title: The metabolic response of P. putida KT2442 producing high levels of polyhydroxyalkanoate under single- and multiple-nutrient-limited growth: highlights from a multi-level omics approach.
abstract: BACKGROUND
  Pseudomonas putida KT2442 is a natural producer of polyhydroxyalkanoates (PHAs), which can substitute petroleum-based non-renewable plastics and form the basis for the production of tailor-made biopolymers. However, despite the substantial body of work on PHA production by P. putida strains, it is not yet clear how the bacterium re-arranges its whole metabolism when it senses the limitation of nitrogen and the excess of fatty acids as carbon source, to result in a large accumulation of PHAs within the cell. In the present study we investigated the metabolic response of KT2442 using a systems biology approach to highlight the differences between single- and multiple-nutrient-limited growth in chemostat cultures.
  
  
  RESULTS
  We found that 26, 62, and 81% of the cell dry weight consist of PHA under conditions of carbon, dual, and nitrogen limitation, respectively. Under nitrogen limitation a specific PHA production rate of 0.43 (g·(g·h)-1) was obtained. The residual biomass was not constant for dual- and strict nitrogen-limiting growth, showing a different feature in comparison to other P. putida strains. Dual limitation resulted in patterns of gene expression, protein level, and metabolite concentrations that substantially differ from those observed under exclusive carbon or nitrogen limitation. The most pronounced differences were found in the energy metabolism, fatty acid metabolism, as well as stress proteins and enzymes belonging to the transport system.
  
  
  CONCLUSIONS
  This is the first study where the interrelationship between nutrient limitations and PHA synthesis has been investigated under well-controlled conditions using a system level approach. The knowledge generated will be of great assistance for the development of bioprocesses and further metabolic engineering work in this versatile organism to both enhance and diversify the industrial production of PHAs.
authors.affiliation: Helmholtz Centre for Infection Research (HZI), Braunschweig, Germany. ignacio.pobletecastro@helmholtz-hzi.de
authors.forename: Ignacio
authors.initials: I
authors.lastname: Poblete-Castro
authors.forename: Isabel F
authors.initials: IF
authors.lastname: Escapa
authors.forename: Christian
authors.initials: C
authors.lastname: Jäger
authors.forename: Jacek
authors.initials: J
authors.lastname: Puchalka
authors.forename: Carolyn Ming Chi
authors.initials: CM
authors.lastname: Lam
authors.forename: Dietmar
authors.initials: D
authors.lastname: Schomburg
authors.forename: María Auxiliadora
authors.initials: MA
authors.lastname: Prieto
authors.forename: Vítor A P
authors.initials: VA
authors.lastname: Martins dos Santos
mesh_terms: D018533:Biomass; D002244:Carbon / Q000378:metabolism; D004734:Energy Metabolism; D005227:Fatty Acids / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D055432:Metabolomics; D009584:Nitrogen / Q000378:metabolism; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis*; D040901:Proteomics; D016958:Pseudomonas putida / Q000254:growth & development / Q000378:metabolism*; D059467:Transcriptome
chemical_list: D005227:Fatty Acids; D054813:Polyhydroxyalkanoates; D002244:Carbon; D009584:Nitrogen