title: Reducing progoitrin and enriching glucoraphanin in Brassica napus seeds through silencing of the GSL-ALK gene family.
abstract: The hydrolytic products of glucosinolates in brassica crops are bioactive compounds. Some glucosinolate derivatives such as oxazolidine-2-thione from progoitrin in brassica oilseed meal are toxic and detrimental to animals, but some isothiocyanates such as sulforaphane are potent anti-carcinogens that have preventive effects on several human cancers. In most B. rapa, B. napus and B. juncea vegetables and oilseeds, there is no or only trace amount of glucoraphanin that is the precursor to sulforaphane. In this paper, RNA interference (RNAi) of the GSL-ALK gene family was used to down-regulate the expression of GSL-ALK genes in B. napus. The detrimental glucosinolate progoitrin was reduced by 65 %, and the beneficial glucosinolate glucoraphanin was increased to a relatively high concentration (42.6 μmol g(-1) seed) in seeds of B. napus transgenic plants through silencing of the GSL-ALK gene family. Therefore, there is potential application of the new germplasm with reduced detrimental glucosinolates and increased beneficial glucosinolates for producing improved brassica vegetables.
authors.affiliation: Department of Plant Science, University of Manitoba, Winnipeg R3T2N2, Canada.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Liu
authors.forename: Arvind H
authors.initials: AH
authors.lastname: Hirani
authors.forename: Peter B E
authors.initials: PB
authors.lastname: McVetty
authors.forename: Fouad
authors.initials: F
authors.lastname: Daayf
authors.forename: Carlos F
authors.initials: CF
authors.lastname: Quiros
authors.forename: Genyi
authors.initials: G
authors.lastname: Li
mesh_terms: D053898:Biosynthetic Pathways / Q000235:genetics; D015139:Blotting, Southern; D001937:Brassica / Q000235:genetics* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D003433:Crosses, Genetic; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D017343:Genes, Plant / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics; D005961:Glucosinolates / Q000737:chemistry / Q000378:metabolism*; D007096:Imidoesters / Q000737:chemistry / Q000378:metabolism*; D013058:Mass Spectrometry; D005810:Multigene Family / Q000235:genetics*; D010091:Oximes; D030821:Plants, Genetically Modified; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012639:Seeds / Q000235:genetics*; D013454:Sulfoxides
chemical_list: D005961:Glucosinolates; D007096:Imidoesters; D010091:Oximes; D013454:Sulfoxides; C009048:progoitrin; C119494:glucoraphanin