title: Characterization of Xenorhabdus and Photorhabdus bacteria by Fourier transform mid-infrared spectroscopy with attenuated total reflection (FT-IR/ATR).
abstract: The use of Fourier transform mid-infrared spectroscopy with attenuated total reflection for characterizing entomopathogenic bacteria from genera Xenorhabdus and Photorhabdus is evaluated for the first time. The resulting spectra of Xenorhabdus poinarii and Photorhabdus luminiscens were compared with the spectrum of Escherichia coli samples. The absorption spectra generated by the bacteria samples, were very different at the region below 1400cm(-1) which represents the stretching vibrations of phosphate and carbohydrates. Star diagrams of the fingerprint section of nematodes spectra (between 1,350 and 1,650 cm(-1)) for separation between spectra was used and showed to be a useful tool for classification purposes.
authors.affiliation: Laboratorio de Protección Vegetal, Centro de Estudios Botánicos y Agroforestales, Instituto Venezolano de Investigaciones Científicas, Calle 74, entre AV 14A y 15, Maracaibo, C.P. 4001, Bolivarian Republic of Venezuela. esanblas@yahoo.com
authors.forename: Ernesto
authors.initials: E
authors.lastname: San-Blas
authors.forename: Néstor
authors.initials: N
authors.lastname: Cubillán
authors.forename: Mayamarú
authors.initials: M
authors.lastname: Guerra
authors.forename: Edgar
authors.initials: E
authors.lastname: Portillo
authors.forename: Iván
authors.initials: I
authors.lastname: Esteves
mesh_terms: D002241:Carbohydrates / Q000032:analysis; D004926:Escherichia coli / Q000737:chemistry / Q000145:classification; D010710:Phosphates / Q000032:analysis; D020637:Photorhabdus / Q000737:chemistry* / Q000145:classification; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D020593:Xenorhabdus / Q000737:chemistry* / Q000145:classification
chemical_list: D002241:Carbohydrates; D010710:Phosphates