title: [Preparation and characterization of monoclonal antibody against Vibrio vulnificus].
abstract: OBJECTIVE
  To prepare high-performance and specific monoclonal antibody (mAb) against Vibrio vulnificus and carry out characterization.
  
  
  METHODS
  BALB/c mice were immunized with Vibrio vulnificus protein, and hybridoma cells against Vibrio vulnificus were produced by cellsion technique. The titers of mAbs against vvhA and cross-reaction between the anti-vvhA mAb and other other important marine bacteria were screened by ELISA and Western blot.
  
  
  RESULTS
  Five strains of hybridoma were obtained. Identification result indicated that 5 mAbs have favourable specifity and immunoreactivity.
  
  
  CONCLUSIONS
  Specific mAbs against Vibrio vulnificus were produced which provides an important preparation for establishing rapid-detection kit of detecting Vibrio vulnificus.
authors.affiliation: Clinical Laboratory, Navy General Hospital, Beijing, China. fukaifei@sina.com
authors.forename: Kai-fei
authors.initials: KF
authors.lastname: Fu
authors.forename: Cong
authors.initials: C
authors.lastname: Ma
authors.forename: Jian-wei
authors.initials: JW
authors.lastname: Guo
authors.forename: Yuan-yuan
authors.initials: YY
authors.lastname: Qiao
authors.forename: Xiu-hong
authors.initials: XH
authors.lastname: Hao
authors.forename: Da-peng
authors.initials: DP
authors.lastname: Wang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification*; D000918:Antibody Specificity; D001426:Bacterial Proteins / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006825:Hybridomas / Q000378:metabolism; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D041261:Vibrio vulnificus / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins