title: Production of GM-CSF mediated by cysteine protease of Der f in canine keratinocytes.
abstract: House dust mite (HDM) allergens are the most common allergens for induction of IgE-mediated hypersensitivity. Recently, epicutaneous sensitization with HDM allergens has been emphasized in the development of atopic dermatitis (AD) by producing various soluble factors in keratinocytes. Among the soluble factors, GM-CSF is a key molecule that activates Langerhans cells, antigen-presenting cells in the epidermis. In the present study, we investigated the effects of Dermatophagoides farinae (Der f) on GM-CSF production in a canine keratinocyte cell line, CPEK. CPEKs were found to produce GM-CSF upon stimulation by Der f. The GM-CSF production was suppressed by addition of a cysteine protease inhibitor. The present results suggest that cysteine protease-derived Der f may be an initiator of allergic inflammation by inducing the production of GM-CSF in keratinocytes.
authors.affiliation: The United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Kimura
authors.forename: Machiko
authors.initials: M
authors.lastname: Sekido
authors.forename: Naoki
authors.initials: N
authors.lastname: Chimura
authors.forename: Sanae
authors.initials: S
authors.lastname: Shibata
authors.forename: Naho
authors.initials: N
authors.lastname: Kondo
authors.forename: Harumi
authors.initials: H
authors.lastname: Kamishina
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kamishina
authors.forename: Sadatoshi
authors.initials: S
authors.lastname: Maeda
mesh_terms: D000485:Allergens / Q000494:pharmacology*; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000494:pharmacology*; D018929:Cell Culture Techniques; D002460:Cell Line; D057056:Cysteine Proteases / Q000235:genetics / Q000378:metabolism*; D004285:Dogs / Q000378:metabolism*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism*; D015603:Keratinocytes / Q000187:drug effects* / Q000201:enzymology*
chemical_list: D000485:Allergens; D039741:Antigens, Dermatophagoides; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D057056:Cysteine Proteases