title: Dietary amino acid L-tryptophan requirement of fingerling Indian catfish, Heteropneustes fossilis (Bloch), estimated by growth and haemato-biochemical parameters.
abstract: An 8-week feeding trial was conducted to determine the dietary tryptophan requirement of fingerling Indian catfish, Heteropneustes fossilis (6.10 ± 1.15 cm, 4.44 ± 0.50 g). Six isonitrogenous (40 g 100 g⁻¹) and isoenergetic (17.90 kJ g⁻¹) amino acid test diets were formulated with gradation of 0.1 g 100 g⁻¹ containing graded levels of L-tryptophan (0.04-0.54 g 100 g⁻¹, dry diet). Fish were stocked in triplicate groups, in 75-L circular trough with flow-through system and fed experimental diets at 4% BW/day twice daily. Maximum live weight gain (258%), best feed conversion ratio (FCR) (1.54) and protein efficiency ratio (PER) (1.62) were obtained in fish fed diet containing 0.34 g 100 g⁻¹ tryptophan. However, quadratic regression analysis of weight gain, FCR, PER and body protein deposition (BPD) data indicated requirements for dietary tryptophan at 0.37, 0.33, 0.32 and 0.33 g 100 g⁻¹ of dry diet, respectively. Significantly (P < 0.05) higher body protein, minimum moisture and intermediate fat contents were recorded at 0.34 g 100 g⁻¹ dietary tryptophan diet. Ash content was not significantly different (P > 0.05) among treatments except for diets 0.04 and 0.14 g 100 g⁻¹. Excellent somatic and haematological indices values were obtained at the requirement level. Based on above results, it is recommended that the diet for H. fossilis should contain tryptophan at 0.32 g 100 g⁻¹, dry diet, corresponding to 0.80 g 100 g⁻¹ dietary protein for optimum growth and efficient feed utilization.
authors.affiliation: DST, Sponsored Fish Nutrition Research Laboratory, Department of Zoology, University of Kashmir, Hazratbal, Srinagar, 190006, J&K, India. imtiazamu1@yahoo.com.
authors.forename: Imtiaz
authors.initials: I
authors.lastname: Ahmed
mesh_terms: D000818:Animals; D017756:Aquaculture; D002397:Catfishes / Q000097:blood / Q000254:growth & development*; D004032:Diet; D014364:Tryptophan / Q000378:metabolism*
chemical_list: D014364:Tryptophan