title: Immunomodulatory effects of human amniotic membrane-derived mesenchymal stem cells.
abstract: Human amniotic membrane-derived mesenchymal stem cells (hAM-MSCs) are capable of differentiating into several lineages and possess immunomodulatory properties. In this study, we investigated the soluble factor-mediated immunomodulatory effects of hAM-MSCs. Mitogen-induced peripheral blood mononuclear cell (PBMC) proliferation was suppressed by hAM-MSCs in a dose-dependent manner as well as hAM-MSC culture supernatant. Moreover, interferon-gamma and interleukin (IL)-17 production significantly decreased from PBMC, whereas IL-10 from PBMCs and transforming growth factor beta (TGF-β) production from hAM-MSCs significantly increased in co-cultures of hAM-MSCs and PBMCs. Production of several MSC factors, including hepatocyte growth factor (HGF), TGF-β, prostaglandin E2 (PGE2), and indoleamine 2, 3 dioxygenase (IDO), increased significantly in hAM-MSCs co-cultured with PBMCs. These results indicate that the immunomodulatory effects of hAM-MSCs may be associated with soluble factors (TGF-β, HGF, PGE2, and IDO), suggesting that hAM-MSCs may have potential clinical use in regenerative medicine.
authors.affiliation: Department of Microbiology, College of Veterinary Medicine, and BK 21 Program for Veterinary Science, Seoul National University, Seoul 151-742, Korea.
authors.forename: Jung Won
authors.initials: JW
authors.lastname: Kang
authors.forename: Hye Cheong
authors.initials: HC
authors.lastname: Koo
authors.forename: Sun Young
authors.initials: SY
authors.lastname: Hwang
authors.forename: Sung Keun
authors.initials: SK
authors.lastname: Kang
authors.forename: Jeong Chan
authors.initials: JC
authors.lastname: Ra
authors.forename: Moon Han
authors.initials: MH
authors.lastname: Lee
authors.forename: Yong Ho
authors.initials: YH
authors.lastname: Park
mesh_terms: D000650:Amnion / Q000166:cytology / Q000276:immunology*; D002454:Cell Differentiation / Q000276:immunology; D018920:Coculture Techniques; D015232:Dinoprostone / Q000235:genetics / Q000276:immunology; D005260:Female; D017228:Hepatocyte Growth Factor / Q000235:genetics / Q000276:immunology; D006801:Humans; D007155:Immunologic Factors / Q000276:immunology*; D016130:Immunophenotyping; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase / Q000235:genetics / Q000276:immunology; D007371:Interferon-gamma / Q000276:immunology; D016753:Interleukin-10 / Q000032:analysis / Q000276:immunology; D020381:Interleukin-17 / Q000032:analysis / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000276:immunology*; D011247:Pregnancy; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D044968:Regenerative Medicine / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000276:immunology
chemical_list: D007155:Immunologic Factors; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D020381:Interleukin-17; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D016753:Interleukin-10; D017228:Hepatocyte Growth Factor; D007371:Interferon-gamma; D015232:Dinoprostone