title: Evaluation of the ovarian reserve in women transplanted with frozen and thawed ovarian cortical tissue.
abstract: OBJECTIVE
  To investigate ovarian reserve and ovarian function in women transplanted with frozen/thawed ovarian tissue.
  
  
  METHODS
  Retrospective cohort study.
  
  
  METHODS
  University hospital.
  
  
  METHODS
  18 women transplanted with their own frozen/thawed ovarian tissue.
  
  
  METHODS
  None.
  
  
  METHODS
  Levels of antimüllerian hormone (AMH), duration of function of the transplanted ovarian tissue, outcome of assisted reproduction.
  
  
  RESULTS
  Of the 18 women who received transplanted ovarian tissue, levels of AMH were measured in 12 women; AMH never exceed a concentration of 1 ng/mL, and in several cases they were below the detection limit of the assay in combination with regular menstrual cycles. Two women with AMH below the detection limit conceived spontaneously. The duration of function of the transplants was between 9 months and 7 years and still functioning. Twelve women received assisted reproduction therapy; in 72 cycles, 65 oocytes were retrieved. The pregnancy rate and live-birth rate per cycle were 6.9% (5 of 72) and 2.8% (2 of 72), respectively.
  
  
  CONCLUSIONS
  The relatively poor outcome of assisted reproduction in women transplanted with frozen/thawed ovarian tissue may reflect reduced follicular selection rather than defective or aged oocytes. In normal women, reduced follicular selection with age may be part of explaining the decline in female fecundity with increasing age.
authors.affiliation: Laboratory of Reproductive Biology, Juliane Marie Centre for Women, Children and Reproduction, Copenhagen University Hospital, Copenhagen, Denmark.
authors.forename: Tine
authors.initials: T
authors.lastname: Greve
authors.forename: Kirsten Tryde
authors.initials: KT
authors.lastname: Schmidt
authors.forename: Stine Gry
authors.initials: SG
authors.lastname: Kristensen
authors.forename: Erik
authors.initials: E
authors.lastname: Ernst
authors.forename: Claus Yding
authors.initials: CY
authors.lastname: Andersen
mesh_terms: D000328:Adult; D054304:Anti-Mullerian Hormone / Q000097:blood; D015331:Cohort Studies; D015925:Cryopreservation; D005260:Female; D005298:Fertility; D005640:Follicle Stimulating Hormone / Q000097:blood; D006801:Humans; D006080:Ovarian Follicle / Q000637:transplantation*; D011247:Pregnancy; D018873:Pregnancy Rate; D027724:Reproductive Techniques, Assisted; D012189:Retrospective Studies; D020554:Sperm Injections, Intracytoplasmic; D014182:Transplantation, Autologous
chemical_list: D054304:Anti-Mullerian Hormone; D005640:Follicle Stimulating Hormone