title: Overexpression of DNA polymerase zeta reduces the mitochondrial mutability caused by pathological mutations in DNA polymerase gamma in yeast.
abstract: In yeast, DNA polymerase zeta (Rev3 and Rev7) and Rev1, involved in the error-prone translesion synthesis during replication of nuclear DNA, localize also in mitochondria. We show that overexpression of Rev3 reduced the mtDNA extended mutability caused by a subclass of pathological mutations in Mip1, the yeast mitochondrial DNA polymerase orthologous to human Pol gamma. This beneficial effect was synergistic with the effect achieved by increasing the dNTPs pools. Since overexpression of Rev3 is detrimental for nuclear DNA mutability, we constructed a mutant Rev3 isoform unable to migrate into the nucleus: its overexpression reduced mtDNA mutability without increasing the nuclear one.
authors.affiliation: Department of Genetics, Biology of Microorganisms, Anthropology, Evolution, University of Parma, Parma, Italy. enrico.baruffini@nemo.unipr.it
authors.forename: Enrico
authors.initials: E
authors.lastname: Baruffini
authors.forename: Fausta
authors.initials: F
authors.lastname: Serafini
authors.forename: Iliana
authors.initials: I
authors.lastname: Ferrero
authors.forename: Tiziana
authors.initials: T
authors.lastname: Lodi
mesh_terms: D004256:DNA Polymerase I / Q000235:genetics / Q000378:metabolism*; D000074002:DNA Polymerase gamma; D004272:DNA, Mitochondrial / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D008928:Mitochondria / Q000201:enzymology*; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D004272:DNA, Mitochondrial; D029701:Saccharomyces cerevisiae Proteins; C425929:DNA polymerase zeta; D004256:DNA Polymerase I; D000074002:DNA Polymerase gamma; D004259:DNA-Directed DNA Polymerase; C068352:MIP1 protein, S cerevisiae