title: TNPO3 is required for HIV-1 replication after nuclear import but prior to integration and binds the HIV-1 core.
abstract: TNPO3 is a nuclear importer required for HIV-1 infection. Here, we show that depletion of TNPO3 leads to an HIV-1 block after nuclear import but prior to integration. To investigate the mechanistic requirement of TNPO3 in HIV-1 infection, we tested the binding of TNPO3 to the HIV-1 core and found that TNPO3 binds to the HIV-1 core. Overall, this work suggests that TNPO3 interacts with the incoming HIV-1 core in the cytoplasm to assist a process that is important for HIV-1 infection after nuclear import.
authors.affiliation: Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York, USA.
authors.forename: Jose Carlos
authors.initials: JC
authors.lastname: Valle-Casuso
authors.forename: Francesca
authors.initials: F
authors.lastname: Di Nunzio
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.forename: Natalia
authors.initials: N
authors.lastname: Reszka
authors.forename: Maritza
authors.initials: M
authors.lastname: Lienlaf
authors.forename: Nathalie
authors.initials: N
authors.lastname: Arhel
authors.forename: Patricio
authors.initials: P
authors.lastname: Perez
authors.forename: Abraham L
authors.initials: AL
authors.lastname: Brass
authors.forename: Felipe
authors.initials: F
authors.lastname: Diaz-Griffero
mesh_terms: D021581:Active Transport, Cell Nucleus; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D003593:Cytoplasm / Q000235:genetics / Q000378:metabolism / Q000821:virology; D015658:HIV Infections / Q000235:genetics / Q000378:metabolism* / Q000821:virology*; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D006801:Humans; D011485:Protein Binding; D016662:Virus Integration; D014779:Virus Replication; D028961:beta Karyopherins / Q000235:genetics / Q000378:metabolism*; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000235:genetics / Q000378:metabolism*
chemical_list: C546476:TNPO3 protein, human; D028961:beta Karyopherins; D054301:gag Gene Products, Human Immunodeficiency Virus