title: Community structure analysis of methanogens associated with rumen protozoa reveals bias in universal archaeal primers.
abstract: The diversity of protozoan-associated methanogens in cattle was investigated using five universal archaeal small-subunit (SSU) rRNA gene primer sets. Methanobrevibacter spp. and rumen cluster C (distantly related to Thermoplasma spp.) were predominant. Significant differences in species composition among libraries indicate that some primers used previously to characterize rumen methanogens exhibit biased amplification.
authors.affiliation: Agriculture and Agri-Food Canada, Lethbridge, Alberta, Canada. lisa.tymensen@gov.ab.ca
authors.forename: Lisa D
authors.initials: LD
authors.lastname: Tymensen
authors.forename: Tim A
authors.initials: TA
authors.lastname: McAllister
mesh_terms: D000818:Animals; D001105:Archaea / Q000145:classification / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification*; D002417:Cattle; D017931:DNA Primers; D017753:Ecosystem; D041581:Methanobrevibacter / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics; D012417:Rumen / Q000382:microbiology* / Q000469:parasitology*; D017422:Sequence Analysis, DNA; D013822:Thermoplasma / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification
chemical_list: D017931:DNA Primers; D012335:RNA, Ribosomal