title: [Validation study on a method for multiresidue analysis of pesticides in vegetables and fruits with supercritical fluid extraction].
abstract: Supercritical fluid extraction (SFE) was applied to extraction of pesticides from vegetables and fruits. Residues were extracted from homogenized samples mixed with water-absorbent polymer with supercritical carbon dioxide in a stainless steel tube, followed by elution with acetone. Co-extractives were removed by means of mini-column clean-up. Measurement was performed by GC-MS/MS. Calibration was achieved by preparing matrix-matched calibration standards to counteract matrix effects. With the Japanese method validation guideline as a reference, the method was assessed in 5 agricultural products spiked with 334 pesticides at 0.01 and 0.1 µg/g. Compounds at each level were extracted from 2 samples on 5 separate days. The trueness of the method for 189 pesticides in all samples was 70-120%, and the repeatability and within-run reproducibility were also consistent with the guideline. The trueness of the method for the other 71 pesticides was in the range of 50-70%, though the repeatability and within-run reproducibility were satisfactory. This method is available as a multiresidue analysis method for vegetables and fruits.
authors.affiliation: Nara Prefectural Institute for Hygiene and Environment, Nara, Japan. k.uranishi@ihe.pref.nara.jp
authors.forename: Katsushige
authors.initials: K
authors.lastname: Uranishi
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Yamashita
authors.forename: Keigo
authors.initials: K
authors.lastname: Yamamoto
mesh_terms: D002849:Chromatography, Gas; D005638:Fruit / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D010573:Pesticide Residues / Q000032:analysis*; D015203:Reproducibility of Results; D014675:Vegetables / Q000737:chemistry*
chemical_list: D010573:Pesticide Residues