title: Microbial communities in long-term, water-flooded petroleum reservoirs with different in situ temperatures in the Huabei Oilfield, China.
abstract: The distribution of microbial communities in the Menggulin (MGL) and Ba19 blocks in the Huabei Oilfield, China, were studied based on 16S rRNA gene analysis. The dominant microbes showed obvious block-specific characteristics, and the two blocks had substantially different bacterial and archaeal communities. In the moderate-temperature MGL block, the bacteria were mainly Epsilonproteobacteria and Alphaproteobacteria, and the archaea were methanogens belonging to Methanolinea, Methanothermobacter, Methanosaeta, and Methanocella. However, in the high-temperature Ba19 block, the predominant bacteria were Gammaproteobacteria, and the predominant archaea were Methanothermobacter and Methanosaeta. In spite of shared taxa in the blocks, differences among wells in the same block were obvious, especially for bacterial communities in the MGL block. Compared to the bacterial communities, the archaeal communities were much more conserved within blocks and were not affected by the variation in the bacterial communities.
authors.affiliation: Department of Energy and Resources Engineering, College of Engineering, Peking University, Beijing, People's Republic of China.
authors.forename: Yue-Qin
authors.initials: YQ
authors.lastname: Tang
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Jie-Yu
authors.initials: JY
authors.lastname: Zhao
authors.forename: Chang-Qiao
authors.initials: CQ
authors.lastname: Chi
authors.forename: Li-Xin
authors.initials: LX
authors.lastname: Huang
authors.forename: Han-Ping
authors.initials: HP
authors.lastname: Dong
authors.forename: Xiao-Lei
authors.initials: XL
authors.lastname: Wu
mesh_terms: D001105:Archaea / Q000145:classification / Q000235:genetics / Q000254:growth & development*; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development*; D001483:Base Sequence; D002681:China; D016000:Cluster Analysis; D015723:Gene Library; D008969:Molecular Sequence Data; D061329:Oil and Gas Fields / Q000382:microbiology*; D010578:Petroleum / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013696:Temperature; D013997:Time Factors; D014871:Water Microbiology
chemical_list: D010578:Petroleum; D012336:RNA, Ribosomal, 16S