title: Characterization of Bombyx mori nucleopolyhedrovirus with a knockout of Bm17.
abstract: Open reading frame 17 (Bm17) gene of Bombyx mori nucleopolyhedrovirus is a highly conserved gene in lepidopteran nucleopolyhedroviruses, but its function remains unknown. In this report, transient-expression and superinfection assays indicated that BM17 localized in the nucleus and cytoplasm of infected BmN cells. To determine the role of Bm17 in baculovirus life cycle, we constructed a Bm17 knockout virus and characterized its properties in cells. Analysis of the production and infection of budded virions, the level of viral DNA replication revealed showed that there was no significant difference among the mutant, the control, and the Bm17 repaired virus strains. These results suggest that BM17 is not essential for virus replication in cultured cells.
authors.affiliation: School of Medical Science and Laborarory Medicine, Jiangsu University, Zhenjiang, 212013, People's Republic of China.
authors.forename: Hongxing
authors.initials: H
authors.lastname: Shen
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhou
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.forename: Bin
authors.initials: B
authors.lastname: Nin
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Wang
authors.forename: Shihe
authors.initials: S
authors.lastname: Shao
authors.forename: Huiqing
authors.initials: H
authors.lastname: Chen
authors.forename: Zhongjian
authors.initials: Z
authors.lastname: Guo
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Liu
authors.forename: Qin
authors.initials: Q
authors.lastname: Yao
authors.forename: Keping
authors.initials: K
authors.lastname: Chen