title: Interaction between endothelial nitric oxide synthase gene polymorphisms (-786T>C, 894G>T and intron 4 a/b) and cardiovascular risk factors in acute coronary syndromes.
abstract: OBJECTIVE
  Endothelial rupture of coronary plaque can represent the pathomorphological substratum of acute coronary syndrome (ACS). Polymorphisms in the NOS3 gene (eNOS) -786T>C, 894G>T and intron 4 a/b VNTR can be associated with a higher susceptibility for ACS. The present study is focused on the investigation of the interaction of these polymorphisms and cardiovascular risk factors in 135 patients with ACS and 115 control subjects.
  
  
  METHODS
  Case-control study where the allele and genotype frequencies of the polymorphisms -786T> C, 894G> T and intron 4 VNTR of the gene encoding eNOS were determined by PCR-RFLP associated with cardiovascular risk factors.
  
  
  RESULTS
  An association of the 894TT genotype and 894GT+GG (OR 1.4; 95% CI 1.0-1.8) in ACS has been observed. Subjects without dyslipidemia and intron 4 a/b genotype present a lower chance for ACS development, whereas subjects without diabetes and 894TT genotype show a higher risk for ACS (OR 1.7; 95% CI 1.2-2.3). In patients without dyslipidemia, the 894GG genotype presented a tendency to behave as a protector factor against ACS. Also, the 894GG genotype has been a protective factor for ACS in females (OR 0.5; CI 95% 0.2-0.9).
  
  
  CONCLUSIONS
  Our results suggest that eNOS polymorphisms may be an additional risk factor in development of ACS.
authors.affiliation: Universidade Federal do Pampa, Campus Uruguaiana-RS, Brazil. jcepiccoli@yahoo.com.br
authors.forename: Jacqueline
authors.initials: J
authors.lastname: da Costa Escobar Piccoli
authors.forename: Vanusa
authors.initials: V
authors.lastname: Manfredini
authors.forename: Fernanda Irma
authors.initials: FI
authors.lastname: Hamester
authors.forename: Josiane Bettim
authors.initials: JB
authors.lastname: Bandinelli
authors.forename: Ilan Maltz
authors.initials: IM
authors.lastname: Turkienicz
authors.forename: José Artur Bogo
authors.initials: JA
authors.lastname: Chies
authors.forename: Alessandra
authors.initials: A
authors.lastname: Peres
authors.forename: Luiz Carlos
authors.initials: LC
authors.lastname: Bodanese
authors.forename: Maurício Reis
authors.initials: MR
authors.lastname: Bogo
mesh_terms: D054058:Acute Coronary Syndrome / Q000201:enzymology / Q000235:genetics*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D016022:Case-Control Studies; D003920:Diabetes Mellitus / Q000201:enzymology / Q000235:genetics; D050171:Dyslipidemias / Q000201:enzymology / Q000235:genetics; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D007438:Introns; D008297:Male; D008875:Middle Aged; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide
chemical_list: C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III