title: Rapid test for fecal calprotectin levels in children with Crohn disease.
abstract: Assessment of fecal calprotectin, a surrogate marker of mucosal inflammation, is a promising means to monitor therapeutic response in pediatric inflammatory bowel disease, especially if the result is readily available. We tested the performance of a novel calprotectin rapid test, Quantum Blue, versus the conventional enzyme-linked immunosorbent assay in 134 stool samples from 56 pediatric patients with Crohn disease. The intraclass correlation coefficient analysis reflected good agreement (intraclass correlation coefficient 0.97 [95% confidence interval 0.95-0.98]) but agreement was better in lower values, where dilutions were not required. Using a cutoff of 100 μg/g for normal values, the percentage agreement between the 2 tests was 87%. The optimal cutoff values to guide clinical decisions in the therapy of inflammatory bowel disease have yet to be determined.
authors.affiliation: Children's Hospital, Helsinki University Central Hospital, University of Helsinki, Helsinki, Finland. kaija-leena.kolho@helsinki.fi
authors.forename: K L
authors.initials: KL
authors.lastname: Kolho
authors.forename: D
authors.initials: D
authors.lastname: Turner
authors.forename: G
authors.initials: G
authors.lastname: Veereman-Wauters
authors.forename: M
authors.initials: M
authors.lastname: Sladek
authors.forename: L
authors.initials: L
authors.lastname: de Ridder
authors.forename: R
authors.initials: R
authors.lastname: Shaoul
authors.forename: A
authors.initials: A
authors.lastname: Paerregaard
authors.forename: J
authors.initials: J
authors.lastname: Amil Dias
authors.forename: S
authors.initials: S
authors.lastname: Koletzko
authors.forename: F
authors.initials: F
authors.lastname: Nuti
authors.forename: Y
authors.initials: Y
authors.lastname: Bujanover
authors.forename: A
authors.initials: A
authors.lastname: Staiano
authors.forename: K
authors.initials: K
authors.lastname: Bochenek
authors.forename: L
authors.initials: L
authors.lastname: Finnby
authors.forename: A
authors.initials: A
authors.lastname: Levine
authors.forename: G
authors.initials: G
authors.lastname: Veres
mesh_terms: D000293:Adolescent; D015415:Biomarkers / Q000032:analysis; D002648:Child; D002675:Child, Preschool; D016001:Confidence Intervals; D003424:Crohn Disease / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000737:chemistry*; D005260:Female; D006801:Humans; D007223:Infant; D007249:Inflammation / Q000378:metabolism*; D039841:Leukocyte L1 Antigen Complex / Q000032:analysis* / Q000378:metabolism; D008297:Male; D009092:Mucous Membrane / Q000378:metabolism; D012016:Reference Values; D015203:Reproducibility of Results
chemical_list: D015415:Biomarkers; D039841:Leukocyte L1 Antigen Complex