title: Nerve cell response to inhibitors recorded with an aluminum-galliumnitride/galliumnitride field-effect transistor.
abstract: Experiments based on neuronal cell-transistor couplings were made from some groups during the last years. Pioneering work in this field was carried out by Fromherz and his group (Fromherz, 2003; Schmidtner and Fromherz, 2006). We were interested of the interaction of nerve cells to serine hydrolase inhibitor diisopropylfluorophosphate (DFP), monitored by using an aluminum-galliumnitride/galliumnitride (AlGaN/GaN) electrolyte gate field effect transistor (EGFET). The biocompatibility study of our sensor materials with nerve cells shows a proliferation rate of at least 95%. The inhibitors were added to the medium and the source-drain current of the EGFET was recorded as a function of time. The inhibitor was added to the NG108-15 nerve cells growing directly on the sensor surface, resulting in a fast decrease in the drain current, I(DS). Control measurements show that this response is associated with cationic fluxes pumped through ionic channels present in the cellular membrane. The sensor enables analysis of the ion channel activity without cell destruction and simultaneously allows visual observation due to the optical transparency of the sensor material.
authors.affiliation: ZIK MacroNano Microfluidics and Biosensors, Ilmenau University of Technology, P.O. Box 100565, D-98684 Ilmenau, Germany. michael.gebinoga@tu-ilmenau.de
authors.forename: Michael
authors.initials: M
authors.lastname: Gebinoga
authors.forename: Patrick
authors.initials: P
authors.lastname: Mai
authors.forename: Mary
authors.initials: M
authors.lastname: Donahue
authors.forename: Mario
authors.initials: M
authors.lastname: Kittler
authors.forename: Irina
authors.initials: I
authors.lastname: Cimalla
authors.forename: Benedikt
authors.initials: B
authors.lastname: Lübbers
authors.forename: Maren
authors.initials: M
authors.lastname: Klett
authors.forename: Vadim
authors.initials: V
authors.lastname: Lebedev
authors.forename: Liele
authors.initials: L
authors.lastname: Silveira
authors.forename: Sukhdeep
authors.initials: S
authors.lastname: Singh
authors.forename: Andreas
authors.initials: A
authors.lastname: Schober
mesh_terms: D017607:Aluminum Compounds; D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002460:Cell Line; D004594:Electrophysiology / Q000379:methods; D005708:Gallium; D007531:Isoflurophate / Q000494:pharmacology; D051379:Mice; D009474:Neurons / Q000187:drug effects*; D051381:Rats; D014173:Transistors, Electronic
chemical_list: D017607:Aluminum Compounds; C513700:aluminum gallium nitride; D007531:Isoflurophate; C473348:gallium nitride; D005708:Gallium