title: Cross-linked DNA: propargylated ribonucleosides as "click" ligation sites for bifunctional azides.
abstract: 2'-O or 3'-O-propargylated adenosines and ribothymidines were used as click targets for cross-linking of oligonucleotides with aliphatic and aromatic azides. The cross-link generates a sugar modification at the 2'-O-ligation site. Inexpensive ribonucleosides were used as starting materials. Cross-linking of oligonucleotides was performed at internal or terminal positions. Hybridization of homodimers with two complementary single strands resulted in stable ligated DNA duplexes.
authors.affiliation: Laboratory of Bioorganic Chemistry and Chemical Biology, Center for Nanotechnology, Heisenbergstraße 11, 48149 Münster, Germany.
authors.forename: Suresh S
authors.initials: SS
authors.lastname: Pujari
authors.forename: Frank
authors.initials: F
authors.lastname: Seela
mesh_terms: D001386:Azides / Q000737:chemistry*; D001483:Base Sequence; D003432:Cross-Linking Reagents; D004247:DNA / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry*; D008026:Ligation; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009841:Oligonucleotides / Q000737:chemistry*; D010293:Pargyline / Q000031:analogs & derivatives* / Q000737:chemistry*; D012263:Ribonucleosides / Q000737:chemistry*
chemical_list: D001386:Azides; D003432:Cross-Linking Reagents; D004277:DNA, Single-Stranded; D009841:Oligonucleotides; D012263:Ribonucleosides; D004247:DNA; D010293:Pargyline