title: Calmodulin-binding protein CBP60g is a positive regulator of both disease resistance and drought tolerance in Arabidopsis.
abstract: Calmodulin-binding proteins (CBPs) have been known to be involved in both biotic and abiotic stress responses. Recently, two closely related CBPs, Arabidopsis SAR Deficient 1 and CBP60g, were found to belong to a new family of transcription factors that regulate salicylic acid (SA) biosynthesis triggered by microbe-associated molecular patterns. In this study, we found that overexpression of CBP60g in Arabidopsis caused elevated SA accumulation, increased expression of the defense genes, and enhanced resistance to Pseudomonas syringae. In addition to the enhanced defense response, the CBP60g overexpression lines showed hypersensitivity to abscisic acid (ABA) and enhanced tolerance to drought stress. We also found that treatment with ABA and drought stress leads to a higher expression level of the ICS1 gene, which encodes isochorismate synthase, in the CBP60g overexpression lines than in the wild-type control plants. Our results suggest that CBP60g serves as a molecular link that positively regulates ABA- and SA-mediated pathways in plants.
  
  
  CONCLUSIONS
  Overexpression of CBP60g in Arabidopsis enhanced the defense response, hypersensitivity to abscisic acid and tolerance to drought stress.
authors.affiliation: College of Life Sciences, Inner Mongolia Agricultural University, Hohhot 010018, People's Republic of China.
authors.forename: Dongli
authors.initials: D
authors.lastname: Wan
authors.forename: Ruili
authors.initials: R
authors.lastname: Li
authors.forename: Bo
authors.initials: B
authors.lastname: Zou
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Jingyu
authors.initials: J
authors.lastname: Cong
authors.forename: Ruigang
authors.initials: R
authors.lastname: Wang
authors.forename: Yiji
authors.initials: Y
authors.lastname: Xia
authors.forename: Guojing
authors.initials: G
authors.lastname: Li
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002148:Calmodulin-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D060467:Disease Resistance; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D057865:Plant Immunity; D044224:Pseudomonas syringae / Q000472:pathogenicity; D020156:Salicylic Acid / Q000378:metabolism; D014170:Transformation, Genetic
chemical_list: D029681:Arabidopsis Proteins; C570511:CBP60g protein, Arabidopsis; D002148:Calmodulin-Binding Proteins; D000040:Abscisic Acid; D020156:Salicylic Acid