title: Inhibitory effect of andrographolide in 3T3-L1 adipocytes differentiation through the PPARγ pathway.
abstract: Andrographolide (AG), an active compound found in Andrographis paniculate Nees, has been shown to exert anti-inflammatory, anticancer and anti-hyperglycemic effects. However, its biological activities against obesity have not been reported. The purpose of this study was to investigate the effect of AG on the differentiation of 3T3-L1 preadipocytes. We found AG significantly inhibited not only on adipocyte differentiation induced by standard adipogenic agents and MDI, but also on the adipogenesis-related transcription factor, peroxisome proliferator-activated receptor γ (PPARγ), as well as the expressions of the PPARγ targeted genes, such as CD36, LPL, FAS and other adiocyte markers. Taken together, our data showed AG inhibited the early stage of adipogenic differentiation, in part via the inhibition of PPARγ-dependent mechanisms.
authors.affiliation: Shanghai Clinical Center for Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Rui-Jin Hospital, Shanghai Jiao-Tong University School of Medicine, Shanghai, China.
authors.forename: Lina
authors.initials: L
authors.lastname: Jin
authors.forename: Wenqiang
authors.initials: W
authors.lastname: Fang
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.forename: Guojun
authors.initials: G
authors.lastname: Shi
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Li
authors.forename: Ying
authors.initials: Y
authors.lastname: Yang
authors.forename: Jian
authors.initials: J
authors.lastname: Yang
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Zhang
authors.forename: Guang
authors.initials: G
authors.lastname: Ning
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism*; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D018955:CD36 Antigens / Q000187:drug effects; D002460:Cell Line; D004224:Diterpenes / Q000494:pharmacology*; D008071:Lipoprotein Lipase / Q000187:drug effects; D051379:Mice; D009765:Obesity / Q000188:drug therapy / Q000378:metabolism; D047495:PPAR gamma / Q000187:drug effects / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D019014:fas Receptor / Q000187:drug effects
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D018955:CD36 Antigens; D004224:Diterpenes; C491899:Fas protein, mouse; D047495:PPAR gamma; D019014:fas Receptor; C030419:andrographolide; D008071:Lipoprotein Lipase