title: Cytotoxicity of water-soluble mPEG-SH-coated silver nanoparticles in HL-7702 cells.
abstract: Silver nanoparticles (AgNPs) are being used widely and increasingly in various products and medical supplies due to their antibacterial activity. However, little is known about the impacts of the AgNPs. Herein, The primary purpose of this study was to investigate the cytotoxic effect of AgNPs in the human liver cell line (HL-7702). The water-soluble α-Methoxy-poly (ethylene glycol)-ω-mercapto (mPEG-SH)-coated AgNPs (40 nm) were synthesized, which showed superior stabilization and uniform dispersion in culture medium. The effect of mPEG-SH-coated silver nanoparticles on cell viability, leakage of lactate dehydrogenase (LDH), oxidative stress, mitochondrial membrane potential (MMP), and cell cycle was evaluated after the cells were treated with nanoparticles. The results showed that the coated AgNPs could be taken up by cells, decreased cell viability in dose- and time-dependent manners at dosage levels between 6.25 and 100.00 μg/mL, caused membrane damage (LDH leakage), and decreased the activities of superoxide dismutase and glutathione peroxides. The level of malondialdehyde, an end product of lipid peroxidation, was also increased in AgNPs-exposed cells. Moreover, flow cytometric analysis showed that AgNP exposure decrease MMP and cause G₂/M phase arrest. Thus, our data suggest that mPEG-SH-coated AgNPs have the potential toxicity that is associated with oxidative stress, apoptosis, and DNA damage.
authors.affiliation: Department of Toxicology, School of Public Health, Jilin University, Changchun 130021, People's Republic of China.
authors.forename: Xiu-ling
authors.initials: XL
authors.lastname: Song
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.forename: Kun
authors.initials: K
authors.lastname: Xu
authors.forename: Juan
authors.initials: J
authors.lastname: Liu
authors.forename: Wen
authors.initials: W
authors.lastname: Ju
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.forename: Xiao-dong
authors.initials: XD
authors.lastname: Liu
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.forename: Yan-fei
authors.initials: YF
authors.lastname: Qi
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002626:Chemistry, Pharmaceutical; D004305:Dose-Response Relationship, Drug; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D053768:Metal Nanoparticles / Q000633:toxicity* / Q000648:ultrastructure; D018384:Oxidative Stress / Q000187:drug effects; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry*; D012834:Silver / Q000378:metabolism / Q000633:toxicity*; D012995:Solubility; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D013438:Sulfhydryl Compounds; D012834:Silver; D011092:Polyethylene Glycols; D008315:Malondialdehyde; C028210:monomethoxypolyethylene glycol; D007770:L-Lactate Dehydrogenase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase