title: Overexpression of a Medicago truncatula stress-associated protein gene (MtSAP1) leads to nitric oxide accumulation and confers osmotic and salt stress tolerance in transgenic tobacco.
abstract: The impact of Medicago truncatula stress-associated protein gene (MtSAP1) overexpression has been investigated in Nicotiana tabacum transgenic seedlings. Under optimal conditions, transgenic lines overexpressing MtSAP1 revealed better plant development and higher chlorophyll content as compared to wild type seedlings. Interestingly, transgenic lines showed a stronger accumulation of nitric oxide (NO), a signaling molecule involved in growth and development processes. This NO production seemed to be partially nitrate reductase dependent. Due to the fact that NO has been also reported to play a role in tolerance acquisition of plants to abiotic stresses, the responses of MtSAP1 overexpressors to osmotic and salt stress have been studied. Compared to the wild type, transgenic lines were less affected in their growth and development. Moreover, NO content in MtSAP1 overexpressors was always higher than that detected in wild seedlings under stress conditions. It seems that this better tolerance induced by MtSAP1 overexpression could be associated with this higher NO production that would enable seedlings to reach a high protection level to prepare them to cope with abiotic stresses.
authors.affiliation: University of Angers, UMR 1345 Research Institute of Horticulture and Seeds (INRA, Agrocampus-Ouest, University of Angers), SFR 4207 Quasav, 2 Bd Lavoisier, 49045, Angers cedex, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Charrier
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Planchet
authors.forename: Delphine
authors.initials: D
authors.lastname: Cerveau
authors.forename: Christine
authors.initials: C
authors.lastname: Gimeno-Gilles
authors.forename: Isabelle
authors.initials: I
authors.lastname: Verdu
authors.forename: Anis M
authors.initials: AM
authors.lastname: Limami
authors.forename: Eric
authors.initials: E
authors.lastname: Lelièvre
mesh_terms: D000222:Adaptation, Physiological / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D046913:Medicago truncatula / Q000235:genetics*; D009569:Nitric Oxide / Q000032:analysis / Q000378:metabolism*; D009995:Osmosis / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics; D055049:Salt Tolerance; D036226:Seedlings / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D012639:Seeds / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D013312:Stress, Physiological / Q000502:physiology*; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000502:physiology*
chemical_list: D010940:Plant Proteins; D018749:RNA, Plant; D009569:Nitric Oxide