title: Tracking of the viability of Stenotrophomonas maltophilia bacteria population in polyvinylalcohol nanofiber webs by positron annihilation lifetime spectroscopy.
abstract: Polyvinylalcohol (PVA) fiber web containing embedded bacteria was prepared by electrospinning technique. From the point of the complex functionality of such potential delivery systems, it will be of impact how bacteria can survive in such artificial biotopes. The present study suggests a possible fast method for the tracking of the viability of the embedded bacteria based on the changes of the supramolecular structure of the polymeric delivery system caused by the metabolic product of the bacteria. Positron annihilation lifetime spectroscopy (PALS) was applied to track the free volume changes of the system in the course of storage. The PALS method sensitively detected the free volume changes, thus the viability of the bacteria in the polymeric fiber web.
authors.affiliation: University Pharmacy Department of Pharmacy Administration, Semmelweis University, Hőgyes Endre Str. 7-9, H-1092 Budapest, Hungary.
authors.forename: Attila
authors.initials: A
authors.lastname: Vajdai
authors.forename: Barnabás
authors.initials: B
authors.lastname: Szabó
authors.forename: Károly
authors.initials: K
authors.lastname: Süvegh
authors.forename: Romána
authors.initials: R
authors.lastname: Zelkó
authors.forename: Gabriella
authors.initials: G
authors.lastname: Ujhelyi
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D004563:Electrochemistry / Q000379:methods; D057139:Nanofibers; D011108:Polymers / Q000737:chemistry; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D013057:Spectrum Analysis / Q000379:methods*; D020615:Stenotrophomonas maltophilia / Q000378:metabolism*; D013997:Time Factors
chemical_list: D001672:Biocompatible Materials; D011108:Polymers; D011142:Polyvinyl Alcohol