title: Investigation of chronic alcohol consumption in rodents via ultra-high-performance liquid chromatography-mass spectrometry based metabolite profiling.
abstract: Alcohol consumption in man, when seen in its extreme form of alcoholism, is a complex and socially disruptive disorder that can result in significant levels of liver injury. Here the rodent "intragastric feeding model" was used together with UHPLC-TOFMS analysis to determine changes in global metabolite profiles for plasma and urine from alcohol treated rats and mice compared to control animals. Multivariate statistical analysis (using principal components analysis, PCA) revealed robust differences between profiles from control and alcohol-treated animals from both species. A large number of metabolites were seen to differ between control and alcohol-treated animals, for both biofluids.
authors.affiliation: Department of Chemical Engineering, Aristotle University of Thessaloniki, 541 24, Greece. gkikae@auth.gr
authors.forename: Helen G
authors.initials: HG
authors.lastname: Gika
authors.forename: Cheng
authors.initials: C
authors.lastname: Ji
authors.forename: Georgios A
authors.initials: GA
authors.lastname: Theodoridis
authors.forename: Filippos
authors.initials: F
authors.lastname: Michopoulos
authors.forename: Neil
authors.initials: N
authors.lastname: Kaplowitz
authors.forename: Ian D
authors.initials: ID
authors.lastname: Wilson
mesh_terms: D000428:Alcohol Drinking / Q000097:blood / Q000378:metabolism* / Q000652:urine; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D000431:Ethanol / Q000008:administration & dosage / Q000494:pharmacology; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D055442:Metabolome / Q000187:drug effects; D055432:Metabolomics / Q000379:methods; D051379:Mice; D008810:Mice, Inbred C57BL; D025341:Principal Component Analysis; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000431:Ethanol