title: The sub-cellular localization of Sulfolobus DNA replication.
abstract: Analyses of the DNA replication-associated proteins of hyperthermophilic archaea have yielded considerable insight into the structure and biochemical function of these evolutionarily conserved factors. However, little is known about the regulation and progression of DNA replication in the context of archaeal cells. In the current work, we describe the generation of strains of Sulfolobus solfataricus and Sulfolobus acidocaldarius that allow the incorporation of nucleoside analogues during DNA replication. We employ this technology, in conjunction with immunolocalization analyses of replisomes, to investigate the sub-cellular localization of nascent DNA and replisomes. Our data reveal a peripheral localization of replisomes in the cell. Furthermore, while the two replication forks emerging from any one of the three replication origins in the Sulfolobus chromosome remain in close proximity, the three origin loci are separated.
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK.
authors.forename: Tamzin
authors.initials: T
authors.lastname: Gristwood
authors.forename: Iain G
authors.initials: IG
authors.lastname: Duggin
authors.forename: Michaela
authors.initials: M
authors.lastname: Wagner
authors.forename: Sonja V
authors.initials: SV
authors.lastname: Albers
authors.forename: Stephen D
authors.initials: SD
authors.lastname: Bell
mesh_terms: D001973:Bromodeoxyuridine / Q000032:analysis; D002453:Cell Cycle; D004261:DNA Replication; D019641:DNA, Archaeal / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry; D004259:DNA-Directed DNA Polymerase / Q000032:analysis; D003857:Deoxyuridine / Q000031:analogs & derivatives / Q000032:analysis; D009097:Multienzyme Complexes / Q000032:analysis; D016945:Sulfolobus acidocaldarius / Q000235:genetics* / Q000378:metabolism; D048229:Sulfolobus solfataricus / Q000235:genetics* / Q000378:metabolism; D000099272:Replisomes
chemical_list: D001973:Bromodeoxyuridine; D019641:DNA, Archaeal; D004259:DNA-Directed DNA Polymerase; D003857:Deoxyuridine; D009097:Multienzyme Complexes; D000099272:Replisomes; C031086:5-ethynyl-2'-deoxyuridine