title: Olmesartan attenuates the impairment of endothelial cells induced by oxidized low density lipoprotein through downregulating expression of LOX-1.
abstract: Oxidized low density lipoprotein (ox-LDL) and its receptor, lectin-Like ox-LDL receptor-1 (LOX-1), play important roles in the development of endothelial injuries. Olmesartan can protect endothelial cells from the impairment caused by various pathological stimulations. In the present study we investigated whether olmesartan decreased the impairment of endothelial cells induced by ox-LDL by exerting its effects on LOX-1 both in vitro and in vivo. Incubation of cultured endothelial cells of neonatal rats with ox-LDL for 24 h or infusion of ox-LDL in mice for 3 weeks led to the remarkable impairment of endothelial cells, including increased lactate dehydrogenase synthesis, phosphorylation of p38 mitogen-activated protein kinases (p38 MAPK) and expression of apoptotic genes such as B-cell leukemia/lymphoma 2 (Bcl-2)-associated X protein (Bax) and caspase-3. Simultaneously, the cell vitality and expression of Bcl-2 gene were greatly reduced. All these effects, however, were significantly suppressed by the treatment with olmesartan. Furthermore, ox-LDL promoted up-regulation of LOX-1 expression either in cultured endothelial cells or in the aortas of mice, which was reversed with the administration of olmesartan. Our data indicated that olmesartan may attenuate the impairment of endothelial cell via down-regulation of the increased LOX-1 expression induced by ox-LDL.
authors.affiliation: Department of Cardiology, Jiangsu Jiangyin People's Hospital, 163 Shoushan Road, Jiangyin, Jiangsu, 214400, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, Zhongda Hospital of Southeast University, 87 Dingjiqiao Hunan Road, Nanjing, Jiangsu, 210009, China.
authors.forename: Genshan
authors.initials: G
authors.lastname: Ma
authors.affiliation: Department of Cardiology, Zhongda Hospital of Southeast University, 87 Dingjiqiao Hunan Road, Nanjing, Jiangsu, 210009, China.
authors.forename: Yuyu
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Department of Cardiology, Jiangsu Jiangyin People's Hospital, 163 Shoushan Road, Jiangyin, Jiangsu, 214400, China.
authors.forename: Huidong
authors.initials: H
authors.lastname: Qian
authors.affiliation: Department of Cardiology, Jiangsu Jiangyin People's Hospital, 163 Shoushan Road, Jiangyin, Jiangsu, 214400, China.
authors.forename: Weizhang
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Cardiology, Jiangsu Jiangyin People's Hospital, 163 Shoushan Road, Jiangyin, Jiangsu, 214400, China.
authors.forename: Xinjun
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Cardiology, Jiangsu Jiangyin People's Hospital, 163 Shoushan Road, Jiangyin, Jiangsu, 214400, China.
authors.forename: Wenlong
authors.initials: W
authors.lastname: Jiang
authors.affiliation: Department of Cardiology, Jiangsu Jiangyin People's Hospital, 163 Shoushan Road, Jiangyin, Jiangsu, 214400, China.
authors.forename: Ruolong
authors.initials: R
authors.lastname: Zheng
mesh_terms: D047228:Angiotensin II Type 1 Receptor Blockers / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D015536:Down-Regulation / Q000187:drug effects*; D042783:Endothelial Cells / Q000378:metabolism* / Q000473:pathology; D007093:Imidazoles / Q000494:pharmacology*; D008077:Lipoproteins, LDL / Q000378:metabolism / Q000494:pharmacology*; D051379:Mice; D051381:Rats; D017207:Rats, Sprague-Dawley; D051127:Scavenger Receptors, Class E / Q000096:biosynthesis*; D013777:Tetrazoles / Q000494:pharmacology*
chemical_list: D047228:Angiotensin II Type 1 Receptor Blockers; D007093:Imidazoles; D008077:Lipoproteins, LDL; C495136:OLR1 protein, rat; C495138:Olr1 protein, mouse; D051127:Scavenger Receptors, Class E; D013777:Tetrazoles; C099252:oxidized low density lipoprotein; C437965:olmesartan