title: Tiam1, negatively regulated by miR-22, miR-183 and miR-31, is involved             in migration, invasion and viability of ovarian cancer cells.
abstract: Tiam1 has been implicated in the invasive phenotype of various carcinomas.             However, its role in ovarian cancer remains to be elucidated, including its upstream             regulatory mechanisms. In the present study, we examined the differential expression             of Tiam1 in 10 normal ovarian tissues and 17 paired primary and corresponding             metastatic ovarian cancer tissues by semi-quantitative immunohistochemistry. It             was found that Tiam1 expression was remarkably increased in both primary and metastatic             ovarian cancer tissues relative to normal ovarian tissues. Loss-of-function study             revealed that downregulation of Tiam1 in SKOV-3ip and HO-8910PM cells lead to             reduced cell migration and invasion, and growth inhibition without significantly             affecting cell apoptosis. Subsequent regulatory study further confirmed the negative             regulatory effects of miR-22, miR-183 and miR-31 on Tiam1 expression. Taken together,             our data suggested that Tiam1 may be involved in the aggressive behavior of ovarian             cancer, and differential expression profiles of microRNA (miRNA) may contribute             to the dysregulation of Tiam1 abundance, which contributes to the invasive, migratory             and viability properties of ovarian cancer cells.
authors.affiliation: Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Shanhui
authors.initials: S
authors.lastname: Liang
authors.forename: Hongyan
authors.initials: H
authors.lastname: Jin
authors.forename: Congjian
authors.initials: C
authors.lastname: Xu
authors.forename: Duan
authors.initials: D
authors.lastname: Ma
authors.forename: Xin
authors.initials: X
authors.lastname: Lu
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D002470:Cell Survival; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D020662:Guanine Nucleotide Exchange Factors / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness; D010051:Ovarian Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D010053:Ovary / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering; D000075684:T-Lymphoma Invasion and Metastasis-inducing Protein 1
chemical_list: D020662:Guanine Nucleotide Exchange Factors; C533449:MIRN183 microRNA, human; C548375:MIRN22 microRNA, human; C534612:MIRN31 microRNA, human; D035683:MicroRNAs; D034741:RNA, Small Interfering; D000075684:T-Lymphoma Invasion and Metastasis-inducing Protein 1; C087334:TIAM1 protein, human