title: [Development of a xMAP liquid chip assay for the rapid identification of 7 common foodborne pathogens and its application].
abstract: OBJECTIVE
  To develop an assay for the simultaneous detection of 7 common foodborne pathogens with xMAP liquid chip in a single-tube reaction.
  
  
  METHODS
  Seven specific primers and probes were designed and synthesized based on the target gene sequences from GenBank. Target bacterial sequences were amplified by asymmetric PCR. The biotinylated products were hybridized to seven probe beads in a multiplex reaction and analyzed by using streptavidin conjugated to a fluorescent reporter molecule. The developed liquid chip xMAP assay was used to test 140 strains of bacteria and then 56 food samples.
  
  
  RESULTS
  No cross-reaction and false signals were observed. The detection limit was 1 - 100 pg and 10(5) - 10(6) cfu/ml. The results tested by xMAP were in accordance with the traditional culture method.
  
  
  CONCLUSIONS
  The processing of xMAP liquid chip assay, including DNA preparation and sample detection, could be finished within 3.5 hours and could be applied to the classification and identification of foodborne pathogens in the food safety monitoring.
authors.affiliation: Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China.
authors.forename: Dongyue
authors.initials: D
authors.lastname: Lü
authors.forename: Xiaolu
authors.initials: X
authors.lastname: Shi
authors.forename: Miaoling
authors.initials: M
authors.lastname: Chen
authors.forename: Piugfang
authors.initials: P
authors.lastname: Wu
authors.forename: Lianhua
authors.initials: L
authors.lastname: He
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Li
authors.forename: Yiman
authors.initials: Y
authors.lastname: Lin
authors.forename: Yaqun
authors.initials: Y
authors.lastname: Qiu
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Hu
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification; D001431:Bacteriological Techniques; D019453:Escherichia coli O157 / Q000235:genetics / Q000302:isolation & purification; D005506:Food Contamination; D005516:Food Microbiology / Q000379:methods*; D008863:Microspheres; D021141:Nucleic Acid Amplification Techniques; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods; D012475:Salmonella / Q000235:genetics / Q000302:isolation & purification*; D013211:Staphylococcus aureus / Q000235:genetics / Q000302:isolation & purification*; D014736:Vibrio parahaemolyticus / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D015345:Oligonucleotide Probes