title: A fluorescence-based analysis of aristolochic acid-derived DNA adducts.
abstract: Aristolochic acids (AAs), major components of plant extracts from Aristolochia species, form (after metabolic activation) pro-mutagenic DNA adducts in renal tissue. The DNA adducts can be used as biomarkers for studies of AA toxicity. Identification of these adducts is a complicated and time-consuming procedure. We present here a fast, nonisotopic, fluorescence-based assay for the detection of AA-DNA adducts in multiple samples. This approach allows analysis of AA adducts in synthetic DNA with known nucleotide composition and analysis of DNA adducts formed from chemically diverse AAs in vitro. The method can be applied to compare AA-DNA adduct formation in cells and tissues.
authors.affiliation: Department of Pharmacology, State University of New York at Stony Brook, Stony Brook, NY 11777, USA. victor.romanov@sbumed.org
authors.forename: Victor
authors.initials: V
authors.lastname: Romanov
authors.forename: Victoria
authors.initials: V
authors.lastname: Sidorenko
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Rosenquist
authors.forename: Terry
authors.initials: T
authors.lastname: Whyard
authors.forename: Arthur P
authors.initials: AP
authors.lastname: Grollman
mesh_terms: D000818:Animals; D030022:Aristolochia / Q000737:chemistry*; D034341:Aristolochic Acids / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000138:chemical synthesis / Q000737:chemistry; D018736:DNA Adducts / Q000032:analysis* / Q000737:chemistry / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects; D005453:Fluorescence; D018374:LLC-PK1 Cells; D009153:Mutagens / Q000737:chemistry / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D013552:Swine
chemical_list: D034341:Aristolochic Acids; D018736:DNA Adducts; D009153:Mutagens; D010936:Plant Extracts; D004247:DNA; C000228:aristolochic acid I