title: Therapeutic effects of novel resin bonding systems containing bioactive glasses on mineral-depleted areas within the bonded-dentine interface.
abstract: This study aimed in evaluating the effects of two experimental resin bonding systems containing conventional Bioglass 45S5 (BAG) or Zinc-polycarboxylated bioactive glass (BAG-Zn) micro-fillers on the resin-bonded dentine interface after storage in a simulated body fluid solution (SBFS). Three resin bonding systems were formulated: Resin-A: (BAG containing); Resin-B; (BAG-Zn containing); Resin-C (no filler). The ability of the experimental resins to evoke apatite formation was evaluated using confocal Raman spectroscopy. Acid-etched dentine specimens were bonded, and prepared for AFM/nano-indentation analysis in a fully-hydrated status to evaluate the modulus of elasticity (Ei) and hardness (Hi) across the interface at different SBFS storage periods. Further resin-dentine specimens were tested for microtensile bond strength after 24 h or 3 months of SBFS storage. SEM examination was performed after de-bonding and confocal laser microscopy was used to evaluate the ultramorphology of the interfaces and micropermeability. The resin A and B showed a consistent presence of apatite (967 cm(-1)), reduced micropermeability within the resin-dentine interface and a significant increase of the Ei and Hi along the bonded-dentine interface after prolonged SBFS storage. Bond strength values were affected by the resin system (P < 0.0001) and by storage time (P < 0.0001) both after 24 h and 3 months of SBFS storage. In conclusion, resin bonding systems containing bioactive fillers may a have therapeutic effect on the nano-mechanical properties and sealing ability of mineral-depleted resin-dentine interface.
authors.affiliation: Biomaterials, Biomimetics and Biophotonics, King's College London Dental Institute, Guy's Dental Hospital, London, England, UK. salvatore.sauro@kcl.ac.uk
authors.forename: Salvatore
authors.initials: S
authors.lastname: Sauro
authors.forename: Raquel
authors.initials: R
authors.lastname: Osorio
authors.forename: Timothy F
authors.initials: TF
authors.lastname: Watson
authors.forename: Manuel
authors.initials: M
authors.lastname: Toledano
mesh_terms: D001696:Biomechanical Phenomena; D002516:Ceramics / Q000737:chemistry / Q000627:therapeutic use*; D001840:Dental Bonding / Q000379:methods*; D003804:Dentin / Q000737:chemistry / Q000187:drug effects; D017220:Dentin-Bonding Agents / Q000737:chemistry / Q000627:therapeutic use; D005898:Glass / Q000737:chemistry; D006801:Humans; D066298:In Vitro Techniques; D008422:Materials Testing; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D012117:Resins, Synthetic / Q000737:chemistry / Q000627:therapeutic use; D013059:Spectrum Analysis, Raman; D013499:Surface Properties; D013718:Tensile Strength; D017001:Tooth Demineralization / Q000628:therapy; D015032:Zinc / Q000737:chemistry
chemical_list: D017220:Dentin-Bonding Agents; D012117:Resins, Synthetic; C425330:bioactive glass 45S5; D015032:Zinc