title: Novel glutamate dehydrogenase genes show increased transcript and protein abundances in mature tomato fruits.
abstract: NAD(P)H-glutamate dehydrogenase (GDH, EC 1.4.1.3) contributes to the control of glutamate homeostasis in all living organisms. In bacteria and animals, GDH is a homohexamer allosterically regulated, whereas in plants NADH-GDH (EC 1.4.1.2) is also found as heterohexamer of α- and β-subunits, but its regulation remains undefined. In tomato (Solanum lycopersicum), GDH activity increases during the fruit ripening along with the content of free glutamate, the most abundant amino acid of ripe fruit involved in conferring the genuine tomato flavour. In this work, novel Slgdh-NAD genes were identified in the recently deciphered tomato genome: three encoding the α-subunit (Slgdh-NAD;A1-3) and one additional gene encoding the β-subunit of GDH (Slgdh-NAD;B1) isolated from a genomic library. These genes are located in different chromosomes. Slgdh-NAD;A1-3 show conserved structures, whereas Slgdh-NAD;B1 includes a novel 5'-untranslated exon. Slgdh-NAD;A1-3 transcripts were detected in all tomato tissues examined, showing the highest levels in mature green fruits, contrasting with Slgdh-NAD;B1 transcripts which were detected mainly in roots or in mature fruits when treated with glutamate, NaCl or salicylic acid. Analyses of GDH activity and protein distribution in different tissues of the Micro-Tom cultivar showed that only the active homohexamer of GDH β-subunits was detected in roots while heterohexamers of GDH α- and β-subunits were found in fruits. These results indicate that GDH β-subunit could modulate the heteromeric isoforms of GDH in response to the environment and physiology of the tomato fruit. This information is relevant to manipulate glutamate contents in tomato fruits genetically.
authors.affiliation: Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha, 531, S2002LRK Rosario, Argentina.
authors.forename: Gisela
authors.initials: G
authors.lastname: Ferraro
authors.forename: Santiago
authors.initials: S
authors.lastname: Bortolotti
authors.forename: Pablo
authors.initials: P
authors.lastname: Mortera
authors.forename: Armin
authors.initials: A
authors.lastname: Schlereth
authors.forename: Mark
authors.initials: M
authors.lastname: Stitt
authors.forename: Fernando
authors.initials: F
authors.lastname: Carrari
authors.forename: Laura
authors.initials: L
authors.lastname: Kamenetzky
authors.forename: Estela M
authors.initials: EM
authors.lastname: Valle
mesh_terms: D000595:Amino Acid Sequence; D002681:China; D005638:Fruit / Q000201:enzymology*; D015971:Gene Expression Regulation, Enzymologic; D017343:Genes, Plant; D005969:Glutamate Dehydrogenase / Q000235:genetics* / Q000378:metabolism*; D018698:Glutamic Acid / Q000378:metabolism*; D018551:Solanum lycopersicum / Q000201:enzymology* / Q000235:genetics*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D010940:Plant Proteins; D018698:Glutamic Acid; D005969:Glutamate Dehydrogenase