title: Associations between polymorphisms in genes involved in fatty acid metabolism and dietary fat intakes.
abstract: BACKGROUND
  Obesity prevalence is growing in our population. Twin studies have estimated the heritability of dietary intakes to about 30%. The objective of this study was to verify whether polymorphisms in genes involved in fatty acid metabolism are associated with dietary fat intakes.
  
  
  METHODS
  Seven hundred participants were recruited. A validated food frequency questionnaire was used to assess dietary intakes. PCR-RFLP and TAQMAN methodology were used to genotype PPARα Leu162Val, PPARγ Pro12Ala, PPARδ -87T>C, PPARGC1α Gly482Ser, FASN Val1483Ile and SREBF1 c.*619C>G. Statistical analyses were executed with SAS statistical package.
  
  
  RESULTS
  Carriers of the Ala12 allele of PPARγ Pro12Ala polymorphism had higher intakes of total fat (p = 0.04). For FASN Val1483Ile polymorphism, significant gene-sex interaction effects were found for total fat and saturated fat intakes (p = 0.02 and p = 0.002, respectively). No significant difference in fat intakes was observed for PPARα Leu162Val, PPARδ -87T>C, PPARGC1α Gly482Ser and SREBF1 c.*619C>G polymorphisms.
  
  
  CONCLUSIONS
  Polymorphisms in PPARγ and FASN seem to be associated with dietary fat intakes. Genetic variants are important to take into account when studying dietary intakes.
authors.affiliation: Institute of Nutraceuticals and Functional Foods-INAF, Quebec, Que., Canada.
authors.forename: Annie
authors.initials: A
authors.lastname: Bouchard-Mercier
authors.forename: Ann-Marie
authors.initials: AM
authors.lastname: Paradis
authors.forename: Louis
authors.initials: L
authors.lastname: Pérusse
authors.forename: Marie-Claude
authors.initials: MC
authors.lastname: Vohl
mesh_terms: D000328:Adult; D004041:Dietary Fats / Q000008:administration & dosage*; D005227:Fatty Acids / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D011792:Quebec; D020133:Reverse Transcriptase Polymerase Chain Reaction; D011795:Surveys and Questionnaires
chemical_list: D004041:Dietary Fats; D005227:Fatty Acids