title: Comparative proteomic profiling of human lung adenocarcinoma cells (CL 1-0) expressing miR-372.
abstract: Lung cancer is a common malignancy and has a poor overall prognosis. Widespread metastasis is a common phenomenon in non-small cell lung cancer (NSCLC). It has been demonstrated that cancer relapse and survival can be predicted by the presence of a five-microRNA (miRNA) signature independent of stage or histologic type in NSCLC patients. Among the five miRNAs in the signature, miR-372 has been shown to play a significant role in metastasis and in the development of human testicular germ cell tumors. In addition, there is evidence that miR-372 posttranscriptionally downregulates large tumor suppressor, homolog 2 (Lats2), resulting in tumorigenesis and proliferation. To further investigate the cellular mechanisms involved in miR-372-induced silencing, we conducted a comparative proteomic analysis of NSCLC CL 1-0 cells expressing miRNA-372 and/or vector only by using two-dimensional gel electrophoresis (2DE), two-dimensional difference gel electrophoresis (2D-DIGE), and LC/MS/MS. Proteins identified as being up- or downregulated were further classified according to their biological functions. Many of the proteins identified in our study may be potential diagnostic biomarkers of NSCLC, particularly phosphorylated eIF4A-I.
authors.affiliation: Institute of Molecular Biology, National Chung Hsing University, Taichung, Taiwan.
authors.forename: Juo-Hsin
authors.initials: JH
authors.lastname: Lai
authors.forename: Tzu-Fang
authors.initials: TF
authors.lastname: She
authors.forename: Yu-Min
authors.initials: YM
authors.lastname: Juang
authors.forename: Yeou-Guang
authors.initials: YG
authors.lastname: Tsay
authors.forename: Ay-Huey
authors.initials: AH
authors.lastname: Huang
authors.forename: Sung-Liang
authors.initials: SL
authors.lastname: Yu
authors.forename: Jeremy J W
authors.initials: JJ
authors.lastname: Chen
authors.forename: Chien-Chen
authors.initials: CC
authors.lastname: Lai
mesh_terms: D000595:Amino Acid Sequence; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D015180:Electrophoresis, Gel, Two-Dimensional; D039601:Eukaryotic Initiation Factor-4A / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism*; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics; D020543:Proteome / Q000032:analysis; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry; D014162:Transfection
chemical_list: C561021:MIRN372 microRNA, human; D035683:MicroRNAs; D009363:Neoplasm Proteins; D020543:Proteome; D039601:Eukaryotic Initiation Factor-4A