title: Effects of adipose-derived mesenchymal cells on ischemia-reperfusion injury in kidney.
abstract: BACKGROUND
  Acute kidney injury (AKI) is a critical condition for kidney and other remote organs, including the lung. However, available treatments for AKI are limited. In this study, we explored the effect of adipose-derived mesenchymal cells on a mouse model of AKI.
  
  
  METHODS
  Adipose-derived mesenchymal cells were isolated from mouse subcutaneous and peritoneal adipose tissue by digestion with collagenase type I. The left renal artery and vein of C57BL/6 mice were clamped for 45 min to induce ischemia and were injected with the adipose-derived mesenchymal cells [1 × 10(5) cells/0.2 ml phosphate-buffered saline (PBS)] or 0.2 ml PBS via the tail vein on days 0, 1, and 2.
  
  
  RESULTS
  The adipose-derived mesenchymal cells had stem-cell surface markers and multilineage differentiating potentials. Administered adipose-derived mesenchymal cells homed primarily into lung. Interestingly, repeated administration of adipose-derived mesenchymal cells reduced acute tubular necrosis and interstitial macrophage infiltration in the injured kidney, accompanied with reduced cytokine and chemokine expression.
  
  
  CONCLUSIONS
  Adipose-derived mesenchymal cells can be used as cell-based therapy for ischemic kidney injury.
authors.affiliation: Division of Blood Purification, Kanazawa University Hospital, 13-1 Takara-machi, Kanazawa 920-8641, Japan. kfuruichi@m-kanazawa.jp
authors.forename: Kengo
authors.initials: K
authors.lastname: Furuichi
authors.forename: Hidemi
authors.initials: H
authors.lastname: Shintani
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Sakai
authors.forename: Takahiro
authors.initials: T
authors.lastname: Ochiya
authors.forename: Kouji
authors.initials: K
authors.lastname: Matsushima
authors.forename: Shuichi
authors.initials: S
authors.lastname: Kaneko
authors.forename: Takashi
authors.initials: T
authors.lastname: Wada
mesh_terms: D058186:Acute Kidney Injury / Q000473:pathology / Q000628:therapy*; D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D002454:Cell Differentiation; D002469:Cell Separation; D018925:Chemokines / Q000096:biosynthesis; D016207:Cytokines / Q000096:biosynthesis; D007668:Kidney / Q000473:pathology; D007683:Kidney Tubular Necrosis, Acute / Q000517:prevention & control; D008168:Lung / Q000473:pathology; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells; D051379:Mice; D008810:Mice, Inbred C57BL; D015427:Reperfusion Injury / Q000473:pathology / Q000628:therapy*
chemical_list: D018925:Chemokines; D016207:Cytokines