title: Laser-capture microdissection and transcriptional profiling of the dorsomedial nucleus of the hypothalamus.
abstract: Identifying neuronal molecular markers with restricted patterns of expression is a crucial step in dissecting the numerous pathways and functions of the brain. While the dorsomedial nucleus of the hypothalamus (DMH) has been implicated in a host of physiological processes, current functional studies have been limited by the lack of molecular markers specific for DMH. Identification of such markers would facilitate the development of mouse models with DMH-specific genetic manipulations. Here we used a combination of laser-capture microdissection (LCM) and gene expression profiling to identify genes that are highly expressed within the DMH relative to adjacent hypothalamic regions. Six of the most highly expressed of these genes, Gpr50, 4930511J11Rik, Pcsk5, Grp, Sulf1, and Rorβ, were further characterized by real-time polymerase chain reaction (PCR) analysis and in situ hybridization histochemistry. The genes identified in this article will provide the basis for future gene-targeted approaches for studying DMH function.
authors.affiliation: Department of Internal Medicine and Department of Pharmacology, Division of Hypothalamic Research, University of Texas Southwestern Medical Center, Dallas, Texas 75390-9077, USA.
authors.forename: Syann
authors.initials: S
authors.lastname: Lee
authors.forename: Angie L
authors.initials: AL
authors.lastname: Bookout
authors.forename: Charlotte E
authors.initials: CE
authors.lastname: Lee
authors.forename: Laurent
authors.initials: L
authors.lastname: Gautron
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Harper
authors.forename: Carol F
authors.initials: CF
authors.lastname: Elias
authors.forename: Bradford B
authors.initials: BB
authors.lastname: Lowell
authors.forename: Joel K
authors.initials: JK
authors.lastname: Elmquist
mesh_terms: D000818:Animals; D004302:Dorsomedial Hypothalamic Nucleus / Q000378:metabolism*; D020869:Gene Expression Profiling; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D061346:Laser Capture Microdissection; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger