title: Genetic association study between Interleukin 10 gene and dental implant loss.
abstract: OBJECTIVE
  Three single nucleotide polymorphisms (SNPs), -1082, -819 and -592, located on the promoter region of IL10 gene have been associated with high in vitro IL-10 production and autoimmune diseases. We aim to investigate whether polymorphisms in the IL10 gene would influence dental implant loss.
  
  
  METHODS
  We evaluated a total of 277 unrelated patients, including 185 individuals presenting at least one osseointegrated implant in function for six months or more and with no implant failure, and 92 individuals presenting at least one implant loss. DNA was extracted from buccal mucosa cells and SNPs were genotyped using TaqMan(®) probes-based assays.
  
  
  RESULTS
  Multiple logistic regression showed association between dental implant failure with -819(C/T) genotype (OR=3.27; 95% CI=1.02-10.46; p=0.0334). However, considering the statistical significance level α=0.004 (adjusted by Bonferroni correction of multiple comparisons), these results lost their significance. No association of dental implant loss with genotypes and alleles of the -1082 and -592 SNPs, as well as IL10 haplotypes in genotype/allele forms were found (p=0.9400; p=0.8861).
  
  
  CONCLUSIONS
  Neither the IL10 gene polymorphisms, nor haplotypes or other covariates were associated with susceptibility to dental implant failure in the studied population.
authors.affiliation: Department of Morphology, School of Dentistry at Araraquara, UNESP - São Paulo State University, Araraquara, SP, Brazil.
authors.forename: Suzane C
authors.initials: SC
authors.lastname: Pigossi
authors.forename: Fabiano
authors.initials: F
authors.lastname: Alvim-Pereira
authors.forename: Cláudia C
authors.initials: CC
authors.lastname: Montes
authors.forename: Livia S
authors.initials: LS
authors.lastname: Finoti
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Secolin
authors.forename: Paula C
authors.initials: PC
authors.lastname: Trevilatto
authors.forename: Raquel M
authors.initials: RM
authors.lastname: Scarel-Caminaga
mesh_terms: D000225:Adenine; D000483:Alleles; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use; D002318:Cardiovascular Diseases / Q000150:complications; D016022:Case-Control Studies; D003596:Cytosine; D015921:Dental Implants; D019232:Dental Restoration Failure; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006147:Guanine; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D006973:Hypertension / Q000150:complications; D016753:Interleukin-10 / Q000235:genetics*; D015810:Linkage Disequilibrium / Q000235:genetics; D008297:Male; D008875:Middle Aged; D016348:Osseointegration / Q000502:physiology; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D012216:Rheumatic Diseases / Q000150:complications; D012907:Smoking; D013941:Thymine
chemical_list: D000893:Anti-Inflammatory Agents; D000894:Anti-Inflammatory Agents, Non-Steroidal; D015921:Dental Implants; D016753:Interleukin-10; D006147:Guanine; D003596:Cytosine; D000225:Adenine; D013941:Thymine