title: Variations in xanthophyll composition in etiolated seedlings of Arabidopsis thaliana correlate with protochlorophyllide accumulation.
abstract: Protochlorophyllide (Pchlide) accumulation and xantophyll composition were studied in 5-day old etiolated seedlings of three ecotypes of Arabidopsis thaliana: Columbia (Col-0), Landsberg erecta (Ler) and Wassiliewska (Ws). The total Pchlide level as measured by fluorescence spectroscopy varied significantly between ecotypes. A rapid HPLC method revealed quantitative differences in carotenoid composition. It was found that in the Ler ecotype any enhanced accumulation of Pchlide correlates with an increased level of lutein, suggesting the role of enzymes involved in lutein synthesis in cross-regulation between chlorophyll and carotenoid biosynthetic pathways. The function of the dark-accumulated carotenoid pool in seedling de-etiolation is discussed.
authors.affiliation: Department of Plant Physiology and Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Myśliwa-Kurdziel
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Jemioła-Rzemińska
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Turek
authors.forename: Kazimierz
authors.initials: K
authors.lastname: Strzałka
authors.forename: Przemysław
authors.initials: P
authors.lastname: Malec
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D011521:Protochlorophyllide / Q000378:metabolism*; D036226:Seedlings / Q000378:metabolism*; D024341:Xanthophylls / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D024341:Xanthophylls; D011521:Protochlorophyllide