title: Differential phase-contrast, swept-source optical coherence tomography at 1060 nm for in vivo human retinal and choroidal vasculature visualization.
abstract: Human retinal and choroidal vasculature was visualized by a differential phase-contrast (DPC) method using high-speed, swept-source optical coherence tomography (SS-OCT) at 1060 nm. The vasculature was recognized as regions of motion by creating differential phase-variance (DPV) tomograms: multiple B-scans of individual slices through the retina were collected and the variance of the phase differences was calculated. DPV captured the small vessels and the meshwork of capillaries associated with the inner retina in en-face images over 4 mm(2). The swept-source laser at 1060 nm offered the needed phase sensitivity to perform DPV and generated en-face images that capture motion in the inner choroidal layer exceeding the capabilities of previous spectrometer-based instruments. In comparison with the power Doppler phase-shift method, DPV provided better visualization of the foveal avascular zone in en-face images.
authors.affiliation: California Institute of Technology, Beckman Institute, Division of Biology, Pasadena, California 91125, USA. rmotaghi@caltech.edu
authors.forename: S M Reza
authors.initials: SM
authors.lastname: Motaghiannezam
authors.forename: David
authors.initials: D
authors.lastname: Koos
authors.forename: Scott E
authors.initials: SE
authors.lastname: Fraser
mesh_terms: D000792:Angiography / Q000295:instrumentation*; D002829:Choroid / Q000098:blood supply*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D018613:Microscopy, Confocal / Q000295:instrumentation*; D008858:Microscopy, Phase-Contrast / Q000295:instrumentation*; D015203:Reproducibility of Results; D012171:Retinal Vessels / Q000033:anatomy & histology*; D042261:Retinoscopes; D012680:Sensitivity and Specificity; D041623:Tomography, Optical Coherence / Q000295:instrumentation*