title: Norepinephrine suppresses IFN-γ and TNF-α production by murine intestinal intraepithelial lymphocytes via the β₁ adrenoceptor.
abstract: We examined whether norepinephrine (NE) had direct effects on cytokine production by murine intestinal intraepithelial lymphocytes (IELs), compared with splenocytes. CD3⁺ IELs and CD3⁺ splenocytes expressed α(1B), α(1D), α(2C), β₁, β₂, and β₃ adrenoceptors (ARs). NE significantly suppressed IFN-γ and TNF-α production by IELs and splenocytes ex vivo. The suppressive effects of NE in IELs were reversed by β₁ AR antagonist CGP-20712A, whereas those in splenocytes were reversed by β₂ AR antagonist ICI118,551. In IELs, β₁ AR agonist xamoterol mimicked the suppressive effects of NE. These results indicated NE regulates intestinal mucosal immune responses mediated by IELs via β₁ AR.
authors.affiliation: First Department of Medicine, Hamamatsu University School of Medicine, Hamamatsu 431-3192, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Takayanagi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Osawa
authors.forename: Mutsuhiro
authors.initials: M
authors.lastname: Ikuma
authors.forename: Kosuke
authors.initials: K
authors.lastname: Takagaki
authors.forename: Jiong
authors.initials: J
authors.lastname: Zhang
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Hamaya
authors.forename: Takanori
authors.initials: T
authors.lastname: Yamada
authors.forename: Mitsushige
authors.initials: M
authors.lastname: Sugimoto
authors.forename: Takahisa
authors.initials: T
authors.lastname: Furuta
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Miyajima
authors.forename: Ken
authors.initials: K
authors.lastname: Sugimoto
mesh_terms: D000818:Animals; D018928:Immunity, Mucosal / Q000276:immunology*; D007371:Interferon-gamma / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D007413:Intestinal Mucosa / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D016131:Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D061251:Primary Cell Culture; D018342:Receptors, Adrenergic, beta-1 / Q000502:physiology*; D013154:Spleen / Q000166:cytology / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors* / Q000096:biosynthesis
chemical_list: C547788:Adrb1 protein, mouse; D018342:Receptors, Adrenergic, beta-1; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma