title: Comparison of immunomodulatory effects of placenta mesenchymal stem cells with bone marrow and adipose mesenchymal stem cells.
abstract: Mesenchymal stem cells (MSCs) are powerful sources for cell therapy in regenerative medicine because they can be isolated from various tissues, expanded, and induced into multiple-lineages. Of note, their immunomodulatory effects maximize the therapeutic effects of stem cells engrafted on host, making them an especially attractive choice. Recently, several varieties of placenta-derived stem cells (PDSCs) including chorionic plate-derived MSCs (CP-MSCs) have been suggested as alternative sources of stem cells. However, comparative studies of immunomodulatory effects for CP-MSCs among various MSCs are largely lacking. We examined and compared immunomodulatory function of CP-MSCs with that of BM-MSCs and AD-MSCs using co-culture system with activated T-cells derived from human umbilical cord blood (UCB) exposed to anti-CD3 and anti-CD28 which are T-cell activating monoclonal antibodies. All MSCs expressed markers of stem cells and three germ layers by RT-PCR. These cells also exhibited comparable immunomodulatory effects when they were co-cultured with activated T-cells in dose-dependent manner. However, expression of HLA-ABC and HLA-G was highly positive in CP-MSCs compared to other MSCs, and higher levels of cytokines of IL-2, IL-4, IL-13, and GM-CSF were detected in dose-dependent manner in CP-MSCs. Taken together, the results of the present study suggest that while CP-MSCs, BM-MSCs, and AD-MSCs all have immunomodulatory effects, CP-MSCs may have additional advantage over the other MSCs in terms of immunomodulation. In conjunction with other previous studies, CP-MSCs are suggested to be a useful stem cell source in cell therapy.
authors.affiliation: Department of Internal Medicine, CHA Bundang Medical Center, CHA University, 351 Yatap-dong, Bundang-gu, Seongnam 463-712, Republic of Korea.
authors.forename: Jung Min
authors.initials: JM
authors.lastname: Lee
authors.forename: Jieun
authors.initials: J
authors.lastname: Jung
authors.forename: Hyun-Jung
authors.initials: HJ
authors.lastname: Lee
authors.forename: Su Jin
authors.initials: SJ
authors.lastname: Jeong
authors.forename: Kyung Jin
authors.initials: KJ
authors.lastname: Cho
authors.forename: Seong-Gyu
authors.initials: SG
authors.lastname: Hwang
authors.forename: Gi Jin
authors.initials: GJ
authors.lastname: Kim
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000276:immunology*; D002823:Chorion / Q000166:cytology / Q000276:immunology; D018920:Coculture Techniques; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D005260:Female; D005312:Fetal Blood / Q000166:cytology / Q000276:immunology; D015870:Gene Expression; D005819:Genetic Markers / Q000276:immunology; D006680:HLA Antigens / Q000235:genetics / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology*; D006801:Humans; D056747:Immunomodulation; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000276:immunology*; D010920:Placenta / Q000166:cytology* / Q000276:immunology*; D011247:Pregnancy; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology
chemical_list: D016207:Cytokines; D005819:Genetic Markers; D006680:HLA Antigens