title: Membrane topology and functional analysis of Methylobacillus sp. 12S genes epsF and epsG, encoding polysaccharide chain-length determining proteins.
abstract: The EpsF and EpsG of the methanol-assimilating bacterium Methylobacillus sp. 12S are involved in the synthesis of a high molecular weight exopolysaccharide, methanolan. These proteins share homology with chain-length determiners in other polysaccharide-producing bacteria. The N- and C-termini of EpsF were found to locate to the cytoplasm, and EpsF was predicted to have two transmembrane regions. EpsG showed both ATPase and autophosphorylation activities.
authors.affiliation: Biotechnology Research Center, The University of Tokyo, Tokyo, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Ayabe-Chujo
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Usami
authors.forename: Takako
authors.initials: T
authors.lastname: Yoshida
authors.forename: Toshio
authors.initials: T
authors.lastname: Omori
authors.forename: Hideaki
authors.initials: H
authors.lastname: Nojiri
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D020631:Methylobacillus / Q000166:cytology* / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000096:biosynthesis* / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D011134:Polysaccharides