title: Nanovolume optimization of protein crystal growth using the microcapillary protein crystallization system.
abstract: The Microcapillary Protein Crystallization System (MPCS) is a microfluidic, plug-based crystallization technology that generates X-ray diffraction-ready protein crystals in nanolitre volumes. In this study, 28 out of 29 (93%) proteins crystallized by traditional vapor diffusion experiments were successfully crystallized by chemical gradient optimization experiments using the MPCS technology. In total, 90 out of 120 (75%) protein/precipitant combinations leading to initial crystal hits from vapor diffusion experiments were successfully crystallized using MPCS technology. Many of the resulting crystals produced high-quality X-ray diffraction data, and six novel protein structures that were derived from crystals harvested from MPCS CrystalCards are reported.
authors.forename: Cory J
authors.initials: CJ
authors.lastname: Gerdts
authors.forename: Glenn L
authors.initials: GL
authors.lastname: Stahl
authors.forename: Alberto
authors.initials: A
authors.lastname: Napuli
authors.forename: Bart
authors.initials: B
authors.lastname: Staker
authors.forename: Jan
authors.initials: J
authors.lastname: Abendroth
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Edwards
authors.forename: Peter
authors.initials: P
authors.lastname: Myler
authors.forename: Wesley
authors.initials: W
authors.lastname: Van Voorhis
authors.forename: Peter
authors.initials: P
authors.lastname: Nollert
authors.forename: Lance J
authors.initials: LJ
authors.lastname: Stewart