title: Multidimensional plasma protein separation technique for identification of potential Alzheimer's disease plasma biomarkers: a pilot study.
abstract: Neurochemical differential diagnosis between Alzheimer's disease and other dementias is currently based on CSF biomarkers. Here, we report a method by which potential biomarkers can be identified in blood. Blood plasma samples from seven well-characterized Alzheimer's patients and seven non-Alzheimer's patients were subjected to a multi-dimensional protein separation procedure. After removal of 12 high-abundance proteins, the depleted samples from both diagnostic groups were labeled with different fluorescent dyes, mixed and separated by anion exchange and RP-chromatography. The resulting chromatography fractions were analyzed on 2D-gels. Twenty significant differentially expressed proteins were identified by mass spectrometry analysis. Ten of these proteins were either involved in the pathophysiology of Alzheimer's disease or the amyloid/Aβ-peptide processing pathway. This work demonstrated a successful application of a multidimensional separation technique and holds the potential to identifying blood-based biomarkers for other diseases in the future.
authors.affiliation: Department of Physiology, Faculty of Medicine, Jabriya, Kuwait University, P.O. Box 24923, Safat, 13110, Kuwait. andreas@hsc.edu.kw
authors.forename: Andreas Wolfram
authors.initials: AW
authors.lastname: Henkel
authors.forename: Katharina
authors.initials: K
authors.lastname: Müller
authors.forename: Piotr
authors.initials: P
authors.lastname: Lewczuk
authors.forename: Thorsten
authors.initials: T
authors.lastname: Müller
authors.forename: Katrin
authors.initials: K
authors.lastname: Marcus
authors.forename: Johannes
authors.initials: J
authors.lastname: Kornhuber
authors.forename: Jens
authors.initials: J
authors.lastname: Wiltfang
mesh_terms: D000368:Aged; D000544:Alzheimer Disease / Q000097:blood / Q000175:diagnosis*; D015415:Biomarkers / Q000097:blood*; D002845:Chromatography / Q000379:methods*; D003704:Dementia / Q000097:blood / Q000175:diagnosis*; D003937:Diagnosis, Differential; D006801:Humans; D010865:Pilot Projects; D040901:Proteomics / Q000379:methods*
chemical_list: D015415:Biomarkers