title: Association between KLK3 rs2735839 G/A polymorphism and serum PSA levels in Japanese men.
abstract: OBJECTIVE
  To clarify the association of kallikrein-related peptidase 3 (KLK3) rs2735839 G/A polymorphism with serum prostate-specific antigen (PSA) levels in Japanese men.
  
  
  METHODS
  Subjects were participants of the Japan Multi-Institutional Collaborative Cohort (J-MICC) Study who visited the Seirei Preventive Health Care Center in Shizuoka, Japan. Among the 5,040 individuals aged 35-69 years who were enrolled in 2006-2007, serum PSA data were available for 2,323 male subjects without a past history of prostate cancer. The diagnostic criteria for PSA positivity was PSA ≥ 4.0 ng/ml. Genotyping of the KLK3 polymorphism was conducted by the polymerase chain reaction with the confronting two-pair primers (PCR-CTPP) method.
  
  
  RESULTS
  The mean ± SD of PSA levels (mg/dl) were 1.54 ± 1.73 for those with KLK3 rs2735839 G/G genotype, 1.34 ± 1.33 for G/A, and 1.20 ± 1.23 for A/A, which was significantly different (p < 0.0001). The age-adjusted odds ratios of PSA test positivity were 0.62 (95% confidence interval 0.41-0.94) for those with G/A + A/A relative to those with G/G.
  
  
  CONCLUSIONS
  The present study revealed that the KLK3 rs2735839 G allele was significantly associated with higher serum PSA levels also in Japanese.
authors.affiliation: Seirei Preventive Health Care Center, Hamamatsu, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Syunsuke
authors.initials: S
authors.lastname: Nobata
authors.forename: Asahi
authors.initials: A
authors.lastname: Hishida
authors.forename: Mariko
authors.initials: M
authors.lastname: Naito
authors.forename: Yatami
authors.initials: Y
authors.lastname: Asai
authors.forename: Atsuyoshi
authors.initials: A
authors.lastname: Mori
authors.forename: Mayumi
authors.initials: M
authors.lastname: Kuwabara
authors.forename: Shiro
authors.initials: S
authors.lastname: Katase
authors.forename: Rieko
authors.initials: R
authors.lastname: Okada
authors.forename: Emi
authors.initials: E
authors.lastname: Morita
authors.forename: Sayo
authors.initials: S
authors.lastname: Kawai
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Hamajima
authors.forename: Kenji
authors.initials: K
authors.lastname: Wakai
mesh_terms: D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D044466:Asian People / Q000235:genetics*; D016009:Chi-Square Distribution; D003430:Cross-Sectional Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D007564:Japan; D007610:Kallikreins / Q000097:blood* / Q000235:genetics*; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D010641:Phenotype; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D017430:Prostate-Specific Antigen / Q000097:blood* / Q000235:genetics*; D011471:Prostatic Neoplasms / Q000097:blood* / Q000208:ethnology / Q000235:genetics*; D018570:Risk Assessment; D012307:Risk Factors
chemical_list: C549870:KLK3 protein, human; D007610:Kallikreins; D017430:Prostate-Specific Antigen