title: Yeast transformation to generate high-yielding clones.
abstract: There are several ways to introduce non-native DNA into yeast cells, including chemical transformation and electroporation. Methods for both of these procedures are outlined in this chapter. Both methods permit the uptake of DNA from the environment through yeast cell membranes and this DNA can be episomally maintained or integrated into the host genome. However, yeast cells must first be made competent to permit passive entry of the DNA and various methods are outlined in this chapter to facilitate this. All of the described methods can be applied in combination with antibiotic or auxotrophic selection pressure.
authors.affiliation: School of Biosciences, University of Birmingham, Birmingham, West Midlands, UK.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Jamshad
authors.forename: Richard A J
authors.initials: RA
authors.lastname: Darby
mesh_terms: D018274:Electroporation; D010843:Pichia / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D014170:Transformation, Genetic / Q000235:genetics*