title: [Effect of BYDV-MP nuclear localization signal on the movement of PVX].
abstract: Abstract:By using PVX derived vector pGR107, the effect of BYDV-MP nuclear localization signal on the movement of PVX was studied. BYDV-MP was cloned into pGR107 using GFP as an indicator. BYDV-MP was then shown to induce the systemic infection and exacerbate the symptom of PVX through infecting Nicotiana benthamiana. When the PVX gene encoding 25kD protein, which functioned as a systematic movemnet protein,was deleted and the above experiment was repeated, the result showed that BYDV-MP could compensate the systemic movement of PVX. A serial mutants with substitutions on the fifth, sixth and seventh amino acids of BYDV-MP nuclear localization signal was further constructed. It was found that the mutants at the fifth, sixth amino acids in BYDV-MP nuclear localization signal could only delay or weaken systemic movement of PVX whereas the mutant at seventh amino acid could entirely inhibit systemic movement of PVX.
authors.affiliation: College of Life Science, Liaocheng University, Liaocheng Shandong 252059, China.
authors.forename: Ji-Jun
authors.initials: JJ
authors.lastname: Yang
authors.forename: Guo-Fu
authors.initials: GF
authors.lastname: Liu
authors.forename: Yong-Mei
authors.initials: YM
authors.lastname: Shen
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Huo
authors.forename: Xue-Song
authors.initials: XS
authors.lastname: Cao
mesh_terms: D000595:Amino Acid Sequence; D049452:Green Fluorescent Proteins / Q000235:genetics; D017793:Luteovirus / Q000502:physiology*; D008969:Molecular Sequence Data; D019913:Nuclear Localization Signals / Q000737:chemistry / Q000502:physiology*; D053830:Plant Viral Movement Proteins / Q000502:physiology*; D017863:Potexvirus / Q000235:genetics / Q000502:physiology*
chemical_list: D019913:Nuclear Localization Signals; D053830:Plant Viral Movement Proteins; D049452:Green Fluorescent Proteins