title: Expression of microRNA-122 contributes to apoptosis in H9C2 myocytes.
abstract: The microRNAs (miRNAs) can post-transcriptionally regulate gene expression and heart development. The Pax-8 gene knockout mice have apparent heart abnormalities. This study investigated the role of miRNAs in regulation of cardiac apoptosis and development in the knockout mice. MicroRNA microarrays demonstrated differential expression of microRNAs between Pax-8(-/-)  and Pax-8(+/-)  mice, confirmed by real-time PCR. The miR-122 was up-regulated by 1.92 folds in Pax-8(-/-)  mice. There were ventricular septum defects in Pax-8(-/-)  mice, and increased numbers of apoptotic cells in the left ventricular wall and interventricular septum in Pax-8(-/-)  mice. In H9C2 myocytes, treatment with miR-122 mimics or miR-122 inhibitor affects the expression of CCK-8 and activity of Caspase-3. The miR-122 is up-regulated in the myocytes of Pax-8(-/-)  mice and may participate in the apoptotic gene expression and pathogenesis of heart development defect.
authors.affiliation: Division of Cardiology, The First Affiliated Hospital of Wenzhou Medical College, Wenzhou, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Huang
authors.forename: Fang
authors.initials: F
authors.lastname: Huang
authors.forename: Deye
authors.initials: D
authors.lastname: Yang
authors.forename: Fengquan
authors.initials: F
authors.lastname: Dong
authors.forename: Xiangxiang
authors.initials: X
authors.lastname: Shi
authors.forename: Hongyu
authors.initials: H
authors.lastname: Wang
authors.forename: Xi
authors.initials: X
authors.lastname: Zhou
authors.forename: Suyun
authors.initials: S
authors.lastname: Wang
authors.forename: Shengchuan
authors.initials: S
authors.lastname: Dai
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D018507:Gene Expression Regulation, Developmental; D006343:Heart Septal Defects / Q000235:genetics / Q000473:pathology; D006346:Heart Septum / Q000473:pathology; D051379:Mice; D018345:Mice, Knockout; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics*; D032383:Myocytes, Cardiac / Q000473:pathology* / Q000502:physiology; D000072636:PAX8 Transcription Factor; D051761:Paired Box Transcription Factors / Q000235:genetics; D034741:RNA, Small Interfering; D012844:Sincalide / Q000235:genetics / Q000378:metabolism; D014161:Transduction, Genetic; D015854:Up-Regulation
chemical_list: D035683:MicroRNAs; C544354:Mirn122 microRNA, mouse; D000072636:PAX8 Transcription Factor; D051761:Paired Box Transcription Factors; C499496:Pax8 protein, mouse; D034741:RNA, Small Interfering; C505409:Casp3 protein, mouse; D053148:Caspase 3; D012844:Sincalide