title: Streptococcus troglodytae sp. nov., from the chimpanzee oral cavity.
abstract: Six strains, TKU 25, TKU 28, TKU 30, TKU 31(T), TKU 33 and TKU 34, were isolated from the oral cavity of a chimpanzee (Pan troglodytes). Colonies of strains grown on Mitis-Salivarius agar were similar in morphology to that of Streptococcus mutans. The novel strains were Gram-stain-positive, facultatively anaerobic cocci that lacked catalase activity. Analysis of the partial 16S rRNA gene sequences of these isolates showed that the most closely related strain was the type strain of S. mutans (96.4 %). The next closely related strains to the isolates were the type strains of Streptococcus devriesei (94.5 %) and Streptococcus downei (93.9 %). These isolates could be distinguished from S. mutans by inulin fermentation and alkaline phosphatase activity (API ZYM system). The peptidoglycan type of the novel isolates was Glu-Lys-Ala(3). Strains were not susceptible to bacitracin. On the basis of phenotypic characterization, partial 16S rRNA gene and two housekeeping gene (groEL and sodA) sequence data, we propose a novel taxon, Streptococcus troglodytae sp. nov.; the type strain is TKU 31(T) ( = JCM 18038(T) = DSM 25324(T)).
authors.affiliation: Department of Oral Microbiology, Tsurumi University School of Dental Medicine, 2-1-3, Tsurumi, Tsurumi-ku, Yokohama 230-8501, Japan.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Okamoto
authors.affiliation: Department of Translational Research, Tsurumi University School of Dental Medicine, 2-1-3, Tsurumi, Tsurumi-ku, Yokohama 230-8501, Japan.
authors.forename: Susumu
authors.initials: S
authors.lastname: Imai
authors.affiliation: Department of Translational Research, Tsurumi University School of Dental Medicine, 2-1-3, Tsurumi, Tsurumi-ku, Yokohama 230-8501, Japan.
authors.forename: Mayu
authors.initials: M
authors.lastname: Miyanohara
authors.affiliation: Department of Operative Dentistry, Tsurumi University School of Dental Medicine, 2-1-3, Tsurumi, Tsurumi-ku, Yokohama 230-8501, Japan.
authors.forename: Wataru
authors.initials: W
authors.lastname: Saito
authors.affiliation: Department of Operative Dentistry, Tsurumi University School of Dental Medicine, 2-1-3, Tsurumi, Tsurumi-ku, Yokohama 230-8501, Japan.
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Momoi
authors.affiliation: Department of Translational Research, Tsurumi University School of Dental Medicine, 2-1-3, Tsurumi, Tsurumi-ku, Yokohama 230-8501, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Abo
authors.affiliation: Department of Translational Research, Tsurumi University School of Dental Medicine, 2-1-3, Tsurumi, Tsurumi-ku, Yokohama 230-8501, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Nomura
authors.affiliation: Department of Fixed Prosthodontics, Tsurumi University School of Dental Medicine, 2-1-3, Tsurumi, Tsurumi-ku, Yokohama 230-8501, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Ikawa
authors.affiliation: Department of Fixed Prosthodontics, Tsurumi University School of Dental Medicine, 2-1-3, Tsurumi, Tsurumi-ku, Yokohama 230-8501, Japan.
authors.forename: Takumi
authors.initials: T
authors.lastname: Ogawa
authors.affiliation: Center for Human Evolution Modeling Research, Primate Research Institute, Kyoto University, Inuyama, Aichi 484-8506, Japan.
authors.forename: Takako
authors.initials: T
authors.lastname: Miyabe-Nishiwaki
authors.affiliation: Center for Human Evolution Modeling Research, Primate Research Institute, Kyoto University, Inuyama, Aichi 484-8506, Japan.
authors.forename: Akihisa
authors.initials: A
authors.lastname: Kaneko
authors.affiliation: Center for Human Evolution Modeling Research, Primate Research Institute, Kyoto University, Inuyama, Aichi 484-8506, Japan.
authors.forename: Akino
authors.initials: A
authors.lastname: Watanabe
authors.affiliation: Center for Human Evolution Modeling Research, Primate Research Institute, Kyoto University, Inuyama, Aichi 484-8506, Japan.
authors.forename: Shohei
authors.initials: S
authors.lastname: Watanabe
authors.affiliation: Language and Intelligence Section, Primate Research Institute, Kyoto University, Inuyama, Aichi 484-8506, Japan.
authors.forename: Misato
authors.initials: M
authors.lastname: Hayashi
authors.affiliation: Language and Intelligence Section, Primate Research Institute, Kyoto University, Inuyama, Aichi 484-8506, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Tomonaga
authors.affiliation: Department of Translational Research, Tsurumi University School of Dental Medicine, 2-1-3, Tsurumi, Tsurumi-ku, Yokohama 230-8501, Japan.
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Hanada
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D007444:Inulin / Q000378:metabolism; D008969:Molecular Sequence Data; D009055:Mouth / Q000382:microbiology*; D002679:Pan troglodytes / Q000382:microbiology*; D010457:Peptidoglycan / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013291:Streptococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D007444:Inulin; D000469:Alkaline Phosphatase