title: Micro-pattern guided adhesion of osteoblasts on diamond surfaces.
abstract: Microscopic chemical patterning of diamond surfaces by hydrogen and oxygen surface atoms is used for self-assembly of human osteoblastic cells into micro-arrays. The cell adhesion and assembly is further controlled by concentration of cells (2,500-10,000 cells/cm(2)) and fetal bovine serum (0-15%). The cells are characterized by fluorescence microscopy of actin fibers and nuclei. The serum protein adsorption is studied by atomic force microscopy (AFM). The cells are arranged selectively on O-terminated patterns into 30-200 μm wide arrays. Higher cell concentrations allow colonization of unfavorable H-terminated regions due to mutual cell communication. There is no cell selectivity without the proteins in the medium. Based on the AFM, the proteins are present on both H- and O-terminated surfaces. Pronounced differences in their thickness, surface roughness, morphology, and phase images indicate different conformation of the proteins and explain the cell selectivity.
authors.affiliation: Institute of Physics, Academy of Sciences of the Czech Republic, Cukrovarnicka 10, Prague 6, Czech Republic; E-Mail:  ukraintsev@fzu.cz ;  kromka@fzu.cz.
authors.forename: Bohuslav
authors.initials: B
authors.lastname: Rezek
authors.forename: Lenka
authors.initials: L
authors.lastname: Michalíková
authors.forename: Egor
authors.initials: E
authors.lastname: Ukraintsev
authors.forename: Alexander
authors.initials: A
authors.lastname: Kromka
authors.forename: Marie
authors.initials: M
authors.lastname: Kalbacova