title: Effect of nalidixic acid on the morphology and protein expression of Pseudomonas aeruginosa.
abstract: OBJECTIVE
  To determine the effect of nalidixic acid on the morphology and protein expression of Pseudomonas aeruginosa (P. aeruginosa).
  
  
  METHODS
  Nalidixic acid solution of 1 600 μg/mL was prepared. The minimum inhibitory concentration (MIC) for P. aeruginosa was determined with tube dilution test. The effect of nalidixic acid on the morphology of P. aeruginosa was studied using light microscope and scanning electron microscope. Changes in protein profile were studied using SDS-PAGE.
  
  
  RESULTS
  The MIC of nalidixic acid was 700 μg/mL against P. aeruginosa. The exposure of P. aeruginosa to different concentrations of nalidixic acid resulted in deformation of most of the growing cells. At the concentration of 600 μg/mL most of the cells turned into elongated and adhere to each other while some of the cells were bulged. The intensity of protein bands were changed when they exposed to nalidixic acid.
  
  
  CONCLUSIONS
  The present findings suggest that the morphology and protein expression of P. aeruginosa is greatly affected by nalidixic acid.
authors.affiliation: Department of Biology, College of Science, P. O. Box: 36, P.C. 123, Sultan Qaboos University, Sultanate of Oman. snbahry@squ.edu.om
authors.forename: Saif
authors.initials: S
authors.lastname: Al Bahry
authors.forename: Nallusamy
authors.initials: N
authors.lastname: Sivakumar
authors.forename: Manal
authors.initials: M
authors.lastname: Al-Khambashi
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D009268:Nalidixic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure; D013053:Spectrophotometry
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D009268:Nalidixic Acid