title: Biological response to porcine xenograft implants:  an experimental study in rabbits.
abstract: OBJECTIVE
  The aim of this study was to evaluate the effect of a new porcine biomaterial and collagen paste in 20 New Zealand rabbits.
  
  
  METHODS
  Forty implants using a porcine xenograft made up of 80% corticocancellous collagenated bone particles of ≤300 μm in size were placed in the proximal metaphyseal area of both tibiae. Four periods of time were formed: 1h, 5, 8, and 15 months. After implantation, an anteroposterior and lateral radiological study was carried out. Samples were sectioned at 5 μm and stained using hematoxylin-eosin, Masson's trichromic, and Gordon-Switt reticulin stains.
  
  
  RESULTS
  These results confirmed the biocompatibility of this porcine biomaterial-collagen paste; only a few, occasional macrophages and scattered lymphocytes were observed. No fibrosis was observed between the implants and the bone. Moreover, the material was osteoconductive acting as a "scaffold" for bone cells, and there was a progressive increase in bone growth in and around the implants.
  
  
  CONCLUSIONS
  This new porcine biomaterial-collagen paste seemed to be biocompatible, bioresorbable, and osteoconductive.
authors.affiliation: General Dentistry, Faculty of Medicine and Dentistry, University of Murcia, Murcia, Spain. josecalvog@gmail.com
authors.forename: José Luis
authors.initials: JL
authors.lastname: Calvo-Guirado
authors.forename: Gerardo
authors.initials: G
authors.lastname: Gómez-Moreno
authors.forename: Javier
authors.initials: J
authors.lastname: Guardia
authors.forename: Antonio
authors.initials: A
authors.lastname: Ortiz-Ruiz
authors.forename: Adriano
authors.initials: A
authors.lastname: Piatelli
authors.forename: Antonio
authors.initials: A
authors.lastname: Barone
authors.forename: José María
authors.initials: JM
authors.lastname: Martínez-González
authors.forename: Luis
authors.initials: L
authors.lastname: Meseguer-Olmo
authors.forename: Laura
authors.initials: L
authors.lastname: López-Marí
authors.forename: Cristina Barona
authors.initials: CB
authors.lastname: Dorado
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use*; D015519:Bone Density / Q000502:physiology; D001853:Bone Marrow / Q000473:pathology; D018786:Bone Substitutes / Q000627:therapeutic use; D016025:Bone Transplantation / Q000473:pathology*; D002113:Calcification, Physiologic / Q000502:physiology; D024042:Collagen Type I / Q000627:therapeutic use*; D004396:Coloring Agents; D005347:Fibroblasts / Q000473:pathology; D005456:Fluorescent Dyes; D008214:Lymphocytes / Q000473:pathology; D008264:Macrophages / Q000473:pathology; D010006:Osteoblasts / Q000473:pathology; D010012:Osteogenesis / Q000502:physiology; D011817:Rabbits; D013552:Swine; D013977:Tibia / Q000473:pathology / Q000601:surgery; D013997:Time Factors; D054457:Tissue Scaffolds; D014183:Transplantation, Heterologous
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D024042:Collagen Type I; D004396:Coloring Agents; D005456:Fluorescent Dyes