title: Biosensing of reactive intermediates produced by the photocatalytic activities of titanium dioxide nanoparticles.
abstract: The development of an enzyme based biosensing method is described for evaluating the toxicity of solutions treated by titanium dioxide photocatalysis. The method is based on the potential of rat liver microsomal glutathione transferase ability (mGST) to get enhanced in the conditions of chemical and oxidative toxicity. Phenol is taken as a model pollutant due to its toxicity and prevalence in industrial processes. Chemical analysis of the parent compound, products and acute toxicity assays using the mGST activity, were conducted during and after the various photocatalytic treatments. The maximum mGST activity was observed from 60 and 120 min treated samples. This post-treatment toxicity might be due to toxic phenolic products, which may include p-benzoquinone, hydroquinone, benzenetriol and other intermediates. The enzymatic activity pattern observed after photocatalytic treatment corresponded well with the chemical degradation data obtained by HPLC-UV. The mGST assay seems to be an easy to use and promising approach for evaluating the effectiveness of wastewater treatment processes.
authors.affiliation: Laboratoire de Génie Enzymatique et Cellulaire, UMR 6022 CNRS, Université de Technologie de Compiègne, BP 20529-60205 Compiègne Cedex, France. tanvirsh@utc.fr
authors.forename: Shazia
authors.initials: S
authors.lastname: Tanvir
authors.forename: Franck
authors.initials: F
authors.lastname: Merlier
authors.forename: Sylviane
authors.initials: S
authors.lastname: Pulvin
mesh_terms: D000818:Animals; D016227:Benzoquinones / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D002384:Catalysis; D005982:Glutathione Transferase / Q000378:metabolism; D006873:Hydroquinones / Q000737:chemistry; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology; D053758:Nanoparticles; D016877:Oxidants / Q000737:chemistry*; D019800:Phenol / Q000737:chemistry*; D055668:Photochemical Processes; D051381:Rats; D014025:Titanium / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000737:chemistry*; D018508:Water Purification / Q000379:methods
chemical_list: D016227:Benzoquinones; D006873:Hydroquinones; D016877:Oxidants; D014874:Water Pollutants, Chemical; C009495:titanium dioxide; D019800:Phenol; C004532:quinone; D014025:Titanium; D005982:Glutathione Transferase; C031927:hydroquinone