title: Effect of quercetin on Vibrio cholerae induced nuclear factor-κB activation and interleukin-8 expression in intestinal epithelial cells.
abstract: Vibrio cholerae, the etiological agent of cholera, colonizes the small intestine, produces an enterotoxin and causes acute inflammatory response at intestinal epithelial cell surface. Pretreatment of intestinal epithelial cells with quercetin reduces the level of V. cholerae induced IL-8 in dose and time dependent manner as determined by ELISA and RT-PCR. Immunofluorescence studies showed that quercetin suppresses the translocation of p50 subunit of NF-κB. In vivo, quercetin administration produced a significant reduction of neutrophil infiltration in the intestinal epithelial layer of suckling mouse. Taken together, quercetin could inhibit the V. cholerae induced inflammation and may therefore find use in management of V. cholerae induced pathogenesis.
authors.affiliation: Molecular & Human Genetics Division, Indian Institute of Chemical Biology-Unit of CSIR, 4 Raja S.C. Mullick Road, Kolkata 700032, India.
authors.forename: Tapasi
authors.initials: T
authors.lastname: Das
authors.forename: Sanjit
authors.initials: S
authors.lastname: Mukherjee
authors.forename: Keya
authors.initials: K
authors.lastname: Chaudhuri
mesh_terms: D000818:Animals; D000833:Animals, Suckling; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000187:drug effects / Q000276:immunology* / Q000382:microbiology*; D016209:Interleukin-8 / Q000096:biosynthesis*; D007421:Intestine, Small / Q000276:immunology / Q000473:pathology; D007962:Leukocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence; D016328:NF-kappa B / Q000378:metabolism*; D011794:Quercetin / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D014734:Vibrio cholerae / Q000276:immunology*
chemical_list: D000893:Anti-Inflammatory Agents; D016209:Interleukin-8; D016328:NF-kappa B; D011794:Quercetin