title: Monodansylpentane as a blue-fluorescent lipid-droplet marker for multi-color live-cell imaging.
abstract: Lipid droplets (LDs) are dynamic cellular organelles responsible for the storage of neutral lipids, and are associated with a multitude of metabolic syndromes. Here we report monodansylpentane (MDH) as a high contrast blue-fluorescent marker for LDs. The unique spectral properties make MDH easily combinable with other green and red fluorescent reporters for multicolor fluorescence imaging. MDH staining does not apparently affect LD trafficking, and the dye is extraordinarily photo-stable. Taken together MDH represents a reliable tool to use for the investigation of dynamic LD regulation within living cells using fluorescence microscopy.
authors.affiliation: Institute of Biological Chemistry, Academia Sinica, National Taiwan University, Taipei, Taiwan.
authors.forename: Huei-Jiun
authors.initials: HJ
authors.lastname: Yang
authors.forename: Chia-Ling
authors.initials: CL
authors.lastname: Hsu
authors.forename: Jin-Yi
authors.initials: JY
authors.lastname: Yang
authors.forename: Wei Yuan
authors.initials: WY
authors.lastname: Yang
mesh_terms: D041721:3T3-L1 Cells; D000818:Animals; D001692:Biological Transport; D015415:Biomarkers / Q000378:metabolism; D003287:Contrast Media / Q000494:pharmacology; D003619:Dansyl Compounds / Q000494:pharmacology*; D005456:Fluorescent Dyes / Q000494:pharmacology; D005819:Genetic Markers / Q000235:genetics; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D051379:Mice; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D008954:Models, Biological; D008956:Models, Chemical; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D015415:Biomarkers; D003287:Contrast Media; D003619:Dansyl Compounds; D005456:Fluorescent Dyes; D005819:Genetic Markers; D008055:Lipids; C573695:monodansylpentane