title: 2D gels and their third-dimension potential.
abstract: Two-dimensional (2D) agarose gel electrophoresis is one of the most powerful methods to analyze the mass and shape of replication intermediates. It is often use to map replication origins but it is also useful to characterize termination of replication, replication fork barriers and even replication fork reversal. Here, we present protocols, figures and movies with a thorough description of different modes of replication for linear DNA fragments and the corresponding patterns they generate in 2D gels.
authors.affiliation: Department of Cell Proliferation and Development, Centro de Investigaciones Biológicas (CSIC), Madrid, Spain. schvartzman@cib.csic.es
authors.forename: Jorge B
authors.initials: JB
authors.lastname: Schvartzman
authors.forename: María-Luisa
authors.initials: ML
authors.lastname: Martínez-Robles
authors.forename: Virginia
authors.initials: V
authors.lastname: López
authors.forename: Pablo
authors.initials: P
authors.lastname: Hernández
authors.forename: Dora B
authors.initials: DB
authors.lastname: Krimer
mesh_terms: D001345:Autoradiography; D004261:DNA Replication; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D004270:DNA, Circular / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel / Q000379:methods; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D018625:Microscopy, Atomic Force; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D010957:Plasmids / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D004270:DNA, Circular