title: Evaluation of multiplexed PCR and liquid-phase array for identification of respiratory fungal pathogens.
abstract: Invasive fungal infections are the cause of serious morbidity and high mortality in immunocompromised patients. Early laboratory diagnostic options remain limited; however, rapid detection and accurate identification may improve outcome. Herein, multiplexed PCR followed by liquid-phase array was evaluated for detection and identification of common respiratory fungal pathogens, including Aspergillus fumigatus, Rhizopus microsporus, Scedosporium apiospermum and Fusarium solani. The limit of detection ranged 0.1-1 ng of DNA, depending on the fungus being tested. Primer cross-reactivity was seen for some fungi: Aspergillus flavus primers detected Aspergillus oryzae; Scedosporium apiospermum primers detected Paecilomyces lilacinus, and Aspergillus terreus primers detected S. apiospermum. PCR followed by liquid-phase array is potentially useful for the identification of clinically relevant fungal pathogens.
authors.affiliation: Department of Pathology, St Jude Children's Research Hospital, Memphis, TN 38105 - 2794, USA.
authors.forename: Daelynn R
authors.initials: DR
authors.lastname: Buelow
authors.forename: Zhengming
authors.initials: Z
authors.lastname: Gu
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Walsh
authors.forename: Randall T
authors.initials: RT
authors.lastname: Hayden
mesh_terms: D003429:Cross Reactions; D017931:DNA Primers / Q000235:genetics; D005658:Fungi / Q000145:classification / Q000302:isolation & purification*; D006801:Humans; D046228:Microarray Analysis / Q000379:methods*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D009172:Mycology / Q000379:methods*; D009181:Mycoses / Q000175:diagnosis* / Q000382:microbiology; D012140:Respiratory Tract Diseases / Q000175:diagnosis* / Q000382:microbiology; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers