title: The role of miRNAs in human papilloma virus (HPV)-associated cancers: bridging between HPV-related head and neck cancer and cervical cancer.
abstract: BACKGROUND
  Although the role of human papilloma virus (HPV) in cervical squamous cell carcinoma (CSCC) is well established, the role in head and neck SCC (HNSCC) is less clear. MicroRNAs (miRNAs) have a role in the cancer development, and HPV status may affect the miRNA expression pattern in HNSCC. To explore the influence of HPV in HNSCC, we made a comparative miRNA profile of HPV-positive (HPV+) and HPV-negative (HPV-) HNSCC against CSCC.
  
  
  METHODS
  Fresh frozen and laser microdissected-paraffin-embedded samples obtained from patients with HPV+/HPV- HNSCC, CSCC and controls were used for microarray analysis. Differentially expressed miRNAs in the HPV+ and HPV- HNSCC samples were compared with the differentially expressed miRNAs in the CSCC samples.
  
  
  RESULTS
  Human papilloma virus positive (+) HNSCC had a distinct miRNA profile compared with HPV- HNSCC. Significantly more similarity was seen between HPV+ HNSCC and CSCC than HPV- and CSCC. A set of HPV core miRNAs were identified. Of these especially the miR-15a/miR-16/miR195/miR-497 family, miR-143/miR-145 and the miR-106-363 cluster appear to be important within the known HPV pathogenesis.
  
  
  CONCLUSIONS
  This study adds new knowledge to the known pathogenic pathways of HPV and substantiates the oncogenic role of HPV in subsets of HNSCCs.
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery and Audiology, Rigshospitalet, University of Copenhagen, Denmark. christel.lajer@rh.regionh.dk
authors.forename: C B
authors.initials: CB
authors.lastname: Lajer
authors.forename: E
authors.initials: E
authors.lastname: Garnæs
authors.forename: L
authors.initials: L
authors.lastname: Friis-Hansen
authors.forename: B
authors.initials: B
authors.lastname: Norrild
authors.forename: M H
authors.initials: MH
authors.lastname: Therkildsen
authors.forename: M
authors.initials: M
authors.lastname: Glud
authors.forename: M
authors.initials: M
authors.lastname: Rossing
authors.forename: H
authors.initials: H
authors.lastname: Lajer
authors.forename: D
authors.initials: D
authors.lastname: Svane
authors.forename: L
authors.initials: L
authors.lastname: Skotte
authors.forename: L
authors.initials: L
authors.lastname: Specht
authors.forename: C
authors.initials: C
authors.lastname: Buchwald
authors.forename: F C
authors.initials: FC
authors.lastname: Nielsen
mesh_terms: D000293:Adolescent; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000821:virology; D016022:Case-Control Studies; D002648:Child; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D006258:Head and Neck Neoplasms / Q000235:genetics* / Q000821:virology; D006801:Humans; D061346:Laser Capture Microdissection; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009367:Neoplasm Staging; D020411:Oligonucleotide Array Sequence Analysis; D027383:Papillomaviridae / Q000235:genetics*; D030361:Papillomavirus Infections / Q000235:genetics* / Q000821:virology; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D004279:DNA, Viral; D035683:MicroRNAs; D012333:RNA, Messenger