title: Phytoremediation of trichlorophenol by Phase II metabolism in transgenic Arabidopsis overexpressing a Populus glucosyltransferase.
abstract: Trichlorophenol (TCP) and its derivatives are introduced into the environment through numerous sources, including wood preservatives and biocides. Environmental contamination by TCPs is associated with human health risks, necessitating the development of cost-effective remediation techniques. Efficient phytoremediation of TCP is potentially feasible because it contains a hydroxyl group and is suitable for direct phase II metabolism. In this study, we present a system for TCP phytoremediation based on sugar conjugation by overexpressing a Populus putative UDP-glc-dependent glycosyltransferase (UGT). The enzyme PtUGT72B1 displayed the highest TCP-conjugating activity among all reported UGTs. Transgenic Arabidopsis demonstrated significantly enhanced tolerances to 2,4,5-TCP and 2,4,6-TCP. Transgenic plants also exhibited a strikingly higher capacity to remove TCP from their media. This work indicates that Populus UGT overexpression in Arabidopsis may be an efficient method for phytoremoval and degradation of TCP. Our findings have the potential to provide a suitable remediation strategy for sites contaminated by TCP.
authors.affiliation: College of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Zhen-Hong
authors.initials: ZH
authors.lastname: Su
authors.forename: Zhi-Sheng
authors.initials: ZS
authors.lastname: Xu
authors.forename: Ri-He
authors.initials: RH
authors.lastname: Peng
authors.forename: Yong-Sheng
authors.initials: YS
authors.lastname: Tian
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.forename: Hong-Juan
authors.initials: HJ
authors.lastname: Han
authors.forename: Quan-Hong
authors.initials: QH
authors.lastname: Yao
authors.forename: Ai-Zhong
authors.initials: AZ
authors.lastname: Wu
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D001673:Biodegradation, Environmental / Q000187:drug effects; D002733:Chlorophenols / Q000302:isolation & purification / Q000378:metabolism* / Q000633:toxicity; D002851:Chromatography, High Pressure Liquid; D005798:Genes, Bacterial / Q000235:genetics; D005964:Glucosyltransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006031:Glycosylation / Q000187:drug effects; D006801:Humans; D007700:Kinetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010843:Pichia / Q000378:metabolism; D030821:Plants, Genetically Modified; D032107:Populus / Q000201:enzymology* / Q000235:genetics; D013379:Substrate Specificity / Q000187:drug effects
chemical_list: D002733:Chlorophenols; C009534:2,4,5-trichlorophenol; D005964:Glucosyltransferases; C024564:2,4,6-trichlorophenol