title: In vitro and in vivo osteogenesis of human mesenchymal stem cells derived from skin, bone marrow and dental follicle tissues.
abstract: The present study evaluated the human mesenchymal stem cells (hMSCs) isolated from skin (hSMSC), bone marrow (hBMSC) and dental follicle (hDFMSC) tissues on their in vitro and in vivo osteogenic potential using demineralized bone matrix (DBM) and fibrin glue scaffold. Cells originated from three distinct tissues showed positive expressions of CD44, CD73, CD90, CD105 and vimentin, and differentiation ability into osteocytes, adipocytes and chondrocytes. hMSCs from all tissues co-cultured with a mixed DBM and fibrin glue scaffold in non-osteogenic induction media were positively stained by von Kossa and expressed osteoblast-related genes, such as osteocalcin (OC), osteonectin (ON), runt-related transcription factor 2 (Runx2) and osterix. For in vivo osteogenic evaluation, PKH26 labeled hMSCs were implanted into the subcutaneous spaces of athymic mice with a mixed scaffold. At 4 weeks of implantation, PKH26 labeled cells were detected in all hMSC-implanted groups. Bone formation with OC expression and radio-opacity intensity were observed around DBM scaffold in all hMSC-implanted groups. Interestingly, hDFMSCs-implanted group showed the highest OC expression and calcium content. These findings demonstrated that hDFMSCs could be a potential alternative autologous cell source for bone tissue engineering.
authors.affiliation: Department of Oral & Maxillofacial Surgery, School of Medicine and Institute of Health Science, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Bong-Wook
authors.initials: BW
authors.lastname: Park
authors.forename: Eun-Ju
authors.initials: EJ
authors.lastname: Kang
authors.forename: June-Ho
authors.initials: JH
authors.lastname: Byun
authors.forename: Myeong-Gyun
authors.initials: MG
authors.lastname: Son
authors.forename: Hyun-Joon
authors.initials: HJ
authors.lastname: Kim
authors.forename: Young-Sool
authors.initials: YS
authors.lastname: Hah
authors.forename: Tae-Ho
authors.initials: TH
authors.lastname: Kim
authors.forename: B
authors.initials: B
authors.lastname: Mohana Kumar
authors.forename: Sun-A
authors.initials: SA
authors.lastname: Ock
authors.forename: Gyu-Jin
authors.initials: GJ
authors.lastname: Rho
mesh_terms: D050156:Adipogenesis / Q000235:genetics*; D000818:Animals; D001853:Bone Marrow / Q000254:growth & development; D001857:Bone Matrix / Q000166:cytology; D002454:Cell Differentiation / Q000235:genetics*; D019070:Cell Lineage; D002478:Cells, Cultured; D020219:Chondrogenesis / Q000235:genetics*; D003795:Dental Sac / Q000166:cytology / Q000254:growth & development; D015718:Fibrin Tissue Adhesive / Q000494:pharmacology; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D009930:Organic Chemicals / Q000737:chemistry; D015675:Osteocalcin / Q000378:metabolism; D010012:Osteogenesis / Q000235:genetics; D015676:Osteonectin / Q000378:metabolism; D012867:Skin / Q000166:cytology / Q000254:growth & development
chemical_list: D015718:Fibrin Tissue Adhesive; D009930:Organic Chemicals; D015676:Osteonectin; C070080:PKH 26; D015675:Osteocalcin