title: Efficacy of various spray disinfectants on irreversible hydrocolloid impression materials: an in vitro study.
abstract: BACKGROUND
  Most of the materials (casts, impressions, etc.) that are sent to the dental laboratories show the presence of numerous pathogenic microorganisms. All the spray disinfectants are not equally effective against these microorganisms.
  
  
  OBJECTIVE
  The aim was to compare the effectiveness of different spray disinfectants on irreversible hydrocolloid impressions and to find out the most effective dilution, contact time, and effect against each microorganism studied.
  
  
  METHODS
  The effects of four spray disinfectants, 5.25% sodium hypochlorite, 0.525% sodium hypochlorite, 1:213 (1 part in 213 parts of water) povidone iodine, and 2% glutaraldehyde along with control (distilled water) on irreversible hydrocolloid impressions contaminated with Staphylococcus aureus, Bacillus subtilis and Streptococcus viridans were studied.
  
  
  RESULTS
  Sodium hypochlorite, 5.25%, showed 1-min exposure time which was able to effect a 4 log 10 reduction in bacterial counts against S. aureus and S. viridans followed by 0.525% sodium hypochlorite and 2% glutaraldehyde for 10 min. None were able to effect a 4 log10 reduction against B. subtilis.
  
  
  CONCLUSIONS
  Sodium hypochlorite with a concentration of 5.25% was the most effective disinfectant and required the shortest contact time (1 min). Not all ADA-approved concentrations of surface disinfectants work equally well on irreversible hydrocolloid impression materials.
authors.affiliation: Department of Prosthodontics, Bapuji Dental College and Hospital, Belgaum, Karnataka, India. santoshdoddamani@rediffmail.com
authors.forename: Santosh
authors.initials: S
authors.lastname: Doddamani
authors.forename: Raghunath A
authors.initials: RA
authors.lastname: Patil
authors.forename: S A
authors.initials: SA
authors.lastname: Gangadhar
mesh_terms: D000336:Aerosols; D000464:Alginates / Q000737:chemistry*; D001412:Bacillus subtilis / Q000187:drug effects; D058491:Bacterial Load / Q000187:drug effects; D001431:Bacteriological Techniques; D003102:Colloids / Q000737:chemistry; D019551:Dental Disinfectants / Q000008:administration & dosage / Q000627:therapeutic use*; D003760:Dental Impression Materials / Q000737:chemistry*; D003761:Dental Impression Technique / Q000295:instrumentation*; D004866:Equipment Contamination / Q000517:prevention & control*; D005976:Glutaral / Q000008:administration & dosage / Q000627:therapeutic use; D006801:Humans; D008422:Materials Testing; D011206:Povidone-Iodine / Q000008:administration & dosage / Q000627:therapeutic use; D012973:Sodium Hypochlorite / Q000008:administration & dosage / Q000627:therapeutic use; D013211:Staphylococcus aureus / Q000187:drug effects; D013997:Time Factors; D034363:Viridans Streptococci / Q000187:drug effects
chemical_list: D000336:Aerosols; D000464:Alginates; D003102:Colloids; D019551:Dental Disinfectants; D003760:Dental Impression Materials; D011206:Povidone-Iodine; D012973:Sodium Hypochlorite; D005976:Glutaral