title: Biodegradability and biocompatibility study of poly(chitosan-g-lactic acid) scaffolds.
abstract: A biodegradable, biocompatible poly(chitosan-g-lactic acid) (PCLA) scaffold was prepared and evaluated in vitro and in vivo. The PCLA scaffold was obtained by grafting lactic acid (LA) onto the amino groups on chitosan (CS) without a catalyst. The PCLA scaffolds were characterized by Fourier Transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM). The biodegradabilty was determined by mass loss in vitro, and degradation in vivo as a function of feed ratio of LA/CS. Bone marrow mesenchymal stem cell (BMSC) culture experiments and histological examination were performed to evaluate the PCLA scaffolds' biocompatibility. The results indicated that PCLA was promising for tissue engineering application.
authors.affiliation: Institute of Biochemical and Biotechnological Drugs, School of Pharmacy, Shandong University, Jinan 250012, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Zhang
authors.forename: Huifei
authors.initials: H
authors.lastname: Cui
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D001854:Bone Marrow Cells / Q000502:physiology; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D048271:Chitosan / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000502:physiology; D051379:Mice; D009113:Muramidase / Q000737:chemistry; D011091:Polyesters / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D016062:Porosity; D011897:Random Allocation; D051381:Rats; D017550:Spectroscopy, Fourier Transform Infrared; D040521:Subcutaneous Tissue / Q000473:pathology; D013499:Surface Properties; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; C531321:poly(chitosan-g-lactic acid); D048271:Chitosan; D009113:Muramidase