title: Superovulation of beef cattle with a split-single intramuscular administration of Folltropin-V in two concentrations of hyaluronan.
abstract: Three experiments were designed to evaluate the superovulatory response of beef cows following two intramuscular (IM) administrations 48 h apart of Folltropin-V diluted in reduced concentrations of hyaluronan (Split-single IM administrations; Experiment 1-300 mg Folltropin-V on the first day and 100 mg 48 h later; Experiment 2-200 mg Folltropin-V on the first day and 100 mg 48 h later). In Experiments 1 and 2, superovulatory response and ova embryo/embryo production did not differ between donors receiving twice daily IM of Folltropin-V over 4 days and those given a Split-single IM administration of Folltropin-V diluted in 10 mg/mL hyaluronan solution. Experiment 3 compared Split-single IM administration of Folltropin-V diluted in two hyaluronan concentrations (5 or 10 mg/mL) with Folltropin-V diluted in saline and administered twice-daily over 4 days. Beef cows (17 Angus and 12 Simmental) were randomly assigned to one of three treatment groups to be superstimulated three times in a cross-over design, so that all cows received all treatments. A total dose of 300 mg Folltropin-V was divided into twice-daily IM over 4 days, or in two IM treatment 48 h apart (200 mg on first day and 100 mg 48 h later) in the hyaluronan groups. Mean (± SEM) numbers of transferable embryos did not differ among treatment groups (Control: 4.0 ± 0.8; 10 mg/mL hylauronan: 5.0 ± 0.9; 5 mg/mL hyaluronan: 6.1 ± 1.3). We concluded that the Split-single IM administration of Folltropin-V diluted in either concentration of hyaluronan resulted in a comparable superovulatory response to the traditional twice-daily protocol.
authors.affiliation: Instituto de Reproducción Animal Córdoba (IRAC), Zona Rural General Paz, (5145) Córdoba, Argentina.
authors.forename: Andrés
authors.initials: A
authors.lastname: Tríbulo
authors.forename: Dragan
authors.initials: D
authors.lastname: Rogan
authors.forename: Humberto
authors.initials: H
authors.lastname: Tríbulo
authors.forename: Ricardo
authors.initials: R
authors.lastname: Tríbulo
authors.forename: Reuben J
authors.initials: RJ
authors.lastname: Mapletoft
authors.forename: Gabriel A
authors.initials: GA
authors.lastname: Bó
mesh_terms: D000818:Animals; D002417:Cattle; D005260:Female; D005640:Follicle Stimulating Hormone / Q000008:administration & dosage / Q000494:pharmacology*; D007273:Injections, Intramuscular / Q000662:veterinary; D006080:Ovarian Follicle / Q000000981:diagnostic imaging / Q000187:drug effects; D010060:Ovulation / Q000187:drug effects*; D010062:Ovulation Induction / Q000379:methods / Q000662:veterinary*; D013480:Superovulation / Q000187:drug effects*; D014463:Ultrasonography
chemical_list: D005640:Follicle Stimulating Hormone