title: [Effects of high magneto-gravitational environment on calcium/calmodulin signal of MG63 osteoblast-like cells].
abstract: OBJECTIVE
  To investigate the effects of high magneto-gravitational environment on Ca(2+);]/calmodulin (CaM) signal of MG63 osteoblast-like cells.
  
  
  METHODS
  A special designed large gradient high magnetic field could produce three different high magneto-gravitational environments including μg (12 T),  1 g (16 T) and 2 g (12 T). The effects of high magneto-gravitational environments on intracellular free Ca(2+);] concentration ([Ca(2+);](i);) and protein expression including calmodulin (CaM),  myosin light chain kinases (MLCK) and phosphorylated Ca(2+);]/CaM dependent protein kinase II(pCaMKII) were measured by Fluo-3/AM or Western blot,  respectively.
  
  
  RESULTS
  When compared with control group,  an increase of [Ca(2+);](i); of MG63 was caused by strong magnetic field;   Compared to 2 g,  μg decreased [Ca(2+);](i); of MG63. The protein expression of CaM and pCaMKIIof MG63 cells was decreased by simulated weightlessness.
  
  
  CONCLUSIONS
  [Ca(2+);](i); of MG63 cells was increased by strong magnetic field;   simulated weightlessness inhibited Ca(2+);/CaM signaling of MG63 cells.
authors.affiliation: School of Life Sciences, Northwestern Polytechnical University, Xi'an, China.
authors.forename: Ming-zhi
authors.initials: MZ
authors.lastname: Luo
authors.forename: Sheng-sheng
authors.initials: SS
authors.lastname: Li
authors.forename: Xiang
authors.initials: X
authors.lastname: Gao
authors.forename: Yi
authors.initials: Y
authors.lastname: Lu
authors.forename: Zhou-qi
authors.initials: ZQ
authors.lastname: Yang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Peng
authors.initials: P
authors.lastname: Shang
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000378:metabolism; D002147:Calmodulin / Q000378:metabolism*; D045744:Cell Line, Tumor; D004789:Enzyme Activation; D005786:Gene Expression Regulation; D006112:Gravitation; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism; D060328:Magnetic Phenomena; D010006:Osteoblasts / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D002147:Calmodulin; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D002118:Calcium