title: Expression of complement components and regulators by different subtypes of bone marrow-derived macrophages.
abstract: Under inflammatory conditions, macrophages can differentiate into different functional subtypes. We show that bone marrow-derived macrophages constitutively express different levels of various complement-related genes. The relative expression levels are C1qb > Crry > CFH > C3 > C1r > CFB > DAF1 > CD59a > C2 > C1INH > C1s > C4. Upon activation, the expression of C1r, C1s, C3, C2, CFB, and C1INH was up-regulated, and CFH, CD59a, and DAF1, down-regulated in M1 (induced by interferon-γ + lipopolysaccharides (LPS)) and M2b (induced by immune complex + LPS) macrophages. The expression of C4 and CFH was slightly up-regulated in interleukin (IL)-10-induced M2c macrophages. Complement gene expression in IL-4-induced M2a macrophages was weakly down-regulated as compared to resting M0 macrophages. Higher levels of C3, C1INH, and CFB but lower levels of CFH expression in M1 and M2b macrophage suggests that they may be involved in the alternative pathway of complement activation during inflammation.
authors.affiliation: Centre for Vision and Vascular Science, School of Medicine, Dentistry and Biomedical Sciences, Queen's University Belfast, Grosvenor Road, BT12 6BA, Belfast, UK.
authors.forename: Chang
authors.initials: C
authors.lastname: Luo
authors.forename: Mei
authors.initials: M
authors.lastname: Chen
authors.forename: Angelina
authors.initials: A
authors.lastname: Madden
authors.forename: Heping
authors.initials: H
authors.lastname: Xu
mesh_terms: D000818:Animals; D000936:Antigen-Antibody Complex / Q000378:metabolism; D002478:Cells, Cultured; D003167:Complement Activation; D003165:Complement System Proteins / Q000096:biosynthesis*; D016207:Cytokines / Q000378:metabolism; D015536:Down-Regulation; D015870:Gene Expression; D007249:Inflammation / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D008264:Macrophages / Q000145:classification / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D015854:Up-Regulation
chemical_list: D000936:Antigen-Antibody Complex; D016207:Cytokines; D008070:Lipopolysaccharides; D003165:Complement System Proteins