title: [Experimental studies on anticoagulant activity of different extract of leech in vitro].
abstract: OBJECTIVE
  To study the anticoagulant activity of different extracts of Leech.
  
  
  METHODS
  Prothrombin time (PT), thrombin time (TT) and activated partial thromboplastin time (APTT) were determined by coagulometer for blood samples collected from abdominal vein of Wistar rat.
  
  
  RESULTS
  PT, TT, APTT were prolonged remarkably by ethyl acetate part of Leech. PT was prolonged remarkably by hexane part and aqueous solution part of Leech. APTT was prolonged remarkably by n-Butylalcohol part of Leech.
  
  
  CONCLUSIONS
  The different extracts of Leech have obvious anticoagulant effects. There are new anticoagulant components different from hirudin in Leech.
authors.affiliation: Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850.
authors.forename: Yanling
authors.initials: Y
authors.lastname: Li
authors.forename: Rongqing
authors.initials: R
authors.lastname: Huang
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Sun
mesh_terms: D000085:Acetates; D000818:Animals; D000925:Anticoagulants / Q000302:isolation & purification / Q000494:pharmacology*; D001777:Blood Coagulation / Q000187:drug effects*; D001780:Blood Coagulation Tests; D007865:Leeches / Q000737:chemistry; D008297:Male; D008419:Materia Medica / Q000302:isolation & purification / Q000494:pharmacology*; D010314:Partial Thromboplastin Time; D011517:Prothrombin Time; D051381:Rats; D017208:Rats, Wistar; D012997:Solvents / Q000737:chemistry; D013918:Thrombin Time
chemical_list: D000085:Acetates; D000925:Anticoagulants; D008419:Materia Medica; D012997:Solvents; C007650:ethyl acetate