title: Miniaturized protein microarray with internal calibration as point-of-care device for diagnosis of neonatal sepsis.
abstract: Neonatal sepsis is still a leading cause of death among newborns. Therefore a protein-microarray for point-of-care testing that simultaneously quantifies the sepsis associated serum proteins IL-6, IL-8, IL-10, TNF alpha, S-100, PCT, E-Selectin, CRP and Neopterin has been developed. The chip works with only a 4 μL patient serum sample and hence minimizes excessive blood withdrawal from newborns. The 4 μL patient samples are diluted with 36 μL assay buffer and distributed to four slides for repetitive measurements. Streptavidin coated magnetic particles that act as distinct stirring detection components are added, not only to stir the sample, but also to detect antibody antigen binding events. We demonstrate that the test is complete within 2.5 h using a single step assay. S-100 conjugated to BSA is spotted in increasing concentrations to create an internal calibration. The presented low volume protein-chip fulfills the requirements of point-of-care testing for accurate and repeatable (CV < 14%) quantification of serum proteins for the diagnosis of neonatal sepsis.
authors.affiliation: Health & Environment Department, AIT Austrian Institute of Technology, Bioresources, Konrad Lorenz Strasse 24, 3430 Tulln, Austria. patricia.buchegger.fl@ait.ac.at
authors.forename: Patricia
authors.initials: P
authors.lastname: Buchegger
authors.forename: Ursula
authors.initials: U
authors.lastname: Sauer
authors.forename: Hedvig
authors.initials: H
authors.lastname: Toth-Székély
authors.forename: Claudia
authors.initials: C
authors.lastname: Preininger
mesh_terms: D015415:Biomarkers / Q000097:blood; D015374:Biosensing Techniques / Q000295:instrumentation*; D002138:Calibration; D016207:Cytokines / Q000097:blood*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D007231:Infant, Newborn; D008904:Miniaturization; D019095:Point-of-Care Systems; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000592:standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D018805:Sepsis / Q000097:blood* / Q000175:diagnosis*
chemical_list: D015415:Biomarkers; D016207:Cytokines