title: Complete mitochondrial genome of the red drum, Sciaenops ocellatus (Perciformes, Sciaenidae): absence of the typical conserved motif in the origin of the light-strand replication.
abstract: In this study, the complete mitochondrial genome of the red drum Sciaenops ocellatus was determined first. The genome was 16,500 bp in length and contained 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, and 2 main non-coding regions (the control region and the origin of the light-strand replication); the gene composition and order of which were similar to most other vertebrates. The overall base composition of the heavy strand was T 25.5%, C 30.7%, A 27.5%, and G 16.3%, with a slight AT bias of 53%. Within the control region, the discrete and conserved sequence blocks were identified. Motif 5'-ACCGG-3' rather than 5'-GCCGG-3' was detected in the origin of light-strand replication (O(L)) of red drum, which is rare in the mitogenomes of Sciaenidae species. These results would play an important role in elucidating sequence-function relationships of the O(L).
authors.affiliation: Key Laboratory for Marine Living Resources and Molecular Engineering, College of Marine Science, Zhejiang Ocean University, 316000 Zhoushan, People's Republic of China.
authors.forename: Yuanzhi
authors.initials: Y
authors.lastname: Cheng
authors.forename: Ge
authors.initials: G
authors.lastname: Shi
authors.forename: Tianjun
authors.initials: T
authors.lastname: Xu
authors.forename: Haiyan
authors.initials: H
authors.lastname: Li
authors.forename: Yueyan
authors.initials: Y
authors.lastname: Sun
authors.forename: Rixin
authors.initials: R
authors.lastname: Wang
mesh_terms: D000818:Animals; D001483:Base Sequence / Q000235:genetics*; D017124:Conserved Sequence / Q000235:genetics*; D004261:DNA Replication; D004272:DNA, Mitochondrial / Q000235:genetics; D023061:Gene Order; D050259:Genes, Mitochondrial; D054629:Genome, Mitochondrial / Q000235:genetics*; D010473:Perciformes / Q000145:classification / Q000235:genetics*; D018741:Replication Origin / Q000235:genetics*
chemical_list: D004272:DNA, Mitochondrial