title: Aqueous synthesis of Au:CdTe nanocrystals for noninvasive fluorescence imaging in living cells.
abstract: The gold-doped cadmium telluride (Au:CdTe) nanocrystals were synthesized by aqueous solution route using L-glutathione and L-cysteine as stabilizers. As-prepared Au:CdTe nanocrystals have good monodispersity and a zinc-blende structure. Compared with undoped CdTe nanocrystals, the Au:CdTe nanocrystals exhibited improved photostability, higher cellular affinity, and lower cytotoxicity. The Au:CdTe nanocrystals were used as probes for long-term noninvasive fluorescence imaging in living cells (The human lung epithelial carcinoma A549 cells). They could be endocytic uptaken by A549 cells and stably labeled the cytoplasm for over a week. By transmission electron microscopy (TEM) analysis, the Au:CdTe NCs could be observed in vesicles after being uptaken by A549 cells. Doping semiconductor nanocrystals with gold has the potential to engineer the photostability and biocompatibility for extensive biomedical applications. This work developed a facile aqueous solution route to synthesize gold-doped semiconductor nanocrystals and may assist in the design of doped nanobiomaterials.
authors.affiliation: Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Labaratory of Industrial Control Technology, Zhejiang University, Hangzhou 310058, China.
authors.forename: Sihai
authors.initials: S
authors.lastname: Han
authors.forename: Ying
authors.initials: Y
authors.lastname: Mu
authors.forename: Wei
authors.initials: W
authors.lastname: Jin
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Wu
authors.forename: Qiangyuan
authors.initials: Q
authors.lastname: Zhu
authors.forename: Qinhan
authors.initials: Q
authors.lastname: Jin
mesh_terms: D019187:Cadmium Compounds / Q000737:chemistry*; D002460:Cell Line; D006046:Gold / Q000737:chemistry*; D006801:Humans; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence / Q000379:methods*; D053758:Nanoparticles; D013056:Spectrophotometry, Ultraviolet; D013691:Tellurium / Q000737:chemistry*; D014867:Water
chemical_list: D019187:Cadmium Compounds; D014867:Water; D006046:Gold; D013691:Tellurium; C028337:cadmium telluride