title: Expression of PRB, FKBP52 and HB-EGF relating with ultrasonic evaluation of endometrial receptivity.
abstract: BACKGROUND
  To explore the molecular basis of the different ultrasonic patterns of the human endometrium, and the molecular marker basis of local injury.
  
  
  RESULTS
  The mRNA and protein expression of FKBP52, progesterone receptor A (PRA), progesterone receptor B (PRB), and HB-EGF were detected in different patterns of the endometrium by real-time RTPCR and immunohistochemistry. There were differences in the mRNA and protein expression of FKBP52, PRB, and HB-EGF in the triple line (Pattern A) and homogeneous (Pattern C) endometrium in the window of implantation. No difference was detected in PRA expression. After local injury, the mRNA expression of HB-EGF significantly increased. In contrast, there was no difference in the mRNA expression of FKBP52, PRB, or PRA. The protein expression of FKBP52, PRB, and HB-EGF increased after local injury. There was no difference in the PRA expression after local injury.
  
  
  CONCLUSIONS
  PRB, FKBP52, and HB-EGF may be the molecular basis for the classification of the ultrasonic patterns. HB-EGF may be the molecular basis of local injury. Ultrasonic evaluation on the day of ovulation can be effective in predicting the outcome of implantation.
authors.affiliation: National Research Institute for Family Planning, Beijing, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.forename: Linlin
authors.initials: L
authors.lastname: Geng
authors.forename: Shucheng
authors.initials: S
authors.lastname: Zhang
authors.forename: Bin
authors.initials: B
authors.lastname: He
authors.forename: Jiedong
authors.initials: J
authors.lastname: Wang
mesh_terms: D000328:Adult; D004717:Endometrium / Q000000981:diagnostic imaging* / Q000293:injuries / Q000378:metabolism*; D005260:Female; D066257:Heparin-binding EGF-like Growth Factor; D006801:Humans; D007124:Immunoenzyme Techniques; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055273:Salivary Proline-Rich Proteins / Q000235:genetics / Q000378:metabolism*; D022021:Tacrolimus Binding Proteins / Q000235:genetics / Q000378:metabolism*; D014463:Ultrasonography
chemical_list: C587883:HBEGF protein, human; D066257:Heparin-binding EGF-like Growth Factor; D036341:Intercellular Signaling Peptides and Proteins; C524744:PRB1 protein, human; D012333:RNA, Messenger; D055273:Salivary Proline-Rich Proteins; D022021:Tacrolimus Binding Proteins; C403485:tacrolimus binding protein 4