title: Molecular characterization of a recent Newcastle disease virus outbreak in Jordan.
abstract: Newcastle disease (ND) is a highly contagious viral disease and is a continuous threat to the poultry industry worldwide. In the early months of 2011, several devastating ND outbreaks occurred in Jordan affecting broilers, layers and breeders. The fusion gene of the isolated Newcastle disease virus (NDV) was partially amplified by RT-PCR, then directly sequenced. The NDV isolates were found to have the motif112RRQKRF117. This motif and a mean death time (MDT) of 46 h are indicative of the velogenic nature of these NDV isolates. Phylogenetic analysis showed that the new NDV strain belongs to the lineage 5d (Aldous et al., 2003) and is closely related to the Chinese strain SG/Liaoning/2009. NDV outbreaks in 2010 and 2011 have been noted in neighboring countries. Based on the high nucleotide similarity between our isolated NDV isolates and the Chinese NDV strain, the origin of these recent NDV isolates might be from China.
authors.affiliation: Faculty of Veterinary Medicine, Jordan University of Science and Technology, P.O. Box 3030, Irbid 22110, Jordan. ababnem@just.edu.jo
authors.forename: Mustafa M K
authors.initials: MM
authors.lastname: Ababneh
authors.forename: Abd Elhafeed
authors.initials: AE
authors.lastname: Dalab
authors.forename: Saad R
authors.initials: SR
authors.lastname: Alsaad
authors.forename: Mohammad B
authors.initials: MB
authors.lastname: Al-Zghoul
authors.forename: Mohammad Q
authors.initials: MQ
authors.lastname: Al-Natour
mesh_terms: D000818:Animals; D002645:Chickens; D004196:Disease Outbreaks / Q000662:veterinary*; D007597:Jordan / Q000453:epidemiology; D017720:Molecular Epidemiology; D009521:Newcastle Disease / Q000453:epidemiology / Q000821:virology*; D009522:Newcastle disease virus / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification
chemical_list: D012367:RNA, Viral