title: Comparison of the f-HPV typing™ and Hybrid Capture II® assays for detection of high-risk HPV genotypes in cervical samples.
abstract: Human papillomavirus genotyping is being considered in cervical screening programs and for monitoring the effectiveness of HPV vaccination. Both approaches require access to fast, easy and high-throughput technology. The aim of this study was to compare a new commercial assay (f-HPV typing™) with the Hybrid Capture II® (HC2) to detect HPV infection. The F-HPV typing is a multiplex fluorescent PCR method recognizing E6 and E7 regions of 13 high-risk (HR) HPV types, the same set of HR-types targeted HC2 test. A subset of 157 cervical samples was tested with both assays. The percentage of positive HR-HPV DNA samples was 24% (37/155) by HC2 and 33% (49/155) by f-HPV typing. Concordant results were found in 133/155 (overall agreement, 85.8%; Cohen's kappa=0.65). The analytical sensitivity and specificity of f-HPV were 97.6 and 93, respectively. In conclusion, this study shows that the f-HPV assay provides a good alternative to HC2 to detect HPV infection, allowing simple and rapid HPV genotyping and detecting multiple infections.
authors.affiliation: Retrovirology Laboratory-IrsiCaixa Foundation, Spain. PCC@general-lab.com
authors.forename: María-Paz
authors.initials: MP
authors.lastname: Cañadas
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Cirigliano
authors.forename: Laila
authors.initials: L
authors.lastname: Darwich
authors.forename: Guillermo
authors.initials: G
authors.lastname: Sirera
authors.forename: Josep
authors.initials: J
authors.lastname: Coll
authors.forename: Bonaventura
authors.initials: B
authors.lastname: Clotet
authors.forename: Sebastian
authors.initials: S
authors.lastname: Videla
mesh_terms: D002584:Cervix Uteri / Q000821:virology; D019411:Clinical Laboratory Techniques / Q000379:methods*; D005260:Female; D005838:Genotype; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000821:virology*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods*
chemical_list: D011933:Reagent Kits, Diagnostic