title: Three-dimensional differential interference contrast microscopy using synthetic aperture imaging.
abstract: We implement differential interference contrast (DIC) microscopy using high-speed synthetic aperture imaging that expands the passband of coherent imaging by a factor of 2.2. For an aperture synthesized coherent image, we apply for the numerical post-processing and obtain a high-contrast DIC image for arbitrary shearing direction and bias retardation. In addition, we obtain images at different depths without a scanning objective lens by numerically propagating the acquired coherent images. Our method achieves high-resolution and high-contrast 3-D DIC imaging of live biological cells. The proposed method will be useful for monitoring 3-D dynamics of intracellular particles.
authors.affiliation: Korea University, Department of Physics, Seoul 136-701, Republic of Korea.
authors.forename: Moonseok
authors.initials: M
authors.lastname: Kim
authors.forename: Youngwoon
authors.initials: Y
authors.lastname: Choi
authors.forename: Christopher
authors.initials: C
authors.lastname: Fang-Yen
authors.forename: Yongjin
authors.initials: Y
authors.lastname: Sung
authors.forename: Kwanhyung
authors.initials: K
authors.lastname: Kim
authors.forename: Ramachandra R
authors.initials: RR
authors.lastname: Dasari
authors.forename: Michael S
authors.initials: MS
authors.lastname: Feld
authors.forename: Wonshik
authors.initials: W
authors.lastname: Choi
mesh_terms: D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D021621:Imaging, Three-Dimensional / Q000295:instrumentation*; D007909:Lenses; D008858:Microscopy, Phase-Contrast / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity