title: Neuronal cell cycle re-entry markers are altered in the senescence accelerated mouse P8 (SAMP8).
abstract: Senescence-accelerated mice 8 (SAMP8), a model of aging, display many established pathological features of Alzheimer's disease (AD); however, whether cell cycle alterations exist in these animals remains unknown. Given that these animals present changes such as tau phosphorylation and redox imbalance, both associated with cell cycle alterations, we determined whether changes in cell cycle markers were present in SAMP8 and SAMR1 (control strain) at 3, 6, and 9 months-old brains. As expected, an increase in tau hyperphosphorylation and its associated machinery, i.e., cdk5 and GSK3β, was observed both between strains and also with aging. Particularly, significant differences in cyclin A, cyclin D1, cyclin E, Cdk2, cyclin B, pR, and E2F1 were found when comparing SAMP8 to SAMR1. More interestingly, a partial correlation with several cell cycle markers described in AD brain is found in SAMP8, indicating that some specific hallmarks of AD are also present in this strain, which has been postulated as an early switch model of the disease.
authors.affiliation: Departments of Neurosciences, Case Western Reserve University School of Medicine, Cleveland, OH, USA.
authors.forename: Gemma
authors.initials: G
authors.lastname: Casadesús
authors.forename: Javier
authors.initials: J
authors.lastname: Gutierrez-Cuesta
authors.forename: Hyoung-Gon
authors.initials: HG
authors.lastname: Lee
authors.forename: Andrés
authors.initials: A
authors.lastname: Jiménez
authors.forename: Marta
authors.initials: M
authors.lastname: Tajes
authors.forename: Daniel
authors.initials: D
authors.lastname: Ortuño-Sahagún
authors.forename: Antoni
authors.initials: A
authors.lastname: Camins
authors.forename: Mark A
authors.initials: MA
authors.lastname: Smith
authors.forename: Mercè
authors.initials: M
authors.lastname: Pallàs
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002453:Cell Cycle / Q000502:physiology*; D059447:Cell Cycle Checkpoints / Q000502:physiology*; D051360:Cyclin-Dependent Kinase 5 / Q000378:metabolism; D038362:Glycogen Synthase Kinase 3 / Q000378:metabolism; D000071679:Glycogen Synthase Kinase 3 beta; D008297:Male; D051379:Mice; D023421:Models, Animal; D009474:Neurons / Q000378:metabolism*; D010766:Phosphorylation; D016875:tau Proteins / Q000378:metabolism
chemical_list: D015415:Biomarkers; D016875:tau Proteins; D051360:Cyclin-Dependent Kinase 5; D000071679:Glycogen Synthase Kinase 3 beta; C000605507:Gsk3b protein, mouse; D038362:Glycogen Synthase Kinase 3