title: Phylogenetic diversity of the dddP gene for dimethylsulfoniopropionate-dependent dimethyl sulfide synthesis in mangrove soils.
abstract: The dddP gene encodes an enzyme that cleaves dimethylsulfoniopropionate (DMSP) into dimethyl sulfide (DMS) plus acrylate and has been identified in various marine bacteria and some fungi. The diversity of dddP genes was investigated by culture-independent PCR-based analysis of metagenomic DNA extracted from 4 mangrove soils in Southern China. A phylogenetic tree of 144 cloned dddP sequences comprised 7 groups, 3 of which also included dddP genes from previously identified Ddd(+) (DMSP-dependent DMS production) bacteria. However, most (69%) of the DddP sequences from the mangroves were in 4 other subgroups that did not include sequences from known bacteria, demonstrating a high level of diversity of this gene in these environments. Each clade contained clones from all of the sample sites, suggesting that different dddP types are widespread in mangroves of different geographical locations. Furthermore, it was found the dddP genotype distribution was remarkably influenced by the soil properties pH, available sulfur, salt, and total nitrogen.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, People's Republic of China.
authors.forename: Mengjun
authors.initials: M
authors.lastname: Peng
authors.forename: Qingyi
authors.initials: Q
authors.lastname: Xie
authors.forename: Huo
authors.initials: H
authors.lastname: Hu
authors.forename: Kui
authors.initials: K
authors.lastname: Hong
authors.forename: Jonathan D
authors.initials: JD
authors.lastname: Todd
authors.forename: Andrew W B
authors.initials: AW
authors.lastname: Johnston
authors.forename: Youguo
authors.initials: Y
authors.lastname: Li
mesh_terms: D032407:Avicennia / Q000254:growth & development / Q000382:microbiology; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D002681:China; D017753:Ecosystem; D014644:Genetic Variation; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000378:metabolism; D010802:Phylogeny; D058441:Rhizosphere; D054712:Salinity; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology; D013440:Sulfides / Q000032:analysis / Q000378:metabolism*; D013452:Sulfonium Compounds / Q000032:analysis / Q000378:metabolism*; D013455:Sulfur / Q000378:metabolism
chemical_list: D012987:Soil; D013440:Sulfides; D013452:Sulfonium Compounds; D013455:Sulfur; C068078:dimethylpropiothetin; D009584:Nitrogen; C004784:dimethyl sulfide