title: Antimicrobial susceptibility and molecular typing of multiple Chlamydiaceae species isolated from genital infection of women in Egypt.
abstract: This study investigated the existence of vaginal Chlamydia infection and the prevalence of the disease in symptomatic gynecologically diseased women in Egypt. In addition, the antibiotics penicillin, tetracycline, and erythromycin were evaluated for their in vitro antichlamydial activity of the isolated strains. Vaginal swabs (n=160) were collected from females gynecologically diseased using cotton swabs. Samples were tested for Chlamydia by Vero cells tissue culture, chicken embryo, Gimenez staining, direct fluorescein-conjugated monoclonal antibody staining, and immunoperoxidase. Polymerase chain reaction (PCR) analyses conducted for the presence of chlamydial DNA was used to detect its specific DNA by the omp2 gene. PCR analyses conducted for the presence of chlamydial DNA revealed that 112/160 (70%) were positive for Chlamydiaceae. The specific DNA defined by the omp2 gene identified them as Chlamydia trachomatis (17/112, 15.2%), Chlamydophila psittaci (56/112, 50.0%), and Chlamydophila abortus (40/112, 35.7%). The antibiotics penicillin, tetracycline, and erythromycin at different concentrations were effective in inactivating the viability of Chlamydiaceae isolates.
authors.affiliation: Department of Microbiology, Cairo University, Cairo, Egypt. kamelia-osman@hotmail.com
authors.forename: Kamelia M
authors.initials: KM
authors.lastname: Osman
authors.forename: Hadia A
authors.initials: HA
authors.lastname: Ali
authors.forename: Jakeen A
authors.initials: JA
authors.lastname: Eljakee
authors.forename: Maha M
authors.initials: MM
authors.lastname: Gaafar
authors.forename: Hussein M
authors.initials: HM
authors.lastname: Galal
mesh_terms: D000328:Adult; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015373:Bacterial Typing Techniques; D002642:Chick Embryo; D002690:Chlamydia Infections / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology*; D002693:Chlamydiaceae / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D002522:Chlorocebus aethiops; D004534:Egypt / Q000453:epidemiology; D004917:Erythromycin / Q000494:pharmacology*; D005260:Female; D006801:Humans; D008826:Microbial Sensitivity Tests; D010406:Penicillins / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D015995:Prevalence; D013752:Tetracycline / Q000494:pharmacology*; D014621:Vagina / Q000187:drug effects / Q000382:microbiology; D014709:Vero Cells
chemical_list: D000900:Anti-Bacterial Agents; D010406:Penicillins; D004917:Erythromycin; D013752:Tetracycline