title: Dexamethasone inhibits TGF-β2-induced migration of human lens epithelial cells: implications for posterior capsule opacification prevention.
abstract: The elevation of transforming growth factor-β2 (TGF-β2) levels in eye tissue is considered as one of the major factors contributing to posterior capsule opacification (PCO) in patients undergoing cataract surgery, since TGF-β2 is known to stimulate the cell migration of residual human lens epithelial cells (HLECs). The present study aimed to test the potential effect of dexamethasone (DEX) on TGF-β2-induced cell migration and the possible cellular mechanisms involved in this process. Cultured HLE-B3 cells were treated with TGF-β2 (0.1 ng/ml) in the presence or absence of DEX (100 nM). HLE-B3 cell migration was determined by the Phagokinetic Track Motility Assay. Activation of mitogen-activated protein kinase (MAPK) signaling pathways was determined by Western blotting using specific phosphorylation antibodies, matrix metalloproteinase (MMP)-2 and MMP-9 mRNA expression, and activities were analyzed by RT-PCR and gelatin zymography assay, respectively. In cultured HLE-B3 cells, DEX largely inhibited TGF-β2-induced cell migration and MMP activity, probably by inhibiting the ERK/MAPK pathway. We suggest that the use of DEX may be of help in the prevention of PCO formation and development.
authors.affiliation: The Affiliated Eye Hospital of Nanjing Medical University, Nanjing, People's Republic of China.
authors.forename: Jin
authors.initials: J
authors.lastname: Yao
authors.forename: Wen
authors.initials: W
authors.lastname: Yang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.forename: Yan-Xi
authors.initials: YX
authors.lastname: Sun
authors.forename: Qin
authors.initials: Q
authors.lastname: Jiang
mesh_terms: D058442:Capsule Opacification / Q000517:prevention & control*; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D003907:Dexamethasone / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007908:Lens, Crystalline / Q000166:cytology* / Q000187:drug effects; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D053781:Transforming Growth Factor beta2 / Q000494:pharmacology*; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D004791:Enzyme Inhibitors; D053781:Transforming Growth Factor beta2; D003907:Dexamethasone; D020928:Mitogen-Activated Protein Kinases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9