title: Ycf1p attenuates basal level oxidative stress response in Saccharomyces cerevisiae.
abstract: Ycf1p function is regulated by casein kinase 2α, Cka1p, via phosphorylation of Ser251. Cka1p-mediated phosphorylation of Ycf1p is attenuated in response to high salt stress. Previous results from our lab suggest a role for Ycf1p in cellular resistance to salt stress. Here, we show that Ycf1p plays an important role in cellular resistance to salt stress by maintaining the cellular redox balance via glutathione recycling. Our results suggest that during acute salt stress increased Sod1p, Sod2p and Ctt1p activity is the main compensatory for the loss in Ycf1p function that results from reduced Ycf1p-dependent recycling of cellular GSH levels.
authors.affiliation: Department of Toxicology, University of Kentucky, Lexington, KY 40536, USA. cmpaum2@uky.edu
authors.forename: Christian M
authors.initials: CM
authors.lastname: Paumi
authors.forename: Kerry A
authors.initials: KA
authors.lastname: Pickin
authors.forename: Roaa
authors.initials: R
authors.lastname: Jarrar
authors.forename: Catherine K
authors.initials: CK
authors.lastname: Herren
authors.forename: Stuart T
authors.initials: ST
authors.lastname: Cowley
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D047390:Casein Kinase II / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology; D016877:Oxidants / Q000494:pharmacology; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010766:Phosphorylation; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D012492:Salts / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D000072105:Superoxide Dismutase-1; D000099156:Superoxide Dismutase 2
chemical_list: D018528:ATP-Binding Cassette Transporters; D015415:Biomarkers; D047390:Casein Kinase II; D005978:Glutathione; D006861:Hydrogen Peroxide; D016877:Oxidants; D029701:Saccharomyces cerevisiae Proteins; D012492:Salts; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D000099156:Superoxide Dismutase 2; C089179:YCF1 protein, S cerevisiae