title: Angelicae Dahuricae Radix Inhibits Dust Mite Extract-Induced Atopic Dermatitis-Like Skin Lesions in NC/Nga Mice.
abstract: We examined whether Angelicae Dahuricae Radix (AR) suppresses the development of atopic dermatitis (AD)-like skin lesions induced by Dermatophagoides farinae in NC/Nga mice. To investigate the effect of AR, we measured the AD severity score, measured plasma levels of IgE and histamine, and performed histological analysis in NC/Nga mice. We also confirmed the anti-inflammatory effects of AR by measuring TARC/CCL17 production from LPS-treated RAW 264.7 cells and mRNA levels of TARC and MDC/CCL22 in TNF-α/IFN-γ-treated HaCaT cells. 10 mg/day of AR extract was applied for 4 weeks to NC/Nga mice. Both the AR extract and 0.1% tacrolimus suppressed the development of AD-like skin lesions and reduced dermatitis scores of the back and ear skin. AR extracts caused an inhibition of histological changes induced by repeated application of D. farinae and a reduction of IgE and histamine levels in plasma (P < 0.05). Furthermore, NO production in LPS-treated RAW 264.7 cells was diminished in a dose-dependent manner, and hTARC production and TARC and MDC mRNA levels in TNF-α/IFN-γ-treated HaCaT cells were diminished by AR. The inhibitory effect of AR on NO, TARC and MDC production may be associated with the suppression of AD-like skin lesions in D. farinae-induced NC/Nga mice.
authors.affiliation: Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine, 483 Expo-ro, Daejeon Yuseong-gu, 305-811, Republic of Korea.
authors.forename: Hoyoung
authors.initials: H
authors.lastname: Lee
authors.forename: Jun Kyoung
authors.initials: JK
authors.lastname: Lee
authors.forename: Hyekyung
authors.initials: H
authors.lastname: Ha
authors.forename: Mee-Young
authors.initials: MY
authors.lastname: Lee
authors.forename: Chang-Seob
authors.initials: CS
authors.lastname: Seo
authors.forename: Hyeun Kyoo
authors.initials: HK
authors.lastname: Shin