title: Cryptosporidium tyzzeri and Cryptosporidium muris originated from wild West-European house mice (Mus musculus domesticus) and East-European house mice (Mus musculus musculus) are non-infectious for pigs.
abstract: Three and 8 week old pigs were inoculated with Cryptosporidium muris HZ206 (Mus musculus musculus isolate), Cryptosporidium tyzerri CR2090 (M. m. musculus isolate) or C. tyzzeri CR4293 (isolate from a hybrid between Mus musculus domesticus and M. m. musculus) at a dose of 1 × 10(7) oocysts per animal. Inoculated pigs showed no detectable infection and no clinical symptoms of cryptosporidiosis during 30 days post infection (DPI), and no macroscopic changes were detected in the digestive tract following necropsy. Developmental stages were not detected in gastrointestinal tract tissue by histology or PCR throughout the duration of the experiment. The infectivity of isolates was verified on SCID mice, in which oocysts shedding started from 4 to 8 DPI. Based on our findings, it can be concluded that pigs are not susceptible to C. muris or C. tyzzeri infection.
authors.affiliation: Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic, vvi, České Budějovice, Czech Republic. kvac@paru.cas.cz
authors.forename: Martin
authors.initials: M
authors.lastname: Kváč
authors.forename: Michaela
authors.initials: M
authors.lastname: Kestřánová
authors.forename: Dana
authors.initials: D
authors.lastname: Květoňová
authors.forename: Michaela
authors.initials: M
authors.lastname: Kotková
authors.forename: Ynés
authors.initials: Y
authors.lastname: Ortega
authors.forename: John
authors.initials: J
authors.lastname: McEvoy
authors.forename: Bohumil
authors.initials: B
authors.lastname: Sak
mesh_terms: D000818:Animals; D003457:Cryptosporidiosis / Q000469:parasitology / Q000662:veterinary*; D003458:Cryptosporidium / Q000145:classification / Q000235:genetics / Q000502:physiology*; D018153:Czech Republic; D016054:DNA, Protozoan / Q000737:chemistry / Q000302:isolation & purification; D005243:Feces / Q000469:parasitology; D005838:Genotype; D058507:Host Specificity; D051379:Mice / Q000469:parasitology*; D016513:Mice, SCID; D012335:RNA, Ribosomal / Q000235:genetics; D012376:Rodent Diseases / Q000469:parasitology*; D016415:Sequence Alignment / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000469:parasitology*
chemical_list: D016054:DNA, Protozoan; D012335:RNA, Ribosomal