title: Stochastic sensing of proteins with receptor-modified solid-state nanopores.
abstract: Solid-state nanopores are capable of the label-free analysis of single molecules. It is possible to add biochemical selectivity by anchoring a molecular receptor inside the nanopore, but it is difficult to maintain single-molecule sensitivity in these modified nanopores. Here, we show that metallized silicon nitride nanopores chemically modified with nitrilotriacetic acid receptors can be used for the stochastic sensing of proteins. The reversible binding and unbinding of the proteins to the receptors is observed in real time, and the interaction parameters are statistically analysed from single-molecule binding events. To demonstrate the versatile nature of this approach, we detect His-tagged proteins and discriminate between the subclasses of rodent IgG antibodies.
authors.affiliation: Walter Schottky Institute & Institute for Advanced Study, Technische Universität München, Am Coulombwall 4, 85748                 Garching, Germany.
authors.forename: Ruoshan
authors.initials: R
authors.lastname: Wei
authors.forename: Volker
authors.initials: V
authors.lastname: Gatterdam
authors.forename: Ralph
authors.initials: R
authors.lastname: Wieneke
authors.forename: Robert
authors.initials: R
authors.lastname: Tampé
authors.forename: Ulrich
authors.initials: U
authors.lastname: Rant
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D003627:Data Interpretation, Statistical; D004867:Equipment Design; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D036103:Nanotechnology / Q000295:instrumentation*; D016062:Porosity; D025941:Protein Interaction Mapping / Q000295:instrumentation*; D011506:Proteins / Q000032:analysis*; D013269:Stochastic Processes
chemical_list: D011506:Proteins