title: Quinolone-induced upregulation of osteopontin gene promoter activity in human lung epithelial cell line A549.
abstract: Quinolones, in addition to their antibacterial activities, act as immunomodulators. Osteopontin (OPN), a member of the extracellular matrix proteins, was found to play a role in the immune and inflammatory response. We found that quinolones significantly enhanced OPN secretion, namely, garenoxacin (220%), moxifloxacin (62%), gatifloxacin (82%), sparfloxacin, (79%), and sitafloxacin (60%). Enhancement of OPN secretion was shown to be due to the effect of quinolones on the OPN gene promoter activity. We also examined the role of quinolones on apoptosis and found that sparfloxacin decreased the late apoptosis of A549 cells, but garenoxacin did not show the antiapoptotic effect. The antiapoptotic effects of quinolones do not appear to be associated with OPN elevation.
authors.affiliation: Division of Emerging and Infectious Diseases, Graduate School of Medicine, Tohoku University, Sendai, Japan.
authors.forename: Beata
authors.initials: B
authors.lastname: Shiratori
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Osamu
authors.initials: O
authors.lastname: Usami
authors.forename: Haorile
authors.initials: H
authors.lastname: Chagan-Yasutan
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Chie
authors.initials: C
authors.lastname: Nakajima
authors.forename: Toshimitsu
authors.initials: T
authors.lastname: Uede
authors.forename: Toshio
authors.initials: T
authors.lastname: Hattori
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D005434:Flow Cytometry; D006801:Humans; D053495:Osteopontin / Q000235:genetics* / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000187:drug effects* / Q000235:genetics*; D015363:Quinolones / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D015363:Quinolones; D053495:Osteopontin