title: Effect of TGF-β on Gli2 expression in HL60 and U937 cell lines.
abstract: The Hedgehog (Hh) signaling pathway is involved in a variety of tumor-related diseases, including leukemia. The aim of this study was to determine whether there is an interaction between the Hh signaling pathway and transforming growth factor-β (TGF-β) in the HL60 and U937 cell lines. HL60 and U937 cells were treated with TGF-β, tumor necrosis factor-α (TNF-α) and specific inhibitor of Smad3 (SIS3). The expression of Gli2 was detected by real-time PCR and western blotting. The results showed that TGF-β alone did not affect the Gli2 expression in HL60 cells, while it significantly reduced the level of Gli2 expression in U937 cells. TGF-β+TNF-α reduced the expression of Gli2 in HL60 cells and U937 cells. The reduction in the level of Gli2 expression in U937 cells by TGF-β+TNF-α was greater than that caused by TGF-β alone. SIS3 inhibited the effect of TGF-β. Our results suggest that the effect on Gli2 expression in HL60 and U937 cells induced by TGF-β is Smad3-dependent and independent of Hh receptor signaling.
authors.affiliation: Department of Hematology, The First Affiliated Hospital, China Medical University, Shenyang, Liaoning 110001, PR China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Li
authors.forename: Deng
authors.initials: D
authors.lastname: Pan
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
mesh_terms: D005786:Gene Expression Regulation / Q000187:drug effects*; D018922:HL-60 Cells; D053823:Hedgehog Proteins / Q000378:metabolism; D006801:Humans; D051741:Kruppel-Like Transcription Factors / Q000096:biosynthesis*; D009687:Nuclear Proteins / Q000096:biosynthesis*; D015398:Signal Transduction / Q000187:drug effects*; D051900:Smad3 Protein / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D020298:U937 Cells; D000075746:Zinc Finger Protein Gli2
chemical_list: C082593:GLI2 protein, human; D053823:Hedgehog Proteins; D051741:Kruppel-Like Transcription Factors; D009687:Nuclear Proteins; D051900:Smad3 Protein; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha; D000075746:Zinc Finger Protein Gli2