title: Expression of carbonic anhydrases I/II and the correlation to clinical aspects of oral squamous cell carcinoma analyzed using tissue microarray.
abstract: BACKGROUND
  Carbonic anhydrases (CA), a family of metalloenzymes, play an important role in catalyzing the equilibration of carbon dioxide (CO(2)) and carbonic acid (H(2)CO(3)). The role of CAs in tumorigenesis is controversial, especially regarding the expression of CA isoenzymes between various tumor types. This study explores the correlation between the expressions of CA I and CA II and the characteristic features of oral cancer.
  
  
  METHODS
  We immunohistochemically examined the expressions of CA I and CA II in 279 cases of oral squamous cell carcinoma (OSCC) using tissue microarrays. Additionally, the oral cancer cell line SCC-9 was used to confirm the relationship between CA I and CA II expression and cell growth.
  
  
  RESULTS
  We found a significant correlation between positive CA I and CA II stains and OSCC for more advanced clinical stage (P = 0.014 or 0.012) and larger tumor size (P = 0.008 or 0.038), but not for positive lymph node metastasis, distal metastasis, and recurrence. In vitro analysis also showed that treatment with a CA inhibitor, acetazolamide, inhibited the growth of SCC-9 cells.
  
  
  CONCLUSIONS
  We conclude that expressions of CA I and CA II in OSCC samples can be used to predict local tumor growth in OSCC patients.
authors.affiliation: School of Dentistry, Chung Shan Medical University, Taichung, Taiwan.
authors.forename: Chia-Ming
authors.initials: CM
authors.lastname: Liu
authors.forename: Yueh-Min
authors.initials: YM
authors.lastname: Lin
authors.forename: Kun-Tu
authors.initials: KT
authors.lastname: Yeh
authors.forename: Mu-Kuan
authors.initials: MK
authors.lastname: Chen
authors.forename: Jer-Hwa
authors.initials: JH
authors.lastname: Chang
authors.forename: Chih-Jung
authors.initials: CJ
authors.lastname: Chen
authors.forename: Ming-Yung
authors.initials: MY
authors.lastname: Chou
authors.forename: Shun-Fa
authors.initials: SF
authors.lastname: Yang
authors.forename: Ming-Hsien
authors.initials: MH
authors.lastname: Chien
mesh_terms: D000328:Adult; D000368:Aged; D024401:Carbonic Anhydrase I / Q000378:metabolism*; D024402:Carbonic Anhydrase II / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000201:enzymology* / Q000473:pathology; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000201:enzymology* / Q000473:pathology; D009363:Neoplasm Proteins / Q000378:metabolism*; D046888:Tissue Array Analysis
chemical_list: D009363:Neoplasm Proteins; D024401:Carbonic Anhydrase I; D024402:Carbonic Anhydrase II