title: Preparation of [In-111]-labeled-DTPA-bombesin conjugates at high specific activity and stability: evaluation of labeling parameters and potential stabilizers.
abstract: The aim of the present work was to obtain stabilized high specific activity (HSA) (111)In-labeled bombesin conjugates for preclinical evaluations. Parameters influencing the kinetics of labeling were investigated and the effect of stabilizers on HSA radiopeptides stability at room temperature were systematically categorized applying chromatography techniques. A SA of 174 GBq/μmol was achieved with high radiochemical purity, but the labeled compounds exhibited low stability. The addition of stabilizers avoided their radiolysis and significantly increased their stability.
authors.affiliation: Directory of Radiopharmacy, Nuclear and Energy Research Institute, Cidade Universitária da USP - Butantã, São Paulo, Brazil. pujatti.pb@gmail.com
authors.forename: P B
authors.initials: PB
authors.lastname: Pujatti
authors.forename: A V F
authors.initials: AV
authors.lastname: Massicano
authors.forename: J
authors.initials: J
authors.lastname: Mengatti
authors.forename: E B
authors.initials: EB
authors.lastname: de Araújo
mesh_terms: D001839:Bombesin / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D007205:Indium Radioisotopes / Q000737:chemistry*; D004369:Pentetic Acid / Q000737:chemistry*
chemical_list: D007205:Indium Radioisotopes; D004369:Pentetic Acid; D001839:Bombesin