title: Effects of immunization with group a streptococcal antigens on the transplantability of mouse bone marrow stromal stem cells, counts of stromal precursor cells, and their osteogenic characteristics.
abstract: Immunization of CBA mice with killed group A streptococcus (type 5) vaccine changed the counts of stromal precursor cells (CFC-F) in bone marrow transplants at different donor-recipient combinations (normal, N, or immune, I). CFC-F counts in bone marrow transplants from normal mice transplanted to immunized animals decreased 4-6-fold depending on the transplant age in comparison with similar transplants in normal recipients. The percentage of CFC-F colonies with alkaline phosphatase (osteogenesis marker) activity decreased more than 2-fold. Similarly, the count of CFC-F in the transplants was 2-fold lower during delayed (7 months) period after bone marrow transplantation from immunized donors (8-12 days after the end of immunization) to intact recipients, while 2 months after transplantation it was 3-fold lower. The mean optical density of the bone capsule in preparations stained for glycogen and alkaline phosphatase was 1.5-3 times lower in the N-->I and I-->N experiments in comparison with the control (N-->N). On the other hand, CFC-F count in the femoral bone marrow of immunized animals was significantly (3.5-2.5 times) higher during the period from 8 days to 8 months after the end of immunization compared to CFC-F count in the femoral bone marrow of intact mice. These results attest to a significant prolonged effect of streptococcal antigens on the bone marrow stromal tissue. These data also indicate that not all CFC-F, the counts of which increased in response to antigens, are responsible for transplantability of the stromal tissue in heterotopic transplantation. Immunization by streptococcal antigens seemed to suppress transplantability and osteogenic activity of stromal stem cells. The efficiency of CFC-F cloning in mouse bone marrow cultures increased significantly (2-3-fold) in the presence of sera from immune mice. The levels of TNF-α and IFN-γ were low in this serum (2.7 and 6 times lower, respectively) in comparison with normal serum. Presumably, the effects of streptococcal antigens on stromal tissue were mediated through serum cytokines.
authors.affiliation: Laboratory of Immunity Regulation, N. F. Gamaleya Institute of Epidemiology and Microbiology, Russian Academy of Medical Sciences, Moscow, Russia. Uliya.Gorskaya@nearmedic.ru
authors.forename: Yu F
authors.initials: YF
authors.lastname: Gorskaya
authors.forename: T A
authors.initials: TA
authors.lastname: Danilova
authors.forename: O V
authors.initials: OV
authors.lastname: Lebedinskaya
authors.forename: V G
authors.initials: VG
authors.lastname: Lunin
authors.forename: V I
authors.initials: VI
authors.lastname: Grabko
authors.forename: N E
authors.initials: NE
authors.lastname: Sharapova
authors.forename: V G
authors.initials: VG
authors.lastname: Nesterenko
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000008:administration & dosage / Q000276:immunology*; D001854:Bone Marrow Cells / Q000166:cytology; D016026:Bone Marrow Transplantation / Q000276:immunology*; D002452:Cell Count; D002478:Cells, Cultured; D016207:Cytokines / Q000097:blood; D005269:Femur / Q000166:cytology; D006168:Guinea Pigs; D051379:Mice; D008808:Mice, Inbred CBA; D010012:Osteogenesis / Q000276:immunology; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology; D022541:Streptococcal Vaccines / Q000008:administration & dosage / Q000276:immunology; D013297:Streptococcus pyogenes / Q000276:immunology*; D017154:Stromal Cells / Q000166:cytology / Q000637:transplantation; D016042:Transplantation, Heterotopic; D014611:Vaccination
chemical_list: D000942:Antigens, Bacterial; D016207:Cytokines; D022541:Streptococcal Vaccines