title: Gene expression signature of DMBA-induced hamster buccal pouch carcinomas: modulation by chlorophyllin and ellagic acid.
abstract: Chlorophyllin (CHL), a water-soluble, semi-synthetic derivative of chlorophyll and ellagic acid (EA), a naturally occurring polyphenolic compound in berries, grapes, and nuts have been reported to exert anticancer effects in various human cancer cell lines and in animal tumour models. The present study was undertaken to examine the mechanism underlying chemoprevention and changes in gene expression pattern induced by dietary supplementation of chlorophyllin and ellagic acid in the 7,12-dimethylbenz[a]anthracene (DMBA)-induced hamster buccal pouch (HBP) carcinogenesis model by whole genome profiling using pangenomic microarrays. In hamsters painted with DMBA, the expression of 1,700 genes was found to be altered significantly relative to control. Dietary supplementation of chlorophyllin and ellagic acid modulated the expression profiles of 104 and 37 genes respectively. Microarray analysis also revealed changes in the expression of TGFβ receptors, NF-κB, cyclin D1, and matrix metalloproteinases (MMPs) that may play a crucial role in the transformation of the normal buccal pouch to a malignant phenotype. This gene expression signature was altered on treatment with chlorophyllin and ellagic acid. Our study has also revealed patterns of gene expression signature specific for chlorophyllin and ellagic acid exposure. Thus dietary chlorophyllin and ellagic acid that can reverse gene expression signature associated with carcinogenesis are novel candidates for cancer prevention and therapy.
authors.affiliation: Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Tamil Nadu, India.
authors.forename: Ramamurthi
authors.initials: R
authors.lastname: Vidya Priyadarsini
authors.forename: Neeraj
authors.initials: N
authors.lastname: Kumar
authors.forename: Imran
authors.initials: I
authors.lastname: Khan
authors.forename: Paranthaman
authors.initials: P
authors.lastname: Thiyagarajan
authors.forename: Paturu
authors.initials: P
authors.lastname: Kondaiah
authors.forename: Siddavaram
authors.initials: S
authors.lastname: Nagini
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D002294:Carcinoma, Squamous Cell / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology / Q000517:prevention & control; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000235:genetics; D002735:Chlorophyllides / Q000494:pharmacology* / Q000627:therapeutic use; D006224:Cricetinae; D019587:Dietary Supplements; D004610:Ellagic Acid / Q000494:pharmacology* / Q000627:therapeutic use; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D008297:Male; D008647:Mesocricetus; D009061:Mouth Mucosa / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D009062:Mouth Neoplasms / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology / Q000517:prevention & control; D014158:Transcription, Genetic; D047368:Tumor Burden
chemical_list: D016588:Anticarcinogenic Agents; D002735:Chlorophyllides; D004610:Ellagic Acid; D015127:9,10-Dimethyl-1,2-benzanthracene; C007020:chlorophyllin