title: Parallel and patterned optogenetic manipulation of neurons in the brain slice using a DMD-based projector.
abstract: Optical manipulation technologies greatly advanced the understanding of the neuronal network and its dysfunctions. To achieve patterned and parallel optical switching, we developed a microscopic illumination system using a commercial DMD-based projector and a software program. The spatiotemporal patterning of the system was evaluated using acute slices of the hippocampus. The neural activity was optically manipulated, positively by the combination of channelrhodopsin-2 (ChR2) and blue light, and negatively by the combination of archaerhodopsin-T (ArchT) and green light. It is suggested that our projector-managing optical system (PMOS) would effectively facilitate the optogenetic analyses of neurons and their circuits.
authors.affiliation: Department of Developmental Biology and Neuroscience, Tohoku University Graduate School of Life Sciences, Sendai 980-8577, Japan.
authors.forename: Seiichiro
authors.initials: S
authors.lastname: Sakai
authors.forename: Kenichi
authors.initials: K
authors.lastname: Ueno
authors.forename: Toru
authors.initials: T
authors.lastname: Ishizuka
authors.forename: Hiromu
authors.initials: H
authors.lastname: Yawo
mesh_terms: D000818:Animals; D001921:Brain / Q000502:physiology; D057809:HEK293 Cells; D006801:Humans; D008853:Microscopy / Q000295:instrumentation / Q000379:methods; D009474:Neurons / Q000502:physiology*; D055096:Optical Devices; D062308:Optogenetics / Q000295:instrumentation* / Q000379:methods; D009924:Organ Culture Techniques; D018408:Patch-Clamp Techniques; D010775:Photic Stimulation / Q000295:instrumentation* / Q000379:methods; D051381:Rats; D012984:Software