title: [Effects and mechanisms of autophagy of trophoblast cells in severe preeclampsia].
abstract: OBJECTIVE
  To investigate the occurrence law of autophagy in trophoblast cells from severe preeclampsia and its possible mechanisms of wrecking cells.
  
  
  METHODS
  23 cases of placenta tissues were collected from women suffer from severe preeclampsia and normal pregnant women respectively. Surface villous of placental was observed by scanning electron microscope,  and ultrastructure and autophagosome of trophoblast cells were observed by transmission electron microscopic. The expressions of Beclin-1 and LC3II in placenta tissues were detected by Immunohistochemical technique,  then its relevance whit weight of fetus and placental was analyzed.
  
  
  RESULTS
  Compared with the control group,  villous were decrease in placenta tissue from patients suffer from severe preeclampsia,  and its arrangement was disordered;  typical autophagosomes of trophoblast cells were observed by transmission electron microscope. The expression of Beclin-1,  LC3IIwere significantly up-regulated in placenta tissue from patients suffer from severe preeclampsia.The correlation coefficient of Beclin-1,  LC3II and placental weight,  fetal weight respectively were -0.977,  -0.930,  -0.812,  -0.849.
  
  
  CONCLUSIONS
  Autophagy were significantly up-regulated in trophoblast cells from patients suffer from severe preeclampsia by increasing expression of Beclin-1,  LC3II protein,  and its expression was negatively related to weight of fetus and placental.
authors.affiliation: Department of Obstetrics and Gynecology, Chongqing Medical University, Chongqing, China. cgq19841001@126.com
authors.forename: Guo-qing
authors.initials: GQ
authors.lastname: Chen
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.forename: Hong-bo
authors.initials: HB
authors.lastname: Qi
authors.forename: Zhen-wei
authors.initials: ZW
authors.lastname: Yao
authors.forename: Li
authors.initials: L
authors.lastname: Gao
authors.forename: Chun-ling
authors.initials: CL
authors.lastname: Qiu
mesh_terms: D000328:Adult; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D001343:Autophagy; D000071186:Beclin-1; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D010920:Placenta / Q000378:metabolism; D011225:Pre-Eclampsia / Q000378:metabolism / Q000473:pathology*; D011247:Pregnancy; D014327:Trophoblasts / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D055815:Young Adult
chemical_list: D051017:Apoptosis Regulatory Proteins; C491997:BECN1 protein, human; D000071186:Beclin-1; C477012:MAP1LC3A protein, human; D008565:Membrane Proteins; D008869:Microtubule-Associated Proteins