title: Antibacterial activity and mechanism of action of chlorogenic acid.
abstract: In this study, the antibacterial activity and mechanism of action of chlorogenic acid against bacteria were assessed. The data from minimum inhibitory concentration (MIC) values showed that chlorogenic acid effectively inhibited the growth of all tested bacterial pathogens, and the MIC values were ranging from 20 to 80 μg/mL. An investigation into action mode of chlorogenic acid against the pathogen indicated that chlorogenic acid significantly increased the outer and plasma membrane permeability, resulting in the loss of the barrier function, even inducing slight leakage of nucleotide. The leakage of cytoplasmic contents was also observed by electron micrographs. These results supported our hypothesis that chlorogenic acid bound to the outer membrane, disrupted the membrane, exhausted the intracellular potential, and released cytoplasm macromolecules, which led to cell death.
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan Univ., Wuxi 214122, PR China. louzaixiang@126.com
authors.forename: Zaixiang
authors.initials: Z
authors.lastname: Lou
authors.forename: Hongxin
authors.initials: H
authors.lastname: Wang
authors.forename: Song
authors.initials: S
authors.lastname: Zhu
authors.forename: Chaoyang
authors.initials: C
authors.lastname: Ma
authors.forename: Zhouping
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism; D002463:Cell Membrane Permeability / Q000187:drug effects; D002726:Chlorogenic Acid / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000378:metabolism; D005520:Food Preservatives / Q000494:pharmacology*; D005517:Foodborne Diseases / Q000517:prevention & control; D006090:Gram-Negative Bacteria / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D006094:Gram-Positive Bacteria / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D008564:Membrane Potentials / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D046529:Microscopy, Electron, Transmission; D011188:Potassium / Q000378:metabolism; D012329:RNA, Bacterial / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D005520:Food Preservatives; D012329:RNA, Bacterial; D002726:Chlorogenic Acid; D011188:Potassium