title: Expression of cholecystokinin-1 receptor is correlated with proteinuria in human diabetic nephropathy.
abstract: This study aimed to examine the expression of cholecystokinin-1 receptor (CCK-1R) in the kidneys of type 2 diabetic nephropathy (DN) and the correlation of CCK-1R mRNA and proteinuria. Localization of CCK-1R in kidney of diabetic patient with nephropathy was examined by immunohistochemistry and in situ hybridization. The glomeruli did not express CCK-1R in either control or diabetic nephropathic kidneys. However, the expressions of CCK-1R protein and mRNA in tubules were significantly increased in DN, which had no relationship with the severity of DN. Furthermore, there was a positive correlation between the percentage of cells positive for CCK-1R mRNA and the degree of proteinuria. Increased CCK-1R expression could be demonstrated in the tubules and the CCK-1R might be implicated in the development of proteinuria in human DN.
authors.affiliation: Department of Nephrology, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
authors.forename: Mingao
authors.initials: M
authors.lastname: Wang
authors.forename: Rujuan
authors.initials: R
authors.lastname: Xie
authors.forename: Ruichan
authors.initials: R
authors.lastname: Liu
authors.forename: Xibei
authors.initials: X
authors.lastname: Jia
authors.forename: Yushi
authors.initials: Y
authors.lastname: Bao
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Liu
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D001706:Biopsy; D003924:Diabetes Mellitus, Type 2 / Q000150:complications*; D003928:Diabetic Nephropathies / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology* / Q000652:urine; D018450:Disease Progression; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007678:Kidney Glomerulus / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D007684:Kidney Tubules / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D008875:Middle Aged; D011507:Proteinuria / Q000209:etiology* / Q000503:physiopathology; D012333:RNA, Messenger / Q000378:metabolism; D043886:Receptor, Cholecystokinin A / Q000378:metabolism*; D012720:Severity of Illness Index; D015854:Up-Regulation
chemical_list: D015415:Biomarkers; D012333:RNA, Messenger; D043886:Receptor, Cholecystokinin A