title: Design, synthesis and in vivo/in vitro screening of novel chlorokojic acid derivatives.
abstract: A series of novel Mannich bases of chlorokojic acid (2-chloromethyl-5-hydroxy-4H-pyran-4-one) were synthesized and their biological activities were investigated. Anticonvulsant activity results according to phase-I tests of Antiepileptic Drug Development (ADD) Program revealed that compound 13 was the most effective one at 4 h against subcutaneous pentylenetetrazole (scPTZ)-induced seizure test. Antimicrobial activities were evaluated in vitro against bacteria and fungi by using broth microdilution method. The antitubercular activities against Mycobacterium tuberculosis and M. avium were discussed with Resazurin microplate assay (REMA). The antimicrobial activity results indicated that compounds 1 and 12 (MIC: 8-16 µg/mL) showed higher activity against Gram negative bacteria while compound 12 had MIC: 4-16 µg/mL against Gram positive bacteria. Compound 1 was the most active one with MIC values of 8-32 µg/mL against fungi. Mannich bases also exhibit significant antitubercular activity in a MIC range of 4 to 32 µg/mL, especially compound 18 against M. avium.
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Hacettepe University, Ankara, Turkey.
authors.forename: Gülşah
authors.initials: G
authors.lastname: Karakaya
authors.forename: Mutlu Dilsiz
authors.initials: MD
authors.lastname: Aytemir
authors.forename: Berrin
authors.initials: B
authors.lastname: Özçelik
authors.forename: Ünsal
authors.initials: Ü
authors.lastname: Calış
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000138:chemical synthesis* / Q000494:pharmacology*; D000927:Anticonvulsants / Q000138:chemical synthesis* / Q000494:pharmacology*; D000995:Antitubercular Agents / Q000494:pharmacology; D060326:Chemistry Techniques, Synthetic; D015195:Drug Design; D004353:Drug Evaluation, Preclinical / Q000379:methods; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D008297:Male; D008352:Mannich Bases / Q000737:chemistry; D051379:Mice; D008826:Microbial Sensitivity Tests; D009162:Mycobacterium avium / Q000187:drug effects; D009169:Mycobacterium tuberculosis / Q000187:drug effects; D010433:Pentylenetetrazole / Q000633:toxicity; D011753:Pyrones / Q000737:chemistry*; D012640:Seizures / Q000139:chemically induced / Q000188:drug therapy
chemical_list: D000890:Anti-Infective Agents; D000927:Anticonvulsants; D000995:Antitubercular Agents; D008352:Mannich Bases; D011753:Pyrones; C116986:2-chloromethyl-5-hydroxy-4-pyranone; D010433:Pentylenetetrazole