title: In vitro neutralisation of rotavirus infection by two broadly specific recombinant monovalent llama-derived antibody fragments.
abstract: Rotavirus is the main cause of viral gastroenteritis in young children. Therefore, the development of inexpensive antiviral products for the prevention and/or treatment of rotavirus disease remains a priority. Previously we have shown that a recombinant monovalent antibody fragment (referred to as Anti-Rotavirus Proteins or ARP1) derived from a heavy chain antibody of a llama immunised with rotavirus was able to neutralise rotavirus infection in a mouse model system. In the present work we investigated the specificity and neutralising activity of two llama antibody fragments, ARP1 and ARP3, against 13 cell culture adapted rotavirus strains of diverse genotypes. In addition, immunocapture electron microscopy (IEM) was performed to determine binding of ARP1 to clinical isolates and cell culture adapted strains. ARP1 and ARP3 were able to neutralise a broad variety of rotavirus serotypes/genotypes in vitro, and in addition, IEM showed specific binding to a variety of cell adapted strains as well as strains from clinical specimens. These results indicated that these molecules could potentially be used as immunoprophylactic and/or immunotherapeutic products for the prevention and/or treatment of infection of a broad range of clinically relevant rotavirus strains.
authors.affiliation: Enteric Virus Unit, Centre for Infections, Health Protection Agency, London, United Kingdom.
authors.forename: Farah
authors.initials: F
authors.lastname: Aladin
authors.forename: Alexandra W C
authors.initials: AW
authors.lastname: Einerhand
authors.forename: Janneke
authors.initials: J
authors.lastname: Bouma
authors.forename: Sandra
authors.initials: S
authors.lastname: Bezemer
authors.forename: Pim
authors.initials: P
authors.lastname: Hermans
authors.forename: Danielle
authors.initials: D
authors.lastname: Wolvers
authors.forename: Kate
authors.initials: K
authors.lastname: Bellamy
authors.forename: Leon G J
authors.initials: LG
authors.lastname: Frenken
authors.forename: Jim
authors.initials: J
authors.lastname: Gray
authors.forename: Miren
authors.initials: M
authors.lastname: Iturriza-Gómara
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology* / Q000627:therapeutic use; D000918:Antibody Specificity; D015153:Blotting, Western; D002161:Camelids, New World / Q000276:immunology*; D003968:Diarrhea, Infantile / Q000821:virology; D005838:Genotype; D006801:Humans; D007114:Immunization; D007128:Immunoglobulin Fragments / Q000276:immunology* / Q000627:therapeutic use; D007223:Infant; D051379:Mice; D011994:Recombinant Proteins / Q000276:immunology* / Q000627:therapeutic use; D012401:Rotavirus / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D012400:Rotavirus Infections / Q000188:drug therapy / Q000821:virology*
chemical_list: D057134:Antibodies, Neutralizing; D007128:Immunoglobulin Fragments; D011994:Recombinant Proteins