title: Analysis of Complex Protein Mixtures Using Nano-LC Coupled to MS/MS.
abstract: INTRODUCTIONNanoliter-LC coupled to tandem mass spectrometry (nano-LC-MS/MS) permits the rapid and sensitive determination of protein-protein interactions. By using a specific purification technique such as coimmunoprecipitation or affinity purification in conjunction with nano-LC-MS/MS, not only are proteins identified, but specific protein-protein interactions are elucidated as well.
authors.forename: David M
authors.initials: DM
authors.lastname: Schieltz
authors.forename: Michael P
authors.initials: MP
authors.lastname: Washburn
authors.forename: Lara G
authors.initials: LG
authors.lastname: Hays