title: [Comparative analyses of allergens between landlocked and anadromous species of masu salmon Oncorhynchus masou masou].
abstract: Although the difference in allergenicity between landlocked and anadromous salmon is little understood, only anadromous salmon are recommended to be labeled in the current allergen labeling system. This study was designed to examine the allergenic potency of landlocked species (yamame) and anadromous species (sakuramasu) of masu salmon Oncorhynchus masou masou, with special reference to parvalbumin, a known major fish allergen. Analysis of the heated extracts by SDS-PAGE suggested that yamame contains parvalbumin in the muscle at considerably higher levels, as compared with sakuramasu. In accordance with this, the parvalbumin content in the muscle of yamame (1.8-7.8 mg/g), determined by visible-light ELISA, was significantly higher than that of sakuramasu (0.28-0.52 mg/g). Furthermore, fluorescence ELISA experiments showed that the heated extract from yamame reacts with serum from fish-allergic patients more strongly than that from sakuramasu. Three parvalbumin isoforms (PA-I, -II and -III) were individually purified from yamame and sakuramasu by gel filtration and reverse-phase HPLC. Based on the retention times in reverse-phase HPLC and the molecular weights estimated by MALDI/TOF-MS, PA-I, -II and -III from yamame were judged to be identical with PA-I, -II and -III from sakuramasu, respectively. Taken together, our data indicate that landlocked masu salmon (yamame) is more allergenic than anadromous salmon (sakuramasu).
authors.affiliation: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Tokyo, Japan.
authors.forename: Kuniyoshi
authors.initials: K
authors.lastname: Shimakura
authors.forename: Yukina
authors.initials: Y
authors.lastname: Wataya
authors.forename: Kazuo
authors.initials: K
authors.lastname: Shiomi
mesh_terms: D000485:Allergens / Q000032:analysis*; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D017683:Oncorhynchus / Q000276:immunology*; D010320:Parvalbumins / Q000032:analysis
chemical_list: D000485:Allergens; D010320:Parvalbumins