title: A genetic risk factor for low serum ferritin levels in Danish blood donors.
abstract: BACKGROUND
  Iron deficiency is a frequent side effect of blood donation. In recent years, several studies have described genetic variants associated with iron concentrations. However, the impact of these variants on iron levels is unknown in blood donors. Knowledge of genetic variants that predispose donors to iron deficiency would allow bleeding frequency and iron supplementation to be tailored to the individual donor.
  
  
  METHODS
  The genotypes of five specific single-nucleotide polymorphisms (SNPs) in three genes that have been previously associated with iron status and/or restless leg syndrome (RLS) were investigated in two groups of female blood donors. The first group had low iron stores (serum ferritin ≤ 12 µg/L, n = 657), and the second group had normal to high iron stores (serum ferritin > 30 µg/L, n = 645). Genotype distribution for each of the SNPs was compared between the two groups.
  
  
  RESULTS
  Homozygosity for the T-allele of BTBD9 rs9296249 was associated with lower serum ferritin. The odds ratio for low serum ferritin was 1.35 (95% confidence interval, 1.02-1.77; p = 0.03) when comparing donors with the TT genotype with donors with the CT genotype.
  
  
  CONCLUSIONS
  A frequent polymorphism in BTBD9 was significantly associated with serum ferritin. This polymorphism has previously been associated with RLS, but not low iron stores in blood donors.
authors.affiliation: Department of Clinical Immunology, Capital Region, Copenhagen University Hospital, Denmark. erik.soerensen@rh.regionh.dk
authors.forename: Erik
authors.initials: E
authors.lastname: Sørensen
authors.forename: Katrine
authors.initials: K
authors.lastname: Grau
authors.forename: Trine
authors.initials: T
authors.lastname: Berg
authors.forename: Anne Catrine
authors.initials: AC
authors.lastname: Simonsen
authors.forename: Karin
authors.initials: K
authors.lastname: Magnussen
authors.forename: Christian
authors.initials: C
authors.lastname: Erikstrup
authors.forename: Morten Bagge
authors.initials: MB
authors.lastname: Hansen
authors.forename: Henrik
authors.initials: H
authors.lastname: Ullum
mesh_terms: D000328:Adult; D018798:Anemia, Iron-Deficiency / Q000097:blood / Q000453:epidemiology / Q000235:genetics; D001782:Blood Donors / Q000706:statistics & numerical data*; D003718:Denmark / Q000453:epidemiology; D005260:Female; D005293:Ferritins / Q000097:blood*; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000453:epidemiology / Q000235:genetics; D005838:Genotype; D006720:Homozygote; D006801:Humans; D008875:Middle Aged; D009419:Nerve Tissue Proteins; D020641:Polymorphism, Single Nucleotide; D011237:Predictive Value of Tests; D015995:Prevalence; D012148:Restless Legs Syndrome / Q000453:epidemiology / Q000235:genetics; D012307:Risk Factors; D014157:Transcription Factors / Q000235:genetics*; D055815:Young Adult
chemical_list: C522207:BTBD9 protein, human; D009419:Nerve Tissue Proteins; D014157:Transcription Factors; D005293:Ferritins