title: Topical application of activity-based probes for visualization of brain tumor tissue.
abstract: Several investigators have shown the utility of systemically delivered optical imaging probes to image tumors in small animal models of cancer. Here we demonstrate an innovative method for imaging tumors and tumor margins during surgery. Specifically, we show that optical imaging probes topically applied to tumors and surrounding normal tissue rapidly differentiate between tissues. In contrast to systemic delivery of optical imaging probes which label tumors uniformly over time, topical probe application results in rapid and robust probe activation that is detectable as early as 5 minutes following application. Importantly, labeling is primarily associated with peri-tumor spaces. This methodology provides a means for rapid visualization of tumor and potentially infiltrating tumor cells and has potential applications for directed surgical excision of tumor tissues. Furthermore, this technology could find use in surgical resections for any tumors having differential regulation of cysteine cathepsin activity.
authors.affiliation: Department of Radiology, National Foundation for Cancer Research Center for Molecular Imaging at Case Western Reserve University, Cleveland, Ohio, United States of America.
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Cutter
authors.forename: Nathan T
authors.initials: NT
authors.lastname: Cohen
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Andrew E
authors.initials: AE
authors.lastname: Sloan
authors.forename: Alan R
authors.initials: AR
authors.lastname: Cohen
authors.forename: Ashok
authors.initials: A
authors.lastname: Panneerselvam
authors.forename: Mark
authors.initials: M
authors.lastname: Schluchter
authors.forename: Galia
authors.initials: G
authors.lastname: Blum
authors.forename: Matthew
authors.initials: M
authors.lastname: Bogyo
authors.forename: James P
authors.initials: JP
authors.lastname: Basilion
mesh_terms: D000287:Administration, Topical; D001932:Brain Neoplasms / Q000175:diagnosis* / Q000473:pathology / Q000601:surgery; D002403:Cathepsins / Q000378:metabolism; D045744:Cell Line, Tumor; D003545:Cysteine / Q000378:metabolism; D003952:Diagnostic Imaging; D005456:Fluorescent Dyes; D006801:Humans; D057054:Molecular Imaging / Q000295:instrumentation / Q000379:methods*; D016343:Monitoring, Intraoperative / Q000295:instrumentation / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D002403:Cathepsins; D003545:Cysteine