title: Expression and purification of recombinant type IV fimbrial subunit protein of Pasteurella multocida serogroup B:2 in Escherichia coli.
abstract: Pasteurella multocida serogroup B:2, a causative agent of haemorrhagic secpticaemia (HS) in cattle and buffalo especially in tropical regions of Asia and African countries, is known to possess a type IV fimbriae (pili) as one of the virulent factors. In the present study, ptfA gene encoding for type IV fimbrial subunit of P. multocida serogroup B:2 (strain p52), an Indian HS vaccine strain, has been cloned and over-expressed in recombinant Escherichia coli. The recombinant type IV fimbrial subunit protein (∼31 kDa) including N-terminus histidine tag was purified under denaturing condition and confirmed by western blotting. A homology model of HS causing P. multocida serogroup B:2 fimbrial subunit has also been discussed. The study indicated the potential possibilities to use the recombinant fimbrial protein in developing HS subunit vaccine along with suitable adjuvant.
authors.affiliation: Clinical Bacteriology Laboratory (CBL), Indian Veterinary Research Institute (IVRI), Regional Campus, Mukteswar-263138, Nainital (District), Uttarakhand (UK), India. sbshivachandra@gmail.com
authors.forename: Sathish B
authors.initials: SB
authors.lastname: Shivachandra
authors.forename: Revanaiah
authors.initials: R
authors.lastname: Yogisharadhya
authors.forename: Anuj
authors.initials: A
authors.lastname: Ahuja
authors.forename: Veerakyathappa
authors.initials: V
authors.lastname: Bhanuprakash
mesh_terms: D000595:Amino Acid Sequence; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D036781:Fimbriae Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D008958:Models, Molecular; D058977:Molecular Sequence Annotation; D016979:Pasteurella multocida / Q000378:metabolism*; D011487:Protein Conformation; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism
chemical_list: D021122:Protein Subunits; D036781:Fimbriae Proteins