title: Role of ErbB2 mediated AKT and MAPK pathway in gall bladder cell proliferation induced by argemone oil and butter yellow. Argemone oil and butter yellow induced gall bladder cell proliferation.
abstract: The effect of noncytotoxic doses of argemone oil (AO) and butter yellow (BY), the common adulterants in edible oil, on free radical generation and signaling pathway for cell proliferation in primary cells of gall bladder (GB) was undertaken. AO and BY showed no cytotoxicity at 0.1 μl/ml and 0.1 μg/ml concentration, respectively. AO caused significant increase in ROS after 30 min and RNS after 24 h in GB cells while no change was observed following BY treatment. Enhanced level of COX-2 was observed following AO (0.1 μl/ml) and BY (0.1 μg/ml) treatment to cells for 24 h. AO treatment caused phosphorylation of ErbB2, AKT, ERK, and JNK along with increased thymidine uptake indicating cell proliferation ability in GB cells. BY treatment also showed significant expression of these proteins with the exception of phosphorylated JNK. These results suggest that AO and BY have cell proliferative potential in GB cells following up-regulation of COX-2 and ErbB2; however, their downstream signaling molecules and free radical generation have differential response, indicating that the mechanism of proliferation is different for both compounds and may have relevance in gall bladder cancer.
authors.affiliation: Food Drug & Chemical Toxicology Group, CSIR-Indian Institute of Toxicology Research, Council of Scientific and Industrial Research, Post Box#80, Lucknow, 226 001, India.
authors.forename: Vivek
authors.initials: V
authors.lastname: Mishra
authors.forename: Kausar M
authors.initials: KM
authors.lastname: Ansari
authors.forename: Raj
authors.initials: R
authors.lastname: Khanna
authors.forename: Mukul
authors.initials: M
authors.lastname: Das
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D049109:Cell Proliferation; D003470:Culture Media / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D004121:Dimethyl Sulfoxide / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005704:Gallbladder / Q000187:drug effects* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D020935:MAP Kinase Signaling System; D051379:Mice; D010766:Phosphorylation; D010938:Plant Oils / Q000633:toxicity*; D061251:Primary Cell Culture; D026361:Reactive Nitrogen Species / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D018675:Toxicity Tests / Q000379:methods; D004124:p-Dimethylaminoazobenzene / Q000633:toxicity*
chemical_list: D003470:Culture Media; D051546:Cyclooxygenase 2; D004121:Dimethyl Sulfoxide; D010938:Plant Oils; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; D018719:Erb-b2 Receptor Tyrosine Kinases; D013936:Thymidine; D004124:p-Dimethylaminoazobenzene; C030610:argemone oil; C077623:Ptgs2 protein, mouse; C524056:Erbb2 protein, mouse