title: A device for the collection of submandibular saliva.
abstract: The objective of this study was to describe the construction of a non-invasive device for the collection of submandibular saliva. Preliminary tests were carried out on saliva collected from a single donor in order to determine whether the rheological properties of submandibular saliva collected using the device were comparable to whole saliva collected using the expectoration (or 'spit') method. The device collected a lower quantity of saliva than that collected using the expectoration method. Stimulated saliva collected using the device had a pH close to that of unstimulated saliva because the sealed collection unit in the device minimised contamination. Saliva exhibited shear-thinning behaviour regardless of the method of collection, although that collected using the device was more viscous. The viscoelasticity of saliva collected using the two methods was different, probably as a result of differences in composition. This difference was greater with stimulated saliva. Despite the discrepancies between whole saliva and submandibular saliva, the device provides a non-invasive method for the collection of high-quality saliva over extended periods.
authors.affiliation: School of Pharmacy, University of Otago, Dunedin, New Zealand. sara.hanning@otago.ac.nz
authors.forename: Sara
authors.initials: S
authors.lastname: Hanning
authors.forename: Lidia
authors.initials: L
authors.lastname: Motoi
authors.forename: Natalie
authors.initials: N
authors.lastname: Medlicott
authors.forename: Stephen
authors.initials: S
authors.lastname: Swindells
mesh_terms: D019343:Citric Acid / Q000494:pharmacology; D003760:Dental Impression Materials / Q000737:chemistry; D003761:Dental Impression Technique; D055119:Elastic Modulus; D004867:Equipment Design; D006801:Humans; D057446:Hydrodynamics; D006863:Hydrogen-Ion Concentration; D007440:Intubation / Q000295:instrumentation; D012117:Resins, Synthetic / Q000737:chemistry; D012212:Rheology; D012463:Saliva / Q000187:drug effects / Q000378:metabolism*; D018987:Salivary Ducts / Q000378:metabolism; D013048:Specimen Handling / Q000295:instrumentation / Q000379:methods*; D013193:Stainless Steel / Q000737:chemistry; D013363:Submandibular Gland / Q000187:drug effects / Q000378:metabolism*; D013526:Surgical Mesh; D014783:Viscosity
chemical_list: D003760:Dental Impression Materials; C022821:Impregum; D012117:Resins, Synthetic; D013193:Stainless Steel; D019343:Citric Acid