title: On-site chemical reaction lights up protein assemblies in cells.
abstract: Here we report a fast and effective method to visualize interactive proteins across intact mammalian cells via on-site formation of fluorescence using instant reaction of non-fluorescent fluorescamine with primary amines on proteins. Without interference by fluorescence background, this fluorogenic labelling opens a way for selective identification of primary amine-rich interacting proteins, efficient mapping and real-time monitoring of their spatial distribution in assemblies/network, and fast differentiation of cellular types. Without adverse effect on biological functions, this labelling method also provides new insights to comprehend important aspects of cellular functions of organelles and their relation to health imperfections for disease diagnostics and imaging-guided therapy.
authors.affiliation: Institute of Materials Research and Engineering, A*STAR (Agency for Science, Technology and Research), 3 Research Link, Singapore 117602.
authors.forename: Khin Yin
authors.initials: KY
authors.lastname: Win
authors.forename: Choon Peng
authors.initials: CP
authors.lastname: Teng
authors.forename: Michelle Low Bee
authors.initials: ML
authors.lastname: Jin
authors.forename: Enyi
authors.initials: E
authors.lastname: Ye
authors.forename: Natalia C
authors.initials: NC
authors.lastname: Tansil
authors.forename: Ming-Yong
authors.initials: MY
authors.lastname: Han
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D005450:Fluorescamine / Q000737:chemistry; D006801:Humans; D018613:Microscopy, Confocal; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D051381:Rats
chemical_list: D011506:Proteins; D005450:Fluorescamine