title: [Expression of MADD in lung adenocarcinoma tissues and its effects on proliferation and apoptosis of lung adenocarcinoma A549 cells].
abstract: OBJECTIVE
  To investigate the expression of MAPK-activating death domain protein (MADD) in lung adenocarcinoma tissues and its effects on proliferation and apoptosis of lung adenocarcinoma A549 cells.
  
  
  METHODS
  Immunohistochemistry was used to detect the expression of MADD in lung normal and tumor tissues. The expression of IG20 gene in A549 cells was measured by reverse transcription polymerase chain reaction. A549 cells were transfected with pEYFP-MADD plasmids carrying MADD gene or pNL-SIN-GFP-MID lentiviral vectors used for RNA interference.   MADD expression and cell proliferation and apoptosis were determined by Western blot, MTT assay, and flow cytometry.
  
  
  RESULTS
  The expression levels of MADD were higher in lung adenocarcinoma and squamous cell carcinoma tissues than that in lung normal tissues, and lung adenocarcinoma tissues expressed more MADD than lung squamous cell carcinoma tissues. The transcript encoding MADD was expressed in A549 cells. The transfection of pEYFP-MADD plasmids could increase MADD expression  and cell    proliferation   of  A549 cells,   while the A549 cells transfected with pNL-SIN-GFP-MID lentiviral vectors showed significantly  decreases in the MADD level and proliferation. It is shown that MADD overexpression could inhibit A549 cell apoptosis, and knock down of MADD could promote apoptosis of them.
  
  
  CONCLUSIONS
  The expression of MADD increases obviously in lung adenocarcinoma, and MADD can promote survival of lung adenocarcinoma cells by inhibiting apoptosis.
authors.affiliation: Institute of Biochemistry and Molecular Biology, School of Medicine, Qilu Hospital, Shandong University, Jinan 250012, China.
authors.forename: Yu-Ping
authors.initials: YP
authors.lastname: Wei
authors.forename: Jin-Xiang
authors.initials: JX
authors.lastname: Wu
authors.forename: Yuan-Fang
authors.initials: YF
authors.lastname: Guo
authors.forename: Gao-Ying
authors.initials: GY
authors.lastname: Sun
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhang
authors.forename: Wen-Xiang
authors.initials: WX
authors.lastname: Bi
authors.forename: Liang
authors.initials: L
authors.lastname: Dong
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D017209:Apoptosis / Q000235:genetics*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D053418:Death Domain Receptor Signaling Adaptor Proteins / Q000235:genetics* / Q000378:metabolism; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D020662:Guanine Nucleotide Exchange Factors / Q000235:genetics* / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D007150:Immunohistochemistry; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
chemical_list: D053418:Death Domain Receptor Signaling Adaptor Proteins; D020662:Guanine Nucleotide Exchange Factors; D008164:Luminescent Proteins; C105852:MADD protein, human