title: WWOX-mediated apoptosis in A549 cells mainly involves the mitochondrial             pathway.
abstract: The human WWOX gene, known as WW domain-containing oxidoreductase, is located             on 16q23.3-24.1, a chromosome region that spans the common fragile site, FRA16D.             Abnormal transcripts or even loss of expression are frequently found in a number             of cancer cell types, including breast, ovarian, prostate and lung cancer cells.             It has therefore been proposed that the WWOX gene encodes a candidate tumor suppressor,             possibly a pro-apoptotic protein. However, the mechanism behind this is not entirely             clear. In the present study, we examined the pro-apoptotic action of WWOX using             transient expression in A549 cells. We observed that the ectopic expression of             WWOX caused apoptosis in A549 cells. We further observed procaspase-3 and procaspase-9             activation and the release of cytochrome C from the mitochondria in A549 cells             transfected with pcDNA3.0-WWOX. These data indicate that WWOX induces apoptosis             in A549 cells via the mitochondrial pathway.
authors.affiliation: Department of Biochemistry and Molecular Biology, Shanghai Jiaotong University School of Medicine, Shanghai, PR China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.forename: Renbing
authors.initials: R
authors.lastname: Jia
authors.forename: Lei
authors.initials: L
authors.lastname: Ying
authors.forename: Bo
authors.initials: B
authors.lastname: Liu
authors.forename: Guanxiang
authors.initials: G
authors.lastname: Qian
authors.forename: Xianqun
authors.initials: X
authors.lastname: Fan
authors.forename: Shengfang
authors.initials: S
authors.lastname: Ge
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism; D000077192:Adenocarcinoma of Lung; D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D045304:Cytochromes c / Q000378:metabolism; D004789:Enzyme Activation / Q000235:genetics; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism*; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*; D000074584:WW Domain-Containing Oxidoreductase
chemical_list: D025521:Tumor Suppressor Proteins; D045304:Cytochromes c; D010088:Oxidoreductases; D000074584:WW Domain-Containing Oxidoreductase; C486071:WWOX protein, human; D053148:Caspase 3; D053453:Caspase 9