title: Validation of Eupatorium triplinerve Vahl leaves, a skin care herb from East Kalimantan, using a melanin biosynthesis assay.
abstract: In searching for a new material made from natural resources that could be used as a whitening agent, we focused on the plants used for skin treatment by the native people of East Kalimantan. The methanol extract of the leaves of Eupatorium triplinerve Vahl showed antimelanogenesis activity in a melanin biosynthesis assay. By activity-guided fractionation, 7-methoxycoumarin (1) was isolated as an active compound. The IC50 of 1 on mushroom tyrosinase was 2360 μM (L-tyrosine was used as the substrate) and above 2840 μM (L-DOPA was used as the substrate), respectively. Regarding melanin formation inhibition in B16 melanoma cells, the IC50 of 1 was 1780 μM with 83% cell viability at IC50. Based on these results, we validated that the leaf extract is in line with the traditional use of the Dayak tribe in East Kalimantan.
authors.affiliation: Department of Forest Products Technology, Faculty of Forestry, Mulawarman University, Samarinda, Indonesia.
authors.forename: Enos Tangke
authors.initials: ET
authors.lastname: Arung
authors.forename: Harlinda
authors.initials: H
authors.lastname: Kuspradini
authors.forename: Irawan Wijaya
authors.initials: IW
authors.lastname: Kusuma
authors.forename: Kuniyoshi
authors.initials: K
authors.lastname: Shimizu
authors.forename: Ryuichiro
authors.initials: R
authors.lastname: Kondo
mesh_terms: D057886:Bleaching Agents / Q000494:pharmacology*; D001893:Borneo; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003374:Coumarins / Q000302:isolation & purification / Q000494:pharmacology*; D036602:Eupatorium / Q000737:chemistry*; D006801:Humans; D020128:Inhibitory Concentration 50; D007980:Levodopa / Q000037:antagonists & inhibitors; D008543:Melanins / Q000096:biosynthesis*; D008546:Melanoma, Experimental; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves; D017592:Skin Care
chemical_list: D057886:Bleaching Agents; D003374:Coumarins; D008543:Melanins; D010936:Plant Extracts; D007980:Levodopa; D014442:Monophenol Monooxygenase