title: Use of intestinal Pseudomonas aeruginosa in fish to detect the environmental pollutant benzo[a]pyrene.
abstract: This study examined the potential of Pseudomonas aeruginosa abundance in the intestines of fish as an indicator of exposure to benzo[a]pyrene (BaP). P. aeruginosa populations were enumerated in juvenile African catfish (Clarias gariepinus) injected intramuscularly three days previous with 0, 10, 30, 40, 50 or 70mg/kg of BaP. Hepatic EROD and GST activities and biliary fluorescent aromatic compounds (FACs) 1-OH BaP, 3-OH BaP, 7,8-D BaP and BaP were quantified to investigate agreements between the new indicator and established fish biomarkers. The shape of bacterial population (logarithm of colony-forming unit) dose-response curve generally matched those of biliary FACs concentrations. Conversely, the EROD and GST dose-response curves were generally the mirror images of the bacterial population curve. Changes in intestinal P. aeruginosa population appear to be an indirect effect of BaP exposure because exposure to 0-100μg/ml BaP had no effect on P. aeruginosa populations grown on agar plates containing BaP. Using intestinal P. aeruginosa population of fish as a universal indicator of BaP pollution in aquatic environments is discussed.Conversely, the EROD and GST dose-response curves were generally the mirror images of the bacterial population curve.
authors.affiliation: Department of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Selangor, Malaysia. alikaramiv@gmail.com
authors.forename: Ali
authors.initials: A
authors.lastname: Karami
authors.forename: Annie
authors.initials: A
authors.lastname: Christianus
authors.forename: Zamri
authors.initials: Z
authors.lastname: Ishak
authors.forename: Zulkifli Hj
authors.initials: ZH
authors.lastname: Shamsuddin
authors.forename: Majid
authors.initials: M
authors.lastname: Masoumian
authors.forename: Simon C
authors.initials: SC
authors.lastname: Courtenay
mesh_terms: D000818:Animals; D001564:Benzo(a)pyrene / Q000032:analysis* / Q000633:toxicity; D002397:Catfishes / Q000378:metabolism / Q000382:microbiology; D015169:Colony Count, Microbial; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D004784:Environmental Monitoring / Q000379:methods; D005982:Glutathione Transferase / Q000378:metabolism; D007422:Intestines / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification*; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000633:toxicity
chemical_list: D014874:Water Pollutants, Chemical; D001564:Benzo(a)pyrene; D019363:Cytochrome P-450 CYP1A1; D005982:Glutathione Transferase