title: CdSe and ZnSe quantum dots capped with PEA for screening C-reactive protein in human serum.
abstract: A fluorescence chemical sensor for C-reactive protein (CRP) was developed based on the selective interaction with CdSe and ZnSe quantum dots (QDs) coated with O-phosphorylethanolamine (PEA). Synthesis procedure and analytical parameters such as pH and ionic strength were studied. The decrease in the fluorescence emission intensity was explained due to the specific interaction of the QDs-PEA with CRP, and a correlation was observed between the quenching of the fluorescence and the concentration of CRP. The accuracy of the proposed method was 0.37% as RSD. The proposed method was applied to screen serum samples, and showed to be sensible at the C-reactive protein concentrations of risks levels.
authors.affiliation: Centro de Investigação em Química, Departamento de Química e Bioquímica, Faculdade de Ciências da Universidade do Porto, Rua do Campo Alegre 687, 4169-007 Porto, Portugal.
authors.forename: D
authors.initials: D
authors.lastname: Gomes
authors.forename: M
authors.initials: M
authors.lastname: Algarra
authors.forename: M J
authors.initials: MJ
authors.lastname: Diez de los Rios
authors.forename: M M
authors.initials: MM
authors.lastname: Arrebola
authors.forename: M E
authors.initials: ME
authors.lastname: Herrera-Gutiérrez
authors.forename: G
authors.initials: G
authors.lastname: Seller-Pérez
authors.forename: J C G
authors.initials: JC
authors.lastname: Esteves da Silva
mesh_terms: D001774:Blood Chemical Analysis / Q000379:methods*; D002097:C-Reactive Protein / Q000032:analysis* / Q000378:metabolism; D019187:Cadmium Compounds / Q000737:chemistry*; D002138:Calibration; D004983:Ethanolamines / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D009994:Osmolar Concentration; D045663:Quantum Dots; D018036:Selenium Compounds / Q000737:chemistry*; D017967:Zinc Compounds / Q000737:chemistry*
chemical_list: D019187:Cadmium Compounds; D004983:Ethanolamines; D008024:Ligands; D018036:Selenium Compounds; D017967:Zinc Compounds; C005448:phosphorylethanolamine; D002097:C-Reactive Protein; C058667:cadmium selenide; C044696:zinc selenide