title: Oceanibacterium hippocampi gen. nov., sp. nov., isolated from cutaneous mucus of wild seahorses (Hippocampus guttulatus).
abstract: A Gram-negative, aerobic, motile and slightly curved rod-shaped bacterium (BFLP-8(T)) was isolated from cutaneous mucus of wild long-snouted seahorses (Hippocampus guttulatus) captured in northwest Spain (Toralla, Galicia). Strain BFLP-8(T) grew at 10-35 °C and pH 5-9 (optimally at 25 °C and pH 7.0) and with 1-6 % (w/v) NaCl (optimally with 2 % NaCl). The predominant respiratory quinone (90 %) was ubiquinone with ten isoprene units (Q-10) and the major fatty acids identified were C(18:1)                         ω7c (54.8 % of the total), C(19:0) cyclo ω8c (11.6 %), C(16:0) (9.5 %), C(18:1) 2-OH (7.1 %) and C(16:1)                         ω11c (6.7 %). The G+C content of the DNA was 57.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BFLP-8(T) formed a distinct clade within the family Sneathiellaceae but is not specifically associated with any species in the family. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain BFLP-8(T) represents a novel species within a new genus, for which the name Oceanibacterium hippocampi gen. nov., sp. nov. is proposed. The type strain is BFLP-8(T) (=CECT 7691(T) = DSM 23444(T)).
authors.affiliation: Instituto de Investigaciones Marinas, Consejo Superior de Investigaciones Científicas, 36208, Vigo, Spain. jlbalcazar@icra.cat
authors.forename: José Luis
authors.initials: JL
authors.lastname: Balcázar
authors.forename: Miquel
authors.initials: M
authors.lastname: Planas
authors.forename: José
authors.initials: J
authors.lastname: Pintado
mesh_terms: D020561:Alphaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D000818:Animals; D001482:Base Composition; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009093:Mucus / Q000382:microbiology; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D023701:Smegmamorpha / Q000382:microbiology*; D012965:Sodium Chloride / Q000378:metabolism; D013030:Spain; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride