title: Preliminary study on the effects of carbon dioxide and nitrogen pneumoperitoneums on endometriotic lesions.
abstract: OBJECTIVE
  To determine the effects of carbon dioxide (CO(2)) and nitrogen (N(2)) pneumoperitoneums on endometriosis (EMs) lesions.
  
  
  METHODS
  Female Wistar rats were randomized into the following 3 groups: CO(2) (N = 20), N(2) (N = 22) and air pneumoperitoneums (N = 9). After 5 weeks of establishment models, do the pneumoperitoneums. Then measure the size of EMs lesions and the related factors of serum and tissue after 1, 2, and 4 weeks of pneumoperitoneums.
  
  
  RESULTS
  (1) One week after the pneumoperitoneum was established, the EMs lesions in the CO(2) group were largest in volume, whereas at 4 weeks the EMs lesions in the CO(2) group were smaller than the N(2) group. (2) The level of ICAM-1 and TIMP-2 of serum in CO(2) and N(2) group after 2 weeks of pneumoperitoneum were higher than air group. (3) The expression of CD44v6, ICAM-1, MMP-2 and VEGF of tissue in CO(2) and N(2) group after 1, 2 and 4 weeks of pneumoperitoneum were lower than air group, TIMP-2 and ENS were higher than air group.
  
  
  CONCLUSIONS
  After a CO(2) pneumoperitoneum, EMs lesions were reduced in volume, suggesting an inhibitory effect on EMs lesions.
authors.affiliation: Department of Obstetrics and Gynecology, PLA General Hospital, Beijing 100853, China.
authors.forename: Lingyun
authors.initials: L
authors.lastname: Hu
authors.forename: Li-An
authors.initials: LA
authors.lastname: Li
authors.forename: Yali
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D002245:Carbon Dioxide / Q000627:therapeutic use*; D004715:Endometriosis / Q000628:therapy*; D043169:Endostatins / Q000096:biosynthesis; D005260:Female; D018960:Hyaluronan Receptors / Q000096:biosynthesis; D018799:Intercellular Adhesion Molecule-1 / Q000097:blood; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis; D009584:Nitrogen / Q000627:therapeutic use*; D011028:Pneumoperitoneum, Artificial / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000097:blood; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis
chemical_list: C112697:CD44v6 antigen; D043169:Endostatins; D018960:Hyaluronan Receptors; D042461:Vascular Endothelial Growth Factor A; C467498:vascular endothelial growth factor A, rat; D018799:Intercellular Adhesion Molecule-1; D019716:Tissue Inhibitor of Metalloproteinase-2; D002245:Carbon Dioxide; D020778:Matrix Metalloproteinase 2; C522363:Mmp2 protein, rat; D009584:Nitrogen