title: In vitro and in vivo antimicrobial effect of photodynamic therapy using a highly pure chlorin e6 against Staphylococcus aureus Xen29.
abstract: Photodynamic therapy (PDT) has been recommended as an alternative therapy for various diseases including microbial infection. Recently, we developed a new method for the preparation of highly pure chlorin e(6) (Ce(6)), which has been widely used as a second-generation photosensitizer. PDT using Ce(6) was very effective for inhibition of in vitro growth of several bacterial strains. To clarify a possibility for its clinical application, in this study, we examined in vitro and in vivo antimicrobial effects of Ce(6)-mediated PDT in mice model of skin infection of Staphylococcus aureus Xen29. Inhibition zone analysis and colony forming unit (CFU) count revealed that Ce(6)-mediated PDT inhibited effectively in vitro bacterial growth. In addition, biofilm formation ability of S. aureus Xen29 was decreased by Ce(6)-mediated PDT. In vivo experiment, mice receiving Ce(6)-mediated PDT exhibited less intensity of bioluminescent signal, showing significant inhibition of bacterial growth. Furthermore, in histopathological examination, marked neutrophilic infiltration and massive bacterial colonies were seen in control mice and mice receiving laser or Ce(6) alone, but not in mice treated with PDT. These results suggest that PDT using Ce(6) extracted by our new method can be clinically useful against bacterial infectious diseases.
authors.affiliation: Department of Biochemistry, College of Medicine, Konyang University, Daejeon, Korea.
authors.forename: Jong-Hwan
authors.initials: JH
authors.lastname: Park
authors.forename: Mee-Young
authors.initials: MY
authors.lastname: Ahn
authors.forename: Yong-Chul
authors.initials: YC
authors.lastname: Kim
authors.forename: Soo-A
authors.initials: SA
authors.lastname: Kim
authors.forename: Yeon-Hee
authors.initials: YH
authors.lastname: Moon
authors.forename: Sang-Gun
authors.initials: SG
authors.lastname: Ahn
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Yoon
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D002735:Chlorophyllides; D015169:Colony Count, Microbial; D004195:Disease Models, Animal; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology / Q000627:therapeutic use*; D011166:Porphyrins / Q000494:pharmacology / Q000627:therapeutic use*; D012871:Skin Diseases / Q000188:drug therapy* / Q000382:microbiology / Q000473:pathology; D013203:Staphylococcal Infections / Q000188:drug therapy* / Q000382:microbiology / Q000473:pathology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development
chemical_list: D000890:Anti-Infective Agents; D002735:Chlorophyllides; D017319:Photosensitizing Agents; D011166:Porphyrins; C062985:phytochlorin