title: Imaging protein interactions by FRET microscopy: FLIM measurements.
abstract: This image acquisition protocol is a basic plan for taking a fluorescence lifetime imaging (FLIM) series. FLIM makes live-cell FRET measurements based only on donor fluorescence more feasible, because lifetimes are independent of probe concentration and light path length. The former is not easy to determine in cells, and the latter means that cell shape is not a factor.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Verveer
authors.forename: Oliver
authors.initials: O
authors.lastname: Rocks
authors.forename: Ailsa G
authors.initials: AG
authors.lastname: Harpur
authors.forename: Philippe I H
authors.initials: PI
authors.lastname: Bastiaens