title: β-Glucosidase involvement in the formation and transformation of oleuropein during the growth and development of olive fruits (Olea europaea L. cv. Arbequina) grown under different farming practices.
abstract: The present study investigates oleuropein metabolism, as well as the involvement of β-glucosidase during the growth, development, and ripening of olive fruit. The results show that in olive fruit the in vivo formation and transformation of oleuropein takes place in three different stages. The first one is characterized by a net accumulation of oleuropein and occurs in the immature fruit. In the second stage, associated with the green and light-green fruits, oleuropein content is maintained practically constant, and finally, a third stage that begins in the green-yellow fruit is characterized by a progressive decline of the oleuropein concentration. Our findings confirm that in the absence of β-glucosidase the Damtoft-proposed pathway is active and that net synthesis of oleuropein is unquestionable. β-Glucosidase activity plays a key role in the oleuropein metabolism catalyzing its in vivo hydrolysis.
authors.affiliation: Grupo de Química y Bioquímica de Pigmentos, Departamento de Biotecnología de Alimentos, Instituto de la Grasa-CSIC, Avenida Padre García Tejero 4, 41012 Seville, Spain. rosales@cica.es
authors.forename: Francisca
authors.initials: F
authors.lastname: Gutierrez-Rosales
authors.forename: María Paz
authors.initials: MP
authors.lastname: Romero
authors.forename: María
authors.initials: M
authors.lastname: Casanovas
authors.forename: María José
authors.initials: MJ
authors.lastname: Motilva
authors.forename: María Isabel
authors.initials: MI
authors.lastname: Mínguez-Mosquera
mesh_terms: D000383:Agriculture / Q000379:methods*; D005638:Fruit / Q000254:growth & development*; D057888:Iridoid Glucosides; D039823:Iridoids; D031658:Olea; D058871:Organic Agriculture / Q000379:methods*; D011714:Pyrans / Q000378:metabolism*; D012621:Seasons; D001617:beta-Glucosidase / Q000378:metabolism*
chemical_list: D057888:Iridoid Glucosides; D039823:Iridoids; D011714:Pyrans; C002769:oleuropein; D001617:beta-Glucosidase