title: [Detection of epidermal growth factor receptor gene mutation in non-small cell lung cancer by allele-specific oligonucleotide-PCR and bi-loop probe specific primer quantitative PCR].
abstract: OBJECTIVE
  To compare the detection sensitivity of epidermal growth factor receptor (EGFR) mutations between allele specific oligonucleotide PCR (ASO-PCR) and bi-loop probe and specific primer quantitative PCR (BPSP-qPCR).
  
  
  METHODS
  A total of 96 non-small cell lung cancer specimens were selected from West China Hospital from September 2009 to December 2010. ASO-PCR was developed to detect the presence of classical EGFR mutations. A total 39 available specimens were also tested by BPSP-qPCR.
  
  
  RESULTS
  EGFR mutation detection rate was 30.2% (26/96) by ASO-PCR. The mutation rate was higher in female than in male patients [45.5% (20/44) vs. 17.3% (9/52), P = 0.003], non-smokers than smokers [44.1% (26/59) vs. 8.1% (3/37), P < 0.001] and adenocarcinomas than other subtypes of lung cancer [37.0% (27/73) vs. 8.7% (2/23), P = 0.01]. Among mutation negative cases by ASO-PCR, BPSP-qPCR increased the rate of detection of 19-del and L858R mutation by 10.3% (4/39) in adenocarcinomas and non-smoking subset. Overall, the mutation detection rate of BPSP-qPCR was higher than that of ASO-PCR [66.7% (26/39) vs. 41.0% (16/39), P = 0.02].
  
  
  CONCLUSIONS
  BPSP-qPCR has a better detection sensitivity than that of ASO-PCR.
authors.affiliation: Department of Thoracic Oncology, Cancer Center, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Deng
authors.forename: Yuan
authors.initials: Y
authors.lastname: Tang
authors.forename: Jing
authors.initials: J
authors.lastname: Lin
authors.forename: Xiao-jun
authors.initials: XJ
authors.lastname: Lu
authors.forename: Jian-xin
authors.initials: JX
authors.lastname: Xue
authors.forename: Li-shuai
authors.initials: LS
authors.lastname: Wang
authors.forename: Lin
authors.initials: L
authors.lastname: Zhou
authors.forename: Yan
authors.initials: Y
authors.lastname: Zou
authors.forename: Bin-wu
authors.initials: BW
authors.lastname: Ying
authors.forename: Gan-di
authors.initials: GD
authors.lastname: Li
authors.forename: You
authors.initials: Y
authors.lastname: Lu
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D004252:DNA Mutational Analysis; D066246:ErbB Receptors / Q000235:genetics*; D005260:Female; D018773:Genes, erbB-1; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D012737:Sex Factors; D012907:Smoking
chemical_list: C512478:EGFR protein, human; D066246:ErbB Receptors