title: Proteome-determined type-specific proteins of Mycobacterium avium subspecies paratuberculosis.
abstract: Mycobacterium avium subspecies paratuberculosis (M. a. paratuberculosis) is a pathogen of ruminants, causing paratuberculosis (characterized by severe emaciation). The disease is endemic in many countries including the UK and places a severe economic burden on the global livestock industry. Two types of M. a. paratuberculosis can be classified by pulsed-field electrophoresis (I/III and II), which are phenotypically distinct and appear to have different host preferences. Proteomes of Type I and Type II M. a. paratuberculosis were analyzed by 2-D gel electrophoresis to determine if any significant differences existed between the subtypes. Seven different strains of Type I and 18 strains of Type II were analyzed and compared to detect type-specific differences. These 'type-specific' differences existed regardless of growth phase and were also exhibited in cells isolated directly from pathogenic lesions. Twenty-three spots predominated on the Type I profile, from which 17 proteins were identified. Twenty-one spots predominated on the Type II profile, from which 16 proteins were identified. None of the proteins identified as differentially represented on the profiles of Type I or Type II corresponded to open reading frames of the defining genomic regions as previously described for the Type I (sheep) and Type II (cattle). Sequence polymorphisms existing in Type I and II strains were identified in some open reading frames or regulatory regions of genes that correspond to proteins expressed in a type-specific fashion. The consequence of these is discussed in relation to protein expression and their impact on the type phenotype is discussed.
authors.affiliation: Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik EH26 0PZ, United Kingdom. Val.Hughes@moredun.ac.uk
authors.forename: Valerie
authors.initials: V
authors.lastname: Hughes
authors.forename: Alfredo
authors.initials: A
authors.lastname: Garcia-Sanchez
authors.forename: Stuart
authors.initials: S
authors.lastname: Smith
authors.forename: Kevin
authors.initials: K
authors.lastname: Mclean
authors.forename: Alex
authors.initials: A
authors.lastname: Lainson
authors.forename: Mintu
authors.initials: M
authors.lastname: Nath
authors.forename: Karen
authors.initials: K
authors.lastname: Stevenson
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D015180:Electrophoresis, Gel, Two-Dimensional; D016927:Mycobacterium avium subsp. paratuberculosis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D010283:Paratuberculosis / Q000382:microbiology*; D020543:Proteome / Q000032:analysis* / Q000235:genetics; D040901:Proteomics; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology*; D059467:Transcriptome
chemical_list: D001426:Bacterial Proteins; D020543:Proteome