title: Detection of adiponectin and monocyte chemoattractant protein-1 using a calixcrown derivatives-coated protein chip.
abstract: We used a ProteoChip coated with a calixcrown derivative protein linker to measure adiponectin and monocyte chemoattractant protein-1 (MCP-1) levels and compared the results with commercial enzyme-linked immunosorbent assay (ELISA) kits. Adiponectin and MCP-1 levels in normal human serum and RAW264 cell supernatants, respectively, were measured. The ProteoChip quantification results correlated with those from the ELISA kits; however, the ProteoChip required less sample volume, exhibited higher sensitivity, and had a wider detection range. The ProteoChip was capable of detecting and quantifying small amounts of protein, possibly replacing ELISA kits in evaluating the levels of adiponectin and MCP-1.
authors.affiliation: Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
authors.forename: Rima
authors.initials: R
authors.lastname: Tanabe
authors.forename: Byung-Yoon
authors.initials: BY
authors.lastname: Cha
authors.forename: Byung Geun
authors.initials: BG
authors.lastname: Ha
authors.forename: Takayuki
authors.initials: T
authors.lastname: Yonezawa
authors.forename: Kazuo
authors.initials: K
authors.lastname: Nagai
authors.forename: Je-Tae
authors.initials: JT
authors.lastname: Woo
authors.forename: Kazumi
authors.initials: K
authors.lastname: Yagasaki
mesh_terms: D052242:Adiponectin / Q000032:analysis* / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D047250:Calixarenes / Q000737:chemistry* / Q000276:immunology; D002460:Cell Line; D018932:Chemokine CCL2 / Q000032:analysis* / Q000276:immunology; D043844:Crown Ethers / Q000737:chemistry* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D051379:Mice; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods*; D011994:Recombinant Proteins / Q000032:analysis / Q000276:immunology; D012680:Sensitivity and Specificity
chemical_list: D052242:Adiponectin; D000911:Antibodies, Monoclonal; C111835:CCL2 protein, human; D018932:Chemokine CCL2; D043844:Crown Ethers; D011994:Recombinant Proteins; D047250:Calixarenes