title: Sequence and phylogenetic data indicate that an orthobunyavirus recently detected in the Yucatan Peninsula of Mexico is a novel reassortant of Potosi and Cache Valley viruses.
abstract: We determined the complete nucleotide sequences of the small (S) and medium (M) RNA segments of an orthobunyavirus isolated from mosquitoes in the Yucatan Peninsula of Mexico. A 528-nt region of the large (L) RNA segment was also sequenced. The S RNA segment has greatest nucleotide identity to the homologous region of Cache Valley virus (CVV; 98%) followed by Potosi virus (POTV; 89%) and Northway virus (86%). The M RNA segment has 96% nucleotide identity to the homologous region of POTV, and less than 74% nucleotide identity to the homologous regions of all other orthobunyaviruses for which M segment sequence data are available. The L RNA segment has greatest nucleotide identity to the homologous region of POTV (98%) followed by CVV (82%) and Tensaw virus (77%). These data indicate that the virus, tentatively named Cholul virus (CHLV), is a novel reassortant that acquired its S RNA segment from CVV and its M and L RNA segments from POTV. Phylogenetic data support this conclusion.
authors.affiliation: Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, 50011, USA. blitvich@iastate.edu
authors.forename: Bradley J
authors.initials: BJ
authors.lastname: Blitvich
authors.forename: Rungrat
authors.initials: R
authors.lastname: Saiyasombat
authors.forename: Karin S
authors.initials: KS
authors.lastname: Dorman
authors.forename: Julian E
authors.initials: JE
authors.lastname: Garcia-Rejon
authors.forename: Jose A
authors.initials: JA
authors.lastname: Farfan-Ale
authors.forename: Maria A
authors.initials: MA
authors.lastname: Loroño-Pino
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002042:Bunyamwera virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification*; D009033:Culicidae / Q000821:virology; D008800:Mexico; D008969:Molecular Sequence Data; D010802:Phylogeny; D016865:Reassortant Viruses / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D011995:Recombination, Genetic; D017385:Sequence Homology; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins