title: Effects of 2,4-dimethoxyphenyl biguanide on glutathione system activity in rat tissues in brain ischemia-reperfusion.
abstract: We studied the effects of dimethoxyphenyl biguanide on glutathione peroxidase and glutathione reductase activities, level of reduced glutathione in rat tissues, and activity of some NADPH-regenerating enzymes (glucose-6-phosphate dehydrogenase and NADP-isocitrate dehydrogenase) under conditions of ischemia-reperfusion of the brain. Administration of this biguanide derivative under pathological conditions led to a decrease in the analyzed parameters (elevated under conditions of ischemia-reperfusion) in the serum and brain tissue. The results attest to less pronounced mobilization of the glutathione system (compared to pathological state) due to antioxidant and protective properties of 2,4-dimethoxyphenyl biguanide under conditions of ischemic tissue damage associated with oxidative stress.
authors.affiliation: Department of Medical Biochemistry and Microbiology, Voronezh State University, Moscow, Russia. safonova@bio.vsu.ru
authors.forename: O A
authors.initials: OA
authors.lastname: Safonova
authors.forename: T N
authors.initials: TN
authors.lastname: Popova
authors.forename: L F
authors.initials: LF
authors.lastname: Panchenko
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001645:Biguanides / Q000494:pharmacology*; D001921:Brain / Q000098:blood supply / Q000187:drug effects / Q000201:enzymology*; D002545:Brain Ischemia / Q000097:blood / Q000201:enzymology* / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000097:blood / Q000378:metabolism; D005978:Glutathione / Q000097:blood / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000097:blood / Q000378:metabolism*; D005980:Glutathione Reductase / Q000097:blood / Q000378:metabolism*; D007521:Isocitrate Dehydrogenase / Q000097:blood / Q000378:metabolism; D008297:Male; D010084:Oxidation-Reduction; D051381:Rats; D015427:Reperfusion Injury / Q000097:blood / Q000201:enzymology* / Q000378:metabolism
chemical_list: C573825:2,4-dimethoxyphenyl biguanide; D000975:Antioxidants; D001645:Biguanides; D007521:Isocitrate Dehydrogenase; C039355:isocitrate dehydrogenase (NADP+); D005954:Glucosephosphate Dehydrogenase; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005978:Glutathione