title: Reducing the inhibitory effect of cigarette smoke on the activity of oral peroxidase by the addition of berberine in cigarette filter.
abstract: This study was carried out to evaluate the effect of cigarette smoke (CS) on the activity of oral peroxidase (OPO) after berberine was added to the cigarette filter. Activated carbon fiber (ACF) was chosen to load berberine as a part of the cellulose acetate (CA) filter to obtain the modified B-ACF cigarette filter. Then the effects of CS from the testing cigarettes on the activity of OPO were investigated in vitro by the 2-nitrobenzoic acid assay, and the smoke chemistry was also analyzed, especially the content of hydrogen cyanide (HCN) in the CS. The results indicated that the loss of activity of OPO in B-ACF filter cigarette group decreased by 20% and 25%, compared with those of ACF and CA filter cigarette groups, respectively. The relative residual activity of OPO in B-ACF filter group was increased with the increase of berberine in the filter compared with the CA filter group. It could be observed that the reduction in HCN might be related to the berberine in the cigarette filter, reducing the inhibition of CS on the activity of OPO.
authors.affiliation: Chemistry Institute of Pharmaceutical Resources, School of Pharmaceutical Science, Southwest University, Chongqing, China.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Wu
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Ye
authors.forename: Xuelong
authors.initials: X
authors.lastname: Cui
authors.forename: Xuegang
authors.initials: X
authors.lastname: Li
authors.forename: Lifeng
authors.initials: L
authors.lastname: Zheng
authors.forename: Zhu
authors.initials: Z
authors.lastname: Chen
mesh_terms: D001599:Berberine / Q000494:pharmacology*; D006801:Humans; D009195:Peroxidase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D012463:Saliva / Q000187:drug effects* / Q000201:enzymology*; D012906:Smoke / Q000032:analysis; D012907:Smoking / Q000378:metabolism*; D062789:Tobacco Products
chemical_list: D012906:Smoke; D001599:Berberine; D009195:Peroxidase