title: [Effect of M007 mediated photodynamic therapy on proliferation of human osteosarcoma MG63 cells in vitro].
abstract: OBJECTIVE
  To investigate the effect of a new photosensitizer, M007 mediated photodynamic therapy on proliferation of human osteosarcoma MG63 cells in vitro.
  
  
  METHODS
  Human osteosarcoma MG63 cells were prepared as 1 x 10(6) /mL single-cell suspension, and 1 mL cells were transferred into 60 mL culture dish, then treated with 5 different gradient dosages (0, 2, 4, 8, 16 micromol/L) of M007 followed by photodynamic therapy or dark reaction for 10 min. The survival rate of the cells and the mode of cell death were detected by flow cytometry with the stain of Annexin V-FITC/PI. The effect on proliferation of survival cells was observed by MTT assay and colony-forming assay.
  
  
  RESULTS
  M007 mediated photodynamic therapy induced the inactivation of MG63 human osteosarcoma cells in the way of late apoptosis/necrosis or becoming naked nucleus predominately. More than 90% MG63 cells in M007-PDT group were dead under the treatment of 2-16 micromol/L M007. The survival rates of 4-16 micromol/L M007-PDT group were steadily less than 1%. The optical densities did not increase with extension of culture time in 2-8 micromol/L M007-PDT group (P > 0.05). There were 16 survival alive cells found occasionally in 2 micromol/L M007-PDT group, but no colonies found in other groups.
  
  
  CONCLUSIONS
  M007 mediated photodynamic therapy totally inactivated human osteosarcoma MG63 cells in vitro with the dosage more than 4 micromol/L.
authors.affiliation: Department of Anesthesia, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Yu-Kai
authors.initials: YK
authors.lastname: Zhou
authors.forename: Wen-Zhi
authors.initials: WZ
authors.lastname: Wu
authors.forename: Lan
authors.initials: L
authors.lastname: Zhang
authors.forename: Chun-Hui
authors.initials: CH
authors.lastname: Yang
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Wang
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D001859:Bone Neoplasms / Q000473:pathology*; D016923:Cell Death / Q000187:drug effects / Q000528:radiation effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects* / Q000528:radiation effects; D006801:Humans; D012516:Osteosarcoma / Q000473:pathology*; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology*
chemical_list: D017319:Photosensitizing Agents