title: Surface display of human serum albumin on Bacillus subtilis spores for oral administration.
abstract: Human serum albumin (HSA) is the major protein component of human plasma. To date, HSA for clinical uses is mostly produced by fractionation of human whole blood, which is accompanied by a lot of limitations. To obtain long-term bioactive albumin, we used hsa as a foreign gene and constructed a recombinant plasmid pJS700-HSA which carries a recombinant gene cotC-hsa under the control of cotC promoter. Plasmid pJS700-HSA was transformed into Bacillus subtilis by double cross-over and an amylase inactivated mutant was produced. After induction of spore formation, western blot and fluorescence immunoassay were used to monitor HSA surface expression on spores. We estimated that HSA displayed on the spore accounted for 0.135 % of the total spore proteins and about 0.023 fg HSA were exposed on the surface of each spore. Oral administration to mice with spores displaying HSA implied that the recombinant spores may have potential ability to increase the serum albumin level in vivo due to the resistant characters of spores.
authors.affiliation: Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, Jiangsu, People's Republic of China.
authors.forename: Langyong
authors.initials: L
authors.lastname: Mao
authors.forename: Shantong
authors.initials: S
authors.lastname: Jiang
authors.forename: Guohui
authors.initials: G
authors.lastname: Li
authors.forename: Yuanqing
authors.initials: Y
authors.lastname: He
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
authors.forename: Qin
authors.initials: Q
authors.lastname: Yao
authors.forename: Keping
authors.initials: K
authors.lastname: Chen
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001412:Bacillus subtilis / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012709:Serum Albumin / Q000235:genetics / Q000378:metabolism*; D000075462:Serum Albumin, Human; D013171:Spores, Bacterial / Q000235:genetics / Q000378:metabolism*
chemical_list: C422455:ALB protein, human; D011994:Recombinant Proteins; D012709:Serum Albumin; D000075462:Serum Albumin, Human