title: Fibrinoid reaction after lens extraction in rabbit eyes.
abstract: OBJECTIVE
  To measure the inflammatory reaction in the anterior chamber after lens extraction in a rabbit model and to evaluate the effect of nonsteroidal antiinflammatory drugs (NSAIDs) or steroids on the amount of inflammation as measured by fibrinogen levels in the aqueous humor.
  
  
  METHODS
  Animal laboratory, Goldschleger Research Institute, Tel Aviv University, Sheba Medical Center, Ramat Gan, Israel.
  
  
  METHODS
  Experimental study.
  
  
  METHODS
  Twenty-six eyes of New Zealand white rabbits had lens extraction surgery. One day later, aqueous humor (∼0.1 mL) was withdrawn from the anterior chamber and examined for fibrinogen concentration. Control rabbits received no treatment (9 eyes) or artificial tear eyedrops (5 eyes). One study group received NSAID drops (diclofenac) (6 eyes), and another study group received steroid drops (dexamethasone-neomycin) (6 eyes). All rabbits were treated hourly for 9 applications. Aqueous humor (∼0.1 mL) was withdrawn from the anterior chamber and examined for fibrinogen concentration 1 day later. Fibrinogen levels were also measured in the aqueous in 8 unoperated eyes.
  
  
  RESULTS
  Steroid-treated eyes achieved the lowest inflammatory score, followed by NSAID eyes, artificial tears eyes, and untreated eyes. The mean fibrinogen concentrations in the aqueous humor were 69.1 mg% untreated, 52.0 mg% artificial tears, 18.5 mg% NSAIDs, and 2.8 mg% steroids (P=.002).
  
  
  CONCLUSIONS
  Measurement of aqueous fibrinogen after lens extraction surgery in a rabbit animal model was simple and provided a useful parameter for precise evaluation of postoperative intraocular reaction. Steroids and NSAIDs were effective in reducing postoperative inflammation. Steroids reduced inflammation to almost undetectable values.
  
  
  BACKGROUND
  No author has a financial or proprietary interest in any material or method mentioned.
authors.affiliation: Goldschleger Eye Institute, Sheba Medical Center, Tel-Hashomer, Israel. guybensimon@gmail.com
authors.forename: Guy J
authors.initials: GJ
authors.lastname: Ben Simon
authors.forename: Gili
authors.initials: G
authors.lastname: Kenet
authors.forename: Abraham
authors.initials: A
authors.lastname: Spierer
mesh_terms: D000210:Acute-Phase Reaction / Q000188:drug therapy / Q000209:etiology* / Q000378:metabolism; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D001082:Aqueous Humor / Q000378:metabolism*; D002387:Cataract Extraction; D003907:Dexamethasone / Q000494:pharmacology; D004008:Diclofenac / Q000494:pharmacology; D005337:Fibrin / Q000378:metabolism; D005340:Fibrinogen / Q000378:metabolism*; D005938:Glucocorticoids / Q000494:pharmacology; D007249:Inflammation / Q000188:drug therapy / Q000209:etiology / Q000378:metabolism; D008297:Male; D011183:Postoperative Complications; D011817:Rabbits
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D005938:Glucocorticoids; D004008:Diclofenac; D003907:Dexamethasone; D005337:Fibrin; D005340:Fibrinogen