title: Universal quantification of structurally diverse natural products using an evaporative light scattering detector.
abstract: A lack of good methods for absolute quantification of natural products has limited the accuracy of high-throughput screening. Many currently used methods for quantification are either too slow or not amenable to the structural diversity of natural products. Recent developments in low-temperature evaporative light scattering detectors (ELSD-LT) have overcome several historical limitations of ELSDs, including analyte decomposition and low sensitivity. Primarily, ELSDs have been used for relative quantification and detection of compounds that lack a UV chromophore. In this study, we employ an ELSD-LT for absolute quantification of natural products. Calibration curves were constructed using a weighted least-squares analysis for a diverse set of natural products and other compounds. An average calibration curve was evaluated for the "universal" quantification of natural products. Optimization of ELSD-LT hardware and parameters improved sensitivity and throughput and established the utility of ELSD-LT for quantification of large natural product libraries.
authors.affiliation: Pharmaceutical Sciences Division, University of Wisconsin-Madison, 777 Highland Avenue, Madison, Wisconsin 53705, USA.
authors.forename: Navid
authors.initials: N
authors.lastname: Adnani
authors.forename: Cole R
authors.initials: CR
authors.lastname: Michel
authors.forename: Tim S
authors.initials: TS
authors.lastname: Bugni
mesh_terms: D000465:Algorithms; D001688:Biological Products / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation; D008027:Light; D015394:Molecular Structure; D012542:Scattering, Radiation
chemical_list: D001688:Biological Products