title: 68Ga-autoclabeling of DOTA-TATE and DOTA-NOC.
abstract: BACKGROUND
  A new method combining (68)Ga-labeling and steam sterilization, here called autoclabeling, has been evaluated for two somatostatin receptor binding tracers used for positron emission tomography (PET) imaging of neuroendocrine tumors; DOTA-TATE and -NOC.
  
  
  METHODS
  The two peptides DOTA-TATE and -NOC were labeled with (68)Ga by heating for 15 min at 121°C in the presence of acetate buffer at pH 4.3. The product solutions were tested for sterility, presence of endotoxins, degradation of peptide and osmolality.
  
  
  RESULTS
  Complete incorporation of (68)Ga was obtained after the autoclabeling reaction and no degradation of the peptides was observed. Sterility was verified and the presence of endotoxins was well within Ph. Eur limits (175IU/maximum injected volume).
  
  
  CONCLUSIONS
  The autoclabeling method provides a convenient procedure for (68)Ga-labeling by combining the labeling reaction and steam sterilization into one single step.
authors.affiliation: Department of Clinical Physiology, Herlev University Hospital, Herlev, Denmark.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Blom
authors.forename: Jacek
authors.initials: J
authors.lastname: Koziorowski
mesh_terms: D003287:Contrast Media / Q000138:chemical synthesis / Q000302:isolation & purification; D007553:Isotope Labeling / Q000379:methods*; D009942:Organometallic Compounds / Q000138:chemical synthesis* / Q000302:isolation & purification; D019275:Radiopharmaceuticals / Q000138:chemical synthesis* / Q000302:isolation & purification; D012371:Robotics / Q000379:methods*; D013227:Steam
chemical_list: C504894:68Ga-DOTANOC; D003287:Contrast Media; D009942:Organometallic Compounds; D019275:Radiopharmaceuticals; D013227:Steam; C513399:gallium Ga 68 dotatate