title: Correlations between clinicopathological parameters and molecular signatures of primary tumors for patients with stage T3n0 colorectal adenocarcinomas: a single center retrospective study on 100 cases.
abstract: OBJECTIVE
  To examine the clinical and protein expression characteristics of tumor tissues for prediction of prognosis in colorectal cancer (CRC).
  
  
  METHODS
  We retrospectively analyzed the clinicopathological data of patients with stage T3N0 CRC, operated between 1997-2003 and the surgical materials for the relation between disease prognosis and p53, p21, p16, β-catenin, E-cadherin, EGFR, hMLH1, hMSH2 and TS protein expressions.
  
  
  RESULTS
  A significantly shorter 3-year disease free survival was observed in patients under the age of 50. The worst 5-year overall survival (OS) observed for patients over 70. Tumor localization and number of processed lymph nodes significantly affected prognosis. The EGFR, hMSH2 and TS expressions and the 5-fluorouracyl treatment were not found to be of prognostic value; p53 and p21 positivity had significantly worse survival. When β-catenin membrane expression disappeared on tumor cells, the 5-year OS rate decreased and time to metastasis shortened significantly. Membrane β-catenin expression, processed lymph nodes number and age were detected as independent prognostic markers.
  
  
  CONCLUSIONS
  These results suggest that the evaluation of a clinicopathological profile, based on age, tumor localization, number of examined lymph nodes, p53, p21 and E-cadherin β-catenin expression appears to be useful in identifying high risk patients.
authors.affiliation: Department of Oncology, University of Debrecen, Debrecen, Hungary.
authors.forename: Csilla
authors.initials: C
authors.lastname: András
authors.forename: László
authors.initials: L
authors.lastname: Tóth
authors.forename: Csaba
authors.initials: C
authors.lastname: Molnár
authors.forename: Miklós
authors.initials: M
authors.lastname: Tanyi
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Csiki
authors.forename: Balázs
authors.initials: B
authors.lastname: Dezso
authors.forename: János
authors.initials: J
authors.lastname: Pósán
authors.forename: Amir-Houshang
authors.initials: AH
authors.lastname: Shemirani
authors.forename: Emese
authors.initials: E
authors.lastname: Csiki
authors.forename: János
authors.initials: J
authors.lastname: Szántó
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry / Q000401:mortality / Q000473:pathology*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015820:Cadherins / Q000032:analysis; D015179:Colorectal Neoplasms / Q000737:chemistry / Q000401:mortality / Q000473:pathology*; D066246:ErbB Receptors / Q000032:analysis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D012189:Retrospective Studies; D013940:Thymidylate Synthase / Q000032:analysis; D016159:Tumor Suppressor Protein p53 / Q000032:analysis; D051176:beta Catenin / Q000032:analysis
chemical_list: C495270:CTNNB1 protein, human; D015820:Cadherins; D016159:Tumor Suppressor Protein p53; D051176:beta Catenin; D013940:Thymidylate Synthase; D066246:ErbB Receptors