title: Surface-expressed enolase contributes to the adhesion of Paracoccidioides brasiliensis to host cells.
abstract: Paracoccidioidomycosis is a systemic mycosis caused by the dimorphic fungus Paracoccidioides brasiliensis. Understanding the interactions between P. brasiliensis and the host tissue depends on the study of the different steps of the process of colonization, especially adhesion, in which the pathogen recognizes ligands on the surface of host cells. This study aimed to verify the role of enolase in the host cell-fungus interaction and the ability of enolase to bind to extracellular matrix components, to determine its subcellular localization, and to study the P. brasiliensis enolase amino acid sequence. The data revealed that fibronectin is the major ligand of enolase. Evaluation of the location of enolase at an ultrastructural level revealed that it is distributed in various cellular compartments, but at a high level in the cell wall. The analysis of the amino acid sequence revealed an internal plasminogen-binding motif ((254)FYKADEKKY(262)), which is conserved in most organisms and described as an important interaction site of the enolase with the host cell surface. This suggests that enolase performs additional functions related to the glycolytic pathway and also plays a role of adhesion in P. brasiliensis. Therefore, this study increases the knowledge about the characteristics of enolase and its influence on the binding process of P. brasiliensis.
authors.affiliation: Faculty of Pharmaceutical Sciences, UNESP - Univ Estadual Paulista Araraquara, Department of Clinical Analysis, Clinical Mycology Laboratory, SP, Brazil.
authors.forename: Caroline Maria
authors.initials: CM
authors.lastname: Marcos
authors.forename: Julhiany
authors.initials: J
authors.lastname: de Fátima da Silva
authors.forename: Haroldo Cesar
authors.initials: HC
authors.lastname: de Oliveira
authors.forename: Rosângela Aparecida
authors.initials: RA
authors.lastname: Moraes da Silva
authors.forename: Maria José Soares
authors.initials: MJ
authors.lastname: Mendes-Giannini
authors.forename: Ana Marisa
authors.initials: AM
authors.lastname: Fusco-Almeida
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D002448:Cell Adhesion; D002473:Cell Wall / Q000201:enzymology; D005353:Fibronectins / Q000378:metabolism; D054884:Host-Pathogen Interactions; D006801:Humans; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D010228:Paracoccidioides / Q000201:enzymology* / Q000472:pathogenicity*; D010751:Phosphopyruvate Hydratase / Q000378:metabolism*
chemical_list: D005353:Fibronectins; D010751:Phosphopyruvate Hydratase