title: [Effect of electroacupuncture at "Fenglong" (ST 40) on rats with hyperlipidemia and its mechanism].
abstract: OBJECTIVE
  To explore the mechanism of electroacupuncture(EA) at "Fenglong" (ST 40) in hyperlipidemia (HLP) rats.
  
  
  METHODS
  Forty health SD rats were randomly divided into a normal control group (group A), a high fat forage feed group (group B) and a high fat forage feed treatment group (group C), a high fat forage + normal forage feed group (group D) and a high fat forage + normal forage feed treatment group (group E), eight rats in each group. EA was applied at "Fenglong" (ST 40) for the rats in group C and group E, once daily. After treatment of 30 days, blood lipid levels of rats, including total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C) in plasma were tested. Real time polymerase chain reaction (RT-PCR) and Western Blotting were applied to detect the gene expression changes of the contents of ATP-binding cassette transporter A1 (ABCA1), peroxisome proliferator-activated receptor alpha (PPARalpha), liver X receptor alpha (LXR-alpha) and retinoid X receptor alpha (RXR-alpha) in liver tissue of rats.
  
  
  RESULTS
  Compared with group A, the contents of TC, LDL-C were significantly elevated in group B and group D (all P < 0.01); compared with group B, above indices were significantly decreased in group D (all P < 0.01). After the treatment of EA at "Fenglong"(ST 40), the contents of TC, LDL-C were significantly decreased (all P < 0.01), and the contents of TG, HDL-C did not change obviously (all P > 0.05). Compared with group A, the mRNA and protein contents of ABCA1, PPARalpha, LXR-alpha and RXR-alpha were decreased obviously in group B and group D (all P < 0.01). But compared with group B, the above indices were decreased in the group D. There were signficantly increasing in the protein content of ABCA1, PPARalpha, RXR-alpha and LXR-alpha mRNA after the treatment of EA (all P < 0.05).
  
  
  CONCLUSIONS
  EA at "Fenglong" (ST 40) has some therapeutic effect on decrease the content of TC, LDL-C in rats of hyperlipemia and improve the gene expression of ABCA1, PPARalpha, LXR-alpha and RXR-alpha mRNA so as to promote reverse cholesterol transport.
authors.affiliation: Wuhan Hospital of Integrated Chinese and Western Medicine, The Affiliated Hospital of Hubei University of CM, Wuhan 430022, China. zhxzj99@yahoo.com.cn
authors.forename: Hong-Xing
authors.initials: HX
authors.lastname: Zhang
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wang
authors.forename: Hao
authors.initials: H
authors.lastname: Huang
authors.forename: Wei
authors.initials: W
authors.lastname: Yue
authors.forename: Peng-Fei
authors.initials: PF
authors.lastname: Qin
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D015669:Acupuncture Points; D000818:Animals; D002784:Cholesterol / Q000097:blood; D008076:Cholesterol, HDL / Q000097:blood; D015671:Electroacupuncture; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood / Q000235:genetics / Q000378:metabolism / Q000628:therapy*; D008297:Male; D047493:PPAR alpha / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014280:Triglycerides / Q000097:blood
chemical_list: D018528:ATP-Binding Cassette Transporters; D008076:Cholesterol, HDL; D047493:PPAR alpha; D014280:Triglycerides; D002784:Cholesterol