title: Formation of chitin-based nanomaterials using a chitin-binding peptide selected by phage-display.
abstract: Targeting polymers with peptides is an efficient strategy to functionalize biomaterials. Phage display technology is one of the most powerful techniques for selecting specific peptides for a wide variety of targets. A method to select a chitin-binding peptide from a 12-mer random peptide library was successfully performed against chitin immobilized in wells of microtiter plates. The synthetic chitin binding peptide (ChiBP) could bind to chitin beads and disrupt their structure. This selected peptide was successfully used to immobilize alkaline phosphatase on chitin. In addition, the peptide could induce colloidal chitin in water to form a chitin coat on the surface of plastic tubes. Scanning electron microscopy (SEM) revealed that the peptide could induce colloidal chitin and chitohexaose to form networks when the temperature was raised to 42°C.
authors.affiliation: Phage Display Biotechnology Laboratory, School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Thailand.
authors.forename: Feisal
authors.initials: F
authors.lastname: Khoushab
authors.forename: Nanthnit
authors.initials: N
authors.lastname: Jaruseranee
authors.forename: Waraporn
authors.initials: W
authors.lastname: Tanthanuch
authors.forename: Montarop
authors.initials: M
authors.lastname: Yamabhai
mesh_terms: D000469:Alkaline Phosphatase / Q000737:chemistry / Q000378:metabolism; D000595:Amino Acid Sequence; D002686:Chitin / Q000737:chemistry* / Q000378:metabolism; D056004:Molecular Dynamics Simulation; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D013499:Surface Properties
chemical_list: D019151:Peptide Library; D010455:Peptides; D002686:Chitin; D000469:Alkaline Phosphatase