title: Protection against SHIV-KB9 infection by combining rDNA and rFPV vaccines based on HIV multiepitope and p24 protein in Chinese rhesus macaques.
abstract: Developing an effective vaccine against HIV infection remains an urgent goal. We used a DNA prime/fowlpox virus boost regimen to immunize Chinese rhesus macaques. The animals were challenged intramuscularly with pathogenic molecularly cloned SHIV-KB9. Immunogenicity and protective efficacy of vaccines were investigated by measuring IFN-γ levels, monitoring HIV-specific binding antibodies, examining viral load, and analyzing CD4/CD8 ratio. Results show that, upon challenge, the vaccine group can induce a strong immune response in the body, represented by increased expression of IFN-γ, slow and steady elevated antibody production, reduced peak value of acute viral load, and increase in the average CD4/CD8 ratio. The current research suggests that rapid reaction speed, appropriate response strength, and long-lasting immune response time may be key protection factors for AIDS vaccine. The present study contributes significantly to AIDS vaccine and preclinical research.
authors.affiliation: Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, LiuYingXi Road No. 666, Changchun 130122, China.
authors.forename: Chang
authors.initials: C
authors.lastname: Li
authors.forename: Zhenwei
authors.initials: Z
authors.lastname: Shen
authors.forename: Xiao
authors.initials: X
authors.lastname: Li
authors.forename: Jieying
authors.initials: J
authors.lastname: Bai
authors.forename: Lin
authors.initials: L
authors.lastname: Zeng
authors.forename: Mingyao
authors.initials: M
authors.lastname: Tian
authors.forename: Ying Jin
authors.initials: YJ
authors.lastname: Song
authors.forename: Ming
authors.initials: M
authors.lastname: Ye
authors.forename: Shouwen
authors.initials: S
authors.lastname: Du
authors.forename: Dayong
authors.initials: D
authors.lastname: Ren
authors.forename: Cunxia
authors.initials: C
authors.lastname: Liu
authors.forename: Na
authors.initials: N
authors.lastname: Zhu
authors.forename: Dandan
authors.initials: D
authors.lastname: Sun
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.forename: Ningyi
authors.initials: N
authors.lastname: Jin
mesh_terms: D016915:AIDS Vaccines / Q000008:administration & dosage* / Q000235:genetics; D000818:Animals; D016516:CD4-CD8 Ratio; D002681:China; D004279:DNA, Viral / Q000235:genetics / Q000276:immunology*; D005260:Female; D005587:Fowlpox virus / Q000235:genetics / Q000276:immunology*; D006678:HIV / Q000235:genetics / Q000276:immunology; D015483:HIV Antibodies / Q000096:biosynthesis / Q000276:immunology; D016655:HIV Core Protein p24 / Q000235:genetics / Q000276:immunology*; D015658:HIV Infections / Q000276:immunology / Q000517:prevention & control / Q000821:virology; D006801:Humans; D007117:Immunization, Secondary; D007273:Injections, Intramuscular; D007371:Interferon-gamma / Q000096:biosynthesis / Q000276:immunology; D008253:Macaca mulatta; D008297:Male; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D015302:Simian Immunodeficiency Virus / Q000187:drug effects* / Q000502:physiology; D014611:Vaccination; D019444:Vaccines, DNA; D019562:Viral Load / Q000187:drug effects
chemical_list: D016915:AIDS Vaccines; D004279:DNA, Viral; D015483:HIV Antibodies; D016655:HIV Core Protein p24; D019444:Vaccines, DNA; D007371:Interferon-gamma