title: [Association of hOGG1 genotype with life style and oxidative DNA damage in five ethnic populations in China].
abstract: OBJECTIVE
  To assess the natural variation of hOGG1 gene and the gene-environmental interactions.
  
  
  METHODS
  The hOGG1 codon 326 polymorphism and urinary 8-OHdG levels were investigated in a large sample of healthy individuals (n = 953) from five ethnic populations in China by using PCR-RFLP and HPLC-ECD. Life style parameters were obtained through a questionnaire survey.
  
  
  RESULTS
  The allelic frequencies of hOGG1 gene in whole subjects were 0.16 (Ser/Ser), 0.49 (Ser/Cys) and 0.35 (Cys/Cys). The frequency of Ser326Cys was significantly different among the five ethnic populations (P = 0.002). No association was found between the hOGG1 gene polymorphism and other life style parameters except for the association between Ser326Cys and smoking (P = 0.027). A significant increase of urinary 8-OHdG level was observed in Cys326Cys allelic healthy subjects (P = 0.033).
  
  
  CONCLUSIONS
  There are natural variations of hOGG1 gene in different ethnic populations in China. Smoking is related to the frequencies of Cys/Cys polymorphism, and the repairing of oxidative DNA damage is lower in individuals with the hOGG1 Cys326Cys genotype.
authors.affiliation: Shenzhen Center for Diseases Control and Prevention, Shenzhen 518055, China. wishesbb@hotmail.com
authors.forename: Shuang
authors.initials: S
authors.lastname: Wu
authors.forename: Yousheng
authors.initials: Y
authors.lastname: Jiang
authors.forename: Jianhui
authors.initials: J
authors.lastname: Yuan
authors.forename: Zhixiong
authors.initials: Z
authors.lastname: Zhuang
authors.forename: Yuebin
authors.initials: Y
authors.lastname: Ke
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D002681:China / Q000208:ethnology; D004249:DNA Damage; D045647:DNA Glycosylases / Q000235:genetics*; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000652:urine; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008019:Life Style; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D011795:Surveys and Questionnaires
chemical_list: D000080242:8-Hydroxy-2'-Deoxyguanosine; D045647:DNA Glycosylases; C479058:oxoguanine glycosylase 1, human; D003849:Deoxyguanosine