title: Super-paramagnetic iron oxide nanoparticles for use in extrapulmonary tuberculosis diagnosis.
abstract: The limited sensitivity of serological tests for mycobacterial antigens has encouraged the development of a nanoparticle probe specific for the extrapulmonary form of Mycobacterium tuberculosis (Mtb). We developed an innovative probe comprised of super-paramagnetic iron oxide (SPIO) nanoparticles conjugated with Mtb surface antibody (MtbsAb-nanoparticles) to provide ultrasensitive imaging of biomarkers involved in extrapulmonary Mtb infection. MtbsAb-nanoparticles were significantly conjugated with Mtb bacilli. The extent of contrast enhancement reduction on magnetic resonance imaging (MRI) for Mtb and human monocytic THP1 cells was proportional to the concentration of MtbsAb-nanoparticles. When MtbsAb-nanoparticles were intravenously injected into mice bearing Mtb granulomas, the granulomatous site showed a 14-fold greater reduction in signal intensity enhancement on T(2) -weighted MR images compared with an opposing site that received PBS injection. Mtb sAb-nanoparticles represent a new non-invasive technology for the diagnosis of extrapulmonary Mtb.
authors.affiliation: Department of Pulmonary and Critical Care Medicine, Taipei Medical University-Shuang Ho Hospital, Taipei, Taiwan.
authors.forename: C-N
authors.initials: CN
authors.lastname: Lee
authors.forename: Y-M
authors.initials: YM
authors.lastname: Wang
authors.forename: W-F
authors.initials: WF
authors.lastname: Lai
authors.forename: T-J
authors.initials: TJ
authors.lastname: Chen
authors.forename: M-C
authors.initials: MC
authors.lastname: Yu
authors.forename: C-L
authors.initials: CL
authors.lastname: Fang
authors.forename: F-L
authors.initials: FL
authors.lastname: Yu
authors.forename: Y-H
authors.initials: YH
authors.lastname: Tsai
authors.forename: W H-S
authors.initials: WH
authors.lastname: Chang
authors.forename: C S
authors.initials: CS
authors.lastname: Zuo
authors.forename: P F
authors.initials: PF
authors.lastname: Renshaw
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial; D005290:Ferric Compounds; D008279:Magnetic Resonance Imaging / Q000379:methods; D051379:Mice; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D053758:Nanoparticles; D014376:Tuberculosis / Q000175:diagnosis*
chemical_list: D000907:Antibodies, Bacterial; D005290:Ferric Compounds; C000499:ferric oxide