title: 4(R/S)-Guanidinylprolyl collagen peptides: on-resin synthesis, complexation with plasmid DNA, and the role of peptides in enhancement of transfection.
abstract: Chimeric collagen peptides containing cationic 4(R/S)-guanidinylproline are synthesized by in situ amidinylation of 4(R/S)-aminoproline residues. These peptides uniquely enhance the transfection efficiency of GFP-encoded plasmid DNA (pRmHa3-GFP) into cells through efficient DNA condensation resulting from nonspecific electrostatic interactions of cationic guanidino groups and localize in subcytoplasmic organelles.
authors.affiliation: Organic Chemistry Division, National Chemical Laboratory, Pune 411008, India.
authors.forename: Manaswini
authors.initials: M
authors.lastname: Nanda
authors.forename: Krishna N
authors.initials: KN
authors.lastname: Ganesh
mesh_terms: D003094:Collagen / Q000737:chemistry* / Q000378:metabolism; D004247:DNA / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D018014:Gene Transfer Techniques; D006146:Guanidines / Q000737:chemistry; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D010957:Plasmids / Q000737:chemistry* / Q000378:metabolism; D012117:Resins, Synthetic / Q000737:chemistry*; D014162:Transfection
chemical_list: D006146:Guanidines; D010455:Peptides; D012117:Resins, Synthetic; D003094:Collagen; D004247:DNA