title: Significance of the conformation of building blocks in curing of barnacle underwater adhesive.
abstract: Barnacles are a unique sessile crustacean that attach irreversibly and firmly to foreign underwater surfaces. Its biological underwater adhesive is a peculiar extracellular multi-protein complex. Here we characterize one of the two major proteins, a 52 kDa protein found in the barnacle cement complex. Cloning of the cDNA revealed that the protein has no homolog in the nonredundant database. The primary structure consists of four long sequence repeats. The process of dissolving the protein at the adhesive joint of the animal by various treatments was monitored in order to obtain insight into the molecular mechanism involved in curing of the adhesive bulk. Treatments with protein denaturant, reducing agents and/or chemical-specific proteolysis in combination with 2D diagonal PAGE indicated no involvement of the protein in intermolecular cross-linkage/polymerization, including formation of intermolecular disulfide bonds. As solubilization of the proteins required high concentrations of denaturing agents, it appears that both the conformation of the protein as building blocks and non-covalent molecular interactions between the building blocks, possibly hydrophobic interactions and hydrogen bonds, are crucial for curing of the cement. It was also suggested that the protein contributes to surface coupling by an anchoring effect to micro- to nanoscopic roughness of surfaces.
authors.affiliation: Marine Biotechnology Institute, Iwate, Japan. kamino-kei@nite.go.jp
authors.forename: Kei
authors.initials: K
authors.lastname: Kamino
authors.forename: Masahiro
authors.initials: M
authors.lastname: Nakano
authors.forename: Satoru
authors.initials: S
authors.lastname: Kanai
mesh_terms: D000269:Adhesives; D000595:Amino Acid Sequence; D000818:Animals; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D057927:Hydrophobic and Hydrophilic Interactions; D008969:Molecular Sequence Data; D046912:Multiprotein Complexes / Q000737:chemistry* / Q000378:metabolism; D001468:Thoracica / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000269:Adhesives; D018076:DNA, Complementary; D046912:Multiprotein Complexes