title: Molecular evolution of a malaria resistance gene (DARC) in primates.
abstract: Genes involved in host-pathogen interactions are often strongly affected by positive natural selection. The Duffy antigen, coded by the Duffy antigen receptor for chemokines (DARC) gene, serves as a receptor for Plasmodium vivax in humans and for Plasmodium knowlesi in some nonhuman primates. In the majority of sub-Saharan Africans, a nucleic acid variant in GATA-1 of the gene promoter is responsible for the nonexpression of the Duffy antigen on red blood cells and consequently resistance to invasion by P. vivax. The Duffy antigen also acts as a receptor for chemokines and is expressed in red blood cells and many other tissues of the body. Because of this dual role, we sequenced a ~3,000-bp region encompassing the entire DARC gene as well as part of its 5' and 3' flanking regions in a phylogenetic sample of primates and used statistical methods to evaluate the nature of selection pressures acting on the gene during its evolution. We analyzed both coding and regulatory regions of the DARC gene. The regulatory analysis showed accelerated rates of substitution at several sites near known motifs. Our tests of positive selection in the coding region using maximum likelihood by branch sites and maximum likelihood by codon sites did not yield statistically significant evidence for the action of positive selection. However, the maximum likelihood test in which the gene was subdivided into different structural regions showed that the known binding region for P. vivax/P. knowlesi is under very different selective pressures than the remainder of the gene. In fact, most of the gene appears to be under strong purifying selection, but this is not evident in the binding region. We suggest that the binding region is under the influence of two opposing selective pressures, positive selection possibly exerted by the parasite and purifying selection exerted by chemokines.
authors.affiliation: Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065, USA. stratus@lgmb.fmrp.usp.br
authors.forename: Thiago Yukio Kikuchi
authors.initials: TY
authors.lastname: Oliveira
authors.forename: Eugene E
authors.initials: EE
authors.lastname: Harris
authors.forename: Diogo
authors.initials: D
authors.lastname: Meyer
authors.forename: Chong K
authors.initials: CK
authors.lastname: Jue
authors.forename: Wilson Araújo
authors.initials: WA
authors.lastname: Silva
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D060467:Disease Resistance / Q000235:genetics*; D004375:Duffy Blood-Group System / Q000235:genetics* / Q000378:metabolism; D019143:Evolution, Molecular; D050982:GATA1 Transcription Factor / Q000378:metabolism; D006801:Humans; D008288:Malaria / Q000235:genetics / Q000469:parasitology; D008969:Molecular Sequence Data; D010802:Phylogeny; D010966:Plasmodium vivax / Q000472:pathogenicity*; D011323:Primates / Q000235:genetics*; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid; D012641:Selection, Genetic; D016415:Sequence Alignment
chemical_list: C473745:ACKR1 protein, human; D004375:Duffy Blood-Group System; D050982:GATA1 Transcription Factor; D011956:Receptors, Cell Surface