title: Neuroprotective effects of adipose-derived stem cells against ischemic neuronal damage in the rabbit spinal cord.
abstract: Transplantation of adipose-derived stem cells (ASCs) is one of the possible therapeutic tools for ischemic damage. In this study, we observed the effects of ASCs against ischemic damage in the ventral horn of L(5-6) levels in the rabbit spinal cord. ASCs were isolated from rabbits, and cell type was confirmed by flow cytometry analysis, labeling with CM-DiI dye and differentiation into adipocytes in adipogenesis differentiation medium. ASCs were administered intrathecally into recipient rabbits (2 × 10⁵) immediately after reperfusion following a 15-min aortic artery occlusion in the subrenal region. Transplantation of ASCs significantly improved functions of the hindlimb and morphology of the ventral horn of spinal cord although CM-DiI-labeled ASCs were not observed in the spinal cord parenchyma. In addition, transplantation of ASCs significantly increased brain-derived neurotrophic factor (BDNF) levels at 72h after ischemia/reperfusion. These results suggest that transplantation of ASCs prevents motor neurons from spinal ischemic damage and reactive gliosis by increasing neurotrophic factors such as BDNF in the spinal cord.
authors.affiliation: Department of Neurology, Seoul National University Hospital, Seoul 110-744, South Korea.
authors.forename: Jin Young
authors.initials: JY
authors.lastname: Chung
authors.forename: Woosuk
authors.initials: W
authors.lastname: Kim
authors.forename: Wooseok
authors.initials: W
authors.lastname: Im
authors.forename: Dae Young
authors.initials: DY
authors.lastname: Yoo
authors.forename: Jung Hoon
authors.initials: JH
authors.lastname: Choi
authors.forename: In Koo
authors.initials: IK
authors.lastname: Hwang
authors.forename: Moo-Ho
authors.initials: MH
authors.lastname: Won
authors.forename: In Bok
authors.initials: IB
authors.lastname: Chang
authors.forename: Byung Moon
authors.initials: BM
authors.lastname: Cho
authors.forename: Hyung Sik
authors.initials: HS
authors.lastname: Hwang
authors.forename: Seung Myung
authors.initials: SM
authors.lastname: Moon
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000502:physiology; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D008297:Male; D009474:Neurons / Q000473:pathology*; D018696:Neuroprotective Agents / Q000008:administration & dosage*; D011817:Rabbits; D020760:Spinal Cord Ischemia / Q000473:pathology* / Q000601:surgery*; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000502:physiology*; D016896:Treatment Outcome
chemical_list: D018696:Neuroprotective Agents