title: Double-stranded oligonucleotides containing 5-aminomethyl-2'-deoxyuridine form thermostable anti-parallel triplexes with single-stranded DNA or RNA.
abstract: This Letter describes the synthesis and properties of double-stranded antisense oligonucleotides connected with a pentaerythritol linker. We found that double-stranded antisense oligonucleotides with aminomethyl residues have high affinity for single-stranded DNA or RNA in buffer solutions with and without MgCl(2). Thus, these oligonucleotides would be useful as antisense oligonucleotides for targeting single-stranded RNA through triplex formation.
authors.affiliation: Department of Biomolecular Science, Faculty of Engineering, Gifu University, Yanagido, Gifu, Japan.
authors.forename: Aya
authors.initials: A
authors.lastname: Shibata
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Ueno
authors.forename: Mari
authors.initials: M
authors.lastname: Iwata
authors.forename: Haruka
authors.initials: H
authors.lastname: Wakita
authors.forename: Akira
authors.initials: A
authors.lastname: Matsuda
authors.forename: Yukio
authors.initials: Y
authors.lastname: Kitade
mesh_terms: D004247:DNA / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000737:chemistry* / Q000187:drug effects; D016503:Drug Delivery Systems; D004355:Drug Stability; D006860:Hydrogen Bonding; D006863:Hydrogen-Ion Concentration; D015636:Magnesium Chloride / Q000737:chemistry; D020320:Oligoribonucleotides, Antisense / Q000737:chemistry* / Q000494:pharmacology; D012313:RNA / Q000737:chemistry* / Q000187:drug effects; D013696:Temperature; D013936:Thymidine / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: D004277:DNA, Single-Stranded; D020320:Oligoribonucleotides, Antisense; C051802:triplex DNA; D015636:Magnesium Chloride; D012313:RNA; C023821:5-(aminomethyl)-2'-deoxyuridine; D004247:DNA; D013936:Thymidine