title: Identification, purification and characterization of laterosporulin, a novel bacteriocin produced by Brevibacillus sp. strain GI-9.
abstract: BACKGROUND
  Bacteriocins are antimicrobial peptides that are produced by bacteria as a defense mechanism in complex environments. Identification and characterization of novel bacteriocins in novel strains of bacteria is one of the important fields in bacteriology.
  
  
  RESULTS
  The strain GI-9 was identified as Brevibacillus sp. by 16 S rRNA gene sequence analysis. The bacteriocin produced by strain GI-9, namely, laterosporulin was purified from supernatant of the culture grown under optimal conditions using hydrophobic interaction chromatography and reverse-phase HPLC. The bacteriocin was active against a wide range of Gram-positive and Gram-negative bacteria. MALDI-TOF experiments determined the precise molecular mass of the peptide to be of 5.6 kDa and N-terminal sequencing of the thermo-stable peptide revealed low similarity with existing antimicrobial peptides. The putative open reading frame (ORF) encoding laterosporulin and its surrounding genomic region was fished out from the draft genome sequence of GI-9. Sequence analysis of the putative bacteriocin gene did not show significant similarity to any reported bacteriocin producing genes in database.
  
  
  CONCLUSIONS
  We have identified a bacteriocin producing strain GI-9, belonging to the genus Brevibacillus sp. Biochemical and genomic characterization of laterosporulin suggests it as a novel bacteriocin with broad spectrum antibacterial activity.
authors.affiliation: Council of Scientific and Industrial Research-Institute of Microbial Technology, Chandigarh, India.
authors.forename: Pradip Kumar
authors.initials: PK
authors.lastname: Singh
authors.lastname: Chittpurna
authors.lastname: Ashish
authors.forename: Vikas
authors.initials: V
authors.lastname: Sharma
authors.forename: Prabhu B
authors.initials: PB
authors.lastname: Patil
authors.forename: Suresh
authors.initials: S
authors.lastname: Korpole
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001430:Bacteriocins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D058675:Brevibacillus / Q000235:genetics / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D008970:Molecular Weight; D005810:Multigene Family / Q000235:genetics; D010641:Phenotype; D055550:Protein Stability; D059748:Proteolysis; D017421:Sequence Analysis; D013696:Temperature
chemical_list: D000900:Anti-Bacterial Agents; D001430:Bacteriocins