title: A rapid method for the determination of honey diastase activity.
abstract: A new rapid method for the determination of honey diastase activity using direct potentiometric principles has been proposed. A platinum redox sensor has been used to quantify the amount of free triiodide released from a starch triiodide complex after starch hydrolysis by honey diastase. The method was tested on honey samples with varying diastase activities. The first 5 min of data for each sample were used for linear regression analysis in order to calculate diastase activity. The new method was compared with classical Schade and commercial Phadebas procedures. The results showed good correlations with both methods and offered a simple method for unit conversion to DN units for diastase activity, making the method suitable for routine analysis.
authors.affiliation: Department of Chemistry, Josip Juraj Strossmayer University of Osijek, F. Kuhača 20, 31000 Osijek, Croatia. nikola.sakac@gmail.com
authors.forename: Nikola
authors.initials: N
authors.lastname: Sakač
authors.forename: Milan
authors.initials: M
authors.lastname: Sak-Bosnar
mesh_terms: D000681:Amylases / Q000378:metabolism*; D057075:Enzyme Assays / Q000191:economics / Q000379:methods*; D006722:Honey; D016014:Linear Models; D011199:Potentiometry; D013997:Time Factors
chemical_list: D000681:Amylases