title: Viability of postmortal epididymal mouse spermatozoa during long-term hypothermic storage and cryopreservation.
abstract: The study examined the effect of long-term hypothermic (4°C) storage of mouse carcasses on motility, cell membrane damage, in vitro survival and capacitation of epididymal spermatozoa before and after cryopreservation. It was shown that the number of spermatozoa with rectilinear forward motion decreased with increasing storage time. There were no significant changes in the total sperm motility and integrity of their plasmalemma. Pronounced effects of hypothermia and long-term storage of the mouse carcasses on cryocapacitation of spermatozoa during cryoconservation were demonstrated.
authors.affiliation: Laboratory of Genetic Resources Cryoconservation, Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, Russia.
authors.forename: N V
authors.initials: NV
authors.lastname: Shishova
authors.forename: E N
authors.initials: EN
authors.lastname: Gakhova
authors.forename: E V
authors.initials: EV
authors.lastname: Mel'nikova
mesh_terms: D020101:Acrosome Reaction; D000818:Animals; D002470:Cell Survival; D015925:Cryopreservation; D004822:Epididymis / Q000166:cytology*; D008297:Male; D051379:Mice; D013075:Sperm Capacitation; D013094:Spermatozoa / Q000166:cytology*