title: Biochemical characterization of murine Ia antigens with xenoantisera. I. Identification of a second la molecule (I-E?) in H-2s haplotype.
abstract: Rabbit anti-Ia sera was produced by immunization with detergent-solubilized extracts from splenic, lymph-node and thymus cells. The antisera contained activity against H-2 as well as Ia molecules. By a sequential immunoprecipitation assay it was shown that the rabbit anti-mouse H-2s serum precipitated a second Ia molecule in the H-2s haplotype. Previous studies with alloantisera have shown only one Ia molecule associated with this haplotype. Sequential precipitations with alloantiserum against the whole I region were used to show that this second Ia molecule is coded by genes within the I region. Since only I-A- and I-E-region coded molecules are immunoprecipitable in most haplotypes, we presume that the rabbit antiserum could be identifying the I-E-subregion coded molecule in the H-2s haplotype. The rabbit antiserum reacts with an isotypic specificity on the molecule. The studies suggest that the I-E subregion does exist in the H-2s haplotype even though alloantiserum cannot be produced to identify allotypic variants associated with this subregion.
authors.affiliation: Department of Immunology, Mayo Clinic and Foundation, Rochester, Minnesota 55901, USA.
authors.forename: W P
authors.initials: WP
authors.lastname: Lafuse
authors.forename: B C
authors.initials: BC
authors.lastname: Neely
authors.forename: C S
authors.initials: CS
authors.lastname: David
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D002874:Chromosome Mapping; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D006183:H-2 Antigens / Q000235:genetics* / Q000276:immunology*; D006239:Haplotypes; D000949:Histocompatibility Antigens Class II / Q000235:genetics* / Q000276:immunology*; D007106:Immune Sera / Q000276:immunology; D047468:Immunoprecipitation / Q000379:methods; D051379:Mice; D008809:Mice, Inbred C3H; D011817:Rabbits; D013211:Staphylococcus aureus
chemical_list: D000939:Epitopes; D006183:H-2 Antigens; D000949:Histocompatibility Antigens Class II; C024138:I-E-antigen; D007106:Immune Sera