title: Determination of carisoprodol and meprobamate in oral fluid.
abstract: The determination of carisoprodol and its metabolite meprobamate in oral fluid using solid-phase extraction and liquid chromatography with tandem mass spectral detection (LC-MS-MS) and its application to authentic specimens is described. The method employs collection of oral fluid with the Quantisal device, extraction using cation exchange/hydrophobic solid-phase columns, and LC-MS-MS in positive ion electrospray mode. The method was fully validated using various parameters, including selectivity, linearity, accuracy, intra-day and inter-day imprecision, drug recovery from the collection pad, limit of quantitation and matrix effects. The method was applied to both routine research specimens and an authentic specimen taken from an individual prescribed a daily dose of 350 mg carisoprodol following surgery.
authors.affiliation: Immunalysis Corporation, 829 Towne Center Drive, Pomona, CA 91767, USA.
authors.forename: Cynthia
authors.initials: C
authors.lastname: Coulter
authors.forename: Margaux
authors.initials: M
authors.lastname: Garnier
authors.forename: James
authors.initials: J
authors.lastname: Tuyay
authors.forename: Jonathan
authors.initials: J
authors.lastname: Orbita
authors.forename: Christine
authors.initials: C
authors.lastname: Moore
mesh_terms: D002138:Calibration; D002328:Carisoprodol / Q000032:analysis* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008620:Meprobamate / Q000032:analysis*; D009125:Muscle Relaxants, Central / Q000032:analysis* / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D012463:Saliva / Q000737:chemistry*; D052616:Solid Phase Extraction; D021241:Spectrometry, Mass, Electrospray Ionization; D015813:Substance Abuse Detection; D053719:Tandem Mass Spectrometry
chemical_list: D009125:Muscle Relaxants, Central; D002328:Carisoprodol; D008620:Meprobamate