title: Analysis of cartilage oligomeric matrix protein and matrix metalloproteinase-9 in cerebrospinal fluid of miniature dachshund with intervertebral disc herniation.
abstract: We evaluated whether the cerebrospinal fluid (CSF) concentration of cartilage oligomeric matrix protein (COMP) is related to disease severity, prognosis and matrix metalloproteinase (MMP)-9 activity of the CSF in miniature dachshund with intervertebral disc herniation. Samples were obtained from 23 patients and 6 normal dogs, and all patients received hemilaminectomy. Twenty dogs recovered successfully and 3 of 11 dogs without deep nociception had MMP-9 activity in the CSF and an unsuccessful outcome. The COMP levels from patients were significantly higher than those from normal dogs. MMP-9 activity and neurological severity were not related to the COMP levels. However, the COMP levels from 3 unsuccessful cases that had MMP-9 activity were significantly lower than those from all recovered cases and/or successful cases without deep nociception. Concerning severe cases, increased proteolytic activity might affect the COMP concentration and prognosis due to MMP-9 associated deleterious effects.
authors.affiliation: Laboratory of Veterinary Surgery, Department of Veterinary Clinical Science, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.
authors.forename: Shinichi
authors.initials: S
authors.lastname: Nagano
authors.forename: Makoto
authors.initials: M
authors.lastname: Fujiki
authors.forename: Satoshi
authors.initials: S
authors.lastname: Tokunaga
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Misumi
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000134:cerebrospinal fluid* / Q000378:metabolism / Q000601:surgery; D004285:Dogs; D016326:Extracellular Matrix Proteins / Q000134:cerebrospinal fluid* / Q000235:genetics / Q000378:metabolism; D005260:Female; D006023:Glycoproteins / Q000134:cerebrospinal fluid* / Q000235:genetics / Q000378:metabolism; D055959:Intervertebral Disc Degeneration / Q000134:cerebrospinal fluid / Q000601:surgery / Q000662:veterinary*; D008297:Male; D064235:Matrilin Proteins; D020780:Matrix Metalloproteinase 9 / Q000134:cerebrospinal fluid* / Q000235:genetics / Q000378:metabolism
chemical_list: D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D064235:Matrilin Proteins; D020780:Matrix Metalloproteinase 9