title: Study on the DNA repair gene XRCC1 and XRCC3 polymorphism in prediction and prognosis of hepatocellular carcinoma risk.
abstract: OBJECTIVE
  We conducted a case-control study in China to clarify the association between XRCC1-Arg-399Gin polymorphism and HCC risk.
  
  
  METHODS
  A total of 150 cases and 158 controls were selected from May 2008 to May 2010. XRCC1-Arg399Gin and XRCC3-Thr241Met polymorphisms were based upon duplex polymerase-chain-reaction with the confronting-two-pair primer (PCR-CTPP) method. All analysis was performed by using the STATA statistical package.
  
  
  RESULTS
  A significant increased risk of HCC was associated with XRCC1 399Arg/Gin, and a heavy risk of HCC was also found in individuals with XRCC3 241Met/Met genotypes. A significant association was found between positive HBsAg and Arg/Gin. XRCC3 Thr/Met genotypes had a significant positive association with HBsAg (+) and a heavy risk of HCC was found in HBsAg (+) individuals with XRCC3 Met/Met genotype. Individuals carrying XRCC1 Gin/Gin genotypes showed significantly lower median survival than XRCC1 Arg/ Arg genotypes and significant hazard ratio (HR=l.38, 95% CI=l.04-1.84) was found. Meanwhile, we found a moderate HR for XRCC3 Thr/Met (HR=l.96, 95% CI=l.23-3.15) and a heavy HR for XRCC3 Met/Met (HR=2.98, 95% CI=1.77-7.54).
  
  
  CONCLUSIONS
  In conclusion, we observed that XRCC1-Arg399Gln and XRCC3-Thr241Met polymorphism is associated with susceptibility to HCC and XRCC1 Gin allele and XRCC3 Met allele genotype showed significant poor prognosis of HCC.
authors.affiliation: Department of Anesthesiology, The First Affiliated Hospital of Henan Science and Technology University, Luoyang, China.
authors.forename: Xuechang
authors.initials: X
authors.lastname: Han
authors.forename: Qunzhi
authors.initials: Q
authors.lastname: Xing
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
authors.forename: Junjun
authors.initials: J
authors.lastname: Sun
authors.forename: Huihui
authors.initials: H
authors.lastname: Ji
authors.forename: Pan
authors.initials: P
authors.lastname: Huazheng
authors.forename: Liang
authors.initials: L
authors.lastname: Jun
mesh_terms: D000328:Adult; D000428:Alcohol Drinking / Q000009:adverse effects; D015415:Biomarkers / Q000097:blood; D006528:Carcinoma, Hepatocellular / Q000209:etiology / Q000235:genetics* / Q000401:mortality / Q000473:pathology / Q000628:therapy; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D002681:China; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006509:Hepatitis B / Q000150:complications / Q000175:diagnosis; D006514:Hepatitis B Surface Antigens / Q000097:blood; D006801:Humans; D053208:Kaplan-Meier Estimate; D008113:Liver Neoplasms / Q000209:etiology / Q000235:genetics* / Q000401:mortality / Q000473:pathology / Q000628:therapy; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D011379:Prognosis; D016016:Proportional Hazards Models; D012307:Risk Factors; D000076105:X-ray Repair Cross Complementing Protein 1
chemical_list: D015415:Biomarkers; D004268:DNA-Binding Proteins; D006514:Hepatitis B Surface Antigens; D000076105:X-ray Repair Cross Complementing Protein 1; C110901:X-ray repair cross complementing protein 3; C000619371:XRCC1 protein, human