title: Genome-wide analysis of cell wall-related genes in Tuber melanosporum.
abstract: A genome-wide inventory of proteins involved in cell wall synthesis and remodeling has been obtained by taking advantage of the recently released genome sequence of the ectomycorrhizal Tuber melanosporum black truffle. Genes that encode cell wall biosynthetic enzymes, enzymes involved in cell wall polysaccharide synthesis or modification, GPI-anchored proteins and other cell wall proteins were identified in the black truffle genome. As a second step, array data were validated and the symbiotic stage was chosen as the main focus. Quantitative RT-PCR experiments were performed on 29 selected genes to verify their expression during ectomycorrhizal formation. The results confirmed the array data, and this suggests that cell wall-related genes are required for morphogenetic transition from mycelium growth to the ectomycorrhizal branched hyphae. Labeling experiments were also performed on T. melanosporum mycelium and ectomycorrhizae to localize cell wall components.
authors.affiliation: Istituto per la Protezione delle Piante del CNR, UOS Torino, Viale Mattioli, 25, 10125, Turin, Italy. r.balestrini@ipp.cnr.it
authors.forename: Raffaella
authors.initials: R
authors.lastname: Balestrini
authors.forename: Fabiano
authors.initials: F
authors.lastname: Sillo
authors.forename: Annegret
authors.initials: A
authors.lastname: Kohler
authors.forename: Georg
authors.initials: G
authors.lastname: Schneider
authors.forename: Antonella
authors.initials: A
authors.lastname: Faccio
authors.forename: Emilie
authors.initials: E
authors.lastname: Tisserant
authors.forename: Francis
authors.initials: F
authors.lastname: Martin
authors.forename: Paola
authors.initials: P
authors.lastname: Bonfante
mesh_terms: D001203:Ascomycota / Q000145:classification / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D002473:Cell Wall / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D016681:Genome, Fungal; D023281:Genomics; D005936:Glucans / Q000378:metabolism; D010802:Phylogeny; D015203:Reproducibility of Results
chemical_list: D005656:Fungal Proteins; D005936:Glucans