title: KOMA: ELISA-microarray calibration and data analysis based on kinetic signal amplification.
abstract: Antibody microarrays with enzyme-linked immunosorbent technology are used for quantitative, simultaneous and high-throughput analysis of multiple proteins in a single probe. Kinetic detection can significantly improve precision and quantification range of microarray measurements. Here we present the open source software Kinetic Operating Microarray Analyzer (KOMA) that enables calibration and high-throughput analysis of quantitative microarray data collected using a time-resolved kinetic detection protocol of the enzymatic signal. This tool can also be helpful for analyzing data from any other analytical assays employing enzymatic signal amplification, in which a broader range of quantification is reached by the time-resolved recording of readouts. KOMA is open for download at http://www.uni-heidelberg.de/fakultaeten/biowissenschaften/ipmb/biologie/woelfl/Research.html together with a set of test raw data and requires R version 2.12 and Java RE version 6.0.
authors.affiliation: Institut für Pharmazie und Molekulare Biotechnologie, Ruperto-Carola University of Heidelberg, Heidelberg, Germany.
authors.forename: Pavlo
authors.initials: P
authors.lastname: Holenya
authors.forename: Florian
authors.initials: F
authors.lastname: Heigwer
authors.forename: Stefan
authors.initials: S
authors.lastname: Wölfl
mesh_terms: D002138:Calibration; D003627:Data Interpretation, Statistical; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards*; D019073:HT29 Cells; D006801:Humans; D040081:Protein Array Analysis / Q000592:standards*; D012984:Software