title: Location of chromosomes in the nucleus of human mesenchymal stem cells.
abstract: For evaluation of the spatial structure of chromatin in nuclei of mesenchymal SC we determined the position of centromeres and individual chromosomes in interphase nucleus of mesenchymal SC. More than 300 nuclei in 7 cultures of mesenchymal SC were analyzed. Centromeres of chromosomes 6, 8, and 11 lie at a longer (0.68, 0.67, 0.7), while centromere of chromosome 18 at a shorter radial distance (0.49). Homologues of each chromosome had different radial distances. No differences in radial distances of centromeres were detected between mesenchymal SC from the adipose tissue and BM. After passage 8, distal displacement of chromosome 6 centromere (from 0.66 to 0.72) was observed, which probably indicates aging or spontaneous differentiation of cells.
authors.affiliation: Medical Genetic Research Center, Russian Academy of Medical Sciences, Moscow, Russia. avlavrov@yandex.ru
authors.forename: A V
authors.initials: AV
authors.lastname: Lavrov
authors.forename: Y I
authors.initials: YI
authors.lastname: Voldgorn
mesh_terms: D002467:Cell Nucleus / Q000648:ultrastructure*; D002503:Centromere; D002877:Chromosomes, Human; D005434:Flow Cytometry; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D059630:Mesenchymal Stem Cells / Q000648:ultrastructure*