title: Lipases production by solid-state fermentation: the case of Rhizopus homothallicus in perlite.
abstract: Lipases are widely used in the industry for different purposes. Although these enzymes are mainly produced by submerged fermentation, lipase production by solid-state fermentation (SSF) has been gaining interest due to the advantages of this type of culture. Major advantages are higher production titers and productivity, less catabolite repression, and use of the dried fermented material as biocatalyst. This chapter describes a traditional methodology to produce fungal (Rhizopus homothallicus) lipases by SSF using perlite as inert support. The use of different devices (glass columns or Erlenmeyer flasks) and type of inoculum (spores or growing mycelium) is considered so that lipase production by SSF could be easily performed in any laboratory.
authors.affiliation: Departamento de Biotecnología, Universidad Autónoma Metropolitana-Iztapalapa, D.F. Mexico, Mexico.
authors.forename: Susana
authors.initials: S
authors.lastname: Velasco-Lozano
authors.forename: Tania
authors.initials: T
authors.lastname: Volke-Sepulveda
authors.forename: Ernesto
authors.initials: E
authors.lastname: Favela-Torres
mesh_terms: D000537:Aluminum Oxide / Q000737:chemistry; D019149:Bioreactors; D001709:Biotechnology; D015169:Colony Count, Microbial; D005285:Fermentation; D005898:Glass / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000096:biosynthesis*; D025282:Mycelium / Q000201:enzymology*; D012233:Rhizopus / Q000201:enzymology*; D012822:Silicon Dioxide / Q000737:chemistry; D013172:Spores, Fungal / Q000201:enzymology*
chemical_list: C003076:Perlite; D012822:Silicon Dioxide; D008049:Lipase; D000537:Aluminum Oxide