title: Molecular structure of isolated MvspI, a variable surface protein of the fish pathogen Mycoplasma mobile.
abstract: Mycoplasma mobile is a parasitic bacterium that causes necrosis in the gills of freshwater fishes. This study examines the molecular structure of its variable surface protein, MvspI, whose open reading frame encodes 2,002 amino acids. MvspI was isolated from mycoplasma cells by a biochemical procedure to 92% homogeneity. Gel filtration and analytical ultracentrifugation suggested that this protein is a cylinder-shaped monomer with axes of 66 and 2.7 nm. Rotary shadowing transmission electron microscopy of MvspI showed that the molecule is composed of two rods 30 and 45 nm long; the latter rod occasionally features a bulge. Immuno-electron microscopy and epitope mapping showed that the bulge end of the molecular image corresponds to the C terminus of the amino acid sequence. Partial digestion by various proteases suggested that the N-terminal part, comprised of 697 amino acids, is flexible. Analysis of the predicted amino acid sequence showed that the molecule features a lipoprotein and 16 repeats of about 90 residues; 15 positions exist between residues 88 and 1479, and the other position is between residues 1725 and 1807. The amino acid sequence of MvspI was mapped onto a molecular image obtained by electron microscopy. The present study is the first to elucidate the molecular shape of a variable surface protein of mycoplasma.
authors.affiliation: Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi-ku, Osaka, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Adan-Kubo
authors.forename: Shu-hei
authors.initials: SH
authors.lastname: Yoshii
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Kono
authors.forename: Makoto
authors.initials: M
authors.lastname: Miyata
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000648:ultrastructure; D008565:Membrane Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D016253:Microscopy, Immunoelectron; D015394:Molecular Structure; D009174:Mycoplasma / Q000737:chemistry*; D013194:Staining and Labeling / Q000379:methods
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins