title: Optimization of an in vitro method to measure the bioaccessibility of polybrominated diphenyl ethers in dust using response surface methodology.
abstract: To more reasonably evaluate human intake of PBDEs via dust ingestion, bioaccessibility should be taken into account. Previously, we developed an in vitro method to determine the bioaccessibility of PBDEs in food. Here, this method was adapted to determine the bioaccessibility of PBDEs in dust and the digestion conditions that influenced the bioaccessibility of PBDEs were optimized. The digestion conditions included the incubation time of dust in the intestinal digestion solution (T), the bile concentration in the intestinal digestion solution (C(bile)), and the ratio of the volume of the intestinal digestion solution to dust (R). The influence of the concentrations of individual PBDE congeners (C(PBDE)) on the bioaccessibility of PBDEs was also investigated. Central composite design was used to build an experimental model and set experimental parameters, and response surface methodology was used to analyze the obtained data. The results showed that the bioaccessibility of PBDEs increased with the increases of C(bile) and R, and was independent of T and C(PBDE). Under the digestion conditions with C(bile) and R being at 4.0-7.0 g/L and 150-250, respectively, the bioaccessibility of PBDEs in the method-dust varied from 39.2% to 72.8%, which were comparable with the bioaccessibility or bioavailability of PBDEs in dust/soil in the literature. Thus, the in vitro method to measure the bioaccessibility of PBDEs in dust was established and validated. Finally, the bioaccessibility of PBDEs in four natural dust samples, which ranged from 36.1% to 43.3%, were determined using the adapted method.
authors.affiliation: State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China. yuyingxin@staff.shu.edu.cn
authors.forename: Yingxin
authors.initials: Y
authors.lastname: Yu
authors.forename: Yuping
authors.initials: Y
authors.lastname: Pang
authors.forename: Xinyu
authors.initials: X
authors.lastname: Zhang
authors.forename: Chen
authors.initials: C
authors.lastname: Li
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Yu
authors.forename: Jiamo
authors.initials: J
authors.lastname: Fu
mesh_terms: D001646:Bile / Q000378:metabolism; D001682:Biological Availability; D004391:Dust / Q000032:analysis*; D055768:Halogenated Diphenyl Ethers / Q000032:analysis* / Q000493:pharmacokinetics*; D006801:Humans; D052638:Particulate Matter / Q000032:analysis* / Q000493:pharmacokinetics*
chemical_list: D004391:Dust; D055768:Halogenated Diphenyl Ethers; D052638:Particulate Matter