title: Characteristics of alloreactive T cells measured before renal transplantation.
abstract: Several assays to measure pre-existing allospecific T cell immunity in renal transplant candidates have been developed in the past years. In 46 patients, we used flow cytometry-based mixed lymphocyte culture to measure the precursor frequency and phenotype of alloreactive T cells before renal transplantation, using donor-specific or third-party cells for allostimulation. Allostimulation induced up-regulation of co-stimulatory molecules, chemokine receptors relevant for migration of T cells into the graft and effector proteins. Recipients prone for acute rejection had a higher precursor frequency of alloreactive CD8(+)  T cells and a lower percentage of interleukin (IL)-7Rα expressing alloreactive CD8(+)  T cells than non-rejectors. These data point to quantitative and qualitative differences between T cells of patients who will experience acute cellular rejection episodes from those who will not.
authors.affiliation: Renal Transplant Unit, Department of Experimental Immunology, Academic Medical Center, Amsterdam, the Netherlands. p.j.vandeberg@amc.nl
authors.forename: P J E J
authors.initials: PJ
authors.lastname: van de Berg
authors.forename: S L
authors.initials: SL
authors.lastname: Yong
authors.forename: S D
authors.initials: SD
authors.lastname: Koch
authors.forename: N
authors.initials: N
authors.lastname: Lardy
authors.forename: K A M I
authors.initials: KA
authors.lastname: van Donselaar-van der Pant
authors.forename: S
authors.initials: S
authors.lastname: Florquin
authors.forename: F J
authors.initials: FJ
authors.lastname: Bemelman
authors.forename: R A W
authors.initials: RA
authors.lastname: van Lier
authors.forename: I J M
authors.initials: IJ
authors.lastname: ten Berge
mesh_terms: D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D005260:Female; D006084:Graft Rejection / Q000188:drug therapy / Q000276:immunology; D006801:Humans; D016130:Immunophenotyping; D007371:Interferon-gamma / Q000378:metabolism; D053720:Interleukin-15 Receptor alpha Subunit / Q000378:metabolism; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism; D016030:Kidney Transplantation / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology; D018655:Lymphocyte Count; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D008875:Middle Aged; D019707:Receptors, Chemokine / Q000378:metabolism; D018121:Receptors, Cytokine / Q000378:metabolism; D020395:Receptors, Interleukin-7 / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology*; D014019:Tissue Donors; D014184:Transplantation, Homologous / Q000276:immunology
chemical_list: D053720:Interleukin-15 Receptor alpha Subunit; D053645:Interleukin-2 Receptor alpha Subunit; D019707:Receptors, Chemokine; D018121:Receptors, Cytokine; D020395:Receptors, Interleukin-7; C415350:interleukin-7 receptor, alpha chain; D007371:Interferon-gamma