title: Dynamics of subcellular proteomes during brain development.
abstract: Many neurological disorders are caused by perturbations during brain development, but these perturbations cannot be readily identified until there is comprehensive description of the development process. In this study, we performed mass spectrometry analysis of the synaptosomal and mitochondrial fractions from three rat brain regions at four postnatal time points. To quantitate our analysis, we employed (15)N labeled rat brains using a technique called SILAM (stable isotope labeling in mammals). We quantified 167429 peptides and identified over 5000 statistically significant changes during development including known disease-associated proteins. Global analysis revealed distinct trends between the synaptic and nonsynaptic mitochondrial proteomes and common protein networks between regions each consisting of a unique array of expression patterns. Finally, we identified novel regulators of neurodevelopment that possess the identical temporal pattern of known regulators of neurodevelopment. Overall, this study is the most comprehensive quantitative analysis of the developing brain proteome to date, providing an important resource for neurobiologists.
authors.affiliation: The Scripps Research Institute, La Jolla, California 92037, United States.
authors.forename: Daniel B
authors.initials: DB
authors.lastname: McClatchy
authors.forename: Lujian
authors.initials: L
authors.lastname: Liao
authors.forename: Ji Hyoung
authors.initials: JH
authors.lastname: Lee
authors.forename: Sung Kyu
authors.initials: SK
authors.lastname: Park
authors.forename: John R
authors.initials: JR
authors.lastname: Yates
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001921:Brain / Q000254:growth & development* / Q000378:metabolism*; D001923:Brain Chemistry / Q000502:physiology*; D042541:Intracellular Space / Q000737:chemistry / Q000378:metabolism; D007553:Isotope Labeling; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000378:metabolism*; D009587:Nitrogen Isotopes / Q000032:analysis / Q000378:metabolism; D060066:Protein Interaction Maps; D020543:Proteome / Q000032:analysis / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D009419:Nerve Tissue Proteins; D009587:Nitrogen Isotopes; D020543:Proteome