title: Influence of three laser wavelengths on human fibroblasts cell culture.
abstract: Although experimental studies in vitro and vivo have been numerous, the effect of laser wavelength irradiation on human fibroblast cell culture is poorly understood. This emphasizes the need of additional cellular and molecular research into laser influence with low energy and power. The aim of this study was to assess the influence of three different laser wavelengths on the human skin fibroblasts cell culture. We wanted to evaluate if near infrared lasers had any influence in healing of wounds by stimulating mitochondrial activity of fibroblasts. The cells were irradiated using 830-, 980- and 2,940-nm laser wavelengths. The irradiated cells were incubated and their mitochondrial activity was assessed by the MTT assay at 24, 48 and 72 h. Simultaneously, an apoptosis assay was assessed on the irradiated fibroblasts. It can be concluded that laser light of the near-infrared region (830 and 980 nm) influences fibroblasts mitochondrial activity compared to the 2,940-nm wavelength which produces apoptosis.
authors.affiliation: Department of Implantology and Maxillofacial Surgery, University of Medicine and Pharmacy Iuliu Hatieganu, No. 37, Cardinal Iuliu Hossu Street, 400029, Cluj-Napoca, Romania. bbcrisan@yahoo.com
authors.forename: Bogdan
authors.initials: B
authors.lastname: Crisan
authors.forename: Olga
authors.initials: O
authors.lastname: Soritau
authors.forename: Mihaela
authors.initials: M
authors.lastname: Baciut
authors.forename: Radu
authors.initials: R
authors.lastname: Campian
authors.forename: Liana
authors.initials: L
authors.lastname: Crisan
authors.forename: Grigore
authors.initials: G
authors.lastname: Baciut
mesh_terms: D017209:Apoptosis / Q000528:radiation effects; D002478:Cells, Cultured; D004307:Dose-Response Relationship, Radiation; D005347:Fibroblasts / Q000528:radiation effects*; D006801:Humans; D007259:Infrared Rays; D053685:Laser Therapy / Q000379:methods*; D008928:Mitochondria / Q000378:metabolism / Q000528:radiation effects; D014945:Wound Healing / Q000528:radiation effects