title: Comparative antifungal activity of cilofungin (LY121019) against Candida species, including evaluation of susceptibility testing method.
abstract: The in vitro activity of cilofungin against 100 Candida species was compared with 5-flucytosine. amphotericin B and ketoconazole by two laboratories independently and in a blinded fashion using a macrotitre dilution broth method in saam-f medium. Cilofungin showed good in vitro activity against Candida albicans. Candida tropicalis and Candida glabrata (90% minimal inhibitory concentration [MIC] 3.2 μg/mL) but was inactive against other Candida species. When testing the susceptibility of cilofungin, 5-flucytosine and amphotericin B at the two centres, approximately 90% of the Candida strains had MICs differing by fourfold or less. However, when testing susceptibility of ketoconazole, only 51% of the Candida strains had MIC differences fourfold or less. MIC susceptibility testing with cilofungin, 5-flucytosine and amphotericin B in saam-f medium is reproducible.
authors.affiliation: Departments of Microbiology, Mount Sinai Hospital, University of Toronto, Toronto, Ontario; and Health Sciences Centre, University of Manitoba, Winnipeg, Manitoba.
authors.forename: A H
authors.initials: AH
authors.lastname: Chagla
authors.forename: J H
authors.initials: JH
authors.lastname: Hii
authors.forename: D J
authors.initials: DJ
authors.lastname: Hoban
authors.forename: A E
authors.initials: AE
authors.lastname: Simor
authors.forename: S
authors.initials: S
authors.lastname: Ferro
authors.forename: E
authors.initials: E
authors.lastname: Witwicki
authors.forename: R
authors.initials: R
authors.lastname: Poon
authors.forename: D E
authors.initials: DE
authors.lastname: Low