title: Recognition of copper and hydrogen sulfide in vitro using a fluorescein derivative indicator.
abstract: We report the development of a fluorescein-based chemosensor (L1) for monitoring ions or micromolecules (H(2)S). Copper ions are known to be toxic at high concentrations and hydrogen sulfide induces various problems. Herein we develop a simple method for detecting Cu(II) and H(2)S with high selectivity and sensitivity. The chemosensor L1 displays on-off-on type fluorescence change with alternately added Cu(II) and H(2)S to the media along with reversible forming-separating of the complex. The potential biomedical relevance of the chemical mechanisms involved in the detection of L1 is described.
authors.affiliation: Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province and College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, PR China.
authors.forename: Fengping
authors.initials: F
authors.lastname: Hou
authors.forename: Ju
authors.initials: J
authors.lastname: Cheng
authors.forename: Pinxian
authors.initials: P
authors.lastname: Xi
authors.forename: Fengjuan
authors.initials: F
authors.lastname: Chen
authors.forename: Liang
authors.initials: L
authors.lastname: Huang
authors.forename: Gouqiang
authors.initials: G
authors.lastname: Xie
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Shi
authors.forename: Hongyan
authors.initials: H
authors.lastname: Liu
authors.forename: Decheng
authors.initials: D
authors.lastname: Bai
authors.forename: Zhengzhi
authors.initials: Z
authors.lastname: Zeng
mesh_terms: D000818:Animals; D003300:Copper / Q000032:analysis* / Q000737:chemistry; D018360:Crystallography, X-Ray; D019793:Fluorescein / Q000032:analysis / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D006862:Hydrogen Sulfide / Q000032:analysis* / Q000737:chemistry; D051379:Mice; D008958:Models, Molecular; D008968:Molecular Conformation; D057054:Molecular Imaging
chemical_list: D005456:Fluorescent Dyes; D003300:Copper; D019793:Fluorescein; D006862:Hydrogen Sulfide