title: Carbon sources and medium pH affects the growth of Withania somnifera (L.) Dunal adventitious roots and withanolide A production.
abstract: This work deals with optimisation of adventitious root suspension culture of Withania somnifera (L.) Dunal (Solanaceae) for the production of biomass and withanolide A and we investigated the effect of carbon source and the initial medium pH on growth and production of withanolide A in adventitious root cultures of Withania somnifera. A 2% sucrose concentration was found to be best for both biomass (113.58 g L(-1) fresh weight (FW) and 11.33 g L(-1) dry weight (DW)) and secondary metabolite accumulation (8.93 mg g(-1) DW) in the tested range of concentration (1-8%). The biomass of adventitious roots was optimal when the initial medium pH was 5.8 (113.26 g L(-1) FW and 11.33 g L(-1) DW) but the withanolide A production was highest at the medium pH level of 5.5 (9.09 mg g(-1) DW).
authors.affiliation: Plant Biotechnology Laboratory, Department of Botany, Karnatak University, Dharwad 580003, Karnataka, India. nmurthy60@yahoo.co.in
authors.forename: H N
authors.initials: HN
authors.lastname: Murthy
authors.forename: N
authors.initials: N
authors.lastname: Praveen
mesh_terms: D018533:Biomass; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006863:Hydrogen-Ion Concentration; D010936:Plant Extracts / Q000302:isolation & purification / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects* / Q000254:growth & development*; D013395:Sucrose / Q000494:pharmacology; D032341:Withania / Q000254:growth & development / Q000378:metabolism*; D054358:Withanolides / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: C433551:3-rhamnopyranosyl(1-4)-glucopyranosyl-12-diacetoxy-20-hydroxywitha-5,24-dienolide; D003470:Culture Media; D010936:Plant Extracts; D054358:Withanolides; D013395:Sucrose