title: Designation of enzyme activity of glycine-N-acyltransferase family genes and depression of glycine-N-acyltransferase in human hepatocellular carcinoma.
abstract: The human glycine-N-acyltransferase (hGLYAT) gene and two related-genes (GLYATL1 and GLYATL2) were isolated. Human GLYAT, GLYATL1, and GLYATL2 cDNAs were isolated and shown to encode polypeptides of 295, 302, and 294 amino acids, respectively. GLYAT catalyzes glycine-N-acyltransfer reaction with benzoyl-CoA acting as a typical aralkyl transferase, while GLYATL1 catalyzed glutamine-N-acyltransfer reaction with phenylacetyl-CoA as an arylacetyl transferase. GLYAT was shown to be expressed specifically in the liver and kidney, and the cellular localization of GLYAT protein was restricted to the mitochondria. Interestingly, labeling using highly affinity purified anti-GLYAT antibody revealed that GLYAT expression was suppressed in all hepatocellular carcinomas, but not in other liver diseases. hGLYAT repression in cancerous cells in the liver was controlled at the transcriptional level. hGLYAT is a good candidate as a novel marker of hepatocellular carcinoma and may be a key molecule in the transition between differentiation and carcinogenesis of liver cells.
authors.affiliation: Department of Biomolecular Science, Faculty of Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan.
authors.forename: Moe
authors.initials: M
authors.lastname: Matsuo
authors.forename: Kensuke
authors.initials: K
authors.lastname: Terai
authors.forename: Noriaki
authors.initials: N
authors.lastname: Kameda
authors.forename: Aya
authors.initials: A
authors.lastname: Matsumoto
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Kurokawa
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Funase
authors.forename: Kazuko
authors.initials: K
authors.lastname: Nishikawa
authors.forename: Naoki
authors.initials: N
authors.lastname: Sugaya
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Hiruta
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Kishimoto
mesh_terms: D000214:Acyl Coenzyme A / Q000378:metabolism; D000217:Acyltransferases / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D006528:Carcinoma, Hepatocellular / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D045744:Cell Line, Tumor; D006801:Humans; D008113:Liver Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D008930:Mitochondria, Liver / Q000201:enzymology; D008969:Molecular Sequence Data
chemical_list: D000214:Acyl Coenzyme A; C037817:benzoyl-coenzyme A; D000217:Acyltransferases; C020680:glycine acyltransferase