title: Pre-treatment with mesenchymal stem cells reduces ventilator-induced lung injury.
abstract: Bone marrow-derived mesenchymal stem cells (MSCs) reduce acute lung injury in animals challenged by bleomycin or bacterial lipopolysaccaride. It is not known, however, whether MSCs protect from ventilator-induced lung injury (VILI). This study investigated whether MSCs have a potential role in preventing or modulating VILI in healthy rats subjected to high-volume ventilation. 24 Sprague-Dawley rats (250-300 g) were subjected to high-volume mechanical ventilation (25 mL·kg(-1)). MSCs (5 × 10(6)) were intravenously or intratracheally administered (n=8 each) 30 min before starting over-ventilation and eight rats were MSC-untreated. Spontaneously breathing anesthetised rats (n=8) served as controls. After 3 h of over-ventilation or control the animals were sacrificed and lung tissue and bronchoalveolar lavage fluid (BALF) were sampled for further analysis. When compared with controls, MSC-untreated over-ventilated rats exhibited typical VILI features. Lung oedema, histological lung injury index, concentrations of total protein, interleukin-1β, macrophage inflammatory protein-2 and number of neutrophils in BALF and vascular cell adhesion protein-1 in lung tissue significantly increased in over-ventilated rats. All these indices of VILI moved significantly towards normalisation in the rats treated with MSCs, whether intravenously or intratracheally. Both local and systemic pre-treatment with MSCs reduced VILI in a rat model.
authors.affiliation: Unitat de Biofísica i Bioenginyeria, Facultat de Medicina, Casanova 143, 08036 Barcelona, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Chimenti
authors.forename: Tomás
authors.initials: T
authors.lastname: Luque
authors.forename: Maria R
authors.initials: MR
authors.lastname: Bonsignore
authors.forename: José
authors.initials: J
authors.lastname: Ramírez
authors.forename: Daniel
authors.initials: D
authors.lastname: Navajas
authors.forename: Ramon
authors.initials: R
authors.lastname: Farré
mesh_terms: D061605:Administration, Intravenous; D000287:Administration, Topical; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid; D004195:Disease Models, Animal; D018836:Inflammation Mediators / Q000378:metabolism; D008168:Lung / Q000378:metabolism / Q000473:pathology; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D011654:Pulmonary Edema / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism; D055397:Ventilator-Induced Lung Injury / Q000150:complications / Q000473:pathology / Q000517:prevention & control*
chemical_list: D018836:Inflammation Mediators; D019010:Vascular Cell Adhesion Molecule-1