title: A simple fluorometric assay for DNA exonuclease activity based on graphene oxide.
abstract: A new assay platform for DNA exonuclease activity is developed based on the preferential binding of single-stranded DNA (ssDNA) over double-stranded DNA (dsDNA) to graphene oxide. This approach allows a simple and quantitative activity measurement in a short time at low cost.
authors.affiliation: Department of Chemistry, Seoul National University, Gwanak-ro, Gwanak-gu, Seoul 151-747, Republic of Korea.
authors.forename: Jieon
authors.initials: J
authors.lastname: Lee
authors.forename: Dal-Hee
authors.initials: DH
authors.lastname: Min
mesh_terms: D001483:Base Sequence; D004277:DNA, Single-Stranded / Q000235:genetics / Q000378:metabolism; D057075:Enzyme Assays / Q000379:methods*; D005090:Exodeoxyribonucleases / Q000378:metabolism*; D005470:Fluorometry / Q000379:methods*; D006108:Graphite / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry; D010087:Oxides / Q000737:chemistry*; D013379:Substrate Specificity; D013997:Time Factors
chemical_list: D004277:DNA, Single-Stranded; D010087:Oxides; D006108:Graphite; D005090:Exodeoxyribonucleases