title: [Heterogeneity of hepatitis B virus and diagnostic potential of modern test systems for the detection of HBsAg].
abstract: OBJECTIVE
  Study heterogeneity ofhepatitis B virus in adult patients with chronic hepatitis B and determination of diagnostic potential of modern test systems with the detection of HBsAg with amino acid substitutions in the main hydrophilic region (MHR).
  
  
  METHODS
  In 27 hepatitis B virus samples isolated from patients with chronic hepatitis B virus infection living in Vladimir, nucleotide sequence ofgenome region corresponding to preS1/preS2/S genes was determined.
  
  
  RESULTS
  In all of the 27 isolates genotype D virus presented by 3 subgenotypes D1, D2, D3 was detected in 18%, 26% and 56% respectively. Based on the distribution of nucleotide substitutions in the compared functional regions of hepatitis B virus (virus entry into the cell coding site (2875 - 2991 n.b.), pre-S2/S promoter region (2994 - 3171 n.b.), 5'-end pre-S2 and S-genes sequences (3172 - 154 n.b. and 155-455 n.b.), MHR (455 - 635 n.b.) and 3'-end S-gene sequence (636 - 835 n.b.), substitutions are mostly concentrated in the promoter region of the S2/S-genes (30.8%). HBsAg serotypes were determined in 24 of 27 cases by using the predicted amino acid sequence, and in 17 cases HBsAg belonged to ayw2 (71%) serotype and in 7 cases - to ayw3 serotype (29%). Amino acid substitutions G145A, M133I, S132T localized in the main hydrophilic region and P217L, S207N, V184A localized in the C-end of the protein C that are connected with diagnostic and vaccine escape were identified in 5 isolates.
  
  
  CONCLUSIONS
  Diagnostic potential of test systems with the detection of HBsAg with known amino acid sequence of the MHR region were studied. Approximately equal potential of 6 test systems to detect HBsAg with amino acid substitutions G145A, M133I and S132T localized in the MHR region were shown.
authors.forename: S N
authors.initials: SN
authors.lastname: Kuzin
authors.forename: E E
authors.initials: EE
authors.lastname: Zabotina
authors.forename: N N
authors.initials: NN
authors.lastname: Zabelin
authors.forename: E N
authors.initials: EN
authors.lastname: Kudriavtseva
authors.forename: E I
authors.initials: EI
authors.lastname: Samokhvalov
authors.forename: O V
authors.initials: OV
authors.lastname: Borisova
authors.forename: T A
authors.initials: TA
authors.lastname: Manina
authors.forename: E V
authors.initials: EV
authors.lastname: Lisitsina
authors.forename: L E
authors.initials: LE
authors.lastname: Kuzina
authors.forename: N A
authors.initials: NA
authors.lastname: Malyshev
authors.forename: V F
authors.initials: VF
authors.lastname: Lavrov
mesh_terms: D000328:Adult; D019943:Amino Acid Substitution / Q000276:immunology; D004279:DNA, Viral / Q000032:analysis* / Q000096:biosynthesis; D005260:Female; D018740:Genetic Heterogeneity; D005838:Genotype; D006510:Hepatitis B Antibodies / Q000032:analysis / Q000276:immunology; D006514:Hepatitis B Surface Antigens / Q000145:classification / Q000235:genetics* / Q000276:immunology; D006515:Hepatitis B virus / Q000145:classification / Q000235:genetics* / Q000276:immunology; D019694:Hepatitis B, Chronic / Q000175:diagnosis* / Q000276:immunology; D006801:Humans; D007118:Immunoassay; D008297:Male; D008875:Middle Aged; D009154:Mutation; D016366:Open Reading Frames / Q000276:immunology; D011401:Promoter Regions, Genetic / Q000276:immunology; D011498:Protein Precursors / Q000145:classification / Q000235:genetics* / Q000276:immunology; D017434:Protein Structure, Tertiary; D012426:Russia; D012680:Sensitivity and Specificity
chemical_list: D004279:DNA, Viral; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D011498:Protein Precursors; C050806:presurface protein 1, hepatitis B surface antigen; C050807:presurface protein 2, hepatitis B surface antigen