title: BALB/c-Fcgr2bPdcd1 mouse expressing anti-urothelial antibody is a novel model of autoimmune cystitis.
abstract: We report the impact of anti-urothelial autoantibody (AUAb) on urinary bladder phenotype in BALB/c mice deficient of the FcγRIIb and PD-1. AUAb was present in serum samples from approximately half of the double-knockout (DKO) mice, as detected by immunofluorescence and immunoblots for urothelial proteins including uroplakin IIIa. The AUAb-positive DKO mice showed degeneration of urothelial plaque and umbrella cells, along with infiltration of inflammatory cells in the suburothelial layer. TNFα and IL-1β were upregulated in the bladder and the urine of AUAb-positive DKO mice. Voiding behavior of mice was analyzed by the Voided Stain on Paper method. 10-week-old and older AUAb-positive DKO mice voided significantly less urine per void than did wild type (WT) mice. Furthermore, administration of the AUAb-containing serum to WT mice significantly reduced their urine volume per void. In summary, this report presents a novel comprehensive mouse model of autoimmune cystitis.
authors.affiliation: Department of Urology, Kyoto University, Kyoto, Japan.
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Sugino
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Nishikawa
authors.forename: Koji
authors.initials: K
authors.lastname: Yoshimura
authors.forename: Sadako
authors.initials: S
authors.lastname: Kuno
authors.forename: Yukio
authors.initials: Y
authors.lastname: Hayashi
authors.forename: Naoki
authors.initials: N
authors.lastname: Yoshimura
authors.forename: Taku
authors.initials: T
authors.lastname: Okazaki
authors.forename: Akihiro
authors.initials: A
authors.lastname: Kanematsu
authors.forename: Osamu
authors.initials: O
authors.lastname: Ogawa
mesh_terms: D000818:Animals; D001327:Autoimmune Diseases / Q000276:immunology; D015153:Blotting, Western; D003556:Cystitis / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D051379:Mice; D008807:Mice, Inbred BALB C; D061026:Programmed Cell Death 1 Receptor / Q000235:genetics*; D017452:Receptors, IgG / Q000235:genetics*; D019459:Urothelium / Q000276:immunology*
chemical_list: C499172:Fcgr2b protein, mouse; C491383:Pdcd1 protein, mouse; D061026:Programmed Cell Death 1 Receptor; D017452:Receptors, IgG