title: Size exclusion chromatography for the removal of pigments from extracellular ligninolytic enzyme extracts from decayed wheat straw.
abstract: Solid-state fermentation of wheat straw was carried out by a native white rot basidiomycete Daedaleopsis flavida strain 5A. Extract prepared from the 12-day decayed wheat straw contained extracellular ligninolytic enzymes like manganese peroxidase (MnP), manganese-independent peroxidase (MIP), lignin peroxidase (LiP) and laccase along with straw-degraded products and pigments. Sephacryl S-200 size exclusion chromatography in 16/100 column was used for the separation of these ligninolytic enzymes and straw-degraded products and pigments. Recovery of pigment-free ligninolytic enzyme activities as protein was 40% of the total proteins loaded and specific LiP activity increased 34 fold after size exclusion chromatography. Thus accurate estimation of LiP by veratryl alcohol oxidation assay was possible only after the removal of interfering pigments. The reproducibility of size exclusion chromatography is adjudged satisfactory from the consistent results obtained after seven repetitive uses of matrices.
authors.affiliation: Department of Biosciences, Saurashtra University, Rajkot, 360 005, India.
authors.forename: Dharmendra
authors.initials: D
authors.lastname: Shukla
authors.forename: Bhavesh
authors.initials: B
authors.lastname: Patel
authors.forename: Hasmukh
authors.initials: H
authors.lastname: Modi
authors.forename: Bharat Rajiv Manuel
authors.initials: BR
authors.lastname: Vyas
mesh_terms: D001487:Basidiomycota / Q000378:metabolism; D005591:Chemical Fractionation; D002850:Chromatography, Gel / Q000379:methods*; D005285:Fermentation; D008031:Lignin / Q000378:metabolism; D010860:Pigments, Biological / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D018547:Plant Stems / Q000737:chemistry*; D014908:Triticum / Q000737:chemistry*
chemical_list: D010860:Pigments, Biological; D010936:Plant Extracts; D008031:Lignin