title: Effects of selenium and light wavelengths on liquid culture of Cordyceps militaris Link.
abstract: To investigate the effects of selenium and light wavelengths on the growth of liquid-cultured Cordyceps militaris and the main active components' accumulation, culture conditions as selenium selenite concentrations and light of different wavelengths were studied. The results are: adenosine accumulation proved to be significantly selenium dependent (R(2) = 0.9403) and cordycepin contents were determined to be not significantly selenium dependent (R(2) = 0.3845) but significantly enhanced by selenium except for 20 ppm; there were significant differences in cordycepin contents, adenosine contents, and mycelium growth caused by light wavelengths: cordycepin, blue light > pink light > daylight, darkness, red light; adenosine, red light > pink light, darkness, daylight, blue light; and mycelium growth, red light > pink light, darkness, daylight > blue light. In conclusion, light wavelength had a significant influence on production of mycelia, adenosine, and cordycepin, so lightening wavelength should be changed according to target products in the liquid culture of C. militaris.
authors.affiliation: Key Laboratory of Biologic Resources Protection and Utilization of Hubei Province, School of Biological Science and Technology, Hubei University for Nationalities, Enshi, China. djz21cn@yahoo.com.cn
authors.forename: J Z
authors.initials: JZ
authors.lastname: Dong
authors.forename: M R
authors.initials: MR
authors.lastname: Liu
authors.forename: C
authors.initials: C
authors.lastname: Lei
authors.forename: X J
authors.initials: XJ
authors.lastname: Zheng
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000241:Adenosine / Q000378:metabolism; D032661:Cordyceps / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism / Q000528:radiation effects; D046508:Culture Techniques; D003839:Deoxyadenosines / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D008027:Light; D025282:Mycelium / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects; D012643:Selenium / Q000494:pharmacology*
chemical_list: D003839:Deoxyadenosines; C058120:cordycepin; D012643:Selenium; D000241:Adenosine