title: Multiplexing analysis of the polyspecific intrathecal immune response in multiple sclerosis.
abstract: Intrathecal synthesis of the antibodies specific to neurotrofic viruses: measles (M), rubella (R), Varicella-Zoster (Z), and/or H. simplex (H), known as "MRZH-reaction" plays important diagnostic role in multiple sclerosis (MS). Whereas the analysis of the oligoclonal IgG bands provides high sensitivity, the MRZH-reaction shows high specificity, and hence these methods complement each other. For the first time we applied multiplexing bead-based technology to simultaneously analyze cerebrospinal fluid (CSF) and serum concentrations of antibodies against these viruses, and to calculate the antibody specific indices (ASI's). The method shows reasonable precision: intra-assay, 2.9-6.7%, and inter-assay, 2.0-3.2%. The results are comparable with these obtained with other methods (ELISAs), including two runs of the certified external quality control schemes. Eighty-one percent of the MS cases (n=27) and none of the sex- and age-matched controls (n=14), except one subject with "borderline" anti-measles ASI of 1.5, showed intrathecal synthesis of IgG against at least one of the viruses discussed. The ratios of the MRZH-positive cases in the MS group were: 12/22 for M, 12/19 for R, 13/26 for Z, and 7/26 for H. We conclude that the multiplexing technology can be applied as a tool to study the intrathecal immune response in the diagnosis of MS.
authors.affiliation: Department of Neurology, Medical University of Białystok, Poland.
authors.forename: Alina
authors.initials: A
authors.lastname: Kułakowska
authors.forename: Barbara
authors.initials: B
authors.lastname: Mroczko
authors.forename: Maria
authors.initials: M
authors.lastname: Mantur
authors.forename: Natalia
authors.initials: N
authors.lastname: Lelental
authors.forename: Joanna
authors.initials: J
authors.lastname: Tarasiuk
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Kapica-Topczewska
authors.forename: Ute
authors.initials: U
authors.lastname: Schulz
authors.forename: Peter
authors.initials: P
authors.lastname: Lange
authors.forename: Rüdiger
authors.initials: R
authors.lastname: Zimmermann
authors.forename: Johannes
authors.initials: J
authors.lastname: Kornhuber
authors.forename: Piotr
authors.initials: P
authors.lastname: Lewczuk
mesh_terms: D000328:Adult; D000914:Antibodies, Viral / Q000097:blood* / Q000134:cerebrospinal fluid*; D015415:Biomarkers / Q000097:blood / Q000134:cerebrospinal fluid; D016022:Case-Control Studies; D005260:Female; D014645:Herpesvirus 3, Human / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000134:cerebrospinal fluid; D007163:Immunosorbent Techniques / Q000592:standards; D008297:Male; D008459:Measles virus / Q000276:immunology; D008863:Microspheres; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000175:diagnosis / Q000276:immunology* / Q000378:metabolism* / Q000821:virology; D011786:Quality Control; D011933:Reagent Kits, Diagnostic / Q000592:standards; D015203:Reproducibility of Results; D012412:Rubella virus / Q000276:immunology; D018139:Simplexvirus / Q000276:immunology; D012984:Software; D055815:Young Adult
chemical_list: D000914:Antibodies, Viral; D015415:Biomarkers; D007074:Immunoglobulin G; D011933:Reagent Kits, Diagnostic