title: Selenium inclusion decreases oxidative stress indicators and muscle injuries in sea bass larvae fed high-DHA microdiets.
abstract: The objective of the present study was to determine the effect of Se inclusion in high-DHA and vitamin E microdiets (5 g DHA/100 g dry weight and 300 mg vitamin E/100 g dry weight; 5 g DHA/100 g dry weight and 300 mg vitamin E/100 g dry weight supplemented with Se) in comparison with a control diet (1 g DHA/100 g dry weight and 150 mg vitamin E/100 g dry weight) on sea bass larval growth, survival, biochemical composition, malonaldehyde (MDA) content, muscle morphology and antioxidant enzymes (AOE), insulin-like growth factors (IGF) and myosin expression. For a given DHA and vitamin E dietary content, Se inclusion favoured larval total length and specific growth rate, and reduced the incidence of muscular lesions, MDA contents and AOE gene expression. In contrast, IGF gene expression was elevated in the 5/300 larvae, suggesting an increased muscle mitogenesis that was corroborated by the increase in mRNA copies of myosin heavy chain. The results of the present study denoted the beneficial effect of Se not only in preventing oxidative stress, as a glutathione peroxidase cofactor, but probably due to other as yet unknown physiological functions.
authors.affiliation: Grupo de Investigación en Acuicultura, University of Las Palmas de Gran Canaria, Instituto Universitario de Sanidad Animal, Trasmontaña s/n, 35413 Arucas, Las Palmas, Canary Islands, Spain. monica.betancor102@doctorandos.ulpgc.es
authors.forename: Mónica B
authors.initials: MB
authors.lastname: Betancor
authors.forename: Ma José
authors.initials: MJ
authors.lastname: Caballero
authors.forename: Genciana
authors.initials: G
authors.lastname: Terova
authors.forename: Reda
authors.initials: R
authors.lastname: Saleh
authors.forename: Eyad
authors.initials: E
authors.lastname: Atalah
authors.forename: Tibiábin
authors.initials: T
authors.lastname: Benítez-Santana
authors.forename: J Gordon
authors.initials: JG
authors.lastname: Bell
authors.forename: Marisol
authors.initials: M
authors.lastname: Izquierdo
mesh_terms: D000818:Animals; D017756:Aquaculture; D001492:Bass / Q000378:metabolism; D004032:Diet / Q000662:veterinary*; D004281:Docosahexaenoic Acids / Q000008:administration & dosage*; D005393:Fish Diseases / Q000473:pathology / Q000517:prevention & control; D005979:Glutathione Peroxidase / Q000235:genetics; D007334:Insulin-Like Growth Factor I / Q000235:genetics; D007335:Insulin-Like Growth Factor II / Q000235:genetics; D007814:Larva / Q000737:chemistry / Q000254:growth & development; D008315:Malondialdehyde / Q000032:analysis; D046529:Microscopy, Electron, Transmission; D009132:Muscles / Q000378:metabolism / Q000473:pathology; D018995:Myosin Heavy Chains / Q000235:genetics; D018384:Oxidative Stress / Q000187:drug effects*; D012333:RNA, Messenger / Q000032:analysis; D012643:Selenium / Q000008:administration & dosage* / Q000032:analysis; D013482:Superoxide Dismutase / Q000235:genetics; D014810:Vitamin E / Q000008:administration & dosage
chemical_list: D012333:RNA, Messenger; D014810:Vitamin E; D004281:Docosahexaenoic Acids; D008315:Malondialdehyde; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D018995:Myosin Heavy Chains; D012643:Selenium