title: Gene expression profile in the liver of Rana catesbeiana tadpoles exposed to low temperature in the presence of thyroid hormone.
abstract: Amphibian metamorphosis, which is controlled by thyroid hormone (TH), is highly temperature-sensitive. Using real-time PCR, we investigated the gene expression profile in the liver of Rana catesbeiana tadpoles kept at 28 and 4 °C and treated with 5 nM 3,3',5-triiodothyronine (T3). Out of the 48 genes tested, 12 were up-regulated at 4 °C in T3-treated or untreated tadpoles. These included genes involved in energy metabolism, transcription, and translation. Four TH-response genes, including TH receptor β (TRβ) gene, showed no response to T3 at 4 °C. Deiodinase III was the only gene down-regulated at 4 °C. Chromatin immunoprecipitation assays showed that CCAAT/enhancer-binding protein 2 gene activation by cold exposure was associated with an increase in the acetylation of histone H3 at lysine 9, whereas TRβ gene activation by T3 at 28 °C was associated with an increase in the methylation of histone H3 at lysine 36 with no T3-dependent changes in methylation states on cold exposure. Our results suggest that the transfer of TH signal to chromatin modifications on a primary early TH response gene was specifically blocked by exposure to cold.
authors.affiliation: Laboratory of Nutritional Physiology, School of Food and Nutritional Sciences, The University of Shizuoka, Shizuoka 422-8526, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Mochizuki
authors.forename: Toshinao
authors.initials: T
authors.lastname: Goda
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Yamauchi
mesh_terms: D000107:Acetylation; D000818:Animals; D001833:Body Temperature Regulation / Q000187:drug effects / Q000235:genetics*; D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics; D002843:Chromatin / Q000378:metabolism; D003080:Cold Temperature; D020869:Gene Expression Profiling; D006657:Histones / Q000378:metabolism; D007814:Larva / Q000235:genetics / Q000502:physiology; D008099:Liver / Q000378:metabolism*; D008239:Lysine / Q000378:metabolism; D008745:Methylation; D011892:Rana catesbeiana / Q000235:genetics / Q000254:growth & development*; D037042:Thyroid Hormone Receptors beta / Q000235:genetics; D015533:Transcriptional Activation; D014284:Triiodothyronine / Q000494:pharmacology / Q000502:physiology*
chemical_list: D022762:CCAAT-Enhancer-Binding Proteins; D002843:Chromatin; D006657:Histones; D037042:Thyroid Hormone Receptors beta; D014284:Triiodothyronine; D008239:Lysine