title: Association between INS-VNTR polymorphism and polycystic ovary syndrome in a Korean population.
abstract: Polycystic ovary syndrome (PCOS) is a common disorder in women of reproductive ages. But its etiology is not fully understood yet. Variability in the number of tandem repeats of the insulin gene (INS-VNTR) is known to associate with PCOS, and it is associated with an increased risk of diabetes mellitus and other cardiovascular diseases. The aim of our study was to analyze an association between the INS-VNTR polymorphism and PCOS in a Korean population. The -23/Hph I polymorphism was used as a surrogate marker for INS-VNTR polymorphism and a total of 218 PCOS patient and 141 control DNAs were analyzed by restriction fragment length polymorphism method. Statistical analysis of genotyping results were performed using HapAnalyzer. χ² test and logistic regression were used to analyze the association between two groups. A p value <0.05 was considered statistically significant. The frequencies of A/A and A/T genotypes indicated a similar change between PCOS patients and controls. In conclusion, there was no association between PCOS and INS-VNTR polymorphism (p = 0.0544, odds ratio = 1.69). Our present data demonstrate that INS-VNTR polymorphism is not related with PCOS in Korean women. Thus, it is suggested that INS-VNTR polymorphism is not a key factor in the etiology and the pathogenesis of PCOS in a Korean population.
authors.affiliation: Department of Biomedical Science, CHA Stem Cell Institute, CHA University, Bundang CHA Hospital, Gyeonggi-Do, Korea.
authors.forename: Ji-Hyun
authors.initials: JH
authors.lastname: Yun
authors.forename: Bon-Hee
authors.initials: BH
authors.lastname: Gu
authors.forename: Yu-Bin
authors.initials: YB
authors.lastname: Kang
authors.forename: Bum-Chae
authors.initials: BC
authors.lastname: Choi
authors.forename: Sangjin
authors.initials: S
authors.lastname: Song
authors.forename: Kwang-Hyun
authors.initials: KH
authors.lastname: Baek
mesh_terms: D024506:5' Flanking Region; D000328:Adult; D054458:Amplified Fragment Length Polymorphism Analysis; D044466:Asian People; D016022:Case-Control Studies; D005260:Female; D056726:Genetic Association Studies; D005819:Genetic Markers; D006579:Heterozygote; D006720:Homozygote; D006769:Hospitals, General; D006801:Humans; D007328:Insulin / Q000235:genetics* / Q000378:metabolism; D018598:Minisatellite Repeats; D011085:Polycystic Ovary Syndrome / Q000235:genetics* / Q000378:metabolism; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D056910:Republic of Korea
chemical_list: D005819:Genetic Markers; D007328:Insulin