title: Genetic analysis of a novel nidovirus from fathead minnows.
abstract: A bacilliform virus was isolated from diseased fathead minnows (Pimephales promelas). Analysis of the complete genome coding for the polyprotein (pp1ab), spike (S), membrane (M) and nucleocapsid (N) proteins revealed that the virus was most like white bream virus (WBV), another bacilliform virus isolated from white bream (Blicca bjoerkna L.) and the type species of the genus Bafinivirus within the order Nidovirales. In addition to similar gene order and size, alignment of deduced amino acid sequences of the pp1ab, M, N and S proteins of the fathead minnow nidovirus (FHMNV) with those of WBV showed 46, 44, 39 and 15 % identities, respectively. Phylogenetic analysis using the conserved helicase domain of the replicase showed FHMNV was distinct from WBV, yet the closest relative identified to date. Thus, FHMNV appears to represent a second species in the genus Bafinivirus. A PCR assay was developed for the identification of future FHMNV-like isolates.
authors.affiliation: US Geological Survey, Western Fisheries Research Center, 6505 Northeast 65th Street, Seattle, Washington 98115, USA.
authors.forename: William N
authors.initials: WN
authors.lastname: Batts
authors.affiliation: University of Arkansas at Pine Bluff, 1200 N. University Drive, Mail Slot 4912, Pine Bluff, Arkansas 71601, USA.
authors.forename: Andrew E
authors.initials: AE
authors.lastname: Goodwin
authors.affiliation: US Geological Survey, Western Fisheries Research Center, 6505 Northeast 65th Street, Seattle, Washington 98115, USA.
authors.forename: James R
authors.initials: JR
authors.lastname: Winton
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003530:Cyprinidae / Q000821:virology; D005393:Fish Diseases / Q000821:virology*; D014644:Genetic Variation; D008969:Molecular Sequence Data; D028381:Nidovirales / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D030341:Nidovirales Infections / Q000662:veterinary* / Q000821:virology; D010802:Phylogeny; D016415:Sequence Alignment; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D014764:Viral Proteins