title: Cloning, purification, crystallization and preliminary X-ray analysis of the Burkholderia pseudomallei L1 ribosomal protein.
abstract: The gene encoding the L1 ribosomal protein from Burkholderia pseudomallei strain D286 has been cloned into the pETBLUE-1 vector system, overexpressed in Escherichia coli and purified. Crystals of the native protein were grown by the hanging-drop vapour-diffusion technique using PEG 3350 as a precipitant and diffracted to beyond 1.65 Å resolution. The crystals belonged to space group P2(1)2(1)2, with unit-cell parameters a = 53.6, b = 127.1, c = 31.8 Å and with a single molecule in the asymmetric unit.
authors.affiliation: Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, England.
authors.forename: Abd Ghani
authors.initials: AG
authors.lastname: Abd Aziz
authors.forename: Sergey N
authors.initials: SN
authors.lastname: Ruzheinikov
authors.forename: Svetlana E
authors.initials: SE
authors.lastname: Sedelnikova
authors.forename: Rahmah
authors.initials: R
authors.lastname: Mohamed
authors.forename: Sheila
authors.initials: S
authors.lastname: Nathan
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Baker
authors.forename: David W
authors.initials: DW
authors.lastname: Rice
mesh_terms: D016957:Burkholderia pseudomallei / Q000737:chemistry*; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D012269:Ribosomal Proteins / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D012269:Ribosomal Proteins; C028546:ribosomal protein L1