title: Selenium or selenium plus folic acid-supplemented diets ameliorate renal oxidation in ethanol-exposed pups.
abstract: BACKGROUND
  Ethanol (EtOH) exposure during gestation and lactation induces an oxidative stress in offspring. In kidney, the oxidative damage is the primary pathway to alcohol-induced injury. In this study, we have demonstrated that a diet supplemented with selenium (Se) (0.5 ppm) or with Se (0.5 ppm) + folic acid (8 ppm) administered to EtOH-exposed (20% v/v) dams during gestation and lactation prevents the oxidative EtOH-provoked effects in their offspring's kidneys.
  
  
  METHODS
  All the studies were performed on 21-day-old pups. Serum, urine, and kidney Se levels were assessed by graphite-furnace atomic absorption spectrometry. Se and creatinine clearance, antioxidant enzyme activities, and lipid and protein peroxidation were determined by a spectrophotometric method in kidney.
  
  
  RESULTS
  Dietary supplementation treatments used could not improve the glomerular filtration function altered by EtOH exposure during gestation and lactation; however, they did improve renal Se deposits, renal development, and renal protein content while decreasing lipid and protein oxidation and modifying antioxidant enzymes' activity.
  
  
  CONCLUSIONS
  Se or Se + folic acid supplementations improve renal development and protein content and modify antioxidant enzymes' activity, decreasing lipid and protein oxidation after EtOH exposure. In this context, a double-supplemented diet appears to reduce protein peroxidation more efficiently than the Se-only-supplemented one, probably via superoxide dismutase and catalase.
authors.affiliation: Department of Physiology and Zoology, Faculty of Pharmacy, Seville University, Seville, Spain.
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Ojeda
authors.forename: Fatima
authors.initials: F
authors.lastname: Nogales
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Murillo
authors.forename: Olimpia
authors.initials: O
authors.lastname: Carreras
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D019587:Dietary Supplements; D004359:Drug Therapy, Combination; D000431:Ethanol / Q000633:toxicity*; D005260:Female; D005492:Folic Acid / Q000008:administration & dosage*; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D007774:Lactation / Q000187:drug effects / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012643:Selenium / Q000008:administration & dosage*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D017392:Thiobarbituric Acid Reactive Substances; D000431:Ethanol; D005492:Folic Acid; D012643:Selenium