title: The spatial relationship of the viral and H-2 antigens recognized by anti-viral CTLs.
abstract: With the use of monospecific rabbit anti-G protein and mouse monoclonal anti-H-2Kk, we have analyzed the spatial relationship of the serologically defined H-2Kk antigens and the major surface glycoprotein (G protein) of vesicular stomatitis virus (VSV) to those antigens recognized by B10.A (k, d) anti-VSV cytotoxic T lymphocytes (CTLs). The ability of monoclonal anti-H-2Kk or rabbit anti-G protein to inhibit specifically the cytolytic activity of B10.A anti-VSV CTLs indicates that the G protein and the H-2Kk molecules are in close proximity to the viral and H-2Kk antigens recognized by the anti-VSV (CTLs. By the method of sequential immunoprecipitation, we also demonstrated that only 10-30 percent of the serologically defined G and H-2Kk molecules are in the G-H-2Kk complexes.
authors.affiliation: Department of Microbiology and Immunology, Bowman Gray School of Medicine, Winston-Salem, North Carolina 27103, USA.
authors.forename: A H
authors.initials: AH
authors.lastname: Hale
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology*; D045744:Cell Line, Tumor; D006023:Glycoproteins / Q000276:immunology; D006183:H-2 Antigens / Q000276:immunology*; D007518:Isoantibodies / Q000097:blood / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014721:Vesicular stomatitis Indiana virus / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D006183:H-2 Antigens; C024755:H-2K(K) antigen; D007518:Isoantibodies; C030036:anti-H-2 antibodies