title: PCV2-DNA in formalin-fixed and paraffin embedded lymph nodes of wild boar (Sus scrofa ssp. scrofa): one sampling approach for two laboratory techniques.
abstract: Superficial inguinal lymph nodes from 72 wild boars examined in a previous immunohistochemical (IHC) study on porcine circovirus type 2 (PCV2) were selected for a PCV2 polymerase chain reaction (PCR) analysis. Four of these lymph nodes were PCV2-IHC strongly positive with PMWS histological lesions (outcome 1), 6 weak to mild PCV2-IHC positive without PMWS histological lesions (outcome 2) and 62 PCV2-IHC negative. Considering IHC the gold standard for diagnosis, the aims of the study were to evaluate the suitability of the PCV2-DNA extraction from formalin-fixed and paraffin-embedded (FFPE) tissue and the sensitivity and specificity of PCR under two IHC interpretations criteria: (A) the sample was considered positive if the result was outcome 1; (B) the sample was considered positive if the result was outcome 1 or 2. Under (A) criteria, sensitivity and specificity of PCR were 100% and 89.7%, respectively; the Cohen's Kappa coefficient was 0.49. Under (B) criteria, sensitivity and specificity of PCR were 80.0% and 95.2%, respectively; the Cohen's Kappa coefficient was 0.72. The high Cohen's Kappa coefficient under the (B) interpretative criteria indicates good agreement between the two methods. In conclusion, 1) DNA extracted from FFPE specimens of wild boar is suitable for PCR and further represents a screening test for PCV2/PCVD (PCV2 Diseases) investigations in wild boar as well; 2) routine histological sampling can also be useful for PCV2 virological studies in wild boar.
authors.affiliation: Department of Veterinary Medical Science, University of Bologna, via Tolara di Sopra 50, 40064 Ozzano dell'Emilia, Bologna, Italy. federico.morandi@unibo.it
authors.forename: Federico
authors.initials: F
authors.lastname: Morandi
authors.forename: Serena
authors.initials: S
authors.lastname: Panarese
authors.forename: Ranieri
authors.initials: R
authors.lastname: Verin
authors.forename: Fabio
authors.initials: F
authors.lastname: Ostanello
authors.forename: Cinzia
authors.initials: C
authors.lastname: Benazzi
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Sarli
mesh_terms: D000818:Animals; D018173:Circoviridae Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000302:isolation & purification*; D004279:DNA, Viral / Q000032:analysis; D005557:Formaldehyde / Q000737:chemistry; D007150:Immunohistochemistry / Q000379:methods / Q000662:veterinary; D008198:Lymph Nodes / Q000473:pathology / Q000821:virology; D016612:Paraffin Embedding / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000662:veterinary; D053570:Porcine Postweaning Multisystemic Wasting Syndrome / Q000175:diagnosis* / Q000821:virology; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000662:veterinary; D013552:Swine
chemical_list: D004279:DNA, Viral; D005557:Formaldehyde