title: Transformation of agrobacterium using the freeze-thaw method.
abstract: INTRODUCTIONThis protocol describes a method for transforming Agrobacterium with plasmid DNA using a freeze-thaw technique. Although the transformation efficiency for Agrobacterium is lower than that for Escherichia coli, it is possible to obtain adequate numbers of transformants with this technique.
authors.forename: Detlef
authors.initials: D
authors.lastname: Weigel
authors.forename: Jane
authors.initials: J
authors.lastname: Glazebrook