title: Trajectories and nuclear arrangement of PML bodies are influenced by A-type lamin deficiency.
abstract: BACKGROUND
  Promyelocytic leukaemia (PML) bodies are specific nuclear structures with functional significance for acute promyelocytic leukaemia. In this study, we analysed the trajectories of PML bodies using single-particle tracking.
  
  
  RESULTS
  We observed that the recovery of PML protein after photobleaching was ATP dependent in both wild-type (wt) and A-type lamin-deficient cells. The movement of PML bodies was faster and the nuclear area occupied by particular PML bodies was larger in A-type lamin-deficient fibroblasts compared with their wt counterparts. Moreover, dysfunction of the LMNA gene increased the frequency of mutual interactions between individual PML bodies and influenced the morphology of these domains at the ultrastructural level. As a consequence of A-type lamin deficiency, PML protein accumulated in nuclear blebs and frequently appeared at the nuclear periphery.
  
  
  CONCLUSIONS
  We suggest that the physiological function of lamin A proteins is important for events that occur in the compartment of PML bodies. This observation was confirmed in other experimental models characterised by lamin changes, including apoptosis or the differentiation of mouse embryonic stem cells.
authors.affiliation: Institute of Biophysics, Academy of Sciences of the Czech Republic, 61265 Brno, Czech Republic.
authors.forename: Lenka
authors.initials: L
authors.lastname: Stixová
authors.forename: Pavel
authors.initials: P
authors.lastname: Matula
authors.forename: Stanislav
authors.initials: S
authors.lastname: Kozubek
authors.forename: Adriána
authors.initials: A
authors.lastname: Gombitová
authors.forename: Dušan
authors.initials: D
authors.lastname: Cmarko
authors.forename: Ivan
authors.initials: I
authors.lastname: Raška
authors.forename: Eva
authors.initials: E
authors.lastname: Bártová
mesh_terms: D000818:Animals; D017209:Apoptosis; D004622:Embryo, Mammalian / Q000166:cytology; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D036681:Fluorescence Recovery After Photobleaching; D049452:Green Fluorescent Proteins / Q000378:metabolism; D045586:Intranuclear Inclusion Bodies / Q000378:metabolism* / Q000648:ultrastructure; D007700:Kinetics; D034904:Lamin Type A / Q000172:deficiency* / Q000378:metabolism; D015473:Leukemia, Promyelocytic, Acute / Q000378:metabolism*; D051379:Mice; D015203:Reproducibility of Results
chemical_list: D034904:Lamin Type A; C576142:Lmna protein, mouse; D049452:Green Fluorescent Proteins