title: A portable and integrated nucleic acid amplification microfluidic chip for identifying bacteria.
abstract: In this work, we developed a portable integrated microchip of loop-mediated isothermal nucleic acid amplification (LAMP). This chip, with sample-to-answer capability, could perform rapid DNA release, exponential signal amplification and naked-eye result read-out in single or multiplex format. We call it iμLAMP, namely integrated micro-LAMP, which was successfully used for point-of-care identification of bacteria.
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, PR China.
authors.forename: Xueen
authors.initials: X
authors.lastname: Fang
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.forename: Lingjia
authors.initials: L
authors.lastname: Xu
authors.forename: Xingyu
authors.initials: X
authors.lastname: Jiang
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Wu
authors.forename: Jilie
authors.initials: J
authors.lastname: Kong
mesh_terms: D001419:Bacteria / Q000235:genetics* / Q000302:isolation & purification*; D001424:Bacterial Infections / Q000175:diagnosis; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification; D004867:Equipment Design; D006801:Humans; D046210:Microfluidic Analytical Techniques; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification; D021141:Nucleic Acid Amplification Techniques / Q000295:instrumentation*; D019095:Point-of-Care Systems
chemical_list: D004269:DNA, Bacterial