title: Analysis of the targeting properties of Fc chimeric proteins and antibodies in mice in vivo.
abstract: This unit includes protocols for the analysis of the targeting properties of Fc chimeric proteins and antibodies in mice in vivo. We present procedures to prepare the reagents to be tested under endotoxin-free conditions, suitable injection sites, methodology for tissue collection, and processing, and labeling procedures to detect the injected material in situ.
authors.affiliation: Faculty of Medicine and Health Sciences, University of Nottingham, Nottingham, United Kingdom.
authors.forename: Luisa
authors.initials: L
authors.lastname: Martinez-Pomares
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000008:administration & dosage* / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D007074:Immunoglobulin G / Q000008:administration & dosage* / Q000032:analysis* / Q000276:immunology; D007150:Immunohistochemistry / Q000379:methods*; D016130:Immunophenotyping; D051379:Mice; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage / Q000032:analysis / Q000276:immunology; D013194:Staining and Labeling
chemical_list: D000911:Antibodies, Monoclonal; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D011993:Recombinant Fusion Proteins