title: Chondroitin sulfate and epidermal growth factor immobilization after plasma polymerization: a versatile anti-apoptotic coating to promote healing around stent grafts.
abstract: Bioactive coatings constitute an interesting approach to enhance healing around implants, such as stent-grafts used in endovascular aneurysm repair. Three different plasma techniques, namely NH₃ plasma functionalization and atmospheric- or low-pressure plasma polymerization, are compared to create amino groups and covalently bind CS and EGF bioactive molecules on PET. The latter presents the greatest potential. CS + EGF coating is shown to strongly decrease cell apoptosis and cell depletion in serum-free medium, while increasing cell growth compared to unmodified PET. This versatile biomimetic coating holds promise in promoting vascular repair around stent-grafts, where resistance to apoptosis is a key issue.
authors.affiliation: Research Centre, Centre Hospitalier de l'Université de Montréal-CRCHUM, 1560 Rue Sherbrooke Est, Montréal-Qc H2L 4M1, Canada.
authors.forename: Cindy
authors.initials: C
authors.lastname: Charbonneau
authors.forename: Juan-Carlos
authors.initials: JC
authors.lastname: Ruiz
authors.forename: Pauline
authors.initials: P
authors.lastname: Lequoy
authors.forename: Marie-Josée
authors.initials: MJ
authors.lastname: Hébert
authors.forename: Gregory
authors.initials: G
authors.lastname: De Crescenzo
authors.forename: Michael R
authors.initials: MR
authors.lastname: Wertheimer
authors.forename: Sophie
authors.initials: S
authors.lastname: Lerouge
mesh_terms: D000783:Aneurysm / Q000628:therapy; D017209:Apoptosis / Q000187:drug effects*; D002478:Cells, Cultured; D002809:Chondroitin Sulfates / Q000737:chemistry / Q000494:pharmacology; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry / Q000494:pharmacology; D015607:Stents
chemical_list: D020099:Coated Materials, Biocompatible; D055767:Immobilized Proteins; D004815:Epidermal Growth Factor; D002809:Chondroitin Sulfates