title: Evaluation of the relationship between leptin, resistin, adiponectin and natural regulatory T cells in relapsing-remitting multiple sclerosis.
abstract: OBJECTIVE
  Data suggest that adipocytokines and natural regulatory T (nTreg) cells play a pivotal role in the immunopathogenesis of multiple sclerosis and the associated inflammation. The purpose of this study was to evaluate selected adipocytokines and nTreg cells and to assess their relationship with relapsing-remitting multiple sclerosis (RRMS).
  
  
  METHODS
  The study was conducted among 25 patients with RRMS and 25 healthy individuals. Blood samples were collected within two weeks after the beginning of acute relapse of RRMS. The body mass index (BMI) of each patient was calculated. Serum adipocytokine concentrations were determined by ELISA and nTreg cells were evaluated using multicolour flow cytometry.
  
  
  RESULTS
  Patients and controls had similar BMI, regardless of gender. Significantly higher leptin and resistin levels and significantly lower adiponectin levels were found in patients with RRMS in comparison to the control group (p < 0.0001). The percentage of nTreg cells (p < 0.01) and the mean fluorescence channel (MFC) of FoxP3 were significantly reduced in patients with RRMS (p < 0.001). There was an inverse correlation be-tween leptin concentration and MFC of the transcription factor Foxp3 nTreg in patients with RRMS (r = -0.7, p < 0.05).
  
  
  CONCLUSIONS
  Proinflammatory adipocytokine profile and decreased percentage of nTreg cells suggest their implication in the inflammatory response in RRMS regardless of corticosteroid therapy. The correlation between leptin and the MFC of the transcription factor Foxp3 in nTreg cells in patients with RRMS suggests its inhibitory effect on FoxP3 expression.
authors.affiliation: Zakład Diagnostyki Laboratoryjnej, II Katedra Wewnetrznych, Uniwersytet Medyczny w Łódź, Poland. lukasz.kraszula@umed.lodz.pl
authors.forename: Lukasz
authors.initials: L
authors.lastname: Kraszula
authors.forename: Anna
authors.initials: A
authors.lastname: Jasińska
authors.forename: Makandjou-Ola
authors.initials: M
authors.lastname: Eusebio
authors.forename: Piotr
authors.initials: P
authors.lastname: Kuna
authors.forename: Andrzej
authors.initials: A
authors.lastname: Głąbiński
authors.forename: Mirosława
authors.initials: M
authors.lastname: Pietruczuk
mesh_terms: D052242:Adiponectin / Q000097:blood*; D000328:Adult; D015415:Biomarkers / Q000097:blood; D015992:Body Mass Index; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D020738:Leptin / Q000097:blood*; D008297:Male; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000097:blood* / Q000276:immunology*; D011044:Poland; D052243:Resistin / Q000097:blood*; D050378:T-Lymphocytes, Regulatory / Q000378:metabolism*; D055815:Young Adult
chemical_list: D052242:Adiponectin; D015415:Biomarkers; D020738:Leptin; D052243:Resistin