title: Poly(ethyleneimine)-mediated large-scale transient gene expression: influence of molecular weight, polydispersity and N-propionyl groups.
abstract: Three synthesis lots of linear poly(ethyleneimine) (PEI) are compared to a fully hydrolyzed linear PEI (commercially available as PEI "Max") regarding structure, polyplex formation with plasmid DNA, and transfection of suspension-adapted HEK-293E cells. PEI "Max" binds DNA more efficiently than the other PEIs, but it is the least effective in terms of transient recombinant protein yield. One PEI lot is fractionated by means of SEC. The fractions of high-M(n) PEI are the most efficient for complex formation and transfection. Nevertheless, the highest transient recombinant protein yields are achieved with unfractionated PEI. The results demonstrate that the polydispersity and charge density of linear PEI are important parameters for gene delivery to suspension-adapted HEK-293E cells.
authors.affiliation: Laboratoire des Polymères, Institut des Matériaux et Institut des Sciences et Ingénieries Chimiques, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Kadlecova
authors.forename: Sophie
authors.initials: S
authors.lastname: Nallet
authors.forename: David L
authors.initials: DL
authors.lastname: Hacker
authors.forename: Lucia
authors.initials: L
authors.lastname: Baldi
authors.forename: Harm-Anton
authors.initials: HA
authors.lastname: Klok
authors.forename: Florian M
authors.initials: FM
authors.lastname: Wurm
mesh_terms: D005591:Chemical Fractionation; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D015870:Gene Expression; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D057809:HEK293 Cells; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008856:Microscopy, Fluorescence; D008970:Molecular Weight; D010957:Plasmids / Q000737:chemistry* / Q000235:genetics; D011094:Polyethyleneimine / Q000737:chemistry*; D055672:Static Electricity; D014162:Transfection
chemical_list: C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D011094:Polyethyleneimine; D004247:DNA