title: Remotely activated protein-producing nanoparticles.
abstract: The development of responsive nanomaterials, nanoscale systems that actively respond to stimuli, is one general goal of nanotechnology. Here we develop nanoparticles that can be controllably triggered to synthesize proteins. The nanoparticles consist of lipid vesicles filled with the cellular machinery responsible for transcription and translation, including amino acids, ribosomes, and DNA caged with a photolabile protecting group. These particles served as nanofactories capable of producing proteins including green fluorescent protein (GFP) and enzymatically active luciferase. In vitro and in vivo, protein synthesis was spatially and temporally controllable, and could be initiated by irradiating micrometer-scale regions on the time scale of milliseconds. The ability to control protein synthesis inside nanomaterials may enable new strategies to facilitate the study of orthogonal proteins in a confined environment and for remotely activated drug delivery.
authors.affiliation: David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States.
authors.forename: Avi
authors.initials: A
authors.lastname: Schroeder
authors.forename: Michael S
authors.initials: MS
authors.lastname: Goldberg
authors.forename: Christian
authors.initials: C
authors.lastname: Kastrup
authors.forename: Yingxia
authors.initials: Y
authors.lastname: Wang
authors.forename: Shan
authors.initials: S
authors.lastname: Jiang
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Joseph
authors.forename: Christopher G
authors.initials: CG
authors.lastname: Levins
authors.forename: Sneha T
authors.initials: ST
authors.lastname: Kannan
authors.forename: Robert
authors.initials: R
authors.lastname: Langer
authors.forename: Daniel G
authors.initials: DG
authors.lastname: Anderson
mesh_terms: D003460:Crystallization / Q000379:methods*; D008422:Materials Testing; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D010316:Particle Size; D011487:Protein Conformation; D015202:Protein Engineering / Q000379:methods*; D011506:Proteins / Q000138:chemical synthesis*; D012371:Robotics / Q000379:methods*; D013499:Surface Properties
chemical_list: D011506:Proteins