title: [Secondary autoimmune hemolytic anemia as a result of B19 parvovirus persistence in immunodeficient patients].
abstract: OBJECTIVE
  To ascertain informative value of immunological diagnosis of B19 parvovirus in combination with polymerase chain reaction (PCR); to analyse frequency of development of secondary autoimmune hemolytic anemia (AIHA) in immunodeficient patients as a result of virus persistence--persistent infection eliminated only by treatment causing suppression of erythropoiesis.
  
  
  METHODS
  B19 parvovirus detection was performed in blood serum of 207 subjects: 144 patients with anemia (Hb < 100 g/1) and 500 blood donors. DNA of parvovirus B19 was detected in the sera by PCR, antibodies to this virus--by enzyme immunoassay (EIA). IgG, IgM, IgA and components of compliment Clq, C3 on the surface of erythrocytes were detected by EIA in anemic patients.
  
  
  RESULTS
  Parvovirus infection was registered in 30% patients, in 70% the infection was persistent. The latter were diagnosed to have secondary AIHA.
  
  
  CONCLUSIONS
  Combined application of PCR and EIA extends potentialities of diagnosis of infection caused by B19 parvovirus. Persistence of the parvovirus provokes onset of symptomatic hemolytic anemia in immunodeficient patients.
authors.forename: O E
authors.initials: OE
authors.lastname: Iaguzhinskaia
authors.forename: I S
authors.initials: IS
authors.lastname: Fevraleva
authors.forename: M A
authors.initials: MA
authors.lastname: Elizhbaeva
authors.forename: A A
authors.initials: AA
authors.lastname: Levina
authors.forename: G M
authors.initials: GM
authors.lastname: Semenova
authors.forename: N Iu
authors.initials: NIu
authors.lastname: Bugar'
authors.forename: L M
authors.initials: LM
authors.lastname: Tsybul'skaia
authors.forename: E Iu
authors.initials: EIu
authors.lastname: Varlamova
authors.forename: I V
authors.initials: IV
authors.lastname: Shitareva
authors.forename: N I
authors.initials: NI
authors.lastname: Tsyba
authors.forename: S K
authors.initials: SK
authors.lastname: Kravchenko
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000744:Anemia, Hemolytic, Autoimmune / Q000097:blood / Q000209:etiology* / Q000276:immunology; D001323:Autoantibodies / Q000097:blood; D002648:Child; D002675:Child, Preschool; D006801:Humans; D016867:Immunocompromised Host; D007124:Immunoenzyme Techniques; D007136:Immunoglobulins / Q000097:blood; D007223:Infant; D008875:Middle Aged; D010322:Parvoviridae Infections / Q000097:blood / Q000276:immunology* / Q000821:virology; D016732:Parvovirus B19, Human / Q000276:immunology* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D055815:Young Adult
chemical_list: D001323:Autoantibodies; D007136:Immunoglobulins