title: Effect of the application of a casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) paste and adhesive systems on bond durability of a fissure sealant.
abstract: This study aimed to evaluate the effect of the previous application of a casein phosphopeptide-amorphous calcium phosphate paste (MI Paste, MI) and adhesive systems on the bond durability of a fissure sealant. Ninety-eight enamel blocks were obtained from proximal surfaces of erupted third molars. Specimens were divided into 14 groups (n = 7) according to the previous application of MI (with and without) and the adhesive systems used (no adhesive system; hydrophobic resin of a three-step etch-and-rinse adhesive system; etch-and-rinse single-bottle adhesive system; all-in-one adhesive system; two-step self-etching adhesive system; additional phosphoric acid conditioning and all-in-one adhesive system; additional phosphoric acid conditioning and two-step self-etching adhesive system). A fissure sealant (Fluroshield) was applied and photoactivated for 20 s. Beams (~0.7 mm(2)) were prepared for the microtensile bond strength test, which was executed after 24 h or 6 months of water storage. Fractured specimens were analyzed by scanning electronic microscopy. Data were analyzed by two-way ANOVA with repeated measures/Tukey's test (P < 0.05). Groups that received MI application and adhesive systems presented higher means than those groups where MI was not applied. Higher frequency of cohesive failures was observed for groups with MI. Applying a CPP-ACP containing paste on enamel before adhesive systems was an effective method to increase bond durability of the sealant tested.
authors.affiliation: Department of Dentistry, School of Dentistry, Potiguar University (Laureate International Universities), Av Senador Salgado Filho 1610, Natal, Brazil. boniek.castillo@gmail.com
authors.forename: Boniek Castillo Dutra
authors.initials: BC
authors.lastname: Borges
authors.forename: Anderson
authors.initials: A
authors.lastname: Catelan
authors.forename: Robson Tetsuo
authors.initials: RT
authors.lastname: Sasaki
authors.forename: Gláucia Maria Bovi
authors.initials: GM
authors.lastname: Ambrosano
authors.forename: André Figueiredo
authors.initials: AF
authors.lastname: Reis
authors.forename: Flávio Henrique Baggio
authors.initials: FH
authors.lastname: Aguiar
mesh_terms: D000704:Analysis of Variance; D002364:Caseins / Q000737:chemistry*; D001840:Dental Bonding / Q000379:methods*; D003738:Dental Cements / Q000737:chemistry*; D003743:Dental Enamel / Q000187:drug effects*; D045662:Dental Etching / Q000379:methods; D019232:Dental Restoration Failure; D006801:Humans; D008964:Molar, Third; D010895:Pit and Fissure Sealants / Q000737:chemistry*; D013718:Tensile Strength
chemical_list: D002364:Caseins; D003738:Dental Cements; D010895:Pit and Fissure Sealants; C447032:casein phosphopeptide-amorphous calcium phosphate nanocomplex