title: Low-dose steroid pretreatment ameliorates the transient impairment of liver regeneration.
abstract: OBJECTIVE
  To determine if liver regeneration (LR) could be disturbed following radiofrequency (RF) ablation and whether modification of LR by steroid administration occurs.
  
  
  METHODS
  Sham operation, partial hepatectomy (PH), and partial hepatectomy with radiofrequency ablation (PHA) were performed on adult Fisher 344 rats. We investigated the recovery of liver volume, DNA synthetic activities, serum cytokine/chemokine levels and signal transducers and activators of transcription 3 DNA-binding activities in the nucleus after the operations. Additionally, the effects of steroid (dexamethasone) pretreatment in the PH group (S-PH) and the PHA group (S-PHA) were compared.
  
  
  RESULTS
  The LR after PHA was impaired, with high serum cytokine/chemokine induction compared to PH, although the ratio of the residual liver weight to body weight was not significantly different. Steroid pretreatment disturbed LR in the S-PH group. On the other hand, low-dose steroid pretreatment improved LR and suppressed tumor necrosis factor (TNF)-α elevation in the S-PHA group, with recovery of STAT3 DNA-binding activity. On the other hand, low-dose steroid pretreatment improved LR and suppressed TNF-α elevation in the S-PHA group, with recovery of STAT3 DNA-binding activity.
  
  
  CONCLUSIONS
  LR is disturbed after RF ablation, with high serum cytokine/chemokine induction. Low-dose steroid administration can improve LR after RF ablation with TNF-α suppression.
authors.affiliation: Department of Surgery I, Sapporo Medical University Hospital, Sapporo, Hokkaido 060-8543, Japan.
authors.forename: Toshihito
authors.initials: T
authors.lastname: Shibata
authors.forename: Toru
authors.initials: T
authors.lastname: Mizuguchi
authors.forename: Yukio
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Masaki
authors.initials: M
authors.lastname: Kawamoto
authors.forename: Makoto
authors.initials: M
authors.lastname: Meguro
authors.forename: Shigenori
authors.initials: S
authors.lastname: Ota
authors.forename: Koichi
authors.initials: K
authors.lastname: Hirata
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Ooe
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Mitaka
mesh_terms: D000818:Animals; D017115:Catheter Ablation; D018925:Chemokines / Q000097:blood; D016207:Cytokines / Q000097:blood; D005260:Female; D006498:Hepatectomy / Q000379:methods*; D008099:Liver / Q000187:drug effects / Q000473:pathology / Q000502:physiology / Q000601:surgery; D008115:Liver Regeneration / Q000187:drug effects*; D051381:Rats; D011916:Rats, Inbred F344; D013256:Steroids / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D018925:Chemokines; D016207:Cytokines; D013256:Steroids; D014409:Tumor Necrosis Factor-alpha