title: A dual role of linker histone H1.4 Lys 34 acetylation in transcriptional activation.
abstract: The linker histone H1 is a key player in chromatin organization, yet our understanding of the regulation of H1 functions by post-translational modifications is very limited. We provide here the first functional characterization of H1 acetylation. We show that H1.4K34 acetylation (H1.4K34ac) is mediated by GCN5 and is preferentially enriched at promoters of active genes, where it stimulates transcription by increasing H1 mobility and recruiting a general transcription factor. H1.4K34ac is dynamic during spermatogenesis and marks undifferentiated cells such as induced pluripotent stem (iPS) cells and testicular germ cell tumors. We propose a model for H1.4K34ac as a novel regulator of chromatin function with a dual role in transcriptional activation.
authors.affiliation: Max Planck Institute of Immunobiology and Epigenetics, Freiburg, Germany.
authors.forename: Kinga
authors.initials: K
authors.lastname: Kamieniarz
authors.forename: Annalisa
authors.initials: A
authors.lastname: Izzo
authors.forename: Miroslav
authors.initials: M
authors.lastname: Dundr
authors.forename: Philipp
authors.initials: P
authors.lastname: Tropberger
authors.forename: Luka
authors.initials: L
authors.lastname: Ozretic
authors.forename: Jutta
authors.initials: J
authors.lastname: Kirfel
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Scheer
authors.forename: Philippe
authors.initials: P
authors.lastname: Tropel
authors.forename: Jacek R
authors.initials: JR
authors.lastname: Wisniewski
authors.forename: Laszlo
authors.initials: L
authors.lastname: Tora
authors.forename: Stephane
authors.initials: S
authors.lastname: Viville
authors.forename: Reinhard
authors.initials: R
authors.lastname: Buettner
authors.forename: Robert
authors.initials: R
authors.lastname: Schneider
mesh_terms: D000107:Acetylation; D000595:Amino Acid Sequence; D002453:Cell Cycle / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D051548:Histone Acetyltransferases; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008239:Lysine / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D039904:Pluripotent Stem Cells / Q000378:metabolism; D011401:Promoter Regions, Genetic; D018239:Seminoma / Q000235:genetics / Q000378:metabolism; D013091:Spermatogenesis / Q000235:genetics; D035381:TATA-Binding Protein Associated Factors / Q000378:metabolism; D013736:Testicular Neoplasms / Q000235:genetics / Q000378:metabolism; D035362:Transcription Factor TFIID / Q000378:metabolism; D024363:Transcription Initiation Site; D015533:Transcriptional Activation; D015854:Up-Regulation; D050880:p300-CBP Transcription Factors / Q000378:metabolism*; D000099224:p300-CBP-Associated Factor
chemical_list: D051548:Histone Acetyltransferases; D006657:Histones; D008239:Lysine; D035381:TATA-Binding Protein Associated Factors; D035362:Transcription Factor TFIID; D050880:p300-CBP Transcription Factors; D000099224:p300-CBP-Associated Factor; C069001:TATA-binding protein associated factor 250 kDa