title: Plant lipases: partial purification of Carica papaya lipase.
abstract: Lipases from plants have very interesting features for application in different fields. This chapter provides an overview on some of the most important aspects of plant lipases, such as sources, applications, physiological functions, and specificities. Lipases from laticifers and particularly Carica papaya lipase (CPL) have emerged as a versatile autoimmobilized biocatalyst. However, to get a better understanding of CPL biocatalytic properties, the isolation and purification of individual C. papaya lipolytic enzymes become necessary. In this chapter, a practical protocol for partial purification of the latex-associated lipolytic activity from C. papaya is given.
authors.affiliation: Industrial Biotechnology Unit, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C. (CIATEJ), Guadalajara, Jalisco, Mexico.
authors.forename: Ivanna
authors.initials: I
authors.lastname: Rivera
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Mateos-Díaz
authors.forename: Georgina
authors.initials: G
authors.lastname: Sandoval
mesh_terms: D055162:Biocatalysis; D029441:Carica / Q000737:chemistry*; D007840:Latex / Q000737:chemistry / Q000302:isolation & purification*; D008049:Lipase / Q000737:chemistry / Q000302:isolation & purification*; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification*; D013237:Stereoisomerism; D013379:Substrate Specificity
chemical_list: D007840:Latex; D010940:Plant Proteins; D008049:Lipase