title: An automatic wash method for dimethyl sulfoxide removal in autologous hematopoietic stem cell transplantation decreases the adverse effects related to infusion.
abstract: BACKGROUND
  Products cryopreserved with dimethyl sulfoxide (DMSO) in stem cell transplant (SCT) often cause many adverse effects during their infusion (major cardiovascular events, dyspnea … even death). These are especially frequent in pediatric patients. We tested if a fully automated and closed wash procedure (Sepax S-100, Biosafe) allowed us to maintain the absolute CD34+ cell number, cell viability, and engraftment potential, decreasing the untoward reactions.
  
  
  METHODS
  Forty-six washes of DMSO cryopreserved peripheral blood hematopoietic progenitor (HP) apheresis were studied. Blood aliquots were taken both after thawing and after washing to assess the total nucleated and CD34+ cell counts, as well as cell viability. The washed products were infused in 26 autologous SCTs (ASCTs). Results were compared with the 53 previous SCTs performed without DMSO removal.
  
  
  RESULTS
  After washing there were no significant differences between the pre- and postwashing CD34+ cell counts (p=0.08) or viability (p=0.68). No significant differences were observed between washed and nonwashed infusions in relation to the day of the neutrophil (p=0.46) and platelet (p=0.26) engraftment. One adverse event, abdominal pain, occurred during the washed cells infusions. When compared with the 14 untoward reactions that took place during the nonwashed HP infusions, significance was reached (p=0.00043).
  
  
  CONCLUSIONS
  The automatic method described is effective in terms of CD34+ cell recovery and viability in ASCT. Moreover, Sepax decreased significantly the untoward reactions during the infusion.
authors.affiliation: Servicio de Hematología del Hospital Virgen de la Arrixaca, and the Universidad de Murcia, Murcia, Spain. asanchezs2005@hotmail.com
authors.forename: Andrés
authors.initials: A
authors.lastname: Sánchez-Salinas
authors.forename: Valentín
authors.initials: V
authors.lastname: Cabañas-Perianes
authors.forename: Miguel
authors.initials: M
authors.lastname: Blanquer
authors.forename: María Juliana
authors.initials: MJ
authors.lastname: Majado
authors.forename: Carmen Luisa
authors.initials: CL
authors.lastname: Insausti
authors.forename: Jorge
authors.initials: J
authors.lastname: Monserrat
authors.forename: Maria Victoria
authors.initials: MV
authors.lastname: Sánchez-Ibáñez
authors.forename: Pilar
authors.initials: P
authors.lastname: Menchón
authors.forename: Ana
authors.initials: A
authors.lastname: García-Hernández
authors.forename: Joaquín
authors.initials: J
authors.lastname: Gómez-Espuch
authors.forename: Alfonso
authors.initials: A
authors.lastname: Morales
authors.forename: José M
authors.initials: JM
authors.lastname: Moraleda
mesh_terms: D000328:Adult; D000368:Aged; D001781:Blood Component Removal; D001793:Blood Preservation / Q000009:adverse effects* / Q000379:methods; D001804:Blood Transfusion, Autologous; D002470:Cell Survival / Q000187:drug effects; D002648:Child; D002675:Child, Preschool; D003451:Cryoprotective Agents / Q000009:adverse effects / Q000302:isolation & purification*; D004121:Dimethyl Sulfoxide / Q000009:adverse effects / Q000302:isolation & purification*; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects / Q000592:standards*; D006689:Hodgkin Disease / Q000628:therapy*; D006801:Humans; D008228:Lymphoma, Non-Hodgkin / Q000628:therapy; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000628:therapy
chemical_list: D003451:Cryoprotective Agents; D004121:Dimethyl Sulfoxide