title: LncRNA proﬁle of glioblastoma reveals the potential role of lncRNAs             in contributing to glioblastoma pathogenesis.
abstract: Long non-coding RNAs (lncRNAs) have recently emerged as a major class of             regulatory molecules involved in a broad range of biological processes and complex             diseases. Our aim was to identify important lncRNAs that might play an important             role in contributing to glioblastoma (GBM) pathogenesis by conducting lncRNA and             mRNA proﬁle comparison between GBM and normal brain tissue. The differentially             expressed lncRNA and mRNA proﬁles of the tissue between GBM patient and age-matched             donor without GBM diseases were analyzed using microarrays. We propose a novel             model for the identification of lncRNA-mRNA targeting relationships that combine             the potential targets of the differentially expressed lncRNAs with the differentially             expressed mRNA abundance data. Bioinformatic analysis of the predicted target             genes (gene ontology, pathway and network analysis) was performed for further             research. The lncRNA microarray reveals differentially expressed lncRNAs between             GBM and normal brain tissues. In the GBM group, 654 lncRNAs were upregulated and             654 were downregulated (fold change ≥4.0 or ≤0.25, P<0.01). We found 104 matched             lncRNA-mRNA pairs for 91 differentially expressed lncRNAs and 84 differentially             expressed genes. Target gene-related pathway analysis showed signiﬁcant changes             in PPAR pathways in the GBM group compared with the normal brain group (P<0.05).             By further conducting lncRNA gene network analysis, we found that ASLNC22381 and             ASLNC2081 were likely to play roles in the regulation of glioma signaling pathways.             In conclusion, our results indicated that the lncRNA expression proﬁle in GBM             tissue was signiﬁcantly altered. These results may provide important insights             into the mechanisms responsible for GBM progression and pathogenesis. This study             also suggests that ASLNC22381 and ASLNC20819 may play important roles via their             target IGF-1 in the recurrence and malignant progression of GBM.
authors.affiliation: Department of Neurosurgery, Laboratory of Neuro-Oncology, Tianjin                     Medical University General Hospital, Tianjin 300052, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Han
authors.forename: Kailiang
authors.initials: K
authors.lastname: Zhang
authors.forename: Zhendong
authors.initials: Z
authors.lastname: Shi
authors.forename: Junxia
authors.initials: J
authors.lastname: Zhang
authors.forename: Jialin
authors.initials: J
authors.lastname: Zhu
authors.forename: Shanjun
authors.initials: S
authors.lastname: Zhu
authors.forename: Anling
authors.initials: A
authors.lastname: Zhang
authors.forename: Zhifan
authors.initials: Z
authors.lastname: Jia
authors.forename: Guangxiu
authors.initials: G
authors.lastname: Wang
authors.forename: Shizhu
authors.initials: S
authors.lastname: Yu
authors.forename: Peiyu
authors.initials: P
authors.lastname: Pu
authors.forename: Lun
authors.initials: L
authors.lastname: Dong
authors.forename: Chunsheng
authors.initials: C
authors.lastname: Kang
mesh_terms: D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D016022:Case-Control Studies; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D053263:Gene Regulatory Networks; D005909:Glioblastoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D047492:Peroxisome Proliferator-Activated Receptors / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D022661:RNA, Untranslated / Q000235:genetics* / Q000378:metabolism
chemical_list: D047492:Peroxisome Proliferator-Activated Receptors; D012333:RNA, Messenger; D022661:RNA, Untranslated