title: Antimicrobial peptides from Adenanthera pavonina L. seeds: characterization and antifungal activity.
abstract: In this study, the antifungal activity of peptides extracted from Adenanthera pavonina seeds was assessed. Peptides were extracted and fractionated by DEAE-Sepharose chromatography. The non-retained D1 fraction efficiently inhibited the growth of the pathogenic fungi. This fraction was later further fractionated by reversed-phase chromatography, resulting in 23 sub-fractions. All separation processes were monitored by tricine-SDS-PAGE. Fractions H11 and H22 strongly inhibited the growth of Saccharomyces cerevisiae and Candida albicans. Fraction H11 caused 100% death in S. cerevisiae in an antimicrobial assay. The complete amino acid sequence of the peptide in fraction P2 was determined, revealing homology to plant defensins, which was named ApDef1. Peptides from fraction H22 were also sequenced.
authors.affiliation: Laboratorio de Fisiologia e Bioquimica de Microrganismos, Centro de Biociencias e Biotecnologia, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, 28013-602, RJ, Brazil.
authors.forename: Julia Ribeiro
authors.initials: JR
authors.lastname: Soares
authors.forename: Andre
authors.initials: A
authors.lastname: de Oliveira Carvalho
authors.forename: Izabela Silva
authors.initials: IS
authors.lastname: Dos Santos
authors.forename: Olga Lima Tavares
authors.initials: OL
authors.lastname: Machado
authors.forename: Viviane V
authors.initials: VV
authors.lastname: Nascimento
authors.forename: Ilka Maria
authors.initials: IM
authors.lastname: Vasconcelos
authors.forename: Andre Teixeira
authors.initials: AT
authors.lastname: da Silva Ferreira
authors.forename: Jonas Enrique
authors.initials: JE
authors.lastname: de Aguilar Perales
authors.forename: Valdirene Moreira
authors.initials: VM
authors.lastname: Gomes
mesh_terms: D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000494:pharmacology*; D002853:Chromatography, Liquid; D007887:Fabaceae / Q000737:chemistry*; D005658:Fungi / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D008969:Molecular Sequence Data; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry*; D016415:Sequence Alignment
chemical_list: D000935:Antifungal Agents; D023181:Antimicrobial Cationic Peptides; D010936:Plant Extracts