title: Computational and serologic analysis of novel and known viruses in species human adenovirus D in which serology and genomics do not correlate.
abstract: In November of 2007 a human adenovirus (HAdV) was isolated from a bronchoalveolar lavage (BAL) sample recovered from a biopsy of an AIDS patient who presented with fever, cough, tachycardia, and expiratory wheezes. To better understand the isolated virus, the genome was sequenced and analyzed using bioinformatic and phylogenomic analysis. The results suggest that this novel virus, which is provisionally named HAdV-D59, may have been created from multiple recombination events. Specifically, the penton, hexon, and fiber genes have high nucleotide identity to HAdV-D19C, HAdV-D25, and HAdV-D56, respectively. Serological results demonstrated that HAdV-D59 has a neutralization profile that is similar yet not identical to that of HAdV-D25. Furthermore, we observed a two-fold difference between the ability of HAdV-D15 and HAdV-D25 to be neutralized by reciprocal antiserum indicating that the two hexon proteins may be more similar in epitopic conformation than previously assumed. In contrast, hexon loops 1 and 2 of HAdV-D15 and HAdV-D25 share 79.13 and 92.56 percent nucleotide identity, respectively. These data suggest that serology and genomics do not always correlate.
authors.affiliation: School of Systems Biology, George Mason University, Manassas, Virginia, United States of America.
authors.forename: Elizabeth B
authors.initials: EB
authors.lastname: Liu
authors.forename: Debra A
authors.initials: DA
authors.lastname: Wadford
authors.forename: Jason
authors.initials: J
authors.lastname: Seto
authors.forename: Maria
authors.initials: M
authors.lastname: Vu
authors.forename: Nolan Ryan
authors.initials: NR
authors.lastname: Hudson
authors.forename: Lisa
authors.initials: L
authors.lastname: Thrasher
authors.forename: Sarah
authors.initials: S
authors.lastname: Torres
authors.forename: David W
authors.initials: DW
authors.lastname: Dyer
authors.forename: James
authors.initials: J
authors.lastname: Chodosh
authors.forename: Donald
authors.initials: D
authors.lastname: Seto
authors.forename: Morris S
authors.initials: MS
authors.lastname: Jones
mesh_terms: D015492:AIDS Serodiagnosis / Q000379:methods; D017088:AIDS-Related Opportunistic Infections / Q000821:virology*; D000260:Adenoviruses, Human / Q000145:classification* / Q000235:genetics*; D001483:Base Sequence; D001992:Bronchoalveolar Lavage Fluid / Q000821:virology; D036022:Capsid Proteins / Q000235:genetics*; D019295:Computational Biology; D016679:Genome, Viral / Q000235:genetics; D023281:Genomics / Q000379:methods; D006801:Humans; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013045:Species Specificity
chemical_list: D036022:Capsid Proteins; C018315:hexon capsid protein, Adenovirus; C043322:penton protein, adenovirus