title: Kinetic schemes for post-synchronized single molecule dynamics.
abstract: Recordings from single molecule experiments can be aggregated to determine average kinetic properties of the system under observation. The kinetics after a synchronized reaction step can be interpreted using all of the standard tools developed for ensemble perturbation experiments. The kinetics leading up to a synchronized event, determined by the lifetimes of the preceding states; however, are not as obvious if the reaction has reversible steps or branches. Here we describe a general procedure for dealing with these situations.
authors.affiliation: Pennsylvania Muscle Institute and Department of Physiology, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania, USA.
authors.forename: Chunlai
authors.initials: C
authors.lastname: Chen
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Greenberg
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Laakso
authors.forename: E Michael
authors.initials: EM
authors.lastname: Ostap
authors.forename: Yale E
authors.initials: YE
authors.lastname: Goldman
authors.forename: Henry
authors.initials: H
authors.lastname: Shuman
mesh_terms: D000205:Actomyosin / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer; D007700:Kinetics; D056004:Molecular Dynamics Simulation; D052898:Optical Tweezers; D020653:Peptide Elongation Factor G / Q000378:metabolism; D014176:Protein Biosynthesis
chemical_list: D020653:Peptide Elongation Factor G; D000205:Actomyosin