title: One-flask synthesis of cyclic diguanosine monophosphate (c-di-GMP).
abstract: The bacterial signaling molecule, cyclic diguanosine monophosphate (c-di-GMP), plays a key role in controlling biofilm formation and pathogenic virulence, among many other functions. It has widespread consequences for human health, and current research is actively exploring its molecular mechanisms. The convenient one-flask, gram-scale synthesis of c-di-GMP described here will facilitate these efforts.
authors.affiliation: Department of Chemistry and Chemical Biology, Rutgers University, Piscataway, New Jersey, USA.
authors.forename: Barbara L
authors.initials: BL
authors.lastname: Gaffney
authors.forename: Roger A
authors.initials: RA
authors.lastname: Jones
mesh_terms: D001419:Bacteria / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D003460:Crystallization / Q000295:instrumentation* / Q000379:methods*; D006152:Cyclic GMP / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D003500:Cyclization; D003999:Dichloroacetic Acid / Q000737:chemistry; D005021:Ethylamines / Q000737:chemistry; D011725:Pyridines / Q000737:chemistry; D012996:Solutions / Q000737:chemistry; D013997:Time Factors
chemical_list: D005021:Ethylamines; D011725:Pyridines; D012996:Solutions; C062025:bis(3',5')-cyclic diguanylic acid; D003999:Dichloroacetic Acid; D006152:Cyclic GMP; C023666:pyridine; C016162:triethylamine