title: Flow cytometric chromosome sorting in plants: the next generation.
abstract: Genome analysis in many plant species is hampered by large genome size and by sequence redundancy due to the presence of repetitive DNA and polyploidy. One solution is to reduce the sample complexity by dissecting the genomes to single chromosomes. This can be realized by flow cytometric sorting, which enables purification of chromosomes in large numbers. Coupling the chromosome sorting technology with next generation sequencing provides a targeted and cost effective way to tackle complex genomes. The methods outlined in this article describe a procedure for preparation of chromosomal DNA suitable for next-generation sequencing.
authors.affiliation: Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany, Sokolovská 6, CZ-77200 Olomouc, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Vrána
authors.forename: Hana
authors.initials: H
authors.lastname: Simková
authors.forename: Marie
authors.initials: M
authors.lastname: Kubaláková
authors.forename: Jarmila
authors.initials: J
authors.lastname: Cíhalíková
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Doležel
mesh_terms: D022202:Chromosomes, Artificial, Bacterial; D032461:Chromosomes, Plant / Q000648:ultrastructure*; D005434:Flow Cytometry / Q000379:methods; D059646:Genome Size; D018745:Genome, Plant; D018525:Germination / Q000235:genetics; D059014:High-Throughput Nucleotide Sequencing; D001467:Hordeum / Q000166:cytology / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D008677:Metaphase / Q000235:genetics; D008856:Microscopy, Fluorescence; D011123:Polyploidy; D012434:Secale / Q000166:cytology / Q000235:genetics*; D012639:Seeds / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014908:Triticum / Q000166:cytology / Q000235:genetics*