title: Plasma concentrations but not serum concentrations of brain-derived neurotrophic factor are related to pro-inflammatory cytokines in patients undergoing major abdominal surgery.
abstract: OBJECTIVE
  To investigate peripheral brain-derived neurotrophic factor (BDNF) concentrations in the perioperative period, their relationship with transforming growth factor-β1 (TGF-β1 tumour necrosis factor-α (TNF-α), interleukin-6 (IL-6) and IL-6 genetics.
  
  
  METHODS
  Prospective, observational study. BDNF, TGF-β1, IL-6 and TNF-α were analysed at baseline (T0), 5 h (T1), 24 h (T2) and 5 days (T3) after surgery, in 21 patients. The IL-6 -174 G/C polymorphism was genotyped.
  
  
  RESULTS
  Serum BDNF concentrations decreased (P=0.048), correlated with TGF-β1 (r=0.610 at T1, r=0.493 at T2, r=0.554 at T3). Plasma BDNF concentrations raised (P=0.049), correlated with IL-6 and TNF-α at T1 (r=0.495 and r=0.441, respectively). BDNF response was predictable from TNF-α and IL-6 concentrations and the IL-6 -174 G/C genotype.
  
  
  CONCLUSIONS
  Serum and plasma BDNF concentrations could relate to platelet activation and inflammatory response, respectively. IL-6 genetics played a role in the BDNF acute response.
authors.affiliation: Department of Biosciences, Biotechnologies and Pharmacological Sciences, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Chimienti
authors.forename: A
authors.initials: A
authors.lastname: Mezzapesa
authors.forename: M T
authors.initials: MT
authors.lastname: Rotelli
authors.forename: L
authors.initials: L
authors.lastname: Lupo
authors.forename: G
authors.initials: G
authors.lastname: Pepe
mesh_terms: D000005:Abdomen; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001792:Blood Platelets / Q000276:immunology; D019208:Brain-Derived Neurotrophic Factor / Q000097:blood*; D016207:Cytokines / Q000097:blood* / Q000276:immunology; D013505:Digestive System Surgical Procedures; D005260:Female; D006801:Humans; D007249:Inflammation / Q000097:blood* / Q000276:immunology; D015850:Interleukin-6 / Q000097:blood / Q000235:genetics / Q000276:immunology; D008297:Male; D008875:Middle Aged; D059035:Perioperative Period; D010949:Plasma; D015539:Platelet Activation; D020641:Polymorphism, Single Nucleotide; D044967:Serum; D053773:Transforming Growth Factor beta1 / Q000097:blood / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000276:immunology
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D016207:Cytokines; D015850:Interleukin-6; D053773:Transforming Growth Factor beta1; D014409:Tumor Necrosis Factor-alpha