title: Radical scavenging activity of spring mountain herbs in the Shikoku mountain area and identification of antiradical constituents by simple HPLC detection and LC-MS methods.
abstract: The functionality of spring mountain herbs, which were collected in the Kajigamori mountain area of Shikoku area in Japan, was investigated in the course of our studies for utilizing local plant resources. The radical scavenging activity of the extracts from seventeen herbs was measured. Among these herbs, two extracts from Polystichym ovato-paleaceum (Japanese name: Tsuyanashiinode) and Sambucus racemosa subsp. sieboldiana (Japanese name: Niwatoko) showed potent DPPH radical scavenging activity. The material evidence for the potent activity of the extracts was studied by a combination of our developed method for detecting antiradical compounds, LC-MS/MS, and enzymatic hydrolysis.
authors.affiliation: Graduate School of Integrated Arts and Sciences, University of Tokushima, Tokushima, Japan. masuda@ias.tokushima-u.ac.jp
authors.forename: Toshiya
authors.initials: T
authors.lastname: Masuda
authors.forename: Tomoko
authors.initials: T
authors.lastname: Inouchi
authors.forename: Aya
authors.initials: A
authors.lastname: Fujimoto
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Shingai
authors.forename: Miyuki
authors.initials: M
authors.lastname: Inai
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Nakamura
authors.forename: Shoji
authors.initials: S
authors.lastname: Imai
mesh_terms: D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors; D002109:Caffeic Acids / Q000032:analysis; D002853:Chromatography, Liquid; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification*; D005609:Free Radicals / Q000037:antagonists & inhibitors*; D007564:Japan; D044949:Kaempferols / Q000032:analysis; D060985:Natural Springs; D010851:Picrates / Q000037:antagonists & inhibitors; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D032493:Polystichum / Q000737:chemistry*; D011794:Quercetin / Q000031:analogs & derivatives / Q000032:analysis; D027923:Sambucus / Q000737:chemistry*; D053719:Tandem Mass Spectrometry
chemical_list: D001713:Biphenyl Compounds; D002109:Caffeic Acids; D016166:Free Radical Scavengers; D005609:Free Radicals; D044949:Kaempferols; D010851:Picrates; D010936:Plant Extracts; C006552:kaempferol; D011794:Quercetin; C004931:1,1-diphenyl-2-picrylhydrazyl; C040048:caffeic acid