title: Inhibition of acetylcholinesterase activity and Fe2+-induced lipid peroxidation in rat brain in vitro by some citrus fruit juices.
abstract: This study sought to characterize the effects of some citrus fruit juices (shaddock [Citrus maxima], grapefruit [Citrus paradisii], lemon [Citrus limoni], orange [Citrus sinensis], and tangerine [Citrus reticulata]) on acetylcholinesterase activity in vitro. The total phenolic content, radical scavenging abilities, and inhibition of Fe(2+)-induced malondialdehyde (MDA) production in rats brain homogenate in vitro were also assessed. Orange had significantly (P<.05) higher phenolic content than the other juices. The juices scavenged 1,1-diphenyl-2-picrylhydrazyl and hydroxyl radicals in a dose-dependent manner with orange having the highest scavenging ability. Furthermore, the juices inhibited Fe(2+)-induced MDA production in rat brain homogenate in a dose-dependent manner with shaddock having the highest inhibitory ability. Acetylcholinesterase activity was also inhibited in vitro by the juices in a dose-dependent manner. The inhibition of acetylcholinesterase activity and antioxidant properties of the citrus juices could make them a good dietary means for the management of Alzheimer's disease.
authors.affiliation: Department of Biochemistry, Federal University of Technology, Akure, Ondo-State, Nigeria.
authors.forename: Ayokunle O
authors.initials: AO
authors.lastname: Ademosun
authors.forename: Ganiyu
authors.initials: G
authors.lastname: Oboh
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000544:Alzheimer Disease / Q000188:drug therapy / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001628:Beverages; D001713:Biphenyl Compounds / Q000378:metabolism; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D002957:Citrus; D004305:Dose-Response Relationship, Drug; D005638:Fruit / Q000737:chemistry; D007501:Iron / Q000009:adverse effects; D015227:Lipid Peroxidation / Q000187:drug effects*; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010636:Phenols / Q000032:analysis / Q000494:pharmacology*; D010851:Picrates / Q000378:metabolism; D028321:Plant Preparations / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D002800:Cholinesterase Inhibitors; D010636:Phenols; D010851:Picrates; D028321:Plant Preparations; D008315:Malondialdehyde; C004931:1,1-diphenyl-2-picrylhydrazyl; D007501:Iron; D000110:Acetylcholinesterase