title: Assessment of Bdellovibrio bacteriovorus 109J killing of Moraxella bovis in an in vitro model of infectious bovine keratoconjunctivitis.
abstract: The objective of this study was to determine the potential of Bdellovibrio bacteriovorus 109J as an alternative non-chemotherapeutic treatment of infectious bovine keratoconjunctivitis (IBK). To accomplish this, various parameters of B. bacteriovorus predation of Moraxella bovis were determined in vitro. Initial passage of B. bacteriovorus using M. bovis as prey required 10 d for active cultures to develop compared with 2 d for culture on normal Escherichia coli prey; however by the 5th passage, time to active predatory morphology was reduced to 2 d. This high passage B. bacteriovorus culture [1 × 10(10) plaque forming units (PFU)/mL] killed 76% of M. bovis [1 × 10(7) colony forming units (CFU)/mL] present in suspension broth in a 4 h assay. The minimal level of M. bovis supporting B. bacteriovorus predation was 1 × 10(4) CFU/mL. To assess the ability of B. bacteriovorus to kill M. bovis on an epithelial surface mimicking IBK, an in vitro assay with Madin-Darby bovine kidney (MDBK) cells inoculated with 4 × 10(7) CFU/mL M. bovis was used. Treatment with a B. bacteriovorus suspension (1.6 × 10(11) PFU/mL) decreased adherence of M. bovis to MDBK cells by 6-fold at 12 h of treatment, as well as decreased the number of unattached M. bovis cells by 1.4-fold. This study demonstrates that B. bacteriovorus has potential as an effective biological control of M. bovis at levels likely present in IBK-infected corneal epithelia and ocular secretions.
authors.affiliation: Department of Veterinary Clinical Sciences, Oklahoma State University, Center for Veterinary Health Sciences, Stillwater, Oklahoma, USA. melanie.boileau@okstate.edu
authors.forename: Mélanie J
authors.initials: MJ
authors.lastname: Boileau
authors.forename: Kenneth D
authors.initials: KD
authors.lastname: Clinkenbeard
authors.forename: John J
authors.initials: JJ
authors.lastname: Iandolo
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D001501:Bdellovibrio / Q000502:physiology*; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D018920:Coculture Techniques; D004285:Dogs; D007637:Keratoconjunctivitis / Q000382:microbiology / Q000662:veterinary*; D007668:Kidney / Q000166:cytology; D016955:Moraxella bovis / Q000502:physiology*