title: New approaches to molecular diagnostics of prenatal pathology.
abstract: Analysis of the spectrum of amniotic fluid proteins in physiological and abnormal pregnancy using proteomic analysis allowed detection of a number of difference proteins, that are absent or, alternatively, appear in gestosis. Among absent proteins, there were NADPH-dependent carbonyl reductase, epidermal fatty acid-binding protein, haptoglobin, calgranulins A and B. In contrast to proteomic spectrum of amniotic fluid in physiological pregnancy, 7 new proteins appear during gestosis, 3 of them were identified: C area of immunoglobulin K-chain, breast cancer metastasis suppressor-1, and protein-1 containing AIG2-like domain. Possible effects of revealed differences in proteomic spectrum on development of main disturbances during gestosis are discussed. Difference proteins detected in amniotic fluid may serve as gestosis markers.
authors.affiliation: Rostov Institute of Obstetrics and Pediatrics, Federal Agency for High-Technological Medical Care, Rostov-on-Don, Russia. rniiap@yandex.ru
authors.forename: T N
authors.initials: TN
authors.lastname: Pogorelova
authors.forename: V I
authors.initials: VI
authors.lastname: Orlov
authors.forename: V O
authors.initials: VO
authors.lastname: Gunko
mesh_terms: D000328:Adult; D000653:Amniotic Fluid / Q000378:metabolism; D005260:Female; D006801:Humans; D011247:Pregnancy; D011248:Pregnancy Complications / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D011257:Pregnancy Proteins / Q000378:metabolism; D011296:Prenatal Diagnosis / Q000379:methods*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D055815:Young Adult
chemical_list: D011257:Pregnancy Proteins; D020543:Proteome