title: Histological and immunohistochemical analyses of the chronology of healing process after immediate tooth replantation in incisor rat teeth.
abstract: Dental tissues have special characteristics, and its regenerative capacity is noteworthy. However, understanding the circumstances that lead to regeneration is challenging. In this study, the chronology of the healing process after immediate replantation of rat incisor teeth was examined by histological and immunohistochemical analyses within a 60-day period. Thirty-six male Wistar rats had their maxillary right incisors extracted and replanted after 15 min in saline storage. The rats were sacrificed immediately 3, 7, 15, 28, and 60 days after replantation. The histological analysis showed rupture of the periodontal ligament and formation of a blood clot, which started being replaced by a connective tissue after 3 days. At 7 days, the gingival mucosa epithelium was reinserted and areas of root resorption could be seen. At 15 days, the periodontal ligament was repaired. At 3 days, the pulp presented an absence of the odontoblast layer, which started being replaced by a connective tissue. This tissue suffered gradual calcification, filling the root canal at 28 and 60 days. The root ends were closed. The immunohistochemical analysis revealed greater expression of OP, OPG, and RANK proteins in the initial periods (0 and 3 days), while TRAP expression predominated at 28 and 60 days (P < 0.05). In conclusion, in delayed tooth replantation, there is great new bone formation activity in the earlier periods of the repair process, while a predominance of bone resorption and remodeling is observed in the more advanced periods.
authors.affiliation: Department of Surgery and Integrated Clinic, Araçatuba Dental School, UNESP - Univ Estadual Paulista, Araçatuba, SP, Brazil.
authors.forename: Sônia Regina
authors.initials: SR
authors.lastname: Panzarini
authors.forename: Roberta
authors.initials: R
authors.lastname: Okamoto
authors.forename: Wilson Roberto
authors.initials: WR
authors.lastname: Poi
authors.forename: Celso Koogi
authors.initials: CK
authors.lastname: Sonoda
authors.forename: Denise
authors.initials: D
authors.lastname: Pedrini
authors.forename: Paula Ervolino
authors.initials: PE
authors.lastname: da Silva
authors.forename: Celia Tomiko Matida Hamata
authors.initials: CT
authors.lastname: Saito
authors.forename: Heloísa Fonseca
authors.initials: HF
authors.lastname: Marão
authors.forename: Paulo
authors.initials: P
authors.lastname: Sedlacek
mesh_terms: D000135:Acid Phosphatase / Q000032:analysis; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D001777:Blood Coagulation / Q000502:physiology; D003094:Collagen; D003238:Connective Tissue / Q000473:pathology; D003782:Dental Pulp / Q000473:pathology; D003784:Dental Pulp Calcification / Q000209:etiology / Q000473:pathology; D004848:Epithelium / Q000473:pathology; D005881:Gingiva / Q000473:pathology; D007150:Immunohistochemistry; D007180:Incisor / Q000293:injuries*; D007527:Isoenzymes / Q000032:analysis; D008297:Male; D009804:Odontoblasts / Q000473:pathology; D053495:Osteopontin / Q000032:analysis; D053244:Osteoprotegerin / Q000032:analysis; D010513:Periodontal Ligament / Q000293:injuries / Q000473:pathology; D053245:RANK Ligand / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D053246:Receptor Activator of Nuclear Factor-kappa B / Q000032:analysis; D012391:Root Resorption / Q000209:etiology / Q000473:pathology; D012421:Rupture; D000071681:Tartrate-Resistant Acid Phosphatase; D013997:Time Factors; D019227:Tooth Apex / Q000473:pathology; D014090:Tooth Replantation / Q000379:methods*; D020390:Tooth Socket / Q000473:pathology; D014945:Wound Healing / Q000502:physiology
chemical_list: D015415:Biomarkers; D007527:Isoenzymes; D053244:Osteoprotegerin; D053245:RANK Ligand; D053246:Receptor Activator of Nuclear Factor-kappa B; C506897:Spp1 protein, rat; C506032:Tnfrsf11b protein, rat; D053495:Osteopontin; D003094:Collagen; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase