title: Whey protein coating bead improves the survival of the probiotic Lactobacillus rhamnosus CRL 1505 to low pH.
abstract: OBJECTIVE
  To evaluate the efficacy of a novel microencapsulation procedure using whey protein and pectin to improve the survival rate of Lactobacillus rhamnosus CRL 1505 to low pH and bile.
  
  
  RESULTS
  Lactobacillus rhamnosus CRL 1505 was encapsulated by ionotropic gelation using pectin (PE) and pectin-whey protein (PE-WP). Both types of beads (MC(PE/WP) and MC(PE-WP/WP)) were covered with a layer of whey protein by complex coacervation. The noncapsulated lactobacilli were not sensitive to bile salts but to acid. Both microparticles protected Lact. rhamnosus CRL 1505 at pH 2.0, but only MC(PE/WP) was effective at pH 1.2.
  
  
  CONCLUSIONS
  The combination of ionotropic gelation and complex coacervation techniques is efficient to obtain microcapsules of pectin covered with whey proteins. The MC(PE/WP) beads were more stable than the MC(PE-WP/WP) beads in simulated gastric conditions, thus offering better protection to Lact. rhamnosus CRL 1505 at low pH.
  
  
  CONCLUSIONS
  Pectin beads with a whey protein layer (MC(PE/WP)) could be used as probiotic carrier in functional foods of low pH (e.g. apple juice), thus protecting Lact. rhamnosus CRL 1505 against the stressful conditions of the gastric tract.
authors.affiliation: CERELA-Centro de Referencia para Lactobacilos, San Miguel de Tucumán, Argentina. gfont@cerela.org.ar
authors.forename: C L
authors.initials: CL
authors.lastname: Gerez
authors.forename: G
authors.initials: G
authors.lastname: Font de Valdez
authors.forename: M L
authors.initials: ML
authors.lastname: Gigante
authors.forename: C R F
authors.initials: CR
authors.lastname: Grosso
mesh_terms: D001646:Bile / Q000737:chemistry; D001647:Bile Acids and Salts / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry*; D004339:Drug Compounding / Q000379:methods*; D005750:Gastric Juice / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D052201:Lacticaseibacillus rhamnosus / Q000254:growth & development*; D008863:Microspheres; D008894:Milk Proteins / Q000737:chemistry*; D010368:Pectins / Q000737:chemistry*; D019936:Probiotics; D000067816:Whey Proteins
chemical_list: D001647:Bile Acids and Salts; D004337:Drug Carriers; D008894:Milk Proteins; D000067816:Whey Proteins; D010368:Pectins