title: Protective effect of sodium ferulate on acetaldehyde-treated precision-cut rat liver slices.
abstract: Activated hepatic stellate cells (HSCs) play a key role in hepatic fibrogenesis, and inhibition of HSC activation may prevent liver fibrosis. Acetaldehyde, the most deleterious metabolite of alcohol, triggers HSC activation in alcoholic liver injury. In the present study, we investigated the protective effect of sodium ferulate (SF), a sodium salt of ferulic acid that is rich in fruits and vegetables, on acetaldehyde-stimulated HSC activation using precision-cut liver slices (PCLSs). Rat PCLSs were co-incubated with 350 μM acetaldehyde and different concentrations of SF. Hepatotoxicity was assessed by measuring enzyme leakage and malondialdehyde content in tissue. α-Smooth muscle actin, transforming growth factor-β(1), and hydroxyproline were determined to assess the activation of HSCs. In addition, matrix metalloproteinase (MMP)-1 and the tissue inhibitor of metalloproteinase (TIMP-1) were determined to evaluate collagen degradation. SF prominently prevented the enzyme leakage in acetaldehyde-treated slices and also inhibited HSC activation and collagen production stimulated by acetaldehyde. In addition, SF increased MMP-1 expression and decreased TIMP-1 expression. These results showed that SF protected PCLSs from acetaldehyde-stimulated HSC activation and liver injury, which may be associated with the attenuation of oxidative injury and acceleration of collagen degradation.
authors.affiliation: Department of Pharmacology, School of Basic Medical Science, Wuhan University, Wuhan, Hubei, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Guo
authors.forename: Xiao-Qian
authors.initials: XQ
authors.lastname: Wu
authors.forename: Chun
authors.initials: C
authors.lastname: Zhang
authors.forename: Zhang-Xiu
authors.initials: ZX
authors.lastname: Liao
authors.forename: Yong
authors.initials: Y
authors.lastname: Wu
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
mesh_terms: D000079:Acetaldehyde / Q000009:adverse effects*; D000199:Actins / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy* / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D003373:Coumaric Acids / Q000494:pharmacology / Q000627:therapeutic use*; D000431:Ethanol / Q000009:adverse effects; D055166:Hepatic Stellate Cells / Q000187:drug effects* / Q000378:metabolism; D006909:Hydroxyproline / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000378:metabolism
chemical_list: D000199:Actins; D000975:Antioxidants; D003373:Coumaric Acids; D010936:Plant Extracts; D019715:Tissue Inhibitor of Metalloproteinase-1; D016212:Transforming Growth Factor beta; D000431:Ethanol; D008315:Malondialdehyde; D003094:Collagen; C004999:ferulic acid; D020781:Matrix Metalloproteinase 1; D000079:Acetaldehyde; D006909:Hydroxyproline