title: Effective expansion of human adipose-derived stromal cells and bone marrow-derived mesenchymal stem cells cultured on a fragmin/protamine nanoparticles-coated substratum with human platelet-rich plasma.
abstract: Fragmin/protamine nanoparticles (F/P NPs) can be stably coated onto plastic surfaces and used as a substratum for the absorption and controlled release of growth factors (GFs) secreted from human platelet-rich plasma (PRP). In this study, we investigated the capability of F/P NP-coated plates to act as a substratum for the proliferation of human adipose-derived stromal cells (ASCs) and bone marrow-derived mesenchymal stem cells (BMSCs) with GFs in PRP. Both cell types adhered well to the F/P NP-coated plates and grew optimally, with a doubling time of 30 and 32 h in low-concentration PRP (0.5%) medium supplemented with 5 ng/ml fibroblast growth factor-2 (FGF-2) on the F/P NP-coated plates. These cells maintained their multilineage potential for differentiation into adipocytes or osteoblasts. Furthermore, ASCs and BMSCs grew well in medium without PRP and FGF-2 on F/P NP-coated plates pretreated with PRP and FGF-2 in a concentration-dependent manner. Thus, F/P NP-coated plates are a useful substratum for the adherence and proliferation of ASCs and BMSCs in low-concentration PRP medium supplemented with FGF-2. No xenogeneic serum is required.
authors.affiliation: Research Institute, National Defence Medical College, Saitama, Japan; Reseach Fellow of the Japan Society for the Promotion of Science, Tokyo, Japan; Department of Biosciences and Informatics, School of Fundamental Science and Technology, Keio University, Yokohama, Japan.
authors.forename: Satoko
authors.initials: S
authors.lastname: Kishimoto
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ishihara
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Mori
authors.forename: Megumi
authors.initials: M
authors.lastname: Takikawa
authors.forename: Hidemi
authors.initials: H
authors.lastname: Hattori
authors.forename: Shingo
authors.initials: S
authors.lastname: Nakamura
authors.forename: Toshinori
authors.initials: T
authors.lastname: Sato
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D001854:Bone Marrow Cells / Q000166:cytology*; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D017985:Dalteparin / Q000494:pharmacology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D053657:Platelet-Rich Plasma / Q000378:metabolism*; D011479:Protamines / Q000494:pharmacology*; D046509:Tissue Culture Techniques
chemical_list: D011479:Protamines; D017985:Dalteparin