title: Mast cell chymase promotes cell proliferation and expression of certain             cytokines in a dose-dependent manner.
abstract: Wound healing is a complex process, with various intracellular molecules and cytokines involved. Chymase is a chymotrypsin-like serine protease originally found to be a mast cell protease. In this study, to further investigate the role of chymase in the process of wound healing, the effects of chymase on cell proliferation and cytokine activation in human skin fibroblasts were determined. To determine cell proliferation, MTT assay was employed. The cells were harvested and total proteins were extracted and detected by western blotting. It was found that in vitro cell treatments with chymase led to dose-dependent increases of skin fibroblast proliferation. Moreover, the treatment of cells with chymase for 6 h induced dose-dependent increases in the expression levels of transforming growth factor (TGF)-β1 and interleukin (IL)-1β, although higher doses (120 ng/ml for TGF-β1; 60 and 120 ng/ml for IL-1β) did not facilitate the detected cytokine expression. In addition, treatment for longer periods of time (12 or 24 h) did not increase TGF-β1 or IL-1β expression. The results from our study will strongly facilitate the understanding of the roles of chymase in the process of wound healing.
authors.affiliation: Department of Burns and Plastic Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan, People's Republic of China.
authors.forename: Xianglin
authors.initials: X
authors.lastname: Dong
authors.forename: Junjie
authors.initials: J
authors.lastname: Chen
authors.forename: Yange
authors.initials: Y
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Cen
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects; D002648:Child; D002675:Child, Preschool; D053818:Chymases / Q000494:pharmacology*; D003094:Collagen / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism*; D005347:Fibroblasts / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D053583:Interleukin-1beta / Q000378:metabolism; D008407:Mast Cells / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D012867:Skin / Q000166:cytology / Q000187:drug effects; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
chemical_list: D016207:Cytokines; D053583:Interleukin-1beta; D053773:Transforming Growth Factor beta1; D003094:Collagen; D053818:Chymases