title: Regenerative capability of dental pulp cells after crown fracture.
abstract: The purpose of this study was to evaluate the characteristics of dental pulp cells for tissue engineering derived from the fractured incisal portion of tooth crowns. Thirty Sprague-Dawley rats were used for histological and immunohistochemical analysis of nestin protein expression and to measure levels of mRNAs encoding osteocalcin, osteopontin, bone sialoprotein (BSP), dentin sialoprotein (DSP), heat shock protein (HSP) 27, vascular endothelial growth factor (VEGF), ATP-binding cassette transporter G2 (ABCG2), nestin, and p57(Kip2) . Odontoblasts at the incisal portion in the control group were oriented in a regular pattern, but those in the experimental group were randomly stratified. Immunohistochemically, only a few odontoblasts were positive for nestin at the incisal portion in the experimental group at 2 days. Some cells in the inner area in the control group were positive for nestin, but nestin-positive cells in the experimental group at the incisal portion were not observed. The mRNA expression for osteogenic or odontogenic markers in the experimental group was higher than in the control group. HSP27 mRNA expression in the experimental group at 2 days was higher than in the control group and in the experimental group at 7 days. mRNA expression of stem cell markers, such as ABCG2 and nestin, in the experimental group tended to decrease compared with the control. In conclusion, this study demonstrates that dental pulp stem cells derived from fractured teeth differentiate to osteogenic or odontogenic cells.
authors.affiliation: Department of Clinical Pathophysiology, Tokyo Dental College, Chiba, Japan.
authors.forename: Hidehito
authors.initials: H
authors.lastname: Shima
authors.forename: Kenichi
authors.initials: K
authors.lastname: Matsuzaka
authors.forename: Eitoyo
authors.initials: E
authors.lastname: Kokubu
authors.forename: Takashi
authors.initials: T
authors.lastname: Inoue
mesh_terms: D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D018528:ATP-Binding Cassette Transporters / Q000032:analysis; D000818:Animals; D050761:Cyclin-Dependent Kinase Inhibitor p57 / Q000032:analysis; D003782:Dental Pulp / Q000473:pathology*; D003789:Dental Pulp Exposure / Q000473:pathology; D003809:Dentin, Secondary / Q000473:pathology; D016326:Extracellular Matrix Proteins / Q000032:analysis; D055551:HSP27 Heat-Shock Proteins / Q000032:analysis; D007150:Immunohistochemistry; D058574:Integrin-Binding Sialoprotein / Q000032:analysis; D008297:Male; D064231:Nestin / Q000032:analysis; D009804:Odontoblasts / Q000473:pathology; D009805:Odontogenesis / Q000502:physiology; D015675:Osteocalcin / Q000032:analysis; D010012:Osteogenesis / Q000502:physiology; D053495:Osteopontin / Q000032:analysis; D010750:Phosphoproteins / Q000032:analysis; D047428:Protein Kinase Inhibitors / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration / Q000502:physiology*; D012795:Sialoglycoproteins / Q000032:analysis; D013234:Stem Cells / Q000473:pathology; D023822:Tissue Engineering / Q000379:methods; D019228:Tooth Crown / Q000293:injuries*; D014082:Tooth Fractures / Q000473:pathology*; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis; D000099311:Dentin Sialophosphoprotein
chemical_list: D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D018528:ATP-Binding Cassette Transporters; D050761:Cyclin-Dependent Kinase Inhibitor p57; D016326:Extracellular Matrix Proteins; D055551:HSP27 Heat-Shock Proteins; D058574:Integrin-Binding Sialoprotein; D064231:Nestin; D015675:Osteocalcin; D053495:Osteopontin; D010750:Phosphoproteins; D047428:Protein Kinase Inhibitors; D012333:RNA, Messenger; D012795:Sialoglycoproteins; D042461:Vascular Endothelial Growth Factor A; D000099311:Dentin Sialophosphoprotein; C489249:Abcg2 protein, rat; C486114:Hspb1 protein, rat; C546873:Ibsp protein, rat; C577486:Nes protein, rat; C506897:Spp1 protein, rat; C467498:vascular endothelial growth factor A, rat