title: Aberrant expression of miR-196a in gastric cancers and correlation  with recurrence.
abstract: MicroRNAs (miRNAs) are short noncoding RNAs (~22 nt) that play important roles in the pathogenesis of human diseases by negatively regulating gene expression. Here, we examined the relationship between miR-196a and gastric cancer.By the analysis of 72 gastric cancer samples, we found that the expression level of miR-196a microRNA significantly increased in primary gastric cancer tissues versus adjacent normal tissues. In addition, extracellular miR-196a detected in conditioned medium was strongly correlated with its cellular expression status and increased circulating miR-196a in patient serum was associated with gastric cancer disease status and relapse. Furthermore, ectopic expression of miR-196a microRNA promoted the epithelial-mesenchymal transition and migration/invasion capabilities of transfected cells, suggesting its oncogenic potential in gastric cancer progression. Altogether, our data demonstrate that miR-196a exerts an oncogenic role in gastric cancer and miR-196a may be a novel biomarker for detecting gastric cancer and for monitoring disease recurrence.
authors.affiliation: Department of Medical Education and Research, KaohsiungVeterans General Hospital, Kaohsiung, Taiwan, Republic of China.
authors.forename: Kuo-Wang
authors.initials: KW
authors.lastname: Tsai
authors.forename: Yu-Lun
authors.initials: YL
authors.lastname: Liao
authors.forename: Chew-Wun
authors.initials: CW
authors.lastname: Wu
authors.forename: Ling-Yueh
authors.initials: LY
authors.lastname: Hu
authors.forename: Sung-Chou
authors.initials: SC
authors.lastname: Li
authors.forename: Wen-Ching
authors.initials: WC
authors.lastname: Chan
authors.forename: Meng-Ru
authors.initials: MR
authors.lastname: Ho
authors.forename: Chun-Hung
authors.initials: CH
authors.lastname: Lai
authors.forename: Hsiao-Wei
authors.initials: HW
authors.lastname: Kao
authors.forename: Wen-Liang
authors.initials: WL
authors.lastname: Fang
authors.forename: Kuo-Hung
authors.initials: KH
authors.lastname: Huang
authors.forename: Wen-Chang
authors.initials: WC
authors.lastname: Lin
mesh_terms: D014408:Biomarkers, Tumor; D002465:Cell Movement; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis / Q000097:blood / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics; D009364:Neoplasm Recurrence, Local / Q000235:genetics*; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D014407:Tumor Cells, Cultured
chemical_list: D014408:Biomarkers, Tumor; C542543:MIRN196 microRNA, human; D035683:MicroRNAs