title: Effects of sinensetin on lipid metabolism in mature 3T3-L1 adipocytes.
abstract: Sinensetin is a rare polymethoxylated flavone found in certain citrus fruits. In this study, we investigated the effects of sinensetin on lipid metabolism in mature 3T3-L1 adipocytes. Sinensetin decreased the expression of sterol regulatory element-binding protein 1c (SREBP1c), suggesting its antiadipogeneic property via downreguation of SREBP1c. Also, sinensetin increased the phosphorylation of protein kinase A and hormone-sensitive lipase, indicating its lipolytic property via a cAMP-mediated signaling pathway. Moreover, sinensetin inhibited insulin-stimulated glucose uptake by decreasing the phosphorylation of insulin receptor substrate and Akt. Furthermore, sinensetin increased the phosphorylation of AMP-activated protein kinase (AMPK) and acetyl-CoA carboxylase. It also upregulated mRNA expression of carnitine palmitoyltransferase-1a, suggesting that sinensetin enhances fatty acid β-oxidation through the AMPK pathway. Taken together, these results suggest that sinensetin may have potential as a natural agent for prevention/improvement of obesity.
authors.affiliation: Department of Biology, Jeju National University, 102 Jejudaehakno, Jejusi, Jeju Special Self-Governing Province 690-756, Korea.
authors.forename: Seong-Il
authors.initials: SI
authors.lastname: Kang
authors.forename: Hye-Sun
authors.initials: HS
authors.lastname: Shin
authors.forename: Hee-Chul
authors.initials: HC
authors.lastname: Ko
authors.forename: Se-Jae
authors.initials: SJ
authors.lastname: Kim
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism*; D000818:Animals; D005419:Flavonoids / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects*; D008066:Lipolysis; D051379:Mice; D010766:Phosphorylation; D051780:Sterol Regulatory Element Binding Protein 1 / Q000378:metabolism
chemical_list: D005419:Flavonoids; C497388:Srebf1 protein, mouse; D051780:Sterol Regulatory Element Binding Protein 1; C059295:sinensetin; D005947:Glucose