title: Copolythiophene-derived colorimetric and fluorometric sensor for visually supersensitive determination of lipopolysaccharide.
abstract: 3-Phenylthiophene-based water-soluble copolythiophenes (CPT1) were designed for colorimetric and fluorometric detection of lipopolysaccharide (LPS). The sensor (CPT1-C) shows a high selectivity to LPS in the presence of other negatively charged bioanalytes as well an extreme sensitivity with the detection limit at picomolar level, which is the lowest ever achieved among all synthetic LPS sensors available thus far. Significantly, the sensing interaction can be apparently observed by the naked eyes, which presents a great advantage for its practical applications. The appealing performance of sensor was demonstrated to originate from the multiple electrostatic and hydrophobic cooperative interactions, synergetic with signal amplification via the conformational change of the 3-phenylthiophene-based copolymer main chain. As a straightforward application, CPT1-C is capable of rapidly discriminating the Gram-negative bacteria (with LPS in the membrane) from Gram-positive bacteria (without LPS).
authors.affiliation: Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190, People's Republic of China.
authors.forename: Minhuan
authors.initials: M
authors.lastname: Lan
authors.forename: Jiasheng
authors.initials: J
authors.lastname: Wu
authors.forename: Weimin
authors.initials: W
authors.lastname: Liu
authors.forename: Wenjun
authors.initials: W
authors.lastname: Zhang
authors.forename: Jiechao
authors.initials: J
authors.lastname: Ge
authors.forename: Hongyan
authors.initials: H
authors.lastname: Zhang
authors.forename: Jiayu
authors.initials: J
authors.lastname: Sun
authors.forename: Wenwen
authors.initials: W
authors.lastname: Zhao
authors.forename: Pengfei
authors.initials: P
authors.lastname: Wang
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000379:methods*; D003124:Colorimetry / Q000379:methods; D005470:Fluorometry / Q000379:methods; D006090:Gram-Negative Bacteria / Q000302:isolation & purification; D006094:Gram-Positive Bacteria / Q000302:isolation & purification; D057230:Limit of Detection; D008070:Lipopolysaccharides / Q000032:analysis*; D011108:Polymers; D013876:Thiophenes
chemical_list: D008070:Lipopolysaccharides; D011108:Polymers; D013876:Thiophenes; C066730:polythiophene