title: Adsorptive property of Cu(2+)-loaded montmorillonite clays for Escherichia coli K88 in vitro.
abstract: The adsorption properties of Cu(2+)-loaded montmorillonite clays (MMT-Cu) for Escherichia coli K88 as a function of time, bacteria concentrations, pH, ionic strength and temperature were investigated. The results showed that the bacteria adsorption onto MMT-Cu surface reached equilibrium after 90 min. The percentages of E. coli K88 adsorbed onto the surfaces of MMT-Cu and montmorillonite clays (MMT) at equilibrium were 88.9% and 56.5%, respectively. Scanning electron microscopy revealed that a lot of E. coli K88 adhered to the surface of MMT-Cu. The zeta potential of MMT-Cu was relatively high as compared to that of MMT. The adsorptive ability of MMT-Cu for E. coli K88 was higher than that of MMT (P < 0.05). Moreover, pH, ionic strength and temperature produced a strong influence on the extent of E. coli K88 adsorption to surface of MMT-Cu and MMT. The mechanism of adsorption of E. coli onto MMT-Cu may involve electrostatic attraction and physiochemical properties of bacterial cell walls and minerals surfaces.
authors.affiliation: Beijing Vocational College of Agriculture, Beijing 102442, China. sxgtong@163.com
authors.forename: Tong
authors.initials: T
authors.lastname: Guo
authors.forename: Shoujun
authors.initials: S
authors.lastname: Cao
authors.forename: Rui
authors.initials: R
authors.lastname: Su
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Li
authors.forename: Ping
authors.initials: P
authors.lastname: Hu
authors.forename: Zirong
authors.initials: Z
authors.lastname: Xu
mesh_terms: D000327:Adsorption; D001546:Bentonite / Q000737:chemistry*; D019327:Copper Sulfate / Q000737:chemistry*; D054307:Enterotoxigenic Escherichia coli / Q000302:isolation & purification*; D008855:Microscopy, Electron, Scanning
chemical_list: D001546:Bentonite; D019327:Copper Sulfate