title: Jeju ground water containing vanadium induced immune activation on splenocytes of low dose γ-rays-irradiated mice.
abstract: Vanadium, an essential micronutrient, has been implicated in controlling diabetes and carcinogenesis and in impeding reactive oxygen species (ROS) generation. γ-ray irradiation triggers DNA damage by inducing ROS production and causes diminution in radiosensitive immunocytes. In this study, we elucidate the immune activation capacities of Jeju water containing vanadium on immunosuppression caused by γ-ray irradiation, and identify its mechanism using various low doses of NaVO(3). We examined the intracellular ROS generation, DNA damage, cell proliferation, population of splenocytes, and cytokine/antibody profiles in irradiated mice drinking Jeju water for 180 days and in non-irradiated and in irradiated splenocytes both of which were treated with NaVO(3). Both Jeju water and 0.245 μM NaVO(3) attenuated the intracellular ROS generation and DNA damage in splenocytes against γ-ray irradiation. Splenocytes were significantly proliferated by the long-term intake of Jeju water and by 0.245 μM NaVO(3) treatment, and the expansion of B cells accounted for the increased number of splenocytes. Also, 0.245 μM NaVO(3) treatment showed the potency to amplify the production of IFN-γ and total IgG in irradiated splenocytes, which correlated with the expansion of B cells. Collectively, Jeju water containing vanadium possesses the immune activation property against damages caused by γ-irradiation.
authors.affiliation: College of Veterinary Medicine and Applied Radiological Science Institute, Jeju National University, Jeju, South Korea.
authors.forename: Danbee
authors.initials: D
authors.lastname: Ha
authors.forename: Haejin
authors.initials: H
authors.lastname: Joo
authors.forename: Ginnae
authors.initials: G
authors.lastname: Ahn
authors.forename: Min Ju
authors.initials: MJ
authors.lastname: Kim
authors.forename: So Jin
authors.initials: SJ
authors.lastname: Bing
authors.forename: Subin
authors.initials: S
authors.lastname: An
authors.forename: Hyunki
authors.initials: H
authors.lastname: Kim
authors.forename: Kyung-goo
authors.initials: KG
authors.lastname: Kang
authors.forename: Yoon-Kyu
authors.initials: YK
authors.lastname: Lim
authors.forename: Youngheun
authors.initials: Y
authors.lastname: Jee
mesh_terms: D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D001402:B-Lymphocytes / Q000187:drug effects / Q000528:radiation effects; D003037:Cobalt Radioisotopes; D004396:Coloring Agents; D020552:Comet Assay; D016207:Cytokines / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D005452:Fluoresceins; D016166:Free Radical Scavengers; D005720:Gamma Rays; D060587:Groundwater / Q000032:analysis*; D007371:Interferon-gamma / Q000096:biosynthesis; D051379:Mice; D017382:Reactive Oxygen Species / Q000378:metabolism; D056910:Republic of Korea; D013154:Spleen / Q000166:cytology / Q000276:immunology* / Q000528:radiation effects*; D013778:Tetrazolium Salts; D013844:Thiazoles; D013936:Thymidine / Q000378:metabolism; D014638:Vanadates / Q000494:pharmacology; D014639:Vanadium / Q000009:adverse effects* / Q000032:analysis*
chemical_list: D003037:Cobalt Radioisotopes; D004396:Coloring Agents; D016207:Cytokines; D005452:Fluoresceins; D016166:Free Radical Scavengers; D017382:Reactive Oxygen Species; D013778:Tetrazolium Salts; D013844:Thiazoles; D014639:Vanadium; C029569:diacetyldichlorofluorescein; D014638:Vanadates; D007371:Interferon-gamma; C022616:thiazolyl blue; D013936:Thymidine