title: [Effect of fluoride on the expression of StAR mRNA and P450scc mRNA in the progesterone synthesis of mouse Leydig tumor cells].
abstract: OBJECTIVE
  To observe the effect of sodium fluoride on the expression of steroidogenic acute regulatory protein (StAR) mRNA and cytochrome P450 cholesterol side-chain cleavage enzyme (P450scc) mRNA in the testosterone synthesis of mouse Leydig tumor cells (mLTC-1). and to explore the molecular mechanisms of fluoride on reproductive injury.
  
  
  METHODS
  Using mLTC-1 cells as a model, progesterone in the supernatant of cell culture medium was measured by RIA. The expression of StAR mRNA and P450scc mRNA in mLTC-1 cells was determined by real-time PCR.
  
  
  RESULTS
  In comparison with the control group, the expression of StAR mRNA and P450scc mRNA in mLTC-1 cells and the secretion of progesterone of mLTC-1 cells in the three fluoride groups with 12,16 and 20 microg/ml of NaF in their media respectively were obviously lower (P < 0.05).
  
  
  CONCLUSIONS
  The expression of StAR mRNA and P450scc mRNA in mLTC-1 cells could be inhibited by NaF, and consequently the secretion of progesterone in mLTC-1 cells was affected.
authors.affiliation: Department of Environment Health, College of Public Health, Zhengzhou University, Zhengzhou 450001, China. guanyongbo602@sohu.com
authors.forename: Yongbo
authors.initials: Y
authors.lastname: Guan
authors.forename: Pengfei
authors.initials: P
authors.lastname: Hao
authors.forename: Chunyu
authors.initials: C
authors.lastname: Tang
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Shiqun
authors.initials: S
authors.lastname: Li
authors.forename: Xuemin
authors.initials: X
authors.lastname: Cheng
authors.forename: Yue
authors.initials: Y
authors.lastname: Ba
authors.forename: Liuxin
authors.initials: L
authors.lastname: Cui
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000235:genetics / Q000378:metabolism*; D007984:Leydig Cell Tumor / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051379:Mice; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D011374:Progesterone / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012969:Sodium Fluoride / Q000633:toxicity*; D013736:Testicular Neoplasms / Q000378:metabolism* / Q000473:pathology; D000098682:Steroidogenic Acute Regulatory Protein
chemical_list: D010750:Phosphoproteins; D012333:RNA, Messenger; D000098682:Steroidogenic Acute Regulatory Protein; D011374:Progesterone; D012969:Sodium Fluoride; D002786:Cholesterol Side-Chain Cleavage Enzyme