title: Requirement for Pak3 in Rac1-induced organization of actin and myosin during Drosophila larval wound healing.
abstract: Rho-family small GTPases regulate epithelial cell sheet migration by organizing actin and myosin during wound healing. Here, we report that Pak3, but not Pak1, is a downstream target protein for Rac1 in wound closure of the Drosophila larval epidermis. Pak3-deficient larvae failed to close a wound hole and this defect was not rescued by Pak1 expression, indicating differential functions of the two proteins. Pak3 localized to the wound margin, which selectively required Rac1. Pak3-deficient larvae showed severe defects in actin-myosin organization at the wound margin and in submarginal cells, which was reminiscent of the phenotypes of Rac1-deficient larvae. These results suggest that Pak3 specifically mediates Rac1 signaling in organizing actin and myosin during Drosophila epidermal wound healing.
authors.affiliation: Department of Biology, Yonsei University, Seodaemun-gu, Seoul, Republic of Korea.
authors.forename: Seung Hee
authors.initials: SH
authors.lastname: Baek
authors.forename: Hae Weon
authors.initials: HW
authors.lastname: Cho
authors.forename: Young-Chang
authors.initials: YC
authors.lastname: Kwon
authors.forename: Ji Hyun
authors.initials: JH
authors.lastname: Lee
authors.forename: Moon Jong
authors.initials: MJ
authors.lastname: Kim
authors.forename: Hyangkyu
authors.initials: H
authors.lastname: Lee
authors.forename: Kwang-Min
authors.initials: KM
authors.lastname: Choe
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000502:physiology*; D004817:Epidermis / Q000473:pathology / Q000502:physiology; D007814:Larva / Q000033:anatomy & histology / Q000502:physiology*; D009218:Myosins / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014945:Wound Healing / Q000502:physiology; D054462:p21-Activated Kinases / Q000235:genetics / Q000378:metabolism*; D020830:rac1 GTP-Binding Protein / Q000235:genetics / Q000378:metabolism*
chemical_list: D000199:Actins; D029721:Drosophila Proteins; D011993:Recombinant Fusion Proteins; D054462:p21-Activated Kinases; D009218:Myosins; D020830:rac1 GTP-Binding Protein