title: Antibiotic resistance of Escherichia coli isolated from a poultry slaughterhouse.
abstract: The aim of the study was to investigate the antibiotic resistant E. coli strains isolated from bioaerosols and surface swabs in a slaughterhouse as a possible source of poultry meat contamination. The highest air coliforms contamination was during shackling, killing and evisceration of poultry. The strains showed resistance to ampicillin (89%), ceftiofur (62%) and cefquinome (22%), while resistance to ampicillin with sulbactam was only 6%. Resistance to streptomycin and gentamicin was detected in 43% vs. 14% isolates; to tetracycline 33%; to chloramphenicol and florfenicol in 10% vs. 18% isolates; to cotrimoxazol in 35% isolates; to enrofloxacin in 43% isolates. The higher MIC of ceftazidime (3.6 mg x l(-1)) and ceftriaxon (5.2 mg x l(-1)) revealed the presence of ESBLs in 43% of isolates. From 19 selected phenotypically ESBL positive strains, 16 consisted of CMY-2 genes, while CTX-M genes were not detected by PCR. Maldi tof analysis of selected E. coli showed a clear clonal relatedness of environmental strains from various withdrawals.
authors.affiliation: University of Veterinary Medicine and Pharmacy, Kosice, Slovakia.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Gregova
authors.forename: Marta
authors.initials: M
authors.lastname: Kmetova
authors.forename: Vladimfr
authors.initials: V
authors.lastname: Kmet
authors.forename: Jan
authors.initials: J
authors.lastname: Venglovsky
authors.forename: Alexander
authors.initials: A
authors.lastname: Feher
mesh_terms: D000003:Abattoirs; D000391:Air Microbiology; D016902:Air Pollution, Indoor / Q000032:analysis; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015169:Colony Count, Microbial; D024881:Drug Resistance, Bacterial; D004755:Enterobacteriaceae / Q000145:classification / Q000302:isolation & purification; D004926:Escherichia coli / Q000145:classification / Q000187:drug effects* / Q000302:isolation & purification; D005516:Food Microbiology; D008460:Meat / Q000382:microbiology*; D008826:Microbial Sensitivity Tests / Q000662:veterinary; D011200:Poultry / Q000382:microbiology*; D018154:Slovakia; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000662:veterinary
chemical_list: D000900:Anti-Bacterial Agents