title: Effects of different calcium, magnesium, and zinc concentrations supplemented on hepatopancreatic cell proliferation of kuruma prawn, Penaeus japonicus.
abstract: The effects of different calcium (Ca(2+)), magnesium (Mg(2+)), and zinc (Zn(2+)) concentrations supplemented on hepatopancreatic cell proliferation of kuruma prawn, Penaeus japonicus was studied. The culture system consists of medium 199 (M 199) supplemented with 0.060 mol/L NaCl, 1.011 g/L glucose, 1,000 UI/ml penicillin, 1,000 μg/ml treptomycin, heat inactivated fetal calf serum (FCS) 20% for primary cell culture and 10% for subculture. The RNA/DNA ratio of the cell cultures was measured. The results show that the cell division of hepatopancreatic cells of P. japonicus was enhanced by the optimal concentration of inorganic salt (Ca(2+), 1.0 g/L; Mg(2+), 5.0 g/L; Zn(2+), 80 μg/L). The hepatopancreatic cell culture system and improved culture conditions described here will be very useful for in vitro experiments to study viruses responsible for infections in shrimp leading to tremendous economic losses.
authors.affiliation: South China Institute of Environmental Sciences, Ministry of Environmental Protection, Guangzhou, People's Republic of China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Wang
authors.forename: Duanbo
authors.initials: D
authors.lastname: Cai
authors.forename: Kaiming
authors.initials: K
authors.lastname: Li
authors.forename: Haiming
authors.initials: H
authors.lastname: Xu
authors.forename: Likun
authors.initials: L
authors.lastname: Yang
authors.forename: Na
authors.initials: N
authors.lastname: Zhu
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002448:Cell Adhesion; D002455:Cell Division / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D003470:Culture Media / Q000737:chemistry; D004247:DNA / Q000032:analysis; D005947:Glucose / Q000737:chemistry; D043143:Hepatopancreas / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology*; D033561:Penaeidae / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010406:Penicillins / Q000737:chemistry; D061251:Primary Cell Culture; D012313:RNA / Q000032:analysis; D044967:Serum / Q000737:chemistry; D015032:Zinc / Q000494:pharmacology*
chemical_list: D003470:Culture Media; D010406:Penicillins; D012313:RNA; D004247:DNA; D008274:Magnesium; D005947:Glucose; D015032:Zinc; D002118:Calcium