title: [Expression of neonatal Fc receptor on human nephritis and rat nephritis models].
abstract: OBJECTIVE
  To study the expression of neonatal Fc receptor in podocytes in human nephritis and immune-induced rat nephritis models: anti-Thy1.1 nephritis and Heymann nephritis.
  
  
  METHODS
  Thirty-nine cases of renal biopsies were enrolled from September 2009 to February 2010, including 8 cases of minimal change disease, 4 cases of focal segmental glomerulosclerosis, 9 cases of membranous nephropathy, 12 cases of IgA nephropathy and 6 cases of lupus nephritis. Five normal kidney tissue samples adjacent to renal clear-cell carcinoma were served as normal controls. Laser capture microdissection and real-time RT-PCR were used to assess the expression level of FcRn mRNA in glomeruli of various glomerulonephritides, and immunohistochemistry (IHC) of FcRn by SuperVision method was performed. In addition, rat models of mesangial proliferative nephritis (anti-Thy1.1 nephritis) and passive membranous nephropathy (Heymann nephritis) were established and FcRn was examined in renal tissues by IHC.
  
  
  RESULTS
  The FcRn mRNA level in lupus nephritis was statistically higher than that of normal controls (P < 0.05). FcRn protein expression by IHC was seen in lupus nephritis (6/6), membranous nephropathy (6/9) and IgA nephropathy (7/12), significantly higher than that of normal controls (0/5), P < 0.05. Minimal change disease and focal segmental glomerular sclerosis showed minimal or none expression of FcRn (1/8, 0/4 respectively) and not statistically difference from that of normal controls. Furthermore, FcRn expression in podocytes was detected in rat anti-Thy1.1 (3/5) and Heymann nephritis models (2/7) but was not detected in normal controls.
  
  
  CONCLUSIONS
  Expression of FcRn in podocytes was up-regulated in immune-induced human nephritis and rat nephritis models of anti-Thy1.1 nephritis and Heymann nephritis. FcRn may play a role in the development of immune-induced glomerulonephritis.
authors.affiliation: Department of Pathology, Shanghai Medical College, Fudan University, Shanghai 200032, China.
authors.forename: Song-tao
authors.initials: ST
authors.lastname: Feng
authors.forename: Hua-lei
authors.initials: HL
authors.lastname: Gan
authors.forename: Jian-yong
authors.initials: JY
authors.lastname: Sun
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
authors.forename: Bao-li
authors.initials: BL
authors.lastname: Liu
authors.forename: Zhong-hua
authors.initials: ZH
authors.lastname: Zhao
authors.forename: Mu-yi
authors.initials: MY
authors.lastname: Guo
authors.forename: Zhi-gang
authors.initials: ZG
authors.lastname: Zhang
mesh_terms: D000818:Animals; D005922:Glomerulonephritis, IGA / Q000378:metabolism / Q000473:pathology; D015433:Glomerulonephritis, Membranous / Q000378:metabolism* / Q000473:pathology; D005923:Glomerulosclerosis, Focal Segmental / Q000378:metabolism / Q000473:pathology; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D061346:Laser Capture Microdissection; D008181:Lupus Nephritis / Q000378:metabolism / Q000473:pathology; D008297:Male; D009393:Nephritis / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D009402:Nephrosis, Lipoid / Q000378:metabolism / Q000473:pathology; D050199:Podocytes / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D011961:Receptors, Fc / Q000235:genetics / Q000378:metabolism*; D018800:Thy-1 Antigens / Q000276:immunology / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D015395:Histocompatibility Antigens Class I; D012333:RNA, Messenger; D011961:Receptors, Fc; D018800:Thy-1 Antigens; C100688:Fc receptor, neonatal