title: Conditional expression of Lodavin, an avidin-tagged LDL receptor, for biotin-mediated applications in vivo.
abstract: Lodavin represents an engineered fusion protein that consists of a cytoplasmic and a transmembrane domain of the human low-density lipoprotein receptor coupled to an extracellular avidin monomer. Biotinylated compounds have been successfully targeted to Lodavin-expressing cells that have been transduced by a Lodavin-containing virus, and the targeting is based on the high affinity between biotin and avidin. We engineered a Rosa26 (R26R) knock-in Lodavin mouse to develop biotin-based applications such as targeted drug delivery, cell purification, and tissue imaging in vivo. A cDNA encoding Lodavin was inserted downstream of a floxed βgeo resistance gene in the R26R locus in embryonic stem cells, and a germ line-derived R26RLodavin mouse line was generated. Efficient removal of the floxed βgeo cassette and conditional activation of Lodavin expression was achieved as a result of crossing the R26RLodavin mice with HoxB7-Cre, Wnt4-Cre, or Tie1-Cre mice. In summary, the R26RLodavin mouse line may provide a useful tool for testing and developing applications with the aid of avidin and biotin interaction.
authors.affiliation: Oulu Centre for Cell-Matrix Research, Biocenter Oulu, Laboratory of Developmental Biology, Department of Medical Biochemistry and Molecular Biology, University of Oulu, Oulu, Finland.
authors.forename: Subramanian
authors.initials: S
authors.lastname: Murugan
authors.forename: Ulla
authors.initials: U
authors.lastname: Saarela
authors.forename: Kari
authors.initials: K
authors.lastname: Airenne
authors.forename: Jingdong
authors.initials: J
authors.lastname: Shan
authors.forename: Ilya
authors.initials: I
authors.lastname: Skovorodkin
authors.forename: Seppo
authors.initials: S
authors.lastname: Ylä-Herttuala
authors.forename: Seppo J
authors.initials: SJ
authors.lastname: Vainio
mesh_terms: D000818:Animals; D001360:Avidin / Q000235:genetics* / Q000378:metabolism; D001710:Biotin / Q000378:metabolism*; D019921:Biotinylation; D003433:Crosses, Genetic; D016503:Drug Delivery Systems; D053595:Embryonic Stem Cells; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D055879:Gene Knock-In Techniques; D005822:Genetic Vectors; D018398:Homeodomain Proteins / Q000235:genetics; D006801:Humans; D019426:Integrases; D007668:Kidney / Q000166:cytology* / Q000196:embryology; D051379:Mice; D008822:Mice, Transgenic; D023421:Models, Animal; D017434:Protein Structure, Tertiary; D022661:RNA, Untranslated / Q000235:genetics* / Q000378:metabolism; D042810:Receptor, TIE-1 / Q000235:genetics; D011973:Receptors, LDL / Q000235:genetics* / Q000378:metabolism; D011993:Recombinant Fusion Proteins; D060528:Wnt4 Protein / Q000235:genetics
chemical_list: C110853:Gt(ROSA)26Sor non-coding RNA, mouse; D018398:Homeodomain Proteins; C093253:Hoxb7 protein, mouse; C573412:LDLR protein, human; D022661:RNA, Untranslated; D011973:Receptors, LDL; D011993:Recombinant Fusion Proteins; C555581:WNT4 protein, human; D060528:Wnt4 Protein; D001360:Avidin; D001710:Biotin; D042810:Receptor, TIE-1; C045073:Cre recombinase; D019426:Integrases