title: microRNA-133 inhibits cell proliferation, migration and invasion in             prostate cancer cells by targeting the epidermal growth factor receptor.
abstract: It has been shown that regulation of EGFR expression in prostate cancer             cells is mostly at the transcriptional level. microRNA-133 (miR-133) has long             been recognized as a muscle-specific miRNA which may regulate myoblast differentiation             and participate in many myogenic diseases. Recently, it has been reported that             miR-133 is also involved in other tumors, such as bladder cancer, esophageal cancer             and may regulate cell motility in these cancer cells. In the present study, we             examined the expression and effects of miR-133 in two hormone-insensitive prostate             cancer cell lines. The expression of miR-133a and miR-133b were analyzed by quantitative             RT-PCR. After transfection of miR-133a and miR-133b, cell viability assay, luciferase             assay, western blot analysis, cell migration and invasion assay were conducted             in Du145 and PC3 cells. In this study, we showed that miR‑133a and miR-133b are             expressed at the detection limit in two hormone-insensitive prostate cancer cell             lines, PC3 and DU145. Ectopic expression of miR-133 inhibited cell proliferation,             migration and invasion in these cells. We also provide the first evidence that             miR-133 may target EGFR. Our study provided the first glimpse of the functional             role of miR-133 in two hormone-independent prostate cancer cell lines. These results             may add to our knowledge on the molecular basis of prostate cancer progression.
authors.affiliation: Department of Urology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Tao
authors.forename: Deyao
authors.initials: D
authors.lastname: Wu
authors.forename: Bin
authors.initials: B
authors.lastname: Xu
authors.forename: Weichun
authors.initials: W
authors.lastname: Qian
authors.forename: Pengchao
authors.initials: P
authors.lastname: Li
authors.forename: Qiang
authors.initials: Q
authors.lastname: Lu
authors.forename: Changjun
authors.initials: C
authors.lastname: Yin
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D053487:DEAD-box RNA Helicases / Q000235:genetics; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D034622:RNA Interference; D034741:RNA, Small Interfering; D043244:Ribonuclease III / Q000235:genetics
chemical_list: C548234:MIRN133 microRNA, human; D035683:MicroRNAs; D034741:RNA, Small Interfering; D066246:ErbB Receptors; C408087:DICER1 protein, human; D043244:Ribonuclease III; D053487:DEAD-box RNA Helicases