title: Antiproliferative and antiangiogenic properties of horse chestnut extract.
abstract: This study was designed to examine the in vitro antiproliferative effect of the horse chestnut extract (HCE) on cancer cell lines. Furthermore, we have investigated the in vitro effect of HCE on some angiogenic events by using human umbilical vein endothelial cells. The cell proliferation was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and anchorage-independent growth by colony-forming assay. To understand the growth inhibitory effects, carcinoma cell lines (Jurkat, CEM, HeLa, and MCF-7) were treated with various concentrations of HCE. Incubation of Jurkat, CEM, HeLa, and MCF-7 cancer cells with HCE at 125 µg/mL for 72 h caused 93.7%, 32.3%, 20.4% and 40.4% reduction in cell survival. Colony-forming assay also confirmed growth-inhibitory effects of the compound studied. In HeLa HCE-treated cells, we found a significant increase in cells having sub-G(0) /G(1) DNA content which is considered to be a marker of apoptotic cell death. Apoptosis was also further confirmed by DNA fragmentation analysis.Furthermore, HCE inhibited migration of human umbilical vein endothelial cells as well as decreased secretion of matrix metalloproteinase and vascular endothelial growth factor.In conclusion, the present study has assessed the in vitro antiproliferative/antiangiogenic potential of HCE. These results generate a rationale for in vivo efficacy studies with horse chestnut in preclinical cancer models.
authors.affiliation: Department of Experimental Medicine, University of P. J. Šafarik, Košice, Slovak Republic. gabriela.mojzisova@upjs.sk
authors.forename: Gabriela
authors.initials: G
authors.lastname: Mojžišová
authors.forename: Ján
authors.initials: J
authors.lastname: Mojžiš
authors.forename: Martina
authors.initials: M
authors.lastname: Pilátová
authors.forename: Lenka
authors.initials: L
authors.lastname: Varinská
authors.forename: Lenka
authors.initials: L
authors.lastname: Ivanová
authors.forename: Ladislav
authors.initials: L
authors.lastname: Strojný
authors.forename: Ján
authors.initials: J
authors.lastname: Richnavský
mesh_terms: D031319:Aesculus / Q000737:chemistry*; D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D053938:DNA Fragmentation / Q000187:drug effects; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects; D006801:Humans; D010936:Plant Extracts / Q000494:pharmacology*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D020533:Angiogenesis Inhibitors; D010936:Plant Extracts; D042461:Vascular Endothelial Growth Factor A