title: Large-scale identification of selenium metabolites by online size-exclusion-reversed phase liquid chromatography with combined inductively coupled plasma (ICP-MS) and electrospray ionization linear trap-Orbitrap mass spectrometry (ESI-MS(n)).
abstract: A method was developed for the comprehensive cartography of the selenium metabolites synthesized in the process of conversion of selenite [Se(iv)] into organic compounds and enrichment of yeast with selenium. The number of compounds detected was considerably increased (49) owing to the optimization of the fractionation procedure and the use of UPLC. The increased purity of the minor selenoorganic amino acids and oligopeptides allowed successful on-line de-novo identification based on the exact mass (<1 ppm) and fragmentation (MS(2)/MS(3)) mass spectra obtained with high resolution and high mass accuracy. The quality of mass spectra allowed the re-interpretation of previously reported structures of some selenium species.
authors.affiliation: CNRS/UPPA, Laboratoire de Chimie Analytique Bioinorganique et Environnement, UMR5254, Hélioparc, 2 av Pr Angot, 64053 Pau, France. hugues.preudhomme@univ-pau.fr
authors.forename: Hugues
authors.initials: H
authors.lastname: Preud'homme
authors.forename: Johann
authors.initials: J
authors.lastname: Far
authors.forename: Sandra
authors.initials: S
authors.lastname: Gil-Casal
authors.forename: Ryszard
authors.initials: R
authors.lastname: Lobinski
mesh_terms: D002850:Chromatography, Gel / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods; D053858:Metabolic Networks and Pathways; D009942:Organometallic Compounds / Q000737:chemistry* / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D012643:Selenium / Q000737:chemistry* / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D009942:Organometallic Compounds; D012643:Selenium