title: The differential proliferative ability of satellite cells in Lantang and Landrace pigs.
abstract: Here, for the first time, we evaluate the hypothesis that the proliferative abilities of satellite cells (SCs) isolated from Lantang (indigenous Chinese pigs) and Landrace pigs, which differ in muscle characteristics, are different. SCs were isolated from the longissimus dorsi muscle of neonatal Lantang and Landrace pigs. Proliferative ability was estimated by the count and proliferative activity of viable cells using a hemocytometer and MTT assay at different time points after seeding, respectively. Cell cycle information was detected by flow cytometry. Results showed that there was a greater (P<0.05) number of SCs in Lantang pigs compared with Landrace pigs after 72 h of culture. The percentage of cell population in S phase and G(2)/M phases in Lantang pigs were higher (P<0.05), while in G(0)/G(1) phase was lower (P<0.05) in comparison with the Landrace pigs. The mRNA abundances of MyoD, Myf5, myogenin and Pax7 in SCs from Lantang pigs were higher (P<0.05), while those of myostatin, Smad3 and genes in the mammalian target of rapamycin (mTOR) pathway (with the exception of 4EBP1) were lower (P<0.05) than the Landrace pigs. Protein levels of MyoD, myogenin, myostatin, S6K, phosphorylated mTOR and phosphorylated eIF4E were consistent with the corresponding mRNA abundance. Collectively, these findings suggested that SCs in the two breeds present different proliferative abilities, and the proliferative potential of SCs in Lantang pigs is higher than in Landrace pigs.
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou, Guangdong Province, China. xqwang@scau.edu.cn
authors.forename: Xiu-qi
authors.initials: XQ
authors.lastname: Wang
authors.forename: Wei-jun
authors.initials: WJ
authors.lastname: Yang
authors.forename: Zhou
authors.initials: Z
authors.lastname: Yang
authors.forename: Gang
authors.initials: G
authors.lastname: Shu
authors.forename: Song-bo
authors.initials: SB
authors.lastname: Wang
authors.forename: Qing-yan
authors.initials: QY
authors.lastname: Jiang
authors.forename: Li
authors.initials: L
authors.lastname: Yuan
authors.forename: Tong-shan
authors.initials: TS
authors.lastname: Wu
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002453:Cell Cycle / Q000502:physiology; D049109:Cell Proliferation; D005434:Flow Cytometry; D018482:Muscle, Skeletal / Q000166:cytology*; D017570:MyoD Protein / Q000378:metabolism; D051787:Myogenic Regulatory Factor 5 / Q000378:metabolism; D018008:Myogenin / Q000378:metabolism; D055435:Myostatin / Q000378:metabolism; D051763:PAX7 Transcription Factor / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D032496:Satellite Cells, Skeletal Muscle / Q000502:physiology*; D051900:Smad3 Protein / Q000378:metabolism; D013045:Species Specificity; D034421:Sus scrofa / Q000502:physiology*; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D017570:MyoD Protein; D051787:Myogenic Regulatory Factor 5; D018008:Myogenin; D055435:Myostatin; D051763:PAX7 Transcription Factor; D051900:Smad3 Protein; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue