title: Lipase and phospholipase inhibitors: design and applications.
abstract: Lipases are important drug discovery targets since they play central roles in signal transduction and metabolism. Many inhibitors have been isolated from natural sources and others derived through synthesis of substrate analogs or biomimetics. Because lipids are hydrophobic, there are special procedures needed for their study. This chapter covers two commonly used procedures for assay of lipases and phospholipases and how they can be adapted for the discovery and analysis of inhibitors.
authors.affiliation: Department of Biology, Appalachian State University, Boone, NC, USA. venableme@appstate.edu
authors.forename: Mark E
authors.initials: ME
authors.lastname: Venable
mesh_terms: D000818:Animals; D001681:Biological Assay; D040761:Biomimetic Materials / Q000138:chemical synthesis / Q000494:pharmacology; D015195:Drug Design; D055808:Drug Discovery; D004791:Enzyme Inhibitors / Q000138:chemical synthesis / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007700:Kinetics; D008049:Lipase / Q000037:antagonists & inhibitors* / Q000737:chemistry; D008055:Lipids / Q000737:chemistry; D010740:Phospholipases / Q000037:antagonists & inhibitors* / Q000737:chemistry; D013379:Substrate Specificity
chemical_list: D004791:Enzyme Inhibitors; D008055:Lipids; D010740:Phospholipases; D008049:Lipase