title: A tiered approach combining the short time exposure (STE) test and the bovine corneal opacity and permeability (BCOP) assay for predicting eye irritation potential of chemicals.
abstract: For the evaluation of eye irritation, one in vitro alternative test may not completely replace the Draize test. Therefore, a tiered approach combining several in vitro assays, including cytotoxicity assays, is proposed in order to estimate the eye irritation potential of a wide range of chemical classes. The Short Time Exposure (STE) test, a relatively newer alternative eye irritation test, involves exposing Statens seruminstitut rabbit cornea (SIRC) cells for 5 min to two concentrations (5% and 0.05%) of test material. In the present study, we examined the predictive capacity of a tiered approach analyzing the results from the STE test and then the results of the bovine corneal opacity and permeability (BCOP) assay for assessing globally harmonized system (GHS) eye irritation rankings of various chemicals. The accuracy of predicting the GHS rankings was slightly improved when the tiered approach combination of STE test and BCOP assay was used compared to when the STE test irritation rank classification was used alone. Moreover, the under prediction rate was substantially improved when this tiered approach was used. From these results, the tiered approach of combining the data analysis of the STE test and BCOP assay might be a promising alternative eye irritation test strategy.
authors.affiliation: Safety Science Research Laboratories, Kao Corporation, Tochigi, Japan. hayashi.kazuhiko@kao.co.jp
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Hayashi
authors.forename: Taeko
authors.initials: T
authors.lastname: Mori
authors.forename: Takayuki
authors.initials: T
authors.lastname: Abo
authors.forename: Mirei
authors.initials: M
authors.lastname: Koike
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Sakaguchi
authors.forename: Naohiro
authors.initials: N
authors.lastname: Nishiyama
mesh_terms: D000818:Animals; D002417:Cattle; D002460:Cell Line; D003315:Cornea / Q000187:drug effects* / Q000473:pathology; D066298:In Vitro Techniques; D007509:Irritants / Q000633:toxicity*; D010539:Permeability; D011817:Rabbits; D015203:Reproducibility of Results; D023382:Toxicity Tests, Acute / Q000379:methods*
chemical_list: D007509:Irritants