title: Rat sperm immobilisation effects of a protein from Ricinus communis (Linn.): an in vitro comparative study with nonoxynol-9.
abstract: Previous study conducted in our department showed that 50% ethanolic extract of the root of Ricinus communis possess reversible antifertility effect and a 62-kDa protein (Rp) from this extract is responsible for the antifertility effects. In this study, we compared the spermicidal effect of this Rp with nonoxynol-9 (N-9) in vitro. The sperm immobilisation studies showed that 100 μg ml(-1)  of Rp was able to immobilise the sperms completely within 30 s. Sperm revival test revealed that the spermicidal effect was irreversible. There was also a significant reduction in sperm viability and hypo-osmotic swelling in Rp and N-9 treated groups in comparison with the control. In Rp and N-9 treated groups, the number of acrosome-reacted cells was found to be high and also caused agglutination of the spermatozoa, indicating the loss of intactness of the plasma membrane, which was further supported by the significant reduction in the activity of membrane bound 5'-nucleotidase, acrosomal acrosin. In short, the protein Rp possesses spermicidal activity in vitro and its effects are similar to that of nonoxynol 9.
authors.affiliation: Department of Biochemistry, University of Kerala, Kariavattom, Kerala, India.
authors.forename: R S
authors.initials: RS
authors.lastname: Nithya
authors.forename: M M
authors.initials: MM
authors.lastname: Anuja
authors.forename: C
authors.initials: C
authors.lastname: Rajamanickam
authors.forename: M
authors.initials: M
authors.lastname: Indira
mesh_terms: D015720:5'-Nucleotidase / Q000378:metabolism; D000176:Acrosin / Q000378:metabolism; D020101:Acrosome Reaction / Q000187:drug effects; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D008297:Male; D008970:Molecular Weight; D017137:Nonoxynol / Q000494:pharmacology*; D010940:Plant Proteins / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D051381:Rats; D017207:Rats, Sprague-Dawley; D012278:Ricinus / Q000737:chemistry*; D013073:Sperm Agglutination / Q000187:drug effects; D013078:Sperm Immobilizing Agents / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000166:cytology / Q000187:drug effects / Q000502:physiology
chemical_list: D010940:Plant Proteins; D013078:Sperm Immobilizing Agents; D017137:Nonoxynol; D015720:5'-Nucleotidase; D000176:Acrosin