title: Waveguide-mode sensors as aptasensors.
abstract: Aptamers are artificial nucleic acid ligands that can be generated by in vitro selection through partition and amplification. Aptamers can be generated against a wide range of biomolecules through the formation of versatile stem-loop structures. Because aptamers are potential substitutes for antibodies and drugs, the development of an aptamer-based sensor (aptasensor) is mandatory for diagnosis. We previously reported that waveguide-mode sensors are useful in the analysis of a wide range of biomolecular interactions, including aptamers. The advantages of the waveguide-mode sensor that we developed include physical and chemical stability and that higher sensitivity can be achieved with ease by perforating the waveguide layer or using colored materials such as dyes or metal nanoparticles as labels. Herein, we provide an overview of the strategies and applications for aptamer-based analyses using waveguide-mode sensors.
authors.affiliation: Electronics and Photonics Research Institute, National Institute of Advanced Industrial Science and Technology, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8562, Japan. gopi-subashchandrabose@aist.go.jp
authors.forename: Subash C B
authors.initials: SC
authors.lastname: Gopinath
authors.forename: Koichi
authors.initials: K
authors.lastname: Awazu
authors.forename: Makoto
authors.initials: M
authors.lastname: Fujimaki
mesh_terms: D052157:Aptamers, Nucleotide / Q000032:analysis* / Q000235:genetics*; D015374:Biosensing Techniques; D004867:Equipment Design; D019544:Equipment Failure Analysis; D055096:Optical Devices; D012031:Refractometry / Q000295:instrumentation*; D020349:Surface Plasmon Resonance / Q000295:instrumentation*; D014159:Transducers
chemical_list: D052157:Aptamers, Nucleotide