title: Sulfated hyaluronan and chondroitin sulfate derivatives interact differently with human transforming growth factor-β1 (TGF-β1).
abstract: This study demonstrates that the modification of hyaluronan (hyaluronic acid; Hya) and chondroitin sulfate (CS) with sulfate groups leads to different binding affinities for recombinant human transforming growth factor-β1 (TGF-β1) for comparable average degrees of sulfation (DS). In general, Hya derivates showed higher binding strength than CS derivatives. In either case, a higher degree of sulfation leads to a stronger interaction. The high-sulfated hyaluronan sHya3 (average DS≈3) exhibited the tightest interaction with TGF-β1, as determined by surface plasmon resonance and enzyme-linked immunosorbent assay. The binding strength was significantly weakened by carboxymethylation. Unmodified Hya and low-sulfated, native CS showed weak or no binding affinity. The interaction characteristics of the different sulfated glycosaminoglycans are promising for incorporation into bioengineered coatings of biomaterials to modulate growth factor binding in medical applications.
authors.affiliation: Institute of Materials Science, Max Bergmann Center of Biomaterials, Technische Universität Dresden, Dresden, Germany. Vera.Hintze@tu-dresden.de
authors.forename: V
authors.initials: V
authors.lastname: Hintze
authors.forename: A
authors.initials: A
authors.lastname: Miron
authors.forename: S
authors.initials: S
authors.lastname: Moeller
authors.forename: M
authors.initials: M
authors.lastname: Schnabelrauch
authors.forename: H-P
authors.initials: HP
authors.lastname: Wiesmann
authors.forename: H
authors.initials: H
authors.lastname: Worch
authors.forename: D
authors.initials: D
authors.lastname: Scharnweber
mesh_terms: D002809:Chondroitin Sulfates / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D006820:Hyaluronic Acid / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D017550:Spectroscopy, Fourier Transform Infrared; D013431:Sulfates / Q000737:chemistry; D020349:Surface Plasmon Resonance; D053773:Transforming Growth Factor beta1 / Q000737:chemistry*
chemical_list: D013431:Sulfates; D053773:Transforming Growth Factor beta1; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates