title: Decellularized rabbit cricoarytenoid dorsalis muscle for laryngeal regeneration.
abstract: OBJECTIVE
  Although considerable progress has been made in regenerative medicine, a quantum step would be the replacement and/or regeneration of functional muscle tissue. For example, although patients' airways can now be successfully replaced with stem cell-based techniques, a much greater patient need would be addressed by regeneration of the muscles required for engineering a functional larynx, in which active movement is critical. The rabbit cricoarytenoid dorsalis muscle was chosen for the present study because it is equivalent to the posterior cricoarytenoid muscle, the only significant abductor muscle in human larynges.
  
  
  METHODS
  Rabbit cricoarytenoid dorsalis muscles were harvested, and different decellularization methods were compared by use of a combination of histologic, immunohistochemical, and molecular techniques. Decellularized scaffolds were implanted into Sprague-Dawley rats as part of a 2-week biocompatibility study to assess immunogenicity.
  
  
  RESULTS
  Decellularization with a combination of latrunculin B, potassium iodide, potassium chloride, and deoxyribonuclease resulted in total DNA clearance and reduced levels of major histocompatibility complex class II expression, with relative preservation of the scaffold's structural integrity (collagen, elastin, and glycosaminoglycan content). The scaffolds showed minimal signs of rejection at 2 weeks in a cross-species (xenotransplantation) study.
  
  
  CONCLUSIONS
  Decellularized laryngeal muscles, which are nonimmunogenic, may provide the optimal scaffold source for the generation of a fully functional tissue-engineered larynx.
authors.affiliation: University College London Institute of Child Health, 30 Guilford St, London WC1N 1EH, United Kingdom.
authors.forename: Jonathan M
authors.initials: JM
authors.lastname: Fishman
authors.forename: Tahera
authors.initials: T
authors.lastname: Ansari
authors.forename: Paul
authors.initials: P
authors.lastname: Sibbons
authors.forename: Paolo
authors.initials: P
authors.lastname: De Coppi
authors.forename: Martin A
authors.initials: MA
authors.lastname: Birchall
mesh_terms: D000818:Animals; D019086:Bridged Bicyclo Compounds, Heterocyclic; D004247:DNA / Q000032:analysis; D003851:Deoxyribonucleases; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D007150:Immunohistochemistry; D007821:Laryngeal Muscles / Q000378:metabolism / Q000637:transplantation* / Q000648:ultrastructure; D007830:Larynx / Q000502:physiology*; D008853:Microscopy; D011189:Potassium Chloride; D011193:Potassium Iodide; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration; D012967:Sodium Dodecyl Sulfate; D013501:Surface-Active Agents; D053778:Thiazolidines; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014183:Transplantation, Heterologous
chemical_list: D019086:Bridged Bicyclo Compounds, Heterocyclic; D000949:Histocompatibility Antigens Class II; D013501:Surface-Active Agents; D053778:Thiazolidines; D011193:Potassium Iodide; D012967:Sodium Dodecyl Sulfate; D011189:Potassium Chloride; D004247:DNA; D003851:Deoxyribonucleases; C037068:latrunculin B