title: Analysis of the association of an MMP1 promoter polymorphism and transcript levels with chronic periodontitis and end-stage renal disease in a Brazilian population.
abstract: Chronic periodontitis (CP) and end-stage renal disease (ESRD) are complex inflammatory conditions. Higher levels of MMP-1 were found in fluids and gingival tissues from CP patients and in the blood and tissues from ESRD patients. MMP1-1607 (1G/2G) is a functional polymorphism, as it alters MMP-1 expression.
  
  
  OBJECTIVE
  The aim of this study was to investigate the association of the MMP1-1607 (1G/2G) polymorphism with CP and ESRD and evaluate differences in transcript levels between the groups.
  
  
  METHODS
  A total of 254 individuals were divided into four groups: Group 1, without CP and without chronic kidney disease (CKD) (n = 67); Group 2, with CP and without CKD (n = 60); Group 3, without CP and with CKD stage 5 (ESRD) (n = 52), and Group 4, with CP and with ESRD (n = 75). The MMP1-1607 polymorphism was analysed by PCR-RFLP. MMP1 gene transcripts from gingival tissues were analysed by real-time PCR.
  
  
  RESULTS
  No association was found between the MMP1-1607 polymorphism and CP or ESRD. Increased levels of MMP1 transcripts were observed in CP patients with or without ESRD. No differences were observed in the transcript levels according to the genotypes.
  
  
  CONCLUSIONS
  It was concluded that the MMP1-1607 polymorphism was not associated with either CP or ESRD. However, higher levels of MMP1 gene transcripts were found at gingival sites of CP in patients both with and without ESRD.
authors.affiliation: Center for Health and Biological Sciences at Pontifícia Universidade Católica do Paraná (PUCPR), Curitiba, PR, Brazil.
authors.forename: Sônia M
authors.initials: SM
authors.lastname: Luczyszyn
authors.forename: Cleber M
authors.initials: CM
authors.lastname: de Souza
authors.forename: Ana P R
authors.initials: AP
authors.lastname: Braosi
authors.forename: Acir J
authors.initials: AJ
authors.lastname: Dirschnabel
authors.forename: Marcela
authors.initials: M
authors.lastname: Claudino
authors.forename: Carlos E
authors.initials: CE
authors.lastname: Repeke
authors.forename: Fábio R
authors.initials: FR
authors.lastname: Faucz
authors.forename: Gustavo P
authors.initials: GP
authors.lastname: Garlet
authors.forename: Roberto
authors.initials: R
authors.lastname: Pecoits-Filho
authors.forename: Paula C
authors.initials: PC
authors.lastname: Trevilatto
mesh_terms: D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D001938:Brazil; D055113:Chronic Periodontitis / Q000235:genetics*; D004847:Epithelial Cells / Q000502:physiology*; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000235:genetics*; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000235:genetics*; D008875:Middle Aged; D009061:Mouth Mucosa / Q000502:physiology*; D012150:Polymorphism, Restriction Fragment Length; D011401:Promoter Regions, Genetic; D060888:Real-Time Polymerase Chain Reaction; D018709:Statistics, Nonparametric
chemical_list: D020781:Matrix Metalloproteinase 1