title: [Typing and polymorphism analysis of 16 STR loci on X chromosome].
abstract: OBJECTIVE
  To develop a PCR-based X-STR kit for typing of 16 X-STR loci and investigate the polymorphisms of the X-STR markers.
  
  
  METHODS
  Sixteen STR loci (GATA 165B12, DXS101, GATA 172D05, HPRTB, DXS981, DXS8378, DXS6795, GATA 31E08, DXS6809, DXS6803, DXS9902, DXS6807, DXS7423, DXS7133, DXS6810 and DXS7132) located on X chromosome were selected. The primers for multiplex PCR were designed by Primer Premier 5.0 software and labeled by four fluorescences (FAM, HEX, TAMRA and ROX). The developed multiplex PCR system was used for investigating the polymorphisms of the X-STR markers in Han populations.
  
  
  RESULTS
  The 16-plex amplification system named IDtyper X-16 was successfully developed and validated. Among the 16 X-STR loci, DXS7133 and DXS7423 were found to be moderately polymorphic and the other 14 X-STR markers were highly polymorphic (P1C > 0.5, H > 0.5). The cumulative discrimination power in females and in males were 0.999 999 999 999 97 and 0.999 999 993 respectively in Han population. The combined power of exclusion in trios and in duos were 0.999 999 93 and 0.999990, respectively.
  
  
  CONCLUSIONS
  The IDtyper X-16 kit is highly valuable in forensic science and is suitable for paternity testing in disputed cases.
authors.affiliation: Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, Shanghai 200063, PR China. anneinchina@163.com
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Shu-Min
authors.initials: SM
authors.lastname: Zhao
authors.forename: Su-Hua
authors.initials: SH
authors.lastname: Zhang
authors.forename: Cheng-Tao
authors.initials: CT
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Lin
authors.forename: Jun-Hong
authors.initials: JH
authors.lastname: Liu
mesh_terms: D000483:Alleles; D044466:Asian People / Q000235:genetics; D001801:Blood Stains; D002681:China / Q000208:ethnology; D041321:Chromosomes, Human, X / Q000235:genetics*; D016172:DNA Fingerprinting / Q000379:methods; D017931:DNA Primers; D005260:Female; D053803:Forensic Genetics / Q000379:methods; D005787:Gene Frequency; D005819:Genetic Markers; D005828:Genetics, Population; D005838:Genotype; D006197:Hair; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic
chemical_list: D017931:DNA Primers; D005819:Genetic Markers