title: MMP-2 and MMP-9 in normal mucosa are independently associated with outcome of colorectal cancer patients.
abstract: BACKGROUND
  Upregulation of the matrix metalloproteinases MMP-2 and MMP-9 in various cancers has been associated with worse survival of the patients.
  
  
  METHODS
  We assessed MMP-2 and MMP-9 levels in normal colorectal mucosa from colorectal cancer patients in relation to the course of the disease.
  
  
  RESULTS
  A high protein expression of MMP-2 as well as MMP-9 in normal mucosa was found to be correlated with worse 5-year survival. The combination of both parameters was an even stronger prognostic factor. These protein levels were found not to be related to the corresponding single nucleotide polymorphisms of MMP-2 (-1306C>T) and MMP-9 (-1562C>T). Multivariate analyses indicated that the MMP-2 and MMP-9 levels in normal mucosa are prognostic for survival, independent of TNM classification.
  
  
  CONCLUSIONS
  MMP-2 and MMP-9 levels in normal mucosa are indicative of the course of disease in colorectal cancer patients.
authors.affiliation: Department of Gastroenterology-Hepatology, Leiden University Medical Centre, The Netherlands. a.m.j.langers@lumc.nl
authors.forename: A M J
authors.initials: AM
authors.lastname: Langers
authors.forename: H W
authors.initials: HW
authors.lastname: Verspaget
authors.forename: L J A C
authors.initials: LJ
authors.lastname: Hawinkels
authors.forename: F J G M
authors.initials: FJ
authors.lastname: Kubben
authors.forename: W
authors.initials: W
authors.lastname: van Duijn
authors.forename: J J
authors.initials: JJ
authors.lastname: van der Reijden
authors.forename: J C H
authors.initials: JC
authors.lastname: Hardwick
authors.forename: D W
authors.initials: DW
authors.lastname: Hommes
authors.forename: C F M
authors.initials: CF
authors.lastname: Sier
mesh_terms: D000368:Aged; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000401:mortality*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism*; D009092:Mucous Membrane / Q000378:metabolism* / Q000473:pathology; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D011379:Prognosis; D011446:Prospective Studies; D012189:Retrospective Studies; D015996:Survival Rate
chemical_list: D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9