title: [Antagonistic activity of lactobacilli of the colon].
abstract: OBJECTIVE
  Study of antagonistic activity of lactobacilli of the colon against members of its autochthonous bacterial flora and agents of some acute infectious and chronic diseases of the gastrointestinal tract.
  
  
  METHODS
  Antagonistic activity of 19 lactobacilli cultures against 28 cultures of bacteria belonging to various groups and fungi was evaluated within the framework of specially developed two-stage cultivation technique in the conditions of a combined system. The results of the study were evaluated according to a semi-quantitative scale that allows to put one or the other value of the zone of growth delay of the studied strain culture in compliance with the one or the other (low, moderate, high) level of antagonistic activity of the lactobacillus culture.
  
  
  RESULTS
  Lactobacilli of the colon showed selective antagonistic activity against pathogenic enterobacteriae: pronounced against Salmonella enterica Serovar Enteritidis, Shigella flexneri 2b, Yersinia spp., and trace against Salmonella enterica Serovar Typhimurium. The level of antagonistic activity of lactobacilli against a wide range of members of autochthonous bacterial flora varied in a wide range, without revealing connection neither to its belonging to species, nor to its population level, nor to the belonging to group of the antagonistic effect objects. On the other hand a connection was traced with beloriging to a certain microbiota: being quite active against members of its own microbiota, lactobacilli often showed significantly lower level of antagonistic activity against cultures with the same species name isolated from other microbiota.
  
  
  CONCLUSIONS
  In light of the results obtained, level of lactobacilli population may hardly be viewed as the only criteria of their full participation in the process of stabilizing microecological welfare of the colon, that allows to make a complete representation of the level of dysbiotic disorder in the mentioned biotope. With in the framework of rational bacteriological diagnostics of the level of dysbiotic disorders in the colon, evaluation of population level of lactobacilli should be evaluated along with the degree of their antagonistic activity against other components of the same microbiota.
authors.forename: M A
authors.initials: MA
authors.lastname: Sukhina
authors.forename: O A
authors.initials: OA
authors.lastname: Burgasova
authors.forename: V G
authors.initials: VG
authors.lastname: Zhukhovitskiĭ
authors.forename: N D
authors.initials: ND
authors.lastname: Iushchuk
mesh_terms: D000898:Antibiosis; D058491:Bacterial Load / Q000187:drug effects; D015373:Bacterial Typing Techniques; D018929:Cell Culture Techniques; D003106:Colon / Q000382:microbiology*; D052978:Disk Diffusion Antimicrobial Tests; D006801:Humans; D007778:Lactobacillus / Q000378:metabolism*; D054892:Metagenome / Q000502:physiology*; D019936:Probiotics / Q000378:metabolism / Q000494:pharmacology*; D012477:Salmonella enteritidis / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification; D012486:Salmonella typhimurium / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification; D012763:Shigella flexneri / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification; D015007:Yersinia / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification