title: Novel protein extraction approach using micro-sized chamber for evaluation of proteins eluted from formalin-fixed paraffin-embedded tissue sections.
abstract: We describe a novel antigen-retrieval method using a micro-sized chamber for mass spectrometry (MS) analysis to identify proteins that are preferentially eluted from formalin-fixed paraffin-embedded (FFPE) samples. This approach revealed that heat-induced antigen retrieval (HIAR) from an FFPE sample fixed on a glass slide not only improves protein identification, but also facilitates preferential elution of protein subsets corresponding to the properties of antigen-retrieval buffers. Our approach may contribute to an understanding of the mechanism of HIAR.
authors.affiliation: Medical Genetics Division, Shizuoka Cancer Center Research Institute, 1007 Shimonagakubo, Nagaizumi-cho, Sunto-gun, Shizuoka 411-8777, Japan. k.hatakeyama@scchr.jp.
authors.forename: Keiichi
authors.initials: K
authors.lastname: Hatakeyama
authors.forename: Kanako
authors.initials: K
authors.lastname: Wakabayashi-Nakao
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Aoki
authors.forename: Shun-Ichiro
authors.initials: S
authors.lastname: Ogura
authors.forename: Ken
authors.initials: K
authors.lastname: Yamaguchi
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakajima
authors.forename: Taka-Aki
authors.initials: TA
authors.lastname: Sato
authors.forename: Tohru
authors.initials: T
authors.lastname: Mochizuki
authors.forename: Isamu
authors.initials: I
authors.lastname: Hayashi