title: DNA/amphiphilic block copolymer nanospheres reduce asthmatic response in a mouse model of allergic asthma.
abstract: Asthma is a chronic, inflammatory, respiratory disease caused by an abnormal reactivity against allergens. The most promising treatments for asthma are based on specific immunotherapies, but they lack efficiency and can induce deleterious side effects. Among new modalities of immunotherapy currently in development, DNA vaccination presents a promising approach, as it enables targeted immunotherapy in association with reduced allergenicity. We have developed an innovative, DNA-based vaccine against Dermatophagoides farinae 1 allergen (Der f 1), one of the allergens most commonly encountered by asthma patients in Europe. Intramuscular administration of a Der f 1-encoding plasmid formulated with the block copolymer 704 in healthy mice induced a strong humoral and cellular response with a pro-helper T cell type 1 bias. Administration of the same formulation in asthmatic mice, according to an early vaccination protocol, led to a reduction of airway hyperresponsiveness and a significant decrease in the level of inflammatory cytokines in the bronchoalveolar lavage of Der f 1-vaccinated mice.
authors.affiliation: INSERM U915, Nantes F-44000, France.
authors.forename: Fanny
authors.initials: F
authors.lastname: Beilvert
authors.forename: Adrien
authors.initials: A
authors.lastname: Tissot
authors.forename: Marie
authors.initials: M
authors.lastname: Langelot
authors.forename: Mathieu
authors.initials: M
authors.lastname: Mével
authors.forename: Benoit
authors.initials: B
authors.lastname: Chatin
authors.forename: David
authors.initials: D
authors.lastname: Lair
authors.forename: Antoine
authors.initials: A
authors.lastname: Magnan
authors.forename: Bruno
authors.initials: B
authors.lastname: Pitard
mesh_terms: D000818:Animals; D039741:Antigens, Dermatophagoides / Q000235:genetics*; D060829:Arthropod Proteins / Q000235:genetics*; D001249:Asthma / Q000276:immunology / Q000628:therapy*; D003546:Cysteine Endopeptidases / Q000235:genetics*; D004195:Disease Models, Animal; D006801:Humans; D007273:Injections, Intramuscular; D051379:Mice; D008807:Mice, Inbred BALB C; D054118:Nanospheres / Q000008:administration & dosage / Q000627:therapeutic use*; D011108:Polymers / Q000008:administration & dosage*
chemical_list: D039741:Antigens, Dermatophagoides; D060829:Arthropod Proteins; D011108:Polymers; D003546:Cysteine Endopeptidases; C451159:Dermatophagoides farinae antigen f 1