title: Directly suspended droplet microextraction coupled with high performance liquid chromatography: a rapid and sensitive method for acetaldehyde assay in peritoneal dialysis fluids.
abstract: The aim of present study was to develop and validate a rapid, sensitive, inexpensive and reliable method for the detection of trace levels of acetaldehyde in peritoneal dialysis fluids (PDFs) by 2,4-dinitrophenylhydrazine (DNPH) derivatization and extraction. Separation and analysis of acetaldehyde via 2,4-dinitrophenylhydrazone (DNPH) was by reverse phase high performance liquid chromatography (HPLC). In order to remove co-eluting interferences and to pre-concentrate acetaldehyde, the extraction and clean-up of the sample has been performed using a liquid phase microextraction technique. In this research directly suspended droplet microextraction technique (DSDME) coupled with HPLC was used to determine acetaldehyde in PDFs. In DSDME method a free suspended droplet of an organic solvent (1-octanol) used as extraction phase. Important factors such as organic solvent, extraction time, droplet volume, sample and reagent solution volumes and rate of stirring were optimized. After extraction under optimal conditions the samples were analyzed by HPLC with UV detection at 360 nm. The linearity ranged from 0.01 to 100 mg L⁻¹ with a relative standard deviation (RSD%; n=3) 5.6 Enrichment factor and limit of detection (LOD; n=5) were 54 and 1.12 μg L⁻¹, respectively.
authors.affiliation: Department of Chemistry, Payame Noor University, 19395-4697 Tehran, Iran. eshaghi@pnu.ac.ir
authors.forename: Zarrin
authors.initials: Z
authors.lastname: Es'haghi
authors.forename: Freshte
authors.initials: F
authors.lastname: Babazadeh
mesh_terms: D000079:Acetaldehyde / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D059627:Liquid Phase Microextraction / Q000379:methods*; D010530:Peritoneal Dialysis; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet
chemical_list: D012996:Solutions; D000079:Acetaldehyde