title: Decreased soluble TGF-β1, Tie-2, and angiopoietins serum levels in bone marrow after treating healthy donors with granulocyte colony-stimulating factor.
abstract: The goal of this study was to investigate the effect of granulocyte colony-stimulating factor (G-CSF) on Tie-2, angiopoietins, VEGF, and TGF-β1 in bone marrow (BM) of healthy donors. Soluble Tie-2, angiopoietins, VEGF, and TGF-β1 levels in the BM were determined via ELISA in 25 healthy donors before and after G-CSF treatment. The results showed that treating healthy donors with G-CSF significantly decrease serum levels of Tie-2, angiopoietin-1, angiopoietin-2, and TGF-β1. In contrast, median VEGF level in the G-CSF-primed BM was significantly higher than steady-state BM. Our results suggest that decreased soluble TGF-β1, Tie-2, and angiopoietins levels in the BM could be related to stem cell mobilization.
authors.affiliation: Department of Pathology, Third Affiliated Hospital of Hebei Medical University, No. 139, Ziqiang Road, Shijiazhuang 050051, China.
authors.forename: Jian-Zhu
authors.initials: JZ
authors.lastname: Yang
authors.forename: Li-Xia
authors.initials: LX
authors.lastname: Sun
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D042682:Angiopoietins / Q000097:blood*; D001853:Bone Marrow / Q000378:metabolism*; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage*; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods; D006801:Humans; D019520:Living Donors; D008297:Male; D008875:Middle Aged; D020794:Receptor Protein-Tyrosine Kinases / Q000097:blood*; D042787:Receptor, TIE-2; D053773:Transforming Growth Factor beta1 / Q000097:blood*
chemical_list: D042682:Angiopoietins; D053773:Transforming Growth Factor beta1; D016179:Granulocyte Colony-Stimulating Factor; D020794:Receptor Protein-Tyrosine Kinases; D042787:Receptor, TIE-2; C079086:Tek protein, mouse