title: Isolation of potentially novel Brucella spp. from frogs.
abstract: Bacterial isolates from frogs were phenotypically identified as Ochrobactrum anthropi, but 16S rRNA sequencing showed up to 100% identity with Brucella inopinata. Further analysis of recA, omp2a, omp2b, bcsp31, and IS711 and multilocus sequence analysis (MLSA) verified a close relationship with Brucella, suggesting the isolates may actually represent novel members of this growing genus of zoonotic pathogens.
authors.affiliation: Landesbetrieb Hessisches Landeslabor (LHL), Abteilung Veterinärmedizin, Gießen, Germany. tobias.eisenberg@lhl.hessen.de
authors.forename: Tobias
authors.initials: T
authors.lastname: Eisenberg
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Hamann
authors.forename: Ute
authors.initials: U
authors.lastname: Kaim
authors.forename: Karen
authors.initials: K
authors.lastname: Schlez
authors.forename: Helga
authors.initials: H
authors.lastname: Seeger
authors.forename: Nicole
authors.initials: N
authors.lastname: Schauerte
authors.forename: Falk
authors.initials: F
authors.lastname: Melzer
authors.forename: Herbert
authors.initials: H
authors.lastname: Tomaso
authors.forename: Holger C
authors.initials: HC
authors.lastname: Scholz
authors.forename: Mark S
authors.initials: MS
authors.lastname: Koylass
authors.forename: Adrian M
authors.initials: AM
authors.lastname: Whatmore
authors.forename: Michael
authors.initials: M
authors.lastname: Zschöck
mesh_terms: D000818:Animals; D001001:Anura / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D002002:Brucella / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S