title: Laminin active peptide/agarose matrices as multifunctional biomaterials for tissue engineering.
abstract: Cell adhesive peptides derived from extracellular matrix components are potential candidates to afford bio-adhesiveness to cell culture scaffolds for tissue engineering. Previously, we covalently conjugated bioactive laminin peptides to polysaccharides, such as chitosan and alginate, and demonstrated their advantages as biomaterials. Here, we prepared functional polysaccharide matrices by mixing laminin active peptides and agarose gel. Several laminin peptide/agarose matrices showed cell attachment activity. In particular, peptide AG73 (RKRLQVQLSIRT)/agarose matrices promoted strong cell attachment and the cell behavior depended on the stiffness of agarose matrices. Fibroblasts formed spheroid structures on the soft AG73/agarose matrices while the cells formed a monolayer with elongated morphologies on the stiff matrices. On the stiff AG73/agarose matrices, neuronal cells extended neuritic processes and endothelial cells formed capillary-like networks. In addition, salivary gland cells formed acini-like structures on the soft matrices. These results suggest that the peptide/agarose matrices are useful for both two- and three-dimensional cell culture systems as a multifunctional biomaterial for tissue engineering.
authors.affiliation: Laboratory of Clinical Biochemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Yamada
authors.forename: Kentaro
authors.initials: K
authors.lastname: Hozumi
authors.forename: Akihiro
authors.initials: A
authors.lastname: Aso
authors.forename: Atsushi
authors.initials: A
authors.lastname: Hotta
authors.forename: Kazunori
authors.initials: K
authors.lastname: Toma
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Katagiri
authors.forename: Yamato
authors.initials: Y
authors.lastname: Kikkawa
authors.forename: Motoyoshi
authors.initials: M
authors.lastname: Nomizu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001672:Biocompatible Materials; D002448:Cell Adhesion; D002478:Cells, Cultured; D004492:Edetic Acid; D006493:Heparin; D006801:Humans; D007797:Laminin / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D016501:Neurites; D016716:PC12 Cells; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D012685:Sepharose / Q000378:metabolism*; D023822:Tissue Engineering
chemical_list: D001672:Biocompatible Materials; D007797:Laminin; D010455:Peptides; D006493:Heparin; D012685:Sepharose; D004492:Edetic Acid