title: [Cardiomycyte overexpression of miR-27b resulted in cardiac fibrosis and mitochondria injury in mice].
abstract: Previous microRNA (miRNA) array results have shown that the expression of miR-27b is upregulated in heart tissues from human cardiomyopathy and pressure-overloaded hypertrophic mouse model, implying that miR-27b might play an important role in heart diseases. To study the in vivo function of miR-27b, we generated a transgenic mouse line overexpressing miR-27b under the control of the 5.5 kb promoter of a-myosin heavy chain (a-MHC). Real-time PCR results demonstrated that miR-27b precursor and mature miR-27b were significantly increased in the heart tissues of miR-27b transgenic mice. miR-27b transgenic mice not only displayed cardiac hypertrophy, but also exhibited significant cardiac fibrosis. Further study showed that matrix metalloproteinase 13 (MMP13), a key regulator involved in cardiac fibrosis, was the target of miR-27b. The expression of MMP13 was decreased and the expression of Col I and III was increased in miR-27b transgenic mice.. In addition, defects in ultrastructral architecture were also found in miR-27b trans-genic mice. The above results demonstrated that miR-27b might promote cardiac fibrosis through inhibiting MMP13.
authors.affiliation: Genetic Laboratory of Development and Disease, State Key Laboratory of Proteomics, Institute of Biotechnology, Beijing 100071, China. houningzz@126.com
authors.forename: Ning
authors.initials: N
authors.lastname: Hou
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Zhen-Hua
authors.initials: ZH
authors.lastname: Li
authors.forename: Yang
authors.initials: Y
authors.lastname: Cao
authors.forename: Kai-Ji
authors.initials: KJ
authors.lastname: Fan
authors.forename: Xiao
authors.initials: X
authors.lastname: Yang
mesh_terms: D000818:Animals; D005260:Female; D005355:Fibrosis; D015870:Gene Expression; D053509:Matrix Metalloproteinase 13 / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D035683:MicroRNAs / Q000235:genetics*; D008928:Mitochondria / Q000235:genetics / Q000473:pathology*; D032383:Myocytes, Cardiac / Q000378:metabolism* / Q000473:pathology*; D011247:Pregnancy
chemical_list: C531388:MIRN27 microRNA, human; D035683:MicroRNAs; D053509:Matrix Metalloproteinase 13