title: Analysis of protein interactions with immobilized Peptide arrays synthesized on membrane supports.
abstract: INTRODUCTIONThe following protocol describes the synthesis of short linear peptides, or peptide pools, on modified cellulose membranes, and the detection of their protein-binding partners. Peptides are synthesized from their carboxyl termini using Fmoc-amino acid derivatives. After completion of the synthesis and cleavage of all side-chain-protecting groups, the peptide array on the membrane is incubated with the potential interaction partners to identify their target sequences.
authors.forename: Ronald
authors.initials: R
authors.lastname: Frank
authors.forename: Stefan
authors.initials: S
authors.lastname: Dübel