title: Genome expression profile analysis of the immature maize embryo during dedifferentiation.
abstract: Maize is one of the most important cereal crops worldwide and one of the primary targets of genetic manipulation, which provides an excellent way to promote its production. However, the obvious difference of the dedifferentiation frequency of immature maize embryo among various genotypes indicates that its genetic transformation is dependence on genotype and immature embryo-derived undifferentiated cells. To identify important genes and metabolic pathways involved in forming of embryo-derived embryonic calli, in this study, DGE (differential gene expression) analysis was performed on stages I, II, and III of maize inbred line 18-599R and corresponding control during the process of immature embryo dedifferentiation. A total of ∼21 million cDNA tags were sequenced, and 4,849,453, 5,076,030, 4,931,339, and 5,130,573 clean tags were obtained in the libraries of the samples and the control, respectively. In comparison with the control, 251, 324 and 313 differentially expressed genes (DEGs) were identified in the three stages with more than five folds, respectively. Interestingly, it is revealed that all the DEGs are related to metabolism, cellular process, and signaling and information storage and processing functions. Particularly, the genes involved in amino acid and carbohydrate transport and metabolism, cell wall/membrane/envelope biogenesis and signal transduction mechanism have been significantly changed during the dedifferentiation. To our best knowledge, this study is the first genome-wide effort to investigate the transcriptional changes in dedifferentiation immature maize embryos and the identified DEGs can serve as a basis for further functional characterization.
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Maize Research Institute of Sichuan Agricultural University, Ministry of Agriculture, Wenjiang, People's Republic of China.
authors.forename: Yaou
authors.initials: Y
authors.lastname: Shen
authors.forename: Zhou
authors.initials: Z
authors.lastname: Jiang
authors.forename: Xiadong
authors.initials: X
authors.lastname: Yao
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Zhang
authors.forename: Haijian
authors.initials: H
authors.lastname: Lin
authors.forename: Maojun
authors.initials: M
authors.lastname: Zhao
authors.forename: Hailan
authors.initials: H
authors.lastname: Liu
authors.forename: Huanwei
authors.initials: H
authors.lastname: Peng
authors.forename: Shujun
authors.initials: S
authors.lastname: Li
authors.forename: Guangtang
authors.initials: G
authors.lastname: Pan
mesh_terms: D015415:Biomarkers / Q000378:metabolism*; D002454:Cell Differentiation; D018076:DNA, Complementary / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D018745:Genome, Plant; D020411:Oligonucleotide Array Sequence Analysis; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012639:Seeds / Q000166:cytology* / Q000378:metabolism*; D015398:Signal Transduction; D003313:Zea mays / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D018076:DNA, Complementary; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant