title: A laminin-2-derived peptide promotes early-stage peripheral nerve regeneration in a dual-component artificial nerve graft.
abstract: The DLTIDDSYWYRI motif (Ln2-P3) of human laminin-2 has been reported to promote PC12 cell attachment through syndecan-1; however, the in vivo effects of Ln2-P3 have not been studied. In Schwann cells differentiated from skin-derived precursors, the peptide was effective in promoting cell attachment and spreading in vitro. To examine the effects of Ln2-P3 in peripheral nerve regeneration in vivo, we developed a dual-component poly(p-dioxanone) (PPD)/poly(lactic-co-glycolic acid) (PLGA) artificial nerve graft. The novel graft was coated with scrambled peptide or Ln2-P3 and used to bridge a 10 mm defect in rat sciatic nerves. The dual-component nerve grafts provided tensile strength comparable to that of a real rat nerve trunk. The Ln2-P3-treated grafts promoted early-stage peripheral nerve regeneration by enhancing the nerve regeneration rate and significantly increased the myelinated fibre density compared with scrambled peptide-treated controls. These findings indicate that Ln2-P3, combined with tissue-engineering scaffolds, has potential biomedical applications in peripheral nerve injury repair.
authors.affiliation: Department of Microbiology, Dong-A University College of Medicine, Busan, Republic of Korea.
authors.forename: S Y
authors.initials: SY
authors.lastname: Seo
authors.forename: S-K
authors.initials: SK
authors.lastname: Min
authors.forename: H K
authors.initials: HK
authors.lastname: Bae
authors.forename: D
authors.initials: D
authors.lastname: Roh
authors.forename: H K
authors.initials: HK
authors.lastname: Kang
authors.forename: S
authors.initials: S
authors.lastname: Roh
authors.forename: S
authors.initials: S
authors.lastname: Lee
authors.forename: G-S
authors.initials: GS
authors.lastname: Chun
authors.forename: D-J
authors.initials: DJ
authors.lastname: Chung
authors.forename: B-M
authors.initials: BM
authors.lastname: Min
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D004146:Dioxanes / Q000737:chemistry; D006801:Humans; D007150:Immunohistochemistry; D020007:Implants, Experimental; D007249:Inflammation / Q000473:pathology; D019344:Lactic Acid / Q000737:chemistry; D007797:Laminin / Q000737:chemistry* / Q000494:pharmacology; D008297:Male; D008969:Molecular Sequence Data; D009186:Myelin Sheath / Q000187:drug effects / Q000378:metabolism; D009416:Nerve Regeneration / Q000187:drug effects*; D058117:Neural Prostheses; D016716:PC12 Cells; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D010525:Peripheral Nerves / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology* / Q000648:ultrastructure; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000166:cytology / Q000187:drug effects; D013718:Tensile Strength / Q000187:drug effects
chemical_list: D004146:Dioxanes; D007797:Laminin; D010455:Peptides; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; C079733:poly-4-dioxan-2-one; D019344:Lactic Acid