title: A novel intravital method to evaluate cerebral vasospasm in rat models of subarachnoid hemorrhage: a study with synchrotron radiation angiography.
abstract: Precise in vivo evaluation of cerebral vasospasm caused by subarachnoid hemorrhage has remained a critical but unsolved issue in experimental small animal models. In this study, we used synchrotron radiation angiography to study the vasospasm of anterior circulation arteries in two subarachnoid hemorrhage models in rats. Synchrotron radiation angiography, laser Doppler flowmetry-cerebral blood flow measurement, [(125)I]N-isopropyl-p-iodoamphetamine cerebral blood flow measurement and terminal examinations were applied to evaluate the changes of anterior circulation arteries in two subarachnoid hemorrhage models made by blood injection into cisterna magna and prechiasmatic cistern. Using synchrotron radiation angiography technique, we detected cerebral vasospasm in subarachnoid hemorrhage rats compared to the controls (p<0.05). We also identified two interesting findings: 1) both middle cerebral artery and anterior cerebral artery shrunk the most at day 3 after subarachnoid hemorrhage; 2) the diameter of anterior cerebral artery in the prechiasmatic cistern injection group was smaller than that in the cisterna magna injection group (p<0.05), but not for middle cerebral artery. We concluded that synchrotron radiation angiography provided a novel technique, which could directly evaluate cerebral vasospasm in small animal experimental subarachnoid hemorrhage models. The courses of vasospasm in these two injection models are similar; however, the model produced by prechiasmatic cistern injection is more suitable for study of anterior circulation vasospasm.
authors.affiliation: Department of Neurosurgery, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Cai
authors.forename: Yuhao
authors.initials: Y
authors.lastname: Sun
authors.forename: Falei
authors.initials: F
authors.lastname: Yuan
authors.forename: Lujia
authors.initials: L
authors.lastname: Chen
authors.forename: Chuan
authors.initials: C
authors.lastname: He
authors.forename: Yuhai
authors.initials: Y
authors.lastname: Bao
authors.forename: Zuoquan
authors.initials: Z
authors.lastname: Chen
authors.forename: Meiqing
authors.initials: M
authors.lastname: Lou
authors.forename: Weiliang
authors.initials: W
authors.lastname: Xia
authors.forename: Guo-Yuan
authors.initials: GY
authors.lastname: Yang
authors.forename: Feng
authors.initials: F
authors.lastname: Ling
mesh_terms: D000704:Analysis of Variance; D000792:Angiography / Q000379:methods*; D000818:Animals; D001921:Brain / Q000098:blood supply*; D002536:Cerebral Arteries / Q000473:pathology*; D007427:Intracranial Pressure; D007457:Iodine Radioisotopes; D020367:Iofetamine; D017078:Laser-Doppler Flowmetry / Q000379:methods; D051381:Rats; D012039:Regional Blood Flow; D013345:Subarachnoid Hemorrhage / Q000150:complications*; D017356:Synchrotrons; D020301:Vasospasm, Intracranial / Q000209:etiology / Q000473:pathology*
chemical_list: D007457:Iodine Radioisotopes; D020367:Iofetamine