title: The Ala-9Val (Mn-SOD) and Arg213Gly (EC-SOD) polymorphisms in the pathogenesis of preeclampsia in Romanian women: association with the severity and outcome of preeclampsia.
abstract: OBJECTIVE
  To check whether individual or combined mutated genotypes for Ala-9Val (Mn-SOD) and Arg213Gly (EC-SOD) are associated with preeclampsia; to check the influence of the mutated genotypes on the degree of severity and perinatal outcome of preeclampsia.
  
  
  METHODS
  We genotyped 97 pregnant women (47 with preeclampsia and 50 normal pregnant women) using PCR-RFLP analysis.
  
  
  RESULTS
  The Val/Val (Mn-SOD) genotype (OR 5.99, p = 0.004) but not the Gly/Gly (EC-SOD) genotype (OR 4.23, p = 0.027) was significantly associated with preeclampsia. Higher frequency of both polymorphisms in women with preeclampsia (42.55%) compared to normal pregnant women (8%). Higher frequency of women diagnosed with PIH (27.27%, OR 4.31), mild (50%, OR 11.5) and severe preeclampsia (37.5%, OR 6.9) positive for both polymorphism compared to control women (8%). There was a statistically significant difference in gestational age at delivery according to Mn-SOD (Ala/Ala vs. Val/Val, 39 ± 1.41 weeks vs. 32.77 ± 3.7 weeks) and EC-SOD genotypes (Arg/Arg vs. Gly/Gly, 37.05 ± 3.18 weeks vs. 31.5 ± 3.84 weeks). There also was a statistically significant difference in birth weight according to Mn-SOD (grams, Ala/Ala vs. Val/Val, 3080 ± 481.66 vs. 2376.92 ± 916.88) and EC-SOD genotypes (grams, Arg/Arg vs. Gly/Gly, 2934.09 ± 662.14 vs. 2080 ± 721.19).
  
  
  CONCLUSIONS
  Our study demonstrates a relationship between these two mutated genes, the clinical severity and the perinatal outcome of preeclampsia.
authors.affiliation: Department of Medical Biochemistry, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania. luciamariaprocopciuc@yahoo.com
authors.forename: Lucia Maria
authors.initials: LM
authors.lastname: Procopciuc
authors.forename: Gabriela
authors.initials: G
authors.lastname: Caracostea
authors.forename: Georgiana
authors.initials: G
authors.lastname: Nemeti
authors.forename: Cristina
authors.initials: C
authors.lastname: Drugan
authors.forename: Ileana
authors.initials: I
authors.lastname: Olteanu
authors.forename: Florin
authors.initials: F
authors.lastname: Stamatian
mesh_terms: D000328:Adult; D019943:Amino Acid Substitution; D016022:Case-Control Studies; D005260:Female; D005838:Genotype; D006801:Humans; D009154:Mutation; D011110:Polymorphism, Genetic; D011225:Pre-Eclampsia / Q000235:genetics*; D011247:Pregnancy; D011256:Pregnancy Outcome; D012383:Romania; D013482:Superoxide Dismutase / Q000235:genetics*; D055815:Young Adult
chemical_list: D013482:Superoxide Dismutase