title: Axonal transport of adeno-associated viral vectors is serotype-dependent.
abstract: We have previously shown that adeno-associated virus type 2 (AAV2) undergoes anterograde axonal transport in rat and non-human primate brain. We screened other AAV serotypes for axonal transport and found that AAV6 is transported almost exclusively in a retrograde direction and, in the same way as AAV2, it is also neuron-specific in rat brain. Our findings show that axonal transport of AAV is serotype dependent and this has implications for gene therapy of neurological diseases such as Huntington's disease.
authors.affiliation: Department of Neurosurgery, University of California San Francisco, San Francisco, CA 94103-0555, USA.
authors.forename: E A
authors.initials: EA
authors.lastname: Salegio
authors.forename: L
authors.initials: L
authors.lastname: Samaranch
authors.forename: A P
authors.initials: AP
authors.lastname: Kells
authors.forename: G
authors.initials: G
authors.lastname: Mittermeyer
authors.forename: W
authors.initials: W
authors.lastname: San Sebastian
authors.forename: S
authors.initials: S
authors.lastname: Zhou
authors.forename: J
authors.initials: J
authors.lastname: Beyer
authors.forename: J
authors.initials: J
authors.lastname: Forsayeth
authors.forename: K S
authors.initials: KS
authors.lastname: Bankiewicz
mesh_terms: D000818:Animals; D001370:Axonal Transport; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D003342:Corpus Striatum / Q000166:cytology / Q000378:metabolism; D000229:Dependovirus / Q000145:classification / Q000235:genetics*; D005455:Fluorescent Antibody Technique; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D006816:Huntington Disease / Q000235:genetics / Q000628:therapy; D019636:Neurodegenerative Diseases / Q000235:genetics / Q000628:therapy; D051381:Rats; D017207:Rats, Sprague-Dawley; D012703:Serotyping; D013045:Species Specificity; D013788:Thalamus / Q000166:cytology / Q000378:metabolism; D014161:Transduction, Genetic / Q000379:methods*
chemical_list: D049452:Green Fluorescent Proteins