title: Extracting genomic DNA of foodstuff by polyamidoamine (PAMAM)-magnetite nanoparticles.
abstract: Although genetically modified (GM) food is becoming increasingly available, consumers are showing a growing awareness about the need to identify GM and non-GM foodstuff: the reliable identification of GM/non-GM food is therefore an important tool in the social, health and safety debates. The present research responds to this need (i) through developing a novel "single-pot" preparation of PAMAM magnetite nanoparticles (PMNPs) and by fully defining their specific characteristics; (ii) by demonstrating the capability of the PMNPs to isolate genomic DNA from different sample foods; and (iii) by experimentally demonstrating the identification of the isolated DNA by gel-electrophoresis, thus being capable of screening GM and non-GM food.
authors.affiliation: Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Fengxiang
authors.initials: F
authors.lastname: Qie
authors.forename: Guoxin
authors.initials: G
authors.lastname: Zhang
authors.forename: Jianxuan
authors.initials: J
authors.lastname: Hou
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Sun
authors.forename: Shi-zhong
authors.initials: SZ
authors.lastname: Luo
authors.forename: Tianwei
authors.initials: T
authors.lastname: Tan
mesh_terms: D005591:Chemical Fractionation / Q000379:methods*; D004247:DNA / Q000302:isolation & purification*; D050091:Dendrimers / Q000737:chemistry*; D030841:Food, Genetically Modified; D016678:Genome / Q000235:genetics*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D055672:Static Electricity; D013499:Surface Properties
chemical_list: D050091:Dendrimers; D058185:Magnetite Nanoparticles; C104700:PAMAM Starburst; D004247:DNA