title: Enhanced expression of recombinant human cyclooxygenase 1 from stably-transfected Drosophila melanogaster S2 cells by dimethyl sulfoxide is mediated by up-regulation of nitric oxide synthase and transcription factor Kr-h1.
abstract: Recombinant human cyclooxygenase 1 (COX-1) was expressed from stably-transfected Drosophila melanogaster S2 (S2) cells. DMSO improved the expression of recombinant COX-1 by 180 %. DMSO increased the expression of nitric oxide synthase (NOS) at both the RNA and protein levels; NOS expression was closely correlated with the synthesis of recombinant COX-1 mRNA in stably-transfected S2 cells. DMSO also induced the gene encoding Kr-h1 which binds to the CACCC element of the metallothionein promoter to enhance the expression of recombinant COX-1. Therefore, DMSO improves the expression of recombinant COX-1 via NOS and/or the transcription factor Kr-h1.
authors.affiliation: Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Korea.
authors.forename: Kyung Hwa
authors.initials: KH
authors.lastname: Chang
authors.forename: Jong-Hwa
authors.initials: JH
authors.lastname: Park
authors.forename: Ha Young
authors.initials: HY
authors.lastname: Chung
authors.forename: Jeon
authors.initials: J
authors.lastname: Hwang-Bo
authors.forename: Hyun Ho
authors.initials: HH
authors.lastname: Lee
authors.forename: Do Hyung
authors.initials: DH
authors.lastname: Kim
authors.forename: Yunjo
authors.initials: Y
authors.lastname: Soh
authors.forename: In Sik
authors.initials: IS
authors.lastname: Chung
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D051545:Cyclooxygenase 1 / Q000096:biosynthesis* / Q000235:genetics; D004121:Dimethyl Sulfoxide / Q000378:metabolism*; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster; D015870:Gene Expression; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D051741:Kruppel-Like Transcription Factors / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
chemical_list: D029721:Drosophila Proteins; C497242:Kr-h1 protein, Drosophila; D051741:Kruppel-Like Transcription Factors; D011994:Recombinant Proteins; D019001:Nitric Oxide Synthase; D051545:Cyclooxygenase 1; D004121:Dimethyl Sulfoxide