title: Structural and biochemical characterization of the childhood cataract-associated R76S mutant of human γD-crystallin.
abstract: Although a number of γD-crystallin mutations are associated with cataract formation, there is not a clear understanding of the molecular mechanism(s) that lead to this protein deposition disease. As part of our ongoing studies on crystallins, we investigated the recently discovered Arg76 to Ser (R76S) mutation that is correlated with childhood cataract in an Indian family. We expressed the R76S γD-crystallin protein in E. coli, characterized it by CD, fluorescence, and NMR spectroscopy, and determined its stability with respect to thermal and chemical denaturation. Surprisingly, no significant biochemical or biophysical differences were observed between the wild-type protein and the R76S variant, except a lowered pI (6.8 compared to the wild-type value of 7.4). NMR assessment of the R76S γD-crystallin solution structure, by RDCs, and of its motional properties, by relaxation measurements, also revealed a close resemblance to wild-type crystallin. Further, kinetic unfolding/refolding experiments for R76S and wild-type protein showed similar degrees of off-pathway aggregation suppression by αB-crystallin. Overall, our results suggest that neither structural nor stability changes in the protein are responsible for the R76S γD-crystallin variant's association with cataract. However, the change in pI and the associated surface charge or the altered nature of the amino acid could influence interactions with other lens protein species.
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, Dalian, 116024, China.
authors.forename: Fangling
authors.initials: F
authors.lastname: Ji
authors.forename: Jinwon
authors.initials: J
authors.lastname: Jung
authors.forename: Angela M
authors.initials: AM
authors.lastname: Gronenborn
mesh_terms: D002386:Cataract / Q000235:genetics*; D002648:Child; D006801:Humans; D007700:Kinetics; D008958:Models, Molecular; D050505:Mutant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D055503:Protein Multimerization / Q000187:drug effects; D055550:Protein Stability / Q000187:drug effects; D020836:Protein Structure, Quaternary / Q000187:drug effects; D058767:Protein Unfolding / Q000187:drug effects; D012995:Solubility; D013816:Thermodynamics; D038203:alpha-Crystallin B Chain / Q000494:pharmacology; D038222:gamma-Crystallins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
chemical_list: C504903:CRYGD protein, human; D050505:Mutant Proteins; D038203:alpha-Crystallin B Chain; D038222:gamma-Crystallins