title: Intrahepatic diacylglycerol content is associated with hepatic insulin resistance in obese subjects.
abstract: Data from studies in animal models indicate that certain lipid metabolites, particularly diacylglycerol, ceramide, and acylcarnitine, disrupt insulin action. We evaluated the relationship between the presence of these metabolites in the liver (assessed by mass spectrometry) and hepatic insulin sensitivity (assessed using a hyperinsulinemic-euglycemic clamp with stable isotope tracer infusion) in 16 obese adults (body mass index, 48 ± 9 kg/m²). There was a negative correlation between insulin-mediated suppression of hepatic glucose production and intrahepatic diacylglycerol (r = -0.609; P = .012), but not with intrahepatic ceramide or acylcarnitine. These data indicate that intrahepatic diacylglycerol is an important mediator of hepatic insulin resistance in obese people with nonalcoholic fatty liver disease.
authors.affiliation: Center for Human Nutrition, Washington University School of Medicine, St Louis, Missouri 63110, USA.
authors.forename: Faidon
authors.initials: F
authors.lastname: Magkos
authors.forename: Xiong
authors.initials: X
authors.lastname: Su
authors.forename: David
authors.initials: D
authors.lastname: Bradley
authors.forename: Elisa
authors.initials: E
authors.lastname: Fabbrini
authors.forename: Caterina
authors.initials: C
authors.lastname: Conte
authors.forename: J Christopher
authors.initials: JC
authors.lastname: Eagon
authors.forename: J Esteban
authors.initials: JE
authors.lastname: Varela
authors.forename: Elizabeth M
authors.initials: EM
authors.lastname: Brunt
authors.forename: Bruce W
authors.initials: BW
authors.lastname: Patterson
authors.forename: Samuel
authors.initials: S
authors.lastname: Klein
mesh_terms: D000328:Adult; D002331:Carnitine / Q000031:analogs & derivatives / Q000378:metabolism; D002518:Ceramides / Q000378:metabolism; D004075:Diglycerides / Q000378:metabolism*; D005260:Female; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007333:Insulin Resistance; D008099:Liver / Q000378:metabolism*; D008297:Male; D009767:Obesity, Morbid / Q000378:metabolism*
chemical_list: D002518:Ceramides; D004075:Diglycerides; C116917:acylcarnitine; D005947:Glucose; D002331:Carnitine