title: Quantitative Proteomics of Intracellular Campylobacter jejuni Reveals Metabolic Reprogramming.
abstract: Campylobacter jejuni is the major cause of bacterial food-borne illness in the USA and Europe. An important virulence attribute of this bacterial pathogen is its ability to enter and survive within host cells. Here we show through a quantitative proteomic analysis that upon entry into host cells, C. jejuni undergoes a significant metabolic downshift. Furthermore, our results indicate that intracellular C. jejuni reprograms its respiration, favoring the respiration of fumarate. These results explain the poor ability of C. jejuni obtained from infected cells to grow under standard laboratory conditions and provide the bases for the development of novel anti microbial strategies that would target relevant metabolic pathways.
authors.affiliation: Section of Microbial Pathogenesis, Yale University School of Medicine, New Haven, Connecticut, United States of America.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Liu
authors.forename: Beile
authors.initials: B
authors.lastname: Gao
authors.forename: Veronica
authors.initials: V
authors.lastname: Novik
authors.forename: Jorge E
authors.initials: JE
authors.lastname: Galán
mesh_terms: D000818:Animals; D018407:Bacterial Physiological Phenomena; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D019556:COS Cells / Q000378:metabolism / Q000382:microbiology*; D016123:Campylobacter jejuni / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D065150:Cellular Reprogramming; D002522:Chlorocebus aethiops; D004734:Energy Metabolism; D005506:Food Contamination; D005650:Fumarates / Q000378:metabolism; D005786:Gene Expression Regulation; D054884:Host-Pathogen Interactions; D055442:Metabolome; D040901:Proteomics
chemical_list: D001426:Bacterial Proteins; D005650:Fumarates