title: Evaluation and prognostic significance of human tissue kallikrein-related peptidase 10 (KLK10) in colorectal cancer.
abstract: The prognosis of patients with colorectal cancer (CRC) is assessed through conventional clinicopathological parameters, which are not always accurate. Members of the human kallikrein-related peptidases gene family represent potential cancer biomarkers. The aim of this study was to investigate the expression of human tissue kallikrein-related peptidase 10 (KLK10) by immunohistochemistry in CRC, to correlate this expression with various histopathological and clinical variables, and to evaluate its significance as a predictor of disease outcome. KLK10 expression was evaluated by immunohistochemistry and a combined expression score was calculated for each case based on intensity and percentage of positivity. A statistically significant positive association was observed between KLK10 and tumor stage and liver metastases (p = 0.015 and p = 0.035, respectively). Paradoxically, a negative association was observed between KLK10 and tumor grade (p = 0.009). Kaplan-Meier survival curves and univariate analysis showed that both KLK10 expression and stage had statistically significant correlations with disease-free survival (DFS) (p = 0.030 and p < 0.001, respectively) and overall survival (p = 0.010 and p = 0.001, respectively). Cox multivariate analysis showed that both KLK10 expression and stage were independent predictors of unfavorable DFS (p = 0.057 and p = 0.001, respectively) and overall survival (p = 0.009 and p = 0.001, respectively). In conclusion, KLK10 immunostaining is an independent prognostic marker in patients with CRC.
authors.affiliation: Departments of Pathology, Metropolitan Hospital, Athens, Greece.
authors.forename: Constantina
authors.initials: C
authors.lastname: Petraki
authors.forename: Youssef M
authors.initials: YM
authors.lastname: Youssef
authors.forename: William
authors.initials: W
authors.lastname: Dubinski
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Lichner
authors.forename: Andreas
authors.initials: A
authors.lastname: Scorilas
authors.forename: Maria D
authors.initials: MD
authors.lastname: Pasic
authors.forename: Vassilios
authors.initials: V
authors.lastname: Komborozos
authors.forename: Bishoy
authors.initials: B
authors.lastname: Khalil
authors.forename: Catherine
authors.initials: C
authors.lastname: Streutker
authors.forename: Eleftherios P
authors.initials: EP
authors.lastname: Diamandis
authors.forename: George M
authors.initials: GM
authors.lastname: Yousef
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000201:enzymology* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000706:statistics & numerical data; D007610:Kallikreins / Q000378:metabolism*; D053208:Kaplan-Meier Estimate; D008113:Liver Neoplasms / Q000201:enzymology* / Q000556:secondary; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009367:Neoplasm Staging; D011237:Predictive Value of Tests; D011379:Prognosis; D016016:Proportional Hazards Models
chemical_list: D014408:Biomarkers, Tumor; C100936:KLK10 protein, human; D007610:Kallikreins