title: Increased incidence of DNA amplification in follicular than in uterine and blood samples indicates possible tropism of Neospora caninum to the ovarian follicle.
abstract: This study evaluated the presence of Neospora caninum in ovarian follicle aspirates and uterine flushes obtained from N. caninum seropositive dairy cows. Ninety-two cows that aborted within the previous 90 days were sampled to determine the presence of antibodies against N. caninum. Thirteen seropositive cows were chosen for collection of blood leukocytes, uterine flushes (UF; n=12) and follicular aspirates (OPU; n=13). Samples were centrifuged and the cellular sediment from the follicular fluid, uterine flushes and blood leukocytes were used for DNA extraction and PCR. Follicular aspirates had the highest frequency of DNA amplification for N. caninum (p<0.05, 92.3%; 12/13). Whereas uterine (4/12) and blood leukocyte (5/13) samples had similar (p>0.05) rate of positive results. Nonetheless, there was no agreement between blood leukocytes and follicular samples taken from the same animal (Cohen Kappa=-0.16). Similarly, blood leukocytes and uterine results had moderate agreement between them (Cohen Kappa=0.47). This study indicates that N. caninum is present in the ovarian follicle and uterus of seropositive cows, suggesting a possible risk of neosporosis transmission between females during oocyte and embryo collection and transfer. Hence, precautions should be taken to minimize the risk of N. caninum transmission. Furthermore, the high incidence of positive results in follicle samples, that exceeded that of their paired blood leukocytes, suggests a possible tropism of N. caninum for the ovarian follicle.
authors.affiliation: Universidad Nacional Autónoma de México, Facultad de Medicina Veterinaria y Zootecnia, Ciudad Universitaria, México 04510, Mexico.
authors.forename: Andressa F
authors.initials: AF
authors.lastname: Silva
authors.forename: Lucia
authors.initials: L
authors.lastname: Rangel
authors.forename: Carlos Garcia
authors.initials: CG
authors.lastname: Ortiz
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Morales
authors.forename: Eraldo L
authors.initials: EL
authors.lastname: Zanella
authors.forename: Uziel
authors.initials: U
authors.lastname: Castillo-Velázquez
authors.forename: Carlos G
authors.initials: CG
authors.lastname: Gutierrez
mesh_terms: D000034:Abortion, Veterinary; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000469:parasitology*; D003048:Coccidiosis / Q000097:blood / Q000469:parasitology / Q000662:veterinary*; D016054:DNA, Protozoan / Q000032:analysis*; D003612:Dairying; D005260:Female; D018688:Neospora / Q000502:physiology*; D006080:Ovarian Follicle / Q000469:parasitology*; D011247:Pregnancy; D014599:Uterus / Q000469:parasitology*
chemical_list: D016054:DNA, Protozoan