title: Effect of dentin pre-treatment with a casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) paste on dentin bond strength in tridimensional cavities.
abstract: OBJECTIVE
  This study aimed to evaluate the push-out bond strength of dimethacrylate (Clearfil SE Bond/Filtek Z250; and Adper SE Plus/Filtek Z250) and silorane-based (Filtek P90 adhesive system/Filtek P90 composite resin) restorative systems following selective dentin pre-treatment with a CPP-ACP-containing paste (MI Paste).
  
  
  METHODS
  Sixty bovine incisors were utilized. The buccal surface was wet-ground to obtain a flat dentin area. Standardized conical cavities were then prepared. Adhesive systems were applied according to manufacturers' directions and the composites were bulk-inserted into the cavity. The push-out bond strength test was performed at a universal testing machine (0.5 mm/min) until failure; failure modes were analyzed by scanning electron microscopy. Data were analyzed by two-way ANOVA and Tukey post-hoc test (p < 0.05).
  
  
  RESULTS
  For Clearfil SE Bond/Filtek Z250 and Filtek P90 adhesive system/Filtek P90 composite resin, the dentin pre-treatment did not influence bond strength means. For Adper SE Plus/Filtek Z250, dentin samples treated with MI Paste had statistically higher bond strength means than non-treated specimens. Adhesive failures were more frequent.
  
  
  CONCLUSIONS
  Dentin pre-treatment with the CPP-ACP containing paste did not negatively affect bond strength for Clearfil SE Bond/Filtek Z250 and Filtek P90 adhesive system/Filtek P90 composite resin restorative systems and improved bond strength for the Adper SE Plus/Filtek Z250 restorative system.
authors.affiliation: Department of Dentistry, School of Dentistry, Potiguar University, Laureate International Universities, Natal, Brazil. boniek.castillo@gmail.com
authors.forename: Boniek Castillo Dutra
authors.initials: BC
authors.lastname: Borges
authors.forename: Eduardo Jose
authors.initials: EJ
authors.lastname: Souza-Junior
authors.forename: Giovanna de Fátima Alves
authors.initials: Gde F
authors.lastname: da Costa
authors.forename: Isauremi Vieira de Assunçao
authors.initials: IV
authors.lastname: Pinheiro
authors.forename: Mário Alexandre Coelho
authors.initials: MA
authors.lastname: Sinhoreti
authors.forename: Rodivan
authors.initials: R
authors.lastname: Braz
authors.forename: Marcos Antônio Japiassú Resende
authors.initials: MA
authors.lastname: Montes
mesh_terms: D000818:Animals; D002327:Cariostatic Agents / Q000494:pharmacology*; D002364:Caseins / Q000494:pharmacology*; D002417:Cattle; D003188:Composite Resins; D001840:Dental Bonding; D003737:Dental Cavity Preparation; D018772:Dental Marginal Adaptation; D003793:Dental Restoration, Permanent; D003799:Dental Stress Analysis; D003804:Dentin / Q000187:drug effects*; D017220:Dentin-Bonding Agents; D019279:Resin Cements; D062047:Silorane Resins
chemical_list: D002327:Cariostatic Agents; D002364:Caseins; D003188:Composite Resins; D017220:Dentin-Bonding Agents; D019279:Resin Cements; D062047:Silorane Resins; C447032:casein phosphopeptide-amorphous calcium phosphate nanocomplex