title: ARTD1 deletion causes increased hepatic lipid accumulation in mice fed a high-fat diet and impairs adipocyte function and differentiation.
abstract: ADP-ribosyltransferase Diphtheria toxin-like 1 [ARTD1; formerly called poly-ADP-ribose polymerase 1 (PARP1)] is a chromatin-associated enzyme involved in regulating metabolic homeostasis. The liver is at the core of glucose and lipid metabolism and is significantly affected by obesity and the metabolic syndrome. Here, we show that when fed a high-fat diet (HFD), mice lacking ARTD1 developed exacerbated hepatic steatosis. ARTD1(-/-) mice had a 19% higher liver weight than wild-type (WT) animals and exhibited a significantly increased serum concentration of cholesterol (38%) and impaired glucose tolerance. In addition, adipocyte function and size were significantly reduced in ARTD1(-/-) mice fed an HFD (7794 μm(2) for WT and 5579 μm(2) for ARTD1(-/-) mice). The significantly reduced adipogenic differentiation of adipose-derived stromal cells (ASCs) isolated from ARTD1(-/-) mice (28 vs. 11% Oil red O-positive cells in WT and ARTD1(-/-) ASCs, respectively) suggested that impaired adipogenesis as the underlying cause for this adipose tissue malfunction. This function of ARTD1 was specific for adipogenesis, since osteogenic differentiation was not affected by the ARTD1 deletion. In summary, we show that ARTD1(-/-) mice fed an HFD display impaired adipogenesis and show exacerbated hepatic steatosis, which can have important implications for nonalcoholic fatty liver disease.
authors.affiliation: Institute of Veterinary Biochemistry and Molecular Biology, University of Zürich, Winterthurerstrasse 190, Zürich 8057, Switzerland.
authors.forename: Süheda
authors.initials: S
authors.lastname: Erener
authors.forename: Ali
authors.initials: A
authors.lastname: Mirsaidi
authors.forename: Mareike
authors.initials: M
authors.lastname: Hesse
authors.forename: André N
authors.initials: AN
authors.lastname: Tiaden
authors.forename: Helga
authors.initials: H
authors.lastname: Ellingsgaard
authors.forename: Radina
authors.initials: R
authors.lastname: Kostadinova
authors.forename: Marc Y
authors.initials: MY
authors.lastname: Donath
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Richards
authors.forename: Michael O
authors.initials: MO
authors.lastname: Hottiger
mesh_terms: D017667:Adipocytes / Q000378:metabolism; D050156:Adipogenesis; D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation; D002784:Cholesterol / Q000097:blood; D059305:Diet, High-Fat; D005234:Fatty Liver / Q000209:etiology*; D018149:Glucose Intolerance / Q000209:etiology; D008099:Liver / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D065626:Non-alcoholic Fatty Liver Disease; D010012:Osteogenesis; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases / Q000172:deficiency / Q000235:genetics*
chemical_list: D002784:Cholesterol; C499321:Parp1 protein, mouse; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases