title: Escape mutants of pandemic influenza A/H1N1 2009 virus: variations in antigenic specificity and receptor affinity of the hemagglutinin.
abstract: A panel of 6 neutralizing monoclonal antibodies (MAbs) raised against A/Moscow/IIV01/2009 (H1N1) virus isolated during the 2009 pandemic was used for the selection of 26 escape mutants. The mutants were characterized in immune cross-reactions with the panel of MAbs. The sequencing of the mutant HA genes revealed 5 amino acid positions recognized by monoclonal antibodies: 129, 156, 158, 159, and 190 (H3 numbering). The amino acid positions were distributed in two epitopes belonging to antigenic sites Sa and Sb. The mutant HAs exhibited variations in the affinity to synthetic high molecular mass sialic acid-containing receptor analogues. Results are discussed in connection with the antigenic drift potential of the "swine-like" pandemic 2009 influenza virus.
authors.affiliation: D.I. Ivanovsky Institute of Virology, Gamaleya Str. 16, Moscow 123098, Russia. irinarydneva@gmail.com
authors.forename: Irina
authors.initials: I
authors.lastname: Rudneva
authors.forename: Anna
authors.initials: A
authors.lastname: Ignatieva
authors.forename: Tatiana
authors.initials: T
authors.lastname: Timofeeva
authors.forename: Aleksandr
authors.initials: A
authors.lastname: Shilov
authors.forename: Alla
authors.initials: A
authors.lastname: Kushch
authors.forename: Olga
authors.initials: O
authors.lastname: Masalova
authors.forename: Regina
authors.initials: R
authors.lastname: Klimova
authors.forename: Nicolai
authors.initials: N
authors.lastname: Bovin
authors.forename: Larisa
authors.initials: L
authors.lastname: Mochalova
authors.forename: Nikolai
authors.initials: N
authors.lastname: Kaverin
mesh_terms: D057134:Antibodies, Neutralizing / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology; D004252:DNA Mutational Analysis; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000276:immunology*; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D007251:Influenza, Human / Q000821:virology; D008969:Molecular Sequence Data; D009031:Moscow; D050505:Mutant Proteins / Q000235:genetics / Q000276:immunology*; D020125:Mutation, Missense; D012641:Selection, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D050505:Mutant Proteins