title: [Effect of STAT5 gene silencing on the proliferation of T lymphocytes in a mouse model of asthma].
abstract: OBJECTIVE
  To study the effect of silencing STAT5 gene on the proliferation of T lymphocytes in a mouse model of asthma.
  
  
  METHODS
  Spleen T-lymphocytes of normal and asthmatic mice were selected by immunomagnetic beads, and the STAT5a/b genes of these T-lymphocytes were silenced by siRNA. The mRNA of STAT5a/b was detected by fluorescence quantitative PCR, and the protein by Western blot. The proliferation rates of T-lymphocytes was evaluated by CCK-8, and the cell cycle and the cell apoptosis was measured by flow cytometry.
  
  
  RESULTS
  (1) Significant inflammatory cell infiltration was present in asthmatic mouse airways, as compared to the normal control mice. (2) The STAT5a/b mRNA expression of the asthmatic group was significantly higher compared with the normal control group. (3) Compared to the blank control group, the mRNA expression of STAT5a/b was reduced in the siRNA-specific intervention (T: 35.014 vs 14.553, P < 0.01). The protein expression of STAT5a/b was also reduced after siRNA-specific intervention (T: -10.958 vs -14.706, P < 0.01). The proliferation rates of T lymphocytes was reduced after siRNA-specific intervention (T: 8.692 vs 10.540, P < 0.01), and the number of T-lymphocytes in proliferative phase was all significantly reduced after siRNA-specific intervention (T: 6.975 vs -5.567, P < 0.05). The apoptosis rates of T lymphocytes were significantly increased after siRNA-specific intervention (T: -6.404 vs -6.038, P < 0.05).
  
  
  CONCLUSIONS
  RNA interference specifically reduced the expression of STAT5a/b, inhibited the proliferation of T lymphocytes, and promoted the apoptosis of T lymphocytes in a mouse model of asthma.
authors.affiliation: Department of Respiratory Medicine, Second Clinical Medical College, Jinan University, Shenzhen 518020, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Qiu
authors.forename: Ting
authors.initials: T
authors.lastname: Zhang
authors.forename: Hui
authors.initials: H
authors.lastname: Qi
authors.forename: Wen-ke
authors.initials: WK
authors.lastname: Peng
authors.forename: Fei
authors.initials: F
authors.lastname: Shi
mesh_terms: D000818:Animals; D001249:Asthma / Q000235:genetics* / Q000378:metabolism; D002453:Cell Cycle; D049109:Cell Proliferation; D004195:Disease Models, Animal; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D034622:RNA Interference; D034741:RNA, Small Interfering; D050799:STAT5 Transcription Factor / Q000235:genetics*; D013601:T-Lymphocytes / Q000166:cytology* / Q000378:metabolism
chemical_list: D034741:RNA, Small Interfering; D050799:STAT5 Transcription Factor