title: Metabolic adaptations to arsenic-induced oxidative stress in male wistar rats.
abstract: The present study was planned to investigate the effect of arsenic in rats on several biochemical indices of oxidative stress. Rats were exposed to arsenite in drinking water for upto 12 weeks. Chronic exposure to arsenic for a period of 12 weeks significantly (p < 0.05) increased arsenic burden in blood, liver, and kidney. Several intrinsic antioxidant defenses were activated after a 4-week exposure to arsenic. Some remained elevated, but others became depressed over a longer exposure period. Alterations in most of the biochemical variables reached statistical significant (p < 0.05). Arsenic significantly (p < 0.01) reduced mRNA expression of the superoxide dismutase 2 (SOD2) gene with respect to the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene. These observations indicated that prolong exposure to arsenic causes induction of oxidative stress and biochemical alterations.
authors.affiliation: Indian Veterinary Research Institute, Eastern Regional Station, Kolkata 700 037, India.
authors.forename: Tanmoy
authors.initials: T
authors.lastname: Rana
authors.forename: Asit Kumar
authors.initials: AK
authors.lastname: Bera
authors.forename: Subhashree
authors.initials: S
authors.lastname: Das
authors.forename: Debasis
authors.initials: D
authors.lastname: Bhattacharya
authors.forename: Diganta
authors.initials: D
authors.lastname: Pan
authors.forename: Subrata Kumar
authors.initials: SK
authors.lastname: Das
mesh_terms: D000818:Animals; D001151:Arsenic / Q000493:pharmacokinetics / Q000633:toxicity*; D001835:Body Weight / Q000187:drug effects; D002374:Catalase / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects; D007668:Kidney / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010587:Phagocytosis / Q000187:drug effects; D050050:Protein Carbonylation / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D000099156:Superoxide Dismutase 2
chemical_list: D001151:Arsenic; D002374:Catalase; D009569:Nitric Oxide; D013482:Superoxide Dismutase; D000099156:Superoxide Dismutase 2