title: The role of cytoskeleton, components of inositol phospholipid signaling pathway and iron in Ehrlichia canis in vitro proliferation.
abstract: Ehrlichia canis, etiologic agent of Canine Monocytic Ehrlichiosis, is an obligatory intracellular bacterium that parasitizes monocytes and macrophages. In this study we analyzed the role of the cytoskeleton specifically actin microfilaments and microtubules, components of inositol phospholipid signaling pathway such as phospholipase C (PLC), protein kinase (PTK) and calcium channels as well as the role of iron in the E. canis proliferation in DH82 cells. Different inhibitory compounds were used for each component: Cytochalasin D (inhibits actin polymerization), Nocodazole (inhibits microtubule polymerization), Neomycin (PLC inhibitor), Genistein (PTK inhibitor), Verapamil (calcium channel blocker) and Deferoxamine (iron chelator). We observed a significant decrease in the total number of bacteria in infected cells treated suggesting that these cellular components analized are essentials to E. canis proliferation.
authors.affiliation: Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, MG38400-902, Brazil. mal@icbim.ufu.br
authors.forename: Marcelo Arantes
authors.initials: MA
authors.lastname: Levenhagen
authors.forename: Rosiane Nascimento
authors.initials: RN
authors.lastname: Alves
authors.forename: Susana Elisa
authors.initials: SE
authors.lastname: Rieck
authors.forename: Marcelo Bahia
authors.initials: MB
authors.lastname: Labruna
authors.forename: Marcelo Emílio
authors.initials: ME
authors.lastname: Beletti
mesh_terms: D008841:Actin Cytoskeleton / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D015220:Calcium Channels / Q000378:metabolism; D002460:Cell Line; D004283:Dog Diseases / Q000378:metabolism / Q000382:microbiology*; D004285:Dogs; D041061:Ehrlichia canis / Q000254:growth & development*; D016873:Ehrlichiosis / Q000378:metabolism / Q000382:microbiology / Q000662:veterinary*; D007294:Inositol / Q000378:metabolism; D007501:Iron / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000662:veterinary; D008870:Microtubules / Q000187:drug effects* / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism; D015398:Signal Transduction; D010738:Type C Phospholipases / Q000378:metabolism
chemical_list: D015220:Calcium Channels; D007294:Inositol; D007501:Iron; D011494:Protein Kinases; D010738:Type C Phospholipases