title: Calorimetric control of the specific growth rate during fed-batch cultures of Saccharomyces cerevisiae.
abstract: The specific growth rate of a Saccharomyces cerevisiae strain with glucose as limiting C-source was estimated from the measured heat flow produced by the cells. For the cultivation a standard 30 l laboratory bioreactor was used, which was extended in such a way that heat balancing is possible. The feed rate was adjusted by a feedforward/feedback controller such that the specific growth rate was kept on the desired set-point value. On the basis of experimental investigations it was demonstrated that the specific growth rate can be controlled at a given set point value below the critical value to prevent the production of growth-inhibitory ethanol due to the Crabtree effect. With this control strategy high biomass concentrations of more than 110 g l(-1) can be obtained.
authors.affiliation: University of Applied Sciences Esslingen, Department of Natural Sciences, Kanalstrasse 33, 73728 Esslingen, Germany. richard.biener@hs-esslingen.de
authors.forename: Richard
authors.initials: R
authors.lastname: Biener
authors.forename: Anne
authors.initials: A
authors.lastname: Steinkämper
authors.forename: Thomas
authors.initials: T
authors.lastname: Horn
mesh_terms: D001676:Biofeedback, Psychology / Q000502:physiology*; D019149:Bioreactors / Q000382:microbiology*; D002151:Calorimetry / Q000295:instrumentation*; D049109:Cell Proliferation; D003198:Computer Simulation; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005947:Glucose / Q000378:metabolism*; D006361:Heating / Q000295:instrumentation*; D008954:Models, Biological; D012441:Saccharomyces cerevisiae / Q000502:physiology*
chemical_list: D005947:Glucose