title: Methods for comprehensive chromosome screening of oocytes and embryos: capabilities, limitations, and evidence of validity.
abstract: Preimplantation aneuploidy screening of cleavage stage embryos using fluorescence in situ hybridization (FISH) may no longer be considered the standard of care in reproductive medicine. Over the last few years, there has been considerable development of novel technologies for comprehensive chromosome screening (CCS) of the human genome. Among the notable methodologies that have been incorporated are whole genome amplification, metaphase and array based comparative genomic hybridization, single nucleotide polymorphism microarrays, and quantitative real-time PCR. As these methods become more integral to treating patients with infertility, it is critical that clinicians and scientists obtain a better understanding of their capabilities and limitations. This article will focus on reviewing these technologies and the evidence of their validity.
authors.affiliation: Reproductive Medicine Associates of New Jersey, Morristown, NJ 07960, USA. ntreff@rmanj.com
authors.forename: Nathan R
authors.initials: NR
authors.lastname: Treff
authors.forename: Richard T
authors.initials: RT
authors.lastname: Scott
mesh_terms: D055028:Comparative Genomic Hybridization / Q000379:methods / Q000592:standards; D005260:Female; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods / Q000592:standards; D009865:Oocytes / Q000502:physiology*; D019836:Preimplantation Diagnosis / Q000379:methods* / Q000592:standards; D015203:Reproducibility of Results