title: Prospecting for ice association: characterization of freeze-thaw selected enrichment cultures from latitudinally distant soils.
abstract: Freeze-thaw stress has previously been shown to alter soil community structure and function. We sought to further investigate this stress on enriched microbial consortia with the aim of identifying microbes with ice-associating adaptations that facilitate survival. Enrichments were established to obtain culturable psychrotolerant microbes from soil samples from the latitudinal extremes of the Canadian Shield plateau. The resulting consortia were subjected to consecutive freeze-thaw cycles, and survivors were putatively identified by their 16S rRNA gene sequences. Even though the northerly site was exposed to longer, colder winters and large spring-time temperature fluctuations, the selective regime similarly affected both enriched consortia. Quantitative PCR and metagenomic sequencing were used to determine the frequency of a subset of the resistant microbes in the original enrichments. The metagenomes showed 22 initial genera, only 6 survived and these were not dominant prior to selection. When survivors were assayed for ice recrystallization inhibition and ice nucleation activities, over 60% had at least one of these properties. These phenotypes were not more prevalent in the northern enrichment, indicating that regarding these adaptations, the enrichment strategy yielded seemingly functionally similar consortia from each site.
authors.affiliation: Department of Biology, Queen's University, Kingston, ON K7L 3N6, Canada.
authors.forename: Sandra L
authors.initials: SL
authors.lastname: Wilson
authors.forename: Paul
authors.initials: P
authors.lastname: Grogan
authors.forename: Virginia K
authors.initials: VK
authors.lastname: Walker
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D002170:Canada; D003080:Cold Temperature; D005615:Freezing; D020459:Genes, rRNA; D007053:Ice; D054892:Metagenome / Q000502:physiology*; D059013:Microbial Consortia; D016133:Polymerase Chain Reaction; D012621:Seasons; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology
chemical_list: D007053:Ice; D012987:Soil