title: Emerging technology of in situ cell free expression protein microarrays.
abstract: Recently, in situ protein microarrays have been developed for large scale analysis and high throughput studies of proteins. In situ protein microarrays produce proteins directly on the solid surface from pre-arrayed DNA or RNA. The advances in in situ protein microarrays are exemplified by the ease of cDNA cloning and cell free protein expression. These technologies can evaluate, validate and monitor protein in a cost effective manner and address the issue of a high quality protein supply to use in the array. Here we review the importance of recently employed methods: PISA (protein in situ array), DAPA (DNA array to protein array), NAPPA (nucleic acid programmable protein array) and TUSTER microarrays and the role of these methods in proteomics.
authors.affiliation: National Center for Nanoscience and Technology, Chinese Academy of Sciences, Beijing, 100190, China.
authors.forename: Amita
authors.initials: A
authors.lastname: Nand
authors.forename: Anju
authors.initials: A
authors.lastname: Gautam
authors.forename: Javier Batista
authors.initials: JB
authors.lastname: Pérez
authors.forename: Alejandro
authors.initials: A
authors.lastname: Merino
authors.forename: Jinsong
authors.initials: J
authors.lastname: Zhu
mesh_terms: D002474:Cell-Free System; D004247:DNA / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D040081:Protein Array Analysis; D011506:Proteins / Q000378:metabolism*; D012313:RNA / Q000378:metabolism
chemical_list: D011506:Proteins; D012313:RNA; D004247:DNA