title: Non-invasive genotyping of transgenic animals using fecal DNA.
abstract: For genotyping of transgenic animals, many IACUC guidelines recommend the use of fecal DNA when possible because this approach is non-invasive. Existing methods for extracting fecal DNA may be costly or involve the use of toxic organic solvents. Furthermore, feces contain an abundance of PCR inhibitors that may hinder DNA amplification when they are co-purified with fecal DNA. Here the authors describe a cost-effective, non-toxic method for genotyping transgenic animals by using the reagent AquaStool to extract fecal DNA and remove PCR inhibitors. Genotyping results obtained from fecal DNA samples extracted using AquaStool were reliably accurate when compared with results obtained from tail DNA samples. Because it is non-invasive, the authors believe that use of this method for genotyping transgenic animals using fecal DNA samples may improve animal welfare.
authors.affiliation: MultiTarget Pharmaceuticals LLC, Salt Lake City, UT, USA. zchen@multitargetpharm.com
authors.forename: Zhidong
authors.initials: Z
authors.lastname: Chen
authors.forename: Rebecca R
authors.initials: RR
authors.lastname: Mantha
authors.forename: Janice S
authors.initials: JS
authors.lastname: Chen
authors.forename: Orazio J
authors.initials: OJ
authors.lastname: Slivano
authors.forename: Hideko
authors.initials: H
authors.lastname: Takahashi
mesh_terms: D035661:Animal Care Committees; D000827:Animal Welfare; D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics*; D005591:Chemical Fractionation / Q000379:methods*; D004247:DNA / Q000032:analysis / Q000235:genetics*; D005243:Feces / Q000737:chemistry*; D005838:Genotype; D012680:Sensitivity and Specificity
chemical_list: D004247:DNA