title: [The development of method of detection of human pathogenic orthopoxviruses on the basis of polymerase chain reaction in real time].
abstract: The set of specific oligonucleotide primers and hybridization testers with the support of TaqMan in real time is proposed to identify and differentiate such human pathogenic orthopoxviruses as smallpox virus, monkey smallpox virus, cowpox virus, variolovaccine. The analytical specificity of the set is determined upon the example of 20 strains of 6 types of human pathogenic orthopoxviruses and made up 100%. The sensibility is established using recombinant plasmids containing orthopoxviruses sequence. The minimal revealed amount of plasmid made up to 20 copies for smallpox and monkey smallpox viruses, 10 copies for cowpox and 60 copies for variolovaccine in reaction.
authors.forename: D N
authors.initials: DN
authors.lastname: Shcherbakov
authors.forename: E V
authors.initials: EV
authors.lastname: Gavrilova
authors.forename: R A
authors.initials: RA
authors.lastname: Maksiutov
authors.forename: S N
authors.initials: SN
authors.lastname: Shelkunov
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015608:Cowpox virus / Q000302:isolation & purification; D017931:DNA Primers / Q000737:chemistry; D004279:DNA, Viral / Q000032:analysis* / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D018155:Orthopoxvirus / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011213:Poxviridae Infections / Q000175:diagnosis* / Q000821:virology; D012680:Sensitivity and Specificity; D014616:Vaccinia virus / Q000302:isolation & purification; D012901:Variola virus / Q000302:isolation & purification
chemical_list: D017931:DNA Primers; D004279:DNA, Viral