title: A crucial role for Ras suppressor-1 (RSU-1) revealed when PINCH and ILK binding is disrupted.
abstract: PINCH, integrin-linked kinase (ILK) and Ras suppressor-1 (RSU-1) are molecular scaffolding proteins that form a physical complex downstream of integrins, and have overlapping roles in cellular adhesion. In Drosophila, PINCH and ILK colocalize in cells and have indistinguishable functions in maintaining wing adhesion and integrin to actin linkage in the muscle. We sought to determine whether the direct physical interaction between PINCH and ILK was essential for their functions using transgenic flies expressing a version of PINCH with a point mutation that disrupts ILK binding (PINCH(Q38A)). We demonstrate that the PINCH-ILK interaction is not required for viability, for integrin-mediated adhesion of the wing or muscle, or for maintaining appropriate localization or levels of either PINCH or ILK. These results suggest alternative modes for PINCH localization, stabilization and linkage to the actin cytoskeleton that are independent of a direct interaction with ILK. Furthermore, we identified a synthetic lethality in flies carrying both the PINCH(Q38A) mutation and a null mutation in the gene encoding RSU-1. This lethality does not result from PINCH mislocalization or destabilization, and illustrates a novel compensatory role for RSU-1 in maintaining viability in flies with compromised PINCH-ILK binding. Taken together, this work highlights the existence of redundant mechanisms in adhesion complex assembly that support integrin function in vivo.
authors.affiliation: Department of Oncological Sciences, University of Utah, Salt Lake City, UT 84112, USA.
authors.forename: Maria C
authors.initials: MC
authors.lastname: Elias
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Pronovost
authors.forename: Kinley J
authors.initials: KJ
authors.lastname: Cahill
authors.forename: Mary C
authors.initials: MC
authors.lastname: Beckerle
authors.forename: Julie L
authors.initials: JL
authors.lastname: Kadrmas
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000196:embryology / Q000235:genetics / Q000378:metabolism; D015153:Blotting, Western; D003433:Crosses, Genetic; D004330:Drosophila / Q000196:embryology* / Q000201:enzymology / Q000235:genetics; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000378:metabolism / Q000473:pathology; D005260:Female; D016023:Integrins / Q000235:genetics / Q000378:metabolism; D046912:Multiprotein Complexes / Q000235:genetics / Q000378:metabolism; D009132:Muscles / Q000166:cytology / Q000378:metabolism; D017354:Point Mutation; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D055550:Protein Stability; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014921:Wings, Animal / Q000166:cytology / Q000378:metabolism; D000099200:Scaffold Protein ILK
chemical_list: D029721:Drosophila Proteins; D016023:Integrins; D046912:Multiprotein Complexes; D017346:Protein Serine-Threonine Kinases; D014157:Transcription Factors; D000099200:Scaffold Protein ILK; C495946:ics protein, Drosophila; C495947:stck protein, Drosophila