title: Early exercise affects mitochondrial transcription factors expression after cerebral ischemia in rats.
abstract: Increasing evidence shows that exercise training is neuroprotective after stroke, but the underlying mechanisms are unknown. To clarify this critical issue, the current study investigated the effects of early treadmill exercise on the expression of mitochondrial biogenesis factors. Adult rats were subjected to ischemia induced by middle cerebral artery occlusion followed by reperfusion. Expression of two genes critical for transcriptional regulation of mitochondrial biogenesis, peroxisome proliferator-activated receptor coactivator-1 (PGC-1) and nuclear respiratory factor-1 (NRF-1), were examined by RT-PCR after five days of exercise starting at 24 h after ischemia. Mitochondrial protein cytochrome C oxidase subunit IV (COX IV) was detected by Western blot. Neurological status and cerebral infarct volume were evaluated as indices of brain damage. Treadmill training increased levels of PGC-1 and NRF-1 mRNA, indicating that exercise promotes rehabilitation after ischemia via regulation of mitochondrial biogenesis.
authors.affiliation: Department of Rehabilitation, Huashan Hospital, Fudan University, Shanghai 200040, China.|Department of Sports Medicine and Rehabilitation, Medical College of Fudan University, Shanghai 200032, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Rehabilitation, Huashan Hospital, Fudan University, Shanghai 200040, China.|Department of Sports Medicine and Rehabilitation, Medical College of Fudan University, Shanghai 200032, China.|The Yonghe Branch of Huashan Hospital, Fudan University, Shanghai 200436, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai 200032, China.
authors.forename: Hongying
authors.initials: H
authors.lastname: Sha
authors.affiliation: Department of Rehabilitation, Huashan Hospital, Fudan University, Shanghai 200040, China.|Department of Sports Medicine and Rehabilitation, Medical College of Fudan University, Shanghai 200032, China.
authors.forename: Pengyue
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Department of Rehabilitation, Huashan Hospital, Fudan University, Shanghai 200040, China.|Department of Sports Medicine and Rehabilitation, Medical College of Fudan University, Shanghai 200032, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Jia
authors.affiliation: Department of Rehabilitation, Huashan Hospital, Fudan University, Shanghai 200040, China.|Department of Sports Medicine and Rehabilitation, Medical College of Fudan University, Shanghai 200032, China.|The Yonghe Branch of Huashan Hospital, Fudan University, Shanghai 200436, China.
authors.forename: Yongshan
authors.initials: Y
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai 200032, China.|Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai 200040, China.
authors.forename: Jianhong
authors.initials: J
authors.lastname: Zhu
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000235:genetics* / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism; D005786:Gene Expression Regulation; D020244:Infarction, Middle Cerebral Artery / Q000150:complications / Q000235:genetics / Q000378:metabolism; D008297:Male; D008928:Mitochondria / Q000235:genetics* / Q000378:metabolism; D051266:Nuclear Respiratory Factor 1 / Q000235:genetics / Q000378:metabolism; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D010805:Physical Conditioning, Animal / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D051266:Nuclear Respiratory Factor 1; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; C501429:Ppargc1a protein, rat; D014157:Transcription Factors; D003576:Electron Transport Complex IV