title: A competitive chemiluminescence enzyme immunoassay for rapid and sensitive determination of enrofloxacin.
abstract: With alkaline phosphatase (ALP)-adamantane (AMPPD) system as the chemiluminescence (CL) detection system, a highly sensitive, specific and simple competitive chemiluminescence enzyme immunoassay (CLEIA) was developed for the measurement of enrofloxacin (ENR). The physicochemical parameters, such as the chemiluminescent assay mediums, the dilution buffer of ENR-McAb, the volume of dilution buffer, the monoclonal antibody concentration, the incubation time, and other relevant variables of the immunoassay have been optimized. Under the optimal conditions, the detection linear range of 350-1000 pg/mL and the detection limit of 0.24 ng/mL were provided by the proposed method. The relative standard deviations were less than 15% for both intra and inter-assay precision. This method has been successfully applied to determine ENR in spiked samples with the recovery of 103%-96%. It showed that CLEIA was a good potential method in the analysis of residues of veterinary drugs after treatment of related diseases.
authors.affiliation: College of Public Health, Zhengzhou University, Zhengzhou 450001, PR China.
authors.forename: Fei
authors.initials: F
authors.lastname: Yu
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Wu
authors.forename: Songcheng
authors.initials: S
authors.lastname: Yu
authors.forename: Huili
authors.initials: H
authors.lastname: Zhang
authors.forename: Hongquan
authors.initials: H
authors.lastname: Zhang
authors.forename: Lingbo
authors.initials: L
authors.lastname: Qu
authors.forename: Peter de B
authors.initials: Pde B
authors.lastname: Harrington
mesh_terms: D000218:Adamantane / Q000737:chemistry; D000469:Alkaline Phosphatase / Q000737:chemistry / Q000378:metabolism; D000970:Antineoplastic Agents / Q000032:analysis* / Q000378:metabolism; D002138:Calibration; D000077422:Enrofloxacin; D024841:Fluoroquinolones / Q000032:analysis* / Q000378:metabolism; D007124:Immunoenzyme Techniques / Q000191:economics / Q000379:methods*; D057230:Limit of Detection; D049449:Luminescence; D008163:Luminescent Measurements / Q000191:economics / Q000379:methods*; D013997:Time Factors
chemical_list: D000970:Antineoplastic Agents; D024841:Fluoroquinolones; D000077422:Enrofloxacin; D000469:Alkaline Phosphatase; D000218:Adamantane