title: Anti-inflammatory and analgesic potential of a novel steroidal derivative from Bryophyllum pinnatum.
abstract: A new steroidal derivative, urs Stigmast-4, 20 (21), 23-trien-3-one and other four compounds were isolated from the leaves of Bryophyllum pinnatum. The structure of this new steroid was elucidated and established by standard spectroscopic methods. Carrageenan induced paw edema model was used for anti-inflammatory and acetic acid induced model used for analgesic activity. This new steroidal compound was found to be active in reducing inflammation (% inhibition 87.29 and 84.45 respectively) when compared with diclofenac. Further, it showed 75.72% protection in analgesic activity in acetic acid induced writhing test in mice. In conclusion the % inhibition against carrageenan induced rat paw edema and % protection against acetic acid induced writhings showed by new compound revealed that the anti-inflammatory and analgesic activity of aqueous extract B. pinnatum are mainly due to the presence of this steroidal compound.
authors.affiliation: Siddhartha Institute of Pharmacy, Dehra Dun, Uttarakhand, India.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Afzal
authors.forename: Gaurav
authors.initials: G
authors.lastname: Gupta
authors.forename: Imran
authors.initials: I
authors.lastname: Kazmi
authors.forename: Mahfoozur
authors.initials: M
authors.lastname: Rahman
authors.forename: Obaid
authors.initials: O
authors.lastname: Afzal
authors.forename: Jahangir
authors.initials: J
authors.lastname: Alam
authors.forename: Khalidur Rahman
authors.initials: KR
authors.lastname: Hakeem
authors.forename: Mohammad
authors.initials: M
authors.lastname: Pravez
authors.forename: Ritu
authors.initials: R
authors.lastname: Gupta
authors.forename: Firoz
authors.initials: F
authors.lastname: Anwar
mesh_terms: D019342:Acetic Acid; D000700:Analgesics / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D001522:Behavior, Animal / Q000187:drug effects; D002351:Carrageenan; D004008:Diclofenac / Q000627:therapeutic use; D004195:Disease Models, Animal; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004487:Edema / Q000517:prevention & control; D005260:Female; D047309:Flavones / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy*; D031962:Kalanchoe / Q000737:chemistry*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D015394:Molecular Structure; D010146:Pain / Q000139:chemically induced / Q000188:drug therapy*; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar
chemical_list: C575148:2,17-dihydroxylabda-7,13-dien-15-oic acid; D000700:Analgesics; D000893:Anti-Inflammatory Agents; D004224:Diterpenes; D047309:Flavones; D010936:Plant Extracts; D004008:Diclofenac; D002351:Carrageenan; D019342:Acetic Acid