title: Evaluation of Diagnos Malaria Stix test (antigen detection assay) for diagnosis of malaria.
abstract: Malaria is one of the most common parasitic infection in India. The diagnosis largely depends on peripheral blood smear examination. Newer diagnostic methods like various antigen detection assays are now in use for prompt diagnosis and treatment. This study was done to determine the effectiveness of Diagnos Malaria Stix (antigen detection) assay in diagnosis of malaria. This involves detection of PfHRP-2 antigen and P.V. specific pLDH antigen. 162 patients with signs and symptoms of malaria included in the study. Leishman stained blood smear examination was done for all patients. Commercially available Diagnos Malaria Stix assay was used. Diagnos Malaria Stix showed sensitivity, specificity positive and negative predictive values of 100% each while Sensitivity, specificity, positive and negative predictive values of Leishman stained blood smear examination were 45.45%, 100%, 100% and 92% respectively.
authors.affiliation: Dept. of Microbiology, J.N. Medical College, Aligarh, UP.
authors.forename: Haris M
authors.initials: HM
authors.lastname: Khan
authors.forename: Fatima
authors.initials: F
authors.lastname: Shujatullah
authors.forename: M
authors.initials: M
authors.lastname: Shahid
authors.forename: Adil
authors.initials: A
authors.lastname: Raza
authors.forename: Ritu
authors.initials: R
authors.lastname: Malik
mesh_terms: D000953:Antigens, Protozoan / Q000097:blood*; D006801:Humans; D007118:Immunoassay; D008288:Malaria / Q000175:diagnosis*; D010966:Plasmodium vivax / Q000276:immunology*; D011237:Predictive Value of Tests; D015800:Protozoan Proteins / Q000097:blood*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
chemical_list: D000953:Antigens, Protozoan; C514457:HRP-2 antigen, Plasmodium falciparum; D015800:Protozoan Proteins; D011933:Reagent Kits, Diagnostic