title: [Expression of BNIP3 and its correlations to HIF-1alpha and VEGF in clear cell renal cell carcinoma].
abstract: OBJECTIVE
  To study the expressions of proapoptosis protein BNIP3 and its correlation with HIF-1alpha and VEGF in clear cell renal cell carcinoma (ccRCC).
  
  
  METHODS
  The expression levels of BNIP3, HIF-1alpha and VEGF were examined by two-step immunohistochemical staining with tissue chip technique in 104 cases of ccRCC and in 48 cases of normal renal tissues. The correlation of BNIP3 expression with HIF-1alpha and VEGF was analyzed.
  
  
  RESULTS
  The positive expression rates of BNIP3, HIF-1alpha and VEGF were 36.5%, 61.5%, and 69.2% in ccRCC, while were 12.5%, 8.3%, and 12.5% in paracancerous normal renal tissue, respectively. The expression of both HIF-1alpha and VEGF were not significantly increased in BNIP3 positive tumors in comparison with BNIP3 negative counterpart (P > 0.05), but there was a significant correlation between HIF-1alpha and VEGF (P < 0.05).
  
  
  CONCLUSIONS
  The lower expression level of BNIP3 is not coincident with the high level of HIF-1alpha and VEGF in clear cell renal cell carcinoma.
authors.affiliation: Department of Urology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Luo
authors.forename: Zi-Bing
authors.initials: ZB
authors.lastname: Xiong
authors.forename: Hao
authors.initials: H
authors.lastname: Zeng
authors.forename: Ni
authors.initials: N
authors.lastname: Chen
authors.forename: Xue-Qin
authors.initials: XQ
authors.lastname: Chen
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
mesh_terms: D017209:Apoptosis / Q000502:physiology; D002292:Carcinoma, Renal Cell / Q000378:metabolism*; D005260:Female; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D007680:Kidney Neoplasms / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: C090121:BNIP3 protein, human; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D008565:Membrane Proteins; D011518:Proto-Oncogene Proteins; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A