title: CD47(high) expression on CD4 effectors identifies functional long-lived memory T cell progenitors.
abstract: T cell memory is the hallmark of adaptive immunity. Central questions are to determine which cells among proliferating effector T cells will live beyond the crash of the immune response (IR) and develop into functional memory T cells. CD47, considered as a marker of self, is implicated in cell death, cell elimination, and in the inflammatory response. We report in this article that CD47 expression was transiently regulated on Ag-specific CD4 T cells, that is, from CD47(high) to CD47(low) to CD47(high), during the course of the in vivo IR. Specifically, CD47(high) status marked central memory CD4 T cell precursors at an early time point of the IR. By contrast, cytokine production was a functional attribute restricted to CD47(high), but not CD47(low), polyclonal effector CD4 T cells during recall responses in an experimental model of chronic airway inflammatory disease. Passive transfer of CD47(high), but not CD47(low), CD4 T cells in nonlymphopenic naive mice generated long-lived memory T cells capable of anamnestic responses. We conclude that CD47(high) status on CD4 T cells identifies functional long-lived memory T cell progenitors.
authors.affiliation: Laboratoire d'Immunorégulation, Centre de Recherche du Hospitalier de l'Université de Montréal, l'Hôpital Notre-Dame, Montréal, Québec H2L 4M1, Canada.
authors.forename: Vu Quang
authors.initials: VQ
authors.lastname: Van
authors.forename: Marianne
authors.initials: M
authors.lastname: Raymond
authors.forename: Nobuyasu
authors.initials: N
authors.lastname: Baba
authors.forename: Manuel
authors.initials: M
authors.lastname: Rubio
authors.forename: Keiko
authors.initials: K
authors.lastname: Wakahara
authors.forename: Santos A
authors.initials: SA
authors.lastname: Susin
authors.forename: Marika
authors.initials: M
authors.lastname: Sarfati
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D051928:CD47 Antigen / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D005786:Gene Expression Regulation / Q000502:physiology*; D007111:Immunity, Cellular / Q000502:physiology*; D007156:Immunologic Memory / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D054504:Precursor Cells, T-Lymphoid / Q000166:cytology / Q000276:immunology* / Q000378:metabolism
chemical_list: D051928:CD47 Antigen; C497895:Cd47 protein, mouse