title: Surfactant-assisted pretreatment and enzymatic hydrolysis of spent mushroom compost for the production of sugars.
abstract: Spent mushroom compost (SMC), a byproduct of commercial mushroom cultivation, poses serious environmental problems that have hampered the growth of this important agro-industry. In an effort to develop new applications for SMC, we explored its use as a feedstock for bioethanol production. SMC constitutes approximately 30%w/w polysaccharides, 66% of which is glucan. Following dilute-acid pretreatment and enzymatic hydrolysis, both in the presence of PEG 6000, 97% of glucan and 44% of xylan in SMC were converted into the corresponding monosaccharides. Incorporation of PEG 6000 reduced the cellulase requirement by 77%. Zwittergent 3-12 and 3-14 also significantly increased the efficacy of acid pretreatment and enzymatic hydrolysis. The use of SMC in bioethanol production represents a potential mitigation solution for the critical environmental issues associated with the stockpiling of the major byproduct of the mushroom industry.
authors.affiliation: Expansyn Technologies, Inc., 200 Innovation Boulevard, Suite 258-B, State College, PA 16803, USA.
authors.forename: N U S
authors.initials: NU
authors.lastname: Kapu
authors.forename: M
authors.initials: M
authors.lastname: Manning
authors.forename: T B
authors.initials: TB
authors.lastname: Hurley
authors.forename: J
authors.initials: J
authors.lastname: Voigt
authors.forename: D J
authors.initials: DJ
authors.lastname: Cosgrove
authors.forename: C P
authors.initials: CP
authors.lastname: Romaine
mesh_terms: D000363:Agaricales / Q000737:chemistry* / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D006868:Hydrolysis; D009005:Monosaccharides / Q000096:biosynthesis*; D009097:Multienzyme Complexes / Q000737:chemistry*; D012037:Refuse Disposal / Q000379:methods; D012988:Soil Microbiology
chemical_list: D009005:Monosaccharides; D009097:Multienzyme Complexes; D000431:Ethanol