title: A novel WD-repeat protein, WDR26, inhibits apoptosis of cardiomyocytes induced by oxidative stress.
abstract: WD40 repeat proteins have a variety of functions, such as signal transduction, transcription regulation, cell cycle control, autophagy and apoptosis. WDR26 is a novel protein of WD40 repeat proteins and up-regulated during myocardial cells ischemic preconditioning (IPC) but its role in myocardial cell apoptosis induced by oxidative stress and its subcellular localisation are not clear. So we investigated the subcellular localisation of WDR26 and WDR26 expression in rat myocardial ischaemia-reperfusion injury model and H9c2 cells stimulated by H(2)O(2). The results showed that WDR26 can be located at mitochondria and induced by ischaemia-reperfusion injury and H(2)O(2). Then we examined the effects induced by H(2)O(2) in H9c2 cells WDR26 expression. The results showed that WDR26 expression can inhibit apoptosis induced by H(2)O(2). Further, we demonstrated that WDR26 inhibit cytochrome c release from mitochondria. These founding indicate that WDR26 protects myocardial cells against oxidative stress.
authors.affiliation: Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha, Hunan, People's Republic of China.
authors.forename: Yansheng
authors.initials: Y
authors.lastname: Feng
authors.forename: Chi
authors.initials: C
authors.lastname: Zhang
authors.forename: Qi
authors.initials: Q
authors.lastname: Luo
authors.forename: Xing
authors.initials: X
authors.lastname: Wei
authors.forename: Bimei
authors.initials: B
authors.lastname: Jiang
authors.forename: Honglin
authors.initials: H
authors.lastname: Zhu
authors.forename: Lingli
authors.initials: L
authors.lastname: Zhang
authors.forename: Lei
authors.initials: L
authors.lastname: Jiang
authors.forename: Meidong
authors.initials: M
authors.lastname: Liu
authors.forename: Xianzhong
authors.initials: X
authors.lastname: Xiao
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D002470:Cell Survival / Q000502:physiology; D004195:Disease Models, Animal; D047908:Intracellular Signaling Peptides and Proteins / Q000096:biosynthesis / Q000378:metabolism*; D018613:Microscopy, Confocal; D015428:Myocardial Reperfusion Injury / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction
chemical_list: D047908:Intracellular Signaling Peptides and Proteins