title: A novel halogen bond and a better-known hydrogen bond cooperation of neonicotinoid and insect nicotinic acetylcholine receptor recognition.
abstract: Neonicotinoid insecticides target the insect nicotinic acetylcholine receptor (nAChR) and are highly effective against the piercing-sucking pests. To explore the molecular interaction mechanism between the neonicotinoids and the insect nAChR, some key neonicotinoid compounds were docked into Aplysia californica acetylcholine binding protein (Ac-AChBP), which serves as a suitable structural surrogate of the insect nAChR. The binding mode study showed that the hydrogen bond force between the electronegative pharmacophore of the neonicotinoids and Cys190NH of the target binding pocket is crucial to the high efficiency of the neonicotinoids. Increasing the coplanarity between the guanidine or amidine and the electronegative pharmacophore of the neonicotinoids could increase the Π-Π stacking effect with Tyr188 of the Ac-AChBP and thus enhance the insecticidal potency. The introduction of an azide group to the chloropyridine ring of the neonicotinoids would reduce its binding ability due to the disappearance of a novel halogen bonding interaction. A series of novel neonicotinoid molecules were designed based on the halogen bonding interaction and two compounds with 6-bromopyridine-3-yl and 6-(trifluoromethyl)-3-pyridinyl were found to be with potential insecticidal activities.
authors.affiliation: Department of Applied Chemistry, College of Science, Key Laboratory of Pesticide Chemistry and Application Technology, China Agricultural University, Beijing, 100193, People's Republic of China. hxduan@cau.edu.cn
authors.forename: Hongxia
authors.initials: H
authors.lastname: Duan
authors.forename: Weiwei
authors.initials: W
authors.lastname: Zhang
authors.forename: Jin
authors.initials: J
authors.lastname: Zhao
authors.forename: Desheng
authors.initials: D
authors.lastname: Liang
authors.forename: Xinling
authors.initials: X
authors.lastname: Yang
authors.forename: Shuhui
authors.initials: S
authors.lastname: Jin
mesh_terms: D000578:Amidines / Q000737:chemistry; D001665:Binding Sites; D006146:Guanidines / Q000737:chemistry; D006846:Hydrocarbons, Halogenated / Q000737:chemistry*; D006860:Hydrogen Bonding; D019476:Insect Proteins / Q000737:chemistry*; D007306:Insecticides / Q000737:chemistry*; D018733:Nicotinic Antagonists / Q000737:chemistry*; D011485:Protein Binding; D011725:Pyridines / Q000737:chemistry; D011978:Receptors, Nicotinic / Q000737:chemistry*
chemical_list: D000578:Amidines; D006146:Guanidines; D006846:Hydrocarbons, Halogenated; D019476:Insect Proteins; D007306:Insecticides; D018733:Nicotinic Antagonists; D011725:Pyridines; D011978:Receptors, Nicotinic