title: Destabilization of a bovine B₁₂ trafficking chaperone protein by oxidized form of glutathione.
abstract: The protein, bCblCpro, is a bovine B(12) trafficking chaperone involved in intracellular B(12) metabolism. bCblCpro is highly thermolabile (T(m)=∼42°C) and the reduced form of glutathione, GSH, has been found to stabilize bCblCpro as a positive regulator. In this study, we discovered that the oxidized form of glutathione, GSSG, destabilizes bCblCpro, which is derived from changes in the conformation of the protein upon GSSG binding. The binding affinity for GSSG was determined to be similar with the affinity for GSH. The AC(50)=2.8 ± 0.4 mM of GSSG for destabilization of bCblCpro was consistently similar with the AC(50)=2.1 ± 0.5 mM of GSH for stabilization of the protein. These results suggest that GSSG is a negative regulator of bCblCpro and that the molar ratio of [GSH]/[GSSG] in cells may determine the stability of the B(12) trafficking chaperone.
authors.affiliation: School of Biotechnology, Yeungnam University, Gyeongsan 712-749, Republic of Korea.
authors.forename: Jihyun
authors.initials: J
authors.lastname: Park
authors.forename: Jinju
authors.initials: J
authors.lastname: Jeong
authors.forename: Jihoe
authors.initials: J
authors.lastname: Kim
mesh_terms: D000818:Animals; D001692:Biological Transport; D002417:Cattle; D005978:Glutathione / Q000737:chemistry*; D018832:Molecular Chaperones / Q000737:chemistry* / Q000378:metabolism; D010084:Oxidation-Reduction; D055550:Protein Stability; D014155:Transcobalamins / Q000737:chemistry* / Q000378:metabolism; D014805:Vitamin B 12 / Q000378:metabolism*
chemical_list: D018832:Molecular Chaperones; D014155:Transcobalamins; D005978:Glutathione; D014805:Vitamin B 12