title: Construction of a reporter system for Lactobacillus sp. using the gfpuv gene.
abstract: Lactobacilli are bacteria commonly associated with the gastrointestinal tract of animals and humans. They are able to produce antimicrobial substances such as bacteriocins, lactic acid and hydrogen peroxide, the factors which have been shown to be beneficial for controlling overgrowth of potentially pathogenic bacteria. For this reason lactobacilli are often applied in probiotics. The aim of present study was to construct a reporter strain based on the gfpuv expression system which can bee used as a strain with the ability to colonize the intestinal tract in future experiments.
authors.affiliation: Division of Bacteriology and Molecular Biology, Department of Preclinical Science, Faculty of Veterinary Medicine, Warsaw University of Life Sciences (SGGW), Ciszewskiego 8, 02-786 Warsaw, Poland.
authors.forename: T
authors.initials: T
authors.lastname: Garbaty
authors.forename: A
authors.initials: A
authors.lastname: Słońska
authors.forename: D
authors.initials: D
authors.lastname: Klimuszko
mesh_terms: D000818:Animals; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007778:Lactobacillus / Q000235:genetics* / Q000378:metabolism*
chemical_list: D049452:Green Fluorescent Proteins