title: Rapid identification of Sporothrix species by T3B fingerprinting.
abstract: This article describes PCR fingerprinting using the universal primer T3B to distinguish among species of the Sporothrix complex, S. brasiliensis, S. globosa, S. mexicana, and S. schenckii. This methodology generated distinct banding patterns, allowing the correct identification of all 35 clinical isolates at the species level, confirmed by partial calmodulin (CAL) gene sequence analyses. This methodology is simple, reliable, rapid, and cheap, making it an ideal routine identification system for clinical mycology laboratories.
authors.affiliation: Laboratório de Micologia, Instituto de Pesquisa Clínica Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brazil.
authors.forename: Manoel Marques Evangelista
authors.initials: MM
authors.lastname: de Oliveira
authors.forename: Paula
authors.initials: P
authors.lastname: Sampaio
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Almeida-Paes
authors.forename: Célia
authors.initials: C
authors.lastname: Pais
authors.forename: Maria Clara
authors.initials: MC
authors.lastname: Gutierrez-Galhardo
authors.forename: Rosely Maria
authors.initials: RM
authors.lastname: Zancope-Oliveira
mesh_terms: D002147:Calmodulin / Q000235:genetics; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D058889:Molecular Typing / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D013175:Sporothrix / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D002147:Calmodulin; D017931:DNA Primers; D004271:DNA, Fungal