title: Mass spectrometry-based carboxyl footprinting of proteins: method evaluation.
abstract: Protein structure determines function in biology, and a variety of approaches have been employed to obtain structural information about proteins. Mass spectrometry-based protein footprinting is one fast-growing approach. One labeling-based footprinting approach is the use of a water-soluble carbodiimide, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and glycine ethyl ester (GEE) to modify solvent-accessible carboxyl groups on glutamate (E) and aspartate (D). This paper describes method development of carboxyl-group modification in protein footprinting. The modification protocol was evaluated by using the protein calmodulin as a model. Because carboxyl-group modification is a slow reaction relative to protein folding and unfolding, there is an issue that modifications at certain sites may induce protein unfolding and lead to additional modification at sites that are not solvent-accessible in the wild-type protein. We investigated this possibility by using hydrogen deuterium amide exchange (H/DX). The study demonstrated that application of carboxyl group modification in probing conformational changes in calmodulin induced by Ca(2+) binding provides useful information that is not compromised by modification-induced protein unfolding.
authors.affiliation: Department of Chemistry, Washington University in St. Louis, MO 63130.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Wen
authors.forename: Richard Y-C
authors.initials: RY
authors.lastname: Huang
authors.forename: Robert E
authors.initials: RE
authors.lastname: Blankenship
authors.forename: Michael L
authors.initials: ML
authors.lastname: Gross