title: Anti-human hepatoma Hep-G2 proliferative, apoptotic, and antimutagenic activity of tagitinin C from Tithonia diversifolia leaves.
abstract: Tagitinin C, a major sesquiterpenoid, was isolated from the leaves of Tithonia diversifolia. The high morbidity and mortality rate of hepatoma in Taiwan motivated our interest in the investigation of tagitinin C's mechanism against the human hepatocellular carcinoma. The methanolic extract of leaves of T. diversifolia (TDM) and tagitinin C were found to have cytotoxic activities against human hepatoma Hep-G2 cells in the MTT assay with IC(50) values of 40.0 ± 2.0 and 2.0 ± 0.1 μg/mL, respectively. This compound induced population increase in the sub-G(1) phase and S phase arrest. Treatment with tagitinin C isolated from TDM resulted in activation of both caspase 3 and caspase 8 which suggested that the antiproliferative effect of this compound was caspase-dependent apoptosis. Magnetic resonance techniques indicated that the tumorigenisity of xenografts derived from Hep-G2 cells was retarded by the delivery of tagitinin C (15 μg/mouse/day) relative to the control counterparts.
authors.affiliation: Department of Biomedical Engineering, Yuanpei University, Hsinchu, Taiwan.
authors.forename: May-Hua
authors.initials: MH
authors.lastname: Liao
authors.forename: Yuan-Nuan
authors.initials: YN
authors.lastname: Tsai
authors.forename: Chi-Yu
authors.initials: CY
authors.lastname: Yang
authors.forename: Chi-Long
authors.initials: CL
authors.lastname: Juang
authors.forename: Ming-Yi
authors.initials: MY
authors.lastname: Lee
authors.forename: Li-Hsueh
authors.initials: LH
authors.lastname: Chang
authors.forename: Hsiao-Chuan
authors.initials: HC
authors.lastname: Wen
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects*; D019659:Asteraceae / Q000737:chemistry*; D005260:Female; D056945:Hep G2 Cells; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D018515:Plant Leaves / Q000737:chemistry*; D012717:Sesquiterpenes / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use*; D023041:Xenograft Model Antitumor Assays
chemical_list: D016587:Antimutagenic Agents; D010936:Plant Extracts; D012717:Sesquiterpenes; C012565:tagitinin