title: Stromal vascular fraction isolated from lipo-aspirates using an automated processing system: bench and bed analysis.
abstract: The heterogeneous stromal vascular fraction (SVF), containing adipose-derived stem/progenitor cells (ASCs), can be easily isolated through enzymatic digestion of aspirated adipose tissue. In clinical settings, however, strict control of technical procedures according to standard operating procedures and validation of cell-processing conditions are required. Therefore, we evaluated the efficiency and reliability of an automated system for SVF isolation from adipose tissue. SVF cells, freshly isolated using the automated procedure, showed comparable number and viability to those from manual isolation. Flow cytometric analysis confirmed an SVF cell composition profile similar to that after manual isolation. In addition, the ASC yield after 1 week in culture was also not significantly different between the two groups. Our clinical study, in which SVF cells isolated with the automated system were transplanted with aspirated fat tissue for soft tissue augmentation/reconstruction in 42 patients, showed satisfactory outcomes with no serious side-effects. Taken together, our results suggested that the automated isolation system is as reliable a method as manual isolation and may also be useful in clinical settings. Automated isolation is expected to enable cell-based clinical trials in small facilities with an aseptic room, without the necessity of a good manufacturing practice-level cell processing area.
authors.affiliation: Department of Plastic Surgery, University of Tokyo School of Medicine, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Doi
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Tanaka
authors.forename: Hideo
authors.initials: H
authors.lastname: Iida
authors.forename: Hitomi
authors.initials: H
authors.lastname: Eto
authors.forename: Harunosuke
authors.initials: H
authors.lastname: Kato
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Aoi
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Kuno
authors.forename: Toshitsugu
authors.initials: T
authors.lastname: Hirohi
authors.forename: Kotaro
authors.initials: K
authors.lastname: Yoshimura
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D000368:Aged; D001331:Automation / Q000379:methods*; D002452:Cell Count; D002467:Cell Nucleus / Q000378:metabolism; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D002478:Cells, Cultured; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D015187:Lipectomy; D008297:Male; D008875:Middle Aged; D017154:Stromal Cells / Q000166:cytology; D013347:Subcellular Fractions / Q000378:metabolism; D055815:Young Adult