title: Study of mycotoxin calibration approaches on the example of trichothecenes analysis from flour.
abstract: The aim of this study was to evaluate the applicability of different calibration approaches in trichothecenes analysis from wheat flour. Concretely eight trichothecenes (five type B trichothecenes and three type A trichothecenes) were analyzed by matrix solid-phase dispersion (MSPD) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). In the first set of experiments the presence of matrix effects was evaluated; values ranged between 59% and 79%. In the second set of experiments, solutions to compensate these signal suppressions were examined. Different calibration methods showed to tackle matrix effects obtaining values between 69% and 85% for external matrix matched calibration and values from 76% to 111% and from 78% to 108% by analog and deuterated internal standard calibrations, respectively. Trueness of the method was studied using quality control material and certificated reference material (DON 1062 ± 110 μg kg(-1)). The values were compared by matrix-matched, analog internal standard (deepoxydeoxynivalenol (DOM-1)) and deuterated internal standard (DON-d(1)).
authors.affiliation: Departament de Medicina Preventiva i Salut Pública, Facultat de Farmàcia, Universitat de València, Av. Vicent Andrés Estellés, 46100 Burjassot, Spain. josep.rubert@uv.es
authors.forename: Josep
authors.initials: J
authors.lastname: Rubert
authors.forename: Kevin J
authors.initials: KJ
authors.lastname: James
authors.forename: Jordi
authors.initials: J
authors.lastname: Mañes
authors.forename: Carla
authors.initials: C
authors.lastname: Soler
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D003903:Deuterium; D005433:Flour / Q000032:analysis*; D007202:Indicators and Reagents; D009183:Mycotoxins / Q000032:analysis*; D012015:Reference Standards; D053719:Tandem Mass Spectrometry; D014255:Trichothecenes / Q000032:analysis*; D014908:Triticum
chemical_list: D007202:Indicators and Reagents; D009183:Mycotoxins; D014255:Trichothecenes; D003903:Deuterium