title: Gene expression profiles in genetically different mice infected with Toxoplasma gondii: ALDH1A2, BEX2, EGR2, CCL3 and PLAU.
abstract: Toxoplasma gondii can modulate host cell gene expression; however, determining gene expression levels in intermediate hosts after T. gondii infection is not known much. We selected 5 genes (ALDH1A2, BEX2, CCL3, EGR2 and PLAU) and compared the mRNA expression levels in the spleen, liver, lung and small intestine of genetically different mice infected with T. gondii. ALDH1A2 mRNA expressions of both mouse strains were markedly increased at day 1-4 postinfection (PI) and then decreased, and its expressions in the spleen and lung were significantly higher in C57BL/6 mice than those of BALB/c mice. BEX2 and CCR3 mRNA expressions of both mouse strains were significantly increased from day 7 PI and peaked at day 15-30 PI (P<0.05), especially high in the spleen liver or small intestine of C57BL/6 mice. EGR2 and PLAU mRNA expressions of both mouse strains were significantly increased after infection, especially high in the spleen and liver. However, their expression patterns were varied depending on the tissue and mouse strain. Taken together, T. gondii-susceptible C57BL/6 mice expressed higher levels of these 5 genes than did T. gondii-resistant BALB/c mice, particularly in the spleen and liver. And ALDH1A2 and PLAU expressions were increased acutely, whereas BEX2, CCL3 and EGR2 expressions were increased lately. Thus, these demonstrate that host genetic factors exert a strong impact on the expression of these 5 genes and their expression patterns were varied depending on the gene or tissue.
authors.affiliation: Department of Infection Biology, Research Institute for Medical Science, Chungnam National University School of Medicine, Daejeon 301-131, Korea.
authors.forename: Hassan Ahmed Hassan Ahmed
authors.initials: HA
authors.lastname: Ismail
authors.forename: Juan-Hua
authors.initials: JH
authors.lastname: Quan
authors.forename: Zhou
authors.initials: Z
authors.lastname: Wei
authors.forename: In-Wook
authors.initials: IW
authors.lastname: Choi
authors.forename: Guang-Ho
authors.initials: GH
authors.lastname: Cha
authors.forename: Dae-Whan
authors.initials: DW
authors.lastname: Shin
authors.forename: Young-Ha
authors.initials: YH
authors.lastname: Lee
authors.forename: Chang-June
authors.initials: CJ
authors.lastname: Song
mesh_terms: D000444:Aldehyde Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D000080924:Aldehyde Dehydrogenase 1 Family; D000818:Animals; D001921:Brain / Q000378:metabolism / Q000469:parasitology; D054405:Chemokine CCL3 / Q000235:genetics* / Q000378:metabolism; D051767:Early Growth Response Protein 2 / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D006801:Humans; D008168:Lung / Q000378:metabolism / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D009928:Organ Specificity; D050697:Retinal Dehydrogenase; D013154:Spleen / Q000378:metabolism / Q000821:virology; D014122:Toxoplasma / Q000502:physiology*; D014123:Toxoplasmosis / Q000235:genetics* / Q000378:metabolism / Q000469:parasitology; D014568:Urokinase-Type Plasminogen Activator / Q000235:genetics* / Q000378:metabolism
chemical_list: C502261:Bex2 protein, mouse; C496534:Ccl3 protein, mouse; D054405:Chemokine CCL3; D051767:Early Growth Response Protein 2; C497327:Egr2 protein, mouse; D009419:Nerve Tissue Proteins; D000080924:Aldehyde Dehydrogenase 1 Family; D000444:Aldehyde Dehydrogenase; C512722:Aldh1a2 protein, mouse; D050697:Retinal Dehydrogenase; D014568:Urokinase-Type Plasminogen Activator