title: Human platelet lysate is an alternative to fetal bovine serum for large-scale expansion of bone marrow-derived mesenchymal stromal cells.
abstract: Human platelet lysate (HPL) was evaluated as an alternative to fetal bovine serum (FBS) in large-scale culturing of bone marrow-derived mesenchymal stromal cells (BM-MSCs) for therapeutic applications. Dulbecco's modified Eagle medium (DMEM)of low glucose (LG) and Knock Out (KO) were used with human platelet lysate (HPL) as LG-HPL and KO-HPL, and with FBS as LG-FBS and KO-FBS to culture the BM-MSCs. HPL at 10 % (v/v) supported BM-MSCs growth and subsequent isolation efficiency generated >90 × 10(6) MSCs in LG-HPL. Population doublings (PDs) and population doubling times of LG-HPL and KO-HPL (PDT) were not significantly different but LG-HPL showed a significant clonogenic potential and HPL cultures had an average PDT of 36.5 ± 6.5 h and an average PDs of 5 ± 0.7/passage. BM-MSCs cultured with LG-HPL had significantly higher immunosuppression compared to LG-FBS, but KO-HPL and KO-FBS-grown cultures were not significantly different. HPL is therefore alternative to FBS for large-scale production of BM-MSCs for therapeutic applications.
authors.affiliation: Stempeutics Research Pvt. Ltd, Shirdi Sai Baba Cancer Hospital, Manipal, India.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Gottipamula
authors.forename: Archana
authors.initials: A
authors.lastname: Sharma
authors.forename: Sagar
authors.initials: S
authors.lastname: Krishnamurthy
authors.forename: Anish Sen
authors.initials: AS
authors.lastname: Majumdar
authors.forename: Raviraja N
authors.initials: RN
authors.lastname: Seetharam
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000737:chemistry*; D001853:Bone Marrow; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods; D002457:Cell Extracts; D003470:Culture Media / Q000737:chemistry*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D044967:Serum / Q000737:chemistry / Q000378:metabolism
chemical_list: D002457:Cell Extracts; D003470:Culture Media