title: The SWI/SNF-like BAF complex is essential for early B cell development.
abstract: During the process of B cell development, transcription factors, such as E2A and Ebf1, have been known to play key roles. Although transcription factors and chromatin regulators work in concert to direct the expression of B lineage-specific genes, little is known about the involvement of regulators for chromatin structure during B lymphopoiesis. In this article, we show that deletion of Srg3/mBaf155, a scaffold subunit of the SWI/SNF-like BAF complex, in the hematopoietic lineage caused defects at both the common lymphoid progenitor stage and the transition from pre-pro-B to early pro-B cells due to failures in the expression of B lineage-specific genes, such as Ebf1 and Il7ra, and their downstream target genes. Moreover, mice that were deficient in the expression of Brg1, a subunit of the complex with ATPase activity, also showed defects in early B cell development. We also found that the expression of Ebf1 and Il7ra is directly regulated by the SWI/SNF-like BAF complex. Thus, our results suggest that the SWI/SNF-like BAF complex facilitates early B cell development by regulating the expression of B lineage-specific genes.
authors.affiliation: Department of Biological Sciences, Institute of Molecular Biology and Genetics, Research Center for Functional Cellulomics, Seoul National University, Seoul 151-742, Korea.
authors.forename: Jinwook
authors.initials: J
authors.lastname: Choi
authors.forename: Myunggon
authors.initials: M
authors.lastname: Ko
authors.forename: Shin
authors.initials: S
authors.lastname: Jeon
authors.forename: Yoon
authors.initials: Y
authors.lastname: Jeon
authors.forename: Kyungsoo
authors.initials: K
authors.lastname: Park
authors.forename: Changjin
authors.initials: C
authors.lastname: Lee
authors.forename: Ho
authors.initials: H
authors.lastname: Lee
authors.forename: Rho H
authors.initials: RH
authors.lastname: Seong
mesh_terms: D017502:Adenovirus E2 Proteins / Q000235:genetics / Q000276:immunology; D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000276:immunology; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology*; D019070:Cell Lineage / Q000276:immunology; D002843:Chromatin / Q000235:genetics / Q000276:immunology*; D002868:Chromosomal Proteins, Non-Histone / Q000172:deficiency / Q000235:genetics / Q000276:immunology*; D004265:DNA Helicases / Q000172:deficiency / Q000235:genetics / Q000276:immunology; D005786:Gene Expression Regulation; D051379:Mice; D018345:Mice, Knockout; D009687:Nuclear Proteins / Q000172:deficiency / Q000235:genetics / Q000276:immunology; D011070:Poly I-C / Q000494:pharmacology; D054448:Precursor Cells, B-Lymphoid / Q000166:cytology / Q000276:immunology*; D020395:Receptors, Interleukin-7 / Q000235:genetics / Q000276:immunology; D015398:Signal Transduction; D015534:Trans-Activators / Q000235:genetics / Q000276:immunology; D014157:Transcription Factors / Q000172:deficiency / Q000235:genetics / Q000276:immunology*
chemical_list: D017502:Adenovirus E2 Proteins; D051792:Basic Helix-Loop-Helix Proteins; D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D004265:DNA Helicases; D009687:Nuclear Proteins; D011070:Poly I-C; D020395:Receptors, Interleukin-7; D015534:Trans-Activators; D014157:Transcription Factors; C489921:Ebf1 protein, mouse; C445179:SWI-SNF-B chromatin-remodeling complex; C491090:Smarcc1 protein, mouse; C075668:Tcf3 protein, mouse; C513194:Smarca4 protein, mouse