title: Repetitive intermittent hypoxia induces respiratory and somatic motor recovery after chronic cervical spinal injury.
abstract: Spinal injury disrupts connections between the brain and spinal cord, causing life-long paralysis. Most spinal injuries are incomplete, leaving spared neural pathways to motor neurons that initiate and coordinate movement. One therapeutic strategy to induce functional motor recovery is to harness plasticity in these spared neural pathways. Chronic intermittent hypoxia (CIH) (72 episodes per night, 7 nights) increases synaptic strength in crossed spinal synaptic pathways to phrenic motoneurons below a C2 spinal hemisection. However, CIH also causes morbidity (e.g., high blood pressure, hippocampal apoptosis), rendering it unsuitable as a therapeutic approach to chronic spinal injury. Less severe protocols of repetitive acute intermittent hypoxia may elicit plasticity without associated morbidity. Here we demonstrate that daily acute intermittent hypoxia (dAIH; 10 episodes per day, 7 d) induces motor plasticity in respiratory and nonrespiratory motor behaviors without evidence for associated morbidity. dAIH induces plasticity in spared, spinal pathways to respiratory and nonrespiratory motor neurons, improving respiratory and nonrespiratory (forelimb) motor function in rats with chronic cervical injuries. Functional improvements were persistent and were mirrored by neurochemical changes in proteins that contribute to respiratory motor plasticity after intermittent hypoxia (BDNF and TrkB) within both respiratory and nonrespiratory motor nuclei. Collectively, these studies demonstrate that repetitive acute intermittent hypoxia may be an effective and non-invasive means of improving function in multiple motor systems after chronic spinal injury.
authors.affiliation: Department of Comparative Biosciences, University of Wisconsin, Madison, Wisconsin 53706, USA.
authors.forename: Mary R
authors.initials: MR
authors.lastname: Lovett-Barr
authors.forename: Irawan
authors.initials: I
authors.lastname: Satriotomo
authors.forename: Gillian D
authors.initials: GD
authors.lastname: Muir
authors.forename: Julia E R
authors.initials: JE
authors.lastname: Wilkerson
authors.forename: Michael S
authors.initials: MS
authors.lastname: Hoffman
authors.forename: Stéphane
authors.initials: S
authors.lastname: Vinit
authors.forename: Gordon S
authors.initials: GS
authors.lastname: Mitchell
mesh_terms: D000818:Animals; D002574:Cervical Vertebrae; D002908:Chronic Disease; D000860:Hypoxia / Q000503:physiopathology*; D008297:Male; D009046:Motor Neurons / Q000502:physiology*; D009473:Neuronal Plasticity / Q000502:physiology; D010791:Phrenic Nerve / Q000502:physiology*; D051381:Rats; D011917:Rats, Inbred Lew; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000502:physiology*; D015656:Respiratory Mechanics / Q000502:physiology*; D013119:Spinal Cord Injuries / Q000503:physiopathology / Q000628:therapy*; D013997:Time Factors