title: Regulation of inflammatory cytokines in lipopolysaccharide-stimulated RAW 264.7 murine macrophage by 7-O-methyl-naringenin.
abstract: 7-O-Methylnaringenin, extracted from Rhododendron speciferum, belongs to the flavanone class of polyphenols. In the present study, we investigated the anti-inflammatory effects of 7-O-methylnaringenin on cytokine production by lipopoly-saccharide (LPS)-stimulated RAW 264.7 macrophages in vitro. The results showed that pretreatment with 10, 20 or 40 μg/mL of 7-O-methylnaringenin could downregulate tumour necrosis factor (TNF-α), interleukin (IL-6) and interleukin (IL-1β) in a dose-dependent manner. Furthermore, we investigated the signal transduction mechanisms to determine how 7-O-methylnaringenin affects RAW 264.7 macrophages. The activation of mitogen-activated protein kinases (MAPK) and IκBα were measured by Western blotting. The data showed that 7-O-methylnaringenin could downregulate LPS-induced levels of phosphorylation of ERK1/2, JNK and IκBα. These observations indicated that 7-O-methylnaringenin modulated inflammatory cytokine responses by blocking NF-қB, ERK1/2 and JNK/MAPKs activation.
authors.affiliation: College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, Jilin, China. gshuang1973@126.com.
authors.forename: Lanan Wassy
authors.initials: LW
authors.lastname: Soromou
authors.forename: Zhichao
authors.initials: Z
authors.lastname: Zhang
authors.forename: Rongtao
authors.initials: R
authors.lastname: Li
authors.forename: Na
authors.initials: N
authors.lastname: Chen
authors.forename: Weixiao
authors.initials: W
authors.lastname: Guo
authors.forename: Meixia
authors.initials: M
authors.lastname: Huo
authors.forename: Shuang
authors.initials: S
authors.lastname: Guan
authors.forename: Jing
authors.initials: J
authors.lastname: Lu
authors.forename: Xuming
authors.initials: X
authors.lastname: Deng
mesh_terms: D000818:Animals; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism*; D044950:Flavanones / Q000737:chemistry / Q000494:pharmacology*; D053583:Interleukin-1beta / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism*; D051379:Mice; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D016207:Cytokines; D044950:Flavanones; D053583:Interleukin-1beta; D015850:Interleukin-6; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; C005273:naringenin