title: Specific domains of FoxD4/5 activate and repress neural transcription factor genes to control the progression of immature neural ectoderm to differentiating neural plate.
abstract: FoxD4/5, a forkhead transcription factor, plays a critical role in establishing and maintaining the embryonic neural ectoderm. It both up-regulates genes that maintain a proliferative, immature neural ectoderm and down-regulates genes that promote the transition to a differentiating neural plate. We constructed deletion and mutant versions of FoxD4/5 to determine which domains are functionally responsible for these opposite activities, which regulate the critical developmental transition of neural precursors to neural progenitors to differentiating neural plate cells. Our results show that up-regulation of genes that maintain immature neural precursors (gem, zic2) requires the Acidic blob (AB) region in the N-terminal portion of the protein, indicating that the AB is the transactivating domain. Additionally, down-regulation of those genes that promote the transition to neural progenitors (sox) and those that lead to neural differentiation (zic, irx) involves: 1) an interaction with the Groucho co-repressor at the Eh-1 motif in the C-terminus; and 2) sequence downstream of this motif. Finally, the ability of FoxD4/5 to induce the ectopic expression of neural precursor genes in the ventral ectoderm also involves both the AB region and the Eh-1 motif; FoxD4/5 accomplishes ectopic neural induction by both activating neural precursor genes and repressing BMP signaling and epidermal genes. This study identifies the specific, conserved domains of the FoxD4/5 protein that allow this single transcription factor to regulate a network of genes that controls the transition of a proliferative neural ectodermal population to a committed neural plate population poised to begin differentiation.
authors.affiliation: Department of Anatomy and Regenerative Biology, George Washington University School of Medicine and Health Sciences, 2300 I Street NW, Washington DC, USA.
authors.forename: Karen M
authors.initials: KM
authors.lastname: Neilson
authors.forename: Steven L
authors.initials: SL
authors.lastname: Klein
authors.forename: Pallavi
authors.initials: P
authors.lastname: Mhaske
authors.forename: Kathy
authors.initials: K
authors.lastname: Mood
authors.forename: Ira O
authors.initials: IO
authors.lastname: Daar
authors.forename: Sally A
authors.initials: SA
authors.lastname: Moody
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002454:Cell Differentiation; D004475:Ectoderm / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D051858:Forkhead Transcription Factors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D054258:Neural Plate / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D017434:Protein Structure, Tertiary; D015533:Transcriptional Activation; D014981:Xenopus; D029867:Xenopus Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: C470165:FOXD4 protein, human; D051858:Forkhead Transcription Factors; C495064:FoxD5a protein, Xenopus; D029867:Xenopus Proteins