title: A microplate technique to simultaneously assay calcium accumulation in endoplasmic reticulum and SERCA release of inorganic phosphate.
abstract: Traditional analyses of calcium homeostasis have separately quantified either calcium accumulation or release mechanisms. To define the system as a whole, however, requires multiple experimental techniques to examine both accumulation and release. Here we describe a technique that couples the simultaneous quantification of radio-labeled calcium accumulation in endoplasmic reticulum (ER) microsomes with the release of inorganic phosphate (Pi) by the hydrolytic activity of sarco-endoplasmic reticulum calcium ATPase (SERCA) all in the convenience of a 96-well format.
authors.affiliation: Department of Neurology, Uniformed Services University of the Health Sciences, B-3059, 4301 Jones Bridge Road, Bethesda, MD 20814, USA. david.mcmullen.ctr@usuhs.mil.
authors.forename: David C
authors.initials: DC
authors.lastname: McMullen
authors.forename: William S
authors.initials: WS
authors.lastname: Kean
authors.forename: Ajay
authors.initials: A
authors.lastname: Verma
authors.forename: Jeffrey T
authors.initials: JT
authors.lastname: Cole
authors.forename: William D
authors.initials: WD
authors.lastname: Watson