title: Performance of anti-deamidated gliadin peptides antibodies in celiac disease diagnosis.
abstract: OBJECTIVE
  To assess the usefulness of anti-deamidated gliadin peptides antibodies (a-DGP), in the diagnostic of celiac disease (CD).
  
  
  METHODS
  One hundred and three untreated CD patients (67 children and 36 adults) and 36 celiac patients under gluten-free diet were studied. Two hundred and seventy-four subjects served as controls (114 healthy blood donors, 80 healthy children and 80 patients with primary biliary cirrhosis). a-DGP (IgG and IgA) and anti-tissue transglutaminase antibodies (AtTG) were detected by enzyme-linked immunosorbent assay (Elisa). Anti-endomysium antibodies (AEA) were detected by indirect immunofluorescence on human umbilical cord.
  
  
  RESULTS
  The sensitivitiy of IgG and IgA a-DGP were 94% and 97% respectively, compared to 96% for AEA and AtTG. The specificity of a-DGP was 93.6% for IgG and 92% for IgA. The specificity of AEA and AtTG were 100%. The frequency of IgG and IgA a-DGP was significantly higher in patients with CD than in control group (94% vs. 4.4%, P<10(-7); 97% vs. 8%, P<10(-7)). The frequency of IgG a-DGP was the same in children and adult (94%). The frequency of IgA a-DGP were similar in children and adults (95.5% vs. 100%).
  
  
  CONCLUSIONS
  Our study shows that a-DGP increases neither the sensitivity nor the specificity of AEA and AtTG.
authors.affiliation: Research unit (03UR/07-02), Faculty of Pharmacy, rue Avicenne, 5000 Monastir, Tunisia.
authors.forename: Wahiba
authors.initials: W
authors.lastname: Sakly
authors.forename: Amani
authors.initials: A
authors.lastname: Mankaï
authors.forename: Azza
authors.initials: A
authors.lastname: Ghdess
authors.forename: Achouak
authors.initials: A
authors.lastname: Achour
authors.forename: Yosra
authors.initials: Y
authors.lastname: Thabet
authors.forename: Ibtissem
authors.initials: I
authors.lastname: Ghedira
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001323:Autoantibodies / Q000097:blood*; D002446:Celiac Disease / Q000097:blood* / Q000175:diagnosis* / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D003641:Deamination; D005260:Female; D005903:Gliadin / Q000276:immunology*; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D010446:Peptide Fragments / Q000276:immunology*; D012189:Retrospective Studies; D055815:Young Adult
chemical_list: D001323:Autoantibodies; D010446:Peptide Fragments; D005903:Gliadin