title: Weight loss due to a very low calorie diet differentially affects insulin sensitivity and interleukin-6 serum levels in nondiabetic obese human subjects.
abstract: Inflammatory mechanisms are involved in the pathogenesis of type 2 diabetes with interleukin (IL)-6 being particularly important. While long term exercise has been shown to be associated with reduction in IL-6 serum levels in several reports, the discussion on the effect of dietary intervention on IL-6 serum levels is controversial. In the present study, we aimed to investigate the effect of weight loss due to a very low calorie diet (VLCD) on insulin sensitivity and IL-6 serum levels in nondiabetic obese human individuals. 10 patients with obesity were examined during 12 weeks of a VLCD (800 kcal/d). Body composition was measured by impedance analysis. Blood samples were taken before, during, and after the dietary intervention. Leptin, adiponectin, and IL-6 serum levels were measured by ELISA. The body weight decreased significantly from 123.9±6.2-103.5±5.6 kg with a significant reduction in body fat content (43.2±2.3-36.1±3.1%). Leptin levels exhibited a significant decrease from 56.8±5.6-27.9±5.6 ng/ml while adiponectin levels increased significantly from 7.5±0.9-10.6±1.1 μg/ml. Thereby the leptin-to-adiponectin ratio, a novel marker for insulin sensitivity, significantly improved. Mean IL-6 serum concentrations were within the normal range (3.2±0.8 pg/ml) before the study and were not significantly altered by the nutritional therapy. Despite improvement of insulin sensitivity, IL-6 serum levels did not change throughout the study period, suggesting that in nondiabetic obese human subjects IL-6 might have only a minor role in the impairment of insulin sensitivity.
authors.affiliation: Centre of Endocrinology, Diabetes and Preventive Medicine, University of Cologne, Cologne, Germany.
authors.forename: F
authors.initials: F
authors.lastname: Oberhauser
authors.forename: D M
authors.initials: DM
authors.lastname: Schulte
authors.forename: M
authors.initials: M
authors.lastname: Faust
authors.forename: H
authors.initials: H
authors.lastname: Güdelhöfer
authors.forename: M
authors.initials: M
authors.lastname: Hahn
authors.forename: N
authors.initials: N
authors.lastname: Müller
authors.forename: K
authors.initials: K
authors.lastname: Neumann
authors.forename: W
authors.initials: W
authors.lastname: Krone
authors.forename: M
authors.initials: M
authors.lastname: Laudes
mesh_terms: D054392:Adipokines / Q000097:blood; D000328:Adult; D001786:Blood Glucose / Q000378:metabolism; D001823:Body Composition; D001835:Body Weight; D002097:C-Reactive Protein / Q000378:metabolism; D031204:Caloric Restriction; D003920:Diabetes Mellitus / Q000097:blood; D005215:Fasting / Q000097:blood; D005260:Female; D006801:Humans; D007249:Inflammation / Q000097:blood / Q000150:complications / Q000473:pathology; D007333:Insulin Resistance; D015850:Interleukin-6 / Q000097:blood*; D050356:Lipid Metabolism; D008297:Male; D009765:Obesity / Q000097:blood* / Q000150:complications / Q000178:diet therapy* / Q000473:pathology; D015431:Weight Loss
chemical_list: D054392:Adipokines; D001786:Blood Glucose; C508600:IL6 protein, human; D015850:Interleukin-6; D002097:C-Reactive Protein