title: Amperometric biosensor based on diamond paste for the enantioanalysis of L-lysine.
abstract: An amperometric biosensor was proposed for the enantioanalysis of L-lysine. The biosensor is based on the impregnation of L-lysine oxidase in diamond paste. The potential used for the determination of l-lysine was 650 mV. The biosensor exhibited a linear concentration range between 1 and 100 nmol/L with a limit of detection of 4 pmol/L. The selectivity of the biosensor is high over other amino acids, such as L-serine, L-leucine, L-aspartic acid, L-glutamic acid, histamine, glycine. The proposed biosensor can be applied for the determination of L-lysine in serum samples and pharmaceutical compounds.
authors.affiliation: Laboratory of Electrochemistry and PATLAB Bucharest, National Institute of Research for Electrochemistry and Condensed Matter, 202 Splaiul Independentei Str., 060021, Bucharest 6, Romania. Electronic address: iustinavanstaden@yahoo.com.
authors.forename: Raluca-Ioana
authors.initials: RI
authors.lastname: Stefan-van Staden
authors.affiliation: Department of Chemistry, Al-Aqsa University, Gaza, Palestine.
authors.forename: R'afat Mahmoud
authors.initials: RM
authors.lastname: Nejem
authors.affiliation: Laboratory of Electrochemistry and PATLAB Bucharest, National Institute of Research for Electrochemistry and Condensed Matter, 202 Splaiul Independentei Str., 060021, Bucharest 6, Romania.
authors.forename: Jacobus Frederick
authors.initials: JF
authors.lastname: van Staden
authors.affiliation: Pharmaceutical and Medicinal Department, Pharmaceutical and Drug Industries Research Divison, National Research Centre (NRC), Dokki, Cairo 12311, Egypt.
authors.forename: Hassan Y
authors.initials: HY
authors.lastname: Aboul-Enein
mesh_terms: D000594:Amino Acid Oxidoreductases; D015374:Biosensing Techniques / Q000379:methods* / Q000706:statistics & numerical data; D001774:Blood Chemical Analysis / Q000379:methods / Q000706:statistics & numerical data; D018130:Diamond; D055664:Electrochemical Techniques; D004800:Enzymes, Immobilized; D006801:Humans; D057230:Limit of Detection; D008239:Lysine / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D013237:Stereoisomerism
chemical_list: D004800:Enzymes, Immobilized; D018130:Diamond; D000594:Amino Acid Oxidoreductases; C041123:L-lysine oxidase; D008239:Lysine