title: Early estimation of left ventricular systolic pressure and prediction of successful aortic constriction in a mouse model of pressure overload by ultrasound biomicroscopy.
abstract: Elevation of left ventricular end-systolic pressure (LVESP) and hypertrophic response in mice varies after transverse aorta constriction (TAC). Micromanometric catheterization, conventionally used to select mice with successful TAC, is invasive and nonreusable. We aimed to establish noninvasive imaging protocols for early estimation of successful TAC by ultrasound biomicroscopy (UBM). Out of 55 C57BL/6J mice, we randomly selected 45 as TAC group and 10 as controls. UMB was performed before TAC and, at day 3 and day 14, after TAC. In all mice, LVESP was measured with a Millar conductance catheter at day 14. With LVESP ≥ 150 mm Hg set as indicator of successful TAC (TAC+) and LVESP < 150 mm Hg as unsuccessful (TAC-), receiver operating characteristic curve analysis demonstrated that postoperative inner diameter at aortic banding site (IDb), peak flow velocity at aortic banding site (PVb) and peak flow velocity of right/left common carotid artery (PVr/l) at day 3 served as most effective predictors for LVESP at day 14 (area under curve = 0.9016, 0.9143, 0.8254, respectively. p < 0.01 for all). Among all UBM parameters at day 3, IDb, PVb, right common carotid artery peak flow velocity (PVr) and PVr/l correlated best with LVESP at day 14 (R(2) = 0.5740, 0.6549, 0.5208, 0.2274, respectively. p < 0.01 for all). Furthermore, IDb, PVb, and PVr/l at day 3 most effectively predict long-term cardiac hypertrophy, using the cut-off values of 0.45 mm, 2698.00 mm/s, 3.08, respectively. UBM can be a noninvasive and effective option for early prediction of successful TAC.
authors.affiliation: Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
authors.forename: Jieyun
authors.initials: J
authors.lastname: You
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.forename: Hong
authors.initials: H
authors.lastname: Ma
authors.forename: Jianguo
authors.initials: J
authors.lastname: Jia
authors.forename: Guoliang
authors.initials: G
authors.lastname: Jiang
authors.forename: Zhidan
authors.initials: Z
authors.lastname: Chen
authors.forename: Yong
authors.initials: Y
authors.lastname: Ye
authors.forename: Hui
authors.initials: H
authors.lastname: Gong
authors.forename: Liping
authors.initials: L
authors.lastname: Bu
authors.forename: Junbo
authors.initials: J
authors.lastname: Ge
authors.forename: Yunzeng
authors.initials: Y
authors.lastname: Zou
mesh_terms: D000818:Animals; D001017:Aortic Coarctation / Q000000981:diagnostic imaging* / Q000503:physiopathology; D019540:Area Under Curve; D006332:Cardiomegaly / Q000000981:diagnostic imaging* / Q000503:physiopathology; D016009:Chi-Square Distribution; D004195:Disease Models, Animal; D004452:Echocardiography; D006439:Hemodynamics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D033401:Microscopy, Acoustic / Q000379:methods*; D011237:Predictive Value of Tests; D013599:Systole / Q000502:physiology; D017725:Ventricular Pressure / Q000502:physiology*