title: MiR-483-5p suppresses the proliferation of glioma cells via directly targeting ERK1.
abstract: MicroRNAs (miRNAs) exhibit tumor-specific expression signatures and play crucial roles in tumorigenesis by targeting oncogenes. Here, through analyzing the miRNA-array profiles of human glioblastoma tissues and the adjacent normal brain tissues, we found miR-483-5p was significantly down-regulated in gliomas, which was confirmed in both human glioma specimens and cell lines. The overexpression of miR-483-5p suppressed glioma cell proliferation and induced a G0/G1 arrest. In contrast, miR-483-5p inhibition promoted cell proliferation. Furthermore, by a dual-luciferase reporter assay and expression analysis, we identified extracellular signal-regulated kinase 1 (ERK1) as a direct target of miR-483-5p. ERK1 knockdown can block cell proliferation induced by miR-483-5p inhibition. Thus, our findings provide the first evidence that miR-483-5p can serve as a tumor suppressor in gliomas.
authors.affiliation: Department of Neurology, Xijing Hospital, The Fourth Military Medical University, Xi'an, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Ming
authors.initials: M
authors.lastname: Shi
authors.forename: Shuangxing
authors.initials: S
authors.lastname: Hou
authors.forename: Bojun
authors.initials: B
authors.lastname: Ding
authors.forename: Lijuan
authors.initials: L
authors.lastname: Liu
authors.forename: Xituan
authors.initials: X
authors.lastname: Ji
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Yanchun
authors.initials: Y
authors.lastname: Deng
mesh_terms: D001483:Base Sequence; D001921:Brain / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation / Q000235:genetics; D059585:G1 Phase Cell Cycle Checkpoints / Q000235:genetics; D005910:Glioma / Q000235:genetics / Q000473:pathology*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D048052:Mitogen-Activated Protein Kinase 3 / Q000235:genetics*
chemical_list: C548912:MIRN483 microRNA, human; D035683:MicroRNAs; D048052:Mitogen-Activated Protein Kinase 3