title: Interaction of cisplatin with human superoxide dismutase.
abstract: cis-Diamminedichloroplatinum(II) (cisplatin) is able to interact with human superoxide dismutase (hSOD1) in the disulfide oxidized apo form with a dissociation constant of 37 ± 3 μM through binding cysteine 111 (Cys111) located at the edge of the subunit interface. It also binds to Cu(2)-Zn(2) and Zn(2)-Zn(2) forms of hSOD1. Cisplatin inhibits aggregation of demetalated oxidized hSOD1, and it is further able to dissolve and monomerize oxidized hSOD1 oligomers in vitro and in cell, thus indicating its potential as a leading compound for amyotrophic lateral sclerosis.
authors.affiliation: Magnetic Resonance Center CERM, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Florence, Italy. banci@cerm.unifi.it
authors.forename: Lucia
authors.initials: L
authors.lastname: Banci
authors.forename: Ivano
authors.initials: I
authors.lastname: Bertini
authors.forename: Olga
authors.initials: O
authors.lastname: Blaževitš
authors.forename: Vito
authors.initials: V
authors.lastname: Calderone
authors.forename: Francesca
authors.initials: F
authors.lastname: Cantini
authors.forename: Jiafei
authors.initials: J
authors.lastname: Mao
authors.forename: Angela
authors.initials: A
authors.lastname: Trapananti
authors.forename: Miguela
authors.initials: M
authors.lastname: Vieru
authors.forename: Ilaria
authors.initials: I
authors.lastname: Amori
authors.forename: Mauro
authors.initials: M
authors.lastname: Cozzolino
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Carrì
mesh_terms: D000818:Animals; D002460:Cell Line; D002945:Cisplatin / Q000737:chemistry / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D008958:Models, Molecular; D012996:Solutions; D013329:Structure-Activity Relationship; D013482:Superoxide Dismutase / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism
chemical_list: D004791:Enzyme Inhibitors; D012996:Solutions; D013482:Superoxide Dismutase; D002945:Cisplatin