title: Dielectrophoretic cell capture on polyester membranes.
abstract: A new system for dielectrophoretic cell capture on permeable polyester membranes is presented. Conventional photolithographic techniques were used to fabricate gold microelectrodes on a polyester membrane. The characterization of the microelectrodes showed that there were no differences regarding roughness, permeability, and hydrophilicity of the membrane before and after processing. Finally, dielectrophoretic cell capture and viability in a microfluidic device was demonstrated on the patterned membrane. These membranes could ultimately be combined with multilayer microfluidic devices to form a powerful tool for studies of cell-cell interactions in coculture, whereby spatial separation of different cell types and/or microenvironments are required.
authors.forename: Conni
authors.initials: C
authors.lastname: Hanke
authors.forename: Petra S
authors.initials: PS
authors.lastname: Dittrich
authors.forename: Darwin R
authors.initials: DR
authors.lastname: Reyes
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation*; D002477:Cells / Q000737:chemistry; D004586:Electrophoresis / Q000295:instrumentation*; D008567:Membranes, Artificial; D051379:Mice; D008839:Microelectrodes; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D041681:NIH 3T3 Cells; D011093:Polyethylene Terephthalates / Q000737:chemistry*
chemical_list: D008567:Membranes, Artificial; D011093:Polyethylene Terephthalates