title: Dynamics of elimination of plasmids and expression of VEGF121 gene transfected into human mesenchymal stem cells by different methods.
abstract: We compared two methods of transfection (lipofection and electroporation) with plasmid containing VEGF121 gene in four cultures of mesenchymal stem cells from the human adipose tissue. The efficacy of transfection after 1 day, the dynamics of plasmid elimination after 3, 6, 9 days, and expression of the target gene were evaluated. Transfection by both methods failed in one of 4 cultures. Analysis of the plasmid elimination dynamics showed that the content of plasmids introduced by both methods decreased by 30-69% in all cultures by day 3 and then remained unchanged from day 3 to day 9. The expression of the target gene did not correlate with the content of plasmids in cells and varied by 2-10 times in control cells and cells transfected by both methods. Fluctuation of VEGF121 expression was not related to methylation.
authors.affiliation: Medical Genetics Research Center, Russian Academy of Medical Sciences, Moscow, Russia. smirnikhinas@gmail.com
authors.forename: S A
authors.initials: SA
authors.lastname: Smirnikhina
authors.forename: A V
authors.initials: AV
authors.lastname: Lavrov
authors.forename: N P
authors.initials: NP
authors.lastname: Bochkov
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism; D002478:Cells, Cultured; D018274:Electroporation; D015870:Gene Expression; D006801:Humans; D008055:Lipids / Q000737:chemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics*; D014162:Transfection / Q000379:methods*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics* / Q000378:metabolism
chemical_list: D008055:Lipids; C086724:Lipofectamine; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A