title: H-2 antigen variants in a cultured heterozygous mouse leukemia cell line. VII. Effect of selection with a hybridoma antibody.
abstract: An (H-2k/H-2d)F1 sarcoma cell line was subjected to immunoselection using ascites fluid from a mouse growing a hybridoma secreting an anti H-2Kk antibody.One hundred random clones were picked from the surviving population and screened by direct cytolysis using the hybridoma antibody or alloantisera against H-2Kk and H-2Dk. Fifty-nine clones were resistant to all three antisera, indicating that they no longer expressed the entire H-2k haplotype. Thirty-two were resistant to the ascites and to the anti H-2Kk alloantiserum, but sensitive to the anti H-2Dk serum, indicating that they had lost H-2Kk antigen, but retained H-2Dk. Nine clones were sensitive to the alloantisera, but resistant to the hybridoma, indicating that, though they retained the product(s) recognized by the alloantiserum against H-2Kk, they had lost the site(s) that bound the hybridoma antibody. Quantitative absorption assays using lymph-node cells from young BALB.K (H-2Kk) mice as targets show that one representative clone from the last group absorbs the anti H-2Kk activity in the alloantiserum. This implies that the sensitivity of the variant clone to the alloantiserum is not due to contaminating anti C-type virus antibodies in the serum. The possible implications of these data are discussed.
authors.affiliation: Department of Pathology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
authors.forename: T V
authors.initials: TV
authors.lastname: Rajan
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D045744:Cell Line, Tumor; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D006183:H-2 Antigens / Q000276:immunology*; D006239:Haplotypes / Q000276:immunology; D006579:Heterozygote; D064532:Histocompatibility Antigen H-2D; D006825:Hybridomas / Q000166:cytology / Q000276:immunology*; D007106:Immune Sera / Q000276:immunology; D007518:Isoantibodies / Q000276:immunology*; D007938:Leukemia / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D016296:Mutagenesis; D012509:Sarcoma / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D006183:H-2 Antigens; C024755:H-2K(K) antigen; D064532:Histocompatibility Antigen H-2D; D007106:Immune Sera; D007518:Isoantibodies; D005020:Ethyl Methanesulfonate