title: Functional diversity of bitter taste receptor TAS2R16 in primates.
abstract: In mammals, bitter taste is mediated by TAS2R genes, which belong to the large family of seven transmembrane G protein-coupled receptors. Because TAS2Rs are directly involved in the interaction between mammals and their dietary sources, it is likely that these genes evolved to reflect species-specific diets during mammalian evolution. Here, we investigated the sensitivities of TAS2R16s of various primates by using a cultured cell expression system, and found that the sensitivity of each primate species varied according to the ligand. Especially, the sensitivity of TAS2R16 of Japanese macaques to salicin was much lower than that of human TAS2R16, which was supported by behavioural tests. These results suggest the possibility that bitter-taste sensitivities evolved independently by replacing specific amino acid residues of TAS2Rs in different primate species to adapt to food items they use.
authors.affiliation: Primate Research Institute, Kyoto University, Inuyama, Aichi 484-8506, Japan. imai@pri.kyoto-u.ac.jp
authors.forename: Hiroo
authors.initials: H
authors.lastname: Imai
authors.forename: Nami
authors.initials: N
authors.lastname: Suzuki
authors.forename: Yoshiro
authors.initials: Y
authors.lastname: Ishimaru
authors.forename: Takanobu
authors.initials: T
authors.lastname: Sakurai
authors.forename: Lijie
authors.initials: L
authors.lastname: Yin
authors.forename: Wenshi
authors.initials: W
authors.lastname: Pan
authors.forename: Keiko
authors.initials: K
authors.lastname: Abe
authors.forename: Takumi
authors.initials: T
authors.lastname: Misaka
authors.forename: Hirohisa
authors.initials: H
authors.lastname: Hirai
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001522:Behavior, Animal; D001592:Benzyl Alcohols / Q000378:metabolism; D001665:Binding Sites; D004247:DNA / Q000097:blood; D019143:Evolution, Molecular; D005796:Genes; D005960:Glucosides / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D008251:Macaca / Q000235:genetics* / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000235:genetics* / Q000378:metabolism; D013045:Species Specificity; D013649:Taste / Q000235:genetics*; D055697:Taste Perception; D014162:Transfection; D000098706:Taste Receptors, Type 2
chemical_list: D001592:Benzyl Alcohols; D005960:Glucosides; D043562:Receptors, G-Protein-Coupled; D000098706:Taste Receptors, Type 2; C005696:salicin; D004247:DNA