title: Microarray analysis of Etrog citron (Citrus medica L.) reveals changes in chloroplast, cell wall, peroxidase and symporter activities in response to viroid infection.
abstract: Viroids are small (246-401 nucleotides), single-stranded, circular RNA molecules that infect several crop plants and can cause diseases of economic importance. Citrus are the hosts in which the largest number of viroids have been identified. Citrus exocortis viroid (CEVd), the causal agent of citrus exocortis disease, induces considerable losses in citrus crops. Changes in the gene expression profile during the early (pre-symptomatic) and late (post-symptomatic) stages of Etrog citron infected with CEVd were investigated using a citrus cDNA microarray. MaSigPro analysis was performed and, on the basis of gene expression profiles as a function of the time after infection, the differentially expressed genes were classified into five clusters. FatiScan analysis revealed significant enrichment of functional categories for each cluster, indicating that viroid infection triggers important changes in chloroplast, cell wall, peroxidase and symporter activities.
authors.affiliation: Department of Phytosanitary Sciences and Technologies-University of Catania, Via S. Sofia 102, 95123 Catania, Italy.
authors.forename: Serena
authors.initials: S
authors.lastname: Rizza
authors.forename: Ana
authors.initials: A
authors.lastname: Conesa
authors.forename: José
authors.initials: J
authors.lastname: Juarez
authors.forename: Antonino
authors.initials: A
authors.lastname: Catara
authors.forename: Luis
authors.initials: L
authors.lastname: Navarro
authors.forename: Nuria
authors.initials: N
authors.lastname: Duran-Vila
authors.forename: Gema
authors.initials: G
authors.lastname: Ancillo
mesh_terms: D001483:Base Sequence; D002473:Cell Wall / Q000378:metabolism*; D002736:Chloroplasts / Q000378:metabolism*; D002957:Citrus / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D017931:DNA Primers; D020411:Oligonucleotide Array Sequence Analysis; D010544:Peroxidases / Q000378:metabolism*; D027981:Symporters / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D027981:Symporters; D010544:Peroxidases