title: Hemoglobin immobilized with modified "fish-in-net" approach for the catalytic removal of aniline.
abstract: Blood is a waste product of the slaughter industry, while its main component hemoglobin (Hb) is a pseudo-peroxidase which is able to oxidize polycyclic aromatic hydrocarbons (PAHs) in the presence of H(2)O(2). In order to use Hb for wastewater treatment, we encapsulated it in silica-based matrix by modified "fish-in-net" approach. The as-synthesized catalysts were characterized by SEM, TEM, BET and solid-state UV-vis spectroscopy. It was found that Hb was partially homogeneously dispersed in microspheres and showed more stable peroxidase-like activity than free Hb. Moreover, it had substantially increased storage stability as well as pH stability. It was used as biocatalyst to remove aniline in aqueous solution and gave a reduction of 65% aniline removal, while 76% in the presence of additive PEG. No significant activity loss was observed after ten runs. These experimental results suggest that the resultant product was a promising biocatalyst for aromatic wastewater treatment.
authors.affiliation: Key Laboratory for Molecular Enzymology and Engineering, The Ministry of Education, Jilin University, Changchun 130012, China.
authors.forename: Jiayin
authors.initials: J
authors.lastname: Liu
authors.forename: Jingqi
authors.initials: J
authors.lastname: Guan
authors.forename: Ming
authors.initials: M
authors.lastname: Lu
authors.forename: Qiubin
authors.initials: Q
authors.lastname: Kan
authors.forename: Zhengqiang
authors.initials: Z
authors.lastname: Li
mesh_terms: D000814:Aniline Compounds / Q000302:isolation & purification*; D002384:Catalysis; D006454:Hemoglobins / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000814:Aniline Compounds; D006454:Hemoglobins; C023650:aniline