title: [Development of in vitro digestion model for assessing the bioaccessibility of deoxynivalenol derived from foods].
abstract: OBJECTIVE
  To develop and validate in vitro digestion model for assessing the bioaccessibility of deoxynivalenol (DON) derived from foods, and optimize the major parameters affecting the model.
  
  
  METHODS
  To study the effect of digestion time, the fasting and feeding status, the volume and the pH of digestive juice, as well as other food components ingested with DON derived from mixed foodstuff on the bioaccessiblity of DON in a model simulating the process manner on gastrointestinal physiological digestion. To optimize some technical parameters of the model and validate it with both mycotoxin adsorbents and foods naturally contaminated by DON.
  
  
  RESULTS
  The optimized conditions of which DON released from the ingested foods at the highest concentration in gastrointestinal tract are as follows: digestion of 6 min, 1.5h and 1.5h in mouth, stomach and duodenum, respectively. At pH value of 6.5, 0.9, 7.5 and 8.0 as well as digestive juice volume of 6 ml, 13 ml, 11 ml and 6 ml for saliva, gastric juice, intestinal juice and bile, respectively. The bioaccessibility of DON at fasting and different feeding status was in the range from 81.28% to 99.58%, and the bioaccessibility of DON was reduced with the increase of contents in stomach. No significant effect of food matrix type (corn or wheat) on the bioaccessibility of DON was observed. However, other food components consumed along with DON can reduce the bioaccessibility of DON in cereals.
  
  
  CONCLUSIONS
  The in vitro digestion model developed for assessing the bioaccessibility of DON is stable and reproducible, which can meet the requirement of studies on the bioaccessibility of DON derived from foods.
authors.affiliation: Institute of Nutrition and Food Safety, China CDC, Beijing 100050, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Lin
authors.forename: Bing
authors.initials: B
authors.lastname: Shao
authors.forename: Fengqin
authors.initials: F
authors.lastname: Li
mesh_terms: D000327:Adsorption; D004063:Digestion; D004068:Digestive System Physiological Phenomena; D005506:Food Contamination; D041981:Gastrointestinal Tract / Q000502:physiology*; D008954:Models, Biological; D014255:Trichothecenes / Q000493:pharmacokinetics* / Q000633:toxicity
chemical_list: D014255:Trichothecenes; C007262:deoxynivalenol