title: Staphylococcal superantigen-like protein 10 binds to phosphatidylserine and apoptotic cells.
abstract: Staphylococcal superantigen-like proteins (SSLs) are a family of exoproteins that have structural similarities to staphylococcal superantigens. Although SSLs do not have superantigenic activity, some of them have been reported to bind to host immune related molecules and they have been implicated in immune evasion by S. aureus. In this study, we showed that SSL10 is capable of binding to phospholipids. SSL10 bound to phosphatidylserine (PS) containing liposome, but not to phosphatidylcholine liposome. SSL10, but not SSL7, bound to PS containing liposome, suggesting that SSL10 specifically binds to PS. Analysis of PS binding ability among recombinant truncated SSL10 fragments revealed that the β-barrel in the N-terminal oligonucleotide/oligosaccharide-binding (OB)-fold domain contributes to PS binding capacity. Fluorescein isothiocyanate labeled OB-fold of SSL10 stained hydrogen peroxide treated Jurkat cells. Annexin V is widely utilized for detection of apoptosis. Unlike annexin V, the OB-fold domain of SSL10 also bound to apoptotic cells in the presence of EDTA, suggesting that the OB-fold of SSL10 recognizes PS and apoptotic cells in a Ca(2+)  independent manner. These findings suggest SSL10 and its derived peptides may be a novel detection tool for apoptotic cells.
authors.affiliation: Department of Molecular Health Sciences, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1, Tanabe-Dori, Mizuho-ku, Nagoya, Japan. s-itoh@phar.nagoya-cu.ac.jp
authors.forename: Saotomo
authors.initials: S
authors.lastname: Itoh
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Yokoyama
authors.forename: Chizuko
authors.initials: C
authors.lastname: Murase
authors.forename: Takemasa
authors.initials: T
authors.lastname: Takii
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Tsuji
authors.forename: Kikuo
authors.initials: K
authors.lastname: Onozaki
mesh_terms: D017209:Apoptosis; D001426:Bacterial Proteins / Q000378:metabolism*; D006801:Humans; D019169:Jurkat Cells; D008081:Liposomes / Q000378:metabolism; D010718:Phosphatidylserines / Q000378:metabolism*; D011485:Protein Binding; D013211:Staphylococcus aureus / Q000737:chemistry*; D018089:Superantigens / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D008081:Liposomes; D010718:Phosphatidylserines; D018089:Superantigens