title: Immunosensor towards low-cost, rapid diagnosis of tuberculosis.
abstract: A rapid, accurate tuberculosis diagnostic tool that is compatible with the needs of tuberculosis-endemic settings is a long-sought goal. An immunofluorescence microtip sensor is described that detects Mycobacterium tuberculosis complex cells in sputum in 25 minutes. Concentration mechanisms based on flow circulation and electric field are combined at different scales to concentrate target bacteria in 1 mL samples onto the surfaces of microscale tips. Specificity is conferred by genus-specific antibodies on the microtip surface. Immunofluorescence is then used to detect the captured cells on the microtip. The detection limit in sputum is 200 CFU mL(-1) with a success rate of 96%, which is comparable to PCR.
authors.affiliation: Department of Mechanical Engineering, University of Washington, Box 352600, Seattle, Washington 98195, USA.
authors.forename: Jong-Hoon
authors.initials: JH
authors.lastname: Kim
authors.forename: Woon-Hong
authors.initials: WH
authors.lastname: Yeo
authors.forename: Zhiquan
authors.initials: Z
authors.lastname: Shu
authors.forename: Scott D
authors.initials: SD
authors.lastname: Soelberg
authors.forename: Shinnosuke
authors.initials: S
authors.lastname: Inoue
authors.forename: Dinesh
authors.initials: D
authors.lastname: Kalyanasundaram
authors.forename: John
authors.initials: J
authors.lastname: Ludwig
authors.forename: Clement E
authors.initials: CE
authors.lastname: Furlong
authors.forename: James J
authors.initials: JJ
authors.lastname: Riley
authors.forename: Kris M
authors.initials: KM
authors.lastname: Weigel
authors.forename: Gerard A
authors.initials: GA
authors.lastname: Cangelosi
authors.forename: Kieseok
authors.initials: K
authors.lastname: Oh
authors.forename: Kyong-Hoon
authors.initials: KH
authors.lastname: Lee
authors.forename: Dayong
authors.initials: D
authors.lastname: Gao
authors.forename: Jae-Hyun
authors.initials: JH
authors.lastname: Chung
mesh_terms: D015374:Biosensing Techniques / Q000191:economics / Q000295:instrumentation; D004867:Equipment Design; D005455:Fluorescent Antibody Technique / Q000191:economics / Q000295:instrumentation*; D006801:Humans; D057230:Limit of Detection; D046210:Microfluidic Analytical Techniques / Q000191:economics / Q000295:instrumentation*; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D013183:Sputum / Q000382:microbiology*; D013997:Time Factors; D014376:Tuberculosis / Q000175:diagnosis*