title: An ultrasensitive electrochemical DNA sensor based on the ssDNA-assisted cascade of hybridization reaction.
abstract: We have developed a simple and ultrasensitive E-DNA sensor based on the ssDNA-assisted cascade of a hybridization reaction mechanism to form a long concatamers structure to improve its sensitivity, significantly. The proposed sensor was applied to sequence-specific DNA and ATP detection. Experimental results showed a quantitative measurement with the detection limit as low as 1 aM for specific DNA and 10 fM for ATP.
authors.affiliation: Lab of Biosystem and Microanalysis, East China University of Science & Technology, Shanghai 200237, P.R. China.
authors.forename: Bin-Cheng
authors.initials: BC
authors.lastname: Yin
authors.forename: Yi-Meng
authors.initials: YM
authors.lastname: Guan
authors.forename: Bang-Ce
authors.initials: BC
authors.lastname: Ye
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis; D015374:Biosensing Techniques; D004247:DNA / Q000032:analysis*; D004277:DNA, Single-Stranded / Q000737:chemistry*; D055664:Electrochemical Techniques; D009693:Nucleic Acid Hybridization
chemical_list: D004277:DNA, Single-Stranded; D000255:Adenosine Triphosphate; D004247:DNA