title: Identification and characterization of the RLIP/RALBP1 interacting protein Xreps1 in Xenopus laevis early development.
abstract: BACKGROUND
  The FGF/Ras/Ral/RLIP pathway is required for the gastrulation process during the early development of vertebrates. The Ral Interacting Protein (RLIP also known as RalBP1) interacts with GTP-bound Ral proteins. RLIP/RalBP1 is a modular protein capable of participating in many cellular functions.
  
  
  RESULTS
  To investigate the role of RLIP in early development, a two-hybrid screening using a library of maternal cDNAs of the amphibian Xenopus laevis was performed. Xreps1 was isolated as a partner of RLIP/RalBP1 and its function was studied. The mutual interacting domains of Xreps1 and Xenopus RLIP (XRLIP) were identified. Xreps1 expressed in vivo, or synthesized in vitro, interacts with in vitro expressed XRLIP. Interestingly, targeting of Xreps1 or the Xreps1-binding domain of XRLIP (XRLIP(469-636)) to the plasma membrane through their fusion to the CAAX sequence induces a hyperpigmentation phenotype of the embryo. This hyperpigmented phenotype induced by XRLIP(469-636)-CAAX can be rescued by co-expression of a deletion mutant of Xreps1 restricted to the RLIP-binding domain (Xreps1(RLIP-BD)) but not by co-expression of a cDNA coding for a longer form of Xreps1.
  
  
  CONCLUSIONS
  We demonstrate here that RLIP/RalBP1, an effector of Ral involved in receptor-mediated endocytosis and in the regulation of actin dynamics during embryonic development, also interacts with Reps1. Although these two proteins are present early during embryonic development, they are active only at the end of gastrulation. Our results suggest that the interaction between RLIP and Reps1 is negatively controlled during the cleavage stage of development, which is characterized by rapid mitosis. Later in development, Reps1 is required for the normal function of the ectodermic cell, and its targeting into the plasma membrane affects the stability of the ectoderm.
authors.affiliation: Institut Jacques Monod, CNRS (UMR7592), Université Paris Diderot, Paris, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Boissel
authors.forename: Jonathan
authors.initials: J
authors.lastname: Fillatre
authors.forename: Jacques
authors.initials: J
authors.lastname: Moreau
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D047108:Embryonic Development / Q000235:genetics; D004705:Endocytosis; D018507:Gene Expression Regulation, Developmental; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008954:Models, Biological; D009154:Mutation; D010641:Phenotype; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D021381:Protein Transport; D029867:Xenopus Proteins / Q000235:genetics / Q000378:metabolism*; D014982:Xenopus laevis
chemical_list: D002135:Calcium-Binding Proteins; D002352:Carrier Proteins; D008565:Membrane Proteins; C500422:Ralbp1 protein, Xenopus; D029867:Xenopus Proteins; C574013:reps1 protein, Xenopus