title: Green tea prevents obesity by increasing expression of insulin-like growth factor binding protein-1 in adipose tissue of high-fat diet-fed mice.
abstract: It is known that green tea has the ability to prevent obesity, but the underlying molecular mechanism is not fully understood to date. A preventive mechanism of green tea on obesity in C57BL/6 mice fed a high-fat (HF) diet was investigated by evaluating the expression levels of obesity-related proteins in mesenteric white adipose tissue by using protein array. An increase in the expression level of insulin-like growth factor binding protein (IGFBP)-1 by green tea was found in the white adipose tissues of both control and HF diet-fed mice by protein array and confirmed by Western blot. Moreover, the expression level was negatively correlated with adipose tissue weight. In 3T3-L1 adipocytes, treatment with green tea and its major polyphenol, (-)-epigallocatechin gallate, induced the expression of IGFBP-1 in a dose-dependent manner by Western blot. In conclusion, IGFBP-1 in adipose tissue is a novel molecule target for the prevention of obesity by green tea.
authors.affiliation: Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, Hyogo 657-8501, Japan.
authors.forename: Manabu
authors.initials: M
authors.lastname: Ueda
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Ashida
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D019440:Anti-Obesity Agents / Q000008:administration & dosage*; D059305:Diet, High-Fat / Q000009:adverse effects; D006801:Humans; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000235:genetics* / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000188:drug therapy / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D010936:Plant Extracts / Q000008:administration & dosage*; D013662:Tea / Q000737:chemistry*; D015854:Up-Regulation / Q000187:drug effects*
chemical_list: D019440:Anti-Obesity Agents; D018970:Insulin-Like Growth Factor Binding Protein 1; D010936:Plant Extracts; D013662:Tea