title: Characterization of coagulase-positive staphylococci isolated from tank and silo ewe milk.
abstract: The prevalence of coagulase-positive staphylococci (CPS) was studied among 390 samples of ewe milk. Fifty-seven (14.85%) samples of tank milk and all samples (6) of silo milk gave a positive result on Baird-Parker agar base supplemented with rabbit plasma fibrinogen, whereas amplification of the coagulase (coa) gene was successful in 6 (1.56%) samples of tank milk and in all silo samples. Phenotypic and genetic analysis of 153 isolates identified 151 (98.69%) as Staphylococcus aureus. Amplification of the coa gene was positive for 149 isolates (97.39%). The staphylococcal enterotoxin (SE) C gene was detected in 116 strains (75.82%), whereas more than one SE gene was carried in 5 strains (3.26%). None of the isolates harbored the genes for SEE or for methicillin resistance. A high prevalence of CPS carrying enterotoxin genes should be of concern because ewe milk is mainly processed into raw milk cheeses. The detection of the coa gene from milk samples could help to assess the microbiological safety of raw milk intended for direct use in the dairy industry.
authors.affiliation: Department of Food Hygiene and Food Technology, Veterinary Faculty, University of León, E-24071 Spain.
authors.forename: B
authors.initials: B
authors.lastname: Linage
authors.forename: J M
authors.initials: JM
authors.lastname: Rodríguez-Calleja
authors.forename: A
authors.initials: A
authors.lastname: Otero
authors.forename: M L
authors.initials: ML
authors.lastname: García-López
authors.forename: J A
authors.initials: JA
authors.lastname: Santos
mesh_terms: D000818:Animals; D003030:Coagulase / Q000032:analysis* / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis; D004768:Enterotoxins / Q000032:analysis; D005260:Female; D008892:Milk / Q000382:microbiology*; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012756:Sheep / Q000382:microbiology*; D013030:Spain; D013211:Staphylococcus aureus / Q000145:classification / Q000201:enzymology / Q000302:isolation & purification*
chemical_list: D003030:Coagulase; D004269:DNA, Bacterial; D004768:Enterotoxins; C034959:enterotoxin C, staphylococcal