title: Stabilizing effect of saccharides on bovine plasma protein: a calorimetric study.
abstract: Bovine plasma proteins provide the needed amino acids for the growth and development of an organism. With the purpose of preserving the native structure, related with the protein functional properties, the oligosaccharide inulin was used as protective agent and was compared with glucose and sucrose, during freeze-drying. In the present study, the thermal stability of protein was investigated as a function of type of saccharide in a concentration range of 5-15% (w/v), and at different pHs. The effect of these variables on phase transition, thermal stability and miscibility was assessed by differential scanning calorimetry (DSC) and scanning electron microscopy (SEM). The results of thermal protein properties (denaturation temperature and enthalpy), demonstrated that endothermic transition shifted to higher temperatures, being the stabilizing effect: inulin>glucose>sucrose. The thermal behavior suggests compatibility or interactions between the components of blends. In this way, the micrographs showed a homogeneous distribution of the different phases, corroborating the miscibility in the matrix. The unfolding process was irreversible and could be adequately described by a two-state model.
authors.affiliation: Instituto de Investigación en Tecnología Química-CONICET, Facultad de Química, Bioquímica y Farmacia, UNSL, Chacabuco 915-5700 San Luis, Argentina.
authors.forename: Laura T
authors.initials: LT
authors.lastname: Rodriguez Furlán
authors.forename: Javier
authors.initials: J
authors.lastname: Lecot
authors.forename: Antonio
authors.initials: A
authors.lastname: Pérez Padilla
authors.forename: Mercedes E
authors.initials: ME
authors.lastname: Campderrós
authors.forename: Noemi
authors.initials: N
authors.lastname: Zaritzky
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000737:chemistry*; D002152:Calorimetry, Differential Scanning / Q000379:methods; D002417:Cattle; D004044:Dietary Proteins; D005519:Food Preservation / Q000379:methods*; D005612:Freeze Drying / Q000379:methods; D055951:Functional Food; D005947:Glucose / Q000737:chemistry*; D007444:Inulin / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D044367:Phase Transition; D011489:Protein Denaturation / Q000187:drug effects*; D013395:Sucrose / Q000737:chemistry*; D013696:Temperature; D013816:Thermodynamics
chemical_list: D001798:Blood Proteins; D004044:Dietary Proteins; D013395:Sucrose; D007444:Inulin; D005947:Glucose