title: In vitro transfection with plasmid DNA using polylysine copolymers.
abstract: The terplex system for delivering DNA to targeted cells comprises three components-stearyl polylysine (PLL), low-density lipoprotein (LDL), and DNA. This article describes a method for optimizing the relative amounts of these components and determining the transfection parameters for a given cell line.
authors.forename: Sung Wan
authors.initials: SW
authors.lastname: Kim
mesh_terms: D000818:Animals; D002460:Cell Line; D004247:DNA / Q000378:metabolism*; D057927:Hydrophobic and Hydrophilic Interactions; D008077:Lipoproteins, LDL / Q000737:chemistry* / Q000378:metabolism*; D051379:Mice; D010957:Plasmids / Q000378:metabolism; D011107:Polylysine / Q000737:chemistry* / Q000378:metabolism*; D014162:Transfection / Q000379:methods*
chemical_list: D008077:Lipoproteins, LDL; D011107:Polylysine; D004247:DNA