title: Digestion of Insoluble Protein Fractions for Multidimensional Protein Identification Technology (MuDPIT) Analysis.
abstract: INTRODUCTIONProteins associated with insoluble particulate matter from a whole-cell lysate, or carefully prepared membrane samples, can be analyzed using MuDPIT analysis. However, the preliminary steps provided in this protocol are required to prepare a usable complex protein mixture from these types of samples.
authors.forename: David M
authors.initials: DM
authors.lastname: Schieltz
authors.forename: Michael P
authors.initials: MP
authors.lastname: Washburn