title: Highly sensitive detection of alkaline phosphatase using molecular beacon probes based on enzymatic polymerization.
abstract: We have developed a new methodology for highly sensitive alkaline phosphatase assay using molecular beacon probes. No incubation step is needed to obtain a limit of detection for ALP of 2×10(-16) M. Furthermore, ALP inhibition by the inhibitor okadaic acid is shown, demonstrating the potential for high-throughput screening for inhibitors.
authors.affiliation: School of Biological Science and Technology, Central South University, Changsha 410013, China. lhlmcb@yahoo.com.cn
authors.forename: Changbei
authors.initials: C
authors.lastname: Ma
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis* / Q000037:antagonists & inhibitors; D015335:Molecular Probes / Q000737:chemistry*; D019319:Okadaic Acid / Q000737:chemistry; D058105:Polymerization
chemical_list: D015335:Molecular Probes; D019319:Okadaic Acid; D000469:Alkaline Phosphatase