title: [Degenerate oligonucleotide primed-PCR technology establishment and its sensitivity test analysis].
abstract: OBJECTIVE
  To establish a whole genome amplification testing system based on degenerate oligonucleotide primed-PCR (DOP-PCR) and to explore its reliability and sensitivity.
  
  
  METHODS
  DOP-PCR amplified production was detected by fluorescent labeled multiplex STR amplification and capillary electrophoresis detection system to determine reliability and sensitivity of DOP-PCR system.
  
  
  RESULTS
  DOP-PCR system was successfully established and the detection sensitivity reached 5 cells (30 pg) by pretreatment of DOP-PCR and then detection of STR genotyping.
  
  
  CONCLUSIONS
  The system established in this study is reliable and more testing sensitive for forensic trace evidence.
authors.affiliation: Department of Forensic Medicine, Hainan Medical College, Haikou 570102, China. forensicpatho@163.com
authors.forename: Jian-Qiang
authors.initials: JQ
authors.lastname: Deng
authors.forename: Bao-Qin
authors.initials: BQ
authors.lastname: Liu
authors.forename: Ji-Feng
authors.initials: JF
authors.lastname: Cai
authors.forename: Wen-Hui
authors.initials: WH
authors.lastname: Li
authors.forename: Ren
authors.initials: R
authors.lastname: Long
authors.forename: Yi-Ping
authors.initials: YP
authors.lastname: Hou
mesh_terms: D004247:DNA / Q000032:analysis* / Q000235:genetics*; D016172:DNA Fingerprinting / Q000379:methods; D017931:DNA Primers; D019075:Electrophoresis, Capillary; D005784:Gene Amplification; D005838:Genotype; D006801:Humans; D021141:Nucleic Acid Amplification Techniques; D009841:Oligonucleotides / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D020080:Tandem Repeat Sequences
chemical_list: D017931:DNA Primers; D009841:Oligonucleotides; D004247:DNA