title: Biofunctionalization of nanoparticle assisted mass spectrometry as biosensors for rapid detection of plant associated bacteria.
abstract: This study is based on the application of matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) as biosensor to detect the plant associated bacteria (PAB) isolates from rhizospheric soil and root. The rapid bacterial detection via on particle ionization/enrichment technique using IgG functionalized Pt NPs (IgG-Pt NPs) assisted MALDI-TOF MS was successfully used to explore two PAB isolates, namely, Bacillus thuringiensis and B. subtilis from rhizospheric soil and roots of carrot plant. When these bacteria are used as bioformulations in agricultural as well as biotechnological applications, the plant growth promotion of economic crops was observed especially when the crops grow in less fertilize soil regions. This study proved that even at low concentrations, bacteria can also be directly detected without morphological, molecular and biochemical test. The current applied technique is simple, rapid and highly sensitive. Besides, it could be widely used for the detection of beneficially important PAB isolates in environmental samples.
authors.affiliation: Department of Biological Sciences, Faculty of Agriculture, Science and Technology, North-West University, Mafikeng Campus, Private Bag X2046, Mmabatho 2735, South Africa.
authors.forename: Faheem
authors.initials: F
authors.lastname: Ahmad
authors.affiliation: Department of Botany, Aligarh Muslim University, Aligarh 202002, Uttar Pradesh, India.
authors.forename: Mansoor A
authors.initials: MA
authors.lastname: Siddiqui
authors.affiliation: Department of Biological Sciences, Faculty of Agriculture, Science and Technology, North-West University, Mafikeng Campus, Private Bag X2046, Mmabatho 2735, South Africa.
authors.forename: Olubukola O
authors.initials: OO
authors.lastname: Babalola
authors.affiliation: Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, 70, Lien-Hai Road, Kaohsiung, 80424, Taiwan; Center for Nanoscience and Nanotechnology, National Sun Yat-Sen University, 70, Lien-Hai Road, Kaohsiung, 80424, Taiwan; Doctoral Degree Program in Marine Biotechnology, National Sun Yat - Sen University, Kaohsiung, 80424, Taiwan. Electronic address: hwu@faculty.nsysu.edu.
authors.forename: Hui-Fen
authors.initials: HF
authors.lastname: Wu
mesh_terms: D001412:Bacillus subtilis / Q000302:isolation & purification; D001413:Bacillus thuringiensis / Q000302:isolation & purification; D001419:Bacteria / Q000302:isolation & purification*; D015374:Biosensing Techniques / Q000379:methods* / Q000706:statistics & numerical data; D018552:Daucus carota / Q000382:microbiology; D006801:Humans; D007074:Immunoglobulin G; D053768:Metal Nanoparticles / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D018517:Plant Roots / Q000382:microbiology; D010944:Plants / Q000382:microbiology*; D010984:Platinum; D058441:Rhizosphere; D012988:Soil Microbiology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D007074:Immunoglobulin G; D010984:Platinum