title: RP-HPLC method for the simultaneous quantitation of boeravinone E and boeravinone B in Boerhaavia diffusa extract and its formulation.
abstract: A high-performance liquid chromatography method was developed and validated for the simultaneous quantitation of two major rotenoids, boeravinone E and boeravinone B, in Boerhaavia diffusa extract and its formulation. Chromatographic separation was carried out on an Inertsil ODS-3 column by using gradient mobile phase containing 0.1% v/v orthophosphoric acid in water and acetonitrile. The detection was carried out at 276 nm. The method was validated for specificity, precision, accuracy and robustness. The linearity (r(2) = 0.9989 and 0.9991) was found to be in the range of 7.26-35.75 µg mL(-1) and 2.20-11.00 µg mL(-1) for boeravinone E and B, respectively. The percent recovery observed from the extract sample was 95.22-95.83.
authors.affiliation: Tulip Lab Pvt. Ltd., F-20/21, MIDC Ranjangaon, Pune 412220, Maharashtra, India. bshrinivas16@gmail.com
authors.forename: S G
authors.initials: SG
authors.lastname: Bhope
authors.forename: P S
authors.initials: PS
authors.lastname: Gaikwad
authors.forename: V V
authors.initials: VV
authors.lastname: Kuber
authors.forename: M J
authors.initials: MJ
authors.lastname: Patil
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005419:Flavonoids / Q000032:analysis*; D039281:Nyctaginaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry*
chemical_list: D005419:Flavonoids; D010936:Plant Extracts; C560379:boeravinone B; C560378:boeravinone E