title: Forms of selenium affect its transport, uptake and glutathione peroxidase activity in the Caco-2 cell model.
abstract: The experiment was designed to investigate the effect of selenium (Se) chemical forms (sodium selenite, selenium nanoparticle [nano-Se] and selenomethionine) on the transport, uptake and glutathione peroxidase (GSH-Px) activity in the Caco-2 cell model. The transport and uptake of different forms of Se (0.1 μmol l(-1)) across the Caco-2 cell monolayer were carried out in two directions (apical [AP] to basolateral [BL] and BL to AP) for 2 h, respectively, and the apparent permeability coefficient (P(app)), transport efficiency and uptake efficiency were all calculated. In the present study, the transport and uptake of three forms of Se were time-dependent both in AP to BL and BL to AP directions. By the end of 2 h, the transport efficiencies of selenomethionine and nano-Se were higher than that of sodium selenite (P<0.05). The highest uptake efficiency (P<0.05) was observed in cells treated with nano-Se and significant difference (P<0.05) was also observed between the cells incubated with sodium selenite and selenomethionine. As for the P(app), sodium selenite (P<0.05) had the lowest values compared with that of selenomethionine and nano-Se, in both AP-BL and BL-AP. However, no significant differences were observed in GSH-Px activities. These results indicated that the efficiency of Se in the Caco-2 cells varied with its chemical forms, which might be associated with the differences in Se transport and uptake.
authors.affiliation: Key Laboratory of Food Safety of Zhejiang Province, Food Quality and Safety Department, Zhejiang Gongshang University, 149 Jiaogong Road, Hangzhou 310035 Zhejiang province, China. wangyb@mail.zjgsu.edu.cn
authors.forename: Yanbo
authors.initials: Y
authors.lastname: Wang
authors.forename: Linglin
authors.initials: L
authors.lastname: Fu
mesh_terms: D001692:Biological Transport; D018938:Caco-2 Cells; D004789:Enzyme Activation; D005979:Glutathione Peroxidase / Q000378:metabolism*; D006801:Humans; D008954:Models, Biological; D053758:Nanoparticles / Q000737:chemistry; D010084:Oxidation-Reduction; D012643:Selenium / Q000378:metabolism*; D012645:Selenomethionine / Q000494:pharmacology*; D018038:Sodium Selenite / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D012645:Selenomethionine; D005979:Glutathione Peroxidase; D012643:Selenium; D018038:Sodium Selenite