title: [Construction of electrochemiluminescence system for harmful algae detection].
abstract: To develop a new technique, Electrochemiluminescence-Molecular Probe (ECL-MP), for harmful algae detection, a highly sensitive electrochemiluminescence (ECL) detection system was set up based on the principle of ECL and related literature. The optimization tests were carried out, including the impact of voltage and current, the concentration of tripropylamine (TPrA) and the pH of phosphate buffer (PBS). The determination limit of Ru (bpy)3Cl2 x 6H2O was 10(-11) mol x L(-1) and the detection range was from 10(-9) - 10(-5) mol x L(-1) as well as the detection amount of substance was in the range from 0.4 pmol-4 nmol in the optimal reaction conditions with voltage of 1.0 V, current of 1.0 mA, TPrA concentration of 1.5 mol x L(-1) and pH of 7.4. It was proved by facts that this ECL analyzer was stable and highly sensitive, which was helpful in establishment of ECL-MP.
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China. myfavorite_1314@163.com
authors.forename: Xi
authors.initials: X
authors.lastname: Zhu
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhen
authors.forename: Tie-Zhu
authors.initials: TZ
authors.lastname: Mi
authors.forename: Zhen-Ming
authors.initials: ZM
authors.lastname: Chi
authors.forename: Xiao-Chun
authors.initials: XC
authors.lastname: Xu
mesh_terms: D055664:Electrochemical Techniques / Q000295:instrumentation; D004867:Equipment Design; D057097:Harmful Algal Bloom; D049449:Luminescence; D008163:Luminescent Measurements / Q000295:instrumentation*; D015336:Molecular Probe Techniques / Q000295:instrumentation*; D000461:Rhodophyta / Q000737:chemistry* / Q000235:genetics; D012428:Ruthenium / Q000737:chemistry
chemical_list: D012428:Ruthenium