title: Dental pulp stem cells express proteins involved in the local invasiveness of odontogenic myxoma.
abstract: Little is known about the histogenesis of the odontogenic myxoma (OM). Dental pulp stem cells could be candidate precursors of OM because both OM and the dental pulp share the same embryological origin: the dental papilla. For the purpose of comparing OM and stem cells, this study analyzed the expression of two proteins related to OM invasiveness (MMP-2 and hyaluronic acid) in human immature dental pulp stem cells (hIDPSCs). Three lineages of hIDPSCs from deciduous and permanent teeth were used in this study. Immunofluorescence revealed positive reactions for MMP-2 and hyaluronic acid (HA) in all hIDPSCs. MMP-2 appeared as dots throughout the cytoplasm, whereas HA appeared either as diffuse and irregular dots or as short fibrils throughout the cytoplasm and outside the cell bodies. The gene expression profile of each cell lineage was evaluated using RT-PCR analysis, and HA was expressed more intensively than MMP-2. HA expression was similar among the three hIDPSCs lineages, whereas MMP-2 expression was higher in DL-1 than in the other cell lines. The expression of proteins related to OM invasiveness in hIDPSCs could indicate that OM originates from dental pulp stem cells.
authors.affiliation: Universidade de São Paulo, São Paulo, SP, Brazil. mmmarques@usp.br
authors.forename: Sueli Patricia Harumi
authors.initials: SP
authors.lastname: Miyagi
authors.forename: Carlos Magno da Costa
authors.initials: CM
authors.lastname: Maranduba
authors.forename: Fernando de Sá
authors.initials: Fde S
authors.lastname: Silva
authors.forename: Márcia Martins
authors.initials: MM
authors.lastname: Marques
mesh_terms: D000293:Adolescent; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D003782:Dental Pulp / Q000166:cytology / Q000378:metabolism*; D005109:Extracellular Matrix; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D006801:Humans; D006820:Hyaluronic Acid / Q000235:genetics / Q000378:metabolism*; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism*; D009232:Myxoma / Q000235:genetics / Q000473:pathology*; D009361:Neoplasm Invasiveness / Q000473:pathology; D009808:Odontogenic Tumors / Q000235:genetics / Q000473:pathology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000378:metabolism*
chemical_list: D006820:Hyaluronic Acid; D020778:Matrix Metalloproteinase 2