title: Autophagy in the intestinal epithelium regulates Citrobacter rodentium infection.
abstract: Autophagy, a ubiquitous degradation pathway, is important for the survival and homeostasis of cells. Previous studies have demonstrated the role of autophagy in host defense against bacterial infection, but the importance of autophagy in the intestinal epithelium for the regulation of bacterial infection has not been fully elucidated. In this study, we showed that the essential autophagy protein Atg7 is required for resistance to Citrobacter rodentium infection in the intestinal epithelium. Infected mice in which Atg7 had been conditionally deleted from the intestinal epithelium exhibited greater clinical evidence of disease and higher expression levels of pro-inflammatory cytokine mRNA in the large intestine. Moreover, C. rodentium clearance was reduced in the Atg7 conditional knockout mice. These results demonstrate that autophagy in intestinal epithelial cells plays an important role in host defense against C. rodentium infection and the regulation of C. rodentium infectious colitis.
authors.affiliation: Division of Gastroenterology, Department of Internal Medicine, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chu-o-ku, Kobe, Hyogo 650-0017, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Inoue
authors.forename: Shin
authors.initials: S
authors.lastname: Nishiumi
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Fujishima
authors.forename: Atsuhiro
authors.initials: A
authors.lastname: Masuda
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Shiomi
authors.forename: Koji
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Masayuki
authors.initials: M
authors.lastname: Nishida
authors.forename: Takeshi
authors.initials: T
authors.lastname: Azuma
authors.forename: Masaru
authors.initials: M
authors.lastname: Yoshida
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001343:Autophagy; D000071193:Autophagy-Related Protein 7; D001483:Base Sequence; D044082:Citrobacter rodentium / Q000276:immunology / Q000472:pathogenicity; D003092:Colitis / Q000235:genetics / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D016207:Cytokines / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D004756:Enterobacteriaceae Infections / Q000235:genetics / Q000276:immunology / Q000382:microbiology* / Q000473:pathology*; D007074:Immunoglobulin G / Q000097:blood; D007413:Intestinal Mucosa / Q000276:immunology / Q000382:microbiology* / Q000473:pathology*; D051379:Mice; D018345:Mice, Knockout; D008869:Microtubule-Associated Proteins / Q000172:deficiency / Q000235:genetics / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D000907:Antibodies, Bacterial; C502661:Atg7 protein, mouse; D016207:Cytokines; D017931:DNA Primers; D007074:Immunoglobulin G; D008869:Microtubule-Associated Proteins; D012333:RNA, Messenger; D000071193:Autophagy-Related Protein 7