title: Isolation and characterization of multipotent postnatal stem/progenitor cells from human alveolar bone proper.
abstract: In the search for an ideal minimally-invasive multipotent postnatal stem cells' source, the aim of the present study was to isolate and characterize multipotent postnatal stem/progenitor cells from the human alveolar bone proper tissue of the oral cavity. Cells were isolated from human alveolar bone parts, immunomagnetically sorted using STRO-1 antibodies and characterized flow cytometrically for the expression of CD14, CD34, CD45, CD73, CD90, CD105, CD146/MUC18 and STRO-1 surface markers. Colony-formation and multilineage differentiation potential were tested. Mineralized tissue marker expression was examined using real time polymerase chain reaction (PCR). The cells were plastic-adherent and showed colony-formation. Cells expressed the surface markers CD73, CD90, CD105, STRO-1 and CD146/MUC18, while lacking the expression of the hematopoietic markers CD14, CD34 and CD45. Cells could be differentiated into osteoblastic, adipocytic and chondroblastic lineages. Unstimulated cells expressed alkaline phosphatase (ALP), type I, III and V collagens, osteonectin and osteocalcin in a very distinctive pattern. This study presents a practical and minimally-invasive scheme for the isolation of multipotent postnatal stem/progenitor cells from the human alveolar bone tissue of the oral cavity.
authors.affiliation: Clinic for Conservative Dentistry and Periodontology, School of Dental Medicine, Christian Albrechts University, Kiel, Germany. karim.fawzy@gmail.com
authors.forename: Karim M
authors.initials: KM
authors.lastname: Fawzy El-Sayed
authors.forename: Sebastian
authors.initials: S
authors.lastname: Paris
authors.forename: Stephan
authors.initials: S
authors.lastname: Becker
authors.forename: Neemat
authors.initials: N
authors.lastname: Kassem
authors.forename: Hendrick
authors.initials: H
authors.lastname: Ungefroren
authors.forename: Fred
authors.initials: F
authors.lastname: Fändrich
authors.forename: Jörg
authors.initials: J
authors.lastname: Wiltfang
authors.forename: Christof
authors.initials: C
authors.lastname: Dörfer
mesh_terms: D015720:5'-Nucleotidase / Q000032:analysis; D017667:Adipocytes / Q000166:cytology; D000469:Alkaline Phosphatase / Q000032:analysis; D000539:Alveolar Process / Q000166:cytology*; D015703:Antigens, CD / Q000032:analysis; D018952:Antigens, CD34 / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D051929:CD146 Antigen / Q000032:analysis; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods; D019902:Chondrocytes / Q000166:cytology; D024042:Collagen Type I / Q000032:analysis; D024061:Collagen Type III / Q000032:analysis; D024062:Collagen Type V / Q000032:analysis; D000071063:Endoglin; D005434:Flow Cytometry / Q000379:methods; D058851:GPI-Linked Proteins / Q000032:analysis; D006801:Humans; D017493:Leukocyte Common Antigens / Q000032:analysis; D018950:Lipopolysaccharide Receptors / Q000032:analysis; D008297:Male; D039902:Multipotent Stem Cells / Q000166:cytology*; D010006:Osteoblasts / Q000166:cytology; D015675:Osteocalcin / Q000032:analysis; D015676:Osteonectin / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction; D011956:Receptors, Cell Surface / Q000032:analysis; D018800:Thy-1 Antigens / Q000032:analysis
chemical_list: D015703:Antigens, CD; D018952:Antigens, CD34; D000954:Antigens, Surface; D051929:CD146 Antigen; D024042:Collagen Type I; D024061:Collagen Type III; D024062:Collagen Type V; C064206:ENG protein, human; D000071063:Endoglin; D058851:GPI-Linked Proteins; D018950:Lipopolysaccharide Receptors; C497902:MCAM protein, human; D015676:Osteonectin; D011956:Receptors, Cell Surface; C525591:STRO-1 antigen, human; D018800:Thy-1 Antigens; D015675:Osteocalcin; D000469:Alkaline Phosphatase; D017493:Leukocyte Common Antigens; C516769:PTPRC protein, human; D015720:5'-Nucleotidase; C548241:NT5E protein, human