title: Microchip capillary gel electrophoresis of multiply PEGylated high-molecular-mass glycoproteins.
abstract: PEGylation is the most successful approach, to date, to prolong the in vivo survival of recombinant proteins. The conjugation of the polymer to glycoproteins results in challenging analysis, and furthermore, requires a wide variety of analytical tools for the determination of the extent of PEGylation. Herein, we present microchip capillary gel electrophoresis (MCGE) with a non-commercial high-molecular-weight protein assay for the analysis of the PEGylation degree with a focus on multiple PEGylation. To show the potential of the modified MCGE system, high-mass PEGylated glycoproteins (e.g. coagulation factor VIII) were analyzed. For the von Willebrand factor, the influence of glycans and the hydrodynamic radius on migration time and molecular weight determination is shown. The modified MCGE assay system is a powerful tool for the rapid assessment of the degree of PEGylation, demonstrating conjugate quality or reaction control of PEGylated proteins. This is the main advantage over time-consuming conventional SDS-PAGE. Furthermore, electrophoretic separation, staining, destaining, and fluorescence detection in one step combined with automated data analysis show that the MCGE system is a promising technique for high-throughput monitoring. The MCGE system can be used for rapid structure confirmation ("MCGE fingerprinting") of multiply PEGylated glycoproteins beyond the 230 kDa molecular mass range.
authors.affiliation: Institute of Chemical Technologies and Analytics, Vienna University of Technology, Getreidemarkt 9/164, Vienna, Austria.
authors.forename: Birgit K
authors.initials: BK
authors.lastname: Seyfried
authors.forename: Martina
authors.initials: M
authors.lastname: Marchetti-Deschmann
authors.forename: Jürgen
authors.initials: J
authors.lastname: Siekmann
authors.forename: Mary J
authors.initials: MJ
authors.lastname: Bossard
authors.forename: Friedrich
authors.initials: F
authors.lastname: Scheiflinger
authors.forename: Peter L
authors.initials: PL
authors.lastname: Turecek
authors.forename: Günter
authors.initials: G
authors.lastname: Allmaier
mesh_terms: D046209:Electrophoresis, Microchip / Q000379:methods*; D005169:Factor VIII / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D006023:Glycoproteins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D008970:Molecular Weight; D011092:Polyethylene Glycols / Q000737:chemistry*; D011994:Recombinant Proteins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D012709:Serum Albumin / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D014841:von Willebrand Factor / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D006023:Glycoproteins; D011994:Recombinant Proteins; D012709:Serum Albumin; D014841:von Willebrand Factor; D011092:Polyethylene Glycols; D005169:Factor VIII