title: Lower respiratory infections among hospitalized children in New Caledonia: a pilot study for the Pneumonia Etiology Research for Child Health project.
abstract: We conducted a prospective pilot study over a 1-year period in New Caledonia in preparation for the Pneumonia Research for Child Health (PERCH) project. The pathogens associated with hospitalized lower respiratory infections in children were identified through the use of culture of induced sputum and blood, urinary antigen detection, polymerase chain reaction (PCR) on respiratory specimens, and serology on paired sera. Respiratory viruses were detected on respiratory specimens by immunofluorescence and PCR, and by serology on paired sera. Pathogens were detected in 87.9% of the 108 hospitalized cases. Viruses represented 81.6% of the 152 pathogens detected. Respiratory syncytial virus and rhinovirus were the most frequent, accounting for 32.2% and 24.3% of the pathogens identified, respectively. Only 26.3% of 99 induced sputum specimens collected were determined to be of good quality, which may be a consequence of the collection method used.
authors.affiliation: Molecular Epidemiology Laboratory, Institut Pasteur of New Caledonia, BP 61, 98845 Noumea Cedex, New Caledonia. smermond@pasteur.nc
authors.forename: Sylvain
authors.initials: S
authors.lastname: Mermond
authors.forename: Virginie
authors.initials: V
authors.lastname: Zurawski
authors.forename: Eric
authors.initials: E
authors.lastname: D'Ortenzio
authors.forename: Amanda J
authors.initials: AJ
authors.lastname: Driscoll
authors.forename: Andrea N
authors.initials: AN
authors.lastname: DeLuca
authors.forename: Maria
authors.initials: M
authors.lastname: Deloria-Knoll
authors.forename: Jennifer C
authors.initials: JC
authors.lastname: Moïsi
authors.forename: David R
authors.initials: DR
authors.lastname: Murdoch
authors.forename: Isabelle
authors.initials: I
authors.lastname: Missotte
authors.forename: Laurent
authors.initials: L
authors.lastname: Besson-Leaud
authors.forename: Cyril
authors.initials: C
authors.lastname: Chevalier
authors.forename: Vincent
authors.initials: V
authors.lastname: Debarnot
authors.forename: Fabien
authors.initials: F
authors.lastname: Feray
authors.forename: Stephanie
authors.initials: S
authors.lastname: Noireterre
authors.forename: Bernard
authors.initials: B
authors.lastname: Duparc
authors.forename: Frederique
authors.initials: F
authors.lastname: Fresnais
authors.forename: Olivia
authors.initials: O
authors.lastname: O'Connor
authors.forename: Myrielle
authors.initials: M
authors.lastname: Dupont-Rouzeyrol
authors.forename: Orin S
authors.initials: OS
authors.lastname: Levine
mesh_terms: D000293:Adolescent; D000956:Antigens, Viral / Q000652:urine; D001419:Bacteria / Q000302:isolation & purification / Q000472:pathogenicity; D001424:Bacterial Infections / Q000097:blood / Q000453:epidemiology / Q000821:virology; D016022:Case-Control Studies; D002648:Child; D002673:Child, Hospitalized / Q000706:statistics & numerical data*; D002675:Child, Preschool; D005455:Fluorescent Antibody Technique; D006801:Humans; D007223:Infant; D009510:New Caledonia / Q000453:epidemiology; D010850:Picornaviridae Infections / Q000097:blood / Q000453:epidemiology / Q000821:virology; D010865:Pilot Projects; D011014:Pneumonia / Q000209:etiology*; D011446:Prospective Studies; D018357:Respiratory Syncytial Virus Infections / Q000097:blood / Q000453:epidemiology / Q000821:virology; D012136:Respiratory Syncytial Viruses / Q000302:isolation & purification* / Q000472:pathogenicity; D012141:Respiratory Tract Infections / Q000453:epidemiology* / Q000382:microbiology / Q000821:virology; D012229:Rhinovirus / Q000302:isolation & purification / Q000472:pathogenicity; D012698:Serologic Tests; D013048:Specimen Handling / Q000379:methods; D013183:Sputum / Q000382:microbiology / Q000821:virology
chemical_list: D000956:Antigens, Viral