title: Development and characterisation of chitosan films impregnated with insulin loaded PEG-b-PLA nanoparticles (NPs): a potential approach for buccal delivery of macromolecules.
abstract: Mucoadhesive chitosan based films, incorporated with insulin loaded nanoparticles (NPs) made of poly(ethylene glycol)methyl ether-block-polylactide (PEG-b-PLA) have been developed and characterised. Blank-NPs were prepared by double emulsion solvent evaporation technique with varying concentrations of the copolymer (5 and 10%, w/v). The optimised formulation was loaded with insulin (model protein) at initial loadings of 2, 5 and 10% with respect to copolymer weight. The developed NPs were analysed for size, size distribution, surface charge, morphology, encapsulation efficiency and drug release. NPs showing negative (ζ)-potential (<-6 mV) with average diameter> 300 nm and a polydispersity index (P.I.) of ≈ 0.2, irrespective of formulation process, were achieved. Insulin encapsulation efficiencies of 70% and 30% for NPs-Insulin-2 and NPs-Insulin-5 were obtained, respectively. The in vitro release behaviour of both formulations showed a classic biphasic sustained release of protein over 5 weeks which was influenced by pH of the release medium. Optimised chitosan films embedded with 3mg of insulin loaded NPs were produced by solvent casting with homogeneous distribution of NPs in the mucoadhesive matrix, which displayed excellent physico-mechanical properties. The drug delivery system has been designed as a novel platform for potential buccal delivery of macromolecules.
authors.affiliation: Department of Pharmaceutical, Chemical and Environmental Sciences, School of Science, University of Greenwich at Medway, Chatham Maritime, Kent ME4 4TB, UK.
authors.forename: Concetta
authors.initials: C
authors.lastname: Giovino
authors.forename: Isaac
authors.initials: I
authors.lastname: Ayensu
authors.forename: John
authors.initials: J
authors.lastname: Tetteh
authors.forename: Joshua S
authors.initials: JS
authors.lastname: Boateng
mesh_terms: D000278:Administration, Buccal; D002626:Chemistry, Pharmaceutical / Q000379:methods; D048271:Chitosan / Q000008:administration & dosage / Q000737:chemistry*; D004304:Dosage Forms; D004337:Drug Carriers / Q000008:administration & dosage / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods*; D004655:Emulsions / Q000008:administration & dosage / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007328:Insulin / Q000008:administration & dosage / Q000737:chemistry*; D007773:Lactates / Q000008:administration & dosage / Q000737:chemistry*; D046911:Macromolecular Substances / Q000008:administration & dosage / Q000737:chemistry*; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D010316:Particle Size; D011092:Polyethylene Glycols / Q000008:administration & dosage / Q000737:chemistry*; D014014:Tissue Adhesives / Q000378:metabolism
chemical_list: D004304:Dosage Forms; D004337:Drug Carriers; D004655:Emulsions; D007328:Insulin; D007773:Lactates; D046911:Macromolecular Substances; D014014:Tissue Adhesives; C088513:poly(lactic acid-ethylene glycol); D011092:Polyethylene Glycols; D048271:Chitosan