title: Damping factor links periodic focusing and uniform field ion mobility for accurate determination of collision cross sections.
abstract: The methodology for obtaining accurate ion-neutral collision cross section (Ω) values for peptides and proteins using periodic focusing ion mobility spectrometry (PF IMS) is presented. A mobility dampening factor (represented by the term α) is introduced to account for the relative increase in ion-neutral collisions in PF IMS compared to uniform field ion mobility spectrometry (UF IMS) for equivalent operating conditions. The results show that α may be easily quantified both theoretically and empirically for a specific PF IMS design operating at a given pressure based upon the charge state of the analyte. By simply incorporating an α term into traditional UF IMS expressions, accurate Ω values were obtained with excellent agreement (≤4% difference) compared to UF IMS measurements found in the current literature.
authors.affiliation: Texas A&M University, Department of Chemistry, Laboratory for Biological Mass Spectrometry, College Station, Texas 77843, United States.
authors.forename: Joshua A
authors.initials: JA
authors.lastname: Silveira
authors.forename: Junho
authors.initials: J
authors.lastname: Jeon
authors.forename: Chaminda M
authors.initials: CM
authors.lastname: Gamage
authors.forename: Pei-Jing
authors.initials: PJ
authors.lastname: Pai
authors.forename: Kyle L
authors.initials: KL
authors.lastname: Fort
authors.forename: David H
authors.initials: DH
authors.lastname: Russell
mesh_terms: D000818:Animals; D002417:Cattle; D006736:Horses; D007477:Ions / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D010455:Peptides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*
chemical_list: D007477:Ions; D010455:Peptides; D011506:Proteins