title: Glyoxalase-I is a novel prognosis factor associated with gastric cancer progression.
abstract: Glyoxalase I (GLO1), a methylglyoxal detoxification enzyme, is implicated in the progression of human malignancies. The role of GLO1 in gastric cancer development or progression is currently unclear. The expression of GLO1 was determined in primary gastric cancer specimens using quantitative polymerase chain reaction, immunohistochemistry (IHC), and western blotting analyses. GLO1 expression was higher in gastric cancer tissues, compared with that in adjacent noncancerous tissues. Elevated expression of GLO1 was significantly associated with gastric wall invasion, lymph node metastasis, and pathological stage, suggesting a novel role of GLO1 in gastric cancer development and progression. The 5-year survival rate of the lower GLO1 expression groups was significantly greater than that of the higher expression groups (log rank P = 0.0373) in IHC experiments. Over-expression of GLO1 in gastric cancer cell lines increases cell proliferation, migration and invasiveness. Conversely, down-regulation of GLO1 with shRNA led to a marked reduction in the migration and invasion abilities. Our data strongly suggest that high expression of GLO1 in gastric cancer enhances the metastasis ability of tumor cells in vitro and in vivo, and support its efficacy as a potential marker for the detection and prognosis of gastric cancer.
authors.affiliation: Department of Biochemistry, Chang Gung University, Taoyuan, Taiwan.
authors.forename: Wan-Li
authors.initials: WL
authors.lastname: Cheng
authors.forename: Ming-Ming
authors.initials: MM
authors.lastname: Tsai
authors.forename: Chung-Ying
authors.initials: CY
authors.lastname: Tsai
authors.forename: Ya-Hui
authors.initials: YH
authors.lastname: Huang
authors.forename: Cheng-Yi
authors.initials: CY
authors.lastname: Chen
authors.forename: Hsiang-Cheng
authors.initials: HC
authors.lastname: Chi
authors.forename: Yi-Hsin
authors.initials: YH
authors.lastname: Tseng
authors.forename: Im-Wai
authors.initials: IW
authors.lastname: Chao
authors.forename: Wei-Chi
authors.initials: WC
authors.lastname: Lin
authors.forename: Sheng-Ming
authors.initials: SM
authors.lastname: Wu
authors.forename: Ying
authors.initials: Y
authors.lastname: Liang
authors.forename: Chia-Jung
authors.initials: CJ
authors.lastname: Liao
authors.forename: Yang-Hsiang
authors.initials: YH
authors.lastname: Lin
authors.forename: I-Hsiao
authors.initials: IH
authors.lastname: Chung
authors.forename: Wei-Jan
authors.initials: WJ
authors.lastname: Chen
authors.forename: Paul Y
authors.initials: PY
authors.lastname: Lin
authors.forename: Chia-Siu
authors.initials: CS
authors.lastname: Wang
authors.forename: Kwang-Huei
authors.initials: KH
authors.lastname: Lin
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018450:Disease Progression; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007791:Lactoylglutathione Lyase / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D013270:Stomach / Q000201:enzymology / Q000473:pathology*; D013274:Stomach Neoplasms / Q000175:diagnosis* / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D015854:Up-Regulation
chemical_list: D007791:Lactoylglutathione Lyase