title: Up-regulation of Anxa2 gene promotes proliferation and invasion of breast cancer MCF-7 cells.
abstract: OBJECTIVE
  The metastatic ability of breast cancer cells with chemoresistant properties is higher when compared to that of their parental wild-type cells. Expression of AnnexinA2 (Anxa2), a 36-kDa calcium-dependent phospholipid binding protein, is increased in metastatic tumours and has been found to be associated with the phenotype of drug resistance and metastasis.
  
  
  RESULTS
  In the present study, we found that up-regulation of Anxa2 correlates with enhanced migration and invasion ability of MCF-7 breast cancer cells both in vitro and in vivo. Western blot analysis revealed that exposure to chemotherapeutic drugs may induce elevated expression of Anxa2. In addition, our data have shown that Anxa2 might influence proliferation, migration and invasion of MCF-7 cells by increasing expression of c-myc and cyclin D1 via activation of Erk1/2 signalling pathways.
  
  
  CONCLUSIONS
  Our findings suggest that up-regulation of Anxa2 may play an important role in modulating proliferation and invasion of breast cancer MCF-7 cells through regulation of many relevant downstream target genes.
authors.affiliation: Key Laboratory of Breast Cancer Prevention and Therapy, Ministry of Education, Tianjin Medical University Cancer Institute and Hospital, Tianjin Medical University, Tianjin, China.
authors.forename: B
authors.initials: B
authors.lastname: Wu
authors.forename: F
authors.initials: F
authors.lastname: Zhang
authors.forename: M
authors.initials: M
authors.lastname: Yu
authors.forename: P
authors.initials: P
authors.lastname: Zhao
authors.forename: W
authors.initials: W
authors.lastname: Ji
authors.forename: H
authors.initials: H
authors.lastname: Zhang
authors.forename: J
authors.initials: J
authors.lastname: Han
authors.forename: R
authors.initials: R
authors.lastname: Niu
mesh_terms: D000818:Animals; D017306:Annexin A2 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D019938:Cyclin D1 / Q000378:metabolism; D019008:Drug Resistance, Neoplasm; D005260:Female; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D009361:Neoplasm Invasiveness; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D016196:S Phase; D050796:STAT3 Transcription Factor / Q000378:metabolism; D015398:Signal Transduction; D014183:Transplantation, Heterologous; D015854:Up-Regulation
chemical_list: D017306:Annexin A2; D016271:Proto-Oncogene Proteins c-myc; D034741:RNA, Small Interfering; D050796:STAT3 Transcription Factor; D019938:Cyclin D1; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3