title: Adult-brain-derived neural stem cells grafting into a vein bridge increases postlesional recovery and regeneration in a peripheral nerve of adult pig.
abstract: We attempted transplantation of adult neural stem cells (ANSCs) inside an autologous venous graft following surgical transsection of nervis cruralis with 30 mm long gap in adult pig. The transplanted cell suspension was a primary culture of neurospheres from adult pig subventricular zone (SVZ) which had been labeled in vitro with BrdU or lentivirally transferred fluorescent protein. Lesion-induced loss of leg extension on the thigh became definitive in controls but was reversed by 45-90 days after neurosphere-filled vein grafting. Electromyography showed stimulodetection recovery in neurosphere-transplanted pigs but not in controls. Postmortem immunohistochemistry revealed neurosphere-derived cells that survived inside the venous graft from 10 to 240 post-lesion days and all displayed a neuronal phenotype. Newly formed neurons were distributed inside the venous graft along the severed nerve longitudinal axis. Moreover, ANSC transplantation increased CNPase expression, indicating activation of intrinsic Schwann cells. Thus ANSC transplantation inside an autologous venous graft provides an efficient repair strategy.
authors.affiliation: Unité de Chirurgie et Physiologie Expérimentale (UCPE), Institut de Médecine Tropicale, 58 boulevard Charles Livon, 13007 Marseille, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Liard
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Segura
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Sagui
authors.forename: André
authors.initials: A
authors.lastname: Nau
authors.forename: Aurélie
authors.initials: A
authors.lastname: Pascual
authors.forename: Melissa
authors.initials: M
authors.lastname: Cambon
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Darlix
authors.forename: Thierry
authors.initials: T
authors.lastname: Fusai
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Moyse