title: Inhibition of melanogenesis versus antioxidant properties of essential oil extracted from leaves of Vitex negundo Linn and chemical composition analysis by GC-MS.
abstract: This study was aimed at investigating the antimelanogenic and antioxidative properties of the essential oil extracted from leaves of V. negundo Linn and the analysis of the chemical composition of this essential oil. The efficacy of the essential oil was evaluated spectrophotometrically, whereas the volatile chemical compounds in the essential oil were analyzed by gas chromatography-mass spectrometry (GC-MS). The results revealed that the essential oil effectively suppresses murine B16F10 tyrosinase activity and decreases the amount of melanin in a dose-dependent manner. Additionally, the essential oil significantly scavenged 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radicals, and showed potent reducing power versus metal-ion chelating properties in a dose-dependent pattern. The chemical constituents in the essential oil are sesquiterpenes (44.41%), monoterpenes (19.25%), esters (14.77%), alcohols (8.53%), aromatic compound (5.90%), ketone (4.96%), ethers (0.4%) that together account for 98.22% of its chemical composition. It is predicted that the aromatic compound in the essential oil may contribute to its antioxidant activities. The results indicated that essential oil extracted from V. negundo Linn leaves decreased melanin production in B16F10 melanoma cells and showed potent antioxidant activities. The essential oil can thereby serve as an inhibitor of melanin synthesis and could also act as a natural antioxidant.
authors.affiliation: Department of Medical Laboratory Science and Biotechnology, China Medical University, No. 91 Hsueh-Shih Road, Taichung City 40402, Taiwan. lchuang@mail.cmu.edu.tw
authors.forename: Huey-Chun
authors.initials: HC
authors.lastname: Huang
authors.forename: Tzu-Yun
authors.initials: TY
authors.lastname: Chang
authors.forename: Long-Zen
authors.initials: LZ
authors.lastname: Chang
authors.forename: Hsiao-Fen
authors.initials: HF
authors.lastname: Wang
authors.forename: Kuang-Hway
authors.initials: KH
authors.lastname: Yih
authors.forename: Wan-Yu
authors.initials: WY
authors.lastname: Hsieh
authors.forename: Tsong-Min
authors.initials: TM
authors.lastname: Chang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology*; D002614:Chelating Agents / Q000737:chemistry / Q000494:pharmacology; D008401:Gas Chromatography-Mass Spectrometry; D007477:Ions; D008543:Melanins / Q000096:biosynthesis*; D008546:Melanoma, Experimental / Q000378:metabolism; D008670:Metals; D051379:Mice; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors; D009822:Oils, Volatile / Q000737:chemistry* / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D020950:Vitex / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D002614:Chelating Agents; D007477:Ions; D008543:Melanins; D008670:Metals; D009822:Oils, Volatile; D014442:Monophenol Monooxygenase