title: Advanced microscale bioreactor system: a representative scale-down model for bench-top bioreactors.
abstract: In recent years, several automated scale-down bioreactor systems have been developed to increase efficiency in cell culture process development. ambr™ is an automated workstation that provides individual monitoring and control of culture dissolved oxygen and pH in single-use, stirred-tank bioreactors at a working volume of 10-15 mL. To evaluate the ambr™ system, we compared the performance of four recombinant Chinese hamster ovary cell lines in a fed-batch process in parallel ambr™, 2-L bench-top bioreactors, and shake flasks. Cultures in ambr™ matched 2-L bioreactors in controlling the environment (temperature, dissolved oxygen, and pH) and in culture performance (growth, viability, glucose, lactate, Na(+), osmolality, titer, and product quality). However, cultures in shake flasks did not show comparable performance to the ambr™ and 2-L bioreactors.
authors.affiliation: Early Stage Cell Culture, Genentech, Inc., 1 DNA Way, South San Francisco, CA, 94080, USA, wendyh@gene.com.
authors.forename: Wei-Ting
authors.initials: WT
authors.lastname: Hsu
authors.forename: Rigzen P S
authors.initials: RP
authors.lastname: Aulakh
authors.forename: Donald L
authors.initials: DL
authors.lastname: Traul
authors.forename: Inn H
authors.initials: IH
authors.lastname: Yuk