title: MicroRNA expression profiling of carcinoma in situ cells of the testis.
abstract: Testicular germ cell tumours, seminoma (SE) and non-seminoma (NS), of young adult men develop from a precursor cell, carcinoma in situ (CIS), which resembles foetal gonocytes and retains embryonic pluripotency. We used microarrays to analyse microRNA (miRNA) expression in 12 human testis samples with CIS cells and compared it with miRNA expression profiles of normal adult testis, testis with Sertoli-cell-only that lacks germ cells, testis tumours (SE and embryonal carcinoma (EC), an undifferentiated component of NS) and foetal male and female gonads. Principal components analysis revealed distinct miRNA expression profiles characteristic for each of the different tissue types. We identified several miRNAs that were unique to testis with CIS cells, foetal gonads and testis tumours. These included miRNAs from the hsa-miR-371-373 and -302-367 clusters that have previously been reported in germ cell tumours and three miRNAs (hsa-miR-96, -141 and -200c) that were also expressed in human epididymis. We found several miRNAs that were upregulated in testis tumours: hsa-miR-9, -105 and -182-183-96 clusters were highly expressed in SE, while the hsa-miR-515-526 cluster was high in EC. We conclude that the miRNA expression profile changes during testis development and that the miRNA profile of adult testis with CIS cells shares characteristic similarities with the expression in foetal gonocytes.
authors.affiliation: Department of Growth and Reproduction, Rigshospitalet, Blegdamsvej 9, 2100 Copenhagen, Denmark.
authors.forename: Guy Wayne
authors.initials: GW
authors.lastname: Novotny
authors.forename: Kirstine C
authors.initials: KC
authors.lastname: Belling
authors.forename: Jesper Bertram
authors.initials: JB
authors.lastname: Bramsen
authors.forename: John E
authors.initials: JE
authors.lastname: Nielsen
authors.forename: Jette
authors.initials: J
authors.lastname: Bork-Jensen
authors.forename: Kristian
authors.initials: K
authors.lastname: Almstrup
authors.forename: Si Brask
authors.initials: SB
authors.lastname: Sonne
authors.forename: Jørgen
authors.initials: J
authors.lastname: Kjems
authors.forename: Ewa
authors.initials: E
authors.lastname: Rajpert-De Meyts
authors.forename: Henrik
authors.initials: H
authors.lastname: Leffers
mesh_terms: D002278:Carcinoma in Situ / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D010053:Ovary / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D013736:Testicular Neoplasms / Q000235:genetics*; D013737:Testis / Q000378:metabolism*
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger