title: Study of antibacterial activity by capillary electrophoresis using multiple UV detection points.
abstract: A new methodology for an antibacterial assay based on capillary electrophoresis with multiple UV detection points has been proposed. The possible antibacterial activity of cationic molecules on bacteria (Gram-positive and Gram-negative) is studied by detecting the bacteria before, during, and after their meeting with the cationic antibacterial compound. For that, a UV area imaging detector having two loops and three detection windows was used with a 95 cm ×100 μm i.d. capillary. In the antibacterial assay, the bacteria (negatively charged) and the cationic molecules were injected separately from each end of the capillary. The bacteria were mobilized by anionic ITP mode while cationic molecules migrate in the opposite direction under conditions close to CZE. The cationic molecules were injected into the capillary as a broad band (injected volume about 16% of the volume of the capillary) to prevent dilution of the sample during the electrophoretic process. Bacteriolytic activity, as well as strong interactions between the small antibacterial molecules and the bacteria, can be investigated within a few minutes. The assay was used to study the antibacterial activity of dendrigraft poly-L-lysines on Micrococcus luteus and Erwinia carotovora. Because dendrigraft poly-L-lysines are nonimmunogenic and have low toxicity, this new class of dendritic biomacromolecules is very promising for antibacterial applications.
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM, UMR 5247 CNRS-Université de Montpellier 1-Université de Montpellier 2), Place Eugène Bataillon, case courrier 1706, 34095 Montpellier Cedex 5, France.
authors.forename: Farid
authors.initials: F
authors.lastname: Oukacine
authors.forename: Bernard
authors.initials: B
authors.lastname: Romestand
authors.forename: David M
authors.initials: DM
authors.lastname: Goodall
authors.forename: Gladys
authors.initials: G
authors.lastname: Massiera
authors.forename: Laurent
authors.initials: L
authors.lastname: Garrelly
authors.forename: Hervé
authors.initials: H
authors.lastname: Cottet
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001565:Benzoates / Q000737:chemistry; D001596:Benzylamines / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D016982:Micrococcus luteus / Q000737:chemistry / Q000187:drug effects; D016973:Pectobacterium carotovorum / Q000737:chemistry / Q000187:drug effects; D011107:Polylysine / Q000737:chemistry / Q000494:pharmacology; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents; D001565:Benzoates; D001596:Benzylamines; D011107:Polylysine; C030796:benzylamine