title: Clinical Neisseria gonorrhoeae isolate with a N. meningitidis porA gene and no prolyliminopeptidase activity, Sweden, 2011: danger of false-negative genetic and culture diagnostic results.
abstract: We describe a Neisseria gonorrhoeae strain, found in Sweden in 2011, that harbours a N. meningitidis porA gene causing false-negative results in PCRs targeting the gonococcal porA pseudogene. Furthermore, the strain had no prolyliminopeptidase (PIP) activity that many commercial biochemical kits for species verification in culture rely on. Enhanced awareness of the spread of such strains and screening for them can be crucial.
authors.affiliation: WHO Collaborating Centre for Gonorrhoea and other STIs, Swedish Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Microbiology, Orebro University Hospital, Orebro, Sweden.
authors.forename: D
authors.initials: D
authors.lastname: Golparian
authors.forename: E
authors.initials: E
authors.lastname: Johansson
authors.forename: M
authors.initials: M
authors.lastname: Unemo
mesh_terms: D000328:Adult; D000626:Aminopeptidases / Q000235:genetics* / Q000378:metabolism; D018929:Cell Culture Techniques; D004789:Enzyme Activation; D005188:False Negative Reactions; D005260:Female; D006069:Gonorrhea / Q000175:diagnosis / Q000235:genetics / Q000382:microbiology; D006801:Humans; D009344:Neisseria gonorrhoeae / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification*; D009345:Neisseria meningitidis / Q000235:genetics* / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods; D018272:Porins / Q000235:genetics*; D011544:Pseudogenes / Q000235:genetics*; D013548:Sweden
chemical_list: D018272:Porins; C057699:porin protein, Neisseria; D000626:Aminopeptidases; C022450:prolyl aminopeptidase