title: Separation and identification of volatile compounds from liquid cultures of Trichoderma harzianum by GC-MS using three different capillary columns.
abstract: A simple, fast, repeatable and less laborious sample preparation protocol was developed and applied for the analysis of biocontrol fungus Trichoderma harzianum strain FA1132 by using gas chromatography-mass spectrometry. The match factors for sample spectra with respect to the mass spectra library of fungal volatile compounds were determined and used to study the complex hydrocarbons and other volatile compounds, which were separated by using different capillary columns with nonpolar, medium polar and high polar stationary phases. To date, more than 278 volatile compounds (with spectral match factor at least 90%) such as normal saturated hydrocarbons (C7-C30), cyclohexane, cyclopentane, fatty acids, alcohols, esters, sulfur-containing compounds, simple pyrane and benzene derivatives have been identified. Most of these compounds have not previously been reported. The method described in this paper is a more convenient research tool for the detection of volatile compounds from the cultures of T. harzianum.
authors.affiliation: Biotechnology Research Institute, Universiti Malaysia Sabah, JLN UMS, 88400 Kota Kinabalu, Sabah, Malaysia. shafiqpab@yahoo.com
authors.forename: Shafiquzzaman
authors.initials: S
authors.lastname: Siddiquee
authors.forename: Bo Eng
authors.initials: BE
authors.lastname: Cheong
authors.forename: Khanam
authors.initials: K
authors.lastname: Taslima
authors.forename: Hossain
authors.initials: H
authors.lastname: Kausar
authors.forename: Md Mainul
authors.initials: MM
authors.lastname: Hasan
mesh_terms: D019149:Bioreactors; D017077:Culture Media, Conditioned; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D000432:Methanol; D014242:Trichoderma / Q000378:metabolism*; D055549:Volatile Organic Compounds / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D017077:Culture Media, Conditioned; D055549:Volatile Organic Compounds; D000432:Methanol