title: A set of host proteins interacting with papaya ringspot virus NIa-Pro protein identified in a yeast two-hybrid system.
abstract: The protein-protein interactions between viral and host proteins play an essential role in plant virus infection and host defense. The potyviral nuclear inclusion protein a protease (NIa-Pro) is involved in various steps of viral infection. In this study, the host proteins interacting with papaya ringspot virus (PRSV) NIa-Pro were screened in a Carica papaya L. plant cDNA library using a Sos recruitment two-hybrid system (SRS). We conﬁrmed that the full-length EIF3G, FBPA1, FK506BP, GTPBP, MSRB1, and MTL from papaya can interact speciﬁcally with PRSV NIa-Pro in yeast, respectively. These proteins fufill important functions in plant protein translation, biotic and abiotic stress, energy metabolism and signal transduction. In this paper, we discuss possible functions of interactions between these host proteins and NIa-Pro in PRSV infection and their role in host defense.
  
  
  BACKGROUND
  Sos recruitment two-hybrid system; papaya ringspot virus; NIa-Pro; protein-protein interaction.
authors.affiliation: College of Agriculture, Hainan University, Hainan, People's Republic of China.
authors.forename: L
authors.initials: L
authors.lastname: Gao
authors.forename: W T
authors.initials: WT
authors.lastname: Shen
authors.forename: P
authors.initials: P
authors.lastname: Yan
authors.forename: D C
authors.initials: DC
authors.lastname: Tuo
authors.forename: X Y
authors.initials: XY
authors.lastname: Li
authors.forename: P
authors.initials: P
authors.lastname: Zhou
mesh_terms: D029441:Carica / Q000276:immunology / Q000821:virology*; D003001:Cloning, Molecular; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D015723:Gene Library; D054884:Host-Pathogen Interactions; D010935:Plant Diseases / Q000276:immunology / Q000821:virology*; D010940:Plant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D010957:Plasmids; D017800:Potyvirus / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D015398:Signal Transduction / Q000276:immunology; D020798:Two-Hybrid System Techniques; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D014764:Viral Proteins; D003546:Cysteine Endopeptidases