title: Molecular epidemiology of an outbreak of imipenem-resistant Acinetobacter baumannii carrying the ISAba1-bla(OXA-51-like) genes in a Korean hospital.
abstract: Between January 2004 and December 2004, an outbreak of imipenem-resistant  Acinetobacter baumannii (IRAB) in 2 intensive care units (ICU) of Chosun University Hospital,  Korea affected 77 patients. A case-control study revealed that the time spent in the hospital and  mechanical ventilation practices were risk factors. IRAB was isolated from the hands of 4% (5/124)  of healthcare workers; 27.3% (21/77) of the samples obtained from the ICU environment. A pulsed-field gel electrophoresis analysis showed that 82.1% (23/28) of clinical  IRAB isolates and 85.7% (6/7) of environmental IRAB isolates were type A. The ISAba1F/OXA-51-likeR  PCR showed that 93.7% (30/32) of IRAB strains had the ISAba1 gene upstream of the bla(OXA-51-like)  gene. Two ISAba1F/OXA-51-likeR PCR-negative IRAB strains were bla(IMP-1) positive. All of the  IRAB strains tested by PCR were negative for bla(VIM), bla(SIM), bla(GIM-1), bla(SPM-1), bla(GES),  bla(OXA-23-like), bla(OXA-24-like), and bla(OXA-58-like) carbapenemase genes. After implementing an  infection control strategy, a steady reduction in the attack rate of IRAB infection was observed.
authors.affiliation: Research Center for Resistant Cells, Chosun University Medical School, Gwang-Ju, Korea.
authors.forename: Bidur Prasad
authors.initials: BP
authors.lastname: Chaulagain
authors.forename: Sook Jin
authors.initials: SJ
authors.lastname: Jang
authors.forename: Gyuu Yeol
authors.initials: GY
authors.lastname: Ahn
authors.forename: So Yeon
authors.initials: SY
authors.lastname: Ryu
authors.forename: Dong Min
authors.initials: DM
authors.lastname: Kim
authors.forename: Geon
authors.initials: G
authors.lastname: Park
authors.forename: Won Yong
authors.initials: WY
authors.lastname: Kim
authors.forename: Jong Hee
authors.initials: JH
authors.lastname: Shin
authors.forename: Joong Ki
authors.initials: JK
authors.lastname: Kook
authors.forename: Seong-Ho
authors.initials: SH
authors.lastname: Kang
authors.forename: Dae Soo
authors.initials: DS
authors.lastname: Moon
authors.forename: Young Jin
authors.initials: YJ
authors.lastname: Park
mesh_terms: D000151:Acinetobacter Infections / Q000453:epidemiology* / Q000382:microbiology; D040981:Acinetobacter baumannii / Q000145:classification* / Q000187:drug effects* / Q000201:enzymology / Q000302:isolation & purification; D016022:Case-Control Studies; D016000:Cluster Analysis; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D005838:Genotype; D006225:Hand / Q000382:microbiology; D006282:Health Personnel; D006761:Hospitals; D006801:Humans; D015378:Imipenem / Q000494:pharmacology*; D007362:Intensive Care Units; D007723:Korea / Q000453:epidemiology; D008297:Male; D017720:Molecular Epidemiology; D058889:Molecular Typing; D012307:Risk Factors; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D015378:Imipenem; D001618:beta-Lactamases