title: Analysis of the physical activity effects and measurement of pro-inflammatory cytokines in irradiated lungs in rats.
abstract: OBJECTIVE
  To study if the pre-radiotherapy physical activity has radio-protective elements, by measuring the radio-induced activation of pro-inflammatory cytokines as interleukin-6 (il-6), transforming growth factor -β (tgf -β), tumor necrosis factor -α (tnf-α) and protein beta kinase β (ikkβ), through western blotting analysis.
  
  
  METHODS
  A randomized study with 28 Wistar hannover rats, males, with a mean age of 90 days and weighing about 200 grams. The animals were divided into three groups: (GI, GII and GIII). GIII group were submitted to swimming for eight weeks (zero load, three times a week, about 30 minutes). Then, the groups (except the control group) were submitted to irradiation by cobalt therapy, single dose of 3.5 gray in the whole body. All animals were sacrificed by overdose of pentobarbital, according to the time for analysis of cytokines, and then a fragment of the lower lobe of the right lung went to western blotting analysis.
  
  
  RESULTS
  The cytokines IKK β, TNF-α and IL-6 induced by radiation in the lung were lower in the exercised animals. However, exercise did not alter the radiation-induced increase in tgf-β.
  
  
  CONCLUSIONS
  The results show a lower response in relation to inflammatory cytokines in the group that practiced the exercise pre-radiotherapy, showing that exercise can protect tissues from tissue damage due to irradiation.
authors.affiliation: Faculty of Medical Sciences, UNICAMP, Campinas-SP, Brazil. renatagennari@gmail.com
authors.forename: Renata Cristiane Gennari
authors.initials: RC
authors.lastname: Bianchi
authors.forename: Eduardo Rochete
authors.initials: ER
authors.lastname: Ropelle
authors.forename: Carlos Kiyoshi
authors.initials: CK
authors.lastname: Katashima
authors.forename: José Barreto Campello
authors.initials: JB
authors.lastname: Carvalheira
authors.forename: Luiz Roberto
authors.initials: LR
authors.lastname: Lopes
authors.forename: Nelson Adami
authors.initials: NA
authors.lastname: Andreollo
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western; D003037:Cobalt Radioisotopes / Q000008:administration & dosage; D051550:I-kappa B Kinase / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism*; D008168:Lung / Q000378:metabolism / Q000528:radiation effects*; D008297:Male; D010805:Physical Conditioning, Animal / Q000502:physiology*; D011833:Radiation Injuries, Experimental / Q000139:chemically induced / Q000378:metabolism* / Q000517:prevention & control; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D003037:Cobalt Radioisotopes; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D051550:I-kappa B Kinase