title: Monolithic column based on a poly(glycidyl methacrylate-co-4-vinylphenylboronic acid-co-ethylene dimethacrylate) copolymer for capillary liquid chromatography of small molecules and proteins.
abstract: A new polymer monolith with three modes of reverse-phase, hydrophilic and cation-exchange interaction was synthesized in 100 μm i.d. fused-silica capillary by in situ polymerization procedure. The pre-polymerization mixture consisted of glycidyl methacrylate (GMA) and 4-vinylphenylboronic acid (VPBA) as bifunctional monomers, ethylene dimethacrylate (EDMA) as crosslinker, 1,4-butanediol (BDO) and diethylene glycol (DEG) as binary porogenic solvents, and azobisisobutyronitrile (AIBN) as initiator. The resulting poly(GMA-co-VPBA-co-EDMA) monolith showed a relatively homogeneous monolithic structure, good permeability and mechanical stability. Different ratios of monomers and porogens were used for optimizing the properties of monolithic column. The column performance was assessed by the separation of a series of neutral solutes, charge solutes, phenols and anilines. Compared with poly(GMA-co-EDMA) monolith, the proposed monolith exhibited more flexible adjustment of selectivity in terms of hydrophobic, hydrophilic, as well as cation-exchange interaction in the same chromatographic conditions. High column efficiencies for benzene derivatives with 70,000-102,000 theoretical plates/m could be obtained at a linear velocity of 0.265 mm/s. The run-to-run, column-to-column, and batch-to-batch repeatabilities of the retention times were less than 8.23%. Additionally, the purposed monolith was also applied to efficient separation of alkaloids and proteins for demonstrating its potential in biomolecule separation.
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China. zianlin@fzu.edu.cn
authors.forename: Zian
authors.initials: Z
authors.lastname: Lin
authors.forename: Hui
authors.initials: H
authors.lastname: Huang
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Sun
authors.forename: Yao
authors.initials: Y
authors.lastname: Lin
authors.forename: Lan
authors.initials: L
authors.lastname: Zhang
authors.forename: Guonan
authors.initials: G
authors.lastname: Chen
mesh_terms: D000180:Acrylic Resins / Q000138:chemical synthesis / Q000737:chemistry*; D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification; D001555:Benzene Derivatives / Q000737:chemistry / Q000302:isolation & purification; D001897:Boronic Acids / Q000138:chemical synthesis / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000295:instrumentation* / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D008689:Methacrylates / Q000737:chemistry*; D009994:Osmolar Concentration; D011109:Polymethacrylic Acids / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification; D015203:Reproducibility of Results; D014753:Vinyl Compounds / Q000737:chemistry*
chemical_list: C427523:4-vinylphenyl boronic acid; D000180:Acrylic Resins; D000470:Alkaloids; D001555:Benzene Derivatives; D001897:Boronic Acids; D008689:Methacrylates; D011109:Polymethacrylic Acids; D011506:Proteins; D014753:Vinyl Compounds; C042535:polyglycidyl methacrylate; C004919:ethylene dimethacrylate