title: Neolignans and sesquiterpenes from cell cultures of Stellera chamaejasme.
abstract: Two new neolignans (1, 2) and six sesquiterpenes (3- 8) were isolated from the cell cultures of Stellera chamaejasme. Their structures and absolute configurations were elucidated by extensive spectroscopic and computational methods. Compound 4 exhibited significant protective effects against CCl (4)-induced hepatotoxicity in HepG2 cells, reducing aspartate aminotransferase release by 29.49 % at 10 µM. These compounds have not been isolated from plant material, which implies that in vitro plant cell cultures may offer alternative and effective sources of bioactive natural compounds.
authors.affiliation: State Key Laboratory of Bioactive Substances and Function of Natural Medicines and Key Laboratory of Biosynthesis of Natural Products, Ministry of Health, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, PR China.
authors.forename: Li-Rui
authors.initials: LR
authors.lastname: Qiao
authors.forename: Lin
authors.initials: L
authors.lastname: Yang
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Zou
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Hua
authors.initials: H
authors.lastname: Sun
authors.forename: Yi-Kang
authors.initials: YK
authors.lastname: Si
authors.forename: Dan
authors.initials: D
authors.lastname: Zhang
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Chen
authors.forename: Jungui
authors.initials: J
authors.lastname: Dai
mesh_terms: D002478:Cells, Cultured; D056945:Hep G2 Cells; D006801:Humans; D017705:Lignans / Q000737:chemistry* / Q000302:isolation & purification*; D008968:Molecular Conformation; D010946:Plants, Medicinal / Q000737:chemistry* / Q000166:cytology; D020011:Protective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D012717:Sesquiterpenes / Q000737:chemistry* / Q000302:isolation & purification*; D029645:Thymelaeaceae / Q000737:chemistry* / Q000166:cytology*
chemical_list: D017705:Lignans; D020011:Protective Agents; D012717:Sesquiterpenes