title: [Analysis of hepatitis C virus (HCV) subtypes in HIV/HCV co-infected and HCV mono-infected individuals in Guangdong province].
abstract: OBJECTIVE
  To explore the transmission routes, genotypes/subtypes distribution and genetic character of HCV in HIV/HCV co-infected and HCV mono-infected individuals in Guangdong Province.
  
  
  METHODS
  Reverse transcription (RT) nested PCR was performed to amplify the HCV NS5B gene region from 95 HIV/HCV co-infected and 99 HCV mono-infected individuals lived in Guangdong province. The PCR products were then sequenced for HCV subtyping. Genetic analysis was done by MEGA4 software.
  
  
  RESULTS
  (1) HIV/HCV co-infected individuals infected HCV mostly through injection drug use (IDU, 78.9%), the HCV subtypes were identified as 6a (53.7%), 3a (17.9%), 1b (15.8%), 3b (11.6%) and 1a (1.0%) respectively, the genetic distance within subtype 1b was longer than those within other subtypes, the predominant HCV subtype in HIV/HCV co-infected individuals infected through IDU was 6a (60.0%). (2) HCV mono-infected individuals infected HCV mostly through blood or blood products transfusions (80.8%), the HCV subtypes were identified as 1b (67.7%), 6a (17.2%), 3a (6.1%), 2a (5.0%), 3b (2.0%), 4a (1.0%) and 5a (1.0%) respectively, the genetic distance within subtype 1b was also longer than those within other subtypes, the predominant HCV subtype in HCV mono-infected individuals infected through blood or blood products transfusions was 1b (76.2%).
  
  
  CONCLUSIONS
  The diversity of HCV subtypes in HIV/HCV co-infected and HCV mono-infected individuals in Guangdong Province was high, both the major transmission route and HCV subtype between HIV/HCV co-infected individuals and HCV mono-infected individuals were different.
authors.affiliation: Institute of Infectious Diseases, Guangzhou No.8 People's Hospital, Guangzhou, China.
authors.forename: Wei-lie
authors.initials: WL
authors.lastname: Chen
authors.forename: Jing-min
authors.initials: JM
authors.lastname: Nie
authors.forename: Wei-ping
authors.initials: WP
authors.lastname: Cai
authors.forename: Xiao-zhen
authors.initials: XZ
authors.lastname: Yuan
authors.forename: Feng-yu
authors.initials: FY
authors.lastname: Hu
authors.forename: Shao-jing
authors.initials: SJ
authors.lastname: Wei
authors.forename: Yang-bo
authors.initials: YB
authors.lastname: Tang
authors.forename: Fu-chun
authors.initials: FC
authors.lastname: Zhang
authors.forename: Xiao-ping
authors.initials: XP
authors.lastname: Tang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D044466:Asian People; D002681:China / Q000453:epidemiology; D060085:Coinfection / Q000821:virology*; D005260:Female; D005838:Genotype; D006678:HIV; D015658:HIV Infections / Q000453:epidemiology / Q000821:virology*; D016174:Hepacivirus / Q000145:classification / Q000235:genetics*; D006526:Hepatitis C / Q000453:epidemiology / Q000821:virology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010802:Phylogeny; D055815:Young Adult