title: Hemocompatible poly(NIPAm-MBA-AMPS) colloidal nanoparticles as carriers of anti-inflammatory cell penetrating peptides.
abstract: Anionic copolymer systems containing sulfated monomers have great potential for delivery of cationic therapeutics, but N-isopropylacrylamide (NIPAm) 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS) copolymer nanoparticles have seen limited characterization to date with regard to physical properties relevant to loading and release of therapeutics. Characterization of polymeric nanoparticles incorporating AMPS showed an increased size and decreased thermodynamic swelling ratios of AMPS containing particles as compared to NIPAm nanoparticles lacking AMPS. Particles with increasing AMPS addition showed an increased propensity for uniformity, intraparticle colloidal stability, and drug loading capacity. Peptide encapsulated in particles was shielded from peptide degradation in serum. Particles were shown not impede blood coagulation or to cause hemolysis. This study has demonstrated that AMPS incorporation into traditional NIPAm nanoparticles presents a tunable parameter for changing particle LCST, size, swelling ratio, ζ potential, and cationic peptide loading potential. This one-pot synthesis results in a thermosensitive anionic nanoparticle system that is a potentially useful platform to deliver cationic cell penetrating peptides.
authors.affiliation: Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana 47907, USA.
authors.forename: Rush L
authors.initials: RL
authors.lastname: Bartlett
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Medow
authors.forename: Alyssa
authors.initials: A
authors.lastname: Panitch
authors.forename: Brandon
authors.initials: B
authors.lastname: Seal
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D001672:Biocompatible Materials / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D001777:Blood Coagulation / Q000187:drug effects; D002417:Cattle; D057846:Cell-Penetrating Peptides / Q000737:chemistry* / Q000378:metabolism; D003102:Colloids / Q000737:chemistry / Q000378:metabolism; D004337:Drug Carriers / Q000737:chemistry* / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D010314:Partial Thromboplastin Time; D010316:Particle Size; D013499:Surface Properties; D013816:Thermodynamics
chemical_list: D000180:Acrylic Resins; D000894:Anti-Inflammatory Agents, Non-Steroidal; D001672:Biocompatible Materials; D057846:Cell-Penetrating Peptides; D003102:Colloids; D004337:Drug Carriers; C574752:poly(N-isopropylacrylamide-N,N'-methylenebisacrylamide-2-acrylamido-2-methyl-1-propanesulfonic acid)