title: Macroporous methacrylate-based monoliths as platforms for DNA microarrays.
abstract: Macroporous monoliths with different surface functionalization (reactive groups) were utilized as platforms for DNA analysis in microarray format. The slides based on a copolymer glycidyl methacrylate-co-ethylene dimethacrylate (GMA-EDMA) have been chosen as well known and thoroughly studied standard. In particular, this material has been used at optimization of DNA microanalytical procedure. The concentration and pH of spotting solution, immobilization temperature and time, blocking agent and coupling reaction duration were selected as varied parameters. The efficiency of analysis performed on 3-D monolithic platforms was compared to that established for commercially available glass slides. As a practical example, a diagnostic test for detection of CFTR gene mutation was carried out. Additionally, the part of presented work was devoted to preparation of aptamer-based test-system that allowed successful and highly sensitive detection both of DNA and protein.
authors.affiliation: Institute of Macromolecular Compounds, Russian Academy of Sciences, Bolshoy pr. 31, 199004 St. Petersburg, Russia.
authors.forename: Ekaterina S
authors.initials: ES
authors.lastname: Sinitsyna
authors.forename: Johanna G
authors.initials: JG
authors.lastname: Walter
authors.forename: Evgenia G
authors.initials: EG
authors.lastname: Vlakh
authors.forename: Frank
authors.initials: F
authors.lastname: Stahl
authors.forename: Cornelia
authors.initials: C
authors.lastname: Kasper
authors.forename: Tatiana B
authors.initials: TB
authors.lastname: Tennikova
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000378:metabolism; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics / Q000378:metabolism; D005898:Glass / Q000737:chemistry; D008689:Methacrylates / Q000737:chemistry*; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015345:Oligonucleotide Probes / Q000737:chemistry / Q000235:genetics; D011108:Polymers / Q000737:chemistry; D016062:Porosity; D011327:Printing; D013499:Surface Properties
chemical_list: D052157:Aptamers, Nucleotide; D008689:Methacrylates; D015345:Oligonucleotide Probes; D011108:Polymers; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; C008384:methacrylic acid