title: Isolation, modulatory functions on murine B cell development and antigen-specific immune responses of BP11, a novel peptide from the chicken bursa of Fabricius.
abstract: The bursa of Fabricius (BF) is the central humoral immune organ unique to birds which plays important roles in B lymphocyte differentiation. Here, a new bursal peptide (BP11) with the amino acid sequence DVAGKLPDNRT was identified and characterized from BF. It was proved that BP11 promoted CFU pre-B formation, and regulated B cell differentiation, including increase the percentage of immature and mature B cells in BM cells co-cultured with IL-7. BP11 also exerted immunomodulatory function on antigen-specific immune responses in BALB/c mice immunized with inactivated influence virus (AIV, H9N2 subtype) vaccine, including enhancing AIV-specific antibody and cytokine production. Furthermore, it was noteworthy that BP11 stimulated antibody productions and potentiates the Th1 and Th2-type immune responses in dose-dependent manner in chicken. These results suggested that BP11 might be highly relevant for the development of avian immune system.
authors.affiliation: Division of Key Lab of Animal Disease Diagnosis and Immunology of China's Department of Agriculture, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Liu
authors.forename: Xiu-Li
authors.initials: XL
authors.lastname: Feng
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.forename: Rui-Bing
authors.initials: RB
authors.lastname: Cao
authors.forename: Xin-Feng
authors.initials: XF
authors.lastname: Li
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Ma
authors.forename: Pu-Yan
authors.initials: PY
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D030161:Avian Proteins / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology*; D001402:B-Lymphocytes / Q000276:immunology / Q000502:physiology*; D002060:Bursa of Fabricius / Q000276:immunology* / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D002645:Chickens / Q000276:immunology*; D005260:Female; D007074:Immunoglobulin G / Q000097:blood; D007155:Immunologic Factors / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology*; D053127:Influenza A Virus, H9N2 Subtype / Q000276:immunology*; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009842:Oligopeptides / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology*
chemical_list: D000914:Antibodies, Viral; D030161:Avian Proteins; D007074:Immunoglobulin G; D007155:Immunologic Factors; D007252:Influenza Vaccines; D009842:Oligopeptides