title: The effects of acetazolamide on ischemia reperfused isolated hearts of 2- and 8-week-old rabbits.
abstract: OBJECTIVE
  To investigate the effects of acetazolamide on the ischemia-reperfused isolated hearts of 2- and 8-week-old rabbits.
  
  
  METHODS
  This study was conducted at the Kermanshah Medical Biology Research Center, Kermanshah, Iran from March to September 2011. Two- (n=17) and 8-week old (n=17) rabbits were separately divided into 2 control (n=9), and test (n=8) groups. Isolated hearts were subjected to 35 minutes ischemia and 30 minutes reperfusion. Acetazolamide (100 microgr/l) was added to the perfusion solution for 10 minutes before ischemia in the test group. Cardiac parameters including ventricular pressure, heart rate (HR), and rate pressure product (RPP) were measured. Data sets were analyzed by t-test.
  
  
  RESULTS
  Following acetazolamide administration the change percentage of HR was significantly different in the 2-week (91 +/- 1.1%) compared with the 8-week (96  +/- 0.8%) test groups (p=0.0016). Recovery percentage of RPP in reperfusion was lower (p=0.005) in the 8- (28.9  +/- 3.4%) than the 2-week test groups (45.2  +/- 3.5%).
  
  
  CONCLUSIONS
  The 2-week hearts elicited more rapid response to carbonic anhydrase (CA) inhibition than the 8-week group. However, acetazolamide does not exacerbate ischemia-reperfusion (I/R) injury in the 2-week hearts. Therefore, it was revealed that after inhibition of CA, the age dependent pattern of I/R injury was similar to that of the normal hearts. Inspite of the CA important role in the normal heart function, it is not a determining factor in I/R injury in different ages.
authors.affiliation: Department of Pediatrics, Kermanshah Medical Biology Research Center, Kermanshah, Iran.
authors.forename: Sohyla
authors.initials: S
authors.lastname: Reshadat
authors.forename: Rojin
authors.initials: R
authors.lastname: Nikray
authors.forename: Shirin
authors.initials: S
authors.lastname: Alord
authors.forename: Dareuosh
authors.initials: D
authors.lastname: Shackebaei
authors.forename: Aliashraf
authors.initials: A
authors.lastname: Godini
authors.forename: Mahvash
authors.initials: M
authors.lastname: Hesari
mesh_terms: D000086:Acetazolamide / Q000627:therapeutic use*; D000818:Animals; D016022:Case-Control Studies; D066298:In Vitro Techniques; D017202:Myocardial Ischemia / Q000503:physiopathology*; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control*; D011817:Rabbits
chemical_list: D000086:Acetazolamide