title: Mixture of hyaluronic acid, chondroitin 6 sulphate and dermatan sulphate used to completely regenerate bone in rat critical size defect model.
abstract: Skeletal bone losses are mainly filled with autologous graft or artificial materials. Osteoblasts are essential to maintain bone homeostasis and bone repair through a matrix synthesis. We have previously demonstrated that adherence and regenerative matrix composition are fundamental to bone healing, even in critical situations. In this work the critical size defect technique was used to evaluate the systemic activity on bone regeneration of a novel mixture of extracellular polysaccharides. A 5mm diameter hole was made in each parietal bone of male Wistar rats. The right parietal bone hole was filled with a mixture of hyaluronic acid, chondroitin 6 sulphate, and dermatan sulphate mixed with 2.5% NaCl solution, while the left hole was left free of material and untreated and considered as control. Twenty-one days after surgery, the holes and surrounding tissues were examined visually, using X-rays, and by histological staining. Using the matrix substitute, bone healing was almost complete after 21 days in the treated hole and always complete in the control side due to some systemic effect. Neovascularization was also observed along with organized trabecular bone on both sides. No abnormal bone growth or connective tissue abnormalities were noted. At the end of the experiment, 95.1% (± 3.2) bone healing (n=20) was observed on the treated side; conversely, healing bone and histological structure were better on the control side.
authors.affiliation: Service d'Anatomie Pathologique, Pôle Biologie Pathologie, CHRU University Hospital, 29609 Brest Cedex, France. philippe.zanchetta@univ-brest.fr
authors.forename: Philippe
authors.initials: P
authors.lastname: Zanchetta
authors.forename: Nicole
authors.initials: N
authors.lastname: Lagarde
authors.forename: Arnaud
authors.initials: A
authors.lastname: Uguen
authors.forename: Pascale
authors.initials: P
authors.lastname: Marcorelles
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use*; D001847:Bone Diseases / Q000188:drug therapy; D001861:Bone Regeneration / Q000187:drug effects*; D002809:Chondroitin Sulfates / Q000627:therapeutic use*; D003871:Dermatan Sulfate / Q000627:therapeutic use*; D004195:Disease Models, Animal; D006820:Hyaluronic Acid / Q000627:therapeutic use*; D008297:Male; D018919:Neovascularization, Physiologic / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects; D010294:Parietal Bone / Q000187:drug effects* / Q000473:pathology; D010521:Periosteum / Q000187:drug effects / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects
chemical_list: D001672:Biocompatible Materials; D003871:Dermatan Sulfate; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates