title: Preparative 2D Gel Electrophoresis with Immobilized pH Gradients: SDS-PAGE of Proteins.
abstract: INTRODUCTIONFollowing first-dimension IEF and equilibration of the IPG gel strips, the proteins are separated on the basis of their molecular weight in the second dimension on an SDS-PAGE gel. Systems for this separation are available from a variety of suppliers and are commonly found in many protein chemistry laboratories. This protocol describes a method for placement of the IPG strip and gives some recommended electrophoresis conditions for these second-dimension gels.
authors.forename: Wayne R
authors.initials: WR
authors.lastname: Stochaj
authors.forename: Tom
authors.initials: T
authors.lastname: Berkelman
authors.forename: Nancy
authors.initials: N
authors.lastname: Laird