title: Parvovirus B19 is a bystander in adult myocarditis.
abstract: BACKGROUND
  The genomic DNA of parvovirus B19, a small single-stranded DNA virus of the genus Erythrovirus, has been shown to persist in solid tissues of constitutionally healthy, immunocompetent individuals. Despite these data, many case reports and series have linked the presence of parvovirus B19 genomic DNA, detected through nucleic acid amplification testing, with myocarditis and cardiomyopathy. Herein, we use multiple tools to better assess the relationship between parvovirus B19 and myocarditis and cardiomyopathy.
  
  
  METHODS
  Nucleic acid amplification testing, immunohistochemistry, in situ hybridization, and electron microscopy were used to assess the location and activity of parvovirus B19 in cases of myocarditis and in cases with no significant cardiac disease.
  
  
  RESULTS
  Nucleic acid amplification testing for parvovirus B19 genomic DNA was positive in 73% of patients with myocarditis/cardiomyopathy and in 26% of patients with no significant disease. In situ hybridization and immunohistochemistry showed that, in cases with amplifiable parvovirus B19 DNA, parvovirus B19 genomic DNA and viral protein production were present in rare mononuclear cells.
  
  
  CONCLUSIONS
  In a majority of cases of myocarditis and a significant number of otherwise normal hearts, nucleic acid amplification testing detected persistent parvovirus B19 genomic DNA that did not play a significant pathogenic role. The source of parvovirus B19 DNA appeared to be interstitial mononuclear inflammatory cells and not myocardial or endothelial cells. Therefore, nucleic acid amplification testing alone is not diagnostically helpful for determining the etiology of adult myocarditis.
authors.affiliation: Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE, USA.
authors.forename: Scott A
authors.initials: SA
authors.lastname: Koepsell
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Anderson
authors.forename: Stanley J
authors.initials: SJ
authors.lastname: Radio
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D024201:Bystander Effect; D002648:Child; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006321:Heart / Q000821:virology; D054884:Host-Pathogen Interactions; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007963:Leukocytes, Mononuclear / Q000473:pathology / Q000821:virology; D008297:Male; D046529:Microscopy, Electron, Transmission; D008875:Middle Aged; D009205:Myocarditis / Q000473:pathology / Q000821:virology*; D009206:Myocardium / Q000473:pathology; D010322:Parvoviridae Infections / Q000150:complications* / Q000473:pathology; D016732:Parvovirus B19, Human / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity* / Q000648:ultrastructure; D016133:Polymerase Chain Reaction; D055815:Young Adult
chemical_list: D004279:DNA, Viral