title: Therapeutic effect of intravenous bone marrow-derived mesenchymal stem cell transplantation on early-stage LPS-induced acute lung injury in mice.
abstract: OBJECTIVE
  To investigate the effect of intravenous bone marrow-derived mesenchymal stem cell (MSC) transplantation for early intervention of lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice.
  
  
  METHODS
  Thirty-six mice were randomized into control group, PBS-treated ALI group, and MSC-treated ALI group. In the latter two groups, mouse models of ALI were established by intranasal instillation of LPS, and 1 h later, the 4th passage of MSCs isolated from the bone marrow of mice or PBS were administered via the tail vein. The histological findings, lung wet/dry (W/D) weight ratio, neutrophil count and protein and cytokine contents in the bronchoalveolar lavage fluid (BALF), and myeloperoxidase (MPO) level in the lung tissue were analyzed at 24 h after MSC administration. Engraftment of MSCs in the recipient lung was determined by fluorescent PKH26 staining and flow cytometry.
  
  
  RESULTS
  Compared with the control group, PBS-treated ALI group showed significantly higher protein levels, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and neutrophil count in the BALF and MPO content in the lung tissue, with also severe damage of lung histology. MSCs administration significantly reduced the lung W/D weight ratio, the levels of protein, TNF-α, IL-6 and neutrophil count in the BALF and MPO content in the lung tissue, and obviously lessened the lung injury 24 h after the transplantation. MSC administration also significantly increased the level of IL-10 in the BALF.
  
  
  CONCLUSIONS
  Intravenous MSC transplantation can effectively improve the lung histology, attenuate the inflammatory response, reduce pulmonary edema in the early stage of LPS-induced ALI in mice, and such effects are independent of MSC engraftment in the lungs.
authors.affiliation: Departement of Clinical Laboratory, Second Affiliated Hospital of Kunming Medical University, Kunming 650101, China. taiwenlinlin@sohu.com
authors.forename: Wen-Lin
authors.initials: WL
authors.lastname: Tai
authors.forename: Zhao-Xing
authors.initials: ZX
authors.lastname: Dong
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Zhang
authors.forename: Dian-Hua
authors.initials: DH
authors.lastname: Wang
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000628:therapy*; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D016207:Cytokines / Q000378:metabolism; D005260:Female; D008070:Lipopolysaccharides; D008168:Lung / Q000378:metabolism; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D051379:Mice; D009195:Peroxidase / Q000378:metabolism
chemical_list: D016207:Cytokines; D008070:Lipopolysaccharides; D009195:Peroxidase