title: Small non-coding RNA SraG regulates the operon YPK_1206-1205 in Yersinia pseudotuberculosis.
abstract: Small non-coding RNAs (sRNAs) are important post-transcriptional regulators in prokaryotes and have been demonstrated to participate in most of the cellular processes. SraG is an sRNA found in several enterobacterial species, but its targets have not been characterized. Here, we compared the protein expression patterns between the wild-type and an sraG-depleted mutant of Yersinia pseudotuberculosis by proteomic analysis. Sixteen proteins were up- or downregulated, and the negative regulatory role of SraG associated with the YPK_1206-1205 operon was confirmed. A region in the coding sequence of YPK_1206 was further demonstrated to be required for this negative regulation.
authors.affiliation: Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, The Chinese Academy of Sciences, Wuhan, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Lu
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Lamei
authors.initials: L
authors.lastname: Li
authors.forename: Yangbo
authors.initials: Y
authors.lastname: Hu
authors.forename: Li
authors.initials: L
authors.lastname: Huang
authors.forename: Yunlong
authors.initials: Y
authors.lastname: Li
authors.forename: Simon
authors.initials: S
authors.lastname: Rayner
authors.forename: Shiyun
authors.initials: S
authors.lastname: Chen
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis; D017353:Gene Deletion; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D009876:Operon; D020543:Proteome / Q000032:analysis; D058727:RNA, Small Untranslated / Q000235:genetics* / Q000378:metabolism; D015011:Yersinia pseudotuberculosis / Q000235:genetics* / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D020543:Proteome; D058727:RNA, Small Untranslated