title: Crystallization and preliminary X-ray analysis of the subclass B3 metallo-β-lactamase SMB-1 that confers carbapenem resistance.
abstract: The carbapenem-hydrolyzing subclass B3 metallo-β-lactamase SMB-1 was expressed in Escherichia coli and purified. Diffraction data were collected from two types of SMB-1 crystals that were obtained under different conditions. One crystal (SMB-1a) belonged to the trigonal space group P3(1) with unit-cell parameters a = b = 67.83, c = 48.67 Å, while the other crystal (SMB-1b) also belonged to space group P3(1) but with unit-cell parameters a = b = 67.25, c = 46.83 Å. Both crystals contained one molecule per asymmetric unit. Initial phases were determined by molecular replacement; further refinement and model building are in progress.
authors.affiliation: Department of Bacteriology II, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashi-Murayama, Tokyo 208-0011, Japan. wachino@nih.go.jp
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Wachino
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Yamaguchi
authors.forename: Shigetarou
authors.initials: S
authors.lastname: Mori
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Yamagata
authors.forename: Yoshichika
authors.initials: Y
authors.lastname: Arakawa
authors.forename: Keigo
authors.initials: K
authors.lastname: Shibayama
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D004754:Enterobacter / Q000201:enzymology*; D001618:beta-Lactamases / Q000737:chemistry*
chemical_list: D001618:beta-Lactamases