title: Differentiating between Burkitt lymphoma and CD10+ diffuse large B-cell lymphoma: the role of commonly used flow cytometry cell markers and the application of a multiparameter scoring system.
abstract: The goal of this study was to evaluate routine flow cytometric (FC) immunophenotypic markers in differentiating between Burkitt lymphoma (BL) and CD10+ diffuse large B-cell lymphoma (DLBCL). We performed retrospective analysis of FC data from 55 patients. We evaluated 9 FC parameters: forward and side scatter (FSC and SSC); mean fluorescent intensity (MFI) for CD20, CD10, CD38, CD79b, CD43, and CD71; and the percentage of neoplastic cells positive for CD71 (%CD71). The FSC; MFIs of CD10, CD43, CD79b, and CD71; and %CD71 cells were significantly different between BL and CD10+ DLBCL (P < .05; Student t test). A 5-point scoring system (FSC, %CD71, and MFIs of CD43, CD79b, and CD71) was devised, and 6 (60%) of 10 BLs scored 3 or greater and 1 (10%) of 10 CD10+ DLBCLs scored 3 (P = .04; χ(2)). Our findings indicate that routine FC parameters can aid in differentiating BL from CD10+ DLBCL.
authors.affiliation: Pathology and Laboratory Medicine, The Methodist Hospital, Houston, TX, USA.
authors.forename: Paul
authors.initials: P
authors.lastname: McGowan
authors.forename: Nicole
authors.initials: N
authors.lastname: Nelles
authors.forename: Jana
authors.initials: J
authors.lastname: Wimmer
authors.forename: Dawn
authors.initials: D
authors.lastname: Williams
authors.forename: Jianguo
authors.initials: J
authors.lastname: Wen
authors.forename: Marilyn
authors.initials: M
authors.lastname: Li
authors.forename: April
authors.initials: A
authors.lastname: Ewton
authors.forename: Choladda
authors.initials: C
authors.lastname: Curry
authors.forename: Youli
authors.initials: Y
authors.lastname: Zu
authors.forename: Andrea
authors.initials: A
authors.lastname: Sheehan
authors.forename: Chung-Che Jeff
authors.initials: CC
authors.lastname: Chang
mesh_terms: D000368:Aged; D002051:Burkitt Lymphoma / Q000175:diagnosis* / Q000276:immunology; D003937:Diagnosis, Differential; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D016403:Lymphoma, Large B-Cell, Diffuse / Q000175:diagnosis* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D015260:Neprilysin / Q000276:immunology; D012189:Retrospective Studies
chemical_list: D015260:Neprilysin