title: Virus-induced gene silencing of Mlo genes induces powdery mildew resistance in Triticum aestivum.
abstract: Powdery mildew is one of the most important cereal diseases worldwide. Genetic analysis has revealed that mutant alleles of the Mlo gene cause broad-spectrum resistance against this pathogen in barley. In this study, the possibility of inducing broad-spectrum powdery mildew resistance against this pathogen by RNAi of the barley Mlo ortholog in wheat was examined using virus-induced gene silencing (VIGS). A clear correlation was found between resistance and accumulation of Mlo-specific siRNAs, raising the possibility of designing powdery mildew resistance in wheat by RNA silencing using both transgenic and non-transgenic approaches.
authors.affiliation: Agricultural Biotechnology Center, Plant Virology Group, Szent Györgyi A. út 4, Gödöllő 2100, Hungary. varallya@abc.hu
authors.forename: Eva
authors.initials: E
authors.lastname: Várallyay
authors.forename: Gábor
authors.initials: G
authors.lastname: Giczey
authors.forename: József
authors.initials: J
authors.lastname: Burgyán
mesh_terms: D001203:Ascomycota / Q000276:immunology*; D020868:Gene Silencing / Q000502:physiology*; D009154:Mutation; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology* / Q000382:microbiology*; D010940:Plant Proteins / Q000235:genetics*; D014908:Triticum / Q000235:genetics* / Q000276:immunology / Q000382:microbiology
chemical_list: C104922:MLO protein, Hordeum vulgare; D010940:Plant Proteins