title: Interlaboratory comparison of an analytical method for the determination of semduramicin in poultry feed at the authorized level using high-performance liquid chromatography coupled to postcolumn derivatization and spectrophotometric detection.
abstract: The performance characteristics of a method based on HPLC with postcolumn derivatization and spectrophotometric detection for the quantification of semduramicin in poultry feedingstuffs have been determined via a collaborative study. Semduramicin is a feed additive that is authorized for fattening chickens within the European Union at a minimum and maximum content of 20 and 25 mg/kg in feedingstuffs, respectively. The target concentration of semduramicin in the test samples ranged from 11.5 to 45.0 mg/kg. The study has been conducted with two different types of test material, namely, feedingstuff samples that have been previously ground in our laboratory and pelleted feedingstuffs. In the latter case, the laboratories participating in the study had to grind the samples prior to analysis. The obtained RSD for repeatability (RSD(r)) ranged from 2 to 10% for the ground materials, and from 2 and 7% for the pelleted materials. The RSD for reproducibility (RSDR) varied between 11 and 16% for the ground materials, and between 12 and 15% for the pelleted materials. These data indicated that grinding as an additional step in the analytical procedure did not influence the precision profile of the method. In addition, the HorRat values for all test materials were below or equal to 1.5, thus demonstrating that the obtained precision data were acceptable for the purpose of the method. Furthermore, an estimation of trueness based on statistical treatment of the results reported from the laboratories for spiked samples revealed acceptable mean recovery values of 88 +/- 4%. Based on the obtained performance profile, the method can be considered fully validated and transferable to control laboratories to be used within the framework of official control.
authors.affiliation: European Commission, Joint Research Centre, Institute for Reference Materials and Measurements (EC-JRC-IRMM), Retieseweg 111, B-2440 Geel, Belgium. maria-jose.gonzalez-de-la-huebra@ec.europa.eu
authors.forename: María José
authors.initials: MJ
authors.lastname: González de la Huebra
authors.forename: Ursula
authors.initials: U
authors.lastname: Vincent
authors.forename: Federica
authors.initials: F
authors.lastname: Serano
authors.forename: Christoph
authors.initials: C
authors.lastname: von Holst
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D057230:Limit of Detection; D009550:Nigericin / Q000031:analogs & derivatives* / Q000032:analysis; D011200:Poultry; D012015:Reference Standards; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
chemical_list: D000900:Anti-Bacterial Agents; C072941:semduramicin; D009550:Nigericin