title: Isolation of high quality and yield of RNA from Agaricus bisporus with a simple, inexpensive and reliable method.
abstract: A method for isolating high purity and quantity RNA from Agaricus bisporus which is rich in proteins, carbohydrate, fiber and secondary metabolites, is described. RNA was extracted from mycelium, primordia, sporophores at two development stages and two post-harvest storage stages as well as from pileipellis, inner cap, gill and stipe of the mature sporophore. The A(260)/A(230) and A(260)/A(280) ratios of isolated RNA from fruiting bodies were both ~2 and the yield was about 200 μg/g fresh wt (FW). The yield of RNA from mycelium was approx. 100 μg/g FW. High quality RNA was also extracted from fruiting body tissues of Lentinus edodes, Pleurotus ostreatus, Flammulina velutipes and Pleurotus eryngii with yields from 130 to 225 μg/g FW. RNA extracted from all samples was intact, as demonstrated by gel electrophoresis and was suitable for downstream molecular applications, including RT-PCR and qPCR.
authors.affiliation: College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
authors.forename: De-Mei
authors.initials: DM
authors.lastname: Meng
authors.forename: Lin
authors.initials: L
authors.lastname: Shen
authors.forename: Xin-Hua
authors.initials: XH
authors.lastname: Zhang
authors.forename: Ji-Ping
authors.initials: JP
authors.lastname: Sheng
mesh_terms: D000363:Agaricales / Q000737:chemistry* / Q000235:genetics; D004586:Electrophoresis; D012331:RNA, Fungal / Q000235:genetics / Q000302:isolation & purification*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013056:Spectrophotometry, Ultraviolet
chemical_list: D012331:RNA, Fungal