title: Insights into substrate recognition by the Escherichia coli Orf135 protein through its solution structure.
abstract: Escherichia coli Orf135 hydrolyzes oxidatively damaged nucleotides such as 2-hydroxy-dATP, 8-oxo-dGTP and 5-hydroxy-CTP, in addition to 5-methyl-dCTP, dCTP and CTP. Nucleotide pool sanitization by Orf135 is important since nucleotides are continually subjected to potential damage by reactive oxygen species produced during respiration. Orf135 is a member of the Nudix family of proteins which hydrolyze nucleoside diphosphate derivatives. Nudix hydrolases are characterized by the presence of a conserved motif, even though they recognize various substrates and possess a variety of substrate binding pockets. We investigated the tertiary structure of Orf135 and its interaction with a 2-hydroxy-dATP analog using NMR. We report on the solution structure of Orf135, which should contribute towards a structural understanding of Orf135 and its interaction with substrates.
authors.affiliation: Graduate School of Science and Engineering, Tokyo Metropolitan University, 1-1 Minamiosawa, Hachioji 192-0397, Japan.
authors.forename: Kumiko
authors.initials: K
authors.lastname: Kawasaki
authors.forename: Teppei
authors.initials: T
authors.lastname: Kanaba
authors.forename: Momoko
authors.initials: M
authors.lastname: Yoneyama
authors.forename: Naoko
authors.initials: N
authors.lastname: Murata-Kamiya
authors.forename: Chojiro
authors.initials: C
authors.lastname: Kojima
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Ito
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kamiya
authors.forename: Masaki
authors.initials: M
authors.lastname: Mishima
mesh_terms: D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000737:chemistry; D020816:Amino Acid Motifs; D001665:Binding Sites; D004926:Escherichia coli / Q000201:enzymology*; D019906:Nuclear Magnetic Resonance, Biomolecular; D011487:Protein Conformation; D011755:Pyrophosphatases / Q000737:chemistry*; D013379:Substrate Specificity; D000096964:Nudix Hydrolases
chemical_list: D000255:Adenosine Triphosphate; D011755:Pyrophosphatases; C522082:2-hydroxyadenosine-5'-triphosphate; C422942:nudix hydrolase Orf135; D000096964:Nudix Hydrolases