title: A polymer-based functional pattern on one-dimensional photonic crystals for photon sorting of fluorescence radiation.
abstract: In this work we introduce the use of a patterned polymer-based surface functionalization of a one-dimensional photonic crystal (1DPC) for controlling the emission direction of fluorescent proteins (ptA) via coupling to a set of two Bloch Surface Waves (BSW). Each BSW dispersion branch relates to a micrometric region on the patterned 1DPC, characterized by a well defined chemical characteristic. We report on the enhanced and spatially selective excitation of fluorescent ptA, and on the spatially-resolved detection of polarized emitted radiation coupled to specific BSW modes. As a result, we provide an optical multiplexing technique for the angular separation of fluorescence radiated from micrometric regions having different surface properties, even in the case the emitting labels are spectrally identical. This working principle can be advantageously extended to a multi-step nanometric relief structure for self-referencing biosensing or frequency-multiplexed fluorescence detection.
authors.affiliation: Department of Applied Sciences and Technology, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Torino, Italy.
authors.forename: Mirko
authors.initials: M
authors.lastname: Ballarini
authors.forename: Francesca
authors.initials: F
authors.lastname: Frascella
authors.forename: Natascia
authors.initials: N
authors.lastname: De Leo
authors.forename: Serena
authors.initials: S
authors.lastname: Ricciardi
authors.forename: Paola
authors.initials: P
authors.lastname: Rivolo
authors.forename: Pietro
authors.initials: P
authors.lastname: Mandracci
authors.forename: Emanuele
authors.initials: E
authors.lastname: Enrico
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Giorgis
authors.forename: Francesco
authors.initials: F
authors.lastname: Michelotti
authors.forename: Emiliano
authors.initials: E
authors.lastname: Descrovi
mesh_terms: D005455:Fluorescent Antibody Technique / Q000379:methods*; D008422:Materials Testing; D054802:Molecular Imprinting / Q000379:methods*; D017785:Photons; D011108:Polymers / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D020349:Surface Plasmon Resonance / Q000379:methods*; D013499:Surface Properties
chemical_list: D011108:Polymers