title: Detection of Actinobacillus pleuropneumoniae in pigs by real-time quantitative PCR for the apxIVA gene.
abstract: A real-time quantitative PCR (qPCR) for detection of the apxIVA gene of Actinobacillus pleuropneumoniae was validated using pure cultures of A. pleuropneumoniae and tonsillar and nasal swabs from experimentally inoculated Caesarean-derived/colostrum-deprived piglets and naturally infected conventional pigs. The analytical sensitivity was 5colony forming units/reaction. In comparison with selective bacterial examination using tonsillar samples from inoculated animals, the diagnostic sensitivity of the qPCR was 0.98 and the diagnostic specificity was 1.0. The qPCR showed consistent results in repeatedly sampled conventional pigs. Tonsillar brush samples and apxIVA qPCR analysis may be useful for further epidemiological studies and monitoring for A. pleuropneumoniae.
authors.affiliation: Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 7, 3584 CL, Utrecht, The Netherlands. T.J.Tobias@uu.nl
authors.forename: T J
authors.initials: TJ
authors.lastname: Tobias
authors.forename: A
authors.initials: A
authors.lastname: Bouma
authors.forename: D
authors.initials: D
authors.lastname: Klinkenberg
authors.forename: A J J M
authors.initials: AJ
authors.lastname: Daemen
authors.forename: J A
authors.initials: JA
authors.lastname: Stegeman
authors.forename: J A
authors.initials: JA
authors.lastname: Wagenaar
authors.forename: B
authors.initials: B
authors.lastname: Duim
mesh_terms: D000189:Actinobacillus Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D016977:Actinobacillus pleuropneumoniae / Q000235:genetics* / Q000302:isolation & purification; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D002585:Cesarean Section / Q000662:veterinary; D003126:Colostrum / Q000382:microbiology; D009666:Nose / Q000382:microbiology; D014066:Palatine Tonsil / Q000382:microbiology; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods* / Q000662:veterinary; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000382:microbiology
chemical_list: C122177:ApxIVA protein, Actinobacillus pleuropneumoniae; D001426:Bacterial Proteins