title: [miR-124-1 promotes neural differentiation of rat bone marrow mesenchymal stem cells].
abstract: OBJECTIVE
  To study the effects of miR-124-1 on neuronal differentiation of rat bone marrow mesenchymal stem cells (MSCs).
  
  
  METHODS
  MSCs cells were assigned into three groups: control (uninfected and untransfected), miR-124-1+ (infected with miR-124-1), and miR-124-1- (transfected with Anti-rno-miR-124* Inhibitor). MSCs were induced by β-mercaptoethanol (β-ME) to differentiate into neurons. The fluorescence expressed by infected MSCs was observed under an inverted fluorescence microscope. MTT method was used to measure cell survival rate after transfection or infection. Immunocytochemistry, RT-PCR and Western blot methods were used to detect the expression of β3 tubulin, MAP-2 and GFAP 6 days after β-ME induction.
  
  
  RESULTS
  The expression of miR-124-1 in the miR-124-1+ group was significantly higher 2 days after infection of lentivirus vector compared with the control group (P<0.01). In the miR-124-1- group, the cell survival rate and the miR-124-1 expression level decreased significantly 24 hrs after transfection of anti-rno-miR-124* inhibitor (P<0.01). After 6 days of β-ME induction, the protein and mRNA expression levels of β3 tubulin and MAP-2 in the miR-124-1+ group were much higher than the other two groups (P<0.01); while the expression levels of β3 tubulin and MAP-2 in the miR-124-1-group were lower than the control group (P<0.01). The expression of GFAP in the three groups was weak (<1%).
  
  
  CONCLUSIONS
  miR-124 might promote neuronal differentiation of rat MSCs.
authors.affiliation: Department of Radiology, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Kui-Sheng
authors.initials: KS
authors.lastname: Chen
authors.forename: Jian-Bo
authors.initials: JB
authors.lastname: Gao
authors.forename: Rui
authors.initials: R
authors.lastname: Han
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Lu
authors.forename: Tao
authors.initials: T
authors.lastname: Peng
authors.forename: Yan-Jie
authors.initials: YJ
authors.lastname: Jia
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D035683:MicroRNAs / Q000502:physiology*; D008869:Microtubule-Associated Proteins / Q000032:analysis; D009474:Neurons / Q000166:cytology*; D051381:Rats; D017208:Rats, Wistar; D014404:Tubulin / Q000032:analysis
chemical_list: D005904:Glial Fibrillary Acidic Protein; C489787:MAP2 protein, rat; C548144:MIRN124 microRNA, rat; D035683:MicroRNAs; D008869:Microtubule-Associated Proteins; C488416:Tubb3 protein, rat; D014404:Tubulin