title: Arginase induction represses gall development during clubroot infection in Arabidopsis.
abstract: Arginase induction can play a defensive role through the reduction of arginine availability for phytophageous insects. Arginase activity is also induced during gall growth caused by Plasmodiophora brassicae infection in roots of Arabidopsis thaliana; however, its possible role in this context has been unclear. We report here that the mutation of the arginase-encoding gene ARGAH2 abrogates clubroot-induced arginase activity and results in enhanced gall size in infected roots, suggesting that arginase plays a defensive role. Induction of arginase activity in infected roots was impaired in the jar1 mutant, highlighting a link between the arginase response to clubroot and jasmonate signaling. Clubroot-induced accumulation of the principal amino acids in galls was not affected by the argah2 mutation. Because ARGAH2 was previously reported to control auxin response, we investigated the role of ARGAH2 in callus induction. ARGAH2 was found to be highly induced in auxin/cytokinin-triggered aseptic plant calli, and callus development was enhanced in argah2 in the absence of the pathogen. We hypothesized that arginase contributes to a negative control over clubroot symptoms, by reducing hormone-triggered cellular proliferation.
authors.affiliation: Université Rennes 1, UMR1349 IGEPP, F-35000 Rennes, France. antoine.gravot@univ-rennes1.fr
authors.forename: Antoine
authors.initials: A
authors.lastname: Gravot
authors.forename: Carole
authors.initials: C
authors.lastname: Deleu
authors.forename: Geoffrey
authors.initials: G
authors.lastname: Wagner
authors.forename: Christine
authors.initials: C
authors.lastname: Lariagon
authors.forename: Raphael
authors.initials: R
authors.lastname: Lugan
authors.forename: Christopher
authors.initials: C
authors.lastname: Todd
authors.forename: David
authors.initials: D
authors.lastname: Wendehenne
authors.forename: Régine
authors.initials: R
authors.lastname: Delourme
authors.forename: Alain
authors.initials: A
authors.lastname: Bouchereau
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Manzanares-Dauleux
mesh_terms: D000581:Amidohydrolases / Q000096:biosynthesis* / Q000235:genetics; D000596:Amino Acids / Q000378:metabolism; D017360:Arabidopsis / Q000166:cytology / Q000187:drug effects / Q000201:enzymology* / Q000469:parasitology*; D029681:Arabidopsis Proteins / Q000096:biosynthesis* / Q000235:genetics; D003517:Cyclopentanes / Q000494:pharmacology; D003979:Diazonium Compounds / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D006900:Hydroxylation / Q000187:drug effects; D007532:Isoleucine / Q000031:analogs & derivatives / Q000494:pharmacology; D009154:Mutation / Q000235:genetics; D009928:Organ Specificity / Q000187:drug effects; D054883:Oxylipins / Q000494:pharmacology; D019441:Plant Epidermis / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000201:enzymology; D010941:Plant Tumors / Q000469:parasitology*; D056909:Plasmodiophorida / Q000187:drug effects / Q000502:physiology*; D011725:Pyridines / Q000494:pharmacology
chemical_list: D000596:Amino Acids; D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D003979:Diazonium Compounds; D054883:Oxylipins; D011725:Pyridines; C532883:jasmonoyl-isoleucine; D007532:Isoleucine; C056727:2-(2'-pyridyldithio)benzyldiazoacetate; C011006:jasmonic acid; C532184:ARGAH2 protein, Arabidopsis; D000581:Amidohydrolases