title: Biocontrol of Penicillium nordicum growth and ochratoxin A production by native yeasts of dry cured ham.
abstract: Twelve yeast strains isolated from the surface of Italian typical dry-cured hams, belonging to D. hansenii, D. maramus, C. famata, C. zeylanoides and H. burtonii species, and previously selected for their ability to grow in dry-cured ham-like substrates, were screened for antagonistic activity against a toxigenic strain of P. nordicum and inhibition of ochratoxin A (OTA) biosynthesis. On average, yeast inhibitory activity was lowered by increasing fungal inoculum and enhanced by NaCl presence. In the assay conditions, H. burtonii and C. zeylanoides were the most effective, both in inhibiting P. nordicum growth and OTA production. D. hansenii was the species with the lowest inhibitory activity, especially in the absence of salt. OTA production dropped from the range < LOD - 5000 ppb in P. nordicum control plates to the range < LOD - 200 ppb in yeast-added plates. OTA production increased in the presence of NaCl in P. nordicum control plates, while salt enhanced inhibition against OTA production in yeast-added plates.
authors.affiliation: Stazione Sperimentale per l'Industria delle Conserve Alimentari, V.le F. Tanara, 31/A, Parma 43121, Italy. roberta.virgili@ssica.it
authors.forename: Roberta
authors.initials: R
authors.lastname: Virgili
authors.forename: Nicoletta
authors.initials: N
authors.lastname: Simoncini
authors.forename: Tania
authors.initials: T
authors.lastname: Toscani
authors.forename: Marco
authors.initials: M
authors.lastname: Camardo Leggieri
authors.forename: Silvia
authors.initials: S
authors.lastname: Formenti
authors.forename: Paola
authors.initials: P
authors.lastname: Battilani
mesh_terms: D000818:Animals; D000898:Antibiosis / Q000502:physiology*; D001688:Biological Products; D005506:Food Contamination; D005516:Food Microbiology; D005519:Food Preservation / Q000379:methods; D005656:Fungal Proteins / Q000378:metabolism* / Q000494:pharmacology; D008461:Meat Products / Q000382:microbiology*; D009793:Ochratoxins / Q000096:biosynthesis*; D010407:Penicillium / Q000254:growth & development* / Q000378:metabolism; D013552:Swine; D015003:Yeasts / Q000378:metabolism* / Q000472:pathogenicity
chemical_list: D001688:Biological Products; D005656:Fungal Proteins; D009793:Ochratoxins; C025589:ochratoxin A