title: Crystallization and preliminary X-ray analysis of alginate importer from Sphingomonas sp. A1.
abstract: Sphingomonas sp. A1 directly incorporates alginate polysaccharides through a 'superchannel' comprising a pit on the cell surface, alginate-binding proteins in the periplasm and an ABC transporter (alginate importer) in the inner membrane. Alginate importer, consisting of four subunits, AlgM1, AlgM2 and two molecules of AlgS, was crystallized in the presence of the binding protein AlgQ2. Preliminary X-ray analysis showed that the crystal diffracted to 3.3 Å resolution and belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 72.5, b = 136.8, c = 273.3 Å, suggesting the presence of one complex in the asymmetric unit.
authors.affiliation: Laboratory of Basic and Applied Molecular Biotechnology, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan.
authors.forename: Yukie
authors.initials: Y
authors.lastname: Maruyama
authors.forename: Takafumi
authors.initials: T
authors.lastname: Itoh
authors.forename: Yu
authors.initials: Y
authors.lastname: Nishitani
authors.forename: Bunzo
authors.initials: B
authors.lastname: Mikami
authors.forename: Wataru
authors.initials: W
authors.lastname: Hashimoto
authors.forename: Kousaku
authors.initials: K
authors.lastname: Murata
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D020578:Sphingomonas / Q000737:chemistry*
chemical_list: D018528:ATP-Binding Cassette Transporters