title: Real-time PCR assay for identification of Blastomyces dermatitidis in culture and in tissue.
abstract: The TaqMan real-time PCR assay was developed from the Blastomyces dermatitidis BAD1 gene promoter. The assay identified all haplotypes of B. dermatitidis and five of six positive paraffin-embedded tissues. The assay sensitivity threshold was 1 pg genomic DNA of the mold form and 2 CFU of the yeast form of B. dermatitidis. No cross-reactivity was observed against other fungal DNA. The assay allowed rapid (5-h) identification of B. dermatitidis from culture and from clinical specimens.
authors.affiliation: Mycology Laboratory, Wadsworth Center, New York State Department of Health, Albany, New York, USA.
authors.forename: Ketevan
authors.initials: K
authors.lastname: Sidamonidze
authors.forename: Marcia K
authors.initials: MK
authors.lastname: Peck
authors.forename: Michael
authors.initials: M
authors.lastname: Perez
authors.forename: Dennis
authors.initials: D
authors.lastname: Baumgardner
authors.forename: Gerald
authors.initials: G
authors.lastname: Smith
authors.forename: Vishnu
authors.initials: V
authors.lastname: Chaturvedi
authors.forename: Sudha
authors.initials: S
authors.lastname: Chaturvedi
mesh_terms: D000818:Animals; D001758:Blastomyces / Q000302:isolation & purification*; D001759:Blastomycosis / Q000175:diagnosis* / Q000382:microbiology; D019411:Clinical Laboratory Techniques / Q000379:methods*; D004271:DNA, Fungal / Q000235:genetics; D006801:Humans; D009172:Mycology / Q000379:methods*; D057089:Pathology, Molecular / Q000379:methods*; D011401:Promoter Regions, Genetic; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D004271:DNA, Fungal