title: Immuno-Golgi as a tool for analyzing neuronal 3D-dendritic structure in phenotypically characterized neurons.
abstract: Characterization of neuronal dendritic structure in combination with the determination of specific neuronal phenotype or temporal generation is a challenging task. Here we present a novel method that combines bromodioxyuridine (BrdU) immunohistochemistry with Golgi-impregnation technique; with this simple non-invasive method, we are able to determine the tridimensional structure of dendritic arborization and spine shape of neurons born at a specific time in the hippocampus of adult animals. This analysis is relevant in physiological and pathological conditions in which altered neurogenesis is implicated, such as aging or emotional disorders.
authors.affiliation: School of Health Sciences, Life and Health Sciences Research Institute, University of Minho, Braga, Portugal. luisapinto@ecsaude.uminho.pt
authors.forename: Luísa
authors.initials: L
authors.lastname: Pinto
authors.forename: António
authors.initials: A
authors.lastname: Mateus-Pinheiro
authors.forename: Mónica
authors.initials: M
authors.lastname: Morais
authors.forename: João Miguel
authors.initials: JM
authors.lastname: Bessa
authors.forename: Nuno
authors.initials: N
authors.lastname: Sousa
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001973:Bromodeoxyuridine; D004396:Coloring Agents; D003712:Dendrites / Q000648:ultrastructure*; D006624:Hippocampus / Q000166:cytology*; D007150:Immunohistochemistry / Q000379:methods*; D008297:Male; D018613:Microscopy, Confocal; D009474:Neurons / Q000166:cytology*; D010641:Phenotype; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004396:Coloring Agents; D001973:Bromodeoxyuridine