title: Cryopreservation of in vitro grown shoot tips of Diospyros kaki thunb. using different methods.
abstract: The objective of this study was to compare the potential of different cryopreservation strategies for in vitro shoot tips of Diospyros kaki Thunb. The treatments consisted of three different cryopreservation methods: vitrification, droplet-vitrification and modified droplet-vitrification. The following variables were assessed: cold acclimation, sucrose concentration in the preculture medium and PVS2 treatment time. A higher average survival level was obtained using the modified droplet-vitrification method compared to the other two methods.
authors.affiliation: Key Laboratory of Horticultural Plant Biology, Affiliated to Ministry of Education, Huazhong Agricultural University, Wuhan, China.
authors.forename: Y L
authors.initials: YL
authors.lastname: Niu
authors.forename: Z R
authors.initials: ZR
authors.lastname: Luo
authors.forename: Y F
authors.initials: YF
authors.lastname: Zhang
authors.forename: Q L
authors.initials: QL
authors.lastname: Zhang
mesh_terms: D000222:Adaptation, Physiological; D003080:Cold Temperature; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents; D003470:Culture Media; D004121:Dimethyl Sulfoxide; D029788:Diospyros / Q000166:cytology* / Q000502:physiology; D019855:Ethylene Glycol; D055993:Germ Cells, Plant / Q000166:cytology* / Q000502:physiology; D005990:Glycerol; D018520:Plant Shoots / Q000254:growth & development*; D058439:Plant Somatic Embryogenesis Techniques; D012038:Regeneration; D013395:Sucrose; D058989:Vitrification
chemical_list: D003451:Cryoprotective Agents; D003470:Culture Media; D013395:Sucrose; D019855:Ethylene Glycol; D005990:Glycerol; D004121:Dimethyl Sulfoxide