title: Photo-induced proton gradients for the in vitro investigation of bacterial efflux pumps.
abstract: We describe an original activity assay for membrane transport that uses the proton motive force-dependent efflux pump MexAB from Pseudomonas aeruginosa. This pump is co-reconstituted into proteoliposomes together with bacteriorhodopsin (BR), a light-activated proton pump. In this system, upon illumination with visible light, the photo-induced proton gradient created by the BR is shown to be coupled to the active transport of substrates through the pump.
authors.affiliation: Laboratoire de Cristallographie et RMN Biologiques (CNRS UMR8015), Faculté de Pharmacie, Université Paris Descartes , Sorbonne Paris Cité, 4 Avenue de l'Observatoire, 75006 Paris,  France.
authors.forename: Alice
authors.initials: A
authors.lastname: Verchère
authors.forename: Isabelle
authors.initials: I
authors.lastname: Broutin
authors.forename: Martin
authors.initials: M
authors.lastname: Picard
mesh_terms: D001692:Biological Transport; D066298:In Vitro Techniques; D055668:Photochemical Processes; D011522:Protons; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
chemical_list: D011522:Protons