title: The relationship between ER-multivesicular body membrane contacts and the ESCRT machinery.
abstract: Activated EGFR (epidermal growth factor receptor) undergoes ESCRT (endosomal sorting complex required for transport)-mediated sorting on to ILVs (intraluminal vesicles) of endosomes before degradation in the lysosome. Sorting of endocytosed EGFR on to ILVs removes the catalytic domain of the EGFR from the cytoplasm, resulting in termination of receptor signalling. EGFR signalling is also subject to down-regulation through receptor dephosphorylation by the ER (endoplasmic reticulum)-localized PTP1B (protein tyrosine phosphatase 1B). PTP1B on the cytoplasmic face of the ER interacts with endocytosed EGFR via direct membrane contacts sites between the ER and endosomes. In the present paper, we review the relationship between ER-endosome membrane contact sites and ILV formation, and their potential role in the regulation of EGFR sorting on to ILVs, through PTP1B-mediated dephosphorylation of both EGFR and components of the ESCRT machinery.
authors.affiliation: UCL Institute of Ophthalmology, University College London, 11-43 Bath Street, London EC1V 9EL, U.K.
authors.forename: Emily R
authors.initials: ER
authors.lastname: Eden
authors.forename: Thomas
authors.initials: T
authors.lastname: Burgoyne
authors.forename: James R
authors.initials: JR
authors.lastname: Edgar
authors.forename: Alexander
authors.initials: A
authors.lastname: Sorkin
authors.forename: Clare E
authors.initials: CE
authors.lastname: Futter
mesh_terms: D000818:Animals; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism*; D056827:Endosomal Sorting Complexes Required for Transport / Q000187:drug effects / Q000378:metabolism*; D004815:Epidermal Growth Factor / Q000494:pharmacology; D006801:Humans; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism*; D057146:Multivesicular Bodies / Q000187:drug effects / Q000378:metabolism*; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1 / Q000378:metabolism
chemical_list: D056827:Endosomal Sorting Complexes Required for Transport; D004815:Epidermal Growth Factor; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1