title: Early IL-10 production is essential for syngeneic graft acceptance.
abstract: We performed a comparative study and evaluated cellular infiltrates and anti-inflammatory cytokine production at different time-points after syngeneic or allogeneic skin transplantation. We observed an early IL-10 production in syngeneic grafts compared with allografts. This observation prompted us to investigate the role of IL-10 in isograft acceptance. For this, we used IL-10 KO and WT mice to perform syngeneic transplantation, where IL-10 was absent in the graft or in the recipient. The majority of syngeneic grafts derived from IL-10 KO donors did not engraft or was only partially accepted, whereas IL-10 KO mice transplanted with skin from WT donors accepted the graft. We evaluated IL-10 producers in the transplanted skin and observed that epithelial cells were the major source. Taken together, our data show that production of IL-10 by donor cells, but not by the recipient, is determinant for graft acceptance and strongly suggest that production of this cytokine by keratinocytes immediately upon transplantation is necessary for isograft survival.
authors.affiliation: Laboratory of Clinical Immunology, Department of Immunology, Instituto de Ciências Biomédicas, University of São Paulo, São Paulo, Brazil.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Takiishi
authors.forename: Carlos Eduardo
authors.initials: CE
authors.lastname: Tadokoro
authors.forename: Luiz Vicente
authors.initials: LV
authors.lastname: Rizzo
authors.forename: Luciana Vieira
authors.initials: LV
authors.lastname: de Moraes
mesh_terms: D000818:Animals; D006084:Graft Rejection / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D006085:Graft Survival / Q000235:genetics / Q000276:immunology*; D016753:Interleukin-10 / Q000096:biosynthesis* / Q000172:deficiency / Q000235:genetics; D015603:Keratinocytes / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D023421:Models, Animal; D016038:Skin Transplantation / Q000276:immunology*; D014185:Transplantation, Isogeneic / Q000379:methods
chemical_list: C556062:IL10 protein, mouse; D016753:Interleukin-10