title: Towards multivalent CD1d ligands: synthesis and biological activity of homodimeric α-galactosyl ceramide analogues.
abstract: A library of dimeric CD1d ligands, containing two α-galactosyl ceramide (α-GalCer) units linked by spacers of varying lengths has been synthesised. The key dimerisation reactions were carried out via copper-catalysed click reactions between a 6"-azido-6"-deoxy-α-galactosyl ceramide derivative and various diynes. Each α-GalCer dimer was tested for its ability to stimulate iNKT cells.
authors.affiliation: School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Jervis
authors.forename: Marie
authors.initials: M
authors.lastname: Moulis
authors.forename: John-Paul
authors.initials: JP
authors.lastname: Jukes
authors.forename: Hemza
authors.initials: H
authors.lastname: Ghadbane
authors.forename: Liam R
authors.initials: LR
authors.lastname: Cox
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Cerundolo
authors.forename: Gurdyal S
authors.initials: GS
authors.lastname: Besra
mesh_terms: D000818:Animals; D055610:Antigens, CD1d / Q000737:chemistry* / Q000276:immunology; D002236:Carbohydrate Conformation; D002384:Catalysis; D002478:Cells, Cultured; D057930:Click Chemistry; D019281:Dimerization; D005699:Galactosylceramides / Q000138:chemical synthesis* / Q000494:pharmacology; D007275:Injections, Intravenous; D007371:Interferon-gamma / Q000097:blood / Q000276:immunology; D008024:Ligands; D051379:Mice; D008810:Mice, Inbred C57BL; D008958:Models, Molecular; D055611:Natural Killer T-Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D011485:Protein Binding; D054852:Small Molecule Libraries / Q000138:chemical synthesis* / Q000494:pharmacology; D013154:Spleen / Q000166:cytology / Q000187:drug effects* / Q000276:immunology
chemical_list: D055610:Antigens, CD1d; D005699:Galactosylceramides; D008024:Ligands; D054852:Small Molecule Libraries; D007371:Interferon-gamma