title: The emerging issue of human resident arterial progenitors: the contribution of organ culture.
abstract: Human femoral arteries were cultured up to 56 days. Samples were processed for light, immunohistochemical, and transmission electron microscopy. Arteries became rapidly depopulated; at day 42, an endothelial lining (CD31(+), Weibel-Palade bodies) developed on the intima; endothelium was in continuity with mesenchymal stromal cells (CD44(+), CD90(low), CD105(low)) placed on adventitia. The media-adventitia area showed heterogeneous cell populations. In long-term organ culture, femoral artery develops a continuous cell coverage that differentiates to endothelium on the intima exclusively. This suggests that distinct topographical factors, such as resident progenitors and/or matrix signals, are able to regulate vascular homeostasis in adult life.
authors.affiliation: Department of Specialistic Surgical and Anaesthesiological Sciences, S. Orsola Hospital, University of Bologna, Bologna, Italy. sab.valente@gmail.com
authors.forename: S
authors.initials: S
authors.lastname: Valente
authors.forename: N
authors.initials: N
authors.lastname: Panarese
authors.forename: M
authors.initials: M
authors.lastname: Buzzi
authors.forename: M
authors.initials: M
authors.lastname: Alberghini
authors.forename: A
authors.initials: A
authors.lastname: Stella
authors.forename: G
authors.initials: G
authors.lastname: Pasquinelli
mesh_terms: D001158:Arteries / Q000378:metabolism / Q000648:ultrastructure*; D042783:Endothelial Cells / Q000378:metabolism / Q000648:ultrastructure; D004730:Endothelium, Vascular / Q000378:metabolism / Q000648:ultrastructure*; D005109:Extracellular Matrix / Q000378:metabolism; D005263:Femoral Artery / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D046529:Microscopy, Electron, Transmission; D032389:Myocytes, Smooth Muscle / Q000378:metabolism / Q000648:ultrastructure; D009924:Organ Culture Techniques; D013234:Stem Cells / Q000378:metabolism / Q000648:ultrastructure*; D014746:Vimentin / Q000378:metabolism
chemical_list: D014746:Vimentin