title: Effect of copper chloride exposure on the membrane potential and cytosolic free calcium in primary cultured chicken hepatocytes.
abstract: This study was conducted to examine the effects of copper on membrane potential and cytosolic free calcium in isolated primary chicken hepatocytes which were exposed to different concentration of Cu(2+) (0, 10, 50, 100 μM) or a mixture of Cu(2+) and vitamin C (50 and 50 μM, respectively). Viability, membrane potential, and cytosolic free Ca(2+) of monolayer cultured hepatocytes were investigated at the indicated time point. Results showed that, among the different concentrations of Cu(2+) exposure, the viability of hepatocytes treated with 100 μM Cu(2+) was the worst at the 12th and 24th hours. The effects of Cu(2+) on viability and proliferation were time and dose dependent. Further investigation indicated that Cu(2+) exposure significantly enhanced cytosolic free Ca(2+) in hepatocytes, compared to that in control group, at the 24th hour. Meanwhile, membrane potential was noticeably reduced in hepatocytes increasing concentration of Cu(2+). Taking these results together, we have shown that Cu(2+) can cause toxicity to primary chicken hepatocytes in excessive dose and the effect of Cu(2+) exposure on membrane potential is not site specific, which is probably mediated by the changes of cytosolic free Ca(2+).
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Xuexia
authors.initials: X
authors.lastname: Jia
authors.forename: Long
authors.initials: L
authors.lastname: Chen
authors.forename: Jingtao
authors.initials: J
authors.lastname: Li
authors.forename: Rongsheng
authors.initials: R
authors.lastname: Su
authors.forename: Dayou
authors.initials: D
authors.lastname: Shi
authors.forename: Zhaoxin
authors.initials: Z
authors.lastname: Tang
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D002645:Chickens; D003300:Copper / Q000494:pharmacology*; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism*; D008564:Membrane Potentials / Q000187:drug effects*
chemical_list: D003300:Copper; D002118:Calcium