title: Culturable endophytes of medicinal plants and the genetic basis for their bioactivity.
abstract: The bioactive compounds of medicinal plants are products of the plant itself or of endophytes living inside the plant. Endophytes isolated from eight different anticancer plants collected in Yunnan, China, were characterized by diverse 16S and 18S rRNA gene phylogenies. A functional gene-based molecular screening strategy was used to target nonribosomal peptide synthetase (NRPS) and type I polyketide synthase (PKS) genes in endophytes. Bioinformatic analysis of these biosynthetic pathways facilitated inference of the potential bioactivity of endophyte natural products, suggesting that the isolated endophytes are capable of producing a plethora of secondary metabolites. All of the endophyte culture broth extracts demonstrated antiproliferative effects in at least one test assay, either cytotoxic, antibacterial or antifungal. From the perspective of natural product discovery, this study confirms the potential for endophytes from medicinal plants to produce anticancer, antibacterial and antifungal compounds. In addition, PKS and NRPS gene screening is a valuable method for screening isolates of biosynthetic potential.
authors.affiliation: Faculty of Pharmacy, University of Sydney, Sydney, NSW, 2006, Australia.
authors.forename: Kristin I
authors.initials: KI
authors.lastname: Miller
authors.forename: Chen
authors.initials: C
authors.lastname: Qing
authors.forename: Daniel Man-Yuen
authors.initials: DM
authors.lastname: Sze
authors.forename: Basil D
authors.initials: BD
authors.lastname: Roufogalis
authors.forename: Brett A
authors.initials: BA
authors.lastname: Neilan
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism; D000935:Antifungal Agents / Q000378:metabolism; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D002681:China; D003470:Culture Media; D060026:Endophytes / Q000201:enzymology / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D005658:Fungi / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D010453:Peptide Synthases / Q000235:genetics*; D010802:Phylogeny; D010946:Plants, Medicinal / Q000382:microbiology*; D048630:Polyketide Synthases / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D003470:Culture Media; D012336:RNA, Ribosomal, 16S; D012337:RNA, Ribosomal, 18S; D048630:Polyketide Synthases; D010453:Peptide Synthases; C104385:non-ribosomal peptide synthase