title: In vitro blockade of adhesion of breast cancer cells to endothelial cells using anti-inflammatory drugs.
abstract: BACKGROUND
  Increasing evidence suggests that a pro-inflammatory microenvironment affects distant metastasis of breast cancer cells, in particular by favoring tumor cell adhesion to endothelium. The aim of this study was to investigate the potential of different anti-inflammatory drugs to inhibit this effect in vitro.
  
  
  METHODS
  Breast cancer cells from the metastatic cell line KM22 were incubated with activated Human umbilical vein endothelial cells (HUVECs). Tumor cell adhesion was quantified by fluorescence microscopy. The anti-inflammatory drugs ibuprofen, aspirin (acetylsalicylic acid), diclofenac, and dexamethasone were used as inhibiting agents.
  
  
  RESULTS
  Aspirin and dexamethasone significantly reduced breast cancer cell adhesion to HUVECs (20.3%, p<0.000; and 25%, p<0.05, respectively). Ibuprofen and diclofenac did not significantly reduce tumor cell adhesion.
  
  
  CONCLUSIONS
  Aspirin and dexamethasone seem to be able to partly inhibit adhesion of breast cancer cells to endothelium. Future studies should attempt to optimize this effect in vitro, in preparation for potential in vivo trials.
authors.affiliation: Department of Gynecology and Obstetrics, University of Heidelberg Medical School, Voss-Strasse 9, D-69115 Heidelberg, Germany. Esther.Bischofs@med.uni-heidelberg.de
authors.forename: Esther
authors.initials: E
authors.lastname: Bischofs
authors.forename: Daniel
authors.initials: D
authors.lastname: Lubs
authors.forename: Friederike
authors.initials: F
authors.lastname: Fritzsche
authors.forename: Anne-Sophie
authors.initials: AS
authors.lastname: Meyer
authors.forename: Thomas
authors.initials: T
authors.lastname: Bruckner
authors.forename: Christof
authors.initials: C
authors.lastname: Sohn
authors.forename: Michael H R
authors.initials: MH
authors.lastname: Eichbaum
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000473:pathology*; D002448:Cell Adhesion / Q000187:drug effects*; D045744:Cell Line, Tumor; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000473:pathology; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008856:Microscopy, Fluorescence
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal