title: Micropropagation of Cyclopia genistoides, an endemic South African plant of economic importance.
abstract: An efficient micropropagation protocol of Cyclopia genistoides (L.) Vent., an indigenous South African shrub of economic importance, was established. In vitro shoot cultures were obtained from shoot tip fragments of sterile seedlings cultured on solid Schenk and Hildebrandt (SH) medium supplemented with 9.84 microM 6-(gamma,gamma-dimethylallylamino)purine (2iP) and 1.0 microM thidiazuron (TDZ). Maximum shoot multiplication rate [(8.2 +/- 1.3) microshoots/explant)] was observed on this medium composition. Prior to rooting, the multiplied shoots were elongated for 60 days (two 30-days passages) on SH medium with one-half sucrose concentration, supplemented with 4.92 microM indole-3-butyric acid (IBA). The rooting of explants was only possible in the case of the elongated shoots. The highest root induction rate (54.8%) was achieved on solid SH medium with one-half sucrose and one-half potassium nitrate and ammonium nitrate concentration, respectively, supplemented with 28.54 microM indole-3-acetic acid (IAA) and 260.25 microM citric acid. The plantlets were acclimatized for 30 days in the glasshouse, with the use of peat/gravel/perlite substrate (1:1:1). The highest acclimatization rate (80%) was obtained for explants rooted with the use of IAA-supplemented medium. The phytochemical profile of the regenerated plants was similar to that of the reference intact plant material. HPLC analyses showed that C. genistoides plantlets obtained by the micropropagation procedure kept the ability to produce xanthones (mangiferin and isomangiferin) and the flavanone hesperidin, characteristic of wild-growing shrubs.
authors.affiliation: The Chair and Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Gdansk, al. gen. J. Hallera 107, 80-416 Gdansk, Poland.
authors.forename: Adam
authors.initials: A
authors.lastname: Kokotkiewicz
authors.forename: Maria
authors.initials: M
authors.lastname: Luczkiewicz
authors.forename: Anna
authors.initials: A
authors.lastname: Hering
authors.forename: Renata
authors.initials: R
authors.lastname: Ochocka
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Gorynski
authors.forename: Adam
authors.initials: A
authors.lastname: Bucinski
authors.forename: Pawel
authors.initials: P
authors.lastname: Sowinski
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D053998:Cyclopia Plant / Q000254:growth & development*; D018517:Plant Roots / Q000254:growth & development; D018520:Plant Shoots / Q000254:growth & development; D013019:South Africa; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D003470:Culture Media