title: Three-dimensional super-localization and tracking of single gold nanoparticles in cells.
abstract: We introduce a precise three-dimensional (3D) localization method of spherical gold nanoparticle probes using model-based correlation coefficient mapping. To accomplish this, a stack of sample images at different z-positions are acquired, and a 3D intensity profile of the probe serving as the model is used to map out the positions of nanoparticles in the sample. By using this model-based correlation imaging method, precise localization can be achieved in imaging techniques with complicated point spread functions (PSF) such as differential interference contrast (DIC) microscopy. We demonstrated the localization precision of 4-7 nm laterally and 16 nm axially for 40-nm gold nanospheres at an imaging rate of 10 frames per second. The 3D superlocalization method was applied to tracking gold nanospheres during live endocytosis events.
authors.affiliation: Ames Laboratory, U.S. Department of Energy, and Department of Chemistry, Iowa State University, Ames, Iowa 50011, USA.
authors.forename: Yan
authors.initials: Y
authors.lastname: Gu
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Di
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.forename: Gufeng
authors.initials: G
authors.lastname: Wang
authors.forename: Ning
authors.initials: N
authors.lastname: Fang
mesh_terms: D000465:Algorithms; D002470:Cell Survival; D004705:Endocytosis; D006046:Gold / Q000032:analysis*; D006367:HeLa Cells; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008857:Microscopy, Interference / Q000379:methods*; D053758:Nanoparticles / Q000032:analysis*
chemical_list: D006046:Gold