title: Immobilization of HRP in Mesoporous Silica and Its Application for the Construction of Polyaniline Modified Hydrogen Peroxide Biosensor.
abstract: Polyaniline (PANI), an attractive conductive polymer, has been successfully applied in fabricating various types of enzyme-based biosensors. In this study, we have employed mesoporous silica SBA-15 to stably entrap horseradish peroxidase (HRP), and then deposited the loaded SBA-15 on the PANI modified platinum electrode to construct a GA/SBA-15(HRP)/PANI/Pt biosensor. The mesoporous structures and morphologies of SBA-15 with or without HRP were characterized. Enzymatic protein assays were employed to evaluate HRP immobilization efficiency. Our results demonstrated that the constructed biosensor displayed a fine linear correlation between cathodic response and H(2)O(2) concentration in the range of 0.02 to 18.5 mM, with enhanced sensitivity. In particular, the current approach provided the PANI modified biosensor with improved stability for multiple measurements.
authors.affiliation: Department of Chemical and Materials Engineering, Tunghai University, Taichung 40704, Taiwan; E-Mails:  g913125@thu.edu.tw  (C.C);  jsdo@thu.edu.tw  (J.D.).
authors.forename: Chien-Chung
authors.initials: CC
authors.lastname: Chen
authors.forename: Jing-Shan
authors.initials: JS
authors.lastname: Do
authors.forename: Yesong
authors.initials: Y
authors.lastname: Gu