title: Antioxidant peptides from protein hydrolysate of microalgae Navicula incerta and their protective effects in HepG2/CYP2E1 cells induced by ethanol.
abstract: Marine microalgae have been reported as valuable new sources of pharmacologically active compounds and there are now numerous commercial applications of microalgae. Hence, in this study we evaluated the protective effects of peptides purified from marine microalgae, Navicula incerta, against alcohol-induced damage in HepG2/CYP2E1 cells. To obtain bioactive peptides from microalgae, N. incerta was hydrolysed using various enzymes (alcalase, α-chymotrypsin, neutrase, papain, pepsin, pronase-E and trypsin), and the hydrolysates were evaluated for cytoprotective activity. Among them, papain-derived hydrolysate exhibited higher antioxidant activities than those of other enzymes. Therefore, papain hydrolysate was purified in order to obtain potent antihepatotoxic and antioxidative peptides. The amino acid sequences of the purified peptides were analysed as; NIPP-1 (Pro-Gly-Trp-Asn-Gln-Trp-Phe-Leu) with molecular mass 1 171 Da, and NIPP-2 (Val-Glu-Val-Leu-Pro-Pro-Ala-Glu-Leu) with molecular mass 1108 Da. Furthermore, this study demonstrated that NIPP-1 and NIPP-2 peptides inhibited ethanol-induced cytotoxicity in HepG2/CYP2E1 cells.
authors.affiliation: Marine Bioprocess Research Center, Pukyong National University, Busan, Republic of Korea.
authors.forename: Kyong-Hwa
authors.initials: KH
authors.lastname: Kang
authors.forename: Zhong-Ji
authors.initials: ZJ
authors.lastname: Qian
authors.forename: BoMi
authors.initials: B
authors.lastname: Ryu
authors.forename: Fatih
authors.initials: F
authors.lastname: Karadeniz
authors.forename: Daekyung
authors.initials: D
authors.lastname: Kim
authors.forename: Se-Kwon
authors.initials: SK
authors.lastname: Kim
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D002470:Cell Survival; D017377:Diatoms / Q000737:chemistry; D000431:Ethanol / Q000009:adverse effects; D056945:Hep G2 Cells; D006801:Humans; D058086:Microalgae / Q000737:chemistry*; D010455:Peptides / Q000494:pharmacology; D020011:Protective Agents / Q000494:pharmacology*; D011492:Protein Hydrolysates / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D010455:Peptides; D020011:Protective Agents; D011492:Protein Hydrolysates; D000431:Ethanol