title: IL-9 induces VEGF secretion from human mast cells and IL-9/IL-9 receptor genes are overexpressed in atopic dermatitis.
abstract: Interleukin 9 (IL-9) has been implicated in mast cell-related inflammatory diseases, such as asthma, where vascular endothelial growth factor (VEGF) is involved. Here we report that IL-9 (10-20 ng/ml) induces gene expression and secretion of VEGF from human LAD2. IL-9 does not induce mast cell degranulation or the release of other mediators (IL-1, IL-8, or TNF). VEGF production in response to IL-9 involves STAT-3 activation. The effect is inhibited (about 80%) by the STAT-3 inhibitor, Stattic. Gene-expression of IL-9 and IL-9 receptor is significantly increased in lesional skin areas of atopic dermatitis (AD) patients as compared to normal control skin, while serum IL-9 is not different from controls. These results imply that functional interactions between IL-9 and mast cells leading to VEGF release contribute to the initiation/propagation of the pathogenesis of AD, a skin inflammatory disease.
authors.affiliation: Laboratory of Molecular Immunopharmacology and Drug Discovery, Department of Molecular Physiology and Pharmacology, Tufts University School of Medicine, Boston, Massachusetts, United States of America.
authors.forename: Nikolaos
authors.initials: N
authors.lastname: Sismanopoulos
authors.forename: Danae A
authors.initials: DA
authors.lastname: Delivanis
authors.forename: Konstantinos D
authors.initials: KD
authors.lastname: Alysandratos
authors.forename: Asimenia
authors.initials: A
authors.lastname: Angelidou
authors.forename: Magdalini
authors.initials: M
authors.lastname: Vasiadi
authors.forename: Anastasia
authors.initials: A
authors.lastname: Therianou
authors.forename: Theoharis C
authors.initials: TC
authors.lastname: Theoharides
mesh_terms: D002460:Cell Line; D003876:Dermatitis, Atopic / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D016906:Interleukin-9 / Q000097:blood / Q000235:genetics* / Q000494:pharmacology; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D010766:Phosphorylation; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D053651:Receptors, Interleukin-9 / Q000235:genetics*; D050796:STAT3 Transcription Factor / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: D016906:Interleukin-9; D012333:RNA, Messenger; D053651:Receptors, Interleukin-9; D050796:STAT3 Transcription Factor; D042461:Vascular Endothelial Growth Factor A