title: Application of creatinine-sensitive biosensor for hemodialysis control.
abstract: The highly sensitive and selective potentiometric biosensor for creatinine determination has been developed by us earlier. In it, pH-sensitive field effect transistors were used as transducer and immobilized creatinine deiminase (EC 3.5.4.21)--as a biosensitive element. In the work presented, we optimized this biosensor for creatinine analysis in real samples of dialysate in patients with renal failure. The optimized version of biosensor was applied for on-line monitoring of the level of creatinine in the patient's dialysate fluid in the course of dialysis session. High correlation between the biosensor analysis and traditional Jaffe method was demonstrated.
authors.affiliation: Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Zabolotnogo St., 03143 Kyiv, Ukraine. Electronic address: helen_nazarenko@yahoo.com.
authors.forename: O A
authors.initials: OA
authors.lastname: Zinchenko
authors.affiliation: Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Zabolotnogo St., 03143 Kyiv, Ukraine.
authors.forename: S V
authors.initials: SV
authors.lastname: Marchenko
authors.affiliation: Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Zabolotnogo St., 03143 Kyiv, Ukraine.
authors.forename: T A
authors.initials: TA
authors.lastname: Sergeyeva
authors.affiliation: Institute of Semiconductor Physics, National Academy of Sciences of Ukraine, 41 Nauki Prosp., 03028 Kyiv, Ukraine.
authors.forename: A L
authors.initials: AL
authors.lastname: Kukla
authors.affiliation: Institute of Semiconductor Physics, National Academy of Sciences of Ukraine, 41 Nauki Prosp., 03028 Kyiv, Ukraine.
authors.forename: A S
authors.initials: AS
authors.lastname: Pavlyuchenko
authors.affiliation: Kyiv Clinic Hospital No.3, Kyiv Municipal Scientific and Practical Centre of Nephrology and Hemodialysis, 26 P. Zaporozhets str. Kyiv, Ukraine.
authors.forename: E K
authors.initials: EK
authors.lastname: Krasyuk
authors.affiliation: Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Zabolotnogo St., 03143 Kyiv, Ukraine.
authors.forename: A P
authors.initials: AP
authors.lastname: Soldatkin
authors.affiliation: Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Zabolotnogo St., 03143 Kyiv, Ukraine.
authors.forename: A V
authors.initials: AV
authors.lastname: El'skaya
mesh_terms: D015374:Biosensing Techniques / Q000379:methods* / Q000706:statistics & numerical data; D001826:Body Fluids / Q000737:chemistry; D003404:Creatinine / Q000032:analysis*; D006801:Humans; D007676:Kidney Failure, Chronic / Q000378:metabolism / Q000628:therapy; D006435:Renal Dialysis; D012680:Sensitivity and Specificity
chemical_list: D003404:Creatinine