title: Identification of genes transcribed by Pasteurella multocida in rabbit livers through the selective capture of transcribed sequences.
abstract: Pasteurella multocida, a Gram-negative nonmotile coccobacillus, is the causative agent of fowl cholera in poultry, hemorrhagic septicemia in cattle, atropic rhinitis in swine, and snuffles in rabbits. The differentially expressed gene profile of P. multocida in infected rabbit livers was identified and compared with that from in vitro culture by selective capture of transcribed sequences. A total of 31 genes were identified, of which 28 encoded enzymes for amino acid biosynthesis and metabolism, intermediary metabolism, and energy metabolism, or proteins for regulatory adaptive responses, general microbial stress response, transport proteins, and secreted proteinases. Three were unknown, novel genes. Five genes representing different categories were chosen randomly and verified by real-time reverse transcriptase-polymerase chain reaction analysis. All were upregulated by P. multocida in infected rabbit livers, with changes ranging from 1.61- to 13.55-fold when compared with in vitro cultures. This study has identified genes of P. multocida that are upregulated during infection of rabbit livers when compared with in vitro growth conditions. The genes will provide a molecular basis for further study of the pathogenesis of P. multocida.
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
authors.forename: Dongchun
authors.initials: D
authors.lastname: Guo
authors.forename: Yan
authors.initials: Y
authors.lastname: Lu
authors.forename: Aiqin
authors.initials: A
authors.lastname: Zhang
authors.forename: Jiasen
authors.initials: J
authors.lastname: Liu
authors.forename: Dongwei
authors.initials: D
authors.lastname: Yuan
authors.forename: Qian
authors.initials: Q
authors.lastname: Jiang
authors.forename: Huan
authors.initials: H
authors.lastname: Lin
authors.forename: Changde
authors.initials: C
authors.lastname: Si
authors.forename: Liandong
authors.initials: L
authors.lastname: Qu
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D003470:Culture Media; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D008099:Liver / Q000382:microbiology*; D010326:Pasteurella Infections / Q000382:microbiology / Q000662:veterinary*; D016979:Pasteurella multocida / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity*; D011817:Rabbits; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D037521:Virulence Factors