title: Models for the mechanism for activating copper-zinc superoxide dismutase in the absence of the CCS Cu chaperone in Arabidopsis.
abstract: Copper-zinc superoxide dismutase (CuZnSOD; CSD) is an important antioxidant enzyme for oxidative stress protection. To date, two activation pathways have been identified in many species. One requiring the CCS, Cu chaperone for SOD, to insert Cu and activate CSD (referred to as CCS-dependent pathway), and the other works independently of CCS (referred to as CCS-independent pathway). In our previous study, we suggest an unidentified factor will work with glutathione (GSH) for CSD activation in the absence of the CCS. Here, two models of the CCS-independent mechanism are proposed. The role of the unidentified factor may work as a scaffold protein, which provides a platform for the CSD protein and Cu-GSH to interact, or as a Cu carrier, which itself can bind Cu and interact with CSD proteins. We also suggest that the CSD protein conformation at C-terminal is important in providing a docking site for unidentified factor to access.
authors.affiliation: Institute of Plant Biology and Department of Life Science, National Taiwan University, Taipei, Taiwan.
authors.forename: Chien-Hsun
authors.initials: CH
authors.lastname: Huang
authors.forename: Wen-Yu
authors.initials: WY
authors.lastname: Kuo
authors.forename: Tsung-Luo
authors.initials: TL
authors.lastname: Jinn
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D003300:Copper / Q000378:metabolism; D018832:Molecular Chaperones / Q000378:metabolism*; D011485:Protein Binding; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; C500748:CCS protein, Arabidopsis; D018832:Molecular Chaperones; D003300:Copper; D013482:Superoxide Dismutase