title: miR-20a promotes migration and invasion by regulating TNKS2 in human cervical cancer cells.
abstract: miR-20a is an important member of the miR-17-92 cluster, and its real function in cervical cancer cells is unknown. Our study demonstrated that miR-20a was upregulated in cervical cancer tissues. Overexpression of miR-20a in cervical cancer-derived cell lines, HeLa and C-33A, enhanced long-term cellular proliferation, migration and invasion, whereas inhibition of miR-20a suppressed those functions. We also confirmed that oncogenic TNKS2 is directly upregulated by miR-20a. Furthermore, suppression of TNKS2 expression could inhibit colony formation, migration and invasion of cervical cancer cells. Therefore, we concluded that miR-20a can promote migration and invasion of cervical cancer cells through the upregulation of TNKS2.
authors.affiliation: Tianjin Life Science Research Center and Basic Medical School, Tianjin Medical University, Tianjin 300070, China.
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Kang
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Qian
authors.initials: Q
authors.lastname: Wei
authors.forename: Yi-Fan
authors.initials: YF
authors.lastname: Zhao
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.forename: Hua
authors.initials: H
authors.lastname: Tang
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness / Q000473:pathology*; D038501:Tankyrases / Q000235:genetics / Q000378:metabolism*; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology
chemical_list: D020413:3' Untranslated Regions; C534451:MIRN20a microRNA, human; D035683:MicroRNAs; C437334:TNKS2 protein, human; D038501:Tankyrases