title: Suppression of Arabidopsis RING-DUF1117 E3 ubiquitin ligases, AtRDUF1 and AtRDUF2, reduces tolerance to ABA-mediated drought stress.
abstract: Among approximately 480 RING domain-containing E3 Ub ligases in Arabidopsis, three, At3g46620, At5g59550, and At2g39720, have a domain-of-unknown-function (DUF) 1117 motif in their C-terminal regions. At3g46620 and At5g59550 were identified as homologous ABA- and drought-induced RING-DUF1117 genes and were designated AtRDUF1 and AtRDUF2, respectively. Single and double knock-out mutations of AtRDUFs resulted in hyposensitive phenotypes toward ABA in terms of germination rate and stomatal closure and markedly reduced tolerance to drought stress relative to wild-type plants. These results are discussed in the context that AtRDUF1 and AtRDUF2 play combinatorial, but still distinguishable, roles in ABA-mediated dehydration stress responses.
authors.affiliation: Department of Systems Biology, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, Republic of Korea.
authors.forename: Soo Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: Moon Young
authors.initials: MY
authors.lastname: Ryu
authors.forename: Woo Taek
authors.initials: WT
authors.lastname: Kim
mesh_terms: D020816:Amino Acid Motifs; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000502:physiology*; D055864:Droughts; D008969:Molecular Sequence Data; D009154:Mutation; D054829:RING Finger Domains; D013312:Stress, Physiological / Q000235:genetics*; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000502:physiology*
chemical_list: D029681:Arabidopsis Proteins; C572117:RDUF1 protein, Arabidopsis; C572118:RDUF2 protein, Arabidopsis; D044767:Ubiquitin-Protein Ligases