title: An SSH library responsive to azadirachtin A constructed in Spodoptera litura Fabricius cell lines.
abstract: The present study revealed differentially expressed genes responsive to azadirachtin A (Aza) in Spodoptera litura cell line through suppression subtractive hybridization. In the Aza-responsive SSH library, approximately 270 sequences represent 53 different identified genes encoding proteins with various predicted functions, and the percentages of the gene clusters were 26.09% (genetic information processing), 11.41% (cell growth and death), 7.07% (metabolism), 6.52% (signal transduction/transport) and 2.72% (immunity), respectively. Eleven clones homologous to identified genes were selected to be confirmed through quantitative real time polymerase chain reaction. Among the eleven clones validated, all but one transcript of lipase showed an increase in SL cell line collected from ETA, whereas the transcripts of other genes were lower in the SL cell line collected from ETA compared with that of UETA. These genes were considered to be related to the response of SL cell line to Aza. These will provide a new clue to uncover the molecular mechanisms of Aza acting on SL cell line.
authors.affiliation: Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, Laboratory of Insect Toxicology, South China Agricultural University, Guangzhou, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Yan
authors.forename: Zhi-Xiang
authors.initials: ZX
authors.lastname: Zhang
authors.forename: Han-Hong
authors.initials: HH
authors.lastname: Xu
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D003598:Cytoskeletal Proteins / Q000378:metabolism; D030541:Databases, Genetic; D004305:Dose-Response Relationship, Drug; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D015723:Gene Library; D017344:Genes, Insect / Q000235:genetics; D006360:Heat-Shock Proteins / Q000378:metabolism; D019476:Insect Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism; D036701:Limonins / Q000494:pharmacology*; D009693:Nucleic Acid Hybridization / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D018411:Spodoptera / Q000166:cytology / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism
chemical_list: D003598:Cytoskeletal Proteins; D006360:Heat-Shock Proteins; D019476:Insect Proteins; D036701:Limonins; C010329:azadirachtin