title: Characterization of the uterine leiomyoma microRNAome by deep sequencing.
abstract: MicroRNAs (miRNAs) are a class of small non-coding RNAs, which are negative regulators of gene expression. Many genes in human uterine leiomyoma (ULM) are aberrantly expressed and in some cases this can be due to dysregulation of miRNAs. Here we present the first study to determine genome-wide miRNA expression patterns in uterine leiomyoma and myometrium using Solexa high-throughput sequencing. We found more than 50 miRNAs, which were differentially expressed, and furthermore we extend the list of putative new miRNA genes. The top five significantly de-regulated miRNAs in ULMs that we found in our libraries were miR-363, miR-490, miR-137, miR-217 and miR-4792. We also observed "isomiRs" with higher copy number than referenced mature miRNA specific for the leiomyoma libraries, which have a potential role in tumorigenesis. The microRNA transcriptomes obtained in this study deliver insights and further expand our understanding the role of small RNAs in uterine leiomyoma development.
authors.affiliation: Department of Plant Physiology and Molecular Biology, University of Plovdiv, 24 TsarAssen St, 4000 Plovdiv, Bulgaria.
authors.forename: Boryana
authors.initials: B
authors.lastname: Georgieva
authors.forename: Ivan
authors.initials: I
authors.lastname: Milev
authors.forename: Ivan
authors.initials: I
authors.lastname: Minkov
authors.forename: Irina
authors.initials: I
authors.lastname: Dimitrova
authors.forename: Andrew P
authors.initials: AP
authors.lastname: Bradford
authors.forename: Vesselin
authors.initials: V
authors.lastname: Baev
mesh_terms: D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D015723:Gene Library; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D007889:Leiomyoma / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA / Q000379:methods; D012689:Sequence Homology, Nucleic Acid; D059467:Transcriptome; D014594:Uterine Neoplasms / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; C545567:MIRN137 microRNA, human; C553315:MIRN217 microRNA, human; C574861:MIRN363 microRNA, human; C574863:MIRN4792 microRNA, human; C574862:MIRN490 microRNA, human; D035683:MicroRNAs