title: Impact of a mutator phenotype on motility and cell adherence in Salmonella Heidelberg.
abstract: In this study, we investigated adherence and motility of the hypermutator Salmonella enterica Heidelberg B182 bovine strain related to a 12bp deletion in mutS. This mutator phenotype was associated with increased adherence to epithelial cells and with high expression of fimA as shown by real-time RT-PCR. Motility studies showed that fliC were up-regulated in the B182 strain, while fljA and fljB were down-regulated. In order to determine if mutated mutS is implicated in this genes expression, isogenic strains, derived from a WT strain, containing the 12bp deletion in mutS (Δ12bpmutS) or an inactivated mutS (ΔmutS) were generated. Δ12bpmutS and ΔmutS strains showed a spontaneous mutation rate similar to the environmental strain B182, but exhibited lower adherence capacity and fimA expression. In contrast to the fimbriae genes, in Δ12bpmutS, fliC expression was up-regulated, but fljA and fljB expression were decreased, as in the B182 strain. Only fljB expression was increased in ΔmutS mutants. Taken together, our data suggest that mutS alteration does not influence fimbriae expression but can impact flagella genes.
authors.affiliation: Equipe Microbiologie, UPRES-EA 1254, IFR GFAS 140, Université Européenne de Bretagne, Faculté des Sciences Pharmaceutiques et Biologiques, Université de Rennes I, 2 Avenue du Professeur Léon Bernard, 35043 Rennes, France.
authors.forename: Hervé
authors.initials: H
authors.lastname: Le Bars
authors.forename: Sandrine
authors.initials: S
authors.lastname: Le Gall-David
authors.forename: Virginie Madeleine
authors.initials: VM
authors.lastname: Renoux
authors.forename: Martine
authors.initials: M
authors.lastname: Bonnaure-Mallet
authors.forename: Anne
authors.initials: A
authors.lastname: Jolivet-Gougeon
authors.forename: Latifa
authors.initials: L
authors.lastname: Bousarghin
mesh_terms: D001422:Bacterial Adhesion / Q000235:genetics*; D010861:Fimbriae, Bacterial / Q000235:genetics; D005407:Flagella / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D006367:HeLa Cells; D006801:Humans; D051719:MutS DNA Mismatch-Binding Protein / Q000235:genetics / Q000378:metabolism; D010641:Phenotype; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019779:Salmonella enterica / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
chemical_list: D051719:MutS DNA Mismatch-Binding Protein