title: The effects of epidermal growth factor and transforming growth factor-α on secretion of interleukin-8 and growth-regulated oncogene-α in human granulosa-lutein cells.
abstract: BACKGROUND
  In order to investigate the roles of epidermal growth factor (EGF) and transforming growth factor (TGF)-α in ovulation, we studied the production of interleukin (IL)-8 and growth-regulated oncogene (GRO)-α in cultured human granulosa-lutein cells.
  
  
  METHODS
  Granulosa-lutein cells obtained from the follicular fluids of in vitro fertilization and embryo transfer patients were cultured and treated with EGF, TGF-α, tumor necrosis factor (TNF)-α or 12-O-tetradecanoylphorbol 13-acetate (TPA). An immortalized granulosa cell line (GC1a) was also cultured and treated with EGF, TGF-α or mitogen-activated protein kinase kinase inhibitor. The supernatants were collected, and IL-8 and GRO-α were measured by ELISA.
  
  
  RESULTS
  The levels of IL-8 and GRO-α were significantly increased after treatment with EGF, TGF-α, TNF-α and TPA by primary cultured granulosa-lutein cells. The levels of IL-8 and GRO-α were also significantly increased after treatment with EGF or TGF-α in a dose-dependent manner by GC1a. When GC1a was treated with EGF, TGF-α or U0126, the levels of IL-8 and GRO-α were significantly decreased.
  
  
  CONCLUSIONS
  Our data indicate that the production of IL-8 and GRO-α is upregulated by EGF and TGF-α. It is suggested that EGF and TGF-α may play an important role in luteinization processes involving IL-8 and GRO-α production.
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, Oita University, Oita, Japan. kawayas@oita-u.ac.jp
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Kawano
authors.forename: Kosay
authors.initials: K
authors.lastname: Zeineh
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Furukawa
authors.forename: Yufuko
authors.initials: Y
authors.lastname: Utsunomiya
authors.forename: Mamiko
authors.initials: M
authors.lastname: Okamoto
authors.forename: Hisashi
authors.initials: H
authors.lastname: Narahara
mesh_terms: D000328:Adult; D002460:Cell Line; D002478:Cells, Cultured; D054360:Chemokine CXCL1 / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005260:Female; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism*; D008184:Luteal Cells / Q000187:drug effects* / Q000378:metabolism; D016211:Transforming Growth Factor alpha / Q000494:pharmacology*; D015854:Up-Regulation; D055815:Young Adult
chemical_list: D054360:Chemokine CXCL1; D016209:Interleukin-8; D016211:Transforming Growth Factor alpha; D004815:Epidermal Growth Factor