title: Purification and cellular localization of the Entamoeba histolytica transcarboxylase.
abstract: Genome analysis of Entamoeba histolytica predicts the presence of acetyl-CoA carboxylase. Using Western blot, histochemistry, and confocal microscopy, we demonstrated the presence of a biotin-containing protein in the cytoplasm of E. histolytica, with a molecular weight of 136 kDa and biotin-carboxylase activity. This protein probably corresponds to a transcarboxylase that catalyzes the rate-limiting reaction leading to fatty acid elongation.
authors.affiliation: Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luís y Díaz Mirón, CP 11340, Mexico, D.F., Mexico.
authors.forename: E
authors.initials: E
authors.lastname: Barbosa-Cabrera
authors.forename: A
authors.initials: A
authors.lastname: Salas-Casas
authors.forename: S
authors.initials: S
authors.lastname: Rojas-Hernández
authors.forename: A
authors.initials: A
authors.lastname: Jarillo-Luna
authors.forename: E
authors.initials: E
authors.lastname: Abarca-Rojano
authors.forename: M A
authors.initials: MA
authors.lastname: Rodríguez
authors.forename: R
authors.initials: R
authors.lastname: Campos-Rodríguez
mesh_terms: D000818:Animals; D019878:Carboxyl and Carbamoyl Transferases / Q000235:genetics / Q000378:metabolism*; D004748:Entamoeba histolytica / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D018503:Genome, Protozoan; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D015800:Protozoan Proteins; D019878:Carboxyl and Carbamoyl Transferases; C021580:Methylmalonyl-CoA carboxytransferase