title: TOUCH-UP gradient amplification method.
abstract: We report a unique amplification technique that works efficiently and specifically over a temperature range, rather than at one specific temperature, throughout the amplification process. As bisulfite-modified DNA is one of the difficult to amplify templates, we used this technique to amplify regions of promoter-associated CpG island for 11 genes using this template. This technique amplified specific products for every gene without requiring any optimization.
authors.affiliation: Brain Tumour UK Neuro-oncology Research Centre, Research Institute in Healthcare Sciences, School of Applied Sciences, University of Wolverhampton, Wolverhampton, United Kingdom. fbrowther@wlv.ac.uk
authors.forename: F B
authors.initials: FB
authors.lastname: Rowther
authors.forename: H
authors.initials: H
authors.lastname: Kardooni
authors.forename: T
authors.initials: T
authors.lastname: Warr
mesh_terms: D045744:Cell Line, Tumor; D018899:CpG Islands; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D019175:DNA Methylation; D056426:Genetic Loci; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA; D013447:Sulfites / Q000737:chemistry
chemical_list: D013447:Sulfites; D004247:DNA