title: Poly(2-dimethylamino ethylmethacrylate)-based polymers to camouflage red blood cell antigens.
abstract: Poly(2-dimethylamino-ethylmethacrylate) (PDMAEMA) is a cationic polymer when dissolved in a 7.4 pH fluid. Owing to its ionic nature, this polycation interacts with the negatively charged cell membrane surface of red blood cells (RBCs). The electrostatic self-assembly of PDMAEMA on RBCs membrane can be employed for inducing the formation of a polymeric shield camouflaging blood group antigens on RBCs as a valuable strategy for developing "universal RBCs" for blood transfusion. The purpose of this research was to evaluate the camouflaging ability of PDMAEMA homopolymers and PDMAEMA-co-poly(ethylene glycol) copolymers differing in molecular weight and architecture. Surprisingly, the PDMAEMAs caused a partially masking, no masking, and sensitization of the same RBCs population. The MW and architecture of the polymers as well as temperature of PDMAEMA-RBCs treatment influenced the results observed. Herein, the very particular reactivity of PDMAEMAs and RBCs is analyzed and discussed.
authors.affiliation: Interfacultary Research Center of Biomaterials, University of Liège, Institute of Chemistry, Sart-Tillman (Liège), Liège, Belgium.
authors.forename: Bernardino Isaac
authors.initials: BI
authors.lastname: Cerda-Cristerna
authors.forename: Sophie
authors.initials: S
authors.lastname: Cottin
authors.forename: Luca
authors.initials: L
authors.lastname: Flebus
authors.forename: Amaury
authors.initials: A
authors.lastname: Pozos-Guillén
authors.forename: Héctor
authors.initials: H
authors.lastname: Flores
authors.forename: Ernst
authors.initials: E
authors.lastname: Heinen
authors.forename: Olivier
authors.initials: O
authors.lastname: Jolois
authors.forename: Christiane
authors.initials: C
authors.lastname: Gérard
authors.forename: Gianni
authors.initials: G
authors.lastname: Maggipinto
authors.forename: Chantal
authors.initials: C
authors.lastname: Sevrin
authors.forename: Christian
authors.initials: C
authors.lastname: Grandfils
mesh_terms: D001789:Blood Group Antigens / Q000737:chemistry* / Q000276:immunology; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry*; D004912:Erythrocytes / Q000737:chemistry* / Q000276:immunology; D005453:Fluorescence; D006801:Humans; D008689:Methacrylates / Q000138:chemical synthesis / Q000737:chemistry*; D009757:Nylons / Q000138:chemical synthesis / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D001789:Blood Group Antigens; D020099:Coated Materials, Biocompatible; D008689:Methacrylates; D009757:Nylons; C407037:poly(2-(dimethylamino)ethyl methacrylate)