title: In vitro activation of human leukocytes in response to contact with synthetic hernia meshes.
abstract: OBJECTIVE
  Evaluation of an in vitro chemiluminescent screen to predict leukocyte ROS in response to surgical materials.
  
  
  METHODS
  6 surgical meshes; manufacture and knitting variations of polypropylene (PP), polyester terephtalate (PET) and polyglycolic acid (PGA) trialled healthy human blood (n=5). Materials and blood were incubated with pholasin. Pholasin emits photons in the presence of reactive oxygen species; secreted by activated leukocytes.
  
  
  RESULTS
  Multifilament-PGA mesh stimulated the greatest ROS response from blood derived human leukocytes. Multifilament-PET light weight and multifilament-PP meshes stimulated similar levels of ROS production which were greater than monofilament-PP light, monofilament-PP and monofilament-PET light meshes. Data demonstrated statistical variations in trans-donor response to the materials.
  
  
  CONCLUSIONS
  An in vitro chemiluminescent assay can be used to assess leukocyte respiratory burst response to biomaterials. PGA mesh elicited the greatest ROS response. PP and PET monofilament meshes induce less ROS than multifilament equivalents. In vitro results correlate with previously published clinical responses to these materials.
authors.affiliation: Clinical Engineering, UKCTE, UKBioTEC, The Institute of Ageing and Chronic Disease, University of Liverpool, Duncan Building, Daulby Street, Liverpool, L69 3GA, UK. n.bryan@liv.ac.uk
authors.forename: N
authors.initials: N
authors.lastname: Bryan
authors.forename: H
authors.initials: H
authors.lastname: Ahswin
authors.forename: N J
authors.initials: NJ
authors.lastname: Smart
authors.forename: Y
authors.initials: Y
authors.lastname: Bayon
authors.forename: J A
authors.initials: JA
authors.lastname: Hunt
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D049454:Firefly Luciferin; D006801:Humans; D007962:Leukocytes / Q000187:drug effects* / Q000378:metabolism; D008163:Luminescent Measurements; D008422:Materials Testing; D017785:Photons; D010795:Phthalic Acids; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology*; D011100:Polyglycolic Acid / Q000737:chemistry / Q000494:pharmacology*; D011126:Polypropylenes / Q000737:chemistry / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013526:Surgical Mesh / Q000009:adverse effects*
chemical_list: D001672:Biocompatible Materials; D010795:Phthalic Acids; D011091:Polyesters; D011126:Polypropylenes; D017382:Reactive Oxygen Species; D011100:Polyglycolic Acid; D049454:Firefly Luciferin; C011363:terephthalic acid; C052157:pholasin