title: A label-free fluorescence DNA probe based on ligation reaction with quadruplex formation for highly sensitive and selective detection of nicotinamide adenine dinucleotide.
abstract: A simple label-free fluorescent sensing scheme for sensitive and selective detection of nicotinamide adenine dinucleotide (NAD(+)) has been developed based on DNA ligation reaction with ligand-responsive quadruplex formation. This approach can detect 0.5 nM NAD(+) with high selectivity against other NAD(+) analogs.
authors.affiliation: State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
authors.forename: Jingjin
authors.initials: J
authors.lastname: Zhao
authors.forename: Liangliang
authors.initials: L
authors.lastname: Zhang
authors.forename: Jianhui
authors.initials: J
authors.lastname: Jiang
authors.forename: Guoli
authors.initials: G
authors.lastname: Shen
authors.forename: Ruqin
authors.initials: R
authors.lastname: Yu
mesh_terms: D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D005456:Fluorescent Dyes / Q000737:chemistry*; D054856:G-Quadruplexes; D009243:NAD / Q000032:analysis* / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D009243:NAD; D004247:DNA