title: [Screening  for   genes associated with cardiac fibrosis induced by aldosterone].
abstract: OBJECTIVE
  To investigate differently expressed genes associated with cardiac fibrosis induced independently by aldosterone.
  
  
  METHODS
  Fetal cardiac fibroblasts (FCFs)were isolated and cultured. Total RNA was extracted 8 hours after aldosterone administration. Then gene chips were used to screen these RNA samples. Some of candidate genes were confirmed by RT-PCR and Western blot.
  
  
  RESULTS
  Differently expressed 1519 genes were screened. Up-regulated genes were 714 while down-regulated  genes were 805. The expression of CCL7,  MMP-26 and IL31RA was tested by RT-PCR and western blot,  the results is identical with those by gene chips.
  
  
  CONCLUSIONS
  Gene chip can efficiently single out differently expressed genes induced dependently by aldosterone in FCFs. CCL7,  MMP-26 and IL31RA may be associated with cardiac fibrosis induced by aldosterone.
authors.affiliation: Department of Cardiology, PLA General Hospital, Beijing 100853, China. cjsong001@sina.com
authors.forename: Jin-Song
authors.initials: JS
authors.lastname: Chen
authors.forename: Qun
authors.initials: Q
authors.lastname: Mo
authors.forename: Yong-Jin
authors.initials: YJ
authors.lastname: Xie
authors.forename: Yu-Song
authors.initials: YS
authors.lastname: Guo
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Huang
authors.forename: Lei
authors.initials: L
authors.lastname: Ren
authors.forename: Sheng-Qi
authors.initials: SQ
authors.lastname: Wang
authors.forename: Xiao-Chen
authors.initials: XC
authors.lastname: Bo
authors.forename: Yi-Hong
authors.initials: YH
authors.lastname: Ren
mesh_terms: D000450:Aldosterone / Q000494:pharmacology*; D015153:Blotting, Western; D002478:Cells, Cultured; D054410:Chemokine CCL7 / Q000235:genetics / Q000378:metabolism; D005333:Fetus; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D005355:Fibrosis / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects*; D020869:Gene Expression Profiling; D006801:Humans; D053505:Matrix Metalloproteinases, Secreted / Q000235:genetics / Q000378:metabolism; D009206:Myocardium; D020411:Oligonucleotide Array Sequence Analysis; D018123:Receptors, Interleukin / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C515450:CCL7 protein, human; D054410:Chemokine CCL7; C502188:IL31RA protein, human; D018123:Receptors, Interleukin; D000450:Aldosterone; C410599:MMP26 protein, human; D053505:Matrix Metalloproteinases, Secreted