title: Effects of chronic kidney disease on blood cells membrane properties.
abstract: Chronic kidney disease (CKD) is progressive loss of renal function associated among others with increased intracellular calcium concentration. The purpose of this study was to identify the effects of CKD on cell membrane properties such as human red blood cell Ca(2+) ATPase activity, lymphocyte plasma membrane P2X(7) receptor expression and function. This could help us in elucidating the origin of increased calcium concentration in blood cells. We found out Ca(2+) ATPase activity is decreased in early stage CKD patients resulting in altered calcium removal from cytoplasm. By means of flow cytometry we assessed that P2X(7) receptor expression on lymphocyte membrane is 1.5 fold increased for CKD patients. Moreover, we detected an increased uptake of ethidium bromide through this receptor in CKD at basal conditions. It means CKD lymphocyte membranes contain more receptors which are more permeable thus allowing increased calcium influx from extracellular milieu. Finally, we can state alterations in blood cell membranes are closely linked to CKD and may be responsible for intracellular calcium accumulation.
authors.affiliation: Department of Nuclear Physics and Biophysics, Faculty of Mathematics, Physics and Informatics, Comenius University, Bratislava, Slovakia. zuzana.kaderjakova@gmail.com
authors.forename: Z
authors.initials: Z
authors.lastname: Kaderjakova
authors.forename: I
authors.initials: I
authors.lastname: Lajdova
authors.forename: M
authors.initials: M
authors.lastname: Horvathova
authors.forename: M
authors.initials: M
authors.lastname: Morvova
authors.forename: L
authors.initials: L
authors.lastname: Sikurova
mesh_terms: D001692:Biological Transport; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D016022:Case-Control Studies; D002462:Cell Membrane / Q000378:metabolism; D002463:Cell Membrane Permeability; D003593:Cytoplasm / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism* / Q000473:pathology; D004996:Ethidium / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D015870:Gene Expression; D006801:Humans; D007668:Kidney / Q000378:metabolism* / Q000473:pathology; D008214:Lymphocytes / Q000378:metabolism* / Q000473:pathology; D008297:Male; D058486:Receptors, Purinergic P2X7 / Q000235:genetics / Q000378:metabolism; D051436:Renal Insufficiency, Chronic / Q000378:metabolism* / Q000473:pathology
chemical_list: D058486:Receptors, Purinergic P2X7; D000252:Calcium-Transporting ATPases; D004996:Ethidium; D002118:Calcium