title: Cloning, monoclonal antibody production, and bodily distribution pattern of a bovine lipocalin.
abstract: A bovine lipocalin, previously identified as a putative odorant-binding protein in bovine colostrum (bcOBP), was cloned and expressed, and its monoclonal antibody was established. bcOBP was constantly secreted into milk on day of parturition until at least 10 d postpartum at a concentration of 181±39 µg/L. Besides milk, bcOBP occurred in the nasal mucus, saliva, amniotic fluid, vaginal discharge, and blood plasma. Despite its low concentration, the distribution pattern and the finding that bcOBP harbored a characteristic sequence motif, CxxxC, which is conserved among insect and mammal pheromone binding proteins, suggest that bcOBP functions as a pheromone carrier. The presence of bcOBP in the plasma at varied concentrations depending on the lactation period does not exclude the possibility that bcOBP is secreted into milk from the blood. Cross-reactivity of the monoclonal antibody indicated presence of proteins homologous to bcOBP in the colostrum of farm animals of Cetartiodactyla.
authors.affiliation: Department of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan.
authors.forename: Tamar
authors.initials: T
authors.lastname: Japaridze
authors.forename: Akitsugu
authors.initials: A
authors.lastname: Senda
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Nozaki
authors.forename: Mayumi
authors.initials: M
authors.lastname: Yanagida
authors.forename: Takumi
authors.initials: T
authors.lastname: Suzuki
authors.forename: Khuukhenbaatar
authors.initials: K
authors.lastname: Ganzorig
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Kushi
authors.forename: Katsuya
authors.initials: K
authors.lastname: Kida
authors.forename: Tadasu
authors.initials: T
authors.lastname: Urashima
authors.forename: Rupert M
authors.initials: RM
authors.lastname: Bruckmaier
authors.forename: Kenji
authors.initials: K
authors.lastname: Fukuda
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D001826:Body Fluids / Q000737:chemistry; D002417:Cattle / Q000378:metabolism*; D003001:Cloning, Molecular; D003126:Colostrum / Q000737:chemistry*; D005260:Female; D007774:Lactation / Q000502:physiology; D054834:Lipocalins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008892:Milk / Q000737:chemistry; D008894:Milk Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D009928:Organ Specificity; D010675:Pheromones / Q000378:metabolism; D011247:Pregnancy; D013045:Species Specificity; D013997:Time Factors
chemical_list: D000911:Antibodies, Monoclonal; D054834:Lipocalins; D008894:Milk Proteins; D010675:Pheromones