title: A validated chromatographic method for the determination of flavonoids in Copaifera langsdorffii by HPLC.
abstract: Hydroethanolic extracts of C. langsdorffii leaves have therapeutic potential. This work reports a validated chromatographic method for the quantification of polar compounds in the hydroethanolic extract of C. langsdorffii leaves. A reliable HPLC method was developed using two monolithic columns linked in series (100 x 4.6 mm - C18), with nonlinear gradient elution, and UV detection set at 257 nm. A procedure for the extraction of flavonols was also developed, which involved the use of 70% aqueous ethanol and the addition of benzophenone as the internal standard. The developed method led to a good detection response as the values for linearity were between 10.3 and 1000 microg/mL, and those for recovery between 84.2 and 111.1%. The detection limit ranged from 0.02 to 1.70 microg/mL and the quantitation limit from 0.07 to 5.1 microg/mL, with a maximum RSD of 5.24%. Five compounds, rutin, quercetin-3-O-alpha-L-rhamnopyranoside, kaempferol-3-O-alpha-L-rhamnopyranoside, quercetin and kaempferol, were quantified. This method could, therefore, be used for the quality control of hydroethanolic extracts of Copaifera leaves and their cosmetic and pharmaceutical products.
authors.affiliation: Laboratório de Farmacognosia, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Av. Café, s/n, 14040-903, Ribeirão Preto, SP, Brazil. jpsousa@fcfrp.usp.br
authors.forename: João Paulo B
authors.initials: JP
authors.lastname: de Sousa
authors.forename: Ana Paula S
authors.initials: AP
authors.lastname: Brancalion
authors.forename: Milton G
authors.initials: MG
authors.lastname: Júnior
authors.forename: Jairo K
authors.initials: JK
authors.lastname: Bastos
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007887:Fabaceae / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis*
chemical_list: D005419:Flavonoids