title: Recombinant influenza A viruses with enhanced levels of PB1 and PA viral protein expression.
abstract: Influenza A viruses containing the promoter mutations G3A/C8U in a given segment express increased levels of the corresponding viral protein during infection due to increased levels of mRNA or cRNA species. The replication of these recombinant viruses is attenuated, and they have an enhanced shedding of noninfectious particles and are incapable of antagonizing interferon (IFN) effectively. Our findings highlight the possibility of increasing influenza virus protein expression and the need for a delicate balance between influenza viral replication, protein expression, and assembly.
authors.affiliation: Department of Microbiology, Mount Sinai School of Medicine, New York, New York, USA.
authors.forename: Alan
authors.initials: A
authors.lastname: Belicha-Villanueva
authors.forename: Juan R
authors.initials: JR
authors.lastname: Rodriguez-Madoz
authors.forename: Jad
authors.initials: J
authors.lastname: Maamary
authors.forename: Alina
authors.initials: A
authors.lastname: Baum
authors.forename: Dabeiba
authors.initials: D
authors.lastname: Bernal-Rubio
authors.forename: Maria
authors.initials: M
authors.lastname: Minguito de la Escalera
authors.forename: Ana
authors.initials: A
authors.lastname: Fernandez-Sesma
authors.forename: Adolfo
authors.initials: A
authors.lastname: García-Sastre
mesh_terms: D015967:Gene Expression Regulation, Viral; D006801:Humans; D009980:Influenza A virus / Q000201:enzymology* / Q000235:genetics* / Q000502:physiology; D007251:Influenza, Human / Q000821:virology*; D009154:Mutation; D011401:Promoter Regions, Genetic; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication
chemical_list: C061290:PA protein, influenza viruses; D014764:Viral Proteins; C077346:influenza virus polymerase basic protein 1; D012324:RNA-Dependent RNA Polymerase