title: The cephalochordate amphioxus: a key to reveal the secrets of nuclear receptor evolution.
abstract: The members of the nuclear receptor (NR) superfamily are transcription factors characterized by a particular mode of function, which is related to the conserved nature of their molecular structure. NR proteins usually contain a DNA-binding domain (DBD) and a ligand-binding domain (LBD) allowing them to directly bind to DNA and regulate target gene expression in a ligand-dependent manner. In this review, we are summarizing our current understanding of the NR diversity in the cephalochordate amphioxus, which represents the best available proxy for the last common chordate ancestor both in terms of morphology and genome organization. The amphioxus genome encodes 33 NRs, which is more than expected based on its phylogenetic position, with at least one representative of all major NR groups, excepting NR1E and NR1I/J. This elevated number of receptor genes shows that the amphioxus NR complement has experienced some secondary modifications that are most evident in the NR1H group, which is characterized by three members in humans and ten representatives in amphioxus. By highlighting specific examples of the NR repertoire, including the receptors for retinoic acid, thyroid hormone, estrogen and steroids as well as the bile acid and oxysterol receptors of the NR1H group, we are illustrating the functional diversity of these receptors in amphioxus. We conclude that the amphioxus NRs are valuable models for assessing the evolutionary interplay between receptors and their ligands and that more integrative and comparative approaches are required for assessment of the evolutionary plasticity of receptor-ligand interactions revealed by the studies of amphioxus NRs.
authors.affiliation: Institut de Génomique Fonctionnelle de Lyon, UCBL, CNRS UMR, ENSL, INRA, Ecole Normale Supérieure de Lyon, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Lecroisey
authors.forename: Vincent
authors.initials: V
authors.lastname: Laudet
authors.forename: Michael
authors.initials: M
authors.lastname: Schubert
mesh_terms: D000818:Animals; D043344:Chordata / Q000235:genetics*; D017124:Conserved Sequence / Q000235:genetics; D019143:Evolution, Molecular; D014644:Genetic Variation; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics
chemical_list: D018160:Receptors, Cytoplasmic and Nuclear