title: Sortase-Mediated Transpeptidation for Site-Specific Modification of Peptides, Glycopeptides, and Proteins.
abstract: Sortases are a family of transpeptidases found in Gram-positive bacteria responsible for covalent anchoring of cell surface proteins to bacterial cell walls. It has been discovered that sortase A (SrtA) of Staphylococcus aureus origin is rather promiscuous and can accept various molecules as substrates. As a result, SrtA has been widely used to ligate peptides and proteins with a variety of nucleophiles, and the ligation products are useful for research in chemical biology, proteomics, biomedicine, etc. This review summarizes the recent applications of SrtA with special emphasis on SrtA-catalyzed ligation of carbohydrates with peptides and proteins.
authors.affiliation: Department of Chemistry, Wayne State University, 5101 Cass Avenue, Detroit 48202, USA.
authors.forename: Zhimeng
authors.initials: Z
authors.lastname: Wu
authors.forename: Zhongwu
authors.initials: Z
authors.lastname: Guo