title: The concept of in vivo airway tissue engineering.
abstract: We investigated whether decellularized pig tracheas could regenerate in vivo, without being recellularized before transplantation, using the own body as bioreactor. Decellularized pig tracheal scaffolds were intraoperative conditioned with mononuclear cells and growth and differentiation factors. During the postoperative period, the in situ regeneration was boosted by administering bioactive molecules to promote peripheral mobilization and differentiation of stem/progenitor cells and ultimately the regenerative process. Results revealed, after 2 weeks, a nearly normal trachea, with respiratory epithelium and a double-banded cartilage but without any mechanical differences compared to the native tissue. The growth factor administration resulted in a mobilization of progenitor and stem cells into the peripheral circulation and in an up-regulation of anti-apoptotic genes. Isolated stem/progenitor cells could be differentiated in vitro into several cell types, proving their multipotency. We provide evidence that the own body can be used as bioreactor to promote in vivo tissue engineering replacement. Moreover, we demonstrated the beneficial effect of additional pharmaceutical intervention for an improved engraftment of the transplant.
authors.affiliation: ACTREM, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Philipp
authors.initials: P
authors.lastname: Jungebluth
authors.forename: Augustinus
authors.initials: A
authors.lastname: Bader
authors.forename: Silvia
authors.initials: S
authors.lastname: Baiguera
authors.forename: Susanne
authors.initials: S
authors.lastname: Möller
authors.forename: Massimo
authors.initials: M
authors.lastname: Jaus
authors.forename: Mei Ling
authors.initials: ML
authors.lastname: Lim
authors.forename: Kaj
authors.initials: K
authors.lastname: Fried
authors.forename: Kristín Rós
authors.initials: KR
authors.lastname: Kjartansdóttir
authors.forename: Tetsuhiko
authors.initials: T
authors.lastname: Go
authors.forename: Heike
authors.initials: H
authors.lastname: Nave
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Harringer
authors.forename: Vanessa
authors.initials: V
authors.lastname: Lundin
authors.forename: Ana I
authors.initials: AI
authors.lastname: Teixeira
authors.forename: Paolo
authors.initials: P
authors.lastname: Macchiarini
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D002469:Cell Separation; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D006801:Humans; D007249:Inflammation / Q000473:pathology; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034421:Sus scrofa; D023822:Tissue Engineering / Q000379:methods*; D014132:Trachea / Q000473:pathology / Q000502:physiology*
chemical_list: D012333:RNA, Messenger