title: Never say dye: new roles for an old fluorochrome.
abstract: Recent years have seen a remarkable increase in the number of publications dealing with the application of epifluorescence microscopy in cell biology. This can be widely attributed to the development of state-of-the-art image processing programs, as well as the development of new reagents/probes, which allow the labeling of most cell structures, organelles and metabolites with high specificity. However, the use of a specific fluorescent dye, 3,3'-dihexyloxacarbocyanine iodide (DiOC₆), has been recently revisited and several new application potentials have emerged. The goal of this mini-review is to provide an up-to-date overview of the multiple roles of this multifaceted probe.
authors.affiliation: Department of Agricultural Sciences, Biotechnology and Food Science, Cyprus University of Technology, Limassol, Cyprus. vassilis.fotopoulos@cut.ac.cy
authors.forename: Vasileios
authors.initials: V
authors.lastname: Fotopoulos
mesh_terms: D002232:Carbocyanines / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D008856:Microscopy, Fluorescence / Q000379:methods
chemical_list: D002232:Carbocyanines; D005456:Fluorescent Dyes; C007392:3,3'-dihexyl-2,2'-oxacarbocyanine