title: The effects of taurine on permethrininduced cytogenetic and oxidative damage in cultured human lymphocytes.
abstract: Permethrin (PM) is a common pyrethroid pesticide used to control pests in agriculture, forestry, horticulture, health care, homes, and textile industry. It is confirmed as a strong mutagen in animals and humans. Taurine (TA) is an amino acid found in mammalian tissues that protects the cell against DNA damage. In this study, we investigated whether supplementation of human lymphocyte cultures with TA (in the concentrations of 25 μg mL-1, 50 μg mL-1 and 100 μg mL-1) provided any protection against PM toxicity applied in the concentration of 200 μg mL-1. Genotoxicity was assessed using the micronucleus (MN) and sister chromatid exchanges (SCE) tests. In addition, we measured the total antioxidant capacity (TAC) and total oxidative stress (TOS) levels in the plasma to determine oxidative effects. PM increased SCE and MN levels and altered TAC and TOS levels. TA alone did not affect SCE and MN levels compared to controls, regardless of the concentration applied. In addition, it increased TAC levels without changing TOS levels. Moreover, it significantly buffered the negative cytogenetic and oxidative effects induced by PM in a clear dose-dependent manner. In conclusion, this study is the first to evidence the beneficial effects of TA against PM-induced DNA and oxidative damages in vitro.
authors.affiliation: Department of Molecular Biology and Genetics, Erzurum Technical University, Erzurum, Turkey.
authors.forename: Hasan
authors.initials: H
authors.lastname: Turkez
authors.forename: Elanur
authors.initials: E
authors.lastname: Aydin
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007306:Insecticides / Q000633:toxicity*; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D015162:Micronucleus Tests; D009152:Mutagenicity Tests; D018384:Oxidative Stress / Q000187:drug effects*; D026023:Permethrin / Q000633:toxicity*; D012854:Sister Chromatid Exchange / Q000187:drug effects*; D013654:Taurine / Q000494:pharmacology*; D055815:Young Adult
chemical_list: D007306:Insecticides; D013654:Taurine; D026023:Permethrin