title: Ramlibacter ginsenosidimutans sp. nov., with ginsenoside-converting activity.
abstract: A novel beta-proteobacterium, designated BXN5-27(T), was isolated from soil of a ginseng field of Baekdu Mountain in China, and was characterized using a polyphasic approach. The strain was Gram-staining-negative, aerobic, motile, non-spore-forming, and rod shaped. Strain BXN5-27(T) exhibited beta-glucosidase activity that was responsible for its ability to transform ginsenoside Rb₁ (one of the dominant active components of ginseng) to compound Rd. Phylogenetic analysis based on 16S rRNA gene sequences showed that this strain belonged to the family Comamonadaceae; it was most closely related to Ramlibacter henchirensis TMB834(T) and Ramlibacter tataouinensis TTB310(T) (96.4% and 96.3% similarity, respectively). The G+C content of the genomic DNA was 68.1%. The major menaquinone was Q-8. The major fatty acids were C₁₆:₀, summed feature 4 (comprising C₁₆:₁ omega7c and/or iso-C₁₅:₀ 2OH), and C₁₇:₀ cyclo. Genomic and chemotaxonomic data supported the affiliation of strain BXN5-27(T) to the genus Ramlibacter. However, physiological and biochemical tests differentiated it phenotypically from the other established species of Ramlibacter. Therefore, the isolate represents a novel species, for which the name Ramlibacter ginsenosidimutans sp. nov. is proposed, with the type strain being BXN5-27(T) (= DSM 23480(T) = LMG 24525(T) = KCTC 22276(T)).
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea.
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.forename: Dong-Shan
authors.initials: DS
authors.lastname: An
authors.forename: Song-Gun
authors.initials: SG
authors.lastname: Kim
authors.forename: Feng-Xie
authors.initials: FX
authors.lastname: Jin
authors.forename: Sun-Chang
authors.initials: SC
authors.lastname: Kim
authors.forename: Sung-Taik
authors.initials: ST
authors.lastname: Lee
authors.forename: Wan-Taek
authors.initials: WT
authors.lastname: Im
mesh_terms: D001711:Biotransformation; D002681:China; D042621:Comamonadaceae / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D036145:Ginsenosides / Q000378:metabolism*; D008969:Molecular Sequence Data; D005894:Panax / Q000254:growth & development / Q000382:microbiology; D010802:Phylogeny; D012988:Soil Microbiology
chemical_list: D036145:Ginsenosides