title: 7-Nitroindazole potentiates c-fos expression induced by muscle tendon vibration in the spinal cord.
abstract: BACKGROUND
  Expression of c-fos initiated by muscle proprioceptive signaling was studied in rats after inhibition of neuronal nitric oxide synthase (nNOS) with administration of 7-nitroindazole (7-NI).
  
  
  METHODS
  Fos-immunoreactive (Fos-ir) neurons were visualized immunohistochemically in the lumbar cord after vibration of the Achilles tendon and/or 7-NI systemic injections.
  
  
  RESULTS
  The total number of Fos-ir interneurons and motoneurons (per slice) was significantly greater in the 7-NI-pretreated and tendon-vibrated (7-NI + Tv) group than in the isolated tendon vibration group (Tv group). The greatest increases in the number of Fos-ir neurons were found in the L4 (+100%) and L5 (+105%) segments (P < 0.05).
  
  
  CONCLUSIONS
  Suppression of NO release after introduction of 7-NI was associated with potentiation of Fos immunoreactivity induced by muscle proprioceptive signaling within distinctive regions of the spinal cord.
authors.affiliation: Department of Movement Physiology, Bogomoletz Institute of Physiology, National Academy of Sciences, Bogomoletz Str. 4, Kiev 01024, Ukraine. pil@biph.kiev.ua
authors.forename: Alexander I
authors.initials: AI
authors.lastname: Pilyavskii
authors.forename: Vladimir A
authors.initials: VA
authors.lastname: Maisky
authors.forename: Andrey V
authors.initials: AV
authors.lastname: Maznychenko
authors.forename: Alexander I
authors.initials: AI
authors.lastname: Kostyukov
mesh_terms: D000125:Achilles Tendon / Q000502:physiology; D000818:Animals; D002452:Cell Count; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects* / Q000502:physiology*; D016762:Genes, fos / Q000187:drug effects*; D007150:Immunohistochemistry; D007191:Indazoles / Q000494:pharmacology*; D007274:Injections, Intraperitoneal; D007395:Interneurons / Q000502:physiology; D008297:Male; D009046:Motor Neurons / Q000502:physiology; D018482:Muscle, Skeletal / Q000502:physiology; D009475:Neurons, Afferent / Q000378:metabolism / Q000502:physiology; D052248:Nitric Oxide Synthase Type I / Q000037:antagonists & inhibitors*; D011434:Proprioception / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D013116:Spinal Cord / Q000502:physiology*; D013710:Tendons / Q000502:physiology*; D014732:Vibration
chemical_list: D004791:Enzyme Inhibitors; D007191:Indazoles; D052248:Nitric Oxide Synthase Type I; C080122:7-nitroindazole