title: Simultaneous determination of components released from dental composite resins in human saliva by liquid chromatography/multiple-stage ion trap mass spectrometry.
abstract: Dental composite resins are widely used for fixing teeth; however, the monomers used in dental composite resins have been found to be cytotoxic and genotoxic, namely triethylene glycol dimethacrylate (TEGDMA), urethane dimethacrylate (UDMA), and bisphenol A glycol dimethacrylate (Bis-GMA). In this study, we incubated dental composite resins with human saliva for demonstrating the released monomers and biodegradation products. A simple saliva sample dilution method without purification or derivatization was used for quantification. We found that liquid chromatography coupled with multiple-stage ion trap mass spectrometry (LC-MS(n) ) operated in selected reaction monitoring (SRM) mode was able to separate the three monomers within 10 min. The calibration curves were linear (R² >0.996) over a wide range for each monomer in saliva: TEGDMA, 5-500 ppb; UDMA, 5-100 ppb, and Bis-GMA, 5-700 ppb. Furthermore, several biodegradation products were discovered with data-dependent MS/MS scan techniques. Although TEGMA degradation products have previously been reported, we identified two previously unknown UDMA degradation products. The LC-MS/MS method developed in this study was able to successfully quantify monomers and their principal biodegradation products from dental composite resins in human saliva.
authors.affiliation: Department of Medical Research, China Medical University Hospital, Taichung, Taiwan.
authors.forename: Wei-Yi
authors.initials: WY
authors.lastname: Hsu
authors.forename: Ven-Shing
authors.initials: VS
authors.lastname: Wang
authors.forename: Chien-Chen
authors.initials: CC
authors.lastname: Lai
authors.forename: Fuu-Jen
authors.initials: FJ
authors.lastname: Tsai
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D003188:Composite Resins / Q000737:chemistry*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D011140:Polyurethanes / Q000737:chemistry*; D015203:Reproducibility of Results; D012463:Saliva / Q000737:chemistry*
chemical_list: D000180:Acrylic Resins; C029012:Composite Dental Resin; D003188:Composite Resins; D011140:Polyurethanes