title: Pilot study for the evaluation of morphological and functional changes in retinal blood flow in patients with insulin resistance and/or type 2 diabetes mellitus.
abstract: BACKGROUND
  The aim of this study was to investigate early morphological and functional pathology in the retinal micro-circulation in patients with insulin resistance and/or type 2 diabetes mellitus (T2DM).
  
  
  METHODS
  Fifty-four subjects, without features of retinopathy under ophthalmological investigation, were recruited for study participation and were classified into three study groups according to their metabolic staging: (1) Group C comprised nondiabetic, insulin-sensitive subjects with a BMI <28 kg/m(2); (2) Group IR comprised nondiabetic, insulin-resistant, obese subjects with a BMI ≥28 kg/m(2); and (3) Group DM comprised patients with manifested T2DM. Retinal microvascular blood flow was assessed using scanning laser doppler flowmetry (Heidelberg Retina Flowmeter) before and after flicker light stimulation (10 Hz; Photo Stimulater 750).
  
  
  RESULTS
  No significant difference was observed in retinal blood flow (RBF) among the three groups, neither at baseline nor after stimulating the retina with flicker light. The arterial wall-to-lumen ratio (WLR) tended to be smaller in Group DM compared with Group C, and was significantly lower when comparing Group IR with Group C. When the subjects were grouped according to their insulin resistance, a steady decline in RBF and WLR could be observed with increasing insulin resistance.
  
  
  CONCLUSIONS
  In conclusion, laser scanner flowmetry of the retina was found to detect very early changes in microvascular blood flow. Development of insulin resistance seems to be an important component in the deterioration of RBF.
authors.affiliation: Institute for Clinical Research and Development, Mainz, Germany. thomasf@ikfe.de
authors.forename: Thomas
authors.initials: T
authors.lastname: Forst
authors.forename: Matthias M
authors.initials: MM
authors.lastname: Weber
authors.forename: Michael
authors.initials: M
authors.lastname: Mitry
authors.forename: Thomas
authors.initials: T
authors.lastname: Schöndorf
authors.forename: Senait
authors.initials: S
authors.lastname: Forst
authors.forename: Mukadar
authors.initials: M
authors.lastname: Tanis
authors.forename: Andreas
authors.initials: A
authors.lastname: Pfützner
authors.forename: Georg
authors.initials: G
authors.lastname: Michelson
mesh_terms: D000328:Adult; D000368:Aged; D003430:Cross-Sectional Studies; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000473:pathology* / Q000503:physiopathology; D003930:Diabetic Retinopathy / Q000175:diagnosis / Q000473:pathology; D042241:Early Diagnosis; D005260:Female; D005451:Fluorescein Angiography; D006801:Humans; D007333:Insulin Resistance / Q000502:physiology*; D017078:Laser-Doppler Flowmetry; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000150:complications / Q000503:physiopathology; D010865:Pilot Projects; D012039:Regional Blood Flow; D012164:Retinal Diseases / Q000150:complications / Q000175:diagnosis / Q000473:pathology; D012171:Retinal Vessels / Q000473:pathology* / Q000502:physiology*