title: Efficient gene transfer into neonatal mouse brain using electroporation.
abstract: In vivo electroporation works as an effective method to transfer exogenous genes into postnatal rodent forebrain. Nevertheless, two deficiencies were found in the reported methods. First, surgical operation brings unnecessary trauma to newborn pups. Second, the procedure was complicated and the transfection efficiency was relatively low. Here we improved the previous electroporation method and make it more simple and efficient. The pulse voltage was decreased to 90 v. DNA injection into one pup's forebrain could be completed within 30 s without any surgical operation. More than 94% of injected neonates survived. Almost 100% of the survivors expressed the introduced gene and the expression persists as long as 20 days after injection. Thus, this method offers a powerful new way for gene function study in postnatal neurogenesis and neural development.
authors.affiliation: Department of Cognitive science, Beijing Institute of Basic Medical Sciences, Beijing, 100850, People's Republic of China.
authors.forename: Xue-Feng
authors.initials: XF
authors.lastname: Ding
authors.forename: Yong-Qi
authors.initials: YQ
authors.lastname: Zhao
authors.forename: Zeng-Yao
authors.initials: ZY
authors.lastname: Hu
authors.forename: Kai
authors.initials: K
authors.lastname: Lin
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.forename: Shu-Hong
authors.initials: SH
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Wu
authors.forename: Li-Ying
authors.initials: LY
authors.lastname: Wu
authors.forename: Tong
authors.initials: T
authors.lastname: Zhao
authors.forename: Xin
authors.initials: X
authors.lastname: Huang
authors.forename: Ying
authors.initials: Y
authors.lastname: Wu
authors.forename: Ling-Ling
authors.initials: LL
authors.lastname: Zhu
authors.forename: Wen-Hong
authors.initials: WH
authors.lastname: Fan
authors.forename: Ming
authors.initials: M
authors.lastname: Fan
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000378:metabolism*; D018274:Electroporation; D018014:Gene Transfer Techniques; D051379:Mice; D008813:Mice, Inbred ICR