title: Lysyl oxidase-like 2 deaminates lysine 4 in histone H3.
abstract: Methylation of lysine 4 (K4) within histone H3 has been linked to active transcription and is removed by LSD1 and the JmjC domain-containing proteins by amino-oxidation or hydroxylation, respectively. Here, we describe the deamination catalyzed by Lysyl oxidase-like 2 protein (LOXL2) as an unconventional chemical mechanism for H3K4 modification. Infrared spectroscopy and mass spectrometry analyses demonstrated that recombinant LOXL2 specifically deaminates trimethylated H3K4. Moreover, LOXL2 activity is linked with the transcriptional control of CDH1 gene by regulating H3K4me3 deamination. These results reveal another H3 modification and provide a different mechanism for H3K4 modification.
authors.affiliation: Programa de Recerca en Càncer, IMIM-Hospital del Mar, Barcelona, Spain.
authors.forename: Nicolás
authors.initials: N
authors.lastname: Herranz
authors.forename: Natàlia
authors.initials: N
authors.lastname: Dave
authors.forename: Alba
authors.initials: A
authors.lastname: Millanes-Romero
authors.forename: Lluis
authors.initials: L
authors.lastname: Morey
authors.forename: Víctor M
authors.initials: VM
authors.lastname: Díaz
authors.forename: Víctor
authors.initials: V
authors.lastname: Lórenz-Fonfría
authors.forename: Ricardo
authors.initials: R
authors.lastname: Gutierrez-Gallego
authors.forename: Celia
authors.initials: C
authors.lastname: Jerónimo
authors.forename: Luciano
authors.initials: L
authors.lastname: Di Croce
authors.forename: Antonio
authors.initials: A
authors.lastname: García de Herreros
authors.forename: Sandra
authors.initials: S
authors.lastname: Peiró
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000502:physiology*; D015703:Antigens, CD; D015820:Cadherins / Q000235:genetics; D045744:Cell Line, Tumor; D003641:Deamination; D005786:Gene Expression Regulation; D006657:Histones / Q000378:metabolism*; D006801:Humans; D008239:Lysine / Q000378:metabolism; D008745:Methylation
chemical_list: D015703:Antigens, CD; C513384:CDH1 protein, human; D015820:Cadherins; D006657:Histones; D000594:Amino Acid Oxidoreductases; C119091:LOXL2 protein, human; D008239:Lysine