title: A role of autophagy in Trypanosoma brucei cell death.
abstract: The early branching eukaryote Trypanosoma brucei contains functional autophagy machinery that allows regulated degradation of its own cellular components. In this study, we examined the function of two Atg8 genes, TbAtg8.1 and TbAtg8.2, in starvation-induced autophagosome formation and cell death in procyclic T. brucei. Upon starvation, both TbAtg8.1 and TbAtg8.2 localize to punctate structures characteristic of autophagosomes as shown by fluorescence and electron microscopy, and wortmannin and chloroquine treatments. While TbAtg8.1 depletion has no detectable effects on TbAtg8.2 recruitment to autophagosomes, TbAtg8.2 depletion greatly reduced the autophagosome relocation of TbAtg8.1. Depletion of TbAtg8.1 and 8.2, individually or together, promote cell survival under starvation conditions. Taken together, these observations confirm the presence of an autophagy-related cell death pathway in T. brucei, where TbAtg8.1 and TbAtg8.2 play essential but distinct roles in autophagosome formation and cell death.
authors.affiliation: Department of Biological Sciences Centre for BioImaging Sciences, National University of Singapore, 117543 Singapore. dbslfj@nus.edu.sg
authors.forename: Feng-Jun
authors.initials: FJ
authors.lastname: Li
authors.forename: Qian
authors.initials: Q
authors.lastname: Shen
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Sun
authors.forename: Adam Y
authors.initials: AY
authors.lastname: Yuan
authors.forename: Cynthia Y
authors.initials: CY
authors.lastname: He
mesh_terms: D001343:Autophagy; D055785:Gene Knockdown Techniques; D008856:Microscopy, Fluorescence; D010588:Phagosomes / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D021381:Protein Transport; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D034622:RNA Interference; D013312:Stress, Physiological; D014346:Trypanosoma brucei brucei / Q000235:genetics / Q000502:physiology / Q000648:ultrastructure
chemical_list: D010714:Phosphatidylethanolamines; D015800:Protozoan Proteins; C483858:phosphatidylethanolamine