title: Absorption, disposition, metabolism, and excretion of [3-(14)C]caffeic acid in rats.
abstract: Male Sprague-Dawley rats ingested 140 × 10(6) dpm of [3-(14)C]trans-caffeic acid, and over the ensuing 72 h period, body tissues, plasma, urine, and feces were collected and the overall levels of radioactivity determined. Where sufficient radioactivity had accumulated, samples were analyzed by HPLC with online radioactivity and tandem mass spectrometric detection. Nine labeled compounds were identified, the substrate and its cis isomer, 3'-O- and 4'-O-sulfates and glucuronides of caffeic acid, 4'-O-sulfates and glucuronides of ferulic acid, and isoferulic acid-4'-O-sulfate. Four unidentified metabolites were also detected. After passing down the gastrointestinal tract, the majority of the radiolabeled metabolites were excreted in urine with minimal accumulation in plasma. Only relatively small amounts of an unidentified (14)C-labeled metabolite were expelled in feces. There was little or no accumulation of radioactivity in body tissues, including the brain. The overall recovery of radioactivity 72 h after ingestion of [3-(14)C]caffeic acid was ∼80% of intake.
authors.affiliation: Plant Products and Human Nutrition Group, School of Medicine, Joseph Black Building,  University of Glasgow , Glasgow G12 8QQ, United Kingdom.
authors.forename: Maizatul H
authors.initials: MH
authors.lastname: Omar
authors.forename: William
authors.initials: W
authors.lastname: Mullen
authors.forename: Angelique
authors.initials: A
authors.lastname: Stalmach
authors.forename: Cyril
authors.initials: C
authors.lastname: Auger
authors.forename: Jean-Max
authors.initials: JM
authors.lastname: Rouanet
authors.forename: Pierre-Louis
authors.initials: PL
authors.lastname: Teissedre
authors.forename: Stuart T
authors.initials: ST
authors.lastname: Caldwell
authors.forename: Richard C
authors.initials: RC
authors.lastname: Hartley
authors.forename: Alan
authors.initials: A
authors.lastname: Crozier
mesh_terms: D000042:Absorption; D000818:Animals; D001682:Biological Availability; D002109:Caffeic Acids / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D002250:Carbon Radioisotopes; D002851:Chromatography, High Pressure Liquid; D005243:Feces / Q000737:chemistry; D041981:Gastrointestinal Tract / Q000737:chemistry / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry; D014018:Tissue Distribution
chemical_list: D002109:Caffeic Acids; D002250:Carbon Radioisotopes; C040048:caffeic acid