title: Association between gout and polymorphisms in GCKR in male Han Chinese.
abstract: Several genome-wide association studies (GWASs) have reported associations between single nucleotide polymorphisms (SNPs) and uric acid concentrations or gout in a number of different ethnic populations. To clarify the global relevance of the previously identified SNPs in the development of the qualitative trait gout, in the present study, the associations between two SNPs in the glucokinase (hexokinase 4) regulator (GCKR) gene and gout were assessed in a male Chinese Han population. The study population comprised 476 male gout patients and 465 male controls. Multiple PCR was performed using time-of-flight mass spectrometry (MALDI-TOF MS) to identify genotypes. Two SNPs, rs780093 and rs780094, located in intronic regions of the GCKR gene were found to be significantly associated with the development of gout. Thus, the association between the two GCKR SNPs and gout was replicated in the male Han Chinese population investigated in the present study. Furthermore, GCKR was identified as a novel candidate gene associated with gout.
authors.affiliation: Graduate School, Peking Union Medical College, Beijing, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Shiguo
authors.initials: S
authors.lastname: Liu
authors.forename: Binbin
authors.initials: B
authors.lastname: Wang
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Miao
authors.forename: Lin
authors.initials: L
authors.lastname: Han
authors.forename: Nan
authors.initials: N
authors.lastname: Chu
authors.forename: Kun
authors.initials: K
authors.lastname: Zhang
authors.forename: Dongmei
authors.initials: D
authors.lastname: Meng
authors.forename: Changgui
authors.initials: C
authors.lastname: Li
authors.forename: Xu
authors.initials: X
authors.lastname: Ma
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics*; D044466:Asian People / Q000235:genetics*; D001786:Blood Glucose / Q000032:analysis; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D002784:Cholesterol / Q000097:blood; D003404:Creatinine / Q000097:blood; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006073:Gout / Q000150:complications / Q000208:ethnology* / Q000235:genetics*; D006801:Humans; D006973:Hypertension / Q000150:complications; D033461:Hyperuricemia / Q000150:complications; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000150:complications; D020641:Polymorphism, Single Nucleotide; D014280:Triglycerides / Q000097:blood; D014527:Uric Acid / Q000097:blood
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D001786:Blood Glucose; C485100:GCKR protein, human; D014280:Triglycerides; D014527:Uric Acid; D002784:Cholesterol; D003404:Creatinine