title: Proliferation of equine bone marrow-derived mesenchymal stem cells in gelatin/β-tricalcium phosphate sponges.
abstract: A three dimensional scaffold is essential in mesenchymal stem cells (MSCs) delivery in cell-based therapy for facilitating cell adherence, migration, proliferation, and differentiation. The objectives of this study were to evaluate the possibility of β-tricalcium phosphate incorporated gelatin sponges (Gelatin/β-TCP sponge) as scaffolds for equine MSCs and to examine the effects of seeding density and seeding method on the proliferation of equine MSCs in the Gelatin/β-TCP sponges. Mononuclear cells and MSCs isolated from bone marrow were seeded into Gelatin/β-TCP sponges at different densities by different seeding methods-static or agitated methods. Proliferation of the MSCs in Gelatin/β-TCP was assessed using the Cell Counting Kit-8 assay and histological examination. Distribution and proliferation of MSCs in the Gelatin/β-TCP sponge were observed, and the Gelatin/β-TCP sponge supported limited growth when seeded at high density. We also found that the agitated seeding method enhanced the proliferation of MSCs. This study demonstrated the suitability of Gelatin/β-TCP sponges for the proliferation and maintenance of equine MSCs. These results contribute to the application of MSC-seeded Gelatin/β-TCP sponges in equine medicine.
authors.affiliation: Department of Clinical Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro-city, Hokkaido 080-8555, Japan.
authors.forename: Jong-pil
authors.initials: JP
authors.lastname: Seo
authors.forename: Nao
authors.initials: N
authors.lastname: Tsuzuki
authors.forename: Shingo
authors.initials: S
authors.lastname: Haneda
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Yamada
authors.forename: Hidefumi
authors.initials: H
authors.lastname: Furuoka
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Tabata
authors.forename: Naoki
authors.initials: N
authors.lastname: Sasaki
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002130:Calcium Phosphates / Q000737:chemistry*; D049109:Cell Proliferation; D005260:Female; D005780:Gelatin / Q000737:chemistry*; D006736:Horses; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D023822:Tissue Engineering / Q000379:methods / Q000662:veterinary*; D054457:Tissue Scaffolds / Q000662:veterinary*
chemical_list: D002130:Calcium Phosphates; D005780:Gelatin; C018392:tricalcium phosphate