title: Assays of dioxins and dioxin-like compounds in actually contaminated soils using transgenic tobacco plants carrying a recombinant mouse aryl hydrocarbon receptor-mediated β-glucuronidase reporter gene expression system.
abstract: The transgenic tobacco plant XD4V-26 carrying the recombinant mouse aryl hydrocarbon receptor XD4V-mediated β-glucuronidase (GUS) reporter gene expression system was used for assay of dioxins and dioxin-like compounds consisting of polychlorodibenzo-p-dioxins, polychlorinated dibenzofurans, and coplanar polychlorinated biphenyls (Co-PCBs) in actually contaminated soils. The transgenic tobacco plant XD4V-26 showed a significant dose-dependent induced GUS activity when cultured on MS medium containing PCB126 [toxic equivalency factor (TEF) = 0.1]. In contrast, PCB169 and PCB180, which have 0.03 of TEF and unassigned TEF values, respectively, did not significantly induce GUS activity under the same conditions as with PCB126. When the tobacco plants were cultivated for up to 5 weeks on actually contaminated soils with dioxins and dioxin-like compounds collected from the periphery of an incinerator used for disposal of life and industrial wastes, GUS activity in the leaves was dose-dependently increased. The plants clearly detected 360 pg-TEQ g(-1) of dioxins and dioxin-like compounds in this assay. There was a positive correlation between GUS activity and TEQ value of dioxins and dioxin-like compounds in the plants. This assay does not require any extraction and purification processes for the actually contaminated soil samples.
authors.affiliation: Research Center for Environmental Genomics, Kobe University, Kobe, Hyogo, Japan. hideo.ohkawa@gmail.com
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Inui
authors.forename: Keiko
authors.initials: K
authors.lastname: Gion
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Utani
authors.forename: Taketo
authors.initials: T
authors.lastname: Wakai
authors.forename: Susumu
authors.initials: S
authors.lastname: Kodama
authors.forename: Heesoo
authors.initials: H
authors.lastname: Eun
authors.forename: Yun-Seok
authors.initials: YS
authors.lastname: Kim
authors.forename: Hideo
authors.initials: H
authors.lastname: Ohkawa
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics*; D004147:Dioxins / Q000032:analysis*; D004784:Environmental Monitoring / Q000379:methods*; D015870:Gene Expression; D017930:Genes, Reporter; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D051379:Mice; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000378:metabolism; D018336:Receptors, Aryl Hydrocarbon / Q000235:genetics*; D012987:Soil / Q000032:analysis; D012989:Soil Pollutants / Q000032:analysis*; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D004147:Dioxins; D005966:Glucuronidase; D018336:Receptors, Aryl Hydrocarbon; D012987:Soil; D012989:Soil Pollutants; C484722:Ahr protein, mouse