title: Poly(ADP-ribose) in the skin and in melanomas.
abstract: Cutaneous melanoma (CM) and uveal melanoma (UM) represent the most aggressive pigment cell tumor types. Our investigation examined the signaling molecule poly(ADP-ribose) (PAR) in CM and UM. We have demonstrated PAR in keratinocytes, sebocytes, hair follicles, endothelial cells and in subcutaneous adipocytes in the normal skin indicating that PAR may regulate physiological functions in these cell types. Furthermore, CM cells were PAR positive and tumor invasion level/thickness of CM correlated with the PAR content of the cell nuclei, with higher Clark and Breslow indices and AJCC scores associating with higher PAR content. This correlation was especially marked in the samples of female patients. In UM tumors (n=12) a slight overall and strong perivascular PAR staining was observed with considerable individual variations. In view of recent successful clinical trials with PARP inhibitors as adjuvant chemotherapeutic agents, our results suggest that melanomas may display differential sensitivity towards this novel therapeutic modality which should be considered for the selection of patients.
authors.affiliation: Department of Medical Chemistry, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary.
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Géhl
authors.forename: Péter
authors.initials: P
authors.lastname: Bai
authors.forename: Edina
authors.initials: E
authors.lastname: Bodnár
authors.forename: Gabriella
authors.initials: G
authors.lastname: Emri
authors.forename: Éva
authors.initials: É
authors.lastname: Remenyik
authors.forename: János
authors.initials: J
authors.lastname: Németh
authors.forename: Pál
authors.initials: P
authors.lastname: Gergely
authors.forename: László
authors.initials: L
authors.lastname: Virág
authors.forename: Éva
authors.initials: É
authors.lastname: Szabó
mesh_terms: D017667:Adipocytes / Q000378:metabolism; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D042783:Endothelial Cells / Q000378:metabolism; D005260:Female; D018859:Hair Follicle / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000378:metabolism; D008297:Male; D008545:Melanoma / Q000378:metabolism* / Q000473:pathology; D008875:Middle Aged; D011064:Poly Adenosine Diphosphate Ribose / Q000378:metabolism*; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D015398:Signal Transduction; D012867:Skin / Q000166:cytology / Q000378:metabolism*; D012878:Skin Neoplasms / Q000378:metabolism* / Q000473:pathology; D014604:Uveal Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D011064:Poly Adenosine Diphosphate Ribose