title: A dual pH and temperature responsive polymeric fluorescent sensor and its imaging application in living cells.
abstract: A polymeric fluorescent sensor PNME, consisting of A4 and N-isopropylacrylamide (NIPAM) units, was synthesized. PNME exhibited dual responses to pH and temperature, and could be used as an intracellular pH sensor for lysosomes imaging. Moreover, it also could sense different temperature change in living cells at 25 and 37 °C, respectively.
authors.affiliation: State key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, PR China.
authors.forename: Liyan
authors.initials: L
authors.lastname: Yin
authors.forename: Chunsheng
authors.initials: C
authors.lastname: He
authors.forename: Chusen
authors.initials: C
authors.lastname: Huang
authors.forename: Weiping
authors.initials: W
authors.lastname: Zhu
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Yufang
authors.initials: Y
authors.lastname: Xu
authors.forename: Xuhong
authors.initials: X
authors.lastname: Qian
mesh_terms: D000178:Acrylamides / Q000737:chemistry*; D000180:Acrylic Resins; D002470:Cell Survival; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057054:Molecular Imaging / Q000379:methods*; D011108:Polymers / Q000737:chemistry*; D013696:Temperature
chemical_list: D000178:Acrylamides; D000180:Acrylic Resins; D005456:Fluorescent Dyes; D011108:Polymers; C052970:poly-N-isopropylacrylamide