title: Determination of natural pesticides in fresh fruits using liquid chromatography/mass spectrometry.
abstract: An analytical procedure was developed for the determination of some natural pesticides (piperonyl butoxide, nicotine, rotenone, spinosad, and abamectin B1a) in fruit matrixes. The quick, easy, cheap, effective, rugged, and safe (QuEChERS) method was used for extraction. Analysis of the extract was performed by LC-electrospray ionization (ESI)-MS/MS. The ions prominent in the ESI spectra were [M+Na]+ for abamectin B1a, [M+NH4]+ for piperonyl butoxide, and [M+H]+ for the rest of the compounds. A Zorbax SB-C18 column was used with a programmed gradient mobile phase consisting of (A) water containing 0.1% formic acid and 5 mM ammonium formate, and (B) acetonitrile containing 2 mM sodium acetate. The method was linear within the investigated concentration range, displaying a calibration curve correlation factor of 0.99. The CVs obtained were below 20%, and recoveries were in the 70-110% range.
authors.affiliation: University of Murcia, Faculty of Chemistry, Department of Agricultural Chemistry, Geology and Pedology, Campus Espinardo, s/n, 30100 Murcia, Spain. irene_ra@hotmail.com
authors.forename: Irene
authors.initials: I
authors.lastname: Ruiz
authors.forename: Ascensión
authors.initials: A
authors.lastname: Morales
authors.forename: Alberto
authors.initials: A
authors.lastname: Barba
authors.forename: José
authors.initials: J
authors.lastname: Oliva
mesh_terms: D001688:Biological Products / Q000032:analysis* / Q000302:isolation & purification; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002957:Citrus / Q000737:chemistry; D005638:Fruit / Q000737:chemistry*; D007202:Indicators and Reagents; D057230:Limit of Detection; D010573:Pesticide Residues / Q000032:analysis; D010575:Pesticides / Q000032:analysis* / Q000302:isolation & purification; D027861:Prunus / Q000737:chemistry; D012015:Reference Standards; D012996:Solutions; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D027843:Vitis / Q000737:chemistry
chemical_list: D001688:Biological Products; D007202:Indicators and Reagents; D010573:Pesticide Residues; D010575:Pesticides; D012996:Solutions