title: Generation of functional coatings on hydrophobic surfaces through deposition of denatured proteins followed by grafting from polymerization.
abstract: Hydrophilic coatings were produced on flat hydrophobic substrates featuring n-octadecyltrichlorosilane (ODTS) and synthetic polypropylene (PP) nonwoven surfaces through the adsorption of denatured proteins. Specifically, physisorption from aqueous solutions of α-lactalbumin, lysozyme, fibrinogen, and two soy globulin proteins (glycinin and β-conglycinin) after chemical (urea) and thermal denaturation endowed the hydrophobic surfaces with amino and hydroxyl functionalities, yielding enhanced wettability. Proteins adsorbed strongly onto ODTS and PP through nonspecific interactions. The thickness of adsorbed heat-denatured proteins was adjusted by varying the pH, protein concentration in solution, and adsorption time. In addition, the stability of the immobilized protein layer was improved significantly after interfacial cross-linking with glutaraldehyde in the presence of sodium borohydride. The amino and hydroxyl groups present on the protein-modified surfaces served as reactive sites for the attachment of polymerization initiators from which polymer brushes were grown by surface-initiated atom-transfer radical polymerization of 2-hydroxyethyl methacrylate. Protein denaturation and adsorption as well as the grafting of polymeric brushes were characterized by circular dichroism, ellipsometry, contact angle, and Fourier transform infrared spectroscopy in the attenuated total reflection mode.
authors.affiliation: Department of Materials Science and Engineering, North Carolina State University, Raleigh, North Carolina 27695-7907, USA.
authors.forename: Kiran K
authors.initials: KK
authors.lastname: Goli
authors.forename: Orlando J
authors.initials: OJ
authors.lastname: Rojas
authors.forename: A Evren
authors.initials: AE
authors.lastname: Ozçam
authors.forename: Jan
authors.initials: J
authors.lastname: Genzer
mesh_terms: D000327:Adsorption; D052179:Antigens, Plant / Q000737:chemistry*; D005916:Globulins / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D058105:Polymerization; D011126:Polypropylenes / Q000737:chemistry; D011489:Protein Denaturation / Q000187:drug effects; D055550:Protein Stability; D055314:Seed Storage Proteins / Q000737:chemistry*; D012821:Silanes / Q000737:chemistry; D030262:Soybean Proteins / Q000737:chemistry*; D013499:Surface Properties; D013696:Temperature; D014508:Urea / Q000494:pharmacology
chemical_list: D052179:Antigens, Plant; D005916:Globulins; D011126:Polypropylenes; D055314:Seed Storage Proteins; D012821:Silanes; D030262:Soybean Proteins; C013894:beta-conglycinin protein, Glycine max; C061189:octadecyltrichlorosilane; D014508:Urea; C011474:glycinin