title: A high-flux isopore micro-fabricated membrane for effective concentration and recovering of waterborne pathogens.
abstract: A high-flux metallic micro/nano-filtration membrane has been fabricated and validated for isolation of waterborne pathogens from drinking water. Obtained membrane with smooth surface and perfectly ordered pores was achieved by a high yield and cost effective multilevel lithography and electroplating technique. The micro-fabricated membrane was also strengthened with an integrated back-support, which can withstand a high pressure during filtration. The results of microfiltration tests with model particles revealed the superior performance of the micro-fabricated filter than current commercial filters in sample throughput, recovery ratio, and reusability. This study highlighted the potential application of micro-fabricated filer in rapid filtration and recovery of C. parvum oocysts for downstream analysis.
authors.affiliation: School of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore, Singapore.
authors.forename: Majid Ebrahimi
authors.initials: ME
authors.lastname: Warkiani
authors.forename: Chao-Ping
authors.initials: CP
authors.lastname: Lou
authors.forename: Hao-Bing
authors.initials: HB
authors.lastname: Liu
authors.forename: Hai-Qing
authors.initials: HQ
authors.lastname: Gong
mesh_terms: D016785:Cryptosporidium parvum / Q000302:isolation & purification; D005374:Filtration / Q000295:instrumentation*; D007840:Latex; D008567:Membranes, Artificial; D055616:Microtechnology / Q000295:instrumentation*; D033661:Oocysts / Q000382:microbiology; D016062:Porosity; D014871:Water Microbiology; D014873:Water Pollutants / Q000302:isolation & purification*
chemical_list: D007840:Latex; D008567:Membranes, Artificial; D014873:Water Pollutants