title: Isolation, identification and antimicrobial susceptibility of Bacteroides fragilis group strains recovered from broiler faeces.
abstract: 1. The objective was to evaluate the occurrence of cultivable components of the Bacteroides fragilis group in faeces of broiler chickens and their antimicrobial susceptibility patterns.   2. Faecal samples of 36 × 45-d-old Cobb broilers of both sexes from 15 different flocks on one farm were diluted 10-fold and plated on to Bacteroides-bile-esculin agar for colony count and isolation. Identification was by molecular methods and antimicrobial susceptibility in the agar dilution assay.   3. A total of 236 isolates was recovered from a mean population of 3·32 × 10(7 )colony-forming units/g of faeces. B. fragilis was shown to be the predominant Bacteroides species (45·3%), followed by B. distasonis (35·6%), B. vulgatus (8·9%), B. ovatus (2·5%) and B. stercoris (1·3%).   4. Among 204 bacterial isolates tested, high resistance to ampicillin (98·5%), norfloxacin (95·1%) and tetracycline (88·2%) were observed. High (89·7%) multi-drug resistance was observed to 3-7 of the tested drugs.   5. Components of the B. fragilis group were sub-dominant in broiler faecal microbiota, with a different species pattern compared with human and high antimicrobial multi-drug resistance.
authors.affiliation: Departamento de Microbiologia, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: G D
authors.initials: GD
authors.lastname: Garcia
authors.forename: M A R
authors.initials: MA
authors.lastname: Carvalho
authors.forename: C G
authors.initials: CG
authors.lastname: Diniz
authors.forename: J L
authors.initials: JL
authors.lastname: Marques
authors.forename: J R
authors.initials: JR
authors.lastname: Nicoli
authors.forename: L M
authors.initials: LM
authors.lastname: Farias
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001439:Bacteroides / Q000145:classification* / Q000187:drug effects* / Q000302:isolation & purification; D002645:Chickens; D024901:Drug Resistance, Multiple, Bacterial; D005243:Feces / Q000382:microbiology*; D005260:Female; D008297:Male
chemical_list: D000900:Anti-Bacterial Agents