title: Promising plasmid DNA vector based on APTES-modified silica nanoparticles.
abstract: Nanoparticles have an enormous potential for development in biomedical applications, such as gene or drug delivery. We developed and characterized aminopropyltriethoxysilane-functionalized silicon dioxide nanoparticles (APTES-SiNPs) for gene therapy. Lipofectamine(®) 2000, a commonly used agent, served as a contrast. We showed that APTES-SiNPs had a gene transfection efficiency almost equal to that of Lipofectamine 2000, but with lower cytotoxicity. Thus, these novel APTES-SiNPs can achieve highly efficient transfection of plasmid DNA, and to some extent reduce cytotoxicity, which might overcome the critical drawbacks in vivo of conventional carriers, such as viral vectors, organic polymers, and liposomes, and seem to be a promising nonviral gene therapy vector.
authors.affiliation: Department of Vascular Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
authors.forename: Tuck-yun
authors.initials: TY
authors.lastname: Cheang
authors.forename: Bing
authors.initials: B
authors.lastname: Tang
authors.forename: An-wu
authors.initials: AW
authors.lastname: Xu
authors.forename: Guang-qi
authors.initials: GQ
authors.lastname: Chang
authors.forename: Zuo-jun
authors.initials: ZJ
authors.lastname: Hu
authors.forename: Wei-ling
authors.initials: WL
authors.lastname: He
authors.forename: Zhou-hao
authors.initials: ZH
authors.lastname: Xing
authors.forename: Jian-bo
authors.initials: JB
authors.lastname: Xu
authors.forename: Mian
authors.initials: M
authors.lastname: Wang
authors.forename: Shen-ming
authors.initials: SM
authors.lastname: Wang
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000737:chemistry* / Q000493:pharmacokinetics; D004337:Drug Carriers / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D005822:Genetic Vectors / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008055:Lipids / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D009131:Muscle, Smooth, Vascular; D032389:Myocytes, Smooth Muscle; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011437:Propylamines; D012821:Silanes / Q000737:chemistry* / Q000494:pharmacology; D012822:Silicon Dioxide / Q000737:chemistry*; D014162:Transfection / Q000379:methods*
chemical_list: D004337:Drug Carriers; D008055:Lipids; C086724:Lipofectamine; D011437:Propylamines; D012821:Silanes; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D012822:Silicon Dioxide; D004247:DNA; C477625:amino-propyl-triethoxysilane