title: Acute effect of high-dose isoflavones from Pueraria lobata (Willd.) Ohwi on lipid and bone metabolism in ovariectomized mice.
abstract: We investigated the acute metabolic effects of isoflavones from Pueraria lobata (Willd.) Ohwi (IPL) in ovariectomized (OVX) mice. After 4 weeks of IPL feeding at 500 mg/day/kg body weight (OVX500), plasma 17β-estradiol concentrations were significantly higher (+25%, p < 0.05), whereas plasma triglyceride levels were significantly lower in OVX mice (-15%, p < 0.05) compared with controls. Abdominal adipose tissue weight was marginally reduced in IPL-fed groups compared with OVX controls and the plasma levels of liver enzymes were unchanged. In addition, IPL significantly inhibited the reduction of bone mineral density in the femurs of OVX mice (OVX200, +22%; OVX500, +26%; p < 0.05) compared with controls after 4 weeks of IPL feeding. In quantitative polymerase chain reaction analysis the expression of aromatase was significantly suppressed and SULT1E1 was increased by IPL feeding, showing that IPL feeding may not alter the risk for breast cancer in mice. Our results suggest that IPL could ameliorate menopausal symptoms in mice. Further studies will confirm the effects of IPL in humans.
authors.affiliation: Division of Food Bioscience and Technology, College of Life Sciences and Biotechnology, Korea University, Seoul, 136-713, Korea.
authors.forename: Hee Joon
authors.initials: HJ
authors.lastname: Cho
authors.forename: Hee-jin
authors.initials: HJ
authors.lastname: Jun
authors.forename: Ji Hae
authors.initials: JH
authors.lastname: Lee
authors.forename: Yaoyao
authors.initials: Y
authors.lastname: Jia
authors.forename: Minh Hien
authors.initials: MH
authors.lastname: Hoang
authors.forename: Jae-Hoon
authors.initials: JH
authors.lastname: Shim
authors.forename: Kwan-Hwa
authors.initials: KH
authors.lastname: Park
authors.forename: Sung-Joon
authors.initials: SJ
authors.lastname: Lee
mesh_terms: D000818:Animals; D015519:Bone Density / Q000187:drug effects*; D050171:Dyslipidemias / Q000188:drug therapy; D004958:Estradiol / Q000097:blood; D005260:Female; D005269:Femur / Q000187:drug effects; D007529:Isoflavones / Q000494:pharmacology*; D050356:Lipid Metabolism / Q000187:drug effects*; D008593:Menopause / Q000187:drug effects; D051379:Mice; D008813:Mice, Inbred ICR; D009929:Organ Size / Q000187:drug effects; D010024:Osteoporosis / Q000188:drug therapy; D010052:Ovariectomy; D029908:Pueraria / Q000737:chemistry*; D014280:Triglycerides / Q000097:blood
chemical_list: D007529:Isoflavones; D014280:Triglycerides; D004958:Estradiol; C033607:puerarin