title: Oxo-ester mediated native chemical ligation on microarrays: an efficient and chemoselective coupling methodology.
abstract: We report a highly efficient and selective method for the coupling of peptides and glycoconjugates bearing N-terminal cysteines to activated surfaces. This chemoselective method generates stable amide linkages without using any thiol additives.
authors.affiliation: School of Chemistry & Manchester Interdisciplinary Biocentre, The University of Manchester, 131 Princess Street, Manchester M17DN, UK.
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Weissenborn
authors.forename: Roberto
authors.initials: R
authors.lastname: Castangia
authors.forename: Johannes W
authors.initials: JW
authors.lastname: Wehner
authors.forename: Robert
authors.initials: R
authors.lastname: Šardzík
authors.forename: Thisbe K
authors.initials: TK
authors.lastname: Lindhorst
authors.forename: Sabine L
authors.initials: SL
authors.lastname: Flitsch
mesh_terms: D003545:Cysteine / Q000737:chemistry; D004952:Esters; D006001:Glycoconjugates / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D040081:Protein Array Analysis / Q000379:methods*; D013379:Substrate Specificity
chemical_list: D004952:Esters; D006001:Glycoconjugates; D010455:Peptides; D003545:Cysteine