title: Cactin is essential for G1 progression in Toxoplasma gondii.
abstract: Toxoplasma gondii is an obligate intracellular protozoan parasite whose rapid lytic replication cycles define its pathogenicity. We identified a temperature-sensitive growth mutant, FV-P6, which irreversibly arrests before the middle of the G1 stage of the tachyzoite cell cycle. This arrest is caused by a point mutation in a gene conserved across eukaryotes, Cactin, whose product localizes to the nucleus. To elucidate the role of TgCactin we performed genome-wide expression profiling. Besides the expected G1 expression profile, many genes associated with the extracellular state as well as with the bradyzoite cyst stage were identified. Consistent with these profiles were the expression of AP2 transcription factors typically associated with extracellular and bradyzoite stage parasites. This suggests a role for TgCactin in control of gene expression. As TgCactin does not contain any functionally defined domains we reasoned TgCactin exerts its function through interactions with other proteins. In support of this model we demonstrated that TgCactin is present in a protein complex and can oligomerize. Taken together, these results suggest that TgCactin acts as a pivotal protein potentially regulating gene expression at several transition points in parasite development.
authors.affiliation: Department of Biology, Boston College, Chestnut Hill, MA 02467, USA.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Szatanek
authors.forename: Brooke R
authors.initials: BR
authors.lastname: Anderson-White
authors.forename: David M
authors.initials: DM
authors.lastname: Faugno-Fusci
authors.forename: Michael
authors.initials: M
authors.lastname: White
authors.forename: Jeroen P J
authors.initials: JP
authors.lastname: Saeij
authors.forename: Marc-Jan
authors.initials: MJ
authors.lastname: Gubbels
mesh_terms: D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002453:Cell Cycle; D029721:Drosophila Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016193:G1 Phase; D006801:Humans; D008969:Molecular Sequence Data; D017354:Point Mutation; D021381:Protein Transport; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D014122:Toxoplasma / Q000737:chemistry / Q000166:cytology* / Q000235:genetics / Q000378:metabolism*; D014123:Toxoplasmosis / Q000469:parasitology
chemical_list: D002352:Carrier Proteins; D029721:Drosophila Proteins; D015800:Protozoan Proteins; C414093:cactin protein, Drosophila