title: Application of mesenchymal stem cells as a vehicle to deliver replication-competent adenovirus for treating malignant glioma.
abstract: Although gene therapy was regarded as a promising approach for glioma treatment, its therapeutic efficacy was often disappointing because of the lack of efficient drug delivery systems. Mesenchymal stem cells(MSCs) have been reported to have a tropism for brain tumors and thus could be used as delivery vehicles for glioma therapy. Therefore, in this study, we attempted to treat glioma by using MSCs as a vehicle for delivering replication-competent adenovirus. We firstly compared the infectivity of type 3, type 5, and type 35 fiber-modified adenoviruses in MSCs. We also determined suitable adenovirus titer in vitro and then used this titer to analyze the ability of MSCs to deliver replication-competent adenovirus into glioma in vivo. Our results indicated that type 35 fiber-modified adenovirus showed higher infectivity than did naked type 3 or type 5 fiber-modified adenovirus. MSCs carrying replication-competent adenovirus significantly inhibited tumor growth in vivo compared with other control groups. In conclusion, MSCs are an effective vehicle that can successfully transport replication-competent adenovirus into glioma, making it a potential therapeutic strategy for treating malignant glioma.
authors.affiliation: Department of Oncology, Affiliated Hospital of Yanbian University, Yanji, Jilin 133000, PR China.
authors.forename: Cui
authors.initials: C
authors.lastname: Hai
authors.forename: Yong-Min
authors.initials: YM
authors.lastname: Jin
authors.forename: Wen-Biao
authors.initials: WB
authors.lastname: Jin
authors.forename: Zhe-Zhu
authors.initials: ZZ
authors.lastname: Han
authors.forename: Mei-Nv
authors.initials: MN
authors.lastname: Cui
authors.forename: Xue-Zhe
authors.initials: XZ
authors.lastname: Piao
authors.forename: Xiong-Hu
authors.initials: XH
authors.lastname: Shen
authors.forename: Song-Nan
authors.initials: SN
authors.lastname: Zhang
authors.forename: Hong-Hua
authors.initials: HH
authors.lastname: Sun
mesh_terms: D000256:Adenoviridae; D000818:Animals; D001932:Brain Neoplasms / Q000473:pathology / Q000628:therapy*; D045744:Cell Line, Tumor; D005822:Genetic Vectors; D005910:Glioma / Q000473:pathology / Q000628:therapy*; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D050130:Oncolytic Virotherapy; D011897:Random Allocation; D014779:Virus Replication; D023041:Xenograft Model Antitumor Assays