title: An amperometric biosensor for choline determination prepared from choline oxidase immobilized in polypyrrole-polyvinylsulfonate film.
abstract: In this paper, a novel amperometric choline biosensor with immobilization of choline oxidase on electrochemically polymerized polypyrrole-polyvinylsulphonate (PPy-PVS) film has been accomplished via the entrapment technique. The effects of pH and temperature were investigated and optimum parameters were found to be 9.0 and 60 °C, respectively. There are two linear parts in the region between 1.0 × 10 (-7) - 1.0 × 10 (-6)M (R(2) = 0.997) and 1.0 × 10 (-5) - 1.0 × 10 (-3) M (R(2) = 0.986). The storage stability and operation stability of the enzyme electrode were also studied.
authors.affiliation: Department of Chemistry, Faculty of Arts and Sciences, Gazi University, Ankara, Turkey.
authors.forename: Merve
authors.initials: M
authors.lastname: Özdemir
authors.forename: Fatma
authors.initials: F
authors.lastname: Arslan
authors.forename: Halit
authors.initials: H
authors.lastname: Arslan
mesh_terms: D000421:Alcaligenes / Q000201:enzymology; D000429:Alcohol Oxidoreductases / Q000737:chemistry* / Q000378:metabolism; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002138:Calibration; D002794:Choline / Q000032:analysis* / Q000737:chemistry; D004563:Electrochemistry; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D011108:Polymers / Q000737:chemistry*; D011145:Polyvinyls / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D013451:Sulfonic Acids / Q000737:chemistry*; D013696:Temperature
chemical_list: D004800:Enzymes, Immobilized; D011108:Polymers; D011145:Polyvinyls; D011758:Pyrroles; D013451:Sulfonic Acids; C008959:lyapolate; C067635:polypyrrole; D000429:Alcohol Oxidoreductases; C019888:choline oxidase; D002794:Choline