title: Metabolite profiling and quantitative genetics of natural variation for flavonoids in Arabidopsis.
abstract: Little is known about the range and the genetic bases of naturally occurring variation for flavonoids. Using Arabidopsis thaliana seed as a model, the flavonoid content of 41 accessions and two recombinant inbred line (RIL) sets derived from divergent accessions (Cvi-0×Col-0 and Bay-0×Shahdara) were analysed. These accessions and RILs showed mainly quantitative rather than qualitative changes. To dissect the genetic architecture underlying these differences, a quantitative trait locus (QTL) analysis was performed on the two segregating populations. Twenty-two flavonoid QTLs were detected that accounted for 11-64% of the observed trait variations, only one QTL being common to both RIL sets. Sixteen of these QTLs were confirmed and coarsely mapped using heterogeneous inbred families (HIFs). Three genes, namely TRANSPARENT TESTA (TT)7, TT15, and MYB12, were proposed to underlie their variations since the corresponding mutants and QTLs displayed similar specific flavonoid changes. Interestingly, most loci did not co-localize with any gene known to be involved in flavonoid metabolism. This latter result shows that novel functions have yet to be characterized and paves the way for their isolation.
authors.affiliation: INRA, UMR1318, Institut Jean-Pierre Bourgin, Versailles, France. jeanmarc.routaboul@ensat.fr
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Routaboul
authors.forename: Christian
authors.initials: C
authors.lastname: Dubos
authors.forename: Gilles
authors.initials: G
authors.lastname: Beck
authors.forename: Catherine
authors.initials: C
authors.lastname: Marquis
authors.forename: Przemyslaw
authors.initials: P
authors.lastname: Bidzinski
authors.forename: Olivier
authors.initials: O
authors.lastname: Loudet
authors.forename: Loïc
authors.initials: L
authors.lastname: Lepiniec
mesh_terms: D017360:Arabidopsis / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D001947:Breeding; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism; D005419:Flavonoids / Q000378:metabolism*; D014644:Genetic Variation; D055442:Metabolome; D010802:Phylogeny; D040641:Quantitative Trait Loci; D012639:Seeds / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D005419:Flavonoids; C504539:MYB12 protein, Arabidopsis; D014157:Transcription Factors; D003577:Cytochrome P-450 Enzyme System; C038888:flavonoid 3'-hydroxylase