title: Effect of different levels of copper and molybdenum supplements on serum lipid profiles and antioxidant status in cashmere goats.
abstract: An experiment was conducted to investigate on the effects of different levels of copper (Cu: 0, 19, and 38 mg/kg) and molybdenum (Mo: 0 and 5 mg/kg) supplements and the interaction of these two factors on serum lipid profiles and antioxidant status in cashmere goats during the cashmere fiber growing period. Thirty-six Liaoning cashmere goats (approximately 1.5 years of age; 27.53±1.38 kg of body weight) were assigned to one of six treatments in a completely randomized design involving a 2×3 factorial arrangement. Goats were housed in individual pens and fed with Chinese wild rye- and alfalfa hay-based diet containing 4.72 mg Cu/kg, 0.16 mg Mo/kg, and 0.21 % S for 84 days. Blood samples were collected on day 84. The triglyceride concentration did not differ among treatments (P>0.05). Supplemental Cu, regardless of Mo level, decreased (P<0.05) the concentrations of serum total cholesterol and low density lipoprotein cholesterol, and increased (P<0.05) the concentration of serum high density lipoprotein cholesterol, but there were no differences (P>0.05) in these values between Cu-supplemented groups. Supplemental Cu increased (P<0.05) the activities of serum ceruloplasmin (Cp), Cu-zinc superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px), and decreased (P<0.05) the malondialdehyde content. The serum GSH-Px activity was also increased (P<0.05) by Mo supplementation. There was a tendency of the interaction effects of Cu and Mo on the activities of Cp (P=0.094), SOD (P=0.057), and GSH-Px (P=0.062), and goats fed with 19 mg Cu/kg in the absence of Mo tended to show the highest serum SOD activity, while goats fed with 38 mg Cu/kg with 5 mg Mo/kg tended to show the highest values of serum Cp and GSH-Px. Addition of Cu, Mo, or their interaction had no influence (P>0.05) on the activities of serum glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, and lactate dehydrogenase, and the concentrations of serum glucose and total protein. In conclusion, addition of 19 mg Cu/kg in the absence of Mo (the total dietary Cu level of 23.72 mg/kg) was recommended for altering the fat metabolism and obtaining the optimal antioxidant activity of cashmere goats, while 38 mg Cu/kg should be supplemented when 5 mg Mo/kg was added in the basal diet (the total dietary level of 42.72 mg Cu/kg, 5.16 mg Mo/kg, and 0.21 % S) during the cashmere growing period.
authors.affiliation: State Key Laboratory for Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Shi W
authors.initials: SW
authors.lastname: Zhang
authors.forename: Xian Z
authors.initials: XZ
authors.lastname: Song
authors.forename: Zhi H
authors.initials: ZH
authors.lastname: Jia
authors.forename: Run L
authors.initials: RL
authors.lastname: Wang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D003300:Copper / Q000097:blood*; D006041:Goats; D008055:Lipids / Q000097:blood*; D008982:Molybdenum / Q000097:blood*
chemical_list: D000975:Antioxidants; D008055:Lipids; D003300:Copper; D008982:Molybdenum