title: Markerless gene deletion system for sphingomonads.
abstract: Here, we suggest that natural streptomycin resistance of many sphingomonads resides within rpsL. We constructed a dominant, streptomycin-sensitive rpsL allele and demonstrated its use as a counterselection marker in several sphingomonads. An rpsL-based markerless gene deletion system was developed and validated by deleting four genes in Sphingomonas sp. strain Fr1.
authors.affiliation: Institute of Microbiology, ETH Zurich, Zurich, Switzerland.
authors.forename: Andreas
authors.initials: A
authors.lastname: Kaczmarczyk
authors.forename: Julia A
authors.initials: JA
authors.lastname: Vorholt
authors.forename: Anne
authors.initials: A
authors.lastname: Francez-Charlot
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D058448:Biota; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D024881:Drug Resistance, Bacterial; D017353:Gene Deletion; D005827:Genetics, Microbial / Q000379:methods*; D005838:Genotype; D008969:Molecular Sequence Data; D012269:Ribosomal Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D020578:Sphingomonas / Q000187:drug effects / Q000235:genetics*; D013307:Streptomycin / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D012269:Ribosomal Proteins; D013307:Streptomycin