title: Analysis of insect cuticular compounds by non-lethal solid phase micro extraction with styrene-divinylbenzene copolymers.
abstract: Insect cuticular hydrocarbons including relatively non-volatile chemicals play important roles in cuticle protection and chemical communication. The conventional procedures for extracting cuticular compounds from insects require toxic solvents, or non-destructive techniques that do not allow storage of subsequent samples, such as the use of SPME fibers. In this study, we describe and tested a non-lethal process for extracting cuticular hydrocarbons with styrene-divinylbenzene copolymers, and illustrate the method with two species of bees and one species of beetle. The results demonstrate that these compounds can be efficiently trapped by Chromosorb® (SUPELCO) and that this method can be used as an alternative to existing methods.
authors.affiliation: Departamento de Biologia da Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Av. Bandeirantes, Ribeirão Preto, São Paulo, 3900, Brazil.
authors.forename: M J
authors.initials: MJ
authors.lastname: Ferreira-Caliman
authors.forename: I C C
authors.initials: IC
authors.lastname: Turatti
authors.forename: N P
authors.initials: NP
authors.lastname: Lopes
authors.forename: R
authors.initials: R
authors.lastname: Zucchi
authors.forename: F S
authors.initials: FS
authors.lastname: Nascimento
mesh_terms: D000818:Animals; D001516:Bees / Q000737:chemistry*; D055772:Green Chemistry Technology / Q000379:methods*; D006838:Hydrocarbons / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*; D011137:Polystyrenes / Q000737:chemistry*; D052617:Solid Phase Microextraction / Q000379:methods*; D013712:Tenebrio / Q000737:chemistry*
chemical_list: D006838:Hydrocarbons; D011137:Polystyrenes; C432006:divinylbenzene-polystyrene copolymer