title: Analysis of gene expression levels in individual bacterial cells without image segmentation.
abstract: Studies of stochasticity in gene expression typically make use of fluorescent protein reporters, which permit the measurement of expression levels within individual cells by fluorescence microscopy. Analysis of such microscopy images is almost invariably based on a segmentation algorithm, where the image of a cell or cluster is analyzed mathematically to delineate individual cell boundaries. However segmentation can be ineffective for studying bacterial cells or clusters, especially at lower magnification, where outlines of individual cells are poorly resolved. Here we demonstrate an alternative method for analyzing such images without segmentation. The method employs a comparison between the pixel brightness in phase contrast vs fluorescence microscopy images. By fitting the correlation between phase contrast and fluorescence intensity to a physical model, we obtain well-defined estimates for the different levels of gene expression that are present in the cell or cluster. The method reveals the boundaries of the individual cells, even if the source images lack the resolution to show these boundaries clearly.
authors.affiliation: Physics Department, University of Florida, PO Box 118440, Gainesville, FL 32611-8440, USA.
authors.forename: In Hae
authors.initials: IH
authors.lastname: Kwak
authors.forename: Minjun
authors.initials: M
authors.lastname: Son
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Hagen
mesh_terms: D001419:Bacteria / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008856:Microscopy, Fluorescence; D012984:Software; D013295:Streptococcus mutans / Q000235:genetics
chemical_list: D049452:Green Fluorescent Proteins