title: Collagen-based proteinaceous binder-pigment interaction study under UV ageing conditions by MALDI-TOF-MS and principal component analysis.
abstract: This study focuses on acquiring information on the degradation process of proteinaceous binders due to ultra violet (UV) radiation and possible interactions owing to the presence of historical mineral pigments. With this aim, three different paint model samples were prepared according to medieval recipes, using rabbit glue as proteinaceus binders. One of these model samples contained only the binder, and the other two were prepared by mixing each of the pigments (cinnabar or azurite) with the binder (glue tempera model samples). The model samples were studied by applying Principal Component Analysis (PCA) to their mass spectra obtained with Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF-MS). The complementary use of Fourier Transform Infrared Spectroscopy to study conformational changes of secondary structure of the proteinaceous binder is also proposed. Ageing effects on the model samples after up to 3000 h of UV irradiation were periodically analyzed by the proposed approach. PCA on MS data proved capable of identifying significant changes in the model samples, and the results suggested different aging behavior based on the pigment present. This research represents the first attempt to use this approach (PCA on MALDI-TOF-MS data) in the field of Cultural Heritage and demonstrates the potential benefits in the study of proteinaceous artistic materials for purposes of conservation and restoration.
authors.affiliation: Department of Mineralogy and Petrology, University of Granada, Campus Fuentenueva s/n, 18071, Granada, Spain.
authors.forename: Julia
authors.initials: J
authors.lastname: Romero-Pastor
authors.forename: Natalia
authors.initials: N
authors.lastname: Navas
authors.forename: Stepanka
authors.initials: S
authors.lastname: Kuckova
authors.forename: Alejandro
authors.initials: A
authors.lastname: Rodríguez-Navarro
authors.forename: Carolina
authors.initials: C
authors.lastname: Cardell
mesh_terms: D000269:Adhesives / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D003094:Collagen / Q000737:chemistry* / Q000378:metabolism; D004396:Coloring Agents / Q000737:chemistry* / Q000378:metabolism; D003300:Copper / Q000737:chemistry / Q000378:metabolism; D017669:Mercury Compounds / Q000737:chemistry / Q000378:metabolism; D010151:Paintings; D025341:Principal Component Analysis / Q000379:methods*; D011485:Protein Binding; D011817:Rabbits; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000269:Adhesives; D004396:Coloring Agents; D017669:Mercury Compounds; D003300:Copper; D003094:Collagen; C034211:cinnabar