title: Control of anti-thrombogenic properties: surface-induced self-assembly of fibrinogen fibers.
abstract: Wound healing is a complex process initiated by the formation of fibrin fibers and endothelialization. Normally, this process is triggered in a wound by thrombin cleavage of fibrinopeptides on fibrinogen molecules, which allows them to self spontaneously-assemble into large fibers that provide the support structure of the clot and promote healing. We have found that the fibrous structures can also form without thrombin on most polymer or metal surfaces, including those commonly used for stents. We show that the relatively hydrophobic E and D regions of the fibrinogen molecule are adsorbed on these surfaces, exposing the αC domains, which in turn results in the formation of large fiber structures that promote endothelial cell adhesion. We show that the entire process can be suppressed when stents or other substrates are coated with polymers that are functionalized to bind the αC domains, leading to the development of potentially nonthrombogenic implant materials.
authors.affiliation: Department of Materials Science and Engineering, State University of New York at Stony Brook, Stony Brook, New York 11794, USA. jkoo@kaeri.re.kr
authors.forename: Jaseung
authors.initials: J
authors.lastname: Koo
authors.forename: Dennis
authors.initials: D
authors.lastname: Galanakis
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Alexander
authors.initials: A
authors.lastname: Ramek
authors.forename: Adam
authors.initials: A
authors.lastname: Fields
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Ba
authors.forename: Marcia
authors.initials: M
authors.lastname: Simon
authors.forename: Miriam H
authors.initials: MH
authors.lastname: Rafailovich
mesh_terms: D000327:Adsorption; D000925:Anticoagulants / Q000138:chemical synthesis* / Q000737:chemistry; D002448:Cell Adhesion / Q000187:drug effects; D042783:Endothelial Cells / Q000187:drug effects; D005337:Fibrin / Q000138:chemical synthesis* / Q000737:chemistry*; D005340:Fibrinogen / Q000138:chemical synthesis* / Q000737:chemistry*; D010316:Particle Size; D011487:Protein Conformation; D013499:Surface Properties; D013997:Time Factors
chemical_list: D000925:Anticoagulants; D005337:Fibrin; D005340:Fibrinogen