title: Association of glutathione S-transferase pi isoform single-nucleotide polymorphisms with exudative age-related macular degeneration in a Chinese population.
abstract: OBJECTIVE
  To investigate the association between single-nucleotide polymorphisms in the pi isoform of glutathione S-transferase (GSTP1) gene and the risk of exudative age-related macular degeneration (AMD) in a Chinese case-control cohort.
  
  
  METHODS
  A total of 131 Chinese patients with exudative AMD and 138 control individuals were recruited. Genomic DNA was extracted from venous blood leukocytes. Two common nonsynonymous single-nucleotide polymorphisms in GSTP1 (rs1695 and rs1138272) were genotyped by polymerase chain reaction followed by allele-specific restriction enzyme digestion and direct sequencing.
  
  
  RESULTS
  Significant association with exudative AMD was detected for single-nucleotide polymorphism, rs1695 (P = 0.019). The risk G allele frequencies were 21.8% in AMD patients and 12.7% in control subjects (P = 0.007). Compared with the wild-type AA genotype, odds ratio for the risk of AMD was 1.91 (95% confidence interval, 1.09-3.35) for the heterozygous AG genotype and 2.52 (95% confidence interval, 0.6-10.61) for the homozygous GG genotype. In contrast, rs1138272 was not associated with exudative AMD (P = 1.00). The risk G allele frequencies of rs1138272 were 0.4% in AMD patients and 0.4% in control subjects (P = 1.00).
  
  
  CONCLUSIONS
  Our data suggest that the GSTP1 variant rs1695 moderately increases the risk of exudative AMD. The variant rs1138272 was rare and was not associated with exudative AMD in this Chinese cohort.
authors.affiliation: Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology and Visual Sciences Key Laboratory, Beijing, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Gu
authors.forename: Erdan
authors.initials: E
authors.lastname: Sun
authors.forename: Lei
authors.initials: L
authors.lastname: Cui
authors.forename: Xiufen
authors.initials: X
authors.lastname: Yang
authors.forename: Apiradee
authors.initials: A
authors.lastname: Lim
authors.forename: Jun
authors.initials: J
authors.lastname: Xu
authors.forename: Torkel
authors.initials: T
authors.lastname: Snellingen
authors.forename: Xipu
authors.initials: X
authors.lastname: Liu
authors.forename: Ningli
authors.initials: N
authors.lastname: Wang
authors.forename: Ningpu
authors.initials: N
authors.lastname: Liu
mesh_terms: D000368:Aged; D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D002681:China; D005122:Exudates and Transudates; D005260:Female; D005451:Fluorescein Angiography; D005838:Genotype; D051549:Glutathione S-Transferase pi / Q000235:genetics*; D006801:Humans; D007527:Isoenzymes / Q000235:genetics; D008297:Male; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D017422:Sequence Analysis, DNA; D057135:Wet Macular Degeneration / Q000175:diagnosis / Q000201:enzymology / Q000235:genetics*
chemical_list: D007527:Isoenzymes; D051549:Glutathione S-Transferase pi