title: [Hsa-miR-654-5p regulates osteogenic differentiation of human bone marrow mesenchymal stem cells by repressing bone morphogenetic protein 2].
abstract: OBJECTIVE
  To study the effect of hsa-miR-654-5p in repressing bone morphogenetic protein 2 (BMP2) mRNA and protein in human bone marrow mesenchymal stem cells (hBMSCs), and explore its regulatory role in osteogenic differentiation of hBMSCs.
  
  
  METHODS
  hBMSCs in the 4th passage were cultured for 16 h and transfected with hsa-miR-654-5p followed by further culture for 48 h. qRT-PCR and Western blotting were performed to detect the expressions of BMP2 mRNA and protein. Dual-luciferase?reporter gene assay was employed to examine the repression of the BMP2 gene.
  
  
  RESULTS
  BMP2 mRNA and protein expressions were significantly down-regulated in hBMSCs with hsa-miR-654-5p overexpression. Dualluci-ferase reporter gene assay indicated that the predicted target site of BMP2 was repressed directly by hsa-miR-654-5p, but this repression did not occur at the mutant predicted target site of BMP2.
  
  
  CONCLUSIONS
  hsa-miR-654-5p can directly repress the mRNA and protein expressions of BMP2 by binding to a specific target site. The changes in hsa-miR-654-5p can play an important role in osteogenic differentiation regulation of hBMSCs.
authors.affiliation: Department of Orthopedics, General Hospital of PLA, Beijing 100853, China. weijunqiang301@126.com
authors.forename: Jun-Qiang
authors.initials: JQ
authors.lastname: Wei
authors.forename: Hua
authors.initials: H
authors.lastname: Chen
authors.forename: Xiao-Fei
authors.initials: XF
authors.lastname: Zheng
authors.forename: Bo-Xun
authors.initials: BX
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Pei-Fu
authors.initials: PF
authors.lastname: Tang
authors.forename: Fei
authors.initials: F
authors.lastname: She
authors.forename: Qing
authors.initials: Q
authors.lastname: Song
authors.forename: Tan-Shi
authors.initials: TS
authors.lastname: Li
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology; D055396:Bone Morphogenetic Protein 2 / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D010006:Osteoblasts / Q000166:cytology; D010012:Osteogenesis / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014162:Transfection
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D035683:MicroRNAs; D012333:RNA, Messenger