title: Extrahepatic bile duct regeneration in pigs using collagen scaffolds loaded with human collagen-binding bFGF.
abstract: Extrahepatic bile duct defects and their complications are benign lesions but with malignant outcomes. Extrahepatic bile duct regeneration at the injury site could be important for the repair. In our previous work, a human basic fibroblast growth factor (bFGF) fused with a collagen-binding domain (CBD) was produced to activate the collagen membrane to obtain targeted tissue regeneration. This collagen/growth factor functional biomaterial could promote the regeneration of skin, bladder and full-thickness abdominal wall by accelerating vascularization and cellularization of autologous tissues. We speculate that the functional biomaterial could also provide the repairing effect on extrahepatic bile duct injuries. Using a pig extrahepatic bile duct injury model, we found that the collagen/CBD-bFGF composite biomaterial could significantly promote the extrahepatic bile duct regeneration at the injury site without causing structure deformation or hepatic dysfunction during both short- and long-time observations.
authors.affiliation: Department of Hepatobiliary Surgery, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.forename: Liang
authors.initials: L
authors.lastname: Tao
authors.forename: Bing
authors.initials: B
authors.lastname: Chen
authors.forename: Haozhen
authors.initials: H
authors.lastname: Ren
authors.forename: Xianglin
authors.initials: X
authors.lastname: Hou
authors.forename: Siqiao
authors.initials: S
authors.lastname: Zhou
authors.forename: Jianxin
authors.initials: J
authors.lastname: Zhou
authors.forename: Xitai
authors.initials: X
authors.lastname: Sun
authors.forename: Jianwu
authors.initials: J
authors.lastname: Dai
authors.forename: Yitao
authors.initials: Y
authors.lastname: Ding
mesh_terms: D000818:Animals; D017734:Bile Ducts, Extrahepatic / Q000000981:diagnostic imaging / Q000473:pathology / Q000502:physiology* / Q000601:surgery; D001663:Bilirubin / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D002417:Cattle; D002758:Cholangiography; D003094:Collagen / Q000378:metabolism / Q000494:pharmacology* / Q000648:ultrastructure; D003251:Constriction, Pathologic; D003893:Desmin / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D020007:Implants, Experimental; D053539:Keratin-19 / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008111:Liver Function Tests; D032389:Myocytes, Smooth Muscle / Q000187:drug effects / Q000378:metabolism; D018919:Neovascularization, Physiologic / Q000187:drug effects; D011183:Postoperative Complications / Q000209:etiology / Q000473:pathology; D011485:Protein Binding / Q000187:drug effects; D019651:Plastic Surgery Procedures; D012038:Regeneration / Q000187:drug effects*; D013194:Staining and Labeling; D034421:Sus scrofa; D013997:Time Factors; D054457:Tissue Scaffolds / Q000737:chemistry*; D014841:von Willebrand Factor / Q000378:metabolism
chemical_list: D003893:Desmin; D053539:Keratin-19; D014841:von Willebrand Factor; D016222:Fibroblast Growth Factor 2; D003094:Collagen; D001663:Bilirubin