title: Development of a sequence typing scheme for differentiation of  Salmonella  Enteritidis strains.
abstract: A DNA sequence typing scheme based on the caiC and SEN0629 loci was developed for differentiation of Salmonella Enteritidis strains and validated using a diverse collection of 102 isolates representing 38 phage types from different sources, year of isolation, geographical locations and epidemiological backgrounds. caiC encodes a probable crotonobetaine/carnitine-CoA ligase, and SEN0629 is a pseudogene. Our system allowed for discrimination of 16 sequence types (STs) among the 102 isolates analysed and intraphage type differentiation. Our findings also suggested that the stability of phage typing may be adversely affected by the occurrence of phage type conversion events. During a confirmatory phage typing analysis performed by a reference laboratory, 13 of 31 S.  Enteritidis strains representing nine phage types were assigned phage types that differed from the ones originally determined by the same reference laboratory. It is possible that this phenomenon passes largely unrecognized in reference laboratories performing routine phage typing analyses. Our results demonstrate that phage typing is an unstable system displaying limited reproducibility and that the two-loci sequence typing scheme is highly discriminatory, stable, truly portable and has the potential to become the new gold standard for epidemiological typing of S . Enteritidis strains.
authors.affiliation: California Animal Health and Food Safety Laboratory System, School of Veterinary Medicine, University of California Davis, Davis, CA, USA.
authors.forename: Bertrand
authors.initials: B
authors.lastname: Tankouo-Sandjong
authors.forename: Hailu
authors.initials: H
authors.lastname: Kinde
authors.forename: Isha
authors.initials: I
authors.lastname: Wallace
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques / Q000379:methods*; D001434:Bacteriophage Typing / Q000379:methods; D003066:Coenzyme A Ligases / Q000737:chemistry / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing / Q000379:methods*; D011544:Pseudogenes / Q000235:genetics*; D015203:Reproducibility of Results; D012480:Salmonella Infections / Q000453:epidemiology / Q000382:microbiology; D012477:Salmonella enteritidis / Q000145:classification* / Q000235:genetics* / Q000821:virology; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D003066:Coenzyme A Ligases