title: [Protective effects of Z-ligustilide against cytotoxicity induced by Abeta25-35 in neuron cells].
abstract: OBJECTIVE
  To investigate the effects of Z-ligustilide (LIG) on Abeta25-35-induced cytotoxicity in human neuroblastoma SH-SY5Y cells.
  
  
  METHODS
  Cells were incubated with 0.1, 1.0, 2.5, 5.0 microg/mL LIG and then were exposed to 50 micromol/L Abeta25-35 to induce cytotoxicity in SH-SY5Y cell to establish the AD model in vitro. Cell viability was test by MTT method. Pro- and anti-apoptosis protein levels were investigated by Western blot.
  
  
  RESULTS
  After exposure to Abeta25-35, the cell viability decreased significantly, and the expression of pro-apoptosis protein--Bax, cleaved caspase 3, cytochrome C, caspase 8 was up regulated while the anti-apoptosis protein Bcl-2 was down regulated. However, the treatment of LIG (0.1, 1.0, 2.5, 5.0 microg/mL) significantly restored these changes.
  
  
  CONCLUSIONS
  LIG has the protective effect against cytotoxicity induced by in SH-SY5Y cells via inhibit the apoptosis induced by Abeta25-35.
authors.affiliation: Department of Pharmacology and Biopharmaceutics, West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
authors.forename: Meng-Xue
authors.initials: MX
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Yu
authors.forename: Jun-Rong
authors.initials: JR
authors.lastname: Du
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000494:pharmacology; D000544:Alzheimer Disease / Q000473:pathology; D016229:Amyloid beta-Peptides / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D006801:Humans; D009447:Neuroblastoma / Q000473:pathology; D009474:Neurons / Q000473:pathology*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010446:Peptide Fragments / Q000037:antagonists & inhibitors* / Q000633:toxicity*
chemical_list: D016229:Amyloid beta-Peptides; D018696:Neuroprotective Agents; D010446:Peptide Fragments; C074636:amyloid beta-protein (25-35); C027820:ligustilide; D015107:4-Butyrolactone