title: Effect of sub-lethal doses of Bacillus thuringiensis subsp. Aizawai and deltamethrin with regard to fertility and organ toxicity in pregnant albino rats.
abstract: Products with Bacillus thuringiensis (Bt) and synthetic insecticides have been widely used against important vectors of human diseases. However, few studies have addressed the application of these substances on the female reproduction apparatus during pregnancy at doses that do not cause clinical symptoms of intoxication. Seventy pregnant albino rats were analyzed with regard to fertility and histopathology of the kidneys, liver and lungs as well as the morphology of the neonates. The rats were submitted to three sub-lethal doses of the biological insecticide XenTari(®) WG (B. thuringiensis subsp. Aizawai) and the synthetic insecticide deltamethrin (Decis(®) 25CE). After the confirmation of copulation, the insecticides were administered orally for either seven days or during the entire pregnancy. The analysis revealed histopathological alterations in all organs analyzed in both treatments. No miscarriages occurred and the neonates did not exhibit signs of malformation of the head, limbs, thorax or abdomen. However, there were a smaller number of pups in the groups that received higher doses of the insecticides in comparison to the control group. Both insecticides produced similar lesions in the kidneys, liver and lungs and reduced the fertility of rats when administered at sub-lethal doses with no clinical signs of intoxication. Thus, this study suggests that sublethal doses of both insecticides can provide chronic toxicity in humans.
authors.affiliation: Department of Animal Morphology and Physiology, University Federal Rural of Pernambuco, Av. Dom Manoel de Medeiros, s/n, Recife, PE, CEP 52171-900, Brazil.
authors.forename: Ana Janaina J M
authors.initials: AJ
authors.lastname: Lemos
authors.forename: Herbert A A
authors.initials: HA
authors.lastname: Siqueira
authors.forename: Valéria
authors.initials: V
authors.lastname: Wanderley-Teixeira
authors.forename: Frederico C L
authors.initials: FC
authors.lastname: Maia
authors.forename: Álvaro A C
authors.initials: ÁA
authors.lastname: Teixeira
authors.forename: Edson J
authors.initials: EJ
authors.lastname: Silva
authors.forename: José V
authors.initials: JV
authors.lastname: Oliveira
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005298:Fertility / Q000187:drug effects*; D007306:Insecticides / Q000633:toxicity*; D007668:Kidney / Q000187:drug effects* / Q000473:pathology; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008168:Lung / Q000187:drug effects* / Q000473:pathology; D009570:Nitriles / Q000633:toxicity*; D009928:Organ Specificity; D011247:Pregnancy; D011498:Protein Precursors / Q000633:toxicity*; D011722:Pyrethrins / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar
chemical_list: C029103:Bacillus thuringiensis protoxin; D001427:Bacterial Toxins; D007306:Insecticides; D009570:Nitriles; D011498:Protein Precursors; D011722:Pyrethrins; C017180:decamethrin