title: Restriction fragment length polymorphism of the 5S-rRNA-NTS region: a rapid and precise method for plant identification.
abstract: Molecular genetic methods have several advantages over classical morphological and chemical analyses. The genetic method requires genotype instead than phenotype, therefore PCR-based techniques have been widely used for a rapid identification of plant species, varieties and chemotypes. Recently, the molecular discrimination of some higher plant species has been evaluated using sequences of a 5S-rRNA gene spacer region. The variation in the nontranscribed sequence (NTS) region has been used in a number of plant species for studying intraspecific variation, genome evolution, and phylogenetic reconstruction. Here, we describe a rapid method based on the use of the 5S-rRNA-NTS region as a tool for plant DNA fingerprinting, which combines PCR, sequencing and restriction fragment length polymorphism analyses.
authors.affiliation: Plant Physiology Unit, Department of Plant Biology, University of Turin, Turin, Italy. cinzia.bertea@unito.it
authors.forename: Cinzia Margherita
authors.initials: CM
authors.lastname: Bertea
authors.forename: Giorgio
authors.initials: G
authors.lastname: Gnavi
mesh_terms: D016172:DNA Fingerprinting; D018744:DNA, Plant / Q000737:chemistry; D004275:DNA, Ribosomal / Q000737:chemistry*; D021903:DNA, Ribosomal Spacer / Q000737:chemistry; D010944:Plants / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D018749:RNA, Plant / Q000737:chemistry*; D012341:RNA, Ribosomal, 5S / Q000737:chemistry*
chemical_list: D018744:DNA, Plant; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D018749:RNA, Plant; D012341:RNA, Ribosomal, 5S