title: Association between EGF +61 genetic polymorphisms and non-small cell lung cancer increased risk in a Portuguese population: a case-control study.
abstract: Epidermal growth factor (EGF) and its receptor play critical roles in non-small cell lung cancer (NSCLC) carcinogenesis. A functional polymorphism in the EGF gene has been linked to increased cancer susceptibility. This study aimed to evaluate the role of the EGF +61A/G polymorphism as risk factors in NSCLC patients. For the present case-control study, we analyzed 112 NSCLC and 126 cancer-free controls from Portugal. Following DNA isolation from peripheral blood, EGF +61A/G polymorphism was assessed by polymerase chain reaction-restriction fragment length polymorphism. Univariate and multivariate logistic regression analyses were used to calculate odds ratio (OR) and 95 % confidence intervals (95 % CI). False-positive report probability was also assessed. The EGF +61 genotypes frequencies in NSCLC were AA (23.2 %), AG (51.8 %), and GG (25 %) and in controls, AA (40.5 %), AG (41.3 %), and GG (18.3 %). When compared to the reference genotype (EGF +61A/A), we found a statistically significant association between EGF +61 A/G (OR = 2.142, 95 % CI 1.170-3.924) and EGF +61G/G (OR = 2.398, 95 % CI 1.157-4.968) genotypes and susceptibility to development of NSCLC. Furthermore, stratification by sex revealed a trend to increased risk of males carrying +61A/G genotype for developing NSCLC (OR = 2.044, 95 % CI 0.998-4.188) when compared to A/A genotype. Our data suggest an increased risk to develop NSCLC in Portuguese population carrying the EGF +61A/G and +61G/G genotypes.
authors.affiliation: Department of Medicine, São João Hospital, Faculty of Medicine, University of Porto, Alameda Prof. Hernani Monteiro, 4200-319, Porto, Portugal. ramondemello@gmail.com
authors.forename: Ramon Andrade
authors.initials: RA
authors.lastname: de Mello
authors.forename: Mónica
authors.initials: M
authors.lastname: Ferreira
authors.forename: Sandra
authors.initials: S
authors.lastname: Costa
authors.forename: Bruno Marques
authors.initials: BM
authors.lastname: Costa
authors.forename: Filipa Soares
authors.initials: FS
authors.lastname: Pires
authors.forename: Inês
authors.initials: I
authors.lastname: Neves
authors.forename: Maria Inês
authors.initials: MI
authors.lastname: Almeida
authors.forename: João
authors.initials: J
authors.lastname: Cunha
authors.forename: Pedro
authors.initials: P
authors.lastname: Oliveira
authors.forename: Venceslau
authors.initials: V
authors.lastname: Hespanhol
authors.forename: Rui Manuel
authors.initials: RM
authors.lastname: Reis
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D004815:Epidermal Growth Factor / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D020641:Polymorphism, Single Nucleotide; D011174:Portugal; D015203:Reproducibility of Results; D012306:Risk
chemical_list: D004815:Epidermal Growth Factor