title: Morphological evidence that pentagastrin regulates secretion in the human parotid gland.
abstract: Salivary secretion is principally regulated by autonomic nerves. However, recent evidence from in vivo animal experiments suggests that gastrointestinal peptide hormones can also influence saliva production. The aim of the present study was to define the secretagogue activity of the gastrin-analogue pentagastrin in human salivary glands. For this purpose, parotid tissues were exposed to pentagastrin in vitro. Morphological techniques were used to evaluate modifications to serous acinar cells associated with secretion. Using a variant of the osmium maceration method, high resolution scanning electron microscopy allowed assessment of the morphology of the cytoplasmic aspect of the plasmalemma to demonstrate secretory activity. To quantify responses to pentagastrin, we recorded morphometric data on microvilli, microbuds, and protrusions. Dose-dependent morphological changes were observed, whereas protein concentration increased in the incubate. The use of selective receptor antagonists showed pentagastrin to act principally via cholecystokinin-A receptors. The morphological responses observed following exposure to pentagastrin differed from those elicited following exposure to the pan-muscarinic agonist carbachol. This study provides the first demonstration of a direct secretory action of gastrointestinal peptides on salivary glands in humans.
authors.affiliation: Department of Cytomorphology, University of Cagliari, Cagliari, Italy. floy@unica.it
authors.forename: Francesco
authors.initials: F
authors.lastname: Loy
authors.forename: Martina
authors.initials: M
authors.lastname: Diana
authors.forename: Raffaella
authors.initials: R
authors.lastname: Isola
authors.forename: Paola
authors.initials: P
authors.lastname: Solinas
authors.forename: Michela
authors.initials: M
authors.lastname: Isola
authors.forename: Gabriele
authors.initials: G
authors.lastname: Conti
authors.forename: Maria Serenella
authors.initials: MS
authors.lastname: Lantini
authors.forename: Margherita
authors.initials: M
authors.lastname: Cossu
authors.forename: Alessandro
authors.initials: A
authors.lastname: Riva
authors.forename: Jörgen
authors.initials: J
authors.lastname: Ekström
mesh_terms: D061354:Acinar Cells / Q000166:cytology / Q000187:drug effects; D005765:Gastrointestinal Agents / Q000494:pharmacology*; D006727:Hormone Antagonists / Q000494:pharmacology; D006801:Humans; D008854:Microscopy, Electron; D008871:Microvilli / Q000187:drug effects; D010306:Parotid Gland / Q000033:anatomy & histology / Q000187:drug effects* / Q000378:metabolism; D010418:Pentagastrin / Q000494:pharmacology*; D011377:Proglumide / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: D005765:Gastrointestinal Agents; D006727:Hormone Antagonists; D010418:Pentagastrin; D011377:Proglumide; C048181:lorglumide