title: Cytokine release from human leukocytes exposed to silorane- and methacrylate-based dental materials.
abstract: OBJECTIVE
  Silorane-based dental monomers contain an epoxy functional group. Less is known about the toxicological and inflammatory potential of silorane-based composites. Therefore we compared the release of 24 cytokines from human leukocytes after incubation with silorane-based Filtek™ Silorane (Silo) and methacrylate-based TetricEvo Flow® (TC).
  
  
  METHODS
  Leukocytes from nine healthy test persons (P) were incubated with Silo or TC for up to 72h. All 24h cytokines were quantified with a magnetic bead assay.
  
  
  RESULTS
  Silo stimulates the leukocytes to higher release of cytokines when compared to TC. 72h after beginning the experiment, leukocytes from P6 incubated with Silo secreted more than an 18-fold amount of interleukin (IL)-6 when compared with leukocytes incubated with TC (771.8 vs 42.1pg/ml). Only leukocytes from P8 incubated with Silo release up to 14.4pg/ml IL-2 after 72h.
  
  
  CONCLUSIONS
  The significantly higher induction of cytokines with Silo in comparison to TC is test person independent. This indicates a higher sensitization potential for Silo. Because of the cytokine release pattern (especially the release of T-cell dependent IL-2) from leukocytes from P8 after incubation with Silo it is likely that P8 can develop an allergic Type IV sensitization to Silo. Therefore the cytokine release assay is a helpful tool for providing information about possible immunological reactions to dental resins in individual cases as well as for a general risk assessment and comparison between different dental materials.
authors.affiliation: Department of Operative/Restorative Dentistry, Periodontology and Pedodontics, Ludwig-Maximilians-University of Munich, Goethestrasse 70, Munich, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Wellner
authors.forename: W
authors.initials: W
authors.lastname: Mayer
authors.forename: R
authors.initials: R
authors.lastname: Hickel
authors.forename: F X
authors.initials: FX
authors.lastname: Reichl
authors.forename: J
authors.initials: J
authors.lastname: Durner
mesh_terms: D016207:Cytokines / Q000096:biosynthesis*; D004342:Drug Hypersensitivity / Q000276:immunology*; D006801:Humans; D007249:Inflammation / Q000276:immunology; D007376:Interleukin-2 / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D007962:Leukocytes / Q000187:drug effects* / Q000378:metabolism; D008689:Methacrylates / Q000009:adverse effects / Q000494:pharmacology*; D012833:Siloxanes / Q000009:adverse effects / Q000494:pharmacology*
chemical_list: D016207:Cytokines; D007376:Interleukin-2; D015850:Interleukin-6; D008689:Methacrylates; D012833:Siloxanes