title: Two-dimensional protein separation by the HPLC system with a monolithic column.
abstract: A two-dimensional high-performance liquid chromatography (2D-HPLC) system for protein separation was developed using an ion-exchange column in the first dimension and a reversed-phase monolithic column in the second dimension. The system demonstrated efficient separation of proteins in comparison with conventional systems. For proteomic analysis, proteins extracted from the cell surface of the yeast were separated by 2D-HPLC and evaluated.
authors.affiliation: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, Japan.
authors.forename: Hironobu
authors.initials: H
authors.lastname: Morisaka
authors.forename: Aya
authors.initials: A
authors.lastname: Kirino
authors.forename: Kengo
authors.initials: K
authors.lastname: Kobayashi
authors.forename: Mitsuyoshi
authors.initials: M
authors.lastname: Ueda
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange; D040901:Proteomics; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D029701:Saccharomyces cerevisiae Proteins