title: Correlative Light-Ion Microscopy for biological applications.
abstract: Here we report a new technique, Correlative Light-Ion Microscopy (CLIM), to correlate SEM-like micrographs with fluorescence images. This technique presents significant advantages over conventional methods in enabling topographical and biochemical information to be correlated with nanoscale resolution without destroying the fluorescence signal. We demonstrate the utility of CLIM for a variety of investigations of cell substrate interactions validating its potential to become a routine procedure in biomedical research.
authors.affiliation: Department of Materials, Department of Bioengineering and Institute of Biomedical Engineering, Imperial College London, London, SW7 2AZ, UK.
authors.forename: Sergio
authors.initials: S
authors.lastname: Bertazzo
authors.forename: Thomas
authors.initials: T
authors.lastname: von Erlach
authors.forename: Silvia
authors.initials: S
authors.lastname: Goldoni
authors.forename: Pelin L
authors.initials: PL
authors.lastname: Çandarlıoğlu
authors.forename: Molly M
authors.initials: MM
authors.lastname: Stevens