title: Protection against pneumococcal infection elicited by immunization with glutamyl tRNA synthetase, polyamine transport protein D and sortase A.
abstract: Protein-based vaccines are considered to be the next-generation of pneumococcal vaccines. Here we evaluated the protection elicited by immunization with recombinant glutamyl tRNA synthetase (Gts), polyamine transport protein D (PotD) and sortase A (SrtA) antigens in preclinical mouse models. In mucosal immunization studies, intranasal immunization with either Gts, PotD or SrtA could significantly reduce pneumococcal nasopharyngeal and lung colonization and significantly increase mice survival times following invasive pneumococcal challenge, and combinations of these antigens could enhance this protection. In systemic immunization studies, intraperitoneal immunization with multiple protein antigens also provided better protection against pneumococcal sepsis caused by different pneumococcal strains. Finally, passive immunization studies showed an additive effect by using multiple anti-sera when compared to single anti-sera. Therefore, a multicomponent protein-based pneumococcal vaccine composed of Gts, PotD or SrtA could confer protection against pneumococcal colonization as well as invasive infections in terms of efficacy of protection and serotype coverage.
authors.affiliation: Department of Laboratory Medicine, Chongqing Medical University, Chongqing, China.
authors.forename: Xun
authors.initials: X
authors.lastname: Min
authors.forename: Xuemei
authors.initials: X
authors.lastname: Zhang
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Yi
authors.initials: Y
authors.lastname: Gong
authors.forename: Meijuan
authors.initials: M
authors.lastname: Li
authors.forename: Wenchun
authors.initials: W
authors.lastname: Xu
authors.forename: Yibing
authors.initials: Y
authors.lastname: Yin
authors.forename: Ju
authors.initials: J
authors.lastname: Cao
mesh_terms: D000281:Administration, Intranasal; D019881:Aminoacyltransferases / Q000008:administration & dosage / Q000276:immunology*; D000818:Animals; D001426:Bacterial Proteins / Q000008:administration & dosage / Q000276:immunology*; D003546:Cysteine Endopeptidases / Q000008:administration & dosage / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D005975:Glutamate-tRNA Ligase / Q000008:administration & dosage / Q000276:immunology*; D007114:Immunization / Q000379:methods*; D008168:Lung / Q000382:microbiology; D026901:Membrane Transport Proteins / Q000008:administration & dosage / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009305:Nasopharynx / Q000382:microbiology; D011008:Pneumococcal Infections / Q000276:immunology / Q000517:prevention & control*; D022242:Pneumococcal Vaccines / Q000008:administration & dosage / Q000276:immunology*; D016019:Survival Analysis; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology
chemical_list: D001426:Bacterial Proteins; D026901:Membrane Transport Proteins; D022242:Pneumococcal Vaccines; D022223:Vaccines, Subunit; D014614:Vaccines, Synthetic; D019881:Aminoacyltransferases; C120542:sortase A; D003546:Cysteine Endopeptidases; D005975:Glutamate-tRNA Ligase