title: Proteome analysis of a CTR9 deficient yeast strain suggests that Ctr9 has function(s) independent of the Paf1 complex.
abstract: The Ctr9 protein is a member of the Paf1 complex implicated in multiple functions: transcription initiation and elongation by RNA pol II, RNA processing and histone modifications. It has also been described as a triple-helical DNA binding protein. Loss of Ctr9 results in severe phenotypes similar to the loss of Paf1p, a Paf1 complex subunit. However, the exact role of Ctr9 is not entirely established. To study the biological role of the protein Ctr9 in yeast, we used 2-D gel electrophoresis and characterized proteome alterations in a ctr9Δ mutant strain. Here we present results suggesting that Ctr9 has function distinct from its established role in the Paf1 complex. This role could be linked to its ability to bind to DNA complex structures as triplexes that may have function in regulation of gene expression.
authors.affiliation: UMR CNRS 5095, Bordeaux, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Massoni-Laporte
authors.forename: Michel
authors.initials: M
authors.lastname: Perrot
authors.forename: Loïc
authors.initials: L
authors.lastname: Ponger
authors.forename: Hélian
authors.initials: H
authors.lastname: Boucherie
authors.forename: Anne-Laure
authors.initials: AL
authors.lastname: Guieysse-Peugeot
mesh_terms: D018797:Cell Cycle Proteins / Q000172:deficiency / Q000235:genetics*; D004247:DNA; D004271:DNA, Fungal / Q000235:genetics* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D020543:Proteome; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism; D013194:Staining and Labeling; D013462:Sulfur Radioisotopes; D014158:Transcription, Genetic; D035602:Transcriptional Elongation Factors / Q000172:deficiency / Q000235:genetics*
chemical_list: C104718:CTR9 protein, S cerevisiae; D018797:Cell Cycle Proteins; D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C097698:PAF1 protein, S cerevisiae; D020543:Proteome; D029701:Saccharomyces cerevisiae Proteins; D013462:Sulfur Radioisotopes; D035602:Transcriptional Elongation Factors; C051802:triplex DNA; D004247:DNA