title: New immunosuppressive cyclomyrsinol diterpenes from Euphorbia kopetdaghi Prokh.
abstract: Aceton: chloroform (1:2) extracts of the aerial parts of Euphorbia kopetdaghi Prokh. (Euphorbiaceae) were investigated for its diterpenoids and afforded three new five-membered ring, pentacyclic myrisinane polyester comprised of 3,5,10-O-triacetyl-8-O-isobutanoyl-14-O-benzoylcyclomyrsinol (1), 3,5,10,14-O-tetraacetyl-8-O-(2'-methyl butanoyl)-cyclomyrsinol (2) and 3,5,10,14-O-tetracetyl-8-O-isobutanoylcyclomyrsinol (3). The structures were elucidated based on (13)C- and (1)H-NMR as well as 2D-NMR, IR and different MS spectra and the immunomodulation activity for compound 1 was evaluated through lymphocyte proliferation assay, IL-2 assay, oxidative burst of phagocytic leukocytes and through their cytotoxicity on two cell lines. Compound 1 showed significant suppressive activity against phytohemagglutinin-activated T-cell proliferation with an IC(50) of 1.83 µg mL(-1), IL-2 suppressive activity with an IC(50) of 19.0 µg mL(-1) and oxidative burst suppressive activity with an IC(50) of 1.6 µg mL(-1) and ignorable cytotoxic effect on the CC-1 rat hepatocyte and 3T3-L1 mouse fibroblast cell-lines.
authors.affiliation: Isfahan Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, IR Iran. ghanadian@pharm.mui.ac.ir
authors.forename: Syed Mustafa
authors.initials: SM
authors.lastname: Ghanadian
authors.forename: Abdul Majid
authors.initials: AM
authors.lastname: Ayatollahi
authors.forename: M Ahmed
authors.initials: MA
authors.lastname: Mesaik
authors.forename: Omer Mohamed
authors.initials: OM
authors.lastname: Abdalla
mesh_terms: D041721:3T3-L1 Cells; D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D004224:Diterpenes / Q000737:chemistry* / Q000494:pharmacology*; D028481:Euphorbia / Q000737:chemistry*; D007166:Immunosuppressive Agents / Q000737:chemistry* / Q000494:pharmacology*; D008214:Lymphocytes / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D015394:Molecular Structure; D010586:Phagocytes / Q000187:drug effects; D051381:Rats
chemical_list: D004224:Diterpenes; D007166:Immunosuppressive Agents