title: Multiplexed detection of cardiac biomarkers in serum with nanowire arrays using readout ASIC.
abstract: Early detection of cardiac biomarkers for diagnosis of heart attack is the key to saving lives. Conventional method of detection like the enzyme-linked immunosorbent assay (ELISA) is time consuming and low in sensitivity. Here, we present a label-free detection system consisting of an array of silicon nanowire sensors and an interface readout application specific integrated circuit (ASIC). This system provides a rapid solution that is highly sensitive and is able to perform direct simultaneous-multiplexed detection of cardiac biomarkers in serum. Nanowire sensor arrays were demonstrated to have the required selectivity and sensitivity to perform multiplexed detection of 100 fg/ml troponin T, creatine kinase MM, and creatine kinase MB in serum. A good correlation between measurements from a probe station and the readout ASIC was obtained. Our detection system is expected to address the existing limitations in cardiac health management that are currently imposed by the conventional testing platform, and opens up possibilities in the development of a miniaturized device for point-of-care diagnostic applications.
authors.affiliation: Institute of Microelectronics, A*STAR (Agency for Science, Technology and Research), 11 Science Park Road, Singapore 117685, Singapore; School of Laboratory Medicine, Hubei University of Chinese Medicine, 1 Huangjia Lake West Road, Wuhan 430065, China. Electronic address: gjzhangtj@hotmail.com.
authors.forename: Guo-Jun
authors.initials: GJ
authors.lastname: Zhang
authors.affiliation: Institute of Microelectronics, A*STAR (Agency for Science, Technology and Research), 11 Science Park Road, Singapore 117685, Singapore. Electronic address: chaitc@ime.a-star.edu.sg.
authors.forename: Kevin Tshun Chuan
authors.initials: KTC
authors.lastname: Chai
authors.affiliation: Institute of Microelectronics, A*STAR (Agency for Science, Technology and Research), 11 Science Park Road, Singapore 117685, Singapore.
authors.forename: Henry Zhan Hong
authors.initials: HZH
authors.lastname: Luo
authors.affiliation: Institute of Microelectronics, A*STAR (Agency for Science, Technology and Research), 11 Science Park Road, Singapore 117685, Singapore.
authors.forename: Joon Min
authors.initials: JM
authors.lastname: Huang
authors.affiliation: Institute of Microelectronics, A*STAR (Agency for Science, Technology and Research), 11 Science Park Road, Singapore 117685, Singapore.
authors.forename: Ignatius Guang Kai
authors.initials: IGK
authors.lastname: Tay
authors.affiliation: Institute of Microelectronics, A*STAR (Agency for Science, Technology and Research), 11 Science Park Road, Singapore 117685, Singapore.
authors.forename: Andy Eu-Jin
authors.initials: AE
authors.lastname: Lim
authors.affiliation: Institute of Microelectronics, A*STAR (Agency for Science, Technology and Research), 11 Science Park Road, Singapore 117685, Singapore.
authors.forename: Minkyu
authors.initials: M
authors.lastname: Je
mesh_terms: D055910:Antibodies, Immobilized; D015415:Biomarkers / Q000097:blood*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods / Q000706:statistics & numerical data; D052279:Creatine Kinase, MB Form / Q000097:blood; D052283:Creatine Kinase, MM Form / Q000097:blood; D004867:Equipment Design; D006331:Heart Diseases / Q000097:blood / Q000175:diagnosis; D006801:Humans; D057230:Limit of Detection; D009203:Myocardial Infarction / Q000097:blood* / Q000175:diagnosis*; D053770:Nanowires; D019095:Point-of-Care Systems; D012825:Silicon; D020107:Troponin T / Q000097:blood
chemical_list: D055910:Antibodies, Immobilized; D015415:Biomarkers; D020107:Troponin T; D052279:Creatine Kinase, MB Form; D052283:Creatine Kinase, MM Form; D012825:Silicon