title: Competitive enzyme-linked immunoassay for sialoglycoprotein of edible bird's nest in food and cosmetics.
abstract: The proliferation of fake and inferior edible bird's nest (EBN) products has recently become an increasingly serious concern. To identify and classify EBN products, a competitive enzyme-linked immunoassay (ELISA) was developed to quantitate sialoglycoprotein in EBN used in food and cosmetic applications. The characteristic sialoglycoprotein in EBN was found, extracted, purified, and analyzed. Sialoglycoprotein, considered the main carrier of sialic acid in EBN, consisted of 106 and 128 kDa proteins. A monoclonal antibody that could recognize both proteins was prepared. The heat-treated process did not change the affinity of sialoglycoprotein with the antibody. An optimized ELISA method was established with a cross-reactivity of less than 0.1% and an IC(50) of 3.3 μg/mL. On the basis of different food and cosmetic samples, the limits of detection (LOD) were 10-18 μg/g. Recoveries of fortified samples at levels of 20 and 80 μg/g ranged from 81.5 to 96.5%, respectively. The coefficients of variation were less than 8.0%.
authors.affiliation: Shenzhen Academy of Metrology and Quality Inspection, Shenzhen 518102, People's Republic of China.
authors.forename: Shiwei
authors.initials: S
authors.lastname: Zhang
authors.forename: Xintian
authors.initials: X
authors.lastname: Lai
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Liu
authors.forename: Yun
authors.initials: Y
authors.lastname: Li
authors.forename: Bifang
authors.initials: B
authors.lastname: Li
authors.forename: Xiuli
authors.initials: X
authors.lastname: Huang
authors.forename: Qinlei
authors.initials: Q
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Lin
authors.initials: L
authors.lastname: Lin
authors.forename: Guowu
authors.initials: G
authors.lastname: Yang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001667:Binding, Competitive; D001717:Birds / Q000378:metabolism*; D003358:Cosmetics / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D012463:Saliva / Q000737:chemistry*; D012795:Sialoglycoproteins / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal; D003358:Cosmetics; D012795:Sialoglycoproteins