title: [Humoral and cellular immune responses to HBV in the blood donors].
abstract: OBJECTIVE
  To study the magnitude and correlation of humoral and cellular immune responses to hepatitis surface antigen(HBsAg), preS1+S2 antigen, and core antigen(HBcAg) in the blood donors.
  
  
  METHODS
  Enzyme linked immunosorbent assay (ELISA) was employed to scan the humoral immune responses to HBsAg, preS1+S2 antigen, and HBcAg. Enzyme linked immunospot assay (ELISPOT) was used to check the antigen specific IFN-γ secreting cells stimulated by HBsAg, preS1+S2 antigen or HBcAg in peripheral blood mononuclear cell from blood donors.
  
  
  RESULTS
  The prevalence of humoral immune response to preS1+S2 antigen was as high as 85.7% (48/56), compared with the lower response to HBcAg (30.4%, 17/56). In the cellular immune response, there was no difference between preS1+S2 antigen and HBcAg, both of which were stronger than the response stimulated by HBsAg. PreS1+S2 antigen or HBsAg specific humoral immune and cellular immune responses were highly correlated, which could not be observed in the HBcAg specific humoral immune and cellular immune responses(P>0.05).
  
  
  CONCLUSIONS
  Either humoral or cellular immune responses to HBsAg, preS1+S2 antigen and HBcAg could be detected in the blood donors. PreS1+S2 antigen immune responses, however, were most powerful, which may suggest that PreS1+S2 antigen may be a candidate for the further HBV vaccine.
authors.affiliation: Nanjing Red Cross Blood Center, Nanjing 210003, China.
authors.forename: Yong-Lin
authors.initials: YL
authors.lastname: Yang
authors.forename: Zu-Hu
authors.initials: ZH
authors.lastname: Huang
authors.forename: Xu-Bing
authors.initials: XB
authors.lastname: Cai
authors.forename: Jie
authors.initials: J
authors.lastname: Cai
authors.forename: Gui-Ming
authors.initials: GM
authors.lastname: Ma
mesh_terms: D000328:Adult; D001782:Blood Donors; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006510:Hepatitis B Antibodies / Q000097:blood / Q000276:immunology; D006512:Hepatitis B Core Antigens / Q000276:immunology; D006514:Hepatitis B Surface Antigens / Q000276:immunology; D006515:Hepatitis B virus / Q000276:immunology*; D006801:Humans; D007111:Immunity, Cellular / Q000276:immunology*; D056724:Immunity, Humoral / Q000276:immunology*; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism; D008297:Male; D011498:Protein Precursors / Q000276:immunology; D055815:Young Adult
chemical_list: D006510:Hepatitis B Antibodies; D006512:Hepatitis B Core Antigens; D006514:Hepatitis B Surface Antigens; D011498:Protein Precursors; C050806:presurface protein 1, hepatitis B surface antigen; C050807:presurface protein 2, hepatitis B surface antigen; D007371:Interferon-gamma