title: Soy peptides enhance heterologous membrane protein productivity during the exponential growth phase of Saccharomyces cerevisiae.
abstract: In this study, the production of eight G protein-coupled receptors by Saccharomyces cerevisiae was compared using two types of media, one of which contained soy peptides and the other free amino acids. Yeast cell growth improved in the medium with soy peptides, and the expression levels of six of the receptors increased during the exponential phase by an average of 2.3-fold as against the free amino acid-based medium. The enhancement of protein expression by soy peptides can be explained by alleviation of metabolite stress due to amino acid source depletion caused by heterologous protein expression.
authors.affiliation: Department of Food and Nutritional Sciences, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, Suruga-ku, Shizuoka, Japan. sukeito@u-shizuoka-ken.ac.jp
authors.forename: Keisuke
authors.initials: K
authors.lastname: Ito
authors.forename: Aya
authors.initials: A
authors.lastname: Hikida
authors.forename: Sayuri
authors.initials: S
authors.lastname: Kitagawa
authors.forename: Takumi
authors.initials: T
authors.lastname: Misaka
authors.forename: Keiko
authors.initials: K
authors.lastname: Abe
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Kawarasaki
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D010446:Peptide Fragments / Q000494:pharmacology*; D043562:Receptors, G-Protein-Coupled / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D030262:Soybean Proteins / Q000737:chemistry*
chemical_list: D010446:Peptide Fragments; D043562:Receptors, G-Protein-Coupled; D030262:Soybean Proteins