title: The development of a novel percutaneous lung biopsy procedure for use on feedlot steers.
abstract: The purpose of this study was to develop a percutaneous lung biopsy technique to be used on steers in a commercial feedlot setting. Thirty-four crossbred steer and heifer calves from a commercial feedlot in southern Alberta were used in this study. The calves originated from the auction market and all were chronically affected with bovine respiratory disease (BRD). A technique was developed to obtain a lung sample from the right cranioventral lung lobe, intercostal space (ICS) 2, using a manual or an automatic biopsy instrument with a 14- or 12-gauge (ga) biopsy needle. Overall, lung parenchyma was successfully harvested in 55.9% of experimental animals and in 55.0% of lung biopsy trials. Compared with postmortem diagnosis, the biopsy resulted in the same pathologic diagnosis for 75% of biopsy samples when evaluated using standardized criteria by the same veterinary pathologist. The success rate was 61.5% and 42.9% in a hospital or field setting, respectively. With an automatic instrument, lung was recovered from 57.9% and 37.5% of samples obtained using a 12- or 14-ga biopsy needle, respectively. One experimental animal or 2.9% of the total had fatal complications from the procedure. In a commercial feedlot setting, the procedure took 20 min for each animal. Percutaneous lung biopsy of the right cranioventral lung lobe may be a viable technique when used on feedlot steers affected with chronic pneumonia. These findings suggest that using an automatic instrument with either a 14- or 12-ga biopsy needle may yield lung samples that are suitable for histopathological evaluation. However, this technique needs to be further evaluated in a field setting.
authors.affiliation: Department of Large Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan. brandy.burgess@colostate.edu
authors.forename: Brandy A
authors.initials: BA
authors.lastname: Burgess
authors.forename: Steve H
authors.initials: SH
authors.lastname: Hendrick
authors.forename: Colleen M
authors.initials: CM
authors.lastname: Pollock
authors.forename: Sameeh M
authors.initials: SM
authors.lastname: Abutarbush
authors.forename: Amanda
authors.initials: A
authors.lastname: Vogstad
authors.forename: G Kee
authors.initials: GK
authors.lastname: Jim
authors.forename: Calvin W
authors.initials: CW
authors.lastname: Booker
mesh_terms: D000818:Animals; D001707:Biopsy, Needle / Q000379:methods / Q000662:veterinary; D002417:Cattle; D002418:Cattle Diseases / Q000473:pathology*; D005260:Female; D008171:Lung Diseases / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary*; D008297:Male