title: Fluorescence imaging and targeted distribution of bacterial magnetic particles in nude mice.
abstract: Bacterial magnetic particles (BMPs) are of interest as potential carriers of bioactive macromolecules, drugs, or liposomes. In this study, a high-pressure homogenizer was used to disrupt Magnetospirillum gryphiswaldense strain MSR-1 cells, and BMPs were purified. BMPs were labeled with fluorescence reagent 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocianin perchlorate (DiI) and injected into the tail vein of BALB/c nude mice. Distribution of fluorescence signals of DiI-BMPs in vivo was examined using a whole-body fluorescence imaging system. The result showed that fluorescence signals were detected in liver, stomach, intestine, lungs, and spleen. However, transmission electron microscopy of ultrathin sections indicated that BMPs were mainly present in liver and lungs, but not in the other organs. BMPs could be useful as carriers for targeted drug therapy of diseases of the liver or lung.
authors.affiliation: College of Biological Sciences, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Tao
authors.initials: T
authors.lastname: Tang
authors.forename: Lianfeng
authors.initials: L
authors.lastname: Zhang
authors.forename: Ran
authors.initials: R
authors.lastname: Gao
authors.forename: Yunping
authors.initials: Y
authors.lastname: Dai
authors.forename: Fanchao
authors.initials: F
authors.lastname: Meng
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
mesh_terms: D000825:Animal Structures / Q000378:metabolism / Q000648:ultrastructure; D000818:Animals; D002232:Carbocyanines / Q000378:metabolism; D004337:Drug Carriers / Q000008:administration & dosage* / Q000737:chemistry* / Q000493:pharmacokinetics; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000378:metabolism; D055944:Magnetosomes / Q000378:metabolism*; D042201:Magnetospirillum / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry*; D013194:Staining and Labeling / Q000379:methods; D051598:Whole Body Imaging
chemical_list: C035005:3,3'-dihexadecylindocarbocyanine; D002232:Carbocyanines; D004337:Drug Carriers; D005456:Fluorescent Dyes