title: Antioxidant and hepatoprotective effects of the red ginseng essential oil in H(2)O(2)-treated hepG2 cells and CCl(4)-treated mice.
abstract: The aim of this study was to evaluate the antioxidant mechanisms of red ginseng essential oil (REO) in cells as well as in an animal model. REO was prepared by a supercritical CO(2) extraction of waste-products generated after hot water extraction of red ginseng. In HepG2 cells, REO diminished the H(2)O(2)-mediated oxidative stress and also restored both the activity and expression of antioxidant enzymes such as superoxide dismutase, catalase and glutathione peroxidase. Administration of REO inhibited the phosphorylation of upstream mitogen-activated protein kinases (MAPKs) such as c-Jun N-terminal kinase, extracellular signal-regulated kinase, and p38. In mice, the CCl(4)-mediated elevation of serum aspartate transaminase and alanine transaminase as well as the induction of hepatic lipid peroxidation were decreased by REO administration. REO treatments also resulted in up-regulation of the antioxidant enzyme expression in the liver. Moreover, increased phosphorylations of MAPKs were inhibited after REO administration. Overall, REO seems to protect the liver from oxidative stress through the activation and induction of antioxidant enzymes via inhibition of MAPKs pathways.
authors.affiliation: Institute for Phytochemical-Drug Interactions, Department of Food & Life Sciences, College of Biomedical Science & Engineering, Inje University, Gimhae 621-749, Korea.
authors.forename: Min-Ji
authors.initials: MJ
authors.lastname: Bak
authors.affiliation: Department of Food Science & Nutrition, Dong-A University, Busan 604-714, Korea.
authors.forename: Mira
authors.initials: M
authors.lastname: Jun
authors.affiliation: Institute for Phytochemical-Drug Interactions, Department of Food & Life Sciences, College of Biomedical Science & Engineering, Inje University, Gimhae 621-749, Korea.
authors.forename: Woo-Sik
authors.initials: WS
authors.lastname: Jeong
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002251:Carbon Tetrachloride / Q000633:toxicity*; D002252:Carbon Tetrachloride Poisoning / Q000517:prevention & control; D019610:Cytoprotection / Q000187:drug effects*; D056945:Hep G2 Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D008099:Liver / Q000187:drug effects*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009822:Oils, Volatile / Q000494:pharmacology*; D005894:Panax / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D009822:Oils, Volatile; D010936:Plant Extracts; D006861:Hydrogen Peroxide; D002251:Carbon Tetrachloride