title: Application of step-wise gradient high-performance counter-current chromatography for rapid preparative separation and purification of diterpene components from Pseudolarix kaempferi Gordon.
abstract: In general, simultaneously separation and purification of components with a broad polarity range from traditional Chinese medicine (TCM) is a challenge by an ordinary high-speed counter-current chromatography (HSCCC) method. In this paper, we describes a rapid and efficient separation method of combining three-step gradient elution and two-step flow-rate gradient elution using high-performance counter-current chromatography (HPCCC) to separate 8 diterpene compounds simultaneously within 80 min in a single run from the alcohol extract of Pseudolarix kaempferi Gordon. This separation process produced 166 mg pseudolaric acid B O-β-d-glucopyranoside (PABGly), 152 mg pseudolaric acid C (PAC), 8 mg deacetylpseudolaric acid A (deacetylPAA), 5 mg pseudolaric acid A O-β-d-glucopyranoside (PAAGly), 484 mg pseudolaric acid B (PAB), 33 mg pseudolaric acid B methyl ester (PAB methyl ester), 10mg pseudolaric acid A (PAA) and 18 mg pseudolaric acid H (PAH) from 1.0 g crude sample with purities of 98.6%, 99.6%, 92.3%, 92.2%, 99.2%, 99.4%, 98.3%, 91.0%, respectively. Our study indicates that the suitable combination of step-wise gradient elution and flow-rate gradient elution using HPCCC is an effective strategy to separate complex components from natural products.
authors.affiliation: State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, China.
authors.forename: Shichao
authors.initials: S
authors.lastname: He
authors.forename: Shucai
authors.initials: S
authors.lastname: Li
authors.forename: Jianhong
authors.initials: J
authors.lastname: Yang
authors.forename: Haoyu
authors.initials: H
authors.lastname: Ye
authors.forename: Shijie
authors.initials: S
authors.lastname: Zhong
authors.forename: Hang
authors.initials: H
authors.lastname: Song
authors.forename: Yongkui
authors.initials: Y
authors.lastname: Zhang
authors.forename: Cheng
authors.initials: C
authors.lastname: Peng
authors.forename: Aihua
authors.initials: A
authors.lastname: Peng
authors.forename: Lijuan
authors.initials: L
authors.lastname: Chen
mesh_terms: D003377:Countercurrent Distribution / Q000191:economics / Q000379:methods*; D004224:Diterpenes / Q000302:isolation & purification*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D028201:Pinaceae / Q000737:chemistry*; D013997:Time Factors
chemical_list: D004224:Diterpenes; D004365:Drugs, Chinese Herbal