title: Comparison of neutralizing and hemagglutination-inhibiting antibody responses for evaluating the seasonal influenza vaccine.
abstract: Evaluation of the efficacy of influenza vaccines is essential for vaccine development. This study evaluated the neutralizing and hemagglutination-inhibition antibody response in subjects receiving the 2006-07 and 2007-08 seasonal influenza vaccines. ELISA-based microneutralization demonstrated a greater mean-fold increase and seroconversion rate than the hemagglutination-inhibition assay. The increase in the antibody titers against influenza H1 were higher than those against influenza H3 and influenza B, indicating that the H1 vaccine strain in the 2006-07 and 2007-08 seasons was more immunogenic. These data suggest that the neutralizing antibody response is a better measurement of influenza vaccine efficacy.
authors.affiliation: Department of Medical Laboratory Science and Biotechnology, National Cheng Kung University, Tainan 701, Taiwan.
authors.forename: Li-Wei
authors.initials: LW
authors.lastname: Cheng
authors.forename: Sheng-Wen
authors.initials: SW
authors.lastname: Huang
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Huang
authors.forename: Luan-Yin
authors.initials: LY
authors.lastname: Chang
authors.forename: Pei-Lan
authors.initials: PL
authors.lastname: Shao
authors.forename: David
authors.initials: D
authors.lastname: Kiang
authors.forename: Jen-Ren
authors.initials: JR
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006386:Hemagglutination Tests / Q000379:methods*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009500:Neutralization Tests / Q000379:methods*; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D007252:Influenza Vaccines