title: Myostatin gene targeting in cultured China Han ovine myoblast cells.
abstract: Myostatin (MSTN), a member of the transforming growth factor-β superfamily, has been shown to be a negative regulator of myogenesis. Natural mutation in beef cattle causes double-muscling phenotypes. We report an investigation designed to knockout the MSTN gene by gene targeting in ovine myoblast cells. Two promoter-trap targeting vectors MSTN-green fluorescent protein (GFP) and MSTN-neo were constructed and used to transfect foetal and neonatal ovine primary myoblast cells. Both GFP-expressing cells and drug-resistant cells were obtained. Targeted cells expressing GFP were confirmed by polymerase chain reaction (PCR) assay and drug-resistant cells were characterised by PCR and Southern blot after growing into cell clones.
authors.affiliation: 1State Key Laboratory for Agrobiotechnology, College of Biology, China Agricultural University, Yuanming Yuan West Road #2, Haidian District, 100094 Beijing, People's Republic of China.
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: X
authors.initials: X
authors.lastname: Yang
authors.forename: X
authors.initials: X
authors.lastname: An
authors.forename: Y
authors.initials: Y
authors.lastname: Chen