title: Supramolecular effects on the antifungal activity of cyclodextrin/di-n-decyldimethylammonium chloride mixtures.
abstract: Candidiasis infections are growing problem worldwide especially for the immunocompromised individuals. Di-n-decyldimethylammonium chloride is one of the most common antifungal agents used to clean medical devices. The current study examines the antifungal mechanism of di-n-decyldimethylammonium cation and its cyclodextrin inclusion complexes. Depending on the type of cyclodextrin (α-, β- or γ-CD), inclusion complexes can be as active as ammonium alone in terms of microorganism death (fungicidal activity). Moreover, with β-CD inclusion complexes, synergism is observed against fungus growth (fungistatic activity). Based on molecular dynamics, we propose a mechanism supported by cell number, selective electrode and ζ-potential measurements as a function of time. The mechanism involves four steps: (i) the positively-charged complex diffuses through the solution, (ii) it adsorbs onto the fungus membrane surface by electrostatic interaction, (iii) then it dissociates and the ammonium inserts in the microorganism membrane, and (iv) the change of the cell surface charge induces cell lysis.
authors.affiliation: Université Lille 1, EA Chimie Moléculaire et Formulation 4478, Equipe Oxydation et Physico-chimie de Formulation, Bât. C6, F-59655 Villeneuve d'Ascq Cedex, France.
authors.forename: Loïc
authors.initials: L
authors.lastname: Leclercq
authors.forename: Quentin
authors.initials: Q
authors.lastname: Lubart
authors.forename: Anny
authors.initials: A
authors.lastname: Dewilde
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Aubry
authors.forename: Véronique
authors.initials: V
authors.lastname: Nardello-Rataj
mesh_terms: D002176:Candida albicans / Q000187:drug effects* / Q000254:growth & development; D002462:Cell Membrane / Q000187:drug effects; D002626:Chemistry, Pharmaceutical; D003505:Cyclodextrins / Q000737:chemistry*; D004203:Disinfection / Q000379:methods*; D004866:Equipment Contamination / Q000517:prevention & control*; D005659:Fungicides, Industrial / Q000737:chemistry / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D008956:Models, Chemical; D056004:Molecular Dynamics Simulation; D000644:Quaternary Ammonium Compounds / Q000737:chemistry / Q000494:pharmacology*; D055672:Static Electricity; D013678:Technology, Pharmaceutical / Q000379:methods
chemical_list: D003505:Cyclodextrins; D005659:Fungicides, Industrial; D000644:Quaternary Ammonium Compounds; C027118:didecyldimethylammonium