title: Analysis of locality of early-stage maturation in confluent state of human retinal pigment epithelial cells.
abstract: Retinal pigment epithelial (RPE) cells were cultured on the laminin-coated and plain surfaces. The measurement of local nucleus density in non-stratified region, which correlated with formation of tight junction, is the indicator of the maturation, and the parameters can be applied to the evaluation of the early-stage maturation of RPE cells in culture.
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Mee-Hae
authors.initials: MH
authors.lastname: Kim
authors.forename: Rie
authors.initials: R
authors.lastname: Sonoi
authors.forename: Kenta
authors.initials: K
authors.lastname: Yamada
authors.forename: Masakazu
authors.initials: M
authors.lastname: Inamori
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kino-oka
mesh_terms: D002468:Cell Physiological Phenomena; D004847:Epithelial Cells / Q000502:physiology; D006801:Humans; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000502:physiology*; D019108:Tight Junctions / Q000502:physiology