title: β-adrenoreceptor activation in brain, lung and adipose tissue, measured by microdialysis in pig.
abstract: OBJECTIVE
  The aim of this study is to investigate the effect of local activation of β-adrenoreceptor by Isoprenaline on metabolism in brain, fat and lung measured by microdialysis.
  
  
  METHODS
  We used 8 healthy pigs under general anaesthesia and placed microdialysis catheters in brain, fat, lung and artery. We performed a direct measurement of glucose, lactate, pyruvate and glycerol. The stimulation was performed by one-hour infusion of Isoprenaline, a β-adrenoreceptor agonist.
  
  
  RESULTS
  The infusion of isoprenaline did not affect the glucose in any tissue. The levels of lactate (p=0.008) and pyruvate (p=0.011) decreased significantly in lung after isoprenaline infusion. There was a significant increase in L/P ratio in fat tissue (p=0.001) while no significant changes could be found in brain (p=0.086) and lung (p=0.679). The most pronounced and significant change was observed in glycerol in fat (p<0.001) that increased by 95%.
  
  
  CONCLUSIONS
  The prominent increase in glycerol in fat proved to be a good measure of β-adrenoreceptor activation and a measure of lipolysis. This can be used to online monitor β-adrenoreceptor activation by glycerol measurement in patients.
authors.affiliation: Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden. elham.rostami@ki.se
authors.forename: E
authors.initials: E
authors.lastname: Rostami
authors.forename: M
authors.initials: M
authors.lastname: Bondi
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D008066:Lipolysis; D008168:Lung / Q000378:metabolism*; D017551:Microdialysis / Q000379:methods*; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D013552:Swine
chemical_list: D011943:Receptors, Adrenergic, beta