title: Associated expression of α2,3sialylated type 2 chain structures with lymph node metastasis in distal colorectal cancer.
abstract: OBJECTIVE
  One of the typical modifications on the surface of cancer cells is sialylation of terminal carbohydrates. The expression of several types of sialylation of glycoconjugates was investigated in colorectal cancer.
  
  
  METHODS
  The cancer tissue specimens obtained from 65 colorectal cancer patients were stained with sialic acid-binding lectins from Maackia amurensis (MAM), Sambucus sieboldiana (SSA), Maackia amurensis agglutinin (MAA) and monoclonal antibodies, and compared with their clinicopathological features.
  
  
  RESULTS
  Cancer tissue specimens from 44.6% of patients had positive staining with MAM, which recognized α2,3sialylated type 2 chain (NeuAcα2,3Galβ1,4GlcNAcβR) structures, but normal colorectal mucosa showed only weak staining with MAM was observed. More lymph node metastases and lymphatic invasion were seen in patients with positive staining with MAM (P < 0.01), while not with other lectins or antibodies that recognized sialylated glycoconjugates or sialyl Lewis-related antigens. The five-year survival rate of patients with MAM-positive staining was significantly lower than that with MAM-negative staining when including T0-1 cases, but there was no difference in cases with T2-4. There was no difference in the patients' survival rates when the tissues were stained with MAA, SSA or PNA lectins.
  
  
  CONCLUSIONS
  α2,3Sialylated type 2 chain structures were predominantly expressed in colorectal tissues associated with the malignant transformation, in particular, with lymphatic spread of distal colorectal adenocarcinomas.
authors.affiliation: Department of General Surgical Science, Gunma University Graduate School of Medicine, Showa-machi, Maebashi, Gunma 371-8511, Japan.
authors.forename: Takaharu
authors.initials: T
authors.lastname: Fukasawa
authors.forename: Takayuki
authors.initials: T
authors.lastname: Asao
authors.forename: Hayato
authors.initials: H
authors.lastname: Yamauchi
authors.forename: Munenori
authors.initials: M
authors.lastname: Ide
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Tabe
authors.forename: Takaaki
authors.initials: T
authors.lastname: Fujii
authors.forename: Satoru
authors.initials: S
authors.lastname: Yamaguchi
authors.forename: Soichi
authors.initials: S
authors.lastname: Tsutsumi
authors.forename: Shin
authors.initials: S
authors.lastname: Yazawa
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kuwano
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000401:mortality / Q000473:pathology* / Q000601:surgery; D014408:Biomarkers, Tumor / Q000737:chemistry / Q000378:metabolism*; D002471:Cell Transformation, Neoplastic / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D003082:Colectomy; D015179:Colorectal Neoplasms / Q000378:metabolism / Q000401:mortality / Q000473:pathology* / Q000601:surgery; D005260:Female; D006001:Glycoconjugates / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008207:Lymphatic Metastasis; D008297:Male; D037121:Plant Lectins; D012007:Rectum / Q000601:surgery; D012794:Sialic Acids / Q000737:chemistry / Q000378:metabolism*; D015996:Survival Rate; D016896:Treatment Outcome
chemical_list: D014408:Biomarkers, Tumor; D006001:Glycoconjugates; D037121:Plant Lectins; D012794:Sialic Acids