title: Interaction between relaxase MbeA and accessory protein MbeC of the conjugally mobilizable plasmid ColE1.
abstract: MbeA and MbeC are two key proteins in plasmid ColE1 conjugal mobilization. Isothermal titration calorimetry was used to detect and quantify an interaction between MbeA and MbeC. As a result of this interaction, the affinity of MbeA for single stranded DNA increased. The interaction was confirmed in vivo using a bacterial two-hybrid system, which revealed that MbeA-MbeC complexes are formed through the amino-terminal region of MbeA and the carboxy-terminal region of MbeC. To the best of our knowledge, this is the first report of direct interactions between conjugative proteins encoded by a mobilizable plasmid.
authors.affiliation: Sector of Organic Chemistry and Biochemistry, Department of Chemistry, University of Ioannina, Ioannina, Greece. avarsaki@gmail.com
authors.forename: Athanasia
authors.initials: A
authors.lastname: Varsaki
authors.forename: Heather K
authors.initials: HK
authors.lastname: Lamb
authors.forename: Olga
authors.initials: O
authors.lastname: Eleftheriadou
authors.forename: Elpiniki
authors.initials: E
authors.lastname: Vandera
authors.forename: Paul
authors.initials: P
authors.lastname: Thompson
authors.forename: Gabriel
authors.initials: G
authors.lastname: Moncalián
authors.forename: Fernando
authors.initials: F
authors.lastname: de la Cruz
authors.forename: Alastair R
authors.initials: AR
authors.lastname: Hawkins
authors.forename: Constantin
authors.initials: C
authors.lastname: Drainas
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D003227:Conjugation, Genetic; D004277:DNA, Single-Stranded / Q000378:metabolism; D004706:Endodeoxyribonucleases / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
chemical_list: D001426:Bacterial Proteins; D004277:DNA, Single-Stranded; C042833:ROM protein, Bacteria; D004706:Endodeoxyribonucleases