title: 12-hydroxy-eicosatetraenoic acid (12-HETE): a biomarker of Churg-Strauss syndrome.
abstract: BACKGROUND
  Churg-Strauss syndrome (CSS) shares similarities with asthma and hypereosinophilic syndrome (HES). Eicosanoids--important inflammatory and signaling molecules--are present in exhaled breath condensate (EBC) and broncho-alveolar lavage fluid (BALF).
  
  
  OBJECTIVE
  To assess eicosanoid profile both in EBC and BALF of CSS subjects searching for a pattern characteristic of this syndrome.
  
  
  METHODS
  EBCs from 23 CSS patients, 30 asthmatics, 12 HES patients and 54 healthy controls (HC) were assessed quantitatively for 19 eicosanoids by a high-performance liquid chromatography - tandem mass spectrometry (HPLC-MS/MS). In addition, in 21 of 23 CSS subjects and in nine asthmatics, eicosanoids were determined in BALF.
  
  
  RESULTS
  EBC from CSS patients showed markedly elevated levels of 12-HETE as compared with other studied groups. BALF was characterized by a significant elevation of 12-HETE and its metabolite 12-tetranor HETE in CSS as compared with asthma. Clinical activity of CSS correlated with 12-HETE and its metabolites levels in BALF, but not in EBC.
  
  
  CONCLUSIONS
  CSS is clearly distinguished from bronchial asthma, and HES by a marked increase in 12-HETE concentration in both EBC and BALF. This points to a possible new pathogenic mechanism in CSS and may help in future in establishing the diagnosis of CSS.
authors.affiliation: Department of Medicine, Jagiellonian University Medical College, ul. Skawińska 8,Krakow, Poland. wszczeklik@gmail.com
authors.forename: W
authors.initials: W
authors.lastname: Szczeklik
authors.forename: M
authors.initials: M
authors.lastname: Sanak
authors.forename: L
authors.initials: L
authors.lastname: Mastalerz
authors.forename: B M
authors.initials: BM
authors.lastname: Sokołowska
authors.forename: A
authors.initials: A
authors.lastname: Gielicz
authors.forename: J
authors.initials: J
authors.lastname: Soja
authors.forename: J
authors.initials: J
authors.lastname: Kumik
authors.forename: J
authors.initials: J
authors.lastname: Musiał
authors.forename: A
authors.initials: A
authors.lastname: Szczeklik
mesh_terms: D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid / Q000032:analysis*; D000328:Adult; D015415:Biomarkers / Q000032:analysis*; D001944:Breath Tests; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D015267:Churg-Strauss Syndrome / Q000175:diagnosis* / Q000378:metabolism; D045853:Exhalation; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D053719:Tandem Mass Spectrometry
chemical_list: D015415:Biomarkers; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid