title: Analysis of cycloserine and related compounds using aqueous normal phase chromatography/mass spectrometry.
abstract: A new approach is evaluated for the analysis of cycloserine, a strongly hydrophilic drug. The method utilized is aqueous normal phase chromatography with a silica hydride-based stationary phase and mass spectrometry for detection. The samples are analyzed to determine the number of components and they are identified when possible. In addition, the composition change is monitored with respect to time and sample solvent. Analyses using both gradient and isocratic conditions are presented. The repeatability of inter- and intraday analyses is also determined.
authors.affiliation: Department of Chemistry, San Jose State University, San Jose, CA 95192, USA. joseph.pesek@sjsu.edu
authors.forename: Joseph J
authors.initials: JJ
authors.lastname: Pesek
authors.forename: Maria T
authors.initials: MT
authors.lastname: Matyska
authors.forename: Andy
authors.initials: A
authors.lastname: Dang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003523:Cycloserine / Q000031:analogs & derivatives* / Q000032:analysis*; D004340:Drug Contamination; D013058:Mass Spectrometry / Q000379:methods; D015203:Reproducibility of Results; D017640:Silicates / Q000737:chemistry; D012997:Solvents / Q000737:chemistry
chemical_list: D017640:Silicates; D012997:Solvents; C487012:silica hydride; D003523:Cycloserine