title: Whispering gallery mode aptasensors for detection of blood proteins.
abstract: Whispering gallery mode resonators (WGMR), as silica microspheres, have been recently proposed as an efficient tool for the realisation of optical biosensors. In this work we present a functionalization procedure based on the DNA-aptamer sequence immobilization on WGMR, able to recognize specifically thrombin or VEGF protein, preserving a high Q factor. The protein binding was optically characterized in terms of specificity in buffer solution or in 10% diluted human serum. Simulation of the protein flow was found in good agreement with experimental data. The aptasensor was also chemically regenerated and tested again, demonstrating the reusability of our system.
authors.affiliation: FBK-CMM, Via Sommarive 18, 38123, Trento, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Pasquardini
authors.forename: Simone
authors.initials: S
authors.lastname: Berneschi
authors.forename: Andrea
authors.initials: A
authors.lastname: Barucci
authors.forename: Franco
authors.initials: F
authors.lastname: Cosi
authors.forename: Ramona
authors.initials: R
authors.lastname: Dallapiccola
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Insinna
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Lunelli
authors.forename: Gualtiero Nunzi
authors.initials: GN
authors.lastname: Conti
authors.forename: Cecilia
authors.initials: C
authors.lastname: Pederzolli
authors.forename: Simone
authors.initials: S
authors.lastname: Salvadori
authors.forename: Silvia
authors.initials: S
authors.lastname: Soria
mesh_terms: D052157:Aptamers, Nucleotide; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D001798:Blood Proteins / Q000032:analysis*; D004867:Equipment Design; D006801:Humans; D061925:Immobilized Nucleic Acids; D046210:Microfluidic Analytical Techniques; D018625:Microscopy, Atomic Force; D008863:Microspheres; D055620:Optical Phenomena; D012822:Silicon Dioxide; D013917:Thrombin / Q000032:analysis; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis / Q000097:blood
chemical_list: D052157:Aptamers, Nucleotide; D001798:Blood Proteins; D061925:Immobilized Nucleic Acids; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D012822:Silicon Dioxide; D013917:Thrombin