title: Combination of enzymatic hydrolysis and ethanol organosolv pretreatments: effect on lignin structures, delignification yields and cellulose-to-glucose conversion.
abstract: Enzymatic pre-hydrolysis using the industrial enzymatic cocktail Cellulyve® was assessed as a first step in a pretreatment process of Miscanthus biomass involving an aqueous-ethanol organosolv treatment. (13)C and (31)P Nuclear Magnetic Resonance and size exclusion chromatography were used to analyze the cellulose and lignin before and after treatment. It was demonstrated that despite a very low impact on the fibre structure (observed by Scanning Electron Microscopy) and composition (in terms of sugars and polyphenolics content), the enzymatic pre-treatment disrupted the lignocellulosic matrix to a considerable extend. This weakening permitted enhanced removal of lignin during organosolv pulping and increased hydrolysability of the residual cellulosic pulp for the production of monomeric glucose. Using this combined treatment, a delignification yield of 93% and an enzymatic cellulose-to-glucose conversion of 75% were obtained.
authors.affiliation: Laboratoire d'Etude et de Recherche sur le MAteriau Bois, Faculté des Sciences et Techniques, Université de Lorraine, Bld des Aiguillettes, BP 70236, F-54506 Vandoeuvre-lès-Nancy cedex, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Obama
authors.forename: Guillaume
authors.initials: G
authors.lastname: Ricochon
authors.forename: Lionel
authors.initials: L
authors.lastname: Muniglia
authors.forename: Nicolas
authors.initials: N
authors.lastname: Brosse
mesh_terms: D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism*; D000431:Ethanol / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism*; D006868:Hydrolysis / Q000187:drug effects; D008031:Lignin / Q000737:chemistry* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D006109:Poaceae / Q000187:drug effects; D012995:Solubility / Q000187:drug effects; D012997:Solvents / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D012997:Solvents; D000431:Ethanol; D002482:Cellulose; D008031:Lignin; D002480:Cellulase; D005947:Glucose