title: Association between single nucleotide polymorphisms in p53 and abortion in Thoroughbred mares.
abstract: Single nucleotide polymorphisms (SNPs) in the p53 gene have been studied extensively in humans. The aims of this study were to determine the frequency of the Arg/Pro SNP in p53 in Thoroughbred mares on one stud in Brazil and to correlate p53 genotypes with reproductive performance. SNPs were detected by PCR-restriction fragment length polymorphism in blood samples from 105 horses and confirmed by sequencing. The allele frequency in Thoroughbred mares at codon 72 in exon 4 was 73.3% Arg/Pro, 17.1% Arg/Arg and 9.6% Pro/Pro. The presence of Arg/Pro was significantly associated with abortion (P=0.02), while Pro/Pro mares had a lower probability of abortion (P<0.05). Using a logistic regression model, the dominance effect was significant (P=0.044; odds ratio 7.94) for abortion and additive effects were not significant (P=0.26). p53 may play a role in equine reproduction.
authors.affiliation: Laboratório de Embriologia Molecular e Transgênese, Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
authors.forename: Priscila Marques Moura de
authors.initials: PM
authors.lastname: Leon
authors.forename: Vinicus Farias
authors.initials: VF
authors.lastname: Campos
authors.forename: Helena Strelow
authors.initials: HS
authors.lastname: Thurow
authors.forename: Fernando Pires
authors.initials: FP
authors.lastname: Hartwig
authors.forename: Lisiane Priscila
authors.initials: LP
authors.lastname: Selau
authors.forename: Odir Antonio
authors.initials: OA
authors.lastname: Dellagostin
authors.forename: José Braccini
authors.initials: JB
authors.lastname: Neto
authors.forename: João Carlos
authors.initials: JC
authors.lastname: Deschamps
authors.forename: Fabiana Kömmling
authors.initials: FK
authors.lastname: Seixas
authors.forename: Tiago
authors.initials: T
authors.lastname: Collares
mesh_terms: D000034:Abortion, Veterinary / Q000453:epidemiology / Q000209:etiology / Q000235:genetics*; D000818:Animals; D001120:Arginine / Q000235:genetics; D001938:Brazil / Q000453:epidemiology; D003062:Codon; D005091:Exons; D005260:Female; D005787:Gene Frequency; D016158:Genes, p53; D056726:Genetic Association Studies / Q000662:veterinary; D005838:Genotype; D006734:Horse Diseases / Q000453:epidemiology / Q000209:etiology / Q000235:genetics*; D006736:Horses; D016015:Logistic Models; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D011392:Proline / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000662:veterinary
chemical_list: D003062:Codon; D001120:Arginine; D011392:Proline