title: Effects of high-mobility group a protein application on canine adipose-derived mesenchymal stem cells in vitro.
abstract: Multipotency and self-renewal are considered as most important features of stem cells to persist throughout life in tissues. In this context, the role of HMGA proteins to influence proliferation of adipose-derived mesenchymal stem cell (ASCs) while maintaining their multipotent and self-renewal capacities has not yet been investigated. Therefore, extracellular HMGA1 and HMGA2 application alone (10-200 ng/mL) and in combination with each other (100, 200 ng/mL each) was investigated with regard to proliferative effects on canine ASCs (cASCs) after 48 hours of cultivation. Furthermore, mRNA expression of multipotency marker genes in unstimulated and HMGA2-stimulated cASCs (50, 100 ng/mL) was analyzed by RT-qPCR. HMGA1 significantly reduced cASCs proliferation in concentrations of 10-200 ng/mL culture medium. A combination of HMGA1 and HMGA2 protein (100 and 200 ng/mL each) caused the same effects, whereas no significant effect on cASCs proliferation was shown after HMGA2 protein application alone. RT-qPCR results showed that expression levels of marker genes including KLF4, SOX2, OCT4, HMGA2, and cMYC mRNAs were on the same level in both HMGA2-protein-stimulated and -unstimulated cASCs. Extracellular HMGA protein application might be valuable to control proliferation of cASCs in context with their employment in regenerative approaches without affecting their self-renewal and multipotency abilities.
authors.affiliation: Small Animal Hospital, University of Veterinary Medicine Foundation, 30559 Hannover, Germany.
authors.forename: A A
authors.initials: AA
authors.lastname: Ismail
authors.forename: S
authors.initials: S
authors.lastname: Wagner
authors.forename: H
authors.initials: H
authors.lastname: Murua Escobar
authors.forename: S
authors.initials: S
authors.lastname: Willenbrock
authors.forename: K A
authors.initials: KA
authors.lastname: Sterenczak
authors.forename: M T
authors.initials: MT
authors.lastname: Samy
authors.forename: A M
authors.initials: AM
authors.lastname: Abd El-Aal
authors.forename: I
authors.initials: I
authors.lastname: Nolte
authors.forename: P
authors.initials: P
authors.lastname: Wefstaedt