title: Modeling neural differentiation on micropatterned substrates coated with neural matrix components.
abstract: Topographical and biochemical characteristics of the substrate are critical for neuronal differentiation including axonal outgrowth and regeneration of neural circuits in vivo. Contact stimuli and signaling molecules allow neurons to develop and stabilize synaptic contacts. Here we present the development, characterization and functional validation of a new polymeric support able to induce neuronal differentiation in both PC12 cell line and adult primary skin-derived precursor cells (SKPs) in vitro. By combining a photolithographic technique with use of neural extracellular matrix (ECM) as a substrate, a biocompatible and efficient microenvironment for neuronal differentiation was developed.
authors.affiliation: Biomaterials-Tissue Engineering Unit, Tecnalia Research and Innovation San Sebastian, Spain.
authors.forename: Patricia
authors.initials: P
authors.lastname: García-Parra
authors.forename: Fabio
authors.initials: F
authors.lastname: Cavaliere
authors.forename: Marcos
authors.initials: M
authors.lastname: Maroto
authors.forename: Leire
authors.initials: L
authors.lastname: Bilbao
authors.forename: Isabel
authors.initials: I
authors.lastname: Obieta
authors.forename: Adolfo
authors.initials: A
authors.lastname: López de Munain
authors.forename: José Iñaki
authors.initials: JI
authors.lastname: Alava
authors.forename: Ander
authors.initials: A
authors.lastname: Izeta