title: A Rab11A/myosin Vb/Rab11-FIP2 complex frames two late recycling steps of langerin from the ERC to the plasma membrane.
abstract: A large body of knowledge relating to the constitution of Rab GTPase/Rab effector complexes and their impact on both membrane domain organization and overall membrane trafficking has been built up in recent years. However in the context of the live cell there are still many questions that remain to be answered, such as where and when these complexes assemble and where they perform their primary function(s). We describe here the dynamic processes that take place in the final steps of the Rab11A dependent recycling pathway, in the context of the membrane platform constituted by Myosin Vb, Rab11A, and Rab11-FIP2. We first confirm that a series of previously reported observations obtained during the study of a number of trafficking cargoes also apply to langerin. Langerin is a cargo molecule that traffics through Rab11A-positive membrane domains of the endosomal recycling pathway. In order to explore the relative dynamics of this set of partners, we make extensive use of a combinatory approach of Live-FRET, fast FRAP video, fast confocal and TIRF microscopy modalities. Our data show that the Myosin Vb/Rab11A/Rab11-FIP2 platform is spatially involved in the regulation of langerin trafficking at two distinct sites within live cells, first at the sorting site in the endosomal recycling compartment (ERC) where transport vesicles are formed, and subsequently, in a strict time-defined order, at the very late stage of docking/tethering and fusion of these langerin recycling vesicles to the plasma membrane.
authors.affiliation: UMR 144, Molecular Mechanisms of Intracellular Transport Laboratory, CNRS-Institut Curie, 26 Rue d'Ulm, 75248 Paris Cedex 05, France.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Gidon
authors.forename: Sabine
authors.initials: S
authors.lastname: Bardin
authors.forename: Bertrand
authors.initials: B
authors.lastname: Cinquin
authors.forename: Jerome
authors.initials: J
authors.lastname: Boulanger
authors.forename: François
authors.initials: F
authors.lastname: Waharte
authors.forename: Laurent
authors.initials: L
authors.lastname: Heliot
authors.forename: Henri
authors.initials: H
authors.lastname: de la Salle
authors.forename: Daniel
authors.initials: D
authors.lastname: Hanau
authors.forename: Charles
authors.initials: C
authors.lastname: Kervrann
authors.forename: Bruno
authors.initials: B
authors.lastname: Goud
authors.forename: Jean
authors.initials: J
authors.lastname: Salamero
mesh_terms: D015703:Antigens, CD / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D011992:Endosomes / Q000378:metabolism*; D031541:Fluorescence Resonance Energy Transfer; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D037181:Lectins, C-Type / Q000378:metabolism*; D037241:Mannose-Binding Lectins / Q000378:metabolism*; D008545:Melanoma / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D018613:Microscopy, Confocal / Q000379:methods; D018995:Myosin Heavy Chains / Q000378:metabolism*; D024701:Myosin Type V / Q000378:metabolism*; D021381:Protein Transport; D013997:Time Factors; D020691:rab GTP-Binding Proteins / Q000378:metabolism*; D000099193:rab11 GTP-Binding Proteins
chemical_list: D015703:Antigens, CD; D002352:Carrier Proteins; D037181:Lectins, C-Type; D037241:Mannose-Binding Lectins; D008565:Membrane Proteins; D018995:Myosin Heavy Chains; D024701:Myosin Type V; D020691:rab GTP-Binding Proteins; D000099193:rab11 GTP-Binding Proteins; C121916:CD207 protein, human; C505832:MYO5B protein, human; C463963:RAB11FIP2 protein, human