title: [To emphasize the importance of specimen fixation and improve quality of diagnosis of gastrointestinal stromal tumor].
abstract: The most common problem in the diagnosis of gastrointestinal stromal tumor (GIST) is inadequate specimen fixation. The paper focused on specimen fixation and standardized protocol in immunohistochemistry staining and gene mutation detection. We have adjusted some procedure used in immunohistochemistry staining and c-kit gene detection to improve the quality of inadequately fixed specimen. It maybe useful for clinicians, pathologists and technicians working in immunohistochemistry labs and gene detection labs.
authors.affiliation: Department of Patholgoy, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.
authors.forename: Yong-dong
authors.initials: YD
authors.lastname: Liu
authors.forename: Wei-wei
authors.initials: WW
authors.lastname: Zhao
authors.forename: Hui-juan
authors.initials: HJ
authors.lastname: Chen
authors.forename: Han-liang
authors.initials: HL
authors.lastname: Lin
mesh_terms: D046152:Gastrointestinal Stromal Tumors / Q000175:diagnosis* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D009154:Mutation; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics*; D013048:Specimen Handling; D013194:Staining and Labeling
chemical_list: D019009:Proto-Oncogene Proteins c-kit