title: Plant proteins, minerals and trace elements of Eurycoma longifolia (Tongkat Ali).
abstract: A water extraction method has been used to extract plant proteins from the roots of Eurycoma longifolia harvested from Perak and Pahang, Malaysia. On the basis of the spectroscopic Bradford assay, Tongkat Ali Perak and Pahang contained 0.3868 and 0.9573 mg mL(-1) of crude protein, respectively. The crude proteins were separated by one dimensional 15% sodium dodecyl sulphate polyacrylamide gel electrophoresis into two (49.8 and 5.5 kD) and four (49.8, 24.7, 21.1 and 5.5 kD) protein spots for Tongkat Ali Perak and Pahang, respectively. Isoleucine was present in the highest concentration significantly. Both plant samples showed differences in the mineral and trace element profiles, but the minerals calcium, magnesium and potassium were present in the highest concentration. The highly concerned toxic metals such as arsenic and lead were not detected.
authors.affiliation: Metabolites Profiling Laboratory, Institute of Bioproduct Development, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor, Malaysia. lschua@ibd.utm.my
authors.forename: Lee Suan
authors.initials: LS
authors.lastname: Chua
authors.forename: Nurulaini
authors.initials: N
authors.lastname: Abdul-Rahman
authors.forename: Bustanur
authors.initials: B
authors.lastname: Rosidi
authors.forename: Chew Tin
authors.initials: CT
authors.lastname: Lee
mesh_terms: D002118:Calcium / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D035701:Eurycoma / Q000737:chemistry*; D008274:Magnesium / Q000032:analysis; D008903:Minerals / Q000032:analysis*; D010940:Plant Proteins / Q000032:analysis*; D011188:Potassium / Q000032:analysis; D014131:Trace Elements / Q000032:analysis*
chemical_list: D008903:Minerals; D010940:Plant Proteins; D014131:Trace Elements; D008274:Magnesium; D011188:Potassium; D002118:Calcium