title: Enzymatic synthesis of 8-vinyl- and 8-styryl-2'-deoxyguanosine modified DNA--novel fluorescent molecular probes.
abstract: Fluorescent analogs of the natural nucleobases are widely used as molecular probes for investigating DNA hybridization and topology. In this study the guanosine analogs 8-vinyl- and 8-styryl-2'-deoxyguanosine were synthesized and converted into the corresponding 5'-triphosphates. These C8 modified nucleotides were processed by various DNA polymerases to create fluorescent DNA. Whereas the 8-styryl modified nucleotide somewhat hampers DNA synthesis 8-vinyl-2'-deoxyguanosine is processed by DNA polymerases emphasizing the broad applicability as a molecular probe for fluorescence spectroscopy.
authors.affiliation: Department of Chemistry and Konstanz Research School Chemical Biology, University of Konstanz, 78457 Konstanz, Germany.
authors.forename: Bastian
authors.initials: B
authors.lastname: Holzberger
authors.forename: Julian
authors.initials: J
authors.lastname: Strohmeier
authors.forename: Vanessa
authors.initials: V
authors.lastname: Siegmund
authors.forename: Ulf
authors.initials: U
authors.lastname: Diederichsen
authors.forename: Andreas
authors.initials: A
authors.lastname: Marx
mesh_terms: D004247:DNA / Q000737:chemistry*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D003849:Deoxyguanosine / Q000031:analogs & derivatives*; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D014753:Vinyl Compounds / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D014753:Vinyl Compounds; D004247:DNA; D004259:DNA-Directed DNA Polymerase; D003849:Deoxyguanosine