title: Amelioration of oxidative stress in bio-membranes and macromolecules by non-toxic dye from Morinda tinctoria (Roxb.) roots.
abstract: Plant dyes have been in use for coloring and varied purposes since prehistoric times. A red dye found in the roots of plants belonging to genus Morinda is a well recognized coloring ingredient. The dye fraction obtained from the methanolic extract of the roots of Morinda tinctoria was explored for its role in attenuating damages caused by H(2)O(2)-induced oxidative stress. The antioxidant potential of the dye fraction was assessed through DPPH radical scavenging, deoxyribose degradation and inhibition of lipid peroxidation in mice liver. It was subsequently screened for its efficiency in extenuating damage incurred to biomembrane (using erythrocytes and their ghost membranes) and macromolecules (pBR322 DNA, lipids and proteins) from exposure to hydrogen peroxide. In addition, the non-toxic nature of the dye was supported by the histological evaluation conducted on the tissue sections from the major organs of Swiss Albino mice as well as effect on Hep3B cell line (human hepatic carcinoma). The LC-MS confirms the dye fraction to be morindone. Our study strongly suggests that morindone present in the root extracts of M. tinctoria, in addition to being a colorant, definitely holds promise in the pharmaceutical industry.
authors.affiliation: Division of Plant Biotechnology, School of Bio Sciences and Technology, VIT University, Vellore 632014, Tamil Nadu, India.
authors.forename: Dipita
authors.initials: D
authors.lastname: Bhakta
authors.forename: Ramamoorthy
authors.initials: R
authors.lastname: Siva
mesh_terms: D000818:Animals; D001713:Biphenyl Compounds / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004396:Coloring Agents / Q000302:isolation & purification / Q000494:pharmacology*; D004247:DNA / Q000187:drug effects; D003855:Deoxyribose / Q000737:chemistry; D004910:Erythrocyte Membrane / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008055:Lipids / Q000737:chemistry; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008566:Membranes / Q000378:metabolism*; D051379:Mice; D032066:Morinda / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects*; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D011506:Proteins / Q000737:chemistry; D053719:Tandem Mass Spectrometry
chemical_list: D001713:Biphenyl Compounds; D004396:Coloring Agents; D016166:Free Radical Scavengers; D008055:Lipids; D010851:Picrates; D010936:Plant Extracts; D011506:Proteins; D003855:Deoxyribose; D004247:DNA; C004931:1,1-diphenyl-2-picrylhydrazyl