title: Susceptibility assay of Haemonchus contortus to commonly used anthelmintics in Jimma, southwest Ethiopia.
abstract: An experimental study to investigate the susceptibility pattern of Haemonchus contortus to commonly used anthelmintics (albendazole, tetramisole, ivermectin, and triclobendazole) was conducted between June and September 2009. Adult H. contortus parasites were collected from a total of 30 sheep slaughtered at Jimma municipal abattoir. The anthelmintics were assessed for egg hatch inhibition ability against H. contortus eggs. The eggs (500 eggs/ml) were incubated with different concentrations (0.25, 0.125, 0.0625, 0.03125, and 0.015625 μg/ml) at 26°C for 48 h. Egg hatching inhibition of different anthelmintics at different concentrations was recorded. The overall mean percent inhibition showed that there was a significant difference (p < 0.05) among the different concentrations. Mean egg hatch inhibition of ivermectin showed the highest result (84.90 %) at 0.25 μg/ml, followed by albendazole (78.77 %), triclobendazole (76.66 %), and tetramisole (78.98 %), respectively. The overall mean percent inhibition showed a significant difference (p < 0.05) among the different anthelmintics. Further evaluation of these anthelmintics was recommended.
authors.affiliation: College of Agriculture and Veterinary Medicine, Jimma University, P.O. Box 307, Jimma, Ethiopia.
authors.forename: Sileshi
authors.initials: S
authors.lastname: Belew
authors.forename: Jemal
authors.initials: J
authors.lastname: Hussien
authors.forename: Feyesa
authors.initials: F
authors.lastname: Regassa
authors.forename: Kumilachew
authors.initials: K
authors.lastname: Belay
authors.forename: Tadele
authors.initials: T
authors.lastname: Tolosa
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D001562:Benzimidazoles / Q000494:pharmacology*; D005002:Ethiopia; D005260:Female; D006189:Haemonchus / Q000187:drug effects*; D007559:Ivermectin / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects; D007928:Lethal Dose 50; D008297:Male; D010063:Ovum / Q000187:drug effects; D021261:Parasitic Sensitivity Tests / Q000662:veterinary; D013773:Tetramisole / Q000494:pharmacology*
chemical_list: D000871:Anthelmintics; D001562:Benzimidazoles; D007559:Ivermectin; D013773:Tetramisole