title: Preparation of water-soluble maleimide-functionalized 3 nm gold nanoparticles: a new bioconjugation template.
abstract: We present an efficient methodology to prepare maleimide-tethered, water-soluble gold nanoparticles (maleimide-AuNPs). The maleimide-AuNPs were prepared in the protected form and are readily recovered via a retro-Diels-Alder reaction. The maleimide-AuNPs were fully characterized by (1)H NMR, TGA, TEM, and XPS and were determined to have a gold core with an average size of 3.2 ± 0.8 nm; each core contains about 1000 gold atoms and is surrounded by 30 maleimide-terminated ligands and 60 thiolated PEG ligands. The maleimide-AuNPs efficiently react with rhodamine 123 and cysteine and are a promising template for biological applications.
authors.affiliation: Lady Davis Institute for Medical Research, Jewish General Hospital, McGill University, Montreal, QC, Canada.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhu
authors.forename: Carmen
authors.initials: C
authors.lastname: Waengler
authors.forename: R Bruce
authors.initials: RB
authors.lastname: Lennox
authors.forename: Ralf
authors.initials: R
authors.lastname: Schirrmacher
mesh_terms: D006046:Gold / Q000737:chemistry*; D008301:Maleimides / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D012995:Solubility; D014867:Water / Q000737:chemistry*
chemical_list: D008301:Maleimides; D014867:Water; C043592:maleimide; D006046:Gold