title: Nontargeted nucleotide analysis based on benzoylhistamine labeling-MALDI-TOF/TOF-MS: discovery of putative 6-oxo-thymine in DNA.
abstract: A method for nontargeted analysis of modified nucleotides in DNA (and RNA) is reported based on labeling with benzoylhistamine (BH) followed by MALDI-MS. The method provides deoxynucleotide-specific detection, accurate measurement of molecular ions, high sensitivity, semiquantitation, and, to the extent studied to date, normalization of response within a factor of <3.
authors.affiliation: Pharmaceutical Science and Barnett Institute, Northeastern University, Boston, Massachusetts 02115, United States.
authors.forename: Poguang
authors.initials: P
authors.lastname: Wang
authors.forename: David
authors.initials: D
authors.lastname: Fisher
authors.forename: Anjana
authors.initials: A
authors.lastname: Rao
authors.forename: Roger W
authors.initials: RW
authors.lastname: Giese
mesh_terms: D004247:DNA / Q000737:chemistry*; D005260:Female; D006632:Histamine / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D009711:Nucleotides / Q000032:analysis*; D010920:Placenta / Q000737:chemistry; D011247:Pregnancy; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013194:Staining and Labeling; D013941:Thymine / Q000737:chemistry*
chemical_list: D009711:Nucleotides; D006632:Histamine; D004247:DNA; D013941:Thymine