title: [Correlation between serum anti-P53 and familial clustering of hepatocellular carcinoma in Guangxi].
abstract: OBJECTIVE
  To assess the correlation between familial clustering of hepatocellular carcinoma (HCC) and the level of anti-P53 in human serum in Guangxi.
  
  
  METHODS
  Enzyme-linked immunosorbent assay (ELISA) was used to detect anti-P53 in 164 members from 20 HCC families and 164 members from non-cancer control families. Univariate analysis was performed to assess the correlation between seral level of P53 antibody and familial clustering of HCC.
  
  
  RESULTS
  The level of P53 antibody was significantly higher in the members of HCC families than controls (Z=-3.04, P=0.002). After eliminating the interference of hepatitis B virus infection, this tendency still remains (P=0.011).  And there was a significant difference between relatives of different degrees from HCC families (chi-square=11.593, P=0.021), with the expression of anti-P53 declining along with decrease in relationship coefficient. Furthermore, the number of individuals with high anti-P53 expression was also significantly greater in HCC families (95/164) than controls (71/164) (P=0.006). And the expression was rising along with the increasing HCC numbers (chi-square=16.068, P=0.000).  Anti-P53 level was also greater in HCC families featuring sibling affection than parental affection (chi-square=12.679, P=0.002). Univariate analysis indicated that high expression of anti-P53 is a risk factor for development of HCC (OR=2.087, 95%CI: 1.270-3.431).
  
  
  CONCLUSIONS
  High level of anti-P53 expression may be a factor for the clustering of HCC families in Guangxi, China.
authors.affiliation: Department of Infectious Diseases, Guangxi Medical University, Nanning, Guangxi, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Pang
authors.forename: Guo-jian
authors.initials: GJ
authors.lastname: Li
authors.forename: Ji-zhou
authors.initials: JZ
authors.lastname: Wu
authors.forename: Jian-lin
authors.initials: JL
authors.lastname: Wu
authors.forename: Wu-qing
authors.initials: WQ
authors.lastname: Chen
authors.forename: Qiu-yue
authors.initials: QY
authors.lastname: Ning
authors.forename: Ying-hua
authors.initials: YH
authors.lastname: Wei
authors.forename: Die-fei
authors.initials: DF
authors.lastname: Hu
authors.forename: Ling
authors.initials: L
authors.lastname: Qin
mesh_terms: D000293:Adolescent; D000328:Adult; D000912:Antibodies, Neoplasm / Q000097:blood* / Q000235:genetics; D006528:Carcinoma, Hepatocellular / Q000097:blood / Q000235:genetics* / Q000276:immunology*; D002648:Child; D002681:China; D016000:Cluster Analysis; D005192:Family Health; D005260:Female; D006801:Humans; D008113:Liver Neoplasms / Q000097:blood / Q000235:genetics* / Q000276:immunology*; D008297:Male; D012307:Risk Factors; D016159:Tumor Suppressor Protein p53 / Q000276:immunology*; D055815:Young Adult
chemical_list: D000912:Antibodies, Neoplasm; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53