title: Hepatoprotective potential of ethanolic extract of Ziziphus oenoplia (L.) Mill roots against antitubercular drugs induced hepatotoxicity in experimental models.
abstract: OBJECTIVE
  To evaluate the hepatoprotective potential of ethanolic (50%) extract of Ziziphus oenoplia (L.) Mill (Z. oenoplia) root against isoniazid (INH) and rifampicin (RIF) induced liver damage in animal models.
  
  
  METHODS
  Five groups of six rats each were selected for the study. Ethanolic extract at a dose of 150 and 300 mg/kg as well as silymarin (100 mg/kg) were administered orally once daily for 21 d in INH + RIF treated groups. The serum levels of glutamic oxaloacetic transaminase (SGOT), glutamate pyruvate transaminase (SGPT), alkaline phosphatase (SALP), and bilirubin were estimated along with activities of superoxide dismutase, catalase, glutathione S-transferase, glutathione peroxidase, and hepatic melondialdehyde formation. Histopathological analysis was carried out to assess injury to the liver.
  
  
  RESULTS
  The considerably elevated serum enzymatic activities of glutamic oxaloacetic transaminase, glutamate pyruvate transaminase, alkaline phosphatase and bilirubin due to INH + RIF treatment were restored towards normal in a dose dependent manner after the treatment with ethanolic extract of Z. oenoplia roots. Meanwhile, the decreased activities of superoxide dismutase, catalase, glutathione S-transferase and glutathione peroxidase were also restored towards normal dose dependently. In addition, ethanolic extract also significantly prevented the elevation of hepatic melondialdehyde formation in the liver of INH + RIF intoxicated rats in a dose dependent manner. The biochemical observations were supplemented with histopathological examination of rat liver sections.
  
  
  CONCLUSIONS
  The results of this study strongly indicate that ethanolic extract of Z. oenoplia has a potent hepatoprotective action against INH + RIF induced hepatic damage in rats.
authors.affiliation: Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute (Council of Scientific and Industrial Research), Rana Pratap Marg, P.O. Box No. 436, Lucknow 226001, Uttar Pradesh, India. chvrao72@yahoo.com
authors.forename: Ch V
authors.initials: ChV
authors.lastname: Rao
authors.forename: A K S
authors.initials: AK
authors.lastname: Rawat
authors.forename: Anil P
authors.initials: AP
authors.lastname: Singh
authors.forename: Arpita
authors.initials: A
authors.lastname: Singh
authors.forename: Neeraj
authors.initials: N
authors.lastname: Verma
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D000995:Antitubercular Agents / Q000633:toxicity*; D001663:Bilirubin / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology / Q000517:prevention & control*; D004798:Enzymes / Q000378:metabolism; D000431:Ethanol / Q000494:pharmacology; D022781:Hepatocytes / Q000187:drug effects; D007538:Isoniazid / Q000633:toxicity; D008297:Male; D008517:Phytotherapy / Q000379:methods; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots; D051381:Rats; D017208:Rats, Wistar; D012293:Rifampin / Q000633:toxicity; D031957:Ziziphus
chemical_list: D000975:Antioxidants; D000995:Antitubercular Agents; D004798:Enzymes; D010936:Plant Extracts; D000431:Ethanol; D001663:Bilirubin; D007538:Isoniazid; D012293:Rifampin