title: Reduction of lesion in injured rat spinal cord and partial functional recovery of motility after bone marrow derived mesenchymal stem cell transplantation.
abstract: OBJECTIVE
  This study aimed to analyze the effect of rat bone marrow-mesenchymal stem     cells (rBM-MSCs) delivery on lesion site after spinal cord injury, and to     observe the functional recovery after transplantation.
  
  
  METHODS
  MSCs were isolated from rat femurs and tibias. The experimental rat population     was divided into four groups: only laminectomy (1); laminectomy+trauma (2);     laminectomy+trauma+PBS (3); laminectomy+trauma+MSCs (4). Their motility were     scored regularly. After 4-weeks, rats were sacrificed, and their spinal cords     were examined for GFP labeled rBM-MSCs by immunostainings.
  
  
  RESULTS
  In the early     posttraumatic period, the ultrastructures of spinal cord tissue were preserved     in Group 4. The majority of cells forming the ependymal region around the     central canal were found to be MSCs. The gray-and-white-matter around the     ependymal region were composed of Nestin+/GFAP+ cells, with astrocytic-like     appearance. The scores showed significant motor recovery in Group 4, especially     in hind limb functions. However, no obvious change was observed in other groups.
  
  
  CONCLUSIONS
  The increase Nestin+/GFAP+ cells in the gray-and-white-matter around     the ependymal region could indicate the potential to self-renew and plasticity.     Thus, transplantation of rBM-MSCs might be an effective strategy to improve     functional recovery following spinal cord trauma. In conclusion, molecular     factors in cell fate decisions could be manipulated to enhance reparative     potential of cell-based therapy.
authors.affiliation: Kocaeli University, Faculty of Medicine, Department of Stem Cell, Kocaeli, Turkey. ekaraoz@hotmail.com
authors.forename: Erdal
authors.initials: E
authors.lastname: Karaoz
authors.forename: Serdar
authors.initials: S
authors.lastname: Kabatas
authors.forename: Gokhan
authors.initials: G
authors.lastname: Duruksu
authors.forename: Alparslan
authors.initials: A
authors.lastname: Okcu
authors.forename: Cansu
authors.initials: C
authors.lastname: Subasi
authors.forename: Birol
authors.initials: B
authors.lastname: Ay
authors.forename: Murat
authors.initials: M
authors.lastname: Musluman
authors.forename: Erdinc
authors.initials: E
authors.lastname: Civelek
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D016026:Bone Marrow Transplantation / Q000379:methods*; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005260:Female; D049452:Green Fluorescent Proteins / Q000235:genetics; D007796:Laminectomy / Q000379:methods; D008159:Lumbar Vertebrae; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology; D009043:Motor Activity / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D020127:Recovery of Function / Q000502:physiology*; D013119:Spinal Cord Injuries / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D020858:Tissue and Organ Harvesting / Q000379:methods; D014945:Wound Healing / Q000502:physiology
chemical_list: D015415:Biomarkers; D049452:Green Fluorescent Proteins