title: Enhancing effects of trichloroethylene and tetrachloroethylene on type I allergic responses in mice.
abstract: Trichloroethylene (TCE) and tetrachloroethylene (perchloroethylene; PCE) are commonly identified as environmental contaminants of groundwater. Previously, we investigated the enhancing effects of TCE and PCE on antigen-induced histamine release and inflammatory mediator production in rat mast cells. In this study, to examine the potential effect of TCE and PCE on antigen-induced histamine release from mouse mast cells, mouse bone marrow-derived mast cells (BMMC) were sensitized with anti-dinitrophenol (DNP) monoclonal IgE antibody and then stimulated with DNP-BSA containing with TCE or PCE. Both TCE and PCE significantly enhanced antigen-induced histamine release from BMMC. Next we investigated the effects of TCE and PCE on the passive cutaneous anaphylaxis (PCA) reaction in vivo using ICR mice. TCE and PCE significantly enhanced the PCA reaction in a dose-dependent manner. In addition, we examined the enhancing effects of ingesting small amount of TCE and PCE in drinking water on antigen-stimulated allergic responses. After the ICR mice had ingested TCE or PCE in their drinking water for 2 or 4 weeks, we performed the PCA reaction. Both TCE and PCE ingestion enhanced the PCA reaction in a dose-dependent manner for 4 weeks. These results suggest that exposure to TCE and PCE leads to the augmentation of type I allergic responses in many species.
authors.forename: Makoto
authors.initials: M
authors.lastname: Seo
authors.forename: Ryo
authors.initials: R
authors.lastname: Kobayashi
authors.forename: Tetsunori
authors.initials: T
authors.lastname: Okamura
authors.forename: Koji
authors.initials: K
authors.lastname: Ikeda
authors.forename: Masahiko
authors.initials: M
authors.lastname: Satoh
authors.forename: Naoki
authors.initials: N
authors.lastname: Inagaki
authors.forename: Hiroichi
authors.initials: H
authors.lastname: Nagai
authors.forename: Hisamitsu
authors.initials: H
authors.lastname: Nagase
mesh_terms: D000818:Animals; D004140:Dinitrophenols / Q000276:immunology; D004342:Drug Hypersensitivity / Q000276:immunology*; D006632:Histamine / Q000276:immunology; D007155:Immunologic Factors / Q000633:toxicity*; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects*; D012710:Serum Albumin, Bovine / Q000276:immunology; D012997:Solvents / Q000633:toxicity; D013750:Tetrachloroethylene / Q000633:toxicity*; D014241:Trichloroethylene / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity
chemical_list: D004140:Dinitrophenols; D007155:Immunologic Factors; D012997:Solvents; D014874:Water Pollutants, Chemical; D012710:Serum Albumin, Bovine; D014241:Trichloroethylene; D006632:Histamine; D013750:Tetrachloroethylene