title: Quantitation of slow release triptorelin in beagle dog plasma by liquid chromatography-tandem mass spectrometry.
abstract: A sensitive method based on liquid chromatography-tandem mass spectrometry has been developed for the determination of triptorelin levels in beagle dog plasma. Plasma samples were applied to Oasis(®) HLB solid-phase extraction (SPE) cartridges. Extracted samples were evaporated under a stream of nitrogen and then reconstituted with 100 μl methanol:water:formic acid (60:40:0.08, v/v/v). The separation was achieved on a Venusil MP-C18 column (2.1 mm × 50 mm, 3 μm, Agela) with a gradient elution. Detection utilized a Qtrap5500 system operated in the positive ion mode with multiple reaction monitoring of the analyte at m/z 656.5→249.1 and of the I.S. at m/z 510.8→120.1. The proposed method was validated by assessing the specificity, linearity, precision and accuracy, recovery, matrix effects, and stability. Linear calibration curves were obtained in the concentration range of 0.01-10 ng/ml (the correlation coefficients were above 0.995). The lower limit of quantification (LLOQ) of the method was 0.01 ng/ml. The method was successfully applied to a pharmacokinetic study of a slow release triptorelin formulation in beagle dogs following a single intramuscular injection.
authors.affiliation: State Key Laboratory of Long-acting and Targeting Drug Delivery System, Shandong Luye Pharmaceutical Co. Ltd, Yantai 264003, PR China.
authors.forename: Jiangbin
authors.initials: J
authors.lastname: Han
authors.forename: Jiye
authors.initials: J
authors.lastname: Sun
authors.forename: Chunjie
authors.initials: C
authors.lastname: Sha
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Zhang
authors.forename: Yunyun
authors.initials: Y
authors.lastname: Gai
authors.forename: Youxin
authors.initials: Y
authors.lastname: Li
authors.forename: Wanhui
authors.initials: W
authors.lastname: Liu
mesh_terms: D000818:Animals; D018931:Antineoplastic Agents, Hormonal / Q000008:administration & dosage / Q000493:pharmacokinetics*; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D003692:Delayed-Action Preparations; D004285:Dogs; D007273:Injections, Intramuscular; D057230:Limit of Detection; D008297:Male; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry / Q000379:methods*; D017329:Triptorelin Pamoate / Q000008:administration & dosage / Q000493:pharmacokinetics*
chemical_list: D018931:Antineoplastic Agents, Hormonal; D003692:Delayed-Action Preparations; D017329:Triptorelin Pamoate