title: Polymeric proanthocyanidins in skins and seeds of 37 Vitis vinifera L. cultivars: a methodological comparative study.
abstract: The polymeric proanthocyanidins (PAs) composition of skins and seeds from different Vitis vinifera L. cv. was evaluated, sampling 37 cultivar, preserved in the grapevine collection of Grinzane Cavour (Piedmont, Italy). PAs were quantified using both spectrophotometric (n-butanol and vanillin assay) and chromatographic (high-performance liquid chromatography [HPLC]) methods, and the evaluation of their antioxidant activity was performed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay. Results of PAs quantification with the different methods were significantly correlated. A strong correlation pointed out between anthocyans and PAs content (particularly regarding HPLC method; r = 0.9359, P = 0), and the antioxidant activity was not dependent on the polymeric PAs content, but negatively correlated with the degree of polymerization (r =-0.6102, P = 1 × 10⁻⁴). The hierarchical clustering on principal components analysis allowed grouping the samples in 3 different clusters, observing a direct association between the cluster classification and the anthocyans/PAs content.
authors.affiliation: DiSCAFF and DFB-Drug and Food Biotechnology Center, Univ. Degli Studi del Piemonte Orientale "A. Avogadro", Via Bovio 6, Novara 28100, Italy.
authors.forename: Fabiano
authors.initials: F
authors.lastname: Travaglia
authors.forename: Matteo
authors.initials: M
authors.lastname: Bordiga
authors.forename: Monica
authors.initials: M
authors.lastname: Locatelli
authors.forename: Jean Daniel
authors.initials: JD
authors.lastname: Coïsson
authors.forename: Marco
authors.initials: M
authors.lastname: Arlorio
mesh_terms: D020001:1-Butanol / Q000032:analysis; D001547:Benzaldehydes / Q000032:analysis; D001713:Biphenyl Compounds / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005638:Fruit / Q000737:chemistry*; D015999:Multivariate Analysis; D010851:Picrates / Q000378:metabolism; D011108:Polymers / Q000032:analysis* / Q000737:chemistry; D044945:Proanthocyanidins / Q000032:analysis*; D012639:Seeds / Q000737:chemistry*; D013053:Spectrophotometry / Q000379:methods; D027843:Vitis / Q000737:chemistry*
chemical_list: D001547:Benzaldehydes; D001713:Biphenyl Compounds; D010851:Picrates; D011108:Polymers; D044945:Proanthocyanidins; D020001:1-Butanol; C100058:vanillin; C004931:1,1-diphenyl-2-picrylhydrazyl