title: [Effect of arctiin on mouse podocyte epithelial-mesenchymal transition induced by advanced oxidation protein products].
abstract: OBJECTIVE
  To study the effect of arctiin on mouse podocyte epithelial-mesenchymal transition (EMT) induced by advanced oxidation protein products (AOPP).
  
  
  METHODS
  Mouse podocytes were stimulated by 200 µg/ml AOPP for 24 h in the presence of 50, 100, 200, and 400 µmol/L arctiin. The expressions of α-smooth muscle actin, Grp78 and CHOP were detected using Western blotting.
  
  
  RESULTS
  The expressions of α-SMA, Grp78 and CHOP were inhibited by arctiin, showing a dose-dependent effect within a given range of arctiin concentration.
  
  
  CONCLUSIONS
  AOPP causes endoplasmic reticulum stress to induce EMT of mouse podocytes, and arctiin can decrease EMT by alleviating the stress. This finding sheds light on a new scope of research of renal fibrosis.
authors.affiliation: Department of Nephrology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Guo
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.forename: Qing-Sheng
authors.initials: QS
authors.lastname: Du
authors.forename: Jie
authors.initials: J
authors.lastname: Hua
authors.forename: Rong-Zhi
authors.initials: RZ
authors.lastname: Liu
authors.forename: Xun
authors.initials: X
authors.lastname: Tang
mesh_terms: D000199:Actins / Q000378:metabolism; D062107:Advanced Oxidation Protein Products / Q000009:adverse effects*; D000818:Animals; D002460:Cell Line; D000091342:Endoplasmic Reticulum Chaperone BiP; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects*; D005663:Furans / Q000494:pharmacology*; D005960:Glucosides / Q000494:pharmacology*; D006360:Heat-Shock Proteins / Q000378:metabolism; D051379:Mice; D050199:Podocytes / Q000378:metabolism / Q000473:pathology*; D051742:Transcription Factor CHOP / Q000378:metabolism
chemical_list: C558396:Acta2 protein, mouse; D000199:Actins; D062107:Advanced Oxidation Protein Products; C497250:Ddit3 protein, mouse; D000091342:Endoplasmic Reticulum Chaperone BiP; D005663:Furans; D005960:Glucosides; D006360:Heat-Shock Proteins; C000717248:Hspa5 protein, mouse; D051742:Transcription Factor CHOP; C077992:arctiin