title: Proteomic analysis of Terminalia chebula extract-dependent changes in human lymphoblastic T cell protein expression.
abstract: Terminalia chebula is a native plant from southern Asia to southwestern China that is used in traditional medicine for the treatment of malignant tumors and diabetes. This plant also has antibacterial and immunomodulatory properties. The present study assessed T. chebula extract-dependent protein expression changes in Jurkat cells. Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry and Ingenuity Pathways Analysis (IPA) were performed to assess protein expression and networks, respectively. A comparative proteomic profile was determined in T. chebula extract (50 μg/mL)-treated and control cells; the expressions of β-tubulin, ring finger and CHY zinc finger domain containing 1, and insulin-like growth factor 1 receptor kinase were significantly down-regulated in T. chebula extract-treated Jurkat cells. Moreover, the molecular basis for the T. chebula extract-dependent protein expression changes in Jurkat cells was determined by IPA. Treatment with the T. chebula extract significantly inhibited nuclear factor-κB activity and affected the proteomic profile of Jurkat cells. The molecular network signatures and functional proteomics obtained in this study may facilitate the evaluation of potential antitumor therapeutic targets and elucidate the molecular mechanism of T. chebula extract-dependent effects in Jurkat cells.
authors.affiliation: Division of Molecular & Life Science, Hanyang University, Ansan, Korea.
authors.forename: Nando Dulal
authors.initials: ND
authors.lastname: Das
authors.forename: Kyoung Hwa
authors.initials: KH
authors.lastname: Jung
authors.forename: Ji Hyun
authors.initials: JH
authors.lastname: Park
authors.forename: Mi Ran
authors.initials: MR
authors.lastname: Choi
authors.forename: Hyung Tae
authors.initials: HT
authors.lastname: Lee
authors.forename: Moo Sung
authors.initials: MS
authors.lastname: Kim
authors.forename: Sang Rin
authors.initials: SR
authors.lastname: Lee
authors.forename: Young Gyu
authors.initials: YG
authors.lastname: Chai
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D019169:Jurkat Cells; D015458:Leukemia, T-Cell / Q000188:drug therapy / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D017526:Receptor, IGF Type 1 / Q000378:metabolism; D013601:T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D029766:Terminalia; D014404:Tubulin / Q000378:metabolism; D016335:Zinc Fingers / Q000502:physiology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D016328:NF-kappa B; D010936:Plant Extracts; D020543:Proteome; D014404:Tubulin; D010770:Phosphotransferases; D017526:Receptor, IGF Type 1