title: Two unsaturated fatty acids with potent α-glucosidase inhibitory activity purified from the body wall of sea cucumber (Stichopus japonicus).
abstract: One therapeutic approach for preventing diabetes mellitus and obesity is to retard the absorption of glucose via inhibition of α-glucosidase. Two unsaturated fatty acids with strong α-glucosidase inhibitory activity, 7(Z)-octadecenoic acid (1) and 7(Z),10(Z)-octadecadienoic acid (2), were purified from the body wall of Stichopus japonicus. IC(50) values of compounds 1 and 2 were 0.51 and 0.67 μg/mL against Saccharomyces cerevisiae α-glucosidase and 0.49 and 0.60 μg/mL against Bacillus stearothermophilus α-glucosidase, respectively. These compounds mildly inhibited rat-intestinal sucrase and maltase. In addition, both compounds showed a mixed type of inhibition against S. cerevisiae α-glucosidase and were very stable under thermal and acidic conditions up to 60 min. The K(I) and K(IS) values of compounds 1 and 2 were 0.44 and 0.22 μg/mL, and 0.39 and 0.13 μg/mL, respectively.
  
  
  CONCLUSIONS
  One therapeutic approach for preventing diabetes mellitus is to retard the absorption of glucose via inhibition of α-glucosidase. In this study, 2 fatty acids with strong α-glucosidase-inhibitory activity, 7(Z)-octadecenoic acid and 7(Z),10(Z)-octadecadienoic acid, were purified and identified from sea cucumber. Therefore, sea cucumber fatty acids can potentially be developed as a novel natural nutraceutical for the management of type-2 diabetes.
authors.affiliation: Dept. of Marine Food Science and Technology, Gangneung-Wonju National Univ., Gangneung 210-702, Republic of Korea.
authors.forename: The Han
authors.initials: TH
authors.lastname: Nguyen
authors.forename: Byung Hun
authors.initials: BH
authors.lastname: Um
authors.forename: Sang Moo
authors.initials: SM
authors.lastname: Kim
mesh_terms: D000818:Animals; D003920:Diabetes Mellitus / Q000517:prevention & control; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D001411:Geobacillus stearothermophilus / Q000201:enzymology; D065089:Glycoside Hydrolase Inhibitors; D020128:Inhibitory Concentration 50; D009829:Oleic Acids / Q000494:pharmacology*; D051381:Rats; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D047528:Stichopus / Q000737:chemistry*; D013393:Sucrase / Q000037:antagonists & inhibitors / Q000378:metabolism; D000520:alpha-Glucosidases / Q000378:metabolism
chemical_list: D005231:Fatty Acids, Unsaturated; D065089:Glycoside Hydrolase Inhibitors; D009829:Oleic Acids; C027369:octadecadienoic acid; D000520:alpha-Glucosidases; D013393:Sucrase