title: Fgl2 prothrombinase is involved in severe acute pancreatitis-associated liver injury.
abstract: OBJECTIVE
  Severe acute pancreatitis (SAP)-associated liver injury is systematically one of main pathophysiological events due to SAP development. The aim of the study was to investigate whether fgl2 prothrombinase is involved in SAP-associated liver injury.
  
  
  METHODS
  Microthrombosis in the liver of rats with SAP was observed by Masson staining. Fgl2 prothrombinase expression in the liver of rats with SAP was analyzed by real-time PCR and immunohistochemistry methods.
  
  
  RESULTS
  Fgl2 prothrombinase gene and protein expression in SAP group were significantly up-regulated compared to sham-operation (SO) group. Immunohistochemistry staining showed that fgl2 prothrombinase was localized speci?cally to the endothelial cells of intrahepatic veins and hepatic sinusoids. Furthermore, Masson staining demonstrated that the proportion of hepatic microthrombotic capillaries in SAP group were evidently increasing in comparison to SO group and closely correlated with fgl2 expression (r=0.948, p<0.01 ). In addition, there was a positive correlation between fgl2 expression and the severity of hepatocellular injury as indicated by hepatic pathological grade (r=0.704, p<0.01).
  
  
  CONCLUSIONS
  Fgl2 prothrombinase may contribute to microthrombosis in SAP-associated liver injury, thus resulting in hepatic microcirculatory disturbance and measurement of fgl2 may be used as a helpful biomarker in the prognosis of the severity of hepatic pathological injury in SAP.
authors.affiliation: Department of Gastroenterology and Hepatology, Wenzhou Medical College, Wenzhou, China.
authors.forename: Tanzhou
authors.initials: T
authors.lastname: Chen
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Ye
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Huang
authors.forename: Renpin
authors.initials: R
authors.lastname: Chen
authors.forename: Xiaoju
authors.initials: X
authors.lastname: Zhuge
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.forename: Yong
authors.initials: Y
authors.lastname: Du
mesh_terms: D000208:Acute Disease; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D007150:Immunohistochemistry; D008099:Liver / Q000473:pathology; D008107:Liver Diseases / Q000209:etiology*; D008297:Male; D010195:Pancreatitis / Q000150:complications*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013925:Thromboplastin / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D013927:Thrombosis / Q000209:etiology
chemical_list: D013925:Thromboplastin; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase