title: Narciclasine inhibits the responses of Arabidopsis roots to auxin.
abstract: The plant hormone auxin plays a central role in the regulation of plant growth and development, as well as in responses to environmental stimuli. Narciclasine (NCS, an Amaryllidaceae alkaloid) isolated from Narcissus tazetta bulbs has a broad range of inhibitory effects on plants. In this study, the role of NCS in responses to auxin in Arabidopsis thaliana roots was investigated. We demonstrated the inhibitory effects of NCS on auxin-inducible lateral root formation, root hair formation, primary root growth, and the expression of primary auxin-inducible genes in Arabidopsis roots using DR5::GUS reporter gene, native auxin promoters (IAA12::GUS, IAA13::GUS), and quantitative reverse transcription PCR analysis. Results also showed that NCS did not affect the expression of cytokinin-inducible ARR5::GUS reporter gene. NCS relieved the auxin-enhanced degradation of the Aux/IAA repressor modulated by the SCFTIR1 ubiquitin-proteasome pathway. In addition, NCS did not alter the auxin-stimulated interaction between IAA7/AXR2 (Aux/IAA proteins) and the F-box protein TIR1 activity of the proteasome. Taken together, these results suggest that NCS acts on the auxin signaling pathway upstream of TIR1, which modulates Aux/IAA protein degradation, and thereby affects the auxin-mediated responses in Arabidopsis roots.
authors.affiliation: School of Life Sciences, Lanzhou University, Lanzhou, 730000, People's Republic of China.
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Hu
authors.forename: Lijing
authors.initials: L
authors.lastname: Yang
authors.forename: Xiaofan
authors.initials: X
authors.lastname: Na
authors.forename: Jia
authors.initials: J
authors.lastname: You
authors.forename: Wei
authors.initials: W
authors.lastname: Hu
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Liang
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.forename: Lina
authors.initials: L
authors.lastname: Mao
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Wang
authors.forename: Huahua
authors.initials: H
authors.lastname: Wang
authors.forename: Yurong
authors.initials: Y
authors.lastname: Bi
mesh_terms: D047151:Amaryllidaceae Alkaloids / Q000302:isolation & purification / Q000494:pharmacology*; D017360:Arabidopsis / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017930:Genes, Reporter; D007210:Indoleacetic Acids / Q000378:metabolism*; D010617:Phenanthridines / Q000302:isolation & purification / Q000494:pharmacology*; D010641:Phenotype; D010937:Plant Growth Regulators / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D011401:Promoter Regions, Genetic / Q000235:genetics; D046988:Proteasome Endopeptidase Complex; D059748:Proteolysis; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics
chemical_list: D047151:Amaryllidaceae Alkaloids; D029681:Arabidopsis Proteins; D007210:Indoleacetic Acids; D010617:Phenanthridines; D010937:Plant Growth Regulators; C010753:narciclasine; D046988:Proteasome Endopeptidase Complex