title: The role of AMPKα in high-glucose-induced dysfunction of cultured rat mesangial cells.
abstract: OBJECTIVE
  Adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK) is known as a mammalian cell energy sensor, which could regulate cellular energy metabolism via sensing the alterations of energy balance, such as oversupply or lack of glucose and fatty acid. Recent studies have suggested that AMPK could also regulate many other biological processes, including cell cycling, inflammation, protein synthesis, and so on. In this study, AMPK signaling in high-glucose-induced dysfunction of mesangial cells (MCs) was investigated.
  
  
  METHODS
  Established rat glomerular MCs were treated under normal glucose (5.6 mM glucose) or high-glucose conditions (30 mM glucose). mRNA levels of AMPK subunits were detected by reverse transcriptase-polymerase chain reaction. Expressions of AMPKα, phosphorylated AMPKα (p-AMPKα), phosphorylated acetyl-CoA carboxylase (p-ACC), and collagen IV were measured by Western blot.
  
  
  RESULTS
  Under high-glucose conditions, AMPKα protein expression and mRNA levels were significantly decreased. High-glucose treatment also induced a notable decrease in p-AMPKα and p-ACC expression. AMPKα activation by 5-aminoimidazole-4-carboxamide-1-β-d-ribofuranoside (AICAR) effectively ameliorated high-glucose-induced dysfunction of MCs, including cell proliferation, cell-cycle progression, and collagen IV production.
  
  
  CONCLUSIONS
  High glucose impaired AMPKα in its expression and activity; AICAR significantly ameliorated high-glucose-induced proliferation of MCs and collagen IV production, indicating a role of AMPKα in high-glucose-induced dysfunction of MCs.
authors.affiliation: Department of Nephrology, Provincial Hospital Affiliated to Shandong University, JiNan, China.
authors.forename: Zhi-Mei
authors.initials: ZM
authors.lastname: Lv
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Peng-Ju
authors.initials: PJ
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.forename: Zhen-Hua
authors.initials: ZH
authors.lastname: Jia
authors.forename: Rong
authors.initials: R
authors.lastname: Wang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wan
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000096:biosynthesis / Q000235:genetics*; D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D003928:Diabetic Nephropathies / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology*; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D005920:Glomerular Mesangium / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D005947:Glucose / Q000633:toxicity*; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013549:Sweetening Agents
chemical_list: D012333:RNA, Messenger; D013549:Sweetening Agents; C543928:Prkaa2 protein, rat; D055372:AMP-Activated Protein Kinases; C568305:Prkaa1 protein, rat; D005947:Glucose