title: Cytotoxicity and biocompatibility evaluation of N,O-carboxymethyl chitosan/oxidized alginate hydrogel for drug delivery application.
abstract: In this paper, covalently cross-linked hydrogel composed of N,O-carboxymethyl chitosan and oxidized alginate was developed intending for drug delivery application. In vitro/vivo cytocompatibility and biocompatibility of the developed hydrogel were preliminary evaluated. In vitro cytocompatibility test showed that the developed hydrogel exhibited good cytocompatibility against NH3T3 cells after 3-day incubation. According to the results of acute toxicity test, there was no obvious cytotoxicity for major organs during the period of 21-day intraperitoneal administration. Meanwhile, the developed hydrogel did not induce any cutaneous reaction within 72 h of subcutaneous injection followed by slow degradation and adsorption with the time evolution. Moreover, the extraction of developed hydrogel had nearly 0% of hemolysis ratio, which indicated the good hemocompatibility of hydrogel. Based on the above results, it may be concluded that the developed N,O-carboxymethyl chitosan/oxidized alginate hydrogel with non-cytotoxicity and good biocompatibility might suitable for the various drug delivery applications.
authors.affiliation: Institute of Biomedical Engineering, School of Ophthalmology & Optometry and Eye Hospital, Wenzhou Medical College, Wenzhou, China.
authors.forename: Xingyi
authors.initials: X
authors.lastname: Li
authors.forename: Xiangye
authors.initials: X
authors.lastname: Kong
authors.forename: Zhaoliang
authors.initials: Z
authors.lastname: Zhang
authors.forename: Kaihui
authors.initials: K
authors.lastname: Nan
authors.forename: LingLi
authors.initials: L
authors.lastname: Li
authors.forename: XianHou
authors.initials: X
authors.lastname: Wang
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity; D048271:Chitosan / Q000737:chemistry*; D016503:Drug Delivery Systems; D005260:Female; D006461:Hemolysis / Q000187:drug effects; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000008:administration & dosage / Q000737:chemistry* / Q000633:toxicity*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D041681:NIH 3T3 Cells; D011817:Rabbits; D023422:Skin Irritancy Tests
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; C077199:O,N-carboxymethylchitosan; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D048271:Chitosan