title: In situ electrokinetic enhancement for self-assembled-monolayer-based electrochemical biosensing.
abstract: This study reports a multifunctional electrode approach which directly implements electrokinetic enhancement on a self-assembled-monolayer-based electrochemical sensor for point-of-care diagnostics. Using urinary tract infections as a model system, we demonstrate that electrokinetic enhancement, which involves in situ stirring and heating, can enhance the sensitivity of the strain specific 16S rRNA hybridization assay for 1 order of magnitude and accelerate the time-limiting incubation step with a 6-fold reduction in the incubation time. Since the same electrode platform is used for both electrochemical signal enhancement and electrochemical sensing, the multifunctional electrode approach provides a highly effective strategy toward fully integrated lab-on-a-chip systems for various biomedical applications.
authors.affiliation: Department of Aerospace and Mechanical Engineering, University of Arizona, Tucson, Arizona 85721, United States.
authors.forename: Mandy L Y
authors.initials: ML
authors.lastname: Sin
authors.forename: Tingting
authors.initials: T
authors.lastname: Liu
authors.forename: Jeffrey D
authors.initials: JD
authors.lastname: Pyne
authors.forename: Vincent
authors.initials: V
authors.lastname: Gau
authors.forename: Joseph C
authors.initials: JC
authors.lastname: Liao
authors.forename: Pak Kin
authors.initials: PK
authors.lastname: Wong
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D015374:Biosensing Techniques / Q000295:instrumentation; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D009693:Nucleic Acid Hybridization; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000302:isolation & purification; D012329:RNA, Bacterial / Q000235:genetics / Q000652:urine*; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000652:urine*; D012680:Sensitivity and Specificity; D057790:Staphylococcus saprophyticus / Q000235:genetics / Q000302:isolation & purification; D014552:Urinary Tract Infections / Q000175:diagnosis / Q000652:urine*
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S