title: Cytotoxic effect of fenitrothion and lambda-cyhalothrin mixture on lipid peroxidation and antioxidant defense system in rat kidney.
abstract: A mixture of pyrethroids plus organophosphates was assessed for their potential effects on lipid peroxidation, the antioxidant defense system and lactate dehydrogenase (LDH) in rat kidney in vitro. Various insecticide concentrations were incubated with kidney homogenate at 37°C for different incubation times. Treatment with fenitothion (FNT) plus lambda-cyhalothrin (LC) caused a significant induction (P < 0.05) in thiobarbituric acid reactive substances (TBARS), which might be associated to decreased levels of reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST) activities and protein content in rat kidney. However, a significant induction of lactate dehydrogenase (LDH) activity was observed. The effect was concentration and time dependent. It can be concluded that depletion of GSH might indicate that reactive oxygen species (ROS) could be involved in the toxic effects of FNT plus LC which lead to marked perturbations in antioxidant defense system.
authors.affiliation: Institute of Graduate Studies and Research, Alexandria University, Alexandria, Egypt. feldemerdash@yahoo.com
authors.forename: Fatma M
authors.initials: FM
authors.lastname: El-Demerdash
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D004781:Environmental Exposure / Q000009:adverse effects*; D004784:Environmental Monitoring; D005278:Fenitrothion / Q000633:toxicity*; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D006801:Humans; D007306:Insecticides / Q000633:toxicity*; D007668:Kidney / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D009570:Nitriles / Q000633:toxicity*; D018384:Oxidative Stress; D011722:Pyrethrins / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000975:Antioxidants; D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; D017382:Reactive Oxygen Species; D005982:Glutathione Transferase; D005978:Glutathione; C037304:cyhalothrin; D005278:Fenitrothion