title: Arabidopsis thaliana histone deacetylase 14 (HDA14) is an α-tubulin deacetylase that associates with PP2A and enriches in the microtubule fraction with the putative histone acetyltransferase ELP3.
abstract: It is now emerging that many proteins are regulated by a variety of covalent modifications. Using microcystin-affinity chromatography we have purified multiple protein phosphatases and their associated proteins from Arabidopsis thaliana. One major protein purified was the histone deacetylase HDA14. We demonstrate that HDA14 can deacetylate α-tubulin, associates with α/β-tubulin and is retained on GTP/taxol-stabilized microtubules, at least in part, by direct association with the PP2A-A2 subunit. Like HDA14, the putative histone acetyltransferase ELP3 was purified on microcystin-Sepharose and is also enriched at microtubules, potentially functioning in opposition to HDA14 as the α-tubulin acetylating enzyme. Consistent with the likelihood of it having many substrates throughout the cell, we demonstrate that HDA14, ELP3 and the PP2A A-subunits A1, A2 and A3 all reside in both the nucleus and cytosol of the cell. The association of a histone deacetylase with PP2A suggests a direct link between protein phosphorylation and acetylation.
authors.affiliation: Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, Alberta, Canada.
authors.forename: Hue T
authors.initials: HT
authors.lastname: Tran
authors.forename: Mhairi
authors.initials: M
authors.lastname: Nimick
authors.forename: R Glen
authors.initials: RG
authors.lastname: Uhrig
authors.forename: George
authors.initials: G
authors.lastname: Templeton
authors.forename: Nick
authors.initials: N
authors.lastname: Morrice
authors.forename: Robert
authors.initials: R
authors.lastname: Gourlay
authors.forename: Alison
authors.initials: A
authors.lastname: DeLong
authors.forename: Greg B G
authors.initials: GB
authors.lastname: Moorhead
mesh_terms: D000107:Acetylation; D017360:Arabidopsis / Q000166:cytology / Q000201:enzymology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D002467:Cell Nucleus / Q000201:enzymology; D003600:Cytosol / Q000201:enzymology; D051548:Histone Acetyltransferases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D006655:Histone Deacetylases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D052998:Microcystins / Q000737:chemistry; D008870:Microtubules / Q000201:enzymology*; D010766:Phosphorylation; D011485:Protein Binding; D025941:Protein Interaction Mapping; D054648:Protein Phosphatase 2 / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D011993:Recombinant Fusion Proteins; D014404:Tubulin / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D052998:Microcystins; D011993:Recombinant Fusion Proteins; D014404:Tubulin; C078588:microcystin; C502551:ELO3 protein, Arabidopsis; D051548:Histone Acetyltransferases; D054648:Protein Phosphatase 2; D006655:Histone Deacetylases; C000590683:histone deacetylase 14, Arabidopsis