title: The yeast nuclear pore complex and transport through it.
abstract: Exchange of macromolecules between the nucleus and cytoplasm is a key regulatory event in the expression of a cell's genome. This exchange requires a dedicated transport system: (1) nuclear pore complexes (NPCs), embedded in the nuclear envelope and composed of proteins termed nucleoporins (or "Nups"), and (2) nuclear transport factors that recognize the cargoes to be transported and ferry them across the NPCs. This transport is regulated at multiple levels, and the NPC itself also plays a key regulatory role in gene expression by influencing nuclear architecture and acting as a point of control for various nuclear processes. Here we summarize how the yeast Saccharomyces has been used extensively as a model system to understand the fundamental and highly conserved features of this transport system, revealing the structure and function of the NPC; the NPC's role in the regulation of gene expression; and the interactions of transport factors with their cargoes, regulatory factors, and specific nucleoporins.
authors.affiliation: Institute for Systems Biology and Seattle Biomedical Research Institute, Seattle, Washington 98109, USA. jaitchison@systemsbiology.org
authors.forename: John D
authors.initials: JD
authors.lastname: Aitchison
authors.forename: Michael P
authors.initials: MP
authors.lastname: Rout
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000502:physiology*; D001692:Biological Transport; D003599:Cytoskeleton / Q000378:metabolism; D005998:Glycine / Q000737:chemistry; D028884:Karyopherins / Q000378:metabolism; D022022:Nuclear Pore / Q000737:chemistry / Q000502:physiology*; D028861:Nuclear Pore Complex Proteins / Q000378:metabolism; D010649:Phenylalanine / Q000737:chemistry; D011485:Protein Binding; D055503:Protein Multimerization; D034443:RNA Transport / Q000502:physiology; D015003:Yeasts / Q000502:physiology*
chemical_list: D028884:Karyopherins; D028861:Nuclear Pore Complex Proteins; D010649:Phenylalanine; D005998:Glycine