title: Serological analysis of xenogeneic anti-lymphoblastoid cell-line sera with specificity against HLA-B12.
abstract: In view of the importance of potent anti-HLA sera with narrow reaction patterns against defined HLA antigens, two xenogeneic antisera were raised in rabbits following immunization with human lymphoblastoid cell lines from HLA-nonidentical donors homozygous for HLA-B12. After absorption with lymphoblastoid cell lines of an appropriate HLA phenotype, the antisera were purified over DEAE-cellulose ion exchange chromatography and reconcentrated. Both antisera recognized HLA-B12-positive peripheral blood cells of unrelated donors tested in the microcytotoxicity assay. The two rabbit antisera revealed a high degree of similarity in their anti-HLA-B12 antibody specificity. One antiserum showed some cross reactivity with HLA-B13 as has been reported in allo-anti-HLA-B12 sera. The other antiserum revealed some activity against HLA-DRw7-positive donors. Antibody activity could be removed completely from two further rabbit anti-HLA antisera by absorption with lymphoblastoid cell lines from related and unrelated HLA-identical donors. The advantages of using lymphoblastoid cell lines as immunogens and absorption material for the production of heterologous anti-HLA typing sera are discussed.
authors.affiliation: Institut für Hämatologie, Abteilung Immunologie, GSF München, Munich, Federal Republik of Germany.
authors.forename: K
authors.initials: K
authors.lastname: Schäfer
authors.forename: E D
authors.initials: ED
authors.lastname: Albert
authors.forename: H
authors.initials: H
authors.lastname: Rodt
authors.forename: S
authors.initials: S
authors.lastname: Thierfelder
mesh_terms: D000818:Animals; D000910:Antibodies, Heterophile / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology*; D000961:Antilymphocyte Serum / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D002460:Cell Line; D003429:Cross Reactions; D003601:Cytotoxicity Tests, Immunologic; D006680:HLA Antigens / Q000276:immunology*; D015235:HLA-B Antigens / Q000097:blood / Q000276:immunology*; D059908:HLA-B13 Antigen / Q000276:immunology*; D006684:HLA-DR Antigens / Q000276:immunology*; D006650:Histocompatibility Testing; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D011817:Rabbits; D061349:Unrelated Donors
chemical_list: D000910:Antibodies, Heterophile; D000961:Antilymphocyte Serum; D006680:HLA Antigens; D015235:HLA-B Antigens; C035015:HLA-B12 antigen; D059908:HLA-B13 Antigen; D006684:HLA-DR Antigens; D007106:Immune Sera