title: Protection of cells from nitric oxide-mediated apoptotic death by glutathione C₆₀ derivative.
abstract: The influence of the glutathione C₆₀ derivative on the cytotoxicity of a highly reactive free radical NO (nitric oxide) has been investigated. Consistent with its cytoprotective abilities, the derivative scavenges ROS (reactive oxygen species) and RNS (reactive nitrogen species) both in vitro and under cell-free conditions. Moreover, the glutathione C₆₀ derivative protected PC12 cells from the cytotoxic effect of the NO-releasing compound, SNP (sodium nitroprusside). Addition of glutathione C₆₀ derivative alone did not induce apoptosis and necrosis. The results suggest that the glutathione C₆₀ derivative has the potential to prevent NO-mediated cell death without evident toxicity.
authors.affiliation: School of Chemical Engineering and Technology, State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150001, Peoples Republic of China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Hu
authors.forename: Chunhua
authors.initials: C
authors.lastname: Zhang
authors.forename: Peiyi
authors.initials: P
authors.lastname: Tang
authors.forename: Cuiyun
authors.initials: C
authors.lastname: Li
authors.forename: Yuhuan
authors.initials: Y
authors.lastname: Yao
authors.forename: Shaofan
authors.initials: S
authors.lastname: Sun
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Yudong
authors.initials: Y
authors.lastname: Huang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D016166:Free Radical Scavengers / Q000494:pharmacology; D037741:Fullerenes / Q000494:pharmacology*; D005978:Glutathione / Q000031:analogs & derivatives* / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism*; D009599:Nitroprusside / Q000633:toxicity; D016716:PC12 Cells; D051381:Rats; D026361:Reactive Nitrogen Species / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D016166:Free Radical Scavengers; D037741:Fullerenes; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; C526952:glutathione C(60)-fullerene complex; D009599:Nitroprusside; D009569:Nitric Oxide; D005978:Glutathione