title: Effect of caloric intake on Western-style diet-induced intestinal tumors in a mouse model for hereditary colon cancer.
abstract: Increased caloric intake has been associated with increased risk for cancer of the large intestine. We studied caloric intake effect on tumor formation in Apc1638( N/+ ) mice, a preclinical model for human familial adenomatous polyposis. Mice were fed a controlled AIN-76A diet or a new Western-style diet (NWD). Intestinal tumor development was evaluated after 6 mo of feeding 1) AIN-76A diet (fed ad libitum) vs. AIN-76A (caloric intake reduced 30%); 2) NWD (fed ad libitum) vs. NWD (caloric intake reduced 30%); and 3) AIN-76A (fed ad libitum) vs. NWD (paired-fed with NWD providing equal caloric intakes to AIN-76A). Intestinal tumor incidences were 78-100% with intergroup variation P > 0.05; however, tumor multiplicity responded differently to dietary treatment: 1) Tumor multiplicity was unchanged after AIN-76A (caloric intake reduced 30% vs. mice fed AIN-76A ad libitum); 2) tumor multiplicity was unchanged after NWD (caloric intake reduced 30% vs. NWD ad libitum); and 3) tumor multiplicity increased 130% after NWD was paired-fed with the same caloric intake as mice fed AIN-76A ad libitum (P < 0.05). Body weights showed no association with tumor development. Findings indicated modified nutrients in NWD were mainly responsible for increased tumors in mice fed NWD vs. AIN-76A in this preclinical mouse model for human FAP.
authors.affiliation: Department of Medicine (Gastroenterology and Hepatology), Weill Cornell Medical College, New York, NY 10021, USA.
authors.forename: Osamu
authors.initials: O
authors.lastname: Itano
authors.forename: Kunhua
authors.initials: K
authors.lastname: Fan
authors.forename: Kan
authors.initials: K
authors.lastname: Yang
authors.forename: Keiich
authors.initials: K
authors.lastname: Suzuki
authors.forename: Fred
authors.initials: F
authors.lastname: Quimby
authors.forename: Zhiqian
authors.initials: Z
authors.lastname: Dong
authors.forename: Bo
authors.initials: B
authors.lastname: Jin
authors.forename: Winfried
authors.initials: W
authors.lastname: Edelmann
authors.forename: Martin
authors.initials: M
authors.lastname: Lipkin
mesh_terms: D011125:Adenomatous Polyposis Coli / Q000473:pathology; D000818:Animals; D017209:Apoptosis; D001835:Body Weight; D002471:Cell Transformation, Neoplastic; D003106:Colon / Q000473:pathology; D003110:Colonic Neoplasms / Q000473:pathology*; D004032:Diet; D004195:Disease Models, Animal; D002149:Energy Intake; D005260:Female; D008297:Male; D051379:Mice; D018345:Mice, Knockout