title: Walking nanothermometers: spatiotemporal temperature measurement of transported acidic organelles in single living cells.
abstract: We fabricated fluorescent nanoparticles which monitor temperature changes without sensitivity to pH (4-10) and ionic strength (0-500 mM). The nanothermometers spontaneously enter living HeLa cells via endocytosis, enclosed in acidic organelles, i.e., endosome/lysosome, and then transported along microtubules in a temperature-dependent manner, working as "walking nanothermometers".
authors.affiliation: Department of Physics, School of Advanced Science and Engineering, Waseda University, Tokyo, Japan.
authors.forename: Kotaro
authors.initials: K
authors.lastname: Oyama
authors.forename: Masao
authors.initials: M
authors.lastname: Takabayashi
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Takei
authors.forename: Satoshi
authors.initials: S
authors.lastname: Arai
authors.forename: Shinji
authors.initials: S
authors.lastname: Takeoka
authors.forename: Shin'ichi
authors.initials: S
authors.lastname: Ishiwata
authors.forename: Madoka
authors.initials: M
authors.lastname: Suzuki
mesh_terms: D011992:Endosomes / Q000737:chemistry; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008247:Lysosomes / Q000737:chemistry*; D018613:Microscopy, Confocal; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000295:instrumentation*; D009994:Osmolar Concentration; D059010:Single-Cell Analysis / Q000295:instrumentation* / Q000379:methods*; D013821:Thermometers
chemical_list: D005456:Fluorescent Dyes; D016650:Fluorescein-5-isothiocyanate