title: Synthesis, DNA binding, photo-induced DNA cleavage and cell cytotoxicity studies of a family of light rare earth complexes.
abstract: A family of light rare earth complexes, [RE(acac)(3)(dpq)] (RE=La (1), Ce (2), Pr (3), Nd (4), Sm (5)) and [RE(acac)(3)(dppz)].CH(3)OH (RE=La (6), Ce (7), Pr (8), Nd (9), Sm (10) viz. acetylacetonate (acac), dipyrido[3,2-d:20,30-f]quinoxaline (dpq), dipyrido[3,2-a:20,30-c] phenazine (dppz)), have been synthesized and structurally characterized. Binding interactions of these complexes with CT-DNA and their photo-induced DNA cleavage activity with pBR 322 DNA are also investigated. These complexes have strong DNA binding interaction (K(b)≈10(5)M(-1) and K(app)≈10(5)M(-1))and the binding propensity to CT-DNA decrease with the order: dppz complexes>dpq complexes. Furthermore, DNA photocleavage experiments indicate that these complexes are efficient DNA cleaving agents in UV-A (365 nm) and ambient light in the absence of any external reagents. Hydroxyl radical (HO(•)) and singlet oxygen ((1)O(2)) are the major cleavage active species from the machanistic studies. Moreover, cell cytotoxicity studies of these complexes on HeLa, K562 and MDA-MB-231 cells indicate that they have the potential to act as effective metal-based anti-cancer drugs.
authors.affiliation: Department of Chemistry, Nankai University, Tianjin 300071, PR China.
authors.forename: Gong-Jun
authors.initials: GJ
authors.lastname: Chen
authors.forename: Xin
authors.initials: X
authors.lastname: Qiao
authors.forename: Chun-Yan
authors.initials: CY
authors.lastname: Gao
authors.forename: Guang-Jun
authors.initials: GJ
authors.lastname: Xu
authors.forename: Zhi-Ling
authors.initials: ZL
authors.lastname: Wang
authors.forename: Jin-Lei
authors.initials: JL
authors.lastname: Tian
authors.forename: Jing-Yuan
authors.initials: JY
authors.lastname: Xu
authors.forename: Wen
authors.initials: W
authors.lastname: Gu
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Shi-Ping
authors.initials: SP
authors.lastname: Yan
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D056831:Coordination Complexes / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D018360:Crystallography, X-Ray; D004247:DNA / Q000378:metabolism* / Q000528:radiation effects; D006801:Humans; D008674:Metals, Rare Earth / Q000737:chemistry / Q000494:pharmacology*; D008958:Models, Molecular; D010619:Phenazines / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D010782:Photolysis; D011725:Pyridines / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D011810:Quinoxalines / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000970:Antineoplastic Agents; D056831:Coordination Complexes; D008674:Metals, Rare Earth; D010619:Phenazines; D011725:Pyridines; D011810:Quinoxalines; D004247:DNA