title: Immunogenicity of a multi-epitope DNA vaccine against hantavirus.
abstract: Hemorrhagic fever with renal syndrome (HFRS) is a severe epidemic disease caused by hantaviruses including Hantaan virus (HTNV), Seoul virus (SEOV), Dobrava virus (DOBV) and Puumala virus. Three of the four HFRS hantaviruses, HTNV, SEOV, and PUUV are found in China. Currently, there is no effective strategy available to reduce infection risk. In this study, we constructed a multi-epitope chimeric DNA vaccine that encodes expressing 25 glycoprotein epitopes from SEOV, HTNV and PUUV (designated as SHP chimeric gene). Vaccination of BALb/c mice with SHP multi-epitope chimeric DNA vaccine led to a dramatic augmentation of humoral and cellular responses. The SHP vaccine DNA was detected in many organs but not for more than 60 d. There was no risk of mutation due to integration. Thus, the SHP multi-epitope chimeric DNA vaccine is a potential effective and safe DNA vaccine against infection by SEOV, HTNV, and PUUV.
authors.affiliation: China Medical University, Shenyang, Liaoning, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Zhao
authors.forename: Ying
authors.initials: Y
authors.lastname: Sun
authors.forename: Yujie
authors.initials: Y
authors.lastname: Zhao
authors.forename: Si
authors.initials: S
authors.lastname: Wang
authors.forename: Tongtong
authors.initials: T
authors.lastname: Yu
authors.forename: Feng
authors.initials: F
authors.lastname: Du
authors.forename: X Frank
authors.initials: XF
authors.lastname: Yang
authors.forename: Enjie
authors.initials: E
authors.lastname: Luo
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology; D002478:Cells, Cultured; D016207:Cytokines / Q000097:blood*; D000939:Epitopes / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology; D018063:Hantaan virus / Q000276:immunology*; D006480:Hemorrhagic Fever with Renal Syndrome / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D007371:Interferon-gamma / Q000097:blood; D016753:Interleukin-10 / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D029262:Puumala virus / Q000276:immunology*; D011897:Random Allocation; D029263:Seoul virus / Q000276:immunology*; D019444:Vaccines, DNA; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D016207:Cytokines; D000939:Epitopes; D006023:Glycoproteins; D019444:Vaccines, DNA; D014765:Viral Vaccines; D016753:Interleukin-10; D015847:Interleukin-4; D007371:Interferon-gamma