title: PUFAs in serum cholesterol ester and oxidative DNA damage in Japanese men and women.
abstract: BACKGROUND
  PUFAs are susceptible to lipid peroxidation and play a role in inflammation, both of which can induce oxidative stress. However, the relation of PUFA to oxidative DNA damage in humans is elusive.
  
  
  OBJECTIVE
  We examined the association between concentrations of circulatory PUFAs and urinary 8-oxo-7,8-dihydroguanine (8-oxoGua) in Japanese men and women.
  
  
  METHODS
  The subjects were 495 participants (290 men and 205 women) in a cross-sectional study in 2 municipal offices in Japan. Serum cholesterol ester (CE) and phospholipid fatty acid composition were measured by gas-liquid chromatography. Urinary 8-oxoGua concentrations were measured by HPLC, and 8-oxoGua values for each tertile of PUFA after adjustment for covariates were calculated by multiple regression.
  
  
  RESULTS
  Urinary 8-oxoGua concentrations increased with increasing concentrations of n-3 (omega-3) PUFAs, EPA, and DHA in serum CE (P-trend = 0.001, 0.01, and 0.009, respectively), whereas they decreased with increasing concentrations of n-6 PUFAs and linoleic acid (P-trend = 0.02 and 0.051, respectively).
  
  
  CONCLUSIONS
  Oxidative DNA damage may be greater with higher concentrations of long-chain n-3 PUFAs but lower with higher concentrations of n-6 (omega-6) PUFAs.
authors.affiliation: Department of Nutrition and Life Science, Faculty of Life Science and Biotechnology, Fukuyama University, Fukuyama, Japan. kimura@fubac.fukuyama-u.ac.jp
authors.forename: Yasumi
authors.initials: Y
authors.lastname: Kimura
authors.forename: Masao
authors.initials: M
authors.lastname: Sato
authors.forename: Kayo
authors.initials: K
authors.lastname: Kurotani
authors.forename: Akiko
authors.initials: A
authors.lastname: Nanri
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Kawai
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kasai
authors.forename: Katsumi
authors.initials: K
authors.lastname: Imaizumi
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Mizoue
mesh_terms: D000328:Adult; D000368:Aged; D044466:Asian People; D002784:Cholesterol / Q000097:blood; D002788:Cholesterol Esters / Q000097:blood*; D002849:Chromatography, Gas; D003430:Cross-Sectional Studies; D004249:DNA Damage; D015525:Fatty Acids, Omega-3 / Q000097:blood*; D043371:Fatty Acids, Omega-6 / Q000097:blood*; D005260:Female; D006147:Guanine / Q000031:analogs & derivatives / Q000652:urine; D006801:Humans; D007564:Japan; D016014:Linear Models; D015227:Lipid Peroxidation; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D055815:Young Adult
chemical_list: D002788:Cholesterol Esters; D015525:Fatty Acids, Omega-3; D043371:Fatty Acids, Omega-6; C024829:8-hydroxyguanine; D006147:Guanine; D002784:Cholesterol