title: Hepatitis E virus replication requires an active ubiquitin-proteasome system.
abstract: The mechanism of hepatitis E virus (HEV) replication remains largely unknown. Here we demonstrate that HEV replication requires an active ubiquitin-proteasome system and that proteasome inhibitors affect HEV replication, possibly by inhibition of viral transcription or/and translation without a significant effect on cellular translation. Overexpression of ubiquitin in inhibitor-treated cells partially reverses the inhibitor effect on HEV replication. The results suggest that HEV replication requires interactions with proteasome machinery, which could be a potential therapeutic target against HEV.
authors.affiliation: Department of Biomedical Sciences and Pathobiology, College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA.
authors.forename: Yogesh A
authors.initials: YA
authors.lastname: Karpe
authors.forename: Xiang-Jin
authors.initials: XJ
authors.lastname: Meng
mesh_terms: D002460:Cell Line; D016751:Hepatitis E / Q000378:metabolism* / Q000821:virology*; D016752:Hepatitis E virus / Q000235:genetics / Q000502:physiology*; D006801:Humans; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D011485:Protein Binding; D025801:Ubiquitin / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D025801:Ubiquitin; D046988:Proteasome Endopeptidase Complex