title: Molecular detection of rifabutin-susceptible Mycobacterium tuberculosis.
abstract: Rapid assays are still needed to detect rifabutin (RFB) susceptibility for proper tuberculosis treatment. To assess the use of the GenoType MTBDRplus assay and subsequent rpoB gene sequencing on detection of RFB susceptibility, we analyzed 800 multidrug-resistant Mycobacterium tuberculosis isolates, and 13% (104/800) were RFB susceptible. Of the 104 RFB-susceptible isolates, 71 (68.3%) isolates were rapidly identified using two molecular assays, while the remaining isolates could be determined using conventional drug-susceptibility testing according to the clinician's decision.
authors.affiliation: Reference Laboratory of Mycobacteriology, Research and Diagnostic Center, Centers for Disease Control, Taipei, Taiwan.
authors.forename: Huang-Yao
authors.initials: HY
authors.lastname: Chen
authors.forename: Ming-Chih
authors.initials: MC
authors.lastname: Yu
authors.forename: Wei-Lun
authors.initials: WL
authors.lastname: Huang
authors.forename: Mei-Hua
authors.initials: MH
authors.lastname: Wu
authors.forename: Yung-Lin
authors.initials: YL
authors.lastname: Chang
authors.forename: Chien-Rai
authors.initials: CR
authors.lastname: Che
authors.forename: Ruwen
authors.initials: R
authors.lastname: Jou
mesh_terms: D000995:Antitubercular Agents / Q000494:pharmacology*; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics; D017828:Rifabutin / Q000494:pharmacology*; D018088:Tuberculosis, Multidrug-Resistant / Q000382:microbiology*
chemical_list: D000995:Antitubercular Agents; D017828:Rifabutin; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit