title: Posttraumatic temporal TGF-β mRNA expression in lens epithelial cells of paediatric patients.
abstract: The aim of the study was to determine temporal           TGFB1, TGFB2 and TGFB3 gene expression           profiles in the anterior lens capsule of paediatric patients           with posttraumatic cataract. The patient group           comprised 22 children selected with a fragment of           anterior lens capsule obtained during elective cataract           surgery and sampled for molecular analysis.           The levels of TGF-β isoforms in the anterior lens           capsule were determined based on the number of           mRNA copies per 1 μg total RNA by real-time qRTPCR.           Three time-related result clusters were identified           based on hierarchical cluster analysis: 2.2, 4.4           and 15.0 months (time span from injury to anterior           capsule sampling during surgery) and compared           with regard to temporal gene expression profile and           quantitative relations of TGF-β1, 2 and 3 mRNAs.           TGF-β1, TGF-β2, and TGF-β3 mRNAs were detected           in all anterior lens capsule samples. A comparative           analysis revealed: TGF-β1>TGF-β2>TGF-β3           during the entire observation period. The TGF-β           mRNA levels continued to increase up to four months           after injury, then returning close to the base levels           after around 15 months. The expression patterns of           TGF-β isoforms showed a similar tendency. Differences           in the expression levels of TGF-β1 and TGF-β2           between the particular clusters were statistically significant.            Posttraumatic transcriptional activities of           TGF-β1 and TGF-β2 in the anterior lens capsule of           paediatric patients depend on the time elapsing from           injury. Our findings indicate that the transcriptional           activities of TGFB family genes show a transient period           of over-expression during the months after injury.           TGF-β1 is a dominant isoform expressed in lens           epithelial cells following injury.
authors.affiliation: Division of Pediatric Ophthalmology, Department of Ophthalmology, Medical University of Silesia, Katowice, Poland. berezbba@gmail.com
authors.forename: P
authors.initials: P
authors.lastname: Berezowski
authors.forename: B
authors.initials: B
authors.lastname: Strzalka-Mrozik
authors.forename: M
authors.initials: M
authors.lastname: Forminska-Kapuscik
authors.forename: U
authors.initials: U
authors.lastname: Mazurek
authors.forename: E
authors.initials: E
authors.lastname: Filipek
authors.forename: L
authors.initials: L
authors.lastname: Nawrocka
authors.forename: E
authors.initials: E
authors.lastname: Pieczara
authors.forename: P
authors.initials: P
authors.lastname: Banasiak
authors.forename: M
authors.initials: M
authors.lastname: Kimsa
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D004847:Epithelial Cells / Q000378:metabolism*; D015807:Eye Injuries, Penetrating / Q000235:genetics* / Q000473:pathology; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007903:Lens Capsule, Crystalline / Q000378:metabolism / Q000473:pathology; D007908:Lens, Crystalline / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000235:genetics* / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D016212:Transforming Growth Factor beta