title: Separation and purification of phosvitin phosphopeptides using immobilized metal affinity nanoparticles.
abstract: Monodispersed and functional immobilized metal affinity magnetic chondroitin sodium sulfate nanoparticles (short as IMAN @ Fe (III)) were prepared and employed in extracting of Phosvitin Phosphopeptides (short as PPPs) from egg yolk. It was found that the diameter of the magnetic CS nanoparticles was about 20 nm, and they could easily be aggregated by a magnet when suspending in the aqueous solution. The adsorption equilibrium of PPPs onto the obtained nanocarriers fitted well with the Langmuir model. The adsorption capacity of PPPs onto the superparamagnetic nanoparticles was influenced by pH and the initial concentration of the peptides solution. The final nitrogen/phosphorus molar ratios (short as N/P) of PPPs from crude egg yolk peptides and phosvitin peptides were low to 5.78 and 5.23, respectively. Compared with traditional methods, the need for preparation of phosvitin before purification is obviated and the higher purity of PPPs were obtained. In conclusion, this type of IMAN @ Fe (III) would bring advantages to the conventional separation techniques of PPPs from chicken egg yolk.
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Sun
authors.forename: Yuntao
authors.initials: Y
authors.lastname: Liu
authors.forename: Junhua
authors.initials: J
authors.lastname: Li
authors.forename: Yujie
authors.initials: Y
authors.lastname: Su
authors.forename: Wenshui
authors.initials: W
authors.lastname: Xia
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Yang
mesh_terms: D000327:Adsorption; D002809:Chondroitin Sulfates / Q000737:chemistry; D002846:Chromatography, Affinity / Q000295:instrumentation* / Q000379:methods*; D004530:Egg Yolk / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D010748:Phosphopeptides / Q000302:isolation & purification*; D010774:Phosvitin / Q000302:isolation & purification*
chemical_list: D058185:Magnetite Nanoparticles; D010748:Phosphopeptides; D002809:Chondroitin Sulfates; D010774:Phosvitin