title: The effect of the hyperbaric environment on heat shock protein 72 expression in vivo.
abstract: Heat shock protein 72 (HSP72) is expressed in response to stress and has been demonstrated to follow a diurnal expression pattern within monocytes and is sensitive to changes in core temperature. Numerous studies have shown changes in HSP72 expression within cell lines exposed to hyperbaric conditions. No studies have investigated changes in HSP72 expression in vivo. Six males participated in the study and were exposed to hyperbaric air and hyperbaric oxygen a week apart. Monocyte HSP72 was analyzed by flow cytometry at 09:00, 13:00, 17:00, 21:00 with hyperbaric oxygen or hyperbaric air breathing commencing at 15:00 for 78 min at a pressure of 2.8 ATA. HSP72 under normoxia followed the established trend; however, following the hyperbaric air or oxygen exposure a reduction in detectable HSP72 was observed at 17:00 and 21:00. No changes in core temperature were observed between 13:00 and 21:00 for any condition. The data show that HSP72 expression is impaired following hyperbaric air (HA) exposure, when compared with control or hyperbaric oxygen (HO) exposure.
authors.affiliation: Muscle Cellular and Molecular Physiology-MCMP & Applied Sport and Exercise Science-ASEP Research Groups, Institute of Sport and Physical Activity Research-ISPAR, Department of Sport and Exercise Sciences, University of Bedfordshire, Bedford, United Kingdom. lee.taylor@beds.ac.uk
authors.forename: Lee
authors.initials: L
authors.lastname: Taylor
authors.forename: Adrian W
authors.initials: AW
authors.lastname: Midgley
authors.forename: Marie E
authors.initials: ME
authors.lastname: Sandstrom
authors.forename: Bryna
authors.initials: B
authors.lastname: Chrismas
authors.forename: Lars R
authors.initials: LR
authors.lastname: McNaughton
mesh_terms: D000222:Adaptation, Physiological; D001831:Body Temperature; D004777:Environment; D005434:Flow Cytometry; D018840:HSP70 Heat-Shock Proteins; D050884:HSP72 Heat-Shock Proteins / Q000096:biosynthesis* / Q000378:metabolism; D006801:Humans; D006931:Hyperbaric Oxygenation / Q000009:adverse effects*; D008297:Male; D009000:Monocytes; D018384:Oxidative Stress; D013312:Stress, Physiological; D013686:Telemetry / Q000295:instrumentation; D013858:Thiobarbiturates / Q000097:blood; D055815:Young Adult
chemical_list: D018840:HSP70 Heat-Shock Proteins; D050884:HSP72 Heat-Shock Proteins; D013858:Thiobarbiturates; C029684:thiobarbituric acid