title: Microchip electrophoresis with amperometric detection for the study of the generation of nitric oxide by NONOate salts.
abstract: Microchip electrophoresis (ME) with electrochemical detection was used to monitor nitric oxide (NO) production from diethylammonium (Z)-1-(N,N-diethylamino)diazen-1-ium-1,2-diolate (DEA/NO) and 1-(hydroxyl-NNO-azoxy)-L-proline disodium salt (PROLI/NO). NO was generated through acid hydrolysis of these NONOate salts. The products of acid hydrolysis were introduced into a 5-cm separation channel using gated injection. The separation was accomplished using reverse polarity and a background electrolyte consisting of 10 mM boric acid and 2 mM tetradecyltrimethylammonium bromide, pH 11. Electrochemical detection was performed using an isolated potentiostat in an in-channel configuration. Potentials applied to the working electrode, typically higher than +1.0 V vs. Ag/AgCl, allowed the direct detection of nitrite, NO, DEA/NO, and PROLI/NO. Baseline resolution was achieved for the separation of PROLI/NO and NO while resolution between DEA/NO and NO was poor (1.0 ± 0.2). Nitrite was present in all samples tested.
authors.affiliation: Department of Chemistry, University of Kansas, Lawrence, KS 66045, USA.
authors.forename: Dulan B
authors.initials: DB
authors.lastname: Gunasekara
authors.forename: Matthew K
authors.initials: MK
authors.lastname: Hulvey
authors.forename: Susan M
authors.initials: SM
authors.lastname: Lunte
authors.forename: José Alberto Fracassi
authors.initials: JA
authors.lastname: da Silva
mesh_terms: D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods; D046209:Electrophoresis, Microchip / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D006834:Hydrazines / Q000494:pharmacology*; D006868:Hydrolysis; D009569:Nitric Oxide / Q000032:analysis* / Q000378:metabolism; D020030:Nitric Oxide Donors / Q000494:pharmacology*; D009573:Nitrites / Q000032:analysis; D011392:Proline / Q000031:analogs & derivatives* / Q000494:pharmacology; D012680:Sensitivity and Specificity
chemical_list: D006834:Hydrazines; D020030:Nitric Oxide Donors; D009573:Nitrites; C102043:proline-nitric oxide; D009569:Nitric Oxide; C084012:1,1-diethyl-2-hydroxy-2-nitrosohydrazine; D011392:Proline