title: Oxidative DNA damage and global DNA hypomethylation are related to folate deficiency in chromate manufacturing workers.
abstract: Exposure to hexavalent chromium [Cr (VI)] can cause DNA damage, genetic instability and increase the risk of cancer development. Folate deficiency affects DNA methylation and reduces the stability of the genetic material. However, the correlation between folate deficiency and DNA damage has never been clearly elucidated in chromate workers. In this study, we recruited one hundred and fifteen workers from chromate producing facilities as testing subjects and sixty local residents without chromium exposure history served as controls. The results showed an evident accumulation of Cr in peripheral red blood cells accompanied by a significantly decreased serum folate in chromate exposed workers. The decreased serum folate was associated with an increased urinary 8-hydroxy-2'-deoxyguanosine, DNA strand breaks and global DNA hypomethylation. These findings suggest that chronic occupational chromate exposure could induce folate depletion, which may further promote DNA damages and global DNA hypomethylation. Adequate folate supplement may provide benefit to chromate sufferers in stabilization of genetic material and reduce the risk of cancer development.
authors.affiliation: Department of Clinical Laboratories, Third Hospital of Peking University, Beijing 100191, China.
authors.forename: Tian-Cheng
authors.initials: TC
authors.lastname: Wang
authors.forename: Yan-Shuang
authors.initials: YS
authors.lastname: Song
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Ji
authors.initials: J
authors.lastname: Zhang
authors.forename: Shan-Fa
authors.initials: SF
authors.lastname: Yu
authors.forename: Yong-En
authors.initials: YE
authors.lastname: Gu
authors.forename: Tian
authors.initials: T
authors.lastname: Chen
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
authors.forename: Hui-Qi
authors.initials: HQ
authors.lastname: Shen
authors.forename: Guang
authors.initials: G
authors.lastname: Jia
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000395:Air Pollutants, Occupational / Q000032:analysis; D016902:Air Pollution, Indoor / Q000032:analysis; D002681:China; D002840:Chromates / Q000009:adverse effects* / Q000097:blood / Q000652:urine; D020552:Comet Assay; D053960:DNA Breaks / Q000187:drug effects; D004249:DNA Damage; D019175:DNA Methylation / Q000187:drug effects*; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000652:urine; D004912:Erythrocytes / Q000737:chemistry; D005260:Female; D005492:Folic Acid / Q000097:blood; D005494:Folic Acid Deficiency / Q000139:chemically induced* / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000097:blood; D006710:Homocysteine / Q000097:blood; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D016273:Occupational Exposure / Q000009:adverse effects*; D010084:Oxidation-Reduction; D013482:Superoxide Dismutase / Q000097:blood
chemical_list: D000395:Air Pollutants, Occupational; D002840:Chromates; D007202:Indicators and Reagents; D006710:Homocysteine; D008315:Malondialdehyde; D000080242:8-Hydroxy-2'-Deoxyguanosine; D005492:Folic Acid; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D003849:Deoxyguanosine