title: Nigella sativa reduces tissue damage in rat ovaries subjected to torsion and detorsion: oxidative stress, proinflammatory response and histopathological evaluation.
abstract: BACKGROUND
  To evaluate biochemically and histopathologically the effects of Nigella sativa (NS) in experimental ischemia and ischemia/reperfusion (I/R) injury in rat ovaries.
  
  
  METHODS
  Thirty-six female rats were divided into 6 groups: group I = sham operation; group II = 500 mg/kg NS + sham operation; group III = bilateral ovarian ischemia; group IV = 500 mg/kg NS + ischemia; group V = 3-hour period of ischemia + 3-hour reperfusion, and group VI: 3-hour period of ischemia + 500 mg/kg NS 2.5 h after the induction of ischemia + 3-hour reperfusion. At the end of ischemia, the bilateral vascular clips were removed, and 3-hour reperfusion was continued. IL-1β, IL-6, and TNF-α cytokine levels in serum, and superoxide dismutase (SOD), myeloperoxidase (MPO), glutathione (GSH), and malondialdehyde (MDA) levels were determined.
  
  
  RESULTS
  I/R increased the MDA level and MPO activity while significantly decreasing the SOD activity and GSH level when compared to the sham. The 500-mg/kg dose of NS before I/R reversed the trend in MDA levels, MPO activity, SOD activity, and GSH levels. Ischemia and I/R increased the serum levels of IL-1β, IL-6, and TNF-α, while the administration of NS decreased the serum levels of these cytokines.
  
  
  CONCLUSIONS
  The administration of NS is effective in reversing tissue damage induced by ischemia and/or I/R in ovaries.
authors.affiliation: Department of Biochemistry, Yenimahalle Government Hospital, Ankara, Turkey. yasinbayir@hotmail.com
authors.forename: Yasin
authors.initials: Y
authors.lastname: Bayir
authors.forename: Yalcin
authors.initials: Y
authors.lastname: Karagoz
authors.forename: Emre
authors.initials: E
authors.lastname: Karakus
authors.forename: Abdulmecit
authors.initials: A
authors.lastname: Albayrak
authors.forename: Ozlem
authors.initials: O
authors.lastname: Sengul
authors.forename: Ismail
authors.initials: I
authors.lastname: Can
authors.forename: Nuh
authors.initials: N
authors.lastname: Yayla
authors.forename: Ufuk
authors.initials: U
authors.lastname: Kuskun
authors.forename: Mevlut Sait
authors.initials: MS
authors.lastname: Keles
mesh_terms: D000818:Animals; D005260:Female; D005978:Glutathione / Q000032:analysis; D053583:Interleukin-1beta / Q000097:blood; D015850:Interleukin-6 / Q000378:metabolism; D008315:Malondialdehyde / Q000032:analysis; D031881:Nigella sativa; D009869:Oophoritis / Q000188:drug therapy* / Q000473:pathology; D010053:Ovary / Q000098:blood supply* / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects*; D009195:Peroxidase / Q000032:analysis; D008517:Phytotherapy; D028321:Plant Preparations / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000473:pathology; D013482:Superoxide Dismutase / Q000032:analysis; D054159:Torsion, Mechanical; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D053583:Interleukin-1beta; D015850:Interleukin-6; D028321:Plant Preparations; D014409:Tumor Necrosis Factor-alpha; D008315:Malondialdehyde; D009195:Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione