title: Recombinant monoclonal antibody recognizes a unique epitope on varicella-zoster virus immediate-early 63 protein.
abstract: We previously constructed a recombinant monoclonal antibody (rec-MAb 63P4) that detects immediate-early protein IE63 encoded by varicella-zoster virus (VZV) in the cytoplasm of productively infected cells. Here, we used ORF63 truncation mutants to map the rec-MAb 63P4 binding epitope to amino acids 141 to 150 of VZV IE63, a region not shared with other widely used anti-IE63 antibodies, and found that the recombinant antibody does not bind to the simian IE63 counterpart.
authors.affiliation: Department of Neurology, University of Colorado School of Medicine, Aurora, Colorado, USA.
authors.forename: Niklaus H
authors.initials: NH
authors.lastname: Mueller
authors.forename: Nathan L
authors.initials: NL
authors.lastname: Bos
authors.forename: Scott
authors.initials: S
authors.lastname: Seitz
authors.forename: Mary
authors.initials: M
authors.lastname: Wellish
authors.forename: Ravi
authors.initials: R
authors.lastname: Mahalingam
authors.forename: Don
authors.initials: D
authors.lastname: Gilden
authors.forename: Randall J
authors.initials: RJ
authors.lastname: Cohrs
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D018604:Epitope Mapping; D018985:Epitopes, B-Lymphocyte / Q000276:immunology*; D014645:Herpesvirus 3, Human / Q000235:genetics / Q000276:immunology*; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D018985:Epitopes, B-Lymphocyte; D017874:Immediate-Early Proteins; D014759:Viral Envelope Proteins; C074551:immediate early protein 63, Human herpesvirus 3