title: [Determination of canthaxanthin and astaxanthin in egg yolks by reversed phase high performance liquid chromatography with diode array detection].
abstract: OBJECTIVE
  A method using reversed phase high performance liquid chromatography (RP-HPLC) coupled with diode array detector (DAD) was developed for the simultaneous determination of canthaxanthin and astaxanthin in egg yolks.
  
  
  METHODS
  Samples were extracted with acetonitrile in ultrasonic bath for 20 minutes and then purified by freezing-lipid filtration and solid phase extraction (SPE). After being vaporized to dryness by nitrogen blowing and made up to volume with methanol, the extract solution was chromatographically separated in C18 column with a unitary mobile phase consisting of acetonitrile. The proposed method was validated in terms of linearity, precision, accuracy, and limit of detection (LOD).
  
  
  RESULTS
  Regression analysis revealed a good linearity between peak area of each analyte and its concentration (r > or = 0.998). The intra- and inter-day relative standard deviations (RSDs) were less than 3.6% and 5.2%, respectively. LODs of canthaxanthin and astaxanthin were 0.035 and 0.027 microg/mL (S/N = 3). The average recoveries of canthaxanthin and astaxanthin were 91.5% and 88.7%.
  
  
  CONCLUSIONS
  The proposed method is simple, fast and easy to apply.
authors.affiliation: Department of Sanitary Technology, West China Public Health School, Sichuan University, Chengdu 610041, China.
authors.forename: Kang-Hao
authors.initials: KH
authors.lastname: He
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Zou
authors.forename: Xiang
authors.initials: X
authors.lastname: Liu
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Zeng
mesh_terms: D000818:Animals; D016644:Canthaxanthin / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D056148:Chromatography, Reverse-Phase / Q000379:methods; D004530:Egg Yolk / Q000737:chemistry*; D005505:Food Coloring Agents / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D024341:Xanthophylls / Q000032:analysis
chemical_list: D005505:Food Coloring Agents; D024341:Xanthophylls; D016644:Canthaxanthin; C005948:astaxanthine