title: Reversible clustering of magnetic nanobiocatalysts for high-performance biocatalysis and easy catalyst recycling.
abstract: Reversible clusters of nanobiocatalysts are developed via non-covalent interaction among enzyme-bound iron oxide magnetic nanoparticles. Dissociation of the clusters by shaking during biotransformation enables high catalytic performance, and re-clustering by stopping shaking after reaction allows for easy magnetic separation. The novel concept is demonstrated with alcohol dehydrogenase RDR for the enantioselective reduction of 7-methoxy-2-tetralone.
authors.affiliation: Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore, 117576, Singapore.
authors.forename: Thao P N
authors.initials: TP
authors.lastname: Ngo
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Wen
authors.initials: W
authors.lastname: Wang
authors.forename: Zhi
authors.initials: Z
authors.lastname: Li
mesh_terms: D000426:Alcohol Dehydrogenase / Q000378:metabolism; D055162:Biocatalysis; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005290:Ferric Compounds / Q000737:chemistry; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D013237:Stereoisomerism; D044024:Tetralones / Q000737:chemistry / Q000378:metabolism
chemical_list: C570728:7-methoxy-2-tetralone; D004800:Enzymes, Immobilized; D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; D044024:Tetralones; C000499:ferric oxide; D000426:Alcohol Dehydrogenase