title: Live imaging of the lung.
abstract: Live imaging is critical to determining the dynamics and spatial interactions of cells within the tissue environment. In the lung, this has proven to be difficult due to the motion incurred by ventilation and cardiac contractions. In this chapter, we report protocols for imaging ex vivo live lung slices and the intact mouse lung.
authors.affiliation: Department of Pathology, University of California, San Francisco, California.
authors.forename: Emily E
authors.initials: EE
authors.lastname: Thornton
authors.affiliation: Department of Pathology, University of California, San Francisco, California.
authors.forename: Matthew F
authors.initials: MF
authors.lastname: Krummel
authors.affiliation: Department of Medicine, University of California, San Francisco, California.|Department of Laboratory Medicine, University of California, San Francisco, California.
authors.forename: Mark R
authors.initials: MR
authors.lastname: Looney
mesh_terms: D000818:Animals; D002465:Cell Movement; D003952:Diagnostic Imaging / Q000379:methods*; D005455:Fluorescent Antibody Technique; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007322:Instillation, Drug; D008168:Lung / Q000033:anatomy & histology* / Q000166:cytology / Q000601:surgery; D051379:Mice; D008853:Microscopy; D013194:Staining and Labeling
chemical_list: C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins