title: Natural compounds inhibiting the replication of Porcine reproductive and respiratory syndrome virus.
abstract: Porcine reproductive and respiratory syndrome virus (PRRSV) is an economically important pathogenic virus in the swine production. Current vaccines against PRRSV do not induce sterile immunity and the virus evolves at a rapid rate with frequent appearances of new strains. In this study, we screened a library of 502 highly purified natural product compounds to identify specific inhibitors of PRRSV replication cycle. Our observations showed that many of the inhibitory compounds identified have activity on the cellular ion transport mechanisms. We identified for the first time, four compounds which inhibit the PRRSV replication cycle at micro molar concentration or less, namely, 12-deoxyphorbol 13-phenylacetate 20-acetate, ouabain, bufalin and valinomycin. Further, we have identified 15 other compounds which can inhibit the PRRSV replication at the concentration of 8μM. This study provides a basis for further development of pharmacological agents to inhibit PRRSV replication.
authors.affiliation: Animal Health Biotechnology Group, Temasek Lifesciences Laboratory, National University of Singapore, Singapore, Singapore.
authors.forename: Anbu K
authors.initials: AK
authors.lastname: Karuppannan
authors.forename: Kan Xing
authors.initials: KX
authors.lastname: Wu
authors.forename: Jia
authors.initials: J
authors.lastname: Qiang
authors.forename: Justin Jang-Hann
authors.initials: JJ
authors.lastname: Chu
authors.forename: Jimmy
authors.initials: J
authors.lastname: Kwang
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001688:Biological Products / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D000882:Haplorhini; D019316:Porcine respiratory and reproductive syndrome virus / Q000187:drug effects* / Q000502:physiology; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D000998:Antiviral Agents; D001688:Biological Products