title: Soymilk: an effective and inexpensive blocking agent for immunoblotting.
abstract: Blocking efficacy of whole soymilk, nonfat soymilk, SuperBlock, and nonfat milk was evaluated by performing standard protein immunoblotting procedures on both purified protein and crude nuclear extracts from HEK 293 cells. Nonfat soymilk was found to have superior blocking efficacy compared with other blocking agents in terms of high signal-to-noise ratio with the shortest blocking times. In addition, the presence of low concentrations of the detergent Tween 20 (0.05-0.1%, v/v) in the wash buffer as well as antibody incubations significantly lessened the background compared with including only the detergent during wash steps.
authors.affiliation: School of Biological Sciences, Illinois State University, Normal, IL 61790, USA.
authors.forename: Charitha
authors.initials: C
authors.lastname: Galva
authors.forename: Craig
authors.initials: C
authors.lastname: Gatto
authors.forename: Mark
authors.initials: M
authors.lastname: Milanick
mesh_terms: D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D057809:HEK293 Cells; D006801:Humans; D011136:Polysorbates / Q000737:chemistry; D041942:Soy Milk / Q000737:chemistry*
chemical_list: D011136:Polysorbates