title: Evaluating EDTA as a substitute for phosphoric acid-etching of enamel and dentin.
abstract: Matrix metalloproteinases (MMPs) are proteolytic enzymes released when dentin is acid-etched. The enzymes are capable of destroying unprotected collagen fibrils that are not encapsulated by the dentin adhesive. Chlorhexidine applied after etching inhibits the activation of released MMPs, whereas neutral ethylenediamine tetra-acetic acid (EDTA) prevents the release of MMPs. The purpose of this study was to determine if conditioning enamel and dentin with EDTA can be a substitute for treating acid-etching enamel and dentin with chlorhexidine. A column of composite resin was bonded to enamel and dentin after conditioning. Shear bond strengths were evaluated after 48 hours and after accelerated aging for three hours in 12% sodium hypochlorite.  Shear bond strengths ranged from 15.6 MP a for accelerated aged EDTA enamel specimens to 26.8 MPa for dentin conditioned with EDTA and tested after 48 hours. A three-way ANOVA and a Tukey HSD test found statistically significant differences among the eight groups and the three independent variables (P < 0.05). EDTA was successfully substituted for phosphoric acid-etched enamel and dentin treated with chlorhexidine. Interactions of conditioning agent and aging were significant for dentin but not for enamel. In an effort to reduce the detrimental effects of MMPs, conditioning enamel and dentin with EDTA is an alternative to treating acid-etched dentin and enamel with chlorhexidine.
authors.affiliation: Department of General Practice, Virginia Commonwealth University School of Dentistry in Richmond, USA.
authors.forename: Terence A
authors.initials: TA
authors.lastname: Imbery
authors.forename: Matthew
authors.initials: M
authors.lastname: Kennedy
authors.forename: Charles
authors.initials: C
authors.lastname: Janus
authors.forename: Peter C
authors.initials: PC
authors.lastname: Moon
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods*; D002614:Chelating Agents / Q000737:chemistry* / Q000494:pharmacology; D002710:Chlorhexidine / Q000737:chemistry / Q000494:pharmacology; D003188:Composite Resins / Q000737:chemistry; D001840:Dental Bonding; D003743:Dental Enamel / Q000648:ultrastructure*; D003764:Dental Materials / Q000737:chemistry; D003799:Dental Stress Analysis / Q000295:instrumentation; D003804:Dentin / Q000648:ultrastructure*; D004492:Edetic Acid / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D006813:Humidity; D008422:Materials Testing; D061965:Matrix Metalloproteinase Inhibitors / Q000494:pharmacology; D010756:Phosphoric Acids / Q000737:chemistry*; D019279:Resin Cements / Q000737:chemistry; D033081:Shear Strength; D012973:Sodium Hypochlorite / Q000737:chemistry; D013314:Stress, Mechanical; D013696:Temperature; D013997:Time Factors
chemical_list: D002614:Chelating Agents; D003188:Composite Resins; C113175:Consepsis; D003764:Dental Materials; C444701:Filtek Z250; D061965:Matrix Metalloproteinase Inhibitors; C451600:OptiBond Solo Plus; D010756:Phosphoric Acids; D019279:Resin Cements; D004492:Edetic Acid; D012973:Sodium Hypochlorite; C030242:phosphoric acid; D002710:Chlorhexidine