title: [Influence of Cx26/Cx32 gap junction channel on antineoplastic effect of etoposide in Hela cells].
abstract: OBJECTIVE
  To observe the influence of Cx26/Cx32 gap junction channel on the antineoplastic effect of etoposide in Hela cervical cancer cells.
  
  
  METHODS
  Fluorescence trace was used to assay the gap junction intercellular communication mediated by Cx26/Cx32 in Hela cells and its functional modulation by the pharmacological agents (oleamide, retinoid acid). A standard colony-forming assay was applied to determine the cell growth-inhibiting effect of etoposide in Hela cells with functional modulation of the gap junction. Hoechst 33258 staining was used to assess the changes in etoposide-induced apoptosis of Hela cells with altered gap junction functions.
  
  
  RESULTS
  Oleamide markedly decreased while retinoid acid obviously increased the gap junction function in Hela cells. Standard colony-forming assay showed that etoposide produced a lowered antiproliferative effect in Hela cells with reduced gap junction and an increased antiproliferative effect in cells with enhanced gap junction function. In cells with a reduced gap junction function, etoposide induced a lowered apoptosis rate, which increased obviously in cells with an enhanced gap junction function.
  
  
  CONCLUSIONS
  The antineoplastic effect of etoposide is reduced in Hela cells with a decreased gap junction intercellular communication mediated by Cx26/Cx32 and is enhanced in cells with an increased gap junction intercellular communication.
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, Bengbu Medical College, Bengbu, China. bbmctxh@126.com
authors.forename: Xu-Hui
authors.initials: XH
authors.lastname: Tong
authors.forename: Shu-Ying
authors.initials: SY
authors.lastname: Dong
authors.forename: Guo-Jun
authors.initials: GJ
authors.lastname: Jiang
authors.forename: Gao-Fu
authors.initials: GF
authors.lastname: Fan
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D000072259:Connexin 26; D017630:Connexins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D005047:Etoposide / Q000494:pharmacology*; D017629:Gap Junctions / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D014162:Transfection; D000097002:Gap Junction beta-1 Protein
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D000072259:Connexin 26; D017630:Connexins; D005047:Etoposide; D000097002:Gap Junction beta-1 Protein; C000607042:GJB2 protein, human