title: Identification of a potential biomarker panel for the intake of the common dietary trans fat elaidic acid (trans∆9-C18:1).
abstract: Trans fatty acid intake has been correlated to an unfavorable plasma lipoprotein profile and an increased cardiovascular disease risk. The present study aimed to identify a plasma protein biomarker panel related to human intake of elaidic acid. The human liver cell line HepG2-SF was used as a model system, and the cells were maintained for seven days in serum-free medium containing 100 μM elaidic acid (trans∆9-C18:1), oleic acid (cis∆9-C18:1) or stearic acid (C18:0). The secretomes were analyzed by stable isotope labeling of amino acids in cell culture (SILAC), difference in gel electrophoresis (DIGE) and gene expression microarray analysis. Twelve proteins were found to be differentially regulated based on SILAC data (>1.3 fold change, P-value<0.05), 13 proteins were found to be differentially regulated based on DIGE analysis (>1.3 fold change, P-value<0.05), and 17 mRNA transcripts encoding extracellular proteins were determined to be affected (>1.3 fold change, P-value<0.01) following the addition of elaidic acid compared to oleic acid or stearic acid. The results revealed that 37 proteins were regulated specifically in response to elaidic acid exposure, and nine of these proteins were confirmed to be regulated in this manner by using selected reaction monitoring mass spectrometry.
authors.affiliation: Center for Insoluble Protein Structure at the Department of Molecular Biology, University of Aarhus, Denmark.
authors.forename: Toke Peter
authors.initials: TP
authors.lastname: Krogager
authors.forename: Lone Vendel
authors.initials: LV
authors.lastname: Nielsen
authors.forename: Steffen
authors.initials: S
authors.lastname: Bak
authors.forename: Clifford
authors.initials: C
authors.lastname: Young
authors.forename: Carla
authors.initials: C
authors.lastname: Ferreri
authors.forename: Ole Nørregaard
authors.initials: ON
authors.lastname: Jensen
authors.forename: Peter
authors.initials: P
authors.lastname: Højrup
authors.forename: Vladimiros
authors.initials: V
authors.lastname: Thoma
authors.forename: Ida B
authors.initials: IB
authors.lastname: Thøgersen
authors.forename: Jan J
authors.initials: JJ
authors.lastname: Enghild
mesh_terms: D015415:Biomarkers / Q000032:analysis*; D002250:Carbon Radioisotopes; D003470:Culture Media; D004041:Dietary Fats / Q000008:administration & dosage*; D004591:Electrophoresis, Polyacrylamide Gel; D056945:Hep G2 Cells; D006801:Humans; D008099:Liver / Q000378:metabolism; D019301:Oleic Acid / Q000008:administration & dosage* / Q000378:metabolism; D009829:Oleic Acids; D020411:Oligonucleotide Array Sequence Analysis; D011506:Proteins / Q000302:isolation & purification*; D012333:RNA, Messenger / Q000378:metabolism; D013229:Stearic Acids / Q000378:metabolism; D053719:Tandem Mass Spectrometry; D044242:Trans Fatty Acids / Q000008:administration & dosage*
chemical_list: D015415:Biomarkers; D002250:Carbon Radioisotopes; D003470:Culture Media; D004041:Dietary Fats; D009829:Oleic Acids; D011506:Proteins; D012333:RNA, Messenger; D013229:Stearic Acids; D044242:Trans Fatty Acids; D019301:Oleic Acid; C011459:elaidic acid; C031183:stearic acid