title: Genotype profiles of Mycobacterium avium subspecies paratuberculosis recovered from suspected and Crohn's disease patients in India.
abstract: Present study aimed to genotype Mycobacterium avium subspecies paratuberculosis (MAP) recovered from suspected and Crohn' s disease patients. A total of 32 MAP and DNA (directly from clinical samples) recovered from human origin were genotyped using IS 1311 PCR-REA. Isolates were cultured from stool, biopsies and blood clots of Crohn's disease patients, and stool samples of suspected (animal attendants, lab workers etc). Of the 32 MAP isolates belonging to 28 human beings, majority (84.3%) were genotyped as 'Bison type', while 21.7% were of 'cattle' and none was 'sheep' genotype. Study first time reports distribution of 'Cattle' and 'Bison type' 'genotypes in suspected and Crohn's patients on pilot scale in India. 'Bison type' genotype was predominant in the surveyed human population.
authors.affiliation: Veterinary Microbiology Laboratory, Animal Health Division, Central Institute Makhdoom, PO - Farah, District Mathura-281122 (UP), India. shoorvir.singh@gmail.com
authors.forename: A V
authors.initials: AV
authors.lastname: Singh
authors.forename: S V
authors.initials: SV
authors.lastname: Singh
authors.forename: J S
authors.initials: JS
authors.lastname: Sohal
authors.forename: P K
authors.initials: PK
authors.lastname: Singh
mesh_terms: D000818:Animals; D003424:Crohn Disease / Q000382:microbiology*; D005838:Genotype; D006041:Goats / Q000382:microbiology; D006801:Humans; D007194:India; D016927:Mycobacterium avium subsp. paratuberculosis / Q000145:classification* / Q000235:genetics; D010865:Pilot Projects; D000091402:Prohibitins; D012756:Sheep / Q000382:microbiology