title: Enantioselective and label-free detection of oligopeptide via fluorescent indicator displacement.
abstract: In this work, a simple and label-free fluorescent method via fluorescent indicator displacement (FID) was proposed for enantioselectively determining d-enantiomer of arginine vasopressin (DV) using DV-specific DNA aptamer (V-apt) and one guanidiniophthalocyanine dye (Zn-DIGP). Zn-DIGP that preferentially binds to single-stranded DNA with fluorescence enhancement rather than duplexes occupies the long internal loop of V-apt and generates intensive fluorescence. Then DV is introduced into the solution containing Zn-DIGP and V-apt, and displaces the Zn-DIGP from the binding site of internal loop, leading to fluorescence decrease. But l-enantiomer cannot induce any fluorescence change due to the selectivity of V-apt. This established FID technique can detect DV with a detection limit of 100 nM and exhibits a broad linear range, and is able to discriminate enantiomers of arginine vasopressin unambiguously. Moreover chiral separation by chromatography, complicated experimental procedures and covalent modification of tags (such as organic dyes, redox-active metal complexes) are avoided in our strategy. This simple and label-free method is promising for fabricating diverse aptasensors to determine other biomolecules and drugs.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China; Graduate School of the Chinese Academy of Sciences, Beijing 100039, China.
authors.forename: Jiangtao
authors.initials: J
authors.lastname: Ren
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China. Electronic address: jhwang@ciac.jl.cn.
authors.forename: Jiahai
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China; Department of Chemistry, Physics and Applied Mathematics, State University of New York at Stony Brook, Stony Brook, NY 11794, USA. Electronic address: jin.wang.1@stonybrook.edu.
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.affiliation: Institute of Organic Chemistry, University of Zürich, Winterthurerstrasse 190, Zürich CH-8057, Switzerland, United Kingdom.
authors.forename: Nathan W
authors.initials: NW
authors.lastname: Luedtke
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China. Electronic address: ekwang@ciac.jl.cn.
authors.forename: Erkang
authors.initials: E
authors.lastname: Wang
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry; D001127:Arginine Vasopressin / Q000032:analysis / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D002412:Cations; D004277:DNA, Single-Stranded / Q000737:chemistry; D005456:Fluorescent Dyes; D007211:Indoles; D054833:Isoindoles; D009842:Oligopeptides / Q000032:analysis* / Q000737:chemistry; D009942:Organometallic Compounds; D013237:Stereoisomerism; D017967:Zinc Compounds
chemical_list: D052157:Aptamers, Nucleotide; D002412:Cations; D004277:DNA, Single-Stranded; D005456:Fluorescent Dyes; D007211:Indoles; D054833:Isoindoles; D009842:Oligopeptides; D009942:Organometallic Compounds; D017967:Zinc Compounds; D001127:Arginine Vasopressin; C052159:Zn(II)-phthalocyanine