title: BIOTECON diagnostics foodproof Listeria monocytogenes Detection Kit, 5' nuclease in combination with the foodproof ShortPrep II Kit.
abstract: A method was developed for the detection of Listeria monocytogenes in food. The method is based on real-time PCR using hydrolysis probes (5' Nuclease). This advanced PCR method was designed to reduce the time necessary to achieve results from PCR reactions and to enable the user to monitor the amplification of the PCR product simultaneously, in real-time. After DNA isolation using the BIOTECON foodproof ShortPrep II Kit designed for the rapid preparation of L. monocytogenes DNA for direct use in PCR, the real-time detection of L. monocytogenes DNA is carried out using the foodproof Listeria monocytogenes Detection Kit. The kit provides primers and hydrolysis probes for sequence-specific detection, convenient premixed reagents, and controls for reliable interpretation of results. For the internal comparison study, three different foods (soft cheese, coalfish, and smoked ham) were analyzed, chosen from the 15 food groups recommended by the AOAC Research Institute for detection of L. monocytogenes. From each food, 20 samples were inoculated with a low level (1-10 CFU/25 g) and 20 samples with a high level (10-50 CFU/25 g) of L. monocytogenes. Additionally, five nonspiked samples were prepared from each food. Depending on the matrix, the food samples were examined with the test kits and compared with the cultural methods according to the U.S. Food and Drug Administration's Bacteriological Analytical Manual or the U.S. Department of Agriculture/Food Safety and Inspection Service Microbiology Laboratory Guidebook.
authors.affiliation: BIOTECON Diagnostics GmbH, Hermannswerder Haus 17, 14473 Potsdam, Germany.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Junge
authors.forename: Cordt
authors.initials: C
authors.lastname: Grönewald
authors.forename: Kornelia
authors.initials: K
authors.lastname: Berghof-Jäger
mesh_terms: D000818:Animals; D002611:Cheese / Q000032:analysis; D015169:Colony Count, Microbial; D003470:Culture Media; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D003851:Deoxyribonucleases / Q000737:chemistry*; D005399:Fishes; D005516:Food Microbiology / Q000295:instrumentation* / Q000379:methods; D006868:Hydrolysis; D007202:Indicators and Reagents; D008089:Listeria monocytogenes / Q000737:chemistry*; D008460:Meat / Q000032:analysis; D011933:Reagent Kits, Diagnostic; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012015:Reference Standards; D015203:Reproducibility of Results; D013552:Swine
chemical_list: D003470:Culture Media; D017931:DNA Primers; D004269:DNA, Bacterial; D007202:Indicators and Reagents; D011933:Reagent Kits, Diagnostic; D003851:Deoxyribonucleases