title: Mitochondrial protection and anti-aging activity of Astragalus polysaccharides and their potential mechanism.
abstract: The current study was performed to investigate mitochondrial protection and anti-aging activity of Astragalus polysaccharides (APS) and the potential underlying mechanism. Lipid peroxidation of liver and brain mitochondria was induced by Fe(2+)-Vit C in vitro. Thiobarbituric acid (TBA) colorimetry was used to measure the content of thiobarbituric acid reactive substances (TBARS). Mouse liver mitochondrial permeability transition (PT) was induced by calcium overload in vitro and spectrophotometry was used to measure it. The scavenging activities of APS on superoxide anion (O(2) (•-)) and hydroxyl radical (•OH), which were produced by reduced nicotinamide adenine dinucleotide (NADH)-N-Methylphenazonium methyl sulfate (PMS) and hydrogen peroxide (H(2)O(2))-Fe(2+) system respectively, were measured by 4-nitrobluetetrazolium chloride (NBT) reduction and Fenton reaction colorimetry respectively. The Na(2)S(2)O(3) titration method was used to measure the scavenging activities of APS on H(2)O(2). APS could inhibit TBARS production, protect mitochondria from PT, and scavenge O(2) (•-), •OH and H(2)O(2) significantly in a concentration-dependent manner respectively. The back of the neck of mice was injected subcutaneously with D-galactose to induce aging at a dose of 100 mg/kg/d for seven weeks. Moreover, the activities of catalase (CAT), surperoxide dismutase (SOD) and glutathione peroxidase (GPx) and anti-hydroxyl radical which were assayed by using commercial monitoring kits were increased significantly in vivo by APS. According to this research, APS protects mitochondria by scavenging reactive oxygen species (ROS), inhibiting mitochondrial PT and increasing the activities of antioxidases. Therefore, APS has the effect of promoting health.
authors.affiliation: College of Life Science, Dalian Nationalities University, No.18 Liaohe West Road, Dalian Economic & Technical Development Zone, Dalian 116600, China.
authors.forename: Xing-Tai
authors.initials: XT
authors.lastname: Li
authors.affiliation: Daxing'anling Academy of Agriculture and Forestry Sciences, Jiagedaqi 165000, China.
authors.forename: Ya-Kui
authors.initials: YK
authors.lastname: Zhang
authors.affiliation: College of Pharmacy, Heilongjiang University of Traditional Chinese Medicine, Harbin 150040, China.
authors.forename: Hai-Xue
authors.initials: HX
authors.lastname: Kuang
authors.affiliation: Daxing'anling Academy of Agriculture and Forestry Sciences, Jiagedaqi 165000, China.
authors.forename: Feng-Xin
authors.initials: FX
authors.lastname: Jin
authors.affiliation: Daxing'anling Academy of Agriculture and Forestry Sciences, Jiagedaqi 165000, China.
authors.forename: De-Wen
authors.initials: DW
authors.lastname: Liu
authors.affiliation: College of Life Science, Dalian Nationalities University, No.18 Liaohe West Road, Dalian Economic & Technical Development Zone, Dalian 116600, China.
authors.forename: Ming-Bo
authors.initials: MB
authors.lastname: Gao
authors.affiliation: College of Life Science, Dalian Nationalities University, No.18 Liaohe West Road, Dalian Economic & Technical Development Zone, Dalian 116600, China.
authors.forename: Ze
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Daxing'anling Academy of Agriculture and Forestry Sciences, Jiagedaqi 165000, China.
authors.forename: Xiao-Juan
authors.initials: XJ
authors.lastname: Xin
mesh_terms: D000818:Animals; D027883:Astragalus Plant / Q000737:chemistry*; D001921:Brain / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D002463:Cell Membrane Permeability / Q000187:drug effects; D016922:Cellular Senescence / Q000187:drug effects*; D019610:Cytoprotection / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D051336:Mitochondrial Membranes / Q000187:drug effects / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D028321:Plant Preparations / Q000494:pharmacology*; D011134:Polysaccharides / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D028321:Plant Preparations; D011134:Polysaccharides; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances