title: Panthenyl triacetate transformation, stimulation of metabolic pathways, and wound-healing properties in the human skin.
abstract: Vitamin B5 and its derivatives are well known in personal care applications and are often used in wound healing and soothing compositions. However, little is known about the biochemical pathways involved. A better knowledge of these pathways would help to understand some of the mechanisms of action and suggest further applications. We have investigated the transformation of D-panthenyl triacetate (PTA) into D-panthenol (PAN) and its skin diffusion on human volunteers by Raman spectroscopy. Additionally, we have utilized human skin biopsies and quantitative RT-PCR to demonstrate the effect of PTA compared to PAN on 27 metabolic markers when introduced at 2% in a cosmetic emulsion. Then we conducted a double-blind clinical study to measure the effect of PTA compared to PAN on wound healing, measured by transepidermal water loss (TEWL), when incorporated at 3% in a cosmetic emulsion. Results show de-acetylation of PTA into PAN and an increased activity of PTA compared to PNA over time in the skin. Metabolic marker analysis demonstrates stimulation of energetic pathways such as glycolysis and the citric acid cycle, but also of synthesis pathways such as isoprenoids and lipid synthesis, by PTA and PAN. Finally, the clinical study demonstrates a statistically significant effect by PTA on wound healing after 72 hours when compared to a saline treatment. Statistical significance was not achieved by PAN or a placebo treatment. Due to the differences between PTA and PAN action, different applications in personal care products can be suggested. Moreover, PTA seems more effective than PAN for a long-lasting wound healing action.
authors.affiliation: Induchem AG, Industriestrasse 8a, CH-8604, Volketswil, Switzerland. giorgio.dellacqua@induchem.com
authors.forename: Giorgio
authors.initials: G
authors.lastname: Dell'Acqua
authors.forename: Kuno
authors.initials: K
authors.lastname: Schweikert
mesh_terms: D000085:Acetates / Q000493:pharmacokinetics / Q000494:pharmacology*; D000328:Adult; D001711:Biotransformation; D004311:Double-Blind Method; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010205:Pantothenic Acid / Q000031:analogs & derivatives* / Q000493:pharmacokinetics / Q000494:pharmacology*; D060888:Real-Time Polymerase Chain Reaction; D012867:Skin / Q000187:drug effects* / Q000378:metabolism; D013059:Spectrum Analysis, Raman; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000085:Acetates; D010205:Pantothenic Acid