title: Production of alkaline protease by Bacillus altitudinis GVC11 using castor husk in solid-state fermentation.
abstract: Castor (Ricinus communis L.) is an important oil seed crop having its main cultivated area in India, China, and Brazil in dry land farming. Castor husk is generated as waste in castor oil production. Use of castor husk waste as substrate is studied for alkaline protease production by Bacillus altitudinis GVC11 in solid-state fermentation. Various parameters like moisture content, incubation period, particle size, effect of carbon and nitrogen sources are studied and optimized for enzyme production. Highest enzyme production of 419,293 units per gram husk is obtained. Cost of enzyme production can be reduced by using castor husk as substrate.
authors.affiliation: Department of Microbiology, Osmania University, Hyderabad 500007, India.
authors.forename: A
authors.initials: A
authors.lastname: Madhuri
authors.forename: B
authors.initials: B
authors.lastname: Nagaraju
authors.forename: N
authors.initials: N
authors.lastname: Harikrishna
authors.forename: Gopal
authors.initials: G
authors.lastname: Reddy
mesh_terms: D001407:Bacillus / Q000378:metabolism*; D001426:Bacterial Proteins / Q000096:biosynthesis*; D001709:Biotechnology / Q000379:methods*; D002244:Carbon / Q000378:metabolism; D010450:Endopeptidases / Q000096:biosynthesis*; D005285:Fermentation; D007220:Industrial Waste; D009584:Nitrogen / Q000378:metabolism; D010316:Particle Size; D012278:Ricinus / Q000378:metabolism*; D013997:Time Factors
chemical_list: D001426:Bacterial Proteins; D007220:Industrial Waste; D002244:Carbon; D010450:Endopeptidases; C087822:alkaline protease; D009584:Nitrogen