title: Improving the productivity of propionic acid with FBB-immobilized cells of an adapted acid-tolerant Propionibacterium acidipropionici.
abstract: Propionic acid is an important short-chain fatty acid with many applications, but its large-scale bioproduction was hindered by the low productivity. An adapted acid-tolerant Propionibacterium acidipropionici CGMCC 1.2230 strain was selected to produce propionic acid with a relatively high productivity (0.29 g/(Lh)) in the free-cell fermentation. Further immobilized-cell fermentation in fibrous-bed bioreactor (FBB) supported high-level repeated batch fermentations with a high productivity of 0.96 g/(Lh). The FBB also presents the potential to increase final propionic acid concentration by using glucose feeding strategy. The propionic acid concentration was increased to 51.2g/L in the fed-batch fermentation with the productivity of 0.71 g/(Lh). By adopting the above strategies, sugarcane bagasse hydrolysate could support the production of propionic acid with high productivity in the repeat-batch and fed-batch fermentations. The present work would pave one road to the accomplishment of large-scale bioproduction of propionic acid from renewable resources.
authors.affiliation: Institute of Biological Engineering, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China.
authors.forename: Linqi
authors.initials: L
authors.lastname: Zhu
authors.forename: Peilian
authors.initials: P
authors.lastname: Wei
authors.forename: Jin
authors.initials: J
authors.lastname: Cai
authors.forename: Xiangcheng
authors.initials: X
authors.lastname: Zhu
authors.forename: Zimeng
authors.initials: Z
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Huang
authors.forename: Zhinan
authors.initials: Z
authors.lastname: Xu
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects; D061249:Batch Cell Culture Techniques; D019149:Bioreactors / Q000382:microbiology*; D002244:Carbon / Q000494:pharmacology; D018914:Cells, Immobilized; D002482:Cellulose / Q000737:chemistry; D005285:Fermentation / Q000187:drug effects; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D006868:Hydrolysis / Q000187:drug effects; D007700:Kinetics; D011422:Propionates / Q000378:metabolism*; D011424:Propionibacterium / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D031786:Saccharum / Q000737:chemistry
chemical_list: D011422:Propionates; D002244:Carbon; D002482:Cellulose; C027433:bagasse; D005947:Glucose; C029658:propionic acid