title: Adding denture cleanser to microwave disinfection regimen to reduce the irradiation time and the exposure of dentures to high temperatures.
abstract: BACKGROUND
  The microwave energy is an efficient disinfection method; however, it can generate high temperatures that can result in distortion of the dentures.
  
  
  OBJECTIVE
  To evaluate whether the addition of an enzymatic cleanser to microwave disinfection regimen would disinfect dentures with shorter irradiation time.
  
  
  METHODS
  Seven resin discs colonized with Candida albicans biofilm were placed on the palatal surface of sterile dentures to be randomly assigned to the following treatments: immersion in distilled water for 3 min with 0 (DW), 1 (DW + M1), 2 (DW + M2), or 3 min (DW + M3) of microwave irradiation; or immersion in denture cleanser for 3 min with 0 (DC), 1 (DC + M1), 2 (DC + M2) or 3 min (DC + M3) of irradiation. After the treatments, the viable cells were counted by a blinded examiner. The temperature was measured immediately after irradiation. The data were analyzed by ANOVA and Tukey post hoc tests (α = 0.05).
  
  
  RESULTS
  No viable cells were found after DC + M2, DC + M3, and DW + M3 treatments, of which DC + M2 achieved the lowest temperature. No significant difference was found between the effectiveness of DW, DW + M1 and DC treatments (p > 0.05).
  
  
  CONCLUSIONS
  Within the limits of this study, the association of a denture cleanser and microwave energy is efficient to disinfect dentures in lower irradiation time and temperature.
authors.affiliation: Department of Prosthodontics and Periodontology, Piracicaba Dental School, State University of Campinas, Piracicaba, São Paulo, Brazil.
authors.forename: Plinio M
authors.initials: PM
authors.lastname: Senna
authors.forename: Bruno S
authors.initials: BS
authors.lastname: Sotto-Maior
authors.forename: Wander J da
authors.initials: WJ
authors.lastname: Silva
authors.forename: Altair A
authors.initials: AA
authors.lastname: Del Bel Cury
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry / Q000528:radiation effects; D018441:Biofilms / Q000187:drug effects / Q000528:radiation effects; D002176:Candida albicans / Q000187:drug effects / Q000528:radiation effects; D015169:Colony Count, Microbial; D019551:Dental Disinfectants / Q000627:therapeutic use*; D003820:Denture Cleansers / Q000627:therapeutic use*; D003778:Dentures; D004203:Disinfection / Q000379:methods*; D006358:Hot Temperature; D006801:Humans; D008422:Materials Testing; D050296:Microbial Viability / Q000187:drug effects / Q000528:radiation effects; D008872:Microwaves / Q000627:therapeutic use*; D019904:Polymethyl Methacrylate / Q000737:chemistry / Q000528:radiation effects; D013499:Surface Properties; D013997:Time Factors; D014867:Water / Q000737:chemistry
chemical_list: D000180:Acrylic Resins; D019551:Dental Disinfectants; D003820:Denture Cleansers; D014867:Water; C040291:Lucitone; D019904:Polymethyl Methacrylate