title: Transcriptional architecture of the primate neocortex.
abstract: Genome-wide transcriptional profiling was used to characterize the molecular underpinnings of neocortical organization in rhesus macaque, including cortical areal specialization and laminar cell-type diversity. Microarray analysis of individual cortical layers across sensorimotor and association cortices identified robust and specific molecular signatures for individual cortical layers and areas, prominently involving genes associated with specialized neuronal function. Overall, transcriptome-based relationships were related to spatial proximity, being strongest between neighboring cortical areas and between proximal layers. Primary visual cortex (V1) displayed the most distinctive gene expression compared to other cortical regions in rhesus and human, both in the specialized layer 4 as well as other layers. Laminar patterns were more similar between macaque and human compared to mouse, as was the unique V1 profile that was not observed in mouse. These data provide a unique resource detailing neocortical transcription patterns in a nonhuman primate with great similarity in gene expression to human.
authors.affiliation: Allen Institute for Brain Science, Seattle, WA 98103, USA.
authors.forename: Amy
authors.initials: A
authors.lastname: Bernard
authors.forename: Laura S
authors.initials: LS
authors.lastname: Lubbers
authors.forename: Keith Q
authors.initials: KQ
authors.lastname: Tanis
authors.forename: Rui
authors.initials: R
authors.lastname: Luo
authors.forename: Alexei A
authors.initials: AA
authors.lastname: Podtelezhnikov
authors.forename: Eva M
authors.initials: EM
authors.lastname: Finney
authors.forename: Mollie M E
authors.initials: MM
authors.lastname: McWhorter
authors.forename: Kyle
authors.initials: K
authors.lastname: Serikawa
authors.forename: Tracy
authors.initials: T
authors.lastname: Lemon
authors.forename: Rebecca
authors.initials: R
authors.lastname: Morgan
authors.forename: Catherine
authors.initials: C
authors.lastname: Copeland
authors.forename: Kimberly
authors.initials: K
authors.lastname: Smith
authors.forename: Vivian
authors.initials: V
authors.lastname: Cullen
authors.forename: Jeremy
authors.initials: J
authors.lastname: Davis-Turak
authors.forename: Chang-Kyu
authors.initials: CK
authors.lastname: Lee
authors.forename: Susan M
authors.initials: SM
authors.lastname: Sunkin
authors.forename: Andrey P
authors.initials: AP
authors.lastname: Loboda
authors.forename: David M
authors.initials: DM
authors.lastname: Levine
authors.forename: David J
authors.initials: DJ
authors.lastname: Stone
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Hawrylycz
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Roberts
authors.forename: Allan R
authors.initials: AR
authors.lastname: Jones
authors.forename: Daniel H
authors.initials: DH
authors.lastname: Geschwind
authors.forename: Ed S
authors.initials: ES
authors.lastname: Lein
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D005260:Female; D006801:Humans; D008253:Macaca mulatta / Q000033:anatomy & histology*; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D046228:Microarray Analysis; D042282:Microdissection; D019579:Neocortex / Q000166:cytology* / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D009434:Neural Pathways / Q000502:physiology; D009474:Neurons; D025341:Principal Component Analysis; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome / Q000502:physiology*
chemical_list: D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D014157:Transcription Factors