title: Adipose tissue can be generated in vitro by using adipocytes from human fat tissue mesenchymal stem cells seeded and cultured on fibrin gel sheet.
abstract: The current study has developed an innovative procedure to generate ex novo fat tissue by culturing adipocytes from human fat tissue mesenchymal stem cells (hFTMSCs) on fibrin gel sheet towards applications in medicine and cosmetology. Fibrin gel has been obtained by combining two components fibrinogen and thrombin collected by human peripheral blood. By this procedure it was possible to generate blocks of fibrin gel containing adipocytes within the gel that show similar features and consistency to human fat tissue mass. Results were assessed by histological staining methods, fluorescent immune-histochemistry staining as well photos by scanning electron microscopy (SEM) to demonstrate the adhesion and growth of cells in the fibrin gel. This result opens a real possibility for future clinical applications in the treatment of reconstructive and regenerative medicine where the use of stem cell may eventually be a unique solution or in the field of aesthetic medicine where autograft fat stem cells may grant for a safer and better outcome with long lasting results.
authors.affiliation: Department of Histo-pathology, Embryology, Genetics and Biotechnology for Tissue Transplants, Pham Ngoc, Thach Medical University, Ho Chi Minh City, Vietnam. toaiphd@yahoo.com
authors.forename: Cong Toai
authors.initials: CT
authors.lastname: Tran
authors.forename: Duy Thao
authors.initials: DT
authors.lastname: Huynh
authors.forename: Ciro
authors.initials: C
authors.lastname: Gargiulo
authors.forename: Le Bao Ha
authors.initials: le BH
authors.lastname: Tran
authors.forename: Minh Hang
authors.initials: MH
authors.lastname: Huynh
authors.forename: Khanh Hoa
authors.initials: KH
authors.lastname: Nguyen
authors.forename: Luis
authors.initials: L
authors.lastname: Filgueira
authors.forename: D Micheal
authors.initials: DM
authors.lastname: Strong
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D000273:Adipose Tissue / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000187:drug effects; D002469:Cell Separation; D002478:Cells, Cultured; D005337:Fibrin / Q000494:pharmacology*; D005434:Flow Cytometry; D005782:Gels / Q000494:pharmacology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010078:Oxazines / Q000378:metabolism; D013194:Staining and Labeling
chemical_list: D005782:Gels; D010078:Oxazines; D005337:Fibrin; C044808:nile red