title: Common nucleotide sequence of structural gene encoding fibroblast growth factor 4 in eight cattle derived from three breeds.
abstract: Fibroblast growth factor 4 (FGF4) is considered as a crucial gene for the proper development of bovine embryos. However, the complete nucleotide sequences of the structural genes encoding FGF4 in identified breeds are still unknown. In the present study, direct sequencing of PCR products derived from genomic DNA samples obtained from three Japanese Black, two Japanese Shorthorn and three Holstein cattle, revealed that the nucleotide sequences of the structural gene encoding FGF4 matched completely among these eight cattle. On the other hand, differences in the nucleotide sequences, leading to substitutions, insertions or deletions of amino acid residues were detected when compared with the already reported sequence from unidentified breeds. We cannot rule out a possibility that the structural gene elucidated in the present study is widely distributed in cattle. To the best of our knowledge, this is the first determination of the complete nucleotide sequence of the structural gene encoding bovine FGF4 in identified breeds.
authors.affiliation: Graduate School of Bioresource Sciences, Akita Prefectural University, Akita Livestock Experiment Station, Akita, Japan.
authors.forename: Sho
authors.initials: S
authors.lastname: Sato
authors.forename: Toshikiyo
authors.initials: T
authors.lastname: Takahashi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nishinomiya
authors.forename: Makiko
authors.initials: M
authors.lastname: Katoh
authors.forename: Ryu
authors.initials: R
authors.lastname: Itoh
authors.forename: Masaki
authors.initials: M
authors.lastname: Yokoo
authors.forename: Mari
authors.initials: M
authors.lastname: Yokoo
authors.forename: Momoe
authors.initials: M
authors.lastname: Iha
authors.forename: Yuki
authors.initials: Y
authors.lastname: Mori
authors.forename: Kano
authors.initials: K
authors.lastname: Kasuga
authors.forename: Ikuo
authors.initials: I
authors.lastname: Kojima
authors.forename: Masayuki
authors.initials: M
authors.lastname: Kobayashi
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics*; D005260:Female; D051519:Fibroblast Growth Factor 4 / Q000235:genetics*
chemical_list: D051519:Fibroblast Growth Factor 4