title: Metabolomic profiles of myocardial ischemia under treatment with salvianolic acid B.
abstract: BACKGROUND
  Radix Salvia miltiorrhiza (Danshen) has been used as a principal herb in treating cardiovascular diseases in Chinese medicine. Salvianolic acid B (SA-B), a water-soluble active component of Danshen, was found to have anti-myocardial ischemia (anti-MI) effect. This study aims to investigate mechanisms of SA-B on MI.
  
  
  METHODS
  Five conventional Western medicines (isosorbide dinitrate, verapamil, propranolol, captopril and trimethazine) with different mechanisms for treating cardiovascular diseases were selected as positive references to compare with SA-B in changing of the metabolomic profiles in MI rats under treatment. Potential mechanisms of SA-B were further investigated in H9C2 cell line.
  
  
  RESULTS
  The metabolomic profiles between SA-B- and propranolol-treated MI rats were similar, since there was a big overlap between the two groups in the PLS-DA score plot. Finally, it was demonstrated that SA-B exhibited a protective effect on MI mainly by decreasing the concentration of cyclic adenosine monophosphate (cAMP) and Ca2+ and inhibiting protein kinase A (PKA).
  
  
  CONCLUSIONS
  SA-B and propanolol exhibited similar metabolomic profiles, indicating that the two drugs might have a similar mechanism.
authors.affiliation: Department of Medicinal Chemistry of Nature Product, School of Pharmacy, Second Military Medical University, No, 325 Guohe Road, Shanghai 200433, China. ltj204@163.com.
authors.forename: Yonghai
authors.initials: Y
authors.lastname: Lu
authors.forename: Yue
authors.initials: Y
authors.lastname: Zheng
authors.forename: Xinru
authors.initials: X
authors.lastname: Liu
authors.forename: Xu
authors.initials: X
authors.lastname: Liang
authors.forename: Saiming
authors.initials: S
authors.lastname: Ngai
authors.forename: Tiejun
authors.initials: T
authors.lastname: Li
authors.forename: Weidong
authors.initials: W
authors.lastname: Zhang