title: Ascaris suum enolase is a potential vaccine candidate against ascariasis.
abstract: Ascariasis caused by Ascaris is the most common parasite problem in humans and pigs worldwide. No vaccines are available for the prevention of Ascaris infections. In the present study, the gene encoding Ascaris suum enolase (As-enol-1) was amplified, cloned and sequenced. Amino acid sequence alignment indicated that As-enol-1 was highly conserved between different nematodes and shared the highest identity (87%) with enolase from Anisakis simplex s.l. The recombinant pVAX-Enol was successfully expressed in Marc-145 cells. The ability of the pVAX-Enol for inducing immune protective responses against challenge infection with A. suum L3 was evaluated in Kunming mice. The immune response was evaluated by lymphoproliferative assay, cytokine and antibody measurements, and the reduction rate of recovery larvae. The results showed that the mice immunized with pVAX-Enol developed a high level of specific antibody responses against A. suum, a strong lymphoproliferative response, and significant levels of IFN-γ, IL-2, IL-4 and IL-10 production, compared with the other groups immunized with empty plasmid or blank controls, respectively. There was a 61.13% reduction (P<0.05) in larvae recovery compared with that in the blank control group. Our data indicated that A. suum enolase is a potential vaccine candidate against A. suum infection.
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China.
authors.forename: Ning
authors.initials: N
authors.lastname: Chen
authors.forename: Zi-Guo
authors.initials: ZG
authors.lastname: Yuan
authors.forename: Min-Jun
authors.initials: MJ
authors.lastname: Xu
authors.forename: Dong-Hui
authors.initials: DH
authors.lastname: Zhou
authors.forename: Xiu-Xiang
authors.initials: XX
authors.lastname: Zhang
authors.forename: Yan-Zhong
authors.initials: YZ
authors.lastname: Zhang
authors.forename: Xiao-Wei
authors.initials: XW
authors.lastname: Wang
authors.forename: Chao
authors.initials: C
authors.lastname: Yan
authors.forename: Rui-Qing
authors.initials: RQ
authors.lastname: Lin
authors.forename: Xing-Quan
authors.initials: XQ
authors.lastname: Zhu
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D001196:Ascariasis / Q000276:immunology / Q000517:prevention & control*; D017165:Ascaris suum / Q000201:enzymology* / Q000235:genetics / Q000276:immunology*; D049109:Cell Proliferation; D003001:Cloning, Molecular; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D007963:Leukocytes, Mononuclear / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D010751:Phosphopyruvate Hydratase / Q000235:genetics / Q000276:immunology*; D017422:Sequence Analysis, DNA; D014611:Vaccination / Q000379:methods*; D014612:Vaccines / Q000008:administration & dosage* / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D016207:Cytokines; D014612:Vaccines; D010751:Phosphopyruvate Hydratase