title: Crystal structures of putative phosphoglycerate kinases from B. anthracis and C. jejuni.
abstract: Phosphoglycerate kinase (PGK) is indispensable during glycolysis for anaerobic glucose degradation and energy generation. Here we present comprehensive structure analysis of two putative PGKs from Bacillus anthracis str. Sterne and Campylobacter jejuni in the context of their structural homologs. They are the first PGKs from pathogenic bacteria reported in the Protein Data Bank. The crystal structure of PGK from Bacillus anthracis str. Sterne (BaPGK) has been determined at 1.68 Å while the structure of PGK from Campylobacter jejuni (CjPGK) has been determined at 2.14 Å resolution. The proteins' monomers are composed of two domains, each containing a Rossmann fold, hinged together by a helix which can be used to adjust the relative position between two domains. It is also shown that apo-forms of both BaPGK and CjPGK adopt open conformations as compared to the substrate and ATP bound forms of PGK from other species.
authors.affiliation: Department of Molecular Physiology and Biological Physics, University of Virginia, 1340 Jefferson Park Avenue, Charlottesville, VA 22908, USA.
authors.forename: Heping
authors.initials: H
authors.lastname: Zheng
authors.forename: Ekaterina V
authors.initials: EV
authors.lastname: Filippova
authors.forename: Karolina L
authors.initials: KL
authors.lastname: Tkaczuk
authors.forename: Piotr
authors.initials: P
authors.lastname: Dworzynski
authors.forename: Maksymilian
authors.initials: M
authors.lastname: Chruszcz
authors.forename: Przemyslaw J
authors.initials: PJ
authors.lastname: Porebski
authors.forename: Zdzislaw
authors.initials: Z
authors.lastname: Wawrzak
authors.forename: Olena
authors.initials: O
authors.lastname: Onopriyenko
authors.forename: Marina
authors.initials: M
authors.lastname: Kudritska
authors.forename: Sarah
authors.initials: S
authors.lastname: Grimshaw
authors.forename: Alexei
authors.initials: A
authors.lastname: Savchenko
authors.forename: Wayne F
authors.initials: WF
authors.lastname: Anderson
authors.forename: Wladek
authors.initials: W
authors.lastname: Minor
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry; D001051:Apoenzymes / Q000737:chemistry; D001408:Bacillus anthracis / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry*; D016123:Campylobacter jejuni / Q000201:enzymology*; D018360:Crystallography, X-Ray; D010735:Phosphoglycerate Kinase / Q000737:chemistry*; D017510:Protein Folding; D017434:Protein Structure, Tertiary
chemical_list: D001051:Apoenzymes; D001426:Bacterial Proteins; D000255:Adenosine Triphosphate; D010735:Phosphoglycerate Kinase