title: Metabolism and transportation pathways of GDP-fucose that are required for the O-fucosylation of Notch.
abstract: Notch is a single-pass transmembrane receptor that mediates the local cell-cell interactions necessary for many cell-fate decisions. The extra cellular domain of Notch contains a tandem array of epidermal growth factor-like (EGF-like) repeats. Some of these EGF-like repeats are O-fucosylated by protein O-fucosyltransferase 1 (O-fut1), which is essential for Notch signaling in Drosophila and mouse. This O-fucose is further modified by Fringe, a GlcNAc transferase and other glycosyltransferases (O-fut1 in Drosophila and Pofut1 in mouse), to form an O-linked tetrasaccharide, which modulates Notch's selective binding to its ligands.
authors.affiliation: Department of Biological Science and Technology, Tokyo University of Science, Noda, Chiba, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Yamakawa
authors.forename: Tomonori
authors.initials: T
authors.lastname: Ayukawa
authors.forename: Kenji
authors.initials: K
authors.lastname: Matsuno
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004330:Drosophila; D004815:Epidermal Growth Factor / Q000737:chemistry; D005643:Fucose / Q000737:chemistry* / Q000378:metabolism*; D016695:Glycosyltransferases / Q000378:metabolism*; D006153:Guanosine Diphosphate / Q000378:metabolism*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D051880:Receptors, Notch / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction
chemical_list: D051880:Receptors, Notch; D006153:Guanosine Diphosphate; D005643:Fucose; D004815:Epidermal Growth Factor; D016695:Glycosyltransferases