title: Optimising Pichia pastoris induction.
abstract: A common method for inducing the production of recombinant proteins in Pichia pastoris is through the use of methanol. However, the by-products of methanol metabolism are toxic to yeast cells and therefore its addition to recombinant cultures must be controlled and monitored throughout the process in order to maximise recombinant protein yields. Described here are online and off-line methods to monitor and control methanol addition to bench-top-scale bioreactors.
authors.affiliation: School of Life & Health Sciences and Aston Research Centre for Healthy Ageing, Aston University, Birmingham, UK. bawaz@aston.ac.uk
authors.forename: Zharain
authors.initials: Z
authors.lastname: Bawa
authors.forename: Richard A J
authors.initials: RA
authors.lastname: Darby
mesh_terms: D019149:Bioreactors / Q000382:microbiology*; D005285:Fermentation; D000432:Methanol / Q000009:adverse effects / Q000378:metabolism; D010843:Pichia / Q000187:drug effects / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D000432:Methanol