title: Characterization of bovine induced pluripotent stem cells by lentiviral transduction of reprogramming factor fusion proteins.
abstract: Pluripotent stem cells from domesticated animals have potential applications in transgenic breeding. Here, we describe induced pluripotent stem (iPS) cells derived from bovine fetal fibroblasts by lentiviral transduction of Oct4, Sox2, Klf4 and c-Myc defined-factor fusion proteins. Bovine iPS cells showed typical colony morphology, normal karyotypes, stained positively for alkaline phosphatase (AP) and expressed Oct4, Nanog and SSEA1. The CpG in the promoter regions of Oct4 and Nanog were highly unmethylated in bovine iPS cells compared to the fibroblasts. The cells were able to differentiate into cell types of all three germ layers in vitro and in vivo. In addition, these cells were induced into female germ cells under defined culture conditions and expressed early and late female germ cell-specific genes Vasa, Dazl, Gdf9, Nobox, Zp2, and Zp3. Our data suggest that bovine iPS cells were generated from bovine fetal fibroblasts with defined-factor fusion proteins mediated by lentivirus and have potential applications in bovine transgenic breeding and gene-modified animals.
authors.affiliation: Anhui Local Livestock Genetic Resources Conservation and Breeding Laboratory, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China. caohongguo1@yahoo.com.cn
authors.forename: Hongguo
authors.initials: H
authors.lastname: Cao
authors.forename: Pan
authors.initials: P
authors.lastname: Yang
authors.forename: Yong
authors.initials: Y
authors.lastname: Pu
authors.forename: Xueping
authors.initials: X
authors.lastname: Sun
authors.forename: Huiqun
authors.initials: H
authors.lastname: Yin
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yunhai
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yunsheng
authors.initials: Y
authors.lastname: Li
authors.forename: Ya
authors.initials: Y
authors.lastname: Liu
authors.forename: Fugui
authors.initials: F
authors.lastname: Fang
authors.forename: Zijun
authors.initials: Z
authors.lastname: Zhang
authors.forename: Yong
authors.initials: Y
authors.lastname: Tao
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002417:Cattle; D065150:Cellular Reprogramming / Q000235:genetics; D005347:Fibroblasts / Q000166:cytology; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism*; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000378:metabolism; D016086:Lentivirus / Q000235:genetics*; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism; D055748:SOXB1 Transcription Factors / Q000235:genetics / Q000378:metabolism; D014161:Transduction, Genetic / Q000379:methods*
chemical_list: D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors; D050814:Octamer Transcription Factor-3; D016271:Proto-Oncogene Proteins c-myc; D055748:SOXB1 Transcription Factors; D000469:Alkaline Phosphatase