title: Protective effect of Panax ginseng against serum biochemical changes and apoptosis in kidney of rats treated with gentamicin sulphate.
abstract: The protective effects of Panax ginseng (PG) on gentamicin sulphate (GS) induced acute nephrotoxicity were investigated in rats. A total of 32 adult Sprague-Dawley rats were randomly divided into 4 equal groups and treated by intraperitoneous route for 10 days with: 0.5 mL of isotonic saline (group C), GS 100 mg/kg/day (group GS), co treatment PG (100 and 200 mg/kg/day) plus GS (100 mg/kg/day). After the last injection, kidney markers (urea, creatinine and blood urea nitrogen-BUN) and hepatic markers (aspartate aminotransferase-AST, alanine aminotransferase-ALT, gama glutamil transferase-GGT), and biochemical parameters were analyzed using diagnostic kits. Also, kidney changes were evaluated by immunohistochemical and stereological methods. GS treatment induced significant elevation (P < 0.05) in kidney and hepatic markers, most of biochemical parameters, and Bax immunoreactivity as well. However, co treatments with both doses of PG (100 and 200 mg/kg/day) significantly alleviated (P < 0.05) the GS-induced elevations and have partially protected rats from nephrotoxicity (reduction of kidney damage, and of urea, creatinine and BUN concentrations, and of apoptotic index). Both biochemical results and immunohistochemical evidence showed that administration of PG reduced the gentamicin-induced nephrotoxicity.
authors.affiliation: Department of Histology and Embryology, University of Rize, Rize, Turkey.
authors.forename: Yildiray
authors.initials: Y
authors.lastname: Kalkan
authors.forename: Kubra Asena Terim
authors.initials: KA
authors.lastname: Kapakin
authors.forename: Adem
authors.initials: A
authors.lastname: Kara
authors.forename: Tennur
authors.initials: T
authors.lastname: Atabay
authors.forename: Ali
authors.initials: A
authors.lastname: Karadeniz
authors.forename: Nejdet
authors.initials: N
authors.lastname: Simsek
authors.forename: Emre
authors.initials: E
authors.lastname: Karakus
authors.forename: Ismail
authors.initials: I
authors.lastname: Can
authors.forename: Serap
authors.initials: S
authors.lastname: Yildirim
authors.forename: Seckin
authors.initials: S
authors.lastname: Ozkanlar
authors.forename: Emin
authors.initials: E
authors.lastname: Sengul
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001806:Blood Urea Nitrogen; D003404:Creatinine / Q000097:blood; D005839:Gentamicins / Q000633:toxicity*; D007150:Immunohistochemistry; D007668:Kidney / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D017093:Liver Failure / Q000139:chemically induced / Q000188:drug therapy / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects; D005894:Panax / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D020011:Protective Agents / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D051437:Renal Insufficiency / Q000139:chemically induced / Q000188:drug therapy / Q000473:pathology
chemical_list: D005839:Gentamicins; D010936:Plant Extracts; D020011:Protective Agents; D003404:Creatinine