title: Development of a lateral flow immunoassay (LFA) strip for the rapid detection of 1-aminohydantoin in meat samples.
abstract: Due to the potential toxic effects of the nitrofuran family of antibiotics, their use in animals in the food industry has raised health concerns. This study was aimed to develop a lateral flow assay (LFA) based on competitive format for the detection of 1-aminohydantoin (AHD) in meat samples. The assay could be completed in 1 min and detected AHDs derivates (CPAHD) at 3 ng/mL, equivalent to 1.40 ng/mL of AHD, which was much lower than that reported in the literature by similar method. The antibody showed no cross-reactivity with a panel of more than 10 nitrofuran analogs except for nitrofurantoin at a high concentration. The test strip was stable at room temperature for up to 8 wk or at 37 °C for 4 wk. Parallel analyses of meat samples with LFA and enzyme-linked immunosorbent assay (ELISA) obtained data in good agreement. This developed gold nanoparticle based LFA had a good specificity, sensitivity, stability, and reliability. It was potentially suitable for on-the-spot large-scale screening of meat samples, and even more other applications.
  
  
  CONCLUSIONS
  Nitrofurantoin is one of antibiotics of the nitrofuran family, which has been used not only to prevent and treat diseases, but also to promote growth in animals. However, concerning the carcinogenicity of the metabolite of nitrofurantoin (AHD), a new fast and convenient method for monitoring AHD should be established. We describe the development of a new test assay for rapid screening of meat samples.
authors.affiliation: Molecular Immunology and Antibody Engineering Center, Jinan Univ., Guangzhou 510632, PR China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Tang
authors.forename: Xialing
authors.initials: X
authors.lastname: Xu
authors.forename: Xi
authors.initials: X
authors.lastname: Liu
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Huang
authors.forename: Yaoqiang
authors.initials: Y
authors.lastname: Chen
authors.forename: WuZhou
authors.initials: W
authors.lastname: Wang
authors.forename: Junjian
authors.initials: J
authors.lastname: Xiang
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000378:metabolism; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000378:metabolism; D002273:Carcinogens / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D003429:Cross Reactions; D004350:Drug Residues / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D005506:Food Contamination; D005513:Food Inspection / Q000379:methods*; D017979:Gold Colloid / Q000737:chemistry; D006827:Hydantoins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D006835:Hydrazones / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D007118:Immunoassay; D057230:Limit of Detection; D008460:Meat / Q000032:analysis*; D053768:Metal Nanoparticles / Q000737:chemistry; D009582:Nitrofurantoin / Q000032:analysis / Q000378:metabolism; D011934:Reagent Strips; D015203:Reproducibility of Results; D012710:Serum Albumin, Bovine / Q000737:chemistry; D034421:Sus scrofa; D013997:Time Factors
chemical_list: C504350:1-aminohydantoin; D000900:Anti-Bacterial Agents; D000911:Antibodies, Monoclonal; D002273:Carcinogens; D017979:Gold Colloid; D006827:Hydantoins; D006835:Hydrazones; D011934:Reagent Strips; D012710:Serum Albumin, Bovine; D009582:Nitrofurantoin