title: A silicon dioxide modified magnetic nanoparticles-labeled lateral flow strips for HBs antigen.
abstract: Herein we reported a new type of silicon dioxide wrapped magnetic nanoparticles-labeled lateral flow strip for detection of HBs antigen in sera. The SiO2 wrapped Fe3O4 nanocomposites were prepared and characterized by HR-TEM, FTIR and magnetometer. As-prepared nanocomposites were used to label anti-HBV surface monoclonal antibody, the lateral flow strips were constructed, and 100 specimens of sera were collected and tested. Results showed that the prepared SiO2 wrapped Fe3O4 nanocomposites were shell/core structure, well dispersed, with the size of 25 nm in diameter, the thickness of the shell was about 3 nm, their magnetic saturation intensity was 44.3 meu g(-1). Clinical sera specimens test results showed that the prepared lateral flow strips were with the detection limitation of 5 pg/mL by naked eye observation, and 0.1 pg/mL by CCD reader or MAR Analyzer, specificity was 100%. In conclusion, one kind of silicon dioxide wrapped magnetic nanoparticles-labeled lateral flow strip for ultrasensitive detection of HBs antigen was successfully developed, its ease of use, sensitiveness and low-cost make it well-suited for population-based on-the-site hepatitis B screening.
authors.affiliation: Department of Bio-Nano-Science and Engineering, National Key Laboratory of Nano/Micro Fabrication Technology, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Institute of Micro-Nano Science and Technology, Shanghai JiaoTong University, 800 Dongchuan Road, Shanghai 200240, China.
authors.forename: Xueqing
authors.initials: X
authors.lastname: Zhang
authors.forename: Lin
authors.initials: L
authors.lastname: Jiang
authors.forename: Chunlei
authors.initials: C
authors.lastname: Zhang
authors.forename: Ding
authors.initials: D
authors.lastname: Li
authors.forename: Can
authors.initials: C
authors.lastname: Wang
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.forename: Daxiang
authors.initials: D
authors.lastname: Cui
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry; D006509:Hepatitis B / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D006510:Hepatitis B Antibodies / Q000737:chemistry; D006514:Hepatitis B Surface Antigens / Q000097:blood*; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D053761:Nanocomposites / Q000737:chemistry*; D010316:Particle Size; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D000911:Antibodies, Monoclonal; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D058185:Magnetite Nanoparticles; D011933:Reagent Kits, Diagnostic; D012822:Silicon Dioxide