title: Modulatory effects of Beclin 1 on expression of angiopoietin and Tie-2 receptor in human cervical cancer cells.
abstract: OBJECTIVE
  To investigate the effect of Beclin 1, an autophagy gene, on the expression of angiopoietin (Ang) protein and the Tie-2 receptor in CaSki human cervical cancer cells.
  
  
  METHODS
  Beclin 1 overexpression (pcDNA3.1-Beclin1) and knockdown (pSUPER-Beclin1) plasmids were independently transfected into CaSki cells, and stably transfected cells were selected. Real-time fluorescence quantitative PCR and Western blot analyses were performed to detect the mRNA and protein expression of Beclin 1, Ang-1, Ang-2, and Tie-2. MTT assays were employed to determine cell proliferation rates.
  
  
  RESULTS
  In the cells transfected with pcDNA3.1-Beclin1, the expression of Beclin 1, Ang-2, and Tie-2 was markedly increased, but expression of Ang-1 was dramatically reduced. MTT assays revealed that the proliferation of these cells was also significantly suppressed. In the CaSki cells transfected with pSUPER-Beclin1, the expression of Beclin 1, Ang-2, and Tie-2 was inhibited.
  
  
  CONCLUSIONS
  Overexpression of Beclin 1 can inhibit the proliferation of CaSki cells, which may be attributed to an imbalance among the expression of Ang-1, Ang-2 and Tie-2.
authors.affiliation: Department of Obstetrics and Gynecology, Fujian Provincial Hospital, Fujian Provincial Clinical Medical College, Fujian Medical University, Fuzhou, China. garyhanyu@sina.com
authors.forename: Yang
authors.initials: Y
authors.lastname: Sun
authors.forename: Jia-hua
authors.initials: JH
authors.lastname: Liu
authors.forename: Ling
authors.initials: L
authors.lastname: Pan
authors.forename: Long
authors.initials: L
authors.lastname: Jin
authors.forename: Yin
authors.initials: Y
authors.lastname: Yang
authors.forename: Yu-xia
authors.initials: YX
authors.lastname: Sui
authors.forename: Hong
authors.initials: H
authors.lastname: Shi
mesh_terms: D042683:Angiopoietin-1 / Q000378:metabolism*; D042702:Angiopoietin-2 / Q000378:metabolism*; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism*; D001343:Autophagy / Q000235:genetics; D000071186:Beclin-1; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D042787:Receptor, TIE-2 / Q000378:metabolism*; D014162:Transfection; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
chemical_list: D042683:Angiopoietin-1; D042702:Angiopoietin-2; D051017:Apoptosis Regulatory Proteins; C491997:BECN1 protein, human; D000071186:Beclin-1; D008565:Membrane Proteins; D012333:RNA, Messenger; D042787:Receptor, TIE-2