title: Isolation of insulin-producing cells from different populations of multipotent stromal cells of the umbilical cord and human adipose tissue.
abstract: Stromal cells of adipose tissue and human umbilical cord were isolated by the original method from general populations of multipotent subpopulation of multipotent stromal cells exhibiting perivascular phenotype (CD146+, CD31-). Effective directed differentiation of these cells into insulin-producing cells by transient transfection of the gene Pdx1 was demonstrated. Transfection multipotent stromal cells CD146-, CD31- derived from adipose tissue and umbilical cord and isolated by the standard method, did not result in activation of insulin gene transcription. It was shown that the expression of nestin was not necessary for effective pancreatic cell differentiation.
authors.affiliation: Medical Genetics Research Center of Russian Academy of Medical Sciences, Moscow; ReMeTex Company, Moscow, Russia. irina_spirova@list.ru
authors.forename: I A
authors.initials: IA
authors.lastname: Fedyunina
authors.forename: A A
authors.initials: AA
authors.lastname: Rzhaninova
authors.forename: E E
authors.initials: EE
authors.lastname: Kirienko
authors.forename: D V
authors.initials: DV
authors.lastname: Goldshtein
mesh_terms: D000256:Adenoviridae; D000273:Adipose Tissue / Q000166:cytology*; D051929:CD146 Antigen / Q000032:analysis; D002454:Cell Differentiation; D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology*; D005434:Flow Cytometry; D015870:Gene Expression; D005947:Glucose / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000096:biosynthesis*; D050417:Insulin-Secreting Cells / Q000166:cytology* / Q000378:metabolism; D007381:Intermediate Filament Proteins / Q000235:genetics / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D064231:Nestin; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000032:analysis; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
chemical_list: D051929:CD146 Antigen; D018398:Homeodomain Proteins; D007328:Insulin; D007381:Intermediate Filament Proteins; C577484:NES protein, human; D009419:Nerve Tissue Proteins; D064231:Nestin; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D015534:Trans-Activators; C084010:pancreatic and duodenal homeobox 1 protein; D005947:Glucose