title: Effect of some pyrimidine compounds on rat brain monoamine oxidase-B in vitro.
abstract: The effects of some pyrimidine compounds (PCs) including barbituric acid (BA) 5,5-diethyl barbituric acid (DEBA), 2-thiobarbituric acid (TBA), violuric acid (VA), 2-thiouracil (TU), and 6-amino-2-thiouracil (ATU) on the activity of rat brain monoamine oxidase-B (MAO-B) were investigated. The results revealed that MAO-B was activated by BA, DEBA, TBA, TU, and ATU, and the activation was structural, concentration, and time dependent. However, MAO-B was inhibited by VA in a noncompetitive and irreversible manner with an enzyme-inhibitor dissociation constant (K (i) value) of 32 nM and IC(50) equals to 19 nM. All the studied PCs changed both the optimum pH and temperature of MAO-B.
authors.affiliation: Biochemistry Department, Faculty of Science, Alexandria University, Alexandria, Egypt. nshaban2001@yahoo.co.uk
authors.forename: Nadia Z
authors.initials: NZ
authors.lastname: Shaban
authors.forename: Mamdouh S
authors.initials: MS
authors.lastname: Masoud
authors.forename: Mai A
authors.initials: MA
authors.lastname: Mawlawi
authors.forename: Doaa
authors.initials: D
authors.lastname: Awad
authors.forename: Omayma M
authors.initials: OM
authors.lastname: Sadek
mesh_terms: D000818:Animals; D001463:Barbiturates / Q000494:pharmacology*; D001596:Benzylamines / Q000378:metabolism; D001921:Brain / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008297:Male; D008928:Mitochondria / Q000187:drug effects / Q000201:enzymology; D008995:Monoamine Oxidase / Q000378:metabolism*; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology*; D010084:Oxidation-Reduction; D011743:Pyrimidines / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D001463:Barbiturates; D001596:Benzylamines; D008996:Monoamine Oxidase Inhibitors; D011743:Pyrimidines; C094277:violuric acid; C030796:benzylamine; D008995:Monoamine Oxidase