title: Imaging protein interactions by FRET microscopy: labeling proteins with fluorescent dyes.
abstract: This protocol provides a method for labeling proteins, such as antibodies and purified recombinant proteins, with succinimide esters of sulfoindocyanine (Cy) dyes. Cy dyes covalently bind to free amino groups (the α-amino-terminal or ε-amino groups on lysine side chains).
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Verveer
authors.forename: Oliver
authors.initials: O
authors.lastname: Rocks
authors.forename: Ailsa G
authors.initials: AG
authors.lastname: Harpur
authors.forename: Philippe I H
authors.initials: PI
authors.lastname: Bastiaens