title: Fluorescent function-spacer-lipid construct labelling allows for real-time in vivo imaging of cell migration and behaviour in zebrafish (Danio rerio).
abstract: Real-time in vivo imaging of cell migration and behavior has advanced our understanding of physiological processes in situ, especially in the field of immunology. We carried out the transplantation of a mixed population of blood cells from adult zebrafish (Danio rerio) to 2 day old embryos. The blood cells were treated ex vivo with Function-Spacer-Lipid constructs (FSL) incorporating either fluorescein or Atto488 fluorophores (FSL-FLRO4-I or -II). Excellent labeling efficiency was demonstrated by epifluorescence microscopy and FACScan analysis. Real-time video imaging of the recipient fish showed that the functionality of these cells was retained and not affected by the labeling. The usefulness of FSL-FLRO4-I as a contrast agent in microangiography was explored. Overall, we found both FSL-FLRO4-I and-II promising labeling dyes for real-time in vivo imaging in zebrafish.
authors.affiliation: School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, 1142, New Zealand.
authors.forename: Chuan-Ching
authors.initials: CC
authors.lastname: Lan
authors.forename: Deborah
authors.initials: D
authors.lastname: Blake
authors.forename: Stephen
authors.initials: S
authors.lastname: Henry
authors.forename: Donald R
authors.initials: DR
authors.lastname: Love
mesh_terms: D000818:Animals; D001773:Blood Cells / Q000166:cytology; D002465:Cell Movement; D019793:Fluorescein / Q000737:chemistry / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism*; D008055:Lipids / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D057054:Molecular Imaging / Q000379:methods*; D018919:Neovascularization, Physiologic; D013997:Time Factors; D019737:Transplants; D015027:Zebrafish / Q000502:physiology
chemical_list: D005456:Fluorescent Dyes; D008055:Lipids; D019793:Fluorescein