title: Three-dimensional atlas of iron, copper, and zinc in the mouse cerebrum and brainstem.
abstract: Atlases depicting molecular and functional features of the brain are becoming an integral part of modern neuroscience. In this study we used laser ablation-inductively coupled plasma-mass spectrometry (LA-ICPMS) to quantitatively measure iron (Fe), copper (Cu), and zinc (Zn) levels in a serially sectioned C57BL/6 mouse brain (cerebrum and brainstem). Forty-six sections were analyzed in a single experiment of approximately 158 h in duration. We constructed a 46-plate reference atlas by aligning quantified images of metal distribution with corresponding coronal sections from the Allen Mouse Brain Reference Atlas. The 46 plates were also used to construct three-dimensional models of Fe, Cu, and Zn distribution. This atlas represents the first reconstruction of quantitative trace metal distribution through the brain by LA-ICPMS and will facilitate the study of trace metals in the brain and help to elucidate their role in neurobiology.
authors.affiliation: Elemental Bio-imaging Facility, University of Technology, Sydney, New South Wales, Australia. dominic.hare@uts.edu.au
authors.forename: Dominic J
authors.initials: DJ
authors.lastname: Hare
authors.forename: Jason K
authors.initials: JK
authors.lastname: Lee
authors.forename: Alison D
authors.initials: AD
authors.lastname: Beavis
authors.forename: Amanda
authors.initials: A
authors.lastname: van Gramberg
authors.forename: Jessica
authors.initials: J
authors.lastname: George
authors.forename: Paul A
authors.initials: PA
authors.lastname: Adlard
authors.forename: David I
authors.initials: DI
authors.lastname: Finkelstein
authors.forename: Philip A
authors.initials: PA
authors.lastname: Doble
mesh_terms: D000818:Animals; D001933:Brain Stem / Q000737:chemistry*; D054022:Cerebrum / Q000737:chemistry*; D003300:Copper / Q000032:analysis*; D021621:Imaging, Three-Dimensional / Q000379:methods; D007501:Iron / Q000032:analysis*; D053685:Laser Therapy; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D015032:Zinc / Q000032:analysis*
chemical_list: D003300:Copper; D007501:Iron; D015032:Zinc