title: Characterization of FimA in Porphyromonas gingivalis genotype IV.
abstract: It has been reported that a large majority of periodontitis patients carry organisms with either type II or IV-fimA, while type I is the most prevalent fimA genotype among Porphyromonas gingivalis-positive healthy adults. Here we report characterization of recombinant fimbrial protein (rFimA) produced in Escherichia coli from genotype IV-fimA. In SDS-PAGE and immunoblot analysis after partial dissociation, type IV-rFimA showed a ladder-like pattern representing oligomeric/polymeric forms of native fimbrial structure. Unlike anti-type I-native fimbriae which can only recognize conformational epitopes of the respective proteins, both anti-type IV-native fimbriae and anti-type IV-rFimA antibodies recognized conformational as well as linear epitopes in type IV-fimbriae. These results suggest that the type IV-rFimA proteins retain the native fimbrial antigenicity and the antigenicity of type IV-fimbriae is different from that of type I-fimbriae.
authors.affiliation: Department of Maxillofacial Biomedical Engineering, School of Dentistry, Kyung Hee University, Seoul, Korea.
authors.forename: Young-Suk
authors.initials: YS
authors.lastname: Choi
authors.forename: Ji-Hoi
authors.initials: JH
authors.lastname: Moon
authors.forename: Jae-Hong
authors.initials: JH
authors.lastname: Park
authors.forename: Jin-Yong
authors.initials: JY
authors.lastname: Lee
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli; D036781:Fimbriae Proteins / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D015151:Immunoblotting; D016966:Porphyromonas gingivalis / Q000737:chemistry / Q000235:genetics*; D011487:Protein Conformation; D055503:Protein Multimerization; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D011994:Recombinant Proteins; C063055:fimbrillin; D036781:Fimbriae Proteins