title: Effects of ABO and FUT2 genetic transcription absence on ABH histo-blood group antigen expression in lung cancer patients.
abstract: OBJECTIVE
  To investigate the effect of alterations in mRNAs of ABO and FUT2 genes on the expression of ABH histo-blood group antigens in lung cancer patients.
  
  
  METHODS
  Totals of 18 patients with blood group A, 14 with group B, 8 with group AB and 9 with group O, were assessed for blood group A/B/H antigens by immunohistochemical staining. Expression of A/B enzyme and FUT2 mRNA was detected in tumor tissues and corresponding lung tissues adjacent to tumors from lung cancer patients using RT-PCR.
  
  
  RESULTS
  Expression of FUT2 and A/B enzyme mRNA in lung tissue adjacent to tumors was statistically greater than that in tumor tissues (χ2=14.118, P<0.001). Expression of FUT2 mRNA was statistically lower than that of A/B enzyme in tumor tissues from lung cancer patients whose blood group was A/B/AB (χ2=7.813, P=0.005). Only tumor tissues from 9 patients with mRNA expression of A/B enzyme and FUT2 gene lacked blood group antigens. In particular, expression of A/B antigens was not detected in five cases with A/B mRNA expression, a significant association being observed between the expression of enzyme and antigens (Pearson's R=0.867;kappa's coefficient =0.858, P<0.001).
  
  
  CONCLUSIONS
  Expression of A/B/H blood group antigens was not detected in lung cancer tissues, which may have resulted from down-regulation of ABO/FUT2 gene transcription. Furthermore, the FUT2 gene may indirectly regulate expression of A/B blood group antigens by influencing H antigen expression.
authors.affiliation: Department of Thoracic Surgery, Shandong University, China. lijun2124@sohu.com
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Shuhai
authors.initials: S
authors.lastname: Li
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Zhou
authors.forename: Shuanghu
authors.initials: S
authors.lastname: Yuan
authors.forename: Zhou
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000017:ABO Blood-Group System / Q000378:metabolism*; D000230:Adenocarcinoma / Q000097:blood / Q000235:genetics; D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000097:blood / Q000235:genetics; D005260:Female; D005500:Follow-Up Studies; D005647:Fucosyltransferases / Q000235:genetics*; D006801:Humans; D007124:Immunoenzyme Techniques; D008168:Lung / Q000378:metabolism; D008175:Lung Neoplasms / Q000097:blood* / Q000235:genetics*; D008297:Male; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055752:Small Cell Lung Carcinoma / Q000097:blood / Q000235:genetics; D014158:Transcription, Genetic; D000097631:Galactoside 2-alpha-L-fucosyltransferase
chemical_list: D000017:ABO Blood-Group System; D005647:Fucosyltransferases; D012333:RNA, Messenger; D000097631:Galactoside 2-alpha-L-fucosyltransferase