title: New monodisperse magnetic polymer microspheres biofunctionalized for enzyme catalysis and bioaffinity separations.
abstract: Magnetic macroporous PGMA and PHEMA microspheres containing carboxyl groups are synthesized by multi-step swelling and polymerization followed by precipitation of iron oxide inside the pores. The microspheres are characterized by SEM, IR spectroscopy, AAS, and zeta-potential measurements. Their functional groups enable bioactive ligands of various sizes and chemical structures to couple covalently. The applicability of these monodisperse magnetic microspheres in biospecific catalysis and bioaffinity separation is confirmed by coupling with the enzyme trypsin and huIgG. Trypsin-modified magnetic PGMA-COOH and PHEMA-COOH microspheres are investigated in terms of their enzyme activity, operational and storage stability. The presence of IgG molecules on microspheres is confirmed.
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic. horak@imc.cas.cz
authors.forename: Daniel
authors.initials: D
authors.lastname: Horák
authors.forename: Jana
authors.initials: J
authors.lastname: Kučerová
authors.forename: Lucie
authors.initials: L
authors.lastname: Korecká
authors.forename: Barbora
authors.initials: B
authors.lastname: Jankovičová
authors.forename: Jiří
authors.initials: J
authors.lastname: Palarčík
authors.forename: Petr
authors.initials: P
authors.lastname: Mikulášek
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Bílková
mesh_terms: D055162:Biocatalysis; D002846:Chromatography, Affinity / Q000379:methods*; D005290:Ferric Compounds / Q000737:chemistry; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry; D007074:Immunoglobulin G / Q000737:chemistry; D059346:Magnets; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D011102:Polyhydroxyethyl Methacrylate / Q000138:chemical synthesis*; D058105:Polymerization; D011109:Polymethacrylic Acids / Q000138:chemical synthesis*; D013055:Spectrophotometry, Infrared; D014357:Trypsin / Q000737:chemistry
chemical_list: D005290:Ferric Compounds; D055767:Immobilized Proteins; D007074:Immunoglobulin G; D011109:Polymethacrylic Acids; C000499:ferric oxide; C042535:polyglycidyl methacrylate; D011102:Polyhydroxyethyl Methacrylate; D014357:Trypsin