title: Effect of bacteriophage in enterotoxigenic Escherichia coli (ETEC) infected pigs.
abstract: We evaluated effect of enterotoxigenic Escherichia coli (ETEC) specific lytic phage CJ12 in ETEC infected pigs. Phage was mixed with feed at a ratio of 1:1,000 (0.1%). One week after initially providing phage mixed feed, pigs were challenged orally with 10(11) CFU of ETEC and body weight, diarrhea score, bacterial CFU and phage PFU in the feces were measured. Pigs of phage treated groups C (10(6) PFU/g) and D (10(8) PFU/g) showed more resistance to diarrhea due to ETEC infection compared to positive control group B on the third day after the initial challenge. Moreover, during the quantitation of ETEC in feces, both groups C and D showed approximately 63.92 and 60.73% reduced ETEC compared to positive control group B. Phages were successfully isolated from feces in both groups C and D during the experiment without any adverse effects, suggesting the possibility of using CJ12 as a feed additive.
authors.affiliation: Department of Infectious Diseases, College of Veterinary Medicine, KRF Zoonotic Disease Priority Research Institute, Brain Korea 21 for Veterinary Science, Seoul National University, Seoul 151-742, Korea.
authors.forename: Seung Bin
authors.initials: SB
authors.lastname: Cha
authors.forename: An Na
authors.initials: AN
authors.lastname: Yoo
authors.forename: Won Jung
authors.initials: WJ
authors.lastname: Lee
authors.forename: Min Kyoung
authors.initials: MK
authors.lastname: Shin
authors.forename: Myung Hwan
authors.initials: MH
authors.lastname: Jung
authors.forename: Seung Won
authors.initials: SW
authors.lastname: Shin
authors.forename: Young Wook
authors.initials: YW
authors.lastname: Cho
authors.forename: Han Sang
authors.initials: HS
authors.lastname: Yoo
mesh_terms: D000821:Animal Feed; D000818:Animals; D001435:Bacteriophages / Q000502:physiology*; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary; D054307:Enterotoxigenic Escherichia coli / Q000821:virology*; D004927:Escherichia coli Infections / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D005243:Feces / Q000382:microbiology; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology* / Q000517:prevention & control