title: Prevalence of Ancylostoma braziliense in dogs from Alachua and Marion Counties, Florida, United States.
abstract: A convenience collection of fecal samples from 148 dogs in northern Florida was examined for the presence of Ancylostoma braziliense eggs by using centrifugal sugar flotation and polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP). Of the 148 samples, 64 (43.2%) contained hookworm eggs. DNA from 42 samples was successfully amplified using PCR; using RFLP, 2 samples were identified as containing DNA of A. braziliense (4.8% of the 42 successfully amplified samples).
authors.affiliation: Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853-6401, USA.
authors.forename: Janice L
authors.initials: JL
authors.lastname: Liotta
authors.forename: HeeJeong
authors.initials: H
authors.lastname: Youn
authors.forename: Sarp
authors.initials: S
authors.lastname: Aksel
authors.forename: Stephen E
authors.initials: SE
authors.lastname: Bienhoff
authors.forename: Dwight D
authors.initials: DD
authors.lastname: Bowman
mesh_terms: D000722:Ancylostoma / Q000235:genetics / Q000302:isolation & purification; D000724:Ancylostomiasis / Q000453:epidemiology / Q000662:veterinary*; D000818:Animals; D018509:DNA, Helminth / Q000737:chemistry / Q000302:isolation & purification; D004283:Dog Diseases / Q000453:epidemiology* / Q000469:parasitology; D004285:Dogs; D005243:Feces / Q000469:parasitology; D005431:Florida / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D015995:Prevalence
chemical_list: D018509:DNA, Helminth