title: [Application of genome-wide microarray for screening genes related to peritoneal metastasis of colorectal cancer].
abstract: OBJECTIVE
  To screen genes related to peritoneal metastasis of colorectal cancer.
  
  
  METHODS
  Specimens of primary cancer and normal mucosa tissues were collected from 3 patients with peritoneal metastasis of colorectal cancer. The total RNA were extracted and inversely transcribed into cDNA to synthesize aRNA using in vitro RNA synthesis. The synthesized aRNA, after labeling with Cy3, were hybridized with the whole human genome oligo microarray. The Empirical Bayes method was used to screen the differentially expressed genes, followed by confirmation of the selected genes by semi-quantitative RT-PCR.
  
  
  RESULTS
  With a threshold of P≤0.05, a total of 105 differentially expressed genes were identified in primary cancer lesions, including 42 up-regulated and 63 down-regulated genes. Three of the up-regulated genes (S100P, PRDX1 and SLPI) were selected and confirmed by RT-PCR, which yielded results consistent with those from gene microarray.
  
  
  CONCLUSIONS
  Gene microarray technique can provide valuable clues for locating the tumor markers of peritoneal metastasis in colorectal cancer patients.
authors.affiliation: Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. liufeng19761002@163.com
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.forename: Jiu-Bing
authors.initials: JB
authors.lastname: Guo
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Shen
authors.forename: Ting-Yu
authors.initials: TY
authors.lastname: Mu
authors.forename: Peng-Ke
authors.initials: PK
authors.lastname: Zhi
authors.forename: Guo-Xin
authors.initials: GX
authors.lastname: Li
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000556:secondary*; D002288:Adenocarcinoma, Mucinous / Q000235:genetics / Q000556:secondary; D000328:Adult; D000368:Aged; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling; D055106:Genome-Wide Association Study; D006801:Humans; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010534:Peritoneal Neoplasms / Q000235:genetics* / Q000556:secondary*; D054464:Peroxiredoxins / Q000235:genetics / Q000378:metabolism; D053481:Secretory Leukocyte Peptidase Inhibitor / Q000235:genetics / Q000378:metabolism
chemical_list: D002135:Calcium-Binding Proteins; D009363:Neoplasm Proteins; C103003:S100P protein, human; C506814:SLPI protein, human; D053481:Secretory Leukocyte Peptidase Inhibitor; C515940:PRDX1 protein, human; D054464:Peroxiredoxins