title: Expression of a murine homolog of apoptosis-inducing human IL-24/MDA-7 in murine tumors fails to induce apoptosis or produce anti-tumor effects.
abstract: Expression of human interleukin (IL)-24 in tumors achieved anti-tumor effects through apoptosis. IL-24 also induced secretion of proinflammatory cytokines, suggesting the role in immunity. We showed that murine IL-24 transcripts started from the second initiation codon and that expressed mIL-24 in tumors failed to induce apoptosis. Proliferation of murine cells expressing mIL-24 was the same as that of the parent cells and inoculation of the mIL-24-expressing tumors into syngeneic mice did not produce anti-tumor effects. Secretory mIL-24 did not induce the expression of the IL-6, TNF-α or IFN-γ gene in spleen cells. Expression of mIL-24 receptor subunits, IL-22R and IL-20R1, was undetectable in spleen cells even though they were stimulated by anti-CD3, anti-CD40 antibody or concanavalin A. Transduction of murine tumors with adenoviruses expressing the human IL-24 gene however suppressed the viability and decreased the tumor growth. These data suggest that mIL-24 is functionally irrelevant to the human counterpart.
authors.affiliation: Division of Pathology and Cell Therapy, Chiba Cancer Center Research Institute, 666-2 Nitona, Chuo-ku, Chiba 260-8717, Japan.
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Nagakawa
authors.forename: Osamu
authors.initials: O
authors.lastname: Shimozato
authors.forename: Ling
authors.initials: L
authors.lastname: Yu
authors.forename: Akihiko
authors.initials: A
authors.lastname: Wada
authors.forename: Kiyoko
authors.initials: K
authors.lastname: Kawamura
authors.forename: Quanhai
authors.initials: Q
authors.lastname: Li
authors.forename: Sunil
authors.initials: S
authors.lastname: Chada
authors.forename: Yuji
authors.initials: Y
authors.lastname: Tada
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Takiguchi
authors.forename: Koichiro
authors.initials: K
authors.lastname: Tatsumi
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Tagawa
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis; D002460:Cell Line; D016207:Cytokines / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007378:Interleukins / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D016415:Sequence Alignment; D000099180:Interleukin-24
chemical_list: D016207:Cytokines; D007378:Interleukins; D000099180:Interleukin-24; C431904:Il24 protein, mouse