title: Bilateral common carotid artery ligation transiently changes brain lipid metabolism in rats.
abstract: Brain lipid metabolism was studied in rats following permanent bilateral common carotid artery ligation (BCCL), a model for chronic cerebral hypoperfusion. Unesterified (free) fatty acids (uFA) and acyl-CoA concentrations were measured 6 h, 24 h, and 7 days after BCCL or sham surgery, in high energy-microwaved brain. In BCCL compared to sham rats, cytosolic phospholipase A(2) (cPLA(2)) immunoreactivity in piriform cortex, and concentrations of total uFA and arachidonoyl-CoA, an intermediate for arachidonic acid reincorporation into phospholipids, were increased only at 6 h. At 24 h, immunoreactivity for secretory phospholipase A(2) (sPLA(2)), which may regulate blood flow, was increased near cortical and hippocampal blood vessels. BCCL did not affect levels of brain IB(4)+ microglia, glial fibrillary acidic protein (GFAP)+ astrocytes, cyclooxygenase-2 (COX-2) immunoreactivity at any time, but increased cPLA(2) immunoreactivity in one region at 6 h. Thus, BCCL affected brain lipid metabolism transiently, likely because of compensatory sPLA(2)-mediated vasodilation, without producing evidence of neuroinflammation.
authors.affiliation: Brain Physiology and Metabolism Section, National Institute on Aging, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD, USA.
authors.forename: Abesh Kumar
authors.initials: AK
authors.lastname: Bhattacharjee
authors.forename: Laura
authors.initials: L
authors.lastname: White
authors.forename: Lisa
authors.initials: L
authors.lastname: Chang
authors.forename: Kaizong
authors.initials: K
authors.lastname: Ma
authors.forename: G Jean
authors.initials: GJ
authors.lastname: Harry
authors.forename: Joseph
authors.initials: J
authors.lastname: Deutsch
authors.forename: Stanley I
authors.initials: SI
authors.lastname: Rapoport
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002339:Carotid Arteries / Q000378:metabolism / Q000473:pathology*; D002851:Chromatography, High Pressure Liquid; D005227:Fatty Acids / Q000378:metabolism; D007150:Immunohistochemistry; D050356:Lipid Metabolism; D008297:Male; D051381:Rats; D011921:Rats, Inbred WKY
chemical_list: D005227:Fatty Acids