title: Characterization of the human papillomavirus (HPV) integration sites into genital cancers.
abstract: Oncogenic HPVs have been found frequently integrated into human genome of invasive cancers and chromosomal localization has been extensively investigated in cervical carcinoma. Few studies have analyzed the HPV integration loci in other genital cancers. We have characterized the integration sites of HPV16 in invasive penile carcinoma by means of Alu-HPV-based PCR. Nucleotide sequence analysis of viral-human DNA junctions showed that HPV integration occurred in one case within the chromosome 8q21.3 region, in which the FAM92A1 gene is mapped, and in the second case inside the chromosome 16p13.3, within the intronic region of TRAP1 gene. These results confirm previous observations, summarized in a systematic review of the literature, on the HPV integration events in gene loci relevant to cancer pathogenesis.
authors.affiliation: Molecular Biology and Viral Oncology and AIDS Reference Centre, National Cancer Institute Fondazione Pascale, Cappella Cangiani, 80131, Naples, Italy.
authors.forename: Clorinda
authors.initials: C
authors.lastname: Annunziata
authors.forename: Luigi
authors.initials: L
authors.lastname: Buonaguro
authors.forename: Franco M
authors.initials: FM
authors.lastname: Buonaguro
authors.forename: Maria Lina
authors.initials: ML
authors.lastname: Tornesello
mesh_terms: D001483:Base Sequence; D005260:Female; D005833:Genital Neoplasms, Female / Q000235:genetics* / Q000821:virology*; D054884:Host-Pathogen Interactions / Q000235:genetics; D052162:Human papillomavirus 16 / Q000235:genetics*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D030361:Papillomavirus Infections / Q000235:genetics / Q000821:virology; D010412:Penile Neoplasms / Q000235:genetics* / Q000821:virology*; D016662:Virus Integration / Q000235:genetics*