title: Nonplasmacytoid, high IFN-α-producing, bone marrow dendritic cells.
abstract: Plasmacytoid dendritic cells (pDC) are the producers of type I IFNs in response to TLR9 ligands. However, we have found that when bone marrow is depleted of pDC, the IFN-α produced in response to TLR9 ligands is not fully removed. We assign the source of this non-pDC IFN-α as a newly described DC type. It displays the high IFN-α producing activity of pDC but to a more limited range of viruses. Unlike pDC, the novel DC display high T cell stimulation capacity. Moreover, unlike mouse pDC, they are matured with GM-CSF and are less prone to apoptosis upon activation stimuli, including viruses. We propose that these DC constitute a novel bone marrow inflammatory DC type, ideally geared to linking innate and adaptive immune responses in bone marrow via their potent IFN-α production and high T cell stimulatory capacity.
authors.affiliation: Centre for Immunology, Burnet Institute, Melbourne, Victoria 3004, Australia. meredith@burnet.edu.au
authors.forename: Meredith
authors.initials: M
authors.lastname: O'Keeffe
authors.forename: Ben
authors.initials: B
authors.lastname: Fancke
authors.forename: Mark
authors.initials: M
authors.lastname: Suter
authors.forename: Georg
authors.initials: G
authors.lastname: Ramm
authors.forename: Joan
authors.initials: J
authors.lastname: Clark
authors.forename: Li
authors.initials: L
authors.lastname: Wu
authors.forename: Hubertus
authors.initials: H
authors.lastname: Hochrein
mesh_terms: D056704:Adaptive Immunity; D000818:Animals; D017209:Apoptosis; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology*; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000276:immunology / Q000494:pharmacology; D006801:Humans; D007113:Immunity, Innate; D016898:Interferon-alpha / Q000096:biosynthesis / Q000276:immunology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011070:Poly I-C / Q000494:pharmacology; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D051217:Toll-Like Receptor 9 / Q000235:genetics / Q000276:immunology; D014616:Vaccinia virus / Q000235:genetics / Q000276:immunology
chemical_list: D016898:Interferon-alpha; D008070:Lipopolysaccharides; C495370:Tlr9 protein, mouse; D051217:Toll-Like Receptor 9; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D011070:Poly I-C