title: Recovery of leptospires from miniature pigs experimentally infected with Leptospira interrogans serovar Manilae strain UP-MMC under immunosuppressive conditions by dexamethasone.
abstract: Leptospira interrogans serovar Manilae strain UP-MMC was inoculated into miniature pigs to assess its pathogenicity. Leptospires were recovered from the whole blood, kidneys, and livers in the acute phase without showing any clinical signs. Under immunosuppressive conditions by dexamethasone, leptospires were recovered from the kidneys and their genes were detected from the urine in the chronic phase. These results indicate that leptospires persisted in the kidneys until the chronic phase, and excretion of leptospires in the urine was enhanced under immunosuppressive conditions, resulting in horizontal transmission among pigs on farms.
authors.affiliation: Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Sakoda
authors.forename: Michiko
authors.initials: M
authors.lastname: Naito
authors.forename: Mutsumi
authors.initials: M
authors.lastname: Ito
authors.forename: Yuki
authors.initials: Y
authors.lastname: Ito
authors.forename: Norikazu
authors.initials: N
authors.lastname: Isoda
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Tanaka
authors.forename: Takashi
authors.initials: T
authors.lastname: Umemura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kida
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001831:Body Temperature; D017931:DNA Primers / Q000235:genetics; D003907:Dexamethasone / Q000378:metabolism*; D005260:Female; D007165:Immunosuppression Therapy; D007668:Kidney / Q000382:microbiology; D007921:Leptospira interrogans / Q000472:pathogenicity*; D008099:Liver / Q000382:microbiology; D016133:Polymerase Chain Reaction; D013552:Swine; D013556:Swine, Miniature / Q000382:microbiology*; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; C027193:leptospirin; D003907:Dexamethasone