title: Comparison of indirect and direct quantification of esters of monochloropropanediol in vegetable oil.
abstract: The presence of fatty acid esters of monochloropropanediol (MEs) in food is a recent concern raised due to the carcinogenicity of their hydrolysable moieties 2- and 3-monochloropropanediol (2- and 3-MCPD). Several indirect methods for the quantification of MEs have been developed and are commonly in use until today, however significant discrepancies among analytical results obtained are challenging their reliability. The aim of the present study was therefore to test the trueness of an indirect method by comparing it to a newly developed direct method using palm oil and palm olein as examples. The indirect method was based on ester cleavage under acidic conditions, derivatization of the liberated 2- and 3-MCPD with heptafluorobutyryl imidazole and GC-MS determination. The direct method was comprised of two extraction procedures targeting 2-and 3-MCPD mono esters (co-extracting as well glycidyl esters) by the use of double solid phase extraction (SPE), and 2- and 3-MCPD di-esters by the use of silica gel column, respectively. Detection was carried out by liquid chromatography coupled to time of flight mass spectrometry (LC-ToF-MS). Accurate quantification of the intact compounds was assured by means of matrix matched standard addition on extracts. Analysis of 22 palm oil and 7 palm olein samples (2- plus 3-MCPD contamination ranged from 0.3 to 8.8 μg/g) by both methods revealed no significant bias. Both methods were therefore considered as comparable in terms of results; however the indirect method was shown to require less analytical standards, being less tedious and furthermore applicable to all type of different vegetable oils and hence recommended for routine application.
authors.affiliation: Nestlé Research Centre, Nestec Ltd, Vers-chez-les-Blanc, 1000 Lausanne 26, Switzerland. mathieu.dubois@rdls.nestle.com
authors.forename: Mathieu
authors.initials: M
authors.lastname: Dubois
authors.forename: Adrienne
authors.initials: A
authors.lastname: Tarres
authors.forename: Till
authors.initials: T
authors.lastname: Goldmann
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Empl
authors.forename: Alfred
authors.initials: A
authors.lastname: Donaubauer
authors.forename: Walburga
authors.initials: W
authors.lastname: Seefelder
mesh_terms: D002853:Chromatography, Liquid; D004041:Dietary Fats / Q000032:analysis; D004075:Diglycerides / Q000032:analysis; D004952:Esters / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D005990:Glycerol / Q000031:analogs & derivatives* / Q000032:analysis; D013058:Mass Spectrometry; D010938:Plant Oils / Q000737:chemistry*; D052616:Solid Phase Extraction; D014280:Triglycerides / Q000032:analysis; D000517:alpha-Chlorohydrin
chemical_list: D004041:Dietary Fats; D004075:Diglycerides; D004952:Esters; D010938:Plant Oils; D014280:Triglycerides; D000517:alpha-Chlorohydrin; D005990:Glycerol