title: Green biosynthesis of floxuridine by immobilized microorganisms.
abstract: This work describes an efficient, simple, and green bioprocess for obtaining 5-halogenated pyrimidine nucleosides from thymidine by transglycosylation using whole cells. Biosynthesis of 5-fluoro-2'-deoxyuridine (floxuridine) was achieved by free and immobilized Aeromonas salmonicida ATCC 27013 with an 80% and 65% conversion occurring in 1 h, respectively. The immobilized biocatalyst was stable for more than 4 months in storage conditions (4 °C) and could be reused at least 30 times without loss of its activity. This microorganism was able to biosynthesize 2.0 mg L(-1) min(-1) (60%) of 5-chloro-2'-deoxyuridine in 3 h. These halogenated pyrimidine 2'-deoxynucleosides are used as antitumoral agents.
authors.affiliation: Laboratorio de Investigaciones en Biotecnología Sustentable (LIBioS), Universidad Nacional de Quilmes, Bernal, Argentina.
authors.forename: Cintia W
authors.initials: CW
authors.lastname: Rivero
authors.forename: Claudia N
authors.initials: CN
authors.lastname: Britos
authors.forename: Mario E
authors.initials: ME
authors.lastname: Lozano
authors.forename: Jose V
authors.initials: JV
authors.lastname: Sinisterra
authors.forename: Jorge A
authors.initials: JA
authors.lastname: Trelles
mesh_terms: D048409:Aeromonas salmonicida / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods; D001711:Biotransformation; D018914:Cells, Immobilized / Q000378:metabolism; D005467:Floxuridine / Q000378:metabolism*; D006031:Glycosylation; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors
chemical_list: D005467:Floxuridine; D013936:Thymidine