title: Mcl-PHAs produced by Pseudomonas sp. Gl01 using fed-batch cultivation with waste rapeseed oil as carbon source.
abstract: The present study describes medium-chain-length polyhydroxyalkanoates (mcl-PHAs) production by the Pseudomonas Gl01 strain isolated from mixed microbial communities utilized for PHAs synthesis. A two-step fedbatch fermentation was conducted with glucose and waste rapeseed oil as the main carbon source for obtaining cell growth and mcl-PHAs accumulation, respectively. The results show that the Pseudomonas Gl01 strain is capable of growing and accumulating mcl-PHAs using a waste oily carbon source. The biomass value reached 3.0 g/l of CDW with 20% of PHAs content within 48 h of cultivation. The polymer was purified from lyophilized cells and analyzed by gas chromatography (GC). The results revealed that the monomeric composition of the obtained polyesters depended on the available substrate. When glucose was used in the growth phase, 3-hydroxyundecanoate and 3- hydroxydodecanoate were found in the polymer composition, whereas in the PHAs-accumulating stage, the Pseudomonas Gl01 strain synthesized mcl-PHAs consisting mainly of 3- hydroxyoctanoate and 3-hydroxydecanoate. The transcriptional analysis using reverse-transcription real-time PCR reaction revealed that the phaC1 gene could be transcribed simultaneously to the phaZ gene.
authors.affiliation: Department of Environmental Biotechnology, University of Warmia and Mazury in Olsztyn, Sloneczna 45G, 10-718 Olsztyn, Poland. justyna.mozejko@uwm.edu.pl
authors.forename: Justyna
authors.initials: J
authors.lastname: Mozejko
authors.forename: Andreas
authors.initials: A
authors.lastname: Wilke
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Przybyłek
authors.forename: Slawomir
authors.initials: S
authors.lastname: Ciesielski
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D061249:Batch Cell Culture Techniques; D002244:Carbon / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism; D005229:Fatty Acids, Monounsaturated; D005285:Fermentation; D015964:Gene Expression Regulation, Bacterial; D015394:Molecular Structure; D010938:Plant Oils / Q000378:metabolism*; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis* / Q000737:chemistry; D011549:Pseudomonas / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D000074262:Rapeseed Oil; D014865:Waste Disposal, Fluid / Q000379:methods*
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D005229:Fatty Acids, Monounsaturated; D010938:Plant Oils; D054813:Polyhydroxyalkanoates; D000074262:Rapeseed Oil; D002244:Carbon