title: Determination of total choline by liquid chromatography-electrospray ionization-tandem mass spectrometry in infant formulas.
abstract: Choline is a water-soluble nutrient important for infants' brain and neural development. In infant formulas, choline is one of the important fortified nutrients. A single-laboratory validation study conducted an LC-electrospray ionization-MS/MS to determine total choline in infant formulas. Sample preparation was adopted from AOAC Official Method 999.14, and instrumental running conditions were optimized. The LOQ was 0.2 microg/100 g, which is significant for measuring total choline in infant formulas. Average recoveries for milk-, rice-, soybean-, and hydrolyzed protein-based samples ranged from 86.45 +/- 6.04% to 108.98 +/- 3.68%, with RSD less than 7%. The repeatability RSD (RSD(r)) range was 0.24-3.59% in within-day evaluation and 1.16-3.24% in day-to-day evaluation. Matrix effect was also investigated, and can be effectively eliminated by using an internal standard. Therefore, this method has high credibility, and could be used as a routine method of quality control, or for clinical studies and other research areas.
authors.affiliation: Zhejiang Beingmate Technology Industry and Trade Co., Ltd, Beingmate Research Institute, 3875 Nanhuan Rd, Bingjiang, Hangzhou, China 310057. fss820312@gmail.com
authors.forename: Shishan
authors.initials: S
authors.lastname: Fu
authors.forename: Baohua
authors.initials: B
authors.lastname: Tao
authors.forename: Shiyun
authors.initials: S
authors.lastname: Lai
authors.forename: Jingshun
authors.initials: J
authors.lastname: Zhang
authors.forename: Ren
authors.initials: R
authors.lastname: Yiping
mesh_terms: D000818:Animals; D002138:Calibration; D002417:Cattle; D002794:Choline / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D007202:Indicators and Reagents; D007223:Infant; D007225:Infant Food / Q000032:analysis*; D007231:Infant, Newborn; D057230:Limit of Detection; D008892:Milk / Q000737:chemistry; D012275:Oryza / Q000737:chemistry; D010739:Phospholipase D / Q000737:chemistry; D011492:Protein Hydrolysates / Q000032:analysis; D012015:Reference Standards; D015203:Reproducibility of Results; D013025:Glycine max / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D007202:Indicators and Reagents; D011492:Protein Hydrolysates; D010739:Phospholipase D; D002794:Choline