title: A Toxoplasma gondii 10 kDa in vitro excretory secretory antigen reactive with human IgM and IgA antibodies.
abstract: Toxoplasmosis can cause serious disease in immunocompromised patients and to   congenitally infected foetuses. Appropriate laboratory investigations in potential cases of   acute Toxoplasma infection are important. Excretory secretory antigen (ESA) is immunogenic   during both human and experimental infections, therefore is considered as a good candidate   for investigation into new infection markers. In this study, ESA was prepared from in vitro   cultures of Toxoplasma gondii to identify T. gondii ESA antigenic component(s) that is/are   most reactive with serum samples from probable acute cases of toxoplasmosis. Serum samples   were obtained from several categories of individuals with the following Toxoplasma serology:   Group I: IgM+ IgG+ (low IgG avidity) or IgM+ IgG- from sera of patients who had clinical   query of toxoplasmosis (n=35). Group II: IgM- IgG+ (high IgG avidity) from chronically   infected individuals (n=30). Group III: normal/healthy individuals with anti-Toxoplasma IgMIgG-   (n=20). Group IV: individuals with other infections who had anti-Toxoplasma IgM- IgG-   (n=10). The ESA was subjected to SDS-PAGE, followed by Western blot analysis using the   above sera and probed with peroxidase conjugated anti-human IgM and IgA antibodies. The   blots were then developed using chemiluminescence substrate. The selected antigenic band   was excised from the gel after two dimensional electrophoresis and sent for mass spectrometry   analysis using MALDI TOF-TOF. The most promising antigenic band was a 10 kDa protein   which showed sensitivity of 80% in both IgM and IgA blots, and specificity of 96.7% with sera   from other infections and healthy controls. The two best identifications for the 10 kDa band   were ubiquitin (ribosomal protein CEP52 fusion protein) and polyubiquitin.
authors.affiliation: Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia.
authors.forename: G
authors.initials: G
authors.lastname: Saadatnia
authors.forename: F
authors.initials: F
authors.lastname: Ghaffarifar
authors.forename: A
authors.initials: A
authors.lastname: Khalilpour
authors.forename: A
authors.initials: A
authors.lastname: Amerizadeh
authors.forename: N
authors.initials: N
authors.lastname: Rahmah
mesh_terms: D000913:Antibodies, Protozoan / Q000097:blood*; D000953:Antigens, Protozoan / Q000302:isolation & purification; D015153:Blotting, Western / Q000379:methods; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood*; D007075:Immunoglobulin M / Q000097:blood*; D010274:Parasitology / Q000379:methods*; D012680:Sensitivity and Specificity; D014122:Toxoplasma / Q000276:immunology*; D014123:Toxoplasmosis / Q000175:diagnosis*
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D007070:Immunoglobulin A; D007075:Immunoglobulin M; C408624:excretory-secretory antigen, Toxoplasma