title: miR-15a and 16-1 are downregulated in CD4+ T cells of multiple sclerosis relapsing patients.
abstract: The pathology of relapsing-remitting multiple sclerosis (RR-MS) is largely attributed to activated autoreactive effector T lymphocytes. The influence of microRNAs on the immune response has been shown to occur in different pathways of lymphocyte differentiation and function. Here, the expression of the miRNAs miR-15a/16-1 in PBMC, CD4(+), and CD8(+) from RR-MS patients has been investigated. BCL2, a known miR-15a/16-1 target, has also been analyzed. The results have shown that miR-15a/16-1 is downregulated in CD4(+) T cells, whereas BCL2 is highly expressed in RR-MS patients only. Our data suggest that miR-15a/16-1 can also modulate the BCL2 gene expression in CD4(+) T cells from RR-MS patients, thereby affecting apoptosis processes.
authors.affiliation: Genetics Department, São Paulo University Medical school of Ribeirão Preto, Ribeirão Preto, Brazil.
authors.forename: Julio Cesar Cetrulo
authors.initials: JC
authors.lastname: Lorenzi
authors.forename: Doralina G
authors.initials: DG
authors.lastname: Brum
authors.forename: Dalila L
authors.initials: DL
authors.lastname: Zanette
authors.forename: Alessandra
authors.initials: A
authors.lastname: de Paula Alves Souza
authors.forename: Fernanda Gonçalves
authors.initials: FG
authors.lastname: Barbuzano
authors.forename: Antonio Carlos
authors.initials: AC
authors.lastname: Dos Santos
authors.forename: Amilton Antunes
authors.initials: AA
authors.lastname: Barreira
authors.forename: Wilson Araujo
authors.initials: WA
authors.lastname: da Silva
mesh_terms: D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism*; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism; D016022:Case-Control Studies; D049109:Cell Proliferation; D004185:Disability Evaluation; D015536:Down-Regulation / Q000276:immunology*; D005260:Female; D006801:Humans; D008213:Lymphocyte Activation / Q000276:immunology; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D055815:Young Adult
chemical_list: C505361:MIRN15 microRNA, human; C505362:MIRN16 microRNA, human; D035683:MicroRNAs; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger