title: Metal cations modulate the bacteriochlorophyll-protein interaction in the light-harvesting 1 core complex from Thermochromatium tepidum.
abstract: The light-harvesting 1 reaction center (LH1-RC) complex from Thermochromatium (Tch.) tepidum exhibits unusual Q(y) absorption by LH1 bacteriochlorophyll-a (BChl-a) molecules at 915nm, and the transition energy is finely modulated by the binding of metal cations to the LH1 polypeptides. Here, we demonstrate the metal-dependent interactions between BChl-a and the polypeptides within the intact LH1-RC complexes by near-infrared Raman spectroscopy. The wild-type LH1-RC (B915) exhibited Raman bands for the C3-acetyl and C13-keto CO stretching modes at 1637 and 1675cm(-1), respectively. The corresponding bands appeared at 1643 and 1673cm(-1) when Ca(2+) was biosynthetically replaced with Sr(2+) (B888) or at 1647 and 1669cm(-1) in the mesophilic counterpart, Allochromatium vinosum. These results indicate the significant difference in the BChl-polypeptide interactions between B915 and B888 and between B915 and the mesophilic counterpart. The removal of the original metal cations from B915 and B888 resulted in marked band shifts of the C3-acetyl/C13-carbonyl νCO modes to ~1645/~1670cm(-1), supporting a model in which the metal cations are involved in the fine-tuning of the hydrogen bonding between the BChl-a and LH1-polypeptides. Interestingly, the interaction modes were almost identical between the Ca(2+)-depleted B915 and Sr(2+)-depleted B888 and between B915 and Ca(2+)-substituted B888, despite the significant differences in their LH1 Q(y) peak positions and the denaturing temperatures, as revealed by differential scanning calorimetry. These results suggest that not only the BChl-polypeptide interactions but some structural origin may be involved in the unusual Q(y) red-shift and the enhanced thermal stability of the LH1-RC complexes from Tch. tepidum.
authors.affiliation: Organization of Advanced Science and Technology, Kobe University, Nada, Kobe, Japan. ykimura@people.kobe-u.ac.jp
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Kimura
authors.forename: Yuta
authors.initials: Y
authors.lastname: Inada
authors.forename: Tomoko
authors.initials: T
authors.lastname: Numata
authors.forename: Teruhisa
authors.initials: T
authors.lastname: Arikawa
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Zhang
authors.forename: Zheng-Yu
authors.initials: ZY
authors.lastname: Wang
authors.forename: Takashi
authors.initials: T
authors.lastname: Ohno
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D001429:Bacteriochlorophylls / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002152:Calorimetry, Differential Scanning; D002412:Cations / Q000378:metabolism*; D002841:Chromatiaceae / Q000378:metabolism*; D045342:Light-Harvesting Protein Complexes / Q000378:metabolism*; D011485:Protein Binding; D019265:Spectroscopy, Near-Infrared; D013059:Spectrum Analysis, Raman; D013324:Strontium / Q000378:metabolism*; D013997:Time Factors
chemical_list: D001426:Bacterial Proteins; D001429:Bacteriochlorophylls; D002412:Cations; D045342:Light-Harvesting Protein Complexes; D002118:Calcium; D013324:Strontium