title: Five years' experience of classical swine fever polymerase chain reaction ring trials in France.
abstract: Since 2004, the French National Reference Laboratory for classical swine fever (CSF) has conducted an annual proficiency test (PT) to evaluate the ability of local veterinary laboratories to perform real-time polymerase chain reaction (PCR) for CSF virus. The results of five years of testing (2004-2008) are described here. The PT was conducted under blind conditions on 20 samples. The same batch of samples was used for all five years. The number of laboratories that analysed the samples increased from four in 2004 to 13 in 2008. The results of the PT showed the following: cross-contamination between samples and deficiencies in RNA preparation can occur even in experienced laboratories; sample homogeneity should be checked carefully before selection; samples stored at-80 degrees C for several years remain stable; and poor shipment conditions do not damage the samples with regard to detection of CSF virus genome. These results will enable redesign of the panel to improve the overall quality of the PT, which will encourage laboratories to check and improve their PCR procedures and expertise. This is an excellent way to determine laboratory performance.
authors.affiliation: Anses, Ploufragan/Plouzané Laboratory, Swine Immunology and Virology Unit, B.P. 53, 22440 Ploufragan, France. francoise.pol@anses.fr
authors.forename: F
authors.initials: F
authors.lastname: Po
authors.forename: M
authors.initials: M
authors.lastname: Le Dimna
authors.forename: M F
authors.initials: MF
authors.lastname: Le Potier
mesh_terms: D000818:Animals; D006691:Classical Swine Fever / Q000175:diagnosis* / Q000821:virology; D006692:Classical Swine Fever Virus / Q000235:genetics / Q000302:isolation & purification*; D018076:DNA, Complementary / Q000032:analysis; D005602:France; D007753:Laboratories / Q000592:standards*; D011786:Quality Control; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D060888:Real-Time Polymerase Chain Reaction / Q000592:standards / Q000662:veterinary*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013154:Spleen / Q000821:virology; D013552:Swine; D014730:Veterinary Medicine / Q000592:standards*
chemical_list: D018076:DNA, Complementary; D012367:RNA, Viral