title: Determination of dopamine in pharmaceutical formulation using enhanced luminescence from europium complex.
abstract: Biologically important compound dopamine plays an important role in the central and peripheral nervous systems. Insufficient dopamine level due to the loss of dopamine producing cells may lead to disease called Schizophrenia and Parkinson's disease. Hence, a simple and fast detection of dopamine is necessary to study in the fields of neurophysiology and clinical medicine. An enhanced fluorimetric determination of dopamine in the presence of ascorbic acid is achieved using photoluminescence of europium complex, Eu(III)-dipicolinic acid. In order to obtain better responses, several operational parameters have been investigated. Under the optimum conditions, the method showed good stability and reproducibility. The application of this method for the determination of dopamine neurotransmitters was satisfactory. Linear response was found down to 3.0 × 10(-7)M with limit of detection 1.0 × 10(-8)M. The relative standard deviation was found to be 3.33% from 20 independent measurements for 1.0 × 10(-5)M of dopamine.
authors.affiliation: Advanced Materials Research Chair, Department of Chemistry, College of Science, King Saud University, Riyadh, Saudi Arabia. tarabai22@yahoo.com.sg
authors.forename: Saikh Mohammad
authors.initials: SM
authors.lastname: Wabaidur
authors.forename: Zeid Abdullah
authors.initials: ZA
authors.lastname: Alothman
authors.forename: Mu
authors.initials: M
authors.lastname: Naushad
mesh_terms: D001205:Ascorbic Acid / Q000737:chemistry; D056831:Coordination Complexes / Q000737:chemistry; D004298:Dopamine / Q000032:analysis*; D015259:Dopamine Agents / Q000032:analysis*; D005063:Europium / Q000737:chemistry*; D057230:Limit of Detection; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D010848:Picolinic Acids / Q000737:chemistry; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D056831:Coordination Complexes; D015259:Dopamine Agents; D004364:Pharmaceutical Preparations; D010848:Picolinic Acids; D005063:Europium; D001205:Ascorbic Acid; C004860:dipicolinic acid; D004298:Dopamine