title: Characterization and comparative analysis of small RNAs in three small RNA libraries of the brown planthopper (Nilaparvata lugens).
abstract: BACKGROUND
  The brown planthopper (BPH), Nilaparvata lugens (Stå;l), which belongs to Homopteran, Delphacidae, is one of the most serious and destructive pests of rice. Feeding BPH with homologous dsRNA in vitro can lead to the death of BPH, which gives a valuable clue to the prevention and control of this pest, however, we know little about its small RNA world.
  
  
  RESULTS
  Small RNA libraries for three developmental stages of BPH (CX-male adult, CC-female adult, CY-last instar female nymph) had been constructed and sequenced. It revealed a prolific small RNA world of BPH. We obtained a final list of 452 (CX), 430 (CC), and 381 (CY) conserved microRNAs (miRNAs), respectively, as well as a total of 71 new miRNAs in the three libraries. All the miRNAs had their own expression profiles in the three libraries. The phylogenic evolution of the miRNA families in BPH was consistent with other species. The new miRNA sequences demonstrated some base biases.
  
  
  CONCLUSIONS
  Our study discovered a large number of small RNAs through deep sequencing of three small RNA libraries of BPH. Many animal-conserved miRNA families as well as some novel miRNAs have been detected in our libraries. This is the first achievement to discover the small RNA world of BPH. A lot of new valuable information about BPH small RNAs has been revealed which was helpful for studying insect molecular biology and insect resistant research.
authors.affiliation: National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan, China.
authors.forename: Qiuhong
authors.initials: Q
authors.lastname: Chen
authors.forename: Lin
authors.initials: L
authors.lastname: Lu
authors.forename: Hongxia
authors.initials: H
authors.lastname: Hua
authors.forename: Fei
authors.initials: F
authors.lastname: Zhou
authors.forename: Liaoxun
authors.initials: L
authors.lastname: Lu
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Lin
mesh_terms: D000818:Animals; D001482:Base Composition; D016000:Cluster Analysis; D017124:Conserved Sequence; D019143:Evolution, Molecular; D005260:Female; D020869:Gene Expression Profiling; D015723:Gene Library; D049750:Genome, Insect; D006430:Hemiptera / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing; D008297:Male; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics*; D058977:Molecular Sequence Annotation; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D059370:RNA Folding; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D035683:MicroRNAs