title: [Feasibility of cartilage link protein of hyaluronic acid for defining radiotherapeutic target volume in a mouse model of lung tumor].
abstract: OBJECTIVE
  To investigate the feasibility of using cartilage link protein of hyaluronic acid (HA-CLP) for defining the tumor boundary in a mouse model of lung carcinoma.
  
  
  METHODS
  Lung carcinoma was induced in KM mice by chemical carcinogenesis. HA-CLP separated from bovine cartilage and purified by affinity chromatography was labeled with (125)I for autoradiography. Immunohistochemical analysis and Western blotting were used to examine the efficiency of HA-CLP in defining the boundaries of the lung tumors.
  
  
  RESULTS
  With autoradiography, the clearest image of lung cancer was obtained at 2 h. With immunohistochemical method, the tumor boundary was the most clearly displayed at 2 h when the strongest signals of HA-CLP was detected; Western blotting also showed the clearest bands of HA-CLP at 2 h.
  
  
  CONCLUSIONS
  HA-CLP has the immunogenicity of HABP, and can efficiently indicate lung tumor boundary in autoradiography and immunohistochemistry.
authors.affiliation: Department of Experimental Nuclear Medicine, Guangzhou Medical College, Guangzhou 510182, China.
authors.forename: Zhi-Xin
authors.initials: ZX
authors.lastname: Liang
authors.forename: Yong-Gang
authors.initials: YG
authors.lastname: Qiang
authors.forename: Yong-Hua
authors.initials: YH
authors.lastname: Liao
mesh_terms: D000818:Animals; D001345:Autoradiography / Q000379:methods; D016326:Extracellular Matrix Proteins / Q000378:metabolism / Q000494:pharmacology*; D005260:Female; D006820:Hyaluronic Acid / Q000378:metabolism; D007150:Immunohistochemistry; D007457:Iodine Radioisotopes; D008175:Lung Neoplasms / Q000532:radiotherapy*; D008297:Male; D051379:Mice; D011509:Proteoglycans / Q000378:metabolism / Q000494:pharmacology*; D061089:Radiotherapy, Image-Guided / Q000379:methods*; D000099249:Proteoglycan Link Protein
chemical_list: D016326:Extracellular Matrix Proteins; D006820:Hyaluronic Acid; D007457:Iodine Radioisotopes; D011509:Proteoglycans; D000099249:Proteoglycan Link Protein