title: The stimulation of adipose-derived stem cell differentiation and mineralization by ordered rod-like fluorapatite coatings.
abstract: In this study, the effect of ordered rod-like FA coatings of metal discs on adipose-derived stem cell (ASC)'s growth, differentiation and mineralization was studied in vitro; and their mineral inductive effects in vivo. After 3 and 7 days, the cell number on the metal surfaces was significantly higher than those on the ordered and disordered FA surfaces. However, after 4 weeks much greater amounts of mineral formation was induced on the two FA surfaces with and even without osteogenesis induction. The osteogenic profiles showed the up regulation of a set of pro-osteogenic transcripts and bone mineralization phenotypic markers when the ASCs were grown on FA surfaces compared to metal surfaces at 7 and 21 days. In addition to BMP and TGFβ signaling pathways, EGF and FGF pathways also appeared to be involved in ASC differentiation and mineralization. In vivo studies showed accelerated and enhanced mineralized tissue formation integrated within ordered FA coatings. After 5 weeks, over 80% of the ordered FA coating was integrated with a mineralized tissue layer covering the implants. Both the intrinsic properties of the FA crystals and the topography of the FA coating appeared to dominate the cell differentiation and mineralization process.
authors.affiliation: Department of Cariology, Restorative Sciences and Endodontics, Dental School, University of Michigan, Ann Arbor, MI 48109, USA. junlc@umich.edu
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Wang
authors.forename: Qiming
authors.initials: Q
authors.lastname: Jin
authors.forename: Taocong
authors.initials: T
authors.lastname: Jin
authors.forename: Syweren
authors.initials: S
authors.lastname: Chang
authors.forename: Zhaocheng
authors.initials: Z
authors.lastname: Zhang
authors.forename: Agata
authors.initials: A
authors.lastname: Czajka-Jakubowska
authors.forename: William V
authors.initials: WV
authors.lastname: Giannobile
authors.forename: Jacques E
authors.initials: JE
authors.lastname: Nör
authors.forename: Brian H
authors.initials: BH
authors.lastname: Clarkson
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D001031:Apatites; D015153:Blotting, Western; D002113:Calcification, Physiologic; D002448:Cell Adhesion; D002454:Cell Differentiation; D006133:Growth Substances / Q000378:metabolism; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis; D016133:Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: D001031:Apatites; D006133:Growth Substances; C025105:fluorapatite