title: The facile synthesis of multifunctional PAMAM dendrimer conjugates through copper-free click chemistry.
abstract: The facile conjugation of three azido modified functionalities, namely a therapeutic drug (methotrexate), a targeting moiety (folic acid), and an imaging agent (fluorescein) with a G5 PAMAM dendrimer scaffold with cyclooctyne molecules at the surface through copper-free click chemistry is reported. Mono-, di-, and tri-functional PAMAM dendrimer conjugates can be obtained via combinatorial mixing of different azido modified functionalities simultaneously or sequentially with the dendrimer platform. Preliminary flow cytometry results indicate that the folic acid targeted nanoparticles are efficiently binding with KB cells.
authors.affiliation: Michigan Nanotechnology Institute for Medicine and Biological Sciences, University of Michigan, 9220 MSRB III, Box 0648, Ann Arbor, MI 48109, USA. baohua@med.umich.edu
authors.forename: Baohua
authors.initials: B
authors.lastname: Huang
authors.forename: Jolanta F
authors.initials: JF
authors.lastname: Kukowska-Latallo
authors.forename: Shengzhuang
authors.initials: S
authors.lastname: Tang
authors.forename: Hong
authors.initials: H
authors.lastname: Zong
authors.forename: Kali B
authors.initials: KB
authors.lastname: Johnson
authors.forename: Ankur
authors.initials: A
authors.lastname: Desai
authors.forename: Chris L
authors.initials: CL
authors.lastname: Gordon
authors.forename: Pascale R
authors.initials: PR
authors.lastname: Leroueil
authors.forename: James R
authors.initials: JR
authors.lastname: Baker
mesh_terms: D001386:Azides; D057930:Click Chemistry / Q000379:methods*; D003300:Copper; D050091:Dendrimers / Q000138:chemical synthesis*; D016503:Drug Delivery Systems / Q000379:methods*; D019793:Fluorescein / Q000737:chemistry; D005492:Folic Acid / Q000737:chemistry; D006801:Humans; D007624:KB Cells; D008727:Methotrexate / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry
chemical_list: D001386:Azides; D050091:Dendrimers; C104700:PAMAM Starburst; D003300:Copper; D005492:Folic Acid; D019793:Fluorescein; D008727:Methotrexate