title: Ultrasensitive and label-free detection of pathogenic avian influenza DNA by using CMOS impedimetric sensors.
abstract: This work presents miniaturized CMOS (complementary metal oxide semiconductor) sensors for non-faradic impedimetric detection of AIV (avian influenza virus) oligonucleotides. The signal-to-noise ratio is significantly improved by monolithic sensor integration to reduce the effect of parasitic capacitances. The use of sub-μm interdigitated microelectrodes is also beneficial for promoting the signal coupling efficiency. Capacitance changes associated with surface modification, functionalization, and DNA hybridization were extracted from the measured frequency responses based on an equivalent-circuit model. Hybridization of the AIV H5 capture and target DNA probes produced a capacitance reduction of -13.2 ± 2.1% for target DNA concentrations from 1 fM to 10 fM, while a capacitance increase was observed when H5 target DNA was replaced with non-complementary H7 target DNA. With the demonstrated superior sensing capabilities, this miniaturized CMOS sensing platform shows great potential for label-free point-of-care biosensing applications.
authors.affiliation: Institute of Electronics Engineering, National Tsing Hua University, Hsinchu 300, Taiwan, ROC.
authors.forename: Wei-An
authors.initials: WA
authors.lastname: Lai
authors.affiliation: Department of Biological Science and Technology, National Chiao Tung University, Hsinchu 300, Taiwan, ROC.
authors.forename: Chih-Heng
authors.initials: CH
authors.lastname: Lin
authors.affiliation: Department of Biological Science and Technology, National Chiao Tung University, Hsinchu 300, Taiwan, ROC.
authors.forename: Yuh-Shyong
authors.initials: YS
authors.lastname: Yang
authors.affiliation: Department of Electrical Engineering, National Tsing Hua University, Hsinchu 300, Taiwan, ROC; Institute of Electronics Engineering, National Tsing Hua University, Hsinchu 300, Taiwan, ROC; Institute of NanoEngineering and Microsystems, National Tsing Hua University, Hsinchu 300, Taiwan, ROC. Electronic address: sclu@ee.nthu.edu.tw.
authors.forename: Michael S-C
authors.initials: MS
authors.lastname: Lu
mesh_terms: D000818:Animals; D001483:Base Sequence; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D001717:Birds; D015342:DNA Probes / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D055664:Electrochemical Techniques; D004867:Equipment Design; D005453:Fluorescence; D009980:Influenza A virus / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D005585:Influenza in Birds / Q000821:virology*; D009693:Nucleic Acid Hybridization; D045663:Quantum Dots; D012680:Sensitivity and Specificity; D059629:Signal-To-Noise Ratio
chemical_list: D015342:DNA Probes; D004279:DNA, Viral