title: [Adipose tissue sample preparation for scanning electron microscopy].
abstract: Conventional sample preparation technique for adipose tissue is difficult to achieve satisfactory results for scanning electron microscopy (SEM). We adopted a strategy of postfix with osmium tetroxide to stabilize the fatty acids, phospholipids protein, and hence the membrane structure. Also by extending the dehydration time to fully replace the organic solvents, we achieved satisfactory results for SEM of adipose samples.
authors.affiliation: Laboratory Center of Electron Microscopy, Hebei Medical University, Shijiazhuang 050017, China. fanghua1980@hotmail.com
authors.forename: Fang
authors.initials: F
authors.lastname: Cui
authors.forename: Chao
authors.initials: C
authors.lastname: Liu
authors.forename: Jian-Min
authors.initials: JM
authors.lastname: Yang
authors.forename: Li
authors.initials: L
authors.lastname: Wang
mesh_terms: D000273:Adipose Tissue / Q000648:ultrastructure*; D000818:Animals; D005260:Female; D006801:Humans; D008855:Microscopy, Electron, Scanning; D051381:Rats; D017207:Rats, Sprague-Dawley; D013048:Specimen Handling / Q000379:methods*