title: Genetic and cytogenetic analysis of the American cherry fruit fly, Rhagoletis cingulata (Diptera: Tephritidae).
abstract: The American eastern cherry fruit fly, Rhagoletis cingulata, a pest of cherries in the western hemisphere, invaded Europe in 1983, and since then dispersed to several European countries. Information on the genetics and cytogenetics of this pest is very scarce. The mitotic karyotype and detailed photographic maps of the salivary gland polytene chromosomes of R. cingulata are presented here. The mitotic metaphase complement consists of six pairs of chromosomes with the sex chromosomes being very small and similar in size. The analysis of the salivary gland polytene complement shows a total number of five long chromosomes (10 polytene arms), which correspond to the five autosomes of the mitotic nuclei and an extrachromosomal heterochromatic mass, which corresponds to the sex chromosomes. The banding patterns and the most characteristic features and prominent landmarks of each polytene chromosome are presented and discussed. Chromosomal homologies between R. cingulata, R. completa and R. cerasi are also proposed, based on the comparison of chromosome banding patterns. Furthermore, the detection and characterization of Wolbachia pipientis in the R. cingulata population studied is presented and the potential correlation with the asynaptic phenomena found in its polytene complement is discussed. In addition, 10 out of 24 microsatellite markers developed for other Rhagoletis species are cross-amplified, evaluated and proposed as useful markers for population and genetic studies in R. cingulata.
authors.affiliation: Department of Genetics, Development and Molecular Biology, Faculty of Sciences, School of Biology, Aristotle University of Thessaloniki, Thessaloníki, Greece.
authors.forename: Elena
authors.initials: E
authors.lastname: Drosopoulou
authors.forename: Antonios A
authors.initials: AA
authors.lastname: Augustinos
authors.forename: Ifigeneia
authors.initials: I
authors.lastname: Nakou
authors.forename: Kirsten
authors.initials: K
authors.lastname: Koeppler
authors.forename: Ilias
authors.initials: I
authors.lastname: Kounatidis
authors.forename: Heidrun
authors.initials: H
authors.lastname: Vogt
authors.forename: Nikolaos T
authors.initials: NT
authors.lastname: Papadopoulos
authors.forename: Kostas
authors.initials: K
authors.lastname: Bourtzis
authors.forename: Penelope
authors.initials: P
authors.lastname: Mavragani-Tsipidou
mesh_terms: D000483:Alleles; D000818:Animals; D059006:Chromosomes, Insect; D020732:Cytogenetic Analysis; D005260:Female; D017344:Genes, Insect; D059785:Karyotype; D008297:Male; D018895:Microsatellite Repeats; D008938:Mitosis; D010802:Phylogeny; D059007:Polytene Chromosomes; D012730:Sex Chromosomes; D033621:Tephritidae / Q000235:genetics*