title: Ameliorative effect of Grewia tenax (Forssk) fiori fruit extract on CCl(4)-induced oxidative stress and hepatotoxicity in rats.
abstract: The ethanol extract of Grewia tenax (GTE) fruit was tested for possible efficacy against carbon tetrachloride (CCl(4)) induced liver toxicity in Wistar albino rats. GTE at doses of 250 and 500 mg/kg were administered orally to CCl(4)-treated rats. Acute toxicity test and sleeping time determination were done with mice. The results showed that oral administration of GTE for 3 wk to rats significantly reduced the CCl(4)-induced elevated levels of serum glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, gamma-glutamyl transferase, alkaline phosphatase, bilirubin, cholesterol, high-density lipoproteins, low-density lipoproteins, very low density lipoproteins, and triglycerides. Moreover, it was found that the treatment with GTE significantly elevated the hemoglobin level in serum and increased the nonprotein sulfhydryl and total protein contents in the liver tissue, and a significant diminution was observed in the CCl(4)-induced elevated levels of malondialdehyde in the liver tissue. The biochemical findings were supported by an evaluation with liver histopathology. Pentobarbital-induced prolongation of narcolepsy in mice was shortened significantly by the extract. The observed hepatoprotective effect is believed to occur due to antioxidant properties of the contents of G. tenax extract, which may provide a new drug to be used for fighting liver diseases and it validates its folkloric use in anemic and other conditions.
authors.affiliation: Dept. of Pharmacognosy and Medicinal, Aromatic & Poisonous Plants Research Center (MAPPRC), College of Pharmacy, P.O. Box 2457, King Saud Univ., Riyadh 11451, Saudi Arabia.
authors.forename: Mansour S
authors.initials: MS
authors.lastname: Al-Said
authors.forename: Ramzi A
authors.initials: RA
authors.lastname: Mothana
authors.forename: Mohammed O
authors.initials: MO
authors.lastname: Al-Sohaibani
authors.forename: Syed
authors.initials: S
authors.lastname: Rafatullah
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001219:Aspartate Aminotransferases / Q000097:blood; D001663:Bilirubin / Q000097:blood; D002251:Carbon Tetrachloride / Q000633:toxicity*; D056486:Chemical and Drug Induced Liver Injury / Q000473:pathology*; D032382:Grewia / Q000737:chemistry*; D008075:Lipoproteins, HDL / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008315:Malondialdehyde / Q000032:analysis / Q000378:metabolism; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D023382:Toxicity Tests, Acute; D014280:Triglycerides / Q000097:blood; D005722:gamma-Glutamylcyclotransferase / Q000097:blood
chemical_list: D000975:Antioxidants; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D010936:Plant Extracts; D014280:Triglycerides; D008315:Malondialdehyde; D002251:Carbon Tetrachloride; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; D005722:gamma-Glutamylcyclotransferase; D001663:Bilirubin