title: DyeCycle Violet used for side population detection is a substrate of P-glycoprotein.
abstract: Cancer stem cells (CSC) are increasingly recognized as key target cells for cancer therapy because they are both tumorigenic and chemoresistant and, therefore, can give rise to recurrent disease. Side population (SP) sorting using an ultraviolet (UV) laser is an established method to isolate CSC based on ABC drug transporter-dependent (e.g., ABCG2) Hoechst 33342 efflux. We here show that Vybrant® DyeCycle™ Violet (DCV), a DNA-binding fluorophor allowing SP sorting using a non-UV laser (such as violet laser), is transported via P-glycoprotein (PgP). Because PgP might be particularly abundant in multidrug-resistant cancer cells rather than bona fide CSC, investigators using DCV should be aware that this strategy might also detect PgP-expressing non-CSC.
authors.affiliation: Internal Medicine V, Tyrolean Cancer Research Institute, Innsbruck Medical University, Innsbruck, Austria.
authors.forename: Maximilian
authors.initials: M
authors.lastname: Boesch
authors.forename: Daniel
authors.initials: D
authors.lastname: Reimer
authors.forename: Holger
authors.initials: H
authors.lastname: Rumpold
authors.forename: Alain G
authors.initials: AG
authors.lastname: Zeimet
authors.forename: Sieghart
authors.initials: S
authors.lastname: Sopper
authors.forename: Dominik
authors.initials: D
authors.lastname: Wolf
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000032:analysis* / Q000378:metabolism; D001562:Benzimidazoles; D001692:Biological Transport; D002469:Cell Separation / Q000379:methods*; D005189:False Positive Reactions; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D006801:Humans; D020014:K562 Cells; D007834:Lasers; D009363:Neoplasm Proteins / Q000032:analysis* / Q000378:metabolism; D014411:Neoplastic Stem Cells / Q000378:metabolism* / Q000473:pathology; D014466:Ultraviolet Rays
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D001562:Benzimidazoles; D005456:Fluorescent Dyes; D009363:Neoplasm Proteins; C017807:bisbenzimide ethoxide trihydrochloride