title: [Simultaneous determination of 11 phthalate metabolites in urine by liquid chromatography tandem mass spectrometry].
abstract: OBJECTIVE
  To develop a method for determining 11 kinds of phthalate metabolites in urine simultaneously.
  
  
  METHODS
  The phthalate metabolites in human urine samples were hydrolyzed by glucuronidase, and then were concentrated by solid-phase extraction and eluted by methanol/acetonitrile (1 : 1, V/V). After chromatographic separation on phenyl column, the analytes were analyzed qualitatively and quantitatively under MRM mode.
  
  
  RESULTS
  The limits of detection (LOD) for the eleven kinds of phthalate metabolites were in the range of 0.13 - 2.27 ng/ml. The mean recoveries at the two spiked levels were 67% - 108%, with the relative standard deviation (RSD) of less than 12%.
  
  
  CONCLUSIONS
  The method can be used to determine the residues of eleven kinds of phthalate metabolites in urine accurately and sensitively.
authors.affiliation: Institute for Environmental Health and Related Product Safety, China CDC, Beijing 100021, China. huxjian@yahoo.cn
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Hu
authors.forename: Miao
authors.initials: M
authors.lastname: Zhang
authors.forename: Xiaoya
authors.initials: X
authors.lastname: Xu
authors.forename: Changming
authors.initials: C
authors.lastname: Ding
authors.forename: Yinlong
authors.initials: Y
authors.lastname: Jin
authors.forename: Shaobin
authors.initials: S
authors.lastname: Lin
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D010795:Phthalic Acids / Q000378:metabolism* / Q000652:urine*; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D010795:Phthalic Acids; C032279:phthalic acid