title: miR-495 and miR-551a inhibit the migration and invasion of human gastric cancer cells by directly interacting with PRL-3.
abstract: The phosphatase of regenerating liver-3 (PRL-3) gene is associated with metastasis in gastric cancer, and is believed to play a causative role by promoting tumor cell motility, invasion, and metastasis, but little is known of the mechanisms involved. We previously reported that PRL-3 expression is significantly higher in the tissues of primary gastric carcinomas with peritoneal metastasis. In the present study, we found that two microRNAs (miRNAs), miR-495 and miR-551a, predicted to target PRL-3, are downregulated in gastric carcinoma samples. The validation of this interaction between those two miRNAs and PRL-3 was confirmed by western blotting and quantitative real-time PCR (qPCR) in GC cell lines transfected with miR-495 and miR-551a mimics. Furthermore, the migration and invasion of GC cells were significantly inhibited by transfection with miR-495 or -551a mimics, and the mRNA and protein levels of PRL-3 were reduced in cells overexpressing miR-495 or -551a. Collectively, our findings suggest that miR-495 and miR-551a both act as tumor suppressors by targeting the PRL-3 oncogene and inhibiting gastric cancer cell migration and invasion. The findings of this study contribute to current understanding of the functions of miRNA mimics in GC gene therapy.
authors.affiliation: Department of Gastrointestinal Surgery, The First Affiliated Hospital of Nanchang University, Nanchang 330006, China. Electronic address: lizhengrong08@yahoo.com.cn.
authors.forename: Zhengrong
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Gastrointestinal Surgery, The First Affiliated Hospital of Nanchang University, Nanchang 330006, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Department of Gastrointestinal Surgery, The First Affiliated Hospital of Nanchang University, Nanchang 330006, China. Electronic address: jiezg123@126.com.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Jie
authors.affiliation: Department of Gastrointestinal Surgery, The First Affiliated Hospital of Nanchang University, Nanchang 330006, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Gastrointestinal Surgery, The First Affiliated Hospital of Nanchang University, Nanchang 330006, China.
authors.forename: Yingliang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, China.
authors.forename: Junhe
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Gastrointestinal Surgery, The First Affiliated Hospital of Nanchang University, Nanchang 330006, China.
authors.forename: Guoming
authors.initials: G
authors.lastname: Zhu
authors.affiliation: Department of Gastrointestinal Surgery, The First Affiliated Hospital of Nanchang University, Nanchang 330006, China.
authors.forename: Zhengren
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Pathology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Tu
authors.affiliation: Department of Gastrointestinal Surgery, The First Affiliated Hospital of Nanchang University, Nanchang 330006, China.
authors.forename: Gen
authors.initials: G
authors.lastname: Peng
authors.affiliation: Department of Surgery, Korea University Anam Hospital, South Korea.
authors.forename: Dong-Woo
authors.initials: DW
authors.lastname: Lee
authors.affiliation: Department of Surgery, Korea University Anam Hospital, South Korea.
authors.forename: Sung-Soo
authors.initials: SS
authors.lastname: Park
mesh_terms: D015153:Blotting, Western; D002465:Cell Movement / Q000235:genetics*; D015536:Down-Regulation; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000378:metabolism*; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*
chemical_list: D035683:MicroRNAs; D009363:Neoplasm Proteins; C484730:PTP4A3 protein, human; D017027:Protein Tyrosine Phosphatases