title: Regulated interaction of protein phosphatase 1 and protein phosphatase 2A with phospholipase C-related but catalytically inactive protein.
abstract: Protein phosphatase 1 (PP1) and protein phosphatase 2A (PP2A) are major members of the protein serine/threonine phosphatase families. We have identified PP1 and PP2A as interacting partners of PRIP (phospholipase C-related but catalytically inactive protein), a protein isolated in our laboratory. We first investigated the interaction of PRIP with two phosphatases, using purified recombinant proteins. PRIP immobilized on beads pulled down the catalytic subunits of both PP1 and PP2A, indicating that the interactions were in a direct manner, and the binding of PP1 and the binding of PP2A to PRIP were mutually exclusive. Site-directed mutagenesis experiments revealed that the binding sites for PP1 and PP2A on PRIP were not identical, but similar. Phosphorylation of PRIP by protein kinase A (PKA) resulted in the weakened binding of PP1, but not PP2A. Rather, the dissociation of PP1 from PRIP by phosphorylation accompanied the strengthened binding of PP2A in in vitro experiments. This regulation of binding of PP1 and PP2A to PRIP by PKA-dependent phosphorylation was also observed in living cells treated with forskolin or isoproterenol. These results suggested that PRIP directly interacts with the catalytic subunits of two distinct phosphatases in a mutually exclusive manner and the interactions are regulated by phosphorylation, thus functioning as a scaffold to regulate the activities and subcellular localizations of both PP1 and PP2A in phospho-dependent cellular signaling.
authors.affiliation: Laboratory of Molecular and Cellular Biochemistry, Kyushu University, Fukuoka 812-8582, Japan.
authors.forename: Goro
authors.initials: G
authors.lastname: Sugiyama
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takeuchi
authors.forename: Koki
authors.initials: K
authors.lastname: Nagano
authors.forename: Jing
authors.initials: J
authors.lastname: Gao
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Ohyama
authors.forename: Yoshihide
authors.initials: Y
authors.lastname: Mori
authors.forename: Masato
authors.initials: M
authors.lastname: Hirata
mesh_terms: D000818:Animals; D001665:Binding Sites; D019556:COS Cells; D020134:Catalytic Domain; D002522:Chlorocebus aethiops; D016297:Mutagenesis, Site-Directed; D010766:Phosphorylation; D054645:Protein Phosphatase 1 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D054648:Protein Phosphatase 2 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D014162:Transfection
chemical_list: D054645:Protein Phosphatase 1; D054648:Protein Phosphatase 2