title: Proteomic analysis of soybean defense response induced by cotton worm (prodenia litura, fabricius) feeding.
abstract: BACKGROUND
  Cotton worm is one of the main insects of soybean in southern China. Plants may acquire defense mechanisms that confer protection from predation by herbivores. Induced responses can lead to increased resistance against herbivores in many species. This study focuses on searching changed proteins in soybean defense response induced by cotton worm feeding.
  
  
  RESULTS
  Ten protein spots that are changed in abundance in response to cotton worm feeding were identified by Two-dimensional gel electrophoresis (2-DE). A total of 11 unique proteins from these spots were identified by MALDI-TOF MS. The mRNA and protein relative expression levels of most changed proteins were up-regulated. These proteins were mainly involved in physiological processes, including active oxygen removal, defense signal transduction, and metabolism regulation.
  
  
  CONCLUSIONS
  This is the first proteomic analysis of the soybean defense response induced by cotton worm. The differentially expressed proteins could work together to play a major role in the induced defense response. PAL and SAMS were up-regulated at both the protein and mRNA levels. These genes can be strongest candidates for further functional research.
authors.affiliation: National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China. dyyu@njau.edu.cn.
authors.forename: Rui
authors.initials: R
authors.lastname: Fan
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Yongli
authors.initials: Y
authors.lastname: Wang
authors.forename: Deyue
authors.initials: D
authors.lastname: Yu