title: Anticoccidial effect of halofuginone hydrobromide against Eimeria tenella with associated histology.
abstract: Halofuginone (stenorol) has been used as an effective anticoccidial reagent for decades but very little is known about its mode of action. In this study, chickens were inoculated with Eimeria tenella oocysts on 14-day-old and medicated with halofuginone at days 0, 1, 2, 3, 4, 5 and 6 post inoculations (groups 0, 1, 2, 3, 4, 5 and 6, respectively). Chickens in group 7 were taken as challenge-unmedicated control and in group 8 unchallenged-unmedicated control. The survival rate, body weight gains (BWG), oocysts production, cecal scores, bloody diarrhea and histological examinations were analyzed to evaluate the anticoccidial efficacy of halofuginone and to initially elucidate its mechanisms. Results showed that halofuginone which acted as a coccidiostatic can significantly enhance the BWG, and decrease both the oocyst shedding and cecal destruction caused by E. tenella infection. The histological slide examination noted that halofuginone was effective when provided 0-2 days post inoculation but only partially effective when applied 3-7 days post infection. The second-generation schizonts treated with halofuginone appeared vacuolated and degenerated. It is concluded that halofuginone can inhibit the parasite's invasion of host cecal hypothetical cell at the early stages of life cycle and later disturb the parasite's development by vacuolation of the schizonts. The resulting abnormal schizonts could not divide into schizoites and were eventually eliminated by the host's immune response.
authors.affiliation: Institute of Preventive Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China.
authors.forename: De-Fu
authors.initials: DF
authors.lastname: Zhang
authors.forename: Bing-Bing
authors.initials: BB
authors.lastname: Sun
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Yue
authors.forename: Hai-Jie
authors.initials: HJ
authors.lastname: Yu
authors.forename: Hong-Li
authors.initials: HL
authors.lastname: Zhang
authors.forename: Qian-Jin
authors.initials: QJ
authors.lastname: Zhou
authors.forename: Ai-Fang
authors.initials: AF
authors.lastname: Du
mesh_terms: D000818:Animals; D002645:Chickens; D003048:Coccidiosis / Q000188:drug therapy / Q000469:parasitology / Q000662:veterinary*; D003049:Coccidiostats / Q000627:therapeutic use*; D016786:Eimeria tenella / Q000187:drug effects*; D010880:Piperidines / Q000494:pharmacology*; D011201:Poultry Diseases / Q000188:drug therapy* / Q000469:parasitology; D052999:Quinazolinones / Q000494:pharmacology*
chemical_list: D003049:Coccidiostats; D010880:Piperidines; D052999:Quinazolinones; C010176:halofuginone