title: Characterization of a novel Y2K-type dehydrin VrDhn1 from Vigna radiata.
abstract: A novel dehydrin gene (VrDhn1) was isolated from an embryo cDNA library of Vigna radiata (L.) Wilczek (mungbean) variety VC1973A. The intronless VrDhn1 gene encodes a protein belonging to the Y(2)K-type dehydrin family. VrDhn1 protein accumulated in embryos and cotyledons during seed maturation and disappeared 2 days after seed imbibition (DAI). The expression of VrDhn1 mRNA and accumulation of VrDhn1 protein were at high levels in mature seeds, but neither mRNA nor protein was detected in mungbean vegetative tissues under normal growth conditions. The VrDhn1 mRNA level was extremely high in mature seeds and decreased to ∼30% at 1 DAI, and was not detectable at ~7 DAI. Tissue dehydration, salinity and exogenous ABA markedly induced VrDhn1 transcripts in plants as measured by quantitative real-time reverse transcription-PCR (qRT-PCR). VrDhn1 protein was not detected using immunoblots in seedlings under stress treatments. In mature seeds or 1 DAI seedlings, VrDhn1 proteins were immunolocalized in the nucleus and cytoplasm. VrDhn1 exhibited low affinity for non-specific interaction with DNA using electrophoretic mobility shift assays (EMSAs), and the exogenous addition of Zn(2+) or Ni(2+) stimulated interaction. The His-tagged VrDhn1 (30.17 kDa) protein showed a molecular mass of 63.1 kDa on gel filtration, suggesting a dimer form. This is the first report showing that a Y(2)K-type VrDhn1 enters the nucleus and interacts with DNA during seed maturation.
authors.affiliation: Institute of Bioinformatics and Structural Biology & Department of Life Science, National Tsing Hua University, 101 Sec 2, Kuang-Fu Road, Hsinchu 30013, Taiwan.
authors.forename: Chia-Hui
authors.initials: CH
authors.lastname: Lin
authors.forename: Po-Hsin
authors.initials: PH
authors.lastname: Peng
authors.forename: Chia-Yun
authors.initials: CY
authors.lastname: Ko
authors.forename: Albert H
authors.initials: AH
authors.lastname: Markhart
authors.forename: Tsai-Yun
authors.initials: TY
authors.lastname: Lin
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D000595:Amino Acid Sequence; D001483:Base Sequence; D002850:Chromatography, Gel; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000378:metabolism; D003890:Desiccation; D024202:Electrophoretic Mobility Shift Assay; D007887:Fabaceae / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D018525:Germination / Q000187:drug effects / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009928:Organ Specificity / Q000187:drug effects / Q000235:genetics; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant; D010940:Plant Proteins; D012333:RNA, Messenger; C070556:dehydrin proteins, plant; D012965:Sodium Chloride; D000040:Abscisic Acid