title: Serum profile of pro- and anti-inflammatory cytokines in rats following implantation of low-temperature plasma-modified titanium plates.
abstract: Surface modification of Titanium (Ti) by low-temperature plasma influences cell-material interactions. Therefore, this study aimed at examining serum cytokine levels and associations after intramuscular implantation (n = 8 rats/group) of Ti-plates with Plasma Polymerized Allyl Amine (Ti-PPAAm), Plasma Polymerized Acrylic Acid (Ti-PPAAc), and without such layers (Ti-Controls). Pro-inflammatory (IL-2, IFNγ, IL-6) and anti-inflammatory (IL-4, IL-10, IL-13) cytokines were measured weekly for 56 days. Ti-PPAAm caused increased IL-2 (d7-14, d35), increased IFNγ (d35) and decreased IL-10 (d35, d49-56). Ti-PPAAc induced divergent anti-inflammatory cytokine changes with increased IL-4 (d28-56) and decreased IL-10 (d42-56). Ti-Controls elicited increased IL-2 (d42) and IFNγ (d35-42, d56). IL-6 was not detected and IL-13 only in three samples, thus they do not influence the response against these Ti implants. Correlation analysis revealed surface-dependent associations between cytokines indicating the involvement of different inflammatory cell populations. Concluding, different plasma modifications induce specific serum cytokine profiles and associations indicating distinct inflammatory responses.
authors.affiliation: Department of Medical Biochemistry and Molecular Biology, Research Group of Predictive Diagnostics, Ernst Moritz Arndt University of Greifswald, Greifswalder Str. 11c, 17495 Karlsburg, Germany.
authors.forename: Uwe
authors.initials: U
authors.lastname: Walschus
authors.forename: Andreas
authors.initials: A
authors.lastname: Hoene
authors.forename: Maciej
authors.initials: M
authors.lastname: Patrzyk
authors.forename: Birgit
authors.initials: B
authors.lastname: Finke
authors.forename: Martin
authors.initials: M
authors.lastname: Polak
authors.forename: Silke
authors.initials: S
authors.lastname: Lucke
authors.forename: Barbara
authors.initials: B
authors.lastname: Nebe
authors.forename: Karsten
authors.initials: K
authors.lastname: Schroeder
authors.forename: Andreas
authors.initials: A
authors.lastname: Podbielski
authors.forename: Lutz
authors.initials: L
authors.lastname: Wilhelm
authors.forename: Michael
authors.initials: M
authors.lastname: Schlosser
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000097:blood* / Q000378:metabolism; D020099:Coated Materials, Biocompatible / Q000494:pharmacology; D055117:Curing Lights, Dental; D016207:Cytokines / Q000097:blood* / Q000378:metabolism; D004864:Equipment and Supplies / Q000009:adverse effects; D018836:Inflammation Mediators / Q000097:blood* / Q000378:metabolism; D008297:Male; D055442:Metabolome / Q000187:drug effects; D058626:Plasma Gases / Q000737:chemistry / Q000494:pharmacology; D019919:Prosthesis Implantation / Q000009:adverse effects / Q000534:rehabilitation; D051381:Rats; D011917:Rats, Inbred Lew; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000893:Anti-Inflammatory Agents; D020099:Coated Materials, Biocompatible; D016207:Cytokines; D018836:Inflammation Mediators; D058626:Plasma Gases; D014025:Titanium