title: Depletion of CD25⁺ cells during acute toxoplasmosis does not significantly increase mortality in Swiss OF1 mice.
abstract: The interleukin (IL)-2R alpha chain (CD25) is expressed on regulatory T cells (Treg), which constitute more than 85% of the CD25+ T cell population in a naïve mouse. CD25 is also expressed on effector T cells in mice suffering from an acute infection by the obligate intracellular protozoan parasite, Toxoplasma gondii. Lethal toxoplasmosis is accompanied by a significant loss of Treg in mice naturally susceptible to toxoplasmosis. The present study was done to explore the role of Treg cells using an anti-CD25 antibody-mediated depletion in mice naturally resistant to toxoplasmosis. Although a significant decrease in the percentage of Treg cells was observed following anti-CD25 monoclonal antibody injections, the depletion of CD25+ cells during acute toxoplasmosis did not significantly increase the mortality of Swiss OF1 mice and no significant difference was observed in the brain parasitic load between the mice in the depleted-infected and isotype-infected groups. We found no significant difference between the titres of total IgG in the sera of the mice from the two groups in the chronic phase. However, CD25+ cells depletion was followed by significantly higher levels of IL-12 in the serum of depleted mice than in that of mice injected with the isotype control antibody.
authors.affiliation: Université François Rabelais de Tours, UMR1282 Infectiologie et Santé Publique, F-37000 Tours, France. drharoonakbar@yahoo.co.uk
authors.forename: Haroon
authors.initials: H
authors.lastname: Akbar
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Germon
authors.forename: Patricia
authors.initials: P
authors.lastname: Berthon
authors.forename: Isabelle
authors.initials: I
authors.lastname: Dimier-Poisson
authors.forename: Nathalie
authors.initials: N
authors.lastname: Moiré
mesh_terms: D000208:Acute Disease; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D005260:Female; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology*; D051379:Mice; D059208:Parasite Load; D050378:T-Lymphocytes, Regulatory / Q000166:cytology* / Q000276:immunology; D014122:Toxoplasma / Q000276:immunology*; D014123:Toxoplasmosis / Q000276:immunology* / Q000401:mortality / Q000473:pathology
chemical_list: D000911:Antibodies, Monoclonal; D053645:Interleukin-2 Receptor alpha Subunit