title: Effect of hyaluronidase immobilized using electron-beam synthesis nanotechnology on sensitivity of progenitor cells to regulatory factors.
abstract: In vitro experiments demonstrated increased colony-forming capacity of erythroid, granulomonocytic, and mesenchymal progenitors of the bone marrow and parenchymal progenitor elements of the liver after treatment with immobilized hyaluronidase. Increased sensitivity of these progenitor cells to erythropoietin, granulocyte colony-stimulating factor, fibroblast growth factor, and stem cell factor, respectively, was demonstrated. Immobilized hyaluronidase enhanced the formation of tissue-specific hepatic CFU against the background of reduced yield of stromal precursors in liver tissue culture containing insulin.
authors.affiliation: Institute of Pharmacology of Russian Academy of Medical Sciences, Siberian branch, Tomsk, Russia. mmu@pharm.tsu.ru
authors.forename: A M
authors.initials: AM
authors.lastname: Dygai
authors.forename: G N
authors.initials: GN
authors.lastname: Zyuz'kov
authors.forename: V V
authors.initials: VV
authors.lastname: Zhdanov
authors.forename: E V
authors.initials: EV
authors.lastname: Udut
authors.forename: L A
authors.initials: LA
authors.lastname: Miroshnichenko
authors.forename: T Yu
authors.initials: TY
authors.lastname: Khrichkova
authors.forename: E V
authors.initials: EV
authors.lastname: Simanina
authors.forename: L A
authors.initials: LA
authors.lastname: Stavrova
authors.forename: A V
authors.initials: AV
authors.lastname: Chaikovskiy
authors.forename: T S
authors.initials: TS
authors.lastname: Markova
authors.forename: M Yu
authors.initials: MY
authors.lastname: Minakova
authors.forename: V E
authors.initials: VE
authors.lastname: Gol'dberg
authors.forename: A V
authors.initials: AV
authors.lastname: Artamonov
authors.forename: A A
authors.initials: AA
authors.lastname: Bekarev
authors.forename: P G
authors.initials: PG
authors.lastname: Madonov
authors.forename: D N
authors.initials: DN
authors.lastname: Kinsht
authors.forename: R V
authors.initials: RV
authors.lastname: Gurto
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects; D003114:Colony-Forming Units Assay; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000494:pharmacology*; D004921:Erythropoietin / Q000494:pharmacology; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects*; D006821:Hyaluronoglucosaminidase / Q000737:chemistry / Q000494:pharmacology*; D007328:Insulin / Q000378:metabolism / Q000494:pharmacology; D008099:Liver / Q000166:cytology / Q000187:drug effects; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects*; D051379:Mice; D008808:Mice, Inbred CBA; D036103:Nanotechnology; D019089:Stem Cell Factor / Q000494:pharmacology; D046509:Tissue Culture Techniques
chemical_list: D004800:Enzymes, Immobilized; D007328:Insulin; D019089:Stem Cell Factor; D004921:Erythropoietin; D016179:Granulocyte Colony-Stimulating Factor; D005346:Fibroblast Growth Factors; D006821:Hyaluronoglucosaminidase