title: Analysis of bacterial community in bulking sludge using culture-dependent and -independent approaches.
abstract: The bacterial community of a bulking sludge from a municipal wastewater treatment plant with anoxic-anaerobic-oxic process was investigated by combination of cultivation and 16S rRNA gene clone library analysis for understanding the causes of bulking. A total of 28 species were obtained from 63 isolates collected from six culture media. The most cultivable species belonged to gamma-Proteobacteria including Klebsiella sp., Pseudomonas sp., Aeromonas sp. and Acinetobacter sp. Further analysis of these strains by repetitive sequence based on polymerase chain reaction (rep-PCR) technology showed that rep-PCR yielded discriminatory banding patterns within the same genus using REP and BOX primer sets. While the culture-independent assessment revealed that beta-Proteobacteria was the dominant group in the bulking sample. Sequence analysis revealed that the highest proportion (14.7%) of operational taxonomic units was 98% similar to Candidatus Accumulibacter phosphatis, which is used to remove phosphorous from wastewater. Our results indicated that combining different approaches can produce complementary information, thus generate a more accurate view of microbial community in bulking sludge.
authors.affiliation: Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. jindecai19841019@yahoo.com.cn
authors.forename: Decai
authors.initials: D
authors.lastname: Jin
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Bai
authors.forename: Xinxin
authors.initials: X
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Peng
authors.forename: Rong
authors.initials: R
authors.lastname: Qi
authors.forename: Zhisheng
authors.initials: Z
authors.lastname: Yu
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Zhuang
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000254:growth & development; D044822:Biodiversity; D046508:Culture Techniques; D016172:DNA Fingerprinting; D015723:Gene Library; D005798:Genes, Bacterial; D020459:Genes, rRNA; D059013:Microbial Consortia; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000382:microbiology*
chemical_list: D012336:RNA, Ribosomal, 16S; D012722:Sewage