title: [The saliva cortisol level test using the automatic immunochemical analyzer Cobas e601 (Roche) to diagnose endogenous hypercorticalism in patients with obesity].
abstract: The saliva cortisol level test applies to diagnose endogenous hypercorticalism. However the methods of die format immunoassay traditionally used do not make it possible to get the study results on-the-fly. Also, reference interval and optimal takeoffs differ under implementing various techniques of cortisol tests. The purpose of actual study is to investigate the possibilities of electrochemiluminescent technique of testing free cortisol in saliva. The device Cobas e601 was applied to diagnose endogenous hypercorticalism in patients with obesity. The saliva samples were collected at 11 PM from 98 healthy volunteers and 123 patients with obesity (in 45 cases endogenous hypercorticalism was diagnosed). In total, 205 persons donated saliva at 11 PM two days running to evaluate the technique reproducibility. The samples of 197 individuals were frozen to implement the immune-enzyme assay. The minor test with dexamethasone was applied to patients with suspected endogenous hypercorticalism. The diagnosis of endogenous hypercorticalism was finally confirmed after the results of histological analysis of post-operative material or autopsy. Among healthy volunteers, the reference interval on indicators consisted 0.5-9.4 nMol/l. The correlation coefficient under free cortisol measuring at the same time two days running was -0.785. The optimal takeoff to diagnose endogenous hypercorticalism in patients with obesity consisted 9.4 nMol/l, sensitivity--84.4% (95% confidence band 71.2-92.2%), specificity--92.3% (95% confidence band 84.2-96.4%), predictive value of positive result--11.0 (95% confidence band 5.0-23.9), predictive value of negative result--0.17 (95% confidence band 0.08-0.33) and likelihood ratio for positive result--65.1 (95% confidence band 20.4-207.6). The two-fold cortisol test in saliva using immune-enzyme assay and minor test with dexamethasone with their diagnostic capabilities corresponded to one-fold saliva free cortisol test using electrochemiluminescent technique. The one-fold free cortisol test in saliva collected in 11 PM using the analyzer Cobas e601 for electrochemiluminescent immunoanalysis is a convenient and informative endogenous hypercorticalism screening technique in patients with obesity.
authors.forename: Zh E
authors.initials: ZhE
authors.lastname: Belaia
authors.forename: A V
authors.initials: AV
authors.lastname: Il'in
authors.forename: G A
authors.initials: GA
authors.lastname: Mel'nichenko
authors.forename: L Ia
authors.initials: LIa
authors.lastname: Rozhinskaia
authors.forename: N V
authors.initials: NV
authors.lastname: Dragunova
authors.forename: L K
authors.initials: LK
authors.lastname: Dzeranova
authors.forename: N A
authors.initials: NA
authors.lastname: Ogneva
authors.forename: S A
authors.initials: SA
authors.lastname: Butrova
authors.forename: E A
authors.initials: EA
authors.lastname: Troshina
authors.forename: G S
authors.initials: GS
authors.lastname: Kolesnikova
authors.forename: I I
authors.initials: II
authors.lastname: Dedov
mesh_terms: D000328:Adult; D015992:Body Mass Index; D003480:Cushing Syndrome / Q000150:complications / Q000175:diagnosis*; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D005260:Female; D006801:Humans; D006854:Hydrocortisone / Q000032:analysis* / Q000378:metabolism; D007124:Immunoenzyme Techniques / Q000379:methods; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000150:complications*; D012015:Reference Standards; D012463:Saliva / Q000737:chemistry*; D012680:Sensitivity and Specificity
chemical_list: D006854:Hydrocortisone