title: Bilirubin oxidase from Bacillus pumilus: a promising enzyme for the elaboration of efficient cathodes in biofuel cells.
abstract: A CotA multicopper oxidase (MCO) from Bacillus pumilus, previously identified as a laccase, has been studied and characterized as a new bacterial bilirubin oxidase (BOD). The 59 kDa protein containing four coppers, was successfully over-expressed in Escherichia coli and purified to homogeneity in one step. This 509 amino-acid enzyme, having 67% and 26% sequence identity with CotA from Bacillus subtilis and BOD from Myrothecium verrucaria, respectively, shows higher turnover activity towards bilirubin compared to other bacterial MCOs. The current density for O(2) reduction, when immobilized in a redox hydrogel, is only 12% smaller than the current obtained with Trachyderma tsunodae BOD. Under continuous electrocatalysis, an electrode modified with the new BOD is more stable, and has a higher tolerance towards NaCl, than a T. tsunodae BOD modified electrode. This makes BOD from B. pumilus an attractive new candidate for application in biofuel cells (BFCs) and biosensors.
authors.affiliation: CRPP-UPR 8641, Univ. Bordeaux, F-33600, Pessac, France.
authors.forename: Fabien
authors.initials: F
authors.lastname: Durand
authors.affiliation: Department of Chemistry, Stanford University, Stanford, CA, 94305, USA.
authors.forename: Christian Hauge
authors.initials: CH
authors.lastname: Kjaergaard
authors.affiliation: CRPP-UPR 8641, Univ. Bordeaux, F-33600, Pessac, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Suraniti
authors.affiliation: CRPP-UPR 8641, Univ. Bordeaux, F-33600, Pessac, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Gounel
authors.affiliation: Department of Chemistry, Stanford University, Stanford, CA, 94305, USA.
authors.forename: Ryan G
authors.initials: RG
authors.lastname: Hadt
authors.affiliation: Department of Chemistry, Stanford University, Stanford, CA, 94305, USA.
authors.forename: Edward I
authors.initials: EI
authors.lastname: Solomon
authors.affiliation: CRPP-UPR 8641, Univ. Bordeaux, F-33600, Pessac, France. Electronic address: mano@crpp-bordeaux.cnrs.fr.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Mano
mesh_terms: D001407:Bacillus / Q000201:enzymology* / Q000235:genetics; D001674:Bioelectric Energy Sources / Q000382:microbiology; D055592:Biophysical Phenomena; D015374:Biosensing Techniques; D055664:Electrochemical Techniques; D004566:Electrodes; D004578:Electron Spin Resonance Spectroscopy; D004795:Enzyme Stability; D004800:Enzymes, Immobilized; D032902:Ganoderma / Q000201:enzymology; D005798:Genes, Bacterial; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D042845:Laccase / Q000235:genetics / Q000378:metabolism; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D004800:Enzymes, Immobilized; D010088:Oxidoreductases; D042845:Laccase; C024748:copper oxidase; D044925:Oxidoreductases Acting on CH-CH Group Donors; C042401:bilirubin oxidase