title: New generation QuIC assays for prion seeding activity.
abstract: The ability of abnormal TSE-associated forms of PrP to seed the formation of amyloid fibrils from recombinant PrP(Sen) has served as the basis for several relatively rapid and highly sensitive tests for prion diseases. These tests include rPrP-PMCA (rPMCA), standard quaking-induced conversion (S-QuIC), amyloid seeding assay (ASA), real-time QuIC (RT-QuIC) and enhanced QuIC (eQuIC). Here, we summarize recent improvements in the RT-QuIC-based assays that enhance the practicality, sensitivity and quantitative attributes of assays QuIC and promote the detection of prion seeding activity in dilute, inhibitor-laden fluids such as blood plasma.
authors.affiliation: Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA.
authors.forename: Christina D
authors.initials: CD
authors.lastname: Orrù
authors.forename: Jason M
authors.initials: JM
authors.lastname: Wilham
authors.forename: Sarah
authors.initials: S
authors.lastname: Vascellari
authors.forename: Andrew G
authors.initials: AG
authors.lastname: Hughson
authors.forename: Byron
authors.initials: B
authors.lastname: Caughey
mesh_terms: D000682:Amyloid / Q000032:analysis / Q000378:metabolism; D000818:Animals; D001681:Biological Assay / Q000379:methods*; D001923:Brain Chemistry; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D011328:Prions / Q000032:analysis* / Q000097:blood / Q000737:chemistry / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D000682:Amyloid; D011328:Prions; D011994:Recombinant Proteins