title: Preparation, evaluation and characterization of quercetin-molecularly imprinted polymer for preconcentration and clean-up of catechins.
abstract: Molecularly imprinted polymer (MIP) for solid extraction and preconcentration of catechins have been successfully prepared by a thermal polymerization method using quercetin as template, 4-vinylpyridine as functional monomer and ethylene glycol dimethacrylate as crosslinker. A solution mixture of acetone and acetonitrile was used as porogen. Systematic investigations of the influence of monomer, cross-linker, porogen, as well as polymerization conditions on the properties of the MIPs were carried out. The quercetin MIPs were evaluated according to their selective recognition properties for quercetin, structurally related compounds (catechin, epigallocatechin gallate and epicatechin) and a unrelated compound of similar molecular size (α-tocopherol). Good binding was observed for quercetin, catechin and epigallocatechin gallate with an optimized MIP in a solid phase extraction system. Adsorption and kinetic characteristics were evaluated for catechins which indicated that the synthesized polymer had high adsorption capacity and contained homogeneous binding sites. Chemical and morphological characterization of the MIP was investigated by FTIR, SEM and BET, which confirmed a high degree of polymerization. Finally, the MIP was successfully applied to the clean-up and preconcentration of catechins from several natural samples.
authors.affiliation: Grupo de Polímeros, Centro de Investigacións Tecnológicas, Universidade de A Coruña, Ferrol, Spain.
authors.forename: María del Mar Castro
authors.initials: Mdel M
authors.lastname: López
authors.forename: M C Cela
authors.initials: MC
authors.lastname: Pérez
authors.forename: María Sonia Dopico
authors.initials: MS
authors.lastname: García
authors.forename: José Manuel López
authors.initials: JM
authors.lastname: Vilariño
authors.forename: María Victoria González
authors.initials: MV
authors.lastname: Rodríguez
authors.forename: Luis F Barral
authors.initials: LF
authors.lastname: Losada
mesh_terms: D000327:Adsorption; D002392:Catechin / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D006860:Hydrogen Bonding; D007700:Kinetics; D054802:Molecular Imprinting; D011108:Polymers / Q000737:chemistry*; D011794:Quercetin / Q000737:chemistry*; D052616:Solid Phase Extraction
chemical_list: D011108:Polymers; D002392:Catechin; D011794:Quercetin; C045651:epigallocatechin gallate