title: Suppression of astrocyte lineage in adult hippocampal progenitor cells expressing hippocampal cholinergic neurostimulating Peptide precursor in an in vivo ischemic model.
abstract: Hippocampal cholinergic neurostimulating peptide (HCNP) is known to promote differentiation of septohippocampal cholinergic neurons. The HCNP precursor protein (HCNP-pp) may play several roles, for example, as an ATP-binding protein, a Raf kinase inhibitor protein, and a phosphatidylethanolamine-binding  protein, as well as a precursor for HCNP. This study therefore aimed to elucidate the involvement of HCNP-pp in specific neural lineages after stroke using a hypoxic-ischemic (HI) rat model of brain ischemia. The specific neural lineages in the hippocampus were investigated 14 days after ischemia.  Some bromodeoxyuridine (BrdU)(+) neural progenitor cells in the hippocampus of hypoxic, HI, or sham-operated rats expressed HCNP-pp. Almost half of the BrdU(+)/HCNP-pp(+) cells also expressed the oligodendrocyte lineage marker 2',3'-cyclic nucleotide  3'-phosphodiesterase, whereas only a few BrdU(+)/HCNP-pp(+) cells in the hippocampus in HI brains expressed the neuronal lineage marker, doublecortin (DCX). Interestingly, no BrdU(+)/HCNP-pp(+) progenitor cells in hypoxic, HI, or sham-operated brains  expressed the astrocyte lineage marker, glial fibrillary acidic protein. Together with previous in vitro data, the results of this study suggest that the expression level of HCNP-pp regulates the differentiation of neural progenitor cells into specific neural lineages in the HI hippocampus,  indicating that neural stem cell fate can be controlled via the HCNP-pp mediating pathway.
authors.affiliation: Department of Neurology, Nagoya City University Graduate School of Medical Sciences, Nagoya, Aichi, Japan.
authors.forename: Takanari
authors.initials: T
authors.lastname: Toyoda
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Matsukawa
authors.forename: Takafumi
authors.initials: T
authors.lastname: Sagisaka
authors.forename: Norihiko
authors.initials: N
authors.lastname: Uematsu
authors.forename: Tetsuko
authors.initials: T
authors.lastname: Kanamori
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kato
authors.forename: Masayuki
authors.initials: M
authors.lastname: Mizuno
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Wake
authors.forename: Hideki
authors.initials: H
authors.lastname: Hida
authors.forename: Cesario V
authors.initials: CV
authors.lastname: Borlongan
authors.forename: Kosei
authors.initials: K
authors.lastname: Ojika
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology* / Q000378:metabolism; D002545:Brain Ischemia / Q000378:metabolism / Q000473:pathology*; D002454:Cell Differentiation / Q000502:physiology; D048708:Cell Growth Processes / Q000502:physiology; D019070:Cell Lineage; D004195:Disease Models, Animal; D000089662:Doublecortin Protein; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism; D049488:Phosphatidylethanolamine Binding Protein / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: C000714848:Dcx protein, rat; D000089662:Doublecortin Protein; D049488:Phosphatidylethanolamine Binding Protein