title: Using virally expressed melanoma cDNA libraries to identify tumor-associated antigens that cure melanoma.
abstract: Multiple intravenous injections of a cDNA library, derived from human melanoma cell lines and expressed using the highly immunogenic vector vesicular stomatitis virus (VSV), cured mice with established melanoma tumors. Successful tumor eradication was associated with the ability of mouse lymphoid cells to mount a tumor-specific CD4(+) interleukin (IL)-17 recall response in vitro. We used this characteristic IL-17 response to screen the VSV-cDNA library and identified three different VSV-cDNA virus clones that, when used in combination but not alone, achieved the same efficacy against tumors as the complete parental virus library. VSV-expressed cDNA libraries can therefore be used to identify tumor rejection antigens that can cooperate to induce anti-tumor responses. This technology should be applicable to antigen discovery for other cancers, as well as for other diseases in which immune reactivity against more than one target antigen contributes to disease pathology.
authors.affiliation: Department of Molecular Medicine, Mayo Clinic, Rochester, Minnesota, USA.
authors.forename: Jose
authors.initials: J
authors.lastname: Pulido
authors.forename: Timothy
authors.initials: T
authors.lastname: Kottke
authors.forename: Jill
authors.initials: J
authors.lastname: Thompson
authors.forename: Feorillo
authors.initials: F
authors.lastname: Galivo
authors.forename: Phonphimon
authors.initials: P
authors.lastname: Wongthida
authors.forename: Rosa Maria
authors.initials: RM
authors.lastname: Diaz
authors.forename: Diana
authors.initials: D
authors.lastname: Rommelfanger
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Ilett
authors.forename: Larry
authors.initials: L
authors.lastname: Pease
authors.forename: Hardev
authors.initials: H
authors.lastname: Pandha
authors.forename: Kevin
authors.initials: K
authors.lastname: Harrington
authors.forename: Peter
authors.initials: P
authors.lastname: Selby
authors.forename: Alan
authors.initials: A
authors.lastname: Melcher
authors.forename: Richard
authors.initials: R
authors.lastname: Vile
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000276:immunology* / Q000302:isolation & purification*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D045744:Cell Line, Tumor; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D015723:Gene Library; D005822:Genetic Vectors; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006801:Humans; D020381:Interleukin-17 / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology; D008545:Melanoma / Q000235:genetics / Q000276:immunology* / Q000628:therapy*; D008546:Melanoma, Experimental; D051379:Mice; D009374:Neoplasms, Experimental / Q000276:immunology / Q000628:therapy*; D018116:Vesiculovirus
chemical_list: D000951:Antigens, Neoplasm; D018984:Epitopes, T-Lymphocyte; D018840:HSP70 Heat-Shock Proteins; D020381:Interleukin-17