title: Direct drug metabolism monitoring in a live single hepatic cell by video mass spectrometry.
abstract: The metabolism of anti-breast cancer drug, tamoxifen, in a single human hepatocellular carcinoma cell, HepG2, was directly monitored by a video-mass spectroscope.  The cytoplasm, a vacuole or nucleus of the cell was directly sucked by a nano-spray tip under a video-microscope, and then was introduced into a mass spectrometer.  Unchanged drug molecules were found in cytoplasm and a vacuole, but the metabolites were only found in the cytoplasm.  This direct detection of drug metabolites in a live single cell is useful for speedy drug metabolism monitoring.
authors.affiliation: Quantitative Biology Center, RIKEN, Suita, Osaka, Japan.
authors.forename: Sachiko
authors.initials: S
authors.lastname: Date
authors.forename: Hajime
authors.initials: H
authors.lastname: Mizuno
authors.forename: Naohiro
authors.initials: N
authors.lastname: Tsuyama
authors.forename: Takanori
authors.initials: T
authors.lastname: Harada
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Masujima
mesh_terms: D000970:Antineoplastic Agents / Q000378:metabolism*; D002470:Cell Survival; D056945:Hep G2 Cells; D006801:Humans; D008099:Liver / Q000166:cytology*; D013058:Mass Spectrometry / Q000191:economics / Q000379:methods*; D059010:Single-Cell Analysis / Q000191:economics / Q000379:methods*; D013629:Tamoxifen / Q000378:metabolism*; D013997:Time Factors
chemical_list: D000970:Antineoplastic Agents; D013629:Tamoxifen