title: Purification of Tetrahymena cytoskeletal proteins.
abstract: Like all eukaryotic cells, Tetrahymena thermophila contains a rich array of cytoskeletal proteins, some familiar and some novel. A detailed analysis of the structure, function, and interactions of these proteins requires procedures for purifying the individual protein components. Procedures for the purification of actin and tubulin from Tetrahymena are reviewed, followed by a description of a procedure that yields proteins from the epiplasmic layer and associated structures, including the tetrins. Finally, the challenges and opportunities for future advances are assessed.
authors.affiliation: Department of Biology, Drake University, 2507 University Avenue, Des Moines, IA, USA.
authors.forename: Jerry E
authors.initials: JE
authors.lastname: Honts
mesh_terms: D000199:Actins / Q000737:chemistry / Q000302:isolation & purification*; D002462:Cell Membrane / Q000737:chemistry; D002923:Cilia / Q000737:chemistry; D003598:Cytoskeletal Proteins / Q000737:chemistry / Q000302:isolation & purification*; D003599:Cytoskeleton / Q000737:chemistry; D004398:Dyneins / Q000737:chemistry / Q000302:isolation & purification; D008565:Membrane Proteins / Q000737:chemistry / Q000302:isolation & purification; D009218:Myosins / Q000737:chemistry / Q000302:isolation & purification; D040901:Proteomics / Q000379:methods; D015800:Protozoan Proteins / Q000737:chemistry / Q000302:isolation & purification*; D012995:Solubility; D016808:Tetrahymena thermophila / Q000737:chemistry*; D014404:Tubulin / Q000737:chemistry / Q000302:isolation & purification*; D014461:Ultracentrifugation
chemical_list: D000199:Actins; D003598:Cytoskeletal Proteins; D008565:Membrane Proteins; D015800:Protozoan Proteins; D014404:Tubulin; D009218:Myosins; D004398:Dyneins