title: Development of multiplex real-time quantitative PCR for simultaneous detection of Chlamydia trachomatis and Ureaplasma parvum.
abstract: OBJECTIVE
  Chlamydia trachomatis and Ureaplasma urealyticum are common pathogens of sexually transmitted diseases. The majority of human ureaplasma isolates belong to the new species U. parvum. Clinically, C. trachomatis and U. parvum usually double infect in the nongonococcal urethritis patients. A novel method for simultaneous detection of C. trachomatis and U. parvum was set up in the present work.
  
  
  METHODS
  Multiple real-time quantitative PCR was developed to allow for rapid, sensitive, specific and quantitative detection of C. trachomatis and U. parvum, simultaneously. To evaluate the applicability of the multiplex real-time quantitative PCR assay to clinical specimens, 64 samples of cervical swabs collected were studied.
  
  
  RESULTS
  Compared to the results obtained from single real-time quantitative PCR of C. trachomatis and U. parvum, the specificity, sensitivity and quantitative detection results of multiple real-time quantitative PCR are approximately identical with those of the former.
  
  
  CONCLUSIONS
  This assay will be of great value in the simultaneous and rapid diagnosis of C. trachomatis and U. parvum in the future.
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Hong-bo
authors.initials: HB
authors.lastname: Wei
authors.forename: She-xiao
authors.initials: SX
authors.lastname: Zou
authors.forename: Xiao-lin
authors.initials: XL
authors.lastname: Yang
authors.forename: Dai-qin
authors.initials: DQ
authors.lastname: Yang
authors.forename: Xiang-dong
authors.initials: XD
authors.lastname: Chen
mesh_terms: D000328:Adult; D002138:Calibration; D002690:Chlamydia Infections / Q000175:diagnosis* / Q000382:microbiology; D002692:Chlamydia trachomatis / Q000235:genetics* / Q000302:isolation & purification; D003001:Cloning, Molecular; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis*; D005260:Female; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D010957:Plasmids; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D014509:Ureaplasma / Q000235:genetics* / Q000302:isolation & purification; D016869:Ureaplasma Infections / Q000175:diagnosis* / Q000382:microbiology; D014526:Urethritis / Q000150:complications / Q000175:diagnosis* / Q000382:microbiology
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial