title: A molecular approach to identification and profiling of first-line-drug-resistant mycobacteria from sputum of pulmonary tuberculosis patients.
abstract: Conventional and molecular techniques were applied to detect and characterize drug resistance of mycobacteria in the sputum samples of clinically confirmed tuberculosis. The sensitivities of mycobacterium detection by ZN staining, culture, multiplex PCR, and restriction fragment length polymorphism (RFLP) were 27.7%, 19.9%, 92.9%, and 95.7%, respectively, but all were 100% specific. The conventional and multiple-allele-specific PCR (MAS-PCR) methods enabled establishment of the drug resistance in 19.3% and 86.9% cases, respectively. We demonstrated that molecular techniques have potential in the accurate diagnosis of tuberculosis.
authors.affiliation: Department of Biochemistry, Institute of Postgraduate Medical Education and Research (IPGME&R), Kolkata, India.
authors.forename: Imran Hussain
authors.initials: IH
authors.lastname: Chowdhury
authors.forename: Aditi
authors.initials: A
authors.lastname: Sen
authors.forename: Bojlul
authors.initials: B
authors.lastname: Bahar
authors.forename: Avijit
authors.initials: A
authors.lastname: Hazra
authors.forename: Urmita
authors.initials: U
authors.lastname: Chakraborty
authors.forename: Subhadip
authors.initials: S
authors.lastname: Choudhuri
authors.forename: Avranil
authors.initials: A
authors.lastname: Goswami
authors.forename: Nishith Kumar
authors.initials: NK
authors.lastname: Pal
authors.forename: Basudev
authors.initials: B
authors.lastname: Bhattacharya
mesh_terms: D000995:Antitubercular Agents / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000302:isolation & purification*; D012150:Polymorphism, Restriction Fragment Length; D012680:Sensitivity and Specificity; D013183:Sputum / Q000382:microbiology*; D018088:Tuberculosis, Multidrug-Resistant / Q000175:diagnosis* / Q000382:microbiology; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis* / Q000382:microbiology
chemical_list: D000995:Antitubercular Agents