title: Genome-wide analysis of DNA methylation and gene expression changes in two Arabidopsis ecotypes and their reciprocal hybrids.
abstract: Heterosis is a fundamental biological phenomenon characterized by the superior performance of a hybrid over its parents in many traits, but the underlying molecular basis remains elusive. To investigate whether DNA methylation plays a role in heterosis, we compared at single-base-pair resolution the DNA methylomes of Arabidopsis thaliana Landsberg erecta and C24 parental lines and their reciprocal F1 hybrids that exhibited heterosis. Both hybrids displayed increased DNA methylation across their entire genomes, especially in transposable elements. Interestingly, increased methylation of the hybrid genomes predominantly occurred in regions that were differentially methylated in the two parents and covered by small RNAs, implying that the RNA-directed DNA methylation (RdDM) pathway may direct DNA methylation in hybrids. In addition, we found that 77 genes sensitive to methylome remodeling were transcriptionally repressed in both reciprocal hybrids, including genes involved in flavonoid biosynthesis and two circadian oscillator genes circadian clock associated1 and late elongated hypocotyl. Moreover, growth vigor of F1 hybrids was compromised by treatment with an agent that demethylates DNA and by abolishing production of functional small RNAs due to mutations in Arabidopsis RNA methyltransferase HUA enhancer1. Together, our data suggest that genome-wide remodeling of DNA methylation directed by the RdDM pathway may play a role in heterosis.
authors.affiliation: Peking-Yale Joint Center for Plant Molecular Genetics and Agro-Biotechnology, State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Huaishun
authors.initials: H
authors.lastname: Shen
authors.forename: Hang
authors.initials: H
authors.lastname: He
authors.forename: Jigang
authors.initials: J
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Xuncheng
authors.initials: X
authors.lastname: Wang
authors.forename: Lan
authors.initials: L
authors.lastname: Guo
authors.forename: Zhiyu
authors.initials: Z
authors.lastname: Peng
authors.forename: Guangming
authors.initials: G
authors.lastname: He
authors.forename: Shangwei
authors.initials: S
authors.lastname: Zhong
authors.forename: Yijun
authors.initials: Y
authors.lastname: Qi
authors.forename: William
authors.initials: W
authors.lastname: Terzaghi
authors.forename: Xing Wang
authors.initials: XW
authors.lastname: Deng
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000378:metabolism; D002678:Chimera / Q000235:genetics; D019175:DNA Methylation; D018744:DNA, Plant / Q000378:metabolism*; D060146:Ecotype; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant; D015698:Genomic Library; D006823:Hybrid Vigor; D018749:RNA, Plant / Q000235:genetics; D059467:Transcriptome
chemical_list: D029681:Arabidopsis Proteins; D018744:DNA, Plant; C450825:HEN1 protein, Arabidopsis; D018749:RNA, Plant