title: Cytokines profiling by multiplex analysis in experimental arthritis: which pathophysiological relevance for articular versus systemic mediators?
abstract: BACKGROUND
  We have taken advantage of the large screening capacity of a multiplex immunoassay to better define the respective contribution of articular versus systemic cytokines in experimental arthritis.
  
  
  METHODS
  We performed a follow up (from 7 hours to 14 days) multiplex analysis of 24 cytokines in synovial fluid and sera of rats developing Antigen-Induced Arthritis (AIA) and confronted their protein level changes with molecular, biochemical, histological and clinical events occurring in the course of the disease.
  
  
  RESULTS
  The time-scheduled findings in arthritic joints correlated with time-dependent changes of cytokine amounts in joint effusions but not with their blood levels. From seven hours after sensitization, high levels of chemokines (MCP-1, MIP1α, GRO/KC, RANTES, eotaxin) were found in synovial fluid of arthritic knees whereas perivascular infiltration occurred in the synovium; local release of inflammatory cytokines (IFNγ, IL-1β, IL-6) preceded the spreading of inflammation and resulted in progressive degradation of cartilage and bone. Finally a local overexpression of several cytokines/adipocytokines poorly described in arthritis (IL-13, IL-18, leptin) was observed.
  
  
  CONCLUSIONS
  Distinct panels of cytokines were found in arthritic fluid during AIA, and the expected effect of mediators correlated well with changes occurring in joint tissues. Moreover, multiplex analysis could be helpful to identify new pathogenic mediators and to elucidate the mechanisms supporting the efficacy of putative targeted therapies.
authors.affiliation: Physiopathologie, Pharmacologie et Ingénierie Articulaire - PPIA-UMR 7561 CNRS UHP, Université de Lorraine, Faculté de Médecine, BP 184, 54505 Vandoeuvre Les Nancy, France.
authors.forename: Joseph
authors.initials: J
authors.lastname: Paquet
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Goebel
authors.forename: Camille
authors.initials: C
authors.lastname: Delaunay
authors.forename: Astrid
authors.initials: A
authors.lastname: Pinzano
authors.forename: Laurent
authors.initials: L
authors.lastname: Grossin
authors.forename: Christel
authors.initials: C
authors.lastname: Cournil-Henrionnet
authors.forename: Pierre
authors.initials: P
authors.lastname: Gillet
authors.forename: Patrick
authors.initials: P
authors.lastname: Netter
authors.forename: Jean-Yves
authors.initials: JY
authors.lastname: Jouzeau
authors.forename: David
authors.initials: D
authors.lastname: Moulin
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology*; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D002358:Cartilage, Articular / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology*; D016207:Cytokines / Q000097:blood / Q000378:metabolism*; D018836:Inflammation Mediators / Q000502:physiology*; D007596:Joints / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013582:Synovial Fluid / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D016207:Cytokines; D018836:Inflammation Mediators