title: Identification and pathogenicity analysis of a novel non-tuberculous mycobacterium clinical isolate with nine-antibiotic resistance.
abstract: With mycobacteriosis increasing, the study of non-tuberculous mycobacteria is imperative for clinical therapy and management. Non-tuberculous mycobacteria are naturally resistant to most anti-tuberculosis drugs. Accordingly, it is important to decipher the biology of the novel non-tuberculous mycobacteria through complete genomic analysis of novel pathogenic mycobacteria. We describe Mycobacterium sinense JDM601, a novel, slow-growing mycobacterium of the Mycobacterium terrae complex resistant to nine antibiotics, by clinical presentation, cultural and biochemical characteristics, minimal inhibitory concentrations, and genome-sequencing analysis. JDM601 is closest to Mycobacterium nonchromogenicum according to mycolic acid composition, but closest to Mycobacterium algericum sp. nov according to 16S rDNA. JDM601 is resistant to isoniazid, streptomycin, rifampin, euteropas, protionamide, capromycin, ciprofloxacin, amikacin and levofloxacin but not ethambutol. The clinical information, mycolic acid composition, and virulence genes indicate that JDM601 is an opportunistic pathogen.
authors.affiliation: Department of Medical Microbiology and Parasitology, Institutes of Medical Sciences, Shanghai Jiao-Tong University School of Medicine, Shanghai. Electronic address: shulinzhang@sjtu.edu.cn.
authors.forename: Z-Y
authors.initials: ZY
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Genetic Engineering/Shanghai Public Health Clinical Centre, Fudan University, Shanghai.
authors.forename: Z-Q
authors.initials: ZQ
authors.lastname: Sun
authors.affiliation: Research Centre for Tuberculosis, Henan Chest Hospital, Zhengzhou, Henan, China.
authors.forename: Z-L
authors.initials: ZL
authors.lastname: Wang
authors.affiliation: Department of Medical Microbiology and Parasitology, Institutes of Medical Sciences, Shanghai Jiao-Tong University School of Medicine, Shanghai.
authors.forename: H-R
authors.initials: HR
authors.lastname: Hu
authors.affiliation: Department of Medical Microbiology and Parasitology, Institutes of Medical Sciences, Shanghai Jiao-Tong University School of Medicine, Shanghai.
authors.forename: Z-L
authors.initials: ZL
authors.lastname: Wen
authors.affiliation: State Key Laboratory of Genetic Engineering/Shanghai Public Health Clinical Centre, Fudan University, Shanghai.
authors.forename: Y-Z
authors.initials: YZ
authors.lastname: Song
authors.affiliation: Department of Medical Microbiology and Parasitology, Institutes of Medical Sciences, Shanghai Jiao-Tong University School of Medicine, Shanghai.
authors.forename: J-W
authors.initials: JW
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Genetic Engineering/Shanghai Public Health Clinical Centre, Fudan University, Shanghai.
authors.forename: H-H
authors.initials: HH
authors.lastname: Wang
authors.affiliation: Department of Medical Microbiology and Parasitology, Institutes of Medical Sciences, Shanghai Jiao-Tong University School of Medicine, Shanghai.
authors.forename: X-K
authors.initials: XK
authors.lastname: Guo
authors.affiliation: Department of Medical Microbiology and Parasitology, Institutes of Medical Sciences, Shanghai Jiao-Tong University School of Medicine, Shanghai.
authors.forename: S-L
authors.initials: SL
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000995:Antitubercular Agents / Q000494:pharmacology*; D024901:Drug Resistance, Multiple, Bacterial; D005260:Female; D016680:Genome, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests; D009161:Mycobacterium / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D009164:Mycobacterium Infections / Q000188:drug therapy / Q000382:microbiology*; D010802:Phylogeny; D014774:Virulence / Q000235:genetics*
chemical_list: D000995:Antitubercular Agents