title: [Immunophenotyping and its clinical significance in childhood acute lymphoblastic leukemia].
abstract: OBJECTIVE
  To study the immunophenotype and its relationship with clinical characteristics in children with acute lymphoblastic leukemia (ALL).
  
  
  METHODS
  Bone marrow or blood samples (2-3 mL) with heparin anticoagulation from 139 children with ALL were obtained, and immunophenotypes were identified by flow cytometry.
  
  
  RESULTS
  In 139 ALL children, there were 103 cases (74.1%) of B-ALL, 24 cases (17.3%) of T-ALL, 12 cases of T/B biphenotypic (8.6% of T/BALL). In the 103 children with B-ALL, CD19 (90.3%), CD10 (83.5%) and CD20 (27.2%) were expressed as major antigens. In the 24 children with T-ALL, the major antigens were CD3 (79.2%), CD7 (66.7%) and CD5 (33.3%). In the 12 children with B/T-ALL, T-lymphoid antigens included CD7 (50.0%) and CD5 (41.7%), while the B-lymphoid antigens included CD19 (50.0%) and CD10 (33.3%). Of the 139 children with ALL, 32 cases (23.0%) showed myeloid antigen expression (My+ ALL) and the main expression antigens were CD13, CD33, CD14 and MPO. CD34 was expressed in 31 cases. CD34-positive expression (15.6%) in My+ ALL children was significantly lower than in My-ALL children (24.3%). HLA-DR was expressed in 82 of the 139 ALL children. The expression of CD10, CD34 and HLA-DR in the standard-risk, medium risk, high-risk ALL children was significantly different. There were significant differences in gender and incidence of bleeding between the My+ ALL and My-ALL groups (P<0.05).
  
  
  CONCLUSIONS
  Immunetyping can differentiate the sources of leukemic cells. The expression of CD10, CD34 and HLA-DR antigen is related to the clinical classification of ALL.
authors.affiliation: Zhengzhou Children's Hospital, Zhengzhou 450053, China.
authors.forename: Yao-Dong
authors.initials: YD
authors.lastname: Zhang
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Tan
authors.forename: Qun
authors.initials: Q
authors.lastname: Hu
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Wei
authors.forename: Xiao-Ling
authors.initials: XL
authors.lastname: Zhang
authors.forename: Hao
authors.initials: H
authors.lastname: Xiong
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D005260:Female; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D016130:Immunophenotyping; D007223:Infant; D008297:Male; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000276:immunology*
chemical_list: D006684:HLA-DR Antigens