title: [Glucose effect in the expression of endothelial lipase in human endothelial cells and in patients with diabetes mellitus type 2].
abstract: BACKGROUND
  Endothelial Lipase (EL), enzyme that modulates HDL metabolism, is overregulated by inflammatory-cytokines. Type 2 Diabetes (DM2) has been associated with a subclinical inflammation, so it has been ruled that these patients could have high levels of EL. The objectives of the research are to determine the effect of glucose in the expression of EL in culturing cells and evaluate the relation between the levels of EL and the metabolic control in patients with DM2.
  
  
  METHODS
  During 24 hours, human endothelial cells (HUVEC) were stimulated with different concentrations of glucose (5.5, 25 and 50 mmol/L), the effect was evaluated over the expression of EL. In DM2 patients levels of EL, glucose and HbA1c were measured. We had a control group (8) to determine the levels of enzyme. EL was measured by immune transference, and the results were expressed by arbitrary units(AU).
  
  
  RESULTS
  In HUVEC cells, the expression of EL was directly proportional extracellular glucose (p < 0.05). 24 diabetic patients were evaluated (15 females and 9 males) average age from 60 ± 9,7 years old. The studied group showed levels of EL bigger than the control group (14911AU and 10250, 18AU) respectively (p < 0.05). We found no relation between glucose, HbA1c and EL.
  
  
  CONCLUSIONS
  In HUVEC cells there is a direct relation between extracell glucose and EL. The diabetic patients had higher levels of EL than the control group, but these was not related with glucose or HbA1c, these shows the existence of other factors that participate in the increasement of EL.
authors.affiliation: Departamento Nutrición, Facultad Medicina, Universidad de Chile, Santiago, Chile. cpierart@med.uchile.cl
authors.forename: C
authors.initials: C
authors.lastname: Pierart
authors.forename: V
authors.initials: V
authors.lastname: Serrano
authors.forename: L
authors.initials: L
authors.lastname: Rubio
authors.forename: R
authors.initials: R
authors.lastname: Ebensperger
authors.forename: R
authors.initials: R
authors.lastname: Foncea
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001786:Blood Glucose / Q000378:metabolism; D002478:Cells, Cultured; D003924:Diabetes Mellitus, Type 2 / Q000201:enzymology*; D042783:Endothelial Cells / Q000187:drug effects* / Q000201:enzymology*; D005260:Female; D005947:Glucose / Q000494:pharmacology*; D006442:Glycated Hemoglobin / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008049:Lipase / Q000096:biosynthesis* / Q000097:blood; D008297:Male; D008875:Middle Aged
chemical_list: D001786:Blood Glucose; D005947:Glucose; D006442:Glycated Hemoglobin; D008049:Lipase