title: Maternal high-fat diet programs for metabolic disturbances in offspring despite leptin sensitivity.
abstract: A fatty diet during pregnancy in mouse dams causes metabolic abnormalities (similar to metabolic syndrome in humans) in the rodents' offspring. We tested the hypothesis that the offspring of dams fed a high-fat diet during pregnancy and lactation develop metabolic abnormalities and leptin resistance. Pregnant C57BL/6 mice (n = 20) were fed either standard chow (SC; 19% fat) or a high-fat diet (HF; 49% fat). After weaning, male offspring were divided into four groups, according to the diet of dams and offspring: SC(dams)/SC(offspring), SC/HF, HF/SC and HF/HF (n = 30/group). For a metabolic analysis, we evaluated body mass, fat mass depots, blood plasma and adipocyte structure at 12 weeks of age. To analyse leptin sensitivity, each group was divided into two groups (vehicle or leptin) to identify the feeding response and pSTAT3 expression after acute intracerebroventricular (ICV) treatment. The offspring of mothers fed a high-fat diet presented increased body mass and visceral fat, adipocyte hypertrophy and insulin resistance. This phenotype was not associated with central leptin resistance. Thus, maternal programming by HF predisposes offspring to metabolic abnormalities despite leptin sensitivity.
authors.affiliation: Laboratory of Morphometry, Biomedical Center, Institute of Biology, State University of Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Volpato
authors.forename: Alini
authors.initials: A
authors.lastname: Schultz
authors.forename: Eduardo
authors.initials: E
authors.lastname: Magalhães-da-Costa
authors.forename: Marcelo Lima de Gusmão
authors.initials: ML
authors.lastname: Correia
authors.forename: Márcia Barbosa
authors.initials: MB
authors.lastname: Águila
authors.forename: Carlos Alberto
authors.initials: CA
authors.lastname: Mandarim-de-Lacerda
mesh_terms: D050154:Adiposity / Q000502:physiology*; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D059305:Diet, High-Fat / Q000009:adverse effects* / Q000379:methods; D004041:Dietary Fats / Q000008:administration & dosage / Q000009:adverse effects; D005260:Female; D007333:Insulin Resistance / Q000502:physiology*; D020738:Leptin / Q000097:blood*; D008297:Male; D039382:Maternal Nutritional Physiological Phenomena / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010641:Phenotype; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000097:blood*
chemical_list: D001786:Blood Glucose; D004041:Dietary Fats; D020738:Leptin