title: Silanimonas mangrovi sp. nov., a member of the family Xanthomonadaceae isolated from mangrove sediment, and emended description of the genus Silanimonas.
abstract: A novel Gram-negative, rod-shaped, motile bacterium, designated strain AK13(T), was isolated from a sediment sample collected from mangrove of Namkhana, Sunderbans, West Bengal, India. Strain AK13(T) was positive for oxidase, DNase and lipase activities and negative for catalase, gelatinase, ornithine decarboxylase, lysine decarboxylase, nitrate reductase, aesculinase and urease activities. The fatty acids were dominated by iso-C(11 : 0), iso-C(11 : 0) 3-OH, iso-C(15 : 0), iso-C(16 : 0), iso-C(17 : 1)ω9c and summed feature 3 (C(16 : 1)ω7c and/or iso-C(15 : 0) 2-OH). Strain AK13(T) contained Q-8 as the major respiratory quinone and diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylserine, two unidentified aminolipids, one unidentified glycolipid and one unidentified lipid as the polar lipids. The DNA G+C content of strain AK13(T) was 55.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the type strain of Silanimonas lenta, of the family Xanthomonadaceae (phylum Proteobacteria), was the closest neighbour of strain AK13(T), with 95.2 % sequence similarity. Other members of the family showed sequence similarities <94.4 %. Based on the phenotypic characteristics and phylogenetic inference, strain AK13(T) is proposed as a member of a novel species of the genus Silanimonas, Silanimonas mangrovi sp. nov.; the type strain is AK13(T) (= MTCC 11082(T) = DSM 24914(T)). An emended description of the genus Silanimonas is also provided.
authors.affiliation: CSIR - National Institute of Oceanography, Regional Centre, PB No. 1913, Dr Salim Ali Road, Kochi 682018 (Kerala), India.
authors.forename: T N R
authors.initials: TNR
authors.lastname: Srinivas
authors.affiliation: Microbial Type Culture Collection and Gene bank, CSIR - Institute of Microbial Technology, Sector 39A, Chandigarh 160036, India.
authors.forename: T B
authors.initials: TB
authors.lastname: Kailash
authors.affiliation: Microbial Type Culture Collection and Gene bank, CSIR - Institute of Microbial Technology, Sector 39A, Chandigarh 160036, India.
authors.forename: Pinnaka
authors.initials: P
authors.lastname: Anil Kumar
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology; D007194:India; D008969:Molecular Sequence Data; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014871:Water Microbiology; D053833:Wetlands; D044166:Xanthomonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S