title: Salmonella infects B cells by macropinocytosis and formation of spacious phagosomes but does not induce pyroptosis in favor of its survival.
abstract: We have previously reported that Salmonella infects B cells and survives within endosomal-lysosomal compartments. However, the mechanisms used by Salmonella to enter B cells remain unknown. In this study, we have shown that Salmonella induces its own entry by the induction of localized ruffling, macropinocytosis, and spacious phagosome formation. These events were associated with the rearrangement of actin and microtubule networks. The Salmonella pathogenesis island 1 (SPI-1) was necessary to invade B cells. In contrast to macrophages, B cells were highly resistant to cell death induced by Salmonella. These data demonstrate the ability of Salmonella to infect these non-professional phagocytic cells, where the bacterium can find an ideal intracellular niche to support persistence and the possible dissemination of infection.
authors.affiliation: Laboratorio de Infectología, Microbiología e Inmunología Clínicas, Departamento de Medicina Experimental, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico.
authors.forename: Roberto
authors.initials: R
authors.lastname: Rosales-Reyes
authors.forename: Araceli
authors.initials: A
authors.lastname: Pérez-López
authors.forename: Concepción
authors.initials: C
authors.lastname: Sánchez-Gómez
authors.forename: Rosaura Ruth
authors.initials: RR
authors.lastname: Hernández-Mote
authors.forename: Denisse
authors.initials: D
authors.lastname: Castro-Eguiluz
authors.forename: Vianney
authors.initials: V
authors.lastname: Ortiz-Navarrete
authors.forename: Celia Mercedes
authors.initials: CM
authors.lastname: Alpuche-Aranda
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D001402:B-Lymphocytes / Q000382:microbiology* / Q000502:physiology*; D001426:Bacterial Proteins; D002478:Cells, Cultured; D005260:Female; D054884:Host-Pathogen Interactions; D008264:Macrophages / Q000382:microbiology / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008870:Microtubules / Q000378:metabolism; D010588:Phagosomes / Q000382:microbiology*; D010873:Pinocytosis; D012475:Salmonella / Q000235:genetics / Q000472:pathogenicity*; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D000199:Actins; D001426:Bacterial Proteins; C419942:Spi1 protein, Salmonella; D037521:Virulence Factors