title: Specific tumor-stroma interactions of EBV-positive Burkitt's lymphoma cells in the chick chorioallantoic membrane.
abstract: BACKGROUND
  Burkitt's lymphoma (BL) is an aggressive Non-Hodgkin lymphoma. Epstein-Barr Virus (EBV) is able to transform B cells and is a causative infectious agent in BL. The precise role of EBV in lymphoma progression is still unclear. Most investigations have concentrated on cell autonomous functions of EBV in B cells. Functions of the local environment in BL progression have rarely been studied, mainly due to the lack of appropriate in vivo models. Therefore, we inoculated different human BL cell-lines onto the chorioallantoic membrane (CAM) of embryonic day 10 (ED10) chick embryos and re-incubated until ED14 and ED17.
  
  
  RESULTS
  All cell-lines formed solid tumors. However, we observed strong differences in the behavior of EBV+ and EBV- cell-lines. Tumor borders of EBV+ cells were very fuzzy and numerous cells migrated into the CAM. In EBV- tumors, the borders were much better defined. In contrast to EBV- cells, the EBV+ cells induced massive immigration of chick leukocytes at the tumor borders and the development of granulation tissue with large numbers of blood vessels and lymphatics, although the expression of pro- and anti-angiogenic forms of Vascular Endothelial Growth Factors/receptors was the same in all BL cell-lines tested. The EBV+ cell-lines massively disseminated via the lymphatics and completely occluded them.
  
  
  CONCLUSIONS
  Our data suggest that the EBV+ cells attract pro-angiogenic leukocytes, which then induce secondary tumor-stroma interactions contributing to the progression of BL. We show that the CAM is a highly suitable in vivo model to study the differential behavior of BL cell-lines.
authors.affiliation: Department of Anatomy and Cell Biology, University Medicine Goettingen, 37075 Goettingen, Germany.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Becker
authors.affiliation: Department of Hematology and Oncology, University Medicine Goettingen, 37075 Goettingen, Germany.
authors.forename: Ana
authors.initials: A
authors.lastname: Covelo-Fernandez
authors.affiliation: Department of Hematology and Oncology, University Medicine Goettingen, 37075 Goettingen, Germany.
authors.forename: Frederike
authors.initials: F
authors.lastname: von Bonin
authors.affiliation: Department of Hematology and Oncology, University Medicine Goettingen, 37075 Goettingen, Germany.
authors.forename: Dieter
authors.initials: D
authors.lastname: Kube
authors.affiliation: Department of Anatomy and Cell Biology, University Medicine Goettingen, 37075 Goettingen, Germany.
authors.forename: Jörg
authors.initials: J
authors.lastname: Wilting