title: Engineering humanized mice for improved hematopoietic reconstitution.
abstract: Humanized mice are immunodeficient animals engrafted with human hematopoietic stem cells that give rise to various lineages of human blood cells throughout the life of the mouse. This article reviews recent advances in the generation of humanized mice, focusing on practical considerations. We discuss features of different immunodeficient recipient mouse strains, sources of human hematopoietic stem cells, advances in expansion and genetic modification of hematopoietic stem cells, and techniques to modulate the cytokine environment of recipient mice, in order to enhance reconstitution of specific human blood lineage cells. We highlight the opportunities created by new technologies and discuss practical considerations on how to make best use of the widening array of basic models for specific research applications.
authors.affiliation: Koch Institute for Integrative Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.
authors.forename: Adam C
authors.initials: AC
authors.lastname: Drake
authors.forename: Qingfeng
authors.initials: Q
authors.lastname: Chen
authors.forename: Jianzhu
authors.initials: J
authors.lastname: Chen
mesh_terms: D000818:Animals; D002470:Cell Survival; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D005818:Genetic Engineering; D006410:Hematopoiesis / Q000235:genetics / Q000276:immunology; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D056747:Immunomodulation; D051379:Mice; D008817:Mice, Mutant Strains; D016513:Mice, SCID; D012107:Research Design; D055153:Stem Cell Niche; D014183:Transplantation, Heterologous
chemical_list: D016207:Cytokines