title: Detection of p53 gene point mutation using sequence-specific molecularly imprinted PoPD electrode.
abstract: An amperometric sequence-specific molecularly imprinted single-stranded oligodeoxyribonucleotide (ss-ODN) biosensor was fabricated and characterised in this study. Using ss-ODN as the template and o-phenylenediamine as the functional monomer, the ODN biosensor was fabricated by an electropolymerisation process on an indium-tin oxide (ITO) coated glass substrate. The template ss-ODN was washed out of the ss-ODN/poly(o-phenylenediamine)(PoPD)/ITO electrode using sterilised basic ethanol-water. The resulting ss-ODN imprinted PoPD/ITO electrode was characterised using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and cyclic voltammetry (CV). The amperometric responses, i.e., Δi as a function of the target ss-ODN concentration was studied. The biosensor using ss-ODN imprinted PoPD/ITO as the working electrode showed a linear Δ current response to the target ss-ODN concentration within the range of 0.01-300 fM. The biosensor showed a sensitivity of 0.62 μA/fM, with a response time of 14s. The present novel molecularly imprinted ss-ODN biosensor could greatly benefit in terms of cost-effectiveness, storage stability, ultra sensitivity and selectivity together with the potential for improved commercial genetic sensors.
authors.affiliation: Biosensors and Bioelectronics Centre, Institute of Physics, Chemistry and Biology, IFM-Linköping University, S-58183 Linköping, Sweden; Linköping Integrative Regenerative Medicine (IGEN) Centre, IKE-Linköping University, S-581 83 Linköping, Sweden; International Center for Materials Nanoarchitectonics, National Institute for Materials Science, 1-2-1, Sengen, Tsukuba, Ibaraki 305 0047, Japan. Electronic address: ashutosh.tiwari@liu.se.
authors.forename: Ashutosh
authors.initials: A
authors.lastname: Tiwari
authors.affiliation: Biosensors and Bioelectronics Centre, Institute of Physics, Chemistry and Biology, IFM-Linköping University, S-58183 Linköping, Sweden.
authors.forename: Swapneel R
authors.initials: SR
authors.lastname: Deshpande
authors.affiliation: International Center for Materials Nanoarchitectonics, National Institute for Materials Science, 1-2-1, Sengen, Tsukuba, Ibaraki 305 0047, Japan; JSR-CREST, 5, Sanbancho, Chiyoda-ku, Tokyo 102-0075, Japan.
authors.forename: Hisatoshi
authors.initials: H
authors.lastname: Kobayashi
authors.affiliation: Biosensors and Bioelectronics Centre, Institute of Physics, Chemistry and Biology, IFM-Linköping University, S-58183 Linköping, Sweden. Electronic address: anthony.turner@liu.se.
authors.forename: Anthony P F
authors.initials: APF
authors.lastname: Turner
mesh_terms: D015374:Biosensing Techniques / Q000379:methods* / Q000706:statistics & numerical data; D004252:DNA Mutational Analysis / Q000379:methods; D055664:Electrochemical Techniques; D004566:Electrodes; D016158:Genes, p53; D006801:Humans; D057230:Limit of Detection; D008855:Microscopy, Electron, Scanning; D054802:Molecular Imprinting / Q000379:methods*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D010655:Phenylenediamines / Q000737:chemistry; D017354:Point Mutation; D017550:Spectroscopy, Fourier Transform Infrared; D017971:Tin Compounds
chemical_list: D009838:Oligodeoxyribonucleotides; D010655:Phenylenediamines; D017971:Tin Compounds; C530487:poly(o-phenylenediamine); C109984:indium tin oxide