title: Imaging protein interactions by FRET microscopy: cell preparation for FRET analysis.
abstract: The following protocol describes the preparation of cells for FRET analysis on live and fixed cells. The reagents used have been optimized to minimize the quenching of GFP mutants and fluorescent dyes.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Verveer
authors.forename: Oliver
authors.initials: O
authors.lastname: Rocks
authors.forename: Ailsa G
authors.initials: AG
authors.lastname: Harpur
authors.forename: Philippe I H
authors.initials: PI
authors.lastname: Bastiaens