title: [The expression and significance of TLR4, MyD88 and NF-κB mRNA in mouse lymph node of experimental autoimmune myositis].
abstract: OBJECTIVE
  To investigate TLR4, MyD88 and NF-κB mRNA levels in mouse lymph node with experimental autoimmune myositis(EAM)and determine the role of TLR4 in autoimmune myositis.
  
  
  METHODS
  Thirty femal BALB/c mice were randomly divided into five groups (n=6  animals per group): group 1 was the control,  while animals in other four groups were killed at different time point: group 2 in the first week,  group 3 in the second week,  group 4 in the third week and group 5 in the fourth week since they had been given myosin for preparing EAM. The expressions of TLR4, MyD88 and NF-κB mRNA were measured with real-time fluorescent quantitative polymerase chain reaction.
  
  
  RESULTS
  (1)The expressions of TLR4, MyD88 and NF-κB mRNA in each EAM group were significantly high compared with those in the normal control group,  which was significantly highest in group 3 of all(P<0.01) and significantly higher in group 4 than in group 5(P<0.01).(2)The expression level of TLR4 mRNA had significant positive correlations with the expressions of MyD88 mRNA and NF-κB mRNA(r=0.906, r=0.967,  P<0.01),  and the latter two also had significant positive correlations(r=0.919, P<0.01).
  
  
  CONCLUSIONS
  TLR4 played an important role in the development of autoimmune myositis and run its function mainly by MyD88-dependent pathway that could activate NF-κB for promoting the release of inflammatory factors.
authors.affiliation: Department of Neurology, Xijing Hospital, Fourth Military Medical University, Xi'an, China.
authors.forename: Hong-ya
authors.initials: HY
authors.lastname: Zhang
authors.forename: Juan
authors.initials: J
authors.lastname: Kang
authors.forename: Wen-juan
authors.initials: WJ
authors.lastname: Han
authors.forename: Meng-meng
authors.initials: MM
authors.lastname: Hu
authors.forename: Hong-ge
authors.initials: HG
authors.lastname: Jia
mesh_terms: D000818:Animals; D005260:Female; D016014:Linear Models; D008198:Lymph Nodes / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D053594:Myeloid Differentiation Factor 88 / Q000235:genetics*; D016328:NF-kappa B / Q000235:genetics*; D020721:Nervous System Autoimmune Disease, Experimental / Q000235:genetics* / Q000276:immunology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000235:genetics*
chemical_list: D053594:Myeloid Differentiation Factor 88; D016328:NF-kappa B; D012333:RNA, Messenger; C493487:Tlr4 protein, mouse; D051197:Toll-Like Receptor 4