title: Biopolymer gel matrix as acellular scaffold for enhanced dermal tissue regeneration.
abstract: Biological grafts have drawbacks such as donor scarcity, disease transmission, tissue infection, while the scaffolds of either collagen or chitosan fabrics fail to become part of the tissue at the wound site, though they favor the formation of connective tissue matrix. This study developed a novel composite consisting of the combination of atelocollagen and chitosan in order to provide a biodegradable molecular matrix in gel form as a biomimetic surface for cell attachment, to promote the wound healing in excision wounds. We found that the topical application of biopolymer composite on the wound promoted cell proliferation, migration and collagen deposition overtime. The enhanced cellular activity in the collagen-chitosan treated wound tissue was also assed by increased levels of Platelet derived growth factor (PDGF) and Nerve growth factor (NGF) associated with elevated levels of antioxidants and decreased level of lipid peroxidation. The acellular matrix-like topical application material is designed to guide the eventual re-establishment of an anatomically normal skin. The results of this study demonstrate the feasibility of multi-cell regeneration on a molecular system that mimics tissue engineering in vivo.
authors.affiliation: Department of Biotechnology, CSIR-Central Leather Research Institute, Adyar, Chennai 600020, Tamil Nadu, India.
authors.forename: Rangasamy
authors.initials: R
authors.lastname: Judith
authors.forename: Mariappan
authors.initials: M
authors.lastname: Nithya
authors.forename: Chellan
authors.initials: C
authors.lastname: Rose
authors.forename: Asit Baran
authors.initials: AB
authors.lastname: Mandal
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001672:Biocompatible Materials; D001704:Biopolymers; D048271:Chitosan; D003094:Collagen; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005782:Gels; D015227:Lipid Peroxidation; D020932:Nerve Growth Factor / Q000378:metabolism; D010982:Platelet-Derived Growth Factor / Q000378:metabolism; D051381:Rats; D012038:Regeneration; D012867:Skin / Q000378:metabolism; D012879:Skin Physiological Phenomena
chemical_list: D000975:Antioxidants; D001672:Biocompatible Materials; D001704:Biopolymers; D005782:Gels; D010982:Platelet-Derived Growth Factor; D003094:Collagen; D048271:Chitosan; D020932:Nerve Growth Factor