title: A brief report on some health aspects of rats fed with crescent levels of recombinant chagasin, a potential plant defense protein.
abstract: Chagasin may be considered a potential plant-incorporated protectant (PIP) protein due to its deleterious effects on insect pests. However, extensive safety studies with PIP's are necessary before introducing them into the target plant. Thus, a short-term feeding trial in rats with high doses of r-chagasin was conducted to provide evidences about its safety. Three test diets containing casein + r-chagasin (0.25, 0.5 and 1% of total protein) were offered to rats (10 days). The test diets did not show adverse effects upon the development, organ weight, hematological parameters and serum protein profiles of rats, providing preliminary information on the safety of r-chagasin.
authors.affiliation: Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, Brasil.
authors.forename: Osmundo B
authors.initials: OB
authors.lastname: Oliveira Neto
authors.forename: Davi F
authors.initials: DF
authors.lastname: Farias
authors.forename: Ilka M
authors.initials: IM
authors.lastname: Vasconcelos
authors.forename: Norma S
authors.initials: NS
authors.lastname: Paes
authors.forename: Ana C S
authors.initials: AC
authors.lastname: Monteiro
authors.forename: Maria C M da
authors.initials: MC
authors.lastname: Silva
authors.forename: Luciane M
authors.initials: LM
authors.lastname: Guimarães
authors.forename: Ana F U
authors.initials: AF
authors.lastname: Carvalho
authors.forename: Maria F
authors.initials: MF
authors.lastname: Grossi-de-Sá
mesh_terms: D000821:Animal Feed / Q000633:toxicity*; D000818:Animals; D019476:Insect Proteins / Q000008:administration & dosage / Q000633:toxicity*; D008297:Male; D023421:Models, Animal; D009929:Organ Size / Q000187:drug effects*; D010572:Pest Control, Biological; D051381:Rats; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000633:toxicity; D018675:Toxicity Tests / Q000379:methods; D015430:Weight Gain
chemical_list: D019476:Insect Proteins; D011994:Recombinant Proteins