title: Effect of pre-freezing conditions on the progressive motility recovery rate of human frozen spermatozoa.
abstract: We evaluated the effects of sperm concentration, progressive motility, sperm morphology, duration of abstinence and collection season on the progressive motility recovery rate of human frozen spermatozoa to identify characteristics that predict the progressive motility recovery rate of human frozen spermatozoa and improve the protocol for sperm collecting in sperm banks. A total of 14 190 semen samples donated at Zhejiang human sperm bank of China between September 2006 and June 2011 were collected from 1624 donors. Semen was evaluated according to WHO standard procedures for sperm concentration. Progressive motility, sperm morphology, ejaculate collection season and abstinence time were recorded. After freezing and thawing, the progressive motility was assessed. Results showed that sperm concentration, progressive motility and normal morphology were significantly associated with the progressive motility recovery rate of human frozen spermatozoa. In addition, the abstinence time and collection season also significantly affected progressive motility recovery rate. Our results indicated that sperm concentration, progressive motility and normal morphology could be valuable in predicting the progressive motility recovery rate of human frozen spermatozoa. As such, progressive motility recovery may be improved by donating semen when abstinent for 3-5 days and during seasons other than summer.
authors.affiliation: Family Planning Research Institute, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.forename: Y
authors.initials: Y
authors.lastname: Zhou
authors.forename: W
authors.initials: W
authors.lastname: Xia
authors.forename: H
authors.initials: H
authors.lastname: Wu
authors.forename: K
authors.initials: K
authors.lastname: Yao
authors.forename: H
authors.initials: H
authors.lastname: Liu
authors.forename: C
authors.initials: C
authors.lastname: Xiong
mesh_terms: D000328:Adult; D044466:Asian People; D015925:Cryopreservation / Q000379:methods*; D005615:Freezing; D006801:Humans; D008297:Male; D012621:Seasons; D055101:Semen Analysis; D012662:Semen Preservation / Q000379:methods*; D012746:Sexual Abstinence; D013081:Sperm Motility; D013094:Spermatozoa / Q000166:cytology*