title: Cytotoxic effect and apoptosis induction by Bothrops leucurus venom lectin on tumor cell lines.
abstract: Neoplastic transformation is the abnormal proliferation of cells. These transformations are often related to changes in cell surface glycoconjugates which can be detected by lectins. We evaluated the anti-tumor potential of BlL, a galactoside-binding lectin isolated from Bothrops leucurus venom as well as its cytotoxicity and hemolysis activity. The phosphatidylserine externalization and mitochondrial membrane potential were also determined. BlL exhibited cytotoxic activity against all tumor cell lines tested by induced phosphatidylserine externalization and mitochondrial depolarization, indicating cell death by apoptosis.
authors.affiliation: Departamento de Bioquímica, Universidade Federal de Pernambuco, Avenida Prof. Arthur de Sá, s/n, Cidade Universitária, 50670-901, Recife, Pernambuco, Brazil.
authors.forename: Erika S
authors.initials: ES
authors.lastname: Nunes
authors.forename: Mary A A
authors.initials: MA
authors.lastname: Souza
authors.forename: Antônio F M
authors.initials: AF
authors.lastname: Vaz
authors.forename: Teresinha G
authors.initials: TG
authors.lastname: Silva
authors.forename: Jaciana S
authors.initials: JS
authors.lastname: Aguiar
authors.forename: André M
authors.initials: AM
authors.lastname: Batista
authors.forename: Maria M P
authors.initials: MM
authors.lastname: Guerra
authors.forename: Miriam C
authors.initials: MC
authors.lastname: Guarnieri
authors.forename: Luana C B B
authors.initials: LC
authors.lastname: Coelho
authors.forename: Maria T S
authors.initials: MT
authors.lastname: Correia
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D017837:Bothrops / Q000378:metabolism*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003435:Crotalid Venoms / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects; D037102:Lectins / Q000494:pharmacology*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D010718:Phosphatidylserines / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D003435:Crotalid Venoms; D037102:Lectins; D010718:Phosphatidylserines