title: Mapping the corneal sub-basal nerve plexus in orthokeratology lens wear using in vivo laser scanning confocal microscopy.
abstract: OBJECTIVE
  This study was designed to map the sub-basal nerve plexus (SBNP) in the cornea of orthokeratology (OK) lens wearers.
  
  
  METHODS
  Laser scanning confocal microscopy (LSCM) was performed in vivo on three subjects: a non-lens wearer and two OK lens wearers. Scans were performed on the right eye while the left eye fixated a moving target. A total of 575, 430, and 676 contiguous images of the SBNP were taken from the non-lens wearing and the OK lens wearing subjects, respectively, and used to construct maps of the central to mid-peripheral SBNP.
  
  
  RESULTS
  In the non-lens wearing eye, nerves radiated towards a whorl-like complex centered nasally and inferiorly in an overall pattern consistent with previously reported studies. In the OK lens wearing eyes, this whorl pattern was absent, replaced by a tortuous network of nerve fibers centrally, and thicker curvilinear fibers mid-peripherally, particularly in the nasal, inferior, and temporal regions.
  
  
  CONCLUSIONS
  This study maps the corneal SBNP in OK lens wearers and provides compelling evidence that OK lens wear alters the normal SBNP distribution observed in healthy, non-lens wearing eyes.
authors.affiliation: School of Optometry and Vision Science, University of New South Wales, Sydney, Australia.
authors.forename: Edward
authors.initials: E
authors.lastname: Lum
authors.forename: Blanka
authors.initials: B
authors.lastname: Golebiowski
authors.forename: Helen A
authors.initials: HA
authors.lastname: Swarbrick
mesh_terms: D000328:Adult; D003261:Contact Lenses; D003315:Cornea / Q000294:innervation* / Q000502:physiology; D005260:Female; D006801:Humans; D018613:Microscopy, Confocal / Q000379:methods*; D009412:Nerve Fibers / Q000502:physiology; D009415:Nerve Net / Q000033:anatomy & histology*; D009882:Ophthalmic Nerve / Q000033:anatomy & histology* / Q000502:physiology; D054158:Orthokeratologic Procedures / Q000295:instrumentation*; D012016:Reference Values; D012189:Retrospective Studies; D055815:Young Adult