title: Effect of daily semen centrifugation and resuspension on the longevity of equine sperm quality following cooled storage.
abstract: An experiment was conducted to determine whether cooled semen quality could be maintained for a longer interval by conducting daily centrifugation of extended semen, with resuspension of the sperm pellet in fresh extender. Semen treatments included SP10NC and SP50NC which contained 10 and 50% seminal plasma, respectively, were not centrifuged (NC), and were stored at 4 to 7 °C for 96 h. Treatments SP10C and SP50C contained 10 and 50% seminal plasma, respectively, but were centrifuged (C) after 24, 48, and 72 h of cooled storage, with daily resuspension in fresh extender containing 10% seminal plasma. Percent total sperm motility (TMOT) and progressively motile (PMOT) was reduced (P < 0.05) in the SP50NC treatment after 24, 48, 72, and 96 h of storage, and TMOT did not differ (P > 0.05) in the SP10C, SP50C, SP10NC groups after the same storage periods. The % COMP-(αt) did not differ (P > 0.05) among treatments at any time period. Percent membrane intact sperm (SMI) was reduced in SP50NC, as compared to SP10C at 48, 72, and 96 h (P < 0.05). Daily centrifugation and resuspension of sperm exposed to 50% seminal plasma for the first 24 h (SP50C) yielded similar TMOT, PMOT, VCL, SMI, % COMP-(αt) (P > 0.05) to Groups SP10NC and SP10C after 96 h of storage. Daily centrifugation and resuspension of cool-stored equine semen in fresh extender may be a method to increase sperm longevity.
authors.affiliation: Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Biomedical Sciences, Texas A&M University, College Station, Texas 77843-4475, USA. clove@cvm.tamu.edu
authors.forename: C C
authors.initials: CC
authors.lastname: Love
authors.forename: T L
authors.initials: TL
authors.lastname: Blanchard
authors.forename: D D
authors.initials: DD
authors.lastname: Varner
authors.forename: S P
authors.initials: SP
authors.lastname: Brinsko
authors.forename: J
authors.initials: J
authors.lastname: Voge
authors.forename: S
authors.initials: S
authors.lastname: Bliss
authors.forename: K
authors.initials: K
authors.lastname: Sudderth
authors.forename: S
authors.initials: S
authors.lastname: Teague
authors.forename: K
authors.initials: K
authors.lastname: LaCaze
mesh_terms: D000818:Animals; D002498:Centrifugation / Q000662:veterinary*; D003080:Cold Temperature; D006736:Horses / Q000502:physiology*; D008297:Male; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa / Q000502:physiology*; D013997:Time Factors