title: Inhibition of acute experimental colitis by a crude extract and diets containing seeds of Garcinia kola (heckel).
abstract: The protective effect of Garcinia kola (GK) crude extract on acetic acid induced colitis in rats was investigated. Colitis, a form of inflammatory bowel disease (IBD) is characterized by inflammation of colon. The pathology in colitis includes disruption of crypt architecture, inflammation of crypts, frank crypt abscesses, and hemorrhage or inflammatory cells in the lamina propria. Since oxidative stress plays an important role in the etiology of Inflammatory Bowel diseases,and Garcinia kola (GK), have been shown to reduce oxidative stress in the rat stomach.The present study was designed to investigate the effects of Garcinia kola on ulcerative colitis (UC) induced by acetic acid. Albino rats were divided into five groups; Group one served as control, group two received Normal saline, group three received 2.5% ethanol while groups four and five were given 20mg/kg and 100mg/kg of crude extract of Garcinia kolarespectively. In another experiment, rats were fed for 2 weeks on normal rat diets but specially composed to contain 12.5%, 25% and 50% by weight of G kola. Colitis was induced by intra-rectal administration of 6% acetic acid. The colonic damage was elucidated by macroscopic damage scores; colon wet weight, stool consistency and colonic edema (thickness of the colon). Intra-luminal administration of 6% acetic acid resulted in observable clinical and macroscopic signs of colitis.Pre-orally administered of crude extract of GK significantly reduced the colonic damage score (p<0.001), colon weight (p<0.001), thickness of the colon (p<0.001) and diarrhea (p<0.001).Histological examinations also indicated a marked reduction in tissue injury and inhibition in neutrophil infiltration in rats pretreated with crude extract of Garcinia kola. Results of this investigation provide experimental evidence of Garcinia kola as an anti-colitis agent.
authors.affiliation: Department of Physiology, College of Medicine, University of Ibadan, Ibadan, Nigeria. sbolaleye@yahoo.com
authors.forename: S B
authors.initials: SB
authors.lastname: Olaleye
authors.forename: S F
authors.initials: SF
authors.lastname: Ige
authors.forename: S A
authors.initials: SA
authors.lastname: Onasanwo
authors.forename: C H
authors.initials: CH
authors.lastname: Cho
mesh_terms: D019342:Acetic Acid / Q000009:adverse effects; D000284:Administration, Oral; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D003093:Colitis, Ulcerative / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D003106:Colon / Q000378:metabolism / Q000473:pathology; D004032:Diet; D004195:Disease Models, Animal; D029763:Garcinia kola / Q000737:chemistry*; D008297:Male; D018384:Oxidative Stress; D010936:Plant Extracts / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012639:Seeds
chemical_list: D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D019342:Acetic Acid