title: Intracerebroventricular administration of urotensin II regulates food intake and sympathetic nerve activity in brown adipose tissue.
abstract: To clarify the functional roles of urotensin II in regulating energy balance, we investigated the effects of a central infusion of urotensin II on food intake, uncoupling protein (UCP) 1 mRNA expression, temperature, and sympathetic nervous system activity in brown adipose tissue (BAT), a site that regulates energy expenditure in rodents. A bolus central infusion of urotensin II at a dose of 1 nmol/rat into the third cerebral ventricle decreased food intake (p<0.05). Additionally, urotensin II induced c-Fos-like-immunoreactivity (c-FLI) in the paraventricular nucleus (PVN) as compared with that in the control (phosphate buffered saline [PBS]-treated) group. Furthermore, urotensin II increased BAT UCP 1 mRNA expression (p<0.05). Finally, central infusion of urotensin II significantly increased BAT sympathetic nerve activity, which was accompanied by a significant elevation in BAT temperature (p<0.05) in rats. Taken together, central infusion of urotensin II regulates food intake and BAT sympathetic nerve activity in rats.
authors.affiliation: Department of Internal Medicine I, Faculty of Medicine, Oita University, 1-1, Idaigaoka, Hasama, Oita 879-5593, Japan.
authors.forename: Tohru
authors.initials: T
authors.lastname: Yasuda
authors.forename: Takayuki
authors.initials: T
authors.lastname: Masaki
authors.forename: Koro
authors.initials: K
authors.lastname: Gotoh
authors.forename: Seiichi
authors.initials: S
authors.lastname: Chiba
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Kakuma
authors.forename: Hironobu
authors.initials: H
authors.lastname: Yoshimatsu
mesh_terms: D000200:Action Potentials / Q000187:drug effects; D002001:Adipose Tissue, Brown / Q000187:drug effects / Q000294:innervation*; D000818:Animals; D001067:Appetite Depressants / Q000008:administration & dosage*; D001069:Appetite Regulation / Q000187:drug effects*; D002552:Cerebral Ventricles / Q000187:drug effects*; D015870:Gene Expression / Q000187:drug effects; D007473:Ion Channels / Q000235:genetics / Q000378:metabolism; D008297:Male; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism; D010286:Paraventricular Hypothalamic Nucleus / Q000187:drug effects / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013564:Sympathetic Nervous System / Q000187:drug effects*; D000071256:Uncoupling Protein 1; D014579:Urotensins / Q000008:administration & dosage*
chemical_list: D001067:Appetite Depressants; D007473:Ion Channels; D024101:Mitochondrial Proteins; D016760:Proto-Oncogene Proteins c-fos; C000604373:Ucp1 protein, rat; D000071256:Uncoupling Protein 1; D014579:Urotensins; C029677:urotensin II