title: Assessment of a variable frame (polygonal) method to estimate corneal endothelial cell counts after corneal transplantation.
abstract: OBJECTIVE
  To assess the agreement of the 'polygonal' variable frame cell count option on a confocal microscope after keratoplasty, with planimetry as the reference method.
  
  
  METHODS
  One hundred clear corneal grafts of 83 patients attending the cornea clinic at Gartnavel General Hospital in Glasgow underwent slit-scanning in vivoconfocal microscopy. Endothelial cell images were assessed with the Nidek Advanced Vision Information System (NAVIS), using the polygonal variable frame and the manual fixed-frame methods. Planimetry was used as the reference. The agreement between methods was assessed by Bland-Altman analysis.
  
  
  RESULTS
  Planimetry provided a mean (± SD) endothelial cell density (ECD) of 1348 ± 726 cells/mm(2), a value that was very similar to that found by the polygonal method (1404 ± 784 cells/mm(2)). The fixed-frame method provided lower cell counts with a mean ECD of 1026 ± 610 cells/mm(2) (P<0.001). When compared with the reference ECD, the polygonal method overestimated the ECD only very slightly with a mean difference of 58 cells/mm(2) (limits of agreement, LoA, of -222 and 339 cells/mm(2)). Manual counting underestimated the ECD with a mean difference of -320 cells/mm(2) (LoA -814 and 173 cell/mm(2)).
  
  
  CONCLUSIONS
  Following keratoplasty, endothelial cell counts with the NAVIS polygonal method are in good agreement with planimetry. The 'polygonal' option is proposed as the method of choice for clinical applications with this confocal microscope and a good compromise between reliability and ease of use.
authors.affiliation: Tennent Institute of Ophthalmology, Gartnavel General Hospital, Glasgow, UK. sven.jonuscheit@gcu.ac.uk
authors.forename: S
authors.initials: S
authors.lastname: Jonuscheit
authors.forename: M J
authors.initials: MJ
authors.lastname: Doughty
authors.forename: K
authors.initials: K
authors.lastname: Ramaesh
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002452:Cell Count / Q000379:methods; D048430:Cell Shape; D003316:Corneal Diseases / Q000601:surgery; D003941:Diagnostic Techniques, Ophthalmological; D004728:Endothelium, Corneal / Q000473:pathology*; D006801:Humans; D007429:Intraocular Pressure / Q000502:physiology; D015948:Keratoplasty, Penetrating; D018613:Microscopy, Confocal; D008875:Middle Aged; D015203:Reproducibility of Results; D014792:Visual Acuity / Q000502:physiology; D055815:Young Adult