title: Antioxidant activity of polyphenol and ultrasonic extracts from fruits of Crataegus pentagyna subsp. elburensis.
abstract: The effects of two extracting methods on the total phenolic and total flavonoid contents of Crataegus pentagyna subsp. elburensis Waldst. & Kit. ex Willd fruit extracts were investigated. Antioxidant activities of polyphenol (PP) fraction and ultrasonic (US) extraction were evaluated with four different in vitro antioxidant tests. IC(50) for DPPH radical-scavenging activity was 32.2 ± 1.6 for PP fraction and 36.7 ± 1.5 µg mL(-1) for US extract. Reducing powers of extracts increased with the increase of their concentrations. PP fraction exhibited high reducing power at 2-32 µg mL(-1). Extracts exhibited good H(2)O(2) radical scavenging and Fe(2+) chelating ability. Their high phenolic and flavonoid contents could be responsible for their antioxidant activity and pharmacologic actions.
authors.affiliation: Pharmaceutical Research Center, School of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Kh
authors.initials: Kh
authors.lastname: Rabiei
authors.forename: S
authors.initials: S
authors.lastname: Bekhradnia
authors.forename: S M
authors.initials: SM
authors.lastname: Nabavi
authors.forename: S F
authors.initials: SF
authors.lastname: Nabavi
authors.forename: M A
authors.initials: MA
authors.lastname: Ebrahimzadeh
mesh_terms: D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000378:metabolism; D027825:Crataegus / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000378:metabolism; D020128:Inhibitory Concentration 50; D007502:Iron Chelating Agents / Q000302:isolation & purification / Q000494:pharmacology; D010851:Picrates / Q000378:metabolism; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D059808:Polyphenols / Q000302:isolation & purification / Q000494:pharmacology*; D019163:Reducing Agents / Q000302:isolation & purification / Q000494:pharmacology; D014465:Ultrasonics
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D007502:Iron Chelating Agents; D010851:Picrates; D010936:Plant Extracts; D059808:Polyphenols; D019163:Reducing Agents; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl