title: Identification of four novel alleles of the BoLA-DRB3 upstream regulatory region in Chinese yellow cattle.
abstract: The sequence of upstream regulatory region (URR) of BoLA-DRB3 gene was amplified with polymerase chain reaction followed by DNA sequencing from six animals of Chinese yellow cattle. A total of five alleles including four newly identified ones, named BoLA-DRB3*R-03-U2, BoLA-DRB3*R-06-U2, BoLA-DRB3*R-07-U and BoLA-DRB3*R-12-U for the BoLA-DRB3 URR were found. Result of sequence analysis showed that the regulatory elements W, X, Y, CCAAT and TATA-like boxes existed in such URRs and 16 polymorphic sites (11 transitions, 3 transversions, 1 deletion and 1 insertion) located in the spacers between the conserved consensus boxes and 1 insertion within X box, while no new polymorphic site within the consensus boxes.
authors.affiliation: Institute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang, China. kunwl@126.com
authors.forename: K
authors.initials: K
authors.lastname: Wang
authors.forename: D-X
authors.initials: DX
authors.lastname: Sun
authors.forename: K-Y
authors.initials: KY
authors.lastname: Li
authors.forename: X-Q
authors.initials: XQ
authors.lastname: Wang
authors.forename: F
authors.initials: F
authors.lastname: Zhang
mesh_terms: D000483:Alleles; D000818:Animals; D002417:Cattle / Q000276:immunology; D002681:China; D003001:Cloning, Molecular; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D006650:Histocompatibility Testing; D009154:Mutation / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D016415:Sequence Alignment
chemical_list: C423282:BoLA-DRB3 antigen; D000949:Histocompatibility Antigens Class II