title: Crystal structure of agkisacucetin, a Gpib-binding snake C-type lectin that inhibits platelet adhesion and aggregation.
abstract: Agkisacucetin is a snake C-type lectin isolated from the venom of Agkistrodon acutus (A. acutus). It binds specifically to the platelet glycoprotein (GP) Ib and prevents the von Willebrand factor (VWF) accessing it. We determined the crystal structure of agkisacucetin to 1.9Å resolution. The structure of agkisacucetin has an (αβ) fold similar to another GPIb-binding protein, flavocetin-A, but lacks the C-terminal cysteine in the β-subunit, does not form (βα)(4) tetramers, and does not cluster GPIbs, like flavocetin-A.
authors.affiliation: Hefei National Laboratory for Physical Sciences at Microscale, School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026, China.
authors.forename: Yongxiang
authors.initials: Y
authors.lastname: Gao
authors.forename: Honghua
authors.initials: H
authors.lastname: Ge
authors.forename: Hongkai
authors.initials: H
authors.lastname: Chen
authors.forename: Heng
authors.initials: H
authors.lastname: Li
authors.forename: Yiwei
authors.initials: Y
authors.lastname: Liu
authors.forename: Lan
authors.initials: L
authors.lastname: Chen
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Liwen
authors.initials: L
authors.lastname: Niu
authors.forename: Maikun
authors.initials: M
authors.lastname: Teng
mesh_terms: D000595:Amino Acid Sequence; D001798:Blood Proteins / Q000737:chemistry / Q000378:metabolism; D003435:Crotalid Venoms / Q000737:chemistry* / Q000378:metabolism; D018360:Crystallography, X-Ray; D037181:Lectins, C-Type / Q000737:chemistry* / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010975:Platelet Aggregation Inhibitors / Q000737:chemistry / Q000378:metabolism; D011487:Protein Conformation; D021122:Protein Subunits; D016415:Sequence Alignment; D014841:von Willebrand Factor
chemical_list: D001798:Blood Proteins; D003435:Crotalid Venoms; D037181:Lectins, C-Type; D008562:Membrane Glycoproteins; D010975:Platelet Aggregation Inhibitors; D021122:Protein Subunits; C401844:agkisacutacin; C024875:platelet adhesion inhibitor; D014841:von Willebrand Factor; C000592236:agkisacucetin protein, Agkistrodon acutus