title: Oligonucleotide and Parylene Surface Coating of Polystyrene and ePTFE for Improved Endothelial Cell Attachment and Hemocompatibility.
abstract: In vivo self-endothelialization by endothelial cell adhesion on cardiovascular implants is highly desirable. DNA-oligonucleotides are an intriguing coating material with nonimmunogenic characteristics and the feasibility of easy and rapid chemical fabrication. The objective of this study was the creation of cell adhesive DNA-oligonucleotide coatings on vascular implant surfaces. DNA-oligonucleotides immobilized by adsorption on parylene (poly(monoaminomethyl-para-xylene)) coated polystyrene and ePTFE were resistant to high shear stress (9.5 N/m(2)) and human blood serum for up to 96 h. Adhesion of murine endothelial progenitor cells, HUVECs and endothelial cells from human adult saphenous veins as well as viability over a period of 14 days of HUVECs on oligonucleotide coated samples under dynamic culture conditions was significantly enhanced (P < 0.05). Oligonucleotide-coated surfaces revealed low thrombogenicity and excellent hemocompatibility after incubation with human blood. These properties suggest the suitability of immobilization of DNA-oligonucleotides for biofunctionalization of blood vessel substitutes for improved in vivo endothelialization.
authors.affiliation: Department of Thoracic, Cardiac and Vascular Surgery, University Hospital, Hoppe-Seyler-Strasse 3, 72076 Tuebingen, Germany.
authors.forename: Martina
authors.initials: M
authors.lastname: Schleicher
authors.forename: Jan
authors.initials: J
authors.lastname: Hansmann
authors.forename: Bentsian
authors.initials: B
authors.lastname: Elkin
authors.forename: Petra J
authors.initials: PJ
authors.lastname: Kluger
authors.forename: Simone
authors.initials: S
authors.lastname: Liebscher
authors.forename: Agnes J T
authors.initials: AJ
authors.lastname: Huber
authors.forename: Olaf
authors.initials: O
authors.lastname: Fritze
authors.forename: Christine
authors.initials: C
authors.lastname: Schille
authors.forename: Michaela
authors.initials: M
authors.lastname: Müller
authors.forename: Katja
authors.initials: K
authors.lastname: Schenke-Layland
authors.forename: Martina
authors.initials: M
authors.lastname: Seifert
authors.forename: Heike
authors.initials: H
authors.lastname: Walles
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Wendel
authors.forename: Ulrich A
authors.initials: UA
authors.lastname: Stock