title: [Evaluation of commercial systems VITEK 2 and API 20NE for identification of Burkholderia cepacia complex bacteria from clinical samples].
abstract: Species belonging to the Burkholderia cepacia complex (BCC) are capable of causing chronic respiratory tract infections in patients suffering from cystic fibrosis as well as in immunocompromised individuals. Most of these species are highly resistant to antibiotic therapy, generating the need for their rapid and accurate detection for the proper treatment and clinical management of these patients. In this work, the polymerase chain reaction (PCR) technique based on the amplification of the recA gene (PCR-recA) was applied for an accurate identification of bacteria belonging to the BCC. Sensitivity (S) and specificity (E) of two biochemically-based commercial automated systems, API 20NE and VITEK 2 (bioMérieux®), and of the most representative biochemical manual tests for the identification of the Burkholderia cepacia complex were herein evaluated. The commercial systems VITEK 2 and API 20NE showed the following sensitivity and specificity vaues for identification to the species level, S: 71.1 %, E: 100 %, S: 69.7 %, E: 90.2 %, respectively. More complex results were observed for phenotypic manual tests, since BCC bacteria can undergo selective pressure to survive in chronic patients causing the loss of their typical phenotypic characteristics. The PCR-recA technique was easy to implement even in medium-complexity clinical diagnostic laboratories.
authors.affiliation: Sala de Microbiología, HIAEP Sor María Ludovica, La Plata.
authors.forename: Sebastián
authors.initials: S
authors.lastname: Oderiz
authors.forename: María J
authors.initials: MJ
authors.lastname: Palau
authors.forename: Paula
authors.initials: P
authors.lastname: Del Palacio
authors.forename: Miriam C
authors.initials: MC
authors.lastname: Lewis
authors.forename: Marisa P
authors.initials: MP
authors.lastname: Bettiol
authors.forename: Pablo
authors.initials: P
authors.lastname: Martina
authors.forename: Alejandra
authors.initials: A
authors.lastname: Bosch
authors.forename: Osvaldo M
authors.initials: OM
authors.lastname: Yantorno
authors.forename: Blanca M
authors.initials: BM
authors.lastname: Gatti
mesh_terms: D001331:Automation; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques / Q000379:methods*; D019121:Burkholderia Infections / Q000175:diagnosis / Q000209:etiology / Q000382:microbiology*; D042602:Burkholderia cepacia complex / Q000302:isolation & purification*; D003124:Colorimetry / Q000379:methods; D003550:Cystic Fibrosis / Q000150:complications; D004269:DNA, Bacterial / Q000235:genetics; D004198:Disease Susceptibility; D005798:Genes, Bacterial; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D011933:Reagent Kits, Diagnostic; D011938:Rec A Recombinases / Q000235:genetics; D012015:Reference Standards; D015203:Reproducibility of Results; D012141:Respiratory Tract Infections / Q000175:diagnosis / Q000209:etiology / Q000382:microbiology*; D012680:Sensitivity and Specificity; D012984:Software
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic; D011938:Rec A Recombinases