title: Plasma membrane calcium ATPase expression in human colon multistep carcinogenesis.
abstract: The expression of the plasma membrane Ca(2+) ATPase (PMCA) was analyzed in a series of 84 formalin-fixed and paraffin embedded colon samples including normal mucosa (n = 32), adenoma (n = 19), adenocarcinoma (n = 27), and lymph node metastasis (n = 6) using (i) immunohistochemistry, (ii) mRNA in situ hybridization, and (iii) quantitative reverse-transcriptase PCR. A marked reduction of PMCA4 protein was observed in high-grade adenoma, colon cancer as well as lymph node metastasis, pointing to its potential role in the progression of cancer. However, PMCA4 RNA transcripts were unchanged or even increased in colon carcinomas, suggesting posttranscriptional regulation of PMCA4 during carcinogenesis.
authors.affiliation: Department of Anatomy and Cell Biology, Philipps-University, Marburg, Germany.
authors.forename: Jan H
authors.initials: JH
authors.lastname: Rüschoff
authors.forename: Timo
authors.initials: T
authors.lastname: Brandenburger
authors.forename: Emanuel E
authors.initials: EE
authors.lastname: Strehler
authors.forename: Adelaida G
authors.initials: AG
authors.lastname: Filoteo
authors.forename: Ernst
authors.initials: E
authors.lastname: Heinmöller
authors.forename: Gerhard
authors.initials: G
authors.lastname: Aumüller
authors.forename: Beate
authors.initials: B
authors.lastname: Wilhelm
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology* / Q000473:pathology; D000236:Adenoma / Q000201:enzymology* / Q000473:pathology; D000368:Aged; D002462:Cell Membrane / Q000201:enzymology; D003110:Colonic Neoplasms / Q000201:enzymology* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000201:enzymology* / Q000473:pathology; D008207:Lymphatic Metastasis / Q000473:pathology; D008297:Male; D053499:Plasma Membrane Calcium-Transporting ATPases / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction / Q000502:physiology; D046888:Tissue Array Analysis
chemical_list: D012333:RNA, Messenger; D053499:Plasma Membrane Calcium-Transporting ATPases