title: Model of a six immunoglobulin-like domain fragment of filamin A (16-21) built using residual dipolar couplings.
abstract: Filamins are actin-binding proteins that participate in a wide range of cell functions, including cell morphology, locomotion, membrane protein localization, and intracellular signaling. The three filamin isoforms found in humans, filamins A, B, and C, are highly homologous, and their roles are partly complementary. In addition to actin, filamins interact with dozens of other proteins that have roles as membrane receptors and channels, enzymes, signaling intermediates, and transcription factors. Filamins are composed of an N-terminal actin-binding domain and 24 filamin-type immunoglobulin-like domains (FLN) that form tail-to-tail dimers with their C-terminal FLN domain. Many of the filamin interactions including those for glycoprotein Ibα and integrins have been mapped to the region comprising FLN domains 16-21. Traditionally, FLN domains have been viewed as independent folding units, arranged in a linear chain joined with flexible linkers. Recent structural findings have shown that consecutive FLNs form more intricate superstructures. The crystal structure of filamin A domains 19-21 (FLNa19-21) revealed that domains 20 and 21 fold together and that the domain interaction can be autoregulatory. The solution structure of domains 18-19 showed a similar domain interaction, whereas domain pair 16-17 has a completely different domain packing mode. In this study, we characterize the domain organization of the FLNa domain sextet 16-21 using NMR spectroscopy. A structure model of this 60-kDa protein has been built using residual dipolar coupling restraints. RDCs and (15)N relaxation data have been used to characterize interdomain motions.
authors.affiliation: Program in Structural Biology and Biophysics, Institute of Biotechnology, University of Helsinki, Viikinkaari 1, P.O. Box 65, FI-00014 Helsinki, Finland.
authors.forename: Helena
authors.initials: H
authors.lastname: Tossavainen
authors.forename: Outi
authors.initials: O
authors.lastname: Koskela
authors.forename: Pengju
authors.initials: P
authors.lastname: Jiang
authors.forename: Jari
authors.initials: J
authors.lastname: Ylänne
authors.forename: Iain D
authors.initials: ID
authors.lastname: Campbell
authors.forename: Ilkka
authors.initials: I
authors.lastname: Kilpeläinen
authors.forename: Perttu
authors.initials: P
authors.lastname: Permi
mesh_terms: D003285:Contractile Proteins / Q000737:chemistry*; D018360:Crystallography, X-Ray; D064448:Filamins; D006801:Humans; D007128:Immunoglobulin Fragments / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008840:Microfilament Proteins / Q000737:chemistry*; D008958:Models, Molecular; D011487:Protein Conformation
chemical_list: D003285:Contractile Proteins; D064448:Filamins; D007128:Immunoglobulin Fragments; D008840:Microfilament Proteins