title: Abnormal development at early postimplantation stage in mouse embryos after preimplantation genetic diagnosis.
abstract: Preimplantation genetic diagnosis (PGD) is an established procedure for the genetic analysis of embryos. To assess the effect of the procedure on early embryonic development, we generated a murine experimental system, including mice implanted with biopsied in vitro cultured embryos, control mice implanted with in vitro cultured embryos without biopsy, and mice with naturally conceived embryos. Embryos at the 7.5-dpc stage were isolated from all three groups and the embryo implantation rate, the survival rate of implanted embryos, and the developmental stage of surviving embryos were carefully assessed and compared among all three groups. We found the implantation rate was similar between biopsied and control group embryos (67.92% vs. 66.67%). However, the survival rate of implanted embryos in the biopsied group (49.31%) was significantly lower than that of the control (60.91%) and normal groups (96.24%) at 7.5 dpc. In addition, the survival rate of control group embryos was significant lower than that of normal group embryos. Classification of the precise developmental stages of randomly selected live implanted embryos at 7.5 dpc revealed no differences among the three groups. Our results indicate that blastomere biopsy does not adversely affect embryo implantation. The PGD procedure, in particular blastomere biopsy, increases the rate of embryo death at 4.5-7.5 dpc, but does not affect the development of surviving 7.5 dpc embryos.
authors.affiliation: State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.forename: Yan
authors.initials: Y
authors.lastname: Zeng
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Lv
authors.forename: Ru
authors.initials: R
authors.lastname: Wan
authors.forename: Man
authors.initials: M
authors.lastname: Tong
authors.forename: Hui
authors.initials: H
authors.lastname: Zhu
authors.forename: Liu
authors.initials: L
authors.lastname: Wang
authors.forename: Zuomin
authors.initials: Z
authors.lastname: Zhou
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhou
authors.forename: Jiahao
authors.initials: J
authors.lastname: Sha
mesh_terms: D000818:Animals; D001706:Biopsy; D001755:Blastocyst / Q000473:pathology*; D001757:Blastomeres / Q000473:pathology*; D010064:Embryo Implantation; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000473:pathology*; D005260:Female; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D011247:Pregnancy; D019836:Preimplantation Diagnosis / Q000401:mortality*; D015996:Survival Rate