title: Evaluation and comparison of native and recombinant LipL21 protein-based ELISAs for diagnosis of bovine leptospirosis.
abstract: A 21-kDa leptospiral lipoprotein (LipL21) was evaluated for its diagnostic potential to detect bovine leptospirosis by ELISA. Both native LipL21 (nLipL21) and recombinant LipL21 (rLipL21) proteins were tested and compared regarding diagnostic efficiency, and no statistically significant difference was observed. The sensitivity of rLipL21 ELISA for 62 microscopic agglutination test (MAT) positive sera was 100% and the specificity with 378 MAT negative sera was 97.09%. Thus, rLipL21 protein-based ELISA could be used as an alternative to MAT for the diagnosis of bovine leptospirosis.
authors.affiliation: Indian Veterinary Research Institute, Bareilly 243122, U.P., India. siju96@gmail.com
authors.forename: Siju
authors.initials: S
authors.lastname: Joseph
authors.forename: Naicy
authors.initials: N
authors.lastname: Thomas
authors.forename: E
authors.initials: E
authors.lastname: Thangapandian
authors.forename: Vijendra P
authors.initials: VP
authors.lastname: Singh
authors.forename: Rishendra
authors.initials: R
authors.lastname: Verma
authors.forename: S K
authors.initials: SK
authors.lastname: Srivastava
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000382:microbiology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D007921:Leptospira interrogans / Q000302:isolation & purification*; D007922:Leptospirosis / Q000097:blood / Q000382:microbiology / Q000662:veterinary*; D008074:Lipoproteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D012680:Sensitivity and Specificity
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; C545585:LipL21 protein, Leptospira interrogans; D008074:Lipoproteins; D011994:Recombinant Proteins