title: Nitric oxide-releasing nanoparticles accelerate wound healing in NOD-SCID mice.
abstract: Wound healing is a complex process, coordinated by various biological factors. In immunocompromised states wound healing can be interrupted as a result of decreased numbers of immune cells, impairing the production of effector molecules such as nitric oxide (NO). Therefore, topical NO-releasing platforms, such as diethylenetriamine (DETA NONOate), have been investigated to enhance wound healing. Recently, we demonstrated a nanoparticle platform that releases NO (NO-NPs) in a sustained manner, accelerating wound healing in both uninfected and infected murine wound models. Here, NO-NPs were investigated and compared to DETA NONOate in an immunocompromised wound model using non-obese, diabetic, severe combined immunodeficiency mice. NO-NP treatment accelerated wound closure as compared to controls and DETA NONOate treatment. In addition, histological assessment revealed that wounds treated with NO-NPs had less inflammation, more collagen deposition, and more blood vessel formation as compared to other groups, consistent with our previous data in immunocompetent animals. These data suggest that NO-NPs may serve as a novel wound-healing therapy in the setting of immunocompromised states associated with impaired wound healing.
  
  
  BACKGROUND
  Wound healing in an immunocompromised host is often incomplete and is a source of major concern in such conditions. This work demonstrates in a murine model that in these settings NO releasing nanoparticles significantly enhance wound healing.
authors.affiliation: Department of Medicine (Division of Dermatology) Montefiore Medical Center, Bronx, New York 10467, USA.
authors.forename: Karin
authors.initials: K
authors.lastname: Blecher
authors.forename: Luis R
authors.initials: LR
authors.lastname: Martinez
authors.forename: Chaim
authors.initials: C
authors.lastname: Tuckman-Vernon
authors.forename: Parimala
authors.initials: P
authors.lastname: Nacharaju
authors.forename: David
authors.initials: D
authors.lastname: Schairer
authors.forename: Jason
authors.initials: J
authors.lastname: Chouake
authors.forename: Joel M
authors.initials: JM
authors.lastname: Friedman
authors.forename: Alan
authors.initials: A
authors.lastname: Alfieri
authors.forename: Chandan
authors.initials: C
authors.lastname: Guha
authors.forename: Joshua D
authors.initials: JD
authors.lastname: Nosanchuk
authors.forename: Adam J
authors.initials: AJ
authors.lastname: Friedman
mesh_terms: D000818:Animals; D003094:Collagen / Q000378:metabolism; D005260:Female; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry; D009569:Nitric Oxide / Q000008:administration & dosage / Q000737:chemistry; D010974:Platelet Aggregation / Q000187:drug effects; D012867:Skin / Q000187:drug effects / Q000473:pathology; D014945:Wound Healing / Q000187:drug effects*; D014946:Wound Infection / Q000188:drug therapy / Q000473:pathology
chemical_list: D009569:Nitric Oxide; D003094:Collagen