title: Cisplatin disrupts the latency of human herpesvirus 8 and induces apoptosis in primary effusion lymphoma cells.
abstract: Human herpesvirus 8 (HHV8) is the etiologic agent for primary effusion lymphoma (PEL). The aim of this study is to investigate the effects of cisplatin on the PEL cells. Cisplatin treatment induced apoptosis and inhibited the growth of PEL cells, and the effect was more profound in the HHV8-positive lymphoma cells compared with the EBV-positive lymphoma cells. Cisplatin treatment decreased the expression of HHV8 latent genes and activated p53 at serine 15 in PEL cells. Our results indicate that cisplatin can disrupt HHV8 latency and induce reactivation of p53 and highly selective treatment modality for this virally induced lymphoma.
authors.affiliation: Department of Medical Laboratory Sciences and Biotechnology, Fooyin-University, Kaohsiung, Taiwan, ROC. yfwang@mail.fy.edu.tw
authors.forename: Yi-Fen
authors.initials: YF
authors.lastname: Wang
authors.forename: Yee-Hsuan
authors.initials: YH
authors.lastname: Chiou
authors.forename: Lin-Yu
authors.initials: LY
authors.lastname: Wang
authors.forename: Su-Ting
authors.initials: ST
authors.lastname: Chang
authors.forename: Huey-Wen
authors.initials: HW
authors.lastname: Shyu
authors.forename: Chang-Yu
authors.initials: CY
authors.lastname: Chen
authors.forename: Kuan-Hua
authors.initials: KH
authors.lastname: Lin
authors.forename: Miao-Chen
authors.initials: MC
authors.lastname: Chou
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002469:Cell Separation; D002945:Cisplatin / Q000494:pharmacology*; D005434:Flow Cytometry; D015870:Gene Expression / Q000187:drug effects; D005814:Genes, Viral / Q000187:drug effects; D019288:Herpesvirus 8, Human / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D054685:Lymphoma, Primary Effusion / Q000378:metabolism / Q000821:virology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D017735:Virus Latency / Q000187:drug effects*
chemical_list: D000970:Antineoplastic Agents; D016159:Tumor Suppressor Protein p53; D002945:Cisplatin