title: Stable-isotope dilution GC-MS method for ethanol in vapour ethanol and microdialysis systems based on carbonate-catalyzed extractive pentafluorobenzoylation.
abstract: Common ethanol detection methods are not applicable to cell culture media and microdialysates due to interference with medium constituents including amino acids and pH indicators. We present a novel GC-MS method for the accurate and precise analysis of ethanol in cell cultures and microdialysates. The method is based on the carbonate-catalyzed extractive pentafluorobenzoylation of ethanol and deuterium-labelled ethanol serving as the internal standard and on their GC-MS analysis in the electron-capture negative-ion chemical ionization mode. The method was used to optimize experimental conditions in a custom-made ethanol vapour system utilized for studies examining ethanol influences on neuronal cell lines and in microdialysis.
authors.affiliation: Center for Addiction Research, Department of Psychiatry, Socialpsychiatry and Psychotherapy, Hannover Medical School, Hannover, Germany.
authors.forename: Mani
authors.initials: M
authors.lastname: Haschemi Nassab
authors.forename: Anja
authors.initials: A
authors.lastname: Mitschke
authors.forename: Maria-Theresia
authors.initials: MT
authors.lastname: Suchy
authors.forename: Frank-Mathias
authors.initials: FM
authors.lastname: Gutzki
authors.forename: Alexander A
authors.initials: AA
authors.lastname: Zoerner
authors.forename: Mathias
authors.initials: M
authors.lastname: Rhein
authors.forename: Thomas
authors.initials: T
authors.lastname: Hillemacher
authors.forename: Helge
authors.initials: H
authors.lastname: Frieling
authors.forename: Jens
authors.initials: J
authors.lastname: Jordan
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Tsikas
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D001565:Benzoates / Q000737:chemistry*; D002254:Carbonates / Q000737:chemistry*; D002384:Catalysis; D045744:Cell Line, Tumor; D003903:Deuterium / Q000737:chemistry; D000431:Ethanol / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D005740:Gases / Q000032:analysis*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D017551:Microdialysis; D011865:Radioisotope Dilution Technique; D005464:Fluorobenzenes; D005466:Fluorocarbons
chemical_list: D000596:Amino Acids; D001565:Benzoates; D002254:Carbonates; D003903:Deuterium; D000431:Ethanol; D005740:Gases; C014737:pentafluorobenzoyl chloride; D005464:Fluorobenzenes; D005466:Fluorocarbons