title: Optimization of a method for preparation and repopulation of the tracheal matrix for allogenic transplantation.
abstract: A method for decellularization of the trachea for subsequent repopulation with allogenic MSC was optimized. Tracheas from C57BL/6 mice were devitalized and repopulated with MSC from BALB/c mice. The tracheal matrix with devitalized mucosa and intact cartilaginous structure, fit for repopulation with allogenic MSC, was obtained by chemical treatment with NaClO4. This approach seemed to be promising for transplantation of allogenic trachea.
authors.affiliation: N. N. Blokhin Russian Oncological Research Center, Russian Academy of Medical Sciences, Moscow, Russia. kisele@inbox.ru
authors.forename: M V
authors.initials: MV
authors.lastname: Kiselevsky
authors.forename: N Yu
authors.initials: NY
authors.lastname: Anisimova
authors.forename: O V
authors.initials: OV
authors.lastname: Lebedinskaya
authors.forename: B E
authors.initials: BE
authors.lastname: Polotskii
authors.forename: M I
authors.initials: MI
authors.lastname: Davydov
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D004847:Epithelial Cells / Q000166:cytology; D005615:Freezing; D006801:Humans; D008285:Major Histocompatibility Complex; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009092:Mucous Membrane / Q000187:drug effects; D010472:Perchlorates / Q000633:toxicity; D017670:Sodium Compounds / Q000633:toxicity; D023822:Tissue Engineering / Q000379:methods*; D014132:Trachea / Q000166:cytology / Q000637:transplantation*; D014184:Transplantation, Homologous
chemical_list: D015415:Biomarkers; D010472:Perchlorates; D017670:Sodium Compounds; C031068:sodium perchlorate