title: A promising dehydrogenase-based bioanode for a glucose biosensor and glucose/O2 biofuel cell.
abstract: A new type of dehydrogenase-based amperometric glucose biosensor was constructed using glucose dehydrogenase (GDH) which was immobilized on the edge-plane pyrolytic graphite (EPPG) electrode modified with poly(phenosafranin)-functionalized single-walled carbon nanotubes (PPS-SWCNTs). The PPS-SWCNT-modified EPPG electrode was prepared by electropolymerization of phenosafranin on the EPPG electrode which had been previously coated with SWCNTs. The performance of the GDH/PPS-SWCNT/EPPG bioanode was evaluated using cyclic voltammetry and amperometry in the presence of glucose. The GDH/PPS-SWCNT/EPPG electrode possesses promising characteristics as a glucose sensor: a wide linear dynamic range of 50 to 700 μM, low detection limit of 0.3 μM, fast response time (1-2 s), high sensitivity (96.5 μA cm(-2) mM(-1)), and anti-interference and anti-fouling abilities. Moreover, the performance of the GDH/PPS-SWCNT/EPPG bioanode was tested in a glucose/O(2) biofuel cell. The maximum power density delivered by the assembled glucose/O(2) biofuel cell could reach 64.0 μW cm(-2) at a cell voltage of 0.3 V with 40 mM glucose.
authors.affiliation: Department of Electronic Chemistry, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8502, Japan.
authors.forename: Farhana S
authors.initials: FS
authors.lastname: Saleh
authors.forename: Lanqun
authors.initials: L
authors.lastname: Mao
authors.forename: Takeo
authors.initials: T
authors.lastname: Ohsaka
mesh_terms: D001407:Bacillus / Q000201:enzymology; D056804:Biofuels; D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005947:Glucose / Q000032:analysis* / Q000737:chemistry*; D042843:Glucose 1-Dehydrogenase / Q000737:chemistry* / Q000378:metabolism*; D006108:Graphite / Q000737:chemistry; D053761:Nanocomposites / Q000737:chemistry; D037742:Nanotubes, Carbon / Q000737:chemistry; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry*; D010619:Phenazines / Q000737:chemistry; D058105:Polymerization
chemical_list: D056804:Biofuels; D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D010619:Phenazines; D006108:Graphite; C011977:phenosafranine; D042843:Glucose 1-Dehydrogenase; D005947:Glucose; D010100:Oxygen