title: [Long-term culture and identification of CD133⁺ hematopoietic progenitor cells from human umbilical cord blood].
abstract: OBJECTIVE
  To isolate CD133(+) hematopoietic progenitor cells from human umbilical cord blood and optimize the culture condition for maintaining their stem cell characteristics.
  
  
  METHODS
  CD133(+) hematopoietic progenitor cells were isolated from human umbilical cord blood using magnetic cell sorting system, and the cells were detected by flow cytometry. Four methods were used for culturing cells. After 8 weeks' culture, cytomorphology, flow cytometry, immunocytochemistry and immunofluorescence assay were used to identify the characteristics of the stem cells.
  
  
  RESULTS
  Over 80% of CD133(+) hematopoietic progenitor cells were isolated from human umbilical cord blood using magnetic cell sorting system. The cells were effectively expanded using optimized serum-free medium after 8 weeks of cell culture, whereas the cells in other media differentiated into adherent cells in a poor state.
  
  
  CONCLUSIONS
  The optimized serum-free medium allows effective expansion of CD133(+) hematopoietic progenitor cells that maintain stem cell characteristics after a long-term culture.
authors.affiliation: Department of Pathology and Guangdong Provincial Key Laboratory of Molecular Tumor Pathology, Southern Medical University, Guangzhou 510515, China. chengchengwxj@163.com
authors.forename: Xiao-Jin
authors.initials: XJ
authors.lastname: Wu
authors.forename: Fang
authors.initials: F
authors.lastname: Chen
authors.forename: Yan-Xia
authors.initials: YX
authors.lastname: Lu
authors.forename: Hui
authors.initials: H
authors.lastname: Yang
authors.forename: Fan-Li
authors.initials: FL
authors.lastname: Peng
authors.forename: Li
authors.initials: L
authors.lastname: Yuan
authors.forename: Guo-Bing
authors.initials: GB
authors.lastname: Liu
authors.forename: Xue-Nong
authors.initials: XN
authors.lastname: Li
mesh_terms: D000071916:AC133 Antigen; D015703:Antigens, CD / Q000378:metabolism*; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D046508:Culture Techniques / Q000379:methods*; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D006023:Glycoproteins / Q000378:metabolism*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D010455:Peptides / Q000378:metabolism*
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D016895:Culture Media, Serum-Free; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides