title: Significant associations of polymorphisms in the prolactin gene with growth traits in Asian seabass (Lates calcarifer).
abstract: Prolactin (encoded by PRL) is a multifunctional hormone involved in osmoregulation, reproduction, growth, development, immunomodulation, endocrine and metabolic regulation. We cloned the full-length cDNA of Asian seabass PRL, searched for polymorphism in the DNA sequence, and conducted association analyses. Twelve SNPs and one 4-bp deletion were identified in PRL. The SNP c.264+127C>G was used for linkage mapping, and this gene was mapped to linkage group 11. The c.264+980_983delTTGT, c.264+127C>G, c.264+138T>G, c.264+269T>C and c.330C>G polymorphisms were genotyped in 521 individuals with growth trait records. Association analyses between single markers and growth traits revealed that the c.264+269T>C SNP was significantly associated with body weight (BW), total length (TL), standard length (SL) and Fulton's condition factor (KTL and KSL), while the other four were not. Analysis of haplotypes showed that there were 10 haplotypes and 22 haplotype combinations in the population. The differences of BW, TL, KTL and KSL among different haplotype combinations were significant.
authors.affiliation: Molecular Population Genetics Group, Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore.
authors.forename: X P
authors.initials: XP
authors.lastname: He
authors.forename: J H
authors.initials: JH
authors.lastname: Xia
authors.forename: C M
authors.initials: CM
authors.lastname: Wang
authors.forename: H Y
authors.initials: HY
authors.lastname: Pang
authors.forename: G H
authors.initials: GH
authors.lastname: Yue
mesh_terms: D000818:Animals; D001492:Bass / Q000235:genetics* / Q000254:growth & development*; D005260:Female; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D008297:Male; D020641:Polymorphism, Single Nucleotide; D011388:Prolactin / Q000235:genetics* / Q000378:metabolism; D017747:Seafood
chemical_list: D029941:Fish Proteins; D011388:Prolactin