title: Injection of embryo culture supernatant to the endometrial cavity does not affect outcomes in IVF/ICSI or oocyte donation cycles: a randomized clinical trial.
abstract: OBJECTIVE
  To evaluate whether intrauterine injection of embryo culture supernatant before embryo transfer has any impact on pregnancy and implantation rates.
  
  
  METHODS
  A total of 400 cycles, of which 200 IVF/ICSI and 200 oocyte donor (OD), were randomly assigned to have their uterine cavity injected (group I) or not (group II). Primary endpoints to be studied were pregnancy and implantation rates.
  
  
  RESULTS
  Clinical pregnancy rate per transfer (47.87%, 90/188 versus 48.45%, 94/194) based on transvaginal scan findings at 7 weeks of gestation and implantation rate (25.6% versus 26.5%) were similar in the two groups. The day of embryo transfer, day 3 or day 5, did not affect the final outcome.
  
  
  CONCLUSIONS
  Injection of embryo culture supernatant into the uterine cavity, 30 min before the embryo transfer on either day 3 or 5, neither improves nor adversely affects the pregnancy rate in IVF/ICSI or oocyte donation cycles.
authors.affiliation: Iakentro IVF Center, Agiou Vasileiou 4, 54250 Thessaloniki, Greece.
authors.forename: Yannis
authors.initials: Y
authors.lastname: Prapas
authors.forename: Stamatios
authors.initials: S
authors.lastname: Petousis
authors.forename: Yannis
authors.initials: Y
authors.lastname: Panagiotidis
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Gullo
authors.forename: Lia
authors.initials: L
authors.lastname: Kasapi
authors.forename: Achilleas
authors.initials: A
authors.lastname: Papadeothodorou
authors.forename: Nikos
authors.initials: N
authors.lastname: Prapas
mesh_terms: D000328:Adult; D017077:Culture Media, Conditioned / Q000494:pharmacology; D046149:Embryo Culture Techniques; D010064:Embryo Implantation / Q000187:drug effects*; D004624:Embryo Transfer / Q000379:methods*; D005260:Female; D006801:Humans; D018587:Oocyte Donation; D011247:Pregnancy; D018873:Pregnancy Rate; D011446:Prospective Studies; D020554:Sperm Injections, Intracytoplasmic
chemical_list: D017077:Culture Media, Conditioned