title: Atropa belladonna L. water extract: modulator of extracellular matrix formation in vitro and in vivo.
abstract: Previously, we found that treatment of cutaneous wounds with Atropa belladonna L. (AB) revealed shortened process of acute inflammation as well as increased tensile strength and collagen deposition in healing skin wounds (Gál et al. 2009). To better understand AB effect on skin wound healing male Sprague-Dawley rats were submitted to one round full thickness skin wound on the back. In two experimental groups two different concentrations of AB extract were daily applied whereas the control group remained untreated. For histological evaluation samples were removed on day 21 after surgery and stained for wide spectrum cytokeratin, collagen III, fibronectin, galectin-1, and vimentin. In addition, in the in vitro study different concentration of AB extract were used to evaluate differences in HaCaT keratinocytes proliferation and differentiation by detection of Ki67 and keratin-19 expressions. Furthermore, to assess ECM formation of human dermal fibroblasts on the in vitro level fibronectin and galectin-1 were visualized. Our study showed that AB induces fibronectin and galectin-1 rich ECM formation in vitro and in vivo. In addition, the proliferation of keratinocytes was also increased. In conclusion, AB is an effective modulator of skin wound healing. Nevertheless, further research is needed to find optimal therapeutic concentration and exact underlying mechanism of action.
authors.affiliation: East-Slovak Institute of Cardiovascular Diseases, Department for Biomedical Research, Košice, Slovak Republic. pgal@vusch.sk
authors.forename: P
authors.initials: P
authors.lastname: Gál
authors.forename: T
authors.initials: T
authors.lastname: Vasilenko
authors.forename: I
authors.initials: I
authors.lastname: Kováč
authors.forename: M
authors.initials: M
authors.lastname: Kostelníková
authors.forename: J
authors.initials: J
authors.lastname: Jakubčo
authors.forename: P
authors.initials: P
authors.lastname: Szabo
authors.forename: B
authors.initials: B
authors.lastname: Dvořánková
authors.forename: F
authors.initials: F
authors.lastname: Sabol
authors.forename: H-J
authors.initials: HJ
authors.lastname: Gabius
authors.forename: K
authors.initials: K
authors.lastname: Smetana
mesh_terms: D000818:Animals; D001532:Atropa belladonna / Q000737:chemistry; D002478:Cells, Cultured; D024061:Collagen Type III / Q000378:metabolism; D004195:Disease Models, Animal; D005109:Extracellular Matrix / Q000187:drug effects* / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D005353:Fibronectins / Q000378:metabolism; D037483:Galectin 1 / Q000378:metabolism; D006801:Humans; D053539:Keratin-19 / Q000378:metabolism; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000187:drug effects* / Q000293:injuries / Q000378:metabolism / Q000473:pathology; D012997:Solvents / Q000737:chemistry*; D013997:Time Factors; D014746:Vimentin / Q000378:metabolism; D014867:Water / Q000737:chemistry*; D014945:Wound Healing / Q000187:drug effects*; D014950:Wounds, Penetrating / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology
chemical_list: D024061:Collagen Type III; D005353:Fibronectins; D037483:Galectin 1; D053539:Keratin-19; D010936:Plant Extracts; D012997:Solvents; D014746:Vimentin; D014867:Water