title: Elaboration and characterization of nanoliposome made of soya; rapeseed and salmon lecithins: application to cell culture.
abstract: Health benefits of unsaturated fatty acids have been demonstrated over the last decades. Nanotechnology provided new process to produce particles such as liposomes and nanoliposomes made of pure phospholipids. These techniques are already used in pharmaceutics to augment the bioavailability and the bioefficiency of drugs. The aim of this paper is to characterize and evaluate the potential of nanoliposomes made of three lecithins (soya, rapeseed and salmon) on cell culture in order to use them in the future as drug delivery systems for tissue engineering. We began to measure, with zetasizer, the radius size of liposomes particles which are 125.5, 136.7 and 130.3 nm respectively for rapeseed, soya and salmon lecithin. Simultaneously, solutions observed by TEM demonstrated the particles were made much of liposomes than droplet (emulsion). Finally, we found that the solutions of lecithins were enough stable over 5 days at 37 °C to be used in culture medium. We investigated the effect of soya, rapeseed and salmon lecithin liposome from 2mg/mL to 5.2 μg/mL on metabolic activity and cell proliferation on rat bone marrow stem cells (rBMSC) during 14 days. The results showed that the three lecithins (soya, rapeseed and salmon) improve cell proliferation at different concentration.
authors.affiliation: Laboratoire d'Ingénierie des Biomolécules, Nancy-Université, 2 avenue de Forêt de Haye, 54505 Vandoeuvre-Lès-Nancy, France. elmira.arab-tehrany@ensaia.inpl-nancy.fr
authors.forename: Elmira
authors.initials: E
authors.lastname: Arab Tehrany
authors.forename: Cyril J F
authors.initials: CJ
authors.lastname: Kahn
authors.forename: Christophe
authors.initials: C
authors.lastname: Baravian
authors.forename: Behnoush
authors.initials: B
authors.lastname: Maherani
authors.forename: Nabila
authors.initials: N
authors.lastname: Belhaj
authors.forename: Xiong
authors.initials: X
authors.lastname: Wang
authors.forename: Michel
authors.initials: M
authors.lastname: Linder
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects*; D029741:Brassica rapa / Q000737:chemistry*; D018929:Cell Culture Techniques; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D016503:Drug Delivery Systems; D054709:Lecithins / Q000737:chemistry / Q000494:pharmacology*; D008081:Liposomes / Q000737:chemistry / Q000494:pharmacology*; D008297:Male; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D051381:Rats; D017208:Rats, Wistar; D012474:Salmon; D013024:Soybean Oil / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D013499:Surface Properties
chemical_list: D054709:Lecithins; D008081:Liposomes; D013024:Soybean Oil