title: Identification of oral bacterial DNA in synovial fluid of patients with arthritis with native and failed prosthetic joints.
abstract: OBJECTIVE
  We examined the presence of bacterial DNA in synovial fluids of native or clinically aseptically failed prosthetic joints from patients having periodontal disease and arthritis to determine whether there is bacterial spread from the oral cavity to the joints.
  
  
  METHODS
  A total of 36 subjects were enrolled in the study. Among these, 11 were diagnosed with rheumatoid arthritis (RA) and 25 were diagnosed with osteoarthritis (OA). Eight patients with OA and 1 patient with RA had failed prostheses. Synovial fluid was aspirated from the affected hip or knee joint. Pooled subgingival plaque samples were collected, followed by clinical periodontal examination. Bacterial DNA was extracted from the collected synovial fluid and dental plaque samples were followed by polymerase chain reactions and DNA sequence analysis of the 16S-23S rRNA genes.
  
  
  RESULTS
  Of the 36 patients, bacterial DNA was detected in the synovial fluid samples from 5 patients (13.9%): 2 with RA (1 native and 1 failed prosthetic joints) and 3 with OA (1 native and 2 failed prosthetic joints). Of these 5 patients, 2 were diagnosed with periodontitis and had identical bacterial clones (Fusobacterium nucleatum and Serratia proteamaculans, respectively) detected in both the synovial fluid and the dental plaque samples. Fusobacterium nucleatum was the most prevalent, detected in 4 of the 5 positive samples. No cultures were done and no patients were treated with antibiotics or developed clinical infection.
  
  
  CONCLUSIONS
  The present findings of bacterial DNA in the synovial fluid suggest the possibility of organisms translocating from the periodontal tissue to the synovium. We suggest that patients with arthritis or failed prosthetic joints be examined for the presence of periodontal diseases and be treated accordingly.
authors.affiliation: Department of Periodontics, School of Dental Medicine, Case Western Reserve University, Cleveland, OH 44106-4905, USA.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Témoin
authors.forename: Alia
authors.initials: A
authors.lastname: Chakaki
authors.forename: Ali
authors.initials: A
authors.lastname: Askari
authors.forename: Ahmed
authors.initials: A
authors.lastname: El-Halaby
authors.forename: Steven
authors.initials: S
authors.lastname: Fitzgerald
authors.forename: Randall E
authors.initials: RE
authors.lastname: Marcus
authors.forename: Yiping W
authors.initials: YW
authors.lastname: Han
authors.forename: Nabil F
authors.initials: NF
authors.lastname: Bissada
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001172:Arthritis, Rheumatoid / Q000382:microbiology* / Q000628:therapy; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000032:analysis*; D005260:Female; D006622:Hip Prosthesis / Q000382:microbiology; D006801:Humans; D007720:Knee Prosthesis / Q000382:microbiology; D008297:Male; D008875:Middle Aged; D010003:Osteoarthritis / Q000382:microbiology* / Q000628:therapy; D010518:Periodontitis / Q000382:microbiology* / Q000628:therapy; D016133:Polymerase Chain Reaction; D013582:Synovial Fluid / Q000382:microbiology*; D017211:Treatment Failure
chemical_list: D004269:DNA, Bacterial