title: Effect of puerarin on the release of interleukin-8 in co-culture of human bronchial epithelial cells and neutrophils.
abstract: OBJECTIVE
  To investigate the effect of puerarin on interleukin (IL)-8 mRNA expression and the protein release in the co-culture of human bronchial epithelial (BEAS-2B) cells and human neutrophils.
  
  
  METHODS
  BEAS-2B cells and neutrophills were cultured separately and co-cultured with puerarin (50, 100, and 200 μg/mL) for a predetermined time. Cytokines in culture supernatant were evaluated by protein array and IL-8 quantified by enzyme-linked immunosorbent assay (ELISA). IL-8 mRNA expression was evaluated by real-time quantitative polymerase chain reaction (real-time qPCR).
  
  
  RESULTS
  The co-culture of BEAS-2B cells and neutrophils exhibited synergistic effects on IL-8 mRNA expression in BEAS-2B cells, but not in neutrophils after 12 h incubation (P<0.01), as compared with that in BEAS-2B cells or neutrophils alone. IL-8 protein release in the culture supernatant was obviously elevated when BEAS-2B cells were co-cultured with human neutrophils as compared with that in the supernatant of BEAS-2B cells or neutrophils alone after incubated for 2, 6, 12, and 18 h (P<0.01). Treatment with puerarin could significantly down-regulate the expression of IL-8 mRNA in BEAS-2B cells and IL-8 release in the supernatant of the co-culture of BEAS-2B cells and neutrophils (P<0.01).
  
  
  CONCLUSIONS
  Puerarin could exhibit anti-inflammatory activity by suppressing IL-8 production from the co-culture of human bronchial epithelial cells and neutrophils.
authors.affiliation: Department of Clinical Laboratory Medicine, Chinese People's Liberation Army General Hospital and Postgraduate Medical School, Beijing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Pang
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Lan
authors.forename: Cheng-Bin
authors.initials: CB
authors.lastname: Wang
mesh_terms: D000328:Adult; D001980:Bronchi / Q000166:cytology*; D002450:Cell Communication / Q000187:drug effects; D002460:Cell Line; D018920:Coculture Techniques; D004847:Epithelial Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D005453:Fluorescence; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism*; D007529:Isoflavones / Q000494:pharmacology*; D009504:Neutrophils / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D016209:Interleukin-8; D007529:Isoflavones; D012333:RNA, Messenger; C033607:puerarin