title: MiR-128 inhibits tumor growth and angiogenesis by targeting p70S6K1.
abstract: MicroRNAs are a class of small noncoding RNAs that function as critical gene regulators through targeting mRNAs for translational repression or degradation. In this study, we showed that miR-128 expression levels were decreased in glioma, and identified p70S6K1 as a novel direct target of miR-128. Overexpression of miR-128 suppressed p70S6K1 and its downstream signaling molecules such as HIF-1 and VEGF expression, and attenuated cell proliferation, tumor growth and angiogenesis. Forced expression of p70S6K1 can partly rescue the inhibitory effect of miR-128 in the cells. Taken together, these findings will shed light to the role and mechanism of miR-128 in regulating glioma tumor angiogenesis via miR-128/p70S6K1 axis, and miR-128 may serve as a potential therapeutic target in glioma in the future.
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Zhu-mei
authors.initials: ZM
authors.lastname: Shi
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Yan
authors.forename: Yong-ping
authors.initials: YP
authors.lastname: You
authors.forename: Chong-yong
authors.initials: CY
authors.lastname: Li
authors.forename: Xu
authors.initials: X
authors.lastname: Qian
authors.forename: Yu
authors.initials: Y
authors.lastname: Yin
authors.forename: Peng
authors.initials: P
authors.lastname: Zhao
authors.forename: Ying-ying
authors.initials: YY
authors.lastname: Wang
authors.forename: Xie-feng
authors.initials: XF
authors.lastname: Wang
authors.forename: Ming-na
authors.initials: MN
authors.lastname: Li
authors.forename: Ling-Zhi
authors.initials: LZ
authors.lastname: Liu
authors.forename: Ning
authors.initials: N
authors.lastname: Liu
authors.forename: Bing-Hua
authors.initials: BH
authors.lastname: Jiang
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D005910:Glioma / Q000201:enzymology* / Q000235:genetics / Q000628:therapy; D006801:Humans; D051793:Hypoxia-Inducible Factor 1 / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D009368:Neoplasm Transplantation; D009389:Neovascularization, Pathologic / Q000201:enzymology* / Q000235:genetics / Q000628:therapy; D038762:Ribosomal Protein S6 Kinases, 70-kDa / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics; D014183:Transplantation, Heterologous; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis / Q000235:genetics
chemical_list: D051793:Hypoxia-Inducible Factor 1; C545189:MIRN128 microRNA, human; D035683:MicroRNAs; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D038762:Ribosomal Protein S6 Kinases, 70-kDa