title: Staining membrane-bound proteins with coomassie blue r250.
abstract: INTRODUCTIONCoomassie Blue R250 permanently stains membrane-bound proteins and is compatible with PVDF and nitrocellulose membranes, but it is incompatible with nylon membranes. This technique is relatively insensitive, with a detection limit of ~1.5 μg of protein. One drawback of Coomassie Blue staining is that it produces a high background that can make interpretation of results difficult.
authors.forename: Wayne R
authors.initials: WR
authors.lastname: Stochaj
authors.forename: Tom
authors.initials: T
authors.lastname: Berkelman
authors.forename: Nancy
authors.initials: N
authors.lastname: Laird