title: Latent acyclovir-resistant herpes simplex virus type 1 in trigeminal ganglia of immunocompetent individuals.
abstract: Specific mutations within the hypervariable herpes simplex virus (HSV) gene thymidine kinase (TK) gene lead to acyclovir (ACV) resistance. To uncover the existence of latent ACV-resistant (ACV(R)) HSV-1, we determined the genetic and functional variability of the HSV-1 TK gene pool in paired trigeminal ganglia (TG) of 5 immunocompetent individuals. The latent virus pool consisted of a donor-specific HSV-1 quasispecies, including one major ACV-sensitive (ACV(S)) and multiple phylogenetic-related minor ACV(S) and ACV(R) TK variants. Contrary to minor variants, major TK variants were shared between paired TG. The data demonstrate the coexistence of phylogenetic-related ACV(S) and ACV(R) latent HSV-1 in human TG.
authors.affiliation: Department of Virology, Laboratory of Neuro-Oncology and Clinical and Cancer Proteomics, Erasmus Medical Center, The Netherlands.
authors.forename: Monique
authors.initials: M
authors.lastname: van Velzen
authors.forename: Freek B
authors.initials: FB
authors.lastname: van Loenen
authors.forename: Roland J W
authors.initials: RJ
authors.lastname: Meesters
authors.forename: Miranda
authors.initials: M
authors.lastname: de Graaf
authors.forename: Lies
authors.initials: L
authors.lastname: Remeijer
authors.forename: Theo M
authors.initials: TM
authors.lastname: Luider
authors.forename: Albert D M E
authors.initials: AD
authors.lastname: Osterhaus
authors.forename: Georges M G M
authors.initials: GM
authors.lastname: Verjans
mesh_terms: D000212:Acyclovir / Q000494:pharmacology*; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D001344:Autopsy; D001483:Base Sequence; D019556:COS Cells; D002522:Chlorocebus aethiops; D004279:DNA, Viral / Q000235:genetics; D024882:Drug Resistance, Viral / Q000235:genetics; D005260:Female; D006561:Herpes Simplex / Q000188:drug therapy / Q000821:virology*; D018259:Herpesvirus 1, Human / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D006801:Humans; D007121:Immunocompetence; D008297:Male; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny; D015995:Prevalence; D017422:Sequence Analysis, DNA; D013937:Thymidine Kinase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012668:Trigeminal Ganglion / Q000821:virology*; D017735:Virus Latency
chemical_list: D000998:Antiviral Agents; D004279:DNA, Viral; D013937:Thymidine Kinase; D000212:Acyclovir