title: Influence of HPV16 E6/7 on the expression of FGF2 and FGFR type B in cervical carcinogenesis.
abstract: This study investigates the influence of fibroblast growth factor receptor type B (FGFRb) and fibroblast growth factor on cervical carcinogenesis associated with HPV16 E6/7 infection, using primary cancer cells isolated from Taiwanese patients with cervical cancer. Functional interaction between FGFRb in Cx cells and HPV16 E6/7 transfected Cx cells (CxWJ cells) following treatment with FGF-7, according to cell growth, invasive ability, and tumor growth in SCID mice. Our results indicate that the downregulation of FGFRb gene expression in CxWJ cells partially represses proliferation and the invasive ability provided by FGF-7 stimulation. In SCID mice, the FGF2 and FGFR1 gene expression ascend in CxWJ tumor nodule. These data provide evidence of a functional interaction between HPV16 E6/7 in FGFRb and FGF2, suggesting that cooperative stimulation of HPV E6/7 in inactivated FGFRb and the upregulation of FGF2 may be necessary to completely overcome the oncogenic function associated with the progression of cervical carcinogenesis.
authors.affiliation: Department of Obstetrics and Gynecology, College of Medicine, National Cheng Kung University Hospital, Tainan, Taiwan. chengym0687@gmail.com
authors.forename: Ya-Min
authors.initials: YM
authors.lastname: Cheng
authors.forename: Cheng-Yang
authors.initials: CY
authors.lastname: Chou
authors.forename: Yi-Chiang
authors.initials: YC
authors.lastname: Hsu
authors.forename: Ming-Jenn
authors.initials: MJ
authors.lastname: Chen
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000235:genetics*; D052162:Human papillomavirus 16; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000502:physiology; D050725:Papillomavirus E7 Proteins / Q000235:genetics / Q000502:physiology; D030361:Papillomavirus Infections / Q000378:metabolism* / Q000473:pathology; D016133:Polymerase Chain Reaction; D017468:Receptors, Fibroblast Growth Factor / Q000235:genetics*; D012097:Repressor Proteins / Q000235:genetics / Q000502:physiology; D014162:Transfection; D002583:Uterine Cervical Neoplasms / Q000378:metabolism / Q000473:pathology / Q000821:virology*
chemical_list: C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D017468:Receptors, Fibroblast Growth Factor; D012097:Repressor Proteins; C059731:oncogene protein E7, Human papillomavirus type 16; D016222:Fibroblast Growth Factor 2