title: Specific binding of radiolabeled Cry1Fa insecticidal protein from Bacillus thuringiensis to midgut sites in lepidopteran species.
abstract: Cry1Fa insecticidal protein was successfully radiolabeled with (125)I-Na. Specific binding to brush border membrane vesicles was shown for the lepidopteran species Ostrinia nubilalis, Spodoptera frugiperda, Spodoptera exigua, Helicoverpa armigera, Heliothis virescens, and Plutella xylostella. Homologous competition assays were performed to obtain equilibrium binding parameters (K(d) [dissociation constant] and R(t) [concentration of binding sites]) for these six insect species.
authors.affiliation: Departamento de Genética, Facultad de CC Biológicas, Universidad de Valencia, Burjassot, Valencia, Spain.
authors.forename: Carmen Sara
authors.initials: CS
authors.lastname: Hernández-Rodríguez
authors.forename: Patricia
authors.initials: P
authors.lastname: Hernández-Martínez
authors.forename: Jeroen
authors.initials: J
authors.lastname: Van Rie
authors.forename: Baltasar
authors.initials: B
authors.lastname: Escriche
authors.forename: Juan
authors.initials: J
authors.lastname: Ferré
mesh_terms: D000818:Animals; D001413:Bacillus thuringiensis / Q000378:metabolism*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000378:metabolism*; D001665:Binding Sites; D004064:Digestive System / Q000378:metabolism* / Q000648:ultrastructure; D004731:Endotoxins / Q000378:metabolism*; D006460:Hemolysin Proteins / Q000378:metabolism*; D007457:Iodine Radioisotopes / Q000378:metabolism*; D007915:Lepidoptera / Q000145:classification / Q000378:metabolism*; D008871:Microvilli / Q000378:metabolism; D013045:Species Specificity; D018411:Spodoptera / Q000378:metabolism; D022161:Transport Vesicles / Q000378:metabolism
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; D007457:Iodine Radioisotopes; C030956:insecticidal crystal protein, Bacillus Thuringiensis