title: Differential subcellular localization of DNA topoisomerase-1 isoforms and their roles during Caenorhabditis elegans development.
abstract: DNA topoisomerase-1 (TOP-1) resolves the topological problems associated with DNA replication, transcription and recombination by introducing temporary single-strand breaks in the DNA. Caenorhabditis elegans TOP-1 has two isoforms, TOP-1α and TOP-1β. TOP-1β is broadly localized to the nuclei of many cells at all developmental stages and concentrated in nucleoli in embryo gut and oogenic cells. However, TOP-1α is specifically localized to centrosomes, neuronal cells, excretory cells and chromosomes of germ cells in embryonic and larval stages. Reporter gene analysis also shows that top-1 transcription is highly activated in several sensory neurons, speculating the possible role of TOP-1α in neuronal development. From RNA interference (RNAi) experiments, we demonstrated that C. elegans TOP-1 is required for chromosomal segregation, germline proliferation and gonadal migration, which are all correlated with the expression and activity of TOP-1. Therefore, our findings may provide an insight into a new role of TOP-1 in development of multicellular organisms.
authors.affiliation: Division of Hematology/Oncology, Brody School of Medicine, East Carolina University, Greenville, NC 27834, USA.
authors.forename: Dong Seok
authors.initials: DS
authors.lastname: Cha
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Hollis
authors.forename: Udaya Sree
authors.initials: US
authors.lastname: Datla
authors.forename: Sejin
authors.initials: S
authors.lastname: Lee
authors.forename: Jinsun
authors.initials: J
authors.lastname: Ryu
authors.forename: Hey Rim
authors.initials: HR
authors.lastname: Jung
authors.forename: Eunsuk
authors.initials: E
authors.lastname: Kim
authors.forename: Kyuhyung
authors.initials: K
authors.lastname: Kim
authors.forename: Myeongwoo
authors.initials: M
authors.lastname: Lee
authors.forename: Chris
authors.initials: C
authors.lastname: Li
authors.forename: Myon-Hee
authors.initials: MH
authors.lastname: Lee
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D017173:Caenorhabditis elegans / Q000737:chemistry / Q000196:embryology* / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000378:metabolism; D020090:Chromosome Segregation; D004264:DNA Topoisomerases, Type I / Q000032:analysis / Q000378:metabolism*; D006066:Gonads / Q000196:embryology / Q000378:metabolism; D007527:Isoenzymes / Q000032:analysis / Q000378:metabolism
chemical_list: D029742:Caenorhabditis elegans Proteins; D007527:Isoenzymes; D004264:DNA Topoisomerases, Type I