title: An ethanol extract of Artemisia iwayomogi activates PPARδ leading to activation of fatty acid oxidation in skeletal muscle.
abstract: Although Artemisia iwayomogi (AI) has been shown to improve the lipid metabolism, its mode of action is poorly understood. In this study, a 95% ethanol extract of AI (95EEAI) was identified as a potent ligand of peroxisome proliferator-activated receptorδ (PPARδ) using ligand binding analysis and cell-based reporter assay. In cultured primary human skeletal muscle cells, treatment of 95EEAI increased expression of two important PPARδ-regulated genes, carnitine palmitoyl-transferase-1 (CPT1) and pyruvate dehydrogenase kinase isozyme 4 (PDK4), and several genes acting in lipid efflux and energy expenditure. Furthermore, 95EEAI stimulated fatty acid oxidation in a PPARδ-dependent manner. High-fat diet-induced obese mice model further indicated that administration of 95EEAI attenuated diet-induced obesity through the activation of fatty acid oxidation in skeletal muscle. These results suggest that a 95% ethanol extract of AI may have a role as a new functional food material for the prevention and/or treatment of hyperlipidermia and obesity.
authors.affiliation: Health Science Research Institute, Research and Development Center, AmorePacific Corporation, Bora-dong, Giheung-gu, Yongin-si, Korea.
authors.forename: Si Young
authors.initials: SY
authors.lastname: Cho
authors.forename: Hyun Woo
authors.initials: HW
authors.lastname: Jeong
authors.forename: Jong Hee
authors.initials: JH
authors.lastname: Sohn
authors.forename: Dae-Bang
authors.initials: DB
authors.lastname: Seo
authors.forename: Wan Gi
authors.initials: WG
authors.lastname: Kim
authors.forename: Sang-Jun
authors.initials: SJ
authors.lastname: Lee
mesh_terms: D000818:Animals; D019306:Artemisia / Q000737:chemistry*; D059305:Diet, High-Fat / Q000009:adverse effects; D000431:Ethanol / Q000737:chemistry; D005227:Fatty Acids / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008024:Ligands; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism*; D009765:Obesity / Q000188:drug therapy / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D047494:PPAR delta / Q000378:metabolism*; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D011485:Protein Binding; D015533:Transcriptional Activation / Q000187:drug effects
chemical_list: D005227:Fatty Acids; D008024:Ligands; D047494:PPAR delta; D010936:Plant Extracts; D000431:Ethanol; D005947:Glucose