title: Size control of giant unilamellar vesicles prepared from inverted emulsion droplets.
abstract: The production of giant lipid vesicles with controlled size and structure will be an important technology in the design of quantitative biological assays in cell-mimetic microcompartments. For establishing size control of giant vesicles, we investigated the vesicle formation process, in which inverted emulsion droplets are transformed into giant unilamellar vesicles (GUVs) when they pass through an oil/water interface. The relationship between the size of the template emulsion and the converted GUVs was studied using inverted emulsion droplets with a narrow size distribution, which were prepared by microfluidics. We successfully found an appropriate centrifugal acceleration condition to obtain GUVs that had a desired size and narrow-enough size distribution with an improved yield so that emulsion droplets can become the template for GUVs.
authors.affiliation: Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, Yamadaoka 1-5, Suita, Osaka 565-0871, Japan.
authors.forename: Kazuya
authors.initials: K
authors.lastname: Nishimura
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Suzuki
authors.forename: Taro
authors.initials: T
authors.lastname: Toyota
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Yomo
mesh_terms: D004655:Emulsions / Q000138:chemical synthesis / Q000737:chemistry*; D044085:Microfluidics; D009821:Oils / Q000737:chemistry; D010316:Particle Size; D053835:Unilamellar Liposomes / Q000138:chemical synthesis / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D004655:Emulsions; D009821:Oils; D053835:Unilamellar Liposomes; D014867:Water