title: Osteostatin-loaded onto mesoporous ceramics improves the early phase of bone regeneration in a rabbit osteopenia model.
abstract: Parathyroid hormone-related protein (PTHrP) is an important modulator of bone formation. Recently, we reported that PTHrP (107-111) (osteostatin) coating onto mesoporous ceramics confers osteogenic activity to these materials. Bone repair is dramatically compromised in osteopenia/osteoporosis. Thus, we examined the efficacy of unmodified and organically modified SBA15 ceramics loaded with osteostatin in promoting bone repair in an osteoporotic rabbit model. Osteoporosis was induced in New Zealand rabbits by methylprednisolone administration, and healthy rabbits were used as controls. Tested materials were implanted into a femoral cavitary defect, and animals were sacrificed at 2 weeks post-implantation. At this time, implants were encapsulated by a variable layer of fibrotic tissue with no evidence of inflammation. Similarly to observations in normal rabbits, both types of osteostatin-loaded bioceramics induced tissue regeneration associated with increased staining for PCNA, Runx2, osteopontin, and/or vascular endothelial growth factor in osteoporotic rabbits. Our present findings demonstrate that these osteostatin-bearing bioceramics increase the early repair response not only in normal bone but also in osteoporotic bone after a local injury.
authors.affiliation: Laboratorio de Metabolismo Mineral y Óseo, Instituto de Investigación Sanitaria (IIS)-Fundación Jiménez Díaz, Madrid, Spain.
authors.forename: Daniel
authors.initials: D
authors.lastname: Lozano
authors.forename: Cynthia G
authors.initials: CG
authors.lastname: Trejo
authors.forename: Enrique
authors.initials: E
authors.lastname: Gómez-Barrena
authors.forename: Miguel
authors.initials: M
authors.lastname: Manzano
authors.forename: Juan C
authors.initials: JC
authors.lastname: Doadrio
authors.forename: Antonio J
authors.initials: AJ
authors.lastname: Salinas
authors.forename: María
authors.initials: M
authors.lastname: Vallet-Regí
authors.forename: Natalio
authors.initials: N
authors.lastname: García-Honduvilla
authors.forename: Pedro
authors.initials: P
authors.lastname: Esbrit
authors.forename: Julia
authors.initials: J
authors.lastname: Buján
mesh_terms: D000818:Animals; D015519:Bone Density; D001851:Bone Diseases, Metabolic / Q000188:drug therapy* / Q000503:physiopathology; D001861:Bone Regeneration; D002516:Ceramics; D004195:Disease Models, Animal; D005260:Female; D007150:Immunohistochemistry; D044162:Parathyroid Hormone-Related Protein / Q000008:administration & dosage* / Q000627:therapeutic use; D010446:Peptide Fragments / Q000008:administration & dosage* / Q000627:therapeutic use; D011817:Rabbits
chemical_list: D044162:Parathyroid Hormone-Related Protein; D010446:Peptide Fragments; C071784:parathyroid hormone-related protein (107-111)