title: Behavioral and genetic effects promoted by sleep deprivation in rats submitted to pilocarpine-induced status epilepticus.
abstract: The interaction between sleep deprivation and epilepsy has been well described in electrophysiological studies, but the mechanisms underlying this association remain unclear. The present study evaluated the effects of sleep deprivation on locomotor activity and genetic damage in the brains of rats treated with saline or pilocarpine-induced status epilepticus (SE). After 50 days of pilocarpine or saline treatment, both groups were assigned randomly to total sleep deprivation (TSD) for 6 h, paradoxical sleep deprivation (PSD) for 24 h, or be kept in their home cages. Locomotor activity was assessed with the open field test followed by resection of brain for quantification of genetic damage by the single cell gel electrophoresis (comet) assay. Status epilepticus induced significant hyperactivity in the open field test and caused genetic damage in the brain. Sleep deprivation procedures (TSD and PSD) did not affect locomotor activity in epileptic or healthy rats, but resulted in significant DNA damage in brain cells. Although PSD had this effect in both vehicle and epileptic groups, TSD caused DNA damage only in epileptic rats. In conclusion, our results revealed that, despite a lack of behavioral effects of sleep deprivation, TSD and PSD induced genetic damage in rats submitted to pilocarpine-induced SE.
authors.affiliation: Departamento de Psicobiologia, Universidade Federal de São Paulo (UNIFESP), Brazil.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Matos
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Ribeiro
authors.forename: Tathiana A
authors.initials: TA
authors.lastname: Alvarenga
authors.forename: Camila
authors.initials: C
authors.lastname: Hirotsu
authors.forename: Fulvio A
authors.initials: FA
authors.lastname: Scorza
authors.forename: Luciana
authors.initials: L
authors.lastname: Le Sueur-Maluf
authors.forename: Juliana
authors.initials: J
authors.lastname: Noguti
authors.forename: Esper A
authors.initials: EA
authors.lastname: Cavalheiro
authors.forename: Sergio
authors.initials: S
authors.lastname: Tufik
authors.forename: Monica L
authors.initials: ML
authors.lastname: Andersen
mesh_terms: D000818:Animals; D004249:DNA Damage; D006948:Hyperkinesis / Q000235:genetics / Q000503:physiopathology*; D008297:Male; D009043:Motor Activity / Q000502:physiology*; D010862:Pilocarpine; D051381:Rats; D017208:Rats, Wistar; D012892:Sleep Deprivation / Q000235:genetics / Q000503:physiopathology*; D012895:Sleep, REM / Q000502:physiology*; D013226:Status Epilepticus / Q000139:chemically induced / Q000235:genetics / Q000503:physiopathology*
chemical_list: D010862:Pilocarpine