title: Dendrimer-driven neurotrophin expression differs in temporal patterns between rodent and human stem cells.
abstract: This study reports the use of a nonviral expression system based on polyamidoamine dendrimers for time-restricted neurotrophin overproduction in mesenchymal stem cells and skin precursor-derived Schwann cells. The dendrimers were used to deliver plasmids for brain-derived neurotrophic factor (BDNF) or neurotrophin-3 (NT-3) expression in both rodent and human stem cells, and the timelines of expression were studied. We have found that, despite the fact that transfection efficiencies and protein expression levels were comparable, dendrimer-driven expression in human mesenchymal stem cells was characterized by a more rapid decline compared to rodent cells. Transient expression systems can be beneficial for some neurotrophins, which were earlier reported to cause unwanted side effects in virus-based long-term expression models. Nonviral neurotrophin expression is a biologically safe and accessible alternative to increase the therapeutic potential of autologous adult stem cells and stem cell-derived functional differentiated cells.
authors.affiliation: Department of Clinical Neurosciences, Faculty of Medicine, University of Calgary, Calgary, Canada. shakhbazau@gmail.com
authors.forename: Antos
authors.initials: A
authors.lastname: Shakhbazau
authors.forename: Dzmitry
authors.initials: D
authors.lastname: Shcharbin
authors.forename: Ihar
authors.initials: I
authors.lastname: Seviaryn
authors.forename: Natalya
authors.initials: N
authors.lastname: Goncharova
authors.forename: Svetlana
authors.initials: S
authors.lastname: Kosmacheva
authors.forename: Mihail
authors.initials: M
authors.lastname: Potapnev
authors.forename: Maria
authors.initials: M
authors.lastname: Bryszewska
authors.forename: Ranjan
authors.initials: R
authors.lastname: Kumar
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Biernaskie
authors.forename: Rajiv
authors.initials: R
authors.lastname: Midha
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D002478:Cells, Cultured; D020219:Chondrogenesis / Q000235:genetics / Q000502:physiology; D050091:Dendrimers / Q000737:chemistry*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D009414:Nerve Growth Factors / Q000235:genetics / Q000378:metabolism*; D020933:Neurotrophin 3 / Q000235:genetics / Q000378:metabolism; D010012:Osteogenesis / Q000235:genetics / Q000502:physiology; D010957:Plasmids / Q000235:genetics; D014162:Transfection / Q000379:methods
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D050091:Dendrimers; D009414:Nerve Growth Factors; D020933:Neurotrophin 3