title: Gastroprotective activities of a polysaccharide from the fruiting bodies of Pleurotus ostreatus in rats.
abstract: In this study, a water-soluble polysaccharide (POPw) was successfully purified and identified from the fruiting bodies of Pleurotus ostreatus, with a molecular weight of 2.3×10(4)Da. POPw contained 97.1% total sugar, 0.3% uronic acid and 0.2% protein. Gas chromatography (GC) analysis suggested that POPw was composed of Glc (52.3%), Gal (25.8%), Man (10.0%), Rha (6.1%), and Ara (5.2%). Animal experiments showed that oral administration with POPw significantly inhibited acetic acid-induced gastric lesions in rats, accompanied with a significant increase in mucus synthesis and the prostaglandin production. In addition, POPw could significantly increase the level of glutathione (GSH) and the activity of superoxide dismutase (SOD), as well as decreasing the content of thiobarbituric acid-reactive substances (TBARS) in acetic acid-treated rats with gastric ulcer. These results suggested that the gastroprotective effects of POPw on mice ulcer models can be attributed to its ameliorating effect on oxidative damage and reinforcing effect of gastric mucosal resistance.
authors.affiliation: Department of Gastroenterology, Tangdu Hospital of the Forth Military Medical University, Xi'an 710038, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Yang
authors.forename: Bo
authors.initials: B
authors.lastname: Huang
authors.forename: Huiyan
authors.initials: H
authors.lastname: Li
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.forename: Rong
authors.initials: R
authors.lastname: Zhang
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Huang
authors.forename: Jingjie
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D000897:Anti-Ulcer Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology; D048690:Fruiting Bodies, Fungal / Q000737:chemistry*; D005978:Glutathione / Q000378:metabolism; D008297:Male; D008970:Molecular Weight; D009093:Mucus / Q000378:metabolism; D020076:Pleurotus / Q000737:chemistry*; D011134:Polysaccharides / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D011453:Prostaglandins / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000897:Anti-Ulcer Agents; D000975:Antioxidants; D011134:Polysaccharides; D011453:Prostaglandins; D017392:Thiobarbituric Acid Reactive Substances; D013482:Superoxide Dismutase; D005978:Glutathione