title: A simple and effective strategy for labeling cysteine sulfenic acid in proteins by utilization of β-ketoesters as cleavable probes.
abstract: β-ketoesters are robust probes for labeling sulfenic acid (-SOH) proteins allowing quantitative cleavage of the tag for improved analysis of the labeled peptides by MS.
authors.affiliation: Department of Internal Medicine, Section on Molecular Medicine, Wake Forest School of Medicine, Medical Center Blvd., Winston-Salem, NC 27157, USA.
authors.forename: Jiang
authors.initials: J
authors.lastname: Qian
authors.forename: Revati
authors.initials: R
authors.lastname: Wani
authors.forename: Chananat
authors.initials: C
authors.lastname: Klomsiri
authors.forename: Leslie B
authors.initials: LB
authors.lastname: Poole
authors.forename: Allen W
authors.initials: AW
authors.lastname: Tsang
authors.forename: Cristina M
authors.initials: CM
authors.lastname: Furdui
mesh_terms: D000818:Animals; D002470:Cell Survival; D003545:Cysteine / Q000031:analogs & derivatives* / Q000032:analysis; D004952:Esters / Q000737:chemistry; D007659:Ketones / Q000737:chemistry; D051379:Mice; D008958:Models, Molecular; D041681:NIH 3T3 Cells; D011506:Proteins / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D013194:Staining and Labeling; D013434:Sulfenic Acids / Q000032:analysis*
chemical_list: D004952:Esters; D007659:Ketones; D011506:Proteins; D013434:Sulfenic Acids; C100870:cysteinesulfenic acid; D003545:Cysteine