title: UGT87A2, an Arabidopsis glycosyltransferase, regulates flowering time via FLOWERING LOCUS C.
abstract: • Family 1 glycosyltransferases comprise the greatest number of glycosyltransferases found in plants. The widespread occurrence and diversity of glycosides throughout the plant kingdom underscore the importance of these glycosyltransferases. • Here, we describe the identification and characterization of a late-flowering Arabidopsis (Arabidopsis thaliana) mutant, in which a putative family 1 glycosyltransferase gene, UGT87A2, was disrupted. The role and possible mechanism of UGT87A2 in the regulation of flowering were analyzed by molecular, genetic and cellular approaches. • The ugt87a2 mutant exhibited late flowering in both long and short days, and its flowering was promoted by vernalization and gibberellin. Furthermore, the mutant flowering phenotype was rescued by the wild-type UGT87A2 gene in complementation lines. Interestingly, the expression of the flowering repressor FLOWERING LOCUS C was increased substantially in the mutant, but decreased to the wild-type level in complementation lines, with corresponding changes in the expression levels of the floral integrators and floral meristem identity genes. The expression of UGT87A2 was developmentally regulated and its protein products were distributed in both cytoplasm and nucleus. • Our findings imply that UGT87A2 regulates flowering time via the flowering repressor FLOWERING LOCUS C. These data highlight an important role for the family 1 glycosyltransferases in the regulation of plant flower development.
authors.affiliation: The Key Lab of Plant Cell Engineering and Germplasm Innovation, Education Ministry of China, School of Life Science, Shandong University, No. 27 Shanda South Road, Jinan, Shandong 250100, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.affiliation: The Key Lab of Plant Cell Engineering and Germplasm Innovation, Education Ministry of China, School of Life Science, Shandong University, No. 27 Shanda South Road, Jinan, Shandong 250100, China.
authors.forename: Shang-Hui
authors.initials: SH
authors.lastname: Jin
authors.affiliation: The Key Lab of Plant Cell Engineering and Germplasm Innovation, Education Ministry of China, School of Life Science, Shandong University, No. 27 Shanda South Road, Jinan, Shandong 250100, China.
authors.forename: Hong-Qun
authors.initials: HQ
authors.lastname: Hu
authors.affiliation: The Key Lab of Plant Cell Engineering and Germplasm Innovation, Education Ministry of China, School of Life Science, Shandong University, No. 27 Shanda South Road, Jinan, Shandong 250100, China.
authors.forename: Yan-Guo
authors.initials: YG
authors.lastname: Sun
authors.affiliation: The Key Lab of Plant Cell Engineering and Germplasm Innovation, Education Ministry of China, School of Life Science, Shandong University, No. 27 Shanda South Road, Jinan, Shandong 250100, China.
authors.forename: Yan-Wen
authors.initials: YW
authors.lastname: Wang
authors.affiliation: The Key Lab of Plant Cell Engineering and Germplasm Innovation, Education Ministry of China, School of Life Science, Shandong University, No. 27 Shanda South Road, Jinan, Shandong 250100, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Han
authors.affiliation: The Key Lab of Plant Cell Engineering and Germplasm Innovation, Education Ministry of China, School of Life Science, Shandong University, No. 27 Shanda South Road, Jinan, Shandong 250100, China.
authors.forename: Bing-Kai
authors.initials: BK
authors.lastname: Hou
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000166:cytology / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D035264:Flowers / Q000166:cytology / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D005816:Genetic Complementation Test; D005838:Genotype; D016695:Glycosyltransferases / Q000235:genetics* / Q000378:metabolism; D026161:MADS Domain Proteins / Q000235:genetics*; D018519:Meristem / Q000166:cytology / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D008954:Models, Biological; D008969:Molecular Sequence Data; D009154:Mutation; D009928:Organ Specificity; D010641:Phenotype; D017440:Photoperiod; D018515:Plant Leaves / Q000166:cytology / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D011401:Promoter Regions, Genetic / Q000235:genetics; D013997:Time Factors; D015854:Up-Regulation
chemical_list: D029681:Arabidopsis Proteins; C418902:At5g10140 protein, Arabidopsis; D026161:MADS Domain Proteins; C000590312:UGT87A2 protein, Arabidopsis; D016695:Glycosyltransferases