title: Definitive setup of clinical scale procedure for ex vivo expansion of cord blood hematopoietic cells for transplantation.
abstract: We recently developed a clinical grade ex vivo cord blood expansion procedure enabling a massive amplification of hematopoietic progenitors without any loss of stem cell potential. This procedure, based on day 14 liquid cultures of cord blood CD34(+) cells, in medium Macopharma HP01 and in the presence of stem cell factor (SCF; 100 ng/ml), fms-related tyrosine kinase 3-ligand (Flt-3L; 100 ng/ml), megakaryocyte growth and developmental factor (MGDF; 100 ng/ml), and granulocyte colony-stimulating factor (G-CSF; 10 ng/ml) had to be modified due to the commercially unavailability of clinical grade MGDF molecule. So MGDF was replaced by thrombopoietin (TPO) in fivefold lower dose (20 ng/ml), and culture time was reduced to 12 days. That way, a mean expansion fold of 400, 80, and 150 was obtained for total cells, CD34(+) cells, and colony-forming cells (CFCs), respectively. This amplification was associated with a slight enhancing effect on stem cells [Scid repopulating cells (SRCs)]. These are the ultimate preclinical modifications of a clinical grade expansion protocol, which is already employed in an ongoing clinical trial.
authors.affiliation: Etablissement Français du Sang Aquitaine-Limousin, 5 Place Amélie Raba Léon, Bordeaux, France.
authors.forename: Pascale
authors.initials: P
authors.lastname: Duchez
authors.forename: Jean
authors.initials: J
authors.lastname: Chevaleyre
authors.forename: Marija
authors.initials: M
authors.lastname: Vlaski
authors.forename: Bernard
authors.initials: B
authors.lastname: Dazey
authors.forename: Noel
authors.initials: N
authors.lastname: Milpied
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Boiron
authors.forename: Zoran
authors.initials: Z
authors.lastname: Ivanovic
mesh_terms: D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology*; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006801:Humans