title: Synthesis and in vitro inhibition properties of siRNA conjugates carrying acridine and quindoline moieties.
abstract: The synthesis of RNA molecules carrying acridine or quindoline residues at their 3'- and 5'-termini is reported. These conjugates are fully characterized by MALDI-TOF mass spectrometry. Modified siRNA duplexes carrying acridine or quindoline moieties were evaluated for inhibition of the tumor necrosis factor. The conjugates showed inhibitory properties similar to those of unmodified RNA duplexes in HeLa cells transfected with oligofectamine. The fluorescent properties of acridine derivatives allow direct observation of the cytoplasmatic distribution of modified siRNA inside the cells.
authors.affiliation: Institute for Research in Biomedicine (IRB Barcelona), IQAC-CSIC, CIBER-BBN Networking Centre on Bioengineering, Biomaterials and Nanomedicine, Baldiri Reixac 10, ES-08028 Barcelona.
authors.forename: Anna
authors.initials: A
authors.lastname: Aviñó
authors.forename: Sandra M
authors.initials: SM
authors.lastname: Ocampo
authors.forename: José C
authors.initials: JC
authors.lastname: Perales
authors.forename: Ramon
authors.initials: R
authors.lastname: Eritja
mesh_terms: D000166:Acridines / Q000737:chemistry*; D000470:Alkaloids / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D007211:Indoles / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D009691:Nucleic Acid Denaturation; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry; D011804:Quinolines / Q000737:chemistry*; D012313:RNA / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014162:Transfection; D000079424:Tumor Necrosis Factor Inhibitors; D048069:Tumor Necrosis Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D000166:Acridines; D000470:Alkaloids; D007211:Indoles; D009841:Oligonucleotides; D011804:Quinolines; D034741:RNA, Small Interfering; D000079424:Tumor Necrosis Factor Inhibitors; D048069:Tumor Necrosis Factors; C016712:quindoline; D012313:RNA