title: Biochemical identification of rat ART-1 and Ly-1 alloantigens.
abstract: The results of this study indicate that the ART-1 and Ly-1 rat alloantigens are synonymous with each other and also with the leukocyte-common (L-C) antigen which has been previously identified as a major glycoprotein of rat thymocytes and T and B lymphocytes. This conclusion is supported by the following observations: (i) when labeling of rat lymphoid cells was studied with a fluorescence-activated cell sorter, the profiles obtained were similar for labeling with ART-1 and Ly-1 alloantibodies and a monoclonal antibody to L-C antigen: (ii) this labeling was almost completely inhibited by purified L-C antigen: (iii) preincubation with L-C antigen completely inhibited binding of the alloantibodies in a cellular radioimmunoassay; (iv) the cytotoxic effect of the alloantibodies was completely abolished by preincubation with purified L-C antigen; (v) the strain distribution of the ART-1 and Ly-1 alloantigens was identical for 11 rat strains and in linkage analysis the ART-1 and Ly-1 alloantigens were found to cosegregate. Genetic linkage studies have shown that the L-C antigen locus is unlinked to the major histocompatibility antigen (RT1), the immunoglobulin light chain (1k) and to the coat color gene (C) loci.
authors.affiliation: MRC Cellular Immunology Unit, Sir William Dunn School of Pathology, South Parks Road, Oxford OX1 3RE, U. K.
authors.forename: P B
authors.initials: PB
authors.lastname: Carter
authors.forename: C A
authors.initials: CA
authors.lastname: Sunderlandt
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000950:Antigens, Ly / Q000235:genetics* / Q000276:immunology; D002874:Chromosome Mapping; D008040:Genetic Linkage; D007147:Immunoglobulin Light Chains / Q000235:genetics; D007519:Isoantigens / Q000235:genetics* / Q000276:immunology; D017493:Leukocyte Common Antigens / Q000235:genetics* / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology; D008285:Major Histocompatibility Complex / Q000235:genetics; D010860:Pigments, Biological / Q000235:genetics; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics* / Q000276:immunology; D011863:Radioimmunoassay; D051381:Rats; D013194:Staining and Labeling
chemical_list: D000911:Antibodies, Monoclonal; D000950:Antigens, Ly; D007147:Immunoglobulin Light Chains; D007519:Isoantigens; D010860:Pigments, Biological; C499322:Parp1 protein, rat; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases; D017493:Leukocyte Common Antigens