title: Quantitative analysis of perfluorinated chemicals in media for in vitro fertilization and related samples.
abstract: The actual standard in vitro fertilization (IVF) protocol recommends an overnight gamete co-incubation. All of the culture media used for human IVF are supplemented with serum or albumin. In the present study, we determined the concentrations of perfluorinated chemicals (PFCs) in IVF media (IVFM) and related samples by liquid chromatography with tandem mass spectrometry (LC/MS/MS). The results indicated that the concentrations of PFOS and PFOA in the protein source were higher than those in the IVFM samples. Compared with human plasma concentrations of PFCs, PFCs in all of the IVFM samples, such as PFBS, PFHxS and PFOA, were either not detected or present at only trace levels, even when protein source was added. LC/MS/MS could be used to determine PFCs in IVFM samples in future studies of the effects of PFC exposure on intrauterine insemination.
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Hoshi University, Tokyo, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Iwasaki
authors.forename: Emi
authors.initials: E
authors.lastname: Terayama
authors.forename: Azusa
authors.initials: A
authors.lastname: Kato
authors.forename: Rie
authors.initials: R
authors.lastname: Ito
authors.forename: Koichi
authors.initials: K
authors.lastname: Saito
authors.forename: Tsunehisa
authors.initials: T
authors.lastname: Makino
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nakazawa
mesh_terms: D001802:Blood Substitutes / Q000737:chemistry; D002853:Chromatography, Liquid; D003470:Culture Media / Q000737:chemistry*; D004785:Environmental Pollutants / Q000032:analysis* / Q000737:chemistry*; D005307:Fertilization in Vitro; D054879:Halogenation; D006801:Humans; D015203:Reproducibility of Results; D012709:Serum Albumin / Q000737:chemistry; D053719:Tandem Mass Spectrometry
chemical_list: D001802:Blood Substitutes; D003470:Culture Media; D004785:Environmental Pollutants; D012709:Serum Albumin