title: Architecture of the spliceosome.
abstract: Precursor-mRNA splicing is catalyzed by an extraordinarily large and highly dynamic macromolecular assemblage termed the spliceosome. Detailed biochemical and structural study of the spliceosome presents a formidable challenge, but there has recently been significant progress made on this front highlighted by the crystal structure of a 10-subunit human U1 snRNP. This review provides an overview of our current understanding of the architecture of the spliceosome and the RNA-protein complexes integral to its function, the U snRNPs.
authors.affiliation: Department of Biochemistry, Brandeis University, 415 South Street, Waltham, MA 02454, USA.
authors.forename: Clarisse
authors.initials: C
authors.lastname: van der Feltz
authors.forename: Kelsey
authors.initials: K
authors.lastname: Anthony
authors.forename: Axel
authors.initials: A
authors.lastname: Brilot
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Pomeranz Krummel
mesh_terms: D000818:Animals; D006801:Humans; D008958:Models, Molecular; D011487:Protein Conformation; D012322:RNA Precursors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012326:RNA Splicing; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017412:Ribonucleoprotein, U1 Small Nuclear / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017381:Spliceosomes / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D012322:RNA Precursors; D012333:RNA, Messenger; D017412:Ribonucleoprotein, U1 Small Nuclear