title: Effect of host portal and peripheral sera fractions on cell proliferation of Schistosoma mansoni schistosomules.
abstract: Schistosomules of Schistosoma mansoni were incubated in medium containing   hamster (highly susceptible host) portal or peripheral venous serum, or rat (poorly susceptible   host) portal or peripheral venous serum or their fractions in the presence of bromodeoxyuridine   (BrdU) in order to determine effects of host sera on cell proliferation. BrdU labeling indices   (BLIs) were significantly increased in the presence of portal (33.05±0.70, p<0.05), but not in   peripheral serum (19.1±0.85, p>0.05) of hamsters, compared to schistosomules cultured in   presence of control medium (18.96±0.66). This stimulatory effect was substantially reproduced   by fraction 4 (31.03±0.69, p>0.05) separated from hamster portal serum. In contrast, no   significant differences were observed in the BLIs in rat portal or peripheral sera as well as   their fractions when compared to control medium. Taken together, it was concluded that   portal serum of hamster and its fraction which includes a low molecular weight protein (20   kDa) enhanced cell proliferation of S. mansoni schistosomules. This could explain the   localization and preference of S. mansoni schistosomules and worms to portal-mesenteric   venous system.
authors.affiliation: Department of Biochemistry, National Research Centre, Dokki, Cairo, Egypt.
authors.forename: Y M
authors.initials: YM
authors.lastname: Shaker
authors.forename: M A
authors.initials: MA
authors.lastname: Hamdy
authors.forename: M
authors.initials: M
authors.lastname: Ismail
authors.forename: H M
authors.initials: HM
authors.lastname: Draz
authors.forename: E
authors.initials: E
authors.lastname: Ashour
authors.forename: W
authors.initials: W
authors.lastname: Gouda
mesh_terms: D000818:Animals; D006224:Cricetinae; D003470:Culture Media / Q000737:chemistry; D006133:Growth Substances / Q000378:metabolism; D051381:Rats; D015203:Reproducibility of Results; D012550:Schistosoma mansoni / Q000254:growth & development* / Q000378:metabolism; D044967:Serum / Q000378:metabolism*
chemical_list: D003470:Culture Media; D006133:Growth Substances