title: Separation and identification of rice prolamins by two-dimensional gel electrophoresis and amino acid sequencing.
abstract: There are difficulties in detecting and separating rice prolamin polypeptides by 2D-PAGE analysis because prolamin polypeptides are insoluble, and the amino acid sequences show high homology among them. In this study, we improved the prolamin extraction method and the 2D-PAGE procedure, and succeeded in separating prolamin polypeptide species by 2D-PAGE and in identifying major prolamin polypeptide sequences.
authors.affiliation: Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo-ku, Kyoto, Japan.
authors.forename: Takanari
authors.initials: T
authors.lastname: Shigemitsu
authors.forename: Yuhi
authors.initials: Y
authors.lastname: Saito
authors.forename: Shigeto
authors.initials: S
authors.lastname: Morita
authors.forename: Shigeru
authors.initials: S
authors.lastname: Satoh
authors.forename: Takehiro
authors.initials: T
authors.lastname: Masumura
mesh_terms: D000595:Amino Acid Sequence; D015180:Electrophoresis, Gel, Two-Dimensional; D012275:Oryza / Q000737:chemistry*; D055315:Prolamins / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification*; D017421:Sequence Analysis
chemical_list: D055315:Prolamins