title: Association of the physical and chemical properties and the cytotoxicity of metal oxide nanoparticles: metal ion release, adsorption ability and specific surface area.
abstract: Association of cellular influences and physical and chemical properties were examined for 24 kinds of industrial metal oxide nanoparticles: ZnO, CuO, NiO, Sb(2)O(3), CoO, MoO(3), Y(2)O(3), MgO, Gd(2)O(3), SnO(2), WO(3), ZrO(2), Fe(2)O(3), TiO(2), CeO(2), Al(2)O(3), Bi(2)O(3), La(2)O(3), ITO, and cobalt blue pigments. We prepared a stable medium dispersion for each nanoparticle and examined the influence on cell viability and oxidative stress together with physical and chemical characterizations. ZnO, CuO, NiO, MgO, and WO(3) showed a large amount of metal ion release in the culture medium. The cellular influences of these soluble nanoparticles were larger than insoluble nanoparticles. TiO(2), SnO(2), and CeO(2) nanoparticles showed strong protein adsorption ability; however, cellular influences of these nanoparticles were small. The primary particle size and the specific surface area seemed unrelated to cellular influences. Cellular influences of metal oxide nanoparticles depended on the kind and concentrations of released metals in the solution. For insoluble nanoparticles, the adsorption property was involved in cellular influences. The primary particle size and specific surface area of metal oxide nanoparticles did not affect directly cellular influences. In conclusion the most important cytotoxic factor of metal oxide nanoparticles was metal ion release.
authors.affiliation: Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Kitakyushu, Fukuoka 807-8555, Japan. masa-horie@med.uoeh-u-ac.jp
authors.forename: Masanori
authors.initials: M
authors.lastname: Horie
authors.forename: Katsuhide
authors.initials: K
authors.lastname: Fujita
authors.forename: Haruhisa
authors.initials: H
authors.lastname: Kato
authors.forename: Shigehisa
authors.initials: S
authors.lastname: Endoh
authors.forename: Keiko
authors.initials: K
authors.lastname: Nishio
authors.forename: Lilian Kaede
authors.initials: LK
authors.lastname: Komaba
authors.forename: Ayako
authors.initials: A
authors.lastname: Nakamura
authors.forename: Arisa
authors.initials: A
authors.lastname: Miyauchi
authors.forename: Shinichi
authors.initials: S
authors.lastname: Kinugasa
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Hagihara
authors.forename: Etsuo
authors.initials: E
authors.lastname: Niki
authors.forename: Yasukazu
authors.initials: Y
authors.lastname: Yoshida
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Iwahashi
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry*; D008670:Metals / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology; D010087:Oxides / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology; D010316:Particle Size; D017382:Reactive Oxygen Species / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism; D013499:Surface Properties
chemical_list: D008670:Metals; D010087:Oxides; D017382:Reactive Oxygen Species; D012710:Serum Albumin, Bovine