title: Serum biochemical indicators of hepatobiliary function in dogs following prolonged anaesthesia with sevoflurane or isoflurane.
abstract: OBJECTIVE
  To investigate the changes in serum enzymes considered as biochemical indicators of hepatobiliary function in dogs following 5 hours of anaesthesia with isoflurane (ISO) or sevoflurane (SEVO).
  
  
  METHODS
  Experimental randomized crossover study, with intervals of at least 15 days between successive treatments.
  
  
  METHODS
  Eight healthy adult mongrel dogs, four male, four female, weight 13.6-21.6 kg.
  
  
  METHODS
  Treatments consisted of anaesthesia with ISO or SEVO at 1 or 1.5 minimum alveolar concentration (MAC) delivered in oxygen. MAC was taken as 1.39% for ISO and 2.36% for SEVO. Anaesthesia was induced by mask then, after endotracheal intubation, maintained according to the treatment protocol using a small animal circle system. Cardiopulmonary monitoring was carried out. Venous blood samples, obtained by needle puncture, were taken at 24 hours and 2, 7 and 14 days post anaesthesia. Serum concentrations of total protein, aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase, (LDH), alkaline phosphatase (ALP), gamma-glutamyltransferese and total bilirubin were measured. Changes with time and with treatment were compared by Friedman analysis, Wilcoxon Signed test and Kruskal-Wallis test as relevant. p- value < 0.05 was considered significant.
  
  
  RESULTS
  Compared to base-line values, at 24 hours post-anaesthesia there were significant increases in AST, ALT, ALP and LDH following one or more of the treatments, but by 2 days residual changes were not significant. At 24 hours, AST for treatment 1.5 MAC ISO was higher than 1 MAC ISO (p < 0.002), and LDH higher for 1.5 MAC SEVO than 1 MAC SEVO.
  
  
  CONCLUSIONS
  Both ISO and SEVO, at concentrations used for clinical anaesthesia, produce transient moderate effects on some hepatobiliary enzyme concentrations in dogs.
authors.affiliation: Department of Clinical Science, College of Veterinary Medicine, China Agricultural University, Beijing, PR China.
authors.forename: ZhanKui
authors.initials: Z
authors.lastname: Yuan
authors.forename: JieXin
authors.initials: J
authors.lastname: Liu
authors.forename: XiuTing
authors.initials: X
authors.lastname: Liang
authors.forename: DeGui
authors.initials: D
authors.lastname: Lin
mesh_terms: D000410:Alanine Transaminase / Q000097:blood / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000097:blood / Q000378:metabolism; D018685:Anesthetics, Inhalation / Q000009:adverse effects / Q000494:pharmacology*; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood / Q000378:metabolism; D001663:Bilirubin / Q000097:blood / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000097:blood / Q000662:veterinary*; D004283:Dog Diseases / Q000097:blood / Q000139:chemically induced*; D004285:Dogs; D005260:Female; D006339:Heart Rate / Q000187:drug effects; D007530:Isoflurane / Q000009:adverse effects / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000097:blood / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000502:physiology; D008297:Male; D008738:Methyl Ethers / Q000009:adverse effects / Q000494:pharmacology*; D000077149:Sevoflurane; D005723:gamma-Glutamyltransferase / Q000097:blood / Q000378:metabolism
chemical_list: D018685:Anesthetics, Inhalation; D008738:Methyl Ethers; D000077149:Sevoflurane; D007530:Isoflurane; D007770:L-Lactate Dehydrogenase; D005723:gamma-Glutamyltransferase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; D001663:Bilirubin