title: Standardized one-step real-time reverse transcription-PCR assay for universal detection and quantification of hepatitis delta virus from clinical samples in the presence of a heterologous internal-control RNA.
abstract: As for other chronic viral diseases, quantification of hepatitis delta virus (HDV) loads may be useful for patient management. We describe a one-step quantitative reverse transcription-PCR assay that is reliable and automatable and meets the regulatory authorities' standards for accurate quantification of the major HDV genotypes. It includes an internal control and uses in vitro-transcribed RNAs as standards. Its linearity range is 500 to 1.7 × 10(11) copies/ml, its sensitivity is around 150 copies/ml, its repeatability is around 15%, and its reproducibility is below 0.25 log(10) copies/ml.
authors.affiliation: Hospices Civils de Lyon, Hôpital de la Croix Rousse, Laboratoire de Virologie, Lyon, France. caroline.scholtes@inserm.fr
authors.forename: Caroline
authors.initials: C
authors.lastname: Scholtes
authors.forename: Vinca
authors.initials: V
authors.lastname: Icard
authors.forename: Majid
authors.initials: M
authors.lastname: Amiri
authors.forename: Philippe
authors.initials: P
authors.lastname: Chevallier-Queyron
authors.forename: Mary-Anne
authors.initials: MA
authors.lastname: Trabaud
authors.forename: Christophe
authors.initials: C
authors.lastname: Ramière
authors.forename: Fabien
authors.initials: F
authors.lastname: Zoulim
authors.forename: Patrice
authors.initials: P
authors.lastname: André
authors.forename: Paul
authors.initials: P
authors.lastname: Dény
mesh_terms: D003699:Hepatitis D / Q000821:virology*; D003698:Hepatitis Delta Virus / Q000302:isolation & purification*; D006801:Humans; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods* / Q000592:standards*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019562:Viral Load / Q000379:methods*