title: Content of total homocysteine and major aminothiols in rats with experimental renal ischemia.
abstract: The contents of total homocysteine, cysteine, and glutathione in blood plasma and tissue of rats with renal ischemia were measured by HPLC. Our study was performed on the "two-kidney, one-clip (0.13 mm)" model. The concentrations of homocysteine and cysteine in blood plasma from treated rats were higher than in sham-operated animals (control; by 36 and 14%, respectively). Homocysteine level in the intact and clipped kidneys of treated rats was 40% higher than in the control. However, no differences were found in homocysteine level in the ischemic and intact kidneys of treated animals. Cysteine concentration in the clipped kidney was lower than in the kidneys of intact and sham-operated animals (by 1.6 and 1.5 times, respectively). Glutathione concentration in the ischemic kidney did not differ from the control. No differences were revealed in the content of aminothiols in liver samples from rats of the treatment and control groups. Our results suggest that functional inactivation of one kidney is accompanied by impairment of homocysteine catabolism (trans-sulfonation).
authors.affiliation: I. P. Pavlov St. Petersburg Medical University, St. Petersburg, Russia.
authors.forename: N V
authors.initials: NV
authors.lastname: Kuz'menko
authors.forename: L A
authors.initials: LA
authors.lastname: Alexandrova
authors.forename: S G
authors.initials: SG
authors.lastname: Chefu
authors.forename: E L
authors.initials: EL
authors.lastname: Blashko
authors.forename: A A
authors.initials: AA
authors.lastname: Zhloba
authors.forename: V A
authors.initials: VA
authors.lastname: Cirlin
mesh_terms: D000818:Animals; D001794:Blood Pressure; D003545:Cysteine / Q000097:blood; D005978:Glutathione / Q000097:blood; D006710:Homocysteine / Q000097:blood*; D020138:Hyperhomocysteinemia / Q000097:blood* / Q000150:complications / Q000503:physiopathology; D007511:Ischemia / Q000097:blood* / Q000150:complications / Q000503:physiopathology; D007668:Kidney / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012077:Renal Artery / Q000503:physiopathology / Q000601:surgery; D012078:Renal Artery Obstruction / Q000503:physiopathology / Q000601:surgery
chemical_list: D006710:Homocysteine; D005978:Glutathione; D003545:Cysteine