title: Expansion of CD14+CD16+ monocytes producing TNF-α in complication-free diabetes type 1 juvenile onset patients.
abstract: We concentrated on the complication-free phase of juvenile onset type 1 diabetes mellitus (T1DM) searching for associations between concentration of inflammatory factors TNF-α, CRP and VEGF and two monocyte subsets the CD14(++)CD16(-) and CD14(+)CD16(+). We analysed a randomly selected group of 150 patients without complications (disease duration 2.74 ± 2.51 years) at the start of the project and 5 years later. They were compared with 24 patients with retinopathy (6.53 ± 3.39 years of disease) and 30 healthy volunteers. Our results indicate that in the complication-free period the concentration of TNF-α significantly increased and continued to increase after retinopathy was established. After 5 years the percentage and absolute number of CD14(+)CD16(+) monocytes doubled in complication-free patients. Our study indicates that the size of CD14(+)CD16(+) monocyte subset may be used alternatively to CRP values as an indicator of inflammation grade. Our results imply the necessity of trials using anti-TNF-α therapy in the complication-free phase of the disease.
authors.affiliation: Department of Immunology, Medical University of Gdańsk, Gdańs, Poland. jolmys@gumed.edu.pl
authors.forename: Jolanta
authors.initials: J
authors.lastname: Myśliwska
authors.forename: Marcin
authors.initials: M
authors.lastname: Smardzewski
authors.forename: Natalia
authors.initials: N
authors.lastname: Marek-Trzonkowska
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Myśliwiec
authors.forename: Krystyna
authors.initials: K
authors.lastname: Raczyńska
mesh_terms: D000293:Adolescent; D002097:C-Reactive Protein / Q000276:immunology / Q000378:metabolism; D049109:Cell Proliferation; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000276:immunology*; D003930:Diabetic Retinopathy / Q000097:blood / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000097:blood / Q000276:immunology; D006801:Humans; D018836:Inflammation Mediators / Q000276:immunology / Q000378:metabolism; D018950:Lipopolysaccharide Receptors / Q000097:blood / Q000276:immunology; D008297:Male; D009000:Monocytes / Q000276:immunology* / Q000378:metabolism; D017452:Receptors, IgG / Q000097:blood / Q000276:immunology; D012044:Regression Analysis; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000276:immunology*; D042461:Vascular Endothelial Growth Factor A / Q000097:blood / Q000276:immunology; D055815:Young Adult
chemical_list: D006684:HLA-DR Antigens; D018836:Inflammation Mediators; D018950:Lipopolysaccharide Receptors; D017452:Receptors, IgG; D014409:Tumor Necrosis Factor-alpha; D042461:Vascular Endothelial Growth Factor A; D002097:C-Reactive Protein