title: Two-color DNA nanoprobe of intracellular dynamics.
abstract: We have developed a correlation microscopy technique to follow the dynamics of quantum dot labeled DNA within living cells. The temporal correlation functions of the labels reflect the fluctuations of the DNA nanoprobe as a result of its interactions with the cellular environment. They provide a sensitive measure for the length of the probe on the scale of a persistence length (∼50 nm) and reveal strong nonthermal dynamics of the cell. These results pave the way for dynamic observations of DNA conformational changes in vivo.
authors.affiliation: Department of Chemical and Physical Sciences, University of Toronto Mississauga, Mississauga, ON L5L 1C6. josh.milstein@utoronto.ca
authors.forename: Joshua N
authors.initials: JN
authors.lastname: Milstein
authors.forename: Mike
authors.initials: M
authors.lastname: Chu
authors.forename: Krishnan
authors.initials: K
authors.lastname: Raghunathan
authors.forename: Jens-Christian
authors.initials: JC
authors.lastname: Meiners
mesh_terms: D003116:Color; D015342:DNA Probes; D036103:Nanotechnology
chemical_list: D015342:DNA Probes