title: Intracellular conformations of human telomeric quadruplexes studied by electron paramagnetic resonance spectroscopy.
abstract: In-cell DEER (in-cell double electron electron resonance) is applied to investigate quadruplex formation of the human telomeric repeat d[AGGG(TTAGGG)(3)]. The initially unfolded DNA sequence forms a mixture of different quadruplex topologies upon injection into living cells. In addition, time-dependent distance measurements are carried out to monitor quadruplex folding.
authors.affiliation: Department of Chemistry, Konstanz Research School Chemical Biology (KoRS-CB) and Zukunftskolleg, University of Konstanz, Konstanz, Germany.
authors.forename: Mykhailo
authors.initials: M
authors.lastname: Azarkh
authors.forename: Vijay
authors.initials: V
authors.lastname: Singh
authors.forename: Oliver
authors.initials: O
authors.lastname: Okle
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Dietrich
authors.forename: Jörg S
authors.initials: JS
authors.lastname: Hartig
authors.forename: Malte
authors.initials: M
authors.lastname: Drescher
mesh_terms: D001483:Base Sequence; D004578:Electron Spin Resonance Spectroscopy; D006801:Humans; D009690:Nucleic Acid Conformation; D016615:Telomere / Q000737:chemistry* / Q000235:genetics