title: A comparison of epidermal growth factor receptor expression in malignant peritoneal and pleural mesothelioma.
abstract: An evaluation of epidermal growth factor receptor (EGFR) phenotypic expression in malignant pleural and peritoneal mesothelioma was undertaken, using immunohistochemical (IHC) and fluorescence in situ hybridization (FISH) analysis. Thirty-eight malignant mesothelioma (MM) specimens were subjected to IHC staining and FISH to evaluate the expression of EGFR protein and gene status. Overall positive IHC reaction was detected in 20/38 (53%) cases, in 11/22 (50%) pleural MM, and in 9/16 (56%) peritoneal MM. Our study confirmed that EGFR membranous expression is a common feature in MM, but not in benign mesothelial lesion. Thirty-seven cases did not show a gene copy number gain. Only one case showed a copy number gain. The protein overexpression of EGFR was not related to a gene copy number gain.
authors.affiliation: Department of Diagnostic Pathology, Nara Medical University School of Medicine, Nara, Japan.
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Enomoto
authors.forename: Takahiko
authors.initials: T
authors.lastname: Kasai
authors.forename: Maiko
authors.initials: M
authors.lastname: Takeda
authors.forename: Masato
authors.initials: M
authors.lastname: Takano
authors.forename: Kouhei
authors.initials: K
authors.lastname: Morita
authors.forename: Eiji
authors.initials: E
authors.lastname: Kadota
authors.forename: Norishige
authors.initials: N
authors.lastname: Iizuka
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Maruyama
authors.forename: Joji
authors.initials: J
authors.lastname: Haratake
authors.forename: Yu
authors.initials: Y
authors.lastname: Kojima
authors.forename: Naoya
authors.initials: N
authors.lastname: Ikeda
authors.forename: Akitaka
authors.initials: A
authors.lastname: Nonomura
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism*; D004273:DNA, Neoplasm / Q000032:analysis; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism*; D005260:Female; D018628:Gene Dosage; D015972:Gene Expression Regulation, Neoplastic; D005819:Genetic Markers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008654:Mesothelioma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008875:Middle Aged; D010534:Peritoneal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D010997:Pleural Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm; D005819:Genetic Markers; D066246:ErbB Receptors