title: Development of a novel label-free amperometric immunosensor for the detection of okadaic acid.
abstract: Okadaic acid (OA), a lipophilic phycotoxin is mainly produced by toxigenic dinoflagellates. The need to develop high performing methods for OA analysis able to improve the traditional ones is evident. In this work, a novel experimental methodology for label-free detection of OA was developed. Protein G magnetic beads (protein-G-MBs) modified gold electrode was used to immobilize anti-OA monoclonal antibody (anti-OA-MAb). Preliminary, colorimetric tests were performed in order to validate protein-G-MBs and anti-OA-MAb reaction. Electrochemical detection was carried out by differential pulse voltammetry in ferri/ferrocyanide solution. The limit of detection value obtained (0.5 μg L(-1)) validated the developed electrochemical immunosensor as a promising tool for routine use. The matrix effect and the recovery rate were also assessed with real samples, showing a good percentage of recovery.
authors.affiliation: Université Perpignan Via Domitia, Institut de Modélisation et d'Analyse en Géo-Environnement et Santé, Perpignan, France.
authors.forename: Akhtar
authors.initials: A
authors.lastname: Hayat
authors.forename: Lise
authors.initials: L
authors.lastname: Barthelmebs
authors.forename: Audrey
authors.initials: A
authors.lastname: Sassolas
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Marty
mesh_terms: D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology; D001426:Bacterial Proteins / Q000737:chemistry / Q000276:immunology; D015374:Biosensing Techniques / Q000379:methods*; D049872:Bivalvia / Q000737:chemistry*; D002138:Calibration; D003124:Colorimetry; D004563:Electrochemistry; D004566:Electrodes; D005292:Ferricyanides / Q000737:chemistry; D005295:Ferrocyanides / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D057230:Limit of Detection; D058185:Magnetite Nanoparticles / Q000737:chemistry; D019319:Okadaic Acid / Q000032:analysis*
chemical_list: D055910:Antibodies, Immobilized; D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D005292:Ferricyanides; D005295:Ferrocyanides; C034284:IgG Fc-binding protein, Streptococcus; D058185:Magnetite Nanoparticles; D019319:Okadaic Acid; D006046:Gold; C031835:potassium ferrocyanide; C028033:potassium ferricyanide