title: Quality of cellular attachment to various root-end filling materials.
abstract: OBJECTIVE
  This study investigated cellular attachment to 6 root-end filling materials as a measure of the biocompatibility of the materials.
  
  
  METHODS
  Class I retrograde cavities were prepared in root slices and these cavities were filled with the test materials, and incubated with Balb/C 3T3 fibroblasts for 24 h. Root slices with the cavities left empty served as the controls. The root slices were then processed for scanning electron microscopy, and were viewed to assess the quality of cellular attachment by observing the shape of cells, spread, and membrane outline.
  
  
  RESULTS
  The best cellular attachment was observed at MTA and Geristore surfaces: cells exhibited characteristic elongated fibroblastic morphology, with projections of lamellipodia, filopodia, blebs, and microvilli from their surfaces, reflecting good attachment to the material. Fibroblasts attached poorly to the surfaces of IRM, Super EBA, KetacFil and Retroplast. Furthermore, the cells did not attach well to the tooth structure next to IRM and Super EBA.
  
  
  CONCLUSIONS
  The present study demonstrated a variation in cellular attachment to different root-end filling materials with the best cellular attachment to the surfaces of MTA and Geristore. IRM and Super EBA, KetacFil and Retroplast rendered poor attachment.
authors.affiliation: Department of Conservative Dentistry, Faculty of Dentistry, Jordan University of Science and Technology, Irbid, Jordan. hiyasat@just.edu.jo
authors.forename: Ahmad S
authors.initials: AS
authors.lastname: Al-Hiyasat
authors.forename: Oula R
authors.initials: OR
authors.lastname: Al-Sa'Eed
authors.forename: Homa
authors.initials: H
authors.lastname: Darmani
mesh_terms: D017607:Aluminum Compounds; D000818:Animals; D041702:BALB 3T3 Cells; D001672:Biocompatible Materials; D017438:Bisphenol A-Glycidyl Methacrylate; D017610:Calcium Compounds; D002448:Cell Adhesion / Q000502:physiology; D003764:Dental Materials; D004338:Drug Combinations; D005347:Fibroblasts / Q000502:physiology*; D005899:Glass Ionomer Cements; D008422:Materials Testing; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010087:Oxides; D012117:Resins, Synthetic; D012387:Root Canal Filling Materials; D017640:Silicates; D013997:Time Factors
chemical_list: D017607:Aluminum Compounds; D001672:Biocompatible Materials; D017610:Calcium Compounds; D003764:Dental Materials; D004338:Drug Combinations; C074933:Geristore; D005899:Glass Ionomer Cements; D010087:Oxides; D012117:Resins, Synthetic; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate; D017438:Bisphenol A-Glycidyl Methacrylate