title: Comparison of synthetic oviductal fluid and G1/G2 medium under low-1 oxygen atmosphere on embryo production and pregnancy rates in Nelore (Bos indicus) cattle.
abstract: In this work, we evaluated whether embryo development and pregnancy rates would be affected by culturing bovine Bos indicus embryos in Synthetic Oviductal Fluid with amino acids (SOFaa) or G1/G2 sequential medium under a low-oxygen atmosphere. Using Ovum Pick Up, we obtained 1,538 oocytes, divided into G1/G2 (n = 783) and SOFaa (n = 755). No difference was observed for blastocyst development among the groups (27.8% ± 14.6 and 34.9% ± 20.0 for G1/G2 and SOFaa respectively, p > 0.05). Transferring the embryos (n = 450) from both groups to recipients resulted in similar pregnancy rates for the G1/G2 (38.4% n = 78/203) compared to the SOFaa (39.7% n = 98/247). Our findings confirm that Bos indicus embryos cultured in SOFaa and G1/G2 under low-oxygen atmosphere have similar in vitro (blastocyst rate) and in vivo (pregnancy rate) developmental capacity. However, embryos cultured in G1/G2 medium have higher cleavage than those cultured in SOFaa medium.
authors.affiliation: In Vitro Brasil Ltda Research Division, Mogi Mirim, SP, Brazil.
authors.forename: B V
authors.initials: BV
authors.lastname: Sanches
authors.forename: J H F
authors.initials: JH
authors.lastname: Pontes
authors.forename: A C
authors.initials: AC
authors.lastname: Basso
authors.forename: C R
authors.initials: CR
authors.lastname: Ferreira
authors.forename: F
authors.initials: F
authors.lastname: Perecin
authors.forename: M M
authors.initials: MM
authors.lastname: Seneda
mesh_terms: D000818:Animals; D001826:Body Fluids / Q000737:chemistry*; D002417:Cattle / Q000196:embryology* / Q000502:physiology; D003470:Culture Media / Q000737:chemistry*; D046149:Embryo Culture Techniques / Q000662:veterinary*; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D009865:Oocytes / Q000166:cytology / Q000502:physiology; D010057:Oviducts / Q000502:physiology*; D010100:Oxygen; D011247:Pregnancy
chemical_list: D003470:Culture Media; D010100:Oxygen