title: On-chip microelectrophoresis for the study of in vitro nonhomologous end-joining DNA double-strand break repair.
abstract: Oligomerization of linearized plasmids by nuclear proteins extracts, a recognized measure of nonhomologous end-joining (NHEJ) repair capacity, is typically assessed through agarose gel electrophoresis, a labor-intensive procedure. In the current study, a more convenient NHEJ assay was developed using microchips that allow scaled-down separation and quantification. This microchip method allows a considerable reduction in sample amount and analysis time with similar costs and comparable or slightly better precision. Data obtained with quercetin and wortmannin show that the method can be applied to the screening of food components and natural products for positive and negative modulators of NHEJ, potential chemopreventive and indirect genotoxic compounds, respectively.
authors.affiliation: Department of Therapeutic Chemistry and Pharmacognosy, Université de Mons, Belgium.
authors.forename: Catherine
authors.initials: C
authors.lastname: Charles
authors.forename: Moustapha
authors.initials: M
authors.lastname: Ouedraogo
authors.forename: Alexandra
authors.initials: A
authors.lastname: Belayew
authors.forename: Pierre
authors.initials: P
authors.lastname: Duez
mesh_terms: D000730:Androstadienes / Q000032:analysis; D004247:DNA / Q000737:chemistry*; D059766:DNA End-Joining Repair; D004260:DNA Repair; D004586:Electrophoresis / Q000295:instrumentation* / Q000379:methods; D011794:Quercetin / Q000032:analysis; D000077191:Wortmannin
chemical_list: D000730:Androstadienes; D004247:DNA; D011794:Quercetin; D000077191:Wortmannin