title: Characterization of superparamagnetic iron oxide nanoparticles and its application in protein purification.
abstract: The application of surface modified magnetic adsorbent particles in combination with magnetic separation techniques has received considerable awareness in recent years. There is a particular need in protein purification and analysis for specific, functional and generic methods of protein binding on solid supports. Nanoscale superparamagnetic iron oxide particles have been used to purify a natural coagulant protein extracted from Moringa oleifera seeds. Spectrophotometric analysis of the coagulant protein was performed using synthetic clay solution as substrate. Protein binding with carboxyl and silica surface modified superparamagnetic iron oxide nanoparticles (SPION) were compared with the known carboxyl methyl cellulose (CMC) beads of approximately 1 microm. SPION modified with carboxyl surface showed higher binding capacity towards the coagulant protein compared to the CMC beads. The high surface area to volume ratio of the carboxyl-coated SPION resulted in high binding capacity and rapid adsorption kinetics of the crude protein extract. The purification and molecular weight of coagulant protein is analyzed by SDS-PAGE. This approach utilizes the most efficient, feasible and economical method of coagulant protein purification and it can also be applicable to other proteins that possess similar properties.
authors.affiliation: Division of Environmental Microbiology, Royal Institute of Technology, Stockholm, 106 91 Stockholm, Sweden.
authors.forename: Chuka
authors.initials: C
authors.lastname: Okoli
authors.forename: Andrea
authors.initials: A
authors.lastname: Fornara
authors.forename: Jian
authors.initials: J
authors.lastname: Qin
authors.forename: Muhammet S
authors.initials: MS
authors.lastname: Toprak
authors.forename: Gunnel
authors.initials: G
authors.lastname: Dalhammar
authors.forename: Mamoun
authors.initials: M
authors.lastname: Muhammed
authors.forename: Gunaratna K
authors.initials: GK
authors.lastname: Rajarao
mesh_terms: D000327:Adsorption; D003029:Coagulants / Q000302:isolation & purification; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008970:Molecular Weight; D029588:Moringa oleifera / Q000737:chemistry; D010940:Plant Proteins / Q000302:isolation & purification*; D013499:Surface Properties
chemical_list: D003029:Coagulants; D058185:Magnetite Nanoparticles; D010940:Plant Proteins