title: Bacterial diterpene synthases: new opportunities for mechanistic enzymology and engineered biosynthesis.
abstract: Diterpenoid biosynthesis has been extensively studied in plants and fungi, yet cloning and engineering diterpenoid pathways in these organisms remain challenging. Bacteria are emerging as prolific producers of diterpenoid natural products, and bacterial diterpene synthases are poised to make significant contributions to our understanding of terpenoid biosynthesis. Here we will first survey diterpenoid natural products of bacterial origin and briefly review their biosynthesis with emphasis on diterpene synthases (DTSs) that channel geranylgeranyl diphosphate to various diterpenoid scaffolds. We will then highlight differences of DTSs of bacterial and higher organism origins and discuss the challenges in discovering novel bacterial DTSs. We will conclude by discussing new opportunities for DTS mechanistic enzymology and applications of bacterial DTS in biocatalysis and metabolic pathway engineering.
authors.affiliation: Microbiology Doctoral Training Program, University of Wisconsin-Madison, Madison, WI 53705, USA.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Smanski
authors.forename: Ryan M
authors.initials: RM
authors.lastname: Peterson
authors.forename: Sheng-Xiong
authors.initials: SX
authors.lastname: Huang
authors.forename: Ben
authors.initials: B
authors.lastname: Shen
mesh_terms: D001419:Bacteria / Q000201:enzymology*; D001688:Biological Products / Q000737:chemistry / Q000378:metabolism; D053898:Biosynthetic Pathways; D004224:Diterpenes / Q000737:chemistry / Q000378:metabolism*; D010802:Phylogeny; D015202:Protein Engineering
chemical_list: D001688:Biological Products; D004224:Diterpenes