title: Electrically contacted enzyme based on dual hairpin DNA structure and its application for amplified detection of Hg2+.
abstract: In the present study, based on a dual hairpin DNA structure, a novel system of electrically contacted enzyme and its signal amplification for ultrasensitive detection of Hg(2+) was demonstrated. In the presence of Hg(2+), with the interaction of thymine-Hg(2+)-thymine (T-Hg(2+)-T), DNA sequence dully labeled with ferrocene (Fc) at 5' end and horseradish peroxidase (HRP) at 3' end, hybridized to the capture probe and formed the dual hairpin structure on the electrode. Fc unit acts as a relay that electrically contacts HRP with the electrode and activates the bioelectrocatalyzed reduction of H(2)O(2). And based on the bioelectrocatalyzed signal amplification of the presented system, Hg(2+) could be quantitatively detected in the range of 10(-10)-10(-6)M with a low detection limit of 52 pM. And it also demonstrated excellent selectivity against other interferential metal ions.
authors.affiliation: Key Laboratory of Chem-Biosensing, Anhui Province, PR China; Key Laboratory of Functional Molecular Solids, Anhui Province, PR China; College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China; Key Laboratory of Controllable Chemistry Reaction & Material Chemical Engineering, HeFei University of Technology, Anhui Province, Hefei 230009, PR China.
authors.forename: Guangfeng
authors.initials: G
authors.lastname: Wang
authors.affiliation: Key Laboratory of Chem-Biosensing, Anhui Province, PR China; Key Laboratory of Functional Molecular Solids, Anhui Province, PR China; College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China; Key Laboratory of Controllable Chemistry Reaction & Material Chemical Engineering, HeFei University of Technology, Anhui Province, Hefei 230009, PR China.
authors.forename: Hao
authors.initials: H
authors.lastname: Huang
authors.affiliation: Key Laboratory of Chem-Biosensing, Anhui Province, PR China; Key Laboratory of Functional Molecular Solids, Anhui Province, PR China; College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China; Key Laboratory of Controllable Chemistry Reaction & Material Chemical Engineering, HeFei University of Technology, Anhui Province, Hefei 230009, PR China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Chem-Biosensing, Anhui Province, PR China; Key Laboratory of Functional Molecular Solids, Anhui Province, PR China; College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China; Key Laboratory of Controllable Chemistry Reaction & Material Chemical Engineering, HeFei University of Technology, Anhui Province, Hefei 230009, PR China. Electronic address: wanglun@mail.ahnu.edu.cn.
authors.forename: Lun
authors.initials: L
authors.lastname: Wang
mesh_terms: D015374:Biosensing Techniques / Q000379:methods* / Q000706:statistics & numerical data; D002244:Carbon; D021881:DNA, Catalytic / Q000737:chemistry*; D055664:Electrochemical Techniques; D004800:Enzymes, Immobilized; D005296:Ferrous Compounds; D006046:Gold; D006735:Horseradish Peroxidase; D006861:Hydrogen Peroxide / Q000032:analysis; D007211:Indoles; D057230:Limit of Detection; D008628:Mercury / Q000032:analysis*; D053768:Metal Nanoparticles / Q000648:ultrastructure; D000075163:Metallocenes; D008855:Microscopy, Electron, Scanning; D054118:Nanospheres / Q000648:ultrastructure; D009690:Nucleic Acid Conformation; D010084:Oxidation-Reduction; D011108:Polymers; D013941:Thymine
chemical_list: D021881:DNA, Catalytic; D004800:Enzymes, Immobilized; D005296:Ferrous Compounds; D007211:Indoles; D000075163:Metallocenes; D011108:Polymers; C568283:polydopamine; D002244:Carbon; D006046:Gold; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; D008628:Mercury; D013941:Thymine; C004998:ferrocene