title: Crystal structures of the Tudor domains of human PHF20 reveal novel structural variations on the Royal Family of proteins.
abstract: The human PHD finger protein 20 (PHF20) is a putative transcription factor. While little is known about its cognate cellular role, antibodies against PHF20 are present in sera from patients with hepatocellular carcinoma, glioblastoma and childhood medulloblastula. PHF20 comprises two N-terminal Tudor domains, a central C2H2-link zinc finger domain and a C-terminal zinc-binding PHD domain, and is a component of some MLL methyltransferase complexes. Here, we report the crystal structures of the N-terminal Tudor domains of PHF20 and highlight the novel structural features of each domain. We also confirm previous studies suggesting that the second Tudor domain of PHF20 exhibits preference for dimethylated histone substrates.
authors.affiliation: Structural Genomics Consortium, University of Toronto, Toronto, Ontario, Canada.
authors.forename: Melanie A
authors.initials: MA
authors.lastname: Adams-Cioaba
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Li
authors.forename: Wolfram
authors.initials: W
authors.lastname: Tempel
authors.forename: Yahong
authors.initials: Y
authors.lastname: Guo
authors.forename: Chuanbing
authors.initials: C
authors.lastname: Bian
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Li
authors.forename: Robert
authors.initials: R
authors.lastname: Lam
authors.forename: Jinrong
authors.initials: J
authors.lastname: Min
mesh_terms: D000595:Amino Acid Sequence; D000951:Antigens, Neoplasm / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites; D014408:Biomarkers, Tumor / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D002648:Child; D018360:Crystallography, X-Ray; D004268:DNA-Binding Proteins; D006657:Histones / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011485:Protein Binding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D014157:Transcription Factors
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; D006657:Histones; C440091:PHF20 protein, human; D014157:Transcription Factors