title: Hair-inducing ability of human dermal papilla cells cultured under Wnt/β-catenin signalling activation.
abstract: It is well known that dermal papilla cells (DPCs) play crucial roles in hair follicle induction. In this study, we examined whether Wnt/β-catenin activation results in maintenance of the hair-inducing ability of human DPCs. Expression of DPC marker genes was maintained under Wnt/β-catenin signalling stimulation by GSK-3β inhibition. Furthermore, human DPCs showed constant hair induction when transplanted with murine epidermal cell fraction. Alu-positive human DPCs were essentially detected adjacent to the reconstructing epidermal structure positive for P-cadherin immunoreactivity. The transplanted human DPCs were abundant in the surrounding dermal sheath portion of the fully regenerated hair follicles. These results support the importance of Wnt/β-catenin signalling in hair follicle induction. This study may provide valuable information to establish a culture method of human DPCs for cell-based therapy.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Soma
authors.forename: Shigeyoshi
authors.initials: S
authors.lastname: Fujiwara
authors.forename: Yuji
authors.initials: Y
authors.lastname: Shirakata
authors.forename: Koji
authors.initials: K
authors.lastname: Hashimoto
authors.forename: Jiro
authors.initials: J
authors.lastname: Kishimoto
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D020405:Dermis / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D038362:Glycogen Synthase Kinase 3 / Q000037:antagonists & inhibitors; D000071679:Glycogen Synthase Kinase 3 beta; D006197:Hair / Q000254:growth & development*; D018859:Hair Follicle / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D014183:Transplantation, Heterologous; D019737:Transplants; D060449:Wnt Signaling Pathway / Q000187:drug effects / Q000502:physiology*; D051176:beta Catenin / Q000378:metabolism*
chemical_list: C495270:CTNNB1 protein, human; D051176:beta Catenin; C000605506:GSK3B protein, human; D000071679:Glycogen Synthase Kinase 3 beta; C000605507:Gsk3b protein, mouse; D038362:Glycogen Synthase Kinase 3