title: Electro membrane extraction of sodium diclofenac as an acidic compound from wastewater, urine, bovine milk, and plasma samples and quantification by high-performance liquid chromatography.
abstract: Electro membrane extraction (EME) as a new microextraction method was applied for extraction of sodium diclofenac (SDF) as an acidic compound from wastewater, urine, bovine milk and plasma samples. Under applied potential of 20 V during the extraction, SDF migrated from a 2.1 mL of sample solution (1mM NaOH), through a supported liquid membrane (SLM), into a 30 μL acceptor solution (10 mM NaOH), exist inside the lumen of the hollow fiber. The negative electrode was placed in the donor solution, and the positive electrode was placed in the acceptor solution. 1-octanol was immobilized in the pores of a porous hollow fiber of polypropylene as SLM. Then the extract was analyzed by means of high-performance liquid chromatography (HPLC) with UV-detection for quantification of SDF. Best results were obtained using a phosphate running electrolyte (10 mM, pH 2.5). The ranges of quantitation for different samples were 8-500 ngmL(-1). Intra- and inter-day RSDs were less than 14.5%. Under the optimized conditions, the preconcentration factors were between 31 and 66 and also the limit of detections (LODs) ranged from 2.7 ng mL(-1) to 5 ng mL(-1) in different samples. This procedure was applied to determine SDF in wastewater, bovine milk, urine and plasma samples (spiked and real samples). Extraction recoveries for different samples were between 44-95% after 5 min of extraction.
authors.affiliation: Department of Chemistry, Faculty of Sciences, Shahid Beheshti University, G. C., 1983963113, Evin, Tehran, Iran. ss-hosseiny@cc.sbu.ac.ir
authors.forename: Saied Saeed Hosseiny
authors.initials: SS
authors.lastname: Davarani
authors.forename: Ahmad
authors.initials: A
authors.lastname: Pourahadi
authors.forename: Saeed
authors.initials: S
authors.lastname: Nojavan
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Banitaba
authors.forename: Mahnaz
authors.initials: M
authors.lastname: Nasiri-Aghdam
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004008:Diclofenac / Q000032:analysis* / Q000097:blood / Q000652:urine; D055664:Electrochemical Techniques; D006863:Hydrogen-Ion Concentration; D008567:Membranes, Artificial; D008892:Milk / Q000737:chemistry*; D008962:Models, Theoretical; D010710:Phosphates / Q000737:chemistry; D012965:Sodium Chloride / Q000737:chemistry; D013696:Temperature; D014874:Water Pollutants, Chemical / Q000737:chemistry*
chemical_list: D008567:Membranes, Artificial; D010710:Phosphates; D014874:Water Pollutants, Chemical; D004008:Diclofenac; D012965:Sodium Chloride