title: Detection of human papilloma viruses using nanostructurated silicon support in microarray technology.
abstract: In a typical microarray experiment, DNA is arrayed on a solid substrate as spots, the array being probed with a sample or a capture molecule of interest and the interaction monitored through different detection methods. The present study evaluates the possibility to use micro-array technology to genotype samples with Human Papilloma Viruses (HPV). The performance of DNA microarrays strongly depend on their surface properties. The efficiency of DNA immobilization in terms of sensitivity and specificity is one of the most important step in obtaining a microarray chip for diagnosis of HPV family viruses. Here we report the preparation and evaluation of nano-porous silicon surfaces for HPV detection based on DNA micro-array technique. Two different surfaces based on similar porous structure chemically modified in order to efficiently immobilize ss-DNA specific for HPV viruses were investigate.
authors.affiliation: Laboratory of Nanotechnology, National Institute for Research and Development in Microtechnologies (IMT-Bucharest) 077190, Romania.
authors.forename: Monica
authors.initials: M
authors.lastname: Simion
authors.forename: Lorand
authors.initials: L
authors.lastname: Savu
authors.forename: Antonio
authors.initials: A
authors.lastname: Radoi
authors.forename: Mihaela
authors.initials: M
authors.lastname: Miu
authors.forename: Adina
authors.initials: A
authors.lastname: Bragaru
mesh_terms: D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics; D005838:Genotype; D006801:Humans; D007103:Immobilization / Q000379:methods; D046228:Microarray Analysis / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry*; D009693:Nucleic Acid Hybridization / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D012825:Silicon / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D004279:DNA, Viral; D012825:Silicon