title: Intracerebroventricular administration of neuronostatin delays gastric emptying and gastrointestinal transit in mice.
abstract: Neuronostatin is a 13-amino acid amidated peptide widely distributed in various organs including gastrointestinal tract. However, the effect of neuronostatin on gastrointestinal motility has not been well characterized. In the present work, effects of central administration of neuronostatin on gastric emptying and gastrointestinal transit were investigated. The results indicated that intracerebroventricular (i.c.v.) administration of neuronostatin (1, 5, 10 or 20nmol/mouse) delayed gastric emptying and gastrointestinal transit in a dose-related manner in mice. The effects were significantly reversed by melanocortin 3/4 receptor antagonist SHU9119 or classical opioid receptor antagonist naloxone, suggesting that the central melanocortin system and opioid system may be involved in the gastrointestinal effects elicited by i.c.v. administration of neuronostatin. In addition, we found that C-terminal amidation modification of neuronostatin is essential to exert its gastrointestinal effects. These results indicated that neuronostatin may play an important role in regulating gastrointestinal function.
authors.affiliation: Institute of Biochemistry and Molecular Biology, School of Life Sciences, Lanzhou University, 222 Tian Shui South Road, Lanzhou 730000, PR China.
authors.forename: Shu-Fang
authors.initials: SF
authors.lastname: Su
authors.forename: Ai-Min
authors.initials: AM
authors.lastname: Yang
authors.forename: Shao-Bin
authors.initials: SB
authors.lastname: Yang
authors.forename: Ning-Bo
authors.initials: NB
authors.lastname: Wang
authors.forename: Song-Song
authors.initials: SS
authors.lastname: Lu
authors.forename: Hui-Hui
authors.initials: HH
authors.lastname: Wang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Chen
mesh_terms: D000818:Animals; D002552:Cerebral Ventricles / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D005746:Gastric Emptying / Q000187:drug effects; D005772:Gastrointestinal Transit / Q000187:drug effects; D008297:Male; D009074:Melanocyte-Stimulating Hormones / Q000494:pharmacology; D051379:Mice; D009270:Naloxone / Q000494:pharmacology; D009292:Narcotic Antagonists / Q000494:pharmacology; D036361:Peptide Hormones / Q000008:administration & dosage / Q000502:physiology*; D044101:Receptors, Melanocortin / Q000819:agonists
chemical_list: D009292:Narcotic Antagonists; D036361:Peptide Hormones; D044101:Receptors, Melanocortin; C543371:neuronostatin, mouse; C103272:SHU 9119; D009270:Naloxone; D009074:Melanocyte-Stimulating Hormones