title: The differential clinical impact of human coronavirus species in children with cystic fibrosis.
abstract: We investigated the clinical impact of human coronaviruses (HCoV) OC43, 229E, HKU1 and NL63 in pediatric patients with cystic fibrosis (CF) during routine and exacerbation visits. A total of 408 nasopharyngeal aspirate samples were obtained from 103 patients over a 1-year period. Samples positive for HCoV were submitted for nucleotide sequencing to determine the species. Nineteen samples (4.65%) were positive for HCoV, of which 8 were positive for NL63, 6 for OC43, 4 for HKU1, and 1 for 229E. Identification of HCoV was not associated with an increased rate of respiratory exacerbations, but NL63-positive patients had higher exacerbation rates than patients who were positive for other HCoV species.
authors.affiliation: Laboratório de Virologia, LIMHC-FMUSP, Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, Brazil. vicres@usp.br
authors.forename: Luiz Vicente Ribeiro Ferreira
authors.initials: LV
authors.lastname: da Silva Filho
authors.forename: Rodrigo Melim
authors.initials: RM
authors.lastname: Zerbinati
authors.forename: Adriana Fumie
authors.initials: AF
authors.lastname: Tateno
authors.forename: Lucy Vilas
authors.initials: LV
authors.lastname: Boas
authors.forename: Marina Buarque
authors.initials: MB
authors.lastname: de Almeida
authors.forename: José Eduardo
authors.initials: JE
authors.lastname: Levi
authors.forename: Jan Felix
authors.initials: JF
authors.lastname: Drexler
authors.forename: Christian
authors.initials: C
authors.lastname: Drosten
authors.forename: Cláudio Sérgio
authors.initials: CS
authors.lastname: Pannuti
mesh_terms: D000293:Adolescent; D001483:Base Sequence; D002648:Child; D002675:Child, Preschool; D017934:Coronavirus / Q000145:classification* / Q000235:genetics; D018352:Coronavirus Infections / Q000150:complications* / Q000821:virology*; D003550:Cystic Fibrosis / Q000150:complications*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008969:Molecular Sequence Data; D009305:Nasopharynx / Q000821:virology; D010802:Phylogeny; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics
chemical_list: D012324:RNA-Dependent RNA Polymerase