title: Acoustic deposition with NIMS as a high-throughput enzyme activity assay.
abstract: Mass spectrometry (MS)-based enzyme assay has been shown to be a useful tool for screening enzymatic activities from environmental samples. Recently, reported approaches for high-specificity multiplexed characterization of enzymatic activities allow for providing detailed information on the range of enzymatic products and monitoring multiple enzymatic reactions. However, the throughput has been limited by the slow liquid-liquid handling and manual analysis. This rapid communication demonstrates the integration of acoustic sample deposition with nanostructure initiator mass spectrometry (NIMS) imaging to provide reproducible measurements of multiple enzymatic reactions at a throughput that is tenfold to 100-fold faster than conventional MS-based enzyme assay. It also provides a simple means for the visualization of multiple reactions and reaction pathways.
authors.affiliation: Nextval, 4186 Sorrento Valley Blvd, Suite G, San Diego, CA 92121, USA.
authors.forename: Matthew
authors.initials: M
authors.lastname: Greving
authors.forename: Xiaoliang
authors.initials: X
authors.lastname: Cheng
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Reindl
authors.forename: Benjamin
authors.initials: B
authors.lastname: Bowen
authors.forename: Kai
authors.initials: K
authors.lastname: Deng
authors.forename: Katherine
authors.initials: K
authors.lastname: Louie
authors.forename: Michael
authors.initials: M
authors.lastname: Nyman
authors.forename: Joseph
authors.initials: J
authors.lastname: Cohen
authors.forename: Anup
authors.initials: A
authors.lastname: Singh
authors.forename: Blake
authors.initials: B
authors.lastname: Simmons
authors.forename: Paul
authors.initials: P
authors.lastname: Adams
authors.forename: Gary
authors.initials: G
authors.lastname: Siuzdak
authors.forename: Trent
authors.initials: T
authors.lastname: Northen
mesh_terms: D000162:Acoustics; D001234:Aspergillus niger / Q000201:enzymology; D001407:Bacillus / Q000201:enzymology; D057075:Enzyme Assays / Q000191:economics / Q000295:instrumentation*; D004867:Equipment Design; D006026:Glycoside Hydrolases / Q000378:metabolism; D057166:High-Throughput Screening Assays / Q000191:economics / Q000295:instrumentation*; D049329:Nanostructures / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000191:economics / Q000295:instrumentation*; D013997:Time Factors; D014995:Xylosidases / Q000378:metabolism
chemical_list: D006026:Glycoside Hydrolases; D014995:Xylosidases