title: Liquid chromatographic determination of quinolones in water and human urine samples after microextraction by packed sorbent.
abstract: A method for the simultaneous determination of quinolones in water and urine samples by microextraction in a sorbent-packed syringe (MEPS) with LC is described. MEPS is a new miniaturized SPE technique that can be used with chromatographic instruments without any modifications. In MEPS, approximately 1 mg of the solid packing material is inserted into a syringe (100-250 microL) as a plug. Sample preparation takes place on the packed bed. The new method is promising, easy to use, economical, and rapid. The determination of quinolones in groundwater and urine was performed using MEPS as a sample preparation method with LC-UV determination. Four quinolone antibiotics--enrofloxacin, enoxacin, danofloxacin, and nalidixic acid--in groundwater and urine samples were used as analytes. The extraction recovery was found to be between 64.9 and 98.9%. The results showed high correlation coefficients (R2 > 0.992) for all of the analytes within the calibration range. The LOQ was between 0.091 and 0.315 ng/mL.
authors.affiliation: Punjabi University, Department of Chemistry, Patiala 147 002, India.
authors.forename: Susheela
authors.initials: S
authors.lastname: Rani
authors.forename: Ashwini
authors.initials: A
authors.lastname: Kumar
authors.forename: Ashok Kumar
authors.initials: AK
authors.lastname: Malik
authors.forename: Baldev
authors.initials: B
authors.lastname: Singh
mesh_terms: D000327:Adsorption; D000465:Algorithms; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D060587:Groundwater / Q000032:analysis*; D006801:Humans; D007194:India; D007202:Indicators and Reagents; D059627:Liquid Phase Microextraction / Q000379:methods; D015363:Quinolones / Q000032:analysis* / Q000652:urine*; D012015:Reference Standards; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
chemical_list: D007202:Indicators and Reagents; D015363:Quinolones