title: Synthesis of New 6-{[ω-(Dialkylamino(heterocyclyl)alkyl]thio}-3-R-2H-[1,2,4]triazino[2,3-c]quinazoline-2-ones and Evaluation of their Anticancer and Antimicrobial Activities.
abstract: Several novel 6-thio-3-R-2-oxo-2H-[1,2,4]triazino[2,3-c]quinazoline-based compounds containing an ω-(dialkylamino(heterocyclyl)]alkyl fragment were synthesized to examine their anticancer activity. Some of the 6-{[ω-(hetero-cyclyl)alkyl]thio}-3-R-2H-[1,2,4]triazino[2,3-c]quinazoline-2-ones (3.1-3.10) were obtained by the nucleophilic substitution of 6-[ω-halogenalkyl]thio-3-R-2H-[1,2,4]triazino[2,3-c]quinazoline-2-ones (2.1-2.8) with azaheterocycles. Alternatively, compounds 3.1-3.22 were synthesized by alkylation of 3-R-6-thio-2H-[1,2,4]triazino[2,3-c]quinazoline-2-ones potassium salts (1.1-1.4) with (2-chloroethyl)-N,N-dialkylamine hydrochlorides or 1-(2-chloroethyl)heterocycle hydrochlorides. The structures of compounds were elucidated by (1)H, (13)C NMR, LC-MS and EI-MS analysis. Then anticancer and antibacterial, bioluminescence inhibition of Photobacterium leiognathi Sh1 activities of the substances were tested in vitro. It was found that compound 3.18 possessed a wide range of anticancer activity against 27 cell lines of cancer: non-small cell lung, colon, CNS, ovarian, renal, prostate, breast, melanoma and leukemia (log GI(50) < -5.65). The "structure-activity" relationship was discussed. COMPARE analysis for synthesized anticancer active compounds was performed.
authors.affiliation: Department of Pharmacy, Zaporozhye State Medical University, Mayakovsky ave., 26, 69035, Zaporozhye, Ukraine.
authors.forename: Galina G
authors.initials: GG
authors.lastname: Berest
authors.forename: Olexiy Y
authors.initials: OY
authors.lastname: Voskoboynik
authors.forename: Sergiy I
authors.initials: SI
authors.lastname: Kovalenko
authors.forename: Inna S
authors.initials: IS
authors.lastname: Nosulenko
authors.forename: Lyudmyla M
authors.initials: LM
authors.lastname: Antypenko
authors.forename: Olexii M
authors.initials: OM
authors.lastname: Antypenko
authors.forename: Volodymyr M
authors.initials: VM
authors.lastname: Shvets
authors.forename: Andriy M
authors.initials: AM
authors.lastname: Katsev