title: Apigenin inhibits cell migration through MAPK pathways in human bladder smooth muscle cells.
abstract: Apigenin, a nonmutagenic flavonoid, has been shown to possess free radical scavenging activities, anticarcinogenic properties, antioxidant and anti-inflammatory effects. Recently, apigenin was reported to cause gastric relaxation in murine. To assess possible effects of apigenin on migration of bladder smooth muscle (SM) cell, we isolated SM cells from peri-cancer tissue of human bladder and established a cell model that was capable to overexpress transiently MEKK1 (MEK kinase 1). Results showed that overexpression of active human MEKK1 by adenoviruses infection induced migration of human bladder smooth muscle (hBSM) cells and phosphorylation of MAPKs, ERK, JNK and p38, which are the downstream molecules of MEKK1. Then, hBSM cell overexpressing MEKK1 were exposed to apigenin (50 microM). Our data indicated that apigenin inhibited significantly activation/phosphorylation of MAPKs and migration of hBSM cells induced by MEKK1 overexpression. Besides, apigenin inhibited actin polymerization, which underlines muscle contraction and cell migration. The results suggest that apigenin inhibits activation of MAPKs and thereby the cell migration. The mechanism might be that apigenin blocks signal transmission from MEKK1 to MAPKs.
authors.affiliation: Department of Animal Biology, School of Husbandry and Veterinary Medicine, Jiangsu Polytechnic College of Agriculture and Forestry, Jurong, Jiangsu 212400, China.
authors.forename: Qingxin
authors.initials: Q
authors.lastname: Liu
authors.forename: Xianggui
authors.initials: X
authors.lastname: Chen
authors.forename: Guolin
authors.initials: G
authors.lastname: Yang
authors.forename: Xuewen
authors.initials: X
authors.lastname: Min
authors.forename: Maoxian
authors.initials: M
authors.lastname: Deng
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D047310:Apigenin / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects*; D002478:Cells, Cultured; D006801:Humans; D015151:Immunoblotting; D048728:MAP Kinase Kinase Kinase 1 / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D032389:Myocytes, Smooth Muscle / Q000187:drug effects* / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects; D001743:Urinary Bladder / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*
chemical_list: D047310:Apigenin; D020928:Mitogen-Activated Protein Kinases; D048728:MAP Kinase Kinase Kinase 1