title: The collapsin response mediator protein 5 onconeural protein is expressed in Schwann cells under axonal signals and regulates axon-Schwann cell interactions.
abstract: Collapsin response mediator protein 5 (CRMP5) is one of the rare peripheral nerve antigens that is a target of autoantibodies in a paraneoplastic peripheral neuropathy. The pattern of axonal and myelin alterations suggests that CRMP5 is involved in axon-Schwann cell interaction. We examined CRMP5 expression and function in primary cultures of Schwann cells and neurons and at various developmental and regenerating stages of rat sciatic nerve and in CRMP5-deficient mice in vivo. Collapsin response mediator protein 5 was strongly expressed during postnatal development and regeneration and decreased with myelination. It was mainly expressed by immature Schwann cells and persisted in Remak cells in the adult; however, a subpopulation of Schwann cells that were induced to myelinate also expressed CRMP5. We identified 2 axonal molecular cues regulating CRMP5 expression: human neuregulin type 1, which induces CRMP5 expression in immature and premyelinating Schwann cells, and cyclic adenosine monophosphate, which inhibits CRMP5 expression when Schwann cells begin myelination. Collapsin response mediator protein 5-deficient mice showed abnormal Schwann process extension resulting in abnormal cell-axon segregation, indicating that CRMP5 is involved in the morphologic adaptation of Schwann cells to surround axons. These results demonstrate the importance of CRMP5 in axon-Schwann cell cooperation during development and regeneration.
authors.affiliation: Université de Lyon, Saint-Etienne, France. j.philippe.camdessanche@chu-st-etienne.fr
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Camdessanché
authors.forename: Karine
authors.initials: K
authors.lastname: Ferraud
authors.forename: Nadia
authors.initials: N
authors.lastname: Boutahar
authors.forename: François
authors.initials: F
authors.lastname: Lassablière
authors.forename: Marie
authors.initials: M
authors.lastname: Mutter
authors.forename: Monique
authors.initials: M
authors.lastname: Touret
authors.forename: Pappachan
authors.initials: P
authors.lastname: Kolattukudy
authors.forename: Jérôme
authors.initials: J
authors.lastname: Honnorat
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Antoine
mesh_terms: D000581:Amidohydrolases / Q000096:biosynthesis* / Q000502:physiology*; D000818:Animals; D000831:Animals, Newborn; D001369:Axons / Q000502:physiology*; D002450:Cell Communication / Q000502:physiology*; D002478:Cells, Cultured; D018920:Coculture Techniques; D005786:Gene Expression Regulation; D006801:Humans; D006867:Hydrolases; D051379:Mice; D018345:Mice, Knockout; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000502:physiology; D051381:Rats; D012583:Schwann Cells / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology
chemical_list: D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; C501733:Dpysl5 protein, mouse; D006867:Hydrolases; D000581:Amidohydrolases