title: Developmental regulation and spatiotemporal redistribution of the sumoylation machinery in the rat central nervous system.
abstract: BACKGROUND
  Small Ubiquitin-like MOdifier protein (SUMO) is a key regulator of nuclear functions but little is known regarding the role of the post-translational modification sumoylation outside of the nucleus, particularly in the Central Nervous System (CNS).
  
  
  RESULTS
  Here, we report that the expression levels of SUMO-modified substrates as well as the components of the sumoylation machinery are temporally and spatially regulated in the developing rat brain. Interestingly, while the overall sumoylation is decreasing during brain development, there are progressively more SUMO substrates localized at synapses. This increase is correlated with a differential redistribution of the sumoylation machinery into dendritic spines during neuronal maturation.
  
  
  CONCLUSIONS
  Overall, our data clearly demonstrate that the sumoylation process is developmentally regulated in the brain with high levels of nuclear sumoylation early in the development suggesting a role for this post-translational modification during the synaptogenesis period and a redistribution of the SUMO system towards dendritic spines at a later developmental stage to modulate synaptic protein function.
authors.affiliation: Institut de Pharmacologie Moléculaire et Cellulaire, Centre National de la Recherche Scientifique, Valbonne, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Loriol
authors.forename: Joséphine
authors.initials: J
authors.lastname: Parisot
authors.forename: Gwénola
authors.initials: G
authors.lastname: Poupon
authors.forename: Carole
authors.initials: C
authors.lastname: Gwizdek
authors.forename: Stéphane
authors.initials: S
authors.lastname: Martin
mesh_terms: D000818:Animals; D001921:Brain / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D002478:Cells, Cultured; D002490:Central Nervous System / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D003712:Dendrites / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D055495:Neurogenesis / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D013347:Subcellular Fractions / Q000378:metabolism; D058207:Sumoylation; D013569:Synapses / Q000378:metabolism; D014018:Tissue Distribution; D044763:Ubiquitin-Conjugating Enzymes / Q000737:chemistry / Q000378:metabolism; D000098843:Ubiquitin-Conjugating Enzyme UBC9
chemical_list: D044763:Ubiquitin-Conjugating Enzymes; D000098843:Ubiquitin-Conjugating Enzyme UBC9