title: Development of fibroblast culture in three-dimensional activated carbon fiber-based scaffold for wound healing.
abstract: This work developed a novel bi-layer wound dressing composed of 3D activated carbon fibers that allows facilitates fibroblast cell growth and migration to a wound site for tissue reconstruction, and the gentamicin is incorporated into a poly(γ-glutamic acid)/gelatin membrane to prevent bacterial infection. In an in vitro, field emission scanning electron microscopy shows that rat skin fibroblasts appeared and spread on the surface of activated carbon fibers, and penetrated the interior and exterior of the 3D activated carbon fiber construct to a depth of roughly 200 μm. An in vivo analysis shows that fibroblast cells containing the proposed 3D scaffold had the potential of a biologically functionalized dressing to accelerate wound closure. Additionally, fibroblasts migrated to the wound site in a bi-layer wound dressing containing fibroblasts, enhancing fibronectin and type I collagen expression, resulting in faster skin regeneration than that achieved with a Tegaderm™ hydrocolloid dressing or gauze.
authors.affiliation: Department of Applied Cosmetology and Graduate Institute of Cosmetic Science, Hungkuang University, Taichung, Taiwan.
authors.forename: Wen-Ying
authors.initials: WY
authors.lastname: Huang
authors.forename: Chia-Lin
authors.initials: CL
authors.lastname: Yeh
authors.forename: Jui-Hsiang
authors.initials: JH
authors.lastname: Lin
authors.forename: Jai-Sing
authors.initials: JS
authors.lastname: Yang
authors.forename: Tse-Hao
authors.initials: TH
authors.lastname: Ko
authors.forename: Yu-Hsin
authors.initials: YH
authors.lastname: Lin
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage; D001672:Biocompatible Materials / Q000737:chemistry; D001684:Biological Dressings; D002244:Carbon / Q000737:chemistry; D000077482:Carbon Fiber; D018929:Cell Culture Techniques; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology* / Q000502:physiology*; D005353:Fibronectins / Q000378:metabolism; D005839:Gentamicins / Q000008:administration & dosage; D008297:Male; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000293:injuries / Q000473:pathology; D012879:Skin Physiological Phenomena; D054457:Tissue Scaffolds / Q000737:chemistry; D014945:Wound Healing / Q000502:physiology*
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D000077482:Carbon Fiber; D024042:Collagen Type I; D005353:Fibronectins; D005839:Gentamicins; D002244:Carbon