title: Separation and identification of antibacterial chamomile components using OPLC, bioautography and GC-MS.
abstract: Components of 50% aqueous ethanol chamomile (Matricaria recutica L.) flower extract, previously found antibacterial in a TLC-bioautographic study, were separated and isolated by the use of on-line overpressured layer chromatography (OPLC). This system consisted of an OPLC 50 BS system, an on-line coupled flow-through UV detector, and a manual fraction collector. The collected fractions were investigated by GC-MS analysis and by TLC re-chromatography with subsequent visualization, performed after use of the vanillin-sulphuric acid reagent, or under UV illumination, or applying bioautographic detection. The main compounds of the collected 11 fractions were identified by GC-MS. The results showed that the antibacterial effect of 50% aqueous ethanol extract of chamomile is ascribable to cis-, trans-spiroethers, and the coumarins like herniarin and umbelliferone.
authors.affiliation: Plant Protection Institute, Hungarian Academy of Sciences, Budapest, Hungary. moricz_am@nki.hu
authors.forename: Agnes M
authors.initials: AM
authors.lastname: Móricz
authors.forename: Szabolcs
authors.initials: S
authors.lastname: Szarka
authors.forename: Péter G
authors.initials: PG
authors.lastname: Ott
authors.forename: Eva B
authors.initials: EB
authors.lastname: Héthelyi
authors.forename: Eva
authors.initials: E
authors.lastname: Szoke
authors.forename: Erno
authors.initials: E
authors.lastname: Tyihák
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000302:isolation & purification / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects; D001681:Biological Assay; D002855:Chromatography, Thin Layer; D008401:Gas Chromatography-Mass Spectrometry; D031168:Matricaria / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D018514:Plant Structures / Q000737:chemistry*; D011549:Pseudomonas / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents