title: Single-molecule dynamics of transcription of the lar promoter.
abstract: We measured the in vivo production of RNA molecules tagged with MS2d-GFP in Escherichia coli, driven by the lar promoter, under weak and medium induction. The distributions of intervals between consecutive productions of RNAs are found to be sub-exponential, and the process of RNA production is found to be sub-Poissonian. We discuss possible models of transcription initiation and, based on our results and previous in vitro measurements, find that a sequential two-step model of transcription initiation at the promoter region explains the results well.
authors.affiliation: Computational Systems Biology Research Group, Department of Signal Processing, Tampere University of Technology, Tampere, Finland.
authors.forename: M
authors.initials: M
authors.lastname: Kandhavelu
authors.forename: A
authors.initials: A
authors.lastname: Häkkinen
authors.forename: O
authors.initials: O
authors.lastname: Yli-Harja
authors.forename: A S
authors.initials: AS
authors.lastname: Ribeiro
mesh_terms: D004926:Escherichia coli / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007700:Kinetics; D007763:Lac Operon; D017909:Levivirus / Q000378:metabolism; D018613:Microscopy, Confocal; D016012:Poisson Distribution; D011401:Promoter Regions, Genetic; D012329:RNA, Bacterial / Q000235:genetics; D013997:Time Factors; D014158:Transcription, Genetic; D015533:Transcriptional Activation
chemical_list: D012329:RNA, Bacterial; D049452:Green Fluorescent Proteins