title: Graphene oxide integrated sensor for electrochemical monitoring of mitomycin C-DNA interaction.
abstract: We present a graphene oxide (GO) integrated disposable electrochemical sensor for the enhanced detection of nucleic acids and the sensitive monitoring of the surface-confined interactions between the anticancer drug mitomycin C (MC) and DNA. Interfacial interactions between immobilized calf thymus double-stranded (dsDNA) and anticancer drug MC were investigated using differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) techniques. Based on three repetitive voltammetric measurements of 120 μg mL(-1) DNA immobilized on GO-modified electrodes, the RSD % (n = 3) was calculated as 10.47% and the detection limit (DL) for dsDNA was found to be 9.06 μg mL(-1). EIS studies revealed that the binding of the drug MC to dsDNA leads to a gradual decrease of its negative charge. As a consequence of this interaction, the negative redox species were allowed to approach the electrode, and thus increase the charge transfer kinetics. On the other hand, DPV studies exploited the decrease of the guanine signal due to drug binding as the basis for specifically probing the biointeraction process between MC and dsDNA.
authors.affiliation: Ege University, Faculty of Pharmacy, Analytical Chemistry Dept., Bornova, 35100 Izmir, Turkey. arzum.erdem@ege.edu.tr
authors.forename: Arzum
authors.initials: A
authors.lastname: Erdem
authors.forename: Mihrican
authors.initials: M
authors.lastname: Muti
authors.forename: Pagona
authors.initials: P
authors.lastname: Papakonstantinou
authors.forename: Ece
authors.initials: E
authors.lastname: Canavar
authors.forename: Hakan
authors.initials: H
authors.lastname: Karadeniz
authors.forename: Gulsah
authors.initials: G
authors.lastname: Congur
authors.forename: Surbhi
authors.initials: S
authors.lastname: Sharma
mesh_terms: D000818:Animals; D002417:Cattle; D004247:DNA / Q000378:metabolism*; D058266:Dielectric Spectroscopy; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D006108:Graphite / Q000737:chemistry*; D016685:Mitomycin / Q000378:metabolism*; D010087:Oxides / Q000737:chemistry*
chemical_list: D010087:Oxides; D016685:Mitomycin; D006108:Graphite; D004247:DNA