title: A study of electrochemical biosensor for analysis of three-dimensional (3D) cell culture.
abstract: Cell culture has a fundamental role not only in regenerative medicine but also in biotechnology, pharmacology, impacting both drug discovery and manufacturing. Although cell culture has been generally developed for only two-dimensional (2D) culture systems, three-dimensional (3D) culture is being spotlighted as the means to mimic in vivo cellular conditions. In this study, a method for cytotoxicity assay using an electrochemical biosensor applying 3D cell culture is presented. In order to strengthen the advantage of a 3D cell culture, the experimental condition of gelation between several types of sol-gels (alginate, collagen, matrigel) and cancer cells can be optimized to make a 3D cell structure on the electrode, which will show the reproducibility of electrical measurement for long-term monitoring. Moreover, cytotoxicity test results applying this method showed IC(50) value of A549 lung cancer cells to erlotinib. Thus, this study evaluates the feasibility of application of the electrochemical biosensor for 3D cell culture to cytotoxicity assay for investigation of 3D cell response to drug compounds.
authors.affiliation: Advanced Materials & Devices Lab, Corporate R&D Institute, Samsung Electro-Mechanics Co., Ltd., Suwon 443-743, Republic of Korea. Electronic address: sehoon.jeong@samsung.com.
authors.forename: Se Hoon
authors.initials: SH
authors.lastname: Jeong
authors.affiliation: Advanced Materials & Devices Lab, Corporate R&D Institute, Samsung Electro-Mechanics Co., Ltd., Suwon 443-743, Republic of Korea.
authors.forename: Dong Woo
authors.initials: DW
authors.lastname: Lee
authors.affiliation: College of Bionano technology, Gachon University, Seongnam 461-701, Republic of Korea.
authors.forename: Sanghyo
authors.initials: S
authors.lastname: Kim
authors.affiliation: Samsung Medical Center, School of Medicine, Sungkyunkwan University, Seoul 135-230, Republic of Korea.
authors.forename: Jhingook
authors.initials: J
authors.lastname: Kim
authors.affiliation: Advanced Materials & Devices Lab, Corporate R&D Institute, Samsung Electro-Mechanics Co., Ltd., Suwon 443-743, Republic of Korea. Electronic address: bosung.ku@samsung.com.
authors.forename: Bosung
authors.initials: B
authors.lastname: Ku
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage; D015374:Biosensing Techniques / Q000379:methods*; D002452:Cell Count; D018929:Cell Culture Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D002470:Cell Survival; D003094:Collagen; D004338:Drug Combinations; D055664:Electrochemical Techniques; D000069347:Erlotinib Hydrochloride; D005782:Gels; D006801:Humans; D007797:Laminin; D008175:Lung Neoplasms / Q000188:drug therapy / Q000473:pathology; D011509:Proteoglycans; D011799:Quinazolines / Q000008:administration & dosage; D018874:Spheroids, Cellular / Q000166:cytology / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; D004338:Drug Combinations; D005782:Gels; D007797:Laminin; D011509:Proteoglycans; D011799:Quinazolines; C059485:matrigel; D003094:Collagen; D000069347:Erlotinib Hydrochloride