title: Microsatellites in Aureliana fasciculata var. fasciculata (Solanaceae), a shrub that inhabits the Atlantic Rainforest.
abstract: OBJECTIVE
  Microsatellite primers were developed in Aureliana fasciculata var. fasciculata, a shrub that inhabits the Atlantic Rainforest, to investigate the intraspecific genetic structure of the species.
  
  
  RESULTS
  Nine pairs of microsatellite primers were designed that were based on a simple sequence repeat (SSR)-enriched library. Seven loci were amplified successfully. Two populations of A. fasciculata var. fasciculata were genotyped and all loci were polymorphic, with two to five alleles per locus per population detected. Cross-species amplification was achieved in other members of the genus and members of the sister genus Athenaea.
  
  
  CONCLUSIONS
  The development of these microsatellite markers will contribute to future population genetic studies in A. fasciculata var. fasciculata. Cross-amplification among other species in the genus and in the sister genus Athenaea might be a useful tool to establish patterns of genetic variability within the Atlantic Rainforest.
authors.affiliation: Laboratory of Molecular Evolution, Department of Genetics, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
authors.forename: Priscilla M
authors.initials: PM
authors.lastname: Zamberlan
authors.forename: Michele S
authors.initials: MS
authors.lastname: da Silva
authors.forename: Aline M C
authors.initials: AM
authors.lastname: Ramos-Fregonezi
authors.forename: Sandro L
authors.initials: SL
authors.lastname: Bonatto
authors.forename: Loreta B
authors.initials: LB
authors.lastname: Freitas
mesh_terms: D001267:Atlantic Ocean; D017753:Ecosystem; D056426:Genetic Loci / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D019657:Solanaceae / Q000235:genetics*; D014197:Trees