title: Beneficial metabolic effects of 2',3',5'-tri-acetyl-N6- (3-hydroxylaniline) adenosine in the liver and plasma of hyperlipidemic hamsters.
abstract: BACKGROUND
  Pharmaceutical research of hyperlipidemia has been commonly pursued using traditional approaches. However, unbiased metabonomics attempts to explore the metabolic signature of hyperlipidemia in a high-throughput manner to understand pathophysiology of the disease process.
  
  
  RESULTS
  As a new way, we performed (1)H NMR-based metabonomics to evaluate the beneficial effects of 2',3',5'-tri-acetyl-N(6)- (3-hydroxylaniline) adenosine (WS070117) on plasma and liver from hyperlipidemic Syrian golden hamsters. Both plasma and liver profiles provided a clearer distinction between the control and hyperlipidemic hamsters. Compared to control animals, hyperlipidemic hamsters showed a higher content of lipids (triglyceride and cholesterol), lactate and alanine together with a lower content of choline-containing compounds (e.g., phosphocholine, phosphatidylcholine, and glycerophosphocholine) and betaine. As a result, metabonomics-based findings such as the PCA and OPLS-DA plotting of metabolic state and analysis of potential biomarkers in plasma and liver correlated well to the assessment of biochemical assays, Oil Red O staining and in vivo ultrasonographic imaging suggesting that WS070117 was able to regulate lipid content and displayed more beneficial effects on plasma and liver than simvastatin.
  
  
  CONCLUSIONS
  This work demonstrates the promise of applying (1)H NMR metabonomics to evaluate the beneficial effects of WS070117 which may be a good drug candidate for hyperlipidemia.
authors.affiliation: State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Sun
authors.forename: Zeqin
authors.initials: Z
authors.lastname: Lian
authors.forename: Chunying
authors.initials: C
authors.lastname: Jiang
authors.forename: Yinghong
authors.initials: Y
authors.lastname: Wang
authors.forename: Haibo
authors.initials: H
authors.lastname: Zhu
mesh_terms: D000241:Adenosine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D001391:Azo Compounds; D015415:Biomarkers / Q000097:blood; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D006224:Cricetinae; D004195:Disease Models, Animal; D006949:Hyperlipidemias / Q000000981:diagnostic imaging / Q000378:metabolism* / Q000473:pathology; D008099:Liver / Q000187:drug effects* / Q000378:metabolism*; D055432:Metabolomics; D025341:Principal Component Analysis; D014280:Triglycerides / Q000097:blood; D014463:Ultrasonography
chemical_list: D001391:Azo Compounds; D015415:Biomarkers; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; C561084:O2',O3',O5'-triacetyl-N6-(3-hydroxylaniline)adenosine; D014280:Triglycerides; C011049:oil red O; D000241:Adenosine