title: On-line two dimensional liquid chromatography/mass spectrometry for the analysis of triacylglycerides in peanut oil and mouse tissue.
abstract: Triacylglycerides (TAGs) are a large class of complex neutral lipids that naturally occur in both plants and animals. In the present work, an on-line comprehensive silver-ion liquid chromatography (silver-ion LC) × reversed-phase liquid chromatography (RPLC) system was constructed to analyze these compounds. A micro bore silver-ion modified column was employed in the first dimension with the commonly used hexane-based mobile phase. After a series of C18 columns were assessed, a wide bore column packed with 1.5 μm particles was selected as the second dimension column to reduce the negative effect caused by the large volume and strong solvent injection in the second dimension. The system coupled with mass spectrometry was applied to the analysis of an edible peanut oil and a mouse liver extract. Twenty-eight TAGs from the peanut oil and forty-four from the mouse liver were identified based on the TAGs' retention behaviors on the comprehensive two-dimensional LC system and their APCI MS fragments.
authors.affiliation: CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Yang
authors.forename: Xianzhe
authors.initials: X
authors.lastname: Shi
authors.forename: Qun
authors.initials: Q
authors.lastname: Gu
authors.forename: Sumin
authors.initials: S
authors.lastname: Zhao
authors.forename: Yuanhong
authors.initials: Y
authors.lastname: Shan
authors.forename: Guowang
authors.initials: G
authors.lastname: Xu
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D008099:Liver / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D051379:Mice; D000074241:Peanut Oil; D010938:Plant Oils / Q000737:chemistry*; D012834:Silver / Q000737:chemistry; D014280:Triglycerides / Q000032:analysis* / Q000737:chemistry / Q000145:classification
chemical_list: D000074241:Peanut Oil; D010938:Plant Oils; D014280:Triglycerides; D012834:Silver