title: Structural polymorphism of human telomere G-quadruplex induced by a pyridyl carboxamide molecule.
abstract: In this work, we described a kinetically slow (hour-scale) but thermodynamically favored G-quadruplex conversion induced by a pyridyl carboxamide molecule. This slow transition was observed through CD spectra and gels, and its final stable parallel conformation was identified by 2-aminopurine experiments. Kinetic experiments indicated that this slow process was a first-order reaction, implying it was a unimolecular conversion. Quite distinctly from other reported ligand-driven G-quadruplex conformation alteration, this slow conversion reveals a novel insight into G-quadruplex polymorphism, in accordance with the behavior of human telomere G-quadruplex in a molecular crowding environment in K(+) solution, further enriching the known structural polymorphism of human telomere DNA and providing new consideration for drug design based on G-quadruplexes.
authors.affiliation: College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, State Key Laboratory of Virology, Wuhan University, Hubei, Wuhan 430072, PR China.
authors.forename: Liang
authors.initials: L
authors.lastname: Xu
authors.forename: Zhouxin
authors.initials: Z
authors.lastname: Xu
authors.forename: Ye
authors.initials: Y
authors.lastname: Shang
authors.forename: Shuo
authors.initials: S
authors.lastname: Feng
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhou
mesh_terms: D001483:Base Sequence; D002942:Circular Dichroism; D054856:G-Quadruplexes / Q000187:drug effects; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D015394:Molecular Structure; D011725:Pyridines / Q000737:chemistry* / Q000494:pharmacology; D016615:Telomere / Q000737:chemistry* / Q000187:drug effects; D013816:Thermodynamics
chemical_list: C453600:N-(1,2-bis(2-pyridyl)ethyl)pyridine-2-carboxamide; D011725:Pyridines