title: Phytochemical studies of the phenolic substances in Aster glehni extract and its sedative and anticonvulsant activity.
abstract: On high performance liquid chromatography, the caffeoylquinic acid (CQ) occupying the highest proportion of the water-ethanol (7:3) extract of Aster glehni (Compositae) leaves was 3-Op-coumaroylquinic acid (46.10 ± 4.22 mg/g of dried weight) among CQs tested. The IC₅₀ of the water-ethanol (7:3) extract was 4.23 ± 0.24 μg/mL in the peroxynitrite (ONOO(-))-scavenging assay. Phytochemical isolation from A. glehni extract yielded three kaempferol glycosides. The water-ethanol (7:3) extract and both p-coumaric acid and caffeic acid, phenylpropanoid moieties of CQs, had sedative effects in pentobarbital-induced sleeping time in mice and anticonvulsant effects in pentylenetetrazole-induced mice. Furthermore, the phenolic substance-rich W-E (7:3) extract of A. glehni could be used to treat anxiety or convulsion partly due to its peroxynitrite-scavenging mechanism.
authors.affiliation: Department of Agro-industrial Technology, Faculty of Agriculture, Lambung Mangkurat University, Indonesia 70714.
authors.forename: Agung
authors.initials: A
authors.lastname: Nugroho
authors.forename: Myung-Hoe
authors.initials: MH
authors.lastname: Kim
authors.forename: Jongwon
authors.initials: J
authors.lastname: Choi
authors.forename: Jae Sue
authors.initials: JS
authors.lastname: Choi
authors.forename: Won Tae
authors.initials: WT
authors.lastname: Jung
authors.forename: Kyung-Tae
authors.initials: KT
authors.lastname: Lee
authors.forename: Hee-Juhn
authors.initials: HJ
authors.lastname: Park
mesh_terms: D000818:Animals; D000927:Anticonvulsants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D031173:Aster Plant / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004195:Disease Models, Animal; D000431:Ethanol / Q000737:chemistry; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006993:Hypnotics and Sedatives / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D044949:Kaempferols / Q000302:isolation & purification / Q000494:pharmacology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010424:Pentobarbital / Q000494:pharmacology; D010433:Pentylenetetrazole; D030421:Peroxynitrous Acid / Q000737:chemistry; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves; D011801:Quinic Acid / Q000031:analogs & derivatives / Q000302:isolation & purification / Q000494:pharmacology; D012640:Seizures / Q000139:chemically induced / Q000517:prevention & control*; D012890:Sleep / Q000187:drug effects*; D012997:Solvents / Q000737:chemistry; D013997:Time Factors; D014867:Water / Q000737:chemistry
chemical_list: D000927:Anticonvulsants; D016166:Free Radical Scavengers; D006993:Hypnotics and Sedatives; D044949:Kaempferols; D010636:Phenols; D010936:Plant Extracts; D012997:Solvents; C472707:caffeoylquinic acid; D011801:Quinic Acid; D014867:Water; D030421:Peroxynitrous Acid; D000431:Ethanol; D010424:Pentobarbital; D010433:Pentylenetetrazole