title: Preparative 2D Gel Electrophoresis with Immobilized pH Gradients: Rehydration of IPG Strips for Isoelectric Focusing of Proteins.
abstract: INTRODUCTIONThis protocol describes a method for rehydration of IPG gel strips in preparation for their use for isoelectric focusing (IEF) on immobilized pH gradient (IPG) gels. Following rehydration, IEF can be performed using either a flatbed unit or a self-contained instrument.
authors.forename: Wayne R
authors.initials: WR
authors.lastname: Stochaj
authors.forename: Tom
authors.initials: T
authors.lastname: Berkelman
authors.forename: Nancy
authors.initials: N
authors.lastname: Laird