title: Electrospun elastin-like polypeptide enriched polyurethanes and their interactions with vascular smooth muscle cells.
abstract: In vascular tissue, elastin is an essential extracellular matrix protein that plays an important biomechanical and biological signalling role. Native elastin is insoluble and is difficult to extract from tissues, which results in its relatively rare use for the fabrication of vascular tissue engineering scaffolds. Recombinant elastin-like polypeptide-4 (ELP4), which mimics the structure and function of native tropoelastin, represents a practical alternative to the native elastic fibre for vascular applications. In this study, electrospinning was utilized to fabricate fibrous scaffolds which were subsequently surface modified with ELP4 and used as substrates for smooth muscle cell culture. ELP4 surface modified materials demonstrated enhanced smooth muscle cell (SMC) adhesion and maintenance of cell numbers over a 1-week period relative to controls. SMCs seeded on the ELP4 surface modified materials were also shown to exhibit the cell morphology and biological markers of a contractile phenotype including a spindle-like morphology, actin filament organization and smooth muscle myosin heavy chain expression. Competitive inhibition experiments demonstrated that the elastin-laminin cell surface receptor and its affinity for the VGVAPG peptide sequence on ELP4 molecules are likely involved in the initial SMC contact with the ELP4 modified materials. Elastin-like polypeptides show promise as surface modifiers for candidate scaffolds for engineering contractile vascular tissues.
authors.affiliation: Department of Chemical Engineering and Applied Chemistry, University of Toronto, Ontario, Canada.
authors.forename: Patrick H
authors.initials: PH
authors.lastname: Blit
authors.forename: Kyle G
authors.initials: KG
authors.lastname: Battiston
authors.forename: Meilin
authors.initials: M
authors.lastname: Yang
authors.forename: J
authors.initials: J
authors.lastname: Paul Santerre
authors.forename: Kimberly A
authors.initials: KA
authors.lastname: Woodhouse
mesh_terms: D008841:Actin Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D000199:Actins / Q000378:metabolism; D000595:Amino Acid Sequence; D015415:Biomarkers / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D002452:Cell Count; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004549:Elastin / Q000494:pharmacology*; D006801:Humans; D007785:Lactose / Q000494:pharmacology; D008422:Materials Testing / Q000379:methods*; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D032389:Myocytes, Smooth Muscle / Q000166:cytology* / Q000187:drug effects* / Q000648:ultrastructure; D018995:Myosin Heavy Chains / Q000378:metabolism; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology; D010641:Phenotype; D011140:Polyurethanes / Q000494:pharmacology*; D013499:Surface Properties / Q000187:drug effects; D013997:Time Factors
chemical_list: D000199:Actins; D015415:Biomarkers; D010455:Peptides; D011140:Polyurethanes; D004549:Elastin; D018995:Myosin Heavy Chains; D007785:Lactose