title: Typing of Lactobacillus sanfranciscensis isolates from traditional sourdoughs by combining conventional and multiplex RAPD-PCR profiles.
abstract: In the present work, a rapid and reproducible molecular method, based on the combination of conventional and multiplex RAPD-PCR reactions, was developed for typing Lactobacillus sanfranciscensis isolates from traditional sourdoughs. At first, four random primers, two used singly and two combined with the primer RD1, were chosen on the basis of their differentiating capability and reproducibility. The four resulting profiles for each isolate were integrated into a unique profile to be statistically treated by cluster analysis. The method was validated on 58 L. sanfranciscensis isolates coming from three traditional Italian sourdoughs. This new RAPD method was useful for determining the genomic diversity within the L. sanfranciscensis species. In particular, the intraspecific diversity of this species seemed to be related to the sourdough origin.
authors.affiliation: Dipartimento di Biotecnologie Agrarie, Università degli Studi di Firenze, P.le delle Cascine 24, 50144 Firenze, Italy.
authors.forename: Manuel
authors.initials: M
authors.lastname: Venturi
authors.forename: Simona
authors.initials: S
authors.lastname: Guerrini
authors.forename: Lisa
authors.initials: L
authors.lastname: Granchi
authors.forename: Massimo
authors.initials: M
authors.lastname: Vincenzini
mesh_terms: D001939:Bread / Q000382:microbiology; D017931:DNA Primers; D007778:Lactobacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D019105:Random Amplified Polymorphic DNA Technique; D015203:Reproducibility of Results
chemical_list: D017931:DNA Primers