title: Single nucleotide polymorphisms analysis of noise-induced hearing loss using three-dimensional polyacrylamide gel-based microarray method.
abstract: Noise induced hearing loss (NIHL) is a complex occupational hazard caused by an interaction between genetic and environmental factors. Millions of Chinese industrial people are daily exposed to high level of noise. Although the environmental risk factors have been studied extensively, the nature of the genetic factors contributing to HIHL has not yet been clarified. In this study, we investigated 15 single nucleotide polymorphisms (SNPs) in 6 candidate genes influence susceptibility to noise in Chinese noise-exposed workers. Data from 3-dimensional polyacrylamide gel-based microarray platforms were analyzed. 103 blood samples were collected from noise-exposed laborers in Ningbo, Zhejiang, China. Subsequently, the interaction between noise exposure and genotypes and their effect on NIHL were analysed using logistic regression. Two interesting results were observed between noise exposure levels and genotypes of three SNPs, hence confirming that they are NIHL susceptibility genes in Chinese population.
authors.affiliation: Department of Otolaryngology, Beilun Branch of the First Affiliated Hospital (People's Hospital of Beilun), Medical College of Zhejiang University, Ningbo 315800, China.
authors.forename: Guihua
authors.initials: G
authors.lastname: Xia
authors.forename: Weiwei
authors.initials: W
authors.lastname: Gao
authors.forename: Ke
authors.initials: K
authors.lastname: Ji
authors.forename: Shaosheng
authors.initials: S
authors.lastname: Liu
authors.forename: Bo
authors.initials: B
authors.lastname: Wan
authors.forename: Junfeng
authors.initials: J
authors.lastname: Luo
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Bai
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D001483:Base Sequence; D002681:China; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006317:Hearing Loss, Noise-Induced / Q000235:genetics*; D006801:Humans; D016015:Logistic Models; D008969:Molecular Sequence Data; D009623:Noise, Occupational; D016273:Occupational Exposure; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods; D020641:Polymorphism, Single Nucleotide
chemical_list: D000180:Acrylic Resins; C016680:polyacrylamide gels