title: Metabolite profiling of CHO cells with different growth characteristics.
abstract: Mammalian cell cultures are the predominant system for the production of recombinant proteins requiring post-translational modifications. As protein yields are a function of growth performance (among others), and performance varies greatly between culture medium (e.g., different growth rates and peak cell densities), an understanding of the biological mechanisms underpinning this variability would facilitate rational medium and process optimization, increasing product yields, and reducing costs. We employed a metabolomics approach to analyze differences in metabolite concentrations of CHO cells cultivated in three different media exhibiting different growth rates and maximum viable cell densities. Analysis of intra- and extracellular metabolite concentrations over the course of the cultures using a combination of HPLC and GC-MS, readily detected medium specific and time dependent changes. Using multivariate data analysis, we identified a range of metabolites correlating with growth rate, illustrating how metabolomics can be used to relate gross phenotypic changes to the fine details of cellular metabolism.
authors.affiliation: Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane, QLD 4072, Australia.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Dietmair
authors.forename: Mark P
authors.initials: MP
authors.lastname: Hodson
authors.forename: Lake-Ee
authors.initials: LE
authors.lastname: Quek
authors.forename: Nicholas E
authors.initials: NE
authors.lastname: Timmins
authors.forename: Panagiotis
authors.initials: P
authors.lastname: Chrysanthopoulos
authors.forename: Shana S
authors.initials: SS
authors.lastname: Jacob
authors.forename: Peter
authors.initials: P
authors.lastname: Gray
authors.forename: Lars K
authors.initials: LK
authors.lastname: Nielsen
mesh_terms: D000818:Animals; D016466:CHO Cells; D018929:Cell Culture Techniques / Q000379:methods; D006224:Cricetinae; D003412:Cricetulus; D003470:Culture Media / Q000737:chemistry; D004847:Epithelial Cells / Q000737:chemistry* / Q000378:metabolism*; D055442:Metabolome
chemical_list: D003470:Culture Media