title: QF-PCR: application, overview and review of the literature.
abstract: Quantitative fluorescent polymerase chain reaction has been in diagnostic use in the UK for over 10 years and has proved to be a cost-effective, robust and accurate rapid prenatal test for common aneuploidies. Specific advantages include detection of triploidy, mosaicism and maternal cell contamination. Its application at our centre is described, with developments including stand-alone testing and improvements in strategies for the preparation and testing of chorionic villus biopsies.
authors.affiliation: Cytogenetics Department, GSTS Pathology, Guy's and St Thomas' Hospital, London, UK. kathy.mann@gsts.com
authors.forename: Kathy
authors.initials: K
authors.lastname: Mann
authors.forename: Caroline Mackie
authors.initials: CM
authors.lastname: Ogilvie
mesh_terms: D000328:Adult; D000649:Amniocentesis / Q000379:methods*; D000653:Amniotic Fluid / Q000737:chemistry / Q000166:cytology*; D015193:Chorionic Villi Sampling / Q000379:methods*; D025063:Chromosome Disorders / Q000175:diagnosis* / Q000235:genetics; D005260:Female; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*; D011247:Pregnancy; D015203:Reproducibility of Results