title: Genetic analysis of liver metastatic cell lines with different metastatic potential.
abstract: Metastasis is the major feature of malignant tumors that causes 90% of cancer deaths. Our laboratory has already established liver metastatic clones with YCC-16, isolated from the blood of a gastric cancer patient and expanded in vitro culture using a repeated orthotopic implantation method, and had reported biologic behaviour of the parental YCC-16, the orthotopic primary S1L0, and S1L1, S2L2 and S3L3 liver metastatic clones. Here, using these cell lines, we screened from chromosomal abnormalities using karyotype analysis and micro-CGH matching. There were 31 genes screened using PCA method which were functionally related to cell adhesion. Also, there were 23 genes selected which were related to the liver specific metastasis but excluded genes related to adhesion. There were 4 genes which demonstrated reduced or increased expression stepwise with passage. In conclusion, our results should contribute to exploring the mechanisms of liver metastasis by gastric cancer.
authors.affiliation: Department of Oncology, Yanbian University Affiliated Hospital, Yanji China.
authors.forename: Hong-Hua
authors.initials: HH
authors.lastname: Sun
authors.forename: Hai
authors.initials: H
authors.lastname: Cui
authors.forename: Yong-Min
authors.initials: YM
authors.lastname: Jin
authors.forename: Xiong
authors.initials: X
authors.lastname: Cui
authors.forename: Long-Zhen
authors.initials: LZ
authors.lastname: Piao
authors.forename: Song-Nan
authors.initials: SN
authors.lastname: Zhang
authors.forename: Hyun-Cheol
authors.initials: HC
authors.lastname: Chung
authors.forename: Xiong-Hu
authors.initials: XH
authors.lastname: Shen
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000235:genetics*; D002448:Cell Adhesion; D002869:Chromosome Aberrations; D005260:Female; D020869:Gene Expression Profiling; D005820:Genetic Testing; D006801:Humans; D007621:Karyotyping; D008113:Liver Neoplasms / Q000235:genetics* / Q000556:secondary*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D020411:Oligonucleotide Array Sequence Analysis; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: D014408:Biomarkers, Tumor