title: Solid-state spectroscopic characterization of α-chitins deacetylated in homogeneous solutions.
abstract: Synthesis and spectroscopic characterization of α-chitins exhibiting degrees of acetylation (DA) between 0.5 and 1 were reported. Crab shell α-chitin consisting of microfibrils with a width of less than 1 μm was regenerated from or alkaline deacetylated in 30% aqueous NaOH solution. After the deacetylation in homogeneous solution, the DA of α-chitins decreased steadily with prolonged deacetylation times from 1 to 0.54. The native microfibrils of starting α-chitin were destroyed, while aggregates or sheets were formed depending on the DA of chitins. According to WAXD measurements, deacetylated chitins with DA lower than 0.76 have different crystalline structures than starting α-chitin. Furthermore, FT Raman spectroscopy demonstrated a novel rapid method to determine the DA of chitin with correlation coefficients greater than 0.99. On the basis of the FT Raman signals ascribed to the amide groups, the amount of hydrogen bonds linked to C═O groups decreased strongly with lower DA and the relationship between them was presented.
authors.affiliation: Institute of Plant and Wood Chemistry, Dresden University of Technology, Tharandt, Germany. kzhang@forst.tu-dresden.de
authors.forename: Kai
authors.initials: K
authors.lastname: Zhang
authors.forename: Andreas
authors.initials: A
authors.lastname: Geissler
authors.forename: Steffen
authors.initials: S
authors.lastname: Fischer
authors.forename: Erica
authors.initials: E
authors.lastname: Brendler
authors.forename: Ernst
authors.initials: E
authors.lastname: Bäucker
mesh_terms: D000107:Acetylation; D000577:Amides / Q000737:chemistry; D000818:Animals; D002686:Chitin / Q000737:chemistry* / Q000648:ultrastructure; D033364:Decapoda; D006860:Hydrogen Bonding; D008855:Microscopy, Electron, Scanning; D012996:Solutions / Q000737:chemistry; D013059:Spectrum Analysis, Raman
chemical_list: D000577:Amides; D012996:Solutions; D002686:Chitin