title: Gualou xiebai banxia decoction inhibits NF-kappa B-dependent inflammation in myocardial ischemia-reperfusion injury in rats.
abstract: OBJECTIVE
  To evaluate the myocardial protective effect of Gualou Xiebai Banxia decoction GXBD) and explore the mechanisms of inhibition of NF-kappa B activation and blockade of inflammatory responses induced by ischemia-reperfusion in rats.
  
  
  METHODS
  Twenty-four Sprague Dawley (SD) rats were randomly divided into three groups. Rats in the treatment group received GXBD (13 g crude drug/kg) for three weeks, while rats in the model control and normal control groups received equal volumes of distilled water. On the 22nd day, rats in the ischemia-reperfusion (I/R) control and GXBD-treated groups underwent 30 min occlusion of the left anterior descending (LAD) coronary artery, followed by 120 min reperfusion. Electrocardiogram was recorded, and the activities of cardiac enzymes, cytokines, and NF-kappaB were assessed after I/R.
  
  
  RESULTS
  Compared with the I/R control group, GXBD treatment restored the activity of the specific myocardial-injury marker creatine kinase (CK) and lactate dehydrogenase (LDH), and inhibited the inflammatory response involving the nuclear factor-kappaB (NF-KB) pathway, including down-regulation of interleukin (IL)-1beta and IL-6, and up-regulation of IL-10 gene expression.
  
  
  CONCLUSIONS
  GXBD strongly reduced myocardial impairment in our I/R model, including inhibition of NF-kappaB activation and inflammatory cytokine responses.
authors.affiliation: Institute of Information on Traditional Chinese Medicine, China Academy of Chinese Medical Sciences, Beijing 100700, P.R. China.
authors.forename: Hua-Min
authors.initials: HM
authors.lastname: Zhang
authors.forename: Dan-Li
authors.initials: DL
authors.lastname: Tang
authors.forename: Lin
authors.initials: L
authors.lastname: Tong
authors.forename: Ming-Jie
authors.initials: MJ
authors.lastname: Sun
authors.forename: Yu
authors.initials: Y
authors.lastname: Sui
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Zhu
authors.forename: Hong-Xin
authors.initials: HX
authors.lastname: Cao
mesh_terms: D000818:Animals; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics / Q000276:immunology; D015850:Interleukin-6 / Q000235:genetics / Q000276:immunology; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000188:drug therapy* / Q000276:immunology; D016328:NF-kappa B / Q000235:genetics / Q000276:immunology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004365:Drugs, Chinese Herbal; D015850:Interleukin-6; D016328:NF-kappa B; D016753:Interleukin-10