title: Curculigoside isolated from Curculigo orchioides prevents hydrogen peroxide-induced dysfunction and oxidative damage in calvarial osteoblasts.
abstract: Reactive oxygen species (ROS), including H(2)O(2), play a critical role in the pathophysiology of osteoporosis. Therefore, agents or antioxidants that can inhibit ROS production have a high clinical value in the treatment of osteoporosis. Curculigoside (CUR), one of the main bioactive phenolic compounds isolated from the rhizome of Curculigo orchioides Gaertn., is reported to have potent antioxidant and anti-osteoporotic properties. However, there is no direct evidence to link the antioxidant capacity of CUR with the observed anti-osteoporotic effect, and relevant molecular mechanisms remain unclear. Therefore, we investigated the protective effects of CUR against oxidative stress in calvarial osteoblasts and discussed the related mechanisms. It was found that osteoblast viability decreased significantly after 48-h exposure to 400 μM of H(2)O(2), compared with vehicle-treated cells, and the cytotoxic effect of H(2)O(2) was reversed significantly when pretreated with 0.1-10 μM of CUR (P< 0.05). Pretreatment with 0.1-10 μM of CUR decreased ROS production and lipid peroxidation, and increased the activities of antioxidant enzymes, such as superoxide dismutase and glutathione peroxidase in osteoblasts induced by H(2)O(2). In addition, H(2)O(2)-induced reduction of differentiation markers such as alkaline phosphatase, calcium deposition, and Runx2 level was significantly recovered in the presence of CUR. CUR also reversed H(2)O(2)-induced stimulation of extracellular signal-regulated kinase 1/2, and nuclear factor-κB signaling and the inhibition of p38 mitogen-activated protein kinase activation. These results provide new insights into the osteoblast-protective mechanisms of CUR through reducing the production of ROS, suggesting that CUR may be developed as a bio-safe agent for the prevention and treatment of osteoporosis and other bone-related human diseases.
authors.affiliation: School of Pharmacy, Second Military Medical University, Shanghai, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Lu
authors.initials: L
authors.lastname: Zhao
authors.forename: Yin
authors.initials: Y
authors.lastname: Wang
authors.forename: Jinlong
authors.initials: J
authors.lastname: Xu
authors.forename: Yan
authors.initials: Y
authors.lastname: Nie
authors.forename: Yuanhui
authors.initials: Y
authors.lastname: Guo
authors.forename: Yongtao
authors.initials: Y
authors.lastname: Tong
authors.forename: Luping
authors.initials: L
authors.lastname: Qin
authors.forename: Qiaoyan
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001565:Benzoates / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D024042:Collagen Type I / Q000096:biosynthesis; D050659:Core Binding Factor Alpha 1 Subunit / Q000096:biosynthesis; D031408:Curculigo / Q000737:chemistry; D005960:Glucosides / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000494:pharmacology / Q000633:toxicity*; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D053244:Osteoprotegerin / Q000096:biosynthesis; D018384:Oxidative Stress / Q000187:drug effects; D053245:RANK Ligand / Q000096:biosynthesis; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D012886:Skull / Q000166:cytology
chemical_list: D000975:Antioxidants; D001565:Benzoates; D024042:Collagen Type I; D050659:Core Binding Factor Alpha 1 Subunit; D005960:Glucosides; D053244:Osteoprotegerin; D053245:RANK Ligand; D017382:Reactive Oxygen Species; C493717:Runx2 protein, rat; C506032:Tnfrsf11b protein, rat; C477170:curculigoside; D006861:Hydrogen Peroxide; D048052:Mitogen-Activated Protein Kinase 3