title: Long-term fate of silica nanoparticles interacting with human dermal fibroblasts.
abstract: The long-term fate of fluorescent non-porous FITC-SiO(2) nanoparticles of various sizes (10-200 nm) and charge is studied in the presence of human dermal fibroblasts. Particle aggregates are formed in the culture medium and uptaken, at least partially, by macropinocytosis. The smallest particles have a strong impact on cell viability and genotoxic effects can be observed for negatively-charged colloids 10 nm in size. Largest particles do not impact on cellular activity and can be monitored in cellulo via fluorescence and transmission electron microscopy studies over two weeks. These observations reveal a significant decrease in the size of silica particles located in endocytic vesicles. The dissolution process is confirmed by monitoring the cell culture medium that contains both colloidal and soluble silica species. Such dissolution can be explained on the sole basis of silica solubility and has great implication for the use of non-porous silica particles as intra-cellular drug release systems.
authors.affiliation: UPMC Univ Paris06, CNRS, Chimie de la Matière Condensée de Paris, Collège de France, F-75005 Paris, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Quignard
authors.forename: Gervaise
authors.initials: G
authors.lastname: Mosser
authors.forename: Michel
authors.initials: M
authors.lastname: Boissière
authors.forename: Thibaud
authors.initials: T
authors.lastname: Coradin
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D003470:Culture Media / Q000737:chemistry; D020405:Dermis / Q000166:cytology*; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects* / Q000648:ultrastructure; D016650:Fluorescein-5-isothiocyanate; D006801:Humans; D008027:Light; D008856:Microscopy, Fluorescence; D009153:Mutagens / Q000494:pharmacology; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D010873:Pinocytosis; D012542:Scattering, Radiation; D012822:Silicon Dioxide / Q000494:pharmacology*; D012996:Solutions; D013997:Time Factors
chemical_list: D003470:Culture Media; D009153:Mutagens; D012996:Solutions; D012822:Silicon Dioxide; D016650:Fluorescein-5-isothiocyanate