title: A nomenclature for intestinal in vitro cultures.
abstract: Many advances have been reported in the long-term culture of intestinal mucosal cells in recent years. A significant number of publications have described new culture media, cell formations, and growth patterns. Furthermore, it is now possible to study, e.g., the capabilities of isolated stem cells or the interactions between stem cells and mesenchyme. However, at the moment there is significant variation in the way these structures are described and named. A standardized nomenclature would benefit the ability to communicate and compare findings from different laboratories using the different culture systems. To address this issue, members of the NIH Intestinal Stem Cell Consortium herein propose a systematic nomenclature for in vitro cultures of the small and large intestine. We begin by describing the structures that are generated by preparative steps. We then define and describe structures produced in vitro, specifically: enterosphere, enteroid, reconstituted intestinal organoid, induced intestinal organoid, colonosphere, colonoid, and colonic organoid.
authors.affiliation: UCLA/VA Greater Los Angeles, 11301 Wilshire Blvd. (10H2) Los Angeles, CA 90073, USA. stelzner@ucla.edu
authors.forename: Matthias
authors.initials: M
authors.lastname: Stelzner
authors.forename: Michael
authors.initials: M
authors.lastname: Helmrath
authors.forename: James C Y
authors.initials: JC
authors.lastname: Dunn
authors.forename: Susan J
authors.initials: SJ
authors.lastname: Henning
authors.forename: Courtney W
authors.initials: CW
authors.lastname: Houchen
authors.forename: Calvin
authors.initials: C
authors.lastname: Kuo
authors.forename: John
authors.initials: J
authors.lastname: Lynch
authors.forename: Linheng
authors.initials: L
authors.lastname: Li
authors.forename: Scott T
authors.initials: ST
authors.lastname: Magness
authors.forename: Martin G
authors.initials: MG
authors.lastname: Martin
authors.forename: Melissa H
authors.initials: MH
authors.lastname: Wong
authors.forename: Jian
authors.initials: J
authors.lastname: Yu
mesh_terms: D018929:Cell Culture Techniques / Q000145:classification*; D003106:Colon / Q000166:cytology*; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology; D007421:Intestine, Small / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology; D009626:Terminology as Topic