title: Microtomographic and morphometric  characterization of a bioceramic bone substitute in dental implantology.
abstract: In recent years, bone tissue regeneration studies have led to a deeper knowledge of chemical and structural features of the best biomaterials to be used as replacements for lost bone structures, with the autologus bone still today the only graft material able to ostegenerate, osteinduct and/or osteoconduct. The difficulties of the small available amount of autologus bone, together with morbidity of a second surgical operation on the same patient, have been overcome using both synthetic and biologic substitute bones. The possibility of investigating morphometric characteristics of substitute bones makes it possible to evaluate the predictability of regenerative processes and, so far, a range of different methods have been used for the purpose. X-ray microtomography (micro-CT) is a miniaturized form of conventional tomography, able to analyze the internal structure of small objects, performing three-dimensional images with high spatial resolution (< 10 micron pixel size). For a correct analysis, samples need not be altered or treated in any way, as micro-CT is a non-invasive and non-destructive technique. It shows promising results in biomaterial studies and tissue engineering. This work shows the potential applications of this microtomographic technique by means of an in vitro analysis system, in characterizing morphometric features of human bone tissue, and contributes to the use of this technique in studies concerning biomaterials and bioscaffolds inserted in bone tissue.
authors.affiliation: Dipartimento di Tecnologie e Salute, Istituto Superiore di Sanità, Rome, Italy. deborahhh@tin.it
authors.forename: Deborah
authors.initials: D
authors.lastname: Meleo
authors.forename: Rossella
authors.initials: R
authors.lastname: Bedini
authors.forename: Raffaella
authors.initials: R
authors.lastname: Pecci
authors.forename: Francesca
authors.initials: F
authors.lastname: Mangione
authors.forename: Luciano
authors.initials: L
authors.lastname: Pacifici
mesh_terms: D000328:Adult; D001672:Biocompatible Materials; D001861:Bone Regeneration; D018786:Bone Substitutes; D002516:Ceramics; D015921:Dental Implants; D003764:Dental Materials; D017886:Durapatite; D015726:Giant Cells / Q000648:ultrastructure; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D020007:Implants, Experimental; D008297:Male; D008422:Materials Testing / Q000379:methods*; D008964:Molar, Third / Q000601:surgery; D016062:Porosity; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014081:Tooth Extraction; D014945:Wound Healing; D055114:X-Ray Microtomography / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D015921:Dental Implants; D003764:Dental Materials; D017886:Durapatite