title: Adaptation of a trap door technique for the recovery of ovarian cortical biopsies from Cebus apella (capuchin monkey).
abstract: There is a paucity of efficient cryopreservation protocols for primordial follicles enclosed in the ovarian tissue from non-human primates (NHP), in special New World primates. Our objective was to establish an optimal procedure for the recovery of ovarian biopsies from capuchin monkeys. To this end, we adapted a trap door biopsy method. Follicular density and quality of the biopsies were evaluated and ultrasound analysis was performed before and continuously after surgery to assess ovarian structure. Ovarian tissue biopsies recovered by the trap door technique allowed the successful harvesting of primordial follicles from capuchin monkeys, and no complication was recorded. The female cycle was not affected by surgery and no adherence was found thereafter. In conclusion, the adaptation of a trap door biopsy method is a safe procedure and allows recovery of healthy primordial follicles.
authors.affiliation: Laboratory of Wild Animal Biology and Medicine, Universidade Federal do Pará, Brazil.
authors.forename: Luana N
authors.initials: LN
authors.lastname: Santana
authors.forename: Adriel B
authors.initials: AB
authors.lastname: Brito
authors.forename: Danielle C
authors.initials: DC
authors.lastname: Brito
authors.forename: Julianne S
authors.initials: JS
authors.lastname: Lima
authors.forename: Sheyla F S
authors.initials: SF
authors.lastname: Domingues
authors.forename: Regiane R
authors.initials: RR
authors.lastname: Santos
mesh_terms: D000818:Animals; D001706:Biopsy; D002428:Cebus; D005260:Female; D006801:Humans; D008853:Microscopy; D006080:Ovarian Follicle / Q000166:cytology / Q000000981:diagnostic imaging / Q000601:surgery*; D019651:Plastic Surgery Procedures / Q000379:methods*; D018616:Ultrasonography, Doppler, Duplex