title: IL-17 and IFN-γ expression in lymphocytes from patients with active tuberculosis correlates with the severity of the disease.
abstract: Th1 lymphocytes are crucial in the immune response against Mycobacterium tuberculosis. Nevertheless, IFN-γ alone is not sufficient in the complete eradication of the bacteria, suggesting that other cytokines might be required for pathogen removal. Th17 cells have been associated with M. tuberculosis infection, but the role of IL-17-producing cells in human TB remains to be understood. Therefore, we investigated the induction and regulation of IFN-γ and IL-17 during the active disease. TB patients were classified as High and Low Responder individuals according to their T cell responses against the antigen, and cytokine expression upon M. tuberculosis stimulation was investigated in peripheral blood and pleural fluid. Afterwards, the potential correlation among the proportions of cytokine-producing cells and clinical parameters was analyzed. In TB patients, M. tuberculosis induced IFN-γ and IL-17, but in comparison with BCG-vaccinated healthy donors, IFN-γ results were reduced significantly, and IL-17 was markedly augmented. Moreover, the main source of IL-17 was represented by CD4(+)IFN-γ(+)IL-17(+) lymphocytes, a Th1/Th17 subset regulated by IFN-γ. Interestingly, the ratio of antigen-expanded CD4(+)IFN-γ(+)IL-17(+) lymphocytes, in peripheral blood and pleural fluid from TB patients, was correlated directly with clinical parameters associated with disease severity. Indeed, the highest proportion of CD4(+)IFN-γ(+)IL-17(+) cells was detected in Low Responder TB patients, individuals displaying severe pulmonary lesions, and longest length of disease evolution. Taken together, the present findings suggest that analysis of the expansion of CD4(+)IFN-γ(+)IL-17(+) T lymphocytes in peripheral blood of TB patients might be used as an indicator of the clinical outcome in active TB.
authors.affiliation: Department of Biological Chemistry, University of Buenos Aires, School of Sciences, Buenos Aires, Argentina.
authors.forename: Javier O
authors.initials: JO
authors.lastname: Jurado
authors.forename: Virginia
authors.initials: V
authors.lastname: Pasquinelli
authors.forename: Ivana B
authors.initials: IB
authors.lastname: Alvarez
authors.forename: Delfina
authors.initials: D
authors.lastname: Peña
authors.forename: Ana I
authors.initials: AI
authors.lastname: Rovetta
authors.forename: Nancy L
authors.initials: NL
authors.lastname: Tateosian
authors.forename: Horacio E
authors.initials: HE
authors.lastname: Romeo
authors.forename: Rosa M
authors.initials: RM
authors.lastname: Musella
authors.forename: Domingo
authors.initials: D
authors.lastname: Palmero
authors.forename: H Eduardo
authors.initials: HE
authors.lastname: Chuluyán
authors.forename: Verónica E
authors.initials: VE
authors.lastname: García
mesh_terms: D000328:Adult; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000276:immunology; D020381:Interleukin-17 / Q000096:biosynthesis* / Q000276:immunology; D008297:Male; D009169:Mycobacterium tuberculosis; D012720:Severity of Illness Index; D018417:Th1 Cells / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D058504:Th17 Cells / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D014376:Tuberculosis / Q000097:blood* / Q000276:immunology / Q000473:pathology
chemical_list: D020381:Interleukin-17; D007371:Interferon-gamma