title: Dynamic and high-resolution live cell imaging by direct electron beam excitation.
abstract: We propose a direct electron-beam excitation assisted optical microscope with a resolution of a few tens of nanometers and it can be applied for observation of dynamic movements of nanoparticles in liquid. The technique is also useful for live cell imaging under physiological conditions as well as observation of colloidal solution, microcrystal growth in solutions, etc. In the microscope, fluorescent materials are directly excited with a focused electron beam. The direct excitation with an electron beam yields high spatial resolution since the electron beam can be focused to a few tens of nanometers in the specimens. In order to demonstrate the potential of our proposed microscope, we observed the movements of fluorescent nanoparticles, which can be used for labelling specimens, in a water-based solution. We also demonstrated an observation result of living CHO cells.
authors.affiliation: Graduate School of Science and Technology, Shizuoka University, Hamamatsu, 4328561, Japan.
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Nawa
authors.forename: Wataru
authors.initials: W
authors.lastname: Inami
authors.forename: Akito
authors.initials: A
authors.lastname: Chiba
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ono
authors.forename: Atsuo
authors.initials: A
authors.lastname: Miyakawa
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Kawata
authors.forename: Sheng
authors.initials: S
authors.lastname: Lin
authors.forename: Susumu
authors.initials: S
authors.lastname: Terakawa
mesh_terms: D002465:Cell Movement / Q000502:physiology*; D058948:Cell Tracking / Q000379:methods*; D004583:Electrons; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D008856:Microscopy, Fluorescence / Q000295:instrumentation*