title: Evaluation of a rapid immunochromatographic ODK-0901 test for detection of pneumococcal antigen in middle ear fluids and nasopharyngeal secretions.
abstract: Since the incidence of penicillin-resistant Streptococcus pneumoniae has been increasing at an astonishing rate throughout the world, the need for accurate and rapid identification of pneumococci has become increasingly important to determine the appropriate antimicrobial treatment. We have evaluated an immunochromatographic test (ODK-0901) that detects pneumococcal antigens using 264 middle ear fluids (MEFs) and 268 nasopharyngeal secretions (NPSs). A sample was defined to contain S. pneumoniae when optochin and bile sensitive alpha hemolytic streptococcal colonies were isolated by culture. The sensitivity and specificity of the ODK-0901 test were 81.4% and 80.5%, respectively, for MEFs from patients with acute otitis media (AOM). In addition, the sensitivity and specificity were 75.2% and 88.8%, respectively, for NPSs from patients with acute rhinosinusitis. The ODK-0901 test may provide a rapid and highly sensitive evaluation of the presence of S. pneumoniae and thus may be a promising method of identifying pneumococci in MEFs and NPSs.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Wakayama Medical University, Wakayama, Japan. mhotomi@wakayama-med.ac.jp
authors.forename: Muneki
authors.initials: M
authors.lastname: Hotomi
authors.forename: Akihisa
authors.initials: A
authors.lastname: Togawa
authors.forename: Shin
authors.initials: S
authors.lastname: Takei
authors.forename: Gen
authors.initials: G
authors.lastname: Sugita
authors.forename: Rinya
authors.initials: R
authors.lastname: Sugita
authors.forename: Masamitsu
authors.initials: M
authors.lastname: Kono
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Fujimaki
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Kamide
authors.forename: Akihiro
authors.initials: A
authors.lastname: Uchizono
authors.forename: Keiko
authors.initials: K
authors.lastname: Kanesada
authors.forename: Shoichi
authors.initials: S
authors.lastname: Sawada
authors.forename: Naohiro
authors.initials: N
authors.lastname: Okitsu
authors.forename: Yumi
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Yoko
authors.initials: Y
authors.lastname: Saijo
authors.forename: Noboru
authors.initials: N
authors.lastname: Yamanaka
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D001426:Bacterial Proteins / Q000235:genetics; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D002846:Chromatography, Affinity; D004432:Ear, Middle / Q000382:microbiology; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D009305:Nasopharynx / Q000378:metabolism*; D010034:Otitis Media with Effusion / Q000175:diagnosis* / Q000382:microbiology; D011008:Pneumococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012220:Rhinitis / Q000175:diagnosis* / Q000382:microbiology; D012680:Sensitivity and Specificity; D012852:Sinusitis / Q000175:diagnosis* / Q000382:microbiology; D013296:Streptococcus pneumoniae / Q000276:immunology / Q000302:isolation & purification*; D013682:Teichoic Acids / Q000276:immunology / Q000378:metabolism*; D055815:Young Adult
chemical_list: D001426:Bacterial Proteins; D012333:RNA, Messenger; D013682:Teichoic Acids; C051592:pneumococcal surface protein A