title: Phospholipase A2 purification and characterization: a case study.
abstract: We compared here the purification procedures, the pH, the calcium, the bile salts, and the temperature dependencies as well as the catalytic activities on phosphatidylcholine (PC) and phosphatidylethanolamine (PE) of two purified secreted PLA2 from chicken pancreatic (ChPLA2-IB) and chicken intestinal (ChPLA2-IIA) origins. Interestingly, ChPLA2-IB hydrolyzes efficiently both purified PC and PE, whereas ChPLA2-IIA hydrolyzes only PE and not PC, even after a long incubation period. These analytical results clearly indicate that the catalytic activity of ChPLA2-IIA, measured with the pH-stat and using egg yolk as substrate, is mainly due to the hydrolysis of the PE fraction present in egg yolk.
authors.affiliation: Laboratoire de Biochimie et de Génie Enzymatique des Lipases, Ecole Nationale d'Ingénieurs de Sfax, Sfax, Tunisia.
authors.forename: Aida
authors.initials: A
authors.lastname: Karray
authors.forename: Youssef
authors.initials: Y
authors.lastname: Gargouri
authors.forename: Robert
authors.initials: R
authors.lastname: Verger
authors.forename: Sofiane
authors.initials: S
authors.lastname: Bezzine
mesh_terms: D000818:Animals; D001647:Bile Acids and Salts / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002645:Chickens; D004530:Egg Yolk / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007422:Intestines / Q000201:enzymology; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D010179:Pancreas / Q000201:enzymology; D010713:Phosphatidylcholines / Q000378:metabolism*; D010714:Phosphatidylethanolamines / Q000378:metabolism*; D054497:Phospholipases A2, Secretory / Q000302:isolation & purification* / Q000378:metabolism; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D001647:Bile Acids and Salts; D007527:Isoenzymes; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; C483858:phosphatidylethanolamine; D054497:Phospholipases A2, Secretory; D002118:Calcium