title: The effects of a low vitamin E diet on dichloroacetate- and trichloroacetate-induced oxidative stress in the livers of mice.
abstract: Groups of mice were fed either a standard (Std) diet or a diet not supplemented with vitamin E (Low-E) and were divided into three subgroups that were treated subchronically by gavage, with water (control), dichloroacetate (DCA), or trichloroacetate (TCA). The livers of the animals were assayed for various biomarkers of oxidative stress (OS), antioxidant enzyme activities, and total glutathione (GSH). In general, livers from the low-E diet group expressed lower levels of biomarkers of OS associated with greater increases in various antioxidant enzymes activities and GSH when compared with the corresponding treatments in the Std diet group. These results suggest that vitamin E supplementation to the diet, while essential to maintain certain body functions, can compromise the effectiveness of the hepatic antioxidant enzymes and GSH resulting in an increase in DCA- and TCA-induced OS and a possible increase in the compounds-induced hepatotoxic/hepatocarcinogenic effects in mice.
authors.affiliation: College of Pharmacy and Pharmaceutical Sciences, The University of Toledo, Health Science Campus, Toledo, OH 43614-2598, USA.
authors.forename: Jacquelyn
authors.initials: J
authors.lastname: Cearfoss
authors.forename: Ezdihar
authors.initials: E
authors.lastname: Hassoun
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002374:Catalase / Q000378:metabolism; D053904:DNA Breaks, Single-Stranded; D003999:Dichloroacetic Acid / Q000494:pharmacology*; D004032:Diet; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D014238:Trichloroacetic Acid / Q000494:pharmacology*; D014810:Vitamin E / Q000008:administration & dosage*; D014811:Vitamin E Deficiency / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D013481:Superoxides; D014810:Vitamin E; D014238:Trichloroacetic Acid; D003999:Dichloroacetic Acid; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione