title: Purification and characterization of phytase with a wide pH adaptation from common edible mushroom Volvariella volvacea (Straw mushroom).
abstract: A novel phytase with a molecular mass of 14 kDa was isolated from fresh fruiting bodies of the common edible mushroom Volvariella volvacea (Straw mushroom). The isolation procedure involved successive chromatography on DEAE-cellulose, CM-cellulose, Affi-gel blue gel, Q-Sepharose and Superdex-75. The enzyme was a monomeric protein and was unadsorbed on DEAE-cellulose, CM-cellulose and Affi-gel blue gel, but was adsorbed on Q-Sepharose. The enzyme was purified 51.6-fold from the crude extract with 25.9% yield. Its N-terminal amino acid sequence GEDNEHDTQA exhibited low homology to the other reported phytases. The optimal pH and temperature of the purified enzyme was 5 and 45 degrees C, respectively. The enzyme was quite stable over the pH range of 3.0 to 9.0 with less than 30% change in its activity, suggesting that it can be used in a very wide pH range. The enzyme exhibited broad substrate selectivity towards various phosphorylated compounds, but lacked antifungal activity against tested plant pathogens.
authors.affiliation: State Key Laboratory for Agrobiotechnology, Department of Microbiology, China Agricultural University, Beijing 100193, China.
authors.forename: Lijing
authors.initials: L
authors.lastname: Xu
authors.forename: Guoqing
authors.initials: G
authors.lastname: Zhang
authors.forename: Hexiang
authors.initials: H
authors.lastname: Wang
authors.forename: Tzi Bun
authors.initials: TB
authors.lastname: Ng
mesh_terms: D010832:6-Phytase / Q000737:chemistry* / Q000302:isolation & purification*; D000222:Adaptation, Physiological; D002848:Chromatography, DEAE-Cellulose / Q000379:methods*; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D012685:Sepharose / Q000737:chemistry; D016415:Sequence Alignment / Q000379:methods*; D013379:Substrate Specificity; D013696:Temperature; D014227:Triazines / Q000737:chemistry; D055367:Volvariella / Q000201:enzymology*
chemical_list: D014227:Triazines; C038830:DEAE affigel blue; D012685:Sepharose; D010832:6-Phytase