title: Eriodictyol prevents early retinal and plasma abnormalities in streptozotocin-induced diabetic rats.
abstract: Diabetic retinopathy is a complex disease that has potential involvement of inflammatory and oxidative stress-related pathways in its pathogenesis. We hypothesized that eriodictyol, one of the most abundant dietary flavonoids, could be effective against diabetic retinopathy, which involves significant oxidative stress and inflammation. The aim of the present study was to investigate the effects of eriodictyol in early retinal and plasma changes of streptozotocin-induced diabetic rats. The effect of eriodictyol treatment (0.1, 1, 10 mg/kg daily for 10 days) was evaluated by TNF-α, ICAM-1, VEGF, and eNOS protein levels measurement in the retina, plasma lipid peroxidation, and blood-retinal barrier (BRB) integrity. Increased amounts of cytokines, adhesion molecule, and nitric oxide synthase were observed in retina from diabetic rats. Eriodictyol treatment significantly lowered retinal TNF-α, ICAM-1, VEGF, and eNOS in a dose-dependent manner. Further, treatment with eriodictyol significantly suppressed diabetes-related lipid peroxidation, as well as the BRB breakdown. These data demonstrated that eriodictyol attenuates the degree of retinal inflammation and plasma lipid peroxidation preserving the BRB in early diabetic rats.
authors.affiliation: Department of Clinical and Molecular Biomedicine, Section of Pharmacology and Biochemistry, Medical School, University of Catania, Catania, Italy. claudio.bucolo@unict.it
authors.forename: Claudio
authors.initials: C
authors.lastname: Bucolo
authors.forename: Gian Marco
authors.initials: GM
authors.lastname: Leggio
authors.forename: Filippo
authors.initials: F
authors.lastname: Drago
authors.forename: Salvatore
authors.initials: S
authors.lastname: Salomone
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000737:chemistry / Q000627:therapeutic use*; D001813:Blood-Retinal Barrier / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000150:complications / Q000188:drug therapy* / Q000276:immunology; D003930:Diabetic Retinopathy / Q000097:blood / Q000209:etiology / Q000276:immunology / Q000517:prevention & control*; D004305:Dose-Response Relationship, Drug; D044950:Flavanones / Q000008:administration & dosage / Q000737:chemistry / Q000627:therapeutic use*; D015227:Lipid Peroxidation / Q000187:drug effects; D008054:Lipid Peroxides / Q000097:blood*; D008297:Male; D015394:Molecular Structure; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000187:drug effects / Q000276:immunology / Q000378:metabolism
chemical_list: D000975:Antioxidants; D044950:Flavanones; D008054:Lipid Peroxides; C007619:eriodictyol