title: Fluorometric determination of inulin using 5-quinolineboronic acid and inulinase.
abstract: Inulin is a polysaccharide composed mainly of D-fructose units and is the most reliable indicator of the glomerular filtration rate. We have proposed an inulin detection method that involves the hydrolysis of inulin to D-fructose using inulinase and the selective binding of D-fructose from inulin using 5-quinolineboronic acid. In this method, the fluorescence of 5-quinolineboronic acid increases, depending on inulin concentration. For inulin in plasma, the detection and quantitation limits were calculated to be 3.7 and 11 μg/ml, respectively.
authors.affiliation: Faculty of Pharmaceutical Sciences, Josai University, Sakado, Saitama 350-0295, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Rikita
authors.forename: Yuya
authors.initials: Y
authors.lastname: Egawa
authors.forename: Toshinobu
authors.initials: T
authors.lastname: Seki
mesh_terms: D001897:Boronic Acids / Q000737:chemistry*; D005470:Fluorometry; D005632:Fructose / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007444:Inulin / Q000032:analysis*; D011804:Quinolines / Q000737:chemistry*
chemical_list: C575032:5-quinolineboronic acid; D001897:Boronic Acids; D011804:Quinolines; D005632:Fructose; D007444:Inulin; D006026:Glycoside Hydrolases; C023086:inulinase