title: Non-destructive detection of adulterated tablets of glibenclamide using NIR and solid-phase fluorescence spectroscopy and chemometric methods.
abstract: This study describes a method for non-destructive detection of adulterated glibenclamide tablets. This method uses near infrared spectroscopy (NIRS) and fluorescence spectroscopy along with chemometric tools such as Soft Independent Modeling of Class Analogy (SIMCA), Partial Least Squares-Discriminant Analysis (PLS-DA) and Unfolded Partial Least Squares with Discriminant Analysis (UPLS-DA). Both brand name (Daonil) and generic glibenclamide tablets were used for analysis. The levels of glibenclamide in each type of tablet were evaluated by derivative spectrophotometry in the ultraviolet region. The results obtained from the NIR and fluorescence spectroscopy along with those obtained from multivariate data classification show that this combined technique is an effective way to detect adulteration in drugs for the treatment of diabetes. In the future, this method may be extended to detect different types of counterfeit medications.
authors.affiliation: Universidade Federal do Rio Grande do Norte, Instituto de Química, Grupo de Pesquisa em Quimiometria Aplicada, CEP 59072-970, Natal, RN, Brazil.
authors.forename: Rafael
authors.initials: R
authors.lastname: da Silva Fernandes
authors.forename: Fernanda Saadna Lopes
authors.initials: FS
authors.lastname: da Costa
authors.forename: Patrícia
authors.initials: P
authors.lastname: Valderrama
authors.forename: Paulo Henrique
authors.initials: PH
authors.lastname: Março
authors.forename: Kássio Michell Gomes
authors.initials: KM
authors.lastname: de Lima
mesh_terms: D058110:Counterfeit Drugs / Q000032:analysis / Q000737:chemistry; D016002:Discriminant Analysis; D004340:Drug Contamination; D005905:Glyburide / Q000032:analysis* / Q000737:chemistry / Q000592:standards; D007004:Hypoglycemic Agents / Q000032:analysis* / Q000737:chemistry / Q000592:standards; D016018:Least-Squares Analysis; D008956:Models, Chemical; D015999:Multivariate Analysis; D013050:Spectrometry, Fluorescence / Q000379:methods*; D019265:Spectroscopy, Near-Infrared / Q000379:methods*; D013607:Tablets
chemical_list: D058110:Counterfeit Drugs; D007004:Hypoglycemic Agents; D013607:Tablets; D005905:Glyburide