title: Aqueous extracts of Lippia turbinata and Aloysia citriodora (Verbenaceae): assessment of antioxidant capacity and DNA damage.
abstract: The aim of the present work was to make a contribution to the knowledge of aqueous extracts of Lippia turbinata and Aloysia citriodora (Verbenaceae; infusion and decoction) in relation with the establishment of its antioxidant activity and lack of DNA damage, for its potential use in therapeutics. The cytogenotoxic profile was evaluated through genotoxic biomarkers such as mitotic index, cellular proliferation kinetics, sister chromatid exchanges, single-cell gel electrophoresis assay, and micronucleus test in human peripheral blood lymphocyte cultures. No statistical differences were found (P > .05) between control and exposed cultures, even between both aqueous extracts. The total antioxidant capacity was shown to be higher in the decoction than in the infusion and both aqueous extracts protected against protein carbonylation and lipid peroxidation, the decoction being more efficient than the infusion (P < .005). These results suggest the safe use of these medicinal plants as chemoecologic agents in therapeutics.
authors.affiliation: CIGETOX-Citogenética y Genética Toxicológica-INFIBIOC, Instituto de Fisiopatología y Bioquímica Clínica, Facultad de Farmacia y Bioquímica, UBA, Buenos Aires, Argentina.
authors.forename: Erika
authors.initials: E
authors.lastname: Portmann
authors.forename: Marcela M López
authors.initials: MM
authors.lastname: Nigro
authors.forename: Claudia G
authors.initials: CG
authors.lastname: Reides
authors.forename: Susana
authors.initials: S
authors.lastname: Llesuy
authors.forename: Rafael A
authors.initials: RA
authors.lastname: Ricco
authors.forename: Marcelo L
authors.initials: ML
authors.lastname: Wagner
authors.forename: Alberto A
authors.initials: AA
authors.lastname: Gurni
authors.forename: Marta A
authors.initials: MA
authors.lastname: Carballo
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001921:Brain / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D020552:Comet Assay; D004249:DNA Damage; D005260:Female; D005419:Flavonoids / Q000032:analysis; D006801:Humans; D008163:Luminescent Measurements; D008214:Lymphocytes; D015162:Micronucleus Tests; D008940:Mitotic Index; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D059808:Polyphenols / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D012854:Sister Chromatid Exchange / Q000187:drug effects; D013634:Tannins / Q000032:analysis; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D029648:Verbenaceae / Q000737:chemistry
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D010936:Plant Extracts; D059808:Polyphenols; D013634:Tannins; D017392:Thiobarbituric Acid Reactive Substances