title: Phenotyping of circulating CD8⁺ T cell subsets in human cutaneous leishmaniasis.
abstract: Recovery from CL is usually accompanied with long-lasting protection and induction of strong immune response. The phenotypes, generation and maintenance of central (=T(CM)) and effector (=T(EM)) memory T cell subsets in human leishmaniasis are not well known. Profile of T cell subsets were analyzed on peripheral CD8⁺ T cells from volunteers with history of cutaneous leishmaniasis (HCL). In HCL and control groups, mean frequencies of CCR7⁺CD45RA⁺CD8⁺ naïve and CCR7⁻CD45RA⁻CD8⁺ T(EM) cells were higher than other subsets before culture, but after stimulation with soluble Leishmania antigen, the frequency of naïve T cells was significantly decreased and the frequency of T(EM) cells was significantly increased. T(EM) phenotype composed the highest portion of proliferating Carboxy Fluorescein diacetate Succinimidyl Ester (CFSE)-dim population which was significantly higher in HCL volunteers than in control group. Stimulation of isolated CD8⁺ memory T cells, but not naïve T cells, from HCL volunteers induced a significantly higher IFN-γ production compared with that of healthy controls. Intracellular IFN-γ staining provided the same result. Memory population is shown to be responsible for Leishmania-induced IFN-γ production. Leishmania-reactive proliferating T(EM) cells were identified as the most frequent subset which may play a role in recall immune response and protection against Leishmania infection.
authors.affiliation: Center for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, P.O. Box 14155-6383, Tehran, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Khamesipour
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Nateghi Rostami
authors.forename: Minoo
authors.initials: M
authors.lastname: Tasbihi
authors.forename: Akram
authors.initials: A
authors.lastname: Miramin Mohammadi
authors.forename: Tahereh
authors.initials: T
authors.lastname: Shahrestani
authors.forename: Abdolfattah
authors.initials: A
authors.lastname: Sarrafnejad
authors.forename: Yahya
authors.initials: Y
authors.lastname: Sohrabi
authors.forename: Seyed Ebrahim
authors.initials: SE
authors.lastname: Eskandari
authors.forename: Hossein
authors.initials: H
authors.lastname: Keshavarz Valian
mesh_terms: D000328:Adult; D018414:CD8-Positive T-Lymphocytes / Q000737:chemistry / Q000276:immunology*; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D007371:Interferon-gamma / Q000378:metabolism; D007891:Leishmania / Q000276:immunology; D016773:Leishmaniasis, Cutaneous / Q000276:immunology*; D017493:Leukocyte Common Antigens / Q000032:analysis; D008297:Male; D054400:Receptors, CCR7 / Q000032:analysis; D016176:T-Lymphocyte Subsets / Q000276:immunology*
chemical_list: C515370:CCR7 protein, human; D054400:Receptors, CCR7; D007371:Interferon-gamma; D017493:Leukocyte Common Antigens