title: KLF6SV1 siRNA inhibits proliferation of human lens epithelial cells.
abstract: OBJECTIVE
  To investigate whether transfection with Krüppel-like factor 6 splice variant 1 (KLF6SV1) siRNA can inhibit proliferation of human lens epithelial cell (HLEC).
  
  
  METHODS
  Plasmid containing KLF6SV1 siRNA was used to decrease the level of KLF6SV1 protein in HLEC. The expression of protein27 kinase inhibition protein 1 (p27(kip1)) and proliferation cell nuclear antigen (PCNA) was tested with western blot. Cell proliferation was assayed by 3-(4,5-dimethylthiazolyl-2-)-2,5-diphenyltetrazoliumbromide (MTT) assay and bromodeoxyuridine (BrdU) incorporation.
  
  
  RESULTS
  KLF6SV1 siRNA can decrease KLF6SV1 expression which leads to increased levels of p27(kip1) and decreased expression of PCNA in HLEC. Cells transfected with pKLF6SV1 siRNA showed less viability compared with the control group in vitro.
  
  
  CONCLUSIONS
  KLF6SV1 siRNA can effectively inhibit HLEC proliferation. It can be regarded as a novel target to treat posterior capsular opacity (PCO).
authors.affiliation: Department of Ophthalmology, First clinical college of Harbin Medical University, Harbin, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Su
authors.forename: Yixin
authors.initials: Y
authors.lastname: Qu
authors.forename: Chenggong
authors.initials: C
authors.lastname: Jiang
authors.forename: Lijuan
authors.initials: L
authors.lastname: Liu
authors.forename: Yanchun
authors.initials: Y
authors.lastname: Shan
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
mesh_terms: D049109:Cell Proliferation; D002470:Cell Survival / Q000235:genetics; D002478:Cells, Cultured; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000235:genetics / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D000075723:Kruppel-Like Factor 6; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000378:metabolism*; D007908:Lens, Crystalline / Q000166:cytology* / Q000378:metabolism; D010957:Plasmids; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D014162:Transfection
chemical_list: C436291:CDKN1B protein, human; C105465:KLF6 protein, human; D000075723:Kruppel-Like Factor 6; D051741:Kruppel-Like Transcription Factors; D018809:Proliferating Cell Nuclear Antigen; D011518:Proto-Oncogene Proteins; D034741:RNA, Small Interfering; D050760:Cyclin-Dependent Kinase Inhibitor p27