title: Expression and purification of bioactive high-purity human S100A6 in Escherichia coli.
abstract: S100A6, as a member of S100 protein family, have biological functions in cell proliferation, differentiation, morphology, cytoskeletal organization and apoptosis. In the last three decades, S100A6 has been caught more and more attention. Here, we introduced a simple and efficient method for producing high-purity recombinant human S100A6 from Escherichia coli culture with low level of endotoxin. We further demonstrated its biological activities for triggering SH-SY5Y cells apoptosis in vitro. These results can facilitate the study of physiological and pathological roles of S100A6 and other members of S100 family proteins.
authors.affiliation: Shanghai Municipality Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.
authors.forename: Honglin
authors.initials: H
authors.lastname: He
authors.forename: Tingxu
authors.initials: T
authors.lastname: Yang
authors.forename: Shixiang
authors.initials: S
authors.lastname: Jia
authors.forename: Ruliang
authors.initials: R
authors.lastname: Zhang
authors.forename: Ping
authors.initials: P
authors.lastname: Tu
authors.forename: Jin
authors.initials: J
authors.lastname: Gao
authors.forename: Yunsheng
authors.initials: Y
authors.lastname: Yuan
authors.forename: Wei
authors.initials: W
authors.lastname: Han
authors.forename: Yan
authors.initials: Y
authors.lastname: Yu
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D001483:Base Sequence; D018797:Cell Cycle Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D045744:Cell Line, Tumor; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D018797:Cell Cycle Proteins; D011994:Recombinant Proteins; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins; C055351:S100A6 protein, human