title: Mitochondrial genome of Onychostoma lini (Teleostei, Cypriniformes).
abstract: The complete mitogenome sequence of a vulnerable species Onychostoma lini was determined using polymerase chain reaction and directly sequenced with primer walking method. The complete mitogenome was 16,595 bp in length, containing 37 genes of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and a control region (D-loop). The gene order and composition of O. lini was similar to most other fishes. The descending order of the base composition on heavy strand was 31.6% A, 27.9% C, 24.6% T, and 15.9% G, with a relatively lower level of G and a slight AT bias of 56.2%. The codon usage followed the typical vertebrate mitochondrial pattern (ATG or GTG for start codon and TAA or TAG for stop codon). The complete mitogenome sequence of O. lini provides fundamental data for further conversation genetics and captive breeding studies on this species.
authors.affiliation: The Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Science, Southwest University, Beibei, Chongqing, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Huang
authors.forename: Guoqing
authors.initials: G
authors.lastname: Zhao
authors.forename: Zuogang
authors.initials: Z
authors.lastname: Peng
mesh_terms: D000818:Animals; D001482:Base Composition; D002681:China; D003062:Codon; D003531:Cypriniformes / Q000145:classification / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics; D023061:Gene Order; D020459:Genes, rRNA; D054629:Genome, Mitochondrial / Q000235:genetics*; D024101:Mitochondrial Proteins / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D012343:RNA, Transfer / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D003062:Codon; D004272:DNA, Mitochondrial; D024101:Mitochondrial Proteins; D012343:RNA, Transfer