title: Application of magnetic hydroxyapatite nanoparticles for solid phase extraction of plasmid DNA.
abstract: We developed a facile method for plasmid DNA (pDNA) extraction from crude Escherichia coli lysate using magnetic hydroxyapatite nanoparticles (MHapNPs) in the presence of polyethylene glycol (PEG)/NaCl. DNA condensation induced by PEG/NaCl is a prerequisite for achieving pronounced DNA recovery. The quality and quantity of MHapNP-purified pDNA under optimal binding buffer conditions (0.5 volume of 20% PEG 8000/2M NaCl) were comparable to those obtained using organic solvents or commercial kits. This MHapNP technique is rapid, simple, cost-effective, and environmentally friendly and has the potential to extract DNA from other cell lysates.
authors.affiliation: Faculty of Science, Sichuan Agricultural University, Yaan 625014, China. mesopig@hotmail.com
authors.forename: Zhi
authors.initials: Z
authors.lastname: Shan
authors.forename: Xianghai
authors.initials: X
authors.lastname: Li
authors.forename: Yaying
authors.initials: Y
authors.lastname: Gao
authors.forename: Xianxiang
authors.initials: X
authors.lastname: Wang
authors.forename: Chenglei
authors.initials: C
authors.lastname: Li
authors.forename: Qi
authors.initials: Q
authors.lastname: Wu
mesh_terms: D004247:DNA / Q000032:analysis* / Q000302:isolation & purification; D017886:Durapatite / Q000737:chemistry*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D008280:Magnetics; D053758:Nanoparticles / Q000737:chemistry*; D010957:Plasmids / Q000235:genetics; D011092:Polyethylene Glycols / Q000737:chemistry; D012965:Sodium Chloride / Q000737:chemistry; D052616:Solid Phase Extraction
chemical_list: D011092:Polyethylene Glycols; D012965:Sodium Chloride; D004247:DNA; D017886:Durapatite