title: Analysis of 8-hydroxy-2'-deoxyguanosine in human urine using hydrophilic interaction chromatography with tandem mass spectrometry.
abstract: Urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) is a widely used noninvasive biomarker of oxidative stress. A selective, sensitive and rapid method for determining 8-OHdG in human urine was developed using hydrophilic interaction chromatography-tandem mass spectrometry (HILIC-MS/MS) with electrospray ionization. 8-OHdG and isotopically labeled 8-OHdG (internal standard) were separated on a HILIC column with a mobile phase of 10 mM ammonium acetate: acetonitrile (1:9, v/v) within 10 min and detected by using a positive electrospray ionization interface under the selected reaction monitoring mode. The detection limits of 8-OHdG (corresponding to a signal-to-noise ratio of 3) for the HILIC-MS/MS system and the conventional method using a reversed-phase column with MS/MS were 1.0 and 26.0 fmol/injection, respectively. The proposed method makes it possible to monitor the basal level of urinary 8-OHdG from non-exposed healthy subjects and can be used for large-scale human studies.
authors.affiliation: Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.
authors.forename: Chiemi
authors.initials: C
authors.lastname: Hosozumi
authors.forename: Akira
authors.initials: A
authors.lastname: Toriba
authors.forename: Thanyarat
authors.initials: T
authors.lastname: Chuesaard
authors.forename: Takayuki
authors.initials: T
authors.lastname: Kameda
authors.forename: Ning
authors.initials: N
authors.lastname: Tang
authors.forename: Kazuichi
authors.initials: K
authors.lastname: Hayakawa
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000097:Acetonitriles; D002853:Chromatography, Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D057230:Limit of Detection; D015203:Reproducibility of Results; D059629:Signal-To-Noise Ratio; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000097:Acetonitriles; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine; C032159:acetonitrile