title: Oligopeptides functionalized surface plasmon resonance biosensors for detecting thiacloprid and imidacloprid.
abstract: By using phage display library, we identified two highly specific oligopeptide sequences RKRIRRMMPRPS and RNRHTHLRTRPR for binding neonicotinoids such as thiacloprid and imidacloprid. The former shows high affinity for thiacloprid whereas the latter shows high affinity for imidacloprid. Surprisingly, cross binding is minimal despite the similarity of the two molecules. To develop a neonicotinoid biosensor, these two oligopeptides are synthesized and immobilized on the surface of a surface plasmon resonance (SPR) chip with a bare-gold surface. This oligopeptide functionalized SPR biosensor can rapidly detect thiacloprid and imidacloprid in buffer solutions in a real-time manner. The limit of detection (LOD) for thiacloprid and imidacloprid is 1.2 μM and 0.9 μM, respectively.
authors.affiliation: Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117576, Singapore.
authors.forename: Xiaokang
authors.initials: X
authors.lastname: Ding
authors.affiliation: Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117576, Singapore.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Civil and Environmental Engineering, National University of Singapore, 1 Engineering Drive 3, Singapore 117576, Singapore.
authors.forename: Dan
authors.initials: D
authors.lastname: Cheng
authors.affiliation: Department of Civil and Environmental Engineering, National University of Singapore, 1 Engineering Drive 3, Singapore 117576, Singapore.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: He
authors.affiliation: Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117576, Singapore. Electronic address: cheyk@nus.edu.sg.
authors.forename: Kun-Lin
authors.initials: KL
authors.lastname: Yang
mesh_terms: D000595:Amino Acid Sequence; D007093:Imidazoles / Q000032:analysis*; D055767:Immobilized Proteins / Q000737:chemistry; D057230:Limit of Detection; D000073943:Neonicotinoids; D009538:Nicotine / Q000031:analogs & derivatives / Q000032:analysis; D009574:Nitro Compounds / Q000032:analysis*; D009842:Oligopeptides / Q000737:chemistry; D019151:Peptide Library; D011485:Protein Binding; D011725:Pyridines / Q000032:analysis*; D011978:Receptors, Nicotinic / Q000378:metabolism; D020349:Surface Plasmon Resonance / Q000379:methods* / Q000706:statistics & numerical data; D013843:Thiazines / Q000032:analysis*
chemical_list: D007093:Imidazoles; D055767:Immobilized Proteins; D000073943:Neonicotinoids; D009574:Nitro Compounds; D009842:Oligopeptides; D019151:Peptide Library; D011725:Pyridines; D011978:Receptors, Nicotinic; D013843:Thiazines; C082359:imidacloprid; D009538:Nicotine; C417209:thiacloprid