title: Facile, rapid and efficient biofabrication of gold nanoparticles decorated with functional proteins.
abstract: We report a one-pot biological approach to fabricate gold nanoparticle (AuNP)-ZZ domain conjugates using peptide-functionalized proteins that can simultaneously direct both biomineralization and surface modification of AuNPs. In addition, immuno-AuNPs are readily prepared through the specific binding of antibodies to the ZZ domain on the AuNPs.
authors.affiliation: Division of Environment and Radiation Sciences, Nuclear Science and Engineering Directorate, Japan Atomic Energy Agency, Tokai-mura, Ibaraki 319-1195, Japan. shimojo.kojiro@jaea.go.jp
authors.forename: Kojiro
authors.initials: K
authors.lastname: Shimojo
authors.forename: Teppei
authors.initials: T
authors.lastname: Niide
authors.forename: Tomitsugu
authors.initials: T
authors.lastname: Taguchi
authors.forename: Hirochika
authors.initials: H
authors.lastname: Naganawa
authors.forename: Noriho
authors.initials: N
authors.lastname: Kamiya
authors.forename: Masahiro
authors.initials: M
authors.lastname: Goto
mesh_terms: D000595:Amino Acid Sequence; D000906:Antibodies / Q000276:immunology; D006046:Gold / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007118:Immunoassay; D053768:Metal Nanoparticles / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry* / Q000276:immunology; D011485:Protein Binding; D013499:Surface Properties
chemical_list: D000906:Antibodies; D010455:Peptides; D006046:Gold