title: Formation of dAMP-glycerol and dAMP-Tris derivatives by Thermococcus kodakaraensis DNA primase.
abstract: In the presence of dATP, glycerol, and Tris buffer, the DNA primase isolated from Thermococcus kodakaraensis catalyzed the formation of dAMP and two products that were identified as dAMP-glycerol and dAMP-Tris. These products were formed by the T. kodakaraensis p41 catalytic subunit alone and the T. kodakaraensis p41-p46 complex in the absence of a DNA template. They were not formed with preparations containing the catalytically inactive p41 subunit. Similar glycerol and Tris derivatives as well as dNMPs were also formed with dGTP, dCTP, or dTTP. The mechanism contributing to the formation of these products and its implications in the initiation reaction catalyzed by the T. kodakaraensis primase are discussed.
authors.affiliation: Program of Molecular Biology, 5 Memorial Sloan-Kettering Cancer Center, New York, New York 1006, USA.
authors.forename: Wiebke
authors.initials: W
authors.lastname: Chemnitz Galal
authors.forename: Miao
authors.initials: M
authors.lastname: Pan
authors.forename: Gary
authors.initials: G
authors.lastname: Giulian
authors.forename: Wei
authors.initials: W
authors.lastname: Yuan
authors.forename: Shuwei
authors.initials: S
authors.lastname: Li
authors.forename: James L
authors.initials: JL
authors.lastname: Edwards
authors.forename: John P
authors.initials: JP
authors.lastname: Marino
authors.forename: Zvi
authors.initials: Z
authors.lastname: Kelman
authors.forename: Jerard
authors.initials: J
authors.lastname: Hurwitz
mesh_terms: D019843:Archaeal Proteins / Q000737:chemistry; D002384:Catalysis; D019915:DNA Primase / Q000737:chemistry*; D003838:Deoxyadenine Nucleotides / Q000737:chemistry*; D005990:Glycerol / Q000737:chemistry*; D019712:Thermococcus / Q000201:enzymology*; D014325:Tromethamine / Q000737:chemistry*
chemical_list: D019843:Archaeal Proteins; D003838:Deoxyadenine Nucleotides; D014325:Tromethamine; C012873:2'-deoxy-5'-adenosine monophosphate; D019915:DNA Primase; C026600:2'-deoxyadenosine triphosphate; D005990:Glycerol