title: Influence of the plant extract complex "AdMax" on global gene expression levels in cultured human fibroblasts.
abstract: Ethanol/water extracts from roots of Leuzea carthamoides Iljin, Rhodiola rosea L., Eleutherococcus senticosus Maxim, and from dry berries of Schizandra chinensis Baill. are known as adaptogenic remedies, which enhance physical endurance, counteract fatigue and restore suppressed immunity. Molecular mechanisms underlying effects of the extracts are poorly understood. In this study, a combination of these four extracts called AdMax™ (Nulab, Inc., Florida) was examined for its ability to influence gene expression levels in cultured human fibroblasts in vitro with the help of whole-genome Affymetrix oligonucleotide microarrays. We showed that AdMax treatment results in significant changes (at least 2 fold, p <. 05) in expression of 67 genes that are involved in metabolism of protein, nucleic acids, lipid and carbohydrates, in regulation of transcription, protein and ion transport, response to stimulus and stress. Enhancing expression of the PANK2 gene is of special interest in connection with AdMax ability to enhance physical endurance and counteract fatigue. PANK2 encodes a mitochondrial enzyme pantothenate kinase 2, which provides coenzyme A biosynthesis and thereby plays crucial role in energy metabolism. Partial deficiency of PANK2 gene activity leads to pantothenate kinase-associated neurodegeneration. In this connection potential therapeutic use of AdMax in patients with neurodegenerative diseases is discussed.
authors.affiliation: Genext Research, Inc., Clearwater, Florida 33765, USA. antoshechkin@earthlink.net
authors.forename: Anatoly
authors.initials: A
authors.lastname: Antoshechkin
authors.forename: Jose
authors.initials: J
authors.lastname: Olalde
authors.forename: Marina
authors.initials: M
authors.lastname: Antoshechkina
authors.forename: Vladimir
authors.initials: V
authors.lastname: Briuzgin
authors.forename: Leonid
authors.initials: L
authors.lastname: Platinskiy
mesh_terms: D001692:Biological Transport / Q000235:genetics; D002478:Cells, Cultured; D003065:Coenzyme A / Q000096:biosynthesis; D004338:Drug Combinations; D028001:Eleutherococcus; D004734:Energy Metabolism / Q000187:drug effects* / Q000235:genetics; D005221:Fatigue / Q000517:prevention & control; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000235:genetics; D006801:Humans; D007109:Immunity / Q000187:drug effects; D043843:Leuzea; D019684:Magnoliopsida; D046228:Microarray Analysis; D019636:Neurodegenerative Diseases / Q000188:drug therapy / Q000235:genetics; D018696:Neuroprotective Agents / Q000494:pharmacology / Q000627:therapeutic use; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics*; D010807:Physical Endurance / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D018514:Plant Structures; D029771:Rhodiola; D032141:Schisandra; D013312:Stress, Physiological / Q000187:drug effects* / Q000235:genetics
chemical_list: D004338:Drug Combinations; D018696:Neuroprotective Agents; D010936:Plant Extracts; D017853:Phosphotransferases (Alcohol Group Acceptor); C023088:pantothenate kinase; D003065:Coenzyme A