title: [Application of DNA extraction kit, 'GM quicker' for detection of genetically modified soybeans].
abstract: Several DNA extraction methods have been officially introduced to detect genetically modified soybeans, but the choice of DNA extraction kits depend on the nature of the samples, such as grains or processed foods. To overcome this disadvantage, we examined whether the GM quicker kit is available for both grains and processed foods. We compared GM quicker with four approved DNA extraction kits in respect of DNA purity, copy numbers of lectin gene, and working time. We found that the DNA quality of GM quicker was superior to that of the other kits for grains, and the procedure was faster. However, in the case of processed foods, GM quicker was not superior to the other kits. We therefore investigated an unapproved GM quicker 3 kit, which is available for DNA extraction from processed foods, such as tofu and boiled soybeans. The GM quicker 3 kit provided good DNA quality from both grains and processed foods, so we made a minor modification of the GM quicker-based protocol that was suitable for processed foods, using GM quicker and its reagents. The modified method enhanced the performance of GM quicker with processed foods. We believe that GM quicker with the modified protocol is an excellent tool to obtain high-quality DNA from grains and processed foods for detection of genetically modified soybeans.
authors.affiliation: Department of Food Chemistry, Kobe Institute of Health,Kobe, Japan. noriko_sato@office.city.kobe.lg.jp
authors.forename: Noriko
authors.initials: N
authors.lastname: Sato
authors.forename: Yoshitsugu
authors.initials: Y
authors.lastname: Sugiura
authors.forename: Toshitsugu
authors.initials: T
authors.lastname: Tanaka
mesh_terms: D018744:DNA, Plant / Q000032:analysis*; D030841:Food, Genetically Modified; D005821:Genetic Techniques / Q000295:instrumentation; D030821:Plants, Genetically Modified / Q000235:genetics; D016133:Polymerase Chain Reaction; D013025:Glycine max / Q000235:genetics*
chemical_list: D018744:DNA, Plant