title: Alternative pathway activation of complement in laparoscopic and open rectal surgery.
abstract: The study was designed to investigate whether complement is activated in patients subject to rectal surgery and whether the choice of surgical technique (open or laparoscopic) has any impact on the activation of complement. Our hypothesis is that laparoscopic surgery leads to a lower-level activation of complement than open surgery. Patients (n = 24) subject to rectal surgery owing to rectal cancer were included. The study was prospective and randomized. The patients were randomized to either laparoscopic surgery (n = 12) or open surgery (n = 12). Blood samples for determination of complement activation (C4d, Bb, C3bc and the terminal C5b-9 complex TCC) were drawn before start of surgery (T0) and at the following time-points after start of surgery: 180 min (T1), 360 min (T2), 24 h (T3) and 3-5 days (T4). A significant increase in the alternative pathway activation product Bb and in the terminal pathway activation product TCC was seen over time in both groups (P < 0.001). Bb peaked early (T1) and returned to baseline levels post-operatively, whereas TCC increased steadily with maximum values in the late post-operative period. The plasma concentrations of C4d and C3bc decreased significantly in both groups at T1 and T2 and returned to baseline levels at T4. There was no significant difference between the groups. Rectal surgery causes activation of the complement system. Complement is activated through the alternative pathway. Results mostly showed no significant differences between laparoscopic and open rectal surgery apart from lower levels of factor Bb in the former group in the perioperative period.
authors.affiliation: Department of Anaesthesiology & Intensive Care, Sahlgrenska University Hospital/East, Göteborg, Sweden. andreas.kvarnstrom@vgregion.se
authors.forename: A
authors.initials: A
authors.lastname: Kvarnström
authors.forename: A
authors.initials: A
authors.lastname: Sokolov
authors.forename: T
authors.initials: T
authors.lastname: Swartling
authors.forename: G
authors.initials: G
authors.lastname: Kurlberg
authors.forename: T E
authors.initials: TE
authors.lastname: Mollnes
authors.forename: A
authors.initials: A
authors.lastname: Bengtsson
mesh_terms: D000368:Aged; D003179:Complement C3b / Q000276:immunology / Q000378:metabolism; D015935:Complement C4b / Q000276:immunology; D015938:Complement Membrane Attack Complex / Q000276:immunology / Q000378:metabolism; D003170:Complement Pathway, Alternative / Q000276:immunology*; D013505:Digestive System Surgical Procedures / Q000379:methods*; D005260:Female; D006801:Humans; D010535:Laparoscopy / Q000379:methods*; D008297:Male; D008875:Middle Aged; D010446:Peptide Fragments / Q000097:blood / Q000276:immunology; D011446:Prospective Studies; D012004:Rectal Neoplasms / Q000276:immunology* / Q000601:surgery*; D018709:Statistics, Nonparametric
chemical_list: D015938:Complement Membrane Attack Complex; D010446:Peptide Fragments; D003179:Complement C3b; D015935:Complement C4b; C032261:complement C4d