title: Association of ghrelin Leu72Met polymorphism with type 2 diabetes mellitus in Chinese population.
abstract: BACKGROUND
  Ghrelin, a novel endogenous ligand for the growth hormone secretagogue receptor, is considered to implicate the development of the type 2 diabetes mellitus (T2DM). The Leu72Met (+408C>A) polymorphism of the preproghrelin, has been linked to obesity, insulin resistance and diabetes.
  
  
  OBJECTIVE
  To investigate the distribution of ghrelin gene Leu72Met polymorphism and its association with the type 2 diabetes mellitus in Chinese population.
  
  
  METHODS
  We conducted a case-control study on 877 patients with T2DM and 864 controls, which were genotyped by the polymerase chain reaction (PCR) technique, denaturing high performance liquid chromatography (DHPLC) and DNA sequence analysis. Laboratory analyses were carried out in the hospital laboratory.
  
  
  RESULTS
  No significant difference in the Leu72Met genotype distributions and allele frequency was observed between type 2 diabetes mellitus and controls (both P>0.05). The polymorphism was not associated with T2DM. However, among the T2DM group, the patients carrying Leu72Leu genotype had significantly increased levels of FPG and serum creatinine compared with variant genotypes (Leu72Met and Met72Met) (P<0.05). In the control group, the subjects with variant genotypes had significantly increased levels of FINS, HOMA-IR compared with Leu72Leu genotype (P<0.05).
  
  
  CONCLUSIONS
  The Leu72Met polymorphism of the preproghrelin gene was not associated with T2DM in Chinese population. However, it may have some roles in the etiology of insulin resistance.
authors.affiliation: Department of Endocrinology, People's Hospital of Gansu Province, 204 West DongGang Road, Lanzhou City, Gansu Province, China. liujingwelcome@126.com
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.forename: Li-min
authors.initials: LM
authors.lastname: Tian
authors.forename: Ju-xiang
authors.initials: JX
authors.lastname: Liu
authors.forename: Ya-jun
authors.initials: YJ
authors.lastname: Bing
authors.forename: Ji-ping
authors.initials: JP
authors.lastname: Zhang
authors.forename: Yun-Fang
authors.initials: YF
authors.lastname: Wang
authors.forename: Lu-yan
authors.initials: LY
authors.lastname: Zhang
mesh_terms: D001483:Base Sequence; D016022:Case-Control Studies; D002681:China; D002851:Chromatography, High Pressure Liquid; D017931:DNA Primers; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D054439:Ghrelin / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D007930:Leucine / Q000737:chemistry / Q000235:genetics*; D008715:Methionine / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
chemical_list: D017931:DNA Primers; D054439:Ghrelin; D008715:Methionine; D007930:Leucine