title: [Human nerve stem cells in vitro].
abstract: The expansion of Nest-positive nerve stem cells fraction in the culture of human fetal brain was investigated in this work. Our results demonstrated the valuable increase of the percentage of Nest-positive cells from 44.1 to 62.5% of cell population at the response to the presence of EGF and bFGF and using the heat-inactivated serum in the culture medium. Further differentiation in culture tended to decrease of Nest-positive cell amount. Stimulation of the neuroinduction with retinoic acid resulted in decrease of the percentage of these cells in the population to 14.1%. This data allowed obtaining the nerve cell population more effective for using as a graft material in cell therapy.
authors.forename: V I
authors.initials: VI
authors.lastname: Tsymbaliuk
authors.forename: I H
authors.initials: IH
authors.lastname: Vasil'eva
authors.forename: N P
authors.initials: NP
authors.lastname: Oleksenko
authors.forename: N H
authors.initials: NH
authors.lastname: Chopyk
authors.forename: O I
authors.initials: OI
authors.lastname: Tsiubko
authors.forename: O S
authors.initials: OS
authors.lastname: Halanta
authors.forename: N D
authors.initials: ND
authors.lastname: Snitsar
mesh_terms: D001921:Brain / Q000166:cytology* / Q000187:drug effects / Q000196:embryology; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media; D005333:Fetus; D006801:Humans; D058953:Neural Stem Cells / Q000166:cytology* / Q000187:drug effects; D014212:Tretinoin / Q000494:pharmacology
chemical_list: D003470:Culture Media; D014212:Tretinoin