title: Induction of protective immunity in chickens immunized with plant-made chimeric Bamboo mosaic virus particles expressing very virulent Infectious bursal disease virus antigen.
abstract: Very virulent Infectious bursal disease virus (vvIBDV) causes a highly contagious disease in young chickens and leads to significant economic loss in the poultry industry. Effective new vaccines are urgently needed. Autonomously replicating plant virus-based vector provides attractive means for producing chimeric virus particles (CVPs) in plants that can be developed into vaccines. In this study, we demonstrate the potential for vaccine development of Bamboo mosaic virus (BaMV) epitope-presentation system, where the antigen from vvIBDV VP2 was fused to the N-terminus of BaMV coat protein. Accordingly, an infections plasmid, pBIBD2, was constructed. Inoculation of the recombinant BaMV clone pBIBD2 enabled the generation of chimeric virus, BIBD2, and stable expression of IBDV VP2 antigen on its coat protein. After intramuscular immunization with BIBD2 CVPs, chickens produced antibodies against IBDV and were protected from vvIBDV (V263/TW strain) challenges. These results corroborate the feasibility of BaMV-based CVP platform in plants for the development and production of vaccines against IBDV.
authors.affiliation: Graduate Institute of Biotechnology, National Chung Hsing University, Taichung 402, Taiwan, ROC.
authors.forename: Tsung-Hsien
authors.initials: TH
authors.lastname: Chen
authors.forename: Ten-Hong
authors.initials: TH
authors.lastname: Chen
authors.forename: Chung-Chi
authors.initials: CC
authors.lastname: Hu
authors.forename: Jia-Teh
authors.initials: JT
authors.lastname: Liao
authors.forename: Chin-Wei
authors.initials: CW
authors.lastname: Lee
authors.forename: Jiunn-Wang
authors.initials: JW
authors.lastname: Liao
authors.forename: Maw-Yeong
authors.initials: MY
authors.lastname: Lin
authors.forename: Hung-Jen
authors.initials: HJ
authors.lastname: Liu
authors.forename: Min-Ying
authors.initials: MY
authors.lastname: Wang
authors.forename: Na-Sheng
authors.initials: NS
authors.lastname: Lin
authors.forename: Yau-Heiu
authors.initials: YH
authors.lastname: Hsu
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology*; D018175:Birnaviridae Infections / Q000276:immunology / Q000517:prevention & control; D002645:Chickens; D004195:Disease Models, Animal; D004337:Drug Carriers; D007243:Infectious bursal disease virus / Q000235:genetics / Q000276:immunology*; D007273:Injections, Intramuscular; D010944:Plants / Q000821:virology*; D010957:Plasmids; D017863:Potexvirus / Q000235:genetics* / Q000254:growth & development* / Q000302:isolation & purification; D011201:Poultry Diseases / Q000276:immunology / Q000517:prevention & control; D016019:Survival Analysis; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D004337:Drug Carriers; D014614:Vaccines, Synthetic; D014765:Viral Vaccines