title: VP6-sequence-based cutoff values as a criterion for rotavirus species demarcation.
abstract: Indirect immunofluorescence techniques targeting the rotavirus (RV) protein VP6 are used to differentiate RV species. The ICTV recognizes RV species A to E and two tentative species, F and G. A potential new RV species, ADRV-N, has been described. Phylogenetic trees and pairwise identity frequency graphs were constructed with more than 400 available VP6 sequences and seven newly determined VP6 sequences of RVD strains. All RV species were separated into distinct phylogenetic clusters. An amino acid sequence cutoff value of 53% firmly permitted differentiation of RV species, and ADRV-N was tentatively assigned to a novel RV species H (RVH).
authors.affiliation: Laboratory of Clinical and Epidemiological Virology, Department of Microbiology and Immunology, Rega Institute for Medical Research, University of Leuven, Belgium. jelle.matthijnssens@uz.kuleuven.ac.be
authors.forename: Jelle
authors.initials: J
authors.lastname: Matthijnssens
authors.forename: Peter H
authors.initials: PH
authors.lastname: Otto
authors.forename: Max
authors.initials: M
authors.lastname: Ciarlet
authors.forename: Ulrich
authors.initials: U
authors.lastname: Desselberger
authors.forename: Marc
authors.initials: M
authors.lastname: Van Ranst
authors.forename: Reimar
authors.initials: R
authors.lastname: Johne
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000737:chemistry / Q000235:genetics*; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics*; D002645:Chickens; D008969:Molecular Sequence Data; D010802:Phylogeny; D011201:Poultry Diseases / Q000821:virology*; D012401:Rotavirus / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000662:veterinary* / Q000821:virology; D017386:Sequence Homology, Amino Acid
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; C061332:VP6 protein, Rotavirus