title: Binding interaction of 1-(4-methybenzyl)-2-p-tolyl-1H-benzo[d]imidazole with bovine serum albumin.
abstract: A promising benzimidazole derivative 1-(4-methybenzyl)-2-p-tolyl-1H-benzo[d]imidazole (MBTBI) has been synthesized and characterized by single crystal XRD, NMR, mass and IR spectral techniques. The mutual interaction of this benzimidazole derivative (MBTBI) with bovine serum albumin (BSA) was investigated using solution spectral studies. The fluorescence quenching mechanism of BSA by MBTBI was analyzed and the binding constant has been calculated. The binding distance between these two was obtained based on the theory of Forester's non-radiation energy transfer (FRET). The effect of some common ions on the binding constant was also examined.
authors.affiliation: Department of Chemistry, Annamalai University, Annamalainagar, Tamilnadu 608002, India. jtchalam2005@yahoo.co.in
authors.forename: J
authors.initials: J
authors.lastname: Jayabharathi
authors.forename: V
authors.initials: V
authors.lastname: Thanikachalam
authors.forename: K
authors.initials: K
authors.lastname: Jayamoorthy
authors.forename: N
authors.initials: N
authors.lastname: Srinivasan
mesh_terms: D000818:Animals; D001562:Benzimidazoles / Q000737:chemistry / Q000378:metabolism*; D002417:Cattle; D018360:Crystallography, X-Ray; D031541:Fluorescence Resonance Energy Transfer; D019906:Nuclear Magnetic Resonance, Biomolecular; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013055:Spectrophotometry, Infrared
chemical_list: D001562:Benzimidazoles; D012710:Serum Albumin, Bovine