title: Sea buckthorn (Hippophae rhamnoides) proanthocyanidins inhibit in vitro enzymatic hydrolysis of protein.
abstract: Interactions of phenolics with other food constituents and digestive enzymes are likely to have interference with the digestion and bioavailability of food and phenolics. In this study the effect of sea buckthorn proanthocyanidins on in vitro digestion of protein was evaluated. Optimization of the extraction conditions showed that maximum recovery of sea buckthorn proanthocyanidins was obtained with acidified acetone; water mixture (60% to 70%, v/v). Crude proanthocyanidin extracts thus prepared were purified using sephadex gel column chromatography and their average degree of polymerization and the effects on enzymatic hydrolysis of bovine serum albumin as influenced by their protein precipitation capacities were studied. Average degree of polymerization of proanthocyanidins in berry pulp, kernel, seed coat, and leaves was 7.4, 5.6, 8.2, and 10.6, respectively. The EC50 values for the protein precipitation by the PA of berry pulp, kernel seed coat, and leaves were 44.2, 44.1, 65.8, and 39.8 μg, respectively. Relative enzymatic hydrolysis of the protein-proanthocyanidin complexes was 44.1% to 60.3% for pepsin and 57.5% to 67.7% for trypsin. Interactions of sea buckthorn proanthocyanidins with food proteins and digestive enzymes might alter the protein digestibility and phenolic bioavailabilty.
authors.affiliation: Agroprocessing and Natural Products Division, Natl. Inst. for Interdisciplinary Science and Technology (CSIR), Thiruvananthapuram, Kerala, India, 695019.
authors.forename: Ranjith
authors.initials: R
authors.lastname: Arimboor
authors.forename: C
authors.initials: C
authors.lastname: Arumughan
mesh_terms: D011232:Chemical Precipitation; D004044:Dietary Proteins / Q000378:metabolism*; D004063:Digestion / Q000187:drug effects*; D005638:Fruit / Q000009:adverse effects / Q000737:chemistry*; D031281:Hippophae / Q000009:adverse effects / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis / Q000187:drug effects; D007194:India; D008970:Molecular Weight; D010434:Pepsin A / Q000037:antagonists & inhibitors / Q000378:metabolism; D010636:Phenols / Q000032:analysis; D019441:Plant Epidermis / Q000009:adverse effects / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018515:Plant Leaves / Q000009:adverse effects / Q000737:chemistry; D044945:Proanthocyanidins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011480:Protease Inhibitors / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D012639:Seeds / Q000009:adverse effects / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism; D014357:Trypsin / Q000737:chemistry / Q000378:metabolism; D014361:Trypsin Inhibitors / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D004044:Dietary Proteins; D010636:Phenols; D010936:Plant Extracts; D044945:Proanthocyanidins; D011480:Protease Inhibitors; D014361:Trypsin Inhibitors; D012710:Serum Albumin, Bovine; D014357:Trypsin; D010434:Pepsin A