title: Vaginal carriage rate of group B Streptococcus in pregnant women and its transmission to neonates.
abstract: BACKGROUND
  Maternal vaginal colonisation with Group B Streptococcus (GBS, Streptococcus agalactiae) at the time of delivery can cause vertical transmission to the neonate. GBS is the leading cause of sepsis, meningitis and pneumonia in the infants. Asymptomatic colonisation of the vagina with GBS varies with the geographical location.
  
  
  METHODS
  This was a cross-sectional study conducted in 2009 at Benazir Bhutto Hospital, Rawalpindi, Pakistan. Lower vaginal swabs were obtained from 200 pregnant women at the time of admission in the Gynaecology and Obstetrics Department for term, normal vaginal delivery and swabs from the skin of abdomen and ear canals of their respective neonates immediately after delivery were collected. Swabs were inoculated on blood agar and incubated aerobically and on Group B Streptococcus agar (GBS agar) and incubated anaerobically in an anaerobic jar. Identification of GBS was made on the basis of colonial morphology (beta-haemolytic colonies on blood agar and orange pigmented colonies on GBS agar), Gram stain, catalase test and conformation was done by means of latex agglutination tests.
  
  
  RESULTS
  A GBS carriage rate of 8.5% among pregnant women before delivery and an acquisition rate of 53% on the abdominal skin and 18% in the ear canals by the neonates of colonised mothers were found.
  
  
  CONCLUSIONS
  GBS colonisation in pregnant women and its transmission to the neonates is present in our population so GBS infections in the prenatal and neonatal period might not be uncommon in Pakistan, so routine screening should be carried out.
authors.affiliation: Department of Pathology, Rawalpindi Medical College, Rawalpindi, Pakistan. drbushrazaheer@yahoo.com
authors.forename: Bushra Yasmin
authors.initials: BY
authors.lastname: Chaudhry
authors.forename: Naeem
authors.initials: N
authors.lastname: Akhtar
authors.forename: Abbas Hayat
authors.initials: AH
authors.lastname: Balouch
mesh_terms: D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D007232:Infant, Newborn, Diseases / Q000453:epidemiology; D018445:Infectious Disease Transmission, Vertical; D010154:Pakistan / Q000453:epidemiology; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious; D013292:Streptococcus agalactiae / Q000302:isolation & purification*; D014621:Vagina / Q000382:microbiology*