title: Cytogenetic biomonitoring of peripheral blood and oral mucosa cells from car painters.
abstract: The aim of the present study was to comparatively evaluate genomic damage and cellular death in exfoliated oral mucosa cells and peripheral blood from car painters. A total of 24 car painters and 19 healthy controls (non-exposed individuals) were included in this setting. Individuals had epithelial cells from cheek mucosa (left and right side) mechanically exfoliated, placed in fixative and dropped in clean slides which were checked for the specific nuclear phenotypes. A total of 5 μL from peripheral blood was collected for the single cell gel (comet) assay. The results pointed out statistically significant differences (p < 0.05) of micronucleated oral mucosa cells from car painters. In addition, DNA damage was detected in peripheral blood cells by single cell gel (comet) assay. Nevertheless, exposure to car paints did not cause increases other nuclear alterations closely related to cytotoxicity such as karrhyorexis, pyknosis and karyolysis in buccal mucosa cells. In summary, the results of the present study suggest that car painters comprise a high risk group since paints can induce genotoxic and mutagenic effects in peripheral blood and oral mucosa cells, respectively.
authors.affiliation: Department of Biosciences, Federal University of São Paulo, UNIFESP, Santos, SP, Brazil.
authors.forename: Victor Hugo
authors.initials: VH
authors.lastname: Pereira da Silva
authors.forename: Carolina Foot
authors.initials: CF
authors.lastname: Gomes de Moura
authors.forename: Regina Célia
authors.initials: RC
authors.lastname: Spadari-Bratfisch
authors.forename: Daniel
authors.initials: D
authors.lastname: Araki Ribeiro
mesh_terms: D000293:Adolescent; D000328:Adult; D001336:Automobiles; D001773:Blood Cells / Q000187:drug effects* / Q000473:pathology; D016923:Cell Death / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects / Q000473:pathology; D020552:Comet Assay; D004249:DNA Damage; D004784:Environmental Monitoring / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced / Q000706:statistics & numerical data; D015162:Micronucleus Tests; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D009061:Mouth Mucosa / Q000187:drug effects* / Q000473:pathology; D016273:Occupational Exposure / Q000009:adverse effects*; D010150:Paint / Q000633:toxicity*; D055815:Young Adult