title: Gene expression profile of Campylobacter jejuni-induced GBS in Bama miniature pigs.
abstract: Our aim was to investigate the in vivo gene expression pattern of the Guillain-Barre syndrome (GBS) with DNA microarrays and bioinformatics tools. Oral-infusion model animals mimicking human infection of GBS were analyzed. Tissue samples and body fluids were collected to perform antibody tests and biopsy assays. Gene-expression microarray was conducted with nerve tissues and GBS-related genes were elucidated via bioinformatics tools. Model animals showed typical symptoms of GBS in that mild demyelination was shown by cerebellar white matter and by lumbar enlargement of model animals. Then, 81.25% of the model animals were positive with GM1-IgG antibodies by ELISA. In the microarray analysis, 1,261 genes were identified with statistically different expression (P < 0.05), 21 of which were associated with gene function analysis, gene pathway identification, signal transduction and co-expression network construction. Furthermore, quantitative PCR was used to characterize the gene expression level. We found that genes of HPRT1, PKC and PPARGC-1 were in the core of the network, while the expression of PPARGC-1, SUS2DD and AMPKA2 were significantly inhibited. A total of 21 genes were found to be actively involved in the process of protein transportation, transcriptional regulation, antigen identification and cell cycle regulation during the GBS infection period. The co-expression network indicated an important association between GBS and the 21 genes, especially the down-regulated ones. In conclusion, we demonstrated that GBS-affected hosts had a specific gene expression profile, which may guide the direction of GBS research and therapy.
authors.affiliation: APFIC, Jiangsu Entry-Exit Inspection and Quarantine Bureau, Nanjing, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Xue
authors.forename: Dexin
authors.initials: D
authors.lastname: Zeng
authors.forename: Fei
authors.initials: F
authors.lastname: Xu
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.forename: Yang
authors.initials: Y
authors.lastname: Chen
authors.forename: Yuefei
authors.initials: Y
authors.lastname: Yang
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Wang
authors.forename: Zhenzhong
authors.initials: Z
authors.lastname: Li
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Zhang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D001706:Biopsy; D016123:Campylobacter jejuni / Q000502:physiology*; D005260:Female; D005677:G(M1) Ganglioside / Q000276:immunology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks / Q000235:genetics; D020275:Guillain-Barre Syndrome / Q000235:genetics / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D006801:Humans; D007118:Immunoassay; D007074:Immunoglobulin G / Q000276:immunology; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000235:genetics; D013194:Staining and Labeling; D013552:Swine; D013553:Swine Diseases / Q000235:genetics* / Q000382:microbiology*; D013556:Swine, Miniature / Q000235:genetics* / Q000382:microbiology*
chemical_list: D000906:Antibodies; D007074:Immunoglobulin G; D005677:G(M1) Ganglioside