title: Detection of influenza A virus based on fluorescence resonance energy transfer from quantum dots to carbon nanotubes.
abstract: In this paper, a simple and sensitive approach for H5N1 DNA detection was described based on the fluorescence resonance energy transfer (FRET) from quantum dots (QDs) to carbon nanotubes (CNTs) in a QDs-ssDNA/oxCNTs system, in which the QDs (CdTe) modified with ssDNA were used as donors. In the initial stage, with the strong interaction between ssDNA and oxCNTs, QDs fluorescence was effectively quenched. Upon the recognition of the target, the effective competitive bindings of it to QDs-ssDNA occurred, which decreased the interactions between the QDs-ssDNA and oxCNTs, leading to the recovery of the QDs fluorescence. The recovered fluorescence of QDs was linearly proportional to the concentration of the target in the range of 0.01-20 μM with a detection limit of 9.39 nM. Moreover, even a single-base mismatched target with the same concentration of target DNA can only recover a limited low fluorescence of QDs, illustrating the good anti-interference performance of this QDs-ssDNA/oxCNTs system. This FRET platform in the QDs-ssDNA/oxCNTs system was facilitated to the simple, sensitive and quantitative detection of virus nucleic acids and could have a wide range of applications in molecular diagnosis.
authors.affiliation: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education, China), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China.
authors.forename: Junping
authors.initials: J
authors.lastname: Tian
authors.forename: Huimin
authors.initials: H
authors.lastname: Zhao
authors.forename: Meng
authors.initials: M
authors.lastname: Liu
authors.forename: Yaqiong
authors.initials: Y
authors.lastname: Chen
authors.forename: Xie
authors.initials: X
authors.lastname: Quan
mesh_terms: D004277:DNA, Single-Stranded / Q000737:chemistry / Q000378:metabolism; D004279:DNA, Viral / Q000032:analysis; D031541:Fluorescence Resonance Energy Transfer; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics / Q000302:isolation & purification*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D010084:Oxidation-Reduction; D045663:Quantum Dots
chemical_list: D004277:DNA, Single-Stranded; D004279:DNA, Viral; D037742:Nanotubes, Carbon