title: Genetically encoded pH sensor for tracking surface proteins through endocytosis.
abstract: Traffic cam: a tandem dye prepared from a FRET acceptor and a fluorogenic donor functions as a cell surface ratiometric pH indicator, which upon internalization serves to follow protein trafficking during endocytosis. This sensor was used to analyze agonist-dependent internalization of β(2)-adrenergic receptors. It was also used as a surrogate antigen to reveal direct surface-to-endosome antigen transfer between dendritic cells (not shown).
authors.affiliation: Department of Biological Sciences and Molecular Biosensor and Imaging Center, Carnegie Mellon University, Pittsburgh, PA 15213, USA.
authors.forename: Anmol
authors.initials: A
authors.lastname: Grover
authors.forename: Brigitte F
authors.initials: BF
authors.lastname: Schmidt
authors.forename: Russell D
authors.initials: RD
authors.lastname: Salter
authors.forename: Simon C
authors.initials: SC
authors.lastname: Watkins
authors.forename: Alan S
authors.initials: AS
authors.lastname: Waggoner
authors.forename: Marcel P
authors.initials: MP
authors.lastname: Bruchez
mesh_terms: D000818:Animals; D017951:Antigen Presentation; D015374:Biosensing Techniques / Q000379:methods*; D003713:Dendritic Cells / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D004705:Endocytosis / Q000502:physiology*; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism*; D051379:Mice; D041681:NIH 3T3 Cells; D021381:Protein Transport
chemical_list: D005456:Fluorescent Dyes; D008565:Membrane Proteins