title: Monoamine oxidase A gene DNA hypomethylation - a risk factor for panic disorder?
abstract: The monoamine oxidase A (MAOA) gene has been suggested as a prime candidate in the pathogenesis of panic disorder. In the present study, DNA methylation patterns in the MAOA regulatory and exon 1/intron 1 region were investigated for association with panic disorder with particular attention to possible effects of gender and environmental factors. Sixty-five patients with panic disorder (44 females, 21 males) and 65 healthy controls were analysed for DNA methylation status at 42 MAOA CpG sites via direct sequencing of sodium bisulfate treated DNA extracted from blood cells. The occurrence of recent positive and negative life events was ascertained. Male subjects showed no or only very minor methylation with some evidence for relative hypomethylation at one CpG site in intron 1 in patients compared to controls. Female patients exhibited significantly lower methylation than healthy controls at 10 MAOA CpG sites in the promoter as well as in exon/intron 1, with significance surviving correction for multiple testing at four CpG sites (p≤0.001). Furthermore, in female subjects the occurrence of negative life events was associated with relatively decreased methylation, while positive life events were associated with increased methylation. The present pilot data suggest a potential role of MAOA gene hypomethylation in the pathogenesis of panic disorder particularly in female patients, possibly mediating a detrimental influence of negative life events. Future studies are warranted to replicate the present finding in independent samples, preferably in a longitudinal design.
authors.affiliation: Department of Psychiatry, University of Muenster, Germany. Domschke_K@klinik.uni-wuerzburg.de
authors.forename: Katharina
authors.initials: K
authors.lastname: Domschke
authors.forename: Nicola
authors.initials: N
authors.lastname: Tidow
authors.forename: Henriette
authors.initials: H
authors.lastname: Kuithan
authors.forename: Kathrin
authors.initials: K
authors.lastname: Schwarte
authors.forename: Benedikt
authors.initials: B
authors.lastname: Klauke
authors.forename: Oliver
authors.initials: O
authors.lastname: Ambrée
authors.forename: Andreas
authors.initials: A
authors.lastname: Reif
authors.forename: Hartmut
authors.initials: H
authors.lastname: Schmidt
authors.forename: Volker
authors.initials: V
authors.lastname: Arolt
authors.forename: Anette
authors.initials: A
authors.lastname: Kersting
authors.forename: Peter
authors.initials: P
authors.lastname: Zwanzger
authors.forename: Jürgen
authors.initials: J
authors.lastname: Deckert
mesh_terms: D000328:Adult; D001483:Base Sequence; D019175:DNA Methylation / Q000235:genetics*; D039721:Diagnostic and Statistical Manual of Mental Disorders; D044127:Epigenesis, Genetic; D005091:Exons / Q000235:genetics; D005260:Female; D006801:Humans; D008016:Life Change Events; D008297:Male; D018598:Minisatellite Repeats / Q000235:genetics; D008969:Molecular Sequence Data; D008995:Monoamine Oxidase / Q000235:genetics*; D009153:Mutagens; D016584:Panic Disorder / Q000453:epidemiology* / Q000235:genetics* / Q000523:psychology; D010865:Pilot Projects; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics; D012307:Risk Factors; D012727:Sex Characteristics; D012907:Smoking / Q000235:genetics; D013447:Sulfites
chemical_list: D009153:Mutagens; D013447:Sulfites; D008995:Monoamine Oxidase; C009279:sodium bisulfite