title: Silver nanoparticles modify VEGF signaling pathway and mucus hypersecretion in allergic airway inflammation.
abstract: The anti-inflammatory action of silver nanoparticles (NPs) has been reported in a murine model of asthma in a previous study. But more specific mechanisms of silver NPs in an attenuation of allergic airway inflammation have not yet been established. Vascular and mucous changes are believed to contribute largely in pathophysiology in asthma. Among various factors related to vascular changes, vascular endothelial growth factor (VEGF) plays a pivotal role in vascular changes in asthma. Mucin proteins MUC5AC and MUC5B have been implicated as markers of goblet cell metaplasia in lung pathologies. The aim of this study was to investigate the effects of silver NPs on VEGF signaling pathways and mucus hypersecretion. Ovalbumin (OVA)-inhaled female BALBc mice were used to evaluate the role of silver NPs and the related molecular mechanisms in allergic airway disease. In this study, with an OVA-induced murine model of allergic airway disease, it was found that the increased levels of hypoxia-inducible factor (HIF)-1α, VEGF, phosphatidylinositol-3 kinase (PI3K) and phosphorylated-Akt levels, and mucous glycoprotein expression (Muc5ac) in lung tissues were substantially decreased by the administration of silver NPs. In summary, silver NPs substantially suppressed mucus hypersecretion and PI3K/HIF-1α/VEGF signaling pathway in an allergic airway inflammation.
authors.affiliation: Division of Pulmonology, Allergy and Critical Care Medicine, Department of Internal Medicine, Chungnam National University Medical School, Daesadong, Daejeon, South Korea.
authors.forename: Sunhyae
authors.initials: S
authors.lastname: Jang
authors.forename: Ji Won
authors.initials: JW
authors.lastname: Park
authors.forename: Hye Rim
authors.initials: HR
authors.lastname: Cha
authors.forename: Sun Young
authors.initials: SY
authors.lastname: Jung
authors.forename: Jeong Eun
authors.initials: JE
authors.lastname: Lee
authors.forename: Sung Soo
authors.initials: SS
authors.lastname: Jung
authors.forename: Ju Ock
authors.initials: JO
authors.lastname: Kim
authors.forename: Sun Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Choong Sik
authors.initials: CS
authors.lastname: Lee
authors.forename: Hee Sun
authors.initials: HS
authors.lastname: Park
mesh_terms: D000818:Animals; D001980:Bronchi / Q000187:drug effects / Q000473:pathology; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D004305:Dose-Response Relationship, Drug; D005260:Female; D006651:Histocytochemistry; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism; D007378:Interleukins / Q000378:metabolism; D008168:Lung / Q000187:drug effects / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D055271:Mucin 5AC / Q000378:metabolism; D009093:Mucus / Q000378:metabolism*; D011014:Pneumonia / Q000139:chemically induced / Q000378:metabolism* / Q000503:physiopathology; D015398:Signal Transduction / Q000187:drug effects*; D012834:Silver / Q000737:chemistry / Q000494:pharmacology*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D007378:Interleukins; C524679:Muc5ac protein, mouse; D055271:Mucin 5AC; D042461:Vascular Endothelial Growth Factor A; D012834:Silver