title: In vitro effects of anandamide and prostamide e2 on normal and transformed nerve cells.
abstract: We studied the effects of endocannabinoid anandamide and its cyclooxygenase derivative prostamide E2 on cultured cerebellar granular cells and C6 glioma cells from rats. Prostamide E2 prevented apoptosis in cerebellar neurons induced by potassium deprivation of cultures, while anandamide had no neuroprotective properties. Prostamide E2 did not modulate the survival rate of glioma cells, while anandamide produced a cytotoxic effect. Our results indicate that cyclooxygenase transformation of anandamide is followed by the loss of antitumor activity of this agent. By contrast, prostamide E2 exhibited strong neuroprotective properties.
authors.affiliation: Laboratory of Experimental Neurocytology, Neurology Research Center, Russian Academy of Medical Sciences, Moscow, Russia.
authors.forename: E L
authors.initials: EL
authors.lastname: Andrianova
authors.forename: E E
authors.initials: EE
authors.lastname: Genrikhs
authors.forename: M Yu
authors.initials: MY
authors.lastname: Bobrov
authors.forename: A A
authors.initials: AA
authors.lastname: Lizhin
authors.forename: N M
authors.initials: NM
authors.lastname: Gretskaya
authors.forename: L E
authors.initials: LE
authors.lastname: Frumkina
authors.forename: L G
authors.initials: LG
authors.lastname: Khaspekov
authors.forename: V V
authors.initials: VV
authors.lastname: Bezuglov
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001095:Arachidonic Acids / Q000378:metabolism* / Q000494:pharmacology; D001711:Biotransformation; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D015232:Dinoprostone / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D063388:Endocannabinoids; D005910:Glioma / Q000378:metabolism / Q000473:pathology; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D018696:Neuroprotective Agents / Q000378:metabolism / Q000494:pharmacology*; D053284:Polyunsaturated Alkamides / Q000378:metabolism* / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism / Q000494:pharmacology*; D011191:Potassium Deficiency / Q000378:metabolism; D061251:Primary Cell Culture; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D051381:Rats
chemical_list: D001095:Arachidonic Acids; D063388:Endocannabinoids; D018696:Neuroprotective Agents; D053284:Polyunsaturated Alkamides; C107684:prostaglandin E2 ethanolamide; D011451:Prostaglandin-Endoperoxide Synthases; D015232:Dinoprostone; D011188:Potassium; C078814:anandamide