title: Influence of survivin and Bcl-2 expression on the biological behavior             of non-small cell lung cancer.
abstract: Survivin and Bcl-2 are generally considered to be inhibitors of apoptosis             and are frequently overexpressed in several types of human cancers. However, their             role in regulating the biological behavior of non-small cell lung carcinoma (NSCLC)             remains controversial. We aimed to determine the expression of survivin and Bcl-2             and explore their correlation with clinicopathological features and prognosis.             The expression of survivin and Bcl-2 proteins in 62 specimens of NSCLC tissues             and 30 specimens of tumor adjacent tissues was detected using immunohistochemistry.             The correlation between protein expression and clinicopathological features and             prognosis was analyzed. The percentage of survivin-positive samples obtained from             NSCLC tissues was 58.06% (36/62), which was significantly higher compared to that             in normal lung tissues (10%, 3/30; P<0.05). Similarly, the percentage of Bcl-2-positive             samples obtained from NSCLC tissues was statistically higher compared to that             from normal lung tissues (51.61%, 32/62 vs. 6.67%, 2/30; P<0.05). Survivin             expression was closely correlated with tumor differentiation, lymph node metastasis             and TNM stage (P<0.05), while Bcl-2 expression was only associated with TNM             stage (P<0.05). The expression of survivin was positively correlated with that             of Bcl-2 (P<0.05). A five-year follow-up study revealed that the expression             of survivin and Bcl-2 was negatively correlated with post-operative survival duration.             Our findings suggest that survivin and Bcl-2 may act synergistically in the occurrence,             development, invasion and metastasis of NSCLC, both of which are up-regulated             in NSCLC tissues. The co-expression of survivin and Bcl-2, which is closely related             to malignancy, may serve as a biomarker for predicting prognosis.
authors.affiliation: Department of Thoracic Surgery, The Union Hospital of Fujian Medical University, Fujian, People's Republic of China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Gao
authors.forename: Sheng
authors.initials: S
authors.lastname: Yang
authors.forename: Ming-Qiang
authors.initials: MQ
authors.lastname: Kang
mesh_terms: D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D005260:Female; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics / Q000378:metabolism*; D053208:Kaplan-Meier Estimate; D008175:Lung Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism*; D000077022:Survivin
chemical_list: C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D000077022:Survivin