title: A simplified PCR-based method for detection of gray coat color allele in horse.
abstract: Coat color of gray horses is associated with a 4.6-kb duplication, which can be determined using PCR amplification of about 5-kb DNA fragment. In practice, this means that amplification might fail frequently. Therefore, a novel genetic screening method based on amplification of the 246 bp DNA fragment has been developed.
authors.affiliation: Department of Animal Science, Biotechnical Faculty, University of Ljubljana, SI-1230 Domžale, Groblje 3, Slovenia. tatjana.kavar@bf.uni-lj.si
authors.forename: Tatjana
authors.initials: T
authors.lastname: Kavar
authors.forename: Eva
authors.initials: E
authors.lastname: Čeh
authors.forename: Peter
authors.initials: P
authors.lastname: Dovč
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D020440:Gene Duplication; D005799:Genes, Dominant; D005820:Genetic Testing; D060005:Genotyping Techniques; D006200:Hair Color / Q000235:genetics*; D006736:Horses / Q000235:genetics* / Q000502:physiology; D016133:Polymerase Chain Reaction; D050765:Qa-SNARE Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D050765:Qa-SNARE Proteins