title: ACE gene insertion/deletion polymorphism seminal associations in infertile men.
abstract: OBJECTIVE
  We assessed seminal associations of the ACE gene insertion/deletion polymorphism in infertile men.
  
  
  METHODS
  A total of 405 men were investigated, divided into healthy fertile men, and those with asthenozoospermia, asthenoteratozoospermia and oligoasthenoteratozoospermia, respectively. They underwent semen analysis, and assessment of sperm acrosin activity, hypo-osmotic swelling, seminal 8-iso-prostaglandin-F(2α), total antioxidant capacity, α-glucosidase and ACE gene polymorphisms.
  
  
  RESULTS
  The ACE insertion/insertion genotype was noted in 182 men, including 76.5% of healthy fertile men, and 47.4%, 39.8% and 17.6% of those with asthenozoospermia, asthenoteratozoospermia and oligoasthenoteratozoospermia, respectively. The ACE insertion/deletion genotype was noted in 133 men, including 13.7% of healthy fertile men, and 42.3%, 27.5% and 47.2% of those with asthenozoospermia, asthenoteratozoospermia and oligoasthenoteratozoospermia, respectively. The ACE deletion/deletion genotype was identified in 90 men, including 9.8% of healthy fertile men, 10.3%, 32.70% and 35.2% of those with asthenozoospermia, asthenoteratozoospermia and oligoasthenoteratozoospermia, respectively. Men with the ACE deletion/deletion and insertion/deletion genotypes showed a significant decrease in sperm count, motility, linear velocity and normal forms, acrosin activity index, hypo-osmotic swelling test and seminal α-glucosidase, and significantly increased seminal 8-iso-prostaglandin-F(2α) than those with the ACE insertion/insertion genotype.
  
  
  CONCLUSIONS
  ACE gene deletion polymorphism is associated with abnormal seminal variables, such that carriers of the ACE deletion/deletion genotype have higher seminal oxidative stress.
authors.affiliation: Department of Medical Biochemistry, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
authors.forename: Adel A
authors.initials: AA
authors.lastname: Zalata
authors.forename: Heba K
authors.initials: HK
authors.lastname: Morsy
authors.forename: Abd El-Naser
authors.initials: Ael-N
authors.lastname: Badawy
authors.forename: Samir
authors.initials: S
authors.lastname: Elhanbly
authors.forename: Taymour
authors.initials: T
authors.lastname: Mostafa
mesh_terms: D017353:Gene Deletion; D006801:Humans; D007248:Infertility, Male / Q000235:genetics*; D008297:Male; D016254:Mutagenesis, Insertional; D018384:Oxidative Stress; D007703:Peptidyl-Dipeptidase A / Q000235:genetics*; D011110:Polymorphism, Genetic; D012661:Semen / Q000378:metabolism; D013076:Sperm Count; D013081:Sperm Motility
chemical_list: C488034:ACE protein, human; D007703:Peptidyl-Dipeptidase A