title: Inhibiting CD164 expression in colon cancer cell line HCT116 leads to reduced cancer cell proliferation, mobility, and metastasis in vitro and in vivo.
abstract: BACKGROUND
  CD164 (Endolyn) is a sialomucin, which has been found to play roles in regulating proliferation, adhesion, and differentiation of hematopoietic stem cells. Possible association of CD164 with solid cancer development remains unknown.
  
  
  RESULTS
  We first studied CD164 expression in biopsies from colorectal cancer, breast, and ovary cancer patients by semi-quantitative immunohistochemistry, and found that CD164 was strongly expressed in all the colorectal cancer samples compared to the matching normal colon tissues. The possible roles of CD164 in colon cancer development were further investigated using a well-established human colon cancer cell line HCT116. We found that knockdown of CD164 expression in HCT116 cells significantly inhibited cell proliferation, mobility, and metastasis in vitro and in vivo. The knockdown of CD164 expression was associated with decreased chemokine receptor CXCR4 expression HCT116 cell surface and immunoprecipitation studies showed that CD164 formed complexes with CXCR4.
  
  
  CONCLUSIONS
  CD164 is highly expressed in the colon cancer sites, and it promotes HCT116 colon cancer cell proliferation and metastasis both in vitro and in vivo, and the effects may act through regulating CXCR4 signaling pathway. Therefore, CD164 may be a new target for diagnosis and treatment for colon cancer.
authors.affiliation: Department of Cardiothoracic Surgery, Xiangya Second Hospital, Central South University, Changsha, Hunan, P.R. China.
authors.forename: Jingqun
authors.initials: J
authors.lastname: Tang
authors.forename: Liyang
authors.initials: L
authors.lastname: Zhang
authors.forename: Xiaoling
authors.initials: X
authors.lastname: She
authors.forename: Guangqian
authors.initials: G
authors.lastname: Zhou
authors.forename: Fenglei
authors.initials: F
authors.lastname: Yu
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Xiang
authors.forename: Gang
authors.initials: G
authors.lastname: Li
mesh_terms: D000818:Animals; D002465:Cell Movement; D049109:Cell Proliferation; D054377:Chemokine CXCL12 / Q000502:physiology; D003110:Colonic Neoplasms / Q000473:pathology / Q000628:therapy*; D051930:Endolyn / Q000032:analysis / Q000502:physiology; D005260:Female; D045325:HCT116 Cells; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D009362:Neoplasm Metastasis; D019718:Receptors, CXCR4 / Q000502:physiology
chemical_list: C497898:CD164 protein, human; C515255:CXCL12 protein, human; C527276:CXCR4 protein, human; D054377:Chemokine CXCL12; D051930:Endolyn; D019718:Receptors, CXCR4