title: Whole-cell based solvent-free system for one-pot production of biodiesel from waste grease.
abstract: A whole-cell based solvent-free system was developed for efficient conversion of waste grease to biodiesel via one-pot esterification and transesterification. By isolation and screening of lipase-producing strains from soil, Serratia marcescens YXJ-1002 was discovered for the biotransformation of grease to biodiesel. The lipase (SML) from this strain was cloned and expressed in Escherichia coli as an intracellular enzyme, showing 6 times higher whole-cell based hydrolysis activity than that of wild type strain. The recombinant cells were used for biodiesel production from waste grease in one-pot reactions containing no solvent with the addition of methanol in several small portions, and 97% yield of biodiesel (FAME) was achieved under optimized conditions. In addition, the whole-cell biocatalysts showed excellent reusability, retaining 74% productivity after 4 cycles. The developed system, biocatalyst, and process enable the efficient, low-cost, and green production of biodiesel from waste grease, providing with a potential industrial application.
authors.affiliation: Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117576, Singapore.
authors.forename: Aitao
authors.initials: A
authors.lastname: Li
authors.forename: Thao P N
authors.initials: TP
authors.lastname: Ngo
authors.forename: Jinyong
authors.initials: J
authors.lastname: Yan
authors.forename: Kaiyuan
authors.initials: K
authors.lastname: Tian
authors.forename: Zhi
authors.initials: Z
authors.lastname: Li
mesh_terms: D001673:Biodegradation, Environmental; D056804:Biofuels / Q000382:microbiology*; D005223:Fats / Q000378:metabolism*; D007220:Industrial Waste / Q000517:prevention & control*; D012706:Serratia marcescens / Q000378:metabolism*; D012997:Solvents
chemical_list: D056804:Biofuels; D005223:Fats; D007220:Industrial Waste; D012997:Solvents