title: Association between the miRNA signatures in plasma and bronchoalveolar fluid in respiratory pathologies.
abstract: The identification of new less invasive biomarkers is necessary to improve the detection and prognostic outcome of respiratory pathological processes. The measurement of miRNA expression through less invasive techniques such as plasma and serum have been suggested to analysis of several lung malignancies including lung cancer. These studies are assuming a common deregulated miRNA expression both in blood and lung tissue. The present study aimed to obtain miRNA representative signatures both in plasma and bronchoalveolar cell fraction that could serve as biomarker in respiratory diseases. Ten patients were evaluated to assess the expression levels of 381 miRNAs. We found that around 50% miRNAs were no detected in both plasma and bronchoalveolar cell fraction and only 20% of miRNAs showed similar expression in both samples. These results show a lack of association of miRNA signatures between plasma and bronchoalveolar cytology in the same patient. The profiles are not comparable; however, there is a similarity in the relative expression in a very small subset of miRNAs (miR-17, miR-19b, miR-195 and miR-20b) between both biological samples in all patients. This finding supports that the miRNAs profiles obtained from different biological samples have to be carefully validated to link with respiratory diseases.
authors.affiliation: Department of Medical Oncology, University Hospital Virgen del Rocío, Instituto de Biomedicina de Sevilla, Seville, Spain.
authors.forename: Sonia
authors.initials: S
authors.lastname: Molina-Pinelo
authors.forename: Rocío
authors.initials: R
authors.lastname: Suárez
authors.forename: María Dolores
authors.initials: MD
authors.lastname: Pastor
authors.forename: Ana
authors.initials: A
authors.lastname: Nogal
authors.forename: Eduardo
authors.initials: E
authors.lastname: Márquez-Martín
authors.forename: José
authors.initials: J
authors.lastname: Martín-Juan
authors.forename: Amancio
authors.initials: A
authors.lastname: Carnero
authors.forename: Luis
authors.initials: L
authors.lastname: Paz-Ares
mesh_terms: D000230:Adenocarcinoma / Q000097:blood / Q000378:metabolism* / Q000473:pathology; D000368:Aged; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology*; D016022:Case-Control Studies; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D017563:Lung Diseases, Interstitial / Q000097:blood / Q000378:metabolism* / Q000473:pathology; D008175:Lung Neoplasms / Q000097:blood / Q000378:metabolism* / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D015415:Biomarkers; D035683:MicroRNAs