title: Mucosal imbalance of interleukin-1β and interleukin-1 receptor antagonist in canine inflammatory bowel disease.
abstract: Interleukin (IL)-1β is a key mediator of the inflammatory response. IL-1 receptor antagonist (IL-1Ra) regulates inflammation by functioning as an endogenous inhibitor of IL-1β. A disruption of the balance between IL-1β and IL-1Ra has been identified in human inflammatory bowel disease (IBD). The objective of this study was to determine whether there is an intestinal imbalance of IL-1β and IL-1Ra in canine IBD by comparing expression of IL-1β and IL-1Ra mRNA by real-time RT-PCR and expression of IL-1β and IL-1Ra protein by ELISA in 21 dogs with IBD, 15 dogs with intestinal lymphoma ('inflammatory' controls) and 20 healthy Beagles ('healthy' controls). A significant decrease in the intestinal IL-1Ra:IL-1β ratio of mRNA and protein was observed in IBD cases when compared with healthy control dogs. In contrast, a decrease in IL-1Ra:IL-1β ratio was not observed in dogs with intestinal lymphoma. The IL-1Ra:IL-1β protein ratio was negatively correlated with clinical severity in dogs with IBD. An intestinal imbalance between IL-1β and IL-1Ra production may play a role in the pathogenesis of canine IBD.
authors.affiliation: Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Maeda
authors.forename: Koichi
authors.initials: K
authors.lastname: Ohno
authors.forename: Kenji
authors.initials: K
authors.lastname: Nakamura
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Uchida
authors.forename: Ko
authors.initials: K
authors.lastname: Nakashima
authors.forename: Kenjiro
authors.initials: K
authors.lastname: Fukushima
authors.forename: Atsushi
authors.initials: A
authors.lastname: Tsukamoto
authors.forename: Yuko
authors.initials: Y
authors.lastname: Goto-Koshino
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Fujino
authors.forename: Hajime
authors.initials: H
authors.lastname: Tsujimoto
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000378:metabolism*; D004285:Dogs; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology; D015212:Inflammatory Bowel Diseases / Q000378:metabolism / Q000662:veterinary*; D053590:Interleukin 1 Receptor Antagonist Protein / Q000235:genetics / Q000378:metabolism*; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D053590:Interleukin 1 Receptor Antagonist Protein; D053583:Interleukin-1beta; D012333:RNA, Messenger