title: Gene and protein expression signature of endometrial glandular and stromal compartments during the window of implantation.
abstract: OBJECTIVE
  To map the changes in messenger RNA (mRNA) and protein abundance during the window of implantation in specifically endometrial stromal and glandular epithelial cells obtained using laser microdissection microscopy (LDM).
  
  
  METHODS
  Endometrial samples were collected from two menstrual cycles at 2 and 7 days after first significant rise in blood LH, and separate cell populations were obtained using LDM. A new generation linear polymerase chain reaction (PCR) amplified the mRNA, which were hybridized to both Affymetrix U133 Plus2 and Agilent 4x44K microarrays followed by gene set analysis. Immunohistochemistry assessed protein expression between the two collection times.
  
  
  METHODS
  In vitro fertilization clinic.
  
  
  METHODS
  Nine Caucasian, fertile, cycling women.
  
  
  METHODS
  None.
  
  
  METHODS
  Cycle dating using blood markers; microarrays on laser microdissected glands and stroma; dual platform microarray confirmation; immunohistochemical analysis of cell cycle proteins.
  
  
  RESULTS
  The two microarray platforms showed concordance. During the window of implantation, a statistically significant network of 22 mRNA associated with the cell cycle was down-regulated. Immunohistochemistry identified altered localization in stroma.
  
  
  CONCLUSIONS
  Microarrays demonstrated glands and stroma have distinct mRNA signatures, each dependent on the day of the cycle. We characterized two compartments of the receptive endometrium with a transcriptomic signature identifying regulation of only the cell cycle. Immunohistochemical analysis of cell cycle proteins identified a signature staining pattern of nuclear relocalization of a group of cyclins of stromal cells, which may be clinically applicable.
authors.affiliation: Department of Obstetrics and Gynaecology, University of Otago, Christchurch, New Zealand. gloria.evans@otago.ac.nz
authors.forename: Gloria E
authors.initials: GE
authors.lastname: Evans
authors.forename: José A
authors.initials: JA
authors.lastname: Martínez-Conejero
authors.forename: Gregory T M
authors.initials: GT
authors.lastname: Phillipson
authors.forename: Carlos
authors.initials: C
authors.lastname: Simón
authors.forename: Les A
authors.initials: LA
authors.lastname: McNoe
authors.forename: Peter H
authors.initials: PH
authors.lastname: Sykes
authors.forename: José A
authors.initials: JA
authors.lastname: Horcajadas
authors.forename: Enid Y N
authors.initials: EY
authors.lastname: Lam
authors.forename: Cristin G
authors.initials: CG
authors.lastname: Print
authors.forename: Iris L
authors.initials: IL
authors.lastname: Sin
authors.forename: John J
authors.initials: JJ
authors.lastname: Evans
mesh_terms: D000328:Adult; D010064:Embryo Implantation / Q000235:genetics*; D004717:Endometrium / Q000166:cytology / Q000502:physiology*; D004847:Epithelial Cells / Q000502:physiology*; D005260:Female; D005298:Fertility / Q000235:genetics; D005307:Fertilization in Vitro; D015870:Gene Expression / Q000502:physiology; D006801:Humans; D008183:Luteal Phase / Q000235:genetics; D007986:Luteinizing Hormone / Q000097:blood; D020411:Oligonucleotide Array Sequence Analysis; D017154:Stromal Cells / Q000502:physiology*; D055815:Young Adult
chemical_list: D007986:Luteinizing Hormone