title: The effect of fresh bone marrow cells on reconstruction of mouse calvarial defect combined with calvarial osteoprogenitor cells and collagen-apatite scaffold.
abstract: Fresh bone marrow cells have already exhibited its advantages as osteogenic donor cells, but the combination between fresh bone marrow cells and other donor cells utilized for bone healing has not been fully explored. To highlight the impact of fresh bone marrow cells on scaffold-based bone regeneration, single or a combination of calvarial osteoprogenitor cells (OPCs) and bone marrow cells (BMCs) were used as donor cells combined with collagen-apatite scaffold for calvarial defect healing. The host and donor contributions to bone formation were assessed using histological and GFP imaging analysis. Although the amount of new bone formed by different cell sources did not show significant differences, the origin of the bone formation in the defects mainly depended on the types of donor cells employed: when only calvarial OPCs were used as donor cells, a donor-derived bone healing instead of host-derived bone ingrowth was observed; when only fresh BMCs were loaded, the host bone could grow into the defect along the lamellar structure of the scaffolds, but the amount of new bone formed was significantly lower than the defect loaded with calvarial OPCs only. The combination of calvarial OPCs and fresh BMCs had similar amount of new bone formation as the group loaded with calvarial osteoprogenitors alone, but did not induce any host-derived bone formation. These results provide compelling evidence of the importance of fresh BMCs to induce host-implant integration in bone tissue engineering.
authors.affiliation: Department of Chemical, Materials and Biomolecular Engineering, University of Connecticut, Storrs, CT, 06269, USA.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Yu
authors.forename: Liping
authors.initials: L
authors.lastname: Wang
authors.forename: Fei
authors.initials: F
authors.lastname: Peng
authors.forename: Xi
authors.initials: X
authors.lastname: Jiang
authors.forename: Zengmin
authors.initials: Z
authors.lastname: Xia
authors.forename: Jianping
authors.initials: J
authors.lastname: Huang
authors.forename: David
authors.initials: D
authors.lastname: Rowe
authors.forename: Mei
authors.initials: M
authors.lastname: Wei
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D001031:Apatites / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects; D016026:Bone Marrow Transplantation; D003094:Collagen / Q000494:pharmacology*; D004195:Disease Models, Animal; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism; D051379:Mice; D010010:Osteoclasts / Q000166:cytology / Q000187:drug effects; D019919:Prosthesis Implantation; D051381:Rats; D044968:Regenerative Medicine / Q000379:methods*; D012886:Skull / Q000000981:diagnostic imaging / Q000473:pathology*; D013194:Staining and Labeling; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects; D000071681:Tartrate-Resistant Acid Phosphatase; D054457:Tissue Scaffolds / Q000737:chemistry*; D055114:X-Ray Microtomography
chemical_list: D001031:Apatites; D007527:Isoenzymes; D049452:Green Fluorescent Proteins; D003094:Collagen; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase