title: [Epigenetic aspects of early lymphogenic metastasis in breast cancer].
abstract: Metastasizing is one of the key stages in tumor development. Understanding this process is necessary for effective diagnosis, therapy and prediction of clinical outcome. Some recent data suggest the possibility of metastatic phenotype cells appearance at the early stages of tumor evolution in contravention with generally accepted hypothesis of linear metastatic process development. In this study we have performed a comparative analysis of the array-based DNA methylation profile in biopsy samples of patients with benign breast disorders, breast cancer and lymphogenous metastases. In some cases the biopsy samples dated back to different stages of the same tumor. For the analysis the GoldenGate Methylation Cancer Panel I was used. The DNA methylation level in 1,505 CpG-sites was similar in samples from patients with benign breast disorders and lymphogenous metastases. Our data support the hypothesis of the early appearance of cell clones responsible for the tumor limphogenous dissemination. Epigenetic component apparently plays an important role in this process.
authors.forename: N A
authors.initials: NA
authors.lastname: Skriabin
authors.forename: I N
authors.initials: IN
authors.lastname: Lebedev
authors.forename: E N
authors.initials: EN
authors.lastname: Tolmachëva
authors.forename: S V
authors.initials: SV
authors.lastname: Vtorushin
authors.forename: E M
authors.initials: EM
authors.lastname: Slonimskaia
authors.forename: N V
authors.initials: NV
authors.lastname: Cherbyntseva
mesh_terms: D000328:Adult; D000368:Aged; D001706:Biopsy; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology*; D018899:CpG Islands; D019175:DNA Methylation; D004273:DNA, Neoplasm / Q000378:metabolism; D044127:Epigenesis, Genetic; D005260:Female; D006801:Humans; D008198:Lymph Nodes / Q000473:pathology*; D008207:Lymphatic Metastasis; D046228:Microarray Analysis; D008875:Middle Aged
chemical_list: D004273:DNA, Neoplasm