title: MicroRNA-320a suppresses human colon cancer cell proliferation by directly targeting β-catenin.
abstract: Recent profile studies of microRNA (miRNA) expression have documented a deregulation of miRNA (miR-320a) in human colorectal carcinoma. However, its expression pattern and underlying mechanisms in the development and progression of colorectal carcinoma has not been elucidated clearly. Here, we performed real-time PCR to examine the expression levels of miR-320a in colon cancer cell lines and tumor tissues. And then, we investigated its biological functions in colon cancer cells by a gain of functional strategy. Further more, by the combinational approaches of bioinformatics and experimental validation, we confirmed target associations of miR-320a in colorectal carcinoma. Our results showed that miR-320a was frequently downregulated in cancer cell lines and colon cancer tissues. And we demonstrated that miR-320a restoration inhibited colon cancer cell proliferation and β-catenin, a functionally oncogenic molecule was a direct target gene of miR-320a. Finally, the data of real-time PCR showed the reciprocal relationship between miR-320a and β-catenin's downstream genes in colon cancer tissues. These findings indicate that miR-320a suppresses the growth of colon cancer cells by directly targeting β-catenin, suggesting its application in prognosis prediction and cancer treatment.
authors.affiliation: State Key Laboratory of Cancer Biology, Department of Biochemistry and Molecular Biology, Fourth Military Medical University, 710032 Xi'an, China.
authors.forename: Jian-Yong
authors.initials: JY
authors.lastname: Sun
authors.forename: Yi
authors.initials: Y
authors.lastname: Huang
authors.forename: Ji-Peng
authors.initials: JP
authors.lastname: Li
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhang
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Yan-Ling
authors.initials: YL
authors.lastname: Meng
authors.forename: Bo
authors.initials: B
authors.lastname: Yan
authors.forename: Yong-Qian
authors.initials: YQ
authors.lastname: Bian
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.forename: Wei-Zhong
authors.initials: WZ
authors.lastname: Wang
authors.forename: An-Gang
authors.initials: AG
authors.lastname: Yang
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003110:Colonic Neoplasms / Q000235:genetics* / Q000473:pathology*; D015972:Gene Expression Regulation, Neoplastic; D018390:Gene Targeting; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D051176:beta Catenin / Q000235:genetics*
chemical_list: C572916:MIRN320 microRNA, human; D035683:MicroRNAs; D051176:beta Catenin