title: [Biological behaviour of colon cancer cells transfected with  C-erbB2 shRNA plasmid].
abstract: OBJECTIVE
  To study the changes of the cell growth,  cell cycle distribution and cell apoptosis of colon cancer cell, HT-29, when C-erbB2 gene was knockdown by shRNA against C-erbB2.
  
  
  METHODS
  Cell growth, cell cycle distribution and cell apoptosis were compared among three groups including plasmid experimental group(PEG), transfected reagent control group(TRCG)and negative plasmid control group(NPCG). Cell growth was measured by MTT assay. Cell cycle distribution and cell apoptosis were detected by flow cytometry.
  
  
  RESULTS
  The inhibition rate of cell growth of PEG, TRCG and NPCG were 39.65%, 7.23% and 8.05%  respectively. The cell growth was significantly inhibited in PEG(P<0.01). The cells of G0/G1 phase were 74.93%, 67.19%,  68.05%  respectively in PEG, TRCG and NPCG. The cells of G0/G1 phase in PEG were significantly more than those in TRCG and NPCG(P<0.05).While the cells of S phase were 7.81%, 14.02%, 13.70% in PEG, TRCG and NPCG respectively. The cells of S phase in PEG were significantly less than those in TRCG or NPCG(P<0.05).The cell apoptosis rate were 19.21%, 3.13%, 4.08% in PEG, TRCG and NPCG respectively. The cell apoptosis rate in PEG was significantly higher than those in TRCG or NPCG(P<0.01).
  
  
  CONCLUSIONS
  Cell growth was inhibited by shRNA against C-erbB2 gene. Cell cycle was blocked in G0/G1 phase and apoptosis was induced by C-erbB2 shRNA. This indicates C-erbB2 gene plays important roles in the carcinogenesis and development of colon cancer.
authors.affiliation: Department of Oncology, First Affiliated Hospital, Medical College of Xi'an Jiaotong University, Xi'an 710061, China. zhaodongli68@163.com
authors.forename: Dong-Li
authors.initials: DL
authors.lastname: Zhao
authors.forename: Cheng-Xue
authors.initials: CX
authors.lastname: Dang
authors.forename: Yan-Xia
authors.initials: YX
authors.lastname: Sui
mesh_terms: D017209:Apoptosis / Q000235:genetics* / Q000502:physiology; D002453:Cell Cycle / Q000235:genetics* / Q000502:physiology; D059447:Cell Cycle Checkpoints / Q000235:genetics / Q000502:physiology; D049109:Cell Proliferation; D002470:Cell Survival / Q000235:genetics / Q000502:physiology; D003110:Colonic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D005434:Flow Cytometry; D019073:HT29 Cells; D006801:Humans; D010957:Plasmids / Q000235:genetics; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics* / Q000378:metabolism; D013997:Time Factors; D014162:Transfection
chemical_list: D034741:RNA, Small Interfering; D018719:Erb-b2 Receptor Tyrosine Kinases; C508053:ERBB2 protein, human