title: [Anti-apoptotic role of mitochondrial aldehyde dehydrogenase 2 in myocardial ischemia/reperfusion injury in diabetic rats].
abstract: OBJECTIVE
  To evaluate the anti-apoptotic effect of aldehyde dehydrogenase 2 (ALDH2) on myocardial ischemia/reperfusion (I/R) injury in diabetic rats.
  
  
  METHODS
  Normal male SD rats were divided into normal, diabetes and ethanol (the agonist of ALDH2) + diabetes groups. In the latter two groups, diabetes was induced by an intraperitoneal injection of 55 mg/kg STZ. Four weeks after the modeling, myocardial I/R was mimicked ex vivo, and lactate dehydrogenase (LDH) content in the coronary flow was determined. The activities of caspase-3 and ALDH2 were evaluated, and the expressions of Bcl-2 and Bax mRNA in the left anterior myocardium were detected using RT-PCR.
  
  
  RESULTS
  In diabetic group, LDH release and caspase-3 activity were increased, while ALDH2 activity and Bcl-2/Bax mRNA expression were decreased as compared to those in normal control group. Compared with the diabetic group, ALDH2 agonist ethanol significantly reduced LDH release and caspase-3 activity, increased ALDH2 activity and Bcl-2/Bax mRNA expression.
  
  
  CONCLUSIONS
  In diabetic rats, enhanced ALDH2 expression can offer mycardial protection possibly in relation to suppress cell apoptosis.
authors.affiliation: Department of Cardiovascular Medicine, First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, China. docwhj1101@163.com
authors.forename: Hong-Ju
authors.initials: HJ
authors.lastname: Wang
authors.forename: Pin-Fang
authors.initials: PF
authors.lastname: Kang
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Ye
authors.forename: Ying
authors.initials: Y
authors.lastname: Yu
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Wang
authors.forename: Qin
authors.initials: Q
authors.lastname: Gao
mesh_terms: D000444:Aldehyde Dehydrogenase / Q000378:metabolism*; D000071396:Aldehyde Dehydrogenase, Mitochondrial; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000201:enzymology*; D000431:Ethanol / Q000494:pharmacology; D008297:Male; D024101:Mitochondrial Proteins / Q000819:agonists / Q000378:metabolism*; D017202:Myocardial Ischemia / Q000201:enzymology* / Q000209:etiology; D015428:Myocardial Reperfusion Injury / Q000201:enzymology / Q000473:pathology / Q000517:prevention & control*; D009206:Myocardium / Q000201:enzymology / Q000473:pathology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: C494828:Bax protein, rat; D024101:Mitochondrial Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D000431:Ethanol; D000444:Aldehyde Dehydrogenase; D000071396:Aldehyde Dehydrogenase, Mitochondrial; C505112:Aldh2 protein, rat; C505410:Casp3 protein, rat; D053148:Caspase 3