title: Determination of glucose and uric acid with bienzyme colorimetry on microfluidic paper-based analysis devices.
abstract: In this work, we first employ a drying method combining with the bienzyme colorimetric detection of glucose and uric acid on microfluidic paper-based analysis devices (μPADs). The channels of 3D μPADs are also designed by us to get better results. The color results are recorded by both Gel Documentation systems and a common camera. By using Gel Documentation systems, the limits of detection (LOD) of glucose and uric acid are 3.81 × 10(-5)M and 4.31 × 10(-5)M, respectively one order of magnitude lower than that of the reported methods on μPADs. By using a common camera, the limits of detection (LOD) of glucose and uric acid are 2.13 × 10(-4)M and 2.87 × 10(-4)M, respectively. Furthermore, the effects of detection conditions have been investigated and discussed comprehensively. Human serum samples are detected with satisfactory results, which are comparable with the clinical testing results. A low-cost, simple and rapid colorimetric method for the simultaneous detection of glucose and uric acid on the μPADs has been developed with enhanced sensitivity.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan 430071, China.
authors.forename: Fubin
authors.initials: F
authors.lastname: Wang
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Xiang
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Luo
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
authors.forename: Xinghu
authors.initials: X
authors.lastname: Ji
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China. Electronic address: zhkhe@whu.edu.cn.
authors.forename: Zhike
authors.initials: Z
authors.lastname: He
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods / Q000706:statistics & numerical data; D001786:Blood Glucose / Q000032:analysis; D003124:Colorimetry / Q000379:methods; D004867:Equipment Design; D005947:Glucose / Q000032:analysis*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods / Q000706:statistics & numerical data; D010209:Paper; D014527:Uric Acid / Q000032:analysis* / Q000097:blood
chemical_list: D001786:Blood Glucose; D014527:Uric Acid; D005947:Glucose