title: Nanovesicle-based bioelectronic nose platform mimicking human olfactory signal transduction.
abstract: We developed a nanovesicle-based bioelectronic nose (NBN) that could recognize a specific odorant and mimic the receptor-mediated signal transmission of human olfactory systems. To build an NBN, we combined a single-walled carbon nanotube-based field effect transistor with cell-derived nanovesicles containing human olfactory receptors and calcium ion signal pathways. Importantly, the NBN took advantages of cell signal pathways for sensing signal amplification, enabling ≈ 100 times better sensitivity than that of previous bioelectronic noses based on only olfactory receptor protein and carbon nanotube transistors. The NBN sensors exhibited a human-like selectivity with single-carbon-atomic resolution and a high sensitivity of 1 fM detection limit. Moreover, this sensor platform could mimic a receptor-meditated cellular signal transmission in live cells. This sensor platform can be utilized for the study of molecular recognition and biological processes occurring at cell membranes and also for various practical applications such as food screening and medical diagnostics.
authors.affiliation: Department of Physics and Astronomy, Seoul National University, Seoul 151-747, Republic of Korea.
authors.forename: Hye Jun
authors.initials: HJ
authors.lastname: Jin
authors.affiliation: School of Chemical and Biological Engineering, Bio-MAX Institute, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Sang Hun
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Department of Chemistry, Soonchunhyang University, Asan 336-745, Republic of Korea.
authors.forename: Tae Hyun
authors.initials: TH
authors.lastname: Kim
authors.affiliation: Department of Physics and Astronomy, Seoul National University, Seoul 151-747, Republic of Korea.
authors.forename: Juhun
authors.initials: J
authors.lastname: Park
authors.affiliation: School of Chemical and Biological Engineering, Bio-MAX Institute, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Hyun Seok
authors.initials: HS
authors.lastname: Song
authors.affiliation: School of Chemical and Biological Engineering, Bio-MAX Institute, Seoul National University, Seoul 151-742, Republic of Korea. Electronic address: thpark@snu.ac.kr.
authors.forename: Tai Hyun
authors.initials: TH
authors.lastname: Park
authors.affiliation: Department of Physics and Astronomy, Seoul National University, Seoul 151-747, Republic of Korea; Department of Biophysics and Chemical Biology, Seoul National University, Seoul 151-747, Republic of Korea. Electronic address: seunghun@snu.ac.kr.
authors.forename: Seunghun
authors.initials: S
authors.lastname: Hong
mesh_terms: D001483:Base Sequence; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D018076:DNA, Complementary / Q000235:genetics; D004867:Equipment Design; D057809:HEK293 Cells; D006801:Humans; D055767:Immobilized Proteins; D037742:Nanotubes, Carbon; D009812:Odorants / Q000032:analysis*; D018035:Receptors, Odorant / Q000235:genetics / Q000502:physiology; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D012903:Smell / Q000502:physiology*
chemical_list: D018076:DNA, Complementary; D055767:Immobilized Proteins; D037742:Nanotubes, Carbon; C514887:OR2AG1 protein, human; D018035:Receptors, Odorant; D011994:Recombinant Proteins