title: Intracerebroventricular transplantation of human bone marrow-derived multipotent progenitor cells in an immunodeficient mouse model of mucopolysaccharidosis type I (MPS-I).
abstract: Mucopolysaccharidosis type I (MPS-I; Hurler syndrome) is an inborn error of metabolism caused by lack of the functional lysosomal glycosaminoglycan (GAG)-degrading enzyme α-L-iduronidase (IDUA). Without treatment, the resulting GAG accumulation causes multisystem dysfunction and death within the first decade. Current treatments include allogeneic hematopoietic stem cell transplantation (HSCT) and enzyme replacement therapy. HSCT ameliorates clinical features and extends life but is not available to all patients, and inadequately corrects the most devastating features of the disease including mental retardation and skeletal deformities. Recent developments suggest that stem cells can be used to deliver needed enzymes to the central nervous system. To test this concept, we transplanted bone marrow-derived normal adult human MultiStem® cells into the cerebral lateral ventricles of immunodeficient MPS-I neonatal mice. Transplanted cells and human-specific DNA were detected in the hippocampal formation, striatum, and other areas of the central nervous system. Brain tissue assays revealed significant long-term decrease in GAG levels in the hippocampus and striatum. Sensorimotor testing 6 months after transplantation demonstrated significantly improved rotarod performance of transplanted mice in comparison to nontransplanted and sham-transplanted control animals. These results suggest that a single injection of MultiStem cells into the cerebral ventricles of neonatal MPS-I mice induces sustained reduction in GAG accumulation within the brain, and modest long-term improvement in sensorimotor function.
authors.affiliation: Department of Neurosurgery, University of Minnesota, Minneapolis, MN 55455, USA.
authors.forename: Zhenhong
authors.initials: Z
authors.lastname: Nan
authors.forename: Laurie
authors.initials: L
authors.lastname: Shekels
authors.forename: Oleg
authors.initials: O
authors.lastname: Ryabinin
authors.forename: Carrie
authors.initials: C
authors.lastname: Evavold
authors.forename: Matthew S
authors.initials: MS
authors.lastname: Nelson
authors.forename: Shaukat A
authors.initials: SA
authors.lastname: Khan
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Deans
authors.forename: Robert W
authors.initials: RW
authors.lastname: Mays
authors.forename: Walter C
authors.initials: WC
authors.lastname: Low
authors.forename: Pankaj
authors.initials: P
authors.lastname: Gupta
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001854:Bone Marrow Cells / Q000166:cytology*; D001921:Brain / Q000378:metabolism; D003342:Corpus Striatum / Q000378:metabolism; D004195:Disease Models, Animal; D056228:Feedback, Sensory / Q000502:physiology; D006025:Glycosaminoglycans / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism; D006801:Humans; D057967:Infusions, Intraventricular; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D009043:Motor Activity / Q000502:physiology; D008059:Mucopolysaccharidosis I / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D039902:Multipotent Stem Cells / Q000166:cytology / Q000637:transplantation*; D014183:Transplantation, Heterologous
chemical_list: D006025:Glycosaminoglycans