title: Effect of dietary Garcinia cambogia extract on serum essential minerals (calcium, phosphorus, magnesium) and trace elements (iron, copper, zinc) in rats fed with high-lipid diet.
abstract: The aim of the study was to investigate the effect of Garcinia cambogia extract on serum calcium (Ca), phosphorus (P), magnesium (Mg), iron (Fe), zinc (Zn) and copper (Cu) concentrations in rats fed with the normal or the high-lipid and -cholesterol diet. Thirty 1-year-old female Sprague-Dawley rats (pathogen-free), weighing an average of 229 g, were randomly assigned to three experimental groups of ten animals each. Diets and tap water were given ad libitum for 75 days. Group 1 (control group) was fed with basal diet (2 % liquid vegetable oil, 0 % cholesterol), while the diets of groups 2 and 3 contained vegetable oil (2 % liquid vegetable oil and 5 % hydrogenated vegetable oil) and cholesterol (3 %) in high levels. 4,5 % G. cambogia extract containing 65 % HCA was added to the diet of group 3 as from day 45. Blood samples were withdrawn on days 0, 45 and 75. Serum mineral levels were analyzed using standard enzymatic colorimetric methods with a spectrophotometer. All significant differences were p<0.05. Serum Ca levels were not significantly different between all groups on days 45 and 75. Serum P level was significantly higher in the group fed with high-lipid diet and G. cambogia extract than in the control group on day 45. Serum Mg level was significantly higher in group 2 than in the control group on day 45. Serum Fe levels were significantly lower in the control group than in the other groups on days 45 and 75. Serum Zn level of the group fed with high-lipid diet and G. cambogia extract was significantly higher than in the control group on day 75. Serum Cu levels were significantly higher in group 2 than in the control group, and in group 3 than in group 2 on day 75. In conclusion, a diet containing the high fat amounts may lead to the increase in circular levels of some minerals due to the short-chain fatty acid production lowering the luminal pH which increases mineral solubility, or serving as a fuel for mucosal cells and stimulating cell proliferation in the large intestine. G. cambogia extract may be used in the P and Cu deficiencies due to increases resulting in the present P and Cu amounts in G. cambogia extract, or the use of phytate P in diet. It was hoped that with further evidence-based study this product will enter to mainstream medicines.
authors.affiliation: Faculty of Veterinary Medicine, Dept. of Biochemistry, Istanbul University, 34320-Avcilar, Istanbul, Turkey. fesen@istanbul.edu.tr
authors.forename: Feraye Esen
authors.initials: FE
authors.lastname: Gürsel
authors.forename: Atila
authors.initials: A
authors.lastname: Ateş
authors.forename: Tanay
authors.initials: T
authors.lastname: Bilal
authors.forename: Ayşen
authors.initials: A
authors.lastname: Altiner
mesh_terms: D000818:Animals; D002118:Calcium / Q000097:blood*; D003300:Copper / Q000097:blood; D059305:Diet, High-Fat / Q000009:adverse effects*; D019587:Dietary Supplements; D005260:Female; D029761:Garcinia cambogia / Q000737:chemistry*; D007501:Iron / Q000097:blood; D008274:Magnesium / Q000097:blood*; D010758:Phosphorus / Q000097:blood*; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014131:Trace Elements / Q000097:blood*; D015032:Zinc / Q000097:blood
chemical_list: D010936:Plant Extracts; D014131:Trace Elements; D010758:Phosphorus; D003300:Copper; D007501:Iron; D008274:Magnesium; D015032:Zinc; D002118:Calcium