title: Cloning, overexpression in Escherichia coli, and characterization of a thermostable fungal acetylxylan esterase from Talaromyces emersonii.
abstract: The gene encoding an acetylxylan esterase (AXE1) from the thermophilic ascomycete Talaromyces emersonii was cloned, expressed in Escherichia coli, and characterized. This form of AXE1, rTeAXE1, exhibits increased thermostability and activity at a higher temperature than other known fungal acetyl esterases, thus having huge potential application in biomass bioconversion to high value chemicals or biofuels.
authors.affiliation: Molecular Glycobiotechnology Group, Biochemistry Department, School of Natural Sciences, National University of Ireland Galway, Galway, Ireland. d.waters@ucc.ie
authors.forename: Deborah M
authors.initials: DM
authors.lastname: Waters
authors.forename: Patrick G
authors.initials: PG
authors.lastname: Murray
authors.forename: Yuta
authors.initials: Y
authors.lastname: Miki
authors.forename: Angel T
authors.initials: AT
authors.lastname: Martínez
authors.forename: Maria G
authors.initials: MG
authors.lastname: Tuohy
authors.forename: Craig B
authors.initials: CB
authors.lastname: Faulds
mesh_terms: D000115:Acetylesterase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004795:Enzyme Stability; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D032901:Talaromyces / Q000201:enzymology* / Q000235:genetics*; D013696:Temperature
chemical_list: D000115:Acetylesterase; C081851:acetylxylan esterase