title: Antibacterial nanocomposite with calcium phosphate and quaternary ammonium.
abstract: Secondary caries is a frequent reason for restoration failure, resulting from acidogenic bacteria and their biofilms. The objectives of this study were to: (1) develop a novel nanocomposite containing nanoparticles of amorphous calcium phosphate (NACP) and quaternary ammonium dimethacrylate (QADM); and (2) investigate its mechanical and antibacterial durability. A spray-drying technique yielded NACP with particle size of 116 nm. The nanocomposite contained NACP and reinforcement glass fillers, with QADM in the resin. Two commercial composites were tested as controls. Composites were inoculated with Streptococcus mutans. After 180-day water-aging, NACP+QADM nanocomposite had flexural strength and elastic modulus matching those of commercial controls (p > 0.1). NACP+QADM nanocomposite reduced the biofilm colony-forming units (CFU) by 3-fold, compared with commercial composites (p < 0.05). Metabolic activity and lactic acid production of biofilms on NACP+QADM were much less than those on commercial composites (p < 0.05). The antibacterial properties of NACP+QADM were maintained after water-aging for 30, 90, and 180 d (p > 0.05). In conclusion, the novel NACP-QADM nanocomposite greatly decreased biofilm metabolic activity, CFU, and lactic acid, while matching the load-bearing capability of commercial composites without antibacterial properties. The NACP-QADM nanocomposite with strong and durable antibacterial properties, together with its previously reported Ca-PO(4) release capability, may render it useful for caries-inhibiting restorations.
authors.affiliation: Biomaterials & Tissue Engineering Division, Dept. of Endodontics, Prosthodontics and Operative Dentistry, University of Maryland Dental School, Baltimore, MD 21201, USA.
authors.forename: L
authors.initials: L
authors.lastname: Cheng
authors.forename: M D
authors.initials: MD
authors.lastname: Weir
authors.forename: K
authors.initials: K
authors.lastname: Zhang
authors.forename: S M
authors.initials: SM
authors.lastname: Xu
authors.forename: Q
authors.initials: Q
authors.lastname: Chen
authors.forename: X
authors.initials: X
authors.lastname: Zhou
authors.forename: H H K
authors.initials: HH
authors.lastname: Xu
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects; D002130:Calcium Phosphates / Q000494:pharmacology*; D002327:Cariostatic Agents / Q000737:chemistry* / Q000494:pharmacology; D015169:Colony Count, Microbial; D003188:Composite Resins / Q000737:chemistry* / Q000494:pharmacology; D003731:Dental Caries / Q000517:prevention & control; D003793:Dental Restoration, Permanent / Q000379:methods*; D003799:Dental Stress Analysis; D055119:Elastic Modulus; D019344:Lactic Acid / Q000378:metabolism; D008422:Materials Testing; D053761:Nanocomposites / Q000737:chemistry*; D018583:Pliability; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*; D013295:Streptococcus mutans / Q000187:drug effects* / Q000378:metabolism; D014867:Water
chemical_list: D000900:Anti-Bacterial Agents; D002130:Calcium Phosphates; D002327:Cariostatic Agents; D003188:Composite Resins; D000644:Quaternary Ammonium Compounds; C519480:amorphous calcium phosphate; D014867:Water; D019344:Lactic Acid