title: Impact of genomic stability on protein expression in endometrioid endometrial cancer.
abstract: BACKGROUND
  Genomic stability is one of the crucial prognostic factors for patients with endometrioid endometrial cancer (EEC). The impact of genomic stability on the tumour tissue proteome of EEC is not yet well established.
  
  
  METHODS
  Tissue lysates of EEC, squamous cervical cancer (SCC), normal endometrium and squamous cervical epithelium were subjected to two-dimensional (2D) gel electrophoresis and identification of proteins by MALDI TOF MS. Expression of selected proteins was analysed in independent samples by immunohistochemistry.
  
  
  RESULTS
  Diploid and aneuploid genomically unstable EEC displayed similar patterns of protein expression. This was in contrast to diploid stable EEC, which displayed a protein expression profile similar to normal endometrium. Approximately 10% of the differentially expressed proteins in EEC were specific for this type of cancer with differential expression of other proteins observed in other types of malignancy (e.g., SCC). Selected proteins differentially expressed in 2D gels of EEC were further analysed in an EEC precursor lesion, that is, atypical hyperplasia of endometrium, and showed increased expression of CLIC1, EIF4A1 and PRDX6 and decreased expression of ENO1, ANXA4, EMD and Ku70.
  
  
  CONCLUSIONS
  Protein expression in diploid and aneuploid genomically unstable EEC is different from the expression profile of proteins in diploid genomically stable EEC. We showed that changes in expression of proteins typical for EEC could already be detected in precursor lesions, that is, atypical hyperplasia of endometrium, highlighting their clinical potential for improving early diagnostics of EEC.
authors.affiliation: Department of Obstetrics and Gynaecology, Karolinska University Hospital, Solna, Karolinska Institute, SE-17176 Stockholm, Sweden. marta.lomnytska@ki.se
authors.forename: M I
authors.initials: MI
authors.lastname: Lomnytska
authors.forename: S
authors.initials: S
authors.lastname: Becker
authors.forename: T
authors.initials: T
authors.lastname: Gemoll
authors.forename: C
authors.initials: C
authors.lastname: Lundgren
authors.forename: J
authors.initials: J
authors.lastname: Habermann
authors.forename: A
authors.initials: A
authors.lastname: Olsson
authors.forename: I
authors.initials: I
authors.lastname: Bodin
authors.forename: U
authors.initials: U
authors.lastname: Engström
authors.forename: U
authors.initials: U
authors.lastname: Hellman
authors.forename: K
authors.initials: K
authors.lastname: Hellman
authors.forename: A-C
authors.initials: AC
authors.lastname: Hellström
authors.forename: S
authors.initials: S
authors.lastname: Andersson
authors.forename: M
authors.initials: M
authors.lastname: Mints
authors.forename: G
authors.initials: G
authors.lastname: Auer
mesh_terms: D018269:Carcinoma, Endometrioid / Q000235:genetics* / Q000378:metabolism; D016889:Endometrial Neoplasms / Q000235:genetics* / Q000378:metabolism; D005260:Female; D042822:Genomic Instability; D006801:Humans; D059467:Transcriptome