title: [Value of cytopathology in endobronchial ultrasound-guided transbronchial needle aspiration].
abstract: OBJECTIVE
  To evaluate the role of cytopathology in endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) for lung tumor diagnosis and staging.
  
  
  METHODS
  Two-hundred consecutive cases of lung tumor with EBUS-TBNA performed during the period from April, 2009 to September, 2010 in Shanghai Cancer Hospital were retrospectively reviewed. The cytologic diagnoses were categorized as non-diagnostic, negative, suspicious and malignant. When available, cell block preparation and immunohistochemistry were performed. On the 22 positive cases diagnosed by on-site evaluation, epidermal growth factor receptor (EGFR) mutation study was carried out.
  
  
  RESULTS
  In the 200 cases of cytology specimens, 122 cases (69.3%) were diagnosed as malignant, 42 cases (23.9%) as benign and 12 cases (6.8%) as suspicious for malignancy. The non-diagnostic rate was 12.0% (24/200). Amongst the 200 cases studied, 140 cases (70.0%) had histologic correlation available (via core biopsy, mediastinoscopic biopsy or surgical excision). The sensitivity and specificity of EBUS-TBNA cytologic diagnoses were 94.4% and 100%, when using histopathologic findings and clinical follow-up data as gold standard. The cell block preparation and immunohistochemistry were useful in subtyping and diagnosis of extrathoracic malignancy. EGFR mutations were detected in 8 cytology samples (36.4%).
  
  
  CONCLUSIONS
  EBUS-TBNA is a sensitive and specific tool for diagnosis and staging of lung cancer. The cytology samples can be used for further ancillary investigations including cell block preparation, immunohistochemistry and molecular studies.
authors.affiliation: Department of Pathology, Fudan University Shanghai Cancer Center and Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.forename: Bo
authors.initials: B
authors.lastname: Ping
authors.forename: Long-fu
authors.initials: LF
authors.lastname: Wang
authors.forename: Li-qing
authors.initials: LQ
authors.lastname: Feng
authors.forename: Wei-ping
authors.initials: WP
authors.lastname: Xu
authors.forename: Jia-wen
authors.initials: JW
authors.lastname: Wu
authors.forename: Wen-tao
authors.initials: WT
authors.lastname: Yang
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: Zhou
authors.forename: Xu
authors.initials: X
authors.lastname: Cai
authors.forename: Hong
authors.initials: H
authors.lastname: Hu
authors.forename: Hai-quan
authors.initials: HQ
authors.lastname: Chen
authors.forename: Lei
authors.initials: L
authors.lastname: Shen
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001980:Bronchi; D018288:Carcinoma, Small Cell / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D061765:Endoscopic Ultrasound-Guided Fine Needle Aspiration / Q000379:methods*; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism; D005091:Exons; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D008481:Mediastinoscopy; D008875:Middle Aged; D009154:Mutation; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: C512478:EGFR protein, human; D066246:ErbB Receptors