title: Isoforms of secretory group two phospholipase A (sPLA2) in mouse ocular surface epithelia and lacrimal glands.
abstract: OBJECTIVE
  To compare and contrast the distribution patterns of select secretory group two phospholipase A (sPLA2) isoforms in corneal epithelia (CN), conjunctival epithelia (CNJ), and lacrimal glands (LG) of BALB/c and C57BL/6 mice.
  
  
  METHODS
  Gene expression of select sPLA2 isoforms was quantified via real-time reverse-transcription PCR (qRT(2)-PCR). Immunofluorescence assay (IFA) of the sPLA2-IIa, -V, and -X isoforms were used to confirm qRT(2)-PCR results. sPLA2-IIa function was confirmed via in vitro CN and CNJ culturing.
  
  
  RESULTS
  qRT(2)-PCR revealed that sPLA2 isoforms (pla2g5, 12a, and 12b), cPLA2 isoform (pla2g4a), iPLA2 isoform (pla2g6), and PLA2-receptor (pla2r1) were present in all tissues of both strains, whereas sPLA2 isoforms (pla2g1b, 2e, and 3) were absent. sPLA2 isoforms (pla2g2a, 2d, 2f, and 10) showed tissue- and strain-specific expression: 2a in BALB/c CNJ only; 2d at higher levels in CNJ than LG; and 2f and 10 in CN and CNJ, but absent in LG. Upon dry eye (DE) induction, pla2g2a, 2d, and 2f were upregulated in BALB/c CNJ, and 10 was absent from CN. Furthermore, BALB/c DE mice showed upregulation of pla2r1 in CN and CNJ and downregulation of 12a and 12b in LG. IFA of sPLA2-IIa, -V, and -X in DE CNJ confirmed the upregulation of pla2g2a, 5, and 10. Last, in vitro CN and CNJ culturing confirmed that sPLA2-IIa amplifies ocular surface inflammation in CNJ but not in CN.
  
  
  CONCLUSIONS
  sPLA2 isoforms exhibit differential expression patterns when comparing BALB/c with C57BL/6 mice; and DE with control BALB/c mice. These findings suggest that at least some sPLA2 isoforms must have significant roles in ocular surface physiology and inflammation.
authors.affiliation: Department of Ophthalmology, Mount Sinai School of Medicine, New York, NY 10029, USA. yi.wei@mssm.edu
authors.forename: Yi
authors.initials: Y
authors.lastname: Wei
authors.forename: Alexander
authors.initials: A
authors.lastname: Pinhas
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Seth
authors.initials: S
authors.lastname: Epstein
authors.forename: Ju
authors.initials: J
authors.lastname: Wang
authors.forename: Penny
authors.initials: P
authors.lastname: Asbell
mesh_terms: D000818:Animals; D003228:Conjunctiva / Q000378:metabolism; D019573:Epithelium, Corneal / Q000201:enzymology*; D005455:Fluorescent Antibody Technique; D007249:Inflammation / Q000201:enzymology; D007527:Isoenzymes / Q000378:metabolism; D007765:Lacrimal Apparatus / Q000201:enzymology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D054497:Phospholipases A2, Secretory / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D007527:Isoenzymes; D054497:Phospholipases A2, Secretory