title: Carboxy terminus of heat shock protein (HSP) 70-interacting protein (CHIP) inhibits HSP70 in the heart.
abstract: Heat shock protein (HSP) 70 plays a critical role in protecting the heart from various stressor-induced cell injuries; the mechanism remains to be further understood. The present study aims to elucidate the effect of a probiotics-derived protein, LGG-derived protein p75 (LGP), in alleviating the ischemia/reperfusion (I/R)-induced heart injury. We treated rats with the I/R with or without preadministration with LGP. The levels of HSP70 and carboxy terminus of HSP70-interacting protein (CHIP) in the heart tissue were assessed by enzyme-linked immunosorbent assay (ELISA) and Western blotting. The effect of CHIP on suppression of HSP70 and the effect of LGP on suppression of CHIP were investigated with an I/R rat model and a cell culture model. The results showed that I/R-induced infarction in the heart could be alleviated by pretreatment with LGP. HSP70 was detected in naïve rat heart tissue extracts. I/R treatment significantly suppressed the level of HSP70 and increased the levels of CHIP in the heart. A complex of CHIP/HSP70 was detected in heart tissue extracts. The addition of recombinant CHIP to culture inhibited HSP70 in heart cells. LGP was bound CHIP in heart cells and prevented the CHIP from binding HSP70. In summary, I/R can suppress HSP70 and increase CHIP in heart cells. CHIP can suppress HSP70 that can be prevented by pretreatment with LGP. The results imply that CHIP may be a potential target in the prevention of I/R-induced heart cell injury.
authors.affiliation: Department of Cardiovascular Surgery, Xijing Hospital, 127 Changle West RD, Xi'an, 710032, China.
authors.forename: Bijun
authors.initials: B
authors.lastname: Zhao
authors.forename: Guocheng
authors.initials: G
authors.lastname: Sun
authors.forename: Guanli
authors.initials: G
authors.lastname: Feng
authors.forename: Weixun
authors.initials: W
authors.lastname: Duan
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Zhu
authors.forename: Shaoyang
authors.initials: S
authors.lastname: Chen
authors.forename: Lichao
authors.initials: L
authors.lastname: Hou
authors.forename: Zhenxiao
authors.initials: Z
authors.lastname: Jin
authors.forename: Dinghua
authors.initials: D
authors.lastname: Yi
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000008:administration & dosage; D002316:Cardiotonic Agents / Q000008:administration & dosage; D002460:Cell Line; D018840:HSP70 Heat-Shock Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000378:metabolism* / Q000517:prevention & control; D032383:Myocytes, Cardiac / Q000201:enzymology; D011485:Protein Binding; D059748:Proteolysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D044767:Ubiquitin-Protein Ligases / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D002316:Cardiotonic Agents; D018840:HSP70 Heat-Shock Proteins; C540442:Stub1 protein, rat; D044767:Ubiquitin-Protein Ligases