title: Dendritic cell subsets in neoplastic tissue and peripheral blood of             laryngeal cancer patients: relation with grade and stage of the disease.
abstract: Despite improvements in immunotherapy, little is known about dendritic cell             (DC) subpopulations naturally occurring in the laryngeal cancer tissue (LCT) and             peripheral blood (PB) of untreated laryngeal cancer (LC) patients. The purpose             of the present study was to evaluate mature, immature myeloid and plasmacytoid             DCs in the LCT and PB of patients with various stages and grades of squamous cell             carcinoma of the larynx (n=66) and PB of healthy donors (n=20), and to explore             the correlation of the percentages of the DCs to clinical parameters. The percentage             of DCs in LCT and PB was determined using monoclonal antibodies and flow cytometry.             It has been revealed that DCs accumulate in LCT in comparison with their content             in PB. The myeloid to lymphoid/plasmacytoid DC ratio was higher in LCT compared             to PB. It was found that in cases of poorly-differentiated LC, there were higher             percentages of lymphoid/plasmacytoid DCs in LCT in comparison to their content             in the PB. Moreover, in the blood of patients with T4 cancers we found significantly             lower percentages of myeloid DCs in comparison to individuals with T1 neoplasms.             The percentage of myeloid DCs infiltrating cancer tissue positively correlated             with the T stage. In patients with no metastases in the lymph nodes, PB contained             less mature DCs but higher amount of myeloid DCs compared to LCT. Alteration of             the DC proportion in LC patients may result in the development of immunotolerance.
authors.affiliation: Department of Otolaryngology and Laryngeal Oncology, Medical                     University of Lublin, Lublin, Poland.
authors.forename: Janusz
authors.initials: J
authors.lastname: Klatka
authors.forename: Ewelina
authors.initials: E
authors.lastname: Grywalska
authors.forename: Jacek
authors.initials: J
authors.lastname: Tabarkiewicz
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Polak
authors.forename: Jacek
authors.initials: J
authors.lastname: Rolinski
mesh_terms: D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000378:metabolism; D000943:Antigens, Differentiation / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000097:blood / Q000276:immunology / Q000473:pathology*; D016022:Case-Control Studies; D002452:Cell Count; D003713:Dendritic Cells / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007822:Laryngeal Neoplasms / Q000097:blood / Q000276:immunology / Q000473:pathology*; D007963:Leukocytes, Mononuclear / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging
chemical_list: D015703:Antigens, CD; D000943:Antigens, Differentiation