title: Lack of genomic imprinting of DNA primase, polypeptide 2 (PRIM2) in human term placenta and white blood cells.
abstract: PRIM2, encoding a subunit of primase involved in DNA replication and transcription, is expressed in the placenta and is crucial for mammalian development and growth. Its role in placental function is not well understood. Recently, PRIM2 was reported as imprinted in human white blood cells (WBC). We report here our failure to confirm imprinting of the PRIM2 locus in human placenta or WBC. The discordance between our results and those of others are likely due to an incorrectly annotated PRIM2 pseudogene found in the human genome database.
authors.affiliation: Center for Comparative Medicine and Translational Research, North Carolina State University, Raleigh, NC, USA.
authors.forename: Jaewook
authors.initials: J
authors.lastname: Chung
authors.forename: Shengdar
authors.initials: S
authors.lastname: Tsai
authors.forename: Andra H
authors.initials: AH
authors.lastname: James
authors.forename: Betty H
authors.initials: BH
authors.lastname: Thames
authors.forename: Stephanie
authors.initials: S
authors.lastname: Shytle
authors.forename: Jorge A
authors.initials: JA
authors.lastname: Piedrahita
mesh_terms: D000483:Alleles; D019915:DNA Primase / Q000235:genetics / Q000378:metabolism*; D004261:DNA Replication; D030561:Databases, Nucleic Acid; D005260:Female; D005787:Gene Frequency; D056426:Genetic Loci; D015894:Genome, Human; D018392:Genomic Imprinting; D006579:Heterozygote; D006801:Humans; D007962:Leukocytes / Q000166:cytology / Q000201:enzymology*; D058977:Molecular Sequence Annotation; D010920:Placenta / Q000166:cytology / Q000201:enzymology*; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D011544:Pseudogenes; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger; D019915:DNA Primase; C525356:PRIM2 protein, human