title: Identification of novel and conserved Populus tomentosa microRNA as components of a response to water stress.
abstract: MicroRNAs (miRNAs) are a class of small, non-coding RNAs that play important downregulation roles in plants growth, development, and stress responses. To better identify Populus tomentosa miRNAs and understand the functions of miRNAs in response to water stress (drought and flooding), 152 conserved miRNAs belonging to 36 miRNA families, 8 known but non-conserved miRNAs and 64 candidate novel miRNAs belonging to 54 miRNA families were identified and analyzed from three small RNA (sRNA) libraries (drought treatment, flooding treatment, and control) by high-throughput sequencing combined with qRT-PCR. Significant changes in the expression of 17 conserved miRNA families and nine novel miRNAs were observed in response to drought stress, and in seven conserved miRNA families and five novel miRNAs in response to flooding stress. Both miRNA and miRNA*s were involved in the regulation of plant stress responses. The annotation of the potential targets of miRNAs with differential expression indicate that many types of genes encoding transcription factors, enzymes, and signal transduction components are implicated in the abiotic stress response..
authors.affiliation: National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Biotechnology, Beijing Forestry University, 100083 Beijing, People's Republic of China. renyychina@sina.com
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Ren
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.forename: Yiyun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Kang
authors.forename: Zhiyi
authors.initials: Z
authors.lastname: Zhang
authors.forename: Yanwei
authors.initials: Y
authors.lastname: Wang
mesh_terms: D001483:Base Sequence; D017124:Conserved Sequence; D055864:Droughts; D055868:Floods; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D059014:High-Throughput Nucleotide Sequencing; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D032107:Populus / Q000235:genetics* / Q000502:physiology; D034622:RNA Interference; D018749:RNA, Plant / Q000235:genetics* / Q000378:metabolism; D017423:Sequence Analysis, RNA; D013312:Stress, Physiological; D014867:Water / Q000502:physiology*
chemical_list: D035683:MicroRNAs; D018749:RNA, Plant; D014867:Water