title: Identification and biocontrol efficacy of Streptomyces miharaensis producing filipin III against Fusarium wilt.
abstract: A number of bacterial strains were isolated from the internal tissue of Trapa japonica. Of these, strain KPE62302H, which had a 16S rDNA sequence identical to that of Streptomyces miharaensis showed antifungal activity against several plant pathogens. Treatment of seeds with strain KPE62302H induced a significant reduction in the incidence of Fusarium wilt in tomato plants compared with untreated controls. An antifungal substance (FP-1) was purified from the culture extract of strain KPE62302H using C18 flash and Sephadex LH-20 column chromatography and reverse phase HPLC. Extensive spectrometric analysis using MS and NMR identified this as filipin III. FP-1 inhibited the mycelial growth of plant pathogenic fungi such as Alternaria mali, Aspergillus niger, Colletotrichum gloeosporioides, C. orbiculare, Cylindrocarpon destructans, Diaporthe citiri, Fusarium oxysporum at 1-10 μg ml(-1) and also markedly inhibited the development of Fusarium wilt caused by F. oxysporum f.sp. lycopersici in tomato plants by treatment with 10 μg ml(-1) under greenhouse conditions. The efficacy of FP-1 against Fusarium wilt was comparable to that of the synthetic fungicide benomyl. An egfp -tagged strain of KPE62302H confirmed its ability to colonize tomato plants.
authors.affiliation: Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea.
authors.forename: Jeong Do
authors.initials: JD
authors.lastname: Kim
authors.forename: Jae Woo
authors.initials: JW
authors.lastname: Han
authors.forename: In Cheon
authors.initials: IC
authors.lastname: Hwang
authors.forename: Dongho
authors.initials: D
authors.lastname: Lee
authors.forename: Beom Seok
authors.initials: BS
authors.lastname: Kim
mesh_terms: D061046:Biological Control Agents; D005372:Filipin / Q000494:pharmacology*; D005659:Fungicides, Industrial / Q000494:pharmacology; D005670:Fusarium / Q000187:drug effects / Q000472:pathogenicity*; D018551:Solanum lycopersicum / Q000382:microbiology; D010935:Plant Diseases / Q000382:microbiology / Q000517:prevention & control*; D011720:Pyrazoles / Q000494:pharmacology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012639:Seeds / Q000382:microbiology; D013302:Streptomyces / Q000235:genetics / Q000502:physiology*
chemical_list: D061046:Biological Control Agents; D005659:Fungicides, Industrial; D011720:Pyrazoles; D012336:RNA, Ribosomal, 16S; C050097:compound FP 1; D005372:Filipin