title: Phytochemical analysis and antioxidant capacity of BM-21, a bioactive extract rich in polyphenolic metabolites from the sea Grass Thalassia testudinum.
abstract: The aqueous ethanol extract of Thalassia testudinum leaves (BM-21) is now being developed in Cuba as an herbal medicine due to its promising pharmacological properties. Although some interesting biological activities of BM-21 have already been reported, its chemical composition remains mostly unknown. Thus, we now describe the qualitative and quantitative analyzes of BM-21 using standard phytochemical screening techniques, including colorimetric quantification, TLC and HPLC analyses. Phytochemical investigation of BM-21 resulted in the isolation and identification of a new phenolic sulfate ester (1), along with ten previously described phenolic derivatives (2-11), seven of which have never been previously reported from the genus Thalassia. The structures of these compounds were established by analysis of their spectroscopic (1D and 2D NMR) and spectrometric (HRMS) data, as well as by comparison of these with those reported in the literature. Furthermore, BM-21 was found to exhibit strong antioxidant activity in four different free radical scavenging assays (HO*, RO2*, O2-* and DPPH*). Consequently, this is the first study which highlights the phytochemical composition of BM-21 and demonstrates that this product is a rich source of natural antioxidants with potential applications in pharmaceutical, cosmetic and food industries.
authors.affiliation: Université de Nice-Sophia Antipolis, Laboratoire de Chimie des Molécules Bioactives et des Arômes, UMR 6001 CNRS, Institut de Chimie de Nice, Faculté des Science, Parc Valrose, 06108 Nice Cedex 02, France. erikluis18@gmail.com
authors.forename: Erik L
authors.initials: EL
authors.lastname: Regalado
authors.forename: Roberto
authors.initials: R
authors.lastname: Menendez
authors.forename: Olga
authors.initials: O
authors.lastname: Valdés
authors.forename: Ruth A
authors.initials: RA
authors.lastname: Morales
authors.forename: Abilio
authors.initials: A
authors.lastname: Laguna
authors.forename: Olivier P
authors.initials: OP
authors.lastname: Thomas
authors.forename: Yasnay
authors.initials: Y
authors.lastname: Hernandez
authors.forename: Clara
authors.initials: C
authors.lastname: Nogueiras
authors.forename: Anake
authors.initials: A
authors.lastname: Kijjoa
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D031321:Hydrocharitaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D059808:Polyphenols / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D059808:Polyphenols