title: Safety and immunogenicity of Brucella abortus strain RB51 vaccine in cross bred cattle calves in India.
abstract: Safety and immunogenicity of Brucella abortus RB51 vaccine has been evaluated in an organised dairy farm in India. All the cattle (r = 29) vaccinated with strain RB51 'responded' to the vaccine as demonstrated by iELISA using acetone killed strain RB51 antigen. The percentage responders at day 35, 60 and 90 post vaccination were 100%, 95% and 20%, respectively. Strain RB51 was able to elicit a good IFN-gamma response from vaccinated animals. The post-vaccination time point analysis indicated that the cumulative IFN-gamma response of whole blood from vaccinates stimulated with heat killed RB51 antigen was elicited in 80% of calves at 60 days post vaccination. Absence of strain RB51 in the secretions and excretion and lack of local or systemic reaction indicated the safety of the vaccine.
authors.affiliation: Department of Veterinary Microbiology, G B Pant University of Agriculture and Technology, Pantnagar 263 145, India. madan_rs@rediffmail.com
authors.forename: Rashmi
authors.initials: R
authors.lastname: Singh
authors.forename: Sanjay Singh
authors.initials: SS
authors.lastname: Basera
authors.forename: Kamal
authors.initials: K
authors.lastname: Tewari
authors.forename: Shweta
authors.initials: S
authors.lastname: Yadav
authors.forename: Sumit
authors.initials: S
authors.lastname: Joshi
authors.forename: Brajesh
authors.initials: B
authors.lastname: Singh
authors.forename: Falguni
authors.initials: F
authors.lastname: Mukherjee
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D002004:Brucella Vaccine / Q000009:adverse effects / Q000276:immunology / Q000627:therapeutic use*; D002003:Brucella abortus / Q000276:immunology*; D002007:Brucellosis, Bovine / Q000276:immunology / Q000517:prevention & control*; D002417:Cattle; D007194:India; D007371:Interferon-gamma / Q000097:blood / Q000276:immunology; D015047:Zoonoses
chemical_list: D000907:Antibodies, Bacterial; D002004:Brucella Vaccine; D007371:Interferon-gamma