title: Phosphorylating reagent-free synthesis of 5'-phosphate oligonucleotides by controlled oxidative degradation of their 5'-end.
abstract: The 5'-phosphorylated oligonucleotides (5'-pONs) are currently synthesized using expensive and sensitive modified phosphoramidite reagents. In this work, a simple, cost-effective, efficient, and automatable method is presented, based on the controlled oxidation of the 5'-terminal alcohol followed by a β-elimination reaction. The latter reaction leads to the removal of the terminal 5'-nucleoside and subsequent formation of the 5'-phosphate moiety. Thus, chemical phosphorylation of oligonucleotides (DNA or RNA) is achieved without using modified phosphoramidites.
authors.affiliation: Département de Chimie Moléculaire, UMR CNRS 5250, Université Joseph Fourier, BP 53, 38041 Grenoble Cedex 9, France.
authors.forename: Corinne
authors.initials: C
authors.lastname: Sallamand
authors.forename: Audrey
authors.initials: A
authors.lastname: Miscioscia
authors.forename: Rémy
authors.initials: R
authors.lastname: Lartia
authors.forename: Eric
authors.initials: E
authors.lastname: Defrancq
mesh_terms: D015394:Molecular Structure; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D010084:Oxidation-Reduction; D010766:Phosphorylation
chemical_list: D009841:Oligonucleotides