title: Functional and immunochemical cross-reactivity of V2-specific monoclonal antibodies from HIV-1-infected individuals.
abstract: The recent analysis of the first successful RV144 vaccine trial revealed that a high titer of plasma anti-V2 antibodies (Abs) correlated with a decreased risk of HIV-1 infection in vaccine recipients. To understand the mechanism of immune correlates, we studied seven anti-V2 monoclonal Abs (mAbs) developed from HIV-1 infected individuals. The V2 mAbs target conserved epitopes, including the binding site for α4β7 integrin, and are broadly cross-reactive with various gp120 proteins. Preferential usage of the VH1-69 gene by V2 mAbs may depend on selection by the same antigenic structure. Six of seven V2 mAbs weakly neutralized four to eight of the 41 pseudoviruses tested and resistance to neutralization was correlated with longer V2 domains. The data suggest the presence of shared, conserved structural elements in the V2 loop, and these can be used in the design of vaccine immunogens inducing broadly reactive Abs with anti-viral activities.
authors.affiliation: Department of Pathology, New York University School of Medicine, New York, NY 10016, USA. mirek.gorny@med.nyu.edu
authors.forename: Miroslaw K
authors.initials: MK
authors.lastname: Gorny
authors.forename: Ruimin
authors.initials: R
authors.lastname: Pan
authors.forename: Constance
authors.initials: C
authors.lastname: Williams
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Wang
authors.forename: Barbara
authors.initials: B
authors.lastname: Volsky
authors.forename: Timothy
authors.initials: T
authors.lastname: O'Neal
authors.forename: Brett
authors.initials: B
authors.lastname: Spurrier
authors.forename: Jared M
authors.initials: JM
authors.lastname: Sampson
authors.forename: Liuzhe
authors.initials: L
authors.lastname: Li
authors.forename: Michael S
authors.initials: MS
authors.lastname: Seaman
authors.forename: Xiang-Peng
authors.initials: XP
authors.lastname: Kong
authors.forename: Susan
authors.initials: S
authors.lastname: Zolla-Pazner
mesh_terms: D016915:AIDS Vaccines / Q000276:immunology*; D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000956:Antigens, Viral; D000939:Epitopes; D015483:HIV Antibodies; D015658:HIV Infections / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation
chemical_list: D016915:AIDS Vaccines; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D000939:Epitopes; D015483:HIV Antibodies