title: Comparison of the convergent receptor utilization of a retargeted feline leukemia virus envelope with a naturally-occurring porcine endogenous retrovirus A.
abstract: In vitro screening of randomized FeLV Envelope libraries identified the CP isolate, which enters cells through HuPAR-1, one of two human receptors utilized by porcine endogenous retrovirus-A (PERV-A), a distantly related gammaretrovirus. The CP and PERV-A Envs however, share little amino acid homology. Their receptor utilization was examined to define the common receptor usage of these disparate viral Envs. We demonstrate that the receptor usage of CP extends to HuPAR-2 but not to the porcine receptor PoPAR, the cognate receptor for PERV-A. Reciprocal interference between virus expressing CP and PERV-A Envs was observed on human cells. Amino acid residues localized to within the putative second extracellular loop (ECL-2) of PAR-1 and PAR-2 are found to be critical for CP envelope function. Through a panel of receptor chimeras and point mutations, this area was also found to be responsible for the differential usage of the PoPAR receptor between CP and PERV-A.
authors.affiliation: University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Department of Biochemistry, Piscataway, NJ 08854, USA. mazaripm@umdnj.edu
authors.forename: Peter M
authors.initials: PM
authors.lastname: Mazari
authors.forename: Takele
authors.initials: T
authors.lastname: Argaw
authors.forename: Leonardo
authors.initials: L
authors.lastname: Valdivieso
authors.forename: Xia
authors.initials: X
authors.lastname: Zhang
authors.forename: Katherine T
authors.initials: KT
authors.lastname: Marcucci
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Salomon
authors.forename: Carolyn A
authors.initials: CA
authors.lastname: Wilson
authors.forename: Monica J
authors.initials: MJ
authors.lastname: Roth
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002415:Cats; D002460:Cell Line; D020077:Endogenous Retroviruses / Q000378:metabolism*; D015967:Gene Expression Regulation, Viral; D006801:Humans; D005256:Leukemia Virus, Feline / Q000502:physiology*; D009154:Mutation; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000502:physiology*; D011994:Recombinant Proteins; D013552:Swine; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D056189:Viral Tropism; D053586:Virus Internalization
chemical_list: D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins