title: Survival of the aerobic denitrifier Pseudomonas stutzeri strain TR2 during co-culture with activated sludge under denitrifying conditions.
abstract: The aerobic denitrifier Pseudomonas stutzeri TR2 (strain TR2) has the potential to reduce nitrous oxide emissions during the wastewater treatment process. In this application, it is important to find the best competitive survival conditions for strain TR2 in complex ecosystems. To that end, we examined co-cultures of strain TR2 with activated sludge via five passage cultures in a medium derived from treated piggery wastewater that contained a high concentration of ammonium. The results are as follows: (i) The medium supported the proliferation of strain TR2 (P. stutzeri strains) under denitrifying conditions. (ii) Nitrite was a better denitrification substrate than nitrate for TR2 survival. (iii) Strain TR2 also demonstrated strong survival even under aerobic conditions. This suggests that strain TR2 is effectively augmented to the wastewater treatment process, aiding in ammonium-nitrogen removal and reducing nitrous oxide production with a partial nitrification technique in which nitrite accumulates.
authors.affiliation: Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
authors.forename: Morio
authors.initials: M
authors.lastname: Miyahara
authors.forename: Sang-Wan
authors.initials: SW
authors.lastname: Kim
authors.forename: Shengmin
authors.initials: S
authors.lastname: Zhou
authors.forename: Shinya
authors.initials: S
authors.lastname: Fushinobu
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yamada
authors.forename: Wakako
authors.initials: W
authors.lastname: Ikeda-Ohtsubo
authors.forename: Akira
authors.initials: A
authors.lastname: Watanabe
authors.forename: Keisuke
authors.initials: K
authors.lastname: Miyauchi
authors.forename: Ginro
authors.initials: G
authors.lastname: Endo
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Wakagi
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Shoun
mesh_terms: D000332:Aerobiosis; D001673:Biodegradation, Environmental; D018920:Coculture Techniques; D058440:Denitrification; D050296:Microbial Viability; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D044223:Pseudomonas stutzeri / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D012722:Sewage / Q000382:microbiology*
chemical_list: D012722:Sewage