title: Identification of cis-regulatory elements specific for different types of reactive oxygen species in Arabidopsis thaliana.
abstract: The type of reactive oxygen species (ROS) is a major factor that determines the specificity of biological responses. These responses may be elicited by activation of transcription factors that recognize ROS-specific cis-regulatory elements in target genes. In search for Arabidopsis promoter motifs specific for particular types of ROS, genome-wide microarray expression profiles for 283 abiotic stress-related conditions were subjected to cluster analysis to identify gene groups induced by singlet oxygen, superoxide radicals, and H(2)O(2). Promoters of these gene groups were analyzed to identify cis-regulatory elements that are associated with specific types of ROS. Eleven ROS-specific de novo identified elements, seven known promoter motifs and several sequences enriched in ROS-responsive clusters but lacking in specificity are reported. The conservation of the identified motifs was determined in orthologous genes in C. papaya, V. vinifera and P. trichocarpa. Finally, biological functions were attributed to the motifs by calculation of GO-term enrichment for genes with conserved ROS-responsive elements.
authors.affiliation: Department of Plant Physiology and Molecular Biology, University of Plovdiv, 24 Tsar Assen str., Plovdiv 4000, Bulgaria. vepetrov@uni-plovdiv.bg
authors.forename: Veselin
authors.initials: V
authors.lastname: Petrov
authors.forename: Vanessa
authors.initials: V
authors.lastname: Vermeirssen
authors.forename: Inge
authors.initials: I
authors.lastname: De Clercq
authors.forename: Frank
authors.initials: F
authors.lastname: Van Breusegem
authors.forename: Ivan
authors.initials: I
authors.lastname: Minkov
authors.forename: Klaas
authors.initials: K
authors.lastname: Vandepoele
authors.forename: Tsanko S
authors.initials: TS
authors.lastname: Gechev
mesh_terms: D000465:Algorithms; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D001483:Base Sequence; D016000:Cluster Analysis; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D046228:Microarray Analysis; D008954:Models, Biological; D018384:Oxidative Stress / Q000235:genetics; D011401:Promoter Regions, Genetic; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics / Q000502:physiology*; D013379:Substrate Specificity / Q000235:genetics
chemical_list: D017382:Reactive Oxygen Species