title: Mxi1 influences cyst formation in three-dimensional cell culture.
abstract: Cyst formation is a major characteristic of ADPKD and is caused by the abnormal proliferation of epithelial cells. Renal cyst formation disrupts renal function and induces diverse complications. The mechanism of cyst formation is unclear. mIMCD-3 cells were established to develop simple epithelial cell cysts in 3-D culture. We confirmed previously that Mxi1 plays a role in cyst formation in Mxi1-deficient mice. Cysts in Mxi1 transfectanted cells were showed by collagen or mebiol gels in 3-D cell culture system. Causative genes of ADPKD were measured by q RT-PCR. Herein, Mxi1 transfectants rarely formed a simple epithelial cyst and induced cell death. Overexpression of Mxi1 resulted in a decrease in the PKD1, PKD2 and c-myc mRNA relating to the pathway of cyst formation. These data indicate that Mxi1 influences cyst formation of mIMCD-3 cells in 3-D culture and that Mxi1 may control the mechanism of renal cyst formation.
authors.affiliation: Department of Biological Science, Sookmyung Women's University, Seoul, Korea.
authors.forename: Yeon Joo
authors.initials: YJ
authors.lastname: Yook
authors.forename: Kyung Hyun
authors.initials: KH
authors.lastname: Yoo
authors.forename: Seon Ah
authors.initials: SA
authors.lastname: Song
authors.forename: Min Ji
authors.initials: MJ
authors.lastname: Seo
authors.forename: Je Yeong
authors.initials: JY
authors.lastname: Ko
authors.forename: Bo Hye
authors.initials: BH
authors.lastname: Kim
authors.forename: Eun-Ji
authors.initials: EJ
authors.lastname: Lee
authors.forename: Eunsun
authors.initials: E
authors.lastname: Chang
authors.forename: Yu Mi
authors.initials: YM
authors.lastname: Woo
authors.forename: Jong Hoon
authors.initials: JH
authors.lastname: Park
mesh_terms: D000818:Animals; D017209:Apoptosis; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism*; D018929:Cell Culture Techniques; D002470:Cell Survival; D002478:Cells, Cultured; D003560:Cysts / Q000378:metabolism*; D051379:Mice; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D025521:Tumor Suppressor Proteins; C512788:Mxi1 protein, mouse