title: Inter-species sequence comparison of Brachypodium reveals how transposon activity corrodes genome colinearity.
abstract: Intergenic sequences evolve rapidly in plant genomes through a process known as genomic turnover. To investigate the influence of DNA transposons on genomic turnover, we compared 1 Mbp of orthologous genomic sequences from Brachypodium distachyon and Brachypodium sylvaticum. We found that B. distachyon and B. sylvaticum diverged approximately 1.7-2.0 million years ago. Of a total of 219 genes identified on the analyzed sequences, 211 were colinear. However, only 24 transposable elements of a total of 451 were orthologous (i.e. inserted in the common ancestor). We characterized in detail 59 insertions and 60 excisions of DNA transposons in one or other species, which altered 17% of the intergenic space. The DNA transposon excision sites showed complex and highly diagnostic sequence motifs for double-strand break (DSB) repair. DNA transposon excisions can lead to extensive deletions of hundreds of base pairs of flanking sequence if the DSB is repaired by 'single-strand annealing', or insertions of up to several hundred base pairs of 'filler DNA' if the DSB is repaired by 'synthesis-dependent strand annealing'. In some cases, DSBs were repaired by a combination of both methods. We present a model for the evolution of intergenic sequences in which repair of DSBs upon DNA transposon excision is a major factor in the rapid turnover and erosion of intergenic sequences.
authors.affiliation: Institute of Plant Biology, University of Zurich, Zollikerstraße 107, 8008 Zurich, Switzerland.
authors.forename: Jan P
authors.initials: JP
authors.lastname: Buchmann
authors.forename: Takashi
authors.initials: T
authors.lastname: Matsumoto
authors.forename: Nils
authors.initials: N
authors.lastname: Stein
authors.forename: Beat
authors.initials: B
authors.lastname: Keller
authors.forename: Thomas
authors.initials: T
authors.lastname: Wicker
mesh_terms: D001483:Base Sequence; D005075:Biological Evolution; D058431:Brachypodium / Q000235:genetics*; D017124:Conserved Sequence; D004247:DNA; D004260:DNA Repair; D004251:DNA Transposable Elements; D021901:DNA, Intergenic; D019143:Evolution, Molecular; D018745:Genome, Plant; D008957:Models, Genetic; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D017384:Sequence Deletion; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004251:DNA Transposable Elements; D021901:DNA, Intergenic; D004247:DNA