title: Equilibrium and kinetic analysis of human interleukin-13 and IL-13 receptor alpha-2 complex formation.
abstract: Interleukin 13 (IL-13) is a pleiotropic cytokine secreted by activated T cells. Both IL-13 and its polymorphic variant (IL-13-R110Q) have been shown to be associated with multiple diseases such as asthma and allergy. Two IL-13 receptors have been identified, IL-13R alpha-1 receptor (IL-13Rα1) and IL-13R alpha-2 receptor (IL-13Rα2). It has been well established that IL-13 binds to IL-13Rα1 alone with low nM affinity while binding to the IL-13Rα1/IL-4R receptor complex is significantly tighter (pM). The affinity between IL-13 and IL-13Rα2, however, remains elusive. Several values have been reported in the literature varying from 20 pM to 2.5 nM. The affinities previously reported were obtained using surface plasmon resonance (SPR) or Scatchard analysis of (125) I-IL-13 binding data. This report presents the results for the kinetics and equilibrium binding analysis studies performed using label-free kinetic exclusion assay (KEA) for the interaction of human IL-13 and IL-13Rα2. KEA equilibrium analysis showed that the affinities of IL-13Rα2 are 107 and 56 pM for IL-13 and its variant (IL-13-R110Q), respectively. KEA kinetic analysis showed that a tight and very stable complex is formed between IL-13Rα2 and IL-13, as shown by calculated dissociation rate constants slower than 5 × 10(-5) per second. Kinetic analysis also showed significant differences in the kinetic behavior of wild type (wt) versus IL-13-R110Q. IL-13-R110Q not only associates to IL-13Rα2 slower than wt human IL-13 (wt-IL-13), as previously reported, but IL-13-R110Q also dissociates slower than wt-IL-13. These results show that IL-13Rα2 is a high affinity receptor and provide a new perspective on kinetic behavior that could have significant implications in the understanding of the role of IL-13-R110Q in the disease state.
authors.affiliation: Biologics Research, Janssen Research & Development, LLC, Radnor, PA 19087, USA. elacy@its.jnj.com
authors.forename: Eilyn R
authors.initials: ER
authors.lastname: Lacy
mesh_terms: D019943:Amino Acid Substitution; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry; D018793:Interleukin-13 / Q000737:chemistry* / Q000235:genetics; D053663:Interleukin-13 Receptor alpha2 Subunit / Q000737:chemistry* / Q000302:isolation & purification; D007700:Kinetics; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000302:isolation & purification; D020349:Surface Plasmon Resonance
chemical_list: D055767:Immobilized Proteins; D018793:Interleukin-13; D053663:Interleukin-13 Receptor alpha2 Subunit; D011993:Recombinant Fusion Proteins