title: Reactive oxygen species-driven transcription in Arabidopsis under oxygen deprivation.
abstract: Reactive oxygen species (ROS) play an important role as triggers of gene expression during biotic and abiotic stresses, among which is low oxygen (O(2)). Previous studies have shown that ROS regulation under low O(2) is driven by a RHO-like GTPase that allows tight control of hydrogen peroxide (H(2)O(2)) production. H(2)O(2) is thought to regulate the expression of heat shock proteins, in a mechanism that is common to both O(2) deprivation and to heat stress. In this work, we used publicly available Arabidopsis (Arabidopsis thaliana) microarray datasets related to ROS and O(2) deprivation to define transcriptome convergence pattern. Our results show that although Arabidopsis response to anoxic and hypoxic treatments share a common core of genes related to the anaerobic metabolism, they differ in terms of ROS-related gene response. We propose that H(2)O(2) production under O(2) deprivation is a trait present in a very early phase of anoxia, and that ROS are needed for the regulation of a set of genes belonging to the heat shock protein and ROS-mediated groups. This mechanism, likely not regulated via the N-end rule pathway for O(2) sensing, is probably mediated by a NADPH oxidase and it is involved in plant tolerance to the stress.
authors.affiliation: PlantLab, Institute of Life Sciences, Scuola Superiore Sant'Anna, 56127 Pisa, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Pucciariello
authors.forename: Sandro
authors.initials: S
authors.lastname: Parlanti
authors.forename: Valeria
authors.initials: V
authors.lastname: Banti
authors.forename: Giacomo
authors.initials: G
authors.lastname: Novi
authors.forename: Pierdomenico
authors.initials: P
authors.lastname: Perata
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D015687:Cell Hypoxia; D003470:Culture Media / Q000378:metabolism; D016208:Databases, Factual; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018869:Heat-Shock Response; D006358:Hot Temperature; D006861:Hydrogen Peroxide / Q000378:metabolism; D019255:NADPH Oxidases / Q000235:genetics / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D036226:Seedlings / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D013997:Time Factors; D014158:Transcription, Genetic
chemical_list: D003470:Culture Media; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D019255:NADPH Oxidases; D010100:Oxygen