title: A lineage of myeloid cells independent of Myb and hematopoietic stem cells.
abstract: Macrophages and dendritic cells (DCs) are key components of cellular immunity and are thought to originate and renew from hematopoietic stem cells (HSCs). However, some macrophages develop in the embryo before the appearance of definitive HSCs. We thus reinvestigated macrophage development. We found that the transcription factor Myb was required for development of HSCs and all CD11b(high) monocytes and macrophages, but was dispensable for yolk sac (YS) macrophages and for the development of YS-derived F4/80(bright) macrophages in several tissues, such as liver Kupffer cells, epidermal Langerhans cells, and microglia--cell populations that all can persist in adult mice independently of HSCs. These results define a lineage of tissue macrophages that derive from the YS and are genetically distinct from HSC progeny.
authors.affiliation: Centre for Molecular and Cellular Biology of Inflammation, New Hunt's House, King's College London, Great Maze Pond, London SE1 1UL, UK.
authors.forename: Christian
authors.initials: C
authors.lastname: Schulz
authors.forename: Elisa
authors.initials: E
authors.lastname: Gomez Perdiguero
authors.forename: Laurent
authors.initials: L
authors.lastname: Chorro
authors.forename: Heather
authors.initials: H
authors.lastname: Szabo-Rogers
authors.forename: Nicolas
authors.initials: N
authors.lastname: Cagnard
authors.forename: Katrin
authors.initials: K
authors.lastname: Kierdorf
authors.forename: Marco
authors.initials: M
authors.lastname: Prinz
authors.forename: Bishan
authors.initials: B
authors.lastname: Wu
authors.forename: Sten Eirik W
authors.initials: SE
authors.lastname: Jacobsen
authors.forename: Jeffrey W
authors.initials: JW
authors.lastname: Pollard
authors.forename: Jon
authors.initials: J
authors.lastname: Frampton
authors.forename: Karen J
authors.initials: KJ
authors.lastname: Liu
authors.forename: Frederic
authors.initials: F
authors.lastname: Geissmann
mesh_terms: D000818:Animals; D019070:Cell Lineage; D049109:Cell Proliferation; D002642:Chick Embryo; D003713:Dendritic Cells / Q000166:cytology* / Q000502:physiology; D004622:Embryo, Mammalian / Q000166:cytology; D017353:Gene Deletion; D018507:Gene Expression Regulation, Developmental; D020570:Genes, myb; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000502:physiology; D007728:Kupffer Cells / Q000166:cytology / Q000502:physiology; D007801:Langerhans Cells / Q000166:cytology / Q000502:physiology; D008099:Liver / Q000196:embryology; D008264:Macrophages / Q000166:cytology* / Q000502:physiology; D051379:Mice; D017628:Microglia / Q000166:cytology / Q000502:physiology; D022423:Myeloid Cells / Q000166:cytology* / Q000502:physiology; D038042:Myelopoiesis; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism; D020598:Proto-Oncogene Proteins c-myb / Q000378:metabolism*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D015017:Yolk Sac / Q000166:cytology*; D000099232:Proto-Oncogene Protein Spi-1
chemical_list: D011518:Proto-Oncogene Proteins; D020598:Proto-Oncogene Proteins c-myb; D015534:Trans-Activators; D000099232:Proto-Oncogene Protein Spi-1