.initials: LR
authors.lastname: Comic
authors.forename: Marina D
authors.initials: MD
authors.lastname: Topuzovic
authors.forename: Dragana S
authors.initials: DS
authors.lastname: Djacic
authors.forename: Snezana D
authors.initials: SD
authors.lastname: Markovic
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000032:analysis / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D004926:Escherichia coli / Q000187:drug effects; D005419:Flavonoids / Q000032:analysis* / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D019697:Onions / Q000737:chemistry*; D010636:Phenols / Q000032:analysis / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects; D014407:Tumor Cells, Cultured
chemical_list: D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts