title: Two new cytotoxic triterpenoid saponins from the roots of Clematis argentilucida.
abstract: Bioassay-guided fractionation of the n-BuOH extract of the roots of Clematis argentilucida led to the isolation of two new triterpenoid saponins along with a known one, cussonside B (3). By extensive spectral analysis and chemical evidences, the structures of the two new saponins were determined to be 3β-O-[β-D-ribopyranosyl-(1→3)-α-L-rhamnopyranosyl-(1→2)-α-L-arabinopyranosyl] hederagenin-11,13-dien-28-oic acid (1) and 3β-O-{β-D-ribopyranosyl-(1→3)-α-L-rhamnopyranosyl-(1→2)-[β-D-glucopyranosyl-(1→4)]-β-D-xylopyranosyl} oleanolic acid (2), respectively. Saponin 1 is the first example of triterpenoid saponins with two double bonds located at C-11 and C-13 in the aglycone from the genus Clematis. The two new saponins exhibited significant cytotoxicity against human leukemia HL-60 cell lines, human hepatocellular carcinoma Hep-G2 cell lines and human glioblastoma U251MG cell lines with a range of IC(50) values from 2.74 to 25.40μM, while 3 showed inactivity against all of the three cancer cell lines.
authors.affiliation: Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, PR China.
authors.forename: Wenli
authors.initials: W
authors.lastname: Hai
authors.forename: Hua
authors.initials: H
authors.lastname: Cheng
authors.forename: Mei
authors.initials: M
authors.lastname: Zhao
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.forename: Liangjian
authors.initials: L
authors.lastname: Hong
authors.forename: Haifeng
authors.initials: H
authors.lastname: Tang
authors.forename: Xiangrong
authors.initials: X
authors.lastname: Tian
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D045744:Cell Line, Tumor; D039324:Clematis / Q000737:chemistry*; D018922:HL-60 Cells; D056945:Hep G2 Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D015394:Molecular Structure; D009369:Neoplasms / Q000188:drug therapy*; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D018517:Plant Roots / Q000737:chemistry; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: C575127:3beta-O-(beta-D-ribopyranosyl-(1-3)-alpha-L-rhamnopyranosyl-(1-2)-(beta-D-glucopyranosyl-(1-4))-beta-D-xylopyranosyl) oleanolic acid; C575126:3beta-O-(beta-D-ribopyranosyl-(1-3)-alpha-L-rhamnopyranosyl-(1-2)-alpha-L-arabinopyranosyl) hederagenin-11,13-dien-28-oic acid; D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D012503:Saponins; D014315:Triterpenes