title: Inhibition of colony-spreading activity of Staphylococcus aureus by secretion of δ-hemolysin.
abstract: Staphylococcus aureus spreads on the surface of soft agar, a phenomenon we termed "colony spreading." Here, we found that S. aureus culture supernatant inhibited colony spreading. We purified δ-hemolysin (Hld, δ-toxin), a major protein secreted from S. aureus, as a compound that inhibits colony spreading. The culture supernatants of hld-disrupted mutants had 30-fold lower colony-spreading inhibitory activity than those of the parent strain. Furthermore, hld-disrupted mutants had higher colony-spreading ability than the parent strain. These results suggest that S. aureus negatively regulates colony spreading by secreting δ-hemolysin.
authors.affiliation: Laboratory of Microbiology, Graduate School of Pharmaceutical Sciences, University of Tokyo, 3-1, 7-Chome, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Omae
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Sekimizu
authors.forename: Chikara
authors.initials: C
authors.lastname: Kaito
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D017077:Culture Media, Conditioned / Q000737:chemistry / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006460:Hemolysin Proteins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D050296:Microbial Viability / Q000187:drug effects / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D013211:Staphylococcus aureus / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D013997:Time Factors
chemical_list: D001426:Bacterial Proteins; D017077:Culture Media, Conditioned; D006460:Hemolysin Proteins; C025994:delta hemolysin protein, Staphylococcus aureus