title: Characterization of D-lactate dehydrogenase producing D-3-phenyllactic acid from Pediococcus pentosaceus.
abstract: D-Lactate dehydrogenase (D-LDH) from Pediococcus pentosaceus ATCC 25745 was found to produce D-3-phenyllactic acid from phenylpyruvate. The optimum pH and temperature for enzyme activity were pH 5.5 and 45 °C. The Michaelis-Menten constant (K(m)), turnover number (k(cat)), and catalytic efficiency (k(cat)/K(m)) values for the substrate phenylpyruvate were estimated to be 1.73 mmol/L, 173 s(-1), and 100 (mmol/L)(-1) s(-1) respectively.
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, China.
authors.forename: Shuhuai
authors.initials: S
authors.lastname: Yu
authors.forename: Houyi
authors.initials: H
authors.lastname: Jiang
authors.forename: Bo
authors.initials: B
authors.lastname: Jiang
authors.forename: Wanmeng
authors.initials: W
authors.lastname: Mu
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002413:Cations, Divalent; D003001:Cloning, Molecular; D004926:Escherichia coli; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D045022:Lactate Dehydrogenases / Q000235:genetics / Q000378:metabolism*; D007773:Lactates / Q000378:metabolism*; D019216:Metals, Heavy / Q000378:metabolism; D010376:Pediococcus / Q000737:chemistry / Q000201:enzymology*; D010667:Phenylpyruvic Acids / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D002413:Cations, Divalent; D007773:Lactates; D019216:Metals, Heavy; D010667:Phenylpyruvic Acids; D011994:Recombinant Proteins; C017648:3-phenyllactic acid; D045022:Lactate Dehydrogenases; C071195:D-lactate dehydrogenase; C031606:phenylpyruvic acid