title: Analysis of Panax notoginseng metabolites in rat bile by liquid chromatography-quadrupole time-of-flight mass spectrometry with microdialysis sampling.
abstract: A dynamic microdialysis sampling method with liquid chromatography-quadrupole time-of-flight mass spectrometry (Q-TOF-MS) was developed for rapid and sensitive analysis of the metabolite profile of Panax notoginseng extract (PNE) in rat bile. In vivo studies in male Sprague-Dawley rats were performed with microdialysis probes implanted into the bile duct before bile samples were collected from 0 to 12h. Metabolites of PNE were identified using dynamic adjustment of the fragmentor voltage to produce structure-relevant fragment ions. The mass accuracy of precursor and fragment ions was typically within 5 ppm of the theoretical values. We identified 7 compounds: 4 parent compounds (notoginsenoside R1, ginsenosides Rg1, Rb1, and Rd) and 3 metabolites (ginsenosides Rg2, Rh2, and compound K). Data from this study suggest that this microdialysis technique could be used in notoginseng saponin metabolic animal studies.
authors.affiliation: Tang Center for Herbal Medicine Research and Department of Anesthesia & Critical Care, Pritzker School of Medicine, The University of Chicago, 5841 South Maryland Avenue, Chicago, IL 60637, USA.
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Wen
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.forename: Rong-Hua
authors.initials: RH
authors.lastname: Ma
authors.forename: Wen
authors.initials: W
authors.lastname: Gao
authors.forename: Lian-Wen
authors.initials: LW
authors.lastname: Qi
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Brent A
authors.initials: BA
authors.lastname: Bauer
authors.forename: Guang-Jian
authors.initials: GJ
authors.lastname: Du
authors.forename: Zhiyu
authors.initials: Z
authors.lastname: Zhang
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Somogyi
authors.forename: Chong-Zhi
authors.initials: CZ
authors.lastname: Wang
authors.forename: Chun-Su
authors.initials: CS
authors.lastname: Yuan
mesh_terms: D000818:Animals; D001646:Bile / Q000737:chemistry* / Q000378:metabolism; D002853:Chromatography, Liquid / Q000379:methods*; D036145:Ginsenosides / Q000032:analysis* / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D017551:Microdialysis; D053723:Panax notoginseng; D010936:Plant Extracts / Q000008:administration & dosage / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D036145:Ginsenosides; D010936:Plant Extracts