title: [Glycomics in quality control of tissue-engineered medical products].
abstract: Glycosylation of cells is known to alter with several biological events such as cell differentiations and proliferations as well as some diseases. "Glycomic approaches", comprehensive qualitative and quantitative glycan analyses of the cells, have become increasingly important as a means of discovering biomarkers that have the potential of being used as disease diagnostic markers and molecular markers for cell characterizations. In this paper, we introduce a method of quantitative glycan profiling by liquid chromatography/mass spectrometry with a combination of an isotope tagging method. In addition, we demonstrate the potential of glycan profiling as a tool for the identification of differentiated human bone marrow mesenchymal stem cell (hMSC) and non-differentiated hMSC.
authors.affiliation: National Institute of Health Sciences, Tokyo, Japan.
authors.forename: Noritaka
authors.initials: N
authors.lastname: Hashii
authors.forename: Shiori
authors.initials: S
authors.lastname: Nakazawa
authors.forename: Nana
authors.initials: N
authors.lastname: Kawasaki
mesh_terms: D000818:Animals; D015415:Biomarkers; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation; D002477:Cells / Q000378:metabolism; D002853:Chromatography, Liquid / Q000379:methods; D017127:Glycation End Products, Advanced; D054794:Glycomics / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D059630:Mesenchymal Stem Cells; D051379:Mice; D011134:Polysaccharides / Q000032:analysis; D011786:Quality Control; D023822:Tissue Engineering
chemical_list: D015415:Biomarkers; D017127:Glycation End Products, Advanced; D011134:Polysaccharides