title: Inhibitory Killer Immunoglobulin-like Receptors to self HLA-B and HLA-C ligands contribute differentially to Natural Killer cell functional potential in HIV infected slow progressors.
abstract: Inhibitory Killer Immunoglobulin-like Receptors (iKIR) interact with their ligands, HLA molecules, to license Natural Killer (NK) cells for functional competence. Previous studies stimulating peripheral blood mononuclear cells (PBMCs) with the HLA-devoid K562 cell line revealed that NK cells from individuals with an iKIR encoded by the KIR3DL1 locus with self HLA-Bw4 as their ligands, had higher frequencies of tri-functional NK cells that expressed the degranulation marker CD107a and secreted Interferon-γ and Tumor Necrosis Factor-α than those from individuals who were homozygous for HLA-Bw6 alleles, which are not ligands for these iKIR. To assess the effect of other iKIR to self-HLA (S-iKIR) on the NK cell response, we compared HIV-infected slow progressors (SP) carrying S-iKIR to HLA-C alleles with or without S-iKIR to HLA-Bw4. We show that S-iKIR to HLA-B and C alleles differ in their contribution to NK cell functional potential in HIV-infected SP upon stimulation with K562 targets.
authors.affiliation: Research Institute of the McGill University Health Centre, Montréal, Québec, Canada.
authors.forename: Philomena
authors.initials: P
authors.lastname: Kamya
authors.forename: Benjamin
authors.initials: B
authors.lastname: Tallon
authors.forename: Carlos
authors.initials: C
authors.lastname: Melendez-Pena
authors.forename: Matthew S
authors.initials: MS
authors.lastname: Parsons
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Migueles
authors.forename: Mark
authors.initials: M
authors.lastname: Connors
authors.forename: Sofia
authors.initials: S
authors.lastname: Miconiatis
authors.forename: Rujun
authors.initials: R
authors.lastname: Song
authors.forename: Salix
authors.initials: S
authors.lastname: Boulet
authors.forename: Julie
authors.initials: J
authors.lastname: Bruneau
authors.forename: Cecile L
authors.initials: CL
authors.lastname: Tremblay
authors.forename: Nicole F
authors.initials: NF
authors.lastname: Bernard
mesh_terms: D000328:Adult; D000368:Aged; D018791:CD4 Lymphocyte Count; D005260:Female; D015658:HIV Infections / Q000276:immunology*; D015235:HLA-B Antigens / Q000276:immunology*; D015236:HLA-C Antigens / Q000276:immunology*; D006801:Humans; D020014:K562 Cells; D007694:Killer Cells, Natural / Q000276:immunology*; D008875:Middle Aged; D054340:Receptors, KIR / Q000276:immunology*; D055815:Young Adult
chemical_list: D015235:HLA-B Antigens; C042388:HLA-Bw4 antigen; C038652:HLA-Bw6 antigen; D015236:HLA-C Antigens; D054340:Receptors, KIR