title: Fluorescent in vivo detection reveals that IgE(+) B cells are restrained by an intrinsic cell fate predisposition.
abstract: IgE antibodies may be protective in parasite immunity, but their aberrant production can lead to allergic disease and life-threatening anaphylaxis. Despite the importance of IgE regulation, few studies have directly examined the B cells that express IgE, because these cells are rare and difficult to detect. Here, we describe fluorescent IgE reporter mice and validate a flow cytometry procedure to allow sensitive and specific identification of IgE-expressing B cells in vivo. Similar to IgG1(+) cells, IgE(+) B cells differentiated into germinal center (GC) B cells and plasma cells (PCs) during primary immune responses to a T cell-dependent hapten-protein conjugate and the helminth Nippostrongylus brasiliensis. However, the participation of IgE(+) B cells in GCs was transient. IgE(+) B cells had an atypical propensity to upregulate the transcription factor Blimp-1 and undergo PC differentiation. Most IgE(+) PCs were short lived and showed reduced affinity maturation, revealing intrinsic mechanisms that restrict the IgE antibody response.
authors.affiliation: Sandler Asthma Basic Research Center, University of California, San Francisco, San Francisco, CA 94143, USA.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Yang
authors.forename: Brandon M
authors.initials: BM
authors.lastname: Sullivan
authors.forename: Christopher D C
authors.initials: CD
authors.lastname: Allen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000276:immunology; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D001483:Base Sequence; D002454:Cell Differentiation / Q000276:immunology; D002678:Chimera / Q000276:immunology / Q000378:metabolism; D005260:Female; D005455:Fluorescent Antibody Technique / Q000379:methods*; D018858:Germinal Center / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D006241:Haptens / Q000276:immunology / Q000378:metabolism; D007073:Immunoglobulin E / Q000276:immunology* / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009559:Nippostrongylus / Q000276:immunology / Q000378:metabolism; D010950:Plasma Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D000074462:Positive Regulatory Domain I-Binding Factor 1; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D014157:Transcription Factors / Q000276:immunology / Q000378:metabolism
chemical_list: D006241:Haptens; C513258:Prdm1 protein, mouse; D014157:Transcription Factors; D007073:Immunoglobulin E; D000074462:Positive Regulatory Domain I-Binding Factor 1