title: Localization of residues in a novel DNA-binding domain of DmSNAP43 required for DmSNAPc DNA-binding activity.
abstract: Transcription of snRNA genes depends upon the recognition of the proximal sequence element (PSE) by the snRNA activating protein complex SNAPc. In Drosophila melanogaster, all subunits of DmSNAPc (DmSNAP43, DmSNAP50, and DmSNAP190) are required for PSE-binding activity. Previous work demonstrated that a non-canonical DmSNAP43 domain bounded by residues 193-272 was essential for DmSNAPc to bind to the PSE. In this study, the contribution of amino acid residues within this domain to DNA binding by DmSNAPc was investigated by alanine-scanning mutagenesis. The results have identified two clusters of residues within this domain required for the sequence-specific DNA-binding activity of DmSNAPc.
authors.affiliation: Department of Biology, San Diego State University, San Diego, CA 92182-1030, United States.
authors.forename: Ko-Hsuan
authors.initials: KH
authors.lastname: Hung
authors.forename: William E
authors.initials: WE
authors.lastname: Stumph
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D004247:DNA / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000502:physiology; D006801:Humans; D008969:Molecular Sequence Data; D016296:Mutagenesis; D021122:Protein Subunits / Q000235:genetics* / Q000378:metabolism*; D016415:Sequence Alignment
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; C483821:Pbp45 protein, Drosophila; D021122:Protein Subunits; D004247:DNA