title: Biomechanics of the human posterior sclera: age- and glaucoma-related changes measured using inflation testing.
abstract: OBJECTIVE
  The objective of this study was to measure the biomechanical response of the human posterior sclera in vitro and to estimate the effects of age and glaucoma.
  
  
  METHODS
  Scleral specimens from 22 donors with no history of glaucoma and 11 donors with a history of glaucoma were excised 3 mm posterior to the equator and affixed to an inflation chamber. Optic nerve cross-sections were graded to determine the presence of axon loss. The time-dependent inflation response was measured in a series of pressure-controlled load-unload tests to 30 mm Hg and creep tests to 15 and 30 mm Hg. Circumferential and meridional strains were computed from the digital image correlation displacements, and midposterior stresses were determined from pressure and deformed geometry.
  
  
  RESULTS
  Among normal specimens, older age was predictive of a stiffer response and a thinner sclera. In the age group 75 to 93, diagnosed glaucoma eyes with axon damage were thicker than normal eyes. Both damaged and undamaged glaucoma eyes had a different strain response in the peripapillary sclera characterized by a stiffer meridional response. Undamaged glaucoma eyes had slower circumferential creep rates in the peripapillary sclera than normal eyes. Glaucoma eyes were not different from normal eyes in stresses and strains in the midposterior sclera.
  
  
  CONCLUSIONS
  The observed differences in the biomechanical response of normal and glaucoma sclera may represent baseline properties that contribute to axon damage, or may be characteristics that result from glaucomatous disease.
authors.affiliation: Department of Mechanical Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.
authors.forename: Baptiste
authors.initials: B
authors.lastname: Coudrillier
authors.forename: Jing
authors.initials: J
authors.lastname: Tian
authors.forename: Stephen
authors.initials: S
authors.lastname: Alexander
authors.forename: Kristin M
authors.initials: KM
authors.lastname: Myers
authors.forename: Harry A
authors.initials: HA
authors.lastname: Quigley
authors.forename: Thao D
authors.initials: TD
authors.lastname: Nguyen
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000502:physiology*; D001696:Biomechanical Phenomena / Q000502:physiology; D003941:Diagnostic Techniques, Ophthalmological; D004548:Elasticity / Q000502:physiology*; D005109:Extracellular Matrix / Q000502:physiology; D005260:Female; D005901:Glaucoma / Q000503:physiopathology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D057972:Posterior Eye Segment / Q000502:physiology*; D012590:Sclera / Q000503:physiopathology*; D013997:Time Factors