title: Acetobacter pasteurianus strain AB0220: cultivability and phenotypic stability over 9 years of preservation.
abstract: Acetobacter species are members of the α-subclass of Proteobacteria, which harbors a large number of bacteria recalcitrant to cultivation. Strain AB0220 was isolated from a superficial acetification system and preserved for 9 years by short and long time methods. Under short time preservation it was estimated that 540.54 number of generations occurred, whereas in long time preservation conditions the number of generations was 17.40. Ethanol oxidation to acetic acid was stable and confirmed, as well as acetate assimilation during long time preservation. Cultivability checks showed persistence of phenotypic traits (growth on ethanol and methanol, growth on different carbon sources and cellulose production) over the extended preservation time. 16S rRNA gene sequences analysis showed 100 % of similarity with A. pasteurianus (Accession number GQ240636). Stability of subcultures related to the culture age and subcultures frequency, tested by ERIC/PCR, confirmed the suitability of long term preservation at least over a period of 9 years.
authors.affiliation: Department of Agricultural and Food Science, University of Modena and Reggio Emilia, Reggio Emilia, Italy. maria.gullo@unimore.it
authors.forename: Maria
authors.initials: M
authors.lastname: Gullo
authors.forename: Dhouha
authors.initials: D
authors.lastname: Mamlouk
authors.forename: Luciana
authors.initials: L
authors.lastname: De Vero
authors.forename: Paolo
authors.initials: P
authors.lastname: Giudici
mesh_terms: D019342:Acetic Acid / Q000378:metabolism; D000091:Acetobacter / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D002244:Carbon / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D000431:Ethanol / Q000378:metabolism; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010641:Phenotype; D011309:Preservation, Biological; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D000431:Ethanol; D002244:Carbon; D019342:Acetic Acid