title: Inhibition of neutrophil elastase and metalloprotease-9 of human adenocarcinoma gastric cells by chamomile (Matricaria recutita L.) infusion.
abstract: This study investigated whether the antiinflammatory effect of chamomile infusion at gastric level could be ascribed to the inhibition of metalloproteinase-9 and elastase. The infusions from capitula and sifted flowers (250-1500 µg/mL) and individual flavonoids (10 µM) were tested on phorbol 12-myristate 13-acetate-stimulated AGS cells and human neutrophil elastase. The results indicate that the antiinflammatory activity associated with chamomile infusions from both the capitula and sifted flowers is most likely due to the inhibition of neutrophil elastase and gastric metalloproteinase-9 activity and secretion; the inhibition occurring in a concentration dependent manner. The promoter activity was inhibited as well and the decrease of metalloproteinase-9 expression was found to be associated with the inhibition of NF-kB driven transcription. The results further indicate that the flavonoid-7-glycosides, major constituents of chamomile flowers, may be responsible for the antiinflammatory action of the chamomile infusion observed here.
authors.affiliation: Dipartimento di Scienze Farmacologiche, Università degli Studi di Milano, Milan, Italy.
authors.forename: Michela
authors.initials: M
authors.lastname: Bulgari
authors.forename: Enrico
authors.initials: E
authors.lastname: Sangiovanni
authors.forename: Elisa
authors.initials: E
authors.lastname: Colombo
authors.forename: Omar
authors.initials: O
authors.lastname: Maschi
authors.forename: Donatella
authors.initials: D
authors.lastname: Caruso
authors.forename: Enrica
authors.initials: E
authors.lastname: Bosisio
authors.forename: Mario
authors.initials: M
authors.lastname: Dell'Agli
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D045744:Cell Line, Tumor; D020843:Chamomile / Q000737:chemistry*; D035264:Flowers / Q000737:chemistry; D006801:Humans; D019272:Leukocyte Elastase / Q000037:antagonists & inhibitors*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D061965:Matrix Metalloproteinase Inhibitors / Q000494:pharmacology*; D016328:NF-kappa B / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D011401:Promoter Regions, Genetic; D013274:Stomach Neoplasms / Q000201:enzymology; D014158:Transcription, Genetic
chemical_list: D000893:Anti-Inflammatory Agents; D061965:Matrix Metalloproteinase Inhibitors; D016328:NF-kappa B; D010936:Plant Extracts; D019272:Leukocyte Elastase; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9