title: DNA extraction from crayfish exoskeleton.
abstract: Crayfish exoskeleton (CE) samples are generally less invasive and easy to be collected. However, it is difficult to extract DNA from them. This study was intended to investigate CE as a DNA source and design an easy and efficient DNA extraction protocol for polymerase chain reactions. Specific primer pair (PPO-F, PPO-R) was used to amplify extracted DNA from CE, and compared to crayfish tail muscle DNA sample. Moreover, seven microsatellites markers were used to amplify the CE DNA samples set. Since the extracted DNA from CE is suitable for gene amplification, the results present usefulness of CE as an easy and convenient DNA source for PCR-based population genetic research.
authors.affiliation: College of Fisheries, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, P.R.China.
authors.forename: Yanhe
authors.initials: Y
authors.lastname: Li
authors.forename: Weimin
authors.initials: W
authors.lastname: Wang
authors.forename: Xiaolian
authors.initials: X
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Luo
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Yasmeen
authors.initials: Y
authors.lastname: Gul
mesh_terms: D060105:Animal Shells / Q000737:chemistry*; D000818:Animals; D003400:Astacoidea / Q000235:genetics*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D005828:Genetics, Population / Q000379:methods*; D006579:Heterozygote; D018895:Microsatellite Repeats / Q000235:genetics; D009132:Muscles / Q000737:chemistry; D011110:Polymorphism, Genetic; D012863:Skeleton; D013053:Spectrophotometry
chemical_list: D004247:DNA