title: Antisense-mediated exon-skipping to induce gene knockdown.
abstract: Exon-skipping antisense oligonucleotides (ASOs) can be used to knockdown the expression of an undesired gene or specific gene isoform. This chapter discusses the potential therapeutic applications of the technique and provides a sample protocol for inducing exon-skipping in Apolipoprotein B in vitro, as well as a protocol for quantifying exon-skipping using real-time PCR.
authors.affiliation: Department of Endocrinology, UCL Medical School, London, UK.
authors.forename: Petra
authors.initials: P
authors.lastname: Disterer
authors.forename: Bernard
authors.initials: B
authors.lastname: Khoo
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D001055:Apolipoproteins B / Q000235:genetics*; D002460:Cell Line; D002784:Cholesterol / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000235:genetics; D005091:Exons; D055785:Gene Knockdown Techniques; D006801:Humans; D016376:Oligonucleotides, Antisense / Q000235:genetics* / Q000627:therapeutic use; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics; D014162:Transfection
chemical_list: D001055:Apolipoproteins B; D002784:Cholesterol; D051546:Cyclooxygenase 2; D016376:Oligonucleotides, Antisense; D012313:RNA; D018719:Erb-b2 Receptor Tyrosine Kinases