title: Preparation of molecular imprinted hydrophobic polymeric nanoparticles having structural memories for lysozyme recognition.
abstract: This study is related to the preparation of lysozyme-imprinted poly(hydroxyethyl methacrylate-N-methacryloyl-(L)-tyroptophan methylester) [nano-MIP] nanoparticles for purification of lysozyme. Nano-MIP particles were found to be 261 nm in diameter with a surface area of 1648 m(2)/g. According to the elemental analysis results, the particles contained 0.85 μmol MATrp/g polymer. The maximum lysozyme adsorption capacity was 1182.8 mg/g. Adsorbed lysozyme was desorbed with 94% recovery. It was observed that after five adsorption-desorption cycles there was no significant loss in adsorption capacity. In order to show the selectivity of the Lys-MIP nanoparticles, adsorption of lysozyme, bovine serum albumin (BSA), and cytochrome c were investigated.
authors.affiliation: Adnan Menderes University, Department of Chemistry, Aydin, Turkey.
authors.forename: M Emin
authors.initials: ME
authors.lastname: Çorman
authors.forename: Sinan
authors.initials: S
authors.lastname: Akgöl
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D045304:Cytochromes c / Q000737:chemistry; D004529:Egg White / Q000737:chemistry; D004655:Emulsions; D006639:Histidine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D007700:Kinetics; D054802:Molecular Imprinting / Q000379:methods*; D009113:Muramidase / Q000737:chemistry* / Q000302:isolation & purification; D053758:Nanoparticles / Q000737:chemistry*; D058105:Polymerization; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry*; D011109:Polymethacrylic Acids / Q000138:chemical synthesis* / Q000737:chemistry*; D012492:Salts / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013379:Substrate Specificity; D013696:Temperature; D014867:Water / Q000737:chemistry
chemical_list: D004655:Emulsions; D011108:Polymers; D011109:Polymethacrylic Acids; D012492:Salts; C579323:poly(hydroxyethyl methacrylate-N-methacryloyl-histidine methyl ester); D014867:Water; D012710:Serum Albumin, Bovine; D006639:Histidine; D045304:Cytochromes c; D009113:Muramidase