title: Crystallization and preliminary X-ray diffraction characterization of the XccFimX(EAL)-c-di-GMP and XccFimX(EAL)-c-di-GMP-XccPilZ complexes from Xanthomonas campestris.
abstract: c-di-GMP is a major secondary-messenger molecule in regulation of bacterial pathogenesis. Therefore, the c-di-GMP-mediated signal transduction network is of considerable interest. The PilZ domain was the first c-di-GMP receptor to be predicted and identified. However, every PilZ domain binds c-di-GMP with a different binding affinity. Intriguingly, a noncanonical PilZ domain has recently been found to serve as a mediator to link FimX(EAL) to the PilB or PilT ATPase to control the function of type IV pili (T4P). It is thus essential to determine the structure of the FimX(EAL)-PilZ complex in order to determine how the binding of c-di-GMP to the FimX(EAL) domain induces conformational change of the adjoining noncanonical PilZ domain, which may transmit information to PilB or PilT to control T4P function. Here, the preparation and preliminary X-ray diffraction studies of the XccFimX(EAL)-c-di-GMP and XccFimX(EAL)-c-di-GMP-XccPilZ complexes from Xcc (Xanthomonas campestris pv. campesteris) are reported. Detailed studies of these complexes may allow a more thorough understanding of how c-di-GMP transmits its effects through the degenerate EAL domain and the noncanonical PilZ domain.
authors.affiliation: Institute of Biochemistry, National Chung Hsing University, Taichung 40227, Taiwan.
authors.forename: Yi-Ting
authors.initials: YT
authors.lastname: Liao
authors.forename: Ko-Hsin
authors.initials: KH
authors.lastname: Chin
authors.forename: Wei-Ting
authors.initials: WT
authors.lastname: Kuo
authors.forename: Mary Lay-Cheng
authors.initials: ML
authors.lastname: Chuah
authors.forename: Zhao-Xun
authors.initials: ZX
authors.lastname: Liang
authors.forename: Shan-Ho
authors.initials: SH
authors.lastname: Chou
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D006152:Cyclic GMP / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D016959:Xanthomonas campestris / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; C062025:bis(3',5')-cyclic diguanylic acid; D006152:Cyclic GMP