title: The composition of the cuticular and internal free fatty acids and alcohols from Lucilia sericata males and females.
abstract: GC, GC-MS, and HPLC-LLSD analyses were used to identify and quantify cuticular and internal lipids in males and females of the blow-fly (Lucilia sericata). Sixteen free fatty acids, seven alcohols and cholesterol were identified and quantitatively determined in the cuticular lipids of L. sericata. Cuticular fatty acids ranged from C(6) to C(20) and included unsaturated entities such as 16:1n-9, 18:1n-9, 20:4n-3 and 20:5n-3. Cuticular alcohols (only saturated and even-numbered) ranged from C(12) to C(20) in males and C(10) to C(22) in females. Only one sterol was found in the cuticular lipids of both males and females. 23 free fatty acids, five alcohols and cholesterol were identified in the internal lipids. Internal fatty acids were present in large amounts-7.4 mg/g (female) and 10.1 mg/g (male). Only traces of internal alcohols (from C(14) to C(26) in males, from C(14) to C(22) in females) were found in L. sericata. Large amounts of internal cholesterol were identified in L. sericata males and females (0.49 and 0.97 mg/g of the insect body, respectively).
authors.affiliation: Institute for Environmental and Human Health Protection, Faculty of Chemistry, University of Gdańsk, ul. Sobieskiego 18/19, 80-952 Gdańsk, Poland. goleb@chem.univ.gda.pl
authors.forename: Marek
authors.initials: M
authors.lastname: Gołębiowski
authors.forename: Mieczysława I
authors.initials: MI
authors.lastname: Boguś
authors.forename: Monika
authors.initials: M
authors.lastname: Paszkiewicz
authors.forename: Wioletta
authors.initials: W
authors.lastname: Wieloch
authors.forename: Emilia
authors.initials: E
authors.lastname: Włóka
authors.forename: Piotr
authors.initials: P
authors.lastname: Stepnowski
mesh_terms: D000438:Alcohols / Q000737:chemistry / Q000302:isolation & purification*; D000818:Animals; D061046:Biological Control Agents; D002784:Cholesterol / Q000737:chemistry / Q000302:isolation & purification; D020092:Conidiobolus / Q000502:physiology; D004175:Diptera / Q000737:chemistry* / Q000382:microbiology; D060467:Disease Resistance; D005230:Fatty Acids, Nonesterified / Q000737:chemistry / Q000302:isolation & purification*; D005260:Female; D054884:Host-Pathogen Interactions; D008297:Male
chemical_list: D000438:Alcohols; D061046:Biological Control Agents; D005230:Fatty Acids, Nonesterified; D002784:Cholesterol