title: A miniature probe for ultrasonic penetration of a single cell.
abstract: Although ultrasound cavitation must be avoided for safe diagnostic applications, the ability of ultrasound to disrupt cell membranes has taken on increasing significance as a method to facilitate drug and gene delivery. A new ultrasonic resonance driving method is introduced to penetrate rigid wall plant cells or oocytes with springy cell membranes. When a reasonable design is created, ultrasound can gather energy and increase the amplitude factor. Ultrasonic penetration enables exogenous materials to enter cells without damaging them by utilizing instant acceleration. This paper seeks to develop a miniature ultrasonic probe experiment system for cell penetration. A miniature ultrasonic probe is designed and optimized using the Precise Four Terminal Network Method and Finite Element Method (FEM) and an ultrasonic generator to drive the probe is designed. The system was able to successfully puncture a single fish cell.
authors.affiliation: MEMS Lab, Department of Precision Instruments & Mechanology, Tsinghua University, Beijing 100084, P.R. China; E-Mails:  zhouzy@mail.tsinghua.edu.cn ;  yangxing@mail.tsinghua.edu.cn ;  xiaomf03@mails.tsinghua.edu.cn.
authors.forename: Ting
authors.initials: T
authors.lastname: Wu
authors.forename: Zhaoying
authors.initials: Z
authors.lastname: Zhou
authors.forename: Qun
authors.initials: Q
authors.lastname: Wang
authors.forename: Xing
authors.initials: X
authors.lastname: Yang
authors.forename: Mingfei
authors.initials: M
authors.lastname: Xiao