title: Reverse breeding in Arabidopsis thaliana generates homozygous parental lines from a heterozygous plant.
abstract: Traditionally, hybrid seeds are produced by crossing selected inbred lines. Here we provide a proof of concept for reverse breeding, a new approach that simplifies meiosis such that homozygous parental lines can be generated from a vigorous hybrid individual. We silenced DMC1, which encodes the meiotic recombination protein DISRUPTED MEIOTIC cDNA1, in hybrids of A. thaliana, so that non-recombined parental chromosomes segregate during meiosis. We then converted the resulting gametes into adult haploid plants, and subsequently into homozygous diploids, so that each contained half the genome of the original hybrid. From 36 homozygous lines, we selected 3 (out of 6) complementing parental pairs that allowed us to recreate the original hybrid by intercrossing. In addition, this approach resulted in a complete set of chromosome-substitution lines. Our method allows the selection of a single choice offspring from a segregating population and preservation of its heterozygous genotype by generating homozygous founder lines.
authors.affiliation: Laboratory of Genetics, Wageningen University, Wageningen, The Netherlands. erik.wijnker@wur.nl
authors.forename: Erik
authors.initials: E
authors.lastname: Wijnker
authors.forename: Kees
authors.initials: K
authors.lastname: van Dun
authors.forename: C Bastiaan
authors.initials: CB
authors.lastname: de Snoo
authors.forename: Cilia L C
authors.initials: CL
authors.lastname: Lelivelt
authors.forename: Joost J B
authors.initials: JJ
authors.lastname: Keurentjes
authors.forename: Nazatul Shima
authors.initials: NS
authors.lastname: Naharudin
authors.forename: Maruthachalam
authors.initials: M
authors.lastname: Ravi
authors.forename: Simon W L
authors.initials: SW
authors.lastname: Chan
authors.forename: Hans
authors.initials: H
authors.lastname: de Jong
authors.forename: Rob
authors.initials: R
authors.lastname: Dirks
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics*; D001483:Base Sequence; D001947:Breeding / Q000379:methods*; D018797:Cell Cycle Proteins / Q000235:genetics*; D002678:Chimera / Q000235:genetics; D003433:Crosses, Genetic; D006579:Heterozygote; D006720:Homozygote; D008540:Meiosis / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D034622:RNA Interference; D034741:RNA, Small Interfering; D011938:Rec A Recombinases / Q000235:genetics*; D012639:Seeds / Q000235:genetics; D016415:Sequence Alignment
chemical_list: D029681:Arabidopsis Proteins; D018797:Cell Cycle Proteins; D034741:RNA, Small Interfering; C513034:ATDMC1 protein, Arabidopsis; D011938:Rec A Recombinases