title: Mesofluidic devices for DNA-programmed combinatorial chemistry.
abstract: Hybrid combinatorial chemistry strategies that use DNA as an information-carrying medium are proving to be powerful tools for molecular discovery. In order to extend these efforts, we present a highly parallel format for DNA-programmed chemical library synthesis. The new format uses a standard microwell plate footprint and is compatible with commercially available automation technology. It can accommodate a wide variety of combinatorial synthetic schemes with up to 384 different building blocks per chemical step. We demonstrate that fluidic routing of DNA populations in the highly parallel format occurs with excellent specificity, and that chemistry on DNA arrayed into 384 well plates proceeds robustly, two requirements for the high-fidelity translation and efficient in vitro evolution of small molecules.
authors.affiliation: Department of Chemistry, Stanford University, Stanford, California, United States of America.
authors.forename: Rebecca M
authors.initials: RM
authors.lastname: Weisinger
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Marinelli
authors.forename: S Jarrett
authors.initials: SJ
authors.lastname: Wrenn
authors.forename: Pehr B
authors.initials: PB
authors.lastname: Harbury
mesh_terms: D015139:Blotting, Southern; D020650:Combinatorial Chemistry Techniques / Q000295:instrumentation* / Q000379:methods*; D004247:DNA / Q000235:genetics*; D015723:Gene Library; D009693:Nucleic Acid Hybridization; D015203:Reproducibility of Results; D054852:Small Molecule Libraries
chemical_list: D054852:Small Molecule Libraries; D004247:DNA