title: Characterization of hepatocellular carcinoma cell lines based on cell             adhesion molecules.
abstract: Many studies which focus on the molecules and mechanisms related to the             characteristics of the cancer have been performed. In particular, cell adhesion             molecules (CAMs) are known to play a central role in the adhesion of cancer cells             to vascular endothelial cells. In this study, the expression of CAMs in hepatocellular             carcinoma (HCC) cell lines was analyzed and correlated with the characteristics             of various HCC cell lines. Eight human HCC cell lines were used in this study.             We analyzed the expression of ICAM-1, E-selectin and the integrin subunits of             HCC cell lines by western blot analysis and ELISA kit. We estimated the expression             of integrin-α5 using western blot analysis and RT-PCR to compare the expression             at the gene level with the protein level. In addition, we determined the expression             of TGF-β1, as one of the markers for the cellular activity compared to the levels             of expression with the expression of integrin-α3 and -α5. ICAM-1 was highly expressed             in all of the cell lines except SNU398 and Hep3B, which exhibit a more aggressive             nature among the studied HCC cell lines. E-selectin and integrin subunits varied             in all HCC cell lines. In particular, integrin-β2 was highly expressed on all             HCC cell lines. In conclusion, the levels of expression of the CAMs may not affect             cellular activity, morphology or tumorigenicity. However, most HCC cell lines             show various expressions of CAMs, suggesting that HCC cell lines expressing the             major CAMs remain candidates for molecular targeted therapy, which may need to             be patient-tailored for therapy according to the molecular profile.
authors.affiliation: Department of Surgery, Korea University Guro Hospital, Seoul,                     Republic of Korea.
authors.forename: Cheol Woong
authors.initials: CW
authors.lastname: Jung
authors.forename: Tae-Jin
authors.initials: TJ
authors.lastname: Song
authors.forename: Kun-Ok
authors.initials: KO
authors.lastname: Lee
authors.forename: Sae Byeol
authors.initials: SB
authors.lastname: Choi
authors.forename: Wan Bae
authors.initials: WB
authors.lastname: Kim
authors.forename: Sung Ock
authors.initials: SO
authors.lastname: Suh
authors.forename: Young Chul
authors.initials: YC
authors.lastname: Kim
authors.forename: Sang Yong
authors.initials: SY
authors.lastname: Choi
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D045744:Cell Line, Tumor; D019040:E-Selectin / Q000378:metabolism; D006801:Humans; D016023:Integrins / Q000235:genetics / Q000378:metabolism; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism*; D008297:Male; D021122:Protein Subunits / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
chemical_list: D015815:Cell Adhesion Molecules; D019040:E-Selectin; D016023:Integrins; D021122:Protein Subunits; D053773:Transforming Growth Factor beta1; D018799:Intercellular Adhesion Molecule-1