title: Borondipyrromethene-derived Cu2+ sensing chemodosimeter for fast and selective detection.
abstract: Here, we report a new Cu(2+)-selective fluorescent turn-on probe BODIPY-EP, in which the 2-pyridinecarboxylic acid is connected to a 6-hydroxyindole-based BODIPY platform through an ester linkage. The ester bond of BODIPY-EP is selectively hydrolyzed by the reaction with Cu(2+) under mild and neutral conditions to generate BODIPY-OH, showing strong characteristic fluorescence of BODIPY-OH. The favorable features of BODIPY-EP towards Cu(2+) include fast response, large fluorescence enhancement and high selectivity. We further demonstrated that the membrane-permeable probe reacts with intracellular Cu(2+) and exhibits bright fluorescence in living cells.
authors.affiliation: Key Laboratory for Advanced Materials and Institute of Fine Chemicals, East China University of Science & Technology, Shanghai 200237, PR China. zhaocchang@ecust.edu.cn
authors.forename: Chunchang
authors.initials: C
authors.lastname: Zhao
authors.forename: Peng
authors.initials: P
authors.lastname: Feng
authors.forename: Jian
authors.initials: J
authors.lastname: Cao
authors.forename: Xuzhe
authors.initials: X
authors.lastname: Wang
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.forename: Yulin
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jinxing
authors.initials: J
authors.lastname: Zhang
authors.forename: Yanfen
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D001896:Boron Compounds / Q000138:chemical synthesis*; D002413:Cations, Divalent; D002463:Cell Membrane Permeability; D003300:Copper / Q000032:analysis*; D003600:Cytosol / Q000737:chemistry; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D057809:HEK293 Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008958:Models, Molecular; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D002413:Cations, Divalent; D005456:Fluorescent Dyes; D003300:Copper