title: The effects of 17β-estradiol on mitochondrial biogenesis and function in breast cancer cell lines are dependent on the ERα/ERβ ratio.
abstract: OBJECTIVE
  17β-estradiol (E2) is a risk factor for the development of breast cancer, and cause tumorigenesis in epithelial breast cells. Moreover, E2 has distinct effects on different tissues that are attributed to the presence of two estrogen receptor isoforms, ERα and ERβ.
  
  
  METHODS
  The effect of E2 on mitochondrial biogenesis and function was investigated in two breast cancer cell lines with different estrogen receptor ratios, MCF-7 (high ERα/ERβ ratio) and T47D (low ERα/ERβ ratio) cell lines treated with physiological concentrations of E2 (1 nM).
  
  
  RESULTS
  Mitochondria of the MCF-7 cell line showed an increase in proliferation but a decrease in functionality, while the T47D cell line, with low ERα/ERβ ratio, maintained functionality with fewer mitochondria.
  
  
  CONCLUSIONS
  Our results suggest that ERs endowment and its subtypes relation have an effect on treatment response and could contribute new ideas about mitochondria and ERs in breast cancer, as well as new indicators to the disease progression.
authors.affiliation: Grupo multidisciplinar de Oncología Traslacional, Institut Universitari d'Investigació en Ciències de la Salut (IUNICS), Palma de Mallorca, Illes Balears, Spain.
authors.forename: Jorge
authors.initials: J
authors.lastname: Sastre-Serra
authors.forename: Mercedes
authors.initials: M
authors.lastname: Nadal-Serrano
authors.forename: Daniel Gabriel
authors.initials: DG
authors.lastname: Pons
authors.forename: Adamo
authors.initials: A
authors.lastname: Valle
authors.forename: Jordi
authors.initials: J
authors.lastname: Oliver
authors.forename: Pilar
authors.initials: P
authors.lastname: Roca
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D004268:DNA-Binding Proteins / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology*; D047628:Estrogen Receptor alpha / Q000378:metabolism*; D047629:Estrogen Receptor beta / Q000378:metabolism*; D005260:Female; D006801:Humans; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D024101:Mitochondrial Proteins; C499631:TFAM protein, human; D014157:Transcription Factors; D004958:Estradiol; D003576:Electron Transport Complex IV; D000251:Adenosine Triphosphatases