title: Activation-induced cytidine deaminase mRNA expression in oral squamous cell carcinoma-derived cell lines is upregulated by inflammatory cytokines.
abstract: Activation-induced cytidine deaminase (AID) induces cytosine deamination to generate somatic hypermutation and class switch recombnation in immunoglobulin genes. AID expression is upregulated by inflammatory cytokines such as interferon-γ and tumor necrosis factor (TNF)-α, which in turn induce p53 mutations in inflammatory or cancer cells. In this study, the effects of growth factors, cytokines or sodium butyrate on AID mRNA expression were examined in human OSCC-derived cells using real-time RT-PCR. Expression of AID mRNA was detected in OSCC cells and the expression was increased by EGF, TNF-a, or sodium butyrate. These results suggest that aberrant AID expression may play an important role in the dysplasia-carcinoma sequence in the oral cavity.
authors.affiliation: Division of Pathology, Department of Diagnostic and Therapeutic Sciences, Meikai University School of Dentistry, Sakado, Japan. y-miyazaki@dent.meikai.ac.jp
authors.forename: Yuji
authors.initials: Y
authors.lastname: Miyazaki
authors.forename: Harumi
authors.initials: H
authors.lastname: Inoue
authors.forename: Kentaro
authors.initials: K
authors.lastname: Kikuchi
authors.forename: Kuniyasu
authors.initials: K
authors.lastname: Ochiai
authors.forename: Kaoru
authors.initials: K
authors.lastname: Kusama
mesh_terms: D002087:Butyrates / Q000494:pharmacology; D002294:Carcinoma, Squamous Cell / Q000201:enzymology* / Q000235:genetics; D045744:Cell Line, Tumor; D003564:Cytidine Deaminase / Q000096:biosynthesis*; D016207:Cytokines / Q000502:physiology*; D004789:Enzyme Activation; D015972:Gene Expression Regulation, Neoplastic; D016158:Genes, p53 / Q000235:genetics; D006801:Humans; D018836:Inflammation Mediators / Q000502:physiology; D007371:Interferon-gamma / Q000502:physiology; D009154:Mutation; D012333:RNA, Messenger / Q000096:biosynthesis; D014062:Tongue Neoplasms / Q000201:enzymology* / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology; D015854:Up-Regulation; D000098830:AICDA (Activation-Induced Cytidine Deaminase)
chemical_list: D002087:Butyrates; D016207:Cytokines; D018836:Inflammation Mediators; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma; D000098830:AICDA (Activation-Induced Cytidine Deaminase); D003564:Cytidine Deaminase