title: Investigations on photoinduced interaction of 9-aminoacridine with certain catechols and rutin.
abstract: The fluorescence quenching of 9-aminoacridine by certain biologically important catechols and rutin was investigated using absorption, steady state and time resolved fluorescence measurements. The in vitro-antioxidant activities of the above compounds were studied using deoxyribose degradation assay and nitric oxide scavenging assay. The experimental results showed that the fluorescence of 9-aminoacridine was quenched by quencher molecules via forming ground state complex. The bimolecular quenching rate constant k(q), binding constant (K) and number of binding sites (n) were calculated at different temperatures from relevant fluorescence data. Static quenching mechanism was supported by lifetime measurement. The free energy change (ΔG(et)) for electron transfer process was calculated by Rehm-Weller equation. The binding distance of 4-nitrocatechol with 9-aminoacridine was obtained according to Forster's non-radiative energy transfer theory. Nature of binding forces and their interactions was probed based on thermodynamic parameters.
authors.affiliation: School of Chemistry, Bharathidasan University, Tiruchirappalli-620 024, Tamil Nadu, India.
authors.forename: C
authors.initials: C
authors.lastname: Manivannan
authors.forename: K Meenakshi
authors.initials: KM
authors.lastname: Sundaram
authors.forename: R
authors.initials: R
authors.lastname: Renganathan
authors.forename: M
authors.initials: M
authors.lastname: Sundararaman
mesh_terms: D000585:Aminacrine / Q000737:chemistry*; D001665:Binding Sites; D002396:Catechols / Q000737:chemistry*; D003855:Deoxyribose / Q000737:chemistry; D004298:Dopamine / Q000737:chemistry; D004563:Electrochemistry; D004735:Energy Transfer; D016166:Free Radical Scavengers / Q000737:chemistry*; D007980:Levodopa / Q000737:chemistry; D009569:Nitric Oxide / Q000737:chemistry; D010084:Oxidation-Reduction; D055668:Photochemical Processes; D012431:Rutin / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013050:Spectrometry, Fluorescence
chemical_list: D002396:Catechols; D016166:Free Radical Scavengers; D012997:Solvents; D009569:Nitric Oxide; D007980:Levodopa; D003855:Deoxyribose; D012431:Rutin; D000585:Aminacrine; C034221:catechol; D004298:Dopamine