title: Identification of marker genes for lipid-lowering effect of a short-chain fructooligosaccharide by DNA microarray analysis.
abstract: Administration of short-chain fructooligosaccharide (scFOS) is known to lower serum triglyceride levels in rats fed a high-fat diet, but the molecular mechanisms remain unclear. This study aimed to identify marker genes for lipid-lowering effect of scFOS administration. The changes in hepatic gene expressions in rats fed scFOS were investigated using DNA microarray and quantitative RT-PCR analysis. The DNA microarray showed that phytanoyl-CoA 2-hydroxylase 2 (Phyh2), lipoprotein lipase (Lpl) and tyrosine aminotransferase (Tat) were significantly affected by scFOS administration (p < .05). Since Lpl is involved in lipid metabolism, the up-regulation of Lpl in the liver can be a potential marker of the lipid-lowering effect of scFOS.
authors.affiliation: Food and Health R&D Laboratories, Meiji Seika Kaisha, Ltd., Chiyoda, Sakado-shi, Saitama, Japan. tomoyuki_fukasawa@meiji.co.jp
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Fukasawa
authors.forename: Koichiro
authors.initials: K
authors.lastname: Murashima
authors.forename: Tomoko
authors.initials: T
authors.lastname: Nemoto
authors.forename: Ichiro
authors.initials: I
authors.lastname: Matsumoto
authors.forename: Jinichiro
authors.initials: J
authors.lastname: Koga
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Kubota
authors.forename: Minoru
authors.initials: M
authors.lastname: Kanegae
mesh_terms: D000818:Animals; D003065:Coenzyme A / Q000378:metabolism; D004247:DNA / Q000032:analysis; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D004798:Enzymes / Q000235:genetics* / Q000378:metabolism; D005632:Fructose / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D005819:Genetic Markers; D000960:Hypolipidemic Agents / Q000494:pharmacology*; D050356:Lipid Metabolism / Q000187:drug effects; D008071:Lipoprotein Lipase / Q000235:genetics / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D046228:Microarray Analysis; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000378:metabolism; D009844:Oligosaccharides / Q000494:pharmacology*; D010831:Phytanic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014280:Triglycerides / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D014444:Tyrosine Transaminase / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D004791:Enzyme Inhibitors; D004798:Enzymes; D005819:Genetic Markers; D000960:Hypolipidemic Agents; D009844:Oligosaccharides; D014280:Triglycerides; C089917:phytanoyl-coenzyme A; D010831:Phytanic Acid; D005632:Fructose; D004247:DNA; D006899:Mixed Function Oxygenases; C491171:Phyh protein, rat; D014444:Tyrosine Transaminase; D008071:Lipoprotein Lipase; D003065:Coenzyme A