title: An efficient and simple method for site-selective modification of O6-methyl-2'-deoxyguanosine in DNA.
abstract: O(6)-Methyl-2'-deoxyguanosine (O(6)-Me-dG) is a mutagenic nucleotide in DNA. O(6)-Me-dG in DNA was rapidly and selectively modified by a functionality transfer reaction using the ODN incorporating 6-S-functionalized thioguanosine. Subsequent labelling of O(6)-Me-dG with the fluorescent FAM or biotin group via click chemistry has permitted the sensitive and selective detection of O(6)-Me-dG in DNA.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
authors.forename: Kazumitsu
authors.initials: K
authors.lastname: Onizuka
authors.forename: Takamasa
authors.initials: T
authors.lastname: Nishioka
authors.forename: Zhichun
authors.initials: Z
authors.lastname: Li
authors.forename: Daichi
authors.initials: D
authors.lastname: Jitsuzaki
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Taniguchi
authors.forename: Shigeki
authors.initials: S
authors.lastname: Sasaki
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000737:chemistry; D013379:Substrate Specificity
chemical_list: D004247:DNA; C015558:O(6)-methyl-2'-deoxyguanosine; D003849:Deoxyguanosine