title: Biomarker research and some deduction in superficial bladder cancer cells combined with corresponding stroma.
abstract: OBJECTIVE
  To facilitate biomarker discovery and improve cancer biology research of superficial bladder transitional cell carcinoma (SBTCC).
  
  
  METHODS
  Laser capture microdissection and two-dimensional liquid chromatography tandem mass spectrometry was used to identified the proteome expression profile of purified normal urothelial cells (cancer cells)/normal stromal cells (cancer stromal cells). Based on the expression profile, biomarker discovery and the mechanisms of multi-step carcinogenesis were discussed.
  
  
  RESULTS
  775/792 proteins commonly appeared in 4 paired cancer/normal tissue. 339 differential proteins located at the well-known biological KEGG pathways and defined as potential biomarkers. 53/31 proteins specific repeat expression in cancer tissue/normal tissue and thought to play important role in tumor--stroma interactions.
  
  
  CONCLUSIONS
  Proteins origin from cancer or stromal cells have the same probability to be a biomarker. The significant altered pathways mainly include metabolic pathways, spliceosome, endocytosis, oxidative phosphorylation, etc. Most of the specific repeat expression proteins act important role in cancer biology.
authors.affiliation: Department of Urology, The Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong, China. Niuhaitao1976@gmail.com
authors.forename: Hai Tao
authors.initials: HT
authors.lastname: Niu
authors.forename: Chuan Min
authors.initials: CM
authors.lastname: Yang
authors.forename: Bo
authors.initials: B
authors.lastname: Chen
authors.forename: Qian
authors.initials: Q
authors.lastname: Dong
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D002295:Carcinoma, Transitional Cell / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D061346:Laser Capture Microdissection; D008297:Male; D008875:Middle Aged; D040901:Proteomics; D017154:Stromal Cells; D001749:Urinary Bladder Neoplasms / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor