title: Evaluation of N-acetilglucosaminidase and myeloperoxidase activity in patients with endometriosis-related infertility undergoing intracytoplasmic sperm injection.
abstract: OBJECTIVE
  Inflammation is as an important factor in ovulation with the active participation of leucocytes and their inflammatory mediators. The present study was performed to compare the activity of the inflammatory enzymes myeloperoxidase (MPO) and N-acetylglucosaminidase (NAG) in patients with endometriosis-related infertility and in normally ovulating women undergoing intracytoplasmic sperm injection (ICSI).
  
  
  METHODS
  This prospective study included infertile women undergoing ICSI treatment. These women were divided into two groups: endometriosis anovulation (n = 18) and normally ovulating (n = 20). NAG and MPO activity was evaluated colorimetrically in serum and in follicular fluids obtained at the time of oocyte retrieval.
  
  
  RESULTS
  There was a significant correlation between the serum and follicular fluid activities of NAG and MPO (τ = 0.256, P = 0.025; and τ = -0.234, P = 0.041; respectively). Both serum and follicular fluid NAG activities were higher in patients with endometriosis compared to the control group (P < 0.001). MPO follicular fluid activity was lower in patients with endometriosis compared to normally ovulating women (P = 0.016).
  
  
  CONCLUSIONS
  Infertile patients with endometriosis show a distinct pattern of serum and follicular fluid macrophage/neutrophil activation compared to normally ovulating women undergoing ICSI, which may reflect the role of immune and inflammatory alterations in endometriosis-related infertility.
authors.affiliation: Department of Obstetrics and Gynecology, Paulista State University, Botucatu, Brazil.
authors.forename: Rívia Mara
authors.initials: RM
authors.lastname: Lamaita
authors.forename: Anaglória
authors.initials: A
authors.lastname: Pontes
authors.forename: Andrezza V
authors.initials: AV
authors.lastname: Belo
authors.forename: João Pedro
authors.initials: JP
authors.lastname: Caetano
authors.forename: Sílvia P
authors.initials: SP
authors.lastname: Andrade
authors.forename: Eduardo B
authors.initials: EB
authors.lastname: Cândido
authors.forename: Márcia Mendonça
authors.initials: MM
authors.lastname: Carneiro
authors.forename: Agnaldo L
authors.initials: AL
authors.lastname: Silva-Filho
mesh_terms: D000118:Acetylglucosaminidase / Q000378:metabolism*; D000328:Adult; D004715:Endometriosis / Q000150:complications / Q000201:enzymology*; D005260:Female; D015571:Follicular Fluid / Q000201:enzymology; D006801:Humans; D007247:Infertility, Female / Q000201:enzymology* / Q000209:etiology / Q000628:therapy; D007249:Inflammation / Q000201:enzymology; D009195:Peroxidase / Q000378:metabolism*; D011446:Prospective Studies; D020554:Sperm Injections, Intracytoplasmic
chemical_list: D009195:Peroxidase; D000118:Acetylglucosaminidase