title: Engineering genes for predictable protein expression.
abstract: The DNA sequence used to encode a polypeptide can have dramatic effects on its expression. Lack of readily available tools has until recently inhibited meaningful experimental investigation of this phenomenon. Advances in synthetic biology and the application of modern engineering approaches now provide the tools for systematic analysis of the sequence variables affecting heterologous expression of recombinant proteins. We here discuss how these new tools are being applied and how they circumvent the constraints of previous approaches, highlighting some of the surprising and promising results emerging from the developing field of gene engineering.
authors.affiliation: DNA2.0 Inc., 1140 O'Brien Drive, Menlo Park, CA 94025, USA.
authors.forename: Claes
authors.initials: C
authors.lastname: Gustafsson
authors.forename: Jeremy
authors.initials: J
authors.lastname: Minshull
authors.forename: Sridhar
authors.initials: S
authors.lastname: Govindarajan
authors.forename: Jon
authors.initials: J
authors.lastname: Ness
authors.forename: Alan
authors.initials: A
authors.lastname: Villalobos
authors.forename: Mark
authors.initials: M
authors.lastname: Welch
mesh_terms: D003062:Codon; D015723:Gene Library; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors; D006801:Humans; D016366:Open Reading Frames; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics*; D058615:Synthetic Biology
chemical_list: D003062:Codon; D011994:Recombinant Proteins