title: Expression of cysteine protease cathepsin L is increased in endometrial cancer and correlates with expression of growth regulatory genes.
abstract: Proteases contribute to tumor invasion and metastasis by degrading basement membranes and extracellular matrix (ECM). In this study, we compared gene expression levels of two proteases, cysteine protease Cathepsin L2 (CTSL2) and matrix metalloproteinase MMP11, in human endometrium and endometrial cancer. Our data demonstrate CTSL2 transcript levels to be strongly elevated in endometrial cancer, particularly in G3 tumors. Furthermore, we observed a highly significant positive correlation of CTSL2 with expression of growth regulatory genes Ki-67, cyclin B1, MYBL2, p21/WAF, and HER2 receptor tyrosine kinase. Our data suggest that CTSL2 might be involved in progression of endometrial cancer.
authors.affiliation: Department of Obstetrics and Gynecology, University Medical Center Regensburg, Regensburg, Germany.
authors.forename: Maciej
authors.initials: M
authors.lastname: Skrzypczak
authors.forename: Anette
authors.initials: A
authors.lastname: Springwald
authors.forename: Claus
authors.initials: C
authors.lastname: Lattrich
authors.forename: Julia
authors.initials: J
authors.lastname: Häring
authors.forename: Susanne
authors.initials: S
authors.lastname: Schüler
authors.forename: Olaf
authors.initials: O
authors.lastname: Ortmann
authors.forename: Oliver
authors.initials: O
authors.lastname: Treeck
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002403:Cathepsins / Q000235:genetics* / Q000502:physiology; D056744:Cyclin B1 / Q000235:genetics; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics; D003546:Cysteine Endopeptidases / Q000235:genetics* / Q000502:physiology; D016889:Endometrial Neoplasms / Q000201:enzymology* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D019394:Ki-67 Antigen / Q000235:genetics; D053507:Matrix Metalloproteinase 11 / Q000235:genetics; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis
chemical_list: C493956:CDKN1A protein, human; D056744:Cyclin B1; D050759:Cyclin-Dependent Kinase Inhibitor p21; D019394:Ki-67 Antigen; D012333:RNA, Messenger; D002403:Cathepsins; D003546:Cysteine Endopeptidases; C117627:CTSV protein, human; D053507:Matrix Metalloproteinase 11