title: Size-exclusion SPR sensor chip: application to detection of aggregation and disaggregation of biological particles.
abstract: We propose a novel surface plasmon resonance (SPR) sensor chip with a microfabricated slit array. The microslit excludes micrometre-size objects larger than its slit size from the SPR sensing area, so that it functions as an in situ filter. We demonstrated the sensing of microparticles of different diameters using the chip, and the results show a successful size-exclusion effect. As a demonstration of the biological application, we performed the detection of aggregation and disaggregation of biological particles using sugar-chain-immobilized gold nanoparticles as a test sample.
authors.affiliation: Department of Intelligent Mechanical Systems Engineering, Kagawa University, Takamatsu 761-0396, Japan. terao@eng.kagawa-u.ac.jp
authors.forename: Kyohei
authors.initials: K
authors.lastname: Terao
authors.forename: Kazunori
authors.initials: K
authors.lastname: Shimizu
authors.forename: Nobumitsu
authors.initials: N
authors.lastname: Miyanishi
authors.forename: Seiko
authors.initials: S
authors.lastname: Shimamoto
authors.forename: Takaaki
authors.initials: T
authors.lastname: Suzuki
authors.forename: Hidekuni
authors.initials: H
authors.lastname: Takao
authors.forename: Fumikazu
authors.initials: F
authors.lastname: Oohira
mesh_terms: D050260:Carbohydrate Metabolism; D006046:Gold / Q000737:chemistry; D010316:Particle Size; D037121:Plant Lectins / Q000737:chemistry / Q000378:metabolism; D020349:Surface Plasmon Resonance / Q000379:methods*; D013499:Surface Properties
chemical_list: D037121:Plant Lectins; C017707:Ricinus communis agglutinin-1; D006046:Gold