title: ArgR of Streptomyces coelicolor is a versatile regulator.
abstract: ArgR is the regulator of arginine biosynthesis genes in Streptomyces species. Transcriptomic comparison by microarrays has been made between Streptomyces coelicolor M145 and its mutant S. coelicolor ΔargR under control, unsupplemented conditions, and in the presence of arginine. Expression of 459 genes was different in transcriptomic assays, but only 27 genes were affected by arginine supplementation. Arginine and pyrimidine biosynthesis genes were derepressed by the lack of ArgR, while no strong effect on expression resulted on arginine supplementation. Several nitrogen metabolism genes expression as glnK, glnA and glnII, were downregulated in S. coelicolor ΔargR. In addition, downregulation of genes for the yellow type I polyketide CPK antibiotic and for the antibiotic regulatory genes afsS and scbR was observed. The transcriptomic data were validated by either reverse transcription-PCR, expression of the gene-promoter coupled to the luciferase gene, proteomic or by electrophoresis mobility shift assay (EMSA) using pure Strep-tagged ArgR. Two ARG-boxes in the arginine operon genes suggest that these genes are more tightly controlled. Other genes, including genes encoding regulatory proteins, possess a DNA sequence formed by a single ARG-box which responds to ArgR, as validated by EMSA.
authors.affiliation: Área de Microbiología, Facultad de Ciencias Biológicas y Ambientales, Universidad de León, León, Spain.
authors.forename: Rosario
authors.initials: R
authors.lastname: Pérez-Redondo
authors.forename: Antonio
authors.initials: A
authors.lastname: Rodríguez-García
authors.forename: Alma
authors.initials: A
authors.lastname: Botas
authors.forename: Irene
authors.initials: I
authors.lastname: Santamarta
authors.forename: Juan F
authors.initials: JF
authors.lastname: Martín
authors.forename: Paloma
authors.initials: P
authors.lastname: Liras
mesh_terms: D001120:Arginine / Q000096:biosynthesis / Q000378:metabolism / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites; D001692:Biological Transport / Q000235:genetics; D004269:DNA, Bacterial / Q000096:biosynthesis; D009154:Mutation; D009584:Nitrogen / Q000378:metabolism; D009711:Nucleotides / Q000096:biosynthesis; D040901:Proteomics; D012329:RNA, Bacterial / Q000096:biosynthesis; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D048372:Streptomyces coelicolor / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects / Q000235:genetics; D059467:Transcriptome / Q000187:drug effects
chemical_list: C087018:ArgR protein, Bacteria; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D009711:Nucleotides; D012329:RNA, Bacterial; D012097:Repressor Proteins; D001120:Arginine; D009584:Nitrogen