title: Application of collagen hydrogel/sponge scaffold facilitates periodontal wound healing in class II furcation defects in beagle dogs.
abstract: OBJECTIVE
  A three-dimensional scaffold may play an important role in periodontal tissue engineering. We prepared bio-safe collagen hydrogel, which exhibits properties similar to those of native extracellular matrix. The aim of this study was to examine the effect of implantation of collagen hydrogel/sponge scaffold on periodontal wound healing in class II furcation defects in dogs.
  
  
  METHODS
  The collagen hydrogel/sponge scaffold was prepared by injecting collagen hydrogel, cross-linked to the ascorbate-copper ion system, into a collagen sponge. Class II furcation defects (of 5 mm depth and 3 mm width) were surgically created in beagle dogs. The exposed root surface was planed and demineralized with EDTA. In the experimental group, the defect was filled with collagen hydrogel/sponge scaffold. In the control group, no implantation was performed. Histometric parameters were evaluated 2 and 4 wk after surgery.
  
  
  RESULTS
  At 2 wk, the collagen hydrogel/sponge scaffold displayed high biocompatibility and biodegradability with numerous cells infiltrating the scaffold. In the experimental group, reconstruction of alveolar bone and cementum was frequently observed 4 wk after surgery. Periodontal ligament tissue was also re-established between alveolar bone and cementum. Volumes of new bone, new cementum and new periodontal ligament were significantly greater in the experimental group than in the control group. In addition, epithelial down-growth was suppressed by application of collagen hydrogel.
  
  
  CONCLUSIONS
  The collagen hydrogel/sponge scaffold possessed high tissue compatibility and degradability. Implantation of the scaffold facilitated periodontal wound healing in class II furcation defects in beagle dogs.
authors.affiliation: Department of Periodontology and Endodontology, Division of Oral Health Science, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kosen
authors.forename: H
authors.initials: H
authors.lastname: Miyaji
authors.forename: A
authors.initials: A
authors.lastname: Kato
authors.forename: T
authors.initials: T
authors.lastname: Sugaya
authors.forename: M
authors.initials: M
authors.lastname: Kawanami
mesh_terms: D020341:Absorbable Implants; D000539:Alveolar Process / Q000473:pathology; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D025021:Cementogenesis / Q000502:physiology; D002614:Chelating Agents / Q000627:therapeutic use; D003094:Collagen / Q000737:chemistry*; D003238:Connective Tissue / Q000254:growth & development / Q000473:pathology; D003739:Dental Cementum / Q000473:pathology; D004285:Dogs; D004492:Edetic Acid / Q000627:therapeutic use; D004846:Epithelial Attachment / Q000254:growth & development / Q000473:pathology; D005260:Female; D024022:Fibrillar Collagens / Q000737:chemistry; D005347:Fibroblasts / Q000473:pathology; D017823:Furcation Defects / Q000473:pathology / Q000601:surgery*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D010006:Osteoblasts / Q000473:pathology; D010012:Osteogenesis / Q000502:physiology; D010513:Periodontal Ligament / Q000254:growth & development / Q000473:pathology; D011897:Random Allocation; D016745:Root Planing / Q000379:methods; D013997:Time Factors; D054457:Tissue Scaffolds / Q000737:chemistry*; D014092:Tooth Root / Q000473:pathology / Q000601:surgery; D014945:Wound Healing / Q000502:physiology
chemical_list: D001672:Biocompatible Materials; D002614:Chelating Agents; D024022:Fibrillar Collagens; C059821:atelocollagen; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D003094:Collagen; D004492:Edetic Acid