title: Establishment of rapid and specific real-time PCR assays for the detection of hepatitis B viral genotype in tibet.
abstract: Hepatitis B virus (HBV) with a C/D recombinant genotype is dominant in Tibet. A rapid, simple and specific genotyping system, consisting of three real-time PCR assays, was developed for the determination of genotypes C and D as well as two forms of recombinant C/D. Overall, 95% of the 22 samples were genotyped accurately. This system is considered to be a useful method for large-scale genotype surveys in Tibet.
authors.affiliation: Department of Genetics, Shanghai-MOST Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Center at Shanghai, Shanghai 201203, China.
authors.forename: Jinxiu
authors.initials: J
authors.lastname: Shi
authors.lastname: Basangzhuoma
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Xing
authors.lastname: Yangla
authors.forename: Chaoying
authors.initials: C
authors.lastname: Cui
mesh_terms: D005838:Genotype; D006509:Hepatitis B / Q000821:virology*; D006515:Hepatitis B virus / Q000145:classification* / Q000235:genetics*; D006801:Humans; D017720:Molecular Epidemiology / Q000379:methods; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D011995:Recombination, Genetic; D018609:Tibet; D014773:Virology / Q000379:methods*