title: Structure and orientation of the gH625-644 membrane interacting region of herpes simplex virus type 1 in a membrane mimetic system.
abstract: Glycoprotein H (gH) of the herpes simplex virus type 1 is involved in the complex mechanism of membrane fusion of the viral envelope with host cells. The virus requires four glycoproteins (gB, gD, gH, gL) to execute fusion and the role played by gH remains mysterious. Mutational studies have revealed several regions of gH ectodomain required for fusion and identified the segment from amino acid 625 to 644 as the most fusogenic region. Here, we studied the behavior in a membrane-mimicking DPC micellar environment of a peptide encompassing this region (gH625-644) and determined its NMR solution structure and its orientation within the micelles.
authors.affiliation: Department of Biological Sciences, Division of Biostructures, University of Naples Federico II, Napoli, Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: Galdiero
authors.forename: Luigi
authors.initials: L
authors.lastname: Russo
authors.forename: Annarita
authors.initials: A
authors.lastname: Falanga
authors.forename: Marco
authors.initials: M
authors.lastname: Cantisani
authors.forename: Mariateresa
authors.initials: M
authors.lastname: Vitiello
authors.forename: Roberto
authors.initials: R
authors.lastname: Fattorusso
authors.forename: Gaetano
authors.initials: G
authors.lastname: Malgieri
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Galdiero
authors.forename: Carla
authors.initials: C
authors.lastname: Isernia
mesh_terms: D032701:Biomimetics; D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism; D018259:Herpesvirus 1, Human / Q000378:metabolism*; D008823:Micelles; D019906:Nuclear Magnetic Resonance, Biomolecular; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D013329:Structure-Activity Relationship; D014759:Viral Envelope Proteins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D008823:Micelles; D010455:Peptides; D014759:Viral Envelope Proteins; C569201:gH625 peptide, Herpes simplex virus type I