title: Degradation of MONOCULM 1 by APC/C(TAD1) regulates rice tillering.
abstract: A rice tiller is a specialized grain-bearing branch that contributes greatly to grain yield. The MONOCULM 1 (MOC1) gene is the first identified key regulator controlling rice tiller number; however, the underlying mechanism remains to be elucidated. Here we report a novel rice gene, Tillering and Dwarf 1 (TAD1), which encodes a co-activator of the anaphase-promoting complex (APC/C), a multi-subunit E3 ligase. Although the elucidation of co-activators and individual subunits of plant APC/C involved in regulating plant development have emerged recently, the understanding of whether and how this large cell-cycle machinery controls plant development is still very limited. Our study demonstrates that TAD1 interacts with MOC1, forms a complex with OsAPC10 and functions as a co-activator of APC/C to target MOC1 for degradation in a cell-cycle-dependent manner. Our findings uncovered a new mechanism underlying shoot branching and shed light on the understanding of how the cell-cycle machinery regulates plant architecture.
authors.affiliation: State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Cao
authors.initials: C
authors.lastname: Xu
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Wang
authors.forename: Yanchun
authors.initials: Y
authors.lastname: Yu
authors.forename: Jingbo
authors.initials: J
authors.lastname: Duan
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Liao
authors.forename: Guosheng
authors.initials: G
authors.lastname: Xiong
authors.forename: Xiangbing
authors.initials: X
authors.lastname: Meng
authors.forename: Guifu
authors.initials: G
authors.lastname: Liu
authors.forename: Qian
authors.initials: Q
authors.lastname: Qian
authors.forename: Jiayang
authors.initials: J
authors.lastname: Li
mesh_terms: D064173:Anaphase-Promoting Complex-Cyclosome; D015820:Cadherins; D002453:Cell Cycle; D059447:Cell Cycle Checkpoints; D003001:Cloning, Molecular / Q000379:methods; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D012275:Oryza / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000378:metabolism; D043743:Ubiquitin-Protein Ligase Complexes / Q000235:genetics / Q000378:metabolism*
chemical_list: D015820:Cadherins; D010940:Plant Proteins; D043743:Ubiquitin-Protein Ligase Complexes; D064173:Anaphase-Promoting Complex-Cyclosome