title: Assessing the validity of an ELISA test for the serological diagnosis of human fascioliasis in different epidemiological situations.
abstract: OBJECTIVE
  To improve the diagnosis of human fascioliasis caused by Fasciola hepatica and Fasciola gigantica, we evaluated the diagnostic accuracy of an enzyme-linked immunosorbent assay (ELISA), with Fasciola antigen from the adult liver fluke, for the detection of IgG against fascioliasis in human sera.
  
  
  METHODS
  The sera of 54 fascioliasis cases, originating from three endemic areas, were used in this evaluation: (i) a hyperendemic F. hepatica area where humans usually shed a great number of parasite eggs in faeces (11 sera); (ii) an epidemic F. hepatica area where humans usually shed small amounts of parasite eggs (24 sera) and (iii) an overlap area of both Fasciola species and where human shedding of parasite eggs in faeces is usually scarce or non-existent (19 sera). One hundred and sixty-eight patients with other parasitic infections and 89 healthy controls were also analysed.
  
  
  RESULTS
  The respective sensitivity and specificity of this assay were 95.3% (95% confidence intervals, 82.9-99.2%) and 95.7% (95% confidence intervals, 92.3-97.5%). No correlation between egg output and the OD450 values of the F. hepatica IgG ELISA test was observed.
  
  
  CONCLUSIONS
  This test could be used both as an individual serodiagnostic test for human fascioliasis when backed up by a compatible clinical history together with a second diagnostic technique for other cross-reactive helminth infections, and in large-scale epidemiological studies of human fascioliasis worldwide.
authors.affiliation: Departamento de Parasitología, Facultad de Farmacia, Universidad de Valencia, Spain. madela.valero@uv.es
authors.forename: M Adela
authors.initials: MA
authors.lastname: Valero
authors.forename: M Victoria
authors.initials: MV
authors.lastname: Periago
authors.forename: Ignacio
authors.initials: I
authors.lastname: Pérez-Crespo
authors.forename: Esperanza
authors.initials: E
authors.lastname: Rodríguez
authors.forename: M Jesús
authors.initials: MJ
authors.lastname: Perteguer
authors.forename: Teresa
authors.initials: T
authors.lastname: Gárate
authors.forename: Eva M
authors.initials: EM
authors.lastname: González-Barberá
authors.forename: Santiago
authors.initials: S
authors.lastname: Mas-Coma
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D003937:Diagnosis, Differential; D019353:Endemic Diseases / Q000706:statistics & numerical data; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards*; D005211:Fascioliasis / Q000097:blood* / Q000175:diagnosis* / Q000453:epidemiology; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007223:Infant; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000592:standards*; D013030:Spain / Q000453:epidemiology; D055815:Young Adult
chemical_list: D007074:Immunoglobulin G