title: High hepatitis B virus infection in B-cell lymphoma tissue and its potential clinical relevance.
abstract: Our previous studies found that patients with B-cell non-Hodgkin lymphoma (NHL) had a higher incidence of hepatitis B virus (HBV) infection in serum than patients with T-cell NHL or other cancers. We sought to identify a possible role of HBV infection in B-cell NHL tumorigenesis and to understand its underlying clinical relevance. Fresh and paraffin-embedded primary tumor tissue from patients with NHL as well as from those with other lymphatic system diseases were investigated by PCR and immunohistochemistry. Many more patients with B-cell lymphoma whose serum was positive for hepatitis B surface antigen (HBsAg) were also positive for HBV-DNA than were those with T-cell NHL or other lymphatic system diseases whose serum was positive for HBsAg, in both fresh (55 vs. 15.4%) and paraffin-embedded (38.3 vs. 11.8%) tissue. Positive expression of the HBV-associated proteins HBsAg and hepatitis B core antigen was found in B-cell NHL lymphocytes and endothelial cells. Only 8.3% of patients with B-cell NHL who were negative for HBsAg but positive for other HBV markers were positive for HBV-DNA in tumor tissue. These results suggest that chronic HBV infection in lymph nodes could be associated with B-cell lymphoma.
authors.affiliation: Department of Medical Oncology, Sun Yat-Sen University Cancer Center, Guangdong, Guangzhou, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Shuqiang
authors.initials: S
authors.lastname: Yuan
authors.forename: Kai-yuan
authors.initials: KY
authors.lastname: Teng
authors.forename: Celia
authors.initials: C
authors.lastname: Garcia-Prieto
authors.forename: Hui-yan
authors.initials: HY
authors.lastname: Luo
authors.forename: Mu-sheng
authors.initials: MS
authors.lastname: Zeng
authors.forename: Hui-lan
authors.initials: HL
authors.lastname: Rao
authors.forename: Yi
authors.initials: Y
authors.lastname: Xia
authors.forename: Wen-qi
authors.initials: WQ
authors.lastname: Jiang
authors.forename: Hui-qiang
authors.initials: HQ
authors.lastname: Huang
authors.forename: Zhong-jun
authors.initials: ZJ
authors.lastname: Xia
authors.forename: Xiao-fei
authors.initials: XF
authors.lastname: Sun
authors.forename: Rui-hua
authors.initials: RH
authors.lastname: Xu
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D004279:DNA, Viral / Q000097:blood*; D005260:Female; D005500:Follow-Up Studies; D006512:Hepatitis B Core Antigens / Q000097:blood*; D006514:Hepatitis B Surface Antigens / Q000097:blood*; D006515:Hepatitis B virus / Q000235:genetics / Q000276:immunology; D019694:Hepatitis B, Chronic / Q000097:blood / Q000821:virology*; D006801:Humans; D016393:Lymphoma, B-Cell / Q000097:blood / Q000821:virology*; D008297:Male; D008875:Middle Aged; D055815:Young Adult
chemical_list: D015415:Biomarkers; D004279:DNA, Viral; D006512:Hepatitis B Core Antigens; D006514:Hepatitis B Surface Antigens