title: Characterization of a novel binding protein for Fortilin/TCTP--component of a defense mechanism against viral infection in Penaeus monodon.
abstract: The Fortilin (also known as TCTP) in Penaeus monodon (PmFortilin) and Fortilin Binding Protein 1 (FBP1) have recently been shown to interact and to offer protection against the widespread White Spot Syndrome Virus infection. However, the mechanism is yet unknown. We investigated this interaction in detail by a number of in silico and in vitro analyses, including prediction of a binding site between PmFortilin/FBP1 and docking simulations. The basis of the modeling analyses was well-conserved PmFortilin orthologs, containing a Ca(2+)-binding domain at residues 76-110 representing a section of the helical domain, the translationally controlled tumor protein signature 1 and 2 (TCTP_1, TCTP_2) at residues 45-55 and 123-145, respectively. We found the pairs Cys59 and Cys76 formed a disulfide bond in the C-terminus of FBP1, which is a common structural feature in many exported proteins and the "x-G-K-K" pattern of the amidation site at the end of the C-terminus. This coincided with our previous work, where we found the "x-P-P-x" patterns of an antiviral peptide also to be located in the C-terminus of FBP1. The combined bioinformatics and in vitro results indicate that FBP1 is a transmembrane protein and FBP1 interact with N-terminal region of PmFortilin.
authors.affiliation: Center for Genomics and Bioinformatics Research, Faculty of Science, Prince of Songkla University, Songkhla, Thailand.
authors.forename: Tanate
authors.initials: T
authors.lastname: Panrat
authors.forename: Patuma
authors.initials: P
authors.lastname: Sinthujaroen
authors.forename: Benjamas
authors.initials: B
authors.lastname: Nupan
authors.forename: Warapond
authors.initials: W
authors.lastname: Wanna
authors.forename: Martti Tapani
authors.initials: MT
authors.lastname: Tammi
authors.forename: Amornrat
authors.initials: A
authors.lastname: Phongdara
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D019295:Computational Biology; D017931:DNA Primers / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D018613:Microscopy, Confocal; D008958:Models, Molecular; D056004:Molecular Dynamics Simulation; D033561:Penaeidae / Q000276:immunology* / Q000821:virology; D011487:Protein Conformation; D000090324:Tumor Protein, Translationally-Controlled 1; D020798:Two-Hybrid System Techniques; D046848:White spot syndrome virus 1 / Q000276:immunology / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D002352:Carrier Proteins; D017931:DNA Primers; D000090324:Tumor Protein, Translationally-Controlled 1; D049452:Green Fluorescent Proteins