title: Combining platelet-rich plasma and tissue-engineered skin in the treatment of large skin wound.
abstract: The objective of the study was to observe the effects of tissue-engineered skin in combination with platelet-rich plasma (PRP) and other preparations on the repair of large skin wound on nude mice.We first prepared PRP from venous blood by density-gradient centrifugation. Large skin wounds were created surgically on the dorsal part of nude mice. The wounds were then treated with either artificial skin, tissue-engineered skin, tissue-engineered skin combined with basic fibroblast growth factor, tissue-engineered skin combined with epidermal growth factor, or tissue-engineered skin combined with PRP. Tissue specimens were collected at different time intervals after surgery. Hematoxylin-eosin and periodic acid-Schiff staining and immunohistochemistry were performed to assess the rate of wound healing.Macroscopic observations, hematoxylin-eosin/periodic acid-Schiff staining, and immunohistochemistry revealed that the wounds treated with tissue-engineered skin in combination with PRP showed the most satisfactory wound recovery, among the 5 groups.
authors.affiliation: Division of Plastic Surgery, Ji-nan Military Hospital, Ji-nan, China. hantong8410@sina.com.cn
authors.forename: Tong
authors.initials: T
authors.lastname: Han
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.forename: Ya Qin
authors.initials: YQ
authors.lastname: Zhang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D004815:Epidermal Growth Factor / Q000097:blood; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D010976:Platelet Count; D010982:Platelet-Derived Growth Factor / Q000378:metabolism; D053657:Platelet-Rich Plasma; D012867:Skin / Q000293:injuries*; D017768:Skin, Artificial; D013997:Time Factors; D023822:Tissue Engineering; D053773:Transforming Growth Factor beta1 / Q000097:blood; D014945:Wound Healing / Q000187:drug effects / Q000502:physiology
chemical_list: D010982:Platelet-Derived Growth Factor; D053773:Transforming Growth Factor beta1; D004815:Epidermal Growth Factor