title: Induction of humoral responses to BHV-1 glycoprotein D expressed by HSV-1 amplicon vectors.
abstract: Herpes simplex virus type-1 (HSV-1) amplicon vectors are versatile and useful tools for transferring genes into cells that are capable of stimulating a specific immune response to their expressed antigens. In this work, two HSV-1-derived amplicon vectors were generated. One of these expressed the full-length glycoprotein D (gD) of bovine herpesvirus 1 while the second expressed the truncated form of gD (gDtr) which lacked the trans-membrane region. After evaluating gD expression in the infected cells, the ability of both vectors to induce a specific gD immune response was tested in BALB/c mice that were intramuscularly immunized. Specific serum antibody responses were detected in mice inoculated with both vectors, and the response against truncated gD was higher than the response against full-length gD. These results reinforce previous findings that HSV-1 amplicon vectors can potentially deliver antigens to animals and highlight the prospective use of these vectors for treating infectious bovine rhinotracheitis disease.
authors.affiliation: Sección Virología, Facultad de Ciencias, Universidad de la República, Montevideo 11600, Uruguay.
authors.forename: Andrea Maria
authors.initials: AM
authors.lastname: Blanc
authors.forename: Mabel Beatriz
authors.initials: MB
authors.lastname: Berois
authors.forename: Lorena Magalí
authors.initials: LM
authors.lastname: Tomé
authors.forename: Alberto L
authors.initials: AL
authors.lastname: Epstein
authors.forename: Juan Ramón
authors.initials: JR
authors.lastname: Arbiza
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D015153:Blotting, Western / Q000662:veterinary; D002417:Cattle; D005260:Female; D005822:Genetic Vectors / Q000276:immunology*; D007242:Herpesvirus 1, Bovine / Q000235:genetics / Q000276:immunology*; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000276:immunology*; D056724:Immunity, Humoral / Q000276:immunology; D007114:Immunization / Q000379:methods / Q000662:veterinary; D007241:Infectious Bovine Rhinotracheitis / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests / Q000662:veterinary; D013047:Specific Pathogen-Free Organisms; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D014764:Viral Proteins; D014765:Viral Vaccines; C046998:bovine herpesvirus type-1 glycoproteins