title: Pseudomonas fluorescens HK44: lessons learned from a model whole-cell bioreporter with a broad application history.
abstract: Initially described in 1990, Pseudomonas fluorescens HK44 served as the first whole-cell bioreporter genetically endowed with a bioluminescent (luxCDABE) phenotype directly linked to a catabolic (naphthalene degradative) pathway. HK44 was the first genetically engineered microorganism to be released in the field to monitor bioremediation potential. Subsequent to that release, strain HK44 had been introduced into other solids (soils, sands), liquid (water, wastewater), and volatile environments. In these matrices, it has functioned as one of the best characterized chemically-responsive environmental bioreporters and as a model organism for understanding bacterial colonization and transport, cell immobilization strategies, and the kinetics of cellular bioluminescent emission. This review summarizes the characteristics of P. fluorescens HK44 and the extensive range of its applications with special focus on the monitoring of bioremediation processes and biosensing of environmental pollution.
authors.affiliation: Faculty of Environment, Jan Evangelista Purkyně University in Ústí nad Labem, Králova Výšina 3132/7, 400 96 Ústí nad Labem, Czech Republic. josef.trogl@ujep.cz
authors.forename: Josef
authors.initials: J
authors.lastname: Trögl
authors.forename: Archana
authors.initials: A
authors.lastname: Chauhan
authors.forename: Steven
authors.initials: S
authors.lastname: Ripp
authors.forename: Alice C
authors.initials: AC
authors.lastname: Layton
authors.forename: Gabriela
authors.initials: G
authors.lastname: Kuncová
authors.forename: Gary S
authors.initials: GS
authors.lastname: Sayler
mesh_terms: D001681:Biological Assay / Q000295:instrumentation*; D015374:Biosensing Techniques / Q000295:instrumentation*; D004784:Environmental Monitoring / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D011551:Pseudomonas fluorescens / Q000187:drug effects* / Q000502:physiology*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*