title: Development and characterization of microsatellite markers for the wild South American Passiflora cincinnata (Passifloraceae).
abstract: OBJECTIVE
  We developed the first set of microsatellite markers for the passion fruit, Passiflora cincinnata, to provide tools for further study of its genetic diversity and to support current conservation and genetic studies.
  
  
  RESULTS
  We used a microsatellite-enriched library approach to isolate and characterize 25 new molecular markers. Seven primer pairs successfully amplified polymorphic microsatellite regions, with an average of five alleles per locus. The mean values of expected and observed levels of heterozygosity were 0.516 and 0.525, respectively.
  
  
  CONCLUSIONS
  The microsatellite markers identified in this study may be valuable tools for population genetic studies, and this set of markers also may be useful in the design of a genetic conservation strategy, mating system, and hybridization studies.
authors.affiliation: Centro de Biologia Molecular e Engenharia Genética and Instituto de Biologia, Universidade Estadual de Campinas, Campinas, São Paulo, Brazil.
authors.forename: Carlos B M
authors.initials: CB
authors.lastname: Cerqueira-Silva
authors.forename: Elisa S L
authors.initials: ES
authors.lastname: Santos
authors.forename: Alisson M
authors.initials: AM
authors.lastname: Souza
authors.forename: Gustavo M
authors.initials: GM
authors.lastname: Mori
authors.forename: Eder J
authors.initials: EJ
authors.lastname: Oliveira
authors.forename: Ronan X
authors.initials: RX
authors.lastname: Corrêa
authors.forename: Anete P
authors.initials: AP
authors.lastname: Souza
mesh_terms: D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D029598:Passiflora / Q000235:genetics*; D011110:Polymorphism, Genetic; D013020:South America