title: Simultaneous electrochemical determination of uric acid, xanthine and hypoxanthine based on poly(L-arginine)/graphene composite film modified electrode.
abstract: Poly(l-arginine)/graphene composite film modified electrode was successfully prepared via a facile one-step electrochemical method and used for simultaneous determination of uric acid (UA), xanthine (XA) and hypoxanthine (HX). The electrochemical behaviors of UA, XA and HX at the modified electrode were studied by cyclic voltammetry and differential pulse voltammetry (DPV), and showed that the modified electrode exhibited excellent electrocatalytic activity toward the oxidation of the three compounds. The calibration curves for UA, XA and HX were obtained over the range of 0.10-10.0, 0.10-10.0 and 0.20-20.0 μM by DPV, respectively and the detection limits for UA, XA and HX were 0.05, 0.05 and 0.10 μM (S/N=3), respectively. With good selectivity and high sensitivity, the modified electrode has been applied to simultaneous determination of UA, XA and HX in human urine with satisfactory result.
authors.affiliation: Department of Chemistry, Lanzhou University, Lanzhou 730000, PR China.
authors.forename: Fengyuan
authors.initials: F
authors.lastname: Zhang
authors.forename: Zaihua
authors.initials: Z
authors.lastname: Wang
authors.forename: Yuzhen
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zhixiang
authors.initials: Z
authors.lastname: Zheng
authors.forename: Chunming
authors.initials: C
authors.lastname: Wang
authors.forename: Yongling
authors.initials: Y
authors.lastname: Du
authors.forename: Weichun
authors.initials: W
authors.lastname: Ye
mesh_terms: D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D019271:Hypoxanthine / Q000032:analysis* / Q000737:chemistry / Q000652:urine; D016014:Linear Models; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*; D015203:Reproducibility of Results; D013997:Time Factors; D014527:Uric Acid / Q000032:analysis* / Q000737:chemistry / Q000652:urine; D019820:Xanthine / Q000032:analysis* / Q000737:chemistry / Q000652:urine
chemical_list: D010087:Oxides; D010455:Peptides; D019820:Xanthine; C015462:polyarginine; D014527:Uric Acid; D019271:Hypoxanthine; D006108:Graphite