title: Recognition of DNA damage by XPC coincides with disruption of the XPC-RAD23 complex.
abstract: The recognition of helix-distorting deoxyribonucleic acid (DNA) lesions by the global genome nucleotide excision repair subpathway is performed by the XPC-RAD23-CEN2 complex. Although it has been established that Rad23 homologs are essential to protect XPC from proteasomal degradation, it is unclear whether RAD23 proteins have a direct role in the recognition of DNA damage. In this paper, we show that the association of XPC with ultraviolet-induced lesions was impaired in the absence of RAD23 proteins. Furthermore, we show that RAD23 proteins rapidly dissociated from XPC upon binding to damaged DNA. Our data suggest that RAD23 proteins facilitate lesion recognition by XPC but do not participate in the downstream DNA repair process.
authors.affiliation: Department of Genetics, Josephine Nefkens Institute, Erasmus Medical Center, 3015 GE Rotterdam, Netherlands.
authors.forename: Steven
authors.initials: S
authors.lastname: Bergink
authors.forename: Wendy
authors.initials: W
authors.lastname: Toussaint
authors.forename: Martijn S
authors.initials: MS
authors.lastname: Luijsterburg
authors.forename: Christoffel
authors.initials: C
authors.lastname: Dinant
authors.forename: Sergey
authors.initials: S
authors.lastname: Alekseev
authors.forename: Jan H J
authors.initials: JH
authors.lastname: Hoeijmakers
authors.forename: Nico P
authors.initials: NP
authors.lastname: Dantuma
authors.forename: Adriaan B
authors.initials: AB
authors.lastname: Houtsmuller
authors.forename: Wim
authors.initials: W
authors.lastname: Vermeulen
mesh_terms: D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D004249:DNA Damage; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D051379:Mice
chemical_list: D004268:DNA-Binding Proteins; C475578:Rad23a protein, mouse; C499250:Rad23b protein, mouse; C086984:XPC protein, human; D004247:DNA