title: Interaction of salivary and midgut proteins of Helicoverpa armigera with soybean trypsin inhibitor.
abstract: Feeding of Helicoverpa armigera larvae on semi-synthetic diet containing Soybean trypsin inhibitor (STI) resulted in disappearance of STI sensitive protease in salivary and midgut protease extract. This might be due to in situ inhibition by dietary STI. STI was largely degraded within 1 h of incubation with total salivary protease (1:1). Degradation was relatively low in midgut proteases. STI interacting proteins were isolated from saliva and midgut extracts of larvae fed on STI supplemented diet using affinity column. Most of the isolated proteins showed caseinolytic activity in zymogram. Denovo sequencing data of seven different peptides selected from trypsin digested total protein showed similarity to chymotrypsinogen, serine protease, aminopeptidase N, peroxidase, hypothetical protein and muscle specific protein.
authors.affiliation: National Botanical Research Institute, Council of Scientific and Industrial Research, Rana Pratap Marg, Lucknow, 226001 UP, India.
authors.forename: Santosh Kumar
authors.initials: SK
authors.lastname: Upadhyay
authors.forename: Krishnappa
authors.initials: K
authors.lastname: Chandrashekar
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D041981:Gastrointestinal Tract / Q000201:enzymology; D019476:Insect Proteins / Q000737:chemistry / Q000378:metabolism*; D007814:Larva / Q000201:enzymology; D007915:Lepidoptera / Q000201:enzymology*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism*; D012463:Saliva / Q000201:enzymology; D030262:Soybean Proteins / Q000008:administration & dosage / Q000378:metabolism*; D014361:Trypsin Inhibitors / Q000008:administration & dosage / Q000378:metabolism*
chemical_list: D019476:Insect Proteins; D010446:Peptide Fragments; D030262:Soybean Proteins; D014361:Trypsin Inhibitors; D010447:Peptide Hydrolases