title: Inhibitory effect of cisplatin and [Pt(dach)Cl2] on the activity of phospholipase A2.
abstract: This work has been focused on testing the influence of two selected Pt(II) complexes cisplatin, Pt(NH₃)₂Cl₂, and [Pt(dach)Cl₂] on the activity of porcine pancreatic phospholipase A₂ (PLA₂). It has been assumed that this enzyme plays a role in carcinogenesis and that it could be a target in the tumour therapy. The results of this study show that both Pt(II) complexes inhibit the activity of the enzyme, though they bind to it in a different manner. While cisplatin interacts with the enzyme in an acompetitive manner, the stable interaction of [Pt(dach)Cl₂] with PLA₂ could not be detected under our experimental conditions.
authors.affiliation: Laboratory of Physical Chemistry, Vinča Institute of Nuclear Sciences, University of Belgrade, Belgrade, Republic of Serbia.
authors.forename: Maja
authors.initials: M
authors.lastname: Radisavljević
authors.forename: Tina
authors.initials: T
authors.lastname: Kamčeva
authors.forename: Živadin D
authors.initials: ŽD
authors.lastname: Bugarčić
authors.forename: Marijana
authors.initials: M
authors.lastname: Petković
mesh_terms: D055162:Biocatalysis; D002945:Cisplatin / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D009944:Organoplatinum Compounds / Q000737:chemistry / Q000494:pharmacology*; D064801:Phospholipase A2 Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D054467:Phospholipases A2 / Q000737:chemistry / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013329:Structure-Activity Relationship
chemical_list: D009944:Organoplatinum Compounds; D064801:Phospholipase A2 Inhibitors; C013933:dichloro-1,2-diaminocyclohexane platinum complex; D054467:Phospholipases A2; D002945:Cisplatin