title: Microglia-like or microglia: results of the weak silver carbonate staining method of del Rio-Hortega.
abstract: We investigated the character and origin of the newly described "microglia-like" cell in the mammalian organ of Corti after aminoglycoside intoxication. We replicated the neomycin-induced ototoxicity model in cochleae of neonatal Sprague-Dawley rats and used their brains as microglia positive controls. The weak silver carbonate staining method of del Rio-Hortega was used to identify the microglia-like cell. The microglia-like cell was confirmed by transmission electron microscope (TEM). Microglia in the brain were stained while rat microglia-like cells in the cochlea were unstained by the weak silver carbonate staining method of del Rio-Hortega. Because the microglia-like cell was unstained by the method of del Rio-Hortega, it is unlikely that the newly found cell is related to microglia.
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Eye and ENT Hospital of Fudan University, China.
authors.forename: Yucheng
authors.initials: Y
authors.lastname: Wang
authors.forename: W
authors.initials: W
authors.lastname: Wei
mesh_terms: D000818:Animals; D002254:Carbonates / Q000737:chemistry*; D005260:Female; D008297:Male; D017628:Microglia / Q000737:chemistry* / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D009925:Organ of Corti / Q000166:cytology / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D018030:Silver Compounds / Q000737:chemistry*; D016622:Silver Staining
chemical_list: D002254:Carbonates; D018030:Silver Compounds; C033260:silver carbonate