title: A novel totivirus-like virus isolated from bat guano.
abstract: Previous metagenomic analysis indicated that numerous insect viruses exist in bat guano. In this study, we isolated a novel double-stranded RNA virus, a tentative member of the family Totiviridae, designated Tianjin totivirus (ToV-TJ), from bat feces. The virus is an icosahedral particle with a diameter of 40-43 nm, and it causes cytopathic effect in Sf9, Hz, and C6/36 cell lines. Full-length genomic sequence analysis showed that ToV-TJ shares high similarity with the totivirus OMRV-AK4, which was recently isolated from mosquitoes in Japan. The full-length genome of the ToV-TJ was 7611 bp and contained two predicted non-overlapping open reading frames (ORFs): ORF1, encoding the capsid protein (CP), and ORF2, encoding an RNA-dependent RNA polymerase. Bioassay of ToV-TJ by feeding on the larvae of Spodoptera exigua and Helicoverpa armigera (Hubner) suggests that this virus is not infectious for these two larvae in vivo. Sequences similar to that of ToV-TJ have been detected in bat feces sampled in Yunnan and Hainan Provinces, suggesting that this virus is widely distributed.
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, China.
authors.forename: Xinglou
authors.initials: X
authors.lastname: Yang
authors.forename: Yunzhi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xingyi
authors.initials: X
authors.lastname: Ge
authors.forename: Junfa
authors.initials: J
authors.lastname: Yuan
authors.forename: Zhengli
authors.initials: Z
authors.lastname: Shi
mesh_terms: D000818:Animals; D002460:Cell Line; D002681:China; D002685:Chiroptera / Q000821:virology*; D005243:Feces / Q000821:virology; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D019196:Totiviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins