title: Allelematch: an R package for identifying unique multilocus genotypes where genotyping error and missing data may be present.
abstract: We present allelematch, an R package, to automate the identification of unique multilocus genotypes in data sets where the number of individuals is unknown, and where genotyping error and missing data may be present. Such conditions commonly occur in noninvasive sampling protocols. Output from the software enables a comparison of unique genotypes and their matches, and facilitates the review of differences between profiles. The software has a variety of applications in molecular ecology, and may be valuable where a large number of samples must be processed, unique genotypes identified, and repeated observations made over space and time. We used simulations to assess the performance of allelematch and found that it can reliably and accurately determine the correct number of unique genotypes (± 3%) across a broad range of data set properties. We found that the software performs with highest accuracy when genotyping error is below 4%. The R package is available from the Comprehensive R Archive Network (http://cran.r-project.org/). Supplementary documentation and tutorials are provided.
authors.affiliation: Natural Resources Institute, University of Manitoba, 70 Dysart Road, Winnipeg, MB, Canada. pgalpern@gmail.com
authors.forename: Paul
authors.initials: P
authors.lastname: Galpern
authors.forename: Micheline
authors.initials: M
authors.lastname: Manseau
authors.forename: Peter
authors.initials: P
authors.lastname: Hettinga
authors.forename: Karen
authors.initials: K
authors.lastname: Smith
authors.forename: Paul
authors.initials: P
authors.lastname: Wilson
mesh_terms: D005838:Genotype; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D058885:Multilocus Sequence Typing / Q000379:methods*; D010363:Pattern Recognition, Automated; D017422:Sequence Analysis, DNA; D012984:Software