title: The effect of allogenetic bone marrow-derived mesenchymal stem cell transplantation on lung aquaporin-1 and -5 in a rat model of severe acute pancreatitis.
abstract: OBJECTIVE
  We aimed to determine the effect of transplantation on post- ALI (acute lung injury) edema in severe acute pancreatitis (SAP) and the expression levels of aquaporins -1 and -5 (AQP-1 and -5).
  
  
  METHODS
  Sprague-Dawley (SD) rats were randomized into control-SAP and BMSCs-SAP groups. SAP model was prepared through retrograde injection of 5% taurocholic acid. BMSCs were isolated from the bone marrow of SD rats. We examined SAP rats for levels of IL-1β and TNF-a, and for AQP-1 and -5 expression in lung tissues at 6 and 12 hours.
  
  
  RESULTS
  The levels of IL-1β and TNF-a in BMSC-SAP rats were lower than in control-SAP rats (both, p<0.001). Real-time RT-PCR analysis showed that AQP-1 mRNA expression in BMSC-SAP rats was higher than that in control-SAP rats (p=0.005 and p<0.001), and AQP-5 mRNA expression in BMSC-SAP rats was also higher than that in control-SAP rats (p=0.031 and p=0.006). Western blotting analysis showed that AQP-1 and AQP-5 protein levels at 12h were significantly higher in BMSC-SAP rats than in control-SAP rats (p<0.001).
  
  
  CONCLUSIONS
  Allogenic BMSC transplantation can protect against ALI in a rat SAP model and can also regulate the expression levels of AQP-1 and -5 by inhibiting IL-1β and TNF-a.
authors.affiliation: Department of Emergency Surgery, Fujian Medical University, Fujian, China.
authors.forename: Qiaoyi
authors.initials: Q
authors.lastname: Wu
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Yuling
authors.initials: Y
authors.lastname: Hou
authors.forename: Shuiping
authors.initials: S
authors.lastname: Chen
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Fengchun
authors.initials: F
authors.lastname: Lu
authors.forename: Heguang
authors.initials: H
authors.lastname: Huang
authors.forename: Yanchang
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000208:Acute Disease; D000681:Amylases / Q000097:blood; D000818:Animals; D051398:Aquaporin 1 / Q000032:analysis* / Q000235:genetics; D051402:Aquaporin 5 / Q000032:analysis* / Q000235:genetics; D002478:Cells, Cultured; D004195:Disease Models, Animal; D008168:Lung / Q000737:chemistry* / Q000378:metabolism / Q000473:pathology; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D009929:Organ Size; D010195:Pancreatitis / Q000378:metabolism / Q000628:therapy*; D010539:Permeability; D051381:Rats; D017207:Rats, Sprague-Dawley; D012709:Serum Albumin / Q000032:analysis; D014184:Transplantation, Homologous
chemical_list: C496022:Aqp1 protein, rat; C496040:Aqp5 protein, rat; D051402:Aquaporin 5; D012709:Serum Albumin; D051398:Aquaporin 1; D000681:Amylases