title: Fractionation of sulphite spent liquor for biochemical processing using ion exchange resins.
abstract: Sulphite spent liquor (SSL) is a side product from acidic sulphite pulping of wood, which organic counterpart is composed mainly by lignosulphonates (LS) and sugars. The last are a prominent substrate for the bioprocessing although a previous purification step is necessary to eliminate microbial inhibitors. In this study a fractionation of hardwood SSL (HSSL) has been accomplished employing ion exchange resins in order to separate sugars fraction from concomitant inhibitors: LS, acetic acid, furan derivatives, phenolics, acetic acid and excess of inorganic salts. The fractionation of HSSL has been carried out using two fixed-bed ion exchangers in series (cationic+anionic). The first cation exchange column packed with Dowex 50WX2 resin was able to eliminate free cations and partially separate sugars from high molecular weight LS and furan derivatives. The second anion exchange column packed with Amberlite IRA-96 sorbed remaining LS, phenolics and acetic acid. Overall, the series arrangement under investigation has removed 99.99% of Mg(2+), 99.0% of Ca(2+), 99.6% of LS, and 100% of acetic acid, whereas the yield of recovered sugars was at least 72% of their total amount in HSSL.
authors.affiliation: CICECO, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal.
authors.forename: D L A
authors.initials: DL
authors.lastname: Fernandes
authors.forename: C M
authors.initials: CM
authors.lastname: Silva
authors.forename: A M R B
authors.initials: AM
authors.lastname: Xavier
authors.forename: D V
authors.initials: DV
authors.lastname: Evtuguin
mesh_terms: D056804:Biofuels; D001709:Biotechnology / Q000379:methods; D002241:Carbohydrates / Q000737:chemistry / Q000302:isolation & purification; D002412:Cations / Q000737:chemistry; D005591:Chemical Fractionation / Q000379:methods*; D005052:Eucalyptus; D005285:Fermentation; D007475:Ion Exchange Resins / Q000737:chemistry*; D008031:Lignin / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification; D013447:Sulfites / Q000737:chemistry*; D014934:Wood
chemical_list: D056804:Biofuels; D002241:Carbohydrates; D002412:Cations; D007475:Ion Exchange Resins; D013447:Sulfites; C001545:lignosulfuric acid; D008031:Lignin