title: rs4215 SNP in zinc-α2-glycoprotein gene is associated with obesity in Chinese north Han population.
abstract: OBJECTIVE
  Zinc-α2-glycoprotein (ZAG) has been identified recently as a novel adipokine due to its close link with lipid and glucose metabolism, as well as regulation of body weight. The aim of our present study is to investigate the ZAG genetic polymorphism association with obesity in Chinese north Han population.
  
  
  METHODS
  Five SNPs of ZAG gene including rs2247607 (A>T), rs4727442 (G>T), rs4215 (A>G), rs2527923 (C>T) and rs2527882 (C>T) were genotyped in 648 overweight/obese patients and 313 healthy controls by TaqMan-PCR methods. Crosstabs statistical analysis method with subjects stratifying by age (≦ 30 y, 31-45 y, ≧ 46 y) and gender was used.
  
  
  RESULTS
  The results showed the constitution of three genotype frequencies in rs4215 (A>G) site significantly differs in male subgroup (aged 31-45 y) between overweight/obese and healthy control group (χ(2)=6.401, P=0.041). GG genotype frequency in overweight/obese group is 19.3% which is much higher than 6.1% in healthy control group. Further statistical analysis under a recessive inheritance model demonstrated odd ratio (OR) for GG vs. AA+AG in overweight/obese group was 3.674 (95% CI 1.049-12.866; P=0.035). Among three genotypes of rs4215, the subjects with GG genotype have much more higher body weight, BMI, waist circumference and SBP.
  
  
  CONCLUSIONS
  Our data, for the first time, suggest the genotypes of rs4215 in ZAG gene are significantly associated with obesity in Chinese north Han population. GG genotype subjects in rs4215 site have an increased susceptibility to obesity when compared with the AA+AG genotype subjects.
authors.affiliation: Department of Endocrinology, Key Laboratory of Endocrinology of Ministry of Health, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China.
authors.forename: Hui-Juan
authors.initials: HJ
authors.lastname: Zhu
authors.forename: Cai-Xia
authors.initials: CX
authors.lastname: Dong
authors.forename: Hui
authors.initials: H
authors.lastname: Pan
authors.forename: Xiao-Chuan
authors.initials: XC
authors.lastname: Ping
authors.forename: Nai-Shi
authors.initials: NS
authors.lastname: Li
authors.forename: Yu-Fei
authors.initials: YF
authors.lastname: Dai
authors.forename: Lin-Jie
authors.initials: LJ
authors.lastname: Wang
authors.forename: Hong-Bo
authors.initials: HB
authors.lastname: Yang
authors.forename: Wei-Gang
authors.initials: WG
authors.lastname: Zhao
authors.forename: Feng-Ying
authors.initials: FY
authors.lastname: Gong
mesh_terms: D054392:Adipokines / Q000235:genetics*; D000328:Adult; D044466:Asian People / Q000235:genetics*; D001483:Base Sequence; D001835:Body Weight / Q000235:genetics*; D001837:Body Weights and Measures; D016022:Case-Control Studies; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009765:Obesity / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D029589:Seminal Plasma Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D000090203:Zn-Alpha-2-Glycoprotein
chemical_list: D054392:Adipokines; D029589:Seminal Plasma Proteins; D000090203:Zn-Alpha-2-Glycoprotein