title: Detection of iron-labeled single cells by MR imaging based on intermolecular double quantum coherences at 14 T.
abstract: To evaluate the efficiency and feasibility of intermolecular multiple quantum coherence (iMQC) magnetic resonance (MR) imaging for single cell detection, we obtained intermolecular double quantum coherence (iDQC) and conventional gradient echo (GE) images of macrophage cells labeled by contrast agents in gel. The iDQC images obtained with echo-planar readout visualized the labeled cells effectively and with a higher contrast than seen in conventional GE images, especially at low planar resolutions and with thick slices. This implies that iDQC imaging with contrast agents could be a good alternative to conventional MR imaging for detecting labeled single cells or cell tracking under favorable conditions.
authors.affiliation: Division of Magnetic Resonance Research, Korea Basic Science Institute, Ochang 363-883, Republic of Korea.
authors.forename: Jee-Hyun
authors.initials: JH
authors.lastname: Cho
authors.forename: Kwan Soo
authors.initials: KS
authors.lastname: Hong
authors.forename: Janggeun
authors.initials: J
authors.lastname: Cho
authors.forename: Suk-Kyu
authors.initials: SK
authors.lastname: Chang
authors.forename: Chaejoon
authors.initials: C
authors.lastname: Cheong
authors.forename: Na Hee
authors.initials: NH
authors.lastname: Lee
authors.forename: Hyeonjin
authors.initials: H
authors.lastname: Kim
authors.forename: Warren S
authors.initials: WS
authors.lastname: Warren
authors.forename: Sangdoo
authors.initials: S
authors.lastname: Ahn
authors.forename: Chulhyun
authors.initials: C
authors.lastname: Lee
mesh_terms: D000818:Animals; D002460:Cell Line; D058948:Cell Tracking / Q000379:methods*; D003287:Contrast Media; D003911:Dextrans; D008264:Macrophages / Q000166:cytology*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles; D051379:Mice; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods
chemical_list: D003287:Contrast Media; D003911:Dextrans; D058185:Magnetite Nanoparticles; C065507:ferumoxides