title: An unusual taxodione derivative from hairy roots of Salvia austriaca.
abstract: From a root culture of Salvia austriaca, transformed with Agrobacterium rhizogenes, a new diterpenoid was isolated and its chemical structure was determined as 7-(2-oxohexyl)-11-hydroxy-6, 12-dioxo-7,9(11),13- abietatriene [= 7-(2-oxohexyl)-taxodione] on the basis of spectroscopic methods, especially 1D and 2D NMR, and by comparison with structurally related compounds. This compound represents a hitherto unknown 2-oxohexyl diterpenoid derivative. Cytotoxic studies revealed that the new compound exhibited high cytotoxic activity against three cancer cell lines with IC(50) values ranging from 0.63 to 0.72μM. Its cytotoxic effectiveness against the cancer cells was ten fold higher than that of taxodione.
authors.affiliation: Department of Biology and Pharmaceutical Botany, Medical University of Łódź, Muszyńskiego 1, Pl- 90-151 Łódź, Poland.
authors.forename: Lukasz
authors.initials: L
authors.lastname: Kuźma
authors.forename: Halina
authors.initials: H
authors.lastname: Wysokińska
authors.forename: Marek
authors.initials: M
authors.lastname: Różalski
authors.forename: Urszula
authors.initials: U
authors.lastname: Krajewska
authors.forename: Wanda
authors.initials: W
authors.lastname: Kisiel
mesh_terms: D060054:Agrobacterium; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D002460:Cell Line; D045744:Cell Line, Tumor; D004224:Diterpenes / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D006801:Humans; D020128:Inhibitory Concentration 50; D015394:Molecular Structure; D009369:Neoplasms / Q000188:drug therapy*; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D018517:Plant Roots / Q000737:chemistry; D027544:Salvia / Q000737:chemistry*
chemical_list: C575170:7-(2-oxohexyl)-11-hydroxy-6,12-dioxo-7,9(11),13-abietatriene; D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes; D010936:Plant Extracts