title: Structure of the O-antigen polysaccharide present in the lipopolysaccharide of  Cronobacter dublinensis  (subspecies lactaridi or lausannensis) HPB 3169.
abstract: Cronobacter dublinensis (formerly Enterobacter sakazakii) HPB 3169 is a pathogenic Gram-negative bacterium that produces a smooth-type lipopolysaccharide in which the antigenic O-polysaccharide component was determined to be a repeating pentasaccharide unit composed of L-rhamnose; 2-acetamido-2-deoxy-D-glucose; 3,6-dideoxy-3-(R)-3-hydroxybutyramido-D-glucose; and 3-deoxy-manno-oct-2-ulosonic acid in the respective molar ratio 2:1:1:1. Chemical and 2D NMR analyses of the O-polysaccharide and a pentasaccharide derived by the mild acid hydrolysis of the ketosyl linkage of the Kdo (3-deoxy-D-manno-2-octulosonic acid) residue in the O-polysaccharide established that the O-antigen is a high molecular mass unbranched polymer of a repeating pentasaccharide unit and has the structure [see formula in text] where Bu is a (R)-3-hydroxybutanoyl substituent. The O-antigen is structurally similar to that of the recently reported Cronobacter sakazakii strain G706 (designated as serotype O5), except that in strain G706 the d-Qui3N is in its N-acetyl form, in contrast to its presence as a 3-deoxy-3-(R)-3-hydroxybutyramido derivative in the C. sakazakii HPB 3169 strain O-antigen.
authors.affiliation: Institute for Biological Sciences, National Research Council, Ottawa, ON K1A 0R6, Canada.
authors.forename: Leann L
authors.initials: LL
authors.lastname: MacLean
authors.forename: Evgeny
authors.initials: E
authors.lastname: Vinogradov
authors.forename: Franco
authors.initials: F
authors.lastname: Pagotto
authors.forename: Malcolm B
authors.initials: MB
authors.lastname: Perry
mesh_terms: D000117:Acetylglucosamine / Q000378:metabolism; D000595:Amino Acid Sequence; D002240:Carbohydrate Sequence; D059126:Cronobacter / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D019081:O Antigens / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry
chemical_list: D008070:Lipopolysaccharides; D019081:O Antigens; D009844:Oligosaccharides; D005947:Glucose; D000117:Acetylglucosamine