title: Tamoxifen-loaded thiolated alginate-albumin nanoparticles as antitumoral drug delivery systems.
abstract: Nanoparticles based on disulfide bond reduced bovine serum albumin and thiolated alginate (alginate-cysteine conjugate) have been prepared by coacervation method and have been loaded with tamoxifen (TMX). The TMX load into the nanoparticles was optimized (4-6 μg/mg NP) by freeze-drying the systems before the loading procedure. Maximum TMX release (45-52%) took place between 2 and 25 h. Cytotoxicity of unloaded nanoparticles in MCF-7 and HeLa cells was not observed, although a small decrease in viability took place at very high concentration. Cell uptake of nanoparticles occurred in both cell types and the presence of polysaccharide in the nanoparticle composition allowed a better interaction with cells. The administration of 10 μM TMX by TMX-nanoparticles was effective in both cellular lines, and the effect of the drug-loaded systems on MCF-7 cell cycle showed the efficacy of the TMX-loaded nanoparticles.
authors.affiliation: Group of Polymeric Materials for the Controlled Release of Bioactive Compounds in Biomedicine, Departamento de Farmacología, Facultad de Farmacia, Universidad Complutense de Madrid, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Martínez
authors.forename: M
authors.initials: M
authors.lastname: Benito-Miguel
authors.forename: I
authors.initials: I
authors.lastname: Iglesias
authors.forename: J M
authors.initials: JM
authors.lastname: Teijón
authors.forename: M D
authors.initials: MD
authors.lastname: Blanco
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D018931:Antineoplastic Agents, Hormonal / Q000008:administration & dosage* / Q000494:pharmacology; D002417:Cattle; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006367:HeLa Cells; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D009369:Neoplasms / Q000188:drug therapy; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013629:Tamoxifen / Q000008:administration & dosage* / Q000494:pharmacology
chemical_list: D000464:Alginates; D018931:Antineoplastic Agents, Hormonal; D004337:Drug Carriers; D006603:Hexuronic Acids; D013438:Sulfhydryl Compounds; D013629:Tamoxifen; D012710:Serum Albumin, Bovine; D020723:Glucuronic Acid