title: Over-expression of Hlx homeobox gene in DC2.4 dendritic cell enhances its maturation and antigen presentation.
abstract: Hlx as a Th1-specific transcription factor, it appears to drive maturation of Th1 and IFN-γ secretion in cooperation with T-bet. In this study, we established a stable Hlx-over-expressed dendritic cell line (DC2.4/Hlx), and investigated the possible effect of Hlx gene on maturation of dendritic cell-line (DC2.4). Results shown that over-expressed Hlx in DC2.4 up-regulated the transcription and expression of IFN-γ, increased the expression of maturation makers including CD40, CD80, CD86, MHC-I and MHC-II. Functional assay for DC2.4/Hlx showed that over-expressed Hlx increased the expression level of interleukin-12 in the supernatant and decreased DC endocytosis when cells were incubated in vitro. Furthermore, using a syngeneic T cell activation model, we found that DC2.4/Hlx could obviously present ovalbumin (OVA) antigen to T cell in OVA pre-immunized mice.
authors.affiliation: Department of Immunology, Institute of Laboratory Medicine, Jiangsu University, Zhenjiang, PR China.
authors.forename: Siamak Sandoghchian
authors.initials: SS
authors.lastname: Shotorbani
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: He
authors.forename: Heng
authors.initials: H
authors.lastname: Yang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Sun
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.forename: Zhaoliang
authors.initials: Z
authors.lastname: Su
authors.forename: Yuan
authors.initials: Y
authors.lastname: Xue
authors.forename: Dong
authors.initials: D
authors.lastname: Zheng
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Shengjun
authors.initials: S
authors.lastname: Wang
authors.forename: Qixiang
authors.initials: Q
authors.lastname: Shao
authors.forename: Liwei
authors.initials: L
authors.lastname: Lu
authors.forename: Huaxi
authors.initials: H
authors.lastname: Xu
mesh_terms: D000818:Animals; D017951:Antigen Presentation; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D002460:Cell Line; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D005786:Gene Expression Regulation; D018398:Homeodomain Proteins / Q000235:genetics / Q000276:immunology*; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism; D018664:Interleukin-12 / Q000235:genetics / Q000276:immunology; D051379:Mice; D014157:Transcription Factors / Q000235:genetics / Q000276:immunology*
chemical_list: D015415:Biomarkers; C489999:Hlx protein, mouse; D018398:Homeodomain Proteins; D014157:Transcription Factors; D018664:Interleukin-12; D007371:Interferon-gamma