title: Evaluation of the VP22 gene adjuvant for enhancement of DNA vaccine against somatostatin in mice.
abstract: Somatostatin (SS) is a hormone that inhibits the secretion of growth hormone. Immunization against SS can promote the growth of animals. A novel SS-VP22 fused vaccine, pEGS2SS-V, was constructed from pEGS2SS plasmid with a VP22 gene fragment. Two times of immunization with pEGS2SS-V-induced anti-SS antibodies in mice. Compared with mice immunized with pEGS2SS and 0.85% saline, the growth performance of mice immunized with pEGS2SS-V was increased by 14.1% (P < 0.05) and 48.4% (P < 0.01) on the 2nd week after the first vaccination, respectively. The results indicated that the effects of the somatostatin DNA vaccine could be improved effectively by VP22 gene adjuvant.
authors.affiliation: Key lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070 PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Han
authors.forename: A X
authors.initials: AX
authors.lastname: Liang
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.forename: M
authors.initials: M
authors.lastname: Fang
authors.forename: G H
authors.initials: GH
authors.lastname: Hua
authors.forename: L
authors.initials: L
authors.lastname: Sang
authors.forename: L Y
authors.initials: LY
authors.lastname: Geng
authors.forename: H R
authors.initials: HR
authors.lastname: Wang
authors.forename: L G
authors.initials: LG
authors.lastname: Yang