title: In vivo anthelmintic activity of Clerodendrum colebrookianum Walp., a traditionally used taenicidal plant in Northeast India.
abstract: Although there are several effective drugs available for the treatment of intestinal helminths, the fact remains that they continue to remain out of reach to a vast majority of people in the world, especially in developing countries. On the other hand, there are a great many herbal remedies that are effective against common intestinal worms and are easily available to common people in developing countries. Clerodendrum colebrookianum Walp. (Lamiaceae) is a perennial shrub which is native to South and Southeast Asia. Traditionally, the leaves of this plant are used by the indigenous people of Northeast India as a remedy for the treatment of intestinal tapeworm infections. The aim of this study was to evaluate and authenticate the anthelmintic efficacy of C. colebrookianum leaf extract in experimentally induced Hymenolepis diminuta (a zoonotic tapeworm) infections in Wistar rats. The efficacy of the plant extract was assessed by monitoring the eggs per gram of faeces (EPG) counts and worm recovery rates of experimental animals, following treatment with the leaf extract of this plant at three different doses, i.e. 200, 400 and 800 mg/kg body weight, each given singly for 5 days. The results obtained revealed that the leaf extract of C. colebrookianum possesses a dose-dependent efficacy against the larval, immature and adult stages of H. diminuta. However, the efficacy of the extract was found to be considerably high only against the adult stages of the parasite. For this stage, a single 800-mg/kg dose of extract, given for 5 days, resulted into 68.42% reduction in the EPG counts and 62.50% reduction in the worm counts in the extract-treated group of animals, as compared to the control. The reference drug, praziquantel (5 mg/kg, single dose), however, showed slightly better efficacy and caused 95.16 and 87.00% reductions in the EPG and worm counts of treated animals, respectively. Unlike the adult stages, the efficacy of the plant extract was recorded to be comparatively low against the larval and immature stages of the parasite, as the treatment of animals with the highest dose of extract (800 mg/kg) could cause only 37.50 and 54.00% reductions in worm counts, respectively. The experimental evidence obtained in this study suggests that leaves of C. colebrookianum possess significant anthelminthic properties and supports their use against intestinal tapeworm infections in traditional medicine.
authors.affiliation: Department of Zoology, North-Eastern Hill University, Shillong 793 022, India. akynehu@hotmail.com
authors.forename: Arun K
authors.initials: AK
authors.lastname: Yadav
authors.lastname: Temjenmongla
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000008:administration & dosage* / Q000302:isolation & purification / Q000494:pharmacology; D032409:Clerodendrum / Q000737:chemistry*; D004195:Disease Models, Animal; D005243:Feces / Q000469:parasitology; D005260:Female; D006925:Hymenolepiasis / Q000188:drug therapy* / Q000469:parasitology; D048190:Hymenolepis diminuta / Q000187:drug effects / Q000302:isolation & purification; D007194:India; D008297:Male; D010270:Parasite Egg Count; D010936:Plant Extracts / Q000008:administration & dosage* / Q000302:isolation & purification / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D011223:Praziquantel / Q000008:administration & dosage; D051381:Rats; D017208:Rats, Wistar; D016896:Treatment Outcome
chemical_list: D000871:Anthelmintics; D010936:Plant Extracts; D011223:Praziquantel