title: Aliphatic (1)H, (13)C and (15)N chemical shift assignments of dihydrofolate reductase from the psychropiezophile Moritella profunda in complex with NADP(+) and folate.
abstract: Dihydrofolate reductase from the deep-sea bacterium Moritella profunda (MpDHFR) has been (13)C/(15)N isotopically labelled and purified. Here, we report the aliphatic (1)H, (13)C and (15)N resonance assignments of MpDHFR in complex with NADP(+) and folate. The spectra of MpDHFR suggest considerably greater conformational heterogeneity than is seen in the closely related DHFR from Escherichia coli.
authors.affiliation: School of Chemistry, Cardiff University, Park Place, Cardiff, CF10 3AT, UK.
authors.forename: E Joel
authors.initials: EJ
authors.lastname: Loveridge
authors.forename: Stella M
authors.initials: SM
authors.lastname: Matthews
authors.forename: Christopher
authors.initials: C
authors.lastname: Williams
authors.forename: Sara B-M
authors.initials: SB
authors.lastname: Whittaker
authors.forename: Ulrich L
authors.initials: UL
authors.lastname: Günther
authors.forename: Rhiannon M
authors.initials: RM
authors.lastname: Evans
authors.forename: William M
authors.initials: WM
authors.lastname: Dawson
authors.forename: Matthew P
authors.initials: MP
authors.lastname: Crump
authors.forename: Rudolf K
authors.initials: RK
authors.lastname: Allemann
mesh_terms: D005492:Folic Acid / Q000378:metabolism*; D034221:Moritella / Q000201:enzymology*; D009249:NADP / Q000378:metabolism*; D019906:Nuclear Magnetic Resonance, Biomolecular; D013762:Tetrahydrofolate Dehydrogenase / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D009249:NADP; D005492:Folic Acid; D013762:Tetrahydrofolate Dehydrogenase