title: [Antitumor immune response of MHC class I chain-related gene A modified oral squamous cell carcinoma vaccine: an experimental study in mice].
abstract: OBJECTIVE
  To investigate the vaccine potency of MHC class I chain-related gene A (MICA) modified oral squamous cell carcinoma cells.
  
  
  METHODS
  Oral squamous cell carcinoma Tb cells transfected with eukaryotic expression vector pEGFP-N1-MICA and overexpressing MICA protein were inactivated by 120Gy irradiation and vaccinated human peripheral blood leucocytes reconstituted SCID (Hu-PBL/SCID)mice via intra-peritoneal injection, and the non-transfected or blank vector transfected Tb cells were used as the controls. The inhibition effect on tumorigenicity of subcutaneously challenged Tb cells in vaccinated Hu-PBL/SCID mice was detected.The expression of NKG2D and the cytotoxicity in vitro to Tb cells of peripheral blood mononuclear cells (PBMCs) and spleen cells were measured by flow cytometry and lactate dehydrogenase (LDH) release assay. SPSS16.0 software package was used for statistical analysis.
  
  
  RESULTS
  MICA gene modified Tb tumor vaccine resulted in remarkable loss of tumor size and tumor weight in vaccinated Hu-PBL/SCID mice. Flow cytometry and lactate dehydrogenase (LDH) release assay showed MICA gene modified Tb tumor vaccine up-regulated the expression of NKG2D on PBMC and spleen cells and enhanced the cytotoxicity to tumor cells. Significant difference was found between MICA-transfected vaccine and non-transfected and blank vector-transfected vaccine (P<0.05).
  
  
  CONCLUSIONS
  MICA gene modified oral squamous cell carcinoma vaccine can enhance the ability of antitumor immune response,and MICA may be considered as a promising immunotherapy target of oral squamous cell carcinoma.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Xiangya Hospital. Changsha, China. lichao79568211@yahoo.cn
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.forename: Fang-qiong
authors.initials: FQ
authors.lastname: Shi
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.forename: Dan
authors.initials: D
authors.lastname: Yang
authors.forename: Xin-chun
authors.initials: XC
authors.lastname: Jian
authors.forename: Can-hua
authors.initials: CH
authors.lastname: Jiang
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell; D005822:Genetic Vectors; D015395:Histocompatibility Antigens Class I; D006801:Humans; D007963:Leukocytes, Mononuclear; D051379:Mice; D016513:Mice, SCID; D009062:Mouth Neoplasms; D014162:Transfection; D014612:Vaccines
chemical_list: D015395:Histocompatibility Antigens Class I; C099187:MHC class I-related chain A; D014612:Vaccines