title: Matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis of Gram-positive, catalase-negative cocci not belonging to the Streptococcus or Enterococcus genus and benefits of database extension.
abstract: Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry with a Bruker Daltonics microflex LT system was applied to 90 well-characterized catalase-negative, Gram-positive cocci not belonging to the streptococci or enterococci. Biotyper version 2.0.43.1 software was used singly or in combination with a database extension generated in this study with 51 collection strains from 16 genera. Most strains were identified by using both databases individually, and some were identified only by applying the combined database. Thus, the methodology is very useful and the generated database extension was helpful.
authors.affiliation: Department of Clinical Microbiology, Slagelse Hospital, Slagelse, Denmark. jejc@regionsjaelland.dk
authors.forename: Jens Jørgen
authors.initials: JJ
authors.lastname: Christensen
authors.forename: Rimtas
authors.initials: R
authors.lastname: Dargis
authors.forename: Monja
authors.initials: M
authors.lastname: Hammer
authors.forename: Ulrik Stenz
authors.initials: US
authors.lastname: Justesen
authors.forename: Xiaohui C
authors.initials: XC
authors.lastname: Nielsen
authors.forename: Michael
authors.initials: M
authors.lastname: Kemp
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D002374:Catalase / Q000378:metabolism; D006095:Gram-Positive Cocci / Q000737:chemistry* / Q000145:classification* / Q000201:enzymology; D012984:Software; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D002374:Catalase