title: Molecular characterization of feline infectious peritonitis virus strain DF-2 and studies of the role of ORF3abc in viral cell tropism.
abstract: The full-length genome of the highly lethal feline infectious peritonitis virus (FIPV) strain DF-2 was sequenced and cloned into a bacterial artificial chromosome (BAC) to study the role of ORF3abc in the FIPV-feline enteric coronavirus (FECV) transition. The reverse genetic system allowed the replacement of the truncated ORF3abc of the original FIPV DF-2 genome with the intact ORF3abc of the canine coronavirus (CCoV) reference strain Elmo/02. The in vitro replication kinetics of these two viruses was studied in CrFK and FCWF-4 cell lines, as well as in feline peripheral blood monocytes. Both viruses showed similar replication kinetics in established cell lines. However, the strain with a full-length ORF3 showed markedly lower replication of more than 2 log(10) titers in feline peripheral blood monocytes. Our results suggest that the truncated ORF3abc plays an important role in the efficient macrophage/monocyte tropism of type II FIPV.
authors.affiliation: Department of Virology, Agricultural Office Veterinary Diagnostic Directorate, Budapest, Hungary. balintad@oai.hu
authors.forename: Ádám
authors.initials: Á
authors.lastname: Bálint
authors.forename: Attila
authors.initials: A
authors.lastname: Farsang
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Zádori
authors.forename: Ákos
authors.initials: Á
authors.lastname: Hornyák
authors.forename: László
authors.initials: L
authors.lastname: Dencso
authors.forename: Fernando
authors.initials: F
authors.lastname: Almazán
authors.forename: Luis
authors.initials: L
authors.lastname: Enjuanes
authors.forename: Sándor
authors.initials: S
authors.lastname: Belák
mesh_terms: D000818:Animals; D002415:Cats; D002478:Cells, Cultured; D022202:Chromosomes, Artificial, Bacterial; D003001:Cloning, Molecular; D016765:Coronavirus, Feline / Q000235:genetics* / Q000472:pathogenicity*; D005822:Genetic Vectors; D016679:Genome, Viral; D008969:Molecular Sequence Data; D009000:Monocytes / Q000821:virology; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D056189:Viral Tropism
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins