title: Development of 198 novel EST-derived microsatellites in Eucalyptus (Myrtaceae).
abstract: OBJECTIVE
  Expressed sequence tag (EST)-derived microsatellites were identified in Eucalyptus through screening the GenBank database. The loci were sequence-verified and explored for polymorphism among 20 genotypes.
  
  
  RESULTS
  In total, 198 novel microsatellites were developed from 8262 unigenes, with the identity of 73.6-100% to the original sequences and presence of the expected repeat motifs. One hundred and eighty-four markers proved to be polymorphic among 10 E. urophylla and 10 E. tereticornis genotypes, with the number of alleles per locus, observed heterozygosity, and polymorphic information content being 2-17 (mean: 7.11), 0-1.0 (mean: 0.4511), and 0.0940-0.9131 (mean: 0.6571), respectively.
  
  
  CONCLUSIONS
  These markers will be useful for germplasm characterization, genome mapping, and gene tagging for economic traits in the two species examined and may have potential for genetic applications in Eucalyptus.
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Yiheyuan Hou, Beijing, People's Republic of China.
authors.forename: Xudong
authors.initials: X
authors.lastname: He
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Fagen
authors.initials: F
authors.lastname: Li
authors.forename: Qijie
authors.initials: Q
authors.lastname: Weng
authors.forename: Mei
authors.initials: M
authors.lastname: Li
authors.forename: Li-an
authors.initials: LA
authors.lastname: Xu
authors.forename: Jisen
authors.initials: J
authors.lastname: Shi
authors.forename: Siming
authors.initials: S
authors.lastname: Gan
mesh_terms: D000483:Alleles; D005052:Eucalyptus / Q000235:genetics*; D020224:Expressed Sequence Tags; D005838:Genotype; D018895:Microsatellite Repeats / Q000235:genetics*; D011110:Polymorphism, Genetic