title: [The number of circulating fibrocytes of skeletal muscle in rats after contusion].
abstract: OBJECTIVE
  To investigate the time-dependent appearance of circulating fibrocytes of skeletal muscle in rats after contusion.
  
  
  METHODS
  The model of skeletal muscle wound was established in rat. The circulating fibrocytes in contused skeletal muscle were detected by CD45 and procollagen I double immunofluorescence staining method.
  
  
  RESULTS
  In the control group, CD45- and procollagen I-positive cells were not detected in skeletal muscle. A few CD45 cells were observed aged from 6 h to 1 d after contusion. A few CD45- and procollagen I-positive cells (fibrocytes) initially gathered in injury area 3d after injury. The ratio of positive fibrocytes significantly increased 5 d after injury. The ratio of fibrocytes was highest at 7 d after contusion and then decreased. The volume of fibrocytes showed bigger with injury time increase compared with 3 d group. The expression of procollagen I and CD45 were weakened at 14d after injury.
  
  
  CONCLUSIONS
  The circulating fibrocytes are detected in contused skeletal muscle in time-dependent pattern. Circulating fibrocytes may be a marker in the wound age determination for contused skeletal muscle.
authors.affiliation: Department of Forensic Pathology, School of Forensic Medicine, China Medical University, Shenyang 110001, China.
authors.forename: Li-Qiang
authors.initials: LQ
authors.lastname: Li
authors.forename: Tian-Shui
authors.initials: TS
authors.lastname: Yu
authors.forename: Da-Wei
authors.initials: DW
authors.lastname: Guan
authors.forename: Yan-Yan
authors.initials: YY
authors.lastname: Fan
authors.forename: Rui
authors.initials: R
authors.lastname: Zhao
authors.forename: Wen-Xiang
authors.initials: WX
authors.lastname: Ma
authors.forename: Shu-Tao
authors.initials: ST
authors.lastname: Zhang
authors.forename: Shu
authors.initials: S
authors.lastname: Zhang
authors.forename: Peng
authors.initials: P
authors.lastname: Ren
authors.forename: Jiao-Yong
authors.initials: JY
authors.lastname: Li
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D024042:Collagen Type I / Q000378:metabolism; D003288:Contusions / Q000378:metabolism* / Q000473:pathology; D004195:Disease Models, Animal; D049429:Forensic Pathology; D007150:Immunohistochemistry; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D008856:Microscopy, Fluorescence; D018482:Muscle, Skeletal / Q000293:injuries* / Q000378:metabolism* / Q000473:pathology; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014945:Wound Healing
chemical_list: D015415:Biomarkers; D024042:Collagen Type I; D017493:Leukocyte Common Antigens