title: Noble gas (argon and xenon)-saturated cold storage solutions reduce ischemia-reperfusion injury in a rat model of renal transplantation.
abstract: BACKGROUND
  Following kidney transplantation, ischemia-reperfusion injury contributes to adverse outcomes. The purpose of this study was to determine whether a cold-storage solution saturated with noble gas (xenon or argon) could limit ischemia-reperfusion injury following cold ischemia.
  
  
  METHODS
  Sixty Wistar rats were randomly allocated to 4 experimental groups. Kidneys were harvested and then stored for 6 h before transplantation in cold-storage solution (Celsior®) saturated with either air, nitrogen, xenon or argon. A syngenic orthotopic transplantation was performed. Renal function was determined on days 7 and 14 after transplantation. Transplanted kidneys were removed on day 14 for histological and immunohistochemical analyses.
  
  
  RESULTS
  Creatinine clearance was significantly higher and urinary albumin significantly lower in the argon and xenon groups than in the other groups at days 7 and 14. These effects were considerably more pronounced for argon than for xenon. In addition, kidneys stored with argon, and to a lesser extent those stored with xenon, displayed preserved renal architecture as well as higher CD-10 and little active caspase-3 expression compared to other groups.
  
  
  CONCLUSIONS
  Argon- or xenon-satured cold-storage solution preserved renal architecture and function following transplantation by reducing ischemia-reperfusion injury.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Jouy-en-Josas, France.
authors.forename: Y
authors.initials: Y
authors.lastname: Irani
authors.forename: J L
authors.initials: JL
authors.lastname: Pype
authors.forename: A R
authors.initials: AR
authors.lastname: Martin
authors.forename: C F
authors.initials: CF
authors.lastname: Chong
authors.forename: L
authors.initials: L
authors.lastname: Daniel
authors.forename: J
authors.initials: J
authors.lastname: Gaudart
authors.forename: Z
authors.initials: Z
authors.lastname: Ibrahim
authors.forename: G
authors.initials: G
authors.lastname: Magalon
authors.forename: M
authors.initials: M
authors.lastname: Lemaire
authors.forename: J
authors.initials: J
authors.lastname: Hardwigsen