title: [Molecular detection of tomato yellow leaf curl virus (TYLCV)].
abstract: Tomato yellow leaf curl virus (TYLCV) is currently considered as one of the most devastating viruses in cultivated tomatoes (Solanum lycopersicum) worldwide. We reported here the development of a PCR-based method to quickly detect TYLCV using the primer pairs (TYLCV-F: 5'-ACG CAT GCC TCT AAT CCA GTG TA-3' and TYLCV-R: 5'-CCA ATA AGG CGT AAG CGT GTA GAC-3'), which was designed based on the genome sequence of TYLCV. A TYLCV-specific band of 543 bp was amplified from infected tomato plants. This protocol provides a rapid, reliable, and sensitive tool for molecular detection and identification of TYLCV in the industrial seedling and virus resistance breeding to facilitate safe and sustainable production of tomato.
authors.affiliation: Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, China. lichangbao@nercv.org
authors.forename: Chang-Bao
authors.initials: CB
authors.lastname: Li
authors.forename: Yan-Ling
authors.initials: YL
authors.lastname: Cui
authors.forename: Li-Ying
authors.initials: LY
authors.lastname: Zhang
authors.forename: Chuan-You
authors.initials: CY
authors.lastname: Li
mesh_terms: D053839:Begomovirus / Q000235:genetics* / Q000302:isolation & purification* / Q000502:physiology; D001947:Breeding; D017931:DNA Primers / Q000235:genetics; D023281:Genomics; D016133:Polymerase Chain Reaction / Q000379:methods*; D014675:Vegetables / Q000235:genetics / Q000821:virology
chemical_list: D017931:DNA Primers