title: Current challenges in detecting food allergens by shotgun and targeted proteomic approaches: a case study on traces of peanut allergens in baked cookies.
abstract: There is a need for selective and sensitive methods to detect the presence of food allergens at trace levels in highly processed food products. In this work, a combination of non-targeted and targeted proteomics approaches are used to illustrate the difficulties encountered in the detection of the major peanut allergens Ara h 1, Ara h 2 and Ara h 3 from a representative processed food matrix. Shotgun proteomics was employed for selection of the proteotypic peptides for targeted approaches via selective reaction monitoring. Peanut presence through detection of the proteotypic Ara h 3/4 peptides AHVQVVDSNGNR (m/z 432.5, 3+) and SPDIYNPQAGSLK (m/z 695.4, 2+) was confirmed and the developed method was able to detect peanut presence at trace levels (≥10 μg peanut g(-1) matrix) in baked cookies.
authors.affiliation: European Commission (EC), DG Joint Research Centre (JRC), Institute for Reference Materials and Measurements (IRMM), Geel 2440, Belgium. romina.pedreschi@ec.europa.eu
authors.forename: Romina
authors.initials: R
authors.lastname: Pedreschi
authors.forename: Jørgen
authors.initials: J
authors.lastname: Nørgaard
authors.forename: Alain
authors.initials: A
authors.lastname: Maquet
mesh_terms: D055516:2S Albumins, Plant / Q000032:analysis; D000485:Allergens / Q000032:analysis*; D052179:Antigens, Plant / Q000032:analysis; D010367:Arachis / Q000235:genetics*; D005504:Food Analysis / Q000379:methods*; D005511:Food Handling; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D008565:Membrane Proteins; D010940:Plant Proteins / Q000032:analysis; D040901:Proteomics / Q000379:methods*
chemical_list: D055516:2S Albumins, Plant; D000485:Allergens; D052179:Antigens, Plant; C500889:Ara h 1 protein, Arachis hypogaea; C500890:Ara h 2 allergen, Arachis hypogaea; C549941:Ara h 3 allergen, Arachis hypogea; D006023:Glycoproteins; D008565:Membrane Proteins; D010940:Plant Proteins