title: MiR-219-5p inhibits hepatocellular carcinoma cell proliferation by targeting glypican-3.
abstract: MicroRNAs (miRNAs) have been linked to the molecular pathogenesis of many cancers. In this study, we found that miR-219-5p was significantly downregulated in 83 HCC tissues and three HCC cell lines, compared to their non-tumor counterparts. MiR-219-5p expression correlated with tumor size, histological differentiation, and overall survival time in HCC patients. We also found that miR-219-5p could inhibit cell proliferation in vitro and arrest cell cycle at the G1 to S transition. Further studies identified that miR-219-5p reduced both the mRNA and protein levels of glypican-3 (GPC3). These findings indicate that miR-219-5p exerts tumor-suppressive effects in hepatic carcinogenesis through negative regulation of GPC3 expression.
authors.affiliation: Department of Oncology, Nanfang Hospital of Southern Medical University, Guangzhou 510515, Guangdong Province, China. lala343@126.com
authors.forename: Na
authors.initials: N
authors.lastname: Huang
authors.forename: Jing
authors.initials: J
authors.lastname: Lin
authors.forename: Jian
authors.initials: J
authors.lastname: Ruan
authors.forename: Ning
authors.initials: N
authors.lastname: Su
authors.forename: Ruzhai
authors.initials: R
authors.lastname: Qing
authors.forename: Feiye
authors.initials: F
authors.lastname: Liu
authors.forename: Benfu
authors.initials: B
authors.lastname: He
authors.forename: Chengwei
authors.initials: C
authors.lastname: Lv
authors.forename: Dayong
authors.initials: D
authors.lastname: Zheng
authors.forename: Rongcheng
authors.initials: R
authors.lastname: Luo
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology*; D002453:Cell Cycle / Q000502:physiology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D053673:Glypicans / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology*; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D015996:Survival Rate
chemical_list: D053673:Glypicans; D035683:MicroRNAs