title: The Arabidopsis AP2/ERF transcription factor RAP2.11 modulates plant response to low-potassium conditions.
abstract: Plants respond to low-nutrient conditions through metabolic and morphology changes that increase their ability to survive and grow. The transcription factor RAP2.11 was identified as a component in the response to low potassium through regulation of the high-affinity K(+) uptake transporter AtHAK5 and other components of the low-potassium signal transduction pathway. RAP2.11 was identified through the activation tagging of Arabidopsis lines that contained a luciferase marker driven by the AtHAK5 promoter that is normally only induced by low potassium. This factor bound to a GCC-box of the AtHAK5 promoter in vitro and in vivo. Transcript profiling revealed that a large number of genes were up-regulated in roots by RAP2.11 overexpression. Many regulated genes were identified to be in functional categories that are important in low-K(+) signaling. These categories included ethylene signaling, reactive oxygen species production, and calcium signaling. Promoter regions of the up-regulated genes were enriched in the GCCGGC motif also contained in the AtHAK5 promoter. These results suggest that RAP2.11 regulates AtHAK5 expression under low-K(+) conditions and also contributes to a coordinated response to low-potassium conditions through the regulation of other genes in the low-K(+) signaling cascade.
authors.affiliation: Donald Danforth Plant Science Center, St Louis, MO 63132, USA.
authors.forename: Min Jung
authors.initials: MJ
authors.lastname: Kim
authors.forename: Daniel
authors.initials: D
authors.lastname: Ruzicka
authors.forename: Ryoung
authors.initials: R
authors.lastname: Shin
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Schachtman
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001692:Biological Transport; D018506:Gene Expression Regulation, Plant; D011188:Potassium / Q000378:metabolism*; D027961:Potassium-Hydrogen Antiporters; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D027981:Symporters / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D027961:Potassium-Hydrogen Antiporters; C577740:RAP2.11 protein, Arabidopsis; D027981:Symporters; D014157:Transcription Factors; C501258:potassium transporter, Arabidopsis; D011188:Potassium