title: Autonomous murine T-cell progenitor production in the extra-embryonic yolk sac before HSC emergence.
abstract: The extra-embryonic yolk sac (YS) is the first hematopoietic site in the mouse embryo and is thought to generate only primitive erythroid and myeloerythroid progenitor cells before definitive HSC emergence within the embryo on E10.5. Here, we have shown the existence of T cell-restricted progenitors in the E9.5 YS that directly engraft in recipient immunodeficient mice. T-cell progenitors were also produced in vitro from both YS and para-aortic splanchnopleura hemogenic endothelial cells, and these T-cell progenitors repopulated the thymus and differentiated into mature T-cell subsets in vivo on transplantation. Our data confirm that the YS produces T-lineage-restricted progenitors that are available to colonize the thymus and provide new insight into the YS as a definitive hematopoietic site in the mouse embryo.
authors.affiliation: Department of Pediatrics, Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
authors.forename: Momoko
authors.initials: M
authors.lastname: Yoshimoto
authors.forename: Prashanth
authors.initials: P
authors.lastname: Porayette
authors.forename: Nicole L
authors.initials: NL
authors.lastname: Glosson
authors.forename: Simon J
authors.initials: SJ
authors.lastname: Conway
authors.forename: Nadia
authors.initials: N
authors.lastname: Carlesso
authors.forename: Angelo A
authors.initials: AA
authors.lastname: Cardoso
authors.forename: Mark H
authors.initials: MH
authors.lastname: Kaplan
authors.forename: Mervin C
authors.initials: MC
authors.lastname: Yoder
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001011:Aorta / Q000196:embryology; D002454:Cell Differentiation; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000166:cytology / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology / Q000637:transplantation; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology; D015017:Yolk Sac / Q000166:cytology* / Q000276:immunology*