title: [Survey of Theileria annulata and Theileria buffeli/orientalis complex in cattle in the Kırşehir region using multiplex-PCR].
abstract: OBJECTIVE
  To investigate the presence of T. annulata and T. buffeli/orientalis complex in the blood of 172 cattle selected from 9 different regions of Kırşehir.
  
  
  METHODS
  Genes for the merozoite surface antigen (Tams 1) and the major piroplasm surface protein (MPSP) were amplified with multiplex- PCR for T. annulata and T. buffeli/orientalis, respectively.
  
  
  RESULTS
  By multiplex-PCR examination 4 (2.32%) samples were positive for T. annulata whereas none of the samples were positive for T. buffeli/orientalis complex.
  
  
  CONCLUSIONS
  In this study, it is concluded that simultaneous diagnosis of Theileria species by using multiplex-PCR is more practical than the investigation of species individually by using classical PCR. We also believe that a more accurate epidemiological data is achieved with the use of moleculer diagnosis methods such as multiplex-PCR, which eliminates the disadvantages of the traditional methods used in the diagnosis of parasitic diseases.
authors.affiliation: Ankara Üniversitesi Veteriner Fakültesi, Parazitoloji Anabilim Dalı, Ankara, Türkiye, Turkey.
authors.forename: Ömer
authors.initials: Ö
authors.lastname: Orkun
authors.forename: Ahmet
authors.initials: A
authors.lastname: Deniz
authors.forename: Esin
authors.initials: E
authors.lastname: Güven
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics; D002417:Cattle; D016054:DNA, Protozoan / Q000097:blood / Q000302:isolation & purification*; D005260:Female; D008297:Male; D060885:Multiplex Polymerase Chain Reaction / Q000662:veterinary*; D015800:Protozoan Proteins / Q000235:genetics; D016794:Theileria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D016796:Theileria annulata / Q000235:genetics* / Q000302:isolation & purification; D013801:Theileriasis / Q000453:epidemiology / Q000469:parasitology*; D014421:Turkey / Q000453:epidemiology
chemical_list: D000953:Antigens, Protozoan; D016054:DNA, Protozoan; D015800:Protozoan Proteins; C115173:major piroplasm surface protein, Theileria