title: Effect of RECK gene polymorphisms on hepatocellular carcinoma susceptibility and clinicopathologic features.
abstract: BACKGROUND
  The reversion-inducing-cysteine-rich protein with Kazal motifs (RECK) down-regulation has been confirmed in numerous human cancers and is clinically associated with metastasis. This study investigates the potential associations of RECK single-nucleotide polymorphisms (SNPs) with hepatocellular carcinoma (HCC) susceptibility and its clinicopathologic characteristics.
  
  
  RESULTS
  A total of 135 HCC cancer patients and 501 cancer-free controls were analyzed for four RECK SNPs (rs10814325, rs16932912, rs11788747, and rs10972727) using real-time PCR and PCR-RFLP genotyping analysis. After adjusting for other co-variants, the individuals carrying RECK promoter rs10814325 inheriting at least one C allele had a 1.85-fold [95% confidence interval (CI), 1.03-3.36] risk of developing HCC compared to TT wild type carriers. The HCC patients, who carried rs11788747 with at least one G allele, had a higher distant metastasis risk than wild type probands.
  
  
  CONCLUSIONS
  RECK gene polymorphisms might be a risk factor increasing HCC susceptibility and distant metastasis in Taiwan.
authors.affiliation: Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan.
authors.forename: Tsung-Te
authors.initials: TT
authors.lastname: Chung
authors.forename: Chao-Bin
authors.initials: CB
authors.lastname: Yeh
authors.forename: Yi-Ching
authors.initials: YC
authors.lastname: Li
authors.forename: Shih-Chi
authors.initials: SC
authors.lastname: Su
authors.forename: Ming-Hsien
authors.initials: MH
authors.lastname: Chien
authors.forename: Shun-Fa
authors.initials: SF
authors.lastname: Yang
authors.forename: Yi-Hsien
authors.initials: YH
authors.lastname: Hsieh
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000453:epidemiology* / Q000235:genetics*; D016022:Case-Control Studies; D017931:DNA Primers / Q000235:genetics; D058851:GPI-Linked Proteins / Q000235:genetics*; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D006801:Humans; D008113:Liver Neoplasms / Q000453:epidemiology* / Q000235:genetics*; D016015:Logistic Models; D009362:Neoplasm Metastasis / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012307:Risk Factors; D018709:Statistics, Nonparametric; D013624:Taiwan / Q000453:epidemiology
chemical_list: D017931:DNA Primers; D058851:GPI-Linked Proteins; C115460:RECK protein, human