title: Real-time PCR detection of Mycoplasma felis in domestic cats suffering from chronic conjunctivitis (Poland).
abstract: Real-time PCR directed to intergenic spacer (IGS) noncoding region between 16S and 23S rRNA genes was used for species specific detection of Mycoplasma felis in conjunctival scrapings. Samples were collected from 57 cats suffering from chronic conjunctivitis in 2008-2010 (Wrocław, Poland). Samples from 36 cats (63.2%) were shown to be positive for Mycoplasma felis. Our research gives a first insight in the occurrence of Mycoplasma felis among domestic cats in Poland suggesting that this pathogen may constitute an underestimated cause of chronic conjunctivitis.
authors.affiliation: Department of Epizootiology with Clinic for Birds and Exotic Animals, Faculty of Veterinary Medicine, Wrocław University of Environment and Life Sciences, Norwida 31, Wrocław, Poland. ploneczka@poczta.onet.pl
authors.forename: K
authors.initials: K
authors.lastname: Płoneczka-Janeczko
authors.forename: Z
authors.initials: Z
authors.lastname: Kiełbowicz
authors.forename: J
authors.initials: J
authors.lastname: Bania
authors.forename: K
authors.initials: K
authors.lastname: Bednarek
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000453:epidemiology / Q000382:microbiology*; D002415:Cats; D002908:Chronic Disease; D003234:Conjunctivitis, Bacterial / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D009174:Mycoplasma / Q000145:classification* / Q000302:isolation & purification; D009175:Mycoplasma Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D011044:Poland / Q000453:epidemiology; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary*