title: Regioisomer separation and identification of triacylglycerols containing vaccenic and oleic acids, and α- and γ-linolenic acids, in thermophilic cyanobacteria Mastigocladus laminosus and Tolypothrix sp.
abstract: Reversed phase liquid chromatography-atmospheric pressure chemical ionization mass spectrometry (RP-HPLC/APCI-MS) was used for direct analysis of triacylglycerols (TAGs) from different strains of the cyanobacteria Mastigocladus laminosus, Tolypothrix cf. tenuis and Tolypothrix distorta. This technique enabled us to identify and quantify the specific molecular species of TAGs directly from lipid extracts of the cyanobacteria. The regioisomeric series of TAGs having α-linolenic and γ-linolenic and also oleic and cis-vaccenic acids were separated by RP-HPLC and identified by APCI-MS. M. laminosus produced only a few molecular species of TAGs, including both isomers of octadecenoic (oleic and vaccenic) acid, while T. distorta contained tens of molecular species of TAGs having FAs with up to four double bonds (stearidonic acid and including also its positional isomer, i.e. 3,6,9,12-octadecatetraenoic acid) and both positional isomers (α and γ) of linolenic acids. Individual strains of both cyanobacteria exhibited different contents of polyunsaturated fatty acids (Tolypothrix sp.) and different distribution of positional isomers of monoenoic fatty acids in TAGs (M. laminosus).
authors.affiliation: Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, CZ-142 20 Prague 4, Czech Republic. rezanka@biomed.cas.cz
authors.forename: Tomáš
authors.initials: T
authors.lastname: Řezanka
authors.forename: Jaromír
authors.initials: J
authors.lastname: Lukavský
authors.forename: Lucie
authors.initials: L
authors.lastname: Siristova
authors.forename: Karel
authors.initials: K
authors.lastname: Sigler
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D000458:Cyanobacteria / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D007536:Isomerism; D015394:Molecular Structure; D009829:Oleic Acids / Q000032:analysis*; D014280:Triglycerides / Q000032:analysis*; D017965:gamma-Linolenic Acid / Q000032:analysis*
chemical_list: D009829:Oleic Acids; D014280:Triglycerides; D017965:gamma-Linolenic Acid