title: Petroleum and polycyclic aromatic hydrocarbons (PAHs) degradation and naphthalene metabolism in Streptomyces sp. (ERI-CPDA-1) isolated from oil contaminated soil.
abstract: Petroleum and polycyclic aromatic hydrocarbons (PAHs) degrading Streptomyces sp. isolate ERI-CPDA-1 was recovered from oil contaminated soil in Chennai, India. The degradation efficiencies were examined by GC-FID and the results showed that the isolate could remove 98.25% diesel oil, 99.14% naphthalene and 17.5% phenanthrene in 7 days at 30°C (0.1%). ERI-CPDA-1 was able to degrade naphthalene, phenanthrene and diesel oil and grow on petrol, diesel, kerosene, benzene, pyridine, methanol, ethanol, cyclohexane, tween-80, xylene, DMSO and toluene using them as sole carbon source. Effects of environmental condition on degradation of hydrocarbons (diesel oil, naphthalene and phenanthrene) were also studied at different pH, NaCl, temperature, concentration and incubation time. Degradation pathway for naphthalene has been proposed. Degradation metabolites were identified using GC-MS analysis of ethyl acetate extract of the cell free culture. The degradation products detected were benzaldehyde, catechol, phenylacetic acid and protocatechuic acid.
authors.affiliation: Division of Microbiology, Entomology Research Institute, Loyola College, Nungambakkam, Chennai 600 034, Tamil Nadu, India.
authors.forename: C
authors.initials: C
authors.lastname: Balachandran
authors.forename: V
authors.initials: V
authors.lastname: Duraipandiyan
authors.forename: K
authors.initials: K
authors.lastname: Balakrishna
authors.forename: S
authors.initials: S
authors.lastname: Ignacimuthu
mesh_terms: D001673:Biodegradation, Environmental / Q000187:drug effects; D018533:Biomass; D002244:Carbon / Q000494:pharmacology; D053858:Metabolic Networks and Pathways / Q000187:drug effects; D008969:Molecular Sequence Data; D009281:Naphthalenes / Q000378:metabolism*; D009821:Oils / Q000737:chemistry; D010578:Petroleum / Q000382:microbiology*; D010616:Phenanthrenes / Q000378:metabolism; D010802:Phylogeny; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis*; D013302:Streptomyces / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism*; D013379:Substrate Specificity / Q000187:drug effects
chemical_list: D009281:Naphthalenes; D009821:Oils; D010578:Petroleum; D010616:Phenanthrenes; D011084:Polycyclic Aromatic Hydrocarbons; D012989:Soil Pollutants; C031721:naphthalene; C031181:phenanthrene; D002244:Carbon