title: Spermidine affects the transcriptome responses to high temperature stress in ripening tomato fruit.
abstract: OBJECTIVE
  High temperature adversely affects quality and yield of tomato fruit. Polyamine can alleviate heat injury in plants. This study is aimed to investigate the effects of polyamine and high temperature on transcriptional profiles in ripening tomato fruit.
  
  
  METHODS
  An Affymetrix tomato microarray was used to evaluate changes in gene expression in response to exogenous spermidine (Spd, 1 mmol/L) and high temperature (33/27 °C) treatments in tomato fruits at mature green stage.
  
  
  RESULTS
  Of the 10101 tomato probe sets represented on the array, 127 loci were differentially expressed in high temperature-treated fruits, compared with those under normal conditions, functionally characterized by their involvement in signal transduction, defense responses, oxidation reduction, and hormone responses. However, only 34 genes were up-regulated in Spd-treated fruits as compared with non-treated fruits, which were involved in primary metabolism, signal transduction, hormone responses, transcription factors, and stress responses. Meanwhile, 55 genes involved in energy metabolism, cell wall metabolism, and photosynthesis were down-regulated in Spd-treated fruits.
  
  
  CONCLUSIONS
  Our results demonstrated that Spd might play an important role in regulation of tomato fruit response to high temperature during ripening stage.
authors.affiliation: Key Laboratory of Horticultural Plant Growth, Development and Biotechnology, Ministry of Agriculture, Department of Horticulture, Zhejiang University, Hangzhou 310029, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Cheng
authors.forename: Rong-rong
authors.initials: RR
authors.lastname: Sun
authors.forename: Fei-yan
authors.initials: FY
authors.lastname: Wang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Peng
authors.forename: Fu-ling
authors.initials: FL
authors.lastname: Kong
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
authors.forename: Jia-shu
authors.initials: JS
authors.lastname: Cao
authors.forename: Gang
authors.initials: G
authors.lastname: Lu
mesh_terms: D005638:Fruit / Q000187:drug effects / Q000502:physiology*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D018869:Heat-Shock Response / Q000187:drug effects / Q000502:physiology*; D006358:Hot Temperature; D018551:Solanum lycopersicum / Q000187:drug effects / Q000502:physiology*; D013095:Spermidine / Q000494:pharmacology*; D059467:Transcriptome / Q000187:drug effects / Q000502:physiology*
chemical_list: D013095:Spermidine