title: The use of rpoB sequence analysis in the differentiation of Mycobacterium abscessus and Mycobacterium chelonae: a critical judgement in cystic fibrosis?
abstract: Individuals suffering from fibrocystic disease may acquire non-tuberculous mycobacteria as colonizing or infecting organisms. Mycobacterium abscessus is of particular concern because it may be very difficult to eradicate and may mitigate against lung transplantation. However, this species may be difficult to reliably differentiate from the closely related M. chelonae. We have developed a rapid, low-cost, short sequence-based technique to confirm species identity by analysis of a segment of the RNA Polymerase B (rpoB) gene.
authors.affiliation: Applied and Functional Genomics Unit, HPA Microbiology Services, Centre for Infections, London, UK.
authors.forename: C
authors.initials: C
authors.lastname: Arnold
authors.forename: A
authors.initials: A
authors.lastname: Barrett
authors.forename: L
authors.initials: L
authors.lastname: Cross
authors.forename: J G
authors.initials: JG
authors.lastname: Magee
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D003550:Cystic Fibrosis / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D009165:Mycobacterium Infections, Nontuberculous / Q000382:microbiology; D016926:Mycobacterium chelonae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D009170:Nontuberculous Mycobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012319:RNA Polymerase II / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods*; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D012319:RNA Polymerase II