title: Peroxiredoxins 3 and 4 are overexpressed in prostate cancer tissue and affect the proliferation of prostate cancer cells in vitro.
abstract: The present study aimed to investigate the proteome profiling of surgically treated prostate cancers. Hereto, 2D-DIGE and mass spectrometry were performed for protein identification, and data validation for peroxiredoxin 3 and 4 (PRDX3 and PRDX4) was accomplished by reverse phase protein arrays (RPPA). The Formal Concept Analysis (FCA) method was applied to assess whether the TMPRSS2-ERG gene fusion could influence the degree of overexpression of PRDX3 and PRDX4 in prostate cancer. Lastly, we performed an in vitro functional characterization of both PRDX3 and PRDX4 using the classical human prostate cancer cell lines DU145 and LNCaP. Reverse phase protein arrays verified that the overexpression of both PRDX3 and PRDX4 in tumor samples is negatively correlated with the presence of the TMPRSS2-ERG gene fusion. Functional characterization of PRDX3 and PRDX4 activity in PCa cell lines suggests a role of these members of the peroxiredoxin family in the pathophysiology of this tumor entity.
authors.affiliation: Department of Oncology, Haematology and Bone marrow transplantation, section Pneumology, Hubertus Wald-Tumour Zentrum, University Hospital Eppendorf, Hamburg, Germany.
authors.forename: Ramesh
authors.initials: R
authors.lastname: Ummanni
authors.forename: Frederico
authors.initials: F
authors.lastname: Barreto
authors.forename: Simone
authors.initials: S
authors.lastname: Venz
authors.forename: Christian
authors.initials: C
authors.lastname: Scharf
authors.forename: Christine
authors.initials: C
authors.lastname: Barett
authors.forename: Heiko A
authors.initials: HA
authors.lastname: Mannsperger
authors.forename: Jan Christoph
authors.initials: JC
authors.lastname: Brase
authors.forename: Ruprecht
authors.initials: R
authors.lastname: Kuner
authors.forename: Thorsten
authors.initials: T
authors.lastname: Schlomm
authors.forename: Guido
authors.initials: G
authors.lastname: Sauter
authors.forename: Holger
authors.initials: H
authors.lastname: Sültmann
authors.forename: Ulrike
authors.initials: U
authors.lastname: Korf
authors.forename: Carsten
authors.initials: C
authors.lastname: Bokemeyer
authors.forename: Reinhard
authors.initials: R
authors.lastname: Walther
authors.forename: Tim H
authors.initials: TH
authors.lastname: Brümmendorf
authors.forename: Stefan
authors.initials: S
authors.lastname: Balabanov
mesh_terms: D048708:Cell Growth Processes / Q000502:physiology; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D050939:Gene Fusion; D006801:Humans; D008297:Male; D061105:Peroxiredoxin III / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D054464:Peroxiredoxins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D011467:Prostate / Q000737:chemistry / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D020543:Proteome / Q000032:analysis; D040901:Proteomics
chemical_list: D020543:Proteome; C516115:PRDX3 protein, human; C515980:PRDX4 protein, human; D061105:Peroxiredoxin III; D054464:Peroxiredoxins