title: Fibrin conduit supplemented with human mesenchymal stem cells and immunosuppressive treatment enhances regeneration after peripheral nerve injury.
abstract: To address the need for the development of bioengineered replacement of a nerve graft, a novel two component fibrin glue conduit was combined with human mesenchymal stem cells (MSC) and immunosupressive treatment with cyclosporine A. The effects of MSC on axonal regeneration in the conduit and reaction of activated macrophages were investigated using sciatic nerve injury model. A 10mm gap in the sciatic nerve of a rat was created and repaired either with fibrin glue conduit containing diluted fibrin matrix or fibrin glue conduit containing fibrin matrix with MSC at concentration of 80×10(6) cells/ml. Cells were labeled with PKH26 prior to transplantation. The animals received daily injections of cyclosporine A. After 3 weeks the distance of regeneration and area occupied by regenerating axons and ED1 positives macrophages was measured. MSC survived in the conduit and enhanced axonal regeneration only when transplantation was combined with cyclosporine A treatment. Moreover, addition of cyclosporine A to the conduits with transplanted MSC significantly reduced the ED1 macrophage reaction.
authors.affiliation: Department of Integrative Medical Biology, Section for Anatomy, Umea University, Umea, Sweden. aleksandra.mcgrath@anatomy.umu.se
authors.forename: Aleksandra M
authors.initials: AM
authors.lastname: McGrath
authors.forename: Maria
authors.initials: M
authors.lastname: Brohlin
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Kingham
authors.forename: Lev N
authors.initials: LN
authors.lastname: Novikov
authors.forename: Mikael
authors.initials: M
authors.lastname: Wiberg
authors.forename: Liudmila N
authors.initials: LN
authors.lastname: Novikova
mesh_terms: D000818:Animals; D019771:Axotomy; D001672:Biocompatible Materials / Q000627:therapeutic use; D057005:Bioengineering / Q000379:methods; D016572:Cyclosporine / Q000627:therapeutic use; D005260:Female; D015718:Fibrin Tissue Adhesive; D006801:Humans; D007166:Immunosuppressive Agents / Q000627:therapeutic use*; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D009416:Nerve Regeneration / Q000187:drug effects; D059348:Peripheral Nerve Injuries / Q000601:surgery*; D019736:Prostheses and Implants; D051381:Rats; D011916:Rats, Inbred F344; D012584:Sciatic Nerve / Q000293:injuries / Q000601:surgery
chemical_list: D001672:Biocompatible Materials; D015718:Fibrin Tissue Adhesive; D007166:Immunosuppressive Agents; D016572:Cyclosporine