title: Label-free catalytic and molecular beacon containing an abasic site for sensitive fluorescent detection of small inorganic and organic molecules.
abstract: In this work, two methods with complementary features, catalytic and molecular beacon (CAMB) and label-free fluorescent sensors using an abasic site, have been combined into new label-free CAMB sensors that possess advantages of each method. The label-free method using a dSpacer-containing molecular beacon makes CAMB more cost-effective and less interfering with the catalytic activity, while CAMB allows the label-free method to use true catalytic turnovers for signal amplifications, resulting in a new label-free CAMB sensor for Pb(2+) ion, with a detection limit of 3.8 nM while maintaining the same selectivity. Furthermore, by using CAMB to overcome the label-free method's limitation of requiring excess enzyme strands, a new label-free CAMB sensor using aptazyme is also designed to detect adenosine down to 1.4 μM, with excellent selectivity over other nucleosides.
authors.affiliation: Department of Chemistry, Tsinghua University, Beijing, China.
authors.forename: Panshu
authors.initials: P
authors.lastname: Song
authors.forename: Yu
authors.initials: Y
authors.lastname: Xiang
authors.forename: Hang
authors.initials: H
authors.lastname: Xing
authors.forename: Zhaojuan
authors.initials: Z
authors.lastname: Zhou
authors.forename: Aijun
authors.initials: A
authors.lastname: Tong
authors.forename: Yi
authors.initials: Y
authors.lastname: Lu
mesh_terms: D000241:Adenosine / Q000032:analysis* / Q000097:blood; D052157:Aptamers, Nucleotide / Q000737:chemistry; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D002384:Catalysis; D002413:Cations, Divalent / Q000032:analysis; D021881:DNA, Catalytic / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D007854:Lead / Q000032:analysis*; D057230:Limit of Detection; D008969:Molecular Sequence Data; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D052157:Aptamers, Nucleotide; D002413:Cations, Divalent; D021881:DNA, Catalytic; D005456:Fluorescent Dyes; D007854:Lead; D000241:Adenosine