title: [Study of the state of parietal microflora and wall of the large intestine of mice under the influence of anomalous magnetic field].
abstract: OBJECTIVE
  Study the possible qualitative and quantitative changes of microbial community of the parietal mucin of the large intestine and the state of the wall of the large intestine in experimental animals underbackground and anomalous influence of geomagnetic field.
  
  
  METHODS
  CBA mice were put under the influence of anomalous magnetic field comparable to its intensity in Zheleznogorsk (3 Oe) for 1 and 2 weeks. Quantitative and qualitative study of mucous microflora of the large intestine of the mice was performed by bacteriological method. Identification of the microorganisms was performed by microbiological analyzer "Multiskan-Ascent" and commercial test-systems "Lachema-Czech Republic": ENTHEROtest-16, STAPHYtest-16, Streptotest-16, En-COCCUStest-16; for lactobacilli and bifidobacteria identification - API 50 CHL (bioMerieux). Bacteria content in 1 g of material was calculated by the number of microorganism colonies grown.
  
  
  RESULTS
  A pattern of changes of mucous microflora of the intestine and the state of the wall of the large intestine of the experimental animals that had been put under the influence of anomalous magnetic field is shown. During evaluation of qualitative and quantitative diversity of microbial community of parietal mucin of the large intestine of the mice under the influence of magnetic field on the background and anomalous levels changes not only in quantity and frequency of detection of obligate, transitory flora but also cell elements of mucous membrane of the wall of the large intestine were established.
  
  
  CONCLUSIONS
  The results of the study allow to make a conclusion about the presence of reactivity of the parietal microflora of the intestine of the mice to the influence of the anomalous magnetic field. This leads to changes in cell elements in the mucous membrane of the wall that manifest by infiltration of the connective tissue stroma by leucocytes and reconstruction of epithelium, that are features of dysbiosis.
authors.forename: O A
authors.initials: OA
authors.lastname: Medvedeva
authors.forename: P V
authors.initials: PV
authors.lastname: Kalutskiĭ
authors.forename: A V
authors.initials: AV
authors.lastname: Besedin
authors.forename: L V
authors.initials: LV
authors.lastname: Zhiliaeva
authors.forename: E V
authors.initials: EV
authors.lastname: Ostap
authors.forename: A V
authors.initials: AV
authors.lastname: Ivanov
authors.forename: S K
authors.initials: SK
authors.lastname: Medvedeva
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000528:radiation effects; D015373:Bacterial Typing Techniques; D015169:Colony Count, Microbial; D060733:Electromagnetic Radiation; D006090:Gram-Negative Bacteria / Q000254:growth & development / Q000528:radiation effects*; D006094:Gram-Positive Bacteria / Q000254:growth & development / Q000528:radiation effects*; D006098:Granulocytes / Q000528:radiation effects; D006651:Histocytochemistry; D007413:Intestinal Mucosa / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology / Q000528:radiation effects; D007420:Intestine, Large / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology / Q000528:radiation effects; D060526:Magnetic Fields / Q000009:adverse effects*; D054892:Metagenome / Q000502:physiology*; D051379:Mice; D008808:Mice, Inbred CBA; D009077:Mucins / Q000737:chemistry / Q000378:metabolism; D009093:Mucus / Q000737:chemistry / Q000378:metabolism; D020556:Neutrophil Infiltration / Q000528:radiation effects
chemical_list: D009077:Mucins