title: Development of sequence characterized amplified region from random amplified polymorphic DNA amplicons.
abstract: Among the PCR-based markers that are most widely used in molecular genetic studies, SCARs (sequence characterized amplified regions) have the advantage of being less sensitive to the conditions of a standard PCR due to its primer size when compared to RAPD (random amplified polymorphic DNA) and hence are more specific and reproducible. Moreover, SCARs require no radioactive isotopes and detect only a single locus. Here, we describe the development of SCAR from RAPD amplicons.
authors.affiliation: Department of Biotechnology, Sinhgad College of Engineering, Pune, India. joshikalpana@gmail.com
authors.forename: Kalpana
authors.initials: K
authors.lastname: Joshi
authors.forename: Preeti
authors.initials: P
authors.lastname: Chavan
mesh_terms: D017931:DNA Primers / Q000737:chemistry*; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers