title: Derivation of iPSCs in stirred suspension bioreactors.
abstract: Induced pluripotent stem cells (iPSCs) are typically derived in adherent culture. Here we report fast and efficient derivation of mouse iPSCs in stirred suspension bioreactors, with and without the use of c-Myc. Suspension-reprogrammed cells expressed pluripotency markers, showed multilineage differentiation in vitro and in vivo, and contributed to the germline in chimeric mice. Suspension reprogramming has the potential to accelerate and standardize iPSC research.
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Shafa
authors.forename: Brad
authors.initials: B
authors.lastname: Day
authors.forename: Akihiro
authors.initials: A
authors.lastname: Yamashita
authors.forename: Guoliang
authors.initials: G
authors.lastname: Meng
authors.forename: Shiying
authors.initials: S
authors.lastname: Liu
authors.forename: Roman
authors.initials: R
authors.lastname: Krawetz
authors.forename: Derrick E
authors.initials: DE
authors.lastname: Rancourt
mesh_terms: D000818:Animals; D019149:Bioreactors; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D002454:Cell Differentiation / Q000502:physiology; D002678:Chimera / Q000235:genetics; D005434:Flow Cytometry; D016130:Immunophenotyping / Q000379:methods; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism; D014162:Transfection / Q000379:methods
chemical_list: C489428:Myc protein, mouse; D016271:Proto-Oncogene Proteins c-myc