title: Preparation of a new nanosized As2O3/Mn0.5Zn0.5Fe2O4 thermosensitive magnetoliposome and its antitumor effect on MDA_MB_231 cells.
abstract: The purpose of our research is to explore the preparation method of a new nanosized As2O3/Mn0.5Zn0.5Fe2O4 thermosensitive magnetoliposome and study its antitumor effect on MDA_MB_231 cells. The liposomes prepared by the method of rotatory film and high-pressure homogenization were detected by transmission electron microscopy (TEM), scanning electron microscopy (SEM), energy dispersive spectrometry (EDS), nano-particles detector, atom fluorescence spectrophotometer and differential scanning calorimetry (DSC). The therapeutic effects of the nanosized thermosensitive magnetoliposomes combined hyperthermia on human MDA_MB_231 cells in vitro were evaluated by MTT assay and flow cytometry assay. The results indicated that the nanosized As2O3/Mn0.5Zn0.5Fe2O4 thermosensitive magnetoliposomes were prepared successfully. The liposomes were spherical, and most of them were single-room. The exat average diameter of them was 103.8 nm. EDS showed each nanosized As2O3/Mn0.5Zn0.5Fe2O4 thermosensitive magnetoliposome contained P, Mn, Zn, Fe, and As elements, and this proved liposomes have successfully entrapped As2O3 and Mn0.5Zn0.5Fe2O4. The encapsulation ratio of As2O3 detected by atom fluorescence spectrophotometer was 82.16%. The result of heating test showed that Mn0.5Zn0.5Fe2O4 can serve as a heating source upon alternating magnetic field (AMF) exposure leading the nanosized thermosensitive liposomes to reach its phase transition temperature (42.52 degrees C) and release As2O3. MTT assay and flow cytometry assay revealed that the therapeutic effect of the nanosized As2O3/Mn0.5Zn0.5Fe2O4 thermosensitive magnetoliposomes combined with hyperthermia upon AMF on MDA_MB_231 cells was much better than other groups.
authors.affiliation: School of Medicine, Southeast University, N087 Ding jia qiao, Nanjing 210009, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Ziyu
authors.initials: Z
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Jia
authors.initials: J
authors.lastname: Zhang
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Zhang
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000494:pharmacology*; D045744:Cell Line, Tumor; D005290:Ferric Compounds / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D008081:Liposomes; D008280:Magnetics; D008345:Manganese / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D010087:Oxides / Q000494:pharmacology*; D015032:Zinc / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D001152:Arsenicals; D005290:Ferric Compounds; D008081:Liposomes; D010087:Oxides; C000499:ferric oxide; D008345:Manganese; D015032:Zinc; D000077237:Arsenic Trioxide