title: BMP2/7 heterodimer is a stronger inducer of bone regeneration in peri-implant bone defects model than BMP2 or BMP7 homodimer.
abstract: This study aimed to compare the effects of bone morphogenetic protein BMP2/7 heterodimer and BMP homodimers on bone regeneration in bone defects model. Identical peri-implant bone defects model were created using proper controls on the frontal skull in 18 minipigs. Collagen sponges with low-dose (30 ng/mL) BMP2/7 heterodimer, BMP2 or BMP7 homodimer were filled in the defects. New bone formation and the expression of type I collagen (Col1), alkaline phosphatase (ALP) and osteocalcin (OCN) were evaluated after 2, 3, and 6 weeks of implantation. BMP2/7 resulted in significantly higher new bone areas percentage in the defect region than BMP2 and BMP7 (p<0.05). Immunohistochemical staining of Col1, ALP and OCN was stronger in BMP2/7 group than BMP2, BMP7 and control group (p<0.05). These results demonstrate that BMP2/7 heterodimer is a stronger inducer of osteoblastogenesis and could be applied at low dose to reduce the cost and side effects of BMP homodimers.
authors.affiliation: School/Hospital of Stomatology, Zhejiang University, Hangzhou, Zhejiang, PR China.
authors.forename: Ping
authors.initials: P
authors.lastname: Sun
authors.forename: Jingxiao
authors.initials: J
authors.lastname: Wang
authors.forename: Yuanna
authors.initials: Y
authors.lastname: Zheng
authors.forename: Yi
authors.initials: Y
authors.lastname: Fan
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Gu
mesh_terms: D000469:Alkaline Phosphatase / Q000096:biosynthesis; D016301:Alveolar Bone Loss / Q000209:etiology / Q000601:surgery; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000008:administration & dosage / Q000737:chemistry* / Q000494:pharmacology; D055419:Bone Morphogenetic Protein 7 / Q000008:administration & dosage / Q000737:chemistry* / Q000494:pharmacology; D001861:Bone Regeneration / Q000187:drug effects*; D003094:Collagen; D024042:Collagen Type I / Q000096:biosynthesis; D015921:Dental Implants / Q000009:adverse effects; D019281:Dimerization; D004337:Drug Carriers; D005260:Female; D008297:Male; D010006:Osteoblasts / Q000378:metabolism; D015675:Osteocalcin / Q000096:biosynthesis; D057873:Peri-Implantitis / Q000209:etiology / Q000601:surgery*; D055503:Protein Multimerization; D011897:Random Allocation; D011994:Recombinant Proteins; D012886:Skull / Q000601:surgery; D013552:Swine; D013556:Swine, Miniature; D023822:Tissue Engineering
chemical_list: D055396:Bone Morphogenetic Protein 2; D055419:Bone Morphogenetic Protein 7; D024042:Collagen Type I; D015921:Dental Implants; D004337:Drug Carriers; D011994:Recombinant Proteins; D015675:Osteocalcin; D003094:Collagen; D000469:Alkaline Phosphatase