title: Composition and dynamics of biostimulated indigenous oil-degrading microbial consortia from the Irish, North and Mediterranean Seas: a mesocosm study.
abstract: Diversity of indigenous microbial consortia and natural occurrence of obligate hydrocarbon-degrading bacteria (OHCB) are of central importance for efficient bioremediation techniques. To investigate the microbial population dynamics and composition of oil-degrading consortia, we have established a series of identical oil-degrading mesocosms at three different locations, Bangor (Menai Straits, Irish Sea), Helgoland (North Sea) and Messina (Messina Straits, Mediterranean Sea). Changes in microbial community composition in response to oil spiking, nutrient amendment and filtration were assessed by ARISA and DGGE fingerprinting and 16Sr RNA gene library analysis. Bacterial and protozoan cell numbers were quantified by fluorescence microscopy. Very similar microbial population sizes and dynamics, together with key oil-degrading microorganisms, for example, Alcanivorax borkumensis, were observed at all three sites; however, the composition of microbial communities was largely site specific and included variability in relative abundance of OHCB. Reduction in protozoan grazing had little effect on prokaryotic cell numbers but did lead to a decrease in the percentage of A. borkumensis 16S rRNA genes detected in clone libraries. These results underline the complexity of marine oil-degrading microbial communities and cast further doubt on the feasibility of bioaugmentation practices for use in a broad range of geographical locations.
authors.affiliation: School of Biological Sciences, Environment Centre for Wales, Bangor University, Bangor, Gwynedd, UK. c.gertler@bangor.ac.uk
authors.forename: Christoph
authors.initials: C
authors.lastname: Gertler
authors.forename: Daniela J
authors.initials: DJ
authors.lastname: Näther
authors.forename: Simone
authors.initials: S
authors.lastname: Cappello
authors.forename: Gunnar
authors.initials: G
authors.lastname: Gerdts
authors.forename: Richard S
authors.initials: RS
authors.lastname: Quilliam
authors.forename: Michail M
authors.initials: MM
authors.lastname: Yakimov
authors.forename: Peter N
authors.initials: PN
authors.lastname: Golyshin
mesh_terms: D053521:Alcanivoraceae / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001673:Biodegradation, Environmental; D016798:Ciliophora / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism; D056890:Eukaryota / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism; D005060:Europe; D008522:Mediterranean Sea; D059013:Microbial Consortia; D009659:North Sea; D009792:Oceans and Seas; D010578:Petroleum / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics
chemical_list: D010578:Petroleum; D012336:RNA, Ribosomal, 16S