title: Preservation of (-)-epigallocatechin-3-gallate antioxidant properties loaded in heat treated β-lactoglobulin nanoparticles.
abstract: (-)-Epigallocatechin-3-gallate (EGCG) was loaded in heat treated β-lactoglobulin (β-Lg) for the preservation of antioxidant activity. The effects of pH (2.5-7.0), the heating temperature of β-Lg (30-85 °C), the molar ratio of β-Lg to EGCG (1:2-1:32), and the β-Lg concentration (1-10 mg/mL) on the properties of β-Lg-EGCG complexes were studied. All four factors significantly influenced the particle size, the ζ-potential, and the entrapment efficiency of EGCG and EGCG loading in β-Lg particles. A stable and clear solution system could be obtained at pH 6.4-7.0. The highest protection of EGCG antioxidant activity was obtained with β-Lg heated at 85 °C and the molar ratio of 1:2 (β-Lg: EGCG). β-Lg-EGCG complexes were found to have the same secondary structure as native β-Lg.
authors.affiliation: Institute of Food and Biological Engineering, Zhejiang Gongshang University, Hangzhou, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.forename: Wenkai
authors.initials: W
authors.lastname: Du
authors.forename: Jianchang
authors.initials: J
authors.lastname: Jin
authors.forename: Qizhen
authors.initials: Q
authors.lastname: Du
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D002392:Catechin / Q000031:analogs & derivatives* / Q000737:chemistry; D004355:Drug Stability; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007782:Lactoglobulins / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D017433:Protein Structure, Secondary
chemical_list: D000975:Antioxidants; D007782:Lactoglobulins; D002392:Catechin; C045651:epigallocatechin gallate