title: Nanographene-based tyrosinase biosensor for rapid detection of bisphenol A.
abstract: Hydrophilic nanographene (NGP) prepared by ball milling of graphite was used as the support to construct a novel tyrosinase biosensor for determination of bisphenol A (BPA). The performances of the nanographene-based tyrosinase biosensor were systematically compared with those of multiwall carbon nanotubes (MWNTs) modified tyrosinase biosensors. The results indicated that the nanographene-based tyrosinase biosensor provided significant advantages over MWNTs-based tyrosinase biosensor in term of response, repeatability, background current and limit of detection (LOD), which could be attributed to its larger specific surface area and unique hierarchical tyrosinase-NGP nanostructures. The nanographene-based tyrosinase biosensor displayed superior analytical performance over a linear range from 100 nmol L(-1) to 2000 nmol L(-1), with LOD of 33 nmol L(-1) and sensitivity of 3108.4 mA cm(-2)M(-1). The biosensor was further used for detecting BPA (leaching from different vessels) in tap water, and the accuracy of the results was validated by high performance liquid chromatography (HPLC). The nanographene-based tyrosinase biosensor proved to be a promising and reliable tool for rapid detection of BPA leached from polycarbonate plastic products and for on-site rapid analysis of emergency pollution affairs of BPA.
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Lidong
authors.initials: L
authors.lastname: Wu
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Dehui
authors.initials: D
authors.lastname: Deng
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Jin
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China. Electronic address: xianbolu@dicp.ac.cn.
authors.forename: Xianbo
authors.initials: X
authors.lastname: Lu
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China. Electronic address: chenjp@dicp.ac.cn.
authors.forename: Jiping
authors.initials: J
authors.lastname: Chen
mesh_terms: D001559:Benzhydryl Compounds; D015374:Biosensing Techniques / Q000379:methods* / Q000706:statistics & numerical data; D055664:Electrochemical Techniques; D004785:Environmental Pollutants / Q000032:analysis; D004800:Enzymes, Immobilized / Q000737:chemistry; D006108:Graphite; D057230:Limit of Detection; D046529:Microscopy, Electron, Transmission; D008958:Models, Molecular; D014442:Monophenol Monooxygenase / Q000737:chemistry; D053761:Nanocomposites; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure; D037742:Nanotubes, Carbon; D010636:Phenols / Q000032:analysis*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D004785:Environmental Pollutants; D004800:Enzymes, Immobilized; D006108:Graphite; D014442:Monophenol Monooxygenase; D037742:Nanotubes, Carbon; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds