title: Differential responses of white adipose tissue and brown adipose tissue to caloric restriction in rats.
abstract: Caloric restriction (CR) slows the aging process and extends longevity, but the exact underlying mechanisms remain debatable. It has recently been suggested that the beneficial action of CR may be mediated in part by adipose tissue remodeling. Mammals have two types of adipose tissue: white adipose tissue (WAT) and brown adipose tissue (BAT). In this study, proteome analysis using two-dimensional gel electrophoresis combined with MALDI-TOF MS, and subsequent analyses were performed on both WAT and BAT from 9-month-old male rats fed ad libitum or subjected to CR for 6 months. Our findings suggest that CR activates mitochondrial energy metabolism and fatty acid biosynthesis in WAT. It is likely that in CR animals WAT functions as an energy transducer from glucose to energy-dense lipid. In contrast, in BAT CR either had no effect on, or down-regulated, the mitochondrial electron transport chain, but enhanced fatty acid biosynthesis. This suggests that in CR animals BAT may change its function from an energy consuming system to an energy reservoir system. Based on our findings, we conclude that WAT and BAT cooperate to use energy effectively via a differential response of mitochondrial function to CR.
authors.affiliation: Molecular Pathology & Metabolic Disease, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Tokyo, Japan.
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Okita
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Hayashida
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Kojima
authors.forename: Mayumi
authors.initials: M
authors.lastname: Fukushima
authors.forename: Keiko
authors.initials: K
authors.lastname: Yuguchi
authors.forename: Kentaro
authors.initials: K
authors.lastname: Mikami
authors.forename: Akiko
authors.initials: A
authors.lastname: Yamauchi
authors.forename: Kyoko
authors.initials: K
authors.lastname: Watanabe
authors.forename: Mituru
authors.initials: M
authors.lastname: Noguchi
authors.forename: Megumi
authors.initials: M
authors.lastname: Nakamura
authors.forename: Toshifusa
authors.initials: T
authors.lastname: Toda
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Higami
mesh_terms: D002001:Adipose Tissue, Brown / Q000378:metabolism*; D052436:Adipose Tissue, White / Q000378:metabolism*; D000818:Animals; D031204:Caloric Restriction; D015536:Down-Regulation; D004734:Energy Metabolism / Q000502:physiology; D005227:Fatty Acids / Q000096:biosynthesis; D008297:Male; D008928:Mitochondria / Q000378:metabolism; D020543:Proteome; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005227:Fatty Acids; D020543:Proteome