title: A new loop-mediated isothermal amplification method for rapid, simple, and sensitive detection of Leptospira spp. in urine.
abstract: We developed a new loop-mediated isothermal amplification (LAMP) method to detect rrs, a 16S rRNA gene of pathogenic Leptospira spp. in urine. The method enables detection of two leptospiral cells per reaction mixture following boiling of urine specimens. The sensitivity of this method is higher than that of culture or of flaB nested PCR.
authors.affiliation: Department of Bacteriology, National Institute of Infectious Disease, Tokyo, Japan. nkoizumi@nih.go.jp
authors.forename: Nobuo
authors.initials: N
authors.lastname: Koizumi
authors.forename: Chie
authors.initials: C
authors.lastname: Nakajima
authors.forename: Tsunehito
authors.initials: T
authors.lastname: Harunari
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Tanikawa
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Tokiwa
authors.forename: Eriko
authors.initials: E
authors.lastname: Uchimura
authors.forename: Tokujiro
authors.initials: T
authors.lastname: Furuya
authors.forename: Claro Niegos
authors.initials: CN
authors.lastname: Mingala
authors.forename: Marvin Ardeza
authors.initials: MA
authors.lastname: Villanueva
authors.forename: Makoto
authors.initials: M
authors.lastname: Ohnishi
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Suzuki
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D020459:Genes, rRNA; D006801:Humans; D007919:Leptospira / Q000302:isolation & purification*; D007922:Leptospirosis / Q000175:diagnosis* / Q000382:microbiology*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods; D013696:Temperature; D014556:Urine / Q000382:microbiology*
chemical_list: D012336:RNA, Ribosomal, 16S