title: Allergen-specific responses of CD19(+)CD5(+)Foxp3(+) regulatory B cells (Bregs) and CD4(+)Foxp3(+) regulatory T cell (Tregs) in immune tolerance of cow milk allergy of late eczematous reactions.
abstract: Foxp3-expressing cells among CD19(+)CD5(+) B cells were identified as regulatory B cells. Food allergy manifesting as late eczematous reactions is regarded as a non-IgE-mediated food allergy. The diagnosis for milk allergy manifesting as late eczematous reactions was made on the basis of the findings obtained from a double-blind placebo-controlled food challenge in patients with atopic dermatitis. Twelve patients with milk allergy and 12 patients who could tolerate milk were selected. On casein stimulation, the CD19(+)CD5(+)Foxp3(+) B cell (Breg) fraction in CD5(+) B cells decreased from 4.4±1.1% to 3.1±0.7% (P=0.047, n=12) in the milk allergy group and increased from 4.4±1.3% to 5.2±1.4% (P=0.001, n=10) in the milk-tolerant group. On the other hand, on allergen stimulation, the number of CD4(+)Foxp3(+) regulatory T cells (Tregs) in the milk allergy group and milk-tolerant group increased from 2.6±0.7% to 3.4±0.6% (P=0.014, n=9) and from 2.7±1.0% to 3.5±1.0% (P=0.038, n=10), respectively. In conclusion, allergen-specific responses of Bregs, rather than those of Tregs, seem to influence the immune responses (i.e., allergy or tolerance) to a food allergen.
authors.affiliation: Department of Animal Biotechnology, College of Animal Bioscience & Technology, Konkuk University, Seoul, Republic of Korea.
authors.forename: Joonyong
authors.initials: J
authors.lastname: Noh
authors.forename: Geunwoong
authors.initials: G
authors.lastname: Noh
authors.forename: Hyuk Soon
authors.initials: HS
authors.lastname: Kim
authors.forename: A-Ram
authors.initials: AR
authors.lastname: Kim
authors.forename: Wahn Soo
authors.initials: WS
authors.lastname: Choi
mesh_terms: D000293:Adolescent; D000818:Animals; D018941:Antigens, CD19 / Q000032:analysis; D060151:B-Lymphocytes, Regulatory / Q000276:immunology*; D018956:CD5 Antigens / Q000032:analysis; D002364:Caseins / Q000276:immunology; D049109:Cell Proliferation; D002478:Cells, Cultured; D002648:Child; D004485:Eczema / Q000276:immunology*; D005260:Female; D051858:Forkhead Transcription Factors / Q000032:analysis; D006801:Humans; D008297:Male; D008892:Milk / Q000276:immunology; D016269:Milk Hypersensitivity / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
chemical_list: D018941:Antigens, CD19; D018956:CD5 Antigens; D002364:Caseins; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors