title: Solution NMR structure of the ribosomal protein RP-L35Ae from Pyrococcus furiosus.
abstract: The ribosome consists of small and large subunits each composed of dozens of proteins and RNA molecules. However, the functions of many of the individual protomers within the ribosome are still unknown. In this article, we describe the solution NMR structure of the ribosomal protein RP-L35Ae from the archaeon Pyrococcus furiosus. RP-L35Ae is buried within the large subunit of the ribosome and belongs to Pfam protein domain family PF01247, which is highly conserved in eukaryotes, present in a few archaeal genomes, but absent in bacteria. The protein adopts a six-stranded anti-parallel β-barrel analogous to the "tRNA binding motif" fold. The structure of the P. furiosus RP-L35Ae presented in this article constitutes the first structural representative from this protein domain family.
authors.affiliation: Department of Chemistry, College of Science and Health, William Paterson University, Wayne, New Jersey 07470, USA.
authors.forename: David A
authors.initials: DA
authors.lastname: Snyder
authors.forename: James M
authors.initials: JM
authors.lastname: Aramini
authors.forename: Bomina
authors.initials: B
authors.lastname: Yu
authors.forename: Yuanpeng J
authors.initials: YJ
authors.lastname: Huang
authors.forename: Rong
authors.initials: R
authors.lastname: Xiao
authors.forename: John R
authors.initials: JR
authors.lastname: Cort
authors.forename: Ritu
authors.initials: R
authors.lastname: Shastry
authors.forename: Li-Chung
authors.initials: LC
authors.lastname: Ma
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Liu
authors.forename: Burkhard
authors.initials: B
authors.lastname: Rost
authors.forename: Thomas B
authors.initials: TB
authors.lastname: Acton
authors.forename: Michael A
authors.initials: MA
authors.lastname: Kennedy
authors.forename: Gaetano T
authors.initials: GT
authors.lastname: Montelione
mesh_terms: D000595:Amino Acid Sequence; D019843:Archaeal Proteins / Q000737:chemistry* / Q000235:genetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D017434:Protein Structure, Tertiary; D020027:Pyrococcus furiosus / Q000737:chemistry* / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D012269:Ribosomal Proteins / Q000737:chemistry* / Q000235:genetics; D016415:Sequence Alignment; D055672:Static Electricity
chemical_list: D019843:Archaeal Proteins; D011994:Recombinant Proteins; D012269:Ribosomal Proteins