title: Tupisteroide A-C, three new polyhydroxylated steroidal constituents from the roots of Tupistra chinensis.
abstract: Three new steroidal compounds with polyhydroxy groups, tupisteroide A-C (1-3), were obtained from the roots of Tupistra chinensis, together with one known compound (4) that was isolated from this plant for the first time. The structures of tupisteroide A-C were determined on the basis of one- and two-dimensional NMR spectroscopy, including (1) H-(1) H Correlation Spectroscopy, Heteronuclear Multiple Bond Correlation, and Heteronuclear Single Quantum Coherence experiments. The isolated compounds were evaluated for their cytotoxic activities against A549, HepG2, and CaSki cancer cell lines in vitro. Among them, compounds 1, 2, and 4 did not show significant inhibitory activity, but compound 3 showed cytotoxicity against A549 cancer cell lines with IC(50) values of 25.0 μM.
authors.affiliation: Hubei Key Laboratory of Natural Products Research and Development, College of Chemistry and Life Science, China Three Gorges University, Yichang, 443002, China.
authors.forename: Cheng-Xiong
authors.initials: CX
authors.lastname: Liu
authors.forename: Zhi-yong
authors.initials: ZY
authors.lastname: Guo
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Xue
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Zhang
authors.forename: Hong-Qi
authors.initials: HQ
authors.lastname: Zhang
authors.forename: Kun
authors.initials: K
authors.lastname: Zou
authors.forename: Nian-Yu
authors.initials: NY
authors.lastname: Huang
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D006801:Humans; D006914:Hydroxysteroids / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D019668:Liliaceae / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry*; D012503:Saponins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D006914:Hydroxysteroids; D012503:Saponins; C573724:tupisteroide A; C573723:tupisteroide B; C573725:tupisteroide C