title: Characterization of laccase isoforms produced by Pleurotus ostreatus in solid state fermentation of sugarcane bagasse.
abstract: Laccases are oxidative enzymes linked to biological degradation of lignin. The aim of this work was to evaluate the effect of inducers and different concentrations of nitrogen on production level of total laccase activity and pattern of laccase isoforms, produced in solid state fermentation of sugarcane bagasse by a selected strain of Pleurotus ostreatus. The addition of yeast extract 5 g/L, copper sulfate 150 μM and ferulic acid 2 mM provided highest enzymatic activity (167 U/g) and zymograms indicated the presence of six laccase isoforms (POXA1b, POXA3, POXC and three other isoforms). Results of protein identification by mass spectrometry confirmed the presence of POXC and POXA3 as the main isoenzymes, and also identified a glyoxal oxidase and three galactose oxidases. The fact that the isoenzyme POXA1b was not identified in the analyzed samples can be possibly explained by its sensitivity to protease degradation.
authors.affiliation: Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná, Coronel Francisco H. dos Santos Avenue, 210, Zip Code 81531-990 Curitiba, Brazil.
authors.forename: Susan Grace
authors.initials: SG
authors.lastname: Karp
authors.forename: Vincenza
authors.initials: V
authors.lastname: Faraco
authors.forename: Antonella
authors.initials: A
authors.lastname: Amore
authors.forename: Leila
authors.initials: L
authors.lastname: Birolo
authors.forename: Chiara
authors.initials: C
authors.lastname: Giangrande
authors.forename: Vanete Thomaz
authors.initials: VT
authors.lastname: Soccol
authors.forename: Ashok
authors.initials: A
authors.lastname: Pandey
authors.forename: Carlos Ricardo
authors.initials: CR
authors.lastname: Soccol
mesh_terms: D002482:Cellulose / Q000378:metabolism*; D005285:Fermentation; D042845:Laccase / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification; D020076:Pleurotus / Q000201:enzymology*; D020033:Protein Isoforms / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D031786:Saccharum / Q000382:microbiology*
chemical_list: D020033:Protein Isoforms; D002482:Cellulose; C027433:bagasse; D042845:Laccase