title: Soy protein films for wound-healing applications: antibiotic release, bacterial inhibition and cellular response.
abstract: Use of naturally derived materials is becoming widespread in the biomedical field. Soy protein has advantages over the various types of natural proteins employed for biomedical applications, due to its low price, non-animal origin and relatively long storage time and stability. In the current study, soy protein isolate (SPI) was investigated as a matrix for wound-dressing applications. The antibiotic drug gentamicin was incorporated into the matrix for local controlled release and thus continuous bactericidal effect. Homogeneous high-quality films were cast from aqueous solutions and tested for the effects of gentamicin release on bacterial inhibition. The cytotoxicity and in vitro biocompatibility of these films were also examined. The gentamicin release profiles exhibited a moderate burst effect followed by a decreasing release rate, which was maintained for at least 4 weeks, thus enabling a suitable bacterial inhibition effect. The materials released from the films during an indirect cytotoxicity test were found to be safe, except for a slight inhibitory effect in the presence of high concentrations of glycerol. The biocompatibility test showed confluent cell cultures in close proximity to the SPI films. It is clear that these new antibiotic-eluting SPI films exhibit a high potential for use as wound dressings.
authors.affiliation: Department of Biomedical Engineering, Tel-Aviv University, Israel.
authors.forename: Zachi
authors.initials: Z
authors.lastname: Peles
authors.forename: Itzhak
authors.initials: I
authors.lastname: Binderman
authors.forename: Israela
authors.initials: I
authors.lastname: Berdicevsky
authors.forename: Meital
authors.initials: M
authors.lastname: Zilberman
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D002452:Cell Count; D002478:Cells, Cultured; D004058:Diffusion; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects; D005839:Gentamicins / Q000494:pharmacology; D006801:Humans; D008297:Male; D008422:Materials Testing; D008826:Microbial Sensitivity Tests; D030262:Soybean Proteins / Q000494:pharmacology*; D013314:Stress, Mechanical; D013718:Tensile Strength / Q000187:drug effects; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D005839:Gentamicins; D030262:Soybean Proteins