title: Alpha-1-antitrypsin acts as a preeclampsia-related protein: a proteomic study.
abstract: OBJECTIVE
  To screen the preeclampsia-related protein by proteomics.
  
  
  METHODS
  Proteomics was performed to identify differential protein expression profiles between normal full-term pregnancy, early-onset severe preeclampsia (ES-PE) or late-onset severe preeclampsia (LS-PE; n = 10 per group). Real-time quantitative PCR and immunohistochemistry were conducted to confirm the expression of α(1)-antitrypsin (α(1)-AT) in the decidual tissues of different subjects. ELISA was employed to detect the α(1)-AT content in the peripheral blood of 90 women (n = 30 per group).
  
  
  RESULTS
  We successfully constructed two-dimensional electrophoresis maps of decidual tissues, and a total of 20 differentially expressed proteins were identified. The α(1)-AT expression was different among the three groups. The normal full-term pregnancy women expressed the most α(1)-AT, and the LS-PE women expressed the least amount of α(1)-AT. The difference in the α(1)-AT expression was consistent with the proteomics data. The peripheral α(1)-AT content was the highest in the normal full-term pregnancy group (1.85 ± 0.15 g/l), moderate in the ES-PE group (0.77 ± 0.14 g/l) and lowest in the LS-PE group (0.42 ± 0.07 g/l; p < 0.05).
  
  
  CONCLUSIONS
  Using 2D PAGE, we identified twenty proteins with significantly altered expression in PE. These differentially expressed proteins include prevention protein, in which α(1)-AT is downregulated in PE.
authors.affiliation: Department of Obstetrics and Gynecology, Shenzhen Baoan People's Hospital, Shenzhen, China.
authors.forename: Ya-Ling
authors.initials: YL
authors.lastname: Feng
authors.forename: Chang-Ju
authors.initials: CJ
authors.lastname: Zhou
authors.forename: Xiao-Mao
authors.initials: XM
authors.lastname: Li
authors.forename: Xiao-Qin
authors.initials: XQ
authors.lastname: Liang
mesh_terms: D016022:Case-Control Studies; D017931:DNA Primers / Q000737:chemistry; D003656:Decidua / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005865:Gestational Age; D006801:Humans; D007150:Immunohistochemistry; D011225:Pre-Eclampsia / Q000097:blood* / Q000235:genetics; D011247:Pregnancy; D040901:Proteomics / Q000379:methods*; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D015842:Serine Proteinase Inhibitors / Q000097:blood* / Q000235:genetics; D000515:alpha 1-Antitrypsin / Q000097:blood* / Q000235:genetics
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D015842:Serine Proteinase Inhibitors; D000515:alpha 1-Antitrypsin