title: Membrane-active action mode of polybia-CP, a novel antimicrobial peptide isolated from the venom of Polybia paulista.
abstract: The extensive use of antibiotics in medicine, the food industry, and agriculture has resulted in the frequent emergence of multidrug-resistant bacteria, which creates an urgent need for new antibiotics. It is now widely recognized that antimicrobial peptides (AMPs) could play a promising role in fighting multidrug-resistant bacteria. Antimicrobial peptide polybia-CP was purified from the venom of the social wasp Polybia paulista. In this study, we synthesized polybia-CP and studied its action mode of antibacterial activity. Our results revealed that polybia-CP has potent antibacterial activity against both Gram-positive and Gram-negative bacteria. The results from both the real bacterial membrane and the in vitro model membrane showed that polybia-CP is membrane active and that its action target is the membrane of bacteria. It is difficult for bacteria to develop resistance to polybia-CP, which may thus offer a new strategy for defending against resistant bacteria in medicine and the food and farming industries.
authors.affiliation: Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences, Institute of Biochemistry and Molecular Biology, School of Life Sciences, Lanzhou University, Lanzhou, People's Republic of China.
authors.forename: Kairong
authors.initials: K
authors.lastname: Wang
authors.forename: Jiexi
authors.initials: J
authors.lastname: Yan
authors.forename: Ru
authors.initials: R
authors.lastname: Chen
authors.forename: Wen
authors.initials: W
authors.lastname: Dang
authors.forename: Bangzhi
authors.initials: B
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Jingjing
authors.initials: J
authors.lastname: Song
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D014688:Venoms / Q000737:chemistry*; D014863:Wasps / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D010455:Peptides; D014688:Venoms