title: Gas-filled phospholipid nanoparticles conjugated with gadolinium play a role as a potential theragnostics for MR-guided HIFU ablation.
abstract: To develop a long-circulating theragnostics, meaning therapeutics and diagnostics for MR-guided HIFU ablation, we designed and prepared Gd-C(5)F(12)-phospholipid nanobubbles (PLNs) 30-100 nm in diameter. The biochemical and physical characterization of Gd-C(5)F(12)-PLNs were performed. Since Gd-C(5)F(12)-PLN-50 (Φ = 50 nm) and Gd-C(5)F(12)-PLN-100 (Φ = 100 nm) enhanced the hyperthermal effect of HIFU size- and concentration-dependently in a tissue-mimicking phantom, its circulation, distribution, tumor accumulation and tumor ablation were examined in tumor-bearing mice. The plasma-half life of Gd-C(5)F(12)-PLNs was longer than 1.5 hrs. Gd-C(5)F(12)-PLNs mainly accumulated in the liver and the spleen, suggesting that they are slowly secreted through the hepatobiliary pathway. Monitored by the T1 signal intensity of MR, Gd-C(5)F(12)-PLNs accumulated in tumor tissues for 8 hours in mice. HIFU with Gd-C(5)F(12)-PLN-100 showed the increased tumor ablation area as compared with HIFU alone. The results suggest that Gd-C(5)F(12)-PLNs exhibit a potential theragnostics for MR-guided HIFU ablation.
authors.affiliation: Molecular and Cellular Imaging Center, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Kangnam-gu, Seoul, South Korea.
authors.forename: Se-Young
authors.initials: SY
authors.lastname: Choi
authors.forename: Young-Sun
authors.initials: YS
authors.lastname: Kim
authors.forename: Yeong-Ju
authors.initials: YJ
authors.lastname: Seo
authors.forename: Jehoon
authors.initials: J
authors.lastname: Yang
authors.forename: Kyu-Sil
authors.initials: KS
authors.lastname: Choi
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D003287:Contrast Media / Q000494:pharmacology; D005682:Gadolinium / Q000737:chemistry*; D019786:Gadolinium DTPA / Q000737:chemistry; D005740:Gases; D057086:High-Intensity Focused Ultrasound Ablation / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000737:chemistry*; D009369:Neoplasms / Q000628:therapy*; D019047:Phantoms, Imaging; D010743:Phospholipids / Q000737:chemistry*; D010758:Phosphorus / Q000737:chemistry; D013778:Tetrazolium Salts / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology; D016896:Treatment Outcome
chemical_list: D001672:Biocompatible Materials; D003287:Contrast Media; D005740:Gases; D010743:Phospholipids; D013778:Tetrazolium Salts; D013844:Thiazoles; D010758:Phosphorus; D005682:Gadolinium; C022616:thiazolyl blue; D019786:Gadolinium DTPA