title: Fractalkine as a marker for assessment of severe acute pancreatitis.
abstract: OBJECTIVE
  The aim of this study was to study the role of fractalkine (FKN) in the development of severe acute pancreatitis (SAP) in animal model.
  
  
  METHODS
  Serum FKN levels in rat model (control, SAP6 h, 16 h, 24 h and 48 h) were determined by enzyme-linked immunosorbent assay. FKN mRNA and protein levels in the pancreas tissue were measured by reverse transcription polymerase chain reaction (RT-PCR), Western blot and immunohistochemistry.
  
  
  RESULTS
  Serum FKN level in the SAP rat increased significantly (P < 0.05 compared with the control group). FKN mRNA and protein levels in pancreas and lung increased significantly and reached the peak at 16 h after the induction of SAP, while those in kidney reached the peak at 48 h. Immunohistochemistry showed the overexpression of FKN in pancreas, lung and kidney tissue.
  
  
  CONCLUSIONS
  FKN involves in the progression of SAP and might be a valuable marker for the assessment of SAP.
authors.affiliation: Department of Gastroenterology, Ruijin Hospital, Shanghai Jiao-Tong University School of Medicine, China.
authors.forename: Li Ya
authors.initials: LY
authors.lastname: Huang
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
authors.forename: Ling Xiao
authors.initials: LX
authors.lastname: Xu
authors.forename: Yu Fen
authors.initials: YF
authors.lastname: Zhou
authors.forename: Wei Guang
authors.initials: WG
authors.lastname: Li
authors.forename: Yao Zong
authors.initials: YZ
authors.lastname: Yuan
mesh_terms: D000208:Acute Disease; D000818:Animals; D054428:Chemokine CX3CL1 / Q000235:genetics* / Q000378:metabolism*; D004195:Disease Models, Animal; D015870:Gene Expression / Q000502:physiology; D007668:Kidney / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D010179:Pancreas / Q000378:metabolism*; D010195:Pancreatitis / Q000175:diagnosis / Q000378:metabolism* / Q000401:mortality; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012720:Severity of Illness Index
chemical_list: D054428:Chemokine CX3CL1; C515540:Cx3cl1 protein, rat; D012333:RNA, Messenger