title: Mesh-integrated microdroplet array for simultaneous merging and storage of single-cell droplets.
abstract: We constructed a mesh-grid integrated microwell array which enables easy trapping and consistent addition of droplets. The grid acts as a microchannel structure to guide droplets into the microwells underneath, and also provides open access for additional manipulation in a high-throughput manner. Each droplet in the array forms a stable environment of pico-litre volume to implement a single-cell-based assay.
authors.affiliation: Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), Yuseong-gu, Daejeon, Republic of Korea.
authors.forename: Eujin
authors.initials: E
authors.lastname: Um
authors.forename: Eugene
authors.initials: E
authors.lastname: Rha
authors.forename: Su-Lim
authors.initials: SL
authors.lastname: Choi
authors.forename: Seung-Goo
authors.initials: SG
authors.lastname: Lee
authors.forename: Je-Kyun
authors.initials: JK
authors.lastname: Park
mesh_terms: D018929:Cell Culture Techniques / Q000295:instrumentation; D002469:Cell Separation / Q000295:instrumentation; D004926:Escherichia coli / Q000737:chemistry / Q000302:isolation & purification; D049452:Green Fluorescent Proteins / Q000737:chemistry; D057166:High-Throughput Screening Assays / Q000295:instrumentation; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D059010:Single-Cell Analysis / Q000295:instrumentation*
chemical_list: D049452:Green Fluorescent Proteins