title: Large-scale production of membrane proteins in Saccharomyces cerevisiae: using a green fluorescent protein fusion strategy in the production of membrane proteins.
abstract: The production of membrane proteins in the large quantities necessary for structural analysis requires many optimization steps. The GFP-fusion-based scheme described in earlier chapters (                 Chapters 4                                ,                  8                                , and                  16                                ) facilitates these steps by allowing the selection of high yielding clones that produce detergent-stable membrane proteins. Here, we describe the experimental steps required to establish the reproducible, large-scale production and purification of membrane protein-GFP fusions using S. cerevisiae.
authors.affiliation: Division of Molecular Biosciences, Membrane Protein Crystallography Group, Imperial College, London, UK.
authors.forename: David
authors.initials: D
authors.lastname: Drew
authors.forename: Hyun
authors.initials: H
authors.lastname: Kim
mesh_terms: D002850:Chromatography, Gel; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*
chemical_list: D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins