title: The effects of sevoflurane and propofol anesthesia on renal sodium-potassium adenosine triphosphatase activity during pneumoperitoneum in rats.
abstract: OBJECTIVE
  To evaluate the renal sodium-potassium adenosine triphosphatase (Na+/K+ATPase) activity, kidney morphology, and the probable protective effects of 2 different anesthetic agents used during pneumoperitoneum (PP).
  
  
  METHODS
  The study was performed at Gazi University Experimental Research Center, Ankara, Turkey between January and July 2009. Twenty-four Wistar albino male rats weighing 320-380 g were randomly allocated to 4 groups after receiving ethics committee approval. All rats were cannulated, intubated, and ventilated under ketamine anesthesia. No further surgical intervention was performed for group I. An intraabdominal pressure (IAP) of 10 mm Hg was created by CO2 insufflation in 18 animals for one hour. The animals in group II received no further anesthetic agents, while the animals in groups III and IV received propofol and sevoflurane. At the end of the protocol, all animals underwent left nephrectomy without sacrificing. Urine was collected from each animal for the following 24 hour for the evaluation of urine creatinine and protein.
  
  
  RESULTS
  The activity of renal Na+/K+ATPase was significantly lower in groups II (p=0.014), III (p=0.019), and IV (p=0.032) compared to group I. The pathological score was significantly higher in groups II (p=0.017), III (p=0.028), and IV (p=0.039) compared to group I.  No statistically significant difference was found among groups II, III, and IV in terms of Na+/K+ATPase activity and pathological scores.
  
  
  CONCLUSIONS
  Elevated IAP is related with impaired kidney functions and morphology, and the so-called renoprotective agents neither improved, nor worsened PP-related renal impairment.
authors.affiliation: Department of Anesthesiology and Reanimation, Faculty of Medicine, Gazi University, Besevler, Ankara, Turkey. kutlukpampal@yahoo.com
authors.forename: Hasan K
authors.initials: HK
authors.lastname: Pampal
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Unal
authors.forename: Cengiz B
authors.initials: CB
authors.lastname: Demirel
authors.forename: Aynur
authors.initials: A
authors.lastname: Albayrak
authors.forename: Omer
authors.initials: O
authors.lastname: Kurtipek
authors.forename: Alper
authors.initials: A
authors.lastname: Murat
authors.forename: Mustafa
authors.initials: M
authors.lastname: Arslan
authors.forename: Mustafa
authors.initials: M
authors.lastname: Kavutcu
mesh_terms: D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D018686:Anesthetics, Intravenous / Q000494:pharmacology*; D000818:Animals; D007668:Kidney / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D008738:Methyl Ethers / Q000494:pharmacology*; D011027:Pneumoperitoneum / Q000201:enzymology*; D015742:Propofol / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D000077149:Sevoflurane; D000254:Sodium-Potassium-Exchanging ATPase / Q000187:drug effects* / Q000378:metabolism
chemical_list: D018685:Anesthetics, Inhalation; D018686:Anesthetics, Intravenous; D008738:Methyl Ethers; D000077149:Sevoflurane; D000254:Sodium-Potassium-Exchanging ATPase; D015742:Propofol