title: Evidence of unexpected oxidative stress in airways of adolescents born very pre-term.
abstract: Prematurity and its main respiratory complication, bronchopulmonary dysplasia (BPD), are potentially associated with lifelong respiratory morbidities and/or lung function abnormalities. The mechanisms behind these long-term respiratory problems are still unclear. We assessed airway oxidative stress in adolescents born very pre-term (≤ 32 gestational weeks) by measuring 8-isoprostane concentration in exhaled breath condensate (EBC). In addition, the study protocol included spirometry and measurement of exhaled nitric oxide fraction (F(eNO)). The study groups included 34 ex-pre-term adolescents with BPD, 18 ex-pre-term adolescents without BPD and 34 healthy controls born at term. Regardless of a history of BPD, the ex-premature adolescents had higher EBC 8-isoprostane levels (median (interquartile range) BPD 9.5 (7.3-12.2) pg·mL(-1); pre-term non-BPD 10 (8.1-16) pg·mL(-1)) than the controls (3.2 (1.9-6.5) pg·mL(-1)) (p<0.001). Forced expiratory volume in 1 s was lower in the BPD group (mean ± sd Z-score -2.1 ± 1.58) than in the pre-term non-BPD individuals (-1.13 ± 1.15), who showed in turn significantly lower values than the controls (0.18 ± 0.83; p<0.001). F(eNO) was similar in the three groups (p=0.55). Our data show that, after premature birth, evidence of oxidative stress in the airways may be detected into adolescence, suggesting that long-term respiratory abnormalities after pre-term birth may be associated with an ongoing airway disease and not just a stabilised structural lung damage.
authors.affiliation: Unit of Allergy and Respiratory Medicine University of Padua, Padua, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Filippone
authors.forename: Gea
authors.initials: G
authors.lastname: Bonetto
authors.forename: Massimo
authors.initials: M
authors.lastname: Corradi
authors.forename: Anna Chiara
authors.initials: AC
authors.lastname: Frigo
authors.forename: Eugenio
authors.initials: E
authors.lastname: Baraldi
mesh_terms: D000293:Adolescent; D015415:Biomarkers / Q000032:analysis; D001944:Breath Tests; D001997:Bronchopulmonary Dysplasia / Q000378:metabolism*; D015237:Dinoprost / Q000031:analogs & derivatives / Q000032:analysis; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature; D008297:Male; D018384:Oxidative Stress; D012137:Respiratory System / Q000378:metabolism*
chemical_list: D015415:Biomarkers; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost