title: Multi-target siRNA based on DNMT3A/B homologous conserved region influences cell cycle and apoptosis of human prostate cancer cell line TSU-PR1.
abstract: Abnormal genome hypermethylation participates in the tumorigenesis and development of prostate cancer. Prostate cancer cells highly express DNA methyltransferase 3 (DMNT3) family genes, essential for maintaining genome methylation. In the present study, multi-target siRNA, based on the homologous region of the DNMT3 family, was designed for the in vitro investigation of its effects on the proliferation, migration, and invasion of TSU-PR1 prostate cancer cells. The consequential cell-cycle derangement, through DNMT3A/B or only DNMT3B silencing, was partially efficient, without affecting apoptosis. DNMT3A silencing had absolutely no effect on changing TSU-PR1 cell biological behavior. Hence, DNMT3B alone apparently plays a key role in maintaining the unfavorable behavior of prostate-cancer cells, thereby implying its potential significance as a promising therapeutic target, with DNMT3A simply in the role of helper.
authors.affiliation: Department of Urology, First Affiliated Hospital of Medical School, Xi'an Jiaotong University, Xi'an, Shanxi, P.R. China.
authors.forename: Yue-Feng
authors.initials: YF
authors.lastname: Du
authors.forename: Liang
authors.initials: L
authors.lastname: Liang
authors.forename: Ying
authors.initials: Y
authors.lastname: Shi
authors.forename: Qing-Zhi
authors.initials: QZ
authors.lastname: Long
authors.forename: Jin
authors.initials: J
authors.lastname: Zeng
authors.forename: Xin-Yang
authors.initials: XY
authors.lastname: Wang
authors.forename: Da-Lin
authors.initials: DL
authors.lastname: He