title: Outer dynein arm light chain 1 is essential for controlling the ciliary response to cyclic AMP in Paramecium tetraurelia.
abstract: The individual role of the outer dynein arm light chains in the molecular mechanisms of ciliary movements in response to second messengers, such as Ca(2+) and cyclic nucleotides, is unclear. We examined the role of the gene termed the outer dynein arm light chain 1 (LC1) gene of Paramecium tetraurelia (ODAL1), a homologue of the outer dynein arm LC1 gene of Chlamydomonas reinhardtii, in ciliary movements by RNA interference (RNAi) using a feeding method. The ODAL1-silenced (ODAL1-RNAi) cells swam slowly, and their swimming velocity did not increase in response to membrane-hyperpolarizing stimuli. Ciliary movements on the cortical sheets of ODAL1-RNAi cells revealed that the ciliary beat frequency was significantly lower than that of control cells in the presence of ≥ 1 mM Mg(2+)-ATP. In addition, the ciliary orientation of ODAL1-RNAi cells did not change in response to cyclic AMP (cAMP). A 29-kDa protein phosphorylated in a cAMP-dependent manner in the control cells disappeared in the axoneme of ODAL1-RNAi cells. These results indicate that ODAL1 is essential for controlling the ciliary response by cAMP-dependent phosphorylation.
authors.affiliation: Department of Environmental Biology and Chemistry, Graduate School of Science and Engineering, University of Toyama, Toyama, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Kutomi
authors.forename: Manabu
authors.initials: M
authors.lastname: Hori
authors.forename: Masaki
authors.initials: M
authors.lastname: Ishida
authors.forename: Takashi
authors.initials: T
authors.lastname: Tominaga
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kamachi
authors.forename: France
authors.initials: F
authors.lastname: Koll
authors.forename: Jean
authors.initials: J
authors.lastname: Cohen
authors.forename: Norico
authors.initials: N
authors.lastname: Yamada
authors.forename: Munenori
authors.initials: M
authors.lastname: Noguchi
mesh_terms: D000595:Amino Acid Sequence; D054468:Axoneme / Q000235:genetics / Q000378:metabolism; D002122:Calcium Chloride / Q000494:pharmacology; D002923:Cilia / Q000187:drug effects / Q000378:metabolism*; D046508:Culture Techniques; D000242:Cyclic AMP / Q000378:metabolism*; D004398:Dyneins / Q000235:genetics / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D017125:Genes, Protozoan; D008124:Locomotion; D008969:Molecular Sequence Data; D016805:Paramecium tetraurelia / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D010766:Phosphorylation; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D015800:Protozoan Proteins; D012333:RNA, Messenger; D000242:Cyclic AMP; D004398:Dyneins; D002122:Calcium Chloride