title: Role of Aspergillus niger acrA in arsenic resistance and its use as the basis for an arsenic biosensor.
abstract: Arsenic contamination of groundwater sources is a major issue worldwide, since exposure to high levels of arsenic has been linked to a variety of health problems. Effective methods of detection are thus greatly needed as preventive measures. In an effort to develop a fungal biosensor for arsenic, we first identified seven putative arsenic metabolism and transport genes in Aspergillus niger, a widely used industrial organism that is generally regarded as safe (GRAS). Among the genes tested for RNA expression in response to arsenate, acrA, encoding a putative plasma membrane arsenite efflux pump, displayed an over 200-fold increase in gene expression in response to arsenate. We characterized the function of this A. niger protein in arsenic efflux by gene knockout and confirmed that AcrA was located at the cell membrane using an enhanced green fluorescent protein (eGFP) fusion construct. Based on our observations, we developed a putative biosensor strain containing a construct of the native promoter of acrA fused with egfp. We analyzed the fluorescence of this biosensor strain in the presence of arsenic using confocal microscopy and spectrofluorimetry. The biosensor strain reliably detected both arsenite and arsenate in the range of 1.8 to 180 μg/liter, which encompasses the threshold concentrations for drinking water set by the World Health Organization (10 and 50 μg/liter).
authors.affiliation: Department of Microbiology and Immunology, McGill University, Montréal, Québec, Canada .
authors.forename: Se-In
authors.initials: SI
authors.lastname: Choe
authors.forename: Fabrice N
authors.initials: FN
authors.lastname: Gravelat
authors.forename: Qusai
authors.initials: Q
authors.lastname: Al Abdallah
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Lee
authors.forename: Bernard F
authors.initials: BF
authors.lastname: Gibbs
authors.forename: Donald C
authors.initials: DC
authors.lastname: Sheppard
mesh_terms: D001151:Arsenic / Q000378:metabolism* / Q000494:pharmacology*; D018053:Arsenites / Q000378:metabolism; D001234:Aspergillus niger / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D002462:Cell Membrane / Q000378:metabolism; D060766:Drinking Water / Q000737:chemistry; D025141:Drug Resistance, Fungal; D005456:Fluorescent Dyes / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D060587:Groundwater / Q000737:chemistry; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D018053:Arsenites; D060766:Drinking Water; D005456:Fluorescent Dyes; D005656:Fungal Proteins; D011993:Recombinant Fusion Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; C015001:arsenite; D001151:Arsenic