title: Label-free fluorescent assays based on aptamer-target recognition.
abstract: Label-free fluorescent assays were developed based on the competition of intramolecular DNA hybridization and aptamer-target binding. Using small molecule adenosine triphosphate (ATP) and biomacro-molecule thrombin as model targets, our design was proved to be a general method with good sensitivity and high selectivity.
authors.affiliation: Guangdong Provincial Key Laboratory of Chemical Biology, Graduate School at Shenzhen, Tsinghua University, Shenzhen, 518055, P. R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Tan
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Xie
authors.forename: Rui
authors.initials: R
authors.lastname: Zhao
authors.forename: Chunyan
authors.initials: C
authors.lastname: Tan
authors.forename: Yuyang
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D052160:Benzothiazoles; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D003959:Diamines; D005456:Fluorescent Dyes / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D009930:Organic Chemicals / Q000737:chemistry; D011804:Quinolines; D013050:Spectrometry, Fluorescence; D013917:Thrombin / Q000032:analysis
chemical_list: D052157:Aptamers, Nucleotide; D052160:Benzothiazoles; D003959:Diamines; D005456:Fluorescent Dyes; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I; D000255:Adenosine Triphosphate; D004247:DNA; D013917:Thrombin