title: Detection of antiphospholipid antibodies by automated chemiluminescence assay.
abstract: The laboratory diagnosis of antiphospholipid antibody syndrome (APS) requires the demonstration of antiphospholipid antibodies (aPL) by lupus anticoagulant (LAC) measured through coagulation assays, anticardiolipin IgG or IgM antibodies (aCL) and/or anti-β2-glycoprotein I IgG or IgM antibodies (anti-β2-GPI), usually detected by ELISA. In this study we tested aCL by a new automated system using the chemiluminescence principle. Our results showed that, while almost all the sera from APS patients, positive for IgG aCL and anti-β2-GPI by ELISA, were also positive for IgG aCl by chemiluminescence, only 30.13% of patients without clinical manifestations of APS, but positive for aCL and persistently negative for anti-β2-GPI (by ELISA) and LA, confirmed the positive test by chemiluminescence. This difference was highly significant (p<0.0001). Interestingly, this test also prompted to identify 20% of patients positive for LA, but persistently negative for both aCL and anti-β2-GPI IgG (ELISA). Thus, the new technology of automated chemiluminescence assay for measuring aPL may represent an useful tool to identify "true" APS patients.
authors.affiliation: Dipartimento di Medicina Sperimentale, “Sapienza” University, Rome, Italy.
authors.forename: Antonella
authors.initials: A
authors.lastname: Capozzi
authors.forename: Emanuela
authors.initials: E
authors.lastname: Lococo
authors.forename: Maria
authors.initials: M
authors.lastname: Grasso
authors.forename: Agostina
authors.initials: A
authors.lastname: Longo
authors.forename: Tina
authors.initials: T
authors.lastname: Garofalo
authors.forename: Roberta
authors.initials: R
authors.lastname: Misasi
authors.forename: Maurizio
authors.initials: M
authors.lastname: Sorice
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D017152:Antibodies, Antiphospholipid / Q000032:analysis*; D016736:Antiphospholipid Syndrome / Q000175:diagnosis; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods*; D008297:Male; D008875:Middle Aged
chemical_list: D017152:Antibodies, Antiphospholipid