title: Evaluation of sCMOS cameras for detection and localization of single Cy5 molecules.
abstract: The ability to detect single molecules over the electronic noise requires high performance detector systems. Electron Multiplying Charge-Coupled Device (EMCCD) cameras have been employed successfully to image single molecules. Recently, scientific Complementary Metal Oxide Semiconductor (sCMOS) based cameras have been introduced with very low read noise at faster read out rates, smaller pixel sizes and a lower price compared to EMCCD cameras. In this study, we have compared the two technologies using two EMCCD and three sCMOS cameras to detect single Cy5 molecules. Our findings indicate that the sCMOS cameras perform similar to EMCCD cameras for detecting and localizing single Cy5 molecules.
authors.affiliation: Department of Chemistry, Carnegie Mellon University, 4400 Fifth Ave, Pittsburgh, Pennsylvania 15213, USA.
authors.forename: Saumya
authors.initials: S
authors.lastname: Saurabh
authors.forename: Suvrajit
authors.initials: S
authors.lastname: Maji
authors.forename: Marcel P
authors.initials: MP
authors.lastname: Bruchez
mesh_terms: D002232:Carbocyanines / Q000032:analysis*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005456:Fluorescent Dyes / Q000032:analysis; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D057054:Molecular Imaging / Q000295:instrumentation*; D015336:Molecular Probe Techniques / Q000295:instrumentation*; D012666:Semiconductors
chemical_list: D002232:Carbocyanines; D005456:Fluorescent Dyes; C085321:cyanine dye 5