title: Diagnostic potential of Fasciola gigantica-derived 14.5 kDa fatty acid binding protein in the immunodiagnosis of bubaline fascioliasis.
abstract: The 14.5 kDa fatty acid binding protein (FABP) was isolated from the crude extract of adult Fasciola gigantica worms. Polyclonal anti-FABP IgG was generated in rabbits immunized with prepared FABP antigen. Sandwich enzyme-linked immunosorbent assay (ELISA) was applied to detect coproantigen in stools and circulating Fasciola antigen (CA) in sera of 126 water buffaloes by using purified and horseradish peroxidase (HRP)-conjugated anti-FABP IgG. Sandwich ELISA sensitivity was 96.97% and 94.95%; while specificity was 94.12% and 82.35% for coproantigen and CA detection, respectively. However, sensitivity and specificity of the Kato-Katz technique was 73.74% and 100%, respectively. The diagnostic efficacy of sandwich ELISA was 96.55% and 93.1% for coproantigen and CA detection, respectively. In contrast, the diagnostic efficacy of the Kato-Katz technique was 77.59%. In conclusion, these results demonstrate that the purified 14.5 kDa FABP provides a more suitable antigen for immunodiagnosis of early and current bubaline fascioliasis by using sandwich ELISA.
authors.affiliation: Department of Zoology, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt. g.allam@tu.edu.sa
authors.forename: G
authors.initials: G
authors.lastname: Allam
authors.forename: I R
authors.initials: IR
authors.lastname: Bauomy
authors.forename: Z M
authors.initials: ZM
authors.lastname: Hemyeda
authors.forename: T M
authors.initials: TM
authors.lastname: Diab
authors.forename: T F
authors.initials: TF
authors.lastname: Sakran
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth; D000947:Antigens, Helminth / Q000032:analysis*; D001769:Blood / Q000469:parasitology; D002020:Buffaloes; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005211:Fascioliasis / Q000175:diagnosis / Q000662:veterinary*; D050556:Fatty Acid-Binding Proteins / Q000032:analysis*; D005243:Feces / Q000469:parasitology; D005260:Female; D007159:Immunologic Tests / Q000379:methods; D011817:Rabbits; D012680:Sensitivity and Specificity; D014730:Veterinary Medicine / Q000379:methods*
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D050556:Fatty Acid-Binding Proteins