title: Effects of hypothermia on NSE and S-100 protein levels in CSF in neonates following hypoxic/ischaemic brain damage.
abstract: OBJECTIVE
  The aim of the study was to evaluate the effects of hypothermia on neuron-specific enolase (NSE) and S-100 protein levels in cerebrospinal fluid (CSF) in neonates with hypoxic/ischaemic encephalopathy (HIE).
  
  
  METHODS
  Fifty-one enrolled neonates with HIE were divided into two groups: hypothermia (n = 23) and control (n = 28). NSE and S-100 protein were measured with immunoradiometric assays. Amino acid neurotransmitters were also measured by reversed-phase high-performance liquid chromatography. Neurodevelopmental assessments were performed at 3 and 12 months of age.
  
  
  RESULTS
  Neuron-specific enolase and S-100 levels were lower, and neurodevelopment outcome was better in the hypothermia group compared with the control group. Among the infants who received hypothermia, CSF NSE and S-100 were significantly higher in those who developed severe neurological impairment (mental development index or physical development index <70). There were no significant differences between the two groups in amino acid neurotransmitters.
  
  
  CONCLUSIONS
  These results indicated that hypothermia was associated with decreased CSF NSE and S-100 level and correlated with neurodevelopmental outcome in infants with HIE.
authors.affiliation: Department of Clinical Immunology, Children's Hospital of Fudan University, Shanghai, China. jinqiao1977@yahoo.com.cn
authors.forename: Jinqiao
authors.initials: J
authors.lastname: Sun
authors.forename: Jin
authors.initials: J
authors.lastname: Li
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Cheng
authors.forename: Bin
authors.initials: B
authors.lastname: Sha
authors.forename: Wenhao
authors.initials: W
authors.lastname: Zhou
mesh_terms: D000596:Amino Acids / Q000134:cerebrospinal fluid; D015415:Biomarkers / Q000134:cerebrospinal fluid; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007036:Hypothermia, Induced; D020925:Hypoxia-Ischemia, Brain / Q000134:cerebrospinal fluid / Q000628:therapy*; D015592:Immunoradiometric Assay; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D009483:Neuropsychological Tests; D010751:Phosphopyruvate Hydratase / Q000134:cerebrospinal fluid*; D009418:S100 Proteins / Q000134:cerebrospinal fluid*; D012720:Severity of Illness Index; D016896:Treatment Outcome
chemical_list: D000596:Amino Acids; D015415:Biomarkers; D009418:S100 Proteins; D010751:Phosphopyruvate Hydratase