title: Digital polymerase chain reaction in an array of femtoliter polydimethylsiloxane microreactors.
abstract: We developed a simple, compact microfluidic device to perform high dynamic-range digital polymerase chain reaction (dPCR) in an array of isolated 36-femtoliter microreactors. The density of the microreactors exceeded 20000/mm(2). This device, made from polydimethylsiloxane (PDMS), allows the samples to be loaded into all microreactors simultaneously. The microreactors are completely sealed through the deformation of a PDMS membrane. The small volume of the microreactors ensures a compact device with high reaction efficiency and low reagent and sample consumption. Future potential applications of this platform include multicolor dPCR and massively parallel dPCR for next generation sequencing library preparation.
authors.forename: Yongfan
authors.initials: Y
authors.lastname: Men
authors.forename: Yusi
authors.initials: Y
authors.lastname: Fu
authors.forename: Zitian
authors.initials: Z
authors.lastname: Chen
authors.forename: Peter A
authors.initials: PA
authors.lastname: Sims
authors.forename: William J
authors.initials: WJ
authors.lastname: Greenleaf
authors.forename: Yanyi
authors.initials: Y
authors.lastname: Huang
mesh_terms: D000818:Animals; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D018398:Homeodomain Proteins / Q000032:analysis; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D016133:Polymerase Chain Reaction / Q000295:instrumentation*; D014157:Transcription Factors / Q000032:analysis; D000099261:Homeobox A1 Protein
chemical_list: D004129:Dimethylpolysiloxanes; D018398:Homeodomain Proteins; D014157:Transcription Factors; D000099261:Homeobox A1 Protein; C013830:baysilon