title: Phagocytosis and endocytosis of silver nanoparticles induce interleukin-8 production in human macrophages.
abstract: Phagocytosis or endocytosis by macrophages is critical to the uptake of fine particles, including nanoparticles, in order to initiate toxic effects in cells. Here, our data enhance the understanding of the process of internalization of silver nanoparticles by macrophages. When macrophages were pre-treated with inhibitors to phagocytosis, caveolin-mediated endocytosis, or clathrin-mediated endocytosis, prior to exposure to silver nanoparticles, Interleukin-8 (IL-8) production was inhibited. Although cell death was not reduced, the inflammatory response by macrophages was compromised by phagocytosis and endocytosis inhibitors.
authors.affiliation: Department of Microbiology, Brain Korea 21 Project for Medical Science, Institute for Immunology and Immunological Diseases, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Seungjae
authors.initials: S
authors.lastname: Kim
authors.forename: In-Hong
authors.initials: IH
authors.lastname: Choi
mesh_terms: D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004705:Endocytosis / Q000502:physiology*; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D053768:Metal Nanoparticles / Q000737:chemistry*; D010587:Phagocytosis / Q000502:physiology*; D012834:Silver / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D016209:Interleukin-8; D012834:Silver