title: Preparation of Fe3O4 nanoparticle enclosure hydroxylated multi-walled carbon nanotubes for the determination of aconitines in human serum samples.
abstract: A magnetic carbon nanomaterial for Fe(3)O(4) enclosure hydroxylated multi-walled carbon nanotubes (Fe(3)O(4)-EC-MWCNTs-OH) was prepared by the aggregating effect of Fe(3)O(4) nanoparticle on MWCNTs-OH, and combined with high-performance liquid chromatography (HPLC)/diode array detection (DAD) to determine the aconitines (aconitine, hypaconitine and mesaconitine) in human serum samples. Compared with other extraction modes investigated in experiment, Fe(3)O(4)-EC-MWCNTs-OH sorbents showed a good affinity to target analytes. Some important parameters that could influence extraction efficiency of aconitines, including the extraction mode, amounts of Fe(3)O(4)-EC-MWCNTs-OH, pH of sample solution, extraction time, desorption solvent and desorption time, were optimized. Under optimal conditions, the recoveries of spiked serum samples were between 98.0% and 103.0%; relative standard deviations (RSDs) ranged from 0.9% to 6.2%. The correlation coefficients varied from 0.9996 to 0.9998. The limits of detection ranged from 3.1 ng mL(-1) to 4.1 ng mL(-1) at a signal-to-noise ratio of 3. The experimental results showed that the proposed method was feasible for the analysis of aconitines in serum samples.
authors.affiliation: Key Laboratory of Chemistry of Northwestern Plant Resources, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, PR China.
authors.forename: Hong-Fei
authors.initials: HF
authors.lastname: Zhang
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Shi
mesh_terms: D000157:Aconitine / Q000031:analogs & derivatives* / Q000097:blood; D000327:Adsorption; D002851:Chromatography, High Pressure Liquid; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D044967:Serum; D059629:Signal-To-Noise Ratio; D052616:Solid Phase Extraction / Q000379:methods*; D012997:Solvents
chemical_list: D037742:Nanotubes, Carbon; D012997:Solvents; C058309:hypaconitine; C019470:mesaconitine; D000157:Aconitine; D052203:Ferrosoferric Oxide