title: Antifungal activity and acute toxicity of stem bark extracts of Drypetes gossweileri S. Moore-euphorbiaceae from Cameroon.
abstract: Drypetes gossweilleri S. Moore is a plant used in traditional medicine in Cameroon. The antifungal properties of its stem-bark crude extract and fractions DG(1), DG(2), DG(3), DG(4), DG(5), DG(6), DG(7), DG(8) and DG(9) were assayed by agar and broth dilution methods on solid and liquid media against C. Krusei, C. albicans, C. glabrata, T. mentagerophytes, M. langeroinii, M. gypeum, M. audouini, T. rubrum, T. soudanense, T. terrestre, A. flavus and A. niger. The results revealed a substantial antifungal effect with minimal inhibitory concentrations ranging respectively from 24.11µg/ml to 1562µg/ml for yeasts and from 3125µg/ml to 12500µg/ml for filamentous fungi. Among the fractions, fraction DG4 exerted the highest antifungal activity. Moreover, no toxic effect was noticed in male and female albinos Wistar rats treated per os with the crude stem bark's extract of Drypetes gossweileri at a dose up to 12g/kg of body weight. The phytochemical screening of the crude extract and fractions showed the presence of alkaloids, phenols, flavonoids, saponins, anthocyanines, anthraquinones, sterols, lipids and essential oils. Therefore, Drypetes gossweileri may be safe as phytomedecine for the treatment of fungal infections.
authors.affiliation: University of Yaoundé I, Yaoundé, Cameroon. vngouana@yahoo.fr
authors.forename: Vincent
authors.initials: V
authors.lastname: Ngouana
authors.forename: Patrick Valère Tsouh
authors.initials: PV
authors.lastname: Fokou
authors.forename: Brice Ulrich Saha
authors.initials: BU
authors.lastname: Foudjo
authors.forename: Silvère Augustin
authors.initials: SA
authors.lastname: Ngouela
authors.forename: Fabrice Fekam
authors.initials: FF
authors.lastname: Boyom
authors.forename: Paul Henri Amvam
authors.initials: PH
authors.lastname: Zollo
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology / Q000633:toxicity*; D002163:Cameroon; D019665:Euphorbiaceae / Q000737:chemistry*; D005260:Female; D005658:Fungi / Q000187:drug effects*; D008297:Male; D016488:Medicine, African Traditional; D008826:Microbial Sensitivity Tests; D009181:Mycoses / Q000188:drug therapy*; D008517:Phytotherapy; D024301:Plant Bark; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D023382:Toxicity Tests, Acute
chemical_list: D000935:Antifungal Agents; D010936:Plant Extracts