title: The partial isolation of subcellular MHC products which are recognized by alloimmune T lymphocytes.
abstract: H-2 antigens from three murine tumor-cell lines (YAC, EL-4 and P815) were solubilized with triton X-100 and separated by SDS-polyacrylamide gel electrophoresis. Proteins were eluted from gel slices and assayed for inhibition by a target-effector binding (TBC) assay. Fractions inhibiting alloimmune T cells also contained serologically detectable H-2 molecules as judged by a complement-dependent microcytotoxicity-inhibition assay. Fractions containing Moloney cell-surface antigen (MCSA), gp 71, or p30 did not inhibit alloimmune TBC. H-2 antigens were selective for T cells since they failed to inhibit binding of natural killer (NK) cells to NK-sensitive targets, whereas, in the reciprocal experiment, the NK target antigens inhibited NK cells but not alloimmune T cell binding to intact targets. Cross inhibition tests in the T-cell system revealed that the H-2 molecules maintained their specific antigenic structure. These observations suggest that it will be possible to characterize further those parts of the subcellular H-2 preparations reacting with distinct killer T-cell clones.
authors.affiliation: Department of Tumor Biology, Karolinska Institutet, S-104 01 Stockholm 60, Sweden.
authors.forename: J C
authors.initials: JC
authors.lastname: Roder
authors.forename: K
authors.initials: K
authors.lastname: Karre
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000276:immunology*; D045744:Cell Line, Tumor; D003602:Cytotoxicity, Immunologic; D006183:H-2 Antigens / Q000276:immunology*; D007114:Immunization; D007694:Killer Cells, Natural / Q000276:immunology*; D008285:Major Histocompatibility Complex / Q000276:immunology; D051379:Mice; D008979:Moloney murine leukemia virus / Q000276:immunology; D017830:Octoxynol; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000954:Antigens, Surface; D006183:H-2 Antigens; D017830:Octoxynol