title: Metabolites of Lactobacillus plantarum 2142 prevent oxidative stress-induced overexpression of proinflammatory cytokines in IPEC-J2 cell line.
abstract: Probiotics have already proven beneficial effects in the treatment of several intestinal infections, but the underlying mechanisms how the probiotics can affect responses of porcine IPEC-J2 enterocytes to oxidative stress remained to be elucidated. The immunomodulatory effect of five bacterial strains (Lactobacillus plantarum 2142, Lactobacillus casei Shirota, Bifidobacterium animalis subsp. lactis BB-12, Bacillus amyloliquefaciens CECT 5940 and Enterococcus faecium CECT 4515) on 1 mM peroxide-triggered upregulation of interleukin (IL)-8 and tumor necrosis factor alpha (TNF-α) level was screened by q RT-PCR. Our data revealed that spent culture supernatant (SCS) of L. plantarum 2142 had significant lowering effect on IL-8 and TNF-α level with concomitant promoting activity on protective Hsp70 gene expression. According to our results, lactic acid (racemic, D: - and L: -lactic acid) and acetic acid produced by lactobacilli had no protective effect in quenching upregulation of proinflammatory cytokines. Furthermore, L. plantarum 2142-specific supernatant peptides were detected by gel electrophoresis and capillary zone electrophoresis.
authors.affiliation: Department of Pharmacology and Toxicology, Faculty of Veterinary Sciences, Szent István University, István u. 2, 1078, Budapest, Hungary. Gere.Erzsebet@aotk.szie.hu
authors.forename: Erzsebet
authors.initials: E
authors.lastname: Paszti-Gere
authors.forename: Krisztina
authors.initials: K
authors.lastname: Szeker
authors.forename: Edina
authors.initials: E
authors.lastname: Csibrik-Nemeth
authors.forename: Rita
authors.initials: R
authors.lastname: Csizinszky
authors.forename: Andras
authors.initials: A
authors.lastname: Marosi
authors.forename: Orsolya
authors.initials: O
authors.lastname: Palocz
authors.forename: Orsolya
authors.initials: O
authors.lastname: Farkas
authors.forename: Peter
authors.initials: P
authors.lastname: Galfi
mesh_terms: D019342:Acetic Acid / Q000378:metabolism; D000818:Animals; D001407:Bacillus / Q000378:metabolism; D001422:Bacterial Adhesion; D001644:Bifidobacterium / Q000378:metabolism; D002460:Cell Line; D017097:Electric Impedance; D016984:Enterococcus faecium / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis; D016209:Interleukin-8 / Q000096:biosynthesis* / Q000235:genetics; D007413:Intestinal Mucosa / Q000382:microbiology; D019344:Lactic Acid / Q000096:biosynthesis / Q000378:metabolism; D007780:Lacticaseibacillus casei / Q000378:metabolism; D048191:Lactiplantibacillus plantarum / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*; D019936:Probiotics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species; D013552:Swine; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis* / Q000235:genetics; D015854:Up-Regulation
chemical_list: D018840:HSP70 Heat-Shock Proteins; D016209:Interleukin-8; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; D019344:Lactic Acid; D019342:Acetic Acid