title: Qualification and quantification of seventeen natural steroids in plasma by GC-Q-MS and GC-IT-MS/MS.
abstract: Studying the plasma steroid profile offers information about the possible existence of endocrinological alterations. This study describes the development and validation of gas chromatographic-mass spectrometric and gas tandem mass spectrometric methods for the simultaneous identification of 17 steroid hormones in human plasma using five different solvents. The n-hexane/ethyl acetate solvent mixture, in a proportion of 70/30 (v/v) provided the best results. The extracts were derivatized with N-methyl-N-trimethylsilyl-trifluoroacetamide. The obtained limits of detection were below 1 ng/mL in the majority of the studied steroids and the limits of quantification were below 5 ng/mL; the method obtained good linearity, reproducibility, repeatability, accuracy and recoveries above 95% in most cases.
authors.affiliation: Department of Analytical Chemistry, University of Extremadura, Badajoz, Spain. afertd@unex.es
authors.forename: A F
authors.initials: AF
authors.lastname: Toribio-Delgado
authors.forename: M
authors.initials: M
authors.lastname: Maynar-Mariño
authors.forename: M J
authors.initials: MJ
authors.lastname: Caballero-Loscos
authors.forename: M C
authors.initials: MC
authors.lastname: Robles-Gil
authors.forename: G J
authors.initials: GJ
authors.lastname: Olcina-Camacho
authors.forename: J I
authors.initials: JI
authors.lastname: Maynar-Mariño
mesh_terms: D000085:Acetates; D000418:Albumins; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D012739:Gonadal Steroid Hormones / Q000097:blood* / Q000302:isolation & purification; D006586:Hexanes; D006801:Humans; D016014:Linear Models; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000085:Acetates; D000418:Albumins; D012739:Gonadal Steroid Hormones; D006586:Hexanes; C026385:n-hexane; C007650:ethyl acetate