title: Molecular cloning and characterization of antimicrobial peptides from skin of the broad-folded frog, Hylarana latouchii.
abstract: Seven cDNA sequences encoding antimicrobial peptide (AMP) precursors were cloned by screening the skin-derived cDNA library of the broad-folded frog, Hylarana latouchii. Seven of the deduced peptides are highly similar to AMPs in five families of brevinin-2 (brevinin-2LTa, brevinin-2LTb, and brevinin-2LTc), esculentin-1 (esculentin-1LTa), esculentin-2 (esculentin-2LTa), palustrin-2 (palustrin-2LTa), and temporin (temporin-LTe). The actual sequences and characteristics of mature AMPs were analyzed by RP-HPLC and LC-MS/MS-based proteomics approaches in combination of four different protein digestion processes and by LTQ XL in combination of gas-phase fractionation (GPF) analysis. Moreover, most of the peptides found in this study hardly display hemolytic activity in vitro, suggesting they are promising antimicrobial drug candidates.
authors.affiliation: Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Sciences, Hebei Normal University, 20 Nanerhuan Dong Road, Shijiazhuang, Hebei 050016, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Yu
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Hu
authors.forename: Haining
authors.initials: H
authors.lastname: Yu
authors.forename: Ran
authors.initials: R
authors.lastname: Ran
authors.forename: Jiangnan
authors.initials: J
authors.lastname: Xia
authors.forename: Duo
authors.initials: D
authors.lastname: Wang
authors.forename: Shujie
authors.initials: S
authors.lastname: Yang
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Yang
authors.forename: Jingze
authors.initials: J
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry* / Q000494:pharmacology*; D001483:Base Sequence; D003001:Cloning, Molecular; D005260:Female; D053878:Hemolytic Agents / Q000032:analysis; D006801:Humans; D007928:Lethal Dose 50; D008297:Male; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000494:pharmacology*; D011898:Ranidae; D012867:Skin / Q000737:chemistry
chemical_list: D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D053878:Hemolytic Agents; D010455:Peptides