title: Direct visualization of specifically modified extracellular glycans in living animals.
abstract: Modification patterns of heparan sulfate coordinate protein function in metazoans, yet in vivo imaging of such non-genetically encoded structures has been impossible. Here we report a transgenic method in Caenorhabditis elegans that allows direct live imaging of specific heparan sulfate modification patterns. This experimental approach reveals a dynamic and cell-specific heparan sulfate landscape and could in principle be adapted to visualize and analyze any extracellular molecule in vivo.
authors.affiliation: Department of Genetics, Albert Einstein College of Medicine, Bronx, New York, USA.
authors.forename: Matthew
authors.initials: M
authors.lastname: Attreed
authors.forename: Muriel
authors.initials: M
authors.lastname: Desbois
authors.forename: Toin H
authors.initials: TH
authors.lastname: van Kuppevelt
authors.forename: Hannes E
authors.initials: HE
authors.lastname: Bülow
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D017173:Caenorhabditis elegans / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000737:chemistry* / Q000235:genetics; D006497:Heparan Sulfate / Q000737:chemistry* / Q000235:genetics; D008856:Microscopy, Fluorescence / Q000379:methods; D057127:Single-Chain Antibodies / Q000737:chemistry* / Q000235:genetics
chemical_list: D049452:Green Fluorescent Proteins; D006497:Heparan Sulfate; D057127:Single-Chain Antibodies