title: STING mediates neuronal innate immune response following Japanese encephalitis virus infection.
abstract: Flavivirus-mediated inflammation causes neuronal death, but whether the infected neurons can evoke an innate immune response to elicit their own protection, is unknown. In an earlier study we have shown that neuronal RIG-I, play a significant role in inducing production and release of molecules that are related to inflammation. In this study, using a neuronal cell line, we show that RIG-I acts with STING in a concerted manner following its interaction with Japanese encephalitis viral RNA to induce a type 1 interferon response. Knock-down of STING showed that the expressions of various inflammatory signaling molecules were down-regulated along with increased intracellular viral load. Alternatively, over-expressing STING decreased intracellular viral load. Our results indicate that at the sub-cellular level, interaction between the pattern recognition receptor RIG-I and the adapter molecule STING, is a major contributor to elicit immunological responses involving the type 1 interferons in neurons following JEV infections.
authors.affiliation: National Brain Research Centre , Manesar, Haryana-122051, India.
authors.forename: Arshed
authors.initials: A
authors.lastname: Nazmi
authors.forename: Rupanjan
authors.initials: R
authors.lastname: Mukhopadhyay
authors.forename: Kallol
authors.initials: K
authors.lastname: Dutta
authors.forename: Anirban
authors.initials: A
authors.lastname: Basu
mesh_terms: D051546:Cyclooxygenase 2 / Q000378:metabolism; D004664:Encephalitis Virus, Japanese / Q000235:genetics; D004672:Encephalitis, Japanese / Q000276:immunology*; D006801:Humans; D007113:Immunity, Innate / Q000502:physiology*; D050838:Interferon Regulatory Factor-3 / Q000378:metabolism; D050839:Interferon Regulatory Factor-7 / Q000378:metabolism; D008565:Membrane Proteins / Q000502:physiology*; D016328:NF-kappa B / Q000378:metabolism; D009474:Neurons / Q000276:immunology*; D015854:Up-Regulation; D019562:Viral Load; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism; D000099197:STING Protein
chemical_list: D051546:Cyclooxygenase 2; D050838:Interferon Regulatory Factor-3; D050839:Interferon Regulatory Factor-7; D008565:Membrane Proteins; D016328:NF-kappa B; D048051:p38 Mitogen-Activated Protein Kinases; C494232:IRF3 protein, human; C494234:IRF7 protein, human; C532857:STING1 protein, human; D000099197:STING Protein