title: Construction of a microstructured collagen membrane mimicking the papillary dermis architecture and guiding keratinocyte morphology and gene expression.
abstract: A papillary-structured collagen fibril membrane is created, mimicking the 3D-architecture of the human papillary dermis. Primary human keratinocytes cultured to confluency on papillar-structured films are compared to keratinocytes cultured on flat membranes. Microscopical evaluation reveals the presence of morphologically distinct cells at the base of the papillar structures that are not observed on flat membranes. Gene expression microarrays and RT-qPCR indicate that these cells are in a more proliferative/migrational state, whereas cells on flat membranes have a more differentiated expression profile. Immunohistochemical stainings confirm these results. In conclusion, specific collagen architecture can direct keratinocyte behavior, and this may be used to further improve skin regeneration.
authors.affiliation: Department of Biochemistry 280, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands.
authors.forename: Gerwen
authors.initials: G
authors.lastname: Lammers
authors.forename: Günter
authors.initials: G
authors.lastname: Roth
authors.forename: Mathias
authors.initials: M
authors.lastname: Heck
authors.forename: Roland
authors.initials: R
authors.lastname: Zengerle
authors.forename: G Sandra
authors.initials: GS
authors.lastname: Tjabringa
authors.forename: Elly M
authors.initials: EM
authors.lastname: Versteeg
authors.forename: Theo
authors.initials: T
authors.lastname: Hafmans
authors.forename: Ronnie
authors.initials: R
authors.lastname: Wismans
authors.forename: Dieter P
authors.initials: DP
authors.lastname: Reinhardt
authors.forename: Eugene T P
authors.initials: ET
authors.lastname: Verwiel
authors.forename: Patrick L J M
authors.initials: PL
authors.lastname: Zeeuwen
authors.forename: Joost
authors.initials: J
authors.lastname: Schalkwijk
authors.forename: Roland
authors.initials: R
authors.lastname: Brock
authors.forename: Willeke F
authors.initials: WF
authors.lastname: Daamen
authors.forename: Toin H
authors.initials: TH
authors.lastname: van Kuppevelt
mesh_terms: D040761:Biomimetic Materials / Q000737:chemistry* / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D003094:Collagen / Q000737:chemistry* / Q000494:pharmacology / Q000648:ultrastructure; D020405:Dermis / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects*; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D020411:Oligonucleotide Array Sequence Analysis; D061251:Primary Cell Culture; D054457:Tissue Scaffolds; D014945:Wound Healing / Q000502:physiology
chemical_list: D008567:Membranes, Artificial; D003094:Collagen