title: Genetically determined heterogeneity of lung disease in a mouse model of airway mucus obstruction.
abstract: Mucus clearance is an important airway innate defense mechanism. Airway-targeted overexpression of the epithelial Na(+) channel β-subunit [encoded by sodium channel nonvoltage gated 1, beta subunit (Scnn1b)] in mice [Scnn1b-transgenic (Tg) mice] increases transepithelial Na(+) absorption and dehydrates the airway surface, which produces key features of human obstructive lung diseases, including mucus obstruction, inflammation, and air-space enlargement. Because the first Scnn1b-Tg mice were generated on a mixed background, the impact of genetic background on disease phenotype in Scnn1b-Tg mice is unknown. To explore this issue, congenic Scnn1b-Tg mice strains were generated on C57BL/6N, C3H/HeN, BALB/cJ, and FVB/NJ backgrounds. All strains exhibited a two- to threefold increase in tracheal epithelial Na(+) absorption, and all developed airway mucus obstruction, inflammation, and air-space enlargement. However, there were striking differences in neonatal survival, ranging from 5 to 80% (FVB/NJ<BALB/cJ<C3H/HeN<C57BL/6N), which correlated with the incidence of upper airway mucus plugging and the levels of Muc5b in bronchoalveolar lavage. The strains also exhibited variable Clara cell necrotic degeneration in neonatal intrapulmonary airways and a variable incidence of pulmonary hemorrhage and lung atelectasis. The spontaneous occurrence of a high surviving BALB/cJ line, which exhibited delayed onset of Na(+) hyperabsorption, provided evidence that: 1) air-space enlargement and postnatal death were only present when Na(+) hyperabsorption occurred early, and 2) inflammation and mucus obstruction developed whenever Na(+) hyperabsorption was expressed. In summary, the genetic context and timing of airway innate immune dysfunction critically determines lung disease phenotype. These mouse strains may be useful to identify key modifier genes and pathways.
authors.affiliation: Cystic Fibrosis/Pulmonary Research and Treatment Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA. alessandra_livraghi@med.unc.edu
authors.forename: Alessandra
authors.initials: A
authors.lastname: Livraghi-Butrico
authors.forename: Barbara R
authors.initials: BR
authors.lastname: Grubb
authors.forename: Elizabeth J
authors.initials: EJ
authors.lastname: Kelly
authors.forename: Kristen J
authors.initials: KJ
authors.lastname: Wilkinson
authors.forename: Huifang
authors.initials: H
authors.lastname: Yang
authors.forename: Marianne
authors.initials: M
authors.lastname: Geiser
authors.forename: Scott H
authors.initials: SH
authors.lastname: Randell
authors.forename: Richard C
authors.initials: RC
authors.lastname: Boucher
authors.forename: Wanda K
authors.initials: WK
authors.lastname: O'Neal
mesh_terms: D000402:Airway Obstruction / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D004195:Disease Models, Animal; D053503:Epithelial Sodium Channels / Q000235:genetics / Q000378:metabolism; D008173:Lung Diseases, Obstructive / Q000235:genetics*; D051379:Mice; D008815:Mice, Inbred Strains; D008822:Mice, Transgenic; D009093:Mucus / Q000378:metabolism*
chemical_list: D053503:Epithelial Sodium Channels; C507049:Scnn1b protein, mouse