title: A Streptococcus pneumoniae infection model in larvae of the wax moth Galleria mellonella.
abstract: The bacterium Streptococcus pneumoniae is a leading human opportunistic pathogen. The limitations of the current vaccine have led to increased recognition of the need to understand bacterial behaviour and competitive dynamics using in vivo models of infection. Here, we investigate the potential application of the larvae of the wax moth Galleria mellonella as an informative infection model. Larvae were challenged with a range of doses of S. pneumoniae isolates differing in known virulence factors to determine the LD(50) values. Infection dynamics were determined by obtaining bacterial counts from larvae over a time course. Differences in virulence between serotypes could be distinguished in this host. Infection with strains differing in known virulence factors demonstrated predicted differences in virulence. Acapsulate and pneumolysin-negative strains were less virulent than their respective wild types. A large reduction in virulence was seen in strains lacking cell wall D-alanylation. The mortality of G. mellonella larvae is attributable to bacterial growth within larvae, while surviving larvae are able to clear infections by reducing bacterial numbers. These data demonstrate that G. mellonella larvae represent an in vivo infection model with applications for investigating aspects of bacterial-host interactions such as the role of antimicrobial peptide activity and resistance.
authors.affiliation: Faculty of Life Sciences, University of Manchester, Michael Smith Building, Oxford Road, Manchester, M13 9PT, UK. benjamin_evans@hotmail.com
authors.forename: B A
authors.initials: BA
authors.lastname: Evans
authors.forename: D E
authors.initials: DE
authors.lastname: Rozen
mesh_terms: D000818:Animals; D058491:Bacterial Load; D001426:Bacterial Proteins / Q000235:genetics; D004195:Disease Models, Animal; D005798:Genes, Bacterial; D054884:Host-Pathogen Interactions; D007814:Larva / Q000382:microbiology; D007928:Lethal Dose 50; D009036:Moths / Q000382:microbiology*; D011008:Pneumococcal Infections / Q000382:microbiology*; D013045:Species Specificity; D013296:Streptococcus pneumoniae / Q000145:classification / Q000235:genetics / Q000472:pathogenicity*; D013301:Streptolysins / Q000235:genetics; D037521:Virulence Factors
chemical_list: D001426:Bacterial Proteins; D013301:Streptolysins; D037521:Virulence Factors; C032290:plY protein, Streptococcus pneumoniae