title: Predictive value of the vitamin K epoxide reductase complex subunit 1 G-1639A and C1173T single nucleotide polymorphisms in retinal vein occlusion.
abstract: BACKGROUND
  To determine if vitamin K epoxide reductase complex subunit 1 gene polymorphisms have an effect on the risk of having a retinal vein occlusion.
  
  
  METHODS
  Case-control study.
  
  
  METHODS
  The study population consisted of 68 patients who were newly diagnosed with retinal vein occlusion and 66 sex-matched controls.
  
  
  METHODS
  Genomic DNA was extracted from peripheral leukocytes from ethylenediamine tetra-acetic acid-anticoagulated blood. Genotyping of the vitamin K epoxide reductase complex subunit 1 G-1639A (rs 9923231) and C1173T (rs 9934438) single nucleotide polymorphisms was performed using real-time polymerase chain reaction and commercially available kits.
  
  
  METHODS
  A full ophthalmological evaluation was performed in each subject, and all subjects were screened for hypertension, hypercholesterolaemia and diabetes. The genotypes of the vitamin K epoxide reductase complex subunit 1 single nucleotide polymorphisms were determined.
  
  
  RESULTS
  The vitamin K epoxide reductase complex subunit 1 GG and CC genotypes were more frequent (41% vs. 21%; P = 0.021), and the combined GA/AA and CT/CC genotypes were less frequent in patients with retinal vein occlusion than in control subjects. After adjusting for hypertension, age, plasma fibrinogen levels and prevalence of diabetes and hypercholesterolaemia, the GG and CC genotypes were found to be an independent predictor of retinal vein occlusion (B = 2.28; odds ratio = 9.79; P = 0.003; 95% confidence interval: 2.22-43.24).
  
  
  CONCLUSIONS
  It was found that subjects with the vitamin K epoxide reductase complex subunit 1 GG and CC genotypes had a higher risk of retinal vein occlusion.
authors.affiliation: Department of Ophthalmology, Gaziosmanpasa University, Faculty of Medicine, Tokat, Turkey. huseyin.ortak@hotmail.com
authors.forename: Huseyin
authors.initials: H
authors.lastname: Ortak
authors.forename: Erkan
authors.initials: E
authors.lastname: Söğüt
authors.forename: Helin
authors.initials: H
authors.lastname: Demir
authors.forename: Aylin
authors.initials: A
authors.lastname: Ardagil
authors.forename: Ismail
authors.initials: I
authors.lastname: Benli
authors.forename: Semsettin
authors.initials: S
authors.lastname: Sahin
mesh_terms: D016022:Case-Control Studies; D005260:Female; D005838:Genotype; D060005:Genotyping Techniques; D006801:Humans; D008297:Male; D008875:Middle Aged; D006899:Mixed Function Oxygenases / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D060888:Real-Time Polymerase Chain Reaction; D012170:Retinal Vein Occlusion / Q000201:enzymology / Q000235:genetics*; D012307:Risk Factors; D064417:Vitamin K Epoxide Reductases
chemical_list: D006899:Mixed Function Oxygenases; C578523:VKORC1 protein, human; D064417:Vitamin K Epoxide Reductases