title: [Changes and the clinical significance of CD4⁺ CD25⁺ regulatory T cells and Th17 cells in peripheral blood of infants with respiratory syncytial virus bronchiolitis].
abstract: OBJECTIVE
  To observe the percentages of CD4(+);CD25(+); regulatory T cells (Tregs) and Th17 cells and the levels of IL-10, TGF-β and IL-17 in peripheral blood of infants with respiratory syncytial virus (RSV) bronchiolitis. The relationship between above cells, cytokines and RSV bronchiolitis was determined.
  
  
  METHODS
  Thirty-three infants with RSV bronchiolitis, twenty-eight infants with non-RSV pneumonia and twenty-six healthy infants were enrolled. The percentages of Tregs and Th17 cells in peripheral blood were detected by flow cytometer (FCM), and the levels of IL-10, TGF-β and IL-17 in plasma were determined by ELISA.
  
  
  RESULTS
  The percentage of Tregs and the levels of IL-10 and TGF-β in infants with RSV bronchiolitis were significantly lower than those in infants with non-RSV pneumonia and healthy infants (P<0.05), while the percentage of Th17 cells and the level of IL-17 in infants with RSV bronchiolitis were significantly higher than those in infants with non-RSV pneumonia and healthy infants (P<0.05).
  
  
  CONCLUSIONS
  The imbalance between Tregs and Th17 cells in peripheral blood of infants with RSV bronchiolitis may be one of the pathogenesis of RSV bronchiolitis.
authors.affiliation: Department of Pediatrics, Binzhou Medical University, Binzhou 256603, China. libinby2009@163.com
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.forename: Fu-Ling
authors.initials: FL
authors.lastname: Wu
authors.forename: Xue-Bin
authors.initials: XB
authors.lastname: Feng
authors.forename: Da-Kang
authors.initials: DK
authors.lastname: Sun
authors.forename: Qing-Qing
authors.initials: QQ
authors.lastname: Cui
authors.forename: Zhi-Xu
authors.initials: ZX
authors.lastname: Zhao
mesh_terms: D001990:Bronchiolitis, Viral / Q000097:blood / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007223:Infant; D016753:Interleukin-10 / Q000097:blood / Q000276:immunology; D020381:Interleukin-17 / Q000097:blood / Q000276:immunology; D053645:Interleukin-2 Receptor alpha Subunit / Q000097:blood / Q000276:immunology; D008297:Male; D018357:Respiratory Syncytial Virus Infections / Q000097:blood / Q000276:immunology*; D012136:Respiratory Syncytial Viruses / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000378:metabolism; D058504:Th17 Cells / Q000276:immunology* / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000097:blood / Q000276:immunology
chemical_list: D020381:Interleukin-17; D053645:Interleukin-2 Receptor alpha Subunit; D016212:Transforming Growth Factor beta; D016753:Interleukin-10