title: Overexpression of urinary N-domain ACE in chronic kidney dysfunction in Wistar rats.
abstract: Local activation of the renin-angiotensin system (RAS) has been implicated in the pathogenesis of several renal disorders. In this study we investigated how chronic kidney disease (CKD) modulates RAS components in an experimental model. Male Wistar rats were divided into three groups: sham, nephrectomized, and nephrectomized receiving losartan. Chronic kidney disease animals presented decreased renal N-domain angiotensin-converting enzyme (ACE) activity but overexpression of N-domain ACE in urine. Remnant kidneys presented high angiotensin II levels. Losartan treatment increased urine and tissue ACE activity and tissue levels of angiotensins, mainly angiotensin (1-7), and improved renal and histopathologic parameters. Taken together, the authors' results indicate that pathophysiological changes due to CKD could lead to an increased expression of somatic and N-domain ACE, mainly the 65 kDa isoform, suggesting that this enzyme could be used as a biological urinary marker in CKD.
authors.affiliation: Nephrology Division, Department of Medicine, School of Medicine, Federal University of Sao Paulo, Sao Paulo, Brazil.
authors.forename: Danielle Yuri
authors.initials: DY
authors.lastname: Arita
authors.forename: Tatiana Sousa
authors.initials: TS
authors.lastname: Cunha
authors.forename: Juliana Dinéia
authors.initials: JD
authors.lastname: Perez
authors.forename: Juliana Almada
authors.initials: JA
authors.lastname: Colucci
authors.forename: Fernanda Aparecida
authors.initials: FA
authors.lastname: Ronchi
authors.forename: Marie Doki
authors.initials: MD
authors.lastname: Nogueira
authors.forename: Lilian Saemi
authors.initials: LS
authors.lastname: Arita
authors.forename: Danielle Sanches
authors.initials: DS
authors.lastname: Aragão
authors.forename: Vicente de Paulo Castro
authors.initials: Vde P
authors.lastname: Teixeira
authors.forename: Dulce Elena
authors.initials: DE
authors.lastname: Casarini
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D019808:Losartan / Q000494:pharmacology; D008297:Male; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D051436:Renal Insufficiency, Chronic / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D012083:Renin / Q000378:metabolism*; D012084:Renin-Angiotensin System / Q000187:drug effects
chemical_list: D007703:Peptidyl-Dipeptidase A; D012083:Renin; D019808:Losartan