title: Roseovarius litoreus sp. nov., isolated from seawater of southern coast of Korean peninsula.
abstract: A gram-negative, non-flagellated and ovoid- to rod-shaped bacterial strain, designated GSW-M15(T), was isolated from seawater on the southern coast of South Korea. Strain GSW-M15(T) grew optimally at 30 °C, at pH 7.0-7.5 and in the presence of 2 % (w/v) NaCl. The phylogenetic trees based on 16S rRNA gene sequences revealed that strain GSW-M15(T) belonged to the genus Roseovarius. Strain GSW-M15(T) exhibited highest 16S rRNA gene sequence similarity values (98.3 and 97.5 %) to Roseovarius                         halotolerans HJ50(T) and Roseovarius                         pacificus 81-2(T) and 92.8-96.2 % sequence similarity values to the type strains of the other Roseovarius species. Strain GSW-M15(T) contained Q-10 as the predominant ubiquinone and C(18:1)                         ω7c and 11-methyl-C(18:1)                         ω7c as the major fatty acids. The major polar lipids detected in strain GSW-M15(T) were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminolipid and two unidentified lipids. The DNA G+C content of strain GSW-M15(T) was 62.9 mol% and its mean DNA-DNA relatedness values with R. halotolerans KCTC 22224(T) and R. pacificus LMG 24575(T) were 33 and 18 %, respectively. Differential phenotypic properties of strain GSW-M15(T), together with the phylogenetic and genetic distinctiveness, demonstrated that this strain is distinguishable from other Roseovarius species. On the basis of the data presented here, strain GSW-M15(T) (=KCTC 23897(T) = CCUG 62218(T)) represents a novel species of the genus Roseovarius, for which the name Roseovarius litoreus sp. nov. is proposed.
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology, 115, Yuseong, Daejeon, South Korea.
authors.forename: Yong-Taek
authors.initials: YT
authors.lastname: Jung
authors.forename: Sooyeon
authors.initials: S
authors.lastname: Park
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Yoon
mesh_terms: D001482:Base Composition; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature; D014451:Ubiquinone / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; D012965:Sodium Chloride