title: IDO-competent-DCs induced by IFN-γ attenuate acute rejection in rat liver transplantation.
abstract: OBJECTIVE
  We established a stable rat model of liver transplantation using Sprague-Dawley rats and Wistar rats in order to investigate the role of the IDO gene in acute rejection after rat liver transplantation.
  
  
  METHODS
  IDO gene expression and IDO enzyme activity were quantified in liver syngeneic grafts and allografts using microdialysis-HPLC. Liver allografts were evaluated for IDO expression by histopathology. We measured liver function-related biomarkers in liver allografts which were re-infused with untreated or IFN-γ-treated dendritic cells (DCs).
  
  
  RESULTS
  We found a significant increase in IDO gene expression and IDO enzyme activity in liver allografts compared the sham and syngeneic graft groups. There was a significant correlation between the number of IDO-positive cells and severity of acute rejection. IDO gene expression and enzyme activity was upregulated in the IFN-γ-treated DC group within 7 days after transplantation compared to the untreated DC group and survival rates were significantly improved.
  
  
  CONCLUSIONS
  Our results suggested that IDO gene expression correlates with the severity of acute rejection and that IFN-γ-induced IDO-positive DCs may attenuate acute rejection and catalyze local tryptophan metabolism via IDO enzyme expression, leading to immune tolerance after liver transplantation.
authors.affiliation: Department of Surgery, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, 100 Haining Road, Shanghai, 200080, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Sun
authors.forename: Zi-jun
authors.initials: ZJ
authors.lastname: Gong
authors.forename: Zhao-wen
authors.initials: ZW
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.forename: Jin-yan
authors.initials: JY
authors.lastname: Zhang
authors.forename: Hong-cheng
authors.initials: HC
authors.lastname: Sun
authors.forename: Shuang
authors.initials: S
authors.lastname: Liu
authors.forename: Li
authors.initials: L
authors.lastname: Huang
authors.forename: Chen
authors.initials: C
authors.lastname: Huang
authors.forename: Zhi-hai
authors.initials: ZH
authors.lastname: Peng
mesh_terms: D000818:Animals; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism*; D006084:Graft Rejection; D007108:Immune Tolerance; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase / Q000096:biosynthesis / Q000235:genetics*; D007371:Interferon-gamma / Q000276:immunology*; D008099:Liver / Q000378:metabolism; D016031:Liver Transplantation / Q000276:immunology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D014184:Transplantation, Homologous; D014364:Tryptophan / Q000378:metabolism
chemical_list: D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D007371:Interferon-gamma; D014364:Tryptophan