title: Biosynthesis of iminodiacetic acid from iminodiacetonitrile by immobilized recombinant Escherichia coli harboring nitrilase.
abstract: Iminodiacetic acid (IDA) is widely used as an intermediate in the manufacture of chelating agents, glyphosate herbicides and surfactants. In the current work, the fragment with the length of 1,110 bp encoding the Acidovorax facilis nitrilase was obtained. The recombinant nitrilase expressed in Escherichia coli BL21 (DE3) was successfully used in the production of IDA from iminodiacetonitrile. To improve the stability of operation, the recombinant cells were entrapped in polyvinyl alcohol (PVA) and sodium alginate (SA) copolymer. The maximum relative nitrilase activity with 98.1% was further observed at 1.0% SA, 8.0% PVA, 1.0% CaCl(2), and 5.0% wet cells, under conditions of 1.0% iminodiacetonitrile in distilled water and a temperature of 40°C, respectively. The entrapped cells facilitated easy separation and good recycling compared with free cells. Moreover, the immobilized cells showed good operation and storage stability. This report is the first to describe IDA preparation using immobilized recombinant E. coli harboring nitrilase.
authors.affiliation: Institute of Bioengineering, Zhejiang University of Technology, Hangzhou, China.
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Liu
authors.forename: Ming
authors.initials: M
authors.lastname: Zhou
authors.forename: Xin-Hong
authors.initials: XH
authors.lastname: Zhang
authors.forename: Jian-Miao
authors.initials: JM
authors.lastname: Xu
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Xue
authors.forename: Yu-Guo
authors.initials: YG
authors.lastname: Zheng
mesh_terms: D000097:Acetonitriles / Q000378:metabolism*; D000464:Alginates / Q000737:chemistry; D000619:Aminohydrolases / Q000235:genetics / Q000378:metabolism*; D018914:Cells, Immobilized; D042621:Comamonadaceae / Q000201:enzymology / Q000235:genetics; D015870:Gene Expression; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D007098:Imino Acids / Q000378:metabolism*; D011142:Polyvinyl Alcohol / Q000737:chemistry; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013696:Temperature
chemical_list: D000097:Acetonitriles; D000464:Alginates; D006603:Hexuronic Acids; D007098:Imino Acids; D011994:Recombinant Proteins; D020723:Glucuronic Acid; D011142:Polyvinyl Alcohol; D000619:Aminohydrolases; C021867:nitrilase; C008109:iminodiacetic acid