title: Immortalized sebocytes can spontaneously differentiate into a sebaceous-like phenotype when cultured as a 3D epithelium.
abstract: Sebocytes originate from the same lineage as keratinocytes, and both cell types may have similarities in terms of growth and differentiation. We were interested in studying the behaviour of human sebocytes when cultured in conditions validated for epidermal reconstruction. For this purpose, we established a HPV16-E6/7-immortalized human sebocyte cell line (SEBO662) growing in keratinocyte defined media. Postconfluent SEBO662 cells in monolayers express the early sebocyte marker, cytokeratin 7 (K7), do not express Epithelia Membrane Antigen (EMA) and do not exhibit strong lipogenic activity. However, when placed at the air-liquid interface, SEBO662 multilayers spontaneously differentiate into a sebaceous-like structure as shown by the strong polarized expression of the late sebaceous marker EMA, the overexpression of some lipogenic markers and lipid production on the upper side of the epithelium. This work highlights the value of simple 3D models for exhibiting spontaneous differentiation and polarization.
authors.forename: Christine
authors.initials: C
authors.lastname: Barrault
authors.forename: Isabelle
authors.initials: I
authors.lastname: Dichamp
authors.forename: Julien
authors.initials: J
authors.lastname: Garnier
authors.forename: Nathalie
authors.initials: N
authors.lastname: Pedretti
authors.forename: Franck
authors.initials: F
authors.lastname: Juchaux
authors.forename: Alain
authors.initials: A
authors.lastname: Deguercy
authors.forename: Gérard
authors.initials: G
authors.lastname: Agius
authors.forename: François-Xavier
authors.initials: FX
authors.lastname: Bernard
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D002460:Cell Line; D016895:Culture Media, Serum-Free; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism; D006801:Humans; D053552:Keratin-7 / Q000378:metabolism; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism; D050356:Lipid Metabolism; D010641:Phenotype; D012627:Sebaceous Glands / Q000166:cytology* / Q000378:metabolism
chemical_list: D015415:Biomarkers; D016895:Culture Media, Serum-Free; D053552:Keratin-7