title: Type-specific interaction between human papillomavirus type 58 E2 protein and E7 protein inhibits E7-mediated oncogenicity.
abstract: Human papillomavirus type 58 (HPV-58) is a very common HPV type in eastern Asia. Little is known about its biology and tumorigenesis. In this study, HPV-58 E2 protein (58E2) was found to interact with E7 protein (58E7), and the hinge domain of 58E2 was shown to be responsible for binding to the 58E7 protein. Interestingly, the E2-E7 interaction appears to be HPV type-specific, as we found that the HPV-16 E2 could not bind to the 58E7 protein, and neither did 58E2 interact with HPV-16 E7. The biological consequence(s) of the E2-E7 interaction in HPV-58, especially in viral tumorigenesis, was investigated. Results showed that, through interacting with 58E7, 58E2 prevented E7-induced retinoblastoma protein (pRb) degradation and prolonged the half-life of pRb in cells. Additionally, 58E2 abrogated 58E7-induced cell proliferation. These observations collectively suggest that direct interaction with 58E7 is another mechanism for 58E2 to inhibit 58E7-associated carcinogenesis in addition to regulating expression of the 58E7 gene.
authors.affiliation: Department of Microbiology, University of Pennsylvania School of Dental Medicine, Philadelphia, PA 19104, USA.|Department of Microbiology, Shandong University School of Medicine, Jinan, Shandong, 250012, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Microbiology, Shandong University School of Medicine, Jinan, Shandong, 250012, PR China.
authors.forename: Mei
authors.initials: M
authors.lastname: Qi
authors.affiliation: Department of Microbiology, Shandong University School of Medicine, Jinan, Shandong, 250012, PR China.
authors.forename: Xiuping
authors.initials: X
authors.lastname: Yu
authors.affiliation: Department of Microbiology, University of Pennsylvania School of Dental Medicine, Philadelphia, PA 19104, USA.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Department of Microbiology, Shandong University School of Medicine, Jinan, Shandong, 250012, PR China.
authors.forename: Weiming
authors.initials: W
authors.lastname: Zhao
mesh_terms: D001208:Asia; D049109:Cell Proliferation; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D006801:Humans; D009856:Oncogene Proteins, Viral / Q000378:metabolism*; D027383:Papillomaviridae / Q000472:pathogenicity*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D016160:Retinoblastoma Protein / Q000378:metabolism; D014759:Viral Envelope Proteins / Q000378:metabolism*
chemical_list: D009856:Oncogene Proteins, Viral; D016160:Retinoblastoma Protein; D014759:Viral Envelope Proteins