title: Ultra sensitive measurement of endogenous epinephrine and norepinephrine in human plasma by semi-automated SPE-LC-MS/MS.
abstract: Measurement of endogenous epinephrine (E) and norepinephrine (NE) in human plasma is very challenging due to lower endogenous concentrations as compared with animal plasma. An LC-MS/MS in combination with alumina-based SPE and derivatization procedure was validated for the measurement of E and NE in human plasma with acceptable intra-day and inter-day accuracy and precision. Sample was extracted with semi-automated alumina 96-well solid phase extraction (SPE) cartridge. The resulting eluent was dried and derivatized using d4-acetaldehyde. The analytes were separated on a monolithic C(18) column. Extraction efficiencies were >66% for E and NE. The lower limit of quantitation (LLOQ) was 5.00 pg/mL for E and 20.0 pg/mL for NE.
authors.affiliation: Biomarker Research, PDM, PGRD, Pfizer Inc., Groton, CT 06340, USA.
authors.forename: Guodong
authors.initials: G
authors.lastname: Zhang
authors.forename: Yizhong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Chengjie
authors.initials: C
authors.lastname: Ji
authors.forename: Thomas
authors.initials: T
authors.lastname: McDonald
authors.forename: Justin
authors.initials: J
authors.lastname: Walton
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Groeber
authors.forename: Rick C
authors.initials: RC
authors.lastname: Steenwyk
authors.forename: Zhaosheng
authors.initials: Z
authors.lastname: Lin
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D004837:Epinephrine / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D057230:Limit of Detection; D009638:Norepinephrine / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D009638:Norepinephrine; D004837:Epinephrine