title: Photoactivation-based labeling and in vivo tracking of RNA molecules in the nucleus.
abstract: This protocol describes a method for observing and measuring the movement of RNA molecules in the nucleus of living mammalian cells. Caged fluorescein-labeled DNA oligonucleotides are introduced into living mammalian cells, where they demonstrably hybridize to complementary RNA. After site-specific photoactivation at desired sites within the cell, the RNA movements away from those sites are followed and digitally recorded using a rapid acquisition microscopy system.
authors.forename: Joan C Ritland
authors.initials: JC
authors.lastname: Politz
authors.forename: Richard A
authors.initials: RA
authors.lastname: Tuft
authors.forename: Thoru
authors.initials: T
authors.lastname: Pederson