title: Effect of GCSB-5, a Herbal Formulation, on Monosodium Iodoacetate-Induced Osteoarthritis in Rats.
abstract: Therapeutic effects of GCSB-5 on osteoarthritis were measured by the amount of glycosaminoglycan in rabbit articular cartilage explants in vitro, in experimental osteoarthritis induced by intra-articular injection of monoiodoacetate in rats in vivo. GCSB-5 was orally administered for 28 days. In vitro, GCSB-5 inhibited proteoglycan degradation. GCSB-5 significantly suppressed the histological changes in monoiodoacetate-induced osteoarthritis. Matrix metalloproteinase (MMP) activity, as well as, the levels of serum tumor necrosis factor-α, cyclooxygenase-2, inducible nitric oxide synthase protein, and mRNA expressions were attenuated by GCSB-5, whereas the level of interleukin-10 was potentiated. By GCSB-5, the level of nuclear factor-κB p65 protein expression was significantly attenuated but, on the other hand, the level of inhibitor of κB-α protein expression was increased. These results indicate that GCSB-5 is a potential therapeutic agent for the protection of articular cartilage against progression of osteoarthritis through inhibition of MMPs activity, inflammatory mediators, and NF-κB activation.
authors.affiliation: School of Pharmacy, Sungkyunkwan University, Suwon, Gyeonggi-do 440-746, Republic of Korea.
authors.forename: Joon-Ki
authors.initials: JK
authors.lastname: Kim
authors.forename: Sang-Won
authors.initials: SW
authors.lastname: Park
authors.forename: Jung-Woo
authors.initials: JW
authors.lastname: Kang
authors.forename: Yu-Jin
authors.initials: YJ
authors.lastname: Kim
authors.forename: Sung Youl
authors.initials: SY
authors.lastname: Lee
authors.forename: Joonshik
authors.initials: J
authors.lastname: Shin
authors.forename: Sangho
authors.initials: S
authors.lastname: Lee
authors.forename: Sun-Mee
authors.initials: SM
authors.lastname: Lee