title: Eliminating the scattering ambiguity in multifocal, multimodal, multiphoton imaging systems.
abstract: In this work we present how to entirely remove the scattering ambiguity present in existing multiphoton multifocal systems. This is achieved through the development and implementation of single-element detection systems that incorporate high-speed photon-counting electronics. These systems can be used to image entire volumes in the time it takes to perform a single transverse scan (four depths simultaneously at a rate of 30 Hz). In addition, this capability is further exploited to accomplish single-element detection of multiple modalities (two photon excited fluorescence and second harmonic generation) and to perform efficient image deconvolution. Finally, we demonstrate a new system that promises to significantly simplify this promising technology.
authors.affiliation: Center for Microintegrated Optics for Advanced Bioimaging and Control, and Department of Physics, Colorado School of Mines, Golden, Colorado 80401, USA. ehoover@mines.edu
authors.forename: Erich E
authors.initials: EE
authors.lastname: Hoover
authors.forename: Jeffrey J
authors.initials: JJ
authors.lastname: Field
authors.forename: David G
authors.initials: DG
authors.lastname: Winters
authors.forename: Michael D
authors.initials: MD
authors.lastname: Young
authors.forename: Eric V
authors.initials: EV
authors.lastname: Chandler
authors.forename: John C
authors.initials: JC
authors.lastname: Speirs
authors.forename: Jacob T
authors.initials: JT
authors.lastname: Lapenna
authors.forename: Susy M
authors.initials: SM
authors.lastname: Kim
authors.forename: Shi-You
authors.initials: SY
authors.lastname: Ding
authors.forename: Randy A
authors.initials: RA
authors.lastname: Bartels
authors.forename: Jing W
authors.initials: JW
authors.lastname: Wang
authors.forename: Jeff A
authors.initials: JA
authors.lastname: Squier
mesh_terms: D000818:Animals; D002482:Cellulose / Q000378:metabolism; D004331:Drosophila melanogaster / Q000166:cytology; D007091:Image Processing, Computer-Assisted; D008164:Luminescent Proteins / Q000378:metabolism; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*; D012542:Scattering, Radiation; D013213:Starch / Q000737:chemistry; D003313:Zea mays / Q000737:chemistry; D000097573:Red Fluorescent Protein
chemical_list: D002482:Cellulose; D008164:Luminescent Proteins; D013213:Starch; D000097573:Red Fluorescent Protein