title: Antihypertensive effect and antioxidant activity of peptide fractions extracted from Spanish dry-cured ham.
abstract: This study examined the antihypertensive and antioxidant activities of water soluble fractions of a Spanish dry-cured ham extract. Antihypertensive activity of a fractionated peptide extract, by size-exclusion chromatography was determined by measuring changes in systolic blood pressure of spontaneously hypertensive rats after oral administration. Every sample exhibited antihypertensive activity, pooled fractions corresponding to 1700 Da or lower were the most antihypertensive with a decrease of 38.38 mm Hg in systolic blood pressure. In vitro experiments revealed marked in vitro angiotensin I-converting enzyme inhibitory activity in fractions corresponding to these elution volumes. Some of the fractions exhibited great 1,1-diphenyl-2-picrylhydrazyl radical-scavenging activity, ranging from 39% to 92% as well as superoxide ion extinguishing ability with values ranging from 41.67% to 50.27% of the antioxidant activity, suggesting the presence of peptides with antioxidant activity. These findings suggest that Spanish dry-cured ham contains peptides with antioxidant and antihypertensive activities.
authors.affiliation: Instituto de agroquímica y Tecnología de Alimentos (CSIC), Paterna, Valencia, Spain.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Escudero
authors.forename: Maria-Concepcion
authors.initials: MC
authors.lastname: Aristoy
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Nishimura
authors.forename: Keizo
authors.initials: K
authors.lastname: Arihara
authors.forename: Fidel
authors.initials: F
authors.lastname: Toldrá
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D000959:Antihypertensive Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001713:Biphenyl Compounds / Q000378:metabolism; D001794:Blood Pressure / Q000187:drug effects*; D005511:Food Handling; D006973:Hypertension / Q000188:drug therapy* / Q000378:metabolism; D008297:Male; D008461:Meat Products; D010455:Peptides / Q000494:pharmacology / Q000627:therapeutic use*; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism*; D010851:Picrates / Q000378:metabolism; D051381:Rats; D011918:Rats, Inbred SHR; D013030:Spain; D013481:Superoxides / Q000378:metabolism
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000959:Antihypertensive Agents; D001713:Biphenyl Compounds; D010455:Peptides; D010851:Picrates; D013481:Superoxides; C004931:1,1-diphenyl-2-picrylhydrazyl; D007703:Peptidyl-Dipeptidase A