title: The cloning of growth associated protein 43 of Gekko japonicus and its effect on cell morphology.
abstract: The growth-associated protein 43 (GAP-43) gene of Gekko japonicus was obtained from a brain and spinal cord cDNA library. The results of northern blot analysis showed the gecko GAP-43 gene transcript is 1.7 kb in length, and it was abundantly expressed in tissues of brain, spinal cord and ovary. Gecko GAP-43 promoted the outgrowth of Gsn3 cells and PC12 cell in vitro, and phosphorylation at serine 42 modulated the effect of GAP-43 on cell spreading and morphology. The change in GAP-43 expression in the spinal cord after tail amputation was examined by reverse transcription polymerase chain reaction (RT-PCR). The level of GAP-43 in the spinal cord was increased during the time course we examined, indicating a possible correlation between GAP-43 expression and the spinal cord injury and regeneration.
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, 226001 Jiangsu province, People's Republic of China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Feng
authors.forename: Youlang
authors.initials: Y
authors.lastname: Zhou
authors.forename: Mei
authors.initials: M
authors.lastname: Liu
authors.forename: Xingxing
authors.initials: X
authors.lastname: Gu
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Wang
authors.forename: Fei
authors.initials: F
authors.lastname: Ding
authors.forename: Xiaosong
authors.initials: X
authors.lastname: Gu
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D002460:Cell Line; D048430:Cell Shape; D003001:Cloning, Molecular; D019922:GAP-43 Protein / Q000235:genetics* / Q000378:metabolism*; D008116:Lizards / Q000235:genetics* / Q000378:metabolism; D009416:Nerve Regeneration; D012038:Regeneration; D013116:Spinal Cord / Q000378:metabolism; D013119:Spinal Cord Injuries / Q000235:genetics / Q000378:metabolism*; D013623:Tail
chemical_list: D019922:GAP-43 Protein