title: Gold nanoparticle-polymer/biopolymer complexes for protein sensing.
abstract: Nanoparticle-based sensor arrays have been used to distinguish a wide range of biomolecular targets through pattern recognition. Such biosensors require selective receptors that generate a unique response pattern for each analyte. The tunable surface properties of gold nanoparticles make these systems excellent candidates for the recognition process. Likewise, the metallic core makes these particles fluorescence superquenchers, facilitating transduction of the binding event. In this report we analyze the role of gold nanoparticles as receptors in differentiating a diversity of important human proteins, and the role of the polymer/biopolymer fluorescent probes for transducing the binding event. A structure-activity relationship analysis of both the probes and the nanoparticles is presented, providing direction for the engineering of future sensor systems.
authors.affiliation: Department of Chemistry, University of Massachusetts, 710 North Pleasant Street, Amherst, Massachusetts 01003, USA.
authors.forename: Daniel F
authors.initials: DF
authors.lastname: Moyano
authors.forename: Subinoy
authors.initials: S
authors.lastname: Rana
authors.forename: Uwe H F
authors.initials: UH
authors.lastname: Bunz
authors.forename: Vincent M
authors.initials: VM
authors.lastname: Rotello
mesh_terms: D001681:Biological Assay; D001704:Biopolymers / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D002627:Chemistry, Physical; D005456:Fluorescent Dyes; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology; D011485:Protein Binding; D015398:Signal Transduction; D013329:Structure-Activity Relationship
chemical_list: D001704:Biopolymers; D005456:Fluorescent Dyes; D006046:Gold