title: Down regulation of CD24 and HER-2/neu in breast carcinoma cells by activated human dendritic cell. Role of STAT3.
abstract: Human dendritic cells (DCs) stimulated with cytokines and LPS down regulate the expression of proto-oncogene HER-2/neu and GPI linked protein CD24 in breast cancer cell lines. We demonstrated that naïve DC from human peripheral blood, when stimulated with IFN-γ, IL-15 or LPS reduces the expression of HER-2/neu and CD24, via activation of TNF-α. Pretreatment of tumor cells with STAT3 specific inhibitors or knocking down of STAT3 by SiRNA makes the tumor cell more susceptible to apoptosis and DC mediated inhibition of both CD24 and HER-2/neu. Thus DC could acts as an inhibitory regulator in suppressing oncogene and prevention of metastasis.
authors.affiliation: Immunobiology Laboratory, Department of Zoology, Banaras Hindu University, Varanasi, India.
authors.forename: Sumit Kumar
authors.initials: SK
authors.lastname: Hira
authors.forename: Partha Pratim
authors.initials: PP
authors.lastname: Manna
mesh_terms: D001943:Breast Neoplasms / Q000276:immunology*; D051927:CD24 Antigen / Q000276:immunology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D000090063:Proto-Oncogene Mas; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics / Q000276:immunology*; D050796:STAT3 Transcription Factor / Q000276:immunology*
chemical_list: D051927:CD24 Antigen; D000090063:Proto-Oncogene Mas; D018719:Erb-b2 Receptor Tyrosine Kinases; D050796:STAT3 Transcription Factor; C497889:CD24 protein, human; C000715470:MAS1 protein, human; C494086:STAT3 protein, human; C508053:ERBB2 protein, human