title: A novel water-soluble β-D-glucan isolated from the spores of Ganoderma lucidum.
abstract: Ganoderma lucidum is an edible and medicinal mushroom used widely in East Asia. In recent years, its spores have been used as a supplement in combination with other forms of antitumor therapies. The cell wall of Ganoderma lucidum spores contains a high amount of polysaccharides. In this study, a neutral polysaccharide, GLSA50-1B, was isolated from sporoderm-broken spores of Ganoderma lucidum, by hot-water extraction, graded ethanol precipitation, anion-exchange chromatography, and gel permeation chromatography. Using sugar compositional analysis, methylation analysis, partial acid hydrolysis, acetolysis, and NMR and ESI-MS spectroscopy, GLSA50-1B was elucidated to be a novel β-D-glucan featured by a 1,6-linked β-D-Glcp backbone with different length of branches consisting of terminal and 1,4-linked Glcp residues, attached to O-4 of alternative Glc residues in the backbone.
authors.affiliation: Glycobiology and Glycochemistry Lab., Shanghai Institute of Materia Medica, Chinese Academy of Sciences and Infinitus, Shanghai 201203, China. qdong@mail.shcnc.ac.cn
authors.forename: Qun
authors.initials: Q
authors.lastname: Dong
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Shi
authors.forename: Jian
authors.initials: J
authors.lastname: Yao
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Fangli
authors.initials: F
authors.lastname: Ma
authors.forename: Kan
authors.initials: K
authors.lastname: Ding
mesh_terms: D002240:Carbohydrate Sequence; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000737:chemistry; D020940:Reishi / Q000737:chemistry*; D012995:Solubility; D013172:Spores, Fungal / Q000737:chemistry*; D014867:Water / Q000737:chemistry*; D047071:beta-Glucans / Q000737:chemistry*
chemical_list: D011134:Polysaccharides; D047071:beta-Glucans; D014867:Water