title: Identification and characterization of genes related to the development of breast muscles in Pekin duck.
abstract: Pekin Duck is world-famous for its fast growth, but its breast muscle development is later and breast muscle content is lower compared with other muscular ducks. Therefore, it is very important to discover the genetic mechanism between breast muscle development and relative gene expression in Pekin duck. In current study, the genes which have relationships with breast muscle development were identified by suppression subtractive hybridization. A total of 403 positive clones were sequenced and 257 unigenes were obtained. The expression of 23 genes were analyzed in the breast muscle of 2-, 4-, 6-, 8- week old Pekin ducks. The results showed that unknown clone A233, C83 and C99 showed descending tendency as age increased; KBTBD10, HSPA8, MYL1, ZFP622, MARCH4, Nexilin, FABP4 and MUSTN1 had high expression levels at 6 weeks old; WAC, NT5C3, HSP90AA1, MRPL33, KLF6, TSNAX, CDC42EP3, HSPA4, TRAK1, NR2F2, HAUS1 and IGF1 had high expression levels at 8 weeks and showed ascending tendency as age increased. Expression of these 23 genes were also analyzed in breast muscle, leg muscle, heart, kidney, liver, muscular stomach and sebum cutaneum in 4-8-week old Pekin duck and results showed that most of these genes had high expression in breast muscle, leg muscle and heart.
authors.affiliation: State Key Laboratory of Animal Nutrition Science, Institute of Animal Science, CAAS, No 2, YuanMingYuan West Road, HaiDian, 100193 Beijing, People's Republic of China.
authors.forename: Tieshan
authors.initials: T
authors.lastname: Xu
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Zhang
authors.forename: Baoguo
authors.initials: B
authors.lastname: Ye
authors.forename: Hanlin
authors.initials: H
authors.lastname: Zhou
authors.forename: Shuisheng
authors.initials: S
authors.lastname: Hou
mesh_terms: D000818:Animals; D001483:Base Sequence; D004372:Ducks / Q000235:genetics* / Q000254:growth & development / Q000502:physiology*; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D005796:Genes; D008297:Male; D008969:Molecular Sequence Data; D024510:Muscle Development / Q000235:genetics*; D018482:Muscle, Skeletal / Q000254:growth & development*; D017422:Sequence Analysis, DNA; D013909:Thorax / Q000254:growth & development