title: [Improvement of 2-mercaptoimidazoline analysis in rubber products containing chlorine].
abstract: An improved analysis method for 2-mercaptoimidazoline in rubber products containing chlorine was developed. 2-Mercaptoimidazoline (20 µg/mL) is detected by means of TLC with two developing solvents in the official method. But, this method is not quantitative. Instead, we employed HPLC using water-methanol (9 : 1) as the mobile phase. This procedure decreased interfering peaks, and the quantitation limit was 2 µg/mL of standard solution. 2-Mercaptoimidazoline was confirmed by GC-MS (5 µg/mL) and LC/MS (1 µg/mL) in the scan mode. For preparation of test solution, a soaking extraction method, in which 20 mL of methanol was added to the sample and allowed to stand overnight at about 40°C, was used. This gave similar values to the Soxhlet extraction method (official method) and was more convenient. The results indicate that our procedure is suitable for analysis of 2-mercaptoimidazoline. When 2-mercaptoimidazoline is detected, it is confirmed by either GC/MS or LC/MS.
authors.affiliation: Tokyo Metropolitan Institute of Public Health, Tokyo, Japan. Reiko_Kaneko@member.metro.tokyo.jp
authors.forename: Reiko
authors.initials: R
authors.lastname: Kaneko
authors.forename: Nahoko
authors.initials: N
authors.lastname: Haneishi
authors.forename: Yoko
authors.initials: Y
authors.lastname: Kawamura
mesh_terms: D002713:Chlorine / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005031:Ethylenethiourea / Q000032:analysis*; D012408:Rubber / Q000032:analysis*
chemical_list: D005031:Ethylenethiourea; D002713:Chlorine; D012408:Rubber