title: Chemical composition, antimicrobial, antiradical and anticholinesterase activity of the essential oil of Pulicaria stephanocarpa from Soqotra.
abstract: The chemical composition of the hydrodistilled leaf essential oil from Pulicaria stephanocarpa Balf. Fil was determined by GC-MS analysis, and its antimicrobial, antioxidant and anticholinesterase (AChE) activities were evaluated. Eighty-three compounds were identified representing 97.2% of the total oil. (E)-Caryophyllene 13.4%, (E)-nerolidol 8.5%, caryophyllene oxide 8.5%, alpha-cadinol 8.2% spathulenol 6.8% and tau-cadinol 4.7%, were the main components. Antimicrobial activity of the oil, evaluated using the disc diffusion and broth dilution methods, demonstrated the highest susceptibility on Gram-positive bacteria and Candida albicans. The free radical scavenging ability of the oil was assessed by the DPPH assay to show antiradical activity with IC50 of 330 microg/mL. Moreover, the oil revealed an AChE inhibitory activity of 47% at a concentration of 200 microg/mL using Ellman's method.
authors.affiliation: Pharmacognosy Department, Faculty of Pharmacy, Sana'a University, P. O. Box 13150, Sana'a, Yemen. alinasser9678@yahoo.com
authors.forename: Nasser A Awadh
authors.initials: NA
authors.lastname: Ali
authors.forename: Rebecca A
authors.initials: RA
authors.lastname: Crouch
authors.forename: Mohamed A
authors.initials: MA
authors.lastname: Al-Fatimi
authors.forename: Norbert
authors.initials: N
authors.lastname: Arnold
authors.forename: Axel
authors.initials: A
authors.lastname: Teichert
authors.forename: William N
authors.initials: WN
authors.lastname: Setzer
authors.forename: Ludger
authors.initials: L
authors.lastname: Wessjohann
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D008401:Gas Chromatography-Mass Spectrometry; D009822:Oils, Volatile / Q000032:analysis* / Q000494:pharmacology*; D052587:Pulicaria / Q000737:chemistry*
chemical_list: D000890:Anti-Infective Agents; D002800:Cholinesterase Inhibitors; D016166:Free Radical Scavengers; D009822:Oils, Volatile