title: Mus spicilegus endogenous retrovirus HEMV uses murine sodium-dependent myo-inositol transporter 1 as a receptor.
abstract: We sought to determine the relationship between two recent additions to the murine leukemia virus (MLV) ecotropic subgroup: Mus cervicolor isolate M813 and Mus spicilegus endogenous retrovirus HEMV. Though divergent in sequence, the two viruses share an Env protein with similarly curtailed VRA and VRB regions, and infection by both is restricted to mouse cells. HEMV and M813 displayed reciprocal receptor interference, suggesting that they share a receptor. Expression of the M813 receptor murine sodium-dependent myo-inositol transporter 1 (mSMIT1) allowed previously nonpermissive cells to be infected by HEMV, indicating that mSMIT1 also serves as a receptor for HEMV. Our findings add HEMV as a second member to the MLV subgroup that uses mSMIT1 to gain entry into cells.
authors.affiliation: Department of Molecular Biology and Microbiology, Tufts University, Boston, Massachusetts, USA.
authors.forename: Christopher H
authors.initials: CH
authors.lastname: Tipper
authors.forename: Oya
authors.initials: O
authors.lastname: Cingöz
authors.forename: John M
authors.initials: JM
authors.lastname: Coffin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D020077:Endogenous Retroviruses / Q000502:physiology*; D009052:Leukemia Virus, Murine / Q000502:physiology*; D051379:Mice; D008969:Molecular Sequence Data; D011991:Receptors, Virus / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D027981:Symporters; D014759:Viral Envelope Proteins / Q000235:genetics; D053586:Virus Internalization
chemical_list: D011991:Receptors, Virus; C508355:SLC5A3 protein, mouse; D027981:Symporters; D014759:Viral Envelope Proteins