title: Pantothenic acid (vitamin B5) in fortified foods: comparison of a novel ultra-performance liquid chromatography-tandem mass spectrometry method and a microbiological assay (AOAC Official Method 992.07).
abstract: A novel method was developed and single-laboratory validated for the determination of free pantothenic acid (vitamin B5) in a wide range of infant and adult fortified food products. The method combines simple sample preparation and chromatographic analysis using ultra-performance LC coupled to tandem MS with positive electrospray ionization. Pantothenic acid was quantified using [13C6, 15N2]-pantothenic acid as an internal standard. Calibration curves were linear between 0.08 and 1.2 microg/mL (r2 = 0.9998), and average recovery varied between 95 and 106%. The method exhibited overall RSD(r) of 1.1% and RSD intermediate reproducibility from 2.5 to 6.0% in infant formulas and cereals. Comparison of results between total and free pantothenic acid showed that the analysis of free pantothenic acid gave a good estimation of total pantothenic acid in the range of products analyzed. The method provides reliable free pantothenic acid results in a wide range of fortified foods (infant and adult nutritionals, cereal products and beverages), and shows good correlation with the microbiological method AOAC Official Method 992.07. It is a more selective, faster, and robust alternative to microbiological determination.
authors.affiliation: Nestlé Research Center, Vers-chez-les-blanc, CH-1000 Lausanne 26, Switzerland.
authors.forename: Pierre
authors.initials: P
authors.lastname: Andrieux
authors.forename: Patric
authors.initials: P
authors.lastname: Fontannaz
authors.forename: Tamara
authors.initials: T
authors.lastname: Kilinc
authors.forename: Esther Campos
authors.initials: EC
authors.lastname: Giménez
mesh_terms: D000465:Algorithms; D001681:Biological Assay / Q000379:methods; D002021:Buffers; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D005527:Food, Fortified / Q000032:analysis*; D007202:Indicators and Reagents; D007225:Infant Food / Q000032:analysis; D048191:Lactiplantibacillus plantarum / Q000737:chemistry / Q000378:metabolism; D010205:Pantothenic Acid / Q000032:analysis*; D012015:Reference Standards; D012044:Regression Analysis; D015203:Reproducibility of Results; D012996:Solutions / Q000032:analysis; D012997:Solvents; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D002021:Buffers; D007202:Indicators and Reagents; D012996:Solutions; D012997:Solvents; D010205:Pantothenic Acid