title: In vivo evidence of cross-reactivity between cow's milk and soybean proteins in a mouse model of food allergy.
abstract: BACKGROUND
  Cow's milk allergy (CMA) is an important problem worldwide and the development of an in vivo system to study new immunotherapeutic strategies is of interest. Intolerance to soybean formula has been described in CMA patients, but it is not fully understood. In this work, we used a food allergy model in BALB/c mice to study the cross-reactivity between cow's milk protein (CMP) and soy proteins (SP).
  
  
  METHODS
  Mice were orally sensitized with cholera toxin and CMP, and then challenged with CMP or SP to induce allergy. Elicited symptoms, plasma histamine, humoral and cellular immune response were analyzed. Th1- and Th2-associated cytokines and transcription factors were assessed at mucosal sites and in splenocytes. Cutaneous tests were also performed.
  
  
  RESULTS
  We found that the immediate symptoms elicited in CMP-sensitized mice orally challenged with SP were consistent with a plasma histamine increase. The serum levels of CMP-specific IgE and IgG1 antibodies were increased. These antibodies also recognized soy proteins. Splenocytes and mesenteric lymph node cells incubated with CMP or SP secreted IL-5 and IL-13. mRNA expression of Th2-associated genes (IL-5, IL-13, and GATA-3) was upregulated in mucosal samples. In addition, sensitized animals exhibited positive cutaneous tests after the injection of CMP or SP.
  
  
  CONCLUSIONS
  We demonstrate that CMP-sensitized mice, without previous exposure to soy proteins, elicited hypersensitivity signs immediately after the oral administration of SP, suggesting that the immunochemical cross-reactivity might be clinically relevant. This model may provide an approach to further characterize cross-allergenicity phenomena and develop new immunotherapeutic treatments for allergic patients.
authors.affiliation: Laboratorio de Investigaciones del Sistema Inmune, Facultad de Ciencias Exactas, Universidad de La Plata, La Plata, Argentina.
authors.forename: Paola
authors.initials: P
authors.lastname: Smaldini
authors.forename: Renata
authors.initials: R
authors.lastname: Curciarello
authors.forename: Angela
authors.initials: A
authors.lastname: Candreva
authors.forename: María Amanda
authors.initials: MA
authors.lastname: Rey
authors.forename: Carlos Alberto
authors.initials: CA
authors.lastname: Fossati
authors.forename: Silvana
authors.initials: S
authors.lastname: Petruccelli
authors.forename: Guillermo Horacio
authors.initials: GH
authors.lastname: Docena
mesh_terms: D000818:Animals; D003429:Cross Reactions; D016207:Cytokines / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism; D004195:Disease Models, Animal; D005512:Food Hypersensitivity / Q000276:immunology*; D006632:Histamine / Q000097:blood / Q000276:immunology; D007111:Immunity, Cellular; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D008198:Lymph Nodes / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016269:Milk Hypersensitivity / Q000276:immunology*; D008894:Milk Proteins / Q000276:immunology*; D012882:Skin Tests; D030262:Soybean Proteins / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D016207:Cytokines; D007074:Immunoglobulin G; D008894:Milk Proteins; D030262:Soybean Proteins; D014157:Transcription Factors; D007073:Immunoglobulin E; D006632:Histamine