title: Overexpression of CDC20 predicts poor prognosis in primary non-small cell lung cancer patients.
abstract: BACKGROUND
  This study examined the expression of CDC20 in human non-small cell lung cancer (NSCLC), explored its clinicopathological significance, and evaluated as a potential prognostic marker.
  
  
  METHODS
  A total of 362 cases of NSCLCs were analyzed immunohistochemically on tissue microarrays (TMAs). Additionally, the immunoreactivity of mitotic arrest defective protein 2 (MAD2) was also studied. The clinicopathological implications of these molecules were analyzed statistically.
  
  
  RESULTS
  High-level CDC20 protein expression (CDC20-H) was detected in 71 cases (19.6%). Additionally, CDC20-H was correlated with male sex, pT status, pleural invasion, and non-adenocarcinoma (non-ADC) histology. Significant correlation between CDC20 and MAD2 expression was found. NSCLC patients with tumor exhibiting CDC20-H showed significantly shorter 5-year overall survival (P=0.0007). According to subset analyses, CDC20-H was associated with shorter survival than CDC20-L only among ADC patients (P=0.0008), and not among squamous cell carcinoma (SCC) patients (P=0.5100). Importantly, CDC20-H was also identified as an independent prognostic factor in multivariate analysis (P=0.0065).
  
  
  CONCLUSIONS
  CDC20 was a negative prognostic marker with significance in patients with resected NSCLC, particularly those with ADC histology. These results provide additional information for determining postoperative adjuvant treatment.
authors.affiliation: Department of Thoracic Surgery, Sapporo Minami-Sanjo Hospital, Sapporo, Japan. katotatu7@msn.com
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Kato
authors.forename: Yataro
authors.initials: Y
authors.lastname: Daigo
authors.forename: Masato
authors.initials: M
authors.lastname: Aragaki
authors.forename: Keidai
authors.initials: K
authors.lastname: Ishikawa
authors.forename: Masaaki
authors.initials: M
authors.lastname: Sato
authors.forename: Mitsuhito
authors.initials: M
authors.lastname: Kaji
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry; D000328:Adult; D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000737:chemistry* / Q000401:mortality / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000737:chemistry; D064199:Cdc20 Proteins; D018797:Cell Cycle Proteins / Q000032:analysis* / Q000502:physiology; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000737:chemistry* / Q000401:mortality / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011379:Prognosis
chemical_list: D064199:Cdc20 Proteins; D018797:Cell Cycle Proteins; C577488:CDC20 protein, human