title: Differential cellular and microbial responses to nano-/micron-scale titanium surface roughness induced by hydrogen peroxide treatment.
abstract: This study investigated the effects of combined titanium nano-/micron-scale roughness, induced by hydrogen peroxide pre-treatments, on bone marrow stromal cell responses and Porphyromonas gingivalis adherence in vitro. Untreated surfaces exhibited nano-scale features, while hydrogen peroxide treatments promoted increased nano-/micron-scale roughness. Bone marrow stromal cell attachment and proliferation were maintained with 6 h and 24 h treatments, but significantly decreased on 1-week and 4-week-treated surfaces. Bone marrow stromal cells on 6 h-4 week-treated titanium demonstrated enhanced osteogenic differentiation versus untreated surfaces. P. gingivalis adherence was significantly increased on 24 h-4 week surfaces. Results suggest that 6 h but less than 24 h treatments maintain or promote bone marrow stromal cell responses while minimizing microbial adherence, potentially enhancing titanium surface bio-activation for osseointegration.
authors.affiliation: Tissue Engineering & Reparative Dentistry, Cardiff Institute of Tissue Engineering & Repair-CITER, School of Dentistry, Cardiff University, Cardiff, UK.
authors.forename: Abdulaziz E
authors.initials: AE
authors.lastname: Daw
authors.forename: Hizbullah Aa
authors.initials: HA
authors.lastname: Kazi
authors.forename: John S
authors.initials: JS
authors.lastname: Colombo
authors.forename: Wendy G
authors.initials: WG
authors.lastname: Rowe
authors.forename: David W
authors.initials: DW
authors.lastname: Williams
authors.forename: Rachel J
authors.initials: RJ
authors.lastname: Waddington
authors.forename: David W
authors.initials: DW
authors.lastname: Thomas
authors.forename: Ryan
authors.initials: R
authors.lastname: Moseley
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible; D015921:Dental Implants; D006861:Hydrogen Peroxide; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology; D049329:Nanostructures; D016348:Osseointegration; D016966:Porphyromonas gingivalis / Q000502:physiology; D051381:Rats; D013499:Surface Properties; D014025:Titanium
chemical_list: D020099:Coated Materials, Biocompatible; D015921:Dental Implants; D006861:Hydrogen Peroxide; D014025:Titanium