title: Evaluation of uptake and transport of cationic and anionic ultrasmall iron oxide nanoparticles by human colon cells.
abstract: Nanoparticles (NPs) are in clinical use or under development for therapeutic imaging and drug delivery. However, relatively little information exists concerning the uptake and transport of NPs across human colon cell layers, or their potential to invade three-dimensional models of human colon cells that better mimic the tissue structures of normal and tumoral colon. In order to gain such information, the interactions of biocompatible ultrasmall superparamagnetic iron oxide nanoparticles (USPIO NPs) (iron oxide core 9-10 nm) coated with either cationic polyvinylamine (aminoPVA) or anionic oleic acid with human HT-29 and Caco-2 colon cells was determined. The uptake of the cationic USPIO NPs was much higher than the uptake of the anionic USPIO NPs. The intracellular localization of aminoPVA USPIO NPs was confirmed in HT-29 cells by transmission electron microscopy that detected the iron oxide core. AminoPVA USPIO NPs invaded three-dimensional spheroids of both HT-29 and Caco-2 cells, whereas oleic acid-coated USPIO NPs could only invade Caco-2 spheroids. Neither cationic aminoPVA USPIO NPs nor anionic oleic acid-coated USPIO NPs were transported at detectable levels across the tight CacoReady™ intestinal barrier model or the more permeable mucus-secreting CacoGoblet™ model.
authors.affiliation: Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland.
authors.forename: Blanka Halamoda
authors.initials: BH
authors.lastname: Kenzaoui
authors.forename: Maya R
authors.initials: MR
authors.lastname: Vilà
authors.forename: Josep M
authors.initials: JM
authors.lastname: Miquel
authors.forename: Feride
authors.initials: F
authors.lastname: Cengelli
authors.forename: Lucienne
authors.initials: L
authors.lastname: Juillerat-Jeanneret
mesh_terms: D000838:Anions / Q000737:chemistry / Q000493:pharmacokinetics; D018938:Caco-2 Cells; D002412:Cations / Q000737:chemistry / Q000493:pharmacokinetics; D002470:Cell Survival / Q000187:drug effects; D003106:Colon / Q000166:cytology / Q000378:metabolism*; D019073:HT29 Cells; D006651:Histocytochemistry; D006801:Humans; D042541:Intracellular Space; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D019301:Oleic Acid / Q000737:chemistry / Q000493:pharmacokinetics; D010316:Particle Size; D011145:Polyvinyls / Q000737:chemistry; D018874:Spheroids, Cellular / Q000378:metabolism
chemical_list: D000838:Anions; D002412:Cations; D058185:Magnetite Nanoparticles; D011145:Polyvinyls; C534904:polyvinylamine; D019301:Oleic Acid