title: Short-term creatine supplementation decreases reactive oxygen species content with no changes in expression and activity of antioxidant enzymes in skeletal muscle.
abstract: The effect of short-term creatine (Cr) supplementation upon content of skeletal muscle-derived-reactive oxygen species (ROS) was investigated. Wistar rats were supplemented with Cr (5 g/kg BW) or vehicle, by gavage, for 6 days. Soleus and extensor digitorum longus (EDL) muscles were removed and incubated for evaluation of ROS content using Amplex-UltraRed reagent. The analysis of expression and activity of antioxidant enzymes (superoxide dismutase 1 and 2, catalase and glutathione peroxidase) were performed. Direct scavenger action of Cr on superoxide radical and hydrogen peroxide was also investigated. Short-term Cr supplementation attenuated ROS content in both soleus and EDL muscles (by 41 and 33.7%, respectively). Cr supplementation did not change expression and activity of antioxidant enzymes. Basal TBARS content was not altered by Cr supplementation. In cell-free experiments, Cr showed a scavenger effect on superoxide radical in concentrations of 20 and 40 mM, but not on hydrogen peroxide. These results indicate that Cr supplementation decreases ROS content in skeletal muscle possibly due to a direct action of Cr molecule on superoxide radical.
authors.affiliation: Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, ICB-I, Cidade Universitária, Av. Prof. Lineu Prestes, 1524, Butantã, São Paulo, SP, 05508-900, Brazil. lucas@cefd.ufes.br
authors.forename: Lucas
authors.initials: L
authors.lastname: Guimarães-Ferreira
authors.forename: Carlos Hermano J
authors.initials: CH
authors.lastname: Pinheiro
authors.forename: Frederico
authors.initials: F
authors.lastname: Gerlinger-Romero
authors.forename: Kaio F
authors.initials: KF
authors.lastname: Vitzel
authors.forename: Renato T
authors.initials: RT
authors.lastname: Nachbar
authors.forename: Rui
authors.initials: R
authors.lastname: Curi
authors.forename: Maria Tereza
authors.initials: MT
authors.lastname: Nunes
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D003401:Creatine / Q000008:administration & dosage* / Q000494:pharmacology; D008297:Male; D018482:Muscle, Skeletal / Q000201:enzymology* / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D000072105:Superoxide Dismutase-1
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; C000606292:Sod1 protein, rat; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D003401:Creatine