title: Fluorescence enhancement of fluorescein isothiocyanate-labeled protein a caused by affinity binding with immunoglobulin g in bovine plasma.
abstract: Fluorescence enhancement of fluorescein isothiocyanate-labeled protein A (FITC-protein A) caused by the binding with immunoglobulin G (IgG) in bovine plasma was studied. FITC-protein A was immobilized onto a glass surface by covalent bonds. An increase in fluorescence intensity was dependent on IgG concentration ranging from 20 to 78 μg/mL in both phosphate buffer saline and bovine plasma. This method requires no separation procedure, and the reaction time is less than 15 min. A fluorescence enhancement assay by the affinity binding of fluorescence-labeled reagent is thus available for the rapid determination of biomolecules in plasma.
authors.affiliation: Department of Chemical Engineering, Waseda University / 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan; E-Mail:  t-ogawa@aoni.waseda.jp  (T.O.).
authors.forename: Takehito
authors.initials: T
authors.lastname: Ogawa
authors.forename: Satoka
authors.initials: S
authors.lastname: Aoyagi
authors.forename: Takehiro
authors.initials: T
authors.lastname: Miyasaka
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Sakai