title: Effects of dietary molybdenum, sulfur and zinc on the excretion and tissue accumulation of trace elements in sheep fed palm kernel cake-based diets.
abstract: Twelve male 8-month-old lambs were used in a 6-month feeding experiment to determine the effects of dietary Mo, Mo + S and Zn supplements on the body retention and tissue accumulation of dietary Cu, Zn and Fe. The lambs were divided into four groups of three lambs each and each group was fed ad libitum one of four diets. A control diet was based on palm kernel cake (PKC) and grass hay. Three additional diets were the control supplemented with either Mo or Mo+S or Zn. At 3 months of the experiment, feces and urine were collected and sampled for 6 days. At the end of the experiment (6 months), blood was sampled and then the sheep were slaughtered. The liver and kidney were removed and sampled for chemical analysis. In comparison with the control, each dietary supplement decreased (P<0.05) the Cu concentration in the liver, but only the Mo+S supplement decreased it to a safe range of below 350 μg/g dry matter. This was accompanied by the body retention of dietary Cu of 24.6%, 6.7%, 2.5% and 6.5% for the control, Mo, Mo+S and Zn treatments, respectively. The blood plasma concentration of Cu was decreased (P<0.05) by the Zn supplement, but was not affected by other supplements (P>0.05). It was concluded that from the supplements tested, only Mo+S appeared to be effective in reducing the retention and liver accumulation of the dietary Cu to prevent chronic Cu toxicity in sheep fed PKC-based diets.
authors.affiliation: Institute of Tropical Agriculture, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: R A
authors.initials: RA
authors.lastname: Al-Kirshi
authors.forename: A R
authors.initials: AR
authors.lastname: Alimon
authors.forename: M
authors.initials: M
authors.lastname: Ivan