title: Enzymatic production of xylooligosaccharides from alkali solubilized xylan of natural grass (Sehima nervosum).
abstract: In this study, a process for producing XOS from Sehima nervosum grass was developed. The grass contains 28.1% hemicellulose. NaOH and steam application yielded 98% of original xylan in contrast to 85% by KOH application. Hydrolysis of xylan with commercial xylanase caused breakdown into XOS comprising of xylobiose, xylotriose along with xylose. Response surface model (RSM) revealed highest xylobiose yield (11 g/100g xylan) at pH 5.03, temperature 45.19°C, reaction time 10.11h with enzyme dose 17.41 U. Similarly for maximizing xylotriose yield, ideal hydrolysis conditions were pH 5.11, temperature 40.33°C, reaction time 16.55 h with enzyme dose 13.20 U. A two step process encompassing xylan fractionation and enzymatic hydrolysis enabled XOS production from the S. nervosum grass.
authors.affiliation: National Institute of Animal Nutrition and Physiology, Bangalore 560030, India. drashiskumarsamanta@gmail.com
authors.forename: A K
authors.initials: AK
authors.lastname: Samanta
authors.forename: Natasha
authors.initials: N
authors.lastname: Jayapal
authors.forename: A P
authors.initials: AP
authors.lastname: Kolte
authors.forename: S
authors.initials: S
authors.lastname: Senani
authors.forename: Manpal
authors.initials: M
authors.lastname: Sridhar
authors.forename: K P
authors.initials: KP
authors.lastname: Suresh
authors.forename: K T
authors.initials: KT
authors.lastname: Sampath
mesh_terms: D000468:Alkalies / Q000494:pharmacology*; D004187:Disaccharides / Q000378:metabolism; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism*; D005965:Glucuronates / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D006868:Hydrolysis / Q000187:drug effects; D006878:Hydroxides / Q000494:pharmacology; D009844:Oligosaccharides / Q000096:biosynthesis*; D006109:Poaceae / Q000737:chemistry*; D017680:Potassium Compounds / Q000494:pharmacology; D012972:Sodium Hydroxide / Q000494:pharmacology; D012995:Solubility / Q000187:drug effects; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D013997:Time Factors; D014312:Trisaccharides / Q000378:metabolism; D014990:Xylans / Q000302:isolation & purification / Q000378:metabolism*; D014994:Xylose / Q000378:metabolism
chemical_list: D000468:Alkalies; D004187:Disaccharides; D005965:Glucuronates; D006878:Hydroxides; D009844:Oligosaccharides; D017680:Potassium Compounds; D014312:Trisaccharides; D014990:Xylans; C570991:xylooligosaccharide; C515044:xylotriose; D012972:Sodium Hydroxide; D014994:Xylose; D043364:Endo-1,4-beta Xylanases; C004173:xylobiose; C029943:potassium hydroxide