title: Microbacterium neimengense sp. nov., isolated from the rhizosphere of maize.
abstract: A Gram-positive, non-motile, non-spore-forming, rod-shaped bacterium, strain 7087(T), was isolated from rhizosphere of maize in China. The strain grew at 4-50 °C and at pH 4-10, with optima of 37 °C and pH 7.0, respectively. Phylogenetic analysis based on the full-length 16S rRNA gene sequence revealed that strain 7087(T) was a member of the genus Microbacterium. High levels of 16S rRNA gene similarities were found between strain 7087(T) and Microbacterium binotii DSM 19164(T) (99.8 %). However, the DNA-DNA hybridization value between strain 7087(T) and Microbacterium binotii DSM 19164(T) was 24.2 %. The DNA G+C content of strain 7087(T) was 69.9 mol%. The major fatty acids were anteiso-C(17 : 0) (36.45 %), anteiso-C(15 : 0) (36.08 %) and iso-C(16 : 0) (16.11 %). The predominant menaquinones were MK-10 (28.1 %), MK-11 (54.8 %) and MK-12 (17.1 %). The diagnostic diamino acid in the cell-wall peptidoglycan was ornithine. The major polar lipids are diphosphatidylglycerol, unknown phospholipids, an unknown glycolipid and unknown amino lipids. On the basis of these results, strain 7087(T) is considered to represent a novel species of the genus Microbacterium, for which the name Microbacterium neimengense sp. nov. is proposed. The type strain is 7087(T) ( = ACCC 03008(T) = DSM 24985(T)).
authors.affiliation: Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences/Ministry of Agriculture Key Laboratory of Crop Nutrition and Fertilization, Beijing 100081, PR China.
authors.forename: Miao
authors.initials: M
authors.lastname: Gao
authors.affiliation: Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.affiliation: Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences/Ministry of Agriculture Key Laboratory of Crop Nutrition and Fertilization, Beijing 100081, PR China.
authors.forename: Yan-Chun
authors.initials: YC
authors.lastname: Zhang
authors.affiliation: Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, PR China.
authors.forename: Xiao-Ling
authors.initials: XL
authors.lastname: Zou
authors.affiliation: Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences/Ministry of Agriculture Key Laboratory of Crop Nutrition and Fertilization, Beijing 100081, PR China.
authors.forename: Lin-Qi
authors.initials: LQ
authors.lastname: Xie
authors.affiliation: Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences/Ministry of Agriculture Key Laboratory of Crop Nutrition and Fertilization, Beijing 100081, PR China.
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Hu
authors.affiliation: Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences/Ministry of Agriculture Key Laboratory of Crop Nutrition and Fertilization, Beijing 100081, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.affiliation: Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, PR China.
authors.forename: Jun-Lian
authors.initials: JL
authors.lastname: Gao
authors.affiliation: Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences/Ministry of Agriculture Key Laboratory of Crop Nutrition and Fertilization, Beijing 100081, PR China.
authors.forename: Jian-Guang
authors.initials: JG
authors.lastname: Sun
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000032:analysis; D003313:Zea mays / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2