title: Evaluation of the GenBank, EzTaxon, and BIBI services for molecular identification of clinical blood culture isolates that were unidentifiable or misidentified by conventional methods.
abstract: We compared the 16S rRNA gene sequencing results analyzed with the GenBank, EzTaxon, and BIBI databases for blood culture specimens for which identifications were incomplete, conflicting, or unidentifiable using conventional methods. Analyses performed using GenBank combined with EzTaxon (kappa = 0.79) were more discriminative than those using other databases alone or in combination with a second database.
authors.affiliation: Department of Laboratory Medicine & Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea.
authors.forename: Kyung Sun
authors.initials: KS
authors.lastname: Park
authors.forename: Chang-Seok
authors.initials: CS
authors.lastname: Ki
authors.forename: Cheol-In
authors.initials: CI
authors.lastname: Kang
authors.forename: Yae-Jean
authors.initials: YJ
authors.lastname: Kim
authors.forename: Doo Ryeon
authors.initials: DR
authors.lastname: Chung
authors.forename: Kyong Ran
authors.initials: KR
authors.lastname: Peck
authors.forename: Jae-Hoon
authors.initials: JH
authors.lastname: Song
authors.forename: Nam Yong
authors.initials: NY
authors.lastname: Lee
mesh_terms: D016470:Bacteremia / Q000175:diagnosis* / Q000382:microbiology*; D001419:Bacteria / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D001769:Blood / Q000382:microbiology*; D019295:Computational Biology / Q000379:methods*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D030561:Databases, Nucleic Acid; D006801:Humans; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S