title: Cloning and characterization of a gene encoding novel zinc finger protein transcription factor induced under water deficit stress from rice (Oryza sativa) cv. N-22.
abstract: A gene OsZnI encoding Cys3/His1-type zinc finger protein was isolated from the water stress-induced cDNA library of rice (Oryza sativa) cv. N-22, an early maturing, deep-rooted, drought-tolerant genotype adapted to upland conditions. The in-silico analysis revealed an insert of 800 bp with an ORF of 663 nucleotides, encoding 221 amino acids. OsZnI had three distinct features--nuclear localization signal (NLS) present in Arg152-Arg168, Zn finger domain between 185-193 amino acids and 12 amino acids conserved domain in 71-82 amino acids homologous to LEA motif, and belonged to C-type family of Zn finger protein. OsZnI showed induced expression under water deficit stress.
authors.affiliation: Division of Biochemistry, Indian Agricultural Research Institute, New Delhi 110012, India.
authors.forename: Khela Ram
authors.initials: KR
authors.lastname: Soren
authors.forename: Kishwar
authors.initials: K
authors.lastname: Ali
authors.forename: Aruna
authors.initials: A
authors.lastname: Tyagi
mesh_terms: D000595:Amino Acid Sequence / Q000235:genetics; D001483:Base Sequence / Q000235:genetics; D003001:Cloning, Molecular / Q000379:methods; D017124:Conserved Sequence / Q000235:genetics; D003681:Dehydration / Q000235:genetics; D055864:Droughts; D017343:Genes, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics*; D010936:Plant Extracts / Q000235:genetics / Q000378:metabolism; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics*; D016335:Zinc Fingers / Q000235:genetics*
chemical_list: D010936:Plant Extracts; D010940:Plant Proteins; D014157:Transcription Factors