title: PTRF/cavin-1 and MIF proteins are identified as non-small cell lung cancer biomarkers by label-free proteomics.
abstract: With the completion of the human genome sequence, biomedical sciences have entered in the "omics" era, mainly due to high-throughput genomics techniques and the recent application of mass spectrometry to proteomics analyses. However, there is still a time lag between these technological advances and their application in the clinical setting. Our work is designed to build bridges between high-performance proteomics and clinical routine. Protein extracts were obtained from fresh frozen normal lung and non-small cell lung cancer samples. We applied a phosphopeptide enrichment followed by LC-MS/MS. Subsequent label-free quantification and bioinformatics analyses were performed. We assessed protein patterns on these samples, showing dozens of differential markers between normal and tumor tissue. Gene ontology and interactome analyses identified signaling pathways altered on tumor tissue. We have identified two proteins, PTRF/cavin-1 and MIF, which are differentially expressed between normal lung and non-small cell lung cancer. These potential biomarkers were validated using western blot and immunohistochemistry. The application of discovery-based proteomics analyses in clinical samples allowed us to identify new potential biomarkers and therapeutic targets in non-small cell lung cancer.
authors.affiliation: Laboratory of Molecular Pathology & Oncology, Instituto de Genética Médica y Molecular, Hospital Universitario La Paz, Madrid, Spain.
authors.forename: Angelo
authors.initials: A
authors.lastname: Gámez-Pozo
authors.forename: Iker
authors.initials: I
authors.lastname: Sánchez-Navarro
authors.forename: Enrique
authors.initials: E
authors.lastname: Calvo
authors.forename: María Teresa
authors.initials: MT
authors.lastname: Agulló-Ortuño
authors.forename: Rocío
authors.initials: R
authors.lastname: López-Vacas
authors.forename: Esther
authors.initials: E
authors.lastname: Díaz
authors.forename: Emilio
authors.initials: E
authors.lastname: Camafeita
authors.forename: Manuel
authors.initials: M
authors.lastname: Nistal
authors.forename: Rosario
authors.initials: R
authors.lastname: Madero
authors.forename: Enrique
authors.initials: E
authors.lastname: Espinosa
authors.forename: Juan Antonio
authors.initials: JA
authors.lastname: López
authors.forename: Juan Ángel
authors.initials: JÁ
authors.lastname: Fresno Vara
mesh_terms: D000595:Amino Acid Sequence; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D015153:Blotting, Western; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D053263:Gene Regulatory Networks; D006801:Humans; D007150:Immunohistochemistry; D019746:Intramolecular Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D008168:Lung / Q000378:metabolism / Q000473:pathology; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism*; D008263:Macrophage Migration-Inhibitory Factors / Q000235:genetics / Q000378:metabolism*; D013058:Mass Spectrometry; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010748:Phosphopeptides / Q000235:genetics / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D015203:Reproducibility of Results
chemical_list: D014408:Biomarkers, Tumor; C497835:CAVIN1 protein, human; D008263:Macrophage Migration-Inhibitory Factors; D010748:Phosphopeptides; D016601:RNA-Binding Proteins; D019746:Intramolecular Oxidoreductases; C515598:MIF protein, human