title: Morphological analysis of zebrafish embryo tails.
abstract: Quantitative analysis of image-based phenotype is of significant importance in zebrafish image analysis and high-content screening. In this paper, an automatic analysis pipeline is proposed for quantification of the curvedness of zebrafish embryo tails. First, zebrafish embryos are extracted based on the active contour model, and then its skeleton line is generated by the principal graph algorithm. Several curvedness measures of tail skeleton are designed and compared, and experimental results show that joint consideration of the tail shape and its relative position relationship can result in better performance. The proposed pipeline has comparable performance to the manual analysis.
authors.affiliation: School of Computer Science and Technology, Nanjing University of Science and Technology, Nanjing, China. lujf@njust.edu.cn
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Lu
authors.forename: Tao
authors.initials: T
authors.lastname: Wu
authors.forename: Lei
authors.initials: L
authors.lastname: Cheng
authors.forename: Zhenwen
authors.initials: Z
authors.lastname: Zhu
authors.forename: Jingyu
authors.initials: J
authors.lastname: Yang
mesh_terms: D000818:Animals; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D013623:Tail / Q000196:embryology*; D015027:Zebrafish / Q000196:embryology*