title: Garlicin attenuates reperfusion no-reflow in a catheter-based porcine model of acute myocardial infarction.
abstract: OBJECTIVE
  To evaluate whether garlicin can attenuate reperfusion no-reflow in a catheter-based porcine model of acute myocardial infarction (AMI).
  
  
  METHODS
  Twenty-two swine were used: six in a sham-operation group, and eight each in the control and garlicin groups. The distal part of the left anterior descending coronary artery (LAD) in the latter two groups was occluded by a dilated balloon for 2 hr, then reperfused for 3 hr. Garlicin (1.88mg/kg) was injected just before reperfusion until reperfusion for 1 hr in the garlicin group. Hemodynamic data were examined before AMI, 2 hr after occlusion, and 3 hr after reperfusion. Myocardial contrast echocardiography (MCE) and pathological staining were performed to evaluate the myocardial no-reflow area (NRA). Serum proinflammatory cytokines and endothelin (ET)-1 were examined by radioimmunoassay.
  
  
  RESULTS
  Left ventricular systolic pressure (LVSP) and left ventricular end-diastolic pressure (LVEDP) significantly improved in the garlicin group after reperfusion compared with the control group and also 2hr after AMI (p<0.05 for both). MCE and pathological staining both showed garlicin attenuated reperfusion NRA after AMI (p<0.05, p<0.01). Garlicin not only decreased serum interleukin (IL)-6 and tumor necrosis factor (TNF)-α after reperfusion (p<0.05 for both), but also ET-1 level (p<0.01).
  
  
  CONCLUSIONS
  Garlicin attenuated reperfusion no-reflow in our catheter-based porcrine model of AMI, possibly through decreasing serum proinflammatory cytokines and ET-1.
authors.affiliation: Department of Cardiology, China-Japan Friendship Hospital, Beijing, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Peng
authors.forename: Li
authors.initials: L
authors.lastname: Jiahui
authors.forename: Li
authors.initials: L
authors.lastname: Aili
authors.forename: Wang
authors.initials: W
authors.lastname: Yong
authors.forename: Shi
authors.initials: S
authors.lastname: Zaixiang
authors.forename: Ke
authors.initials: K
authors.lastname: Yuannan
authors.forename: Li
authors.initials: L
authors.lastname: Xianlun
mesh_terms: D000208:Acute Disease; D000498:Allyl Compounds / Q000494:pharmacology*; D000818:Animals; D057785:Catheters; D004195:Disease Models, Animal; D004220:Disulfides / Q000494:pharmacology*; D004452:Echocardiography; D005737:Garlic / Q000737:chemistry; D006439:Hemodynamics / Q000187:drug effects; D008297:Male; D009203:Myocardial Infarction / Q000150:complications*; D015428:Myocardial Reperfusion Injury / Q000150:complications* / Q000473:pathology / Q000503:physiopathology / Q000517:prevention & control*; D013440:Sulfides / Q000494:pharmacology
chemical_list: D000498:Allyl Compounds; D004220:Disulfides; D013440:Sulfides; C042577:diallyl trisulfide; C028009:diallyl disulfide