title: Edaravone suppresses postoperative reperfusion injury in rat lower extremity: An immunohistological study.
abstract: OBJECTIVE
  The present study evaluated whether the free radical scavenger edaravone (Radicut [Mitsubishi Pharma Co, Japan]) can suppress lower extremity postoperative reperfusion injury by evaluating muscle cell viability with immunohistological stain (cytochrome c oxidase stain).
  
  
  METHODS
  Eight Lewis male rats (460 g to 510 g) were divided into two groups. In the control group, postoperative reperfusion injury models were created by clamping the bilateral common femoral arteries for 5 h and then releasing. In the other group, 9.0 mg/kg of edaravone was administered before clamping the bilateral common femoral arteries. After 5 h of reperfusion, the bilateral triceps muscles in both groups were stained with cytochrome c oxidase stain (each n=4 × 2). The positive areas of cytochrome c oxidase stain were measured and compared, using computerized densitometry (National Institutes of Health Image program, version 1.61).
  
  
  RESULTS
  In the control group, the lower triceps muscles were not stained with cytochrome c oxidase. In the edaravone group, the lower triceps muscles were strongly stained with cytochrome c oxidase. The positive areas of cytochrome c oxidase stain were significantly greater in the edaravone group (133,000±12,000 μ(2)/mm(2), P<0.01) compared with the control group (8000±1300 μ(2)/mm(2)).
  
  
  CONCLUSIONS
  The present study suggests that the preoperative administration of 9.0 mg/kg of edaravone may suppress postoperative reperfusion injury in a rat model.
authors.affiliation: Department of Cardiovascular Surgery, Hyogo College of Medicine, Hyogo, Japan.
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Yamamura
authors.forename: Yuji
authors.initials: Y
authors.lastname: Miyamoto
authors.forename: Masataka
authors.initials: M
authors.lastname: Mitsuno
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Ohata
authors.forename: Hiroe
authors.initials: H
authors.lastname: Tanaka
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Masaaki
authors.initials: M
authors.lastname: Ryomoto
authors.forename: Yoshiteru
authors.initials: Y
authors.lastname: Yoshioka