title: Cigarette smoke dissociates inflammation and lung remodeling in OVA-sensitized and challenged mice.
abstract: We evaluated the effects of cigarette smoke (CS) on lung inflammation and remodeling in a model of ovalbumin (OVA)-sensitized and OVA-challenged mice. Male BALB/c mice were divided into 4 groups: non-sensitized and air-exposed (control); non-sensitized and exposed to cigarette smoke (CS), sensitized and air-exposed (OVA) (50 μg+OVA 1% 3 times/week for 3 weeks) and sensitized and cigarette smoke exposed mice (OVA+CS). IgE levels were not affected by CS exposure. The increases in total bronchoalveolar fluid cells in the OVA group were attenuated by co-exposure to CS, as were the changes in IL-4, IL-5, and eotaxin levels as well as tissue elastance (p<0.05). In contrast, only the OVA+CS group showed a significant increase in the protein expression of IFN-γ, VEGF, GM-CSF and collagen fiber content (p<0.05). In our study, exposure to cigarette smoke in OVA-challenged mice resulted in an attenuation of pulmonary inflammation but led to an increase in pulmonary remodeling and resulted in the dissociation of airway inflammation from lung remodeling.
authors.affiliation: Department of Medicine (LIM-20), School of Medicine, University of Sao Paulo, Sao Paulo, Brazil. dehizume@gmail.com
authors.forename: Deborah C
authors.initials: DC
authors.lastname: Hizume
authors.forename: Alessandra C
authors.initials: AC
authors.lastname: Toledo
authors.forename: Henrique T
authors.initials: HT
authors.lastname: Moriya
authors.forename: Beatriz M
authors.initials: BM
authors.lastname: Saraiva-Romanholo
authors.forename: Francine M
authors.initials: FM
authors.lastname: Almeida
authors.forename: Fernanda M
authors.initials: FM
authors.lastname: Arantes-Costa
authors.forename: Rodolfo P
authors.initials: RP
authors.lastname: Vieira
authors.forename: Marisa
authors.initials: M
authors.lastname: Dolhnikoff
authors.forename: David Itiro
authors.initials: DI
authors.lastname: Kasahara
authors.forename: Milton A
authors.initials: MA
authors.lastname: Martins
mesh_terms: D056151:Airway Remodeling; D000818:Animals; D001249:Asthma / Q000276:immunology / Q000473:pathology*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D003094:Collagen / Q000096:biosynthesis; D016207:Cytokines / Q000032:analysis / Q000378:metabolism; D004195:Disease Models, Animal; D004781:Environmental Exposure; D007073:Immunoglobulin E / Q000097:blood; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin; D011014:Pneumonia / Q000276:immunology / Q000473:pathology*; D012906:Smoke; D014026:Nicotiana / Q000009:adverse effects*
chemical_list: D016207:Cytokines; D012906:Smoke; D007073:Immunoglobulin E; D010047:Ovalbumin; D003094:Collagen