title: Low molecular weight apple polysaccharides induced cell cycle arrest in colorectal tumor.
abstract: Dietary components play an important role in cancer prevention. Many ingredients from apples have been proven to have antitumor potency. We thus made low molecular weight apple polysaccharides (LMWAP) and evaluated the effects of it on colorectal cancer (CRC). The effects of LMWAP on human colon carcinoma cells (HT-29) were evaluated using a microarray. Then, cell-cycle distribution was measured by flow cytometric analysis. A colitis-associated colorectal cancer mouse model was used to assess the effect of LMWAP on in vivo CRC prevention. Treatment of HT-29 cells with LMWAP resulted in 333 genes expression over cutoff values (≥2-fold). Further analysis demonstrated that pathways of cell cycle were mainly influenced. At the concentrations from 0.001 to 0.1 mg/mL, LMWAP induced a G(0)/G(1) phase block in HT-29 cells in a dose-dependent way. In vivo studies revealed that administration of LMWAP could protect ICR mice against CRC effectively. The results of Western blot suggested LMWAP induced cell-cycle arrest in a p53 independent manner. These data indicate that LMWAP could inhibit the development of CRC through affecting cell cycle, and it has potential for clinical prevention for colon cancer.
authors.affiliation: Department of Pharmacology, School of Pharmacy, the Fourth Military Medical University, Key Laboratory of Gastrointestinal Pharmacology of Chinese Materia Medica of the State Administration of Traditional Chinese Medicine, Shaanxi, P.R. China.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Li
authors.forename: Lin
authors.initials: L
authors.lastname: Mei
authors.forename: Yinbo
authors.initials: Y
authors.lastname: Niu
authors.forename: Yang
authors.initials: Y
authors.lastname: Sun
authors.forename: Haitao
authors.initials: H
authors.lastname: Huang
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.forename: Xianghe
authors.initials: X
authors.lastname: Kong
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.forename: Zhiquan
authors.initials: Z
authors.lastname: Li
authors.forename: Qibing
authors.initials: Q
authors.lastname: Mei
mesh_terms: D000818:Animals; D059447:Cell Cycle Checkpoints / Q000187:drug effects*; D015179:Colorectal Neoplasms / Q000473:pathology*; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D019073:HT29 Cells / Q000187:drug effects; D006801:Humans; D008297:Male; D027845:Malus / Q000737:chemistry*; D051379:Mice; D008813:Mice, Inbred ICR; D008970:Molecular Weight; D020411:Oligonucleotide Array Sequence Analysis; D010936:Plant Extracts / Q000494:pharmacology; D011134:Polysaccharides / Q000032:analysis* / Q000494:pharmacology*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D010936:Plant Extracts; D011134:Polysaccharides; D016159:Tumor Suppressor Protein p53