title: Modulation of signal-transducing function of neuronal membrane Na+,K+-ATPase by endogenous ouabain and low-power infrared radiation leads to pain relief.
abstract: Effects of infrared (IR) radiation generated by a low-power Co2-laser on sensory neurons of chick embryos were investigated by organotypic culture method. Low-power IR radiation firstly results in marked neurite suppressing action, probably induced by activation of Na+,K+-ATPase signal-transducing function. A further increase in energy of radiation leads to stimulation of neurite growth. We suggest that this effect is triggered by activation of Na+,K+-ATPase pumping function. Involvement of Na+,K+-ATPase in the control of the transduction process was proved by results obtained after application of ouabain at very low concentrations. Physiological significance of low-power IR radiation and effects of ouabain at nanomolar level was investigated in behavioral experiments (formalin test). It is shown that inflammatory pain induced by injection of formalin is relieved both due to ouabain action and after IR irradiation.
authors.affiliation: Pavlov Institute of Physiology of Russian Academy of Sciences, St. Petersburg, Russia.
authors.forename: Ekaterina V
authors.initials: EV
authors.lastname: Lopatina
authors.forename: Igor L
authors.initials: IL
authors.lastname: Yachnev
authors.forename: Valentina A
authors.initials: VA
authors.lastname: Penniyaynen
authors.forename: Vera B
authors.initials: VB
authors.lastname: Plakhova
authors.forename: Svetlana A
authors.initials: SA
authors.lastname: Podzorova
authors.forename: Tatiana N
authors.initials: TN
authors.lastname: Shelykh
authors.forename: Ilya V
authors.initials: IV
authors.lastname: Rogachevsky
authors.forename: Irina P
authors.initials: IP
authors.lastname: Butkevich
authors.forename: Viktor A
authors.initials: VA
authors.lastname: Mikhailenko
authors.forename: Anna V
authors.initials: AV
authors.lastname: Kipenko
authors.forename: Boris V
authors.initials: BV
authors.lastname: Krylov
mesh_terms: D000818:Animals; D002642:Chick Embryo; D007259:Infrared Rays / Q000627:therapeutic use*; D028022:Low-Level Light Therapy; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D010042:Ouabain / Q000494:pharmacology* / Q000627:therapeutic use; D010146:Pain / Q000188:drug therapy* / Q000532:radiotherapy*; D059408:Pain Management; D010147:Pain Measurement; D018408:Patch-Clamp Techniques; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects / Q000528:radiation effects; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
chemical_list: D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase