title: [Nutritional components and sub-chronic toxicity of genetically modified rice expressing human lactoferrin].
abstract: OBJECTIVE
  To compare the nutritional components of genetically modified rice expressing human lactoferrin (hLf) with its parental rice, and to observe the sub-chronic toxicity of hLf rice.
  
  
  METHODS
  The nutritional components of hLf rice and its parental rice were determined by the National Standard Methods. Eighty weanling Wistar rats were randomly divided into 4 groups based on their gender and body weight: group A (hLf rice high-dose group with 71.45% rice), group B (hLf rice medium-dose group with 35. 725% rice), group C (parental rice group with 71.01% rice) and group D (AIN-93G diet group), and the latter two groups were used as the control. Body weight, dietary intake, blood routine test, blood biochemical examination, organ coefficient, bone density and the pathology of organs were investigated at the end of a 90-day feeding experiment.
  
  
  RESULTS
  Except for human lactoferrin and Fe, there was no difference of main nutritional components, minerals and vitamins between groups. The differences of some indicators of blood routine (WBC, HGB, RBC and MCH), blood biochemistry (AST and GLU), organ coefficient and bone density between group A and B (hLf rice) with group C (parental rice) or group D (AIN-93G) were significant, while no difference of other indicators.
  
  
  CONCLUSIONS
  Although some differences were observed, all indicators were still in the normal reference range. Therefore, there was no sign of toxic and adverse effects for hLf rice on rats.
authors.affiliation: Institute of Nutrition and Food Safety, China CDC, Beijing 100050, China. huyichun0319@163.com
authors.forename: Yichun
authors.initials: Y
authors.lastname: Hu
authors.forename: Jianhua
authors.initials: J
authors.lastname: Piao
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Yang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D005260:Female; D030841:Food, Genetically Modified / Q000633:toxicity*; D006801:Humans; D007781:Lactoferrin / Q000235:genetics*; D008297:Male; D012275:Oryza / Q000737:chemistry* / Q000235:genetics / Q000633:toxicity*; D010446:Peptide Fragments / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D059550:Toxicity Tests, Subchronic
chemical_list: C112552:HLP1 protein, synthetic; D010446:Peptide Fragments; D007781:Lactoferrin