title: Burkholderia diazotrophica sp. nov., isolated from root nodules of Mimosa spp.
abstract: Five strains, JPY461(T), JPY359, JPY389, DPU-3 and STM4206 were isolated from nitrogen-fixing nodules on the roots of Mimosa spp. and their taxonomic positions were investigated using a polyphasic approach. All five strains grew at 15-40 °C (optimum, 30-37 °C), at pH 4.0-8.0 (optimum, pH 6.0-7.0) and with 0-1 % (w/v) NaCl [optimum, 0 % (w/v)]. On the basis of 16S rRNA gene sequence analysis, a representative strain (JPY461(T)) showed 97.2 % sequence similarity to the closest related species Burkholderia acidipaludis SA33(T), a similarity of 97.2 % to Burkholderia terrae KMY02(T), 97.1 % to Burkholderia phymatum STM815(T) and 97.1 % to Burkholderia hospita LMG 20598(T). The predominant fatty acids of the five novel strains were summed feature 2 (comprising C(16 : 1) iso I and/or C(14 : 0) 3-OH), summed feature 3 (comprising C(16 : 1)ω7c and/or C(16 : 1)ω6c), C(16 : 0) , C(16 : 0) 3-OH, C(17 : 0) cyclo, C(18 : 1)ω7c and C(19 : 0) cyclo ω8c. The major isoprenoid quinone was Q-8 and the DNA G+C content of the strains was 63.0-65.0 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an unidentified aminophospholipid, an unidentified aminolipid and several unidentified phospholipids. The DNA-DNA relatedness of the novel strain with respect to recognized species of the genus Burkholderia was less than 54 %. On the basis of 16S rRNA and recA gene sequence similarities, chemotaxonomic and phenotypic data, the five strains represent a novel species in the genus Burkholderia, for which the name Burkholderia diazotrophica sp. nov. is proposed with the type strain, JPY461(T) ( = LMG 26031(T) = BCRC 80259(T) = KCTC 23308(T)).
authors.affiliation: Department of Marine Biotechnology, National Kaohsiung Marine University, 142 Hai-Chuan Road, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
authors.forename: Shih-Yi
authors.initials: SY
authors.lastname: Sheu
authors.affiliation: Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, 142 Hai-Chuan Road, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
authors.forename: Jui-Hsing
authors.initials: JH
authors.lastname: Chou
authors.affiliation: Génétique et Microbiologie, UMR UHP-INRA 1128, IFR 110 EFABA, Université de Lorraine, Faculté des Sciences et Technologies, BP 239, 54506 Vandœuvre-lès-Nancy, France.
authors.forename: Cyril
authors.initials: C
authors.lastname: Bontemps
authors.affiliation: The James Hutton Institute, Craigiebuckler, Aberdeen AB15 8QH, UK.
authors.forename: Geoffrey N
authors.initials: GN
authors.lastname: Elliott
authors.affiliation: Depto. de Ciências Agrárias e Ambientais, Universidade Estadual de Santa Cruz, km 16, Ilhéus, 45662-900 BA, Brazil.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Gross
authors.affiliation: Embrapa Cerrados, Planaltina, 73301-970 DF, Brazil.
authors.forename: Fabio Bueno
authors.initials: FB
authors.lastname: Dos Reis Junior
authors.affiliation: IRD, UMR LSTM, 34060 Montpellier, France.
authors.forename: Rémy
authors.initials: R
authors.lastname: Melkonian
authors.affiliation: IRD, UMR LSTM, 34060 Montpellier, France.
authors.forename: Lionel
authors.initials: L
authors.lastname: Moulin
authors.affiliation: The James Hutton Institute, Invergowrie, Dundee DD2 5DA, UK.
authors.forename: Euan K
authors.initials: EK
authors.lastname: James
authors.affiliation: Division of Plant Sciences, University of Dundee at JHI, Invergowrie, Dundee DD2 5DA, UK.
authors.forename: Janet I
authors.initials: JI
authors.lastname: Sprent
authors.affiliation: Department of Biology, University of York, York YO10 5DD, UK.
authors.forename: J Peter W
authors.initials: JPW
authors.lastname: Young
authors.affiliation: Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, 142 Hai-Chuan Road, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
authors.forename: Wen-Ming
authors.initials: WM
authors.lastname: Chen
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D001938:Brazil; D019117:Burkholderia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005798:Genes, Bacterial; D031298:Mimosa / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D053204:Root Nodules, Plant / Q000382:microbiology*; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S