title: Characterization of a gene encoding prohibitin in silkworm, Bombyx mori.
abstract: BACKGROUND
  Prohibitin (PHB) is an evolutionarily conserved multifunctional protein with ubiquitous expression. However, its molecular roles are largely unknown.
  
  
  METHODS
  To better understand the function of prohibitin protein in silkworm (BmPHB), its coding sequence was isolated from a cDNA library of silkworm pupae. An His-tagged BmPHB fusion protein was expressed in Escherichia coli Rosetta (DE3) and purified with affinity and reversed-phase chromatography. Purified rBmPHB was used to generate anti-BmPHB polyclonal antibody. The subcellular localization of BmPHB was analysed by immunohistochemistry.
  
  
  RESULTS
  BmPHB gene has an ORF of 825 bp, encoding a predicted peptide with 274 amino acid residues. Immunostaining indicate that prohibitin is expressed in nucleus and predominately in cytoplasm. Western blot analyses indicated that, in the fifth instar larva, BmPHB was expressed descendingly in gonad, malpighian tubule, trachea, fatty body, intestine, and head. However, no expression was detected in larva's silk gland and epidermis. In addition, BmPHB was expressed in the nascent egg, larva and pupa, but not in the moth.
  
  
  CONCLUSIONS
  The expression of BmPHB gene presents differential characteristic in different stage and tissues. It may play important roles in the development of silkworm.
  
  
  CONCLUSIONS
  Studies on prohibitin have been still restricted to a few specific insects and insect cell lines such as Drosophila, Acyrthosiphon pisum and mosquito cell lines, not yet in silkworm. This is a first characterization of prohibitin in silkworm, B. mori.
authors.affiliation: Institute of Biochemistry, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, 310018, China.
authors.forename: Zhengbing
authors.initials: Z
authors.lastname: Lv
authors.forename: Xufang
authors.initials: X
authors.lastname: Zhang
authors.forename: Lili
authors.initials: L
authors.lastname: Liu
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.forename: Zuoming
authors.initials: Z
authors.lastname: Nie
authors.forename: Qing
authors.initials: Q
authors.lastname: Sheng
authors.forename: Wenping
authors.initials: W
authors.lastname: Zhang
authors.forename: Caiying
authors.initials: C
authors.lastname: Jiang
authors.forename: Wei
authors.initials: W
authors.lastname: Yu
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.forename: Xiangfu
authors.initials: X
authors.lastname: Wu
authors.forename: Shijun
authors.initials: S
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Yaozhou
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000235:genetics / Q000302:isolation & purification; D000917:Antibody Formation / Q000235:genetics; D001483:Base Sequence; D012831:Bombyx / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular / Q000379:methods; D019295:Computational Biology / Q000379:methods; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D007150:Immunohistochemistry / Q000379:methods; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D000091402:Prohibitins; D011679:Pupa / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D000906:Antibodies; D019476:Insect Proteins; D000091402:Prohibitins; D011994:Recombinant Proteins; D012097:Repressor Proteins