title: Efficient synthesis of a chiral precursor for angiotensin-converting enzyme (ACE) inhibitors in high space-time yield by a new reductase without external cofactors.
abstract: A new reductase, CgKR2, with the ability to reduce ethyl 2-oxo-4-phenylbutyrate (OPBE) to ethyl (R)-2-hydroxy-4-phenylbutyrate ((R)-HPBE), an important chiral precursor for angiotensin-converting enzyme (ACE) inhibitors, was discovered. For the first time, (R)-HPBE with >99% ee was produced via bioreduction of OPBE at 1 M without external addition of cofactors. The space-time yield (700 g·L(-1)·d(-1)) was 27 times higher than the highest record.
authors.affiliation: Laboratory of Biocatalysis and Synthetic Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, People's Republic of China.
authors.forename: Nai-Dong
authors.initials: ND
authors.lastname: Shen
authors.forename: Yan
authors.initials: Y
authors.lastname: Ni
authors.forename: Hong-Min
authors.initials: HM
authors.lastname: Ma
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Wang
authors.forename: Chun-Xiu
authors.initials: CX
authors.lastname: Li
authors.forename: Gao-Wei
authors.initials: GW
authors.lastname: Zheng
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Jian-He
authors.initials: JH
authors.lastname: Xu
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D002175:Candida / Q000201:enzymology; D004926:Escherichia coli / Q000187:drug effects; D015394:Molecular Structure; D010088:Oxidoreductases / Q000378:metabolism*; D042084:Paracoccus pantotrophus / Q000201:enzymology; D010654:Phenylbutyrates / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D013237:Stereoisomerism
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D010654:Phenylbutyrates; C572920:ethyl (R)-2-hydroxy-4-phenylbutyrate; C572921:ethyl 2-oxo-4-phenylbutyrate; D010088:Oxidoreductases