title: Quantitative PCR and in vivo efficacy of antibiotics in the treatment of Vibrio vulnificus infection in a mouse model.
abstract: The Centers for Disease Control and Prevention (CDC) recommend oral or intravenous doxycycline plus a third-generation cephalosporin or fluoroquinolone alone for the treatment of Vibrio vulnificus infections. Until now, no study has compared oral with parenteral administered doxycycline with respect to their in vivo efficacy. In the present work, ICR mice infected with a high dose of V. vulnificus were administered ciprofloxacin, ceftriaxone, and doxycycline. The bacterial DNA copy number in surviving and non-surviving mice was determined using quantitative polymerase chain reaction (qPCR). In this setting, ciprofloxacin was the most effective monotherapeutic drug, but a higher survival rate (50%) was achieved using the combination therapy of intraperitoneal doxycycline plus ceftriaxone. The blood of non-surviving mice at 12 h post-infection contained at least 10(4) DNA copies/μL, in contrast to 10(2) to 10(3) DNA copies/μL in surviving mice. Thus, in the treatment of V. vulnificus infections in humans, when the intravenous form of doxycycline is unavailable, ciprofloxacin might be a better option than oral doxycycline to lower mortality. In addition, our results demonstrate that qPCR can be a useful tool for identifying the V. vulnificus load in infected patients, with the DNA copy number providing a marker of either disease severity or mortality.
authors.affiliation: Division of Infectious Diseases, Department of Internal Medicine, Chosun University School of Medicine, 588 Seosuk-dong, Dong-gu, Gwangju, 501-717, Republic of Korea.
authors.forename: G P
authors.initials: GP
authors.lastname: Neupane
authors.forename: D-M
authors.initials: DM
authors.lastname: Kim
authors.forename: N R
authors.initials: NR
authors.lastname: Yun
authors.forename: S-H
authors.initials: SH
authors.lastname: Shin
authors.forename: S-C
authors.initials: SC
authors.lastname: Lim
authors.forename: C-H
authors.initials: CH
authors.lastname: Choi
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D058491:Bacterial Load; D001431:Bacteriological Techniques / Q000379:methods*; D001769:Blood / Q000382:microbiology; D002443:Ceftriaxone / Q000008:administration & dosage; D002939:Ciprofloxacin / Q000008:administration & dosage; D004195:Disease Models, Animal; D004318:Doxycycline / Q000008:administration & dosage; D016903:Drug Monitoring / Q000379:methods*; D004359:Drug Therapy, Combination / Q000379:methods; D008297:Male; D051379:Mice; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D016019:Survival Analysis; D016896:Treatment Outcome; D014735:Vibrio Infections / Q000188:drug therapy* / Q000382:microbiology; D041261:Vibrio vulnificus / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D000900:Anti-Bacterial Agents; D002939:Ciprofloxacin; D002443:Ceftriaxone; D004318:Doxycycline