title: Preparation of miniantibodies to Azospirillum brasilense Sp245 surface antigens and their use for bacterial detection.
abstract: This article reports the first preparation of miniantibodies to Azospirillum brasilense Sp245 surface antigens by using a combinatorial phage library of sheep antibodies. The prepared phage antibodies were used for the first time for lipopolysaccharide and flagellin detection by dot assay, electro-optical analysis of cell suspensions, and transmission electron microscopy. Interaction of A. brasilense Sp245 with antilipopolysaccharide and antiflagellin phage-displayed miniantibodies caused the magnitude of the electro-optical signal to change considerably. The electro-optical results were in good agreement with the electron microscopic data. This is the first reported possibility of employing phage-displayed miniantibodies in bacterial detection aided by electro-optical analysis of cell suspensions.
authors.affiliation: Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov, Russian Federation.
authors.forename: Lev A
authors.initials: LA
authors.lastname: Dykman
authors.forename: Sergei A
authors.initials: SA
authors.lastname: Staroverov
authors.forename: Olga I
authors.initials: OI
authors.lastname: Guliy
authors.forename: Oleg V
authors.initials: OV
authors.lastname: Ignatov
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Fomin
authors.forename: Irina V
authors.initials: IV
authors.lastname: Vidyasheva
authors.forename: Olga A
authors.initials: OA
authors.lastname: Karavaeva
authors.forename: Viktor D
authors.initials: VD
authors.lastname: Bunin
authors.forename: Gennady L
authors.initials: GL
authors.lastname: Burygin
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000737:chemistry / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology*; D015806:Azospirillum brasilense / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification*; D020650:Combinatorial Chemistry Techniques; D004563:Electrochemistry; D004926:Escherichia coli / Q000276:immunology; D005408:Flagellin / Q000032:analysis / Q000276:immunology; D015151:Immunoblotting; D008070:Lipopolysaccharides / Q000032:analysis / Q000276:immunology; D046529:Microscopy, Electron, Transmission; D012756:Sheep
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D000954:Antigens, Surface; D008070:Lipopolysaccharides; D005408:Flagellin