title: Bystander-mediated stimulation of proteolipid protein-specific regulatory T (Treg) cells confers protection against experimental autoimmune encephalomyelitis (EAE) via TGF-β.
abstract: To assess the potency of regulatory T (Treg) cells induced against an irrelevant Ag, mice were orally vaccinated with Salmonella expressing Escherichia coli colonization factor antigen I fimbriae. Isolated CD25⁺ and CD25⁻CD4⁺ T cells were adoptively transferred to naive mice, and Treg cells effectively protected against experimental autoimmune encephalomyelitis (EAE), unlike Treg cells from Salmonella vector-immunized mice. This protection was abrogated upon in vivo neutralization of TGF-β, resulting in elevated IL-17 and loss of IL-4 and IL-10 production. Thus, Treg cells induced to irrelevant Ags offer a novel approach to treat autoimmune diseases independent of auto-Ag.
authors.affiliation: Department of Immunology and Infectious Diseases, Montana State University, P.O. Box 173610, Bozeman, MT 59717-3610, USA.
authors.forename: Sangmu
authors.initials: S
authors.lastname: Jun
authors.forename: Javier
authors.initials: J
authors.lastname: Ochoa-Repáraz
authors.forename: Dagmara
authors.initials: D
authors.lastname: Zlotkowska
authors.forename: Teri
authors.initials: T
authors.lastname: Hoyt
authors.forename: David W
authors.initials: DW
authors.lastname: Pascual
mesh_terms: D019264:Adoptive Transfer / Q000379:methods*; D000818:Animals; D024201:Bystander Effect / Q000276:immunology*; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000276:immunology; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000473:pathology / Q000628:therapy*; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D005260:Female; D016753:Interleukin-10 / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D020381:Interleukin-17 / Q000096:biosynthesis / Q000502:physiology; D015847:Interleukin-4 / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D051379:Mice; D008815:Mice, Inbred Strains; D018991:Myelin Proteolipid Protein / Q000276:immunology*; D061251:Primary Cell Culture; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000378:metabolism / Q000637:transplantation; D016212:Transforming Growth Factor beta / Q000037:antagonists & inhibitors / Q000502:physiology*
chemical_list: D018984:Epitopes, T-Lymphocyte; D020381:Interleukin-17; D018991:Myelin Proteolipid Protein; C047516:Plp1 protein, mouse; D016212:Transforming Growth Factor beta; D016753:Interleukin-10; D015847:Interleukin-4