title: Effect of heat shock on ultrastructure and calcium distribution in Lavandula pinnata L. glandular trichomes.
abstract: The effects of heat shock (HS) on the ultrastructure and calcium distribution of Lavandula pinnata secretory trichomes are examined using transmission electron microscopy and potassium antimonate precipitation. After 48-h HS at 40°C, plastids become distorted and lack stroma and osmiophilic deposits, the cristae of the mitochondria become indistinct, the endoplasmic reticulum acquires a chain-like appearance with ribosomes prominently attached to the lamellae, and the plasma and organelle membranes become distorted. Heat shock is associated with a decrease in calcium precipitates in the trichomes, while the number of precipitates increases in the mesophyll cells. Prolonged exposure to elevated calcium levels may be toxic to the mesophyll cells, while the lack of calcium in the glands cell may deprive them of the normal protective advantages of elevated calcium levels. The inequality in calcium distribution may result not only from uptake from the transpiration stream, but also from redistribution of calcium from the trichomes to the mesophyll cells.
authors.affiliation: Guangdong Research Institute of Traditional Chinese Medicine, Guangzhou 510520, China. hss@scib.ac.cn
authors.forename: S S
authors.initials: SS
authors.lastname: Huang
authors.forename: B K
authors.initials: BK
authors.lastname: Kirchoff
authors.forename: J P
authors.initials: JP
authors.lastname: Liao
mesh_terms: D002118:Calcium / Q000378:metabolism*; D004533:Egtazic Acid / Q000494:pharmacology; D006360:Heat-Shock Proteins / Q000378:metabolism; D018869:Heat-Shock Response / Q000502:physiology*; D027523:Lavandula / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure*; D010940:Plant Proteins / Q000378:metabolism; D064201:Trichomes / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D006360:Heat-Shock Proteins; D010940:Plant Proteins; D004533:Egtazic Acid; D002118:Calcium