title: A cyclodextrin host-guest recognition approach to a label-free electrochemical DNA hybridization biosensor.
abstract: A novel label-free electrochemical DNA hybridization biosensor using a β-cyclodextrin/poly(N-acetylaniline)/carbon nanotube composite modified screen printed electrode (CD/PNAANI/CNT/SPE) has been developed. The proposed DNA hybridization biosensor relies on the intrinsic oxidation signals of guanine (G) and adenine (A) from single-stranded DNA entered into the cyclodextrin (CD) cavity. Due to the binding of G and A bases to complementary cytosine and thymine bases in dsDNA, the signals obtained for ssDNA were much higher than that of dsDNA. The synergistic effect of the multi-walled carbon nanotubes provides a significantly enhanced voltammetric signal, and the CD encapsulation effect makes anodic peaks of G and A shift to less positive potentials than that at the bare SPE. The peak heights of G and A signals are dependent on both the number of the respective bases in oligonucleotides and the concentration of the target DNA sequences. Hybridization of complementary strands was monitored through the measurements of oxidation signal of purine bases, which enabled the detection of target sequences from 0.01 to 1.02 nmol μl(-1) with the detection limit of target DNA as low as 5.0 pmol μl(-1) (S/N = 3). Implementation of label-free and homogeneous electrochemical hybridization detection constitutes an important step toward low-cost, simple, highly sensitive and accurate DNA assay. Discrimination between complementary, noncomplementary, and two-base mismatch targets was easily accomplished using the proposed electrode.
authors.affiliation: Chemistry Department, College of Sciences, Shiraz University, Shiraz, 7145685464, Iran. abbaspour@chem.susc.ac.ir
authors.forename: Abdolkarim
authors.initials: A
authors.lastname: Abbaspour
authors.forename: Abolhassan
authors.initials: A
authors.lastname: Noori
mesh_terms: D001483:Base Sequence; D015374:Biosensing Techniques; D003505:Cyclodextrins / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D015342:DNA Probes; D004563:Electrochemistry / Q000295:instrumentation*; D006863:Hydrogen-Ion Concentration; D009693:Nucleic Acid Hybridization
chemical_list: D003505:Cyclodextrins; D015342:DNA Probes; D004247:DNA