title: A new isoflavone glycitein 7-O-beta-D-glucoside 4''-O-methylate, isolated from Cordyceps militaris grown on germinated soybeans extract, inhibits EGF-induced mucus hypersecretion in the human lung mucoepidermoid cells.
abstract: A new isoflavone glycitein 7-O-beta-d-glucoside 4''-O-methylate (CGLM) has been isolated recently from Cordyceps militaris grown on germinated soybean extract and has antioxidant activity. In the present study, CGLM was investigated for its suppression of airway mucous hyper-secretion in epidermal growth factor (EGF)-treated human lung mucoepidermoid cells. NCI-H292 cells were treated with CGLM for 1 h, followed by EGF treatment for 24 h. The decrease in cyclooxygenase-2 (COX-2) production was correlated with reduced levels of protein and mRNA of inducible matrix metalloproteinase 9 (MMP-9) and also MUC5AC gene expression. CGLM directly inhibited down-regulated NF-κB activity, and significantly inhibited the phosphorylation of p38 and ERK1/2 (p42/p44) in NCI-H292 cells. These results suggest that CGLM protects NCI-H292 cells from EGF-induced damage by down-regulation of COX-2, MMP-9 and MUC5AC gene expression, mediated via blocking the NF-kappa-B and p38/ERK MAPK pathways.
authors.affiliation: Department of Bioscience and Biotechnology, BIMC, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Jung-Hee
authors.initials: JH
authors.lastname: Kim
authors.forename: Dong Ki
authors.initials: DK
authors.lastname: Park
authors.forename: Choong Hwan
authors.initials: CH
authors.lastname: Lee
authors.forename: Do-Young
authors.initials: DY
authors.lastname: Yoon
mesh_terms: D002460:Cell Line; D002470:Cell Survival; D032661:Cordyceps / Q000737:chemistry*; D051546:Cyclooxygenase 2 / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004847:Epithelial Cells / Q000187:drug effects*; D006801:Humans; D007529:Isoflavones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008168:Lung / Q000166:cytology; D020935:MAP Kinase Signaling System; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D055271:Mucin 5AC / Q000378:metabolism; D009093:Mucus / Q000378:metabolism*; D010766:Phosphorylation; D013025:Glycine max; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D007529:Isoflavones; C524678:MUC5AC protein, human; D055271:Mucin 5AC; D004815:Epidermal Growth Factor; C086566:glycitein; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D048051:p38 Mitogen-Activated Protein Kinases; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9