title: [Screening and identification of receptor reacting with nucleocapsid protein of duck enteritis virus].
abstract: To clarify the pathogenesis of Duck enteritis virus (DEV), the cDNA library of duck's liver infected by DEV and a bait plasmid containing DEV nucleocapsid protein (NP) gene were constructed, then the receptor was screened from the cDNA library plasmid by the yeast two-hybrid system and verified by GST pull-down test. The results showed that the capacity of the primary cDNA library was 1 x 106 CFU with insertion size from 0.5 to 1 kb, and the bait plasmid of pGBKT7-NP showed no self-activation. The receptor reacting with DEV NP in duck liver was initially confirmed as the protein kinase C inhibitor (PKCI). These results provide new clues for further investigation on pathogenesis of DEV.
authors.affiliation: College of Animal Science, Guizhou University, Guiyang, Guizhou 550025, China. hezhang514@yahoo.cn
authors.forename: He
authors.initials: H
authors.lastname: hang
authors.forename: Jun-Ting
authors.initials: JT
authors.lastname: Mao
authors.forename: Ying
authors.initials: Y
authors.lastname: Yang
authors.forename: Kai-Gong
authors.initials: KG
authors.lastname: Wang
authors.forename: Bi-Jun
authors.initials: BJ
authors.lastname: Zhou
authors.forename: Ming
authors.initials: M
authors.lastname: Wen
mesh_terms: D018138:Alphaherpesvirinae / Q000472:pathogenicity*; D000818:Animals; D004372:Ducks / Q000821:virology*; D015723:Gene Library; D008099:Liver / Q000821:virology; D019590:Nucleocapsid Proteins / Q000235:genetics*; D010957:Plasmids; D011991:Receptors, Virus / Q000032:analysis*; D020798:Two-Hybrid System Techniques
chemical_list: D019590:Nucleocapsid Proteins; D011991:Receptors, Virus