title: Immobilization of Pholiota adiposa xylanase onto SiO₂ nanoparticles and its application for production of xylooligosaccharides.
abstract: Enhanced yields of different lignocellulases were obtained under statistically-optimized parameters using Pholiota adiposa. The k (cat) value (4,261 s(-1)) of purified xylanase under standard assay conditions was the highest value ever reported. On covalent immobilization of the crude xylanase preparation onto functionalized silicon oxide nanoparticles, 66 % of the loaded enzyme was retained on the particle. Immobilized enzyme gave 45 % higher concentrations of xylooligosaccharides compared to the free enzyme. After 17 cycles, the immobilized enzyme retained 97 % of the original activity, demonstrating its prospects for the synthesis of xylooligosaccharides in industrial applications.
authors.affiliation: Department of Chemical Engineering, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul 143-701, Republic of Korea.
authors.forename: Saurabh Sudha
authors.initials: SS
authors.lastname: Dhiman
authors.forename: Sujit Sadashiv
authors.initials: SS
authors.lastname: Jagtap
authors.forename: Marimuthu
authors.initials: M
authors.lastname: Jeya
authors.forename: Jung-Rim
authors.initials: JR
authors.lastname: Haw
authors.forename: Yun Chan
authors.initials: YC
authors.lastname: Kang
authors.forename: Jung-Kul
authors.initials: JK
authors.lastname: Lee
mesh_terms: D004800:Enzymes, Immobilized / Q000378:metabolism*; D053758:Nanoparticles; D009844:Oligosaccharides / Q000378:metabolism*; D055368:Pholiota / Q000201:enzymology*; D011485:Protein Binding; D012822:Silicon Dioxide / Q000378:metabolism*; D014995:Xylosidases / Q000378:metabolism*
chemical_list: D004800:Enzymes, Immobilized; D009844:Oligosaccharides; D012822:Silicon Dioxide; D014995:Xylosidases