title: Characterization of 10 tobacco vein banding mosaic virus isolates from China.
abstract: The complete RNA genome sequences of 10 tobacco vein banding mosaic virus (TVBMV) isolates from China were determined using five overlapping cDNA clones. TVBMV was divided into three groups for HC-Pro and CP and into two groups for P3 and 6K1. With more isolate sequences analyzed, the phylogenetic results suggest that TVBMV could be divided into four subgroups based on HC-Pro and CP and into three subgroups with P3 and 6K1. Nucleotide sequence diversity analysis showed geographical differentiation among the TVBMV isolates. Three of the 10 isolates were found to have undergone recombination and new recombination sites were identified in the TVBMV genome. All coding genes were under negative selection and the population appeared to have remained stable over a long period. This study also provides preliminary data on the 3´-untranslated region as potentially the best genome sequence for developing transgenic tobacco.
authors.affiliation: Shandong Agricultural University, Taian, People's Republic of China.
authors.forename: T
authors.initials: T
authors.lastname: Yuan
authors.forename: Y
authors.initials: Y
authors.lastname: Song
authors.forename: K
authors.initials: K
authors.lastname: Li
authors.forename: Q
authors.initials: Q
authors.lastname: Han
authors.forename: Ch
authors.initials: Ch
authors.lastname: Zhu
authors.forename: F
authors.initials: F
authors.lastname: Wen
mesh_terms: D020413:3' Untranslated Regions; D001483:Base Sequence; D002681:China; D003001:Cloning, Molecular; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D012367:RNA, Viral / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D011995:Recombination, Genetic; D012641:Selection, Genetic; D017422:Sequence Analysis, DNA; D014026:Nicotiana / Q000821:virology*; D014027:Tobacco Mosaic Virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D020413:3' Untranslated Regions; D012367:RNA, Viral