title: Optimization of pulsed-field gel electrophoresis protocols for Salmonella Paratyphi A subtyping.
abstract: Salmonella enterica serovar Paratyphi A infection has caused public health problems in some countries in recent years. Pulsed-field gel electrophoresis (PFGE) has been used for the subtyping and epidemiological investigations of some serotypes of Salmonella, mainly in outbreaks caused by non-typhoidal Salmonella. In this study, different restriction endonucleases and electrophoresis parameters were compared for the PFGE subtyping by using Salmonella Paratyphi A strain panels. Two protocols for the enzymes SpeI and XbaI showed higher discriminatory power, which may facilitate epidemiological analysis for more accurate case definition, and clonality study of Salmonella Paratyphi A.
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 155 Changbai Road, Beijing, China.
authors.forename: Chunxia
authors.initials: C
authors.lastname: Chen
authors.forename: Yingwei
authors.initials: Y
authors.lastname: Zhao
authors.forename: Hui
authors.initials: H
authors.lastname: Han
authors.forename: Bo
authors.initials: B
authors.lastname: Pang
authors.forename: Jingyun
authors.initials: J
authors.lastname: Zhang
authors.forename: Meiying
authors.initials: M
authors.lastname: Yan
authors.forename: Baowei
authors.initials: B
authors.lastname: Diao
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Cui
authors.forename: Haijian
authors.initials: H
authors.lastname: Zhou
authors.forename: Weili
authors.initials: W
authors.lastname: Liang
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Feng
authors.forename: Biao
authors.initials: B
authors.lastname: Kan
mesh_terms: D015373:Bacterial Typing Techniques; D002681:China / Q000453:epidemiology; D016000:Cluster Analysis; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific; D004196:Disease Outbreaks / Q000145:classification*; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods* / Q000592:standards; D010284:Paratyphoid Fever / Q000453:epidemiology / Q000382:microbiology*; D010865:Pilot Projects; D011634:Public Health; D012482:Salmonella paratyphi A / Q000145:classification* / Q000235:genetics; D013997:Time Factors
chemical_list: D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes; C056436:endodeoxyribonuclease SpeI; C025580:endodeoxyribonuclease XBAI; D015252:Deoxyribonucleases, Type II Site-Specific