title: In situ supramolecular assembly and modular modification of hyaluronic acid hydrogels for 3D cellular engineering.
abstract: A facile in situ supramolecular assembly and modular modification of biocompatible hydrogels were demonstrated using cucurbit[6]uril-conjugated hyaluronic acid (CB[6]-HA), diaminohexane-conjugated HA (DAH-HA), and tags-CB[6] for cellular engineering applications. The strong and selective host-guest interaction between CB[6] and DAH made possible the supramolecular assembly of CB[6]/DAH-HA hydrogels in the presence of cells. Then, the 3D environment of CB[6]/DAH-HA hydrogels was modularly modified by the simple treatment with various multifunctional tags-CB[6]. Furthermore, we could confirm in situ formation of CB[6]/DAH-HA hydrogels under the skin of nude mice by sequential subcutaneous injections of CB[6]-HA and DAH-HA solutions. The fluorescence of modularly modified fluorescein isothiocyanate (FITC)-CB[6] in the hydrogels was maintained for up to 11 days, reflecting the feasibility to deliver the proper cues for cellular proliferation and differentiation in the body. Taken together, CB[6]/DAH-HA hydrogels might be successfully exploited as a 3D artificial extracellular matrix for various tissue engineering applications.
authors.affiliation: Center for Smart Supramolecules, Department of Chemistry, Division of Advanced Materials Science, Pohang University of Science and Technology (POSTECH), San 31, Hyoja-dong, Nam-gu, Pohang, Kyungbuk 790-784, Korea.
authors.forename: Kyeng Min
authors.initials: KM
authors.lastname: Park
authors.forename: Jeong-A
authors.initials: JA
authors.lastname: Yang
authors.forename: Hyuntae
authors.initials: H
authors.lastname: Jung
authors.forename: Junseok
authors.initials: J
authors.lastname: Yeom
authors.forename: Ji Sun
authors.initials: JS
authors.lastname: Park
authors.forename: Keun-Hong
authors.initials: KH
authors.lastname: Park
authors.forename: Allan S
authors.initials: AS
authors.lastname: Hoffman
authors.forename: Sei Kwang
authors.initials: SK
authors.lastname: Hahn
authors.forename: Kimoon
authors.initials: K
authors.lastname: Kim
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology / Q000633:toxicity; D001952:Bridged-Ring Compounds / Q000737:chemistry; D060846:Cell Engineering / Q000379:methods*; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D006820:Hyaluronic Acid / Q000737:chemistry* / Q000494:pharmacology / Q000633:toxicity; D020100:Hydrogels / Q000737:chemistry*; D007093:Imidazoles / Q000737:chemistry; D051379:Mice; D041681:NIH 3T3 Cells; D009842:Oligopeptides / Q000737:chemistry; D011073:Polyamines / Q000737:chemistry; D047028:Macrocyclic Compounds; D000099246:Cucurbit(n)urils
chemical_list: D001672:Biocompatible Materials; D001952:Bridged-Ring Compounds; D006820:Hyaluronic Acid; D020100:Hydrogels; D007093:Imidazoles; D009842:Oligopeptides; D011073:Polyamines; C047981:arginyl-glycyl-aspartic acid; C454805:cucurbit(6)uril; D047028:Macrocyclic Compounds; D000099246:Cucurbit(n)urils