title: Detachment strength of human osteoblasts cultured on hydroxyapatite with various surface roughness. Contribution of integrin subunits.
abstract: Hydroxyapatite (HA) has been widely used as a bone substitute in dental, maxillofacial and orthopaedic surgery and as osteoconductive bone substitute or precoating of pedicle screws and cages in spine surgery. The aim of the present study was to investigate the osteoblastic adhesion strength on HA substrata with different surface topography and biochemistry (pre-adsorption of fibronectin) after blocking of specific integrin subunits with monoclonal antibodies. Stoichiometric HA was prepared by precipitation followed by ageing and characterized by SEM, EDX, powder XRD, Raman spectroscopy, TGA, and specific surface area analysis. Human bone marrow derived osteoblasts were cultured on HA disc-shaped substrata which were sintered and polished resulting in two surface roughness grades. For attachment evaluation, cells were incubated with monoclonal antibodies and seeded for 2 h on the substrata. Cell detachment strength was determined using a rotating disc device. Cell detachment strength was surface roughness, fibronectin preadsorption and intergin subunit sensitive.
authors.affiliation: Department of Mechanical Engineering and Aeronautics, University of Patras, Rion, Patras, Greece.
authors.forename: Petros A
authors.initials: PA
authors.lastname: Kokkinos
authors.forename: Petros G
authors.initials: PG
authors.lastname: Koutsoukos
authors.forename: Despina D
authors.initials: DD
authors.lastname: Deligianni
mesh_terms: D019138:Antibodies, Blocking; D000911:Antibodies, Monoclonal; D001672:Biocompatible Materials / Q000737:chemistry*; D001696:Biomechanical Phenomena; D018786:Bone Substitutes / Q000737:chemistry; D002448:Cell Adhesion / Q000502:physiology*; D018929:Cell Culture Techniques; D048430:Cell Shape / Q000502:physiology; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible; D017886:Durapatite / Q000737:chemistry*; D005353:Fibronectins; D006801:Humans; D039564:Integrin alphaV / Q000502:physiology; D019012:Integrin beta1 / Q000502:physiology; D039661:Integrin beta3; D016023:Integrins / Q000037:antagonists & inhibitors / Q000502:physiology*; D008422:Materials Testing / Q000295:instrumentation; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D013314:Stress, Mechanical; D013499:Surface Properties
chemical_list: D019138:Antibodies, Blocking; D000911:Antibodies, Monoclonal; D001672:Biocompatible Materials; D018786:Bone Substitutes; D020099:Coated Materials, Biocompatible; D005353:Fibronectins; D039564:Integrin alphaV; D019012:Integrin beta1; D039661:Integrin beta3; D016023:Integrins; D017886:Durapatite