title: Identification of new altered genes in rat cochleae with noise-induced hearing loss.
abstract: Because genes that are highly expressed in the cochlea after noise stress may have crucial regulatory roles in hearing, the identification of these genes may be useful for restoring normal auditory function. This study assessed altered gene expression at 1h following the cessation of noise exposure by using microarrays and real-time polymerase chain reaction (qPCR) in rats. In addition, the auditory threshold shifts and morphological changes of hair cells were observed. This study indicated that applied noise induced outer hair cell loss and a 40-50 dB hearing loss. Totally 239 altered genes were involved in the immune system process, response to stress, or response to stimulus. The expression of five up-regulated genes (Reg3b, Lcn2, Serpina3n, Nob1 and Hamp) was confirmed by qPCR. Future experiments will focus on several of these new candidate genes and may provide insight into the underlying auditory pathophysiology.
authors.affiliation: Department of Otolaryngology, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, Shaanxi Province, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Han
authors.forename: Liu
authors.initials: L
authors.lastname: Hong
authors.forename: Cuiping
authors.initials: C
authors.lastname: Zhong
authors.forename: Yang
authors.initials: Y
authors.lastname: Chen
authors.forename: Ye
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Mao
authors.forename: Daqing
authors.initials: D
authors.lastname: Zhao
authors.forename: Jianhua
authors.initials: J
authors.lastname: Qiu
mesh_terms: D000818:Animals; D003051:Cochlea / Q000378:metabolism*; D020869:Gene Expression Profiling; D006198:Hair Cells, Auditory / Q000378:metabolism; D006317:Hearing Loss, Noise-Induced / Q000235:genetics*; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction