title: Two-dimensional thin layer chromatographic separation of phenolic compounds from Eupatorium cannabinum extracts and their antioxidant activity.
abstract: Two dimensional thin layer chromatography on DIOL polar bonded stationary phase was performed to optimize the separation of some anioxidant phenolic compounds from Eupatorium cannabinum extracts. Ethyl-methyl ketone mixed with n-heptane and ethyl acetate mixed with n-heptane were used as non-aqueous mobile phases in normal phase separations (1st direction of development in 2D-HPTLC mode) and methanol mixed with water was used as a mobile phase in reversed phase (2nd direction of development in 2D-HPTLC mode). The plates were sprayed by use of Merck TLC sprayer using 2-(diphenylboryoxy)-ethylamine and PEG4000 (Merck, Darmstadt, Germany) or DPPH and photographed in Camag Cabinet UV lamp at 254 nm and 365 nm by use of Fuji 8 mpx camera. Satisfactory separations of antioxidant phenolic compounds in Eupatorium cannabinum extracts were obtained by use of optimized 2D-HPTLC systems.
authors.affiliation: Department of Inorganic Chemistry, Faculty of Pharmacy, Medical University of Lublin, Lublin, Poland. mirek.hawryl@umlub.pl
authors.forename: Miroslaw A
authors.initials: MA
authors.lastname: Hawrył
authors.forename: Renata
authors.initials: R
authors.lastname: Nowak
authors.forename: Monika
authors.initials: M
authors.lastname: Waksmundzka-Hajnos
authors.forename: Ryszard
authors.initials: R
authors.lastname: Swieboda
authors.forename: Monika
authors.initials: M
authors.lastname: Robak
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002855:Chromatography, Thin Layer; D036602:Eupatorium / Q000737:chemistry*; D035264:Flowers / Q000737:chemistry; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D010636:Phenols; D010936:Plant Extracts