title: Reproducibility and accuracy of non-invasive measurement of infarct size in mice with high-resolution PET/CT.
abstract: BACKGROUND
  This study assessed the reproducibility and accuracy of 2-deoxy-2[(18)F]fluoro-D-glucose ((18)F-FDG) for non-invasive quantification of myocardial infarct size in mice by a high-resolution positron emission tomography (PET)/computed tomography (CT) system.
  
  
  RESULTS
  Mice were studied by (18)F-FDG PET/CT 1 week after induction of myocardial infarction by permanent coronary occlusion or sham procedure. In a subset of mice, PET/CT was repeated 2 days apart to assess the reproducibility of infarct size measurements. Histological analysis was used as reference method to validate imaging data. The average difference in infarct size measurements between the first and the second study was -0.42% ± 2.07% (95% confidence interval -2.6 to 1.75) with a repeatability coefficient of 4.05%. At Bland-Altman analysis, the lower and upper limits of agreement between the two repeated studies were -4.46% and 3.63%, respectively, and no correlation between difference and mean was found (P = .89). The concordance correlation coefficient was 0.99 (P < .001) and the intraclass coefficient of correlation was 0.99. A high correlation between PET/CT and histology was found for measurement of infarct size (P < .001). Using Bland-Altman analysis, the mean difference in infarct size measurement (PET/CT minus histology) was 1.9% (95% confidence interval 0.94% to 2.86%).
  
  
  CONCLUSIONS
  In a mice model of permanent coronary occlusion non-invasive measurement of infarct size with high-resolution (18)F-FDG, PET/CT has excellent reproducibility and accuracy. These findings support the use of this methodology in serial studies.
authors.affiliation: Department of Biomorphological and Functional Sciences, University Federico II, Naples, Italy.
authors.forename: Adelaide
authors.initials: A
authors.lastname: Greco
authors.forename: Maria Piera
authors.initials: MP
authors.lastname: Petretta
authors.forename: Michele
authors.initials: M
authors.lastname: Larobina
authors.forename: Sara
authors.initials: S
authors.lastname: Gargiulo
authors.forename: Mariarosaria
authors.initials: M
authors.lastname: Panico
authors.forename: Stephan G
authors.initials: SG
authors.lastname: Nekolla
authors.forename: Giovanni
authors.initials: G
authors.lastname: Esposito
authors.forename: Mario
authors.initials: M
authors.lastname: Petretta
authors.forename: Arturo
authors.initials: A
authors.lastname: Brunetti
authors.forename: Alberto
authors.initials: A
authors.lastname: Cuocolo
mesh_terms: D000465:Algorithms; D000818:Animals; D005260:Female; D019788:Fluorodeoxyglucose F18; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D064847:Multimodal Imaging / Q000662:veterinary*; D009203:Myocardial Infarction / Q000175:diagnosis*; D010363:Pattern Recognition, Automated / Q000379:methods*; D049268:Positron-Emission Tomography; D019275:Radiopharmaceuticals; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014057:Tomography, X-Ray Computed
chemical_list: D019275:Radiopharmaceuticals; D019788:Fluorodeoxyglucose F18