title: Fabrication of functional three-dimensional tissues by stacking cell sheets in vitro.
abstract: The fabrication of 3D tissues retaining the original functions of tissues/organs in vitro is crucial for optimal tissue engineering and regenerative medicine. The fabrication of 3D tissues also contributes to the establishment of in vitro tissue/organ models for drug screening. Our laboratory has developed a fabrication system for functional 3D tissues by stacking cell sheets of confluent cultured cells detached from a temperature-responsive culture dish. Here we describe the protocols for the fabrication of 3D tissues by cell sheet engineering. Three-dimensional cardiac tissues fabricated by stacking cardiac cell sheets pulsate spontaneously, synchronously and macroscopically. Via this protocol, it is also possible to fabricate other tissues, such as 3D tissue including capillary-like prevascular networks, from endothelial cells sandwiched between layered cell sheets. Cell sheet stacking technology promises to provide in vitro tissue/organ models and more effective therapies for curing tissue/organ failures.
authors.affiliation: Institute of Advanced Biomedical Engineering and Science, TWIns, Tokyo Women's Medical University, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Haraguchi
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Shimizu
authors.forename: Tadashi
authors.initials: T
authors.lastname: Sasagawa
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Sekine
authors.forename: Katsuhisa
authors.initials: K
authors.lastname: Sakaguchi
authors.forename: Tetsutaro
authors.initials: T
authors.lastname: Kikuchi
authors.forename: Waki
authors.initials: W
authors.lastname: Sekine
authors.forename: Sachiko
authors.initials: S
authors.lastname: Sekiya
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yamato
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Umezu
authors.forename: Teruo
authors.initials: T
authors.lastname: Okano
mesh_terms: D000200:Action Potentials; D000818:Animals; D048091:Guided Tissue Regeneration; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D032448:Myoblasts, Skeletal; D009206:Myocardium / Q000166:cytology; D051381:Rats; D017208:Rats, Wistar; D046509:Tissue Culture Techniques; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D020136:Hydrogel, Polyethylene Glycol Dimethacrylate