title: Multiple cis-regulatory elements are involved in the complex regulation of the sieve element-specific MtSEO-F1 promoter from Medicago truncatula.
abstract: The sieve element occlusion (SEO) gene family includes several members that are expressed specifically in immature sieve elements (SEs) in the developing phloem of dicotyledonous plants. To determine how this restricted expression profile is achieved, we analysed the SE-specific Medicago truncatula SEO-F1 promoter (PMtSEO-F1) by constructing deletion, substitution and hybrid constructs and testing them in transgenic tobacco plants using green fluorescent protein as a reporter. This revealed four promoter regions, each containing cis-regulatory elements that activate transcription in SEs. One of these segments also contained sufficient information to suppress PMtSEO-F1 transcription in the phloem companion cells (CCs). Subsequent in silico analysis revealed several candidate cis-regulatory elements that PMtSEO-F1 shares with other SEO promoters. These putative sieve element boxes (PSE boxes) are promising candidates for cis-regulatory elements controlling the SE-specific expression of PMtSEO-F1.
authors.affiliation: Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Aachen, Germany Institut für Biologie und Biotechnologie der Pflanzen, Westfälische Wilhelms-Universität Münster, Münster, Germany Max Planck Institute for Molecular Genetics, Computational Molecular Biology, Berlin, Germany Department of Biological Sciences, University of Warwick, Coventry, UK.
authors.forename: M
authors.initials: M
authors.lastname: Bucsenez
authors.affiliation: Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Aachen, Germany Institut für Biologie und Biotechnologie der Pflanzen, Westfälische Wilhelms-Universität Münster, Münster, Germany Max Planck Institute for Molecular Genetics, Computational Molecular Biology, Berlin, Germany Department of Biological Sciences, University of Warwick, Coventry, UK.
authors.forename: B
authors.initials: B
authors.lastname: Rüping
authors.affiliation: Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Aachen, Germany Institut für Biologie und Biotechnologie der Pflanzen, Westfälische Wilhelms-Universität Münster, Münster, Germany Max Planck Institute for Molecular Genetics, Computational Molecular Biology, Berlin, Germany Department of Biological Sciences, University of Warwick, Coventry, UK.
authors.forename: S
authors.initials: S
authors.lastname: Behrens
authors.affiliation: Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Aachen, Germany Institut für Biologie und Biotechnologie der Pflanzen, Westfälische Wilhelms-Universität Münster, Münster, Germany Max Planck Institute for Molecular Genetics, Computational Molecular Biology, Berlin, Germany Department of Biological Sciences, University of Warwick, Coventry, UK.
authors.forename: R M
authors.initials: RM
authors.lastname: Twyman
authors.affiliation: Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Aachen, Germany Institut für Biologie und Biotechnologie der Pflanzen, Westfälische Wilhelms-Universität Münster, Münster, Germany Max Planck Institute for Molecular Genetics, Computational Molecular Biology, Berlin, Germany Department of Biological Sciences, University of Warwick, Coventry, UK.
authors.forename: G A
authors.initials: GA
authors.lastname: Noll
authors.affiliation: Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Aachen, Germany Institut für Biologie und Biotechnologie der Pflanzen, Westfälische Wilhelms-Universität Münster, Münster, Germany Max Planck Institute for Molecular Genetics, Computational Molecular Biology, Berlin, Germany Department of Biological Sciences, University of Warwick, Coventry, UK.
authors.forename: D
authors.initials: D
authors.lastname: Prüfer
mesh_terms: D018506:Gene Expression Regulation, Plant; D046913:Medicago truncatula / Q000235:genetics*; D016297:Mutagenesis, Site-Directed; D009928:Organ Specificity / Q000235:genetics; D052585:Phloem / Q000166:cytology / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D017384:Sequence Deletion; D016385:TATA Box / Q000235:genetics; D024363:Transcription Initiation Site
chemical_list: D010940:Plant Proteins