title: Endo-xylanase GH11 activation by the fungal metabolite eugenitin.
abstract: Eugenitin, a chromone derivative and a metabolite of the endophyte Mycoleptodiscus indicus, at 5 mM activated a recombinant GH11 endo-xylanase by 40 %. The in silico prediction of ligand-binding sites on the three-dimensional structure of the endo-xylanase revealed that eugenitin interacts mainly by a hydrogen bond with a serine residue and a stacking interaction of the heterocyclic aromatic ring system with a tryptophan residue. Eugenitin improved the GH11 endo-xylanase activity on different substrates, modified the optimal pH and temperature activities and slightly affected the kinetic parameters of the enzyme.
authors.affiliation: Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, 14040-903, Brazil. andrioliw@yahoo.com.br
authors.forename: Willian J
authors.initials: WJ
authors.lastname: Andrioli
authors.forename: André R L
authors.initials: AR
authors.lastname: Damásio
authors.forename: Tony M
authors.initials: TM
authors.lastname: Silva
authors.forename: Vinícius B
authors.initials: VB
authors.lastname: da Silva
authors.forename: Alexandre
authors.initials: A
authors.lastname: Maller
authors.forename: N P D
authors.initials: NP
authors.lastname: Nanayakkara
authors.forename: Carlos H T P
authors.initials: CH
authors.lastname: Silva
authors.forename: Maria L T M
authors.initials: ML
authors.lastname: Polizeli
authors.forename: Jairo K
authors.initials: JK
authors.lastname: Bastos
mesh_terms: D001203:Ascomycota / Q000737:chemistry*; D001230:Aspergillus / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D002867:Chromones / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry / Q000378:metabolism*; D060026:Endophytes / Q000737:chemistry; D004789:Enzyme Activation; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D008958:Models, Molecular; D012984:Software; D013696:Temperature
chemical_list: D002867:Chromones; D043364:Endo-1,4-beta Xylanases; D004121:Dimethyl Sulfoxide