title: Central corneal thickness considered an index of corneal hydration of the UVB irradiated rabbit cornea as influenced by UVB absorber.
abstract: UVB radiation from sunlight induces an acute corneal inflammation, photokeratitis, accompanied by changes in corneal hydration. We employed a method of ultrasonic pachymetry for daily examination of central corneal thickness as an index of corneal hydration of the rabbit cornea repeatedly irradiated by UVB radiation (312 nm, daily dose of 0.25 J/cm(2) during three or four days) as influenced by UVB absorber (actinoquinol combined with hyaluronic acid) dropped on the ocular surface during irradiation. One day after the third irradiation procedure the animals were sacrificed and corneas examined immuno-histochemically for peroxynitrite formation, a marker of oxidative damage, the antioxidant aldehyde dehydrogenase 3A1 and endothelial nitric oxide synthase, an enzyme generated nitric oxide. Results show that UV absorber combined with hyaluronic acid protected the cornea against UVB-induced changes in corneal thickness and microscopical disturbances to the cornea (both seen after buffered saline application) until the fourth experimental day. These UVB doses are equivalent to a daily exposure of 2.5 hrs of the human cornea to solar UVB radiation for three consecutive days. It is suggested that actinoquinol/ hyaluronic acid drops might be helpful for the human eye in the defence against photooxidative and other oxidative processes.
authors.affiliation: Institute of Experimental Medicine, Prague, Czech Republic. cejkova@biomed.cas.cz
authors.forename: C
authors.initials: C
authors.lastname: Cejka
authors.forename: J
authors.initials: J
authors.lastname: Luyckx
authors.forename: J
authors.initials: J
authors.lastname: Cejková
mesh_terms: D060433:Administration, Ophthalmic; D000444:Aldehyde Dehydrogenase / Q000378:metabolism; D000818:Animals; D001834:Body Water / Q000378:metabolism; D003315:Cornea / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000378:metabolism; D015715:Corneal Edema / Q000000981:diagnostic imaging / Q000378:metabolism / Q000517:prevention & control*; D063171:Corneal Pachymetry; D019610:Cytoprotection; D006820:Hyaluronic Acid / Q000008:administration & dosage* / Q000737:chemistry; D007150:Immunohistochemistry; D007634:Keratitis / Q000000981:diagnostic imaging / Q000378:metabolism / Q000517:prevention & control*; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D030421:Peroxynitrous Acid / Q000378:metabolism; D011804:Quinolines / Q000008:administration & dosage* / Q000737:chemistry; D011817:Rabbits; D011833:Radiation Injuries, Experimental / Q000000981:diagnostic imaging / Q000378:metabolism / Q000517:prevention & control*; D011837:Radiation-Protective Agents / Q000008:administration & dosage* / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors; D014463:Ultrasonography; D014466:Ultraviolet Rays
chemical_list: D011804:Quinolines; D011837:Radiation-Protective Agents; D030421:Peroxynitrous Acid; C556636:actinoquinol; D006820:Hyaluronic Acid; D052250:Nitric Oxide Synthase Type III; D000444:Aldehyde Dehydrogenase