title: Influence of ligninolytic enzymes on straw saccharification during fungal pretreatment.
abstract: Solid state and submerged fermentations in the presence of white-rot basidiomycetes (Bjerkandera adusta, Fomes fomentarius, Ganoderma resinaceum, Irpex lacteus, Phanerochaete chrysosporium, Trametes versicolor and basidiomycete Euc-1) and the litter-decomposing basidiomycete Lepista nuda were evaluated as a pretreatment to increase enzymatic saccharification of wheat straw. Enzymatic hydrolysis of holocellulose after solid state pretreatment showed a significant (P<0.05) increase of saccharification process for T. versicolor, Euc-1, G. resinaceum and I. lacteus, being T. versicolor (strain Tv2) the best one with a sugar yield increase of 91% compared with untreated straw. In submerged medium the pretreatment with I. lacteus, Euc-1 and P. chrysosporium enhanced saccharification but at a lesser extent. Covariance analysis was used to evaluate the relationships between ligninolytic enzymes (lignin peroxidase, manganese-dependent peroxidase and laccase) and saccharification increase. Results showed that only the presence of lignin peroxidase during pretreatment can lead to a significant (P<0.05) increase in the saccharification yield.
authors.affiliation: CITAB - Universidade de Trás-os-Montes e Alto Douro, Department of Biology and Environment, Apartado 1013, 5001-801 Vila Real, Portugal.
authors.forename: Paula A
authors.initials: PA
authors.lastname: Pinto
authors.forename: Albino A
authors.initials: AA
authors.lastname: Dias
authors.forename: Irene
authors.initials: I
authors.lastname: Fraga
authors.forename: Guilhermina
authors.initials: G
authors.lastname: Marques
authors.forename: Miguel A M
authors.initials: MA
authors.lastname: Rodrigues
authors.forename: Jorge
authors.initials: J
authors.lastname: Colaço
authors.forename: Ana
authors.initials: A
authors.lastname: Sampaio
authors.forename: Rui M F
authors.initials: RM
authors.lastname: Bezerra
mesh_terms: D001487:Basidiomycota / Q000378:metabolism*; D004798:Enzymes / Q000378:metabolism*; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism*
chemical_list: D004798:Enzymes; D008031:Lignin