title: A universal expression tag for structural and functional studies of proteins.
abstract: Modified ubiquitin sequences, each completed with a His tag and a TEV cleavage site, were designed to enhance the expression of protein/peptide targets. With this new system we have been able to characterize several peptide-protein interactions by ITC and by NMR and CD spectroscopic methods, including the interactions of LIR domains with autophagy modifiers.
authors.affiliation: Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Strasse 9, 60438 Frankfurt, Germany. rogov@bpc.uni-frankfurt.de
authors.forename: Vladimir V
authors.initials: VV
authors.lastname: Rogov
authors.forename: Alexis
authors.initials: A
authors.lastname: Rozenknop
authors.forename: Natalia Yu
authors.initials: NY
authors.lastname: Rogova
authors.forename: Frank
authors.initials: F
authors.lastname: Löhr
authors.forename: Suhas
authors.initials: S
authors.lastname: Tikole
authors.forename: Victor
authors.initials: V
authors.lastname: Jaravine
authors.forename: Peter
authors.initials: P
authors.lastname: Güntert
authors.forename: Ivan
authors.initials: I
authors.lastname: Dikic
authors.forename: Volker
authors.initials: V
authors.lastname: Dötsch
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D020224:Expressed Sequence Tags / Q000737:chemistry*; D005822:Genetic Vectors / Q000235:genetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D014176:Protein Biosynthesis; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry* / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics; D025801:Ubiquitin / Q000235:genetics / Q000378:metabolism
chemical_list: D011506:Proteins; D011993:Recombinant Fusion Proteins; D025801:Ubiquitin