title: Pancreatic lipase inhibitory activity of phlorotannins isolated from Eisenia bicyclis.
abstract: Pancreatic lipase is a potential therapeutic target for the treatment of diet-induced obesity in humans. In an ongoing search for new pancreatic lipase inhibitors from natural sources, a methanolic extract of marine brown algae, Eisenia bicyclis, showed a significant inhibitory effect against pancreatic lipase. Bioassay-guided isolation of this methanolic extract using a pancreatic lipase inhibitory assay led to the isolation and identification of six known phlorotannins: eckol (1), fucofuroeckol A (2), 7-phloroeckol (3), dioxindehydroeckol (4), phlorofucofuroeckol A (5), and dieckol (6). The structures were established on the basis of nuclear magnetic resonance and mass spectrometry spectroscopic data interpretation. Among the isolated phloroglucinol polymers, compounds 2 and 3 showed potent inhibitory effects on pancreatic lipase with IC₅₀ values ranging from 37.2 ± 2.3 to 12.7 ± 1.0 μM, respectively.
authors.affiliation: Department of Food Science and Technology, Pukyong National University, Busan 608-737, Korea.
authors.forename: Sung-Hwan
authors.initials: SH
authors.lastname: Eom
authors.forename: Myung-Suk
authors.initials: MS
authors.lastname: Lee
authors.forename: Eun-Woo
authors.initials: EW
authors.lastname: Lee
authors.forename: Young-Mog
authors.initials: YM
authors.lastname: Kim
authors.forename: Tae Hoon
authors.initials: TH
authors.lastname: Kim
mesh_terms: D000818:Animals; D001572:Benzofurans / Q000302:isolation & purification / Q000494:pharmacology; D004147:Dioxins / Q000302:isolation & purification / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D008049:Lipase / Q000037:antagonists & inhibitors*; D000078183:Oxindoles; D000459:Phaeophyceae / Q000737:chemistry*; D013552:Swine; D013634:Tannins / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: C558314:7-phloroeckol; D001572:Benzofurans; D004147:Dioxins; D000078183:Oxindoles; D013634:Tannins; C503840:dieckol; C580564:dioxindehydroeckol; C060311:eckol; D008049:Lipase