title: Inhibition of hepatitis B virus gene expression by small interfering RNAs targeting cccDNA and X antigen.
abstract: To test the possible inhibition of hepatitis B virus (HBV) replication and expression by small interfering RNAs (siRNAs) targeting simultaneously covalenthy closed circular DNA (dnacccDNA) and X antigen, corresponding recombinant plasmids were transfected into HepG2.2.15 cells and the levels of cccDNA, HBXAg, HBcAg, and HBeAg were assayed at various times post transfection. As expected, the single siRNAs showed marked inhibitory effects but their combination was even more efficient. These results provide a new insight into the development of a potential anti-HBV strategy of enhancing the efficacy of individual antivirals and overcoming the high mutation rate of HBV.
authors.affiliation: Sichuan University, Chengdu, People's Republic of China.
authors.forename: Q
authors.initials: Q
authors.lastname: Xie
authors.forename: S
authors.initials: S
authors.lastname: Zhang
authors.forename: W
authors.initials: W
authors.lastname: Wang
authors.forename: Y M
authors.initials: YM
authors.lastname: Li
authors.forename: T
authors.initials: T
authors.lastname: Du
authors.forename: X L
authors.initials: XL
authors.lastname: Su
authors.forename: Y Q
authors.initials: YQ
authors.lastname: Wei
authors.forename: H X
authors.initials: HX
authors.lastname: Deng
mesh_terms: D004270:DNA, Circular / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D015870:Gene Expression; D056945:Hep G2 Cells; D006512:Hepatitis B Core Antigens / Q000096:biosynthesis / Q000235:genetics; D006514:Hepatitis B Surface Antigens / Q000096:biosynthesis / Q000235:genetics; D006513:Hepatitis B e Antigens / Q000096:biosynthesis / Q000235:genetics; D006515:Hepatitis B virus / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D010957:Plasmids; D034741:RNA, Small Interfering / Q000235:genetics*; D014162:Transfection; D014764:Viral Proteins / Q000235:genetics*; D014779:Virus Replication / Q000235:genetics
chemical_list: D004270:DNA, Circular; D004279:DNA, Viral; D006512:Hepatitis B Core Antigens; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; D034741:RNA, Small Interfering; D014764:Viral Proteins