title: Saliva and sera IgA and IgG in Egyptian Giardia-infected children.
abstract: Giardiasis is a gastrointestinal infection of wide distribution that is more prevalent in childhood. Easy and rapid diagnosis of giardiasis is essential for reduction of this infection. This cross-sectional study included 62 children in which collection of saliva, stool and serum samples was performed. An enzyme-linked immunosorbent assay (ELISA) technique was evaluated to detect IgA and IgG responses in both saliva and serum samples. Twenty-two children were positive for Giardia duodenalis infection by direct examination of faecal specimens, 20 non-infected and 20 infected with other parasites. Salivary and serum IgA and IgG responses against G. duodenalis infection were significantly higher in Giardia parasitized than non-Giardia parasitized children (p < 0.001). This concludes that specific salivary IgA may serve as a diagnostic tool and specific salivary IgG as a screening tool in monitoring the exposure of various populations to Giardia duodenalis. The advantage of salivary assays over serum immunoglobulin assay is being easy and non-invasive in sampling technique which is important especially for young children.
authors.affiliation: Department of Parasitology, Faculty of Medicine, Cairo University, Cairo, Egypt.
authors.forename: Naglaa Saad M
authors.initials: NS
authors.lastname: El-Gebaly
authors.forename: Eman Fawzy
authors.initials: EF
authors.lastname: Halawa
authors.forename: Hanaa M Ezzat
authors.initials: HM
authors.lastname: Moussa
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Rabia
authors.forename: Maha
authors.initials: M
authors.lastname: Abu-Zekry
mesh_terms: D002648:Child; D002675:Child, Preschool; D003430:Cross-Sectional Studies; D004534:Egypt / Q000453:epidemiology; D005260:Female; D005873:Giardiasis / Q000453:epidemiology / Q000276:immunology*; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood*; D007071:Immunoglobulin A, Secretory / Q000032:analysis*; D007074:Immunoglobulin G / Q000032:analysis / Q000097:blood*; D008297:Male; D012463:Saliva / Q000276:immunology*
chemical_list: D007070:Immunoglobulin A; D007071:Immunoglobulin A, Secretory; D007074:Immunoglobulin G