title: Cell viability and immunostimulating and protective capacities of Bifidobacterium longum 51A are differentially affected by technological variables in fermented milks.
abstract: OBJECTIVE
  To investigate the cell viability of Bifidobacterium longum 5(1A) in fermented milks and to study its immunostimulating and protective capacity against Salmonella enterica ssp. enterica serovar Typhimurium infection in mice.
  
  
  RESULTS
  Bifidobacterium longum 5(1A) was added to milk fermented with different yoghurt starter cultures, before or after fermentation, and viability was monitored during storage (5°C, 28 days). Resistance to simulated gastric acid digestion was assessed. Fermented milks were orally administered to mice for 10 days followed by oral infection with Salmonella Typhimurium. The number of IgA+ cells in the small and large intestine was determined before infection. Survival to infection was monitored for 20 days. Bifidobacterium longum 5(1A) lost viability during storage, but the product containing it was effective for the induction of IgA+ cells proliferation in the gut and for the protection of mice against Salm. Typhimurium infection.
  
  
  CONCLUSIONS
  Cell viability of Bif. longum 5(1A) in fermented milks along storage did not condition the capacity of the strain to enhance the number of IgA+ cells in the gut and to protect mice against Salmonella infection.
  
  
  CONCLUSIONS
  The uncoupling of cell viability and functionality demonstrated that, in certain cases, nonviable cells can also exert positive effects.
authors.affiliation: Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: T C
authors.initials: TC
authors.lastname: Souza
authors.forename: M F
authors.initials: MF
authors.lastname: Zacarías
authors.forename: A M
authors.initials: AM
authors.lastname: Silva
authors.forename: A
authors.initials: A
authors.lastname: Binetti
authors.forename: J
authors.initials: J
authors.lastname: Reinheimer
authors.forename: J R
authors.initials: JR
authors.lastname: Nicoli
authors.forename: G
authors.initials: G
authors.lastname: Vinderola
mesh_terms: D000818:Animals; D001644:Bifidobacterium / Q000502:physiology*; D005285:Fermentation; D007114:Immunization; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D050296:Microbial Viability; D008892:Milk / Q000737:chemistry / Q000382:microbiology*; D019936:Probiotics / Q000008:administration & dosage*; D012480:Salmonella Infections / Q000276:immunology / Q000517:prevention & control; D012486:Salmonella typhimurium / Q000276:immunology