title: Effects of a combined treatment with GPR30 agonist G-1 and herceptin on growth and gene expression of human breast cancer cell lines.
abstract: Expression of G-protein-coupled receptor 30 (GPR30) is present in HER2-overexpressing breast cancer. In this study, we examined to what extent GPR30-agonist G-1 would affect the antitumoral action of trastuzumab (Herceptin). Combined treatment with both drugs exerted an additive growth-inhibitory effect on breast cancer cells which was accompanied by a significant decline of cyclin A2 expression both on the protein and the mRNA level. Combined treatment also resulted in expression changes of c-fos, cyclin D1, or p21/WAF-1. The results of our study encourage further attempts to test the relevance of these in vitro data in the clinical setting.
authors.affiliation: Department of Obstetrics and Gynecology, University Medical Center Regensburg,   Regensburg,   Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Lubig
authors.forename: Claus
authors.initials: C
authors.lastname: Lattrich
authors.forename: Anette
authors.initials: A
authors.lastname: Springwald
authors.forename: Julia
authors.initials: J
authors.lastname: Häring
authors.forename: Susanne
authors.initials: S
authors.lastname: Schüler
authors.forename: Olaf
authors.initials: O
authors.lastname: Ortmann
authors.forename: Oliver
authors.initials: O
authors.lastname: Treeck
mesh_terms: D061067:Antibodies, Monoclonal, Humanized / Q000008:administration & dosage / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003517:Cyclopentanes / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D016762:Genes, fos; D006801:Humans; D011804:Quinolines / Q000494:pharmacology*; D011960:Receptors, Estrogen / Q000819:agonists*; D043562:Receptors, G-Protein-Coupled / Q000819:agonists*; D000068878:Trastuzumab
chemical_list: C517943:1-(4-(6-bromobenzo(1,3)dioxol-5-yl)-3a,4,5,9b-tetrahydro-3H-cyclopenta(c)quinolin-8-yl)ethanone; D061067:Antibodies, Monoclonal, Humanized; D003517:Cyclopentanes; C109584:GPER1 protein, human; D011804:Quinolines; D011960:Receptors, Estrogen; D043562:Receptors, G-Protein-Coupled; D000068878:Trastuzumab