title: [Anti-tumor effect of adenovirus-mediated Bcl-XL shRNA in vitro].
abstract: OBJECTIVE
  To investigate the anti-tumor effect of adenovirus-mediated Bcl-XL shRNA on colon cancer cells in vitro.
  
  
  METHODS
  A recombinant Bcl-xl adenovirus was constructed, amplified, and purified. The effect on mRNA expression of Bcl-XL was assessed by RT-PCR, and the effect on apoptosis-induction of colon cancer(Lovo cell line) in vitro was assessed by MTT assay and cell clonogenic assay.
  
  
  RESULTS
  RT-PCR showed that Ad/Bcl-XL shRNA significantly down-regulated the mRNA expression of Bcl-XL in Lovo cells. Ad/Bcl-XL shRNA suppressed the proliferation of Lovo cells in a dose-dependent as well as a time-dependent manner compared with Ad/GFP (P<0.05). Treatment with Ad/Bcl-XL shRNA dramatically suppressed the colony formation of Lovo cells in a dose-dependent manner (P<0.05). Ad/Bcl-XL shRNA showed no effect on normal human fibroblast.
  
  
  CONCLUSIONS
  Ad/Bcl-XL shRNA exhibits cytotoxic effect on Lovo cells and may have the potential value in the treatment of colon cancer.
authors.affiliation: Department of Colorectal Surgery, Zhejiang Cancer Hospital, Hangzhou 310022, China.
authors.forename: Yu-ping
authors.initials: YP
authors.lastname: Zhu
authors.forename: De-chuan
authors.initials: DC
authors.lastname: Li
authors.forename: Hai-yang
authors.initials: HY
authors.lastname: Feng
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Qian
authors.forename: Yin-bo
authors.initials: YB
authors.lastname: Chen
authors.forename: Yun
authors.initials: Y
authors.lastname: Gao
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D034741:RNA, Small Interfering / Q000235:genetics*; D051020:bcl-X Protein / Q000235:genetics* / Q000378:metabolism
chemical_list: D034741:RNA, Small Interfering; D051020:bcl-X Protein