title: Complete monosaccharide analysis by high-performance anion-exchange chromatography with pulsed amperometric detection.
abstract: Monosaccharide analysis is a critical way to profile the composition of complex carbohydrates. Methods to analyze neutral and amino sugars have been established for a long time, but methods for acidic sugars are rare. The acidic sugars, including uronic acids and sialic acids, are also important components in some complex carbohydrates. In this report, a high-performance anion-exchange chromatography method with pulsed amperometric detection was initially developed to analyze acidic sugars including different uronic acids and sialic acids. Subsequently, a method to profile complete monosaccharides, including most neutral, amino, and acidic sugars, was developed. This method has a limit of quantitation of ~12.5 × 10(-3) nmol for each sugar as well as good linearity over a wide range. This is a convenient procedure because it avoids additional derivatization of monosaccharides and has a broad application to a wide range of complex carbohydrates. The monosaccharide compositions of a variety of complex carbohydrates such as different glycosaminoglycans, alginate, fucoidan, and glycans were profiled by this comprehensive method. In addition, the hydrolysis patterns of these complex carbohydrates are discussed.
authors.affiliation: Baxter Healthcare Corporation, Round Lake, Illinois 60073, USA. zhenqing_zhang@baxter.com
authors.forename: Zhenqing
authors.initials: Z
authors.lastname: Zhang
authors.forename: Nazeer M
authors.initials: NM
authors.lastname: Khan
authors.forename: Karen M
authors.initials: KM
authors.lastname: Nunez
authors.forename: Edward K
authors.initials: EK
authors.lastname: Chess
authors.forename: Christina M
authors.initials: CM
authors.lastname: Szabo
mesh_terms: D000464:Alginates / Q000737:chemistry; D000838:Anions / Q000737:chemistry; D002240:Carbohydrate Sequence; D002852:Chromatography, Ion Exchange / Q000379:methods*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D057230:Limit of Detection; D016014:Linear Models; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry
chemical_list: D000464:Alginates; D000838:Anions; D006603:Hexuronic Acids; D009005:Monosaccharides; D011134:Polysaccharides; D020723:Glucuronic Acid; C007789:fucoidan