title: Antioxidant activity and low toxicity of (E)-1-(1-(methylthio)-1-(selenopheny) hept-1-en-2-yl) pyrrolidin-2-one.
abstract: The aim of the present study was to evaluate the potential pharmacological and toxicological properties of (E)-1-(1-(methylthio)-1-(selenopheny) hept-1-en-2-yl) pyrrolidin-2-one (compound 1), an organoselenium compound. In vitro experiments showed that compound 1 presented a reduction in the lipid peroxidation induced by Fe²⁺ in thiobarbituric acid-reactive species (TBARS) production, and in the generation of reactive species caused by Fe²⁺/malonate in DCFH-DA oxidation. The high dose (500 mg/kg) induced an increase on ALT but not on AST activity. Hepatic, but not cerebral, δ-ALA-D activity from mice treated with 500 mg/kg presented a significant inhibition. Brain catalase activity was significantly inhibited by 100 mg/kg whereas hepatic catalase activity showed a significant increase at all doses. Hepatic lipid peroxidation was diminished only at lowest dose (100 mg/kg) whereas for brain tissue, all doses induced a significant reduction in TBARS levels. Brain and liver ascorbic acid contents were increased only at highest dose of compound 1. Urea and creatinine levels were not significantly altered by treatments. This is a promising compound with antioxidant activity and low toxicity, suggesting the potential beneficial activity of compound 1 against oxidative damage in many parameters studied in rats and mice.
authors.affiliation: Programa de Pós-Graduação em Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil.
authors.forename: Rafael Porto
authors.initials: RP
authors.lastname: Ineu
authors.forename: Matheus
authors.initials: M
authors.lastname: dos Santos
authors.forename: Olga
authors.initials: O
authors.lastname: Soares do Rêgo Barros
authors.forename: Cristina Wayne
authors.initials: CW
authors.lastname: Nogueira
authors.forename: João Batista Teixeira
authors.initials: JB
authors.lastname: Rocha
authors.forename: Gilson
authors.initials: G
authors.lastname: Zeni
authors.forename: Maria Ester
authors.initials: ME
authors.lastname: Pereira
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000704:Analysis of Variance; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology* / Q000633:toxicity; D001205:Ascorbic Acid / Q000378:metabolism; D001219:Aspartate Aminotransferases / Q000097:blood; D001921:Brain / Q000187:drug effects / Q000201:enzymology; D002374:Catalase / Q000378:metabolism; D003404:Creatinine / Q000097:blood; D005296:Ferrous Compounds / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D051379:Mice; D016566:Organoselenium Compounds / Q000494:pharmacology* / Q000633:toxicity; D016877:Oxidants / Q000494:pharmacology; D000623:Porphobilinogen Synthase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014020:Tissue Extracts; D014508:Urea / Q000097:blood
chemical_list: C575657:(E)-1-(1-(methylthio)-1-(selenophenyl)hept-1-en-2-yl)pyrrolidin-2-one; D000975:Antioxidants; D005296:Ferrous Compounds; D016566:Organoselenium Compounds; D016877:Oxidants; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; D014020:Tissue Extracts; D014508:Urea; D003404:Creatinine; D002374:Catalase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000623:Porphobilinogen Synthase; D001205:Ascorbic Acid