title: MicroRNA-25 promotes cell migration and invasion in esophageal squamous cell carcinoma.
abstract: MicroRNAs (miRNAs) as a species of small non coding single stranded RNA of about 21-25 nucleotides have important roles in the development of different cancers. In present study, we found that the expression of miR-25 was up-regulated in 60 esophageal squamous cell carcinoma (ESCC) tissues compared with matched adjacent non-cancer tissues. Moreover, we demonstrated that the up-regulation of miR-25 was significantly correlated with the status of lymph node metastasis and TNM (Tumor, Node and Metastasis) stage. Furthermore, over-expression of miR-25 markedly promoted migration and invasion of ESCC cells. On the contrary, down-regulation of miR-25 inhibited the migration and invasion of cells. E-cadherin(CDH1) is a very important tumor metastasis suppressor. We further identified that miR-25 directly targeted CDH1 3'-untranslated region (3'UTR) and repressed the expression of CDH1. These results, for the first time, demonstrate that miR-25 promotes ESCC cell migration and invasion by suppressing CDH1 expression.
authors.affiliation: Department of Thoracic Surgery, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Xu
authors.forename: Zhaoli
authors.initials: Z
authors.lastname: Chen
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Zhao
authors.forename: Jiwen
authors.initials: J
authors.lastname: Wang
authors.forename: Dapeng
authors.initials: D
authors.lastname: Ding
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wang
authors.forename: Fengwei
authors.initials: F
authors.lastname: Tan
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Tan
authors.forename: Fang
authors.initials: F
authors.lastname: Zhou
authors.forename: Jian
authors.initials: J
authors.lastname: Sun
authors.forename: Nan
authors.initials: N
authors.lastname: Sun
authors.forename: Yibo
authors.initials: Y
authors.lastname: Gao
authors.forename: Kang
authors.initials: K
authors.lastname: Shao
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Bin
authors.initials: B
authors.lastname: Qiu
authors.forename: Jie
authors.initials: J
authors.lastname: He
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D015703:Antigens, CD; D015820:Cadherins / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D004938:Esophageal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging
chemical_list: D020413:3' Untranslated Regions; D015703:Antigens, CD; C513384:CDH1 protein, human; D015820:Cadherins; C543351:MIRN25 microRNA, human; D035683:MicroRNAs