title: Participation of the GABAergic system in the anesthetic effect of Lippia alba (Mill.) N.E. Brown essential oil.
abstract: The objective of this study was to identify the possible involvement of the GABAergic system in the anesthetic effect of Lippia alba essential oil (EO). We propose a new animal model using silver catfish (Rhamdia quelen) exposed to an anesthetic bath to study the mechanism of action of EO. To observe the induction and potentiation of the anesthetic effect of EO, juvenile silver catfish (9.30 ± 1.85 g; 10.15 ± 0.95 cm; N = 6) were exposed to various concentrations of L. alba EO in the presence or absence of diazepam [an agonist of high-affinity binding sites for benzodiazepinic (BDZ) sites coupled to the GABA A receptor complex]. In another experiment, fish (N = 6) were initially anesthetized with the EO and then transferred to an anesthetic-free aquarium containing flumazenil (a selective antagonist of binding sites for BDZ coupled to the GABA A receptor complex) or water to assess recovery time from the anesthesia. In this case, flumazenil was used to observe the involvement of the GABA-BDZ receptor in the EO mechanism of action. The results showed that diazepam potentiates the anesthetic effect of EO at all concentrations tested. Fish exposed to diazepam and EO showed faster recovery from anesthesia when flumazenil was added to the recovery bath (12.0 ± 0.3 and 7.2 ± 0.7, respectively) than those exposed to water (9.2 ± 0.2 and 3.5 ± 0.3, respectively). In conclusion, the results demonstrated the involvement of the GABAergic system in the anesthetic effect of L. alba EO on silver catfish.
authors.affiliation: Departamento de Farmácia Industrial, Universidade Federal de Santa Maria, RS, Brasil.
authors.forename: C G
authors.initials: CG
authors.lastname: Heldwein
authors.forename: L L
authors.initials: LL
authors.lastname: Silva
authors.forename: P
authors.initials: P
authors.lastname: Reckziegel
authors.forename: F M C
authors.initials: FM
authors.lastname: Barros
authors.forename: M E
authors.initials: ME
authors.lastname: Bürger
authors.forename: B
authors.initials: B
authors.lastname: Baldisserotto
authors.forename: C A
authors.initials: CA
authors.lastname: Mallmann
authors.forename: D
authors.initials: D
authors.lastname: Schmidt
authors.forename: B O
authors.initials: BO
authors.lastname: Caron
authors.forename: B M
authors.initials: BM
authors.lastname: Heinzmann
mesh_terms: D000777:Anesthetics / Q000494:pharmacology*; D000818:Animals; D002397:Catfishes / Q000502:physiology*; D003975:Diazepam / Q000494:pharmacology; D005442:Flumazenil / Q000494:pharmacology; D058785:GABA-A Receptor Agonists / Q000494:pharmacology; D058787:GABA-A Receptor Antagonists / Q000494:pharmacology; D032411:Lippia / Q000737:chemistry*; D023421:Models, Animal; D009822:Oils, Volatile / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D011963:Receptors, GABA-A / Q000187:drug effects*
chemical_list: D000777:Anesthetics; D058785:GABA-A Receptor Agonists; D058787:GABA-A Receptor Antagonists; D009822:Oils, Volatile; D011963:Receptors, GABA-A; D005442:Flumazenil; D003975:Diazepam