title: Fabrication and characterization of poly(L-lactide-co-glycolide) knitted mesh-reinforced collagen-chitosan hybrid scaffolds for dermal tissue engineering.
abstract: Mechanical properties are essential considerations for the design of porous scaffolds in the field of tissue engineering. To develop a well-supported hybrid dermal substitute, poly(L-lactide-co-glycolide) (PLGA) yarns were knitted into a mesh with relative fixed loops, followed by incorporation into collagen-chitosan scaffolds (CCS) to obtain PLGA knitted mesh-reinforced CCS (PLGAm/CCS). The morphology and tensile strength in both the dry and wet state of PLGAm/CCS were investigated in vitro. To characterize the tissue response, specifically angiogenesis and tissue regeneration, PLGAm/CCS was embedded subcutaneously in Sprague-Dawley rats and compared with two control implants, i.e., PLGA mesh (PLGAm) and CCS. At weeks 1, 2, and 4 post surgery, tissue specimens were harvested for histology, immunohistochemistry, real-time quantitative PCR and Western blot analysis. These results demonstrated that the incorporation of PLGA knitted mesh into CCS can improve the mechanical strength with little influence on its mean pore size and porosity. After implantation, PLGAm/CCS can resist contraction and promote cell infiltration, neotissue formation, and blood vessel ingrowth, effectively. In conclusion, the mechanical strength of scaffolds can play a synergetic role in tissue regeneration and vascularization by maintaining its 3D microstructure. The ability of PLGAm/CCS to promote angiogenesis and induce in situ tissue formation demonstrates its strong potential in the field of skin tissue engineering.
authors.affiliation: Department of Burns, Second Affiliated Hospital of Zhejiang University, College of Medicine, Hangzhou, 310009, China.
authors.forename: Xingang
authors.initials: X
authors.lastname: Wang
authors.forename: Qiyin
authors.initials: Q
authors.lastname: Li
authors.forename: Xinlei
authors.initials: X
authors.lastname: Hu
authors.forename: Lie
authors.initials: L
authors.lastname: Ma
authors.forename: Chuangang
authors.initials: C
authors.lastname: You
authors.forename: Yurong
authors.initials: Y
authors.lastname: Zheng
authors.forename: Huafeng
authors.initials: H
authors.lastname: Sun
authors.forename: Chunmao
authors.initials: C
authors.lastname: Han
authors.forename: Changyou
authors.initials: C
authors.lastname: Gao
mesh_terms: D000818:Animals; D002417:Cattle; D048271:Chitosan / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry*; D005612:Freeze Drying; D019344:Lactic Acid / Q000737:chemistry*; D008297:Male; D008422:Materials Testing; D055595:Mechanical Phenomena; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D016133:Polymerase Chain Reaction; D051381:Rats; D012867:Skin / Q000166:cytology* / Q000378:metabolism; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D003094:Collagen; D048271:Chitosan