title: Antioxidant activity of mulberry fruit extracts.
abstract: Phenolic compounds were extracted from the fruits of Morus nigra and Morus alba using methanol and acetone. The sugar-free extracts (SFEs) were prepared using Amberlite XAD-16 column chromatography. All of the SFEs exhibited antioxidant potential as determined by ABTS (0.75-1.25 mmol Trolox/g), DPPH (2,2-diphenyl-1-picrylhydrazyl) (EC(50) from 48 μg/mL to 79 μg/mL), and reducing power assays. However, a stronger activity was noted for the SFEs obtained from Morus nigra fruits. These extracts also possessed the highest contents of total phenolics: 164 mg/g (methanolic SFE) and 173 mg/g (acetonic SFE). The presence of phenolic acids and flavonoids in the extracts was confirmed using HPLC method and chlorogenic acid and rutin were found as the dominant phenolic constituents in the SFEs.
authors.affiliation: Department of Chemistry, Palosa Campus, Abdul Wali Khan University, 24420 Charsadda, KPK, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Arfan
authors.affiliation: Department of Chemistry, Palosa Campus, Abdul Wali Khan University, 24420 Charsadda, KPK, Pakistan.
authors.forename: Rasool
authors.initials: R
authors.lastname: Khan
authors.affiliation: Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Tuwima Street 10, 10-747 Olsztyn, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Rybarczyk
authors.affiliation: Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Tuwima Street 10, 10-747 Olsztyn, Poland.
authors.forename: Ryszard
authors.identifier: 0000-0001-9731-0045
authors.initials: R
authors.lastname: Amarowicz
mesh_terms: D000096:Acetone / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D005638:Fruit / Q000737:chemistry*; D062385:Hydroxybenzoates / Q000494:pharmacology; D000432:Methanol / Q000494:pharmacology; D031625:Morus / Q000737:chemistry*; D010084:Oxidation-Reduction / Q000187:drug effects; D010636:Phenols / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000975:Antioxidants; D062385:Hydroxybenzoates; D010636:Phenols; D010936:Plant Extracts; D000096:Acetone; C017616:phenolic acid; D000432:Methanol