title: HPLC quantitative analysis of insecticidal didehydrostemofoline and stemofoline in Stemona collinsiae root extracts.
abstract: BACKGROUND
  Stemona spp. have been traditionally used as natural pesticides and medicinal plants. Stemona collinsiae Craib has been of interest for its insecticidal activity, which has been supported by many scientific research studies. The roots contain didehydrostemofoline and stemofoline alkaloids as active components.
  
  
  OBJECTIVE
  To develop and validate a HPLC method for the quantitative analysis of didehydrostemofoline and stemofoline in S. collinsiae root extracts.
  
  
  METHODS
  HPLC was carried out using a Hypersil BDS C₁₈-column eluted with methanol:1 m m ammonium acetate (55:45) with a flow rate of 1 mL/min and detection at 295 nm. Method validation was performed to assure its linearity, precision, accuracy and limits of detection and quantitation.
  
  
  RESULTS
  Didehydrostemofoline and stemofoline showed a linear relationship within the range of 0.5-432.4 and 0.5-188.4 µg/mL, respectively. The method was shown to be precise with RSD < 2%. The average recovery of didehydrostemofoline and stemofoline were 98.80 and 99.97%, respectively. Eight samples of S. collinsiae root extracts were analysed and the average contents of didehydrostemofoline and stemofoline were 0.78 and 0.048% w/w, respectively.
  
  
  CONCLUSIONS
  The HPLC method developed was appropriate for the analysis of didehydrostemofoline and stemofoline in S. collinsiae root extracts. This work would be useful as a guide for the standardisation of S. collinsiae root extract raw materials and their products as natural pesticides.
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand.
authors.forename: Sumet
authors.initials: S
authors.lastname: Kongkiatpaiboon
authors.forename: Wandee
authors.initials: W
authors.lastname: Gritsanapan
mesh_terms: D000470:Alkaloids / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006576:Heterocyclic Compounds, 4 or More Rings / Q000032:analysis* / Q000737:chemistry; D016014:Linear Models; D010936:Plant Extracts / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry*; D012680:Sensitivity and Specificity; D041901:Stemonaceae / Q000737:chemistry*
chemical_list: D000470:Alkaloids; D006576:Heterocyclic Compounds, 4 or More Rings; D010936:Plant Extracts; C481859:didehydrostemofoline; C430220:stemofoline