title: Expression, purification and crystallization of an indole prenyltransferase from Aspergillus fumigatus.
abstract: CdpNPT from Aspergillus fumigatus is a dimethylallyltryptophan synthase/indole prenyltransferase that catalyzes reverse prenylation at position N1 of tryptophan-containing cyclic dipeptides. Residues 38-440 of CdpNPT were expressed in Escherichia coli and crystallized using the sitting-drop vapour-diffusion and microseeding techniques. The crystals belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 84.4, b = 157.1, c = 161.8 Å, α = β = γ = 90.0°.
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Tokyo 113-0033, Japan.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Morita
authors.forename: Ryohei
authors.initials: R
authors.lastname: Kato
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Noguchi
authors.forename: Shigetoshi
authors.initials: S
authors.lastname: Sugio
authors.forename: Ikuro
authors.initials: I
authors.lastname: Abe
mesh_terms: D001232:Aspergillus fumigatus / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004122:Dimethylallyltranstransferase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D015870:Gene Expression
chemical_list: D004122:Dimethylallyltranstransferase