title: Staining membrane-bound proteins with ponceau s.
abstract: INTRODUCTIONBecause Ponceau S is relatively insensitive (~1 μg of protein), only the most abundant proteins will be visible. However, it is a reversible stain that can be removed completely with H(2)O prior to processing the blots. After staining, a soft lead pencil can be used to record the presence of visible proteins and molecular-weight markers, which will help when aligning the proteins detected on the membrane by western analysis with those in a total protein-stained gel or membrane. Ponceau S is compatible with both nitrocellulose and PVDF membranes.
authors.forename: Wayne R
authors.initials: WR
authors.lastname: Stochaj
authors.forename: Tom
authors.initials: T
authors.lastname: Berkelman
authors.forename: Nancy
authors.initials: N
authors.lastname: Laird