title: Purification and antioxidant activity of a polysaccharide from bulbs of Fritillaria ussuriensis Maxim.
abstract: A water-soluble polysaccharide (FUP-1) was obtained from Fritillaria ussuriensis Maxim through warm water extraction, ethanol precipitation, anion-exchange and gel-permeation chromatography. Structural analysis of this compound revealed that the average molecular weight of FUP-1 was 4.1×10(4)Da, and the major monosaccharide component was xylose, followed by glucose and galactose, at low levels. In vitro antioxidant assays showed that FUP-1 exhibited strong hydroxyl, superoxide anion and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activities. During in vivo antioxidant testing, the administration of FUP-1 could largely attenuate the increase in d-gal-induced malondialdehyde (MDA) and renew the activities of superoxide dismutase (SOD) in serums as well as glutathione peroxidase (GSH-Px) and the total antioxidant capacity (T-AOC) in the livers of d-gal-treated mice. Overall, the results of the present study indicate that FUP-1 exerts antioxidant activity not only through its own radical-scavenging activity but also by boosting the enzymatic and nonenzymatic antioxidant defense system of the host.
authors.affiliation: Special Education Collage, Changchun University, Changchun 130022, PR China.
authors.forename: Chunhong
authors.initials: C
authors.lastname: Liu
authors.forename: Jinkang
authors.initials: J
authors.lastname: Chang
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Shengyu
authors.initials: S
authors.lastname: Li
mesh_terms: D000818:Animals; D005591:Chemical Fractionation / Q000379:methods*; D016166:Free Radical Scavengers / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D005609:Free Radicals / Q000378:metabolism; D031410:Fritillaria / Q000737:chemistry*; D005690:Galactose / Q000494:pharmacology; D005979:Glutathione Peroxidase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects; D018517:Plant Roots / Q000737:chemistry*; D011134:Polysaccharides / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D016166:Free Radical Scavengers; D005609:Free Radicals; D011134:Polysaccharides; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005690:Galactose