title: Neurosecretory cell-based biosensor: monitoring secretion of adrenal chromaffin cells by local extracellular acidification using light-addressable potentiometric sensor.
abstract: Vesicular exocytosis plays an important role in many physiological processes. The dense-core vesicles release of chromafﬁn cells is a suitable model for the presynaptic process in neurosecretory cells. In this study, light-addressable potentiometric sensor (LAPS) was introduced as a label-free recording method for vesicle release by the local extracellular acidification. The chromafﬁn cells are directly cultured on the sensor surface. After cells and LAPS hybrid system is established, the events of vesicular exocytosis are recorded. Protons stored in the vesicles and co-released with transmitters, induced a brief acidic shifts in the cell-sensor cleft. Under the stimulation of the KCl and acetylcholine (Ach), the signals presented the different amplitude and exocytosis rate, and reflected the specific features of the exocytosis. The result indicates that neurosecretory cell-based biosensor will provide a useful platform for neurosecretion mechanism research by monitoring the exocytotic activities with extracellular acidification sensing.
authors.affiliation: Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, PR China; State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Shanghai 200050, PR China.
authors.forename: Qingjun
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, PR China.
authors.forename: Ning
authors.initials: N
authors.lastname: Hu
authors.affiliation: Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, PR China.
authors.forename: Fenni
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, PR China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.affiliation: Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, PR China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Ye
authors.affiliation: Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, PR China; State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Shanghai 200050, PR China. Electronic address: cnpwang@zju.edu.cn.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D018914:Cells, Immobilized; D019439:Chromaffin Cells / Q000187:drug effects / Q000378:metabolism*; D004867:Equipment Design; D005089:Exocytosis / Q000187:drug effects; D045604:Extracellular Fluid / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D009489:Neurosecretion / Q000187:drug effects; D018377:Neurotransmitter Agents / Q000378:metabolism; D011189:Potassium Chloride / Q000494:pharmacology; D011199:Potentiometry / Q000295:instrumentation / Q000379:methods
chemical_list: D018377:Neurotransmitter Agents; D011189:Potassium Chloride; D000109:Acetylcholine