title: [Clinical significance of detecting partial cytokines in serum of patients with pulmonary tuberculosis by cytometric bead array].
abstract: OBJECTIVE
  To explore the clinical significance of cytometric bead array(CBA) in detecting partial cytokines(IL-2,  IL-4,  IL-6,  IL-10,  TNF-α and IFN-γ)in the serum from patients with different types of pulmonary tuberculosis.
  
  
  METHODS
  The levels of six cytokines (IL-2,  IL-4,  IL-6,  IL-10,  TNF-α and IFN-γ) in the serum from 84 cases of patients with pulmonary tuberculosis(46 cases of active pulmonary tuberculosis patients,  38 cases of inactive pulmonary tuberculosis patients) and 30 cases of normal person,   were detected by BD CBA Flex Set kit.
  
  
  RESULTS
  The levels of IL-2, IL-4, IL-6, IL-10, TNF-α and IFN-γ in pulmonary tuberculosis group were significantly higher than those in control group (P<0.01 or P<0.05.Compared with the inactive pulmonary tuberculosis group, the levels of serum IL-2, IL-4, IL-6, IL-10, TNF-α and IFN-γ in the active pulmonary tuberculosis group were significantly higher (P<0.01 or P<0.05).Serum IL-4 and TNF-α levels in active pulmonary tuberculosis group,  inactive pulmonary tuberculosis group and the control group had not significantly different.
  
  
  CONCLUSIONS
  Six  cytokines in serum  can be detected    quickly and sensitively in batches at one   time  by CBA. IL-2, IL-4, IL-6, IL-10, TNF-α and IFN-γ play an important role in the immune pathogenesis of pulmonary tuberculosis.
authors.affiliation: Department of Microbiology and Immunology, Kunming Medical College, Kunming, China.
authors.forename: Bing-xue
authors.initials: BX
authors.lastname: Li
authors.forename: Xin-ya
authors.initials: XY
authors.lastname: Duan
authors.forename: Ming-wu
authors.initials: MW
authors.lastname: Li
authors.forename: Xiao-fei
authors.initials: XF
authors.lastname: Li
authors.forename: Qun
authors.initials: Q
authors.lastname: Zhao
authors.forename: Ya-ling
authors.initials: YL
authors.lastname: Wang
authors.forename: Ai-hua
authors.initials: AH
authors.lastname: Liu
authors.forename: Fu-kai
authors.initials: FK
authors.lastname: Bao
mesh_terms: D000293:Adolescent; D000328:Adult; D001774:Blood Chemical Analysis / Q000295:instrumentation*; D016207:Cytokines / Q000097:blood*; D005260:Female; D005434:Flow Cytometry / Q000295:instrumentation*; D006801:Humans; D008297:Male; D008863:Microspheres; D008875:Middle Aged; D014397:Tuberculosis, Pulmonary / Q000097:blood* / Q000276:immunology; D055815:Young Adult
chemical_list: D016207:Cytokines