title: Enhancement of gastric cell radiation sensitivity by chitosan-modified gold nanoparticles.
abstract: We explored the biocompatible gold nanoparticles (GNPs) with surface modified by chitosan in the applications of cell's response to X-ray irradiation. Substantial amounts of chitosan modified gold nanoparticles (CS-GNPs) were found to be internalized in cell cytoplasm revealed by transmission electron microscopy. Their in vitro cytotoxicity effects on MGC-803 cells and GES-1 cells were observed at 24, 48 and 72 h. The MTT results showed that CS-GNPs own excellent biocompatibility. The dose enhancement based on CS-GNPs induced the damage of MGC-803 cells under X-ray irradiation, monitored by clonogenic cell survival assay. We also investigated their effects on the survival rates of MGC-803 cells during irradiation for a dose up to 10 Gy using a radio-oncology linear accelerator (6 MeV). The survival fraction of cells incubated with different concentration of CS-GNPs was obviously reduced in comparison with that irradiation alone. The result also revealed an increase of cell inhibition with increasing the concentration of CS-GNPs. In conclusion, CS-GNPs can enhance the cell radiation therapeutic sensitivity, and have potential application in tumor irradiation therapy in near future.
authors.affiliation: National Key Laboratory of Nano/Micro Fabrication Technology, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Institute of Micro-Nano Science and Technology, Shanghai Jiao Tong University, Shanghai 200240, P. R. China.
authors.forename: Chunlei
authors.initials: C
authors.lastname: Zhang
authors.forename: Peng
authors.initials: P
authors.lastname: Huang
authors.forename: Le
authors.initials: L
authors.lastname: Bao
authors.forename: Meng
authors.initials: M
authors.lastname: He
authors.forename: Teng
authors.initials: T
authors.lastname: Luo
authors.forename: Guo
authors.initials: G
authors.lastname: Gao
authors.forename: Daxiang
authors.initials: D
authors.lastname: Cui
mesh_terms: D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology*; D005753:Gastric Mucosa / Q000166:cytology / Q000187:drug effects* / Q000528:radiation effects*; D006046:Gold / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D011838:Radiation-Sensitizing Agents / Q000737:chemistry / Q000494:pharmacology*; D013274:Stomach Neoplasms / Q000188:drug therapy / Q000532:radiotherapy; D014965:X-Rays
chemical_list: D011838:Radiation-Sensitizing Agents; D006046:Gold; D048271:Chitosan