title: Expression of platelet-derived growth factor-B mRNA during vaginal vs. dermal incisional wound healing in the rabbit.
abstract: OBJECTIVE
  The outcome of pelvic reconstructive surgery is largely dependent on the vaginal wound healing process, but this process has not yet been fully elucidated. Platelet-derived growth factor (PDGF) is an important mediator of the wound healing process in cutaneous tissue. We sought to compare PDGF-B mRNA expression in vaginal versus cutaneous incisional wound healing in a rabbit model.
  
  
  METHODS
  Bilateral 6 mm full-thickness circular segments were excised from the vagina and abdominal skin in 36 New Zealand-White female rabbits. Animals were euthanized sequentially before, on the day of and 4, 7, 10, 14, 21, 28 and 35 days after wounding. Their wounds were evaluated for surface area and PDGF-B mRNA expression using real time PCR.
  
  
  RESULTS
  In both tissues PDGF-B mRNA expression increased constantly after wounding, reaching peak levels on day 10, and declined immediately thereafter, reaching minimal values on day 21. In both tissues, the expression of PDGF-B mRNA significantly correlated with the wound closure rate.
  
  
  CONCLUSIONS
  PDGF-B mRNA expression significantly correlates with incisional vaginal and cutaneous wound closure, suggesting that this factor plays an important role in the wound healing process of both tissues.
authors.affiliation: Division of Urogynecology and Reconstructive Pelvic Surgery, Carmel Medical Center, Technion University, Rappaport Faculty of Medicine, Haifa, Israel. yabramov@012.net.il
authors.forename: Yoram
authors.initials: Y
authors.lastname: Abramov
authors.forename: Emmet
authors.initials: E
authors.lastname: Hirsch
authors.forename: Vladimir
authors.initials: V
authors.lastname: Ilievski
authors.forename: Roger P
authors.initials: RP
authors.lastname: Goldberg
authors.forename: Sylvia M
authors.initials: SM
authors.lastname: Botros
authors.forename: Peter K
authors.initials: PK
authors.lastname: Sand
mesh_terms: D000818:Animals; D005260:Female; D020574:Proto-Oncogene Proteins c-sis / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D012867:Skin / Q000378:metabolism*; D014621:Vagina / Q000378:metabolism*; D014945:Wound Healing
chemical_list: D020574:Proto-Oncogene Proteins c-sis; D012333:RNA, Messenger