title: Biomechanical and structural changes following the decellularization of bovine pericardial tissues for use as a tissue engineering scaffold.
abstract: To achieve natural scaffolds for tissue engineering applications we decellularized bovine pericardial (BP) tissues according to two different protocols: a novel treatment based on Triton(®) X-100 (12 h, 4 °C) (BP1) and a trypsin/EDTA treatment (37 °C, 48 h) (BP2). Results were compared with commercially available acellular xenogeneic biomaterials, Veritas(®) and Collamed(®). Biomechanical characteristics, high (E(h)) and low (E(l)) modulus of elasticity, of the fresh untreated tissue varied with the anatomical direction (apex to base (T) to transverse (L)) (mean ± SDEV): (41.63 ± 14.65-48.12 ± 10.19 MPa and 0.27 ± 0.05-0.30 ± 0.12 MPa respectively). BP1 had no mechanical effect (44.65 ± 19.73-52.67 ± 7.59 MPa and 0.37 ± 0.14-0.37 ± 0.11 MPa, respectively) but BP2 resulted in significant decrease in E(h) and E(l) (20.96 ± 8.17-36.82 ± 3.23 MPa and 0.20 ± 0.06-0.23 ± 0.06 MPa). Hysteresis ratio (h) varied (19-26 % of the loading energy) independently of anatomical direction. Glycosaminoglycans content was unaffected by BP1, while 22 % of chondroitin/dermatan sulphate and 60 % of hyaluronan were removed after BP2 treatment. Endothelial cell adhesion was achieved after 24 h and 3 days cell culture.
authors.affiliation: Department of Mechanical Engineering & Aer/tics, University of Patras, Patras, Greece.
authors.forename: Eirini
authors.initials: E
authors.lastname: Pagoulatou
authors.forename: Irene-Eva
authors.initials: IE
authors.lastname: Triantaphyllidou
authors.forename: Demitrios H
authors.initials: DH
authors.lastname: Vynios
authors.forename: Dionysios J
authors.initials: DJ
authors.lastname: Papachristou
authors.forename: Efstratios
authors.initials: E
authors.lastname: Koletsis
authors.forename: Despina
authors.initials: D
authors.lastname: Deligianni
authors.forename: Dimosthenis
authors.initials: D
authors.lastname: Mavrilas
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D001696:Biomechanical Phenomena; D001705:Bioprosthesis; D002417:Cattle; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002465:Cell Movement; D055119:Elastic Modulus; D042783:Endothelial Cells / Q000166:cytology; D006801:Humans; D008422:Materials Testing; D010496:Pericardium / Q000737:chemistry / Q000166:cytology; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials