title: Subpopulation composition and activation of T lymphocytes during coculturing with mesenchymal stromal cells in medium with different O(2) content.
abstract: The concentration of O(2) during coculturing practically did not affect the subpopulation composition of T lymphocytes (CD3(+)/CD4(+), CD3(+)/CD8(+), CD3(+)/CD16(+)/CD56(+) T cells) under conditions of PHA-induced activation. Coculturing with mesenchymal stromal cells (MSC) led to a significant decrease in the ratio of lymphocytes carrying activation markers (CD3(+)/CD25(+) and CD3(+)/HLA-DR(+)) and increase in the number of CD3(+)/CD16(+)/CD56(+) T cells. The percent of activated HLA-DR(+) T cells in a heterotypic culture with MSC at 5% O(2) was much lower than that observed under normal conditions of culturing (20% O(2)). Our results suggest that antigen presentation by T lymphocytes due to HLA-DR expression can be reduced in the target tissues at low concentration of O(2), while the interaction between allogeneic MSC probably contributes to more significant inhibition of the immune response.
authors.affiliation: Institute of Medical and Biological Problems, Russian Academy of Sciences, Moscow, Russia. buravkova@imbp.ru
authors.forename: L B
authors.initials: LB
authors.lastname: Buravkova
authors.forename: O G
authors.initials: OG
authors.lastname: Grigorieva
authors.forename: E R
authors.initials: ER
authors.lastname: Andreeva
authors.forename: I V
authors.initials: IV
authors.lastname: Andrianova
authors.forename: Yu V
authors.initials: YV
authors.lastname: Rylova
mesh_terms: D017951:Antigen Presentation; D064987:Cell- and Tissue-Based Therapy / Q000379:methods; D018920:Coculture Techniques; D003470:Culture Media; D006684:HLA-DR Antigens / Q000096:biosynthesis / Q000276:immunology; D006801:Humans; D008213:Lymphocyte Activation; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D010100:Oxygen / Q000032:analysis; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology* / Q000378:metabolism
chemical_list: D003470:Culture Media; D006684:HLA-DR Antigens; D010100:Oxygen