title: Micropropagation, antinociceptive and antioxidant activities of extracts of Verbena litoralis Kunth (Verbenaceae).
abstract: This work describes an efficient micropropagation protocol for Verbena litoralis and the study of the antinociceptive and antioxidant activities in extracts of this species. For the establishment in vitro, surface-sterilization procedures and PVPP showed high efficiency in fungal-bacterial contamination and phenol oxidation controls. Nodal segments cultivation in MS medium supplemented with 6-benzyladenine (7.5 µM)/α-naphthaleneacetic acid (NAA; 0.005 µM) induced multiple shoots. Elongated shoots were rooted with IAA (0.2 µM). Acclimatization rates were elevated and the plants showed the typical features of this species. The hexanic fraction (HF) of powdered leaves presented a radical scavenging activity with IC(50) = 169.3 µg mL(-1). HF showed a non-dose dependent analgesic activity in the writhing test; its antinociceptive activity in the hot plate test was restricted to 500 mg kg(-1), which is the highest dose. The results of this study showed the potential of tissue culture on conservation and large scale multiplication and confirmed the traditional folk medicine use of V. litoralis.
authors.affiliation: Instituto de Ciências Biológicas, Departamento de Botânica, Laboratório de Fisiologia Vegetal, Universidade Federal de Juiz de Fora, MG, Brasil.
authors.forename: Virgínia F
authors.initials: VF
authors.lastname: Braga
authors.forename: Giselle C
authors.initials: GC
authors.lastname: Mendes
authors.forename: Raphael T R
authors.initials: RT
authors.lastname: Oliveira
authors.forename: Carla Q G
authors.initials: CQ
authors.lastname: Soares
authors.forename: Cristiano F
authors.initials: CF
authors.lastname: Resende
authors.forename: Leandro C
authors.initials: LC
authors.lastname: Pinto
authors.forename: Reinaldo de
authors.initials: Rd
authors.lastname: Santana
authors.forename: Lyderson F
authors.initials: LF
authors.lastname: Viccini
authors.forename: Nádia R B
authors.initials: NR
authors.lastname: Raposo
authors.forename: Paulo H P
authors.initials: PH
authors.lastname: Peixoto
mesh_terms: D000064:Acclimatization; D000700:Analgesics / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D008297:Male; D051379:Mice; D010147:Pain Measurement / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots / Q000254:growth & development; D018520:Plant Shoots / Q000254:growth & development; D013997:Time Factors; D032412:Verbena / Q000737:chemistry* / Q000254:growth & development*
chemical_list: D000700:Analgesics; D000975:Antioxidants; D010936:Plant Extracts