title: Embryonic diapause is conserved across mammals.
abstract: Embryonic diapause (ED) is a temporary arrest of embryo development and is characterized by delayed implantation in the uterus. ED occurs in blastocysts of less than 2% of mammalian species, including the mouse (Mus musculus). If ED were an evolutionarily conserved phenomenon, then it should be inducible in blastocysts of normally non-diapausing mammals, such as domestic species. To prove this hypothesis, we examined whether blastocysts from domestic sheep (Ovis aries) could enter into diapause following their transfer into mouse uteri in which diapause conditions were induced. Sheep blastocysts entered into diapause, as demonstrated by growth arrest, viability maintenance and their ED-specific pattern of gene expression. Seven days after transfer, diapausing ovine blastocysts were able to resume growth in vitro and, after transfer to surrogate ewe recipients, to develop into normal lambs. The finding that non-diapausing ovine embryos can enter into diapause implies that this phenomenon is phylogenetically conserved and not secondarily acquired by embryos of diapausing species. Our study questions the current model of independent evolution of ED in different mammalian orders.
authors.affiliation: Department of Comparative Biomedical Sciences, University of Teramo, Teramo, Italy. gptak@unite.it
authors.forename: Grazyna E
authors.initials: GE
authors.lastname: Ptak
authors.forename: Emanuela
authors.initials: E
authors.lastname: Tacconi
authors.forename: Marta
authors.initials: M
authors.lastname: Czernik
authors.forename: Paola
authors.initials: P
authors.lastname: Toschi
authors.forename: Jacek A
authors.initials: JA
authors.lastname: Modlinski
authors.forename: Pasqualino
authors.initials: P
authors.lastname: Loi
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D017931:DNA Primers / Q000235:genetics; D010064:Embryo Implantation / Q000502:physiology*; D047108:Embryonic Development / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D051379:Mice; D008954:Models, Biological; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012756:Sheep; D018709:Statistics, Nonparametric
chemical_list: D017931:DNA Primers