title: Expression levels of vascular cell adhesion molecule-1 in young and nonobese women with polycystic ovary syndrome.
abstract: OBJECTIVE
  The objective of this study was to measure levels of mRNAs for inflammatory markers and resistin in human peripheral blood mononuclear cells (PBMCs) in young and nonobese women with polycystic ovary syndrome (PCOS).
  
  
  METHODS
  Fifteen young, nonobese women with PCOS and 10 age-matched controls were enrolled in this study. Levels of mRNAs for resistin and the inflammatory markers interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), monocyte chemoattractant protein-1 (MCP-1), intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) in human PBMCs were measured using real-time PCR.
  
  
  RESULTS
  The mean age and BMI of the women with PCOS were 27.54 ± 6.3 years and 27.4 ± 5.7, respectively. The women with PCOS had significantly higher fasting and 2-hour insulin levels, homeostasis model assessment of insulin resistance index (HOMA(IR)) and total cholesterol levels than the controls. VCAM-1 and ICAM-1 mRNA levels were significantly higher in women with PCOS than in controls, whereas no differences in resistin, IL-6, TNF-α and MCP-1 mRNA levels were observed between the groups. After adjusting for the BMI, only VCAM-1 mRNA levels were significantly higher in women with PCOS than in controls and correlated with the HOMA(IR) and total cholesterol.
  
  
  CONCLUSIONS
  Elevated VCAM-1 in human PBMCs in young, nonobese women with PCOS is associated with insulin resistance, independent of the BMI.
authors.affiliation: Department of Obstetrics and Gynecology, Shin Kong Wu Ho-Su Memorial Hospital, No. 95 Wen-Chang Road, Taipei, Taiwan.
authors.forename: Kok-Min
authors.initials: KM
authors.lastname: Seow
authors.forename: Chi-Chang
authors.initials: CC
authors.lastname: Juan
authors.forename: Peng-Hui
authors.initials: PH
authors.lastname: Wang
authors.forename: Low-Tone
authors.initials: LT
authors.lastname: Ho
authors.forename: Jiann-Loung
authors.initials: JL
authors.lastname: Hwang
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D015992:Body Mass Index; D016022:Case-Control Studies; D002784:Cholesterol / Q000097:blood; D016207:Cytokines / Q000378:metabolism; D005260:Female; D006801:Humans; D007249:Inflammation / Q000378:metabolism; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D009765:Obesity / Q000378:metabolism; D011085:Polycystic Ovary Syndrome / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D052243:Resistin / Q000378:metabolism; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism*; D055815:Young Adult
chemical_list: D015415:Biomarkers; D016207:Cytokines; D007328:Insulin; D012333:RNA, Messenger; D052243:Resistin; D019010:Vascular Cell Adhesion Molecule-1; D002784:Cholesterol