title: Bacterial diversity associated with the rotifer Brachionus plicatilis sp. complex determined by culture-dependent and -independent methods.
abstract: The bacterial communities associated with rotifers (Brachionus plicatilis sp. complex) and their culture water were determined using culture-dependent and -independent methods (16S rRNA gene clone library). The bacterial communities determined by the culture-independent method were more diverse than those determined by the culture-dependent method. Although the culture-dependent method indicated the bacterial community of rotifers was relatively similar to that of the culture water, 16S rRNA gene clone library analyses revealed a great difference between the two microbiotas. Our results suggest that most bacteria associated with rotifers are not easily cultured using conventional methods, and that the microbiota of rotifers do not correspond with that of the culture water completely.
authors.affiliation: Laboratory of Marine Microbiology, Graduate School of Bioresources, Mie University, Mie, Japan.
authors.forename: Ryota
authors.initials: R
authors.lastname: Ishino
authors.forename: Shunpei
authors.initials: S
authors.lastname: Iehata
authors.forename: Miyo
authors.initials: M
authors.lastname: Nakano
authors.forename: Reiji
authors.initials: R
authors.lastname: Tanaka
authors.forename: Takao
authors.initials: T
authors.lastname: Yoshimatsu
authors.forename: Hiroto
authors.initials: H
authors.lastname: Maeda
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D003470:Culture Media; D010802:Phylogeny; D012403:Rotifera / Q000382:microbiology*
chemical_list: D003470:Culture Media