title: Molecular characterization of Giardia parasite isolated from stool samples collected from different hospitals in Taif City (Saudi Arabia).
abstract: Giardia parasite is prevalent endemically in Taif city. Infection is more prevalent   in children under 5 years old and elderly people. The sickness is more intense in immunecompromised   people. The disease is usually diagnosed by stool examination by the microscope,   for the identification of the both trophozoite and cyst stages. Usually the disease is overlooked   during stool analyses due to the minute size of the parasite and due to the scarcity of   infection sometimes. Hence molecular characterization or diagnosis is used as an alternative   method for the diagnosis of infection. Molecular characterization is based on polymerase   chain reaction (PCR). This technique is regarded as a highly sensitive and accurate method   of diagnosis. The prevalence of Giardia duodenalis was 15% in stool samples collected from   different hospital in Taif. By means of RAPD technique, most G. duodenalis isolates were   genetically similar, forming two main groups, with about 60% of similarity one another.
authors.affiliation: Department of Biological Sciences, Faculty of Science, Taif University, Taif, KSA. Youssufgherbawy@yahoo.com
authors.forename: I
authors.initials: I
authors.lastname: Shalaby
authors.forename: Y
authors.initials: Y
authors.lastname: Gherbawy
authors.forename: A
authors.initials: A
authors.lastname: Banaja
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D002648:Child; D019353:Endemic Diseases; D005243:Feces / Q000469:parasitology*; D005260:Female; D005838:Genotype; D016829:Giardia lamblia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D005873:Giardiasis / Q000175:diagnosis / Q000453:epidemiology* / Q000469:parasitology*; D006761:Hospitals; D006801:Humans; D008297:Male; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques; D010274:Parasitology; D016133:Polymerase Chain Reaction; D015995:Prevalence; D019105:Random Amplified Polymorphic DNA Technique; D012529:Saudi Arabia / Q000453:epidemiology