title: A reporter system for replication-competent gammaretroviruses: the inGluc-MLV-DERSE assay.
abstract: Although novel retroviral vectors for use in gene-therapy products are reducing the potential for formation of replication-competent retrovirus (RCR), it remains crucial to screen products for RCR for both research and clinical purposes. For clinical-grade gammaretrovirus-based vectors, RCR screening is achieved by an extended S(+)L(-) or marker-rescue assay, whereas standard methods for replication-competent lentivirus detection are still in development. In this report, we describe a rapid and sensitive method for replication-competent gammaretrovirus detection. We used this assay to detect three members of the gammaretrovirus family and compared the sensitivity of our assay with well-established methods for retrovirus detection, including the extended S(+)L(-) assay. Results presented here demonstrate that this assay should be useful for gene-therapy product testing.
authors.affiliation: HIV Drug Resistance Program, National Cancer Institute, Frederick, MD 21702-1201, USA.
authors.forename: A L
authors.initials: AL
authors.lastname: Aloia
authors.forename: L
authors.initials: L
authors.lastname: Duffy
authors.forename: V
authors.initials: V
authors.lastname: Pak
authors.forename: K E
authors.initials: KE
authors.lastname: Lee
authors.forename: S
authors.initials: S
authors.lastname: Sanchez-Martinez
authors.forename: D
authors.initials: D
authors.lastname: Derse
authors.forename: G
authors.initials: G
authors.lastname: Heidecker
authors.forename: K
authors.initials: K
authors.lastname: Cornetta
authors.forename: A
authors.initials: A
authors.lastname: Rein
mesh_terms: D000818:Animals; D017930:Genes, Reporter / Q000235:genetics; D005822:Genetic Vectors; D057809:HEK293 Cells; D006801:Humans; D009052:Leukemia Virus, Murine / Q000235:genetics / Q000302:isolation & purification*; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D051379:Mice; D041681:NIH 3T3 Cells; D060888:Real-Time Polymerase Chain Reaction; D014779:Virus Replication
chemical_list: D008156:Luciferases