title: Development of a  species-specific sequence-characterized amplified region marker for roses.
abstract: DNA  fingerprints of four rose species, Rosa centifolia, R.  Gruss-an-Teplitz, R. bourboniana, and R. damascena, were  developed using RAPD-PCR. We identified a unique polymorphic band in R.  centifolia. This 762-bp fragment was produced by the random primer GLI-2.  The fragment was eluted and directly cloned in a TA cloning vector, pTZ57R/T.  Digestion of the plasmid with EcoRI confirmed the cloning of GLI-2(762) in  pTZ57R/T. A second enzyme, PstI, used in combination with EcoRI,  gave complete digestion of the plasmid, and the 762-bp fragment was confirmed  on the gel. Subsequently, the polymorphic amplicon was sequenced with an AB1  373 DNA sequencer system using the PRISM(TM) Ready  Reaction DyeDeoxy(TM) Terminator Cycle Sequencing kit.  After sequencing, specific primers (23 bp long) were designed based on the  sequence of the flanking regions of the original RAPD fragment. These primers  will effectively allow fingerprinting for the identification of R.  centifolia species. In essence, we developed an SCAR marker to authenticate  the identity of R. centifolia species and to distinguish it from its  substitutes. Such techniques are required not only to complement conventional  parameters in creating the passport data of commercial and medicinal products  of rose, but also for routine quality control in commercial and government  rosaries and rose nurseries.
authors.affiliation: Centre of Agricultural Biochemistry and Biotechnology, University of Agriculture, Faisalabad, Pakistan.
authors.forename: S
authors.initials: S
authors.lastname: Riaz
authors.forename: B
authors.initials: B
authors.lastname: Sadia
authors.forename: F S
authors.initials: FS
authors.lastname: Awan
authors.forename: I A
authors.initials: IA
authors.lastname: Khan
authors.forename: H A
authors.initials: HA
authors.lastname: Sadaqat
authors.forename: I A
authors.initials: IA
authors.lastname: Khan
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D016172:DNA Fingerprinting; D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics*; D014644:Genetic Variation; D008969:Molecular Sequence Data; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods; D027826:Rosa / Q000145:classification* / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods*; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers