title: Petalonia binghamiae extract and its constituent fucoxanthin ameliorate high-fat diet-induced obesity by activating AMP-activated protein kinase.
abstract: In this study, we investigated the antiobesity properties of  Petalonia binghamiae  extract (PBE) in mice in which obesity was induced with a high-fat diet (HFD). PBE administration (150 mg/kg/day) for 70 days decreased body weight gain, adipose tissue weight, and the serum triglyceride level in mice fed a HFD. PBE reduced serum levels of glutamic pyruvic transaminase and glutamic oxaloacetic transaminase as well as the accumulation of lipid droplets in the liver. PBE restored the HFD-induced decrease in phosphorylation of AMP-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC) in epididymal adipose tissue. PBE increased the phosphorylation of AMPK and ACC and decreased the expression of SREBP1c in mature 3T3-L1 adipocytes. In addition, we further explored the active compound responsible for AMPK activation by PBE in 3T3-L1 adipocytes. Fucoxanthin isolated from PBE increased the phosphorylation of AMPK and ACC with increasing LKB1 phosphorylation in mature 3T3-L1 adipocytes. Taken together, these data suggest that PBE (or fucoxanthin) exert improving effects on HFD-induced obesity by promoting β-oxidation and reducing lipogenesis.
authors.affiliation: Department of Biology, Jeju National University, Jejusi, Jeju Special Self-Governing province, Republic of Korea.
authors.forename: Seong-Il
authors.initials: SI
authors.lastname: Kang
authors.forename: Hye-Sun
authors.initials: HS
authors.lastname: Shin
authors.forename: Hyo-Min
authors.initials: HM
authors.lastname: Kim
authors.forename: Seon-A
authors.initials: SA
authors.lastname: Yoon
authors.forename: Seung-Woo
authors.initials: SW
authors.lastname: Kang
authors.forename: Jeong-Hwan
authors.initials: JH
authors.lastname: Kim
authors.forename: Hee-Chul
authors.initials: HC
authors.lastname: Ko
authors.forename: Se-Jae
authors.initials: SJ
authors.lastname: Kim
mesh_terms: D016475:3T3 Cells; D055372:AMP-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism*; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D019440:Anti-Obesity Agents / Q000008:administration & dosage*; D001685:Biological Factors / Q000008:administration & dosage*; D001835:Body Weight / Q000187:drug effects; D059305:Diet, High-Fat / Q000009:adverse effects; D004435:Eating / Q000187:drug effects; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000188:drug therapy* / Q000201:enzymology / Q000378:metabolism / Q000503:physiopathology; D000459:Phaeophyceae / Q000737:chemistry*; D014280:Triglycerides / Q000378:metabolism; D024341:Xanthophylls / Q000008:administration & dosage*
chemical_list: D019440:Anti-Obesity Agents; D001685:Biological Factors; D014280:Triglycerides; D024341:Xanthophylls; C025164:fucoxanthin; D055372:AMP-Activated Protein Kinases