title: Discovery of the novel HLA-DRB1*16:16 allele in a Taiwanese unrelated bone marrow stem cell donor by a sequence-based typing method and the probable haplotype associated with DRB1*16:16.
abstract: We report here a de novo HLA-DRB1 allele, DRB1*16:16, discovered in a Taiwanese unrelated volunteer bone marrow stem cell donor by a sequence-based typing (SBT) method. In exon 2, the DNA sequence of DRB1*16:16 is identical to the sequence of DRB1*16:02:01 except the nucleotides at positions 258 (C→T), 260 (C→A) and 261 (T→G). The nucleotide substitution produced an amino acid replacement at residue 58 (A→E). The formation of DRB1*16:16 was probably generated by a DNA sequence recombination event involving DRB1*11:01:01 and DRB1*16:02:01. The probable HLA-A, -B and -DRB1 haplotype in association with DRB1*16:16 may be deduced as A*02-B*38-DRB1*16:16.
authors.affiliation: Laboratory of Immunogenetics and Cord Blood Bank, Buddhist Tzu Chi Stem Cells Centre and Marrow Donor Registry, Buddhist Tzu Chi General Hospital, Buddhist Compassion Relief Tzu Chi Foundation, Hualien, Taiwan. edward@tzuchi.com.tw
authors.forename: K L
authors.initials: KL
authors.lastname: Yang
authors.forename: S K
authors.initials: SK
authors.lastname: Lee
authors.forename: R H
authors.initials: RH
authors.lastname: Kao
authors.forename: C L
authors.initials: CL
authors.lastname: Lin
authors.forename: P Y
authors.initials: PY
authors.lastname: Lin
mesh_terms: D000483:Alleles; D019943:Amino Acid Substitution; D001483:Base Sequence; D001853:Bone Marrow / Q000378:metabolism; D005091:Exons; D059811:HLA-DRB1 Chains / Q000235:genetics*; D006239:Haplotypes; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D008969:Molecular Sequence Data; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA / Q000379:methods; D013624:Taiwan; D014019:Tissue Donors
chemical_list: D059811:HLA-DRB1 Chains