title: A rapid method to regenerate piezoelectric microcantilever sensors (PEMS).
abstract: Piezoelectric microcantilever sensors (PEMS) can be sensitive tools for the detection of proteins and cells in biological fluids. However, currently available PEMS can only be used a single time or must be completely stripped and refunctionalized prior to subsequent uses. Here we report the successful use of an alternative regeneration protocol employing high salt concentrations to remove the target, leaving the functional probe immobilized on the microcantilever surface. Our model system employed the extracellular domain (ECD) of recombinant human Epidermal Growth Factor Receptor (EGFR) as the probe and anti-human EGFR polyclonal antibodies as the target. We report that high concentrations of MgCl2 dissociated polyclonal antibodies specifically bound to EGFR ECD immobilized on the sensor surface without affecting its bioactivity. This simple regeneration protocol both minimized the time required to re-conjugate the probe and preserved the density of probe immobilized on PEMS surface, yielding identical biosensor sensitivity over a series of assays.
authors.affiliation: Developmental Therapeutics Program, Fox Chase Cancer Center, 333 Cottman Ave., Philadelphia, PA 19111, USA. lina.loo@fccc.edu
authors.forename: LiNa
authors.initials: L
authors.lastname: Loo
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.forename: Wan Y
authors.initials: WY
authors.lastname: Shih
authors.forename: Wei-Heng
authors.initials: WH
authors.lastname: Shih
authors.forename: Hossein
authors.initials: H
authors.lastname: Borghaei
authors.forename: Kambiz
authors.initials: K
authors.lastname: Pourrezaei
authors.forename: Gregory P
authors.initials: GP
authors.lastname: Adams
mesh_terms: D000906:Antibodies / Q000737:chemistry; D000941:Antigens / Q000737:chemistry; D015415:Biomarkers / Q000032:analysis; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D006801:Humans
chemical_list: D000906:Antibodies; D000941:Antigens; D015415:Biomarkers