title: Associations between sperm abnormalities, breed, age, and scrotal circumference in beef bulls.
abstract: The objectives of this study were to determine the associations of breed, age, and scrotal circumference (SC), and their interaction, on the prevalence of sperm abnormalities in beef bulls in Alberta, Canada, and the percentage of satisfactory potential breeders identified during breeding soundness examination solely due to normal sperm morphology. Eosin-nigrosin stained semen smears and evaluation reports of 1642 bull breeding soundness evaluations were procured from 6 veterinary clinics in Alberta. Sperm morphology was determined for at least 100 sperm per bull. The most common defects were detached head [4.86% ± 5.71%; mean ± standard deviation (s)], distal midpiece reflex (6.19% ± 9.13%), and bent tail (1.01% ± 1.54%). Although breed, age, and SC did not significantly affect the prevalence of head or midpiece defects, morphologically normal or abnormal sperm, tail defects were more prevalent in Angus and Hereford bulls compared with other breeds. Overall, solely on the basis of sperm morphology, 1363 (83.0%) bulls were classified as satisfactory potential breeders and the remainder 279 (17.0%) as unsatisfactory (> 30% abnormal sperm, > 20% defective heads, or both). Although not significantly different, the breed with the highest percentage of satisfactory potential breeders was Limousin (90.6%) and the lowest was Hereford (78.8%). That 17% of bulls subjected to breeding soundness evaluation were designated as unsatisfactory solely on the basis of sperm morphology highlights its importance.
authors.affiliation: Department of Production Animal Health, University of Calgary, Calgary, Alberta.
authors.forename: Ajitkumar G
authors.initials: AG
authors.lastname: Menon
authors.forename: Herman W
authors.initials: HW
authors.lastname: Barkema
authors.forename: Randy
authors.initials: R
authors.lastname: Wilde
authors.forename: John P
authors.initials: JP
authors.lastname: Kastelic
authors.forename: Jacob C
authors.initials: JC
authors.lastname: Thundathil
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D002417:Cattle / Q000033:anatomy & histology / Q000235:genetics* / Q000502:physiology*; D008297:Male; D012611:Scrotum / Q000033:anatomy & histology*; D055101:Semen Analysis / Q000662:veterinary; D013094:Spermatozoa / Q000002:abnormalities* / Q000166:cytology