title: Exploration of diuretic potential and electrolyte excretion of Tephrosia purpurea (Fabaceae) in rats.
abstract: Tephrosia purpurea (Fabaceae) is a well-known traditional plant with diuretic effect but no scientific work published till date to support the claimed ethnomedical use. Therefore, the present study appraised the diuretic potential of methanol extract of Tephrosia purpurea (METP) in male wistar rats. The powdered plant material was extracted with methanol by hot extraction. The animals were divided into five groups for diuretic activity. The first group served as saline control (0.9%% saline solution, 25 ml/kg, body weight (b.w)), the second group received osmotic diuretic, urea (1 g/kg b.w), the third group received high-ceiling diuretic, furosemide (5 mg/kg b.w), and the other two groups were administered various concentrations of METP (200 mg/kg and 400 mg/kg b.w) orally to hydrated rats and their urine volume was measured at 5th and 24th hr after drug administration, while animals were deprived of food and water. After collection of urine, the parameters such as urine output, diuretic activity, electrolyte excretion of Na(++), K(++), Ca(2++), and Cl(-), and pH were analyzed. METP at various dose levels exhibited significant diuretic activity as evidenced by increased urine volume, electrolyte concentration, and alkaline pH in comparison to control group of animals. The present study provides a quantitative basis for explaining the folkloric use of Tephrosia purpurea as a diuretic agent in Indian traditional system of medicine.
authors.affiliation: Department of Pharmacognosy, Dr HLT College of Pharmacy, Channaaptna, Karnataka, India. ashokkumardurairaj@yahoo.co.in
authors.forename: D
authors.initials: D
authors.lastname: Ashokkumar
authors.forename: T V
authors.initials: TV
authors.lastname: Narayana
authors.lastname: Vidyasagar
authors.forename: Upal Kanti
authors.initials: UK
authors.lastname: Mazumder
authors.forename: Malaya
authors.initials: M
authors.lastname: Gupta
mesh_terms: D000818:Animals; D004231:Diuresis / Q000187:drug effects*; D004232:Diuretics / Q000494:pharmacology*; D004573:Electrolytes / Q000652:urine*; D005665:Furosemide / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008297:Male; D008515:Medicine, Ayurvedic; D028321:Plant Preparations / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D031304:Tephrosia; D014508:Urea / Q000494:pharmacology; D014554:Urination / Q000187:drug effects*
chemical_list: D004232:Diuretics; D004573:Electrolytes; D028321:Plant Preparations; D005665:Furosemide; D014508:Urea