title: Streptavidin suppresses T cell activation and inhibits IL-2 production and CD25 expression.
abstract: Streptavidin is widely used as a detection tool in biology research because of its high affinity and specificity binding to biotin. Biotin-streptavidin system has also been explored for detection of infection and tumor in clinical medicine. Here, we show immunosuppressive property of streptavidin on T cell activation and proliferation. Upon CD3 and CD28 stimulation, CD4(+) T cells produce interleukin 2 (IL-2) and express IL-2 receptor α chain (CD25). Addition of streptavidin in T cell culture suppressed IL-2 synthesis and CD25 expression with no cytotoxicity. The immunosuppressive effect of streptavidin was reversed by excessive biotin. Conjugated to a single chain anti-CD7 variable fragment (scFvCD7), streptavidin was directly delivered to T cells and showed substantially more profound suppressive effect on T cell activation. These results suggest that streptavidin could potentially be used as a novel immunomodulator.
authors.affiliation: Division of Arthritis and Rheumatic Disease, Oregon Health & Science University, OR, USA.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Yomogida
authors.forename: Yuan
authors.initials: Y
authors.lastname: Chou
authors.forename: Jonathan
authors.initials: J
authors.lastname: Pang
authors.forename: Bobby
authors.initials: B
authors.lastname: Baravati
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Maniaci
authors.forename: Shili
authors.initials: S
authors.lastname: Wu
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhu
authors.forename: Cong-Qiu
authors.initials: CQ
authors.lastname: Chu
mesh_terms: D049109:Cell Proliferation; D005434:Flow Cytometry; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis*; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism*; D008213:Lymphocyte Activation / Q000187:drug effects*; D060888:Real-Time Polymerase Chain Reaction; D019809:Streptavidin / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism
chemical_list: D007376:Interleukin-2; D053645:Interleukin-2 Receptor alpha Subunit; D019809:Streptavidin