title: Antitumor activity of an interleukin-2 monoclonal antibody in a murine osteosarcoma transplantation model.
abstract: BACKGROUND
  Injection of monoclonal antibody to interleukin-2 (S4B6) into mice depletes regulatory T-cells (Tregs) and exhibits antitumor activities mediated through an autoimmune reaction. In this study, we demonstrate that administration of S4B6 suppresses the murine osteosarcoma cell line, LM8.
  
  
  METHODS
  LM8 osteosarcoma cells were transplanted subcutaneously into C3H mice (n=58). C3H mice were injected intraperitoneally with S4B6 starting at 7 days before LM8 transplantation (pre-S4B6 group), 2 days after transplantation, or 5 days after transplantation. Control group mice were injected with normal rat IgG. Mice were sacrificed and examined 4 weeks later.
  
  
  RESULTS
  The number of pulmonary metastatic colonies and the tumor size were significantly reduced in the pre-S4B6 group compared to the control group. In addition, pulmonary metastases were inhibited in mice injected with S4B6 2 days, but not 5 days, after tumor transplantation.
  
  
  CONCLUSIONS
  S4B6 administration inhibited metastasis even when injected 2 days after LM8 transplantation. Our data suggest that treatment with S4B6 might be suitable in a postoperative clinical setting.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Aichi Cancer Center Hospital, Chikusa-ku, Nagoya, Japan. casey_cybercat@yahoo.co.jp
authors.forename: Keishi
authors.initials: K
authors.lastname: Kohyama
authors.forename: Hideshi
authors.initials: H
authors.lastname: Sugiura
authors.forename: Eiji
authors.initials: E
authors.lastname: Kozawa
authors.forename: Junji
authors.initials: J
authors.lastname: Wasa
authors.forename: Kenji
authors.initials: K
authors.lastname: Yamada
authors.forename: Akiko
authors.initials: A
authors.lastname: Nishioka
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Kamei
authors.forename: Osamu
authors.initials: O
authors.lastname: Taguchi
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000627:therapeutic use*; D004195:Disease Models, Animal; D007376:Interleukin-2 / Q000276:immunology*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D009368:Neoplasm Transplantation; D012516:Osteosarcoma / Q000276:immunology / Q000628:therapy*
chemical_list: D000911:Antibodies, Monoclonal; D007376:Interleukin-2