title: A non-peptide receptor inhibitor with selectivity for one of the neutrophil formyl peptide receptors, FPR 1.
abstract: The neutrophil formyl peptide receptors (FPR1 and FPR2) are members of the G-protein coupled receptor family. The signals generated by occupied FPRs are both pro-inflammatory and anti-inflammatory. Accordingly, these receptors have become a therapeutic target for the development of novel drugs that may be used to reduce injuries in inflammatory diseases including asthma, rheumatoid arthritis, Alzheimer's disease and cardiovascular diseases. To support the basis for a future pharmacological characterization, we have identified a small molecular non-peptide inhibitor with selectivity for FPR1. We used the FPR1 and FPR2 specific ligands fMLF and WKYMVM, respectively, and an earlier described ratio technique, to determine inhibitory activity combined with selectivity. We show that the compound 3,5-dichloro-N-(2-chloro-5-methyl-phenyl)-2-hydroxy-benzamide (BVT173187) fulfills the criteria for an FPR1 inhibitor selective for FPR1 over FPR2, and it inhibits the same functional repertoire in neutrophils as earlier described peptide antagonists. Accordingly, the new inhibitor reduced neutrophil activation with FPR1 agonists, leading to mobilization of adhesion molecules (CR3) and the generation of superoxide anion from the neutrophil NADPH-oxidase. The effects of a number of structural analogs were determined but these were either without activity or less active/specific than BVT173187. The potency of the new inhibitor for reduction of FPR1 activity was the same as that of the earlier described FPR1 antagonist cyclosporine H, but signaling through the C5aR and CXCR (recognizing IL8) was also affected by BVT173187.
authors.affiliation: Department of Rheumatology and Inflammation Research, Institute of Medicine, University of Gothenburg, Box 480, S-405 30 Göteborg, Sweden.
authors.forename: Hülya
authors.initials: H
authors.lastname: Cevik-Aras
authors.forename: Christina
authors.initials: C
authors.lastname: Kalderén
authors.forename: Annika
authors.initials: A
authors.lastname: Jenmalm Jensen
authors.forename: Tudor
authors.initials: T
authors.lastname: Oprea
authors.forename: Claes
authors.initials: C
authors.lastname: Dahlgren
authors.forename: Huamei
authors.initials: H
authors.lastname: Forsman
mesh_terms: D001549:Benzamides / Q000737:chemistry / Q000494:pharmacology*; D002478:Cells, Cultured; D005434:Flow Cytometry; D006801:Humans; D008024:Ligands; D009504:Neutrophils / Q000378:metabolism*; D044042:Receptors, Formyl Peptide / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: C572914:3,5-dichloro-N-(2-chloro-5-methylphenyl)-2-hydroxybenzamide; D001549:Benzamides; C469986:FPR1 protein, human; D008024:Ligands; D044042:Receptors, Formyl Peptide