title: Histopathological analysis of corticosteroid-antibiotic preparation and propolis paste formulation as intracanal medication after pulpectomy: an in vivo study.
abstract: Intracanal medication in pulpectomy therapy is used between appointments with the objective of reducing pain and inflammatory processes in pulp and periapical tissues. Propolis has been known as a natural antibiotic and has been subject of medical and dental research due to its therapeutic properties such as antibiotic, analgesic and anti-inflammatory effects.
  
  
  OBJECTIVE
  The aim was to carry out an in vivo evaluation of the periapical tissue response to propolis paste when used as an intracanal medication in the teeth of dogs after pulpectomy.
  
  
  METHODS
  72 dog's incisors were selected for the experiment. After biomechanical preparation the root canal was filled with a corticosteroid-antibiotic preparation, experimental propolis paste, non-medicament (negative control) or non-pulpectomy at all (positive control). The medications were left inside the root canal for 7, 14 or 28 days. At the end of the experimental period histological sections were prepared and all laboratories processes for Harris hematoxylin and eosin staining was proceeded followed by the analysis using an optical microscope. Sections were classified according to a score representing the inflammatory events observed: the presence of polymorphonuclear neutrophils, polymorphonuclear eosinophils, lymphocytes and plasma cells, macrophages and/or giant cells, fibrous condensation and abscesses.
  
  
  RESULTS
  There were statistically significant differences between the tissue reactions caused by the two substances being tested, after different experimental periods, with the periapical tissue that was in contact with propolis paste exhibiting fewer inflammatory reactions in comparison to corticosteroid-antibiotic preparation.
  
  
  CONCLUSIONS
  The low tissue responses from propolis paste suggest that this material could be considered as an option for root canal medication after pulpectomy.
authors.affiliation: Department of Biology and Health Science, University of Planalto Catarinense, SC, Brazil.
authors.forename: Isabela França de Almeida Santos
authors.initials: IF
authors.lastname: Ramos
authors.forename: Michelle Tillmann
authors.initials: MT
authors.lastname: Biz
authors.forename: Niraldo
authors.initials: N
authors.lastname: Paulino
authors.forename: Amarilis
authors.initials: A
authors.lastname: Scremin
authors.forename: Alvaro
authors.initials: A
authors.lastname: Della Bona
authors.forename: Fernando Branco
authors.initials: FB
authors.lastname: Barletta
authors.forename: José Antônio Poli de
authors.initials: JA
authors.lastname: Figueiredo
mesh_terms: D000305:Adrenal Cortex Hormones / Q000494:pharmacology*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003782:Dental Pulp / Q000187:drug effects; D003786:Dental Pulp Cavity / Q000187:drug effects; D004285:Dogs; D023421:Models, Animal; D010486:Periapical Tissue / Q000187:drug effects*; D011429:Propolis / Q000494:pharmacology*; D011670:Pulpectomy; D012388:Root Canal Irrigants / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D000305:Adrenal Cortex Hormones; D000900:Anti-Bacterial Agents; D012388:Root Canal Irrigants; D011429:Propolis