title: The effects of nanofiber topography on astrocyte behavior and gene silencing efficiency.
abstract: Astrocyte-nanofiber interactions are studied by culturing primary rat cortical astrocytes on poly[caprolactone-co-(ethyl ethylene phosphate)] electrospun nanofibers and solvent-cast films (two-dimensional control). The results indicate that nanofiber topography significantly suppresses astrocyte proliferation and enhances apoptosis, without altering cellular activation as compared to films. Moreover, nanofiber topography enhances gene-silencing efficiency in astrocytes. The results suggest that nanofibers may serve as potential substrates for nerve regeneration by suppressing astrocyte growth and may further facilitate the use of gene-silencing to enhance CNS regeneration.
authors.affiliation: School of Chemical and Biomedical Engineering, Nanyang Technological University, 637459 Singapore.
authors.forename: Haoqing
authors.initials: H
authors.lastname: Cao
authors.forename: Guillaume
authors.initials: G
authors.lastname: Marcy
authors.forename: Eyleen Lay Keow
authors.initials: EL
authors.lastname: Goh
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Sing Yian
authors.initials: SY
authors.lastname: Chew
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D055664:Electrochemical Techniques; D004622:Embryo, Mammalian; D004705:Endocytosis / Q000187:drug effects; D020868:Gene Silencing; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D009416:Nerve Regeneration; D011091:Polyesters / Q000138:chemical synthesis* / Q000494:pharmacology; D061251:Primary Cell Culture; D034741:RNA, Small Interfering / Q000235:genetics; D051381:Rats; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D014162:Transfection
chemical_list: D011091:Polyesters; D034741:RNA, Small Interfering; C557288:poly(epsilon-caprolactone)-block-poly(ethyl ethylene phosphate)