title: Staphylococcus aureus manganese transport protein C is a highly conserved cell surface protein that elicits protective immunity against S. aureus and Staphylococcus epidermidis.
abstract: Staphylococcus aureus and other staphylococci cause severe human disease, and there are currently no vaccines available. We evaluated whether manganese transport protein C (MntC), which is conserved across the staphylococcal species group, could confer protection against S. aureus and Staphylococcus epidermidis. In vivo analysis of S. aureus MntC expression revealed that expression occurs very early during the infectious cycle. Active immunization with MntC was effective at reducing the bacterial load associated with S. aureus and S. epidermidis infection in an acute murine bacteremia model. Anti-MntC monoclonal antibodies have been identified that can bind S. aureus and S. epidermidis cells and are protective in an infant rat passive protection model and induce neutrophil respiratory burst activity. This is the first description of a protein that has the potential to provide protection across the staphylococcal species group.
authors.affiliation: Pfizer Vaccine Research, Pearl River, NY, USA. annaliesa.anderson@pfizer.com
authors.forename: Annaliesa S
authors.initials: AS
authors.lastname: Anderson
authors.forename: Ingrid L
authors.initials: IL
authors.lastname: Scully
authors.forename: Yekaterina
authors.initials: Y
authors.lastname: Timofeyeva
authors.forename: Ellen
authors.initials: E
authors.lastname: Murphy
authors.forename: Lisa K
authors.initials: LK
authors.lastname: McNeil
authors.forename: Terri
authors.initials: T
authors.lastname: Mininni
authors.forename: Lorna
authors.initials: L
authors.lastname: Nuñez
authors.forename: Marjolaine
authors.initials: M
authors.lastname: Carriere
authors.forename: Christine
authors.initials: C
authors.lastname: Singer
authors.forename: Deborah A
authors.initials: DA
authors.lastname: Dilts
authors.forename: Kathrin U
authors.initials: KU
authors.lastname: Jansen
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D016470:Bacteremia / Q000276:immunology / Q000517:prevention & control / Q000628:therapy; D058491:Bacterial Load; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D002352:Carrier Proteins / Q000235:genetics / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D007116:Immunization, Passive; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology*; D051379:Mice; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D013203:Staphylococcal Infections / Q000276:immunology / Q000517:prevention & control* / Q000628:therapy; D013209:Staphylococcal Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D013211:Staphylococcus aureus / Q000276:immunology*; D013212:Staphylococcus epidermidis / Q000276:immunology*; D016896:Treatment Outcome
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D002352:Carrier Proteins; D008565:Membrane Proteins; D013209:Staphylococcal Vaccines