title: RUNX3 mediates suppression of tumor growth and metastasis of human CCRCC by regulating cyclin related proteins and TIMP-1.
abstract: Here we presented that the expression of RUNX3 was significantly decreased in 75 cases of clear cell renal cell carcinoma (CCRCC) tissues (p<0.05). Enforced RUNX3 expression mediated 786-O cells to exhibit inhibition of growth, G1 cell-cycle arrest and metastasis in vitro, and to lost tumorigenicity in nude mouse model in vivo. RUNX3-induced growth suppression was found partially to regulate various proteins, including inhibition of cyclinD1, cyclinE, cdk2, cdk4 and p-Rb, but increase of p27(Kip1), Rb and TIMP-1. Therefore, RUNX3 had the function of inhibiting the proliferative and metastatic abilities of CCRCC cells by regulating cyclins and TIMP1.
authors.affiliation: Department of Nephrology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: Lijie
authors.initials: L
authors.lastname: He
authors.forename: Xiaodi
authors.initials: X
authors.lastname: Zhao
authors.forename: Hanmin
authors.initials: H
authors.lastname: Wang
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.forename: Changcun
authors.initials: C
authors.lastname: Guo
authors.forename: Chen
authors.initials: C
authors.lastname: Huang
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Liu
authors.forename: Fangfang
authors.initials: F
authors.lastname: Yao
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.forename: Weijuan
authors.initials: W
authors.lastname: Lou
authors.forename: Shiren
authors.initials: S
authors.lastname: Sun
authors.forename: Daiming
authors.initials: D
authors.lastname: Fan
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002292:Carcinoma, Renal Cell / Q000378:metabolism / Q000473:pathology*; D002455:Cell Division / Q000502:physiology; D050677:Core Binding Factor Alpha 3 Subunit / Q000502:physiology*; D016213:Cyclins / Q000502:physiology*; D005434:Flow Cytometry; D006801:Humans; D007680:Kidney Neoplasms / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008819:Mice, Nude; D009362:Neoplasm Metastasis; D060888:Real-Time Polymerase Chain Reaction; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000502:physiology*
chemical_list: D050677:Core Binding Factor Alpha 3 Subunit; D016213:Cyclins; C493729:Runx3 protein, human; C560822:TIMP1 protein, human; D019715:Tissue Inhibitor of Metalloproteinase-1