title: Pharmacokinetics, bioavailability and metabolism of rhaponticin in rat plasma by UHPLC-Q-TOF/MS and UHPLC-DAD-MSn.
abstract: BACKGROUND
  Rhaponticin (Rheum L.) demonstrates a variety of pharmacological activities, including antitumor, antithrombotic and antioxidant effect. However, there is no information describing the pharmacokinetics, bioavailability and metabolism of rhaponticin after intravenous administration.
  
  
  RESULTS
  UHPLC-Q-TOF/MS and UHPLC-multistage tandem MS methods were developed for the pharmacokinetics, bioavailability and metabolism of rhaponticin in rats. The metabolite of rhaponticin, rhapontigenin, a potent inhibitor of cytochrome P450, was confirmed by UHPLC-multistage tandem MS. The plasma profile of rhaponticin and rhapontigenin was determined by UHPLC-Q-TOF/MS. The results showed that rhaponticin was rapidly distributed and eliminated from rat plasma. The absolute oral bioavailability of rhaponticin was calculated to be 0.03%. The plasma concentrations of rhapontigenin rapidly increased and gradually eliminated after intravenous administration.
  
  
  CONCLUSIONS
  The present pharmacokinetics, bioavailability and metabolism studies of rhaponticin will provide helpful information for development of suitable dosage forms and clinical references on rational administration.
authors.affiliation: Department of Traditional Chinese Medicine, The College of Life Sciences, Northwest University, Xi'an, Shaanxi, PR China. zyy@nwu.edu.cn
authors.forename: Ying-Yong
authors.initials: YY
authors.lastname: Zhao
authors.forename: Qi
authors.initials: Q
authors.lastname: Su
authors.forename: Xian-Long
authors.initials: XL
authors.lastname: Cheng
authors.forename: Xiao-Jie
authors.initials: XJ
authors.lastname: Tan
authors.forename: Xu
authors.initials: X
authors.lastname: Bai
authors.forename: Rui-Chao
authors.initials: RC
authors.lastname: Lin
mesh_terms: D000284:Administration, Oral; D000818:Animals; D019540:Area Under Curve; D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D051381:Rats; D013267:Stilbenes / Q000097:blood / Q000737:chemistry / Q000378:metabolism* / Q000493:pharmacokinetics
chemical_list: D013267:Stilbenes; C431126:rhapontigenin; C023538:rhapontin