title: Resting-state functional MRI: functional connectivity analysis of the visual cortex in primary open-angle glaucoma patients.
abstract: OBJECTIVE
  To analyze functional connectivity (FC) of the visual cortex using resting-state functional MRI in human primary open-angle glaucoma (POAG) patients.
  
  
  METHODS
  Twenty-two patients with known POAG and 22 age-matched controls were included in this IRB-approved study. Subjects were evaluated by 3 T MR using resting-state blood oxygenation level dependent and three-dimensional brain volume imaging (3D-BRAVO) MRI. Data processing was performed with standard software. FC maps were generated from Brodmann areas (BA) 17/18/19/7 in a voxel-wise fashion. Region of interest analysis was used to specifically examine FC among each pair of BA17/18/19/7.
  
  
  RESULTS
  Voxel-wise analyses demonstrated decreased FC in the POAG group between the primary visual cortex (BA17) and the right inferior temporal, left fusiform, left middle occipital, right superior occipital, left postcentral, right precentral gyri, and anterior lobe of the left cerebellum. Increased FC was found between BA17 and the left cerebellum, right middle cerebellar peduncle, right middle frontal gyrus, and extra-nuclear gyrus (P < 0.05). In terms of the higher visual cortices (BA18/19), positive FC was disappeared with the cerebellar vermis, right middle temporal, and right superior temporal gyri (P < 0.05). Negative FC was disappeared between BA18/19 and the right insular gyrus (P < 0.05). Region of interest analysis demonstrated no statistically significant differences in FC between the POAG patients relative to the controls (P > 0.05).
  
  
  CONCLUSIONS
  Changes in FC of the visual cortex are found in patients with POAG. These include alterations in connectivity between the visual cortex and associative visual areas along with disrupted connectivity between the primary and higher visual areas.
authors.affiliation: The First Affiliated Hospital of Soochow University, Department of Radiology, Suzhou, Jiangsu, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Dai
authors.forename: John N
authors.initials: JN
authors.lastname: Morelli
authors.forename: Fei
authors.initials: F
authors.lastname: Ai
authors.forename: Dazhi
authors.initials: D
authors.lastname: Yin
authors.forename: Chunhong
authors.initials: C
authors.lastname: Hu
authors.forename: Dongrong
authors.initials: D
authors.lastname: Xu
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Li
mesh_terms: D000328:Adult; D001284:Atrophy / Q000473:pathology; D001921:Brain / Q000473:pathology; D016022:Case-Control Studies; D002531:Cerebellum / Q000473:pathology / Q000503:physiopathology; D005260:Female; D059907:Functional Neuroimaging / Q000379:methods*; D005902:Glaucoma, Open-Angle / Q000473:pathology / Q000503:physiopathology; D006801:Humans; D021621:Imaging, Three-Dimensional; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D009929:Organ Size; D012146:Rest; D012165:Retinal Ganglion Cells / Q000473:pathology; D014793:Visual Cortex / Q000473:pathology / Q000503:physiopathology*; D014795:Visual Pathways / Q000473:pathology / Q000503:physiopathology; D014851:Wakefulness / Q000502:physiology; D055815:Young Adult