title: Probing neuropeptide signaling at the organ and cellular domains via imaging mass spectrometry.
abstract: Imaging mass spectrometry (IMS) has evolved to be a promising technology due to its ability to detect a broad mass range of molecular species and create density maps for selected compounds. It is currently one of the most useful techniques to determine the spatial distribution of neuropeptides in cells and tissues. Although IMS is conceptually simple, sample preparation steps, mass analyzers, and software suites are just a few of the factors that contribute to the successful design of a neuropeptide IMS experiment. This review provides a brief overview of IMS sampling protocols, instrumentation, data analysis tools, technological advancements and applications to neuropeptide localization in neurons and endocrine tissues. Future perspectives in this field are also provided, concluding that neuropeptide IMS would greatly facilitate studies of neuronal network and biomarker discovery.
authors.affiliation: School of Pharmacy, University of Wisconsin-Madison, 777 Highland Avenue, Madison, WI 53705-2222, USA.
authors.forename: Hui
authors.initials: H
authors.lastname: Ye
authors.affiliation: Department of Chemistry, University of Wisconsin-Madison, 777 Highland Avenue, Madison, WI 53705-2222, USA.
authors.forename: Tyler
authors.initials: T
authors.lastname: Greer
authors.affiliation: School of Pharmacy, University of Wisconsin-Madison, 777 Highland Avenue, Madison, WI 53705-2222, USA; Department of Chemistry, University of Wisconsin-Madison, 777 Highland Avenue, Madison, WI 53705-2222, USA. Electronic address: lli@pharmacy.wisc.edu.
authors.forename: Lingjun
authors.initials: L
authors.lastname: Li
mesh_terms: D000825:Animal Structures / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D002451:Cell Compartmentation / Q000502:physiology; D003952:Diagnostic Imaging / Q000379:methods; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D008954:Models, Biological; D015335:Molecular Probes; D009479:Neuropeptides / Q000032:analysis* / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D013048:Specimen Handling / Q000379:methods; D014018:Tissue Distribution
chemical_list: D015335:Molecular Probes; D009479:Neuropeptides