title: Sensitive colorimetric visualization of dihydronicotinamide adenine dinucleotide based on anti-aggregation of gold nanoparticles via boronic acid-diol binding.
abstract: A facile, highly sensitive colorimetric strategy for dihydronicotinamide adenine dinucleotide (NADH) detection is proposed based on anti-aggregation of gold nanoparticles (AuNPs) via boronic acid-diol binding chemistry. The aggregation agent, 4-mercaptophenylboronic acid (MPBA), has specific affinity for AuNPs through Au-S interaction, leading to the aggregation of AuNPs by self-dehydration condensation at a certain concentration, which is responsible for a visible color change of AuNPs from wine red to blue. With the addition of NADH, MPBA would prefer reacting with NADH to form stable borate ester via boronic acid-diol binding dependent on the pH and solvent, revealing an obvious color change from blue to red with increasing the concentration of NADH. The anti-aggregation effect of NADH on AuNPs was seen by the naked eye and monitored by UV-vis extinction spectra. The linear range of the colorimetric sensor for NADH is from 8.0 × 10(-9)M to 8.0 × 10(-6)M, with a low detection limit of 2.0 nM. The as-established colorimetric strategy opened a new avenue for NADH determination.
authors.affiliation: State Key Laboratory Base of Eco-Chemical Engineering, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, People's Republic of China.
authors.forename: Shufeng
authors.initials: S
authors.lastname: Liu
authors.affiliation: State Key Laboratory Base of Eco-Chemical Engineering, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, People's Republic of China.
authors.forename: Zongfeng
authors.initials: Z
authors.lastname: Du
authors.affiliation: State Key Laboratory Base of Eco-Chemical Engineering, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, People's Republic of China.
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.affiliation: State Key Laboratory Base of Eco-Chemical Engineering, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, People's Republic of China; College of Environment and Safety Engineering, Qingdao University of Science and Technology, Qingdao 266042, People's Republic of China. Electronic address: lifeng@qust.edu.cn.
authors.forename: Feng
authors.initials: F
authors.lastname: Li
mesh_terms: D015374:Biosensing Techniques / Q000379:methods* / Q000706:statistics & numerical data; D001897:Boronic Acids; D003124:Colorimetry / Q000379:methods* / Q000706:statistics & numerical data; D006046:Gold; D057230:Limit of Detection; D053768:Metal Nanoparticles; D009243:NAD / Q000032:analysis*; D012680:Sensitivity and Specificity; D013438:Sulfhydryl Compounds
chemical_list: C510906:4-mercaptophenylboronic acid; D001897:Boronic Acids; D013438:Sulfhydryl Compounds; D009243:NAD; D006046:Gold