title: Bifidobacterium longum modified recombinant HU protein as a vector for nonviral delivery of DNA to HEK293 human cell culture.
abstract: Creation of effective nontoxic highly specific systems for nonviral transportation of DNA is one of the priority problems in the development of genotherapeutic methods. Chimerical recombinant proteins consisting of Antp and Tat protein cell-penetrating domains and Bifidobacterium longum DNA-binding histone-like protein HU were obtained. The resultant recombinant proteins bind to plasmid DNA in vitro and provide intracellular delivery and expression of the reporter genetic construction with GFP gene in cultured HEK293 human cells.
authors.affiliation: Russian State Medical University, Russian Ministry of Health Care and Social Development of Russian Federation, Moscow, Russia.
authors.forename: E V
authors.initials: EV
authors.lastname: Khokhlova
authors.forename: B A
authors.initials: BA
authors.lastname: Efimov
authors.forename: L I
authors.initials: LI
authors.lastname: Kafarskaia
authors.forename: K A
authors.initials: KA
authors.lastname: Pavlov
authors.forename: A N
authors.initials: AN
authors.lastname: Shkoporov
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001644:Bifidobacterium / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D005822:Genetic Vectors / Q000235:genetics*; D057809:HEK293 Cells; D006801:Humans; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins