title: Impact of immobilization support on colorimetric microarrays performances.
abstract: We report here a comparison of support materials for colorimetric hybridization assays on microarrays. Four surfaces with various chemistries and architectures (roughness and porosity) were evaluated: (i) bare and (ii) activated polystyrene surfaces classically used for ELISA; (iii) a double-sided adhesive support; and (iv) a porous nitrocellulose/cellulose acetate membrane. Each substrate was functionalized with a microarray of probes and subjected to an enzymatic colorimetric DNA hybridization test. Tests were carried out in a 96-well assembly suitable for automated high-throughput analysis. Colorimetry results, microscopy observations and a chemiluminescence study showed that the test efficiency not only depends on the surface probe density but that the capacity of the material to retain the colored enzymatic product is also a critical parameter. All parameters being considered, the adhesive coated surface proposes the best surface properties for efficient colorimetric microarrays.
authors.affiliation: Laboratoire de Génie Enzymatique, Membranes Biomimétiques et Assemblages Supramoléculaires, Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, Université Claude Bernard Lyon 1 - University of Lyon - CNRS 5246 ICBMS, Bât. CPE - 43 Bd du 11 Nov. 1918, 69622 Villeurbanne, France.
authors.forename: Gaelle C
authors.initials: GC
authors.lastname: Le Goff
authors.affiliation: Laboratoire de Génie Enzymatique, Membranes Biomimétiques et Assemblages Supramoléculaires, Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, Université Claude Bernard Lyon 1 - University of Lyon - CNRS 5246 ICBMS, Bât. CPE - 43 Bd du 11 Nov. 1918, 69622 Villeurbanne, France; AXO Science SAS, 34 rue du mail, 69004 Lyon, France.
authors.forename: Benjamin P
authors.initials: BP
authors.lastname: Corgier
authors.affiliation: Laboratoire de Génie Enzymatique, Membranes Biomimétiques et Assemblages Supramoléculaires, Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, Université Claude Bernard Lyon 1 - University of Lyon - CNRS 5246 ICBMS, Bât. CPE - 43 Bd du 11 Nov. 1918, 69622 Villeurbanne, France.
authors.forename: Céline A
authors.initials: CA
authors.lastname: Mandon
authors.affiliation: UMR CNRS 5280, Université Claude Bernard Lyon 1, Bât. CPE 43, bd du 11 Nov. 1918 - 69622 Villeurbanne, Cedex, France.
authors.forename: Gabriel
authors.initials: G
authors.lastname: De Crozals
authors.affiliation: UMR CNRS 5280, Université Claude Bernard Lyon 1, Bât. CPE 43, bd du 11 Nov. 1918 - 69622 Villeurbanne, Cedex, France.
authors.forename: Carole
authors.initials: C
authors.lastname: Chaix
authors.affiliation: Laboratoire de Génie Enzymatique, Membranes Biomimétiques et Assemblages Supramoléculaires, Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, Université Claude Bernard Lyon 1 - University of Lyon - CNRS 5246 ICBMS, Bât. CPE - 43 Bd du 11 Nov. 1918, 69622 Villeurbanne, France.
authors.forename: Loïc J
authors.initials: LJ
authors.lastname: Blum
authors.affiliation: Laboratoire de Génie Enzymatique, Membranes Biomimétiques et Assemblages Supramoléculaires, Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, Université Claude Bernard Lyon 1 - University of Lyon - CNRS 5246 ICBMS, Bât. CPE - 43 Bd du 11 Nov. 1918, 69622 Villeurbanne, France. Electronic address: christophe.marquette@univ-lyon1.fr.
authors.forename: Christophe A
authors.initials: CA
authors.lastname: Marquette
mesh_terms: D001483:Base Sequence; D003124:Colorimetry / Q000295:instrumentation*; D015342:DNA Probes / Q000235:genetics; D004867:Equipment Design; D057166:High-Throughput Screening Assays / Q000295:instrumentation; D006801:Humans; D046228:Microarray Analysis / Q000295:instrumentation*; D008855:Microscopy, Electron, Scanning; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation; D020641:Polymorphism, Single Nucleotide; D013499:Surface Properties
chemical_list: D015342:DNA Probes