title: Berry fruits modulated endothelial cell migration and angiogenesis via phosphoinositide-3 kinase/protein kinase B pathway in vitro in endothelial cells.
abstract: Polyphenolic-rich berry fruits are known to activate redox-sensitive cellular signaling molecules such as phosphatidylinositol-3-kinase (PI3 kinase)/kinase B (Akt), resulting in a cascade of downstream signaling pathways. This study investigated the ability of strawberry (SB), wild blueberry (WBB), and cranberry (CB) extracts to induce the activation of PI3 kinase/Akt signaling in vitro in human umbilical endothelial cells (HUVECs) and whether this activation would enhance cell migration and angiogenesis. Anthocyanin profiles of the extracts were characterized using HPLC-ESI/MS, and Akt activation was investigated using the Alpha Screen SureFire assay. The total anthocyanin contents of SB, WBB, and CB extracts were 81.7, 82.5, and 83.0 mg/100 g fresh weight, respectively. SB, WBB, and CB extracts activated Akt in a dose-dependent manner via PI3 kinase and induced cell migration and angiogenesis in vitro in HUVECs. The results from this study suggest that polyphenolics in berry fruits may play a role in promoting vascular health.
authors.affiliation: Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration , 6502 South Archer Road, Bedford Park, Illinois 60501, United States.
authors.forename: Artemio Z
authors.initials: AZ
authors.lastname: Tulio
authors.forename: Claire
authors.initials: C
authors.lastname: Chang
authors.forename: Indika
authors.initials: I
authors.lastname: Edirisinghe
authors.forename: Kevin D
authors.initials: KD
authors.lastname: White
authors.forename: Joseph E
authors.initials: JE
authors.lastname: Jablonski
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Banaszewski
authors.forename: Archana
authors.initials: A
authors.lastname: Kangath
authors.forename: Ravi K
authors.initials: RK
authors.lastname: Tadapaneni
authors.forename: Britt
authors.initials: B
authors.lastname: Burton-Freeman
authors.forename: Lauren S
authors.initials: LS
authors.lastname: Jackson
mesh_terms: D000872:Anthocyanins / Q000494:pharmacology; D002465:Cell Movement / Q000187:drug effects*; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D005638:Fruit / Q000737:chemistry*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D009389:Neovascularization, Pathologic; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology; D059808:Polyphenols / Q000494:pharmacology; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D000872:Anthocyanins; D010936:Plant Extracts; D059808:Polyphenols; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt