title: Simple DNA transformation in Pseudomonas based on the Yoshida effect.
abstract: Current protocols of recombinant DNA research, including gene cloning and complementation, quantification of gene expression and tagging with reporter proteins, are usually limited by the availability of effective bacteria transformation tools different from Escherichia coli. This is particularly relevant with respect to the Pseudomonas species due to their biotechnological and sanitary importance. Here, we describe an optimized and efficient plasmid transference protocol based on the Yoshida effect, a method that relies on DNA uptake mediated by friction forces. The main advantages of this method are: (i) no competent cell preparation is needed, (ii) cells in any physiological state can be used, (iii) the procedure is performed directly on agar plates and (iv) the protocol, which is neither time-consuming nor labor-intensive, offers good efficiency. This approach promises to become the gold standard for day to day genetic manipulation in Pseudomonas.
authors.affiliation: Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas, Madrid, Spain.
authors.forename: Jerónimo
authors.initials: J
authors.lastname: Rodríguez-Beltrán
authors.forename: Hamouda
authors.initials: H
authors.lastname: Elabed
authors.forename: Kamel
authors.initials: K
authors.lastname: Gaddour
authors.forename: Jesús
authors.initials: J
authors.lastname: Blázquez
authors.forename: Alexandro
authors.initials: A
authors.lastname: Rodríguez-Rojas
mesh_terms: D001419:Bacteria; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli; D018014:Gene Transfer Techniques; D010957:Plasmids; D011549:Pseudomonas / Q000235:genetics*; D014169:Transformation, Bacterial
chemical_list: D004269:DNA, Bacterial