title: Genome editing in induced pluripotent stem cells.
abstract: The discovery of induced pluripotent stem (iPS) cells has broadened the promises of regenerative medicine through the generation of syngeneic replacement cells or tissues via the differentiation of patient-specific iPS cells. To apply iPS cell-mediated therapy to patients with genetic disorders, however, genome-editing technologies with high efficiency and specificity are needed. Recently, several targeted genome-editing strategies mediated by zinc finger nuclease and transcription activator-like effector nuclease have been applied to human and mouse iPS cells. Furthermore, spontaneous homologous recombination can restore genotype to wild type in mouse iPS cells heterozygous for genetic mutations. Through genome editing, the clinical application of patient-specific genetic mutation-free iPS cells to genetic disorders can finally be realized.
authors.affiliation: Stem Cell Engineering, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, Japan.
authors.forename: Li-Tao
authors.initials: LT
authors.lastname: Cheng
authors.forename: Liang-Tso
authors.initials: LT
authors.lastname: Sun
authors.forename: Takashi
authors.initials: T
authors.lastname: Tada
mesh_terms: D000818:Animals; D002460:Cell Line; D053903:DNA Breaks, Double-Stranded; D004260:DNA Repair; D003851:Deoxyribonucleases / Q000737:chemistry / Q000378:metabolism; D016678:Genome; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000378:metabolism*; D051379:Mice; D033581:Stem Cell Transplantation; D015533:Transcriptional Activation; D016335:Zinc Fingers
chemical_list: D003851:Deoxyribonucleases