title: Sin3 interacts with Foxk1 and regulates myogenic progenitors.
abstract: We have previously reported Foxk1 as an important transcription factor in the myogenic progenitors. SWI-independent-3 (Sin3) has been identified as a Foxk1 binding candidate using a yeast two-hybrid screen. In the present study, we have identified the Foxk1 N-terminal (1-40) region as the Sin3 interacting domain (SID), and the PAH2 of Sin3 as the Foxk1 binding domain utilizing yeast two-hybrid and GST pull-down assays. Further studies revealed that knockdown of Sin3a or Sin3b results in cell cycle arrest and upregulation of cell cycle inhibitor genes. In summary, our present studies have shown that Foxk1 interacts with Sin3 through the SID and that Sin3 has an important role in the regulation of cell cycle kinetics of the MPC population. The results of these studies continue to define and assemble the networks that regulate the MPCs and muscle regeneration.
authors.affiliation: Lillehei Heart Institute, University of Minnesota-Twin Cities, 4-108 NHH, 312 Church St SE, Minneapolis, MN 55455, USA.
authors.forename: Xiaozhong
authors.initials: X
authors.lastname: Shi
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Garry
mesh_terms: D000818:Animals; D059447:Cell Cycle Checkpoints; D002460:Cell Line; D051858:Forkhead Transcription Factors / Q000737:chemistry / Q000378:metabolism*; D020869:Gene Expression Profiling; D055785:Gene Knockdown Techniques; D051379:Mice; D010446:Peptide Fragments / Q000378:metabolism; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D034622:RNA Interference; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D032496:Satellite Cells, Skeletal Muscle / Q000378:metabolism*; D056264:Sin3 Histone Deacetylase and Corepressor Complex; D020798:Two-Hybrid System Techniques
chemical_list: D051858:Forkhead Transcription Factors; C497672:Foxk1 protein, mouse; D010446:Peptide Fragments; D012097:Repressor Proteins; C099683:SIN3A transcription factor; C115812:Sin3b protein, mouse; D056264:Sin3 Histone Deacetylase and Corepressor Complex