title: Stem cell identification--in vivo lineage analysis versus in vitro isolation and clonal expansion.
abstract: Recently, a dispute has arisen concerning the operational definition and identification of epithelial stem cells. There is a current view which considers that epithelial stem cell identification depends solely on the in vitro isolation and expansion of cells from the putative stem cell. This article argues strongly that this is a perverse and erroneous view, and that lineage labelling, using genetic markers, remains the gold standard for identifying epithelial stem cells and for analysing their behaviour: in vitro methods show, at best, clonogenic potential but not fate, and constitute ancillary support for conclusions drawn from lineage analysis.
authors.affiliation: Digestive Diseases Centre, Blizard Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, UK. n.a.wright@qmul.ac.uk
authors.forename: Nicholas A
authors.initials: NA
authors.lastname: Wright
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D019070:Cell Lineage / Q000235:genetics; D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000378:metabolism*; D005786:Gene Expression Regulation; D005819:Genetic Markers; D006801:Humans; D039902:Multipotent Stem Cells / Q000378:metabolism; D014411:Neoplastic Stem Cells / Q000378:metabolism; D013234:Stem Cells / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D005819:Genetic Markers