title: Osteopontin and symmetric dimethylarginine plasma levels in solitary functioning kidney in children.
abstract: OBJECTIVE
  The present study aimed to examine whether plasma osteopontin (pOPN) and symmetric dimethylarginine (pSDMA) are useful biomarkers of renal dysfunction in children with solitary functioning kidney (SFK).
  
  
  METHODS
  We measured circulating pOPN and pSDMA in 51 patients with SFK and no other urinary defects. Patients were subdivided into two groups: primary SFK (pSFK) - unilateral renal agenesis (URA), and secondary SFK (sSFK) - unilateral nephrectomy. The control group (C) contained 21 healthy children, with mean age 9.92 ± 4.85 years. Immunoenzymatic ELISA commercial kits were used to measure pOPN and pSDMA concentrations.
  
  
  RESULTS
  Plasma osteopontin and pSDMA levels in children with SFK were higher than those in healthy participants (p < 0.05). There was no difference in pOPN and pSDMA concentrations between patients with pSFK and those with sSFK (p > 0.05). Receiver operator characteristic analyses performed to define the diagnostic efficiency of serum creatinine, pOPN and pSDMA in identifying children with C(cr)  < 90 mL/min/1.73 m(2) among all examined children revealed no differences between all three AUCs (p > 0.05).
  
  
  CONCLUSIONS
  Increased pOPN and pSDMA levels were observed in children with SFK. Both pOPN and pSDMA correlated with eGFR; however, the sensitivity and specificity of those markers were not better than those of creatinine.
authors.affiliation: Department of Pediatrics and Nephrology, Medical University of Białystok, Białystok, Poland. katarzyna.taranta@wp.pl
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Taranta-Janusz
authors.forename: Anna
authors.initials: A
authors.lastname: Wasilewska
authors.forename: Justyna
authors.initials: J
authors.lastname: Stypułkowska
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Sutuła
mesh_terms: D000293:Adolescent; D001120:Arginine / Q000031:analogs & derivatives* / Q000097:blood; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D000013:Congenital Abnormalities / Q000097:blood; D003404:Creatinine / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007223:Infant; D007668:Kidney / Q000002:abnormalities; D007674:Kidney Diseases / Q000097:blood / Q000150:complications / Q000151:congenital / Q000175:diagnosis* / Q000209:etiology / Q000601:surgery; D007677:Kidney Function Tests / Q000379:methods*; D008297:Male; D009392:Nephrectomy; D053495:Osteopontin / Q000097:blood*; D012372:ROC Curve; D012189:Retrospective Studies
chemical_list: D015415:Biomarkers; D053495:Osteopontin; C024917:symmetric dimethylarginine; D001120:Arginine; D003404:Creatinine