title: Cadmium ion-doped magnetic poly(styrene-acrylic acid) nanospheres for sensitive electrochemical immunoassay.
abstract: A novel class of molecular tags, cadmium ion-doped magnetic poly(styrene-acrylic acid) nanospheres (Cd-MPSA), was first synthesized and functionalized with polyclonal rabbit anti-human luteinizing hormone antibodies (PAb(2)) for highly efficient electrochemical immunoassay of luteinizing hormone (LH). Transmission electron microscope (TEM) and Fourier transform infrared spectroscope (FTIR) were employed to characterize the prepared Cd-MPSA. By using Cd-MPSA-labeled PAb(2) as molecular tags, a novel sandwich-type immunoassay protocol was built for determination of LH on monoclonal mouse anti-human luteinizing hormone antibody (MAb(1))-functionalized gold electrode. The assay was carried out in pH 5.3 HAc-NaAc buffer solution by square wave voltammetry (SWV). The signal was obtained by the reduction of the doped cadmium ions in the Cd-MPSA. Under optimal conditions, the currents increased with the increasing LH level in the sample, and exhibited a linear range from 0.25 to 240 mIU mL(-1) with a detection limit of 0.08 mIU mL(-1) LH at 3s(B). The precision, reproducibility, and specificity were acceptable. No obvious difference was encountered in the analysis of spiking LH samples into newborn calf serum with the referenced values.
authors.affiliation: Key Laboratory of Analysis and Detection for Food Safety (Ministry of Education & Fujian Province), Department of Chemistry, Fuzhou University, Fuzhou 350108, PR China.
authors.forename: Bing
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Analysis and Detection for Food Safety (Ministry of Education & Fujian Province), Department of Chemistry, Fuzhou University, Fuzhou 350108, PR China.
authors.forename: Yuling
authors.initials: Y
authors.lastname: Cui
authors.affiliation: Key Laboratory of Analysis and Detection for Food Safety (Ministry of Education & Fujian Province), Department of Chemistry, Fuzhou University, Fuzhou 350108, PR China.
authors.forename: Bingqian
authors.initials: B
authors.lastname: Liu
authors.affiliation: Key Laboratory of Analysis and Detection for Food Safety (Ministry of Education & Fujian Province), Department of Chemistry, Fuzhou University, Fuzhou 350108, PR China.
authors.forename: Huafeng
authors.initials: H
authors.lastname: Chen
authors.affiliation: Key Laboratory of Analysis and Detection for Food Safety (Ministry of Education & Fujian Province), Department of Chemistry, Fuzhou University, Fuzhou 350108, PR China.
authors.forename: Guonan
authors.initials: G
authors.lastname: Chen
authors.affiliation: Key Laboratory of Analysis and Detection for Food Safety (Ministry of Education & Fujian Province), Department of Chemistry, Fuzhou University, Fuzhou 350108, PR China. Electronic address: dianping.tang@fzu.edu.cn.
authors.forename: Dianping
authors.initials: D
authors.lastname: Tang
mesh_terms: D000179:Acrylates; D000818:Animals; D055910:Antibodies, Immobilized; D058846:Antibodies, Monoclonal, Murine-Derived; D015374:Biosensing Techniques / Q000379:methods* / Q000706:statistics & numerical data; D002104:Cadmium; D002417:Cattle; D055664:Electrochemical Techniques; D006801:Humans; D007118:Immunoassay / Q000379:methods* / Q000706:statistics & numerical data; D057230:Limit of Detection; D007986:Luteinizing Hormone / Q000032:analysis / Q000097:blood / Q000276:immunology; D008280:Magnetics; D051379:Mice; D046529:Microscopy, Electron, Transmission; D054118:Nanospheres / Q000737:chemistry / Q000648:ultrastructure; D011137:Polystyrenes; D011817:Rabbits; D012680:Sensitivity and Specificity; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000179:Acrylates; D055910:Antibodies, Immobilized; D058846:Antibodies, Monoclonal, Murine-Derived; D011137:Polystyrenes; C509855:poly(styrene-b-acrylic acid); D002104:Cadmium; D007986:Luteinizing Hormone