title: Carboxymethylated dextran-coated magnetic iron oxide nanoparticles for regenerable bioseparation.
abstract: Carboxymethylated dextran (CMD)-coated magnetic iron oxide nanoparticles (MNPs) were synthesized using a co-precipitation method. Compared to neutral dextran coated MNPs, the CMD coating provides good dispersity and colloidal stability to the CMD-MNPs. In particular, the carboxyl groups on the CMD can be readily activated for covalent attachment of antibody molecules. The superparamagnetic property of the antibody-covered CMD-MNPs enables the captured antigen to be separated from the sample solution and CMD coating significantly reduces the nonspecific binding of the nanoparticles. Regeneration of the anti-BSA antibody-covered CMD-MNPs with NaOH does not significantly decrease the antibody activity, and the repeated magnetic separation and washing steps cause only small loss of the starting materials. The method was found to be highly reproducible (RSDs for BSA adsorption and desorption are between 0.78% and 5.1%). The anti-BSA antibody-covered CMD-MNPs possess good selectivity and are able to capture protein antigens from real samples.
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan 410083, P. R. China.
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhou
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.forename: Ning
authors.initials: N
authors.lastname: Xia
authors.forename: Qiyu
authors.initials: Q
authors.lastname: Huang
authors.forename: Huy
authors.initials: H
authors.lastname: Do
authors.forename: You-Nian
authors.initials: YN
authors.lastname: Liu
authors.forename: Feimeng
authors.initials: F
authors.lastname: Zhou
mesh_terms: D000818:Animals; D000906:Antibodies / Q000378:metabolism; D002417:Cattle; D003911:Dextrans / Q000737:chemistry*; D007163:Immunosorbent Techniques; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D011485:Protein Binding; D015203:Reproducibility of Results; D012710:Serum Albumin, Bovine / Q000302:isolation & purification / Q000378:metabolism; D013050:Spectrometry, Fluorescence
chemical_list: D000906:Antibodies; D003911:Dextrans; D058185:Magnetite Nanoparticles; D012710:Serum Albumin, Bovine; C014392:carboxymethyl dextran