title: Submerged fermentation of the edible mushroom Pleurotus ostreatus in a batch stirred tank bioreactor as a promising alternative for the effective production of bioactive metabolites.
abstract: The aim of this study was to investigate the potential of the submerged fermentation procedure in the production of bioactive metabolites of the common edible mushroom Pleurotus ostreatus. The biomass of the mushroom strain was produced by submerged fermentation in a batch stirred tank bioreactor and extracted by solvents of increasing polarity. The dichloromethane and methanol extract were fractioned by different techniques including Adsorption Chromatography and Fast Centrifugal Partition Chromatography (FCPC). The structures of pure compounds were elucidated with 1D/2D NMR-spectroscopic analyses, and chemical correlations combined with GC/MS and LC/MS experiments. Nineteen metabolites (e.g., fatty acids, phenolic metabolites, nucleotides and alkaloids) were isolated. Beyond the production of known metabolites, we report herein the production also of trans-3,4-dihydro-3,4,8-trihydroxynapthalen-1(2H)-one, indolo-3-carboxylic acid, 3-formylpyrrole and 4-hydroxybenzoic acid, that have pharmaceutical interest and are isolated for the first time from Pleurotus strains. This work indicates the great potential of the established bioprocess for the production of P. ostreatus mycelia with enhanced metabolic profile.
authors.affiliation: BIOtechMASS Unit, Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 9 Iroon Polytechniou Street, Zografou Campus, GR-15700 Athens, Greece. fokialakis@pharm.uoa.gr
authors.forename: Lefki-Maria
authors.initials: LM
authors.lastname: Papaspyridi
authors.forename: Nektarios
authors.initials: N
authors.lastname: Aligiannis
authors.forename: Evangelos
authors.initials: E
authors.lastname: Topakas
authors.forename: Paul
authors.initials: P
authors.lastname: Christakopoulos
authors.forename: Alexandros-Leandros
authors.initials: AL
authors.lastname: Skaltsounis
authors.forename: Nikolas
authors.initials: N
authors.lastname: Fokialakis
mesh_terms: D061249:Batch Cell Culture Techniques; D001688:Biological Products / Q000302:isolation & purification / Q000378:metabolism*; D019149:Bioreactors; D005591:Chemical Fractionation; D002853:Chromatography, Liquid / Q000379:methods; D005227:Fatty Acids / Q000302:isolation & purification / Q000378:metabolism; D005285:Fermentation; D000432:Methanol / Q000737:chemistry; D008752:Methylene Chloride / Q000737:chemistry; D020076:Pleurotus / Q000254:growth & development* / Q000378:metabolism; D012997:Solvents / Q000737:chemistry
chemical_list: D001688:Biological Products; D005227:Fatty Acids; D012997:Solvents; D008752:Methylene Chloride; D000432:Methanol