title: Damaging effects of lyticase on Candida albicans and changes in the response of rat alveolar macrophages to the contact with yeast-like fungi.
abstract: Changes in the cell wall of yeast-like fungi Candida albicans caused by bacterial enzyme lyticase were studied under an electron microscope. The results were compared with the findings on phagocytosis of lyticase-treated Candida albicans by rat alveolar macrophages. It was shown that destruction of the mannan and fibrous outer layers of the cell wall of yeast-like fungi treated with the enzyme led to the release of intracellular organelles and their content. Lyticase treatment reduced the resistance of Candida albicans to intracellular digestion by macrophages contributing to completion of phagocytosis.
authors.affiliation: M. P. Chumakov Institute of Poliomyelitis and Viral Encephalites, Russian Academy of Medical Sciences, Moscow region, Russia.
authors.forename: I A
authors.initials: IA
authors.lastname: Morozov
authors.forename: N P
authors.initials: NP
authors.lastname: Sachivkina
authors.forename: E G
authors.initials: EG
authors.lastname: Kravtsov
authors.forename: E A
authors.initials: EA
authors.lastname: Vasilyeva
authors.forename: I V
authors.initials: IV
authors.lastname: Anokhina
authors.forename: N V
authors.initials: NV
authors.lastname: Yashina
authors.forename: M V
authors.initials: MV
authors.lastname: Dalin
mesh_terms: D000818:Animals; D002176:Candida albicans / Q000187:drug effects* / Q000648:ultrastructure; D005658:Fungi / Q000187:drug effects / Q000648:ultrastructure; D004693:Glucan Endo-1,3-beta-D-Glucosidase / Q000378:metabolism*; D016676:Macrophages, Alveolar / Q000382:microbiology*; D046529:Microscopy, Electron, Transmission; D009097:Multienzyme Complexes / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000378:metabolism*; D010587:Phagocytosis; D051381:Rats
chemical_list: D009097:Multienzyme Complexes; C025310:lyticase; D004693:Glucan Endo-1,3-beta-D-Glucosidase; D010447:Peptide Hydrolases