title: Cellular toxicity of isoniazid together with rifampicin and the metabolites of isoniazid on QSG-7701 hepatocytes.
abstract: OBJECTIVE
  To investigate the cellular toxicity of isoniazid together with rifampicin and the metabolites of isoniazid on cultured QSG-7701 cells lines.
  
  
  METHODS
  Isoniazid, rifampicin, mixture of rifampicin and isoniazid, acetylhydrazine, hydrazine were added in cultural media of QSG-7701 cells and cultured for 48 hours. The survival rate of cells was determined by MTT method. The cultural media and cells were collected and the activity of lactate dehydrogenase was detected by chromatometry.
  
  
  RESULTS
  Compared with control group, the survival rate decreased significantly and the lactate dehydrogenase released from cell increased significantly in cells treated with isoniazid, rifampicin, acetylhydrazine, hydrazine. Hydrazine, the metabolite of isoniazid produced significant damage on hepatocytes in low concentration.
  
  
  CONCLUSIONS
  Rifampicin together with rifampicin and metabolites of isoniazid produce cellular toxic effects and hydrazine may be the most toxiferous metabolite.
authors.affiliation: Department of Respiratory Medicine, First Affiliated Hospital of Hebei Northern College, Zhangjiakou 075000, Hebei, China. zzh80018@yahoo.com.cn
authors.forename: Zhi-Hua
authors.initials: ZH
authors.lastname: Zhang
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Tang
authors.forename: Zhi-Lin
authors.initials: ZL
authors.lastname: Zhan
authors.forename: Xiu-Long
authors.initials: XL
authors.lastname: Zhang
authors.forename: Hong-Hai
authors.initials: HH
authors.lastname: Wu
authors.forename: Yan-Ning
authors.initials: YN
authors.lastname: Hou
mesh_terms: D000704:Analysis of Variance; D000995:Antitubercular Agents / Q000633:toxicity*; D016022:Case-Control Studies; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology*; D004338:Drug Combinations; D022781:Hepatocytes / Q000187:drug effects*; D006801:Humans; D007538:Isoniazid / Q000633:toxicity*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D012293:Rifampin / Q000633:toxicity*
chemical_list: D000995:Antitubercular Agents; D004338:Drug Combinations; D007770:L-Lactate Dehydrogenase; D007538:Isoniazid; D012293:Rifampin