title: In vitro assessment of relief to oxidative stress by different fractions of Boerhavia procumbens.
abstract: Methanolic extract of Boerhavia procumbens Bank ex Roxb. was partitioned with n-hexane, chloroform, ethyl acetate and n-butanol sequentially after dissolving in distilled water. Phytochemical screening showed presence of phenolics, flavonoides and cardiac glycosides in large amount in chloroform, ethyl acetate and n-butanol soluble fraction. The antioxidant activity of all these fractions and the remaining aqueous fraction was evaluated by four methods such as: 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, ferric reducing antioxidant power (FRAP) assay, total antioxidant activity and ferric thiocyanate assay. Total phenolics were also determined. Some fractions showed noteworthy antioxidant activity. The results of the antioxidant activity revealed that the ethyl acetate soluble fraction showed the highest value of percent inhibition of DPPH (82.54 ± 0.62) at the concentration of 125 μ g/ml. The IC(50) of this fraction was 37.11± 0.23 μg/ml, compared with butylated hydroxytoluene (BHT), which have IC(50) of 12.1 ± 0.92 μ/mL. It also showed the highest FRAP value (251.08 ± 1.46 μg of trolox equivalents) as well as the highest value of lipid peroxidation inhibition (57.21 ± 52%), the highest total antioxidant activity (0.549 ± 0.08) and also the highest total phenolic contents (77.1 ± 0.6) as compared to the studied fractions. Phytochemical screening showed high percentage of phenolics, flavonoides and cardiac glycosides in this fraction.
authors.affiliation: Department of Chemistry, Government College University, Lahore, Pakistan. atrabbasi@yahoo.com
authors.forename: Muhammad Athar
authors.initials: MA
authors.lastname: Abbasi
authors.forename: Kaniz
authors.initials: K
authors.lastname: Rubab
authors.forename: Azizur
authors.initials: A
authors.lastname: Rehman
authors.forename: Tauheeda
authors.initials: T
authors.lastname: Riaz
authors.forename: Tayyaba
authors.initials: T
authors.lastname: Shahzadi
authors.forename: Muniba
authors.initials: M
authors.lastname: Khalid
authors.forename: Muhammad
authors.initials: M
authors.lastname: Ajaib
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D039281:Nyctaginaceae / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects*; D010636:Phenols / Q000032:analysis; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D010636:Phenols; D010936:Plant Extracts