title: Presence of CP4-EPSPS component in roundup ready soybean-derived food products.
abstract: With the widespread use of Roundup Ready soya (event 40-3-2) (RRS), the traceability of transgenic components, especially protein residues, in different soya-related foodstuffs has become an important issue. In this report, transgenic components in commercial soya (including RRS) protein concentrates were firstly detected by using polymerase chain reaction (PCR) and western blot. The results illustrated the different degradation patterns of the cp4-epsps gene and corresponding protein in RRS-derived protein concentrates. Furthermore, western blot was applied to investigate the single factor of food processing and the matrix on the disintegration of CP4-EPSPS protein in RRS powder and soya-derived foodstuffs, and trace the degradation patterns during the food production chain. Our results suggested that the exogenous full length of CP4-EPSPS protein in RRS powder was distinctively sensitive to various heat treatments, including heat, microwave and autoclave (especially), and only one degradation fragment (23.4 kD) of CP4-EPSPS protein was apparently observed when autoclaving was applied. By tracing the protein degradation during RRS-related products, including tofu, tou-kan, and bean curd sheets, however, four degradation fragments (42.9, 38.2, 32.2 and 23.4 kD) were displayed, suggesting that both boiling and bittern adding procedures might have extensive effects on CP4-EPSPS protein degradation. Our results thus confirmed that the distinctive residues of the CP4-EPSPS component could be traced in RRS-related foodstuffs.
authors.affiliation: College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.|College of Food Sciences, National Center of Meat Quality and Safety Control, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Honghong
authors.initials: H
authors.lastname: Wu
authors.affiliation: Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore 637371, Singapore.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Jiangsu Entry-Exit Inspection and Quarantine Bureau, Nanjing 210001, China.
authors.forename: Changqing
authors.initials: C
authors.lastname: Zhu
authors.affiliation: College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Xiao
authors.affiliation: College of Food Sciences, National Center of Meat Quality and Safety Control, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Xinghu
authors.initials: X
authors.lastname: Zhou
authors.affiliation: College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Xu
authors.affiliation: College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Wenbiao
authors.identifier: 0000-0003-1525-9472
authors.initials: W
authors.lastname: Shen
authors.affiliation: College of Food Sciences, National Center of Meat Quality and Safety Control, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Huang
mesh_terms: D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D060054:Agrobacterium / Q000201:enzymology* / Q000235:genetics; D018556:Crops, Agricultural; D005506:Food Contamination / Q000032:analysis; D005511:Food Handling; D030821:Plants, Genetically Modified; D059748:Proteolysis; D045730:Soy Foods / Q000032:analysis*; D030262:Soybean Proteins / Q000737:chemistry; D013025:Glycine max / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D030262:Soybean Proteins; D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase