title: Methodology for Anti-Gene Anti-IGF-I Therapy of Malignant Tumours.
abstract: The aim of this study was to establish the criteria for methodology of cellular "anti-IGF-I" therapy of malignant tumours and particularly for glioblastoma multiforme. The treatment of primary glioblastoma patients using surgery, radiotherapy, and chemotherapy was followed by subcutaneous injection of autologous cancer cells transfected by IGF-I antisense/triple helix expression vectors. The prepared cell "vaccines" should it be in the case of glioblastomas or other tumours, have shown a change of phenotype, the absence of IGF-I protein, and expression of MHC-I and B7. The peripheral blood lymphocytes, PBL cells, removed after each of two successive vaccinations, have demonstrated for all the types of tumour tested an increasing level of CD8(+) and CD8(+)28(+) molecules and a switch from CD8(+)11b(+) to CD8(+)11. All cancer patients were supervised for up to 19 months, the period corresponding to minimum survival of glioblastoma patients. The obtained results have permitted to specify the common criteria for "anti-IGF-I" strategy: characteristics sine qua non of injected "vaccines" (cloned cells IGF-I(-) and MHC-I(+)) and of PBL cells (CD8(+) increased level).
authors.affiliation: INSERM U542 and U602, Paul Brousse Hospital, Paris XI University, 16 Avenue. PV Couturier, 94807 Villejuif, France.
authors.forename: Jerzy
authors.initials: J
authors.lastname: Trojan
authors.forename: Yuexin X
authors.initials: YX
authors.lastname: Pan
authors.forename: Ming X
authors.initials: MX
authors.lastname: Wei
authors.forename: Adama
authors.initials: A
authors.lastname: Ly
authors.forename: Alexander
authors.initials: A
authors.lastname: Shevelev
authors.forename: Maciej
authors.initials: M
authors.lastname: Bierwagen
authors.forename: Marie-Yvonne
authors.initials: MY
authors.lastname: Ardourel
authors.forename: Ladislas A
authors.initials: LA
authors.lastname: Trojan
authors.forename: Alvaro
authors.initials: A
authors.lastname: Alvarez
authors.forename: Christian
authors.initials: C
authors.lastname: Andres
authors.forename: Maria C
authors.initials: MC
authors.lastname: Noguera
authors.forename: Ignacio
authors.initials: I
authors.lastname: Briceno
authors.forename: Beatriz H
authors.initials: BH
authors.lastname: Aristizabal
authors.forename: Heliodor
authors.initials: H
authors.lastname: Kasprzak
authors.forename: Huynh T
authors.initials: HT
authors.lastname: Duc
authors.forename: Donald D
authors.initials: DD
authors.lastname: Anthony