title: Nucleotide sequencing and serologic analysis of Cache Valley virus isolates from the Yucatan Peninsula of Mexico.
abstract: Nucleotide sequencing was performed on part of the medium and large genome segments of 17 Cache Valley virus (CVV) isolates from the Yucatan Peninsula of Mexico. Alignment of these sequences to all other sequences in the Genbank database revealed that they have greatest nucleotide identity (97-98 %) with the equivalent regions of Tlacotalpan virus (TLAV), which is considered to be a variety of CVV. Next, cross-plaque reduction neutralization tests (PRNTs) were performed using sera from mice that had been inoculated with a representative isolate from the Yucatan Peninsula (CVV-478) or the prototype TLAV isolate (61-D-240). The PRNT titers exhibited a twofold difference in one direction and no difference in the other direction suggesting that CVV-478 and 61-D-240 belong to the same CVV subtype. In conclusion, we demonstrate that the CVV isolates from the Yucatan Peninsula of Mexico are genetically and antigenically similar to the prototype TLAV isolate.
authors.affiliation: Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA. blitvich@iastate.edu
authors.forename: Bradley J
authors.initials: BJ
authors.lastname: Blitvich
authors.forename: Maria A
authors.initials: MA
authors.lastname: Loroño-Pino
authors.forename: Julian E
authors.initials: JE
authors.lastname: Garcia-Rejon
authors.forename: Jose A
authors.initials: JA
authors.lastname: Farfan-Ale
authors.forename: Karin S
authors.initials: KS
authors.lastname: Dorman
mesh_terms: D000330:Aedes / Q000821:virology*; D000818:Animals; D002042:Bunyamwera virus / Q000145:classification / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D005260:Female; D007106:Immune Sera / Q000276:immunology; D008800:Mexico; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D010948:Viral Plaque Assay
chemical_list: D007106:Immune Sera