title: Study of bi-enzyme immobilization onto layered double hydroxides nanomaterials for histamine biosensor application.
abstract: In this work, we present the development of a hybrid biomembrane based on the immobilization of diamine oxidase (DAO) into LDH thin films for histamine detection. The LDHs preselected as host matrixes are: hydrotalcites (Mg2Al(CO3)0.5(OH)6), lowaite (Mg4Fe(OH)10Cl) and hydrocalumite (Ca2Al(OH)6Cl). The immobilized probes were characterized by atomic force microscopy (AFM) and attenuated total reflection infrared spectroscopy (IR-ATR mode). The analysis of these results shows that the immobilization of DAO occurs with all type of selected LDH and is stable after a 7 day-immersion in phosphate buffer solution. The LDH incorporating magnesium or calcium divalent cations present high-quality surface topology for DAO immobilization and the ability to keep the enzyme in a well conformation for biogenic amines catabolism and histamine detection.
authors.affiliation: nanoBioengineering Group, National Institute of Research and Physicochemical Analysis (INRAP), Biotechpôle de Sidi Thabet, 2020NSidi Thabet, Tunisia.
authors.forename: Z M
authors.initials: ZM
authors.lastname: Baccar
authors.forename: S
authors.initials: S
authors.lastname: Hidouri
authors.forename: A
authors.initials: A
authors.lastname: Errachid
authors.forename: O Ruiz
authors.initials: OR
authors.lastname: Sanchez
mesh_terms: D000536:Aluminum Hydroxide / Q000737:chemistry; D006631:Amine Oxidase (Copper-Containing) / Q000737:chemistry*; D015374:Biosensing Techniques; D002118:Calcium / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006632:Histamine / Q000737:chemistry*; D006878:Hydroxides / Q000737:chemistry*; D008274:Magnesium / Q000737:chemistry; D008276:Magnesium Hydroxide / Q000737:chemistry; D018625:Microscopy, Atomic Force / Q000379:methods; D049329:Nanostructures / Q000737:chemistry*; D011208:Powders / Q000737:chemistry; D013055:Spectrophotometry, Infrared / Q000379:methods; D013499:Surface Properties
chemical_list: D004800:Enzymes, Immobilized; D006878:Hydroxides; D011208:Powders; C010467:hydrotalcite; D000536:Aluminum Hydroxide; D006632:Histamine; D006631:Amine Oxidase (Copper-Containing); D008274:Magnesium; D008276:Magnesium Hydroxide; D002118:Calcium