title: Functional analysis of mouse ficolin-B and detection in neutrophils.
abstract: Ficolins and mannan-binding lectin recognize pathogen-associated molecular patterns and initiate the lectin pathway of complement activation via the associated serine proteases. In contrast to human ficolins and mouse ficolin-A, mouse ficolin-B has been considered incapable of complement activation. Dose-dependent binding of recombinant ficolin-B to immobilized GlcNAc, acetylated BSA, acetylated LDL, and fetuin was detected with ficolin-B-specific monoclonal antibodies. Recombinant ficolin-B bound to immobilized acetylated bovine serum albumin interacted with recombinant human mannan-binding lectin-associated serine protease-2, which led to C4 cleavage, thus demonstrating the capability of ficolin-B to activate the lectin pathway. Ficolin-B-specific monoclonal antibodies identified natural ficolin-B protein in lysates of mouse granulocytes isolated from the bone marrow. These results identify mouse ficolin-B as a functional member of the ficolin family activating complement via the lectin pathway.
authors.affiliation: Institute of Immunology, University of Regensburg, Germany.
authors.forename: Katja
authors.initials: K
authors.lastname: Hunold
authors.forename: Dorothea
authors.initials: D
authors.lastname: Weber-Steffens
authors.forename: Valeria L
authors.initials: VL
authors.lastname: Runza
authors.forename: Jens C
authors.initials: JC
authors.lastname: Jensenius
authors.forename: Daniela N
authors.initials: DN
authors.lastname: Männel
mesh_terms: D000818:Animals; D002460:Cell Line; D003167:Complement Activation / Q000276:immunology; D003181:Complement C4 / Q000276:immunology / Q000378:metabolism; D003165:Complement System Proteins / Q000276:immunology; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D050606:Mannose-Binding Protein-Associated Serine Proteases / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009504:Neutrophils / Q000276:immunology* / Q000378:metabolism*; D011485:Protein Binding / Q000276:immunology; D011994:Recombinant Proteins / Q000378:metabolism; D000097604:Ficolins
chemical_list: D003181:Complement C4; D003165:Complement System Proteins; D037102:Lectins; D050606:Mannose-Binding Protein-Associated Serine Proteases; D011994:Recombinant Proteins; D000097604:Ficolins; C105296:MASP2 protein, human