title: Functional inclusion bodies produced in bacteria as naturally occurring nanopills for advanced cell therapies.
abstract: Inclusion bodies (50-500 nm in diameter) produced in recombinant bacteria can be engineered to contain functional proteins with therapeutic potential. Upon exposure, these protein particles are efficiently internalized by mammalian cells and promote recovery from diverse stresses. Being fully biocompatible, inclusion bodies are a novel platform, as tailored nanopills, for sustained drug release in advanced cell therapies.
authors.affiliation: Institut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, Bellaterra, Spain.
authors.forename: Esther
authors.initials: E
authors.lastname: Vázquez
authors.forename: José L
authors.initials: JL
authors.lastname: Corchero
authors.forename: Joan F
authors.initials: JF
authors.lastname: Burgueño
authors.forename: Joaquin
authors.initials: J
authors.lastname: Seras-Franzoso
authors.forename: Ana
authors.initials: A
authors.lastname: Kosoy
authors.forename: Ramon
authors.initials: R
authors.lastname: Bosser
authors.forename: Rosa
authors.initials: R
authors.lastname: Mendoza
authors.forename: Joan Marc
authors.initials: JM
authors.lastname: Martínez-Láinez
authors.forename: Ursula
authors.initials: U
authors.lastname: Rinas
authors.forename: Ester
authors.initials: E
authors.lastname: Fernández
authors.forename: Luis
authors.initials: L
authors.lastname: Ruiz-Avila
authors.forename: Elena
authors.initials: E
authors.lastname: García-Fruitós
authors.forename: Antonio
authors.initials: A
authors.lastname: Villaverde
mesh_terms: D000818:Animals; D002374:Catalase / Q000008:administration & dosage / Q000627:therapeutic use; D002460:Cell Line; D002463:Cell Membrane Permeability; D003692:Delayed-Action Preparations / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000008:administration & dosage; D018840:HSP70 Heat-Shock Proteins / Q000008:administration & dosage / Q000627:therapeutic use; D006367:HeLa Cells; D006801:Humans; D002479:Inclusion Bodies / Q000378:metabolism*; D053605:Leukemia Inhibitory Factor / Q000008:administration & dosage / Q000627:therapeutic use; D051379:Mice; D011994:Recombinant Proteins / Q000008:administration & dosage* / Q000627:therapeutic use; D013762:Tetrahydrofolate Dehydrogenase / Q000008:administration & dosage / Q000627:therapeutic use
chemical_list: D003692:Delayed-Action Preparations; D018840:HSP70 Heat-Shock Proteins; D053605:Leukemia Inhibitory Factor; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins; D002374:Catalase; D013762:Tetrahydrofolate Dehydrogenase