title: The activation of CD99 inhibits cell-extracellular matrix adhesion by suppressing β(1) integrin affinity.
abstract: CD99 is known to be involved in the regulation of cell-cell adhesion. However, it remains unclear whether CD99 controls cell-extracellular matrix adhesion. In this study, the effects of CD99 activation on cell-extracellular matrix adhesion were investigated. It was found that engagement of CD99 with the stimulating antibody YG32 downregulated the adhesion of MCF-7 cells to fibronectin, laminin and collagen IV in a dose-dependent manner. The CD99 effect on cell-ECM adhesion was inhibited by overexpression of the dominant negative form of CD99 or CD99 siRNA transfection. Treatment of cells with Mn(2+) or by β(1) integrin-stimulating antibody restored the inhibitory effect of CD99 on cell-ECM adhesion. Cross-linking CD99 inactivated β(1) integrin through conformational change. CD99 activation caused dephosphorylation at Tyr-397 in FAK, which was restored by the β(1) stimulating antibody. Taken together, these results provide the first evidence that CD99 inhibits cell-extracellular matrix adhesion by suppressing β(1) integrin affinity.   [BMB reports 2012; 45(3): 159-164].
authors.affiliation: Departments of Anatomy and Cell Biology, School of Medicine, College of Natural Sciences, Kangwon National University, Chunchon, Korea.
authors.forename: Kyoung-Jin
authors.initials: KJ
authors.lastname: Lee
authors.forename: Sun-Hee
authors.initials: SH
authors.lastname: Lee
authors.forename: Birendra Kumar
authors.initials: BK
authors.lastname: Yadav
authors.forename: Hyun-Mi
authors.initials: HM
authors.lastname: Ju
authors.forename: Min-Seo
authors.initials: MS
authors.lastname: Kim
authors.forename: Jeong Hyun
authors.initials: JH
authors.lastname: Park
authors.forename: Dooil
authors.initials: D
authors.lastname: Jeoung
authors.forename: Hansoo
authors.initials: H
authors.lastname: Lee
authors.forename: Jang-Hee
authors.initials: JH
authors.lastname: Hahn
mesh_terms: D000071443:12E7 Antigen; D015703:Antigens, CD / Q000378:metabolism*; D002448:Cell Adhesion / Q000187:drug effects; D015815:Cell Adhesion Molecules / Q000819:agonists* / Q000378:metabolism*; D005109:Extracellular Matrix / Q000187:drug effects* / Q000378:metabolism*; D006801:Humans; D019012:Integrin beta1 / Q000378:metabolism*; D061986:MCF-7 Cells; D014407:Tumor Cells, Cultured
chemical_list: D000071443:12E7 Antigen; D015703:Antigens, CD; C061828:CD99 protein, human; D015815:Cell Adhesion Molecules; D019012:Integrin beta1