title: Structural evidence of perfluorooctane sulfonate transport by human serum albumin.
abstract: Perfluorooctane sulfonate (PFOS) is a man-made fluorosurfactant and globally persistent organic pollutant. PFOS is mainly distributed in blood with a long half-life for elimination. PFOS was found mainly bound to human serum albumin (HSA) in plasma, the most abundant protein in human blood plasma, which transports a variety of endogenous and exogenous ligands. However, the structural basis of such binding remains unclear. Here, we report the crystal structure of the HSA-PFOS complex and show that PFOS binds to HSA at a molar ratio of 2:1. In addition, PFOS binding renders the HSA structure more compact. Our results provide a structural mechanism to understand the retention of surfactants in human serum.
authors.affiliation: State Key laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, China.
authors.forename: Zhipu
authors.initials: Z
authors.lastname: Luo
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Shi
authors.forename: Qin
authors.initials: Q
authors.lastname: Hu
authors.forename: Bin
authors.initials: B
authors.lastname: Zhao
authors.forename: Mingdong
authors.initials: M
authors.lastname: Huang
mesh_terms: D017738:Alkanesulfonic Acids / Q000737:chemistry / Q000378:metabolism*; D001665:Binding Sites; D018360:Crystallography, X-Ray; D005466:Fluorocarbons / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008958:Models, Molecular; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism*
chemical_list: D017738:Alkanesulfonic Acids; D005466:Fluorocarbons; D012709:Serum Albumin; C076994:perfluorooctane sulfonic acid