title: A rapid and highly reliable field-based LAMP assay of canine parvovirus.
abstract: Loop-mediated isothermal amplification (LAMP) is known as a rapid and reliable alternative to conventional single-step or nested PCR for detection of genomic DNA of various pathogens in clinical samples. In this study, LAMP assay was developed for canine parvovirus (CPV) and compared with single-step and nested PCR assays. Out of 50 fecal samples from dogs clinically suspected for CPV infections, 19 were found positive by single-step PCR, 22 by nested PCR and 26 by LAMP. LAMP products were subjected to restriction analysis and sequencing to check their specificity. LAMP assay turned out to be a rapid and fairly reproducible method, did not amplify other common canine pathogens and was more sensitive than nested PCR assay. Therefore, it can be regarded as a highly reliable method for routine field diagnosis of CPV infection.
  
  
  BACKGROUND
  canine parvovirus; nested polymerase chain reaction; loop-mediated isothermal amplification; sensitivity; specificity.
authors.affiliation: Department of Animal Biotechnology, Madras Veterinary College, Chennai, India. drparthiban66@gmail.com
authors.forename: M
authors.initials: M
authors.lastname: Parthiban
authors.forename: K C
authors.initials: KC
authors.lastname: Divya
authors.forename: K
authors.initials: K
authors.lastname: Kumanan
authors.forename: D S
authors.initials: DS
authors.lastname: Bargavi
mesh_terms: D000818:Animals; D052160:Benzothiazoles; D003959:Diamines; D004285:Dogs; D005243:Feces / Q000821:virology; D021141:Nucleic Acid Amplification Techniques / Q000379:methods / Q000662:veterinary*; D009930:Organic Chemicals; D010322:Parvoviridae Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D017993:Parvovirus, Canine / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D011804:Quinolines; D012680:Sensitivity and Specificity
chemical_list: D052160:Benzothiazoles; D003959:Diamines; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I