title: The frpB1 gene of Helicobacter pylori is regulated by iron and encodes a membrane protein capable of binding haem and haemoglobin.
abstract: FrpB1 is a novel membrane protein of Helicobacter pylori that is capable of binding both haem and haemoglobin but consistently shows more affinity for haem. The mRNA levels of frpB1 were repressed by iron and lightly modulated by haem or haemoglobin. The overexpression of the frpB1 gene supported cellular growth when haem or haemoglobin were supplied as the only iron source. Three-dimensional modelling revealed the presence of motifs necessary to bind either haem or haemoglobin. Our overall results support the idea that FrpB1 is a membrane protein of H. pylori that allows this pathogen to survive in the human stomach.
authors.affiliation: Posgrado en Ciencias Genómicas, Universidad Autónoma de la Ciudad de México, San Lorenzo 290, Del Valle, México DF, Mexico.
authors.forename: Miguel Angel
authors.initials: MA
authors.lastname: Carrizo-Chávez
authors.forename: Areli
authors.initials: A
authors.lastname: Cruz-Castañeda
authors.forename: José de Jesús
authors.initials: Jde J
authors.lastname: Olivares-Trejo
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D016480:Helicobacter pylori / Q000235:genetics* / Q000378:metabolism*; D006418:Heme / Q000378:metabolism*; D006454:Hemoglobins / Q000378:metabolism*; D006801:Humans; D007501:Iron / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D001425:Bacterial Outer Membrane Proteins; C093120:FrpB protein, bacteria; D006454:Hemoglobins; D006418:Heme; D007501:Iron