title: Isolation and characterization of hantaviruses in Far East Russia and etiology of hemorrhagic fever with renal syndrome in the region.
abstract: Hemorrhagic fever with renal syndrome (HFRS) is a serious public health issue in Far East Russia. Two different hantaviruses were isolated from rodents captured in the Khabarovsk region: Amur virus (AMRV; Khekhtsir/AP209/2005 strain from Apodemus peninsulae) and Hantaan virus (HTNV; Galkino/AA57/2002 strain from A. agrarius). Genetic analysis of the new isolates revealed that the M and L segments were apparently different between AMRV and HTNV, but S segments of the two viruses were closer. The antigenicities of AMRV, HTNV, and Seoul virus (SEOV) were differentiated by cross-neutralization. Serological differential diagnoses of 67 HFRS patients in the Prymorsky and Khabarovsk regions of Far East Russia were conducted using a neutralization test. The results revealed that the major cause of HFRS varied with location in Far East Russia: SEOV for Vladivostok city in the Prymorsky region, AMRV in rural areas of the Primorsky region, and probably HTNV for the Khabarovsk region.
authors.affiliation: Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan. kariwa@vetmed.hokudai.ac.jp
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kariwa
authors.forename: Keisuke
authors.initials: K
authors.lastname: Yoshikawa
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Tanikawa
authors.forename: Takahiro
authors.initials: T
authors.lastname: Seto
authors.forename: Takahiro
authors.initials: T
authors.lastname: Sanada
authors.forename: Ngonda
authors.initials: N
authors.lastname: Saasa
authors.forename: Leonid I
authors.initials: LI
authors.lastname: Ivanov
authors.forename: Raisa
authors.initials: R
authors.lastname: Slonova
authors.forename: Tatyana A
authors.initials: TA
authors.lastname: Zakharycheva
authors.forename: Ichiro
authors.initials: I
authors.lastname: Nakamura
authors.forename: Kumiko
authors.initials: K
authors.lastname: Yoshimatsu
authors.forename: Jiro
authors.initials: J
authors.lastname: Arikawa
authors.forename: Kentaro
authors.initials: K
authors.lastname: Yoshii
authors.forename: Ikuo
authors.initials: I
authors.lastname: Takashima
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D004279:DNA, Viral / Q000235:genetics*; D018063:Hantaan virus / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D006480:Hemorrhagic Fever with Renal Syndrome / Q000175:diagnosis / Q000503:physiopathology / Q000821:virology*; D006801:Humans; D051189:Murinae / Q000821:virology*; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D029263:Seoul virus / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D017422:Sequence Analysis, DNA
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral