title: Tuning multi/pluri-potent stem cell fate by electrospun poly(L-lactic acid)-calcium-deficient hydroxyapatite nanocomposite mats.
abstract: In this study, we investigated whether multipotent (human-bone-marrow-derived mesenchymal stem cells [hBM-MSCs]) and pluripotent stem cells (murine-induced pluripotent stem cells [iPSCs] and murine embryonic stem cells [ESCs]) respond to nanocomposite fibrous mats of poly(L-lactic acid) (PLLA) loaded with 1 or 8 wt % of calcium-deficient nanohydroxyapatite (d-HAp). Remarkably, the dispersion of different amounts of d-HAp to PLLA produced a set of materials (PLLA/d-HAp) with similar architectures and tunable mechanical properties. After 3 weeks of culture in the absence of soluble osteogenic factors, we observed the expression of osteogenic markers, including the deposition of bone matrix proteins, in multi/pluripotent cells only grown on PLLA/d-HAp nanocomposites, whereas the osteogenic differentiation was absent on stem-cell-neat PLLA cultures. Interestingly, this phenomenon was confined only in hBM-MSCs, murine iPSCs, and ESCs grown on direct contact with the PLLA/d-HAp mats. Altogether, these results indicate that the osteogenic differentiation effect of these electrospun PLLA/d-HAp nanocomposites was independent of the stem cell type and highlight the direct interaction of stem cell-polymeric nanocomposite and the mechanical properties acquired by the PLLA/d-HAp nanocomposites as key steps for the differentiation process.
authors.affiliation: Department of Experimental Medicine and Biochemical Sciences, Section of Biochemistry and Molecular Biology, University of Perugia, Via del Giochetto, Perugia, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: D'Angelo
authors.forename: Ilaria
authors.initials: I
authors.lastname: Armentano
authors.forename: Ilaria
authors.initials: I
authors.lastname: Cacciotti
authors.forename: Roberto
authors.initials: R
authors.lastname: Tiribuzi
authors.forename: Mattia
authors.initials: M
authors.lastname: Quattrocelli
authors.forename: Costantino
authors.initials: C
authors.lastname: Del Gaudio
authors.forename: Elena
authors.initials: E
authors.lastname: Fortunati
authors.forename: Enrica
authors.initials: E
authors.lastname: Saino
authors.forename: Auro
authors.initials: A
authors.lastname: Caraffa
authors.forename: Giuliano Giorgio
authors.initials: GG
authors.lastname: Cerulli
authors.forename: Livia
authors.initials: L
authors.lastname: Visai
authors.forename: Josè Maria
authors.initials: JM
authors.lastname: Kenny
authors.forename: Maurilio
authors.initials: M
authors.lastname: Sampaolesi
authors.forename: Alessandra
authors.initials: A
authors.lastname: Bianco
authors.forename: Sabata
authors.initials: S
authors.lastname: Martino
authors.forename: Aldo
authors.initials: A
authors.lastname: Orlacchio
mesh_terms: D000818:Animals; D002118:Calcium / Q000737:chemistry*; D002470:Cell Survival; D017886:Durapatite / Q000737:chemistry; D004563:Electrochemistry; D053595:Embryonic Stem Cells / Q000737:chemistry* / Q000166:cytology; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000737:chemistry* / Q000166:cytology; D051379:Mice; D053761:Nanocomposites / Q000737:chemistry*; D010316:Particle Size; D039904:Pluripotent Stem Cells / Q000737:chemistry* / Q000166:cytology; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*
chemical_list: D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D017886:Durapatite; D002118:Calcium