title: Ultraviolet Resonance Raman spectroscopy used to study formulations of salmon calcitonin, a starch-peptide conjugate and TGF-β3.
abstract: Ultraviolet Resonance Raman (UVRR) spectroscopy with excitation at 244 nm was investigated here as a possible useful tool for fast characterization of biopharmaceuticals. Studies were performed on three protein drugs: salmon calcitonin (sCT), starch-peptide conjugate, and transforming growth factor-β3 (TGF-β3) adsorbed onto solid granules of tricalcium phosphate (TCP). Secondary structure of sCT was investigated for solutions of 0.5mg/mL up to 200mg/mL, regardless of the turbidity or aggregation states. An increase in β-sheet content was detected when sCT solutions aggregated. UVRR spectroscopy also detected a small amount of residual organic solvent in a starch-peptide conjugate solution containing only 40 μg/mL of peptide. UVRR spectroscopy was then used to characterize a protein, TGF-β3, adsorbed onto solid granules of TCP at 50 and 250 μg/cm(3). This study shows that UVRR is suitable to characterize the protein formulations in a broad range of concentrations, in liquid, aggregated, and solid states.
authors.affiliation: School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Geneva, Switzerland.
authors.forename: E
authors.initials: E
authors.lastname: Patois
authors.forename: I A
authors.initials: IA
authors.lastname: Larmour
authors.forename: S E J
authors.initials: SE
authors.lastname: Bell
authors.forename: C
authors.initials: C
authors.lastname: Palais
authors.forename: M A H
authors.initials: MA
authors.lastname: Capelle
authors.forename: R
authors.initials: R
authors.lastname: Gurny
authors.forename: T
authors.initials: T
authors.lastname: Arvinte
mesh_terms: D002116:Calcitonin / Q000737:chemistry*; D002130:Calcium Phosphates / Q000737:chemistry; D002626:Chemistry, Pharmaceutical / Q000379:methods; D004355:Drug Stability; D010455:Peptides / Q000737:chemistry*; D019999:Pharmaceutical Solutions / Q000737:chemistry*; D017433:Protein Structure, Secondary; D012996:Solutions / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013059:Spectrum Analysis, Raman / Q000379:methods; D013213:Starch / Q000737:chemistry*; D013696:Temperature; D053782:Transforming Growth Factor beta3 / Q000737:chemistry*; D014732:Vibration
chemical_list: D002130:Calcium Phosphates; D010455:Peptides; D019999:Pharmaceutical Solutions; D012996:Solutions; D012997:Solvents; D053782:Transforming Growth Factor beta3; C028815:salmon calcitonin; D013213:Starch; D002116:Calcitonin; C018392:tricalcium phosphate