title: Early B-cell factors 2 and 3 (EBF2/3) regulate early migration of Cajal-Retzius cells from the cortical hem.
abstract: Cajal-Retzius (CR) cells play a crucial role in the formation of the cerebral cortex, yet the molecules that control their development are largely unknown. Here, we show that Ebf transcription factors are expressed in forebrain signalling centres-the septum, cortical hem and the pallial-subpallial boundary-known to generate CR cells. We identified Ebf2, through fate mapping studies, as a novel marker for cortical hem- and septum-derived CR cells. Loss of Ebf2 in vivo causes a transient decrease in CR cell numbers on the cortical surface due to a migratory defect in the cortical hem, and is accompanied by upregulation of Ebf3 in this and other forebrain territories that produce CR cells, without affecting proper cortical lamination. Accordingly, using in vitro preparations, we demonstrated that both Ebf2 and Ebf3, singly or together, control the migration of CR cells arising in the cortical hem. These findings provide evidence that Ebfs directly regulate CR cell development.
authors.affiliation: Department of Cell and Developmental Biology, University College London, UK.
authors.forename: Francesca
authors.initials: F
authors.lastname: Chiara
authors.forename: Aurora
authors.initials: A
authors.lastname: Badaloni
authors.forename: Laura
authors.initials: L
authors.lastname: Croci
authors.forename: Mason L
authors.initials: ML
authors.lastname: Yeh
authors.forename: Anna
authors.initials: A
authors.lastname: Cariboni
authors.forename: Anna
authors.initials: A
authors.lastname: Hoerder-Suabedissen
authors.forename: G Giacomo
authors.initials: GG
authors.lastname: Consalez
authors.forename: Britta
authors.initials: B
authors.lastname: Eickholt
authors.forename: Tomomi
authors.initials: T
authors.lastname: Shimogori
authors.forename: John G
authors.initials: JG
authors.lastname: Parnavelas
authors.forename: Sonja
authors.initials: S
authors.lastname: Rakić
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000502:physiology*; D002454:Cell Differentiation; D019070:Cell Lineage; D002465:Cell Movement / Q000502:physiology; D002540:Cerebral Cortex / Q000166:cytology / Q000196:embryology*; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000502:physiology; D014157:Transcription Factors / Q000502:physiology*
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D014157:Transcription Factors; C489922:Ebf2 protein, mouse; C110117:Ebf3 protein, mouse