title: T2D risk haplotypes of the TCF7L2 gene in the Czech population sample: the association with free fatty acids composition.
abstract: The association of transcription factor 7-like 2 (TCF7L2) gene variants with the pathogenesis of T2D, gestational diabetes and polycystic ovary syndrome (PCOS) was examined. The study involved 1460 individuals: 347 T2D patients (D); 261 gestational diabetics (G); 147 offspring of T2D (O); 329 women with PCOS, and 376 controls (C). The SNPs: rs7901695; rs7903146; rs12255372 in the TCF7L2 gene were genotyped. Anthropometric and biochemical parameters, oGTT derived indices were assessed. In addition, free fatty acids (FFAs) were evaluated in 183 non-diabetic women. The CTT haplotype showed the strongest association with T2D with OR 1.57, p=0.0003. The frequency of the CTT/CTT haplotype was decreasing in following order: D 10.6, O 9.5, G 6.1, C 5.3 and PCOS 4.9 [%]. Among CTT carriers, significantly decreased levels of oGTT-stimulated insulin and C-peptide as well as proportions of fasting PUFAs were observed. The carriership of CTG/TCG was associated with gestational diabetes, OR 2.59, p=0.036. The association of TCF7L2 haplotypes with T2D and gestational diabetes but not with PCOS was confirmed. Novel association of TCF7L2 with FFAs composition was found.
authors.affiliation: Department of Molecular Endocrinology, Institute of Endocrinology, Prague, Czech Republic. jvcelak@endo.cz
authors.forename: J
authors.initials: J
authors.lastname: Včelák
authors.forename: D
authors.initials: D
authors.lastname: Vejražková
authors.forename: M
authors.initials: M
authors.lastname: Vaňková
authors.forename: P
authors.initials: P
authors.lastname: Lukášová
authors.forename: O
authors.initials: O
authors.lastname: Bradnová
authors.forename: T
authors.initials: T
authors.lastname: Hálková
authors.forename: J
authors.initials: J
authors.lastname: Bešťák
authors.forename: K
authors.initials: K
authors.lastname: Andělová
authors.forename: H
authors.initials: H
authors.lastname: Kvasničková
authors.forename: P
authors.initials: P
authors.lastname: Hoskovcová
authors.forename: K
authors.initials: K
authors.lastname: Vondra
authors.forename: J
authors.initials: J
authors.lastname: Vrbíková
authors.forename: B
authors.initials: B
authors.lastname: Bendlová
mesh_terms: D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D015415:Biomarkers / Q000097:blood; D016009:Chi-Square Distribution; D018153:Czech Republic / Q000453:epidemiology; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000453:epidemiology / Q000235:genetics*; D016640:Diabetes, Gestational / Q000097:blood / Q000453:epidemiology / Q000235:genetics; D005230:Fatty Acids, Nonesterified / Q000097:blood*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D010641:Phenotype; D011085:Polycystic Ovary Syndrome / Q000097:blood / Q000453:epidemiology / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D018570:Risk Assessment; D012307:Risk Factors; D058118:Transcription Factor 7-Like 2 Protein / Q000235:genetics*
chemical_list: D015415:Biomarkers; D005230:Fatty Acids, Nonesterified; C544894:TCF7L2 protein, human; D058118:Transcription Factor 7-Like 2 Protein