title: Neurons and stromal stem cells as targets for polycation-mediated transfection.
abstract: Expression of transgenes in neurons and stromal/mesenchymal stem cells (MSC) can greatly enhance their therapeutic potential. In transfection experiments, we studied properties of linear and branched (dendrimers) polycations as transgene delivery vehicles. Linear polyethyleneimine transfected neurons, but was ineffective in MSC. Polyamidoamine dendrimers showed greater transfection efficiency and mean GFP fluorescence intensity compared to phosphorus dendrimers of the same (4th) generation. Expression of neurotrophic factor BDNF in MSC transfected with polyamidoamine dendrimers was also by more than 10 times higher.
authors.affiliation: Department of Clinical Neurobiology, University of Calgary, Canada. shakhbazau@gmail.com
authors.forename: A V
authors.initials: AV
authors.lastname: Shakhbazau
authors.forename: D G
authors.initials: DG
authors.lastname: Shcharbin
authors.forename: N V
authors.initials: NV
authors.lastname: Goncharova
authors.forename: I N
authors.initials: IN
authors.lastname: Seviaryn
authors.forename: S M
authors.initials: SM
authors.lastname: Kosmacheva
authors.forename: N A
authors.initials: NA
authors.lastname: Kartel
authors.forename: M
authors.initials: M
authors.lastname: Bryszewska
authors.forename: J P
authors.initials: JP
authors.lastname: Majoral
authors.forename: M P
authors.initials: MP
authors.lastname: Potapnev
mesh_terms: D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics / Q000378:metabolism; D050091:Dendrimers / Q000737:chemistry*; D015870:Gene Expression; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D011073:Polyamines / Q000737:chemistry*; D000071228:Polyelectrolytes; D011094:Polyethyleneimine / Q000737:chemistry; D014162:Transfection / Q000379:methods*; D019076:Transgenes
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D050091:Dendrimers; D049452:Green Fluorescent Proteins; D011073:Polyamines; D000071228:Polyelectrolytes; D011094:Polyethyleneimine