title: Activation of acetylcholinesterase after U-74389G administration in a porcine model of intracerebral hemorrhage.
abstract: Spontaneous intracerebral hemorrhage (ICH) accounts for 10-15% of all strokes. Despite high incidence, morbidity and mortality, the precise pathophysiology of spontaneous ICH is not fully understood, while there is little data concerning the mechanisms that follow the primary insult of the hematoma formation. The cholinergic system, apart from its colossal importance as a neurotransmission system, seems to also play an important role in brain injury recovery. It has been recently suggested that the brain possesses a cholinergic anti-inflammatory pathway that counteracts the inflammatory responses after ICH, thereby limiting damage to the brain itself. We, herein, report the findings of our study concerning the role of acetylcholinesterase (AChE; a crucial membrane-bound enzyme involved in cholinergic neurotransmission) in a porcine model of spontaneous ICH, with a focus on the first 4 and 24 h following the lesion's induction, in combination with a study of the effectiveness of the lazaroid antioxidant U-74389G administration. Our study demonstrates the activation of AChE activity following U-74389G administration. The lazaroid U-74389G seems to be an established neuroprotectant and this is the first report of its supporting role in the enhancement of cholinergic response to the induction of ICH.
authors.affiliation: Department of Pharmacology, Medical School, National and Kapodistrian University of Athens, Greece.
authors.forename: Alexios
authors.initials: A
authors.lastname: Bimpis
authors.forename: Apostolos
authors.initials: A
authors.lastname: Papalois
authors.forename: Stylianos
authors.initials: S
authors.lastname: Tsakiris
authors.forename: Apostolos
authors.initials: A
authors.lastname: Zarros
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Kalafatakis
authors.forename: John
authors.initials: J
authors.lastname: Botis
authors.forename: Vasileios
authors.initials: V
authors.lastname: Stolakis
authors.forename: Konstantinos M
authors.initials: KM
authors.lastname: Zissis
authors.forename: Charis
authors.initials: C
authors.lastname: Liapi
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001479:Basal Ganglia / Q000201:enzymology / Q000473:pathology; D002540:Cerebral Cortex / Q000201:enzymology / Q000473:pathology; D002543:Cerebral Hemorrhage / Q000201:enzymology* / Q000473:pathology; D004789:Enzyme Activation / Q000187:drug effects*; D007839:Functional Laterality / Q000502:physiology; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology*; D011281:Pregnatrienes / Q000494:pharmacology*; D013552:Swine
chemical_list: D000975:Antioxidants; D018696:Neuroprotective Agents; D011281:Pregnatrienes; C074965:U 74389F; D000110:Acetylcholinesterase