title: Two-dimensional liquid chromatography-tandem mass spectrometry coupled with isobaric tags for relative and absolute quantification (iTRAQ) labeling approach revealed first proteome profiles of pulmonary alveolar macrophages infected with porcine reproductive and respiratory syndrome virus.
abstract: Porcine reproductive and respiratory syndrome (PRRS) has devastated the pig industry worldwide for almost 25 years, and its virus (PRRSV) preferentially infects and replicates in pulmonary alveolar macrophages (PAMs). To discover cellular protein responses in PRRSV-infected PAMs, two-dimensional liquid chromatography-tandem mass spectrometry coupled with isobaric tags for relative and absolute quantification (iTRAQ) labeling was employed to quantitatively identify the differentially expressed proteins between the PRRSV-infected groups and the controls. A total of 160 cellular proteins in PAMs that were significantly altered post-infection were identified. These differentially expressed proteins are related to the biological processes of virus binding, cell structure, signal transduction, cell adhesion, etc., and their interactions. This is the first report that analyzed the cellular protein profile of PRRSV-infected PAMs using iTRAQ technology, and this data provides important information to help understand the host response to PRRSV and to define the cellular requirements for the underlying mechanism of PRRSV replication and pathogenesis.
authors.affiliation: Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Lu
authors.forename: Juan
authors.initials: J
authors.lastname: Bai
authors.forename: Lili
authors.initials: L
authors.lastname: Zhang
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Jiang
authors.forename: Jennifer J
authors.initials: JJ
authors.lastname: Michal
authors.forename: Qindong
authors.initials: Q
authors.lastname: He
authors.forename: Ping
authors.initials: P
authors.lastname: Jiang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002853:Chromatography, Liquid / Q000379:methods*; D005455:Fluorescent Antibody Technique; D054884:Host-Pathogen Interactions; D016676:Macrophages, Alveolar / Q000378:metabolism / Q000821:virology*; D019318:Porcine Reproductive and Respiratory Syndrome / Q000378:metabolism / Q000821:virology; D019316:Porcine respiratory and reproductive syndrome virus / Q000472:pathogenicity* / Q000502:physiology; D060066:Protein Interaction Maps; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D015398:Signal Transduction; D013552:Swine / Q000821:virology; D053719:Tandem Mass Spectrometry / Q000379:methods*; D053585:Virus Attachment; D014779:Virus Replication
chemical_list: D020543:Proteome