title: Novel fluorescence method for detection of α-L-fucosidase based on CdTe quantum dots.
abstract: The enzyme α-L-fucosidase (AFu) plays an important role in the diagnosis of hepatocellular carcinoma (HCC) and fucosidosis. In this paper, a simple, sensitive and precise method based upon measuring the fluorescence quenching of CdTe semiconductor quantum dots (QDs) was developed for detecting the enzymatic activity of AFu. The detection limit of AFu was 0.01 U/L (n = 3) and the linear relationship was 0.01-4 U/L. The selectivity experiment indicated excellent selectivity for AFu over a number of interfering species. We have also studied the detection mechanism of AFu by X-ray photoelectron spectroscopy (XPS) and found that the quenching effect was caused by the oxidation of tellurium by 2-chloro-4-nitrophenol (2-CNP) which produced in AFu catalytic reaction. Moreover, the AFu sensor based on QDs was used satisfactorily for the assessment of AFu activity in serum samples. It will most probably be applicable in assembling diagnostic microdevice to realize the rapid clinic analysis of AFu.
authors.affiliation: Laboratory of Controllable Preparation and Application of Nanomaterials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190, People's Republic of China.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Chen
authors.forename: Xiangling
authors.initials: X
authors.lastname: Ren
authors.forename: Xianwei
authors.initials: X
authors.lastname: Meng
authors.forename: Yanqi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Dong
authors.initials: D
authors.lastname: Chen
authors.forename: Fangqiong
authors.initials: F
authors.lastname: Tang
mesh_terms: D019187:Cadmium Compounds / Q000737:chemistry*; D006528:Carcinoma, Hepatocellular / Q000097:blood; D005645:Fucosidosis / Q000097:blood; D006801:Humans; D057230:Limit of Detection; D008113:Liver Neoplasms / Q000097:blood; D045663:Quantum Dots; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013691:Tellurium / Q000737:chemistry*; D005644:alpha-L-Fucosidase / Q000032:analysis / Q000097:blood*
chemical_list: D019187:Cadmium Compounds; D005644:alpha-L-Fucosidase; D013691:Tellurium; C028337:cadmium telluride