title: Stability studies of chitosan-DNA-FAP-B nanoparticles for gene delivery to lung epithelial cells.
abstract: A successful gene delivery system requires efficiency and stability during storage. Stability studies are imperative for nanomedicines containing biotechnological products such as plasmids and targeting peptides. Chitosan-DNA-FAP-B nanoparticles are novel non-viral vectors for specific gene delivery to the lung epithelial cells. In this study, the storage stability of chitosan-DNA-FAP-B nanoparticles at -20, 5 and 24 °C was examined. Size, zeta potential and transfection efficiency of these nano-particles in storage were also evaluated. Stability studies showed that chitosan-DNA-FAP-B nanoparticles were stable after 1 month when stored at -20 °C and retained their initial size, zeta potential and transfection efficiency. However, their stability was not desirable at 5 and 24 °C. Based on these results, it can be concluded that chitosan-DNA-FAP-B nanoparticles can be a promising candidate for gene delivery to lung epithelial cells with good storage stability at -20 °C during 1 month.
authors.affiliation: Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Iran.
authors.forename: Zohreh
authors.initials: Z
authors.lastname: Mohammadi
authors.forename: Farid Abedin
authors.initials: FA
authors.lastname: Dorkoosh
authors.forename: Saman
authors.initials: S
authors.lastname: Hosseinkhani
authors.forename: Tina
authors.initials: T
authors.lastname: Amini
authors.forename: Amir Abbas
authors.initials: AA
authors.lastname: Rahimi
authors.forename: Abdolhossein Rouholamini
authors.initials: AR
authors.lastname: Najafabadi
authors.forename: Morteza Rafiee
authors.initials: MR
authors.lastname: Tehrani
mesh_terms: D018829:Adhesins, Bacterial / Q000737:chemistry / Q000378:metabolism*; D045744:Cell Line, Tumor; D048271:Chitosan / Q000737:chemistry*; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D049409:Luciferases, Firefly / Q000096:biosynthesis / Q000235:genetics; D053758:Nanoparticles; D010316:Particle Size; D020545:Respiratory Mucosa / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors; D014162:Transfection / Q000379:methods*
chemical_list: D018829:Adhesins, Bacterial; C406402:BCG fibronectin attachment protein, Mycobacterium bovis; D004247:DNA; D048271:Chitosan; D049409:Luciferases, Firefly