title: Proliferation and skeletal myotube formation capability of C2C12 and H9c2 cells on isotropic and anisotropic electrospun nanofibrous PHB scaffolds.
abstract: This study aims at investigating the behavior in terms of the proliferation and skeletal muscle differentiation capability of two myoblastic cell lines, C2C12 and H9c2, on both isotropic and anisotropic electrospun nanofibrous poly(hydroxybutyrate) (PHB) scaffolds, as well as on PHB films and polystyrene controls. After a careful characterization of the matrices in terms of surface morphology, surface roughness and mechanical properties, the proliferation rate and the capability of the two cell lines to form skeletal myotubes were evaluated. Genetic analyses were also performed in order to assess the differentiation level of the cells on the different substrates. We demonstrated that the aligned nanofibrous mesh decreases the proliferation activity and provides a higher differentiative stimulus. We also clarified how the nanofibrous substrate influences myotube formation, and quantified a series of myotube-related parameters for both C2C12 and H9c2 cells.
authors.affiliation: Biorobotics Institute, Polo Sant'Anna Valdera, Scuola Superiore Sant'Anna, Pontedera (Pisa), Italy.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Ricotti
authors.forename: Alessandro
authors.initials: A
authors.lastname: Polini
authors.forename: Giada G
authors.initials: GG
authors.lastname: Genchi
authors.forename: Gianni
authors.initials: G
authors.lastname: Ciofani
authors.forename: Donata
authors.initials: D
authors.lastname: Iandolo
authors.forename: Helena
authors.initials: H
authors.lastname: Vazão
authors.forename: Virgilio
authors.initials: V
authors.lastname: Mattoli
authors.forename: Lino
authors.initials: L
authors.lastname: Ferreira
authors.forename: Arianna
authors.initials: A
authors.lastname: Menciassi
authors.forename: Dario
authors.initials: D
authors.lastname: Pisignano
mesh_terms: D016880:Anisotropy; D001672:Biocompatible Materials / Q000138:chemical synthesis*; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D004563:Electrochemistry / Q000379:methods; D006801:Humans; D006885:Hydroxybutyrates / Q000737:chemistry*; D008422:Materials Testing; D018485:Muscle Fibers, Skeletal / Q000166:cytology* / Q000502:physiology; D032448:Myoblasts, Skeletal / Q000166:cytology* / Q000502:physiology; D011091:Polyesters / Q000737:chemistry*; D000091402:Prohibitins; D054457:Tissue Scaffolds; D000094122:Polyhydroxybutyrates
chemical_list: D001672:Biocompatible Materials; D006885:Hydroxybutyrates; D011091:Polyesters; D000091402:Prohibitins; C000717328:PHB protein, human; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates