title: [Responses of pancreatic cancer cells to stimulations by nerve growth factor and the role of Trk-A expression].
abstract: OBJECTIVE
  To study the responses of different pancreatic cancer cells to stimulations by nerve growth factor (NGF) and explore the role of Trk-A in such responses.
  
  
  METHODS
  Five pancreatic cancer cell lines (MIA-PaCa-2, PANC-1, SW-1990, AsPC-1, and BxPC-3) were exposed to different concentrations of NGF (0, 4, 20, 100, and 500 ng/ml). MTT and Matrigel invasion method were used to observe the changes in the cell proliferation and invasion ability. Trk-A expression in these cells was detected by PCR and Western blotting, and the relations of Trk-A expression to the cell proliferative and invasive abilities following NGF treatment were analyzed.
  
  
  RESULTS
  NGF at 100 ng/ml most obviously stimulated the cell proliferation, and PANC-1 cells showed the highest while AsPC-1 cells showed the least sensitivity to 100 ng/ml NGF stimulation. Matrigel invasion test showed that NGF enhanced the invasiveness of PANC-1 and MIA-PaCa-2 cells but produced only limited effect on AsPC-1 cells; the effect of NGF was completely inhibited by the Trk-A inhibitor CEP701. The expression levels of Trk-A mRNA and protein were the highest in PANC-1 cells and the lowest in AsPC-1 cells.
  
  
  CONCLUSIONS
  NGF can enhance the proliferation and invasiveness of pancreatic cancer cells, and this effect is possibly mediated by Trk-A protein.
authors.affiliation: Department of Surgical Oncology, First Affiliated Hosptial, Xi'an Jiaotong University College of Medicine, Xi'an 710061, China. diaomei310@gmail.com
authors.forename: Dong-Mei
authors.initials: DM
authors.lastname: Diao
authors.forename: Yong-Chun
authors.initials: YC
authors.lastname: Song
authors.forename: Ni
authors.initials: N
authors.lastname: Hou
authors.forename: Hai-Fei
authors.initials: HF
authors.lastname: Xu
authors.forename: Jian-Guang
authors.initials: JG
authors.lastname: Wang
authors.forename: Cheng-Xue
authors.initials: CX
authors.lastname: Dang
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D009361:Neoplasm Invasiveness; D020932:Nerve Growth Factor / Q000494:pharmacology*; D010190:Pancreatic Neoplasms / Q000378:metabolism* / Q000473:pathology*; D020917:Receptor, trkA / Q000235:genetics / Q000378:metabolism*
chemical_list: D020932:Nerve Growth Factor; D020917:Receptor, trkA