title: Antioxidant activity by DPPH assay of potential solutions to be applied on bleached teeth.
abstract: The aim of this study was to assess, using the DPPH assay, the antioxidant activity of several substances that could be proposed to immediately revert the problems caused by bleaching procedures. The percentage of antioxidant activity (AA%) of 10% ascorbic acid solution (AAcidS), 10% ascorbic acid gel (AAcidG), 10% sodium ascorbate solution (SodAsS), 10% sodium ascorbate gel (SodAsG), 10% sodium bicarbonate (Bicarb), Neutralize(®) (NE), Desensibilize(®) (DES), catalase C-40 at 10 mg/mL (CAT), 10% alcohol solution of alpha-tocopherol (VitE), Listerine(®) (LIS), 0.12% chlorhexidine (CHX), Croton Lechleri (CL), 10 % aqueous solution of Uncaria Tomentosa (UT), artificial saliva (ArtS) and 0.05% sodium fluoride (NaF) was assessed in triplicate by 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) free radical assay. All substances exhibited antioxidant activity, except for CL. AAcidS, AAcidG and VitE exhibited the highest AA% (p<0.05). On the contrary, CHX, NE, LIS and NaF showed the lowest AA% (p<0.05). In conclusion, AAcidS, AAcidG, SodAsS, SodAsG and VitE presented the highest antioxidant activity among substances tested in this study. The DPPH assay provides an easy and rapid way to evaluate potential antioxidants.
authors.affiliation: Department of Dental Materials, Dental School, University of São Paulo, Brazil.
authors.forename: Eugenio José
authors.initials: EJ
authors.lastname: Garcia
authors.forename: Tatiane Luiza Cadorin
authors.initials: TL
authors.lastname: Oldoni
authors.forename: Severino Matias de
authors.initials: SM
authors.lastname: Alencar
authors.forename: Alessandra
authors.initials: A
authors.lastname: Reis
authors.forename: Alessandro D
authors.initials: AD
authors.lastname: Loguercio
authors.forename: Rosa Helena Miranda
authors.initials: RH
authors.lastname: Grande
mesh_terms: D000704:Analysis of Variance; D000975:Antioxidants / Q000737:chemistry*; D001713:Biphenyl Compounds / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry*; D006801:Humans; D007202:Indicators and Reagents; D010851:Picrates / Q000737:chemistry*; D012996:Solutions / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D014073:Tooth Bleaching / Q000009:adverse effects*
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D007202:Indicators and Reagents; D010851:Picrates; D012996:Solutions; C004931:1,1-diphenyl-2-picrylhydrazyl