title: Malformations of the great vessels in the neonatal rat induced by N-(2-aminoethyl)ethanolamine.
abstract: The reproductive and developmental toxicity of aminoethylethanolamine was evaluated in a standard screening study (OECD, 1995: Organisation for economic co-operation and development. Paris, France), in which groups of Wistar rats (10/sex/group) were administered the test substance by gavage at dosage levels of 50, 250, or 1000 mg/kg/day (groups 2-4, respectively). A control group received the vehicle, doubly distilled water. No live pups were delivered in group 4, and there was a higher incidence of stillborn offspring and reduced postnatal survival in group 3. Macroscopic changes in groups 2 and 3 were primarily related to the great vessels and characterized by dilations, aneurysms, and altered course of the aorta, pulmonary trunk, carotids, and the ductus arteriosus. A follow-up study was conducted to characterize the low dose-response, using dosage levels of 0, 0.2, 1, 5, or 50 mg/kg/day (groups 1-5, respectively). Given the expected scarcity of the lesions in control offspring, each group consisted of 25 animals of each sex. Macroscopic examination revealed a high incidence (18.5%) of aneurysm-bearing offspring in group 5 litters, and single offspring (0.3-0.4%) with aneurysms in groups 3 and 4. Microscopic examination revealed dissecting aneurysms in offspring from all aminoethylethanolamine treatment groups, without a clear dose-response between groups 2 and 4 (0.6%, 1.2%, and 0.3%, respectively), and focal hemorrhages in all groups including the control. In comparison, the background incidence of aneurysms in untreated 4-day old offspring was 0.2% (Treumann et al., 2011: Toxicol Pathol 39:969-974). Consequently, the findings in groups 2-4 cannot be conclusively attributed to treatment.
authors.affiliation: BASF SE, Ludwigshafen, Germany. steffen.schneider@basf.com
authors.forename: Steffen
authors.initials: S
authors.lastname: Schneider
authors.forename: Silke
authors.initials: S
authors.lastname: Treumann
authors.forename: Nigel P
authors.initials: NP
authors.lastname: Moore
mesh_terms: D000783:Aneurysm / Q000139:chemically induced / Q000473:pathology*; D000818:Animals; D000831:Animals, Newborn; D001808:Blood Vessels / Q000187:drug effects*; D001835:Body Weight; D002273:Carcinogens / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D004983:Ethanolamines / Q000633:toxicity*; D005260:Female; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012098:Reproduction / Q000187:drug effects*; D018675:Toxicity Tests; D054079:Vascular Malformations / Q000139:chemically induced / Q000473:pathology*
chemical_list: D002273:Carcinogens; D004983:Ethanolamines; C035835:N-(2-hydroxyethyl)ethylenediamine