title: Development of electrochemiluminescence-based singleplex and multiplex assays for the quantification of α-synuclein and other proteins in cerebrospinal fluid.
abstract: The need for improved diagnostic accuracy and markers of progression in neurodegenerative diseases motivates the identification of objective biomarkers as well as optimized assays for their quantification. Several potential marker candidates for Parkinson's disease (PD) in cerebrospinal fluid have been identified. These include α-synuclein, a major constituent of the intracellular aggregates. We give a general overview and details of our experience in converting established enzyme-linked immunoabsorbent assays (for α-synuclein and other proteins) onto an electrochemiluminescence-based platform as well as considerations on multiplexing different assays for PD.
authors.affiliation: Institute for Neuropathology, Prion and Dementia Research Unit, University Medical Center Göttingen, Robert-Koch-Str. 40, 37075 Goettingen, Germany. n.kruse@med.uni-goettingen.de
authors.forename: Niels
authors.initials: N
authors.lastname: Kruse
authors.forename: Walter J
authors.initials: WJ
authors.lastname: Schulz-Schaeffer
authors.forename: Michael G
authors.initials: MG
authors.lastname: Schlossmacher
authors.forename: Brit
authors.initials: B
authors.lastname: Mollenhauer
mesh_terms: D000818:Animals; D002021:Buffers; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000379:methods / Q000592:standards; D010300:Parkinson Disease / Q000134:cerebrospinal fluid*; D012015:Reference Standards; D015203:Reproducibility of Results; D051844:alpha-Synuclein / Q000134:cerebrospinal fluid*
chemical_list: D002021:Buffers; D051844:alpha-Synuclein