title: Modulation of the human preadipocyte mitochondrial activity by beta-carotene.
abstract: Increased ROS generation by the overload by metabolic substrates mitochondria paralleled by decrease of antioxidant activity are typical events found in metabolic syndrome and diabetes type 2. Metabolites of beta-carotene (BC) such as retinoic acid (RA), as well as low concentration of reactive oxygen species (ROS) modify the mitochondrial bioenergetic function. The aim of the study was to investigate the effect of beta-carotene on mitochondrial activity in human preadipocytes. BC used in concentrations, 10 or 30 µM, decreased mitochondrial membrane potential, inhibited mitochondrial respiration and decreased cellular ATP content. We conclude, that BC, the known antioxidant may decrease oxidative phosphorylation capacity of mitochondria.
authors.affiliation: Department of Clinical Biochemistry, Jagiellonian University Medical College, Kraków, Poland. agasliw@interia.pl
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Sliwa
authors.forename: Joanna
authors.initials: J
authors.lastname: Góralska
authors.forename: Urszula
authors.initials: U
authors.lastname: Czech
authors.forename: Anna
authors.initials: A
authors.lastname: Gruca
authors.forename: Anna
authors.initials: A
authors.lastname: Polus
authors.forename: Barbara
authors.initials: B
authors.lastname: Zapała
authors.forename: Aldona
authors.initials: A
authors.lastname: Dembińska-Kieć
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism*; D002460:Cell Line; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism*; D010085:Oxidative Phosphorylation / Q000187:drug effects; D019207:beta Carotene / Q000494:pharmacology*
chemical_list: D019207:beta Carotene; D000255:Adenosine Triphosphate