title: Examining the potential of plasma-assisted pretreated wheat straw for enzyme production by Trichoderma reesei.
abstract: Plasma-assisted pretreated wheat straw was investigated for cellulase and xylanase production by Trichoderma reesei fermentation. Fermentations were conducted with media containing washed and unwashed plasma-assisted pretreated wheat straw as carbon source which was sterilized by autoclavation. To account for any effects of autoclavation, a comparison was made with unsterilized media containing antibiotics. It was found that unsterilized washed plasma-assisted pretreated wheat straw (which contained antibiotics) was best suited for the production of xylanases (110 IU ml(-1)) and cellulases (0.5 filter paper units (FPU) ml(-1)). Addition of Avicel boosted enzyme titers with the highest cellulase titers (1.5 FPU ml(-1)) found with addition of 50 % w/w Avicel and with the highest xylanase production (350 IU ml(-1)) reached in the presence of 10 % w/w Avicel. Comparison with enzyme titers from other nonrefined feedstocks suggests that plasma pretreated wheat straw is a promising and suitable substrate for cellulase and hemicellulase production.
authors.affiliation: Center for Microbial Biotechnology, Department of Systems Biology, Technical University of Denmark, Kgs Lyngby, Denmark.
authors.forename: Divanery
authors.initials: D
authors.lastname: Rodriguez-Gomez
authors.forename: Linda
authors.initials: L
authors.lastname: Lehmann
authors.forename: Nadja
authors.initials: N
authors.lastname: Schultz-Jensen
authors.forename: Anne Belinda
authors.initials: AB
authors.lastname: Bjerre
authors.forename: Timothy John
authors.initials: TJ
authors.lastname: Hobley
mesh_terms: D000085:Acetates / Q000737:chemistry; D002241:Carbohydrates / Q000737:chemistry; D002480:Cellulase / Q000096:biosynthesis*; D043364:Endo-1,4-beta Xylanases / Q000096:biosynthesis*; D005285:Fermentation; D058626:Plasma Gases / Q000737:chemistry*; D014242:Trichoderma / Q000378:metabolism*; D014908:Triticum / Q000737:chemistry*
chemical_list: D000085:Acetates; D002241:Carbohydrates; D058626:Plasma Gases; D002480:Cellulase; D043364:Endo-1,4-beta Xylanases