title: Carbonate-dissolving bacteria from 'miliolite', a bioclastic limestone, from Gopnath, Gujarat, Western India.
abstract: In the present investigation, the abundance and molecular phylogeny of part of the culturable bacterial population involved in the dissolution of "miliolite", a bioclastic limestone, from Gopnath, India, was studied. Carbonate-dissolving bacteria were isolated, enumerated and screened for their ability to dissolve miliolite. Amplified ribosomal DNA restriction analysis (ARDRA) indicated 14 operational taxonomic units (OTUs) to be distributed in 5 different clades at a similarity coefficient of 0.85. Then, 16S rRNA sequence analysis helped to decipher that the majority of carbonate-dissolving bacteria were affiliated to phyla Firmicutes (Families Bacillaceae and Staphylococcaceae) and Actinobacteria (Family Promicromonosporaceae) indicating their role in miliolite weathering.
authors.affiliation: Department of Microbiology & Biotechnology Centre, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara-390002, Gujarat, India.
authors.forename: Gangavarapu
authors.initials: G
authors.lastname: Subrahmanyam
authors.forename: Ravi
authors.initials: R
authors.lastname: Vaghela
authors.forename: Nilesh Pinakinprasad
authors.initials: NP
authors.lastname: Bhatt
authors.forename: Gattupalli
authors.initials: G
authors.lastname: Archana
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000302:isolation & purification* / Q000378:metabolism; D002119:Calcium Carbonate; D002254:Carbonates / Q000378:metabolism*; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004783:Environmental Microbiology; D007194:India; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D002254:Carbonates; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D002119:Calcium Carbonate