title: Biochemical analysis and in vivo hypoglycemic activity of a grape polyphenol-soybean flour complex.
abstract: Defatted soybean flour (DSF) can efficiently sorb, concentrate, and stabilize polyphenols, but not sugars, from Concord grape juice, to yield grape polyphenol-enriched DSF. Sorption of grape polyphenols to DSF particles was dependent on the ratio of DSF and grape juice concentrate used, but not time of mixing or pH. Depending on ratios of starting materials, 1 g of grape polyphenol-enriched DSF contained 1.6-10.4 mg of anthocyanins, 7.5-93.1 mg of proanthocyanidins, and 20.5-144.5 mg of total polyphenols. LC-MS analysis of grape juice samples before and after addition and removal of DSF and eluate from grape polyphenol-enriched DSF confirmed that a broad range of grape compounds were sorbed to the DSF matrix. Finally, grape polyphenol-enriched DSF was able to significantly lower blood glucose levels in hyperglycemic C57BL/6J mice. The data indicate that grape polyphenol-enriched DSF can provide a high-protein, low-sugar ingredient for delivery of concentrated grape polyphenolics.
authors.affiliation: School of Environmental and Biological Sciences, Foran Hall, Rutgers, The State University of New Jersey, 59 Dudley Road, New Brunswick, New Jersey 08901, USA. roopchand@aesop.rutgers.edu
authors.forename: Diana E
authors.initials: DE
authors.lastname: Roopchand
authors.forename: Peter
authors.initials: P
authors.lastname: Kuhn
authors.forename: Alexander
authors.initials: A
authors.lastname: Poulev
authors.forename: Andrew
authors.initials: A
authors.lastname: Oren
authors.forename: Mary Ann
authors.initials: MA
authors.lastname: Lila
authors.forename: Bertold
authors.initials: B
authors.lastname: Fridlender
authors.forename: Ilya
authors.initials: I
authors.lastname: Raskin
mesh_terms: D000327:Adsorption; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D005433:Flour / Q000032:analysis*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006943:Hyperglycemia / Q000188:drug therapy* / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000008:administration & dosage* / Q000737:chemistry*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D059808:Polyphenols / Q000008:administration & dosage* / Q000737:chemistry*; D013025:Glycine max / Q000737:chemistry*; D027843:Vitis / Q000737:chemistry*
chemical_list: D001786:Blood Glucose; D007004:Hypoglycemic Agents; D059808:Polyphenols