title: Chemiluminescence enzyme immunoassay using magnetic nanoparticles for detection of neuron specific enolase in human serum.
abstract: To detect a biomarker for small cell lung carcinoma, neuron specific enolase (NSE), a sensitive and specific chemiluminescence enzyme immunoassay was developed. Fluorescein isothiocyanate (FITC) labeled NSE capture antibody connected with NSE and alkaline phosphatase (ALP) labeled NSE detection antibody in a sandwich-type detection manner. This immune complex was further reacted with anti-FITC coated magnetic beads. In a magnetic field, the complex was enriched, and the sensitivity was thus enhanced. The limit of detection (LOD) of this method was <0.2 ng mL(-1). The proposed immunoassay was highly selective, and not interfered by hook effect. The recovery was >83.0% and the coefficient of variation was <10.0%. Human sera from 120 patients were tested with the presented and traditional chemiluminescence enzyme immunoassay. An excellent linear relationship was obtained between two techniques. Overall, this immunoassay offers a promising alternative for NSE detection than traditional clinical examinations.
authors.affiliation: Department of Orthopaedics, The Second Xiangya Hospital, Central South University, Changsha, 410011, China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Fu
authors.forename: Meng
authors.initials: M
authors.lastname: Meng
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yongmei
authors.initials: Y
authors.lastname: Yin
authors.forename: Xiangsheng
authors.initials: X
authors.lastname: Zhang
authors.forename: Rimo
authors.initials: R
authors.lastname: Xi
mesh_terms: D000469:Alkaline Phosphatase / Q000276:immunology / Q000378:metabolism; D055910:Antibodies, Immobilized / Q000276:immunology; D014408:Biomarkers, Tumor / Q000097:blood; D004800:Enzymes, Immobilized / Q000378:metabolism; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D006801:Humans; D007124:Immunoenzyme Techniques; D008163:Luminescent Measurements; D008175:Lung Neoplasms / Q000175:diagnosis; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D010751:Phosphopyruvate Hydratase / Q000097:blood* / Q000276:immunology
chemical_list: D055910:Antibodies, Immobilized; D014408:Biomarkers, Tumor; D004800:Enzymes, Immobilized; D058185:Magnetite Nanoparticles; D000469:Alkaline Phosphatase; D010751:Phosphopyruvate Hydratase; D016650:Fluorescein-5-isothiocyanate