title: Methanotrophic communities of Saanich Inlet: a microcosm perspective.
abstract: Saanich Inlet (British Columbia, Canada) is a seasonally anoxic fjord characterized by high rates of both methane production and consumption. In this study, the diversity of microbial populations residing in intermediate waters, characterized by having a high methane content, was assessed using CH(4)-microcosm experiments coupled with PCR surveys of phylogenetic (16S rRNA gene) and functional gene markers (pmoA and fhcD genes). The experiments revealed that bacteria represented by sequences affiliated with Methylomicrobium within the Methylococcales, Methylophaga and Cycloclasticus within the Thiotrichales, and uncultured Planctomycetes were enriched in response to CH(4) addition.
authors.affiliation: Department of Microbiology, University of Washington, Seattle, WA 98195-1750, United States.
authors.forename: Linda M
authors.initials: LM
authors.lastname: Sauter
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Latypova
authors.forename: Nicole E
authors.initials: NE
authors.lastname: Smalley
authors.forename: Mary E
authors.initials: ME
authors.lastname: Lidstrom
authors.forename: Steven
authors.initials: S
authors.lastname: Hallam
authors.forename: Marina G
authors.initials: MG
authors.lastname: Kalyuzhnaya
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics; D058448:Biota; D001955:British Columbia; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D008697:Methane / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D008697:Methane