title: Amount of CD4+CD25+ regulatory T cells in autoimmune pancreatitis and pilonidal sinus.
abstract: OBJECTIVE
  Infiltration of many IgG4-positive plasma cells (G4-Ps) is seen in IgG4-related diseases and in several "non-IgG4-related diseases," such as pilonidal sinus (PS) as well. The involvement of CD4+CD25+ regulatory T cells (CD4CD25 Tregs) in IgG4-related diseases has been reported. To see whether CD4+CD25+ Tregs are involved in autoimmune pancreatitis (AIP)/non-IgG4-related diseases with many G4-Ps, we investigated the amount of G4-Ps and CD4+CD25+ Tregs histologically in AIP/PS.
  
  
  METHODS
  Four AIP and 10 PS were immunostained with IgG4/Foxp3, a specific marker for CD4+CD25+ Tregs. Double immunohistochemistry and dual fluorescent immunohistochemistry were conducted to see the amount of CD4+CD25+ Tregs.
  
  
  RESULTS
  All AIP and 30% of PS showed abundant G4-Ps. G4-Ps infiltrated diffusely for all AIPs and in a patchy pattern for PS at the abscess/granulation foci. Foxp3 immunostaining/double immunohistochemistry showed moderate to abundant CD4+CD25+ Tregs in AIP and abscess of PS, but few to moderate in granulation of PS. Dual fluorescent immunohistochemistry also showed many CD4+CD25+ Tregs in AIP.
  
  
  CONCLUSIONS
  Many CD4+CD25+ Tregs were seen in AIP lesions, abscess of PS, but not in granulation of PS, suggesting that the amount of CD4+CD25+ Tregs sometimes do not synchronize with that of G4-Ps and might relate to the inflammatory activity of both AIP and PS.
authors.affiliation: Department of Human Pathology, Juntendo University School of Medicinel, Tokyo, Japan. yfuku@juntendo.ac.jp
authors.forename: Yuki
authors.initials: Y
authors.lastname: Fukumura
authors.forename: Masaru
authors.initials: M
authors.lastname: Takase
authors.forename: Keiko
authors.initials: K
authors.lastname: Mitani
authors.forename: Koichi
authors.initials: K
authors.lastname: Suda
authors.forename: Abdukadir
authors.initials: A
authors.lastname: Imamhasan
authors.forename: Bunsei
authors.initials: B
authors.lastname: Nobukawa
authors.forename: Akiko
authors.initials: A
authors.lastname: Ueda
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Abe
authors.forename: Takashi
authors.initials: T
authors.lastname: Yao
mesh_terms: D000328:Adult; D000368:Aged; D001327:Autoimmune Diseases / Q000276:immunology*; D015415:Biomarkers / Q000032:analysis; D005260:Female; D051858:Forkhead Transcription Factors / Q000032:analysis; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007150:Immunohistochemistry; D053645:Interleukin-2 Receptor alpha Subunit / Q000032:analysis*; D007564:Japan; D008297:Male; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D010195:Pancreatitis / Q000276:immunology*; D010864:Pilonidal Sinus / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D055815:Young Adult
chemical_list: D015415:Biomarkers; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; C508017:IL2RA protein, human; D007074:Immunoglobulin G; D053645:Interleukin-2 Receptor alpha Subunit