title: (1)H, (13)C and (15)N resonance assignments of the C-terminal DNA-binding domain of RstA protein from Klebsiella pneumoniae.
abstract: Bacterial cells often use two-component signal transduction systems to regulate genes in response to environmental stimuli. The RstA/RstB system is a two-component regulatory system consisting of the membrane sensor, RstB, and its cognate response regulator RstA. The RstA of Klebsiella pneumoniae consists of a N-terminal receiver domain (NRD, residues 1-119) and a C-terminal DNA-binding domain (DBD, residues 130-236). Phosphorylation of the response regulator induces a conformational change in the regulatory domain of RstA, which results in activation of the effector domain to regulate the downstream genes, including the ferrous iron transport system (Feo), at low-pH condition. Here we report the (1)H, (13)C and (15)N resonance assignments and secondary structure identification of the DBD of RstA from K. pneumoniae as a first step for unraveling the structural and functional relationship of the RstA/RstB two component system.
authors.affiliation: Institute of Biomedical Sciences, Academia Sinica, Taipei, 115, Taiwan, ROC.
authors.forename: Sheng-Chia
authors.initials: SC
authors.lastname: Chen
authors.forename: Chi-Fon
authors.initials: CF
authors.lastname: Chang
authors.forename: Pei-Ju
authors.initials: PJ
authors.lastname: Fan
authors.forename: Ya-Hsin
authors.initials: YH
authors.lastname: Cheng
authors.forename: Tsunai
authors.initials: T
authors.lastname: Yu
authors.forename: Tai-Huang
authors.initials: TH
authors.lastname: Huang
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D007711:Klebsiella pneumoniae; D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary
chemical_list: D001426:Bacterial Proteins; D004247:DNA