title: Selective chemical imaging of static actin in live cells.
abstract: We have characterized rationally designed and optimized analogues of the actin-stabilizing natural products jasplakinolide and chondramide C. Efficient actin staining was achieved in fixed permeabilized and non-permeabilized cells using different combinations of dye and linker length, thus highlighting the degree of molecular flexibility of the natural product scaffold. Investigations into synthetically accessible, non-toxic analogues have led to the characterization of a powerful cell-permeable probe to selectively image static, long-lived actin filaments against dynamic F-actin and monomeric G-actin populations in live cells, with negligible disruption of rapid actin dynamics.
authors.affiliation: Department of Chemical Biology, Max-Planck-Institute of Molecular Physiology, Dortmund, Germany.
authors.forename: Lech-Gustav
authors.initials: LG
authors.lastname: Milroy
authors.forename: Stefano
authors.initials: S
authors.lastname: Rizzo
authors.forename: Abram
authors.initials: A
authors.lastname: Calderon
authors.forename: Bernhard
authors.initials: B
authors.lastname: Ellinger
authors.forename: Silke
authors.initials: S
authors.lastname: Erdmann
authors.forename: Justine
authors.initials: J
authors.lastname: Mondry
authors.forename: Peter
authors.initials: P
authors.lastname: Verveer
authors.forename: Philippe
authors.initials: P
authors.lastname: Bastiaens
authors.forename: Herbert
authors.initials: H
authors.lastname: Waldmann
authors.forename: Leif
authors.initials: L
authors.lastname: Dehmelt
authors.forename: Hans-Dieter
authors.initials: HD
authors.lastname: Arndt
mesh_terms: D000199:Actins / Q000032:analysis / Q000648:ultrastructure*; D000545:Amanita / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry*; D001688:Biological Products / Q000737:chemistry*; D002460:Cell Line; D002470:Cell Survival; D047630:Depsipeptides / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D008856:Microscopy, Fluorescence; D013194:Staining and Labeling / Q000379:methods
chemical_list: D000199:Actins; D001426:Bacterial Proteins; D001688:Biological Products; D047630:Depsipeptides; D005456:Fluorescent Dyes; C097821:chondramide C; C057531:jasplakinolide