title: Enrichment and purification of deoxyschizandrin and γ-schizandrin from the extract of Schisandra chinensis fruit by macroporous resins.
abstract: In present study, the performance and separation characteristics of 21 macroporous resins for the enrichment and purification of deoxyschizandrin and γ-schizandrin, the two major lignans from Schisandra chinensis extracts, were evaluated. According to our results, HPD5000, which adsorbs by the molecular tiers model, was the best macroporous resin, offering higher adsorption and desorption capacities and higher adsorption speed for deoxyschizandrin and γ-schizandrin than other resins. Columns packed with HPD5000 resin were used to perform dynamic adsorption and desorption tests to optimize the technical parameters of the separation process. The results showed that the best adsorption time is 4 h, the rate of adsorption is 0.85 mL/min (4 BV/h) and the rate of desorption is 0.43 mL/min (2 BV/h). After elution with 90% ethanol, the purity of deoxy-schizandrin increased 12.62-fold from 0.37% to 4.67%, the purity of γ-schizandrin increased 15.8-fold from 0.65% to 10.27%, and the recovery rate was more than 80%.
authors.affiliation: Key Laboratory of Forest Plant Ecology, Northeast Forestry University, Harbin 150040, China.
authors.forename: Feng-Jian
authors.initials: FJ
authors.lastname: Yang
authors.forename: Chun-Hui
authors.initials: CH
authors.lastname: Ma
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.forename: Chun-Jian
authors.initials: CJ
authors.lastname: Zhao
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yuan-Gang
authors.initials: YG
authors.lastname: Zu
mesh_terms: D000327:Adsorption; D034242:Cyclooctanes / Q000737:chemistry / Q000302:isolation & purification*; D005638:Fruit / Q000737:chemistry*; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification*; D011083:Polycyclic Compounds / Q000737:chemistry / Q000302:isolation & purification*; D012117:Resins, Synthetic / Q000737:chemistry*; D032141:Schisandra / Q000737:chemistry*
chemical_list: D034242:Cyclooctanes; D017705:Lignans; D011083:Polycyclic Compounds; D012117:Resins, Synthetic; C015499:schizandrin B; C034734:schizandrin A