title: CUE domain-containing protein Vps901 is required for vacuolar protein transport in Schizosaccharomyces pombe.
abstract: The functions of two Schizosaccharomyces pombe Vps9-like genes, SPBC4F6.10/vps901(+) and SPBC29A10.11c/vps902(+), were characterized. Genomic sequence analysis predicted that Vps901p contains a VPS9 domain, whereas cDNA analyses revealed that Vps901p contains a CUE domain (coupling of ubiquitin to ER degradation) in its C-terminal region. Deletion of vps901(+) resulted in mis-sorting and secretion of S. pombe vacuolar carboxypeptidase Cpy1p, whereas deletion of vps902(+) had no effect, suggesting that only Vps901p functions in vacuolar protein transport in S. pombe. Deletion of vps901(+) further produced pleiotropic phenotypes, including vacuolar homotypic fusion and endocytosis defects. Heterologous expression of the budding yeast VPS9 gene corrected the CPY mis-sorting defect in vps901Δ cells. These findings suggest that the VPS9 domain of Vps901p is required for vacuolar protein trafficking in S. pombe.
authors.affiliation: Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Hakozaki, Fukuoka, Japan.
authors.forename: Mai
authors.initials: M
authors.lastname: Nakase
authors.forename: Yuta
authors.initials: Y
authors.lastname: Tsukamoto
authors.forename: Akira
authors.initials: A
authors.lastname: Hosomi
authors.forename: Tomo
authors.initials: T
authors.lastname: Matsuda
authors.forename: Masaaki
authors.initials: M
authors.lastname: Miyamoto
authors.forename: Kaoru
authors.initials: K
authors.lastname: Takegawa
mesh_terms: D000595:Amino Acid Sequence; D002268:Carboxypeptidases / Q000378:metabolism; D004705:Endocytosis; D005816:Genetic Complementation Test; D020662:Guanine Nucleotide Exchange Factors / Q000235:genetics* / Q000378:metabolism; D008561:Membrane Fusion; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary; D021381:Protein Transport / Q000502:physiology*; D012568:Schizosaccharomyces / Q000235:genetics* / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics* / Q000378:metabolism; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid; D014617:Vacuoles / Q000502:physiology*
chemical_list: D020662:Guanine Nucleotide Exchange Factors; D020033:Protein Isoforms; D029702:Schizosaccharomyces pombe Proteins; C000597860:Vps901 protein, S pombe; D002268:Carboxypeptidases