title: Xenobiotic- and jasmonic acid-inducible signal transduction pathways have become interdependent at the Arabidopsis CYP81D11 promoter.
abstract: Plants modify harmful substances through an inducible detoxification system. In Arabidopsis (Arabidopsis thaliana), chemical induction of the cytochrome P450 gene CYP81D11 and other genes linked to the detoxification program depends on class II TGA transcription factors. CYP81D11 expression is also induced by the phytohormone jasmonic acid (JA) through the established pathway requiring the JA receptor CORONATINE INSENSITIVE1 (COI1) and the JA-regulated transcription factor MYC2. Here, we report that the xenobiotic- and the JA-dependent signal cascades have become interdependent at the CYP81D11 promoter. On the one hand, MYC2 can only activate the expression of CYP81D11 when both the MYC2- and the TGA-binding sites are present in the promoter. On the other hand, the xenobiotic-regulated class II TGA transcription factors can only mediate maximal promoter activity if TGA and MYC2 binding motifs, MYC2, and the JA-isoleucine biosynthesis enzymes DDE2/AOS and JAR1 are functional. Since JA levels and degradation of JAZ1, a repressor of the JA response, are not affected by reactive chemicals, we hypothesize that basal JA signaling amplifies the response to chemical stress. Remarkably, stress-induced expression levels were 3-fold lower in coi1 than in the JA biosynthesis mutant dde2-2, [corrected] revealing that COI1 can contribute to the activation of the promoter in the absence of JA. Moreover, we show that deletion of the MYC2 binding motifs abolishes the JA responsiveness of the promoter but not the responsiveness to COI1. These findings suggest that yet unknown cis-element(s) can mediate COI1-dependent transcriptional activation in the absence of JA.
authors.affiliation: Albrecht-von-Haller-Institut für Pflanzenwissenschaften, Georg-August-Universität Göttingen, D-37077 Gottingen, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Köster
authors.forename: Corinna
authors.initials: C
authors.lastname: Thurow
authors.forename: Kerstin
authors.initials: K
authors.lastname: Kruse
authors.forename: Alexander
authors.initials: A
authors.lastname: Meier
authors.forename: Tim
authors.initials: T
authors.lastname: Iven
authors.forename: Ivo
authors.initials: I
authors.lastname: Feussner
authors.forename: Christiane
authors.initials: C
authors.lastname: Gatz
mesh_terms: D020816:Amino Acid Motifs; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / Q000235:genetics / Q000378:metabolism; D001583:Benzoxazoles / Q000494:pharmacology; D003517:Cyclopentanes / Q000378:metabolism* / Q000494:pharmacology; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D009713:Nucleotidyltransferases / Q000235:genetics / Q000378:metabolism; D054883:Oxylipins / Q000378:metabolism* / Q000494:pharmacology; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D015398:Signal Transduction / Q000187:drug effects*; D015533:Transcriptional Activation; D014170:Transformation, Genetic; D014283:Triiodobenzoic Acids / Q000494:pharmacology; D015262:Xenobiotics / Q000494:pharmacology*; D010453:Peptide Synthases
chemical_list: D029681:Arabidopsis Proteins; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; D001583:Benzoxazoles; D003517:Cyclopentanes; D003577:Cytochrome P-450 Enzyme System; D009713:Nucleotidyltransferases; D054883:Oxylipins; D014283:Triiodobenzoic Acids; D015262:Xenobiotics; C112279:COI1 protein, Arabidopsis; C473611:MYC2 protein, Arabidopsis; C017057:benzoxazolone; C011006:jasmonic acid; C052968:2,3,5-triiodobenzoic acid; C412831:AT2G46370 protein, Arabidopsis; D010453:Peptide Synthases