title: Analysis of clinical ostreid herpesvirus 1 (Malacoherpesviridae) specimens by sequencing amplified fragments from three virus genome areas.
abstract: Although there are a number of ostreid herpesvirus 1 (OsHV-1) variants, it is expected that the true diversity of this virus will be known only after the analysis of significantly more data. To this end, we analyzed 72 OsHV-1 "specimens" collected mainly in France over an 18-year period, from 1993 to 2010. Additional samples were also collected in Ireland, the United States, China, Japan, and New Zealand. Three virus genome regions (open reading frame 4 [ORF4], ORF35, -36, -37, and -38, and ORF42 and -43) were selected for PCR analysis and sequencing. Although ORF4 appeared to be the most polymorphic genome area, distinguishing several genogroups, ORF35, -36, -37, and -38 and ORF42 and -43 also showed variations useful in grouping subpopulations of this virus.
authors.affiliation: Institut Français pour la Recherche et l'Exploitation de la Mer, Laboratoire de Génétique et de Pathologie, Ronce les Bains, La Tremblade, France. Tristan.Renault@ifremer.fr
authors.forename: Tristan
authors.initials: T
authors.lastname: Renault
authors.forename: Pierrick
authors.initials: P
authors.lastname: Moreau
authors.forename: Nicole
authors.initials: N
authors.lastname: Faury
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Pepin
authors.forename: Amélie
authors.initials: A
authors.lastname: Segarra
authors.forename: Stephen
authors.initials: S
authors.lastname: Webb
mesh_terms: D000818:Animals; D004267:DNA Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010125:Ostreidae / Q000821:virology*; D010802:Phylogeny; D017422:Sequence Analysis, DNA