title: Reversal of multidrug resistance of KBV200 cells by triterpenoids isolated from Poria cocos.
abstract: Multidrug resistance (MDR) of tumor cells is one of the major problems encountered during cancer chemotherapy. In this paper, we isolated eight triterpenoids from Poria cocos and evaluated their effects on reversing MDR of KBV200 cells. Eight triterpenoids increase significantly vincristine-induced cytotoxicity in drug-resistant KBV200 cells at the concentrations of 12.5 µg/mL and 25 µg/mL. Dehydrotumulosic acid showed the best reversal effect: it increased KBV200 apoptosis induced by vincristine and inhibited P-gp function through enhancing the accumulation and retention of fluorescent P-gp substrate rhodamine 123 in KBV200 cells but had no effect on P-gp expression.
authors.affiliation: Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing, People's Republic of China.
authors.forename: He
authors.initials: H
authors.lastname: Shan
authors.forename: Zhang
authors.initials: Z
authors.lastname: Qinglin
authors.forename: Xiao
authors.initials: X
authors.lastname: Fengjun
authors.forename: Lu
authors.initials: L
authors.lastname: Yuxin
authors.forename: Cheng
authors.initials: C
authors.lastname: Xiaochen
authors.forename: Han
authors.initials: H
authors.lastname: Yuan
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects*; D001681:Biological Assay; D002457:Cell Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D018432:Drug Resistance, Multiple / Q000187:drug effects*; D006801:Humans; D008516:Medicine, Chinese Traditional; D015394:Molecular Structure; D055441:Poria / Q000737:chemistry*; D020112:Rhodamine 123 / Q000378:metabolism; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D014750:Vincristine / Q000494:pharmacology
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D002457:Cell Extracts; D014315:Triterpenes; D020112:Rhodamine 123; D014750:Vincristine; C102488:dehydrotumulosic acid