title: Combination therapy with BMP-2 and BMSCs enhances bone healing efficacy of PCL scaffold fabricated using the 3D plotting system in a large segmental defect model.
abstract: The three-dimensional (3D) plotting system is a rapidly-developing scaffold fabrication method for bone tissue engineering. It yields a highly porous and inter-connective structure without the use of cytotoxic solvents. However, the therapeutic effects of a scaffold fabricated using the 3D plotting system in a large segmental defect model have not yet been demonstrated. We have tested two hypotheses: whether the bone healing efficacy of scaffold fabricated using the 3D plotting system would be enhanced by bone marrow-derived mesenchymal stem cell (BMSC) transplantation; and whether the combination of bone morphogenetic protein-2 (BMP-2) administration and BMSC transplantation onto the scaffold would act synergistically to enhance bone regeneration in a large segmental defect model. The use of the combined therapy did increase bone regeneration further as compared to that with monotherapy in large segmental bone defects.
authors.affiliation: Department of Orthopaedic Surgery, Korea University, Ansan Hospital, Ansan, Korea. seraphsun@hanyang.ac.kr
authors.forename: Sun-Woong
authors.initials: SW
authors.lastname: Kang
authors.forename: Ji-Hoon
authors.initials: JH
authors.lastname: Bae
authors.forename: Su-A
authors.initials: SA
authors.lastname: Park
authors.forename: Wan-Doo
authors.initials: WD
authors.lastname: Kim
authors.forename: Mi-Su
authors.initials: MS
authors.lastname: Park
authors.forename: You-Jin
authors.initials: YJ
authors.lastname: Ko
authors.forename: Hyon-Seok
authors.initials: HS
authors.lastname: Jang
authors.forename: Jung-Ho
authors.initials: JH
authors.lastname: Park
mesh_terms: D000818:Animals; D001853:Bone Marrow; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism*; D001861:Bone Regeneration; D001842:Bone and Bones / Q000502:physiology; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D011091:Polyesters; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D055396:Bone Morphogenetic Protein 2; D011091:Polyesters; C016240:polycaprolactone