title: The effect of conglutinin on production of reactive oxygen species in bovine granulocytes.
abstract: Conglutinin is a high molecular-weight lectin originally detected in bovine serum. It belongs to the family of collectins that bind sugar residues in a Ca(2+)-dependent manner and are effector molecules in innate immunity. Conglutinin appears to play an important role in immune defense mechanisms, showing antiviral and antibacterial activities when tested in vivo and in vitro. The present study evaluated the effect of conglutinin on the respiratory bursts in bovine peripheral phagocytes. Using nitroblue tetrazolium and hydrogen peroxide assays, we showed that sugar ligand-bound conglutinin stimulated the production of superoxide and H2O2 in granulocytes whereas the non-sugar-bound form of conglutinin inhibited these processes. These results indicate that both forms of conglutinin are able to interact with surface leukocyte receptors but have opposite effects on phagocytic activity. Our findings suggest that conglutinin bound to sugar residues on microbial surfaces can induce oxygen burst in phagocytes, and thereby mediates the elimination of pathogens and prevents the spread of infection.
authors.affiliation: Institute of Biological Bases of Animal Diseases, Sub-Department of Veterinary Prevention, Faculty of Veterinary Medicine, University of Life Sciences in Lublin. Akademicka 12, 20-033 Lublin, Poland. marta.dec@up.lublin.pl
authors.forename: Marta
authors.initials: M
authors.lastname: Dec
authors.forename: Andrzej
authors.initials: A
authors.lastname: Wernicki
authors.forename: Andrzej
authors.initials: A
authors.lastname: Puchalski
authors.forename: Renata
authors.initials: R
authors.lastname: Urban-Chmiel
authors.forename: Adam
authors.initials: A
authors.lastname: Waśko
mesh_terms: D000818:Animals; D002417:Cattle / Q000276:immunology*; D037222:Collectins / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D006098:Granulocytes / Q000187:drug effects* / Q000276:immunology; D006861:Hydrogen Peroxide / Q000276:immunology; D007113:Immunity, Innate / Q000187:drug effects / Q000276:immunology; D010587:Phagocytosis / Q000276:immunology; D017382:Reactive Oxygen Species / Q000276:immunology*; D016897:Respiratory Burst / Q000187:drug effects* / Q000276:immunology; D012712:Serum Globulins / Q000494:pharmacology*; D018709:Statistics, Nonparametric; D013481:Superoxides / Q000276:immunology
chemical_list: D037222:Collectins; D017382:Reactive Oxygen Species; D012712:Serum Globulins; C024014:conglutinin; D013481:Superoxides; D006861:Hydrogen Peroxide