title: Use of endogenous NADH fluorescence for real-time in situ visualization of epicardial radiofrequency ablation lesions and gaps.
abstract: Radiofrequency ablation (RFA) aims to produce lesions that interrupt reentrant circuits or block the spread of electrical activation from sites of abnormal activity. Today, there are limited means for real-time visualization of cardiac muscle tissue injury during RFA procedures. We hypothesized that the fluorescence of endogenous NADH could be used as a marker of cardiac muscle injury during epicardial RFA procedures. Studies were conducted in blood-free and blood-perfused hearts from healthy adult Sprague-Dawley rats and New Zealand rabbits. Radiofrequency was applied to the epicardial surface of the heart using a 4-mm standard blazer ablation catheter. A dual camera optical mapping system was used to monitor NADH fluorescence upon ultraviolet illumination of the epicardial surface and to record optical action potentials using the voltage-sensitive probe RH237. Epicardial lesions were seen as areas of low NADH fluorescence. The lesions appeared immediately after ablation and remained stable for several hours. Real-time monitoring of NADH fluorescence allowed visualization of viable tissue between the RFA lesions. Dual recordings of NADH and epicardial electrical activity linked the gaps between lesions to postablation reentries. We found that the fluorescence of endogenous NADH aids the visualization of injured epicardial tissue caused by RFA. This was true for both blood-free and blood-perfused preparations. Gaps between NADH-negative regions revealed unablated tissue, which may promote postablation reentry or provide pathways for the conduction of abnormal electrical activity.
authors.affiliation: The George Washington University Medical Faculty Associates, Division of Cardiology, Washington, DC, USA.
authors.forename: Marco
authors.initials: M
authors.lastname: Mercader
authors.forename: Luther
authors.initials: L
authors.lastname: Swift
authors.forename: Sumit
authors.initials: S
authors.lastname: Sood
authors.forename: Huda
authors.initials: H
authors.lastname: Asfour
authors.forename: Matthew
authors.initials: M
authors.lastname: Kay
authors.forename: Narine
authors.initials: N
authors.lastname: Sarvazyan
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000818:Animals; D057791:Cardiac Imaging Techniques / Q000379:methods*; D017115:Catheter Ablation; D022062:Electrophysiologic Techniques, Cardiac / Q000379:methods; D005240:Feasibility Studies; D005453:Fluorescence; D023421:Models, Animal; D009243:NAD / Q000378:metabolism*; D009336:Necrosis; D010496:Pericardium / Q000378:metabolism* / Q000473:pathology* / Q000601:surgery; D011817:Rabbits; D051381:Rats
chemical_list: D009243:NAD