title: Statistical analysis of clone formation in cultures of human stem cells.
abstract: We performed a statistical analysis of clone formation from aneuploid cells (chromosomes 6, 8, 11, X) in cultures of bone marrow-derived human multipotent mesenchymal stromal cells by spontaneous level of aneuploidy at different terms of culturing (from 2 to 19 cell cycles). It was found that the duration of cell cycle increased from 65.6 h at passages 2-3 to 164.5 h at passage 12. The expected ratio of aneuploid cells was calculated using modeled 5, 10, 20 and 30% selective preference in reproduction. The size of samples for detecting 10, 25, and 50% increased level of aneuploidy was calculated. The presented principles for evaluation of aneuploid clone formation may be used to distinguish clones of any abnormal cells.
authors.affiliation: Medical Genetic Research Center, Russian Academy of Medical Sciences, Moscow, Russia.
authors.forename: N P
authors.initials: NP
authors.lastname: Bochkov
authors.forename: M S
authors.initials: MS
authors.lastname: Vinogradova
authors.forename: I K
authors.initials: IK
authors.lastname: Volkov
authors.forename: E S
authors.initials: ES
authors.lastname: Voronina
authors.forename: N P
authors.initials: NP
authors.lastname: Kuleshov
mesh_terms: D000782:Aneuploidy; D002478:Cells, Cultured; D002999:Clone Cells / Q000166:cytology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*