title: Phyto-synthesis of silver nanoscale particles using Morinda citrifolia L. and its inhibitory activity against human pathogens.
abstract: Leaf extract of Morinda citrifolia L. was assessed for the synthesis of silver nanoscale particles under different temperature and reaction time. Synthesized nanoscale (MCAgNPs) particles were confirmed by analysing the excitation of surface plasmon resonance (SPR) using UV-visible spectrophotometer at 420 nm. Further SEM, HRTEM analysis confirmed the range of particle size between 10 and 60 nm and SEAD pattern authorizes the face centered cubic (fcc) crystalline nature of the MCAgNPs. Fourier transform infrared spectrum (FTIR) of synthesized MCAgNPs confirms the presence of high amount of phenolic compounds in the plant extract which may possibly influence the reduction process and stabilization of nanoparticles. Further, inhibitory activity of MCAgNPs and plant extract were tested against human pathogens like Eschericia coli, Pseudomonas aeroginosa, Klebsiella pneumoniae, Enterobacter aerogenes, Bacillus cereus and Enterococci sp. The results indicated that the MCAgNPs showed moderate inhibitory actions against human pathogens than crude plant extract, demonstrating its antimicrobial value against pathogenic diseases.
authors.affiliation: Department of Biotechnology, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620024, India.
authors.forename: Gnanasekar
authors.initials: G
authors.lastname: Sathishkumar
authors.forename: Chandrakasan
authors.initials: C
authors.lastname: Gobinath
authors.forename: Karuppiah
authors.initials: K
authors.lastname: Karpagam
authors.forename: Vedagiri
authors.initials: V
authors.lastname: Hemamalini
authors.forename: Kumpati
authors.initials: K
authors.lastname: Premkumar
authors.forename: Sivaperumal
authors.initials: S
authors.lastname: Sivaramakrishnan
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001409:Bacillus cereus / Q000187:drug effects; D021902:Enterobacter aerogenes / Q000187:drug effects; D016983:Enterococcus / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D006801:Humans; D007711:Klebsiella pneumoniae / Q000187:drug effects; D053768:Metal Nanoparticles / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D032066:Morinda / Q000737:chemistry*; D010316:Particle Size; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012834:Silver / Q000737:chemistry / Q000494:pharmacology*; D013499:Surface Properties
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D012834:Silver