title: Dissection of morphological and metabolic differentiation of ameloblasts via ectopic SP6 expression.
abstract: Tooth enamel is the hardest organ in the body. In rodent incisor, the enamel is exclusively produced by ameloblasts with yellowish-brown pigmentation, indicating normal enamel formation. However, the molecular mechanisms of ameloblast differentiation and amelogenesis are not fully understood. Specificity protein (Sp) 6 has been reported as one of the critical factors for tooth development. To explore SP6 function, we generated Sp6 transgenic (Tg) rats. Unexpectedly, the enamel surfaces of the incisors in Tg rats were discolored, even though enamel formation and serum iron concentrations were normal. Histological analysis of incisors from 6-week-old Tg rats demonstrated that the ameloblast layer at the pigmentation stage was elongated up to the gingival margin with ectopic SP6 expression in longitudinal incisor sections. In contrast, the incisors from 10-week-old Tg rats revealed that the pigmented ameloblasts were morphologically changed to those of the reduced stage, concomitant with the sporadic disappearance of ectopic SP6 expression. Here we report that morphological differentiation and metabolism of the iron-containing pigment in ameloblasts are independently regulated during amelogenesis by means of ectopic SP6 expression.
authors.affiliation: Department of Molecular Biology, Institute of Health Biosciences, the University of Tokushima Graduate School, Tokushima, Japan.
authors.forename: Taro
authors.initials: T
authors.lastname: Muto
authors.forename: Keiko
authors.initials: K
authors.lastname: Miyoshi
authors.forename: Taigo
authors.initials: T
authors.lastname: Horiguchi
authors.forename: Takafumi
authors.initials: T
authors.lastname: Noma
mesh_terms: D000565:Ameloblasts / Q000166:cytology* / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D003743:Dental Enamel / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism*; D004210:Dissection; D051741:Kruppel-Like Transcription Factors / Q000235:genetics* / Q000378:metabolism; D008963:Molar / Q000166:cytology / Q000254:growth & development / Q000502:physiology*; D051381:Rats; D055647:Rats, Transgenic
chemical_list: D051741:Kruppel-Like Transcription Factors; C529065:Sp6 protein, rat