title: HIV-1 proviral DNA loads (as determined by quantitative PCR) in patients subjected to structured treatment interruption after antiretroviral therapy failure.
abstract: The impact of Structured Treatment Interruption (STI) in peripheral blood mononuclear cell (PBMC) proviral reservoirs in 41 highly active antiretroviral therapy (HAART)-treated viremic individuals at baseline and 12 weeks after STI was determined using quantitative PCR (qPCR). Viral load increased 0.7 log(10) and CD4 decreased 97.5 cells/mm(3) after 12 weeks. A total of 28 of the 41 individuals showed an increased proviral load, 19 with a statistically significant increase above 10%. An increase in active viral replication is an important factor in the replenishment of the proviral reservoir even for short time periods.
authors.affiliation: Retrovirology Laboratory, Infectious Diseases Division, Federal University of São Paulo (UNIFESP), São Paulo, Brazil. skomninakis@yahoo.com.br
authors.forename: Shirley V
authors.initials: SV
authors.lastname: Komninakis
authors.forename: Domingos E M
authors.initials: DE
authors.lastname: Santos
authors.forename: Carlos
authors.initials: C
authors.lastname: Santos
authors.forename: Márcia P R
authors.initials: MP
authors.lastname: Oliveros
authors.forename: Sabri
authors.initials: S
authors.lastname: Sanabani
authors.forename: Ricardo S
authors.initials: RS
authors.lastname: Diaz
mesh_terms: D000328:Adult; D019380:Anti-HIV Agents / Q000008:administration & dosage*; D023241:Antiretroviral Therapy, Highly Active / Q000379:methods*; D018791:CD4 Lymphocyte Count; D002478:Cells, Cultured; D004279:DNA, Viral / Q000302:isolation & purification*; D005260:Female; D015658:HIV Infections / Q000188:drug therapy* / Q000821:virology; D015497:HIV-1 / Q000302:isolation & purification; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology; D008297:Male; D008875:Middle Aged; D011533:Proviruses / Q000302:isolation & purification*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D017211:Treatment Failure; D019562:Viral Load; D028761:Withholding Treatment
chemical_list: D019380:Anti-HIV Agents; D004279:DNA, Viral