title: Insights into the regulation of protein abundance from proteomic and transcriptomic analyses.
abstract: Recent advances in next-generation DNA sequencing and proteomics provide an unprecedented ability to survey mRNA and protein abundances. Such proteome-wide surveys are illuminating the extent to which different aspects of gene expression help to regulate cellular protein abundances. Current data demonstrate a substantial role for regulatory processes occurring after mRNA is made - that is, post-transcriptional, translational and protein degradation regulation - in controlling steady-state protein abundances. Intriguing observations are also emerging in relation to cells following perturbation, single-cell studies and the apparent evolutionary conservation of protein and mRNA abundances. Here, we summarize current understanding of the major factors regulating protein expression.
authors.affiliation: Center for Genomics and Systems Biology, New York University, New York 10003, USA. cvogel@nyu.edu
authors.forename: Christine
authors.initials: C
authors.lastname: Vogel
authors.forename: Edward M
authors.initials: EM
authors.lastname: Marcotte
mesh_terms: D020869:Gene Expression Profiling; D011506:Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism*
chemical_list: D011506:Proteins; D020543:Proteome; D012333:RNA, Messenger