title: Multimodal optical imaging with multiphoton microscopy and optical coherence tomography.
abstract: Two types of combined multiphoton microscopy and optical coherence tomography (MPM/OCT) are compared for multimodal optical imaging. Single-scale multiphoton microscopy and optical coherence microscopy (MPM/OCM) is shown to acquire multiple contrasts from MPM and OCT simultaneously. Multi-scale MPM/OCT is shown to provide multiple field-of-views (FOVs), where OCT provides tissue level imaging and MPM provides cellular level imaging. In both types, the MPM includes two channels which are two-photon excited fluorescence (TPEF) and second harmonic generation (SHG). Representative images using each system are demonstrated on biological specimens. A detailed comparison of the two types of MPM/OCT shows that each system has its own pros and cons. MPM/OCM is high-resolution but with limited FOV, and OCM may or may not provide additional information than MPM depending on the samples. Multi-scale MPM/OCT can change FOV but need both low and high NA objectives. For future development, the two types of MPM/OCT can be further integrated to achieve both functions on a single system.
authors.affiliation: Department of Electrical and Computer Engineering, University of British Columbia, Vancouver, Canada. tang@ece.ubc.ca
authors.forename: Shuo
authors.initials: S
authors.lastname: Tang
authors.forename: Yifeng
authors.initials: Y
authors.lastname: Zhou
authors.forename: Myeong Jin
authors.initials: MJ
authors.lastname: Ju
mesh_terms: D006801:Humans; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*; D055620:Optical Phenomena; D041623:Tomography, Optical Coherence / Q000379:methods*