title: Oxygen measurements in platelet fluids - a new non-invasive method to detect bacterial contaminations in platelets.
abstract: BACKGROUND
  The residual risk for bacterial contamination in blood components especially in platelets is one to two orders of magnitude higher than for transfusion relevant viral infections. The majority of all bacterial transmitted fatalities occurred at the end of platelet shelf life. Therefore, the maximum shelf life of platelet concentrates (PC) was reduced to 4 days after blood donation in Germany in 2008.
  
  
  METHODS
  A new continuous non-invasive bacterial detection method was developed by O(2) measurements in the platelet fluids and tested with 10 transfusion relevant bacteria species.
  
  
  RESULTS
  The bacterial concentration at the time point of a positive signal of PreSense O(2) ranged between 10(2) and 10(5) CFU mL(-1) . Harmful transfusion-transmitted bacterial infection would have probably been prevented by this novel technology. Only strict anaerobic bacteria strains like Clostridium perfringens were not detected within the study period of 72 h.
  
  
  CONCLUSIONS
  The described non-invasive bacterial detection method represents a new approach to prevent transmission of bacterial infection in platelets. The method is characterised by the advantage that all investigations can be performed until right up to the time of transfusion, and therefore, reduce the risk for sample errors to a minimum.
authors.affiliation: Institute for Transfusion Medicine and Immunohematology, German Red Cross, Johann Wolfgang Goethe University, Frankfurt, Germany.
authors.forename: M M
authors.initials: MM
authors.lastname: Mueller
authors.forename: M K
authors.initials: MK
authors.lastname: Hourfar
authors.forename: E
authors.initials: E
authors.lastname: Huber
authors.forename: W
authors.initials: W
authors.lastname: Sireis
authors.forename: W
authors.initials: W
authors.lastname: Weichert
authors.forename: E
authors.initials: E
authors.lastname: Seifried
authors.forename: T
authors.initials: T
authors.lastname: Tonn
authors.forename: M
authors.initials: M
authors.lastname: Schmidt
mesh_terms: D000332:Aerobiosis; D001409:Bacillus cereus / Q000378:metabolism; D001424:Bacterial Infections / Q000517:prevention & control / Q000635:transmission; D001792:Blood Platelets / Q000737:chemistry / Q000382:microbiology*; D001793:Blood Preservation; D003016:Clostridium perfringens / Q000378:metabolism; D001330:Electronic Data Processing; D004755:Enterobacteriaceae / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D019188:Organ Preservation Solutions / Q000737:chemistry*; D010092:Oximetry / Q000295:instrumentation / Q000379:methods*; D010100:Oxygen / Q000032:analysis*; D010865:Pilot Projects; D017713:Platelet Transfusion / Q000009:adverse effects; D013210:Staphylococcus / Q000378:metabolism; D013997:Time Factors
chemical_list: D019188:Organ Preservation Solutions; D010100:Oxygen