title: Endosulfan exposure inhibits brain AChE activity and impairs swimming performance in adult zebrafish (Danio rerio).
abstract: Endosulfan is a broad spectrum organochlorine pesticide that is still widely in use in many developing countries. Following application, endosulfan can get to watercourses through surface runoff from agricultural fields and disturb the non-target aquatic animals including freshwater fish species. Given that the activity of the enzyme acetylcholinesterase (AChE) is one of the most recurrently used biomarkers of exposure to pesticides and there are controversial results concerning the effects of endosulfan exposure and AChE activity in fish, the aim of the present study was to evaluate the effects of endosulfan in brain AChE activity and its gene expression pattern using adult zebrafish (Danio rerio) as an animal model. Moreover, we have analyzed the effects of endosulfan exposure in different parameters of zebrafish swimming activity and in long-term memory formation. After 96 h of exposition, fish in the 2.4 μg endosulfan/L group presented a significant decrease in AChE activity (9.44 ± 1.038 μmol SCh h(-1) mg protein(-1); p=0.0205) when compared to the control group (15.87 ± 1.768 μmol SCh h(-1) mg protein(-1); p=0.0205) which corresponds to approximately 40%. The down-regulation of brain AChE activity is not directly related with the transcriptional control as demonstrated by the RT-qPCR analysis. Our results reinforce AChE activity inhibition as a pathway of endosulfan-induced toxicity in brain of fish species. In addition, exposure to 2.4 μg endosulfan/L during 96 h impaired all exploratory parameters evaluated: decreased line crossings (≈21%, 273.7 ± 28.12 number of line crossings compared to the control group 344.6 ± 21.30, p=0.0483), traveled distance (≈20%, 23.44 ± 2.127 m compared to the control group 29.39 ± 1.585, p=0.0281), mean speed (≈25%, 0.03 ± 0.003 m/s compared to the control group 0.04 ± 0.002, p=0.0275) and body turn angle (≈21%, 69,940 ± 4871 absolute turn angle compared to the control group 88,010 ± 4560, p=0.0114). These results suggest that endosulfan exposure significantly impairs animals' exploratory performance, and potentially compromises their ecological and interspecific interaction. Our results also showed that the same endosulfan exposure did not compromise animals' performance in the inhibitory avoidance apparatus. These findings provide further evidence of the deleterious effects of endosulfan exposure in the nervous system.
authors.affiliation: Laboratório de Biologia Genômica e Molecular, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul, Avenida Ipiranga, 6681, 90619-900 Porto Alegre, RS, Brazil.
authors.forename: Vanessa Maynart
authors.initials: VM
authors.lastname: Pereira
authors.forename: Josiane Woutheres
authors.initials: JW
authors.lastname: Bortolotto
authors.forename: Luiza Wilges
authors.initials: LW
authors.lastname: Kist
authors.forename: Mariana Barbieri de
authors.initials: MB
authors.lastname: Azevedo
authors.forename: Rachel Seemann
authors.initials: RS
authors.lastname: Fritsch
authors.forename: Renata da Luz
authors.initials: Rda L
authors.lastname: Oliveira
authors.forename: Talita Carneiro Brandão
authors.initials: TC
authors.lastname: Pereira
authors.forename: Carla Denise
authors.initials: CD
authors.lastname: Bonan
authors.forename: Monica Ryff
authors.initials: MR
authors.lastname: Vianna
authors.forename: Maurício Reis
authors.initials: MR
authors.lastname: Bogo
mesh_terms: D000110:Acetylcholinesterase / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D001921:Brain / Q000187:drug effects* / Q000201:enzymology / Q000503:physiopathology; D002800:Cholinesterase Inhibitors / Q000633:toxicity*; D004726:Endosulfan / Q000633:toxicity*; D005106:Exploratory Behavior / Q000187:drug effects; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D007306:Insecticides / Q000633:toxicity*; D008297:Male; D008568:Memory / Q000187:drug effects; D009043:Motor Activity / Q000187:drug effects*; D012333:RNA, Messenger / Q000378:metabolism; D013550:Swimming; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D015027:Zebrafish / Q000378:metabolism*
chemical_list: D002800:Cholinesterase Inhibitors; D007306:Insecticides; D012333:RNA, Messenger; D014874:Water Pollutants, Chemical; D000110:Acetylcholinesterase; D004726:Endosulfan