title: Identifying brain neoplasms using dye-enhanced multimodal confocal imaging.
abstract: Brain tumors cause significant morbidity and mortality even when benign. Completeness of resection of brain tumors improves quality of life and survival; however, that is often difficult to accomplish. The goal of this study was to evaluate the feasibility of using multimodal confocal imaging for intraoperative detection of brain neoplasms. We have imaged different types of benign and malignant, primary and metastatic brain tumors. We correlated optical images with histopathology and evaluated the possibility of interpreting confocal images in a manner similar to pathology. Surgical specimens were briefly stained in 0.05 mg/ml aqueous solution of methylene blue (MB) and imaged using a multimodal confocal microscope. Reflectance and fluorescence signals of MB were excited at 642 nm. Fluorescence emission of MB was registered between 670 and 710 nm. After imaging, tissues were processed for hematoxylin and eosin (H&E) histopathology. The results of comparison demonstrate good correlation between fluorescence images and histopathology. Reflectance images provide information about morphology and vascularity of the specimens, complementary to that provided by fluorescence images. Multimodal confocal imaging has the potential to aid in the intraoperative detection of microscopic deposits of brain neoplasms. The application of this technique may improve completeness of resection and increase patient survival.
authors.affiliation: University of Massachusetts, Department of Physics and Applied Physics, One University Avenue, Lowell, Massachusetts 01854, USA.
authors.forename: Dennis
authors.initials: D
authors.lastname: Wirth
authors.forename: Matija
authors.initials: M
authors.lastname: Snuderl
authors.forename: Sameer
authors.initials: S
authors.lastname: Sheth
authors.forename: Churl-Su
authors.initials: CS
authors.lastname: Kwon
authors.forename: Matthew P
authors.initials: MP
authors.lastname: Frosch
authors.forename: William
authors.initials: W
authors.lastname: Curry
authors.forename: Anna N
authors.initials: AN
authors.lastname: Yaroslavsky
mesh_terms: D001932:Brain Neoplasms / Q000473:pathology*; D003287:Contrast Media; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005240:Feasibility Studies; D006801:Humans; D007089:Image Enhancement / Q000295:instrumentation*; D008751:Methylene Blue; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D003287:Contrast Media; D008751:Methylene Blue