title: In vitro antibacterial efficacy of cetylpyridinium chloride-containing mouthwashes.
abstract: OBJECTIVE
  The objective of this study was to examine the ability of three CPC-containing mouthwashes to kill planktonic bacteria in an in vitro short-exposure assay.
  
  
  METHODS
  This blind study was conducted on two common oral bacterial species: Aggregatibacter (Actinobacillus) actinomycetemcomitans and Streptococcus mutans. The following mouthwashes were tested: two containing 0.075% CPC and 0.05% NaF in an alcohol-free base, and one containing 0.075% CPC and 0.05% NaF plus 6% alcohol. Additionally, a 0.05% NaF-only mouthwash was included as a negative control. Bacteria were exposed to one of the test mouthwashes for 30 seconds and then washed thoroughly, serially diluted, and plated on appropriate media to determine viable bacterial counts. Viable counts were converted to a log reduction in colony forming units (CFUs) relative to the negative control.
  
  
  RESULTS
  All three test mouthwashes included in this study gave a statistically significant reduction of > 3 log CFUs relative to samples treated with the negative control.
  
  
  CONCLUSIONS
  All three experimental 0.075% CPC mouthwash formulas gave a > 99.9% reduction in viable bacteria of both species following 30 seconds of treatment.
authors.affiliation: Colgate-Palmolive Technology Center Piscataway, NJ, USA. Lyndsay_Schaeffer-Korbylo@colpal.com
authors.forename: Lyndsay M
authors.initials: LM
authors.lastname: Schaeffer
authors.forename: Gregory
authors.initials: G
authors.lastname: Szewczyk
authors.forename: Jason
authors.initials: J
authors.lastname: Nesta
authors.forename: Mark
authors.initials: M
authors.lastname: Vandeven
authors.forename: Laurence
authors.initials: L
authors.lastname: Du-Thumm
authors.forename: Malcolm I
authors.initials: MI
authors.lastname: Williams
authors.forename: Evangelia
authors.initials: E
authors.lastname: Arvanitidou
mesh_terms: D016976:Aggregatibacter actinomycetemcomitans / Q000187:drug effects; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000494:pharmacology*; D000891:Anti-Infective Agents, Local / Q000008:administration & dosage / Q000494:pharmacology*; D058491:Bacterial Load / Q000187:drug effects; D002327:Cariostatic Agents / Q000494:pharmacology; D002594:Cetylpyridinium / Q000008:administration & dosage / Q000494:pharmacology*; D000431:Ethanol; D006801:Humans; D008422:Materials Testing; D050296:Microbial Viability / Q000187:drug effects; D009067:Mouthwashes / Q000494:pharmacology*; D014677:Pharmaceutical Vehicles; D016037:Single-Blind Method; D012969:Sodium Fluoride / Q000494:pharmacology; D013295:Streptococcus mutans / Q000187:drug effects; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D000891:Anti-Infective Agents, Local; D002327:Cariostatic Agents; D009067:Mouthwashes; D014677:Pharmaceutical Vehicles; D000431:Ethanol; D012969:Sodium Fluoride; D002594:Cetylpyridinium