title: Investigation of genome instability in exfoliated colonic epithelial cells and in mitogen-stimulated lymphocytes of patients with ulcerative colitis.
abstract: OBJECTIVE
  The present study aimed to evaluate the micronucleus (MN), nucleoplasmic bridges (NPBs) and nuclear buds (NBUDs) in the mitogen-stimulated lymphocytes of patients with ulcerative colitis (UC). In addition, we assessed MN frequency in exfoliated colonic epithelial cells obtained from both the diseased and healthy colonic mucosa of patients.
  
  
  METHODS
  The study was conducted in 22 newly diagnosed patients with UC and in 22 healthy controls. MN, NPB and NBUD values scored in binucleated (BN) cells were obtained from the mitogen-stimulated lymphocytes of patients and control subjects. In addition, the MN values in exfoliated epithelial cells obtained from the diseased and healthy colonic mucosa of patients were evaluated.
  
  
  RESULTS
  We found significantly higher MN, NPB and NBUD frequencies in the BN cells of patients with UC than in those of the control subjects (1.61 ± 0.75 vs. 0.89 ± 0.29, 3.93 ± 1.91 vs. 1.39 ± 1.10, and 1.55 ± 0.89 vs. 0.64 ± 0.48, p = 0.001). Also, a statistically significant difference was found between MN frequencies obtained from the diseased and healthy colonic mucosa of patients (1.07 ± 0.46 vs. 0.59 ± 0.21, p = 0.001). No significant relationship was found between age and MN frequency in patients with UC (r = 0.076, p = 0.735).
  
  
  CONCLUSIONS
  Increased MN, NPB and NBUD frequencies observed in both the lymphocytes and exfoliated colonic epithelial cells obtained from patients with UC may reflect genomic instability.
authors.affiliation: Department of Gastroenterology, Erciyes University, Kayseri, Turkey. drkaraman@hotmail.com
authors.forename: Ahmet
authors.initials: A
authors.lastname: Karaman
authors.forename: Zuhal
authors.initials: Z
authors.lastname: Hamurcu
authors.forename: Hamiyet
authors.initials: H
authors.lastname: Donmez
authors.forename: Nazmiye
authors.initials: N
authors.lastname: Bitken
authors.forename: Hatice
authors.initials: H
authors.lastname: Karaman
authors.forename: Mevlut
authors.initials: M
authors.lastname: Baskol
authors.forename: Sebnem
authors.initials: S
authors.lastname: Gursoy
authors.forename: Kadri
authors.initials: K
authors.lastname: Guven
authors.forename: Omer
authors.initials: O
authors.lastname: Ozbakir
authors.forename: Mehmet
authors.initials: M
authors.lastname: Yucesoy
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D003093:Colitis, Ulcerative / Q000473:pathology*; D003106:Colon / Q000473:pathology*; D004847:Epithelial Cells / Q000648:ultrastructure*; D005260:Female; D042822:Genomic Instability; D006801:Humans; D007413:Intestinal Mucosa / Q000473:pathology*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000648:ultrastructure*; D008297:Male; D015162:Micronucleus Tests; D008875:Middle Aged; D055815:Young Adult