title: Simvastatin represses translocation of Pseudomonas aeruginosa across Madin-Darby canine kidney cell monolayers.
abstract: Pseudomonas aeruginosa causes both invasive (bacteremic) and chronic noninvasive infections. An increase in intestinal epithelial permeability is a characteristic of severe sepsis. Alterations in the normal barrier function of the gut mucosa may result in the translocation of microbial cells and products. On the otherhand, it has been demonstrated that statin use is associated with a lower risk of mortality from bloodstream infections. Therefore, we investigated the ability of P. aeruginosa PAO1 to translocate across the Madin-Darby canine kidney (MDCK) cell monolayers in the presence and absence of simvastatin. The bacteria readily translocated across MDCK cell monolayers after 3 h of infection irrespective of the presence or absence of the drug in the medium. However, the bacteria were less able to penetrate the MDCK monolayers in the presence of simvastatin than in its absence. A gentamicin survival assay demonstrated that simvastatin did not affect the bacteria's invasive behavior in the MDCK cells.
authors.affiliation: Department of Molecular Cell Biology and Medicine, Institute of Health Biosciences, the University of Tokushima Graduate School, Tokushima, Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Shibata
authors.forename: Noriko
authors.initials: N
authors.lastname: Nishitani
authors.forename: Sayuri
authors.initials: S
authors.lastname: Yaohara
authors.forename: Naokatu
authors.initials: N
authors.lastname: Arakaki
authors.forename: Tomihiko
authors.initials: T
authors.lastname: Higuti
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Kawazoe
authors.forename: Kazuo
authors.initials: K
authors.lastname: Minakuchi
mesh_terms: D000818:Animals; D000924:Anticholesteremic Agents / Q000633:toxicity; D018988:Bacterial Translocation / Q000187:drug effects*; D002460:Cell Line; D004285:Dogs; D004847:Epithelial Cells / Q000187:drug effects / Q000382:microbiology / Q000473:pathology; D007668:Kidney / Q000166:cytology; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D019821:Simvastatin / Q000633:toxicity*
chemical_list: D000924:Anticholesteremic Agents; D019821:Simvastatin