title: Synthesis and characterization of Fe3O4@SiO2 core-shell magnetic microspheres for extraction of genomic DNA from human whole blood.
abstract: An improved procedure was described for preparation of monodisperse Fe3O4@SiO2 core-shell magnetic microspheres via solvothermal method followed by a modified Stöber process. The magnetic composite microspheres were characterized with TEM, SEM, FTIR and VSM. Genomic DNA was then extracted from human whole blood using the as synthesized magnetic microspheres on the Eppendorf epMotion5075 workstation. The quality of eluted DNA was evaluated by PCR. The method was very expeditious without using toxic compounds such as phenol or chloroform and can be used for further molecular biology experiments.
authors.affiliation: School of Chemistry and Chemical Engineering, Southeast University, Nanjing, 211189, PR China.
authors.forename: Guopeng
authors.initials: G
authors.lastname: Li
authors.forename: Bin
authors.initials: B
authors.lastname: Shen
authors.forename: Nongyue
authors.initials: N
authors.lastname: He
authors.forename: Chao
authors.initials: C
authors.lastname: Ma
authors.forename: Sauli
authors.initials: S
authors.lastname: Elingarami
authors.forename: Zhiyang
authors.initials: Z
authors.lastname: Li
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000097:blood* / Q000302:isolation & purification; D017931:DNA Primers; D004587:Electrophoresis, Agar Gel; D005290:Ferric Compounds / Q000737:chemistry*; D006801:Humans; D008280:Magnetics; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008863:Microspheres; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012822:Silicon Dioxide / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D017931:DNA Primers; D005290:Ferric Compounds; C000499:ferric oxide; D012822:Silicon Dioxide; D004247:DNA