title: Chemical composition and in vitro antifungal activity screening of the Allium ursinum L. (Liliaceae).
abstract: The objective of the study was to summarize the methods for isolating and identifying natural sulfur compounds from Allium ursinum (ramson) and to discuss the active constituents with regard to antifungal action. Using chromatographic techniques, the active constituents were isolated and subsequently identified. Analyses by high-performance liquid chromatography (HPLC) suggested that these compounds were sulfur constituents, with a characteristic absorbance at 250 nm. Gas chromatography-mass spectrometry (GC-MS) analyses allowed the chemical structures of the isolated constituents to be postulated. We adopted the same methods to identify the health-giving profiling of ramsons and the effects are thought to be primarily derived from the presence and breakdown of the alk(en)ylcysteine sulphoxide, alliin and its subsequent breakdown to allicin (sulfur-compounds of ramson) in connection with antifungal action. The aim of the study was the characterization of the chemical composition of ramsons and the testing of the action of the in vitro extracts, on different strains of Candida albicans. The main goal was to highlight the most efficient extracts of Allium ursinum that can provide long-term antifungal activity without remissions. The extracts from Allium ursinum plants, inhibited growth of Candida spp. cells at concentrations ranging from 0.5 to 4.0 mg/mL, while that of adherent cells at concentrations ranging from 1.0 to > 4.0 mg/mL, depending on the yeast and plant species.
authors.affiliation: Department of Hygiene, University of Medicine and Pharmacy "Victor Babes" 2A Eftimie Murgu Square, Timisoara 300041, Romania.
authors.forename: Radu Vasile
authors.initials: RV
authors.lastname: Bagiu
authors.affiliation: Department of Hygiene, University of Medicine and Pharmacy "Victor Babes" 2A Eftimie Murgu Square, Timisoara 300041, Romania.
authors.forename: Brigitha
authors.initials: B
authors.lastname: Vlaicu
authors.affiliation: Chemistry and Vegetal Biochemistry, Banat's University of Agricultural Sciences and Veterinary Medicine from Timisoara, Calea Aradului no. 119, Timisoara 300645, Romania.
authors.forename: Monica
authors.initials: M
authors.lastname: Butnariu
mesh_terms: D000490:Allium / Q000737:chemistry*; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002176:Candida albicans / Q000254:growth & development*; D002851:Chromatography, High Pressure Liquid; D003545:Cysteine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug
chemical_list: D000935:Antifungal Agents; D000970:Antineoplastic Agents; C006453:alliin; D003545:Cysteine