title: Temperature dependent N-glycosylation of plasma membrane heat shock protein Hsp30p in Saccharomyces cerevisiae.
abstract: The HSP30 gene of the budding yeast Saccharomyces cerevisiae encodes a seven-transmembrane heat shock protein expressed in response to various types of stress including heat shock. Although Hsp30p contains a potential N-glycosylation consensus sequence (Asn(2)-Asp(3)-Thr(4)), whether it is actually N-glycosylated has not been verified. Here we demonstrate that N-glycosylation is induced at Asn(2) of Hsp30p by severe heat shock, ethanol stress, and acetic acid stress. Mild heat shock and glucose depletion induced the expression but not N-glycosylation of Hsp30p, indicating the N-glycosylation to be dependent on temperature and environmental conditions. N-glycosylation did not affect on the intracellular localization of Hsp30p but its physiological role under severe heat shock conditions. Since limited information is available on stress-responsive or condition-induced N-glycosylation, our findings provide new insight into the regulation of cellular stress response in yeast.
authors.affiliation: Laboratory of Molecular Microbiology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan.
authors.forename: Ken'ichi
authors.initials: K
authors.lastname: Kamo
authors.forename: Akiko
authors.initials: A
authors.lastname: Takabatake
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Inoue
authors.forename: Shingo
authors.initials: S
authors.lastname: Izawa
mesh_terms: D019342:Acetic Acid / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism*; D000431:Ethanol / Q000494:pharmacology; D006031:Glycosylation; D050890:HSP30 Heat-Shock Proteins / Q000378:metabolism*; D018869:Heat-Shock Response; D006358:Hot Temperature; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*
chemical_list: D050890:HSP30 Heat-Shock Proteins; C494390:HSP30 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D000431:Ethanol; D019342:Acetic Acid