title: In vitro cytotoxicity of Strobilanthes crispus ethanol extract on hormone dependent human breast adenocarcinoma MCF-7 cell.
abstract: BACKGROUND
  Strobilanthes crispus has been traditionally used as antidiabetic, anticancer, diuretic, antilytic and laxative agent. However, cytotoxicity and antiproliferative effect of S. crispus is still unclear.
  
  
  RESULTS
  Strobilanthes cripus was able to reduce cell viability and proliferation in MTT and BrdU assays. Both cell cycle progression and Tunel assay suggested that IC50 of S. crispus ethanol extract induced sub-G1 cell cycle phase, and DNA fragmentation. On the other hand, translocation of mitochondria cytochrome c release, induction of caspase 3/7 and p53 while suppress XIAP on treated MCF-7 cell were also observed in this study.
  
  
  CONCLUSIONS
  Our findings suggest that S. crispus ethanol extract induced apoptosis and DNA fragmentation on hormone dependent breast cancer cell line MCF-7 via mitochondria dependent p53 apoptosis pathway.
authors.affiliation: Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia. meganjournal@gmail.com
authors.forename: Hueh Zan
authors.initials: HZ
authors.lastname: Chong
authors.forename: Asmah
authors.initials: A
authors.lastname: Rahmat
authors.forename: Swee Keong
authors.initials: SK
authors.lastname: Yeap
authors.forename: Abdah
authors.initials: A
authors.lastname: Md Akim
authors.forename: Noorjahan Banu
authors.initials: NB
authors.lastname: Alitheen
authors.forename: Fauziah
authors.initials: F
authors.lastname: Othman
authors.forename: Cheng Lian
authors.initials: CL
authors.lastname: Gwendoline-Ee
mesh_terms: D027901:Acanthaceae; D000230:Adenocarcinoma / Q000188:drug therapy* / Q000378:metabolism; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology / Q000627:therapeutic use*; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D053179:Caspase 7 / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D045304:Cytochromes c / Q000378:metabolism; D053938:DNA Fragmentation; D005260:Female; D016193:G1 Phase / Q000187:drug effects; D006728:Hormones / Q000378:metabolism; D006801:Humans; D020128:Inhibitory Concentration 50; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D051636:X-Linked Inhibitor of Apoptosis Protein / Q000378:metabolism
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D006728:Hormones; D010936:Plant Extracts; D016159:Tumor Suppressor Protein p53; D051636:X-Linked Inhibitor of Apoptosis Protein; C496866:XIAP protein, human; D045304:Cytochromes c; D053148:Caspase 3; D053179:Caspase 7