title: Isolation and characterization of microsatellite loci in the endangered tree Diplopanax stachyanthus (Araliaceae).
abstract: OBJECTIVE
  Microsatellite markers were developed in Diplopanax stachyanthus to investigate the population genetics of this endangered tree.
  
  
  RESULTS
  Using the Fast Isolation by AFLP of Sequences Containing repeats (FIASCO) protocol, 15 microsatellite markers were developed in D. stachyanthus and evaluated for their variability in 25 samples from a natural population. For the 11 polymorphic loci, the number of alleles ranged from two to eight, while the observed and expected heterozygosities ranged from 0.5200 to 0.7600 and 0.4200 to 0.7813, respectively. Their cross-taxa transferability was also examined in Acanthopanax gracilistylus, Tetrapanax papyrifer, Cornus controversa, and Dendrobenthamia japonica var. chinensis, and four to 15 loci proved amplifiable in these species.
  
  
  CONCLUSIONS
  These microsatellite markers could be employed to investigate the population genetics of D. stachyanthus, and may potentially be applicable to other related species.
authors.affiliation: School of Biological Science and Technology, Central South University, Changsha, People's Republic of China. csu.wangli@gmail.com
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Min
authors.initials: M
authors.lastname: Wu
authors.forename: Zhi
authors.initials: Z
authors.lastname: Wen
authors.forename: Qiu-Ping
authors.initials: QP
authors.lastname: Xia
authors.forename: Hai-Fei
authors.initials: HF
authors.lastname: Yan
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Luo
mesh_terms: D027481:Araliaceae / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics* / Q000302:isolation & purification*; D056727:Endangered Species; D056426:Genetic Loci / Q000235:genetics*; D014644:Genetic Variation; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D014197:Trees / Q000235:genetics*
chemical_list: D018744:DNA, Plant