title: Massilia namucuonensis sp. nov., isolated from a soil sample.
abstract: A Gram-staining-negative, rod-shaped and non-spore-forming bacterium, designated strain 333-1-0411(T), was isolated from a soil sample collected from Namucuo, Tibet Autonomous Region, China and characterized in a taxonomic study using a polyphasic approach. The major fatty acid components of strain 333-1-0411(T) were summed feature 3 (C(16 : 1)ω7c and/or C(16 : 1)ω6c) and C(16 : 0); its major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. Q-8 was the dominant ubiquinone, and the G+C content of the genomic DNA was 66.7 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain 333-1-0411(T) fell within the evolutionary radiation encompassed by the genus Massilia. The 16S rRNA gene sequence similarities between strain 333-1-0411(T) and recognized species of the genus Massilia ranged from 95.4 % to 97.2 %, and the most closely related strains were Massilia flava Y9(T) (97.2 %) and Massilia albidiflava 45(T) (97.0 %). However, the DNA-DNA relatedness values between strain 333-1-0411(T) and M. flava Y9(T) and M. albidiflava 45(T) were 20.2 % and 27.2 %, respectively. Based on the phenotypic, chemotaxonomic and phylogenetic properties, strain 333-1-0411(T) is considered to represent a novel species of the genus Massilia, for which the name Massilia namucuonensis sp. nov. is proposed. The type strain is 333-1-0411(T) (= CGMCC 1.11014(T) = DSM 25159(T)).
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, PR China.
authors.forename: Bi He
authors.initials: BH
authors.lastname: Kong
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, PR China.
authors.forename: Yan Hong
authors.initials: YH
authors.lastname: Li
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: New Technique Centre, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Chun Li
authors.initials: CL
authors.lastname: Li
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, PR China.
authors.forename: Zhi Wei
authors.initials: ZW
authors.lastname: Yang
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, PR China.
authors.forename: Rong
authors.initials: R
authors.lastname: Yu
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D042802:Oxalobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D018609:Tibet; D014451:Ubiquinone / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C030778:ubiquinone 8