title: A clinical study of alveolar bone tissue engineering with cultured autogenous periosteal cells: coordinated activation of bone formation and resorption.
abstract: In ongoing clinical research into the use of cultured autogenous periosteal cells (CAPCs) in alveolar bone regeneration, CAPCs were grafted into 33 sites (15 for alveolar ridge augmentation and 18 for maxillary sinus lift) in 25 cases. CAPCs were cultured for 6weeks, mixed with particulate autogenous bone and platelet-rich plasma, and then grafted into the sites. Clinical outcomes were determined from high-resolution three-dimensional computed tomography (3D-CT) images and histological findings. No serious adverse events were attributable to the use of grafted CAPCs. Bone regeneration was satisfactory even in cases of advanced atrophy of the alveolar process. Bone biopsy after bone grafting with CAPCs revealed prominent recruitment of osteoblasts and osteoclasts accompanied by angiogenesis around the regenerated bone. 3D-CT imaging suggested that remodeling of the grafted autogenous cortical bone particles was faster in bone grafting with CAPCs than in conventional bone grafting. The use of CAPCs offers cell-based bone regeneration therapy, affording complex bone regeneration across a wide area, and thus expanding the indications for dental implants. Also, it enables the content of particulate autogenous bone in the graft material to be reduced to as low as 40%, making the procedure less invasive, or enabling larger amounts of graft materials to be prepared. It may also be possible to dispense with the use of autogenous bone altogether in the future. The results suggest that CAPC grafting induces bone remodeling, thereby enhancing osseointegration and consequently reducing postoperative waiting time after dental implant placement.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Niigata University Graduate School of Medical and Dental Sciences, 2-5274 Gakkocho-dori, Chuo-ku, Niigata 951-8514, Japan. nagata@dent.niigata-u.ac.jp
authors.forename: Masaki
authors.initials: M
authors.lastname: Nagata
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Hoshina
authors.forename: Minqi
authors.initials: M
authors.lastname: Li
authors.forename: Megumi
authors.initials: M
authors.lastname: Arasawa
authors.forename: Kohya
authors.initials: K
authors.lastname: Uematsu
authors.forename: Shin
authors.initials: S
authors.lastname: Ogawa
authors.forename: Kazuho
authors.initials: K
authors.lastname: Yamada
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Kawase
authors.forename: Kenji
authors.initials: K
authors.lastname: Suzuki
authors.forename: Akira
authors.initials: A
authors.lastname: Ogose
authors.forename: Ichiro
authors.initials: I
authors.lastname: Fuse
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Okuda
authors.forename: Katsumi
authors.initials: K
authors.lastname: Uoshima
authors.forename: Koh
authors.initials: K
authors.lastname: Nakata
authors.forename: Hiromasa
authors.initials: H
authors.lastname: Yoshie
authors.forename: Ritsuo
authors.initials: R
authors.lastname: Takagi
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000293:Adolescent; D000368:Aged; D000540:Alveolar Ridge Augmentation / Q000379:methods*; D001706:Biopsy; D001861:Bone Regeneration; D001862:Bone Resorption / Q000473:pathology* / Q000503:physiopathology; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D008297:Male; D008334:Mandible / Q000000981:diagnostic imaging / Q000201:enzymology / Q000473:pathology / Q000601:surgery*; D008443:Maxillary Sinus / Q000000981:diagnostic imaging / Q000473:pathology / Q000503:physiopathology / Q000601:surgery; D008875:Middle Aged; D010012:Osteogenesis; D010521:Periosteum / Q000166:cytology* / Q000637:transplantation*; D000071681:Tartrate-Resistant Acid Phosphatase; D023822:Tissue Engineering / Q000379:methods*; D014057:Tomography, X-Ray Computed; D014182:Transplantation, Autologous
chemical_list: D007527:Isoenzymes; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase