title: Pancreatic autoantibodies and autoantibodies against goblet cells in pediatric patients with inflammatory bowel disease.
abstract: BACKGROUND
  Significance of pancreatic autoantibodies determined by using exocrine pancreas (PAB) and antibodies against recombinant pancreas antigen (rPAB), as well as the importance of autoantibodies against goblet cells (GAB), is not known in pediatric patients with inflammatory bowel disease (IBD). Our aim was to determine the complex analysis of PAB, rPAB, GAB, antibodies against Saccharomyces cerevisiae, and perinuclear components of neutrophils in pediatric patients with IBD. Moreover, association with NOD2/CARD15 and disease phenotype was determined.
  
  
  METHODS
  A total of 152 pediatric patients (median age 13.9 years) with IBD (103 patients with Crohn disease [CD] and 49 patients with ulcerative colitis [UC]) and 104 controls were included. Serum autoantibodies were determined by indirect immunofluorescence assay. NOD2/CARD15 variants were tested by polymerase chain reaction/restriction fragment length polymorphism.
  
  
  RESULTS
  The presence of PAB and rPAB was significantly higher in CD (34% and 35.9%) and in UC (20.4% and 24.5%) compared with pediatric control cohort (0% and 0%, P<0.0001). In addition, GAB positivity was significantly increased in patients with UC in comparison with CD and controls, respectively (UC, 12.2%; CD, 1.9%; controls, 1.9%; P=0.02). Specificity of PAB and rPAB was 100%; however, sensitivity was low. The combination of PAB and/or antibodies against Saccharomyces cerevisiae/perinuclear components of neutrophils improved the sensitivity of serological markers in CD (87.4%) and in UC (79.6%); specificities were 89.3% and 93.2%, respectively. Pancreatic autoantibodies (PAB, rPAB) and GAB were not related to clinical presentation, medical therapy, or need for surgery in CD or in UC.
  
  
  CONCLUSIONS
  Pancreatic autoantibodies and GAB were specific for IBD, but the sensitivity was limited as well because there was lack of correlation with clinical phenotype. Combinations of these antibodies have shown increased sensitivity; therefore, it may be recommended in the diagnostic procedure of IBD.
authors.affiliation: Department of Pediatrics, Petz Aladár County and Teaching Hospital, Győr, Hungary.
authors.forename: Marta
authors.initials: M
authors.lastname: Kovacs
authors.forename: Peter Laszlo
authors.initials: PL
authors.lastname: Lakatos
authors.forename: Maria
authors.initials: M
authors.lastname: Papp
authors.forename: Silvia
authors.initials: S
authors.lastname: Jacobsen
authors.forename: Eva
authors.initials: E
authors.lastname: Nemes
authors.forename: Marianne
authors.initials: M
authors.lastname: Polgar
authors.forename: Eniko
authors.initials: E
authors.lastname: Solyom
authors.forename: Piroska
authors.initials: P
authors.lastname: Bodi
authors.forename: Agnes
authors.initials: A
authors.lastname: Horvath
authors.forename: Katalin Eszter
authors.initials: KE
authors.lastname: Muller
authors.forename: Kriszta
authors.initials: K
authors.lastname: Molnar
authors.forename: Doloresz
authors.initials: D
authors.lastname: Szabo
authors.forename: Aron
authors.initials: A
authors.lastname: Cseh
authors.forename: Antal
authors.initials: A
authors.lastname: Dezsofi
authors.forename: Andras
authors.initials: A
authors.lastname: Arato
authors.forename: Gabor
authors.initials: G
authors.lastname: Veres
mesh_terms: D000293:Adolescent; D000328:Adult; D019268:Antibodies, Antineutrophil Cytoplasmic / Q000097:blood; D000908:Antibodies, Fungal / Q000097:blood; D001323:Autoantibodies / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D003093:Colitis, Ulcerative / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D003424:Crohn Disease / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D005260:Female; D020397:Goblet Cells / Q000276:immunology*; D006801:Humans; D008297:Male; D009504:Neutrophils / Q000276:immunology; D046790:Pancreas, Exocrine / Q000276:immunology*; D012441:Saccharomyces cerevisiae / Q000276:immunology; D055815:Young Adult
chemical_list: D019268:Antibodies, Antineutrophil Cytoplasmic; D000908:Antibodies, Fungal; D001323:Autoantibodies