title: Rev-erbα regulates circadian rhythms and StAR expression in rat granulosa cells as identified by the agonist GSK4112.
abstract: The Rev-erbα gene is regarded as a circadian clock gene and clock-regulated gene which regulates the circadian transcriptional/translational loop in a subtle way. Here, we first detected the circadian oscillation in mature granulosa cells from antral follicles using a real-time monitoring system of Per2 promoter activity with the addition of FSH. Then we used GSK4112, an agonist ligand of Rev-erbα, to investigate the function of Rev-erbα. GSK4112 treatment significantly reduced the Per2-dLuc amplitude and induced the Per2 oscillation phase advance shift. GSK4112 significantly inhibited Bmal1 mRNA expression, whereas it did clearly stimulate expression of StAR mRNA in a dose-dependent manner. Our data are the first to show the Rev-erbα function in the steroid biosynthesis of rat granulosa cells, and to suggest that Rev-erbα may coordinate circadian rhythm and metabolism in rat ovaries.
authors.affiliation: Department of Animal and Marine Bioresource Sciences, Graduate School of Agriculture, Kyushu University, Fukuoka, Japan.
authors.forename: Huatao
authors.initials: H
authors.lastname: Chen
authors.forename: Guiyan
authors.initials: G
authors.lastname: Chu
authors.forename: Lijia
authors.initials: L
authors.lastname: Zhao
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Yamauchi
authors.forename: Yasufumi
authors.initials: Y
authors.lastname: Shigeyoshi
authors.forename: Seiichi
authors.initials: S
authors.lastname: Hashimoto
authors.forename: Masa-aki
authors.initials: MA
authors.lastname: Hattori
mesh_terms: D056930:ARNTL Transcription Factors / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D000818:Animals; D002940:Circadian Rhythm / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation; D005998:Glycine / Q000031:analogs & derivatives / Q000494:pharmacology; D006107:Granulosa Cells / Q000378:metabolism / Q000502:physiology*; D056953:Nuclear Receptor Subfamily 1, Group D, Member 1 / Q000819:agonists / Q000378:metabolism*; D056950:Period Circadian Proteins / Q000235:genetics / Q000378:metabolism; D010750:Phosphoproteins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D051381:Rats; D013876:Thiophenes / Q000494:pharmacology; D000098682:Steroidogenic Acute Regulatory Protein
chemical_list: D056930:ARNTL Transcription Factors; C572356:GSK4112; C498263:Nr1d1 protein, rat; D056953:Nuclear Receptor Subfamily 1, Group D, Member 1; C513311:Per2 protein, rat; D056950:Period Circadian Proteins; D010750:Phosphoproteins; D013876:Thiophenes; D000098682:Steroidogenic Acute Regulatory Protein; D005998:Glycine