title: Maintenance of surface antigens and the absence of an apoptotic marker are observed during storage of granulocyte concentrates collected by bag separation method.
abstract: Granulocytes were collected by the bag separation method and stored in whole blood for up to 72h. We evaluated the expressions of various surface antigens: CD62L, CD11b, CD18, CD64, CD16b, and CD95. Apoptosis was assessed both by flow cytometry and by light microscopy. Expression levels of all the surface antigens were shown to be maintained during storage for up to 72h. Approximately 80% of granulocytes were annexin V negative until 72h after collection. The storage of granulocyte concentrates collected by the bag separation method may maintain granulocyte surface antigens and lack an apoptotic marker.
authors.affiliation: Department of Pediatrics, Fukushima Medical University School of Medicine, Fukushima, Japan. m-akai@fmu.ac.jp
authors.forename: M
authors.initials: M
authors.lastname: Akaihata
authors.forename: A
authors.initials: A
authors.lastname: Kikuta
authors.forename: K
authors.initials: K
authors.lastname: Mochizuki
authors.forename: K
authors.initials: K
authors.lastname: Nemoto
authors.forename: M
authors.initials: M
authors.lastname: Ito
authors.forename: H
authors.initials: H
authors.lastname: Sano
authors.forename: S
authors.initials: S
authors.lastname: Kobayashi
authors.forename: H
authors.initials: H
authors.lastname: Ohto
authors.forename: M
authors.initials: M
authors.lastname: Hosoya
mesh_terms: D015703:Antigens, CD / Q000378:metabolism*; D017209:Apoptosis; D015415:Biomarkers / Q000378:metabolism; D001793:Blood Preservation / Q000295:instrumentation* / Q000379:methods*; D005260:Female; D006098:Granulocytes / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D008297:Male; D013997:Time Factors
chemical_list: D015703:Antigens, CD; D015415:Biomarkers