title: Chloride intracellular channel 1 is an important factor in the lymphatic metastasis of hepatocarcinoma.
abstract: We have previously demonstrated that chloride intracellular channel 1 (CLIC1) is involved in the lymphatic metastasis of tumors. In this study, a self-designed shRNA sequence of mouse CLIC1 gene was synthesized and inserted into a pGPU6/GFP/Neo plasmid, then stably transfected into mouse hepatic carcinoma cell line Hca-F cells to down-regulate the expression of CLIC1 gene. The levels of expression of CLIC1 mRNA and protein were detected by real-time quantitative polymerase chain reaction (qRT-PCR) and western blot (WB) analysis, respectively. The down-regulation of CLIC1 enhanced proliferative activity, increased the ratio of G2/M and decreased percentage of apoptosis. In addition, the capability of migration and invasion decreased significantly. The results indicate that CLIC1 is a critical factor in the development of lymphatic metastasis.
authors.affiliation: Department of Gastroenterology and hepatology, the 2nd Affiliate Hospital of Dalian Medical University, Dalian, Liaoning Province, China.
authors.forename: Rong-Kuan
authors.initials: RK
authors.lastname: Li
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Yu-Hong
authors.initials: YH
authors.lastname: Zhang
authors.forename: Miao-Ling
authors.initials: ML
authors.lastname: Li
authors.forename: Mei
authors.initials: M
authors.lastname: Wang
authors.forename: Jian-Wu
authors.initials: JW
authors.lastname: Tang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology*; D002455:Cell Division / Q000235:genetics; D048708:Cell Growth Processes / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D018118:Chloride Channels / Q000235:genetics*; D015536:Down-Regulation; D016195:G2 Phase / Q000235:genetics; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology*; D008207:Lymphatic Metastasis; D051379:Mice; D009361:Neoplasm Invasiveness / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D014162:Transfection / Q000379:methods
chemical_list: D018118:Chloride Channels; C501967:Clic1 protein, mouse; D012333:RNA, Messenger; D034741:RNA, Small Interfering