title: RGD-conjugated PLA-PLL nanoparticles targeting to Bacp-37 breast cancer xenografts in vivo.
abstract: Targeted delivery carriers are receiving considerable attention, the development of a more precise targeted delivery carrier is critical for the advancement of cancer chemotherapy. In this study, we evaluated the effects of RGD-conjugated poly (lactic acid-co-lysine)-(Arginine-Glycine-Aspartic) nanoparticles (PLA-PLL-RGD NPs) on targeted delivery to Bacp-37 breast cancer bearing mice. PLA-PLL-RGD NPs were prepared by using the emulsion-solvent evaporation method. A subsequent MTT assay indicated that the NPs were non-toxic and had good biocompatibility. In vitro, the results of Confocal Laser Scanning Microscope (CLSM) and FAC Scan flow cytometry (FACS) indicated that the PLA-PLL-RGD NPs can bind more significantly to human umbilical vein endothelial cells, compared to PLA-PLL NPs. In vivo, the results of target imaging and biodistribution showed that PLA-PLL-RGD can significantly target to tumor of Bacp-37 breast cancer bearing mice. These results demonstrated that PLA-PLL-RGD NPs can effectively enhance targeted efficiency in vivo, and have the potential to be used as targeted delivery carrier.
authors.affiliation: Shanghai Cancer Institute, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200032, PR China.
authors.forename: Peifeng
authors.initials: P
authors.lastname: Liu
authors.forename: Xuelian
authors.initials: X
authors.lastname: Qi
authors.forename: Ying
authors.initials: Y
authors.lastname: Sun
authors.forename: Hongzhi
authors.initials: H
authors.lastname: Wang
authors.forename: Yaogang
authors.initials: Y
authors.lastname: Li
authors.forename: Yourong
authors.initials: Y
authors.lastname: Duan
mesh_terms: D001672:Biocompatible Materials; D001943:Breast Neoplasms / Q000473:pathology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D008239:Lysine / Q000737:chemistry*; D018613:Microscopy, Confocal; D053758:Nanoparticles; D009842:Oligopeptides / Q000737:chemistry* / Q000493:pharmacokinetics; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D014018:Tissue Distribution; D023041:Xenograft Model Antitumor Assays
chemical_list: D001672:Biocompatible Materials; D009842:Oligopeptides; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); C047981:arginyl-glycyl-aspartic acid; D008239:Lysine