title: Structural changes in marinated fillets of gilthead sea bream (Sparus aurata L.) during vacuum storage at refrigerated temperature.
abstract: Fillets of Sparus aurata were marinated in a mixture of salt (5.5%) and citric acid (48.9 g/kg) for 1 h, then vacuum packed and refrigerated for 1, 5, 16, and 21 d. Structural and ultrastructural parameters were evaluated in 6 specimens per time period. At day 1, the preservative solution appeared among the muscle fibers, thus increasing the interfibrillar spaces; cytoplasmic organelles were swollen and the sarcolemma-endomysium appeared very altered and electron-dense. At 5 to 10 d, the marinade solution had penetrated into the muscle fibers. The subsarcolemmal and intermyofibrillar spaces were dilated and granular lines of electron-dense material appeared among the fibers. From 16 d, broad interfibrillar spaces were occupied by granular material derived from denatured sarcolemma-endomysium and denatured myofibrils. Sarcomeres were already altered from initial stages, mainly at the I-band level. The Z-line also appeared disrupted. Such alterations were more severe from 10 d on, such that most of the sarcomeres showed disintegration of myofilaments.
authors.affiliation: Dept. Anatomía y Anatomía Patológica Comparadas, Facultad de Veterinaria, Univ. de Murcia, 30100, Spain. mdayala@um.es
authors.forename: María D
authors.initials: MD
authors.lastname: Ayala
authors.forename: Francisco
authors.initials: F
authors.lastname: Gil
authors.forename: José M
authors.initials: JM
authors.lastname: Vázquez
authors.forename: Rafael
authors.initials: R
authors.lastname: Latorre
authors.forename: Gregorio
authors.initials: G
authors.lastname: Ramírez
authors.forename: Octavio
authors.initials: O
authors.lastname: López
mesh_terms: D000818:Animals; D061353:Food Storage / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008460:Meat / Q000032:analysis*; D009210:Myofibrils / Q000737:chemistry / Q000648:ultrastructure; D011489:Protein Denaturation / Q000187:drug effects; D059748:Proteolysis; D012034:Refrigeration; D012508:Sarcolemma / Q000378:metabolism; D021541:Sea Bream; D014618:Vacuum