title: A rapid and sensitive LC/ESI-MS/MS method for quantitative analysis of docetaxel in human plasma and its application to a pharmacokinetic study.
abstract: Docetaxel is a taxane family antineoplastic agent widely employed in cancer chemotherapy. We developed a liquid chromatography/tandem mass spectrometry method for the determination of docetaxel in human plasma. Plasma samples were deproteinized by acetonitrile containing internal standard paclitaxel. Chromatographic separation was performed on a TSKgel ODS-100 V 3 μm (50 mm × 2.0 mm i.d.) column using a mobile phase composed of acetonitrile-methanol-water-formic acid (50:5:45:0.1, v/v/v/v). Detection was performed on a triple-quadrupole tandem mass spectrometer with multiple reaction monitoring (MRM) mode via electrospray ionization (ESI) source. This method covered a linearity range of 5-5000 ng/mL with the lower limit of quantification of 5 ng/mL. The intra-day precision and inter-day precision (R.S.D.) of analysis were less than 6.7%, and the accuracy (R.E.) was within ± 9.0% at the concentrations of 5, 20, 200, and 2000 ng/mL. The total run time was 5.0 min. This method was successfully applied for clinical pharmacokinetic investigation.
authors.affiliation: Laboratory of Clinical Pharmaceutics & Therapeutics, Division of Pharmasciences, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo 060-0812, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Yamaguchi
authors.forename: Asuka
authors.initials: A
authors.lastname: Fujikawa
authors.forename: Hajime
authors.initials: H
authors.lastname: Ito
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Tanaka
authors.forename: Ayako
authors.initials: A
authors.lastname: Furugen
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Miyamori
authors.forename: Natsuko
authors.initials: N
authors.lastname: Takahashi
authors.forename: Jiro
authors.initials: J
authors.lastname: Ogura
authors.forename: Masaki
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Takehiro
authors.initials: T
authors.lastname: Yamada
authors.forename: Nariyasu
authors.initials: N
authors.lastname: Mano
authors.forename: Ken
authors.initials: K
authors.lastname: Iseki
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D000077143:Docetaxel; D004355:Drug Stability; D006258:Head and Neck Neoplasms / Q000097:blood* / Q000188:drug therapy / Q000378:metabolism; D006801:Humans; D016014:Linear Models; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D043823:Taxoids / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics / Q000627:therapeutic use
chemical_list: D043823:Taxoids; D000077143:Docetaxel