title: KRAS mutational status of endoscopic biopsies matches resection specimens.
abstract: OBJECTIVE
  This study was performed to determine systematically whether KRAS mutational analysis in biopsy tissue is a reliable indicator of KRAS status in subsequent corresponding resection specimens.
  
  
  METHODS
  30 colorectal cancer (CRC) patients with biopsy and corresponding subsequent surgical resection specimens were studied. KRAS mutational analysis was performed on each biopsy sample as well as two separate samples from each resection specimen by PCR and Sanger sequencing.
  
  
  RESULTS
  Overall, KRAS mutations were identified in 12/30 (40%) of the tumours. There was 100% correlation between biopsy and resection specimens regarding the presence or absence of KRAS mutations. In fact, the same point mutation was identified in both biopsy and corresponding resection specimens in 12/12 (100%) cases. In addition, in two cases, there were two different point mutations detected within the same biopsy specimen.
  
  
  CONCLUSIONS
  This study shows perfect correlation between KRAS mutation status in biopsy and resection specimens from an individual patient, and suggests that biopsy material is adequate for KRAS mutational analysis in CRC patients.
authors.affiliation: Miraca Research Institute, Miraca Life Sciences, Irving, TX, USA.
authors.forename: Qing-Hua
authors.initials: QH
authors.lastname: Yang
authors.forename: Jason
authors.initials: J
authors.lastname: Schmidt
authors.forename: Genvieve
authors.initials: G
authors.lastname: Soucy
authors.forename: Robert
authors.initials: R
authors.lastname: Odze
authors.forename: Liza
authors.initials: L
authors.lastname: Dejesa-Jamanila
authors.forename: Keely
authors.initials: K
authors.lastname: Arnold
authors.forename: Christine
authors.initials: C
authors.lastname: Kuslich
authors.forename: Richard
authors.initials: R
authors.lastname: Lash
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology*; D001706:Biopsy; D003106:Colon / Q000473:pathology; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology*; D005260:Female; D005838:Genotype; D006801:Humans; D009154:Mutation / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016283:Proto-Oncogene Proteins p21(ras); D012007:Rectum / Q000473:pathology; D017422:Sequence Analysis, DNA; D018631:ras Proteins / Q000235:genetics*
chemical_list: C117307:KRAS protein, human; D011518:Proto-Oncogene Proteins; D016283:Proto-Oncogene Proteins p21(ras); D018631:ras Proteins