title: Development and characterization of 41 novel EST-SSR markers for Pisum sativum (Leguminosae).
abstract: OBJECTIVE
  Expressed sequence tag (EST)-derived simple sequence repeat (SSR) markers were developed in Pisum sativum for further use in genetic studies and breeding programs.
  
  
  RESULTS
  Forty-one novel EST-SSR primers were developed and characterized for size polymorphism in 32 Pisum sativum individuals from four populations from China. In each population, the number of alleles per locus ranged from one to seven, with observed heterozygosity and expected heterozygosity ranging from 0 to 0.8889 and 0 to 0.8400, respectively. Furthermore, 53.7% of these markers could be transferred to the related species, Vicia faba.
  
  
  CONCLUSIONS
  The developed markers have potential for application in the study of genetic diversity, germplasm appraisal, and marker-assisted breeding in pea and other legume species.
authors.affiliation: Institute of Vegetables, Zhejiang Academy of Agricultural Science, Hangzhou, People's Republic of China.
authors.forename: Sheng-Chun
authors.initials: SC
authors.lastname: Xu
authors.forename: Ya-Ming
authors.initials: YM
authors.lastname: Gong
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Mao
authors.forename: Qi-Zan
authors.initials: QZ
authors.lastname: Hu
authors.forename: Gu-Wen
authors.initials: GW
authors.lastname: Zhang
authors.forename: Wan
authors.initials: W
authors.lastname: Fu
authors.forename: Qiang-Qiang
authors.initials: QQ
authors.lastname: Xian
mesh_terms: D002681:China; D017931:DNA Primers / Q000378:metabolism; D020224:Expressed Sequence Tags; D005819:Genetic Markers; D005843:Geography; D018895:Microsatellite Repeats / Q000235:genetics*; D018532:Pisum sativum / Q000235:genetics*
chemical_list: D017931:DNA Primers; D005819:Genetic Markers