title: Breast cancer exosome-like microvesicles and salivary gland cells interplay alters salivary gland cell-derived exosome-like microvesicles in vitro.
abstract: Saliva is a useful biofluid for the early detection of disease, but how distal tumors communicate with the oral cavity and create disease-specific salivary biomarkers remains unclear. Using an in vitro breast cancer model, we demonstrated that breast cancer-derived exosome-like microvesicles are capable of interacting with salivary gland cells, altering the composition of their secreted exosome-like microvesicles. We found that the salivary gland cells secreted exosome-like microvesicles encapsulating both protein and mRNA. We also showed that the interaction with breast cancer-derived exosome-like microvesicles communicated and activated the transcriptional machinery of the salivary gland cells. Thus, the interaction altered the composition of the salivary gland cell-derived exosome-like microvesicles on both the transcriptomically and proteomically.
authors.affiliation: University of California Los Angeles School of Dentistry and Dental Research Institute, Los Angeles, California, United States of America.
authors.forename: Chang S
authors.initials: CS
authors.lastname: Lau
authors.forename: David T W
authors.initials: DT
authors.lastname: Wong
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology*; D055252:Cell-Derived Microparticles / Q000378:metabolism*; D002478:Cells, Cultured; D055354:Exosomes / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics; D012463:Saliva / Q000737:chemistry; D012469:Salivary Glands / Q000378:metabolism* / Q000473:pathology*; D014158:Transcription, Genetic
chemical_list: D014408:Biomarkers, Tumor; D012333:RNA, Messenger