title: Demonstration of microchimerism in pregnant sows and effects of congenital PRRSV infection.
abstract: The presence of foreign cells within the tissue/circulation of an individual is described as microchimerism. The main purpose of the present investigation was to study if microchimerism occurs in healthy sows/fetuses and if porcine reproductive and respiratory syndrome virus (PRRSV) infection influences this phenomenon. Six dams were inoculated intranasally with PRRSV and three non-inoculated dams served as controls. Male DNA was detected in female fetal sera of all dams via PCR. Male DNA was also detected in the maternal circulation. Sex-typing FISH showed the presence of male cells in the female fetal organs and vice versa. PRRSV infection did not influence microchimerism, but might misuse maternal and sibling microchimeric cells to enter fetuses.
authors.affiliation: Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, Ghent, Belgium. uladzimir.karniychuk@UGent.be.
authors.forename: Uladzimir U
authors.initials: UU
authors.lastname: Karniychuk
authors.forename: Wander
authors.initials: W
authors.lastname: Van Breedam
authors.forename: Nadine
authors.initials: N
authors.lastname: Van Roy
authors.forename: Claire
authors.initials: C
authors.lastname: Rogel-Gaillard
authors.forename: Hans J
authors.initials: HJ
authors.lastname: Nauwynck
mesh_terms: D000818:Animals; D046528:Chimerism / Q000662:veterinary*; D004247:DNA / Q000097:blood*; D005260:Female; D017404:In Situ Hybridization, Fluorescence / Q000662:veterinary; D008297:Male; D016133:Polymerase Chain Reaction / Q000662:veterinary; D019318:Porcine Reproductive and Respiratory Syndrome / Q000503:physiopathology* / Q000821:virology; D019316:Porcine respiratory and reproductive syndrome virus / Q000502:physiology*; D011247:Pregnancy; D013552:Swine / Q000235:genetics*
chemical_list: D004247:DNA