title: Sweet and bitter tastants specific detection by the taste cell-based sensor.
abstract: Sweet and bitter tastants specific detection by cell-based sensor is investigated in this paper. Human enteroendocrine NCI-H716 cells, expressing G protein-coupled receptors and sweet receptors (type 1, member 2/type 1, member 3), and human enteroendocrine STC-1 cells, expressing G protein-coupled receptors and bitter receptors (type 2 members) are used as sensing devices. The HEK-293 cells, without taste receptor expression, are used as negative control. The electrochemical impedance spectrum data is recorded and processed by bistable stochastic resonance for signal-to-noise ratio calculation. NCI-H716 cell-based sensor selectively responds to sweeteners and sweet tastant mixtures. STC-1 cell-based sensor selectively responds to bitter tastants and bitter tastant mixtures. The tastants species and concentrations can be decided by signal-to-noise ratio parameters. HEK-293 cell-based sensor lacks the tastants discriminating ability. The taste cell-based sensor is easy to prepare and operate. This work offers a useful way in gustatory mechanism research.
authors.affiliation: Sensory Science Laboratory, College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310035, PR China.
authors.forename: Guo-Hua
authors.initials: GH
authors.lastname: Hui
authors.affiliation: Sensory Science Laboratory, College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310035, PR China.
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Mi
authors.affiliation: Sensory Science Laboratory, College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310035, PR China. Electronic address: spdeng@zjgsu.edu.cn.
authors.forename: Shao-Ping
authors.initials: SP
authors.lastname: Deng
mesh_terms: D015374:Biosensing Techniques / Q000379:methods* / Q000706:statistics & numerical data; D002241:Carbohydrates / Q000494:pharmacology; D002460:Cell Line; D057809:HEK293 Cells; D006801:Humans; D011803:Quinine / Q000494:pharmacology; D043562:Receptors, G-Protein-Coupled / Q000502:physiology; D059629:Signal-To-Noise Ratio; D013269:Stochastic Processes; D013549:Sweetening Agents / Q000494:pharmacology; D013649:Taste / Q000502:physiology; D013650:Taste Buds / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D000098706:Taste Receptors, Type 2
chemical_list: D002241:Carbohydrates; D043562:Receptors, G-Protein-Coupled; D013549:Sweetening Agents; C431926:taste receptors, type 1; D000098706:Taste Receptors, Type 2; D011803:Quinine