title: Plasma ghrelin isoforms and gastric ghrelin O-acyltransferase expression are influenced by Helicobacter pylori status.
abstract: OBJECTIVE
  Helicobacter pylori is known to affect the host's nutritional status. This study was performed to elucidate the relationship between H. pylori status and the dynamics of the ghrelin system, in the context of ghrelin O-acyltransferase (GOAT) expression.
  
  
  METHODS
  We conducted a clinical study of 30 subjects focusing on the following points: 1) the effects of H. pylori infection on the concentrations of circulating ghrelin isoforms and on ghrelin and GOAT mRNA expression in the gastric mucosa, and 2) the effects of H. pylori eradication on the same parameters.
  
  
  RESULTS
  The plasma acyl-ghrelin and desacyl-ghrelin concentrations of 16 H. pylori positive participants were significantly lower than those of 14 H. pylori negative controls. The acyl-ghrelin/desacyl-ghrelin ratio was not significantly different between the H. pylori positive and H. pylori negative participants. The levels of ghrelin and GOAT mRNA in the gastric mucosa were significantly lower in the H. pylori positive participants than in the H. pylori negative controls. In 11 subjects in whom H. pylori eradication was successful, their plasma acyl-ghrelin levels tended to increase after H. pylori eradication, but the difference was not significant; however, their plasma desacyl-ghrelin levels were significantly reduced. Although gastric ghrelin mRNA expression increased significantly after H. pylori eradication, gastric GOAT mRNA expression tended to increase but was not significantly altered.
  
  
  CONCLUSIONS
  H. pylori status might affect the host's nutritional status through changes in the plasma levels of ghrelin isoforms and the gastric expression levels of ghrelin and GOAT mRNA.
authors.affiliation: Department of Gastroenterology, Social Insurance Kyoto Hospital, Kyoto, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Ando
authors.forename: Shigeto
authors.initials: S
authors.lastname: Mizuno
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Ishida
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Kondo
authors.forename: Ikuya
authors.initials: I
authors.lastname: Miki
authors.forename: Masaru
authors.initials: M
authors.lastname: Yoshida
authors.forename: Takeshi
authors.initials: T
authors.lastname: Azuma
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ishikawa
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Takagi
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Yagi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kokura
authors.forename: Yuji
authors.initials: Y
authors.lastname: Naito
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Yoshikawa
authors.forename: Akihiro
authors.initials: A
authors.lastname: Asakawa
authors.forename: Akio
authors.initials: A
authors.lastname: Inui
mesh_terms: D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism*; D000368:Aged; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism* / Q000382:microbiology; D054439:Ghrelin / Q000097:blood* / Q000235:genetics; D016481:Helicobacter Infections / Q000097:blood / Q000378:metabolism* / Q000382:microbiology; D016480:Helicobacter pylori; D006801:Humans; D008297:Male; D008875:Middle Aged; D020033:Protein Isoforms; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D054439:Ghrelin; D020033:Protein Isoforms; D012333:RNA, Messenger; D000217:Acyltransferases; C542776:MBOAT4 protein, human