title: Ion-exchange centrifugal partition chromatography: a methodological approach for peptide separation.
abstract: This article presents the scope and optimization strategies employed in ion-exchange centrifugal partition chromatography (IXCPC). Both the weak and the strong modes were used to separate the constituents of a model mixture of dipeptides. Thus, the combined use of the quaternary biphasic solvent system, methyl-tert-butylether/acetonitrile/n-butanol/water (2:1:2:5, v/v) in the descending mode, of the lipophilic di(2-ethylhexyl)phosphoric acid (DEHPA) cation-exchanger, and of two displacers: calcium chloride and hydrochloric acid, has proven to be efficient for the preparative separation of the model mixture of five dipeptides (GG, GY, AY, LV and LY, in the order they were collected). The separation was optimized by splitting the stationary phase into two sections that differed by their triethylamine concentration. Moreover, the chemical nature of the exchanger/analyte entities that were involved in the chromatographic process was determined by (31)P and (1)H DOSY NMR experiments.
authors.affiliation: ICMR UMR CNRS 6229, IFR 53 Interactions Cellules Microenvironnement, Université de Reims Champagne-Ardenne, Bât. 18, Moulin de la Housse, B.P. 1039, 51687 Reims Cedex 2, France.
authors.forename: Leslie
authors.initials: L
authors.lastname: Boudesocque
authors.forename: Pedro
authors.initials: P
authors.lastname: Lameiras
authors.forename: Nassima
authors.initials: N
authors.lastname: Amarouche
authors.forename: Matthieu
authors.initials: M
authors.lastname: Giraud
authors.forename: Francesca
authors.initials: F
authors.lastname: Quattrini
authors.forename: John Mc
authors.initials: JM
authors.lastname: Garrity
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Nuzillard
authors.forename: Jean-Hugues
authors.initials: JH
authors.lastname: Renault
mesh_terms: D002498:Centrifugation / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D004151:Dipeptides / Q000737:chemistry / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D010755:Organophosphates / Q000737:chemistry
chemical_list: D004151:Dipeptides; D010755:Organophosphates; C007377:di-2-(ethylhexyl)phosphoric acid