title: Antiviral activity of crude extracts of Eugenia jambolana Lam. against highly pathogenic avian influenza (H5N1) virus.
abstract: Crude extracts of leaves and bark of E. jambolana were tested for antiviral activity against highly pathogenic avian influenza virus (H5N1) by CPE reduction assay in three different layouts to elucidate virucidal, post-exposure and preexposure antiviral activity of the extracts. The cold and hot aqueous extracts of bark and hot aqueous extract of leaves of E. jambolana showed significant virucidal activity (100% inhibition) which was further confirmed in virus yield reduction assay (-98 to 99% reduction) and by egg based in ovo assay. The selective index (CC50/EC50) of hot aqueous extract (248) and cold aqueous extract (43.5) of bark of E. jambolana showed their antiviral potential against H5N1 virus. The significant virucidal activity of leaves and bark of E. jambolana merits further investigation as it may provide alternative antiviral agent for managing avian influenza infections in poultry farms and potential avian-human transmission.
authors.affiliation: High Security Animal Disease Laboratory, Indian Veterinary Research Institute, Anand Nagar, Bhopal, Madhya Pradesh 462021, India. richa_bhatia@yahoo.com
authors.forename: Richa
authors.initials: R
authors.lastname: Sood
authors.forename: D
authors.initials: D
authors.lastname: Swarup
authors.forename: S
authors.initials: S
authors.lastname: Bhatia
authors.forename: D D
authors.initials: DD
authors.lastname: Kulkarni
authors.forename: S
authors.initials: S
authors.lastname: Dey
authors.forename: M
authors.initials: M
authors.lastname: Saini
authors.forename: S C
authors.initials: SC
authors.lastname: Dubey
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology*; D002460:Cell Line; D002645:Chickens; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000187:drug effects*; D007251:Influenza, Human / Q000517:prevention & control; D008826:Microbial Sensitivity Tests; D009976:Orthomyxoviridae Infections / Q000517:prevention & control; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D027842:Syzygium / Q000737:chemistry*
chemical_list: D000998:Antiviral Agents; D010936:Plant Extracts