title: A unique MUC1-2-VNTR DNA vaccine suppresses tumor growth and prolongs             survival in a murine multiple myeloma model.
abstract: Multiple myeloma (MM) is a clonal B-cell malignancy charactered by the aberrant             proliferation of malignant plasma cells in the bone marrow. MM is still an incurable             malignancy. In this regard, novel treatments are urgently required. MUC1 (mucin             1), a type І transmembrane protein, is overexpressed and aberrantly glycosylated             in many carcinomas particularly in MM resulting in an antigenically distinct molecule             and may be a potential target for specific immunotherapy. In this study, we first             designed a unique DNA vaccine, termed MUC1-2-VNTR (various number tandem repeats)             to investigate whether the vaccine could specifically suppress tumor growth in             a murine multiple myloma model. Our results showed that the constructed DNA vaccine             pcDNA3.1-VNTR elicited both humoral and cellular tumor-specific immune responses             in the MM mouse model leading to delay in tumor growth and prolonged survival             of the mice. Consequently, our study indicates that this DNA vaccine shows promise             to be used as a novel strategy for the treatment of MM.
authors.affiliation: Department of Hematology, Second affiliated Hospital of Kunming Medical University, Kunming 650101, PR China.
authors.forename: Yuhui
authors.initials: Y
authors.lastname: Weng
authors.forename: Liang
authors.initials: L
authors.lastname: Shao
authors.forename: Hongmei
authors.initials: H
authors.lastname: Ouyang
authors.forename: Yuebo
authors.initials: Y
authors.lastname: Liu
authors.forename: Jin
authors.initials: J
authors.lastname: Yao
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.forename: Yunjiao
authors.initials: Y
authors.lastname: Luo
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Zhao
authors.forename: Hong
authors.initials: H
authors.lastname: Mou
authors.forename: Zeping
authors.initials: Z
authors.lastname: Zhou
authors.forename: You
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D019556:COS Cells; D019496:Cancer Vaccines / Q000235:genetics / Q000276:immunology / Q000627:therapeutic use*; D002522:Chlorocebus aethiops; D004195:Disease Models, Animal; D005260:Female; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D018598:Minisatellite Repeats; D018396:Mucin-1 / Q000235:genetics* / Q000276:immunology; D009101:Multiple Myeloma / Q000276:immunology / Q000628:therapy*; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology / Q000627:therapeutic use*
chemical_list: D019496:Cancer Vaccines; D007074:Immunoglobulin G; D018396:Mucin-1; D019444:Vaccines, DNA