title: Comparative proteomic analysis of early salt stress-responsive proteins in roots of SnRK2 transgenic rice.
abstract: BACKGROUND
  The rice roots are highly salt-sensitive organ and primary root growth is rapidly suppressed by salt stress. Sucrose nonfermenting 1-related protein kinase2 (SnRK2) family is one of the key regulator of hyper-osmotic stress signalling in various plant cells. To understand early salt response of rice roots and identify SnRK2 signaling components, proteome changes of transgenic rice roots over-expressing OSRK1, a rice SnRK2 kinase were investigated.
  
  
  RESULTS
  Proteomes were analyzed by two-dimensional electrophoresis and protein spots were identified by LC-MS/MS from wild type and OSRK1 transgenic rice roots exposed to 150 mM NaCl for either 3 h or 7 h. Fifty two early salt -responsive protein spots were identified from wild type rice roots. The major up-regulated proteins were enzymes related to energy regulation, amino acid metabolism, methylglyoxal detoxification, redox regulation and protein turnover. It is noted that enzymes known to be involved in GA-induced root growth such as fructose bisphosphate aldolase and methylmalonate semialdehyde dehydrogenase were clearly down-regulated. In contrast to wild type rice roots, only a few proteins were changed by salt stress in OSRK1 transgenic rice roots. A comparative quantitative analysis of the proteome level indicated that forty three early salt-responsive proteins were magnified in transgenic rice roots at unstressed condition. These proteins contain single or multiple potential SnRK2 recognition motives. In vitro kinase assay revealed that one of the identified proteome, calreticulin is a good substrate of OSRK1.
  
  
  CONCLUSIONS
  Our present data implicate that rice roots rapidly changed broad spectrum of energy metabolism upon challenging salt stress, and suppression of GA signaling by salt stress may be responsible for the rapid arrest of root growth and development. The broad spectrum of functional categories of proteins affected by over-expression of OSRK1 indicates that OSRK1 is an upstream regulator of stress signaling in rice roots. Enzymes involved in glycolysis, branched amino acid catabolism, dnaK-type molecular chaperone, calcium binding protein, Sal T and glyoxalase are potential targets of OSRK1 in rice roots under salt stress that need to be further investigated.
authors.affiliation: Bio-Crops Development Division, National Academy of Agricultural Sciences, Suwon 441-857, Republic of Korea. isyoon@korea.kr.
authors.forename: Myung Hee
authors.initials: MH
authors.lastname: Nam
authors.forename: Sun Mi
authors.initials: SM
authors.lastname: Huh
authors.forename: Kyung Mi
authors.initials: KM
authors.lastname: Kim
authors.forename: Woong June
authors.initials: WJ
authors.lastname: Park
authors.forename: Jong Bok
authors.initials: JB
authors.lastname: Seo
authors.forename: Kun
authors.initials: K
authors.lastname: Cho
authors.forename: Dool Yi
authors.initials: DY
authors.lastname: Kim
authors.forename: Beom Gi
authors.initials: BG
authors.lastname: Kim
authors.forename: In Sun
authors.initials: IS
authors.lastname: Yoon