title: Post-Aire maturation of thymic medullary epithelial cells involves selective expression of keratinocyte-specific autoantigens.
abstract: The autoimmune regulator (Aire)-directed ectopic expression of tissue-specific antigens (TSAs) by mature medullary thymic epithelial cells (mTECs) has been viewed as an essential mechanism in the induction of central tolerance. Recent data suggest that the survival of mTECs extends beyond the Aire+ cell population to form the post-Aire mTEC population and Hassall's corpuscles (HCs). The nature and function of these post-Aire epithelial cells and structures, however, have remained unidentified. In this study, we characterized in detail the end-stage development of mTECs and HCs in both Aire-sufficient and Airedeficient mice. In addition, using a transgenic mouse model in which the LacZ reporter gene is under the control of the endogenous Aire promoter, we purified and analyzed the post-Aire mTECs to characterize their function. We showed that the end-stage maturation of mTECs closely resembles that of keratinocytes and that the lack of Aire results in a marked block of mTEC differentiation, which is partially overcome by ligands for RANK and CD40. We also provide evidence that, during mTEC development, Aire is expressed only once and during a limited 1-2 day period. The following loss of Aire expression is accompanied by a quick downregulation of MHC class II and CD80, and of most of the Aire-dependent and Aire-independent TSAs, with the exception of keratinocyte-specific genes. In the final stage of maturation, the mTECs lose their nuclei to become HCs and specifically express desmogleins (DGs) 1 and 3, which, via cross-presentation by APCs, may contribute to tolerance against these pemphigus vulgaris-related TSAs.
authors.affiliation: Molecular Pathology, Institute of General and Molecular Pathology, University of Tartu, Tartu, Estonia.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Wang
authors.forename: Martti
authors.initials: M
authors.lastname: Laan
authors.forename: Rudolf
authors.initials: R
authors.lastname: Bichele
authors.forename: Kai
authors.initials: K
authors.lastname: Kisand
authors.forename: Hamish S
authors.initials: HS
authors.lastname: Scott
authors.forename: Pärt
authors.initials: P
authors.lastname: Peterson