title: Effective induction of oral anaphylaxis to ovalbumin in mice sensitized by feeding of the antigen with aid of oil emulsion and salicylate.
abstract: It is important to evaluate the ability of novel proteins in food crops and products to elicit potentially harmful immunologic responses, including allergic hypersensitivity. We developed a novel mouse model of food allergy involving an oral challenge of a protein antigen after feeding of the antigen in combination with modulating factors often ingested in daily life, namely, dietary oil emulsion and salicylate. In the model, BALB/c mice were sensitized orally for three weeks with ovalbumin (OVA) in linoleic acid/lecithin emulsion, followed immediately by intraperitoneal injection of sodium salicylate. At the end of the sensitization, the incidence of mice positive for serum OVA-specific IgG1 but not IgE had significantly increased in the combined-sensitization group. After the 3-week sensitization, a single or double oral challenge with OVA effectively and significantly caused severe anaphylaxis, as compared with the groups sensitized with OVA in the emulsion or the vehicle alone. Moderate increase of plasma histamine and intestinal abnormality in histology was found only in the combined-sensitization group. Anaphylaxis symptoms in the sensitized mice were induced more by oral challenge than by intravenous challenge, suggesting a critical role for the mucosal system. This is the first model for successful induction of oral anaphylaxis in mice sensitized by feeding of food protein without adjuvant. It will be useful to elucidate the mechanism of food allergy and to detect modulating factors of oral allergy at sensitization using this model, which simulates real life conditions.
authors.affiliation: Hatano Research Institute, Food and Drug Safety Center, Kanagawa, Japan. shindo.t@fdsc.or.jp
authors.forename: Tomoko
authors.initials: T
authors.lastname: Shindo
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Kanazawa
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Saito
authors.forename: Kohichi
authors.initials: K
authors.lastname: Kojima
authors.forename: Motoyasu
authors.initials: M
authors.lastname: Ohsawa
authors.forename: Reiko
authors.initials: R
authors.lastname: Teshima
mesh_terms: D000284:Administration, Oral; D000485:Allergens / Q000008:administration & dosage*; D000707:Anaphylaxis / Q000097:blood / Q000209:etiology* / Q000473:pathology; D000818:Animals; D004195:Disease Models, Animal; D004655:Emulsions; D005260:Female; D005512:Food Hypersensitivity / Q000097:blood / Q000209:etiology* / Q000473:pathology; D007074:Immunoglobulin G / Q000097:blood; D007421:Intestine, Small / Q000187:drug effects / Q000473:pathology; D054709:Lecithins / Q000008:administration & dosage; D019787:Linoleic Acid / Q000008:administration & dosage; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000008:administration & dosage*; D020156:Salicylic Acid / Q000008:administration & dosage
chemical_list: D000485:Allergens; D004655:Emulsions; D007074:Immunoglobulin G; D054709:Lecithins; D010047:Ovalbumin; D019787:Linoleic Acid; D020156:Salicylic Acid