title: Inhibition of fatty acid translocase cluster determinant 36 (CD36), stimulated by hyperglycemia, prevents glucotoxicity in INS-1 cells.
abstract: The purpose of the present study was to determine whether exposure of pancreatic islets to glucotoxic conditions changes fatty acid translocase cluster determinant 36 (CD36) and examine the role of CD36 on the induction of glucotoxicity. We measured the changes of CD36 and insulin secretion in high glucose (30 mM) exposed INS-1 cells and CD36 suppressed INS-1 cells by transfection of CD36 siRNA. The intracellular peroxide level of INS-1 cells increased in the high glucose media compared to normal glucose (5.6mM) media. The mRNA levels of insulin and PDX-1, as well as glucose stimulated insulin secretion (GSIS) were decreased in INS-1 cells exposed to high glucose media compared to normal glucose media, while CD36 and palmitate uptake were significantly elevated with exposure to high glucose media for 12h. The inhibition of CD36 reversed the decreased GSIS and intracellular peroxide level in INS-1 cells. These results suggest that high glucose may exacerbate glucotoxicity via increasing fatty acid influx by elevation of CD36 expression, and that CD36 may be a possible target molecule for preventing glucotoxicity in pancreatic beta-cells.
authors.affiliation: Department of Physiology, School of Medicine, Yeungnam University, Daegu, Republic of Korea.
authors.forename: Yong-Woon
authors.initials: YW
authors.lastname: Kim
authors.forename: Jun Sung
authors.initials: JS
authors.lastname: Moon
authors.forename: Ye Jin
authors.initials: YJ
authors.lastname: Seo
authors.forename: So-Young
authors.initials: SY
authors.lastname: Park
authors.forename: Jong-Yeon
authors.initials: JY
authors.lastname: Kim
authors.forename: Ji Sung
authors.initials: JS
authors.lastname: Yoon
authors.forename: In-Kyu
authors.initials: IK
authors.lastname: Lee
authors.forename: Hyoung Woo
authors.initials: HW
authors.lastname: Lee
authors.forename: Kyu Chang
authors.initials: KC
authors.lastname: Won
mesh_terms: D018955:CD36 Antigens / Q000378:metabolism*; D045744:Cell Line, Tumor; D005227:Fatty Acids / Q000378:metabolism; D005947:Glucose / Q000037:antagonists & inhibitors* / Q000378:metabolism / Q000633:toxicity; D006801:Humans; D006943:Hyperglycemia / Q000201:enzymology*; D050417:Insulin-Secreting Cells / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology
chemical_list: D018955:CD36 Antigens; D005227:Fatty Acids; D005947:Glucose