title: Tropomodulin 1 constrains fiber cell geometry during elongation and maturation in the lens cortex.
abstract: Lens fiber cells exhibit a high degree of hexagonal packing geometry, determined partly by tropomodulin 1 (Tmod1), which stabilizes the spectrin-actin network on lens fiber cell membranes. To ascertain whether Tmod1 is required during epithelial cell differentiation to fiber cells or during fiber cell elongation and maturation, the authors quantified the extent of fiber cell disorder in the Tmod1-null lens and determined locations of disorder by confocal microscopy and computational image analysis. First, nearest neighbor analysis of fiber cell geometry in Tmod1-null lenses showed that disorder is confined to focal patches. Second, differentiating epithelial cells at the equator aligned into ordered meridional rows in Tmod1-null lenses, with disordered patches first observed in elongating fiber cells. Third, as fiber cells were displaced inward in Tmod1-null lenses, total disordered area increased due to increased sizes (but not numbers) of individual disordered patches. The authors conclude that Tmod1 is required first to coordinate fiber cell shapes and interactions during tip migration and elongation and second to stabilize ordered fiber cell geometry during maturation in the lens cortex. An unstable spectrin-actin network without Tmod1 may result in imbalanced forces along membranes, leading to fiber cell rearrangements during elongation, followed by propagation of disorder as fiber cells mature.
authors.affiliation: The Scripps Research Institute, La Jolla, CA 92037, USA.
authors.forename: Roberta B
authors.initials: RB
authors.lastname: Nowak
authors.forename: Velia M
authors.initials: VM
authors.lastname: Fowler
mesh_terms: D000199:Actins / Q000648:ultrastructure; D000818:Animals; D002452:Cell Count; D002454:Cell Differentiation; D002462:Cell Membrane / Q000648:ultrastructure; D002465:Cell Movement; D048430:Cell Shape; D004847:Epithelial Cells / Q000166:cytology; D007908:Lens, Crystalline / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D051348:Tropomodulin / Q000235:genetics / Q000502:physiology*
chemical_list: D000199:Actins; C495831:Tmod1 protein, mouse; D051348:Tropomodulin