title: Action of sisal (Agave sisalana, Perrine) extract in the in vitro development of sheep and goat gastrointestinal nematodes.
abstract: Active compounds from Agave sisalana with antiparasitic action against gastrointestinal nematodes (GINs) could be an alternative to diversify the range of parasite management methods in the livestock sector. The objective of this study was to evaluate the in vitro action of A. sisalana extract on the development of sheep and goat GINs. The extract, obtained from shredded sisal leaves, was utilized at various concentrations in the egg hatch test (EHT), larval development test (LDT), larval feeding inhibition test (LFIT) and adult motility test (AMT). The LC(50) and LC(95) in the EHT were 6.90 and 24.79 mg/mL, in the LDT were 0.041 and 0.067 mg/mL and in the LFIT were 0.053 and 0.24 mg/mL, respectively, showing a dose-dependent relationship. The development and feeding inhibition on L(1) were both 100% at a dose of 0.12 mg/mL. In the AMT there was 100% inhibition at 75 mg/mL after 24h of exposure. The extract of A. sisalana therefore demonstrated significant action on L(1) at 0.12 mg/mL. So, if part of the A. sisalana extract passes through the animal's gastrointestinal system, this material can have a significant effect on the parasites in the feces. This is an interesting approach because it can drastically reduce the pasture contamination as well as the infection of herds.
authors.affiliation: Departamento Patologia e Clínicas, Universidade Federal da Bahia, Salvador, Bahia, Brazil.
authors.forename: Roberta X
authors.initials: RX
authors.lastname: Silveira
authors.forename: Ana Carolina S
authors.initials: AC
authors.lastname: Chagas
authors.forename: Mariana B
authors.initials: MB
authors.lastname: Botura
authors.forename: Maria J M
authors.initials: MJ
authors.lastname: Batatinha
authors.forename: Luciana M
authors.initials: LM
authors.lastname: Katiki
authors.forename: Camila O
authors.initials: CO
authors.lastname: Carvalho
authors.forename: Cláudia M L
authors.initials: CM
authors.lastname: Bevilaqua
authors.forename: Alexsandro
authors.initials: A
authors.lastname: Branco
authors.forename: Elane A A
authors.initials: EA
authors.lastname: Machado
authors.forename: Simone L
authors.initials: SL
authors.lastname: Borges
authors.forename: Maria A O
authors.initials: MA
authors.lastname: Almeida
mesh_terms: D027882:Agave / Q000737:chemistry*; D015766:Albendazole / Q000494:pharmacology / Q000627:therapeutic use; D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology / Q000627:therapeutic use; D004305:Dose-Response Relationship, Drug; D005767:Gastrointestinal Diseases / Q000188:drug therapy / Q000469:parasitology / Q000662:veterinary*; D015511:Goat Diseases / Q000188:drug therapy* / Q000469:parasitology; D006041:Goats; D007814:Larva / Q000187:drug effects / Q000502:physiology; D009348:Nematoda / Q000187:drug effects / Q000254:growth & development / Q000502:physiology; D009349:Nematode Infections / Q000188:drug therapy / Q000469:parasitology / Q000662:veterinary*; D010063:Ovum / Q000187:drug effects / Q000502:physiology; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D012756:Sheep; D012757:Sheep Diseases / Q000188:drug therapy* / Q000469:parasitology
chemical_list: D000871:Anthelmintics; D010936:Plant Extracts; D015766:Albendazole