title: Fibre optics sensors in tear electrolyte analysis: towards a novel point of care potassium sensor.
abstract: The diagnosis of ocular disease is increasingly important in optometric practice and there is a need for cost effective point of care assays to assist in that. Although tears are a potentially valuable source of diagnostic information difficulties associated with sample collection and limited sample size together with sample storage and transport have proved major limitations. Progressive developments in electronics and fibre optics together with innovation in sensing technology mean that the construction of inexpensive point of care fibre optic sensing devices is now possible. Tear electrolytes are an obvious family of target analytes, not least to complement the availability of devices that make the routine measurement of tear osmolarity possible in the clinic. In this paper we describe the design, fabrication and calibration of a fibre-optic based electrolyte sensor for the quantification of potassium in tears using the ex vivo contact lens as the sample source. The technology is generic and the same principles can be used in the development of calcium and magnesium sensors. An important objective of this sensor technology development is to provide information at the point of routine optometric examination, which would provide supportive evidence of tear abnormality.
authors.affiliation: Biomaterials Research Unit, School of Engineering and Applied Science, Aston University, UK. biostuff@aston.ac.uk
authors.forename: Daniel
authors.initials: D
authors.lastname: Harvey
authors.forename: Neil W
authors.initials: NW
authors.lastname: Hayes
authors.forename: Brian
authors.initials: B
authors.lastname: Tighe
mesh_terms: D003261:Contact Lenses; D004573:Electrolytes / Q000032:analysis; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005336:Fiber Optic Technology / Q000295:instrumentation*; D006801:Humans; D019095:Point-of-Care Systems; D011188:Potassium / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013057:Spectrum Analysis / Q000295:instrumentation*; D013666:Tears / Q000737:chemistry*; D014159:Transducers
chemical_list: D004573:Electrolytes; D011188:Potassium