title: Protective effects of fermented Citrus unshiu peel extract against ultraviolet-A-induced photoageing in human dermal fibrobolasts.
abstract: The aqueous extracts of Citrus unshiu peel containing flavonoid glycosides was used as co-substrate with Schizophyllum commune mycelia producing β-glucosidase and its biological activities were studied. β-glucosidase-produced S. commune mycelia converted the glycosides (narirutin and hesperidin) into aglycones (naringenin and hesperetin). The photoprotective potential of fermented C. unshiu peel extract with S. commune (S-CPE) was tested in human dermal fibroblasts (HDFs) exposed to UVA. It was revealed that S-CPE had an inhibitory effect on human interstitial collagenase (matrix metalloproteinase, MMP-1) expression in UVA-irradiated HDFs. The treatment of UVA-irradiated HDFs with S-CPE resulted in a dose-dependent decrease in the expression level of MMP-1 mRNA. The UVA irradiation raised the proportion of senescence-associated β-galactosidase (SA-β-gal) positive cells in comparison with the normal control group. The treatment of UVA-irradiated HDFs with S-CPE was shown to decrease the level of SA-β-gal (by approximately 45% at an S-CPE concentration 0.1%, w/v) compared with the UVA-irradiated HDFs. It was found that S-CPE containing hesperetin has notable collagen biosynthetic activity for fibroblasts, indicating that S-CPE can be promising cosmetic ingredients.
authors.affiliation: R & D Center, Hanbul Cosmetics Co., Chungbuk 369-830, Republic of Korea.
authors.forename: Jun Tae
authors.initials: JT
authors.lastname: Bae
authors.forename: Hyun Ju
authors.initials: HJ
authors.lastname: Ko
authors.forename: Gyoung Bum
authors.initials: GB
authors.lastname: Kim
authors.forename: Hyeong Bae
authors.initials: HB
authors.lastname: Pyo
authors.forename: Geun Soo
authors.initials: GS
authors.lastname: Lee
mesh_terms: D002478:Cells, Cultured; D002957:Citrus / Q000737:chemistry*; D003094:Collagen / Q000096:biosynthesis; D020405:Dermis / Q000166:cytology* / Q000528:radiation effects; D004187:Disaccharides / Q000378:metabolism; D005285:Fermentation; D005347:Fibroblasts / Q000187:drug effects* / Q000528:radiation effects*; D044950:Flavanones / Q000378:metabolism; D005638:Fruit / Q000737:chemistry; D006569:Hesperidin / Q000378:metabolism; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D012567:Schizophyllum / Q000254:growth & development / Q000378:metabolism; D015595:Skin Aging / Q000187:drug effects; D014466:Ultraviolet Rays; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D004187:Disaccharides; D044950:Flavanones; D010936:Plant Extracts; C500601:narirutin; D003094:Collagen; D006569:Hesperidin; D001616:beta-Galactosidase; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1; C005273:naringenin; C013015:hesperetin