title: A relation between TGF-β and mast cell tryptase in experimental emphysema models.
abstract: Chronic obstructive pulmonary disease (COPD) is a multicomponent disease characterized by emphysema and/or chronic bronchitis. The aim of this study was to investigate the effect of cigarette smoke exposure on mast cells and mast cell function in vitro and in vivo in order to get further insight in the role of mast cells in the pathogenesis of emphysema. Cigarette smoke conditioned medium (CSM) induced the expression of mast cell tryptase (MMCP-6) in primary cultured mast cells. This tryptase expression was caused by the CSM-stimulated production of TGF-β in culture and neutralization of TGF-β suppressed the CSM-induced expression of tryptase in mast cells. An increase in mast cell tryptase expression was also found in an experimental model for emphysema. Exposure of mice to cigarette smoke increased the number of mast cells in the airways and the expression of mast cell tryptase. In accordance with the in vitro findings, TGF-β in bronchoalveolar lavage fluid of smoke-exposed animals was significantly increased. Our study indicates that mast cells may be a source of TGF-β production after cigarette smoke exposure and that in turn TGF-β may change the tryptase expression in mast cells.
authors.affiliation: Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, The Netherlands. emortaz@gmail.com
authors.forename: Esmaeil
authors.initials: E
authors.lastname: Mortaz
authors.forename: Masoumeh E
authors.initials: ME
authors.lastname: Givi
authors.forename: Carla A
authors.initials: CA
authors.lastname: Da Silva
authors.forename: Gert
authors.initials: G
authors.lastname: Folkerts
authors.forename: Frank A
authors.initials: FA
authors.lastname: Redegeld
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D008407:Mast Cells / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D029424:Pulmonary Disease, Chronic Obstructive / Q000139:chemically induced / Q000201:enzymology* / Q000473:pathology; D011656:Pulmonary Emphysema / Q000139:chemically induced / Q000201:enzymology* / Q000473:pathology; D012906:Smoke / Q000009:adverse effects*; D012907:Smoking / Q000009:adverse effects*; D014026:Nicotiana / Q000009:adverse effects; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D053802:Tryptases / Q000378:metabolism*
chemical_list: D017077:Culture Media, Conditioned; D012906:Smoke; D016212:Transforming Growth Factor beta; D053802:Tryptases