title: Functionalization of single-walled carbon nanotubes enables efficient intracellular delivery of siRNA targeting MDM2 to inhibit breast cancer cells growth.
abstract: The delivery of DNA or RNA to cells represents the limiting step in the development of cancer gene therapy and RNA interference protocols. Single walled carbon nanotubes (SWNTs) are of interest as carriers of biologically active molecules because of their ability to cross cell membranes. In this study, we developed a novel strategy for chemical functionalization of SWNTs (f-SWCNTs) with DSPE-PEG-Amine to bind small interfering RNA (siRNA) by disulfide bonds applied to siRNA-mediated gene silencing in breast cancer cells. Results indicated the efficiency of f-SWNTs carrying siRNA reached 83.55%, and the new f-SWNTs-siRNA-MDM2 complexes were successfully introduced into the breast carcinoma B-Cap-37 cells at a concentration of 100 nM in mediums, and caused proliferation inhibition of B-Cap-37 cells significantly. The proliferation inhibition ratio of B-Cap-37 cells was detected as 44.53% for 72 h, and the apoptosis ratio was measured as 30.45%. It was obvious that MDM2 can serve as a novel therapeutic target by an effective carrier system of DSPE-PEG-Amine-functionalized SWNTs, which would be very advanced and significant to therapy of breast cancer further.
authors.affiliation: School of Biotechnology, State Key Laboratory of Bioreactor engineering, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Hailong
authors.initials: H
authors.lastname: Chen
authors.forename: Xingyuan
authors.initials: X
authors.lastname: Ma
authors.forename: Zhi
authors.initials: Z
authors.lastname: Li
authors.forename: Qiaoyun
authors.initials: Q
authors.lastname: Shi
authors.forename: Wenyun
authors.initials: W
authors.lastname: Zheng
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
mesh_terms: D000588:Amines / Q000737:chemistry; D017209:Apoptosis; D001943:Breast Neoplasms / Q000235:genetics / Q000628:therapy*; D049109:Cell Proliferation; D005260:Female; D020868:Gene Silencing; D015316:Genetic Therapy / Q000379:methods; D006801:Humans; D037742:Nanotubes, Carbon; D010714:Phosphatidylethanolamines / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D051736:Proto-Oncogene Proteins c-mdm2 / Q000235:genetics*; D034741:RNA, Small Interfering / Q000008:administration & dosage*; D013997:Time Factors
chemical_list: D000588:Amines; D037742:Nanotubes, Carbon; D010714:Phosphatidylethanolamines; D034741:RNA, Small Interfering; C107570:polyethylene glycol-distearoylphosphatidylethanolamine; D011092:Polyethylene Glycols; C497206:MDM2 protein, human; D051736:Proto-Oncogene Proteins c-mdm2