title: Activity of superoxide dysmutase, catalase and glutathione peroxidase in rats exposed to chlorpyrifos and erofloxacin.
abstract: The aim of the study was to investigate the influence of administration of chlorpyrifos and/or enrofloxacin on the activity of chosen antioxidative enzymes i.e.: superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) in erythrocytes of rats. Chlorpyrifos was administered by stomach tube during 28 days at a dose of 3 mg/kg bw (0.02 LD50), and enrofloxacin was administered by stomach tube at a dose of 5 mg/kg bw during 3 subsequent days. It was stated that administration of enrofloxacin at applied dose did not cause any major changes in the activity of investigated antioxidative enzymes. The four-week exposure of rats to chlorpyrifos caused noticeable decrease in SOD and CAT activity in erythrocytes of rats at the beginning of the experiment (up to 24th hour) in comparison with the control group. The activity of GPx during all periods of the experiment was increased. In the group of animals in which both chlorpyrifos and enrofloxacin were applied, the profile of changes in activity of examined enzymes was similar to that one, which was observed after administration of chlorpyrifos exclusively, what may indicate lack of co-action between compounds used in the experiment.
authors.affiliation: Department Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 14, 10-719 Olsztyn, Poland. darbar@uwm.edu.pl
authors.forename: D
authors.initials: D
authors.lastname: Barski
authors.forename: A
authors.initials: A
authors.lastname: Spodniewska
authors.forename: A
authors.initials: A
authors.lastname: Zasadowski
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000633:toxicity; D002374:Catalase / Q000235:genetics / Q000378:metabolism*; D004390:Chlorpyrifos / Q000008:administration & dosage / Q000633:toxicity*; D004359:Drug Therapy, Combination; D000077422:Enrofloxacin; D004912:Erythrocytes / Q000201:enzymology; D024841:Fluoroquinolones / Q000008:administration & dosage / Q000633:toxicity*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism*; D007306:Insecticides / Q000008:administration & dosage / Q000633:toxicity; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D007306:Insecticides; D000077422:Enrofloxacin; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D004390:Chlorpyrifos