title: Perturbation of glycerol metabolism in hepatocytes from n3-PUFA-depleted             rats.
abstract: Second generation n3-PUFA-depleted rats represent a good animal model of metabolic syndrome as they display several features of the disease such as liver steatosis, visceral obesity and insulin resistance. The goal of our study was to investigate the influence of n3-PUFA deficiency on hepatic glycerol metabolism. Aquaglyceroporin 9 (AQP9) allows hepatic glycerol transport and consequently contributes to neoglucogenesis. AQP9 knockout mice display hypertriacyl-glycerolemia, one of the hallmarks of the metabolic syndrome. Our data show reduced AQP9 expression at the protein level in n3-PUFA-depleted rats, without any changes at the mRNA levels. [U-¹⁴C]glycerol uptake was increased in hepatocytes from n3-PUFA-depleted animal cells. The apparent discrepancy between decreased AQP9 protein expression, and increased [U-¹⁴C]glycerol uptake could be explained by an observed increase in glycerol kinase activity.
authors.affiliation: Laboratory of Experimental Surgery L. Deloyers, Université Libre                     de Bruxelles, Brussels, Belgium.
authors.forename: Laurence
authors.initials: L
authors.lastname: Portois
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Laurence
authors.initials: L
authors.lastname: Ladrière
authors.forename: Jason
authors.initials: J
authors.lastname: Perret
authors.forename: Karim
authors.initials: K
authors.lastname: Louchami
authors.forename: Nathalie
authors.initials: N
authors.lastname: Gaspard
authors.forename: Emeline
authors.initials: E
authors.lastname: Hupkens
authors.forename: Nargis
authors.initials: N
authors.lastname: Bolaky
authors.forename: Valérie
authors.initials: V
authors.lastname: Delforge
authors.forename: Renaud
authors.initials: R
authors.lastname: Beauwens
authors.forename: Willy J
authors.initials: WJ
authors.lastname: Malaisse
authors.forename: Abdullah
authors.initials: A
authors.lastname: Sener
authors.forename: Yvon A
authors.initials: YA
authors.lastname: Carpentier
authors.forename: Christine
authors.initials: C
authors.lastname: Delporte
mesh_terms: D000818:Animals; D020346:Aquaporins / Q000235:genetics / Q000378:metabolism; D002250:Carbon Radioisotopes; D005231:Fatty Acids, Unsaturated / Q000172:deficiency*; D005260:Female; D005786:Gene Expression Regulation; D005990:Glycerol / Q000378:metabolism*; D005991:Glycerol Kinase / Q000378:metabolism; D022781:Hepatocytes / Q000378:metabolism*; D042541:Intracellular Space / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D051379:Mice; D010743:Phospholipids / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D013997:Time Factors; D014280:Triglycerides / Q000378:metabolism
chemical_list: C491637:Aqp9 protein, rat; D020346:Aquaporins; D002250:Carbon Radioisotopes; D005231:Fatty Acids, Unsaturated; D010743:Phospholipids; D012333:RNA, Messenger; D014280:Triglycerides; D005991:Glycerol Kinase; D005990:Glycerol