title: A highly sensitive enzyme-amplified immunosensor based on a nanoporous niobium oxide (Nb2O5) electrode.
abstract: We report on the development of an enzyme-amplified sandwich-type immunosensor based on a thin gold film sputtered on an anodic nanoporous niobium oxide (Au@Nb(2)O(5)) electrode. The electrocatalytic activity of enzymatically amplified electroactive species and a stable electrode consisting of Au@Nb(2)O(5) were used to obtain a powerful signal amplification of the electrochemical immunobiosensor. The method using this electrochemical biosensor based on an Au@Nb(2)O(5) electrode provides a much better performance than those based on conventional bulk gold or niobium oxide electrodes. Our novel approach does not require any time-consuming cleaning steps to yield reproducible electrochemical signals. In addition, the strong adhesion of gold films on the niobium oxide electrodes offers a very stable substrate during electrochemical biosensing. Cyclic voltammetry measurements indicate that non-specific binding of proteins to the modified Au@Nb(2)O(5) surface is sufficiently low to be ignored in the case of our novel system. Finally, we demonstrated the ability of the biosensor based on an Au@Nb(2)O(5) offering the enhanced performance with a high resolution and sensitivity. Therefore, it is expected that the biosensor based on an Au@Nb(2)O(5) has great potential for highly efficient biological devices.
authors.affiliation: BioNanotechnology Research Center (BNRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-333, Korea. cslee@kribb.re.kr
authors.forename: Chang-Soo
authors.initials: CS
authors.lastname: Lee
authors.forename: Dohyoung
authors.initials: D
authors.lastname: Kwon
authors.forename: Jeng Eun
authors.initials: JE
authors.lastname: Yoo
authors.forename: Byung Gun
authors.initials: BG
authors.lastname: Lee
authors.forename: Jinsub
authors.initials: J
authors.lastname: Choi
authors.forename: Bong Hyun
authors.initials: BH
authors.lastname: Chung
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism*; D000814:Aniline Compounds / Q000378:metabolism; D000818:Animals; D002138:Calibration; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004566:Electrodes; D006046:Gold; D018625:Microscopy, Atomic Force; D058608:Nanopores; D009556:Niobium / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000378:metabolism; D010087:Oxides / Q000737:chemistry*; D011485:Protein Binding; D011487:Protein Conformation
chemical_list: D000814:Aniline Compounds; D009943:Organophosphorus Compounds; D010087:Oxides; D009556:Niobium; C068798:4-aminophenylphosphate; D006046:Gold; D000469:Alkaline Phosphatase; C073337:niobium pentoxide