title: Expression of melanoma differentiation associated gene 5 is increased in human gastric mucosa infected with Helicobacter pylori.
abstract: Helicobacter pylori infection is associated with gastroduodenal diseases. Melanoma differentiation associated gene 5 (MDA5) plays a role in antiviral host defense. We investigated the effect of H pylori infection on MDA5 expression in human gastric mucosa. Biopsy samples from the antrum and corpus were obtained from 33 patients. MDA5 mRNA and protein were examined by real-time PCR and immunohistochemical staining. Histological gastritis was graded according to updated Sydney System. MDA5 mRNA was significantly increased in the antrum infected with H pylori. MDA5 protein positively stained in infiltrating mononuclear cells. MDA5 mRNA expression was significantly correlated with the grade of glandular atrophy (rs = 0.767) and intestinal metaplasia (rs = 0.748) in the corpus with H pylori infection. These results indicate that MDA5 may be involved in innate immune reactions against H pylori and associate with glandular atrophy and intestinal metaplasia in patients with H pylori infection.
authors.affiliation: Department of Gastroenterology, Hirosaki University Graduate School of Medicine, Hirosaki, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Tatsuta
authors.forename: Tadaatsu
authors.initials: T
authors.lastname: Imaizumi
authors.forename: Tadashi
authors.initials: T
authors.lastname: Shimoyama
authors.forename: Manabu
authors.initials: M
authors.lastname: Sawaya
authors.forename: Tanji
authors.initials: T
authors.lastname: Kunikazu
authors.forename: Tomoh
authors.initials: T
authors.lastname: Matsumiya
authors.forename: Hidemi
authors.initials: H
authors.lastname: Yoshida
authors.forename: Kei
authors.initials: K
authors.lastname: Satoh
authors.forename: Shinsaku
authors.initials: S
authors.lastname: Fukuda
mesh_terms: D000368:Aged; D001284:Atrophy; D001706:Biopsy; D016022:Case-Control Studies; D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases / Q000032:analysis* / Q000235:genetics; D005260:Female; D005753:Gastric Mucosa / Q000737:chemistry* / Q000276:immunology / Q000382:microbiology* / Q000473:pathology; D005756:Gastritis / Q000276:immunology / Q000382:microbiology* / Q000473:pathology; D016481:Helicobacter Infections / Q000276:immunology / Q000382:microbiology*; D016480:Helicobacter pylori / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007113:Immunity, Innate; D007150:Immunohistochemistry; D000072640:Interferon-Induced Helicase, IFIH1; D007564:Japan; D008297:Male; D008679:Metaplasia; D008875:Middle Aged; D011446:Prospective Studies; D011706:Pyloric Antrum / Q000737:chemistry* / Q000276:immunology / Q000382:microbiology* / Q000473:pathology; D012333:RNA, Messenger / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction; D011971:Receptors, Immunologic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012720:Severity of Illness Index; D051196:Toll-Like Receptor 3 / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D011971:Receptors, Immunologic; C495344:TLR3 protein, human; D051196:Toll-Like Receptor 3; C452820:RIGI protein, human; C452815:IFIH1 protein, human; D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases; D000072640:Interferon-Induced Helicase, IFIH1