title: Lipase-catalyzed interesterification in packed bed reactor using 2 different temperatures.
abstract: Lipase-catalyzed interesterification of high oleic sunflower oil and fully hydrogenated soybean oil (70 : 30, wt/ wt) was carried out in a packed bed reactor using an immobilized lipase from Thermomyces lanuginosus (Lipozyme TL IM) and the effect of a stepwise temperature protocol involving the 2 different temperatures, 60 and 70 °C, was investigated. The melting point of a fat that was incubated at 70 °C for 9 min was 57 °C, which suggested that it should be to employ a lower reaction temperature of 60 °C, after the first 9 min of the reaction. There were no significant differences (P < 0.05) in the conversion degree, triacylglycerol profile, and solid fat content between a constant temperature protocol (70 °C) and a stepwise temperature protocol (a combination of 70 and 60 °C). After 50 cycles, the overall residual activities of enzymes employed in stepwise temperature protocol were significantly (P < 0.05) higher than those of enzymes employed in constant temperature protocol.
authors.affiliation: Dept. of Food & Nutrition, Korea Univ., Seoul, 136-703, Republic of Korea.
authors.forename: Mi-Hwa
authors.initials: MH
authors.lastname: Chae
authors.forename: Hye-Kyung
authors.initials: HK
authors.lastname: Park
authors.forename: Kwang-Il
authors.initials: KI
authors.lastname: Kwon
authors.forename: Jong-Wook
authors.initials: JW
authors.lastname: Kim
authors.forename: Seung In
authors.initials: SI
authors.lastname: Hong
authors.forename: Yangha
authors.initials: Y
authors.lastname: Kim
authors.forename: Byung Hee
authors.initials: BH
authors.lastname: Kim
authors.forename: In-Hwan
authors.initials: IH
authors.lastname: Kim
mesh_terms: D001203:Ascomycota / Q000201:enzymology; D002384:Catalysis; D004800:Enzymes, Immobilized / Q000378:metabolism; D004951:Esterification; D005511:Food Handling / Q000379:methods*; D006865:Hydrogenation; D008049:Lipase / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D019301:Oleic Acid / Q000032:analysis; D010938:Plant Oils / Q000737:chemistry*; D013024:Soybean Oil / Q000737:chemistry*; D000074242:Sunflower Oil; D013696:Temperature; D044366:Transition Temperature; D014280:Triglycerides / Q000032:analysis
chemical_list: D004800:Enzymes, Immobilized; D010938:Plant Oils; D000074242:Sunflower Oil; D014280:Triglycerides; D019301:Oleic Acid; D013024:Soybean Oil; C093666:Lipozyme; D008049:Lipase