title: Modulation of the blood-stimulating effect of immobilized granulocyte colony-stimulating factor by immobilized hyaluronidase.
abstract: We evaluated whether immobilized hyaluronidase can modify the hematotropic effect of immobilized granulocyte CSF (G-CSF). The preparation of immobilized hyaluronidase (50 arb. units per mouse) potentiated the specific effect of immobilized G-CSF on granulomonocytopoiesis. The preparation was shown to facilitate the indirect effect of immobilized G-CSF on hemopoiesis (stimulation of the erythroid and lymphoid hemopoietic stems). These changes were accompanied by an increase in functional activity of hemopoietic precursor cells, secretion of humoral factors by bone marrow myelokaryocytes, and concentration of hemopoietins in the serum.
authors.affiliation: Institute of Pharmacology, Siberian Division of the Russian Academy of Medical Sciences, Tomsk, Russia. mmu@pharm.tsu.ru
authors.forename: A M
authors.initials: AM
authors.lastname: Dygai
authors.forename: V V
authors.initials: VV
authors.lastname: Zhdanov
authors.forename: G N
authors.initials: GN
authors.lastname: Zyuz'kov
authors.forename: E V
authors.initials: EV
authors.lastname: Udut
authors.forename: T Yu
authors.initials: TY
authors.lastname: Khrichkova
authors.forename: E V
authors.initials: EV
authors.lastname: Simanina
authors.forename: L A
authors.initials: LA
authors.lastname: Stavrova
authors.forename: L A
authors.initials: LA
authors.lastname: Miroshnichenko
authors.forename: A V
authors.initials: AV
authors.lastname: Chaikovskii
authors.forename: T S
authors.initials: TS
authors.lastname: Markova
authors.forename: R V
authors.initials: RV
authors.lastname: Gurto
authors.forename: A V
authors.initials: AV
authors.lastname: Artamonov
authors.forename: A A
authors.initials: AA
authors.lastname: Bekarev
authors.forename: P G
authors.initials: PG
authors.lastname: Madonov
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000187:drug effects; D001854:Bone Marrow Cells / Q000187:drug effects; D003520:Cyclophosphamide / Q000494:pharmacology; D004800:Enzymes, Immobilized; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D006098:Granulocytes / Q000187:drug effects*; D006410:Hematopoiesis / Q000187:drug effects*; D016298:Hematopoietic Cell Growth Factors / Q000097:blood; D006412:Hematopoietic Stem Cells / Q000187:drug effects; D006821:Hyaluronoglucosaminidase / Q000378:metabolism*; D051379:Mice
chemical_list: D004800:Enzymes, Immobilized; D016298:Hematopoietic Cell Growth Factors; D016179:Granulocyte Colony-Stimulating Factor; D003520:Cyclophosphamide; D006821:Hyaluronoglucosaminidase