title: Protein oriented ligation on nanoparticles exploiting O6-alkylguanine-DNA transferase (SNAP) genetically encoded fusion.
abstract: A bimodular genetic fusion comprising a delivery module (scFv) and a capture module (SNAP) is proposed as a novel strategy for the site-specific covalent conjugation of targeting peptides to nanoparticles. An scFv mutant selective for HER2 tumor antigen is chosen as the targeting ligand. SNAP-scFv is immobilized on magnetofluorescent nanoparticles and its targeting efficiency against HER2-positive cells is assessed by flow cytometry and immunofluorescence.
authors.affiliation: Dipartimento di Biotecnologie e Bioscienze, Università di Milano-Bicocca, Piazza della Scienza 2, Milan, Italy.
authors.forename: Miriam
authors.initials: M
authors.lastname: Colombo
authors.forename: Serena
authors.initials: S
authors.lastname: Mazzucchelli
authors.forename: Josè Maria
authors.initials: JM
authors.lastname: Montenegro
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Galbiati
authors.forename: Fabio
authors.initials: F
authors.lastname: Corsi
authors.forename: Wolfgang J
authors.initials: WJ
authors.lastname: Parak
authors.forename: Davide
authors.initials: D
authors.lastname: Prosperi
mesh_terms: D016923:Cell Death; D045744:Cell Line, Tumor; D006801:Humans; D055767:Immobilized Proteins / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry*; D019853:O(6)-Methylguanine-DNA Methyltransferase / Q000235:genetics* / Q000378:metabolism*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism*; D057127:Single-Chain Antibodies / Q000378:metabolism
chemical_list: D055767:Immobilized Proteins; D019853:O(6)-Methylguanine-DNA Methyltransferase; D018719:Erb-b2 Receptor Tyrosine Kinases; D011993:Recombinant Fusion Proteins; D057127:Single-Chain Antibodies; C508053:ERBB2 protein, human