title: Disposable immunochips for the detection of Legionella pneumophila using electrochemical impedance spectroscopy.
abstract: The rapid diagnosis of Legionellosis is crucial for the effective treatment of this disease. Currently, most clinical laboratories utilize rapid immunoassays that are sufficient for the detection of Legionella serogroup 1, but not other clinically relevant serogroups. In this report, the development of a disposable immunochip system is described in connection with electrochemical impedance spectroscopy and fluorescence microscopy. The immunochips were prepared by covalently immobilizing fluorophore-conjugated L. pneumophilaantibodies on Au chips. The analytical performance of the immunochips was optimized as a prescreening tool for L. pneumophila. The versatile immunochips described here can be easily adapted for the monitoring of all Legionella serogroups in clinical and environmental samples.
authors.forename: Nan
authors.initials: N
authors.lastname: Li
authors.forename: Arujun
authors.initials: A
authors.lastname: Brahmendra
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Veloso
authors.forename: Akriti
authors.initials: A
authors.lastname: Prashar
authors.forename: Xin R
authors.initials: XR
authors.lastname: Cheng
authors.forename: Vinci W S
authors.initials: VW
authors.lastname: Hung
authors.forename: Cyril
authors.initials: C
authors.lastname: Guyard
authors.forename: Mauricio
authors.initials: M
authors.lastname: Terebiznik
authors.forename: Kagan
authors.initials: K
authors.lastname: Kerman
mesh_terms: D058266:Dielectric Spectroscopy; D006801:Humans; D007120:Immunochemistry; D016952:Legionella pneumophila; D007876:Legionellosis / Q000175:diagnosis*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000639:trends; D008856:Microscopy, Fluorescence