title: A novel, universal and sensitive lateral-flow based method for the detection of multiple bacterial contamination in platelet concentrations.
abstract: In the present study, we aimed to develop a nucleic acid lateral-flow method for the rapid and sensitive detection of multiple bacteria that contaminate platelet concentrations (PCs).  Polymerase chain reaction (PCR) amplicons were produced by a set of board-range primers that recognize the conserved region of bacteria 16S rDNA, followed by hybridization with both an FITC (fluorescein isothiocyanate)-labelled probe and biotin-labelled probe, and then a nucleic acid lateral-flow dipstick (LFD) assay.  The LFD accurately identified 7 species of bacteria, but had no cross-reactivity with human genomic DNA.  The limit of detection (LOD) of the LFD assay was as low as 10(1) copies/µL of 16S rDNA for plasmid.  In the case of spiked PCs without enrichment, the detection limit of LFD for K. pneumonia was 5 CFU/mL, 6.5 × 10(4) CFU/mL for the S. epidermidis and 35 CFU/mL for P. aeruginosa.
authors.affiliation: Beijing Institute of Transfusion Medicine, P. R. China.
authors.forename: Jidong
authors.initials: J
authors.lastname: Wang
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Wang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Li
authors.forename: Shaoduo
authors.initials: S
authors.lastname: Yan
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Zhou
authors.forename: Bo
authors.initials: B
authors.lastname: Gao
authors.forename: Jianchun
authors.initials: J
authors.lastname: Peng
authors.forename: Juan
authors.initials: J
authors.lastname: DU
authors.forename: Qiuxia
authors.initials: Q
authors.lastname: Fu
authors.forename: Shuaizheng
authors.initials: S
authors.lastname: Jia
authors.forename: Juankun
authors.initials: J
authors.lastname: Zhang
authors.forename: Linsheng
authors.initials: L
authors.lastname: Zhan
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D001792:Blood Platelets / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000235:genetics / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D006801:Humans; D016133:Polymerase Chain Reaction; D013997:Time Factors
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal