title: Distribution of Chlamydia trachomatis serotypes in clinical urogenital samples from north-eastern Croatia.
abstract: The purpose of this study was to determine prevalence of Chlamydia trachomatis (Ct) urogenital infection and its serotype distribution from clinical samples in north-eastern Croatia. During a 3-year period, 2,379 urogenital samples were analyzed by real-time polymerase chain reaction (A group), while 4,846 genital swabs were analyzed by direct fluorescent antibody test (B group). 132 Ct positive specimens were genotyped by omp1 gene sequencing. The prevalence rate of Ct was 3.2 % in A and 1 % in B group. The most prevalent chlamydial genotype was E (44 %), followed by F (33 %), K (11.5 %), G (8 %), J/UW (5.3 %), D-IC (4.4 %), D-B120 (1.8 %), and B/IU, J/IU, Ia/IU (0.9 % each) serotypes. Single-nucleotide polymorphisms (SNPs) of omp1 gene were detected in E, K, and G serotypes. Some of these SNPs (C/T at position 272 and G/A at position 813 in E strain; C/T at position 884 in D strain) might represent novel omp1 variants.
authors.affiliation: Department of Microbiology, Institute of Public Health Osijek, Osijek, Croatia. zinkahr@gmail.com
authors.forename: Zinka
authors.initials: Z
authors.lastname: Bošnjak
authors.forename: Snježana
authors.initials: S
authors.lastname: Džijan
authors.forename: Dinko
authors.initials: D
authors.lastname: Pavlinić
authors.forename: Magdalena
authors.initials: M
authors.lastname: Perić
authors.forename: Nataša
authors.initials: N
authors.lastname: Ružman
authors.forename: Ivana Roksandić
authors.initials: IR
authors.lastname: Križan
authors.forename: Gordan
authors.initials: G
authors.lastname: Lauc
authors.forename: Arlen
authors.initials: A
authors.lastname: Antolović-Požgain
authors.forename: Jelena
authors.initials: J
authors.lastname: Burazin
authors.forename: Dubravka
authors.initials: D
authors.lastname: Vuković
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002692:Chlamydia trachomatis / Q000145:classification* / Q000302:isolation & purification*; D017523:Croatia / Q000453:epidemiology; D005260:Female; D019085:Fluorescent Antibody Technique, Direct; D005835:Genitalia / Q000382:microbiology; D005838:Genotype; D006801:Humans; D008219:Lymphogranuloma Venereum / Q000453:epidemiology* / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D018272:Porins / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D016036:Seroepidemiologic Studies; D012703:Serotyping; D014556:Urine / Q000382:microbiology; D055815:Young Adult
chemical_list: D018272:Porins; C071677:omp1 protein, Chlamydia trachomatis