title: Optimization of culture condition for ACEI and GABA production by lactic acid bacteria.
abstract: Gamma-aminobutyric acid (GABA) and angiotensin-converting enzyme inhibitor (ACEI) are compounds which can influence hypertension. The goal of this study is to optimize the culture condition for GABA and ACEI production by Lactobacillus plantarum NTU 102 fermented skim milk. In this study, we used 3-factor-3-level Box-Behnken design combining with response surface methodology, where the 3 factors represent the concentration of skim milk, the concentration of monosodium glutamate, and culture temperature. Best conditions for GABA and ACEI production differed. The results indicated that L. plantarum NTU 102 produced the highest combined levels of GABA and ACEI at 37 °C, in milk having 8% to 12% nonfat solids supplemented with 0.6% to 1% MSG. Agitation of the medium during fermentation had no effect on GABA or ACEI production but extended incubation (up to 6 d) increases levels of the bioactive compounds. L. plantarum NTU 102 fermented products may be a potential functional food source for regulating hypertension.
authors.affiliation: Dept. of Biochemical Science & Technology, College of Life Science, Natl. Taiwan Univ., Taipei, Taiwan.
authors.forename: Yi-Ting
authors.initials: YT
authors.lastname: Tung
authors.forename: Bao-Hong
authors.initials: BH
authors.lastname: Lee
authors.forename: Chin-Feng
authors.initials: CF
authors.lastname: Liu
authors.forename: Tzu-Ming
authors.initials: TM
authors.lastname: Pan
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000378:metabolism*; D000818:Animals; D015373:Bacterial Typing Techniques; D003470:Culture Media / Q000378:metabolism; D005285:Fermentation; D005516:Food Microbiology; D048191:Lactiplantibacillus plantarum / Q000254:growth & development* / Q000378:metabolism; D008892:Milk; D012970:Sodium Glutamate / Q000378:metabolism; D013696:Temperature; D005680:gamma-Aminobutyric Acid / Q000096:biosynthesis*
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D003470:Culture Media; D005680:gamma-Aminobutyric Acid; D012970:Sodium Glutamate