title: Steroids from the soft coral Sinularia crassa.
abstract: One new sterol, crassarosterol A (1), and four new steroidal glycosides, crassarosterosides A-D (2-5) were isolated from the Formosan soft coral Sinularia crassa. The absolute configuration of 1 was determined using the Mosher's method. The absolute configurations for the sugar moieties of 2-5 were determined by HPLC analysis on the o-tolylthiocarbamates derived from the liberated sugar after acid hydrolysis. Compounds 2 and 4 could significantly inhibit the expression of pro-inflammatory iNOS protein at 10 µM. In contrast, 1-3 were found to stimulate the expression of COX-2 protein at this concentration. Steroids 1 and 4 also showed cytotoxicity toward the selected human liver cancer cells.
authors.affiliation: Chinese Medicinal Research and Development Center, China Medical University Hospital, Taichung 40402, Taiwan.|China Medical University, Taichung, 40402, Taiwan.
authors.forename: Chih-Hua
authors.initials: CH
authors.lastname: Chao
authors.affiliation: Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 80424, Taiwan.
authors.forename: Kuei-Ju
authors.initials: KJ
authors.lastname: Chou
authors.affiliation: Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 80424, Taiwan.
authors.forename: Chiung-Yao
authors.initials: CY
authors.lastname: Huang
authors.affiliation: Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 80424, Taiwan.|Asian Pacific Ocean Research Center, National Sun Yat-sen University, Kaohsiung 80424, Taiwan.
authors.forename: Zhi-Hong
authors.identifier: 0000-0003-4411-044X
authors.initials: ZH
authors.lastname: Wen
authors.affiliation: Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 80424, Taiwan.|Asian Pacific Ocean Research Center, National Sun Yat-sen University, Kaohsiung 80424, Taiwan.
authors.forename: Chi-Hsin
authors.initials: CH
authors.lastname: Hsu
authors.affiliation: Graduate Institute of Integrated Medicine, College of Chinese Medicine, China Medical University, Taichung 40402, Taiwan.
authors.forename: Yang-Chang
authors.initials: YC
authors.lastname: Wu
authors.affiliation: Institute of Oceanography, National Taiwan University, Taipei, 10617, Taiwan.
authors.forename: Chang-Feng
authors.initials: CF
authors.lastname: Dai
authors.affiliation: Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 80424, Taiwan.|Asian Pacific Ocean Research Center, National Sun Yat-sen University, Kaohsiung 80424, Taiwan.
authors.forename: Jyh-Horng
authors.initials: JH
authors.lastname: Sheu
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000378:metabolism*; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D051546:Cyclooxygenase 2 / Q000378:metabolism; D055808:Drug Discovery; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D020128:Inhibitory Concentration 50; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D051379:Mice; D015394:Molecular Structure; D009369:Neoplasms / Q000188:drug therapy; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010140:Pacific Ocean; D013237:Stereoisomerism; D013261:Sterols / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D013624:Taiwan
chemical_list: D000893:Anti-Inflammatory Agents; D000970:Antineoplastic Agents; D006027:Glycosides; D013261:Sterols; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2