title: Mechanisms of protein kinase Sch9 regulating Bcy1 in Saccharomyces cerevisiae.
abstract: In this study, we investigated the mechanisms of Sch9 regulating the localization and phosphorylation of Bcy1. Our research indicated that Sch9 regulated localization of Bcy1 via Zds1 for the following reasons: (1) deletions of SCH9 or ZDS1 both caused nuclear localization of Bcy1; (2) Sch9 and Zds1 interacted physically; (3) overexpression of ZDS1 led to a significantly increased cytoplasmic localization of Bcy1 in sch9Δ cells, whereas overexpression of SCH9 had no visible effect on cytoplasmic localization of Bcy1 in zds1Δ cells. Our study also suggested that Sch9 regulated phosphorylation of Bcy1 via Yak1.
authors.affiliation: Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, China. zhangaili@tju.edu.cn
authors.forename: Aili
authors.initials: A
authors.lastname: Zhang
authors.forename: Wenxuan
authors.initials: W
authors.lastname: Gao
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D010766:Phosphorylation; D011485:Protein Binding; D025941:Protein Interaction Mapping; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C574570:Bcy1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C101740:ZDS1 protein, S cerevisiae; D017346:Protein Serine-Threonine Kinases; C530964:SCH9 protein, S cerevisiae; D017868:Cyclic AMP-Dependent Protein Kinases