title: New regulators of biofilm development in Candida glabrata.
abstract: Biofilm formation plays an important role in fungal pathogenesis. In this work, we used a genetic screen in order to identify and characterize genes involved in the formation of biofilms by the opportunistic fungal pathogen Candida glabrata. We identified the Cst6p transcription factor as a negative regulator of the EPA6 gene that encodes an adhesin central to C. glabrata biofilm formation. Analysis of single and double mutant strains showed that Cst6p acts in a pathway independent of the Yak1/Sir4 pathway also known to regulate expression of EPA6 and consequently biofilm formation. In contrast, we showed that the chromatin remodelling Swi/Snf complex positively regulates biofilm formation in C. glabrata. RT-qPCR experiments demonstrated that EPA6 expression, and thus biofilm formation, depends on the integrity of the Sir complex. Finally, we showed that Swi/Snf-dependent regulation of biofilm formation is adhesin-specific.
authors.affiliation: Institut Pasteur, Unité des Aspergillus, Département Parasitologie et Mycologie, F-75015 Paris, France. marta.riera@cragenomica.es
authors.forename: Marta
authors.initials: M
authors.lastname: Riera
authors.forename: Estelle
authors.initials: E
authors.lastname: Mogensen
authors.forename: Christophe
authors.initials: C
authors.lastname: d'Enfert
authors.forename: Guilhem
authors.initials: G
authors.lastname: Janbon
mesh_terms: D018441:Biofilms / Q000254:growth & development*; D041221:Candida glabrata / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D005809:Genes, Regulator; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D014157:Transcription Factors