title: [Research status and prospects of DNA test on difficult specimens].
abstract: This paper reviews the advances of DNA detection on three types of difficult biological specimens including degraded samples, trace evidences and mixed samples. The source of different samples, processing methods and announcements were analyzed. New methods such as mitochondrial test system, changing the original experimental conditions, low-volume PCR amplification and new technologies such as whole genome amplification techniques, laser capture micro-dissection, and mini-STR technology in recent years are introduced.
authors.affiliation: Institute of Forensic Science, Chengdu Public Security Bureau, Chengdu 610081, China. danghuawei@yahoo.cn
authors.forename: Hua-Wei
authors.initials: HW
authors.lastname: Dang
authors.forename: Jiong
authors.initials: J
authors.lastname: Mao
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Jiang-Ping
authors.initials: JP
authors.lastname: Huang
authors.forename: Xiao-Gang
authors.initials: XG
authors.lastname: Bai
mesh_terms: D015415:Biomarkers; D001826:Body Fluids / Q000737:chemistry; D004247:DNA / Q000032:analysis* / Q000235:genetics; D016172:DNA Fingerprinting / Q000379:methods*; D004272:DNA, Mitochondrial / Q000032:analysis / Q000235:genetics; D005554:Forensic Medicine / Q000379:methods*; D015894:Genome, Human; D006801:Humans; D061346:Laser Capture Microdissection / Q000379:methods; D018895:Microsatellite Repeats; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D018401:Sample Size
chemical_list: D015415:Biomarkers; D004272:DNA, Mitochondrial; D004247:DNA