title: Inhibition of collagen deposit in obstructed rat bladder outlet by transplantation of superparamagnetic iron oxide-labeled human mesenchymal stem cells as monitored by molecular magnetic resonance imaging (MRI).
abstract: Bladder outlet obstruction (BOO) caused by collagen deposit is one of the most common problems in elderly males. The present study is to investigate if human mesenchymal stem cells (MSCs) are capable of inhibiting collagen deposition and improve cystometric parameters in bladder outlet  obstruction in rats. Human MSCs were labeled with nanoparticles containing superparamagnetic iron oxide (SPION), and transplanted in rat BOO lesion site. Forty 6-week-old female Sprague-Dawley rats were divided into four groups (group 1: control, group 2: sham operation, group 3: BOO, and  group 4: BOO rats receiving SPION-hMSCs). Two weeks after the onset of BOO, 1 × 10(6) SPION-hMSCs were injected into the bladder wall. Serial T2-weighted MR images were taken immediately after transplantation of SPION-labeled human MSCs and at 4 weeks posttransplantation. T2-weighted  MR images showed a clear hypointense signal induced by the SPION-labeled MSCs. While the expression of collagen and TGF-β protein increased after BOO, the expression of both returned to the original levels after MSC transplantation. Expression of HGF and c-met protein also increased in  the group with MSC transplantation. Maximal voiding pressure and residual urine volume increased after BOO but they recovered after MSC transplantation. Human MSCs transplanted in rat BOO models inhibited the bladder fibrosis and mediated recovery of bladder dysfunction. Transplantation of  MSC-based cell therapy could be a novel therapeutic strategy against bladder fibrosis in patients with bladder outlet obstruction.
authors.affiliation: Medical Research Institute, Chung-Ang University College of Medicine, Seoul, Korea.
authors.forename: Hong Jun
authors.initials: HJ
authors.lastname: Lee
authors.forename: Jong Ho
authors.initials: JH
authors.lastname: Won
authors.forename: Seung Hwan
authors.initials: SH
authors.lastname: Doo
authors.forename: Jung Hoon
authors.initials: JH
authors.lastname: Kim
authors.forename: Ki Young
authors.initials: KY
authors.lastname: Song
authors.forename: Sun Ju
authors.initials: SJ
authors.lastname: Lee
authors.forename: Inja
authors.initials: I
authors.lastname: Lim
authors.forename: Kyu-Tae
authors.initials: KT
authors.lastname: Chang
authors.forename: Yun Seob
authors.initials: YS
authors.lastname: Song
authors.forename: Seung U
authors.initials: SU
authors.lastname: Kim
mesh_terms: D000818:Animals; D003094:Collagen / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D005290:Ferric Compounds / Q000737:chemistry*; D017228:Hepatocyte Growth Factor / Q000378:metabolism; D006801:Humans; D008279:Magnetic Resonance Imaging; D008280:Magnetics; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D053768:Metal Nanoparticles / Q000737:chemistry; D019859:Proto-Oncogene Proteins c-met / Q000378:metabolism; D011877:Radionuclide Imaging; D051381:Rats; D017207:Rats, Sprague-Dawley; D016212:Transforming Growth Factor beta / Q000378:metabolism; D001748:Urinary Bladder Neck Obstruction / Q000000981:diagnostic imaging / Q000473:pathology / Q000628:therapy*
chemical_list: D005290:Ferric Compounds; D016212:Transforming Growth Factor beta; C000499:ferric oxide; D017228:Hepatocyte Growth Factor; D003094:Collagen; D019859:Proto-Oncogene Proteins c-met