title: Quantitative proteome profiling of C. burnetii under tetracycline stress conditions.
abstract: The recommended antibiotic regimen against Coxiella burnetii, the etiological agent of Q fever, is based on a semi-synthetic, second-generation tetracycline, doxycycline. Here, we report on the comparison of the proteomes of a C. burnetii reference strain either cultured under control conditions or under tetracycline stress conditions. Using the MS-driven combined fractional diagonal chromatography proteomics technique, out of the 531 proteins identified, 5 and 19 proteins were found significantly up- and down-regulated respectively, under tetracycline stress. Although the predicted cellular functions of these regulated proteins did not point to known tetracycline resistance mechanisms, our data clearly reveal the plasticity of the proteome of C. burnetii to battle tetracycline stress. Finally, we raise several plausible hypotheses that could further lead to more focused experiments on studying tetracycline resistance in C. burnetii and thus reduced treatment failures of Q fever.
authors.affiliation: Department of Clinical Bacteriology, Parasitology, Zoonoses, and Geographical Medicine, Medical School, University of Crete, Heraklion, Greece. iosif.vranakis@gmail.com
authors.forename: Iosif
authors.initials: I
authors.lastname: Vranakis
authors.forename: Pieter-Jan
authors.initials: PJ
authors.lastname: De Bock
authors.forename: Anastasia
authors.initials: A
authors.lastname: Papadioti
authors.forename: Yannis
authors.initials: Y
authors.lastname: Tselentis
authors.forename: Kris
authors.initials: K
authors.lastname: Gevaert
authors.forename: Georgios
authors.initials: G
authors.lastname: Tsiotis
authors.forename: Anna
authors.initials: A
authors.lastname: Psaroulaki
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D016997:Coxiella burnetii / Q000187:drug effects* / Q000378:metabolism*; D015536:Down-Regulation / Q000187:drug effects; D024881:Drug Resistance, Bacterial; D006801:Humans; D040081:Protein Array Analysis; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D011778:Q Fever / Q000188:drug therapy / Q000382:microbiology; D013312:Stress, Physiological; D013752:Tetracycline / Q000494:pharmacology*; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D001426:Bacterial Proteins; D020543:Proteome; D013752:Tetracycline