title: Adaptive synthetic-aperture focusing technique for microvasculature imaging using photoacoustic microscopy.
abstract: To improve the lateral resolution of the blood vessels along arbitrary direction out of focus in photoacoustic microscopy (PAM), we propose an adaptive synthetic-aperture focusing technique (ASAFT) for microvasculature imaging which can be automatically applied to each branch of blood vessels, based on our previous two-dimensional (2D) SAFT. The ASAFT is validated both in the phantom study and in vivo imaging. The results demonstrate that ASAFT can provide images of blood vessels with better lateral resolution both at different depths and along various directions compared with one-dimensional and 2D SAFT.
authors.affiliation: Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong, University of Science and Technology, 1037 Luoyu Road, Wuhan 430074, China.
authors.forename: Zilin
authors.initials: Z
authors.lastname: Deng
authors.forename: Xiaoquan
authors.initials: X
authors.lastname: Yang
authors.forename: Hui
authors.initials: H
authors.lastname: Gong
authors.forename: Qingming
authors.initials: Q
authors.lastname: Luo
mesh_terms: D000818:Animals; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005246:Feedback; D007089:Image Enhancement / Q000295:instrumentation*; D007909:Lenses; D051379:Mice; D008807:Mice, Inbred BALB C; D033401:Microscopy, Acoustic / Q000295:instrumentation*; D055806:Microvessels / Q000033:anatomy & histology* / Q000000981:diagnostic imaging*; D061088:Photoacoustic Techniques / Q000295:instrumentation*