title: Mitogenic activity of sulfated chitosan and cellulose derivatives is related to protection of FGF-2 from proteolytic cleavage.
abstract: Novel chitosan (CHS) and cellulose sulfates (CSs) are studied regarding their mitogenic activity and their protective effect against proteolytic digestion of FGF-2. An intermediate degree of sulfation (DS(S) ) and lower concentration of CHS have superior effect on 3T3 cell growth while the mitogenic activity of CS increases with DS(S)  and concentration. Experiments with trypsin as model proteinase show that protection of FGF-2 from proteolytic digestion depends on DS(S)  and the concentration of derivatives in the same manner as cell growth. Studies on stability of FGF-2 added to cultures of 3T3 cells show that the FGF-2 concentration remains higher in the presence of derivatives. Results indicate that the mitogenic activity of CHS and CS is due to protection of FGF-2 from proteolytic cleavage.
authors.affiliation: Department of Pharmaceutics and Biopharmaceutics, Institute of Pharmacy, Martin Luther University Halle-Wittenberg, Heinrich-Damerow-Strasse 4, 06120 Halle-Saale, Germany.
authors.forename: Annett
authors.initials: A
authors.lastname: Weltrowski
authors.forename: Marie-Luise
authors.initials: ML
authors.lastname: da Silva Almeida
authors.forename: Dieter
authors.initials: D
authors.lastname: Peschel
authors.forename: Kai
authors.initials: K
authors.lastname: Zhang
authors.forename: Steffen
authors.initials: S
authors.lastname: Fischer
authors.forename: Thomas
authors.initials: T
authors.lastname: Groth
mesh_terms: D041721:3T3-L1 Cells; D000818:Animals; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry* / Q000378:metabolism; D051379:Mice; D008934:Mitogens / Q000737:chemistry* / Q000494:pharmacology; D059748:Proteolysis; D014357:Trypsin / Q000737:chemistry
chemical_list: D008934:Mitogens; C480355:chitosan sulfate; D016222:Fibroblast Growth Factor 2; D002482:Cellulose; D048271:Chitosan; C020254:cellulose sulfate; D014357:Trypsin