title: The lectin receptor kinase-VI.2 is required for priming and positively regulates Arabidopsis pattern-triggered immunity.
abstract: Plant cells can be sensitized toward a subsequent pathogen attack by avirulent pathogens or by chemicals such as β-aminobutyric acid (BABA). This process is called priming. Using a reverse genetic approach in Arabidopsis thaliana, we demonstrate that the BABA-responsive L-type lectin receptor kinase-VI.2 (LecRK-VI.2) contributes to disease resistance against the hemibiotrophic Pseudomonas syringae and the necrotrophic Pectobacterium carotovorum bacteria. Accordingly, LecRK-VI.2 mRNA levels increased after bacterial inoculation or treatments with microbe-associated molecular patterns (MAMPs). We also show that LecRK-VI.2 is required for full activation of pattern-triggered immunity (PTI); notably, lecrk-VI.2-1 mutants show reduced upregulation of PTI marker genes, impaired callose deposition, and defective stomatal closure. Overexpression studies combined with genome-wide microarray analyses indicate that LecRK-VI.2 positively regulates the PTI response. LecRK-VI.2 is demonstrated to act upstream of mitogen-activated protein kinase signaling, but independently of reactive oxygen production and Botrytis-induced kinase1 phosphorylation. In addition, complex formation between the MAMP receptor flagellin sensing2 and its signaling partner brassinosteroid insensitive1-associated kinase1 is observed in flg22-treated lecrk-VI.2-1 mutants. LecRK-VI.2 is also required for full BABA-induced resistance and priming of PTI. Our work identifies LecRK-VI.2 as a novel mediator of the Arabidopsis PTI response and provides insight into molecular mechanisms governing priming.
authors.affiliation: Department of Life Science and Institute of Plant Biology, National Taiwan University, Taipei 106, Taiwan.
authors.forename: Prashant
authors.initials: P
authors.lastname: Singh
authors.forename: Yi-Chun
authors.initials: YC
authors.lastname: Kuo
authors.forename: Swati
authors.initials: S
authors.lastname: Mishra
authors.forename: Chia-Hong
authors.initials: CH
authors.lastname: Tsai
authors.forename: Chih-Cheng
authors.initials: CC
authors.lastname: Chien
authors.forename: Ching-Wei
authors.initials: CW
authors.lastname: Chen
authors.forename: Marie
authors.initials: M
authors.lastname: Desclos-Theveniau
authors.forename: Po-Wei
authors.initials: PW
authors.lastname: Chu
authors.forename: Birgit
authors.initials: B
authors.lastname: Schulze
authors.forename: Delphine
authors.initials: D
authors.lastname: Chinchilla
authors.forename: Thomas
authors.initials: T
authors.lastname: Boller
authors.forename: Laurent
authors.initials: L
authors.lastname: Zimmerli
mesh_terms: D000613:Aminobutyrates / Q000494:pharmacology; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics* / Q000276:immunology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D060467:Disease Resistance; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D005816:Genetic Complementation Test; D016254:Mutagenesis, Insertional; D020411:Oligonucleotide Array Sequence Analysis; D016973:Pectobacterium carotovorum / Q000472:pathogenicity; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D057865:Plant Immunity; D054046:Plant Stomata / Q000276:immunology; D011494:Protein Kinases / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D044224:Pseudomonas syringae / Q000472:pathogenicity; D018749:RNA, Plant / Q000235:genetics
chemical_list: D000613:Aminobutyrates; D029681:Arabidopsis Proteins; D004269:DNA, Bacterial; D018749:RNA, Plant; C036483:T-DNA; C047667:3-aminobutyric acid; D011494:Protein Kinases; C463660:BAK1 protein, Arabidopsis; C411853:FLS2 protein, Arabidopsis; C509522:BIK1 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases