title: The association of a distinct plasma proteomic profile with the cervical high-grade squamous intraepithelial lesion of Uyghur women: a 2D liquid-phase chromatography/mass spectrometry study.
abstract: OBJECTIVE
  To identify plasma protein biomarkers of cervical high-grade squamous intraepithelial lesion (HSIL) of Uyghur women by proteomics approach.
  
  
  METHODS
  Plasma protein samples of Uyghur women with HSIL and chronic cervicitis were analyzed with 2D HPLC followed by detection of target proteins with Linear Trap Quadrupole Mass Spectrometer (LTQ MS/MS).
  
  
  RESULTS
  We detected three upregulated and one downregulated protein peaks representing protein constituents distinguishing HSIL from controls by 2D HPLC, identified 31 target proteins by LTQ MS/MS. Further confirmed analysis with online software IPA® 8.7 and ELISA assay showed APOA1 and mTOR as potential biomarkers.
  
  
  CONCLUSIONS
  A distinct plasma proteomic profile may be associated with HSIL of Uyghur women.
authors.affiliation: Xinjiang Key Laboratory of Molecular Biology and Endemic Diseases, Xinjiang Medical University, Urumqi, PR China.
authors.forename: Xia
authors.initials: X
authors.lastname: Guo
authors.forename: Guzalnur
authors.initials: G
authors.lastname: Abliz
authors.forename: Halidan
authors.initials: H
authors.lastname: Reyimu
authors.forename: Feng
authors.initials: F
authors.lastname: Zhao
authors.forename: Nafeisha
authors.initials: N
authors.lastname: Kadeer
authors.forename: Rukeya
authors.initials: R
authors.lastname: Matsidik
authors.forename: Guizhen
authors.initials: G
authors.lastname: Wu
authors.forename: Abulizi
authors.initials: A
authors.lastname: Abudula
mesh_terms: D000328:Adult; D016632:Apolipoprotein A-I / Q000097:blood* / Q000302:isolation & purification; D044466:Asian People; D014408:Biomarkers, Tumor / Q000097:blood* / Q000302:isolation & purification; D001798:Blood Proteins / Q000302:isolation & purification / Q000378:metabolism; D020968:Brachial Plexus Neuritis; D056148:Chromatography, Reverse-Phase; D055088:Early Detection of Cancer; D005260:Female; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000097:blood / Q000302:isolation & purification; D008875:Middle Aged; D010928:Placental Lactogen; D040901:Proteomics; D058570:TOR Serine-Threonine Kinases / Q000097:blood* / Q000302:isolation & purification; D053719:Tandem Mass Spectrometry; D002583:Uterine Cervical Neoplasms / Q000097:blood* / Q000175:diagnosis; D002575:Uterine Cervicitis / Q000097:blood; D002578:Uterine Cervical Dysplasia / Q000097:blood* / Q000175:diagnosis
chemical_list: C505693:APOA1 protein, human; D016632:Apolipoprotein A-I; D014408:Biomarkers, Tumor; D001798:Blood Proteins; D036341:Intercellular Signaling Peptides and Proteins; D010928:Placental Lactogen; C546842:MTOR protein, human; D058570:TOR Serine-Threonine Kinases