title: Comprehensive HILIC × RPLC with mass spectrometry detection for the analysis of saponins in Panax notoginseng.
abstract: A comprehensive off-line two-dimensional liquid chromatography (2D-LC) method coupling hydrophilic interaction liquid chromatography (HILIC) and reversed phase liquid chromatography (RPLC) was developed in this study to detect as many saponins as possible in extracts of Panax notoginseng. The orthogonality of the 2D HILIC × RPLC was up to 81%, and the peak capacity was 10200. In total, 224 saponins were found, and some of them were trace amounts. Besides, a screening table designed by adding molecular weights of possible aglycones and sugars was constructed to help rapidly characterize the saponins using MS information. Unfortunately, the structure of saponins could not be identified by using only MS information.
authors.affiliation: Engineering Research Center of Pharmaceutical Process Chemistry, Ministry of Education, School of Pharmacy, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Xing
authors.forename: Tu
authors.initials: T
authors.lastname: Liang
authors.forename: Guobin
authors.initials: G
authors.lastname: Shen
authors.forename: Xinliang
authors.initials: X
authors.lastname: Wang
authors.forename: Yu
authors.initials: Y
authors.lastname: Jin
authors.forename: Xinmiao
authors.initials: X
authors.lastname: Liang
mesh_terms: D053000:Analytic Sample Preparation Methods; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D013058:Mass Spectrometry / Q000379:methods*; D053723:Panax notoginseng / Q000737:chemistry*; D012503:Saponins / Q000032:analysis* / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D004365:Drugs, Chinese Herbal; D012503:Saponins