title: Association between two polymorphisms in the HLA-G gene and angiographic             coronary artery disease.
abstract: Atherosclerosis and related complications still represent the major cause of morbidity and mortality in industrialized countries. Therefore, it is particularly important to investigate the molecules involved in cardiac inflammation. Evidence exists showing that the human leukocyte antigen‑G (HLA-G) gene tissue expression and related protein physiological significance is influenced by two polymorphisms, rs16375 and rs1632933. In this study, allelic, genotypic and haplotypic frequencies of a 14-bp insertion/deletion (Ins/Del) (rs16375) and of rs1632933 polymorphisms of the HLA-G gene were investigated in 664 patients with coronary artery disease (CAD) and 345 matched controls by polymerase chain reaction (PCR)-restriction fragment length polymorphism analysis and real-time PCR. The frequency of the Ins/Ins genotype was significantly higher in patients with CAD compared to the controls (P=0.018). After analysis of confounding variables, the results showed that the homozygous Ins/Ins was significantly and independently associated with the presence of angiographic CAD (odds ratio 2.09, 95% confidence interval 1.10-4.02, P=0.03). Our data demonstrate a new risk factor for this multifactorial inflammatory disease.
authors.affiliation: Laboratory of Immunogenetics, Department of Genetics and Microbiology, University of Pavia, Pavia, Italy. chiara.boiocchi@unipv.it
authors.forename: Chiara
authors.initials: C
authors.lastname: Boiocchi
authors.forename: Sara
authors.initials: S
authors.lastname: Bozzini
authors.forename: Michele
authors.initials: M
authors.lastname: Zorzetto
authors.forename: Gabriele
authors.initials: G
authors.lastname: Pelissero
authors.forename: Mariaclara
authors.initials: M
authors.lastname: Cuccia
authors.forename: Colomba
authors.initials: C
authors.lastname: Falcone
mesh_terms: D000368:Aged; D017023:Coronary Angiography / Q000379:methods; D003324:Coronary Artery Disease / Q000000981:diagnostic imaging / Q000235:genetics*; D005260:Female; D059951:HLA-G Antigens / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D059951:HLA-G Antigens