title: An investigation of oxidative DNA damage in pharmacy technicians exposed to antineoplastic drugs in two Chinese hospitals using the urinary 8-OHdG assay.
abstract: OBJECTIVE
  To investigate oxidative DNA damage in pharmacy technicians preparing antineoplastic drugs at the PIVAS (Pharmacy Intravenous Admixture Service) in two Chinese hospitals.
  
  
  METHODS
  Urinary 8-OHdG served as a biomarker. 5-Fluorouracil (5-FU) concentrations in air, masks and gloves were determined. The spill exposure of each PIVAS technician to antineoplastic drugs was investigated. Eighty subjects were divided into exposed group I, II, and control group I, II.
  
  
  RESULTS
  5-FU concentration ratios for gloves and masks in exposed group I were significantly higher than those in exposed group II (P<0.05 or P<0.01). The average urinary 8-OHdG concentrations in exposed group I, control group I, exposed group II, and control group II were 14.69±0.93, 10.68±1.07, 10.57±0.55, and 11.96±0.73 ng/mg Cr, respectively. Urinary 8-OHdG concentration in exposed group I was significantly higher than that in control group I or that in exposed group II (P<0.01). There was a significant correlation between urinary 8-OHdG concentrations and spill frequencies per technician (P<0.01).
  
  
  CONCLUSIONS
  There was detectable oxidative DNA damage in PIVAS technicians exposed to antineoplastic drugs. This oxidative DNA damage may be associated with their spill exposure experience and contamination of their personal protective equipment.
authors.affiliation: The Second Affiliated Hospital of College of Medicine of Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Yu Wen
authors.initials: YW
authors.lastname: Huang
authors.forename: Le
authors.initials: L
authors.lastname: Jian
authors.forename: Mei Bian
authors.initials: MB
authors.lastname: Zhang
authors.forename: Quan
authors.initials: Q
authors.lastname: Zhou
authors.forename: Xiao Feng
authors.initials: XF
authors.lastname: Yan
authors.forename: Xu Dong
authors.initials: XD
authors.lastname: Hua
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhou
authors.forename: Ji Liang
authors.initials: JL
authors.lastname: He
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000388:Air / Q000032:analysis; D000970:Antineoplastic Agents / Q000032:analysis / Q000633:toxicity*; D016022:Case-Control Studies; D002681:China; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D005260:Female; D005472:Fluorouracil / Q000032:analysis / Q000633:toxicity*; D017575:Gloves, Protective; D006282:Health Personnel / Q000706:statistics & numerical data; D006761:Hospitals / Q000706:statistics & numerical data; D006801:Humans; D008297:Male; D008397:Masks; D016273:Occupational Exposure / Q000706:statistics & numerical data*; D018384:Oxidative Stress; D055815:Young Adult
chemical_list: D000970:Antineoplastic Agents; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine; D005472:Fluorouracil