title: Gordonia alkaliphila sp. nov., an actinomycete isolated from tidal flat sediment.
abstract: A Gram-stain-positive, non-motile, aerobic actinobacterium, designated strain CJ10(T), was isolated from tidal flat sediment from the Yellow Sea in South Korea. Strain CJ10(T) grew on tryptic soy agar in the presence of 0-4 % (w/v) NaCl (optimum growth in the absence of NaCl) and at pH 6-11 (optimum pH 9). On the basis of 16S rRNA gene sequence analysis, strain CJ10(T) belonged to the genus Gordonia and showed the highest sequence similarity to Gordonia hirsuta DSM 44140(T) (97.9 %) and Gordonia hydrophobica DSM 44015(T) (97.6 %). DNA-DNA relatedness levels of strain CJ10(T) were 47.4 % (CJ10(T) as probe) and 42.2 % (G. hirsuta DSM 44140(T) as probe) to G. hirsuta DSM 44140(T) and 8.6 % (CJ10(T) as probe) and 9.3 % (G. hydrophobica DSM 44015(T) as probe) to G. hydrophobica DSM 44015(T). The major isoprenoid quinone was MK-9(H(2)). The polar lipid profile of strain CJ10(T) consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The predominant cellular fatty acids were C(18 : 1)ω9c (38.0 %), C(16 : 0) (30.1 %) and summed feature 3 (C(16 : 1)ω6c and/or C(16 : 1)ω7c; 17.4 %). The DNA G+C content was 67.7 mol%. Therefore, the results from our polyphasic taxonomic study suggest that strain CJ10(T) represents a novel species in the genus Gordonia, for which the name Gordonia alkaliphila sp. nov. is proposed; the type strain is CJ10(T) (= KACC 16561(T) = JCM 18077(T)).
authors.affiliation: Department of Biotechnology (BK21 Program), Chung-Ang University, Anseong 456-756, Republic of Korea.
authors.forename: Ju-Hee
authors.initials: JH
authors.lastname: Cha
authors.affiliation: Department of Biotechnology (BK21 Program), Chung-Ang University, Anseong 456-756, Republic of Korea.
authors.forename: Chang-Jun
authors.initials: CJ
authors.lastname: Cha
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D033601:Gordonia Bacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9