title: Macroscale and microscale analysis of Anammox in anaerobic rotating biological contactor.
abstract: Inoculated with conventional anaerobic activated sludge, the Anammox process was successfully developed in an anaerobic rotating biological contactor (AnRBC) fed with a low ratio of C/N synthetic wastewater. Operated in a single point feed mode, the AnRBC removed 92.1% (n = 126) of the influent N at the highest surface load of 12 g/(m2 x day). The biomass increased by 25% and 17.1 g/(m2 x day) of maximum N removal surface load was achieved by elevating flow rate with another feed point. Fluorescence in situ hybridization and polymerase chain reaction analysis indicated that the Anammox genus Candidatus Kuenenia stuttgartiensis dominated the community. Both Anammox and denitrifying activity were detected in biofilm by the application of microelectrodes. In the outer layer of the biofilm (0-2500 microm), nitrite and ammonium consumed simultaneously in a ratio of 1.12/1, revealing the occurrence of Anammox. In the inner layer (> 2500 microm), a decrease of nitrate was caused by denitrification in the absence of nitrite and ammonium.
authors.affiliation: School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China. hybos2000@126.com
authors.forename: Yongtao
authors.initials: Y
authors.lastname: Lv
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Xudong
authors.initials: X
authors.lastname: Wang
authors.forename: Yongzhe
authors.initials: Y
authors.lastname: Yang
authors.forename: Zhiying
authors.initials: Z
authors.lastname: Wang
authors.forename: Jie
authors.initials: J
authors.lastname: Li
mesh_terms: D000641:Ammonia / Q000737:chemistry* / Q000378:metabolism; D000693:Anaerobiosis; D018533:Biomass; D019149:Bioreactors / Q000592:standards*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D058440:Denitrification; D017404:In Situ Hybridization, Fluorescence; D009584:Nitrogen / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D016133:Polymerase Chain Reaction; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D014874:Water Pollutants, Chemical; D000641:Ammonia; D009584:Nitrogen