title: Novel route for rapid biosynthesis of copper nanoparticles using aqueous extract of Calotropis procera L. latex and their cytotoxicity on tumor cells.
abstract: This paper accounts for novel, low-cost, eco-friendly route for rapid biosynthesis of copper nanoparticles. Cysteine proteases present in the latex of Calotropis procera L. were used to fabricate copper nanoparticles from copper acetate. Copper nanoparticles were initially characterized by transmission electron microscopy (TEM) and X-ray diffraction technique (XRD). Transmission electron microscopy (TEM) was used to estimate the size and shape of nanoparticles. The average size of copper nanoparticles was found to be 15 ± 1.7 nm. Energy dispersive analysis of X-rays (EDAX) showed distinct peaks of copper. Fourier transform infrared spectroscopy (FTIR) was performed to confirm capping behavior of the latex proteins that contributed to long term stability of copper nanoparticles (6 months) in aqueous medium. Copper nanoparticles synthesized by above method were monodisperse type. Cytotoxicity studies of latex stabilized copper nanoparticles were carried out on HeLa, A549 and BHK21 cell lines by MTT dye conversion assay. HeLa, A549 and BHK21 cells showed excellent viability even at 120 μM concentration of copper nanoparticles. This shows that copper nanoparticles synthesized by above method hold excellent biocompatibility.
authors.affiliation: Department of Microbiology, University of Pune, Pune 411007, Maharashtra, India.
authors.forename: Shrikant
authors.initials: S
authors.lastname: Harne
authors.forename: Ashwinikumar
authors.initials: A
authors.lastname: Sharma
authors.forename: Mayur
authors.initials: M
authors.lastname: Dhaygude
authors.forename: Shreeram
authors.initials: S
authors.lastname: Joglekar
authors.forename: Kisan
authors.initials: K
authors.lastname: Kodam
authors.forename: Manish
authors.initials: M
authors.lastname: Hudlikar
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D031107:Calotropis / Q000737:chemistry*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003300:Copper / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D006367:HeLa Cells; D006801:Humans; D007840:Latex / Q000737:chemistry / Q000378:metabolism*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D010316:Particle Size; D010936:Plant Extracts / Q000737:chemistry / Q000378:metabolism*; D013329:Structure-Activity Relationship; D013499:Surface Properties; D014867:Water / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D007840:Latex; D010936:Plant Extracts; D014867:Water; D003300:Copper