title: A case study of human papillomavirus-associated bladder carcinoma developing after urethral condyloma acuminatum.
abstract: We report a 35-year-old man with bladder carcinoma developing 2 months after urethral condyloma acuminatum, with an 8-year history of persistent human papilloma virus-58 infection in the urinary tract. DNA was extracted from paraffin-embedded tissue specimens. Human papilloma virus-6 and -58 were detected in the condyloma, whereas human papilloma virus-58 was detected in the carcinoma. In situ hybridization analysis also demonstrated high-risk human papilloma virus-DNA signals in the condyloma and carcinoma tissues. Immunohistochemistry showed that p16-INK4a and mcm-7, surrogate markers of oncogenic human papilloma virus E7 protein, were weakly expressed in the condyloma tissue but were strongly expressed in the carcinoma tissues, suggesting that human papilloma virus-58 was present in the episomal state in the condyloma, whereas human papilloma virus-58 DNA was integrated into the host cells and its infection may have a role in the development of bladder carcinoma. Human papilloma virus-58 was continuously detected in the urethral brushing samples 8 years after treatment for urethral condyloma, and human papilloma virus-58 infection was still persistent in the urethra.
authors.affiliation: Department of Integrative Cancer Therapy and Urology, Kanazawa University Graduate School of Medical Science, 13-1, Takaramachi, Kanazawa, Ishikawa 920-8641, Japan.
authors.forename: Shohei
authors.initials: S
authors.lastname: Kawaguchi
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Shigehara
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Sasagawa
authors.forename: Masato
authors.initials: M
authors.lastname: Kuribayashi
authors.forename: Akira
authors.initials: A
authors.lastname: Junicho
authors.forename: Toru
authors.initials: T
authors.lastname: Hasegawa
authors.forename: Yuji
authors.initials: Y
authors.lastname: Maeda
authors.forename: Mikio
authors.initials: M
authors.lastname: Namiki
mesh_terms: D000328:Adult; D052685:Alphapapillomavirus; D002277:Carcinoma / Q000821:virology*; D003218:Condylomata Acuminata / Q000150:complications* / Q000821:virology; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D014522:Urethral Diseases / Q000150:complications* / Q000821:virology; D001749:Urinary Bladder Neoplasms / Q000821:virology*