title: Comment on "Widespread RNA and DNA sequence differences in the human transcriptome".
abstract: Li et al. (Research Articles, 1 July 2011, p. 53; published online 19 May 2011) reported widespread differences between the RNA and DNA sequences of the same human cells, including all 12 possible mismatch types. Before accepting such a fundamental claim, a deeper analysis of the sequencing data is required to discern true differences between RNA and DNA from potential artifacts.
authors.affiliation: Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Lin
authors.forename: Robert
authors.initials: R
authors.lastname: Piskol
authors.forename: Meng How
authors.initials: MH
authors.lastname: Tan
authors.forename: Jin Billy
authors.initials: JB
authors.lastname: Li
mesh_terms: D004247:DNA / Q000235:genetics*; D014644:Genetic Variation; D015894:Genome, Human; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D004247:DNA