title: Micronucleus assay as a biomarker of genotoxicity in the occupational exposure to agrochemicals in rural workers.
abstract: This paper aims to evaluate the genotoxic effect of agrochemicals in rural workers occupationally exposed by the micronucleus assay in peripheral blood lymphocytes and to promote the development of health and environmental preventive and protective practices. A total of 30 blood samples from 20 individuals occupationally exposed to different agrochemicals and 10 unexposed persons, who formed the reference group, were analyzed. We found statistically significant differences (p < 0.0005, Student's t Test) in the frequency of micronuclei between the two groups (7.20 ± 1.55 and 15.15 ± 5.10 CBMN for reference and exposed groups respectively). The analysis of age showed a positive correlation (Pearson Correlation Test) with the frequency of micronuclei in exposed population (p < 0.05; r(2) = 0.47), in contrast with smoking habits and years of exposure. Micronucleus assay allows an early detection of populations at higher risk of having genetic damage, allowing us to implement strategies of intervention for the purpose of contributing to reduce that risk.
authors.affiliation: Facultad de Ciencias Exactas, Físico-Químicas y Naturales, UNRC, Ruta 36, KM 601, 5800, Río Cuarto, Argentina.
authors.forename: N
authors.initials: N
authors.lastname: Gentile
authors.forename: F
authors.initials: F
authors.lastname: Mañas
authors.forename: B
authors.initials: B
authors.lastname: Bosch
authors.forename: L
authors.initials: L
authors.lastname: Peralta
authors.forename: N
authors.initials: N
authors.lastname: Gorla
authors.forename: D
authors.initials: D
authors.lastname: Aiassa
mesh_terms: D000293:Adolescent; D000328:Adult; D000383:Agriculture; D016573:Agrochemicals / Q000378:metabolism* / Q000633:toxicity; D015415:Biomarkers / Q000097:blood; D004784:Environmental Monitoring; D006801:Humans; D008297:Male; D015162:Micronucleus Tests; D008875:Middle Aged; D009153:Mutagens / Q000378:metabolism* / Q000633:toxicity; D016273:Occupational Exposure / Q000032:analysis / Q000706:statistics & numerical data*; D012424:Rural Population / Q000706:statistics & numerical data*; D055815:Young Adult
chemical_list: D016573:Agrochemicals; D015415:Biomarkers; D009153:Mutagens