title: Evaluation of polyphenolic fraction isolated from aerial parts of Tribulus pterocarpus on biological properties of blood platelets in vitro.
abstract: The antiplatelet and antioxidative activity of polyphenolic fraction isolated from aerial parts of Tribulus pterocarpus in blood platelets stimulated by thrombin was studied. Thrombin as a strong physiological agonist induces the enzymatic peroxidation of endogenous arachidonic acid, the formation of different reactive oxygen species, including superoxide anion radicals ([Formula: see text](·)) and the platelet aggregation. Therefore, the aim of our study was to assess if the polyphenolic fraction from aerial parts of T. pterocarpus may change the biological properties of blood platelets activated by thrombin. We used cytochrome c reduction method to test the ability of this fraction to change [Formula: see text](·) generation in platelets. Arachidonic acid metabolism was measured by the level of thiobarbituric acid reactive substances (TBARS) and by the production of 8-epi-prostaglandin (8-EPI) F(2). Moreover, we determined the effects of the fraction on blood platelet aggregation induced by thrombin. We observed that the polyphenolic fraction from T. pterocarpus reduced [Formula: see text](·), 8-EPI and TBARS production in these cells. The ability of the fraction to decrease the [Formula: see text](·) generation in blood platelets supports the importance of free radicals in platelet functions, including aggregation process. This study may suggest that the tested plant fraction might be a good candidate for protecting blood platelets against changes of their biological functions, which may be associated with the pathogenesis of different cardiovascular disorders.
authors.affiliation: Department of General Biochemistry, University of Lodz , Pomorska 141/3, 90-236 Lodz, Poland. olasb@biol.uni.lodz.pl
authors.forename: Beata
authors.initials: B
authors.lastname: Olas
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Morel
authors.forename: Arafa I
authors.initials: AI
authors.lastname: Hamed
authors.forename: Wieslaw
authors.initials: W
authors.lastname: Oleszek
authors.forename: Anna
authors.initials: A
authors.lastname: Stochmal
mesh_terms: D001792:Blood Platelets / Q000187:drug effects* / Q000502:physiology*; D006801:Humans; D035261:Plant Components, Aerial / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D015539:Platelet Activation / Q000187:drug effects; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D059808:Polyphenols / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013917:Thrombin / Q000494:pharmacology; D032444:Tribulus / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D010975:Platelet Aggregation Inhibitors; D059808:Polyphenols; D017382:Reactive Oxygen Species; D013917:Thrombin