title: A personal view from a long-lasting collaborator on the research strategies of Marshall Nirenberg.
abstract: In this review, I summarized transition in Dr. Marshall Nirenberg's research interests during 1970s, from a view of a long-lasting collaborator. Nirenberg switched his research filed to neurobiology after his success in deciphering genetic code and being honored with the Nobel Prize in Physiology or Medicine in 1968. His targets were to obtain genetically pure population of neurons, i.e. neuroblastoma clones, to make somatic hydrid cells, to culture neuronal and muscle cells, and to produce monoclonal antibodies against whole retinal or neuroblastoma cells. He studied neurotransmitters, receptors, cyclic nucleotides, cell differentiation, secretion, synapse formation, and chemical recognition. Especially he liked his hypothesis for opiate tolerance and dependency as a model of cellular memory. Through these studies, he seemed to devote all his time of about 50 years from 1960s to decoding brain memory processes.
authors.affiliation: Department of Biophysical Genetics, Kanazawa University Graduate School of Medicine, Kanazawa 920-8640, Japan. haruhiro@med.kanazawa-u.ac.jp
authors.forename: Haruhiro
authors.initials: H
authors.lastname: Higashida
mesh_terms: D000527:Alprostadil / Q000494:pharmacology; D000911:Antibodies, Monoclonal / Q000276:immunology; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D003299:Cooperative Behavior; D049673:History, 20th Century; D049674:History, 21st Century; D006801:Humans; D009447:Neuroblastoma / Q000473:pathology; D010716:Phosphatidylinositols / Q000378:metabolism; D018002:Receptors, Bradykinin / Q000378:metabolism; D012106:Research; D015398:Signal Transduction; D013806:Theophylline / Q000494:pharmacology
chemical_list: D000911:Antibodies, Monoclonal; D010716:Phosphatidylinositols; D018002:Receptors, Bradykinin; D013806:Theophylline; D000527:Alprostadil