title: A novel animal model for studying pancreatic regeneration by employing photochemical reaction.
abstract: OBJECTIVE
  We present photochemical-induced pancreatic necrosis (PIPN) as a novel induction method for studying pancreatic regeneration in an animal model.
  
  
  METHODS
  Photosensitive Rose Bengal was injected through the femoral vein in rats, followed by illumination of the surface of the pancreas with a cool halogen light for a period of 20 min. At 3, 6, and 24 h, and 7, 10, 14, and 20 days, experimental animals were sacrificed; all the animals received intravenous injection with 5-bromo-2-deoxyuridine (BrdU) 1 h prior to sacrifice.
  
  
  RESULTS
  At 3-6 h of induction of PIPN, pancreatic necrosis was superficially observed in the illuminated field. At 24 h, there was a slight increase in the depth and width of the lesion along with appearance of vascular congestion and thrombosis in the lesion. On days 7-10, the area of illumination was totally replaced by necrotic pancreatic tissue, inflammatory cell infiltrates, and newly appearing cellular components, including mesenchymal and epithelial cells, which formed tubular complexes. On day 14, clusters of tubular complexes intermingled with acinar cells, which were proven as newly formed acinar tissue by BrdU staining. On day 20, all the lesions had returned to a normal state of pancreatic tissue.
  
  
  CONCLUSIONS
  This study demonstrates the potential of PIPN as a valuable method for production of an animal model for studying healing processes or regeneration of pancreatic tissue after injury.
authors.affiliation: Department of Anatomy and Neurobiology, Kyung Hee University School of Medicine, Seoul, Republic of Korea.
authors.forename: Shengjun
authors.initials: S
authors.lastname: Han
authors.forename: Kwang Ro
authors.initials: KR
authors.lastname: Joo
authors.forename: Jung Won
authors.initials: JW
authors.lastname: Jeon
authors.forename: Jun Uk
authors.initials: JU
authors.lastname: Lim
authors.forename: Jae Jun
authors.initials: JJ
authors.lastname: Park
authors.forename: Hyun Phil
authors.initials: HP
authors.lastname: Shin
authors.forename: Jae Myung
authors.initials: JM
authors.lastname: Cha
authors.forename: Youngbuhm
authors.initials: Y
authors.lastname: Huh
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine; D008297:Male; D023421:Models, Animal; D009336:Necrosis; D010179:Pancreas / Q000473:pathology* / Q000502:physiology*; D010182:Pancreatic Diseases / Q000473:pathology; D010777:Photochemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration; D012395:Rose Bengal
chemical_list: D012395:Rose Bengal; D001973:Bromodeoxyuridine