title: Orthotopic model of SHG-44 in the enhanced green fluorescent protein nude mouse.
abstract: Naturally fluorescent proteins have been widely used in biological research. In this study, we found that the simple and effective way to obtain enhanced green fluorescent protein (EGFP) nude mice is to cross transgenic EGFP C57BL/6J mice with nude (nu/nu) mice. EGFP expression is identified by tail genotyping. Establishment of the orthotopic EGFP nude mouse model used surgical orthotopic implantation. The morphology and human glioma cell markers, such as glial fibrillary acidic protein (GFAP) and S-100, remain unchanged in this mouse model. The tumor blood vessels obtained from the orthotopic model show brilliant EGFP fluorescence as observed by fluorescence microscopy. These findings suggested that this is an ideal mouse model with which to study interaction among host, tumor, and tumor microenvironment; the findings also suggested that the host (EGFP nude mouse) was involved in tumor angiogenesis.
authors.affiliation: Department of Neurosurgery and Brain & Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou, China.
authors.forename: You-Xin
authors.initials: YX
authors.lastname: Zhou
authors.forename: Xiong-Hui
authors.initials: XH
authors.lastname: Chen
authors.forename: Xue-Shun
authors.initials: XS
authors.lastname: Xie
authors.forename: Yu-Lun
authors.initials: YL
authors.lastname: Huang
authors.forename: Zhi-Mou
authors.initials: ZM
authors.lastname: Xue
authors.forename: Gui-Lin
authors.initials: GL
authors.lastname: Chen
authors.forename: Zhong
authors.initials: Z
authors.lastname: Wang
authors.forename: Jin-Ming
authors.initials: JM
authors.lastname: Chen
authors.forename: Zuo-Peng
authors.initials: ZP
authors.lastname: Su
authors.forename: Zi-Wei
authors.initials: ZW
authors.lastname: Du
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000473:pathology; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D005260:Female; D005910:Glioma / Q000473:pathology; D049452:Green Fluorescent Proteins / Q000096:biosynthesis*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D008822:Mice, Transgenic; D009374:Neoplasms, Experimental; D009389:Neovascularization, Pathologic / Q000473:pathology; D016133:Polymerase Chain Reaction
chemical_list: C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins