title: Effect of alloxan on spontaneous lipolysis and glutathione system in isolated rat adipocytes.
abstract: In vitro experiments on cultured adipocytes from epididymal adipose tissue showed that addition of alloxan (0.5-10.0 mmol/liter) to the incubation medium induced the development of oxidative stress accompanied by an increase in the concentration of reactive oxygen species, TBA-reactive substances, and lipid hydroperoxides in cells. The redox state of adipocytes changed significantly under these conditions, which was associated with a decrease in the amount of reduced tripeptide, an increase in the content of glutathione disulfide, and a decrease in the reduced/oxidized glutathione ratio. The development of oxidative stress in adipocytes was accompanied by activation of spontaneous lipolysis, which probably plays an important role in the mechanisms of insulin resistance.
authors.affiliation: Department of Biochemistry and Molecular Biology, Siberian State Medical University, Federal Agency of Health Care and Social Development, Tomsk, Russia.
authors.forename: V V
authors.initials: VV
authors.lastname: Ivanov
authors.forename: E V
authors.initials: EV
authors.lastname: Shakhristova
authors.forename: E A
authors.initials: EA
authors.lastname: Stepovaya
authors.forename: T V
authors.initials: TV
authors.lastname: Zhavoronok
authors.forename: V V
authors.initials: VV
authors.lastname: Novitsky
mesh_terms: D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism*; D000496:Alloxan / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D003920:Diabetes Mellitus / Q000378:metabolism; D005978:Glutathione / Q000096:biosynthesis*; D019803:Glutathione Disulfide / Q000096:biosynthesis; D007333:Insulin Resistance; D008066:Lipolysis / Q000187:drug effects*; D008297:Male; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D000496:Alloxan; D005978:Glutathione; D019803:Glutathione Disulfide