title: Shewanella                      seohaensis sp. nov., isolated from a tidal flat sediment.
abstract: A Gram-negative, motile and rod-shaped bacterial strain, designated S7-3(T), was isolated from a tidal flat sediment at Saemankum on the western coast of Korea. Phylogenetic analyses based on 16S rRNA gene and gyrB sequences showed that strain S7-3(T) belonged to the genus Shewanella, clustering with Shewanella decolorationis S12(T). Strain S7-3(T) exhibited 98.8 % 16S rRNA gene sequence similarity and 96.8 % gyrB sequence similarity to S. decolorationis S12(T), respectively. The 16S rRNA gene sequence similarity values between strain S7-3(T) and other members of the genus Shewanella were in the range of 93.0-98.0 %. Strain S7-3(T) contained simultaneously both menaquinones (MK) and ubiquinones (Q); the predominant menaquinone was MK-7 and the predominant ubiquinones were Q-7 and Q-8. The fatty acid profiles of strain S7-3(T) and S. decolorationis JCM 21555(T) were similar; major components were C(17:1)                         ω8c, iso-C(15:0) and iso-C(15:0) 2-OH and/or C(16:1)                         ω7c. The DNA G+C content of strain S7-3(T) was 51.8 mol% and its mean DNA-DNA relatedness value with S. decolorationis JCM 21555(T) was 43 %. Differential phenotypic properties of strain S7-3(T), together with the phylogenetic and genetic distinctiveness, revealed that this strain is distinguishable from recognized Shewanella species. On the basis of the data presented, strain S7-3(T) is considered to represent a novel Shewanella species, for which the name Shewanella seohaensis sp. nov. is proposed. The type strain is S7-3(T) (=KCTC 23556(T) = CCUG 60900(T)).
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea. jhyoon69@skku.edu
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Yoon
authors.forename: Sooyeon
authors.initials: S
authors.lastname: Park
authors.forename: Yong-Taek
authors.initials: YT
authors.lastname: Jung
authors.forename: Jung-Sook
authors.initials: JS
authors.lastname: Lee
mesh_terms: D001482:Base Composition; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D027081:DNA Gyrase / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D020592:Shewanella / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D014451:Ubiquinone / Q000032:analysis; D024482:Vitamin K 2 / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D014451:Ubiquinone; D027081:DNA Gyrase