title: Cotranscriptional role for Arabidopsis DICER-LIKE 4 in transcription termination.
abstract: Transcription termination is emerging as an important component of gene regulation necessary to partition the genome and minimize transcriptional interference. We have discovered a role for the Arabidopsis RNA silencing enzyme DICER-LIKE 4 (DCL4) in transcription termination of an endogenous Arabidopsis gene, FCA. DCL4 directly associates with FCA chromatin in the 3' region and promotes cleavage of the nascent transcript in a domain downstream of the canonical polyA site. In a dcl4 mutant, the resulting transcriptional read-through triggers an RNA interference-mediated gene silencing of a transgene containing the same 3' region. We conclude that DCL4 promotes transcription termination of the Arabidopsis FCA gene, reducing the amount of aberrant RNA produced from the locus.
authors.affiliation: Department of Cell and Developmental Biology, John Innes Centre, Norwich Research Park, Norwich, UK.
authors.forename: Fuquan
authors.initials: F
authors.lastname: Liu
authors.forename: Saleha
authors.initials: S
authors.lastname: Bakht
authors.forename: Caroline
authors.initials: C
authors.lastname: Dean
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002843:Chromatin / Q000235:genetics / Q000378:metabolism; D047369:Chromatin Immunoprecipitation; D018506:Gene Expression Regulation, Plant; D026161:MADS Domain Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D026723:Polyadenylation; D017434:Protein Structure, Tertiary; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics* / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D043244:Ribonuclease III / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D019076:Transgenes
chemical_list: D029681:Arabidopsis Proteins; D002843:Chromatin; C418902:At5g10140 protein, Arabidopsis; C106535:Flowering time control protein FCA, Arabidopsis; D026161:MADS Domain Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D016601:RNA-Binding Proteins; C504982:DCL4 protein, Arabidopsis; D043244:Ribonuclease III