title: Application of amplified ribosomal DNA restriction analysis in identification of Acinetobacter baumannii from a tertiary teaching hospital, Malaysia.
abstract: Acinetobacter baumannii, genomic species 3 and 13TU are being increasingly   reported as the most important Acinetobacter species that cause infections in hospitalized   patients. These Acinetobacter species are grouped in the Acinetobacter calcoaceticus-   Acinetobacter baumannii (Acb) complex. Differentiation of the species in the Acb-complex   is limited by phenotypic methods. Therefore, in this study, amplified ribosomal DNA restriction   analysis (ARDRA) was applied to confirm the identity A. baumannii strains as well as to   differentiate between the subspecies. One hundred and eighty-five strains from Intensive   Care Unit, Universiti Malaya Medical Center (UMMC) were successfully identified as A.   baumannii by ARDRA. Acinetobacter genomic species 13TU and 15TU were identified in 3   and 1 strains, respectively. ARDRA provides an accurate, rapid and definitive approach   towards the identification of the species level in the genus Acinetobacter. This paper reports   the first application ARDRA in genospecies identification of Acinetobacter in Malaysia.
authors.affiliation: Microbiology Division, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: B H
authors.initials: BH
authors.lastname: Kong
authors.forename: Y A
authors.initials: YA
authors.lastname: Hanifah
authors.forename: M Y
authors.initials: MY
authors.lastname: Yusof
authors.forename: K L
authors.initials: KL
authors.lastname: Thong
mesh_terms: D000151:Acinetobacter Infections / Q000175:diagnosis*; D040981:Acinetobacter baumannii / Q000235:genetics / Q000302:isolation & purification*; D001431:Bacteriological Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D006784:Hospitals, Teaching; D006801:Humans; D007362:Intensive Care Units; D008296:Malaysia; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal