title: Musca domestica pupae lectin induces apoptosis in HepG2 cells through a NF-κB/p65-mediated caspase pathway.
abstract: A new lectin (42 kDa) from Musca domestica pupae (MPL) has been known to inhibit proliferation in tumor cells. In this study, flow cytometry analysis showed that MPL induced HepG2 cells apoptosis significantly and the cells were arrested at S phase. MPL inhibited IκB-α degradation and NF-κB/p65 translocation from cytoplasm into nucleus. Simultaneously, the expressions of FLIP, which is a target gene of NF-κB/p65 were down-regulated and the caspase-8 and caspase-3 were then activated to induce apoptosis. Taken together, these results showed that MPL induced a caspase-dependent apoptosis via NF-κB/p65 pathway in HepG2 cells.
authors.affiliation: Ministry of Education, Tianjin University of Science and Technology, College of Food Engineering and Biotechnology, Key Laboratory of Food Safety and Sanitation, Tianjin Economy Technological Development Area, China. niejianzeng33@126.com
authors.forename: Jianzeng
authors.initials: J
authors.lastname: Nie
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Cao
authors.forename: Minghui
authors.initials: M
authors.lastname: Zhou
authors.forename: Xinyuan
authors.initials: X
authors.lastname: Zhang
authors.forename: Rongping
authors.initials: R
authors.lastname: Zhang
authors.forename: Lili
authors.initials: L
authors.lastname: Niu
authors.forename: Yan
authors.initials: Y
authors.lastname: Xia
authors.forename: Lihua
authors.initials: L
authors.lastname: Hou
authors.forename: Chunling
authors.initials: C
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D053446:CASP8 and FADD-Like Apoptosis Regulating Protein / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D053181:Caspase 8 / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D015536:Down-Regulation; D056945:Hep G2 Cells / Q000187:drug effects* / Q000502:physiology; D006793:Houseflies / Q000737:chemistry*; D006801:Humans; D037102:Lectins / Q000494:pharmacology*; D016328:NF-kappa B / Q000378:metabolism*; D011679:Pupa / Q000737:chemistry; D059807:S Phase Cell Cycle Checkpoints / Q000502:physiology; D051996:Transcription Factor RelA / Q000378:metabolism
chemical_list: D053446:CASP8 and FADD-Like Apoptosis Regulating Protein; D037102:Lectins; D016328:NF-kappa B; D051996:Transcription Factor RelA; D053148:Caspase 3; D053181:Caspase 8