title: Sequencing-based molecular typing of treponema pallidum strains in the Czech Republic: all identified genotypes are related to the sequence of the SS14 strain.
abstract: A set of 415 clinical samples isolated from 294 patients suspected of having syphilis collected in the Czech Republic between 2004 and 2010 was tested for the presence of treponemal DNA. Standard serological tests showed that 197 patients were syphilis-seropositive and 97 patients were syphilis-seronegative. In each sample, PCR tests for polA (TP0105), tmpC (TP0319), TP0136, TP0548 and 23S rRNA genes were performed. Samples taken from 91 patients were PCR-positive. Molecular typing of treponemal DNA was based on the sequencing of TP0136, TP0548 and 23S rRNA genes. Treponemal DNA was typeable in samples taken from 64 PCR-positive patients and 9 different genotypes were found. The proportion of treponemal strains resistant to macrolide antibiotics was 37.3%. In the DNA samples taken from 39 patients, a parallel treponemal typing approved by Centers for Disease Control and Prevention was performed. The variants of arp and tpr genes appear to combine independently with sequence variants of TP0136, TP0548 and 23S rRNA genes.
authors.affiliation: Department of Biology, Faculty of Medicine, Masaryk University, Kamenice 5, Building A6, 625 00 Brno, Czech Republic.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Flasarová
authors.forename: Petra
authors.initials: P
authors.lastname: Pospíšilová
authors.forename: Lenka
authors.initials: L
authors.lastname: Mikalová
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Vališová
authors.forename: Eliška
authors.initials: E
authors.lastname: Dastychová
authors.forename: Radim
authors.initials: R
authors.lastname: Strnadel
authors.forename: Ivana
authors.initials: I
authors.lastname: Kuklová
authors.forename: Vladana
authors.initials: V
authors.lastname: Woznicová
authors.forename: Hana
authors.initials: H
authors.lastname: Zákoucká
authors.forename: David
authors.initials: D
authors.lastname: Šmajs
mesh_terms: D000328:Adult; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D001483:Base Sequence; D018153:Czech Republic / Q000453:epidemiology; D004269:DNA, Bacterial / Q000032:analysis*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012338:RNA, Ribosomal, 23S / Q000235:genetics*; D021521:Ribotyping / Q000379:methods; D017422:Sequence Analysis, DNA; D013587:Syphilis / Q000175:diagnosis / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology*; D014210:Treponema pallidum / Q000187:drug effects / Q000235:genetics* / Q000276:immunology
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D012338:RNA, Ribosomal, 23S