title: Silencing of CCDC6 reduces the expression of 14-3-3σ in colorectal carcinoma cells.
abstract: Coiled-coil domain containing 6 (CCDC6) is frequently rearranged in papillary thyroid carcinomas participating in the formation of RET/PTC1 oncogene. Other rearrangements involving CCDC6 have also been identified demonstrating its high susceptibility to chromosomal recombination. Malignancies bearing CCDC6 fusion genes are developed in a background where CCDC6 is either lost or deregulated. Our aim was to identify interacting proteins which are affected by the silencing of CCDC6 expression and could possibly link CCDC6 deregulation to cancer causality. Therefore, a proteomic approach was adopted using a human cancer cell-line (HCT116) where CCDC6 expression was silenced by lentiviral shRNA constructs. 14-3-3σ down-regulation in the absence of CCDC6 was revealed and verified by western blot analysis and confocal microscopy. Only the levels and not the topology of CCDC6 were altered. The down-regulation of 14-3-3σ in the absence of CCDC6 demonstrated their direct association and supports the notion that CCDC6 contributes to cancer development, possibly through malignant pathways involving 14-3-3σ.
authors.affiliation: Department of Genetics and Gene Therapy Unit, Biomedical Research Foundation of the Academy of Athens, Greece.
authors.forename: Angeliki
authors.initials: A
authors.lastname: Thanasopoulou
authors.forename: Alexandra G
authors.initials: AG
authors.lastname: Xanthopoulou
authors.forename: Athanasios K
authors.initials: AK
authors.lastname: Anagnostopoulos
authors.forename: Eumorphia G
authors.initials: EG
authors.lastname: Konstantakou
authors.forename: Lukas H
authors.initials: LH
authors.lastname: Margaritis
authors.forename: Isidora S
authors.initials: IS
authors.lastname: Papassideri
authors.forename: Dimitrios J
authors.initials: DJ
authors.lastname: Stravopodis
authors.forename: George T
authors.initials: GT
authors.lastname: Tsangaris
authors.forename: Ema
authors.initials: E
authors.lastname: Anastasiadou
mesh_terms: D048948:14-3-3 Proteins / Q000378:metabolism*; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000378:metabolism*; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology; D003598:Cytoskeletal Proteins / Q000235:genetics*; D017931:DNA Primers; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional; D005092:Exonucleases / Q000378:metabolism*; D005095:Exoribonucleases; D055785:Gene Knockdown Techniques; D020868:Gene Silencing; D006801:Humans; D040901:Proteomics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D048948:14-3-3 Proteins; D014408:Biomarkers, Tumor; C088832:CCDC6 protein, human; D003598:Cytoskeletal Proteins; D017931:DNA Primers; D005092:Exonucleases; D005095:Exoribonucleases; C413442:SFN protein, human