title: Rapid quantitative analysis of clarithromycin in rat plasma by UPLC-MS/MS after intravenous injection of the clarithromycin-loaded ultrafine PLGA nanoparticles.
abstract: Nanoparticles were designed to encapsulate drugs to alter their pharmacological behaviors, therefore, it is very essential to monitor the pharmacokinetic profile of drug encapsulated in nanoparticles in order to clarify and predict their efficacy and side effects. In this paper, we reported a simple, rapid μ-elution 96-well solid phase extraction (μSPE) method combining with ultra high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for determination of nanoformulated drug in rat plasma. This method presented satisfactory results in terms of sensitivity, precision, accuracy, and recovery, for the first time, of quantitatively analyzing clarithromycin (CLA) in rat plasma after intravenous administration CLA-loaded ultrafine PLGA nanoparticles for pharmacokinetic study. This method has been proved to be fast, reliable and reproducible to accurately analyze drug encapsulated in polymeric nanoparticles sample for a pharmacokinetic study.
authors.affiliation: Center for Nanomedicine Research, National Health Research Institutes, Zhunan, Miaoli 350, Taiwan.
authors.forename: Yu-Jing
authors.initials: YJ
authors.lastname: Wang
authors.forename: Yi-Ting
authors.initials: YT
authors.lastname: Wu
authors.forename: Jia-Yi
authors.initials: JY
authors.lastname: Lin
authors.forename: Chih-Hung
authors.initials: CH
authors.lastname: Chu
authors.forename: Hsin-Ying
authors.initials: HY
authors.lastname: Huang
authors.forename: Yu-Chao
authors.initials: YC
authors.lastname: Wang
authors.forename: Jen-Kun
authors.initials: JK
authors.lastname: Chen
authors.forename: Chung-Shi
authors.initials: CS
authors.lastname: Yang
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D017291:Clarithromycin / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D007275:Injections, Intravenous; D019344:Lactic Acid / Q000008:administration & dosage* / Q000737:chemistry; D016014:Linear Models; D008297:Male; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D011100:Polyglycolic Acid / Q000008:administration & dosage* / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D052617:Solid Phase Microextraction; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D017291:Clarithromycin