title: The neurofibromin 1 type I isoform predominance characterises female population affected by sporadic breast cancer: preliminary data.
abstract: OBJECTIVE
  Neurofibromin 1 (NF1) as a tumour suppressor gene can give rise to several transcripts by an alternative splicing event, generated at least for CELF cofactors. At present, the NF1 isoforms and CELF splicing transcripts in sporadic breast cancer are unknown. The aim of the authors was to detect NF1 gene expression, the NF1 isoform ratio and the CELF transcripts present in sporadic breast cancer.
  
  
  METHODS
  Neurofibromin and RAS expression were analysed on tissue microarrays containing sporadic breast cancer (n=22), benign lesions (n=18, including six fibroadenomas, six fibrocystic changes and six ductal hyperplasias) and normal breast tissue (n=6) by immunohistochemistry assay. NF1 and CELF 3-6 RNA expression was performed by end point reverse transcription-PCR in the breast samples.
  
  
  RESULTS
  NF1 and RAS expression in breast tissues showed no differential expression by immunohistochemistry results. Interestingly, the authors observed a shift transition in the isoform transcripts, from type II in normal breast tissue to type I isoform in breast carcinomas. CELF cofactor expression failed to be related with the shift transition of NF1 in breast tissues.
  
  
  CONCLUSIONS
  These data suggest that there is a tendency for an NF1 expression shift transition from type II to type I isoform, which could comprise a significant event in the development and progression of sporadic breast cancer. This shift transition may not be related with CELF cofactors.
authors.affiliation: Laboratorio de Oncogenómica, Unidad de Investigación Médica en Enfermedades Oncológicas, Hospital de Oncología, CMN-SXXI, IMSS, Mexico, D.F.
authors.forename: Daniel
authors.initials: D
authors.lastname: Marrero
authors.forename: Raúl
authors.initials: R
authors.lastname: Peralta
authors.forename: Alejandra
authors.initials: A
authors.lastname: Valdivia
authors.forename: Antonio
authors.initials: A
authors.lastname: De la Mora
authors.forename: Pablo
authors.initials: P
authors.lastname: Romero
authors.forename: Miriam
authors.initials: M
authors.lastname: Parra
authors.forename: Nayeli
authors.initials: N
authors.lastname: Mendoza
authors.forename: Monica
authors.initials: M
authors.lastname: Mendoza
authors.forename: Dalila
authors.initials: D
authors.lastname: Rodriguez
authors.forename: Ernesto
authors.initials: E
authors.lastname: Camacho
authors.forename: Armando
authors.initials: A
authors.lastname: Duarte
authors.forename: German
authors.initials: G
authors.lastname: Castelazo
authors.forename: Enrique
authors.initials: E
authors.lastname: Vanegas
authors.forename: Israel
authors.initials: I
authors.lastname: Garcia
authors.forename: Claudia
authors.initials: C
authors.lastname: Vargas
authors.forename: Diego
authors.initials: D
authors.lastname: Arenas
authors.forename: Florinda
authors.initials: F
authors.lastname: Jimenez
authors.forename: Mauricio
authors.initials: M
authors.lastname: Salcedo
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D051743:CCAAT-Enhancer-Binding Protein-delta / Q000235:genetics* / Q000378:metabolism; D018270:Carcinoma, Ductal, Breast / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D018450:Disease Progression; D005260:Female; D018226:Fibroadenoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D005348:Fibrocystic Breast Disease / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D015870:Gene Expression; D020869:Gene Expression Profiling; D020022:Genetic Predisposition to Disease; D006801:Humans; D008875:Middle Aged; D025542:Neurofibromin 1 / Q000235:genetics* / Q000378:metabolism; D020033:Protein Isoforms; D046888:Tissue Array Analysis; D055815:Young Adult
chemical_list: C497252:CEBPD protein, human; D025542:Neurofibromin 1; D020033:Protein Isoforms; D051743:CCAAT-Enhancer-Binding Protein-delta