title: Diagnostic utility of components in allergy to Anisakis simplex.
abstract: BACKGROUND
  In our region, Anisakis allergy is responsible for 8% of acute urticarial reactions, 25% of which progress to anaphylactic shock. The poor specificity of skin tests and in vitro specific immunoglobulin (Ig) E means that Anisakis allergy is frequently overdiagnosed.
  
  
  OBJECTIVE
  We studied the diagnostic value of 2 Anisakis allergens: rAni s 1 and rAni s 3.
  
  
  METHODS
  Skin tests, the basophil activation test (BAT), and specific IgE determination were performed with rAni s 1 and 3 in 25 patients allergic to Anisakis, 17 atopic controls, and 10 controls with acute urticaria and positive skin test and sIgE results for Anisakis, but no allergy to Anisakis.
  
  
  RESULTS
  For rAni s1, skin tests had a sensitivity and specificity of 100% and specific IgE had a sensitivity and specificity of 100% in the atopic control group and 90% in the urticaria control group. BAT had a sensitivity of 96.8% and a specificity of 100% in the atopic control group and 66.7% in the urticaria control group. For rAni s 3, only 1 patient had positive specific IgE results to rAni s 3. All other techniques gave negative results in patients and controls
  
  
  CONCLUSIONS
  rAni s 1 is the major allergen of Anisakis and the target allergen when diagnosing allergy to Anisakis, rAni s 3 is not relevant when attempting to explain false-positive results.
authors.affiliation: Hospital de Basurto, Servicio de Alergologia, Bilbao, Spain. pedromanuel.gamboasetien@osakidetza.net
authors.forename: P M
authors.initials: PM
authors.lastname: Gamboa
authors.forename: J
authors.initials: J
authors.lastname: Asturias
authors.forename: R
authors.initials: R
authors.lastname: Martínez
authors.forename: I
authors.initials: I
authors.lastname: Antépara
authors.forename: I
authors.initials: I
authors.lastname: Jáuregui
authors.forename: I
authors.initials: I
authors.lastname: Urrutia
authors.forename: J
authors.initials: J
authors.lastname: Fernández
authors.forename: M L
authors.initials: ML
authors.lastname: Sanz
mesh_terms: D000328:Adult; D000485:Allergens / Q000276:immunology*; D000818:Animals; D017163:Anisakis / Q000276:immunology*; D000947:Antigens, Helminth / Q000276:immunology*; D002135:Calcium-Binding Proteins / Q000276:immunology*; D005260:Female; D015801:Helminth Proteins / Q000276:immunology*; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis*; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D008297:Male; D012680:Sensitivity and Specificity; D012882:Skin Tests; D014581:Urticaria / Q000175:diagnosis* / Q000276:immunology
chemical_list: D000485:Allergens; C553595:Ani s 3 allergen, Anisakis simplex; D000947:Antigens, Helminth; D002135:Calcium-Binding Proteins; D015801:Helminth Proteins; C412511:troponin-like protein, Anisakis simplex; D007073:Immunoglobulin E