title: Effect of glacial acetic acid treatment of liquid-based cytology collections on performance of Cervista HPV HR for detection of high-risk human papillomavirus.
abstract: Inadequate cervical cytological analysis can be facilitated by glacial acetic acid (GAA) treatment of primary liquid-based collections to remove mucus, erythrocytes, inflammatory cells, and cellular debris. In the context of a commercial human papillomavirus (HPV) hybridization assay performed on 465 tandem specimens with and without GAA treatment, we show that GAA treatment significantly reduces genomic DNA content (P < 0.0001) and creates an increased potential for indeterminate and false-negative results. In the context of cytological workflow, laboratories should consider providing a specimen aliquot for HPV DNA detection prior to GAA treatment.
authors.affiliation: Wheaton Franciscan Laboratory, Wauwatosa, Wisconsin, USA. Erik.Munson@wfhc.org
authors.forename: Erik
authors.initials: E
authors.lastname: Munson
authors.forename: Brian K
authors.initials: BK
authors.lastname: Du Chateau
authors.forename: Bridget E
authors.initials: BE
authors.lastname: Nelson
authors.forename: Judith
authors.initials: J
authors.lastname: Griep
authors.forename: Jolanta
authors.initials: J
authors.lastname: Czarnecka
authors.forename: Robert D
authors.initials: RD
authors.lastname: Amrhein
authors.forename: Elizabeth R
authors.initials: ER
authors.lastname: Schroeder
mesh_terms: D019342:Acetic Acid / Q000378:metabolism*; D003584:Cytological Techniques / Q000379:methods*; D005188:False Negative Reactions; D005260:Female; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000821:virology; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods*; D014773:Virology / Q000379:methods
chemical_list: D019342:Acetic Acid