title: Noninvasive MRI measurement of CBF: evaluating an arterial spin labelling sequence with 99mTc-HMPAO CBF autoradiography in a rat stroke model.
abstract: Arterial spin labelling (ASL) is increasingly available for noninvasive cerebral blood flow (CBF) measurement in stroke research. Here, a pseudo-continuous ASL technique (pCASL) was evaluated against (99m)Tc-D, L-hexamethylpropyleneamine oxime ((99m)Tc-HMPAO) autoradiography in a rat stroke model. The (99m)Tc-HMPAO was injected (intravenously, 225 MBq) during pCASL acquisition. The pCASL and (99m)Tc-HMPAO autoradiography CBF measures, relative to the contralateral hemisphere, were in good agreement across the spectrum of flow values in normal and ischemic tissues. The pCASL-derived quantitative regional CBF values (contralateral: 157 to 177 mL/100 g per minute; ipsilateral: 9 to 104 mL/100 g per minute) were consistent with the literature values. The data show the potential utility of pCASL for CBF assessment in a rat stroke model.
authors.affiliation: Glasgow Experimental MRI Centre, Institute of Neuroscience and Psychology, University of Glasgow, Glasgow, Scotland.
authors.forename: Tracey A
authors.initials: TA
authors.lastname: Baskerville
authors.forename: Christopher
authors.initials: C
authors.lastname: McCabe
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Weir
authors.forename: I Mhairi
authors.initials: IM
authors.lastname: Macrae
authors.forename: William M
authors.initials: WM
authors.lastname: Holmes
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000000981:diagnostic imaging* / Q000503:physiopathology; D002560:Cerebrovascular Circulation; D004195:Disease Models, Animal; D018810:Magnetic Resonance Angiography / Q000379:methods*; D011859:Radiography; D019275:Radiopharmaceuticals / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013113:Spin Labels; D020521:Stroke / Q000000981:diagnostic imaging* / Q000503:physiopathology; D019690:Technetium Tc 99m Exametazime / Q000494:pharmacology*
chemical_list: D019275:Radiopharmaceuticals; D013113:Spin Labels; D019690:Technetium Tc 99m Exametazime