title: Characterization of protease from Alcaligens faecalis and its antibacterial activity on fish pathogens.
abstract: Alcaligens faecalis AU01 isolated from seafood industry effluent produced an alkaline protease. The optimum culture conditions for growth as well as enzyme production were 37 degrees C and pH 8. The partially purified protease had specific activity of 9.66 with 17.77% recovery with the molecular weight of 33 kDa and it was active between 30-70 degrees C and optimum being at 55 degrees C and pH 9. The enzyme retains more than 85% activity at 70 degrees C and 78% even at pH 10. The enzyme inhibited the growth of fish pathogens such as Flavobacterium sp., Pseudomonas fluorescens, Vibrio harveyi, Proteus sp. and Vibrio parahaemolyticus. From the present study it can be concluded that Alcaligens faecalis AU01 has the potential for aquaculture as probiotic agent and other several applications.
authors.affiliation: Faculty of Marine Science, CAS in Marine Biology, Annamalai University, Parangipettai, 608 502, India. annabact@gmail.com
authors.forename: N
authors.initials: N
authors.lastname: Annamalai
authors.forename: Arun
authors.initials: A
authors.lastname: Kumar
authors.forename: A
authors.initials: A
authors.lastname: Saravanakumar
authors.forename: S
authors.initials: S
authors.lastname: Vijaylakshmi
authors.forename: T
authors.initials: T
authors.lastname: Balasubramanian
mesh_terms: D042481:Alcaligenes faecalis / Q000201:enzymology*; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D005393:Fish Diseases / Q000382:microbiology; D005399:Fishes; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D010447:Peptide Hydrolases / Q000378:metabolism* / Q000494:pharmacology*; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D010447:Peptide Hydrolases