title: An approach for differentiating isozymes. Construction of libraries containing short aromatic peptides as part of a method to design selective inhibitors against lipases.
abstract: Through synthesis and assays of peptidyl substrates, we could select substrates having peptidyl complementary against lipases. The best substrate showed 20-fold improved K(m) relative to non-peptidyl substrate. Using this information, we generated selective inhibitors. Lipase activities with peptidyl substrates were represented as fingerprints. Differences in fingerprints reflect different structures near active site of lipases, could be used for generating selective inhibitors.
authors.affiliation: Department of Chemistry & Education, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Sangeun
authors.initials: S
authors.lastname: Park
authors.forename: Jaehoon
authors.initials: J
authors.lastname: Yu
mesh_terms: D024322:Amino Acids, Aromatic; D004791:Enzyme Inhibitors; D007527:Isoenzymes; D007700:Kinetics; D008049:Lipase / Q000037:antagonists & inhibitors*; D008722:Methods; D010455:Peptides; D054852:Small Molecule Libraries / Q000138:chemical synthesis*; D013379:Substrate Specificity
chemical_list: D024322:Amino Acids, Aromatic; D004791:Enzyme Inhibitors; D007527:Isoenzymes; D010455:Peptides; D054852:Small Molecule Libraries; D008049:Lipase