title: Expression of the newly identified gene CAC1 in the hippocampus of Alzheimer's disease patients.
abstract: Alzheimer's disease (AD), the most common form of senile dementia, is associated with neurodegeneration. The development of Alzheimer's disease is related to abnormalities of cell cycle regulation. Preliminary work showed that a novel gene, CAC1, was highly expressed in tumors and had an oncogene-like function related to cell cycle regulation. The pathogenesis of AD is still incompletely understood. In this study, we measured the expression level of CAC1 in the hippocampus of AD patients to explore the involvement of CAC1 in the development of AD. Our findings showed that the expression level of CAC1 in the hippocampus of AD patients was significantly lower than that of normal controls. The reduction of CAC1 expression did not affect tau/p-tau-396, amyloid precursor protein or apolipoprotein E4 in the in vitro model. A reduction of cyclin E was detected after a CAC1-knockdown. Interestingly, we found that the knockdown of CAC1 by RNAi led to an increase in oxidative stress and the level of p53 protein in SHSY-5Y cells. The expression of CAC1 in SHSY-5Y cells protected the cells from apoptosis induced by Aβ toxicity or oxidative stress. These results established that CAC1 is an important factor for the protection of cells against Aβ toxicity and oxidative stress.
authors.affiliation: Department of Oncology, First Hospital of Xi'an Jiaotong University, No. 277 YanTa West Road, Xi'an, Shaanxi, 710061, People's Republic of China. yingkong76@gmail.com
authors.forename: Ying
authors.initials: Y
authors.lastname: Kong
authors.forename: Pei-song
authors.initials: PS
authors.lastname: Bai
authors.forename: Hong
authors.initials: H
authors.lastname: Sun
authors.forename: Ke-jun
authors.initials: KJ
authors.lastname: Nan
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D017209:Apoptosis / Q000502:physiology*; D045744:Cell Line, Tumor; D044842:Cullin Proteins / Q000096:biosynthesis / Q000235:genetics*; D005260:Female; D006367:HeLa Cells; D006624:Hippocampus / Q000378:metabolism / Q000473:pathology / Q000502:physiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D018696:Neuroprotective Agents / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology
chemical_list: C546534:CACUL1 protein, human; D044842:Cullin Proteins; D018696:Neuroprotective Agents