title: Enhanced α-ketoglutaric acid production in Yarrowia lipolytica WSH-Z06 by an improved integrated fed-batch strategy.
abstract: This study aimed at enhancing α-ketoglutaric acid (α-KG) production by Yarrowia lipolytica WSH-Z06. Batch culture experiments demonstrated that CaCO(3) and a relatively low pH (3.0) in the α-KG production phase contributed to α-KG synthesis. Using a two-stage pH control strategy, in which pH was buffered by CaCO(3) in the growth phase and then maintained at 3.0 in the α-KG production phase, the yield of α-KG reached 53.4 g L(-1). In the later phase of batch fermentation, the glycerol was exhausted but synthesis of α-KG did not cease. Therefore, glycerol was fed with an integrated fed-batch mode, and α-KG production increased to 66.2 g L(-1) with a productivity of 0.35 g L(-1) h(-1). Compared to optimal batch culture, α-KG production and productivity were enhanced by 23.9% and 16.7%, respectively. The two-stage pH control strategy, constant feeding approach and lower pH in later phase would be useful for α-KG industrial production.
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China.
authors.forename: Zongzhong
authors.initials: Z
authors.lastname: Yu
authors.forename: Guocheng
authors.initials: G
authors.lastname: Du
authors.forename: Jingwen
authors.initials: J
authors.lastname: Zhou
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
mesh_terms: D061249:Batch Cell Culture Techniques / Q000295:instrumentation*; D019149:Bioreactors / Q000382:microbiology*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007656:Ketoglutaric Acids / Q000302:isolation & purification* / Q000378:metabolism*; D018511:Systems Integration; D025062:Yarrowia / Q000378:metabolism*
chemical_list: D007656:Ketoglutaric Acids