title: Molecular cloning and characterization of interleukin-1β in half-smooth tongue sole Cynoglossus semilaevis.
abstract: As a member of the interleukin (IL)-1 family, IL-1β is a prototypical proinflammatory cytokine, which plays a crucial role in immune responses. Herein, we reported the full length cDNA of IL-1β in half-smooth tongue sole (Cynoglossus semilaevis). The csIL-1β cDNA contained a 130 bp 5' UTR, a 417 bp 3' UTR and a 741 bp coding sequence (CDS) that translated into a polypeptide of 246 amino acids. The protein sequence included a typical IL-1 family signature and lacked an interleukin-converting enzyme (ICE) cut site. RT-PCR analysis indicated a broad expression of csIL-1β, especially in immune-related organs. After injection with inactive Vibrio anguillarum, the expression of csIL-1β was induced in the head kidney and spleen and reached the highest level at 8 h post injection. Higher expression of csIL-1β was observed at gastrula stage, eye-bud stage and hatching stage and lower level of csIL-1β mRNA was detected at metamorphic stage. The expression of csIL-1β during development suggested IL-1β might be involved not only in immunity but also development. Taken together, the present study indicated that csIL-1β played an important role in immune responses and development like its mammalian counterparts, although species-specific features were present.
authors.affiliation: Department of Life Science, Huainan Normal University, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yu
authors.forename: Qiwang
authors.initials: Q
authors.lastname: Zhong
authors.forename: Chunmei
authors.initials: C
authors.lastname: Li
authors.forename: Liming
authors.initials: L
authors.lastname: Jiang
authors.forename: Yeying
authors.initials: Y
authors.lastname: Sun
authors.forename: Xubo
authors.initials: X
authors.lastname: Wang
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Wang
authors.forename: Quanqi
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D005412:Flatfishes / Q000235:genetics* / Q000276:immunology*; D018507:Gene Expression Regulation, Developmental; D060226:Head Kidney / Q000276:immunology / Q000821:virology; D053583:Interleukin-1beta / Q000235:genetics* / Q000276:immunology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D013154:Spleen / Q000276:immunology / Q000821:virology; D014735:Vibrio Infections / Q000276:immunology / Q000662:veterinary / Q000821:virology
chemical_list: D053583:Interleukin-1beta; D012333:RNA, Messenger