title: Overexpression of triosephosphate isomerase inhibits proliferation of chicken embryonal fibroblast cells.
abstract: Triosephosphate isomerase (TPI), a glycolytic enzyme, functions in catalyzing the interconversion of glyceraldehyde-3-phosphate and dihydroxyacetone phosphate and is generally upregulated in tumours. However, there are data suggesting loss in lymphomas. To determine its effects in chicken embryonal fibroblasts (CEF) a plasmid was constructed to allow transfection. Upon culture in a suitable medium, cells transfected with the TPI demonstrated upregulation and were significantly more susceptible to apoptosis compared to controls with decreased proliferation. These finding therefore render a novel mechanism by which CEF can be triggered to undergo death by upregulation of TPI.
authors.affiliation: Institute of Zoonosis, Jilin University, Changchun, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Dang
authors.forename: Ze
authors.initials: Z
authors.lastname: Wang
authors.forename: Yupei
authors.initials: Y
authors.lastname: Guo
authors.forename: Jianxin
authors.initials: J
authors.lastname: Yang
authors.forename: Zeli
authors.initials: Z
authors.lastname: Xing
authors.forename: Lianzhi
authors.initials: L
authors.lastname: Mu
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zhang
authors.forename: Zhuang
authors.initials: Z
authors.lastname: Ding
mesh_terms: D000818:Animals; D017209:Apoptosis; D049109:Cell Proliferation; D002645:Chickens; D005347:Fibroblasts / Q000201:enzymology* / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D014305:Triose-Phosphate Isomerase / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D012333:RNA, Messenger; D014305:Triose-Phosphate Isomerase