title: Specific labeling of neurogenic, endothelial, and myogenic differentiated cells derived from human amniotic fluid stem cells with silica-coated magnetic nanoparticles.
abstract: Stem cell based cell therapies offer significant potential for the field of regenerative medicine. Human amniotic fluid stem cells (hAFSCs) are an attractive source for lineage-specific differentiated stem cell therapy since they have properties that are able to differentiate into cells representing all three germ layers. To better understand the fate and location of implanted hAFSCs, a means to monitor cells in living subjects is essential. Here, we showed that differentiated cells, such as neurogenic, endothelial, and myogenic cells, derived from hAFSCs can be effectively labeled by the FITC-incorporated silica-coated nanoparticles, MNPs@SiO2 (FITC), although the labeling efficacy and cytotoxicity were distinct depending on the differentiated cell type. In addition, we observed that MNPs@SiO2-labeled cells provided sufficient signals for detection by optical and confocal microscope imaging when transplanted into the mice. These results suggest that the fluorescent dye incorporated MNPs@SiO2 are a useful tool for the cell labeling and in vivo tracking of differentiated cells derived from hAFSCs.
authors.affiliation: Stem Cell Neuroplasticity Research Group, Kyungpook National University, Daegu, Korea.
authors.forename: Jong Kil
authors.initials: JK
authors.lastname: Lee
authors.forename: So Young
authors.initials: SY
authors.lastname: Chun
authors.forename: Jin Young
authors.initials: JY
authors.lastname: Im
authors.forename: Hee Kyung
authors.initials: HK
authors.lastname: Jin
authors.forename: Tae Gyun
authors.initials: TG
authors.lastname: Kwon
authors.forename: Jae-Sung
authors.initials: JS
authors.lastname: Bae
mesh_terms: D000653:Amniotic Fluid / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D058948:Cell Tracking / Q000379:methods; D017690:Cell Transplantation; D042783:Endothelial Cells / Q000166:cytology / Q000502:physiology; D006801:Humans; D008280:Magnetics; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D032342:Muscle Cells / Q000166:cytology / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000502:physiology; D012822:Silicon Dioxide; D013234:Stem Cells / Q000502:physiology*
chemical_list: D012822:Silicon Dioxide