title: Sorting nexins 1 and 2a locate mainly to the TGN.
abstract: The subcellular localization of the sorting nexins (SNXs) in higher plants is a matter of controversy. Previous confocal laser scanning microscopy (CLSM studies on root cells from a transgenic Arabidopsis line expressing SNX1-GFP have suggested that this SNX is present on an endosome having characteristics of both the trans-Golgi network (TGN) and the multivesicular body (MVB). In contrast, SNX2a locates exclusively to the TGN when transiently expressed in tobacco mesophyll protoplasts. By performing immunogold electron microscopy on cryofixed Arabidopsis roots, we have tried to clarify the situation. Both SNX1-GFP and endogenous SNX2a locate principally to the TGN. Labeling of MVBs could not be confirmed with any certainty.
authors.affiliation: Microscopy, Centre for Plant Molecular Biology, University of Tübingen, 72076 Tübingen, Germany.
authors.forename: York-Dieter
authors.initials: YD
authors.lastname: Stierhof
authors.forename: Corrado
authors.initials: C
authors.lastname: Viotti
authors.forename: David
authors.initials: D
authors.lastname: Scheuring
authors.forename: Silke
authors.initials: S
authors.lastname: Sturm
authors.forename: David G
authors.initials: DG
authors.lastname: Robinson
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D029681:Arabidopsis Proteins / Q000378:metabolism*; D011992:Endosomes / Q000378:metabolism / Q000648:ultrastructure; D007150:Immunohistochemistry; D018613:Microscopy, Confocal; D057146:Multivesicular Bodies; D030821:Plants, Genetically Modified; D021381:Protein Transport; D011523:Protoplasts / Q000378:metabolism; D058305:Sorting Nexins / Q000378:metabolism*; D021601:trans-Golgi Network / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D029681:Arabidopsis Proteins; D058305:Sorting Nexins