title: Heparin interacting protein mediated assembly of nano-fibrous hydrogel scaffolds for guided stem cell differentiation.
abstract: A new methodology is developed to conjugate hyaluronic acid (HA) hydrogel with novel nano-fibrous architectures via non-covalent assembly that specifically allows for targeted adipose-derived stem cells (ASCs) differentiation and soft tissue engineering. The assembly of non-covalently associated hydrogel network produced via the interaction of a low molecular weight heparin (LMWH) modified HA derivative and heparin interacting protein (HIP). The multifunctional star poly(ethylene glycol) (PEG) and HIP copolymer has the capability to mediate the non-covalent assembly of nano-fibrous HA hydrogel networks via affinity interactions with LMWH. The effect of the HIP mediation on in vitro gelation, rheological characteristics, degradation, equilibrium swelling, adipose-derived stem cells (ASCs) proliferation and differentiation of nano-fibrous hydrogel is examined. The results suggest the potential utility of this unique design of the bioactive nano-fibrous HA hydrogel in directing the differentiation of ASCs and adipogenesis in ECM-mimetic scaffolds in vitro. These studies demonstrate that this nano-fibrous HA hydrogel can render the formulation of a therapeutically effective platform for in vitro adipogenesis applications.
authors.affiliation: School of Materials Science and Engineering, Nanjing University of Science and Technology, Nanjing 210094, China. hptan@mail.njust.edu.cn
authors.forename: Huaping
authors.initials: H
authors.lastname: Tan
authors.forename: Qixin
authors.initials: Q
authors.lastname: Zhou
authors.forename: Haifeng
authors.initials: H
authors.lastname: Qi
authors.forename: Dan
authors.initials: D
authors.lastname: Zhu
authors.forename: Xiaoxue
authors.initials: X
authors.lastname: Ma
authors.forename: Dangsheng
authors.initials: D
authors.lastname: Xiong
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D001779:Blood Coagulation Factors / Q000737:chemistry* / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D005109:Extracellular Matrix / Q000737:chemistry; D006495:Heparin, Low-Molecular-Weight / Q000737:chemistry*; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry* / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D011092:Polyethylene Glycols / Q000737:chemistry; D016601:RNA-Binding Proteins; D012269:Ribosomal Proteins; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D001779:Blood Coagulation Factors; D006495:Heparin, Low-Molecular-Weight; D020100:Hydrogels; D016601:RNA-Binding Proteins; C104946:RPL29 protein, human; D012269:Ribosomal Proteins; D011092:Polyethylene Glycols; D006820:Hyaluronic Acid