title: The toxic effects of Aroclor 1254 exposure on the osteoblastic cell line MC3T3-E1 and its molecular mechanism.
abstract: Polychlorinated biphenyls (PCBs) are still prevalent in the environment despite the fact that they have been banned in many countries for several decades. Recent epidemiologic studies have demonstrated a link between PCBs exposure and pathological alterations of bone tissues. The aim of this study was to investigate the toxic effects of the PCBs mixture Aroclor 1254 on MC3T3-E1 preosteoblasts and explore the underlying molecular mechanism. Different doses of Aroclor 1254 were used to treat MC3T3-E1 and the cell viability, apoptosis, ALP activity, intracellular calcium (Ca2+) level and oxidative stress response were measured. The expression level of related proteins TRPV6, Apaf-1 and Bax was evaluated with Western blot assay. The subcellular distribution of TRPV6 protein was detected with immunofluorescence assay. The results indicated that the higher dose of Aroclor 1254 (>10 mg/L) could inhibit the cell proliferation and induce apoptosis in MC3T3-E1. The ROS level following Aroclor 1254 exposure was elevated with the concentration, while the ALP activity and intracellular calcium (Ca2+) level decreased. After Aroclor 1254 exposure, the expression level of calcium transport related protein TRPV6 was down-regulated, while the expression level of apoptosis related proteins Apaf-1 and Bax up-regulated in a dose dependant manner. The immunofluorescence assay results showed that the expression of TRPV6 in the cytoplasm was greatly suppressed after Aroclor 1254 exposure. In conclusion, Aroclor 1254 exposure could induce toxic effects in MC3T3-E1 as evidenced by inhibition of proliferation, induction of apoptosis and suppression of ALP activity. The ROS production and alteration of intracellular Ca2+ level induced by down-regulation of TRPV6 might involve the toxic effects, and cell apoptosis induced by Aroclor 1254 exposure is associated with the pro-apoptotic Apaf-1 pathway as well as alteration of Bcl-2/Bax ratio.
authors.affiliation: Institute of Environmental Pollution and Health, School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, PR China. peace74839@shu.edu.cn
authors.forename: Jing
authors.initials: J
authors.lastname: An
authors.forename: Wen
authors.initials: W
authors.lastname: Zou
authors.forename: Yufang
authors.initials: Y
authors.lastname: Zhong
authors.forename: Xinyu
authors.initials: X
authors.lastname: Zhang
authors.forename: Minghong
authors.initials: M
authors.lastname: Wu
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Yu
authors.forename: Tianwen
authors.initials: T
authors.lastname: Ye
mesh_terms: D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000032:analysis; D049109:Cell Proliferation / Q000187:drug effects; D020111:Chlorodiphenyl (54% Chlorine) / Q000633:toxicity*; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D017382:Reactive Oxygen Species / Q000378:metabolism; D050916:TRPV Cation Channels / Q000032:analysis
chemical_list: D015220:Calcium Channels; D017382:Reactive Oxygen Species; D050916:TRPV Cation Channels; C446923:Trpv6 protein, mouse; D020111:Chlorodiphenyl (54% Chlorine); D000469:Alkaline Phosphatase; D002118:Calcium