title: Determination of macrolide antibiotics using dispersive liquid-liquid microextraction followed by surface-assisted laser desorption/ionization mass spectrometry.
abstract: A novel method for the determination of macrolide antibiotics using dispersive liquid-liquid microextraction coupled to surface-assisted laser desorption/ionization mass spectrometric detection was developed. Acetone and dichloromethane were used as the disperser solvent and extraction solvent, respectively. A mixture of extraction solvent and disperser solvent were rapidly injected into a 1.0 mL aqueous sample to form a cloudy solution. After the extraction, macrolide antibiotics were detected using surface-assisted laser desorption/ionization mass spectrometry (SALDI/MS) with colloidal silver as the matrix. Under optimum conditions, the limits of detection (LODs) at a signal-to-noise ratio of 3 were 2, 3, 3, and 2 nM for erythromycin (ERY), spiramycin (SPI), tilmicosin (TILM), and tylosin (TYL), respectively. This developed method was successfully applied to the determination of macrolide antibiotics in human urine samples.
authors.affiliation: Department of Chemistry, National Kaohsiung Normal University, no. 62, Shenjhong Rd., Yanchao, Kaohsiung, Taiwan, 824.
authors.forename: Kuan-Yu
authors.initials: KY
authors.lastname: Chen
authors.forename: Thomas C
authors.initials: TC
authors.lastname: Yang
authors.forename: Sarah Y
authors.initials: SY
authors.lastname: Chang
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification / Q000652:urine; D006801:Humans; D057230:Limit of Detection; D059627:Liquid Phase Microextraction / Q000379:methods*; D018942:Macrolides / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification / Q000652:urine; D059629:Signal-To-Noise Ratio; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents; D018942:Macrolides