title: [Establishment of the rapid, hypersensitive testing systems for sepsis/SIRS].
abstract: We developed a new infectious disease diagnostic system in order to raise the lifesaving rate in systemic inflammatory reactive syndrome, SIRS. Using eukaryote-derived thermostable DNA polymerase, the sensitive and reliable detection of bacteria becomes feasible for large fields, thereby making the development of a wide range of powerful applications possible. Further, we have established a novel measurement system for NF-kappaB activity using fluorescence correlation spectroscopy(FCS) because the elevation of NF-kappaB activity reflects acute inflammation. NF-kappaB activity measurement in patients with SIRS may provide a useful inflammatory marker.
authors.affiliation: Department of Clinical Laboratory and Molecular Pathology, Graduate School of Medical and Pharmaceutical Science, University of Toyama, Toyama 930-0194, Japan. kitajima@med.u-toyama.ac.jp
authors.forename: Isao
authors.initials: I
authors.lastname: Kitajima
authors.forename: Hideki
authors.initials: H
authors.lastname: Niimi
mesh_terms: D015415:Biomarkers / Q000097:blood; D004259:DNA-Directed DNA Polymerase; D056890:Eukaryota / Q000201:enzymology; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D016328:NF-kappa B / Q000097:blood*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods; D018746:Systemic Inflammatory Response Syndrome / Q000175:diagnosis*
chemical_list: D015415:Biomarkers; D016328:NF-kappa B; D004259:DNA-Directed DNA Polymerase