title: A highly sensitive aptasensor towards Plasmodium lactate dehydrogenase for the diagnosis of malaria.
abstract: Finding a highly sensitive diagnostic technique for malaria has challenged scientists for the last century. In the present study, we identified versatile single-strand DNA aptamers for Plasmodium lactate dehydrogenase (pLDH), a biomarker for malaria, via the Systematic Evolution of Ligands by EXponential enrichment (SELEX). The pLDH aptamers selectively bound to the target proteins with high sensitivity (K(d)=16.8-49.6 nM). The selected aptamers were characterized using an electrophoretic mobility shift assay, a quartz crystal microbalance, a fluorescence assay, and circular dichroism spectroscopy. We also designed a simple aptasensor using electrochemical impedance spectroscopy; both Plasmodium vivax LDH and Plasmodium falciparum LDH were selectively detected with a detection limit of 1 pM. Furthermore, the pLDH aptasensor clearly distinguished between malaria-positive blood samples of two major species (P. vivax and P. falciparum) and a negative control, indicating that it may be a useful tool for the diagnosis, monitoring, and surveillance of malaria.
authors.affiliation: Department of Chemistry, Pohang University of Science and Technology, Pohang, Gyeongbuk, 790-784, Republic of Korea.
authors.forename: Seonghwan
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Chemistry, Pohang University of Science and Technology, Pohang, Gyeongbuk, 790-784, Republic of Korea.
authors.forename: Kyung-Mi
authors.initials: KM
authors.lastname: Song
authors.affiliation: Department of Chemistry, Pohang University of Science and Technology, Pohang, Gyeongbuk, 790-784, Republic of Korea.
authors.forename: Weejeong
authors.initials: W
authors.lastname: Jeon
authors.affiliation: Department of Chemistry, Pohang University of Science and Technology, Pohang, Gyeongbuk, 790-784, Republic of Korea.
authors.forename: Hunho
authors.initials: H
authors.lastname: Jo
authors.affiliation: Department of Chemistry, Pusan National University, Busan, 609-735, Republic of Korea.
authors.forename: Yoon-Bo
authors.initials: YB
authors.lastname: Shim
authors.affiliation: Department of Chemistry, Pohang University of Science and Technology, Pohang, Gyeongbuk, 790-784, Republic of Korea. Electronic address: ciban@postech.ac.kr.
authors.forename: Changill
authors.initials: C
authors.lastname: Ban
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry; D001483:Base Sequence; D015415:Biomarkers / Q000097:blood; D015374:Biosensing Techniques / Q000379:methods* / Q000706:statistics & numerical data; D002942:Circular Dichroism; D058266:Dielectric Spectroscopy; D024202:Electrophoretic Mobility Shift Assay; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000097:blood*; D057230:Limit of Detection; D008288:Malaria / Q000175:diagnosis* / Q000201:enzymology / Q000469:parasitology; D016778:Malaria, Falciparum / Q000175:diagnosis; D016780:Malaria, Vivax / Q000175:diagnosis; D009690:Nucleic Acid Conformation; D010961:Plasmodium / Q000201:enzymology*; D058525:Quartz Crystal Microbalance Techniques; D052156:SELEX Aptamer Technique / Q000379:methods* / Q000706:statistics & numerical data
chemical_list: D052157:Aptamers, Nucleotide; D015415:Biomarkers; D007770:L-Lactate Dehydrogenase