title: Hemopexin is upregulated in rat intestinal mucosa injured by indomethacin.
abstract: OBJECTIVE
  Recent advancements in capsule endoscopy and double-balloon endoscopy have revealed that non-steroidal anti-inflammatory drugs (NSAIDs), such as indomethacin, can induce small intestinal mucosal damage. However, the precise pathogenesis and therapeutic strategy have not been fully revealed. The aim of the present study was to determine the upregulated proteins in the small intestine exposed to indomethacin.
  
  
  METHODS
  Indomethacin (10 mg/kg) was administered subcutaneously to male Wistar rats to induce small intestinal damage and the severity of the intestinal injury was evaluated by measuring the area of visible ulcerative lesions. The intestinal mucosal tissue samples were collected and then analyzed by two-dimensional gel electrophoresis, with matrix-assisted laser desorption/ionization time-of-flight spectrometer peptide mass fingerprinting being used to determine the differentially expressed proteins between normal and injured intestinal mucosa.
  
  
  RESULTS
  Among several protein spots showing differential expression, one, hemopexin (HPX), was identified as upregulated in indomethacin-induced injured intestinal mucosa using the MASCOT search engine.
  
  
  CONCLUSIONS
  HPX was identified as upregulated protein in the small intestine exposed to indomethacin. HPX may be responsible for the development of the intestinal inflammation induced by NSAIDs.
authors.affiliation: Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan. takatomo@koto.kpu-m.ac.jp
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Takagi
authors.forename: Yuji
authors.initials: Y
authors.lastname: Naito
authors.forename: Hitomi
authors.initials: H
authors.lastname: Okada
authors.forename: Megumi
authors.initials: M
authors.lastname: Takaoka
authors.forename: Tomoko
authors.initials: T
authors.lastname: Oya-Ito
authors.forename: Shinya
authors.initials: S
authors.lastname: Yamada
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Hirai
authors.forename: Katsura
authors.initials: K
authors.lastname: Mizushima
authors.forename: Naohisa
authors.initials: N
authors.lastname: Yoshida
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Kamada
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Katada
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Uchiyama
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ishikawa
authors.forename: Osamu
authors.initials: O
authors.lastname: Handa
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Yagi
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Konishi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kokura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ichikawa
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Yoshikawa
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal; D015153:Blotting, Western; D004195:Disease Models, Animal; D015180:Electrophoresis, Gel, Two-Dimensional; D006466:Hemopexin / Q000378:metabolism*; D007082:Ileum / Q000378:metabolism* / Q000473:pathology; D007150:Immunohistochemistry; D007213:Indomethacin; D007413:Intestinal Mucosa / Q000378:metabolism* / Q000473:pathology; D007583:Jejunum / Q000378:metabolism* / Q000473:pathology; D008297:Male; D010437:Peptic Ulcer / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D010449:Peptide Mapping; D040901:Proteomics / Q000379:methods; D051381:Rats; D017208:Rats, Wistar; D012720:Severity of Illness Index; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors; D015854:Up-Regulation
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D006466:Hemopexin; D007213:Indomethacin