title: Lymphocytes as internal standard in oxidative burst analysis by cytometry: a new data analysis approach.
abstract: We propose a new data analysis approach for reactive oxygen species detection using Dihydrorhodamine 123 in blood monocytes and neutrophils. This approach, based on data transformation using lymphocytes as internal standard, allows to appreciate free radical production by monocytes also without Phorbol 12-myristate 13-acetate (PMA) activation. In addition, this method is sensitive to differences in healthy subjects due to sub-pathological conditions, such as hypercholesterolemia.
authors.affiliation: Istituto Nazionale di Ricerca per gli Alimenti e la Nutrizione (INRAN), Rome, Italy. peluso@inran.it
authors.forename: Ilaria
authors.initials: I
authors.lastname: Peluso
authors.forename: Giuseppa
authors.initials: G
authors.lastname: Morabito
authors.forename: Silvia
authors.initials: S
authors.lastname: Riondino
authors.forename: Francesca
authors.initials: F
authors.lastname: La Farina
authors.forename: Mauro
authors.initials: M
authors.lastname: Serafini
mesh_terms: D000328:Adult; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*; D009504:Neutrophils / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D016897:Respiratory Burst; D013223:Statistics as Topic / Q000379:methods*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D017382:Reactive Oxygen Species; D013755:Tetradecanoylphorbol Acetate