title: Selective and nonselective neuronal NOS inhibitors impair cognitive function in the three panel runway and passive avoidance tasks in rats.
abstract: The aim of this study was to compare the effects of both neuronal NOS (nNOS) and inducible NOS (iNOS) inhibitor 2-iminobiotin, with the more selective nNOS inhibitor N(ω)-propyl-L-arginine (NPLA) and selective inducible NOS (iNOS) inhibitor aminoguanidine, on emotional learning, working memory and reference memory, by using three panel runway and passive avoidance paradigm in order to clarify the role of distinct isoforms of NOS in the regulation of learning and memory functions. NPLA and 2-iminobiotin significantly increased the number of errors and latency of working and reference memory performances of rats in three panel runway paradigm and impairs retention for the passive avoidance task. However, aminoguanidine did not affect cognitive functions in three panel runway and passive avoidance test. The effect of NPLA and 2-iminobiotin was reversed by pretreatment with a NOS substrate L-arginine. In conclusion, NPLA and 2-iminobiotin impaired cognitive functions in different kind of tasks used in this study and the effect of NPLA and 2-iminobiotin was found to be NO-dependent. Our results may confirm that nNOS plays the key role on emotional learning, working memory and reference memory in rats.
authors.affiliation: Department of Pharmacology and Experimental Medical Research and Application Center, Faculty of Medicine, Kocaeli University, Umuttepe, 41380 Kocaeli, Turkey. tijenutkan@hotmail.com
authors.forename: Tijen
authors.initials: T
authors.lastname: Utkan
authors.forename: Semil Selcen
authors.initials: SS
authors.lastname: Gocmez
authors.forename: Cuneyt
authors.initials: C
authors.lastname: Ozer
authors.forename: Nejat
authors.initials: N
authors.lastname: Gacar
authors.forename: Feyza
authors.initials: F
authors.lastname: Aricioglu
mesh_terms: D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D001362:Avoidance Learning / Q000187:drug effects / Q000502:physiology; D001710:Biotin / Q000031:analogs & derivatives / Q000494:pharmacology; D003071:Cognition / Q000187:drug effects* / Q000502:physiology; D004644:Emotions / Q000187:drug effects / Q000502:physiology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006146:Guanidines / Q000494:pharmacology; D008297:Male; D008570:Memory, Short-Term / Q000187:drug effects / Q000502:physiology; D023421:Models, Animal; D009569:Nitric Oxide / Q000502:physiology; D052248:Nitric Oxide Synthase Type I / Q000037:antagonists & inhibitors* / Q000502:physiology; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004791:Enzyme Inhibitors; D006146:Guanidines; C109599:N(omega)-propylarginine; D009569:Nitric Oxide; D001710:Biotin; D001120:Arginine; D052248:Nitric Oxide Synthase Type I; D052247:Nitric Oxide Synthase Type II; C496322:Nos1 protein, rat; C496318:Nos2 protein, rat; C028072:2-iminobiotin; C004479:pimagedine