title: Fluorescence correlation spectroscopy.
abstract: Fluorescence correlation spectroscopy (FCS) is a powerful technique to measure concentrations, mobilities, and interactions of fluorescent biomolecules. It can be applied to various biological systems such as simple homogeneous solutions, cells, artificial, or cellular membranes and whole organisms. Here, we introduce the basic principle of FCS, discuss its application to biological questions as well as its limitations and challenges, present an overview of novel technical developments to overcome those challenges, and conclude with speculations about the future applications of fluorescence fluctuation spectroscopy.
authors.affiliation: Institute of Biochemistry, ETH Zurich, Zurich, Switzerland.
authors.forename: Jonas
authors.initials: J
authors.lastname: Ries
authors.forename: Petra
authors.initials: P
authors.lastname: Schwille
mesh_terms: D005456:Fluorescent Dyes / Q000032:analysis; D008962:Models, Theoretical; D057054:Molecular Imaging / Q000379:methods*; D011506:Proteins / Q000032:analysis*; D012996:Solutions; D013050:Spectrometry, Fluorescence / Q000379:methods* / Q000639:trends*; D051598:Whole Body Imaging
chemical_list: D005456:Fluorescent Dyes; D011506:Proteins; D012996:Solutions