title: Transcriptional response of the cellobiose dehydrogenase gene to cello- and xylooligosaccharides in the basidiomycete Phanerochaete chrysosporium.
abstract: Cellobiose dehydrogenase (CDH) gene transcripts were quantified by reverse transcription-PCR (RT-PCR) in cultures of Phanerochaete chrysosporium supplemented with various cello- and xylooligosaccharides in order to elucidate the mechanism of enhanced CDH production in xylan/cellulose culture. Cellotriose and cellotetraose induced cdh expression, while xylobiose and xylotriose induced expression of cellobiohydrolase genes, especially cel7C.
authors.affiliation: Department of Biomaterial Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
authors.forename: Chiaki
authors.initials: C
authors.lastname: Hori
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Suzuki
authors.forename: Kiyohiko
authors.initials: K
authors.lastname: Igarashi
authors.forename: Masahiro
authors.initials: M
authors.lastname: Samejima
mesh_terms: D002237:Carbohydrate Dehydrogenases / Q000096:biosynthesis*; D003470:Culture Media / Q000737:chemistry; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D009844:Oligosaccharides / Q000378:metabolism*; D020075:Phanerochaete / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D003470:Culture Media; D009844:Oligosaccharides; D002237:Carbohydrate Dehydrogenases; C019859:cellobiose-quinone oxidoreductase