title: Regulation of spermatozoa motility in response to cations in Russian sturgeon Acipenser gueldenstaedtii.
abstract: The aim of this study was to investigate the response of Russian sturgeon (Acipenser gueldenstaedtii) sperm to external cations (Na(+), K(+), Ca(2+), and Mg(2+)) and their susceptibility on the induction of motility and swimming behavior. An in vitro spermatozoa motility assay was used by a computer-aided Motion-Analysis system. Sperm motility was inhibited by 60 mM NaCl (~140 mOsm/kg) and 0.7 mM KCl solutions (~ 21.4 mOsm/kg). The Ca(2+) and Mg(2+) ions were not able to inhibit spermatozoa motility. By contrast, Na(+) within a limited concentration range (between 45 and 55 mm) was able to reverse the inhibitory effect of K(+) at the critical concentration (0.7 mM). Ca(2+) and Mg(2+) were also able to reverse the K(+)-mediated spermatozoa motility restriction at concentrations starting at 0.01 and 0.1 mM, respectively. These results provide evidence for the role of K(+) in suppressing spermatozoa motility, and suggest that Ca(2+), Mg(2+), and possibly Na(+) trigger motility in Russian sturgeon sperm.
authors.affiliation: University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, Zatisi 728/II, Vodnany, Czech Republic. pingli06@yahoo.com
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Zhi-Hua
authors.initials: ZH
authors.lastname: Li
authors.forename: M
authors.initials: M
authors.lastname: Hulak
authors.forename: M
authors.initials: M
authors.lastname: Rodina
authors.forename: O
authors.initials: O
authors.lastname: Linhart
mesh_terms: D000818:Animals; D017756:Aquaculture; D002118:Calcium / Q000494:pharmacology; D002412:Cations / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005399:Fishes / Q000502:physiology; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D009994:Osmolar Concentration; D011188:Potassium / Q000494:pharmacology; D055101:Semen Analysis; D012964:Sodium / Q000494:pharmacology; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects / Q000502:physiology; D013550:Swimming
chemical_list: D002412:Cations; D012964:Sodium; D008274:Magnesium; D011188:Potassium; D002118:Calcium