title: Flexible plastic bioreactors for photobiological hydrogen production by hydrogenase-deficient cyanobacteria.
abstract: Uptake hydrogenase mutant cells of the cyanobacterium Nostoc sp. PCC 7422 photobiologically produced H(2) catalyzed by nitrogenase for several days in H(2)-barrier transparent plastic bags, and accumulated H(2) in the presence of O(2) evolved by photosynthesis. Their H(2) production activity was higher in the sealed flexible bags than in stoppered serum bottles of fixed gas volume.
authors.affiliation: Department of Biological Sciences, Kanagawa University, Hiratsuka, Kanagawa, Japan.
authors.forename: Masaharu
authors.initials: M
authors.lastname: Kitashima
authors.forename: Hajime
authors.initials: H
authors.lastname: Masukawa
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Sakurai
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Inoue
mesh_terms: D000332:Aerobiosis; D019149:Bioreactors; D000458:Cyanobacteria / Q000378:metabolism*; D017353:Gene Deletion; D006859:Hydrogen / Q000378:metabolism*; D006864:Hydrogenase / Q000235:genetics; D008567:Membranes, Artificial; D010100:Oxygen / Q000378:metabolism; D010788:Photosynthesis / Q000502:physiology*; D010969:Plastics; D018583:Pliability
chemical_list: D008567:Membranes, Artificial; D010969:Plastics; D006859:Hydrogen; D006864:Hydrogenase; D010100:Oxygen