title: The CBFA2T3/ACSF3 locus is recurrently involved in IGH chromosomal  translocation t(14;16)(q32;q24) in pediatric B-cell lymphoma with germinal center phenotype.
abstract: Translocations involving immunoglobulin (IG) loci are the hallmarks of several subtypes of B-cell lymphoma. Common to these translocations is that cellular proto-oncogenes come under the influence of IG regulatory elements leading to deregulated expression. In case of a breakpoint in the IGH switch region, oncogene activation can take place on both derivative chromosomes, which means that in principle one translocation can result in concurrent activation of two genes. By fluorescence in situ hybridization (FISH), we identified a case of leukemic B-cell lymphoma in a child with an IGH break and unknown partner. Subsequent long-distance inverse PCR revealed fusion of IGH Sl in 14q32 and the 50 region of CBFA2T3 in 16q24.3, suggesting presence of the t(14;16)(q32;q24.3). Candidate oncogenes targeted through this translocation are CBFA2T3 and ACSF3, which could be activated on der(16) and der(14), respectively. FISH screening of a population-based cohort of B-cell lymphomas from a prospective trial for the treatment of lymphoma in childhood (BFM-NHL) identified additionally a follicular lymphoma Grade 3/diffuse large B-cell lymphoma with IGH-CBFA2T3/ACSF3 juxtaposition. Both lymphomas shared expression of CD10 and CD20 in the absence of TdT, suggesting a germinal center (GC) B-cell origin. Our data indicate that the CBFA2T3/ACSF3 locus is a novel recurrent oncogenic target of IGH translocations, which might contribute to the pathogenesis of pediatric GC-derived B-cell lymphoma.
authors.affiliation: Institute of Human Genetics, University Hospital Schleswig-Holstein Campus Kiel/Christian-Albrechts University, Kiel, Germany.
authors.forename: Itziar
authors.initials: I
authors.lastname: Salaverria
authors.forename: Takashi
authors.initials: T
authors.lastname: Akasaka
authors.forename: Stefan
authors.initials: S
authors.lastname: Gesk
authors.forename: Monika
authors.initials: M
authors.lastname: Szczepanowski
authors.forename: Birgit
authors.initials: B
authors.lastname: Burkhardt
authors.forename: Lana
authors.initials: L
authors.lastname: Harder
authors.forename: Christine
authors.initials: C
authors.lastname: Damm-Welk
authors.forename: Ilske
authors.initials: I
authors.lastname: Oschlies
authors.forename: Wolfram
authors.initials: W
authors.lastname: Klapper
authors.forename: Martin J S
authors.initials: MJ
authors.lastname: Dyer
authors.forename: Reiner
authors.initials: R
authors.lastname: Siebert
mesh_terms: D000293:Adolescent; D018951:Antigens, CD20 / Q000096:biosynthesis; D002648:Child; D002883:Chromosomes, Human, Pair 14 / Q000235:genetics; D002885:Chromosomes, Human, Pair 16 / Q000235:genetics; D003066:Coenzyme A Ligases / Q000235:genetics*; D005260:Female; D050438:Genes, Immunoglobulin Heavy Chain; D018858:Germinal Center / Q000473:pathology / Q000502:physiology; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D016393:Lymphoma, B-Cell / Q000235:genetics* / Q000473:pathology; D008224:Lymphoma, Follicular / Q000235:genetics; D008297:Male; D015260:Neprilysin / Q000096:biosynthesis; D012097:Repressor Proteins / Q000235:genetics*; D014178:Translocation, Genetic; D025521:Tumor Suppressor Proteins / Q000235:genetics*
chemical_list: D018951:Antigens, CD20; C115744:CBFA2T3 protein, human; D012097:Repressor Proteins; D025521:Tumor Suppressor Proteins; D015260:Neprilysin; C561770:ACSF3 protein, human; D003066:Coenzyme A Ligases