title: Increasing throughput of surface plasmon resonance-based biosensors by multiple analyte injections.
abstract: Surface plasmon resonance-based biosensors are now acknowledged as robust and reliable instruments to determine the kinetic parameters related to the interactions between biomolecules. These kinetic parameters are used in screening campaigns: there is a considerable interest in reducing the experimental time, thus improving the throughput of the surface plasmon resonance assays. Kinetic parameters are typically obtained by analyzing data from several injections of a given analyte at different concentrations over a surface where its binding partner has been immobilized. It has been already proven that an iterative optimization approach aiming at determining optimal analyte injections to be performed online can significantly reduce the experimentation time devoted to kinetic parameter determination, without any detrimental effect on their standard errors. In this study, we explore the potential of this iterative optimization approach to further reduce experiment duration by combining it with the simultaneous injection of two analytes.
authors.affiliation: Department of Chemical Engineering, École Polytechnique de Montréal, PO Box 6079, Centre-ville Station, Montréal, Québec, Canada H3C 3A7.
authors.forename: Massinissa Si
authors.initials: MS
authors.lastname: Mehand
authors.forename: Gregory
authors.initials: G
authors.lastname: De Crescenzo
authors.forename: Bala
authors.initials: B
authors.lastname: Srinivasan
mesh_terms: D000465:Algorithms; D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D024402:Carbonic Anhydrase II / Q000737:chemistry; D002417:Cattle; D007700:Kinetics; D008958:Models, Molecular; D011485:Protein Binding; D000077145:Sulfanilamide; D013424:Sulfanilamides / Q000737:chemistry; D013449:Sulfonamides / Q000737:chemistry; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D013424:Sulfanilamides; D013449:Sulfonamides; D000077145:Sulfanilamide; D024402:Carbonic Anhydrase II