title: Acylsucrose-producing tomato plants forces Bemisia tabaci to shift its preferred settling and feeding site.
abstract: BACKGROUND
  The whitefly Bemisia tabaci (Genn.) causes dramatic damage to plants by transmitting yield-limiting virus diseases. Previous studies proved that the tomato breeding line ABL 14-8 was resistant to B. tabaci, the vector of tomato yellow leaf curl disease (TYLCD). This resistance is based on the presence of type IV glandular trichomes and acylsucrose production. These trichomes deter settling and probing of B. tabaci in ABL 14-8, which reduces primary and secondary spread of TYLCD.
  
  
  RESULTS
  Whitefly settlement preference was evaluated on the adaxial and abaxial leaf surfaces of nearly-isogenic tomato lines with and without B. tabaci-resistance traits, 'ABL 14-8 and Moneymaker' respectively, under non-choice and free-choice conditions. In addition, the Electrical Penetration Graph technique was used to study probing and feeding activities of B. tabaci on the adaxial and abaxial leaf surfaces of the same genotypes. B. tabaci preferred to settle on the abaxial than on the adaxial surface of 'Moneymaker' leaves, whereas no such preference was observed on ABL 14-8 tomato plants at the ten-leaf growth stage. Furthermore, B. tabaci preferred to feed on the abaxial than on the adaxial leaf surface of 'Moneymarker' susceptible tomato plants as shown by a higher number of sustained phloem feeding ingestion events and a shorter time to reach the phloem. However, B. tabaci standard probing and feeding behavior patterns were altered in ABL 14-8 plants and whiteflies were unable to feed from the phloem and spent more time in non-probing activities when exposed to the abaxial leaf surface.
  
  
  CONCLUSIONS
  The distorted behavior of B. tabaci on ABL 14-8 protects tomato plants from the transmission of phloem-restricted viruses such as Tomato yellow leaf curl virus (TYLCV), and forces whiteflies to feed on the adaxial side of leaves where they feed less efficiently and become more vulnerable to natural enemies.
authors.affiliation: Instituto de Ciencias Agrarias (ICA), Consejo Superior de Investigaciones Científicas, Madrid, Spain.
authors.forename: Maria Jose
authors.initials: MJ
authors.lastname: Rodríguez-López
authors.forename: Elisa
authors.initials: E
authors.lastname: Garzo
authors.forename: Jean Patrick
authors.initials: JP
authors.lastname: Bonani
authors.forename: Rafael
authors.initials: R
authors.lastname: Fernández-Muñoz
authors.forename: Enrique
authors.initials: E
authors.lastname: Moriones
authors.forename: Alberto
authors.initials: A
authors.lastname: Fereres
mesh_terms: D000818:Animals; D002755:Choice Behavior / Q000502:physiology; D060467:Disease Resistance / Q000502:physiology*; D005247:Feeding Behavior / Q000502:physiology*; D006430:Hemiptera / Q000502:physiology* / Q000821:virology; D007303:Insect Vectors / Q000502:physiology* / Q000821:virology; D018551:Solanum lycopersicum / Q000378:metabolism*; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000737:chemistry; D013045:Species Specificity; D013395:Sucrose / Q000378:metabolism*
chemical_list: D013395:Sucrose