title: Modulating vascular intimal hyperplasia using HSV-1 mutant requires activated MEK.
abstract: Outcomes of cardiovascular procedures, such as angioplasty and stent or bypass grafting are limited by failure, predominantly caused by pathological smooth muscle cell (SMC) proliferation, known as intimal hyperplasia. Local delivery of a genetically engineered herpes simplex virus (HSV) is known to block vascular SMC proliferation while allowing for re-endothelialization. However, the mechanism this mutant virus uses to prevent SMC hyperplasia is unknown. The Ras signaling cascade is activated in SMCs undergoing hyperplasia leading to phosphorylation of the mitogen-activated protein kinase (MAPK). In this study we tested the hypothesis that MAPK kinase (MEK) activity is the molecular basis by which SMCs are susceptible to mutant HSV. We show that genetically engineered herpes simplex-1 viruses (HSV-1) can target proliferating SMCs. We demonstrate that the molecular basis of this HSV-1 anti-proliferative effect is MEK activation in SMCs. We demonstrate efficacy and practicality of the MEK-dependent HSV-1 for the treatment of intimal hyperplasia in a clinically relevant in vivo model. Important to this strategy is the ability to modulate the effects by controlling viral dose. These results propel genetically engineered HSV-1 therapy towards clinical evaluation in treatment of intimal hyperplasia.
authors.affiliation: Section of Vascular Surgery, Department of Surgery, University of Chicago Medical Center, Chicago, IL 60637, USA. cskelly@surgery.bsd.uchicago.edu
authors.forename: C L
authors.initials: CL
authors.lastname: Skelly
authors.forename: Q
authors.initials: Q
authors.lastname: He
authors.forename: L
authors.initials: L
authors.lastname: Spiguel
authors.forename: S
authors.initials: S
authors.lastname: McCormick
authors.forename: R
authors.initials: R
authors.lastname: Weichselbaum
mesh_terms: D000818:Animals; D002339:Carotid Arteries / Q000473:pathology*; D002460:Cell Line; D049109:Cell Proliferation; D006801:Humans; D006965:Hyperplasia / Q000517:prevention & control / Q000628:therapy; D048369:MAP Kinase Kinase 1 / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000378:metabolism* / Q000473:pathology; D009154:Mutation; D032389:Myocytes, Smooth Muscle / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D011817:Rabbits; D015398:Signal Transduction; D018139:Simplexvirus / Q000235:genetics* / Q000378:metabolism; D017539:Tunica Intima / Q000473:pathology*; D018631:ras Proteins / Q000378:metabolism
chemical_list: D048369:MAP Kinase Kinase 1; D018631:ras Proteins