title: Fluorescence imaging of potassium ions in living cells using a fluorescent probe based on a thrombin binding aptamer-peptide conjugate.
abstract: When a biotinylated FRET probe based on a peptide-thrombin binding aptamer conjugate was introduced together with streptavidin and biotinylated nuclear export signal peptide into HeLa cells, the resulting ternary complex enabled visualization of K(+) concentration changes in the cell.
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Kyushu Institute of Technology, Fukuoka 804-8550, Japan.
authors.forename: Keiichi
authors.initials: K
authors.lastname: Ohtsuka
authors.forename: Shinobu
authors.initials: S
authors.lastname: Sato
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Sato
authors.forename: Kojiro
authors.initials: K
authors.lastname: Sota
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Ohzawa
authors.forename: Tomoki
authors.initials: T
authors.lastname: Matsuda
authors.forename: Kiwamu
authors.initials: K
authors.lastname: Takemoto
authors.forename: Nobutoki
authors.initials: N
authors.lastname: Takamune
authors.forename: Bernard
authors.initials: B
authors.lastname: Juskowiak
authors.forename: Takeharu
authors.initials: T
authors.lastname: Nagai
authors.forename: Shigeori
authors.initials: S
authors.lastname: Takenaka
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D007477:Ions; D010455:Peptides / Q000737:chemistry*; D011188:Potassium / Q000032:analysis / Q000737:chemistry*; D019809:Streptavidin / Q000737:chemistry
chemical_list: D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; D007477:Ions; D010455:Peptides; C081676:thrombin aptamer; D019809:Streptavidin; D011188:Potassium