title: Neuroprotective effects of phlorotannins isolated from a brown alga, Ecklonia cava, against H2O2-induced oxidative stress in murine hippocampal HT22 cells.
abstract: Exposure of neurons to hydrogen peroxide (H(2)O(2)) results in oxidative stress and the activation of a cascade of intracellular toxic events resulting in oxidation, lipid peroxidation, and Ca(2+) elevation, ultimately resulting in cell death. In this study, we attempted to characterize the neuroprotective effects of phlorotannins isolated from Ecklonia cava, including phloroglucinol, eckol, triphloroethol A, eckstolonol, and dieckol, against H(2)O(2)-induced cell damage in murine hippocampus neuronal (HT22) cells. We measured the reactive oxygen species (ROS) and lipid peroxidation levels and evaluated the resultant cell death and alterations in Ca(2+)-concentrations. All phlorotannins were to scavenge intracellular ROS and repress ROS accumulation, thus preventing lipid peroxidation. Consquently, all phlorotannins reduced H(2)O(2)-induced cell death in HT22 cells. Moreover, phlorotannins inhibited H(2)O(2)-induced Ca(2+) release. This study provides a new useful strategy for preventing neuronal H(2)O(2)-induced oxidative stress.
authors.affiliation: Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Sung-Myung
authors.initials: SM
authors.lastname: Kang
authors.forename: Seon-Heui
authors.initials: SH
authors.lastname: Cha
authors.forename: Ju-Young
authors.initials: JY
authors.lastname: Ko
authors.forename: Min-Cheol
authors.initials: MC
authors.lastname: Kang
authors.forename: Daekyung
authors.initials: D
authors.lastname: Kim
authors.forename: Soo-Jin
authors.initials: SJ
authors.lastname: Heo
authors.forename: Jin-Soo
authors.initials: JS
authors.lastname: Kim
authors.forename: Min Soo
authors.initials: MS
authors.lastname: Heu
authors.forename: Yong-Tae
authors.initials: YT
authors.lastname: Kim
authors.forename: Won-Kyo
authors.initials: WK
authors.lastname: Jung
authors.forename: You-Jin
authors.initials: YJ
authors.lastname: Jeon
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006571:Heterocyclic Compounds / Q000494:pharmacology*; D006624:Hippocampus / Q000166:cytology; D006861:Hydrogen Peroxide / Q000633:toxicity; D015227:Lipid Peroxidation / Q000187:drug effects; D051379:Mice; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D000459:Phaeophyceae; D010696:Phloroglucinol / Q000031:analogs & derivatives* / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D006571:Heterocyclic Compounds; D018696:Neuroprotective Agents; D017382:Reactive Oxygen Species; C504400:triphlorethol-A; D006861:Hydrogen Peroxide; D010696:Phloroglucinol; D002118:Calcium