title: Virulence genes of Porphyromonas gingivalis W83 in chronic periodontitis.
abstract: OBJECTIVE
  To identify virulence genes found in highly virulent strains of Porphyromonas gingivalis (P. gingivalis) among Chinese patients with chronic periodontitis and to evaluate the association of these virulence genes with clinical parameters and with periodontal tissue destruction.
  
  
  METHODS
  Suppression subtractive hybridization was applied to acquire short gene fragments harbored only in virulent strains of P. gingivalis W83. Eighteen genes, which were present in P. gingivalis W83 but absent from P. gingivalis ATCC 33277, were labeled with Cy5 and used as probes in DNA microarray hybridization to analyze DNA of P. gingivalis isolated from chronic periodontitis patients.
  
  
  RESULTS
  Spearman correlation analysis revealed 10 genes correlated with probing depth, clinical attachment loss, and tooth mobility (p<0.05).
  
  
  CONCLUSIONS
  These genes may provide an important clue towards our understanding the mechanism of occurrence and the development of periodontal disease.
authors.affiliation: Department of Periodontics, School of Stomatology, China Medical University, Shenyang, China.
authors.forename: Li
authors.initials: L
authors.lastname: Lin
authors.forename: Chen
authors.initials: C
authors.lastname: Li
authors.forename: Jingbo
authors.initials: J
authors.lastname: Liu
authors.forename: Dongmei
authors.initials: D
authors.lastname: Zhang
authors.forename: Jian
authors.initials: J
authors.lastname: Zhao
authors.forename: Yurong
authors.initials: Y
authors.lastname: Kou
authors.forename: Ning
authors.initials: N
authors.lastname: Yu
authors.forename: Yaping
authors.initials: Y
authors.lastname: Pan
mesh_terms: D000328:Adult; D000368:Aged; D016301:Alveolar Bone Loss / Q000382:microbiology; D001431:Bacteriological Techniques; D016866:Bacteroidaceae Infections / Q000382:microbiology; D002232:Carbocyanines; D055113:Chronic Periodontitis / Q000382:microbiology*; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics; D005260:Female; D005456:Fluorescent Dyes; D005798:Genes, Bacterial / Q000235:genetics*; D005884:Gingival Hemorrhage / Q000382:microbiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis; D017622:Periodontal Attachment Loss / Q000382:microbiology; D010514:Periodontal Pocket / Q000382:microbiology; D016966:Porphyromonas gingivalis / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D014086:Tooth Mobility / Q000382:microbiology; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D002232:Carbocyanines; D015342:DNA Probes; D004269:DNA, Bacterial; D005456:Fluorescent Dyes; D037521:Virulence Factors; C085321:cyanine dye 5