title: Optimizing Saccharomyces cerevisiae induction regimes.
abstract: Recombinant membrane protein yields can be optimized in Saccharomyces cerevisiae by adjusting the induction time and temperature and/or by the addition of chemical chaperones. Here we describe a protocol for assessing the importance of these parameters.
authors.affiliation: Division of Molecular Biosciences, Membrane Protein Crystallography Group, Imperial College, London, UK.
authors.forename: David
authors.initials: D
authors.lastname: Drew
authors.forename: Hyun
authors.initials: H
authors.lastname: Kim
mesh_terms: D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D013696:Temperature
chemical_list: D008565:Membrane Proteins; D011994:Recombinant Proteins