title: Near infrared spectroscopy and multivariate calibration for simultaneous determination of glucose, triglycerides and high-density lipoprotein in animal plasma.
abstract: The quantitative analysis of glucose, triglycerides and high-density lipoprotein (HDL) in rat plasma without sample pre-treatment using direct near-infrared spectroscopy was studied. Comparison was made of several multivariate calibration techniques and algorithms for data pre-processing and variable selection, including partial least squares (PLS), interval partial least squares (iPLS), genetic algorithm (GA) and successive projections algorithm (SPA). Variable selection yielded good results for the correlation coefficient and Root Mean Square Error of Prediction (RMSEP) values for the three parameters, especially triglycerides. The RMSEP values for glucose, triglycerides and HDL produced by the PLS model were 6.08, 16.07 and 2.03 mg dl(-1), respectively. F tests and t-tests were performed to compare the results of the models with each other and with a reference method. These results suggests that the PLS method can be used to simultaneously determine the concentrations of glucose, triglycerides and HDL in complicated biological fluids with NIR spectroscopy, offering an alternative analysis in animals.
authors.affiliation: Universidade Federal do Rio Grande do Norte, Instituto de Química, Grupo de Pesquisa em Quimiometria Aplicada, CEP 59072-970 Natal, RN, Brazil.
authors.forename: Ana Carolina de Oliveira
authors.initials: AC
authors.lastname: Neves
authors.forename: Aurigena Antunes
authors.initials: AA
authors.lastname: de Araújo
authors.forename: Bruna Laís
authors.initials: BL
authors.lastname: Silva
authors.forename: Patrícia
authors.initials: P
authors.lastname: Valderrama
authors.forename: Paulo Henrique
authors.initials: PH
authors.lastname: Março
authors.forename: Kássio Michell Gomes
authors.initials: KM
authors.lastname: de Lima
mesh_terms: D000465:Algorithms; D000818:Animals; D001786:Blood Glucose / Q000032:analysis*; D002138:Calibration; D008076:Cholesterol, HDL / Q000097:blood*; D016018:Least-Squares Analysis; D008297:Male; D051381:Rats; D019265:Spectroscopy, Near-Infrared / Q000379:methods*; D014280:Triglycerides / Q000097:blood*
chemical_list: D001786:Blood Glucose; D008076:Cholesterol, HDL; D014280:Triglycerides