title: [Neutrophil dependent breakdown of biofilms formed by Staphylococcus aureus].
abstract: OBJECTIVE
  Study of effects during interaction of neutrophils with Staphylococcus aureus biofilms in vitro.
  
  
  METHODS
  2 day biofilms (BF) based on Staphylococcus aureus (strain 5983) were used as a model. Destruction of BF was evaluated at 5, 15, 30, 45 and 60 minutes of incubation with neutrophils. Quantitative evaluation of staphylococci was performed by using microfilming (Shangrao Optical Instruments) and a computer program described by I.V.Chebotaret al., 2010. Measurement of thickness of BF during reaction with neutrophils was evaluated by using laser scanning microscope LSM 510 Meta (Zeiss).
  
  
  RESULTS
  Human neutrophils can influence the breakdown effect on BF formed by S. auerus. A pronounced breakdown was observed at 15 minutes of the reaction, by 45 minutes staphylococci content in the BF remained only around 2%. Breakdown of BF was accompanied by an increase of quantity of viable staphylococci in the supernatant. Around 80% of neutrophils perished at 30 minutes of interaction with BE Neutrophil lysate at 30 minutes caused 30% destruction of staphylococci BF.
  
  
  CONCLUSIONS
  Adhesive staphylococci are more resistant to the effect of neutrophils than BE Neutrophil dependent destruction of staphylococci BF could be the result ofbreakdown of intracellular matrix by phagocyte secretion products and direct phagocytosis.
authors.forename: I V
authors.initials: IV
authors.lastname: Chebotar'
authors.forename: E D
authors.initials: ED
authors.lastname: Konchakova
authors.forename: N I
authors.initials: NI
authors.lastname: Evteeva
mesh_terms: D001422:Bacterial Adhesion; D058491:Bacterial Load; D018441:Biofilms / Q000254:growth & development*; D018929:Cell Culture Techniques; D002470:Cell Survival / Q000276:immunology; D005404:Fixatives; D006801:Humans; D007958:Leukocyte Count; D050296:Microbial Viability / Q000276:immunology; D009504:Neutrophils / Q000166:cytology / Q000276:immunology*; D010587:Phagocytosis / Q000276:immunology*; D013194:Staining and Labeling; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000254:growth & development* / Q000276:immunology
chemical_list: D005404:Fixatives