title: Dynamic regulation of sarcoplasmic reticulum Ca(2+)  stores by stromal interaction molecule 1 and sarcolipin during muscle differentiation.
abstract: During muscle development, the sarco/endoplasmic reticulum (SR/ER) undergoes remodeling to establish a specialized internal Ca(2+) store for muscle contraction. We hypothesized that store operated Ca(2+) entry (SOCE) is required to fill Ca(2+) stores and is, therefore, critical to creating a mature SR/ER. Stromal interaction molecule 1 (STIM1) functions as a sensor of internal Ca(2+) store content and an activator of SOCE channels. Myocytes lacking STIM1 display reduced SR Ca(2+) content and altered expression of key SR proteins. Sarcolipin (SLN), an inhibitor of the SR calcium pump, was markedly increased in the muscle of mutant STIM1 mice. SLN opposes the actions of STIM1 by limiting SOCE, reducing SR Ca(2+) content and delaying muscle differentiation. During mouse muscle development SLN is highly expressed in embryonic muscle, while the expression of STIM1 is up-regulated postnatally. These results suggest that SOCE regulates SR/ER specialization and that SLN and STIM1 act in opposing fashions to govern SOCE during myogenesis.
authors.affiliation: Department of Medicine, Duke University, Durham, North Carolina 27710, USA.
authors.forename: Malini
authors.initials: M
authors.lastname: Seth
authors.forename: Tianyu
authors.initials: T
authors.lastname: Li
authors.forename: Victoria
authors.initials: V
authors.lastname: Graham
authors.forename: Jarrett
authors.initials: J
authors.lastname: Burch
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Finch
authors.forename: Jonathan A
authors.initials: JA
authors.lastname: Stiber
authors.forename: Paul B
authors.initials: PB
authors.lastname: Rosenberg
mesh_terms: D000818:Animals; D002118:Calcium / Q000502:physiology*; D015220:Calcium Channels; D020013:Calcium Signaling; D002454:Cell Differentiation; D002478:Cells, Cultured; D004721:Endoplasmic Reticulum / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D008562:Membrane Glycoproteins / Q000502:physiology*; D051379:Mice; D024510:Muscle Development; D009124:Muscle Proteins / Q000502:physiology*; D018482:Muscle, Skeletal / Q000166:cytology / Q000502:physiology; D011510:Proteolipids / Q000502:physiology*; D000071737:Stromal Interaction Molecule 1
chemical_list: D015220:Calcium Channels; D008562:Membrane Glycoproteins; D009124:Muscle Proteins; D011510:Proteolipids; C100757:Stim1 protein, mouse; D000071737:Stromal Interaction Molecule 1; C077258:sarcolipin; D002118:Calcium