title: Preparation of mouse monoclonal antibody for RB1CC1 and its clinical application.
abstract: RB1-inducible coiled-coil 1 (RB1CC1; also known as FIP200) plays important roles in several biological pathways such as cell proliferation and autophagy. Evaluation of RB1CC1 expression can provide useful clinical information on various cancers and neurodegenerative diseases. In order to realize the clinical applications, it is necessary to establish a stable supply of antibody and reproducible procedures for the laboratory examinations. In the present study, we have generated mouse monoclonal antibodies for RB1CC1, and four kinds of antibodies (N1-8, N1-216, N3-2, and N3-42) were found to be optimal for clinical applications such as ELISA and immunoblots and work as well as the pre-existing polyclonal antibodies. N1-8 monoclonal antibody provided the best recognition of RB1CC1 in the clinico-pathological examination of formalin-fixed paraffin-embedded tissues. These monoclonal antibodies will help to generate new opportunities in scientific examinations in biology and clinical medicine.
authors.affiliation: Department of Clinical Laboratory Medicine, Shiga University of Medical Science, Shiga, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Hama
authors.forename: Tokuhiro
authors.initials: T
authors.lastname: Chano
authors.forename: Takuma
authors.initials: T
authors.lastname: Inui
authors.forename: Kyoichi
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Okabe
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D000071183:Autophagy-Related Proteins; D001943:Breast Neoplasms / Q000378:metabolism; D045744:Cell Line, Tumor; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D018604:Epitope Mapping; D005260:Female; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry / Q000379:methods; D051379:Mice; D011379:Prognosis; D017434:Protein Structure, Tertiary; D011505:Protein-Tyrosine Kinases / Q000737:chemistry*
chemical_list: D000911:Antibodies, Monoclonal; D000071183:Autophagy-Related Proteins; C407704:RB1CC1 protein, human; D011505:Protein-Tyrosine Kinases