title: The first International Standard anti-Brucella melitensis Serum.
abstract: The World Organisation for Animal Health (OIE) requested an International Standard anti-Brucella melitensis Serum (ISaBmS) to standardise diagnostic tests and reagents for sheep and goats. The agreed criteria were the highest dilution (in negative serum) of the standard which must give a positive result and the lowest dilution (in negative serum) which must simultaneously give a negative result. The two dilutions for each assay were, respectively: indirect enzyme-linked immunosorbent assay (iELISA) 1/64 and 1/750, competitive ELISA (cELISA) 1/8 and 1/300, fluorescent polarisation assay (FPA) 1/16 and 1/200, Rose Bengal test (RBT) 1/16 and 1/200. The OIE International Standard Serum (OIEISS) will remain the primary standard for the RBT; the ISaBmS is an additional standard. It was impossible to set criteria for the complement fixation test, therefore the OIEISS will remain the primary standard. The ISaBmS can be used to standardise iELISA, cELISA and FPA to diagnose sheep and goat brucellosis. This standard should facilitate harmonisation of tests used for brucellosis surveillance and international trade in these species.
authors.affiliation: Animal Health Veterinary Laboratories Agency (OIE/FAO Reference Laboratory), Woodham Lane, Addlestone, Surrey KT15 3NB, United Kingdom.
authors.forename: J
authors.initials: J
authors.lastname: McGiven
authors.forename: A
authors.initials: A
authors.lastname: Taylor
authors.forename: L
authors.initials: L
authors.lastname: Duncombe
authors.forename: R
authors.initials: R
authors.lastname: Sayers
authors.forename: D
authors.initials: D
authors.lastname: Albert
authors.forename: M
authors.initials: M
authors.lastname: Banai
authors.forename: J M
authors.initials: JM
authors.lastname: Blasco
authors.forename: S
authors.initials: S
authors.lastname: Elena
authors.forename: D
authors.initials: D
authors.lastname: Fretin
authors.forename: B
authors.initials: B
authors.lastname: Garin-Bastuji
authors.forename: F
authors.initials: F
authors.lastname: Melzer
authors.forename: P M
authors.initials: PM
authors.lastname: Muñoz
authors.forename: K
authors.initials: K
authors.lastname: Nielsen
authors.forename: A
authors.initials: A
authors.lastname: Nicola
authors.forename: M
authors.initials: M
authors.lastname: Scacchia
authors.forename: M
authors.initials: M
authors.lastname: Tittarelli
authors.forename: I Travassos
authors.initials: IT
authors.lastname: Dias
authors.forename: K
authors.initials: K
authors.lastname: Walravens
authors.forename: J
authors.initials: J
authors.lastname: Stack
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D017347:Brucella melitensis / Q000276:immunology*; D002006:Brucellosis / Q000175:diagnosis / Q000662:veterinary*; D003168:Complement Fixation Tests / Q000662:veterinary; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D016231:Fluorescence Polarization Immunoassay / Q000662:veterinary; D015511:Goat Diseases / Q000175:diagnosis*; D006041:Goats; D007106:Immune Sera / Q000097:blood*; D011247:Pregnancy; D012015:Reference Standards; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis
chemical_list: D000907:Antibodies, Bacterial; D007106:Immune Sera