title: Efficient and specific analysis of red blood cell glycerophospholipid fatty acid composition.
abstract: BACKGROUND
  Red blood cell (RBC) n-3 fatty acid status is related to various health outcomes. Accepted biological markers for the fatty acid status determination are RBC phospholipids, phosphatidylcholine, and phosphatidyletholamine. The analysis of these lipid fractions is demanding and time consuming and total phospholipid n-3 fatty acid levels might be affected by changes of sphingomyelin contents in the RBC membrane during n-3 supplementation.
  
  
  OBJECTIVE
  We developed a method for the specific analysis of RBC glycerophospholipids. The application of the new method in a DHA supplementation trial and the comparison to established markers will determine the relevance of RBC GPL as a valid fatty acid status marker in humans.
  
  
  METHODS
  Methyl esters of glycerophospholipid fatty acids are selectively generated by a two step procedure involving methanolic protein precipitation and base-catalysed methyl ester synthesis. RBC GPL solubilisation is facilitated by ultrasound treatment. Fatty acid status in RBC glycerophospholipids and other established markers were evaluated in thirteen subjects participating in a 30 days supplementation trial (510 mg DHA/d).
  
  
  RESULTS
  The intra-assay CV for GPL fatty acids ranged from 1.0 to 10.5% and the inter-assay CV from 1.3 to 10.9%. Docosahexaenoic acid supplementation significantly increased the docosahexaenoic acid contents in all analysed lipid fractions. High correlations were observed for most of the mono- and polyunsaturated fatty acids, and for the omega-3 index (r = 0.924) between RBC phospholipids and glycerophospholipids. The analysis of RBC glycerophospholipid fatty acids yields faster, easier and less costly results equivalent to the conventional analysis of RBC total phospholipids.
authors.affiliation: Division of Metabolic and Nutritional Medicine, Dr von Hauner Children's Hospital, University of Munich Medical Center, Munich, Germany.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Klem
authors.forename: Mario
authors.initials: M
authors.lastname: Klingler
authors.forename: Hans
authors.initials: H
authors.lastname: Demmelmair
authors.forename: Berthold
authors.initials: B
authors.lastname: Koletzko
mesh_terms: D000328:Adult; D004912:Erythrocytes / Q000737:chemistry*; D005227:Fatty Acids / Q000032:analysis*; D015525:Fatty Acids, Omega-3 / Q000032:analysis; D005260:Female; D020404:Glycerophospholipids / Q000737:chemistry*; D006801:Humans; D008297:Male; D015203:Reproducibility of Results
chemical_list: D005227:Fatty Acids; D015525:Fatty Acids, Omega-3; D020404:Glycerophospholipids