title: Use of RapidChek® SELECT™ Salmonella to detect shedding of live attenuated Salmonella enterica serovar Typhi vaccine strains.
abstract: Identification of individuals shedding Salmonella enterica serovar Typhi in stool is imperative during clinical trial safety evaluations. Recovery of live attenuated S. Typhi vaccine strains can be difficult because the mutations necessary for safety in humans often compromise survival in stringent selective enrichment media. RapidChek® SELECT™ Salmonella is a highly sensitive detection method for S. enterica species which utilizes a bacteriophage cocktail designed to reduce the growth of competitor microbes in mildly selective enrichment medium. Detection of Salmonella is enhanced by means of a Salmonella-specific antibody strip targeted to lipopolysaccharide. The RapidChek® SELECT™ Salmonella method was compared to conventional enrichment and plating methods to determine the most sensitive method for detecting attenuated S. Typhi strains in human stool samples. Although traditional enrichment strategies were more sensitive to the presence of wild-type S. Typhi, RapidChek® SELECT™ Salmonella was superior at detecting attenuated strains of S. Typhi. Strains containing a wide variety of attenuating mutations were detected with equal sensitivity as the wild type by RapidChek® SELECT™ Salmonella. The presence of Vi capsule or mutations which affected O-antigen synthesis (Δpmi, ΔgalE) did not decrease the sensitivity of the RapidChek® SELECT™ Salmonella assay.
authors.affiliation: Center for Infectious Diseases and Vaccinology, The Biodesign Institute, Arizona State University, Tempe, AZ, USA. karen.brenneman@asu.edu
authors.forename: Karen E
authors.initials: KE
authors.lastname: Brenneman
authors.forename: Caitlin
authors.initials: C
authors.lastname: McDonald
authors.forename: Sandra M
authors.initials: SM
authors.lastname: Kelly-Aehle
authors.forename: Kenneth L
authors.initials: KL
authors.lastname: Roland
authors.forename: Roy
authors.initials: R
authors.lastname: Curtiss
mesh_terms: D057138:Bacterial Shedding; D001431:Bacteriological Techniques / Q000379:methods*; D001435:Bacteriophages / Q000254:growth & development; D006805:Human Experimentation; D006801:Humans; D012485:Salmonella typhi / Q000254:growth & development / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D014436:Typhoid-Paratyphoid Vaccines / Q000008:administration & dosage*; D014613:Vaccines, Attenuated / Q000008:administration & dosage
chemical_list: D014436:Typhoid-Paratyphoid Vaccines; D014613:Vaccines, Attenuated