title: Evaluation of cloned cells, animal model, and ATRA sensitivity of human testicular yolk sac tumor.
abstract: The testicular yolk sac tumor (TYST) is the most common neoplasm originated from germ cells differentiated abnormally, a major part of pediatric malignant testicular tumors. The present study aimed at developing and validating the in vitro and vivo models of TYST and evaluating the sensitivity of TYST to treatments, by cloning human TYST cells and investigating the histology, ultra-structure, growth kinetics and expression of specific proteins of cloned cells. We found biological characteristics of cloned TYST cells were similar to the yolk sac tumor and differentiated from the columnar to glandular-like or goblet cells-like cells. Chromosomes for tumor identification in each passage met nature of the primary tumor. TYST cells were more sensitive to all-trans-retinoic acid which had significantly inhibitory effects on cell proliferation. Cisplatin induced apoptosis of TYST cells through the activation of p53 expression and down-regulation of Bcl- expression. Thus, we believe that cloned TYST cells and the animal model developed here are useful to understand the molecular mechanism of TYST cells and develop potential therapies for human TYST.
authors.affiliation: Department of Pediatric Surgery, the Second Hospital, Wenzhou Medical College, Wenzhou, China.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Zhao
authors.forename: Congde
authors.initials: C
authors.lastname: Chen
authors.forename: Haochuan
authors.initials: H
authors.lastname: Zhang
authors.forename: Jinhui
authors.initials: J
authors.lastname: Shen
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.forename: Xiaokun
authors.initials: X
authors.lastname: Lin
authors.forename: Le
authors.initials: L
authors.lastname: Qin
authors.forename: Xiaozhou
authors.initials: X
authors.lastname: Bao
authors.forename: Jie
authors.initials: J
authors.lastname: Lin
authors.forename: Wenqiang
authors.initials: W
authors.lastname: Lu
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Wang
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Chen
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002675:Child, Preschool; D002945:Cisplatin / Q000494:pharmacology; D002999:Clone Cells; D018240:Endodermal Sinus Tumor / Q000188:drug therapy* / Q000473:pathology / Q000648:ultrastructure; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009363:Neoplasm Proteins / Q000378:metabolism; D013736:Testicular Neoplasms / Q000188:drug therapy* / Q000473:pathology / Q000648:ultrastructure; D014212:Tretinoin / Q000494:pharmacology / Q000627:therapeutic use*; D047368:Tumor Burden / Q000187:drug effects; D023041:Xenograft Model Antitumor Assays; D000509:alpha-Fetoproteins / Q000378:metabolism
chemical_list: D009363:Neoplasm Proteins; D000509:alpha-Fetoproteins; D014212:Tretinoin; D002945:Cisplatin