title: The impact of quorum sensing on the virulence of Aeromonas hydrophila and Aeromonas salmonicida towards burbot (Lota lota L.) larvae.
abstract: In this study, the link between quorum sensing in Aeromonas spp. and its virulence towards burbot (Lota lota) was investigated. High mortality occurred in burbot juveniles challenged with Aeromonas salmonicida HN-00, but not in juveniles challenged with Aeromonas hydrophila AH-1N. Meanwhile, both A. hydrophila AH-1N and A. salmonicida HN-00 were virulent towards larvae. The effect of quorum sensing on the virulence of A. hydrophila AH-1N towards burbot larvae was further investigated using quorum sensing mutants (N-(butyryl)-L-homoserine lactone production and receptor mutants). Challenge with these mutants resulted in higher survival of burbot larvae when compared to challenge with the wild type, and the addition of the signal molecule N-butyryl-L-homoserine lactone restored the virulence of the quorum sensing production mutant. Moreover, quorum sensing inhibitors protected the burbot larvae from both Aeromonas strains. Finally, the freshwater micro-algae Chlorella saccharophila and Chlamydomonas reinhardtii, which are able to interfere with quorum sensing, also protected burbot from the pathogens. However, QS interference was unlikely to be the only mechanism. This study revealed that the virulence of Aeromonas spp. towards burbot is regulated by quorum sensing and that quorum sensing inhibitors and micro-algae are promising biocontrol agents.
authors.affiliation: Laboratory of Aquaculture &Artemia Reference Center, Ghent University, Rozier 44, 9000 Gent, Belgium.
authors.forename: F M I
authors.initials: FM
authors.lastname: Natrah
authors.forename: Md Iftakharul
authors.initials: MI
authors.lastname: Alam
authors.forename: Sushant
authors.initials: S
authors.lastname: Pawar
authors.forename: A Shiri
authors.initials: AS
authors.lastname: Harzevili
authors.forename: Nancy
authors.initials: N
authors.lastname: Nevejan
authors.forename: Nico
authors.initials: N
authors.lastname: Boon
authors.forename: Patrick
authors.initials: P
authors.lastname: Sorgeloos
authors.forename: Peter
authors.initials: P
authors.lastname: Bossier
authors.forename: Tom
authors.initials: T
authors.lastname: Defoirdt
mesh_terms: D016980:Aeromonas hydrophila / Q000472:pathogenicity*; D048409:Aeromonas salmonicida / Q000472:pathogenicity*; D000818:Animals; D005393:Fish Diseases / Q000382:microbiology* / Q000401:mortality; D048388:Gadiformes; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology / Q000401:mortality / Q000662:veterinary*; D058086:Microalgae / Q000502:physiology; D053038:Quorum Sensing / Q000502:physiology*