title: Solution structure of the natively assembled yeast ribosomal stalk determined by small-angle X-ray scattering.
abstract: The ribosomal stalk of the 60S subunit has been shown to play a crucial role in all steps of protein synthesis, but its structure and exact molecular function remain an unanswered question. In the present study, we show the low-resolution models of the solution structure of the yeast ribosomal stalk, composed of five proteins, P0-(P1-P2)(2). The model of the pentameric stalk complex determined by small-angle X-ray scattering reveals an elongated shape with a maximum length of 13 nm. The model displays three distinct lobes, which may correspond to the individual P1-P2 heterodimers anchored to the C-terminal domain of the P0 protein.
authors.affiliation: Department of Molecular Biology, Maria Curie-Sklodowska University, Lublin, Poland.
authors.forename: Przemysław
authors.initials: P
authors.lastname: Grela
authors.forename: Michal J
authors.initials: MJ
authors.lastname: Gajda
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Armache
authors.forename: Roland
authors.initials: R
authors.lastname: Beckmann
authors.forename: Dawid
authors.initials: D
authors.lastname: Krokowski
authors.forename: Dmitri I
authors.initials: DI
authors.lastname: Svergun
authors.forename: Nikodem
authors.initials: N
authors.lastname: Grankowski
authors.forename: Marek
authors.initials: M
authors.lastname: Tchórzewski
mesh_terms: D011485:Protein Binding / Q000502:physiology; D055503:Protein Multimerization; D017434:Protein Structure, Tertiary; D012269:Ribosomal Proteins / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000737:chemistry; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry*; D053838:Scattering, Small Angle; D014961:X-Ray Diffraction / Q000379:methods
chemical_list: C056002:RPP0 protein, S cerevisiae; D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins; C054675:ribosomal protein P0