title: Identification of EBP50 as a specific biomarker for carcinogens via the analysis of mouse lymphoma cellular proteome.
abstract: To identify specific biomarkers generated upon exposure of L5178Y mouse lymphoma cells to carcinogens, 2-DE and MALDI-TOF MS analysis were conducted using the cellular proteome of L5178Y cells that had been treated with the known carcinogens, 1,2-dibromoethane and O-nitrotoluene and the noncarcinogens, emodin and D-mannitol. Eight protein spots that showed a greater than 1.5-fold increase or decrease in intensity following carcinogen treatment compared with treatment with noncarcinogens were selected. Of the identified proteins, we focused on the candidate biomarker ERM-binding phosphoprotein 50 (EBP50), the expression of which was specifically increased in response to treatment with the carcinogens. The expression level of EBP50 was determined by western analysis using polyclonal rabbit anti-EBP50 antibody. Further, the expression level of EBP50 was increased in cells treated with seven additional carcinogens, verifying that EBP50 could serve as a specific biomarker for carcinogens.
authors.affiliation: Department of Biotechnology, Translational Research Center for Protein Function Control, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, Korea.
authors.forename: Yoen Jung
authors.initials: YJ
authors.lastname: Lee
authors.forename: In-Kwon
authors.initials: IK
authors.lastname: Choi
authors.forename: Yhun Yhong
authors.initials: YY
authors.lastname: Sheen
authors.forename: Sue Nie
authors.initials: SN
authors.lastname: Park
authors.forename: Ho Jeong
authors.initials: HJ
authors.lastname: Kwon
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D002273:Carcinogens / Q000494:pharmacology*; D045744:Cell Line, Tumor; D015946:Ethylene Dibromide / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D008223:Lymphoma; D051379:Mice; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D017923:Sodium-Hydrogen Exchangers / Q000235:genetics / Q000378:metabolism*; D014050:Toluene / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: D014408:Biomarkers, Tumor; D002273:Carcinogens; D010750:Phosphoproteins; D020543:Proteome; D017923:Sodium-Hydrogen Exchangers; C093376:sodium-hydrogen exchanger regulatory factor; D015946:Ethylene Dibromide; D014050:Toluene; C029955:2-nitrotoluene