title: Expression of a recombinant extracellular fragment of human vascular endothelial growth factor receptor VEGFR1 in E. coli.
abstract: Human vascular endothelium growth factor receptor VEGFR1 is a type III fms-like tyrosine kinase with weakly pronounced tyrosine kinase function. The second and third IgG-like domains of the extracellular part of VEGFR1 act as "traps" for VEGF and are prospective candidates for antiangiogenic therapy of VEGF-dependent tumors. cDNA encoding extracellular Ig-like domains 2, 3, 4 of VEGFR1 was cloned in expressing vectors pET28a, pET32a, and pQE60. The recombinant protein was expressed in E. coli cells and purified by metal affinity chromatography. An expressing construction and a superproducer strain were created, allowing the production of high amounts of recombinant VEGFR1 extracellular fragment, needed for experimental in vivo antiangiogenic therapy.
authors.affiliation: Department of Medical Nanobiotechnologics, N.I. Pirogov Russian State Medical University, Russia. annavleopold@yandex.ru
authors.forename: A V
authors.initials: AV
authors.lastname: Leopold
authors.forename: V P
authors.initials: VP
authors.lastname: Baklaushev
authors.forename: K A
authors.initials: KA
authors.lastname: Pavlov
authors.forename: V P
authors.initials: VP
authors.lastname: Chekhonin
mesh_terms: D003001:Cloning, Molecular / Q000379:methods; D018076:DNA, Complementary / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D005822:Genetic Vectors; D006801:Humans; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D011994:Recombinant Proteins; D040281:Vascular Endothelial Growth Factor Receptor-1