title: [Effects of TFDP3 on regulating the autophagy and apoptosis of LNCaP cells].
abstract: OBJECTIVE
  To investigate the effect of TFDP3 on prostate cancer cell line LNCaP by transgenic method, and to explore the effect of TFDP3 on regulating the autophay and apoptosis by co-regulation with E2F1.
  
  
  METHODS
  LNCaP cells were transfected with pcDNA3.1-TFDP3, pCMV-E2F1-HA or pcDNA3.1 empty vector.The expression of TFDP3, E2F1 and LC3B were detected by real-time PCR after transfection for 24 h. Western blotting was used to monitor the changes in autophagy-associated protein LC3B, Apoptosis of transfected cells were analyzed by flow cytometry.
  
  
  RESULTS
  The results showed that activation of TFDP3 upregulates the expression of autophagy genes-microtubule-associated protein-1 light chain-3B (LC3B), and E2F1 antagonizes TFDP3-induced autophagy, and TFDP3 can inhibit E2F1-induced apoptosis.
  
  
  CONCLUSIONS
  TFDP3 upregulates the expression of autophagy gene LC3B and inhibits E2F1-induced apoptosis, and may play an important role in prostate cancer.
authors.affiliation: Center for Clinical Laboratory Medicine of People's Liberation Army, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. renlifen2004@163.com
authors.forename: Li-Fen
authors.initials: LF
authors.lastname: Ren
authors.forename: Yue-Yun
authors.initials: YY
authors.lastname: Ma
authors.forename: Qiao-Hong
authors.initials: QH
authors.lastname: Yue
authors.forename: Ming-Quan
authors.initials: MQ
authors.lastname: Su
authors.forename: Xiao-Ke
authors.initials: XK
authors.lastname: Hao
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D001343:Autophagy / Q000235:genetics*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D050687:E2F1 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008869:Microtubule-Associated Proteins / Q000235:genetics* / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050680:Transcription Factor DP1 / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
chemical_list: D050687:E2F1 Transcription Factor; C493753:E2F1 protein, human; C502070:MAP1LC3B protein, human; D008869:Microtubule-Associated Proteins; C516920:TFDP3 protein, human; D050680:Transcription Factor DP1