title: Inverse production of IL-6 and IL-10 by abdominal aortic aneurysm explant tissues in culture.
abstract: BACKGROUND
  Abdominal aortic aneurysm is considered an atherosclerosis-related disease, but the mechanisms underlying abdominal aortic aneurysm remain poorly defined. Despite the large number of cytokines identified in an aneurysm sample, the relative importance of particular cytokines in aneurysm formation is unknown. We have studied the production of interleukin-6 and interleukin-10 cytokines in plasma and cultures of abdominal aortic aneurysm explant samples obtained from patients subjected to elective surgery and their correlation with cellular composition.
  
  
  METHODS
  Inflammatory cells from the abdominal aortic aneurysm samples were phenotypically characterized using specific monoclonal antibodies (anti-CD3, -CD4, -CD8, -CD19, -CD38, -CD68, -HLA-DR) by means of immunocytochemistry staining. Production of interleukin-6 and interleukin-10 in culture supernatants of abdominal aortic aneurysm explant samples expanded in vitro for 24 h was measured by enzyme-linked immunosorbent assay.
  
  
  RESULTS
  We showed that the levels of interleukin-6 and interleukin-10 in supernatants of abdominal aortic aneurysm sample cultures were higher by 73 and 86 times compared to their levels in plasma, respectively. In individual abdominal aortic aneurysm explant cultures, a negative correlation between interleukin-6 and interleukin-10 production was observed. Such inverse correlation was not detected in plasma. Based on these results, we divided abdominal aortic aneurysm into two cytokine-producing groups and showed that the interleukin-6(hi)/interleukin-10(lo) group contained higher percentages of granulocytes, HLA-DR(+), and CD68(+) cells but lower percentages of lymphocytes and plasma cells compared to the interleukin-6(lo)/interleukin-10(hi) group. Exogenously added interleukin-10 suppresses the production of interleukin-6 by abdominal aortic aneurysm explants.
  
  
  CONCLUSIONS
  These results suggest that interleukin-6 and interleukin-10 may have a different role in the pathogenesis of abdominal aortic aneurysm.
authors.affiliation: Institute of Medical Research, Military Medical Academy, Belgrade, Serbia. vucevic122@sbb.rs
authors.forename: Dragana
authors.initials: D
authors.lastname: Vucevic
authors.forename: Vera
authors.initials: V
authors.lastname: Maravic-Stojkovic
authors.forename: Sasa
authors.initials: S
authors.lastname: Vasilijic
authors.forename: Milica
authors.initials: M
authors.lastname: Borovic-Labudovic
authors.forename: Ivana
authors.initials: I
authors.lastname: Majstorovic
authors.forename: Djordje
authors.initials: D
authors.lastname: Radak
authors.forename: Miodrag
authors.initials: M
authors.lastname: Jevtic
authors.forename: Petar
authors.initials: P
authors.lastname: Milosavljevic
authors.forename: Miodrag
authors.initials: M
authors.lastname: Colic
mesh_terms: D000368:Aged; D001012:Aorta, Abdominal / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D017544:Aortic Aneurysm, Abdominal / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D015415:Biomarkers; D017077:Culture Media, Conditioned / Q000737:chemistry; D005260:Female; D006098:Granulocytes / Q000378:metabolism / Q000473:pathology; D006801:Humans; D016130:Immunophenotyping; D016753:Interleukin-10 / Q000378:metabolism* / Q000494:pharmacology; D015850:Interleukin-6 / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009924:Organ Culture Techniques; D010950:Plasma Cells / Q000378:metabolism / Q000473:pathology
chemical_list: D015415:Biomarkers; D017077:Culture Media, Conditioned; C508609:IL10 protein, human; C508600:IL6 protein, human; D015850:Interleukin-6; D016753:Interleukin-10