title: A novel sorptive extraction method based on polydimethylsiloxane frit for determination of lung cancer biomarkers in human serum.
abstract: In this study, a porous polypropylene frit was coated with polydimethylsiloxane (PDMS) as extraction medium, based on the home-made PDMS-frit, a rapid, simple and sensitive sorptive extraction method was established for analysis of potential biomarkers of lung cancer (hexanal and heptanal) in human serum samples. In the method, derivatization and extraction occurred simultaneously on the PDMS-frit, then the loaded frit was ultrasonically desorbed in acetonitrile. Polymerization, derivatization-extraction and desorption conditions were optimized. Under the optimal conditions, satisfactory results were gained, a wide linear application range was obtained in the range of 0.002-5.0 μmol L(-1) (R>0.997) for two aldehydes, the detection limits (SN(-1)=3) were 0.5 nmol L(-1) for hexanal and 0.4 nmol L(-1) for heptanal. The relative standard deviations (RSDs, n=5) of the method were below 7.9% and the recoveries were above 72.7% for the spiked serum. All these results hint that the proposed method is potential for disease markers analysis in complex biological samples.
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, Wuhan, China. huixu@mail.ccnu.edu.cn
authors.forename: Hui
authors.initials: H
authors.lastname: Xu
authors.forename: Shuyu
authors.initials: S
authors.lastname: Wang
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D000327:Adsorption; D000447:Aldehydes / Q000097:blood*; D014408:Biomarkers, Tumor / Q000097:blood*; D016022:Case-Control Studies; D002851:Chromatography, High Pressure Liquid; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D008175:Lung Neoplasms / Q000097:blood*; D011126:Polypropylenes / Q000737:chemistry; D016062:Porosity; D052617:Solid Phase Microextraction / Q000379:methods*; D013010:Sonication
chemical_list: D000097:Acetonitriles; D000447:Aldehydes; D014408:Biomarkers, Tumor; D004129:Dimethylpolysiloxanes; D011126:Polypropylenes; C046204:heptanal; C010463:n-hexanal; C032159:acetonitrile