title: Three-dimensional biomimetic patterning in hydrogels to guide cellular organization.
abstract: An image-guided micropatterning method is demonstrated for generating biomimetic hydrogel scaffolds with two-photon laser scanning photolithography. This process utilizes computational methods to directly translate three-dimensional cytoarchitectural features from labeled tissues into material structures. We use this method to pattern hydrogels that guide cellular organization by structurally and biochemically recapitulating complex vascular niche microenvironments with high pattern fidelity at the microscale.
authors.affiliation: Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77030, USA.
authors.forename: James C
authors.initials: JC
authors.lastname: Culver
authors.forename: Joseph C
authors.initials: JC
authors.lastname: Hoffmann
authors.forename: Ross A
authors.initials: RA
authors.lastname: Poché
authors.forename: John H
authors.initials: JH
authors.lastname: Slater
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: West
authors.forename: Mary E
authors.initials: ME
authors.lastname: Dickinson
mesh_terms: D000179:Acrylates / Q000737:chemistry; D000595:Amino Acid Sequence; D040761:Biomimetic Materials / Q000737:chemistry*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D023822:Tissue Engineering
chemical_list: D000179:Acrylates; D010455:Peptides; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011092:Polyethylene Glycols