title: Gene expression profiling of a cold-shocked earthworm Eisenia andrei.
abstract: To identify genes that are modulated under cold-stress conditions in the earthworm Eisenia andrei, we performed a genome-wide analysis of gene expression in cold-shocked earthworms by using Serial Analysis of Gene Expression (SAGE). We identified 5,977 and 5,407 unique SAGE tags under normal and cold-stressed conditions, respectively. The majority of the SAGE tags did not match to any known expressed sequences, due to a paucity of expression data in earthworms. We converted the statistically significant SAGE tags for the cold-stressed condition into expressed sequence tags (ESTs), and the results showed that particular genes associated with energy homeostasis, cellular defense mechanisms, and ion balance were up-regulated or down-regulated. We constructed a regulatory network of some of these genes and identified rps-6 as a core gene in the cold-response regulatory-gene network. Our data provide a baseline for gene expression studies of cold shock in the Lumbricidae.
authors.affiliation: School of Life Science and Biotechnology, Kyungpook National University, Seoul, Republic of Korea.
authors.forename: H S
authors.initials: HS
authors.lastname: Kim
authors.forename: C H
authors.initials: CH
authors.lastname: Ahn
authors.forename: T S
authors.initials: TS
authors.lastname: Park
authors.forename: H D
authors.initials: HD
authors.lastname: Park
authors.forename: K S
authors.initials: KS
authors.lastname: Koh
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Ryoo
authors.forename: S C
authors.initials: SC
authors.lastname: Park
authors.forename: S
authors.initials: S
authors.lastname: Lee
mesh_terms: D000818:Animals; D001483:Base Sequence; D003080:Cold Temperature; D058639:Cold-Shock Response / Q000235:genetics*; D019295:Computational Biology; D004734:Energy Metabolism; D020224:Expressed Sequence Tags; D015870:Gene Expression; D020869:Gene Expression Profiling; D053263:Gene Regulatory Networks; D016678:Genome; D055106:Genome-Wide Association Study; D017136:Ion Transport; D046228:Microarray Analysis; D009835:Oligochaeta / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction