title: Fe-haem bound to Escherichia coli bacterioferritin accelerates iron core formation by an electron transfer mechanism.
abstract: BFR (bacterioferritin) is an iron storage and detoxification protein that differs from other ferritins by its ability to bind haem cofactors. Haem bound to BFR is believed to be involved in iron release and was previously thought not to play a role in iron core formation. Investigation of the effect of bound haem on formation of the iron core has been enabled in the present work by development of a method for reconstitution of BFR from Escherichia coli with exogenously added haem at elevated temperature in the presence of a relatively high concentration of sodium chloride. Kinetic analysis of iron oxidation by E. coli BFR preparations containing various amounts of haem revealed that haem bound to BFR decreases the rate of iron oxidation at the dinuclear iron ferroxidase sites but increases the rate of iron core formation. Similar kinetic analysis of BFR reconstituted with cobalt-haem revealed that this haem derivative has no influence on the rate of iron core formation. These observations argue that haem bound to E. coli BFR accelerates iron core formation by an electron-transfer-based mechanism.
authors.affiliation: Department of Biochemistry and Molecular Biology and the Centre for Blood Research, University of British Columbia, Vancouver, BC, Canada V6T 1Z3.
authors.forename: Steve G
authors.initials: SG
authors.lastname: Wong
authors.forename: Raz
authors.initials: R
authors.lastname: Abdulqadir
authors.forename: Nick E
authors.initials: NE
authors.lastname: Le Brun
authors.forename: Geoffrey R
authors.initials: GR
authors.lastname: Moore
authors.forename: A Grant
authors.initials: AG
authors.lastname: Mauk
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D003573:Cytochrome b Group / Q000378:metabolism*; D004579:Electron Transport / Q000502:physiology; D029968:Escherichia coli Proteins / Q000378:metabolism*; D005293:Ferritins / Q000378:metabolism*; D006418:Heme / Q000378:metabolism*; D007501:Iron / Q000378:metabolism*; D010084:Oxidation-Reduction; D011485:Protein Binding / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D003573:Cytochrome b Group; D029968:Escherichia coli Proteins; D006418:Heme; D005293:Ferritins; C018032:bacterioferritin; D007501:Iron