title: Intravenous and intra-arterial administration of bone marrow mononuclear cells after focal cerebral ischemia: Is there a difference in biodistribution and efficacy?
abstract: Intravascular delivery of cells has been increasingly used in stroke models and clinical trials. We compared the biodistribution and therapeutic effects of bone marrow mononuclear cells (BMMCs) delivered by intra-arterial (IA) or intravenous (IV) injection after cortical ischemia. For the biodistribution analyses, BMMCs were labeled with (99m)Technetium ((99m)Tc). At 2 h, gamma-well counting of the brain and of the other organs evaluated did not show differences between the non-ischemic and ischemic groups or between injection routes, and the organs with the highest uptake were the liver and lungs, with low uptake in the brain. At 24 h, the liver maintained the highest activity, and a marked decrease was seen in pulmonary uptake in all groups. At this time point, although the activity in the brain remained low, the lesioned hemisphere showed greater homing than the contralateral hemisphere, for both the IV and IA ischemic groups. Histological analysis by CellTrace labeling indicated similar homing between both routes in the peri-infarct region 24 h after transplantation and functional recovery was observed in both groups up to 11 weeks after the lesion. In conclusion, transplantation of BMMCs by IA or IV routes may lead to similar brain homing and therapeutic efficacy after experimental stroke.
authors.affiliation: Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. andreia@biof.ufrj.br
authors.forename: Andréia
authors.initials: A
authors.lastname: Vasconcelos-dos-Santos
authors.forename: Paulo Henrique
authors.initials: PH
authors.lastname: Rosado-de-Castro
authors.forename: Sergio Augusto
authors.initials: SA
authors.lastname: Lopes de Souza
authors.forename: Juliana
authors.initials: J
authors.lastname: da Costa Silva
authors.forename: Alane Bernardo
authors.initials: AB
authors.lastname: Ramos
authors.forename: Gabriel
authors.initials: G
authors.lastname: Rodriguez de Freitas
authors.forename: Lea Mirian
authors.initials: LM
authors.lastname: Barbosa da Fonseca
authors.forename: Bianca
authors.initials: B
authors.lastname: Gutfilen
authors.forename: Rosalia
authors.initials: R
authors.lastname: Mendez-Otero
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation / Q000379:methods*; D002545:Brain Ischemia / Q000628:therapy*; D007269:Injections, Intra-Arterial; D007275:Injections, Intravenous; D008297:Male; D009000:Monocytes / Q000166:cytology*; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution; D016896:Treatment Outcome