title: Profile of eicosanoids in breath condensate in asthma and COPD.
abstract: The collection of exhaled breath condensates (EBC) is a noninvasive method for obtaining samples from the lungs. Eicosanoids are lipid mediators implicated in the asthmatic inflammatory response. The objective of our study was to investigate whether the profile of eicosanoid lipid mediators in EBC can characterize the inflammation in asthma and chronic obstructive pulmonary disease (COPD). EBC samples were collected from 22 healthy controls (C), 25 mild intermittent asthmatics (MIA), 20 with moderate to severe asthma (MSA) and 20 with moderate to severe COPD. EBC samples were analyzed by unique tandem mass spectrometry that allows the quantification of up to 25 eicosanoid mediators simultaneously. No differences were found between MIA and C. Subjects with MSA and COPD had higher levels of 6-keto, PGE2, LTB4, 11-12 EET and AA, while lower levels of LXA4, 11DHyTxB2, 11HETE and 8,9EET, when compared to MSA and C (p < 0.05). Our study shows that the analysis of EBC through mass spectrometry is mixed and has a similar response in MSA and COPD when compared to MIA and controls.
authors.affiliation: Potificia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil. Leandro.fritscher@pucrs.br
authors.forename: Leandro Genehr
authors.initials: LG
authors.lastname: Fritscher
authors.forename: Martin
authors.initials: M
authors.lastname: Post
authors.forename: Marcelo Tadday
authors.initials: MT
authors.lastname: Rodrigues
authors.forename: Frances
authors.initials: F
authors.lastname: Silverman
authors.forename: Mayer
authors.initials: M
authors.lastname: Balter
authors.forename: Kenneth R
authors.initials: KR
authors.lastname: Chapman
authors.forename: Noe
authors.initials: N
authors.lastname: Zamel
mesh_terms: D000328:Adult; D000368:Aged; D001249:Asthma / Q000175:diagnosis* / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis; D001944:Breath Tests / Q000379:methods*; D015777:Eicosanoids / Q000032:analysis*; D045853:Exhalation; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D029424:Pulmonary Disease, Chronic Obstructive / Q000175:diagnosis* / Q000378:metabolism; D012720:Severity of Illness Index
chemical_list: D015415:Biomarkers; D015777:Eicosanoids