title: Direct sensing of systemic and nutritional signals by haematopoietic progenitors in Drosophila.
abstract: The Drosophila lymph gland is a haematopoietic organ in which progenitor cells, which are most akin to the common myeloid progenitor in mammals, proliferate and differentiate into three types of mature cell--plasmatocytes, crystal cells and lamellocytes--the functions of which are reminiscent of mammalian myeloid cells. During the first and early second instars of larval development, the lymph gland contains only progenitors, whereas in the third instar, a medial region of the primary lobe of the lymph gland called the medullary zone contains these progenitors, and maturing blood cells are found juxtaposed in a peripheral region designated the cortical zone. A third group of cells referred to as the posterior signalling centre functions as a haematopoietic niche. Similarly to mammalian myeloid cells, Drosophila blood cells respond to multiple stresses including hypoxia, infection and oxidative stress. However, how systemic signals are sensed by myeloid progenitors to regulate cell-fate determination has not been well described. Here, we show that the haematopoietic progenitors of Drosophila are direct targets of systemic (insulin) and nutritional (essential amino acid) signals, and that these systemic signals maintain the progenitors by promoting Wingless (WNT in mammals) signalling. We expect that this study will promote investigation of such possible direct signal sensing mechanisms by mammalian myeloid progenitors.
authors.affiliation: Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, California 90095, USA.
authors.forename: Jiwon
authors.initials: J
authors.lastname: Shim
authors.forename: Tina
authors.initials: T
authors.lastname: Mukherjee
authors.forename: Utpal
authors.initials: U
authors.lastname: Banerjee
mesh_terms: D000601:Amino Acids, Essential / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D001773:Blood Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D002454:Cell Differentiation / Q000187:drug effects; D029721:Drosophila Proteins / Q000378:metabolism; D004331:Drosophila melanogaster / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D005508:Food Deprivation; D006410:Hematopoiesis / Q000187:drug effects; D007328:Insulin / Q000378:metabolism* / Q000494:pharmacology; D007814:Larva / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008221:Lymphoid Tissue / Q000166:cytology / Q000187:drug effects; D023461:Myeloid Progenitor Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D051155:Wnt1 Protein / Q000378:metabolism
chemical_list: D000601:Amino Acids, Essential; D029721:Drosophila Proteins; D007328:Insulin; D051155:Wnt1 Protein; C074622:wg protein, Drosophila