title: In vitro anthelmintic activity of crude extracts of selected medicinal plants against Haemonchus contortus from sheep.
abstract: Ethanol extracts of 25 plant species were screened for anthelmintic effects against Haemonchus contortus. Ethanol extracts of each plant were used at various concentrations (10, 20 and 30%) to treat 10-day faecal cultures, incubated at 27°C with control cultures which were treated with ethanol for 48 h. Five plants with high efficacies (Ananas comosus, Aloe ferox, Allium sativum, Lespedeza cuneata and Warburgia salutaris) were selected from the first screening for further investigation using ethanol, dichloromethane and water extracts at four concentrations (2.5, 5, 10 and 20%). Ethanol was the most effective solvent. Larval counts decreased with increasing extract concentrations, of which 10 and 20% had similar effects. Lespedeza cuneata caused more than 70% mortality at all concentrations. However, there remains a need to assess in vivo efficacy of these plants.
authors.affiliation: Department of Animal Science, University of KwaZulu-Natal, Scottsville 3209, South Africa. 207529204@ukzn.ac.za
authors.forename: M
authors.initials: M
authors.lastname: Ahmed
authors.forename: M D
authors.initials: MD
authors.lastname: Laing
authors.forename: I V
authors.initials: IV
authors.lastname: Nsahlai
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000302:isolation & purification / Q000494:pharmacology*; D045424:Complex Mixtures / Q000302:isolation & purification / Q000494:pharmacology*; D006189:Haemonchus / Q000187:drug effects* / Q000302:isolation & purification / Q000502:physiology; D007814:Larva / Q000187:drug effects / Q000502:physiology; D010946:Plants, Medicinal / Q000737:chemistry*; D012756:Sheep / Q000469:parasitology*; D016019:Survival Analysis; D013696:Temperature; D013997:Time Factors
chemical_list: D000871:Anthelmintics; D045424:Complex Mixtures