title: Synthesis and characterization of ratiometric nanosensors for pH quantification: a mixed micelle approach.
abstract: Optical nanoparticle pH sensors designed for ratiometric measurements have previously been synthesized using post-functionalization approaches to introduce sensor molecules and to modify nanoparticle surface chemistry. This strategy often results in low control of the nanoparticle surface chemistry and is prone to batch-to-batch variations, which is undesirable for succeeding sensor calibrations and cellular measurements. Here we provide a new synthetic approach for preparing nanoparticle pH sensors based on self-organization principles, which in comparison to earlier strategies offers a much higher design flexibility and high control of particle size, morphology and surface chemistry.
authors.affiliation: Technical University of Denmark, Department for Micro- and Nanotechnology, DTU Nanotech, 2800 Lyngby, Denmark.
authors.forename: E K Pramod
authors.initials: EK
authors.lastname: Kumar
authors.forename: Kristoffer
authors.initials: K
authors.lastname: Almdal
authors.forename: Thomas L
authors.initials: TL
authors.lastname: Andresen
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008823:Micelles; D046529:Microscopy, Electron, Transmission; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D005456:Fluorescent Dyes; D008823:Micelles