title: Association of LMP/TAP gene polymorphisms with tuberculosis susceptibility in Li population in China.
abstract: BACKGROUND
  Tuberculosis (TB) is a contagious disease affected by multiple genetic and environmental factors. Several association studies have suggested that cellular immune response is vital for controlling and preventing of tuberculosis infection. Low molecular weight polypeptides (LMPs) and transporters with antigen processing (TAPs) are the main molecules in the processing and presentation pathway for intracellular antigens. This study was performed to elucidate whether these antigen-processing genes (LMP/TAP) polymorphisms could be associated with the risk of tuberculosis infection in China.
  
  
  RESULTS
  We recruited 205 active pulmonary tuberculosis patients and 217 normal controls from Li population for this study. Four polymorphisms of LMP/TAP genes were determined by PCR-RFLP assay and haplotypes were constructed by software PHASE 1.0. Of the total four polymorphisms, genotype frequencies of LMP7 AA homozygote and CA heterozygote were significantly greater among cases compared to controls, with odds ratio of 3.77 (95% CI: 1.60-8.89; P = 0.002) and 2.97 (95% CI: 1.80-4.90; P<0.0001), respectively. The genotypes of TAP1-2 GG homozygote and AG heterozygote were more frequent in subjects with TB than in controls, with odds ratio of 3.94 (95% CI: 1.82-8.53; P = 0.001) and 2.87 (95% CI: 1.75-4.71; P<0.0001), respectively. Similarly, we found that haplotype B which carried LMP7 and TAP1-2 variations significantly increased the susceptibility to TB (OR = 3.674, 95% CI: 2.254-5.988; P<0.0001). Moreover, it is noteworthy that the homozygote of wild haplotype A (A/A) may be a strong protection for TB infection.
  
  
  CONCLUSIONS
  Our findings suggested that LMP/TAP gene polymorphisms might be risk factors for TB infection among Li population in China.
authors.affiliation: Laboratory of Human Function, Hainan Medical College, Haikou, Hainan Province, People's Republic of China.
authors.forename: Danmei
authors.initials: D
authors.lastname: Wang
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhou
authors.forename: Limin
authors.initials: L
authors.lastname: Ji
authors.forename: Tong
authors.initials: T
authors.lastname: He
authors.forename: Feng
authors.initials: F
authors.lastname: Lin
authors.forename: Rong
authors.initials: R
authors.lastname: Lin
authors.forename: Tangchang
authors.initials: T
authors.lastname: Lin
authors.forename: Yanna
authors.initials: Y
authors.lastname: Mo
mesh_terms: D000328:Adult; D017951:Antigen Presentation / Q000235:genetics; D002681:China / Q000453:epidemiology; D017931:DNA Primers / Q000235:genetics; D005006:Ethnicity / Q000235:genetics*; D005260:Female; D005802:Genes, MHC Class II / Q000235:genetics*; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D005843:Geography; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic / Q000235:genetics*; D014376:Tuberculosis / Q000453:epidemiology* / Q000235:genetics*
chemical_list: D017931:DNA Primers