title: Injectable and thermo-sensitive PEG-PCL-PEG copolymer/collagen/n-HA hydrogel composite for guided bone regeneration.
abstract: A novel three-component biomimetic hydrogel composite was successfully prepared in this study, which was composed of triblock PEG-PCL-PEG copolymer (PECE), collagen and nano-hydroxyapatite (n-HA). The microstructure and thermo-responsibility of the obtained PECE/Collagen/n-HA hydrogel composite were characterized. Scanning electronic microscopy (SEM) showed that the composite exhibited an interconnected porous structure. The rheological analysis revealed that the composite existed good thermo-sensitivity. In vivo biocompatibility and biodegradability was investigated by implanting the hydrogel composite in muscle pouches of rats for 3, 7, and 14 days. Moreover, the osteogenic capacity was evaluated by means of implanting the composite material in cranial defects of New Zealand White rabbits for 4, 12 and 20 weeks. In vivo performances confirmed that the biodegradable PECE/Collagen/n-HA hydrogel composite had good biocompatibility and better performance in guided bone regeneration than the self-healing process. Thus the thermal-response PECE/Collagen/n-HA hydrogel composite had the great potential in bone tissue engineering.
authors.affiliation: State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, China.
authors.forename: ShaoZhi
authors.initials: S
authors.lastname: Fu
authors.forename: PeiYan
authors.initials: P
authors.lastname: Ni
authors.forename: BeiYu
authors.initials: B
authors.lastname: Wang
authors.forename: BingYang
authors.initials: B
authors.lastname: Chu
authors.forename: Lan
authors.initials: L
authors.lastname: Zheng
authors.forename: Feng
authors.initials: F
authors.lastname: Luo
authors.forename: JingCong
authors.initials: J
authors.lastname: Luo
authors.forename: ZhiYong
authors.initials: Z
authors.lastname: Qian
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000502:physiology*; D003094:Collagen / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D007267:Injections; D008855:Microscopy, Electron, Scanning; D053761:Nanocomposites / Q000737:chemistry / Q000648:ultrastructure; D011091:Polyesters / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods; D055114:X-Ray Microtomography
chemical_list: D011091:Polyesters; C502908:poly(ethylene glycol)-poly(caprolactone)-poly(ethylene glycol); D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011092:Polyethylene Glycols; D003094:Collagen; D017886:Durapatite