title: A combined approach of high-throughput sequencing and degradome analysis reveals tissue specific expression of microRNAs and their targets in cucumber.
abstract: MicroRNAs (miRNAs) are endogenous small RNAs playing an important regulatory function in plant development and stress responses. Among them, some are evolutionally conserved in plant and others are only expressed in certain species, tissue or developmental stages. Cucumber is among the most important greenhouse species in the world, but only a limited number of miRNAs from cucumber have been identified and the experimental validation of the related miRNA targets is still lacking. In this study, two independent small RNA libraries from cucumber leaves and roots were constructed, respectively, and sequenced with the high-throughput Illumina Solexa system. Based on sequence similarity and hairpin structure prediction, a total of 29 known miRNA families and 2 novel miRNA families containing a total of 64 miRNA were identified. QRT-PCR analysis revealed that some of the cucumber miRNAs were preferentially expressed in certain tissues. With the recently developed 'high throughput degradome sequencing' approach, 21 target mRNAs of known miRNAs were identified for the first time in cucumber. These targets were associated with development, reactive oxygen species scavenging, signaling transduction and transcriptional regulation. Our study provides an overview of miRNA expression profile and interaction between miRNA and target, which will help further understanding of the important roles of miRNAs in cucumber plants.
authors.affiliation: Department of Horticulture, Zhejiang University, Hangzhou, China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Mao
authors.forename: Zeyun
authors.initials: Z
authors.lastname: Li
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Xia
authors.forename: Yadan
authors.initials: Y
authors.lastname: Li
authors.forename: Jingquan
authors.initials: J
authors.lastname: Yu
mesh_terms: D001483:Base Sequence; D018553:Cucumis sativus / Q000235:genetics*; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D059014:High-Throughput Nucleotide Sequencing / Q000295:instrumentation / Q000379:methods*; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D018515:Plant Leaves / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics; D018517:Plant Roots / Q000235:genetics; D018749:RNA, Plant / Q000737:chemistry / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018076:DNA, Complementary; D035683:MicroRNAs; D010940:Plant Proteins; D018749:RNA, Plant