title: A simplified PCR method for genotyping Mycoplasma mycoides subspecies mycoides small colony: the aetiologic agent of contagious bovine pleuropneumonia.
abstract: Mycoplasma mycoides subspecies mycoides small colony is the aetiological agent of contagious bovine pleuropneumonia, a cattle disease endemic to areas of sub-Saharan Africa. Twenty isolates from various geographical locations and the type strain were analysed by multi-locus sequence analysis (MLSA). The data generated was then used to develop three PCR primer sets to differentiate these isolates. The PCRs differentiated the isolates into four groups; the type strain (T); isolates of European origin (Eu); isolates from Tanzania (Af1) with a final group consisting of isolates from Namibia and Botswana (Af2). These PCRs offers a rapid and efficient post-identification typing method without the need to sequence and analyse multiple genes.
authors.affiliation: Mycoplasma Group, Department of Bacteriology, Animal Health and Veterinary Laboratories Agency, Surrey KT15 3NB, UK. colin.churchward@ahvla.gsi.gov.uk
authors.forename: Colin P
authors.initials: CP
authors.lastname: Churchward
authors.forename: Miroslav
authors.initials: M
authors.lastname: Hlúšek
authors.forename: Robin A J
authors.initials: RA
authors.lastname: Nicholas
authors.forename: Roger D
authors.initials: RD
authors.lastname: Ayling
authors.forename: Laura
authors.initials: L
authors.lastname: McAuliffe
mesh_terms: D017954:Africa South of the Sahara; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000382:microbiology*; D017931:DNA Primers / Q000235:genetics; D005838:Genotype; D060005:Genotyping Techniques / Q000662:veterinary*; D008969:Molecular Sequence Data; D009176:Mycoplasma mycoides / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D011002:Pleuropneumonia, Contagious / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide
chemical_list: D017931:DNA Primers