title: Gold-silver-nanoclusters having cholic acid imprinted nanoshell.
abstract: Molecular imprinted polymers (MIPs) as a recognition element for sensors are increasingly of interest and MIP-nanoparticles have started to appear in the literature. In this study, we have proposed a novel thiol ligand-capping method with polymerizable methacryloylamido-cysteine (MAC) attached to gold-silver-nanoclusters reminiscent of a self-assembled monolayer and have reconstructed surface shell by synthetic host polymers based on molecular imprinting method for cholic acid recognition. In this method, methacryloylamidohistidine-Pt(II) [MAH-Pt(II)] has used as a new metal-chelating monomer via metal coordination-chelation interactions and cholic acid. Nanoshell sensors with templates give a cavity that is selective for cholic acid. The cholic acid can simultaneously chelate to Pt(II) metal ion and fit into the shape-selective cavity. Thus, the interaction between Pt(II) ion and free coordination spheres has an effect on the binding ability of the gold-silver-nanoclusters nanosensor. The binding affinity of the cholic acid imprinted nanoparticles have investigated by using the Langmuir and Scatchard methods and determined affinity constant (K(affinity)) has found to be 2.73 × 10(4) mol L(-1) and 2.13 × 10(8) mol L(-1), respectively. At the last step of this procedure, cholic acid level in blood serum and urine which belong to a healthy people were determined by the prepared gold-silver-nanoclusters.
authors.affiliation: Department of Energy Systems Engineering, Karamanoğlu Mehmetbey University, Karaman, Turkey.
authors.forename: Aytaç
authors.initials: A
authors.lastname: Gültekin
authors.forename: Arzu
authors.initials: A
authors.lastname: Ersöz
authors.forename: Adil
authors.initials: A
authors.lastname: Denizli
authors.forename: Rıdvan
authors.initials: R
authors.lastname: Say
mesh_terms: D019826:Cholic Acid / Q000032:analysis / Q000097:blood / Q000737:chemistry* / Q000652:urine; D003545:Cysteine / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D006801:Humans; D008163:Luminescent Measurements; D053768:Metal Nanoparticles / Q000737:chemistry*; D054802:Molecular Imprinting; D058105:Polymerization; D012834:Silver / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry
chemical_list: D013438:Sulfhydryl Compounds; D012834:Silver; D006046:Gold; D019826:Cholic Acid; D003545:Cysteine