title: Synaptotagmin 1 is necessary for the Ca2+ dependence of clathrin-mediated endocytosis.
abstract: The role of Ca²⁺ in synaptic vesicle endocytosis remains uncertain due to the diversity in various preparations where several forms of endocytosis may contribute variably in different conditions. Although recent studies have demonstrated that Ca²⁺ is important for clathrin-mediated endocytosis (CME), the mechanistic role of Ca²⁺ in CME remains to be elucidated. By monitoring CME of single vesicles in mouse chromaffin cells with cell-attached capacitance measurements that offer millisecond time resolution, we demonstrate that the dynamics of vesicle fission during CME is Ca²⁺ dependent but becomes Ca²⁺ independent in synaptotagmin 1 (Syt1) knock-out cells. Our results thus suggest that Syt1 is necessary for the Ca²⁺ dependence of CME.
authors.affiliation: Department of Biological Sciences, University of Illinois at Chicago, Chicago, Illinois 60607, USA.
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Yao
authors.forename: Yan
authors.initials: Y
authors.lastname: Rao
authors.forename: Kelly
authors.initials: K
authors.lastname: Varga
authors.forename: Chun-Yang
authors.initials: CY
authors.lastname: Wang
authors.forename: Peng
authors.initials: P
authors.lastname: Xiao
authors.forename: Manfred
authors.initials: M
authors.lastname: Lindau
authors.forename: Liang-Wei
authors.initials: LW
authors.lastname: Gong
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D019439:Chromaffin Cells / Q000378:metabolism; D002966:Clathrin / Q000502:physiology*; D004705:Endocytosis / Q000502:physiology*; D005260:Female; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D050863:Synaptotagmin I / Q000172:deficiency*
chemical_list: D002966:Clathrin; D050863:Synaptotagmin I; C494311:Syt1 protein, mouse; D002118:Calcium