title: Transcription expression and clinical significance of dishevelled-3 mRNA and δ-catenin mRNA in pleural effusions from patients with lung cancer.
abstract: OBJECTIVE
  To evaluate diagnostic utility of Dishevelled-3 (DVL-3) mRNA and δ-catenin mRNA expression in pleural effusions of patients with lung cancer.
  
  
  METHODS
  DVL-3 mRNA and δ-catenin mRNA levels were assessed by performing RT-PCR on pleural effusion specimens from patients with lung cancer (n = 75) and with lung benign disease (n = 51).
  
  
  RESULTS
  The expressions of DVL-3 mRNA and δ-catenin mRNA were significantly higher in malignant than in benign lung disease (P < 0.01) and were obviously higher than cytology in adenocarcinoma (P < 0.01). In single use, DVL-3 mRNA had the highest specificity (94.1%) and PPV (95.7%), whereas δ-catenin mRNA had the highest sensitivity (92.0%) and NPV (88.5%). When combinations of markers were evaluated together, DVL-3 mRNA and δ-catenin mRNA gave a high-diagnostic performance: sensitivity of 100.0%, NPV of 100.0%, and accuracy of 96.0%, respectively.
  
  
  CONCLUSIONS
  As molecular markers of detecting pleural micrometastasis, DVL-3 mRNA and δ-catenin mRNA are helpful to diagnose the cancer cells in pleural effusions of patients with lung cancer.
authors.affiliation: Department of Pathology, The First Affiliated Hospital and College of Basic Medical Sciences, China Medical University, Shenyang 110001, China.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Li
authors.forename: Shu-Li
authors.initials: SL
authors.lastname: Liu
authors.forename: Na
authors.initials: N
authors.lastname: Cha
authors.forename: Yu-Jie
authors.initials: YJ
authors.lastname: Zhao
authors.forename: Shao-Cheng
authors.initials: SC
authors.lastname: Wang
authors.forename: Wei-Nan
authors.initials: WN
authors.lastname: Li
authors.forename: En-Hua
authors.initials: EH
authors.lastname: Wang
authors.forename: Guang-Ping
authors.initials: GP
authors.lastname: Wu
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics* / Q000378:metabolism; D000230:Adenocarcinoma / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism; D000077192:Adenocarcinoma of Lung; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D016022:Case-Control Studies; D051177:Catenins / Q000235:genetics* / Q000378:metabolism; D000072261:Dishevelled Proteins; D005260:Female; D015870:Gene Expression; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D061206:Neoplasm Micrometastasis / Q000175:diagnosis* / Q000235:genetics; D010750:Phosphoproteins / Q000235:genetics* / Q000378:metabolism; D010996:Pleural Effusion / Q000235:genetics / Q000378:metabolism; D011014:Pneumonia / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism; D000095002:Delta Catenin
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D014408:Biomarkers, Tumor; D051177:Catenins; C000606584:DVL3 protein, human; D000072261:Dishevelled Proteins; D010750:Phosphoproteins; D012333:RNA, Messenger; D000095002:Delta Catenin