title: Association analysis of IL-17A and IL-17F polymorphisms in Chinese Han women with breast cancer.
abstract: BACKGROUND
  Research into the etiology of breast cancer has recently focused on the role of the immunity and inflammation. The proinflammatory cytokines IL-17A and IL-17F can mediate inflammation and cancer. To evaluate the influences of IL-17A and IL-17F gene polymorphisms on the risk of sporadic breast cancer, a case-control study was conducted in Chinese Han women.
  
  
  RESULTS
  We genotyped three single-nucleotide polymorphisms (SNPs) in IL-17A (rs2275913, rs3819025 and rs3748067) and five SNPs in IL-17F (rs7771511, rs9382084, rs12203582, rs1266828 and rs763780) to determine the haplotypes in 491 women with breast cancer and 502 healthy individuals. The genotypes were determined using the SNaPshot technique. The differences in the genotypic distribution between breast cancer patients and healthy controls were analyzed with the Chi-square test for trends. For rs2275913 in IL-17A, the frequency of the AA genotype was higher in patients than controls (P = 0.0016). The clinical features analysis demonstrated significant associations between IL-17 SNPs and tumor protein 53 (P53), progesterone receptor (PR), human epidermal growth factor receptor 2 (Her-2) and triple-negative (ER-/PR-/Her-2-) status. In addition, the haplotype analysis indicated that the frequency of the haplotype A(rs2275913)G(rs3819025)G(rs3748067), located in the IL-17A linkage disequilibrium (LD) block, was higher in patients than in controls (P = 0.0471 after correction for multiple testing).
  
  
  CONCLUSIONS
  Our results suggested that SNPs in IL-17A but not IL-17F were associated with the risk of breast cancer. Both IL-17A and IL-17F gene polymorphisms may provide valuable information for predicting the prognosis of breast cancer in Chinese women.
authors.affiliation: Institute of Cancer Prevention and Treatment, Harbin Medical University, Harbin, China.
authors.forename: Lihong
authors.initials: L
authors.lastname: Wang
authors.forename: Yongdong
authors.initials: Y
authors.lastname: Jiang
authors.forename: Youxue
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yuwen
authors.initials: Y
authors.lastname: Wang
authors.forename: Sunhui
authors.initials: S
authors.lastname: Huang
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Wang
authors.forename: Baoling
authors.initials: B
authors.lastname: Tian
authors.forename: Yue
authors.initials: Y
authors.lastname: Yang
authors.forename: Wei
authors.initials: W
authors.lastname: Jiang
authors.forename: Da
authors.initials: D
authors.lastname: Pang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D044466:Asian People / Q000235:genetics; D001943:Breast Neoplasms / Q000208:ethnology / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D002681:China; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D020381:Interleukin-17 / Q000235:genetics*; D015810:Linkage Disequilibrium; D008875:Middle Aged; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide
chemical_list: C440021:IL17F protein, human; D020381:Interleukin-17