title: Direct detection of Mycobacterium tuberculosis complex in clinical samples by a molecular method based on GenoQuick technology.
abstract: Several molecular systems for direct detection of Mycobacterium tuberculosis complex (MTBC) have recently been developed. The GenoQuick MTB assay (GQ-MTB) used in this study detected 82 of the 96 (85.4%) samples with MTBC, including 50 of 64 (78.1%) samples with negative acid-fast bacillus smears. Fifteen samples containing nontuberculous mycobacteria were also studied: 13 were GQ-MTB negative, one was positive, and one was indeterminate. GQ-MTB showed good effectiveness for the direct detection of MTBC from clinical samples.
authors.affiliation: Department of Microbiology, IDIBELL-Bellvitge Hospital, Feixa Llarga s/n, Hospitalet de Llobregat, Barcelona, Spain.
authors.forename: Raquel
authors.initials: R
authors.lastname: Moure
authors.forename: Miriam
authors.initials: M
authors.lastname: Torres
authors.forename: Rogelio
authors.initials: R
authors.lastname: Martín
authors.forename: Fernando
authors.initials: F
authors.lastname: Alcaide
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000175:diagnosis* / Q000382:microbiology*