title: [Effect of TSA and VPA treatment on long-tailed macaque (Macaca fascicularis)-pig interspecies somatic cell nuclear transfer].
abstract: Long-tailed macaque-pig interspecies somatic cell nuclear transfer (iSCNT) is beneficial to yield embryonic stem cells from iSCNT embryos with similar genetic background as human, which can be used as materials for medical and basic research. The primary objective of this study was to investigate the effects of concentrations and treatment duration of two histone deacetylase inhibitors-Trichostatin A (TSA) and Valproic acid (VPA) and two different embryo culture media (PZM-3 and HECM-10) on the in vitro development of iSCNT embryos. The results suggested that when PZM-3 was used as the embryo culture medium, the blastocyst rate of 10 nmol/L TSA treatment for 48 h was significantly higher than the control group (22.78% vs 9.86%, P< 0.05). However, neither in PZM-3 nor in HECM-10, 2-10 mmol/L VPA treatment did not increase the in vitro developmental potential of iSCNT embryos. It was concluded that TSA treatment could enhance the in vitro developmental potential of long-tailed macaque-pig iSCNT embryos.
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Animal Reproduction Institute, Guangxi University, Nanning 530004, China. qinzux@163.com
authors.forename: Zu-Xing
authors.initials: ZX
authors.lastname: Qin
authors.forename: Gao-Bo
authors.initials: GB
authors.lastname: Huang
authors.forename: Jun
authors.initials: J
authors.lastname: Luo
authors.forename: Shu-Fang
authors.initials: SF
authors.lastname: Ning
authors.forename: Sheng-Sheng
authors.initials: SS
authors.lastname: Lu
authors.forename: Ke-Huan
authors.initials: KH
authors.lastname: Lu
mesh_terms: D000818:Animals; D003470:Culture Media / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D047108:Embryonic Development / Q000187:drug effects; D005260:Female; D006877:Hydroxamic Acids / Q000494:pharmacology*; D008252:Macaca fascicularis; D008297:Male; D053652:Nuclear Transfer Techniques; D013552:Swine; D013997:Time Factors; D014635:Valproic Acid / Q000494:pharmacology*
chemical_list: D003470:Culture Media; D006877:Hydroxamic Acids; C012589:trichostatin A; D014635:Valproic Acid