title: A method for simultaneous echo planar imaging of hyperpolarized ¹³C pyruvate and ¹³C lactate.
abstract: A rapid echo planar imaging sequence for dynamic imaging of [1-(13)C] lactate and [1-(13)C] pyruvate simultaneously was developed. Frequency-based separation of these metabolites was achieved by spatial shifting in the phase-encoded direction with the appropriate choice of echo spacing. Suppression of the pyruvate-hydrate and alanine resonances is achieved through an optimized spectral-spatial RF waveform. Signal sampling efficiency as a function of pyruvate and lactate excitation angle was simulated using two site exchange models. Dynamic imaging is demonstrated in a transgenic mouse model, and phantom validations of the RF pulse frequency selectivity were performed.
authors.affiliation: Department of Radiology and Biomedical Imaging, University of California San Francisco, San Francisco, CA, USA. galen.reed@ucsf.edu
authors.forename: Galen D
authors.initials: GD
authors.lastname: Reed
authors.forename: Peder E Z
authors.initials: PE
authors.lastname: Larson
authors.forename: Cornelius von
authors.initials: Cv
authors.lastname: Morze
authors.forename: Robert
authors.initials: R
authors.lastname: Bok
authors.forename: Michael
authors.initials: M
authors.lastname: Lustig
authors.forename: Adam B
authors.initials: AB
authors.lastname: Kerr
authors.forename: John M
authors.initials: JM
authors.lastname: Pauly
authors.forename: John
authors.initials: J
authors.lastname: Kurhanewicz
authors.forename: Daniel B
authors.initials: DB
authors.lastname: Vigneron
mesh_terms: D000818:Animals; D002247:Carbon Isotopes / Q000378:metabolism; D045744:Cell Line, Tumor; D017352:Echo-Planar Imaging / Q000379:methods*; D019344:Lactic Acid / Q000378:metabolism*; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology*; D019289:Pyruvic Acid / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D002247:Carbon Isotopes; D019344:Lactic Acid; D019289:Pyruvic Acid