title: Association of mesenchymal stem cells with platelet rich plasma on the repair of critical calvarial defects in mice.
abstract: OBJECTIVE
  To evaluate the effects of mesenchymal stem cells (MSC) from eight mice C57BL/6 gfp(+) bone marrows expanded in cultures associated with platelets rich plasma (PRP) deriving from another eight mice, in the repair of critical defects in calvarial bone produced in twenty-four adult isogenic mice C57BL/6.
  
  
  METHODS
  The animals were submitted to a cranial defect of 6.0mm in diameter and divided into two equal experimental groups. Control group did not receive treatment and the treated group received a MSC pellet containing 1.0 x 10(7) cells/mL associated with 50.0 µL of plasma gel containing 1.0 x 10(9) autologous platelets within the defect.
  
  
  RESULTS
  In the treated group was observed process of angiogenesis and bone repair better than control group.
  
  
  CONCLUSIONS
  Mesenchymal stem cells derived from bone marrow of C57BL/6 gfp(+) mice associated with PRP gel applied in bone critical defects produced in calvarial contributes positively to the process of bone repair.
authors.affiliation: Department of Veterinary, Vila Velha University, Espirito Santo, Brazil. betania.monteiro@uvv.br
authors.forename: Betânia Souza
authors.initials: BS
authors.lastname: Monteiro
authors.forename: Ricardo Junqueira
authors.initials: RJ
authors.lastname: Del Carlo
authors.forename: Napoleão Martins
authors.initials: NM
authors.lastname: Argôlo-Neto
authors.forename: Nance Beyer
authors.initials: NB
authors.lastname: Nardi
authors.forename: Pablo Herthel
authors.initials: PH
authors.lastname: Carvalho
authors.forename: Laila de Paula
authors.initials: Lde P
authors.lastname: Bonfá
authors.forename: Pedro César
authors.initials: PC
authors.lastname: Chagastelles
authors.forename: Higo Nasser
authors.initials: HN
authors.lastname: Moreira
authors.forename: Marlene Isabel Vargas
authors.initials: MI
authors.lastname: Viloria
authors.forename: Bianka Souza dos
authors.initials: BS
authors.lastname: Santos
mesh_terms: D053687:Adult Stem Cells / Q000637:transplantation* / Q000648:ultrastructure; D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology* / Q000648:ultrastructure; D001861:Bone Regeneration / Q000502:physiology*; D002478:Cells, Cultured; D004195:Disease Models, Animal; D049452:Green Fluorescent Proteins / Q000235:genetics; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000502:physiology* / Q000648:ultrastructure; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D010012:Osteogenesis / Q000502:physiology; D053657:Platelet-Rich Plasma / Q000502:physiology*; D011897:Random Allocation; D012886:Skull / Q000293:injuries / Q000601:surgery* / Q000648:ultrastructure; D023822:Tissue Engineering / Q000379:methods; D014184:Transplantation, Homologous
chemical_list: D049452:Green Fluorescent Proteins