title: Capture of influenza viruses and prevention of their infection by coral mineral powder (sango mineral powder).
abstract: The anti-influenza virus activity of fossilized marine coral powder (sango mineral powder, SMP) was studied. SMP is composed in terms of mass of around 25 % of calcium and 10 % of magnesium, respectively, principally as dolomite (CaMg(CO(3))(2)) but not as calcium oxide (CaO) or magnesium oxide (MgO). By mixing the influenza virus with SMP, the infectivity of the virus substantially decreased and there was more than a 10(4) reduction on the 3rd d of infection. The antiviral effect was observed against all the type A and B strains of the influenza virus examined including the H1N1 2009 pandemic and H5N1 avian viruses. The surface structure of SMP was highly porous and the anti-influenza activity was explained by the adsorption of the viral particles onto its surface. The binding of viruses to SMP was strong and stable in the physiological condition, and the attached viruses detached only in the presence of a high concentration of phosphate. This was similar to the binding of protein to hydroxyapatite, suggesting an ionic interaction between SMP and the viral proteins. SMP maintained its activity to capture influenza viruses even after being immobilized on a non-woven textile. SMP would be useful as a practical anti-influenza tool especially in preparation for the next pandemic virus.
authors.affiliation: Department of Microbiology, Faculty of Bioscience, Nagahama Institute of Bio-Science and Technology, Nagahama, Shiga, Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Tachiki
authors.forename: Mika
authors.initials: M
authors.lastname: Kuramoto
authors.forename: Misuzu
authors.initials: M
authors.lastname: Kaneko
authors.forename: Mayuko
authors.initials: M
authors.lastname: Nawa
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Niwa
authors.forename: Masae
authors.initials: M
authors.lastname: Itoh
mesh_terms: D000327:Adsorption; D000818:Animals; D037421:Anthozoa; D000998:Antiviral Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D004285:Dogs; D008903:Minerals / Q000494:pharmacology*; D009975:Orthomyxoviridae / Q000187:drug effects* / Q000254:growth & development; D010316:Particle Size; D011208:Powders
chemical_list: D000998:Antiviral Agents; D008903:Minerals; D011208:Powders