title: Involvement of CtBP2 in LPS-induced microglial activation.
abstract: CtBP2 (C-terminal binding protein 2), which is widely expressed during developmental processes and differentiation, acts as a transcriptional repressor following recruitment to target promoters through repressors or other co-repressor proteins. In this study, we elucidated the dynamic expression changes and localization of CtBP2 in lipopolysaccharide (LPS)-induced neuroinflammatory processes in adult rats. CtBP2 expression was strongly induced in active glia cells (microglia and astrocytes) in inflamed spinal cord. In vitro studies indicated that the up-regulation of CtBP2 may be involved in the subsequent microglia activation following LPS exposure. And the knock-down of CtBP2 in microglia cell line HAPI by siRNA showed that CtBP2 increased the activation of microglia induced by LPS. Collectively, these results suggested CtBP2 may be important in host defense in microglia-mediated immune response. Understanding the cell signal pathway may provide a novel strategy against inflammatory and immune reaction in neuroinflammation in central nervous system.
authors.affiliation: Department of Immunology, Medical College, Nantong University, 19 Qixiu Road, Nantong 226001, People's Republic of China.
authors.forename: Guowei
authors.initials: G
authors.lastname: Zhang
authors.forename: Yaohua
authors.initials: Y
authors.lastname: Yan
authors.forename: Lihua
authors.initials: L
authors.lastname: Kang
authors.forename: Qi
authors.initials: Q
authors.lastname: Cao
authors.forename: Kaifu
authors.initials: K
authors.lastname: Ke
authors.forename: Xinmin
authors.initials: X
authors.lastname: Wu
authors.forename: Yilu
authors.initials: Y
authors.lastname: Gao
authors.forename: Qinglei
authors.initials: Q
authors.lastname: Hang
authors.forename: Chunmiao
authors.initials: C
authors.lastname: Li
authors.forename: Lin
authors.initials: L
authors.lastname: Zhu
authors.forename: Qin
authors.initials: Q
authors.lastname: Yuan
authors.forename: Qiyun
authors.initials: Q
authors.lastname: Wu
authors.forename: Chun
authors.initials: C
authors.lastname: Cheng
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D015153:Blotting, Western; D002460:Cell Line; D005136:Eye Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D020868:Gene Silencing; D007249:Inflammation / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism*; D007278:Injections, Spinal; D008070:Lipopolysaccharides / Q000008:administration & dosage; D017628:Microglia / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D034741:RNA, Small Interfering / Q000235:genetics; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D013116:Spinal Cord / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D014162:Transfection
chemical_list: C486016:Ctbp2 protein, rat; D005136:Eye Proteins; D008070:Lipopolysaccharides; D034741:RNA, Small Interfering