title: Nicotine-induced changes of brain β-endorphin.
abstract: A consensus has emerged that endogenous opioid peptides and their receptors play an important role in the psychoactive properties of nicotine. Although behavioral studies have shown that β-endorphin contributes to the rewarding and emotional effects of nicotine, whether the drug alters the function of brain endorphinergic neurons is not fully explored. These studies investigated the effect of acute, 1mg/kg, sc, and chronic, daily injection of 1mg/kg, sc, for 14 days, administration of free base nicotine on brain β-endorphin and its precursor proopiomelanocortin (POMC). Acute and chronic treatment with nicotine decreased β-endorphin content in hypothalamus, the principal site of β-endorphin producing neurons in the brain, and in the endorphinergic terminal fields in striatum and hippocampus. The acute effect of nicotine on β-endorphin was reversed by the nicotinic antagonist mecamylamine and the dopamine antagonist haloperidol, indicating pharmacological specificity and involvement of dopamine D2-like receptors. Similar observations were made in prefrontal cortex. POMC mRNA in hypothalamus and prefrontal cortex was unchanged following acute nicotine, but it decreased moderately with chronic treatment. The nicotine treatments had no effect on pituitary and plasma β-endorphin. Taken together, these results could be interpreted to indicate that nicotine alters the synthesis and release of β-endorphin in the limbic brain in vivo. Altered endorphinergic function may contribute to the behavioral effects of acute and chronic nicotine treatment and play a role in nicotine addiction.
authors.affiliation: Department of Pharmacology, College of Medicine, The Ohio State University, Columbus, OH 43210, USA. neff.6@osu.edu
authors.forename: K P
authors.initials: KP
authors.lastname: Gudehithlu
authors.forename: A-M
authors.initials: AM
authors.lastname: Duchemin
authors.forename: G A
authors.initials: GA
authors.lastname: Tejwani
authors.forename: N H
authors.initials: NH
authors.lastname: Neff
authors.forename: M
authors.initials: M
authors.lastname: Hadjiconstantinou
mesh_terms: D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects*; D003342:Corpus Striatum / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D009474:Neurons / Q000378:metabolism; D009538:Nicotine / Q000494:pharmacology*; D018722:Nicotinic Agonists / Q000494:pharmacology*; D017397:Prefrontal Cortex / Q000187:drug effects / Q000378:metabolism; D011333:Pro-Opiomelanocortin / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D017448:Receptors, Dopamine D2 / Q000187:drug effects / Q000378:metabolism; D001615:beta-Endorphin / Q000378:metabolism*
chemical_list: D018722:Nicotinic Agonists; D012333:RNA, Messenger; D017448:Receptors, Dopamine D2; D001615:beta-Endorphin; D011333:Pro-Opiomelanocortin; D009538:Nicotine