title: Intravital models of infection lay the foundation for tissue microbiology.
abstract: In complex environments, such as those found in the human host, pathogenic bacteria constantly battle the unfavorable conditions imposed by the host response to their presence. During Escherichia coli-induced pyelonephritis, a cascade of events are shown in an intravital animal model to occur in a timely and sequential manner, representing the dynamic interplay between host and pathogen. Today, intravital techniques allow for observing infection in the living host. At resolutions almost on the single-cell level, improved detection methods offer a movie-like description of infection dynamics. Tissue microbiology involves monitoring host-pathogen interaction within the dynamic microecology of infectious sites in the live host. This new field holds great promise for insightful research into microbial disease intervention strategies.
authors.affiliation: Swedish Medical Nanoscience Center, Department of Neuroscience, Karolinska Institutet, S-17177 Stockholm, Sweden.
authors.forename: Ferdinand X
authors.initials: FX
authors.lastname: Choong
authors.forename: Jakob
authors.initials: J
authors.lastname: Regberg
authors.forename: Klas I
authors.initials: KI
authors.lastname: Udekwu
authors.forename: Agneta
authors.initials: A
authors.lastname: Richter-Dahlfors
mesh_terms: D000818:Animals; D054884:Host-Pathogen Interactions; D006801:Humans; D008954:Models, Biological; D011704:Pyelonephritis / Q000276:immunology / Q000382:microbiology*; D014551:Urinary Tract / Q000276:immunology / Q000382:microbiology*; D056689:Uropathogenic Escherichia coli / Q000276:immunology / Q000502:physiology*