title: The interaction of serum proteins with carbon nanotubes depend on the physicochemical properties of nanotubes.
abstract: With more and more potential applications of carbon nanotubes (CNTs) in different fields, the risk of exposure to CNTs is increasing. The interaction between CNTs and protein in biological media can affect the way cells interact with, recognize and process the nanoparticles, and this has important implications for safety considerations. In this study, the interaction of single-walled and multiwall CNTs with various serum proteins was investigated. The adsorption kinetics of protein to CNTs was investigated and a semi-qualitative analysis was provided by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Matrix assisted laser desorption ionization/time of flight mass spectrometry (MALDI-TOF MS) was used to identify the protein species binding to CNTs and atomic force microscopy (AFM) was used to vividly demonstrate the adsorption model of protein on CNTs. All the experimental results showed that the adsorption capacity of CNTs for protein was highly dependent on the type, arrangement model, size and surface modification of CNTs. Significant quantity of proteins in serum could be quickly adsorbed by CNTs, mainly including albumin, prealbumin, transferrin, and immunoglobulin. Noncovalent functionalization of CNTs by polyethylene glycol (PEG) could decrease the protein adsorption on CNTs. These results provide crucial insights into human serum proteins binding to different kinds of CNTs, which is important for understanding the safe application of carbon nanotubes.
authors.affiliation: College of Chemistry and Chemical Engineering, University of South China, Hengyang, Hunan 421001, P. R. China.
authors.forename: Jiangfeng
authors.initials: J
authors.lastname: Du
authors.forename: Cuicui
authors.initials: C
authors.lastname: Ge
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Ru
authors.initials: R
authors.lastname: Bai
authors.forename: Denghua
authors.initials: D
authors.lastname: Li
authors.forename: Yanlian
authors.initials: Y
authors.lastname: Yang
authors.forename: Lifu
authors.initials: L
authors.lastname: Liao
authors.forename: Chunying
authors.initials: C
authors.lastname: Chen
mesh_terms: D000327:Adsorption; D000818:Animals; D001798:Blood Proteins / Q000737:chemistry* / Q000378:metabolism; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D011092:Polyethylene Glycols; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001798:Blood Proteins; D037742:Nanotubes, Carbon; D011092:Polyethylene Glycols