title: GlmS and NagB regulate amino sugar metabolism in opposing directions and affect Streptococcus mutans virulence.
abstract: Streptococcus mutans is a cariogenic pathogen that produces an extracellular polysaccharide (glucan) from dietary sugars, which allows it to establish a reproductive niche and secrete acids that degrade tooth enamel. While two enzymes (GlmS and NagB) are known to be key factors affecting the entrance of amino sugars into glycolysis and cell wall synthesis in several other bacteria, their roles in S. mutans remain unclear. Therefore, we investigated the roles of GlmS and NagB in S. mutans sugar metabolism and determined whether they have an effect on virulence. NagB expression increased in the presence of GlcNAc while GlmS expression decreased, suggesting that the regulation of these enzymes, which functionally oppose one another, is dependent on the concentration of environmental GlcNAc. A glmS-inactivated mutant could not grow in the absence of GlcNAc, while nagB-inactivated mutant growth was decreased in the presence of GlcNAc. Also, nagB inactivation was found to decrease the expression of virulence factors, including cell-surface protein antigen and glucosyltransferase, and to decrease biofilm formation and saliva-induced S. mutans aggregation, while glmS inactivation had the opposite effects on virulence factor expression and bacterial aggregation. Our results suggest that GlmS and NagB function in sugar metabolism in opposing directions, increasing and decreasing S. mutans virulence, respectively.
authors.affiliation: Department of Oral Microbiology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.
authors.forename: Miki
authors.initials: M
authors.lastname: Kawada-Matsuo
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Mazda
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Oogai
authors.forename: Mikihito
authors.initials: M
authors.lastname: Kajiya
authors.forename: Toshihisa
authors.initials: T
authors.lastname: Kawai
authors.forename: Sakuo
authors.initials: S
authors.lastname: Yamada
authors.forename: Shouichi
authors.initials: S
authors.lastname: Miyawaki
authors.forename: Takahiko
authors.initials: T
authors.lastname: Oho
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Komatsuzawa
mesh_terms: D019747:Aldose-Ketose Isomerases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D000606:Amino Sugars / Q000378:metabolism*; D018441:Biofilms / Q000254:growth & development; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D055786:Gene Knockout Techniques; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D005945:Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing) / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008954:Models, Biological; D009154:Mutation; D013295:Streptococcus mutans / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity*; D014774:Virulence / Q000235:genetics / Q000502:physiology; D037521:Virulence Factors / Q000235:genetics
chemical_list: D000606:Amino Sugars; D037521:Virulence Factors; D005945:Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing); C020582:glucosamine-6-phosphate isomerase; D019747:Aldose-Ketose Isomerases