title: In vitro leishmanicidal activity of N-dodecyl-1,2-ethanediamine.
abstract: Polyamine biosynthesis and inhibition in parasites have been an attractive chemotherapeutic approach in the design of novel antiparasitic drugs. We study in this work the effect of N-dodecyl-1,2-ethylenediamine (NDDE) on the morphology and replication of Leishmania using macrophages cultured from the peritoneal exudate of mice infected in vitro with three species of Leishmania: Leishmania (Leishmania) amazonensis, Leishmania (Viannia) brasiliensis and Leishmania (Leishmania) chagasi. The results showed that NDDE inhibited Leishmania amastigotes multiplication into inflammatory peritoneal cells in concentrations which were not toxic to mammalian cells (0.5-1μg/mL). An intracellular disorganization of the promastigote forms was observed by transmission electron microscopy after 3 to 24h of treatment with 1μg/mL NDDE, suggesting that this compound affects the viability of the parasite by an autophagy pathway.
authors.affiliation: Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Pará 1720, Uberlândia, MG, Brazil.
authors.forename: Alexandre L Neves
authors.initials: AL
authors.lastname: Silva
authors.forename: Camila M
authors.initials: CM
authors.lastname: Adade
authors.forename: Fernanda M
authors.initials: FM
authors.lastname: Shoyama
authors.forename: Cecílio Purcino S
authors.initials: CP
authors.lastname: Neto
authors.forename: Thaís S
authors.initials: TS
authors.lastname: Padrón
authors.forename: Mauro V
authors.initials: MV
authors.lastname: de Almeida
authors.forename: Carlos A de Magalhães
authors.initials: CA
authors.lastname: Rezende
authors.forename: Claudio V
authors.initials: CV
authors.lastname: Silva
authors.forename: Maria A
authors.initials: MA
authors.lastname: Souza
mesh_terms: D000818:Animals; D000981:Antiprotozoal Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D004261:DNA Replication / Q000187:drug effects*; D005029:Ethylenediamines / Q000494:pharmacology*; D006801:Humans; D007891:Leishmania / Q000187:drug effects* / Q000648:ultrastructure; D017737:Macrophages, Peritoneal / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D010271:Parasites / Q000187:drug effects
chemical_list: D000981:Antiprotozoal Agents; D005029:Ethylenediamines; C575906:N-dodecyl-1,2-ethanediamine; C031234:ethylenediamine