title: The movement protein of barley yellow dwarf virus-GAV self-interacts and forms homodimers in vitro and in vivo.
abstract: The 17-kDa movement protein (MP) of the GAV strain of barley yellow dwarf virus (BYDV-GAV) can bind the viral RNA and target to the nucleus. However, much less is known about the active form of the MP in planta. In this study, the ability of the MP to self-interact was analyzed by yeast two-hybrid assay and bimolecular fluorescence complementation. The BYDV-GAV MP has a strong potential to self-interact in vitro and in vivo, and self-interaction was mediated by the N-terminal domain spanning the second α-helix (residues 17-39). Chemical cross-linking and heterologous MP expression from a pea early browning virus (PEBV) vector further showed that MP self-interacts to form homodimers in vitro and in planta. Interestingly, the N-terminal domain necessary for MP self-interaction has previously been identified as important for nuclear targeting. Based on these findings, a functional link between MP self-interaction and nuclear targeting is discussed.
authors.affiliation: Key Laboratory of Physiology, Ecology and Genetic Improvement of Food Crops in Henan Province, College of Life Science, Henan Agricultural University, Zhengzhou 450002, People's Republic of China. xiazl08@163.com
authors.forename: Zongliang
authors.initials: Z
authors.lastname: Xia
authors.forename: Rufei
authors.initials: R
authors.lastname: Cao
authors.forename: Kaile
authors.initials: K
authors.lastname: Sun
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
mesh_terms: D015967:Gene Expression Regulation, Viral / Q000502:physiology*; D005816:Genetic Complementation Test / Q000379:methods; D017793:Luteovirus / Q000235:genetics / Q000378:metabolism*; D010935:Plant Diseases / Q000821:virology*; D053830:Plant Viral Movement Proteins / Q000235:genetics / Q000378:metabolism*; D055503:Protein Multimerization; D012441:Saccharomyces cerevisiae; D014026:Nicotiana / Q000821:virology*; D020798:Two-Hybrid System Techniques
chemical_list: D053830:Plant Viral Movement Proteins