title: De-novo assembly and analysis of the heterozygous triploid genome of the wine spoilage yeast Dekkera bruxellensis AWRI1499.
abstract: Despite its industrial importance, the yeast species Dekkera (Brettanomyces) bruxellensis has remained poorly understood at the genetic level. In this study we describe whole genome sequencing and analysis for a prevalent wine spoilage strain, AWRI1499. The 12.7 Mb assembly, consisting of 324 contigs in 99 scaffolds (super-contigs) at 26-fold coverage, exhibits a relatively high density of single nucleotide polymorphisms (SNPs). Haplotype sampling for 1.2% of open reading frames suggested that the D. bruxellensis AWRI1499 genome is comprised of a moderately heterozygous diploid genome, in combination with a divergent haploid genome. Gene content analysis revealed enrichment in membrane proteins, particularly transporters, along with oxidoreductase enzymes. Availability of this assembly and annotation provides a resource for further investigation of genomic organization in this species, and functional characterization of genes that may confer important phenotypic traits.
authors.affiliation: The Australian Wine Research Institute, Adelaide, Australia. chris.curtin@awri.com.au
authors.forename: Chris D
authors.initials: CD
authors.lastname: Curtin
authors.forename: Anthony R
authors.initials: AR
authors.lastname: Borneman
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Chambers
authors.forename: Isak S
authors.initials: IS
authors.lastname: Pretorius
mesh_terms: D055167:Dekkera / Q000145:classification / Q000235:genetics*; D016681:Genome, Fungal; D006239:Haplotypes; D016366:Open Reading Frames; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D014920:Wine / Q000382:microbiology*