title: A motif of eleven amino acids is a structural adaptation that facilitates motor capability of eutherian prestin.
abstract: Cochlear outer hair cells (OHCs) alter their length in response to transmembrane voltage changes. This so-called electromotility is the result of conformational changes of membrane-bound prestin. Prestin-based OHC motility is thought to be responsible for cochlear amplification, which contributes to the exquisite frequency selectivity and sensitivity of mammalian hearing. Prestin belongs to an anion transporter family, the solute carrier protein 26A (SLC26A). Prestin is unique in this family in that it functions as a voltage-dependent motor protein manifested by two hallmarks, nonlinear capacitance and motility. Evidence suggests that prestin orthologs from zebrafish and chicken are anion exchangers or transporters with no motor function. We identified a segment of 11 amino acid residues in eutherian prestin that is extremely conserved among eutherian species but highly variable among non-mammalian orthologs and SLC26A paralogs. To determine whether this sequence represents a motif that facilitates motor function in eutherian prestin, we utilized a chimeric approach by swapping corresponding residues from the zebrafish and chicken with those of gerbil. Motility and nonlinear capacitance were measured from chimeric prestin-transfected human embryonic kidney 293 cells using a voltage-clamp technique and photodiode-based displacement measurement system. We observed a gain of motor function with both of the hallmarks in the chimeric prestin without loss of transport function. Our results show, for the first time, that the substitution of a span of 11 amino acid residues confers the electrogenic anion transporters of zebrafish and chicken prestins with motor-like function. Thus, this motif represents the structural adaptation that assists gain of motor function in eutherian prestin.
authors.affiliation: Department of Biomedical Sciences, Creighton University School of Medicine, 2500 California Plaza, Omaha, Nebraska 68178, USA.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Tan
authors.forename: Jason L
authors.initials: JL
authors.lastname: Pecka
authors.forename: Jie
authors.initials: J
authors.lastname: Tang
authors.forename: Sándor
authors.initials: S
authors.lastname: Lovas
authors.forename: Kirk W
authors.initials: KW
authors.lastname: Beisel
authors.forename: David Z Z
authors.initials: DZ
authors.lastname: He
mesh_terms: D000222:Adaptation, Physiological / Q000502:physiology*; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000596:Amino Acids; D000818:Animals; D027321:Anion Transport Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D030161:Avian Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D002645:Chickens; D016384:Consensus Sequence; D025802:Electric Capacitance; D019143:Evolution, Molecular; D005561:Formates / Q000378:metabolism; D005849:Gerbillinae; D057809:HEK293 Cells; D006801:Humans; D017136:Ion Transport; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013329:Structure-Activity Relationship; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D027321:Anion Transport Proteins; D030161:Avian Proteins; D005561:Formates; D011993:Recombinant Fusion Proteins; D029961:Zebrafish Proteins; C521130:slc26a5 protein, zebrafish; C030544:formic acid