title: Antagonistic action of Bacillus subtilis strain fmbj on the postharvest pathogen Rhizopus stolonifer.
abstract: The antagonistic activities of Bacillus subtilis fmbj against the Rhizopus stolonifer pathogen causing peach soft rot disease were studied in this paper. Bacillus subtilis fmbj exhibited a high antifungal effect on the mycelium growth, sportulation, and germ tube elongation of R. stolonifer with the inhibition rate of 86.5% and 97.42%, respectively, spore germination rate of 20% under antagonist strain concentration of 10⁸ CFU/mL. By using of scanning electron microscope (SEM) and transmission electron microscope (TEM), it was observed that B. subtilis fmbj strain strongly induced morphological abnormalities of R. stolonifer and destroyed structure of hypha and spore. When hypha cell wall of R. stolonifer was damaged, the organelles and cytoplasms inside cell would exude, which led to cell death.
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural Univ., Key Laboratory of Food Processing and Quality Control, Ministry of Agriculture of China, 1 Weigang Nanjing 210095, P.R. China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Zhou
authors.forename: Zhaoxin
authors.initials: Z
authors.lastname: Lu
authors.forename: Fengxia
authors.initials: F
authors.lastname: Lv
authors.forename: Haizhen
authors.initials: H
authors.lastname: Zhao
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Bie
mesh_terms: D001412:Bacillus subtilis / Q000254:growth & development* / Q000378:metabolism*; D061046:Biological Control Agents; D002473:Cell Wall / Q000378:metabolism; D008855:Microscopy, Electron, Scanning / Q000379:methods; D046529:Microscopy, Electron, Transmission / Q000379:methods; D025282:Mycelium / Q000378:metabolism; D027861:Prunus / Q000382:microbiology; D012233:Rhizopus / Q000254:growth & development / Q000472:pathogenicity*; D013172:Spores, Fungal / Q000254:growth & development
chemical_list: D061046:Biological Control Agents