title: Changes in cellular response to the damage induced in PC-3 prostate cancer cells by proton microbeam irradiation.
abstract: The aim of this research was to find out whether the passage number effect may influence on the PC-3 cells (the human prostate cancer line derived from bone metastases) response to proton radiation. 2 MeV horizontally focused proton microbeam was used as a radiation source. The cells were treated with a counted number of H(+) ions (50-8000) corresponding to doses of 1.3-209 Gy/cell. For comparison, cell death was also induced by UVC radiation. All cells were stained with Hoechst 33342 and propidium iodide and visualized under a fluorescence microscope. Necrosis was observed at: a) 8000 protons per cell (corresponding to ∼209 Gy/cell) after 2-4 passages, b) 3200 protons per cell (corresponding to ∼84 Gy/cell) for cells after 11-14 passages and c) only 800 protons per cell (corresponding to ∼2 Gy/cell ) after 47-50 passages. Apoptosis was efficiently induced, by protons, only in cells after 50 passages. The results showed that the laboratory conditions affected cellular response of PC-3 cell line to the proton irradiation. The cellular response to the radiation treatment strongly depends on number of passages.
authors.affiliation: The Henryk Niewodniczański Institute of Nuclear Physics, Polish Academy of Sciences, Kraków, Poland.
authors.forename: Ewelina W
authors.initials: EW
authors.lastname: Lipiec
authors.forename: Anna
authors.initials: A
authors.lastname: Wiecheć
authors.forename: Joanna
authors.initials: J
authors.lastname: Dulińska-Litewka
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Kubica
authors.forename: Janusz
authors.initials: J
authors.lastname: Lekki
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Stachura
authors.forename: Joanna
authors.initials: J
authors.lastname: Wiltowska-Zuber
authors.forename: Wojciech M
authors.initials: WM
authors.lastname: Kwiatek
mesh_terms: D017209:Apoptosis / Q000528:radiation effects*; D001859:Bone Neoplasms / Q000473:pathology* / Q000532:radiotherapy / Q000556:secondary*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000528:radiation effects; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000473:pathology* / Q000503:physiopathology* / Q000532:radiotherapy; D011522:Protons
chemical_list: D011522:Protons