title: A comparative analysis of the attachment of Leptospira interrogans and L. borgpetersenii to mammalian cells.
abstract: Leptospirosis, the world's most ubiquitous zoonosis, is caused by pathogenic Leptospira. As microbe-host interactions are specific in pathogenesis, it is likely that there are several molecules mediating the attachment of the Leptospira to mammalian cells. In this study, we analysed the attachment of Leptospira interrogans serovar Portlandvere and Leptospira borgpetersenii serovar Jules to untreated HEp-2 cells or HEp-2 cells treated with the various enzymes, lectins or sugars and to integrins αVβ3 and α5β1, relative to control wells. We found that both serovars bound equally well to HEp-2 cells; however, serovar Jules showed a higher level of attachment to integrins. Both serovars showed an increase in attachment to HEp-2 cells coated with lectins peanut agglutinin, Ulex europaeus agglutinin, soybean agglutinin and Erythrina cristagalli agglutinin (p < 0.05); in the case of Concanavalin A, Jules showed an increase, while Portlandvere showed a significant decrease in attachment. Trypsinizing monolayers resulted in a decrease in attachment for both serovars, while when chondroitinase, neuraminidase and heparinase were used an increase in attachment was recorded. Leptospires coated with sugars showed a decrease in attachment. These results show that serovar Jules' general greater affinity for the mediators examined may suggest a greater potential for virulence over serovar Portlandvere.
authors.affiliation: Basic Medical Sciences Department, Faculty of Medical Sciences, University of the West Indies, Kingston, Jamaica.
authors.forename: Gabrielle I
authors.initials: GI
authors.lastname: Andrade
authors.forename: Paul D
authors.initials: PD
authors.lastname: Brown
mesh_terms: D001422:Bacterial Adhesion; D050260:Carbohydrate Metabolism; D002460:Cell Line; D004798:Enzymes / Q000378:metabolism; D022781:Hepatocytes / Q000382:microbiology; D006801:Humans; D016023:Integrins / Q000378:metabolism; D037102:Lectins / Q000378:metabolism; D007919:Leptospira / Q000502:physiology*
chemical_list: D004798:Enzymes; D016023:Integrins; D037102:Lectins