title: Development of a multiplex polymerase chain reaction for simultaneous detection of wheat viruses and a phytoplasma in China.
abstract: Barley yellow dwarf viruses (BYDVs, mainly consisting of three strains, GAV, GPV and PAV, in China), barley stripe mosaic virus (BSMV), wheat yellow mosaic virus (WYMV), wheat dwarf virus (WDV) and wheat blue dwarf phytoplasma (WBD) constitute a group of major wheat pathogens that have caused huge yield losses but are scarcely distinguished by their phenotype alone. For the simultaneous detection and discrimination of these seven pathogens in wheat, a multiplex polymerase chain reaction (M-PCR) method was developed in this study through a series of parameter optimizations. Detection and sensitivity tests using samples collected from the field indicate that the M-PCR method can rapidly, simultaneously and relatively effectively detect BYDV-GAV, -GPV, -PAV, BSMV, WYMV, WDV and WBD.
authors.affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas, Key Laboratory of Crop Pest Integrated Pest Management on the Loess Plateau of Ministry of Agriculture, Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, College of Plant Protection, Northwest A&F University, Yangling, 712100 Shaanxi, P. R. China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Tao
authors.forename: Jianyun
authors.initials: J
authors.lastname: Man
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Wu
mesh_terms: D002681:China; D003001:Cloning, Molecular; D017931:DNA Primers / Q000592:standards; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D045582:Phytoplasma / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010935:Plant Diseases / Q000382:microbiology* / Q000821:virology; D010942:Plant Viruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D014908:Triticum / Q000382:microbiology* / Q000821:virology*
chemical_list: D017931:DNA Primers