title: Aluminum stress response in rice: effects on membrane lipid composition and expression of lipid biosynthesis genes.
abstract: The presence of aluminum (Al) in acidic soils is a major abiotic stress limiting the production of cultivated plants. Cell membranes are the main targets of environmental stresses and there is growing evidence for the involvement of membrane lipids in plant adaptation. The aim of this study was to evaluate the mid-long effects of Al on membrane lipid content and composition in the roots and shoots of rice plants grown under hydroponic conditions. Four rice cultivars were compared: two acknowledged as Al-resistant (Koshihikari) and Al-sensitive (Kasalath), respectively, and two Vietnamese cultivars, OM6073 and OM1490. Al treatment inhibited root and shoot growth in the sensitive cultivars and the observed changes in root and shoot lipid and fatty acid composition revealed patterns associated with Al sensitivity: larger decreases in lipid content and decreases in fatty acid unsaturation. In the roots, phospholipids, and particularly phosphatidylcholine (PC), decreased dramatically in the susceptible cultivars whereas the amount of lipid classes remained unchanged in the tolerant ones. In the shoots, the glycolipids monogalactosyldiacylglycerol and digalactosyldiacylglycerol as well as PC were mostly affected by Al treatment in the susceptible varieties. mRNA accumulation corresponding to genes coding for galactolipid synthases, enzymes of the PC and phosphatidylethanolamine biosynthetic pathways and fatty acid desaturases correlated well with changes in lipid contents in roots and partly explained lipid changes in leaves. The results suggested that the capacity to maintain the proper functioning of some lipid biosynthetic activities and hence the stability of lipid composition may help the rice plant to withstand Al stress.
authors.affiliation: Université Paris Est-Créteil, Ecophysiologie Moléculaire, UMR Bioemco 7618, Equipe IBIOS, 61 avenue du Général de Gaulle, 94010 Créteil Cedex, France.
authors.forename: Van-Biet
authors.initials: VB
authors.lastname: Huynh
authors.forename: Anne
authors.initials: A
authors.lastname: Repellin
authors.forename: Yasmine
authors.initials: Y
authors.lastname: Zuily-Fodil
authors.forename: Anh-Thu
authors.initials: AT
authors.lastname: Pham-Thi
mesh_terms: D000535:Aluminum / Q000032:analysis / Q000494:pharmacology*; D018533:Biomass; D053898:Biosynthetic Pathways; D044943:Fatty Acid Desaturases / Q000235:genetics; D038983:Galactolipids / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics*; D018527:Hydroponics; D008563:Membrane Lipids / Q000235:genetics / Q000378:metabolism*; D012275:Oryza / Q000187:drug effects / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D010713:Phosphatidylcholines / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics*; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D018520:Plant Shoots / Q000187:drug effects / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics; D013312:Stress, Physiological; D013997:Time Factors
chemical_list: D038983:Galactolipids; D008563:Membrane Lipids; D010713:Phosphatidylcholines; D010743:Phospholipids; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; C007388:digalactosyldiacylglycerol; C009909:monogalactosyldiacylglycerol; D000535:Aluminum; D044943:Fatty Acid Desaturases