title: Isolation and characterization of 15 microsatellite loci in four endangered Amentotaxus species (Taxaceae).
abstract: OBJECTIVE
  Fifteen microsatellite loci were developed in an endangered species, Amentotaxus formosana, and were tested in an additional three species, A. argotaenia, A. yunnanensis, and A. poilanei, to evaluate the population structure for conservation efforts and reconstruct the phylogeographic patterns of this ancient lineage.
  
  
  RESULTS
  Polymorphic primer sets were developed from A. formosana; the number of alleles ranged from two to 10, with an observed heterozygosity ranging from 0 to 0.60. All of the loci were found to be interspecifically amplifiable.
  
  
  CONCLUSIONS
  These polymorphic and transferable loci will be potentially useful for future studies that will focus on identifying distinct genetic units within species and establishing the phylogeographic patterns and the process of speciation among closely related species.
authors.affiliation: Graduate Institute of Bioresources, National Pingtung University of Science and Technology, Pingtung, Taiwan.
authors.forename: Chin-Shang
authors.initials: CS
authors.lastname: Ho
authors.forename: Chaur-Tzuhn
authors.initials: CT
authors.lastname: Chen
authors.forename: Ya-Zhu
authors.initials: YZ
authors.lastname: Ko
authors.forename: Xue-Jun
authors.initials: XJ
authors.lastname: Ge
authors.forename: Kuo-Hsiang
authors.initials: KH
authors.lastname: Hung
authors.forename: Tsai-Wen
authors.initials: TW
authors.lastname: Hsu
authors.forename: Yu-Chung
authors.initials: YC
authors.lastname: Chiang
mesh_terms: D018744:DNA, Plant / Q000235:genetics* / Q000302:isolation & purification*; D056426:Genetic Loci / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D032490:Taxaceae / Q000235:genetics*
chemical_list: D018744:DNA, Plant