title: A method for determining alkaline phosphatase activity in marine phytoplankton using spectrofluorometry.
abstract: A method for determining relative percent intensity alkaline phosphatase activity (APA) using enzyme labeled fluorescence coupled with spectrofluorometry is presented. Compared to traditional microscopy and flow cytometry, we increase statistical power and reduce sample-handling issues. Combined with a biological standard, our method can quantify APA of natural plankton assemblages.
authors.affiliation: Department of Ocean Sciences, University of California Santa Cruz, CA 95064, USA. mdbpeacock@gmail.com
authors.forename: Melissa Blakely
authors.initials: MB
authors.lastname: Peacock
authors.forename: Raphael M
authors.initials: RM
authors.lastname: Kudela
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis*; D010839:Phytoplankton / Q000201:enzymology*; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D000469:Alkaline Phosphatase