title: Inactivation of stable viruses in cell culture facilities by peracetic acid fogging.
abstract: Looking for a robust and simple method to replace formaldehyde fumigation for the disinfection of virus-handling laboratories and facilities, we tested peracetic acid fogging as a method to inactivate stable viruses under practical conditions. Peracetic acid/hydrogen peroxide (5.8%/27.5%, 2.0 mL/m³) was diluted in sufficient water to achieve ≥ 70% relative humidity and was vaporized as <10 μm droplets in a fully equipped 95 m³ laboratory unit. High titers of reovirus 3, MVM parvovirus and an avian polyomavirus were coated on frosted glass carriers and were exposed to the peracetic acid fog in various positions in the laboratory. After vaporization, a 60 min exposure time, and venting of the laboratory, no residual virus was detected on any of the carriers (detection limit <1 infectious unit/sample volume tested). The log reduction values were 9.0 for reovirus, 6.4 for MVM parvovirus, and 7.65 for the polyomavirus. After more than 10 disinfection runs within 12 months, no damage or functional impairment of electrical and electronic equipment was noted.
authors.affiliation: Novartis Vaccines and Diagnostics GmbH, Emil-von-Behring-Str. 76, D-35041 Marburg, Germany. jens-peter.gregersen@novartis.com
authors.forename: Jens-Peter
authors.initials: JP
authors.lastname: Gregersen
authors.forename: Bernhard
authors.initials: B
authors.lastname: Roth
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D010463:Peracetic Acid / Q000494:pharmacology*; D011120:Polyomavirus / Q000187:drug effects*; D038301:Virus Inactivation
chemical_list: D010463:Peracetic Acid