title: Tracking protein aggregation and mislocalization in cells with flow cytometry.
abstract: We applied pulse-shape analysis (PulSA) to monitor protein localization changes in mammalian cells by flow cytometry. PulSA enabled high-throughput tracking of protein aggregation, translocation from the cytoplasm to the nucleus and trafficking from the plasma membrane to the Golgi as well as stress-granule formation. Combining PulSA with tetracysteine-based oligomer sensors in a cell model of Huntington's disease enabled further separation of cells enriched with monomers, oligomers and inclusion bodies.
authors.affiliation: Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Melbourne, Victoria, Australia.
authors.forename: Yasmin M
authors.initials: YM
authors.lastname: Ramdzan
authors.forename: Saskia
authors.initials: S
authors.lastname: Polling
authors.forename: Cheryl P Z
authors.initials: CP
authors.lastname: Chia
authors.forename: Ivan H W
authors.initials: IH
authors.lastname: Ng
authors.forename: Angelique R
authors.initials: AR
authors.lastname: Ormsby
authors.forename: Nathan P
authors.initials: NP
authors.lastname: Croft
authors.forename: Anthony W
authors.initials: AW
authors.lastname: Purcell
authors.forename: Marie A
authors.initials: MA
authors.lastname: Bogoyevitch
authors.forename: Dominic C H
authors.initials: DC
authors.lastname: Ng
authors.forename: Paul A
authors.initials: PA
authors.lastname: Gleeson
authors.forename: Danny M
authors.initials: DM
authors.lastname: Hatters
mesh_terms: D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D005434:Flow Cytometry / Q000379:methods*; D006056:Golgi Apparatus / Q000378:metabolism; D006801:Humans; D000071058:Huntingtin Protein; D006816:Huntington Disease / Q000378:metabolism*; D002479:Inclusion Bodies / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D021381:Protein Transport
chemical_list: C086055:HTT protein, human; D000071058:Huntingtin Protein; D009419:Nerve Tissue Proteins