title: Vitrification-based cryopreservation of shoot-tips of Pinus kesiya Royle ex. Gord.
abstract: The present investigation was aimed at developing a protocol for long-term preservation of germplasm of Pinus kesiya Royle ex. Gord. through vitrification. Some of the critical components affecting explant tolerance to cryopreservation, such as effects of preculture, vitrification solutions, exposure time to vitrification solutions, volume of vitrification solution and its toxicity, washing of vitrified tissues after thawing, were analysed. The results showed that shoot regrowth of P. kesiya shoot-tips was considerably affected when exposed to cryoprotectants for longer periods of time (longer than 10 min). Among different vitrification solutions studied, maximum survival (76 percent) of shoot-tips was achieved with mVSL (using 0.6 ml of the solution) in MS basal medium containing 4.0 mg l-1 N6-benzyladenine (BA).
authors.affiliation: Division of Plant Biotechnology, Department of Botany, School of Life Sciences, North-Eastern Hill University, Shillong, Meghalaya, India.
authors.forename: V
authors.initials: V
authors.lastname: Kalita
authors.forename: H
authors.initials: H
authors.lastname: Choudhury
authors.forename: S
authors.initials: S
authors.lastname: Kumaria
authors.forename: P
authors.initials: P
authors.lastname: Tandon
mesh_terms: D001593:Benzyl Compounds; D003080:Cold Temperature; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents; D003470:Culture Media; D004121:Dimethyl Sulfoxide; D019855:Ethylene Glycol; D055993:Germ Cells, Plant / Q000166:cytology* / Q000502:physiology; D005990:Glycerol; D007701:Kinetin; D009994:Osmolar Concentration; D028223:Pinus / Q000166:cytology* / Q000502:physiology; D018520:Plant Shoots / Q000254:growth & development; D058439:Plant Somatic Embryogenesis Techniques; D011687:Purines; D012038:Regeneration; D013395:Sucrose; D058989:Vitrification
chemical_list: D001593:Benzyl Compounds; D003451:Cryoprotective Agents; D003470:Culture Media; D011687:Purines; D013395:Sucrose; D019855:Ethylene Glycol; C480551:benzylaminopurine; D007701:Kinetin; D005990:Glycerol; D004121:Dimethyl Sulfoxide