title: Gene expression and protein levels of thioredoxin in the gills from the whiteleg shrimp (Litopenaeus vannamei) infected with two different viruses: the WSSV or IHHNV.
abstract: The thioredoxin (TRX) system in crustaceans has demonstrated to act as a cell antioxidant being part of the immune response by dealing with the increased production of reactive oxygen species during bacterial or viral infection. Since the number of marine viruses has increased in the last years significantly affecting aquaculture practices of penaeids, and due to the adverse impact on wild and cultured shrimp populations, it is important to elucidate the dynamics of the shrimp response to viral infections. The role of Litopenaeus vannamei thioredoxin (LvTRX) was compared at both, mRNA and protein levels, in response to two viruses, the white spot syndrome virus (WSSV) and the infectious hypodermal and hematopoietic necrosis virus (IHHNV). The results confirmed changes in the TRX gene expression levels of WSSV-infected shrimp, but also demonstrated a more conspicuous response of TRX to WSSV than to IHHNV. While both the dimeric and monomeric forms of LvTRX were detected by Western blot analysis during the WSSV infection, the dimer on its reduced form was only detected through the IHHNV infectious process. These findings indicate that WSSV or IHHNV infected shrimp may induce a differential response of the LvTRX protein.
authors.affiliation: Centro de Investigacion en Alimentacion y Desarrollo (CIAD), Hermosillo, Sonora, Mexico.
authors.forename: Karina D
authors.initials: KD
authors.lastname: Garcia-Orozco
authors.forename: Arturo
authors.initials: A
authors.lastname: Sanchez-Paz
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Aispuro-Hernandez
authors.forename: Silvia
authors.initials: S
authors.lastname: Gomez-Jimenez
authors.forename: Alonso
authors.initials: A
authors.lastname: Lopez-Zavala
authors.forename: Sandra
authors.initials: S
authors.lastname: Araujo-Bernal
authors.forename: Adriana
authors.initials: A
authors.lastname: Muhlia-Almazan
mesh_terms: D000818:Animals; D019223:Densovirinae / Q000502:physiology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D005880:Gills / Q000276:immunology / Q000821:virology; D033561:Penaeidae / Q000276:immunology / Q000821:virology; D013879:Thioredoxins / Q000235:genetics* / Q000276:immunology*; D013997:Time Factors; D046848:White spot syndrome virus 1 / Q000502:physiology*
chemical_list: D013879:Thioredoxins