title: Assessment of DNA damage in human bone marrow cells and multipotent mesenchymal stromal cells.
abstract: We carried out a comparative analysis of DNA damage (percentage of DNA in comet tail) and frequencies of comets in apoptotic cells in BM samples and cultures of BM multipotent mesenchymal stromal cells at different terms of culturing (passages 3-11). The levels of DNA damage in mesenchymal stromal cells remained unchanged during culturing (3.5 ± 0.9 and 4.4 ± 1.2%) and did not differ from those in BM cells (3.6 ± 0.8%). In BM samples, 10-28% atypical cells with high level of DNA damage were detected. In mesenchymal stromal cells, 2.8 ± 0.9 and 3.6 ± 1.8% apoptotic cells were detected at early and late passages, respectively.
authors.affiliation: Medical Genetic Research Center, Russian Academy of Medical Sciences, Moscow, Russia. vanikitina@mail.ru
authors.forename: V A
authors.initials: VA
authors.lastname: Nikitina
authors.forename: A I
authors.initials: AI
authors.lastname: Chausheva
authors.forename: A K
authors.initials: AK
authors.lastname: Zhanataev
authors.forename: E Yu
authors.initials: EY
authors.lastname: Osipova
authors.forename: A D
authors.initials: AD
authors.lastname: Durnev
authors.forename: N P
authors.initials: NP
authors.lastname: Bochkov
mesh_terms: D001854:Bone Marrow Cells / Q000648:ultrastructure*; D002478:Cells, Cultured; D004249:DNA Damage; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000648:ultrastructure*; D039902:Multipotent Stem Cells / Q000648:ultrastructure*; D017154:Stromal Cells / Q000648:ultrastructure*