title: Intrathymic programming of effector fates in three molecularly distinct γδ T cell subtypes.
abstract: Innate γδ T cells function in the early phase of immune responses. Although innate γδ T cells have often been studied as one homogenous population, they can be functionally classified into effector subsets on the basis of the production of signature cytokines, analogous to adaptive helper T cell subsets. However, unlike the function of adaptive T cells, γδ effector T cell function correlates with genomically encoded T cell antigen receptor (TCR) chains, which suggests that clonal TCR selection is not the main determinant of the differentiation of γδ effector cells. A high-resolution transcriptome analysis of all emergent γδ thymocyte subsets segregated on the basis of use of the TCR γ-chain or δ-chain indicated the existence of three separate subtypes of γδ effector cells in the thymus. The immature γδ subsets were distinguished by unique transcription-factor modules that program effector function.
authors.affiliation: Department of Pathology, University of Massachusetts Medical School, Worcester, Massachusetts, USA.
authors.forename: Kavitha
authors.initials: K
authors.lastname: Narayan
authors.forename: Katelyn E
authors.initials: KE
authors.lastname: Sylvia
authors.forename: Nidhi
authors.initials: N
authors.lastname: Malhotra
authors.forename: Catherine C
authors.initials: CC
authors.lastname: Yin
authors.forename: Gregory
authors.initials: G
authors.lastname: Martens
authors.forename: Therese
authors.initials: T
authors.lastname: Vallerskog
authors.forename: Hardy
authors.initials: H
authors.lastname: Kornfeld
authors.forename: Na
authors.initials: N
authors.lastname: Xiong
authors.forename: Nadia R
authors.initials: NR
authors.lastname: Cohen
authors.forename: Michael B
authors.initials: MB
authors.lastname: Brenner
authors.forename: Leslie J
authors.initials: LJ
authors.lastname: Berg
authors.forename: Joonsoo
authors.initials: J
authors.lastname: Kang
mesh_terms: D000367:Age Factors; D000818:Animals; D051927:CD24 Antigen / Q000276:immunology / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology*; D019070:Cell Lineage / Q000276:immunology; D005333:Fetus / Q000166:cytology / Q000276:immunology; D005434:Flow Cytometry; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism; D020381:Interleukin-17 / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018448:Models, Immunological; D009169:Mycobacterium tuberculosis / Q000276:immunology / Q000378:metabolism; D025341:Principal Component Analysis; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000145:classification / Q000235:genetics* / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D014157:Transcription Factors / Q000276:immunology / Q000378:metabolism; D059467:Transcriptome / Q000235:genetics / Q000276:immunology*
chemical_list: D051927:CD24 Antigen; C497890:Cd24a protein, mouse; D020381:Interleukin-17; D016692:Receptors, Antigen, T-Cell, gamma-delta; D014157:Transcription Factors; D007371:Interferon-gamma