title: Rapid detection of duck hepatitis virus type-1 by reverse transcription loop-mediated isothermal amplification.
abstract: A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of duck hepatitis virus type-1 (DHV-1) was established. Using primers specific to the highly conserved 3D gene of DHV-1, the developed RT-LAMP assay detected the viral RNA of DHV-1 extracted from both allantoic fluid and liver samples of infected ducks. The assay is as sensitive as RT-PCR, and shows no cross-reaction with other common avian viral and bacterial pathogens. In addition to detection via ethidium bromide staining following gel electrophoresis, naked-eye observation after staining with SYBR Green I dye can be used to detect RT-LAMP products; this enables field application of this assay. The findings demonstrate that RT-LAMP can serve as a helpful tool for the detection and surveillance of DHV-1 in the poultry industry.
authors.affiliation: Shanghai Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Shanghai 200241, China.
authors.forename: Cuiping
authors.initials: C
authors.lastname: Song
authors.forename: Hongquan
authors.initials: H
authors.lastname: Wan
authors.forename: Shengqing
authors.initials: S
authors.lastname: Yu
authors.forename: Xiangan
authors.initials: X
authors.lastname: Han
authors.forename: Xusheng
authors.initials: X
authors.lastname: Qiu
authors.forename: Qinghai
authors.initials: Q
authors.lastname: Hu
authors.forename: Lei
authors.initials: L
authors.lastname: Tan
authors.forename: Chan
authors.initials: C
authors.lastname: Ding
mesh_terms: D000482:Allantois / Q000821:virology; D000818:Animals; D052160:Benzothiazoles; D003429:Cross Reactions; D017931:DNA Primers / Q000235:genetics; D003959:Diamines; D004372:Ducks; D004587:Electrophoresis, Agar Gel; D004371:Hepatitis Virus, Duck / Q000235:genetics / Q000302:isolation & purification*; D006524:Hepatitis, Viral, Animal / Q000821:virology*; D008099:Liver / Q000821:virology; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D009930:Organic Chemicals; D010850:Picornaviridae Infections / Q000662:veterinary* / Q000821:virology; D011804:Quinolines; D048348:Reverse Transcription; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods; D014773:Virology / Q000379:methods*
chemical_list: D052160:Benzothiazoles; D017931:DNA Primers; D003959:Diamines; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I