title: Dynamic change of Numbl expression after sciatic nerve crush and its role in Schwann cell differentiation.
abstract: Numbl, as a conserved homolog of Drosophila Numb, has been implicated in early development of the nervous system, but its expression and roles in nervous system lesion and repair remained unknown. Here, we performed an acute sciatic nerve injury model in adult rats and studied the dynamic changes of Numbl expression in the sciatic nerve. Temporally, Numbl expression was sharply decreased after sciatic nerve crush and reached a valley at day 7. Spatially, Numbl was widely expressed in the normal sciatic nerve, including axons and Schwann cells, whereas, after injury, Numbl expression was decreased predominantly in Schwann cells. In vitro, we induced Schwann cell differentiation with cAMP and found that Numbl expression was decreased in the differentiated process. Depletion of Numbl could promote Schwann cell differentiation. In addition, we demonstrated that in vitro myelination was suppressed by overexpression of Numbl in Schwann cells. Collectively, we hypothesized peripheral nerve injury induced a downregulation of Numbl in the sciatic nerve, which was associated with Schwann cell differentiation.
authors.affiliation: Department of Osteology, Affiliated Jiangyin Hospital, Nantong University, Wuxi, People's Republic of China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhu
authors.forename: Yaohua
authors.initials: Y
authors.lastname: Yan
authors.forename: Kaifu
authors.initials: K
authors.lastname: Ke
authors.forename: Xinming
authors.initials: X
authors.lastname: Wu
authors.forename: Yilu
authors.initials: Y
authors.lastname: Gao
authors.forename: Aiguo
authors.initials: A
authors.lastname: Shen
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Lihua
authors.initials: L
authors.lastname: Kang
authors.forename: Guowei
authors.initials: G
authors.lastname: Zhang
authors.forename: Qiyun
authors.initials: Q
authors.lastname: Wu
authors.forename: Huiguang
authors.initials: H
authors.lastname: Yang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation / Q000502:physiology*; D015536:Down-Regulation; D005455:Fluorescent Antibody Technique; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D008297:Male; D009409:Nerve Crush; D059348:Peripheral Nerve Injuries / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D012583:Schwann Cells / Q000166:cytology* / Q000378:metabolism*; D012584:Sciatic Nerve / Q000293:injuries / Q000378:metabolism; D014162:Transfection
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; C506057:Numbl protein, rat