title: Detection and kinetic analysis of Epinotia aporema granulovirus in its lepidopteran host by real-time PCR.
abstract: Epinotia aporema granulovirus (EpapGV) has attracted interest as a potential biocontrol agent of the soybean pest Epinotia aporema in Argentina. Studies on virus/host interactions conducted so far have lacked an accurate method to assess the progress of virus load during the infection process. The present paper reports the development of a real-time PCR for EpapGV and its application to describe viral kinetics following ingestion of two different virus doses by last-instar E. aporema larvae. Real-time PCR was shown to be a reliable method to detect and quantify the presence of EpapGV in the analyzed samples. The increase in virus titer (log) exhibited a sigmoidal pattern, with an exponential growth phase between 24 and 48 h postinfection for both initial doses tested.
authors.affiliation: Instituto de Microbiología y Zoología Agrícola, INTA Castelar, Argentina. arneodoj@yahoo.com
authors.forename: J D
authors.initials: JD
authors.lastname: Arneodo
authors.forename: G A
authors.initials: GA
authors.lastname: König
authors.forename: M F
authors.initials: MF
authors.lastname: Berretta
authors.forename: J A
authors.initials: JA
authors.lastname: Di Rienzo
authors.forename: O
authors.initials: O
authors.lastname: Taboga
authors.forename: A
authors.initials: A
authors.lastname: Sciocco-Cap
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D007700:Kinetics; D007915:Lepidoptera / Q000821:virology*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D014764:Viral Proteins