title: Piperine inhibits lipopolysaccharide-induced maturation of bone-marrow-derived dendritic cells through inhibition of ERK and JNK activation.
abstract: Piperine, one of the main components of Piper longum Linn. and P. nigrum Linn., is a plant alkaloid with a long history of medicinal use. Piperine has been shown to modulate the immune response, but the mechanism underlying this modulation remains unknown. Here, we examined the effects of piperine on lipopolysaccharide (LPS)-induced inflammatory responses in bone-marrow-derived dendritic cells (BMDCs). Piperine significantly inhibited the expression of major histocompatibility complex class II, CD40 and CD86 in BMDCs in a dose-dependent manner. Furthermore, piperine treatment led to an increase in fluorescein-isothiocyanate-dextran uptake in LPS-treated dendritic cells and inhibited the production of tumour necrosis factor alpha and interleukin (IL)-12, but not IL-6. The inhibitory effects of piperine were mediated via suppression of extracellular signal-regulated kinases and c-Jun N-terminal kinases activation, but not p38 or nuclear factor-κB activation. These findings provide insight into the immunopharmacological role of piperine.
authors.affiliation: Hanbang Body-fluid Research Center, Wonkwang University, Iksan, 540-749 Jeonbuk, South Korea.
authors.forename: Gi-Sang
authors.initials: GS
authors.lastname: Bae
authors.forename: Jong-Jin
authors.initials: JJ
authors.lastname: Kim
authors.forename: Kyoung-Chel
authors.initials: KC
authors.lastname: Park
authors.forename: Bon Soon
authors.initials: BS
authors.lastname: Koo
authors.forename: Il-Joo
authors.initials: IJ
authors.lastname: Jo
authors.forename: Sun Bok
authors.initials: SB
authors.lastname: Choi
authors.forename: Chang Hyuk
authors.initials: CH
authors.lastname: Lee
authors.forename: Won-Seok
authors.initials: WS
authors.lastname: Jung
authors.forename: Jung-Hee
authors.initials: JH
authors.lastname: Cho
authors.forename: Seung-Heon
authors.initials: SH
authors.lastname: Hong
authors.forename: Ho-Joon
authors.initials: HJ
authors.lastname: Song
authors.forename: Yong Kook
authors.initials: YK
authors.lastname: Shin
authors.forename: Sung-Joo
authors.initials: SJ
authors.lastname: Park
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D052117:Benzodioxoles / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D003713:Dendritic Cells / Q000187:drug effects*; D007249:Inflammation / Q000378:metabolism; D018664:Interleukin-12 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D048031:JNK Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors*; D008070:Lipopolysaccharides; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D051379:Mice; D010766:Phosphorylation; D010880:Piperidines / Q000494:pharmacology*; D053284:Polyunsaturated Alkamides / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000470:Alkaloids; D052117:Benzodioxoles; D015850:Interleukin-6; D008070:Lipopolysaccharides; D010880:Piperidines; D053284:Polyunsaturated Alkamides; D014409:Tumor Necrosis Factor-alpha; D018664:Interleukin-12; D048031:JNK Mitogen-Activated Protein Kinases; C008922:piperine