title: Thermodynamical methods for the optimization of lipase-catalyzed reactions.
abstract: A basic insight on different thermodynamical strategies reported for the optimization of lipase-catalyzed reactions is presented. The significance of selecting the appropriate reaction media in order to enhance selectivity and operational stability of enzymes is discussed. From this analysis, the importance of developing thermodynamic strategies for controlling both the reaction kinetics and equilibrium is emphasized. A theoretical model (Conductor-like Screening Model for Realistic Solvation) for calculating thermodynamic properties in fluid phases is proposed as a powerful tool for predicting equilibrium and kinetic behavior in biocatalytic processes.
authors.affiliation: Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico. edmundo@ibt.unam.mx
authors.forename: Edmundo
authors.initials: E
authors.lastname: Castillo
authors.forename: Alejandro
authors.initials: A
authors.lastname: Torres-Gavilán
authors.forename: Georgina
authors.initials: G
authors.lastname: Sandoval
authors.forename: Alain
authors.initials: A
authors.lastname: Marty
mesh_terms: D055162:Biocatalysis; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry*; D007700:Kinetics; D008049:Lipase / Q000737:chemistry*; D008956:Models, Chemical; D011789:Quantum Theory; D013237:Stereoisomerism; D013816:Thermodynamics
chemical_list: D004800:Enzymes, Immobilized; D008049:Lipase