title: Analysis of the bactericidal response to an experimental Neisseria meningitidis vesicle vaccine.
abstract: Rabbit immunogenicity studies on an experimental trivalent native outer membrane vesicle vaccine derived from three serogroup B strains were conducted to evaluate the effectiveness of this vaccine at inducing an antibody response with serum bactericidal activity against meningococcal strains of other serogroups in addition to serogroup B strains. The results showed that the vaccine was capable of inducing an effective broad-based bactericidal antibody response in rabbits against a small sample of Neisseria meningitidis strains of serogroups C, W135, and X and, to a lesser extent, serogroups A and Y. Analysis of antibody specificity using a bactericidal depletion assay revealed that antibodies to lipooligosaccharide (LOS), PorA, and NadA induced in rabbits by the experimental trivalent outer membrane vesicle vaccine were responsible for most of the bactericidal activity against strains of the other N. meningitidis serogroups. In the case of serogroup A N. meningitidis strains, the outer membrane antigen NadA was primarily responsible for protection. The outer membrane antigens fHbp and OpcA were also effective in removing some bactericidal activity from the sera.
authors.affiliation: Division of Bacterial and Rickettsial Diseases, Walter Reed Army Institute of Research (WRAIR), Silver Spring, Maryland, USA.
authors.forename: Elizabeth E
authors.initials: EE
authors.lastname: Moran
authors.forename: Robert
authors.initials: R
authors.lastname: Burden
authors.forename: Joseph E
authors.initials: JE
authors.lastname: Labrie
authors.forename: Zhiyun
authors.initials: Z
authors.lastname: Wen
authors.forename: Xin-Min
authors.initials: XM
authors.lastname: Wang
authors.forename: Wendell D
authors.initials: WD
authors.lastname: Zollinger
authors.forename: Lan
authors.initials: L
authors.lastname: Zhang
authors.forename: Valerian B
authors.initials: VB
authors.lastname: Pinto
mesh_terms: D018829:Adhesins, Bacterial / Q000276:immunology; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000276:immunology; D001770:Blood Bactericidal Activity; D005260:Female; D008070:Lipopolysaccharides / Q000276:immunology; D022401:Meningococcal Vaccines / Q000008:administration & dosage / Q000276:immunology*; D009345:Neisseria meningitidis / Q000276:immunology*; D018272:Porins / Q000276:immunology; D011817:Rabbits; D022142:Secretory Vesicles / Q000276:immunology
chemical_list: D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D008070:Lipopolysaccharides; D022401:Meningococcal Vaccines; C501982:NadA protein, Neisseria meningitidis; D018272:Porins; C057699:porin protein, Neisseria