title: Preparation, structure and anticoagulant activity of a low molecular weight fraction produced by mild acid hydrolysis of sulfated rhamnan from Monostroma latissimum.
abstract: A low molecular weight fraction, designated LMWP, was prepared by mild acid hydrolysis of sulfated rhamnan from Monostroma latissimum and purified by anion-exchange and gel-permeation chromatography. Chemical and spectroscopic analyses showed that LMWP was mainly composed of rhamnose, and its molecular weight was about 33.6 kDa. The backbone of LMWP consists of 1,3-linked α-L-rhamnose units with partially sulfate groups at the C-2 position. Approximately 25% of 1,3-linked α-L-rhamnose units is substituted at C-2 by sulfated or non-sulfated 1,3-linked α-L-rhamnose and 1,2-linked α-L-rhamnose units. LMWP effectively prolonged clotting time as evaluated by the activated partial thromboplastin time assay and was a potent thrombin inhibitor mediated by heparin cofactor II. The investigation demonstrated that LMWP is a novel sulfated polysaccharide with anticoagulant activity.
authors.affiliation: Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, 5 Yushan Road, Qingdao 266003, People's Republic of China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Li
authors.forename: Wenjun
authors.initials: W
authors.lastname: Mao
authors.forename: Yujiao
authors.initials: Y
authors.lastname: Hou
authors.forename: Yan
authors.initials: Y
authors.lastname: Gao
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Qi
authors.forename: Chunqi
authors.initials: C
authors.lastname: Zhao
authors.forename: Yin
authors.initials: Y
authors.lastname: Chen
authors.forename: Yanli
authors.initials: Y
authors.lastname: Chen
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.forename: Chunyan
authors.initials: C
authors.lastname: Wang
mesh_terms: D000143:Acids / Q000737:chemistry; D000925:Anticoagulants / Q000138:chemical synthesis / Q000494:pharmacology; D001777:Blood Coagulation / Q000187:drug effects*; D000460:Chlorophyta / Q000737:chemistry*; D003837:Deoxy Sugars / Q000138:chemical synthesis* / Q000494:pharmacology*; D006801:Humans; D006868:Hydrolysis; D008351:Mannans / Q000138:chemical synthesis* / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D013431:Sulfates / Q000737:chemistry
chemical_list: D000143:Acids; D000925:Anticoagulants; D003837:Deoxy Sugars; D008351:Mannans; D010936:Plant Extracts; D013431:Sulfates; C065836:rhamnan