title: Nanotopography influences adhesion, spreading, and self-renewal of human embryonic stem cells.
abstract: Human embryonic stem cells (hESCs) have great potentials for future cell-based therapeutics. However, their mechanosensitivity to biophysical signals from the cellular microenvironment is not well characterized. Here we introduced an effective microfabrication strategy for accurate control and patterning of nanoroughness on glass surfaces. Our results demonstrated that nanotopography could provide a potent regulatory signal over different hESC behaviors, including cell morphology, adhesion, proliferation, clonal expansion, and self-renewal. Our results indicated that topological sensing of hESCs might include feedback regulation involving mechanosensory integrin-mediated cell-matrix adhesion, myosin II, and E-cadherin. Our results also demonstrated that cellular responses to nanotopography were cell-type specific, and as such, we could generate a spatially segregated coculture system for hESCs and NIH/3T3 fibroblasts using patterned nanorough glass surfaces.
authors.affiliation: Integrated Biosystems and Biomechanics Laboratory, University of Michigan, Ann Arbor, Michigan 48109, USA.
authors.forename: Weiqiang
authors.initials: W
authors.lastname: Chen
authors.forename: Luis G
authors.initials: LG
authors.lastname: Villa-Diaz
authors.forename: Yubing
authors.initials: Y
authors.lastname: Sun
authors.forename: Shinuo
authors.initials: S
authors.lastname: Weng
authors.forename: Jin Koo
authors.initials: JK
authors.lastname: Kim
authors.forename: Raymond H W
authors.initials: RH
authors.lastname: Lam
authors.forename: Lin
authors.initials: L
authors.lastname: Han
authors.forename: Rong
authors.initials: R
authors.lastname: Fan
authors.forename: Paul H
authors.initials: PH
authors.lastname: Krebsbach
authors.forename: Jianping
authors.initials: J
authors.lastname: Fu
mesh_terms: D015153:Blotting, Western; D002448:Cell Adhesion; D002465:Cell Movement; D053595:Embryonic Stem Cells / Q000166:cytology*; D005455:Fluorescent Antibody Technique; D006801:Humans