title: [Expression of RAS protein in glioma and its effect on the growth of human glioma cells].
abstract: OBJECTIVE
  To study the expression of RAS protein in human glioma tissues and its influence on tumor growth.
  
  
  METHODS
  RAS protein expression in glioma tissues was determined by immunohistochemical (IHC) staining. Subsequently, MTT cell proliferation assay, flow cytometry and Western blotting were used to assay U251 cells with reduced RAS expression.
  
  
  RESULTS
  The expression of RAS in glioma was increased and strongly correlated with pathological grade. Downregulation of RAS resulted in glioma cells growth suppression and increased apoptosis.
  
  
  CONCLUSIONS
  The expression level of RAS protein in human glioma was increased. Downregulation of RAS can inhibit glioblastoma cell growth through the RAS signal pathway.
authors.affiliation: Department of Neurosurgery, Nanjing Medical University, Nanjing, Jiangsu, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Cao
authors.forename: Ying-yi
authors.initials: YY
authors.lastname: Wang
authors.forename: Xi-rui
authors.initials: XR
authors.lastname: Wang
authors.forename: Xie-feng
authors.initials: XF
authors.lastname: Wang
authors.forename: Guan
authors.initials: G
authors.lastname: Sun
authors.forename: Hui
authors.initials: H
authors.lastname: Luo
authors.forename: Ning
authors.initials: N
authors.lastname: Liu
authors.forename: Yong-ping
authors.initials: YP
authors.lastname: You
mesh_terms: D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D048708:Cell Growth Processes / Q000235:genetics; D045744:Cell Line, Tumor; D015536:Down-Regulation; D005910:Glioma / Q000235:genetics* / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D018631:ras Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D018631:ras Proteins