title: Kineococcus glutineturens sp. nov., isolated from soil in Yunnan, south-west China.
abstract: An orange-coloured, non-spore-forming, motile and coccus-shaped actinobacterium, designated YIM 75677(T), was isolated from a soil sample collected from a dry-hot river valley in Dongchuan county, Yunnan Province, south-west China and its taxonomic position was investigated. Growth of strain YIM 75677(T) occurred at 12-55 °C, pH 6.0-9.0 and NaCl tolerance up to 2 % (w/v). Cells adhered to agar media and were agglutinated tightly together. The peptidoglycan contained meso-diaminopimelic acid, alanine and glutamic acid. The whole-cell hydrolysates mainly contained glucose, galactose, mannose and ribose. The predominant menaquinone was MK-9 (H(2)) and the major fatty acids were anteiso-C(15:0) and iso-C(15:0). Mycolic acids were not present. The DNA G+C content of strain YIM 75677(T) was 74.8 mol%. Phylogenetic analyses based on 16S rRNA gene sequence comparisons clearly revealed that strain YIM 75677(T) represents a novel member of the genus Kineococcus and is closely related to Kineococcus xinjiangensis S2-20(T) (level of similarity, 98.6 %). Meanwhile, the result of DNA-DNA hybridization between strain YIM 75677(T) and K. xinjiangensis S2-20(T) demonstrated that this isolate represented a different genomic species in the genus Kineococcus. On the basis of phylogenetic, phenotypic and chemotaxonomic characteristics, strain YIM 75677(T) represents a novel species of the genus Kineococcus, for which the name Kineococcus glutineturens sp. nov. is proposed. The type strain is YIM 75677(T) (=CCTCC AA 209075(T) = JCM 18126(T)).
authors.affiliation: College of Life Sciences, Henan Normal University, Xinxiang, People's Republic of China.
authors.forename: Guo-Xing
authors.initials: GX
authors.lastname: Nie
authors.forename: Hong
authors.initials: H
authors.lastname: Ming
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Hui-Gen
authors.initials: HG
authors.lastname: Feng
authors.forename: Shuai
authors.initials: S
authors.lastname: Li
authors.forename: Tian-Tian
authors.initials: TT
authors.lastname: Yu
authors.forename: En-Min
authors.initials: EM
authors.lastname: Zhou
authors.forename: Shu-Kun
authors.initials: SK
authors.lastname: Tang
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S