title: Voltage-gated sodium channel activity promotes prostate cancer metastasis in vivo.
abstract: Epigenetic upregulation of voltage-gated sodium channels (VGSCs) has been reported in a number of carcinoma cell lines and tissues. Furthermore, a large body of experimental evidence suggested that functional VGSC expression enhances various in vitro cell behaviours, such as directional motility, that would be involved in the metastatic cascade. However, it is not known if VGSC activity promotes metastasis in vivo. Here, using the Copenhagen rat model of prostate cancer and blocking VGSC activity in primary tumours with tetrodotoxin, we show (1) that the number of lung metastasis is reduced by >40% and (2) that lifespan is significantly improved.
authors.affiliation: Department of Biology, Faculty of Science, Istanbul University, Vezneciler, Istanbul 34134, Turkey; Division of Cell & Molecular Biology, Faculty of Natural Sciences, Sir Alexander Fleming Building, Imperial College London, South Kensington Campus, London SW7 2AZ, UK.
authors.forename: Senay
authors.initials: S
authors.lastname: Yildirim
authors.affiliation: Department of Biology, Faculty of Science, Istanbul University, Vezneciler, Istanbul 34134, Turkey.
authors.forename: Seyhan
authors.initials: S
authors.lastname: Altun
authors.affiliation: Department of Biology, Faculty of Science, Istanbul University, Vezneciler, Istanbul 34134, Turkey; Department of Biology, Faculty of Science & Arts, Harran University, Osmanbey Campus, Sanliurfa, Turkey.
authors.forename: Hatice
authors.initials: H
authors.lastname: Gumushan
authors.affiliation: Department of Urology, Faculty of Medicine, Imperial College London, St. Mary's Hospital Campus, Praed Street, London W2 1NY, UK.
authors.forename: Anup
authors.initials: A
authors.lastname: Patel
authors.affiliation: Division of Cell & Molecular Biology, Faculty of Natural Sciences, Sir Alexander Fleming Building, Imperial College London, South Kensington Campus, London SW7 2AZ, UK; Cyprus International University, Biotechnology Research Centre, Haspolat, North Cyprus, Mersin 10, Turkey. Electronic address: m.djamgoz@imperial.ac.uk.
authors.forename: Mustafa B A
authors.initials: MBA
authors.lastname: Djamgoz
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D008297:Male; D062556:NAV1.7 Voltage-Gated Sodium Channel; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology*; D051381:Rats; D026941:Sodium Channel Blockers / Q000494:pharmacology; D015222:Sodium Channels / Q000378:metabolism*; D013779:Tetrodotoxin / Q000494:pharmacology
chemical_list: D062556:NAV1.7 Voltage-Gated Sodium Channel; C503480:Scn9a protein, rat; D026941:Sodium Channel Blockers; D015222:Sodium Channels; D013779:Tetrodotoxin