title: Molecular characterization of the Trypanosoma cruzi specific RNA binding protein TcRBP40 and its associated mRNAs.
abstract: Trypanosoma cruzi is the causative agent of Chagas disease, a neglected disorder that affects millions of people in the Americas. T. cruzi relies mostly upon post-transcriptional regulation to control stage specific gene expression. RNA binding proteins (RBPs) associate with functionally related mRNAs forming ribonucleoprotein complexes that define post-transcriptional operons. The RNA Recognition Motif (RRM) is the most common and ancient family of RBPs. This family of RBPs has been identified in trypanosomatid parasites and only a few of them have been functionally characterized. We describe here the functional characterization of TcRBP40, a T. cruzi specific RBP, and its associated mRNAs. We used a modified version of the recombinant RIP-Chip assay to identify the mRNAs with which it associates and in vivo TAP-tag assays to confirm these results. TcRBP40 binds to an AG-rich sequence in the 3'UTR of the associated mRNAs, which were found to encode mainly putative transmembrane proteins. TcRBP40 is differentially expressed in metacyclogenesis. Surprisingly, in epimastigotes, it is dispersed in the cytoplasm but is concentrated in the reservosomes, a T. cruzi specific organelle, which suggests a putative new function for this parasite organelle.
authors.affiliation: Instituto Carlos Chagas, Fiocruz-Paraná, Rua Professor Algacyr Munhoz Mader 3775, 81350-010 CIC Curitiba, Brazil.
authors.forename: Eloise P
authors.initials: EP
authors.lastname: Guerra-Slompo
authors.forename: Christian M
authors.initials: CM
authors.lastname: Probst
authors.forename: Daniela P
authors.initials: DP
authors.lastname: Pavoni
authors.forename: Samuel
authors.initials: S
authors.lastname: Goldenberg
authors.forename: Marco A
authors.initials: MA
authors.lastname: Krieger
authors.forename: Bruno
authors.initials: B
authors.lastname: Dallagiovanna
mesh_terms: D001483:Base Sequence; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014349:Trypanosoma cruzi / Q000378:metabolism*
chemical_list: D015800:Protozoan Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins