title: Clinicopathological and prognostic significance of microRNA-107 and             its relationship to DICER1 mRNA expression in gastric cancer.
abstract: microRNAs (miRNAs) are small non-coding RNAs that regulate target gene expression.             It is known that miRNA-107 (miR-107) promotes cancer invasion and metastasis.             However, the relationship between clinicopathological factors and the prognostic             significance of miR-107 for gastric cancer patients remains elusive. In this study,             we evaluated the prognostic value of miR-107 using tissue samples from gastric             cancer patients. Furthermore, the relationship between miR-107 and the mRNA levels             of its target gene DICER1 was examined. The expression levels of miR-107 and DICER1             mRNA in tumor tissues and adjacent normal tissues of 161 gastric cancer patients             were examined (TNM stage I, 29 patients; stage II, 31 patients; stage III, 51             patients and stage IV, 50 patients). miR-107 levels were measured by Taqman microRNA             assays, and DICER1 mRNA levels were measured by the Taqman real-time RT-PCR method.             In the analysis by real-time PCR-based miRNA arrays using pooled RNA samples from             five gastric cancer patients, expression of miR-107, miR-21, miR-196a, miR-26b,             miR-9, miR-142-3p, miR-30b, miR-150, miR-191 and miR-17 was found to be upregulation.             The mean expression level of miR-107 was significantly higher in the tumor tissues             compared to that of normal tissues. In the comparison of clinicopathological factors,             miR-107 expression showed significant association with depth of tumor invasion,             lymph node metastasis and stage. In Kaplan-Meier survival curve analysis, overall             survival rates (OS) and disease-free survival rates (DFS) of patients with high             miR-107 expression were significantly worse than those of patients with low miR-107             expression. In the Cox multivariate analysis, it was shown that miR-107 expression             in gastric cancer tissues was an independent prognostic factor for OS and DFS.             Significant inverse correlations were demonstrated between miR-107 and DICER1             mRNA. Our results indicate that miR-107 may be useful as an effective biomarker             for prediction of a poor prognosis in gastric cancer patients.
authors.affiliation: Department of Surgery, Teikyo University School of Medicine,                     Tokyo 173-0003, Japan.
authors.forename: Taisuke
authors.initials: T
authors.lastname: Inoue
authors.forename: Hisae
authors.initials: H
authors.lastname: Iinuma
authors.forename: Etsushi
authors.initials: E
authors.lastname: Ogawa
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Inaba
authors.forename: Ryoji
authors.initials: R
authors.lastname: Fukushima
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor; D053487:DEAD-box RNA Helicases / Q000235:genetics* / Q000378:metabolism; D018572:Disease-Free Survival; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D043244:Ribonuclease III / Q000235:genetics* / Q000378:metabolism; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000401:mortality* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; C549045:MIRN107 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; C408087:DICER1 protein, human; D043244:Ribonuclease III; D053487:DEAD-box RNA Helicases