title: Magnetic resonance imaging of endothelial cells with vectorized iron oxide nanoparticles.
abstract: We propose a method for obtaining superparamagnetic nanoparticles based on iron oxide and their water suspensions. The structure and size of nanoparticles were confirmed by transmission electron microscopy, dynamic light scattering, and X-ray diffraction analysis. The nanoparticles also contained a fluorescent dye Dil C18. Cytotoxicity of obtained aqueous suspension was studied by MTT assay; low toxicity of nanoparticles was demonstrated. High T2-relaxivity of nanoparticles allows using them as a contrast agent for MRI. After incubation of cerebellar sections with nanoparticles vectorized with antibodies to antigen AMVB1, specific visualization of blood vessels was detected.
authors.affiliation: Department of Medical Nanobiotechnology, Medicobiological Faculty, Russian State Medical University, Ministry of Health Care and Social Development of Russian Federation, Moscow, Russia. abakumov1988@gmail.com
authors.forename: M A
authors.initials: MA
authors.lastname: Abakumov
authors.forename: A E
authors.initials: AE
authors.lastname: Goldt
authors.forename: M
authors.initials: M
authors.lastname: Sokolsky-Papkov
authors.forename: Y A
authors.initials: YA
authors.lastname: Zorkina
authors.forename: V P
authors.initials: VP
authors.lastname: Baklaushev
authors.forename: E A
authors.initials: EA
authors.lastname: Goodilin
authors.forename: A V
authors.initials: AV
authors.lastname: Kabanov
authors.forename: V P
authors.initials: VP
authors.lastname: Chekhonin
mesh_terms: D003287:Contrast Media / Q000737:chemistry; D005290:Ferric Compounds / Q000737:chemistry*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D014961:X-Ray Diffraction
chemical_list: D003287:Contrast Media; D005290:Ferric Compounds; C000499:ferric oxide