title: [Establishment of the inferior alveolar nerve transection combined experimental periodontitis animal modal in rats].
abstract: OBJECTIVE
  To establish an animal model of the inferior alveolar nerve (IAN) transected combined experimental periodontitis in rats, in order to provide a foundation for exploring the function of nerve factor in the occurrence and development of periodontitis and periodontal tissue regeneration in vivo.
  
  
  METHODS
  Thirty-six adult SPF Wistar rats were divided into 3 groups randomly. The IAN-transection and periodontitis group (P1): steel-wire was used to ligate to the dental cervix of the dual first lower molars, the gingiva was lacerated using a dental probe, and the surgical transection of the IAN-transection was applied to the left side of the experimental rats, the animals were provided with high-carbonhydrate diet after surgery;the IAN-transection group (P2): the surgical transection of the IAN-transection was applied to the left side of the experimental group, the animals were fed on in routine way after surgery; the sham surgical group(N): no surgery was performed, the animals were fed on in routine way after surgery.6 weeks after the operation, specimens were collected by dissecting the operation areas after internal fixation and observed by clinical examination, X-ray, and histological examination. SPSS13.0 software package were used for statistical analysis.
  
  
  RESULTS
  The IAN-transection operation was successfully conducted in the study and there was no significant necrosis or ulceration observed in the nerve dissected rats. All experimental periodontitis models were established successfully at the time point; there was severe destruction of the periodontal tissues especially in the P1 group. There was bone losses in the specimens of IAN-transection side than that in non IAN-transection side according to X-ray examination 6 weeks after operation both in the P1 and P2 group, especially in the P1 group. Periodontal tissues on both sides of the P2 group had no obvious inflammation and periodontal destruction.
  
  
  CONCLUSIONS
  The experimental periodontitis animal model in various nerve conditions can be successfully conducted. The inferior alveolar nerve transection can result in severe destruction of the periodontal tissues, but it can not result in inflammation of normal periodontal tissue.
authors.affiliation: Department of Periodontics, Shandong University, Shandong Provincial Key Laboratory of Oral Biomedicine. Jinan,China. sunrubing6721@sina.com
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.forename: Shu
authors.initials: S
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Gao
authors.forename: Pan-pan
authors.initials: PP
authors.lastname: Zhang
authors.forename: Pi-shan
authors.initials: PS
authors.lastname: Yang
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D008340:Mandibular Nerve; D008963:Molar; D010518:Periodontitis; D010519:Periodontium; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D014945:Wound Healing