title: Induction of cell migration in vitro by an electrospun PDGF-BB/PLGA/PEG-PLA nanofibrous scaffold.
abstract: Electrospinning is a versatile technique used to fabricate potential tissue engineering scaffolds with a structure similar to the native extracellular matrix (ECM). In this study, Platelet-Derived Growth Factor (PDGF)-BB with BSA as a carrier protein was incorporated into an electrospun PLGA/PEG-PLA composite scaffold for induction of cell migration, an early process necessary for tissue regeneration and wound healing. Incorporating PDGF-BB into the fibers did not change the overall morphology of the scaffold, with the exception of a slight increase (approximately 12%) in the number of fibers with diameters ranging from 1-100 nm. Following a strong burst of release during the initial 24 hours, approximately 20% of the total incorporated PDGF-BB was released from the scaffold over 5 days, as determined by ELISA. The presence of the released PDGF-BB was also confirmed via SDS-PAGE. Using an in vitro agarose-cell migration assay with MC3T3 pre-osteoblastic cells, the preserved bioactivity of the released PDGF-BB was demonstrated via its ability to stimulate robust cell migration, equivalent to that of pure unincorporated (control) PDGF-BB. Overall, this study demonstrates that it is feasible to incorporate and deliver bioactive PDGF-BB via an electrospun scaffold for potential tissue repair applications, especially as a potent inducer of cell migration.
authors.affiliation: Department of Biomedical Engineering, Stony Brook University, Stony Brook, NY 11794-5281, USA.
authors.forename: Xia
authors.initials: X
authors.lastname: Zhao
authors.forename: Michael
authors.initials: M
authors.lastname: Hadjiargyrou
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000077214:Becaplermin; D018929:Cell Culture Techniques / Q000379:methods; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects*; D055664:Electrochemical Techniques; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D057139:Nanofibers / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology*; D011100:Polyglycolic Acid / Q000737:chemistry / Q000494:pharmacology*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D020574:Proto-Oncogene Proteins c-sis / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D020574:Proto-Oncogene Proteins c-sis; C542623:monomethoxypolyethyleneglycol-polylactide block copolymer; D000077214:Becaplermin; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D011092:Polyethylene Glycols