title: Effects of a natural prolyl oligopeptidase inhibitor, rosmarinic acid, on lipopolysaccharide-induced acute lung injury in mice.
abstract: Rosmarinic acid (RA), a polyphenolic phytochemical, is a natural prolyl oligopeptidase inhibitor. In the present study, we found that RA exerted potent anti-inflammatory effects in in vivo models of acute lung injury (ALI) induced by lipopolysaccharide (LPS). Mice were pretreated with RA one hour before challenge with a dose of 0.5 mg/kg LPS. Twenty-four hours after LPS was given, bronchoalveolar lavage fluid (BALF) was obtained to measure pro-inflammatory mediator and total cell counts. RA significantly decreased the production of LPS-induced TNF-a, IL-6, and IL-1β compare with the LPS group. When pretreated with RA (5, 10, or 20 mg/kg) the lung wet-to-dry weight (W/D) ratio of the lung tissue and the number of total cells, neutrophils and macrophages in the BALF were decreased significantly. Furthermore, RA may enhance oxidase dimutase (SOD) activity during the inflammatory response to LPS-induced ALI. And we further demonstrated that RA exerts anti-inflammation effect in vivo models of ALI through suppresses ERK/MAPK signaling in a dose dependent manner. These studies have important implications for RA administration as a potential treatment for ALI.
authors.affiliation: College of Animal Science and Technology, Guangxi University, 100 Daxue Road, Nanning 530005, Guangxi, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Chu
authors.forename: Xinxin
authors.initials: X
authors.lastname: Ci
authors.forename: Jiakang
authors.initials: J
authors.lastname: He
authors.forename: Lanxiang
authors.initials: L
authors.lastname: Jiang
authors.forename: Miaomiao
authors.initials: M
authors.lastname: Wei
authors.forename: Qingjun
authors.initials: Q
authors.lastname: Cao
authors.forename: Mingfeng
authors.initials: M
authors.lastname: Guan
authors.forename: Xianxing
authors.initials: X
authors.lastname: Xie
authors.forename: Xuming
authors.initials: X
authors.lastname: Deng
authors.forename: Jiakang
authors.initials: J
authors.lastname: He
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced* / Q000188:drug therapy* / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D002934:Cinnamates / Q000737:chemistry / Q000627:therapeutic use*; D053630:Depsides / Q000737:chemistry / Q000627:therapeutic use*; D004195:Disease Models, Animal; D004791:Enzyme Inhibitors / Q000627:therapeutic use*; D008070:Lipopolysaccharides / Q000633:toxicity*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D000085822:Prolyl Oligopeptidases; D011480:Protease Inhibitors / Q000737:chemistry / Q000627:therapeutic use*; D012697:Serine Endopeptidases / Q000378:metabolism*; D000097123:Rosmarinic Acid
chemical_list: D002934:Cinnamates; D053630:Depsides; D004791:Enzyme Inhibitors; D008070:Lipopolysaccharides; D000085822:Prolyl Oligopeptidases; D011480:Protease Inhibitors; D012697:Serine Endopeptidases; D000097123:Rosmarinic Acid