title: Development of microsatellite markers in Fosterella rusbyi (Bromeliaceae) using 454 pyrosequencing.
abstract: OBJECTIVE
  Polymorphic microsatellite markers were developed for Fosterella rusbyi (Bromeliaceae) to evaluate the population genetic structure and genetic diversity of natural populations of F. rusbyi and other Fosterella species in Bolivia.
  
  
  RESULTS
  454 pyrosequencing technology was used to generate 73027 sequence reads from F. rusbyi DNA, which together contained 2796 perfect simple sequence repeats (SSRs). Primer pairs were designed for 30 loci, of which 15 were used to genotype 30 F. rusbyi plants from two geographical areas in Bolivia. All markers were polymorphic, with two to nine alleles in the overall sample. Cross-species amplification was tested in 10 additional Fosterella species. Seven loci showed consistent amplification in six or more species.
  
  
  CONCLUSIONS
  The 15 SSR markers developed for F. rusbyi are promising candidates for population genetic analyses within F. rusbyi and other species of Fosterella.
authors.affiliation: Plant Molecular Systematics, Department of Sciences, University of Kassel, Kassel, Germany.
authors.forename: Tina
authors.initials: T
authors.lastname: Wöhrmann
authors.forename: Natascha
authors.initials: N
authors.lastname: Wagner
authors.forename: Florian
authors.initials: F
authors.lastname: Krapp
authors.forename: Bruno
authors.initials: B
authors.lastname: Huettel
authors.forename: Kurt
authors.initials: K
authors.lastname: Weising
mesh_terms: D031229:Bromeliaceae / Q000235:genetics*; D017931:DNA Primers / Q000378:metabolism; D056426:Genetic Loci / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA / Q000379:methods*; D013696:Temperature
chemical_list: D017931:DNA Primers