title: Mpg2 interacts and cooperates with Mpg1 to maintain yeast glycosylation.
abstract: Using a yeast two-hybrid screen, we isolated a gene from Schizosaccharomyces pombe, whose product interacts with Mpg1, a GDP-mannose-1-phosphate guanylyltransferase involved in the maintenance of cell wall integrity and glycosylation. We have designated this gene mpg2 based on its similarity to Mpg1. Mpg2 is evolutionarily conserved in higher eukaryotes. In the absence of Mpg2, defects in cell growth and sensitivity to hygromycin B are observed. When mpg1 is depleted, the lack of mpg2 causes a synthetic enhancement of the growth defect, the sensitivity to hygromycin B and the cell cycle phenotype previously reported for mpg1 mutant. Finally, Mpg1 overexpression complements the Δmpg2 mutant phenotypes. Taken together, these results indicate that mpg1 and mpg2 function together in glycosylation and septum formation.
authors.affiliation: Instituto de Hortofruticultura Subtropical y Mediterránea La Mayora, Universidad de Málaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Departamento de Biología Celular, Genética y Fisiología, Universidad de Málaga, Campus Teatinos, Málaga, Spain. mcmunoz@us.es
authors.forename: M Cruz
authors.initials: MC
authors.lastname: Muñoz-Centeno
authors.forename: Carlos
authors.initials: C
authors.lastname: Martín-Guevara
authors.forename: Amando
authors.initials: A
authors.lastname: Flores
authors.forename: Antonio J
authors.initials: AJ
authors.lastname: Pérez-Pulido
authors.forename: Cristina
authors.initials: C
authors.lastname: Antúnez-Rodríguez
authors.forename: Araceli G
authors.initials: AG
authors.lastname: Castillo
authors.forename: Miguel
authors.initials: M
authors.lastname: Sanchez-Durán
authors.forename: Pablo
authors.initials: P
authors.lastname: Mier
authors.forename: Eduardo R
authors.initials: ER
authors.lastname: Bejarano
mesh_terms: D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000378:metabolism; D002473:Cell Wall / Q000737:chemistry / Q000378:metabolism; D017353:Gene Deletion; D006921:Hygromycin B / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009713:Nucleotidyltransferases / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D011485:Protein Binding; D011487:Protein Conformation; D025941:Protein Interaction Mapping; D012568:Schizosaccharomyces / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D016415:Sequence Alignment; D020798:Two-Hybrid System Techniques
chemical_list: D000935:Antifungal Agents; D006921:Hygromycin B; D009713:Nucleotidyltransferases; C021546:mannose-1-phosphate guanylyltransferase (GDP)