title: Separation and identification of highly fluorescent compounds derived from trans-resveratrol in the leaves of Vitis vinifera infected by Plasmopara viticola.
abstract: A method for identification of highly fluorescent compounds in vine leaves infected by Plasmopara viticola was developed using reversed phase liquid chromatography with simultaneous diode array and fluorometric detection. Fluorescent compounds were extracted from leaves with a methanol-water mixture (70:30). Separation by HPLC was performed using a C(18) column and gradient elution with water-acetonitrile mixtures (20-80% of acetonitrile). The main unknown fluorescent compound was identified by line spectral comparison with a standard obtained by UV photoisomerization of trans-resveratrol glucoside, and its structure was confirmed by liquid chromatography-mass spectrometry. Identification and structural elucidation of the fluorescent compound in the leaves of Vitis vinifera allows early detection of Plasmopara viticola invasion.
authors.affiliation: Laboratory of Metabolomics and Isotopic Analyses, Global Change Research Center, Academy of Sciences of the Czech Republic, Branišovská 31, České Budějovice 370 05, Czech Republic. triska.j@czechglobe.cz
authors.forename: Jan
authors.initials: J
authors.lastname: Tříska
authors.forename: Naděžda
authors.initials: N
authors.lastname: Vrchotová
authors.forename: Julie
authors.initials: J
authors.lastname: Olejníčková
authors.forename: Rudolf
authors.initials: R
authors.lastname: Jílek
authors.forename: Radek
authors.initials: R
authors.lastname: Sotolář
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid / Q000592:standards; D005456:Fluorescent Dyes / Q000737:chemistry / Q000302:isolation & purification*; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification* / Q000528:radiation effects; D007536:Isomerism; D013058:Mass Spectrometry / Q000592:standards; D044742:Peronospora; D010616:Phenanthrenes / Q000737:chemistry / Q000302:isolation & purification*; D010935:Plant Diseases / Q000382:microbiology*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification*; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism / Q000382:microbiology*; D012015:Reference Standards; D000077185:Resveratrol; D013267:Stilbenes / Q000737:chemistry* / Q000528:radiation effects; D014466:Ultraviolet Rays; D027843:Vitis / Q000737:chemistry / Q000378:metabolism / Q000382:microbiology*
chemical_list: C572848:2,4,6-trihydroxyphenanthrene-2-O-glucoside; D005456:Fluorescent Dyes; D005960:Glucosides; D010616:Phenanthrenes; D010936:Plant Extracts; D013267:Stilbenes; D000077185:Resveratrol; C058229:polydatin