title: Azumiobodo hoyamushi gen. nov. et sp. nov. (Euglenozoa, Kinetoplastea, Neobodonida): a pathogenic kinetoplastid causing the soft tunic syndrome in ascidian aquaculture.
abstract: We used morphological and genetic analyses to investigate a pathogenic kinetoplastid isolated from a diseased edible ascidian Halocynthia roretzi with soft tunic syndrome. The morphological characteristics of the kinetoplastid are similar to those in the order Neobodonida in the subclass Metakinetoplastida. However, the presence of unique globular bodies distinguishes this kinetoplastid from the other polykinetoplastic genera (i.e. Cruzella, Dimastigella and Rhynchobodo) in this order. These globular bodies are cytoplasmic inclusions without an outer delimiting membrane and are composed of a homologous granular matrix containing electron-dense bands. A phylogenetic tree based on 18S rRNA gene sequences also indicated that the kinetoplastid belongs to the order Neobodonida, although it forms an independent clade in this order. From these results, we propose a new genus in the order Neobodonida, i.e. Azumiobodo gen. nov., and Azumiobodo hoyamushi as the type species for the genus.
authors.affiliation: Department of Chemistry, Biology and Marine Science, University of the Ryukyus, Nishihara, Okinawa 903-0213, Japan.
authors.forename: Euichi
authors.initials: E
authors.lastname: Hirose
authors.forename: Akino
authors.initials: A
authors.lastname: Nozawa
authors.forename: Akira
authors.initials: A
authors.lastname: Kumagai
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Kitamura
mesh_terms: D000818:Animals; D017756:Aquaculture; D056898:Euglenozoa / Q000302:isolation & purification*; D010802:Phylogeny; D014561:Urochordata / Q000235:genetics / Q000469:parasitology* / Q000648:ultrastructure