title: The cytochrome c gene proximal enhancer drives activity-dependent reporter gene expression in hippocampal neurons.
abstract: The proximal enhancer of the cytochrome c gene (Cycs) contains binding sites for both cAMP response element binding proteins (CREB) and Nuclear Respiratory Factor 1 (NRF1). To investigate how neuronal activity regulates this enhancer region, a lentivirus was constructed in which a short-lived green fluorescent protein (GFP) was placed under the transcriptional control of the Cycs proximal enhancer linked to a synthetic core promoter. Primary hippocampal neurons were infected, and the synaptic strengths of individual neurons were measured by whole-cell patch clamping. On average the amplitude of miniature postsynaptic currents (mEPSCs) was higher in brighter GFP(+) neurons, while the frequency of mEPSCs was not significantly different. Inhibiting neural activity by applying a GABAA receptor agonist increased GFP expression in most neurons, which persisted after homeostatic synaptic scaling as evidenced by a decrease in the amplitude and frequency of mEPSCs. Removing the CREB binding sites revealed that calcium influx through L-type channels and NMDA receptors, and ERK1/2 activation played a role in NRF1-mediated transcription. CREB and NRF1, therefore, combine to regulate transcription of Cycs in response to changing neural activity.
authors.affiliation: The Neurosciences Institute, 10640 John Jay Hopkins Drive, San Diego CA, USA.
authors.forename: Jary Y
authors.initials: JY
authors.lastname: Delgado
authors.forename: Geoffrey C
authors.initials: GC
authors.lastname: Owens