title: Proteomic analysis of blood cells in fish exposed to chemotherapeutics: evidence for long term effects.
abstract: Proteomics technology are increasingly used in ecotoxicological studies to characterize and monitor biomarkers of exposure. The present study aims at identifying long term effects of malachite green (MG) exposure on the proteome of peripheral blood mononuclear cells (PBMC) from the Asian catfish, Pangasianodon hypophthalmus. A common (0.1 ppm) concentration for therapeutic treatment was applied twice with a 72 h interval. PBMC were collected directly at the end of the second bath of MG (T1) and after 1 month of decontamination (T2). Analytical 2D-DIGE gels were run and a total of 2551±364 spots were matched. Among them, MG induced significant changes in abundance of 116 spots with no recovery after one month of decontamination. Using LC-MS/MS and considering single identification per spot, we could identify 25 different proteins. Additionally, MG residues were measured in muscle and in blood indicating that leuco-MG has almost totally disappeared after one month of decontamination. This work highlights long term effects of MG treatment on the PBMC proteome from fish intended for human consumption.
authors.affiliation: Research Unit in Environmental and Evolutionary Biology (URBE), University of Namur (FUNDP), Rue de Bruxelles 61, B-5000, Namur, Belgium.
authors.forename: Marie-Aline
authors.initials: MA
authors.lastname: Pierrard
authors.forename: Patrick
authors.initials: P
authors.lastname: Kestemont
authors.forename: Nguyen Thanh
authors.initials: NT
authors.lastname: Phuong
authors.forename: Minh Phu
authors.initials: MP
authors.lastname: Tran
authors.forename: Edouard
authors.initials: E
authors.lastname: Delaive
authors.forename: Marie-Laëtitia
authors.initials: ML
authors.lastname: Thezenas
authors.forename: Marc
authors.initials: M
authors.lastname: Dieu
authors.forename: Martine
authors.initials: M
authors.lastname: Raes
authors.forename: Frédéric
authors.initials: F
authors.lastname: Silvestre
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology / Q000627:therapeutic use; D001773:Blood Cells / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D002397:Catfishes / Q000254:growth & development / Q000378:metabolism / Q000502:physiology; D004781:Environmental Exposure / Q000032:analysis; D005399:Fishes / Q000378:metabolism / Q000502:physiology; D005506:Food Contamination / Q000032:analysis; D005659:Fungicides, Industrial / Q000627:therapeutic use; D008954:Models, Biological; D020543:Proteome / Q000032:analysis* / Q000187:drug effects* / Q000378:metabolism; D040901:Proteomics; D012394:Rosaniline Dyes / Q000494:pharmacology* / Q000627:therapeutic use; D013997:Time Factors; D057825:Two-Dimensional Difference Gel Electrophoresis
chemical_list: D000890:Anti-Infective Agents; D005659:Fungicides, Industrial; D020543:Proteome; D012394:Rosaniline Dyes; C005095:malachite green; C015320:leucomalachite green