title: [A new method for simultaneous assay of six organic acids in Lonicerae Japonicae Flos].
abstract: OBJECTIVE
  To establish a method of quantitative analysis of multi-components by single marker(QAMS) for quality control of Lonicerae Japonicae Flos.
  
  
  METHODS
  The contents of chlorogenic acid(CA) and caffeic acid(CfA) were determined, and the relative correction factors(RCF) of other organic acids were calculated, which were used for the indirect determination.
  
  
  RESULTS
  The RCFs for the neochlorogenic acid(NCA), 3, 4-O-dicaffeoylquinic acid(3, 4-DCA), 3, 5-O-dicaffeoylquinic acid(3, 5-DCA), and 4, 5-O-dicaffeoylquinic acid(4, 5-DCA) were 5.462, 5.689, 2.313, 2.382(to CA) and 3.941, 4.103, 1.669, 1.718(to CfA), respectively. The established method was validated in different laboratories with different high performance liquid chromatography(HPLC) instruments and different chromatographic columns; the result indicated that the reproducibility was satisfactory. There was no significant difference between the established QAMS method and external reference method (P>0.05).
  
  
  CONCLUSIONS
  The established QAMS method can be used for simultaneous determination of 6 organic acids as quality control of Lonicerae Japonicae Flos with easy available standard substances.
authors.affiliation: Institute of Pharmaceutical Informatics, Zhejiang University, Hangzhou 310058, China.
authors.forename: Wen-long
authors.initials: WL
authors.lastname: Li
authors.forename: Wen-ming
authors.initials: WM
authors.lastname: Zhang
authors.forename: Dong-sheng
authors.initials: DS
authors.lastname: Xue
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.forename: Hai-bin
authors.initials: HB
authors.lastname: Qu
mesh_terms: D002109:Caffeic Acids / Q000032:analysis; D002726:Chlorogenic Acid / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D031239:Lonicera / Q000737:chemistry*; D011786:Quality Control; D011801:Quinic Acid / Q000032:analysis; D015203:Reproducibility of Results
chemical_list: D002109:Caffeic Acids; D011801:Quinic Acid; D002726:Chlorogenic Acid