title: An unusual spliced variant of DELLA protein, a negative regulator of gibberellin signaling, in lettuce.
abstract: DELLA proteins are negative regulators of the signaling of gibberellin (GA), a phytohormone regulating plant growth. DELLA degradation is triggered by its interaction with GID1, a soluble GA receptor, in the presence of bioactive GA. We isolated cDNA from a spliced variant of LsDELLA1 mRNA in lettuce, and named it LsDELLA1sv. It was deduced that LsDELLA1sv encodes truncated LsDELLA1, which has DELLA and VHYNP motifs at the N terminus but lacks part of the C-terminal GRAS domain. The recombinant LsDELLA1sv protein interacted with both Arabidopsis GID1 and lettuce GID1s in the presence of GA. A yeast two-hybrid assay suggested that LsDELLA1sv interacted with LsDELLA1. The ratio of LsDELLA1sv to LsDELLA1 transcripts was higher in flower samples at the late reproductive stage and seed samples (dry seeds and imbibed seeds) than in the other organ samples examined. This study suggests that LsDELLA1sv is a possible modulator of GA signaling in lettuce.
authors.affiliation: Department of Bioresource Engineering, Yamagata University, Tsutuoka, Yamagata, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Sawada
authors.forename: Asami
authors.initials: A
authors.lastname: Umetsu
authors.forename: Yuki
authors.initials: Y
authors.lastname: Komatsu
authors.forename: Jun
authors.initials: J
authors.lastname: Kitamura
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Suzuki
authors.forename: Tadao
authors.initials: T
authors.lastname: Asami
authors.forename: Machiko
authors.initials: M
authors.lastname: Fukuda
authors.forename: Ichiro
authors.initials: I
authors.lastname: Honda
authors.forename: Wataru
authors.initials: W
authors.lastname: Mitsuhashi
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Nakajima
authors.forename: Tomonobu
authors.initials: T
authors.lastname: Toyomasu
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D005875:Gibberellins / Q000378:metabolism*; D018545:Lactuca / Q000166:cytology* / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D018076:DNA, Complementary; D005875:Gibberellins; D010940:Plant Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger; D011956:Receptors, Cell Surface