title: Vibrio inhibens sp. nov., a novel bacterium with inhibitory activity against Vibrio species.
abstract: Strain BFLP-10(T), isolated from faeces of wild long-snouted seahorses (Hippocampus guttulatus), is a Gram-negative, motile and facultatively anaerobic rod. This bacterium produces inhibitory activity against Vibrio species. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BFLP-10(T) was a member of the genus Vibrio and was most closely related to Vibrio owensii (99%), Vibrio communis (98.9%), Vibrio sagamiensis (98.9%) and Vibrio rotiferianus (98.4%). However, multilocus sequence analysis using gyrB, pyrH, recA and topA genes revealed low levels of sequence similarity (<91.2%) with these closely related species. In addition, strain BFLP-10(T) could be readily differentiated from other closely related species by several phenotypic properties and fatty acid profiles. The G+C content of the DNA was 45.6 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain BFLP-10(T) represents a novel species within the genus Vibrio, for which the name Vibrio inhibens sp. nov. is proposed. The type strain is BFLP-10(T) (=CECT 7692(T)=DSM 23440(T)).
authors.affiliation: Instituto de Investigaciones Marinas, Consejo Superior de Investigaciones Científicas-IIM-CSIC, c/. Eduardo Cabello 6, Vigo, Spain. jlbalcazar@icra.cat
authors.forename: José Luis
authors.initials: JL
authors.lastname: Balcázar
authors.forename: Miquel
authors.initials: M
authors.lastname: Planas
authors.forename: José
authors.initials: J
authors.lastname: Pintado
mesh_terms: D000818:Animals; D001483:Base Sequence; D027081:DNA Gyrase / Q000737:chemistry / Q000235:genetics; D004264:DNA Topoisomerases, Type I / Q000737:chemistry / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009703:Nucleoside-Phosphate Kinase / Q000737:chemistry / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics; D011938:Rec A Recombinases / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment; D023701:Smegmamorpha / Q000382:microbiology*; D014733:Vibrio / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000648:ultrastructure
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; C023276:uridine monophosphate kinase; D009703:Nucleoside-Phosphate Kinase; D011938:Rec A Recombinases; D004264:DNA Topoisomerases, Type I; D027081:DNA Gyrase