title: Apical periodontium response to enamel matrix derivative as an intracanal medication in rat immature teeth with pulp necrosis: radiographic and histologic findings.
abstract: BACKGROUND
  The aim of this study was to evaluate the enamel matrix derivative (EMD) biomaterial in nonvital immature teeth.
  
  
  METHODS
  To arrest root development, pulpectomies were performed in the lower first molars of 36 4-week-old rats; the cavities were left exposed to the oral environment for 3 weeks. Then, chemical disinfection was performed, and triple antibiotic paste (TAP) or EMD was applied in the root canals. A control group did not receive any treatment. Radiographic and histological data were evaluated after 3 and 6 weeks.
  
  
  RESULTS
  At 3 weeks, TAP promoted a milder inflammatory response and increased root lengths compared with the control group. At 6 weeks, root development and reduced periapical lesions could be observed in both test groups, mainly because of the deposition of a cementum-like tissue. EMD promoted narrower canals compared with TAP (P < .05).
  
  
  CONCLUSIONS
  EMD deserves attention as a potential tool in the treatment of nonvital immature teeth. The ingrowth of cementum-like tissues into canal spaces favored dental wall thickness and may contribute to tooth resistance and support.
authors.affiliation: Pontificia Universidade Catolica do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
authors.forename: Roberta Kochenborger
authors.initials: RK
authors.lastname: Scarparo
authors.forename: Lenara
authors.initials: L
authors.lastname: Dondoni
authors.forename: Daiana Elisabeth
authors.initials: DE
authors.lastname: Böttcher
authors.forename: Fabiana Soares
authors.initials: FS
authors.lastname: Grecca
authors.forename: Jose A P
authors.initials: JA
authors.lastname: Figueiredo
authors.forename: Eraldo L
authors.initials: EL
authors.lastname: Batista
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000627:therapeutic use; D025021:Cementogenesis / Q000187:drug effects; D002939:Ciprofloxacin / Q000008:administration & dosage / Q000627:therapeutic use; D003739:Dental Cementum / Q000187:drug effects; D003746:Dental Enamel Proteins / Q000627:therapeutic use*; D003786:Dental Pulp Cavity / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000473:pathology; D003790:Dental Pulp Necrosis / Q000628:therapy*; D003809:Dentin, Secondary / Q000187:drug effects; D004338:Drug Combinations; D007091:Image Processing, Computer-Assisted; D008297:Male; D008795:Metronidazole / Q000008:administration & dosage / Q000627:therapeutic use; D008911:Minocycline / Q000008:administration & dosage / Q000627:therapeutic use; D009805:Odontogenesis / Q000187:drug effects; D010485:Periapical Periodontitis / Q000628:therapy*; D010486:Periapical Tissue / Q000187:drug effects; D010513:Periodontal Ligament / Q000187:drug effects; D019252:Radiography, Dental, Digital; D051381:Rats; D017208:Rats, Wistar; D012388:Root Canal Irrigants / Q000627:therapeutic use*; D013997:Time Factors; D019227:Tooth Apex / Q000187:drug effects*; D019553:Tooth, Nonvital / Q000628:therapy*
chemical_list: D000900:Anti-Bacterial Agents; D003746:Dental Enamel Proteins; D004338:Drug Combinations; D012388:Root Canal Irrigants; C109069:enamel matrix proteins; D008795:Metronidazole; D002939:Ciprofloxacin; D008911:Minocycline