title: Multicolour single molecule imaging on cells using a supercontinuum source.
abstract: Multicolour single molecule fluorescence imaging enables the study of multiple proteins in the membranes of living cells. We describe the use of a supercontinuum laser as the excitation source, show its comparability with multiplexed single-wavelength lasers and demonstrate that it can be used to study membrane proteins such as the ErbB receptor family. We discuss the benefits of white-light sources for single molecule fluorescence, in particular their ease of use and the freedom to use the most appropriate dye without being constrained by available laser wavelengths.
authors.forename: Stephen E D
authors.initials: SE
authors.lastname: Webb
authors.forename: Laura
authors.initials: L
authors.lastname: Zanetti-Domingues
authors.forename: Benjamin C
authors.initials: BC
authors.lastname: Coles
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Rolfe
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Wareham
authors.forename: Marisa L
authors.initials: ML
authors.lastname: Martin-Fernandez