title: Multiplex real-time PCR for detection of an epidemic KPC-producing Klebsiella pneumoniae ST258 clone.
abstract: We describe a multiplex real-time PCR assay capable of identifying both the epidemic Klebsiella pneumoniae ST258 clone and bla(KPC) carbapenemase genes in a single reaction. The assay displayed excellent sensitivity (100%) and specificity (100%) for identification of ST258 clone and bla(KPC) in a collection of 75 K. pneumoniae isolates comprising 41 sequence types. Our results suggest that this assay is an effective tool for surveillance of this clone among carbapenem-resistant K. pneumoniae clinical isolates.
authors.affiliation: Public Health Research Institute Center, International Center for Public Health, University of Medicine and Dentistry of New Jersey, Newark, New Jersey, USA.
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
authors.forename: Kalyan D
authors.initials: KD
authors.lastname: Chavda
authors.forename: José R
authors.initials: JR
authors.lastname: Mediavilla
authors.forename: Yanan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Henry S
authors.initials: HS
authors.lastname: Fraimow
authors.forename: Stephen G
authors.initials: SG
authors.lastname: Jenkins
authors.forename: Michael H
authors.initials: MH
authors.lastname: Levi
authors.forename: Tao
authors.initials: T
authors.lastname: Hong
authors.forename: Albert D
authors.initials: AD
authors.lastname: Rojtman
authors.forename: Christine C
authors.initials: CC
authors.lastname: Ginocchio
authors.forename: Robert A
authors.initials: RA
authors.lastname: Bonomo
authors.forename: Barry N
authors.initials: BN
authors.lastname: Kreiswirth
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics*; D015780:Carbapenems / Q000494:pharmacology; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015780:Carbapenems; D001618:beta-Lactamases; C063912:carbapenemase