title: Soil fungal cellobiohydrolase I gene (cbhI) composition and expression in a loblolly pine plantation under conditions of elevated atmospheric CO2 and nitrogen fertilization.
abstract: The simultaneous increase of atmospheric CO(2) and nitrogen (N) deposition to terrestrial ecosystems is predicted to alter plant productivity and, consequently, to change the amount and quality of above- and belowground carbon entering forest soils. It is not known how such changes will impact the composition and function of soil fungal communities that play a key role in degrading complex carbon. We sequenced the fungal cellobiohydrolase I gene (cbhI) from soil DNA and cDNA to compare the richness and composition of resident and expressed cbhI genes at a U.S. Department of Energy free air-carbon dioxide enrichment (FACE) site (NC), which had been exposed to elevated atmospheric CO(2) and/or N fertilization treatment for several years. Our results provide evidence that the richness and composition of the cellulolytic fungi surveyed in this study were distinct in the DNA- and cDNA-based gene surveys and were dominated by Basidiomycota that have low or no representation in public databases. The surveys did not detect differences in richness or phylum-level composition of cbhI-containing, cellulolytic fungi that correlated with elevated CO(2) or N fertilization at the time of sampling.
authors.affiliation: Los Alamos National Laboratory, Los Alamos, NM, USA.
authors.forename: Carolyn F
authors.initials: CF
authors.lastname: Weber
authors.forename: Monica Moya
authors.initials: MM
authors.lastname: Balasch
authors.forename: Zachary
authors.initials: Z
authors.lastname: Gossage
authors.forename: Andrea
authors.initials: A
authors.lastname: Porras-Alfaro
authors.forename: Cheryl R
authors.initials: CR
authors.lastname: Kuske
mesh_terms: D001487:Basidiomycota / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism*; D043366:Cellulose 1,4-beta-Cellobiosidase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D021903:DNA, Ribosomal Spacer / Q000032:analysis / Q000235:genetics; D017753:Ecosystem; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000378:metabolism*; D010802:Phylogeny; D041603:Pinus taeda / Q000254:growth & development*; D017422:Sequence Analysis, DNA; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology; D014197:Trees
chemical_list: D021903:DNA, Ribosomal Spacer; D005656:Fungal Proteins; D012987:Soil; D002245:Carbon Dioxide; D043366:Cellulose 1,4-beta-Cellobiosidase; D009584:Nitrogen