title: [Influence of IFN on expression of chemokine receptor CXCR1,  CXCR2 and their ligand IL-8 in the patients with chronic hepatitis B].
abstract: OBJECTIVE
  To study the mRNA levels of chemokine receptor CXCR1, CXCR2 and IL-8 in the patients with chronic hepatitis B and their association with IFN therapy.
  
  
  METHODS
  The mRNA levels of CXCR1, CXCR2 and IL-8 in peripheral blood mononuclear cells(PBMC)of chronic hepatitis B patients were determined by real-time RT-PCR before and during the IFN-α therapy.
  
  
  RESULTS
  The mRNA levels of CXCR1(0.4474 ± 0.0386)and IL-8(1.1897 ± 0.1028)in peripheral blood of the patients with chronic hepatitis B were significantly higher than those in healthy donors(P<0.01). The mRNA level of CXCR2(0.4720 ± 0.0458)was higher than those in healthy donors, but there was no significant differences between the two groups. During the treatment, the mRNA levels of CXCR1, CXCR2 and IL-8 obviously decreased. After treatment for six month, the mRNA level of CXCR1(0.4129 ± 0.0395), CXCR2(0.4461 ± 0.0477) and IL-8(0.8660 ± 0.1307)were significantly lower than those before treatment(P<0.01 or P<0.05). Additionally, the expressive levels of CXCR1, CXCR2 and IL-8 in the high HBV loading group(HBV-DNA>10(6);)were much higher than those in the low HBV loading group(HBV-DNA<10(6);).
  
  
  CONCLUSIONS
  CXCR1 and IL-8 may contribute to hepatic inflammation. Among them, CXCR1, CXCR2 and IL-8 decrease after IFN treatment, which illustrates that IFN-α plays an important role in down-regulating inflammation response, controlling the development of the patients' condition.
authors.affiliation: Department of Aetiology and Immunology, Medicine College, Anhui University of Science and Technology, Huainan 232001, China. bihuijuan1122@163.com
authors.forename: Hui-Juan
authors.initials: HJ
authors.lastname: Bi
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: He-Sheng
authors.initials: HS
authors.lastname: Huang
authors.forename: Jun-Hua
authors.initials: JH
authors.lastname: Liu
mesh_terms: D000328:Adult; D000410:Alanine Transaminase / Q000097:blood; D000998:Antiviral Agents / Q000627:therapeutic use; D002478:Cells, Cultured; D004279:DNA, Viral / Q000097:blood / Q000235:genetics; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D006515:Hepatitis B virus / Q000187:drug effects / Q000235:genetics; D019694:Hepatitis B, Chronic / Q000188:drug therapy* / Q000235:genetics / Q000821:virology; D006801:Humans; D007372:Interferons / Q000627:therapeutic use*; D016209:Interleukin-8 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D023062:Receptors, Interleukin-8A / Q000235:genetics*; D023063:Receptors, Interleukin-8B / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D016896:Treatment Outcome; D019562:Viral Load; D055815:Young Adult
chemical_list: D000998:Antiviral Agents; D004279:DNA, Viral; D016209:Interleukin-8; D023062:Receptors, Interleukin-8A; D023063:Receptors, Interleukin-8B; D007372:Interferons; D000410:Alanine Transaminase