title: Prediction of superovulatory response in Japanese Black cattle using ultrasound, plasma anti-Müllerian hormone concentrations and polymorphism in the ionotropic glutamate receptor AMPA1/GRIA1.
abstract: The aim of this study was to improve the reliability of predicting the superovulatory response in Japanese Black cattle. Follicle counts and plasma anti-Müllerian hormone concentrations were analyzed within four days prior to the initiation of superovulation. The single nucleotide polymorphism (guanine or adenine) of the ionotropic glutamate receptor AMPA1 was determined. The plasma anti-Müllerian hormone concentration was positively correlated (P<0.001) with the numbers of all follicles and small (<5 mm) follicles and with the numbers of ova/embryos (P<0.001), fertilized embryos (P<0.001) and transferable embryos (P=0.005). There was no significant difference in follicle counts and superovulatory responses between donor cows bearing guanine/adenine or guanine/guanine alleles of AMPA1. Donor cows with a high plasma anti-Müllerian hormone concentration and homozygous for the guanine-containing allele of AMPA1 were most responsive to superovulation. The results suggest that physiological and genetic markers of superovulation have a synergistic effect on the accuracy of predictions of responsiveness.
authors.affiliation: Animal Biotechnology Group, Animal Research Center, Hokkaido Research Organization, Hokkaido 081-0038, Japan. hirayama-hiroki@hro.or.jp
authors.forename: Hiroki
authors.initials: H
authors.lastname: Hirayama
authors.forename: Soichi
authors.initials: S
authors.lastname: Kageyama
authors.forename: Akira
authors.initials: A
authors.lastname: Naito
authors.forename: Shigeo
authors.initials: S
authors.lastname: Fukuda
authors.forename: Takashi
authors.initials: T
authors.lastname: Fujii
authors.forename: Akira
authors.initials: A
authors.lastname: Minamihashi
mesh_terms: D000225:Adenine / Q000737:chemistry; D000483:Alleles; D000818:Animals; D054304:Anti-Mullerian Hormone / Q000097:blood*; D002417:Cattle; D005260:Female; D005306:Fertilization; D018507:Gene Expression Regulation, Developmental; D005819:Genetic Markers / Q000235:genetics; D006147:Guanine / Q000737:chemistry; D020641:Polymorphism, Single Nucleotide; D018091:Receptors, AMPA / Q000378:metabolism; D015203:Reproducibility of Results; D027724:Reproductive Techniques, Assisted; D013480:Superovulation
chemical_list: D000225:Adenine; D054304:Anti-Mullerian Hormone; D005819:Genetic Markers; D006147:Guanine; D018091:Receptors, AMPA