title: Diallyl sulfide, diallyl disulfide and diallyl trisulfide affect drug resistant gene expression in colo 205 human colon cancer cells in vitro and in vivo.
abstract: To elevate chemo-resistance of human cancer cells is a major obstacle in the treatment and management of malignant cancers. Diallyl sulfide (DAS), diallyl disulfide (DADS) and diallyl trisulfide (DATS) are presented in the Alliaceae family particularly in garlic. Although DAS, DADS and DATS have been shown to exhibit anticancer activities, there is little information on effects of these compounds on drug resistant genes in human colon cancer cells in vitro and in vivo. Herein, we are the first to show that DAS, DADS and DATS at 25 μM for 24-h and 48-h incubations promoted expression of drug resistant genes in colo 205 human colon cancer cells. In vitro experiments indicated that DATS promoted gene expression of multidrug resistant 1 (Mdr1) (p<0.05), and DAS and DADS promoted MRP3 gene expression and DATS alone stimulated gene expression of multidrug resistance-associated protein-1 (MRP1) (p<0.05) in colo 205 cells. In vivo studies demonstrated that DADS and DATS induced Mdr1 and MRP1 gene expression (p<0.05). DADS promoted MRP3 gene expression (p<0.05) as well as DADS and DATS increased MRP4 and MRP6 gene expression (p<0.05) in the colo 205 xenograft mice. Based on our in vitro and in vivo results, diallyl polysulfides (DAS, DADS and DATS) affected the gene expression of the multidrug resistance in colo 205 human colon cancer cells in vitro and in vivo.
authors.affiliation: Department of Surgery, China Medical University Beigang Hospital, Yunlin 651, Taiwan.
authors.forename: Kuang-Chi
authors.initials: KC
authors.lastname: Lai
authors.forename: Chao-Lin
authors.initials: CL
authors.lastname: Kuo
authors.forename: Heng-Chien
authors.initials: HC
authors.lastname: Ho
authors.forename: Jai-Sing
authors.initials: JS
authors.lastname: Yang
authors.forename: Chia-Yu
authors.initials: CY
authors.lastname: Ma
authors.forename: Hsu-Feng
authors.initials: HF
authors.lastname: Lu
authors.forename: Hui-Ying
authors.initials: HY
authors.lastname: Huang
authors.forename: Fu-Shin
authors.initials: FS
authors.lastname: Chueh
authors.forename: Chien-Chih
authors.initials: CC
authors.lastname: Yu
authors.forename: Jing-Gung
authors.initials: JG
authors.lastname: Chung
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics / Q000378:metabolism; D000490:Allium / Q000737:chemistry*; D000498:Allyl Compounds / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology / Q000627:therapeutic use; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D004220:Disulfides / Q000494:pharmacology / Q000627:therapeutic use; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000235:genetics / Q000378:metabolism*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D013440:Sulfides / Q000494:pharmacology / Q000627:therapeutic use*; D023041:Xenograft Model Antitumor Assays
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000498:Allyl Compounds; D000972:Antineoplastic Agents, Phytogenic; D004220:Disulfides; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D010936:Plant Extracts; D013440:Sulfides; C042577:diallyl trisulfide; C028009:diallyl disulfide; C038491:allyl sulfide