title: [Mechanisms of Chansu Injection in reversing multidrug resistance of HL60/ADM cells].
abstract: OBJECTIVE
  To investigate the mechanisms underlying the effect of Chansu Injection (CHS) in reversing multi-drug resistance (MDR) of HL60/ADM cells.
  
  
  METHODS
  MTT assay was used to investigate the effect of CHS on adramycin (ADM) sensitivity of HL-60/ADM cells. Flow cytometry was used to observe the effect of CHS on the cell cycle of HL60/ADM cell. The expressions of NF-κB, MRP, GST-π, and iNOS were detected by immunocytochemistry.
  
  
  RESULTS
  Treatment with CHS lowered the IC(50) of ADM in HL60/ADM cells from 34.1971 µmol/L to 17.4393 µmol/L, and caused an increase in G(0)/G1 and G(2)/M phase cells with decreased S phase cells. CHS decreased the expressions of MRP mRNA and GST-π and MRP proteins but increased the expressions of iNOS and NF-κB proteins in the cells.
  
  
  CONCLUSIONS
  CHS can partly reverse MDR in HL60/ADM cells possibly by down-regulating MRP and GST-π, up-regulating NF-κB and iNOS, and promoting cell apoptosis, thereby increase ADM sensitivity of HL-60/ADM cells.
authors.affiliation: Laboratory Research Center, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China. xiongxi7104@sina.com
authors.forename: Xin
authors.initials: X
authors.lastname: Xiong
authors.forename: Yuan-da
authors.initials: YD
authors.lastname: Zhou
mesh_terms: D000664:Amphibian Venoms / Q000494:pharmacology; D002018:Bufanolides / Q000494:pharmacology*; D004317:Doxorubicin / Q000494:pharmacology*; D018432:Drug Resistance, Multiple / Q000187:drug effects*; D019008:Drug Resistance, Neoplasm / Q000187:drug effects*; D018922:HL-60 Cells; D006801:Humans
chemical_list: D000664:Amphibian Venoms; D002018:Bufanolides; C095591:chan su; D004317:Doxorubicin