title: The 1st International standard for transforming growth factor-β3 (TGF-β3).
abstract: One candidate preparation of human sequence recombinant transforming growth factor-β3 (TGF-β3) was formulated and lyophilized at NIBSC prior to evaluation in a collaborative study for its suitability to serve as an international standard. The preparation was tested by 8 laboratories using in vitro bioassays and immunoassays. The candidate preparation 09/234 was judged suitable to serve as an international standard based on the data obtained for biological activity and stability. On the basis of the results reported here, the preparation coded 09/234 was established by the WHO Expert Committee on Biological Standardisation (ECBS) as the WHO 1st IS for human TGF-β3 with an assigned value for TGF-β3 activity of 19,000 IU/ampoule.
authors.affiliation: Biotherapeutics Group, NIBSC, Blanche Lane, South Mimms, Potters Bar, Hertfordshire, EN6 3QG, UK. Meenu.Wadhwa@nibsc.hpa.org.uk
authors.forename: Meenu
authors.initials: M
authors.lastname: Wadhwa
authors.forename: Paula
authors.initials: P
authors.lastname: Dilger
authors.forename: Michelle
authors.initials: M
authors.lastname: Hamill
authors.forename: David
authors.initials: D
authors.lastname: Bending
authors.forename: Sarah
authors.initials: S
authors.lastname: Gibbs
authors.forename: Guoping
authors.initials: G
authors.lastname: Wu
authors.forename: Jo
authors.initials: J
authors.lastname: Read
authors.forename: Tony
authors.initials: T
authors.lastname: Wyss-Coray
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: John
authors.initials: J
authors.lastname: Little
authors.forename: Katherine M
authors.initials: KM
authors.lastname: Getliffe
authors.forename: Gao
authors.initials: G
authors.lastname: Kai
authors.forename: Weihong
authors.initials: W
authors.lastname: Wang
authors.forename: David
authors.initials: D
authors.lastname: Bender
authors.forename: Chris
authors.initials: C
authors.lastname: Bird
authors.forename: Alan B
authors.initials: AB
authors.lastname: Heath
authors.forename: Anne
authors.initials: A
authors.lastname: Cooke
authors.forename: Robin
authors.initials: R
authors.lastname: Thorpe
mesh_terms: D001681:Biological Assay / Q000379:methods / Q000592:standards; D006801:Humans; D007118:Immunoassay / Q000379:methods / Q000592:standards; D012015:Reference Standards; D053782:Transforming Growth Factor beta3 / Q000592:standards*
chemical_list: C509044:TGFB3 protein, human; D053782:Transforming Growth Factor beta3