title: 16S ribosomal DNA clone libraries to reveal bacterial diversity in anaerobic reactor-degraded tetrabromobisphenol A.
abstract: Microorganisms able to rapidly degrade tetrabromobisphenol A (TBBPA) were domesticated in an anaerobic reactor and added to gradually increased concentrations of TBBPA. After 240 days of domestication, the degradation rate reached 96.0% in cultivated batch experiments lasting 20 days. The optimum cultivating temperature and pH were 30°C and 7.0. The bacterial community's composition and diversity in the reactor was studied by comparative analysis with 16S ribosomal DNA clone libraries. Amplified rDNA restriction analysis of 200 clones from the library indicate that the rDNA richness was high (Coverage C 99.5%) and that evenness was not high (Shannon-Weaver index 2.42). Phylogenetic analysis of 63 bacterial sequences from the reactor libraries demonstrated the presence of Betaproteobacteria (33.1%), Gammaproteobacteria (18.7%), Bacteroidetes (13.9%), Firmicutes (11.4%), Chloroflexi (3.6%), Actinobacteria (0.6%), the candidate division TM7 (4.2%) and other unknown, uncultured bacterial groups (14.5%). Comamonas, Achromobacter, Pseudomonas and Flavobacterium were the dominant types.
authors.affiliation: School of Environmental Science and Engineering, Sun Yat-Sen University, Guangzhou 510275, China.
authors.forename: Xingxing
authors.initials: X
authors.lastname: Peng
authors.forename: Zaili
authors.initials: Z
authors.lastname: Zhang
authors.forename: Ziling
authors.initials: Z
authors.lastname: Zhao
authors.forename: Xiaoshan
authors.initials: X
authors.lastname: Jia
mesh_terms: D000693:Anaerobiosis; D001419:Bacteria / Q000235:genetics*; D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology*; D004275:DNA, Ribosomal / Q000235:genetics*; D015723:Gene Library; D014644:Genetic Variation; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010802:Phylogeny; D011075:Polybrominated Biphenyls / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013696:Temperature
chemical_list: D004275:DNA, Ribosomal; D011075:Polybrominated Biphenyls; C020806:tetrabromobisphenol A