title: Monoallelic and biallelic deletions of 13q14.3 in chronic lymphocytic leukemia: FISH vs miRNA RT-qPCR detection.
abstract: Deletion of 13q14.3 (del(13q)) is the most common cytogenetic abnormality in chronic lymphocytic leukemia (CLL) and implies a favorable prognosis. We explored the feasibility of detecting del(13q) by real-time quantitative polymerase chain reaction (PCR) for miR-15a and miR-16-1, whose loci are located in the deleted region. We analyzed 23 cases of B-CLL with monoallelic (10 cases) or biallelic del(13q) (5 cases) and used trisomy 12-positive CLL samples (n = 8) as control samples. As expected, miR-15a was expressed at significantly lower levels in monoallelic del(13qx1) samples compared with trisomy 12 control samples (P = .001). Biallelic del(13q) (del(13qx2)) samples showed further reduction of miR-15a levels compared with monoallelic del(13q) (del(13qx1)) (P = .009). In contrast, miR-16-1 expression levels were generally much lower and variable, with the highest levels detected in del(13qx1). Analyzed retrospectively, miR-15a levels differ among the del(13q) groups. However, only del(13qx2) miR-15a levels are reduced enough to determine the allelic status of an individual sample prospectively by real-time quantitative PCR.
authors.affiliation: Molecular Diagnostics Laboratory, Roswell Park Cancer Institute, Buffalo, NY 14263, USA.
authors.forename: Matthew T
authors.initials: MT
authors.lastname: Smonskey
authors.forename: AnneMarie W
authors.initials: AW
authors.lastname: Block
authors.forename: George
authors.initials: G
authors.lastname: Deeb
authors.forename: Asher A
authors.initials: AA
authors.lastname: Chanan-Khan
authors.forename: Zale P
authors.initials: ZP
authors.lastname: Bernstein
authors.forename: Kena C
authors.initials: KC
authors.lastname: Miller
authors.forename: Paul K
authors.initials: PK
authors.lastname: Wallace
authors.forename: Petr
authors.initials: P
authors.lastname: Starostik
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D002872:Chromosome Deletion; D025063:Chromosome Disorders / Q000235:genetics*; D002882:Chromosomes, Human, Pair 13 / Q000235:genetics; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000235:genetics; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D035683:MicroRNAs