title: Membrane-active metabolites produced by soil actinomycetes using chromatic phospholipid/polydiacetylene vesicles.
abstract: Increased resistance of pathogens toward existing antibiotics has compelled the research efforts to introduce new antimicrobial substances. Drugs with new and less resistant-prone targets to antimicrobial activity have a high priority for drug development activities. Cell membrane seems to be a potential target for new antibiotic agent development to overcome resistance. In this study, A total number of 67 actinomycetes were isolated from the soil samples collected from desert, farming and mineral parts of Iran. We used a chromatic sensor as a membrane model that was set up for the target of antimicrobial metabolites of actinomycetes isolated from the soil. The sensors particles were composed of phospholipid and polymerized polydiacetylene (PDA) lipids. These polymers exhibited color change following interaction with membrane-active metabolites. The color change was due to structural disorder in the lipids following their interaction with membrane-active metabolites. The resultant color change was recorded by fluorescent microscope and easily recognizable by naked eye as well. Sixteen strains were isolated which produced antimicrobial metabolites and were effective against test microorganisms (Escherichia coli, Candida albicans and Saccharomyces cerevisiae ). A total number of 3 out of 16 strains produced membrane-active metabolites. These 3 strains were identified using 16s rRNA as Streptomyces sp and submitted to GenBank (accession no. JN180853; JN180854; JN180855).
authors.affiliation: Drug Design and Bioinformatics Unit, Medical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran #69 Pasteur Ave.,Tehran, 13164, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Mehravar
authors.forename: Soroush
authors.initials: S
authors.lastname: Sardari
authors.forename: Parviz
authors.initials: P
authors.lastname: Owlia
mesh_terms: D039903:Actinobacteria / Q000302:isolation & purification / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology; D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology; D002176:Candida albicans / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects* / Q000648:ultrastructure; D003124:Colorimetry; D055808:Drug Discovery / Q000379:methods; D004926:Escherichia coli / Q000187:drug effects; D008567:Membranes, Artificial; D008826:Microbial Sensitivity Tests; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000737:chemistry*; D000078789:Polyacetylene Polymer; D011108:Polymers / Q000737:chemistry*; D053279:Polyynes / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000187:drug effects; D012988:Soil Microbiology; D012996:Solutions
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D000935:Antifungal Agents; D008567:Membranes, Artificial; D010743:Phospholipids; D000078789:Polyacetylene Polymer; D011108:Polymers; D012996:Solutions; D053279:Polyynes; C082361:polydiacetylene