title: Surface plasmon resonance shows a gender difference in circulating annexin A5 in human.
abstract: The level of circulating anxA5 is correlated to various diseases such as acute myocardial infarction, trauma, thrombosis, inflammation and in some cancers. Our aim was to assess whether a direct approach using surface plasmon resonance (SPR) could be easily used to provide a rapid and cheap alternative to detect anxA5 in blood samples in human. Our results indicate that SPR permits to detect and quantify circulating anxA5 in serum with a minimum time of manipulation. Furthermore, we report here, for the first time, that the level of circulating anxA5 is significantly higher in male than in female (5.43 (± 0.02) and 4.41 (± 0.2)ng/ml, respectively). In conclusion, we found that SPR can be used to rapidly quantify anxA5 in patients and that a gender difference has to be taken into account to explain gender differences in the prevalence of some diseases.
authors.affiliation: Inserm, UMR1078, Brest F-29218, France. pascal.trouve@univ-brest.fr
authors.forename: Pascal
authors.initials: P
authors.lastname: Trouvé
authors.forename: Mathieu
authors.initials: M
authors.lastname: Kerbiriou
authors.forename: Sophie
authors.initials: S
authors.lastname: le Hir
authors.forename: Nathalie
authors.initials: N
authors.lastname: Benz
authors.forename: Claude
authors.initials: C
authors.lastname: Férec
mesh_terms: D017304:Annexin A5 / Q000097:blood* / Q000276:immunology; D055910:Antibodies, Immobilized / Q000276:immunology; D002138:Calibration; D004632:Emergency Medical Services; D005260:Female; D006801:Humans; D008297:Male; D015203:Reproducibility of Results; D012727:Sex Characteristics; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D017304:Annexin A5; D055910:Antibodies, Immobilized