title: Detection of murine toxoplasmosis using magnetic bead-based serum peptide profiling by MALDI-TOF MS.
abstract: Establishment of a rapid, highly specific, and accurate method for diagnosis of Toxoplasma gondii infection is essential to control and prevent zoonotic toxoplasmosis. In this study, a novel diagnostic strategy using magnetic bead-based serum peptide profiling by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was developed. The serum peptides (samples I, II, and III) from T. gondii RH strain-infected mice at days 3, 6, and 9 post-infection (p.i.), and healthy mice were enriched by the optimized magnetic bead-based hydrophobic interaction (MB-HIC8). The mass spectrograms were acquired by MALDI-TOF MS, and analyzed by ClinProTools bioinformatics software from Bruker Daltonics. The diagnostic models from T. gondii RH-infected serum peptide profiling of samples I, II, and III were produced by genetic algorithms, and verified by cross-validation. The sample II model could correctly recognize T. gondii RH strain infection in mice at days 3, 6, and 9 p.i. with a sensitivity of 91.1% and a specificity of 96.7%., and also detect T. gondii ME49 strain-infected serum samples at days 3, 6, 9, and 12 p.i. with a sensitivity of 91.7%. The results of the present study suggest that serum peptide profiling by MALDI-TOF MS is a novel potential tool for the clinical diagnosis of acute T. gondii infection.
authors.affiliation: Military Veterinary Institute, Academy of Military Medical Sciences, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun, Jilin Province, People's Republic of China.
authors.forename: Jiping
authors.initials: J
authors.lastname: Li
authors.forename: Hongtao
authors.initials: H
authors.lastname: Jin
authors.forename: Lixia
authors.initials: L
authors.lastname: Li
authors.forename: Limin
authors.initials: L
authors.lastname: Shang
authors.forename: Yongkun
authors.initials: Y
authors.lastname: Zhao
authors.forename: Feng
authors.initials: F
authors.lastname: Wei
authors.forename: Yanjing
authors.initials: Y
authors.lastname: Liu
authors.forename: Quan
authors.initials: Q
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Qian
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D005260:Female; D008280:Magnetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008863:Microspheres; D008954:Models, Biological; D015800:Protozoan Proteins / Q000097:blood; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000662:veterinary*; D014122:Toxoplasma / Q000378:metabolism; D014124:Toxoplasmosis, Animal / Q000097:blood / Q000175:diagnosis*
chemical_list: D015415:Biomarkers; D015800:Protozoan Proteins