title: Serum antioxidant capacity and oxidative injury to pulmonary DNA in never-smokers with primary lung cancer.
abstract: OBJECTIVE
  Recently, in spite of the decrease in smoking in developed nations, the prevalence of primary lung cancer has been increasing in never-smokers. In the present study, we examined the status of oxidative stress and attempted to clarify the influence of oxidative stress in non-smoking patients with lung cancer.
  
  
  METHODS
  Sixty-one lung cancer patients who underwent a surgical resection, including 27 never-smokers and 34 ever-smokers with a history of more than 20 pack-years, were included. In addition, 18 surgical patients with benign lung diseases treated during the same period were also included as non-malignant controls. Using blood samples, both serum oxidative stress (OS) and anti-oxidant capacity (AOC) were examined with the derivatives of reactive oxygen metabolites (D-Roms) test and the biological antioxidant power (BAP) test, respectively. To assess the oxidative damage of the DNA in lung tissues, the non-lesion site tissues of the lung were immunohistochemically examined for the accumulation of thymidine glycol (TG).
  
  
  RESULTS
  There was no significant relationship between the serum OS level and various clinicopathological factors including the patient age, sex, body mass index, pathologic stage, and smoking status. On the other hand, the mean level of AOC was significantly lower in never-smokers than in ever-smokers. Although the mean TG-positive rate in ever-smokers was significantly higher than that in never-smokers, the mean TG-positive rate of the latter was significantly higher than that of patients with benign diseases.
  
  
  CONCLUSIONS
  The present study first demonstrated the low AOC in never-smokers with NSCLC, which may be a factor contributing to excessive oxidative DNA damage in the lung tissues.
authors.affiliation: Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan.
authors.forename: Kensaku
authors.initials: K
authors.lastname: Ito
authors.forename: Tokujiro
authors.initials: T
authors.lastname: Yano
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Morodomi
authors.forename: Tsukihisa
authors.initials: T
authors.lastname: Yoshida
authors.forename: Mikihiro
authors.initials: M
authors.lastname: Kohno
authors.forename: Akira
authors.initials: A
authors.lastname: Haro
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Shikada
authors.forename: Tatsuro
authors.initials: T
authors.lastname: Okamoto
authors.forename: Riichiroh
authors.initials: R
authors.lastname: Maruyama
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Maehara
mesh_terms: D000368:Aged; D000975:Antioxidants / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008168:Lung / Q000378:metabolism*; D008175:Lung Neoplasms / Q000097:blood*; D008297:Male; D018384:Oxidative Stress; D012907:Smoking
chemical_list: D000975:Antioxidants; D004247:DNA