title: Non-linear microscopy of smooth muscle cells in artificial extracellular matrices made of cellulose.
abstract: Non-linear microscopy has been used to characterize bovine smooth muscle cells and their proliferation, migration, and differentiation in hydrogel cellulose scaffolds, toward the development of fully functional blood vessel implants. The extracellular matrix (ECM) composed of cellulose and endogenous collagen fibers was imaged using Second Harmonic Generation (SHG) microscopy and the cell morphology by Coherent Anti-Stokes Raman Scattering (CARS) microscopy. Images prove that cells adhere on the cellulose scaffold without additional surface modification and that both contractile and proliferating phenotypes are developed. This work shows that non-linear microscopy contributes with unique insights in cell interactions with (artificial) ECM components and has the potential to become an established characterization method in tissue engineering.
authors.affiliation: Molecular Microscopy, Department of Chemical and Biological Engineering, Chalmers University of Technology, Göteborg, Sweden.
authors.forename: Christian
authors.initials: C
authors.lastname: Brackmann
authors.forename: Jan-Olof
authors.initials: JO
authors.lastname: Dahlberg
authors.forename: Nihal Engin
authors.initials: NE
authors.lastname: Vrana
authors.forename: Caitriona
authors.initials: C
authors.lastname: Lally
authors.forename: Paul
authors.initials: P
authors.lastname: Gatenholm
authors.forename: Annika
authors.initials: A
authors.lastname: Enejder
mesh_terms: D000818:Animals; D032701:Biomimetics; D002417:Cattle; D002448:Cell Adhesion; D002454:Cell Differentiation; D002465:Cell Movement; D049109:Cell Proliferation; D002482:Cellulose / Q000378:metabolism*; D003094:Collagen / Q000096:biosynthesis; D005109:Extracellular Matrix / Q000378:metabolism*; D008853:Microscopy / Q000379:methods*; D032389:Myocytes, Smooth Muscle / Q000166:cytology* / Q000378:metabolism; D013059:Spectrum Analysis, Raman
chemical_list: D002482:Cellulose; D003094:Collagen