title: CYP1A1 is a target of miR-892a-mediated post-transcriptional repression.
abstract: Cytochrome P450 1A1 (CYP1A1) is a member of the cytochrome p450 enzyme family,             which is involved in the metabolisms of carcinogenic metabolites, such as benzo(a)pyrene.             In this study, we identified miR-892a as a negative regulator of CYP1A1 expression.             Luciferase assays revealed a sequence in the 3'-untranslated region of CYP1A1             that displayed a perfect match with miR-892a, and revealed that this sequence             was a specific miR-892a target site. The overexpression of miR‑892a inhibited             the expression of the CYP1A1 protein, and the miR‑892a antagonist increased CYP1A1             expression. Of note, benzo(a)pyrene, a major inducer of CYP1A1 transcription,             decreased the expression of miR-892a. Moreover, the miR-892a-induced CYP1A1 repression             inhibited the benzo(a)pyrene-mediated decrease in cell viability. These data provide             insight into the CYP1A1 regulatory network.
authors.affiliation: Functional Genoproteome Research Centre and LIFEnGENE Inc, Seoul 143-701, Republic of Korea.
authors.forename: Yeong Min
authors.initials: YM
authors.lastname: Choi
authors.forename: Sungkwan
authors.initials: S
authors.lastname: An
authors.forename: Eun-Mee
authors.initials: EM
authors.lastname: Lee
authors.forename: Karam
authors.initials: K
authors.lastname: Kim
authors.forename: Sung Jin
authors.initials: SJ
authors.lastname: Choi
authors.forename: Ju Sub
authors.initials: JS
authors.lastname: Kim
authors.forename: Hyun Hee
authors.initials: HH
authors.lastname: Jang
authors.forename: In-Sook
authors.initials: IS
authors.lastname: An
authors.forename: Seunghee
authors.initials: S
authors.lastname: Bae
mesh_terms: D020413:3' Untranslated Regions; D020029:Base Pairing; D001483:Base Sequence; D001564:Benzo(a)pyrene / Q000494:pharmacology; D002273:Carcinogens / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D006801:Humans; D049409:Luciferases, Firefly / Q000096:biosynthesis / Q000235:genetics; D035683:MicroRNAs / Q000378:metabolism / Q000502:physiology*; D008969:Molecular Sequence Data; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D015533:Transcriptional Activation / Q000187:drug effects
chemical_list: D020413:3' Untranslated Regions; D002273:Carcinogens; C574666:MIRN892 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D001564:Benzo(a)pyrene; D049409:Luciferases, Firefly; C569463:CYP1A1 protein, human; D019363:Cytochrome P-450 CYP1A1