title: Effect of magnolol on the function of osteoblastic MC3T3-E1 cells.
abstract: OBJECTIVE
  In the present study, the ability of magnolol, a hydroxylated biphenyl compound isolated from Magnolia officinalis, to stimulate osteoblast function and inhibit the release of bone-resorbing mediators was investigated in osteoblastic MC3T3-E1 cells.
  
  
  METHODS
  Osteoblast function was measured by cell growth, alkaline phosphatase activity, collagen synthesis, and mineralization. Glutathione content was also measured in the cells. Bone-resorbing cytokines, receptor activator of nuclear factor-κB ligand (RANKL), TNF-α, and IL-6 were measured with an enzyme immunoassay system.
  
  
  RESULTS
  Magnolol caused a significant elevation of cell growth, alkaline phosphatase activity, collagen synthesis, mineralization, and glutathione content in the cells (P < 0.05). Skeletal turnover is orchestrated by a complex network of regulatory factors. Among cytokines, RANKL, TNF-α, and IL-6 were found to be key osteoclastogenetic molecules produced by osteoblasts. Magnolol significantly (P < 0.05) decreased the production of osteoclast differentiation inducing factors such as RANKL, TNF-α, and IL-6 in the presence of antimycin A, which inhibits mitochondrial electron transport and has been used as an ROS generator.
  
  
  CONCLUSIONS
  Magnolol might be a candidate as an agent for the prevention of bone disorders such as osteoporosis.
authors.affiliation: Department of Food Science Technology, Yeungnam University, Gyeongsan 712-749, Republic of Korea.
authors.forename: Eun Jung
authors.initials: EJ
authors.lastname: Kwak
authors.forename: Young Soon
authors.initials: YS
authors.lastname: Lee
authors.forename: Eun Mi
authors.initials: EM
authors.lastname: Choi
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000968:Antimycin A / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000494:pharmacology*; D002460:Cell Line; D003094:Collagen / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D017705:Lignans / Q000494:pharmacology*; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism*; D053245:RANK Ligand / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D001713:Biphenyl Compounds; D016207:Cytokines; D015850:Interleukin-6; D017705:Lignans; D053245:RANK Ligand; D014409:Tumor Necrosis Factor-alpha; C005498:magnolol; D000968:Antimycin A; D003094:Collagen; D000469:Alkaline Phosphatase; D005978:Glutathione