title: Protective effect of isoorientin-2″-O-α-L-arabinopyranosyl isolated from Gypsophila elegans on alcohol induced hepatic fibrosis in rats.
abstract: Alcohol abuse is one of the major causes of liver fibrosis, which shows a sharply increasing trend worldwide, yet effective therapeutic options for advanced alcohol fibrosis are limited. Recently we investigated the effect of anti-fibrosis by isoorientin-2″-O-α-L-arabinopyranosyl (IOA) isolated from Gypsophila elegans. During the experiment, the model group received alcohol only, and treatment groups received the corresponding drugs plus alcohol respectively, while the normal control group received an equal volume of saline. Analysis at the end of 24-week experiments showed that IOA could significantly improve the liver function, as indicated by decreasing levels of alanine transaminase, aspartate transaminase, alkaline phosphatase, γ-glutamyltransferase, interleukin-6 and tumor necrosis factor-α. Moreover, IOA could effectively inhibit collagen deposition and reduce the pathological tissue damage. Research on mechanism showed that IOA was able to markedly reduce lipid peroxidation, recruit the anti-oxidative defense system, and induce HSC apoptosis by down-regulating bcl-2 mRNA, as well as inhibit the expression of α-smooth muscle actin and transforming growth factor β1 proteins. In short, our results showed that IOA is effective in attenuating hepatic injury and fibrosis in the alcohol-induced rat model, which should be developed as a new drug to treat liver fibrosis and even cirrhosis.
authors.affiliation: The First Affiliated Hospital Of Guangxi Traditional Chinese Medicine University, Nanning 530023, China.
authors.forename: Quan Fang
authors.initials: QF
authors.lastname: Huang
authors.forename: Shi Jun
authors.initials: SJ
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Zheng
authors.forename: Ming
authors.initials: M
authors.lastname: Liao
authors.forename: Min
authors.initials: M
authors.lastname: He
authors.forename: RenBin
authors.initials: R
authors.lastname: Huang
authors.forename: Lang
authors.initials: L
authors.lastname: Zhuo
authors.forename: Xing
authors.initials: X
authors.lastname: Lin
mesh_terms: D000199:Actins / Q000378:metabolism; D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D001219:Aspartate Aminotransferases / Q000097:blood; D015153:Blotting, Western; D029748:Caryophyllaceae / Q000737:chemistry*; D003078:Colchicine / Q000494:pharmacology; D004187:Disaccharides / Q000737:chemistry / Q000494:pharmacology*; D047309:Flavones / Q000737:chemistry / Q000494:pharmacology*; D006519:Hepatitis, Alcoholic / Q000473:pathology* / Q000517:prevention & control*; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000201:enzymology / Q000473:pathology*; D008103:Liver Cirrhosis / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
chemical_list: D000199:Actins; D000975:Antioxidants; D004187:Disaccharides; D047309:Flavones; D019253:Proto-Oncogene Proteins c-bcl-2; D053773:Transforming Growth Factor beta1; C575436:isoorientin-2''-O-arabinopyranosyl; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; D003078:Colchicine