title: Regulation of miRNA 219 and miRNA Clusters 338 and 17-92 in Oligodendrocytes.
abstract: MicroRNAs (miRs) regulate diverse molecular and cellular processes including oligodendrocyte (OL) precursor cell (OPC) proliferation and differentiation in rodents. However, the role of miRs in human OPCs is poorly understood. To identify miRs that may regulate these processes in humans, we isolated OL lineage cells from human white matter and analyzed their miR profile. Using endpoint RT-PCR assays and quantitative real-time PCR, we demonstrate that miR-219, miR-338, and miR-17-92 are enriched in human white matter and expressed in acutely isolated human OLs. In addition, we report the expression of closely related miRs (miR-219-1-3p, miR-219-2-3p, miR-1250, miR-657, miR-3065-5p, miR-3065-3p) in both rodent and human OLs. Our findings demonstrate that miRs implicated in rodent OPC proliferation and differentiation are regulated in human OLs and may regulate myelination program in humans. Thus, these miRs should be recognized as potential therapeutic targets in demyelinating disorders.
authors.affiliation: Department of Neurology and Neurosurgery, Montreal Neurological Institute and Hospital, McGill University Health Centre, McGill University Montreal, QC, Canada.
authors.forename: Omar
authors.initials: O
authors.lastname: de Faria
authors.forename: Qiao-Ling
authors.initials: QL
authors.lastname: Cui
authors.forename: Jenea M
authors.initials: JM
authors.lastname: Bin
authors.forename: Sarah-Jane
authors.initials: SJ
authors.lastname: Bull
authors.forename: Timothy E
authors.initials: TE
authors.lastname: Kennedy
authors.forename: Amit
authors.initials: A
authors.lastname: Bar-Or
authors.forename: Jack P
authors.initials: JP
authors.lastname: Antel
authors.forename: David R
authors.initials: DR
authors.lastname: Colman
authors.forename: Ajit S
authors.initials: AS
authors.lastname: Dhaunchak