title: A comparison of HLA genotype with inflammation in uveal melanoma.
abstract: OBJECTIVE
  Human leukocyte antigen (HLA) polymorphisms have been associated with the development of autoimmune diseases. In uveal melanoma, a high expression of HLA classes I and II, and infiltration with lymphocytes and macrophages are associated with a bad prognosis. Inflammation has an important role in this malignancy. The goal of our study was to determine whether specific HLA alleles are associated with increased inflammation.
  
  
  METHODS
  Records were analyzed of 45 patients who underwent enucleation for uveal melanoma. HLA typing, tumor HLA expression and tumor macrophage infiltration were determined in each case.
  
  
  RESULTS
  Before correction for multiple testing, macrophage infiltration was less in HLA-A2 positive patients. Patients with HLA-DR6 had a higher tumor cell expression of HLA-DR. After correction for the number of analyses, no associations remained statistically significant.
  
  
  CONCLUSIONS
  The results before correction suggest that the HLA genotype may influence inflammation as indicated by HLA expression and macrophage infiltration in uveal melanoma. However, after correction this association did not prove significant.
authors.affiliation: Department of Ophthalmology, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: T Huibertus
authors.initials: TH
authors.lastname: van Essen
authors.forename: Inge H G
authors.initials: IH
authors.lastname: Bronkhorst
authors.forename: Willem
authors.initials: W
authors.lastname: Maat
authors.forename: Willem
authors.initials: W
authors.lastname: Verduyn
authors.forename: Dave L
authors.initials: DL
authors.lastname: Roelen
authors.forename: Gregorius P M
authors.initials: GP
authors.lastname: Luyten
authors.forename: Martine J
authors.initials: MJ
authors.lastname: Jager
mesh_terms: D000483:Alleles; D002893:Chromosomes, Human, Pair 3 / Q000378:metabolism; D015353:Eye Enucleation; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D005838:Genotype; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D006650:Histocompatibility Testing; D006801:Humans; D007249:Inflammation / Q000276:immunology*; D008264:Macrophages / Q000502:physiology*; D008297:Male; D008545:Melanoma / Q000235:genetics* / Q000276:immunology / Q000601:surgery; D008875:Middle Aged; D014604:Uveal Neoplasms / Q000235:genetics* / Q000276:immunology / Q000601:surgery
chemical_list: D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II