title: Viral-induced encephalitis initiates distinct and functional CD103+ CD11b+ brain dendritic cell populations within the olfactory bulb.
abstract: Dendritic cells (DC) are antigen-presenting cells found in both lymphoid and nonlymphoid organs, including the brain (bDC) of Cd11c/eyfp transgenic C57BL/6 mice. Using an intranasal vesicular stomatitis virus infection, we demonstrated that EYFP(+) cells amass in areas associated with viral antigens, take on an activated morphology, and project their processes into infected neuronal tissue within the olfactory bulb. These bDC separated into three EYFP(+) CD45(+) CD11b(+) populations, all but one being able to functionally promote both T lymphocyte proliferation and T(H)1 cytokine production. One population was shown to emanate from the brain and a second population was peripherally derived. The third population was of indeterminate origin, being both radiosensitive and not replenished by donor bone marrow. Finally, each EYFP(+) population contained CD11b(+) CD103(+) subpopulations and could be distinguished in terms of CD115, Gr-1, and Ly-6C expression, highlighting mucosal and monocyte-derived DC lineages.
authors.affiliation: Laboratory of Neuroendocrinology, The Rockefeller University, New York, NY 10065, USA.
authors.forename: Paul M
authors.initials: PM
authors.lastname: D'Agostino
authors.forename: Changsoo
authors.initials: C
authors.lastname: Kwak
authors.forename: Haley A
authors.initials: HA
authors.lastname: Vecchiarelli
authors.forename: Judit Gal
authors.initials: JG
authors.lastname: Toth
authors.forename: James M
authors.initials: JM
authors.lastname: Miller
authors.forename: Zahrah
authors.initials: Z
authors.lastname: Masheeb
authors.forename: Bruce S
authors.initials: BS
authors.lastname: McEwen
authors.forename: Karen
authors.initials: K
authors.lastname: Bulloch
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000276:immunology; D015703:Antigens, CD / Q000276:immunology / Q000378:metabolism; D000950:Antigens, Ly / Q000276:immunology / Q000378:metabolism; D001921:Brain / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D039481:CD11b Antigen / Q000276:immunology / Q000378:metabolism; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D018792:Encephalitis, Viral / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D005434:Flow Cytometry; D039001:Integrin alpha Chains / Q000276:immunology / Q000378:metabolism; D017493:Leukocyte Common Antigens / Q000276:immunology / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D018613:Microscopy, Confocal; D009830:Olfactory Bulb / Q000276:immunology* / Q000378:metabolism; D010047:Ovalbumin / Q000276:immunology; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000276:immunology / Q000378:metabolism; D019707:Receptors, Chemokine / Q000276:immunology / Q000378:metabolism; D018353:Rhabdoviridae Infections / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D014721:Vesicular stomatitis Indiana virus / Q000276:immunology
chemical_list: D015703:Antigens, CD; D000950:Antigens, Ly; D039481:CD11b Antigen; C503133:Gr-1 protein, mouse; D039001:Integrin alpha Chains; D008164:Luminescent Proteins; C494192:Ly-6C antigen, mouse; D019707:Receptors, Chemokine; C091367:alpha E integrins; D010047:Ovalbumin; D016186:Receptor, Macrophage Colony-Stimulating Factor; D017493:Leukocyte Common Antigens; C516770:Ptprc protein, mouse