title: Food restriction and refeeding induces changes in lipid pathways and fat deposition in the adipose and hepatic tissues in rats with diet-induced obesity.
abstract: The aim of this study was to determine the effects of successive cycles of a moderately restrictive diet and refeeding with a high-fat diet on the metabolism of the adipose and hepatic tissues of obese rats. Rats were assigned to the following groups: a chow diet; a high-fat diet; a moderate caloric restriction; or a moderate caloric restriction plus refeeding. Some animals in each group were given [1-(14)C]triolein intragastrically, while others received an intraperitoneal injection of 3 mCi (3)H(2)O. All animals were killed by decapitation. The retroperitoneal, visceral epididymal and omental white adipose tissues, brown adipose tissue, liver and blood were immediately removed. The lipid uptake from the diet, in vivo rate of lipogenesis, percentage of fat, lipid profile and leptin concentration were analysed. The high-fat diet promoted an increase in fatty liver (P ≤ 0.05), adiposity mass (P ≤ 0.05) and the plasma concentration of leptin (P ≤ 0.05) and a decreased lipid uptake in white adipose tissue depots (P ≤ 0.05) in relation to the chow diet. The moderate caloric restriction did not reverse the changes promoted by the high-fat diet but induced a small decrease in adiposity, which was reversed after refeeding, and the animals maintained a dyslipidaemic profile and high fat deposition in the liver. We can conclude that the high-fat diet and subsequent moderate caloric restriction plus refeeding increased the risks of developing visceral obesity, dyslipidaemia and non-alcoholic fatty liver disease, which suggests that this type of experimental protocol can be used to study mechanisms related to the metabolic syndrome.
authors.affiliation: Departamento de Educação Física e Motricidade-DEFMH, Universidade Federal de São Carlos-UFSCar Rodovia Washington Luís, Km 235-SP-310, São Carlos-São Paulo-Brasil, CEP: 13565-905. duarte_reame@yahoo.com.br
authors.forename: Fernanda Oliveira
authors.initials: FO
authors.lastname: Duarte
authors.forename: Marcela
authors.initials: M
authors.lastname: Sene-Fiorese
authors.forename: Nadia Carla
authors.initials: NC
authors.lastname: Cheik
authors.forename: Adriana Simone Lopes Santa
authors.initials: AS
authors.lastname: Maria
authors.forename: Antônio Eduardo
authors.initials: AE
authors.lastname: de Aquino
authors.forename: Jorge Camargo
authors.initials: JC
authors.lastname: Oishi
authors.forename: Elizeu Antônio
authors.initials: EA
authors.lastname: Rossi
authors.forename: Ana Cláudia
authors.initials: AC
authors.lastname: Garcia de Oliveira Duarte
authors.forename: Ana Raimunda
authors.initials: AR
authors.lastname: Dâmaso
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D050154:Adiposity; D000818:Animals; D031204:Caloric Restriction; D059305:Diet, High-Fat; D005502:Food; D005508:Food Deprivation / Q000502:physiology*; D008099:Liver / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar