title: Comparative analysis of tumor-infiltrating lymphocytes in a syngeneic mouse model of oral cancer.
abstract: OBJECTIVE
  To perform a comparative analysis of infiltrating immune cells in a newly developed C57BL/6 background syngeneic transplantable mouse oral cancer (MOC) model.
  
  
  METHODS
  Scientific study in an academic medical center.
  
  
  METHODS
  Use of carcinogen-induced tumorigenesis, tissue culture, cell line transplantation, and flow cytometric analysis techniques.
  
  
  RESULTS
  Previously, the authors established a series of cell line models that displayed dichotomous growth phenotypes when transplanted into immunocompetent mice. They now show that the indolent growth pattern of the MOC1-generated tumors is associated with increased baseline and inducible major histocompatibility complex class I expression and increased CD8(+) T-cell infiltration into the tumor microenvironment. Conversely, the aggressive and metastatic pattern of MOC2-generated tumors has decreased basal and inducible class I expression and is associated with FOXP3(+)CD4(+) regulatory T-cell infiltration. Delayed primary tumor growth after targeted monoclonal antibody therapy of these FOXP3(+) regulatory cells further suggests that these immune cells contribute to the aggressive phenotype of MOC2.
  
  
  CONCLUSIONS
  These data validate that key infiltrating immune cells identified here parallel findings in human head and neck cancer, making this newly developed syngeneic model a critical platform for the continued dissection of tumor-host interactions in head and neck cancer.
authors.affiliation: Department of Otolaryngology, Washington University School of Medicine, St Louis, Missouri 63110, USA.
authors.forename: Nancy P
authors.initials: NP
authors.lastname: Judd
authors.forename: Clint T
authors.initials: CT
authors.lastname: Allen
authors.forename: Ashley E
authors.initials: AE
authors.lastname: Winkler
authors.forename: Ravindra
authors.initials: R
authors.lastname: Uppaluri
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000473:pathology; D045744:Cell Line, Tumor; D004268:DNA-Binding Proteins / Q000235:genetics; D004195:Disease Models, Animal; D051858:Forkhead Transcription Factors / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology; D015395:Histocompatibility Antigens Class I / Q000032:analysis / Q000235:genetics; D008207:Lymphatic Metastasis / Q000235:genetics / Q000473:pathology; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009062:Mouth Neoplasms / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D010641:Phenotype; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000473:pathology; D014185:Transplantation, Isogeneic
chemical_list: D000911:Antibodies, Monoclonal; D004268:DNA-Binding Proteins; D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; D015395:Histocompatibility Antigens Class I; C499308:Rag2 protein, mouse