title: Krüppel-like factor 4 inhibits the expression of interleukin-1 beta in lipopolysaccharide-induced RAW264.7 macrophages.
abstract: RAW264.7 macrophages and human peripheral blood mononuclear cells were treated with LPS to determine the expression of KLF4 and release of IL-1β. A full-length cDNA or short interference RNA of KLF4 was transfected into RAW264.7 macrophages; the expression and release of IL-1β were analyzed. The transcription and DNA binding activities of KLF4 to the IL-1β promoter were detected further. The results showed LPS treatment resulted in the increase of KLF4 level and IL-1β release; KLF4 overexpression decreased the expression of IL-1β, while KLF4 inhibition increased the expression of IL-1β; overexpression of KLF4 promoted the DNA binding activity of KLF4 to the IL-1β promoter and attenuated the transcription of IL-1β promoter, indicating an important role of KLF4 in regulating expression of IL-1β.
authors.affiliation: Laboratory of Shock, Department of Pathophysiology, School of Basic Medical Sciences, Central South University, Changsha, Hunan 410008, People's Republic of China. liujunwen@csu.edu.cn
authors.forename: Junwen
authors.initials: J
authors.lastname: Liu
authors.forename: Tao
authors.initials: T
authors.lastname: Yang
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Huali
authors.initials: H
authors.lastname: Zhang
authors.forename: Kangkai
authors.initials: K
authors.lastname: Wang
authors.forename: Meidong
authors.initials: M
authors.lastname: Liu
authors.forename: Guangwen
authors.initials: G
authors.lastname: Chen
authors.forename: Xianzhong
authors.initials: X
authors.lastname: Xiao
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D006801:Humans; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism*; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D011401:Promoter Regions, Genetic; D034622:RNA Interference
chemical_list: D053583:Interleukin-1beta; C000715467:KLF4 protein, human; C000715468:Klf4 protein, mouse; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors