title: The effect of nitrite inhibition on the anammox process.
abstract: The negative effect of nitrite on anammox activity has been reported widely during the past decade. Although the adverse effect is clear, conflicting reports exist on the level at which it occurs and its reversible/irreversible nature. An in depth study on nitrite inhibition therefore was performed in which the influence of environmental factors was evaluated. Anammox activity was measured in anammox granules by continuously monitored standardized manometric batch tests extending the interpretation by evaluation of lag times, maximum conversion rates during the tests and substrates/product conversion ratios. The granules where obtained from a one-stage anammox reactor, the dominant anammox organisms belonged to the Brocadia type. The observed 50% activity inhibition for nitrite (IC(50)) was 0.4 g N L(-1). The activity recovered fully after removal of the nitrite. Conversion in fresh medium after exposure to up to 6 g NO(2)(-)-N L(-1) for 24 h showed less then 60% loss of activity. Presence of ammonium during nitrite (2 g N L(-1)) exposure resulted in a stronger loss of activity after nitrite exposure (50% and 30% in presence and absence of ammonium respectively). Presence of oxygen during nitrite incubation led to a maximum activity reduction of 32%. The recovery after exposure indicates that the adverse effect of nitrite is reversible and thus inhibitory rather than toxic in nature. Similarities between exposure at three different pH-values indicate that nitrite rather than nitrous acid is the actual inhibiting compound.
authors.affiliation: Department of Biotechnology, Delft University of Technology, Julianalaan 67, Delft 2628 BC, the Netherlands. T.Lotti@tudelft.nl
authors.forename: T
authors.initials: T
authors.lastname: Lotti
authors.forename: W R L
authors.initials: WR
authors.lastname: van der Star
authors.forename: R
authors.initials: R
authors.lastname: Kleerebezem
authors.forename: C
authors.initials: C
authors.lastname: Lubello
authors.forename: M C M
authors.initials: MC
authors.lastname: van Loosdrecht
mesh_terms: D000332:Aerobiosis / Q000187:drug effects; D000693:Anaerobiosis / Q000187:drug effects; D018533:Biomass; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D008365:Manometry; D009573:Nitrites / Q000494:pharmacology*; D009584:Nitrogen / Q000032:analysis; D010084:Oxidation-Reduction / Q000187:drug effects; D000644:Quaternary Ammonium Compounds / Q000378:metabolism* / Q000494:pharmacology; D013997:Time Factors
chemical_list: D009573:Nitrites; D000644:Quaternary Ammonium Compounds; D009584:Nitrogen