title: [Obtaining of hairy-root, callus and suspension carrot culture (Daucus carota L.) able to accumulate human interferon alpha-2b].
abstract: Here we report the obtaining of suspension, callus and hairy root culture initiated from carrot plants of Nantskaya and Perfektzya variety with the highest level of recombinant human interferon alpha-2b accumulation exhibited the highest level of plant protein extract antiviral activity (up to 12.8 x 10(3) IU/mg TSP). The antiviral activity of callus extracts was significantly lower comparing to the activity of plant extracts from parent organisms. However, the antiviral activity level of suspension culture extracts (up to 4.42 x 10(3) IU/mg TSP) and Ri-root ones (up to 4.42 x 10(3) IU/mg TSP) appeared to be comparable to analogical data of antiviral activity of transgenic carrot leaf extracts, this way the described cultures could be possibly used for comparatively speedy obtaining of recombinant therapeutic protein for curing and preventing of virus diseases.
authors.forename: Iu S
authors.initials: IuS
authors.lastname: Luchakivskaia
authors.forename: Z M
authors.initials: ZM
authors.lastname: Olevinskaia
authors.forename: E M
authors.initials: EM
authors.lastname: Kishchenko
authors.forename: N Ia
authors.initials: NIa
authors.lastname: Spivak
authors.forename: N V
authors.initials: NV
authors.lastname: Kuchuk
mesh_terms: D000998:Antiviral Agents / Q000378:metabolism; D002478:Cells, Cultured; D018552:Daucus carota / Q000235:genetics / Q000378:metabolism*; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000096:biosynthesis* / Q000235:genetics; D010936:Plant Extracts / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000378:metabolism; D030821:Plants, Genetically Modified; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014777:Virus Diseases / Q000188:drug therapy
chemical_list: D000998:Antiviral Agents; D000077190:Interferon alpha-2; D016898:Interferon-alpha; D010936:Plant Extracts; D011994:Recombinant Proteins