title: Specific IgE measurement using AdvanSure® system: comparison of detection performance with ImmunoCAP® system in Korean allergy patients.
abstract: BACKGROUND
  AdvanSure enzyme immunoassay (EIA) system is a recently developed multiple allergen screen test with specific immunoglobulin E (sIgE) detection assay, while ImmunoCAP fluorescent EIA (FEIA) has been widely used for sIgE detection. There has been no inter-assay comparison data for these two systems. We determined the detection performance of AdvanSure system compared to that of ImmunoCAP.
  
  
  METHODS
  We performed an inter-method comparison using sera from 199 Korean allergy patients, including asthma (39.7%), allergic rhinitis (54.8%), atopic dermatitis (36.2%) and food allergies (21.6%). We compared the sIgE detection performance for nine major inhalant and four food allergens.
  
  
  RESULTS
  950 paired assay results were analyzed. Most allergen sIgE results showed above 0.5 intraclass correlation coefficient except Blattella germanica, alternaria and mugwort allergen. Intermethod comparison results showed multiple differences in a few allergens. The inter-method concordance was moderate to substantial for most allergens (κ=0.528-0.778, p<0.001), except for cat dander.
  
  
  CONCLUSIONS
  AdvanSure system showed a good detection performance compared with ImmunoCAP in correlation and agreement in Korean allergy patients. However, in terms of differences in the methodologies used by these two systems, careful clinical correlation is needed for interpretation of AdvanSure EIA results.
authors.affiliation: Division of Allergy and Immunology, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Republic of Korea.
authors.forename: Jae-Hyun
authors.initials: JH
authors.lastname: Lee
authors.forename: Kyung Hee
authors.initials: KH
authors.lastname: Park
authors.forename: Hyon-Suk
authors.initials: HS
authors.lastname: Kim
authors.forename: Kyung Won
authors.initials: KW
authors.lastname: Kim
authors.forename: Myung Hyun
authors.initials: MH
authors.lastname: Sohn
authors.forename: Chang-Hoon
authors.initials: CH
authors.lastname: Kim
authors.forename: Joo-Shil
authors.initials: JS
authors.lastname: Lee
authors.forename: Chein-Soo
authors.initials: CS
authors.lastname: Hong
authors.forename: Jung-Won
authors.initials: JW
authors.lastname: Park
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000485:Allergens / Q000097:blood / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D005260:Female; D005453:Fluorescence; D006801:Humans; D006967:Hypersensitivity / Q000097:blood* / Q000276:immunology*; D007124:Immunoenzyme Techniques / Q000379:methods / Q000592:standards*; D007073:Immunoglobulin E / Q000097:blood* / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D056910:Republic of Korea; D055815:Young Adult
chemical_list: D000485:Allergens; D007073:Immunoglobulin E