title: Identification of differentially expressed proteins in curcumin-treated prostate cancer cell lines.
abstract: Due to high prevalence and slow progression of prostate cancer, primary prevention appears to be attractive strategy for its eradication. During the last decade, curcumin (diferuloylmethane), a natural compound from the root of turmeric (Curcuma longa), was described as a potent chemopreventive agent. Curcumin exhibits anti-inflammatory, anticarcinogenic, antiproliferative, antiangiogenic, and antioxidant properties in various cancer cell models. This study was designed to identify proteins involved in the anticancer activity of curcumin in androgen-dependent (22Rv1) and -independent (PC-3) human prostate cancer cell lines using two-dimensional difference in gel electrophoresis (2D-DIGE). Out of 425 differentially expressed spots, we describe here the MALDI-TOF-MS analysis of 192 spots of interest, selected by their expression profile. This approach allowed the identification of 60 differentially expressed proteins (32 in 22Rv1 cells and 47 in PC-3 cells). Nineteen proteins are regulated in both cell lines. Further bioinformatic analysis shows that proteins modulated by curcumin are implicated in protein folding (such as heat-shock protein PPP2R1A; RNA splicing proteins RBM17, DDX39; cell death proteins HMGB1 and NPM1; proteins involved in androgen receptor signaling, NPM1 and FKBP4/FKBP52), and that this compound could have an impact on miR-141, miR-152, and miR-183 expression. Taken together, these data support the hypothesis that curcumin is an interesting chemopreventive agent as it modulates the expression of proteins that potentially contribute to prostate carcinogenesis.
authors.affiliation: Laboratoire de Biologie Moléculaire et Cellulaire du Cancer, Hôpital Kirchberg, Luxembourg.
authors.forename: Marie-Hélène
authors.initials: MH
authors.lastname: Teiten
authors.forename: Anthoula
authors.initials: A
authors.lastname: Gaigneaux
authors.forename: Sébastien
authors.initials: S
authors.lastname: Chateauvieux
authors.forename: Anja M
authors.initials: AM
authors.lastname: Billing
authors.forename: Sébastien
authors.initials: S
authors.lastname: Planchon
authors.forename: Fred
authors.initials: F
authors.lastname: Fack
authors.forename: Jenny
authors.initials: J
authors.lastname: Renaut
authors.forename: Fabienne
authors.initials: F
authors.lastname: Mack
authors.forename: Claude P
authors.initials: CP
authors.lastname: Muller
authors.forename: Mario
authors.initials: M
authors.lastname: Dicato
authors.forename: Marc
authors.initials: M
authors.lastname: Diederich
mesh_terms: D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D001483:Base Sequence; D015153:Blotting, Western; D045744:Cell Line, Tumor; D003474:Curcumin / Q000494:pharmacology*; D017931:DNA Primers; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008297:Male; D009363:Neoplasm Proteins / Q000378:metabolism*; D000090243:Nucleophosmin; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D060888:Real-Time Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D016588:Anticarcinogenic Agents; D017931:DNA Primers; C000716967:NPM1 protein, human; D009363:Neoplasm Proteins; D000090243:Nucleophosmin; D003474:Curcumin