title: Differential WNT gene expression in various subtypes of acute lymphoblastic leukemia.
abstract: BACKGROUND
  Dysregulation of WNT signaling has been reported in many malignancies.
  
  
  OBJECTIVE
  This study was conducted to investigate the expression pattern of 14 members of the WNT gene family in different immunophenotypic subtypes of ALL.
  
  
  METHODS
  Semi-quantitative RT-PCR was performed on samples from 71 ALL patients and 36 age-matched healthy individuals. The ALL patients were categorized into B-ALL (76%), T-ALL (22.6%) and mixed lineage (1.4%) and the B-ALL cases were further classified into pro-B, pre-BI, pre-BII and immature/mature-B based on immuno-phenotypic results.
  
  
  RESULTS
  Among the WNT genes, WNT-7B (p=0.026), WNT-9A (p=0.020) and WNT-16B (p=0.023) were significantly over-expressed, whereas WNT-2B (p=0.033), WNT-5A (p=0.016), WNT-7A (p<0.0001) and WNT-10A (p<0.0001) were down-regulated in B-ALL. Among the T-ALL subtype, however, significant down-regulation of WNT-2B, WNT-5B, WNT-7A, WNT-10A and WNT-11 was evident. Comparison between B-ALL subtypes showed significant over-expression of WNT-7B, WNT-9A and WNT-5B in certain subtypes.
  
  
  CONCLUSIONS
  Our results suggest contribution of the WNT genes in leukemogenesis of ALL.
authors.affiliation: Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran, email: fshokri@sina.tums.ac.ir.
authors.forename: Ali
authors.initials: A
authors.lastname: Memarian
authors.forename: Parvaneh
authors.initials: P
authors.lastname: Vosough
authors.forename: Hossein
authors.initials: H
authors.lastname: Asgarian-Omran
authors.forename: Mina
authors.initials: M
authors.lastname: Tabrizi
authors.forename: Mahdi
authors.initials: M
authors.lastname: Shabani
authors.forename: Fazel
authors.initials: F
authors.lastname: Shokri
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D005434:Flow Cytometry; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D016130:Immunophenotyping; D007223:Infant; D015452:Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D054218:Precursor T-Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D020033:Protein Isoforms / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D059467:Transcriptome; D051153:Wnt Proteins / Q000235:genetics*; D055815:Young Adult
chemical_list: D020033:Protein Isoforms; D051153:Wnt Proteins