title: Assessment of antibiofilm activity of magnesium fluoride nanoparticles-stabilized oil-in-water nanosized emulsion.
abstract: BACKGROUND
  Magnesium fluoride (MgF(2)) nanoparticles-stabilized oil-in-water nanosized emulsion was prepared and assessed for its antiadherent and antibiofilm activities over glass coupons against pathogenic microorganisms like Escherichia coli and Staphylococcus aureus.
  
  
  OBJECTIVE
  The major objectives of this paper are to synthesis MgF(2) nanoparticles, to prepare MgF(2) nanoparticles-stabilized nanosized emulsion, to coat glass coupons with MgF(2) nanoparticles and nanoparticles-stabilized emulsion, to challenge the emulsion or nanoparticles-coated and uncoated glass surfaces with fresh bacterial cultures over 18 h for biofilm formation, and to evaluate the extended antibiofilm activity of the coupons coated with either nanoparticles or emulsion in restricting the bacterial growth and biofilm formation.
  
  
  CONCLUSIONS
  Uncoated surfaces supported a massive biofilm formation (12.6 × 10(11) and 11.6 × 10(11) CFU cm(-2) for E. coli and S. aureus respectively for the 3rd day) while emulsion-coated surfaces dramatically restricted bacterial colonization (9.3 and 8.0 CFU cm(-2) for E. coli and S. aureus respectively). These results suggested that MgF(2) nanoparticles-stabilized emulsion is effective in restraining bacterial colonization on glass surfaces.
  
  
  CONCLUSIONS
  Although the glass coupons are selected as a model biomaterial surface, similar or increased antibiofilm action would be expected when this 'hybrid' nanoparticulate system is coated on other types of biomaterial surfaces such as intraocular lenses, catheters, etc.
authors.affiliation: Department of Pharmaceutical Technology , Bukit Jalil, Kuala Lumpur , Malaysia.
authors.forename: T
authors.initials: T
authors.lastname: Shunmugaperumal
authors.forename: S
authors.initials: S
authors.lastname: Ramamurthy