title: cDNA microarray analysis of disk abalone genes in gills and hemocytes after viral hemorrhagic septicemia virus (VHSV) challenge.
abstract: A disk abalone Haliotis discus discus 4.2 K cDNA microarray was designed by selecting abalone expressed sequence tags (ESTs). Transcriptional profiles in gills and hemocytes were analyzed upon abalone challenged with viral hemorrhagic septicemia virus (VHSV) in order to select candidates for screening of immune response genes. Among the 4188 genes analyzed, 280 (6.6%) transcripts were changed their expression level in gills and hemocytes against VHSV challenge compared to control animals. Total of 88 and 65 genes were up-regulated in gills and hemocytes, respectively. These genes can be grouped under various immune-functional categories such as transcription factors (Krüppell-like factor; ETS-family transcription factor), inflammatory and apoptosis related genes (TNF superfamily members, Fas ligand), IFN regulatory proteins (IFN-44 like, interferon gamma-inducible lysosomal thiol reductase) and detoxification proteins (glutathione peroxidase). In contrast, 25 and 102 genes were shown down-regulation in gills and hemocytes, respectively. Among the differentially expressed transcripts, considerably higher numbers of ESTs were represented as either hypothetical (unknown) proteins or no GenBank match suggesting those may be novel genes associated with internal defense of abalone.
authors.affiliation: College of Veterinary Medicine, Chungnam National University, Yuseong-gu, Daejeon, Republic of Korea. mahanama@cnu.ac.kr
authors.forename: Mahanama
authors.initials: M
authors.lastname: De Zoysa
authors.forename: Chamilani
authors.initials: C
authors.lastname: Nikapitiya
authors.forename: Chulhong
authors.initials: C
authors.lastname: Oh
authors.forename: Ilson
authors.initials: I
authors.lastname: Whang
authors.forename: Hyun-Jin
authors.initials: HJ
authors.lastname: Shin
authors.forename: Jehee
authors.initials: J
authors.lastname: Lee
mesh_terms: D000818:Animals; D049851:Gastropoda / Q000235:genetics* / Q000821:virology*; D020869:Gene Expression Profiling; D005880:Gills / Q000378:metabolism / Q000821:virology; D006434:Hemocytes / Q000378:metabolism / Q000821:virology; D029141:Novirhabdovirus / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D060888:Real-Time Polymerase Chain Reaction