title: Bulk- and surface-modified combinable PDMS capillary sensor array as an easy-to-use sensing device with enhanced sensitivity to elevated concentrations of multiple serum sample components.
abstract: To enhance sensitivity and facilitate easy sample introduction into a combinable poly(dimethylsiloxane) (PDMS) capillary (CPC) sensor array, PDMS was modified in bulk and on its surface to prepare "black" PDMS coated with a silver layer and self-assembled monolayer (SAM). India ink, a traditional Japanese black ink, was added to the PDMS pre-polymer for bulk modification. The surface was modified by a silver mirror reaction followed by SAM formation using cysteine. These modifications enhanced the fluorescence signals by reflecting them from the surface and reducing background interference. A decrease in the water contact angle led to enhanced sensitivity and easy sample introduction. Furthermore, a CPC sensor array for multiplex detection of serum sample components was prepared that could quantify the analytes glucose, potassium, and alkaline phosphatase (ALP). When serum samples were introduced by capillary action, the CPC sensor array showed fluorescence responses for each analyte and successfully identified the components with elevated concentrations in the serum samples.
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Fujii
authors.forename: Terence G
authors.initials: TG
authors.lastname: Henares
authors.forename: Kunio
authors.initials: K
authors.lastname: Kawamura
authors.forename: Tatsuro
authors.initials: T
authors.lastname: Endo
authors.forename: Hideaki
authors.initials: H
authors.lastname: Hisamoto
mesh_terms: D000469:Alkaline Phosphatase / Q000097:blood*; D001786:Blood Glucose / Q000032:analysis*; D003545:Cysteine / Q000737:chemistry; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D004867:Equipment Design; D006801:Humans; D046228:Microarray Analysis / Q000295:instrumentation*; D011188:Potassium / Q000097:blood*; D012680:Sensitivity and Specificity; D012834:Silver / Q000737:chemistry; D013499:Surface Properties
chemical_list: D001786:Blood Glucose; D004129:Dimethylpolysiloxanes; D012834:Silver; D000469:Alkaline Phosphatase; D003545:Cysteine; D011188:Potassium