title: High-throughput production and structural characterization of libraries of self-assembly lipidic cubic phase materials.
abstract: A protocol is presented for the high-throughput (HT) production of lyotropic liquid crystalline phases from libraries of lipids and lipid mixtures using standard liquid dispensing robotics, implementing methods that circumvent the problems traditionally associated with handling the highly viscous cubic phase. In addition, the ability to structurally characterize lipidic phases and assess functionality for membrane proteins contained within cubic phases, in a HT manner, is demonstrated. The techniques are combined and exemplified using the application of membrane protein crystallization within lipidic cubic phases.
authors.affiliation: CSIRO Materials Science and Engineering, Parkville, Victoria, Australia. connie.darmanin@csiro.au
authors.forename: Connie
authors.initials: C
authors.lastname: Darmanin
authors.forename: Charlotte E
authors.initials: CE
authors.lastname: Conn
authors.forename: Janet
authors.initials: J
authors.lastname: Newman
authors.forename: Xavier
authors.initials: X
authors.lastname: Mulet
authors.forename: Shane A
authors.initials: SA
authors.lastname: Seabrook
authors.forename: Yi-Lynn
authors.initials: YL
authors.lastname: Liang
authors.forename: Adrian
authors.initials: A
authors.lastname: Hawley
authors.forename: Nigel
authors.initials: N
authors.lastname: Kirby
authors.forename: Joseph N
authors.initials: JN
authors.lastname: Varghese
authors.forename: Calum J
authors.initials: CJ
authors.lastname: Drummond
mesh_terms: D001436:Bacteriorhodopsins / Q000737:chemistry*; D003460:Crystallization; D057166:High-Throughput Screening Assays; D006801:Humans; D008055:Lipids / Q000138:chemical synthesis* / Q000737:chemistry*; D050866:Liquid Crystals / Q000737:chemistry; D008958:Models, Molecular; D019151:Peptide Library; D011487:Protein Conformation; D017448:Receptors, Dopamine D2 / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D011969:Receptors, Histamine H1 / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D015203:Reproducibility of Results; D012371:Robotics; D053838:Scattering, Small Angle; D014961:X-Ray Diffraction
chemical_list: D008055:Lipids; D019151:Peptide Library; D017448:Receptors, Dopamine D2; D011969:Receptors, Histamine H1; D001436:Bacteriorhodopsins