title: Hepatic hyperplasia and damages induces by zearalenone Fusarium mycotoxins in BALB/c mice.
abstract: BACKGROUND
  Zearalenone is a mycoestrogen and considered a mycotoxin.
  
  
  OBJECTIVE
  To establish whether zearalenone produced hepatotoxicity via oral administration.
  
  
  METHODS
  Zearalenone was orally administered at a dose of 50 mg, 100 mg and 200 mg ZEN/body weight/daily, respectively, for 14 days to three groups of BALB/c mice. Diagnostic modalities used to evaluate hepatic damage and impaired hepatic function pre- and post zearalenone administration included hepatic marker enzyme activity, pentobarbital sleeping time, cytochrome P-(450) activities and histopathologic evaluation of liver.
  
  
  RESULTS
  Significant histopathologic changes viz. sinusoidal congestion, cytoplasmic vacuolization, hepatocellular necrosis and neutrophil infiltration were observed after evaluating of liver section from each group after accumulated zearalenone exposure. Further, zearalenone exposure increased activities of alanine transaminase, aspartate transaminase and lipid peroxides whereas activities of tissue glutathione and cytochrome P(450) were decreased as compared to control mice. Zearalenone also increased the sleeping time and decreased sleeping latency after pentobarbital through intraperitoneal route as compared to control mice which indicates that the impairment of hepatic metabolizing enzymes by zearalenone.
  
  
  CONCLUSIONS
  Zearalenone is a potential hepatotoxin by oral route.
authors.affiliation: Division of Pharmaceutical Technology, Defence Research Laboratory, India. chattopadhyay.drdo@gmail.com
authors.forename: Pronobesh
authors.initials: P
authors.lastname: Chatopadhyay
authors.forename: Anurag
authors.initials: A
authors.lastname: Pandey
authors.forename: Asshwani K
authors.initials: AK
authors.lastname: Chaurasia
authors.forename: Addesh
authors.initials: A
authors.lastname: Upadhyay
authors.forename: Sanjev
authors.initials: S
authors.lastname: Karmakar
authors.forename: Lokendra
authors.initials: L
authors.lastname: Singh
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D003577:Cytochrome P-450 Enzyme System / Q000097:blood; D005670:Fusarium / Q000737:chemistry*; D006965:Hyperplasia / Q000139:chemically induced / Q000473:pathology; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009183:Mycotoxins / Q000008:administration & dosage / Q000633:toxicity*; D015025:Zearalenone / Q000008:administration & dosage / Q000633:toxicity*
chemical_list: D009183:Mycotoxins; D015025:Zearalenone; D003577:Cytochrome P-450 Enzyme System; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase