title: Investigation of DNA damage treated with perfluorooctane sulfonate (PFOS) on ZrO2/DDAB active nano-order film.
abstract: The interactions between DNA and small molecules with planar heterocyclic structure were indicated in previous researches. This study investigated the interactions between PFOS with linear chain structure and DNA. A new phenomenon of DNA damage due to PFOS using electrochemistry technique was proved. The data was obtained on a modified glassy carbon electrode, on which didodecyldimethylammonium bromide (DDAB), ZrO(2) and calf thymus DNA were immobilized layer-by-layer. Electrochemical response of DNA damage caused by PFOS was detected by differential pulse voltammetry (DPV) using methylene blue (MB) as electro-active indicator. The current of MB attenuated obviously after DNA/ZrO(2)/DDAB/GCE were incubated in PFOS. The shift of MB reduction peak potential indicates that PFOS is bound with DNA in groove probably by the first step of hydrophobic interaction and then the second step of intercalation into the base of DNA. X-ray photoelectron spectroscopic (XPS) was used to elucidate in detail the intercalation of PFOS into DNA and the formation of hydrogen bond between PFOS and DNA base. Electrochemical quartz crystal microbalance (EQCM) proved the formation of adducts of DNA and PFOS. Moreover, electrochemical impedance spectroscopy (EIS) indicates that the PFOS influence DNA structure and attenuate DNA charge transport. These results demonstrate that PFOS intercalated into DNA do induce DNA base damage.
authors.affiliation: College of Environmental and Energy Engineering, Beijing University of Technology, Beijing 100124, China. Electronic address: lipinglu@bjut.edu.cn.
authors.forename: Liping
authors.initials: L
authors.lastname: Lu
authors.affiliation: College of Environmental and Energy Engineering, Beijing University of Technology, Beijing 100124, China.
authors.forename: Laihui
authors.initials: L
authors.lastname: Xu
authors.affiliation: College of Environmental and Energy Engineering, Beijing University of Technology, Beijing 100124, China.
authors.forename: Tianfang
authors.initials: T
authors.lastname: Kang
authors.affiliation: College of Environmental and Energy Engineering, Beijing University of Technology, Beijing 100124, China.
authors.forename: Shuiyuan
authors.initials: S
authors.lastname: Cheng
mesh_terms: D017738:Alkanesulfonic Acids / Q000737:chemistry / Q000633:toxicity*; D000818:Animals; D002417:Cattle; D018736:DNA Adducts; D004249:DNA Damage; D058266:Dielectric Spectroscopy; D004785:Environmental Pollutants / Q000737:chemistry / Q000633:toxicity; D005466:Fluorocarbons / Q000737:chemistry / Q000633:toxicity*; D006860:Hydrogen Bonding; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007364:Intercalating Agents / Q000633:toxicity; D008051:Lipid Bilayers; D053768:Metal Nanoparticles / Q000737:chemistry; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry; D056951:Photoelectron Spectroscopy; D058525:Quartz Crystal Microbalance Techniques / Q000379:methods*; D000644:Quaternary Ammonium Compounds; D015040:Zirconium
chemical_list: D017738:Alkanesulfonic Acids; D018736:DNA Adducts; D004785:Environmental Pollutants; D005466:Fluorocarbons; D007364:Intercalating Agents; D008051:Lipid Bilayers; D000644:Quaternary Ammonium Compounds; C046112:didodecyldimethylammonium; C076994:perfluorooctane sulfonic acid; D015040:Zirconium; C028541:zirconium oxide