title: Simultaneous extraction and separation of flavonols and flavones from Chamaecyparis obtusa by multi-phase extraction using an ionic liquid-modified microsphere polymer.
abstract: BACKGROUND
  Flavonols and flavones, especially quercitrin, myricetin and amentoflavone, are the main anti-bacterial and anti-cancer compounds in Chamaecyparis obtuse. Multi-phase extraction is a new method that can extract and separate target compounds simultaneously.
  
  
  OBJECTIVE
  An amino ionic liquid immobilised microsphere polymer was used as a multi-phase extraction sorbent to extract and separate quercitrin, myricetin and amentoflavone from Chamaecyparis obtusa.
  
  
  METHODS
  The sorbent and Chamaecyparis obtusa powder were packed into a single cartridge. Using a fixed volume of methanol with five repetitions, the target compounds were extracted from the powder to the sorbent. The sorbent was then washed with n-hexane to remove any interfering species and the target compounds were eluted sequentially using water, methanol and methanol containing 1% acetic acid (vol.).
  
  
  RESULTS
  Under the optimised conditions, 0.45 mg/g of quercitrin, 0.18 mg/g of myricetin and 0.12 mg/g of amentoflavone from 2.0 g of powder were obtained by multi-phase extraction using 0.3 g of sorbent.
  
  
  CONCLUSIONS
  The method described has a low deviation error, requires a small amount of solvent and is highly selective and reproducible.
authors.affiliation: Department of Chemical Engineering, Inha University, 253 Yonghyun-Dong, Nam-Ku, Incheon, 402-751, Korea.
authors.forename: Minglei
authors.initials: M
authors.lastname: Tian
authors.forename: Wentao
authors.initials: W
authors.lastname: Bi
authors.forename: Kyung Ho
authors.initials: KH
authors.lastname: Row
mesh_terms: D000327:Adsorption; D044946:Biflavonoids / Q000302:isolation & purification; D029780:Chamaecyparis / Q000737:chemistry*; D005591:Chemical Fractionation / Q000295:instrumentation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D047309:Flavones / Q000737:chemistry / Q000302:isolation & purification*; D005419:Flavonoids / Q000302:isolation & purification; D044948:Flavonols / Q000737:chemistry / Q000302:isolation & purification*; D052578:Ionic Liquids; D008863:Microspheres; D011794:Quercetin / Q000031:analogs & derivatives / Q000302:isolation & purification; D059027:Recycling; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry
chemical_list: D044946:Biflavonoids; D047309:Flavones; D005419:Flavonoids; D044948:Flavonols; D052578:Ionic Liquids; D012997:Solvents; C012526:quercitrin; C040015:myricetin; C011164:amentoflavone; D011794:Quercetin