title: [Pharmacological mechanisms of ethyl acetate extraction of Tongbiheji for active T cell signaling molecules].
abstract: OBJECTIVE
  To confirm extracts of activity from Traditional Chinese Medicine TongBiHeJi,  study effect on two signaling pathways of T cells and clarify the pharmacological mechanisms of TongBiHeJi.
  
  
  METHODS
  Concanavalin(ConA) were added successively into rats lymphocytic culture with different extracts of activity from Traditional Chinese Medicine. After 24 hours,  CD71 expression rate on rat T lymphocytes activated with ConA was analyzed by flow cytometry. TCR,  CD28 and ICOS on T cells were detected after T lymphocytes of rat activated by ConA were cultivated with various EthylAcetate extraction of TongBiHeJi(TBHJ) and Methotrexate (MTX) for 48 hours.
  
  
  RESULTS
  CD71 expression rate on rat T lymphocytes induced by ConA was increased to 69.7%. TBHJ inhibited   the rate of CD3(+);CD71(+); expression(32.5%); ConA up-regulated TCR,  CD28 and ICOS expression on T cells obviously. There was different between ConA and positive control significantly(P<0.001). TBHJ could down-regulate obviously TCR, CD28 and ICOS expression on ConA-activated T lymphocytes with Concentration-dependent, especially ICOS. MTX inhibited CD3(+);CD71(+); and CD3(+);TCR(+); expression also.
  
  
  CONCLUSIONS
  TBHJ  inhibited T cells activation by adjusting two signaling pathways. That implied TBHJ could block CD28-ICOS signaling molecules to induce immunological tolerance. This study provided an experimental basis for application of TongBiHeJi to treatment of rheumatoid arthritis.
authors.affiliation: Department of JinKuiYaoLue Of Guangzhou Traditional Chinese Medicine University, Guangzhou, China.
authors.forename: Lin-kai
authors.initials: LK
authors.lastname: Guo
authors.forename: Guang-xing
authors.initials: GX
authors.lastname: Chen
authors.forename: Li-juan
authors.initials: LJ
authors.lastname: Liu
authors.forename: Lin-jun
authors.initials: LJ
authors.lastname: Peng
authors.forename: Xuan-jiang
authors.initials: XJ
authors.lastname: Tan
authors.forename: Qian-hua
authors.initials: QH
authors.lastname: Liao
authors.forename: Chuan
authors.initials: C
authors.lastname: Jiang
authors.forename: Ming-huan
authors.initials: MH
authors.lastname: Peng
mesh_terms: D000085:Acetates / Q000737:chemistry*; D000818:Animals; D001172:Arthritis, Rheumatoid / Q000188:drug therapy / Q000276:immunology; D018106:CD28 Antigens / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000494:pharmacology* / Q000627:therapeutic use; D060889:Inducible T-Cell Co-Stimulator Protein / Q000378:metabolism*; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism*
chemical_list: D000085:Acetates; D018106:CD28 Antigens; D004365:Drugs, Chinese Herbal; C556692:ICOS protein, human; D060889:Inducible T-Cell Co-Stimulator Protein; C007650:ethyl acetate