title: Vesiculation from Pseudomonas aeruginosa under SOS.
abstract: Bacterial infections can be aggravated by antibiotic treatment that induces SOS response and vesiculation. This leads to a hypothesis concerning association of SOS with vesiculation. To test it, we conducted multiple analyses of outer membrane vesicles (OMVs) produced from the Pseudomonas aeruginosa wild type in which SOS is induced by ciprofloxacin and from the LexA noncleavable (lexAN) strain in which SOS is repressed. The levels of OMV proteins, lipids, and cytotoxicity increased for both the treated strains, demonstrating vesiculation stimulation by the antibiotic treatment. However, the further increase was suppressed in the lexAN strains, suggesting the SOS involvement. Obviously, the stimulated vesiculation is attributed by both SOS-related and unrelated factors. OMV subproteomic analysis was performed to examine these factors, which reflected the OMV-mediated cytotoxicity and the physiology of the vesiculating cells under treatment and SOS. Thus, SOS plays a role in the vesiculation stimulation that contributes to cytotoxicity.
authors.affiliation: Department of Biology, The University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA.
authors.forename: Reshma
authors.initials: R
authors.lastname: Maredia
authors.forename: Navya
authors.initials: N
authors.lastname: Devineni
authors.forename: Peter
authors.initials: P
authors.lastname: Lentz
authors.forename: Shatha F
authors.initials: SF
authors.lastname: Dallo
authors.forename: Jiehjuen
authors.initials: J
authors.lastname: Yu
authors.forename: Neal
authors.initials: N
authors.lastname: Guentzel
authors.forename: James
authors.initials: J
authors.lastname: Chambers
authors.forename: Bernard
authors.initials: B
authors.lastname: Arulanandam
authors.forename: William E
authors.initials: WE
authors.lastname: Haskins
authors.forename: Tao
authors.initials: T
authors.lastname: Weitao
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002939:Ciprofloxacin / Q000494:pharmacology; D008264:Macrophages / Q000382:microbiology; D051379:Mice; D046529:Microscopy, Electron, Transmission; D040901:Proteomics; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics* / Q000472:pathogenicity; D013014:SOS Response, Genetics / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D002939:Ciprofloxacin