title: Preparation and initial application of a monoclonal antibody specific for a newly discovered conserved linear epitope of rabies virus nucleoprotein.
abstract: OBJECTIVE
  To prepare monoclonal antibodies against a newly discovered and conserved linear epitope of Rabies virus nucleoprotein and to use them in a rabies diagnostic test.
  
  
  METHODS
  Synthetic peptide containing the epitope was used as immunogen to prepare hybridoma cell lines by classical hybridoma technology. Anti-peptide monoclonal antibodies produced in ascites of inoculated Balb/c mice were labeled with fluorescein isothiocyanate (FITC) after purification and used in fluorescent antibody test (FAT).
  
  
  RESULTS
  Two positive hybridoma cell lines, RVNP-mAb1-CL and RVNP-mAb2-CL, were obtained. RVNP- mAb1-CL produced a higher concentration of monoclonal antibody RVNP-mAb1 in Balb/c ascites. FITC-labeled RVNP-mAb1 showed correct results on certain Rabies virus-positive canine brain tissue samples and cells of a small subclone of baby hamster kidney 21 cell line (BSR).
  
  
  CONCLUSIONS
  FITC-labeled RVNP-mAb1 has potential application for laboratory diagnosis of rabies.
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, Department of Viral Encephalitis, Institute for Viral Disease Control and Prevention, Chinese Centers for Disease Control and Prevention, Beijing, China.
authors.forename: Xin Jun
authors.initials: XJ
authors.lastname: Lv
authors.forename: Xue Jun
authors.initials: XJ
authors.lastname: Ma
authors.forename: Li Hua
authors.initials: LH
authors.lastname: Wang
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.forename: Xin Xin
authors.initials: XX
authors.lastname: Shen
authors.forename: Peng Cheng
authors.initials: PC
authors.lastname: Yu
authors.forename: Qing
authors.initials: Q
authors.lastname: Tang
authors.forename: Guo Dong
authors.initials: GD
authors.lastname: Liang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D002460:Cell Line; D006224:Cricetinae; D004285:Dogs; D000939:Epitopes; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C; D009698:Nucleoproteins / Q000276:immunology*; D011820:Rabies virus / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D005456:Fluorescent Dyes; D009698:Nucleoproteins; D014764:Viral Proteins; D016650:Fluorescein-5-isothiocyanate