title: A fragment of the LG3 peptide of endorepellin is present in the urine of physically active mining workers: a potential marker of physical activity.
abstract: Biomarker analysis has been implemented in sports research in an attempt to monitor the effects of exertion and fatigue in athletes. This study proposed that while such biomarkers may be useful for monitoring injury risk in workers, proteomic approaches might also be utilised to identify novel exertion or injury markers. We found that urinary urea and cortisol levels were significantly elevated in mining workers following a 12 hour overnight shift. These levels failed to return to baseline over 24 h in the more active maintenance crew compared to truck drivers (operators) suggesting a lack of recovery between shifts. Use of a SELDI-TOF MS approach to detect novel exertion or injury markers revealed a spectral feature which was associated with workers in both work categories who were engaged in higher levels of physical activity. This feature was identified as the LG3 peptide, a C-terminal fragment of the anti-angiogenic/anti-tumourigenic protein endorepellin. This finding suggests that urinary LG3 peptide may be a biomarker of physical activity. It is also possible that the activity mediated release of LG3/endorepellin into the circulation may represent a biological mechanism for the known inverse association between physical activity and cancer risk/survival.
authors.affiliation: Tissue Repair and Regeneration Program, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, Australia. a.parker@qut.edu.au
authors.forename: Tony J
authors.initials: TJ
authors.lastname: Parker
authors.forename: Dayle L
authors.initials: DL
authors.lastname: Sampson
authors.forename: Daniel
authors.initials: D
authors.lastname: Broszczak
authors.forename: Yee L
authors.initials: YL
authors.lastname: Chng
authors.forename: Shea L
authors.initials: SL
authors.lastname: Carter
authors.forename: David I
authors.initials: DI
authors.lastname: Leavesley
authors.forename: Anthony W
authors.initials: AW
authors.lastname: Parker
authors.forename: Zee
authors.initials: Z
authors.lastname: Upton
mesh_terms: D000328:Adult; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D019812:Heparan Sulfate Proteoglycans / Q000737:chemistry*; D006801:Humans; D006854:Hydrocortisone / Q000652:urine; D008297:Male; D008875:Middle Aged; D008906:Mining; D009043:Motor Activity; D016273:Occupational Exposure; D010446:Peptide Fragments / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D019812:Heparan Sulfate Proteoglycans; D010446:Peptide Fragments; C071927:perlecan; D006854:Hydrocortisone